WorldWideScience

Sample records for flight test identification

  1. Design of a quadrotor flight test stand for system identification

    CSIR Research Space (South Africa)

    Beharie, MM

    2015-01-01

    Full Text Available This paper presents the design, development and construction of a flight test stand for a quadrotor UAV. As opposed to alternate forms of UAV, the power plant in the case of the quadrotor serves a dual purpose of control and propulsion. Since...

  2. Flight research and testing

    Science.gov (United States)

    Putnam, Terrill W.; Ayers, Theodore G.

    1989-01-01

    Flight research and testing form a critical link in the aeronautic research and development chain. Brilliant concepts, elegant theories, and even sophisticated ground tests of flight vehicles are not sufficient to prove beyond a doubt that an unproven aeronautical concept will actually perform as predicted. Flight research and testing provide the ultimate proof that an idea or concept performs as expected. Ever since the Wright brothers, flight research and testing were the crucible in which aeronautical concepts were advanced and proven to the point that engineers and companies are willing to stake their future to produce and design aircraft. This is still true today, as shown by the development of the experimental X-30 aerospace plane. The Dryden Flight Research Center (Ames-Dryden) continues to be involved in a number of flight research programs that require understanding and characterization of the total airplane in all the aeronautical disciplines, for example the X-29. Other programs such as the F-14 variable-sweep transition flight experiment have focused on a single concept or discipline. Ames-Dryden also continues to conduct flight and ground based experiments to improve and expand the ability to test and evaluate advanced aeronautical concepts. A review of significant aeronautical flight research programs and experiments is presented to illustrate both the progress being made and the challenges to come.

  3. Identification of a Cessna Citation II Model Based on Flight Test Data

    NARCIS (Netherlands)

    de Visser, C.C.; Pool, D.M.

    2017-01-01

    As a result of new aviation legislation, from 2019 on all air-carrier pilots are obliged to go through flight simulator-based stall recovery training. For this reason the Control and Simulation division at Delft University of Technology has set up a task force to develop a new methodology for

  4. ALOFT Flight Test Report

    Science.gov (United States)

    1977-10-01

    wmmmmmmmmmmmm i ifmu.immM\\]i\\ ßinimm^mmmmviwmmiwui »vimtm twfjmmmmmmi c-f—rmSmn NWC TP 5954 ALOFT Flight Test Report by James D. Ross anrJ I.. M...responsible i"- u conducting the ALOFT Flight Test Program and made contributions to this report: J. Basden , R. ".estbrook, L. Thompson, J. Willians...REPORT DOCUMENTATION PAGE READ INSTRUCTIONS BEFORE COMPLETING FORM 7. AUTMORC«; <oss James D./Xo L. M.y&ohnson IZATION NAME AND ADDRESS Naval

  5. Flight Testing and Real-Time System Identification Analysis of a UH-60A Black Hawk Helicopter with an Instrumented External Sling Load

    Science.gov (United States)

    McCoy, Allen H.

    1998-01-01

    -real time system identification was also demonstrated during the flight test program.

  6. System Identification for Integrated Aircraft Development and Flight Testing (l’Identification des systemes pour le developpement integre des aeronefs et les essais en vol)

    Science.gov (United States)

    1999-03-01

    aerodynamics to affect load motions. The effects include a load trail angle in proportion to the drag specific force, and modification of the load pendulum...equations algorithm for flight data filtering architeture . and data consistency checking; and SCIDNT 8, an output architecture. error identification...accelerations at the seven sensor locations, identified system is proportional to the number When system identification is performed, as of flexible modes

  7. Weather and Flight Testing

    Science.gov (United States)

    Wiley, Scott

    2007-01-01

    This viewgraph document reviews some of the weather hazards involved with flight testing. Some of the hazards reviewed are: turbulence, icing, thunderstorms and winds and windshear. Maps, pictures, satellite pictures of the meteorological phenomena and graphs are included. Also included are pictures of damaged aircraft.

  8. F-18 High Alpha Research Vehicle (HARV) parameter identification flight test maneuvers for optimal input design validation and lateral control effectiveness

    Science.gov (United States)

    Morelli, Eugene A.

    1995-01-01

    Flight test maneuvers are specified for the F-18 High Alpha Research Vehicle (HARV). The maneuvers were designed for open loop parameter identification purposes, specifically for optimal input design validation at 5 degrees angle of attack, identification of individual strake effectiveness at 40 and 50 degrees angle of attack, and study of lateral dynamics and lateral control effectiveness at 40 and 50 degrees angle of attack. Each maneuver is to be realized by applying square wave inputs to specific control effectors using the On-Board Excitation System (OBES). Maneuver descriptions and complete specifications of the time/amplitude points define each input are included, along with plots of the input time histories.

  9. Flight Test of an Intelligent Flight-Control System

    Science.gov (United States)

    Davidson, Ron; Bosworth, John T.; Jacobson, Steven R.; Thomson, Michael Pl; Jorgensen, Charles C.

    2003-01-01

    inputs with the outputs provided to instrumentation only. The IFCS was not used to control the airplane. In another stage of the flight test, the Phase I pre-trained neural network was integrated into a Phase III version of the flight control system. The Phase I pretrained neural network provided realtime stability and control derivatives to a Phase III controller that was based on a stochastic optimal feedforward and feedback technique (SOFFT). This combined Phase I/III system was operated together with the research flight-control system (RFCS) of the F-15 ACTIVE during the flight test. The RFCS enables the pilot to switch quickly from the experimental- research flight mode back to the safe conventional mode. These initial IFCS ACP flight tests were completed in April 1999. The Phase I/III flight test milestone was to demonstrate, across a range of subsonic and supersonic flight conditions, that the pre-trained neural network could be used to supply real-time aerodynamic stability and control derivatives to the closed-loop optimal SOFFT flight controller. Additional objectives attained in the flight test included (1) flight qualification of a neural-network-based control system; (2) the use of a combined neural-network/closed-loop optimal flight-control system to obtain level-one handling qualities; and (3) demonstration, through variation of control gains, that different handling qualities can be achieved by setting new target parameters. In addition, data for the Phase-II (on-line-learning) neural network were collected, during the use of stacked-frequency- sweep excitation, for post-flight analysis. Initial analysis of these data showed the potential for future flight tests that will incorporate the real-time identification and on-line learning aspects of the IFCS.

  10. Selected Flight Test Results for Online Learning Neural Network-Based Flight Control System

    Science.gov (United States)

    Williams-Hayes, Peggy S.

    2004-01-01

    The NASA F-15 Intelligent Flight Control System project team developed a series of flight control concepts designed to demonstrate neural network-based adaptive controller benefits, with the objective to develop and flight-test control systems using neural network technology to optimize aircraft performance under nominal conditions and stabilize the aircraft under failure conditions. This report presents flight-test results for an adaptive controller using stability and control derivative values from an online learning neural network. A dynamic cell structure neural network is used in conjunction with a real-time parameter identification algorithm to estimate aerodynamic stability and control derivative increments to baseline aerodynamic derivatives in flight. This open-loop flight test set was performed in preparation for a future phase in which the learning neural network and parameter identification algorithm output would provide the flight controller with aerodynamic stability and control derivative updates in near real time. Two flight maneuvers are analyzed - pitch frequency sweep and automated flight-test maneuver designed to optimally excite the parameter identification algorithm in all axes. Frequency responses generated from flight data are compared to those obtained from nonlinear simulation runs. Flight data examination shows that addition of flight-identified aerodynamic derivative increments into the simulation improved aircraft pitch handling qualities.

  11. Aviation Flight Test

    Data.gov (United States)

    Federal Laboratory Consortium — Redstone Test Center provides an expert workforce and technologically advanced test equipment to conduct the rigorous testing necessary for U.S. Army acquisition and...

  12. Integrated Test and Evaluation Flight Test 3 Flight Test Plan

    Science.gov (United States)

    Marston, Michael Lawrence

    2015-01-01

    The desire and ability to fly Unmanned Aircraft Systems (UAS) in the National Airspace System (NAS) is of increasing urgency. The application of unmanned aircraft to perform national security, defense, scientific, and emergency management are driving the critical need for less restrictive access by UAS to the NAS. UAS represent a new capability that will provide a variety of services in the government (public) and commercial (civil) aviation sectors. The growth of this potential industry has not yet been realized due to the lack of a common understanding of what is required to safely operate UAS in the NAS. NASA's UAS Integration into the NAS Project is conducting research in the areas of Separation Assurance/Sense and Avoid Interoperability, Human Systems Integration (HSI), and Communication to support reducing the barriers of UAS access to the NAS. This research is broken into two research themes namely, UAS Integration and Test Infrastructure. UAS Integration focuses on airspace integration procedures and performance standards to enable UAS integration in the air transportation system, covering Sense and Avoid (SAA) performance standards, command and control performance standards, and human systems integration. The focus of Test Infrastructure is to enable development and validation of airspace integration procedures and performance standards, including the integrated test and evaluation. In support of the integrated test and evaluation efforts, the Project will develop an adaptable, scalable, and schedulable relevant test environment capable of evaluating concepts and technologies for unmanned aircraft systems to safely operate in the NAS. To accomplish this task, the Project will conduct a series of Human-in-the-Loop and Flight Test activities that integrate key concepts, technologies and/or procedures in a relevant air traffic environment. Each of the integrated events will build on the technical achievements, fidelity and complexity of the previous tests and

  13. Supersonic Retropropulsion Flight Test Concepts

    Science.gov (United States)

    Post, Ethan A.; Dupzyk, Ian C.; Korzun, Ashley M.; Dyakonov, Artem A.; Tanimoto, Rebekah L.; Edquist, Karl T.

    2011-01-01

    NASA's Exploration Technology Development and Demonstration Program has proposed plans for a series of three sub-scale flight tests at Earth for supersonic retropropulsion, a candidate decelerator technology for future, high-mass Mars missions. The first flight test in this series is intended to be a proof-of-concept test, demonstrating successful initiation and operation of supersonic retropropulsion at conditions that replicate the relevant physics of the aerodynamic-propulsive interactions expected in flight. Five sub-scale flight test article concepts, each designed for launch on sounding rockets, have been developed in consideration of this proof-of-concept flight test. Commercial, off-the-shelf components are utilized as much as possible in each concept. The design merits of the concepts are compared along with their predicted performance for a baseline trajectory. The results of a packaging study and performance-based trade studies indicate that a sounding rocket is a viable launch platform for this proof-of-concept test of supersonic retropropulsion.

  14. Recursive subspace identification for in flight modal analysis of airplanes

    OpenAIRE

    De Cock , Katrien; Mercère , Guillaume; De Moor , Bart

    2006-01-01

    International audience; In this paper recursive subspace identification algorithms are applied to track the modal parameters of airplanes on-line during test flights. The ability to track changes in the damping ratios and the influence of the forgetting factor are studied through simulations.

  15. Flight Test Techniques

    Science.gov (United States)

    1989-01-01

    the airframe for col1A 300 jauges pour un essal d𔄀preuve a ground "proof test" with a 0.8 charge, au sol A charge 0,8 recalant ce modile ; to update...the model. 200 among these 200 de ces jauges ont 6t6 cibl~es bonnes gauges had been wired to be "airworthy", de vol mats le domaine de vol a 4t6 but

  16. Initial Flight Test of the Production Support Flight Control Computers at NASA Dryden Flight Research Center

    Science.gov (United States)

    Carter, John; Stephenson, Mark

    1999-01-01

    The NASA Dryden Flight Research Center has completed the initial flight test of a modified set of F/A-18 flight control computers that gives the aircraft a research control law capability. The production support flight control computers (PSFCC) provide an increased capability for flight research in the control law, handling qualities, and flight systems areas. The PSFCC feature a research flight control processor that is "piggybacked" onto the baseline F/A-18 flight control system. This research processor allows for pilot selection of research control law operation in flight. To validate flight operation, a replication of a standard F/A-18 control law was programmed into the research processor and flight-tested over a limited envelope. This paper provides a brief description of the system, summarizes the initial flight test of the PSFCC, and describes future experiments for the PSFCC.

  17. Flight test trajectory control analysis

    Science.gov (United States)

    Walker, R.; Gupta, N.

    1983-01-01

    Recent extensions to optimal control theory applied to meaningful linear models with sufficiently flexible software tools provide powerful techniques for designing flight test trajectory controllers (FTTCs). This report describes the principal steps for systematic development of flight trajectory controllers, which can be summarized as planning, modeling, designing, and validating a trajectory controller. The techniques have been kept as general as possible and should apply to a wide range of problems where quantities must be computed and displayed to a pilot to improve pilot effectiveness and to reduce workload and fatigue. To illustrate the approach, a detailed trajectory guidance law is developed and demonstrated for the F-15 aircraft flying the zoom-and-pushover maneuver.

  18. Remotely Piloted Vehicles for Experimental Flight Control Testing

    Science.gov (United States)

    Motter, Mark A.; High, James W.

    2009-01-01

    A successful flight test and training campaign of the NASA Flying Controls Testbed was conducted at Naval Outlying Field, Webster Field, MD during 2008. Both the prop and jet-powered versions of the subscale, remotely piloted testbeds were used to test representative experimental flight controllers. These testbeds were developed by the Subsonic Fixed Wing Project s emphasis on new flight test techniques. The Subsonic Fixed Wing Project is under the Fundamental Aeronautics Program of NASA's Aeronautics Research Mission Directorate (ARMD). The purpose of these testbeds is to quickly and inexpensively evaluate advanced concepts and experimental flight controls, with applications to adaptive control, system identification, novel control effectors, correlation of subscale flight tests with wind tunnel results, and autonomous operations. Flight tests and operator training were conducted during four separate series of tests during April, May, June and August 2008. Experimental controllers were engaged and disengaged during fully autonomous flight in the designated test area. Flaps and landing gear were deployed by commands from the ground control station as unanticipated disturbances. The flight tests were performed NASA personnel with support from the Maritime Unmanned Development and Operations (MUDO) team of the Naval Air Warfare Center, Aircraft Division

  19. System Identification of Flight Mechanical Characteristics

    OpenAIRE

    Larsson, Roger

    2013-01-01

    With the demand for more advanced fighter aircraft, relying on relaxed stability or even unstable flight mechanical characteristics to gain flight performance, more focus has been put on model-based system engineering to help with the design work. The flight control system design is one important part that relies on this modeling. Therefore it has become more important to develop flight mechanical models that are highly accurate in the whole flight envelop. For today’s newly developed fighter...

  20. The Orion Exploration Flight Test Post Flight Solid Particle Flight Environment Inspection and Analysis

    Science.gov (United States)

    Miller, Joshua E.

    2016-01-01

    Orbital debris in the millimeter size range can pose a hazard to current and planned spacecraft due to the high relative impact speeds in Earth orbit. Fortunately, orbital debris has a relatively short life at lower altitudes due to atmospheric effects; however, at higher altitudes orbital debris can survive much longer and has resulted in a band of high flux around 700 to 1,500 km above the surface of the Earth. While large orbital debris objects are tracked via ground based observation, little information can be gathered about small particles except by returned surfaces, which until the Orion Exploration Flight Test number one (EFT-1), has only been possible for lower altitudes (400 to 500 km). The EFT-1 crew module backshell, which used a porous, ceramic tile system with surface coatings, has been inspected post-flight for potential micrometeoroid and orbital debris (MMOD) damage. This paper describes the pre- and post-flight activities of inspection, identification and analysis of six candidate MMOD impact craters from the EFT-1 mission.

  1. Rotationally Adaptive Flight Test Surface

    Science.gov (United States)

    Barrett, Ron

    1999-01-01

    Research on a new design of flutter exciter vane using adaptive materials was conducted. This novel design is based on all-moving aerodynamic surface technology and consists of a structurally stiff main spar, a series of piezoelectric actuator elements and an aerodynamic shell which is pivoted around the main spar. The work was built upon the current missile-type all-moving surface designs and change them so they are better suited for flutter excitation through the transonic flight regime. The first portion of research will be centered on aerodynamic and structural modeling of the system. USAF DatCom and vortex lattice codes was used to capture the fundamental aerodynamics of the vane. Finite element codes and laminated plate theory and virtual work analyses will be used to structurally model the aerodynamic vane and wing tip. Following the basic modeling, a flutter test vane was designed. Each component within the structure was designed to meet the design loads. After the design loads are met, then the deflections will be maximized and the internal structure will be laid out. In addition to the structure, a basic electrical control network will be designed which will be capable of driving a scaled exciter vane. The third and final stage of main investigation involved the fabrication of a 1/4 scale vane. This scaled vane was used to verify kinematics and structural mechanics theories on all-moving actuation. Following assembly, a series of bench tests was conducted to determine frequency response, electrical characteristics, mechanical and kinematic properties. Test results indicate peak-to-peak deflections of 1.1 deg with a corner frequency of just over 130 Hz.

  2. Flight Test Maneuvers for Efficient Aerodynamic Modeling

    Science.gov (United States)

    Morelli, Eugene A.

    2011-01-01

    Novel flight test maneuvers for efficient aerodynamic modeling were developed and demonstrated in flight. Orthogonal optimized multi-sine inputs were applied to aircraft control surfaces to excite aircraft dynamic response in all six degrees of freedom simultaneously while keeping the aircraft close to chosen reference flight conditions. Each maneuver was designed for a specific modeling task that cannot be adequately or efficiently accomplished using conventional flight test maneuvers. All of the new maneuvers were first described and explained, then demonstrated on a subscale jet transport aircraft in flight. Real-time and post-flight modeling results obtained using equation-error parameter estimation in the frequency domain were used to show the effectiveness and efficiency of the new maneuvers, as well as the quality of the aerodynamic models that can be identified from the resultant flight data.

  3. Flight Test Approach to Adaptive Control Research

    Science.gov (United States)

    Pavlock, Kate Maureen; Less, James L.; Larson, David Nils

    2011-01-01

    The National Aeronautics and Space Administration s Dryden Flight Research Center completed flight testing of adaptive controls research on a full-scale F-18 testbed. The validation of adaptive controls has the potential to enhance safety in the presence of adverse conditions such as structural damage or control surface failures. This paper describes the research interface architecture, risk mitigations, flight test approach and lessons learned of adaptive controls research.

  4. FT 3 Flight Test Cards for Export

    Science.gov (United States)

    Marston, Michael L.

    2015-01-01

    These flight test cards will be made available to stakeholders who participated in FT3. NASA entered into the relationship with our stakeholders, including the FAA, to develop requirements that will lead to routine flights of unmanned aircraft systems flying in the national airspace system.

  5. Application of identification techniques to remote manipulator system flight data

    Science.gov (United States)

    Shepard, G. D.; Lepanto, J. A.; Metzinger, R. W.; Fogel, E.

    1983-01-01

    This paper addresses the application of identification techniques to flight data from the Space Shuttle Remote Manipulator System (RMS). A description of the remote manipulator, including structural and control system characteristics, sensors, and actuators is given. A brief overview of system identification procedures is presented, and the practical aspects of implementing system identification algorithms are discussed. In particular, the problems posed by desampling rate, numerical error, and system nonlinearities are considered. Simulation predictions of damping, frequency, and system order are compared with values identified from flight data to support an evaluation of RMS structural and control system models. Finally, conclusions are drawn regarding the application of identification techniques to flight data obtained from a flexible space structure.

  6. Ares I-X Flight Test Philosophy

    Science.gov (United States)

    Davis, S. R.; Tuma, M. L.; Heitzman, K.

    2007-01-01

    In response to the Vision for Space Exploration, the National Aeronautics and Space Administration (NASA) has defined a new space exploration architecture to return humans to the Moon and prepare for human exploration of Mars. One of the first new developments will be the Ares I Crew Launch Vehicle (CLV), which will carry the Orion Crew Exploration Vehicle (CEV), into Low Earth Orbit (LEO) to support International Space Station (ISS) missions and, later, support lunar missions. As part of Ares I development, NASA will perform a series of Ares I flight tests. The tests will provide data that will inform the engineering and design process and verify the flight hardware and software. The data gained from the flight tests will be used to certify the new Ares/Orion vehicle for human space flight. The primary objectives of this first flight test (Ares I-X) are the following: Demonstrate control of a dynamically similar integrated Ares CLV/Orion CEV using Ares CLV ascent control algorithms; Perform an in-flight separation/staging event between an Ares I-similar First Stage and a representative Upper Stage; Demonstrate assembly and recovery of a new Ares CLV-like First Stage element at Kennedy Space Center (KSC); Demonstrate First Stage separation sequencing, and quantify First Stage atmospheric entry dynamics and parachute performance; and Characterize the magnitude of the integrated vehicle roll torque throughout the First Stage (powered) flight. This paper will provide an overview of the Ares I-X flight test process and details of the individual flight tests.

  7. Laser Obstacle Detection System Flight Testing

    National Research Council Canada - National Science Library

    Davis, Timothy

    2003-01-01

    ...). The Aviation Applied Technology Directorate (AATD) was contracted to mount the HELLAS sensor on the nose of a UH-60L Blackhawk helicopter and to conduct flight tests to evaluate the HELLAS obstacle detection sensor...

  8. Writing executable assertions to test flight software

    Science.gov (United States)

    Mahmood, A.; Andrews, D. M.; Mccluskey, E. J.

    1984-01-01

    An executable assertion is a logical statement about the variables or a block of code. If there is no error during execution, the assertion statement results in a true value. Executable assertions can be used for dynamic testing of software. They can be employed for validation during the design phase, and exception and error detection during the operation phase. The present investigation is concerned with the problem of writing executable assertions, taking into account the use of assertions for testing flight software. They can be employed for validation during the design phase, and for exception handling and error detection during the operation phase The digital flight control system and the flight control software are discussed. The considered system provides autopilot and flight director modes of operation for automatic and manual control of the aircraft during all phases of flight. Attention is given to techniques for writing and using assertions to test flight software, an experimental setup to test flight software, and language features to support efficient use of assertions.

  9. Astronaut Gordon Cooper during flight tests

    Science.gov (United States)

    1963-01-01

    Astronaut L. Gordon Cooper, prime pilot for the Mercury-Atlas 9 mission, relaxes while waiting for weight and balance tests to begin (03974); Cooper prior to entering the Mercury Spacecraft for a series of simulated flight tests. During these tests NASA doctors, engineers and technicians monitor Cooper's performance (03975); Cooper undergoing suit pressurization tests (03976).

  10. Direct Identification and Antimicrobial Susceptibility Testing of Bacteria From Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and the Vitek 2 System.

    Science.gov (United States)

    Jo, Sung Jin; Park, Kang Gyun; Han, Kyungja; Park, Dong Jin; Park, Yeon-Joon

    2016-03-01

    We evaluated the reliability and accuracy of the combined use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial identification and Vitek 2 antimicrobial susceptibility testing (AST) for bacteria from positive blood culture bottles. Direct identification and AST were performed in parallel to the standard methods in monomicrobial positive blood culture bottles. In total, 254 isolates grown on aerobic and/or anaerobic bottles were identified with MALDI-TOF Vitek MS (bioMérieux, France), and 1,978 microorganism/antimicrobial agent combinations were assessed. For isolates from anaerobic bottles, an aliquot of the culture broth was centrifuged, washed, and filtered through a nylon mesh. For isolates from aerobic/pediatric bottles, a lysis step using 9.26% ammonium chloride solution and 2% saponin solution was included. The overall correct identification rate was 81.8% (208/254) and that for gram-positive/gram-negative isolates was 73.9%/92.6%, respectively, and it was 81.8%, 87.6%, and 57.9% for isolates from aerobic, anaerobic, and pediatric bottles, respectively. Identification was not possible in 45 cases, and most of these isolates were streptococci (N=14) and coagulase-negative staphylococci (N=11). Misidentification occurred only in one case. Compared with standard methods, direct AST showed 97.9% (1,936/1,978) agreement with very major error of 0.25%, major error of 0.05%, and minor error of 1.8%. This simple and cost-effective sample preparation method gives reliable results for the direct identification and AST of bacteria. For the identification of streptococci and coagulase-negative staphylococci, the method should be further improved.

  11. Orion Exploration Flight Test Post-Flight Inspection and Analysis

    Science.gov (United States)

    Miller, J. E.; Berger, E. L.; Bohl, W. E.; Christiansen, E. L.; Davis, B. A.; Deighton, K. D.; Enriquez, P. A.; Garcia, M. A.; Hyde, J. L.; Oliveras, O. M.

    2017-01-01

    The principal mechanism for developing orbital debris environment models, is to make observations of larger pieces of debris in the range of several centimeters and greater using radar and optical techniques. For particles that are smaller than this threshold, breakup and migration models of particles to returned surfaces in lower orbit are relied upon to quantify the flux. This reliance on models to derive spatial densities of particles that are of critical importance to spacecraft make the unique nature of the EFT-1's return surface a valuable metric. To this end detailed post-flight inspections have been performed of the returned EFT-1 backshell, and the inspections identified six candidate impact sites that were not present during the pre-flight inspections. This paper describes the post-flight analysis efforts to characterize the EFT-1 mission craters. This effort included ground based testing to understand small particle impact craters in the thermal protection material, the pre- and post-flight inspection, the crater analysis using optical, X-ray computed tomography (CT) and scanning electron microscope (SEM) techniques, and numerical simulations.

  12. Development of a flight software testing methodology

    Science.gov (United States)

    Mccluskey, E. J.; Andrews, D. M.

    1985-01-01

    The research to develop a testing methodology for flight software is described. An experiment was conducted in using assertions to dynamically test digital flight control software. The experiment showed that 87% of typical errors introduced into the program would be detected by assertions. Detailed analysis of the test data showed that the number of assertions needed to detect those errors could be reduced to a minimal set. The analysis also revealed that the most effective assertions tested program parameters that provided greater indirect (collateral) testing of other parameters. In addition, a prototype watchdog task system was built to evaluate the effectiveness of executing assertions in parallel by using the multitasking features of Ada.

  13. Fused Reality for Enhanced Flight Test Capabilities

    Science.gov (United States)

    Bachelder, Ed; Klyde, David

    2011-01-01

    The feasibility of using Fused Reality-based simulation technology to enhance flight test capabilities has been investigated. In terms of relevancy to piloted evaluation, there remains no substitute for actual flight tests, even when considering the fidelity and effectiveness of modern ground-based simulators. In addition to real-world cueing (vestibular, visual, aural, environmental, etc.), flight tests provide subtle but key intangibles that cannot be duplicated in a ground-based simulator. There is, however, a cost to be paid for the benefits of flight in terms of budget, mission complexity, and safety, including the need for ground and control-room personnel, additional aircraft, etc. A Fused Reality(tm) (FR) Flight system was developed that allows a virtual environment to be integrated with the test aircraft so that tasks such as aerial refueling, formation flying, or approach and landing can be accomplished without additional aircraft resources or the risk of operating in close proximity to the ground or other aircraft. Furthermore, the dynamic motions of the simulated objects can be directly correlated with the responses of the test aircraft. The FR Flight system will allow real-time observation of, and manual interaction with, the cockpit environment that serves as a frame for the virtual out-the-window scene.

  14. Free Flight Rotorcraft Flight Test Vehicle Technology Development

    Science.gov (United States)

    Hodges, W. Todd; Walker, Gregory W.

    1994-01-01

    A rotary wing, unmanned air vehicle (UAV) is being developed as a research tool at the NASA Langley Research Center by the U.S. Army and NASA. This development program is intended to provide the rotorcraft research community an intermediate step between rotorcraft wind tunnel testing and full scale manned flight testing. The technologies under development for this vehicle are: adaptive electronic flight control systems incorporating artificial intelligence (AI) techniques, small-light weight sophisticated sensors, advanced telepresence-telerobotics systems and rotary wing UAV operational procedures. This paper briefly describes the system's requirements and the techniques used to integrate the various technologies to meet these requirements. The paper also discusses the status of the development effort. In addition to the original aeromechanics research mission, the technology development effort has generated a great deal of interest in the UAV community for related spin-off applications, as briefly described at the end of the paper. In some cases the technologies under development in the free flight program are critical to the ability to perform some applications.

  15. Fuel Subsystems Flight Test Handbook

    Science.gov (United States)

    1981-12-01

    detailed, accessible .-ltand complete test records for his own protection and for the benefit of his successor in case of promotion, transfer or...and pilot display of fuel quantity, low level warning and a " Bingo " fudl warning. 3.0 TEST OBJECT1VES: ACTION Orriclc. On POUTION•’PHONWE [ DATE PCR...TIS No. 46, paragraph 3.11 3.6 To demonstrate that the low level and bingo warning system are consistent L •and meet the requirements of paragraph

  16. The development of a Flight Test Engineer's Workstation for the Automated Flight Test Management System

    Science.gov (United States)

    Tartt, David M.; Hewett, Marle D.; Duke, Eugene L.; Cooper, James A.; Brumbaugh, Randal W.

    1989-01-01

    The Automated Flight Test Management System (ATMS) is being developed as part of the NASA Aircraft Automation Program. This program focuses on the application of interdisciplinary state-of-the-art technology in artificial intelligence, control theory, and systems methodology to problems of operating and flight testing high-performance aircraft. The development of a Flight Test Engineer's Workstation (FTEWS) is presented, with a detailed description of the system, technical details, and future planned developments. The goal of the FTEWS is to provide flight test engineers and project officers with an automated computer environment for planning, scheduling, and performing flight test programs. The FTEWS system is an outgrowth of the development of ATMS and is an implementation of a component of ATMS on SUN workstations.

  17. Integrated Test and Evaluation (ITE) Flight Test Series 4

    Science.gov (United States)

    Marston, Michael

    2016-01-01

    The integrated Flight Test 4 (FT4) will gather data for the UAS researchers Sense and Avoid systems (referred to as Detect and Avoid in the RTCA SC 228 ToR) algorithms and pilot displays for candidate UAS systems in a relevant environment. The technical goals of FT4 are to: 1) perform end-to-end traffic encounter test of pilot guidance generated by DAA algorithms; 2) collect data to inform the initial Minimum Operational Performance Standards (MOPS) for Detect and Avoid systems. FT4 objectives and test infrastructure builds from previous UAS project simulations and flight tests. NASA Ames (ARC), NASA Armstrong (AFRC), and NASA Langley (LaRC) Research Centers will share responsibility for conducting the tests, each providing a test lab and critical functionality. UAS-NAS project support and participation on the 2014 flight test of ACAS Xu and DAA Self Separation (SS) significantly contributed to building up infrastructure and procedures for FT3 as well. The DAA Scripted flight test (FT4) will be conducted out of NASA Armstrong over an eight-week period beginning in April 2016.

  18. Free Flight Ground Testing of ADEPT in Advance of the Sounding Rocket One Flight Experiment

    Science.gov (United States)

    Smith, B. P.; Dutta, S.

    2017-01-01

    The Adaptable Deployable Entry and Placement Technology (ADEPT) project will be conducting the first flight test of ADEPT, titled Sounding Rocket One (SR-1), in just two months. The need for this flight test stems from the fact that ADEPT's supersonic dynamic stability has not yet been characterized. The SR-1 flight test will provide critical data describing the flight mechanics of ADEPT in ballistic flight. These data will feed decision making on future ADEPT mission designs. This presentation will describe the SR-1 scientific data products, possible flight test outcomes, and the implications of those outcomes on future ADEPT development. In addition, this presentation will describe free-flight ground testing performed in advance of the flight test. A subsonic flight dynamics test conducted at the Vertical Spin Tunnel located at NASA Langley Research Center provided subsonic flight dynamics data at high and low altitudes for multiple center of mass (CoM) locations. A ballistic range test at the Hypervelocity Free Flight Aerodynamics Facility (HFFAF) located at NASA Ames Research Center provided supersonic flight dynamics data at low supersonic Mach numbers. Execution and outcomes of these tests will be discussed. Finally, a hypothesized trajectory estimate for the SR-1 flight will be presented.

  19. Urine testing for designing steroids by liquid chromatography and androgen bioaasay detection and electrospray quadrupole time-of-flight mass spectrometry identification

    NARCIS (Netherlands)

    Nielen, M.W.F.; Bovee, T.F.H.; Engelen, M.C.; Rutgers, P.; Hamers, A.R.M.; Rhijn, van J.A.; Hoogenboom, L.A.P.

    2006-01-01

    New anabolic steroids show up occasionally in sports doping and in veterinary control. The discovery of these designer steroids is facilitated by findings of illicit preparations, thus allowing bioactivity testing, structure elucidation using NMR and mass spectrometry, and final incorporation in

  20. Implementation and flight tests for the Digital Integrated Automatic Landing System (DIALS). Part 1: Flight software equations, flight test description and selected flight test data

    Science.gov (United States)

    Hueschen, R. M.

    1986-01-01

    Five flight tests of the Digital Automated Landing System (DIALS) were conducted on the Advanced Transport Operating Systems (ATOPS) Transportation Research Vehicle (TSRV) -- a modified Boeing 737 aircraft for advanced controls and displays research. These flight tests were conducted at NASA's Wallops Flight Center using the microwave landing system (MLS) installation on runway 22. This report describes the flight software equations of the DIALS which was designed using modern control theory direct-digital design methods and employed a constant gain Kalman filter. Selected flight test performance data is presented for localizer (runway centerline) capture and track at various intercept angles, for glideslope capture and track of 3, 4.5, and 5 degree glideslopes, for the decrab maneuver, and for the flare maneuver. Data is also presented to illustrate the system performance in the presence of cross, gust, and shear winds. The mean and standard deviation of the peak position errors for localizer capture were, respectively, 24 feet and 26 feet. For mild wind conditions, glideslope and localizer tracking position errors did not exceed, respectively, 5 and 20 feet. For gusty wind conditions (8 to 10 knots), these errors were, respectively, 10 and 30 feet. Ten hands off automatic lands were performed. The standard deviation of the touchdown position and velocity errors from the mean values were, respectively, 244 feet and 0.7 feet/sec.

  1. 14 CFR 21.37 - Flight test pilot.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Flight test pilot. 21.37 Section 21.37... PROCEDURES FOR PRODUCTS AND PARTS Type Certificates § 21.37 Flight test pilot. Each applicant for a normal... holding an appropriate pilot certificate to make the flight tests required by this part. [Doc. No. 5085...

  2. Automation of Flight Software Regression Testing

    Science.gov (United States)

    Tashakkor, Scott B.

    2016-01-01

    NASA is developing the Space Launch System (SLS) to be a heavy lift launch vehicle supporting human and scientific exploration beyond earth orbit. SLS will have a common core stage, an upper stage, and different permutations of boosters and fairings to perform various crewed or cargo missions. Marshall Space Flight Center (MSFC) is writing the Flight Software (FSW) that will operate the SLS launch vehicle. The FSW is developed in an incremental manner based on "Agile" software techniques. As the FSW is incrementally developed, testing the functionality of the code needs to be performed continually to ensure that the integrity of the software is maintained. Manually testing the functionality on an ever-growing set of requirements and features is not an efficient solution and therefore needs to be done automatically to ensure testing is comprehensive. To support test automation, a framework for a regression test harness has been developed and used on SLS FSW. The test harness provides a modular design approach that can compile or read in the required information specified by the developer of the test. The modularity provides independence between groups of tests and the ability to add and remove tests without disturbing others. This provides the SLS FSW team a time saving feature that is essential to meeting SLS Program technical and programmatic requirements. During development of SLS FSW, this technique has proved to be a useful tool to ensure all requirements have been tested, and that desired functionality is maintained, as changes occur. It also provides a mechanism for developers to check functionality of the code that they have developed. With this system, automation of regression testing is accomplished through a scheduling tool and/or commit hooks. Key advantages of this test harness capability includes execution support for multiple independent test cases, the ability for developers to specify precisely what they are testing and how, the ability to add

  3. Integration of Online Parameter Identification and Neural Network for In-Flight Adaptive Control

    Science.gov (United States)

    Hageman, Jacob J.; Smith, Mark S.; Stachowiak, Susan

    2003-01-01

    An indirect adaptive system has been constructed for robust control of an aircraft with uncertain aerodynamic characteristics. This system consists of a multilayer perceptron pre-trained neural network, online stability and control derivative identification, a dynamic cell structure online learning neural network, and a model following control system based on the stochastic optimal feedforward and feedback technique. The pre-trained neural network and model following control system have been flight-tested, but the online parameter identification and online learning neural network are new additions used for in-flight adaptation of the control system model. A description of the modification and integration of these two stand-alone software packages into the complete system in preparation for initial flight tests is presented. Open-loop results using both simulation and flight data, as well as closed-loop performance of the complete system in a nonlinear, six-degree-of-freedom, flight validated simulation, are analyzed. Results show that this online learning system, in contrast to the nonlearning system, has the ability to adapt to changes in aerodynamic characteristics in a real-time, closed-loop, piloted simulation, resulting in improved flying qualities.

  4. Optimization models for flight test scheduling

    Science.gov (United States)

    Holian, Derreck

    with restriction removal is based on heuristic approaches to support the reality of flight test in both solution space and computational time. Exact methods for yielding an optimized solution will be discussed however they are not directly applicable to the flight test problem and therefore have not been included in the system.

  5. Development flight tests of JetStar LFC leading-edge flight test experiment

    Science.gov (United States)

    Fisher, David F.; Fischer, Michael C.

    1987-01-01

    The overall objective of the flight tests on the JetStar aircraft was to demonstrate the effectiveness and reliability of laminar flow control under representative flight conditions. One specific objective was to obtain laminar flow on the JetStar leading-edge test articles for the design and off-design conditions. Another specific objective was to obtain operational experience on a Laminar Flow Control (LFC) leading-edge system in a simulated airline service. This included operational experience with cleaning requirements, the effect of clogging, possible foreign object damage, erosion, and the effects of ice particle and cloud encounters. Results are summarized.

  6. UAS-NAS Flight Test Series 3: Test Environment Report

    Science.gov (United States)

    Hoang, Ty; Murphy, Jim; Otto, Neil

    2016-01-01

    The desire and ability to fly Unmanned Aircraft Systems (UAS) in the National Airspace System (NAS) is of increasing urgency. The application of unmanned aircraft to perform national security, defense, scientific, and emergency management are driving the critical need for less restrictive access by UAS to the NAS. UAS represent a new capability that will provide a variety of services in the government (public) and commercial (civil) aviation sectors. The growth of this potential industry has not yet been realized due to the lack of a common understanding of what is required to safely operate UAS in the NAS. NASA's UAS Integration in the NAS Project is conducting research in the areas of Separation Assurance/Sense and Avoid Interoperability (SSI), Human Systems Integration (HSI), and Communications (Comm), and Certification to support reducing the barriers of UAS access to the NAS. This research is broken into two research themes namely, UAS Integration and Test Infrastructure. UAS Integration focuses on airspace integration procedures and performance standards to enable UAS integration in the air transportation system, covering Detect and Avoid (DAA) performance standards, command and control performance standards, and human systems integration. The focus of Test Infrastructure is to enable development and validation of airspace integration procedures and performance standards, including integrated test and evaluation. In support of the integrated test and evaluation efforts, the Project will develop an adaptable, scalable, and schedulable relevant test environment capable of evaluating concepts and technologies for unmanned aircraft systems to safely operate in the NAS. To accomplish this task, the Project is conducting a series of human-in-the-loop (HITL) and flight test activities that integrate key concepts, technologies and/or procedures in a relevant air traffic environment. Each of the integrated events will build on the technical achievements, fidelity, and

  7. Imaging Sensor Flight and Test Equipment Software

    Science.gov (United States)

    Freestone, Kathleen; Simeone, Louis; Robertson, Byran; Frankford, Maytha; Trice, David; Wallace, Kevin; Wilkerson, DeLisa

    2007-01-01

    The Lightning Imaging Sensor (LIS) is one of the components onboard the Tropical Rainfall Measuring Mission (TRMM) satellite, and was designed to detect and locate lightning over the tropics. The LIS flight code was developed to run on a single onboard digital signal processor, and has operated the LIS instrument since 1997 when the TRMM satellite was launched. The software provides controller functions to the LIS Real-Time Event Processor (RTEP) and onboard heaters, collects the lightning event data from the RTEP, compresses and formats the data for downlink to the satellite, collects housekeeping data and formats the data for downlink to the satellite, provides command processing and interface to the spacecraft communications and data bus, and provides watchdog functions for error detection. The Special Test Equipment (STE) software was designed to operate specific test equipment used to support the LIS hardware through development, calibration, qualification, and integration with the TRMM spacecraft. The STE software provides the capability to control instrument activation, commanding (including both data formatting and user interfacing), data collection, decompression, and display and image simulation. The LIS STE code was developed for the DOS operating system in the C programming language. Because of the many unique data formats implemented by the flight instrument, the STE software was required to comprehend the same formats, and translate them for the test operator. The hardware interfaces to the LIS instrument using both commercial and custom computer boards, requiring that the STE code integrate this variety into a working system. In addition, the requirement to provide RTEP test capability dictated the need to provide simulations of background image data with short-duration lightning transients superimposed. This led to the development of unique code used to control the location, intensity, and variation above background for simulated lightning strikes

  8. Fused Reality for Enhanced Flight Test Capabilities, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — While modern ground-based flight simulators continue to improve in fidelity and effectiveness, there remains no substitute for flight test evaluations. In addition...

  9. Apollo experience report: Development flight instrumentation. [telemetry equipment for space flight test program

    Science.gov (United States)

    Farmer, N. B.

    1974-01-01

    Development flight instrumentation was delivered for 25 Apollo vehicles as Government-furnished equipment. The problems and philosophies of an activity that was concerned with supplying telemetry equipment to a space-flight test program are discussed. Equipment delivery dates, system-design details, and flight-performance information for each mission also are included.

  10. Mars Science Laboratory Flight Software Internal Testing

    Science.gov (United States)

    Jones, Justin D.; Lam, Danny

    2011-01-01

    The Mars Science Laboratory (MSL) team is sending the rover, Curiosity, to Mars, and therefore is physically and technically complex. During my stay, I have assisted the MSL Flight Software (FSW) team in implementing functional test scripts to ensure that the FSW performs to the best of its abilities. There are a large number of FSW requirements that have been written up for implementation; however I have only been assigned a few sections of these requirements. There are many stages within testing; one of the early stages is FSW Internal Testing (FIT). The FIT team can accomplish this with simulation software and the MSL Test Automation Kit (MTAK). MTAK has the ability to integrate with the Software Simulation Equipment (SSE) and the Mission Processing and Control System (MPCS) software which makes it a powerful tool within the MSL FSW development process. The MSL team must ensure that the rover accomplishes all stages of the mission successfully. Due to the natural complexity of this project there is a strong emphasis on testing, as failure is not an option. The entire mission could be jeopardized if something is overlooked.

  11. Asset Analysis and Operational Concepts for Separation Assurance Flight Testing at Dryden Flight Research Center

    Science.gov (United States)

    Costa, Guillermo J.; Arteaga, Ricardo A.

    2011-01-01

    A preliminary survey of existing separation assurance and collision avoidance advancements, technologies, and efforts has been conducted in order to develop a concept of operations for flight testing autonomous separation assurance at Dryden Flight Research Center. This effort was part of the Unmanned Aerial Systems in the National Airspace System project. The survey focused primarily on separation assurance projects validated through flight testing (including lessons learned), however current forays into the field were also examined. Comparisons between current Dryden flight and range assets were conducted using House of Quality matrices in order to allow project management to make determinations regarding asset utilization for future flight tests. This was conducted in order to establish a body of knowledge of the current collision avoidance landscape, and thus focus Dryden s efforts more effectively towards the providing of assets and test ranges for future flight testing within this research field.

  12. Partnership Opportunities with AFRC for Wireless Systems Flight Testing

    Science.gov (United States)

    Hang, Richard

    2015-01-01

    The presentation will overview the flight test capabilities at NASA Armstrong Flight Research Center (AFRC), to open up partnership collaboration opportunities for Wireless Community to conduct flight testing of aerospace wireless technologies. Also, it will brief the current activities on wireless sensor system at AFRC through SBIR (Small Business Innovation Research) proposals, and it will show the current areas of interest on wireless technologies that AFRC would like collaborate with Wireless Community to further and testing.

  13. Small-scale fixed wing airplane software verification flight test

    Science.gov (United States)

    Miller, Natasha R.

    The increased demand for micro Unmanned Air Vehicles (UAV) driven by military requirements, commercial use, and academia is creating a need for the ability to quickly and accurately conduct low Reynolds Number aircraft design. There exist several open source software programs that are free or inexpensive that can be used for large scale aircraft design, but few software programs target the realm of low Reynolds Number flight. XFLR5 is an open source, free to download, software program that attempts to take into consideration viscous effects that occur at low Reynolds Number in airfoil design, 3D wing design, and 3D airplane design. An off the shelf, remote control airplane was used as a test bed to model in XFLR5 and then compared to flight test collected data. Flight test focused on the stability modes of the 3D plane, specifically the phugoid mode. Design and execution of the flight tests were accomplished for the RC airplane using methodology from full scale military airplane test procedures. Results from flight test were not conclusive in determining the accuracy of the XFLR5 software program. There were several sources of uncertainty that did not allow for a full analysis of the flight test results. An off the shelf drone autopilot was used as a data collection device for flight testing. The precision and accuracy of the autopilot is unknown. Potential future work should investigate flight test methods for small scale UAV flight.

  14. Detection and Identification of Loss of Efficiency Faults of Flight Actuators

    Directory of Open Access Journals (Sweden)

    Ossmann Daniel

    2015-03-01

    Full Text Available We propose linear parameter-varying (LPV model-based approaches to the synthesis of robust fault detection and diagnosis (FDD systems for loss of efficiency (LOE faults of flight actuators. The proposed methods are applicable to several types of parametric (or multiplicative LOE faults such as actuator disconnection, surface damage, actuator power loss or stall loads. For the detection of these parametric faults, advanced LPV-model detection techniques are proposed, which implicitly provide fault identification information. Fast detection of intermittent stall loads (seen as nuisances, rather than faults is important in enhancing the performance of various fault detection schemes dealing with large input signals. For this case, a dedicated fast identification algorithm is devised. The developed FDD systems are tested on a nonlinear actuator model which is implemented in a full nonlinear aircraft simulation model. This enables the validation of the FDD system’s detection and identification characteristics under realistic conditions.

  15. Flight Test of an L(sub 1) Adaptive Controller on the NASA AirSTAR Flight Test Vehicle

    Science.gov (United States)

    Gregory, Irene M.; Xargay, Enric; Cao, Chengyu; Hovakimyan, Naira

    2010-01-01

    This paper presents results of a flight test of the L-1 adaptive control architecture designed to directly compensate for significant uncertain cross-coupling in nonlinear systems. The flight test was conducted on the subscale turbine powered Generic Transport Model that is an integral part of the Airborne Subscale Transport Aircraft Research system at the NASA Langley Research Center. The results presented are for piloted tasks performed during the flight test.

  16. Flight Test Guide (Part 61 Revised): Instrument Pilot: Helicopter.

    Science.gov (United States)

    Federal Aviation Administration (DOT), Washington, DC. Flight Standards Service.

    The guide provides an outline of the skills required to pass the flight test for an Instrument Pilot Helicopter Rating under Part 61 (revised) of Federal Aviation Regulations. General procedures for flight tests are described and the following pilot operations outlined: maneuvering by reference to instruments, IFR navigation, instrument…

  17. Flight testing of a luminescent surface pressure sensor

    Science.gov (United States)

    Mclachlan, B. G.; Bell, J. H.; Espina, J.; Gallery, J.; Gouterman, M.; Demandante, C. G. N.; Bjarke, L.

    1992-01-01

    NASA ARC has conducted flight tests of a new type of aerodynamic pressure sensor based on a luminescent surface coating. Flights were conducted at the NASA ARC-Dryden Flight Research Facility. The luminescent pressure sensor is based on a surface coating which, when illuminated with ultraviolet light, emits visible light with an intensity dependent on the local air pressure on the surface. This technique makes it possible to obtain pressure data over the entire surface of an aircraft, as opposed to conventional instrumentation, which can only make measurements at pre-selected points. The objective of the flight tests was to evaluate the effectiveness and practicality of a luminescent pressure sensor in the actual flight environment. A luminescent pressure sensor was installed on a fin, the Flight Test Fixture (FTF), that is attached to the underside of an F-104 aircraft. The response of one particular surface coating was evaluated at low supersonic Mach numbers (M = 1.0-1.6) in order to provide an initial estimate of the sensor's capabilities. This memo describes the test approach, the techniques used, and the pressure sensor's behavior under flight conditions. A direct comparison between data provided by the luminescent pressure sensor and that produced by conventional pressure instrumentation shows that the luminescent sensor can provide quantitative data under flight conditions. However, the test results also show that the sensor has a number of limitations which must be addressed if this technique is to prove useful in the flight environment.

  18. Cassini's Test Methodology for Flight Software Verification and Operations

    Science.gov (United States)

    Wang, Eric; Brown, Jay

    2007-01-01

    The Cassini spacecraft was launched on 15 October 1997 on a Titan IV-B launch vehicle. The spacecraft is comprised of various subsystems, including the Attitude and Articulation Control Subsystem (AACS). The AACS Flight Software (FSW) and its development has been an ongoing effort, from the design, development and finally operations. As planned, major modifications to certain FSW functions were designed, tested, verified and uploaded during the cruise phase of the mission. Each flight software upload involved extensive verification testing. A standardized FSW testing methodology was used to verify the integrity of the flight software. This paper summarizes the flight software testing methodology used for verifying FSW from pre-launch through the prime mission, with an emphasis on flight experience testing during the first 2.5 years of the prime mission (July 2004 through January 2007).

  19. Simulation to Flight Test for a UAV Controls Testbed

    Science.gov (United States)

    Motter, Mark A.; Logan, Michael J.; French, Michael L.; Guerreiro, Nelson M.

    2006-01-01

    The NASA Flying Controls Testbed (FLiC) is a relatively small and inexpensive unmanned aerial vehicle developed specifically to test highly experimental flight control approaches. The most recent version of the FLiC is configured with 16 independent aileron segments, supports the implementation of C-coded experimental controllers, and is capable of fully autonomous flight from takeoff roll to landing, including flight test maneuvers. The test vehicle is basically a modified Army target drone, AN/FQM-117B, developed as part of a collaboration between the Aviation Applied Technology Directorate (AATD) at Fort Eustis, Virginia and NASA Langley Research Center. Several vehicles have been constructed and collectively have flown over 600 successful test flights, including a fully autonomous demonstration at the Association of Unmanned Vehicle Systems International (AUVSI) UAV Demo 2005. Simulations based on wind tunnel data are being used to further develop advanced controllers for implementation and flight test.

  20. Small UAS Test Area at NASA's Dryden Flight Research Center

    Science.gov (United States)

    Bauer, Jeffrey T.

    2008-01-01

    This viewgraph presentation reviews the areas that Dryden Flight Research Center has set up for testing small Unmanned Aerial Systems (UAS). It also reviews the requirements and process to use an area for UAS test.

  1. Flight Test Implementation of a Second Generation Intelligent Flight Control System

    Science.gov (United States)

    Williams-Hayes, Peggy S.

    2005-01-01

    The NASA F-15 Intelligent Flight Control System project team has developed a series of flight control concepts designed to demonstrate the benefits of a neural network-based adaptive controller. The objective of the team was to develop and flight-test control systems that use neural network technology, to optimize the performance of the aircraft under nominal conditions, and to stabilize the aircraft under failure conditions. Failure conditions include locked or failed control surfaces as well as unforeseen damage that might occur to the aircraft in flight. The Intelligent Flight Control System team is currently in the process of implementing a second generation control scheme, collectively known as Generation 2 or Gen 2, for flight testing on the NASA F-15 aircraft. This report describes the Gen 2 system as implemented by the team for flight test evaluation. Simulation results are shown which describe the experiment to be performed in flight and highlight the ways in which the Gen 2 system meets the defined objectives.

  2. Flight Test and Handling Qualities Analysis of a Longitudinal Flight Control System Using Multiobjective Techniques

    National Research Council Canada - National Science Library

    Anderson, John

    1998-01-01

    ...) and AFIT MXTOOLS toolboxes were used to produce the optimal, multiobjective designs. These designs were implemented for flight test on the Calspan VSS I Learjet, simulating the unstable longitudinal dynamics of an F-16 type aircraft...

  3. Atmospheric Measurements for Flight Test at NASAs Neil A. Armstrong Flight Research Center

    Science.gov (United States)

    Teets, Edward H.

    2016-01-01

    Information enclosed is to be shared with students of Atmospheric Sciences, Engineering and High School STEM programs. Information will show the relationship between atmospheric Sciences and aeronautical flight testing.

  4. Flight Test of L1 Adaptive Control Law: Offset Landings and Large Flight Envelope Modeling Work

    Science.gov (United States)

    Gregory, Irene M.; Xargay, Enric; Cao, Chengyu; Hovakimyan, Naira

    2011-01-01

    This paper presents new results of a flight test of the L1 adaptive control architecture designed to directly compensate for significant uncertain cross-coupling in nonlinear systems. The flight test was conducted on the subscale turbine powered Generic Transport Model that is an integral part of the Airborne Subscale Transport Aircraft Research system at the NASA Langley Research Center. The results presented include control law evaluation for piloted offset landing tasks as well as results in support of nonlinear aerodynamic modeling and real-time dynamic modeling of the departure-prone edges of the flight envelope.

  5. Subsonic Glideback Rocket Demonstrator Flight Testing

    Science.gov (United States)

    DeTurris, Dianne J.; Foster, Trevor J.; Barthel, Paul E.; Macy, Daniel J.; Droney, Christopher K.; Talay, Theodore A. (Technical Monitor)

    2001-01-01

    For the past two years, Cal Poly's rocket program has been aggressively exploring the concept of remotely controlled, fixed wing, flyable rocket boosters. This program, embodied by a group of student engineers known as Cal Poly Space Systems, has successfully demonstrated the idea of a rocket design that incorporates a vertical launch pattern followed by a horizontal return flight and landing. Though the design is meant for supersonic flight, CPSS demonstrators are deployed at a subsonic speed. Many steps have been taken by the club that allowed the evolution of the StarBooster prototype to reach its current size: a ten-foot tall, one-foot diameter, composite material rocket. Progress is currently being made that involves multiple boosters along with a second stage, third rocket.

  6. Testing Microgravity Flight Hardware Concepts on the NASA KC-135

    Science.gov (United States)

    Motil, Susan M.; Harrivel, Angela R.; Zimmerli, Gregory A.

    2001-01-01

    This paper provides an overview of utilizing the NASA KC-135 Reduced Gravity Aircraft for the Foam Optics and Mechanics (FOAM) microgravity flight project. The FOAM science requirements are summarized, and the KC-135 test-rig used to test hardware concepts designed to meet the requirements are described. Preliminary results regarding foam dispensing, foam/surface slip tests, and dynamic light scattering data are discussed in support of the flight hardware development for the FOAM experiment.

  7. Orion Exploration Flight Test Reaction Control System Jet Interaction Heating Environment from Flight Data

    Science.gov (United States)

    White, Molly E.; Hyatt, Andrew J.

    2016-01-01

    The Orion Multi-Purpose Crew Vehicle (MPCV) Reaction Control System (RCS) is critical to guide the vehicle along the desired trajectory during re-­-entry. However, this system has a significant impact on the convective heating environment to the spacecraft. Heating augmentation from the jet interaction (JI) drives thermal protection system (TPS) material selection and thickness requirements for the spacecraft. This paper describes the heating environment from the RCS on the afterbody of the Orion MPCV during Orion's first flight test, Exploration Flight Test 1 (EFT-1). These jet plumes interact with the wake of the crew capsule and cause an increase in the convective heating environment. Not only is there widespread influence from the jet banks, there may also be very localized effects. The firing history during EFT-1 will be summarized to assess which jet bank interaction was measured during flight. Heating augmentation factors derived from the reconstructed flight data will be presented. Furthermore, flight instrumentation across the afterbody provides the highest spatial resolution of the region of influence of the individual jet banks of any spacecraft yet flown. This distribution of heating augmentation across the afterbody will be derived from the flight data. Additionally, trends with possible correlating parameters will be investigated to assist future designs and ground testing programs. Finally, the challenges of measuring JI, applying this data to future flights and lessons learned will be discussed.

  8. Flight Testing an Iced Business Jet for Flight Simulation Model Validation

    Science.gov (United States)

    Ratvasky, Thomas P.; Barnhart, Billy P.; Lee, Sam; Cooper, Jon

    2007-01-01

    A flight test of a business jet aircraft with various ice accretions was performed to obtain data to validate flight simulation models developed through wind tunnel tests. Three types of ice accretions were tested: pre-activation roughness, runback shapes that form downstream of the thermal wing ice protection system, and a wing ice protection system failure shape. The high fidelity flight simulation models of this business jet aircraft were validated using a software tool called "Overdrive." Through comparisons of flight-extracted aerodynamic forces and moments to simulation-predicted forces and moments, the simulation models were successfully validated. Only minor adjustments in the simulation database were required to obtain adequate match, signifying the process used to develop the simulation models was successful. The simulation models were implemented in the NASA Ice Contamination Effects Flight Training Device (ICEFTD) to enable company pilots to evaluate flight characteristics of the simulation models. By and large, the pilots confirmed good similarities in the flight characteristics when compared to the real airplane. However, pilots noted pitch up tendencies at stall with the flaps extended that were not representative of the airplane and identified some differences in pilot forces. The elevator hinge moment model and implementation of the control forces on the ICEFTD were identified as a driver in the pitch ups and control force issues, and will be an area for future work.

