WorldWideScience

Sample records for flower extract aloe

  1. (Kaner) Flower Extract

    African Journals Online (AJOL)

    Erah

    Purpose: The purpose of the study was to evaluate the activity of 50 % hydroalcohol flower extract of. Nerium oleander Linn. on the central nervous system (CNS) of mice. Methods: The effect of the 50 % hydroalcohol extract of N. oleander flowers at dosage levels of 100 and 200 mg/kg p.o. on the locomotor activity of mice ...

  2. Aloe plant extracts as alternative larvicides for mosquito control ...

    African Journals Online (AJOL)

    The larvicidal activity of extracts from Aloe turkanensis, Aloe ngongensis and Aloe fibrosa against the common malaria vector, Anopheles gambie, was determined. Ground Aloe leaves from the three plants were sequentially extracted with hexane, ethyl acetate, chloroform, acetone and methanol. Only the ethyl acetate ...

  3. Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

    International Nuclear Information System (INIS)

    Lee, Eun Mi; Bai, Hyoung-Woo; Lee, Seung Sik; Hong, Sung Hyun; Cho, Jae-Young; Byung, Yeoup Chung

    2012-01-01

    Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  4. Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

    Science.gov (United States)

    Mi Lee, Eun; Bai, Hyoung-Woo; Sik Lee, Seung; Hyun Hong, Sung; Cho, Jae-Young; Yeoup Chung, Byung

    2012-08-01

    Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  5. Aloe vera extract activity on human corneal cells.

    Science.gov (United States)

    Woźniak, Anna; Paduch, Roman

    2012-02-01

    Ocular diseases are currently an important problem in modern societies. Patients suffer from various ophthalmologic ailments namely, conjunctivitis, dry eye, dacryocystitis or degenerative diseases. Therefore, there is a need to introduce new treatment methods, including medicinal plants usage. Aloe vera [Aloe barbadensis Miller (Liliaceae)] possesses wound-healing properties and shows immunomodulatory, anti-inflammatory or antioxidant activities. NR uptake, MTT, DPPH• reduction, Griess reaction, ELISA and rhodamine-phalloidin staining were used to test toxicity, antiproliferative activity, reactive oxygen species (ROS) reduction, nitric oxide (NO) and cytokine level, and distribution of F-actin in cells, respectively. The present study analyzes the effect of Aloe vera extracts obtained with different solvents on in vitro culture of human 10.014 pRSV-T corneal cells. We found no toxicity of ethanol, ethyl acetate and heptane extracts of Aloe vera on human corneal cells. No ROS reducing activity by heptane extract and trace action by ethanol (only at high concentration 125 µg/ml) extract of Aloe vera was observed. Only ethyl acetate extract expressed distinct free radical scavenging effect. Plant extracts decreased NO production by human corneal cells as compared to untreated controls. The cytokine (IL-1β, IL-6, TNF-α and IL-10) production decreased after the addition of Aloe vera extracts to the culture media. Aloe vera contains multiple pharmacologically active substances which are capable of modulating cellular phenotypes and functions. Aloe vera ethanol and ethyl acetate extracts may be used in eye drops to treat inflammations and other ailments of external parts of the eye such as the cornea.

  6. Aloe

    Science.gov (United States)

    ... the aloe plant. It is used in many skin care products. Aloe poisoning occurs when someone swallows this substance. ... Diarrhea Loss of vision Rash Severe abdominal pain Skin irritation Throat swelling (which may also cause breathing difficulty) Vomiting

  7. Topical Aloe Vera (Aloe barbadensis Miller) Extract Does Not Accelerate the Oral Wound Healing in Rats.

    Science.gov (United States)

    Coelho, Fernanda Hack; Salvadori, Gabriela; Rados, Pantelis Varvaki; Magnusson, Alessandra; Danilevicz, Chris Krebs; Meurer, Luise; Martins, Manoela Domingues

    2015-07-01

    The effect of topical application of Aloe Vera (Aloe barbadensis Miller) extract was assessed on the healing of rat oral wounds in an in vivo model using 72 male Wistar rats divided into three groups (n = 24): control, placebo and Aloe Vera (0.5% extract hydroalcoholic). Traumatic ulcers were caused in the dorsum of the tongue using a 3-mm punch tool. The Aloe Vera and placebo group received two daily applications. The animals were sacrificed after 1, 5, 10 and 14 days. Clinical analysis (ulcer area and percentage of repair) and histopathological analysis (degree of re-epithelialization and inflammation) were performed. The comparison of the differences between scores based on group and experimental period, both in quantitative and semi-quantitative analyses, was performed using the Kruskal-Wallis test. The significance level was 5%. On day 1, all groups showed predominantly acute inflammatory infiltrate. On day 5, there was partial epithelialization and chronic inflammatory infiltrate. On the days 10 and 14 total repair of ulcers was observed. There was no significant difference between groups in the repair of mouth ulcers. It is concluded that treatment using Aloe Vera as an herbal formulation did not accelerate oral wound healing in rats. Copyright © 2015 John Wiley & Sons, Ltd.

  8. Notes on the flowering and pollination of the endemic grassland Aloe reitzii var. reitzii (Asphodelaceae

    Directory of Open Access Journals (Sweden)

    Craig T. Symes

    2017-08-01

    Conclusion: Because birds are important pollinators for many Aloe species, it is assumed that the bird species detected visiting A. reitzii var. reitzii are similarly important pollinators. At least 10 invertebrate species and sengi (Elephantulus sp. were also recorded as visitors to flowers, but they may be less important pollinators than specialist and generalist avian nectarivores. This study provides further insight into the pollination biology of a diverse, and ecologically important, succulent genus in Africa.

  9. Prevention of the onset of hyperglycaemia by extracts of Aloe ...

    African Journals Online (AJOL)

    GREGO

    The ability of a home-made aqueous extract of Aloe barbadensis to prevent the onset of alloxan- induced hyperglycaemia was examined and compared with that of a factory-produced gel. Three groups of animals were administered 200 mg/kg body weight of alloxan intraperitoneally. A fourth group of animals was left ...

  10. Aloe arborescens aqueous gel extract alters the activities of key ...

    African Journals Online (AJOL)

    The present study investigated the antidiabetic activity and the possible mechanisms of action of aqueous extract of Aloe arborescens leaf gel (AALGEt) on normal and alloxan-induced diabetic rats. Diabetes was induced in 12 h fasted rats by intraperitoneal injection of 140 mg/kg body weight of alloxan. Blood glucose ...

  11. Prevention of the onset of hyperglycaemia by extracts of Aloe ...

    African Journals Online (AJOL)

    The ability of a home-made aqueous extract of Aloe barbadensis to prevent the onset of alloxaninduced hyperglycaemia was examined and compared with that of a factory-produced gel. Three groups of animals were administered 200 mg/kg body weight of alloxan intraperitoneally. A fourth group of animals was left ...

  12. Consumption of aqueous extract of raw Aloe Vera leaves ...

    African Journals Online (AJOL)

    Forty-five juvenile tilapia and 30 weanling albino rats exposed to water containing 50, 100 and 150ppm of aqueous extract of Aloe Vera leaves for 96 hours and 28 days, respectively were used for this study. Fifteen tilapia and 10 rats exposed to clean water (0 ppm A. vera) served as controls. Clinical signs, mortality, gross ...

  13. The Effects of Crude Aqueous and Alcohol Extracts of Aloe Vera on ...

    African Journals Online (AJOL)

    The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day ...

  14. The efficacy of crude extract of Aloe secundiflora on Candida Albicans

    African Journals Online (AJOL)

    In- vitro studies on the efficacy of crude extracts of Aloe secundiflora on Candida albicans was conducted. Five mature leaves of Aloe secundiflora were collected and the crude extract was prepared, then autoclaved. The extract was then tested on Candida albicans grown on solid media. The results from these studies ...

  15. Physicochemical and microbiological properties as well as stability of ointments containing aloe extract (Aloe arborescens Mill.) or aloe extract associated to neomycin sulphate.

    Science.gov (United States)

    Kodym, A; Bujak, T

    2002-12-01

    The aim of the study was to work out methods of quality assessment of ointments containing dry extract from fresh leaves of Aloe arborescens Mill. (Lilliaceae) and also of ointments containing both of dry extract and neomycin sulphate. The stability of the ointments, stored at 20 degrees C, was studied and the following criteria were considered: chromatographic analysis (TLC), pH of the ointments, the content of the substances in the dry extract converted to aloenin, the content of aloenin and aloin, anti-microbial activity of neomycin in the ointments, the size of the particles of the dry extract and of neomycin sulphate in the ointment suspension and the sterility of the ointments. After two years of storage at 20 degrees C, the ointments prepared with the anhydrous lipophilic base, did not change their physicochemical characteristics and neomycin in those ointments retained almost 100% of starting anti-microbial activity. Water or propylene glycol significantly decreased the stability of the biologically active substances of the dry extract in the ointments. Besides, in the ointments containing the dry extract and neomycin sulphate, the presence of water or propylene glycol induced degradation of the biologically active substances of the dry extract and a decrease in the anti-microbial activity of neomycin in the ointments. Considering the physicochemical and microbiological stability, the most advisable base for the ointments with aloe and neomycin sulphate was composed of white vaseline, liquid paraffin, solid paraffin, cholesterol.

  16. The effect of aqueous Aloe vera gel extract on serum mineral ...

    African Journals Online (AJOL)

    This study was conducted to determine the effect of Aloe vera gel extract on the serum mineral compositions of Red Sokoto bucks. Blood samples were collected from 30 bucks before the commencement of administration of Aloe vera gel extract for serum minerals and these served as control group. The bucks were now ...

  17. Anticonvulsant activity of Aloe vera leaf extract in acute and chronic models of epilepsy in mice.

    Science.gov (United States)

    Rathor, Naveen; Arora, Tarun; Manocha, Sachin; Patil, Amol N; Mediratta, Pramod K; Sharma, Krishna K

    2014-03-01

    The effect of Aloe vera in epilepsy has not yet been explored. This study was done to explore the effect of aqueous extract of Aloe vera leaf powder on three acute and one chronic model of epilepsy. In acute study, aqueous extract of Aloe vera leaf (extract) powder was administered in doses 100, 200 and 400 mg/kg p.o. Dose of 400 mg/kg of Aloe vera leaf extract was chosen for chronic administration. Oxidative stress parameters viz. malondialdehyde (MDA) and reduced glutathione (GSH) were also estimated in brain of kindled animals. In acute study, Aloe vera leaf (extract) powder in a dose-dependent manner significantly decreased duration of tonic hind limb extension in maximal electroshock seizure model, increased seizure threshold current in increasing current electroshock seizure model, and increased latency to onset and decreased duration of clonic convulsion in pentylenetetrazole (PTZ) model as compared with control group. In chronic study, Aloe vera leaf (extract) powder prevented progression of kindling in PTZ-kindled mice. Aloe vera leaf (extract) powder 400 mg/kg p.o. also reduced brain levels of MDA and increased GSH levels as compared to the PTZ-kindled non-treated group. The results of study showed that Aloe vera leaf (extract) powder possessed significant anticonvulsant and anti-oxidant activity. © 2013 Royal Pharmaceutical Society.

  18. Supercritical Carbon Dioxide extraction of Aloe Emodin and Barbaloin from Aloe Vera L. leaves and their in-vitro cytotoxic activity

    International Nuclear Information System (INIS)

    Kabbash, A.; El-Soud, K.A.; Zalat, E.; Shoeib, N.; Yagi, A.

    2008-01-01

    Aloe emodin and barbaloin, isolated as the active principles of the medicinal plant Aloe vera L., were extracted by supercritical fluid extraction (SFE) and analyzed by high performance liquid chromatography (HPLC). With optimized operating conditions for SFE, aloe emodin and barbaloin were quantitatively extracted from A. Vera leaves within 20 minutes at a flow rate of 0.3 ml/min, temperature and pressure at 40C and 3200 Psi respectively with the addition of 1 ml of methanol as a modifier. Separation of aloe emodin and barbaloin, in a pure form, from the SFE extract was achieved using a semi-preparative column. The cytotoxic activity of both aloe emodin and barbaloin were evaluated using the in-vitro MTT colorimetric assay. Aloe emodin showed a cytotoxic activity on two human colon cancer cells lines (DLD-1 and HD-29) with IC 8.94 and 10.78 M respectively, while barbaloin had no effect. (author)

  19. Antioxidant properties of Sambucus nigra flower extracts.

    OpenAIRE

    Štěpánová, Zuzana

    2013-01-01

    1. Abstract Purpose of this thesis was to prove and determine antioxidant activity of flower extracts from black elder (Sambucus nigra L.) and compile summary of their content substances and effects. Sambuci flos is often used in natural therapy and food industry. The main content substances are flavonoids, phenolic acids, triterpenoids, further are contained in flowers sterols, mucilage, essential oils, tannins. Flavonoids are the most significant, specifically rutin and kvercetin, because t...

  20. DNA degradation by aqueous extract of Aloe vera in the presence of copper ions.

    Science.gov (United States)

    Naqvi, Shoa; Ullah, M F; Hadi, S M

    2010-06-01

    The plant Aloe vera has long been used in medicine, as dietary supplements and for cosmetic purposes. Aloe vera extracts are a rich source of polyphenols, such as aloin and aloe emodin and have shown a wide range of pharmacological properties, including anti-inflammatory and anti-cancer properties. The bioactive component aloe emodin has been reported to induce apoptosis in various cancer cell lines. Many of the biological activities of Aloe vera have been attributed to its antioxidant properties. However, most plant-derived polyphenols that are also present in Aloe vera may exhibit pro-oxidant properties either alone or in the presence of transition metals, such as copper. Previous reports from this laboratory have implicated the pro-oxidant action as one of the mechanisms for their anti-cancer properties. In the present paper, we show that aqueous extract of Aloe vera is also able to cause DNA degradation in the presence of copper ions. Further, the extract is also able to reduce Cu(II) to Cu(I) and generate reactive oxygen species, such as superoxide anion and hydroxyl radicals in a dose-dependent manner, which correlates with ability of the extract to cause DNA breakage. Thus, the study shows that in addition to antioxidant activity, Aloe vera extract also possess pro-oxidant properties, leading to oxidative DNA breakage.

  1. Effects of Aloe vera Extract on the Haemostatic Mechanism of Albino ...

    African Journals Online (AJOL)

    Plants and plant extracts have been found very useful for purposes of treatment of diseases among other benefits. Aloe vera is one such plants, whose effects on the haemostatic mechanism of albino wistar rats were studied. Results showed that Aloe vera did not increase the haemoglobin concentration, and packed cell ...

  2. Anti-inflammatory and Analgesic Effects of Aqueous Extract of Aloe ...

    African Journals Online (AJOL)

    ... 57.59% and 68.06% inhibition respectively. The present study showed that the aqueous extract of Aloe barbadensis has anti-inflammatory and analgesic activities that could be mediated via modulators of pain and inflammation or through central activity. Keywords: Aloe barbadensis; anti-inflammatory; analgesic activity ...

  3. Ultraviolet light absorption of an ophthalmic formulation with Aloe extracts.

    Science.gov (United States)

    Ji, Yimei; Jia, Jicheng

    2009-09-01

    Aloin and polysaccharide present in extracts of Aloe arborescens Miller were formulated into a binary solution to protect eyes from bacterial infection and ultraviolet radiation (UVR). The UVR absorption spectrum was recorded from 190 to 440 nm using a UV spectrometer. The physical properties of the product were examined in terms of its appearance, odor, pH, viscosity, density, refractive index, and stability. The binary solution exhibited three absorption peaks in the UVA, B and C regions, respectively. Such UV absorption capability was attributed to the phenolic chromophores pertaining to aloin. The present study suggested that the formulated binary solution has potential application as an UV absorption agent with built-in antimicrobial activity.

  4. Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study

    Science.gov (United States)

    Rezazadeh, Fahimeh; Moshaverinia, Maryam; Motamedifar, Mohammad; Alyaseri, Montazer

    2016-01-01

    Statement of the Problem Herpes simplex virus (HSV) infection is one of the most common and debilitating oral diseases; yet, there is no standard topical treatment to control it. The extract of Aloe vera leaves has been previously reported to have anti-inflammatory, antibacterial, and also antiviral effects. There is no data on anti-Herpes simplex virus type 1 (HSV-1) activity of Aloe vera gel. Purpose This study aimed to evaluate the anti-HSV-1 activity of Aloe vera gel in Vero cell line. Materials and Method In this study, gel extraction and cytotoxicity of various increasing concentrations of Aloe vera gel (0.2, 0.5, 1, 2, and 5%) was evaluated in Dulbecco’s Modified Eagle Medium (DMEM) containing 2% fetal bovine serum (FBS). Having been washed with phosphate buffered saline, 50 plaque-forming units (PFU) of HSV-1 was added to each well. After 1 hour of incubation at 37°C, cell monolayers in 24 well plates were exposed to different increasing concentrations of Aloe vera gel. The anti-HSV-1 activity of Aloe vera gel in different concentrations was assessed by plaque reduction assays. Data were analyzed by using One-way ANOVA. Results The cytotoxicity assay showed that Aloe vera in prearranged concentrations was cell-compatible. The inhibitory effect of various concentrations of Aloe vera was observed one hour after the Vero cell was infected with HSV-1. However, there was no significant difference between two serial concentrations (p> 0.05). One-way ANOVA also revealed no significant difference between the groups. The findings indicated a dose-dependent antiviral effect of Aloe vera. Conclusion The findings showed significant inhibitory effect of 0.2-5% Aloe vera gel on HSV-1 growth in Vero cell line. Therefore, this gel could be a useful topical treatment for oral HSV-1 infections without any significant toxicity. PMID:26966709

  5. Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study.

    Science.gov (United States)

    Rezazadeh, Fahimeh; Moshaverinia, Maryam; Motamedifar, Mohammad; Alyaseri, Montazer

    2016-03-01

    Herpes simplex virus (HSV) infection is one of the most common and debilitating oral diseases; yet, there is no standard topical treatment to control it. The extract of Aloe vera leaves has been previously reported to have anti-inflammatory, antibacterial, and also antiviral effects. There is no data on anti-Herpes simplex virus type 1 (HSV-1) activity of Aloe vera gel. This study aimed to evaluate the anti-HSV-1 activity of Aloe vera gel in Vero cell line. In this study, gel extraction and cytotoxicity of various increasing concentrations of Aloe vera gel (0.2, 0.5, 1, 2, and 5%) was evaluated in Dulbecco's Modified Eagle Medium (DMEM) containing 2% fetal bovine serum (FBS). Having been washed with phosphate buffered saline, 50 plaque-forming units (PFU) of HSV-1 was added to each well. After 1 hour of incubation at 37°C, cell monolayers in 24 well plates were exposed to different increasing concentrations of Aloe vera gel. The anti-HSV-1 activity of Aloe vera gel in different concentrations was assessed by plaque reduction assays. Data were analyzed by using One-way ANOVA. The cytotoxicity assay showed that Aloe vera in prearranged concentrations was cell-compatible. The inhibitory effect of various concentrations of Aloe vera was observed one hour after the Vero cell was infected with HSV-1. However, there was no significant difference between two serial concentrations (p> 0.05). One-way ANOVA also revealed no significant difference between the groups. The findings indicated a dose-dependent antiviral effect of Aloe vera. The findings showed significant inhibitory effect of 0.2-5% Aloe vera gel on HSV-1 growth in Vero cell line. Therefore, this gel could be a useful topical treatment for oral HSV-1 infections without any significant toxicity.

  6. Symbiotics and Aloe vera and Symphytum officinale extracts in broiler feed

    Directory of Open Access Journals (Sweden)

    Paula Rodrigues Oliveira

    2016-09-01

    Full Text Available This study aimed to test the effects of dietary Aloe vera and Symphytum officinale extracts added separately or in combination with symbiotics on the performance, nutrient utilization, serum biochemical parameters, biometrics, and intestinal histomorfometry of broilers. The experiment had a randomized block design with five treatments and six replicates of ten broilers each. Treatments were as follows: negative control and positive control (diet without and with antibiotic, respectively; 0.2% Aloe vera (AV; 0.2% Symphytum officinale (S; 0.2% functional supplement, composed of symbiotics fermented in Aloe vera and comfrey plant extracts (S+PE. At seven days of age, FI of birds fed the Aloe vera extracts diets were lower than that observed for birds consuming the diet with Symphytum officinale extract and S+PE. Broiler performance remained unaffected by treatments at others ages evaluated. At 10 to 14 days of age the lowest ADCDM ADCCP was shown in group feed NC. The highest ADCCP was observed in PC control group and in diets supplemented with Aloe vera and S+PE. Serum levels of cholesterol, triglycerides, and phosphorus were affected by addition of extracts at seven, 21, and 35 days of age. The longest duodenal villi were observed in broilers fed S+PE diets at seven days of age. Aloe vera and Symphytum officinale extracts and symbiotics can be used in broiler diets as an alternative to growth-promoting antibiotics.

  7. Effect of pomegranate and aloe vera extract on streptococcus mutans: An in vitro study

    Directory of Open Access Journals (Sweden)

    Priya Subramaniam

    2012-01-01

    Full Text Available Introduction: The aim of the present in vitro study was to evaluate and compare the antibacterial effect of pomegranate and aloe vera extracts on Streptococcus mutans. Materials and Methods: Hydroalcoholic extracts of pulp from both Punica granatum (pomegranate and Aloe barbadensis miller (aloe vera were prepared to concentrations of 5, 25, 50 and 100%. Pure sorbitol powder dissolved in distilled water was taken as the negative control. Streptococcus mutans (S mutans was isolated from saliva by inoculation on to Mitus Salivarius Bacitracin (MSB agar, which was then streaked onto agar plates containing Brain Heart Infusion. In each petridish, wells were prepared and using a sterile micropipette, 125μl of the specific concentration of the extract (pomegranate/ aloe vera/ sorbitol was deposited in each well. This was done in triplicate for each concentration of the extracts. The effect of different concentrations of the extracts on S mutans was observed and the data was subjected to statistical analysis. Results: Pomegranate extract showed significantly higher inhibitory effect on S mutans at all concentrations (P≤0.05. On comparison of all three extracts at different concentrations, a significant difference (P≤0.05 was observed only at 50 and 100% concentrations. The inhibitory effect of pomegranate extract was significantly different when compared to aloe vera and sorbitol extracts. (P≤0.01. Discussion: Pomegranate extract has a significant antibacterial effect on S mutans at all concentrations.

  8. SYNTHESIS AND CHARACTERIZATION OF BIODEGRDABLE PLASTIC FROM CASAVA STARCH AND ALOE VERA EXTRACT WITH GLYCEROL PLASTICIZER

    Directory of Open Access Journals (Sweden)

    Mery Apriyani

    2016-05-01

    Full Text Available Synthesis and characterizations of Biodegradable Plastic made of Cassava Waste Starch, glycerol, acetic acid and Aloe vera extract has done. The aims of this research are to study the influence of addition of aloe vera extract in plastics mechanics properties, water vapor transmission rate and biodegradation. There are five main steps in this research, extraction of aloe vera, cassava starch preparation from cassava waste, preparations, characterization and biodegradability study of biodegradable plastic. The addition variations of aloe vera extract that used in this research are 0.01; 0.03; 0.05; 0.07 and 0.14 grams. Results showed that the addition of aloe Vera tends to increased biodegrable plastic thickness to 0.01 mm and elongation to 32.07%. However, biodegradable plastic tensile strength tends to decreased to 23.95 Mpa. Optimum tensile strength is 3.90 Mpa and elongation is 34.43%. Optimum water vapor transmission rate is 2.40 g/m2hours. Biodegradation study of biodegradable plastic showed that addition of aloe vera extract doesn’t significantly influence in plastic degradations.

  9. A biochemical and cellular approach to explore the antiproliferative and prodifferentiative activity of Aloe arborescens leaf extract.

    Science.gov (United States)

    Di Luccia, Blanda; Manzo, Nicola; Vivo, Maria; Galano, Eugenio; Amoresano, Angela; Crescenzi, Elvira; Pollice, Alessandra; Tudisco, Raffaella; Infascelli, Federico; Calabrò, Viola

    2013-12-01

    Aloe arborescens Miller, belonging to the Aloe genus (Liliaceae family), is one of the main varieties of Aloe used worldwide. Although less characterized than the commonest Aloe vera, Aloe arborescens is known to be richer in beneficial phytotherapeutic, anticancer, and radio-protective properties. It is commonly used as a pharmaceutical ingredient for its effect in burn treatment and ability to increase skin wound healing properties. However, very few studies have addressed the biological effects of Aloe at molecular level. The aim of the research is to provide evidences for the antiproliferative properties of Aloe arborescens crude leaf extract using an integrated proteomic and cellular biological approach. We analysed the composition of an Aloe arborescens leaf extract by gas chromatography-mass spectrometry analysis. We found it rich in Aloe-emodin, a hydroxylanthraquinone with known antitumoral activity and in several compounds with anti-oxidant properties. Accordingly, we show that the Aloe extract has antiproliferative effects on several human transformed cell lines and exhibits prodifferentiative effects on both primary and immortalized human keratinocyte. Proteomic analysis of whole cell extracts revealed the presence of proteins with a strong antiproliferative and antimicrobial activity specifically induced in human keratinocytes by Aloe treatment supporting its application as a therapeutical agent. Copyright © 2013 John Wiley & Sons, Ltd.

  10. Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Cho, Jae Young; Chung, Byung Yeoup [Chonbuk National University, Jeonju (Korea, Republic of)

    2011-10-15

    The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, {beta}-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

  11. Reproductive potential of male catfish treated with gel extract of Aloe ...

    African Journals Online (AJOL)

    The reproductive potential of male catfish treated with gel extract of Aloe vera plant was studied using twelve male fish weighing 500-560g.The fish were divided into 3 groups; A, B and C with four fish in each group. Group A was treated with 2% Aloe vera gel while group B was treated with 3% and Group C the control was ...

  12. Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

    International Nuclear Information System (INIS)

    Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun; Cho, Jae Young; Chung, Byung Yeoup

    2011-01-01

    The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, β-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

  13. Holistic Healing Through Herbs: Effectiveness of Aloe Vera on Post Extraction Socket Healing.

    Science.gov (United States)

    Nimma, Vijaya Lakshmi; Talla, Harsha Vardhan; Bairi, Jaya Krishna; Gopaldas, Madhulatha; Bathula, Haritha; Vangdoth, Sandeep

    2017-03-01

    Advances in the field of alternative medicine has encouraged the use of various natural products for multiple uses in the field of dentistry for treatment of various oral diseases. A natural herbal product is Aloe vera, which has number of benefits with no reported side effects and gaining considerable importance in clinical research. The aim of this cross-sectional randomized interventional study was to evaluate the effectiveness of Aloe vera in the healing of post extraction sockets. A sample size of 40 patients undergoing atraumatic extraction was divided into two groups. Group A-patients were prescribed only analgesics and followed for seven consecutive days and socket healing assessment was done. Group B-Patients were given Aloe vera soaked gel foams, followed up to the third and seventh day by two observers and the socket healing was assessed using the standardized index by Landry, Turnbull and Howley. In addition to healing the common complaint associated with extraction wound, the pain was assessed using numerical rating scale. Data obtained was statistically analysed using Mann-whitney U test, Wilcoxon Signed ranks test and Spearman's rank correlation method. Control group on the third and the seventh day showed healing of 60% and 70% respectively and the Aloe vera group showed a better result having a healing potential of 70% on the third day and 90% on the seventh day which was statistically significant with a (p-valueAloe vera group showed a significant decrease in pain after two hours on the day of extraction followed by second, third and seventh day which was statistically significant (p-valueAloe vera has been proved to have a unique property that is implicated in better healing than other group without any side effects. Aloe vera is economical, effective, powerful nutritional supplement and antioxidant that protects and promotes wound healing.

  14. Use of Random Amplified Polymorphic DNA (RAPD) Technique to Study the Genetic Diversity of Eight Aloe Species.

    Science.gov (United States)

    Ezzat, Shahira M; El Sayed, Abeer M; Salama, Maha M

    2016-10-01

    The genus Aloe comprises over 400 species of flowering succulent plants. Aloe leaves are used in the treatment of asthma, gastrointestinal ulcers, cardiovascular disease, tumors, burns, and diabetes. They are rich in anthraquinones, such as aloin, aloe-emodin, chrysophanol, aloinoside A, and aloinoside B. The various species of Aloe show chemical and morphological similarity and diversity, which depend on the genotype and environmental conditions. In a continuity to our interest in the genus Aloe , this study targets the authentication of eight different Aloe species, Aloe vera (A 1 ) , Aloe arborescens (A 2 ) , Aloe eru (A 3 ) , Aloe grandidentata (A 4 ) , Aloe perfoliata (A 5 ), Aloe brevifolia (A 6 ) , Aloe saponaria (A 7 ), and Aloe ferox (A 8 ), grown in Egypt by using the technique of random amplified polymorphic DNA. Twelve decamer primers were screened in amplification with genomic DNA extracted from all species, of which five primers yielded species-specific reproducible bands. Out of 156 loci detected, the polymorphic, monomorphic, and unique loci were 107, 26, and 23, respectively. Based on a dendrogram and similarity matrix, the eight Aloe species were differentiated from each other and showed more divergence. Aloe species prevailed similarity coefficients of 54-70 % by which they could be classified into three major groups. Thus, this technique may contribute to the identification of these Aloe species that have great morphological similarity in the Egyptian local markets. Georg Thieme Verlag KG Stuttgart · New York.

  15. Extracts from Aloe ferox and Withania somnifera inhibit Candida ...

    African Journals Online (AJOL)

    SERVER

    2008-01-04

    Jan 4, 2008 ... Herbal medicines play a vital role in the treatment of sexually transmitted infections (STIs), especially in the remote areas of South Africa where clinics and hospitals are sparsely located. Aloe ferox and. Withania somnifera are among the southern African plants commonly used for the treatment of (STIs).

  16. Dye-sensitized solar cells using Aloe Vera and Cladode of Cactus extracts as natural sensitizers

    Science.gov (United States)

    Ganta, D.; Jara, J.; Villanueva, R.

    2017-07-01

    The purpose of this study is to develop dye-sensitized solar cells (DSSCs) from natural plant-based dyes, extracted from the Cladode (nopal) of the Thornless Prickly Pear Cactus (Opuntia ficus-indica), the gel of Aloe Vera (Aloe barbadensis miller), and the combination of Cladode and Aloe Vera extracts on side-by-side configuration. Optical properties were analyzed using UV-Vis Absorption and Fourier Transform Infrared Spectroscopy. Open circuit voltages (Voc) varied from 0.440 to 0.676 V, fill factors (FF) were greater than 40%, short-circuit photocurrent densities (Jsc) ranged from 0.112 to 0.290 mA/cm2 and highest conversion efficiency of 0.740% was reported for the Cladode DSSC.

  17. The effects of crude aqueous and alcohol extracts of Aloe vera on growth and abdominal viscera of suckling rats.

    Science.gov (United States)

    Beya, Wabeya; Davidson, Bruce; Erlwanger, Kennedy H

    2012-01-01

    The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day old Sprague-Dawley rats were gavaged with aqueous or alcohol extracts of Aloe vera (low dose 50mg. kg⁻¹ or high dose 500mg. kg⁻¹) daily for eight days. All data were expressed as mean ± SD and analyzed by one way ANOVA. Pups receiving high doses of either extract had a significantly higher body mass gain than the group receiving lower dose (p Aloe vera extracts resulted in growth promotion, enhanced hepatic storage of metabolic substrates, increased ALP possibly in relation to bone growth and caused hypertrophy of the caecum of neonatal rats. These effects need to be explored further to enhance animal production and health.

  18. In vitro activity of Allium sativum and Aloe vera extract against ...

    African Journals Online (AJOL)

    The rapid increase of antibiotic resistance needs to be taken as a threat to both animals and human being. In this study the arbitrary concentration of 25%, 50% and 75% of individual and combined Allium sativum and Aloe vera were tested against Salmonella Gallinarum. The antimicrobials were extracted using aqueous ...

  19. Effect of Aloe Vera Gel Extract on Cadmium-Induced High Blood ...

    African Journals Online (AJOL)

    treatment with, 300mg/kg and 600mg/kg of prevented cadmium-induced hypertension. Treatment of cadmium induced hypertensive rats with 300mg/kg and 600mg/kg of Aloe vera gel extract, significantly lowered blood pressure (p< 0.05) when ...

  20. Anti-Sickling Potential of Aloe Vea Extract Iii: Gas Chromatography ...

    African Journals Online (AJOL)

    The neutral metabolite obtained from ethanol extract of Aloe vera was characterized using the Gas Chromatography/Mass Spectroscopy. The results showed that the oil contained five different compounds, which included hexadecanoic acid, ethyl ester; phytol; 2-methyloctadeca-3,13-diene-1-ol; cotadeca-9,12,15-trienoic ...

  1. Gamma irradiation response of RBC in presence of Aloe vera leaf extract

    International Nuclear Information System (INIS)

    Rane, Charusheela C.; Patil, Shilpa M.; Kulkarni, Satish G.

    2004-01-01

    Full text: The components from fleshy leaves of Aloe vera have wide range of utility in treating human aliments owing to their medicinal property including antioxidant characteristic. During gamma irradiation, attack by free radicals is the prominent cause of damage to cell membrane. This action is modified to varied extent by several components from plants. A radio modulation effect of different dilutions of Aloe vera leave extract against gamma radiation has been evaluated by measuring percent haemolysis. The result obtained will be discussed to narrate influencing role of various factors in modulation

  2. Mechanism of Aloe Vera extract protection against UVA: shelter of lysosomal membrane avoids photodamage.

    Science.gov (United States)

    Rodrigues, Daniela; Viotto, Ana Cláudia; Checchia, Robert; Gomide, Andreza; Severino, Divinomar; Itri, Rosangela; Baptista, Maurício S; Martins, Waleska Kerllen

    2016-03-01

    The premature aging (photoaging) of skin characterized by wrinkles, a leathery texture and mottled pigmentation is a well-documented consequence of exposure to sunlight. UVA is an important risk factor for human cancer also associated with induction of inflammation, immunosuppression, photoaging and melanogenesis. Although herbal compounds are commonly used as photoprotectants against the harmful effects of UVA, the mechanisms involved in the photodamage are not precisely known. In this study, we investigated the effects of Aloe Vera (Aloe barbadensis mil) on the protection against UVA-modulated cell killing of HaCaT keratinocytes. Aloe Vera exhibited the remarkable ability of reducing both in vitro and in vivo photodamage, even though it does not have anti-radical properties. Interestingly, the protection conferred by Aloe Vera was associated with the maintenance of membrane integrity in both mimetic membranes and intracellular organelles. The increased lysosomal stability led to a decrease in lipofuscinogenesis and cell death. This study explains why Aloe Vera extracts offer protection against photodamage at a cellular level in both the UV and visible spectra, leading to its beneficial use as a supplement in protective dermatological formulations.

  3. Radioprotective effects of Aloe vera leaf extract on skin of Swiss mice after gamma irradiation

    International Nuclear Information System (INIS)

    Gehlot, Prashasnika; Saini, M.R.

    2004-01-01

    Full text: Biological effects of radiation are detrimental to life. Skin being a cell-renewal system is one of the best organ for studying radiation induced effects and their modulation by antioxidants. An attempt has been made to evaluate radioprotective efficacy of Aloe vera leaf extract on skin in Swiss mice (1g/kg body wt/day). The mice selected from inbreed colony were divided into two groups. The first group was given Aloe vera extract orally for 15th consecutive days and served as experimental group while the other group received DDW (vol. equal to Aloe extract) to serve as control group. On the 15th day, after 30 min of above treatment animals of both the groups were exposed to 2 Gy gamma irradiation and autopsied on 6h 1, 3, 7, 14 and 21 days. DNA as well as total protein decreases in control group as compared to the normal value. Surprisingly, in experimental group, DNA and protein increases in comparison to the control group. Thus, Aloe vera were found to have positive influence against radiation induced alterations on skin of Swiss albino mice

  4. Evaluation of free radical scavenging and radioprotective efficacy of Aloe vera extract

    International Nuclear Information System (INIS)

    Chakrawarti, Aruna; Purohit, R.K.; Agarwal, Manisha; Ranga, Anita; Saran, Leena

    2012-01-01

    Radiation induced damage and lethality to the normal tissues can be partially reduced by the use of radio-protectors that lower down the damaging effects of radiation. In the present study radio-protective effect of Aloe vera extract which is rich in vitamins. It is basically cellular regenerator and therefore acts, with very positive results in many conditions. It is reported to have antioxidant, anti carcinogenic, antimicrobial and immunity stimulants properties. For the present experiment, healthy Swiss albino mice (6-8 weeks old) were selected and maintained under standard conditions of temperature and light. Aloe vera juice was fed orally at the dose rate of 0.01 m1/animal/day. The animals were divided into seven groups according to the treatment given to them i.e. given cadmium chloride solution as drinking water (group-II) or exposed to 5.0 Gy gamma radiations (group-III) or combined treatment of radiation and cadmium chloride (group-IV). The alterations in the biochemical parameters of all these group were compared with that of sham irradiated animals (group-I). The animals of experimental groups were given Aloe vera seven days prior to radiation or cadmium chloride treatment (group-V, VI and VII) respectively. All biochemical parameters of the control groups were compared with the respective experimental groups. An increase in the value of total proteins, glycogen, acid and alkaline phosphatase activity and RNA was observed up to day-14 in the non drug treated group and day-7 in the Aloe vera groups. Thereafter value declined up to day-28 without reaching to the normal. Whereas the value of cholesterol and DNA showed a decreasing trend up to day-14 in non drug treated groups and day-7 in Aloe vera treated groups. Thus it showed that cadmium and radiation produced toxic effect on kidney and Aloe vera minimize these effects. Protection offered by Aloe vera may be due to the scavenging or oxidizing free radicals. Thus it can be concluded that Aloe vera may

  5. Aphrodisiac potentials of the ethanol extract of Aloe barbadensis Mill. root in male Wistar rats

    OpenAIRE

    Erhabor, Joseph O.; Idu, MacDonald

    2017-01-01

    Background Aloe barbadensis (AB) is a short stemmed succulent medicinal herb that is being used by locals in Nigeria to enhance libido. Therefore this study evaluates the aphrodisiac potential and acute toxicological effect of A. barbadensis (AB) root in male Wistar rats. Methods Aphrodisiac potential was determined following the oral administration of graded doses (100, 200 and 400 mg/kg) of ethanol extract of A. barbadensis root. Sildenafil citrate (Viagra) and distilled water served as pos...

  6. A comparison of the leaf gel extracts of Aloe ferox and Aloe vera in the topical treatment of atopic dermatitis in Balb/c mice.

    Science.gov (United States)

    Finberg, M J; Muntingh, G L; van Rensburg, C E J

    2015-12-01

    Aloe vera gel is widely used in the treatment of an array of disturbances, especially skin disorders. The wound-healing effects have been attributed to its moisturizing and anti-inflammatory effects as well as its beneficial effect on the maturation of collagen. The aim of the present study is to compare the effects of topically applied extracts of Aloe ferox with that of Aloe vera on the symptoms as well as IgE levels of a mouse model of atopic dermatitis (AD). Mice were sensitized and challenged with 2,4-dinitrochlorobenzene and treated afterwards for 10 consecutive days with the gels of either A. ferox or A. vera applied topically to the affected areas. A placebo gel was used for the control mice. Blood was collected at the beginning and end of the treatment period to measure serum IgE levels. Although the gels of both the Aloe species inhibited the cutaneous inflammatory response as well as serum IgE levels in the rats, the extracts of A. ferox were superior to that of A. vera in reducing IgE levels. The gels of A. ferox and A. vera, applied topically, may be a safe and useful alternative to antihistamines and topical corticosteroids, for the treatment of patients suffering from recurring chronic AD.

  7. Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis.

    Science.gov (United States)

    Dotta, G; Ledic-Neto, J; Gonçalves, E L T; Brum, A; Maraschin, M; Martins, M L

    2015-05-01

    This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.

  8. Clear Evidence of Carcinogenic Activity by a Whole-Leaf Extract of Aloe barbadensis Miller (Aloe vera) in F344/N Rats

    Science.gov (United States)

    Boudreau, Mary D.

    2013-01-01

    Aloe barbadensis Miller (Aloe vera) is an herbal remedy promoted to treat a variety of illnesses; however, only limited data are available on the safety of this dietary supplement. Drinking water exposure of F344/N rats and B6C3F1 mice to an Aloe vera whole-leaf extract (1, 2, and 3%) for 13 weeks resulted in goblet cell hyperplasia of the large intestine in both species. Based upon this observation, 2-year drinking water studies were conducted to assess the carcinogenic potential of an Aloe vera whole-leaf extract when administered to F344/N rats (48 per sex per group) at 0.5, 1, and 1.5%, and B6C3F1 mice (48 per sex per group) at 1, 2, and 3%. Compared with controls, survival was decreased in the 1.5% dose group of female rats. Treatment-related neoplasms and nonneoplastic lesions in both species were confined primarily to the large intestine. Incidences of adenomas and/or carcinomas of the ileo-cecal and cecal-colic junction, cecum, and ascending and transverse colon were significantly higher than controls in male and female rats in the 1 and 1.5% dose groups. There were no neoplasms of the large intestine in mice or in the 0 or 0.5% dose groups of rats. Increased incidences of mucosa hyperplasia of the large intestine were observed in F344/N rats, and increased incidences of goblet cell hyperplasia of the large intestine occurred in B6C3F1 mice. These results indicate that Aloe vera whole-leaf extract is an intestinal irritant in F344/N rats and B6C3F1 mice and a carcinogen of the large intestine in F344/N rats. PMID:22968693

  9. Extracts from Aloe ferox and Withania somnifera inhibit Candida ...

    African Journals Online (AJOL)

    Withania somnifera are among the southern African plants commonly used for the treatment of (STIs). This paper reports on the in vitro antimicrobial activities of water and methanol extracts from the two plants on Neisseria gonorrhoea and Candida albicans, common causes of STIs in rural South Africa. Extracts from both ...

  10. Toxicology and carcinogenesis studies of a nondecolorized [corrected] whole leaf extract of Aloe barbadensis Miller (Aloe vera) in F344/N rats and B6C3F1 mice (drinking water study).

    Science.gov (United States)

    Boudreau, M D; Beland, F A; Nichols, J A; Pogribna, M

    2013-08-01

    Extracts from the leaves of the Aloe vera plant (Aloe barbadensis Miller) have long been used as herbal remedies and are also now promoted as a dietary supplement, in liquid tonics, powders or tablets, as a laxative and to prevent a variety of illnesses. We studied the effects of Aloe vera extract on rats and mice to identify potential toxic or cancer-related hazards. We gave solutions of nondecolorized extracts of Aloe vera leaves in the drinking water to groups of rats and mice for 2 years. Groups of 48 rats received solutions containing 0.5%, 1% or 1.5% of Aloe vera extract in the drinking water, and groups of mice received solutions containing 1%, 2%, or 3% of Aloe vera extract. Similar groups of animals were given plain drinking water and served as the control groups. At the end of the study tissues from more than 40 sites were examined for every animal. In all groups of rats and mice receiving the Aloe vera extract, the rates of hyperplasia in the large intestine were markedly increased compared to the control animals. There were also increases in hyperplasia in the small intestine in rats receiving the Aloe vera extract, increases in hyperplasia of the stomach in male and female rats and female mice receiving the Aloe vera extract, and increases in hyperplasia of the mesenteric lymph nodes in male and female rats and male mice receiving the Aloe vera extract. In addition, cancers of the large intestine occurred in male and female rats given the Aloe vera extract, though none had been seen in the control groups of rats for this and other studies at this laboratory. We conclude that nondecolorized Aloe vera caused cancers of the large intestine in male and female rats and also caused hyperplasia of the large intestine, small intestine, stomach, and lymph nodes in male and female rats. Aloe vera extract also caused hyperplasia of the large intestine in male and female mice and hyperplasia of the mesenteric lymph node in male mice and hyperplasia of the stomach

  11. Antifungal activity evaluation of Aloe arborescens dry extract against trichosporon genus yeasts

    Directory of Open Access Journals (Sweden)

    João Ricardo Bueno de Morais Borba

    2014-10-01

    Full Text Available The objective of this study was to investigate the antifungal activity of Aloe arborescens dry extract against Trichosporon genus yeast species. Extraction was carried out by means of a longitudinal incision in fresh leaves, which were collected on a vat, and the total volume was frozen and subsequently lyophilized. Then, 40 mg of the dry extract was dissolved in DMSO by gentle inversion in order to obtain a solution whose concentration was 4000 µg mL-1. This solution became limpid and slightly yellowish because the pigment of the latex was attenuated. It was performed serial dilutions from 2,000 to 15.625 µg mL-1 with RPMI-1640 broth. There was already no pigment in the first dilution of 2000 μg mL-1. It was analyzed fifteen strains of Trichosporon spp., and Candida albicans ATCC 10231 was used as control strain. We carried out the reading of microplates in the ELISA reader device at a wavelength of 530 nm, after incubation for 24 and 48 hours, and it was determinated the Minimum Inhibitory Concentration (MIC. The MIC50 value obtained for all Trichosporon species and for C. albicans was 500 µg mL-1. As a result, we concluded that Aloe arborescens dry extract has antifungal activity against Trichosporon yeasts.

  12. Anti-hyperglycemic effect of Aloe vera peel extract on blood sugar level of alloxan-induced Wistar rats

    Science.gov (United States)

    Peniati, E.; Setiadi, E.; Susanti, R.; Iswari, R. S.

    2018-03-01

    Aloe vera peel contains flavonoids, alkaloids, tannins, saponins, and sterols as its secondary metabolites. This research explores the effect of Aloe vera peel extract on blood glucose levels of alloxan-induced Wistar rats in a laboratory experimental scale. Blood glucose examination was performed by using GOD-PAP method. Twenty five 2 months old-white rat (Rattus norvegicus) male wistar strain weigh 150-200 grams body weight, and in healthy condition, was randomly divided into five groups. Those five groups were negative control group (K-), positive control group (K+), treatment group 1 (P1), treatment group 2 (P 2), and treatment group 3 (P 3). Each group was fed by standard diet and ad-libitum drinking. Treatments were given for 28 days. On the day 29, blood glucose level of all groups were analyzed. The results showed that the highest blood glucose levels in control group rat were positive (191.2 mg/dl). Aloe vera extract was able to decrease blood sugar level up to 104,6mg/dl in P3 group treatment rats (served Aloe vera extract 350 mg/kg BW/day). It comes to the conclusion that giving Aloe vera peel extract for 28 days decreases blood sugar level of hyperglycemic rat.

  13. Antioxidant activities of extracts from areca (Areca catectu L.) flower ...

    African Journals Online (AJOL)

    The antioxidant activities of areca ( Areca catectu L.) flower, husk and seed extracts were evaluated using 3 complementary in vitro assays, inhibition of DPPH (1,1-diphenyl-2- picrylhydrazyl) radicals, inhibition of hydroxyl radicals and reducing power system. The EC50 values were calculated for all the methods in order to ...

  14. Impact of 50% ethanolic extract of Calendula officinalis (flower) on ...

    African Journals Online (AJOL)

    Oral administration to male rats of 200mg kg-1 body weight of an extract of Calendula officinalis flowers every day for 60 days did not cause loss of body weight, but decreased significantly the weight of the testis, epididymis, seminal vesicle and ventral prostate. Sperm motility as well as sperm density were reduced ...

  15. Hypoglycemic and Hypolipidemic Effects of Aloe vera Extract Preparations: A Review.

    Science.gov (United States)

    Pothuraju, Ramesh; Sharma, Raj Kumar; Onteru, Suneel Kumar; Singh, Satvinder; Hussain, Shaik Abdul

    2016-02-01

    Obesity is considered to be an epidemic disease, and it is associated with several metabolic disorders. Pharmacological treatments currently available are not effective for prolonged treatment duration. So, people are looking toward new therapeutic approach such as herbal ingredients. Since ancient periods, different herbs have been used for remedy purposes such as anti-obesity, antidiabetes, and antiinflammatory. Among the several herbal ingredients, Aloe vera (Aloe barbadensis Miller) is widely used to curb the metabolic complications. Till date, reports are not available for the side effects of A. vera. Several researchers are used to different solvents such as aqueous solution, alcohol, ethanol, and chloroform for the A. vera extract preparations and studied their hypoglycemic and hypolipidemic effects in animal and human studies. Furthermore, little information was recorded with the active compounds extracted from the A. vera and their anti-obesity and antidiabetic effects in clinical studies. In this review, we made an attempt to compile all the available literature by using different search engines (PubMed, Scopus, and Google Scholar) on the A. vera extract preparations and the possible mechanism of action involved in carbohydrate and lipid metabolism. Copyright © 2015 John Wiley & Sons, Ltd.

  16. Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract.

    Science.gov (United States)

    Chandran, S Prathap; Chaudhary, Minakshi; Pasricha, Renu; Ahmad, Absar; Sastry, Murali

    2006-01-01

    Biogenic gold nanotriangles and spherical silver nanoparticles were synthesized by a simple procedure using Aloe vera leaf extract as the reducing agent. This procedure offers control over the size of the gold nanotriangle and thereby a handle to tune their optical properties, particularly the position of the longitudinal surface plasmon resonance. The kinetics of gold nanotriangle formation was followed by UV-vis-NIR absorption spectroscopy and transmission electron microscopy (TEM). The effect of reducing agent concentration in the reaction mixture on the yield and size of the gold nanotriangles was studied using transmission electron microscopy. Monitoring the formation of gold nanotriangles as a function of time using TEM reveals that multiply twinned particles (MTPs) play an important role in the formation of gold nanotriangles. It is observed that the slow rate of the reaction along with the shape directing effect of the constituents of the extract are responsible for the formation of single crystalline gold nanotriangles. Reduction of silver ions by Aloe vera extract however, led to the formation of spherical silver nanoparticles of 15.2 nm +/- 4.2 nm size.

  17. Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques.

    Science.gov (United States)

    Dal'Belo, Susi Elaine; Gaspar, Lorena Rigo; Maia Campos, Patrícia Maria Berardo Gonçalves

    2006-11-01

    The polysaccharide-rich composition of Aloe vera extracts (Aloe barbadensis Miller), often used in cosmetic formulations, may impart moisturizing properties to the product. The aim of this study was to evaluate the effect of cosmetic formulations containing different concentrations of freeze-dried Aloe vera extract on skin hydration, after a single and a 1- and 2-week period of application, by using skin bioengineering techniques. Stable formulations containing 5% (w/w) of a trilaureth-4 phosphate-based blend were supplemented with 0.10%, 0.25% or 0.50% (w/w) of freeze-dried Aloe vera extract and applied to the volar forearm of 20 female subjects. Skin conditions in terms of the water content of the stratum corneum and of transepidermal water loss (TEWL) (Corneometer CM 825 and Tewameter TM 210) were analysed before and after a single and 1- and 2-week period of daily application. After a single application, only formulations supplemented with 0.25% and 0.50% (w/w) of Aloe vera extract increased the water content of the stratum corneum, while after the 2-week period application, all formulations containing the extract (0.10%, 0.25% and 0.50%) had the same effect, in both cases as compared with the vehicle. TEWL was not modified after a single and after 1- and 2-week period of application, when compared with the vehicle. Our results show that freeze-dried Aloe vera extract is a natural effective ingredient for improving skin hydration, possibly through a humectant mechanism. Consequently, it may be used in moisturizing cosmetic formulations and also as a complement in the treatment of dry skin.

  18. Short Period Starvation in Rat: The Effect of Aloe Vera Gel Extract on Oxidative Stress Status Ion

    Directory of Open Access Journals (Sweden)

    Laleh SHAHRAKI MOJAHED

    2017-01-01

    Full Text Available The main aim of this experiment was to evaluate the effect of Aloe vera gel extract on oxidative stress status during starvation. For this purpose, twenty-four mature male albino Wistar rats were housed in standard cages. In this study starvation cycle (rats were starved for two days and then were fed for one day was used. This study was performed during short period (20 days. Animals were divided into four experimental groups (six rats in each group: 1 normal control; 2 starved rats+water/ethanol; 3 starved rats+hydro-alcoholic Aloe vera gel extract (100 mg/kg; 4 starved rats+hydro-alcoholic Aloe vera gel extract (200 mg/kg. Blood samples were obtained using cardiac puncture. In blood samples, antioxidant enzymes including superoxide dismutase (SOD, glutathione peroxidase (GPx and catalase (CAT, antioxidant trace elements including copper, zinc and manganese and antioxidant vitamins including vitamin A, vitamin E and vitamin C were measured. Plasma levels of antioxidant enzymes (SOD, GPx and catalase significantly decreased in starved rats+water/ethanol group (P0.05. Plasma levels of vitamin A and E in normal control group had no significant difference with starved rats+water/ethanol and starved rats+hydro-alcoholic Aloe vera gel extract in the 100 and 200 mg/kg dose groups (P>0.05. Plasma level of vitamin C significantly decreased in starved rats+water/ethanol group (P<0.05. Plasma level of vitamin C after treatment with hydro-alcoholic Aloe vera gel extract at doses 100 and 200 mg/kg were significantly increased (P<0.05. Our results shown that short term starvation caused an increase in oxidative stress via impairing of antioxidant defense and Aloe vera treatment is able to improve antioxidative defense induced by starvation.

  19. Rectification of radiation-induced damage in swiss albino mice by aloe vera leaf extracts (AVE)

    International Nuclear Information System (INIS)

    Gehlot, P.; Goyal, P. K.

    2007-01-01

    From the time immemorial man has been exposed to ionizing radiation from the environment in which he lives. Radiation protection concepts and philosophy have been evolving over the past several decades. Materials and Methods: The radioprotective of effect of Aloe vera leaf extract (1000 mg/kg b.wt. orally for 15 consecutive days) has been studied against 6 Gy of gamma radiation in the intestine of Swiss albino mice at various post irradiation intervals viz. 12 hours, 24 hours. and 3, 5, 10, 20 and 30 days. Results: Crypt survival, villus length, apoptic cells, mitotic figures and goblet cells in jejunum were studied after irradiation. Irradiation produced a significant decrease in crypt survival, mitotic figures and villus length; whereas goblet and apoptic cells showed a significant increase from sham irradiated animals. The major changes were observed on day 3 after irradiation. AVE pre-treated irradiated animals resulted in a significant increase in the number of crypt cells, mitotic figures and villus length; whereas the counts of apoptic and goblet cells showed a significant decrease from respective control group at all the autopsy intervals. Irradiated animals resulted in the elevation in lipid peroxidation and a reduction in glutathione activity. On contrary, AVE treatment before irradiation caused a significant depletion in lipid peroxidation and elevation in glutathione activity. Conclusion: The present study suggests the possible radioprotective ability of Aloe vera leaf extract

  20. Prevention of radiation-induced hepatic damage in Swiss albino mice by Aloe Vera leaf extract

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    Gehlot Prashasnika

    2010-01-01

    Full Text Available The radioprotective effect of the Aloe vera leaf extract was studied in Swiss albino mice against radiation-induced changes in the liver. The mice were treated with 1000 mg/kg of body weight orally, once a day for 15 consecutive days, before exposure to a single dose of gamma radiation (6 Gy, half an hour after the last administration. The irradiation of mice caused a significant elevation in lipid peroxidation followed by a decrease in glutathione, acid phosphatase and alkaline phosphatase. The treatment of mice before irradiation elevated the glutathione, acid phosphatase and alkaline phosphatase, and was accompanied by a decline in lipid peroxidation. Recovery and regeneration from radiation damage were faster in pretreated animals than the animals in the irradiation-only group. The data clearly indicate that the Aloe vera leaf extract significantly reduced the deleterious effects of radiation on the liver and it could be a useful agent in reducing the side effects of therapeutic radiation.

  1. [The therapeutic properties of aloe].

    Science.gov (United States)

    Iena, Ia M

    1993-01-01

    Data are reported on the use of aloes in research and folk medicine. Aloes is used in the form of dry juice of aloe leaves, fluid extract, juice, ointments. The author discusses indications and contraindications to the use of aloe. Recipes are given of mixtures with aloe which may be used in domestic conditions for increasing the defensive forces of the body during radiation lesions.

  2. Microwave-assisted fibrous decoration of mPE surface utilizing Aloe vera extract for tissue engineering applications.

    Science.gov (United States)

    Balaji, Arunpandian; Jaganathan, Saravana Kumar; Supriyanto, Eko; Muhamad, Ida Idayu; Khudzari, Ahmad Zahran Md

    2015-01-01

    Developing multifaceted, biocompatible, artificial implants for tissue engineering is a growing field of research. In recent times, several works have been reported about the utilization of biomolecules in combination with synthetic materials to achieve this process. Accordingly, in this study, the ability of an extract obtained from Aloe vera, a commonly used medicinal plant in influencing the biocompatibility of artificial material, is scrutinized using metallocene polyethylene (mPE). The process of coating dense fibrous Aloe vera extract on the surface of mPE was carried out using microwaves. Then, several physicochemical and blood compatibility characterization experiments were performed to disclose the effects of corresponding surface modification. The Fourier transform infrared spectrum showed characteristic vibrations of several active constituents available in Aloe vera and exhibited peak shifts at far infrared regions due to aloe-based mineral deposition. Meanwhile, the contact angle analysis demonstrated a drastic increase in wettability of coated samples, which confirmed the presence of active components on glazed mPE surface. Moreover, the bio-mimic structure of Aloe vera fibers and the influence of microwaves in enhancing the coating characteristics were also meticulously displayed through scanning electron microscopy micrographs and Hirox 3D images. The existence of nanoscale roughness was interpreted through high-resolution profiles obtained from atomic force microscopy. And the extent of variations in irregularities was delineated by measuring average roughness. Aloe vera-induced enrichment in the hemocompatible properties of mPE was established by carrying out in vitro tests such as activated partial thromboplastin time, prothrombin time, platelet adhesion, and hemolysis assay. In conclusion, the Aloe vera-glazed mPE substrate was inferred to attain desirable properties required for multifaceted biomedical implants.

  3. Antioxidant Properties of Crude Extract, Partition Extract, and Fermented Medium of Dendrobium sabin Flower

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    Farahziela Abu

    2017-01-01

    Full Text Available Antioxidant properties of crude extract, partition extract, and fermented medium from Dendrobium sabin (DS flower were investigated. The oven-dried DS flower was extracted using 100% methanol (w/v, 100% ethanol (w/v, and 100% water (w/v. The 100% methanolic crude extract showed the highest total phenolic content (40.33 ± mg GAE/g extract and the best antioxidant properties as shown by DPPH, ABTS, and FRAP assays. A correlation relationship between antioxidant activity and total phenolic content showed that phenolic compounds were the dominant antioxidant components in this flower extract. The microbial fermentation on DS flower medium showed a potential in increasing the phenolic content and DPPH scavenging activity. The TPC of final fermented medium showed approximately 18% increment, while the DPPH of fermented medium increased significantly to approximately 80% at the end of the fermentation. Dendrobium sabin (DS flower showed very good potential properties of antioxidant in crude extract and partition extract as well as better antioxidant activity in the flower fermented medium.

  4. CHEMICAL ANALYSIS OF DENSE-GAS EXTRACTS FROM LIME FLOWERS

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    Demyanenko DV

    2015-04-01

    Full Text Available The purpose of this work was to make qualitative and quantitative analysis of phenolic biologically active substances (BAS in the extracts produced from lime flowers with condensed gases, using method of high-performance liquid chromatography (HPLC. Materials and methods: materials for this study were the extracts obtained by consequent processing of the herbal drug and marcs thereof with various condensed gases: difluorochloromethane (Freon R22, difluoromethane (Freon R32, azeotropic mixture of difluoromethane with pentafluoroethane (Freon 410A and freon-ammonium mixture. Extracts obtained with the latter were subjected to further fractionation by liquidliquid separation into hexane, chloroform, ethyl acetate and aqueous-alcohol phases. Besides, the supercritical СО2 extract, obtained from the herbal drug under rather strong conditions (at temperature 60°С and pressure 400 bar, was studied in our previous research. Presence of phenolic BAS and their quantity in the researched samples were determined by method of HPLC with UVspectrometric detection. Results and discussion: It has been found that Freon R22 extracted trace amounts of rutin from lime flowers – its content was only 0.08% of the total extract weight. On the other hand, Freons R32 and R410А showed good selectivity to moderately polar BAS of lime flowers (derivatives of flavonoids and hydroxycinnamic acids: in particular, the extract obtained with freon R32 contained about 1.3% of the total phenolic substances, and it was the only one of the investigated condensed gases used by us which took the basic flavonoid of lime flowers tiliroside – its content was 0.42% of extract weight. Also Freons R32 and R410А were able to withdraw another compound dominating among phenolic substances in the yielded extracts. Its quantity was rather noticeable – up to 0.87% of extract weight. This substance was not identified by existing database, but its UV-spectrum was similar to those of

  5. Enhanced biocompatibility of ZnS:Mn quantum dots encapsulated with Aloe vera extract for therapeutic applications

    Science.gov (United States)

    Anilkumar, M.; Bindu, K. R.; Sneha Saj, A.; Anila, E. I.

    2016-08-01

    Toxicity of nanoparticles remains to be a major issue in their application to the biomedical field. Aloe vera (AV) is one of the most widely exploited medicinal plants that have a multitude of amazing properties in the field of medicine. Methanol extract of Aloe vera can be used as a novel stabilising agent for quantum dots to reduce toxicity. We report the synthesis, structural characterization, antibacterial activity and cytotoxicity studies of ZnS:Mn quantum dots synthesized by the colloidal precipitation method, using methanol extract of Aloe vera (AVME) as the capping agent. The ZnS:Mn quantum dots capped with AVME exhibit superior performances in biocompatibility and antibacterial activity compared with ZnS:Mn quantum dots without encapsulation. Project supported by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India.

  6. Aloe vera extract as a promising treatment for the quality maintenance of minimally-processed table grapes

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    Giuseppina Rosaria Antonella Alberio

    2015-06-01

    Full Text Available The effect of an edible film obtained from a commercial Aloe vera extract, on the quality maintenance of minimally processed grapes belonging to three different cultivars (Sugar One, Victoria and Black Magic was evaluated by enzymatic (PPO, PME, β-GAL, physicochemical (pH, acidity, °Brix, and sensorial methods. All the analyzed parameters were measured in extracts obtained from minimally processed grapes packaged in ordinary atmosphere and stored at 4 °C for 15 days. Samples dipped into Aloe vera showed significant differences (p≤0.05 compared to untreated ones. The determination of such parameters and the evaluation of consumer acceptability were helpful to determine the effectiveness of the post-harvest treatment with Aloe vera for a storage period of 15 days.

  7. Enhanced biocompatibility of ZnS:Mn quantum dots encapsulated with Aloe vera extract for therapeutic applications

    International Nuclear Information System (INIS)

    Anilkumar, M; Sneha Saj, A; Bindu, K R; Anila, E I

    2016-01-01

    Toxicity of nanoparticles remains to be a major issue in their application to the biomedical field. Aloe vera (AV) is one of the most widely exploited medicinal plants that have a multitude of amazing properties in the field of medicine. Methanol extract of Aloe vera can be used as a novel stabilising agent for quantum dots to reduce toxicity. We report the synthesis, structural characterization, antibacterial activity and cytotoxicity studies of ZnS:Mn quantum dots synthesized by the colloidal precipitation method, using methanol extract of Aloe vera (AVME) as the capping agent. The ZnS:Mn quantum dots capped with AVME exhibit superior performances in biocompatibility and antibacterial activity compared with ZnS:Mn quantum dots without encapsulation. (paper)

  8. Phytochemical Contents and Antioxidant Capacities of Two Aloe greatheadii var. davyana Extracts

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    Du Toit Loots

    2008-09-01

    Full Text Available Aloe greatheadii var. davyana (Asphodelaceae is used among rural South African communities to treat arthritis, skin cancer, burns, eczema, psoriasis, digestive problems, high blood pressure and diabetes, despite very little supporting scientific evidence. Due to increased interest by both the scientific community and industry regarding the medicinal uses of this plant species, we identified, quantified and compared the phytochemical contents and antioxidant capacities of two extracts of A. greatheadii; a leaf gel extract (LGE and a 95 % aqueous ethanol leaf gel extract (ELGE, using various modified extraction procedures, GC-MS and spectrophotometry. Apart from extensively characterizing this medicinal plant with regards to its organic acid, polyphenols/phenolic acid, alcohol, aldehyde, ketone, alkane, pyrimidine, indole, alkaloid, phytosterol, fatty acid and dicarboxylic acid contents and antioxidant capacities, we describe a modified extraction procedure for the purpose of general phytochemical characterization, and compare this to a 95 % aqueous ethanol extraction technique. From the results it is clear that A. greatheadii contains a variety of compounds with confirmed antioxidant capacity and other putative health benefits (such as blood glucose, cholesterol and cortisol lowering properties relating to the prevention or treatment of diabetes, cardiovascular disease, cancer and hypertension. The results also indicate that separate ethyl acetate/diethyl ether and hexane extractions of the LGE, better serve for general phytochemical characterization purposes, and 95 % aqueous ethanol extraction for concentrating selective groups of health related compounds, hence justifying its use for biological in vivo efficacy studies.

  9. Wound healing activity of flower extract of Calendula officinalis.

    Science.gov (United States)

    Preethi, Korengath C; Kuttan, Ramadasan

    2009-01-01

    The effects of oral and topical application of Calendula officinalis flower extract on excision wounds made in rats were checked. The parameters assessed were the days needed for re-epithelization and percentage of wound closure. The hydroxy proline and hexosamine content in the granuloma tissue of the wound was also measured. The percentage of wound closure was 90.0% in the extract-treated group, whereas the control group showed only 51.1% on the eighth day of wounding (p officinalis extract.

  10. Mosquitocidal and water purification properties of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus leaf extracts.

    Science.gov (United States)

    Ethanolic extracts of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus were tested for toxicity to 3rd instar Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti. Median lethal concentrations (LC50) were, respectively, 0.44%, 0.51%, 0.59% and 0.68%. Cynodon dactylon...

  11. DETERMINATION OF ANTIMICROBIAL ACTIVITY OF EXTRACTS OF CALENDULA OFFICINALIS FLOWERS

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    P. V. Afanasyeva

    2016-01-01

    Full Text Available Pot marigold (Calendula officinalis L. is one of the most popular medicinal plants in the Russian Federation and abroad. The wide range of pharmacological activity of this medicinal plant is determined by carotenoids, flavonoids, saponins. These biologically active substances give total therapeutic effect of flowers of Calendula officinalis and medicines on base of pot marigold. This paper discusses the results of comparative investigations for a determination of antimicrobial activity of aqueous and aqueous- alcoholic extracts from pot marigold flowers. Detection of the minimum inhibitory concentration (MIC was carried out by using the method of double serial dilutions in broth. The following microorganisms were used as test cultures: Bacillus cereus, Candida albicans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The study showed that the widest spectrum of antibacterial activity has water extract of pot marigold flowers. As for Pseudomonas aeruginosa the most active medicine is tincture (1:10 with 70% alcohol. As for Escherichia coli the only phytopharmaceutical – water extract of marigold flowers, reveals antimicrobial activity. Against Bacillus cereus the most effective properties was indicated for tincture (1:5 with 70% ethanol and the liquid extract (1:2 with 70% alcohol. In case of Candida albicans, tincture (1:10 with 70% alcohol exhibited the highest activity.

  12. Phytochemical analysis and acaricidal activity of Aloe arborescens Mill. extracts against Rhipicephalus (Boophilus microplus

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    Aldair Calistro de Matos

    2017-10-01

    Full Text Available The indiscriminate use of chemical acaricides has allowed Rhipicephalus (Boophilus microplus to develop resistance to several active principles. However, botanical extracts have been tested as an alternative method to control those ticks. This experiment studied the chemical fingerprint and acaricidal effect of fresh and dry Aloe arborescens Mill. extracts on R. (B. microplus. The acaricidal activity of extracts was assessed using in vitro assays with engorged females, and phytochemical characterization was performed by infrared (IR spectroscopy and high-performance liquid chromatography (HPLC. The results showed that fresh and dry A. arborescens extracts prepared with the solvents pure ethanol, ethanol-dichloromethane binary mixture, and ethanol-dichloromethane-acetone ternary mixture, contained water-soluble tannins and had a strong effect on the reproductive parameters of R. (B. microplus demonstrated by a marked decreased in the number of eggs laid and in the larvae hatching rate (p < 0.05, p < 0.01, p < 0.001. In conclusion, A. arborescens Mill. has components with acaricidal activity against R. (B. microplus, and phytotherapy with extracts of this plant may be used as an alternative method of R. (B. microplus control.

  13. Encapsulation of Aloe Vera extract into natural Tragacanth Gum as a novel green wound healing product.

    Science.gov (United States)

    Ghayempour, Soraya; Montazer, Majid; Mahmoudi Rad, Mahnaz

    2016-12-01

    Application of natural materials in wound healing is an interest topic due to effective treatment with no side effects. In this paper, Aloe Vera extract was encapsulated into Tragacanth Gum through a sonochemical microemulsion process to prepare a wound healing product. FESEM/EDX and FT-IR proved the successfully formation of the nanocapsules with spherical shape by cross-linking aluminum ions with Tragacanth Gum. The therapeutic characteristics of the prepared wound healing product were investigated using antimicrobial, cytotoxicity and wound healing assays. Relative high antimicrobial activities with the microbial reduction of 84, 91 and 80% against E. coli, S. aureus and C. albicans, a cell viability of 98% against human fibroblast cells and a good wound healing activity with considerable migration rate of fibroblast cells are the important advantages of the new formed wound healing product. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. A novel topical association with zinc oxide, chamomile and aloe vera extracts - stability and safety studies

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    Catarina Reis

    2015-12-01

    Full Text Available Currently, natural products show an enormous potential for pharmaceutical and cosmetic industries. The goals of this study were to formulate and to characterise a novel combination of natural products. Formulations were 1 an oil-in-water emulsion, 2 a water-in-oil emulsion and 3 a cleansing solution. Zinc oxide was chosen as an active ingredient due to its healing properties, and chamomile and aloe vera extracts were chosen due to their antiseptic, anti-inflammatory and tissue regenerating properties. Organoleptic characteristics, pH, viscosity and in vitro efficacy for the most common bacteria and yeast of human skin were evaluated. Preliminary and accelerated stability studies and safety tests were also performed. All optimized products were stable, smooth in texture, effective against bacteria and yeast, and safe, justifying further studies. Results suggest that these novel products might be a promising source of natural compounds with soothing and regenerative properties for skin care.

  15. Magnetic nanoporous carbon as an adsorbent for the extraction of phthalate esters in environmental water and aloe juice samples.

    Science.gov (United States)

    Liu, Li; Hao, Yunhui; Ren, Yiqian; Wang, Chun; Wu, Qiuhua; Wang, Zhi

    2015-05-01

    In this work, magnetic nanoporous carbon with high surface area and ordered structure was synthesized using cheap commercial silica gel as template and sucrose as the carbon source. The prepared magnetic nanoporous carbon was firstly used as an adsorbent for the extraction of phthalate esters, including diethyl phthalate, diallyl phthalate, and di-n-propyl-phthalate, from lake water and aloe juice samples. Several parameters that could affect the extraction efficiency were optimized. Under the optimum conditions, the limit of detection of the method (S/N = 3) was 0.10 ng/mL for water sample and 0.20 ng/mL for aloe juice sample. The linearity was observed over the concentration range of 0.50-150.0 and 1.0-200.0 ng/mL for water and aloe juice samples, respectively. The results showed that the magnetic nanoporous carbon has a high adsorptive capability toward the target phthalate esters in water and aloe juice samples. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Psidium guajava and Piper betle Leaf Extracts Prolong Vase Life of Cut Carnation (Dianthus caryophyllus) Flowers

    Science.gov (United States)

    Rahman, M. M.; Ahmad, S. H.; Lgu, K. S.

    2012-01-01

    The effect of leaf extracts of Psidium guajava and Piper betle on prolonging vase life of cut carnation flowers was studied. “Carola” and “Pallas Orange” carnation flowers, at bud stage, were pulsed 24 hours with a floral preservative. Then, flowers were placed in a vase solution containing sprite and a “germicide” (leaf extracts of P. guajava and P. betle, 8-HQC, or a copper coin). Flowers treated with 8-HQC, copper coin, and leaf extracts had longer vase life, larger flower diameter, and higher rate of water uptake compared to control (tap water). The leaf extracts of P. guajava and P. betle showed highest antibacterial and antifungal activities compared to the other treatments. Both showed similar effects on flower quality as the synthetic germicide, 8-HQC. Therefore, these extracts are likely natural germicides to prolong vase life of cut flowers. PMID:22619568

  17. Psidium guajava and Piper betle leaf extracts prolong vase life of cut carnation (Dianthus caryophyllus) flowers.

    Science.gov (United States)

    Rahman, M M; Ahmad, S H; Lgu, K S

    2012-01-01

    The effect of leaf extracts of Psidium guajava and Piper betle on prolonging vase life of cut carnation flowers was studied. "Carola" and "Pallas Orange" carnation flowers, at bud stage, were pulsed 24 hours with a floral preservative. Then, flowers were placed in a vase solution containing sprite and a "germicide" (leaf extracts of P. guajava and P. betle, 8-HQC, or a copper coin). Flowers treated with 8-HQC, copper coin, and leaf extracts had longer vase life, larger flower diameter, and higher rate of water uptake compared to control (tap water). The leaf extracts of P. guajava and P. betle showed highest antibacterial and antifungal activities compared to the other treatments. Both showed similar effects on flower quality as the synthetic germicide, 8-HQC. Therefore, these extracts are likely natural germicides to prolong vase life of cut flowers.

  18. The Anti-Nociceptive Effect of Aloe. Vera Aqueous Extract in Fructose-Fed Male Rats

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    Mohammad Reza Shahraki

    2010-05-01

    Full Text Available A B S T R A C T Introduction: Aloe Vera extract is used as an anti-inflammatory and anti-bradikinin agent in laboratory animals. The aim of this survey was to evaluate the ant-nociceptive effect of A. Vera aqueous extract in fructose-fed male rats. Methods: Forty-five Wistar-Albino male rats were equally and randomly divided into five groups including sham operated and four test groups. Sham operated group consumed tap water and the test groups consumed fructoseenriched water. Test groups 2, 3 and 4 additionally received, 0, 100, 150 and 200 mg/kg of A. Vera extract, respectively, whereas the other test group received distilled water daily. Tail flick reaction time, serum glucose and oral glucose tolerance test (OGTT were measured. The results were analyzed by SPSS software using ANOVA and Tukey tests. Results were expressed as mean ± SD. Statistical differences were considered significant at p<0.05. Results: The results showed that tail flick reaction time significantly increased in test group 3 which received 200 mg/kg A. Vera extract comparing with that of sham operated group. However, OGTT and serum glucose value were significantly increased in all fructose-fed male rats comparing with those of sham operated group. Discussion: These results indicated that A. Vera aqueous extract can affect tail flick reaction time in fructose-fed male rats. Further studies are required to show the exact mechanism of anti-nociceptive effect of A. Vera extract.

  19. Beneficial effects of aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes.

    Science.gov (United States)

    Rajasekaran, Subbiah; Ravi, Kasiappan; Sivagnanam, Karuran; Subramanian, Sorimuthu

    2006-03-01

    The effect of diabetes mellitus on lipid metabolism is well established. The association of hyperglycaemia with an alteration of lipid parameters presents a major risk for cardiovascular complications in diabetes. Many secondary plant metabolites have been reported to possess lipid-lowering properties. The present study was designed to examine the potential anti-hyperlipidaemic efficacy of the ethanolic extract from Aloe vera leaf gel in streptozotocin (STZ)-induced diabetic rats. 2. Oral administration of Aloe vera gel extract at a dose of 300 mg/kg bodyweight per day to STZ-induced diabetic rats for a period of 21 days resulted in a significant reduction in fasting blood glucose, hepatic transaminases (aspartate aminotransferase and alanine aminotransferase), plasma and tissue (liver and kidney) cholesterol, triglycerides, free fatty acids and phospholipids and a significant improvement in plasma insulin. 3. In addition, the decreased plasma levels of high-density lipoprotein-cholesterol and increased plasma levels of low-density lipoprotein-and very low-density lipoprotein-cholesterol in diabetic rats were restored to near normal levels following treatment with the extract. 4. The fatty acid composition of the liver and kidney was analysed by gas chromatography. The altered fatty acid composition in the liver and kidney of diabetic rats was restored following treatment with the extract. 5. Thus, the results of the present study provide a scientific rationale for the use of Aloe vera as an antidiabetic agent.

  20. Aphrodisiac potentials of the ethanol extract of Aloe barbadensis Mill. root in male Wistar rats.

    Science.gov (United States)

    Erhabor, Joseph O; Idu, MacDonald

    2017-07-11

    Aloe barbadensis (AB) is a short stemmed succulent medicinal herb that is being used by locals in Nigeria to enhance libido. Therefore this study evaluates the aphrodisiac potential and acute toxicological effect of A. barbadensis (AB) root in male Wistar rats. Aphrodisiac potential was determined following the oral administration of graded doses (100, 200 and 400 mg/kg) of ethanol extract of A. barbadensis root. Sildenafil citrate (Viagra) and distilled water served as positive and negative controls respectively. Sexual behavioural parameters (mounting and intromission frequencies, mounting, intromission and ejaculatory latencies) were observed. Serum testosterone and cholesterol concentrations were also progressively monitored on days 1, 7 and 14. The acute toxicological evaluation of the plant were based on any onset behavioural changes and mortality respectively. The findings from the sexual behavioural study indicated that the ethanol extract of A. barbadensis significantly increased mounting frequency and intromission frequency but significantly decreased mount and intromission latencies in a dose dependent manner particularly on day 1 and 14. The ethanol extract also prolonged ejaculatory latency. The testosterone and cholesterol concentrations were also increased as the dose increased particularly on day 1 and 7. The lowest dose of 100 mg/kg showed the best aphrodisiac effect. The toxicity studies showed that there were no acute behavioural changes with zero mortality. The increased blood testosterone and cholesterol concentrations by the ethanol extract of A. barbadensis can probably be said to be the possible mechanisms of action for its aphrodisiac property. The plant may also be used to treat hypotestosteronemia following its ability to increase testosterone. These findings therefore give backing to the acclaimed local use of A. barbadensis root as an aphrodisiac in males.

  1. Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity.

    Science.gov (United States)

    Logaranjan, Kaliyaperumal; Raiza, Anasdass Jaculin; Gopinath, Subash C B; Chen, Yeng; Pandian, Kannaiyan

    2016-12-01

    Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses and further supported by surface-enhanced Raman spectroscopy/Raman scattering (SERS) study. UV-visible spectrum has shown a sharp peak at 420 nm and further evidenced by FTIR peak profile (at 1587.6, 1386.4, and 1076 cm -1 with corresponding compounds). The main band position with SERS was noticed at 1594 cm -1 (C-C stretching vibration). When samples were heated under microwave radiation, AgNP with octahedron shapes with 5-50 nm were found and this method can be one of the easier ways to synthesis anisotropic AgNP, in which the plant extract plays a vital role to regulate the size and shape of the nanoparticles. Enhanced antibacterial effects (two- to fourfold) were observed in the case of Aloe vera plant protected AgNP than the routinely synthesized antibiotic drugs. Shape and size-controlled synthesis of silver nanoparticles using Aloe vera plant extract.

  2. Haemato-immunological indices in rainbow trout (Oncorhynchus mykiss fry fed with Aloe vera extract supplemented feed

    Directory of Open Access Journals (Sweden)

    Masoud Haghighi

    2014-05-01

    Full Text Available Objective: To evaluate the effects of Aloe vera extract on the immunity responses and haematological parameters in rainbow trout (Oncorhynchus mykiss fry to develop alternative drug to chemotherapeutics in aquaculture. Methods: Six hundred rainbow trout (Oncorhynchus mykiss fry were randomly allocated into two treatment groups including 1 placebo-treated group (control, 2 Aloe vera extract-treated group, each of three replicates. The fishes were hand-fed once a day with diet medicated AE or placebo at the rate of 1% in feed in the first feeding for 10 weeks. At the end of the identical every two weeks 24 h after feeding, some of haematological and immunological parameters were analyzed. Results: The results showed that serum total protein, albumin and globulin, respiratory burst activity, phagocytic activity and serum lysozyme activity vary among the two treatment groups which were found to be higher in Aloe vera extract-treated group (P0.05. Conclusions: It was concluded that supplementation of AE at a rate of 1% in feed registered higher immunological responses in compared to placebo group. Therefore, supplementation of AE in fish diets enhances non-specific immune system in fish. It may use in fish diets particularly at time of outbreaks.

  3. Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium.

    Science.gov (United States)

    Saniasiaya, Jeyasakthy; Salim, Rosdan; Mohamad, Irfan; Harun, Azian

    2017-01-01

    Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian Aloe vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans. This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC). For A. niger, a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts of Aloe vera leaf for C. albicans . The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone of inhibition was obtained for C. albicans the MIC was not determined. The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the aqueous extract for A. niger ( p Aloe vera has a significant antifungal effect towards A. niger.

  4. Plasma-assisted deposition of microcapsule containing Aloe vera extract for cosmeto-textiles

    Science.gov (United States)

    Nascimento do Carmo, S.; Zille, A.; Souto, A. P.

    2017-10-01

    Dielectric Barrier Discharge (DBD) atmospheric-pressure plasma was employed to enhance the deposition of commercial microcapsules (MCs) containing Aloe vera extract onto a cotton/polyester (50:50) fabric. DBD conditions were optimized in term of energy dosage and contact angle. The MCs were applied by padding and printing methods and the coatings were characterized in terms of SEM and FTIR. MCs display a spherical shape with size between 2 and 8 μm with an average wall thickness of 0.5 μm. The MCs applied by printing and pretreated with a plasma dosage of 1.6 kW m2 min-1 showed the best results with an increased adhesion of 200% and significant penetration of MCs into the fibres network. Plasma printed fabric retained 230% more MCs than untreated fabric after 10 washing cycles. However, the coating resistance between unwashed and washed samples was only improved by 5%. Considering the fact that no binder or crosslinking agents were used, the DBD plasma-assisted deposition of MCs revealed to be a promising environmental safe and low cost coating technology.

  5. Radiomodification by Aloe vera leaf extract on skin of Swiss albino mice exposed to gamma radiation

    International Nuclear Information System (INIS)

    Gehlot, Prashasnika; Soyal, Dhanraj; Goyal, P.K.

    2007-01-01

    The development of effective radioprotectors and radiorecovery drugs is of great importance in view of their potential application during both planned (e.g. radiotherapy) and unplanned (e.g. in nuclear industry, natural background radiation emanating from the earth or other sources) radiation exposure. Over the past 50 years, research in the development of radioprotectors has focused on screening a plethora of chemical and biological compounds. Several synthetic chemical compounds have been tested for protection against radiation. But they have limited use due to inherent toxicity. Herbal medicine is still the mainstay of about 75-80 percent of the world population mainly in the developing nations for primary health care because of better cultural acceptability, better compatibility with the human body and lesser side effects. Thus, natural products offer an alternative to their synthetic counterparts due to low toxicity with no side effects. The present investigation has been an attempt to asses the radioprotective efficacy of Aloe vera leaf extract on biochemical alterations in skin of Swiss albino mice

  6. The effect of Aloe vera extract on humoral and cellular immune ...

    African Journals Online (AJOL)

    . G Vahedi, M Taghavi, AK Maleki, R Habibian. Abstract. Some plant polysaccharides are well known to possess immunostimulatory effects. Aloe vera possesses confirmed curative or healing actions. The aim of this study was to evaluate the ...

  7. Wound healing potential of flowers extracts of Woodfordia fruticosa Kurz.

    Science.gov (United States)

    Verma, Neeraj; Amresh, G; Sahu, P K; Mishra, Neelam; Rao, Ch V; Singh, Anil Pratap

    2013-08-01

    Wound healing or repair is the body's natural process of regenerating dermal and epidermal tissue. Woodfordia fruticosa Kurz (Family: Lythraceae) is used traditionally in wound healing by the tribals of Chhattisgarh district. However, there is a paucity of scientific data in support. In this study, we evaluated antimicrobial activity of petroleum ether, chloroform, ethanolic and aqueous extracts against a diverse range of gram +ve and gram -ve bacteria along with pathogenic fungi. The wound healing activity of ethanolic extract was also evaluated at dose levels of 250 and 500 mg/kg body wt in rats by excision, incision and dead space wound healing models along with histopathology of wound area of skin. The ethanolic extract showed potent wound healing activity, as evident from the increase in the wound contraction and breaking strength in dose-dependent manner. Treatment with ethanolic extract (250 and 500 mg/kg body wt) showed significant dose-dependently decrease in epithelization period and scar area. Hydroxyproline, hexuronic acid and hexosamine contents, the important constituents of extracellular matrix of healing were also correlated with the observed healing pattern. During early wound healing phase, pro-inflammatory cytokines TNF-alpha, IL-6 and anti-inflammatory cytokine IL-10 levels were found to be upregulated by the ethanolic extract treatment. The ethanolic extract exhibited a strong and broad spectrum antimicrobial activity, as compared to other extracts. It showed very low Minimum inhibitory concentration (MIC) values and inhibited the growth of E. coli, Staphylococcus aureus and Candida albicans in concentration of 2.5 microg/disc. Thus, the results of the present study demonstrated the strong wound healing potential and antimicrobial activities of W. fruticosa, flowers, supporting the folklore use of the plant by the tribal people of Chhattisgarh district.

  8. Evaluation of wound healing treated with latex derived from rubber trees and Aloe Vera extract in rats.

    Science.gov (United States)

    Brandão, Marcelo Luiz; Reis, Paulo Roberto Melo; Araújo, Lilhian Alves de; Araújo, Ana Carolina Vicente; Santos, Maisa Helena de Abreu Silva; Miguel, Marina Pacheco

    2016-09-01

    To compare the use of latex derivative and Aloe vera extract to wound healing. Twenty one rats were randomly divided into three groups and each one had a wound made by incision. The treatment consisted in: derivative of latex (GL), Aloe vera extract (GA) and saline solution (GC). The wound area was measured on the 7th, 14th and 21st days and macroscopic and microscopic evaluation were done. The comparison between the measurements of the wounds presented statistical difference in GC and GA from the 7th day of evaluation and GL from the 14th day. The extent of the wound was significantly smaller by the 7th day in GL. Histologically, in GL, the neovascularization was significant on the 7th, 14th and 21st days. On the 21st day the scar was large and little mature. In GA and GC, the findings were similar on the 7th, 14th and 21st days with a slight better organization of skin and collagen on the 21st in GA. Statistical analysis did not allow for the definition of the best topical agent. The latex had the highest angiogenesis, but a possible foreign body granuloma. Aloe vera has revealed a healing process adequated temporally in histology.

  9. Extraction and characterization of proteins from banana (Musa Sapientum L) flower and evaluation of antimicrobial activities.

    Science.gov (United States)

    Sitthiya, Kewalee; Devkota, Lavaraj; Sadiq, Muhammad Bilal; Anal, Anil Kumar

    2018-02-01

    Ultrasonic assisted alkaline extraction of protein from banana flower was optimized using response surface methodology. The extracted proteins were characterized by Fourier transform infrared spectroscopy and molecular weight distribution was determined by gel electrophoresis. The maximum protein yield of 252.25 mg/g was obtained under optimized extraction conditions: temperature 50 °C, 30 min extraction time and 1 M NaOH concentration. The alkaline extraction produced a significantly high protein yield compared to enzymatic extraction of banana flower. Chemical finger printing of proteins showed the presence of tyrosine, tryptophan and amide bonds in extracted protein. Alkaline and pepsin assisted extracted banana flower proteins showed characteristic bands at 40 and 10 kDA, respectively. The extracted proteins showed antibacterial effects against both gram positive and gram negative bacteria. The high protein content and antimicrobial activity indicate the potential applications of banana flower in the food and feed industry.

  10. Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract

    Directory of Open Access Journals (Sweden)

    Huang Huey-Chun

    2012-06-01

    Full Text Available Abstract Background Magnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. In the study, the inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated. Results Our results revealed that M. grandiflora L. flower extract inhibit mushroom tyrosinase activity (IC50 =11.1%; v/v, the flower extract also effectively suppressed intracellular tyrosinase activity (IC50 = 13.6%; v/v and decreased the amount of melanin (IC50 = 25.6%; v/v in a dose-dependent manner in B16F10 cells. Protein expression level of tyrosinase and tyrosinase-related protein 1 (TRP-1 were also decreased by the flower extract. Additionally, antioxidant capacities such as ABTS+ free radical scavenging activity, reducing capacity and total phenolic content of the flower extract were increased in a dose-dependent pattern. Conclusions Our results concluded that M. grandiflora L. flower extract decreased the expression of tyrosinase and TRP-1, and then inhibited melanogenesis in B16F10 cells. The flower extract also show antioxidant capacities and depleted cellular reactive oxygen species (ROS. Hence, M. grandiflora L. flower extract could be applied as a type of dermatological whitening agent in skin care products.

  11. In vivo diabetic wound healing effect and HPLC–DAD–ESI–MS/MS profiling of the methanol extracts of eight Aloe species

    Directory of Open Access Journals (Sweden)

    Abeer M. El Sayed

    Full Text Available Abstract Genus Aloe, Xanthorrhoeaceae, is well distributed all over Egypt, and many species have been used as medicinal plants; mainly reported to prevent cardiovascular diseases, cancer and diabetes. This study attempts to analyze the secondary metabolites in the methanol extract of the leaves of eight Aloe species; A. vera (L. Burm. f., A. arborescens Mill., A. eru A. Berger, A. grandidentata Salm-Dyck, A. perfoliata L., A. brevifolia Mill., A. saponaria Haw. and A. ferox Mill. growing in Egypt. For this aim HPLC–DAD–MS/MS in negative ion mode was used. Although belonging to the same genus, the composition of each species presented different particularities. Seventy one compounds were identified in the investigated Aloe species, of which cis-p-coumaric acid derivaties, 3,4-O-(E caffeoylferuloylquinic acid and caffeoyl quinic acid hexoside were the most common phenolic acids identified. Aloeresin E and isoaloeresin D, 2'-O-feruloylaloesin were the common anthraquinones identified. Lucenin II, vicenin II, and orientin were the common identified flavonoids in the investigated Aloe species. 6'-Malonylnataloin, aloe-emodin-8-O-glucoside, flavone-6,8-di-C-glucosides could be considered as chemotaxonomic markers for the investigated Aloe species. The eight Aloe species had significant anti-inflammatory activity, in addition to the significant acceleration of diabetic wound healing in rats following topical application of the methanol extracts of their leaves. This is the first simultaneous characterization and qualitative determination of multiple phenolic compounds in Aloe species from locally grown cultivars in Egypt using HPLC–DAD–MS/MS, which can be applied to standardize the quality of different Aloe species and the future design of nutraceuticals and cosmetic preparations.

  12. In vivo diabetic wound healing effect and HPLC–DAD–ESI–MS/MS profiling of the methanol extracts of eight Aloe species

    OpenAIRE

    El Sayed, Abeer M.; Ezzat, Shahira M.; El Naggar, Moataz M.; El Hawary, Seham S.

    2016-01-01

    Abstract Genus Aloe, Xanthorrhoeaceae, is well distributed all over Egypt, and many species have been used as medicinal plants; mainly reported to prevent cardiovascular diseases, cancer and diabetes. This study attempts to analyze the secondary metabolites in the methanol extract of the leaves of eight Aloe species; A. vera (L.) Burm. f., A. arborescens Mill., A. eru A. Berger, A. grandidentata Salm-Dyck, A. perfoliata L., A. brevifolia Mill., A. saponaria Haw. and A. ferox Mill. growing in ...

  13. Antiviral activity of Aloe hijazensis against some haemagglutinating viruses infection and its phytoconstituents.

    Science.gov (United States)

    Abd-Alla, Howaida I; Abu-Gabal, Nagat S; Hassan, Amal Z; El-Safty, Mounir M; Shalaby, Nagwa M M

    2012-08-01

    Evaluation of the antiviral activities of flowers, flower-peduncles, leaves, and roots of Aloe hijazensis against haemagglutinating viruses of avian paramyxovirus type-1 (APMV-1), avian influenza virus type A (AI-H5N1), Newcastle disease virus (NDV), and egg-drop syndrome virus (EDSV) in specific pathogen free (SPF) chicken embryos were carried out. Extract of the flowers and leaves showed relatively higher activity than the extracts of other plant parts. Thirteen compounds were isolated from both the flowers and flower-peduncles of A. hijazensis. The isolated compounds were classified into: five anthraquinones; ziganein, ziganein-5-methyl ether, aloesaponarin I, chrysophanol, aloe-emodin, one dihydroisocoumarin; feralolide, four flavonoids; homoplantaginin, isoorientin, luteolin 7-glucuronopyranoside, isovitexin, one phenolic acid; p-coumaric acid, the anthrone; barbaloin together with aloenin. Eleven compounds were attributed to the flowers and seven to the flower-peduncles. Homoplantaginin and luteolin 7-glucuronopyranoside are reported here for the first time from Aloe spp. To the best of our knowledge, this is the first report on the chemical composition and biological activity of those plant parts.

  14. Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress

    Directory of Open Access Journals (Sweden)

    Fábio Sabbadin Zanuzzo

    2012-10-01

    Full Text Available This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus, to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS only at 0.1, 0.2, 0.5 and 1 mg/L. Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested.

  15. Antioxidant activities of aqueous extracts from 12 Chinese edible flowers in vitro and in vivo

    OpenAIRE

    Wang, Feng; Miao, Miao; Xia, Hui; Yang, Li-Gang; Wang, Shao-Kang; Sun, Gui-Ju

    2016-01-01

    ABSTRACT The antioxidant function of edible flowers have attracted increasing interest. However, information is lacking on the impact of edible flowers on oxidative injury including hypoxia-re-oxygenation and hyperlipidemia. The antioxidant activities of aqueous extracts from 12 Chinese edible flowers were assessed in four different antioxidant models, including total antioxidant capacity (TAC), oxygen radical absorbance capacity (ORAC), scavenging hydroxyl radical capacity (SHRC) and scaveng...

  16. Preliminary studies on the efficacy of aloe vera ( Aloe barbadensis ...

    African Journals Online (AJOL)

    Extracts of Aloe vera were administered to experimentally infected mice with the Nigeria strain of Trypanosoma brucei brucei. The thirty-two (32) mice involved in the study were split into eight (8) groups of 4 mice each and treated intraperitoneally with the aqueous and ethanolic Aloe vera extracts of 40, 80, 20 mg/Kg body ...

  17. Assessment of the Safety of Aqueous Extract of Aloe vera on ...

    African Journals Online (AJOL)

    Aloe vera is used both traditionally and packaged commercially in many regions of the world for several medicinal and or cosmetic purposes. It is claimed to have rejuvenating, moisturizing, healing or soothing properties on the skin and gastrointestinal tract. This study focused on assessment of the safety of A. vera on blood ...

  18. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

    Directory of Open Access Journals (Sweden)

    Yun Sun Lee

    Full Text Available Aloe vera (Asphodeloideae is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  19. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

    Science.gov (United States)

    Lee, Yun Sun; Ju, Hyun Kyoung; Kim, Yeon Jeong; Lim, Tae-Gyu; Uddin, Md Romij; Kim, Yeon Bok; Baek, Jin Hong; Kwon, Sung Won; Lee, Ki Won; Seo, Hak Soo; Park, Sang Un; Yang, Tae-Jin

    2013-01-01

    Aloe vera (Asphodeloideae) is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  20. Dietary aloe vera gel powder and extract inhibit azoxymethane- induced colorectal aberrant crypt foci in mice fed a high- fat diet.

    Science.gov (United States)

    Chihara, Takeshi; Shimpo, Kan; Kaneko, Takaaki; Beppu, Hidehiko; Higashiguchi, Takashi; Sonoda, Shigeru; Tanaka, Miyuki; Yamada, Muneo; Abe, Fumiaki

    2015-01-01

    Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

  1. An evaluation of the impact of aloe vera and licorice extracts on the course of experimental pigeon paramyxovirus type 1 infection in pigeons.

    Science.gov (United States)

    Dziewulska, D; Stenzel, T; Smialek, M; Tykalowski, B; Koncicki, A

    2018-02-01

    The progressive decrease in the efficiency of synthetic drugs has prompted research into phytogenic feed additives with potentially immunomodulatory and anti-infective properties. Complex diseases with a mixed etiology, including viral, pose a growing problem in domestic pigeons. The aim of this study was to determine the effectiveness of various doses of aloe vera and licorice extracts on the course of experimental PPMV-1 infection in pigeons. The experiment was performed on pigeons divided into 5 groups, including one control group and 4 experimental groups, which were orally administered aloe vera or licorice extracts at 300 or 500 mg/kg BW for 7 d after experimental inoculation with PPMV-1. On d 4, 7, and 14 after inoculation, cloacal swabs and samples of organs were collected from 4 birds in each group. The samples were analyzed to determine the copy number of PPMV-1 RNA by TaqMan qPCR. The results indicate that licorice and aloe vera extracts inhibited PPMV-1 replication by decreasing viral RNA copy numbers in the examined organs. The most inhibitory effect was observed in pigeons receiving aloe vera extract at 300 mg/kg BW, for which PPMV-1 RNA copy numbers were approximately 7-fold lower (brain), 9-fold lower (kidneys), and 14-fold lower (liver) than in the control group. The results of this study point to the potentially antiviral effects of aloe vera and licorice extracts in pigeons infected with PPMV-1. To the best of our knowledge, this is the first study to investigate the antiviral properties of aloe vera and licorice extracts in domestic pigeons. © The Author 2017. Published by Oxford University Press on behalf of Poultry Science Association.

  2. Effect of Gelatin-Based Edible Coatings Incorporated with Aloe vera and Black and Green Tea Extracts on the Shelf Life of Fresh-Cut Oranges

    Directory of Open Access Journals (Sweden)

    Mohsen Radi

    2017-01-01

    Full Text Available The aim of this study was to evaluate the effect of gelatin coating incorporated with Aloe vera gel (50,100% and green and black tea extracts (5,10% on physicochemical, microbial, and sensorial properties of fresh-cut oranges at 4°C for 17 days. Significant differences in terms of quality parameters were observed between the control and coated fresh-cut oranges. The highest variation of quality parameters was observed in control, while the least variations were observed in coated slices with 100% Aloe vera and 10% green tea extract. The weight loss was increased with time, but the coating treatment especially with 100% Aloe vera had significant effect on the prevention of weight loss. Also, Aloe vera coated samples obtained the highest score in sensory evaluation. Coating with gelatin incorporated with Aloe vera and green tea extracts successfully retarded the microbial growth and therefore extended the shelf life of fresh-cut oranges during cold storage.

  3. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

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    Patcharaporn Tippayawat

    2016-10-01

    Full Text Available Background There is worldwide interest in silver nanoparticles (AgNPs synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV with antibacterial activity. Methods AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively

  4. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity.

    Science.gov (United States)

    Tippayawat, Patcharaporn; Phromviyo, Nutthakritta; Boueroy, Parichart; Chompoosor, Apiwat

    2016-01-01

    There is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity. AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa . The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 o C for 6 h and 200 o C for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in pharmaceutical, biotechnological

  5. Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya

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    Utari Kresnoadi

    2014-03-01

    Full Text Available Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb. As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan

  6. Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity

    Science.gov (United States)

    Logaranjan, Kaliyaperumal; Raiza, Anasdass Jaculin; Gopinath, Subash C. B.; Chen, Yeng; Pandian, Kannaiyan

    2016-11-01

    Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses and further supported by surface-enhanced Raman spectroscopy/Raman scattering (SERS) study. UV-visible spectrum has shown a sharp peak at 420 nm and further evidenced by FTIR peak profile (at 1587.6, 1386.4, and 1076 cm-1 with corresponding compounds). The main band position with SERS was noticed at 1594 cm-1 (C-C stretching vibration). When samples were heated under microwave radiation, AgNP with octahedron shapes with 5-50 nm were found and this method can be one of the easier ways to synthesis anisotropic AgNP, in which the plant extract plays a vital role to regulate the size and shape of the nanoparticles. Enhanced antibacterial effects (two- to fourfold) were observed in the case of Aloe vera plant protected AgNP than the routinely synthesized antibiotic drugs.

  7. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats

    OpenAIRE

    Nayak, BS; Pinto Pereira, Lexley M

    2006-01-01

    Abstract Background Catharanthus roseus L (C. roseus) has been used to treat a wide assortment of diseases including diabetes. The objective of our study was to evaluate the antimicrobial and wound healing activity of the flower extract of Catharanthus in rats. Methods Wound healing activity was determined in rats, after administration (100 mg kg-1 day-1) of the ethanol extract of C. roseus flower, using excision, incision and dead space wounds models. The animals were divided into two groups...

  8. Effects of Aloe barbadensis Mill. extract (AVH200®) on human blood T cell activity in vitro.

    Science.gov (United States)

    Ahluwalia, Bani; Magnusson, Maria K; Isaksson, Stefan; Larsson, Fredrik; Öhman, Lena

    2016-02-17

    Aloe barbadensis Mill. (Aloe vera) is a widely used medicinal plant well reputed for its diverse therapeutic applications. It has been used for thousands of years in folk medicine to treat various conditions and the Aloe vera gel has been reported to possess anti-inflammatory as well as immunostimulatory and immunomodulatory properties. However, the mode of action is still unclear. The aim of this study was determine the effects of two well-defined A. barbadensis Mill. extracts AVH200® and AVE200 on human blood T cells in vitro. Peripheral blood mononuclear cells (PBMC) from healthy donors were stimulated polyclonally in the presence or absence of AVH200® and AVE200. The T cell phenotype was investigated by flow cytometry, cell proliferation was determined by CFSE dye and thymidine assay, respectively and cytokine secretion was determined by MSD® Multi-Spot Assay system and ELISA. The presence of AVH200® resulted in a reduced expression of CD25 among CD3(+) T cells and suppression of T cell proliferation in a dose dependent manner. Furthermore, AVH200® reduced the expression of CD28 on CD3(+) T cells. AVH200® also reduced the secretion of IL-2, IFN-γ and IL-17A in PBMC cultures. The AVH200® dose dependent reduction in T cell activation and proliferation recorded in the cell cultures was not due to apoptosis or cell death. Additionally, AVH200® was found to be more effective as compared to AVE200 in reducing T cell activation and proliferation. AVH200® has the potential to reduce the activation, proliferation and cytokine secretion of healthy human blood T cells. Our study suggests that AVH200® has a suppressive effect on human blood T cells in vitro. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  9. Effectiveness of Aloe vera leaf extract against low level exposure to gamma radiation induced injury in intestinal mucosa of Swiss mice

    International Nuclear Information System (INIS)

    Gehlot, Prashasnika; Saini, M.R.

    2004-01-01

    Full Text: Human beings can not deny the presence of all sorts of incoming radiations, which are detrimental to life. The small intestine represents one of the major dose limiting normal tissues in radiotherapy because of its high radio sensitivity. The purpose of this study was to determine the effects of Aloe vera, a potential radioprotector. Radioprotective efficacy of aloe vera leaf extract in intestinal mucosa in mice (1 g/kg body weight/day) was studied from 6h to day 20 after gamma irradiation (0.5 Gy(. Villus height, goblet cells/villus section, total cells are good parameters for the assessment of radiation damage. The mice selected from inbreed colony were divided into two groups. The first group was given Aloe vera extract orally for 15 consecutive days and served as experimental group. On 15th day, after 30 min of above treatment animals of both the groups were exposed to 0.5 Gy gamma irradiation and autopsied on 6, 12, 24 h and 5, 10, 20 days. Aloe vera pretreatment resulted in a significant increase (p<0.001) in villus height, total cells whereas globlet cells showed a significant decrease (p<0.001) from respective irradiated controls at each autospy day. The results suggest that Aloe vera pretreatment provides protection against radiation-induced alterations in intestinal mucosa of Swiss mice

  10. Utilization of plant bioactives as feed additives for poultry: The effect of Aloe vera gel and its extract on performance of broilers

    Directory of Open Access Journals (Sweden)

    A.P Sinurat

    2003-10-01

    Full Text Available Feed additives are commonly added in poultry feed as a growth promotant or to improve feed efficiency. The most common feed additive used is antibiotic at sub-therapheutic doses, although there is a controversy on its impact on human health. Previous results showed that Aloe vera gel could improve feed efficiency in broilers and an in vitro study showed that the extract have an antibacterial effect. Therefore, a further experiment was designed to study the response of broilers to Aloe vera gel or its extract as feed additives. Aloe vera was prepared in dry gel or chloroform-extract and included in the diet at levels of 0.25; 0.50 and 1.00 g/kg (equal to dry gel. Standard diets with or without antibiotic were also formulated as control and a commercial diet was included for comparison. The diets were fed to broilers from day old to 5 weeks. Each treatment has 9 replicates and 6chicks/replicate. Parameters observed were: feed consumption, weight gain and feed convertion ratios. Carcass yield, abdominal fat levels, relative weight of liver, gizzard, tractus digestivus and length of tractus digestivus were also measured at the end of feeding trial. The results showed that Aloe gel and its extract did not influence body weight gain and feed consumption of broilers significantly (P>0.05, but improved feed convertion slightly (3.50%. The response in this trial was similar as thosecommercial diet and diet added with antibiotic. There was no significant (P>0.05 effect of Aloe vera bioactives on carcass yield, abdominal fat level and relative weight of liver. However, Aloe vera gel and its extract tend to increase gizzard weight, gastro intestinal weight and length. The Aloe vera gel and its extract also reduced the total count of aerobic bacteria in the digesta of tractus digestivus. It is concluded that the Aloe vera gel improve feed efficiency in broilers by increasing the size of tractus digestivus and reducing the total count of aerobic bacteria in

  11. Aloe Vera for Tissue Engineering Applications

    Directory of Open Access Journals (Sweden)

    Shekh Rahman

    2017-02-01

    Full Text Available Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  12. Aloe Vera for Tissue Engineering Applications.

    Science.gov (United States)

    Rahman, Shekh; Carter, Princeton; Bhattarai, Narayan

    2017-02-14

    Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  13. Aloe Vera for Tissue Engineering Applications

    Science.gov (United States)

    Rahman, Shekh; Carter, Princeton; Bhattarai, Narayan

    2017-01-01

    Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers. PMID:28216559

  14. Aloe arborescens Extract Protects IMR-32 Cells against Alzheimer Amyloid Beta Peptide via Inhibition of Radical Peroxide Production.

    Science.gov (United States)

    Clementi, Maria Elisabetta; Tringali, Giuseppe; Triggiani, Doriana; Giardina, Bruno

    2015-11-01

    Aloe arborescens is commonly used as a pharmaceutical ingredient for its effect in burn treatment and ability to increase skin wound healing properties. Besides, it is well known to have beneficial phytotherapeutic, anticancer, and radio-protective properties. In this study, we first provided evidence that A. arborescens extract protects IMR32, a neuroblastoma human cellular line, from toxicity induced by beta amyloid, the peptide responsible for Alzheimer's disease. In particular, pretreatment with A. arborescens maintains an elevated cell viability and exerts a protective effect on mitochondrial functionality, as evidenced by oxygen consumption experiments. The protective mechanism exerted by A. arborescens seems be related to lowering of oxidative potential of the cells, as demonstrated by the ROS measurement compared with the results obtained in the presence of amyloid beta (1-42) peptide alone. Based on these preliminary observations we suggest that use ofA. arborescens extract could be developed as agents for the management of AD.

  15. In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts

    Directory of Open Access Journals (Sweden)

    Gargouri Youssef

    2011-02-01

    Full Text Available Abstract Background In the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS extracts by using various solvents (hexane, chloroform-ethanol (1/1, ethyl acetate, butanol and water. We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by measuring their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA. Results The water extract exhibits the highest inhibitory effect with an IC50 = 0.22 mg/ml and interestingly no effect was observed on the digestive phospholipase A2 (group IB even at a concentration of 5 mg/ml. Antioxidant activities were also analyzed and the most active extracts were observed when using chloroform ethanol (1/1 and ethyl acetate (IC50 = 0.274 and 0.326 mg/ml, respectively. Analysis of the total phenolic content reveals that the water extract, with the best anti-PLA2 effect, was poor in phenolic molecules (2 mg GAE/g. This latter value has to be compared with the chloroform-ethanol and the ethyl acetate extracts (40 and 23.8 mg GAE/g, respectively, mostly responsible for the antioxidant activity. Conclusion A significant correlation was established between the total phenolic content and the antioxidant capacity but not with the anti PLA2 activity. Results from phytochemical screening suggest that the anti PLA2 molecules were probably catechin tannins compounds.

  16. Understanding the effect of flower extracts on the photoconducting properties of nanostructured TiO2.

    Science.gov (United States)

    Ansari, S G; Bhayana, Laitka; Umar, Ahmad; Al-Hajry, A; Al-Deyab, Salem S; Ansari, Z A

    2012-10-01

    Here we report an easy method to improve the optoelectronic properties of commercially available TiO2 nanopowder using extracts of various flowers viz. Calendula Orange (CO), Calendula Yellow (CY), Dahlia Violet (DV), Dahlia Yellow (DY), Rabbit flower (RF), Sweet Poppy (SP), Sweet Williams (SW) and their Mixed Extracts (ME). Various analysis techniques such as UV-Vis, FTIR, FESEM, XRD, and Raman spectroscopy were used to characterize for elemental, structural and morphological properties of the unmixed/mixed TiO2 nanopowder. TiO2 nanopowder was also calcined at 550 degrees C. Thick films of the these unmixed/mixed powder were printed, using conventional screen printing method, on fluorine doped tin oxide (FTO) substrate with organic binders and dried at 45 degrees C. The photoconducting properties are investigated as a function of wavelength from ultra-violet (UV) to infra-red (IR) region at a constant illumination intensity. Photocurrent gradually decreases when irradiated from UV to IR region. In case of unmixed and uncalcined TiO2, conductance decreased continuously whereas when extracts are added, a flat region of conductance is observed. The overall effect of extracts (colour pigments) is seen as an increase in the photoconductance. Highest photoconductance is observed in case of DY flower extract. Anthocyanins, present in flowers are known to have antioxidative properties and hence can contribute in photoconduction by reducing the surface adsorbed oxygen. This investigation indicates the potential use of flower extracts for dye sensitized solar cell (DSSC).

  17. Hepato and reno protective action of Calendula officinalis L. flower extract.

    Science.gov (United States)

    Preethi, Korengath Chandran; Kuttan, Ramadasan

    2009-03-01

    Flower extract of C. officinalis L. was evaluated for its protective effect against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. The activities of serum marker enzymes of liver injury like glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP) which were increased by CCl4 injection was found to be significantly reduced by the pretreatment of the flower extract at 100 and 250 mg/kg body weight. The lipid peroxidation in liver, the marker of membrane damage and the total bilirubin content in serum were also found to be at significantly low level in the extract pretreated group, indicating its protective role. The kidney function markers like urea and creatinine were significantly increased in cisplatin treated animals. However, their levels were found to be lowered in the extract pretreated groups (100 and 250 mg/kg body weight). Moreover, cisplatin induced myelosuppression was ameliorated by the extract pretreatment. Treatment with the extract produced enhancement of antioxidant enzymes--superoxide dismutase and catalase and glutathione. Results suggest a protective role of the flower extract of C. officinalis against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. Extract has been found to contain several carotenoids of which lutein, zeaxanthin and lycopene predominates. Possible mechanism of action of the flower extract may be due to its antioxidant activity and reduction of oxygen radicals.

  18. Aloe vera plant-extracted solution hydrothermal synthesis and magnetic properties of magnetite (Fe3O4) nanoparticles

    Science.gov (United States)

    Phumying, Santi; Labuayai, Sarawuth; Thomas, Chunpen; Amornkitbamrung, Vittaya; Swatsitang, Ekaphan; Maensiri, Santi

    2013-06-01

    Magnetite (Fe3O4) nanoparticles have been successfully synthesized by a novel hydrothermal method using ferric acetylacetonate (Fe(C5H8O2)3) and aloe vera plant-extracted solution. The influences of different reaction temperatures and times on the structure and magnetic properties of the synthesized Fe3O4 nanoparticles were investigated. The synthesized nanoparticles are crystalline and have particle sizes of ˜6-30 nm, as revealed by transmission electron microscopy (TEM). The results of X-ray diffraction (XRD), High resolution TEM (HRTEM) and selected area electron diffraction (SAED) indicate that the synthesized Fe3O4 nanoparticles have the inverse cubic spinel structure without the presence of any other phase impurities. The hysteresis loops of the Fe3O4 nanoparticles at room temperature show superparamagnetic behavior and the saturation magnetization of the Fe3O4 samples increases with increasing reaction temperature and time.

  19. Antimicrobial Activity of Extracts from Leaves, Stems and Flowers of Euphorbia macroclada against plant pathogenic fungi

    Directory of Open Access Journals (Sweden)

    K. Al-Mughrabi

    2003-12-01

    Full Text Available Extracts drawn from dried and powdered flowers, stems and leaves of Euphorbia macroclada with some organic solvents were tested for antimicrobial effect against the fungi Verticillium dahliae, Fusarium oxysporum, Rhizopus stolonifer, Penicillium italicum, Rhizoctonia solani, Alternaria solani, Stemphylium solani, Cladosporium sp., Mucor sp., and Pythium sp. The strongest inhibitory effect of the extracts was observed against R. solani, V. dahliae, F. oxysporum, Pythium sp. and R. stolonifer. The weakest effect was against A. solani. Extracts from the stems had a stronger inhibitory effect than those from the flowers or leaves. Butanol was the best solvent to extract antimicrobial compounds from leaves, stems and flowers and was superior to chloroform, water and petroleum ether. Results clearly indicate that E. macroclada is a promising source of antimicrobial compounds.

  20. Antispasmodic effects of Citrus aurantium flowers aqueous extract on uterus of non-pregnant rats

    OpenAIRE

    Akram Ahangarpour; Ali Akbar Oroojan; Ashraf Amirzargar; Maryam Ghanavati

    2011-01-01

    Background: Citrus aurantium is a small citrus tree, with scented white flowers. The C. aurantium is used in Asian herbal medicine primarily to treat digestive problems.Objective: The goal of this study is to investigate the effect of C. aurantium flower's aqueous extract on uterine contraction in presence of some known uterus stimulants.Materials and Methods: In experimental study 30 virgin Wistar rats 200-300gr were obtained. After laparatomy, a piece of Uterus was dissected out and mounted...

  1. Antioxidant, antibacterial and in vivo dermal wound healing effects of Opuntia flower extracts.

    Science.gov (United States)

    Ammar, Imene; Bardaa, Sana; Mzid, Massara; Sahnoun, Zouheir; Rebaii, Tarak; Attia, Hamadi; Ennouri, Monia

    2015-11-01

    Opuntia ficus-indica flowers are used for various medicinal purposes. The aims of the present investigation were to evaluate biological properties of O. ficus-indica flowers extracts and to investigate its antioxidant and antibacterial activities and its ability to enhance wound healing. The wound healing activity of the mucilaginous and methanol extracts of O. ficus-indica flowers were assessed using excision wound model in rats. After thirteen days of treatment by both extracts, a beneficial effect on cutaneous repair was observed as assessed by the acceleration of wound contraction and remodeling phases. Histopathological studies of the granulation tissue indicated that the derma is properly arranged with the Opuntia flowers extract, compared with the control group. The mucilage extract was more effective than the methanol extract, but both showed significant results compared with the control. Such investigation was supported by the efficiency of the methanolic and mucilage extract as antimicrobial and antioxidant. Indeed, the extracts showed a potential antioxidant activity determined by different test systems, namely DPPH radicals scavenging activity, trolox equivalent antioxidant capacity, reducing power, β-carotene bleaching assay and metal chelating activity and exhibited significant antibacterial activity against almost all tested bacteria. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Aloe vera

    Energy Technology Data Exchange (ETDEWEB)

    Klein, A.D.; Penneys, N.S.

    1988-04-01

    We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials. 27 references.

  3. Aloe vera

    International Nuclear Information System (INIS)

    Klein, A.D.; Penneys, N.S.

    1988-01-01

    We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials. 27 references

  4. Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na{sup 99m}TcO{sub 4}) in rats

    Energy Technology Data Exchange (ETDEWEB)

    Holanda, Cecilia Maria de Carvalho Xavier [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. of Microbiology and Parasitology. Experimental Radiobiology and Antiparasitic Assays Lab.], e-mail: cechol@ufrnet.br; Costa, Monique Batista da; Silva, Natalia Chilinque Zambao da; Silva Junior, Mauricio Ferreira da; Barbosa, Vanessa Santos de Arruda; Silva, Roseane Pereira da [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil); Medeiros, Aldo da Cunha [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Nucleus of Experimental Surgery

    2009-07-01

    Purpose: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na{sup 99m}TcO{sub 4} and laboratorial parameters in Wistar rats. Methods: twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na{sup 99m}TcO{sub 4} by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (% ATI/g) was determined. Biochemical dosages were performed. Results: there was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p<0.05). Conclusion: The aqueous extract of Aloe vera facilitated the uptake of Na{sup 99m}TcO{sub 4} in organs of rats and it was responsible to a high increase of levels of AST and ALT. (author)

  5. Ultrasound-assisted extraction of phenolic antioxidants from Acacia confusa flowers and buds.

    Science.gov (United States)

    Tung, Yu Tang; Chang, Wei Chun; Chen, Ping Sheng; Chang, Tzu Cheng; Chang, Shang Tzen

    2011-04-01

    Acacia confusa Merr. (Leguminosae), a species native to Taiwan, is widely distributed on the hills and lowlands of Taiwan, and has been used in traditional medicines. In this study, the application of ultrasound-assisted extraction was used to extract the phenolic compounds from A. confusa flowers and buds for the first time. Among the extraction methods, it can significantly enhance the contents of phenolic compounds and antioxidant activities in A. confusa flower and bud extracts using ultrasound-assisted extraction (10  min×12 times). Considering both the solvent consumption and the time needed for extraction, ultrasound-assisted extraction was found to be the most practical approach for the rapid and efficient extraction of bioactive phenolic constituents. In addition, gallic acid, myricitrin-3-rhamnoside, quercitrin-3-rhamnoside, europetin-3-rhamnoside, kaempferol-3-rhamnoside, rhamnetin-3-glucoside, and rhamnetin-3-rhamnoside were also quantified in different extracts by RP-HPLC. It is clear that ultrasound-assisted extraction is an efficient method for extracting phenolic compounds from A. confusa flowers and buds. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. flowers

    African Journals Online (AJOL)

    Administrator

    2011-06-08

    Jun 8, 2011 ... Infrastructure Construction Project of Jiangsu Province. (BM2010590). REFERENCES. Ammar AH, Zagrouba F, Romdhane M (2010). Optimization of operating conditions of Tunisian myrtle (Myrtus communis L.) essential oil extraction by a hydrodistillation process using a 2(4) complete factorial design.

  7. Investigation on structural properties of M-type strontium hexaferrite synthesized in presence of neem and aloe-vera plant leaves extract

    Science.gov (United States)

    Solanki, Neha; Jotania, Rajshree B.

    2017-05-01

    M-type strontium hexaferrite powder samples were synthesized using a green synthesis route with and without presence of Aloe vera and Neem leaves extract. The dry brownish precursors of strontium hexaferrite were recovered from a mixed solution of metal salts and leaves extract, heated at 100 °C. The obtained precursors were pre-heated at 500 °C for 4 hrs. followed by final heating at 950 °C for 4 hrs. in a muffle furnace to obtain SrFe12O19 hexaferrite powder. The obtained SrFe12O19 hexaferrite powder samples characterized at room temperature in order to check phase purity and structural properties. XRD analysis confirms that samples prepared without and with Aloe vera leaves extract (heated at 950 °C for 4 hrs.) show formation of α-Fe2O3 and M-phase; while the sample prepared in presence of Neem leaves extract (heated at 950 °C for 4 hrs.) show formation of mono phase of strontium hexaferrite. Lattice parameter (a) and cell volume (V) are found to increase in the samples prepared in presence of Aloe vera and Neem leaves extract.

  8. The Effects of Punica granatum Flower Extract on Skin Injuries Induced by Burn in Rats

    Science.gov (United States)

    Nasiri, Ebrahim; Akbari, Jafar; Azadbakht, Mohammad; Azizi, Soheil

    2017-01-01

    Background. We compared the efficacy of P. granatum (P) flower extract with that of silver sulfadiazine (SSD) for treating thermal burn injuries in rats. Methods. Ten Wistar rats in each group were topically given base cream, normal saline, cream containing 1% SSD, or creams containing 5% or 10% Punica granatum flower extract. The treatments were administered once daily until complete wound healing was observed. The wound area and healing time were assessed. In addition, percentage wound contraction and histopathological characteristics such as neovascularization and collagen formation were determined. The tannin content in P. granatum extract was determined. Results. The decrease in the average size of wounds on day 15 of the treatment was higher in rats treated with creams containing P. granatum extract than in rats treated with cream containing SSD (2.8 ± 0.9 cm2 versus 8.4 ± 3.2 cm2). The wounds completely healed on day 25 of the treatment in rats treated with creams containing P. granatum flower extract compared with those in rats treated with the other agents. Conclusion. These results indicated that P. granatum flower extract promoted wound healing in rats and could be used for managing burn injuries. PMID:28203250

  9. The Effects of Punica granatum Flower Extract on Skin Injuries Induced by Burn in Rats

    Directory of Open Access Journals (Sweden)

    Ebrahim Nasiri

    2017-01-01

    Full Text Available Background. We compared the efficacy of P. granatum (P flower extract with that of silver sulfadiazine (SSD for treating thermal burn injuries in rats. Methods. Ten Wistar rats in each group were topically given base cream, normal saline, cream containing 1% SSD, or creams containing 5% or 10% Punica granatum flower extract. The treatments were administered once daily until complete wound healing was observed. The wound area and healing time were assessed. In addition, percentage wound contraction and histopathological characteristics such as neovascularization and collagen formation were determined. The tannin content in P. granatum extract was determined. Results. The decrease in the average size of wounds on day 15 of the treatment was higher in rats treated with creams containing P. granatum extract than in rats treated with cream containing SSD (2.8±0.9 cm2 versus 8.4±3.2 cm2. The wounds completely healed on day 25 of the treatment in rats treated with creams containing P. granatum flower extract compared with those in rats treated with the other agents. Conclusion. These results indicated that P. granatum flower extract promoted wound healing in rats and could be used for managing burn injuries.

  10. Antispasmodic effects of Citrus aurantium flowers aqueous extract on uterus of non-pregnant rats.

    Science.gov (United States)

    Ahangarpour, Akram; Oroojan, Ali Akbar; Amirzargar, Ashraf; Ghanavati, Maryam

    2011-01-01

    Citrus aurantium is a small citrus tree, with scented white flowers. The C. aurantium is used in Asian herbal medicine primarily to treat digestive problems. The goal of this study is to investigate the effect of C. aurantium flower's aqueous extract on uterine contraction in presence of some known uterus stimulants. In experimental study 30 virgin Wistar rats 200-300gr were obtained. After laparatomy, a piece of Uterus was dissected out and mounted in an organ bath (10ml) containing De Jalon (29°C) and contracted by KCl (60mM), oxytocin (10mU/ml) and barium chloride (4mM) then the effect of C. aurantium flower's aqueous extract (1-8 mg/ml) on the uterine contractions was investigated.μμthe role of β-adrenoceptors, opioid receptors were evaluated. Cumulative concentrations of the extract (1-8 mg/ml) decreased KCl, oxytocin and barium chloride induced uterine contractions, dose-dependently (paurantium flower's aqueous extract was unaffected on incubation the tissue with propranolol and naloxone. It seems that the extract induced antispasmodic effect mainly via calcium influx blockade. However, neither β-adrenoceptors nor opioid receptors were involved. Since the extract has antispasmodic effect on uterus contraction therefore we can suggest that more study will be necessary to relief dysmenorrheal.

  11. The Effects of Punica granatum Flower Extract on Skin Injuries Induced by Burn in Rats.

    Science.gov (United States)

    Nasiri, Ebrahim; Hosseinimehr, Seyed Jalal; Akbari, Jafar; Azadbakht, Mohammad; Azizi, Soheil

    2017-01-01

    Background . We compared the efficacy of P. granatum (P) flower extract with that of silver sulfadiazine (SSD) for treating thermal burn injuries in rats. Methods . Ten Wistar rats in each group were topically given base cream, normal saline, cream containing 1% SSD, or creams containing 5% or 10% Punica granatum flower extract. The treatments were administered once daily until complete wound healing was observed. The wound area and healing time were assessed. In addition, percentage wound contraction and histopathological characteristics such as neovascularization and collagen formation were determined. The tannin content in P. granatum extract was determined. Results . The decrease in the average size of wounds on day 15 of the treatment was higher in rats treated with creams containing P. granatum extract than in rats treated with cream containing SSD (2.8 ± 0.9 cm 2 versus 8.4 ± 3.2 cm 2 ). The wounds completely healed on day 25 of the treatment in rats treated with creams containing P. granatum flower extract compared with those in rats treated with the other agents. Conclusion . These results indicated that P. granatum flower extract promoted wound healing in rats and could be used for managing burn injuries.

  12. Pharmacological evaluation for anti-asthmatic and anti-inflammatory potential of Woodfordia fruticosa flower extracts.

    Science.gov (United States)

    Hiralal Ghante, Mahavir; Bhusari, Kishore P; Duragkar, Nandkishore J; Ghiware, Nitin B

    2014-07-01

    Woodfordia fruticosa Kurz. (Lythraceae) flowers are ethnopharmacologically acclaimed in the Indian medicinal system to treat asthma. To evaluate W. fruticosa flower extracts for anti-asthmatic effect. Ethyl acetate, acetone, methanol, and hydro-alcohol extracts of W. fruticosa flowers were obtained successively and standardized. Ability of extracts to stabilize free radicals and compound-48/80-induced mast cell degranulation was evaluated. In vitro anti-inflammatory potential of extracts at 100 and 200 µg/ml by membrane stabilization and in vivo inhibition of rat paw edema up to 5 h (100 and 200 mg/ml; p.o.) was evaluated. In vitro bronchorelaxant effect was examined against histamine- and acetylcholine (1 µg/ml; independently)-induced guinea pig tracheal contraction. Extracts were evaluated for bronchoprotection (in vivo) ability against 0.1% histamine- and 2% acetylcholine-induced bronchospasm in guinea pigs at 100 and 200 mg/ml; p.o. Standardization studies revealed that the methanol extract exhibited highest polyphenolic (62.66 GAE), and flavonoid (6.32 RE) content and HPLC fingerprinting confirmed the presence of gallic acid (Rt 1.383). IC50 values for DPPH scavenging and metal chelation by the methanol extract were 40.42 and 31.50 µg/ml. Methanol and ethyl acetate extracts at 100 µg/ml exhibited 06.52 and 07.12% of histamine release. Methanol, ethyl acetate, and hydro alcohol extracts at 200 mg/kg demonstrated 32.73, 29.83, 26.75% and 32.46, 9.38, 26.75% inhibition of egg albumin and carrageenan-induced inflammation, respectively. Methanol extract exhibited 100% bronchorelaxation and 48.83% bronchoprotection. Woodfordia fruticosa flower (WFF) extracts exhibited anti-asthmatic effect by demonstrating bronchoprotection, bronchorelaxation, anti-inflammatory, antioxidant, and mast cell stabilization ability.

  13. The effects of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid- induced gastric ulcer in male rats

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    Zakieh Keshavarzi

    2014-02-01

    Full Text Available Objective: Gut–brain axis (GBA is very important in creation and modulation of gastrointestinal problems. Aloe vera gel has gastroprotective properties. The purpose of this study was to evaluate the effect of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid gastric ulcer induction. Materials and Methods: Gastric ulcer was induced by injection of 20% acetic acid into the subserosal layer in male rats. Rats were randomly assigned into three groups: intact group, gastric ulcer group and Aloe vera group (treatment with Aloe vera following gastric ulcer induction. The acid levels and brain and intestinal water content of each sample were measured eight days after the gastric ulcer induction. Results: Gastric acid levels were significantly decreased in Aloe vera group when compared with gastric ulcer group (p

  14. COMPARATIVE IN VITRO ANTHELMINTIC AND PHYTOCHEMICAL EVALUATION OF METHANOLIC AND PETROLEUM ETHER EXTRACTS OF WOODFERDIA FRUTICOSA (L). FLOWER

    OpenAIRE

    Sengupta Rupa

    2013-01-01

    The present study aims at preliminary phytochemical investigation and anthelmintic activity of dried flowers of Woodfordia fruticosa (L). The dried flowers were extracted individually with methanol and petrolium ether. The extracts were evaluated for phytochemical studies. Preliminary screening of the flowers of Woodfordia fruticosa revealed the presence of carbohydrates, tannins and glycosides in major quantities, phenols in moderate quantities and anthraquinones and flavonoids in minor quan...

  15. Trifolium pratense and T. repens (Leguminosae: Edible Flower Extracts as Functional Ingredients

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    Rosa Tundis

    2015-08-01

    Full Text Available Trifolium pratense (red clover and T. repens (white clover edible flowers were investigated for their chemical profile and health properties. The total phenols and flavonoids contents were evaluated. Quercetin, kaempferol, luteolin, rutin, and myricetin were used as markers and quantified by HPLC. The antioxidant effects were investigated by using different in vitro assays. Moreover, α-amylase, α-glucosidase and lipase inhibitory activities were evaluated. T. repens flowers extract showed a good radical scavenging activity in both DPPH and ABTS tests with IC50 values of 10.3 and 21.4 μg/mL, respectively. White clover extract demonstrated promising α-amylase and lipase inhibitory activities with IC50 values of 25.0 and 1.3 μg/mL, respectively. The obtained results support the use of Trifolium flowers as healthy food ingredients.

  16. STUDY ON LANDSLIDE DISASTER EXTRACTION METHOD BASED ON SPACEBORNE SAR REMOTE SENSING IMAGES – TAKE ALOS PALSAR FOR AN EXAMPLE

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    D. Xue

    2018-04-01

    Full Text Available In this paper, sequence ALOS PALSAR data and airborne SAR data of L-band from June 5, 2008 to September 8, 2015 are used. Based on the research of SAR data preprocessing and core algorithms, such as geocode, registration, filtering, unwrapping and baseline estimation, the improved Goldstein filtering algorithm and the branch-cut path tracking algorithm are used to unwrap the phase. The DEM and surface deformation information of the experimental area were extracted. Combining SAR-specific geometry and differential interferometry, on the basis of composite analysis of multi-source images, a method of detecting landslide disaster combining coherence of SAR image is developed, which makes up for the deficiency of single SAR and optical remote sensing acquisition ability. Especially in bad weather and abnormal climate areas, the speed of disaster emergency and the accuracy of extraction are improved. It is found that the deformation in this area is greatly affected by faults, and there is a tendency of uplift in the southeast plain and western mountainous area, while in the southwest part of the mountain area there is a tendency to sink. This research result provides a basis for decision-making for local disaster prevention and control.

  17. Influence of ethanolic extract of Jasminum grandflorum linn flower on wound healing activity in rats.

    Science.gov (United States)

    Nayak, B S; Mohan, Krishna

    2007-01-01

    The influence of flower extract of Jasminum grandiflorum was studied for its wound healing activity at a dose of 250 mg/kg body weight, using excision and dead space wound models in rats. The animals were divided into three groups in excision wound model, the controls (n=10) were treated with 0.25% CM cellulose, reference standard (n=10) were treated with sulfathiazole ointment and the experimental (n=10) were treated with extract of J. grandiflorum flower till complete epithelialization. The animals in dead space wound models were divided into two groups, controls were given plain drinking water and the experimental animals were administered with extract orally for 10 days. The extract treated wounds were found to epithelize faster as compared to controls. Extract-treated rats exhibited 65% reduction in the wound area when compared to controls (54%). The wet and dry granulation tissue weight, and hydroxyproline content in a dead space wound model increased significantly (P day 10 from the extract-treated group showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells when compared to controls which showed inflammatory cells, scanty collagen fibres and fibroblasts. The. demonstration of increased rate of wound contraction together with the biochemical and histological findings suggest the use of J. grandiflorum flower extract in the management of wound healing.

  18. Effect of Thai saraphi flower extracts on WT1 and BCR/ABL protein ...

    African Journals Online (AJOL)

    In this study, the cytotoxic effects of crude ethanolic and fractional extracts including hexane, ethyl acetate, and methanol fractions from M. siamensis flowers were investigated in order to determine their effect on WT1 expression in Molt4 and K562 cells and Bcr/Abl expression in K562 cells. Materials and Methods: The ...

  19. Flower extract as an improvised indicator in acid – base titration ...

    African Journals Online (AJOL)

    Different flowers were collected and the extracts were tested for indicator properties in acidic and basic solutions. The results showed different colour changes in alkaline and colourless in acid solutions. When used in acid-base titration, the end points colours and the average titre values obtained matched with that of ...

  20. Antioxidant Activities of Caragana sinica Flower Extracts and Their Main Chemical Constituents

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    Zhi-Gang Tai

    2010-09-01

    Full Text Available The edible flowers of Caragana sinica are used in China a kind of health-promoting vegetable. In this study, the antioxidant activities of its ethanol extract, as well as its petroleum ether, ethyl acetate, n-butanol and water fractions, were evaluated through in vitro model systems including the DPPH, FRAP and β-carotene bleaching methods. Among the C. sinica flower extracts the ethyl acetate fraction was the most effective. Correlation analysis suggested that flavonoids might be the major contributors to the high antioxidant activity of this flower. Six flavonoids including quercetin (1, isoquercitrin (2, rutin (3, quercetin-3′-O-methyl-3-O-α-L-rhamnopyranosyl (1→6-β-D-glucopyranoside (4, typhaneoside (5 and quercetin-3-O-β-D-glucopyranosyl(1→2[α-L-rhamnopyranosyl (1→6]-β-D-glucopyranoside (6, were isolated from this flower for the first time. Their contents were determined by a HPLC method. Compounds 3 and 4 were found to be the major flavonoids, with concentrations of 1.20 ± 0.07 and 3.94 ± 0.12 mg/g dry sample, respectively. These results demonstrated that the C. sinica flowers may be valuable natural antioxidant sources and are potentially applicable in the health food industry.

  1. Green Synthesis of Silver Nanoparticles using Extract of Pinus merkusii Jungh & De Vriese Cone Flower

    Science.gov (United States)

    Azkiya, N. I.; Masruri, M.; Ulfa, S. M.

    2018-01-01

    The paper studies recent application of cone flower waste from Pinus merkusii Jungh & De Vriese for an environmentally unclear method for synthesis silver nanoparticle. Phytochemical characterization using iron trichloride solution showed the extract of Pinus merkusii cone flower contains of phenolic group of secondary metabolite. This group acts as both reducing and stabilizing agents. For the synthesis of silver nanoparticle, solution of silver nitrate is added to the extract at 60°C. The effect of extract concentration (5-20%) and time reaction (15-60 min) is investigated. The formation of silver nanoparticle is confirmed by the color change from yellowish to brown. Meanwhile, UV-Vis characterization of silver nanoparticle in extract 20% and 60 min reaction showed surface plasmon resonance (SPR) at 431 nm, and transmission electron microscope (TEM) revealed the particle size range in between 8 and 23 nm with a spherical in shape.

  2. Determination of total lipids and characterization of marigold flower extracts (Calendula officinalis

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    Novković Vesna M.

    2005-01-01

    Full Text Available Bioactive extracts from marigold flower are important ingredients for parapharmaceutical and cosmetic preparations. Their antiflogistic holeretic.antimicrobic, antidermatic and anticancer effects are due to the presence of flavonoids, carotenoids, etheric oils, and terpenoids. This study presents the results of spectrophotometric investigation for the overall carotene content calculated as (3-caroten (442 nm, visual and physico-chemical characteristics according to Ph.Jug. V in oil extracts of marigold flower obtained by maceration (room temperature and 70°C and percolation (room temperature with olive oil and sunflower oil by original procedures.The largest content of (3-carotene (57.5 mg/kg of oil extracts is in the oil extract obtained by maceration for 72 hours with olive oil (solvomodulus 1:5 m/m at room temperature.

  3. Bioactivity-guided fractionation to identify β-glucuronidase inhibitors in Nymphaea pubescens flower extract

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    Jayashree Acharya

    2016-12-01

    Full Text Available The plant Nymphaea pubescens Willd. (Family: Nymphaeaceae is edible having medicinal importance. The objective of the study was to analyze the potential hepatoprotective properties of the flowers and pedicels of N. pubescens by inhibiting the enzyme β-glucuronidase. Crude methanol extracts of flower and pedicel as well as chloroform, ethyl acetate, and aqueous fractions of the flower extract were tested for their activities against the enzyme in vitro. The extracts and the fractions were analyzed by GC–MS to identify metabolites present in them. Flower (IC50 value = 270.27 ± 4.67 μg/ml and pedicel (IC50 value = 868.46 ± 28.21 μg/ml extracts have shown to inhibit the β-glucuronidase activity. Chloroform (IC50 value = 147.16 ± 6.68 μg/ml, ethyl acetate (IC50 value = 183.94 ± 2.37 μg/ml, and aqueous (IC50 value = 339.43 ± 5.34 μg/ml fractions showed significantly stronger activity than that of silymarin (IC50 value = 792.62 ± 10.01 μg/ml, the known inhibitor of the enzyme. GC–MS-based analysis of the flower extract and solvent fractions led to the identification of kaempferol having 79-fold stronger activity than that of silymarin, IC50 value of kaempferol being 10.44 ± 0.084 μg/ml or 0.0037 mM ± 0.0001.

  4. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats

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    Pinto Pereira Lexley

    2006-12-01

    Full Text Available Abstract Background Catharanthus roseus L (C. roseus has been used to treat a wide assortment of diseases including diabetes. The objective of our study was to evaluate the antimicrobial and wound healing activity of the flower extract of Catharanthus in rats. Methods Wound healing activity was determined in rats, after administration (100 mg kg-1 day-1 of the ethanol extract of C. roseus flower, using excision, incision and dead space wounds models. The animals were divided into two groups of 6 each in all the models. In the excision model, group 1 animals were topically treated with carboxymethyl cellulose as placebo control and group 2 received topical application of the ethanol extract of C. roseus at a dose of 100 mg/kg body weight/day. In an incision and dead space model group 1 animals were given normal saline and group 2 received the extract orally at a dose of 100 mg kg-1 day-1. Healing was assessed by the rate of wound contraction, period of epithelization, tensile strength (skin breaking strength, granulation tissue weight, and hydoxyproline content. Antimicrobial activity of the flower extract against four microorganisms was also assessed Results The extract of C. roseus significantly increased the wound breaking strength in the incision wound model compared with controls (P Pseudomonas aeruginosa and Staphylococcus aureus demonstrated sensitivity to C. roseus Conclusion Increased wound contraction and tensile strength, augmented hydroxyproline content along with antimicrobial activity support the use of C. roseus in the topical management of wound healing.

  5. Anthocyanin Characterization of Pilot Plant Water Extracts of Delonix regia Flowers

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    Emile M. Gaydou

    2008-06-01

    Full Text Available Following the development of new applications of pilot plant scale extraction and formulation processes for natural active bioproducts obtained from various underutilized tropical plants and herbs, we have manufactured water-extracts from Delonix regia flowers, grown in Ivory Coast. These extracts, which contain polyphenols, are traditionally home made and used as healthy bioproducts. They are reddish-coloured due to the presence of anthocyanins. The three major anthocyanins in these extracts have been characterized. The molecular structures were confirmed by LC-SM analysis. Amongst them, two are described for the first time in Delonix regia.