  9. The Max Launch Abort System - Concept, Flight Test, and Evolution

    Science.gov (United States)

    Gilbert, Michael G.

    2014-01-01

    The NASA Engineering and Safety Center (NESC) is an independent engineering analysis and test organization providing support across the range of NASA programs. In 2007 NASA was developing the launch escape system for the Orion spacecraft that was evolved from the traditional tower-configuration escape systems used for the historic Mercury and Apollo spacecraft. The NESC was tasked, as a programmatic risk-reduction effort to develop and flight test an alternative to the Orion baseline escape system concept. This project became known as the Max Launch Abort System (MLAS), named in honor of Maxime Faget, the developer of the original Mercury escape system. Over the course of approximately two years the NESC performed conceptual and tradeoff analyses, designed and built full-scale flight test hardware, and conducted a flight test demonstration in July 2009. Since the flight test, the NESC has continued to further develop and refine the MLAS concept.

  10. Remembering the Musi - SilkAir Flight MI 185 crash victim identification.

    Science.gov (United States)

    Tan, Peng Hui; Wee, Keng Poh; Sahelangi, Peter

    2007-10-01

    On 19 December 1997, SilkAir Flight MI 185, a Boeing B737-300 airliner crashed into the Musi River near Palembang, Southern Sumatra, enroute from Jakarta, Indonesia to Singapore. All 104 passengers and crew onboard were killed. Of the human remains recovered, 6 positive identifications were made, including that of one Singaporean. Two of the identifications were by dental records, 2 by fingerprints, 1 by age estimation and 1 by personal effects. This paper describes the crash victim identification of Flight MI 185. The authors were part of an Indonesia- Singapore forensic team deployed for 3 weeks in Palembang to assist the Indonesian authorities in human remains identification.

  11. Fused Reality for Enhanced Flight Test Capabilities, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — In terms of relevancy to piloted evaluation, there remains no substitute for actual flight tests even when considering the fidelity and effectiveness of modern...

  12. A Flight Control System Architecture for the NASA AirSTAR Flight Test Infrastructure

    Science.gov (United States)

    Murch, Austin M.

    2008-01-01

    A flight control system architecture for the NASA AirSTAR infrastructure has been designed to address the challenges associated with safe and efficient flight testing of research control laws in adverse flight conditions. The AirSTAR flight control system provides a flexible framework that enables NASA Aviation Safety Program research objectives, and includes the ability to rapidly integrate and test research control laws, emulate component or sensor failures, inject automated control surface perturbations, and provide a baseline control law for comparison to research control laws and to increase operational efficiency. The current baseline control law uses an angle of attack command augmentation system for the pitch axis and simple stability augmentation for the roll and yaw axes.

  13. Flight test of the X-29A at high angle of attack: Flight dynamics and controls

    Science.gov (United States)

    Bauer, Jeffrey E.; Clarke, Robert; Burken, John J.

    1995-01-01

    The NASA Dryden Flight Research Center has flight tested two X-29A aircraft at low and high angles of attack. The high-angle-of-attack tests evaluate the feasibility of integrated X-29A technologies. More specific objectives focus on evaluating the high-angle-of-attack flying qualities, defining multiaxis controllability limits, and determining the maximum pitch-pointing capability. A pilot-selectable gain system allows examination of tradeoffs in airplane stability and maneuverability. Basic fighter maneuvers provide qualitative evaluation. Bank angle captures permit qualitative data analysis. This paper discusses the design goals and approach for high-angle-of-attack control laws and provides results from the envelope expansion and handling qualities testing at intermediate angles of attack. Comparisons of the flight test results to the predictions are made where appropriate. The pitch rate command structure of the longitudinal control system is shown to be a valid design for high-angle-of-attack control laws. Flight test results show that wing rock amplitude was overpredicted and aileron and rudder effectiveness were underpredicted. Flight tests show the X-29A airplane to be a good aircraft up to 40 deg angle of attack.

  14. Design and flight testing of a nullable compressor face rake

    Science.gov (United States)

    Holzman, J. K.; Payne, G. A.

    1973-01-01

    A compressor face rake with an internal valve arrangement to permit nulling was designed, constructed, and tested in the laboratory and in flight at the NASA Flight Research Center. When actuated by the pilot in flight, the nullable rake allowed the transducer zero shifts to be determined and then subsequently removed during data reduction. Design details, the fabrication technique, the principle of operation, brief descriptions of associated digital zero-correction programs and the qualification tests, and test results are included. Sample flight data show that the zero shifts were large and unpredictable but could be measured in flight with the rake. The rake functioned reliably and as expected during 25 hours of operation under flight environmental conditions and temperatures from 230 K (-46 F) to greater than 430 K (314 F). The rake was nulled approximately 1000 times. The in-flight zero-shift measurement technique, as well as the rake design, was successful and should be useful in future applications, particularly where accurate measurements of both steady-state and dynamic pressures are required under adverse environmental conditions.

  15. Flight testing a propulsion-controlled aircraft emergency flight control system on an F-15 airplane

    Science.gov (United States)

    Burcham, F. W., Jr.; Burken, John; Maine, Trindel A.

    1994-01-01

    Flight tests of a propulsion-controlled aircraft (PCA) system on an F-15 airplane have been conducted at the NASA Dryden Flight Research Center. The airplane was flown with all flight control surfaces locked both in the manual throttles-only mode and in an augmented system mode. In the latter mode, pilot thumbwheel commands and aircraft feedback parameters were used to position the throttles. Flight evaluation results showed that the PCA system can be used to land an airplane that has suffered a major flight control system failure safely. The PCA system was used to recover the F-15 airplane from a severe upset condition, descend, and land. Pilots from NASA, U.S. Air Force, U.S. Navy, and McDonnell Douglas Aerospace evaluated the PCA system and were favorably impressed with its capability. Manual throttles-only approaches were unsuccessful. This paper describes the PCA system operation and testing. It also presents flight test results and pilot comments.

  16. Flight test guidelines for homebuilt and experimental aircraft

    CSIR Research Space (South Africa)

    White, WJ

    2013-04-01

    Full Text Available experience totalling approximately 7,000 flying hours on forty-five different variants of military aircraft, nearly all in the flight-test environment. He holds a Commercial Pilot’s Licence with a Class I test pilot rating. During his twenty-eight years... of military flight-testing, he was involved in the fixed wing fighter programmes and weapons development testing and amongst others, he was the project test pilot on the Cheetah C, the Mirage IIIRZ/Atar 09K50 engine integration and the Mirage F1 fitted...

  17. System Identification Methods for Aircraft Flight Control Development and Validation

    Science.gov (United States)

    1995-10-01

    System-identification methods compose a mathematical model, or series of models, : from measurements of inputs and outputs of dynamic systems. This paper : discusses the use of frequency-domain system-identification methods for the : development and ...

  18. In-flight wind identification and soft landing control for autonomous unmanned powered parafoils

    Science.gov (United States)

    Luo, Shuzhen; Tan, Panlong; Sun, Qinglin; Wu, Wannan; Luo, Haowen; Chen, Zengqiang

    2018-04-01

    For autonomous unmanned powered parafoil, the ability to perform a final flare manoeuvre against the wind direction can allow a considerable reduction of horizontal and vertical velocities at impact, enabling a soft landing for a safe delivery of sensible loads; the lack of knowledge about the surface-layer winds will result in messing up terminal flare manoeuvre. Moreover, unknown or erroneous winds can also prevent the parafoil system from reaching the target area. To realize accurate trajectory tracking and terminal soft landing in the unknown wind environment, an efficient in-flight wind identification method merely using Global Positioning System (GPS) data and recursive least square method is proposed to online identify the variable wind information. Furthermore, a novel linear extended state observation filter is proposed to filter the groundspeed of the powered parafoil system calculated by the GPS information to provide a best estimation of the present wind during flight. Simulation experiments and real airdrop tests demonstrate the great ability of this method to in-flight identify the variable wind field, and it can benefit the powered parafoil system to fulfil accurate tracking control and a soft landing in the unknown wind field with high landing accuracy and strong wind-resistance ability.

  19. Software Considerations for Subscale Flight Testing of Experimental Control Laws

    Science.gov (United States)

    Murch, Austin M.; Cox, David E.; Cunningham, Kevin

    2009-01-01

    The NASA AirSTAR system has been designed to address the challenges associated with safe and efficient subscale flight testing of research control laws in adverse flight conditions. In this paper, software elements of this system are described, with an emphasis on components which allow for rapid prototyping and deployment of aircraft control laws. Through model-based design and automatic coding a common code-base is used for desktop analysis, piloted simulation and real-time flight control. The flight control system provides the ability to rapidly integrate and test multiple research control laws and to emulate component or sensor failures. Integrated integrity monitoring systems provide aircraft structural load protection, isolate the system from control algorithm failures, and monitor the health of telemetry streams. Finally, issues associated with software configuration management and code modularity are briefly discussed.

  20. Wind and Wake Sensing with UAV Formation Flight: System Development and Flight Testing

    Science.gov (United States)

    Larrabee, Trenton Jameson

    sensing data using UAVs in formation flight. This has been achieved and well documented before in manned aircraft but very little work has been done on UAV wake sensing especially during flight testing. This document describes the development and flight testing of small unmanned aerial system (UAS) for wind and wake sensing purpose including a Ground Control Station (GCS) and UAVs. This research can be stated in four major components. Firstly, formation flight was obtained by integrating a formation flight controller on the WVU Phastball Research UAV aircraft platform from the Flight Control Systems Laboratory (FCSL) at West Virginia University (WVU). Second, a new approach to wind estimation using an Unscented Kalman filter (UKF) is discussed along with results from flight data. Third, wake modeling within a simulator and wake sensing during formation flight is shown. Finally, experimental results are used to discuss the "sweet spot" for energy harvesting in formation flight, a novel approach to cooperative wind estimation, and gust suppression control for a follower aircraft in formation flight.

  1. Space Shuttle Boundary Layer Transition Flight Experiment Ground Testing Overview

    Science.gov (United States)

    Berger, Karen T.; Anderson, Brian P.; Campbell, Charles H.

    2014-01-01

    In support of the Boundary Layer Transition (BLT) Flight Experiment (FE) Project in which a manufactured protuberance tile was installed on the port wing of Space Shuttle Orbiter Discovery for STS-119, STS- 128, STS-131 and STS-133 as well as Space Shuttle Orbiter Endeavour for STS-134, a significant ground test campaign was completed. The primary goals of the test campaign were to provide ground test data to support the planning and safety certification efforts required to fly the flight experiment as well as validation for the collected flight data. These test included Arcjet testing of the tile protuberance, aerothermal testing to determine the boundary layer transition behavior and resultant surface heating and planar laser induced fluorescence (PLIF) testing in order to gain a better understanding of the flow field characteristics associated with the flight experiment. This paper provides an overview of the BLT FE Project ground testing. High-level overviews of the facilities, models, test techniques and data are presented, along with a summary of the insights gained from each test.

  2. The use of an automated flight test management system in the development of a rapid-prototyping flight research facility

    Science.gov (United States)

    Duke, Eugene L.; Hewett, Marle D.; Brumbaugh, Randal W.; Tartt, David M.; Antoniewicz, Robert F.; Agarwal, Arvind K.

    1988-01-01

    An automated flight test management system (ATMS) and its use to develop a rapid-prototyping flight research facility for artificial intelligence (AI) based flight systems concepts are described. The ATMS provides a flight test engineer with a set of tools that assist in flight planning and simulation. This system will be capable of controlling an aircraft during the flight test by performing closed-loop guidance functions, range management, and maneuver-quality monitoring. The rapid-prototyping flight research facility is being developed at the Dryden Flight Research Facility of the NASA Ames Research Center (Ames-Dryden) to provide early flight assessment of emerging AI technology. The facility is being developed as one element of the aircraft automation program which focuses on the qualification and validation of embedded real-time AI-based systems.

  3. Human factors quantification via boundary identification of flight performance margin

    Directory of Open Access Journals (Sweden)

    Yang Changpeng

    2014-08-01

    Full Text Available A systematic methodology including a computational pilot model and a pattern recognition method is presented to identify the boundary of the flight performance margin for quantifying the human factors. The pilot model is proposed to correlate a set of quantitative human factors which represent the attributes and characteristics of a group of pilots. Three information processing components which are influenced by human factors are modeled: information perception, decision making, and action execution. By treating the human factors as stochastic variables that follow appropriate probability density functions, the effects of human factors on flight performance can be investigated through Monte Carlo (MC simulation. Kernel density estimation algorithm is selected to find and rank the influential human factors. Subsequently, human factors are quantified through identifying the boundary of the flight performance margin by the k-nearest neighbor (k-NN classifier. Simulation-based analysis shows that flight performance can be dramatically improved with the quantitative human factors.

  4. Acoustic flight test of the Piper Lance

    Science.gov (United States)

    1986-12-01

    Research is being conducted to refine current noise regulation of propeller-driven small airplanes. Studies are examining the prospect of a substituting a takeoff procedure of equal stringency for the level flyover certification test presently requir...

  5. Artificial intelligence and expert systems in-flight software testing

    Science.gov (United States)

    Demasie, M. P.; Muratore, J. F.

    1991-01-01

    The authors discuss the introduction of advanced information systems technologies such as artificial intelligence, expert systems, and advanced human-computer interfaces directly into Space Shuttle software engineering. The reconfiguration automation project (RAP) was initiated to coordinate this move towards 1990s software technology. The idea behind RAP is to automate several phases of the flight software testing procedure and to introduce AI and ES into space shuttle flight software testing. In the first phase of RAP, conventional tools to automate regression testing have already been developed or acquired. There are currently three tools in use.

  6. Design and utilization of a Flight Test Engineering Database Management System at the NASA Dryden Flight Research Facility

    Science.gov (United States)

    Knighton, Donna L.

    1992-01-01

    A Flight Test Engineering Database Management System (FTE DBMS) was designed and implemented at the NASA Dryden Flight Research Facility. The X-29 Forward Swept Wing Advanced Technology Demonstrator flight research program was chosen for the initial system development and implementation. The FTE DBMS greatly assisted in planning and 'mass production' card preparation for an accelerated X-29 research program. Improved Test Plan tracking and maneuver management for a high flight-rate program were proven, and flight rates of up to three flights per day, two times per week were maintained.

  7. A model structure for identification of linear models of the UH-60 helicopter in hover and forward flight

    Science.gov (United States)

    1995-08-01

    A linear model structure applicable to identification of the UH-60 flight : dynamics in hover and forward flight without rotor-state data is developed. The : structure of the model is determined through consideration of the important : dynamic modes ...

  8. Low Density Supersonic Decelerator Flight Dynamics Test-1 Flight Design and Targeting

    Science.gov (United States)

    Ivanov, Mark

    2015-01-01

    NASA's Low Density Supersonic Decelerator (LDSD) program was established to identify, develop, and eventually qualify to Test [i.e. Technology] Readiness Level (TRL) - 6 aerodynamic decelerators for eventual use on Mars. Through comprehensive Mars application studies, two distinct Supersonic Inflatable Aerodynamic Decelerator (SIAD) designs were chosen that afforded the optimum balance of benefit, cost, and development risk. In addition, a Supersonic Disk Sail (SSDS) parachute design was chosen that satisfied the same criteria. The final phase of the multi-tiered qualification process involves Earth Supersonic Flight Dynamics Tests (SFDTs) within environmental conditions similar to those that would be experienced during a Mars Entry, Descent, and Landing (EDL) mission. The first of these flight tests (i.e. SFDT-1) was completed on June 28, 2014 with two more tests scheduled for the summer of 2015 and 2016, respectively. The basic flight design for all the SFDT flights is for the SFDT test vehicle to be ferried to a float altitude of 120 kilo-feet by a 34 thousand cubic feet (Mcf) heavy lift helium balloon. Once float altitude is reached, the test vehicle is released from the balloon, spun-up for stability, and accelerated to supersonic speeds using a Star48 solid rocket motor. After burnout of the Star48 motor the vehicle decelerates to pre-flight selected test conditions for the deployment of the SIAD system. After further deceleration with the SIAD deployed, the SSDS parachute is then deployed stressing the performance of the parachute in the wake of the SIAD augmented blunt body. The test vehicle/SIAD/parachute system then descends to splashdown in the Pacific Ocean for eventual recovery. This paper will discuss the development of both the test vehicle and the trajectory sequence including design trade-offs resulting from the interaction of both engineering efforts. In addition, the SFDT-1 nominal trajectory design and associated sensitivities will be discussed

  9. UAV Research, Operations, and Flight Test at the NASA Dryden Flight Research Center

    Science.gov (United States)

    Cosentino, Gary B.

    2009-01-01

    This slide presentation reviews some of the projects that have extended NASA Dryden's capabilities in designing, testing, and using Unmanned Aerial Vehicles (UAV's). Some of the UAV's have been for Science and experimental applications, some have been for flight research and demonstration purposes, and some have been small UAV's for other customers.

  10. Helicopter Acoustic Flight Test with Altitude Variation and Maneuvers

    Science.gov (United States)

    Watts, Michael E.; Greenwood, Eric; Sim, Ben; Stephenson, James; Smith, Charles D.

    2016-01-01

    A cooperative flight test campaign between NASA and the U.S. Army was performed from September 2014 to February 2015. The purposes of the testing were to: investigate the effects of altitude variation on noise generation, investigate the effects of gross weight variation on noise generation, establish the statistical variability in acoustic flight testing of helicopters, and characterize the effects of transient maneuvers on radiated noise for a medium-lift utility helicopter. This test was performed at three test sites (0, 4000, and 7000 feet above mean sea level) with two aircraft (AS350 SD1 and EH-60L) tested at each site. This report provides an overview of the test, documents the data acquired and describes the formats of the stored data.

  11. Armstrong Flight Research Center Flight Test Capabilities and Opportunities for the Applications of Wireless Data Acquisition Systems

    Science.gov (United States)

    Hang, Richard

    2015-01-01

    The presentation will overview NASA Armstrong Flight Research Centers flight test capabilities, which can provide various means for flight testing of passive and active wireless sensor systems, also, it will address the needs of the wireless data acquisition solutions for the centers flight instrumentation issues such as additional weight caused by added instrumentation wire bundles, connectors, wire cables routing, moving components, etc., that the Passive Wireless Sensor Technology Workshop may help. The presentation shows the constraints and requirements that the wireless sensor systems will face in the flight test applications.

  12. Flight test of a resident backup software system

    Science.gov (United States)

    Deets, Dwain A.; Lock, Wilton P.; Megna, Vincent A.

    1987-01-01

    A new fault-tolerant system software concept employing the primary digital computers as host for the backup software portion has been implemented and flight tested in the F-8 digital fly-by-wire airplane. The system was implemented in such a way that essentially no transients occurred in transferring from primary to backup software. This was accomplished without a significant increase in the complexity of the backup software. The primary digital system was frame synchronized, which provided several advantages in implementing the resident backup software system. Since the time of the flight tests, two other flight vehicle programs have made a commitment to incorporate resident backup software similar in nature to the system described here.

  13. Flight Test Results for the F-16XL With a Digital Flight Control System

    Science.gov (United States)

    Stachowiak, Susan J.; Bosworth, John T.

    2004-01-01

    In the early 1980s, two F-16 airplanes were modified to extend the fuselage length and incorporate a large area delta wing planform. These two airplanes, designated the F-16XL, were designed by the General Dynamics Corporation (now Lockheed Martin Tactical Aircraft Systems) (Fort Worth, Texas) and were prototypes for a derivative fighter evaluation program conducted by the United States Air Force. Although the concept was never put into production, the F-16XL prototypes provided a unique planform for testing concepts in support of future high-speed supersonic transport aircraft. To extend the capabilities of this testbed vehicle the F-16XL ship 1 aircraft was upgraded with a digital flight control system. The added flexibility of a digital flight control system increases the versatility of this airplane as a testbed for aerodynamic research and investigation of advanced technologies. This report presents the handling qualities flight test results covering the envelope expansion of the F-16XL with the digital flight control system.

  14. Aerodynamic Reconstruction Applied to Parachute Test Vehicle Flight Data Analysis

    Science.gov (United States)

    Cassady, Leonard D.; Ray, Eric S.; Truong, Tuan H.

    2013-01-01

    The aerodynamics, both static and dynamic, of a test vehicle are critical to determining the performance of the parachute cluster in a drop test and for conducting a successful test. The Capsule Parachute Assembly System (CPAS) project is conducting tests of NASA's Orion Multi-Purpose Crew Vehicle (MPCV) parachutes at the Army Yuma Proving Ground utilizing the Parachute Test Vehicle (PTV). The PTV shape is based on the MPCV, but the height has been reduced in order to fit within the C-17 aircraft for extraction. Therefore, the aerodynamics of the PTV are similar, but not the same as, the MPCV. A small series of wind tunnel tests and computational fluid dynamics cases were run to modify the MPCV aerodynamic database for the PTV, but aerodynamic reconstruction of the flights has proven an effective source for further improvements to the database. The acceleration and rotational rates measured during free flight, before parachute inflation but during deployment, were used to con rm vehicle static aerodynamics. A multibody simulation is utilized to reconstruct the parachute portions of the flight. Aerodynamic or parachute parameters are adjusted in the simulation until the prediction reasonably matches the flight trajectory. Knowledge of the static aerodynamics is critical in the CPAS project because the parachute riser load measurements are scaled based on forebody drag. PTV dynamic damping is critical because the vehicle has no reaction control system to maintain attitude - the vehicle dynamics must be understood and modeled correctly before flight. It will be shown here that aerodynamic reconstruction has successfully contributed to the CPAS project.

  15. HIFiRE-5 Flight Test Preliminary Results (Postprint)

    Science.gov (United States)

    2013-11-01

    CFD . 15. SUBJECT TERMS Boundary layer transition, hypersonic , flight test 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT: SAR 18...consistent with prior CFD 33,34 and wind tunnel measurements 28,29 at hypersonic conditions that indicated that the centerline is more unstable... Hypersonic Sciences Branch High Speed Systems Division NOVEMBER 2013 Approved for public release; distribution unlimited

  16. Maneuver Acoustic Flight Test of the Bell 430 Helicopter

    Science.gov (United States)

    Watts, Michael E.; Snider, Royce; Greenwood, Eric; Baden, Joel

    2012-01-01

    A cooperative flight test by NASA, Bell Helicopter and the U.S. Army to characterize the steady state acoustics and measure the maneuver noise of a Bell Helicopter 430 aircraft was accomplished. The test occurred during June/July, 2011 at Eglin Air Force Base, Florida. This test gathered a total of 410 data points over 10 test days and compiled an extensive data base of dynamic maneuver measurements. Three microphone configurations with up to 31 microphones in each configuration were used to acquire acoustic data. Aircraft data included DGPS, aircraft state and rotor state information. This paper provides an overview of the test.

  17. A stochastic global identification framework for aerospace structures operating under varying flight states

    Science.gov (United States)

    Kopsaftopoulos, Fotis; Nardari, Raphael; Li, Yu-Hung; Chang, Fu-Kuo

    2018-01-01

    In this work, a novel data-based stochastic "global" identification framework is introduced for aerospace structures operating under varying flight states and uncertainty. In this context, the term "global" refers to the identification of a model that is capable of representing the structure under any admissible flight state based on data recorded from a sample of these states. The proposed framework is based on stochastic time-series models for representing the structural dynamics and aeroelastic response under multiple flight states, with each state characterized by several variables, such as the airspeed, angle of attack, altitude and temperature, forming a flight state vector. The method's cornerstone lies in the new class of Vector-dependent Functionally Pooled (VFP) models which allow the explicit analytical inclusion of the flight state vector into the model parameters and, hence, system dynamics. This is achieved via the use of functional data pooling techniques for optimally treating - as a single entity - the data records corresponding to the various flight states. In this proof-of-concept study the flight state vector is defined by two variables, namely the airspeed and angle of attack of the vehicle. The experimental evaluation and assessment is based on a prototype bio-inspired self-sensing composite wing that is subjected to a series of wind tunnel experiments under multiple flight states. Distributed micro-sensors in the form of stretchable sensor networks are embedded in the composite layup of the wing in order to provide the sensing capabilities. Experimental data collected from piezoelectric sensors are employed for the identification of a stochastic global VFP model via appropriate parameter estimation and model structure selection methods. The estimated VFP model parameters constitute two-dimensional functions of the flight state vector defined by the airspeed and angle of attack. The identified model is able to successfully represent the wing

  18. Application of a flight test and data analysis technique to flutter of a drone aircraft

    Science.gov (United States)

    Bennett, R. M.

    1981-01-01

    Modal identification results presented were obtained from recent flight flutter tests of a drone vehicle with a research wing (DAST ARW-1 for Drones for Aerodynamic and Structural Testing, Aeroelastic Research Wing-1). This vehicle is equipped with an active flutter suppression system (FSS). Frequency and damping of several modes are determined by a time domain modal analysis of the impulse response function obtained by Fourier transformations of data from fast swept sine wave excitation by the FSS control surface on the wing. Flutter points are determined for two different altitudes with the FSS off. Data are given for near the flutter boundary with the FSS on.

  19. Ultrasonic testing using time of flight diffraction technique (TOFD)

    International Nuclear Information System (INIS)

    Khurram Shahzad; Ahmad Mirza Safeer Ahmad; Muhammad Asif Khan

    2009-04-01

    This paper describes the ultrasonic testing using Time Flight Diffraction (TOFD) Technique for welded samples having different types and sizes of defects. TOFD is a computerized ultrasonic system, able to scan, store and evaluate indications in terms of location, through thickness and length in a more easy and convenient. Time of Flight Diffraction Technique (TOFD) is more fast and easy technique for ultrasonic testing as we can examine a weld i a single scan along the length of the weld with two probes known as D-scan. It shows the image of the complete weld with the defect information. The examinations were performed on carbon steel samples used for ultrasonic testing using 70 degree probes. The images for different type of defects were obtained. (author)

  20. Ares-I-X Stability and Control Flight Test: Analysis and Plans

    Science.gov (United States)

    Brandon, Jay M.; Derry, Stephen D.; Heim, Eugene H.; Hueschen, Richard M.; Bacon, Barton J.

    2008-01-01

    The flight test of the Ares I-X vehicle provides a unique opportunity to reduce risk of the design of the Ares I vehicle and test out design, math modeling, and analysis methods. One of the key features of the Ares I design is the significant static aerodynamic instability coupled with the relatively flexible vehicle - potentially resulting in a challenging controls problem to provide adequate flight path performance while also providing adequate structural mode damping and preventing adverse control coupling to the flexible structural modes. Another challenge is to obtain enough data from the single flight to be able to conduct analysis showing the effectiveness of the controls solutions and have data to inform design decisions for Ares I. This paper will outline the modeling approaches and control system design to conduct this flight test, and also the system identification techniques developed to extract key information such as control system performance (gain/phase margins, for example), structural dynamics responses, and aerodynamic model estimations.

  1. Deployment Testing of the De-Orbit Sail Flight Hardware

    OpenAIRE

    Hillebrandt, Martin; Meyer, Sebastian; Zander, Martin; Hühne, Christian

    2015-01-01

    The paper describes the results of the deployment testing of the De-Orbit Sail flight hardware, a drag sail for de-orbiting applications, performed by DLR. It addresses in particular the deployment tests of the fullscale sail subsystem and deployment force tests performed on the boom deployment module. For the fullscale sail testing a gravity compensation device is used which is described in detail. It allows observations of the in-plane interaction of the booms with the sail membrane and the...

  2. Environmental Tests of the Flight GLAST LAT Tracker Towers

    Energy Technology Data Exchange (ETDEWEB)

    Bagagli, R.; Baldini, L.; Bellazzini, R.; Barbiellini, G.; Belli, F.; Borden, T.; Brez, A.; Brigida, M.; Caliandro, G.A.; Cecchi, C.; Cohen-Tanugi, J.; Angelis, A.De; Drell, P.; Favuzzi, C.; Fusco, P.; Gargano, F.; Germani, S.; Giglietto, N.; Giordano, F.; Goodman, J.; Himel, T.

    2008-03-12

    The Gamma-ray Large Area Space telescope (GLAST) is a gamma-ray satellite scheduled for launch in 2008. Before the assembly of the Tracker subsystem of the Large Area Telescope (LAT) science instrument of GLAST, every component (tray) and module (tower) has been subjected to extensive ground testing required to ensure successful launch and on-orbit operation. This paper describes the sequence and results of the environmental tests performed on an engineering model and all the flight hardware of the GLAST LAT Tracker. Environmental tests include vibration testing, thermal cycles and thermal-vacuum cycles of every tray and tower as well as the verification of their electrical performance.

  3. Navigation and flight director guidance for the NASA/FAA helicopter MLS curved approach flight test program

    Science.gov (United States)

    Phatak, A. V.; Lee, M. G.

    1985-01-01

    The navigation and flight director guidance systems implemented in the NASA/FAA helicopter microwave landing system (MLS) curved approach flight test program is described. Flight test were conducted at the U.S. Navy's Crows Landing facility, using the NASA Ames UH-lH helicopter equipped with the V/STOLAND avionics system. The purpose of these tests was to investigate the feasibility of flying complex, curved and descending approaches to a landing using MLS flight director guidance. A description of the navigation aids used, the avionics system, cockpit instrumentation and on-board navigation equipment used for the flight test is provided. Three generic reference flight paths were developed and flown during the test. They were as follows: U-Turn, S-turn and Straight-In flight profiles. These profiles and their geometries are described in detail. A 3-cue flight director was implemented on the helicopter. A description of the formulation and implementation of the flight director laws is also presented. Performance data and analysis is presented for one pilot conducting the flight director approaches.

  4. Development and Flight Testing of a Neural Network Based Flight Control System on the NF-15B Aircraft

    Science.gov (United States)

    Bomben, Craig R.; Smolka, James W.; Bosworth, John T.; Silliams-Hayes, Peggy S.; Burken, John J.; Larson, Richard R.; Buschbacher, Mark J.; Maliska, Heather A.

    2006-01-01

    The Intelligent Flight Control System (IFCS) project at the NASA Dryden Flight Research Center, Edwards AFB, CA, has been investigating the use of neural network based adaptive control on a unique NF-15B test aircraft. The IFCS neural network is a software processor that stores measured aircraft response information to dynamically alter flight control gains. In 2006, the neural network was engaged and allowed to learn in real time to dynamically alter the aircraft handling qualities characteristics in the presence of actual aerodynamic failure conditions injected into the aircraft through the flight control system. The use of neural network and similar adaptive technologies in the design of highly fault and damage tolerant flight control systems shows promise in making future aircraft far more survivable than current technology allows. This paper will present the results of the IFCS flight test program conducted at the NASA Dryden Flight Research Center in 2006, with emphasis on challenges encountered and lessons learned.

  5. Test Plan for Cask Identification Detector

    Energy Technology Data Exchange (ETDEWEB)

    Rauch, Eric Benton [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-09-29

    This document serves to outline the testing of a Used Fuel Cask Identification Detector (CID) currently being designed under the DOE-NE MPACT Campaign. A bench-scale prototype detector will be constructed and tested using surrogate neutron sources. The testing will serve to inform the design of the full detector that is to be used as a way of fingerprinting used fuel storage casks based on the neutron signature produced by the used fuel inside the cask.

  6. Identification of Thrust, Lift, and Drag for Deep-stall Flight Data of a Fixed-wing Unmanned Aircraft

    DEFF Research Database (Denmark)

    Cunis, Torbjørn; Leth, Tobias; Totu, Luminita Cristiana

    2018-01-01

    In this paper, we consider a small unmanned aircraft and data collected during regular and deep-stall flight. We present an identification method for the thrust force generated by the propulsion system based on the in-flight measurements where we make use of the well-known linear and quadratic...... force estimation in the full flight envelope....

  7. Mars Science Laboratory Flight Software Boot Robustness Testing Project Report

    Science.gov (United States)

    Roth, Brian

    2011-01-01

    On the surface of Mars, the Mars Science Laboratory will boot up its flight computers every morning, having charged the batteries through the night. This boot process is complicated, critical, and affected by numerous hardware states that can be difficult to test. The hardware test beds do not facilitate testing a long duration of back-to-back unmanned automated tests, and although the software simulation has provided the necessary functionality and fidelity for this boot testing, there has not been support for the full flexibility necessary for this task. Therefore to perform this testing a framework has been build around the software simulation that supports running automated tests loading a variety of starting configurations for software and hardware states. This implementation has been tested against the nominal cases to validate the methodology, and support for configuring off-nominal cases is ongoing. The implication of this testing is that the introduction of input configurations that have yet proved difficult to test may reveal boot scenarios worth higher fidelity investigation, and in other cases increase confidence in the robustness of the flight software boot process.

  8. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets ▿

    OpenAIRE

    Prod'hom, Guy; Bizzini, Alain; Durussel, Christian; Bille, Jacques; Greub, Gilbert

    2010-01-01

    An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.

  9. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.

    Science.gov (United States)

    Prod'hom, Guy; Bizzini, Alain; Durussel, Christian; Bille, Jacques; Greub, Gilbert

    2010-04-01

    An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.

  10. Model Based Analysis and Test Generation for Flight Software

    Science.gov (United States)

    Pasareanu, Corina S.; Schumann, Johann M.; Mehlitz, Peter C.; Lowry, Mike R.; Karsai, Gabor; Nine, Harmon; Neema, Sandeep

    2009-01-01

    We describe a framework for model-based analysis and test case generation in the context of a heterogeneous model-based development paradigm that uses and combines Math- Works and UML 2.0 models and the associated code generation tools. This paradigm poses novel challenges to analysis and test case generation that, to the best of our knowledge, have not been addressed before. The framework is based on a common intermediate representation for different modeling formalisms and leverages and extends model checking and symbolic execution tools for model analysis and test case generation, respectively. We discuss the application of our framework to software models for a NASA flight mission.

  11. The Building Blocks for JWST I and T (Integrations and Test) to Operations - From Simulator to Flight Units

    Science.gov (United States)

    Fatig, Curtis; Ochs, William; Johns, Alan; Seaton, Bonita; Adams, Cynthia; Wasiak, Francis; Jones, Ronald; Jackson, Wallace

    2012-01-01

    The James Webb Space Telescope (JWST) Project has an extended integration and test (I&T) phase due to long procurement and development times of various components as well as recent launch delays. The JWST Ground Segment and Operations group has developed a roadmap of the various ground and flight elements and their use in the various JWST I&T test programs. The JWST Project s building block approach to the eventual operational systems, while not new, is complex and challenging; a large-scale mission like JWST involves international partners, many vendors across the United States, and competing needs for the same systems. One of the challenges is resource balancing so simulators and flight products for various elements congeal into integrated systems used for I&T and flight operations activities. This building block approach to an incremental buildup provides for early problem identification with simulators and exercises the flight operations systems, products, and interfaces during the JWST I&T test programs. The JWST Project has completed some early I&T with the simulators, engineering models and some components of the operational ground system. The JWST Project is testing the various flight units as they are delivered and will continue to do so for the entire flight and operational system. The JWST Project has already and will continue to reap the value of the building block approach on the road to launch and flight operations.

  12. Pre-Flight Tests with Astronauts, Flight and Ground Hardware, to Assure On-Orbit Success

    Science.gov (United States)

    Haddad Michael E.

    2010-01-01

    On-Orbit Constraints Test (OOCT's) refers to mating flight hardware together on the ground before they will be mated on-orbit or on the Lunar surface. The concept seems simple but it can be difficult to perform operations like this on the ground when the flight hardware is being designed to be mated on-orbit in a zero-g/vacuum environment of space or low-g/vacuum environment on the Lunar/Mars Surface. Also some of the items are manufactured years apart so how are mating tasks performed on these components if one piece is on-orbit/on Lunar/Mars surface before its mating piece is planned to be built. Both the Internal Vehicular Activity (IVA) and Extra-Vehicular Activity (EVA) OOCT's performed at Kennedy Space Center will be presented in this paper. Details include how OOCT's should mimic on-orbit/Lunar/Mars surface operational scenarios, a series of photographs will be shown that were taken during OOCT's performed on International Space Station (ISS) flight elements, lessons learned as a result of the OOCT's will be presented and the paper will conclude with possible applications to Moon and Mars Surface operations planned for the Constellation Program.

  13. High resolution time-of-flight (TOF) detector for particle identification

    Energy Technology Data Exchange (ETDEWEB)

    Boehm, Merlin; Lehmann, Albert; Pfaffinger, Markus; Uhlig, Fred [Physikalisches Institut, Universitaet Erlangen-Nuernberg (Germany); Collaboration: PANDA-Collaboration

    2016-07-01

    Several prototype tests were performed with the PANDA DIRC detectors at the CERN T9 beam line. A mixed hadron beam with pions, kaons and protons was used at momenta from 2 to 10 GeV/c. For these tests a good particle identification was mandatory. We report about a high resolution TOF detector built especially for this purpose. It consists of two stations each consisting of a Cherenkov radiator read out by a Microchannel-Plate Photomultiplier (MCP-PMT) and a Scintillating Tile (SciTil) counter read out by silicon photomultipliers (SiPMs). With a flight path of 29 m a pion/kaon separation up to 5 GeV/c and a pion/proton separation up to 10 GeV/c was obtained. From the TOF resolutions of different counter combinations the time resolution (sigma) of the individual MCP-PMTs and SciTils was determined. The best counter reached a time resolution of 50 ps.

  14. Development and Flight Test of an Augmented Thrust-Only Flight Control System on an MD-11 Transport Airplane

    Science.gov (United States)

    Burcham, Frank W., Jr.; Maine, Trindel A.; Burken, John J.; Pappas, Drew

    1996-01-01

    An emergency flight control system using only engine thrust, called Propulsion-Controlled Aircraft (PCA), has been developed and flight tested on an MD-11 airplane. In this thrust-only control system, pilot flight path and track commands and aircraft feedback parameters are used to control the throttles. The PCA system was installed on the MD-11 airplane using software modifications to existing computers. Flight test results show that the PCA system can be used to fly to an airport and safely land a transport airplane with an inoperative flight control system. In up-and-away operation, the PCA system served as an acceptable autopilot capable of extended flight over a range of speeds and altitudes. The PCA approaches, go-arounds, and three landings without the use of any non-nal flight controls have been demonstrated, including instrument landing system-coupled hands-off landings. The PCA operation was used to recover from an upset condition. In addition, PCA was tested at altitude with all three hydraulic systems turned off. This paper reviews the principles of throttles-only flight control; describes the MD-11 airplane and systems; and discusses PCA system development, operation, flight testing, and pilot comments.

  15. Development and Flight Test of an Emergency Flight Control System Using Only Engine Thrust on an MD-11 Transport Airplane

    Science.gov (United States)

    Burcham, Frank W., Jr.; Burken, John J.; Maine, Trindel A.; Fullerton, C. Gordon

    1997-01-01

    An emergency flight control system that uses only engine thrust, called the propulsion-controlled aircraft (PCA) system, was developed and flight tested on an MD-11 airplane. The PCA system is a thrust-only control system, which augments pilot flightpath and track commands with aircraft feedback parameters to control engine thrust. The PCA system was implemented on the MD-11 airplane using only software modifications to existing computers. Results of a 25-hr flight test show that the PCA system can be used to fly to an airport and safely land a transport airplane with an inoperative flight control system. In up-and-away operation, the PCA system served as an acceptable autopilot capable of extended flight over a range of speeds, altitudes, and configurations. PCA approaches, go-arounds, and three landings without the use of any normal flight controls were demonstrated, including ILS-coupled hands-off landings. PCA operation was used to recover from an upset condition. The PCA system was also tested at altitude with all three hydraulic systems turned off. This paper reviews the principles of throttles-only flight control, a history of accidents or incidents in which some or all flight controls were lost, the MD-11 airplane and its systems, PCA system development, operation, flight testing, and pilot comments.

  16. Marshall Space Flight Center's Impact Testing Facility Capabilities

    Science.gov (United States)

    Finchum, Andy; Hubbs, Whitney; Evans, Steve

    2008-01-01

    Marshall Space Flight Center s (MSFC) Impact Testing Facility (ITF) serves as an important installation for space and missile related materials science research. The ITF was established and began its research in spacecraft debris shielding in the early 1960s, then played a major role in the International Space Station debris shield development. As NASA became more interested in launch debris and in-flight impact concerns, the ITF grew to include research in a variety of impact genres. Collaborative partnerships with the DoD led to a wider range of impact capabilities being relocated to MSFC as a result of the closure of Particle Impact Facilities in Santa Barbara, California. The Particle Impact Facility had a 30 year history in providing evaluations of aerospace materials and components during flights through rain, ice, and solid particle environments at subsonic through hypersonic velocities. The facility s unique capabilities were deemed a "National Asset" by the DoD. The ITF now has capabilities including environmental, ballistic, and hypervelocity impact testing utilizing an array of air, powder, and two-stage light gas guns to accommodate a variety of projectile and target types and sizes. Numerous upgrades including new instrumentation, triggering circuitry, high speed photography, and optimized sabot designs have been implemented. Other recent research has included rain drop demise characterization tests to obtain data for inclusion in on-going model development. The current and proposed ITF capabilities range from rain to micrometeoroids allowing the widest test parameter range possible for materials investigations in support of space, atmospheric, and ground environments. These test capabilities including hydrometeor, single/multi-particle, ballistic gas guns, exploding wire gun, and light gas guns combined with Smooth Particle Hydrodynamics Code (SPHC) simulations represent the widest range of impact test capabilities in the country.

  17. Behavioural system identification of visual flight speed control in Drosophila melanogaster.

    Science.gov (United States)

    Rohrseitz, Nicola; Fry, Steven N

    2011-02-06

    Behavioural control in many animals involves complex mechanisms with intricate sensory-motor feedback loops. Modelling allows functional aspects to be captured without relying on a description of the underlying complex, and often unknown, mechanisms. A wide range of engineering techniques are available for modelling, but their ability to describe time-continuous processes is rarely exploited to describe sensory-motor control mechanisms in biological systems. We performed a system identification of visual flight speed control in the fruitfly Drosophila, based on an extensive dataset of open-loop responses previously measured under free flight conditions. We identified a second-order under-damped control model with just six free parameters that well describes both the transient and steady-state characteristics of the open-loop data. We then used the identified control model to predict flight speed responses after a visual perturbation under closed-loop conditions and validated the model with behavioural measurements performed in free-flying flies under the same closed-loop conditions. Our system identification of the fruitfly's flight speed response uncovers the high-level control strategy of a fundamental flight control reflex without depending on assumptions about the underlying physiological mechanisms. The results are relevant for future investigations of the underlying neuromotor processing mechanisms, as well as for the design of biomimetic robots, such as micro-air vehicles.

  18. Multimodal Displays for Target Localization in a Flight Test

    National Research Council Canada - National Science Library

    Tannen, Robert

    2001-01-01

    ... Synthesized Immersion Research Environment (SIRE) facility. Twelve pilots with a mean of 2652 flight hours performed a simulated flight task in which they were instructed to maintain a prescribed flight path, air speed, and altitude...

  19. LISA and its in-flight test precursor SMART-2

    International Nuclear Information System (INIS)

    Vitale, S.; Bender, P.; Brillet, A.; Buchman, S.; Cavalleri, A.; Cerdonio, M.; Cruise, M.; Cutler, C.; Danzmann, K.; Dolesi, R.; Folkner, W.; Gianolio, A.; Jafry, Y.; Hasinger, G.; Heinzel, G.; Hogan, C.; Hueller, M.; Hough, J.; Phinney, S.; Prince, T.; Richstone, D.; Robertson, D.; Rodrigues, M.; Ruediger, A.; Sandford, M.; Schilling, R.; Shoemaker, D.; Schutz, B.; Stebbins, R.; Stubbs, C.; Sumner, T.; Thorne, K.; Tinto, M.; Touboul, P.; Ward, H.; Weber, W.; Winkler, W.

    2002-01-01

    LISA will be the first space-home gravitational wave observatory. It aims to detect gravitational waves in the 0.1 mHz/1 Hz range from sources including galactic binaries, super-massive black-hole binaries, capture of objects by super-massive black-holes and stochastic background. LISA is an ESA approved Cornerstone Mission foreseen as a joint ESA-NASA endeavour to be launched in 2010-11. The principle of operation of LISA is based on laser ranging of test-masses under pure geodesic motion. Achieving pure geodesic motion at the level requested for LISA, 3x10 -15 ms -2 /√Hz at 0.1 mHz, is considered a challenging technological objective. To reduce the risk, both ESA and NASA are pursuing an in-flight test of the relevant technology. The goal of the test is to demonstrate geodetic motion within one order of magnitude from the LISA performance. ESA has given this test as the primary goal of its technology dedicated mission SMART-2 with a launch in 2006. This paper describes the basics of LISA, its key technologies, and its in-flight precursor test on SMART-2

  20. Low Density Supersonic Decelerator (LDSD) Supersonic Flight Dynamics Test (SFDT) Plume Induced Environment Modelling

    Science.gov (United States)

    Mobley, B. L.; Smith, S. D.; Van Norman, J. W.; Muppidi, S.; Clark, I

    2016-01-01

    Provide plume induced heating (radiation & convection) predictions in support of the LDSD thermal design (pre-flight SFDT-1) Predict plume induced aerodynamics in support of flight dynamics, to achieve targeted freestream conditions to test supersonic deceleration technologies (post-flight SFDT-1, pre-flight SFDT-2)

  1. NASA/FAA Tailplane Icing Program: Flight Test Report

    Science.gov (United States)

    Ratvasky, Thomas P.; VanZante, Judith Foss; Sim, Alex

    2000-01-01

    This report presents results from research flights that explored the characteristics of an ice-contaminated tailplane using various simulated ice shapes attached to the leading edge of the horizontal tailplane. A clean leading edge provided the baseline case, then three ice shapes were flown in order of increasing severity. Flight tests included both steady state and dynamic maneuvers. The steady state points were 1G wings level and steady heading sideslips. The primary dynamic maneuvers were pushovers to various G-levels; elevator doublets; and thrust transitions. These maneuvers were conducted for a full range of flap positions and aircraft angle of attack where possible. The analysis of this data set has clearly demonstrated the detrimental effects of ice contamination on aircraft stability and controllability. Paths to tailplane stall were revealed through parameter isolation and transition studies. These paths are (1) increasing ice shape severity, (2) increasing flap deflection, (3) high or low speeds, depending on whether the aircraft is in a steady state (high speed) or pushover maneuver (low speed), and (4) increasing thrust. The flight research effort was very comprehensive, but did not examine effects of tailplane design and location, or other aircraft geometry configuration effects. However, this effort provided the role of some of the parameters in promoting tailplane stall. The lessons learned will provide guidance to regulatory agencies, aircraft manufacturers, and operators on ice-contaminated tailplane stall in the effort to increase aviation safety and reduce the fatal accident rate.

  2. Airborne Turbulence Detection and Warning ACLAIM Flight Test Results

    Science.gov (United States)

    Hannon, Stephen M.; Bagley, Hal R.; Soreide, Dave C.; Bowdle, David A.; Bogue, Rodney K.; Ehernberger, L. Jack

    1999-01-01

    The Airborne Coherent Lidar for Advanced Inflight Measurements (ACLAIM) is a NASA/Dryden-lead program to develop and demonstrate a 2 micrometers pulsed Doppler lidar for airborne look-ahead turbulence detection and warning. Advanced warning of approaching turbulence can significantly reduce injuries to passengers and crew aboard commercial airliners. The ACLAIM instrument is a key asset to the ongoing Turbulence component of NASA's Aviation Safety Program, aimed at reducing the accident rate aboard commercial airliners by a factor of five over the next ten years and by a factor of ten over the next twenty years. As well, the advanced turbulence warning capability can prevent "unstarts" in the inlet of supersonic aircraft engines by alerting the flight control computer which then adjusts the engine to operate in a less fuel efficient, and more turbulence tolerant, mode. Initial flight tests of the ACLAIM were completed in March and April of 1998. This paper and presentation gives results from these initial flights, with validated demonstration of Doppler lidar wind turbulence detection several kilometers ahead of the aircraft.

  3. Development and flight testing of UV optimized Photon Counting CCDs

    Science.gov (United States)

    Hamden, Erika T.

    2018-06-01

    I will discuss the latest results from the Hamden UV/Vis Detector Lab and our ongoing work using a UV optimized EMCCD in flight. Our lab is currently testing efficiency and performance of delta-doped, anti-reflection coated EMCCDs, in collaboration with JPL. The lab has been set-up to test quantum efficiency, dark current, clock-induced-charge, and read noise. I will describe our improvements to our circuit boards for lower noise, updates from a new, more flexible NUVU controller, and the integration of an EMCCD in the FIREBall-2 UV spectrograph. I will also briefly describe future plans to conduct radiation testing on delta-doped EMCCDs (both warm, unbiased and cold, biased configurations) thus summer and longer term plans for testing newer photon counting CCDs as I move the HUVD Lab to the University of Arizona in the Fall of 2018.

  4. Preliminary flight test results of a fly-by-throttle emergency flight control system on an F-15 airplane

    Science.gov (United States)

    Burcham, Frank W., Jr.; Maine, Trindel A.; Fullerton, C. G.; Wells, Edward A.

    1993-01-01

    A multi-engine aircraft, with some or all of the flight control system inoperative, may use engine thrust for control. NASA Dryden has conducted a study of the capability and techniques for this emergency flight control method for the F-15 airplane. With an augmented control system, engine thrust, along with appropriate feedback parameters, is used to control flightpath and bank angle. Extensive simulation studies have been followed by flight tests. This paper discusses the principles of throttles-only control, the F-15 airplane, the augmented system, and the flight results including landing approaches with throttles-only control to within 10 ft of the ground.

  5. Kodak Mirror Assembly Tested at Marshall Space Flight Center

    Science.gov (United States)

    2003-01-01

    This photo (a frontal view) is of one of many segments of the Eastman-Kodak mirror assembly being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

  6. Tests and calibration of NIF neutron time of flight detectors.

    Science.gov (United States)

    Ali, Z A; Glebov, V Yu; Cruz, M; Duffy, T; Stoeckl, C; Roberts, S; Sangster, T C; Tommasini, R; Throop, A; Moran, M; Dauffy, L; Horsefield, C

    2008-10-01

    The National Ignition Facility (NIF) neutron time of flight (NTOF) diagnostic will measure neutron yield and ion temperature in all NIF campaigns in DD, DT, and THD(*) implosions. The NIF NTOF diagnostic is designed to measure neutron yield from 1x10(9) to 2x10(19). The NTOF consists of several detectors of varying sensitivity located on the NIF at about 5 and 20 m from the target. Production, testing, and calibration of the NIF NTOF detectors have begun at the Laboratory for Laser Energetics (LLE). Operational tests of the NTOF detectors were performed on several facilities including the OMEGA laser at LLE and the Titan laser at Lawrence Livermore National Laboratory. Neutron calibrations were carried out on the OMEGA laser. Results of the NTOF detector tests and calibration will be presented.

  7. Flight tests of a supersonic natural laminar flow airfoil

    International Nuclear Information System (INIS)

    Frederick, M A; Banks, D W; Garzon, G A; Matisheck, J R

    2015-01-01

    A flight test campaign of a supersonic natural laminar flow airfoil has been recently completed. The test surface was an 80 inch (203 cm) chord and 40 inch (102 cm) span article mounted on the centerline store location of an F-15B airplane. The test article was designed with a leading edge sweep of effectively 0° to minimize boundary layer crossflow. The test article surface was coated with an insulating material to avoid significant heat transfer to and from the test article structure to maintain a quasi-adiabatic wall. An aircraft-mounted infrared camera system was used to determine boundary layer transition and the extent of laminar flow. The tests were flown up to Mach 2.0 and chord Reynolds numbers in excess of 30 million. The objectives of the tests were to determine the extent of laminar flow at high Reynolds numbers and to determine the sensitivity of the flow to disturbances. Both discrete (trip dots) and 2D disturbances (forward-facing steps) were tested. A series of oblique shocks, of yet unknown origin, appeared on the surface, which generated sufficient crossflow to affect transition. Despite the unwanted crossflow, the airfoil performed well. The results indicate that the sensitivity of the flow to the disturbances, which can translate into manufacturing tolerances, was similar to that of subsonic natural laminar flow wings. (paper)

  8. PINS Testing and Modification for Explosive Identification

    International Nuclear Information System (INIS)

    Seabury, E.H.; Caffrey, A.J.

    2011-01-01

    The INL's Portable Isotopic Neutron Spectroscopy System (PINS)1 non-intrusively identifies the chemical fill of munitions and sealed containers. PINS is used routinely by the U.S. Army, the Defense Threat Reduction Agency, and foreign military units to determine the contents of munitions and other containers suspected to contain explosives, smoke-generating chemicals, and chemical warfare agents such as mustard and nerve gas. The objects assayed with PINS range from softball-sized M139 chemical bomblets to 200 gallon DOT 500X ton containers. INL had previously examined2 the feasibility of using a similar system for the identification of explosives, and based on this proof-of-principle test, the development of a dedicated system for the identification of explosives in an improvised nuclear device appears entirely feasible. INL has been tasked by NNSA NA-42 Render Safe Research and Development with the development of such a system.

  9. Pre-Flight Ground Testing of the Full-Scale HIFiRE-1 at Fully Duplicated Flight Conditions

    National Research Council Canada - National Science Library

    Wadhams, Tim P; MacLean, Matthew G; Holden, Michael S; Mundy, Erik

    2008-01-01

    As part of an experimental study to obtain detailed heating and pressure data over the full-scale HIFiRE-1 flight geometry, CUBRC has completed a 30-run matrix of ground tests, sponsored by the AFOSR...

  10. Digital virtual flight testing and evaluation method for flight characteristics airworthiness compliance of civil aircraft based on HQRM

    Directory of Open Access Journals (Sweden)

    Fan Liu

    2015-02-01

    Full Text Available In order to incorporate airworthiness requirements for flight characteristics into the entire development cycle of electronic flight control system (EFCS equipped civil aircraft, digital virtual flight testing and evaluation method based on handling qualities rating method (HQRM is proposed. First, according to HQRM, flight characteristics airworthiness requirements of civil aircraft in EFCS failure states are determined. On this basis, digital virtual flight testing model, comprising flight task digitized model, pilot controlling model, aircraft motion and atmospheric turbulence model, is used to simulate the realistic process of a pilot controlling an airplane to perform assigned flight tasks. According to the simulation results, flight characteristics airworthiness compliance of the airplane can be evaluated relying on the relevant regulations for handling qualities (HQ rating. Finally, this method is applied to a type of passenger airplane in a typical EFCS failure state, and preliminary conclusions concerning airworthiness compliance are derived quickly. The research results of this manuscript can provide important theoretical reference for EFCS design and actual airworthiness compliance verification of civil aircraft.

  11. Flight Test Results of a GPS-Based Pitot-Static Calibration Method Using Output-Error Optimization for a Light Twin-Engine Airplane

    Science.gov (United States)

    Martos, Borja; Kiszely, Paul; Foster, John V.

    2011-01-01

    As part of the NASA Aviation Safety Program (AvSP), a novel pitot-static calibration method was developed to allow rapid in-flight calibration for subscale aircraft while flying within confined test areas. This approach uses Global Positioning System (GPS) technology coupled with modern system identification methods that rapidly computes optimal pressure error models over a range of airspeed with defined confidence bounds. This method has been demonstrated in subscale flight tests and has shown small 2- error bounds with significant reduction in test time compared to other methods. The current research was motivated by the desire to further evaluate and develop this method for full-scale aircraft. A goal of this research was to develop an accurate calibration method that enables reductions in test equipment and flight time, thus reducing costs. The approach involved analysis of data acquisition requirements, development of efficient flight patterns, and analysis of pressure error models based on system identification methods. Flight tests were conducted at The University of Tennessee Space Institute (UTSI) utilizing an instrumented Piper Navajo research aircraft. In addition, the UTSI engineering flight simulator was used to investigate test maneuver requirements and handling qualities issues associated with this technique. This paper provides a summary of piloted simulation and flight test results that illustrates the performance and capabilities of the NASA calibration method. Discussion of maneuver requirements and data analysis methods is included as well as recommendations for piloting technique.

  12. Overheating Anomalies during Flight Test Due to the Base Bleeding

    Science.gov (United States)

    Luchinsky, Dmitry; Hafiychuck, Halyna; Osipov, Slava; Ponizhovskaya, Ekaterina; Smelyanskiy, Vadim; Dagostino, Mark; Canabal, Francisco; Mobley, Brandon L.

    2012-01-01

    In this paper we present the results of the analytical and numerical studies of the plume interaction with the base flow in the presence of base out-gassing. The physics-based analysis and CFD modeling of the base heating for single solid rocket motor performed in this research addressed the following questions: what are the key factors making base flow so different from that in the Shuttle [1]; why CFD analysis of this problem reveals small plume recirculation; what major factors influence base temperature; and why overheating was initiated at a given time in the flight. To answer these questions topological analysis of the base flow was performed and Korst theory was used to estimate relative contributions of radiation, plume recirculation, and chemically reactive out-gassing to the base heating. It was shown that base bleeding and small base volume are the key factors contributing to the overheating, while plume recirculation is effectively suppressed by asymmetric configuration of the flow formed earlier in the flight. These findings are further verified using CFD simulations that include multi-species gas environment both in the plume and in the base. Solid particles in the exhaust plume (Al2O3) and char particles in the base bleeding were also included into the simulations and their relative contributions into the base temperature rise were estimated. The results of simulations are in good agreement with the temperature and pressure in the base measured during the test.