  6. Automated Control of the Organic and Inorganic Composition of Aloe vera Extracts Using (1)H NMR Spectroscopy.

    Science.gov (United States)

    Monakhova, Yulia B; Randel, Gabriele; Diehl, Bernd W K

    2016-09-01

    Recent classification of Aloe vera whole-leaf extract by the International Agency for Research and Cancer as a possible carcinogen to humans as well as the continuous adulteration of A. vera's authentic material have generated renewed interest in controlling A. vera. The existing NMR spectroscopic method for the analysis of A. vera, which is based on a routine developed at Spectral Service, was extended. Apart from aloverose, glucose, malic acid, lactic acid, citric acid, whole-leaf material (WLM), acetic acid, fumaric acid, sodium benzoate, and potassium sorbate, the quantification of Mg(2+), Ca(2+), and fructose is possible with the addition of a Cs-EDTA solution to sample. The proposed methodology was automated, which includes phasing, baseline-correction, deconvolution (based on the Lorentzian function), integration, quantification, and reporting. The NMR method was applied to 41 A. vera preparations in the form of liquid A. vera juice and solid A. vera powder. The advantages of the new NMR methodology over the previous method were discussed. Correlation between the new and standard NMR methodologies was significant for aloverose, glucose, malic acid, lactic acid, citric acid, and WLM (P vera.

  7. The influence of Aloe vera and xenograft XCB toward of bone morpho protein 2 BMP2 expression and amount of osteoblast of alveolar bone induced into tooth extraction sockets Cavia cobaya

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    Utari Kresnoadi

    2014-12-01

    Full Text Available Tooth extraction can cause inflammation leading to alveolar ridge resorption. In addition, prominent ridge has crucial role for making denture su-ccessfully. Thus, socket preservation is needed to prevent greater alveolar ridge resorption. An innovative material, a combination of Aloe vera and xe-nograft (XCB, is then considered as a biogenic stimulator that can reduce inflammation, as a result, the growth of alveolar bone is expected to be impro-ved. This research is aimed to prove whether the mixture of Aloe vera and xenograft can stimulate BMP2 and increase osteoblasts. Forty-eight Cavia co-baya animals were divided into eight groups each of which consisted of six animals. The mandibular incisors of those Cavia cobaya animals were then extracted and filled with PEG as Group Control, XCB as Group XCB, Aloe vera as Group Aloe vera, and a combination of Aloe vera +XCB as Group Aloe vera +XCB. Next, the first four groups were sacrificed seven days after extraction, and the second four groups were sacrificed 30 days after extrac-tion. And then, immunohistochemical and histopathology examinations were conducted to examine BMP2 expression and osteoblasts. Based on the re-sult known that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblasts. It can be concluded that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblast cel . It can be used as an alternative material to increase the growth of alveolar bone after extraction.

  8. A Potent Staphylococcus Aureus Growth Inhibitor Of A Dried Flower Extract Of Pinus Merkusii Jungh & De Vriese And Copper Nanoparticle

    Science.gov (United States)

    Masruri, Masruri; Norani Pangestin, Dinna; Mariyah Ulfa, Siti; Riyanto, Slamet; Srihardyastutie, Arie; Farid Rahman, Moh.

    2018-01-01

    The paper report antibacterial activity of flower extract from Pinus merkusii Jungh Et De Vriese and its mixture with copper nanoparticle on Staphylococcus aureus. This finding revealed the potency of pine forestry waste to overcome a bacterial-resistance problem on some commercially antibiotics. The extract was prepared by hot water extraction of a dried powder of pine flower. Copper nanoparticle was synthesized following “green synthesis technique” using phenolic-rich extract of pine’s flower as a reduction and capping agent. In short, a mixture of pine’s flower extract and copper nanoparticle importantly was able to inhibit the growth of Staphylococcus aureus four times higher than that using water extract.

  9. Antispasmodic effects of Citrus aurantium flowers aqueous extract on uterus of non-pregnant rats

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    Akram Ahangarpour

    2011-01-01

    Full Text Available Background: Citrus aurantium is a small citrus tree, with scented white flowers. The C. aurantium is used in Asian herbal medicine primarily to treat digestive problems.Objective: The goal of this study is to investigate the effect of C. aurantium flower's aqueous extract on uterine contraction in presence of some known uterus stimulants.Materials and Methods: In experimental study 30 virgin Wistar rats 200-300gr were obtained. After laparatomy, a piece of Uterus was dissected out and mounted in an organ bath (10ml containing De Jalon (29°C and contracted by KCl (60mM, oxytocin (10mU/ml and barium chloride (4mM then the effect of C. aurantium flower's aqueous extract (1-8 mg/ml on the uterine contractions was investigated. Uterus was separately incubated with propranolol (1M, naloxone (1M and the role of β-adrenoceptors, opioid receptors were evaluated.Results: Cumulative concentrations of the extract (1-8 mg/ml decreased KCl, oxytocin and barium chloride induced uterine contractions, dose-dependently (p<0.001. C. aurantium flower's aqueous extract was unaffected on incubation the tissue with propranolol and naloxone.Conclusion: It seems that the extract induced antispasmodic effect mainly via calcium influx blockade. However, neither β-adrenoceptors nor opioid receptors were involved. Since the extract has antispasmodic effect on uterus contraction therefore we can suggest that more study will be necessary to relief dysmenorrheal

  10. Antimicrobial Cream Formulated with Supercritical Carbon Dioxide Extract of Tuberose Flowers Arrests Growth of Staphylococcus aureus.

    Science.gov (United States)

    Ghosh, Probir Kumar; Bhattacharjee, Paramita; Das, Satadal

    2016-01-01

    Antimicrobial potency of herbal extracts is well known. The review of patents and research articles revealed that several herbal extracts have been employed in the formulation of topical products such as creams, exclusive of the cream reported in the present study. 0ur previous study has established antimicrobial potency of supercritical carbon dioxide extracts of tuberose flowers, better known for its sweet fragrance. The present work focuses on formulating a topical antimicrobial herbal cream with methyl eugenol (principal antimicrobial compound) rich - supercritical carbon dioxide extract of tuberose flowers, having good combination of phytochemical and antimicrobial potencies. Supercritical carbon dioxide parameters such as temperature, pressure and time were optimized using full factorial experimental design to obtain methyl eugenol-rich extracts. A cream was formulated using the extract having the best combination of phytochemical and antimicrobial potencies and was assayed further for in vitro antimicrobial potency; physiochemical and sensory properties. Two commercial antimicrobial cream samples were used as reference samples in the study. The extract obtained at 40°C, 10 MPa, 135 min at 1 L min-1 flow rate of gaseous C02 showed the best combination of phytochemical and antimicrobial potencies and was used for formulation of herbal creams. The cream formulated with 5% w/w of extract arrested growth of the common human skin pathogen Staphylococcus aureus and showed stable physiochemical properties and high sensory appeal for a year. The cream could be considered as a 'finished herbal product&' in compliance with the World Health 0rganization guidelines.

  11. Antibacterial activity of ethanol extract and fractions obtained from Taraxacum mongolicum flower

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    H. Qiao

    2014-10-01

    Full Text Available Background and objectives: Resistance towards reveling antibiotics has captured great interest in evaluating the antimicrobial properties of the natural plants. Taraxacum mongolicum is widely used as a folklore medicinal plant for its diuretic, antirheumatic and anti-inflammatory properties. Though there are some reports on antimicrobial properties of Taraxacum mongolicum, studies on antibacterial abilities of its flower are limited and it was decided to evaluate the antibacterial properties of the flowers in the present study. Methods: The antibacterial properties of ethanol extract of Taraxacum mongolicum flower, and its fractions (petroleum ether, ethyl acetate (ET, and aqueous fractions were examined through agar disc diffusion method, and the minimum inhibitory concentration (MIC was determined. Four Gram-negative and two Gram-positive bacteria were used in the study. Results: The antibacterial test results showed that the ET fraction strongly inhibited the growth of all of the microorganisms, especially Pseudomonas aeruginosa and Bacillus subtilis (with MIC values of 125 μg/mL and 62.5 μg/mL, respectively, whereas the ethanol extract and the other two fractions demonstrated moderate and weak activities, respectively. Conclusion: The ET fraction obtained from Taraxacum mongolicum flowers presented high antibacterial activity and might be suggested for use as a natural preservative ingredient in pharmaceutical industries.

  12. Cardioprotective effect of hydroalcoholic extract of Tecoma stans flowers against isoproterenol induced myocardial infarction in rats

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    Shanmukha Ittagi

    2014-02-01

    Full Text Available Objective: To investigate the cardioprotective effect of 70% ethanolic extract of Tecoma stans (T. stans flowers against isoproterenol-induced myocardial infarction in rat myocardium. Methods: Wister rats were pretreated with 70% ethanolic extract of T. stans flowers (250 and 500 mg/kg orally for 14 d and then intoxicated with isoproterenol [200 mg/(kg · day, s.c.] for 2 consecutive d. The biochemical markers for myocardial infarction such as alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatinine kinase, total cholesterol, triglycerides, low density lipoproteins and high density lipoproteins were determined. In addition the antioxidant status on heart tissue is also evaluated by testing the activities of antioxidant enzymes such as lipid peroxidation, superoxide dismutase, reduced glutathione and catalase. Results: The results indicated that pretreatment with 70% ethanolic extract of T. stans flowers prevented fall in antioxidants and retarded elevation of cardiac damage markers in isoproterenol treated rats, significantly. In addition, these findings were evidently supported by the remarkable protection revealed in the histopathological studies, even GC-MS analysis data also substantiated out investigation. Conclusions: It was concluded that, in addition to poly phenolics, some of the phyto fragments found during GC-MS analysis might also contributed to the cardiac protection offered by the extract.

  13. Aloe Vera

    Science.gov (United States)

    ... month drinking water toxicity study in F344 rats . Food and Chemical Toxicology . 2013;57:21-31. Yang HN, Kim DJ, Kim YM, et al. Aloe-induced toxic hepatitis . Journal of Korean Medical Science. 2010;25(3):492-495. This ...

  14. Optimization of Ultrasound-Assisted Extraction of Natural Antioxidants from the Osmanthus fragrans Flower.

    Science.gov (United States)

    Li, An-Na; Li, Sha; Li, Ya; Xu, Dong-Ping; Li, Hua-Bin

    2016-02-18

    An ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the Osmanthus fragrans flower. The effect of UAE on antioxidant activity of the extract from the Osmanthus fragrans flower was studied using a Trolox equivalent antioxidant capacity (TEAC) assay. Optimization conditions were firstly determined using a single-factor experiment, and response surface methodology was then used to evaluate interaction of several experimental parameters. Analysis of the coefficient of determination showed that second-order polynomial models produced a highly satisfactory fitting of the experimental data with regard to TEAC values (R² = 0.9829, p extraction for 35.2 min at 59.4 °C. Under these conditions, the maximum TEAC value was 584.9 ± 6.0 μmol Trolox/g DW, which was higher than those obtained by the conventional extracting method (486.4 ± 12.6 μmol Trolox/g DW) and the Soxhlet extraction method (339.1 ± 16.2 μmol Trolox/g DW). The crude extract obtained could be used either as a food additive or in pharmaceuticals for the prevention and treatment of diseases caused by oxidative stress.

  15. Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

    International Nuclear Information System (INIS)

    Holanda, Cecilia Maria de Carvalho Xavier; Medeiros, Aldo da Cunha

    2009-01-01

    Purpose: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na 99m TcO 4 and laboratorial parameters in Wistar rats. Methods: twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na 99m TcO 4 by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (% ATI/g) was determined. Biochemical dosages were performed. Results: there was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p 99m TcO 4 in organs of rats and it was responsible to a high increase of levels of AST and ALT. (author)

  16. Anti sickling potential of Aloe vera extract IV: Effects of acidic, basic ...

    African Journals Online (AJOL)

    The effects of acidic, basic and neutral metabolites from A. vera extract on the sickling and gelling time of human HbSS erythrocytes were investigated. The study showed that the various metabolites inhibited sickling in vitro and increased gelling time of HbSS blood samples. Moreover, a significant increase in the number of ...

  17. Extraction, Characterization, Stability and Biological Activity of Flavonoids Isolated from Chamomile Flowers

    OpenAIRE

    Srivastava, Janmejai K; Gupta, Sanjay

    2009-01-01

    Dried flowers of Chamomile (Matricaria chamomilla) are largely used for their medicinal properties. In the present study, we examined the pharmacological properties of aqueous and methanolic fraction isolated from two varieties of German chamomile. HPLC-MS analysis of chamomile extract confirmed apigenin-7-O-glucoside as the major constituent of chamomile; some minor glycoside components were observed along with essential oils. These glucosides are highly stable in solution at different tempe...

  18. Water-soluble egg membrane enhances the immunoactivating properties of an Aloe vera-based extract of Nerium oleander leaves

    Directory of Open Access Journals (Sweden)

    Benson KF

    2016-11-01

    Full Text Available Kathleen F Benson,1 Robert A Newman,2,3 Gitte S Jensen1 1NIS Labs, Klamath Falls, OR, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center, Houston, 3Nerium Biotechnology Inc, San Antonio, TX, USA Objective: To evaluate a blend of two natural ingredients on immune parameters relevant for their current topical use and potential support of microcirculation in skin tissue. Materials and methods: A blend (BL of Aloe vera-based Nerium oleander extract (NAE-8i, oleandrin-free and hydrolyzed water-soluble egg membrane (WSEM was applied to human whole-blood cultures for 24 hours, with each separate ingredient serving as a control. Immune-cell subsets were analyzed for expression levels of the activation markers CD69 and CD25. Culture supernatants were analyzed for cytokines, chemokines, and immunoregulating peptides. Results: BL increased CD69 expression on lymphocytes, monocytes, and CD3–CD56+ natural killer cells, and CD25 expression on natural killer cells. The number of CD69+CD25+ lymphocytes increased in cultures treated with BL and the separate ingredients. BL triggered production of multiple cytokines and chemokines, where CC chemokines MIP1α and MIP3α, as well as cytokines involved in wound healing – Groα, Groβ, ENA78, and fractalkine – reached levels manyfold above treatment with either NAE-8i or WSEM alone. Conclusion: Data on BL showed that WSEM strongly enhanced NAE-8i’s effects on immunoactivation in vitro. This has potential relevance for support of immunity in skin tissue, including antibacterial and antiviral defense mechanisms, wrinkle reduction, and wound care. Keywords: chemokines, cytokines, leukocyte activation

  19. Antidiabetic and Free Radicals Scavenging Potential of Euphorbia hirta Flower Extract.

    Science.gov (United States)

    Kumar, S; Malhotra, R; Kumar, D

    2010-07-01

    The present study was carried out to evaluate antidiabetic and in vitro free radicals scavenging effects of flower extract of Euphorbia hirta. The ethanolic and petroleum ether extracts (250 and 500 mg/kg) were orally tested for 21 days in alloxan induced diabetic mice and blood glucose level was measured with glucometer. Administration of extract resulted in significant reduction in serum cholesterol, triglycerides, creatinine, urea, alkaline phosphatase levels but high density lipoprotein levels and total proteins were found to be increased after treatments. Free radicals scavenging effect of ethanolic extract was also evaluated by various antioxidant assays, including 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging activity, superoxide anion radical scavenging, nitric oxide scavenging, and reducing power assay. It was compared with standard antioxidants compounds such as butylated hydroxyl anisole and ascorbic acid. All the extracts showed antioxidant activity in all the tested methods.

  20. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats.

    Science.gov (United States)

    Nayak, B S; Pinto Pereira, Lexley M

    2006-12-21

    Catharanthus roseus L (C. roseus) has been used to treat a wide assortment of diseases including diabetes. The objective of our study was to evaluate the antimicrobial and wound healing activity of the flower extract of Catharanthus in rats. Wound healing activity was determined in rats, after administration (100 mg kg-1 day-1) of the ethanol extract of C. roseus flower, using excision, incision and dead space wounds models. The animals were divided into two groups of 6 each in all the models. In the excision model, group 1 animals were topically treated with carboxymethyl cellulose as placebo control and group 2 received topical application of the ethanol extract of C. roseus at a dose of 100 mg/kg body weight/day. In an incision and dead space model group 1 animals were given normal saline and group 2 received the extract orally at a dose of 100 mg kg-1 day-1. Healing was assessed by the rate of wound contraction, period of epithelization, tensile strength (skin breaking strength), granulation tissue weight, and hydoxyproline content. Antimicrobial activity of the flower extract against four microorganisms was also assessed The extract of C. roseus significantly increased the wound breaking strength in the incision wound model compared with controls (P comparison to control wounds (P Wet and dry granulation tissue weights, and hydroxyproline content in a dead space wound model increased significantly (p < 0.05). Pseudomonas aeruginosa and Staphylococcus aureus demonstrated sensitivity to C. roseus Increased wound contraction and tensile strength, augmented hydroxyproline content along with antimicrobial activity support the use of C. roseus in the topical management of wound healing.

  1. Effects of ethanolic extract of Jasminum grandiflorum Linn. flowers on wound healing in diabetic Wistar albino rats

    OpenAIRE

    Hirapara, Hiren; Ghori, Vishal; Anovadiya, Ashish; Baxi, Seema; Tripathi, Chandrabhanu

    2017-01-01

    Objective: To evaluate wound healing activity of ethanolic extract of Jasminum grandiflorum Linn. (J. grandiflorum) flowers in diabetic rats. Materials and Methods: Streptozotocin-induced diabetic Wistar albino rats were divided into six groups (n=6).Three groups – diabetic control, positive control (that received Glibenclamide) and treatment (that received J. grandiflorum Linn. Flower extract) were operated for excision wounds (EW). These groups were evaluated for wound contraction and re-ep...

  2. Antimicrobial Activity of Plant Extracts from Aloe Vera, Citrus Hystrix, Sabah Snake Grass and Zingiber Officinale against Pyricularia Oryzae that causes Rice Blast Disease in Paddy Plants

    Science.gov (United States)

    Uda, M. N. A.; Harzana Shaari, N.; Shamiera. Said, N.; Hulwani Ibrahim, Nur; Akhir, Maisara A. M.; Khairul Rabani Hashim, Mohd; Salimi, M. N.; Nuradibah, M. A.; Hashim, Uda; Gopinath, Subash C. B.

    2018-03-01

    Rice blast disease, caused by the fungus known as Pyricularia oryzae, has become an important and serious disease of rice worldwide. Around 50% of production may be lost in a field moderately affected by infection and each year the fungus destroys rice, which is enough to feed an estimated 60 million people. Therefore, use of herbal plants offer an alternative for the management of plant diseases. Herbal plant like Aloe vera, Citrus hystrix, Sabah snake grass and Zingiber officinale extracts can be used for controlling disease of rice blast. In this study, these four herbal plants were used for evaluating antimicrobial activity against rice plant fungus Pyricularia oryzae, which causes rice blast disease.

  3. Antibacterial activity of kecombrang flower extract (Nicolaia speciosa) microencapsulation with food additive materials formulation

    Science.gov (United States)

    Naufalin, R.; Rukmini, H. S.

    2018-01-01

    Kecombrang flower (Nicolaia speciosa) contains bioactive components of alkaloids, flavonoids, polyphenols, steroids, saponins, and essential oils as potential antimicrobials. The use of antibacterials in the form of essential oils has constraints; therefore microencapsulation needs to be done to prevent damage to the bioactive components. Microencapsulation can prevent degradation due to radiation or oxygen, easy-mix with foodstuffs and also slow the occurrence of evaporation. This study aimed to determine the effect of types of kecombrang extract, the concentration of microcapsules in food additives (NaCl and sucrose), and concentration of flower extract in the microcapsules. This study used Randomized Block Design (RBD) with 18 treatment combinations and two replications. Factors studied were types of kecombrang flower extract of (semi polar and polar extract), Food Additive types (sucrose and NaCl), the concentration of microcapsules in food additive (0%; 15%; 30% w /v). The results showed that polar and non-polar extract microcapsules produced antibacterial activity of 7.178 mm and 7.145 respectively of Bacillus cereus bacteria, while Escherichia coli was 7.272 mm and 7.289 mm respectively. A 30 percent microcapsule concentration provides antibacterial activity with inhibiting zone of 7, 818 mm for B. cereus and 8,045 for E.coli. Food Additive of sucrose concentrations showed that microcapsules produced tend to be more effective in inhibiting the growth of E.coli and B. cereus bacteria than that of NaCl, with each inhibition zone of 7.499 mm and 7.357 mm

  4. Aloe vera

    OpenAIRE

    Borges, Paula Marques; UFBA

    2014-01-01

    El Aloe Vera posee importantes propiedades curativas, entre las cuales podemos nombrar su capacidad como regenerador celular, su poder como desintoxicante, rehidratante y cicatrizante, su capacidad analgésica y antiinflamatoria, su poder antiséptico, antibiótico, fungicida y antivírico en algunos casos y por último, su capacidad como inmunomodulador, mejorando los niveles de anticuerpos en el organismo con su ingesta Objetivo: Determinar el grado de información sobre las propie...

  5. Extracts of Coreopsis tinctoria Nutt. Flower Exhibit Antidiabetic Effects via the Inhibition of α-Glucosidase Activity

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    Wujie Cai

    2016-01-01

    Full Text Available The aim of this study was to assay the effects of Coreopsis tinctoria Nutt. flower extracts on hyperglycemia of diet-induced obese mice and the underlying mechanisms. Coreopsis tinctoria flower was extracted with ethanol and water, respectively. The total phenol, flavonoid levels, and the constituents of the extracts were measured. For the animal experiments, C57BL/6 mice were fed with a chow diet, high-fat diet, or high-fat diet mixed with 0.4% (w/w water and ethanol extracts of Coreopsis tinctoria flower for 8 weeks. The inhibitory effects of the extracts on α-glucosidase activity and the antioxidant properties were assayed in vitro. We found that the extracts blocked the increase of fasting blood glucose, serum triglyceride (TG, insulin, leptin, and liver lipid levels and prevented the development of glucose tolerance impairment and insulin resistance in the C57BL/6 mice induced by a high-fat diet. The extracts inhibited α-glycosidase activity and increased oxidant activity in vitro. In conclusion, Coreopsis tinctoria flower extracts may ameliorate high-fat diet-induced hyperglycemia and insulin resistance. The underling mechanism may be via the inhibition of α-glucosidase activity. Our data indicate that Coreopsis tinctoria flower could be used as a beverage supplement and a potential source of drugs for treatment of diabetics.

  6. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (NAE-8®

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    Benson KF

    2015-05-01

    Full Text Available Kathleen F Benson,1 Robert A Newman,2,3 Gitte S Jensen1 1NIS Labs, Klamath Falls, Oregon, USA; 2University of Texas MD Anderson Cancer Center, Houston, TX, USA; 3Nerium Biotechnology, Inc, San Antonio, TX, USA Objective: The goal for this study was to evaluate the effects of an Aloe vera-based Nerium oleander extract (NAE-8®, compared to an extract of A. vera gel alone (ALOE, and to an aqueous extract of N. oleander (AQ-NOE in bioassays pertaining to dermatologic potential with respect to antioxidant protection, anti-inflammatory effects, and cytokine profiles in vitro. Methods: Cellular antioxidant protection was evaluated in three separate bioassays: The cellular antioxidant protection of erythrocytes (CAP-e assay, protection of cellular viability and prevention of apoptosis, and protection of intracellular reduced glutathione levels, where the last two assays were performed using human primary dermal fibroblasts. Reduction of intracellular formation of reactive oxygen species (ROS was tested using polymorphonuclear cells in the absence and presence of oxidative stress. Changes to cytokine and chemokine profiles when whole blood cells and human primary dermal fibroblasts were exposed to test products were determined using a 40-plex Luminex array as a method for exploring the potential cross-talk between circulating and skin-resident cells. Results: The NAE-8® provided significantly better antioxidant protection in the CAP-e bioassay than AQ-NOE. NAE-8® and AQ-NOE both protected cellular viability and intracellular reduced glutathione, and reduced the ROS formation significantly when compared to control cells, both under inflamed and neutral culture conditions. ALOE showed minimal effect in these bioassays. In contrast to the NAE-8®, the AQ-NOE showed induction of inflammation in the whole blood cultures, as evidenced by the high induction of CD69 expression and secretion of a number of inflammatory cytokines. The treatment of dermal

  7. Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)?A Review

    OpenAIRE

    Cristiano, Giuseppe; Murillo-Amador, Bernardo; De Lucia, Barbara

    2016-01-01

    Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important fac...

  8. Extraction, Characterization, Stability and Biological Activity of Flavonoids Isolated from Chamomile Flowers

    Science.gov (United States)

    Srivastava, Janmejai K; Gupta, Sanjay

    2009-01-01

    Dried flowers of Chamomile (Matricaria chamomilla) are largely used for their medicinal properties. In the present study, we examined the pharmacological properties of aqueous and methanolic fraction isolated from two varieties of German chamomile. HPLC-MS analysis of chamomile extract confirmed apigenin-7-O-glucoside as the major constituent of chamomile; some minor glycoside components were observed along with essential oils. These glucosides are highly stable in solution at different temperature range and their degradation occurs after long-term storage and extraction conditions at different pH and solvent. Methanolic fraction isolated from chamomile flowers demonstrated higher biologic response in inhibiting cell growth and causing induction of apoptosis in various human cancer cell lines compared to aqueous chamomile fraction. Apigenin glucosides inhibited cancer cell growth through deconjugation of glycosides that occurs in the cellular compartment to produce aglycone, apigenin. Taken together, the pharmacological profile of chamomile extract was dependent upon extraction process, storage conditions which affected the biological activity. PMID:20098626

  9. Antioxidant activity of flower, stem and leaf extracts of Ferula gummosa Boiss

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    Mohammad Nabavi, Seyed

    2010-09-01

    Full Text Available The Antioxidant and antihemolytic activities of hydroalcoholic extracts of the flowers, stems and leaves of the Ferula gummosa Boiss were investigated employing different in vitro assay systems. Leaf extract showed better activity in DPPH radical scavenging. In addition it showed better activity in nitric oxide and H2O2 scavenging and Fe2+ chelating activity than the other parts. The extracts exhibited good antioxidant activity in linoleic acid system test but were not comparable with vitamin C (p2O2 induced hemolysis. Among the extracts, the flowers had higher phenolic and flavonoid contents. This plant is very promising for further biochemical experiments.La actividad antihemolítica y antioxidante de extractos hidroalcohólicos de flores, tallos y hojas de Ferula gummosa Boiss fueron investigados empleando diferentes ensayos in vitro. Los extractos de hojas mostraron una mejor actividad captadora de radicales libres. Además, mostraron una mejor actividad captadora de óxido nítrico y H2O2 y actividad quelatante de Fe2+ que las otras partes. Los extractos exhibieron una buena actividad antioxidante en el ensayo con ácido linoleico pero no comparable con la vitamina C (pF. gummosa mostraron una mejor actividad antihemolítica contra la hemolisis inducida con H2O2. Entre los extractos, las flores tienen los más altos contenidos de fenoles y flavonoides. Esta planta es muy prometedora para futuros experimentos bioquímicos.

  10. The effect of ethanol extract of ylang-ylang flower (Cananga odorata on vascular and kidney histology in menopausal mice

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    Juliastuti Juliastuti

    2017-10-01

    Full Text Available This study aimed to evaluate the histopathological changes in the aorta and kidneys of mice age of menopause by the ethanol extract of ylang-ylang (Cananga odorata flowers. Thirty female age of menopause mice was divided into three groups (n = 10 each group, consisting of a group of mice the age of menopause without any treatment, mice age of menopause treated with different dosages of ylang-ylang flower extract (0.1 mg/day and 0.2 mg/day. Histopathological analysis was performed with hematoxylin-eosin staining. The number of endothelial cells and the thickness of the intima in the group treated with high dose of the extract were significantly higher than the lowest dose of the extract or the control group (p < 0.05. The diameter of the Bowman's capsule and the renal glomerulus was significantly higher in the group treated with ylang-ylang flower extract compared with the control group (p < 0.05. Concluded that at the doses administered, ylang flower extract triggers the repair of endothelial cells, but increase the thickness of the intima and hyperplasia of the kidney in mice age of menopause. Thus, ylang flower extract and renal vascular remodeling triggered by the age of menopause.

  11. Comparative Evaluation of Fresh Aloe barbadensis Plant Extract and Mineral Trioxide Aggregate as Pulpotomy Agents in Primary Molars: A 12-month Follow-up Study

    Science.gov (United States)

    Kalra, Mehak; Garg, Nishita; Rallan, Mandeep; Pathivada, Lumbini; Yeluri, Ramakrishna

    2017-01-01

    Background: The purpose of this study was to compare the clinical and radiographic outcomes of fresh Aloe vera barbadensis plant extract and mineral trioxide aggregate (MTA) as pulpotomy agents in primary molar teeth. Materials and Methods: Pulpotomy procedure was performed in sixty primary molar teeth which were randomly allocated to two groups, i.e., Aloe vera pulpotomy (Group A) and MTA pulpotomy (Group B). All the pulpotomized teeth were evaluated clinically and radiographically at 1, 3, 6, 9, and 12 months of time interval using predetermined criteria. Results: The success rates between Groups A and B at the end of the 1st month were 24.1% and 96.4%, at the end of 3rd month were 57.1% and 100%, at the end of 6th month were 75% and 100%, at the end of 9th month were 66.6% and 100%, and at the end of 12 months were 100% and 100% respectively. The overall success rates at the end of 12-month follow-up period were 6.9% and 71.4%, respectively, after taking dropout patients into consideration, and the difference was statistically significant (P < 0.001). Conclusions: MTA pulpotomy was found to be superior when compared to fresh A. barbadensis plant extract pulpotomy in primary molars. PMID:28566860

  12. Antidiarrhoeal property of the hydroethanolic extract of the flowering tops of Anthocephalus cadamba

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    M. Ashraful Alam

    Full Text Available The antidiarrhoeal property of the hydroethanolic extract of the flowering tops of Anthocephalus cadamba was assessed on experimental animals. The dry hydroethanolic extract (250-500 mg/kg body mass, p.o. exhibited a dose-dependent decrease in the total number of faecal droppings in castor oil-induced diarrhoea in mice. The extract also produced a significant (p < 0.01 and dose-dependent reduction in intestinal fluids accumulation and in the gastrointestinal transit from 64.59 % and 71.19% at doses of 250 and 500 mg/kg. The reduction rates were 37.85% and 74.91%, respectively, with the control and standard drug group.

  13. In vitro antioxidant properties of Cucurbita pepo L. male and female flowers extracts.

    Science.gov (United States)

    Tarhan, Leman; Kayali, Hulya Ayar; Urek, Raziye Ozturk

    2007-06-01

    Total antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (DPPH.), hydroxyl (HO.), scavenging activities, and total phenolic values were determined in extracts of Cucurbita pepo L. female and male flowers. Powdered C. pepo L. samples were extracted in aqueous ethyl acetate (EA: W1, 17:3), ethanol (E), and water (W) by agitating in magnetic stirrer for 80 degrees C, 15 min and also by in aqueous ethyl acetate (EA: W2, 17:3) at 25 degrees C, 15 min. DPPH., HO. scavenging capacities and total phenolic values of C. pepo L. female and male were higher in EA:W2 than in other extracts. In addition, all determined antioxidant capacities of female were significantly higher than male.

  14. Aloe vera on Lanzarote

    OpenAIRE

    Walker, Colin

    2016-01-01

    Aloe vera is the basis of a multibillion dollar cosmetics and toiletries industry worldwide. It is grown commercially in many parts of the world and here its cultivation is described on an aloe farm in the north of Lanzarote, the Canary Islands. The products from such monoculture are sold throughout the island in Aloe vera shops run principally by the company aloe Lanzarote.

  15. Larvicidal activity of Nerium oleander L. (Apocynaceae flower extracts against Culex quinquefasciatus Say (Diptera: Culicidae

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    R. Raveen

    2014-03-01

    Full Text Available Culex quinquefasciatus is the vector responsible for serious disease filariasis among human beings. Plant derived products have received increased attention from scientists as they serve as a rich source for novel natural substances possessing insecticidal properties which are safe to human and ecosystem. During the last decade, various studies on natural plant products against vector mosquito indicate them as possible alternatives to chemical and synthetic insecticides for mosquito control. In the present study, the crude hexane and aqueous extract of Nerium oleander flowers were reported for larvicidal activity against the filarial vector, Culex quinquefasciatus. Mortality was observed for 24 and 48 hours. Hexane flower extract exhibited highest larvicidal activity with a LC50 value of 102.54 ppm and 61.11ppm after 24 and 48 hours respectively. Further investigations are needed to elucidate this activity against a wide range of all stages of mosquito species and also the active ingredient(s of the extract responsible for larvicidal activity should be identified.

  16. Antioxidant, anticancer and anticholinesterase activities of flower, fruit and seed extracts of Hypericum amblysepalum HOCHST.

    Science.gov (United States)

    Keskin, Cumali

    2015-01-01

    Cancer is an unnatural type of tissue growth in which the cells exhibit unrestrained division, leading to a progressive increase in the number of dividing cells. It is now the second largest cause of death in the world. The present study concerned antioxidant, anticancer and anticholinesterase activities and protocatechuic, catechin, caffeic acid, syringic acid, p-coumaric acid and o-coumaric concentrations in methanol extracts of flowers, fruits and seeds of Hypericum amblysepalum. Antioxidant properties including free radical scavenging activity and reducing power, and amounts of total phenolic compounds were evaluated using different tests. Protocatechuic, catechin, caffeic acid, syringic acid, p-coumaric acid and o-coumaric concentrations in extracts were determined by HPLC. Cytotoxic effects were determined using the MTT test with human cervix cancer (HeLa) and rat kidney epithelium cell (NRK-52E) lines. Acetyl and butyrylcholinesterase inhibitory activities were measured by by Ellman method. Total phenolic content of H. amblysepalum seeds was found to be higher than in fruit and flower extracts. DPPH free radical scavenging activity of the obtained extracts gave satisfactory results versus butylated hydroxyanisole and butylated hydroxytoluene as controls. Reducing power activity was linearly proportional to the studied concentration range: 10-500 μg/ mL LC50 values for H. amblysepalum seeds were 11.7 and 2.86 respectively for HeLa and NRK-52E cell lines. Butyryl-cholinesterase inhibitory activity was 76.9±0.41 for seed extract and higher than with other extracts. The present results suggested that H. amblysepalum could be a potential candidate anti-cancer drug for the treatment of human cervical cancer, and good source of natural antioxidants.

  17. Dyeing Performance of Aqueous Extract and Flavanone Glycosides from the Flowers of Butea monosperma (Lam. Kuntze

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    Ruchi Badoni Semwal

    2014-01-01

    Full Text Available Aqueous extract and two flavanone glycosides named 5,7-dihydroxy-4’-methoxy flavanone-5-β-D-glucopyranosyl-(1→2-β-D-glucopyranoside (1 and 5,5’-dihydroxy-4’,7-dimethoxyflavanone-5,5’-di-O-β-D-glucopyranoside (2 obtained from the flowers of Butea monosperma were studied for evaluate their dyeing properties on cotton fibers. The stem bark of Myrica esculenta was used as natural mordant whereas SnCl 2 and FeCl 3 as synthetic mordants. The combination of dye with mordants showed interesting shades with excellent washing and light fastness properties.

  18. Preliminary Studies on Antimicrobial Activity of Extracts from Aloe Vera Leaf, Citrus Hystrix Leaf, Zingiber Officinale and Sabah Snake Grass Against Bacillus Subtilis

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    Uda M.N.A.

    2018-01-01

    Full Text Available Herbal plants have several potential antimicrobial activities either as antifungal or antibacterial to fight against the disease and pathogen that attack the plants. The extractions of the Aloe vera leaf, Citrus hystrix leaf, Zingiber officinale rhizome and Sabah snake grass were selected in this study to fight against Bacillus subtilis. B. subtilis is a Gram-positive bacterium, rodshaped and catalase-positive that lives on decayed organic material. It is known as Gram-positive bacteria because of its thick peptidoglycan and would appear purple when subjected to Gram test. This species is commonly found in the upper layers of the soil, in meat or vegetables, in pastry, cooked meat, in bread or poultry products. The extracts of Sabah Snake Grass found to be most effective than A.vera leaf, Z. officinale, and C. hystrix against the B. subtilis.

  19. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Science.gov (United States)

    Afiune, Luana Alves Freitas; Leal-Silva, Thaís; Sinzato, Yuri Karen; Moraes-Souza, Rafaianne Queiroz; Soares, Thaigra Sousa; Campos, Kleber Eduardo; Fujiwara, Ricardo Toshio; Herrera, Emilio; Damasceno, Débora Cristina; Volpato, Gustavo Tadeu

    2017-01-01

    The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes. Diabetes was induced by streptozotocin (STZ, 40 mg/kg) in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group): non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed. The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI) and coronary artery risk index (CRI), and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group. Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  20. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Directory of Open Access Journals (Sweden)

    Luana Alves Freitas Afiune

    Full Text Available The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes.Diabetes was induced by streptozotocin (STZ, 40 mg/kg in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group: non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed.The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI and coronary artery risk index (CRI, and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group.Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  1. Chemical characterization and insecticidal activity of Calotropis gigantea L. flower extract against Tribolium castaneum (Herbst

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    Muhammad Rowshanul Habib

    2016-12-01

    Full Text Available Objective: To test the insecticidal activity of ethyl acetate extract of Calotropis gigantea L. flower (designated as EECF against stored grain pest Tribolium castaneum (Herbst of different larval and adult stages. Methods: Residual film method was used here to study the toxicity of EECF against Tribolium castaneum and gas chromatography-mass spectrometer analysis was also performed to characterize the chemicals of EECF. Results: In residual film bioassay, EECF showed lowest LD50 (0.134 mg/cm2 against 1st instar larvae of Tribolium castaneum and this finding ultimately revealed that the insect of initial stage was more susceptible than other stages. From the results of this study, it was found that with the increasing of age, Tribolium castaneum showed some extent of resistance against the toxicity of EECF. Moreover, chemical profiles of EECF identified by gas chromatography-mass spectrometer analysis were also found to consistent with its insecticidal activity. Conclusions: So, the overall results suggested that extracts of Calotropis gigantea L. flower have potential insecticidal effect which might be used in pest control.

  2. Effect of Microwave-Assisted Extraction on the Phenolic Compounds and Antioxidant Capacity of Blackthorn Flowers

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    Vanja Lovrić

    2017-01-01

    Full Text Available This research was undertaken to investigate the influence of extraction parameters during microwave-assisted extraction on total phenolic content, total flavonoids, total hydroxycinnamic acids and total flavonols of blackthorn flowers as well as to evaluate the antioxidant capacity by two different methods (2,2-diphenyl-1 picrylhydrazyl free radical scavenging capacity and ferric reducing antioxidant power assays. The investigated extraction parameters were: solvent type and volume fraction of alcohol in solvent (50 and 70 % aqueous solutions of ethanol and methanol, extraction time (5, 15 and 25 min and extraction temperature (40, 50 and 60 °C controlled by microwave power of 100, 200 and 300 W. Multivariate analysis of variance (MANOVA was used to evaluate the differences at a 95 % confidence level (p≤0.05. The obtained results show that aqueous solution of ethanol was more appropriate solvent for extraction of phenolic compounds (total flavonoids, total hydroxycinnamic acids and total flavonols than aqueous solution of methanol. The amount of phenolic compounds was higher in 70 % aqueous solution of ethanol or methanol, while higher antioxidant capacity was observed in 50 % aqueous solution of methanol. Higher temperature of extraction improved the amount of phenolic compounds and also antioxidant capacity determined by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging capacity assay. Extensive duration of extraction (15- to 25-minute interval has a significant effect only on the increase of total phenolic content, while specific phenolic compound content and antioxidant capacity were the highest when microwave extraction time of 5 min was applied.

  3. Green synthesis and characterization of silver nanoparticles using Alcea rosea flower extract as a new generation of antimicrobials

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    Ebrahiminezhad Alireza

    2017-01-01

    Full Text Available Green synthesis of silver nanoparticles (AgNPs was developed by treating Ag+ with Alcea rosea flower extract. AgNO3 concentration, flower extract quantity, and reaction temperature were found to be significant factors in the bioreduction reaction. Synthesized AgNPs were almost spherical in shape with an average diameter of 7.2 nm. Fourier transform infrared spectroscopy (FTIR analysis revealed that oxygen-bearing functional groups in the A. rosea flower extract are responsible for reduction of Ag+. The minimum inhibitory concentration (MIC of AgNPs against a Gram-positive (Staphylococcus aureus and Gram-negative (Escherichia coli bacteria was determined to be 37.5 μg/ml.

  4. Antidepressant-like effects of methanol extract of Hibiscus tiliaceus flowers in mice

    Science.gov (United States)

    2012-01-01

    Background Hibiscus tiliaceus L. (Malvaceae) is used in postpartum disorders. Our purpose was to examine the antidepressant, anxiolytic and sedative actions of the methanol extract of H. tiliaceus flowers using animal models. Methods Adult male Swiss albino mice were treated with saline, standard drugs or methanol extract of H. tiliaceus and then subjected to behavioral tests. The forced swimming and tail suspension tests were used as predictive animal models of antidepressant activity, where the time of immobility was considered. The animals were submitted to the elevated plus-maze and ketamine-induced sleeping time to assess anxiolytic and sedative activities, respectively. Results Methanol extract of H. tiliaceus significantly decreased the duration of immobility in both animal models of antidepressant activity, forced swimming and tail suspension tests. This extract did not potentiate the effect of ketamine-induced hypnosis, as determined by the time to onset and duration of sleeping time. Conclusion Our results indicate an antidepressant-like profile of action for the extract of Hibiscus tiliaceus without sedative side effect. PMID:22494845

  5. The antimosquito properties of extracts from flowering plants in South Africa.

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    Chalannavar, R K; Hurinanthan, V; Singh, A; Venugopala, K N; Gleiser, R M; Baijnath, H; Odhav, B

    2013-12-01

    Extracts of selected flowering plants, which are considered eco-friendly, are used for the treatment of numerous ailments and vector control worldwide. This has resulted in approximately 25 per cent of currently used drugs being derived from herbal sources. The aqueous and methanolic extracts of twelve plant species, Psidium guajava (pink fruit), Psidium guajava (white fruit), Psidium cattleianum var. cattleianum, Psidium guineense and Psidium X durbanensis, Achyranthes aspera, Alternanthera sessilis, Guilleminea densa, Capparis tomentosa, Leonotis leonurus, Dichrostachys cinerea and Carpobrotus dimidiatus, were tested for insecticidal activity, including larvicidal, adulticidal and repellent activities against the adult female mosquito, Anopheles arabiensis. The extracts of P. guajava (white fruit), C. tomentosa, L. leonurus,D. cinerea, and C. dimidiatus exerted a pronounced inhibitory effect on adult insects, while those of P. guajava (pink fruit), P. X durbanensis, P. cattleianum var. cattleianum, P. guineense, A. aspera, A. sessilis, and G. densa were ineffective and failed to satisfy the criteria set by the World Health Organization. In the tests for repellency against An. arabiensis, all the tested aqueous and methanolic plant extracts except those of A. sessilis repelled 80-100% of mosquitoes. The most effective mosquito repellents were the methanol and aqueous extracts of P. guajava (pink fruit), P. X durbanensis, P. cattleianum var. cattleianum, P. guineense, G. densa,L. leonurus and D. cinerea, which are potential sources of cost effective mosquito repellents to be utilized in malarial endemic areas.

  6. Antidepressant-like effects of methanol extract of Hibiscus tiliaceus flowers in mice

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    Vanzella Cláudia

    2012-04-01

    Full Text Available Abstract Background Hibiscus tiliaceus L. (Malvaceae is used in postpartum disorders. Our purpose was to examine the antidepressant, anxiolytic and sedative actions of the methanol extract of H. tiliaceus flowers using animal models. Methods Adult male Swiss albino mice were treated with saline, standard drugs or methanol extract of H. tiliaceus and then subjected to behavioral tests. The forced swimming and tail suspension tests were used as predictive animal models of antidepressant activity, where the time of immobility was considered. The animals were submitted to the elevated plus-maze and ketamine-induced sleeping time to assess anxiolytic and sedative activities, respectively. Results Methanol extract of H. tiliaceus significantly decreased the duration of immobility in both animal models of antidepressant activity, forced swimming and tail suspension tests. This extract did not potentiate the effect of ketamine-induced hypnosis, as determined by the time to onset and duration of sleeping time. Conclusion Our results indicate an antidepressant-like profile of action for the extract of Hibiscus tiliaceus without sedative side effect.

  7. In vitro antioxidant activity of different cultivars of banana flower (Musa paradicicus L.) extracts available in India.

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    China, Ratna; Dutta, Sanjukta; Sen, Sauradip; Chakrabarti, Rajarshi; Bhowmik, Debajit; Ghosh, Santinath; Dhar, Pubali

    2011-01-01

    Six different cultivars of banana flowers (Musa paradicicus) (Kathali, Bichi, Shingapuri, Kacha, Champa, and Kalabou) were analyzed for the content of polyphenol expressed as gallic acid equivalent and flavonoid expressed as quercetein equivalent, and the in vitro total antioxidative activities of the flower extracts were compared with standard and expressed as trolox equivalent. The reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS•(+)) scavenging activities, inhibition of lipid peroxidation in a linoleic acid emulsion system, and liposome peroxidation system were measured and compared with respective standard antioxidants. Iron-mediated Fenton reaction was carried out to evaluate the protective effect of the extract of banana flower (Kacha cultivar) against H(2)O(2)-induced DNA damage. The Kacha variety contains the maximum amount of polyphenol (11.94 ± 0.03 mg of gallic acid equivalent/g of dry weight) and flavonoid (0.174 ± 0.001 g of quercetin equivalent/g of polyphenol). It also has the highest total antioxidant capacity, DPPH radical scavenging activity, and ABTS•(+) radical scavenging activity with a least EC(50) value of 0.051 mg/mL. Hepatic cell damage in iron-mediated Fenton reaction caused by free radicals is reduced by the banana flower extract. On the basis of the results obtained, the banana flowers are found to be a potential source of natural antioxidants. This is the first report on the antioxidant properties of the extracts from banana flowers. The study suggests that the flowers of M. paradicicus that are found in India and consumed as vegetable can provide valuable functional ingredients that help in the prevention of oxidative stress. © 2011 Institute of Food Technologists®

  8. PROPAGATION TECHNIQUES AND AGRONOMIC REQUIREMENTS FOR THE CULTIVATION OF BARBADOS ALOE (ALOE VERA (L. BURM. F. - A REVIEW.

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    Barbara De Lucia

    2016-09-01

    Full Text Available Barbados aloe (Aloe vera (L. Burm. f. has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe and America.In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

  9. Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

    Science.gov (United States)

    Cristiano, Giuseppe; Murillo-Amador, Bernardo; De Lucia, Barbara

    2016-01-01

    Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems. PMID:27721816

  10. In Vitro Antioxidant Effects of Aloe barbadensis Miller Extracts and the Potential Role of These Extracts as Antidiabetic and Antilipidemic Agents on Streptozotocin-Induced Type 2 Diabetic Model Rats

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    Md. Ibrahim Khalil

    2012-11-01

    Full Text Available In this study, the total phenolic and flavonoid contents, the 2,2-diphenyl-1-picryl hydrazyl (DPPH radical scavenging ability and the ferric reducing power (FRAP of Aloe vera were measured to determine the antioxidant activity of this species. The in vivo antidiabetic effects of the plant were also investigated using streptozotocin-induced type 2 diabetic model rats that were divided into five groups based on the treatment received: (1 water (WC; (2 glibenclamide; (3 concentrated gel extract (Gel-C; (4 ethanol (80% gel extract (Gel-Et; and (5 ethanol (80% skin extract of Aloe vera (Skin-Et. Skin-Et, which contained the highest level of total phenolics (62.37 ± 1.34 mggallic acid/kg and flavonoids (20.83 ± 0.77 mg/kg, exhibited the highest scavenging activity (85.01 ± 0.52% and the greatest reducing power (185.98 ± 0.41 µM, indicating that the skin contained the highest level of antioxidants. The oral consumption of Gel-Et for 4 weeks a caused significant reduction in the fasting serum glucose levels of the rats. The rats in the Gel-C-, Gel-Et- and Skin-Et-treated groups experienced a reduction in their total cholesterol levels by 11%, 17% and 25%, respectively and a reduction in their LDL cholesterol levels by 45%, 3% and 69%, respectively. The in vivo experimental antioxidant parameter MDA is strongly correlated with the in vitro antioxidant parameters of flavonoids and polyphenols, namely the DPPH and FRAP values (r = 0.94, 0.92, 0.93, 0.90, thus confirming the antioxidant potential of the Aloe vera extracts.

  11. Activation of AMPK by Buddleja officinalis Maxim. Flower Extract Contributes to Protecting Hepatocytes from Oxidative Stress.

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    Jung, Ji Yun; Lee, Chul Won; Park, Sang Mi; Jegal, Kyung Hwan; Kim, Jae Kwang; Park, Chung A; Cho, Il Je; Jung, Dae Hwa; An, Won G; Ku, Sae Kwang; Zhao, Rongjie; Kim, Sang Chan

    2017-01-01

    The Buddleja officinalis Maxim. flower is used in traditional Chinese and Korean medicine to treat inflammation, vascular diseases, headache, and stroke, as well as enhance liver function. This research investigated the effects of B. officinalis Maxim. flower extract (BFE) on hepatotoxicity. The cytoprotective effects and mechanism of BFE against severe mitochondrial dysfunction and H 2 O 2 production in hepatotoxicity induced by coadministration of arachidonic acid (AA) and iron were observed in the HepG2 cell line. In addition, we performed blood biochemical, histopathological, and histomorphometric analyses of mice with carbon tetrachloride- (CCl 4 -) induced acute liver damage. BFE inhibited the AA + iron-mediated hepatotoxicity of HepG2 cells. Moreover, it inhibited mitochondrial dysfunction, H 2 O 2 production, and glutathione depletion mediated by AA + iron in the same cells. Meanwhile, the cytoprotective effects of BFE against oxidative stress were associated with the activation of AMP-activated protein kinase (AMPK). In particular, based on the histopathological observations, BFE (30 and 100 mg/kg) showed clear hepatoprotective effects against CCl 4 -induced acute hepatic damage. Furthermore, it inhibited 4-hydroxynonenal and nitrotyrosine immunoreactivity in hepatocytes. These results provide evidence that BFE has beneficial hepatoprotective effects against hepatic damage via the activation of AMPK pathway. Accordingly, BFE may have therapeutic potential for diverse liver disorders.

  12. Activation of AMPK by Buddleja officinalis Maxim. Flower Extract Contributes to Protecting Hepatocytes from Oxidative Stress

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    Ji Yun Jung

    2017-01-01

    Full Text Available The Buddleja officinalis Maxim. flower is used in traditional Chinese and Korean medicine to treat inflammation, vascular diseases, headache, and stroke, as well as enhance liver function. This research investigated the effects of B. officinalis Maxim. flower extract (BFE on hepatotoxicity. The cytoprotective effects and mechanism of BFE against severe mitochondrial dysfunction and H2O2 production in hepatotoxicity induced by coadministration of arachidonic acid (AA and iron were observed in the HepG2 cell line. In addition, we performed blood biochemical, histopathological, and histomorphometric analyses of mice with carbon tetrachloride- (CCl4- induced acute liver damage. BFE inhibited the AA + iron-mediated hepatotoxicity of HepG2 cells. Moreover, it inhibited mitochondrial dysfunction, H2O2 production, and glutathione depletion mediated by AA + iron in the same cells. Meanwhile, the cytoprotective effects of BFE against oxidative stress were associated with the activation of AMP-activated protein kinase (AMPK. In particular, based on the histopathological observations, BFE (30 and 100 mg/kg showed clear hepatoprotective effects against CCl4-induced acute hepatic damage. Furthermore, it inhibited 4-hydroxynonenal and nitrotyrosine immunoreactivity in hepatocytes. These results provide evidence that BFE has beneficial hepatoprotective effects against hepatic damage via the activation of AMPK pathway. Accordingly, BFE may have therapeutic potential for diverse liver disorders.

  13. Influence of Cempaka Yellow Flower Extract Michelia Champaca L. on Lipid Profile on The Menopause Age Rate

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    Meliani Sukmadewi Harahap

    2017-11-01

    Full Text Available The number of women aged 50 years and over can be estimated to increase from 500 million at present to more than 1 billion in prediction during 2030. After entering menopause the hormone estrogen in the body of women is decreased drastically. Decreased estrogen hormone makes LDL cholesterol difficult to control high levels of LDL one of the factors causing coronary heart disease. Estrogen is a female hormone that has many functions one of which is an antioxidant which controls of levels of cholesterol. The Cempaka Yellow Flower Michelia champaca L or known as the Flower Jeumpa in Aceh Province one of the herbs which are useful as antioxidants antimicrobials anti-inflammatory antidiabetic and antifungal. The several components contain as flavonoids polyphenols phenols tannins anthocyanins glutathione and Vitamin C. The general objectives of this study were To prove the effect of giving of Cempaka Yellow Flower extract Michelia champaca L has lipid profile in mouse age of menopause. The design of this study is true experimental design with the randomized posttest-only control group design approach. Measurements of HDL LDL triglyceride and total cholesterol levels were performed only after the treatment of young flower extract was performed. The subjects of this study were female winstar rats age of menopause as many as 28 tail. The Cempaka Yellow Flower extract can lower LDL and cholesterol levels and can increase HDL levels. Yellow Cempaka able to decrease LDL and Cholesterol level and increase HDL level at optimum dose 300mgKgWBday .

  14. Supercritical carbon dioxide extraction of chamomile flowers: extraction efficiency, stability, and in-line inclusion of chamomile-carbon dioxide extract in beta-cyclodextrin.

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    Kaiser, C S; Römpp, H; Schmidt, P C

    2004-01-01

    The extraction of chamomile flowers using supercritical carbon dioxide was investigated with respect to extraction efficiency and compared with solvent extraction. The stability of matricine, a sensitive constituent of the essential oil of chamomile, in these extracts was studied during storage at different temperatures over 6 months. Matricine was stable at -30 degrees C. A slight decrease (80-90% recovery) occurred at +5 degrees C, whereas complete decomposition of matricine took place within 3-4 months at room temperature and at +30 degrees C, respectively. An in-line inclusion of chamomile constituents in beta-cyclodextrin (beta-CD) during the extraction process was assessed and inclusion rates between 40 and 95% were obtained depending on the amount of beta-CD and the type of chamomile constituent. No further stabilization of matricine in the carbon dioxide extract/beta-CD complexes was achieved. High residual water contents in the complexes even after freeze-drying were identified as accelerating the decomposition. In addition, the extractability of flavonoids, such as apigenin and apigenin-7-glucoside, was determined. Apigenin-7-glucoside, the more hydrophilic substance, was not extractable with pure carbon dioxide and showed a recovery of 11% using methanol modified carbon dioxide (18%, w/w) at 60 degrees C and 380 bar. Extraction conditions in the two-phase region of the binary mixture carbon dioxide-methanol (70 degrees C, 100 bar) led to a drastic change in fluid polarity and hence extractability increased to 92-95%.

  15. Optimization of Ultrasound-Assisted Extraction of Natural Antioxidants from the Flower of Jatropha integerrima by Response Surface Methodology.

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    Xu, Dong-Ping; Zhou, Yue; Zheng, Jie; Li, Sha; Li, An-Na; Li, Hua-Bin

    2015-12-24

    An ultrasound-assisted extraction (UAE) method was developed for the efficient extraction of natural antioxidants from the flowers of Jatropha integerrima. Four independent variables, including ethanol concentration, solvent/material ratio, ultrasound irradiation time and temperature were studied by single factor experiments. Then, the central composite rotatable design and response surface methodology were employed to investigate the effect of three key parameters (ethanol concentration, solvent/material ratio, and ultrasound irradiation time) on the antioxidant activities of the flower extracts. The optimal extraction conditions were an ethanol concentration of 59.6%, solvent/material ratio of 50:1, ultrasound irradiation time of 7 min, and ultrasound irradiation temperature of 40 °C. Under these conditions, the optimized experimental value was 1103.38 ± 16.11 µmol Trolox/g dry weight (DW), which was in accordance with the predicted value (1105.49 µmol Trolox/g DW). Furthermore, the antioxidant activities of flower extracts obtained by UAE were compared with those produced by the traditional maceration and Soxhlet extraction methods, and UAE resulted in higher antioxidant activities after a shorter time at a lower temperature. The results obtained are helpful for the full utilization of Jatropha integerrima, and also indicate that ultrasound-assisted extraction is an efficient method for the extraction of natural antioxidants from plant materials.

  16. Optimization of Ultrasound-Assisted Extraction of Natural Antioxidants from the Flower of Jatropha integerrima by Response Surface Methodology

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    Dong-Ping Xu

    2015-12-01

    Full Text Available An ultrasound-assisted extraction (UAE method was developed for the efficient extraction of natural antioxidants from the flowers of Jatropha integerrima. Four independent variables, including ethanol concentration, solvent/material ratio, ultrasound irradiation time and temperature were studied by single factor experiments. Then, the central composite rotatable design and response surface methodology were employed to investigate the effect of three key parameters (ethanol concentration, solvent/material ratio, and ultrasound irradiation time on the antioxidant activities of the flower extracts. The optimal extraction conditions were an ethanol concentration of 59.6%, solvent/material ratio of 50:1, ultrasound irradiation time of 7 min, and ultrasound irradiation temperature of 40 °C. Under these conditions, the optimized experimental value was 1103.38 ± 16.11 µmol Trolox/g dry weight (DW, which was in accordance with the predicted value (1105.49 µmol Trolox/g DW. Furthermore, the antioxidant activities of flower extracts obtained by UAE were compared with those produced by the traditional maceration and Soxhlet extraction methods, and UAE resulted in higher antioxidant activities after a shorter time at a lower temperature. The results obtained are helpful for the full utilization of Jatropha integerrima, and also indicate that ultrasound-assisted extraction is an efficient method for the extraction of natural antioxidants from plant materials.

  17. Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract

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    Renata Dobrucka

    2016-07-01

    Full Text Available Zinc oxide (ZnO has broad applications in various areas. Nanoparticle synthesis using plants is an alternative to conventional physical and chemical methods. It is known that the biological synthesis of nanoparticles is gaining importance due to its simplicity, eco-friendliness and extensive antimicrobial activity. Also, in this study we report the synthesis of ZnO nanoparticles using Trifolium pratense flower extract. The prepared ZnO nanoparticles have been characterized by UV–Vis absorption spectroscopy, X-ray diffraction (XRD, Fourier transform infrared spectroscopy (FT-IR, and scanning electron microscopy (SEM with Energy dispersive X-ray analysis (EDX. Besides, this study determines the antimicrobial efficacy of the synthesized ZnO nanoparticles against clinical and standard strains of S. aureus and P. aeruginosa and standard strain of E. coli.

  18. Polyphenolic Extracts of Edible Flowers Incorporated onto Atelocollagen Matrices and Their Effect on Cell Viability

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    Jorge López-García

    2013-10-01

    Full Text Available The phenolic extract of chives flowers (Allium schoenoprasum, Liliaceae, introduced Sage (Salvia pratensis, Lamiaceae, European elderberry (Sambucus nigra, Caprifoliaceae and common dandelion (Taraxacum officinale, Asteraceae were characterised by High Performance Liquid Chromatography and incorporated in different concentrations onto atelocollagen thin films. In order to assess the biological impact of these phenolic compounds on cell viability, human immortalised non-tumorigenic keratinocyte cell line was seeded on the thin films and cell proliferation was determined by using an MTT assay. In addition, their antimicrobial activity was estimated by using an agar diffusion test. Data indicated the concomitance between cell viability and concentration of polyphenols. These findings suggest that these phenolic-endowed atelocollagen films might be suitable for tissue engineering applications, on account of the combined activity of polyphenols and collagen.

  19. Aqueous extract from Brownea grandiceps flowers with effect on coagulation and fibrinolytic system.

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    Pereira, Betzabeth; Brazón, Josmary

    2015-02-03

    Brownea grandiceps flowers are used in Venezuelan folk medicine as anti-hemorrhagic in women with heavy menstrual blood loss (menorrhagia). However, prior to this study, there were no scientific investigations to support this fact, because the aqueous extract from Brownea grandiceps flowers had not been previously evaluated neither phytochemically nor biologically. The objective of this work was to evaluate in vitro the effects of aqueous extract from Brownea grandiceps flowers on the coagulation system and fibrinolysis. An infusion of Brownea grandiceps flowers (160g) was performed; then, it was homogenized, centrifuged and lyophilized to obtain the aqueous extract, and this was called BGE. Subsequently, the extract was characterized on the one hand, phytochemically and on the other hand, biologically, employing prothrombin time (PT), partial thromboplastin time (PTT) and thrombin time (TT) to determine the effects on extrinsic, intrinsic and common coagulation pathways, respectively. In addition to that, the fibrinogenolytic and fibronectinase activity was evaluated by SDS-PAGE using Tris-Tricine system and analyzed by densitometric study utilizing ImageJ program. Also, by using specific chromogenic substrates for Factor Xa (FXa), thrombin, tissue plasminogen activator (t-PA), urokinase plasminogen activator (u-PA) and plasmin, it was assessed whether BGE exhibited some enzyme-like activity, and inhibitory activity of the afore mentioned enzymes. Fibrinolytic and antifibrinolytic activities were determined by a fibrin plate method. Data were analyzed by an nonparametric method. BGE presented tannins, saponins, glycosides, alkaloids, flavonoids, coumarins, and did not contain triterpenoids and steroids. Also, BGE at low concentrations (250-1250µg/mL) reduced the PT, while higher concentrations (15000-25000µg/mL) prolonged this time. However, BGE concentrations between 1250 and 25000µg/mL prolonged the PTT. Prolongation of PT and PTT was observed at high

  20. Combretum lanceolatum flowers extract shows antidiabetic activity through activation of AMPK by quercetin

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    Carlos Roberto Porto Dechandt

    2013-04-01

    Full Text Available The present study evaluated the antidiabetic activity of the Combretum lanceolatum Pohl ex Eichler, Combretaceae, flowers extract (ClEtOH in diabetic rats. Streptozotocin-diabetic rats were divided into four groups: diabetic control, diabetic treated with 500 mg/kg of metformin and diabetic treated with 250 or 500 mg/kg of ClEtOH for 21 days. The treatment of diabetic rats with 500 mg/kg of ClEtOH promoted an increase in the weight of liver, white adipose tissues and skeletal muscles, improving body weight gain. Diabetic rats treated with 500 mg/kg of ClEtOH also presented reduction in glycemia, glycosuria and urinary urea levels, and increase in liver glycogen content. HPLC chromatogram showed that quercetin is the major compound in the extract. The phosphorylation levels of adenosine monophosphate-activated protein kinase were increased in liver slices incubated in vitro with 50 µg/mL of ClEtOH, similarly to the incubation with metformin (50 µg/mL or quercetin (10 µg/mL. The antihyperglycemic effect of ClEtOH was similar to that of metformin and appears to be through inhibition of gluconeogenesis, since urinary urea was reduced and skeletal muscle mass was increased. These data indicate that the antidiabetic activity of the Combretum lanceolatum extract could be mediated, at least in part, through activation of adenosine monophosphateactivated protein kinase by quercetin.

  1. Comparative analysis of antioxidant, antimicrobiological and cytotoxic activities of native and fermented chamomile ligulate flower extracts.

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    Cvetanović, Aleksandra; Švarc-Gajić, Jaroslava; Zeković, Zoran; Savić, Saša; Vulić, Jelena; Mašković, Pavle; Ćetković, Gordana

    2015-09-01

    The work investigated differences in apigenin content, as well as in other compounds, and examined the chemical profiles, antioxidant, antimicrobial and cytotoxic effects of extracts obtained from native and fermented chamomile ligulate flowers. Chamomile (Chamomilla recutita L.) has a long history of being used as a medicinal plant due to many health benefits, including antiinflammatory, anticancer, antispasmodic, radical-scavenging effects and others. Apigenin is recognized as one of the most bioactive phenolic compounds in chamomile. In comparison to its bound forms, which include mostly apigenin-7-O-β-glucoside and various acylated forms, the aglycone is attributed with much higher bioactivity. Due to this fact, in this work ligulate florets of chamomile anthodium were subjected to a fermentation process using native chamomile enzymes to hydrolyze bound forms of apigenin to free aglycone. The contents of apigenin and apigenin-7-O-β-glucoside were determined in both fermented and nonfermented samples by UHPLC-MS-MS analysis to define the efficiency of conversion. After defining their chemical profiles, the extracts of fermented and nonfermented chamomile samples were also compared with respect to their antioxidant, antimicrobial and cytotoxic effects. The antioxidant effects of the obtained extracts were defined by electron spin resonance analysis for hydroxyl and superoxide radicals. The antimicrobial activity was defined for eight microbial strains, whereas cytotoxic activity was evaluated using two human cell lines (human cervix carcinoma and human rhabdomyosarcoma) and murine fibroblasts.

  2. Combretum lanceolatum flowers extract shows antidiabetic activity through activation of AMPK by quercetin

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    Carlos Roberto Porto Dechandt

    2012-12-01

    Full Text Available The present study evaluated the antidiabetic activity of the Combretum lanceolatum Pohl ex Eichler, Combretaceae, flowers extract (ClEtOH in diabetic rats. Streptozotocin-diabetic rats were divided into four groups: diabetic control, diabetic treated with 500 mg/kg of metformin and diabetic treated with 250 or 500 mg/kg of ClEtOH for 21 days. The treatment of diabetic rats with 500 mg/kg of ClEtOH promoted an increase in the weight of liver, white adipose tissues and skeletal muscles, improving body weight gain. Diabetic rats treated with 500 mg/kg of ClEtOH also presented reduction in glycemia, glycosuria and urinary urea levels, and increase in liver glycogen content. HPLC chromatogram showed that quercetin is the major compound in the extract. The phosphorylation levels of adenosine monophosphate-activated protein kinase were increased in liver slices incubated in vitro with 50 µg/mL of ClEtOH, similarly to the incubation with metformin (50 µg/mL or quercetin (10 µg/mL. The antihyperglycemic effect of ClEtOH was similar to that of metformin and appears to be through inhibition of gluconeogenesis, since urinary urea was reduced and skeletal muscle mass was increased. These data indicate that the antidiabetic activity of the Combretum lanceolatum extract could be mediated, at least in part, through activation of adenosine monophosphateactivated protein kinase by quercetin.

  3. Design, Formulation and Evaluation of an Oral Gel from Punica Granatum Flower Extract for the Treatment of Recurrent Aphthous Stomatitis

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    Abolfazl Aslani

    2016-09-01

    Full Text Available Purpose: Recurrent aphthous stomatitis is a disease with unknown etiology that’s mostly treated symptomatically and has no definite cure. Pomegranate (Punica granatum flowers have been used as medicinal herb that due to its antimicrobial, antioxidant, anti-inflammatory, analgesic and healing effects, has been useful in treatment of oral aphthous. Therefore, we decided to formulate a mucoadhesive gel with pomegranate flower extract to reduce the need for corticosteroid therapy in patients. Methods: Pomegranate flowers are extracted by percolation method. Several formulations with different amounts of carbomer 934, sodium carboxymethylcellulose (SCMC and hydroxypropyl methylcellulose K4M were prepared and the condensed extract was dispersed in polyethyleneglycol (PEG 400 and added to gel bases. Then the formulations underwent macroscopic and microscopic studies. The formulations that passed these tests successfully were studied through assay tests using spectrophotometry in 765 nm, drug release from mucoadhesive gel using cell diffusion method, viscosity test, mucoadhesion test and accelerated stability test. Results: The phenolic content of pomegranate flower dried extract was found to be 212.3±1.4 mg/g in dried extract. The F4–F6 formulations contains carbomer 934, SCMC, pomegranate flower extract, PEG 400, potassium sorbate and purified water passed all above tests. Conclusion: The F4 formulation had higher viscosity and mucoadhesion values due to its higher carbomer 934 and SCMC content. Since F4, F5 and F6 had no significant variation in drug release, the F4 formulation was chosen as the superior formulation because of proper appearance and uniformity, acceptable viscosity, mucoadhesion and stability in different temperatures.

  4. In vitro anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves.

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    Nikmehr, Banafsheh; Ghaznavi, Habib; Rahbar, Amir; Sadr, Samira; Mehrzadi, Saeed

    2014-06-01

    The anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves against extracellular (promastigote) and intracellular (amastigote) forms of Leishmania major were evaluated in this study. In the first stage, promastigote forms of L. major, were treated with different doses of the plant extracts in a 96-well tissue-culture microplate and IC50 values for each extract were measured with colorimetric MTT assay. In the second stage, macrophage cells were infected with L. major promastigotes. Infected macrophages were treated with plant extracts. Then the macrophages were stained with Gimsa and the number of infected macrophages and amastigotes were counted with a light microscope. The results indicated that the plant extracts inhibited the growth of promastigotes and amastigotes of L. major. Inhibitory concentrations (IC50) for promastigote assay were 108.19, 155.15, and 184.32 μgmL(-1) for C. officinalis flowers, D. stramonium seeds and S. officinalis, respectively. The extracts also reduced the number of amastigotes in macrophage cells from 264 for control group to 88, 97, and 102 for test groups. Although the anti-leishmanial activity of the extracts were not comparable with the standard drug, miltefosine; but they showed significant efficiency in reducing the number of amastigotes in macrophages, in comparison with the control group (P officinalis flowers, D. stramonium seeds, and S. officinalis leaves to control of cutaneous leishmaniasis. Copyright © 2014 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  5. Investigation of the interactions between Chrysanthemum morifolium flowers extract and intestinal bacteria from human and rat.

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    Tao, Jin-Hua; Duan, Jin-Ao; Qian, Yi-Yun; Qian, Da-Wei; Guo, Jian-Ming

    2016-11-01

    Flos Chrysanthemi, dried flower of Chrysanthemum morifolium Ramat, has drawn much attention recently owing to its potential beneficial health effects for human. Flos Chrysanthemi products are usually taken orally and metabolized by intestinal microflora. However, there has been no investigation of the comprehensive metabolic profile of the Flos Chrysanthemi extract by intestinal flora owing to its chemical complexity and the limitations of analytical methods. In this paper, a rapid, sensitive and automated analysis method, ultra-performance liquid chromatography/quadrupole time of flight mass spectrometry including MS E technology and automated data processing Metabolynx™ software, was developed and successfully applied for the biotransformation and metabolic profile of flavonoids in the Flos Chrysanthemi extract by intestinal flora from human and rat. A total of 32 metabolites were detected and tentatively identified in human and rat intestinal bacterial samples. These metabolites indicated that hydrolysis, hydroxylation, acetylation, methylation, hydrogenation and deoxygenation were the major conversion pathways of flavonoids in the Flos Chrysanthemi extract in vitro. Furthermore, the effects of the Flos Chrysanthemi extract on the growth of different intestinal bacteria were detected using an Emax precision microplate reader. Certain pathogenic bacteria such as Enterobacter, Enterococcus, Clostridium and Bacteroides were significantly inhibited by Flos Chrysanthemi, while commensal probiotics such as Lactobacillus and Bifidobacterium were moderately promoted. Our observation provided further evidence for the importance of intestinal bacteria in the metabolism and potential activity of the Flos Chrysanthemi extract. The results will also be helpful for the further pharmacokinetic study of Flos Chrysanthemi and to unravel how it works in vivo. Copyright © 2016 John Wiley & Sons, Ltd.

  6. An in vivo and in vitro investigation of the effect of Aloe vera gel ethanolic extract using animal model with diabetic foot ulcer

    Directory of Open Access Journals (Sweden)

    Mohan Daburkar

    2014-01-01

    Full Text Available Aim: To examine the preventive effect of Aloe vera gel ethanolic extract using diabetic foot ulcer (DFUs protocol in Wistar rats. Materials and Methods: Male Wistar rats were divided into untreated control (Group I, untreated DFUs (Group II, DFUs treated with A. vera gel ethanolic extract (Group III, DFUs treated with topical A. vera gel (Group IV, DFUs treated with A. vera gel ethanolic extract and topical A. vera gel (Group V. The rats in the treatment groups were daily administered the A. vera gel and ethanolic extract for 9 days. Fasting blood glucose levels and percentage of wound ulcer contraction were measured on day 3, 6, and 9. Statistical Analysis used: The results are expressed as a mean ± Standard Error Mean (SEM. Data were analyzed using one-way analysis of variance (ANOVA after Newman-Keuls test. P < 0.05 were considered statistically significant in all cases. Results: Oral administration of A. vera gel ethanolic extract at a dose of 300 mg/kg body weight per day to diabetic rats for a period of 9 days resulted in a significant reduction in fasting blood glucose and a significant improvement in plasma insulin. Topical application of A. vera gel at a dose 30 mg/kg body weight per day to streptozotocin (STZ-induced diabetic rats for a period of 9 days resulted in no change in blood glucose and plasma insulin. Oral administration as well as topical application of A. vera gel ethanolic extract and gel significantly reduced the blood glucose, improved the plasma insulin, and significantly increased DNA and glycosaminoglycans (GAGs to improve the wound ulcer healing as well as the breaking strength on day 9. Conclusions: Present findings provide a scientific rationale for the use of A. vera gel ethanolic extract, and showed that the gel attenuated the diabetic foot wound in rats.

  7. On the novel action of melanolysis by a leaf extract of Aloe vera and its active ingredient aloin, potent skin depigmenting agents.

    Science.gov (United States)

    Ali, Sharique A; Galgut, Jyoti M; Choudhary, Ram K

    2012-05-01

    The present study was carried out to investigate the effects of an Aloe vera leaf extract, along with its standard active ingredient aloin, on the isolated tail melanophores of Bufo melanostictus tadpoles, which are a type of disguised smooth muscle cells offering excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. It was found that the leaf extract of A. vera and its active ingredient aloin induced powerful, dose-dependent, physiologically significant melanin aggregating effects in the isolated tail melanophores of B. melanostictus similar to those of adrenaline per se. These preliminary findings clearly demonstrate that the extract of A. vera and its active ingredient aloin cause melanin aggregation leading to skin lightening via alpha adrenergic receptor stimulation. The present study opens new vistas for the use of A. vera regarding its clinical application as a new nontoxic melanolytic agent for the treatment of hyperpigmentation. Georg Thieme Verlag KG Stuttgart · New York.

  8. Comparative evaluation of antimicrobial activity of hydroalcoholic extract of Aloe vera, garlic, and 5% sodium hypochlorite as root canal irrigants against Enterococcus faecalis: An in vitro study.

    Science.gov (United States)

    Karkare, Swati Ramesh; Ahire, Nivedita Pramod; Khedkar, Smita Uday

    2015-01-01

    Enterococcus faecalis are the most resistant and predominant microorganisms recovered from root canals of teeth where previous treatment has failed. Over the past decade, interest in drugs derived from medicinal plants has markedly increased. In dentistry, phytomedicines has been used as an anti-inflammatory, antibiotic, analgesic, sedative, and also as an endodontic irrigant. In endodontics, because of the cytotoxic reactions of most of the commercial intracanal medicaments and their inability to eliminate bacteria completely from dentinal tubules, the trend is shifting toward use of biologic medication extracted from natural plants. To compare the antimicrobial efficacy of newer irrigating agents which would probably be as effective or more and at the same time less irritating to the tissues than sodium hypochlorite (NaOCl). The objective of this study was to compare the antimicrobial activity of saturated and diluted (1:1) hydroalcoholic extract of Aloe vera, garlic, and 5% NaOCl against E. faecalis using the commonly used agar diffusion method. Saturated hydroalcoholic extract of A. vera showed the highest zone of inhibition against E. faecalis. NaOCl, which is considered as gold standard, also showed higher zones of inhibition.

  9. Comparative evaluation of antimicrobial activity of hydroalcoholic extract of Aloe vera, garlic, and 5% sodium hypochlorite as root canal irrigants against Enterococcus faecalis: An in vitro study

    Directory of Open Access Journals (Sweden)

    Swati Ramesh Karkare

    2015-01-01

    Full Text Available Introduction: Enterococcus faecalis are the most resistant and predominant microorganisms recovered from root canals of teeth where previous treatment has failed. Over the past decade, interest in drugs derived from medicinal plants has markedly increased. In dentistry, phytomedicines has been used as an anti-inflammatory, antibiotic, analgesic, sedative, and also as an endodontic irrigant. In endodontics, because of the cytotoxic reactions of most of the commercial intracanal medicaments and their inability to eliminate bacteria completely from dentinal tubules, the trend is shifting toward use of biologic medication extracted from natural plants. Aim: To compare the antimicrobial efficacy of newer irrigating agents which would probably be as effective or more and at the same time less irritating to the tissues than sodium hypochlorite (NaOCl. The objective of this study was to compare the antimicrobial activity of saturated and diluted (1:1 hydroalcoholic extract of Aloe vera, garlic, and 5% NaOCl against E. faecalis using the commonly used agar diffusion method. Results: Saturated hydroalcoholic extract of A. vera showed the highest zone of inhibition against E. faecalis. NaOCl, which is considered as gold standard, also showed higher zones of inhibition.

  10. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species.

    Science.gov (United States)

    Gordanian, B; Behbahani, M; Carapetian, J; Fazilati, M

    2014-01-01

    The present study was carried out to investigate cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species against breast cancer cell line (MCF7) and human embryonic kidney normal cell line (HEK293). The studied Artemisia species were A. absinthium, A. vulgaris, A. incana, A. fragrans and A. spicigera. The cytotoxic activity was measured by MTT assay at different concentrations (62.5, 125, 250, 500 μg/ml). Among these five species, methanol extracts of flower, leaf, stem and root of A. absinthium and A. vulgaris exhibited considerable cytotoxic activity. The flower extracts of these two species were found to have higher cytotoxic effect on MCF7 cell with an IC50 value of 221.5 and >500 μg/ml, respectively. Leaf methanol extract of A. incana also showed cytotoxic activity. Cytotoxic activity of different extracts of A. absinthium, A. vulgaris and A. incana against MCF7 was 10%-40% more than HEK293 cells. Not only the extracts of A. spicigera and A. fragrans did not show any cytotoxic effect against both cell lines, but also increased the number of cells. This study revealed that A. absinthium and A. vulgaris may have a great potential to explore new anticancer drugs.

  11. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species

    Science.gov (United States)

    Gordanian, B.; Behbahani, M.; Carapetian, J.; Fazilati, M.

    2014-01-01

    The present study was carried out to investigate cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species against breast cancer cell line (MCF7) and human embryonic kidney normal cell line (HEK293). The studied Artemisia species were A. absinthium, A. vulgaris, A. incana, A. fragrans and A. spicigera. The cytotoxic activity was measured by MTT assay at different concentrations (62.5, 125, 250, 500 μg/ml). Among these five species, methanol extracts of flower, leaf, stem and root of A. absinthium and A. vulgaris exhibited considerable cytotoxic activity. The flower extracts of these two species were found to have higher cytotoxic effect on MCF7 cell with an IC50 value of 221.5 and >500 μg/ml, respectively. Leaf methanol extract of A. incana also showed cytotoxic activity. Cytotoxic activity of different extracts of A. absinthium, A. vulgaris and A. incana against MCF7 was 10%-40% more than HEK293 cells. Not only the extracts of A. spicigera and A. fragrans did not show any cytotoxic effect against both cell lines, but also increased the number of cells. This study revealed that A. absinthium and A. vulgaris may have a great potential to explore new anticancer drugs. PMID:25657777

  12. Effects of ethanolic extract ofJasminum grandiflorumLinn. flowers on wound healing in diabeticWistaralbino rats.

    Science.gov (United States)

    Hirapara, Hiren; Ghori, Vishal; Anovadiya, Ashish; Baxi, Seema; Tripathi, Chandrabhanu

    2017-01-01

    To evaluate wound healing activity of ethanolic extract of Jasminum grandiflorum Linn. ( J. grandiflorum ) flowers in diabetic rats. Streptozotocin-induced diabetic Wistar albino rats were divided into six groups (n=6).Three groups - diabetic control, positive control (that received Glibenclamide) and treatment (that received J. grandiflorum Linn. Flower extract) were operated for excision wounds (EW). These groups were evaluated for wound contraction and re-epithelization. The other three groups were operated for incision wounds (IW) and dead space wounds (DW). Incision and dead space wounds were produced in the same rats. IWs were analyzed for wound breaking strength and the granulation tissues from DWs were analyzed for dry weight, hydroxyproline content, and histology. IWs and DWs showed significant improvement in wound breaking strength (265.8±10.4 vs 332.5±8.2; pday 14) in treatment group compared to diabetic control (day 20). No significant improvement was seen in re-epithelization in treatment group. Ethanolic extract of J. grandiflorum Linn. flowers increases granulation tissue formation as well as neo-angiogenesis. It also enhances wound contraction; however, re-epithelization was not significantly affected. J. grandiflorum Linn. flowers could be potentially effective in promotion of diabetic wounds healing by increasing granulation tissue formation and enhancing wound contraction; however, further studies are required for its clinical application.

  13. Prokinetic Activity of Prunus persica (L. Batsch Flowers Extract and Its Possible Mechanism of Action in Rats

    Directory of Open Access Journals (Sweden)

    Wei Han

    2015-01-01

    Full Text Available The peach tree, Prunus persica (L. Batsch, is widely cultivated in China, and its flowers have been used for centuries in traditional Chinese medicine to treat gut motility disorders. But few studies have explored the pharmacological effect of Prunus persica (L. Batsch flowers on gastrointestinal motility. In this study, the activities of different extracts from Prunus persica (L. Batsch flowers on the smooth muscle contractions were evaluated using isolated colon model, and the ethyl acetate extract (EAE showed the strongest effects in vitro. EAE (10−8–10−5 g/mL caused a concentration-dependent stimulatory effect in rat colonic tissue. Additionally, ketotifen (100 µM, cimetidine (10 µM, and pyrilamine (1 µM produced a significant inhibition of contractions caused by EAE. Furthermore, immunofluorescence and toluidine blue staining revealed increased numbers of mast cells in the EAE group, and EAE increased histamine release from the colonic tissues. These data indicate that EAE has significant prokinetic activity and acts by a mechanism that mainly involves mast cell degranulation. Our study provides a pharmacological basis for the use of an extract of Prunus persica (L. Batsch flowers in the treatment of gut motility disorders.

  14. Cytotoxicity study of plant Aloe vera (Linn

    Directory of Open Access Journals (Sweden)

    Atul N Chandu

    2012-01-01

    Full Text Available Background: The objective of this study has been to evaluate the in-vitro antitumor activity of Aloe vera extract of in cultured B16F10 melanoma cell line by measuring cell viability using "Trypan blue exclusion assay" method. Aim: To find out such kind of anticancer drug which is a cheap, safe, less toxic, and more potent drug compared to chemotherapy drug. Materials and Methods: In-vitro antitumor activity cell culture1, drug treatment (standard and test extract and Trypan blue exclusion assay growth and viability test 1 were used. Treatment of Aloe vera extract against B16F10 melanoma cell line, in all concentration range, showed decrease in percent cell viability, as compared to that of negative when examined by "Trypan blue exclusion assay". Results: In overall variation of test samples, Aloe vera extract showed its best activity in the concentration of 300 μg/ml, which was approximately equal to the activity of standard drug doxorubicin. Evaluation of in-vitro antitumor activity revealed that Aloe vera extract exhibits good cytotoxic activity. The best cytotoxic activity by Aloe vera was shown at 200 μg/ml concentration. Conclusion: The study of cytoprotection against normal cells by micronucleus assay has shown that the herbal extracts have less toxic effects to the normal blood lymphocytes, as compared to that of standard anticancer drug.

  15. Antifungal activity of aloe vera gel against plant pathogenic fungi

    International Nuclear Information System (INIS)

    Sitara, U.; Hassan, N.; Naseem, J.

    2011-01-01

    Aloe vera gel extracted from the Aloe vera leaves was evaluated for their antifungal activity at the rate of 0.15%, 0.25% and 0.35% concentration against five plants pathogenic fungi viz., Aspergillus niger, Aspergillus flavus, Alternaria alternata, Drechslera hawaiensis and Penicillum digitatum 0.35% concentration Aloe vera gel completely inhibited the growth of Drechslera hawaiensis and Alternaria alternata. (author)

  16. Extent of Use of Aloe vera Locally Extracted Products for Management of Ailments in Communities of Kitagata Sub-county in Sheema District, Western Uganda

    Science.gov (United States)

    Adams, Kamukama; Eliot, Twineomujuni; Gerald, Agaba

    2015-01-01

    Aloe vera is widely used locally in communities in Uganda as a medicinal plant. It is said to contain various nutrient substances and vitamins that have curative properties. It is said to heal a variety of diseases in various communities. However the extent of use of this potential medicinal plant in Uganda and the various ailments for which it is used and the treatment outcomes are not clearly established and documented. In this cross-sectional study, carried out in August 2012 in Kitagata sub-county in Sheema district in western Uganda, data was collected from 131 randomly selected adult respondents using an interviewer administered semi-structured questionnaire. Key informants interviews and focused group discussions were also carried out with purposively selected participants. Data were collected on social demographic characteristics, practices and beliefs about Aloe vera. The data were analyzed using Excel version 2007 and Epi_Info software. To get the proportion of the community that use Aloe vera, the number of respondents that use aloe vera was expressed as a percentage of the total number of respondents. It was found out that all the respondents (100%) know aloe vera plant, 96.1% think that it can cure and 84.7% have ever used it. 90.9% of the respondents believe that Aloe vera is effective in curing ailments. 82.9% of these strongly believe in Aloe vera’s effectiveness. The diseases reported included malaria (31%), wounds (23%), abdominal pains (16%) and skin diseases (9%) among others. It was significantly noted that all the participants who had ever used Aloe vera still believe in it. 92.0% respondents reported that they can recommend aloe vera to a friend or relative. Only one participant strongly disagrees that Aloe vera has any curative properties and has never used it. PMID:26855960

  17. Anti-amnesic activity of Citrus aurantium flowers extract against scopolamine-induced memory impairments in rats.

    Science.gov (United States)

    Rahnama, Samira; Rabiei, Zahra; Alibabaei, Zahra; Mokhtari, Shiva; Rafieian-Kopaei, Mahmoud; Deris, Fatemeh

    2015-04-01

    Alzheimer's disease (AD) is a progressive neurological disorder that mostly affects the elderly population. Learning and memory impairment as the most characteristic manifestation of dementia could be induced chemically by scopolamine, a cholinergic antagonist. Cholinergic neurotransmission mediated brain oxidative stress. Citrus aurantium (CA) has traditionally been used for the treatment of insomnia, anxiety and epilepsy. The present study was designed to investigate the effect of Citrus aurantium on scopolamine-induced learning and memory deficit in rats. Forty-two Wistar rats were divided into six equal groups. (1) Control (received saline), (2) SCOP (scopolamine at a dose of 1 mg/kg for 15 days), (3) and (4) SCOP + CA (scopolamine and CA extract at doses of 300 and 600 mg/kg per day for 15 days), (5) and (6) intact groups (CA extract at 300 and 600 mg/kg per day for 15 days, respectively). Administration of CA flower extract significantly restored memory and learning impairments induced by scopolamine in the passive avoidance test and also reduced escape latency during trial sessions in the Morris water maze test. Citrus aurantium flower extract significantly decreased the serum malondialdehyde (MDA) levels. Citrus aurantium flower extract has repairing effects on memory and behavioral disorders produced by scopolamine and may have beneficial effects in the treatment of AD.

  18. Facile one step synthesis of novel TiO2 nanocoral by sol-gel method using Aloe vera plant extract

    Science.gov (United States)

    Venkatesh, K. S.; Krishnamoorthi, S. R.; Palani, N. S.; Thirumal, V.; Jose, Sujin P.; Wang, Fu-Ming; Ilangovan, R.

    2015-05-01

    Titanium oxide (TiO2) nanoparticles (NPs) were synthesized by sol gel method using Aloe vera plant extract as a biological capping agent and a cauliflower-nanocoral morphology was observed in this technique. The assynthesized TiO2 nanopowder was calcined at a range of temperatures (300-600 °C) for 1 h. The influence of A. vera plant extract on the thermal, structural and morphological properties of TiO2 nanopowder was evaluated. Thermogravimetric analysis/differential thermal analysis was employed to study the thermal properties of the assynthesized TiO2 nanopowder. The crystallinity, phase transformation and the crystallite size of the calcined samples were studied by X-ray diffraction technique. XRD result confirmed the presence of TiO2 with anatase phase. FT Raman spectra showed the Raman active modes pertaining to the TiO2 anatase phase and Raman band shift was also observed with respect to particle size variation. The different functional group vibrations of as dried pure A. vera plant extract were compared with the mixture of TiO2 and A. vera plant extract by FT-IR analysis. The scanning electron microscopy images apparently showed the formation of spherical shaped NPs and also it demonstrated the effect of A. vera plant extract on the reduction of particles size. The surface area of the TiO2 NPs was measured through Brunauer-Emmett-Teller analysis. Transmission electron microscopy images ascertained that the spherical shaped TiO2 NPs were formed with cauliflower-nanocoral morphology decorated with nanopolyps with the size range between 15 and 30 nm.

  19. The Ethanol Extract of Osmanthus fragrans Flowers Reduces Oxidative Stress and Allergic Airway Inflammation in an Animal Model

    Directory of Open Access Journals (Sweden)

    Chien-Ya Hung

    2013-01-01

    Full Text Available The Osmanthus fragrans flower, a popular herb in Eastern countries, contains several antioxidant compounds. Ben Cao Gang Mu, traditional Chinese medical literature, describes the usefulness of these flowers for phlegm and stasis reduction, arrest of dysentery with blood in the bowel, and stomachache and diarrhea treatment. However, modern evidence regarding the therapeutic efficacy of these flowers is limited. This study was aimed at assessing the antioxidative effects of the ethanol extract of O. fragrans flowers (OFE in vivo and evaluating its antioxidant maintenance and therapeutic effect on an allergic airway inflammation in mice. After OFE’s oral administration to mice, the values obtained in the oxygen radical absorbance capacity assay as well as the glutathione concentration in the lungs and spleens of mice increased while thiobarbituric acid reactive substances decreased significantly, indicating OFE’s significant in vivo antioxidant activity. OFE was also therapeutically efficacious in a mouse model of ovalbumin-induced allergic airway inflammation. Orally administered OFE suppressed ovalbumin-specific IgE production and inflammatory cell infiltration in the lung. Moreover, the antioxidative state of the mice improved. Thus, our findings confirm the ability of the O. fragrans flowers to reduce phlegm and suggest that OFE may be useful as an antiallergic agent.

  20. Flowering Trees

    Indian Academy of Sciences (India)

    The female flowers are drooping and are larger than male flowers. Fruit is large, red in color and velvety. Seeds are flattened, smooth and reddish-brown. The unripe fruit is astringent and its extract is used in medicine. It is also used as a tan for fishing-nets. The viscid pulp is used as glue for bookbinding and also as a tar for ...

  1. In-vitro Antioxidant, Cytotoxic, Cholinesterase Inhibitory Activities and Anti-Genotoxic Effects of Hypericum retusum Aucher Flowers, Fruits and Seeds Methanol Extracts in Human Mononuclear Leukocytes.

    Science.gov (United States)

    Keskin, Cumali; Aktepe, Necmettin; Yükselten, Yunus; Sunguroglu, Asuman; Boğa, Mehmet

    2017-01-01

    The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of H. retusum only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the in-vitro and in-vivo the benefits of H. retusum seed methanol extracts.

  2. Response surface optimization of ultrasound-assisted flavonoids extraction from the flower of Citrus aurantium L. var. amara Engl.

    Science.gov (United States)

    Yang, Li; Cao, Ya-Lan; Jiang, Jian-Guo; Lin, Qing-Sheng; Chen, Jian; Zhu, Liang

    2010-05-01

    Citrus aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus aurantium L. var. amara Engl.

  3. Nanocrystalline spinel ferrite (MFe2O4, M = Ni, Co, Mn, Mg, Zn) powders prepared by a simple aloe vera plant-extracted solution hydrothermal route

    International Nuclear Information System (INIS)

    Phumying, Santi; Labuayai, Sarawuth; Swatsitang, Ekaphan; Amornkitbamrung, Vittaya; Maensiri, Santi

    2013-01-01

    Graphical abstract: This figure shows the specific magnetization curves of the as-prepared MFe 2 O 4 (M = Ni, Co, Mn, Mg, Zn) powders obtained from room temperature VSM measurement. These curves are typical for a soft magnetic material and indicate hysteresis ferromagnetism in the field ranges of ±500 Oe, ±1000 Oe, and ±2000 Oe for the CoFe 2 O 4 , MgFe 2 O 4 and MnFe 2 O 4 respectively, whereas the samples of NiFe 2 O 4 and ZnFe 2 O 4 show a superparamagnetic behavior. Highlights: ► Nanocrystalline MFe 2 O 4 powders were synthesized by a novel hydrothermal method. ► Metal acetylacetonates and aloe vera plant-extracted solution are used. ► This biosynthetic route is very simple and provides high-yield oxide nanomaterials. ► XRD and TEM results indicate that the prepared samples have only spinel structure. ► The maximum M s of 68.9 emu/g at 10 kOe were observed for the samples of MnFe 2 O 4 . - Abstract: Nanocrystalline spinel ferrite MFe 2 O 4 (M = Ni, Co, Mn, Mg, Zn) powders were synthesized by a novel hydrothermal method using Fe(acac) 3 , M(acac) 3 (M = Ni, Co, Mn, Mg, Zn) and aloe vera plant extracted solution. The X-ray diffraction and selected-area electron diffraction results indicate that the synthesized nanocrystalline have only spinel structure without the presence of other phase impurities. The crystal structure and morphology of the spinel ferrite powders, as revealed by TEM, show that the NiFe 2 O 4 and CoFe 2 O 4 samples contain nanoparticles, whereas the MnFe 2 O 4 and MgFe 2 O 4 samples consist of many nanoplatelets and nanoparticles. Interestingly, the ZnFe 2 O 4 sample contains plate-like structure of networked nanocrystalline particles. Room temperature magnetization results show a ferromagnetic behavior of the CoFe 2 O 4 , MnFe 2 O 4 and MgFe 2 O 4 samples, whereas the samples of NiFe 2 O 4 and ZnFe 2 O 4 exhibit a superparamagnetic behavior

  4. Approaches to the determination of antioxidant activity of extracts from bee bread and safflower leaves and flowers

    Directory of Open Access Journals (Sweden)

    Nataliia Hudz

    2017-01-01

    Full Text Available The main objective of this study was to develop approaches for the determination of total antioxidant activity of natural products (bee bread and safflower extracts using DPPH radical scavenging assay. Considering that analytical procedures and results related to this assay and reported by many authors are significally differed between each other and depend on many factors (the nature of tested extracts, the nature of solvents for extraction, a reaction time of DPPH with a sample, DPPH solvents and concentration, ratio between DPPH and an extract, etc., the methodology of the evaluation of antioxidant capacity of different origin extracts by DPPH radical scavenging assay was developed. Ascorbic acid (AA was used as standard antioxidant and the correlation between the percentage of DPPH scavenging and AA concentration was determined at two different initial absorbances of DPPH solution. Average concentration of AA which inhibited 50% of DPPH radicals (IC50 was equal to 156.0 - 171.26 µg.mL-1. The reaction kinetics of DPPH inhibition by bee bread and safflower extracts was described by the curves of the dependence of the total antioxidant activity on time with squared correlation coefficients (R2 in the range of 0.89 - 0.98. The reaction times for these extracts were from 40 to 70 min at the correct ratio of volumes between the tested extracts and a DPPH solution. These studies demonstrated that the extracts obtained from bee bread of 2016 year of pollen collection had significantly higher the total antioxidant activity compared with the extracts of bee bread of pollen collection of 2015 considering the ratio of bee bread and the solvent in the extracts and volume of the extract for the procedure. This fact is explained not only botanical origin bun also the time of the storage of bee bread before the preparation of extracts. There was not found significant differences in the total antioxidant activity of extracts from flowers of safflower sown

  5. Anti-nociceptive and anti-inflammatory activities of ethanolic flower extract of Newbouldia laevis in mice and rats

    OpenAIRE

    Y Tanko; B Kamba; MI Saleh; K Y Musa; A Mohammed

    2008-01-01

    Summary: The ethanolic flower extract of Newbouldia laevis was investigated for possible anti-nociceptive and anti-inflammatory effects in rodents. Acetic acid induced writhing (in mice) and formalin tests (in rats) were used to study. The extract caused a significant decrease (P< 0.05), which was not dose a dependent inhibition on acetic acid-induced writhing and the neurogenic pain induced by formalin. The extract at the doses (25, 50 and 100mg/kg) tested showed 59, 71 and 47% inhibition...

  6. A Pilot Study of the Effect of Aloe barbadensis Mill. Extract (AVH200®) in Patients with Irritable Bowel Syndrome: a Randomized, Double-Blind, Placebo-Controlled Study.

    Science.gov (United States)

    Størsrud, Stine; Pontén, Irina; Simrén, Magnus

    2015-09-01

    Few effective treatment options exist for patients with irritable bowel syndrome (IBS), and many patients state the use of aloe vera products reduce their symptoms. The aim of this pilot study was to investigate the effect of Aloe barbadensis Mill. Extract (AVH200®) in adult patients with IBS in a randomized, double-blind, placebo controlled study. Sixty-eight adult patients diagnosed with IBS according to the Rome III criteria were randomized to receive AVH200® or matching placebo for four weeks. Symptom questionnaires were completed on a weekly basis and the patients were asked if they had had adequate relief of their gastrointestinal symptoms. A tendency towards a higher proportion of responders in the aloe vera group (55%) vs. placebo (31%), (p=0.09) was observed, and the proportion of subjects who reported adequate relief at least 50% of the weeks during the treatment period tended to be larger in the aloe vera vs. placebo group (33% vs. 14%; p=0.12). The overall severity of the gastrointestinal symptoms was reduced in the aloe vera group (314+/-83 vs. 257+/-107; p=0.003) but not the placebo group (276+/-88 vs. 253+/-100; NS), without difference between the groups (p=0.10). AVH200® was well tolerated and no serious adverse events were observed. Even though the primary endpoint was not met, AVH200® seems to be a promising treatment option for patients with IBS owing to the positive results seen within the secondary endpoints. This study may have been underpowered to detect a clinically meaningful difference between the treatment groups, and therefore larger randomized, controlled studies are required to confirm these results and to elucidate potential mechanisms explaining its effect.

  7. Gnidia glauca flower extract mediated synthesis of gold nanoparticles and evaluation of its chemocatalytic potential

    Directory of Open Access Journals (Sweden)

    Ghosh Sougata

    2012-05-01

    Full Text Available Abstract Background Novel approaches for synthesis of gold nanoparticles (AuNPs are of utmost importance owing to its immense applications in diverse fields including catalysis, optics, medical diagnostics and therapeutics. We report on synthesis of AuNPs using Gnidia glauca flower extract (GGFE, its detailed characterization and evaluation of its chemocatalytic potential. Results Synthesis of AuNPs using GGFE was monitored by UV-Vis spectroscopy and was found to be rapid that completed within 20 min. The concentration of chloroauric acid and temperature was optimized to be 0.7 mM and 50°C respectively. Bioreduced nanoparticles varied in morphology from nanotriangles to nanohexagons majority being spherical. AuNPs were characterized employing transmission electron microscopy, high resolution transmission electron microscopy. Confirmation of elemental gold was carried out by elemental mapping in scanning transmission electron microscopic mode, energy dispersive spectroscopy and X-ray diffraction studies. Spherical particles of size ~10 nm were found in majority. However, particles of larger dimensions were in range between 50-150 nm. The bioreduced AuNPs exhibited remarkable catalytic properties in a reduction reaction of 4-nitrophenol to 4-aminophenol by NaBH4 in aqueous phase. Conclusion The elaborate experimental evidences support that GGFE can provide an environmentally benign rapid route for synthesis of AuNPs that can be applied for various purposes. Biogenic AuNPs synthesized using GGFE exhibited excellent chemocatalytic potential.

  8. Green synthesis, characterization and catalytic activity of silver nanoparticles using Cassia auriculata flower extract separated fraction

    Science.gov (United States)

    Muthu, Karuppiah; Priya, Sethuraman

    2017-05-01

    Cassia auriculata L., the flower aqueous extract was fractionated by separating funnel using n-hexane (A1), chloroform (A2), ethyl acetate (A3) and triple distilled water (A4). The A4 fraction was concentrated and determined the presence of preliminary phytochemicals such as tannins, flavonoids, glycosides, carbohydrates and polyphenolic compounds. These phytochemical compounds acted as reducing as well as a stabilizing agent in the green synthesis of Ag NPs from aqueous silver ions. Initially, the colour change and UV-vis absorbance surface Plasmon resonance strong, wide band located at 435 nm has confirmed the synthesis of Ag NPs. The X-ray diffraction (XRD) pattern of Ag NPs shows a face-centered cubic crystal structure. The observed values were calculated by Debye-Scherrer equation to theoretical confirms the particle size of 18 nm. The surface morphology of Ag NPs was viewed by HRTEM, the particles are spherical and triangle shapes with sizes from 10 to 35 nm. Further, the Ag NPs was effective catalytic activity in the reduction of highly environmental polluted organic compounds of 4-nitrophenol and methyl orange. The green synthesis of Ag NPs seems to eco-friendly, cost-effective, conventional one spot synthesis and greater performance of catalytic degradation of environmentally polluted organic dyes.

  9. Use of Moringa oleifera Flower Pod Extract as Natural Preservative and Development of SCAR Marker for Its DNA Based Identification.

    Science.gov (United States)

    Gull, Iram; Javed, Attia; Aslam, Muhammad Shahbaz; Mushtaq, Roohi; Athar, Muhammad Amin

    2016-01-01

    The use of Moringa oleifera as natural food preservative has been evaluated in the present study. In addition, for quality assurance, the study has also been focused on the shelf life of product to authenticate the identification of plant by development of DNA based marker. Among the different extracts prepared from flower pods of Moringa oleifera, methanol and aqueous extract exhibited high antibacterial and antioxidant activity, respectively. The high phenolic contents (53.5 ± 0.169 mg GAE/g) and flavonoid contents (10.9 ± 0.094 mg QE/g) were also recorded in methanol and aqueous extract, respectively. Due to instability of bioactive compounds in aqueous extract, methanol extract is considered as potent natural preservative. The shelf life of methanol extract was observed for two months at 4°C under dark conditions. The developed SCAR primers (MOF217/317/MOR317) specifically amplified a fragment of 317 bp from DNA of Moringa oleifera samples collected from different regions of Punjab province of Pakistan. The methanol extract of Moringa oleifera flower pods has great potential to be used as natural preservative and nutraceutical in food industry.

  10. Use of Moringa oleifera Flower Pod Extract as Natural Preservative and Development of SCAR Marker for Its DNA Based Identification

    Directory of Open Access Journals (Sweden)

    Iram Gull

    2016-01-01

    Full Text Available The use of Moringa oleifera as natural food preservative has been evaluated in the present study. In addition, for quality assurance, the study has also been focused on the shelf life of product to authenticate the identification of plant by development of DNA based marker. Among the different extracts prepared from flower pods of Moringa oleifera, methanol and aqueous extract exhibited high antibacterial and antioxidant activity, respectively. The high phenolic contents (53.5±0.169 mg GAE/g and flavonoid contents (10.9±0.094 mg QE/g were also recorded in methanol and aqueous extract, respectively. Due to instability of bioactive compounds in aqueous extract, methanol extract is considered as potent natural preservative. The shelf life of methanol extract was observed for two months at 4°C under dark conditions. The developed SCAR primers (MOF217/317/MOR317 specifically amplified a fragment of 317 bp from DNA of Moringa oleifera samples collected from different regions of Punjab province of Pakistan. The methanol extract of Moringa oleifera flower pods has great potential to be used as natural preservative and nutraceutical in food industry.

  11. Effects of ethanolic extract of Jasminum grandiflorum Linn. flowers on wound healing in diabetic Wistar albino rats

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    Hiren Hirapara

    2017-08-01

    Full Text Available Objective: To evaluate wound healing activity of ethanolic extract of Jasminum grandiflorum Linn. (J. grandiflorum flowers in diabetic rats. Materials and Methods: Streptozotocin-induced diabetic Wistar albino rats were divided into six groups (n=6.Three groups – diabetic control, positive control (that received Glibenclamide and treatment (that received J. grandiflorum Linn. Flower extract were operated for excision wounds (EW. These groups were evaluated for wound contraction and re-epithelization. The other three groups were operated for incision wounds (IW and dead space wounds (DW. Incision and dead space wounds were produced in the same rats. IWs were analyzed for wound breaking strength and the granulation tissues from DWs were analyzed for dry weight, hydroxyproline content, and histology. Results: IWs and DWs showed significant improvement in wound breaking strength (265.8±10.4 vs 332.5±8.2; p

  12. Medicinal Mascarene Aloes: An audit of their phytotherapeutic potential.

    Science.gov (United States)

    Lobine, D; Cummins, I; Govinden-Soulange, J; Ranghoo-Sanmukhiya, M; Lindsey, K; Chazot, P L; Ambler, C A; Grellscheid, S; Sharples, G; Lall, N; Lambrechts, I A; Lavergne, C; Howes, M-J R

    2018-01-01

    A phytochemical and biological investigation of the endemic Mascarene Aloes (Aloe spp.), including A. tormentorii (Marais) L.E.Newton & G.D.Rowley, A. purpurea Lam, A. macra Haw., A. lomatophylloides Balf.f and A. vera (synonym A. barbadensis Mill.), which are used in the traditional folk medicine of the Mascarene Islands, was initiated. Methanolic extracts of the Aloes under study were analysed using high resolution LC-UV-MS/MS and compounds belonging to the class of anthraquinones, anthrones, chromones and flavone C-glycosides were detected. The Mascarene Aloes could be distinguished from A. vera by the absence of 2″-O-feruloylaloesin and 7-O-methylaloeresin. GC-MS analysis of monosaccharides revealed the presence of arabinose, fucose, xylose, mannose and galactose in all the Mascarene Aloes and in A. vera. The crude extracts of all Aloes analysed displayed antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Only extracts of A. macra were active against P. aeruginosa and Klebsiella pneumoniae, while none of the Aloe extracts inhibited Propionibacterium acnes. A. macra displayed anti-tyrosinase activity, exhibiting 50% inhibition at 0.95mg/mL, and extracts of A. purpurea (Mauritius) and A. vera displayed activity in a wound healing-scratch assay. In vitro cytotoxicity screening of crude methanolic extracts of the Aloes, using the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) showed that only A. purpurea (Réunion) elicited a modest toxic effect against HL60 cells, with a percentage toxicity of 8.2% (A. purpurea-Réunion) and none of the Aloe extracts elicited a toxic effect against MRC 5 fibroblast cells at a concentration of 0.1mg/mL. Mascarene Aloe species possess noteworthy pharmacological attributes associated with their rich phytochemical profiles. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Ultrasound-assisted dynamic extraction of valuable compounds from aromatic plants and flowers as compared with steam distillation and superheated liquid extraction.

    Science.gov (United States)

    Roldán-Gutiérrez, Jannat M; Ruiz-Jiménez, J; Luque de Castro, M D

    2008-06-15

    A method for the extraction of valuable compounds from plants and flowers (viz. laurel, rosemary, thyme, oregano and tuberose) is proposed. The dynamic approach allows go-and-backward circulation of the extractant (ethanol) through the solid sample subjected to the action of an ultrasound probe (thus reducing sample amount and avoiding overpressure). A multivariate optimisation study and application of the optimum values of the variables to kinetics studies show that 10 min is sufficient to obtain extraction efficiencies that greatly surpass those provided by steam distillation for essential oils or superheated liquid extraction for these oils and other valuable compounds, with lower costs and higher quality of the extract. The extraction time of the proposed method is 176-165 min shorter than steam distillation and 31-20 min shorter than superheated liquid extraction, depending on the target compound.

  14. EXAFS analysis of cations distribution in structure of Co1-xNixFe2O4 nanoparticles obtained by hydrothermal method in aloe vera extract solution

    Science.gov (United States)

    Wongpratat, Unchista; Maensiri, Santi; Swatsitang, Ekaphan

    2016-09-01

    Effect of cations distribution upon EXAFS analysis on magnetic properties of Co1-xNixFe2O4 (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles prepared by the hydrothermal method in aloe vera extract solution were studied. XRD analysis confirmed a pure phase of cubic spinel ferrite of all samples. Changes in lattice parameter and particle size depended on the Ni content with partial substitution and site distributions of Co2+, Ni2+ ions of different ionic radii at both tetrahedral and octahedral sites in the crystal structure. Particle sizes of samples estimated by TEM images were found to be in the range of 10.87-62.50 nm. The VSM results at room temperature indicated the ferrimagnetic behavior of all samples. Superparamagnetic behavior was observed in NiFe2O4 sample. The coercivity (Hc) and remanance (Mr) values were related to the particle sizes of samples. The saturation magnetization (Ms) was increased by a factor of 1.4 to a value of 57.57 emu/g, whereas the coercivity (Hc) was decreased by a factor of 20 to a value of 63.15 Oe for a sample with x = 0.75. In addition to the cations distribution, the increase of aspect ratio (surface to volume ratio) due to the decrease of particle size could significantly affect the magnetic properties of the materials.

  15. Green route for preparing extra-small TiO2 nanoparticles using aloe extracts and their application in degradation of methyl orange

    Science.gov (United States)

    Yin, Fang; Jiang, Bo; Chen, Chunfeng; Fu, Yunzhi; Chen, Yongjun; Xue, Xinghua; Mazidi, Mohsen

    2015-01-01

    TiO2 nanoparticles (NPs) with high-photocatalysis properties both in ultraviolet and visible-light regions were synthesized via a simple sol-gel method, and an aloe leaf extract (ALE) was used as the cap. When the Ti)∶)CHOH∶ALE volume ratio was equal to 0.5∶8∶40, the reaction time was 6 h, and crystallization occurred at 80°C for 2 h, the TiO2NPs had been synthesized. XRD analysis showed that the structure of TiO2NPs without thermal treatment was anatase. Transmission electron microscopy images confirmed their average size, which was around 5 nm. The products showed an excellent photocatalytic property for methyl orange in the presence of UV and sunlight with the characterization of UV-vis. The photocatalytic activity was optimized by adjusting the amount of TiO2, the pH value, and the calcination temperature. Results indicated that ALE acted as a cap and nano-TiO2 could be synthesized without calcination.

  16. Comparison of the Effects of Aqueous Aloe vera Extract and Theophylline on the Histological Changes of Kidney and Renal Factors in Female Rats with Asthma

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    Nahid Gholizade

    2016-09-01

    Full Text Available Background and Objectives: Asthma is a kind of respiratory disease for which different chemical and herbal drugs are used. This study investigated the side effects of theophylline (TEO and Aloe vera extract (AVE on kidney tissue in rats with asthma. . Methods: Thirty six female rats were divided into six groups of six each. Asthma was induced in the rats using acetic acid spray for 12 weeks. At completion of this period, heart blood samples were collected to conduct biochemical tests. Kidney tissue was fixed in formalin 10% and stained with hematoxylin and eosin for microscopic examinations. The data were analyzed by variance and Tukey's test. Results: Inflammatory cells were seen in connective tissue and around renal tubules. In TEO-treated group, mild change in  density of mesenchymal stem cells, atrohpy of glumeral capillaries, and increase in Bowman space were seen, but in AVE (100mg/kg-treated group, kidney improved. The mean creatinine and urea concentrations increased in rats with asthma and TEO and AVE (200mg/kg-treated rats compared to the controls (p<0.05 but significantly decreased in AVE (100mg/kg-treated rats (p<0.05. Conclusion: AVE (100mg/kg is effective in reducing inflammation of the kidney and concentrations of urea and creatinine.

  17. THE EFFECT OF ALOE VERA ON GASTRIC ACID SECRETION ...

    African Journals Online (AJOL)

    The effect of varying doses of ethanol extract of Aloe vera (Liliaceae) on acute gastric mucosal lesions induced by 0.6M HCl and acid output was studied in the pylorus ligated and lumen perfuse rats respectively. Acid secretion was determined by titration of the collected gastric juice to pH 7.0. Intraperitoneal injection of Aloe ...

  18. Antimutagenic and potential anticarcinogenic activities of aloe-vera ...

    African Journals Online (AJOL)

    The study was carried out to verify the potential anticarcinogenic and antimutagenic activity of garlic and aloe-vera. The ability of aqueous garlic extract and Aloe-Vera gel to inhibit mutation in tester strain of Escherichia coli WP2 uvrA was determined in this study. (The tester E. coli tryptophan auxotroph strain was obtained ...

  19. Aloe Vera: The Miracle Plant Its Medicinal and Traditional Uses in India

    OpenAIRE

    R. Rajeswari; M. Umadevi; C. Sharmila Rahale; R.Pushpa; S. Selvavenkadesh; K. P. Sampath Kumar; Debjit Bhowmik

    2012-01-01

    Aloe vera is the oldest medicinal plant ever known and the most applied medicinal plant worldwide. Extracts of Aloe Vera is a proven skin healer. Aloe Vera help to soothe skin injuries affected by burning, skin irritations, cuts and insect bites, and its bactericidal properties relieve itching and skin swellings. It is known to help slow down the appearance of wrinkles and actively repair the damaged skin cells that cause the visible signs of aging. Aloe is a powerfuldetoxifier, antiseptic an...

  20. Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica.

    Science.gov (United States)

    Hu, Wen; Guo, Ting; Jiang, Wen-Jun; Dong, Guang-Li; Chen, Da-Wei; Yang, Shi-Lin; Li, He-Ran

    2015-06-01

    The present study was designed to establish and optimize a new method for extracting chlorogenic acid and cynaroside from Lonicera japonica Thunb. through orthogonal experimental designl. A new ultrahigh pressure extraction (UPE) technology was applied to extract chlorogenic acid and cynaroside from L. japonica. The influential factors, including solvent type, ethanol concentration, extraction pressure, time, and temperature, and the solid/liquid ratio, have been studied to optimize the extraction process. The optimal conditions for the UPE were developed by quantitative analysis of the extraction products by HPLC-DAD in comparison with standard samples. In addition, the microstructures of the medicinal materials before and after extraction were studied by scanning electron microscopy (SEM). Furthermore, the extraction efficiency of different extraction methods and the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of the extracts were investigated. The optimal conditions for extracting chlorogenic acid and cynaroside were as follows: ethanol concentration, 60%; extraction pressure, 400 MPa; extraction time, 2 min; extraction temperature, 30 °C; and the solid/liquid ratio, 1 : 50. Under these conditions, the yields of chlorogenic acid and cynaroside were raised to 4.863% and 0.080%, respectively. Compared with other extraction methods, such as heat reflux extraction (HRE), ultrasonic extraction (UE), and Sohxlet extraction (SE), the UPE method showed several advantages, including higher extraction yield, shorter extraction time, lower energy consumption, and higher purity of the extracts. This study could help better utilize L. japonica flower buds as a readily accessible source of natural antioxidants in food and pharmaceutical industries. Copyright © 2015 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  1. Phenolic acids in extracts obtained from the flowering herbs of Cirsium vulgare (Savi Ten. growing in Poland

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    Małgorzata Kozyra

    2013-12-01

    Full Text Available In this work the phenolic acids in the methanol extract from the flowering herbs of Cirsium vulgare (Savi Ten. growing in Poland were isolated and identified. The samples containing free phenolic acids and those released after acid and alkaline hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Six phenolic acids such as gallic, protokatechuic, gentisic, hydrobenzoic, vanillic and caffeic acids were detected in the fraction of free phenolic acids of the methanol extract, irrespectively of the method used.

  2. Antimutagenicity and anticarcinogenic effects of gel and latex extracts of Aloe vera cultivated: a comparative study in two cities, Iran

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    Mehrabian Sedigheh

    2012-03-01

    Conclusion: Maximum percentage of inhibition was observed in the extracts of the plant cultivated in Karaj reflecting the impact of environmental conditions on the construction of antioxidant compounds in plants.

  3. Components in aqueous Hibiscus rosa-sinensis flower extract inhibit in vitro melanoma cell growth

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    Karina H. Goldberg

    2017-01-01

    Full Text Available Skin cancer is extremely common, and melanoma causes about 80% of skin cancer deaths. In fact, melanoma kills over 50 thousand people around the world each year, and these numbers are rising. Clearly, standard treatments are not effectively treating melanoma, and alternative therapies are needed to address this problem. Hibiscus tea has been noted to have medicinal properties, including anticancer effects. Extracts from Hibiscus have been shown to inhibit the growth of a variety of cancer cells. In particular, recent studies found that polyphenols extracted from Hibiscus sabdariffa by organic solvents can inhibit melanoma cell growth. However, effects of aqueous extracts from Hibiscus rosa-sinesis flowers, which are commonly used to make traditional medicinal beverages, have not been examined on melanoma cells. Here, we report that aqueous H. rosa-sinesis flower extract contains compounds that inhibit melanoma cell growth in a dose dependent manner at concentrations that did not affect the growth of nontransformed cells. In addition, these extracts contain low molecular weight growth inhibitory compounds below 3 kD in size that combine with larger compounds to more effectively inhibit melanoma cell growth. Future work should identify these compounds, and evaluate their potential to prevent and treat melanoma and other cancers.

  4. Effect of Calendula officinalis Flower Extract on Acute Phase Proteins, Antioxidant Defense Mechanism and Granuloma Formation During Thermal Burns

    Science.gov (United States)

    Chandran, Preethi K.; Kuttan, Ramadasan

    2008-01-01

    Effect of Calendula officinalis flower extract was investigated against experimentally induced thermal burns in rats. Burn injury was made on the shaven back of the rats under anesthesia and the animals were treated orally with different doses of the flower extract (20 mg, 100 mg and 200 mg/kg body weight). The animals treated with the extract showed significant improvement in healing when compared with the control untreated animals. The indicators of the wound healing such as collagen-hydroxyproline and hexosamine contents were significantly increased in the treated group indicating accelerated wound healing in the treated animals. The acute phase proteins—haptoglobin and orosomucoid which were increased due to burn injury were found to be decreased significantly in 200 mg/kg body weight extract treated animals. The antioxidant defense mechanism, which was decreased in the liver during burn injury, was found to be enhanced in treated animals. The lipid peroxidation was significantly lowered in the treated group when compared to control animals. Tissue damage marker enzymes- alkaline phosphatase, alanine and aspartate transaminases were significantly lowered in the treated groups in a dose dependant manner. The histopathological analyses of skin tissue also give the evidence of the increased healing potential of the extract after burn injury. PMID:18818737

  5. Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.

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    Amir Hossein Esmaeili

    2014-06-01

    Full Text Available Objectives: Eriobotrya japonica belongs to the Rosaceae. Studies have shown that the flowers of this plant are rich in phenolic and flavonoid compounds. Accorrdingly, the evaluation of antioxidative effects of Eriobotrya japonica Flower Extract (EJFE have been performed in vitro. Material and Methods: In this study, to investigate the influences of components of EJFE on its antioxidative activity, extract was prepared using hydro-alcoholic (25:75 V/V solvent and the antioxidative activity of the extract was evaluated based on the scavenging of various radicals (DPPH and H2O2 by spectrophotometric method and chelating of ferrous ions by ferrozine reagent. Results: HPLC analysis of the Eriobotrya japonica Flower Extract (EJFE revealed hesperetin and gallic acid as the major antioxidants. When the content of total flavonoid and polyphenolic compounds in the flower extract of this plant was examined, a significantly higher level of total polyphenols was found in Eriobotrya japonica flower extract. Conclusion: Results demonstrate that the high ability to scavenge free radicals, reducing power, and Fe+2chelating activity exerted by the EJFE were due to the high content of hesperetin and gallic acid in the flowers.

  6. Sequential Combination of Microwave- and Ultrasound-Assisted Extraction of Total Flavonoids from Osmanthus fragrans Lour. Flowers

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    Jianfeng Yu

    2017-12-01

    Full Text Available Microwave-assisted and ultrasound-assisted extraction assays were used to isolate total flavonoids (TF from Osmanthus fragrans flowers. The effects of the solid-liquid ratio, ethanol concentration, microwave power, microwave extraction time, ultrasonic power and ultrasonic extraction time on the yield of TF were studied. A sequential combination of microwave- and ultrasound-assisted extraction (SC-MUAE methods was developed, which was subsequently optimized by Box-Behnken design-response surface methodology (BBD-RSM. The interaction effects of the ethanol concentration (40–60%, microwave extraction time (5–7 min, ultrasonic extraction time (8–12 min and ultrasonic power (210–430 W on the yield of TF were investigated. The optimum operating parameters for the extraction of TF were determined to be as follows: ethanol concentration (48.15%, microwave extraction time (6.43 min, ultrasonic extraction time (10.09 min and ultrasonic power (370.9 W. Under these conditions, the extraction yield of TF was 7.86 mg/g.

  7. Content of nutrient and antinutrient in edible flowers of wild plants in Mexico.

    Science.gov (United States)

    Sotelo, Angela; López-García, Semeí; Basurto-Peña, Francisco

    2007-09-01

    Nutrient and antinutritional/toxic factors present in some edible flowers consumed in Mexico were determined. The edible flowers were: Agave salmiana, Aloe vera, Arbutus xalapensis, Cucurbita pepo (cultivated), Erythrina americana, Erythrina caribaea, Euphorbia radians benth and Yucca filifera. The nutrient content in the flowers studied is similar to that of the edible leaves and flowers studied mainly in Africa. The moisture content of the flowers varied from 860 to 932 g kg(-1). Crude protein (CP) was between 113 to 275 g kg(-1) DM, crude fiber, 104 to 177 g kg(-1) DM and the nitrogen free extract, between 425 to 667 g kg(-1) DM. The highest chemical score (CS) was found in E. americana and A. salmiana; in five samples the limiting amino acid was lysine, and in three of them it was tryptophan. Trypsin inhibitors and hemaglutinnins had a very low concentration. Alkaloids were present in both the Erythrina species and the saponins in A. salmiana and Y. filifera. Cyanogenic glucosides were not found in the studied flowers. The traditional process of preparing these specific flowers before consumption is by cooking them and discarding the broth; in this way the toxic substances are diminished or eliminated. These edible flowers from wild plants consumed in local areas of the country play an important role in the diet of the people at least during the short time of the season where they are blooming.

  8. The Effect of Citrus Aurantium Flowers Aqueous Extract on Sleeping Time and the Level of Anxiety in mice

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    Vahideh Sadat Abbasnia

    2016-11-01

    Full Text Available Background and Aim: In Iranian traditional medicine Citrus Aurantium flower extract is used to treat some neural diseases. Tendency to use medicinal herbs is increasing. The present  study was done to determine the effect of Citrus Aurantium flowers on sleeping time and the level of anxiety in mice. Materials and Methods: In this experimental study, 80 male albino mice (25-30 g were  randomly divided into 8 equal groups. In order to measure . the sleeping time of the subjects . Angel’s method  was applied and the animals were divided into three experimental.groups (200, 400 and 600 mg/kg and one control group. To evaluate their anxiety levels. they were randomly divided into three experimental and one  control group;and for their evaluation plus maze (EPM model was used. The evaluation of anxiety indices included number and percent of time spent in open arm. Different doses of the aqueous extract of Citrus Aurantium flowers (200, 400, 600 mg/kg IP; respectively were intraperitoneally injected into the treated groups.  But, the controls received 10 ml/kg/BW normal saline intraperitoneally in both of the methods. The obtained data was analyzed by means of SPSS . software (V:17 using one-way ANOVA and Tukey’s test(α=0.05. Results: The extract of  Citrus aurantium flowers (200, 400 and 600 mg/kg significantly increased  sleeping time (12/2±0/53, (14/4±037, (15/5±1/22, time-spent of open arm entries (64/4±4/01, (75±3/01, (78±2/01 and arm entries into open arms(5/9±021, (6/6±0/41, (6/8±72 compared to the control group (P<0.05. Conclusion: The current study showed that the aqueous extract of Citrus Aurantium flowers incerases the sleeping time and decreases level of anxity in mice.

  9. Aloe vera: A review of toxicity and adverse clinical effects.

    Science.gov (United States)

    Guo, Xiaoqing; Mei, Nan

    2016-04-02

    The Aloe plant is employed as a dietary supplement in a variety of foods and as an ingredient in cosmetic products. The widespread human exposure and its potential toxic and carcinogenic activities raise safety concerns. Chemical analysis reveals that the Aloe plant contains various polysaccharides and phenolic chemicals, notably anthraquinones. Ingestion of Aloe preparations is associated with diarrhea, hypokalemia, pseudomelanosis coli, kidney failure, as well as phototoxicity and hypersensitive reactions. Recently, Aloe vera whole leaf extract showed clear evidence of carcinogenic activity in rats, and was classified by the International Agency for Research on Cancer as a possible human carcinogen (Group 2B). This review presents updated information on the toxicological effects, including the cytotoxicity, genotoxicity, carcinogenicity, and adverse clinical effects of Aloe vera whole leaf extract, gel, and latex.

  10. Antiplasmodial potential and quantification of aloin and aloe-emodin in Aloe vera collected from different climatic regions of India.

    Science.gov (United States)

    Kumar, Sandeep; Yadav, Manila; Yadav, Amita; Rohilla, Pooja; Yadav, Jaya Parkash

    2017-07-17

    In this study, Aloe vera samples were collected from different climatic regions of India. Quantitative HPTLC (high performance thin layer chromatography) analysis of important anthraquinones aloin and aloe-emodin and antiplasmodial activity of crude aqueous extracts was done to estimate the effects of these constituents on antiplasmodial potential of the plant. HPTLC system equipped with a sample applicator Linomat V with CAMAG sample syringe, twin rough plate development chamber (20 x 10 cm), TLC Scanner 3 and integration software WINCATS 1.4.8 was used for analysis of aloin and aloe-emodin amount. The antiplasmodial activity of plant extracts was assessed against a chloroquine (CQ) sensitive strain of P. falciparum (MRC-2). Minimum Inhibitory Concentration (MIC) of aqueous extracts of selected samples was determined according to the World Health Organization (WHO) recommended method that was based on assessing the inhibition of schizont maturation in a 96-well microtitre plate. EC (effective concentration) values of different samples were observed to predict antiplasmodial potential of the plant in terms of their climatic zones. A maximum quantity of aloin and aloe-emodin i.e. 0.45 and 0.27 mg/g respectively was observed from the 12 samples of Aloe vera. The inhibited parasite growth with EC 50 values ranging from 0.289 to 1056 μg/ml. The antiplasmodial EC 50 value of positive control Chloroquine was observed 0.034 μg/ml and EC 50 values showed by aloin and aloe-emodin was 67 μg/ml and 22 μg/ml respectively. A positive correlation was reported between aloin and aloe-emodin. Antiplasmodial activity was increased with increase in the concentration of aloin and aloe-emodin. The quantity of aloin and aloe-emodin was decreased with rise in temperature hence it was negatively correlated with temperature. The extracts of Aloe vera collected from colder climatic regions showed good antiplasmodial activity and also showed the presence of higher amount of aloin and

  11. Effects of different nitrogen levels and plant density on flower, essential oils and extract production and nitrogen use efficiency of Marigold (Calendula officinalis.

    Directory of Open Access Journals (Sweden)

    ali akbar ameri

    2009-06-01

    Full Text Available Efficient use of nitrogen for medicinal plants production, might increase flower dry matter, essential oil and extract yield and reduce cost of yield production. A two year (2005 and 2006 field study was conducted in Torogh region(36,10° N,59.33° E and 1300 m altitude of Mashhad, Iran, to observe the effects of different nitrogen and densities on flower dry matter, essential oil and extract production and nitrogen use efficiency (NUE in a multi-harvested Marigold (Calendula officinalis. The levels of Nitrogen fertilizer (N were 0, 50, 100 and 150 kg ha-1 and levels of density were 20, 40, 60 and 80 plant m-2. The combined analysis results revealed significant effects of N and density levels on flower dry matter, essential oil and extract production and NUE of Marigold. The highest dry flower production obtained by 150 kg ha-1 N and 80 plant m-2 plant population (102.86 g m-2. The higher flower dry matter production caused more essential oil and extract production in high nitrogen and density levels. Agronomic N-use efficiency (kg flower dry matter yield per kg N applied, physiological efficiency (kg flower dry matter yield per kg N absorbed and fertilizer N-recovery efficiency (kg N absorbed per kg N applied, expressed as % for marigold across treatments ranged from 6.8 to14.9, 12.3 to 33.6 and 55.5 to 77.6, respectively and all were greater for N application at 50 compared with150 kg N ha-1, and under high density than low density. The amount of essential oil and extract per 100g flower dry matter decreased during the flower harvesting period. The higher amount of essential oil and extract obtained at early flowering season. The essential oil and extract ranged from 0.22 to 0.12 (ml. per 100g flower dry matter and 2.74 to 2.13 (g per 100g flower dry matter respectively.

  12. Effect of Mentha pulegium extract and 8-hydroxy quinoline sulphate to extend the quality and vase life of rose (Rosa hybrid) cut flower.

    Science.gov (United States)

    Hashemabadi, Davood; Torkashvand, Ali Mohammadi; Kaviani, Behzad; Bagherzadeh, Maryam; Rezaalipour, Mohaddeseh; Zarchini, Mohammad

    2015-01-01

    Rose is an ornamental plant which contains one of the world's top cut flowers. Vase life of cut rose flower is short. Extracts of Mentha pulegium and 8-hydroxy quinoline sulphate (8-HQS) were used as two preservative solutions, aiming to extend the vase life of cut rose (Rosa hybrid L.) flowers. Rose flowers were treated with a vase solution containing the extract of M. pulegium, at concentrations of 0, 10, 20 and 30%, in combination with 8-HQS at concentrations of 0, 200, 400 and 600 mg l(-1). Longevity of cut roses flowers was determined on the basis of wilting and chlorophyll retention. Cut roses flowers were kept at room temperature (20 ± 2 degrees C) under normal day light and natural ventilation. The vase life of cut flowers studied was prolonged by all 8-HQS and extract treatments. The best concentration of 8-HQS and extractwere 400 mg l(-1) and 10%, respectively. Our results indicated that the flowers treated with the extract and 8-HQS had longer vase life, higher rate of solution uptake and lower SPAD value (total chlorophyll) compared to the control. Also, cut flowers treated with the extract and 8-HQS had least bacterial colonies. The greatest longevity of vase life by 11.20 and 10.25 days was related to 400 mg I(-1) 8-HQS and 10% of extract, respectively. These treatments improved cut vase life more than the control treatment. The maximum solution uptake (1.85 ml g(-1) f.wt.) and minimum SPAD value (2.19) were calculated in 30% extract along with 200 mg l(-1) 8-HQS, and 200 mg l(-1) 8-HQS, respectively. The lowest number of bacterial colonies (55.75) was obtained in treatment of 600 mg l(-1) 8-HQS. Flower quality of specimens treated with extract and 8-HQS was better than those of the control. The experiments were repeated three times with three replicates and a completely randomized design had been used. The present study concludes that it would be possible to use preservative solutions containing extract of M. pulegium L. and 8-HQS to extend vase

  13. Antihyperglycemic activity of Woodfordia fruticosa (Kurz) flowers extracts in glucose metabolism and lipid peroxidation in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Verma, Neeraj; Amresh, G; Sahu, P K; Rao, Ch V; Singh, Anil Pratap

    2012-05-01

    The ethanolic extract of W. fruticosa flowers (250 and 500 mg/kg) significantly reduced fasting blood glucose level and increased insulin level after 21 days treatment in streptozotocin diabetic rats. The extract also increased catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase activities significantly and reduced lipid peroxidation. Glycolytic enzymes showed a significant increase in their levels while a significant decrease was observed in the levels of the gluconeogenic enzymes in ethanolic extract treated diabetic rats. The extract has a favourable effect on the histopathological changes of the pancreatic beta-cells in streptozotocin induced diabetic rats. The results suggest that W. fruticosa possess potential antihyperglycemic effect by regulating glucose homeostasis and antioxidant efficacy in streptozotocin-induced diabetic rats.

  14. Chemical composition of essential oil and hexane extract and antioxidant activity of various extracts of Acmella uliginosa (Sw. Cass flowers from Indonesia

    Directory of Open Access Journals (Sweden)

    Askal Maimulyanti

    2016-07-01

    Full Text Available Medicinal plants are rich sources of natural antioxidant which are used in the prevention and treatment of disease like artherosclerosis, heart stroke, diabetes and cancer and to delay the process of aging. Acmella uliginosa (Sw. Cass is an edible herb traditionally used in the treatment of many diseases. Analysis of volatile components in the flower extract used gas chromatography-mass spectrometry. The results showed the main components of the essential oil were caryophyllene (21.27%, caryophyllene oxide (15.49%, and 3-carene (10.73%. The main components of the hexane extract were N-isobutyl-2E,6Z,8E-decatrienamide (37.80%, α-pinene (4.98% and hexadacanoic acid-methyl ester (4.78%. The antioxidant activity of A. uliginosa (Sw. Cass flower from Indonesia was determined using 1,1, diphenyl-2-picryl hydrazine (DPPH free radical scavenging assay. The IC50 (defined as the total antioxidant necessary to decrease the initial DPPH radical by 50% of extracts was calculated. A comparative study determined that A. uliginosa (Sw. Cass in methanol extract showed higher antioxidant potential (IC50 = 96.83 μg/mL compared to ethyl acetate extract (IC50 = 123.46 μg/mL and n-hexane extract (905.92 μg/mL against DPPH free radicals.

  15. Anticholinesterase activity and chemical profile of an active chromatographic fraction of ethanolic extract from Bellis perennis L. (Asteraceae) flowers

    International Nuclear Information System (INIS)

    Marques, Thiago Henrique Costa; Santos, Pauline Sousa dos; Freitas, Rivelilson Mendes de; Carvalho, Rusbene Bruno Fonseca de; Melo, Cassio Herbert Santos de; David, Juceni Pereira; David, Jorge Mauricio; Lima, Luciano Silva

    2013-01-01

    This work describes the isolation of an active flavonoid fraction and identification of isorhamnetin 3-O-β-D-(6’’-acetyl)- alactopyranoside from flowers of B. perennis, and also the evaluation of anticholinesterase (AChE) activity of ethanolic extract from flowers (EEF) and the active fraction. The chemical structure of the flavonoid was defined on the basis of spectroscopic 1 H NMR, IR and UV data. EEF or flavonoid reduces AChE activity in vivo, while flavonoid also reduces AChE activity in vitro, showing a value of 1.49 μM for 50% inhibitory concentration (IC 50 ), suggesting potential use as an insecticide or in the treatment of neurodegenerative diseases such as Alzheimer’s disease. (author)

  16. Toxicology of the aqueous extract from the flowers of Butea monosperma Lam. and it's metabolomics in yeast cells.

    Science.gov (United States)

    Khan, Washim; Gupta, Shreesh; Ahmad, Sayeed

    2017-10-01

    Due to lack of scientific evidence for the safety of Butea monosperma (Fabaceae), our study aimed to carry out its toxicological profile and to identify its metabolic pattern in yeast cell. The effect of aqueous extract of B. monosperma flower on glucose uptake in yeast cell was evaluated through optimizing pH, temperature, incubation time, substrate concentration and kinetic parameters. Further, the metabolic pattern of extract as such and in yeast cell were analyzed by gas chromatography-mass spectrometry. Mice were administered aqueous extract up to 6000 and 4000 mg/kg for acute oral and intraperitoneal toxicity, respectively, while up to 4500 mg/kg for sub-acute oral toxicity (30 days). Elongation in the lag and log phase was observed in yeast cells supplemented with extract as compared to control. A maximum of 184.9% glucose uptake was observed whereas kinetic parameters (K m and V max ) were 1.38 and 41.91 mol/s, respectively. Out of 75 metabolites found in the extract, 14 and 18 metabolites were utilized by yeast cell after 15 and 30 min of incubation, respectively. The LD 50 of extract administered through intraperitoneal route was estimated to be 3500 mg/kg. The extract did not elicit any significant difference (P ≥ 0.05) in weight gain, food consumption, water intake, hematological, biochemical parameters and histological changes as compared to the normal control. Results ascertained the safety of B. monosperma flower extract which can be explored as potential candidates for the development of anti-diabetic phytopharmaceuticals. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Comparative evaluation of the antimicrobial activity of natural extracts of Morinda citrifolia, papain and aloe vera (all in gel formulation), 2% chlorhexidine gel and calcium hydroxide, against Enterococcus faecalis: An in vitro study

    Science.gov (United States)

    Bhardwaj, Anuj; Ballal, Suma; Velmurugan, Natanasabapathy

    2012-01-01

    Aim: A comparative evaluation of the antimicrobial activity of natural extracts of Morinda citrifolia, papain, and aloe vera (all in gel formulations), 2% chlorhexidine gel and calcium hydroxide, against Enterococcus faecalis—an in vitro study. Materials and Methods: The antimicrobial efficacy was assessed in vitro using dentin shavings collected at 2 depths of 200 and 400 μm. The total colony forming units at the end of 1, 3, and 5 days were assessed. Results: The overall percentage inhibition of bacterial growth (200 and 400 μm depth) was 100% with chlorhexidine gel. This was followed by M. citrifolia gel (86.02%), which showed better antimicrobial efficacy as compared with aloe vera gel (78.9%), papain gel (67.3%), and calcium hydroxide (64.3%). There was no statistical difference between data at 200 and 400 μm depth. Conclusion: Chlorhexidine gel showed the maximum antimicrobial activity against E. faecalis, whereas calcium hydroxide showed the least. Among the natural intracanal medicaments, M. citrifolia gel consistently exhibited good inhibition up to the 5th day followed by aloe vera gel and papain gel. PMID:22876022

  18. Optimisation of supercritical carbon dioxide extraction of essential oil of flowers of tea (Camellia sinensis L.) plants and its antioxidative activity.

    Science.gov (United States)

    Chen, Zhenchun; Mei, Xin; Jin, Yuxia; Kim, Eun-Hye; Yang, Ziyin; Tu, Youying

    2014-01-30

    To extract natural volatile compounds from tea (Camellia sinensis) flowers without thermal degradation and residue of organic solvents, supercritical fluid extraction (SFE) using carbon dioxide was employed to prepare essential oil of tea flowers in the present study. Four important parameters--pressure, temperature, static extraction time, and dynamic extraction time--were selected as independent variables in the SFE. The optimum extraction conditions were the pressure of 30 MPa, temperature of 50°C, static time of 10 min, and dynamic time of 90 min. Based on gas chromatography-mass spectrometry analysis, 59 compounds, including alkanes (45.4%), esters (10.5%), ketones (7.1%), aldehydes (3.7%), terpenes (3.7%), acids (2.1%), alcohols (1.6%), ethers (1.3%) and others (10.3%) were identified in the essential oil of tea flowers. Moreover, the essential oil of tea flowers showed relatively stronger DPPH radical scavenging activity than essential oils of geranium and peppermint, although its antioxidative activity was weaker than those of essential oil of clove, ascorbic acid, tert-butylhydroquinone, and butylated hydroxyanisole. Essential oil of tea flowers using SFE contained many types of volatile compounds and showed considerable DPPH scavenging activity. The information will contribute to the future application of tea flowers as raw materials in health-care food and food flavour industries. © 2013 Society of Chemical Industry.