  13. Technical testing of portable isotope identification instruments

    International Nuclear Information System (INIS)

    Smith, D.E.

    2001-01-01

    . First generation portable isotope identifiers were identified and evaluated by U.S. Customs. Instruments from five vendors were put through a battery of tests according to delineated test procedures. The tests fell into five categories: Basic operation verification; Medical isotope identification; Individual isotope identification; Isotope pair identification; Dose rate calculation. The tests can be summarized as an initial verification of correct instrument operation (including whatever calibration procedure was specified by the vendor), followed by three different isotope identification tests designed to verify the instrument's ability to correctly identify 19 isotopes specified by U.S. Customs. The final test determined each instrument's ability to correctly display the dose rate from a wide energy range of gamma emitters. The individual isotope identification test and the isotope pair identification test were performed with three types of intervening shielding. The first of these three types used the minimum packaging needed for safe source handling and was, in general, thin plastic. This type of shielding is virtually identical to a bare radiation source. The second type of shielding used 2.7 mm of steel to simulate the wall of a typical transportainer (or sea-tainer). The third type of shielding used 6.94 mm of lead to simulate typical industrial packaging or, perhaps, an attempt by the shipper to conceal the source without unduly increasing the weight of the shipment. The isotopes tested were broadly categorized as: Special nuclear material (SNM), Medical, and Industrial. These included 17 gamma emitters, 1 beta emitter, and 1 neutron emitter. Each instrument was subjected to the following testing regimen: First, each instrument was calibrated according to vendor's procedures. Instrument size and weight were recorded. Next, the instrument's response to medical isotopes in their usual shipping containers was measured. This represents the most commonly occurring

  14. Design and Testing of Flight Control Laws on the RASCAL Research Helicopter

    Science.gov (United States)

    Frost, Chad R.; Hindson, William S.; Moralez. Ernesto, III; Tucker, George E.; Dryfoos, James B.

    2001-01-01

    Two unique sets of flight control laws were designed, tested and flown on the Army/NASA Rotorcraft Aircrew Systems Concepts Airborne Laboratory (RASCAL) JUH-60A Black Hawk helicopter. The first set of control laws used a simple rate feedback scheme, intended to facilitate the first flight and subsequent flight qualification of the RASCAL research flight control system. The second set of control laws comprised a more sophisticated model-following architecture. Both sets of flight control laws were developed and tested extensively using desktop-to-flight modeling, analysis, and simulation tools. Flight test data matched the model predicted responses well, providing both evidence and confidence that future flight control development for RASCAL will be efficient and accurate.

  15. Orion Exploration Flight Test-1 Post-Flight Navigation Performance Assessment Relative to the Best Estimated Trajectory

    Science.gov (United States)

    Gay, Robert S.; Holt, Greg N.; Zanetti, Renato

    2016-01-01

    This paper details the post-flight navigation performance assessment of the Orion Exploration Flight Test-1 (EFT-1). Results of each flight phase are presented: Ground Align, Ascent, Orbit, and Entry Descent and Landing. This study examines the on-board Kalman Filter uncertainty along with state deviations relative to the Best Estimated Trajectory (BET). Overall the results show that the Orion Navigation System performed as well or better than expected. Specifically, the Global Positioning System (GPS) measurement availability was significantly better than anticipated at high altitudes. In addition, attitude estimation via processing GPS measurements along with Inertial Measurement Unit (IMU) data performed very well and maintained good attitude throughout the mission.

  16. Perseus B Taxi Tests in Preparation for a New Series of Flight Tests

    Science.gov (United States)

    1998-01-01

    The Perseus B remotely piloted aircraft taxis on the runway at Edwards Air Force Base, California, before a series of development flights at NASA's Dryden flight Research Center. The Perseus B is the latest of three versions of the Perseus design developed by Aurora Flight Sciences under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program. Perseus B is a remotely piloted aircraft developed as a design-performance testbed under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) project. Perseus is one of several flight vehicles involved in the ERAST project. A piston engine, propeller-powered aircraft, Perseus was designed and built by Aurora Flight Sciences Corporation, Manassas, Virginia. The objectives of Perseus B's ERAST flight tests have been to reach and maintain horizontal flight above altitudes of 60,000 feet and demonstrate the capability to fly missions lasting from 8 to 24 hours, depending on payload and altitude requirements. The Perseus B aircraft established an unofficial altitude record for a single-engine, propeller-driven, remotely piloted aircraft on June 27, 1998. It reached an altitude of 60,280 feet. In 1999, several modifications were made to the Perseus aircraft including engine, avionics, and flight-control-system improvements. These improvements were evaluated in a series of operational readiness and test missions at the Dryden Flight Research Center, Edwards, California. Perseus is a high-wing monoplane with a conventional tail design. Its narrow, straight, high-aspect-ratio wing is mounted atop the fuselage. The aircraft is pusher-designed with the propeller mounted in the rear. This design allows for interchangeable scientific-instrument payloads to be placed in the forward fuselage. The design also allows for unobstructed airflow to the sensors and other devices mounted in the payload compartment. The Perseus B that underwent test and development in 1999 was the third generation of the Perseus

  17. Fault Tolerant Flight Control Using Sliding Modes and Subspace Identification-Based Predictive Control

    KAUST Repository

    Siddiqui, Bilal A.; El-Ferik, Sami; Abdelkader, Mohamed

    2016-01-01

    In this work, a cascade structure of a time-scale separated integral sliding mode and model predictive control is proposed as a viable alternative for fault-tolerant control. A multi-variable sliding mode control law is designed as the inner loop of the flight control system. Subspace identification is carried out on the aircraft in closed loop. The identified plant is then used for model predictive controllers in the outer loop. The overall control law demonstrates improved robustness to measurement noise, modeling uncertainties, multiple faults and severe wind turbulence and gusts. In addition, the flight control system employs filters and dead-zone nonlinear elements to reduce chattering and improve handling quality. Simulation results demonstrate the efficiency of the proposed controller using conventional fighter aircraft without control redundancy.

  18. Fault Tolerant Flight Control Using Sliding Modes and Subspace Identification-Based Predictive Control

    KAUST Repository

    Siddiqui, Bilal A.

    2016-07-26

    In this work, a cascade structure of a time-scale separated integral sliding mode and model predictive control is proposed as a viable alternative for fault-tolerant control. A multi-variable sliding mode control law is designed as the inner loop of the flight control system. Subspace identification is carried out on the aircraft in closed loop. The identified plant is then used for model predictive controllers in the outer loop. The overall control law demonstrates improved robustness to measurement noise, modeling uncertainties, multiple faults and severe wind turbulence and gusts. In addition, the flight control system employs filters and dead-zone nonlinear elements to reduce chattering and improve handling quality. Simulation results demonstrate the efficiency of the proposed controller using conventional fighter aircraft without control redundancy.

  19. The SCS double hydrometer test in dispersive soil identification

    CSIR Research Space (South Africa)

    Maharaj, A

    2013-09-01

    Full Text Available The standard testing procedures for the Soil Conservation Service (SCS) Double Hydrometer test, the Pinhole Test, Crumb test and chemical analyses for the identification of potentially dispersive soils have recently been studied and problems...

  20. Aeroelastic model identification of winglet loads from flight test data

    OpenAIRE

    Reijerkerk, M.J.

    2008-01-01

    Numerical computational methods are getting more and more sophisticated every day, enabling more accurate aircraft load predictions. In the structural design of aircraft higher levels of flexibility can be tolerated to arrive at a substantial weight reduction. The result is that aircraft of the future can be bigger, have better performance and less mass. The performance of an aircraft can be even further enhanced by the use of winglets or other wing tip devices. A more flexible structure in c...

  1. Aeroelastic model identification of winglet loads from flight test data

    NARCIS (Netherlands)

    Reijerkerk, M.J.

    2008-01-01

    Numerical computational methods are getting more and more sophisticated every day, enabling more accurate aircraft load predictions. In the structural design of aircraft higher levels of flexibility can be tolerated to arrive at a substantial weight reduction. The result is that aircraft of the

  2. Aerodynamic Flight-Test Results for the Adaptive Compliant Trailing Edge

    Science.gov (United States)

    Cumming, Stephen B.; Smith, Mark S.; Ali, Aliyah N.; Bui, Trong T.; Ellsworth, Joel C.; Garcia, Christian A.

    2016-01-01

    The aerodynamic effects of compliant flaps installed onto a modified Gulfstream III airplane were investigated. Analyses were performed prior to flight to predict the aerodynamic effects of the flap installation. Flight tests were conducted to gather both structural and aerodynamic data. The airplane was instrumented to collect vehicle aerodynamic data and wing pressure data. A leading-edge stagnation detection system was also installed. The data from these flights were analyzed and compared with predictions. The predictive tools compared well with flight data for small flap deflections, but differences between predictions and flight estimates were greater at larger deflections. This paper describes the methods used to examine the aerodynamics data from the flight tests and provides a discussion of the flight-test results in the areas of vehicle aerodynamics, wing sectional pressure coefficient profiles, and air data.

  3. The Cryogenic Test Bed experiments: Cryogenic heat pipe flight experiment CRYOHP (STS-53). Cryogenic two phase flight experiment CRYOTP (STS-62). Cryogenic flexible diode flight experiment CRYOFD

    Science.gov (United States)

    Thienel, Lee; Stouffer, Chuck

    1995-09-01

    This paper presents an overview of the Cryogenic Test Bed (CTB) experiments including experiment results, integration techniques used, and lessons learned during integration, test and flight phases of the Cryogenic Heat Pipe Flight Experiment (STS-53) and the Cryogenic Two Phase Flight Experiment (OAST-2, STS-62). We will also discuss the Cryogenic Flexible Diode Heat Pipe (CRYOFD) experiment which will fly in the 1996/97 time frame and the fourth flight of the CTB which will fly in the 1997/98 time frame. The two missions tested two oxygen axially grooved heat pipes, a nitrogen fibrous wick heat pipe and a 2-methylpentane phase change material thermal storage unit. Techniques were found for solving problems with vibration from the cryo-collers transmitted through the compressors and the cold heads, and mounting the heat pipe without introducing parasitic heat leaks. A thermally conductive interface material was selected that would meet the requirements and perform over the temperature range of 55 to 300 K. Problems are discussed with the bi-metallic thermostats used for heater circuit protection and the S-Glass suspension straps originally used to secure the BETSU PCM in the CRYOTP mission. Flight results will be compared to 1-g test results and differences will be discussed.

  4. MATE: Modern Software Technology for Flight Test Automation and Orchestration, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — The development of advanced technologies for flight testing, measurement, and data acquisition are critical to effectively meeting the future goals and challenges...

  5. Command and Data Handling Flight Software test framework: A Radiation Belt Storm Probes practice

    Science.gov (United States)

    Hill, T. A.; Reid, W. M.; Wortman, K. A.

    During the Radiation Belt Storm Probes (RBSP) mission, a test framework was developed by the Embedded Applications Group in the Space Department at the Johns Hopkins Applied Physics Laboratory (APL). The test framework is implemented for verification of the Command and Data Handling (C& DH) Flight Software. The RBSP C& DH Flight Software consists of applications developed for use with Goddard Space Flight Center's core Flight Executive (cFE) architecture. The test framework's initial concept originated with tests developed for verification of the Autonomy rules that execute with the Autonomy Engine application of the RBSP C& DH Flight Software. The test framework was adopted and expanded for system and requirements verification of the RBSP C& DH Flight Software. During the evolution of the RBSP C& DH Flight Software test framework design, a set of script conventions and a script library were developed. The script conventions and library eased integration of system and requirements verification tests into a comprehensive automated test suite. The comprehensive test suite is currently being used to verify releases of the RBSP C& DH Flight Software. In addition to providing the details and benefits of the test framework, the discussion will include several lessons learned throughout the verification process of RBSP C& DH Flight Software. Our next mission, Solar Probe Plus (SPP), will use the cFE architecture for the C& DH Flight Software. SPP also plans to use the same ground system as RBSP. Many of the RBSP C& DH Flight Software applications are reusable on the SPP mission, therefore there is potential for test design and test framework reuse for system and requirements verification.

  6. Hyper-X Mach 7 Scramjet Design, Ground Test and Flight Results

    Science.gov (United States)

    Ferlemann, Shelly M.; McClinton, Charles R.; Rock, Ken E.; Voland, Randy T.

    2005-01-01

    The successful Mach 7 flight test of the Hyper-X (X-43) research vehicle has provided the major, essential demonstration of the capability of the airframe integrated scramjet engine. This flight was a crucial first step toward realizing the potential for airbreathing hypersonic propulsion for application to space launch vehicles. However, it is not sufficient to have just achieved a successful flight. The more useful knowledge gained from the flight is how well the prediction methods matched the actual test results in order to have confidence that these methods can be applied to the design of other scramjet engines and powered vehicles. The propulsion predictions for the Mach 7 flight test were calculated using the computer code, SRGULL, with input from computational fluid dynamics (CFD) and wind tunnel tests. This paper will discuss the evolution of the Mach 7 Hyper-X engine, ground wind tunnel experiments, propulsion prediction methodology, flight results and validation of design methods.

  7. Use of matrix assisted laser desorption ionisation-time of flight mass spectrometry in a paediatric clinical laboratory for identification of bacteria commonly isolated from cystic fibrosis patients.

    Science.gov (United States)

    Desai, Ankita Patel; Stanley, Theresa; Atuan, Maria; McKey, Jonelle; Lipuma, John J; Rogers, Beverly; Jerris, Robert

    2012-09-01

    Matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) has been described as a rapid, accurate method for bacterial identification. To investigate the ability of the technique, using the unamended database supplied with the system, to identify bacteria commonly isolated in cystic fibrosis (CF) patients. Organisms commonly isolated from CF patients identified by MALDI-TOF MS were compared to conventional phenotypic and genotypic analyses. For MALDI-TOF MS, the direct colony technique was used routinely with one extraction procedure performed on a mucoid Pseudomonas aeruginosa. For 24 unique CF specimens, workload comparison and time to identification were assessed. Of 464 tested isolates, conventional (phenotypic and genotypic) identification compared to MALDI-TOF MS showed complete genus, species agreement in 92%, with genus agreement in 98%. This included 29 isolates within the Burkholderia cepacia complex. All 29 were correctly identified to the genus level and 24 of these were speciated. Time to identification with 47 bacterial isolates from 24 CF patients showed identification of 85% of isolates by MALDI-TOF MS at 48 h of incubation, compared to only 34% with conventional methods. Using the unamended database supplied with the system, MALDI-TOF MS provides rapid and reliable identification of bacteria isolated from CF specimens. Time to identification studies showed that the use of same day, same method for organism identification will decrease time to result and optimise microbiology workflow.

  8. NASA Langley's AirSTAR Testbed: A Subscale Flight Test Capability for Flight Dynamics and Control System Experiments

    Science.gov (United States)

    Jordan, Thomas L.; Bailey, Roger M.

    2008-01-01

    As part of the Airborne Subscale Transport Aircraft Research (AirSTAR) project, NASA Langley Research Center (LaRC) has developed a subscaled flying testbed in order to conduct research experiments in support of the goals of NASA s Aviation Safety Program. This research capability consists of three distinct components. The first of these is the research aircraft, of which there are several in the AirSTAR stable. These aircraft range from a dynamically-scaled, twin turbine vehicle to a propeller driven, off-the-shelf airframe. Each of these airframes carves out its own niche in the research test program. All of the airplanes have sophisticated on-board data acquisition and actuation systems, recording, telemetering, processing, and/or receiving data from research control systems. The second piece of the testbed is the ground facilities, which encompass the hardware and software infrastructure necessary to provide comprehensive support services for conducting flight research using the subscale aircraft, including: subsystem development, integrated testing, remote piloting of the subscale aircraft, telemetry processing, experimental flight control law implementation and evaluation, flight simulation, data recording/archiving, and communications. The ground facilities are comprised of two major components: (1) The Base Research Station (BRS), a LaRC laboratory facility for system development, testing and data analysis, and (2) The Mobile Operations Station (MOS), a self-contained, motorized vehicle serving as a mobile research command/operations center, functionally equivalent to the BRS, capable of deployment to remote sites for supporting flight tests. The third piece of the testbed is the test facility itself. Research flights carried out by the AirSTAR team are conducted at NASA Wallops Flight Facility (WFF) on the Eastern Shore of Virginia. The UAV Island runway is a 50 x 1500 paved runway that lies within restricted airspace at Wallops Flight Facility. The

  9. CFD to Flight: Some Recent Success Stories of X-Plane Design to Flight Test at the NASA Dryden Flight Research Center

    Science.gov (United States)

    Cosentino, Gary B.

    2007-01-01

    Several examples from the past decade of success stories involving the design and flight test of three true X-planes will be described: in particular, X-plane design techniques that relied heavily upon computational fluid dynamics (CFD). Three specific examples chosen from the author s personal experience are presented: the X-36 Tailless Fighter Agility Research Aircraft, the X-45A Unmanned Combat Air Vehicle, and, most recently, the X-48B Blended Wing Body Demonstrator Aircraft. An overview will be presented of the uses of CFD analysis, comparisons and contrasts with wind tunnel testing, and information derived from the CFD analysis that directly related to successful flight test. Some lessons learned on the proper application, and misapplication, of CFD are illustrated. Finally, some highlights of the flight-test results of the three example X-planes will be presented. This overview paper will discuss some of the author s experience with taking an aircraft shape from early concept and three-dimensional modeling through CFD analysis, wind tunnel testing, further refined CFD analysis, and, finally, flight. An overview of the key roles in which CFD plays well during this process, and some other roles in which it does not, are discussed. How wind tunnel testing complements, calibrates, and verifies CFD analysis is also covered. Lessons learned on where CFD results can be misleading are also given. Strengths and weaknesses of the various types of flow solvers, including panel methods, Euler, and Navier-Stokes techniques, are discussed. The paper concludes with the three specific examples, including some flight test video footage of the X-36, the X-45A, and the X-48B.

  10. Design of the TORCH detector: A Cherenkov based Time-of-Flight system for particle identification

    CERN Document Server

    AUTHOR|(CDS)2078663; Rademacker, Jonas

    The LHCb detector at the LHC collider has been very successfully operated over the past years, providing new and profound insights into the Standard Model, in particular through study of $b$-hadrons to achieve a better understanding of CP violation. One of the key components of LHCb is its particle identification system, comprised of two RICH detectors, which allow for high precision separation of particle species over a large momentum range. In order to retain and improve the performance of the particle identification system in light of the LHCb upgrade, the TORCH detector has been proposed to supplement the RICH system at low momentum (2-10 GeV/c). The TORCH detector provides (charged) particle identification through precision timing of particles passing through it. Assuming a known momentum from the tracking, it is possible to derive the species of a particle from the time of flight from its primary vertex. This measurement is achieved by timing and combining photons generated in a solid radiator. The geom...

  11. The X-43A Hyper-X Mach 7 Flight 2 Guidance, Navigation, and Control Overview and Flight Test Results

    Science.gov (United States)

    Bahm, Catherine; Baumann, Ethan; Martin, John; Bose, David; Beck, Roger E.; Strovers, Brian

    2005-01-01

    The objective of the Hyper-X program was to flight demonstrate an airframe-integrated hypersonic vehicle. On March 27, 2004, the Hyper-X program team successfully conducted flight 2 and achieved all of the research objectives. The Hyper-X research vehicle successfully separated from the Hyper-X launch vehicle and achieved the desired engine test conditions before the experiment began. The research vehicle rejected the disturbances caused by the cowl door opening and the fuel turning on and off and maintained the engine test conditions throughout the experiment. After the engine test was complete, the vehicle recovered and descended along a trajectory while performing research maneuvers. The last data acquired showed that the vehicle maintained control to the water. This report will provide an overview of the research vehicle guidance and control systems and the performance of the vehicle during the separation event and engine test. The research maneuvers were performed to collect data for aerodynamics and flight controls research. This report also will provide an overview of the flight controls related research and results.

  12. Numerical CFD Simulation and Test Correlation in a Flight Project Environment

    Science.gov (United States)

    Gupta, K. K.; Lung, S. F.; Ibrahim, A. H.

    2015-01-01

    This paper presents detailed description of a novel CFD procedure and comparison of its solution results to that obtained by other available CFD codes as well as actual flight and wind tunnel test data pertaining to the GIII aircraft, currently undergoing flight testing at AFRC.

  13. Advanced fighter technology integration (AFTI)/F-16 Automated Maneuvering Attack System final flight test results

    Science.gov (United States)

    Dowden, Donald J.; Bessette, Denis E.

    1987-01-01

    The AFTI F-16 Automated Maneuvering Attack System has undergone developmental and demonstration flight testing over a total of 347.3 flying hours in 237 sorties. The emphasis of this phase of the flight test program was on the development of automated guidance and control systems for air-to-air and air-to-ground weapons delivery, using a digital flight control system, dual avionics multiplex buses, an advanced FLIR sensor with laser ranger, integrated flight/fire-control software, advanced cockpit display and controls, and modified core Multinational Stage Improvement Program avionics.

  14. A new method for flight test determination of propulsive efficiency and drag coefficient

    Science.gov (United States)

    Bull, G.; Bridges, P. D.

    1983-01-01

    A flight test method is described from which propulsive efficiency as well as parasite and induced drag coefficients can be directly determined using relatively simple instrumentation and analysis techniques. The method uses information contained in the transient response in airspeed for a small power change in level flight in addition to the usual measurement of power required for level flight. Measurements of pitch angle and longitudinal and normal acceleration are eliminated. The theoretical basis for the method, the analytical techniques used, and the results of application of the method to flight test data are presented.

  15. Post Flight Analysis Of SHEFEX I: Shock Tunnel Testing And Related CFD Analysis

    Science.gov (United States)

    Schramm, Jan Martinez; Barth, Tarik; Wagner, Alexander; Hannemann, Klaus

    2011-05-01

    The SHarp Edge Flight EXperiment (SHEFEX) program of the German Aerospace Center (DLR) is primarily focused on the investigation of the potential to utilise improved shapes for space vehicles by considering sharp edges and facetted surfaces. One goal is to set up a sky based test facility to gain knowledge of the physics of hypersonic flow, complemented by numerical analysis and ground based testing. Further, the series of SHEFEX flight experiments is an excellent test bed for new technological concepts and flight instrumentation, and it is a source of motivation for young scientist and engineers providing an excellent school for future space-program engineers and managers. After the successful first SHEFEX flight in October 2005, a second flight is scheduled for September 2011 and additional flights are planned for 2015 ff. With the SHEFEX-I flight and the subsequent numerical and experimental post flight analysis, DLR could for the first time close the loop between the three major disciplines of aerothermodynamic research namely CFD, ground based testing and flight.

  16. Multi-Sensor Testing for Automated Rendezvous and Docking Sensor Testing at the Flight Robotics Laboratory

    Science.gov (United States)

    Brewster, L.; Johnston, A.; Howard, R.; Mitchell, J.; Cryan, S.

    2007-01-01

    The Exploration Systems Architecture defines missions that require rendezvous, proximity operations, and docking (RPOD) of two spacecraft both in Low Earth Orbit (LEO) and in Low Lunar Orbit (LLO). Uncrewed spacecraft must perform automated and/or autonomous rendezvous, proximity operations and docking operations (commonly known as AR&D). The crewed missions may also perform rendezvous and docking operations and may require different levels of automation and/or autonomy, and must provide the crew with relative navigation information for manual piloting. The capabilities of the RPOD sensors are critical to the success of the Exploration Program. NASA has the responsibility to determine whether the Crew Exploration Vehicle (CEV) contractor proposed relative navigation sensor suite will meet the requirements. The relatively low technology readiness level of AR&D relative navigation sensors has been carried as one of the CEV Project's top risks. The AR&D Sensor Technology Project seeks to reduce the risk by the testing and analysis of selected relative navigation sensor technologies through hardware-in-the-loop testing and simulation. These activities will provide the CEV Project information to assess the relative navigation sensors maturity as well as demonstrate test methods and capabilities. The first year of this project focused on a series of"pathfinder" testing tasks to develop the test plans, test facility requirements, trajectories, math model architecture, simulation platform, and processes that will be used to evaluate the Contractor-proposed sensors. Four candidate sensors were used in the first phase of the testing. The second phase of testing used four sensors simultaneously: two Marshall Space Flight Center (MSFC) Advanced Video Guidance Sensors (AVGS), a laser-based video sensor that uses retroreflectors attached to the target vehicle, and two commercial laser range finders. The multi-sensor testing was conducted at MSFC's Flight Robotics Laboratory (FRL

  17. Multi-Sensor Testing for Automated Rendezvous and Docking Sensor Testing at the Flight Robotics Lab

    Science.gov (United States)

    Brewster, Linda L.; Howard, Richard T.; Johnston, A. S.; Carrington, Connie; Mitchell, Jennifer D.; Cryan, Scott P.

    2008-01-01

    The Exploration Systems Architecture defines missions that require rendezvous, proximity operations, and docking (RPOD) of two spacecraft both in Low Earth Orbit (LEO) and in Low Lunar Orbit (LLO). Uncrewed spacecraft must perform automated and/or autonomous rendezvous, proximity operations and docking operations (commonly known as AR&D). The crewed missions may also perform rendezvous and docking operations and may require different levels of automation and/or autonomy, and must provide the crew with relative navigation information for manual piloting. The capabilities of the RPOD sensors are critical to the success ofthe Exploration Program. NASA has the responsibility to determine whether the Crew Exploration Vehicle (CEV) contractor-proposed relative navigation sensor suite will meet the requirements. The relatively low technology readiness level of AR&D relative navigation sensors has been carried as one of the CEV Project's top risks. The AR&D Sensor Technology Project seeks to reduce the risk by the testing and analysis of selected relative navigation sensor technologies through hardware-in-the-Ioop testing and simulation. These activities will provide the CEV Project information to assess the relative navigation sensors maturity as well as demonstrate test methods and capabilities. The first year of this project focused on a series of "pathfinder" testing tasks to develop the test plans, test facility requirements, trajectories, math model architecture, simulation platform, and processes that will be used to evaluate the Contractor-proposed sensors. Four candidate sensors were used in the first phase of the testing. The second phase of testing used four sensors simultaneously: two Marshall Space Flight Center (MSFC) Advanced Video Guidance Sensors (AVGS), a laser-based video sensor that uses retroreflectors attached to the target vehicle, and two commercial laser range finders. The multi-sensor testing was conducted at MSFC's Flight Robotics Laboratory (FRL

  18. Mission definition study for Stanford relativity satellite. Volume 2: Engineering flight test program

    Science.gov (United States)

    1971-01-01

    The need is examined for orbital flight tests of gyroscope, dewar, and other components, in order to reduce the technical and financial risk in performing the relativity experiment. A program is described that would generate engineering data to permit prediction of final performance. Two flight tests are recommended. The first flight would test a dewar smaller than that required for the final flight, but of size and form sufficient to allow extrapolation to the final design. The second flight would use the same dewar design to carry a set of three gyroscopes, which would be evaluated for spinup and drift characteristics for a period of a month or more. A proportional gas control system using boiloff helium gas from the dewar, and having the ability to prevent sloshing of liquid helium, would also be tested.

  19. Flight Tests of a Ministick Controller in an F/A-18 Airplane

    Science.gov (United States)

    Stoliker, Patrick C.; Carter, John

    2003-01-01

    In March of 1999, five pilots performed flight tests to evaluate the handling qualities of an F/A-18 research airplane equipped with a small-displacement center stick (ministick) controller that had been developed for the JAS 39 Gripen airplane (a fighter/attack/ reconnaissance airplane used by the Swedish air force). For these tests, the ministick was installed in the aft cockpit (see figure) and production support flight control computers (PSFCCs) were used as interfaces between the controller hardware and the standard F/A-18 flight-control laws. The primary objective of the flight tests was to assess any changes in handling qualities of the F/A-18 airplane attributable to the mechanical characteristics of the ministick. The secondary objective was to demonstrate the capability of the PSFCCs to support flight-test experiments.

  20. Ares I-X Flight Test Validation of Control Design Tools in the Frequency-Domain

    Science.gov (United States)

    Johnson, Matthew; Hannan, Mike; Brandon, Jay; Derry, Stephen

    2011-01-01

    A major motivation of the Ares I-X flight test program was to Design for Data, in order to maximize the usefulness of the data recorded in support of Ares I modeling and validation of design and analysis tools. The Design for Data effort was intended to enable good post-flight characterizations of the flight control system, the vehicle structural dynamics, and also the aerodynamic characteristics of the vehicle. To extract the necessary data from the system during flight, a set of small predetermined Programmed Test Inputs (PTIs) was injected directly into the TVC signal. These PTIs were designed to excite the necessary vehicle dynamics while exhibiting a minimal impact on loads. The method is similar to common approaches in aircraft flight test programs, but with unique launch vehicle challenges due to rapidly changing states, short duration of flight, a tight flight envelope, and an inability to repeat any test. This paper documents the validation effort of the stability analysis tools to the flight data which was performed by comparing the post-flight calculated frequency response of the vehicle to the frequency response calculated by the stability analysis tools used to design and analyze the preflight models during the control design effort. The comparison between flight day frequency response and stability tool analysis for flight of the simulated vehicle shows good agreement and provides a high level of confidence in the stability analysis tools for use in any future program. This is true for both a nominal model as well as for dispersed analysis, which shows that the flight day frequency response is enveloped by the vehicle s preflight uncertainty models.

  1. Flight test techniques for validating simulated nuclear electromagnetic pulse aircraft responses

    Science.gov (United States)

    Winebarger, R. M.; Neely, W. R., Jr.

    1984-01-01

    An attempt has been made to determine the effects of nuclear EM pulses (NEMPs) on aircraft systems, using a highly instrumented NASA F-106B to document the simulated NEMP environment at the Kirtland Air Force Base's Vertically Polarized Dipole test facility. Several test positions were selected so that aircraft orientation relative to the test facility would be the same in flight as when on the stationary dielectric stand, in order to validate the dielectric stand's use in flight configuration simulations. Attention is given to the flight test portions of the documentation program.

  2. Pre-flight physical simulation test of HIMES reentry test vehicle

    Science.gov (United States)

    Kawaguchi, Jun'ichiro; Inatani, Yoshifumi; Yonemoto, Koichi; Hosokawa, Shigeru

    ISAS is now developing a small reentry test vehicle, which is 2m long with a 1.5m wing span and weighs about 170 kg, for the purpose of exploring high angle-of-attack aerodynamic attitude control issue in supersonic and hypersonic speed. The flight test, employing 'Rockoon' launch system, is planned as a preliminary design verification for a fully reusable winged rocket named HIMES (Highly Maneuverable Experimental Space) vehicle. This paper describes the results of preflight ground test using a motion table system. This ground system test is called 'physical simulation' aimed at: (1) functional verification of side-jet system, aerodynamic surface actuators, battery and onboard avionics; and (2) guidance and control law evaluation, in total hardware-in-the-loop system. The pressure of side-jet nozzles was measured to provide exact thrust characteristics of reaction control. The dynamics of vehicle motion was calculated in real-time by the ground simulation computer.

  3. Synthetic and Enhanced Vision Systems for NextGen (SEVS) Simulation and Flight Test Performance Evaluation

    Science.gov (United States)

    Shelton, Kevin J.; Kramer, Lynda J.; Ellis,Kyle K.; Rehfeld, Sherri A.

    2012-01-01

    The Synthetic and Enhanced Vision Systems for NextGen (SEVS) simulation and flight tests are jointly sponsored by NASA's Aviation Safety Program, Vehicle Systems Safety Technology project and the Federal Aviation Administration (FAA). The flight tests were conducted by a team of Honeywell, Gulfstream Aerospace Corporation and NASA personnel with the goal of obtaining pilot-in-the-loop test data for flight validation, verification, and demonstration of selected SEVS operational and system-level performance capabilities. Nine test flights (38 flight hours) were conducted over the summer and fall of 2011. The evaluations were flown in Gulfstream.s G450 flight test aircraft outfitted with the SEVS technology under very low visibility instrument meteorological conditions. Evaluation pilots flew 108 approaches in low visibility weather conditions (600 ft to 2400 ft visibility) into various airports from Louisiana to Maine. In-situ flight performance and subjective workload and acceptability data were collected in collaboration with ground simulation studies at LaRC.s Research Flight Deck simulator.

  4. Phenotypic identification of Porphyromonas gingivalis validated with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

    NARCIS (Netherlands)

    Rams, Thomas E; Sautter, Jacqueline D; Getreu, Adam; van Winkelhoff, Arie J

    OBJECTIVE: Porphyromonas gingivalis is a major bacterial pathogen in human periodontitis. This study used matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to assess the accuracy of a rapid phenotypic identification scheme for detection of cultivable P.

  5. Identification of molecules in graphite furnace by laser ionization time-of-flight mass spectrometry: sulfur and chlorine containing compounds

    CSIR Research Space (South Africa)

    Raseleka, RM

    2004-01-01

    Full Text Available An electro thermal vaporizer (ETV) coupled to a time-of-flight mass spectrometer (TOF-MS) with laser ionization (LI) was applied to the identification of molecules from sulphur and chlorine matrices in the furnace. An interface was developed...

  6. Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of molds of the Fusarium genus.

    Science.gov (United States)

    Triest, David; Stubbe, Dirk; De Cremer, Koen; Piérard, Denis; Normand, Anne-Cécile; Piarroux, Renaud; Detandt, Monique; Hendrickx, Marijke

    2015-02-01

    The rates of infection with Fusarium molds are increasing, and a diverse number of Fusarium spp. belonging to different species complexes can cause infection. Conventional species identification in the clinical laboratory is time-consuming and prone to errors. We therefore evaluated whether matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a useful alternative. The 289 Fusarium strains from the Belgian Coordinated Collections of Microorganisms (BCCM)/Institute of Hygiene and Epidemiology Mycology (IHEM) culture collection with validated sequence-based identities and comprising 40 species were used in this study. An identification strategy was developed, applying a standardized MALDI-TOF MS assay and an in-house reference spectrum database. In vitro antifungal testing was performed to assess important differences in susceptibility between clinically relevant species/species complexes. We observed that no incorrect species complex identifications were made by MALDI-TOF MS, and 82.8% of the identifications were correct to the species level. This success rate was increased to 91% by lowering the cutoff for identification. Although the identification of the correct species complex member was not always guaranteed, antifungal susceptibility testing showed that discriminating between Fusarium species complexes can be important for treatment but is not necessarily required between members of a species complex. With this perspective, some Fusarium species complexes with closely related members can be considered as a whole, increasing the success rate of correct identifications to 97%. The application of our user-friendly MALDI-TOF MS identification approach resulted in a dramatic improvement in both time and accuracy compared to identification with the conventional method. A proof of principle of our MALDI-TOF MS approach in the clinical setting using recently isolated Fusarium strains demonstrated its validity. Copyright © 2015

  7. Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Molds of the Fusarium Genus

    Science.gov (United States)

    Stubbe, Dirk; De Cremer, Koen; Piérard, Denis; Normand, Anne-Cécile; Piarroux, Renaud; Detandt, Monique; Hendrickx, Marijke

    2014-01-01

    The rates of infection with Fusarium molds are increasing, and a diverse number of Fusarium spp. belonging to different species complexes can cause infection. Conventional species identification in the clinical laboratory is time-consuming and prone to errors. We therefore evaluated whether matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a useful alternative. The 289 Fusarium strains from the Belgian Coordinated Collections of Microorganisms (BCCM)/Institute of Hygiene and Epidemiology Mycology (IHEM) culture collection with validated sequence-based identities and comprising 40 species were used in this study. An identification strategy was developed, applying a standardized MALDI-TOF MS assay and an in-house reference spectrum database. In vitro antifungal testing was performed to assess important differences in susceptibility between clinically relevant species/species complexes. We observed that no incorrect species complex identifications were made by MALDI-TOF MS, and 82.8% of the identifications were correct to the species level. This success rate was increased to 91% by lowering the cutoff for identification. Although the identification of the correct species complex member was not always guaranteed, antifungal susceptibility testing showed that discriminating between Fusarium species complexes can be important for treatment but is not necessarily required between members of a species complex. With this perspective, some Fusarium species complexes with closely related members can be considered as a whole, increasing the success rate of correct identifications to 97%. The application of our user-friendly MALDI-TOF MS identification approach resulted in a dramatic improvement in both time and accuracy compared to identification with the conventional method. A proof of principle of our MALDI-TOF MS approach in the clinical setting using recently isolated Fusarium strains demonstrated its validity. PMID:25411180

  8. Flight Test Results from the Rake Airflow Gage Experiment on the F-15B Airplane

    Science.gov (United States)

    Frederick, Michael A.; Ratnayake, Nalin A.

    2011-01-01

    The Rake Airflow Gage Experiment involves a flow-field survey rake that was flown on the Propulsion Flight Test Fixture at the NASA Dryden Flight Research Center using the Dryden F-15B research test bed airplane. The objective of this flight test was to ascertain the flow-field angularity, local Mach number profile, total pressure distortion, and dynamic pressure at the aerodynamic interface plane of the Channeled Centerbody Inlet Experiment. This new mixed-compression, supersonic inlet is planned for flight test in the near term. Knowledge of the flow-field characteristics at this location underneath the airplane is essential to flight test planning and computational modeling of the new inlet, anairplane, flying at a free-stream Mach number of 1.65 and a pressure altitude of 40,000 ft, would achieve the desired local Mach number for the future inlet flight test. Interface plane distortion levels of 2 percent and a local angle of attack of -2 deg were observed at this condition. Alternative flight conditions for future testing and an exploration of certain anomalous data also are provided.

  9. Subscale Flight Testing for Aircraft Loss of Control: Accomplishments and Future Directions

    Science.gov (United States)

    Cox, David E.; Cunningham, Kevin; Jordan, Thomas L.

    2012-01-01

    Subscale flight-testing provides a means to validate both dynamic models and mitigation technologies in the high-risk flight conditions associated with aircraft loss of control. The Airborne Subscale Transport Aircraft Research (AirSTAR) facility was designed to be a flexible and efficient research facility to address this type of flight-testing. Over the last several years (2009-2011) it has been used to perform 58 research flights with an unmanned, remotely-piloted, dynamically-scaled airplane. This paper will present an overview of the facility and its architecture and summarize the experimental data collected. All flights to date have been conducted within visual range of a safety observer. Current plans for the facility include expanding the test volume to altitudes and distances well beyond visual range. The architecture and instrumentation changes associated with this upgrade will also be presented.

  10. An analysis of unit tests of a flight software product line

    NARCIS (Netherlands)

    Ganesan, D.; Lindvall, M.; McComas, D.; Bartholomew, M.; Slegel, S.; Medina, B.; Krikhaar, R.; Verhoef, C.; Dharmalingam, G.; Montgomery, L.P.

    2013-01-01

    This paper presents an analysis of the unit testing approach developed and used by the Core Flight Software System (CFS) product line team at the NASA Goddard Space Flight Center (GSFC). The goal of the analysis is to understand, review, and recommend strategies for improving the CFS' existing unit

  11. MSFC Doppler Lidar Science experiments and operations plans for 1981 airborne test flight

    Science.gov (United States)

    Fichtl, G. H.; Bilbro, J. W.; Kaufman, J. W.

    1981-01-01

    The flight experiment and operations plans for the Doppler Lidar System (DLS) are provided. Application of DLS to the study of severe storms and local weather penomena is addressed. Test plans involve 66 hours of flight time. Plans also include ground based severe storm and local weather data acquisition.

  12. A comparison of theory and flight test of the BO 105/BMR in hover and forward flight

    Science.gov (United States)

    Mirick, Paul H.

    1988-01-01

    Four cases were selected for comparison with theoretical predictions using stability data obtained during the flight test of the Bearingless Main Rotor (BMR) on a Messerschmidt-Boelkow-Blohm BO 105 helicopter. The four cases selected form the flight test included two ground resonance cases and two air resonance cases. The BMR used four modified BO 105 blades attached to a bearingless hub. The hub consisted of dual fiberglass C-channel beams attached to the hub center at 0.0238R and attached to the blade root at 0.25R with blade pitch control provided by a torque tube. Analyses from Bell Helicopter Textron, Boeing Vertol, and Sikorsky Aircraft were compared with the data and the correlation ranged from very poor-to-poor to poor-to-fair.

  13. Dive Angle Sensitivity Analysis for Flight Test Safety and Efficiency

    Science.gov (United States)

    2010-03-01

    22]. Raymer points out that most flutter modes are driven by improper balancing of control surfaces, but these flutter modes can be excited...description1.html [cited 2 Feb. 2010] [10] Nelson, Robert C., Flight Stability and Automatic Control, 2nd ed., McGraw-Hill Higher Education, Boston...Patuxent River Naval Air Station MD, May 1992, Chap. 10 [15] Raymer , Daniel, P., Aircraft Design: A Conceptual Approach, 4th ed., AIAA Education

  14. Operational Overview for UAS Integration in the NAS Project Flight Test Series 3

    Science.gov (United States)

    Valkov, Steffi B.; Sternberg, Daniel; Marston, Michael

    2018-01-01

    The National Aeronautics and Space Administration Unmanned Aircraft Systems Integration in the National Airspace System Project has conducted a series of flight tests intended to support the reduction of barriers that prevent unmanned aircraft from flying without the required waivers from the Federal Aviation Administration. The 2015 Flight Test Series 3, supported two separate test configurations. The first configuration investigated the timing of Detect and Avoid alerting thresholds using a radar equipped unmanned vehicle and multiple live intruders flown at varying encounter geometries.

  15. Modelling of XCO2 Surfaces Based on Flight Tests of TanSat Instruments

    Directory of Open Access Journals (Sweden)

    Li Li Zhang

    2016-11-01

    Full Text Available The TanSat carbon satellite is to be launched at the end of 2016. In order to verify the performance of its instruments, a flight test of TanSat instruments was conducted in Jilin Province in September, 2015. The flight test area covered a total area of about 11,000 km2 and the underlying surface cover included several lakes, forest land, grassland, wetland, farmland, a thermal power plant and numerous cities and villages. We modeled the column-average dry-air mole fraction of atmospheric carbon dioxide (XCO2 surface based on flight test data which measured the near- and short-wave infrared (NIR reflected solar radiation in the absorption bands at around 760 and 1610 nm. However, it is difficult to directly analyze the spatial distribution of XCO2 in the flight area using the limited flight test data and the approximate surface of XCO2, which was obtained by regression modeling, which is not very accurate either. We therefore used the high accuracy surface modeling (HASM platform to fill the gaps where there is no information on XCO2 in the flight test area, which takes the approximate surface of XCO2 as its driving field and the XCO2 observations retrieved from the flight test as its optimum control constraints. High accuracy surfaces of XCO2 were constructed with HASM based on the flight’s observations. The results showed that the mean XCO2 in the flight test area is about 400 ppm and that XCO2 over urban areas is much higher than in other places. Compared with OCO-2’s XCO2, the mean difference is 0.7 ppm and the standard deviation is 0.95 ppm. Therefore, the modelling of the XCO2 surface based on the flight test of the TanSat instruments fell within an expected and acceptable range.

  16. Preliminary test results of a flight management algorithm for fuel conservative descents in a time based metered traffic environment. [flight tests of an algorithm to minimize fuel consumption of aircraft based on flight time

    Science.gov (United States)

    Knox, C. E.; Cannon, D. G.

    1979-01-01

    A flight management algorithm designed to improve the accuracy of delivering the airplane fuel efficiently to a metering fix at a time designated by air traffic control is discussed. The algorithm provides a 3-D path with time control (4-D) for a test B 737 airplane to make an idle thrust, clean configured descent to arrive at the metering fix at a predetermined time, altitude, and airspeed. The descent path is calculated for a constant Mach/airspeed schedule from linear approximations of airplane performance with considerations given for gross weight, wind, and nonstandard pressure and temperature effects. The flight management descent algorithms and the results of the flight tests are discussed.

  17. Configuration management issues and objectives for a real-time research flight test support facility

    Science.gov (United States)

    Yergensen, Stephen; Rhea, Donald C.

    1988-01-01

    Presented are some of the critical issues and objectives pertaining to configuration management for the NASA Western Aeronautical Test Range (WATR) of Ames Research Center. The primary mission of the WATR is to provide a capability for the conduct of aeronautical research flight test through real-time processing and display, tracking, and communications systems. In providing this capability, the WATR must maintain and enforce a configuration management plan which is independent of, but complimentary to, various research flight test project configuration management systems. A primary WATR objective is the continued development of generic research flight test project support capability, wherein the reliability of WATR support provided to all project users is a constant priority. Therefore, the processing of configuration change requests for specific research flight test project requirements must be evaluated within a perspective that maintains this primary objective.

  18. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    Science.gov (United States)

    Stevenson, Lindsay G; Drake, Steven K; Murray, Patrick R

    2010-02-01

    Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid, accurate method for identifying bacteria and fungi recovered on agar culture media. We report herein a method for the direct identification of bacteria in positive blood culture broths by MALDI-TOF mass spectrometry. A total of 212 positive cultures were examined, representing 32 genera and 60 species or groups. The identification of bacterial isolates by MALDI-TOF mass spectrometry was compared with biochemical testing, and discrepancies were resolved by gene sequencing. No identification (spectral score of blood culture broth. Of the bacteria with a spectral score of > or = 1.7, 162 (95.3%) of 170 isolates were correctly identified. All 8 isolates of Streptococcus mitis were misidentified as being Streptococcus pneumoniae isolates. This method provides a rapid, accurate, definitive identification of bacteria within 1 h of detection in positive blood cultures with the caveat that the identification of S. pneumoniae would have to be confirmed by an alternative test.

  19. [Separation and identification of bovine lactoferricin by high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/ time of flight mass spectrometry].

    Science.gov (United States)

    An, Meichen; Liu, Ning

    2010-02-01

    A high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry (HPLC-MALDI-TOF/TOF MS) method was developed for the separation and identification of bovine lactoferricin (LfcinB). Bovine lactoferrin was hydrolyzed by pepsin and then separated by ion exchange chromatography and reversed-phase liquid chromatography (RP-LC). The antibacterial activities of the fractions from RP-LC separation were determined and the protein concentration of the fraction with the highest activity was measured, whose sequence was indentified by MALDI-TOF/TOF MS. The relative molecular mass of LfcinB was 3 124.89 and the protein concentration was 18.20 microg/mL. The method of producing LfcinB proposed in this study has fast speed, high accuracy and high resolution.

  20. NASA-FAA helicopter Microwave Landing System curved path flight test

    Science.gov (United States)

    Swenson, H. N.; Hamlin, J. R.; Wilson, G. W.

    1984-01-01

    An ongoing series of joint NASA/FAA helicopter Microwave Landing System (MLS) flight tests was conducted at Ames Research Center. This paper deals with tests done from the spring through the fall of 1983. This flight test investigated and developed solutions to the problem of manually flying curved-path and steep glide slope approaches into the terminal area using the MLS and flight director guidance. An MLS-equipped Bell UH-1H helicopter flown by NASA test pilots was used to develop approaches and procedures for flying these approaches. The approaches took the form of Straight-in, U-turn, and S-turn flightpaths with glide slopes of 6 deg, 9 deg, and 12 deg. These procedures were evaluated by 18 pilots from various elements of the helicopter community, flying a total of 221 hooded instrument approaches. Flying these curved path and steep glide slopes was found to be operationally acceptable with flight director guidance using the MLS.

  1. Preliminary studies of microchannel plate photomultiplier tube neutron detectors for flight test applications

    International Nuclear Information System (INIS)

    Dolan, K.W.

    1978-10-01

    Electrical, mechanical, thermal, and neutron response data indicate that microchannel plate photomultiplier tubes are viable candidates as miniature, ruggedized neutron detectors for flight test applications in future weapon systems

  2. Executive Summary of Propulsion on the Orion Abort Flight-Test Vehicles

    Science.gov (United States)

    Jones, Daniel S.; Brooks, Syri J.; Barnes, Marvin W.; McCauley, Rachel J.; Wall, Terry M.; Reed, Brian D.; Duncan, C. Miguel

    2012-01-01

    The National Aeronautics and Space Administration Orion Flight Test Office was tasked with conducting a series of flight tests in several launch abort scenarios to certify that the Orion Launch Abort System is capable of delivering astronauts aboard the Orion Crew Module to a safe environment, away from a failed booster. The first of this series was the Orion Pad Abort 1 Flight-Test Vehicle, which was successfully flown on May 6, 2010 at the White Sands Missile Range in New Mexico. This report provides a brief overview of the three propulsive subsystems used on the Pad Abort 1 Flight-Test Vehicle. An overview of the propulsive systems originally planned for future flight-test vehicles is also provided, which also includes the cold gas Reaction Control System within the Crew Module, and the Peacekeeper first stage rocket motor encased within the Abort Test Booster aeroshell. Although the Constellation program has been cancelled and the operational role of the Orion spacecraft has significantly evolved, lessons learned from Pad Abort 1 and the other flight-test vehicles could certainly contribute to the vehicle architecture of many future human-rated space launch vehicles

  3. Environmental control and life support testing at the Marshall Space Flight Center

    Science.gov (United States)

    Schunk, Richard G.; Humphries, William R.

    1987-01-01

    The Space Station Environmental Control and Life Support System (ECLSS) test program at the Marshall Space Flight Center (MSFC) is addressed. The immediate goals and current activities of the test program are discussed. Also described are the Core Module Integration Facility (CMIF) and the initial ECLSS test configuration. Future plans for the ECLSS test program and the CMIF are summarized.

  4. Qualification and Flight Test of Non-Chrome Primers for C-130 Aircraft

    Science.gov (United States)

    2011-08-17

    system  Significant hexavalent chrome reduction in finish system  Potential exposure level of spray applied chromated conversion coating not as...Lockheed Martin Aeronautics Company Qualification and Flight Test of Non- Chrome Primers for C-130 Aircraft Scott Jones Lockheed Martin...00-2011 to 00-00-2011 4. TITLE AND SUBTITLE Qualification and Flight Test of Non- Chrome Primers for C-130 Aircraft 5a. CONTRACT NUMBER 5b. GRANT

  5. Finding of No Significant Impact and Environmental Assessment for Flight Test to the Edge of Space

    Science.gov (United States)

    2008-12-01

    Runway 22 or on Rogers Dry Lakebed at Edwards AFB. 17 On the basis of the findings of the Environmental Assessment, no significant impact to human...FLIGHT TEST CENTER Environmental Assessment for Flight Test to the Edge of Space Page 5-3 Bowles, A.E., S. Eckert, L . Starke, E. Berg, L . Wolski, and...Numbers. Anne Choate, Laura 20 Pederson , Jeremy Scharfenberg, Henry Farland. Washington, D.C. September. 21 Jeppesen Sanderson, Incorporated 22

  6. In-flight and ground testing of single event upset sensitivity in static RAMs

    International Nuclear Information System (INIS)

    Johansson, K.; Dyreklev, P.; Granbom, B.; Calvet, C.; Fourtine, S.; Feuillatre, O.

    1998-01-01

    This paper presents the results from in-flight measurements of single event upsets (SEU) in static random access memories (SRAM) caused by the atmospheric radiation environment at aircraft altitudes. The memory devices were carried on commercial airlines at high altitude and mainly high latitudes. The SEUs were monitored by a Component Upset Test Equipment (CUTE), designed for this experiment. The in flight results are compared to ground based testing with neutrons from three different sources

  7. Design Challenges Encountered in a Propulsion-Controlled Aircraft Flight Test Program

    Science.gov (United States)

    Maine, Trindel; Burken, John; Burcham, Frank; Schaefer, Peter

    1994-01-01

    The NASA Dryden Flight Research Center conducted flight tests of a propulsion-controlled aircraft system on an F-15 airplane. This system was designed to explore the feasibility of providing safe emergency landing capability using only the engines to provide flight control in the event of a catastrophic loss of conventional flight controls. Control laws were designed to control the flightpath and bank angle using only commands to the throttles. Although the program was highly successful, this paper highlights some of the challenges associated with using engine thrust as a control effector. These challenges include slow engine response time, poorly modeled nonlinear engine dynamics, unmodeled inlet-airframe interactions, and difficulties with ground effect and gust rejection. Flight and simulation data illustrate these difficulties.

  8. Torque Tension Testing of Fasteners used for NASA Flight Hardware Applications

    Science.gov (United States)

    Hemminger, Edgar G.; Posey, Alan J.; Dube, Michael J.

    2014-01-01

    The effect of various lubricants and other compounds on fastener torque-tension relationships is evaluated. Testing was performed using a unique test apparatus developed by Posey at the NASA Goddard Space Flight Center. A description of the test methodology, including associated data collection and analysis will be presented. Test results for 300 series CRES and A286 heat resistant fasteners, torqued into various types of inserts will be presented. The primary objective of this testing was to obtain torque-tension data for use on NASA flight projects.

  9. Development of a test and flight engineering oriented language, phase 3

    Science.gov (United States)

    Kamsler, W. F.; Case, C. W.; Kinney, E. L.; Gyure, J.

    1970-01-01

    Based on an analysis of previously developed test oriented languages and a study of test language requirements, a high order language was designed to enable test and flight engineers to checkout and operate the proposed space shuttle and other NASA vehicles and experiments. The language is called ALOFT (a language oriented to flight engineering and testing). The language is described, its terminology is compared to similar terms in other test languages, and its features and utilization are discussed. The appendix provides the specifications for ALOFT.

  10. Performance of matrix-assisted laser desorption-time of flight mass spectrometry for identification of clinical yeast isolates

    DEFF Research Database (Denmark)

    Rosenvinge, Flemming S; Dzajic, Esad; Knudsen, Elisa

    2013-01-01

    Accurate and fast yeast identification is important when treating patients with invasive fungal disease as susceptibility to antifungal agents is highly species related. Matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF-MS) provides a powerful tool with a clear potential...... spectra output, all 13 isolates were correctly identified, resulting in an overall identification performance of 92%. No misidentifications occurred with the two systems. Of the routine isolates one laboratory identified 99/99 (100%) and 90/99 (91%) to species level by Saramis/Axima and conventional...... identification, respectively, whereas the other laboratory identified 83/98 (85%) to species level by both BioTyper/Bruker and conventional identification. Both MALDI-TOF-MS systems are fast, have built-in databases that cover the majority of clinically relevant Candida species, and have an accuracy...

  11. Testing for Nuclear Thermal Propulsion Systems: Identification of Technologies for Effluent Treatment in Test Facilities

    Data.gov (United States)

    National Aeronautics and Space Administration — Key steps to ensure identification of relevant effluent treatment technologies for Nuclear Thermal Propulsion (NTP) testing include the following. 1. Review of...

  12. Identification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system.

    Science.gov (United States)

    Richter, S S; Sercia, L; Branda, J A; Burnham, C-A D; Bythrow, M; Ferraro, M J; Garner, O B; Ginocchio, C C; Jennemann, R; Lewinski, M A; Manji, R; Mochon, A B; Rychert, J A; Westblade, L F; Procop, G W

    2013-12-01

    This multicenter study evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry identifications from the VITEK MS system (bioMérieux, Marcy l'Etoile, France) for Enterobacteriaceae typically encountered in the clinical laboratory. Enterobacteriaceae isolates (n = 965) representing 17 genera and 40 species were analyzed on the VITEK MS system (database v2.0), in accordance with the manufacturer's instructions. Colony growth (≤72 h) was applied directly to the target slide. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry before mass spectrometry analysis. On the basis of the confidence level, the VITEK MS system provided a species, genus only, or no identification for each isolate. The accuracy of the mass spectrometric identification was compared to 16S rRNA gene sequencing performed at MIDI Labs (Newark, DE). Supplemental phenotypic testing was performed at bioMérieux when necessary. The VITEK MS result agreed with the reference method identification for 96.7% of the 965 isolates tested, with 83.8% correct to the species level and 12.8% limited to a genus-level identification. There was no identification for 1.7% of the isolates. The VITEK MS system misidentified 7 isolates (0.7 %) as different genera. Three Pantoea agglomerans isolates were misidentified as Enterobacter spp. and single isolates of Enterobacter cancerogenus, Escherichia hermannii, Hafnia alvei, and Raoultella ornithinolytica were misidentified as Klebsiella oxytoca, Citrobacter koseri, Obesumbacterium proteus, and Enterobacter aerogenes, respectively. Eight isolates (0.8 %) were misidentified as a different species in the correct genus. The VITEK MS system provides reliable mass spectrometric identifications for Enterobacteriaceae.