  19. In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel.

    Science.gov (United States)

    Kang, Min-Cheol; Kim, Seo Young; Kim, Yoon Taek; Kim, Eun-A; Lee, Seung-Hong; Ko, Seok-Chun; Wijesinghe, W A J P; Samarakoon, Kalpa W; Kim, Young-Sun; Cho, Jin Hun; Jang, Hyeang-Su; Jeon, You-Jin

    2014-01-01

    The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. A simple aloe vera plant-extracted microwave and conventional combustion synthesis: Morphological, optical, magnetic and catalytic properties of CoFe2O4 nanostructures

    Science.gov (United States)

    Manikandan, A.; Sridhar, R.; Arul Antony, S.; Ramakrishna, Seeram

    2014-11-01

    Nanocrystalline magnetic spinel CoFe2O4 was synthesized by a simple microwave combustion method (MCM) using ferric nitrate, cobalt nitrate and Aloe vera plant extracted solution. For the comparative study, it was also prepared by a conventional combustion method (CCM). Powder X-ray diffraction, energy dispersive X-ray and selected-area electron diffraction results indicate that the as-synthesized samples have only single-phase spinel structure with high crystallinity and without the presence of other phase impurities. The crystal structure and morphology of the powders were revealed by high resolution scanning electron microscopy and transmission electron microscopy, show that the MCM products of CoFe2O4 samples contain sphere-like nanoparticles (SNPs), whereas the CCM method of samples consist of flake-like nanoplatelets (FNPs). The band gap of the samples was determined by UV-Visible diffuse reflectance and photoluminescence spectroscopy. The magnetization (Ms) results showed a ferromagnetic behavior of the CoFe2O4 nanostructures. The Ms value of CoFe2O4-SNPs is higher i.e. 77.62 emu/g than CoFe2O4-FNPs (25.46 emu/g). The higher Ms value of the sample suggest that the MCM technique is suitable for preparing high quality nanostructures for magnetic applications. Both the samples were successfully tested as catalysts for the conversion of benzyl alcohol. The resulting spinel ferrites were highly selective for the oxidation of benzyl alcohol and exhibit important difference among their activities. It was found that CoFe2O4-SNPs catalyst show the best performance, whereby 99.5% selectivity of benzaldehyde was achieved at close to 93.2% conversion.

  1. Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines

    Directory of Open Access Journals (Sweden)

    Mohamad Ali Geryani

    2016-06-01

    Full Text Available Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI staining of DNA fragmentation by flow cytometry (sub-G1 peak. Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 µg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 µg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction. Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death.

  2. Mosquitocidal and antibacterial activity of green-synthesized silver nanoparticles from Aloe vera extracts: towards an effective tool against the malaria vector Anopheles stephensi?

    Science.gov (United States)

    Dinesh, Devakumar; Murugan, Kadarkarai; Madhiyazhagan, Pari; Panneerselvam, Chellasamy; Kumar, Palanisamy Mahesh; Nicoletti, Marcello; Jiang, Wei; Benelli, Giovanni; Chandramohan, Balamurugan; Suresh, Udaiyan

    2015-04-01

    Mosquitoes represent an important threat for lives of millions of people worldwide, acting as vectors for devastating pathogens, such as malaria, yellow fever, dengue, and West Nile. In addition, pathogens and parasites polluting water also constitute a severe plague for populations of developing countries. Here, we investigated the mosquitocidal and antibacterial properties of Aloe vera leaf extract and silver nanoparticles synthesized using A. vera extract. Mosquitocidal properties were assessed in laboratory against larvae (I-IV instar) and pupae of the malaria vector Anopheles stephensi. Green-synthesized silver nanoparticles were tested against An. stephensi also in field conditions. Antibacterial properties of nanoparticles were evaluated against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi using the agar disk diffusion and minimum inhibitory concentration protocol. The synthesized silver nanoparticles were characterized by UV-vis spectrum, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). In laboratory conditions, the A. vera extract was toxic against An. stephensi larvae and pupae, even at low dosages. LC50 were 48.79 ppm (I instar), 59.09 ppm (II instar), 70.88 ppm (III instar), 83.58 ppm (IV instar), and 152.55 ppm (pupae). Green-synthesized silver nanoparticles were highly toxic against An. stephensi. LC50 were 3.825 ppm (I instar), 4.119 ppm (II instar), 4.982 ppm (III instar), 5.711 ppm (IV instar), and 6.113 ppm (pupae). In field conditions, the application of A. vera-synthesized silver nanoparticles (10 × LC50) leads to An. stephensi larval reduction of 74.5, 86.6, and 97.7%, after 24, 48, and 72 h, respectively. Nanoparticles also showed antibacterial properties, and the maximum concentration tested (150 mg/L) evoked an inhibition zone wider than 80 mm in all tested bacterium species. This study adds knowledge about the use of green synthesis of nanoparticles in

  3. Antipsoriatic activity of ethanolic extract of Woodfordia fruticosa (L.) Kurz flowers in a novel in vivo screening model.

    Science.gov (United States)

    Srivastava, Amit Kumar; Nagar, Hemant Kumar; Chandel, Harinarayan Singh; Ranawat, Mahendra Singh

    2016-01-01

    The aim of this study was to investigate the antipsoriatic activity of ethanolic extract of Woodfordia fruticosa flowers (EEWF) using a novel in vivo screening model. For induction of psoriasis, 0.1 ml of prepared complete Freund's adjuvant (CFA) and formaldehyde mixture (1:10 ratio) was topically applied for 7 days on the dorsum surface of the skin of Swiss albino mice. Psoriasis severity index (PSI) was evaluated by phenotypic (redness, erythema, and scales) and histological features (epidermal thickness). Therapeutic effect of 0.05% and 0.1% (w/w) ointments of EEWF was evaluated after the induction of psoriasis. Ointments of EEWF flowers were applied once daily for 3 weeks, and antipsoriatic activity was evaluated by scoring the PSI and histological examination. We observed the phenotypic and histological features and found a progressive reduction ( P < 0.05) in the severity of psoriatic lesions (redness, erythema, and scales) from day 7 to 21 st day and decreased epidermal thickness in animals treated with 0.05% and 0.1% (w/w) ointments of EEWF. The results showed that 0.05% and 0.1% (w/w) ointments of EEWF have dose-dependent beneficial effects in CFA and formaldehyde-induced psoriasis. The present investigation revealed that W. fruticosa flowers possess potent antipsoriatic activity and can be used for psoriasis treatment.

  4. Effects of Soursop flowers (Annona muricata L. extract on chemical changes of refined palm olein stored at frying temperature

    Directory of Open Access Journals (Sweden)

    Tonfack Djikeng Fabrice

    2017-12-01

    Full Text Available In this study, the antioxidant activity of soursop flowers extract in delaying palm olein oxidation at frying temperature was assessed. The oil was supplemented with the extract at concentrations 200–1800 ppm, and stored in the oven at 180 °C for 6 days (4 h heating per day. Palm olein containing butylated hydroxytoluene (BHT served as positive control while the same oil without antioxidant (Control served as negative one, in order to monitor changes in oils. After each two heating days, oil samples were collected, and their chemical indexes were determined. Peroxide, para-anisidine, TOTOX, thiobarbituric acid and iodine values were the parameters evaluated. Additionally, the evolution of the fatty acid composition of each oil sample during the storage was assessed by gas-chromatography using a flame ionization detector (GC/FID. Generally, palm olein samples containing the natural extract have exhibited the lowest rate of oxidation, and were efficient in limiting the destruction of unsaturated fatty acids in oil at frying temperature. The order of effectiveness in inhibiting oil oxidation was the following: PO + AnM1800ppm> PO + AnM1400ppm> PO + AnM1000ppm > PO + An.M600ppm> PO + An.M200ppm> PO + BHT200ppm = Control. From these results, it can be concluded that soursop flowers are a potent sources of natural antioxidants for stabilization of palm olein. Keywords: Soursop flowers, Stabilization, Natural antioxidant, Palm olein, Frying, Oxidative stability

  5. Spectrophotometric Determination of Total Sulfite in White Wine Samples Using Crude Extracts from Flowers

    Science.gov (United States)

    Flora Barbosa Soares, Márlon Herbert; Ramos, Luiz Antonio; Tadeu Gomes Cavalheiro, Éder

    2002-09-01

    A didactic spectrophotometric method for determining the sulfite content in white wine samples is proposed. It is based upon a discoloring reaction between flower anthocyanins and the sulfite in basic media. Students' results obtained from iodometric data agreed well with results obtained by the proposed procedure. The use of natural dyes attracted students' interest, enhancing the learning process.

  6. flower extract as an improvised indicator in acid – base titration

    African Journals Online (AJOL)

    USER

    laboratory practical with an instructional material that is useful, economical, simple and accurate for the said titration. Therefore, it is recommended for integrated science and chemistry instructions. Keywords: Flower, acid, base, indicator, titration. INTRODUCTION. In Nigeria, factors militating against science education.

  7. Extraction of Flavonoids from the Flowers of Abelmoschus manihot (L. Medic by Modified Supercritical CO2 Extraction and Determination of Antioxidant and Anti-Adipogenic Activity

    Directory of Open Access Journals (Sweden)

    Jingjing Li

    2016-06-01

    Full Text Available Abelmoschus manihot (L. Medic has been used for many years in Chinese traditional medicine. In this study, supercritical CO2 plus a modifier was utilized to extract flavonoids from the flowers of Abelmoschus manihot (L. Medic. The effects of temperature (40 °C–60 °C, pressure (10–30 MPa and different concentrations of ethanol as modifier (60%–90%, ethanol:water, v/v on major flavonol content and the antioxidant activity of the extracts were studied by response surface methodology (RSM using a Box-Behnken design. The flavonol content was calculated as the sum of the concentrations of seven major flavonoids, namely rutin, hyperin, isoquercetin, hibifolin, myricetin, quercetin-3′-O-glucoside and quercetin, which were simultaneously determined by a HPLC method. The antioxidant activity was evaluated by a 2,2-diphenyl-1-picrylhydarzyl (DPPH free radical-scavenging assay. The results showed that three factors and their interactions could be well fitted to second-order polynomial models (p < 0.05. At the optimal extraction conditions for flavonol content (20 MPa, 52 °C, and 85% ethanol content, the yield of flavonoids was 41.96 mg/g and the IC50 value was 0.288 mg/mL, respectively, suggesting the extract has high antioxidant activity. Furthermore, the anti-adipogenic activity of the extract on the 3T3-L1 cell line was investigated. The results indicated that it can downregulate PPARγ and C/EBPα expression at mRNA. In summary, in this study, we have established a cost-effective method for the extraction of flavonoids from the flowers of Abelmoschus manihot (L. Medic using supercritical fluid extraction and the extracts exhibited potent antioxidant and anti-adipogenic effects, suggesting a possible therapeutic approach for the prevention and treatment of obesity.

  8. Flowers, Beautiful Flowers

    Science.gov (United States)

    School Arts: The Art Education Magazine for Teachers, 2005

    2005-01-01

    In the lesson described, the middle school students had been studying the artist Georgia O'Keeffe and the history of her work. Students enhanced their flower portraits by adding a matching border and connecting the lesson to other subject areas. Students dissected a flower and drew a small diagram of the flower and labeled the parts. This is an…

  9. Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts.

    Science.gov (United States)

    Micucci, Matteo; Malaguti, Marco; Toschi, Tullia Gallina; Di Lecce, Giuseppe; Aldini, Rita; Angeletti, Andrea; Chiarini, Alberto; Budriesi, Roberta; Hrelia, Silvana

    2015-01-01

    This study was aimed at investigating the cardiovascular effects of an Olea europea L. leaf extract (OEE), of a Hibiscus sabdariffa L. flower extract (HSE), and of their 13 : 2 w/w mixture in order to assess their cardiac and vascular activity. Both extracts were fully characterized in their bioactive compounds by HPLC-MS/MS analysis. The study was performed using primary vascular endothelial cells (HUVECs) to investigate the antioxidant and cytoprotective effect of the extracts and their mixture and isolated guinea-pig left and right atria and aorta to evaluate the inotropic and chronotropic activities and vasorelaxant properties. In cultured HUVECs, OEE and HSE reduced intracellular reactive oxygen species formation and improved cell viability, following oxidative stress in dose-dependent manner. OEE and HSE exerted negative inotropic and vasorelaxant effects without any chronotropic property. Interestingly, the mixture exerted higher cytoprotective effects and antioxidant activities. Moreover, the mixture exerted an inotropic effect similar to each single extract, while it revealed an intrinsic negative chronotropic activity different from the single extract; its relaxant activity was higher than that of each single extract. In conclusion OEE and HSE mixture has a good potential for pharmaceutical and nutraceutical application, thanks to the synergistic effects of the single phytochemicals.

  10. Anti-inflammatory activity of flower extract of Calendula officinalis Linn. and its possible mechanism of action.

    Science.gov (United States)

    Preethi, Korengath Chandran; Kuttan, Girija; Kuttan, Ramadasan

    2009-02-01

    Calendula officinalis flower extract possessed significant anti-inflammatory activity against carrageenan and dextran-induced acute paw edema. Oral administration of 250 and 500 mg/kg body weight Calendula extract produced significant inhibition (50.6 and 65.9% respectively) in paw edema of animals induced by carrageenan and 41.9 and 42.4% respectively with inflammation produced by dextran. In chronic anti-inflammatory model using formalin, administration of 250 and 500 mg/kg body weight Calendula extract produced an inhibition of 32.9 and 62.3% respectively compared to controls. TNF-alpha production by macrophage culture treated with lipopolysaccharide (LPS) was found to be significantly inhibited by Calendula extract. Moreover, increased levels of proinflammatory cytokines IL- 1beta, IL-6, TNF-alpha and IFN-gamma and acute phase protein, C- reactive protein (CRP) in mice produced by LPS injection were inhibited significantly by the extract. LPS induced cyclooxygenase-2 (Cox-2) levels in mice spleen were also found to be inhibited by extract treatment. The results showed that potent anti-inflammatory response of C. officinalis extract may be mediated by the inhibition of proinflammatory cytokines and Cox-2 and subsequent prostaglandin synthesis.

  11. Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts

    Directory of Open Access Journals (Sweden)

    Matteo Micucci

    2015-01-01

    Full Text Available This study was aimed at investigating the cardiovascular effects of an Olea europea L. leaf extract (OEE, of a Hibiscus sabdariffa L. flower extract (HSE, and of their 13 : 2 w/w mixture in order to assess their cardiac and vascular activity. Both extracts were fully characterized in their bioactive compounds by HPLC-MS/MS analysis. The study was performed using primary vascular endothelial cells (HUVECs to investigate the antioxidant and cytoprotective effect of the extracts and their mixture and isolated guinea-pig left and right atria and aorta to evaluate the inotropic and chronotropic activities and vasorelaxant properties. In cultured HUVECs, OEE and HSE reduced intracellular reactive oxygen species formation and improved cell viability, following oxidative stress in dose-dependent manner. OEE and HSE exerted negative inotropic and vasorelaxant effects without any chronotropic property. Interestingly, the mixture exerted higher cytoprotective effects and antioxidant activities. Moreover, the mixture exerted an inotropic effect similar to each single extract, while it revealed an intrinsic negative chronotropic activity different from the single extract; its relaxant activity was higher than that of each single extract. In conclusion OEE and HSE mixture has a good potential for pharmaceutical and nutraceutical application, thanks to the synergistic effects of the single phytochemicals.

  12. Cytotoxic Compounds from Aloe megalacantha

    Directory of Open Access Journals (Sweden)

    Negera Abdissa

    2017-07-01

    Full Text Available Phytochemical investigation of the ethyl acetate extract of the roots of Aloe megalacantha led to the isolation of four new natural products—1,8-dimethoxynepodinol (1, aloesaponarin III (2, 10-O-methylchrysalodin (3 and methyl-26-O-feruloyl-oxyhexacosanate (4—along with ten known compounds. All purified metabolites were characterized by NMR, mass spectrometric analyses and comparison with literature data. The isolates were evaluated for their cytotoxic activity against a human cervix carcinoma cell line KB-3-1 and some of them exhibited good activity, with aloesaponarin II (IC50 = 0.98 µM being the most active compound.

  13. ALOS-2 initial results

    Science.gov (United States)

    Kankaku, Yukihiro; Suzuki, Shinichi; Shimada, Masanobu

    2015-10-01

    The Advanced Land Observing Satellite-2 (ALOS-2) was launched from Tanegashima Space Center by H-IIA rocket successfully on 24th May 2014. ALOS-2 carries the Phased Array type L-band Synthetic Aperture Radar-2 (PALSAR-2) as the state-of-the-art L-band SAR system which succeeds to PALSAR onboard ALOS. PALSAR-2 uses almost whole bandwidth allocated for L-band active sensor of Earth Exploration Satellites Service specified by the Radio Regulation in order to realize the high resolution observation, and also, it transmits more than 6 kW power for lower Noise Equivalent Sigma Zero using 180 TRMs driven by Gallium Nitride (GaN) amplifier which is the first use in space. Furthermore, because ALOS-2 carries the SAR system only, PALSAR-2 antenna can be mounted under the satellite body. It enables to observe right-/left-looking observation by satellite maneuvering. And the high accuracy orbit control to maintain the satellite within 500 m radius tube against the reference orbit enables high coherence for the InSAR processing. Using these new technologies, ALOS-2 has been operating to fulfill the mission requirements such as disaster monitoring and so on. This document introduces the initial result of ALOS-2 from the first year operation.

  14. Ultrasound-assisted extraction of natural antioxidants from the flower of Limonium sinuatum: Optimization and comparison with conventional methods.

    Science.gov (United States)

    Xu, Dong-Ping; Zheng, Jie; Zhou, Yue; Li, Ya; Li, Sha; Li, Hua-Bin

    2017-02-15

    Natural antioxidants are widely used as dietary supplements or food additives. An optimized method of ultrasound-assisted extraction (UAE) was proposed for the effective extraction of antioxidants from the flowers of Limonium sinuatum and evaluated by response surface methodology. In this study, ethanol concentration, ratio of solvent to solid, ultrasonication time and temperature were investigated and optimized using a central composite rotatable design. The optimum extraction conditions were as follows: ethanol concentration, 60%; ratio of solvent to solid, 56.9:1mL/g; ultrasonication time, 9.8min; and temperature, 40°C. Under the optimal UAE conditions, the experimental values (483.01±15.39μmolTrolox/gDW) matched with those predicted (494.13μmolTrolox/gDW) within a 95% confidence level. In addition, the antioxidant activities of UAE were compared with those of conventional maceration and Soxhlet extraction methods, and the ultrasound-assisted extraction could give higher yield of antioxidants and markedly reduce the extraction time. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Identification of New Compounds from Sage Flowers (Salvia officinalis L.) as Markers for Quality Control and the Influence of the Manufacturing Technology on the Chemical Composition and Antibacterial Activity of Sage Flower Extracts.

    Science.gov (United States)

    Gericke, Sebastian; Lübken, Tilo; Wolf, Diana; Kaiser, Martin; Hannig, Christian; Speer, Karl

    2018-02-28

    Parts of Salvia species such as its flowers and leaves are currently used as a culinary herb and for some medicinal applications. To distinguish the different sage extracts it is necessary to analyze their individual chemical compositions. Their characteristic compounds might be established as markers to differentiate between sage flowers and leaf extracts or to determine the manufacturing technology and storage conditions. Tri-p-coumaroylspermidine can be detected only in flowers and has been described here for Salvia and Lavandula species for the first time. Markers for oxidation processes are the novel compounds salviquinone A and B, which were generated from carnosol by exposure to oxygen. Caffeic acid ethyl ester was established as an indirect marker for the usage of ethanol as extraction solvent. The compounds were identified by LC-QTOF-HRESIMS, LC-MS, NMR, IR, and single-crystal X-ray diffraction after isolation by semipreparative HPLC. Furthermore, sage flower resin showed interesting antibacterial in vitro activities against Gram-positive and Gram-negative bacteria.

  16. Extracting Features of Acacia Plantation and Natural Forest in the Mountainous Region of Sarawak, Malaysia by ALOS/AVNIR2 Image

    Science.gov (United States)

    Fadaei, H.; Ishii, R.; Suzuki, R.; Kendawang, J.

    2013-12-01

    The remote sensing technique has provided useful information to detect spatio-temporal changes in the land cover of tropical forests. Land cover characteristics derived from satellite image can be applied to the estimation of ecosystem services and biodiversity over an extensive area, and such land cover information would provide valuable information to global and local people to understand the significance of the tropical ecosystem. This study was conducted in the Acacia plantations and natural forest situated in the mountainous region which has different ecological characteristic from that in flat and low land area in Sarawak, Malaysia. The main objective of this study is to compare extract the characteristic of them by analyzing the ALOS/AVNIR2 images and ground truthing obtained by the forest survey. We implemented a ground-based forest survey at Aacia plantations and natural forest in the mountainous region in Sarawak, Malaysia in June, 2013 and acquired the forest structure data (tree height, diameter at breast height (DBH), crown diameter, tree spacing) and spectral reflectance data at the three sample plots of Acacia plantation that has 10 x 10m area. As for the spectral reflectance data, we measured the spectral reflectance of the end members of forest such as leaves, stems, road surface, and forest floor by the spectro-radiometer. Such forest structure and spectral data were incorporated into the image analysis by support vector machine (SVM) and object-base/texture analysis. Consequently, land covers on the AVNIR2 image were classified into three forest types (natural forest, oil palm plantation and acacia mangium plantation), then the characteristic of each category was examined. We additionally used the tree age data of acacia plantation for the classification. A unique feature was found in vegetation spectral reflectance of Acacia plantations. The curve of the spectral reflectance shows two peaks around 0.3μm and 0.6 - 0.8μm that can be assumed to

  17. Effect of extraction technique on the content and antioxidant activity of crude extract of Anacyclus clavatus flowers and their essential oil composition.

    Science.gov (United States)

    Aliboudhar, Hamza; Tigrine-Kordjani, Nacéra

    2014-01-01

    Anacyclus clavatus is a plant used as food and remedy. The objective of this work was to study the effect of extraction technique on the antioxidant property, total phenol and flavonoid contents of crude extracts from A. clavatus flowers and their essential oil composition. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric-reducing power, β-carotene and total antioxidant capacity assays have demonstrated the significant antioxidant ability of different crude extracts obtained by using the following extraction methods: Soxhlet, microwave heating, heat reflux (HRE) and maceration. The activity of the extract obtained by HRE was the highest (112.06 ± 2.89 μg/mL) evaluated by the DPPH assay. Extraction of essential oil was performed by microwave-assisted hydro-distillation (MAHD) and by hydro-distillation (HD). A significant difference was observed in both essential oils, despite the common main family and major constituents, such as artemisia ketone (10.0 ± 0.8% for MAHD vs. 6.5 ± 0.5 for HD) and pinocarvone (4.1 ± 0.4% for MAHD vs. 1.1 ± 0.1% for HD).

  18. The Effect of Aloe vera Extract on Adherence of Candida albicans and Other Properties of Heat Cure Denture Soft Lining Material

    Directory of Open Access Journals (Sweden)

    Aseel Riyadh Abdulwahhab

    2018-03-01

    Full Text Available Objective: One of the serious drawbacks of denture soft lining materials is colonization by Candida albicans that might eventually leads to denture stomatitis. This can be treated either systemically or locally. With the recent increase interest in medicinal plants, this study aimed to evaluate the effect of Aloe vera powder incorporated with heat cure acrylic soft-liner powder on the adherence of Candida albicans, shear bond strength and tear strength. Methods: According to the results of pilot study, two percentages (3% and 10% of aloe vera powder was used. Candida adherence test, shear bond strength and tear strength tests were performed, also the long-term effect was evaluated after 2 and 4 weeks incubation in artificial saliva. All data was analyzed using SPSS software (version 24. Descriptive and inferential statistics, ANOVA test with post-hoc analysis was applied. Results: The results indicated that both concentrations of aloe vera showed a statistically highly significant decrease in Candida albicans cell count in comparison to control group, also a significant increase in shear bond strength and non-significant difference in tear strength of soft liner for the experimental groups. After 2 and 4 weeks incubation in artificial saliva, all experimental groups showed a statistically significant decrease in Candida albicans cell count and a statistically significant increase in shear bond strength and tear strength test. Conclusion: Incorporation of aloe vera powder with heat cure acrylic soft-liner powder helps to add an anti-candidal property to the soft liner, also this addition results in improvement in shear bond strength and tear strength.

  19. Dandelion (Taraxacum officinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro.

    Science.gov (United States)

    Hu, C; Kitts, D D

    2005-08-01

    Flavonoids and coumaric acid derivatives were identified from dandelion flower (Taraxacum officinale). Characteristics of chain-breaking antioxidants, such as extended lag phase and reduced propagation rate, were observed in oxidation of linoleic acid emulsion with the addition of dandelion flower extract (DFE). DFE suppressed both superoxide and hydroxyl radical, while the latter was further distinguished by both site-specific and non-specific hydroxyl radical inhibition. DPPH-radical-scavenging activity and a synergistic effect with alpha-tocopherol were attributed to the reducing activity derived from phenolic content of DFE. A significant (p < 0.05) and concentration-dependent, reduced nitric oxide production from acterial-lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells was observed with the addition of DFE. Moreover, peroxyl-radical-induced intracellular oxidation of RAW264.7 cells was inhibited significantly (p < 0.05) by the addition of DFE over a range of concentrations. These results showed that the DFE possessed marked antioxidant activity in both biological and chemical models. Furthermore, the efficacy of DFE in inhibiting both reactive oxygen species and nitric oxide were attributed to its phenolic content.

  20. Dye sensitized solar cell (DSSC) with natural dyes extracted from Jatropha leaves and purple Chrysanthemum flowers as sensitizer

    Science.gov (United States)

    Tahir, Dahlang; Satriani, Wilda; Gareso, P. L.; Abdullah, B.

    2018-03-01

    DSSC (Dye-Sensitized Solar Cell) prototype has been investigated using Jatropha leaves and purple Chrysanthemum flowers as natural dyes. DSSC consists of working electrode and counter electrode. A working electrode composed of semiconductor nanoparticles TiO2 that has been coated with dye molecules. Dye molecules serve as light photon catchers, while semiconductor nanoparticles TiO2 function to absorb and forward photons into electrons. In the electrode counter given catalyst carbon, serves to accelerate the reaction kinetics of triiodide reduction process on transparent conductive oxide (TCO). DSSC using TiO2 as a semiconductor material and natural dyes as sensitizer from Jatropha leaves and purple Chrysanthemum flowers are successful produced. The physical properties of the working electrode have been determined by using XRD and the chemical properties of the TiO2 powder and dye powder using FTIR and dye solution using UV-Vis. The resulted fabrications are also examined its I-V characteristics. The best performance is generated by mixed dye 1.91 x 10-3 % compared than those DSSC for dye extracted from Jatropha leaves or purple Chrysanthemum. The characterization results show that the higher of the absorption wavelength of the DSSC efficiency is high.

  1. THE POTENTIAL OF EXTRACT OF LEAVES AND FLOWERS OF Lantana camara Linn. AS AN ANTIBACTERIAL FOR CATFISH (Clarias gariepinus INFECTED BY Aeromonas hydrophila

    Directory of Open Access Journals (Sweden)

    Indriyani Nur

    2009-06-01

    Full Text Available Freshwater catfish culture has been hampered by bacterial diseases. One of the agents of the bacterial disease is Motile Aeromonas Septicemia (MAS. The application of synthetic antibiotics has had some disadvantages such as bacterial resistance and undegradable in water. One of the potential antibacterial herbs is Lantana camara. Information of Lantana as an antibacterial on catfish is still limited. Therefore, the experiment of utilization of Lantana as an antibacterial for catfish should be conducted. The experiment was carried out to evaluate the potential of Lantana extract as an antibacterial of A. hydrophila for catfish. The completely randomized design was applied consisting of four treatments using two parts of the plant, leaves and flowers. The treatments were: A = 1,000 ppm of leaves; B = 2,000 ppm of leaves; C = 1,000 ppm of flowers; D = 2,000 ppm of flowers, and control. Lantana extracts were diluted into each culture media which had been infected with A. hydrophila. Several factors were observed in this experiment such as prevalence with of MAS disease, survival rate, percentage of haematocrites and total of leukocytes of fish blood. The results showed that the fish treated with 2,000 ppm of flowers extract had a lower in prevalence of MAS disease and higher in survival rate than those treated with 1,000 ppm; 2,000 ppm of leaves; and 1,000 ppm of flowers, respectively. However, percentage of haematocrytes and total of leucocytes was not influenced by the extracts from different parts of Lantana plant. In conclusion, 2,000 ppm of Lantana flowers extract might be useful as an antibacterial of A. hydrophila for catfish culture.

  2. Phyto-crystallization of silver and gold by Erigeron annuus (L. Pers flower extract and catalytic potential of synthesized and commercial nano silver immobilized on sodium alginate hydrogel

    Directory of Open Access Journals (Sweden)

    Palanivel Velmurugan

    2016-05-01

    Full Text Available A green, eco-friendly approach for the synthesis of silver and gold nanoparticles (AgNPs and AuNPs using Erigeron annuus (L. pers flower extract as both the reducing and capping agent is reported for the first time. Optimal nanoparticle production was achieved by adjusting various parameters including pH, extract concentration, metal ion concentration, and time. Initial verification of AgNP and AuNP production was done by visual observation and measuring surface plasmon spectra at 434 and 537 nm, respectively. The synthesized AgNPs and AuNPs were characterized by high resolution-transmission electron microscopy (HR-TEM, X-ray diffraction (XRD, energy dispersive spectrophotometry (EDS, Fourier transform infrared spectroscopy (FTIR and zeta potential. The catalytic potential of E. annuus flower extract, silver ions, synthesized AgNPs, commercial grade AgNPs, and a mixture of flower extract and AgNPs immobilized on sodium alginate hydrogel beads (Na/Al HB was analyzed. The ability of these immobilized materials to degrade methylene blue was investigated. Commercial grade AgNPs immobilized with Na/Al HB 1.5 g/20 mL were observed to have good catalytic activity followed by a mixture of synthesized AgNPs immobilized with Na/Al HB and E. annuus flower extract immobilized with Na/Al HB at 1.5 g/20 mL.

  3. Anti-diabetic action of Punica granatum flower extract: Activation of PPAR-γ and identification of an active component

    International Nuclear Information System (INIS)

    Huang, Tom H.W.; Peng Gang; Kota, Bhavani P.; Li, George Q.; Yamahara, Johji; Roufogalis, Basil D.; Li Yuhao

    2005-01-01

    Peroxisome proliferator-activated receptor (PPAR)-γ activators are widely used in the treatment of type 2 diabetes because they improve the sensitivity of insulin receptors. Punica granatum flower (PGF) has been used as an anti-diabetic medicine in Unani medicinal literature. The mechanism of actions is, however, unknown. In the current study, we demonstrated that 6-week oral administration of methanol extract from PGF (500 mg/kg, daily) inhibited glucose loading-induced increase of plasma glucose levels in Zucker diabetic fatty rats (ZDF), a genetic animal model for type 2 diabetes, whereas it did not inhibit the increase in Zucker lean rats (ZL). The treatment did not lower the plasma glucose levels in fasted ZDF and ZL rats. Furthermore, RT-PCR results demonstrated that the PGF extract treatment in ZDF rats enhanced cardiac PPAR-γ mRNA expression and restored the down-regulated cardiac glucose transporter (GLUT)-4 (the insulin-dependent isoform of GLUTs) mRNA. These results suggest that the anti-diabetic activity of PGF extract may result from improved sensitivity of the insulin receptor. From the in vitro studies, we demonstrated that the PGF extract enhanced PPAR-γ mRNA and protein expression and increased PPAR-γ-dependent mRNA expression and activity of lipoprotein lipase in human THP-1-differentiated macrophage cells. Phytochemical investigation demonstrated that gallic acid in PGF extract is mostly responsible for this activity. Thus, our findings indicate that PPAR-γ is a molecular target for PGF extract and its prominent component gallic acid, and provide a better understanding of the potential mechanism of the anti-diabetic action of PGF

  4. [Relationship between antibacterial activity of aloe and its anthaquinone compounds].

    Science.gov (United States)

    Tian, Bing; Hua, Yue-jin; Ma, Xiao-qiong; Wang, Guan-lin

    2003-11-01

    To investigate the relationship between the antibacterial activity of aloe and its contents of anthaquinone compounds, measure and compale antibacterial activities of aloin and aloe-emodin, and analyse the effect of glycoside on the antibacterial activity of aloin. The antibacterial activities of the extracts from the outer leaf of Aloe saponaria Haw, aloin and aloe-emodin against three Gram-negative and two Gram-positive bacteria were investigated with the method of agar diffusion. The antibacterial effect of aloin on E. coli was further studied with scanning electron microscopy. The antibacterial activities of aloe showed to be dependent on the dose of anthraquinone, aloin (1 g x L(-1)) exhibited higher antibacterial activity [inhibition diameter > (7. 1 +/- 0.15) mm] than Aloe-emodin (inhibition diameter aloin changed the morphology of E. coli and damaged the outer cell structrue. Anthraquinone compounds are the active antibacterial components in aloe and aloin is the main active compound. The glycoside makes it easy for aloin to invade cells and enhances its activity.

  5. Antiplasmodial potential and quantification of aloin and aloe-emodin in Aloe vera collected from different climatic regions of India

    OpenAIRE

    Kumar, Sandeep; Yadav, Manila; Yadav, Amita; Rohilla, Pooja; Yadav, Jaya Parkash

    2017-01-01

    Background In this study, Aloe vera samples were collected from different climatic regions of India. Quantitative HPTLC (high performance thin layer chromatography) analysis of important anthraquinones aloin and aloe-emodin and antiplasmodial activity of crude aqueous extracts was done to estimate the effects of these constituents on antiplasmodial potential of the plant. Methods HPTLC system equipped with a sample applicator Linomat V with CAMAG sample syringe, twin rough plate development c...

  6. Repellency of Plant Extracts against the Legume Flower Thrips Megalurothrips sjostedti (Thysanoptera: Thripidae

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    Andnet Abtew

    2015-06-01

    Full Text Available Megalurothrips sjostedti Trybom is an important pest of cowpea (Vigna unguiculata L. in Africa. To propose an alternative to chemical control, the repellency of 24 plant extracts was evaluated against adult female thrips of M. sjostedti in the laboratory. Plant extracts in ethanol were separately applied on a filter paper disk in a still air visual cue olfactometer. The results showed highly significant differences in repellency among extract type, concentration and their interactions. We classified the level of repellency into four categories as strong, good, moderate and weak or non- repellent based on hierarchical ascendant classification. We identified Piper nigrum, Cinnamomum zeylanicum, Cinnamomum cassia as strong repellents. Five extracts were classified as good, eight as moderate and the remaining eight extracts were weak or non-repellent. Repellency of the extracts increased with the concentration suggesting that the behavioral response of M. sjostedti was dose-dependent. Mono- and sesquiterpene hydrocarbon compounds from seven highly repellent extracts were identified by gas chromatography-mass spectrometry (GC/MS. The use of repellent extracts could be useful in developing integrated pest management strategies for thrips on legume crops. In this regard, the specific modes of action of the identified compounds need to be investigated to incorporate them into the existing crop protection strategies.

  7. EXAFS analysis of cations distribution in structure of Co1−xNixFe2O4 nanoparticles obtained by hydrothermal method in aloe vera extract solution

    International Nuclear Information System (INIS)

    Wongpratat, Unchista; Maensiri, Santi; Swatsitang, Ekaphan

    2016-01-01

    Graphical abstract: It is obvious from the M–H curves at room temperature of Co 1−x Ni x Fe 2 O 4 (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles that partially substitution of the lower Bohr magneton (2 μ B ) and smaller atomic radii (0.55 Å at A site, 0.69 Å at B site) of Ni 2+ ions on the higher Bohr magneton (3 μ B ) and larger atomic radii (0.58 Å at A site, 0.74 Å at B site) of Co 2+ ions can increase the saturation magnetization (M s ) of sample with x = 0.75 to approximately 1.4 times of sample with x = 0, due to the increase of the aspect ratio (surface to volume) of nanoparticles, as a result of particle size decreasing from 37.03 to 12.63 nm. In addition to this, the ferrimagnetic behavior of CoFe 2 O 4 has been changed to superparamagnetic behavior with the dramatic decrease of the coercivity from 1365.60 to 63.15 Oe. - Highlights: • Magnetic behavior of Co 1−x Ni x Fe 2 O 4 NPs depends on Ni content and size of NPs. • Distribution of Co 2+ and Ni 2+ ions in the structure results in the increase of M s . • Superparamagnetic behavior is observed with increasing of the aspect ratio. • M s is increased by a factor 1.4 to a value of 57.57 emu/g in Co 0.25 Ni 0.75 Fe 2 O 4 . • H c is decreased by a factor 20 to a value of 63.15 Oe in Co 0.25 Ni 0.75 Fe 2 O 4 . - Abstract: Effect of cations distribution upon EXAFS analysis on magnetic properties of Co 1−x Ni x Fe 2 O 4 (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles prepared by the hydrothermal method in aloe vera extract solution were studied. XRD analysis confirmed a pure phase of cubic spinel ferrite of all samples. Changes in lattice parameter and particle size depended on the Ni content with partial substitution and site distributions of Co 2+ , Ni 2+ ions of different ionic radii at both tetrahedral and octahedral sites in the crystal structure. Particle sizes of samples estimated by TEM images were found to be in the range of 10.87–62.50 nm. The VSM results at room

  8. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

    OpenAIRE

    Ramachandran, Pushkala; Nagarajan, Srividya

    2014-01-01

    Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG) and papaya. Aloe gel (30%), papaya pulp (15%), spice extract (5%), and citric acid (0.1%) were mixed in given proportion to prepare...

  9. Antioxidant Activity, Antitumor Effect, and Antiaging Property of Proanthocyanidins Extracted from Kunlun Chrysanthemum Flowers

    Directory of Open Access Journals (Sweden)

    Siqun Jing

    2015-01-01

    Full Text Available The objective of the present study was to evaluate the antioxidant activity, antitumor effect, and antiaging property of proanthocyanidins from Kunlun Chrysanthemum flowers (PKCF grown in Xinjiang. In vitro antioxidant experiments results showed that the total antioxidant activity and the scavenging capacity of hydroxyl radicals (•OH and 1,1-diphenyl-2-picrylhydrazyl (DPPH• radicals increased in a concentration-dependent manner and were stronger than those of vitamin C. To investigate the antioxidant activity of PKCF in vivo, we used serum, liver, and kidney from mouse for the measurement of superoxide dismutase (SOD, malondialdehyde (MDA, and total antioxidant capacity (T-AOC. Results indicated that PKCF had antioxidative effect in vivo which significantly improved the activity of SOD and T-AOC and decreased MDA content. To investigate the antitumor activity of PKCF, we used H22 cells, HeLa cells, and Eca-109 cells with Vero cells as control. Inhibition ratio and IC50 values were measured by 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay; PKCF showed great inhibitory activity on H22 cells and HeLa cells. We also used fruit flies as a model for analyzing the anti-aging property of PKCF. Results showed that PKCF has antiaging effect on Drosophila. Results of the present study demonstrated that PKCF could be a promising agent that may find applications in health care, medicine, and cosmetics.

  10. Bioactivity evaluations of ingredients extracted from the flowers of Citrus aurantium L. var. amara Engl.

    Science.gov (United States)

    Zhao, Hai-Yan; Yang, Li; Wei, Jian; Huang, Ming; Jiang, Jian-Guo

    2012-12-15

    Citrus aurantium L. var. amara Engl, a member of genus Citrus (Rutaceae), widespread in China, is used as folk medicine for the treatment of helping digest, phlegm, enteritidis, stomachic and other deceases. In the present research, silica gel column, Sephadex LH-20, mass spectrometer (MS) and nuclear magnetic resonance (NMR) were used to separate and identify the chemical compounds from the flowers of C. aurantium var. amara, and several bioactivity assays were used to evaluate their antioxidant, anti-inflammatory, anti-virus and antitumour activities. Two major compounds, 5-hydroxy-6,7,3',4'-tetramethoxyflavone (HTF) and limonexic acid (LA), were isolated and identified from C. aurantium var. amara for the first time. The results of the bioactivity assays showed that HTF and LA displayed significant antioxidant activities and showed significant inhibition effects on the B16 cell lines at a concentration range from 6.25 to 50 μg/ml, and on the SMCC-7721 cell lines from 12.5 to 200 μg/ml. The antitumour effect, anti-inflammatory activity and the inhibiting expression of HBsAg and HBeAg of 2.2.15 cells displayed the tendency in a concentration-dependent manner. These two compounds from C. aurantium var. amara could potentially be used as a promising natural agent in the pharmaceutical industries. Copyright © 2012 Elsevier Ltd. All rights reserved.

  11. Isolation and Characterization of β-Sitosterol-3-O-β-D-glucoside from the Extract of the Flowers of Viola odorata

    OpenAIRE

    Tania Peshin; H. K. Kar

    2017-01-01

    Isolation from the ethanol extract of the flowers of Viola odorata resulted in the isolation of β-Sitosterol-β-D-glucoside. This compound has not been previously isolated or reported from the flowers of this variety. The isolated β-Sitosterol-3-O-β-D-glucosidewas characterized on the basis of spectroscopic techniques viz. infrared and nuclear magnetic resonance spectroscopy. A tetra acetyl derivative of β-Sitosterol-3-O-β-D-glucoside was synthesized and characterized. On hydrolysis of β-Sitos...

  12. Passion flower extract antagonizes the expression of nicotine locomotor sensitization in rats.

    Science.gov (United States)

    Breivogel, Chris; Jamerson, Brenda

    2012-10-01

    Nicotine, a bioactive component of tobacco, is highly addictive. Numerous therapies have been developed or are currently under investigation for smoking cessation, and all have met with limited success and/or side effects, indicating the need for additional therapies. This study examines the ability of a commerically-available aqueous extract of Passiflora incarnata Linn. (Passifloraceae) to ameliorate the signs of nicotine sensitization using a rat model. Rats were administered 0.4 mg/kg nicotine or vehicle once a day for four consecutive days. Nicotine adminstration produces sensitization of locomotor activity, a phenomenon implicated in the development of nicotine dependence. On the fifth day, locomotor activity of the subjects was monitored as rats from each treatment group were administered 800 mg/kg of Passiflora incarnata extract (or its vehicle) followed by a challenge dose of 0.4 mg/kg nicotine. When given to rats sensitized to nicotine for 4 days, the challenge dose of nicotine increased locomotor activity by more than 2-fold over activity following nicotine challenge in rats treated with vehicle during the sensitization phase. The difference was significant from 15-40 min after nicotine administration. Rats sensitized to nicotine then treated with Passiflora incarnata extract prior to the nicotine challenge exhibited a level of locomotor activity the same as the vehicle-treated controls. Passiflora incarnata extract did antagonize the expression of nicotine locomotor sensitization. Passiflora incarnata extract should be examined in future studies to evaluate its potential for treating nicotine addiction in humans.

  13. Antibacterial Activity of Anthraquinone from Aloe on Spiced Pig Head

    Science.gov (United States)

    Xu, Lingyi; Li, Xiao; Cui, Yuqian; Pang, Meixia; Wang, Fang; Qi, Jinghua

    2017-12-01

    [Objective] To optimize the extraction of anthraquinone from Aloe by ultrasonic extraction and its antibacterialactivity. [Method]The influences of different extraction time and ethanol concentration, on anthraquinone contentwere evaluated by asingle factor experiment. And anthraquinone content was determined by ultraviolet spectrophotometry. The bacteriostasis of anthraquinone on spiced pig head’s common putrefying bacteria: Staphylococcus, Serratieae, Bacillus, Proteus and the minimal inhibitory concentration (MIC) were studied by oxford plate assay system. [Result]The best extraction time was 30 minutes and the best ethanol concentration was 80%. The antibacterial activity of the Aloe anthraquinone on Staphylococcus Aureus, Bacillus Proteus is obviously, the minimum inhibitory concentrations were 0.0625 g/mL, 0.05 g/mL, 0.125 g/mL respectively and no inhibitory effect on Serratieae. [Conclusions] The anthraquinones from Aloe can inhibit a part Of spoilage bacteria inspiced pig heads.

  14. Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract.

    Science.gov (United States)

    Rajakumar, Govindasamy; Gomathi, Thandapani; Thiruvengadam, Muthu; Devi Rajeswari, V; Kalpana, V N; Chung, Ill-Min

    2017-02-01

    The aim of this study is to develop an easy and eco-friendly method for the synthesis of Ag-NPs using extracts from the medicinal plant, Millettia pinnata flower extract and investigate the effects of Ag-NPs on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), antibacterial and cytotoxicity activity. UV-Vis peak at 438 nm confirmed the Ag-NPs absorbance. The SEM analysis results confirmed the presence of spherical shaped Ag-NPs by a huge disparity in the particle size distribution with an average size of 49 ± 0.9 nm. TEM images revealed the formation of Ag-NPs with spherical shape and sizes in the range between 16 and 38 nm. The Ag-NPs showed an excellent inhibitory efficacy against AChE and BChE. The highest antibacterial activity was found against Escherichia coli (20.25 ± 0.91 mm). These nanoparticles showed the cytotoxic effects against brine shrimp (artemia saliana) nauplii with a LD 50 value of 33.92. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Chamomile flower extract-directed CuO nanoparticle formation for its antioxidant and DNA cleavage properties.

    Science.gov (United States)

    Duman, Fatih; Ocsoy, Ismail; Kup, Fatma Ozturk

    2016-03-01

    In this study, we report the synthesis of copper oxide nanoparticles (CuO NPs) using a medicinal plant (Matricaria chamomilla) flower extract as both reducing and capping agent and investigate their antioxidant activity and interaction with plasmid DNA (pBR322).The CuO NPs were characterized using Uv-Vis spectroscopy, FT-IR (Fourier transform infrared spectroscopy), DLS (dynamic light scattering), XRD (X-ray diffraction), EDX (energy-dispersive X-ray) spectroscopy and SEM (scanning electron microscopy). The CuO NPs exhibited nearly mono-distributed and spherical shapes with diameters of 140 nm size. UV-Vis absorption spectrum of CuO NPs gave a broad peak around 285 and 320 nm. The existence of functional groups on the surface of CuO NPs was characterized with FT-IR analysis. XRD pattern showed that the NPs are in the form of a face-centered cubic crystal. Zeta potential value was measured as -20 mV due to the presence of negatively charged functional groups in plant extract. Additionally, we demonstrated concentration-dependent antioxidant activity of CuO NPs and their interaction with plasmid DNA. We assumed that the CuO NPs both cleave and break DNA double helix structure. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

    Directory of Open Access Journals (Sweden)

    Po-Jung Tsai

    2017-01-01

    Full Text Available The excess influx of free fatty acids (FFAs into nonadipose tissues, such as those of liver and kidney, induces lipotoxicity leading to hepatic steatosis and renal dysfunction. The aim of this study was to investigate the protective effects of methanolic flower extracts of Osmanthus fragrans (OF and Chrysanthemum morifolium (CM against FFA-induced lipotoxicity in hepatocytes (human HepG2 cells and renal glomerular mesangial cells (mouse SV40-Mes13 cells. The results showed that OF and CM significantly suppressed FFA-induced intracellular triacylglycerol accumulation via partially inhibiting the gene expression of sterol regulatory element-binding protein-1c (SREBP-1c and glycerol-3-phosphate acyltransferase (GPAT in HepG2 cells. Both extracts inhibited reactive oxygen species (ROS generation by FFA-stimulated HepG2 cells. OF and CM also suppressed the mRNA expression of interleukin- (IL- 1β, IL-6, IL-8, tumor necrosis factor- (TNF- α, and transforming growth factor- (TGF- β by HepG2 cells treated with conditioned medium derived from lipopolysaccharide-treated THP-1 monocytes. Furthermore, OF and CM effectively inhibited oleate-induced cellular lipid accumulation, TGF-β secretion, and overexpression of fibronectin in mesangial cells. In conclusion, OF and CM possess hepatoprotective activity by inhibiting hepatic fat load and inflammation and renal protection by preventing FFA-induced mesangial extracellular matrix formation.

  17. Investigation of Antibacterial and Antioxidant Activities of Alcoholic Extracts of Flower and Root of Dendrostellera Lesserti on Some Human Pathogenic Bacteria

    Directory of Open Access Journals (Sweden)

    M. Alamhulu

    2015-01-01

    Full Text Available Introduction & Objective: With increasing the information about the dangerous side effects of synthetic antibiotics , the demand for natural alternative of these drugs has increased. The purpose of this study is to investigate the antibacterial and antioxidant properties of root and flower extracts of the medicinal plant of Dendrostellera lesserti against some human pathogenic bacteria. Materials & Methods: In this experimental study, Dendrostellera lesserti was collected from Hamadan province in 2013. After identification, the extracts were prepared by maceration method. Antibacterial activities were determined by the agar well diffusion method, MIC (serial dilution method and MBC. Antioxidant properties by DPPH method and amount of phenolic and flavonoid were measured by Folin-ciocalteu and aluminum chloride methods , respectively. The data were analyzed using sas software version 9.2 (P<0.05. Results: The largest growth inhibition zone with diameter of 21.33±.66 mm was seen in Salmonella typhi culture against root methanolic extract. MIC and MBC of root extract was lower in comparison with flower. Methanolic extract of flower in at concentration of 0.8 mg/ml had the highest scavenging percentage of free radical. The higher amount of phenol and flavonoid was related to methanol extract of root, 111.8±2.69 mgGAE/g and 2.25±0.35 mgQ/g, respectively Conclusion: According to the obtained results, the root and flower methanolic extracts of Dendrostellera lesserti contain compounds with antibacterial and antioxidant properties. (Sci J Hamadan Univ Med Sci 2015; 21(4:277-285

  18. Effects of Flower and Fruit Extracts of Melastoma malabathricum Linn. on Growth of Pathogenic Bacteria: Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium

    Directory of Open Access Journals (Sweden)

    Siti Nurhadis Che Omar

    2013-01-01

    Full Text Available Melastoma malabathricum Linn. is a shrub that comes with beautiful pink or purple flowers and has berries-like fruits rich in anthocyanins. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against Listeria monocytogenes IMR L55, Staphylococcus aureus IMR S244, Escherichia coli IMR E30, and Salmonella typhimurium IMR S100 using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their minimum inhibitory concentrations (MICs and minimum bactericidal concentrations (MBCs. In addition, the growth of Listeria monocytogenes IMR L55 and Staphylococcus aureus IMR S244 grown in medium supplemented with the respective extracts at different temperatures (4°C, 25°C, and 37°C and pHs (4, 6, 7, and 8 was determined.

  19. Anti-diabetic effects of hydroalcohlic juglans regia male flower extract on blood glucose level and on liver enzymes activity in intact and diabetogenized adult male rat

    Directory of Open Access Journals (Sweden)

    Seyyed Ebrahim Hosseini

    2012-08-01

    Full Text Available Background and Aim: Diabetes is a metabolic disorder resulting from defects in insulin secretion or function. Walnut is a nutrient used in traditional medicine to treat diabetes. In the current study, anti-diabetic effects of the Hydroalcoholic extract of walnut male flowers on diabetogenized rats by using Streptozocin were evaluated.   Materials and Methods: Seventy two adult male Wistar rats weighing 200-225 g each were randomly selected and divided into three main groups, i.e. control, diabetic, and non-diabetic(intact The control group included 8 rats (n=8. The diabetic and non-diabetic groups covered 32 rats each. Each of these groups were divided into four 8 rats including the control, diabetic, experimental 1, 2, and 3 which received 2, 4, or 6 g/kg of the extract per day for 15 days ,respectively. The three diabetic groups were each treated with the above doses of the extract, and the fourth group received no treatment. Diabetes was induced in diabetic rats through intraperitoneal injection of 60 mg/kg of Streptozotocin. At the end, blood samples were taken from the experimental and control groups and the serum levels of insulin and glucose were measured.   Results: A significant reduction in blood sugar and increase of insulin in diabetics receiving Hydroalcoholic extract of male flowers walnut was observed compared with non-diabetic ones.   Conclusion: Hydroalcoholic extract of male Walnut flowers, due to increasing insulin, causes reduction of blood sugar.

  20. The Genus Aloe: Phytochemistry and Therapeutic Uses Including Treatments for Gastrointestinal Conditions and Chronic Inflammation.

    Science.gov (United States)

    Cock, I E

    2015-01-01

    Plants of the genus Aloe have perhaps the longest recorded history of medicinal usage and are amongst the most widely used plants for traditional medicinal purposes worldwide. Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, but many other species are also used for their therapeutic properties. The Aloes have been used since ancient times, particularly for the treatment of microbial infections, gastrointestinal disorders and inflammatory conditions. In addition to their myriad uses in traditional therapeutics, the Aloes have also been used as components of cosmetic formulations, and in the food and beverage industries. Despite their wide acceptance, studies from different laboratories often report wide variations in the therapeutic bioactivities from within the same Aloe species, even when the same extraction procedures are used. Furthermore, leaves from individual Aloe plants within the same species may have widely varying levels of the bioactive phytochemicals. Phytochemical analyses have shown that many Aloe species contain various carbohydrate polymers (notably glucomannans) and a range of other low molecular weight phenolic compounds including alkaloids, anthraquinones, anthrones, benzene and furan derivatives, chromones, coumarins, flavonoids, phytosterols, pyrans and pyrones. There has been a wealth of information published about the phytochemistry and therapeutic potential of the Aloes (especially Aloe vera). Much of this has been contradictory. Intra- and interspecies differences in the redox state of the individual Aloe components and in the ratios of these components may occur between individual plants. These factors may all affect the physiological properties of Aloe extracts. Due to the structure and chemical nature of many of the Aloe phytochemicals, it is likely that many of the reported medicinal properties are due to antioxidant or prooxidant effects. The antioxidant/prooxidant activities of many Aloe

  1. Antimelanogenic effects of Inula britannica flower petal extract fermented by Lactobacillus plantarum KCCM 11613P*

    Science.gov (United States)

    Park, Eun-hye; Bae, Won-young; Kim, Jae-yeon; Kim, Kee-tae; Paik, Hyun-dong

    2017-01-01

    The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening activity, and the contents of total flavonoids and polyphenolics as bioactive compounds were determined. The skin whitening activity using in vitro or ex vivo tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) staining was examined. The total flavonoid content (TFC) was increased by 13.4% after 72 h-fermentation. The viabilities of the B16F10 cells treated with the fermented and non-fermented control extracts were 100.26% and 92.15% at 500 µg/ml, respectively. In addition, the inhibition of tyrosinase activity was increased by the fermented samples from 29.33% to 41.74% following fermentation for up to 72 h. The tyrosinase activity of the untreated control group was increased to 145.69% in B16F10 cells. The results showed that I. britannica fermented by L. plantarum dose-dependently inhibited tyrosinase activity, which was stimulated by α-melanocyte stimulating hormone. These results suggest that lactic fermented I. britannica extracts can be used as effective skin-whitening materials.

  2. Inhibition of metastasis of B16F-10 melanoma cells in C57BL/6 mice by an extract of Calendula officinalis L flowers.

    Science.gov (United States)

    Preethi, Korangath C; Siveen, Kodappully S; Kuttan, Ramadasan; Kuttan, Girija

    2010-01-01

    To determine the effect of a Calendula officinalis flower extract on lung metastasis by B16F-10 melanoma cells in C57BL/6 mice. Male mice were injected with B16F-10 melanoma cells through the tail vein and simultaneously treated with C.officinalis flower extract. Parameters studied were lung tumor nodule count, life span of animals, gamma glutamyl transpeptidase activity, sialic acid, TNF-α, IL-1β, IL-6, IL-2, GM-CSF, VEGF and TIMP-1 levels in serum, and lung hydroxyproline, uronic acid and hexosamine levels, as well as histopathological features. Effects of C.officinalis on the expression of various genes involved in metastasis like matrix metalloproteases (MMPs), tissue inhibitor of metalloproteases (TIMPs), prolyl hydoxylase, lysyl oxidase, nm23, and proinflammatory cytokines were also investigated. Simultaneous administration of C. officinalis extract to tumor bearing C57BL/6 mice reduced the lung tumor nodules by 74% with 43.3% increase in life span. Elevated levels of hydroxyproline, uronic acid, hexosamine, serum sialic acid and γ-glutamyl transpeptidase in the metastatic controls were found to be significantly lowered in the C. officinalis treated animals. The extract also inhibited expression of MMP-2, MMP-9, prolyl hydroxylase and lysyl oxidase and activated TIMP-1 and TIMP-2 and downregulated proinflammatory cytokines. The present investigation indicated antimetastatic effects of Calendula officinalis flowers through the inhibition of key enzymes involved in processes of metastasis.

  3. Evaluation of Antiulcer and Cytotoxic Potential of the Leaf, Flower, and Fruit Extracts of Calotropis procera and Isolation of a New Lignan Glycoside

    Directory of Open Access Journals (Sweden)

    Areej Mohammad Al-Taweel

    2017-01-01

    Full Text Available Calotropis procera is traditionally used for treating many diseases including ulcers and tumors. It was thus deemed of interest to investigate and compare the antiulcer and cytotoxic activities of C. procera leaf, flower, and fruit extracts in an attempt to verify its traditional uses. Phytochemical studies on the fruits, flowers, and leaves of C. procera, collected from the desert of Saudi Arabia, led to the isolation of one new lignan 7′-methoxy-3′-O-demethyl-tanegool-9-O-β-D-glucopyranoside and five known compounds from the flowers, four compounds from leaves, and a flavonoid glycoside and a lignan glycoside from the fruits. The structures of compounds were determined by spectroscopic techniques. Ethanol extracts of the three parts of C. procera were evaluated for their antiulcer activity and we found that the leaf extract possessed a powerful antiulcer activity which could be considered as a promising drug candidate. All the extracts and the isolated compounds were evaluated for their cytotoxic activity against MCF-7, HCT-116, HepG-2, and A-549 human cancer cell lines. Compound 2 was highly active on all the cell lines, whereas compounds 5 and 11 were more selective on colon and liver cell lines. Compound 10 demonstrated a significant activity on liver and lung cancer cell lines.

  4. [GPC Fingerprint Chromatograms of Aloe vera Leaf Gel Polysaccharides].

    Science.gov (United States)

    Wang, Qiao-e; Xie, Dan; Qian, Jie; Dong, Yin-mao

    2015-10-01

    To establish the gel permeation chromatography (GPC) fingerprint chromatograms of polysaccharides in Aloe vera leaf gel from the same habitat (Beijing) and different habitats for evaluating the quality of Aloe vera leaf gel products commercially available and testing common adulterated substances. The samples were prepared by water-extraction and alcohol-precipitation method. GPC separation was performed on a Shodex SUGAR KS-805 (300 mm x 8.0 mm, 7 μm) column and a Shodex SUGAR KS-803 (300 mm x 8.0 mm, 6 μm) column at the temperature of 60 degrees C by eluting with 0.1 mol/L NaNO3 (containing 0.2 per thousand NaN) at a flow rate of 0.8 mL/min, the chromatographic effluent was detected by refractive index detector (RID) at the temperature of 50 degrees C. The common pattern of GPC fingerprint chromatograms was established and four common peaks were demarcated. The similarities of samples from the same habitat (Beijing) and different habitats were over 0.9. Taking the GPC fingerprint chromatograms for the qualified model, three commercially available aloe products were evaluated to be made of Aloe vera by the different manufacturing processes and four common adulterated substances of aloe polysaccharides were identified effectively. The method is simple and accurate with a good reproducibility, and it can be used for the identification and quality evaluation of Aloe vera leaf gel products.

  5. UPLC-DAD/Q-TOF-MS Based Ingredients Identification and Vasorelaxant Effect of Ethanol Extract of Jasmine Flower

    Directory of Open Access Journals (Sweden)

    Yongqiang Yin

    2014-01-01

    Full Text Available Chinese people commonly make jasmine tea for recreation and health care. Actually, its medicinal value needs more exploration. In this study, vasorelaxant effect of ethanol extract of jasmine flower (EEJ on isolated rat thoracic aorta rings was investigated and [Ca2+] was determined in vascular smooth muscle cells by laser scanning confocal microscope (LSCM. The result of aorta rings showed that EEJ could cause concentration-dependent relaxation of endothelium-intact rings precontracted with phenylephrine or KCl which was attenuated after preincubation of the rings with L-NAME and three different K+ channel inhibitors; however, indomethacin and glibenclamide did not affect the vasodilatation of EEJ. In addition, EEJ could inhibit contraction induced by PE on endothelium-denuded rings in Ca2+-free medium as well as by accumulation of Ca2+ in Ca2+-free medium with high K+. LSCM also showed that EEJ could lower the elevated level of [Ca2+] induced by KCl. These indicate that the vasodilation of EEJ is in part related to causing the release of nitric oxide, activation of K+ channels, inhibition of influx of excalcium, and release of calcium from sarcoplasmic reticulum. A total of 20 main ingredients, were identified in EEJ by UPLC-DAD/Q-TOF-MS. The vasodilation activity should be attributed to the high content of flavonoid glycosides and iridoid glycosides found in EEJ.

  6. Assessment of Euphorbia hirta L. leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality.

    Science.gov (United States)

    Rajeh, Mohammad Abu Basma; Zuraini, Zakaria; Sasidharan, Sreenivasan; Latha, Lachimanan Yoga; Amutha, Santhanam

    2010-08-31

    The antimicrobial activities of the methanolic extracts of Euphorbia hirta L leaves, flowers, stems and roots were evaluated against some medically important bacteria and yeast using the agar disc diffusion method. Four Gram positive (Staphylococcus aureus, Micrococcus sp., Bacillus subtilis and Bacillus thuringensis), four Gram negative (Escherichia coli, Klebsiella pneumonia, Salmonella typhi and P. mirabilis) and one yeast (Candida albicans) species were screened. Inhibition zones ranged between 16-29 mm. Leaves extract inhibited the growth of all tested microorganisms with large zones of inhibition, followed by that of flowers, which also inhibited all the bacteria except C. albicans. The most susceptible microbes to all extracts were S. aureus and Micrococcus sp. Root extract displayed larger inhibition zones against Gram positive bacteria than Gram negative bacteria and had larger inhibition zones compared to stem extract. The lowest MIC values were obtained with E. coli and C. albicans (3.12 mg/mL), followed by S. aureus (12.50 mg/mL) and P. mirabilis (50.00 mg/mL). All the other bacteria had MIC values of 100.00 mg/mL. Scanning Electron Microscopic (SEM) studies revealed that the cells exposed to leaf extract displayed a rough surface with multiple blends and invaginations which increased with increasing time of treatment, and cells exposed to leaf extract for 36 h showed the most damage, with abundant surface cracks which may be related to final cell collapse and loss of function. Time-kill assay of C. albicans indicated a primarily fungicidal effect at 1- and 2-fold MIC. E. hirta extracts had LC(50) values of 0.71, 0.66, 0.41 and 0.03 mg/mL for stems, leaves, roots and flowers, respectively against Artemia salina. Hence, these plants can be used to discover new bioactive natural products that may serve as leads in the development of new pharmaceuticals.

  7. Design, formulation and evaluation of Aloe vera chewing gum

    Science.gov (United States)

    Aslani, Abolfazl; Ghannadi, Alireza; Raddanipour, Razieh

    2015-01-01

    Background: Aloe vera has antioxidant, antiinflammatory, healing, antiseptic, anticancer and antidiabetic effects. The aim of the present study was to design and evaluate the formulation of Aloe vera chewing gum with an appropriate taste and quality with the indications for healing oral wounds, such as lichen planus, mouth sores caused by cancer chemotherapy and mouth abscesses as well as reducing mouth dryness caused by chemotherapy. Materials and Methods: In Aloe vera powder, the carbohydrate content was determined according to mannose and phenolic compounds in terms of gallic acid. Aloe vera powder, sugar, liquid glucose, glycerin, sweeteners and different flavors were added to the soft gum bases. In Aloe vera chewing gum formulation, 10% of dried Aloe vera extract entered the gum base. Then the chewing gum was cut into pieces of suitable sizes. Weight uniformity, content uniformity, the organoleptic properties evaluation, releasing the active ingredient in the phosphate buffer (pH, 6.8) and taste evaluation were examined by Latin square method. Results: One gram of Aloe vera powder contained 5.16 ± 0.25 mg/g of phenolic compounds and 104.63 ± 4.72 mg/g of carbohydrates. After making 16 Aloe vera chewing gum formulations, the F16 formulation was selected as the best formulation according to its physicochemical and organoleptic properties. In fact F16 formulation has suitable hardness, lack of adhesion to the tooth and appropriate size and taste; and after 30 min, it released more than 90% of its drug content. Conclusion: After assessments made, the F16 formulation with maltitol, aspartame and sugar sweeteners was selected as the best formulation. Among various flavors used, peppermint flavor which had the most acceptance between consumers was selected. PMID:26605214

  8. Fungi associated with base rot disease of aloe vera (Aloe ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-12-17

    Dec 17, 2008 ... Fungi associated with base rot disease of Aloe vera (syn. Aloe barbadensis) were investigated in Niger. Delta Area of Nigeria. Fungi and their percentage frequency were Aspergillus verocosa 28.03%,. Fusarium oxysporium 24.24%, Plectosphaerella cucumerina 16.67%, Mammeria ehinobotryoides 15.91 ...

  9. Fungi associated with base rot disease of aloe vera ( Aloe ...

    African Journals Online (AJOL)

    Fungi associated with base rot disease of Aloe vera (syn. Aloe barbadensis) were investigated in Niger Delta Area of Nigeria. Fungi and their percentage frequency were Aspergillus verocosa 28.03%, Fusarium oxysporium 24.24%, Plectosphaerella cucumerina 16.67%, Mammeria ehinobotryoides 15.91% and Torula ...

  10. Antiallergic effect of the atomized extract of rhizome of Curcuma longa, flowers of Cordia lutea and leaves of Annona muricata

    Science.gov (United States)

    Arroyo-Acevedo, Jorge; Franco-Quino, Cesar; Ruiz-Ramirez, Eliberto; Chávez-Asmat, Roberto; Anampa-Guzmán, Andrea; Raéz-González, Ernesto; Cabanillas-Coral, José

    2016-01-01

    Introduction Allergies are a problem that greatly affects the population, and hence the use of antiallergic medications is fairly widespread. However, these drugs have many adverse effects. The use of medicinal plants could be an option, but they need to be evaluated. Objective This study was designed to evaluate the antiallergic effect of the atomized extract of rhizome of Curcuma longa, flowers of Cordia lutea, and leaves of Annona muricata. Materials and methods Twenty-four New Zealand white albino rabbits were randomized into 2 groups. Group A received the atomized extract diluted in physiological saline (APS) and group B received it diluted in Freund’s adjuvant (FA). Then, the back of each rabbit was divided into 4 quadrants. The A-I quadrant received only physiological saline. The A-I quadrants of each rabbit conformed the PS group. The following 3 quadrants received the APS in 10 μg/mL, 100 μg/mL, and 1,000 μg/mL, respectively. The B-I quadrant received only FA. The B-I quadrants of each rabbit conformed the FA group. The following 3 quadrants received the AFA in 10 μg/mL, 100 μg/mL, and 1,000 μg/mL, respectively. The occurrence of erythema and edema was recorded according to the Draize scoring system and the primary irritation index. After 72 hours, biopsies were performed. Results The AFA group presented significantly less erythema and edema compared to the FA group (Pmuricata lacks antigenic effect but could have an antiallergenic effect in a model of dermal irritation in rabbits. PMID:27877047

  11. Chamomile flower extract-directed CuO nanoparticle formation for its antioxidant and DNA cleavage properties

    International Nuclear Information System (INIS)

    Duman, Fatih; Ocsoy, Ismail; Kup, Fatma Ozturk

    2016-01-01

    In this study, we report the synthesis of copper oxide nanoparticles (CuO NPs) using a medicinal plant (Matricaria chamomilla) flower extract as both reducing and capping agent and investigate their antioxidant activity and interaction with plasmid DNA (pBR322).The CuO NPs were characterized using Uv–Vis spectroscopy, FT-IR (Fourier transform infrared spectroscopy), DLS (dynamic light scattering), XRD (X-ray diffraction), EDX (energy-dispersive X-ray) spectroscopy and SEM (scanning electron microscopy). The CuO NPs exhibited nearly mono-distributed and spherical shapes with diameters of 140 nm size. UV–Vis absorption spectrum of CuO NPs gave a broad peak around 285 and 320 nm. The existence of functional groups on the surface of CuO NPs was characterized with FT-IR analysis. XRD pattern showed that the NPs are in the form of a face-centered cubic crystal. Zeta potential value was measured as − 20 mV due to the presence of negatively charged functional groups in plant extract. Additionally, we demonstrated concentration-dependent antioxidant activity of CuO NPs and their interaction with plasmid DNA. We assumed that the CuO NPs both cleave and break DNA double helix structure. - Highlights: • The synthesis of microwave assisted green synthesis of CuO nanoparticles • The synthesized nanoparticles were analyzed by FT-IR, DLS, XRD, EDX and SEM. • Concentration-dependent antioxidant activity of CuO NPs was determined. • CuO NPs cause both cleavage in the DNA double helix structure and breaks as well.

  12. Antiallergic effect of the atomized extract of rhizome of Curcuma longa, flowers of Cordia lutea and leaves of Annona muricata.

    Science.gov (United States)

    Arroyo-Acevedo, Jorge; Franco-Quino, Cesar; Ruiz-Ramirez, Eliberto; Chávez-Asmat, Roberto; Anampa-Guzmán, Andrea; Raéz-González, Ernesto; Cabanillas-Coral, José

    2016-01-01

    Allergies are a problem that greatly affects the population, and hence the use of antiallergic medications is fairly widespread. However, these drugs have many adverse effects. The use of medicinal plants could be an option, but they need to be evaluated. This study was designed to evaluate the antiallergic effect of the atomized extract of rhizome of Curcuma longa , flowers of Cordia lutea , and leaves of Annona muricata . Twenty-four New Zealand white albino rabbits were randomized into 2 groups. Group A received the atomized extract diluted in physiological saline (APS) and group B received it diluted in Freund's adjuvant (FA). Then, the back of each rabbit was divided into 4 quadrants. The A-I quadrant received only physiological saline. The A-I quadrants of each rabbit conformed the PS group. The following 3 quadrants received the APS in 10 μg/mL, 100 μg/mL, and 1,000 μg/mL, respectively. The B-I quadrant received only FA. The B-I quadrants of each rabbit conformed the FA group. The following 3 quadrants received the AFA in 10 μg/mL, 100 μg/mL, and 1,000 μg/mL, respectively. The occurrence of erythema and edema was recorded according to the Draize scoring system and the primary irritation index. After 72 hours, biopsies were performed. The AFA group presented significantly less erythema and edema compared to the FA group ( P muricata lacks antigenic effect but could have an antiallergenic effect in a model of dermal irritation in rabbits.

  13. Chamomile flower extract-directed CuO nanoparticle formation for its antioxidant and DNA cleavage properties

    Energy Technology Data Exchange (ETDEWEB)

    Duman, Fatih, E-mail: fduman@erciyes.edu.tr [Erciyes University, Science Faculty, Biology Department, Kayseri 38039, Kayseri (Turkey); Ocsoy, Ismail [Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039, Kayseri (Turkey); Erciyes University, Nanotechnology Research Center, 38039, Kayseri (Turkey); Kup, Fatma Ozturk [Erciyes University, Science Faculty, Biology Department, Kayseri 38039, Kayseri (Turkey)

    2016-03-01

    In this study, we report the synthesis of copper oxide nanoparticles (CuO NPs) using a medicinal plant (Matricaria chamomilla) flower extract as both reducing and capping agent and investigate their antioxidant activity and interaction with plasmid DNA (pBR322).The CuO NPs were characterized using Uv–Vis spectroscopy, FT-IR (Fourier transform infrared spectroscopy), DLS (dynamic light scattering), XRD (X-ray diffraction), EDX (energy-dispersive X-ray) spectroscopy and SEM (scanning electron microscopy). The CuO NPs exhibited nearly mono-distributed and spherical shapes with diameters of 140 nm size. UV–Vis absorption spectrum of CuO NPs gave a broad peak around 285 and 320 nm. The existence of functional groups on the surface of CuO NPs was characterized with FT-IR analysis. XRD pattern showed that the NPs are in the form of a face-centered cubic crystal. Zeta potential value was measured as − 20 mV due to the presence of negatively charged functional groups in plant extract. Additionally, we demonstrated concentration-dependent antioxidant activity of CuO NPs and their interaction with plasmid DNA. We assumed that the CuO NPs both cleave and break DNA double helix structure. - Highlights: • The synthesis of microwave assisted green synthesis of CuO nanoparticles • The synthesized nanoparticles were analyzed by FT-IR, DLS, XRD, EDX and SEM. • Concentration-dependent antioxidant activity of CuO NPs was determined. • CuO NPs cause both cleavage in the DNA double helix structure and breaks as well.

  14. Biosynthesis of palladium nanoparticles by using Moringa oleifera flower extract and their catalytic and biological properties.

    Science.gov (United States)

    Anand, K; Tiloke, C; Phulukdaree, A; Ranjan, B; Chuturgoon, A; Singh, S; Gengan, R M

    2016-12-01

    The biosynthesis of nanostructured biopalladium nanoparticles (PdNPs) from an aqueous solution of crystalline palladium acetate is reported. For the synthesised PdNPs in solution, an agroforest biomass waste petal of Moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. Continuous absorption in the UV region and subsequent brown colour change confirmed the formation of PdNPs. A strong surface plasmon peak for PdNPs occurred at 460nm. PdNPs were characterized by SEM with EDX, FTIR, TEM and DLS. The chemical composition of the aqueous extract was determined by GC-MS coupled with FTIR and 1 NMR. The catalytic degradation effect by PdNPs on industrial organic toxic effluents p-nitrophenol (PNP) and methylene blue dye was monitored by UV Spectroscopy. On the other hand PdNPs catalysed the base mediated suzuki coupling reaction for biphenyl synthesis, in water. Moreover, PdNPs were found to be reusable catalysts. Toxicity studies of PdNPs showed that the death of brine shrimp to be <50%. Therefore, PdNPs displayed potential for further anticancer studies via tumour cell lines. The in vitro cytotoxicity evaluation of the extract capped nanoparticles was carried out using human lung carcinoma cells (A549) and peripheral lymphocytes normal cells by MTT cell viability assay. Also, PdNPs showed antibacterial activity against Enterococcus faecalis among the different tested strains, including Bacillus cereus, Staphylococcus aureus, Esherichia coli and Candida albicans, Candida utilis. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. The N-butyl alcohol extract from Hibiscus rosa-sinensis L. flowers enhances healing potential on rat excisional wounds.

    Science.gov (United States)

    Shen, Hui-Min; Chen, Chun; Jiang, Ji-Yang; Zheng, Yi-Lin; Cai, Wen-Feng; Wang, Bin; Ling, Zhen; Tang, Liu; Wang, Yuan-Hang; Shi, Gang-Gang

    2017-02-23

    Hibiscus rosa-sinensis L. (HRS), a folk medicine named Zhujin in China, possess anti-tumor, antioxidant, antibacterial, low density lipoprotein oxidation prevention and macrophage death prevention effects. The leaves and red flowers of HRS have been traditionally used to treat with furuncle and ulceration. To investigate the efficacy and possible mechanism of the N-butyl alcohol extract of HRS (NHRS) red flowers in wound healing by analyzing the collagen fiber deposition, angiogenic activity and macrophages action of the NHRS. In an excisional wound healing model in rats, different concentrations of NHRS, or recombinant bovine basic fibroblast growth factor (rbFGF), were respectively applied twice daily for 9 days. Histopathology was assessed on day 9 via hematoxylin and eosin (HE) and Masson's trichrome (MT) staining, and immunohistochemistry for vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) and CD68. Immunomodulation by NHRS was evaluated by a carbon clearance test in mice. Wound healing post-surgery was greater in the rbFGF-control, NHRS-M and MHRS-H groups than in the model and 5% dimethylsulfoxide (DMSO)-control groups after the third day. By the sixth day the wound contraction of NHRS-M and MHRS-H groups was much higher than the rbFGF-control group. HE and MT staining revealed that epithelialization, fibroblast distribution, collagen deposition of NHRS-M- and NHRS-H-control groups were significantly higher than the model group. Moreover, immunohistochemistry showed more intense staining of VEGF, TGF-β1 and CD68 in the rbFGF- and NHRS-control groups, compared to that in model and 5% DMSO-control groups. The clearance and phagocytic indices of NHRS-M- and NHRS-H-control groups were significantly higher than that of the carboxyl methyl cellulose (CMC) group in mice. NHRS accelerates wound repair via enhancing the macrophages activity, accelerating angiogenesis and collagen fiber deposition response mediated by VEGF and TGF

  16. An Ecofriendly Initiative for the Corrosion Inhibition of Mild Steel in 1 M HCl Using Tecoma capensis Flower Extract

    OpenAIRE

    Prithiba, A.; Rajalakshmi, R.

    2016-01-01

    Corrosion inhibition of mild steel in 1 M HCl in the presence of Tecoma capensis flower extract was carried out by means of mass loss, potentiodynamic polarisation, and electrochemical impedance techniques. The inhibition efficiency varied with concentration of the inhibitor, immersion time, and temperature. The adsorption of the inhibitor on mild steel surface obeys Langmuir’s adsorption isotherm. Thermodynamic parameters reveal that the adsorption process is spontaneous. Electrochemical stu...

  17. Aloes of the world project

    DEFF Research Database (Denmark)

    Klopper, Ronell R.; Smith, Gideon F.; Crouch, Neil R.

    2013-01-01

    Background - The Old World genus Aloe L. comprises ± 630 species to which almost 1300 names have been applied. Members of the genus are prominent components of many, mainly arid, African landscapes. Aloes can be found in Africa (the majority of species), the Arabian Peninsula, Socotra, Madagascar...

  18. Floral Biosynthesis of Mn3O4 and Fe2O3 Nanoparticles Using Chaenomeles sp. Flower Extracts for Efficient Medicinal Applications

    Science.gov (United States)

    Karunakaran, Gopalu; Jagathambal, Matheswaran; Kolesnikov, Evgeny; Dmitry, Arkhipov; Ishteev, Artur; Gusev, Alexander; Kuznetsov, Denis

    2017-08-01

    Manganese oxide (Mn3O4) and iron oxide (Fe2O3) nanoparticles were successfully synthesized with the flower extracts of Chaenomeles sp. This is the first ever approach to synthesize nanoparticles from Chaenomeles sp. flower extracts. The organic molecules present in the flower extracts actively converted the nitrate precursor into its corresponding nanoparticles. The organic molecules that are involved in the synthesis of nanoparticles are identified using different phytochemical and gas chromatography-mass spectrometry analyses. The identified components are glycosides, alkaloids, terpenoids, saponins, flavonoids, quinines, and steroids. The structural and chemical compositions of the synthesized powder were also analyzed. The x-ray powder diffraction analysis revealed that the particles show tetragonal and rhombohedral crystalline phases. The Fourier transform infrared spectroscopy analysis showed the functional groups that are involved in the reduction of nitrates into the corresponding nanoparticles. Energy-dispersive x-ray spectroscopy analysis confirmed the presence of the elements in the synthesized nanoparticles. Transmission electron microscopy images showed the formation of spherical nanoparticles with an average size of 30-100 nm. Antioxidant analysis showed that the synthesized nanoparticles had excellent antioxidant potential. The antibacterial study showed that they inhibit the growth of harmful bacteria such as Pseudomonas aeruginosa and Streptococcus pyogenes. Thus, this study proposes a new eco-friendly and nontoxic method to synthesize nanoparticles for medicinal applications.

  19. Antioxidant and Antimicrobial Attributes and Phenolics of Different Solvent Extracts from Leaves, Flowers and Bark of Gold Mohar [Delonix regia (Bojer ex Hook. Raf.

    Directory of Open Access Journals (Sweden)

    Qaiser M. Khan

    2011-08-01

    Full Text Available This paper describes the antioxidant and antimicrobial activities and phenolic components of different solvent (absolute methanol, absolute ethanol, absolute acetone, 80% methanol, 80% ethanol, 80% acetone and deionized water extracts of leaves, flowers and bark of Gold Mohar [Delonix regia (Bojer ex Hook. Raf.]. The extract yields from leaves, flowers and bark ranged from 10.19 to 36.24, 12.97 to 48.47 and 4.22 to 8.48 g/100 g dry weight (DW, respectively. Overall, 80% methanol extract produced from the leaves exhibited significantly (P < 0.05 higher antioxidant activity, with high phenolic contents (3.63 g GAE/100 g DW, total flavonoid contents (1.19 g CE/100 g DW, inhibition of peroxidation (85.54%, DPPH scavenging capacity (IC50 value 8.89 μg/mL and reducing power (1.87. Similarly, this 80% methanol leaves extract also showed superior antimicrobial activity. HPLC analysis of the 80% methanol extracts for individual phenolics revealed the presence of gallic, protocatechuic and salicylic acid in leaves; gallic, protocatechuic, salicylic, trans-cinnamic and chlorogenic acid in flowers, and gallic acid in bark as the main (amount > 1.50 mg/100 g DW phenolic acids. Besides, small amounts ( < 1.50 mg/100 g DW of some other phenolic acids such as sorbic, sinapic, p-coumaric, m-coumaric, ferulic, caffeic, 3-hydroxybenzoic, 4-hydroxycinnamic and 4-hydroxybenzoic acids were also detected. The extracts of the tested parts of Gold Mohar, especially, the leaves, might be valuable for functional food and therapeutic applications.

  20. Assessment of Euphorbia hirta L. Leaf, Flower, Stem and Root Extracts for Their Antibacterial and Antifungal Activity and Brine Shrimp Lethality

    Directory of Open Access Journals (Sweden)

    Santhanam Amutha

    2010-08-01

    Full Text Available The antimicrobial activities of the methanolic extracts of Euphorbia hirta L leaves, flowers, stems and roots were evaluated against some medically important bacteria and yeast using the agar disc diffusion method. Four Gram positive (Staphylococcus aureus, Micrococcus sp., Bacillus subtilis and Bacillus thuringensis, four Gram negative (Escherichia coli, Klebsiella pneumonia, Salmonella typhi and P. mirabilis and one yeast (Candida albicans species were screened. Inhibition zones ranged between 16–29 mm. Leaves extract inhibited the growth of all tested microorganisms with large zones of inhibition, followed by that of flowers, which also inhibited all the bacteria except C. albicans. The most susceptible microbes to all extracts were S. aureus and Micrococcus sp. Root extract displayed larger inhibition zones against Gram positive bacteria than Gram negative bacteria and had larger inhibition zones compared to stem extract. The lowest MIC values were obtained with E. coli and C. albicans (3.12 mg/mL, followed by S. aureus (12.50 mg/mL and P. mirabilis (50.00 mg/mL. All the other bacteria had MIC values of 100.00 mg/mL. Scanning Electron Microscopic (SEM studies revealed that the cells exposed to leaf extract displayed a rough surface with multiple blends and invaginations which increased with increasing time of treatment, and cells exposed to leaf extract for 36 h showed the most damage, with abundant surface cracks which may be related to final cell collapse and lossThe antimicrobial activities of the methanolic extracts of Euphorbia hirta L leaves, flowers, stems and roots were evaluated against some medically important bacteria and yeast using the agar disc diffusion method. Four Gram positive (Staphylococcus aureus, Micrococcus sp., Bacillus subtilis and Bacillus thuringensis, four Gram negative (Escherichia coli, Klebsiella pneumonia, Salmonella typhi and P. mirabilis and one yeast (Candida albicans species were screened. Inhibition

  1. Multi-responses extraction optimization combined with high-performance liquid chromatography-diode array detection-electrospray ionization-tandem mass spectrometry and chemometrics techniques for the fingerprint analysis of Aloe barbadensis Miller.

    Science.gov (United States)

    Zhong, Jia-Sheng; Wan, Jin-Zhi; Ding, Wen-Jing; Wu, Xiao-Fang; Xie, Zhi-Yong

    2015-03-25

    A quality control strategy using high-performance liquid chromatography-diode array detector-electrospray ionization-tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) coupled with chemometrics analysis was proposed for Aloe barbadensis Miller. Firstly, the extraction conditions including methanol concentration, extraction time and solvent-to-material ratio were optimized by multi-responses optimization based on response surface methodology (RSM). The optimum conditions were achieved by Derringer's desirability function and experimental validation implied that the established model exhibited favorable prediction ability. Then, HPLC fingerprint consisting of 27 common peaks was developed among 15 batches of A. barbadensis samples. 25 common peaks were identified using HPLC-DAD-ESI-MS/MS method by their spectral characteristics or comparison with the authentic standards. Chemometrics techniques including similarity analysis (SA), principal components analysis (PCA) and hierarchical clustering analysis (HCA) were implemented to classify A. barbadensis samples. The results demonstrated that all A. barbadensis samples shared similar chromatographic patterns as well as differences. These achievements provided an effective, reliable and comprehensive quality control method for A. barbadensis. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Methanolic extract of Woodfordia fruticosa Kurz flowers ameliorates carbon tetrachloride-induced chronic hepatic fibrosis in rats.

    Science.gov (United States)

    Nitha, A; Prabha, S P; Ansil, P N; Latha, M S

    2016-07-01

    Hepatic fibrosis, characterized by extracellular matrix accumulation, is the common cause of chronic liver failure and is a leading cause of morbidity and mortality worldwide. The aim of the present study was to evaluate the effect of dried flowers of Woodfordia fruticosa on carbon tetrachloride (CCl4)-induced hepatic fibrosis in rat model. Hepatic fibrosis was induced in male Wistar rats by CCl4 administration (150 μl/100 g rat weight, oral) twice a week for 10 weeks. In preventive model, administration of daily doses of methanolic extract of W. fruticosa (MEWF) at two different doses (100 mg/kg, body weight (b.w.) and 200 mg/kg, b.w.) was started 1 week before the onset of CCl4 administration and continued for 10 weeks. In curative model, MEWF at 100 and 200 mg/kg were given for last 2 weeks after the establishment of fibrosis. MEWF at a dose of 200 mg/kg was able to exert a more pronounced effect as evidenced histologically by significant reduction in fibrotic septa formation in liver tissue, immunohistochemically by abridged expression of collagen III, and also biochemically by serum and tissue antioxidant status, lipid peroxidation, and hydroxyproline level. Liquid chromatography-mass spectrometry analysis revealed the presence of confertin, quercetin methyl ether, ellagic acid, and stigmasterol in MEWF, which could be responsible for its antifibrotic activity. These results indicate the effective protection exerted by MEWF against CCl4-induced hepatic fibrosis in a dose-dependent manner. © The Author(s) 2014.

  3. Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.

    Science.gov (United States)

    Nedel, Fernanda; Begnini, Karine; Carvalho, Pedro Henrique de Azambuja; Lund, Rafael Guerra; Beira, Fátima T A; Del Pino, Francisco Augusto B

    2012-11-01

    This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3.

  4. Potential therapeutic activity of Phlogacanthus thyrsiformis Hardow (Mabb) flower extract and its biofabricated silver nanoparticles against chemically induced urolithiasis in male Wistar rats.

    Science.gov (United States)

    Das, Poppy; Kumar, Kiran; Nambiraj, Arunai; Rajan, Reshma; Awasthi, Rajendra; Dua, Kamal; M, Himaja

    2017-10-01

    Urolithiasis is a painful disorder in which stones are formed in the kidney, bladder or urethra. There are no proper therapeutic treatments available for kidney stones and people suffering from larger stones have to undergo surgery which has many side effects. A natural remedy with therapeutic effects that can dissipate and remove even the larger stones would eliminate the need of a surgery and the risks associated with it. The flowers of Phlogacanthus thyrsiformis used in culinary recipes in the north eastern India are also widely used as a folklore medicine for the treatment of kidney stones and liver disorders. The aim of this study was to evaluate the prophylactic and therapeutic activity of the aqueous extract of P. thyrsiformis flowers and its biofabricated silver nanoparticles against struvite urinary stones and calcium oxalate kidney stones. A kidney stone inhibition study was carried out on struvite stones grown in gel medium and calcium oxalate stones in rat models using an aqueous extract of P. thyrsiformis flowers and its biofabricated silver nanoparticles. The aqueous extract of P. thyrsiformis flowers and their biofabricated silver nanoparticles, obtained by a green synthetic method, were used to treat struvite urinary stones in vitro and calcium oxalate kidney stones in vivo. Struvite stones were grown in tubes by gel diffusion technique and were treated with varying concentrations of the extract and its nanoparticles. The size of the struvite stones was monitored for 96h using a travelling microscope. Calcium oxalate stones were induced in male Wistar rats by feeding ethylene glycol-ammonium chloride mixture for 14days. Both, prophylactic and therapeutic activities were evaluated by analyzing the urine, serum and histopathological parameters of the rats. The qualitative screening of water extract unveiled the presence of flavonoids as a major constituent. Both, the extract and the nanoparticles effectively reduced the size of struvite stones in

  5. Nanocrystalline spinel ferrite (MFe{sub 2}O{sub 4}, M = Ni, Co, Mn, Mg, Zn) powders prepared by a simple aloe vera plant-extracted solution hydrothermal route

    Energy Technology Data Exchange (ETDEWEB)

    Phumying, Santi; Labuayai, Sarawuth; Swatsitang, Ekaphan; Amornkitbamrung, Vittaya [Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Integrated Nanotechnology Research Center (INRC), Khon Kaen University, Khon Kaen 40002 (Thailand); Maensiri, Santi, E-mail: santimaensiri@gmail.com [School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000 (Thailand)

    2013-06-01

    Graphical abstract: This figure shows the specific magnetization curves of the as-prepared MFe{sub 2}O{sub 4} (M = Ni, Co, Mn, Mg, Zn) powders obtained from room temperature VSM measurement. These curves are typical for a soft magnetic material and indicate hysteresis ferromagnetism in the field ranges of ±500 Oe, ±1000 Oe, and ±2000 Oe for the CoFe{sub 2}O{sub 4}, MgFe{sub 2}O{sub 4} and MnFe{sub 2}O{sub 4} respectively, whereas the samples of NiFe{sub 2}O{sub 4} and ZnFe{sub 2}O{sub 4} show a superparamagnetic behavior. Highlights: ► Nanocrystalline MFe{sub 2}O{sub 4} powders were synthesized by a novel hydrothermal method. ► Metal acetylacetonates and aloe vera plant-extracted solution are used. ► This biosynthetic route is very simple and provides high-yield oxide nanomaterials. ► XRD and TEM results indicate that the prepared samples have only spinel structure. ► The maximum M{sub s} of 68.9 emu/g at 10 kOe were observed for the samples of MnFe{sub 2}O{sub 4}. - Abstract: Nanocrystalline spinel ferrite MFe{sub 2}O{sub 4} (M = Ni, Co, Mn, Mg, Zn) powders were synthesized by a novel hydrothermal method using Fe(acac){sub 3}, M(acac){sub 3} (M = Ni, Co, Mn, Mg, Zn) and aloe vera plant extracted solution. The X-ray diffraction and selected-area electron diffraction results indicate that the synthesized nanocrystalline have only spinel structure without the presence of other phase impurities. The crystal structure and morphology of the spinel ferrite powders, as revealed by TEM, show that the NiFe{sub 2}O{sub 4} and CoFe{sub 2}O{sub 4} samples contain nanoparticles, whereas the MnFe{sub 2}O{sub 4} and MgFe{sub 2}O{sub 4} samples consist of many nanoplatelets and nanoparticles. Interestingly, the ZnFe{sub 2}O{sub 4} sample contains plate-like structure of networked nanocrystalline particles. Room temperature magnetization results show a ferromagnetic behavior of the CoFe{sub 2}O{sub 4}, MnFe{sub 2}O{sub 4} and MgFe{sub 2}O{sub 4} samples, whereas the

  6. Antioxidant compounds and activities of the stem, flower, and leaf extracts of the anti-smoking Thai medicinal plant: Vernonia cinerea Less

    Directory of Open Access Journals (Sweden)

    Ketsuwan N

    2017-02-01

    Full Text Available Nitinet Ketsuwan,1 Jirakrit Leelarungrayub,1 Suchart Kothan,2 Supawatchara Singhatong3 1Department of Physical Therapy, 2Department of Radiologic Technology, 3Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand Abstract: Vernonia cinerea (VC Less has been proposed as a medicinal plant with interesting activities, such as an aid for smoking cessation worldwide. Despite its previous clinical success in smoking cessation by exhibiting reduced oxidative stress, it has not been approved. The aim of this study was to investigate various antioxidant activity and active compounds that have not been approved, including the protective activity in human red blood cells (RBCs, from the stem, flower, and leaf extracts of VC Less in vitro. These extracts were tested for their antioxidant activity in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH radicals and analyzed by high-performance liquid chromatography (HPLC for their active compounds: total tannin, five catechin (C compounds (epicatechin gallate [ECG], C, epicatechin [EC], epigallocatechin gallate [EGCG], and (--epigallocatechin [EGC], flavonoid, nitrite, nitrate, caffeine, and nicotine. Moreover, antioxidant activities of the extracts were evaluated in 2,2'-azobis(2-amidinopropane dihydrochloride (AAPH-treated RBCs. The results showed that the flower and leaf of VC Less had higher activity than the stem in scavenging DPPH radicals. The tannin content in the flower and leaf was higher than that in the stem. The leaf had the highest content of the five catechins (C, EC, EGCG, ECG, and EGC, the same as in the flavonoid, when compared to the stem and flower. Furthermore, the leaf extract had higher nitrate and nitrite than the stem. Nicotine content was found to be higher in the leaf when compared to the flower. In addition, the leaf showed protective activity in glutathione (GSH, malondialdehyde (MDA, and protein carbonyl, with a dose

  7. Flowering Trees

    Indian Academy of Sciences (India)

    Baccaurea courtallensis Muell.-Arg. of Euphorbiaceae is an evergreen tree that is very attractive when in flower. Leaves are alternate. Male and female flowers are borne on separate trees. Inflorescences bearing several flowers arise in tufts on tubercles on the stem. Fruits are crimson red in colour. Seeds are covered.

  8. Blob Flowers.

    Science.gov (United States)

    Canfield, Elaine

    2003-01-01

    Describes an art project called blob flowers in which fifth-grade students created pictures of flowers using watercolor and markers. Explains that the lesson incorporates ideas from art and science. Discusses in detail how the students created their flowers. (CMK)

  9. Dye-Sensitized Solar Cells Employing Extracts from Four Cassia Flowers as Natural Sensitizers: Studies on Dye Ingredient Effect on Photovoltaic Performance

    Science.gov (United States)

    Maurya, Ishwar Chandra; Singh, Shalini; Neetu; Gupta, Arun Kumar; Srivastava, Pankaj; Bahadur, Lal

    2018-01-01

    Natural dyes extracted from four different flowers, namely, Cassia surattensis, Cassia tora, Cassia alata and Cassia occidentalis were used as sensitizers for TiO2-based dye-sensitized solar cells (DSSC). The dye extracts from flowers were obtained by a simple extraction technique and used without any further purification. Optical characteristics of dye extracts were studied. Fourier-transform infrared (FTIR) spectra were used to identify the constituents of extracted dyes. The photovoltaic performance of DSSC employing dye-capped TiO2 photoanodes was measured. The sensitization performance related to anchoring groups present and interaction between dyes with TiO2 surface is demonstrated. An attempt has been made to rationalize the observations by light absorption of the dye extracts and their adsorption on TiO2. The short-circuit current density ( I SC) values ranged from 0.06 mA/cm2 to 0.20 mA/cm2; open circuit voltage ( V OC) from 0.292 V to 0.833 V; fill factor (FF) from 0.7 to 0.9; efficiencies ( η) from 0.013% to 0.15% and incident photon-to-current conversion efficiency from 13% to 20%, were obtained for DSSC using these natural dye extracts. Cassia occidentalis showed the highest current density of 0.20 mA/cm2 and power conversion efficiency of 0.15%, which was due to better interaction between the carbonyl and hydroxyl group of the anthocyanin molecule of C. occidentalis and surface of TiO2 film. The red and blue shift of absorption wavelength of C. surattensis and the blue shift of absorption wavelength of the C. tora, C. alata and C. occidentalis extract in ethanol solution compared to that on TiO2 film has been used for the interpretation of obtained results.

  10. Development and application of UHPLC-MS/MS method for the determination of phenolic compounds in Chamomile flowers and Chamomile tea extracts.

    Science.gov (United States)

    Nováková, Lucie; Vildová, Anna; Mateus, Joana Patricia; Gonçalves, Tiago; Solich, Petr

    2010-09-15

    UHPLC-MS/MS method using BEH C18 analytical column was developed for the separation and quantitation of 12 phenolic compounds of Chamomile (Matricaria recutita L.). The separation was accomplished using gradient elution with mobile phase consisting of methanol and formic acid 0.1%. ESI in both positive and negative ion mode was optimized with the aim to reach high sensitivity and selectivity for quantitation using SRM experiment. ESI in negative ion mode was found to be more convenient for quantitative analysis of all phenolics except of chlorogenic acid and kaempherol, which demonstrated better results of linearity, accuracy and precision in ESI positive ion mode. The results of method validation confirmed, that developed UHPLC-MS/MS method was convenient and reliable for the determination of phenolic compounds in Chamomile extracts with linearity >0.9982, accuracy within 76.7-126.7% and precision within 2.2-12.7% at three spiked concentration levels. Method sensitivity expressed as LOQ was typically 5-20 nmol/l. Extracts of Chamomile flowers and Chamomile tea were subjected to UHPLC-MS/MS analysis. The most abundant phenolic compounds in both Chamomile flowers and Chamomile tea extracts were chlorogenic acid, umbelliferone, apigenin and apigenin-7-glucoside. In Chamomile tea extracts there was greater abundance of flavonoid glycosides such as rutin or quercitrin, while the aglycone apigenin and its glycoside were present in lower amount. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  11. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)

    Extraction of the yellow colour compounds of leaves of Aloe excelsa were performed and 1,8-dihydroxy-3-methylanthracenedione (chrysophanol) was isolated and tested for antibacterial activities against four gram negative and five gram positive bacterial strains. The structures of chrysophanol was determined by chemical ...

  12. Comparative antimicrobial activities of aloe vera gel and leaf ...

    African Journals Online (AJOL)

    The comparative antimicrobial activities of the gel and leaf of Aloe vera were tested against Staphylococcus aureus, Pseudomonas aeruginosa, Trichophyton mentagraphytes, T. schoeleinii, Microsporium canis and Candida albicans. Ethanol was used for the extraction of the leaf after obtaining the gel from it. Antimicrobial ...

  13. Assessment of Chronic Administration of Aloe Vera Gel On ...

    African Journals Online (AJOL)

    The study was designed to investigate the effects of chronic administration of Aloe vera gel extract on markers of hepatic damage, lipid profiles and erythrocyte osmotic fragility using the Wistar rats. Forty male Wistar rats divided into four groups of ten rats per group were used in the study. Group I which served as the control ...

  14. Analyses of Aloe polysaccharides using carbohydrate microarray profiling

    DEFF Research Database (Denmark)

    Isager Ahl, Louise; Grace, Olwen M; Pedersen, Henriette Lodberg

    2018-01-01

    As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another and the effects they have on the human body are increasing. Polysaccharides present in the leaf mesophyll have been...... identified as the components responsible for the biological activities of Aloe vera, and they have been widely studied in the past decades. However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions as most of them require...... a complete or near-complete fractionation of the polymers. The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in Aloe leaf mesophyll. The method we chose is known as Comprehensive Microarray Polymer Profiling...

  15. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study).

    Science.gov (United States)

    Ehsani, Maryam; Amin Marashi, Mahmood; Zabihi, Ebrahim; Issazadeh, Maryam; Khafri, Soraya

    2013-01-01

    Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk diffusion, microdilution and direct contact test. In addition to the above bacterium, the Aloe vera gel effect on Staphylococcus aureus and Streptococcus mutans was evaluated. Disk diffusion test revealed that propolis ethanolic extracts (the alcohol concentration of 15 and 40%) and Aloe vera gel have antibacterial activities but aqueous extract of propolis did not show any effect in this test. The MICs for propolis ethanolic extracts, Aloe vera gel and aqueous extract of propolis (0% alcohol) were 313 µg/ml, 750 µg/ml, 2250 µg/ml, and ≥ 500 µg/ml respectively, much higher than the Chlorhexidine one. In direct contact test, contrary to Aloe vera, all three propolis extracts showed antibacterial effects on E. faecalis. The Aloe vera gel also showed significant antibacterial effect on S.aureus and S.mutans. The hydroalcoholic extracts of propolis and Aloe vera gel had antibacterial effects on E. faecalis, however, propolis is more potent than Aloe vera. The antibacterial effect of Aloe vera on S. aureus and S. mutans is low (MIC ≥ 2250 µg/ml). Appropriate concentrations of alcoholic extracts of propolis and some fractions of Aloe vera gel might be good choices for disinfecting the root canal in endodontic treatments.

  16. Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes.

    Science.gov (United States)

    Moriyama, Mariko; Moriyama, Hiroyuki; Uda, Junki; Kubo, Hirokazu; Nakajima, Yuka; Goto, Arisa; Akaki, Junji; Yoshida, Ikuyo; Matsuoka, Nobuya; Hayakawa, Takao

    2016-01-01

    Aloe has been used as a folk medicine because it has several important therapeutic properties. These include wound and burn healing, and Aloe is now used in a variety of commercially available topical medications for wound healing and skin care. However, its effects on epidermal keratinocytes remain largely unclear. Our data indicated that both Aloe vera gel (AVG) and Cape aloe extract (CAE) significantly improved wound healing in human primary epidermal keratinocytes (HPEKs) and a human skin equivalent model. In addition, flow cytometry analysis revealed that cell surface expressions of β1-, α6-, β4-integrin, and E-cadherin increased in HPEKs treated with AVG and CAE. These increases may contribute to cell migration and wound healing. Treatment with Aloe also resulted in significant changes in cell-cycle progression and in increases in cell number. Aloe increased gene expression of differentiation markers in HPEKs, suggesting roles for AVG and CAE in the improvement of keratinocyte function. Furthermore, human skin epidermal equivalents developed from HPEKs with medium containing Aloe were thicker than control equivalents, indicating the effectiveness of Aloe on enhancing epidermal development. Based on these results, both AVG and CAE have benefits in wound healing and in treatment of rough skin.

  17. Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

    Directory of Open Access Journals (Sweden)

    Samira Negahdari

    2017-01-01

    Conclusions: The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgf β 1 and Vegf-A genes in fibroblast cells treated with extracts.

  18. Subcritical water extraction and antioxidant activity evaluation with on-line HPLC-ABTS(·+) assay of phenolic compounds from marigold (Tagetes erecta L.) flower residues.

    Science.gov (United States)

    Xu, Honggao; Wang, Weiyou; Jiang, Junping; Yuan, Fang; Gao, Yanxiang

    2015-06-01

    Subcritical water extraction (SWE) of phenolics was investigated from marigold (Tagetes erecta L.) flower residues. The total phenolics content (TPC), total flavonoids content (TFC) and antioxidant capacities of extracts were determined, furthermore, antioxidant activities of individual compounds were evaluated with on-line HPLC-ABTS(•+) system. The optimum SWE time was 45 min, solid-to-liquid ratio was 1:50, and the highest TPC and TFC were obtained at 220 °C respectively. The effect of SWE temperature on TPC and TFC was significant (p line HPLC-ABTS(•+) profiles revealed that quercetagetin from SWE at 200 °C had nearly twofold radical scavenging activities than that by leaching extraction.

  19. Ultrasonic-assisted green synthesis of flower like silver nanocolloids using marine sponge extract and its effect on oral biofilm bacteria and oral cancer cell lines.

    Science.gov (United States)

    Inbakandan, D; Kumar, C; Bavanilatha, M; Ravindra, Dune Naga; Kirubagaran, R; Khan, S Ajmal

    2016-10-01

    The knowhow followed for synthesis, characterization and application of nanomaterials has become an important branch of nanoscience. The use of marine sponges for the synthesis of metal nanoparticles is still in the budding level of current nanobiotechnology. This paper reports a single step one pot biosynthesis utilizing marine sponge (Haliclona exigua) extract as a reducing agent by means of a conventional ultrasonic bath on the formation and growth of flower like silver nanocolloids. These silver nanocolloids were characterized through UV visible spectroscopy, High Resolution Transmission Electron Microscope, Fourier Transform Infrared spectroscopy and X-ray Diffractometer. Further, antibacterial activity and antiproliferative activity were done against oral biofilm bacteria and oral cancer cell lines for the biosynthesized flower like silver nanocolloids. Water soluble organic amines were responsible for the syntheses of nanomaterials which have a size range from 100 to 120 nm. An average size of 9.1 mm zone of inhibition was recorded with 10.0 μg of silver nanocolloids against oral biofilm bacteria. The estimated half maximal inhibitory concentration value for flower like silver nanocolloids was 0.6 μg/ml for oral cancer cell lines. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Anti‐Endometriotic Effects of Pueraria Flower Extract  in Human Endometriotic Cells and Mice

    Directory of Open Access Journals (Sweden)

    Ji‐Hyun Kim

    2017-02-01

    Full Text Available Pueraria flowers have been used as a vegetable and an ingredient for tea and jelly. In this study, we investigated the effects of Pueraria flower extract (PFE on endometriosis, a common gynaecological disease characterised by local sterile inflammation of peritoneal cavity. PFE suppressed the adhesion of human endometriotic cells 11Z and 12Z to human mesothelial Met5A cells. In addition, PFE significantly inhibited the migration of 11Z and 12Z cells as shown by woundhealing and transwell migration assays. PFE reduced the protein and mRNA levels of matrix metalloproteinase (MMP‐2 and MMP‐9 in endometriotic cells. Moreover, extracellular signalregulated kinase (ERK1/2 was activated by PFE treatment, and an ERK1/2 inhibitor, PD98059, significantly inhibited PFE‐inhibited cell migration in endometriotic cells. Furthermore, PFE significantly suppressed endometriotic lesion formation in a mouse model. These data suggest that Pueraria flower is a potential anti‐endometriotic agent for the inhibition of endometriotic cell adhesion, migration, and MMP expression.

  1. Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway

    Science.gov (United States)

    Tan, Woan Sean; Arulselvan, Palanisamy; Karthivashan, Govindarajan; Fakurazi, Sharida

    2015-01-01

    Aim of Study. Moringa oleifera Lam. (M. oleifera) possess highest concentration of antioxidant bioactive compounds and is anticipated to be used as an alternative medicine for inflammation. In the present study, we investigated the anti-inflammatory activity of 80% hydroethanolic extract of M. oleifera flower on proinflammatory mediators and cytokines produced in lipopolysaccharide- (LPS-) induced RAW 264.7 macrophages. Materials and Methods. Cell cytotoxicity was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) production was quantified through Griess reaction while proinflammatory cytokines and other key inflammatory markers were assessed through enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Results. Hydroethanolic extract of M. oleifera flower significantly suppressed the secretion and expression of NO, prostaglandin E2 (PGE2), interleukin- (IL-) 6, IL-1β, tumor necrosis factor-alpha (TNF-α), nuclear factor-kappa B (NF-κB), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2). However, it significantly increased the production of IL-10 and IκB-α (inhibitor of κB) in a concentration dependent manner (100 μg/mL and 200 μg/mL). Conclusion. These results suggest that 80% hydroethanolic extract of M. oleifera flower has anti-inflammatory action related to its inhibition of NO, PGE2, proinflammatory cytokines, and inflammatory mediator's production in LPS-stimulated macrophages through preventing degradation of IκB-α in NF-κB signaling pathway. PMID:26609199

  2. Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway

    Directory of Open Access Journals (Sweden)

    Woan Sean Tan

    2015-01-01

    Full Text Available Aim of Study. Moringa oleifera Lam. (M. oleifera possess highest concentration of antioxidant bioactive compounds and is anticipated to be used as an alternative medicine for inflammation. In the present study, we investigated the anti-inflammatory activity of 80% hydroethanolic extract of M. oleifera flower on proinflammatory mediators and cytokines produced in lipopolysaccharide- (LPS- induced RAW 264.7 macrophages. Materials and Methods. Cell cytotoxicity was conducted by 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay. Nitric oxide (NO production was quantified through Griess reaction while proinflammatory cytokines and other key inflammatory markers were assessed through enzyme-linked immunosorbent assay (ELISA and immunoblotting. Results. Hydroethanolic extract of M. oleifera flower significantly suppressed the secretion and expression of NO, prostaglandin E2 (PGE2, interleukin- (IL- 6, IL-1β, tumor necrosis factor-alpha (TNF-α, nuclear factor-kappa B (NF-κB, inducible NO synthase (iNOS, and cyclooxygenase-2 (COX-2. However, it significantly increased the production of IL-10 and IκB-α (inhibitor of κB in a concentration dependent manner (100 μg/mL and 200 μg/mL. Conclusion. These results suggest that 80% hydroethanolic extract of M. oleifera flower has anti-inflammatory action related to its inhibition of NO, PGE2, proinflammatory cytokines, and inflammatory mediator’s production in LPS-stimulated macrophages through preventing degradation of IκB-α in NF-κB signaling pathway.

  3. Flower extract of Allium atroviolaceum triggered apoptosis, activated caspase-3 and down-regulated antiapoptotic Bcl-2 gene in HeLa cancer cell line.

    Science.gov (United States)

    Khazaei, Somayeh; Ramachandran, Vasudevan; Abdul Hamid, Roslida; Mohd Esa, Norhaizan; Etemad, Ali; Moradipoor, Sara; Ismail, Patimah

    2017-05-01

    Cervical cancer accounts for the second most frequent cancer and also third leading cause of cancer mortality (15%) among women worldwide. The major problems of chemotherapeutic treatment in cervical cancer are non-specific cytotoxicity and drug resistance. Plant-derived products, known as natural therapies, have been used for thousands of years in cancer treatment with a very low number of side effects. Allium atroviolaceum is a species in the genus Allium and Liliaceae family, which could prove to have beneficial effects on cancer treatment, although there is a lack of corresponding attention. The methanolic extract from the A.atroviolaceum flower displayed marked anticancer activity on HeLa human cervix carcinoma cells with much lower cytotoxic effects on normal cells (3T3). The A.atroviolaceum extract induced apoptosis, confirmed by cell cycle arrest at the sub-G0 (apoptosis) phase, characteristic morphological changes, evident DNA fragmentation, observed by fluorescent microscope, and early and late apoptosis detection by Annexin V. Furthermore, down-regulation of Bcl-2 and activation of caspase-9 and -3 strongly indicated that the mitochondrial pathway was involved in the apoptosis signal pathway. Moreover, combination of A.atroviolaceum extract with doxorubicin revealed a significant reduction of IC 50 and led to a synergistic effect. In summary, A.atroviolaceum displayed a significant anti-tumour effect through apoptosis induction in HeLa cells, suggesting that the A.atroviolaceum flower might have therapeutic potential against cervix carcinoma. Copyright © 2017. Published by Elsevier Masson SAS.

  4. Bioprospecting for anti-Streptococcus mutans: The activity of 10% Sesbania grandiflora flower extract comparable to erythromycin

    Directory of Open Access Journals (Sweden)

    Azis Saifudin

    2016-09-01

    Conclusions: S. grandiflora flower is a promising material to be developed as the active ingredient of anti-plaque toothpaste as well as mouthwash solution. The developed HPLC and TLC system can be used for a further standard in its material authentication as well as for a fingerprinting of quality control during the manufacturing process.

  5. An Ecofriendly Initiative for the Corrosion Inhibition of Mild Steel in 1 M HCl Using Tecoma capensis Flower Extract

    Directory of Open Access Journals (Sweden)

    A. Prithiba

    2016-01-01

    Full Text Available Corrosion inhibition of mild steel in 1 M HCl in the presence of Tecoma capensis flower extract was carried out by means of mass loss, potentiodynamic polarisation, and electrochemical impedance techniques. The inhibition efficiency varied with concentration of the inhibitor, immersion time, and temperature. The adsorption of the inhibitor on mild steel surface obeys Langmuir’s adsorption isotherm. Thermodynamic parameters reveal that the adsorption process is spontaneous. Electrochemical studies reflect that the inhibitor acts as a mixed-type inhibitor. Surface analytical techniques ascertain the inhibitive nature of the studied inhibitor.

  6. Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis.

    Science.gov (United States)

    Heller, W; Forkmann, G; Britsch, L; Grisebach, H

    1985-08-01

    A cell-free extract from flowers of Matthiola incana catalyzes a NADPH-dependent stereospecific reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4'-trihydroxyflavan-3,4-cis-diol). The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that found with NADPH. (+)-Dihydroquercetin and (+)-dihydromyricetin were also reduced by the enzyme preparation to the corresponding flavan-3,4-cis-diols. Correlation between the genotype of M. incana and the presence of dihydroflavonol 4-reductase is strong evidence that this enzyme is involved in anthocyanin biosynthesis.

  7. Contact and fumigant toxicity of hexane flower bud extract of Syzygium aromaticum and its compounds against Pediculus humanus capitis (Phthiraptera: Pediculidae).

    Science.gov (United States)

    Bagavan, Asokan; Rahuman, Abdul Abdul; Kamaraj, Chinnaperumal; Elango, Gandhi; Zahir, Abdul Abduz; Jayaseelan, Chidambaram; Santhoshkumar, Thirunavukkarasu; Marimuthu, Sampath

    2011-11-01

    The head lice, Pediculus humanus capitis De Geer is an obligate ectoparasite of humans that causes pediculosis capitis, a nuisance for millions of people worldwide, with high prevalence in children. P. humanus capitis has been treated by methods that include the physical remotion of lice, various domestic treatments, and conventional insecticides. None of these methods render complete protection, and there is clear evidence for the evolution of resistance and cross-resistance to conventional insecticides. Non-toxic alternative options are hence needed for head lice treatment and/or prevention, and natural products from plants are good candidates for safer control agents that may provide good anti-lice activity. The plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability. The present study carried out the pediculocidal activity using the hexane flower bud extract of Syzygium aromaticum (Myrtaceae) against P. humanus capitis examined by direct contact and fumigant toxicity (closed- and open-container methods) bioassay. The chemical composition of S. aromaticum flower bud hexane extract was analyzed by gas chromatography-mass spectrometry. The major chemical constituent (58.79%) of flower bud hexane extract S. aromaticum was identified as chavibetol (5-allyl-2-methoxyphenol) by comparison of mass spectral data and retention times. The hexane extract of S. aromaticum was subjected to gas chromatography analysis, and totally 47 compounds were detected, of which chavibetol was predominantly present. The other major constituents present in the hexane extract were eugenol acetate (phenol,2-methoxy-4-(2-propenyl)-,acetate (15.09%), caryophyllene-(I1) (2,6,10,10-tetramethyl bicyclo [7.2.0] undeca-1,6-diene (13.75%), caryophyllene oxide (3.04%), 2,6,6,9-tetramethyl-1,4,8-cycloundecatriene (1.67%), and copaene (1.33%). The filter paper contact bioassay study showed pronounced pediculicidal activity in the flower bud hexane

  8. Aloe vera for treating acute and chronic wounds.

    Science.gov (United States)

    Dat, Anthony D; Poon, Flora; Pham, Kim B T; Doust, Jenny

    2012-02-15

    Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process. To determine the effects of Aloe vera-derived products (for example dressings and topical gels) on the healing of acute wounds (for example lacerations, surgical incisions and burns) and chronic wounds (for example infected wounds, arterial and venous ulcers). We searched the Cochrane Wounds Group Specialised Register (9 September 2011), the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2011, Issue 3), Ovid MEDLINE (2005 to August Week 5 2011), Ovid MEDLINE (In-Process & Other Non-Indexed Citations 8 September 2011), Ovid EMBASE (2007 to 2010 Week 35), Ovid AMED (1985 to September 2011) and EBSCO CINAHL (1982 to 9 September 2011). We did not apply date or language restrictions. We included all randomised controlled trials that evaluated the effectiveness of Aloe vera, aloe-derived products and a combination of Aloe vera and other dressings as a treatment for acute or chronic wounds. There was no restriction in terms of source, date of publication or language. An objective measure of wound healing (either proportion of completely healed wounds or time to complete healing) was the primary endpoint. Two review authors independently carried out trial selection, data extraction and risk of bias assessment, checked by a third review author. Seven trials were eligible for inclusion, comprising a total of 347 participants. Five trials in people with acute wounds evaluated the effects of Aloe vera on burns, haemorrhoidectomy patients and skin biopsies. Aloe vera mucilage did not increase burn healing compared with silver sulfadiazine (risk ratio (RR) 1.41, 95% confidence interval (CI) 0.70 to 2.85). A reduction in healing time with Aloe vera was noted after haemorrhoidectomy (RR 16.33 days, 95% CI 3.46 to 77.15) and there was

  9. Aloe vera for treating acute and chronic wounds

    Directory of Open Access Journals (Sweden)

    Anthony D. Dat

    Full Text Available BACKGROUND: Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process.OBJECTIVE: To determine the effects of Aloe vera-derived products (for example dressings and topical gels on the healing of acute wounds (for example lacerations, surgical incisions and burns and chronic wounds (for example infected wounds, arterial and venous ulcers.METHODS:Search methods: We searched the Cochrane Wounds Group Specialised Register (9 September 2011, the Cochrane Central Register of Controlled Trials (CENTRAL (The Cochrane Library 2011, Issue 3, Ovid MEDLINE (2005 to August Week 5 2011, Ovid MEDLINE (In-Process & Other Non-Indexed Citations 8 September 2011, Ovid EMBASE (2007 to 2010 Week 35, Ovid AMED (1985 to September 2011 and EBSCO CINAHL (1982 to 9 September 2011. We did not apply date or language restrictions. Selection criteria: We included all randomised controlled trials that evaluated the effectiveness of Aloe vera, aloe-derived products and a combination of Aloe vera and other dressings as a treatment for acute or chronic wounds. There was no restriction in terms of source, date of publication or language. An objective measure of wound healing (either proportion of completely healed wounds or time to complete healing was the primary endpoint. Data collection and analysis: Two review authors independently carried out trial selection, data extraction and risk of bias assessment, checked by a third review author.MAIN RESULTS:Seven trials were eligible for inclusion, comprising a total of 347 participants. Five trials in people with acute wounds evaluated the effects of Aloe vera on burns, haemorrhoidectomy patients and skin biopsies. Aloe vera mucilage did not increase burn healing compared with silver sulfadiazine (risk ratio (RR 1.41, 95% confidence interval (CI 0.70 to 2.85. A reduction in

  10. Antidiabetic effects of chamomile flowers extract in obese mice through transcriptional stimulation of nutrient sensors of the peroxisome proliferator-activated receptor (PPAR family.

    Directory of Open Access Journals (Sweden)

    Christopher Weidner

    Full Text Available Given the significant increases in the incidence of metabolic diseases, efficient strategies for preventing and treating of these common disorders are urgently needed. This includes the development of phytopharmaceutical products or functional foods to prevent or cure metabolic diseases. Plant extracts from edible biomaterial provide a potential resource of structurally diverse molecules that can synergistically interfere with complex disorders. In this study we describe the safe application of ethanolic chamomile (Matricaria recutita flowers extract (CFE for the treatment and prevention of type 2 diabetes and associated disorders. We show in vitro that this extract activates in particular nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ and its isotypes. In a cellular context, in human primary adipocytes CFE administration (300 µg/ml led to specific expression of target genes of PPARγ, whereas in human hepatocytes CFE-induced we detected expression changes of genes that were regulated by PPARα. In vivo treatment of insulin-resistant high-fat diet (HFD-fed C57BL/6 mice with CFE (200 mg/kg/d for 6 weeks considerably reduced insulin resistance, glucose intolerance, plasma triacylglycerol, non-esterified fatty acids (NEFA and LDL/VLDL cholesterol. Co-feeding of lean C57BL/6 mice a HFD with 200 mg/kg/d CFE for 20 weeks showed effective prevention of fatty liver formation and hepatic inflammation, indicating additionally hepatoprotective effects of the extract. Moreover, CFE treatment did not reveal side effects, which have otherwise been associated with strong synthetic PPAR-targeting molecules, such as weight gain, liver disorders, hemodilution or bone cell turnover. Taken together, modulation of PPARs and other factors by chamomile flowers extract has the potential to prevent or treat type 2 diabetes and related disorders.

  11. Antidiabetic effects of chamomile flowers extract in obese mice through transcriptional stimulation of nutrient sensors of the peroxisome proliferator-activated receptor (PPAR) family.

    Science.gov (United States)

    Weidner, Christopher; Wowro, Sylvia J; Rousseau, Morten; Freiwald, Anja; Kodelja, Vitam; Abdel-Aziz, Heba; Kelber, Olaf; Sauer, Sascha

    2013-01-01

    Given the significant increases in the incidence of metabolic diseases, efficient strategies for preventing and treating of these common disorders are urgently needed. This includes the development of phytopharmaceutical products or functional foods to prevent or cure metabolic diseases. Plant extracts from edible biomaterial provide a potential resource of structurally diverse molecules that can synergistically interfere with complex disorders. In this study we describe the safe application of ethanolic chamomile (Matricaria recutita) flowers extract (CFE) for the treatment and prevention of type 2 diabetes and associated disorders. We show in vitro that this extract activates in particular nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) and its isotypes. In a cellular context, in human primary adipocytes CFE administration (300 µg/ml) led to specific expression of target genes of PPARγ, whereas in human hepatocytes CFE-induced we detected expression changes of genes that were regulated by PPARα. In vivo treatment of insulin-resistant high-fat diet (HFD)-fed C57BL/6 mice with CFE (200 mg/kg/d) for 6 weeks considerably reduced insulin resistance, glucose intolerance, plasma triacylglycerol, non-esterified fatty acids (NEFA) and LDL/VLDL cholesterol. Co-feeding of lean C57BL/6 mice a HFD with 200 mg/kg/d CFE for 20 weeks showed effective prevention of fatty liver formation and hepatic inflammation, indicating additionally hepatoprotective effects of the extract. Moreover, CFE treatment did not reveal side effects, which have otherwise been associated with strong synthetic PPAR-targeting molecules, such as weight gain, liver disorders, hemodilution or bone cell turnover. Taken together, modulation of PPARs and other factors by chamomile flowers extract has the potential to prevent or treat type 2 diabetes and related disorders.

  12. Dye-sensitized solar cells using natural dye as light-harvesting materials extracted from Acanthus sennii chiovenda flower and Euphorbia cotinifolia leaf

    Directory of Open Access Journals (Sweden)

    Wuletaw Andargie Ayalew

    2016-12-01

    Full Text Available Natural dyes are environmentally and economically superior to ruthenium-based dyes because they are nontoxic and cheap. In this study, dye-sensitized solar cells (DSSCs were fabricated using natural dyes light harvesting materials. The natural dyes were extracted from Acanthus sennii chiovenda flower and Euphorbia cotinifolia leaf. In the as-prepared DSSC, a quasi-solid state electrolyte was sandwiched between the working electrode (photoanode and counter electrode (PEDOT-coated FTO glass. The photoelectrochemical performance of the as-prepared quasi-solid state DSSCs showed open-circuit voltages (VOC varied from 0.475 to 0.507 V, the short-circuit current densities (JSC ranged from 0.352 to 0.642 mA cm−2 and the fill factors (FF varied from 47 to 60% at 100 mWcm−2 light intensity. The dye extracted from A. sennii chiovenda flower, using acidified ethanol (in 1% HCl as extracting solvent, exhibited best conversion efficiency with a maximum open-circuit voltage (VOC of 0.507 V, short-circuit current density (JSC of 0.491 mA cm−2, fill factor (FF of 0.60 and an overall conversion efficiency (η of 0.15%. On the other hand, the maximum power conversion efficiency of the dye extracted from E. cotinifolia leaf was 0.136%. This is the first study that reports the fabrication of DSSC using natural dye sensitizers extracted from these plants in the presence of quasi-solid state electrolyte and PEDOT as a counter electrode.

  13. Effect of Aloe Barbadensis on Rat's Uterine Contractility | Iranloye ...

    African Journals Online (AJOL)

    The present study was undertaken to investigate the effect of the aqueous extract of Aloe barbadensis on the contractility of the uterine stip of a rat. Aqueous extract at final bath concentrations (FBC)1x10-4 mg/ml to 3x101 mg/ml produced a progressive increase in frequency of contraction of the uterine strip. The force of ...

  14. INDIKATOR TITRASI ASAM-BASA DARI EKSTRAK BUNGA SEPATU (Hibiscus rosa sinensis L Indicator of Acid-Base Titration from the Extract of Hibiscus rosa sinensis L Flower

    Directory of Open Access Journals (Sweden)

    Siti Nuryanti

    2012-05-01

    Full Text Available Titration acid-base needs indicator  to show the change of color on interval of hydrogen exponent/degree of acid (pH. Indicator of synthetic which always be used have disadvantages like chemical pollution,  stock and expensive of pro- duction  cost. The research has been carried out to substitute the synthetic indicator with herbal indicator extracted from flower crown of Hibiscus rosa sinensis L extract. The herbal indicator was extracted from the flower crown Hibiscus rosa sinensis L using a mixture methanol-acetic acid. Then it was evaluated with phenolphthalein and methyl orange(E merck comparer to titration the acid-base, they are strong acid-strong base, weak base-strong acid and weak acid-strong base. The result of research show that herbal indicator  of flower crown Hibiscus rosa sinensis L to show theequivalent point in all titrations give peer result with the comparison. With the research result hoped that indicatortitration acid-base flower crown Hibiscus rosa sinensis L is able to as replace synthetic indicator  (metyl orange andphenolphtalein which always be used before. ABSTRAK Titrasi asam-basa memerlukan indikator untuk menunjukkan perubahan warna pada setiap interval derajad keasaman (pH. Indikator sintetis yang digunakan selama ini mempunyai beberapa kelemahan seperti polusi kimia, ketersediaan dan biaya produksi mahal. Upaya penelitian sudah dilakukan untuk menggantikan indikator sintetis dengan indikator dari ekstrak mahkota bunga sepatu. Indikator herbal tersebut dibuat dengan cara mengekstrak mahkota bunga Hibiscus rosa sinensis L dengan mengunakan pelarut metanol-asam asetat. Kemudian dievaluasi dengan indikator pembanding fenolftalein dan metil oranye (produksi E merck untuk titrasi asam-basa yaitu asam kuat-basa kuat, basa lemah-asam kuat dan asam lemah-basa kuat. Dari hasil penelitian diketahui bahwa indikator dari mahkota bunga sepatu untuk menunjukkan titik ekivalen dalam titrasi tersebut memberikan hasil yang

  15. Cytotoxicity and Proapoptotic Effects of Allium atroviolaceum Flower Extract by Modulating Cell Cycle Arrest and Caspase-Dependent and p53-Independent Pathway in Breast Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Somayeh Khazaei

    2017-01-01

    Full Text Available Breast cancer is the second leading cause of cancer death among women and despite significant advances in therapy, it remains a critical health problem worldwide. Allium atroviolaceum is an herbaceous plant, with limited information about the therapeutic capability. We aimed to study the anticancer effect of flower extract and the mechanisms of action in MCF-7 and MDA-MB-231. The extract inhibits the proliferation of the cells in a time- and dose-dependent manner. The underlying mechanism involved the stimulation of S and G2/M phase arrest in MCF-7 and S phase arrest in MDA-MB-231 associated with decreased level of Cdk1, in a p53-independent pathway. Furthermore, the extract induces apoptosis in both cell lines, as indicated by the percentage of sub-G0 population, the morphological changes observed by phase contrast and fluorescent microscopy, and increase in Annexin-V-positive cells. The apoptosis induction was related to downregulation of Bcl-2 and also likely to be caspase-dependent. Moreover, the combination of the extract and tamoxifen exhibits synergistic effect, suggesting that it can complement current chemotherapy. LC-MS analysis displayed 17 major compounds in the extract which might be responsible for the observed effects. Overall, this study demonstrates the potential applications of Allium atroviolaceum extract as an anticancer drug for breast cancer treatment.

  16. Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

    Directory of Open Access Journals (Sweden)

    İrem Atay Balkan

    2018-03-01

    Full Text Available The in vitro anti-inflammatory activity profile of the Nerium oleander flower EtOH extract/its subextracts (n-hexane, CH 2Cl 2, EtOAc, remaining H 2O were evaluated on LPS induced Raw 264.7 macrophages. The effects of the crude EtOH extract and its subextracts on nitric oxide (NO production and cell viability were determined. The most active subextract was determined to be the EtOAc subextract without exerting any toxicity towards Raw 264.7 macrophages. This subextract significantly inhibited NO production of Raw 264.7 macrophages after LPS induction (62.56±1.91% at 200 µg/mL concentration. The levels of iNOS were reduced up to 67.50%. Moreover, this subextract slightly reduced the phosphorylation levels of MAP kinases (p-ERK, p-JNK, p-38. The highest inhibition was observed for ERK phosphorylation, which was inhibited by 20.53% at 200 µg/mL concentration. Through activity-guided fractionation procedures, kaempferol, kaempferol 3-O-β-glucopyranoside and chlorogenic acid were isolated as the main active components. The structures of the active compounds were determined by 2D-NMR techniques and HRMS analysis. All compounds significantly inhibited NO productions. Results of the present study supported the traditional use of N. oleander flowers to treat inflammatory complaints.

  17. Green Synthesis of Silver Nanoparticles using Achillea biebersteinii Flower Extract and Its Anti-Angiogenic Properties in the Rat Aortic Ring Model

    Directory of Open Access Journals (Sweden)

    Javad Baharara

    2014-04-01

    Full Text Available Silver nanoparticles display unique physical and biological properties which have attracted intensive research interest because of their important medical applications. In this study silver nanoparticles (Ab.Ag-NPs were synthesized for biomedical applications using a completely green biosynthetic method using Achillea biebersteinii flowers extract. The structure and properties of Ab.Ag-NPs were investigated using UV-visible spectroscopic techniques, transmission electron microscopy (TEM, zeta potential and energy dispersive X-ray spectrometers (EDS. The UV-visible spectroscopic analysis showed the absorbance peak at 460 nm, which indicates the synthesis of silver nanoparticles. The average particle diameter as determined by TEM was found to be 12 ± 2 nm. The zeta potential analysis indicated that Ab.Ag-NPs have good stability EDX analysis also exhibits presentation of silver element. As angiogenesis is an important phenomenon and as growth factors imbalance in this process causes the acceleration of several diseases including cancer, the anti-angiogenic properties of Ab.Ag-NPs were evaluated using the rat aortic ring model. The results showed that Ab.Ag-NPs (200 μg/mL lead to a 50% reduction in the length and number of vessel-like structures. The synthesized silver nanoparticles from the Achillea biebersteinii flowers extract, which do not involve any harmful chemicals were well-dispersed and stabilized through this green method and showed potential therapeutic benefits against angiogenesis.

  18. Green synthesis and characterization of silver nanoparticles using alcoholic flower extract of Nyctanthes arbortristis and in vitro investigation of their antibacterial and cytotoxic activities

    Energy Technology Data Exchange (ETDEWEB)

    Gogoi, Nayanmoni [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India); Babu, Punuri Jayasekhar [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India); Mahanta, Chandan [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India); Bora, Utpal, E-mail: ubora@iitg.ernet.in [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India)

    2015-01-01

    Here we report the synthesis of silver nanoparticles using ethanolic flower extract of Nyctanthes arbortristis, UVvisible spectra and TEM indicated the successful formation of silver nanoparticles. Crystalline nature of the silver nanoparticles was confirmed by X-ray diffraction. Fourier Transform Infra-Red Spectroscopy analysis established the capping of the synthesized silver nanoparticles with phytochemicals naturally occurring in the ethanolic flower extract of N. arbortristis. The synthesized silver nanoparticles showed antibacterial activity against the pathogenic strain of Escherichia coli MTCC 443. Furthermore, cytotoxicity of the silver nanoparticles was tested on mouse fibroblastic cell line (L929) and found to be non-toxic, which thus proved their biocompatibility. Antibacterial activity and cytotoxicity assay carried out in this study open up an important perspective of the synthesized silver nanoparticles. - Highlights: • The present study depicts the green synthesis of AgNPs using Nyctanthes arbortristis. • AuNPs found to be biocompatible and can be used for biomedical applications. • The FTIR, TGA and DTA results showed that AgNPs are bounded by organic coating. • The synthesized AgNPs showed antibacterial activity on E. Coli MTCC 443. • We investigated the antioxidant activity for both EFE and AgNPs.

  19. Flowering Trees

    Indian Academy of Sciences (India)

    narrow towards base. Flowers are large and attrac- tive, but emit unpleasant foetid smell. They appear in small numbers on erect terminal clusters and open at night. Stamens are numerous, pink or white. Style is slender and long, terminating in a small stigma. Fruit is green, ovoid and indistinctly lobed. Flowering Trees.

  20. Flowering Trees

    Indian Academy of Sciences (India)

    Flowering Trees. Acrocarpus fraxinifolius Wight & Arn. (PINK CEDAR, AUSTRALIAN ASH) of. Caesalpiniaceae is a lofty unarmed deciduous native tree that attains a height of 30–60m with buttresses. Bark is thin and light grey. Leaves are compound and bright red when young. Flowers in dense, erect, axillary racemes.

  1. Flowering Trees

    Indian Academy of Sciences (India)

    Srimath

    quick-growing deciduous tree with a small crown. Branches are covered with dark conical prickles, which fall off after some time. The leaves are compound with three leaflets. Bright red or scarlet flowers which appear following leaf fall are in clusters at branch ends. Birds and bees visit flowers for nectar. Fruit is a cylindrical ...

  2. Flowering Trees

    Indian Academy of Sciences (India)

    conspicuous spines. Leaves are simple, in pairs at each node and are shiny. Inflorescence is an axillary few-flowered cymose fascicle. Flowers are small (less than 1 cm across), 4-merous and greenish-white. Fruit is ellipsoid, 115cm and is edible when ripe. C. parviflorum is one of the commonest species of the scrub jungle ...

  3. Flowering Trees

    Indian Academy of Sciences (India)

    Brachichiton acerifolius F. Muell., commonly called as the Illawara flame tree is a member of Malvaceae family and is native to sub-tropical parts of Australia. Due to its spectacular flowers and tolerance to wide range of climates, it's now cultivated all over the world for its beauty. The tree produces flowers during the.

  4. Flowering Trees

    Indian Academy of Sciences (India)

    Canthium parviflorum Lam. of Rubiaceae is a large shrub that often grows into a small tree with conspicuous spines. Leaves are simple, in pairs at each node and are shiny. Inflorescence is an axillary few-flowered cymose fascicle. Flowers are small (less than 1 cm across), 4-merous and greenish-white. Fruit is ellipsoid ...

  5. The increasing of fibroblast growth factor 2, osteocalcin, and osteoblast due to the induction of the combination of Aloe vera and 2% xenograft concelous bovine

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2012-12-01

    Full Text Available Background: To make a successfull denture prominent ridge is needed, preservation on tooth extraction socket is needed in order to prevent alveol bone resorption caused by revocation trauma. An innovative modification of the material empirically suspected to be able reduce inflammation caused by the revocation trauma is a combination of Aloe vera and xenograft concelous bovine (XCB and Aloe vera is a biogenic stimulator and accelerating the growth of alveolar ridge bone after tooth extraction. Purpose: The research was aimed to determine of the increasing alveol bone formation by inducing the combination of Aloe vera and 2% xenograft concelous bovine. Methods: To address the problems, the combination of Aloe vera and xenograft concelous bovine was induced into the tooth extraction sockets of Cavia cabayas which devided on 8 groups. Groups control, filled with XCB, Aloe vera and Aloe vera and XCB combination, at 7 days and 30 days after extraction. Afterwards, immunohistochemical examination was conducted to examine the expressions of FGF-2 and osteocalcin, as the product of the growth of osteoblasts. Results: There were significantly increases expression of FGF-2 and osteocalcyn on group which filled with XCB, Aloe vera and combined Aloe vera and XCB. Conclusion: It may be concluded that the induction of the combination of Aloe vera and xenograft concelous bovine into the tooth sockets can enhance the growth expressions of FGF-2 and osteocalcin as the product of osteoblasts, thus, the growth of alveolar bone was increased.Latar belakang: Untuk keberhasilan pembuatan gigitiruan diperlukan ridge yang prominent, maka diperlukan suatu preservasi soket pencabutan gigi untuk mencegah terjadinya resopsi tulang alveolar akibat trauma pencabutan. Suatu inovasi modifikasi bahan yang diduga secara empiris dapat mengurangi keradangan karena trauma pencabutan adalah berupa kombinasi Aloe vera dan xenograft concelous bovine (XCB. Aloe vera yang merupakan

  6. Compatibility assessment between four ethanolic plant extracts with a bug predator Orius horvathi (Reuter (Heteroptera: Anthocoridae used for controlling the western flower thrips Frankliniella occidentalis (Pergande (Thysanoptera: Thripidae

    Directory of Open Access Journals (Sweden)

    Razavi Nooshin

    2016-01-01

    Full Text Available The western flower thrips, Frankliniella occidentalis (Pergande attacks a large number of crop plants. The current insecticides have caused resistance in insects and have caused outbreaks of thrips. In many instances, alternative methods of insect management and natural products, offer adequate pest control and pose fewer hazards. Several species of minute pirate bugs of the genus Orius play a significant role in the biological control of a large number of thrips species, such as F. occidentalis. In this study, the insecticidal activity of four ethanolic plant extracts (Cercis siliquastrum L., Calendula officinalis L., Peganum harmala L., Melia azedarach L. in integration with Orius horvathi (Reuter were evaluated for controlling F. occidentalis. The present research aimed to find plant extracts with a good impact on F. occidentalis but which have fewer side effects on O. horvathi. The results showed that P. harmala extract can be considered compatible with the natural enemy for controlling thrips. When the predatory bugs O. horvathi, were released three days after P. harmala extract spraying, the integration was more effective. While the P. harmala plant extract plays an important role in thrips control, it is necessary to consider the specified time interval between the application of the P. harmala plant extract and the release of the O. horvathi predatory bugs. The ethanolic extract of M. azedarach caused a balance between the pest population and the natural enemy. This result is very important in an Integrated Pest Management (IPM program because this ethanolic extract of M. azedarach had lower side effects on the natural enemy. This means that an integration of plant derived chemicals and the natural enemy, O. horvathi, can effectively control thrips.

  7. Aloe in Angola (Asphodelaceae: Alooideae

    Directory of Open Access Journals (Sweden)

    R. R. Klopper

    2009-08-01

    Full Text Available Botanical exploration of Angola was virtually impossible during the almost three-decade-long civil war. With more areas becoming accessible, there is, however, a revived interest in the flora of this country. A total of 27 members of the genus Aloe L. have been recorded for Angola. It is not unlikely that new taxa will be discovered, and that the distribution ranges of others will be expanded now that botanical exploration in Angola has resumed. This manuscript provides a complete taxonomic treatment of the known Aloe taxa in Angola. It includes, amongst other information, identification keys, descriptions and distribution maps.

  8. Effect of Aloe barbadensis Mill. formulation on Letrozole induced polycystic ovarian syndrome rat model

    Directory of Open Access Journals (Sweden)

    Radha Maharjan

    2010-01-01

    Full Text Available This is a preliminary study that explores the efficacy of Aloe vera gel formulation as a possible therapeutic agent in the prevention and management of polycystic ovary syndrome (PCOS. PCOS is recognized as the most common endocrinopathy of women. Increased androgen synthesis, disrupted folliculogenesis, and insulin resistance lie at the patho-physiological core of PCOS. Current therapy for such a syndrome is use of insulin sensitizers. Large randomized clinical trials of metformin as the insulin-sensitizing drug, however, suggested that it produces many side effects after prolonged usage. For this reason, an alternate therapy would be to use herbs with hypoglycemic potential. Aloe barbadensis Mill. (Liliaceae popularly known as Aloe vera is a well-known plant with such properties. The present study evaluated the efficacy of Aloe vera gel formulation in a PCOS rat model. Five month old Charles Foster female rats were orally fed with letrozole, a non-steroidal aromatase inhibitor, to induce PCOS. The rats were then treated orally with the Aloe vera gel formulation (1 ml dose daily for 45 days. This restored their estrus cyclicity, glucose sensitivity, and steroidogenic activity. Co-treatment of the inductive agent (letrozole with the Aloe vera gel prevented the development of the PCO phenotype. Aloe vera gel formulation exerts a protective effect in against the PCOS phenotype by restoring the ovarian steroid status, and altering key steroidogenic activity. This can be attributed to phyto-components present in the extract.