  13. Yeast identification: reassessment of assimilation tests as sole universal identifiers.

    Science.gov (United States)

    Spencer, J; Rawling, S; Stratford, M; Steels, H; Novodvorska, M; Archer, D B; Chandra, S

    2011-11-01

    To assess whether assimilation tests in isolation remain a valid method of identification of yeasts, when applied to a wide range of environmental and spoilage isolates. Seventy-one yeast strains were isolated from a soft drinks factory. These were identified using assimilation tests and by D1/D2 rDNA sequencing. When compared to sequencing, assimilation test identifications (MicroLog™) were 18·3% correct, a further 14·1% correct within the genus and 67·6% were incorrectly identified. The majority of the latter could be attributed to the rise in newly reported yeast species. Assimilation tests alone are unreliable as a universal means of yeast identification, because of numerous new species, variability of strains and increasing coincidence of assimilation profiles. Assimilation tests still have a useful role in the identification of common species, such as the majority of clinical isolates. It is probable, based on these results, that many yeast identifications reported in older literature are incorrect. This emphasizes the crucial need for accurate identification in present and future publications. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

  14. A simple method for rapid microbial identification from positive monomicrobial blood culture bottles through matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Lin, Jung-Fu; Ge, Mao-Cheng; Liu, Tsui-Ping; Chang, Shih-Cheng; Lu, Jang-Jih

    2017-06-30

    Rapid identification of microbes in the bloodstream is crucial in managing septicemia because of its high disease severity, and direct identification from positive blood culture bottles through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can shorten the turnaround time. Therefore, we developed a simple method for rapid microbiological identification from positive blood cultures by using MALDI-TOF MS. We modified previously developed methods to propose a faster, simpler and more economical method, which includes centrifugation and hemolysis. Specifically, our method comprises two-stage centrifugation with gravitational acceleration (g) at 600g and 3000g, followed by the addition of a lysis buffer and another 3000g centrifugation. In total, 324 monomicrobial bacterial cultures were identified. The success rate of species identification was 81.8%, which is comparable with other complex methods. The identification success rate was the highest for Gram-negative aerobes (85%), followed by Gram-positive aerobes (78.2%) and anaerobes (67%). The proposed method requires less than 10 min, costs less than US$0.2 per usage, and facilitates batch processing. We conclude that this method is feasible for clinical use in microbiology laboratories, and can serve as a reference for treatments or further complementary diagnostic testing. Copyright © 2017. Published by Elsevier B.V.

  15. Shuttle Rudder/Speed Brake Power Drive Unit (PDU) Gear Scuffing Tests With Flight Gears

    Science.gov (United States)

    Proctor, Margaret P.; Oswald, Fred B.; Krants, Timothy L.

    2005-01-01

    Scuffing-like damage has been found on the tooth surfaces of gears 5 and 6 of the NASA space shuttle rudder/speed brake power drive unit (PDU) number 2 after the occurrence of a transient back-driving event in flight. Tests were conducted using a pair of unused spare flight gears in a bench test at operating conditions up to 2866 rpm and 1144 in.-lb at the input ring gear and 14,000 rpm and 234 in.-lb at the output pinion gear, corresponding to a power level of 52 hp. This test condition exceeds the maximum estimated conditions expected in a backdriving event thought to produce the scuffing damage. Some wear marks were produced, but they were much less severe than the scuffing damaged produced during shuttle flight. Failure to produce scuff damage like that found on the shuttle may be due to geometrical variations between the scuffed gears and the gears tested herein, more severe operating conditions during the flight that produced the scuff than estimated, the order of the test procedures, the use of new hydraulic oil, differences between the dynamic response of the flight gearbox and the bench-test gearbox, or a combination of these. This report documents the test gears, apparatus, and procedures, summarizes the test results, and includes a discussion of the findings, conclusions, and recommendations.

  16. Identification of wheat varieties using matrix-assisted laserdesorption/ionisation time-of-flight mass spectrometry and anartificial neural network

    DEFF Research Database (Denmark)

    Bloch, Helle Aagaard; Kesmir, Can; Petersen, Marianne Kjerstine

    1999-01-01

    of this novelmethod with respect to various experimental parameters has been tested. The results can be summarised: (i)With this approach 97% of the wheat varieties can be classified correctly with a corresponding correlationcoefficient of 1.0, (ii) The method is fast since the time of extracting gliadins from flour......A novel tool for variety identification of wheat (Triticum aestivum L.) has been developed: an artificialneural network (ANN) is used to classify the gliadin fraction analysed by matrix-assisted laserdesorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS). The robustness...... by the identity of the operator making theanalysis. This study demonstrates that a combination of an ANN and MALDI-TOFMS analysis of thegliadin fraction provides a fast and reliable tool for the variety identification of wheat. Copyright 1999 JohnWiley & Sons, Ltd....

  17. Non Nuclear Testing of Reactor Systems In The Early Flight Fission Test Facilities (EFF-TF)

    International Nuclear Information System (INIS)

    Van Dyke, Melissa; Martin, James

    2004-01-01

    The Early Flight Fission-Test Facility (EFF-TF) can assist in the design and development of systems through highly effective non-nuclear testing of nuclear systems when technical issues associated with near-term space fission systems are 'non-nuclear' in nature (e.g. system's nuclear operations are understood). For many systems, thermal simulators can be used to closely mimic fission heat deposition. Axial power profile, radial power profile, and fuel pin thermal conductivity can be matched. In addition to component and subsystem testing, operational and lifetime issues associated with the steady state and transient performance of the integrated reactor module can be investigated. Instrumentation at the EFF-TF allows accurate measurement of temperature, pressure, strain, and bulk core deformation (useful for accurately simulating nuclear behavior). Ongoing research at the EFF-TF is geared towards facilitating research, development, system integration, and system utilization via cooperative efforts with DOE laboratories, industry, universities, and other Nasa centers. This paper describes the current efforts for the latter portion of 2003 and beginning of 2004. (authors)

  18. Development of a new protocol for rapid bacterial identification and susceptibility testing directly from urine samples.

    Science.gov (United States)

    Zboromyrska, Y; Rubio, E; Alejo, I; Vergara, A; Mons, A; Campo, I; Bosch, J; Marco, F; Vila, J

    2016-06-01

    The current gold standard method for the diagnosis of urinary tract infections (UTI) is urine culture that requires 18-48 h for the identification of the causative microorganisms and an additional 24 h until the results of antimicrobial susceptibility testing (AST) are available. The aim of this study was to shorten the time of urine sample processing by a combination of flow cytometry for screening and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for bacterial identification followed by AST directly from urine. The study was divided into two parts. During the first part, 675 urine samples were processed by a flow cytometry device and a cut-off value of bacterial count was determined to select samples for direct identification by MALDI-TOF-MS at ≥5 × 10(6) bacteria/mL. During the second part, 163 of 1029 processed samples reached the cut-off value. The sample preparation protocol for direct identification included two centrifugation and two washing steps. Direct AST was performed by the disc diffusion method if a reliable direct identification was obtained. Direct MALDI-TOF-MS identification was performed in 140 urine samples; 125 of the samples were positive by urine culture, 12 were contaminated and 3 were negative. Reliable direct identification was obtained in 108 (86.4%) of the 125 positive samples. AST was performed in 102 identified samples, and the results were fully concordant with the routine method among 83 monomicrobial infections. In conclusion, the turnaround time of the protocol described to diagnose UTI was about 1 h for microbial identification and 18-24 h for AST. Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  19. [Micron]ADS-B Detect and Avoid Flight Tests on Phantom 4 Unmanned Aircraft System

    Science.gov (United States)

    Arteaga, Ricardo; Dandachy, Mike; Truong, Hong; Aruljothi, Arun; Vedantam, Mihir; Epperson, Kraettli; McCartney, Reed

    2018-01-01

    Researchers at the National Aeronautics and Space Administration Armstrong Flight Research Center in Edwards, California and Vigilant Aerospace Systems collaborated for the flight-test demonstration of an Automatic Dependent Surveillance-Broadcast based collision avoidance technology on a small unmanned aircraft system equipped with the uAvionix Automatic Dependent Surveillance-Broadcast transponder. The purpose of the testing was to demonstrate that National Aeronautics and Space Administration / Vigilant software and algorithms, commercialized as the FlightHorizon UAS"TM", are compatible with uAvionix hardware systems and the DJI Phantom 4 small unmanned aircraft system. The testing and demonstrations were necessary for both parties to further develop and certify the technology in three key areas: flights beyond visual line of sight, collision avoidance, and autonomous operations. The National Aeronautics and Space Administration and Vigilant Aerospace Systems have developed and successfully flight-tested an Automatic Dependent Surveillance-Broadcast Detect and Avoid system on the Phantom 4 small unmanned aircraft system. The Automatic Dependent Surveillance-Broadcast Detect and Avoid system architecture is especially suited for small unmanned aircraft systems because it integrates: 1) miniaturized Automatic Dependent Surveillance-Broadcast hardware; 2) radio data-link communications; 3) software algorithms for real-time Automatic Dependent Surveillance-Broadcast data integration, conflict detection, and alerting; and 4) a synthetic vision display using a fully-integrated National Aeronautics and Space Administration geobrowser for three dimensional graphical representations for ownship and air traffic situational awareness. The flight-test objectives were to evaluate the performance of Automatic Dependent Surveillance-Broadcast Detect and Avoid collision avoidance technology as installed on two small unmanned aircraft systems. In December 2016, four flight tests

  20. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories.

    Science.gov (United States)

    van Veen, S Q; Claas, E C J; Kuijper, Ed J

    2010-03-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid diagnostics at low costs and can be considered an alternative for conventional biochemical and molecular identification systems in a conventional microbiological laboratory. First, we evaluated MALDI-TOF MS using 327 clinical isolates previously cultured from patient materials and identified by conventional techniques (Vitek-II, API, and biochemical tests). Discrepancies were analyzed by molecular analysis of the 16S genes. Of 327 isolates, 95.1% were identified correctly to genus level, and 85.6% were identified to species level by MALDI-TOF MS. Second, we performed a prospective validation study, including 980 clinical isolates of bacteria and yeasts. Overall performance of MALDI-TOF MS was significantly better than conventional biochemical systems for correct species identification (92.2% and 83.1%, respectively) and produced fewer incorrect genus identifications (0.1% and 1.6%, respectively). Correct species identification by MALDI-TOF MS was observed in 97.7% of Enterobacteriaceae, 92% of nonfermentative Gram-negative bacteria, 94.3% of staphylococci, 84.8% of streptococci, 84% of a miscellaneous group (mainly Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella [HACEK]), and 85.2% of yeasts. MALDI-TOF MS had significantly better performance than conventional methods for species identification of staphylococci and genus identification of bacteria belonging to HACEK group. Misidentifications by MALDI-TOF MS were clearly associated with an absence of sufficient spectra from suitable reference strains in the MALDI-TOF MS database. We conclude that MALDI-TOF MS can be implemented easily for routine identification of bacteria (except for pneumococci and viridans streptococci) and yeasts in a medical microbiological laboratory.

  1. Optical Automatic Car Identification (OACI) Field Test Program

    Science.gov (United States)

    1976-05-01

    The results of the Optical Automatic Car Identification (OACI) tests at Chicago conducted from August 16 to September 4, 1975 are presented. The main purpose of this test was to determine the suitability of optics as a principle of operation for an a...

  2. Direct structural parameter identification by modal test results

    Science.gov (United States)

    Chen, J.-C.; Kuo, C.-P.; Garba, J. A.

    1983-01-01

    A direct identification procedure is proposed to obtain the mass and stiffness matrices based on the test measured eigenvalues and eigenvectors. The method is based on the theory of matrix perturbation in which the correct mass and stiffness matrices are expanded in terms of analytical values plus a modification matrix. The simplicity of the procedure enables real time operation during the structural testing.

  3. NASA Langley Distributed Propulsion VTOL Tilt-Wing Aircraft Testing, Modeling, Simulation, Control, and Flight Test Development

    Science.gov (United States)

    Rothhaar, Paul M.; Murphy, Patrick C.; Bacon, Barton J.; Gregory, Irene M.; Grauer, Jared A.; Busan, Ronald C.; Croom, Mark A.

    2014-01-01

    Control of complex Vertical Take-Off and Landing (VTOL) aircraft traversing from hovering to wing born flight mode and back poses notoriously difficult modeling, simulation, control, and flight-testing challenges. This paper provides an overview of the techniques and advances required to develop the GL-10 tilt-wing, tilt-tail, long endurance, VTOL aircraft control system. The GL-10 prototype's unusual and complex configuration requires application of state-of-the-art techniques and some significant advances in wind tunnel infrastructure automation, efficient Design Of Experiments (DOE) tunnel test techniques, modeling, multi-body equations of motion, multi-body actuator models, simulation, control algorithm design, and flight test avionics, testing, and analysis. The following compendium surveys key disciplines required to develop an effective control system for this challenging vehicle in this on-going effort.

  4. Heart Rate Measures of Flight Test and Evaluation

    National Research Council Canada - National Science Library

    Bonner, Malcolm A; Wilson, Glenn F

    2001-01-01

    .... Because flying is a complex task, several measures are required to derive the best evaluation. This article describes the use of heart rate to augment the typical performance and subjective measures used in test and evaluation...

  5. Mode S data link transponder flight test results

    Science.gov (United States)

    1997-02-01

    The Federal Aviation Administration (FAA) William J. Hughes Technical Center is : in the unique position of having the facilities designed to test Mode S radars : and transponders. A vendor supplied an early production model of a Mode S : transponder...

  6. Control and Non-Payload Communications (CNPC) Prototype Radio Validation Flight Test Report

    Science.gov (United States)

    Shalkhauser, Kurt A.; Ishac, Joseph A.; Iannicca, Dennis C.; Bretmersky, Steven C.; Smith, Albert E.

    2017-01-01

    This report provides an overview and results from the unmanned aircraft (UA) Control and Non-Payload Communications (CNPC) Generation 5 prototype radio validation flight test campaign. The radios used in the test campaign were developed under cooperative agreement NNC11AA01A between the NASA Glenn Research Center and Rockwell Collins, Inc., of Cedar Rapids, Iowa. Measurement results are presented for flight tests over hilly terrain, open water, and urban landscape, utilizing radio sets installed into a NASA aircraft and ground stations. Signal strength and frame loss measurement data are analyzed relative to time and aircraft position, specifically addressing the impact of line-of-sight terrain obstructions on CNPC data flow. Both the radio and flight test system are described.

  7. Development of a Quadrotor Test Bed — Modelling, Parameter Identification, Controller Design and Trajectory Generation

    Directory of Open Access Journals (Sweden)

    Wei Dong

    2015-02-01

    Full Text Available In this paper, a quadrotor test bed is developed. The technical approach for this test bed is firstly proposed by utilizing a commercial quadrotor, a Vicon motion capture system and a ground station. Then, the mathematical model of the quadrotor is formulated considering aerodynamic effects, and the parameter identification approaches for this model are provided accordingly. Based on the developed model and identified parameters, a simulation environment that is consistent with the real system is developed. Subsequently, a flight control strategy and a trajectory generation method, both of which are conceptually and computationally lightweight, are developed and tested in the simulation environment. The developed algorithms are then directly transplanted to the real system, and the experimental results show that their responses in the real-time flights match well with those from the simulations. This indicates that the control algorithms developed for the quadrotor can be preliminarily verified and refined though simulations, and then directly implemented to the real system, which could significantly reduce the experimental risks and costs. Meanwhile, real-time experiments show that the developed flight controller can efficiently stabilize the quadrotor when external disturbances exist, and the trajectory generation approach can provide safe guidance for the quadrotor to fly smoothly through cluttered environments with obstacle rings. All of these features are valuable for real applications, thus demonstrating the feasibility of further development.

  8. Wind-tunnel development of an SR-71 aerospike rocket flight test configuration

    Science.gov (United States)

    Smith, Stephen C.; Shirakata, Norm; Moes, Timothy R.; Cobleigh, Brent R.; Conners, Timothy H.

    1996-01-01

    A flight experiment has been proposed to investigate the performance of an aerospike rocket motor installed in a lifting body configuration. An SR-71 airplane would be used to carry the aerospike configuration to the desired flight test conditions. Wind-tunnel tests were completed on a 4-percent scale SR-71 airplane with the aerospike pod mounted in various locations on the upper fuselage. Testing was accomplished using sting and blade mounts from Mach 0.6 to Mach 3.2. Initial test objectives included assessing transonic drag and supersonic lateral-directional stability and control. During these tests, flight simulations were run with wind-tunnel data to assess the acceptability of the configurations. Early testing demonstrated that the initial configuration with the aerospike pod near the SR-71 center of gravity was unsuitable because of large nosedown pitching moments at transonic speeds. The excessive trim drag resulting from accommodating this pitching moment far exceeded the excess thrust capability of the airplane. Wind-tunnel testing continued in an attempt to find a configuration suitable for flight test. Multiple configurations were tested. Results indicate that an aft-mounted model configuration possessed acceptable performance, stability, and control characteristics.

  9. Development of a EUV Test Facility at the Marshall Space Flight Center

    Science.gov (United States)

    West, Edward; Pavelitz, Steve; Kobayashi, Ken; Robinson, Brian; Cirtain, Johnathan; Gaskin, Jessica; Winebarger, Amy

    2011-01-01

    This paper will describe a new EUV test facility that is being developed at the Marshall Space Flight Center (MSFC) to test EUV telescopes. Two flight programs, HiC - high resolution coronal imager (sounding rocket) and SUVI - Solar Ultraviolet Imager (GOES-R), set the requirements for this new facility. This paper will discuss those requirements, the EUV source characteristics, the wavelength resolution that is expected and the vacuum chambers (Stray Light Facility, Xray Calibration Facility and the EUV test chamber) where this facility will be used.

  10. Acquisition/expulsion system for earth orbital propulsion system study. Volume 4: Flight test article

    Science.gov (United States)

    1973-01-01

    Two orbital test plans were prepared to verify one of the passive cryogenic storage tank/feedline candidate designs. One plan considered the orbital test article to be launched as a dedicated payload using an Atlas F burner launching configuration. The second plan proposed to launch the orbital test article as a secondary payload on the Titan E/Centaur proof flight. The secondary payload concept was pursued until January 1973, when work to build the hardware for this phase of the contract was terminated for lack of a sponsor for the flight. The dedicated payload launched on an Atlas F is described.

  11. Management Process of a Frequency Response Flight Test for Rotorcraft Flying Qualities Evaluation

    Directory of Open Access Journals (Sweden)

    João Otávio Falcão Arantes Filho

    2016-07-01

    Full Text Available This paper applies the frequency response methodology to characterize and analyze the flying qualities of longitudinal and lateral axes of a rotary-wing aircraft, AS355-F2. Using the results, it is possible to check the suitability of the aircraft in accordance with ADS-33E-PRF standard, whose flying qualities specifications criteria are based on parameters in the frequency domain. The key steps addressed in the study involve getting, by means of flight test data, the closed-loop dynamic responses including the design of the instrumentation and specification of the sensors to be used in the flight test campaign, the definition of the appropriate maneuvers characteristics for excitation of the aircraft, the planning and execution of the flight test to collect the data, and the proper data treatment, processing and analysis after the flight. After treatment of the collected data, single input-single output spectral analysis is performed. The results permit the analysis of the flying qualities characteristics, anticipation of the demands to which the pilot will be subjected during closed-loop evaluations and check of compliance with the aforementioned standard, within the range of consistent excitation frequencies for flight tests, setting the agility level of the test aircraft.

  12. Initial virtual flight test for a dynamically similar aircraft model with control augmentation system

    Directory of Open Access Journals (Sweden)

    Linliang Guo

    2017-04-01

    Full Text Available To satisfy the validation requirements of flight control law for advanced aircraft, a wind tunnel based virtual flight testing has been implemented in a low speed wind tunnel. A 3-degree-of-freedom gimbal, ventrally installed in the model, was used in conjunction with an actively controlled dynamically similar model of aircraft, which was equipped with the inertial measurement unit, attitude and heading reference system, embedded computer and servo-actuators. The model, which could be rotated around its center of gravity freely by the aerodynamic moments, together with the flow field, operator and real time control system made up the closed-loop testing circuit. The model is statically unstable in longitudinal direction, and it can fly stably in wind tunnel with the function of control augmentation of the flight control laws. The experimental results indicate that the model responds well to the operator’s instructions. The response of the model in the tests shows reasonable agreement with the simulation results. The difference of response of angle of attack is less than 0.5°. The effect of stability augmentation and attitude control law was validated in the test, meanwhile the feasibility of virtual flight test technique treated as preliminary evaluation tool for advanced flight vehicle configuration research was also verified.

  13. Determination of the availability of appropriate aged flight rocket motors. [captive tests to determine case bond separation and grain bore cracking

    Science.gov (United States)

    Martin, P. J.

    1974-01-01

    A program to identify surplus solid rocket propellant engines which would be available for a program of functional integrity testing was conducted. The engines are classified as: (1) upper stage and apogee engines, (2) sounding rocket and launch vehicle engines, and (3) jato, sled, and tactical engines. Nearly all the engines were available because their age exceeds the warranted shelf life. The preference for testing included tests at nominal flight conditions, at design limits, and to establish margin limits. The principal failure modes of interest were case bond separation and grain bore cracking. Data concerning the identification and characteristics of each engine are tabulated. Methods for conducting the tests are described.

  14. Two rapid pigmentation tests for identification of Cryptococcus neoformans.

    Science.gov (United States)

    Kaufmann, C S; Merz, W G

    1982-01-01

    Two tests were developed for the rapid identification of Cryptococcus neoformans based on pigment produced by the organism's phenoloxidase activity. Caffeic acid was incorporated into cornmeal agar, a medium used routinely for yeast identification. When tested on this medium, only C. neoformans isolates produced brown pigment. All other yeasts maintained their normal morphology and did not produce the reaction product. A non-medium-based test was developed for same-day identification of C. neoformans isolates. Paper strips saturated with a buffered L-beta-3,4-dihydroxyphenylalanine-ferric citrate solution were inoculated with isolates and incubated at 37 degrees C. Pigment production occurred only with C. neoformans isolates, many within 60 to 90 min. All other yeasts remained negative. PMID:7040452

  15. Identification of reduced-order model for an aeroelastic system from flutter test data

    Directory of Open Access Journals (Sweden)

    Wei Tang

    2017-02-01

    Full Text Available Recently, flutter active control using linear parameter varying (LPV framework has attracted a lot of attention. LPV control synthesis usually generates controllers that are at least of the same order as the aeroelastic models. Therefore, the reduced-order model is required by synthesis for avoidance of large computation cost and high-order controller. This paper proposes a new procedure for generation of accurate reduced-order linear time-invariant (LTI models by using system identification from flutter testing data. The proposed approach is in two steps. The well-known poly-reference least squares complex frequency (p-LSCF algorithm is firstly employed for modal parameter identification from frequency response measurement. After parameter identification, the dominant physical modes are determined by clear stabilization diagrams and clustering technique. In the second step, with prior knowledge of physical poles, the improved frequency-domain maximum likelihood (ML estimator is presented for building accurate reduced-order model. Before ML estimation, an improved subspace identification considering the poles constraint is also proposed for initializing the iterative procedure. Finally, the performance of the proposed procedure is validated by real flight flutter test data.

  16. Evaluation of Fast-Time Wake Vortex Models using Wake Encounter Flight Test Data

    Science.gov (United States)

    Ahmad, Nashat N.; VanValkenburg, Randal L.; Bowles, Roland L.; Limon Duparcmeur, Fanny M.; Gloudesman, Thijs; van Lochem, Sander; Ras, Eelco

    2014-01-01

    This paper describes a methodology for the integration and evaluation of fast-time wake models with flight data. The National Aeronautics and Space Administration conducted detailed flight tests in 1995 and 1997 under the Aircraft Vortex Spacing System Program to characterize wake vortex decay and wake encounter dynamics. In this study, data collected during Flight 705 were used to evaluate NASA's fast-time wake transport and decay models. Deterministic and Monte-Carlo simulations were conducted to define wake hazard bounds behind the wake generator. The methodology described in this paper can be used for further validation of fast-time wake models using en-route flight data, and for determining wake turbulence constraints in the design of air traffic management concepts.

  17. Linear Aerospike SR-71 Experiment (LASRE) dumps water after first in-flight cold flow test

    Science.gov (United States)

    1998-01-01

    The NASA SR-71A successfully completed its first cold flow flight as part of the NASA/Rocketdyne/Lockheed Martin Linear Aerospike SR-71 Experiment (LASRE) at NASA's Dryden Flight Research Center, Edwards, California on March 4, 1998. During a cold flow flight, gaseous helium and liquid nitrogen are cycled through the linear aerospike engine to check the engine's plumbing system for leaks and to check the engine operating characterisitics. Cold-flow tests must be accomplished successfully before firing the rocket engine experiment in flight. The SR-71 took off at 10:16 a.m. PST. The aircraft flew for one hour and fifty-seven minutes, reaching a maximum speed of Mach 1.58 before landing at Edwards at 12:13 p.m. PST. 'I think all in all we had a good mission today,' Dryden LASRE Project Manager Dave Lux said. Flight crew member Bob Meyer agreed, saying the crew 'thought it was a really good flight.' Dryden Research Pilot Ed Schneider piloted the SR-71 during the mission. Lockheed Martin LASRE Project Manager Carl Meade added, 'We are extremely pleased with today's results. This will help pave the way for the first in-flight engine data-collection flight of the LASRE.' The LASRE experiment was designed to provide in-flight data to help Lockheed Martin evaluate the aerodynamic characteristics and the handling of the SR-71 linear aerospike experiment configuration. The goal of the project was to provide in-flight data to help Lockheed Martin validate the computational predictive tools it was using to determine the aerodynamic performance of a future reusable launch vehicle. The joint NASA, Rocketdyne (now part of Boeing), and Lockheed Martin Linear Aerospike SR-71 Experiment (LASRE) completed seven initial research flights at Dryden Flight Research Center. Two initial flights were used to determine the aerodynamic characteristics of the LASRE apparatus (pod) on the back of the SR-71. Five later flights focused on the experiment itself. Two were used to cycle gaseous

  18. Rotary Balance Wind Tunnel Testing for the FASER Flight Research Aircraft

    Science.gov (United States)

    Denham, Casey; Owens, D. Bruce

    2016-01-01

    Flight dynamics research was conducted to collect and analyze rotary balance wind tunnel test data in order to improve the aerodynamic simulation and modeling of a low-cost small unmanned aircraft called FASER (Free-flying Aircraft for Sub-scale Experimental Research). The impetus for using FASER was to provide risk and cost reduction for flight testing of more expensive aircraft and assist in the improvement of wind tunnel and flight test techniques, and control laws. The FASER research aircraft has the benefit of allowing wind tunnel and flight tests to be conducted on the same model, improving correlation between wind tunnel, flight, and simulation data. Prior wind tunnel tests include a static force and moment test, including power effects, and a roll and yaw damping forced oscillation test. Rotary balance testing allows for the calculation of aircraft rotary derivatives and the prediction of steady-state spins. The rotary balance wind tunnel test was conducted in the NASA Langley Research Center (LaRC) 20-Foot Vertical Spin Tunnel (VST). Rotary balance testing includes runs for a set of given angular rotation rates at a range of angles of attack and sideslip angles in order to fully characterize the aircraft rotary dynamics. Tests were performed at angles of attack from 0 to 50 degrees, sideslip angles of -5 to 10 degrees, and non-dimensional spin rates from -0.5 to 0.5. The effects of pro-spin elevator and rudder deflection and pro- and anti-spin elevator, rudder, and aileron deflection were examined. The data are presented to illustrate the functional dependence of the forces and moments on angle of attack, sideslip angle, and angular rate for the rotary contributions to the forces and moments. Further investigation is necessary to fully characterize the control effectors. The data were also used with a steady state spin prediction tool that did not predict an equilibrium spin mode.

  19. Comparison of System Identification Methods using Ambient Bridge Test Data

    DEFF Research Database (Denmark)

    Andersen, P.; Brincker, Rune; Peeters, B.

    1999-01-01

    In this paper the performance of four different system identification methods is compared using operational data obtained from an ambient vibration test of the Swiss Z24 highway bridge. The four methods are the frequency domain based peak-picking methods, the polyreference LSCE method, the stocha......In this paper the performance of four different system identification methods is compared using operational data obtained from an ambient vibration test of the Swiss Z24 highway bridge. The four methods are the frequency domain based peak-picking methods, the polyreference LSCE method...

  20. Direct identification of bacteria from positive BacT/ALERT blood culture bottles using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.

    Science.gov (United States)

    Mestas, Javier; Felsenstein, Susanna; Bard, Jennifer Dien

    2014-11-01

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is a fast and robust method for the identification of bacteria. In this study, we evaluate the performance of a laboratory-developed lysis method (LDT) for the rapid identification of bacteria from positive BacT/ALERT blood culture bottles. Of the 168 positive bottles tested, 159 were monomicrobial, the majority of which were Gram-positive organisms (61.0% versus 39.0%). Using a cut-off score of ≥1.7, 80.4% of the organisms were correctly identified to the species level, and the identification rate of Gram-negative organisms (90.3%) was found to be significantly greater than that of Gram-positive organisms (78.4%). The simplicity and cost-effectiveness of the LDT enable it to be fully integrated into the routine workflow of the clinical microbiology laboratory, allowing for rapid identification of Gram-positive and Gram-negative bacteria within an hour of blood culture positivity. Copyright © 2014 Elsevier Inc. All rights reserved.

  1. Current Hypersonic and Space Vehicle Flight Test and Instrumentation

    Science.gov (United States)

    2015-06-22

    ground station hardware and software. B. Space- based Platforms There are already in place several satellite based options to collecting and... Transceive data over very long range at low to very high altitudes DARPA: XS-1 Ground Based Aircraft Based Space Based Future Data...412TW-PA-15264 AIR FORCE TEST CENTER EDWARDS AIR FORCE BASE , CALIFORNIA AIR FORCE MATERIEL COMMAND UNITED STATES AIR FORCE REPORT

  2. Fixed Wing Performance. Theory and Flight Test Techniques

    Science.gov (United States)

    1977-07-01

    descent data by digital computer routines is common practice at both military and contractor test facilities. The Wautomatic data reduction process...inability to reach ruider pedals or foot controls , inability to fit through emergency egrean openlngs, etc. CONTROl. DESIGN Contiols must meot various...most important controls should be the easiest to reach and manipulate. Controls should never be located such that the hand or arm manipulating the

  3. NPS Solar Cell Array Tester Cubesat Flight Testing and Integration

    Science.gov (United States)

    2014-09-01

    with current (I). P V I      (2.1) This is significant because the battery discharge test will not lineup perfectly with Figure 12...accordance with the charging procedures [13]. 3. NPS-SCAT Power Budget A power budget analysis was performed to determine if the NPS-SCAT is self...using procedures developed by Marissa Brummitt, and with the assistance of Adam Hill, NPS-SCAT Program Manager. 1. ELaNa IV Random Vibration Levels

  4. Real-time flight test data distribution and display

    Science.gov (United States)

    Nesel, Michael C.; Hammons, Kevin R.

    1988-01-01

    Enhancements to the real-time processing and display systems of the NASA Western Aeronautical Test Range are described. Display processing has been moved out of the telemetry and radar acquisition processing systems super-minicomputers into user/client interactive graphic workstations. Real-time data is provided to the workstations by way of Ethernet. Future enhancement plans include use of fiber optic cable to replace the Ethernet.

  5. Flight Test of the F/A-18 Active Aeroelastic Wing Airplane

    Science.gov (United States)

    Voracek, David

    2007-01-01

    A viewgraph presentation of flight tests performed on the F/A active aeroelastic wing airplane is shown. The topics include: 1) F/A-18 AAW Airplane; 2) F/A-18 AAW Control Surfaces; 3) Flight Test Background; 4) Roll Control Effectiveness Regions; 5) AAW Design Test Points; 6) AAW Phase I Test Maneuvers; 7) OBES Pitch Doublets; 8) OBES Roll Doublets; 9) AAW Aileron Flexibility; 10) Phase I - Lessons Learned; 11) Control Law Development and Verification & Validation Testing; 12) AAW Phase II RFCS Envelopes; 13) AAW 1-g Phase II Flight Test; 14) Region I - Subsonic 1-g Rolls; 15) Region I - Subsonic 1-g 360 Roll; 16) Region II - Supersonic 1-g Rolls; 17) Region II - Supersonic 1-g 360 Roll; 18) Region III - Subsonic 1-g Rolls; 19) Roll Axis HOS/LOS Comparison Region II - Supersonic (open-loop); 20) Roll Axis HOS/LOS Comparison Region II - Supersonic (closed-loop); 21) AAW Phase II Elevated-g Flight Test; 22) Region I - Subsonic 4-g RPO; and 23) Phase II - Lessons Learned

  6. Model and Sensor Based Nonlinear Adaptive Flight Control with Online System Identification

    NARCIS (Netherlands)

    Sun, L.G.

    2014-01-01

    Consensus exists that many loss-of-control (LOC) in flight accidents caused by severe aircraft damage or system failure could be prevented if flight performance could be recovered using the valid and remaining control authorities. However, the safe maneuverability of a post-failure aircraft will

  7. Testing command and control of the satellites in formation flight

    Science.gov (United States)

    Gheorghe, Popan; Gheorghe, Gh. Ion; Gabriel, Todoran

    2013-10-01

    The topics covered in the paper are mechatronic systems for determining the distance between the satellites and the design of the displacement system on air cushion table for satellites testing. INCDMTM has the capability to approach the collaboration within European Programms (ESA) of human exploration of outer space through mechatronic systems and accessories for telescopes, mechatronics systems used by the launchers, sensors and mechatronic systems for the robotic exploration programs of atmosphere and Mars. This research has a strong development component of industrial competitiveness many of the results of space research have direct applicability in industrial fabrication.

  8. Preliminary analysis of accelerated space flight ionizing radiation testing

    Science.gov (United States)

    Wilson, J. W.; Stock, L. V.; Carter, D. J.; Chang, C. K.

    1982-01-01

    A preliminary analysis shows that radiation dose equivalent to 30 years in the geosynchronous environment can be accumulated in a typical composite material exposed to space for 2 years or less onboard a spacecraft orbiting from perigee of 300 km out to the peak of the inner electron belt (approximately 2750 km). Future work to determine spacecraft orbits better tailored to materials accelerated testing is indicated. It is predicted that a range of 10 to the 9th power to 10 to the 10th power rads would be accumulated in 3-6 mil thick epoxy/graphite exposed by a test spacecraft orbiting in the inner electron belt. This dose is equivalent to the accumulated dose that this material would be expected to have after 30 years in a geosynchronous orbit. It is anticipated that material specimens would be brought back to Earth after 2 years in the radiation environment so that space radiation effects on materials could be analyzed by laboratory methods.

  9. Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Ferreira, L; Sánchez-Juanes, F; Porras-Guerra, I; García-García, M I; García-Sánchez, J E; González-Buitrago, J M; Muñoz-Bellido, J L

    2011-04-01

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows a fast and reliable bacterial identification from culture plates. Direct analysis of clinical samples may increase its usefulness in samples in which a fast identification of microorganisms can guide empirical treatment, such as blood cultures (BC). Three hundred and thirty BC, reported as positive by the automated BC incubation device, were processed by conventional methods for BC processing, and by a fast method based on direct MALDI-TOF MS. Three hundred and eighteen of them yield growth on culture plates, and 12 were false positive. The MALDI-TOF MS-based method reported that no peaks were found, or the absence of a reliable identification profile, in all these false positive BC. No mixed cultures were found. Among these 318 BC, we isolated 61 Gram-negatives (GN), 239 Gram-positives (GP) and 18 fungi. Microorganism identifications in GN were coincident with conventional identification, at the species level, in 83.3% of BC and, at the genus level, in 96.6%. In GP, identifications were coincident with conventional identification in 31.8% of BC at the species level, and in 64.8% at the genus level. Fungaemia was not reliably detected by MALDI-TOF. In 18 BC positive for Candida species (eight C. albicans, nine C. parapsilosis and one C. tropicalis), no microorganisms were identified at the species level, and only one (5.6%) was detected at the genus level. The results of the present study show that this fast, MALDI-TOF MS-based method allows bacterial identification directly from presumptively positive BC in a short time (<30 min), with a high accuracy, especially when GN bacteria are involved. © 2010 The Authors. Clinical Microbiology and Infection © 2010 European Society of Clinical Microbiology and Infectious Diseases.

  10. Control and Non-Payload Communications (CNPC) Prototype Radio - Generation 2 Security Flight Test Report

    Science.gov (United States)

    Iannicca, Dennis C.; Ishac, Joseph A.; Shalkhauser, Kurt A.

    2015-01-01

    NASA Glenn Research Center (GRC), in cooperation with Rockwell Collins, is working to develop a prototype Control and Non-Payload Communications (CNPC) radio platform as part of NASA Integrated Systems Research Program's (ISRP) Unmanned Aircraft Systems (UAS) Integration in the National Airspace System (NAS) project. A primary focus of the project is to work with the Federal Aviation Administration (FAA) and industry standards bodies to build and demonstrate a safe, secure, and efficient CNPC architecture that can be used by industry to evaluate the feasibility of deploying a system using these technologies in an operational capacity. GRC has been working in conjunction with these groups to assess threats, identify security requirements, and to develop a system of standards-based security controls that can be applied to the GRC prototype CNPC architecture as a demonstration platform. The proposed security controls were integrated into the GRC flight test system aboard our S-3B Viking surrogate aircraft and several network tests were conducted during a flight on November 15th, 2014 to determine whether the controls were working properly within the flight environment. The flight test was also the first to integrate Robust Header Compression (ROHC) as a means of reducing the additional overhead introduced by the security controls and Mobile IPv6. The effort demonstrated the complete end-to-end secure CNPC link in a relevant flight environment.

  11. Test Hardware Design for Flight-Like Operation of Advanced Stirling Convertors

    Science.gov (United States)

    Oriti, Salvatore M.

    2012-01-01

    NASA Glenn Research Center (GRC) has been supporting development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG project is providing life, reliability, and performance testing of the Advanced Stirling Convertor (ASC). For this purpose, the Thermal Energy Conversion branch at GRC has been conducting extended operation of a multitude of free-piston Stirling convertors. The goal of this effort is to generate long-term performance data (tens of thousands of hours) simultaneously on multiple units to build a life and reliability database. The test hardware for operation of these convertors was designed to permit in-air investigative testing, such as performance mapping over a range of environmental conditions. With this, there was no requirement to accurately emulate the flight hardware. For the upcoming ASC-E3 units, the decision has been made to assemble the convertors into a flight-like configuration. This means the convertors will be arranged in the dual-opposed configuration in a housing that represents the fit, form, and thermal function of the ASRG. The goal of this effort is to enable system level tests that could not be performed with the traditional test hardware at GRC. This offers the opportunity to perform these system-level tests much earlier in the ASRG flight development, as they would normally not be performed until fabrication of the qualification unit. This paper discusses the requirements, process, and results of this flight-like hardware design activity.

  12. Comparison of Vitek Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Versus Conventional Methods in Candida Identification.

    Science.gov (United States)

    Keçeli, Sema Aşkın; Dündar, Devrim; Tamer, Gülden Sönmez

    2016-02-01

    Candida species are generally identified by conventional methods such as germ tube or morphological appearance on corn meal agar, biochemical methods using API kits and molecular biological methods. Alternative to these methods, rapid and accurate identification methods of microorganisms called matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDİ-TOF MS) has recently been described. In this study, Candida identification results by API Candida kit, API 20C AUX kit and identifications on corn meal agar (CMA) are compared with the results obtained on Vitek-MS. All results were confirmed by sequencing internal transcribed spacer (ITS) regions of rDNA. Totally, 97 Candida strains were identified by germ tube test, CMA, API and Vitek-MS. Vitek-MS results were compatible with 74.2 % of API 20C AUX and 81.4 % of CMA results. The difference between the results of API Candida and API 20C AUX was detected. The ratio of discrepancy between Vitek-MS and API 20C AUX was 25.8 %. Candida species mostly identified as C. famata or C. tropicalis by and not compatible with API kits were identified as C. albicans by Vitek-MS. Sixteen Candida species having discrepant results with Vitek-MS, API or CMA were randomly chosen, and ITS sequence analysis was performed. The results of sequencing were compatible 56.2 % with API 20C AUX, 50 % with CMA and 93.7 % with Vitek-MS. When compared with conventional identification methods, MS results are more reliable and rapid for Candida identification. MS system may be used as routine identification method in clinical microbiology laboratories.

  13. Results from the First Two Flights of the Static Computer Memory Integrity Testing Experiment

    Science.gov (United States)

    Hancock, Thomas M., III

    1999-01-01

    This paper details the scientific objectives, experiment design, data collection method, and post flight analysis following the first two flights of the Static Computer Memory Integrity Testing (SCMIT) experiment. SCMIT is designed to detect soft-event upsets in passive magnetic memory. A soft-event upset is a change in the logic state of active or passive forms of magnetic memory, commonly referred to as a "Bitflip". In its mildest form a soft-event upset can cause software exceptions, unexpected events, start spacecraft safeing (ending data collection) or corrupted fault protection and error recovery capabilities. In it's most severe form loss of mission or spacecraft can occur. Analysis after the first flight (in 1991 during STS-40) identified possible soft-event upsets to 25% of the experiment detectors. Post flight analysis after the second flight (in 1997 on STS-87) failed to find any evidence of soft-event upsets. The SCMIT experiment is currently scheduled for a third flight in December 1999 on STS-101.

  14. The Model Identification Test: A Limited Verbal Science Test

    Science.gov (United States)

    McIntyre, P. J.

    1972-01-01

    Describes the production of a test with a low verbal load for use with elementary school science students. Animated films were used to present appropriate and inappropriate models of the behavior of particles of matter. (AL)

  15. Study of a prototype module of a precision time-of-flight detector for particle identification at low momentum

    CERN Document Server

    AUTHOR|(INSPIRE)INSPIRE-00388630

    In this thesis, Time Of internally Reflected Cherenkov light detector (TORCH), proposed for the LHCb Upgrade to perform three-sigma separation between kaon and pion up to 10$\\ \\rm{GeV}/\\textit{c}$, was studied. TORCH is designed to add significant particle identification capability to the existing LHCb system based on two gas Ring Imaging Cherenkov detectors. TORCH would be placed at $\\sim$ 10 m from the interaction point, where the flight time difference between a primary pion and kaon is 37.5 ps. TORCH will give a pion-kaon separation of three sigma at 10$\\ \\rm{GeV}/\\textit{c}$ from the flight time using the Cherenkov photons generated by the charged particle in a 1 cm-thick quartz plate. In order to calculate accurately the flight time in a busy LHCb environment, Cherenkov angle and photon detection time information, as well as the momentum information from the tracking detector are included in the analysis. For the required TORCH performance, the flight time difference must be measured with a resolution o...

  16. Particle Identification Studies with an ALICE Test TPC

    CERN Document Server

    Christiansen, P

    2007-01-01

    Using a test TPC, consisting of the ALICE TPC field cage prototype in combination with the final ALICE TPC readout and electronics, the energy loss distribution and resolution were measured for identified protons. The measurements were compared to theoretical calculations and good quantitative agreement was found when detector effects were taken into account. The implications for particle identification are discussed.

  17. Reliability Generalization of the Alcohol Use Disorder Identification Test.

    Science.gov (United States)

    Shields, Alan L.; Caruso, John C.

    2002-01-01

    Evaluated the reliability of scores from the Alcohol Use Disorders Identification Test (AUDIT; J. Sounders and others, 1993) in a reliability generalization study based on 17 empirical journal articles. Results show AUDIT scores to be generally reliable for basic assessment. (SLD)

  18. Simple Sample Preparation Method for Direct Microbial Identification and Susceptibility Testing From Positive Blood Cultures.

    Science.gov (United States)

    Pan, Hong-Wei; Li, Wei; Li, Rong-Guo; Li, Yong; Zhang, Yi; Sun, En-Hua

    2018-01-01

    Rapid identification and determination of the antibiotic susceptibility profiles of the infectious agents in patients with bloodstream infections are critical steps in choosing an effective targeted antibiotic for treatment. However, there has been minimal effort focused on developing combined methods for the simultaneous direct identification and antibiotic susceptibility determination of bacteria in positive blood cultures. In this study, we constructed a lysis-centrifugation-wash procedure to prepare a bacterial pellet from positive blood cultures, which can be used directly for identification by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and antibiotic susceptibility testing by the Vitek 2 system. The method was evaluated using a total of 129 clinical bacteria-positive blood cultures. The whole sample preparation process could be completed in identification was 96.49% for gram-negative bacteria and 97.22% for gram-positive bacteria. Vitek 2 antimicrobial susceptibility testing of gram-negative bacteria showed an agreement rate of antimicrobial categories of 96.89% with a minor error, major error, and very major error rate of 2.63, 0.24, and 0.24%, respectively. Category agreement of antimicrobials against gram-positive bacteria was 92.81%, with a minor error, major error, and very major error rate of 4.51, 1.22, and 1.46%, respectively. These results indicated that our direct antibiotic susceptibility analysis method worked well compared to the conventional culture-dependent laboratory method. Overall, this fast, easy, and accurate method can facilitate the direct identification and antibiotic susceptibility testing of bacteria in positive blood cultures.

  19. Simple Sample Preparation Method for Direct Microbial Identification and Susceptibility Testing From Positive Blood Cultures

    Directory of Open Access Journals (Sweden)

    Hong-wei Pan

    2018-03-01

    Full Text Available Rapid identification and determination of the antibiotic susceptibility profiles of the infectious agents in patients with bloodstream infections are critical steps in choosing an effective targeted antibiotic for treatment. However, there has been minimal effort focused on developing combined methods for the simultaneous direct identification and antibiotic susceptibility determination of bacteria in positive blood cultures. In this study, we constructed a lysis-centrifugation-wash procedure to prepare a bacterial pellet from positive blood cultures, which can be used directly for identification by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS and antibiotic susceptibility testing by the Vitek 2 system. The method was evaluated using a total of 129 clinical bacteria-positive blood cultures. The whole sample preparation process could be completed in <15 min. The correct rate of direct MALDI-TOF MS identification was 96.49% for gram-negative bacteria and 97.22% for gram-positive bacteria. Vitek 2 antimicrobial susceptibility testing of gram-negative bacteria showed an agreement rate of antimicrobial categories of 96.89% with a minor error, major error, and very major error rate of 2.63, 0.24, and 0.24%, respectively. Category agreement of antimicrobials against gram-positive bacteria was 92.81%, with a minor error, major error, and very major error rate of 4.51, 1.22, and 1.46%, respectively. These results indicated that our direct antibiotic susceptibility analysis method worked well compared to the conventional culture-dependent laboratory method. Overall, this fast, easy, and accurate method can facilitate the direct identification and antibiotic susceptibility testing of bacteria in positive blood cultures.

  20. Implementation and Test of the Automatic Flight Dynamics Operations for Geostationary Satellite Mission

    Directory of Open Access Journals (Sweden)

    Sangwook Park

    2009-12-01

    Full Text Available This paper describes the Flight Dynamics Automation (FDA system for COMS Flight Dynamics System (FDS and its test result in terms of the performance of the automation jobs. FDA controls the flight dynamics functions such as orbit determination, orbit prediction, event prediction, and fuel accounting. The designed FDA is independent from the specific characteristics which are defined by spacecraft manufacturer or specific satellite missions. Therefore, FDA could easily links its autonomous job control functions to any satellite mission control system with some interface modification. By adding autonomous system along with flight dynamics system, it decreases the operator’s tedious and repeated jobs but increase the usability and reliability of the system. Therefore, FDA is used to improve the completeness of whole mission control system’s quality. The FDA is applied to the real flight dynamics system of a geostationary satellite, COMS and the experimental test is performed. The experimental result shows the stability and reliability of the mission control operations through the automatic job control.

  1. Aerodynamic Parameters of High Performance Aircraft Estimated from Wind Tunnel and Flight Test Data

    Science.gov (United States)

    Klein, Vladislav; Murphy, Patrick C.

    1998-01-01

    A concept of system identification applied to high performance aircraft is introduced followed by a discussion on the identification methodology. Special emphasis is given to model postulation using time invariant and time dependent aerodynamic parameters, model structure determination and parameter estimation using ordinary least squares an mixed estimation methods, At the same time problems of data collinearity detection and its assessment are discussed. These parts of methodology are demonstrated in examples using flight data of the X-29A and X-31A aircraft. In the third example wind tunnel oscillatory data of the F-16XL model are used. A strong dependence of these data on frequency led to the development of models with unsteady aerodynamic terms in the form of indicial functions. The paper is completed by concluding remarks.

  2. Lateral control required for satisfactory flying qualities based on flight tests of numerous airplanes

    Science.gov (United States)

    Gilruth, R R; Turner, W N

    1941-01-01

    Report presents the results of an analysis made of the aileron control characteristics of numerous airplanes tested in flight by the National Advisory Committee for Aeronautics. By the use of previously developed theory, the observed values of pb/2v for the various wing-aileron arrangements were examined to determine the effective section characteristics of the various aileron types.

  3. ASKA STOL research aircraft flight tests and evaluation. STOL jikkenki Asuka'' no hiko shiken kekka

    Energy Technology Data Exchange (ETDEWEB)

    Kuriyama, M; Inoue, T; Tobinaga, Y; Tsuji, H [Kawasaki Heavy Industries, Ltd., Tokyo (Japan)

    1991-07-20

    The present report evaluated the powered high-lift device (PHLD) distance of upper surface blowing (USB) system, basing the materialization of short distance take-off and landing (STOL) performance, one of the main flight test purposes by the Aska'', quiet STOL research aircraft, which evaluation was then added with reporting its flight test result to cover several topics. As prototypical, a C-1 tactical transport aircraft produced by Kawasaki Heavy Industries was modified to the aska'' together with the following change in design for the STOL flight test: Adoption was made of a PHLD of USB system where the wing surface was mounted with four turbofan jet engines thereon. Application was made of a boundary layer control (BLC) to the main wing leading edge and aileron. Mounting was made of a stability and control augmentation system (SCAS) using a triple system digital computer. Fitting was made of a vortex generator for the prevention from peeling by jet exhaust. As a result of flight test, the recorded distance was confirmed to be 1580ft in landing and 1670ft in take-off. 5 refs., 15 figs., 2 tabs.

  4. Aerodynamic and Acoustic Flight Test Results for the Stratospheric Observatory for Infrared Astronomy

    Science.gov (United States)

    Cumming, Stephen B.; Cliatt, Larry James; Frederick, Michael A.; Smith, Mark S.

    2013-01-01

    As part of the Stratospheric Observatory for Infrared Astronomy (SOFIA) program, a 747SP airplane was modified to carry a 2.5 meter telescope in the aft section of the fuselage. The resulting airborne observatory allows for observations above 99 percent of the water vapor in the atmosphere. The open cavity created by the modifications had the potential to significantly affect the airplane in the areas of aerodynamics and acoustics. Several series of flight tests were conducted to clear the airplanes operating envelope for astronomical observations, planned to be performed between the altitudes of 39,000 feet and 45,000 feet. The flight tests were successfully completed. Cavity acoustics were below design limits, and the overall acoustic characteristics of the cavity were better than expected. The modification did have some effects on the stability and control of the airplane, but these effects were not significant. Airplane air data systems were not affected by the modifications. This paper describes the methods used to examine the aerodynamics and acoustic data from the flight tests and provides a discussion of the flight test results in the areas of cavity acoustics, stability and control, and air data.

  5. Aerodynamic and Acoustic Flight Test Results and Results for the Stratospheric Observatory for Infrared Astronomy

    Science.gov (United States)

    Cumming, Stephen B.; Smith, Mark S.; Cliatt, Larry J.; Frederick, Michael A.

    2014-01-01

    As part of the Stratospheric Observatory for Infrared Astronomy program, a 747SP airplane was modified to carry a 2.5-m telescope in the aft section of the fuselage. The resulting airborne observatory allows for observations above 99 percent of the water vapor in the atmosphere. The open cavity created by the modifications had the potential to significantly affect the airplane in the areas of aerodynamics and acoustics. Several series of flight tests were conducted to clear the operating envelope of the airplane for astronomical observations, planned to be performed between the altitudes of 35,000 ft and 45,000 ft. The flight tests were successfully completed. Cavity acoustics were below design limits, and the overall acoustic characteristics of the cavity were better than expected. The modification did have some effects on the stability and control of the airplane, but these effects were not significant. Airplane air data systems were not affected by the modifications. This paper describes the methods used to examine the aerodynamics and acoustic data from the flight tests and provides a discussion of the flight-test results in the areas of cavity acoustics, stability and control, and air data.

  6. Development, test and flight results of the rf systems for the yes2 tether experiment

    NARCIS (Netherlands)

    Cucarella, Guillermina Castillejo; Cichocki, Andrzej; Burla, M.

    2008-01-01

    This paper highlights design, realization, testing and flight results of the Radio Frequency developments (RF) for ESA's second Young Engineers' Satellite (YES2), that included GPS systems, an intersatellite UHF link and a re-entry capsule telemetry and recovery system. The YES2 piggybacked on the

  7. Normobaric hypoxia inhalation test vs. response to airline flight in healthy passengers.

    Science.gov (United States)

    Kelly, Paul T; Swanney, Maureen P; Frampton, Chris; Seccombe, Leigh M; Peters, Matthew J; Beckert, Lutz E

    2006-11-01

    There is little data available to determine the normal response to normobaric hypoxia inhalation testing (NHIT) and air travel. Quantifying a healthy response may assist in the evaluation of passengers considered at risk for air travel. The aims of this study were: (1) to quantify the degree of desaturation in healthy subjects during a NHIT and air travel; and (2) assess the validity of the NHIT when compared with actual in-flight responses. There were 15 healthy adults (age 23-57; 10 women) who volunteered for this study. Preflight tests included lung function, arterial blood gas, pulse oximetry (SpO2), and NHIT (inspired oxygen 15%). SpO2 and cabin pressure were measured continuously on each subject during a commercial air flight (mean cabin altitude 2178 m; range 1719-2426 m). In-flight oxygenation was compared with the preflight NHIT. Lung function testing results were normal. There was significant desaturation (SpO2) during the NHIT (pre: 98 +/- 2%; post: 92 +/- 2%) and at cruising altitude (pre: 97 +/- 1%; cruise: 92 +/- 2%). There was no difference between the final NHIT SpO2 and the mean in-flight SpO2. There was a significant difference between the lowest in-flight SpO2 (88 +/- 2%) vs. the lowest NHIT SpO2, (90 +/- 2%). Oxygen saturation decreases significantly during air travel in normal individuals. In this group of healthy passengers the NHIT approximates some, but not all, aspects of in-flight oxygenation. These results can be used to describe a normal response to the NHIT and air-travel.

  8. The "Sniffin' Kids" test--a 14-item odor identification test for children.

    Directory of Open Access Journals (Sweden)

    Valentin A Schriever

    Full Text Available Tools for measuring olfactory function in adults have been well established. Although studies have shown that olfactory impairment in children may occur as a consequence of a number of diseases or head trauma, until today no consensus on how to evaluate the sense of smell in children exists in Europe. Aim of the study was to develop a modified "Sniffin' Sticks" odor identification test, the "Sniffin' Kids" test for the use in children. In this study 537 children between 6-17 years of age were included. Fourteen odors, which were identified at a high rate by children, were selected from the "Sniffin' Sticks" 16-item odor identification test. Normative date for the 14-item "Sniffin' Kids" odor identification test was obtained. The test was validated by including a group of congenital anosmic children. Results show that the "Sniffin' Kids" test is able to discriminate between normosmia and anosmia with a cutoff value of >7 points on the odor identification test. In addition the test-retest reliability was investigated in a group of 31 healthy children and shown to be ρ = 0.44. With the 14-item odor identification "Sniffin' Kids" test we present a valid and reliable test for measuring olfactory function in children between ages 6-17 years.

  9. Identification and Antimicrobial Susceptibility Testing of Anaerobic Bacteria: Rubik's Cube of Clinical Microbiology?

    Science.gov (United States)

    Gajdács, Márió; Spengler, Gabriella; Urbán, Edit

    2017-11-07

    Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically. In the past, antimicrobial susceptibility of these microorganisms showed predictable patterns and empirical therapy could be safely administered but recently a steady and clear increase in the resistance for several important drugs (β-lactams, clindamycin) has been observed worldwide. For this reason, antimicrobial susceptibility testing of anaerobic isolates for surveillance purposes or otherwise is of paramount importance but the availability of these testing methods is usually limited. In this present review, our aim was to give an overview of the methods currently available for the identification (using phenotypic characteristics, biochemical testing, gas-liquid chromatography, MALDI-TOF MS and WGS) and antimicrobial susceptibility testing (agar dilution, broth microdilution, disk diffusion, gradient tests, automated systems, phenotypic and molecular resistance detection techniques) of anaerobes, when should these methods be used and what are the recent developments in resistance patterns of anaerobic bacteria.

  10. Identification and Antimicrobial Susceptibility Testing of Anaerobic Bacteria: Rubik’s Cube of Clinical Microbiology?