  9. Factorial design of essential oil extraction from Fagraea fragrans Roxb. flowers and evaluation of its biological activities for perfumery and cosmetic applications.

    Science.gov (United States)

    Yingngam, B; Brantner, A H

    2015-06-01

    To optimize the extraction yields of essential oil from Fagraea fragrans Roxb. flowers in hydro-distillation using a central composite design (CCD) and to evaluate its biological activities for perfumery and cosmetic applications. Central composite design was applied to study the influences of operational parameters [water to flower weight (X(1)) and distillation time (X(2))] on the yields of essential oil (Y). Chemical compositions of the essential oil extracted from the optimized condition were identified by gas chromatography-mass spectrometry. Antioxidant activities of the essential oil were determined against ABTS(•+) and DPPH(•) radicals, and the cytotoxic effects were assessed on human embryonic kidney (HEK293) cells by the use of the MTT assay. Also, the aromatic properties of the essential oil were evaluated by five healthy trained volunteers. The best conditions to obtain the maximum essential oil yield were 7.5 mL g(-1) (X(1)) and 215 min (X(2)). The experimental yield of the essential oil (0.35 ± 0.02% v/w) was close to the value predicted by a mathematical model (0.35 ± 0.01% v/w). 3-Octadecyne, Z,Z,Z-7,10,13-hexadecatrienal, E-nerolidol, pentadecanal and linalool were the major constituents of the essential oil. The essential oil showed moderate antioxidant capacities with no toxic effects on HEK293 cells at 1-250 μg mL(-1). Also, the essential oil exhibited a very strong aroma and was classified to be top- to middle-notes. The results offer the effectively operational conditions in the extraction of essential oil from F. fragrans using hydro-distillation. The essential oil could be used as a natural fragrance, having antioxidant activity with slight cytotoxicity, for perfumery and cosmetic applications. © 2014 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

  10. Down-Regulation of Glycosyl Transferase Genes in Streptococcus Mutans by Punica Granatum L. Flower and Rhus Coriaria L. Fruit Water Extracts

    Science.gov (United States)

    Vahid-Dastjerdi, Elahe; Monadi, Elham; Khalighi, Hamid Reza; Torshabi, Maryam

    2016-01-01

    In our previous studies, we showed the inhibitory effects of Punica granatum L. flower and Rhus coriaria L. fruit water extracts on dental plaque accumulation by several bacteria, especially Streptococcus mutans (S. mutans), on orthodontic wire by in-vitro assays. In this study, the anti-cariogenic properties of the extracts were evaluated by assessing their effects on expression of glycosyltransferase (gtf) genes, which are responsible for initial biofilm formation by S. mutans. In this study, the effect of herbal extracts on expression of gtfB, C (encoding enzymes that produce water-insoluble glucans) and D (encoding enzymes that produce water-soluble glucans) genes in S. mutans growing in planktonic state was evaluated quantitatively by real-time polymerase chain reaction (PCR) method. The minimum biofilm inhibitory concentration (MBIC) of understudied herbal water extracts significantly suppressed gtfB, C and D gene expression by 85.3 ± 7.5%, 33.3 ± 6.4% and 25 ± 14%, respectively for Punica granatum L. extract and 73.4 ± 7.3%, 93.8 ± 2.7% and 59.3 ± 9.8%, respectively for Rhus coriaria L. extract compared to the non-treated control group (P Punica granatum L. extract. These findings suggest that Punica granatum L. and especially Rhus coriaria L. maybe used as novel, natural antiplaque agents since they inhibit specific genes associated with bacterial biofilm formation without necessarily affecting the growth of oral bacteria. PMID:27642322

  11. Down-Regulation of Glycosyl Transferase Genes in Streptococcus Mutans by Punica Granatum L. Flower and Rhus Coriaria L. Fruit Water Extracts.

    Science.gov (United States)

    Vahid-Dastjerdi, Elahe; Monadi, Elham; Khalighi, Hamid Reza; Torshabi, Maryam

    2016-01-01

    In our previous studies, we showed the inhibitory effects of Punica granatum L. flower and Rhus coriaria L. fruit water extracts on dental plaque accumulation by several bacteria, especially Streptococcus mutans (S. mutans), on orthodontic wire by in-vitro assays. In this study, the anti-cariogenic properties of the extracts were evaluated by assessing their effects on expression of glycosyltransferase (gtf) genes, which are responsible for initial biofilm formation by S. mutans. In this study, the effect of herbal extracts on expression of gtfB, C (encoding enzymes that produce water-insoluble glucans) and D (encoding enzymes that produce water-soluble glucans) genes in S. mutans growing in planktonic state was evaluated quantitatively by real-time polymerase chain reaction (PCR) method. The minimum biofilm inhibitory concentration (MBIC) of understudied herbal water extracts significantly suppressed gtfB, C and D gene expression by 85.3 ± 7.5%, 33.3 ± 6.4% and 25 ± 14%, respectively for Punica granatum L. extract and 73.4 ± 7.3%, 93.8 ± 2.7% and 59.3 ± 9.8%, respectively for Rhus coriaria L. extract compared to the non-treated control group (P Punica granatum L. extract. These findings suggest that Punica granatum L. and especially Rhus coriaria L. maybe used as novel, natural antiplaque agents since they inhibit specific genes associated with bacterial biofilm formation without necessarily affecting the growth of oral bacteria.

  12. Application of the threshold of toxicological concern approach for the safety evaluation of calendula flower (Calendula officinalis) petals and extracts used in cosmetic and personal care products.

    Science.gov (United States)

    Re, T A; Mooney, D; Antignac, E; Dufour, E; Bark, I; Srinivasan, V; Nohynek, G

    2009-06-01

    Calendula flower (Calendula officinalis) (CF) has been used in herbal medicine because of its anti-inflammatory activity. CF and C. officinalis extracts (CFE) are used as skin conditioning agents in cosmetics. Although data on dermal irritation and sensitization of CF and CFE's are available, the risk of subchronic systemic toxicity following dermal application has not been evaluated. The threshold of toxicological concern (TTC) is a pragmatic, risk assessment based approach that has gained regulatory acceptance for food and has been recently adapted to address cosmetic ingredient safety. The purpose of this paper is to determine if the safe use of CF and CFE can be established based upon the TTC class for each of its known constituents. For each constituent, the concentration in the plant, the molecular weight, and the estimated skin penetration potential were used to calculate a maximal daily systemic exposure which was then compared to its corresponding TTC class value. Since the composition of plant extracts are variable, back calculation was used to determine the maximum acceptable concentration of a given constituent in an extract of CF. This paper demonstrates the utility and practical application of the TTC concept when used as a tool in the safety evaluation of botanical extracts.

  13. A novel molybdenum disulfide nanosheet self-assembled flower-like monolithic sorbent for solid-phase extraction with high efficiency and long service life.

    Science.gov (United States)

    Ran, Fanpeng; Liu, Hongmei; Wang, Xiaoqi; Guo, Yong

    2017-07-21

    A novel material consisting of molybdenum disulfide (MoS 2 ) nanosheet that self-assemble into flower-like microspheres which aggregate to form a monolithic matrix with a micro or nano-scaled mesopore structure was successfully synthesized and used as an efficient sorbent for solid-phase extraction (SPE) due to its large specific adsorption area and good stability. The extraction properties of the as-prepared sorbent were evaluated by high-performance liquid chromatography with variable wavelength detection (HPLC-VWD) by analyzing four flavonoids (apigenin, quercetin, luteolin, and kaempferol). Under optimal conditions, the LODs and LOQs were found to be in the ranges of 0.1-0.25 and 0.4-0.5μgL -1 , respectively, and wide linear ranges were obtained with correlation coefficients (R) ranging from 0.9991 to 0.9996. Compared with commercial C18 and Alumina-N sorbents, the as-prepared sorbent showed high extraction efficiency at different concentrations of flavonoids. After 100 uses, the extraction ability of the self-assembled MoS 2 nanosheet monolithic sorbent had no evident decline, denoting a long service life. Finally, the SPE-HPLC-VWD method using the as-prepared sorbent was applied to flavonoid analysis in beverage samples with satisfactory results. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Tandem solid-phase extraction followed by HPLC-ESI/QTOF/MS/MS for rapid screening and structural identification of trace diterpenoids in flowers of Rhododendron molle.

    Science.gov (United States)

    Zou, Hong-Yan; Luo, Jun; Xu, De-Ran; Kong, Ling-Yi

    2014-01-01

    'Naoyanghua', composed of the flowers of Rhododendron molle G. Don, is a traditional Chinese medicine that is widely known for its toxicity. Grayanane-type diterpenoids are the main active ingredients in R. molle, as well as possibly their toxicity: they are, however, difficult to isolate and analyse using common chromatographic methods, due to their small amounts and absence of conjugated groups, such as phenyl and α, β-unsaturated ketone. To establish a highly sensitive, selective and reliable method for the qualitative evaluation of trace diterpenoids in the flowers of R. molle by using tandem solid-phase extraction followed by high-performance liquid chromatography with electrospray ionisation quadrupole-time-of-flight mass spectrometry (HPLC-ESI/QTOF/MS/MS). Tandem solid phase extraction (SPE) was undertaken using a polyamide cartridge and a C18E cartridge in succession to enrich the trace diterpenoids. HPLC-ESI/QTOF/MS/MS was used to determine the fragmentation patterns of diterpenoids and to tentatively characterise their fragmentation pathways. HPLC-ESI/QTOF/MS/MS detected a total of 14 diterpenoids, eight of which were identified by comparison with literature sources and six based on fragmentation analysis. Among the latter six, rhodojaponin VI-3-glucoside was tentatively identified as a new diterpenoid glycoside and rhodojaponin VII, rhodojaponin IV and rhodojaponin I were reported from R. molle for the first time. By qualitative research of diterpenoids in this plant by HPLC-ESI/QTOF/MS/MS, a reliable methodology for the analysis of these active constituents of R. molle was established for the first time. Copyright © 2014 John Wiley & Sons, Ltd.

  15. Aloe vera for prevention and treatment of infusion phlebitis.

    Science.gov (United States)

    Zheng, Guo Hua; Yang, Liu; Chen, Hai Ying; Chu, Jian Feng; Mei, Lijuan

    2014-06-04

    Up to 80% of hospitalised patients receive intravenous therapy at some point during their admission. About 20% to 70% of patients receiving intravenous therapy develop phlebitis. Infusion phlebitis has become one of the most common complications in patients with intravenous therapy. However, the effects of routine treatments such as external application of 75% alcohol or 50% to 75% magnesium sulphate (MgSO4) are unsatisfactory. Therefore, there is an urgent need to develop new methods to prevent and alleviate infusion phlebitis. To systematically assess the effects of external application of Aloe vera for the prevention and treatment of infusion phlebitis associated with the presence of an intravenous access device. The Cochrane Peripheral Vascular Diseases Group Trials Search Co-ordinator (TSC) searched the Specialised Register (last searched February 2014) and CENTRAL (2014, Issue 1). In addition the TSC searched MEDLINE to week 5 January 2014, EMBASE to Week 6 2014 and AMED to February 2014. The authors searched the following Chinese databases until 28 February 2014: Chinese BioMedical Database; Traditional Chinese Medical Database System; China National Knowledge Infrastructure; Chinese VIP information; Chinese Medical Current Contents; Chinese Academic Conference Papers Database and Chinese Dissertation Database; and China Medical Academic Conference. Bibliographies of retrieved and relevant publications were searched. There were no restrictions on the basis of date or language of publication. Randomised controlled trials (RCTs) and quasi-randomised controlled trials (qRCTs) were included if they involved participants receiving topical Aloe vera or Aloe vera-derived products at the site of punctured skin, with or without routine treatment at the same site. Two review authors independently extracted the data on the study characteristics, description of methodology and outcomes of the eligible trials, and assessed study quality. Data were analysed using RevMan 5

  16. Hibiscus Sabdariffa L. Flowers and Olea Europea L. Leaves Extract-Based Formulation for Hypertension Care: In Vitro Efficacy and Toxicological Profile.

    Science.gov (United States)

    Micucci, Matteo; Angeletti, Andrea; Cont, Massimiliano; Corazza, Ivan; Aldini, Rita; Donadio, Elisa; Chiarini, Alberto; Budriesi, Roberta

    2016-05-01

    Olea europaea L. leaves extract (Oe) and Hybiscus sabdariffa L. flowers extract (Hs) have calcium antagonistic properties. Aim of this work was to study the cardiovascular effects of Pres Phytum(®), a nutraceutical formulation containing a mixture of the two extracts and the excipients, and investigate its possible off-target effects, using in vitro biological assays on guinea pig isolated organs. Cardiovascular effects were assessed using guinea pig atria and aorta. The effects of Pres Phytum on spontaneous gastrointestinal, urinary, and respiratory tracts smooth muscle contractility were evaluated. Pres Phytum exerted a vasorelaxant effect (IC50 = 2.38 mg/mL) and a negative chronotropic effect (IC50 = 1.04 mg/mL) at concentrations lower than those producing smooth muscle spontaneous contractility alterations in the other organs. Compared to Pres Phytum, the mixture did not exert negative inotropic activity, while it maintained a negative chronotropic efficacy (IC50 = 1.04 mg/mL). These experimental data suggest a possible nutraceutical use of this food supplement for the management of preclinical hypertension.

  17. Antioxidant capacity of calendula officinalis flowers extract and prevention of radiation induced oropharyngeal mucositis in patients with head and neck cancers: a randomized controlled clinical study.

    Science.gov (United States)

    Babaee, Neda; Moslemi, Dariush; Khalilpour, Mohammad; Vejdani, Fatemeh; Moghadamnia, Yasaman; Bijani, Ali; Baradaran, Mahmoud; Kazemi, Mohammad Taghi; Khalilpour, Asieh; Pouramir, Mahdi; Moghadamnia, Ali Akbar

    2013-03-07

    This study was designed to determine the effect of Calendula officinalis flowers extract mouthwash as oral gel on radiation-induced oropharyngeal mucositis (OM) in patients with head-and-neck cancer. Forty patients with neck and head cancers under radiotherapy or concurrent chemoradiotherapy protocols were randomly assigned to receive either 2% calendula extract mouthwash or placebo (20 patients in each group). Patients were treated with telecobalt radiotherapy at conventional fractionation (200 cGy/fraction, five fractions weekly, 30-35 fractions within 4-7 weeks). The oropharyngeal mucositis was evaluated by two clinical investigators (a radiation oncologist and a dentist), using the oral mucositis assessment scale (OMAS). Trying to find out the possible mechanism of action of the treatment, total antioxidant, polyphenol and flavonoid contents, and quercetin concentration of the mouth wash were measured. Calendula mouthwash significantly decreased the intensity of OM compared to placebo at week 2 (score: 5.5 vs. 6.8, p = 0.019), week 3 (score: 8.25 vs. 10.95, p Calendula extract gel could be effective on decreasing the intensity of radiotherapy- induced OM during the treatment and antioxidant capacity may be partly responsible for the effect.

  18. A comparative study of baby immature and adult shoots of Aloe vera on UVB-induced skin photoaging in vitro.

    Science.gov (United States)

    Hwang, Eunson; Kim, Su Hyeon; Lee, Sarah; Lee, Choong Hwan; Do, Seon-Gil; Kim, Jinwan; Kim, Sun Yeou

    2013-12-01

    Ultraviolet (UV) irradiation induces photo-damage of the skin, which in turn causes depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase (MMP) expression. The production of type I procollagen is regulated by transforming growth factor-β1 (TGF-β1) expression; the activation of MMP is also correlated with an increase of interleukin-6 (IL-6). Aloe barbadensis M. (Aloe vera) is widely used in cosmetic and pharmaceutical products. In this study, we examined whether baby aloe shoot extract (BAE, immature aloe extract), which is from the one-month-old shoots of Aloe vera, and adult aloe shoot extract (AE), which is from the four-month-old shoots of Aloe vera, have a protective effect on UVB-induced skin photoaging in normal human dermal fibroblasts (NHDFs). The effects of BAE and AE on UVB-induced photoaging were tested by measuring the levels of reactive oxygen species, MMP-1, MMP-3, IL-6, type I procollagen, and TGF-β1 after UVB irradiation. We found that NHDF cells treated with BAE after UVB-irradiation suppressed MMP-1, MMP-3, and IL-6 levels compared to the AE-treated cells. Furthermore, BAE treatment elevated type I procollagen and TGF-β1 levels. Our results suggest that BAE may potentially protect the skin from UVB-induced damage more than AE. Copyright © 2013 John Wiley & Sons, Ltd.

  19. Aloe Vera Effervescent Tablet Formulation: Comparation Study of Acid-Alkali-Aloe Powder and Maltodextrin Concentration

    OpenAIRE

    Kasmawarni, Kasmawarni; Utomo, Pramono Putro

    2010-01-01

    The research on The Application of Effervescent Technology of Aloe vera as Functional Drink has been done. The objectives were to obtain effrvescent tablet prefered by the panelist as well as nutritious as aloe vera-based functional food also to obtain appropriate formula resulting good quality of effervescent tablet. Formulation of effervescent tablet with treatments of maltodextrin concentration were done. The result showed that the best formulas of aloe vera effervescent tablet were aloe v...

  20. Antibacterial activity of aloe emodin and aloin A isolated from Aloe ...

    African Journals Online (AJOL)

    GRACE

    2006-06-02

    Jun 2, 2006 ... Aloe excelsa is one of the larger species of aloes from the family Aloaceae (Glen et al., 1997). As with most. Aloe species (Van Wyk et al., 1997), A. excelsa have been used extensively as a traditional remedy. The use of herbal remedies for treatment of various diseases is still vital to the provision of health ...

  1. Bioassay-guided fractionation of extracts from Easter lily (Lilium longiflorum) flowers reveals unprecedented structural variability of steroidal glycoalkaloids.

    Science.gov (United States)

    Uhlig, Silvio; Hussain, Fozia; Wisløff, Helene

    2014-12-15

    Several Lilium species are nephrotoxic in cats (Felis silvestris catus), among them Easter lilies (Lilium longiflorum). Although clinical trials have been carried out, the causative toxic phytochemicals have not yet been identified. We thus aimed to determine the toxic constituents of Easter lily flowers applying a bioassay-guided approach based on a feline kidney cell line model. The bioassay-guided fractionation traced the observed cytotoxicity to a complex mixture of compounds that were tentatively identified as steroidal glycoalkaloids of the solasodine-type, based on multiple-fragmentation ion trap and high-resolution mass spectrometry. The glycoalkaloids in the active fraction possessed trisaccharide chains, and at least 16 different congeners could be separated using liquid chromatography-mass spectrometry. The two principal compounds were solasodine trisaccharides containing two hexose and one deoxy-hexose unit. In the remaining 14 analogues, one or two of the hydroxyl groups of the second hexose from the aglycone were acetylated. In addition, some of the analogues appeared to be carbonate esters. Esterification of steroidal glycoalkaloids in plants has only been reported once and was in accordance with higher antifungal activity of the acetylated versus the parent congener. Our pilot study shows that esterification of steroidal glycoalkaloids in Lilium species might be common resulting in an array of different analogues with largely unexplored structural variability and bioactivity. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Effect of Punica granatum L. Flower Water Extract on Five Common Oral Bacteria and Bacterial Biofilm Formation on Orthodontic Wire

    Science.gov (United States)

    VAHID DASTJERDI, Elahe; ABDOLAZIMI, Zahra; GHAZANFARIAN, Marzieh; AMDJADI, Parisa; KAMALINEJAD, Mohammad; MAHBOUBI, Arash

    2014-01-01

    Background: Use of herbal extracts and essences as natural antibacterial compounds has become increasingly popular for the control of oral infectious diseases. Therefore, finding natural antimicrobial products with the lowest side effects seems necessary. The present study sought to assess the effect of Punica granatum L. water extract on five oral bacteria and bacterial biofilm formation on orthodontic wire. Methods: Antibacterial property of P. granatum L. water extract was primarily evaluated in brain heart infusion agar medium using well-plate method. The minimum inhibitory concentration and minimum bactericidal concentration were determined by macro-dilution method. The inhibitory effect on orthodontic wire bacterial biofilm formation was evaluated using viable cell count in biofilm medium. At the final phase, samples were fixed and analyzed by Scanning Electron Microscopy. Results: The growth inhibition zone diameter was proportional to the extract concentration. The water extract demonstrated the maximum antibacterial effect on Streptococcus sanguinis ATCC 10556 with a minimum inhibitory concentration of 6.25 mg/ml and maximum bactericidal effect on S. sanguinis ATCC 10556 and S. sobrinus ATCC 27607 with minimum bactericidal concentration of 25 mg/ml. The water extract decreased bacterial biofilm formation by S. sanguinis, S. sobrinus, S. salivarius, S. mutans ATCC 35608 and E. faecalis CIP 55142 by 93.7–100%, 40.6–99.9%, 85.2–86.5%, 66.4–84.4% and 35.5–56.3% respectively. Conclusion: Punica granatum L. water extract had significant antibacterial properties against 5 oral bacteria and prevented orthodontic wire bacterial biofilm formation. However, further investigations are required to generalize these results to the clinical setting. PMID:26171362

  3. Effect of Punica granatum L. Flower Water Extract on Five Common Oral Bacteria and Bacterial Biofilm Formation on Orthodontic Wire.

    Science.gov (United States)

    Vahid Dastjerdi, Elahe; Abdolazimi, Zahra; Ghazanfarian, Marzieh; Amdjadi, Parisa; Kamalinejad, Mohammad; Mahboubi, Arash

    2014-12-01

    Use of herbal extracts and essences as natural antibacterial compounds has become increasingly popular for the control of oral infectious diseases. Therefore, finding natural antimicrobial products with the lowest side effects seems necessary. The present study sought to assess the effect of Punica granatum L. water extract on five oral bacteria and bacterial biofilm formation on orthodontic wire. Antibacterial property of P. granatum L. water extract was primarily evaluated in brain heart infusion agar medium using well-plate method. The minimum inhibitory concentration and minimum bactericidal concentration were determined by macro-dilution method. The inhibitory effect on orthodontic wire bacterial biofilm formation was evaluated using viable cell count in biofilm medium. At the final phase, samples were fixed and analyzed by Scanning Electron Microscopy. The growth inhibition zone diameter was proportional to the extract concentration. The water extract demonstrated the maximum antibacterial effect on Streptococcus sanguinis ATCC 10556 with a minimum inhibitory concentration of 6.25 mg/ml and maximum bactericidal effect on S. sanguinis ATCC 10556 and S. sobrinus ATCC 27607 with minimum bactericidal concentration of 25 mg/ml. The water extract decreased bacterial biofilm formation by S. sanguinis, S. sobrinus, S. salivarius, S. mutans ATCC 35608 and E. faecalis CIP 55142 by 93.7-100%, 40.6-99.9%, 85.2-86.5%, 66.4-84.4% and 35.5-56.3% respectively. Punica granatum L. water extract had significant antibacterial properties against 5 oral bacteria and prevented orthodontic wire bacterial biofilm formation. However, further investigations are required to generalize these results to the clinical setting.

  4. Antioxidant Capacity of Calendula Officinalis Flowers Extract and Prevention of Radiation Induced Oropharyngeal Mucositis in Patients with Head and Neck Cancers: A Randomized Controlled Clinical Study

    Directory of Open Access Journals (Sweden)

    Mohammad Taghi Kazemi

    2013-03-01

    Full Text Available This study was designed to determine the effect of Calendula officinalis flowers extract mouthwash as oral gel on radiation-induced oropharyngeal mucositis (OM in patients with head-and-neck cancer. Forty patients with neck and head cancers under radiotherapy or concurrent chemoradiotherapy protocols were randomly assigned to receive either 2% calendula extract mouthwash or placebo (20 patients in each group. Patients were treated with telecobalt radiotherapy at conventional fractionation (200 cGy/fraction, five fractions weekly, 30–35 fractions within 4–7 weeks. The oropharyngeal mucositis was evaluated by two clinical investigators (a radiation oncologist and a dentist, using the oral mucositis assessment scale (OMAS. Trying to find out the possible mechanism of action of the treatment, total antioxidant, polyphenol and flavonoid contents, and quercetin concentration of the mouth wash were measured. Calendula mouthwash significantly decreased the intensity of OM compared to placebo at week 2 (score: 5.5 vs. 6.8, p = 0.019, week 3 (score: 8.25 vs. 10.95, p < 0.0001 and week 6 (score: 11.4 vs. 13.35, p = 0.031. Total antioxidant, polyphenol and flavonoid contents and quercetin concentration of the 2% extract were 2353.4 ± 56.5 μM, 313.40 ± 6.52 mg/g, 76.66 ± 23.24 mg/g, and 19.41 ± 4.34 mg/l, respectively. Calendula extract gel could be effective on decreasing the intensity of radiotherapy- induced OM during the treatment and antioxidant capacity may be partly responsible for the effect.

  5. Antiviral activity of Aloe vera against herpes simplex virus type 2: An ...

    African Journals Online (AJOL)

    In this study we tested the antiviral activity of a crude hot glycerine extract of Aloe vera gel which was grown in Bushehr (Southwest of Iran) against HSV-2 replication in Vero cell line. The extract showed antiviral activity against HSV-2 not only before attachment and entry of virus to the Vero cells but also on post attachment ...

  6. Atributos biológicos y terapéuticos de la Sábila (Aloe vera | Biological and therapeutic attributes of Aloe (Aloe vera

    Directory of Open Access Journals (Sweden)

    José Imery Buzia

    2017-11-01

    Full Text Available Aloe, Aloe vera (L. Burm. f. (= A. barbadensis Miller is a monocotyledon, perennial, and succulent plant of the family Asphodelaceae, recognized worldwide for its medicinal properties and ornamental value. For the beauty of their vegetative structures, drought tolerance, popular beliefs and mysticism, Aloe has an important place in the house porches, gardens, terraces, and balconies of many Latin American households. Its healing potential has been supported by multiple laboratories, natural medicine and traditional hospitals that evaluate alternatives for healing of gastrointestinal, epidermal, cardiovascular, respiratory, metabolic, cancer, among other, which are reported to improve the rational management of extracts as a specific treatment to relieve some diseases or as an adjunct to the effects of formal medicine. This work presents a compendium of botanical studies by our research laboratory on local populations of A. vera and provide an update review on its taxonomy, phylogeny, components, and therapeutic uses. Specific anatomical details and morphological traits, evaluated under ambient conditions of tropical very dry forest of northeastern Venezuela, were described and scientific arguments about its origin, evolution and nomenclature were analyzed. This is a contribution to the comprehensive knowledge of this species, whose cultivation and agro-industrial use represents a possibility to improve the quality of life of coastal communities, diversification of local economies and the export of non-traditional products.

  7. Cytotoxic and bioactive properties of different color tulip flowers and degradation kinetic of tulip flower anthocyanins.

    Science.gov (United States)

    Sagdic, Osman; Ekici, Lutfiye; Ozturk, Ismet; Tekinay, Turgay; Polat, Busra; Tastemur, Bilge; Bayram, Okan; Senturk, Berna

    2013-08-01

    This study was conducted to determine the potential use of anthocyanin-based extracts (ABEs) of wasted tulip flowers as food/drug colorants. For this aim, wasted tulip flowers were samples and analyzed for their bioactive properties and cytotoxicity. Total phenolic contents of the extracts of the claret red (126.55 mg of gallic acid equivalent (GAE)/g dry extract) and orange-red (113.76 mg GAE/g dry extract) flowers were the higher than those of the other tulip flowers. Total anthocyanin levels of the violet, orange-red, claret red and pink tulip flower extracts were determined as 265.04, 236.49, 839.08 and 404.45 mg pelargonidin 3-glucoside/kg dry extract, respectively and these levels were higher than those of the other flowers. The extracts were more effective for the inhibition of Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica compared to other tested bacteria. Additionally, the cytotoxic effects of five different tulip flower extracts on human breast adenocarcinoma (MCF-7) cell line were investigated. The results showed that the orange red, pink and violet extracts had no cytotoxic activity against MCF-7 cell lines while yellow and claret red extracts appeared to be toxic for the cells. Overall, the extracts of tulip flowers with different colors possess remarkable bioactive and cytotoxic properties. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Antioxidant activity of alcohol extracts of chamomile flowers, anise seeds and dill seeds in two vegetable oils and two animal fats

    Directory of Open Access Journals (Sweden)

    Al-Ismail, Khalid

    2004-12-01

    Full Text Available The antioxidant activity of alcohol extracts of chamomile flowers (CFE, anise seeds (ASE and dill seeds (DSE in corn oil, soybean oil, beef tallow and anhydrous butter fat (ABF was investigated during storage at 65ºC. The extent of oxidation was followed by peroxide value (PV. In addition, reducing power of these extracts was determined. Alcohol extracts of these plants at 3 g kg-1 were more effective as antioxidant than BHA (0.2 g kg-1 in corn and soybean oils, while they were less effective in beef tallow and ABF. CFE was more effective in retarding fat oxidation in corn and soybean oil than ASE and DSE, which both showed similar activity. However, in beef tallow and ABF all extracts showed similar activities. On the other hand, the reducing power of these extracts showed different behavior. CFE, which was the most active as antioxidant among the extracts showed the lowest reducing power. Furthermore, ASE and DSE, which exhibited similar antioxidant activity, the former has lower reducing activity than the latter, indicating that the antioxidant activity didn’t correlate linearly with the reducing power of these extracts.Se investigó la actividad antioxidante de extractos alcohólicos de flores de camomila (CFE, semillas de anís (ASD y eneldo (DSE en aceites de maíz y soja, sebo y grasa de mantequilla anhidra (ABF, almacenados a 65ºC. El seguimiento de la oxidación se realizó mediante el índice de peróxidos. Además, se determinó el poder reductor de los mismos. La actividad antioxidante de los extractos alcohólicos de estas plantas (3 g k -1 resultó más eficaz que la del BHA (0.2 g kg-1, en cambio, resultaron menos eficaces en el sebo y ABF. El extracto de manzanilla resultó ser mejor antioxidante para los aceites de maíz y soja que los extractos ASE y DSE, ambos mostraron una actividad similar. Sin embargo, en el sebo y la ABF todos los extractos revelaron una actividad antioxidante parecida. El poder reductor de

  9. Stimulation of osteoblast activity by induction of Aloe vera and xenograft combination

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2011-12-01

    Full Text Available Background: Tooth extraction is generally followed by alveolar ridge resorption that later can cause flat ridge. Aloe vera have biogenic stimulator and hormone activities for wound healing. Purpose: This study was aimed to know osteoblast activities in alveolar bone after induction of Aloe vera and XCB combination. Methods: Fifty four of Cavia cabaya were divided into three main groups. Group I was control group. Group II was filled with xenograft concelous bovine (XCB and group III was filled with the combination of Aloe vera gel and XCB. Then, each group was divided into three sub groups according to timing, they are 14, 30, and 60 days after tooth extraction and application. Histology and morphology examination were performed on the harvested specimens. Results: There were significant differences between the control group and the other groups filled with the combination of Aloe vera and XCB. Conclusion: In conclusion, the application of Aloe vera gel and xenograft combination decrease the number of osteoclast and increase the number of osteoblast in post tooth extraction alveolar bone structure indicating the new growth of alveolar bone.Latar belakang: Pencabutan gigi pada umumnya selalu diikuti resopsi tulang alveolar, sehingga bila terjadi dalam waktu yang lama ridge akan menjadi flat. Aloe vera adalah bahan stimulasi biogenik dan mempunyai aktivitas hormon untuk proses penyembuhan luka. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui aktivitas osteoblas pada tulang alveol dengan pemberian kombinasi Aloe vera gel dan xenograft concelous bovine (XCB. Metode: Lima puluh empat ekor Cavia cabaya, dibagi menjadi 3 kelompok besar, kelompok pertama adalah kelompok kontrol yaitu hanya dilakukan pencabutan saja tanpa perlakuan, kelompok ke-2 yaitu kelompok yang setelah dicabut diberi XCB saja dan kelompok ke-3 yaitu kelompok yang setelah pencabutan diberi kombinasi Aloe vera gel dengan XCB pada luka bekas pencabutan gigi. Kemudian masing

  10. Flowering Trees

    Indian Academy of Sciences (India)

    branched evergreen shrub or small tree (6–7 m) with soft whitish-yellow wood. Branches are numerous and drooping. The leaves are elliptic-lanceolate and somewhat fleshy. Flowers are in loose axillary and terminal much-branched inflorescence, ...

  11. Flowering Trees

    Indian Academy of Sciences (India)

    branchlets and grayish-brown bark. Leaves are simple with three to four secondary veins running parallel to the mid vein. Flowers are solitary, male, female and bisexual and inconspicuous. Fruit is berry-like, small and globose with the seed enclosed in a stone. The jurisdiction for all disputes concerning published material, ...

  12. Flowering Trees

    Indian Academy of Sciences (India)

    user

    Tender shoots and the under surface of leaves are covered with dense brown velvety hairs. Flowers are borne on stiff bunches terminally on short shoots. They are 2-3 cm across, white, sweet-scented with light-brown hairy sepals and many stamens. Loquat fruits are round or pear-shaped, 3-5 cm long and are edible.

  13. Flowering Trees

    Indian Academy of Sciences (India)

    deciduous tree with irregularly-shaped trunk, greyish-white scaly bark and milky latex. Leaves in opposite pairs are simple, oblong and whitish beneath. Flowers that occur in branched inflorescence are white, 2–. 3cm across and fragrant. Calyx is glandular inside. Petals bear numerous linear white scales, the corollary.

  14. Flowering Trees

    Indian Academy of Sciences (India)

    Muntingia calabura L. (Singapore cherry) of. Elaeocarpaceae is a medium size handsome ever- green tree. Leaves are simple and alternate with sticky hairs. Flowers are bisexual, bear numerous stamens, white in colour and arise in the leaf axils. Fruit is a berry, edible with several small seeds embedded in a fleshy pulp ...

  15. Flowering Trees

    Indian Academy of Sciences (India)

    Srimath

    . (6-10m high) evergreen tree with a straight trunk and broad open crown. Leaves are clustered at the end of twigs. They are dark green, broadest near the rounded apex and tapering towards the base with a short stalk. Flowers are greenish or ...

  16. Flowering Trees

    Indian Academy of Sciences (India)

    Guaiacum officinale L. (LIGNUM-VITAE) of Zygophyllaceae is a dense-crowned, squat, knobbly, rough and twisted medium-sized ev- ergreen tree with mottled bark. The wood is very hard and resinous. Leaves are compound. The leaflets are smooth, leathery, ovate-ellipti- cal and appear in two pairs. Flowers (about 1.5.

  17. Flowering Trees

    Indian Academy of Sciences (India)

    IAS Admin

    Caesalpinia coriaria (Jacq.) Willd. (THE AMERICAN SUMACH, DIVI-DIVI) of. Caesalpiniaceae is a small unarmed tree reaching up to 10 m in height with a spreading crown. Leaves are alternate and twice compound. The flowers are small, about 0.6 cm (enlarged 5 times here), greenish-yellow, fragrant and appear in dense ...

  18. Flowering Trees

    Indian Academy of Sciences (India)

    Cassia siamia Lamk. (Siamese tree senna) of Caesalpiniaceae is a small or medium size handsome tree. Leaves are alternate, pinnately compound and glandular, upto 18 cm long with 8–12 pairs of leaflets. Inflorescence is axillary or terminal and branched. Flowering lasts for a long period from March to February.

  19. Flowering Trees

    Indian Academy of Sciences (India)

    Srimath

    Grevillea robusta A. Cunn. ex R. Br. (Sil- ver Oak) of Proteaceae is a daintily lacy ornamental tree while young and growing into a mighty tree (45 m). Young shoots are silvery grey and the leaves are fern- like. Flowers are golden-yellow in one- sided racemes (10 cm). Fruit is a boat- shaped, woody follicle.

  20. Flowering Trees

    Indian Academy of Sciences (India)

    Andira inermis (wright) DC. , Dog Almond of Fabaceae is a handsome lofty evergreen tree. Leaves are alternate and pinnately compound with 4–7 pairs of leaflets. Flowers are fragrant and are borne on compact branched inflorescences. Fruit is ellipsoidal one-seeded drupe that is peculiar to members of this family.

  1. Flowering Trees

    Indian Academy of Sciences (India)

    ... shining, greenish-white bark. The leaves are ovate, rarely irregularly lobed, dark green above and pale grey underneath. They are 3-nerved from the base and often crowded at the branch ends. Inflorescence is large, branched with small greenish-yellow flowers which are unpleasant smelling. Fruit is a woody nut with two ...

  2. Flowering Trees

    Indian Academy of Sciences (India)

    Trincomali Wood of Tiliaceae is a tall evergreen tree with straight trunk, smooth brownish-grey bark and simple broad leaves. Inflorescence is much branched with white flowers. Stamens are many with golden yellow anthers. Fruit is a capsule with six spreading wings. Seeds bear short stiff hairs that cause skin irritation.

  3. Flowering Trees

    Indian Academy of Sciences (India)

    stems and handsome foliage. Leaves are 8–10 cm long, dull green, the two thin leathery halves of the lamina fusing or the cleft between them extending beyond the middle. Flowers are gorgeous, axillary with dark purple stamens. The pod is more or less flat. B. alba is often named as B. variegate var. alba by botanists.

  4. Flowering Trees

    Indian Academy of Sciences (India)

    Sterculia foetida L. (INDIAN ALMOND,. JAVA OLIVE) of Sterculiaceae is a tall deciduous tree reaching a height of 20 m with faintly ridged grey bark. The bole reaches up to 2m in girth. Branches are reddish, usually horizontal. Leaves are large, palmately compound (5–7 leaflets) and clustered at the branch ends. Flowers ...

  5. Safety assessment of the aqueous extract of the flowers of Nymphaea lotus Linn (Nymphaeaceae): Acute, neuro- and subchronic oral toxicity studies in albinos Wistar rats.

    Science.gov (United States)

    Kameni Poumeni, Mireille; Bilanda, Danielle Claude; Dzeufiet Djomeni, Paul Désiré; Mengue Ngadena, Yolande Sandrine; Mballa, Marguerite Francine; Ngoungoure, Madeleine Chantal; Ouafo, Agnès Carolle; Dimo, Théophile; Kamtchouing, Pierre

    2017-03-24

    Background Nymphaea lotus Linn (N. lotus) is a medicinal plant widely used in Cameroon popular medicine, to treat neuropsychiatric conditions, male sexual disorders or as food supplement. However, scientific data on the pharmacotoxic profile of this plant are not available. The safety of N. lotus was assessed in acute, neuro- and subchronic toxicity studies by following the OECD guidelines. Effectively, no data have been published until now in regard to its safety on the nervous system. Methods Aqueous extract of N. lotus at doses of 200, 400 and 600 mg/kg body weight (BW) was evaluated for nitrites contents and orally administered to rats daily for 28 days (5 male, 5 female per group). The control group received distilled water (10 mL/kg) and a satellite group was used to observe reversal effects. Neurotoxicity of the plant was determined using open field test for motor coordination, ataxia and gait analysis. Clinical signs and state of livelihood were recorded during the 24 h, then for 28 days of treatments. At the end of 28-day period, animals were anesthetized and decapitated. The whole brain was homogenized for neurobiochemical analysis. Blood samples were collected with or without anticoagulant for hematological examinations and serum analysis. Specimens of liver, kidney, testis, ovaries, and brain were fixed in 10 % formalin and processed for histopathological examinations. Results Our findings indicate dose-dependent elevation of nitrites contents in the flowers aqueous extract of N. lotus. Acute toxicity study revealed no signs of toxicity neither at the dose 2,000 mg/kg nor at 5,000 mg/kg. Thus the LD50 value of aqueous extract of N. lotus flowers is superior to 5,000 mg/kg. The repeated administration of N. lotus during 28 days, induced no signs of neurobehavioral changes in male, but female rats exhibited dose-dependent response in the open field test, suggesting sex and dose-relative psychotropic effects of N. lotus. The evaluation of

  6. A dual and opposite effect of Calendula officinalis flower extract: chemoprotector and promoter in a rat hepatocarcinogenesis model.

    Science.gov (United States)

    Barajas-Farias, L M; Pérez-Carreón, J I; Arce-Popoca, E; Fattel-Fazenda, S; Alemán-Lazarini, L; Hernández-García, S; Salcido-Neyoy, M; Cruz-Jiménez, F G; Camacho, J; Villa-Treviño, S

    2006-02-01

    Calendula officinalis extracts have protective and cytotoxic effects. We previously reported the dual activity of C. officinalis in primary rat hepatocyte cultures treated with N-nitrosodiethylamine. At nM concentrations it was anti-genotoxic while at microM concentrations it exhibited genotoxic effects. Here we tested the activity of Calendula officinalis in vivo in male Fischer 344 rats initiated with N-nitrosodiethylamine, promoted with 2-acetylaminofluorene, and 70 % partially hepatectomized. Liver gamma-glutamyltranspeptidase positively altered hepatocyte foci 25 days after initiation were our end point. The protective effect of C. officinalis started at 0.1 mg/kg concentration, increased at 0.5 mg/kg and reached its maximum at 2.5 mg/kg, when it decreased the area and number of altered foci by 55 % and 49 %, respectively, in comparison with rats treated only with carcinogen. At 5 mg/kg the number and area of altered hepatocyte foci were still lower, but almost reached the figures of carcinogen-treated rats. Ten and 20 mg/kg doses produced a notorious increment in the area and number of altered hepatic foci, and at 40 mg/kg of extract the increment was 40 % and 53 %, respectively. Additionally, when 2-acetylaminofluorene was substituted by a 40 mg/kg C. officinalis extract, a promoting effect was observed with increments of 175 % and 266 % in area and number of altered hepatocyte foci with respect to controls. When N-nitrosodiethylamine was substituted by 40 mg/kg of extract, the latter did not show initiator activity. In summary, we showed a protecting activity of C. officinalis at low doses, but doses above 10 mg/kg increased altered hepatocyte foci. This dual effect is an example of the phenomenon of hormesis. Furthermore, 40 mg/kg of dry weight extract administered instead of 2-acetylaminofluorene induced a clear promoting activity. These in vivo results are similar and consistent with those reported by us in primary rat liver cell cultures.

  7. A specific bioassay for the inhibition of flowering.

    Science.gov (United States)

    Blake, J

    1972-06-01

    A bioassay for the inhibition of flowering involving the in vitro culture of excised, partially-induced, apices of Viscaria candida is described. This bioassay has been used to detect flowering inhibition in extracts from Kalanchoe blossfeldiana.

  8. Non-targeted molecular characterisation of a rose flower ethyl acetate extract using Ultra-HPLC with atmospheric pressure photoionisation and quadrupole time-of-flight MS/MS.

    Science.gov (United States)

    Riffault, Ludivine; Colas, Cyril; Destandau, Emilie; Pasquier, Laure; André, Patrice; Elfakir, Claire

    2015-01-01

    A non-targeted approach to characterise the phytochemical composition of the flower organ of an original rose cultivar 'Jardin de Granville'® was developed. Particular attention was paid to the less documented molecular families of intermediate polarity, compared with the polyphenol family (anthocyanins, flavonoids, tannins) and volatile compounds. To develop a molecular fingerprinting method for the rapid qualitative phytochemical characterisation of the rose flower ethyl acetate extract. An ultra-HPLC with atmospheric pressure photoionisation (APPI) and quadrupole time-of-flight (QTOF) MS/MS combined with microwave-assisted extraction was carried out for ethyl acetate extracts as an intermediate polarity extraction solvent in order to obtain the most exhaustive extract containing a large range of molecular families. An optimised methodology based on the coupling of the UHPLC and APPI source with a QTOF analyser was developed to characterise the extracted molecules. Sixty-one compounds were identified in the extract, covering eight molecular families of intermediate polarity ranging from polyphenols to triglycerides. The presence of flavonoids with anti-oxidant properties and of triterpenoids with potential anti-inflammatory activity was evidenced and cell-wall constituents such as fatty acids, glycolipids, sphingolipids and acylated sterol glycosides were characterised. Some chlorophyll derivatives were also detected. The method developed is appropriate for fast phytochemical evaluation of rose ethyl acetate extract. It produced accurate mass and MS/MS spectra, which permitted identification of a wide range of compounds of intermediate polarity. Copyright © 2015 John Wiley & Sons, Ltd.

  9. EXAFS analysis of cations distribution in structure of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} nanoparticles obtained by hydrothermal method in aloe vera extract solution

    Energy Technology Data Exchange (ETDEWEB)

    Wongpratat, Unchista [Materials Science and Nanotechnology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Maensiri, Santi [School of Physics, Institute of Science, Suranaree University, Nakhonratchasima 30000 (Thailand); Swatsitang, Ekaphan, E-mail: ekaphan@kku.ac.th [Materials Science and Nanotechnology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Integrated Nanotechnology Research Center, Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand (Thailand)

    2016-09-01

    Graphical abstract: It is obvious from the M–H curves at room temperature of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles that partially substitution of the lower Bohr magneton (2 μ{sub B}) and smaller atomic radii (0.55 Å at A site, 0.69 Å at B site) of Ni{sup 2+} ions on the higher Bohr magneton (3 μ{sub B}) and larger atomic radii (0.58 Å at A site, 0.74 Å at B site) of Co{sup 2+} ions can increase the saturation magnetization (M{sub s}) of sample with x = 0.75 to approximately 1.4 times of sample with x = 0, due to the increase of the aspect ratio (surface to volume) of nanoparticles, as a result of particle size decreasing from 37.03 to 12.63 nm. In addition to this, the ferrimagnetic behavior of CoFe{sub 2}O{sub 4} has been changed to superparamagnetic behavior with the dramatic decrease of the coercivity from 1365.60 to 63.15 Oe. - Highlights: • Magnetic behavior of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} NPs depends on Ni content and size of NPs. • Distribution of Co{sup 2+} and Ni{sup 2+} ions in the structure results in the increase of M{sub s}. • Superparamagnetic behavior is observed with increasing of the aspect ratio. • M{sub s} is increased by a factor 1.4 to a value of 57.57 emu/g in Co{sub 0.25}Ni{sub 0.75}Fe{sub 2}O{sub 4}. • H{sub c} is decreased by a factor 20 to a value of 63.15 Oe in Co{sub 0.25}Ni{sub 0.75}Fe{sub 2}O{sub 4}. - Abstract: Effect of cations distribution upon EXAFS analysis on magnetic properties of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles prepared by the hydrothermal method in aloe vera extract solution were studied. XRD analysis confirmed a pure phase of cubic spinel ferrite of all samples. Changes in lattice parameter and particle size depended on the Ni content with partial substitution and site distributions of Co{sup 2+}, Ni{sup 2+} ions of different ionic radii at both tetrahedral and octahedral sites in the

  10. TECHNOLOGICAL ASPECTS CONCERNING PRODUCTION ON ASEXUAL TRACK OF ORNAMENTAL CULTURE OF ALOE ARBORESCENS

    Directory of Open Access Journals (Sweden)

    Mădălina Doltu

    2012-12-01

    Full Text Available Research conducted in culture of Aloe arborescens Mill., in conditions of protected area (greenhouse, had to watch some of technological aspects to obtain some in pot ornamentals plants, competitive plants for european market. Experience has included plants of Aloe arborescens Mill., aged 1-5 years, obtained by vegetative multiplying, asexual. Production technology was applied to aloe plants (multiplying, transplanting, maintenance, taking into account the ecological requirements of specie and possibilities of using automated artificial factors influencing microclimate (shading, ventilation, cooling of production space. Researches has established technological aspects concerning production of plants, to satisfy requirements of specie, for obtained some specimens by superior decorative quality. The results of experienced technology indicate quality of morphological characters, growth increases important of decorative elements (height, leaves, shoots, appearance of flowering at plants aged two years. Culture of Aloe arborescens Mill. in pot, can be practiced all year, just in conditions of protected areas. This culture, realized in the purpose for obtain decorative plants, is a valuable activity of horticulture.

  11. Antinociceptive activity of flower buds extract of Sophora japonica and its main active ingredient quercetin in bee venom-induced rat model

    Directory of Open Access Journals (Sweden)

    Bianling Zhang

    2014-02-01

    Results: The administration of ME from S. japonica and quercetin significantly increased the tolerance to pain in SD rats in comparison to control. The ME of S. japonica and quercetin produced significant inhibition of both the early (neurogenic pain or acute pain, 0-10 min and the late (inflammatory pain or tonic pain, 11-40 min phases of bee venom-induced pain. Furthermore, the ME (100-500 mg/kg, i.p. caused a significant increase in the latency to response in the hot-plate test. However, the quercetin (5-50 mg/kg, i.p. had no significant effect in the hot-plate test. Conclusion: These results suggest that the ME of S. japonica and its main active ingredient quercetin appear to contribute for the antinociceptive property in the bee venom-induced rats. The investigation gives an evidence of potential uses of the flower buds extract of S. japonica and quercetin as medicines of adjunctive therapy pain. [J Intercult Ethnopharmacol 2014; 3(1.000: 9-14

  12. Flavonoid-Rich Extract of Paulownia fortunei Flowers Attenuates Diet-Induced Hyperlipidemia, Hepatic Steatosis and Insulin Resistance in Obesity Mice by AMPK Pathway.

    Science.gov (United States)

    Liu, Chanmin; Ma, Jieqiong; Sun, Jianmei; Cheng, Chao; Feng, Zhaojun; Jiang, Hong; Yang, Wei

    2017-08-30

    The flavonoid-rich extract from Paulownia fortunei flowers (EPF) has been reported to prevent obesity and other lipid metabolism disease. However, the mechanism of its protective effects is not yet clear. The objective of this study was to investigate molecular factors involved in the hypoglycemic and hypolipidemic effects of EPF in obese mice fed a high-fat diet (HFD). Male h ICR (Institute of Cancer Research) mice were fed a HFD containing or not containing the EPF (50 or 100 mg/kg) for eight weeks. EPF reduced body weight gain, lipid accumulation in livers and levels of lipid, glucose and insulin in plasma as well as reduced insulin resistance as compared with the HFD group. EPF significantly decreased serum aminotransferase activity of the HFD group. We observed that EPF administration significantly increased the level of AMP-activated kinase (AMPK) phosphorylation and prevented fat deposits in livers and HepG2 cells, but these effects were blocked by compound C (an AMPK inhibitor). The protective effects of EPF were probably associated with the decrease in HMGCR, SREBP-1c and FAS expressions and the increase in CPT1 and phosphor-IRS-1 expressions. Our results suggest that EPF might be a potential natural candidate for the treatment and/or prevention of overweight and hepatic and metabolic-related alterations induced by HFD.

  13. Flavonoid-Rich Extract of Paulownia fortunei Flowers Attenuates Diet-Induced Hyperlipidemia, Hepatic Steatosis and Insulin Resistance in Obesity Mice by AMPK Pathway

    Directory of Open Access Journals (Sweden)

    Chanmin Liu

    2017-08-01

    Full Text Available The flavonoid-rich extract from Paulownia fortunei flowers (EPF has been reported to prevent obesity and other lipid metabolism disease. However, the mechanism of its protective effects is not yet clear. The objective of this study was to investigate molecular factors involved in the hypoglycemic and hypolipidemic effects of EPF in obese mice fed a high-fat diet (HFD. Male h ICR (Institute of Cancer Research mice were fed a HFD containing or not containing the EPF (50 or 100 mg/kg for eight weeks. EPF reduced body weight gain, lipid accumulation in livers and levels of lipid, glucose and insulin in plasma as well as reduced insulin resistance as compared with the HFD group. EPF significantly decreased serum aminotransferase activity of the HFD group. We observed that EPF administration significantly increased the level of AMP-activated kinase (AMPK phosphorylation and prevented fat deposits in livers and HepG2 cells, but these effects were blocked by compound C (an AMPK inhibitor. The protective effects of EPF were probably associated with the decrease in HMGCR, SREBP-1c and FAS expressions and the increase in CPT1 and phosphor-IRS-1 expressions. Our results suggest that EPF might be a potential natural candidate for the treatment and/or prevention of overweight and hepatic and metabolic-related alterations induced by HFD.

  14. Effect of meadowsweet flower extract-pullulan coatings on rhizopus rot development and postharvest quality of cold-stored red peppers.

    Science.gov (United States)

    Synowiec, Alicja; Gniewosz, Małgorzata; Kraśniewska, Karolina; Chlebowska-Śmigiel, Anna; Przybył, Jarosław L; Bączek, Katarzyna; Węglarz, Zenon

    2014-08-25

    The study involved an examination of the antifungal activity on red peppers of pullulan coating (P) and pullulan coating containing either water-ethanol (P + eEMF) or ethanol extract of meadowsweet flowers (P + eEMF). Pullulan was obtained from a culture of Aureobasidium pullulans B-1 mutant. Both non-inoculated peppers and those artificially inoculated with Rhizopus arrhizus were coated and incubated at 24 °C for 5 days. The intensity of the decay caused by Rhizopus arrhizus in the peppers with P and P + eEMF coatings was nearly 3-fold lower, and in the case of P + weEMF 5-fold lower, than that observed in the control peppers. Additionally, the P + weEMF coating decreased, almost two-fold the severity of pepper decay compared to other samples. The influence of coating of pepper postharvest quality was examined after 30 days of storage at 6 °C and 70%-75% RH. All coatings formed a thin and well-attached additional layer of an intensified gloss. During storage, color, total soluble solid content and weight loss of coated peppers were subject to lower changes in comparison with uncoated ones. The results indicate the possibility of the application of pullulan coatings containing MFEs as an alternative to the chemical fungicides used to combat pepper postharvest diseases.

  15. Effect of Meadowsweet Flower Extract-Pullulan Coatings on Rhizopus Rot Development and Postharvest Quality of Cold-Stored Red Peppers

    Directory of Open Access Journals (Sweden)

    Alicja Synowiec

    2014-08-01

    Full Text Available The study involved an examination of the antifungal activity on red peppers of pullulan coating (P and pullulan coating containing either water-ethanol (P + eEMF or ethanol extract of meadowsweet flowers (P + eEMF. Pullulan was obtained from a culture of Aureobasidium pullulans B-1 mutant. Both non-inoculated peppers and those artificially inoculated with Rhizopus arrhizus were coated and incubated at 24 °C for 5 days. The intensity of the decay caused by Rhizopus arrhizus in the peppers with P and P + eEMF coatings was nearly 3-fold lower, and in the case of P + weEMF 5-fold lower, than that observed in the control peppers. Additionally, the P + weEMF coating decreased, almost two-fold the severity of pepper decay compared to other samples. The influence of coating of pepper postharvest quality was examined after 30 days of storage at 6 °C and 70%–75% RH. All coatings formed a thin and well-attached additional layer of an intensified gloss. During storage, color, total soluble solid content and weight loss of coated peppers were subject to lower changes in comparison with uncoated ones. The results indicate the possibility of the application of pullulan coatings containing MFEs as an alternative to the chemical fungicides used to combat pepper postharvest diseases.

  16. Daya Hambat Ekstrak Aloe Vera terhadap pertumbuhan Staphylococcus Aureus

    OpenAIRE

    Rahmat, drg.Sp,Pros

    2011-01-01

    Dari hasil penelitian , maka dapat disimpulkan bahwa ekstrak Aloe Vera dapat menghambat pertumbuhan bakteri Stafhylococcus aureus, dan kadar hambat minimal ekstrak Aloe Vera adalah pada konsentrasi 25%. Tujuan Penelitan Ini adalah untuk mengetahui efektifitas ekstrak Aloe vera dalam menghambat pertumbuhan bakteri Stafhylococcus aureus dan daya hambat menimal, (DHM) terhadap pertumbuhan bakteri tersebut. Metode yang digunakan adalah pertumbuhan ekstrak Aloe vera, penegnceran ekstrak , pemur...

  17. Silver Nanoparticles Biosynthesized Using Achillea biebersteinii Flower Extract: Apoptosis Induction in MCF-7 Cells via Caspase Activation and Regulation of Bax and Bcl-2 Gene Expression

    Directory of Open Access Journals (Sweden)

    Javad Baharara

    2015-02-01

    Full Text Available Silver nanoparticles (Ag-NPs, the most popular nanoparticles, possess unique properties. Achillea biebersteinii is a plant of the Asteraceae family rich in active antitumor components. The aim of this research was the characterization and investigation of the cytotoxic properties of Ag-NPs synthesized using A. biebersteinii flower extract, on a human breast cancer cell line. The Ag-NPs were synthesized after approximately 180 min of reaction at 40 °C, then they were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR, transmission electron microscopy (TEM and dynamic light scattering (DLS. The anti-apoptosis effect of Ag-NPs on the MCF-7 cell line was investigated by MTT assay, DAPI and acridine orange staining and caspase activity. The transcriptional expression of bax, bcl-2, caspase-3, -8 and -9 were also evaluated by RT-PCR. The TEM images revealed that the Ag-NPs morphology had a different shape. The DLS indicated that the average hydrodynamic diameter of the biosynthesized Ag-NPs was around 12 nm. By UV-visible spectroscopy the strongest absorbance peak was observed at 460 nm. The FTIR results also showed interaction between the plant extract and Ag-NPs due to the similarity in the peak patterns. The EDS results showed that Ag-NPs display an absorption peak at 3 keV, indicating the presence of the element silver. The Ag-NPs caused a dose-dependent decrease in cell viability, fragmentation in nucleic acid, inhibited the proliferation and induction of apoptosis on MCF-7 by suppressing specific cell cycle genes, and simulation programmed cell dead genes. Further investigation is required to establish the potential of this novel and promising approach in cancer therapy.

  18. Effects of Six Weeks Endurance Training and Aloe Vera Supplementation on COX-2 and VEGF Levels in Mice with Breast Cancer

    Science.gov (United States)

    Shirali, Saeed; Barari, Alireza; Hosseini, Seyed Ahmad; Khodadi, Elaheh

    2017-01-01

    The aim of this study was to determine effects of six weeks endurance training and Aloe Vera supplementation on COX-2 and VEGF levels in mice with breast cancer. For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Aloe Vera and Aloe Vera + training. Breast cancer tumors were generated in mice by implantind. The training program comprised six weeks of swimming training accomplished in three sessions per week. Training time started with 10 minutes on the first day and increased to 60 minutes in the second week and the water flow rate was increased from 7 to 15 liters per minute at a constant rate. Aloe Vera extract at a dose of 300 mg/kg BW was administrated to rats by intraperitoneal injection. At the end of the study period, rats were anesthetized and blood samples were taken. Significant differences were concluded at pAloe Vera extract caused significant decrease in the COX-2 level in the cancer group. Also, in the training (swimming exercise) and Aloe Vera + training cancer groups, we observed significant decrease in the VEGF level as compared to controls. Our results suggest that Aloe Vera and training inhibit the COX pathway and cause decrease production of prostaglandin E2. Hence administration of Aloe Vera in combination with endurance training might synergistically improve the host milieu in mice bearing breast cancers. Creative Commons Attribution License

  19. Extraction of Antioxidant Components from Bidens pilosa Flowers and Their Uptake by Human Intestinal Caco-2 Cells

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    Charng-Cherng Chyau

    2013-01-01

    Full Text Available Bidens pilosa L. var. radiata (BPR, Asteraceae is a commonly used folk medicine for treating various disorders such as diabetes, inflammation and hypertension. Recent studies to determine its chemical composition have revealed three di-O-caffeoylquinic acids (DiCQAs and three polyacetylene glucosides (PGAs to be among the major bioactive markers. To obtain the major compounds of these two chemical classes, the ethyl acetate fraction (EM obtained using liquid-liquid partition from the methanol extract resulted in a fraction with the highest total phenolic and total flavonoid contents and antioxidant activities in radical scavenging and ferric reducing power assays. To assess the bioavailability of EM, we examined the in vitro uptake using the Caco-2 human colonic cell line. The apparent permeability coefficient (Papp for each of the compounds within PGAs measured in both apical (AP to basolateral (BL and BL to AP was found to preferentially appear BL to AP direction, indicated that a basolateral to apical efflux system was detected in the study. DiCQAs had a lower efflux ratio than those from PGAs (2.32–3.67 vs. 6.03–78.36. Thus, it strongly implies that most of the DiCQAs are better absorbed than the PGAs.

  20. Antioxidative and Anticanceric Activities of Magnolia (Magnolia denudata Flower Petal Extract Fermented by Pediococcus acidilactici KCCM 11614

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    Hye Park

    2015-07-01

    Full Text Available In this study, the effects of magnolia (Magnolia (M. denudata extract fermentation in increasing the extract’s antioxidative and anticancer activities were investigated. Magnolia was fermented by Pediococcus acidilactici KCCM 11614. The total phenolic content was determined by the Folin-Ciocalteu’s method and the antioxidative effects by 1,1-diphenyl-2-picrylhydrazy (DPPH and ferric reducing ability of plasma (FRAP assay. Anticancer activity against cancer and normal cells was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT. Total phenolic content during fermentation increased from 38.1 to 47.0 mg gallic acid equivalent (GAE/g of solid matter. The radical scavenging activity was 91.4% after 72 h fermentation. Fermented magnolia’s antioxidative effect was threefold higher than that of the (non-fermented control. Fermentation (48 h increased anticanceric activity against AGS, LoVo, and MCF-7 cancer cells 1.29- to 1.36-fold compared with that of the control, but did not affect MRC-5 (normal cells, suggesting that fermented magnolia could be used as a natural antioxidative and anticancer agent.

  1. Flowers in Their Variety.

    Science.gov (United States)

    Flannery, Maura C.

    2002-01-01

    Describes the diversity of flowers with regard to the flower paintings of Pierre-Joseph Redoute, books about flowers, and research in genetic studies. Discusses gardening flowers and flowering strategies and criticizes the fact that biology education has moved steadily away from plants. (KHR)

  2. Flower pigment analysis of Melastoma malabathricum | Janna ...

    African Journals Online (AJOL)

    The objective of this study is to analyse the colour pigment, anthocyanin, that can be detected in flower and their stability in extracted form. All the analysed results will be used in the next study for the production of new food colouring material. From the observation, it shows that S3 flower developmental stage contains the ...

  3. Evaluation of the anti-ulcer and toxicity profile of Aloe buettneri in ...

    African Journals Online (AJOL)

    The anti-ulcerogenic potential of the leaf methanol extract of Aloe buettneri A. Berger was investigated using three methods of gastric lesion induction in experimental Wistar rats (150-200 g) and mice (20-25 g): 1. HCl/ethanol-induced gastic lesions, 2. Indomethacin-HCl/ethanol-induced gastric lesions, and 3, Pylorus ...

  4. Chromatographic separation and detection methods of Aloe arborescens Miller constituents: A systematic review.

    Science.gov (United States)

    Nazeam, Jilan A; Gad, Haidy A; El-Hefnawy, Hala M; Singab, Abdel-Naser B

    2017-07-15

    Aloe arborescens Miller (Family Asphodelaceae) is a member of genus Aloe, which is used in traditional medicine to cure various diseases. The extracts of the plant have been reported to possess anticancer, immunomodulator, antidiabetic, anti-inflammatory and antioxidant activities. The phytochemical investigations have revealed diverse chemical constituents, including phenolics [anthraquinones, anthrones, pyrones, chromones and coumarins], polysaccharides [arborans [(1-4) linked glucomannans, polysaccharide (A, B and C): (A: a linear (1-6)-O-α-glucan, B: a branching (1-2)-O-l-arabinose with (1-2)-O-d-galactose linkages and C: (1-4)-O-β-mannan with 18% acetyl group)

  5. Green synthesis and characterization of silver nanoparticle using Aloe barbadensis

    Energy Technology Data Exchange (ETDEWEB)

    Thappily, Praveen, E-mail: pravvmon@gmail.com, E-mail: shiiuvenus@gmail.com; Shiju, K., E-mail: pravvmon@gmail.com, E-mail: shiiuvenus@gmail.com [Laboratory for Molecular Photonics and Electronics (LAMP), Department of Physics, National Institute of Technology, Calicut, Kerala 673601 (India)

    2014-10-15

    Green synthesis of silver nanoparticles was achieved by simple visible light irradiation using aloe barbadensis leaf extract as reducing agent. UV-Vis spectroscopic analysis was used for confirmation of the successful formation of nanoparticles. Investigated the effect of light irradiation time on the light absorption of the nanoparticles. It is observed that upto 25 minutes of light irradiation, the absorption is linearly increasing with time and after that it becomes saturated. Finally, theoretically fitted the time-absorption graph and modeled a relation between them with the help of simulation software.

  6. Green synthesis and characterization of silver nanoparticle using Aloe barbadensis

    Science.gov (United States)

    Thappily, Praveen; Shiju, K.

    2014-10-01

    Green synthesis of silver nanoparticles was achieved by simple visible light irradiation using aloe barbadensis leaf extract as reducing agent. UV-Vis spectroscopic analysis was used for confirmation of the successful formation of nanoparticles. Investigated the effect of light irradiation time on the light absorption of the nanoparticles. It is observed that upto 25 minutes of light irradiation, the absorption is linearly increasing with time and after that it becomes saturated. Finally, theoretically fitted the time-absorption graph and modeled a relation between them with the help of simulation software.

  7. Controlled release of an extract of Calendula officinalis flowers from a system based on the incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro performance.

    Science.gov (United States)

    Jiménez, Ronald A; Millán, Diana; Suesca, Edward; Sosnik, Alejandro; Fontanilla, Marta R

    2015-06-01

    Aiming to develop biological skin dresses with improved performance in the treatment of skin wounds, acellular collagen I scaffolds were modified with polymeric microparticles and the subsequent loading of a hydroglycolic extract of Calendula officinalis flowers. Microparticles made of gelatin-collagen were produced by a water-in-oil emulsion/cross-linking method. Thereafter, these microparticles were mixed with collagen suspensions at three increasing concentrations and the resulting mixtures lyophilized to make microparticle-loaded porous collagen scaffolds. Resistance to enzymatic degradation, ability to associate with the C. officinalis extract, and the extract release profile of the three gelatin-collagen microparticle-scaffold prototypes were assessed in vitro and compared to collagen scaffolds without microparticles used as control. Data indicated that the incorporation of gelatin-collagen microparticles increased the resistance of the scaffolds to in vitro enzymatic degradation, as well as their association with the C. officinalis flower extract. In addition, a sharp decrease in cytotoxicity, as well as more prolonged release of the extract, was attained. Overall results support the potential of these systems to develop innovative dermal substitutes with improved features. Furthermore, the gelatin-collagen mixture represents a low-cost and scalable alternative with high clinical transferability, especially appealing in developing countries.

  8. In vitro antibacterial activity of the crude methanol extract of Woodfordia fruticosa Kurz. flower (Lythraceae Atividade antibacteriana in vitro do extrato metanólico bruto da flor Woodfordia fruticosa Kurz. (Lythraceae

    Directory of Open Access Journals (Sweden)

    Jigna Parekh

    2007-06-01

    Full Text Available The antibacterial activity of the crude methanol extract of Woodfordia fruticosa Kurz. flower was evaluated at two different concentrations by the agar well diffusion method. The methanol extract of the flower exhibited antibacterial activity at varied levels except against Bacillus subtilis and Micrococcus flavus. The methanol extract was most active against Pseudomonas pseudoalcaligenes. The extract was more active against Gram-negative bacteria as compared to Gram-positive. The inhibitory effect of the extract was compared with standard antibiotics, amoxicillin and ciprofloxacin.A atividade antibacteriana do extrato metanólico bruto da flor Woodfordia fruticosa Kurz. (Lythraceae foi avaliada em duas concentrações diferentes através do método de difusão em poços. A atividade antibacteriana ocorreu em diferentes níveis, exceto contra Bacillus subtilis e Micrococcus flavus. O extrato metanólico foi mais ativo contra Pseudomonas pseudoalcaligens. O extrato foi mais ativo contra bactérias Gram negativas do que bactérias Gram positivas. O efeito inibitório do extrato foi comparado ao dos antibióticos padrão amoxicilina e ciprofloxacina.

  9. Extract of Woodfordia fruticosa flowers ameliorates hyperglycemia, oxidative stress and improves β-cell function in streptozotocin-nicotinamide induced diabetic rats.