    Science.gov (United States)

    Gajdács, Márió; Spengler, Gabriella; Urbán, Edit

    2017-01-01

    Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically. In the past, antimicrobial susceptibility of these microorganisms showed predictable patterns and empirical therapy could be safely administered but recently a steady and clear increase in the resistance for several important drugs (β-lactams, clindamycin) has been observed worldwide. For this reason, antimicrobial susceptibility testing of anaerobic isolates for surveillance purposes or otherwise is of paramount importance but the availability of these testing methods is usually limited. In this present review, our aim was to give an overview of the methods currently available for the identification (using phenotypic characteristics, biochemical testing, gas-liquid chromatography, MALDI-TOF MS and WGS) and antimicrobial susceptibility testing (agar dilution, broth microdilution, disk diffusion, gradient tests, automated systems, phenotypic and molecular resistance detection techniques) of anaerobes, when should these methods be used and what are the recent developments in resistance patterns of anaerobic bacteria. PMID:29112122

  11. Shuttle Return-to-Flight IH-108 Aerothermal Test at CUBRC - Flow Field Calibration and CFD

    Science.gov (United States)

    Lau, Kei Y.; Holden, M. S.

    2011-01-01

    This paper discusses one specific aspect of the Shuttle Retrun-To-Flight IH-108 Aerothermal Test at Calspan-University of Buffalo Research Center (CUBRC), the test flow field calibration. It showed the versatility of the CUBRC Large Energy National Shock Tunnel (LENS) II wind tunnel for an aerothermal test with unique and demanding requirements. CFD analyses were used effectively to extend the test range at the low end of the Mach range. It demonstrated how ground test facility and CFD synergy can be utilitzed iteratively to enhance the confidence in the fedility of both tools. It addressed the lingering concerns of the aerothermal community on use of inpulse facility and CFD analysis. At the conclusion of the test program, members from the NASA Marshall (MSFC), CUBRC and USA (United Space Alliance) Consultants (The Grey Beards) were asked to independently verify the flight scaling data generated by Boeing for flight certification of the re-designed external tank (ET) components. The blind test comparison showed very good results.

  12. Development of Flight-Test Performance Estimation Techniques for Small Unmanned Aerial Systems

    Science.gov (United States)

    McCrink, Matthew Henry

    This dissertation provides a flight-testing framework for assessing the performance of fixed-wing, small-scale unmanned aerial systems (sUAS) by leveraging sub-system models of components unique to these vehicles. The development of the sub-system models, and their links to broader impacts on sUAS performance, is the key contribution of this work. The sub-system modeling and analysis focuses on the vehicle's propulsion, navigation and guidance, and airframe components. Quantification of the uncertainty in the vehicle's power available and control states is essential for assessing the validity of both the methods and results obtained from flight-tests. Therefore, detailed propulsion and navigation system analyses are presented to validate the flight testing methodology. Propulsion system analysis required the development of an analytic model of the propeller in order to predict the power available over a range of flight conditions. The model is based on the blade element momentum (BEM) method. Additional corrections are added to the basic model in order to capture the Reynolds-dependent scale effects unique to sUAS. The model was experimentally validated using a ground based testing apparatus. The BEM predictions and experimental analysis allow for a parameterized model relating the electrical power, measurable during flight, to the power available required for vehicle performance analysis. Navigation system details are presented with a specific focus on the sensors used for state estimation, and the resulting uncertainty in vehicle state. Uncertainty quantification is provided by detailed calibration techniques validated using quasi-static and hardware-in-the-loop (HIL) ground based testing. The HIL methods introduced use a soft real-time flight simulator to provide inertial quality data for assessing overall system performance. Using this tool, the uncertainty in vehicle state estimation based on a range of sensors, and vehicle operational environments is

  13. Guidelines of the Design of Electropyrotechnic Firing Circuit for Unmanned Flight and Ground Test Projects

    Science.gov (United States)

    Gonzalez, Guillermo A.; Lucy, Melvin H.; Massie, Jeffrey J.

    2013-01-01

    The NASA Langley Research Center, Engineering Directorate, Electronic System Branch, is responsible for providing pyrotechnic support capabilities to Langley Research Center unmanned flight and ground test projects. These capabilities include device selection, procurement, testing, problem solving, firing system design, fabrication and testing; ground support equipment design, fabrication and testing; checkout procedures and procedure?s training to pyro technicians. This technical memorandum will serve as a guideline for the design, fabrication and testing of electropyrotechnic firing systems. The guidelines will discuss the entire process beginning with requirements definition and ending with development and execution.

  14. Research on computer aided testing of pilot response to critical in-flight events

    Science.gov (United States)

    Giffin, W. C.; Rockwell, T. H.; Smith, P. J.

    1984-01-01

    Experiments on pilot decision making are described. The development of models of pilot decision making in critical in flight events (CIFE) are emphasized. The following tests are reported on the development of: (1) a frame system representation describing how pilots use their knowledge in a fault diagnosis task; (2) assessment of script norms, distance measures, and Markov models developed from computer aided testing (CAT) data; and (3) performance ranking of subject data. It is demonstrated that interactive computer aided testing either by touch CRT's or personal computers is a useful research and training device for measuring pilot information management in diagnosing system failures in simulated flight situations. Performance is dictated by knowledge of aircraft sybsystems, initial pilot structuring of the failure symptoms and efficient testing of plausible causal hypotheses.

  15. Threshold Assessment of Gear Diagnostic Tools on Flight and Test Rig Data

    Science.gov (United States)

    Dempsey, Paula J.; Mosher, Marianne; Huff, Edward M.

    2003-01-01

    A method for defining thresholds for vibration-based algorithms that provides the minimum number of false alarms while maintaining sensitivity to gear damage was developed. This analysis focused on two vibration based gear damage detection algorithms, FM4 and MSA. This method was developed using vibration data collected during surface fatigue tests performed in a spur gearbox rig. The thresholds were defined based on damage progression during tests with damage. The thresholds false alarm rates were then evaluated on spur gear tests without damage. Next, the same thresholds were applied to flight data from an OH-58 helicopter transmission. Results showed that thresholds defined in test rigs can be used to define thresholds in flight to correctly classify the transmission operation as normal.

  16. Reliable and reproducible method for rapid identification of Nocardia species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Toyokawa, Masahiro; Kimura, Keigo; Nishi, Isao; Sunada, Atsuko; Ueda, Akiko; Sakata, Tomomi; Asari, Seishi

    2013-01-01

    Recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been challenged for the identification of Nocardia species. However, the standard ethanol-formic acid extraction alone is insufficient in allowing the membrane proteins of Nocardia species to be ionized by the matrix. We therefore aimed to establish our new extraction method for the MALDI-TOF MS-based identification of Nocardia species isolates. Our modified extraction procedure is through dissociation in 0.5% Tween-20 followed by bacterial heat-inactivation, mechanical breaking of the cell wall by acid-washed glass beads and protein extraction with formic acid and acetonitrile. As reference methods for species identification, full-length 16S rRNA gene sequencing and some phenotypical tests were used. In a first step, we made our own Nocardia database by analyzing 13 strains (13 different species including N. elegans, N. otitidiscaviarum, N. asiatica, N. abscessus, N. brasiliensis, N. thailandica, N. farcinica, N. nova, N. mikamii, N. cyriacigeorgica, N. asteroids, Nocardiopsis alba, and Micromonospora sp.) and registered to the MALDI BioTyper database. Then we established our database. The analysis of 12 challenge strains using the our database gave a 100% correct identification, including 8 strains identified to the species level and 4 strains to the genus level (N. elegans, N. nova, N. farcinica, Micromonospora sp.) according to the manufacture's log score specifications. In the estimation of reproducibility of our method intended for 4 strains, both within-run and between-run reproducibility were excellent. These data indicates that our method for rapid identification of Nocardia species is with reliability, reproducibility and cost effective.

  17. Pitch control margin at high angle of attack - Quantitative requirements (flight test correlation with simulation predictions)

    Science.gov (United States)

    Lackey, J.; Hadfield, C.

    1992-01-01

    Recent mishaps and incidents on Class IV aircraft have shown a need for establishing quantitative longitudinal high angle of attack (AOA) pitch control margin design guidelines for future aircraft. NASA Langley Research Center has conducted a series of simulation tests to define these design guidelines. Flight test results have confirmed the simulation studies in that pilot rating of high AOA nose-down recoveries were based on the short-term response interval in the forms of pitch acceleration and rate.

  18. SEXTANT X-Ray Pulsar Navigation Demonstration: Flight System and Test Results

    Science.gov (United States)

    Winternitz, Luke; Mitchell, Jason W.; Hassouneh, Munther A.; Valdez, Jennifer E.; Price, Samuel R.; Semper, Sean R.; Yu, Wayne H.; Ray, Paul S.; Wood, Kent S.; Arzoumanian, Zaven; hide

    2016-01-01

    The Station Explorer for X-ray Timing and Navigation Technology (SEXTANT) is a technology demonstration enhancement to the Neutron-star Interior Composition Explorer (NICER) mission. NICER is a NASA Explorer Mission of Opportunity that will be hosted on the International Space Station (ISS). SEXTANT will, for the first time, demonstrate real-time, on-board X-ray Pulsar Navigation (XNAV), a significant milestone in the quest to establish a GPS-like navigation capability available throughout our Solar System and beyond. This paper gives an overview of the SEXTANT system architecture and describes progress prior to environmental testing of the NICER flight instrument. It provides descriptions and development status of the SEXTANT flight software and ground system, as well as detailed description and results from the flight software functional and performance testing within the high-fidelity Goddard Space Flight Center (GSFC) X-ray Navigation Laboratory Testbed (GXLT) software and hardware simulation environment. Hardware-in-the-loop simulation results are presented, using the engineering model of the NICER timing electronics and the GXLT pulsar simulator-the GXLT precisely controls NASA GSFC's unique Modulated X-ray Source to produce X-rays that make the NICER detector electronics appear as if they were aboard the ISS viewing a sequence of millisecond pulsars

  19. Flight Test Experience With an Electromechanical Actuator on the F-18 Systems Research Aircraft

    Science.gov (United States)

    Jensen, Stephen C.; Jenney, Gavin D.; Raymond, Bruce; Dawson, David

    2000-01-01

    Development of reliable power-by-wire actuation systems for both aeronautical and space applications has been sought recently to eliminate hydraulic systems from aircraft and spacecraft and thus improve safety, efficiency, reliability, and maintainability. The Electrically Powered Actuation Design (EPAD) program was a joint effort between the Air Force, Navy, and NASA to develop and fly a series of actuators validating power-by-wire actuation technology on a primary flight control surface of a tactical aircraft. To achieve this goal, each of the EPAD actuators was installed in place of the standard hydraulic actuator on the left aileron of the NASA F/A-18B Systems Research Aircraft (SRA) and flown throughout the SRA flight envelope. Numerous parameters were recorded, and overall actuator performance was compared with the performance of the standard hydraulic actuator on the opposite wing. This paper discusses the integration and testing of the EPAD electromechanical actuator (EMA) on the SRA. The architecture of the EMA system is discussed, as well as its integration with the F/A-18 Flight Control System. The flight test program is described, and actuator performance is shown to be very close to that of the standard hydraulic actuator it replaced. Lessons learned during this program are presented and discussed, as well as suggestions for future research.

  20. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species.

    Science.gov (United States)

    Stevenson, Lindsay G; Drake, Steven K; Shea, Yvonne R; Zelazny, Adrian M; Murray, Patrick R

    2010-10-01

    We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of yeast species. Using Bruker Daltonics MALDI BioTyper software, we created a spectral database library with m/z ratios of 2,000 to 20,000 Da for 109 type and reference strains of yeast (44 species in 8 genera). The database was tested for accuracy by use of 194 clinical isolates (23 species in 6 genera). A total of 192 (99.0%) of the clinical isolates were identified accurately by MALDI-TOF MS. The MALDI-TOF MS-based method was found to be reproducible and accurate, with low consumable costs and minimal preparation time.

  1. Space Station Environmental Control and Life Support System Test Facility at Marshall Space Flight Center

    Science.gov (United States)

    Springer, Darlene

    1989-01-01

    Different aspects of Space Station Environmental Control and Life Support System (ECLSS) testing are currently taking place at Marshall Space Flight Center (MSFC). Unique to this testing is the variety of test areas and the fact that all are located in one building. The north high bay of building 4755, the Core Module Integration Facility (CMIF), contains the following test areas: the Subsystem Test Area, the Comparative Test Area, the Process Material Management System (PMMS), the Core Module Simulator (CMS), the End-use Equipment Facility (EEF), and the Pre-development Operational System Test (POST) Area. This paper addresses the facility that supports these test areas and briefly describes the testing in each area. Future plans for the building and Space Station module configurations will also be discussed.

  2. Automatic Identification System (AIS) Transmit Testing in Louisville Phase 2

    Science.gov (United States)

    2014-08-01

    Firewall Louisville QM 65.206.28.x NAIS Site Controller PC RS232 Serial cable TV32 Computer Cmd Center Serial splitter SAAB R40 AIS Base Station...172.17.14.6 Rack mount computer AIS Radio Interface Ethernet Switch 192.168.0.x Firewall Cable Modem 192.168.0.1 VTS Accred. Boundary serial connection...Automatic Identification System ( AIS ) Transmit Testing in Louisville Phase 2 Distribution Statement A: Approved for public release

  3. Implementation and flight-test of a multi-mode rotorcraft flight-control system for single-pilot use in poor visibility

    Science.gov (United States)

    Hindson, William S.

    1987-01-01

    A flight investigation was conducted to evaluate a multi-mode flight control system designed according to the most recent recommendations for handling qualities criteria for new military helicopters. The modes and capabilities that were included in the system are those considered necessary to permit divided-attention (single-pilot) lowspeed and hover operations near the ground in poor visibility conditions. Design features included mode-selection and mode-blending logic, the use of an automatic position-hold mode that employed precision measurements of aircraft position, and a hover display which permitted manually-controlled hover flight tasks in simulated instrument conditions. Pilot evaluations of the system were conducted using a multi-segment evaluation task. Pilot comments concerning the use of the system are provided, and flight-test data are presented to show system performance.

  4. Flight Extraction and Phase Identification for Large Automatic Dependent Surveillance–Broadcast Datasets

    NARCIS (Netherlands)

    Sun, J.; Ellerbroek, J.; Hoekstra, J.M.

    2017-01-01

    AUTOMATIC dependent surveillance–broadcast (ADS-B) [1,2] is widely implemented in modern commercial aircraft and will become mandatory equipment in 2020. Flight state information such as position, velocity, and vertical rate are broadcast by tens of thousands of aircraft around the world constantly

  5. Large-Scale Flight Phase Identification from ADS-B Data Using Machine Learning Methods

    NARCIS (Netherlands)

    Sun, J.; Ellerbroek, J.; Hoekstra, J.M.; Lovell, D.; Fricke, H.

    2016-01-01

    With the increasing availability of ADS-B transponders on commercial aircraft, as well as the rapidly growing deployment of ground stations that provide public access to their data, accessing open aircraft flight data is becoming easier for researchers. Given the large number of operational

  6. Closed-Loop System Identification Experience for Flight Control Law and Flying Qualities Evaluation of a High Performance Fighter Aircraft

    Science.gov (United States)

    Murphy, Patrick C.

    1999-01-01

    This paper highlights some of the results and issues associated with estimating models to evaluate control law design methods and design criteria for advanced high performance aircraft. Experimental fighter aircraft such as the NASA High Alpha Research Vehicle (HARV) have the capability to maneuver at very high angles of attack where nonlinear aerodynamics often predominate. HARV is an experimental F/A-18, configured with thrust vectoring and conformal actuated nose strakes. Identifying closed-loop models for this type of aircraft can be made difficult by nonlinearities and high-order characteristics of the system. In this paper only lateral-directional axes are considered since the lateral-directional control law was specifically designed to produce classical airplane responses normally expected with low-order, rigid-body systems. Evaluation of the control design methodology was made using low-order equivalent systems determined from flight and simulation. This allowed comparison of the closed-loop rigid-body dynamics achieved in flight with that designed in simulation. In flight, the On Board Excitation System was used to apply optimal inputs to lateral stick and pedals at five angles of attack: 5, 20, 30, 45, and 60 degrees. Data analysis and closed-loop model identification were done using frequency domain maximum likelihood. The structure of the identified models was a linear state-space model reflecting classical 4th-order airplane dynamics. Input time delays associated with the high-order controller and aircraft system were accounted for in data preprocessing. A comparison of flight estimated models with small perturbation linear design models highlighted nonlinearities in the system and indicated that the estimated closed-loop rigid-body dynamics were sensitive to input amplitudes at 20 and 30 degrees angle of attack.

  7. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens.

    Science.gov (United States)

    Huang, Yanfei; Wang, Jinglin; Zhang, Mingxin; Zhu, Min; Wang, Mei; Sun, Yufeng; Gu, Haitong; Cao, Jingjing; Li, Xue; Zhang, Shaoya; Lu, Xinxin

    2017-03-01

    Filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. Although matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS has been employed to identify various fungi, its efficacy in the identification of FRS fungi is less clear. A total of 153 FRS isolates obtained from patients were analysed at the Clinical Laboratory at the Beijing Tongren Hospital affiliated to the Capital Medical University, between January 2014 and December 2015. They were identified by traditional phenotypic methods and Bruker MALDI-TOF MS (Bruker, Biotyper version 3.1), respectively. Discrepancies between the two methods were further validated by sequencing. Among the 153 isolates, 151 had correct species identification using MALDI-TOF MS (Bruker, Biot 3.1, score ≥2.0 or 2.3). MALDI-TOF MS enabled identification of some very closely related species that were indistinguishable by conventional phenotypic methods, including 1/10 Aspergillus versicolor, 3/20 Aspergillus flavus, 2/30 Aspergillus fumigatus and 1/20 Aspergillus terreus, which were misidentified by conventional phenotypic methods as Aspergillus nidulans, Aspergillus oryzae, Aspergillus japonicus and Aspergillus nidulans, respectively. In addition, 2/2 Rhizopus oryzae and 1/1 Rhizopus stolonifer that were identified only to the genus level by the phenotypic method were correctly identified by MALDI-TOF MS. MALDI-TOF MS is a rapid and accurate technique, and could replace the conventional phenotypic method for routine identification of FRS fungi in clinical microbiology laboratories.

  8. Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems

    DEFF Research Database (Denmark)

    Justesen, Ulrik Stenz; Holm, Anette; Knudsen, Elisa

    2011-01-01

    We compared two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level...... (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important...

  9. A Description of the Software Element of the NASA EME Flight Tests

    Science.gov (United States)

    Koppen, Sandra V.

    1996-01-01

    In support of NASA's Fly-By-Light/Power-By-Wire (FBL/PBW) program, a series of flight tests were conducted by NASA Langley Research Center in February, 1995. The NASA Boeing 757 was flown past known RF transmitters to measure both external and internal radiated fields. The aircraft was instrumented with strategically located sensors for acquiring data on shielding effectiveness and internal coupling. The data are intended to support computational and statistical modeling codes used to predict internal field levels of an electromagnetic environment (EME) on aircraft. The software was an integral part of the flight tests, as well as the data reduction process. The software, which provided flight test instrument control, data acquisition, and a user interface, executes on a Hewlett Packard (HP) 300 series workstation and uses BP VEEtest development software and the C programming language. Software tools were developed for data processing and analysis, and to provide a database organized by frequency bands, test runs, and sensors. This paper describes the data acquisition system on board the aircraft and concentrates on the software portion. Hardware and software interfaces are illustrated and discussed. Particular attention is given to data acquisition and data format. The data reduction process is discussed in detail to provide insight into the characteristics, quality, and limitations of the data. An analysis of obstacles encountered during the data reduction process is presented.

  10. Modeling and Closed Loop Flight Testing of a Fixed Wing Micro Air Vehicle

    Directory of Open Access Journals (Sweden)

    Harikumar Kandath

    2018-03-01

    Full Text Available This paper presents the nonlinear six degrees of freedom dynamic modeling of a fixed wing micro air vehicle. The static derivatives of the micro air vehicle are obtained through the wind tunnel testing. The propeller effects on the lift, drag, pitching moment and side force are quantified through wind tunnel testing. The dynamic derivatives are obtained through empirical relations available in the literature. The trim conditions are computed for a straight and constant altitude flight condition. The linearized longitudinal and lateral state space models are obtained about trim conditions. The variations in short period mode, phugoid mode, Dutch roll mode, roll subsidence mode and spiral mode with respect to different trim operating conditions is presented. A stabilizing static output feedback controller is designed using the obtained model. Successful closed loop flight trials are conducted with the static output feedback controller.

  11. Flight Flutter Testing of Rotary Wing Aircraft Using a Control System Oscillation Technique

    Science.gov (United States)

    Yen, J. G.; Viswanathan, S.; Matthys, C. G.

    1976-01-01

    A flight flutter testing technique is described in which the rotor controls are oscillated by series actuators to excite the rotor and airframe modes of interest, which are then allowed to decay. The moving block technique is then used to determine the damped frequency and damping variation with rotor speed. The method proved useful for tracking the stability of relatively well damped modes. The results of recently completed flight tests of an experimental soft-in-plane rotor are used to illustrate the technique. Included is a discussion of the application of this technique to investigation of the propeller whirl flutter stability characteristics of the NASA/Army XV-15 VTOL tilt rotor research aircraft.

  12. Identification of Candida species isolated from vulvovaginitis using matrix assisted laser desorption ionization-time of flight mass spectrometry.

    Science.gov (United States)

    Alizadeh, Majid; Kolecka, Anna; Boekhout, Teun; Zarrinfar, Hossein; Ghanbari Nahzag, Mohamad A; Badiee, Parisa; Rezaei-Matehkolaei, Ali; Fata, Abdolmajid; Dolatabadi, Somayeh; Najafzadeh, Mohammad J

    2017-12-01

    Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify Candida isolates by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) from women with vulvovaginitis that were referred to Ghaem Hospital, Mashhad, Iran. This study was conducted on 65 clinical samples isolated from women that were referred to Ghaem Hospital. All specimens were identified using phenotyping techniques, such as microscopy and culture on Sabouraud dextrose agar and corn meal agar. In addition, all isolates were processed for MALDI-TOF MS identification. Out of the 65 analyzed isolates, 61 (94%) samples were recognized by MALDI-TOF MS. However, the remaining four isolates (6%) had no reliable identification. According to the results, C. albicans (58.5%) was the most frequently isolated species, followed by C. tropicalis (16.9%), C. glabrata (7.7%), C. parapsilosis (7.7%), and guilliermondii (3.1%). As the findings indicated, MALDI TOF MS was successful in the identification of clinical Candida species. C. albicans was identified as the most common Candida species isolated from the women with VVC. Moreover, C. tropicalis was the most common species among the non- albicans Candida species.

  13. Orion Exploration Flight Test 1 (EFT-1) Best Estimated Trajectory Development

    Science.gov (United States)

    Holt, Greg N.; Brown, Aaron

    2016-01-01

    The Orion Exploration Flight Test 1 (EFT-1) mission successfully flew on Dec 5, 2014 atop a Delta IV Heavy launch vehicle. The goal of Orions maiden flight was to stress the system by placing an uncrewed vehicle on a high-energy trajectory replicating conditions similar to those that would be experienced when returning from an asteroid or a lunar mission. The Orion navigation team combined all trajectory data from the mission into a Best Estimated Trajectory (BET) product. There were significant challenges in data reconstruction and many lessons were learned for future missions. The team used an estimation filter incorporating radar tracking, onboard sensors (Global Positioning System and Inertial Measurement Unit), and day-of-flight weather balloons to evaluate the true trajectory flown by Orion. Data was published for the entire Orion EFT-1 flight, plus objects jettisoned during entry such as the Forward Bay Cover. The BET customers include approximately 20 disciplines within Orion who will use the information for evaluating vehicle performance and influencing future design decisions.

  14. Flight-Test Evaluation of Kinematic Precise Point Positioning of Small UAVs

    Directory of Open Access Journals (Sweden)

    Jason N. Gross

    2016-01-01

    Full Text Available An experimental analysis of Global Positioning System (GPS flight data collected onboard a Small Unmanned Aerial Vehicle (SUAV is conducted in order to demonstrate that postprocessed kinematic Precise Point Positioning (PPP solutions with precisions approximately 6 cm 3D Residual Sum of Squares (RSOS can be obtained on SUAVs that have short duration flights with limited observational periods (i.e., only ~≤5 minutes of data. This is a significant result for the UAV flight testing community because an important and relevant benefit of the PPP technique over traditional Differential GPS (DGPS techniques, such as Real-Time Kinematic (RTK, is that there is no requirement for maintaining a short baseline separation to a differential GNSS reference station. Because SUAVs are an attractive platform for applications such as aerial surveying, precision agriculture, and remote sensing, this paper offers an experimental evaluation of kinematic PPP estimation strategies using SUAV platform data. In particular, an analysis is presented in which the position solutions that are obtained from postprocessing recorded UAV flight data with various PPP software and strategies are compared to solutions that were obtained using traditional double-differenced ambiguity fixed carrier-phase Differential GPS (CP-DGPS. This offers valuable insight to assist designers of SUAV navigation systems whose applications require precise positioning.

  15. Full-scale flight tests of aircraft morphing structures using SMA actuators

    Science.gov (United States)

    Mabe, James H.; Calkins, Frederick T.; Ruggeri, Robert T.

    2007-04-01

    In August of 2005 The Boeing Company conducted a full-scale flight test utilizing Shape Memory Alloy (SMA) actuators to morph an engine's fan exhaust to correlate exhaust geometry with jet noise reduction. The test was conducted on a 777-300ER with GE-115B engines. The presence of chevrons, serrated aerodynamic surfaces mounted at the trailing edge of the thrust reverser, have been shown to greatly reduce jet noise by encouraging advantageous mixing of the free, and fan streams. The morphing, or Variable Geometry Chevrons (VGC), utilized compact, light weight, and robust SMA actuators to morph the chevron shape to optimize the noise reduction or meet acoustic test objectives. The VGC system was designed for two modes of operation. The entirely autonomous operation utilized changes in the ambient temperature from take-off to cruise to activate the chevron shape change. It required no internal heaters, wiring, control system, or sensing. By design this provided one tip immersion at the warmer take-off temperatures to reduce community noise and another during the cooler cruise state for more efficient engine operation, i.e. reduced specific fuel consumption. For the flight tests a powered mode was added where internal heaters were used to individually control the VGC temperatures. This enabled us to vary the immersions and test a variety of chevron configurations. The flight test demonstrated the value of SMA actuators to solve a real world aerospace problem, validated that the technology could be safely integrated into the airplane's structure and flight system, and represented a large step forward in the realization of SMA actuators for production applications. In this paper the authors describe the development of the actuator system, the steps required to integrate the morphing structure into the thrust reverser, and the analysis and testing that was required to gain approval for flight. Issues related to material strength, thermal environment, vibration

  16. Use of a Commercially Available Flight Simulator during Aircrew Performance Testing.

    Science.gov (United States)

    1991-11-01

    Automiated Battery of Performance-based Tests, NAMRL 1354, Naval Aerospace Medical Research Laboratory, Pensacola, FL, 1990. 13. Human Performance...ability of an aircraft to remain airborne well beyond the limits of its human operator. This capacity for longer flights, coupled with a tendency for short...Measurement, Final Report, Air Force Human Resources Laboratory, Brooks AFB, TX, 1983. 5. Stein, E.S., Measurement of Pilot Performance: A Master Journeyman

  17. Laminar and Turbulent Flow Calculations for the Hifire-5B Flight Test

    Science.gov (United States)

    2017-11-01

    STATES AIR FORCE AFRL-RQ-WP-TP-2017-0172 LAMINAR AND TURBULENT FLOW CALCULATIONS FOR THE HIFIRE-5B FLIGHT TEST Roger L. Kimmel Hypersonic Sciences...stationary instabilities of the three-dimensional flow as the grid becomes finer. It may not be possible to obtain a strictly laminar basic state on a very...fine grid. A basic state solution was desired for the laminar flow calculations, and the oscillations observed in Fig. 3 were judged to be undesirable

  18. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species.

    Science.gov (United States)

    Alanio, A; Beretti, J-L; Dauphin, B; Mellado, E; Quesne, G; Lacroix, C; Amara, A; Berche, P; Nassif, X; Bougnoux, M-E

    2011-05-01

    New Aspergillus species have recently been described with the use of multilocus sequencing in refractory cases of invasive aspergillosis. The classical phenotypic identification methods routinely used in clinical laboratories failed to identify them adequately. Some of these Aspergillus species have specific patterns of susceptibility to antifungal agents, and misidentification may lead to inappropriate therapy. We developed a matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS)-based strategy to adequately identify Aspergillus species to the species level. A database including the reference spectra of 28 clinically relevant species from seven Aspergillus sections (five common and 23 unusual species) was engineered. The profiles of young and mature colonies were analysed for each reference strain, and species-specific spectral fingerprints were identified. The performance of the database was then tested on 124 clinical and 16 environmental isolates previously characterized by partial sequencing of the β-tubulin and calmodulin genes. One hundred and thirty-eight isolates of 140 (98.6%) were correctly identified. Two atypical isolates could not be identified, but no isolate was misidentified (specificity: 100%). The database, including species-specific spectral fingerprints of young and mature colonies of the reference strains, allowed identification regardless of the maturity of the clinical isolate. These results indicate that MALDI-TOF MS is a powerful tool for rapid and accurate identification of both common and unusual species of Aspergillus. It can give better results than morphological identification in clinical laboratories. © 2010 The Authors. Clinical Microbiology and Infection © 2010 European Society of Clinical Microbiology and Infectious Diseases.

  19. Particle identification by time-of-flight measurement in the SAPHIR

    International Nuclear Information System (INIS)

    Hoffmann-Rothe, P.

    1993-02-01

    Using photoproduction data which have been measured with the SAPHIR-detector with different target materials (C H 2 solid , H 2 liquid , D 2 liquid ) a detailed investigation and discussion of the detectors performance to measure the time of flight of charged particles and to separate between particles of different mass has been accomplished. A FORTRAN program has been written which provides a calibration of the scintillator panels of the TOF hodoscopes, calculates correction factors for the time-walk effect an finally, by combining the time of flight with track momentum measurement, determines particle masses. The current configuration of the detector makes it possible to separate between proton and pion up to a particle momentum of 1.6 GeV/c. Proton and kaon can be separated up to a momentum of 1.3 GeV/c, kaon and pion up to a momentum of 0.85 GeV/c. (prog.) [de

  20. Identification of microorganisms using superconducting tunnel junctions and time-of-flight mass spectrometry

    Science.gov (United States)

    Ullom, J. N.; Frank, M.; Horn, J. M.; Labov, S. E.; Langry, K.; Benner, W. H.

    2000-04-01

    We present time-of-flight measurements of biological material ejected from bacterial spores following laser irradiation. Ion impacts are registered on a microchannel plate detector and on a Superconducting Tunnel Junction (STJ) detector. We compare mass spectra obtained with the two detectors. The STJ has better sensitivity to massive ions and also measures the energy of each ion. We show evidence that spores of different bacillus species produce distinctive mass spectra and associate the observed mass peaks with coat proteins.

  1. Identification of microorganisms using superconducting tunnel junctions and time-of-flight mass spectrometry

    International Nuclear Information System (INIS)

    Ullom, J.N.; Frank, M.; Horn, J.M.; Labov, S.E.; Langry, K.; Benner, W.H.

    2000-01-01

    We present time-of-flight measurements of biological material ejected from bacterial spores following laser irradiation. Ion impacts are registered on a microchannel plate detector and on a Superconducting Tunnel Junction (STJ) detector. We compare mass spectra obtained with the two detectors. The STJ has better sensitivity to massive ions and also measures the energy of each ion. We show evidence that spores of different bacillus species produce distinctive mass spectra and associate the observed mass peaks with coat proteins

  2. Flight Test Results from the NF-15B Intelligent Flight Control System (IFCS) Project with Adaptation to a Simulated Stabilator Failure

    Science.gov (United States)

    Bosworth, John T.; Williams-Hayes, Peggy S.

    2010-01-01

    Adaptive flight control systems have the potential to be more resilient to extreme changes in airplane behavior. Extreme changes could be a result of a system failure or of damage to the airplane. A direct adaptive neural-network-based flight control system was developed for the National Aeronautics and Space Administration NF-15B Intelligent Flight Control System airplane and subjected to an inflight simulation of a failed (frozen) (unmovable) stabilator. Formation flight handling qualities evaluations were performed with and without neural network adaptation. The results of these flight tests are presented. Comparison with simulation predictions and analysis of the performance of the adaptation system are discussed. The performance of the adaptation system is assessed in terms of its ability to decouple the roll and pitch response and reestablish good onboard model tracking. Flight evaluation with the simulated stabilator failure and adaptation engaged showed that there was generally improvement in the pitch response; however, a tendency for roll pilot-induced oscillation was experienced. A detailed discussion of the cause of the mixed results is presented.

  3. Germination test for identification of gamma-irradiated bean seeds

    International Nuclear Information System (INIS)

    Wesolowska, B.; Ignatowicz, S.

    1993-01-01

    The feasibility of germination test for the practical detection of irradiated beans has not been investigated. The objective of this study was to determine if the relationship between the root growth rate and radiation dose could be used to produce a rapid analytical method for identification of irradiated beans. Such detection method could be potentially used for both (a) identification of irradiated food, and (b) for quarantine inspection (to certify that the agricultural product has been irradiated, and the pests present in it do not pose a quarantine risk). Results presented in this paper indicate that the germination test is not always capable of discriminating satisfactorily between irradiated and unirradiated samples of bean seeds, because the sensitivity of the test is often higher than the low doses which are suggested for disinfestation purposes. However, using the germination test, an unexperienced person can easily discriminate untreated bean seeds from those irradiated with 0.3-1.5 kGy doses of gamma radiation. (orig./vhe)

  4. Pescara benchmark: overview of modelling, testing and identification

    International Nuclear Information System (INIS)

    Bellino, A; Garibaldi, L; Marchesiello, S; Brancaleoni, F; Gabriele, S; Spina, D; Bregant, L; Carminelli, A; Catania, G; Sorrentino, S; Di Evangelista, A; Valente, C; Zuccarino, L

    2011-01-01

    The 'Pescara benchmark' is part of the national research project 'BriViDi' (BRIdge VIbrations and DIagnosis) supported by the Italian Ministero dell'Universita e Ricerca. The project is aimed at developing an integrated methodology for the structural health evaluation of railway r/c, p/c bridges. The methodology should provide for applicability in operating conditions, easy data acquisition through common industrial instrumentation, robustness and reliability against structural and environmental uncertainties. The Pescara benchmark consisted in lab tests to get a consistent and large experimental data base and subsequent data processing. Special tests were devised to simulate the train transit effects in actual field conditions. Prestressed concrete beams of current industrial production both sound and damaged at various severity corrosion levels were tested. The results were collected either in a deterministic setting and in a form suitable to deal with experimental uncertainties. Damage identification was split in two approaches: with or without a reference model. In the first case f.e. models were used in conjunction with non conventional updating techniques. In the second case, specialized output-only identification techniques capable to deal with time-variant and possibly non linear systems were developed. The lab tests allowed validating the above approaches and the performances of classical modal based damage indicators.

  5. Functional Task Test: 3. Skeletal Muscle Performance Adaptations to Space Flight

    Science.gov (United States)

    Ryder, Jeffrey W.; Wickwire, P. J.; Buxton, R. E.; Bloomberg, J. J.; Ploutz-Snyder, L.

    2011-01-01

    The functional task test is a multi-disciplinary study investigating how space-flight induced changes to physiological systems impacts functional task performance. Impairment of neuromuscular function would be expected to negatively affect functional performance of crewmembers following exposure to microgravity. This presentation reports the results for muscle performance testing in crewmembers. Functional task performance will be presented in the abstract "Functional Task Test 1: sensory motor adaptations associated with postflight alternations in astronaut functional task performance." METHODS: Muscle performance measures were obtained in crewmembers before and after short-duration space flight aboard the Space Shuttle and long-duration International Space Station (ISS) missions. The battery of muscle performance tests included leg press and bench press measures of isometric force, isotonic power and total work. Knee extension was used for the measurement of central activation and maximal isometric force. Upper and lower body force steadiness control were measured on the bench press and knee extension machine, respectively. Tests were implemented 60 and 30 days before launch, on landing day (Shuttle crew only), and 6, 10 and 30 days after landing. Seven Space Shuttle crew and four ISS crew have completed the muscle performance testing to date. RESULTS: Preliminary results for Space Shuttle crew reveal significant reductions in the leg press performance metrics of maximal isometric force, power and total work on R+0 (pperformance metrics were observed in returning Shuttle crew and these adaptations are likely contributors to impaired functional tasks that are ambulatory in nature (See abstract Functional Task Test: 1). Interestingly, no significant changes in central activation capacity were detected. Therefore, impairments in muscle function in response to short-duration space flight are likely myocellular rather than neuromotor in nature.

  6. Performance of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Aspergillus, Scedosporium, and Fusarium spp. in the Australian Clinical Setting

    Science.gov (United States)

    Sleiman, Sue; Halliday, Catriona L.; Chapman, Belinda; Brown, Mitchell; Nitschke, Joanne; Lau, Anna F.

    2016-01-01

    We developed an Australian database for the identification of Aspergillus, Scedosporium, and Fusarium species (n = 28) by matrix-assisted laser desorption ionization−time of flight mass spectrometry (MALDI-TOF MS). In a challenge against 117 isolates, species identification significantly improved when the in-house-built database was combined with the Bruker Filamentous Fungi Library compared with that for the Bruker library alone (Aspergillus, 93% versus 69%; Fusarium, 84% versus 42%; and Scedosporium, 94% versus 18%, respectively). PMID:27252460

  7. Electronic aids to the identification of bats in flight and to their study under natural conditions

    NARCIS (Netherlands)

    Watson, Andrew

    1970-01-01

    Positive identification of bats usually requires visual inspection at close range or for a bat to be held in the hand. There are many people, particularly conservationists, who would like to study bats in their natural environment without disturbing them. The task of preparing distribution maps is

  8. Design, construction, test and field support of a containerless payload package for rocket flight. [electromagnetic heating and confinement

    Science.gov (United States)

    1977-01-01

    The performance of a device for electromagnetically heating and positioning containerless melts during space processing was evaluated during a 360 second 0-g suborbital sounding rocket flight. Components of the electromagnetic containerless processing package (ECPP), its operation, and interface with the rocket are described along with flight and qualification tests results.

  9. Biometric identification devices -- Laboratory testing vs. real life

    International Nuclear Information System (INIS)

    Ahrens, J.S.

    1997-01-01

    For over fifteen years Sandia National Laboratories has been involved in laboratory testing of biometric identification devices. The key concept of biometric identification devices is the ability for the system to identify some unique aspect of the individual rather than some object a person may be carrying or some password they are required to know. Tests were conducted to verify manufacturer's performance claims, to determine strengths/weaknesses of devices, and to determine devices that meet the US Department of energy's needs. However, during recent field installation, significantly different performance was observed than was predicted by laboratory tests. Although most people using the device believed it operated adequately, the performance observed was over an order of magnitude worse than predicted. The search for reasons behind this gap between the predicted and the actual performance has revealed many possible contributing factors. As engineers, the most valuable lesson to be learned from this experience is the value of scientists and engineers with (1) common sense, (2) knowledge of human behavior, (3) the ability to observe the real world, and (4) the capability to realize the significant differences between controlled experiments and actual installations

  10. Stability and control of the Gossamer human powered aircraft by analysis and flight test

    Science.gov (United States)

    Jex, H. R.; Mitchell, D. G.

    1982-01-01

    The slow flight speed, very light wing loading, and neutral stability of the Gossamer Condor and the Gossamer Albatross emphasized apparent-mass aerodynamic effects and unusual modes of motion response. These are analyzed, approximated, and discussed, and the resulting transfer functions and dynamic properties are summarized and compared. To verify these analytical models, flight tests were conducted with and electrically powered Gossamer Albatross II. Sensors were installed and their outputs were telemetered to records on the ground. Frequency sweeps of the various controls were made and the data were reduced to frequency domain measures. Results are given for the response of: pitch rate, airspeed and normal acceleration from canard-elevator deflection; roll rate and yaw rate from canard-rudder tilt; and roll rate and yaw rate from wing warp. The reliable data are compared with the analytical predictions.

  11. STS-114: Discovery TCDT Flight Crew Test Media Event at Pad 39-B

    Science.gov (United States)

    2005-01-01

    The STS-114 Space Shuttle Discovery Terminal Countdown Demonstration Test (TCDT) flight crew is shown at Pad 39-B. Eileen Collins, Commander introduces the astronauts. Andrew Thomas, mission specialist talks about his primary responsibility of performing boom inspections, Wendy Lawrence, Mission Specialist 4 (MS4) describes her role as the robotic arm operator supporting Extravehicular Activities (EVA), Stephen Robinson, Mission Specialist 3 (MS3) talks about his role as flight engineer, Charlie Camarda, Mission Specialist 5 (MS5) says that his duties are to perform boom operations, transfer operations from the space shuttle to the International Space Station and spacecraft rendezvous. Soichi Noguchi, Mission Specialist 1 (MS1) from JAXA, introduces himself as Extravehicular Activity 1 (EVA1), and Jim Kelley, Pilot will operate the robotic arm and perform pilot duties. Questions from the news media about the safety of the external tank, going to the International Space Station and returning, EVA training, and thoughts about the Space Shuttle Columbia crew are answered.

  12. VUV testing of science cameras at MSFC: QE measurement of the CLASP flight cameras

    Science.gov (United States)

    Champey, P.; Kobayashi, K.; Winebarger, A.; Cirtain, J.; Hyde, D.; Robertson, B.; Beabout, B.; Beabout, D.; Stewart, M.

    2015-08-01

    The NASA Marshall Space Flight Center (MSFC) has developed a science camera suitable for sub-orbital missions for observations in the UV, EUV and soft X-ray. Six cameras were built and tested for the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP), a joint MSFC, National Astronomical Observatory of Japan (NAOJ), Instituto de Astrofisica de Canarias (IAC) and Institut D'Astrophysique Spatiale (IAS) sounding rocket mission. The CLASP camera design includes a frame-transfer e2v CCD57-10 512 × 512 detector, dual channel analog readout and an internally mounted cold block. At the flight CCD temperature of -20C, the CLASP cameras exceeded the low-noise performance requirements (UV, EUV and X-ray science cameras at MSFC.

  13. Orion Exploration Flight Test-l (EFT -1) Absolute Navigation Design

    Science.gov (United States)

    Sud, Jastesh; Gay, Robert; Holt, Greg; Zanetti, Renato

    2014-01-01

    Scheduled to launch in September 2014 atop a Delta IV Heavy from the Kennedy Space Center, the Orion Multi-Purpose-Crew-Vehicle (MPCV's) maiden flight dubbed "Exploration Flight Test -1" (EFT-1) intends to stress the system by placing the uncrewed vehicle on a high-energy parabolic trajectory replicating conditions similar to those that would be experienced when returning from an asteroid or a lunar mission. Unique challenges associated with designing the navigation system for EFT-1 are presented in the narrative with an emphasis on how redundancy and robustness influenced the architecture. Two Inertial Measurement Units (IMUs), one GPS receiver and three barometric altimeters (BALTs) comprise the navigation sensor suite. The sensor data is multiplexed using conventional integration techniques and the state estimate is refined by the GPS pseudorange and deltarange measurements in an Extended Kalman Filter (EKF) that employs the UDUT decomposition approach. The design is substantiated by simulation results to show the expected performance.

  14. Space Environmental Effects Testing Capability at the Marshall Space Flight Center

    Science.gov (United States)

    DeWittBurns, H.; Craven, Paul; Finckenor, Miria; Nehls, Mary; Schneider, Todd; Vaughn, Jason

    2012-01-01

    Understanding the effects of the space environment on materials and systems is fundamental and essential for mission success. If not properly understood and designed for, the effects of the environment can lead to degradation of materials, reduction of functional lifetime, and system failure. In response to this need, the Marshall Space Flight Center has developed world class Space Environmental Effects (SEE) expertise and test facilities to simulate the space environment. Capabilities include multiple unique test systems comprising the most complete SEE testing capability available. These test capabilities include charged particle radiation (electrons, protons, ions), ultraviolet radiation (UV), vacuum ultraviolet radiation (VUV), atomic oxygen, plasma effects, space craft charging, lunar surface and planetary effects, vacuum effects, and hypervelocity impacts as well as the combination of these capabilities. In addition to the uniqueness of the individual test capabilities, MSFC is the only NASA facility where the effects of the different space environments can be tested in one location. Combined with additional analytical capabilities for pre- and post-test evaluation, MSFC is a one-stop shop for materials testing and analysis. The SEE testing and analysis are performed by a team of award winning experts nationally recognized for their contributions in the study of the effects of the space environment on materials and systems. With this broad expertise in space environmental effects and the variety of test systems and equipment available, MSFC is able to customize tests with a demonstrated ability to rapidly adapt and reconfigure systems to meet customers needs. Extensive flight experiment experience bolsters this simulation and analysis capability with a comprehensive understanding of space environmental effects.

  15. System identification of a small low-cost unmanned aerial vehicle using flight data from low-cost sensors

    Science.gov (United States)

    Hoffer, Nathan Von

    Remote sensing has traditionally been done with satellites and manned aircraft. While. these methods can yield useful scientificc data, satellites and manned aircraft have limitations in data frequency, process time, and real time re-tasking. Small low-cost unmanned aerial vehicles (UAVs) provide greater possibilities for personal scientic research than traditional remote sensing platforms. Precision aerial data requires an accurate vehicle dynamics model for controller development, robust flight characteristics, and fault tolerance. One method of developing a model is system identification (system ID). In this thesis system ID of a small low-cost fixed-wing T-tail UAV is conducted. The linerized longitudinal equations of motion are derived from first principles. Foundations of Recursive Least Squares (RLS) are presented along with RLS with an Error Filtering Online Learning scheme (EFOL). Sensors, data collection, data consistency checking, and data processing are described. Batch least squares (BLS) and BLS with EFOL are used to identify aerodynamic coecoefficients of the UAV. Results of these two methods with flight data are discussed.

  16. Flight Testing of Night Vision Systems in Rotorcraft (Test en vol de systemes de vision nocturne a bord des aeronefs a voilure tournante)

    Science.gov (United States)

    2007-07-01

    Test Engineer GVE Good Visual Environment HMD Head Mounted Displays HQR Handling Quality Rating HUD Heads Up Display IFR Instrument Flight...may take the form of general questionnaires such as the China Lake Situational Awareness Rating Scale, the Situational Awareness Global Assessment...performed in 5-ft decrements. IFR transit flight duties should also be performed, when simulating flight in IMC. In all cases, internal lighting must

  17. Matrix-assisted laser desorption ionization time of flight mass spectrometry and diagnostic testing for prosthetic joint infection in the clinical microbiology laboratory.

    Science.gov (United States)

    Peel, Trisha N; Cole, Nicolynn C; Dylla, Brenda L; Patel, Robin

    2015-03-01

    Identification of pathogen(s) associated with prosthetic joint infection (PJI) is critical for patient management. Historically, many laboratories have not routinely identified organisms such as coagulase-negative staphylococci to the species level. The advent of matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has enhanced clinical laboratory capacity for accurate species-level identification. The aim of this study was to describe the species-level identification of microorganisms isolated from periprosthetic tissue and fluid specimens using MALDI-TOF MS alongside other rapid identification tests in a clinical microbiology laboratory. Results of rapid identification of bacteria isolated from periprosthetic joint fluid and/or tissue specimens were correlated with clinical findings at Mayo Clinic, Rochester, Minnesota, between May 2012 and May 2013. There were 178 PJI and 82 aseptic failure (AF) cases analyzed, yielding 770 organisms (median, 3/subject; range, 1-19/subject). MALDI-TOF MS was employed for the identification of 455 organisms (59%) in 197 subjects (123 PJIs and 74 AFs), with 89% identified to the species level using this technique. Gram-positive bacteria accounted for 68% and 93% of isolates in PJI and AF, respectively. However, the profile of species associated with infection compared to specimen contamination differed. Staphylococcus aureus and Staphylococcus caprae were always associated with infection, Staphylococcus epidermidis and Staphylococcus lugdunensis were equally likely to be a pathogen or a contaminant, whereas the other coagulase-negative staphylococci were more frequently contaminants. Most streptococcal and Corynebacterium isolates were pathogens. The likelihood that an organism was a pathogen or contaminant differed with the prosthetic joint location, particularly in the case of Propionibacterium acnes. MALDI-TOF MS is a valuable tool for the identification of bacteria isolated from patients

  18. New Cryogenic Optical Test Capability at Marshall Space Flight Center's Space Optics Manufacturing Technology Center

    Science.gov (United States)

    Kegley, Jeff; Burdine, Robert V. (Technical Monitor)

    2002-01-01

    A new cryogenic optical testing capability exists at Marshall Space Flight Center's Space Optics Manufacturing Technology Center (SOMTC). SOMTC has been performing optical wavefront testing at cryogenic temperatures since 1999 in the X-ray Cryogenic Test Facility's (XRCF's) large vacuum chamber. Recently the cryogenic optical testing capability has been extended to a smaller vacuum chamber. This smaller horizontal cylindrical vacuum chamber has been outfitted with a helium-cooled liner that can be connected to the facility's helium refrigeration system bringing the existing kilowatt of refrigeration capacity to bear on a 1 meter diameter x 2 meter long test envelope. Cryogenic environments to less than 20 Kelvin are now possible in only a few hours. SOMTC's existing instruments (the Instantaneous Phase-shifting Interferometer (IPI) from ADE Phase-Shift Technologies and the PhaseCam from 4D Vision Technologies) view the optic under test through a 150 mm clear aperture BK-7 window. Since activation and chamber characterization tests in September 2001, the new chamber has been used to perform a cryogenic (less than 30 Kelvin) optical test of a 22.5 cm diameter x 127 cm radius of curvature Si02 mirror, a cryogenic survival (less than 30 Kelvin) test of an adhesive, and a cryogenic cycle (less than 20 Kelvin) test of a ULE mirror. A vibration survey has also been performed on the test chamber. Chamber specifications and performance data, vibration environment data, and limited test results will be presented.

  19. Case Study: Test Results of a Tool and Method for In-Flight, Adaptive Control System Verification on a NASA F-15 Flight Research Aircraft

    Science.gov (United States)

    Jacklin, Stephen A.; Schumann, Johann; Guenther, Kurt; Bosworth, John

    2006-01-01

    Adaptive control technologies that incorporate learning algorithms have been proposed to enable autonomous flight control and to maintain vehicle performance in the face of unknown, changing, or poorly defined operating environments [1-2]. At the present time, however, it is unknown how adaptive algorithms can be routinely verified, validated, and certified for use in safety-critical applications. Rigorous methods for adaptive software verification end validation must be developed to ensure that. the control software functions as required and is highly safe and reliable. A large gap appears to exist between the point at which control system designers feel the verification process is complete, and when FAA certification officials agree it is complete. Certification of adaptive flight control software verification is complicated by the use of learning algorithms (e.g., neural networks) and degrees of system non-determinism. Of course, analytical efforts must be made in the verification process to place guarantees on learning algorithm stability, rate of convergence, and convergence accuracy. However, to satisfy FAA certification requirements, it must be demonstrated that the adaptive flight control system is also able to fail and still allow the aircraft to be flown safely or to land, while at the same time providing a means of crew notification of the (impending) failure. It was for this purpose that the NASA Ames Confidence Tool was developed [3]. This paper presents the Confidence Tool as a means of providing in-flight software assurance monitoring of an adaptive flight control system. The paper will present the data obtained from flight testing the tool on a specially modified F-15 aircraft designed to simulate loss of flight control faces.

  20. Comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry in the identification of organic compounds in atmospheric aerosols from coniferous forest

    NARCIS (Netherlands)

    Kallio, M.; Jussila, M.; Rissanen, T.; Anttila, P.; Hartonen, K.; Reissell, A.; Vreuls, R.J.J.; Adahchour, M.; Hyotylainen, T.

    2006-01-01

    Comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC × GC-TOF-MS) was applied in the identification of organic compounds in atmospheric aerosols from coniferous forest. The samples were collected at Hyytiälä, Finland, as part of the QUEST campaign, in

  1. Identification of Wheat Varieties Using Matrix-assisted Laser Desorption/Ionisation Time-of-flight Mass Spectrometry and an Artificial Neural network

    DEFF Research Database (Denmark)

    Bloch, Helle Aagaard; Kesmir, Can; Petersen, Marianne Kjerstine

    1999-01-01

    A novel tool for variety identification of wheat (Triticum aestivum L,) has been developed: an artificial neural network (ANN) is used to classify the gliadin fraction analysed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS). The robustness...

  2. VUV Testing of Science Cameras at MSFC: QE Measurement of the CLASP Flight Cameras

    Science.gov (United States)

    Champey, Patrick R.; Kobayashi, Ken; Winebarger, A.; Cirtain, J.; Hyde, D.; Robertson, B.; Beabout, B.; Beabout, D.; Stewart, M.

    2015-01-01

    The NASA Marshall Space Flight Center (MSFC) has developed a science camera suitable for sub-orbital missions for observations in the UV, EUV and soft X-ray. Six cameras were built and tested for the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP), a joint National Astronomical Observatory of Japan (NAOJ) and MSFC sounding rocket mission. The CLASP camera design includes a frame-transfer e2v CCD57-10 512x512 detector, dual channel analog readout electronics and an internally mounted cold block. At the flight operating temperature of -20 C, the CLASP cameras achieved the low-noise performance requirements (less than or equal to 25 e- read noise and greater than or equal to 10 e-/sec/pix dark current), in addition to maintaining a stable gain of approximately equal to 2.0 e-/DN. The e2v CCD57-10 detectors were coated with Lumogen-E to improve quantum efficiency (QE) at the Lyman- wavelength. A vacuum ultra-violet (VUV) monochromator and a NIST calibrated photodiode were employed to measure the QE of each camera. Four flight-like cameras were tested in a high-vacuum chamber, which was configured to operate several tests intended to verify the QE, gain, read noise, dark current and residual non-linearity of the CCD. We present and discuss the QE measurements performed on the CLASP cameras. We also discuss the high-vacuum system outfitted for testing of UV and EUV science cameras at MSFC.

  3. Description and Flight Test Results of the NASA F-8 Digital Fly-by-Wire Control System

    Science.gov (United States)

    1975-01-01

    A NASA program to develop digital fly-by-wire (DFBW) technology for aircraft applications is discussed. Phase I of the program demonstrated the feasibility of using a digital fly-by-wire system for aircraft control through developing and flight testing a single channel system, which used Apollo hardware, in an F-8C airplane. The objective of Phase II of the program is to establish a technology base for designing practical DFBW systems. It will involve developing and flight testing a triplex digital fly-by-wire system using state-of-the-art airborne computers, system hardware, software, and redundancy concepts. The papers included in this report describe the Phase I system and its development and present results from the flight program. Man-rated flight software and the effects of lightning on digital flight control systems are also discussed.

  4. 14 CFR 61.405 - What tests do I have to take to obtain a flight instructor certificate with a sport pilot rating?

    Science.gov (United States)

    2010-01-01

    ... flight instructor certificate with a sport pilot rating? 61.405 Section 61.405 Aeronautics and Space..., FLIGHT INSTRUCTORS, AND GROUND INSTRUCTORS Flight Instructors With a Sport Pilot Rating § 61.405 What tests do I have to take to obtain a flight instructor certificate with a sport pilot rating? To obtain a...

  5. Optimization of the design of X-Calibur for a long-duration balloon flight and results from a one-day test flight

    Science.gov (United States)

    Kislat, Fabian; Abarr, Quin; Beheshtipour, Banafsheh; De Geronimo, Gianluigi; Dowkontt, Paul; Tang, Jason; Krawczynski, Henric

    2018-01-01

    X-ray polarimetry promises exciting insights into the physics of compact astrophysical objects by providing two observables: the polarization fraction and angle as function of energy. X-Calibur is a balloon-borne hard x-ray scattering polarimeter for the 15- to 60-keV energy range. After the successful test flight in September 2016, the instrument is now being prepared for a long-duration balloon (LDB) flight in December 2018 through January 2019. During the LDB flight, X-Calibur will make detailed measurements of the polarization of Vela X-1 and constrain the polarization of a sample of between 4 and 9 additional sources. We describe the upgraded polarimeter design, including the use of a beryllium scattering element, lower-noise front-end electronics, and an improved fully active CsI(Na) anticoincidence shield, which will significantly increase the instrument sensitivity. We present estimates of the improved polarimeter performance based on simulations and laboratory measurements. We present some of the results from the 2016 flight and show that we solved several problems, which led to a reduced sensitivity during the 2016 flight. We end with a description of the planned Vela X-1 observations, including a Swift/BAT-guided observation strategy.

  6. Testing an emerging paradigm in migration ecology shows surprising differences in efficiency between flight modes.

    Directory of Open Access Journals (Sweden)

    Adam E Duerr

    Full Text Available To maximize fitness, flying animals should maximize flight speed while minimizing energetic expenditure. Soaring speeds of large-bodied birds are determined by flight routes and tradeoffs between minimizing time and energetic costs. Large raptors migrating in eastern North America predominantly glide between thermals that provide lift or soar along slopes or ridgelines using orographic lift (slope soaring. It is usually assumed that slope soaring is faster than thermal gliding because forward progress is constant compared to interrupted progress when birds pause to regain altitude in thermals. We tested this slope-soaring hypothesis using high-frequency GPS-GSM telemetry devices to track golden eagles during northbound migration. In contrast to expectations, flight speed was slower when slope soaring and eagles also were diverted from their migratory path, incurring possible energetic costs and reducing speed of progress towards a migratory endpoint. When gliding between thermals, eagles stayed on track and fast gliding speeds compensated for lack of progress during thermal soaring. When thermals were not available, eagles minimized migration time, not energy, by choosing energetically expensive slope soaring instead of waiting for thermals to develop. Sites suited to slope soaring include ridges preferred for wind-energy generation, thus avian risk of collision with wind turbines is associated with evolutionary trade-offs required to maximize fitness of time-minimizing migratory raptors.

  7. Testing an emerging paradigm in migration ecology shows surprising differences in efficiency between flight modes.

    Science.gov (United States)

    Duerr, Adam E; Miller, Tricia A; Lanzone, Michael; Brandes, Dave; Cooper, Jeff; O'Malley, Kieran; Maisonneuve, Charles; Tremblay, Junior; Katzner, Todd

    2012-01-01

    To maximize fitness, flying animals should maximize flight speed while minimizing energetic expenditure. Soaring speeds of large-bodied birds are determined by flight routes and tradeoffs between minimizing time and energetic costs. Large raptors migrating in eastern North America predominantly glide between thermals that provide lift or soar along slopes or ridgelines using orographic lift (slope soaring). It is usually assumed that slope soaring is faster than thermal gliding because forward progress is constant compared to interrupted progress when birds pause to regain altitude in thermals. We tested this slope-soaring hypothesis using high-frequency GPS-GSM telemetry devices to track golden eagles during northbound migration. In contrast to expectations, flight speed was slower when slope soaring and eagles also were diverted from their migratory path, incurring possible energetic costs and reducing speed of progress towards a migratory endpoint. When gliding between thermals, eagles stayed on track and fast gliding speeds compensated for lack of progress during thermal soaring. When thermals were not available, eagles minimized migration time, not energy, by choosing energetically expensive slope soaring instead of waiting for thermals to develop. Sites suited to slope soaring include ridges preferred for wind-energy generation, thus avian risk of collision with wind turbines is associated with evolutionary trade-offs required to maximize fitness of time-minimizing migratory raptors.

  8. Flight test of a head-worn display as an equivalent-HUD for terminal operations

    Science.gov (United States)

    Shelton, K. J.; Arthur, J. J.; Prinzel, L. J.; Nicholas, S. N.; Williams, S. P.; Bailey, R. E.

    2015-05-01

    Research, development, test, and evaluation of flight deck interface technologies is being conducted by NASA to proactively identify, develop, and mature tools, methods, and technologies for improving overall aircraft safety of new and legacy vehicles operating in the Next Generation Air Transportation System (NextGen). Under NASA's Aviation Safety Program, one specific area of research is the use of small Head-Worn Displays (HWDs) as a potential equivalent display to a Head-up Display (HUD). Title 14 of the US CFR 91.175 describes a possible operational credit which can be obtained with airplane equipage of a HUD or an "equivalent"' display combined with Enhanced Vision (EV). A successful HWD implementation may provide the same safety and operational benefits as current HUD-equipped aircraft but for significantly more aircraft in which HUD installation is neither practical nor possible. A flight test was conducted to evaluate if the HWD, coupled with a head-tracker, can provide an equivalent display to a HUD. Approach and taxi testing was performed on-board NASA's experimental King Air aircraft in various visual conditions. Preliminary quantitative results indicate the HWD tested provided equivalent HUD performance, however operational issues were uncovered. The HWD showed significant potential as all of the pilots liked the increased situation awareness attributable to the HWD's unique capability of unlimited field-of-regard.

  9. Flying Boresight for Advanced Testing and Calibration of Tracking Antennas and Flight Path Simulations

    Science.gov (United States)

    Hafner, D.

    2015-09-01

    The application of ground-based boresight sources for calibration and testing of tracking antennas usually entails various difficulties, mostly due to unwanted ground effects. To avoid this problem, DLR MORABA developed a small, lightweight, frequency-adjustable S-band boresight source, mounted on a small remote-controlled multirotor aircraft. Highly accurate GPS-supported, position and altitude control functions allow both, very steady positioning of the aircraft in mid-air, and precise waypoint-based, semi-autonomous flights. In contrast to fixed near-ground boresight sources this flying setup enables to avoid obstructions in the Fresnel zone between source and antenna. Further, it minimizes ground reflections and other multipath effects which can affect antenna calibration. In addition, the large operating range of a flying boresight simplifies measurements in the far field of the antenna and permits undisturbed antenna pattern tests. A unique application is the realistic simulation of sophisticated flight paths, including overhead tracking and demanding trajectories of fast objects such as sounding rockets. Likewise, dynamic tracking tests are feasible which provide crucial information about the antenna pedestal performance — particularly at high elevations — and reveal weaknesses in the autotrack control loop of tracking antenna systems. During acceptance tests of MORABA's new tracking antennas, a manned aircraft was never used, since the Flying Boresight surpassed all expectations regarding usability, efficiency, and precision. Hence, it became an integral part of MORABA's standard antenna setup and calibration procedures.

  10. Celebrity Influence and Identification: A Test of the Angelina Effect.

    Science.gov (United States)

    Kosenko, Kami A; Binder, Andrew R; Hurley, Ryan

    2016-01-01

    Angelina Jolie's announcement that she is a BRCA1 carrier and has had a prophylactic bilateral mastectomy was met with widespread support as well as speculation about its possible impact on the public. These speculations were the subject of a Time magazine cover story titled "The Angelina Effect" (Kluger et al., 2013 ). Although there is anecdotal evidence to support this hypothesized Angelina effect, empirical tests are lacking. To explore possible links between Angelina's announcement and public health, we surveyed 356 adults immediately after the announcement. Guided by a model of celebrity influence, the survey assessed participants' demographics and health history, identification and parasocial interaction with Jolie, and genetic testing intentions. Results supported the model's predictions and provided preliminary evidence of an Angelina effect.

  11. Testing the time-of-flight model for flagellar length sensing.

    Science.gov (United States)

    Ishikawa, Hiroaki; Marshall, Wallace F

    2017-11-07

    Cilia and flagella are microtubule-based organelles that protrude from the surface of most cells, are important to the sensing of extracellular signals, and make a driving force for fluid flow. Maintenance of flagellar length requires an active transport process known as intraflagellar transport (IFT). Recent studies reveal that the amount of IFT injection negatively correlates with the length of flagella. These observations suggest that a length-dependent feedback regulates IFT. However, it is unknown how cells recognize the length of flagella and control IFT. Several theoretical models try to explain this feedback system. We focused on one of the models, the "time-of-flight" model, which measures the length of flagella on the basis of the travel time of IFT protein in the flagellar compartment. We tested the time-of-flight model using Chlamydomonas dynein mutant cells, which show slower retrograde transport speed. The amount of IFT injection in dynein mutant cells was higher than that in control cells. This observation does not support the prediction of the time-of-flight model and suggests that Chlamydomonas uses another length-control feedback system rather than that described by the time-of-flight model. © 2017 Ishikawa and Marshall. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  12. Species Identification and Delineation of Pathogenic Mucorales by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.

    Science.gov (United States)

    Shao, Jin; Wan, Zhe; Li, Ruoyu; Yu, Jin

    2018-04-01

    This study aimed to validate the effectiveness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification of filamentous fungi of the order Mucorales. A total of 111 isolates covering six genera preserved at the Research Center for Medical Mycology of Peking University were selected for MALDI-TOF MS analysis. We emphasized the study of 23 strains of Mucor irregularis predominantly isolated from patients in China. We first used the Bruker Filamentous Fungi library (v1.0) to identify all 111 isolates. To increase the identification rate, we created a compensatory in-house database, the Beijing Medical University (BMU) database, using 13 reference strains covering 6 species, including M. irregularis , Mucor hiemalis , Mucor racemosus , Cunninghamella bertholletiae , Cunninghamella phaeospora , and Cunninghamella echinulata All 111 isolates were then identified by MALDI-TOF MS using a combination of the Bruker library and BMU database. MALDI-TOF MS identified 55 (49.5%) and 74 (66.7%) isolates at the species and genus levels, respectively, using the Bruker Filamentous Fungi library v1.0 alone. A combination of the Bruker library and BMU database allowed MALDI-TOF MS to identify 90 (81.1%) and 111 (100%) isolates at the species and genus levels, respectively, with a significantly increased accuracy rate. MALDI-TOF MS poorly identified Mucorales when the Bruker library was used alone due to its lack of some fungal species. In contrast, this technique perfectly identified M. irregularis after main spectrum profiles (MSPs) of relevant reference strains were added to the Bruker library. With an expanded Bruker library, MALDI-TOF MS is an effective tool for the identification of pathogenic Mucorales. Copyright © 2018 American Society for Microbiology.

  13. Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    De Carolis, E; Posteraro, B; Lass-Flörl, C; Vella, A; Florio, A R; Torelli, R; Girmenia, C; Colozza, C; Tortorano, A M; Sanguinetti, M; Fadda, G

    2012-05-01

    Accurate species discrimination of filamentous fungi is essential, because some species have specific antifungal susceptibility patterns, and misidentification may result in inappropriate therapy. We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for species identification through direct surface analysis of the fungal culture. By use of culture collection strains representing 55 species of Aspergillus, Fusarium and Mucorales, a reference database was established for MALDI-TOF MS-based species identification according to the manufacturer's recommendations for microflex measurements and MALDI BioTyper 2.0 software. The profiles of young and mature colonies were analysed for each of the reference strains, and species-specific spectral fingerprints were obtained. To evaluate the database, 103 blind-coded fungal isolates collected in the routine clinical microbiology laboratory were tested. As a reference method for species designation, multilocus sequencing was used. Eighty-five isolates were unequivocally identified to the species level (≥99% sequence similarity); 18 isolates producing ambiguous results at this threshold were initially rated as identified to the genus level only. Further molecular analysis definitively assigned these isolates to the species Aspergillus oryzae (17 isolates) and Aspergillus flavus (one isolate), concordant with the MALDI-TOF MS results. Excluding nine isolates that belong to the fungal species not included in our reference database, 91 (96.8%) of 94 isolates were identified by MALDI-TOF MS to the species level, in agreement with the results of the reference method; three isolates were identified to the genus level. In conclusion, MALDI-TOF MS is suitable for the routine identification of filamentous fungi in a medical microbiology laboratory. © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.

  14. 76 FR 64229 - Function and Reliability Flight Testing for Turbine-Powered Airplanes Weighing 6,000 Pounds or Less

    Science.gov (United States)

    2011-10-18

    ... F & R flight testing regardless of the airplane's systems complexity or level of automation. After... than some transport category airplanes of the 1960s and earlier. The NPRM contains more of the...

  15. 75 FR 18134 - Function and Reliability Flight Testing for Turbine-Powered Airplanes Weighing 6,000 Pounds or Less

    Science.gov (United States)

    2010-04-09

    ... undergo F & R flight testing regardless of the airplane's systems complexity or level of automation. After... airplanes that weigh 6,000 pounds or less to be more complex and integrated than some transport category...

  16. Guidance, Navigation and Control (GN and C) Design Overview and Flight Test Results from NASA's Max Launch Abort System (MLAS)

    Science.gov (United States)

    Dennehy, Cornelius J.; Lanzi, Raymond J.; Ward, Philip R.

    2010-01-01

    The National Aeronautics and Space Administration Engineering and Safety Center designed, developed and flew the alternative Max Launch Abort System (MLAS) as risk mitigation for the baseline Orion spacecraft launch abort system already in development. The NESC was tasked with both formulating a conceptual objective system design of this alternative MLAS as well as demonstrating this concept with a simulated pad abort flight test. Less than 2 years after Project start the MLAS simulated pad abort flight test was successfully conducted from Wallops Island on July 8, 2009. The entire flight test duration was 88 seconds during which time multiple staging events were performed and nine separate critically timed parachute deployments occurred as scheduled. This paper provides an overview of the guidance navigation and control technical approaches employed on this rapid prototyping activity; describes the methodology used to design the MLAS flight test vehicle; and lessons that were learned during this rapid prototyping project are also summarized.

  17. Pilot Field Test: Use of a Compression Garment During a Stand Test After Long-Duration Space Flight

    Science.gov (United States)

    Laurie, S. S.; Stenger, M. B.; Phillips, T. R.; Lee, S. M. C.; Cerisano, J.; Kofman, I.; Reschke, M.

    2016-01-01

    Orthostatic intolerance (OI) is a concern for astronauts returning from long-duration space flight. One countermeasure that has been used to protect against OI after short-duration bed rest and space flight is the use of lower body and abdominal compression garments. However, since the end of the Space Shuttle era we have not been able to test crewmembers during the first 24 hours after landing on Earth. NASA's Pilot Field Test provided us the opportunity to test cardiovascular responses of crewmembers wearing the Russian Kentavr compression garment during a stand test at multiple time points throughout the first 24 hours after landing. HYPOTHESIS We hypothesized that the Kentavr compression garment would prevent an increase in heart rate (HR) >15 bpm during a 3.5-min stand test. METHODS: The Pilot Field Test was conducted up to 3 times during the first 24 hours after crewmembers returned to Earth: (1) either in a tent adjacent to the Soyuz landing site in Kazakhstan (approx.1 hr) or after transportation to the Karaganda airport (approx. 4 hr); (2) during a refueling stop in Scotland (approx.12 hr); and (3) upon return to NASA Johnson Space Center (JSC) (approx.24 hr). We measured HR and arterial pressure (finger photoplethysmography) for 2 min while the crewmember was prone and throughout 3.5 min of quiet standing. Eleven crewmembers consented to participate; however, 2 felt too ill to start the test and 1 stopped 30 sec into the stand portion of the test. Of the remaining 8 crewmembers, 2 did not wear the Russian Kentavr compression garment. Because of inclement weather at the landing site, 5 crewmembers were flown by helicopter to the Karaganda airport before initial testing and received intravenous saline before completing the stand test. One of these crewmembers wore only the portion of the Russian Kentavr compression garment that covered the lower leg and thus lacked thigh and abdominal compression. All crewmembers continued wearing the Russian Kentavr

  18. Flight test of a spin parachute for use with a Super Arcas sounding rocket

    Science.gov (United States)

    Silbert, M. N.

    1975-01-01

    The development and flight testing of a specially configured 16.6 ft Disc Band Gap (DBG) Spin Parachute is discussed. The parachute is integrated with a modified Super Arcas launch vehicle. Total payload weight was 17.6 lbs including the Spin Parachute and a scientific payload, and lift-off weight was 100.3 lbs. The Super Arcas vehicle was despun from 18.4 cps. After payload separation at 244,170 ft the Spin Parachute and its payload attained a maximum spin rate of 2.4 cps. Total suspended weight of the Spin Parachute and its payload was 14.64 lbs.

  19. A measurement of Omega from the North American test flight of Boomerang

    International Nuclear Information System (INIS)

    Melchiorri, A.; Ade, P.A.R.; De Bernardis, P.; Bock, J.J.; Borrill, J.; Boscaleri, A.; Crill, B.P.; De Troia, G.; Farese, P.; Ferreira, P.G.; Ganga, K.; Gasperis, G. de; Giacometti, M.; Hristov, V.V.; Jaffe, A.H.; Lange, A.E.; Masi, S.; Mauskopf, P.D.; Miglio, L.; Netterfield, C.B.; Pascale, E.; Piacentini, F.; Romeo, G.; Ruhl, J.E.; Vittorio, N.

    1999-01-01

    We use the power spectrum of the Cosmic Microwave Background, measured during the North American test flight of the BOOMERANG experiment, to constrain the geometry of the universe. Within the class of Cold Dark Matter models, we find the overall fractional energy density of the universe, Omega, is constrained to be 0.85 < or = Omega < or = 1.25 at the 68 percent confidence level. Combined with the COBE measurement and the high redshift supernovae data we obtain new constraints on the fractional matter density and the cosmological constant

  20. A measurement of $\\Omega$ from the North American test flight of BOOMERANG

    CERN Document Server

    Melchiorri, A; De Bernardis, P; Bock, J J; Borrill, J; Boscaleri, A; Crill, B P; De Troia, G; Farese, P; Ferreira, P G; Ganga, K; De Gasperis, G; Giacometti, M; Hristov, V V; Jaffe, A H; Lange, A E; Masi, S; Mauskopf, P D; Miglio, L; Netterfield, C B; Pascale, E; Piacentini, F; Romeo, G

    2000-01-01

    We use the angular power spectrum of the Cosmic Microwave Background, measured during the North American test flight of the BOOMERANG experiment, to constrain the geometry of the universe. Within the class of Cold Dark Matter models, we find that the overall fractional energy density of the universe, Omega, is constrained to be 0.85 < Omega < 1.25 at the 68% confidence level. Combined with the COBE measurement and the high redshift supernovae data we obtain new constraints on the fractional matter density and the cosmological constant.

  1. Application of Computational Fluid Dynamics (CFD) in transonic wind-tunnel/flight-test correlation

    Science.gov (United States)

    Murman, E. M.

    1982-01-01

    The capability for calculating transonic flows for realistic configurations and conditions is discussed. Various phenomena which were modeled are shown to have the same order of magnitude on the influence of predicted results. It is concluded that CFD can make the following contributions to the task of correlating wind tunnel and flight test data: some effects of geometry differences and aeroelastic distortion can be predicted; tunnel wall effects can be assessed and corrected for; and the effects of model support systems and free stream nonuniformities can be modeled.

  2. Testing a new NIF neutron time-of-flight detector with a bibenzyl scintillator on OMEGA.

    Science.gov (United States)

    Glebov, V Yu; Forrest, C; Knauer, J P; Pruyne, A; Romanofsky, M; Sangster, T C; Shoup, M J; Stoeckl, C; Caggiano, J A; Carman, M L; Clancy, T J; Hatarik, R; McNaney, J; Zaitseva, N P

    2012-10-01

    A new neutron time-of-flight (nTOF) detector with a bibenzyl crystal as a scintillator has been designed and manufactured for the National Ignition Facility (NIF). This detector will replace a nTOF20-Spec detector with an oxygenated xylene scintillator currently operational on the NIF to improve the areal-density measurements. In addition to areal density, the bibenzyl detector will measure the D-D and D-T neutron yield and the ion temperature of indirect- and direct-drive-implosion experiments. The design of the bibenzyl detector and results of tests on the OMEGA Laser System are presented.

  3. Test Operations Procedure (TOP) 05-2-543 Enhanced Flight Termination Receiver (EFTR) Range Certification Testing

    Science.gov (United States)

    2011-07-25

    35 5.10 Signal Strength Telemetry Output ( SSTO ) (Test Number 10) ............ 38 5.11 Operational...All command outputs and monitor outputs shall respond properly. TOP 05-2-543 25 July 2011 38 5.10 Signal Strength Telemetry Output ( SSTO ...Test Number 10). a. Purpose. This test verifies that the signal strength telemetry output ( SSTO ) voltage is monotonic and directly related to the

  4. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry: protocol standardization and database expansion for rapid identification of clinically important molds.

    Science.gov (United States)

    Paul, Saikat; Singh, Pankaj; Rudramurthy, Shivaprakash M; Chakrabarti, Arunaloke; Ghosh, Anup K

    2017-12-01

    To standardize the matrix-assisted laser desorption ionization-time of flight mass spectrometry protocols and expansion of existing Bruker Biotyper database for mold identification. Four different sample preparation methods (protocol A, B, C and D) were evaluated. On analyzing each protein extraction method, reliable identification and best log scores were achieved through protocol D. The same protocol was used to identify 153 clinical isolates. Of these 153, 123 (80.3%) were accurately identified by using existing database and remaining 30 (19.7%) were not identified due to unavailability in database. On inclusion of missing main spectrum profile in existing database, all 153 isolates were identified. Matrix-assisted laser desorption ionization-time of flight mass spectrometry can be used for routine identification of clinically important molds.

  5. UAS Integration in the NAS Project: Flight Test 3 Data Analysis of JADEM-Autoresolver Detect and Avoid System

    Science.gov (United States)

    Gong, Chester; Wu, Minghong G.; Santiago, Confesor

    2016-01-01

    The Unmanned Aircraft Systems Integration in the National Airspace System project, or UAS Integration in the NAS, aims to reduce technical barriers related to safety and operational challenges associated with enabling routine UAS access to the NAS. The UAS Integration in the NAS Project conducted a flight test activity, referred to as Flight Test 3 (FT3), involving several Detect-and-Avoid (DAA) research prototype systems between June 15, 2015 and August 12, 2015 at the Armstrong Flight Research Center (AFRC). This report documents the flight testing and analysis results for the NASA Ames-developed JADEM-Autoresolver DAA system, referred to as 'Autoresolver' herein. Four flight test days (June 17, 18, 22, and July 22) were dedicated to Autoresolver testing. The objectives of this test were as follows: 1. Validate CPA prediction accuracy and detect-and-avoid (DAA, formerly known as self-separation) alerting logic in realistic flight conditions. 2. Validate DAA trajectory model including maneuvers. 3. Evaluate TCAS/DAA interoperability. 4. Inform final Minimum Operating Performance Standards (MOPS). Flight test scenarios were designed to collect data to directly address the objectives 1-3. Objective 4, inform final MOPS, was a general objective applicable to the UAS in the NAS project as a whole, of which flight test is a subset. This report presents analysis results completed in support of the UAS in the NAS project FT3 data review conducted on October 20, 2015. Due to time constraints and, to a lesser extent, TCAS data collection issues, objective 3 was not evaluated in this analysis.

  6. Operational Lessons Learned from the Ares I-X Flight Test

    Science.gov (United States)

    Davis, Stephan R.

    2010-01-01

    The Ares I-X flight test, launched in 2009, is the first test of the Ares I crew launch vehicle. This development flight test evaluated the flight dynamics, roll control, and separation events, but also provided early insights into logistical, stacking, launch, and recovery operations for Ares I. Operational lessons will be especially important for NASA as the agency makes the transition from the Space Shuttle to the Constellation Program, which is designed to be less labor-intensive. The mission team itself comprised only 700 individuals over the life of the project compared to the thousands involved in Shuttle and Apollo missions; while missions to and beyond low-Earth orbit obviously will require additional personnel, this lean approach will serve as a model for future Constellation missions. To prepare for Ares I-X, vehicle stacking and launch infrastructure had to be modified at Kennedy Space Center's Vehicle Assembly Building (VAB) as well as Launch Complex (LC) 39B. In the VAB, several platforms and other structures designed for the Shuttle s configuration had to be removed to accommodate the in-line, much taller Ares I-X. Vehicle preparation activities resulted in delays, but also in lessons learned for ground operations personnel, including hardware deliveries, cable routing, transferred work and custodial paperwork. Ares I-X also proved to be a resource challenge, as individuals and ground service equipment (GSE) supporting the mission also were required for Shuttle or Atlas V operations at LC 40/41 at Cape Canaveral Air Force Station. At LC 39B, several Shuttle-specific access arms were removed and others were added to accommodate the in-line Ares vehicle. Ground command, control, and communication (GC3) hardware was incorporated into the Mobile Launcher Platform (MLP). The lightning protection system at LC 39B was replaced by a trio of 600-foot-tall towers connected by a catenary wire to account for the much greater height of the vehicle. Like Shuttle

  7. A flight management algorithm and guidance for fuel-conservative descents in a time-based metered air traffic environment: Development and flight test results

    Science.gov (United States)

    Knox, C. E.

    1984-01-01

    A simple airborne flight management descent algorithm designed to define a flight profile subject to the constraints of using idle thrust, a clean airplane configuration (landing gear up, flaps zero, and speed brakes retracted), and fixed-time end conditions was developed and flight tested in the NASA TSRV B-737 research airplane. The research test flights, conducted in the Denver ARTCC automated time-based metering LFM/PD ATC environment, demonstrated that time guidance and control in the cockpit was acceptable to the pilots and ATC controllers and resulted in arrival of the airplane over the metering fix with standard deviations in airspeed error of 6.5 knots, in altitude error of 23.7 m (77.8 ft), and in arrival time accuracy of 12 sec. These accuracies indicated a good representation of airplane performance and wind modeling. Fuel savings will be obtained on a fleet-wide basis through a reduction of the time error dispersions at the metering fix and on a single-airplane basis by presenting the pilot with guidance for a fuel-efficient descent.

  8. Development of a blood-based molecular biomarker test for identification of schizophrenia before disease onset

    NARCIS (Netherlands)

    M.K. Chan (Man K.); M.-O. Krebs (M-O); D. Cox; P.C. Guest (Paul); R.H. Yolken; H. Rahmoune (Hassan); M. Rothermundt (Matthias); J. Steiner (Johann); F.M. Leweke (Marcus); N.J.M. van Beveren (Nico); D. Niebuhr (David); N. Weber (Natalya); D. Cowan (David); P. Suarez-Pinilla; B. Crespo-Facorro (Benedicto); C. Mam-Lam-Fook; J. Bourgin; R.J. Wenstrup (Richard); R.R. Kaldate; J.D. Cooper (Jason); S. Bahn (Sabine)

    2015-01-01

    markdownabstractRecent research efforts have progressively shifted towards preventative psychiatry and prognostic identification of individuals before disease onset. We describe the development of a serum biomarker test for the identification of individuals at risk of developing schizophrenia based

  9. International Space Station Bacteria Filter Element Post-Flight Testing and Service Life Prediction

    Science.gov (United States)

    Perry, J. L.; von Jouanne, R. G.; Turner, E. H.

    2003-01-01

    The International Space Station uses high efficiency particulate air (HEPA) filters to remove particulate matter from the cabin atmosphere. Known as Bacteria Filter Elements (BFEs), there are 13 elements deployed on board the ISS's U.S. Segment. The pre-flight service life prediction of 1 year for the BFEs is based upon performance engineering analysis of data collected during developmental testing that used a synthetic dust challenge. While this challenge is considered reasonable and conservative from a design perspective, an understanding of the actual filter loading is required to best manage the critical ISS Program resources. Thus testing was conducted on BFEs returned from the ISS to refine the service life prediction. Results from this testing and implications to ISS resource management are discussed. Recommendations for realizing significant savings to the ISS Program are presented.

  10. Tests and Calibration of the NIF Neutron Time of Flight Detectors

    International Nuclear Information System (INIS)

    Ali, Z.A.; Glebov, V.Yu.; Cruz, M.; Duffy, T.; Stoeckl, C.; Roberts, S.; Sangster, T.C.; Tommasini, R.; Throop, A; Moran, M.; Dauffy, L.; Horsefield, C.

    2008-01-01

    The National Ignition Facility (NIF) Neutron Time of Flight (NTOF) diagnostic will measure neutron yield and ion temperature in all NIF campaigns in DD, DT, and THD (D = deuterium, T = tritium, H = hydrogen) implosions. The NIF NTOF diagnostic is designed to measure neutron yield from 10 9 to 2 x 10 19 . The NTOF consists of several detectors of varying sensitivity located on the NIF at about 5 m and 20 m from the target. Production, testing, and calibration of the NIF NTOF detectors have begun at the Laboratory for Laser Energetics (LLE). Operational tests of the NTOF detectors were performed on several facilities including the OMEGA laser at LLE and the Titan laser at Lawrence Livermore National Laboratory (LLNL). Neutron calibrations were carried out on the OMEGA laser. Results of the NTOF detectors tests and calibration will be presented

  11. System identification of timber masonry walls using shaking table test

    Science.gov (United States)

    Roy, Timir B.; Guerreiro, Luis; Bagchi, Ashutosh

    2017-04-01

    Dynamic study is important in order to design, repair and rehabilitation of structures. It has played an important role in the behavior characterization of structures; such as: bridges, dams, high rise buildings etc. There had been substantial development in this area over the last few decades, especially in the field of dynamic identification techniques of structural systems. Frequency Domain Decomposition (FDD) and Time Domain Decomposition are most commonly used methods to identify modal parameters; such as: natural frequency, modal damping and mode shape. The focus of the present research is to study the dynamic characteristics of typical timber masonry walls commonly used in Portugal. For that purpose, a multi-storey structural prototype of such wall has been tested on a seismic shake table at the National Laboratory for Civil Engineering, Portugal (LNEC). Signal processing has been performed of the output response, which is collected from the shaking table experiment of the prototype using accelerometers. In the present work signal processing of the output response, based on the input response has been done in two ways: FDD and Stochastic Subspace Identification (SSI). In order to estimate the values of the modal parameters, algorithms for FDD are formulated and parametric functions for the SSI are computed. Finally, estimated values from both the methods are compared to measure the accuracy of both the techniques.

  12. Space Environment Testing of Photovoltaic Array Systems at NASA's Marshall Space Flight Center

    Science.gov (United States)

    Phillips, Brandon S.; Schneider, Todd A.; Vaughn, Jason A.; Wright, Kenneth H., Jr.

    2015-01-01

    To successfully operate a photovoltaic (PV) array system in space requires planning and testing to account for the effects of the space environment. It is critical to understand space environment interactions not only on the PV components, but also the array substrate materials, wiring harnesses, connectors, and protection circuitry (e.g. blocking diodes). Key elements of the space environment which must be accounted for in a PV system design include: Solar Photon Radiation, Charged Particle Radiation, Plasma, and Thermal Cycling. While solar photon radiation is central to generating power in PV systems, the complete spectrum includes short wavelength ultraviolet components, which photo-ionize materials, as well as long wavelength infrared which heat materials. High energy electron radiation has been demonstrated to significantly reduce the output power of III-V type PV cells; and proton radiation damages material surfaces - often impacting coverglasses and antireflective coatings. Plasma environments influence electrostatic charging of PV array materials, and must be understood to ensure that long duration arcs do not form and potentially destroy PV cells. Thermal cycling impacts all components on a PV array by inducing stresses due to thermal expansion and contraction. Given such demanding environments, and the complexity of structures and materials that form a PV array system, mission success can only be ensured through realistic testing in the laboratory. NASA's Marshall Space Flight Center has developed a broad space environment test capability to allow PV array designers and manufacturers to verify their system's integrity and avoid costly on-orbit failures. The Marshall Space Flight Center test capabilities are available to government, commercial, and university customers. Test solutions are tailored to meet the customer's needs, and can include performance assessments, such as flash testing in the case of PV cells.

  13. Flight test results for the Daedalus and Light Eagle human powered aircraft

    Science.gov (United States)

    Sullivan, R. Bryan; Zerweckh, Siegfried H.

    1988-01-01

    The results of the flight test program of the Daedalus and Light Eagle human powered aircraft in the winter of 1987/88 are given. The results from experiments exploring the Light Eagle's rigid body and structural dynamics are presented. The interactions of these dynamics with the autopilot design are investigated. Estimates of the power required to fly the Daedalus aircraft are detailed. The system of sensors, signal conditioning boards, and data acquisition equipment used to record the flight data is also described. In order to investigate the dynamics of the aircraft, flight test maneuvers were developed to yield maximum data quality from the point of view of estimating lateral and longitudinal stability derivatives. From this data, structural flexibility and unsteady aerodynamics have been modeled in an ad hoc manner and are used to augment the equations of motion with flexibility effects. Results of maneuvers that were flown are compared with the predictions from the flexibility model. To extend the ad hoc flexibility model, a fully flexible aeroelastic model has been developed. The model is unusual in the approximate equality of many structural natural frequencies and the importance of unsteady aerodynamic effects. the Gossamer Albatross. It is hypothesized that this inverse ground effect is caused by turbulence in the Earth's boundary layer. The diameters of the largest boundary layer eddies (which represent most of the turbulent kinetic energy) are proportional to altitude; thus, closer to the ground, the energy in the boundary layer becomes concentrated in eddies of smaller and smaller diameter. Eventually the eddies become sufficiently small (approximately 0.5 cm) that they trip the laminar boundary layer on the wing. As a result, a greater percentage of the wing area is covered with turbulent flow. Consequently the aircraft's drag and the pow er required both increase as the aircraft flies closer to the ground. The results of the flight test program are

  14. Development and test of a free-streaming readout chain for the CBM time of flight wall

    International Nuclear Information System (INIS)

    Loizeau, Pierre-Alain

    2014-01-01

    This thesis presents the development and test of a free-streaming readout chain for the Time of Flight (TOF) Wall of the Compressed Baryonic Matter (CBM) experiment. In order to contribute to the exploration of the phase diagram of strongly interacting matter, CBM aims at the measurement of rare probes, whose yields and phase space distributions are significantly influenced by their environment. Many of the possible signals, of which the antiprotons was investigated within this thesis, require an excellent Particle Identification (PID) and a new readout paradigm called free-streaming. In CBM, the PID for charged particles is provided by a TOF wall based on Multi-gap Resistive Plate Chambers (MRPC). Within the thesis, a central component of the TOF readout chain, the free-streaming ASIC-TDC, was evaluated and pushed from the prototype level to a close to final design, for which it could be demonstrated that it fulfill all the CBM requirements: resolution, rate capability and stability. Additionally, the CBM TOF software in the CBMROOT software framework was reorganized to merge the processing and analysis of real and simulated data. A data unpacker and a realistic digitizer were implemented with a common output data format. The digitizer was used to estimate the data rates and number of components in a free-streaming readout chain for the full wall.

  15. Modal testing and identification of a PWR fuel assembly

    International Nuclear Information System (INIS)

    Pisapia, S.; Collard, B.; Mori, V.; Bellizzi, S.

    2003-01-01

    This study aims at characterizing the vibratory behavior of a full-scale fuel assembly using an experimental approach. The effect of the assembly environment (air, stagnant water, and water under flow) is studied. The analysis of the test series shows that the vibratory behavior of full-scale fuel assembly is strongly nonlinear. An identification phase, based on temporal mean square criterion, allows us to obtain a nonlinear model representative of the first vibration mode of a fuel assembly. The selected class of models including damping and stiffness nonlinear terms is efficient in air, in stagnant water, and in water under flow. In all environments, the stiffness decreases with the displacement level and the damping increases with the velocity level. In the presence of water, the damping goes up and increases again with flowrate. (author)

  16. Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    Science.gov (United States)

    Anderson, Neil W; Buchan, Blake W; Riebe, Katherine M; Parsons, Lauren N; Gnacinski, Stacy; Ledeboer, Nathan A

    2012-03-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid method for the identification of bacteria. Factors that may alter protein profiles, including growth conditions and presence of exogenous substances, could hinder identification. Bacterial isolates identified by conventional methods were grown on various media and identified using the MALDI Biotyper (Bruker Daltonics, Billerica, MA) using a direct smear method and an acid extraction method. Specimens included 23 Pseudomonas isolates grown on blood agar, Pseudocel (CET), and MacConkey agar (MAC); 20 Staphylococcus isolates grown on blood agar, colistin-nalidixic acid agar (CNA), and mannitol salt agar (MSA); and 25 enteric isolates grown on blood agar, xylose lysine deoxycholate agar (XLD), Hektoen enteric agar (HE), salmonella-shigella agar (SS), and MAC. For Pseudomonas spp., the identification rate to genus using the direct method was 83% from blood, 78% from MAC, and 94% from CET. For Staphylococcus isolates, the identification rate to genus using the direct method was 95% from blood, 75% from CNA, and 95% from MSA. For enteric isolates, the identification rate to genus using the direct method was 100% from blood, 100% from MAC, 100% from XLD, 92% from HE, and 87% from SS. Extraction enhanced identification rates. The direct method of MALDI-TOF analysis of bacteria from selective and differential media yields identifications of varied confidence. Notably, Staphylococci spp. from CNA exhibit low identification rates. Extraction enhances identification rates and is recommended for colonies from this medium.

  17. Identification of significant features by the Global Mean Rank test.

    Science.gov (United States)

    Klammer, Martin; Dybowski, J Nikolaj; Hoffmann, Daniel; Schaab, Christoph

    2014-01-01

    With the introduction of omics-technologies such as transcriptomics and proteomics, numerous methods for the reliable identification of significantly regulated features (genes, proteins, etc.) have been developed. Experimental practice requires these tests to successfully deal with conditions such as small numbers of replicates, missing values, non-normally distributed expression levels, and non-identical distributions of features. With the MeanRank test we aimed at developing a test that performs robustly under these conditions, while favorably scaling with the number of replicates. The test proposed here is a global one-sample location test, which is based on the mean ranks across replicates, and internally estimates and controls the false discovery rate. Furthermore, missing data is accounted for without the need of imputation. In extensive simulations comparing MeanRank to other frequently used methods, we found that it performs well with small and large numbers of replicates, feature dependent variance between replicates, and variable regulation across features on simulation data and a recent two-color microarray spike-in dataset. The tests were then used to identify significant changes in the phosphoproteomes of cancer cells induced by the kinase inhibitors erlotinib and 3-MB-PP1 in two independently published mass spectrometry-based studies. MeanRank outperformed the other global rank-based methods applied in this study. Compared to the popular Significance Analysis of Microarrays and Linear Models for Microarray methods, MeanRank performed similar or better. Furthermore, MeanRank exhibits more consistent behavior regarding the degree of regulation and is robust against the choice of preprocessing methods. MeanRank does not require any imputation of missing values, is easy to understand, and yields results that are easy to interpret. The software implementing the algorithm is freely available for academic and commercial use.

  18. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry identification of large colony beta-hemolytic streptococci containing Lancefield groups A, C, and G.

    Science.gov (United States)

    Jensen, Christian Salgård; Dam-Nielsen, Casper; Arpi, Magnus

    2015-08-01

    The aim of this study was to investigate whether large colony beta-hemolytic streptococci containing Lancefield groups A, C, and G can be adequately identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-ToF). Previous studies show varying results, with an identification rate from below 50% to 100%. Large colony beta-hemolytic streptococci containing Lancefield groups A, C, and G isolated from blood cultures between January 1, 2007 and May 1, 2012 were included in the study. Isolates were identified to the species level using a combination of phenotypic characteristics and 16s rRNA sequencing. The isolates were subjected to MALDI-ToF analysis. We used a two-stage approach starting with the direct method. If no valid result was obtained we proceeded to an extraction protocol. Scores above 2 were considered valid identification at the species level. A total of 97 Streptococcus pyogenes, 133 Streptococcus dysgalactiae, and 2 Streptococcus canis isolates were tested; 94%, 66%, and 100% of S. pyogenes, S. dysgalactiae, and S. canis, respectively, were correctly identified by MALDI-ToF. In most instances when the isolates were not identified by MALDI-ToF this was because MALDI-ToF was unable to differentiate between S. pyogenes and S. dysgalactiae. By removing two S. pyogenes reference spectra from the MALDI-ToF database the proportion of correctly identified isolates increased to 96% overall. MALDI-ToF is a promising method for discriminating between S. dysgalactiae, S. canis, and S. equi, although more strains need to be tested to clarify this.

  19. Analysis of Droplet Size during the Ice Accumulation Phase Of Flight Testing

    Science.gov (United States)

    Miller, Eric James

    2004-01-01

    There are numerous hazards associated with air travel. One of the most serious dangers to the pilot and passengers safety is the result of flying into conditions which are conducive to the formation of ice on the surface of an aircraft. Being a pilot myself I am very aware of the dangers that Icing can pose and the effects it can have on an airplane. A couple of the missions of the Icing branch is to make flying safer with more research to increase our knowledge of how ice effects the aerodynamics of an airfoil, and to increase are knowledge of the weather for better forecasting. The Icing Branch uses three different tools to determine the aerodynamic affects that icing has on a wing. The Icing research tunnel is an efficient way to test various airfoils in a controlled setting. To make sure the data received from the wind tunnel is accurate the Icing branch conducts real flight tests with the DHC-6 Twin Otter. This makes sure that the methods used in the wind tunnel accurately model what happens on the actual aircraft. These two tools are also compared to the LEWICE code which is a program that models the ice shape that would be formed on an airfoil in the particular weather conditions that are input by the user. One benefit of LEWICE is that it is a lot cheaper to run than the wind tunnel or flight tests which make it a nice tool for engineers designing aircraft that don t have the money to spend on icing research. Using all three of these tools is a way to cross check the data received from one and check it against the other two. industries, but it is also looked at by weather analysts who are trying to improve forecasting methods. The best way to avoid the troubles of icing encounters is to not go into it in the first place. By looking over the flight data the analyst can determine which conditions will most likely lead to an icing encounter and then this information will aid forecasters when briefing the pilots on the weather conditions. am looking at the

  20. Force Limiting Vibration Tests Evaluated from both Ground Acoustic Tests and FEM Simulations of a Flight Like Vehicle System Assembly

    Science.gov (United States)

    Smith, Andrew; LaVerde, Bruce; Waldon, James; Hunt, Ron

    2014-01-01

    Marshall Space Flight Center has conducted a series of ground acoustic tests with the dual goals of informing analytical judgment, and validating analytical methods when estimating vibroacoustic responses of launch vehicle subsystems. The process of repeatedly correlating finite element-simulated responses with test-measured responses has assisted in the development of best practices for modeling and post-processing. In recent work, force transducers were integrated to measure interface forces at the base of avionics box equipment. Other force data was indirectly measured using strain gauges. The combination of these direct and indirect force measurements has been used to support and illustrate the advantages of implementing the Force Limiting approach for equipment qualification tests. The comparison of force response from integrated system level tests to measurements at the same locations during component level vibration tests provides an excellent illustration. A second comparison of the measured response cases from the system level acoustic tests to finite element simulations has also produced some principles for assessing the suitability of Finite Element Models (FEMs) for making vibroacoustics estimates. The results indicate that when FEM models are employed to guide force limiting choices, they should include sufficient detail to represent the apparent mass of the system in the frequency range of interest.

  1. ATON (Autonomous Terrain-based Optical Navigation) for exploration missions: recent flight test results

    Science.gov (United States)

    Theil, S.; Ammann, N.; Andert, F.; Franz, T.; Krüger, H.; Lehner, H.; Lingenauber, M.; Lüdtke, D.; Maass, B.; Paproth, C.; Wohlfeil, J.

    2018-03-01

    Since 2010 the German Aerospace Center is working on the project Autonomous Terrain-based Optical Navigation (ATON). Its objective is the development of technologies which allow autonomous navigation of spacecraft in orbit around and during landing on celestial bodies like the Moon, planets, asteroids and comets. The project developed different image processing techniques and optical navigation methods as well as sensor data fusion. The setup—which is applicable to many exploration missions—consists of an inertial measurement unit, a laser altimeter, a star tracker and one or multiple navigation cameras. In the past years, several milestones have been achieved. It started with the setup of a simulation environment including the detailed simulation of camera images. This was continued by hardware-in-the-loop tests in the Testbed for Robotic Optical Navigation (TRON) where images were generated by real cameras in a simulated downscaled lunar landing scene. Data were recorded in helicopter flight tests and post-processed in real-time to increase maturity of the algorithms and to optimize the software. Recently, two more milestones have been achieved. In late 2016, the whole navigation system setup was flying on an unmanned helicopter while processing all sensor information onboard in real time. For the latest milestone the navigation system was tested in closed-loop on the unmanned helicopter. For that purpose the ATON navigation system provided the navigation state for the guidance and control of the unmanned helicopter replacing the GPS-based standard navigation system. The paper will give an introduction to the ATON project and its concept. The methods and algorithms of ATON are briefly described. The flight test results of the latest two milestones are presented and discussed.

  2. TRISTAR I: Evaluation Methods for Testing Head-Up Display (HUD) Flight Symbology

    National Research Council Canada - National Science Library

    Newman, R

    1995-01-01

    A piloted head up display (HUD) flight symbology study (TRISTAR) measuring pilot task performance was conducted at the NASA Ames Research Center by the Tri-Service Flight Symbology Working Group (FSWO...

  3. Probing Aircraft Flight Test Hazard Mitigation for the Alternative Fuel Effects on Contrails & Cruise Emissions (ACCESS) Research Team

    Science.gov (United States)

    Kelly, Michael J.

    2013-01-01

    The Alternative Fuel Effects on Contrails & Cruise Emissions (ACCESS) Project Integration Manager requested in July 2012 that the NASA Engineering and Safety Center (NESC) form a team to independently assess aircraft structural failure hazards associated with the ACCESS experiment and to identify potential flight test hazard mitigations to ensure flight safety. The ACCESS Project Integration Manager subsequently requested that the assessment scope be focused predominantly on structural failure risks to the aircraft empennage raft empennage.

  4. Control and Non-Payload Communications Generation 1 Prototype Radio Flight Test Report

    Science.gov (United States)

    Shalkhauser, Kurt A.; Young, Daniel P.; Bretmersky, Steven C.; Ishac, Joseph A.; Walker, Steven H.; Griner, James H.; Kachmar, Brian A.

    2014-01-01

    regularity of flight. Only recently has radiofrequency (RF) spectrum been allocated by the International Telecommunications Union specifically for commercial UA C2, LOS communication (L-Band: 960 to 1164 MHz, and C-Band: 5030 to 5091 MHz). The safe and efficient integration of UA into the NAS requires the use of protected RF spectrum allocations and a new data communications system that is both secure and scalable to accommodate the potential growth of these new aircraft. Data communications for UA-referred to as control and non-payload communications (CNPC)-will be used to exchange information between a UA and a ground station (GS) to ensure safe, reliable, and effective UA flight operation. The focus of this effort is on validating and allocating new RF spectrum and data link communications to enable civil UA integration into the NAS. Through a cost-sharing cooperative agreement with Rockwell Collins, Inc., the NASA Glenn Research Center is exploring and performing the necessary development steps to realize a prototype UA CNPC system. These activities include investigating signal waveforms and access techniques, developing representative CNPC radio hardware, and executing relevant testing and validation activities. There is no intent to manufacture the CNPC end product, rather the goals are to study, demonstrate, and validate a typical CNPC system that will allow safe and efficient communications within the L-Band and C-Band spectrum allocations. The system is addressing initial "seed" requirements from RTCA, Inc., Special Committee 203 (SC-203) and is on a path to Federal Aviation Administration certification. This report provides results from the flight testing campaign of the Rockwell Collins Generation 1 prototype radio, referred hereafter as the "radio." The radio sets operate within the 960- to 977-MHz frequency band with both air and ground radios using identical hardware. Flight tests involved one aircraft and one GS. Results include discussion of aircraft flight

  5. Verification of protein biomarker specificity for the identification of biological stains by quadrupole time-of-flight mass spectrometry.

    Science.gov (United States)

    Legg, Kevin M; Powell, Roger; Reisdorph, Nichole; Reisdorph, Rick; Danielson, Phillip B

    2017-03-01

    Advances in proteomics technology over the past decade offer forensic serologists a greatly improved opportunity to accurately characterize the tissue source from which a DNA profile has been developed. Such information can provide critical context to evidence and can help to prioritize downstream DNA analyses. Previous proteome studies compiled panels of "candidate biomarkers" specific to each of five body fluids (i.e., peripheral blood, vaginal/menstrual fluid, seminal fluid, urine, and saliva). Here, a multiplex quadrupole time-of-flight mass spectrometry assay has been developed in order to verify the tissue/body fluid specificity the 23 protein biomarkers that comprise these panels and the consistency with which they can be detected across a sample population of 50 humans. Single-source samples of these human body fluids were accurately identified by the detection of one or more high-specificity biomarkers. Recovery of body fluid samples from a variety of substrates did not impede accurate characterization and, of the potential inhibitors assayed, only chewing tobacco juice appeared to preclude the identification of a target body fluid. Using a series of 2-component mixtures of human body fluids, the multiplex assay accurately identified both components in a single-pass. Only in the case of saliva and peripheral blood did matrix effects appear to impede the detection of salivary proteins. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Identification and preclinical testing of novel antiepileptic compounds.

    Science.gov (United States)

    Meldrum, B S

    1997-01-01

    Procedures for identifying novel antiepileptic drugs (AEDs) are changing and need to change more. Widespread reliance on two primary screens has led to the identification of novel compounds that resemble either phenytoin (suppressing high-frequency repetitive firing in cultured neurons and prolonging inactivation of voltage-dependent sodium channels identified by the maximal electroshock test) or benzodiazepines (potentiating the inhibitory effect of gamma-aminobutyric acid (GABA), identified by the threshold pentylenetetrazol test). Advances in molecular neurobiology have identified specific molecular targets (subunits of ion channels, neurotransmitter receptors, and transporters) and have made them available in a form permitting high-throughput screening. AEDs can be designed to interact with specific sites on the target molecules. Alternatively, the molecular screens can be used to identify active components in natural products, including folk remedies. Preclinical in vivo screens can be improved by using animals with genetic or acquired epilepsies that have similar modifications in the properties of the target molecules as do human epilepsy syndromes. Future work is likely to define molecular targets for AEDs that will block or reverse chronic epileptogenesis.

  7. Optimal Control Allocation with Load Sensor Feedback for Active Load Suppression, Flight-Test Performance

    Science.gov (United States)

    Miller, Christopher J.; Goodrick, Dan

    2017-01-01

    The problem of control command and maneuver induced structural loads is an important aspect of any control system design. The aircraft structure and the control architecture must be designed to achieve desired piloted control responses while limiting the imparted structural loads. The classical approach is to utilize high structural margins, restrict control surface commands to a limited set of analyzed combinations, and train pilots to follow procedural maneuvering limitations. With recent advances in structural sensing and the continued desire to improve safety and vehicle fuel efficiency, it is both possible and desirable to develop control architectures that enable lighter vehicle weights while maintaining and improving protection against structural damage. An optimal control technique has been explored and shown to achieve desirable vehicle control performance while limiting sensed structural loads to specified values. This technique has been implemented and flown on the National Aeronautics and Space Administration Full-scale Advanced Systems Testbed aircraft. The flight tests illustrate that the approach achieves the desired performance and show promising potential benefits. The flights also uncovered some important issues that will need to be addressed for production application.

  8. Small UAV Automatic Ground Collision Avoidance System Design Considerations and Flight Test Results

    Science.gov (United States)

    Sorokowski, Paul; Skoog, Mark; Burrows, Scott; Thomas, SaraKatie

    2015-01-01

    The National Aeronautics and Space Administration (NASA) Armstrong Flight Research Center Small Unmanned Aerial Vehicle (SUAV) Automatic Ground Collision Avoidance System (Auto GCAS) project demonstrated several important collision avoidance technologies. First, the SUAV Auto GCAS design included capabilities to take advantage of terrain avoidance maneuvers flying turns to either side as well as straight over terrain. Second, the design also included innovative digital elevation model (DEM) scanning methods. The combination of multi-trajectory options and new scanning methods demonstrated the ability to reduce the nuisance potential of the SUAV while maintaining robust terrain avoidance. Third, the Auto GCAS algorithms were hosted on the processor inside a smartphone, providing a lightweight hardware configuration for use in either the ground control station or on board the test aircraft. Finally, compression of DEM data for the entire Earth and successful hosting of that data on the smartphone was demonstrated. The SUAV Auto GCAS project demonstrated that together these methods and technologies have the potential to dramatically reduce the number of controlled flight into terrain mishaps across a wide range of aviation platforms with similar capabilities including UAVs, general aviation aircraft, helicopters, and model aircraft.

  9. Trajectory Reconstruction and Uncertainty Analysis Using Mars Science Laboratory Pre-Flight Scale Model Aeroballistic Testing

    Science.gov (United States)

    Lugo, Rafael A.; Tolson, Robert H.; Schoenenberger, Mark

    2013-01-01

    As part of the Mars Science Laboratory (MSL) trajectory reconstruction effort at NASA Langley Research Center, free-flight aeroballistic experiments of instrumented MSL scale models was conducted at Aberdeen Proving Ground in Maryland. The models carried an inertial measurement unit (IMU) and a flush air data system (FADS) similar to the MSL Entry Atmospheric Data System (MEADS) that provided data types similar to those from the MSL entry. Multiple sources of redundant data were available, including tracking radar and on-board magnetometers. These experimental data enabled the testing and validation of the various tools and methodologies that will be used for MSL trajectory reconstruction. The aerodynamic parameters Mach number, angle of attack, and sideslip angle were estimated using minimum variance with a priori to combine the pressure data and pre-flight computational fluid dynamics (CFD) data. Both linear and non-linear pressure model terms were also estimated for each pressure transducer as a measure of the errors introduced by CFD and transducer calibration. Parameter uncertainties were estimated using a "consider parameters" approach.

  10. Assessing Arboreal Adaptations of Bird Antecedents: Testing the Ecological Setting of the Origin of the Avian Flight Stroke

    Science.gov (United States)

    Dececchi, T. Alexander; Larsson, Hans C. E.

    2011-01-01

    The origin of avian flight is a classic macroevolutionary transition with research spanning over a century. Two competing models explaining this locomotory transition have been discussed for decades: ground up versus trees down. Although it is impossible to directly test either of these theories, it is possible to test one of the requirements for the trees-down model, that of an arboreal paravian. We test for arboreality in non-avian theropods and early birds with comparisons to extant avian, mammalian, and reptilian scansors and climbers using a comprehensive set of morphological characters. Non-avian theropods, including the small, feathered deinonychosaurs, and Archaeopteryx, consistently and significantly cluster with fully terrestrial extant mammals and ground-based birds, such as ratites. Basal birds, more advanced than Archaeopteryx, cluster with extant perching ground-foraging birds. Evolutionary trends immediately prior to the origin of birds indicate skeletal adaptations opposite that expected for arboreal climbers. Results reject an arboreal capacity for the avian stem lineage, thus lending no support for the trees-down model. Support for a fully terrestrial ecology and origin of the avian flight stroke has broad implications for the origin of powered flight for this clade. A terrestrial origin for the avian flight stroke challenges the need for an intermediate gliding phase, presents the best resolved series of the evolution of vertebrate powered flight, and may differ fundamentally from the origin of bat and pterosaur flight, whose antecedents have been postulated to have been arboreal and gliding. PMID:21857918

  11. Assessing arboreal adaptations of bird antecedents: testing the ecological setting of the origin of the avian flight stroke.

    Directory of Open Access Journals (Sweden)

    T Alexander Dececchi

    Full Text Available The origin of avian flight is a classic macroevolutionary transition with research spanning over a century. Two competing models explaining this locomotory transition have been discussed for decades: ground up versus trees down. Although it is impossible to directly test either of these theories, it is possible to test one of the requirements for the trees-down model, that of an arboreal paravian. We test for arboreality in non-avian theropods and early birds with comparisons to extant avian, mammalian, and reptilian scansors and climbers using a comprehensive set of morphological characters. Non-avian theropods, including the small, feathered deinonychosaurs, and Archaeopteryx, consistently and significantly cluster with fully terrestrial extant mammals and ground-based birds, such as ratites. Basal birds, more advanced than Archaeopteryx, cluster with extant perching ground-foraging birds. Evolutionary trends immediately prior to the origin of birds indicate skeletal adaptations opposite that expected for arboreal climbers. Results reject an arboreal capacity for the avian stem lineage, thus lending no support for the trees-down model. Support for a fully terrestrial ecology and origin of the avian flight stroke has broad implications for the origin of powered flight for this clade. A terrestrial origin for the avian flight stroke challenges the need for an intermediate gliding phase, presents the best resolved series of the evolution of vertebrate powered flight, and may differ fundamentally from the origin of bat and pterosaur flight, whose antecedents have been postulated to have been arboreal and gliding.

  12. Immunotoxicity and genotoxicity testing for in-flight experiments under microgravity

    Science.gov (United States)

    Hansen, Peter-Diedrich; Hansen, Peter-Diedrich; Unruh, Eckehardt

    Life Sciences as Related to Space (F) Influence of Spaceflight Environment on Biological Systems (F44) Immunotoxicity and genotoxicity testing for In-flight experiments under microgravity Sensing approaches for ecosystem and human health Author: Peter D. Hansen Technische Universit¨t Berlin, Faculty VI - Planen, Bauen, Umwelt, a Institute for Ecological Research and Technology, Department for Ecotoxicology, Berlin, Germany Peter-diedrich.hansen@tu-berlin.de Eckehardt Unruh Technische Universit¨t Berlin, Faculty VI - Planen, Bauen, Umwelt, Institute a for Ecological Research and Technology, Department for Ecotoxicology, Berlin, Germany An immune response by mussel hemocytes is the selective reaction to particles which are identified as foreign by its immune system shown by phagocytosis. Phagocytotic activity is based on the chemotaxis and adhesion, ingestion and phagosome formation. The attachment at the surface of the hemocytes and consequently the uptake of the particles or bacteria can be directly quantified in the format of a fluorescent assay. Another relevant endpoint of phagocytosis is oxidative burst measured by luminescence. Phagocytosis-related production of ROS will be stimulated with opsonised zymosan. The hemocytes will be stored frozen at -80oC and reconstituted in-flight for the experiment. The assay system of the TRIPLELUX-B Experiment has been performed with a well-defined quantification and evaluation of the immune function phagocytosis. The indicator cells are the hemocytes of blue mussels (Mytilus edulis). The signals of the immuno cellular responses are translated into luminescence as a rapid optical reporter system. The results expected will determine whether the observed responses are caused by microgravity and/or radiation (change in permeability, endpoints in genotoxicity: DNA unwinding). The samples for genotoxicity will be processed after returning to earth. The immune system of invertebrates has not been studied so far in space. The

  13. Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Aspergillus, Scedosporium, and Fusarium spp. in the Australian Clinical Setting.