    Science.gov (United States)

    Arya, Aditya; Al-Obaidi, Mazen M Jamil; Karim, Rustini Binti; Taha, Hairin; Khan, Ataul Karim; Shahid, Nayiar; Sayem, Abu Sadat; Looi, Chung Yeng; Mustafa, Mohd Rais; Mohd, Mustafa Ali; Ali, Hapipah Mohd

    2015-12-04

    The art of Ayurveda and the traditional healing system in India have reflected the ethnomedicinal importance of the plant Woodfordia fruticosa Kurtz, which demonstrates its vast usage in the Ayurvedic preparations as well as in the management of diabetes by the traditional healers. The study aimed to ascertain the antidiabetic potential of W. fruticosa flower methanolic extract (WF) on Streptozotocin (STZ)-nicotinamide-induced diabetic rat model. Diabetes was induced in Sprague Dawley (SD) rats by STZ-nicotinamide and thereafter diabetic rats were treated with three different doses of WF (100, 200 and 400mg/kg body weight) respectively and glibenclamide as a positive control. Biochemical parameters such as blood glucose, serum insulin and C-peptide levels were measured with oxidative stress markers. Furthermore, histology of liver and pancreas was carried out to evaluate glycogen content and β-cell structures. Moreover, immunohistochemistry and western blot analysis were performed on kidney and pancreas tissues to determine renal Bcl-2, pancreatic insulin and glucose transporter (GLUT-2, 4) protein expression in all the experimental groups. The acute toxicity study showed non-toxic nature of all the three doses of WF. Further, studies on diabetic rats exhibited anti-hyperglycemic effects by upregulating serum insulin and C-peptide levels. Similarly, WF shown to ameliorate oxidative stress by downregulating LPO levels and augmenting the antioxidant enzyme (ABTS). Furthermore, histopathological analysis demonstrate recovery in the structural degeneration of β-cells mass of pancreas tissue with increase in the liver glycogen content of the diabetic rats. Interestingly, protective nature of the extract was further revealed by the immunohistochemical study result which displayed upregulation in the insulin and renal Bcl-2 expression, the anti apoptosis protein. Moreover, western blot result have shown slight alteration in the GLUT-2 and GLUT-4 protein expression with

  10. Physicochemical characterization of microwave assisted synthesis of silver nanoparticles using Aloe Vera (Aloe barbadensis)

    Science.gov (United States)

    Kuponiyi, Abiola John

    Biosynthesis of silver nanoparticles (AgNP) using different biological extracts is gaining recognition for its numerous applications in different disciplines. Although different approaches (physical and chemical) have been used for the synthesis of AgNP, the green chemistry method is most preferable because of its high efficacy, cost effectiveness, and environmental benignity. Aloe Vera (AV) contains chemical compounds (anthraquinones) that are known to possess antibacterial, antivirus and anticancer properties and the extract is a good chemical reduction agent for AgNP. Hence, it was hypothesized that a microwave assisted synthesis will produce highly concentrated, homogeneous, stable and biologically active AgNP. Thus, the main objective of the study was to evaluate the effect of microwave assisted synthesis of AgNP, the effect of pulse laser treatment on size reduction of a microwave synthesized AgNP, and the physicochemical characterization of AgNP synthesized with Aloe Vera water and ethanol extract. The experiment was conducted in two phases. Phase 1 was first conducted to optimize the experimental variables, thus establishing the optimum variables to apply in the second phase. The experiment in Phase 1 was conducted using three-factor factorial experimental design comprised of the following factors: 1) Extraction Solvent, 2) Heating Methods, 3) pH; and their corresponding levels were water and ethanol, conventional and microwave, pH (7, 8, 10 and 12), respectively. All synthesis was conducted at constant temperature of 80°C. Phase II experimental treatments were Laser ablation (0, 5, and 10 min) and Storage time (Week 1, 2 & 3). The Phase I of the results showed that increased AgNP concentrations were significantly (p 0.05) impact the particle size distribution. Hence, the Zeta potential of the particles has values typically ranging between +100 mV to -100 mV, hence indicative of colloidal stability matrix. Furthermore, the Polydispersity indexes of Week 1

  11. Orbital flower

    Science.gov (United States)

    Szucs-Csillik, Iharka

    2017-11-01

    The regularizing techniques known as Kustaanheimo-Stiefel (KS) transformation have investigated. It has proved that it is very useful in n-body simulations, where it helps to handle close encounters. This paper shows how the basic transformation is a starting point for a family of polynomial coupled function. This interpretation becomes simply on writing KS transformations in quaternion form, which also helps to derive concise expressions for regularized equations of motion. Even if the KS regularization method is more easy to use, it is interesting to encapsulate the KS transformation in a family of methods, which all conserve the KS transformations' properties. Further, an interesting point of view is considering, the orbital shapes of the restricted three-body problem (also regularized restricted three-body problem) for different initial conditions has compared with flower pattern.

  12. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

    Science.gov (United States)

    Winters, Wallace D.; Scott, Michael; David, Andrew; Gillis, Glenn; Stoufflet, Thaya; Nair, Anand; Kousoulas, Konstantine

    2013-01-01

    Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL) aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks. PMID:23554812

  13. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

    Directory of Open Access Journals (Sweden)

    Inder Sehgal

    2013-01-01

    Full Text Available Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

  14. Anticholinesterase activity and chemical profile of an active chromatographic fraction of ethanolic extract from Bellis perennis L. (Asteraceae) flowers; Atividade anticolinesterasica e perfil quimico de uma fracao cromatografica ativa do extrato etanolico das flores Bellis perennis L. (Asteraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Thiago Henrique Costa; Santos, Pauline Sousa dos; Freitas, Rivelilson Mendes de, E-mail: rivelilson@pq.cnpq.br [Universidade Federal do Piaui (UFPI), Teresina, PI (Brazil). Centro de Ciencias da Saude. Departamento de Bioquimica e Farmacologia; Carvalho, Rusbene Bruno Fonseca de; Melo, Cassio Herbert Santos de [Universidade Federal do Piaui (UFPI), Teresina, PI (Brazil). Centro de Ciencias da Natureza. Departamento de Quimica; David, Juceni Pereira [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Faculdade de Farmacia; David, Jorge Mauricio; Lima, Luciano Silva [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Instituto de Quimica

    2013-09-01

    This work describes the isolation of an active flavonoid fraction and identification of isorhamnetin 3-O-{beta}-D-(6''-acetyl)- alactopyranoside from flowers of B. perennis, and also the evaluation of anticholinesterase (AChE) activity of ethanolic extract from flowers (EEF) and the active fraction. The chemical structure of the flavonoid was defined on the basis of spectroscopic {sup 1}H NMR, IR and UV data. EEF or flavonoid reduces AChE activity in vivo, while flavonoid also reduces AChE activity in vitro, showing a value of 1.49 {mu}M for 50% inhibitory concentration (IC{sub 50}), suggesting potential use as an insecticide or in the treatment of neurodegenerative diseases such as Alzheimer's disease. (author)

  15. Physicochemical characterization of silver nanoparticles synthesize using Aloe Vera (Aloe barbadensis)

    Science.gov (United States)

    Kuponiyi, Abiola; Kassama, Lamin; Kukhtareva, Tatiana

    2014-08-01

    Production of silver nanoparticles (AgNPs) using different biological methods is gaining recognition due to their multiple applications. Although, several physical and chemical methods have been used for the synthesis and stabilizing of AgNPs, yet, a green chemistry method is preferable because it is cost effective and environmentally friendly. The synthesis was done using Aloe Vera (AV) extract because it has chemical compounds such as "Antrokinon" that are known for its antibacterial, antivirus and anticancer properties. We hypothesize that AV extract can produce a stable nanoparticles within the 100 nm range and be biologically active. The biological compounds were extracted from AV skin with water and ethanol which was used as the reduction agent for the synthesis of nanoparticles. The biological extract and AgNO3 were blended and heated to synthesize AgNPs. The reaction process was monitored using UV-Visible spectroscopy. Fourier Transfer Infrared spectroscopy (FTIR) was used for the characterization of biological compounds and their substituent groups before and after the reaction process. Dynamic Light scattering (DLS) method was used to characterize particle size of AgNPs and their biomolecular stability. Results showed that biological compounds such as aliphatic amines, alkenes (=C-H), alkanes (C-H), alcohol (O-H) and unsaturated esters(C-O), which has an average particle size of 109 and 215.8 nm and polydispersity index of 0.451 and 0.375 for ethanol and water extract, respectively. According to TEM measurements the size of AgNPs are in the range 5-20 nm The results suggested that ethanol derived AgNPs contained higher yield of organic compounds, thus has better solubility power than water. Ag NPs can be used to control salmonella in poultry industry.

  16. Acute toxicity and laxative activity of Aloe ferox resin

    Directory of Open Access Journals (Sweden)

    Vanessa R. L. Celestino

    2013-04-01

    Full Text Available Aloe ferox Mill., Xanthorrhoeaceae, resin is the solid residue obtained by evaporating the latex that drains from the leaves transversally cut. Aloe ferox has been used in folk medicine as anti-inflammatory, immunostimulant, anti-bacterial, anti-fungal, antitumor, laxative and to heal wounds and burns. The effects of the oral administration of A. ferox resin (10, 25, 50, 100 and 200 mg/kg were evaluated on intestinal transit in mice and its acute toxicity (5.0 g/kg in Wistar rats. The hydroxyanthracene derivatives present in the resin were expressed as aloin, identified by thin layer chromatography and quantified by spectrophotometry. The aloin (Rf 0.35 was identified and the percentage of hydroxyanthracene derivates expressed as aloin was 33.5%. A. ferox resin extract (50, 100 and 200 mg/kg increased the gastrointestinal motility at a 30 min interval at 93.5, 91.8 and 93.8%, respectively, when compared to control group (46.5%. A single oral dose of the A. ferox resin extract did not induce signs of toxicity or death. Thus, the results demonstrate that A. ferox has laxative activity and that it is nontoxic, since LD50 could not be estimated and it is possibly higher than 5.0 g/kg.

  17. Aloe vera Induced Biomimetic Assemblage of Nucleobase into Nanosized Particles

    Science.gov (United States)

    Chauhan, Arun; Zubair, Swaleha; Sherwani, Asif; Owais, Mohammad

    2012-01-01

    Aim Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, biomimetic (employing microorganism/plants) synthesis of metal and inorganic materials nano-particles has emerged as a simple and viable strategy. In the present study, we have extended biological synthesis of nano-particles to organic molecules, namely the anticancer agent 5-fluorouracil (5-FU), using Aloe vera leaf extract. Methodology The 5-FU nano- particles synthesized by using Aloe vera leaf extract were characterized by UV, FT-IR and fluorescence spectroscopic techniques. The size and shape of the synthesized nanoparticles were determined by TEM, while crystalline nature of 5-FU particles was established by X-ray diffraction study. The cytotoxic effects of 5-FU nanoparticles were assessed against HT-29 and Caco-2 (human adenocarcinoma colorectal) cell lines. Results Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines. Conclusion In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints. PMID:22403622

  18. Acute toxicity and laxative activity of Aloe ferox resin

    Directory of Open Access Journals (Sweden)

    Vanessa R. L. Celestino

    2013-02-01

    Full Text Available Aloe ferox Mill., Xanthorrhoeaceae, resin is the solid residue obtained by evaporating the latex that drains from the leaves transversally cut. Aloe ferox has been used in folk medicine as anti-inflammatory, immunostimulant, anti-bacterial, anti-fungal, antitumor, laxative and to heal wounds and burns. The effects of the oral administration of A. ferox resin (10, 25, 50, 100 and 200 mg/kg were evaluated on intestinal transit in mice and its acute toxicity (5.0 g/kg in Wistar rats. The hydroxyanthracene derivatives present in the resin were expressed as aloin, identified by thin layer chromatography and quantified by spectrophotometry. The aloin (Rf 0.35 was identified and the percentage of hydroxyanthracene derivates expressed as aloin was 33.5%. A. ferox resin extract (50, 100 and 200 mg/kg increased the gastrointestinal motility at a 30 min interval at 93.5, 91.8 and 93.8%, respectively, when compared to control group (46.5%. A single oral dose of the A. ferox resin extract did not induce signs of toxicity or death. Thus, the results demonstrate that A. ferox has laxative activity and that it is nontoxic, since LD50 could not be estimated and it is possibly higher than 5.0 g/kg.

  19. Aloe vera induced biomimetic assemblage of nucleobase into nanosized particles.

    Science.gov (United States)

    Chauhan, Arun; Zubair, Swaleha; Sherwani, Asif; Owais, Mohammad

    2012-01-01

    Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, biomimetic (employing microorganism/plants) synthesis of metal and inorganic materials nano-particles has emerged as a simple and viable strategy. In the present study, we have extended biological synthesis of nano-particles to organic molecules, namely the anticancer agent 5-fluorouracil (5-FU), using Aloe vera leaf extract. The 5-FU nano- particles synthesized by using Aloe vera leaf extract were characterized by UV, FT-IR and fluorescence spectroscopic techniques. The size and shape of the synthesized nanoparticles were determined by TEM, while crystalline nature of 5-FU particles was established by X-ray diffraction study. The cytotoxic effects of 5-FU nanoparticles were assessed against HT-29 and Caco-2 (human adenocarcinoma colorectal) cell lines. Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines. In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints.

  20. Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens.

    Science.gov (United States)

    Akhtar, Masood; Hai, Abdul; Awais, Mian Muhammad; Iqbal, Zafar; Muhammad, Faqir; ul Haq, Ahsan; Anwar, Muhammad Irfan

    2012-05-25

    This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (pcoccidiosis in industrial broiler chickens. Copyright © 2011 Elsevier B.V. All rights reserved.

  1. The effect of Aloe vera gel on viability of dental pulp stem cells.

    Science.gov (United States)

    Sholehvar, Fatemeh; Mehrabani, Davood; Yaghmaei, Parichehr; Vahdati, Akbar

    2016-10-01

    Dental pulp stem cells (DPSCs) can play a prominent role in tissue regeneration. Aloe vera L. (Liliaceae) contains the polysaccharide of acemannan that was shown to be a trigger factor for cell proliferation, differentiation, mineralization, and dentin formation. This study sought to determine the viability of DPSCs in Aloe vera in comparison with Hank's balanced salt solution (HBSS). Twelve rabbits underwent anesthesia, and their incisor teeth were extracted; the pulp tissue was removed, chopped, treated with collagenase and plated in culture flasks. DPSCs from passage 3 were cultured in 24-well plates, and after 3 days, the culture media changed to 10, 25, 50, and 100% concentrations of Aloe vera at intervals of 45 and 90 min and 3 and 6 h. Distilled water was used as negative and HBSS as positive control for comparison. The cell morphology, viability, population doubling time (PDT), and growth kinetics were evaluated. RT-PCR was carried out for characterization and karyotyping for chromosomal stability. Aloe vera showed a significant higher viability than HBSS (74.74%). The 50% Aloe vera showed higher viability (97.73%) than other concentrations. PDT in 50% concentration was 35.1 h and for HBSS was 49.5 h. DPSCs were spindle shaped and were positive for CD73 and negative for CD34 and CD45. Karyotyping was normal. Aloe vera as an inexpensive and available herb can improve survival of avulsed or broken teeth in emergency cases as a transfer media. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  2. Acceleration of the alcohol oxidation rate in rats with aloin, a quinone derivative of Aloe.

    Science.gov (United States)

    Chung, J H; Cheong, J C; Lee, J Y; Roh, H K; Cha, Y N

    1996-11-08

    Aloe contains abundant aloin, a C-glycoside derivative of anthraquinone. Based on recent reports indicating that the water extract of Aloe enhances the ethanol oxidation rate and also that quinones, in general, have a functional role in elevating the alcohol oxidation rate in vivo, we have attempted to identify the quinone derivative contained in Aloe that could increase the alcohol oxidation rate. Upon oral administration of aloin (300 mg/kg) given 12 hr prior to the administration of alcohol (3.0 g/kg), the blood alcohol area under the curve (AUC) was found to be decreased significantly (by 40%). This was supported by increases in the rates of blood alcohol elimination and the disappearance of alcohol from the body by 45 and 50%, respectively. Analysis of hepatic triglyceride (TG) levels revealed that both the ethanol and the aloin treatment alone significantly increased the TG levels in a comparable manner; however, the level obtained by the combined treatment of aloin and ethanol was not statistically different from that produced by either treatment alone. The levels of serum L-aspartate:2-oxoglutarate aminotransferase (AST) and L-alanine:2-oxoglutarate aminotransferase (ALT) activities were not increased by acute alcohol intoxication, aloin alone, or by the combined treatment of alcohol and aloin. Pretreatments with aloe-emodin, the anthraquinone aglycone of aloin, resulted in a significantly decreased blood alcohol AUC and an increase in the rate of ethanol disappearance. These results suggested that when the aloin localized primarily in the skin of Aloe is ingested, aloe-emodin (the quinone aglycone) may be released, and the released quinone may produce acceleration of the ethanol metabolism rate in vivo.

  3. An HPLC Procedure for the Quantification of Aloin in Latex and Gel from Aloe barbadensis Leaves.

    Science.gov (United States)

    Sánchez-Machado, Dalia I; López-Cervantes, Jaime; Mariscal-Domínguez, María F; Cruz-Flores, Paola; Campas-Baypoli, Olga N; Cantú-Soto, Ernesto U; Sanches-Silva, Ana

    2017-03-01

    Aloin is an anthraquinone-C-glycoside present in Aloe vera. This compound is extremely variable among different species and highly depends on the growing conditions of the plants. The quantification of aloin in different extraction preparations has been a frequent problem due to the high instability of the compound. The aim of the present study is to develop and validated an analytical method for aloin detection in fresh and dry samples of Aloe barbadensis gel and latex using high performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Phosphate buffered saline (pH 3) was selected as the extraction solvent. The aloin was separated using a Zorbax Eclipse AAA column (4.6 × 150 mm) at 35°C, and water and acetonitrile were used as the mobile phase at a flow rate of 0.9 mL/min. The linearity was satisfactory with a correlation coefficient greater than 0.999. Under these conditions, the method precision (relative standard deviation) was 3.71% for FL, 4.41% for dry latex, 0.81% for fresh gel and 4.42% for dry gel samples. Aloe latex was determined to have a greater amount of aloin than aloe gel. The method validation was satisfactory and exhibited adequate linearity, repeatability and accuracy. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  4. Evaluating ALOS AW3D30 data

    Science.gov (United States)

    Nikolakopoulos, Konstantinos G.

    2017-09-01

    A global digital surface model dataset named ALOS Global Digital Surface Model (AW3D30) with a horizontal resolution of approx. 30-meter mesh (1 arcsec) has been released by the Japan Aerospace Exploration Agency (JAXA). The dataset has been compiled with images acquired by the Advanced Land Observing Satellite "DAICHI" (ALOS) and it is published based on the DSM dataset (5-meter mesh version) of the "World 3D Topographic Data", which is the most precise global-scale elevation data at this time, and its elevation precision is also at a world-leading level as a 30-meter mesh version. In this study the accuracy of ALOS AW3D30 was examined. For an area with complex geomorphologic characteristics DSM from ALOS stereo pairs were created with classical photogrammetric techniques. Those DSMs were compared with the ALOS AW3D30. Points of certified elevation collected with DGPS have been used to estimate the accuracy of the DSM. The elevation difference between the two DSMs was calculated. 2D RMSE, correlation and the percentile value were also computed and the results are presented.

  5. Fenología reproductiva y anatomía floral de las plantas Aloe vera y Aloe saponaria (Aloaceae en Cumaná, Venezuela

    Directory of Open Access Journals (Sweden)

    Róger Velásquez-Arenas

    2008-09-01

    Full Text Available Se analizó la fenología reproductiva y aspectos anatómicos florales en dos especies de Aloe conservadas en condiciones de vivero en el oriente de Venezuela, de septiembre 2001 a septiembre 2002. A. vera floreció de diciembre a mayo; A. saponaria de noviembre a agosto. En ambas especies, la antesis duró 48 h, la dehiscencia de la antera coincidió con la apertura del perianto, la receptividad del estigma fue mayor al segundo días de antesis y la producción de néctar alcanzó 0.34 ml/flor. En A. vera se observaron 228±77 flores de color amarillo sobre escapos extensos de 76±11 cm y 1-3 ejes; a diferencia de A. saponaria con 94±33 flores anaranjadas en escapos más compactos de 62±5 cm y 3-5 ejes. La relación polen/óvulo fue de 4 151.2 en A. vera y 3 247.1 en A. saponaria, clasificándose como xenógamas obligatorias. Se registraron visitas frecuentes de Apis mellifera, Trigona sp., Poliste sp., Eumenes sp., Vespa sp., Leucippus fallax y Amazilia tobaci. A pesar de que ambas especies manifestaron una elevada producción de polen, néctar y la presencia de potenciales polinizadores, al término de la floración, sólo se observaron frutos en A. saponaria, con una eficiencia reproductiva de 12%, lo cual, junto a los resultados de la receptividad estigmática y los ensayos preliminares de cruzamientos intra e interespecíficos, sugiere la existencia de protandría y autoincompatibilidad como barreras reproductivas que reducen la endogamia en estas especies.Reproductive phenology and flower anatomy of the plants Aloe vera and Aloe saponaria (Aloaceae in Cumana, Venezuela. The reproductive phenology and the floral anatomy of two species of Aloe kept under nursery conditions were analized in eastern Venezuela from September 2001 to September 2002. A. vera flowered between December and May; A. saponaria from November through August. In both species, the anthesis lasted 48 h., the anther dehiscence coincided with the opening of the perianth

  6. Identification of phytochemical components of aloe plantlets by gas ...

    African Journals Online (AJOL)

    MRT Pack 20 DVDs

    2013-12-04

    Dec 4, 2013 ... So, we can use this method (tissue culture) instead of aloe cultivation which is limited in some regions of the world. Key words: Aloe medicinal plant, phytochemical components, micropropagation, tissue culture, gas chromatography-mass spectrometry (GC-MS) analysis. INTRODUCTION. Aloe vera is a ...

  7. ANTIMICROBIAL ACTIVITY OF ROSA CANINA FLOWERS AGAINST SELECTED MICROORGANISMS

    Directory of Open Access Journals (Sweden)

    Katarína Rovná

    2015-02-01

    Full Text Available Rosa canina flowers were screened against various plant pathogenic microbial strains to study the antimicrobial properties of the plant. Ethanolic and methanolic extracts of flowers were screened applying agar well diffusion method against two Gram-negative bacteria including Escherichia coli CCM 3988 and Pseudomonas aeruginosa CCM 1960 and three microscopic filamentous fungi strains Aspergillus niger, Fusarium culmorum and Alternaria alternata, respectively. The best antimicrobial effect of ethanolic extract of Rosa canina flowers was found against Pseudomonas aeruginosa and the best antimicrobial effect of methanolic extract of Rosa canina flowers was found against Escherichia coli.

  8. Role of Natural Immunomodulator (Aloe Vera in Cellular and Humoral Immune Responses

    Directory of Open Access Journals (Sweden)

    Ening Wiedosari

    2007-12-01

    Full Text Available Aloe vera belongs to a group of Liliaceae family plant and cultivated worldwide. It possesses acemannan (acetylated mannan, which has a significant pharmacological property. The acemannan has an immunomodulatory activity when administered to animals. The major immunomodulating effect includes the activation of immune effector cells, such as lymphocytes and macrophages, resulting in the production of cytokines, interleukin (IL-1, IL-6, IL-12 and tumor necrosis factor alpha (TNFα. In particular, this extract can modulate the differentiation capacity of CD4+T cells to mature into Th1 subsets and enhance the innate cytokine response. As a consequence, this extract will have a profound effect in controlling disease, caused by intracellular infectious agents (bacteria and viruses. However, further studies are needed to determine the immunomodulating effects of Aloe vera in multi-component extracts equivalent to what are being used commonly in traditional medicine.

  9. ALOS-2 launch and initial checkout result

    Science.gov (United States)

    Arikawa, Yoshihisa; Kankaku, Yukihiro; Saruwatari, Hideki; Hatooka, Yasushi; Suzuki, Shinichi

    2014-10-01

    The Advanced Land Observing Satellite-2 (ALOS-2) carries the state-of-the-art L-band Synthetic Aperture Radar (SAR) called PALSAR-2 which succeeds to the ALOS / PALSAR. PALSAR-2 has an enhanced performance in both high resolution and wide swath compared to PALSAR. It will allow comprehensive monitoring of disasters. Wider bandwidth and shorter revisit time will give better conference for interferometry SAR (INSAR) data analysis such as crustal deformation and deforestation. ALOS-2 was launched on 24th May 2014, and has been completed the initial functional verifications of onboard components and systems. This paper describes the initial operation and checkout results including the comparison with the previous SAR satellite image and the disaster monitoring. Some key features of orbit control and determination to improve the coherency of the repeat-pass INSAR observation are evaluated.

  10. Caracterización morfoanatómica comparativa entre Aloe vera (L.) Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae)

    OpenAIRE

    Carpano, Stella Maris; Castro, María Teresa; Spegazzini, Etile Dolores

    2009-01-01

    Aloe vera (L.) Burm. F. (= Aloe barbadensis Miller) conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies d...

  11. Bio-prospecting endemic Mascarene Aloes for potential neuroprotectants.

    Science.gov (United States)

    Lobine, D; Howes, M-J R; Cummins, I; Govinden-Soulange, J; Ranghoo-Sanmukhiya, M; Lindsey, K; Chazot, P L

    2017-12-01

    The Mascarene Aloes are used in the traditional pharmacopoeia against various ailments including cutaneous diseases and as antispasmodics. Scientific evidence to support these claims is non-existent and mainly based on the scientific repute of A. vera. The antioxidant profile of methanolic leaf extracts of A. purpurea Lam., A. tormentorii (Marais) L. E. Newton & G. D. Rowley, A. lomatophylloides Balf. f., A. macra Haw. and A. vera (L.) Burm. f. was studied using the total antioxidant capacity, copper equivalent and superoxide dismutase assays. In vitro cytotoxicity was evaluated on CAD (Cath.-a-differentiated) neuronal cells by the methyl tetrazolium assay, and the neuroprotective profile was assessed using hydrogen peroxide-induced neurotoxicity with the CAD cells. The aloin and vitexin content were determined by high-performance liquid chromatography with diode-array detection. A. purpurea had the highest aloin content (546.6 nmol/g), while A. tormentorii had the highest vitexin content (67.3 nmol/g). A. macra (concentration aloin and vitexin content that are also present in other reputed medicinal Aloes. Copyright © 2017 John Wiley & Sons, Ltd.

  12. Clinical Effectiveness of Aloe Vera in the Management of Oral Mucosal Diseases- A Systematic Review

    Science.gov (United States)

    Nair, Gopakumar Ramachandran; Naidu, Giridhar Seetharam; Jain, Supreet; Makkad, Ramanpal Singh; Jha, Abhishek

    2016-01-01

    Introduction Aloe vera is well known for its medicinal properties which lead to its application in treating various diseases. Its use in treating oral lesions has not been much documented in literature. Aim Although, systematic reviews on aloe vera and its extracts have been done earlier, but in relation to oral diseases this is the first systematic review. The aim of the present systematic review was to compile evidence based studies on the effectiveness of Aloe vera in treatment of various oral diseases. Materials and Methods Computerized literature searches were performed to identify all published articles in the subject. The following databases were used: PUBMED [MEDLINE], SCOPUS, COCHRANE DATABASE, EMBASE and SCIENCE DIRECT using specific keywords. The search was limited to articles published in English or with an English Abstract. All articles (or abstracts if available as abstracts) were read in full. Data were extracted in a predefined fashion. Assessment was done using Jadad score. Results Fifteen studies satisfied the inclusion criteria. Population of sample study ranged from 20 patients to 110 patients with clinically diagnosed oral mucosal lesions. Out of 15 studies, five were on patients with oral lichen planus, two on patients with oral submucous fibrosis, other studies were carried on patients with burning mouth syndrome, radiation induced mucositis, candida associated denture stomatitis, xerostomic patients and four were on minor recurrent apthous stomatitis. Most studies showed statistically significant result demonstrating the effectiveness of Aloe vera in treatment of oral diseases. Conclusion Although there are promising results but in future, more controlled clinical trials are required to prove the effectiveness of Aloe vera for management of oral diseases. PMID:27656587

  13. Skin permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox.

    Science.gov (United States)

    Fox, Lizelle T; Gerber, Minja; du Preez, Jan L; du Plessis, Jeanetta; Hamman, Josias H

    2015-01-01

    The aim of this study was to investigate the in-vitro permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox using ketoprofen as a marker compound. The permeation studies were conducted across excised female abdominal skin in Franz diffusion cells, and the delivery of ketoprofen into the stratum corneum-epidermis and epidermis-dermis layers of the skin was investigated using a tape-stripping technique. A. vera gel showed the highest permeation-enhancing effect on ketoprofen (enhancement ratio or ER = 2.551) when compared with the control group, followed by A. marlothii gel (ER = 1.590) and A. ferox whole-leaf material (ER = 1.520). Non-linear curve fitting calculations indicated that the drug permeation-enhancing effect of A. vera gel can be attributed to an increased partitioning of the drug into the skin, while A. ferox whole leaf modified the diffusion characteristics of the skin for ketoprofen. The tape stripping results indicated that A. marlothii whole leaf delivered the highest concentration of the ketoprofen into the different skin layers. Of the selected aloe species investigated, A. vera gel material showed the highest potential as transdermal drug penetration enhancer across human skin. © 2014 Royal Pharmaceutical Society.

  14. Acemannan and Fructans from Aloe vera (Aloe barbadensis Miller) Plants as Novel Prebiotics.

    Science.gov (United States)

    Quezada, Maria Paz; Salinas, Carlos; Gotteland, Martin; Cardemil, Liliana

    2017-11-22

    The nutraceutical properties of Aloe vera have been attributed to a glucomannan known as acemannan. Recently information has been published about the presence of fructans in Aloe vera but there are no publications about acemannan and fructans as prebiotic compounds. This study investigated in vitro the prebiotic properties of these polysaccharides. Our results demonstrated that fructans from Aloe vera induced bacterial growth better than inulin (commercial FOS). Acemannan stimulated bacterial growth less than fructans, and as much as commercial FOS. Using qPCR to study the bacterial population of human feces fermented in a bioreactor simulating colon conditions, we found that fructans induce an increase in the population of Bifidobacterium spp. Fructans produced greater amounts of short chain fatty acids (SCFA), while the branched-chain fatty acids (BCFA) did not increase with these polysaccharides. Acemannan increased significantly acetate concentrations. Therefore, both Aloe vera polysaccharides have prebiotic potentials.

  15. Orchid flower evolution

    Indian Academy of Sciences (India)

    Unknown

    be explained by the ABC model of flower development and the polar coordinate model for zygomorphy which explains the phenotypic expression of flower symmetry in wild type and semipeloric flowers of Antirrhinum by the CYCLOIDEA, CYC gene. Similarly, the presence of such a similar gene in orchids CYCLOIDEA like, ...

  16. Design a Hummingbird Flower.

    Science.gov (United States)

    Bailey, Kim

    2002-01-01

    Presents an activity that engages students in designing and making an artificial flower adapted for pollination by hummingbirds. Students work in teams to design flowers that maximize the benefit from attracting hummingbirds. Examines characteristics of real flowers adapted to pollination by hummingbirds. (DLH)

  17. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Buerki, Sven; Symonds, Matthew RE

    2015-01-01

    Background: Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world’s largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between...... the rich traditions of medicinal use and leaf succulence in aloes. Results: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major...... radiations driven by different speciation processes, giving rise to the extraordinary diversity known today. Large, succulent leaves typical of medicinal aloes arose during the most recent diversification ~10 million years ago and are strongly correlated to the phylogeny and to the likelihood of a species...

  18. Meristem Culture of Aloe vera L

    International Nuclear Information System (INIS)

    Kay Thi Mya; Thida Myint; Myo Han; Khin Maung Sein

    2005-10-01

    Shoot tips of Aloe vera L were excised and inoculated in basal and modified MS (Murashige and Skoog, 1962) medium. Growth regulators and growth factors were supplemented to the basal medium. Development of explants, initiation of callus and multiplication of shoots in culture were recorded.

  19. Hypolipidemic, Hypoglycemic and Antioxidant Activities of Flower ...

    African Journals Online (AJOL)

    Purpose: To investigate the anti-dyslipidemic, anti-oxidant and anti-diabetic activities of the aqueous extract and solvent fractions of A. violacea flowers. Methods: The aqueous extract was fractionated into petroleum ether, ether, chloroform, chloroformmethanol (4:1) and chloroform-methanol (3:2) fractions. Lipid lowering ...

  20. Dermocosmetics for dry skin: a new role for botanical extracts.

    Science.gov (United States)

    Casetti, F; Wölfle, U; Gehring, W; Schempp, C M

    2011-01-01

    Dry skin is associated with a disturbed skin barrier and reduced formation of epidermal proteins and lipids. During recent years, skin-barrier-reinforcing properties of some botanical compounds have been described. Searching the PubMed database revealed 9 botanical extracts that specifically improve skin barrier and/or promote keratinocyte differentiation in vivo after topical application. The topical application of Aloe vera (leaf gel), Betula alba (birch bark extract), Helianthus annuus (sunflower oleodistillate), Hypericum perforatum (St. John's wort extract), Lithospermum erythrorhizon (root extract), Piptadenia colubrina (angico-branco extract) and Simarouba amara (bitter wood extract) increased skin hydration, reduced the transepidermal water loss, or promoted keratinocyte differentiation in humans in vivo. The topical application of Rubia cordifolia root extract and rose oil obtained from Rosa spp. flowers stimulated keratinocyte differentiation in mouse models. The underlying mechanisms of these effects are discussed. It is concluded that some botanical compounds display skin-barrier-reinforcing properties that may be used in dermocosmetics for dry skin. However, more investigations on the mode of action and more vehicle-controlled studies are required. Copyright © 2011 S. Karger AG, Basel.

  1. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera.

    Science.gov (United States)

    Grace, Olwen M; Buerki, Sven; Symonds, Matthew R E; Forest, Félix; van Wyk, Abraham E; Smith, Gideon F; Klopper, Ronell R; Bjorå, Charlotte S; Neale, Sophie; Demissew, Sebsebe; Simmonds, Monique S J; Rønsted, Nina

    2015-02-26

    Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world's largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between the rich traditions of medicinal use and leaf succulence in aloes. The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major radiations driven by different speciation processes, giving rise to the extraordinary diversity known today. Large, succulent leaves typical of medicinal aloes arose during the most recent diversification ~10 million years ago and are strongly correlated to the phylogeny and to the likelihood of a species being used for medicine. A significant, albeit weak, phylogenetic signal is evident in the medicinal uses of aloes, suggesting that the properties for which they are valued do not occur randomly across the branches of the phylogenetic tree. Phylogenetic investigation of plant use and leaf succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue, an adaptive feature that likely contributed to the ecological success of the genus Aloe, is the main predictor for medicinal use among Aloe species, whereas evolutionary loss of succulence tends to be associated with losses of medicinal use. Phylogenetic analyses of plant use offer potential to understand patterns in the value of global plant diversity.

  2. In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications

    Science.gov (United States)

    Fox, Lizelle T.; du Plessis, Jeanetta; Gerber, Minja; van Zyl, Sterna; Boneschans, Banie; Hamman, Josias H.

    2014-01-01

    Objective: To investigate the skin hydrating and anti-erythema activity of gel materials from Aloe marlothii A. Berger and A. ferox Mill. in comparison to that of Aloe barbadensis Miller (Aloe vera) in healthy human volunteers. Materials and Methods: Aqueous solutions of the polisaccharidic fractions of the selected aloe leaf gel materials were applied to the volar forearm skin of female subjects. The hydration effect of the aloe gel materials were measured with a Corneometer® CM 825, Visioscan® VC 98 and Cutometer® dual MPA 580 after single and multiple applications. The Mexameter® MX 18 was used to determine the anti-erythema effects of the aloe material solutions on irritated skin areas. Results: The A. vera and A. marlothii gel materials hydrated the skin after a single application, whereas the A. ferox gel material showed dehydration effects compared to the placebo. After multiple applications all the aloe materials exhibited dehydration effects on the skin. Mexameter® readings showed that A. vera and A. ferox have anti-erythema activity similar to that of the positive control group (i.e. hydrocortisone gel) after 6 days of treatment. Conclusion: The polysaccharide component of the gel materials from selected aloe species has a dehydrating effect on the skin after multiple applications. Both A. vera and A. ferox gel materials showed potential to reduce erythema on the skin similar to that of hydrocortisone gel. PMID:24991119

  3. Aggregation of Thaumatomyia glabra (Diptera: Chloropidae) Males on Iris spp. Flowers Releasing Methyl Anthranilate.

    Science.gov (United States)

    Ohler, Bonnie J; Guédot, Christelle; Zack, Richard S; Landolt, Peter J

    2016-12-01

    Aggregations of Thaumatomyia glabra (Diptera: Chloropidae) were observed on flowers of Iris pallida Lamarck (Asparagales: Iridaceae), whereas no T. glabra (Meigen) were observed on nearby Iris germanica L. flowers. Sampling of T. glabra on I. pallida flowers revealed the presence of males only. In a previous study, T. glabra males were attracted to methyl anthranilate. We found methyl anthranilate in extracts of I. pallida flowers on which T. glabra aggregated, but not in extracts of I. germanica flowers. Applying methyl anthranilate to I. germanica flowers elicited attraction of T. glabra to the flowers. This study suggests that I. pallida flowers may attract T. glabra males to aggregate because they release the known attractant, methyl anthranilate, whereas I. germanica flowers may not be attractive because they do not release methyl anthranilate. Published by Oxford University Press on behalf of Entomological Society of America 2016. This work is written by US Government employees and is in the public domain in the US.

  4. EVALUATION OF FREEZE DRIED ALOE VERA AND NOPAL CACTUS FOR POSSIBLE HEALTH TREATMENTS BY COMPARISON OF ANTIOXIDANT PROPERTIES AND FREE RADICAL INHIBITION.

    Science.gov (United States)

    Rodriguez, Kenneth R; Jones, Anthony E; Belmont, Barbara

    2014-01-01

    The goal of this project was to characterize the antioxidant powers of lyophilized Aloe Vera ( Aloe barbadensis ) and Nopal Cactus (Opuntia ficus-indica) by quantifying the phenolics content and radical scavenging abilities of preparations derived from these plants. Extracts of these lyophylized succulents were assayed for phenolic compounds by the Folin Ciocalteau method and compared for free radical scavenging capability by the DPPH method. We found that even though the Aloe lyophilizate extract contained more phenolic content, the Nopal lyophilizate exhibited better free radical scavenging ability. Aloe Vera extract contained 0.278 g/L of phenolic content and exhibited 11.1% free radical inhibition, with a free radical scavenging rate constant of 0.177±0.015 min -1 . Nopal Cactus extract contained 0.174 g/L of phenolic content and exhibited 13.2% free radical inhibition, with a free radical scavenging rate constant of 0.155±0.009 min -1 . These results showed Nopal to have greater antioxidant potency than Aloe.

  5. Aloe vera-based formula as emollient on horses' hooves

    Directory of Open Access Journals (Sweden)

    Samuel Pereira Simonato

    2013-06-01

    Full Text Available The present study aimed at developing an Aloe vera-based formula for topical use on horse hoof and evaluating whether the treatment affects hooves growth and balance. Six healthy male horses between the ages of 3 and 17 years (12±5.25 were used, all semi-confined animals for breeding purposes. Before beginning A. vera treatment, animals underwent two trimming procedures with a 45 days-interval. After the second trimming, one of the forelimbs and one of the hindlimbs of 4 horses was weekly treated by topical application of the glycolic extract of A. vera at 20%. The contralateral limb, randomly chosen, received the extract at 50%. The hooves of the other animals were treated with propylene glycol. Treatment was done for 225 days and, during this time, animals underwent periodic trimming. Variables related to growth and balance of the hooves were measured before and after trimming. Data were analyzed using chi-square test and regression analysis at 5% significance. Growth rate of the hooves was not related to treatment. On the other hand, the 50% extract was related to the majority of the hooves in balance (p<0.05. Results suggest that a weekly topical treatment with A. vera glycolic extract does not improve the growth rate of the hooves; however, when applied at a high concentration, it improves their balance.

  6. Aloe vera for treating acute and chronic wounds

    OpenAIRE

    Dat,Anthony D.; Poon,Flora; Pham,Kim B. T.; Doust,Jenny

    2014-01-01

    BACKGROUND: Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process.OBJECTIVE: To determine the effects of Aloe vera-derived products (for example dressings and topical gels) on the healing of acute wounds (for example lacerations, surgical incisions and burns) and chronic wounds (for example infected wounds, arterial and venous ulcer...

  7. [Determination of aloin in aloes by HPLC].

    Science.gov (United States)

    Cao, Hong; Liu, Yun

    2003-04-01

    To describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes. The analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm. The calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6). The results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin.

  8. A revised generic classification for Aloe (Xanthorrhoeaceae subfam. Asphodeloideae)

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Klopper, Ronell R.; Smith, Gideon F.

    2013-01-01

    The predominantly southern African Xanthorrhoeaceae subfam. Asphodeloideae Asphodelaceae subfam. Alooideae) has long been regarded as comprising seven so-called alooid genera (Aloe, Astroloba, Chortolirion, Gasteria, Haworthia, Lomatophyllum, Poellnitzia). A reassessment of the classification...... of the traditionally broadly circumscribed genus Aloe, a charismatic Old World group of leaf succulents, has necessitated nomenclatural adjustments. We propose a narrower generic concept for Aloe s. str. and the recognition of segregate genera to reflect accumulating evidence for monophyletic groups: here, the genus...... Kumara is reinstated and the new genera Aloidendron and Aloiampelos are established. New combinations are made in Aloe for the three species of Chortolirion....

  9. Unraveling the medicinal potential of South African Aloe species.

    Science.gov (United States)

    Amoo, Stephen O; Aremu, Adeyemi O; Van Staden, Johannes

    2014-04-11

    Aloe species (family: Xanthorrhoeaceae) are widely used in ethnomedicine for treating various ailments. Approximately 125 Aloe taxa are documented as indigenous to South Africa. This review was aimed at critically evaluating the available data on the ethnomedicinal uses, pharmacological activities, safety and conservation status of the documented South African Aloe species. A detailed search using major electronic search engines (such as Google Scholar, Scopus and Scirus) and ethnobotanical literature was undertaken. Search terms used included 'medicinal properties of Aloe species', 'biological activity of South African Aloe species' and 'safety and toxicological evaluation of aloes'. Although Aloe species are widely used as laxatives and for treating wound and skin-related ailments, only about 20% of South African Aloe species have been clearly documented for their medicinal uses. The pharmacological potential including the antimicrobial, antiinflammatory, antiplasmodial and anthelmintic activities of some of the species has been established, providing a rationale for their use in traditional medicine. Successful micropropagation protocols have been developed as a conservation strategy, but only for a few species. The highlighted medicinal activities of some Aloe species indicate their therapeutic potential. Nonetheless, further research especially on the understudied species is required to properly document their ethnomedicinal uses and fully explore their pharmacological value. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  10. Caracterización morfoanatómica comparativa entre Aloe vera (L. Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae

    Directory of Open Access Journals (Sweden)

    Stella Maris Carpano

    Full Text Available Aloe vera (L. Burm. F. (= Aloe barbadensis Miller conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies do mesmo gênero. Isto é o que ocorre com três espécies de Aloe spp., que se cultivam ou crescem acidentalmente na Argentina: A. arborescens Mill., A. saponaria Haw. e A. ciliaris Haw. (Aloaceae. Estabeleceram-se os caracteres morfoanatômicos de diagnóstico por microscopia óptica e microscopia eletrônica de varredura (apresentam-se desenhos e fotomicrografias dos mesmos. Obtiveram-se valores numéricos dos elementos histológicos de diagnóstico: magnitudes lineares e proporcionais. Realizaram-se reações histoquímicas de identificação dos princípios ativos para sua localização "in situ".

  11. Formulation development, optimization and evaluation of aloe vera gel for wound healing.

    Science.gov (United States)

    Khan, Abdul Wadood; Kotta, Sabna; Ansari, Shahid Hussain; Sharma, Rakesh Kumar; Kumar, Amit; Ali, Javed

    2013-10-01

    To formulate and optimize a herbal gel of Aloe vera extract containing Carbopol 934 as gelling agent and to investigate the effects of topical application of Carbopol 934 gel containing Aloe vera extract on the healing of skin wounds surgically induced in Wistar rats. Different concentrations of viscosity enhancer Carbopol 934 were tried and finally gel that showed good spreadability and consistency was selected for wound healing property of herbal gel of Aloe vera. Excision wound model was used for the study. The optimized gel was evaluated for different physicochemical properties and wound healing property. Differences in wound healing were observed between the various treatments when compared to the control group. Tissue hyperplasia was lower in the control group compared to the other treated groups. In animals group treated with gel, 80.14% healing was observed up to 14(th) day. While in untreated group I (control) animals showed 52.68% healing of wounds on 14(th) day. On the other hand, control group animals also showed inflammation and pus formation up to 5(th) day of study, while treated animals did not showed any observable inflammation and pus formation. Results shows prepared gel has promising effect on the wound healing process.

  12. Impedance studies of a green blend polymer electrolyte based on PVA and Aloe-vera

    Science.gov (United States)

    Selvalakshmi, S.; Mathavan, T.; Vijaya, N.; Selvasekarapandian, Premalatha, M.; Monisha, S.

    2016-05-01

    The development of polymer electrolyte materials for energy generating and energy storage devices is a challenge today. A new type of blended green electrolyte based on Poly-vinyl alcohol (PVA) and Aloe-vera has been prepared by solution casting technique. The blending of polymers may lead to the increase in stability due to one polymer portraying itself as a mechanical stiffener and the other as a gelled matrix supported by the other. The prepared blend electrolytes were subjected to Ac impedance studies. It has been found out that the polymer film in which 1 gm of PVA was dissolved in 40 ml of Aloe-vera extract exhibits highest conductivity and its value is 3.08 × 10-4 S cm-1.

  13. Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge.

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; Ma, Xin Yu; He, Jie; Xu, Pao; Liu, Kai

    2015-10-01

    The current study investigated the effects of dietary Aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of GIFT-tilapia juveniles under Streptococcus iniae challenge. Five dietary groups were designed including a control and 100 % Aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. Fish fed dietary Aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced (P Aloe/kg feed compared to unsupplemented ones. High-density lipoprotein was significantly elevated in fish fed 0.5 and 1 % Aloe/kg feed compared to unsupplemented ones, and no significant changes (P > 0.05) were noted in low-density lipoprotein among test groups. Furthermore, high activities of superoxide dismutase, catalase, and glutathione peroxide in liver tissues were observed in Aloe-supplemented fish compared to unsupplemented ones, before and after S. iniae challenge (7.7 × 10(6) CFU cells/mL). Variations were also noted in malondialdehyde activity throughout the trial, but no significant difference (P > 0.05) was observed between groups. Meanwhile, Aloe-supplemented fish reduced serum aspartate and alanine aminotransferase (AST and ALT) activities before and after challenge. Based on the second-order polynomial regression analysis, dietary Aloe inclusion levels less than or equal to 1.88, 1.86, and 2.79 %/kg feed were determined to be suitable in improving plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia in this study, respectively. Thus, A. vera extracts may be recommended as a tilapia feed supplement to enhance fish antioxidant and hepatoprotective capacities, especially during disease outbreaks.

  14. Involvement of Vanilloid Receptors in the Anti-inflammatory Effect of Hydroalcoholic Extract of Heated Female Cannabis sativa Flowers in Rat

    Directory of Open Access Journals (Sweden)

    Masoud Fereidoni

    2016-06-01

    Full Text Available Background and Objectives: Decarboxylated phytocannabinoids activate endocannabinoid system in the central nervous system and peripheral tissues via activation of cannabinoid receptors. In this investigation, the effect of interaction between decarboxylated phytocannabinoids and capsaicin at the central level and naloxone at the systemic level, was investigated on inflammatory rat paw edema induced by by sub-plantar injection. Methods: In this experimental study, male Wistar rats (200-250g were used. To study the effect of interaction with opioid system, intraperitoneal administration of hydroalcoholic extract (dose, 50mg/kg, naloxone (dose, 2mg/kg and co-administration of the extract and naloxone were performed. To investigate the effect of interaction with vanilloid system, intrathecal administration of extract (0.01mg/10μl, capsaicin (0.002mg/10μl and co-administration of extract and capsaicin were performed. Paw volume was measured before and one hour after sub-plantar administration of formalin using plethysmometer method in order to assess inflammatory edema. Results: Intraperitoneal administration of extract alone or in combination with naloxone reduced the inflammatory rat paw edema volume (p<0.001. On the other hand, capsaicin increased rat paw edema volume (p<0.01, but intrathecal administration of extract alone and in combination with capsaicin decreased the inflammatory rat paw edema volume (p<0.01. Conclusion: The results of this study showed that decarboxylated cannabinoids in hydroalcoholic extract decrease the capsaicin-induced inflammatory rat paw edema through activation of CB1 and CB2 receptors and desensitization of TRPV1 receptor. On the contrary, administration of naloxone has not antagonized the reduction effect of extract on inflammatory paw edema, and it is likely that the effect of the extract has not exerted through opioid receptors.

  15. In vitro activity of Aloe vera inner gel against Helicobacter pylori strains.

    Science.gov (United States)

    Cellini, L; Di Bartolomeo, S; Di Campli, E; Genovese, S; Locatelli, M; Di Giulio, M

    2014-07-01

    Aloe barbadensis Miller (Aloe vera) is a herbal remedy widely used for a variety of illnesses; A. vera leaf extracts have been promoted for detoxification, cure constipation, help flush out toxins and wastes from the body, promote digestion and are used in the treatment of peptic ulcer for cytoprotective action. The aim of this study was to evaluate the antibacterial activity of A. vera inner gel against both susceptible and resistant Helicobacter pylori strains isolated in Abruzzo region, Italy. The inner gel of leaves of a 5-year-old plant of A. vera was extracted, homogenized and tested from 800 to 1.56 mg ml(-1) against 14 clinical strains and one reference strain of H. pylori using the broth microdilution methodology. Furthermore, the sample of A. vera was investigated for the chemical fingerprint of anthraquinones. The inhibitory concentrations of A. vera inner gel were similar to the bactericidal ones, with values ranging from 6.25 to 800 mg ml(-1) . Fifty per cent of the detected strains, independently of their susceptibility profile, were inhibited in their growth at 100 mg ml(-1) . Aloe vera inner gel expresses antibacterial properties against H. pylori and, therefore, in combination with antibiotics, could represent a novel strategy for the treatment of the infection of H. pylori, especially in cases of multiresistance. The study demonstrates that the Aloe vera inner gel expresses antibacterial properties against both susceptible and resistant Helicobacter pylori strains. These findings may impact on the antimicrobial resistance phenomenon of H. pylori, proposing the A. vera inner gel as a novel effective natural agent for combination with antibiotics for the treatment of H. pylori gastric infection. © 2014 The Society for Applied Microbiology.

  16. Three-Arm Randomized Phase III Trial: Quality Aloe and Placebo Cream Versus Powder as Skin Treatment During Breast Cancer Radiation Therapy.

    Science.gov (United States)

    Hoopfer, Donna; Holloway, Caroline; Gabos, Zsolt; Alidrisi, Maha; Chafe, Susan; Krause, Barbara; Lees, Alan; Mehta, Nirmal; Tankel, Keith; Strickland, Faith; Hanson, John; King, Charlotte; Ghosh, Sunita; Severin, Diane

    2015-06-01

    The efficacy of aloe extract in reducing radiation-induced skin injury is controversial. The purpose of the present 3-arm randomized trial was to test the efficacy of quality-tested aloe extract in reducing the severity of radiation-induced skin injury and, secondarily, to examine the effect of a moist cream versus a dry powder skin care regimen. A total of 248 patients with breast cancer were randomized to powder, aloe cream, or placebo cream. Acute skin toxicity was scored weekly and after treatment at weeks 1, 2, and 4 using a modified 10-point Catterall scale. The patients scored their symptom severity using a 6-point Likert scale and kept an acute phase diary. The aloe formulation did not reduce acute skin toxicity or symptom severity. Patients with a greater body mass index were more likely to develop acute skin toxicity. A similar pattern of increased skin reaction toxicity occurred with both study creams compared with the dry powder regimen. No evidence was found to support prophylactic application of quality aloe extract or cream to improve the symptoms or reduce the skin reaction severity. Our results support a dry skin care regimen of powder during radiation therapy. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. The Foliar Anatomy And Micromorphology Of Aloe Ferox Mill ...

    African Journals Online (AJOL)

    Background: The genus Aloe has been the subject of several chemotaxonomic and phytochemical investigations. Aloe ferox is an important source of biologically active compounds like anthrones, chromones, pyrones, and flavonoids. It is a plant used extensively in traditional medicine. Despite the myriad of studies on the ...

  18. Fast Dissolving Tablets of Aloe Vera Gel | Madan | Tropical Journal ...

    African Journals Online (AJOL)

    Purpose: The objective of this work was to prepare and evaluate fast dissolving tablets of the nutraceutical, freeze dried Aloe vera gel. Methods: Fast dissolving tablets of the nutraceutical, freeze-dried Aloe vera gel, were prepared by dry granulation method. The tablets were evaluated for crushing strength, disintegration ...

  19. Distribution, diversity and conservation of the genus Aloe in Kenya ...

    African Journals Online (AJOL)

    The genus Aloe is common in Kenya, with about 60 taxa recognised. Observations from this study indicate that most of the taxa have a restricted distribution, with only Aloe secundiflora var. secundiflora being widespread in the country. The diversity patterns indicate a high concentration of taxa in three areas that are ...

  20. Immunohistochemical studies on the effect of Aloe vera on the ...

    African Journals Online (AJOL)

    Using n0-STZ type-II diabetic rats, the immunoreactivity of β-cells of the islets of Langerhans did not differ among treatments of control, glibenclamide-, Aloe vera leaf pulp- and ... KEYWORDS: Aloe vera, type-II diabetes, pancreatic β-cells, immunohistochemistry ... the Herbarium of Istanbul University, Faculty of Pharmacy.

  1. Arizona Lesson Observation and Evaluation (ALOE): Design Test Edition, 1990.

    Science.gov (United States)

    Fargo, J. Steven; And Others

    The Arizona Lesson Observation and Evaluation (ALOE) system of evaluating teaching is presented. ALOE was developed from Arizona adaptations of the Georgia Teacher Performance Assessment Instruments and, with G. Taylor's "Functional Elements Analysis of Teaching Skills" (FEATS), forms an integrated observation package which allows…

  2. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress.

    Science.gov (United States)

    Salinas, Carlos; Handford, Michael; Pauly, Markus; Dupree, Paul; Cardemil, Liliana

    2016-01-01

    Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC). There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC) in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

  3. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera Grown under Water Stress.

    Directory of Open Access Journals (Sweden)

    Carlos Salinas

    Full Text Available Aloe barbadensis Miller (Aloe vera has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC. There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

  4. Evaluation of aloin and aloe-emodin as anti-inflammatory agents in aloe by using murine macrophages.

    Science.gov (United States)

    Park, Mi-Young; Kwon, Hoon-Jeong; Sung, Mi-Kyung

    2009-04-23

    The aloe ingredients responsible for physiological effects and the concentrations required to exert their biological activities are not fully understood. This study compares the anti-inflammatory effects of aloin and aloe-emodin with other polyphenols. Our results demonstrated that aloe-emodin dose-dependently inhibited inducible nitric oxide synthase (iNOS) mRNA expression and nitric oxide (NO) production at 5-40 microM. In addition, the levels of cyclooxygenase-2 (COX-2) mRNA and prostaglandin E2 (PGE2) production were suppressed by 40 microM aloe-emodin. Aloin also suppressed the production of NO at 5-40 microM, although it did not suppress PGE2 production. The present results indicate that aloin and aloe-emodin possibly suppress the inflammatory responses by blocking iNOS and COX-2 mRNA expression. The anti-inflammatory effect of aloe-emodin was comparable to that of kaempferol and quercetin, indicating aloe-emodin as a possible key constituent responsible for the anti-inflammatory activity of aloe.

  5. Phytochemical screening of the exudate of Aloe otallensis and its effect on Leishmania donovani

    Directory of Open Access Journals (Sweden)

    Zerihun Tesfaye Nigusse

    2016-06-01

    Full Text Available Objective: To evaluate the antileishmanial activity of methanolic extract of Aloe otallensis (A. otallensis on the promastigote stage of Leishmania donovani (L. donovani as compared to standard drugs and to screen its phytochemical constituents. Methods: Phytochemical screening was done by using the method mentioned by Evans and Trease on methanolic extract of the exudates of Aloe otallensis leaves. The extract was also evaluated for in vitro antileishmanial activity against L. donavani which is found from the Parasitology Unit of Black Lion Hospital. The result was compared to standard drugs of sodium stibogluconate, milfostin and paramomycin. Results: The extract has a good antileishmanial activity with an IC50 of 0.1230 μg/mL on L. donovani (AM 563. The experimental data showed that relatively it had better activity than paramomycin and milfostin but less activity than sodium stibogluconate. The data analyses were done by GraphPad Prism version 5 software after it was read by ELISA reader at the wave length of 650 nm. The phytochemical screening of the exudates of A. otallensis showed the presence of phenol, alkaloid and saponin. Conclusions: The methanol extract of the exudates of A. otallensis has a good antileishmaniasis activity and this may be attributed to phenol, alkaloid and saponin present in the plant. But it needs further analysis for the conformation of which constituent presents in high concentration to know which one has the strongest effect.

  6. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

    Directory of Open Access Journals (Sweden)

    Pushkala Ramachandran

    2014-01-01

    Full Text Available Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG and papaya. Aloe gel (30%, papaya pulp (15%, spice extract (5%, and citric acid (0.1% were mixed in given proportion to prepare the blend with TSS of 15 °Brix. The product was bottled, pasteurized, and stored at room temperature. The quality characteristics and storage stability of the spiced beverage blend (SAGPB were compared with spiced papaya RTS beverage (SPB. Periodic analysis was carried out up to five months for various physicochemical parameters, sugar profile, bioactive compounds, microbial quality, instrumental color, and sensory acceptability. The SAGPB exhibited superior quality characteristics compared to SPB both in fresh and in stored samples. The SPB was acceptable up to four months and SAGPB for five months. The results indicate that nutraceutical rich AG could be successfully utilized to develop functional fruit beverages with improved quality and shelf life.

  7. Antioxidant and Cytotoxicological Effects of Aloe vera Food Supplements

    Directory of Open Access Journals (Sweden)

    Zaira López

    2017-01-01

    Full Text Available Currently, food industries use supplements from Aloe vera as highly concentrated powders (starting products, which are added to the final product at a concentration of 1x, meaning 10 g/L for decolourized and spray-dried whole leaf powder (WLP or 5 g/L for decolourized and spray-dried inner leaf powder (ILG and also for nondecolourized and belt-dried inner leaf powder (ILF. Flavonoids, tannins, or saponins could not be detected for any starting product at this concentration and their total phenol concentration of 68–112 μM gallate-eq. was much lower than in fresh extract; however, their antioxidant capacity of 90–123 μM ascorbate-eq. for DPPH was similar to the fresh extract. Starting products, dissolved at 1x, had an aloin concentration of 0.04 to 0.07 ppm, a concentration much lower than the industry standard of 10 ppm for foodstuff. While decolourized starting products (i.e., treated with activated carbon exhibited low cytotoxicity on HeLa cells (CC50 = 15 g/L ILG or 50 g/L WLP, ILF at CC50 = 1–5 g/L exhibited cytotoxic effects, that is, at concentrations even below the recommended for human consumption. Probable causes for the cytotoxicity of ILF are the exposure to high temperatures (70–85°C combined with a high fibre content.

  8. Translating BPEL to FLOWer

    DEFF Research Database (Denmark)

    Lassen, Kristian Bisgaard

    FLOWer is a case handling tool made by Pallas-Athena for process management in the service industry. BPEL on the other hand is a language for web service orchestration, and has become a de facto standard, because of its popularity, for specifying workflow processes even though that was not its...... original purpose. This paper describe an approach translating BPLE to FLOWer, or more precisely form BPEL to CHIP. where CHIP is the interchange language that FLOWer import from and export to. The aim of the translation scheme that I give is to derive a CHIP specification that is behaviorally equivalent...

  9. High-performance nanothermite composites based on aloe-vera-directed CuO nanorods.

    Science.gov (United States)

    Patel, Vinay Kumar; Bhattacharya, Shantanu

    2013-12-26

    In this work, we demonstrate the development of high-performance nanothermite composites derived from super-reactive CuO nanorods oxidizers fabricated by simple biogenic routes using Aloe vera plant extracts. Nanorods of various length scales have been realized via simple sonoemulsion and solid-state biosynthesis routes using Aloe vera gel as a green surfactant promoting the directional growth of CuO nanorods in both solid and emulsion phase. The biosynthesized CuO nanorods (oxidizers)/fuel (nanoaluminum) composites ignited vigorously with abundant gas generation, developing high heat of reaction of 1.66 kJ g(-1) and very high pressurization rate of around 1.09 MPa μs(-1) and peak pressure of 65.4 MPa when blasted inside a constant volume pressure cell with a charge density of 0.2 g cm(-3). The pressurization rates so obtained are four times higher with twice the peak pressure in comparison to such nanothermites formulated via other available state of the art wet-chemical techniques, which reflects the catalytic role of Aloe vera surface functional groups (A. vera-sfg) enhancing the reactivity of CuO oxidizers with excess gas release rate during exothermic reaction with nanoaluminum. Through this work, Aloe vera gel has for the first time been identified as a novel biotemplate for green synthesis of nanorod structures of metal oxides, and we have also studied the utility of A. vera-sfg in the creation of super-reactive CuO oxidizers producing excellent heat of reaction and dynamic pressure characteristics as demanded in propellants, explosives, and pyrotechnics.

  10. Evaluation of the combined effect of St John's wort hydroethanolic flower extract and flaxseed oil on skin wound healing -in rats-

    Directory of Open Access Journals (Sweden)

    mohammadreza farahpour

    2014-05-01

    Full Text Available The use of biological substances is among the methods utilized to increase the rate and quality of wound healing. This experiment was designed to investigate the effects of co-administration of St John's wort hydroethanolic extract and flaxseed oil for acceleration of wound healing based on their therapeutic properties. The study was conducted on 72 male Wistar rats. Experimental wounds measuring 7 mm in diameter was created on both sides of animals then the animals treated with ointments once a day for 2 consecutive weeks. Wound area was determined on days 2, 4, 6, 8, 10, 12 and 14 using a transparent paper. Tissue samples were collected on days 3, 7 and 14 for histopathological studies. According to the data, hydroethanolic extract of St John's wort and flaxseed oil ointment decreased wound area and distribution of polymorphonuclear immune cells in wound area as well as increase in new vessel formation and fibroplasia (P

  11. Stars and Flowers, Flowers and Stars

    Science.gov (United States)

    Minti, Hari

    2012-12-01

    The author, a graduated from the Bucharest University (1964), actually living and working in Israel, concerns his book to variable stars and flowers, two domains of his interest. The analogies includes double stars, eclipsing double stars, eclipses, Big Bang. The book contains 34 chapters, each of which concerns various relations between astronomy and other sciences and pseudosciences such as Psychology, Religion, Geology, Computers and Astrology (to which the author is not an adherent). A special part of the book is dedicated to archeoastronomy and ethnoastronomy, as well as to history of astronomy. Between the main points of interest of these parts: ancient sanctuaries in Sarmizegetusa (Dacia), Stone Henge(UK) and other. The last chapter of the book is dedicated to flowers. The book is richly illustrated. It is designed for a wide circle of readers.

  12. Hypoglycemic effect of instant aloe vera on the diabetic rats

    Directory of Open Access Journals (Sweden)

    Riyanto

    2017-09-01

    Full Text Available Instant aloe vera contains phenolic compounds which has antioxidative activity. However, this product is hygroscopic and damaged easily during storage. The critical condition of the instant occurs at the moisture content of 12.52 ± 0.24% (wb. Increasing the moisture content could accelerate oxidation of the phenolic compounds, thus decrease the antioxidative activity. Previous research showed that the antioxidative activity of instant aloe vera could lower the blood glucose. The purpose of this study was to evaluate the hypoglycemic activity of instant aloe vera during storage until the critical condition. The hypoglycemic effect was determined with the in vivo method using diabetic Wistar rats as experimental animals. The diabetic rats were fed with a standard feed combined with instant aloe vera which has been stored at various storage time i.e. 0, 2, 4, 6, 8 weeks and used normal rats fed without instant aloe vera as a control. The blood glucose was analyzed every week until 4 weeks. The research showed that the diabetic rats fed with standard feed without instant aloe vera had high blood glucose (219.40 mg/dL after 4 weeks treatment. Otherwise, the blood glucose of diabetic rats fed with instant aloe vera decreased from 214.00 mg/dL to 97.57 mg/dL after 4 weeks.

  13. Antihemolytic activity and mineral contents of Juglans regia L. flowers.

    Science.gov (United States)

    Ebrahimzadeh, M A; Nabavi, S F; Nabavi, S M

    2013-07-01

    Juglans (J.) regia L. is known to possess many biological properties. In this study, antihemolytic activity of methanol extract of Juglans regia L. flower were investigated. Antihemolytic activities of Juglans regia L. flowers were evaluated by various in vitro assays. In addition, scavenging of hydrogen peroxide and mineral contents of flowers were determined using atomic absorption spectroscopy. Extract showed good antihemolytic activity against H2O2 and CuOOH induced hemolysis in comparison with control. Extract was capable of scavenging H2O2 in a concentration dependent manner. IC50 for H2O2 scavenging activity was 311±12.8 µg ml-1. The amount of eight elements was determined and was in the order: Mn > Cu > Fe > Zn. Our study indicate that J. regia flower has remarkable antihemolytic activity, which maybe result of its high phenol and flavonoid contents, especially quercetin.

  14. Aloe vera Leaf Anti Inflamation’s Activity Speeds Up the Healing Proccess of Oral Mucosa Ulceration

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    Agoeng Tjahajani

    2013-05-01

    Full Text Available Previous work showed that 25% of inner layer Aloe vera leaf extract was effective as anti-inflammatory on the oral mucous of Wistar rats. Objective: The aim of this study was to examine the lowest concentration of whole leaf Aloe vera extract that could accelerate healing process of ulceration on rats. Methods: Sixty inbreeding Spraque Dawley rats was exposed to solution of Hydrogen Peroxide 10% during three days to induced inflammation on the labial mucous inferior of rats. During the next three days, on the same area of control group were topically applied solution of Natrium Chloride 0.9%; while in the treatment group were topically applied 6.25%; 12.5%; 25% of whole leaf of Aloe vera extract for 3x5 minutes with 90 minutes intervals. On the fourth day, five rats in each group were sacrificed; and the same treatment was done on others groups. On the sixth day, five rats of each group were sacrificed; and the same treatment was done on the rest group up to seven days. On the eighth days, the rest groups were sacrificed. Microscopic slides were done. Results: Microscopic slides were analyzed under light microscope and scored. Statistical analysis with Mann-Whitney test showed significant differences between control and treatment group (p<0.05. Conclusion: Application of 6.25% whole leaf Aloe vera extract was the lowest and the most effective concentration in accelerating the healing process of oral mucous ulceration on rats.DOI: 10.14693/jdi.v18i1.56

  15. Efficacy of Calendula officinalis Extract (Marigold Flower as an Antimicrobial Agent against Oral Microbes: An Invitro Study in Comparison with Chlorhexidine Digluconate

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    Shrinidhi Maji Shankar

    2017-10-01

    Full Text Available Introduction: Calendula officinalis is a member of the family Asteraceae containing flavonoids, and essential oils etc. It is known to be effective against certain gram negative and gram positive clinical pathogens. Incorporating natural plant extracts into periodontal antiinfective therapy is a wise alternative in light of rampant antibiotic resistance amongst periodontal pathogens. Aim: To assess the antimicrobial efficacy of C.officinalis against five oral microbes, as compared to gold standard chlorhexidine digluconate. Materials and Methods: The inhibitory action of the two test agents, chlorhexidine digluconate 0.2% and C.officinalis raw extract was assessed using tests to determine minimum inhibitory concentration, minimum bactericidal concentration, and inhibition zones using agar diffusion method. Time kill curve method was used to assess the time in which drug was inhibitive against five oral microbes namely, A.actinomycetemcomitans, P.gingivalis, P.intermedia, F.nucelatum and S.mutans. Results: P.gingivalis and P.intermedia were sensitive to C.officinalis, while F.nucleatum and A.actinomycetemcomitans exhibited reduced sensitivity. C.officinalis was highly effective against S.mutans (3.12 µg/mL, its inhibitory concentration close to that of chlorhexidine digluconate 0.2% (6.25 µg/mL. C.officinalis took longer than chlorhexidine digluconate 0.2% to exhibit lethality against all the organisms that it inhibited. Chlorhexidine exhibited immediate lethality (zero minute against S.mutans while it inhibited the other microbes at 5 minutes. A.actinomycetemcomitans and P.intermedia were inhibited by C.officinalis at 30 minutes, S.mutans at 10 minutes. F.nucleatum was inhibited by C.officinalis at 2 hours and no inhibition of P.gingivalis by C.officinalis was observed. Conclusion: C.officinalis showed antimicrobial efficacy against most organisms tested, yet its efficacy was not on par with chlorhexidine digluconate 0.2%. However, its

  16. Antimutagenicity of some flowers grown in Thailand.

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    Wongwattanasathien, O; Kangsadalampai, K; Tongyonk, L

    2010-04-01

    The mutagenicity of dichloromethane, methanol and water extracts of Antigonon leptopus Hook. & Arn., Curcuma sessilis Gage, Hibiscus rosa-sinensis Linn., Ixora coccinea Linn., Millingtonia hortensis Linn., Nelumbo nucifera Gaertn., Plumeria obtusa Linn., Punica granatum Linn., Rhinacanthus nasutus ((Linn.) Kurz.) and Syzygium malaccense ((Linn.) Merr.& Perry) before and after nitrite treatment was firstly investigated in the Ames test. Their antimutagenicity against the product of the reaction mixture of 1-aminopyrene nitrite model in the absence of metabolic activation on Salmonella typhimurium TA 98 and TA 100 was evaluated. The results showed that none of the samples was mutagenic. Most nitrite-treated samples but dichloromethane extracts of Hibiscus rosa-sinensis, Plumeria obtusa, Syzygium malaccense, methanol extract of Syzygium malaccense and water extract of Hibiscus rosa-sinensis were mutagenic. The nitrite treated methanol extract of Nelumbo nucifera exhibited the highest mutagenicity on both strains. All dichloromethane extracts of flowers decreased the mutagenicity induced by the product of 1-aminopyrene nitrite model on both tester strains. Methanol extract of Curcuma sessilis and Punica granatum (15 mg/plate) showed the highest antimutagenic activity in TA 98 and TA 100, respectively. The protective effects of these flower extracts might be due to the presence of antimutagenic components that were supposed to be flavonoids. 2010 Elsevier Ltd. All rights reserved.

  17. Medicinal flowers. XXX. Eight new glycosides, everlastosides F-M, from the flowers of Helichrysum arenarium.

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    Morikawa, Toshio; Wang, Li-Bo; Ninomiya, Kiyofumi; Nakamura, Seikou; Matsuda, Hisashi; Muraoka, Osamu; Wu, Li-Jun; Yoshikawa, Masayuki

    2009-08-01

    Eight new glycosides, everlastosides F (1), G (2), H (3), I (4), J (5), K (6), L (7), and M (8), were isolated from the methanolic extract of the flowers of Helichrysum arenarium. Their structures were elucidated on the basis of chemical and physicochemical evidence.