    Science.gov (United States)

    Sleiman, Sue; Halliday, Catriona L; Chapman, Belinda; Brown, Mitchell; Nitschke, Joanne; Lau, Anna F; Chen, Sharon C-A

    2016-08-01

    We developed an Australian database for the identification of Aspergillus, Scedosporium, and Fusarium species (n = 28) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In a challenge against 117 isolates, species identification significantly improved when the in-house-built database was combined with the Bruker Filamentous Fungi Library compared with that for the Bruker library alone (Aspergillus, 93% versus 69%; Fusarium, 84% versus 42%; and Scedosporium, 94% versus 18%, respectively). Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  14. Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.

    Directory of Open Access Journals (Sweden)

    Bernard La Scola

    Full Text Available BACKGROUND: With long delays observed between sampling and availability of results, the usefulness of blood cultures in the context of emergency infectious diseases has recently been questioned. Among methods that allow quicker bacterial identification from growing colonies, matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF mass spectrometry was demonstrated to accurately identify bacteria routinely isolated in a clinical biology laboratory. In order to speed up the identification process, in the present work we attempted bacterial identification directly from blood culture bottles detected positive by the automate. METHODOLOGY/PRINCIPAL FINDINGS: We prospectively analysed routine MALDI-TOF identification of bacteria detected in blood culture by two different protocols involving successive centrifugations and then lysis by trifluoroacetic acid or formic acid. Of the 562 blood culture broths detected as positive by the automate and containing one bacterial species, 370 (66% were correctly identified. Changing the protocol from trifluoroacetic acid to formic acid improved identification of Staphylococci, and overall correct identification increased from 59% to 76%. Lack of identification was observed mostly with viridans streptococci, and only one false positive was observed. In the 22 positive blood culture broths that contained two or more different species, only one of the species was identified in 18 samples, no species were identified in two samples and false species identifications were obtained in two cases. The positive predictive value of bacterial identification using this procedure was 99.2%. CONCLUSIONS/SIGNIFICANCE: MALDI-TOF MS is an efficient method for direct routine identification of bacterial isolates in blood culture, with the exception of polymicrobial samples and viridans streptococci. It may replace routine identification performed on colonies, provided improvement for the specificity of blood culture

  15. Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.

    Science.gov (United States)

    La Scola, Bernard; Raoult, Didier

    2009-11-25

    With long delays observed between sampling and availability of results, the usefulness of blood cultures in the context of emergency infectious diseases has recently been questioned. Among methods that allow quicker bacterial identification from growing colonies, matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry was demonstrated to accurately identify bacteria routinely isolated in a clinical biology laboratory. In order to speed up the identification process, in the present work we attempted bacterial identification directly from blood culture bottles detected positive by the automate. We prospectively analysed routine MALDI-TOF identification of bacteria detected in blood culture by two different protocols involving successive centrifugations and then lysis by trifluoroacetic acid or formic acid. Of the 562 blood culture broths detected as positive by the automate and containing one bacterial species, 370 (66%) were correctly identified. Changing the protocol from trifluoroacetic acid to formic acid improved identification of Staphylococci, and overall correct identification increased from 59% to 76%. Lack of identification was observed mostly with viridans streptococci, and only one false positive was observed. In the 22 positive blood culture broths that contained two or more different species, only one of the species was identified in 18 samples, no species were identified in two samples and false species identifications were obtained in two cases. The positive predictive value of bacterial identification using this procedure was 99.2%. MALDI-TOF MS is an efficient method for direct routine identification of bacterial isolates in blood culture, with the exception of polymicrobial samples and viridans streptococci. It may replace routine identification performed on colonies, provided improvement for the specificity of blood culture broths growing viridans streptococci is obtained in the near future.

  16. Summary of the First High-Altitude, Supersonic Flight Dynamics Test for the Low-Density Supersonic Decelerator Project

    Science.gov (United States)

    Clark, Ian G.; Adler, Mark; Manning, Rob

    2015-01-01

    NASA's Low-Density Supersonic Decelerator Project is developing and testing the next generation of supersonic aerodynamic decelerators for planetary entry. A key element of that development is the testing of full-scale articles in conditions relevant to their intended use, primarily the tenuous Mars atmosphere. To achieve this testing, the LDSD project developed a test architecture similar to that used by the Viking Project in the early 1970's for the qualification of their supersonic parachute. A large, helium filled scientific balloon is used to hoist a 4.7 m blunt body test vehicle to an altitude of approximately 32 kilometers. The test vehicle is released from the balloon, spun up for gyroscopic stability, and accelerated to over four times the speed of sound and an altitude of 50 kilometers using a large solid rocket motor. Once at those conditions, the vehicle is despun and the test period begins. The first flight of this architecture occurred on June 28th of 2014. Though primarily a shake out flight of the new test system, the flight was also able to achieve an early test of two of the LDSD technologies, a large 6 m diameter Supersonic Inflatable Aerodynamic Decelerator (SIAD) and a large, 30.5 m nominal diameter supersonic parachute. This paper summarizes this first flight.

  17. Optimal design of tests for heat exchanger fouling identification

    International Nuclear Information System (INIS)

    Palmer, Kyle A.; Hale, William T.; Such, Kyle D.; Shea, Brian R.; Bollas, George M.

    2016-01-01

    Highlights: • Built-in test design that optimizes the information extractable from the said test. • Method minimizes the covariance of a fault with system uncertainty. • Method applied for the identification and quantification of heat exchanger fouling. • Heat exchanger fouling is identifiable despite the uncertainty in inputs and states. - Graphical Abstract: - Abstract: Particulate fouling in plate fin heat exchangers of aircraft environmental control systems is a recurring issue in environments rich in foreign object debris. Heat exchanger fouling detection, in terms of quantification of its severity, is critical for aircraft maintenance scheduling and safe operation. In this work, we focus on methods for offline fouling detection during aircraft ground handling, where the allowable variability range of admissible inputs is wider. We explore methods of optimal experimental design to estimate heat exchanger inputs and input trajectories that maximize the identifiability of fouling. In particular, we present a methodology in which D-optimality is used as a criterion for statistically significant inference of heat exchanger fouling in uncertain environments. The optimal tests are designed on the basis of a heat exchanger model of the inherent mass, energy and momentum balances, validated against literature data. The model is then used to infer sensitivities of the heat exchanger outputs with respect to fouling metrics and maximize them by manipulating input trajectories; thus enhancing the accuracy in quantifying the fouling extent. The proposed methodology is evaluated with statistical indices of the confidence in estimating thermal fouling resistance at uncertain operating conditions, explored in a series of case studies.

  18. Flight test operations using an F-106B research airplane modified with a wing leading-edge vortex flap

    Science.gov (United States)

    Dicarlo, Daniel J.; Brown, Philip W.; Hallissy, James B.

    1992-01-01

    Flight tests of an F-106B aircraft equipped with a leading-edge vortex flap, which represented the culmination of a research effort to examine the effectiveness of the flap, were conducted at the NASA Langley Research Center. The purpose of the flight tests was to establish a data base on the use of a wing leading-edge vortex flap as a means to validate the design and analysis methods associated with the development of such a vortical flow-control concept. The overall experiment included: refinements of the design codes for vortex flaps; numerous wind tunnel entries to aid in verifying design codes and determining basic aerodynamic characteristics; design and fabrication of the flaps, structural modifications to the wing tip and leading edges of the test aircraft; development and installation of an aircraft research instrumentation system, including wing and flap surface pressure measurements and selected structural loads measurements; ground-based simulation to assess flying qualities; and finally, flight testing. This paper reviews the operational aspects associated with the flight experiment, which includes a description of modifications to the research airplane, the overall flight test procedures, and problems encountered. Selected research results are also presented to illustrate the accomplishments of the research effort.

  19. Comparison of analysis and flight test data for a drone aircraft with active flutter suppression

    Science.gov (United States)

    Newsom, J. R.; Pototzky, A. S.

    1981-01-01

    A drone aircraft equipped with an active flutter suppression system is considered with emphasis on the comparison of modal dampings and frequencies as a function of Mach number. Results are presented for both symmetric and antisymmetric motion with flutter suppression off. Only symmetric results are given for flutter suppression on. Frequency response functions of the vehicle are presented from both flight test data and analysis. The analysis correlation is improved by using an empirical aerodynamic correction factor which is proportional to the ratio of experimental to analytical steady-state lift curve slope. The mathematical models are included and existing analytical techniques are described as well as an alternative analytical technique for obtaining closed-loop results.

  20. A method for calibration and test of the time-of-flight detectors for DELPHI

    International Nuclear Information System (INIS)

    Benlloch, J.M.; Castillo, M.V.; Ferrer, A.; Fuster, J.; Higon, E.; Lozano, J.; Salt, J.; Sanchez, E.; Sanchis, E.; Cuevas, J.

    1990-01-01

    We describe a method for calibration and test of large-area TOF counters using cosmic radiation. We applied the method to the time-of-flight system of the DELPHI detector at the LEP e + e - storage ring, made of scintillation (NE110) counters (20x350 cm 2 ). The photomultipliers used (EMI 9902KB) reach an average gain of 5x10 8 at 1700 V and the time resolution achieved is 1.2 ns. Using this method we measured the counter efficiencies as a function of the position; we obtained 135 cm for the effective attenuation length and 40 photoelectrons for a minimum-ionizing particle crossing the center of the counter. (orig.)

  1. Testing of Full Scale Flight Qualified Kevlar Composite Overwrapped Pressure Vessels

    Science.gov (United States)

    Greene, Nathanael; Saulsberry, Regor; Yoder, Tommy; Forsyth, Brad; Thesken, John; Phoenix, Leigh

    2007-01-01

    Many decades ago NASA identified a need for low-mass pressure vessels for carrying various fluids aboard rockets, spacecraft, and satellites. A pressure vessel design known as the composite overwrapped pressure vessel (COPV) was identified to provide a weight savings over traditional single-material pressure vessels typically made of metal and this technology has been in use for space flight applications since the 1970's. A typical vessel design consisted of a thin liner material, typically a metal, overwrapped with a continuous fiber yarn impregnated with epoxy. Most designs were such that the overwrapped fiber would carry a majority of load at normal operating pressures. The weight advantage for a COPV versus a traditional singlematerial pressure vessel contributed to widespread use of COPVs by NASA, the military, and industry. This technology is currently used for personal breathing supply storage, fuel storage for auto and mass transport vehicles and for various space flight and aircraft applications. The NASA Engineering and Safety Center (NESC) was recently asked to review the operation of Kevlar 2 and carbon COPVs to ensure they are safely operated on NASA space flight vehicles. A request was made to evaluate the life remaining on the Kevlar COPVs used on the Space Shuttle for helium and nitrogen storage. This paper provides a review of Kevlar COPV testing relevant to the NESC assessment. Also discussed are some key findings, observations, and recommendations that may be applicable to the COPV user community. Questions raised during the investigations have revealed the need for testing to better understand the stress rupture life and age life of COPVs. The focus of this paper is to describe burst testing of Kevlar COPVs that has been completed as a part of an the effort to evaluate the effects of ageing and shelf life on full scale COPVs. The test articles evaluated in this discussion had a diameter of 22 inches for S/N 014 and 40 inches for S/N 011. The

  2. Development and flight test of metal-lined CFRP cryogenic tank for reusable rocket

    Science.gov (United States)

    Higuchi, Ken; Takeuchi, Shinsuke; Sato, Eiichi; Naruo, Yoshihiro; Inatani, Yoshifumi; Namiki, Fumiharu; Tanaka, Kohtaro; Watabe, Yoko

    2005-07-01

    A cryogenic tank made of carbon fiber reinforced plastic (CFRP) shell with aluminum thin liner has been designed as a liquid hydrogen (LH2) tank for an ISAS reusable launch vehicle, and the function of it has been proven by repeated flights onboard the test vehicle called reusable vehicle testing (RVT) in October 2003. The liquid hydrogen tank has to be a pressure vessel, because the fuel of the engine of the test vehicle is supplied by fuel pressure. The pressure vessel of a combination of the outer shell of CFRP for strength element at a cryogenic temperature and the inner liner of aluminum for gas barrier has shown excellent weight merit for this purpose. Interfaces such as tank outline shape, bulk capacity, maximum expected operating pressure (MEOP), thermal insulation, pipe arrangement, and measurement of data are also designed to be ready onboard. This research has many aims, not only development of reusable cryogenic composite tank but also the demonstration of repeated operation including thermal cycle and stress cycle, familiarization with test techniques of operation of cryogenic composite tanks, and the accumulation of data for future design of tanks, vehicle structures, safety evaluation, and total operation systems.

  3. UAS Integration in the NAS Project: Integrated Test and Evaluation (IT&E) Flight Test 3. Revision E

    Science.gov (United States)

    Marston, Michael

    2015-01-01

    The desire and ability to fly Unmanned Aircraft Systems (UAS) in the National Airspace System (NAS) is of increasing urgency. The application of unmanned aircraft to perform national security, defense, scientific, and emergency management are driving the critical need for less restrictive access by UAS to the NAS. UAS represent a new capability that will provide a variety of services in the government (public) and commercial (civil) aviation sectors. The growth of this potential industry has not yet been realized due to the lack of a common understanding of what is required to safely operate UAS in the NAS. NASA's UAS Integration into the NAS Project is conducting research in the areas of Separation Assurance/Sense and Avoid Interoperability, Human Systems Integration (HSI), and Communication to support reducing the barriers of UAS access to the NAS. This research is broken into two research themes namely, UAS Integration and Test Infrastructure. UAS Integration focuses on airspace integration procedures and performance standards to enable UAS integration in the air transportation system, covering Sense and Avoid (SAA) performance standards, command and control performance standards, and human systems integration. The focus of Test Infrastructure is to enable development and validation of airspace integration procedures and performance standards, including the integrated test and evaluation. In support of the integrated test and evaluation efforts, the Project will develop an adaptable, scalable, and schedulable relevant test environment capable of evaluating concepts and technologies for unmanned aircraft systems to safely operate in the NAS. To accomplish this task, the Project will conduct a series of Human-in-the-Loop and Flight Test activities that integrate key concepts, technologies and/or procedures in a relevant air traffic environment. Each of the integrated events will build on the technical achievements, fidelity and complexity of the previous tests and

  4. Identification of an abnormal beryllium lymphocyte proliferation test

    International Nuclear Information System (INIS)

    Frome, Edward L.; Newman, Lee S.; Cragle, Donna L.; Colyer, Shirley P.; Wambach, Paul F.

    2003-01-01

    The potential hazards from exposure to beryllium or beryllium compounds in the workplace were first reported in the 1930s. The tritiated thymidine beryllium lymphocyte proliferation test (BeLPT) is an in vitro blood test that is widely used to screen beryllium exposed workers in the nuclear industry for sensitivity to beryllium. The clinical significance of the BeLPT was described and a standard protocol was developed in the late 1980s. Cell proliferation is measured by the incorporation of tritiated thymidine into dividing cells on two culture dates and using three concentrations of beryllium sulfate. Results are expressed as a 'stimulation index' (SI) which is the ratio of the amount of tritiated thymidine (measured by beta counts) in the simulated cells divided by the counts for the unstimulated cells on the same culture day. Several statistical methods for use in the routine analysis of the BeLPT were proposed in the early 1990s. The least absolute values (LAV) method was recommended for routine analysis of the BeLPT. This report further evaluates the LAV method using new data, and proposes a new method for identification of an abnormal or borderline test. This new statistical-biological positive (SBP) method reflects the clinical judgment that: (i) at least two SIs show a 'positive' response to beryllium; and (ii) that the maximum of the six SIs must exceed a cut-point that is determined from a reference data set of normal individuals whose blood has been tested by the same method in the same serum. The new data is from the Y-12 National Security Complex in Oak Ridge (Y-12) and consists of 1080 workers and 33 non-exposed control BeLPTs (all tested in the same serum). Graphical results are presented to explain the statistical method, and the new SBP method is applied to the Y-12 group. The true positive rate and specificity of the new method were estimated to be 86% and 97%, respectively. An electronic notebook that is accessible via the Internet was used in

  5. Flight Testing of the Space Launch System (SLS) Adaptive Augmenting Control (AAC) Algorithm on an F/A-18

    Science.gov (United States)

    Dennehy, Cornelius J.; VanZwieten, Tannen S.; Hanson, Curtis E.; Wall, John H.; Miller, Chris J.; Gilligan, Eric T.; Orr, Jeb S.

    2014-01-01

    The Marshall Space Flight Center (MSFC) Flight Mechanics and Analysis Division developed an adaptive augmenting control (AAC) algorithm for launch vehicles that improves robustness and performance on an as-needed basis by adapting a classical control algorithm to unexpected environments or variations in vehicle dynamics. This was baselined as part of the Space Launch System (SLS) flight control system. The NASA Engineering and Safety Center (NESC) was asked to partner with the SLS Program and the Space Technology Mission Directorate (STMD) Game Changing Development Program (GCDP) to flight test the AAC algorithm on a manned aircraft that can achieve a high level of dynamic similarity to a launch vehicle and raise the technology readiness of the algorithm early in the program. This document reports the outcome of the NESC assessment.

  6. Development of an Antimicrobial Susceptibility Testing Method Suitable for Performing During Space Flight

    Science.gov (United States)

    Jorgensen, James H.; Skweres, Joyce A.; Mishra S. K.; McElmeel, M. Letticia; Maher, Louise A.; Mulder, Ross; Lancaster, Michael V.; Pierson, Duane L.

    1997-01-01

    Very little is known regarding the affects of the microgravity environment of space flight upon the action of antimicrobial agents on bacterial pathogens. This study was undertaken to develop a simple method for conducting antibacterial susceptibility tests during a Space Shuttle mission. Specially prepared susceptibility test research cards (bioMerieux Vitek, Hazelwood, MO) were designed to include 6-11 serial two-fold dilutions of 14 antimicrobial agents, including penicillins, cephalosporins, a Beta-lactamase inhibitor, vancomycin, erythromycin, tetracycline, gentamicin, ciprofloxacin, and trimethoprim/sulfamethoxazole. Minimal inhibitory concentrations (MICS) of the drugs were determined by visual reading of color endpoints in the Vitek research cards made possible by incorporation of a colorimetric growth indicator (alamarBlue(Trademark), Accumed International, Westlake, OH). This study has demonstrated reproducible susceptibility results when testing isolates of Staphylococcus aurezis, Group A Streptococcus, Enterococcusfaecalis, Escherichia coli (beta-lactamase positive and negative strains), Klebsiella pneumoniae, Enterobacter cloacae, and Pseudomoiias aeruginosa. In some instances, the MICs were comparable to those determined using a standard broth microdilution method, while in some cases the unique test media and format yielded slightly different values, that were themselves reproducible. The proposed in-flight experiment will include inoculation of the Vitek cards on the ground prior to launch of the Space Shuttle, storage of inoculated cards at refrigeration temperature aboard the Space Shuttle until experiment initiation, then incubation of the cards for 18-48 h prior to visual interpretation of MICs by the mission's astronauts. Ground-based studies have shown reproducible MICs following storage of inoculated cards for 7 days at 4-8 C to accommodate the mission's time schedule and the astronauts' activities. For comparison, ground-based control

  7. Flight Test Comparison of Different Adaptive Augmentations for Fault Tolerant Control Laws for a Modified F-15 Aircraft

    Science.gov (United States)

    Burken, John J.; Hanson, Curtis E.; Lee, James A.; Kaneshige, John T.

    2009-01-01

    This report describes the improvements and enhancements to a neural network based approach for directly adapting to aerodynamic changes resulting from damage or failures. This research is a follow-on effort to flight tests performed on the NASA F-15 aircraft as part of the Intelligent Flight Control System research effort. Previous flight test results demonstrated the potential for performance improvement under destabilizing damage conditions. Little or no improvement was provided under simulated control surface failures, however, and the adaptive system was prone to pilot-induced oscillations. An improved controller was designed to reduce the occurrence of pilot-induced oscillations and increase robustness to failures in general. This report presents an analysis of the neural networks used in the previous flight test, the improved adaptive controller, and the baseline case with no adaptation. Flight test results demonstrate significant improvement in performance by using the new adaptive controller compared with the previous adaptive system and the baseline system for control surface failures.

  8. Ground testing and flight demonstration of charge management of insulated test masses using UV-LED electron photoemission

    Science.gov (United States)

    Saraf, Shailendhar; Buchman, Sasha; Balakrishnan, Karthik; Lui, Chin Yang; Soulage, Michael; Faied, Dohy; Hanson, John; Ling, Kuok; Jaroux, Belgacem; Suwaidan, Badr Al; AlRashed, Abdullah; Al-Nassban, Badr; Alaqeel, Faisal; Harbi, Mohammed Al; Salamah, Badr Bin; Othman, Mohammed Bin; Qasim, Bandar Bin; Alfauwaz, Abdulrahman; Al-Majed, Mohammed; DeBra, Daniel; Byer, Robert

    2016-12-01

    The UV-LED mission demonstrates the precise control of the potential of electrically isolated test masses. Test mass charge control is essential for the operation of space accelerometers and drag-free sensors which are at the core of geodesy, aeronomy and precision navigation missions as well as gravitational wave experiments and observatories. Charge management using photoelectrons generated by the 254 nm UV line of Hg was first demonstrated on Gravity Probe B and is presently part of the LISA Pathfinder technology demonstration. The UV-LED mission and prior ground testing demonstrates that AlGaN UVLEDs operating at 255 nm are superior to Hg lamps because of their smaller size, lower power draw, higher dynamic range, and higher control authority. We show laboratory data demonstrating the effectiveness and survivability of the UV-LED devices and performance of the charge management system. We also show flight data from a small satellite experiment that was one of the payloads on KACST’s SaudiSat-4 mission that demonstrates ‘AC charge control’ (UV-LEDs and bias are AC modulated with adjustable relative phase) between a spherical test mass and its housing. The result of the mission brings the UV-LED device Technology Readiness Level (TRL) to TRL-9 and the charge management system to TRL-7. We demonstrate the ability to control the test mass potential on an 89 mm diameter spherical test mass over a 20 mm gap in a drag-free system configuration, with potential measured using an ultra-high impedance contact probe. Finally, the key electrical and optical characteristics of the UV-LEDs showed less than 7.5% change in performance after 12 months in orbit.

  9. Ground testing and flight demonstration of charge management of insulated test masses using UV-LED electron photoemission

    International Nuclear Information System (INIS)

    Saraf, Shailendhar; Buchman, Sasha; Balakrishnan, Karthik; Lui, Chin Yang; Alfauwaz, Abdulrahman; DeBra, Daniel; Soulage, Michael; Faied, Dohy; Hanson, John; Ling, Kuok; Jaroux, Belgacem; Suwaidan, Badr Al; AlRashed, Abdullah; Al-Nassban, Badr; Alaqeel, Faisal; Harbi, Mohammed Al; Salamah, Badr Bin; Othman, Mohammed Bin; Qasim, Bandar Bin; Al-Majed, Mohammed

    2016-01-01

    The UV-LED mission demonstrates the precise control of the potential of electrically isolated test masses. Test mass charge control is essential for the operation of space accelerometers and drag-free sensors which are at the core of geodesy, aeronomy and precision navigation missions as well as gravitational wave experiments and observatories. Charge management using photoelectrons generated by the 254 nm UV line of Hg was first demonstrated on Gravity Probe B and is presently part of the LISA Pathfinder technology demonstration. The UV-LED mission and prior ground testing demonstrates that AlGaN UVLEDs operating at 255 nm are superior to Hg lamps because of their smaller size, lower power draw, higher dynamic range, and higher control authority. We show laboratory data demonstrating the effectiveness and survivability of the UV-LED devices and performance of the charge management system. We also show flight data from a small satellite experiment that was one of the payloads on KACST’s SaudiSat-4 mission that demonstrates ‘AC charge control’ (UV-LEDs and bias are AC modulated with adjustable relative phase) between a spherical test mass and its housing. The result of the mission brings the UV-LED device Technology Readiness Level (TRL) to TRL-9 and the charge management system to TRL-7. We demonstrate the ability to control the test mass potential on an 89 mm diameter spherical test mass over a 20 mm gap in a drag-free system configuration, with potential measured using an ultra-high impedance contact probe. Finally, the key electrical and optical characteristics of the UV-LEDs showed less than 7.5% change in performance after 12 months in orbit. (paper)

  10. The Time-Of-Flight detector of ALICE at LHC: construction, test and commissioning with cosmic rays

    CERN Document Server

    Preghenella, Roberto

    2009-01-01

    After several years of research and development the Time-Of-Flight detector of ALICE (A Large Ion Collider Experiment) has been constructed and is presently fully installed and operative in the experimental area located at the interaction point n.2 of the LHC (Large Hadron Collider) at CERN. Particle identification in ALICE is essential, as many observables are either mass or flavour dependent, therefore many different techniques are used to cover the largest possible momentum range. As said, the TOF (Time- Of-Flight) detector, of which a comprehensive review is given in Chapter 2, is dedicated to hadron identification at medium momenta. The detector exploits the novel technology based on the Multigap Resistive Plate Chamber (MRPC) which guarantees the excellent performance required for a very large time-of-flight array. The construction of the ALICE TOF detector has required the assembly of a large number of MRPC detectors which has been successfully carried out thanks to a careful mass production controlled...

  11. COBALT: Development of a Platform to Flight Test Lander GN&C Technologies on Suborbital Rockets

    Science.gov (United States)

    Carson, John M., III; Seubert, Carl R.; Amzajerdian, Farzin; Bergh, Chuck; Kourchians, Ara; Restrepo, Carolina I.; Villapando, Carlos Y.; O'Neal, Travis V.; Robertson, Edward A.; Pierrottet, Diego; hide

    2017-01-01

    The NASA COBALT Project (CoOperative Blending of Autonomous Landing Technologies) is developing and integrating new precision-landing Guidance, Navigation and Control (GN&C) technologies, along with developing a terrestrial fight-test platform for Technology Readiness Level (TRL) maturation. The current technologies include a third- generation Navigation Doppler Lidar (NDL) sensor for ultra-precise velocity and line- of-site (LOS) range measurements, and the Lander Vision System (LVS) that provides passive-optical Terrain Relative Navigation (TRN) estimates of map-relative position. The COBALT platform is self contained and includes the NDL and LVS sensors, blending filter, a custom compute element, power unit, and communication system. The platform incorporates a structural frame that has been designed to integrate with the payload frame onboard the new Masten Xodiac vertical take-o, vertical landing (VTVL) terrestrial rocket vehicle. Ground integration and testing is underway, and terrestrial fight testing onboard Xodiac is planned for 2017 with two flight campaigns: one open-loop and one closed-loop.

  12. Five years of testing using the simi-automated ultrasonic time of flight diffraction system

    International Nuclear Information System (INIS)

    Webber, S.A.

    2002-01-01

    This paper provides a brief description of the Time of Flight Diffraction (TOFD) test system and also describes a couple of case histories where the system has been successfully applied. The T.O.F.D. system has been contrasted with the conventional manual ultrasonic technique. Whilst the T.O.F.D. system has proven potential, and is without doubt a valuable tool that will continue to gain market share in the inspection industry, conventional manual ultrasonics still has its part to play and will survive for some time to come. One of the outstanding issues facing the T.O.F.D. systems is the question of acceptance testing which is still the predominant convention specified in most standards. Training for a T.O.F.D. system technician is particularly important and the author suggests there are more traps for the unwary than with the conventional manual ultrasonic systems. The overall judgement of the T.O.F.D. system is that it is a most welcome and powerful tool in the hands of the right operator and will do much to boost the prominence of Non-Destructive Testing

  13. Direct analysis and identification of pathogenic Lichtheimia species by matrix-assisted laser desorption ionization-time of flight analyzer-mediated mass spectrometry.

    Science.gov (United States)

    Schrödl, Wieland; Heydel, Tilo; Schwartze, Volker U; Hoffmann, Kerstin; Grosse-Herrenthey, Anke; Walther, Grit; Alastruey-Izquierdo, Ana; Rodriguez-Tudela, Juan Luis; Olias, Philipp; Jacobsen, Ilse D; de Hoog, G Sybren; Voigt, Kerstin

    2012-02-01

    Zygomycetes of the order Mucorales can cause life-threatening infections in humans. These mucormycoses are emerging and associated with a rapid tissue destruction and high mortality. The resistance of Mucorales to antimycotic substances varies between and within clinically important genera such as Mucor, Rhizopus, and Lichtheimia. Thus, an accurate diagnosis before onset of antimycotic therapy is recommended. Matrix-assisted laser desorption ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) is a potentially powerful tool to rapidly identify infectious agents on the species level. We investigated the potential of MALDI-TOF MS to differentiate Lichtheimia species, one of the most important agents of mucormycoses. Using the Bruker Daltonics FlexAnalysis (version 3.0) software package, a spectral database library with m/z ratios of 2,000 to 20,000 Da was created for 19 type and reference strains of clinically relevant Zygomycetes of the order Mucorales (12 species in 7 genera). The database was tested for accuracy by use of 34 clinical and environmental isolates of Lichtheimia comprising a total of five species. Our data demonstrate that MALDI-TOF MS can be used to clearly discriminate Lichtheimia species from other pathogenic species of the Mucorales. Furthermore, the method is suitable to discriminate species within the genus. The reliability and robustness of the MALDI-TOF-based identification are evidenced by high score values (above 2.3) for the designation to a certain species and by moderate score values (below 2.0) for the discrimination between clinically relevant (Lichtheimia corymbifera, L. ramosa, and L. ornata) and irrelevant (L. hyalospora and L. sphaerocystis) species. In total, all 34 strains were unequivocally identified by MALDI-TOF MS with score values of >1.8 down to the generic level, 32 out of 34 of the Lichtheimia isolates (except CNM-CM 5399 and FSU 10566) were identified accurately with score values of >2 (probable species

  14. Direct Analysis and Identification of Pathogenic Lichtheimia Species by Matrix-Assisted Laser Desorption Ionization–Time of Flight Analyzer-Mediated Mass Spectrometry

    Science.gov (United States)

    Schrödl, Wieland; Heydel, Tilo; Schwartze, Volker U.; Hoffmann, Kerstin; Große-Herrenthey, Anke; Walther, Grit; Alastruey-Izquierdo, Ana; Rodriguez-Tudela, Juan Luis; Olias, Philipp; Jacobsen, Ilse D.; de Hoog, G. Sybren

    2012-01-01

    Zygomycetes of the order Mucorales can cause life-threatening infections in humans. These mucormycoses are emerging and associated with a rapid tissue destruction and high mortality. The resistance of Mucorales to antimycotic substances varies between and within clinically important genera such as Mucor, Rhizopus, and Lichtheimia. Thus, an accurate diagnosis before onset of antimycotic therapy is recommended. Matrix-assisted laser desorption ionization (MALDI)–time of flight (TOF) mass spectrometry (MS) is a potentially powerful tool to rapidly identify infectious agents on the species level. We investigated the potential of MALDI-TOF MS to differentiate Lichtheimia species, one of the most important agents of mucormycoses. Using the Bruker Daltonics FlexAnalysis (version 3.0) software package, a spectral database library with m/z ratios of 2,000 to 20,000 Da was created for 19 type and reference strains of clinically relevant Zygomycetes of the order Mucorales (12 species in 7 genera). The database was tested for accuracy by use of 34 clinical and environmental isolates of Lichtheimia comprising a total of five species. Our data demonstrate that MALDI-TOF MS can be used to clearly discriminate Lichtheimia species from other pathogenic species of the Mucorales. Furthermore, the method is suitable to discriminate species within the genus. The reliability and robustness of the MALDI-TOF-based identification are evidenced by high score values (above 2.3) for the designation to a certain species and by moderate score values (below 2.0) for the discrimination between clinically relevant (Lichtheimia corymbifera, L. ramosa, and L. ornata) and irrelevant (L. hyalospora and L. sphaerocystis) species. In total, all 34 strains were unequivocally identified by MALDI-TOF MS with score values of >1.8 down to the generic level, 32 out of 34 of the Lichtheimia isolates (except CNM-CM 5399 and FSU 10566) were identified accurately with score values of >2 (probable species

  15. Structural elucidation and identification of a new derivative of phenethylamine using quadrupole time-of-flight mass spectrometry.

    Science.gov (United States)

    Sekuła, Karolina; Zuba, Dariusz

    2013-09-30

    In recent years, the phenomenon of uncontrolled distribution of new psychoactive substances that were marketed without prior toxicological studies has been observed. Because many designer drugs are related in chemical structure, the potential for misidentifying them is an important problem. It is therefore essential to develop an analytical procedure for unequivocal elucidation of the structures of these compounds. The issue has been discussed in the context of 25I-NBMD [2-(4-iodo-2,5-dimethoxyphenyl)-N-[(2,3-methylenedioxyphenyl)methyl]ethanamine], a psychoactive substance first discovered on the drug market in 2012. The substance was extracted from blotter papers with methanol. Separation was achieved via liquid chromatography. Analysis was conducted by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QTOFMS). Identification of the psychoactive component was supported by electron impact gas chromatography/mass spectrometry (GC/EI-MS). The high accuracy of the LC/ESI-QTOFMS method allowed the molecular mass of the investigated substance (M(exp) = 441.0438 Da; mass error, ∆m = 0.2 ppm) and the formulae of ions formed during fragmentation to be determined. The main ions were recorded at m/z = 135.0440, 290.9876 and 305.9981. Structures of the obtained ions were elucidated in the tandem mass spectrometry (MS/MS) experiments by comparing them to mass spectra of previously detected derivatives of phenethylamine. The performed study indicated the potential for using LC/QTOFMS method to identify new designer drugs. This technique can be used supplementary to standard GC/MS. Prior knowledge of the fragmentation mechanisms of phenethylamines allowed to predict the mass spectra of the novel substance--25I-NBMD. Copyright © 2013 John Wiley & Sons, Ltd.

  16. Identification of differential metabolites in liquid diet fermented with Bacillus subtilis using gas chromatography time of flight mass spectrometry

    Directory of Open Access Journals (Sweden)

    Yuyong He

    2016-12-01

    Full Text Available Growth and health responses of pigs fed fermented liquid diet are not always consistent and causes for this issue are still not very clear. Metabolites produced at different fermentation time points should be one of the most important contributors. However, currently no literatures about differential metabolites of fermented liquid diet are reported. The aim of this experiment was to explore the difference of metabolites in a fermented liquid diet between different fermentation time intervals. A total of eighteen samples that collected from Bacillus subtilis fermented liquid diet on days 7, 21 and 35 respectively were used for the identification of metabolites by gas chromatography time of flight mass spectrometry (GC-TOF-MS. Fifteen differential metabolites including melibiose, sortitol, ribose, cellobiose, maltotriose, sorbose, isomaltose, maltose, fructose, d-glycerol-1-phosphate, 4-aminobutyric acid, beta-alanine, tyrosine, pyruvic acid and pantothenic acid were identified between 7-d samples and 21-d samples. The relative level of melibiose, ribose, maltotriose, d-glycerol-1-phosphate, tyrosine and pyruvic acid in samples collected on day 21 was significantly higher than that in samples collected on day 7 (P < 0.01, respectively. Eight differential metabolites including ribose, sorbose, galactinol, cellobiose, pyruvic acid, galactonic acid, pantothenic acid and guanosine were found between 21-d samples and 35-d samples. Samples collected on day 35 had a higher relative level of ribose than that in samples collected on day 21 (P < 0.01. In conclusion, many differential metabolites which have important effects on the growth and health of pigs are identified and findings contribute to explain the difference in feeding response of fermented liquid diet.

  17. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: a new possibility for the identification and typing of anaerobic bacteria.

    Science.gov (United States)

    Nagy, Elizabeth

    2014-01-01

    Anaerobic bacteria predominate in the normal flora of humans and are important, often life-threatening pathogens in mixed infections originating from the indigenous microbiota. The isolation and identification of anaerobes by phenotypic and DNA-based molecular methods at a species level is time-consuming and laborious. Following the successful adaptation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the routine laboratory identification of bacteria, the extensive development of a database has been initiated to use this method for the identification of anaerobic bacteria. Not only frequently isolated anaerobic species, but also newly recognized and taxonomically rearranged genera and species can be identified using direct smear samples or whole-cell protein extraction, and even phylogenetically closely related species can be identified correctly by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Typing of anaerobic bacteria on a subspecies level, determination of antibiotic resistance and direct identification of blood culture isolates will revolutionize anaerobe bacteriology in the near future.

  18. Vibratory gyroscopes : identification of mathematical model from test data

    CSIR Research Space (South Africa)

    Shatalov, MY

    2007-05-01

    Full Text Available Simple mathematical model of vibratory gyroscopes imperfections is formulated, which includes anisotropic damping and variation of mass-stiffness parameters and their harmonics. The method of identification of parameters of the mathematical model...

  19. Multicenter Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Gram-Positive Aerobic Bacteria

    Science.gov (United States)

    Burnham, Carey-Ann D.; Bythrow, Maureen; Garner, Omai B.; Ginocchio, Christine C.; Jennemann, Rebecca; Lewinski, Michael A.; Manji, Ryhana; Mochon, A. Brian; Procop, Gary W.; Richter, Sandra S.; Sercia, Linda; Westblade, Lars F.; Ferraro, Mary Jane; Branda, John A.

    2013-01-01

    Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF) is gaining momentum as a tool for bacterial identification in the clinical microbiology laboratory. Compared with conventional methods, this technology can more readily and conveniently identify a wide range of organisms. Here, we report the findings from a multicenter study to evaluate the Vitek MS v2.0 system (bioMérieux, Inc.) for the identification of aerobic Gram-positive bacteria. A total of 1,146 unique isolates, representing 13 genera and 42 species, were analyzed, and results were compared to those obtained by nucleic acid sequence-based identification as the reference method. For 1,063 of 1,146 isolates (92.8%), the Vitek MS provided a single identification that was accurate to the species level. For an additional 31 isolates (2.7%), multiple possible identifications were provided, all correct at the genus level. Mixed-genus or single-choice incorrect identifications were provided for 18 isolates (1.6%). Although no identification was obtained for 33 isolates (2.9%), there was no specific bacterial species for which the Vitek MS consistently failed to provide identification. In a subset of 463 isolates representing commonly encountered important pathogens, 95% were accurately identified to the species level and there were no misidentifications. Also, in all but one instance, the Vitek MS correctly differentiated Streptococcus pneumoniae from other viridans group streptococci. The findings demonstrate that the Vitek MS system is highly accurate for the identification of Gram-positive aerobic bacteria in the clinical laboratory setting. PMID:23658261

  20. Multicenter evaluation of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of Gram-positive aerobic bacteria.

    Science.gov (United States)

    Rychert, Jenna; Burnham, Carey-Ann D; Bythrow, Maureen; Garner, Omai B; Ginocchio, Christine C; Jennemann, Rebecca; Lewinski, Michael A; Manji, Ryhana; Mochon, A Brian; Procop, Gary W; Richter, Sandra S; Sercia, Linda; Westblade, Lars F; Ferraro, Mary Jane; Branda, John A

    2013-07-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) is gaining momentum as a tool for bacterial identification in the clinical microbiology laboratory. Compared with conventional methods, this technology can more readily and conveniently identify a wide range of organisms. Here, we report the findings from a multicenter study to evaluate the Vitek MS v2.0 system (bioMérieux, Inc.) for the identification of aerobic Gram-positive bacteria. A total of 1,146 unique isolates, representing 13 genera and 42 species, were analyzed, and results were compared to those obtained by nucleic acid sequence-based identification as the reference method. For 1,063 of 1,146 isolates (92.8%), the Vitek MS provided a single identification that was accurate to the species level. For an additional 31 isolates (2.7%), multiple possible identifications were provided, all correct at the genus level. Mixed-genus or single-choice incorrect identifications were provided for 18 isolates (1.6%). Although no identification was obtained for 33 isolates (2.9%), there was no specific bacterial species for which the Vitek MS consistently failed to provide identification. In a subset of 463 isolates representing commonly encountered important pathogens, 95% were accurately identified to the species level and there were no misidentifications. Also, in all but one instance, the Vitek MS correctly differentiated Streptococcus pneumoniae from other viridans group streptococci. The findings demonstrate that the Vitek MS system is highly accurate for the identification of Gram-positive aerobic bacteria in the clinical laboratory setting.

  1. Direct identification of pathogens from positive blood cultures using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Rodríguez-Sánchez, B; Sánchez-Carrillo, C; Ruiz, A; Marín, M; Cercenado, E; Rodríguez-Créixems, M; Bouza, E

    2014-07-01

    In recent years, matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has proved a rapid and reliable method for the identification of bacteria and yeasts that have already been isolated. The objective of this study was to evaluate this technology as a routine method for the identification of microorganisms directly from blood culture bottles (BCBs), before isolation, in a large collection of samples. For this purpose, 1000 positive BCBs containing 1085 microorganisms have been analysed by conventional phenotypic methods and by MALDI-TOF MS. Discrepancies have been resolved using molecular methods: the amplification and sequencing of the 16S rRNA gene or the Superoxide Dismutase gene (sodA) for streptococcal isolates. MALDI-TOF predicted a species- or genus-level identification of 81.4% of the analysed microorganisms. The analysis by episode yielded a complete identification of 814 out of 1000 analysed episodes (81.4%). MALDI-TOF identification is available for clinicians within hours of a working shift, as oppose to 18 h later when conventional identification methods are performed. Moreover, although further improvement of sample preparation for polymicrobial BCBs is required, the identification of more than one pathogen in the same BCB provides a valuable indication of unexpected pathogens when their presence may remain undetected in Gram staining. Implementation of MALDI-TOF identification directly from the BCB provides a rapid and reliable identification of the causal pathogen within hours. © 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases.

  2. Manned/Unmanned Common Architecture Program (MCAP) net centric flight tests

    Science.gov (United States)

    Johnson, Dale

    2009-04-01

    Properly architected avionics systems can reduce the costs of periodic functional improvements, maintenance, and obsolescence. With this in mind, the U.S. Army Aviation Applied Technology Directorate (AATD) initiated the Manned/Unmanned Common Architecture Program (MCAP) in 2003 to develop an affordable, high-performance embedded mission processing architecture for potential application to multiple aviation platforms. MCAP analyzed Army helicopter and unmanned air vehicle (UAV) missions, identified supporting subsystems, surveyed advanced hardware and software technologies, and defined computational infrastructure technical requirements. The project selected a set of modular open systems standards and market-driven commercial-off-theshelf (COTS) electronics and software, and, developed experimental mission processors, network architectures, and software infrastructures supporting the integration of new capabilities, interoperability, and life cycle cost reductions. MCAP integrated the new mission processing architecture into an AH-64D Apache Longbow and participated in Future Combat Systems (FCS) network-centric operations field experiments in 2006 and 2007 at White Sands Missile Range (WSMR), New Mexico and at the Nevada Test and Training Range (NTTR) in 2008. The MCAP Apache also participated in PM C4ISR On-the-Move (OTM) Capstone Experiments 2007 (E07) and 2008 (E08) at Ft. Dix, NJ and conducted Mesa, Arizona local area flight tests in December 2005, February 2006, and June 2008.

  3. Measurement of a Peak in the Cosmic Microwave Background Power Spectrum from the North American test flight of BOOMERANG

    CERN Document Server

    Mauskopf, P D; De Bernardis, P; Bock, J J; Borrill, J; Boscaleri, A; Crill, B P; De Gasperis, G; De Troia, G; Farese, P; Ferreira, P G; Ganga, K; Giacometti, M; Hanany, S; Hristov, V V; Iacoangeli, A; Jaffe, A H; Lange, A E; Lee, A T; Masi, S; Melchiorri, A; Melchiorri, F; Miglio, L; Montroy, T; Netterfield, C B; Pascale, E; Piacentini, F; Richards, P L; Romeo, G; Ruhl, J E; Scannapieco, E S; Scaramuzzi, F; Stompor, R; Vittorio, N

    2000-01-01

    We describe a measurement of the angular power spectrum of anisotropies in the Cosmic Microwave Background (CMB) from 0.3 degrees to ~10 degrees from the North American test flight of the BOOMERANG experiment. BOOMERANG is a balloon-borne telescope with a bolometric receiver designed to map CMB anisotropies on a Long Duration Balloon flight. During a 6-hour test flight of a prototype system in 1997, we mapped > 200 square degrees at high galactic latitudes in two bands centered at 90 and 150 GHz with a resolution of 26 and 16.6 arcmin FWHM respectively. Analysis of the maps gives a power spectrum with a peak at angular scales of ~1 degree with an amplitude ~70 uK.

  4. Measurement of a peak in the cosmic microwave background power spectrum from the North American test flight of Boomerang

    International Nuclear Information System (INIS)

    Mauskopf, P.D.; Ade, P.A.R.; Bock, J.J.; Borrill, J.; Boscaleri, A.; Crill, B.P.; Bernardis, P. de; DeGasperis, G.; De Troia, G.; Farese, P.; Ferreira, P.G.; Ganga, K.; Giacometti, M.; Hanany, S.; Hristov, V.V.; Iacoangeli, A.; Jaffe, A.H.; Lange, A.E.; Lee, A.T.; Masi, S.; Melchiorri, A.; Melchiorri, F.; Miglio, L.; Montroy, T.; Netterfield, C.B.; Pascale, E.; Piacentini, F.; Richards, P.L.; Romeo, G.; Ruhl, J.E.; Scannapieco, E.; Scaramuzzi, F.; Stompor, R.; Vittorio, N.

    1999-01-01

    We describe a measurement of the angular power spectrum of anisotrophies in the Cosmic Microwave Background (CMB) from 0.2 deg to approx. 10 deg. from the test flight of the BOOMERANG experiment. BOOMERANG is a balloon-borne telescope with a bolometric receiver designed to map CMB anisotrophies on a Long Duration Balloon flight. During a 6-hour test flight of a prototype system in 1997, we mapped > 200 square degrees at high galactic latitudes in two bands centered at 90 and 150 GHz with a resolution of 26 and 16.6 arcmin FWHM respectively. Analysis of the maps gives a power spectrum with a peak at angular scales of approx. 1 deg. with an amplitude of approx. 70-muKcmb

  5. Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting.

    Science.gov (United States)

    Christner, Martin; Rohde, Holger; Wolters, Manuel; Sobottka, Ingo; Wegscheider, Karl; Aepfelbacher, Martin

    2010-05-01

    Early and adequate antimicrobial therapy has been shown to improve the clinical outcome in bloodstream infections (BSI). To provide rapid pathogen identification for targeted treatment, we applied matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry fingerprinting to bacteria directly recovered from blood culture bottles. A total of 304 aerobic and anaerobic blood cultures, reported positive by a Bactec 9240 system, were subjected in parallel to differential centrifugation with subsequent mass spectrometry fingerprinting and reference identification using established microbiological methods. A representative spectrum of bloodstream pathogens was recovered from 277 samples that grew a single bacterial isolate. Species identification by direct mass spectrometry fingerprinting matched reference identification in 95% of these samples and worked equally well for aerobic and anaerobic culture bottles. Application of commonly used score cutoffs to classify the fingerprinting results led to an identification rate of 87%. Mismatching mostly resulted from insufficient bacterial numbers and preferentially occurred with Gram-positive samples. The respective spectra showed low concordance to database references and were effectively rejected by score thresholds. Spiking experiments and examination of the respective study samples even suggested applicability of the method to mixed cultures. With turnaround times around 100 min, the approach allowed for reliable pathogen identification at the day of blood culture positivity, providing treatment-relevant information within the critical phase of septic illness.

  6. Evaluation of VITEK mass spectrometry (MS), a matrix-assisted laser desorption ionization time-of-flight MS system for identification of anaerobic bacteria.

    Science.gov (United States)

    Lee, Wonmok; Kim, Myungsook; Yong, Dongeun; Jeong, Seok Hoon; Lee, Kyungwon; Chong, Yunsop

    2015-01-01

    By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMérieux, France) in the identification of anaerobes. We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing. Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level. The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria.

  7. Reliability and validity of a talent identification test battery for seated and standing Paralympic throws.

    Science.gov (United States)

    Spathis, Jemima Grace; Connick, Mark James; Beckman, Emma Maree; Newcombe, Peter Anthony; Tweedy, Sean Michael

    2015-01-01

    Paralympic throwing events for athletes with physical impairments comprise seated and standing javelin, shot put, discus and seated club throwing. Identification of talented throwers would enable prediction of future success and promote participation; however, a valid and reliable talent identification battery for Paralympic throwing has not been reported. This study evaluates the reliability and validity of a talent identification battery for Paralympic throws. Participants were non-disabled so that impairment would not confound analyses, and results would provide an indication of normative performance. Twenty-eight non-disabled participants (13 M; 15 F) aged 23.6 years (±5.44) performed five kinematically distinct criterion throws (three seated, two standing) and nine talent identification tests (three anthropometric, six motor); 23 were tested a second time to evaluate test-retest reliability. Talent identification test-retest reliability was evaluated using Intra-class Correlation Coefficient (ICC) and Bland-Altman plots (Limits of Agreement). Spearman's correlation assessed strength of association between criterion throws and talent identification tests. Reliability was generally acceptable (mean ICC = 0.89), but two seated talent identification tests require more extensive familiarisation. Correlation strength (mean rs = 0.76) indicated that the talent identification tests can be used to validly identify individuals with competitively advantageous attributes for each of the five kinematically distinct throwing activities. Results facilitate further research in this understudied area.

  8. Hollow Fiber Flight Prototype Spacesuit Water Membrane Evaporator Design and Testing

    Science.gov (United States)

    Bue, Grant; Vogel, Matt; Makinen, Janice; Tsioulos, Gus

    2010-01-01

    The spacesuit water membrane evaporator (SWME) is being developed to perform thermal control for advanced spacesuits and to take advantage of recent advances in micropore membrane technology. This results in a robust heat-rejection device that is potentially less sensitive to contamination than is the sublimator. The Membrana Celgard X50-215 microporous hollow-fiber (HoFi) membrane was selected after recent extensive testing as the most suitable candidate among commercial alternatives for continued SWME prototype development. The current design was based on a previous design that grouped the fiber layers into stacks, which were separated by small spaces and packaged into a cylindrical shape. This was developed into a full-scale prototype consisting of 14,300 tube bundled into 30 stacks, each of which is formed into a chevron shape and separated by spacers and organized into three sectors of 10 nested stacks. The new design replaced metal components with plastic ones, and has a custom built flight like backpressure valve mounted on the side of the SWME housing to reduce backpressure when fully open. The spacers that provided separation of the chevron fiber stacks were eliminated. Vacuum chamber testing showed improved heat rejection as a function of inlet water temperature and water vapor backpressure compared with the previous design. Other tests pushed the limits of tolerance to freezing and showed suitability to reject heat in a Mars pressure environment with and without a sweep gas. Tolerance to contamination by constituents expected to be found in potable water produced by distillation processes was tested in a conventional way by allowing constituents to accumulate in the coolant as evaporation occurs. For this purpose, the SWME cartridge has endured an equivalent of 30 EVAs exposure and demonstrated minimal performance decline.

  9. Hollow Fiber Space Water Membrane Evaporator Flight Prototype Design and Testing

    Science.gov (United States)

    Bue, Grant C.; Makinen, Janice; Vogel, Mtthew; Honas, Matt; Dillon, Paul; Colunga, Aaron; Truong, Lily; Porwitz, Darwin; Tsioulos, Gus

    2011-01-01

    The spacesuit water membrane evaporator (SWME) is being developed to perform thermal control for advanced spacesuits and to take advantage of recent advances in micropore membrane technology. This results in a robust heat-rejection device that is potentially less sensitive to contamination than is the sublimator. The current design was based on a previous design that grouped the fiber layers into stacks, which were separated by small spaces and packaged into a cylindrical shape. This was developed into a full-scale prototype consisting of 14,300 tube bundled into 30 stacks, each of which is formed into a chevron shape and separated by spacers and organized into three sectors of 10 nested stacks. The new design replaced metal components with plastic ones, eliminated the spacers, and has a custom built flight like backpressure valve mounted on the side of the SWME housing to reduce backpressure when fully open. A number of tests were performed in order to improve the strength of the polyurethane header that holds the fibers in place while the system is pressurized. Vacuum chamber testing showed similar heat rejection as a function of inlet water temperature and water vapor backpressure was similar to the previous design. Other tests pushed the limits of tolerance to freezing and showed suitability to reject heat in a Mars pressure environment with and without a sweep gas. Tolerance to contamination by constituents expected to be found in potable water produced by distillation processes was tested in a conventional way by allowing constituents to accumulate in the coolant as evaporation occurs. For this purpose, the SWME cartridge has endured an equivalent of 30 EVAs exposure and demonstrated acceptable performance decline.

  10. Validation test of advanced technology for IPV nickel-hydrogen flight cells: Update

    Science.gov (United States)

    Smithrick, John J.; Hall, Stephen W.

    1992-01-01

    Individual pressure vessel (IPV) nickel-hydrogen technology was advanced at NASA Lewis and under Lewis contracts with the intention of improving cycle life and performance. One advancement was to use 26 percent potassium hydroxide (KOH) electrolyte to improve cycle life. Another advancement was to modify the state-of-the-art cell design to eliminate identified failure modes. The modified design is referred to as the advanced design. A breakthrough in the low-earth-orbit (LEO) cycle life of IPV nickel-hydrogen cells has been previously reported. The cycle life of boiler plate cells containing 26 percent KOH electrolyte was about 40,000 LEO cycles compared to 3,500 cycles for cells containing 31 percent KOH. The boiler plate test results are in the process of being validated using flight hardware and real time LEO testing at the Naval Weapons Support Center (NWSC), Crane, Indiana under a NASA Lewis Contract. An advanced 125 Ah IPV nickel-hydrogen cell was designed. The primary function of the advanced cell is to store and deliver energy for long-term, LEO spacecraft missions. The new features of this design are: (1) use of 26 percent rather than 31 percent KOH electrolyte; (2) use of a patented catalyzed wall wick; (3) use of serrated-edge separators to facilitate gaseous oxygen and hydrogen flow within the cell, while still maintaining physical contact with the wall wick for electrolyte management; and (4) use of a floating rather than a fixed stack (state-of-the-art) to accommodate nickel electrode expansion due to charge/discharge cycling. The significant improvements resulting from these innovations are: extended cycle life; enhanced thermal, electrolyte, and oxygen management; and accommodation of nickel electrode expansion. The advanced cell design is in the process of being validated using real time LEO cycle life testing of NWSC, Crane, Indiana. An update of validation test results confirming this technology is presented.

  11. Results from an Interval Management (IM) Flight Test and Its Potential Benefit to Air Traffic Management Operations

    Science.gov (United States)

    Baxley, Brian; Swieringa, Kurt; Berckefeldt, Rick; Boyle, Dan

    2017-01-01

    NASA's first Air Traffic Management Technology Demonstration (ATD-1) subproject successfully completed a 19-day flight test of an Interval Management (IM) avionics prototype. The prototype was built based on IM standards, integrated into two test aircraft, and then flown in real-world conditions to determine if the goals of improving aircraft efficiency and airport throughput during high-density arrival operations could be met. The ATD-1 concept of operation integrates advanced arrival scheduling, controller decision support tools, and the IM avionics to enable multiple time-based arrival streams into a high-density terminal airspace. IM contributes by calculating airspeeds that enable an aircraft to achieve a spacing interval behind the preceding aircraft. The IM avionics uses its data (route of flight, position, etc.) and Automatic Dependent Surveillance-Broadcast (ADS-B) state data from the Target aircraft to calculate this airspeed. The flight test demonstrated that the IM avionics prototype met the spacing accuracy design goal for three of the four IM operation types tested. The primary issue requiring attention for future IM work is the high rate of IM speed commands and speed reversals. In total, during this flight test, the IM avionics prototype showed significant promise in contributing to the goals of improving aircraft efficiency and airport throughput.

  12. Testing of DNA isolation for the identification of hemp

    Directory of Open Access Journals (Sweden)

    Tomáš Vyhnánek

    2015-12-01

    Full Text Available Hemp is diploid organism (2n = 2x = 20, genome size 534 Mb with nine pairs of autosomes plus XX (♀ or XY (♂ chromosomes. Cannabis sativa L. is an important economic plant for the production of food, fibre, oils, and intoxicants. Genotypes (varieties or chemovar of hemp with low Δ9-tetrahydrocannabinol content are used for industrial applications. Varieties with high Δ9-tetrahydrocannabinol or high cannabidiol content are used for medicinal applications. Biochemical and molecular methods can be used for identification and classification. An important step for molecular biology methods is to obtain the matrix of the native and sufficiently pure DNA. We tested two different experimental variant of samples (20 mg and 100 mg of seeds, oilcake and dried flowers for analysis of the Italian variety Carmagnola for analysis (harvested in 2014, Hempoint Ltd., Czech Republic. The DNeasy® Plant Mini Kit (Qiagen, GE was used to isolate the DNA. The DNA concentration and purity was assessed by agarose electrophoresis and via a spectrophotometer. Samples of lower weight yielded lower values of DNA concentration (average 16.30 - 38.90 ng.µL-1, but with better purity than samples of higher weight (ratio A260nm/A280nm for low-weight samples was near 1.80. To test the applicability of DNA analysis, we used two SSR markers (CAN1347 and CAN2913. PCR products were separated on 1% agarose and on 8% polyacrylamide electrophoresis. DNA samples obtained from samples of higher weight exhibited less PCR amplification than samples of lower weight. We found no effect of sample weight on the formation of non-specific amplification products during the PCR reaction. Based on our results we can be recommended for practical isolation procedure using DNeasy® Plant Mini Kit with lower of sample weight (20 mg. In future work the procedure for DNA isolating from wheat-cannabis products, e. g. breads, rolls or pasta, will be optimized.

  13. Flight Testing of the Forward Osmosis Bag for Water Recovery on STS-135

    Science.gov (United States)

    Roberts, Michael S.; Soler, Monica; Mortenson, Todd; McCoy, LaShelle; Woodward, Spencer; Levine, Howard G.

    2011-01-01

    The Forward Osmosis Bag (FOB) is a personal water purification device for recovery of potable liquid from almost any non-potable water source. The FOB experiment was flown as a sortie mission on STS-135/ULF7 using flight-certified materials and a design based on the X-Pack(TradeMark) from Hydration Technology Innovations (Albany, OR). The primary objective was to validate the technology for use under microgravity conditions. The FOB utilizes a difference in solute concentration across a selectively permeable membrane to draw water molecules from the non-potable water while rejecting most chemical and all microbial contaminants contained within. Six FOB devices were tested on STS-135 for their ability to produce a potable liquid permeate from a feed solution containing 500 mL potassium chloride (15 g/L) amended with 0.1% methyl blue dye (w:v) tracer against an osmotic gradient created by addition of 60 mL of concentrate containing the osmolytes fructose and glucose, and 0.01% sodium fluorescein (w:v) tracer. Three FOB devices were physically mixed by hand for 2 minutes by a crewmember after loading to augment membrane wetting for comparison with three unmixed FOB devices. Hydraulic flux rate and rejection of salt and dye in microgravity were determined from a 60-mL sample collected by the crew on orbit after 6 hours. Post-flight analysis of samples collected on orbit demonstrated that the Forward Osmosis Bag achieved expected design specifications in microgravity. The hydraulic flux rate of water across the membrane was reduced approximately 50% in microgravity relative to ground controls that generated an average of 50 mL per hour using the same water and osmolyte solutions. The membrane rejected both potassium and chloride at >92% and methyl blue dye at >99.9%. Physical mixing of the FOB during water recovery did not have any significant effect on either flux rate or rejection of solutes from the water solution. The absence of buoyancy-driven convection in

  14. Comparison of two matrix-assisted laser desorption ionisation-time of flight mass spectrometry methods for the identification of clinically relevant anaerobic bacteria

    NARCIS (Netherlands)

    Veloo, A. C. M.; Knoester, M.; Degener, J. E.; Kuijper, E. J.

    2011-01-01

    Two commercially available MALDI-TOF MS systems, Bruker MS and Shimadzu MS, were compared for the identification of clinically relevant anaerobic bacteria. A selection of 79 clinical isolates, representing 19 different genera, were tested and compared with identification obtained by 16S rRNA gene

  15. Flight Test Techniques. Proceedings of the Flight Mechanics Panel Symposium Held in Lisbon, Portugal on 2-5 April 1984

    Science.gov (United States)

    1984-07-01

    E103 3000 200 270090 1800 Fig. 7: Antenna Radiation Pattern Aircraft: D028. Type of Antenna: Monopole Frequency: 1.5 6Nz, Bank Angle: 0±Z Deg. Ref...Pattern: - - - Monopole Mr 70 250 F 4g. 8: S.uae1tpcl S0OA0r a) Test Emitter: Frequency Range UHF -18 GHz Antenna Parabolic Dish Wln Horn Feeds Transmitter...essais d’extinction et rallumages moteur, de flottement, etc.. Un effort sp~cial a 6t consacr6, Ai Istres, A Ia mice en place de moyens et dispositifs

  16. Handling Qualities Flight Testing of the Stratospheric Observatory for Infrared Astronomy (SOFIA)

    Science.gov (United States)

    Glaser, Scott T.; Strovers, Brian K.

    2011-01-01

    Airborne infrared astronomy has a long successful history, albeit relatively unknown outside of the astronomy community. A major problem with ground based infrared astronomy is the absorption and scatter of infrared energy by water in the atmosphere. Observing the universe from above 40,000 ft puts the observation platform above 99% of the water vapor in the atmosphere, thereby addressing this problem at a fraction of the cost of space based systems. The Stratospheric Observatory For Infrared Astronomy (SOFIA) aircraft is the most ambitious foray into the field of airborne infrared astronomy in history. Using a 747SP (The Boeing Company, Chicago, Illinois) aircraft modified with a 2.5m telescope located in the aft section of the fuselage, the SOFIA endeavors to provide views of the universe never before possible and at a fraction of the cost of space based systems. The modification to the airplane includes moveable doors and aperture that expose the telescope assembly. The telescope assembly is aimed and stabilized using a multitude of on board systems. This modification has the potential to cause aerodynamic anomalies that could induce undesired forces either at the cavity itself or indirectly due to interference with the empennage, both of which could cause handling qualities issues. As a result, an extensive analysis and flight test program was conducted from December 2009 through March 2011. Several methods, including a Lower Order Equivalent Systems analysis and pilot assessment, were used to ascertain the effects of the modification. The SOFIA modification was found to cause no adverse handling qualities effects and the aircraft was cleared for operational use. This paper discusses the history and modification to the aircraft, development of test procedures and analysis, results of testing and analysis, lessons learned for future projects and justification for operational certification.

  17. The development of a SAR dedicated navigation system: from scratch to the first test flight: 2004BU1-RE

    NARCIS (Netherlands)

    Lorga, J.F.M.; Rossum, W.L. van; Halsema, D. van; Chu, Q.P.; Mulder, J.A.

    2004-01-01

    In this paper, the authors propose to describe the development process of a navigation system, concerning Syntectic Aperture Radar (SAR) applications, starting from the motivation for the sensor selection and finalizing with the first flight-test results. Sensors selection was one of the first steps

  18. Identification of rare pathogenic bacteria in a clinical microbiology laboratory: impact of matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    Science.gov (United States)

    Seng, Piseth; Abat, Cedric; Rolain, Jean Marc; Colson, Philippe; Lagier, Jean-Christophe; Gouriet, Frédérique; Fournier, Pierre Edouard; Drancourt, Michel; La Scola, Bernard; Raoult, Didier

    2013-07-01

    During the past 5 years, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a powerful tool for routine identification in many clinical laboratories. We analyzed our 11-year experience in routine identification of clinical isolates (40 months using MALDI-TOF MS and 91 months using conventional phenotypic identification [CPI]). Among the 286,842 clonal isolates, 284,899 isolates of 459 species were identified. The remaining 1,951 isolates were misidentified and required confirmation using a second phenotypic identification for 670 isolates and using a molecular technique for 1,273 isolates of 339 species. MALDI-TOF MS annually identified 112 species, i.e., 36 species/10,000 isolates, compared to 44 species, i.e., 19 species/10,000 isolates, for CPI. Only 50 isolates required second phenotypic identifications during the MALDI-TOF MS period (i.e., 4.5 reidentifications/10,000 isolates) compared with 620 isolates during the CPI period (i.e., 35.2/10,000 isolates). We identified 128 bacterial species rarely reported as human pathogens, including 48 using phenotypic techniques (22 using CPI and 37 using MALDI-TOF MS). Another 75 rare species were identified using molecular methods. MALDI-TOF MS reduced the time required for identification by 55-fold and 169-fold and the cost by 5-fold and 96-fold compared with CPI and gene sequencing, respectively. MALDI-TOF MS was a powerful tool not only for routine bacterial identification but also for identification of rare bacterial species implicated in human infectious diseases. The ability to rapidly identify bacterial species rarely described as pathogens in specific clinical specimens will help us to study the clinical burden resulting from the emergence of these species as human pathogens, and MALDI-TOF MS may be considered an alternative to molecular methods in clinical laboratories.

  19. Direct identification of bacteria from charcoal-containing blood culture bottles using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.

    Science.gov (United States)

    Wüppenhorst, N; Consoir, C; Lörch, D; Schneider, C

    2012-10-01

    Several protocols for direct matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) from positive blood cultures are currently used to speed up the diagnostic process of bacteraemia. Identification rates are high and results are accurate for the BACTEC™ system and for charcoal-free bottles. Only a few studies have evaluated protocols for charcoal-containing BacT/ALERT bottles reaching substantially lower identification rates. We established a new protocol for sample preparation from aerobic and anaerobic positive charcoal-containing BacT/ALERT blood culture bottles and measured the protein profiles (n = 167). Then, we integrated this protocol in the routine workflow of our laboratory (n = 212). During the establishment of our protocol, 74.3 % of bacteria were correctly identified to the species level, in 23.4 %, no result and in 2.4 %, a false identification were obtained. Reliable criteria for correct species identification were a score value ≥1.400 and a best match on rank 1-3 of the same species. Identification rates during routine workflow were 77.8 % for correct identification, 20.8 % for not identified samples and 1.4 % for discordant identification. In conclusion, our results indicate that MALDI-TOF MS is possible, even from charcoal-containing blood cultures. Reliable criteria for correct species identification are a score value ≥1.400 and a best match on rank 1-3 of a single species.

  20. Comparison of Numerical Analyses with a Static Load Test of a Continuous Flight Auger Pile

    Science.gov (United States)

    Hoľko, Michal; Stacho, Jakub

    2014-12-01

    The article deals with numerical analyses of a Continuous Flight Auger (CFA) pile. The analyses include a comparison of calculated and measured load-settlement curves as well as a comparison of the load distribution over a pile's length. The numerical analyses were executed using two types of software, i.e., Ansys and Plaxis, which are based on FEM calculations. Both types of software are different from each other in the way they create numerical models, model the interface between the pile and soil, and use constitutive material models. The analyses have been prepared in the form of a parametric study, where the method of modelling the interface and the material models of the soil are compared and analysed. Our analyses show that both types of software permit the modelling of pile foundations. The Plaxis software uses advanced material models as well as the modelling of the impact of groundwater or overconsolidation. The load-settlement curve calculated using Plaxis is equal to the results of a static load test with a more than 95 % degree of accuracy. In comparison, the load-settlement curve calculated using Ansys allows for the obtaining of only an approximate estimate, but the software allows for the common modelling of large structure systems together with a foundation system.

  1. Reaction-in-flight neutrons as a test of stopping power in degenerate plasmas

    Science.gov (United States)

    Hayes, A. C.; Jungman, Gerard; Schulz, A. E.; Boswell, M.; Fowler, M. M.; Grim, G.; Klein, A.; Rundberg, R. S.; Wilhelmy, J. B.; Wilson, D.; Cerjan, C.; Schneider, D.; Sepke, S. M.; Tonchev, A.; Yeamans, C.

    2015-08-01

    We present the first measurements of reaction-in-flight (RIF) neutrons in an inertial confinement fusion system. The experiments were carried out at the National Ignition Facility, using both Low Foot and High Foot drives and cryogenic plastic capsules. In both cases, the high-energy RIF ( En> 15 MeV) component of the neutron spectrum was found to be about 10-4 of the total. The majority of the RIF neutrons were produced in the dense cold fuel surrounding the burning hotspot of the capsule, and the data are consistent with a compressed cold fuel that is moderately to strongly coupled (Γ˜ 0.6) and electron degenerate (θFermi/θe˜ 4). The production of RIF neutrons is controlled by the stopping power in the plasma. Thus, the current RIF measurements provide a unique test of stopping power models in an experimentally unexplored plasma regime. We find that the measured RIF data strongly constrain stopping models in warm dense plasma conditions, and some models are ruled out by our analysis of these experiments.

  2. The Use of Dynamic Visual Acuity as a Functional Test of Gaze Stabilization Following Space Flight

    Science.gov (United States)

    Peters, B. T.; Mulavara, A. P.; Brady, R.; Miller, C. A.; Richards, J. T.; Warren, L. E.; Cohen, H. S.; Bloomberg, J. J.

    2006-01-01

    After prolonged exposure to a given gravitational environment the transition to another is accompanied by adaptations in the sensorimotor subsystems, including the vestibular system. Variation in the adaptation time course of these subsystems, and the functional redundancies that exist between them make it difficult to accurately assess the functional capacity and physical limitations of astro/cosmonauts using tests on individual subsystems. While isolated tests of subsystem performance may be the only means to address where interventions are required, direct measures of performance may be more suitable for assessing the operational consequences of incomplete adaptation to changes in the gravitational environment. A test of dynamic visual acuity (DVA) is currently being used in the JSC Neurosciences Laboratory as part of a series of measures to assess the efficacy of a countermeasure to mitigate postflight locomotor dysfunction. In the current protocol, subjects visual acuity is determined using Landolt ring optotypes presented sequentially on a computer display. Visual acuity assessments are made both while standing and while walking at 1.8 m/s on a motorized treadmill. The use of a psychophysical threshold detection algorithm reduces the required number of optotype presentations and the results can be presented immediately after the test. The difference between the walking and standing acuity measures provides a metric of the change in the subject s ability to maintain gaze fixation on the visual target while walking. This functional consequence is observable regardless of the underlying subsystem most responsible for the change. Data from 15 cosmo/astronauts have been collected following long-duration (approx. 6 months) stays in space using a visual target viewing distance of 4.0 meters. An investigation of the group mean shows a change in DVA soon after the flight that asymptotes back to baseline approximately one week following their return to earth. The

  3. Planning fuel-conservative descents in an airline environmental using a small programmable calculator: algorithm development and flight test results

    Energy Technology Data Exchange (ETDEWEB)

    Knox, C.E.; Vicroy, D.D.; Simmon, D.A.

    1985-05-01

    A simple, airborne, flight-management descent algorithm was developed and programmed into a small programmable calculator. The algorithm may be operated in either a time mode or speed mode. The time mode was designed to aid the pilot in planning and executing a fuel-conservative descent to arrive at a metering fix at a time designated by the air traffic control system. The speed model was designed for planning fuel-conservative descents when time is not a consideration. The descent path for both modes was calculated for a constant with considerations given for the descent Mach/airspeed schedule, gross weight, wind, wind gradient, and nonstandard temperature effects. Flight tests, using the algorithm on the programmable calculator, showed that the open-loop guidance could be useful to airline flight crews for planning and executing fuel-conservative descents.

  4. Combined Raman/LIBS spectrometer elegant breadboard: built and tested - and flight model spectrometer unit

    Science.gov (United States)

    Ahlers, B.; Hutchinson, I.; Ingley, R.

    2017-11-01

    A spectrometer for combined Raman and Laser Induced Breakdown Spectroscopy (LIBS) is amongst the different instruments that have been pre-selected for the Pasteur payload of the ExoMars rover. It is regarded as a fundamental, next-generation instrument for organic, mineralogical and elemental characterisation of Martian soil, rock samples and organic molecules. Raman spectroscopy and LIBS will be integrated into a single instrument sharing many hardware commonalities [1]. The combined Raman / LIBS instrument has been recommended as the highest priority mineralogy instrument to be included in the rover's analytical laboratory for the following tasks: Analyse surface and sub-surface soil and rocks on Mars, identify organics in the search for life and determine soil origin & toxicity. The synergy of the system is evident: the Raman spectrometer is dedicated to molecular analysis of organics and minerals; the LIBS provides information on the sample's elemental composition. An international team, under ESA contract and with the leadership of TNO Science and Industry, has built and tested an Elegant Bread Board (EBB) of the combined Raman / LIBS instrument. The EBB comprises a specifically designed, extremely compact, spectrometer with high resolution over a large wavelength range, suitable for both Raman spectroscopy and LIBS measurements. The EBB also includes lasers, illumination and imaging optics as well as fibre optics for light transfer. A summary of the functional and environmental requirements together with a description of the optical design and its expected performance are described in [2]. The EBB was developed and constructed to verify the instruments' end-to-end functional performance with natural samples. The combined Raman / LIBS EBB realisation and test results of natural samples will be presented. For the Flight Model (FM) instrument, currently in the design phase, the Netherlands will be responsible for the design, development and verification of the

  5. Validation test of advanced technology for IPV nickel-hydrogen flight cells - Update

    Science.gov (United States)

    Smithrick, John J.; Hall, Stephen W.

    1992-01-01

    Individual pressure vessel (IPV) nickel-hydrogen technology was advanced at NASA Lewis and under Lewis contracts with the intention of improving cycle life and performance. One advancement was to use 26 percent potassium hydroxide (KOH) electrolyte to improve cycle life. Another advancement was to modify the state-of-the-art cell design to eliminate identified failure modes. The modified design is referred to as the advanced design. A breakthrough in the LEO cycle life of IPV nickel-hydrogen cells has been previously reported. The cycle life of boiler plate cells containing 26 percent KOH electrolyte was about 40,000 LEO cycles compared to 3,500 cycles for cells containing 31 percent KOH. The boiler plate test results are in the process of being validated using flight hardware and real time LEO testing. The primary function of the advanced cell is to store and deliver energy for long-term, LEO spacecraft missions. The new features of this design are: (1) use of 26 percent rather than 31 percent KOH electrolyte; (2) use of a patented catalyzed wall wick; (3) use of serrated-edge separators to facilitate gaseous oxygen and hydrogen flow within the cell, while still maintaining physical contact with the wall wick for electrolyte management; and (4) use of a floating rather than a fixed stack (state-of-the-art) to accommodate nickel electrode expansion due to charge/discharge cycling. The significant improvements resulting from these innovations are: extended cycle life; enhanced thermal, electrolyte, and oxygen management; and accommodation of nickel electrode expansion.

  6. Rapid identification of bacteria in positive blood culture by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Schmidt, V; Jarosch, A; März, P; Sander, C; Vacata, V; Kalka-Moll, W

    2012-03-01

    Blood culture is probably the most significant specimen used for the diagnosis of bacterial infections, especially for bloodstream infections. In the present study, we compared the resin-containing BD BACTEC™ Plus-Aerobic (Becton Dickinson), non-charcoal-containing BacT/Alert(®) SA (bioMérieux), and charcoal-containing BacT/Alert(®) FA (bioMérieux) blood culture bottles with direct identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 103 bacterial isolates, from clinical blood cultures, representing the most frequent 13 genera and 24 species were examined. Bacteria were extracted from positive blood culture broth by density centrifugation and then subjected to identification by MALDI-TOF MS using two different volumes and chemical treatments. Overall, correct identification by MALDI-TOF MS was obtained for the BD BACTEC™ Plus-Aerobic, BacT/Alert(®) SA, and BacT/Alert(®) FA blood culture bottles in 72%, 45.6%, and 23%, respectively, for gram-negative bacteria in 86.6%, 69.2%, and 47.1%, respectively, and for gram-positive bacteria in 60.0%, 28.8%, and 5.4%, respectively. The lack of identification was observed mainly with viridans streptococci. Depending on the blood culture bottles used in routine diagnostic procedures and the protocol for bacterial preparation, the applied MALDI-TOF MS represents an efficient and rapid method for direct bacterial identification.

  7. Direct identification of bacteria in blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: a new methodological approach.

    Science.gov (United States)

    Kroumova, Vesselina; Gobbato, Elisa; Basso, Elisa; Mucedola, Luca; Giani, Tommaso; Fortina, Giacomo

    2011-08-15

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently been demonstrated to be a powerful tool for the rapid identification of bacteria from growing colonies. In order to speed up the identification of bacteria, several authors have evaluated the usefulness of this MALDI-TOF MS technology for the direct and quick identification bacteria from positive blood cultures. The results obtained so far have been encouraging but have also shown some limitations, mainly related to the bacterial growth and to the presence of interference substances belonging to the blood cultures. In this paper, we present a new methodological approach that we have developed to overcome these limitations, based mainly on an enrichment of the sample into a growing medium before the extraction process, prior to mass spectrometric analysis. The proposed method shows important advantages for the identification of bacterial strains, yielding an increased identification score, which gives higher confidence in the results. Copyright © 2011 John Wiley & Sons, Ltd.

  8. Strategy for Comprehensive Profiling and Identification of Acidic Glycosphingolipids Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry.

    Science.gov (United States)

    Hu, Ting; Jia, Zhixin; Zhang, Jin-Lan

    2017-07-18

    Acidic glycosphingolipids (AGSLs), which mainly consist of ganglioside and sulfatide moieties, are highly concentrated in the central nervous system. Comprehensive profiling of AGSLs has historically been challenging because of their high complexity and the lack of standards. In this study, a novel strategy was developed to comprehensively profile AGSLs using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Ganglioside isomers with different glycan chains such as GD1a/GD1b were completely separated on a C18 column for the first time to our knowledge, facilitated by the addition of formic acid in the mobile phase. A mathematical model was established to predict the retention times (RTs) of all theoretically possible AGSLs on the basis of the good logarithmic relationship between the ceramide carbon numbers of the AGSLs in the reference material and their RTs. A data set was created of 571 theoretically possible AGSLs, including the ceramide carbon numbers, RTs, and high-resolution quasi-molecular ions. A novel fast identification strategy was established for global AGSL profiling by comparing the high-resolution quasi-molecular ions and RTs of the tested peaks to those in the data set of 571 AGSLs. Using this strategy, 199 AGSL candidates were identified in rat brain tissue. MS/MS fragments were further collected for these 199 candidates to confirm their identity as AGSLs. This novel strategy was employed to profile AGSLs in brain tissue samples from control rats and model rats with bilateral common carotid artery (2-VO) cerebral ischemia. Forty AGSLs were significantly different between the control and model groups, and these differences were further interpreted.

  9. Rapid Identification of Intact Staphylococcal Bacteriophages Using Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Dana Štveráková

    2018-04-01

    Full Text Available Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as a significant threat. Phage therapy could offer a safe and effective alternative to antibiotics. Phage preparations should comply with quality and safety requirements; therefore, it is important to develop efficient production control technologies. This study was conducted to develop and evaluate a rapid and reliable method for identifying staphylococcal bacteriophages, based on detecting their specific proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS profiling that is among the suggested methods for meeting the regulations of pharmaceutical authorities. Five different phage purification techniques were tested in combination with two MALDI-TOF MS matrices. Phages, either purified by CsCl density gradient centrifugation or as resuspended phage pellets, yielded mass spectra with the highest information value if ferulic acid was used as the MALDI matrix. Phage tail and capsid proteins yielded the strongest signals whereas the culture conditions had no effect on mass spectral quality. Thirty-seven phages from Myoviridae, Siphoviridae or Podoviridae families were analysed, including 23 siphophages belonging to the International Typing Set for human strains of S. aureus, as well as phages in preparations produced by Microgen, Bohemia Pharmaceuticals and MB Pharma. The data obtained demonstrate that MALDI-TOF MS can be used to effectively distinguish between Staphylococcus-specific bacteriophages.

  10. Preliminary Flight Results of the Microelectronics and Photonics Test Bed: NASA DR1773 Fiber Optic Data Bus Experiment

    Science.gov (United States)

    Jackson, George L.; LaBel, Kenneth A.; Marshall, Cheryl; Barth, Janet; Seidleck, Christina; Marshall, Paul

    1998-01-01

    NASA Goddard Spare Flight Center's (GSFC) Dual Rate 1773 (DR1773) Experiment on the Microelectronic and Photonic Test Bed (MPTB) has provided valuable information on the performance of the AS 1773 fiber optic data bus in the space radiation environment. Correlation of preliminary experiment data to ground based radiation test results show the AS 1773 bus is employable in future spacecraft applications requiring radiation tolerant communication links.

  11. Real Time Monitoring and Test Vector Generation for Improved Flight Safety, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — As the complexity of flight controllers grows so does the cost associated with verification and validation (V&V). Current-generation controllers are reaching...

  12. Flight Tests of a 0.13-Scale Model of the Convair XFY-1 Vertically Rising Airplane in a Setup Simulating that Proposed for Captive-Flight Tests in a Hangar, TED No. NACA DE 368

    Science.gov (United States)

    Lovell, Powell M., Jr.

    1953-01-01

    An experimental investigation has been conducted to determine the dynamic stability and control characteristics of a 0.13-scale free-flight model of the Convair XFY-1 airplane in test setups representing the setup proposed for use in the first flight tests of the full-scale airplane in the Moffett Field airship hangar. The investigation was conducted in two parts: first, tests with the model flying freely in an enclosure simulating the hangar, and second, tests with the model partially restrained by an overhead line attached to the propeller spinner and ground lines attached to the wing and tail tips. The results of the tests indicated that the airplane can be flown without difficulty in the Moffett Field airship hangar if it does not approach too close to the hangar walls. If it does approach too close to the walls, the recirculation of the propeller slipstream might cause sudden trim changes which would make smooth flight difficult for the pilot to accomplish. It appeared that the tethering system proposed by Convair could provide generally satisfactory restraint of large-amplitude motions caused by control failure or pilot error without interfering with normal flying or causing any serious instability or violent jerking motions as the tethering lines restrained the model.

  13. A method for crack profiles identification in eddy current testing by the multi-directional scan

    International Nuclear Information System (INIS)

    Kojima, Fumio; Ikeda, Takuya; Nguyen, Doung

    2006-01-01

    This paper is concerned with a method for identification of crack shape in conducting materials. Multi-directional scanning strategies using Eddy Current Testing is performed for sizing complex natural crackings. Two dimensional measurements by means of multi-directional scan are used in a output least square identifications. (author)

  14. Flight Tests of a Remaining Flying Time Prediction System for Small Electric Aircraft in the Presence of Faults

    Science.gov (United States)

    Hogge, Edward F.; Kulkarni, Chetan S.; Vazquez, Sixto L.; Smalling, Kyle M.; Strom, Thomas H.; Hill, Boyd L.; Quach, Cuong C.

    2017-01-01

    This paper addresses the problem of building trust in the online prediction of a battery powered aircraft's remaining flying time. A series of flight tests is described that make use of a small electric powered unmanned aerial vehicle (eUAV) to verify the performance of the remaining flying time prediction algorithm. The estimate of remaining flying time is used to activate an alarm when the predicted remaining time is two minutes. This notifies the pilot to transition to the landing phase of the flight. A second alarm is activated when the battery charge falls below a specified limit threshold. This threshold is the point at which the battery energy reserve would no longer safely support two repeated aborted landing attempts. During the test series, the motor system is operated with the same predefined timed airspeed profile for each test. To test the robustness of the prediction, half of the tests were performed with, and half were performed without, a simulated powertrain fault. The pilot remotely engages a resistor bank at a specified time during the test flight to simulate a partial powertrain fault. The flying time prediction system is agnostic of the pilot's activation of the fault and must adapt to the vehicle's state. The time at which the limit threshold on battery charge is reached is then used to measure the accuracy of the remaining flying time predictions. Accuracy requirements for the alarms are considered and the results discussed.

  15. The Ares I-1 Flight Test--Paving the Road for the Ares I Crew Launch Vehicle

    Science.gov (United States)

    Davis, Stephan R.; Tinker, Michael L.; Tuma, Meg

    2007-01-01

    In accordance with the U.S. Vision for Space Exploration and the nation's desire to again send humans to explore beyond Earth orbit, NASA has been tasked to send human beings to the moon, Mars, and beyond. It has been 30 years since the United States last designed and built a human-rated launch vehicle. NASA is now building the Ares I crew launch vehicle, which will loft the Orion crew exploration vehicle into orbit, and the Ares V cargo launch vehicle, which will launch the Lunar Surface Access Module and Earth departure stage to rendezvous Orion for missions to the moon. NASA has marshaled unique resources from the government and private sectors to perform the technically and programmatically complex work of delivering astronauts to orbit early next decade, followed by heavy cargo late next decade. Our experiences with Saturn and the Shuttle have taught us the value of adhering to sound systems engineering, such as the "test as you fly" principle, while applying aerospace best practices and lessons learned. If we are to fly humans safely aboard a launch vehicle, we must employ a variety of methodologies to reduce the technical, schedule, and cost risks inherent in the complex business of space transportation. During the Saturn development effort, NASA conducted multiple demonstration and verification flight tests to prove technology in its operating environment before relying upon it for human spaceflight. Less testing on the integrated Shuttle system did not reduce cost or schedule. NASA plans a progressive series of demonstration (ascent), verification (orbital), and mission flight tests to supplement ground research and high-altitude subsystem testing with real-world data, factoring the results of each test into the next one. In this way, sophisticated analytical models and tools, many of which were not available during Saturn and Shuttle, will be calibrated and we will gain confidence in their predictions, as we gain hands-on experience in operating the first

  16. Novel, Improved Sample Preparation for Rapid, Direct Identification from Positive Blood Cultures Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry

    OpenAIRE

    Schubert, Sören; Weinert, Kirsten; Wagner, Chris; Gunzl, Beatrix; Wieser, Andreas; Maier, Thomas; Kostrzewa, Markus

    2011-01-01

    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used for rapid and reliable identification of bacteria and yeast grown on agar plates. Moreover, MALDI-TOF MS also holds promise for bacterial identification from blood culture (BC) broths in hospital laboratories. The most important technical step for the identification of bacteria from positive BCs by MALDI-TOF MS is sample preparation to remove blood cells and host proteins. We present a m...

  17. AGARD Flight Test Techniques Series. Volume 2. Identification of Dynamic Systems

    Science.gov (United States)

    1985-01-01

    should not depend upon it to solve the problem autonomously. The analyst’s strong point is in formulating the problem; the computer’s strength is in...of derivation for the output-error method is to reduce the problem to the static form of Chapter 5. We will see that the dinamic system make- the

  18. Identification of barley and rye varieties using matrix- assisted laser desorption/ionisation time-of-flight mass spectrometry with neural networks

    DEFF Research Database (Denmark)

    Bloch, H.A.; Petersen, Marianne Kjerstine; Sperotto, Maria Maddalena

    2001-01-01

    developed, which combines analysis of alcohol-soluble wheat proteins (gliadins) using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry with neural networks. Here we have applied the same method for the identification of both barley (Hordeum vulgare L.) and rye (Secale cereale L.......) varieties. For barley, 95% of the mass spectra were correctly classified. This is an encouraging result, since in earlier experiments only a grouping into subsets of varieties was possible. However, the method was not useful in the classification of rye, due to the strong similarity between mass spectra...

  19. Flight service evaluation of composite components on the Bell Helicopter model 206L: Design, fabrication and testing

    Science.gov (United States)

    Zinberg, H.

    1982-01-01

    The design, fabrication, and testing phases of a program to obtain long term flight service experience on representative helicopter airframe structural components operating in typical commercial environments are described. The aircraft chosen is the Bell Helicopter Model 206L. The structural components are the forward fairing, litter door, baggage door, and vertical fin. The advanced composite components were designed to replace the production parts in the field and were certified by the FAA to be operable through the full flight envelope of the 206L. A description of the fabrication process that was used for each of the components is given. Static failing load tests on all components were done. In addition fatigue tests were run on four specimens that simulated the attachment of the vertical fin to the helicopter's tail boom.

  20. Evaluation of immittance testing for the identification of middle ear pathology in South African mineworkers.

    CSIR Research Space (South Africa)

    Habig, A

    2004-04-01

    Full Text Available The study evaluated immittance measures, specifically tympanometry and acoustic reflex testing, for the identification of middle ear pathology among South African mineworkers. The outcomes of medical history reviews, otoscopy, tympanometry, acoustic...

  1. Comparison of five tests for identification of Staphylococcus aureus from clinical samples

    NARCIS (Netherlands)

    A. Luijendijk (Ad); A.F. van Belkum (Alex); J.A.J.W. Kluytmans (Jan); H.A. Verbrugh (Henri)

    1996-01-01

    textabstractFive different laboratory tests for the identification of Staphylococcus aureus were compared. Analyses of 271 presumptive S. aureus strains, supplemented with 59 well-defined methicillin-resistant S. aureus (MRSA) isolates, were performed. Only the

  2. Microbial identification and automated antibiotic susceptibility testing directly from positive blood cultures using MALDI-TOF MS and VITEK 2.

    Science.gov (United States)

    Wattal, C; Oberoi, J K

    2016-01-01

    The study addresses the utility of Matrix Assisted Laser Desorption/Ionisation Time-Of-Flight mass spectrometry (MALDI-TOF MS) using VITEK MS and the VITEK 2 antimicrobial susceptibility testing (AST) system for direct identification (ID) and timely AST from positive blood culture bottles using a lysis-filtration method (LFM). Between July and December 2014, a total of 140 non-duplicate mono-microbial blood cultures were processed. An aliquot of positive blood culture broth was incubated with lysis buffer before the bacteria were filtered and washed. Micro-organisms recovered from the filter were first identified using VITEK MS and its suspension was used for direct AST by VITEK 2 once the ID was known. Direct ID and AST results were compared with classical methods using solid growth. Out of the 140 bottles tested, VITEK MS resulted in 70.7 % correct identification to the genus and/ or species level. For the 103 bottles where identification was possible, there was agreement in 97 samples (94.17 %) with classical culture. Compared to the routine method, the direct AST resulted in category agreement in 860 (96.5 %) of 891 bacteria-antimicrobial agent combinations tested. The results of direct ID and AST were available 16.1 hours before those of the standard approach on average. The combined use of VITEK MS and VITEK 2 directly on samples from positive blood culture bottles using a LFM technique can result in rapid and reliable ID and AST results in blood stream infections to result in early institution of targeted treatment. The combination of LFM and AST using VITEK 2 was found to expedite AST more reliably.

  3. Identification of Serum Biomarkers for Biliary Tract Cancers by a Proteomic Approach Based on Time-of-Flight Mass Spectrometry

    International Nuclear Information System (INIS)

    Wang, Wen-Jing; Xu, Wang-Hong; Liu, Cha-Zhen; Rashid, Asif; Cheng, Jia-Rong; Liao, Ping; Hu, Heng; Chu, Lisa W.; Gao, Yu-Tang; Yu, Kai; Hsing, Ann W.

    2010-01-01

    Biliary tract cancers (BTCs) are lethal malignancies currently lacking satisfactory methods for early detection and accurate diagnosis. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is a promising diagnostic tool for this disease. In this pilot study, sera samples from 50 BTCs and 30 cholelithiasis patients as well as 30 healthy subjects from a population-based case-control study were randomly grouped into training set (30 BTCs, 20 cholelithiasis and 20 controls), duplicate of training set, and blind set (20 BTCs, 10 cholelithiasis and 10 controls); all sets were analyzed on Immobilized Metal Affinity Capture ProteinChips via SELDI-TOF-MS. A decision tree classifier was built using the training set and applied to all test sets. The classification tree constructed with the 3,400, 4,502, 5,680, 7,598, and 11,242 mass-to-charge ratio (m/z) protein peaks had a sensitivity of 96.7% and a specificity of 85.0% when comparing BTCs with non-cancers. When applied to the duplicate set, sensitivity was 66.7% and specificity was 70.0%, while in the blind set, sensitivity was 95.0% and specificity was 75.0%. Positive predictive values of the training, duplicate, and blind sets were 82.9%, 62.5% and 79.2%, respectively. The agreement of the training and duplicate sets was 71.4% (Kappa = 0.43, u = 3.98, P < 0.01). The coefficient of variations based on 10 replicates of one sample for the five differential peaks were 15.8–68.8% for intensity and 0–0.05% for m/z. These pilot results suggest that serum protein profiling by SELDI-TOF-MS may be a promising approach for identifying BTCs but low assay reproducibility may limit its application in clinical practice

  4. Evaluation of a simple protein extraction method for species identification of clinically relevant staphylococci by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    Science.gov (United States)

    Matsuda, Naoto; Matsuda, Mari; Notake, Shigeyuki; Yokokawa, Hirohide; Kawamura, Yoshiaki; Hiramatsu, Keiichi; Kikuchi, Ken

    2012-12-01

    In clinical microbiology, bacterial identification is labor-intensive and time-consuming. A solution for this problem is the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In this study, we evaluated a modified protein extraction method of identification performed on target plates (on-plate extraction method) with MALDI-TOF (Bruker Microflex LT with Biotyper version 3.0) and compared it to 2 previously described methods: the direct colony method and a standard protein extraction method (standard extraction method). We evaluated the species of 273 clinical strains and 14 reference strains of staphylococci. All isolates were characterized using the superoxide dismutase A sequence as a reference. For the species identification, the on-plate, standard extraction, and direct colony methods identified 257 isolates (89.5%), 232 isolates (80.8%), and 173 isolates (60.2%), respectively, with statistically significant differences among the three methods (P extraction method is at least as good as standard extraction in identification rate and has the advantage of a shorter processing time.

  5. Rapid identification and typing of Yersinia pestis and other Yersinia species by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.

    Science.gov (United States)

    Ayyadurai, Saravanan; Flaudrops, Christophe; Raoult, Didier; Drancourt, Michel

    2010-11-12

    Accurate identification is necessary to discriminate harmless environmental Yersinia species from the food-borne pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis and from the group A bioterrorism plague agent Yersinia pestis. In order to circumvent the limitations of current phenotypic and PCR-based identification methods, we aimed to assess the usefulness of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) protein profiling for accurate and rapid identification of Yersinia species. As a first step, we built a database of 39 different Yersinia strains representing 12 different Yersinia species, including 13 Y. pestis isolates representative of the Antiqua, Medievalis and Orientalis biotypes. The organisms were deposited on the MALDI-TOF plate after appropriate ethanol-based inactivation, and a protein profile was obtained within 6 minutes for each of the Yersinia species. When compared with a 3,025-profile database, every Yersinia species yielded a unique protein profile and was unambiguously identified. In the second step of analysis, environmental and clinical isolates of Y. pestis (n = 2) and Y. enterocolitica (n = 11) were compared to the database and correctly identified. In particular, Y. pestis was unambiguously identified at the species level, and MALDI-TOF was able to successfully differentiate the three biotypes. These data indicate that MALDI-TOF can be used as a rapid and accurate first-line method for the identification of Yersinia isolates.

  6. Short communication: Identification of subclinical cow mastitis pathogens in milk by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Barreiro, J R; Ferreira, C R; Sanvido, G B; Kostrzewa, M; Maier, T; Wegemann, B; Böttcher, V; Eberlin, M N; dos Santos, M V

    2010-12-01

    Subclinical mastitis is a common and easily disseminated disease in dairy herds. Its routine diagnosis via bacterial culture and biochemical identification is a difficult and time-consuming process. In this work, we show that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows bacterial identification with high confidence and speed (1 d for bacterial growth and analysis). With the use of MALDI-TOF MS, 33 bacterial culture isolates from milk of different dairy cows from several farms were analyzed, and the results were compared with those obtained by classical biochemical methods. This proof-of-concept case demonstrates the reliability of MALDI-TOF MS bacterial identification, and its increased selectivity as illustrated by the additional identification of coagulase-negative Staphylococcus species and mixed bacterial cultures. Matrix-assisted laser desorption-ionization mass spectrometry considerably accelerates the diagnosis of mastitis pathogens, especially in cases of subclinical mastitis. More immediate and efficient animal management strategies for mastitis and milk quality control in the dairy industry can therefore be applied. Copyright © 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  7. Identification of Cronobacter species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with an optimized analysis method.

    Science.gov (United States)

    Wang, Qi; Zhao, Xiao-Juan; Wang, Zi-Wei; Liu, Li; Wei, Yong-Xin; Han, Xiao; Zeng, Jing; Liao, Wan-Jin

    2017-08-01

    Rapid and precise identification of Cronobacter species is important for foodborne pathogen detection, however, commercial biochemical methods can only identify Cronobacter strains to genus level in most cases. To evaluate the power of mass spectrometry based on matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS) for Cronobacter species identification, 51 Cronobacter strains (eight reference and 43 wild strains) were identified by both MALDI-TOF MS and 16S rRNA gene sequencing. Biotyper RTC provided by Bruker identified all eight reference and 43 wild strains as Cronobacter species, which demonstrated the power of MALDI-TOF MS to identify Cronobacter strains to genus level. However, using the Bruker's database (6903 main spectra products) and Biotyper software, the MALDI-TOF MS analysis could not identify the investigated strains to species level. When MALDI-TOF MS analysis was performed using the combined in-house Cronobacter database and Bruker's database, bin setting, and unweighted pair group method with arithmetic mean (UPGMA) clustering, all the 51 strains were clearly identified into six Cronobacter species and the identification accuracy increased from 60% to 100%. We demonstrated that MALDI-TOF MS was reliable and easy-to-use for Cronobacter species identification and highlighted the importance of establishing a reliable database and improving the current data analysis methods by integrating the bin setting and UPGMA clustering. Copyright © 2017. Published by Elsevier B.V.

  8. Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for the identification of ceratopogonid and culicid larvae.

    Science.gov (United States)

    Steinmann, I C; Pflüger, V; Schaffner, F; Mathis, A; Kaufmann, C

    2013-03-01

    Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the rapid identification of ceratopogonid larvae. Optimal sample preparation as evaluated with laboratory-reared biting midges Culicoides nubeculosus was the homogenization of gut-less larvae in 10% formic acid, and analysis of 0.2 mg/ml crude protein homogenate mixed with SA matrix at a ratio of 1:1.5. Using 5 larvae each of 4 ceratopogonid species (C. nubeculosus, C. obsoletus, C. decor, and Dasyhelea sp.) and of 2 culicid species (Aedes aegypti, Ae. japonicus), biomarker mass sets between 27 and 33 masses were determined. In a validation study, 67 larvae belonging to the target species were correctly identified by automated database-based identification (91%) or manual full comparison (9%). Four specimens of non-target species did not yield identification. As anticipated for holometabolous insects, the biomarker mass sets of adults cannot be used for the identification of larvae, and vice versa, because they share only very few similar masses as shown for C. nubeculosus, C. obsoletus, and Ae. japonicus. Thus, protein profiling by MALDI-TOF as a quick, inexpensive and accurate alternative tool is applicable to identify insect larvae of vector species collected in the field.

  9. Development of a rapid and simplified protocol for direct bacterial identification from positive blood cultures by using matrix assisted laser desorption ionization time-of- flight mass spectrometry.

    Science.gov (United States)

    Jakovljev, Aleksandra; Bergh, Kåre

    2015-11-06

    Bloodstream infections represent serious conditions carrying a high mortality and morbidity rate. Rapid identification of microorganisms and prompt institution of adequate antimicrobial therapy is of utmost importance for a successful outcome. Aiming at the development of a rapid, simplified and efficient protocol, we developed and compared two in-house preparatory methods for the direct identification of bacteria from positive blood culture flasks (BD BACTEC FX system) by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS). Both methods employed saponin and distilled water for erythrocyte lysis. In method A the cellular pellet was overlaid with formic acid on the MALDI TOF target plate for protein extraction, whereas in method B the pellet was exposed to formic acid followed by acetonitrile prior to placing on the target plate. Best results were obtained by method A. Direct identification was achieved for 81.9 % and 65.8 % (50.3 % and 26.2 % with scores >2.0) of organisms by method A and method B, respectively. Overall concordance with final identification was 100 % to genus and 97.9 % to species level. By applying a lower cut-off score value, the levels of identification obtained by method A and method B increased to 89.3 % and 77.8 % of organisms (81.9 % and 65.8 % identified with scores >1.7), respectively. Using the lowered score criteria, concordance with final results was obtained for 99.3 % of genus and 96.6 % of species identifications. The reliability of results, rapid performance (approximately 25 min) and applicability of in-house method A have contributed to implementation of this robust and cost-effective method in our laboratory.

  10. Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper™ and time of flight mass spectrometry.

    Directory of Open Access Journals (Sweden)

    Jen Kok

    Full Text Available Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS is a novel method for the direct identification of bacteria from blood culture broths. We evaluate for the first time, the performance of the MALDI Sepsityper™ Kit and MS for the identification of bacteria compared to standard phenotypic methods using the manufacturer's specified bacterial identification criteria (spectral scores ≥1.700-1.999 and ≥2.000 indicated identification to genus and species level, respectively. Five hundred and seven positive blood culture broths were prospectively examined, of which 379 (74.8%; 358 monomicrobial, 21 polymicrobial were identified by MALDI-TOF MS; 195 (100% and 132 (67.7% of 195 gram-positive; and 163 (100% and 149 (91.4% of 163 gram-negative organisms from monomicrobial blood cultures were correctly identified to genus and species level, respectively. Spectral scores <1.700 (no identification were obtained in 128/507 (25.2% positive blood culture broths, including 31.6% and 32.3% of gram-positive and polymicrobial blood cultures, respectively. Significantly more gram-negative organisms were identified compared to gram-positive organisms at species level (p<0.0001. Five blood cultures were misidentified, but at species level only; including four monomicrobial blood cultures with Streptococcus oralis/mitis that were misidentified as Streptococcus pneumoniae. Positive predictive values for the direct identification of both gram-positive and gram-negative bacteria from monomicrobial blood culture broths to genus level were 100%. A diagnostic algorithm for positive blood culture broths that incorporates gram staining and MALDI-TOF MS should identify the majority of pathogens, particularly to genus level.

  11. Flight Tests of a 0.13-Scale Model of the Convair XFY-1 Vertically Rising Airplane with the Lower Vertical Tail Removed, TED No.DE 368

    Science.gov (United States)

    Lovell, Powell M., Jr.

    1954-01-01

    An experimental investigation has been conducted to determine the dynamic stability and control characteristics in hovering and transition flight of a 0.13-scale flying model of the Convair XFY-1 vertically rising airplane with the lower vertical tail removed. The purpose of the tests was to obtain a general indication of the behavior of a vertically rising airplane of the same general type as the XFY-1 but without a lower vertical tail in order to simplify power-off belly landings in an emergency. The model was flown satisfactorily in hovering flight and in the transition from hovering to normal unstalled forward flight (angle of attack approximately 30deg). From an angle of attack of about 30 down to the lowest angle of attack covered in the flight tests (approximately 15deg) the model became progressively more difficult to control. These control difficulties were attributed partly to a lightly damped Dutch roll oscillation and partly to the fact that the control deflections required for hovering and transition flight were too great for smooth flight at high speeds. In the low-angle-of-attack range not covered in the flight tests, force tests have indicated very low static directional stability which would probably result in poor flight characteristics. It appears, therefore, that the attainment of satisfactory directional stability, at angles of attack less than 10deg, rather than in the hovering and transition ranges of flight is the critical factor in the design of the vertical tail for such a configuration.

  12. Kaon identification and Search for Lepton Number Violation in $K^{\\pm}$ decay-in-flight experiments at CERN

    CERN Document Server

    Massri, Karim; Goudzovski, Evgueni

    A search for the Lepton Number Violating decay $K^{\\pm} \\to \\pi^{\\pm} \\mu^{\\pm} \\mu^{\\pm}$ has been performed using the data collected by the NA48/2 experiment in 2003 and 2004. The signal event selection, the background rejection, the evaluation of the muon identifcation efficiency and the statistical methods used for the data interpretation are presented. Based on $1.8 \\times 10 ^{11}$ kaon decays in the fiducial volume and using several models for the signal, upper limits for the branching fraction B($K^{\\pm} \\to \\pi^{\\pm} \\mu^{\\pm} \\mu^{\\pm}$) of the order of $10^{-10} $ have been obtained for 90%, 95% and 99% confidence levels, improving the previous best limit by one order of magnitude. The Cherenkov differential counter used for kaon identification in the NA62 experiment, equipped with approximately 30% of the photo-detectors, was installed and tested during a Technical Run in 2012. The counter's ability of distinguishing between kaons and pions has been validated via pressure scan procedure. The da...

  13. suitability of vitek 2 system in identification and susceptibility testing

    African Journals Online (AJOL)

    2014-04-01

    Apr 1, 2014 ... susceptibility testing (AST) of bacteria from blood are ... This process ... This was a method validation study carried out at .... testing: Of the 19 cultures with AST by direct Vitek, three had discrepancies for some antibiotics.

  14. Strain Gage Load Calibration of the Wing Interface Fittings for the Adaptive Compliant Trailing Edge Flap Flight Test

    Science.gov (United States)

    Miller, Eric J.; Holguin, Andrew C.; Cruz, Josue; Lokos, William A.

    2014-01-01

    This is the presentation to follow conference paper of the same name. The adaptive compliant trailing edge (ACTE) flap experiment safety of flight requires that the flap to wing interface loads be sensed and monitored in real time to ensure that the wing structural load limits are not exceeded. This paper discusses the strain gage load calibration testing and load equation derivation methodology for the ACTE interface fittings. Both the left and right wing flap interfaces will be monitored and each contains four uniquely designed and instrumented flap interface fittings. The interface hardware design and instrumentation layout are discussed. Twenty one applied test load cases were developed using the predicted in-flight loads for the ACTE experiment.

  15. Role of failure-mechanism identification in accelerated testing

    Science.gov (United States)

    Hu, J. M.; Barker, D.; Dasgupta, A.; Arora, A.

    1993-01-01

    Accelerated life testing techniques provide a short-cut method to investigate the reliability of electronic devices with respect to certain dominant failure mechanisms that occur under normal operating conditions. However, accelerated tests have often been conducted without knowledge of the failure mechanisms and without ensuring that the test accelerated the same mechanism as that observed under normal operating conditions. This paper summarizes common failure mechanisms in electronic devices and packages and investigates possible failure mechanism shifting during accelerated testing.

  16. Identification of Radioactive Pilot-Plant test requirements

    Energy Technology Data Exchange (ETDEWEB)

    Powell, W.J.; Riebling, E.F.

    1995-05-09

    Radioactive Pilot-Plant testing needs and alternatives are evaluated for enhanced Sludge Washing and High and Low-Level Vitrification efforts. Also investigated was instrument and equipment testing needs associated with the vitrification and retrieval process. The scope of this document is to record the existing March 1994 letter report for future use. A structured Kepner-Trego{trademark} decision analysis process was used to assist analysis of the testing needs. This analysis provided various combinations of laboratory and radioactive (hot) and cold pilot testing options associated with the above need areas. Recommendations for testing requirements were made.

  17. Identification of Radioactive Pilot-Plant test requirements

    International Nuclear Information System (INIS)

    Powell, W.J.; Riebling, E.F.

    1995-01-01

    Radioactive Pilot-Plant testing needs and alternatives are evaluated for enhanced Sludge Washing and High and Low-Level Vitrification efforts. Also investigated was instrument and equipment testing needs associated with the vitrification and retrieval process. The scope of this document is to record the existing March 1994 letter report for future use. A structured Kepner-Trego trademark decision analysis process was used to assist analysis of the testing needs. This analysis provided various combinations of laboratory and radioactive (hot) and cold pilot testing options associated with the above need areas. Recommendations for testing requirements were made

  18. Concordance between hypoxic challenge testing and predictive equations for hypoxic flight assessment in chronic obstructive pulmonary disease patients prior to air travel

    Directory of Open Access Journals (Sweden)

    Mohie Aldeen Abd Alzaher Khalifa

    2016-10-01

    Conclusions: The present study supports on-HCT as a reliable, on-invasive and continuous methods determining the requirement for in-flight O2 are relatively constant. Predictive equations considerably overestimate the need for in-flight O2 compared to hypoxic inhalation test. Predictive equations are cheap, readily available methods of flight assessment, but this study shows poor agreement between their predictions and the measured individual hypoxic responses during HCT.

  19. A Unique Outside Neutron and Gamma Ray Instrumentation Development Test Facility at NASA's Goddard Space Flight Center

    Science.gov (United States)

    Bodnarik, J.; Evans, L.; Floyd, S.; Lim, L.; McClanahan, T.; Namkung, M.; Parsons, A.; Schweitzer, J.; Starr, R.; Trombka, J.

    2010-01-01

    An outside neutron and gamma ray instrumentation test facility has been constructed at NASA's Goddard Space Flight Center (GSFC) to evaluate conceptual designs of gamma ray and neutron systems that we intend to propose for future planetary lander and rover missions. We will describe this test facility and its current capabilities for operation of planetary in situ instrumentation, utilizing a l4 MeV pulsed neutron generator as the gamma ray excitation source with gamma ray and neutron detectors, in an open field with the ability to remotely monitor and operate experiments from a safe distance at an on-site building. The advantage of a permanent test facility with the ability to operate a neutron generator outside and the flexibility to modify testing configurations is essential for efficient testing of this type of technology. Until now, there have been no outdoor test facilities for realistically testing neutron and gamma ray instruments planned for solar system exploration

  20. Flight Test of a Technology Transparent Light Concentration Panel on SMEX/WIRE

    Science.gov (United States)

    Stern, Theodore G.; Lyons, John

    2000-01-01

    A flight experiment has demonstrated a modular solar concentrator that can be used as a direct substitute replacement for planar photovoltaic panels in spacecraft solar arrays. The Light Concentrating Panel (LCP) uses an orthogrid arrangement of composite mirror strips to form an array of rectangular mirror troughs that reflect light onto standard, high-efficiency solar cells at a concentration ratio of approximately 3:1. The panel area, mass, thickness, and pointing tolerance has been shown to be similar to a planar array using the same cells. Concentration reduces the panel's cell area by 2/3, which significantly reduces the cost of the panel. An opportunity for a flight experiment module arose on NASA's Small Explorer / Wide-Field Infrared Explorer (SMEX/WIRE) spacecraft, which uses modular solar panel modules integrated into a solar panel frame structure. The design and analysis that supported implementation of the LCP as a flight experiment module is described. Easy integration into the existing SMEX-LITE wing demonstrated the benefits of technology transparency. Flight data shows the stability of the LCP module after nearly one year in Low Earth Orbit.

  1. Flight Testing of Novel Compliant Spines for Passive Wing Morphing on Ornithopters

    Science.gov (United States)

    Wissa, Aimy; Guerreiro, Nelson; Grauer, Jared; Altenbuchner, Cornelia; Hubbard, James E., Jr.; Tummala, Yashwanth; Frecker, Mary; Roberts, Richard

    2013-01-01

    Unmanned Aerial Vehicles (UAVs) are proliferating in both the civil and military markets. Flapping wing UAVs, or ornithopters, have the potential to combine the agility and maneuverability of rotary wing aircraft with excellent performance in low Reynolds number flight regimes. The purpose of this paper is to present new free flight experimental results for an ornithopter equipped with one degree of freedom (1DOF) compliant spines that were designed and optimized in terms of mass, maximum von-Mises stress, and desired wing bending deflections. The spines were inserted in an experimental ornithopter wing spar in order to achieve a set of desired kinematics during the up and down strokes of a flapping cycle. The ornithopter was flown at Wright Patterson Air Force Base in the Air Force Research Laboratory Small Unmanned Air Systems (SUAS) indoor flight facility. Vicon motion tracking cameras were used to track the motion of the vehicle for five different wing configurations. The effect of the presence of the compliant spine on wing kinematics and leading edge spar deflection during flight is presented. Results show that the ornithopter with the compliant spine inserted in its wing reduced the body acceleration during the upstroke which translates into overall lift gains.

  2. The Author’s Guide to Writing Air Force Flight Test Center Technical Reports

    Science.gov (United States)

    2009-08-01

    arguments about how bad is bad and how good is good; this table should apply in most cases. If you feel you have an exception to the rating table... IFR instrument flight rules --- ILS instrument landing system --- IMC instrument meteorological conditions --- IMU inertial measurement

  3. Towards Flight Testing of Remotely Controlled Surfkites for Wind Energy Generation

    NARCIS (Netherlands)

    Lansdorp, B.; Ruiterkamp, R.; Ockels, W.

    2007-01-01

    Non-powered flight vehicles such as kites can provide a means of transmitting wind energy from higher altitudes to the ground via tethers. Although there is increased world wide interest for systems to extract wind energy from higher altitudes with kites, research into kite properties such as the

  4. Implementation of Dryden Continuous Turbulence Model into Simulink for LSA-02 Flight Test Simulation

    Science.gov (United States)

    Ichwanul Hakim, Teuku Mohd; Arifianto, Ony

    2018-04-01

    Turbulence is a movement of air on small scale in the atmosphere that caused by instabilities of pressure and temperature distribution. Turbulence model is integrated into flight mechanical model as an atmospheric disturbance. Common turbulence model used in flight mechanical model are Dryden and Von Karman model. In this minor research, only Dryden continuous turbulence model were made. Dryden continuous turbulence model has been implemented, it refers to the military specification MIL-HDBK-1797. The model was implemented into Matlab Simulink. The model will be integrated with flight mechanical model to observe response of the aircraft when it is flight through turbulence field. The turbulence model is characterized by multiplying the filter which are generated from power spectral density with band-limited Gaussian white noise input. In order to ensure that the model provide a good result, model verification has been done by comparing the implemented model with the similar model that is provided in aerospace blockset. The result shows that there are some difference for 2 linear velocities (vg and wg), and 3 angular rate (pg, qg and rg). The difference is instantly caused by different determination of turbulence scale length which is used in aerospace blockset. With the adjustment of turbulence length in the implemented model, both model result the similar output.

  5. COBALT: A GN&C Payload for Testing ALHAT Capabilities in Closed-Loop Terrestrial Rocket Flights

    Science.gov (United States)

    Carson, John M., III; Amzajerdian, Farzin; Hines, Glenn D.; O'Neal, Travis V.; Robertson, Edward A.; Seubert, Carl; Trawny, Nikolas

    2016-01-01

    The COBALT (CoOperative Blending of Autonomous Landing Technology) payload is being developed within NASA as a risk reduction activity to mature, integrate and test ALHAT (Autonomous precision Landing and Hazard Avoidance Technology) systems targeted for infusion into near-term robotic and future human space flight missions. The initial COBALT payload instantiation is integrating the third-generation ALHAT Navigation Doppler Lidar (NDL) sensor, for ultra high-precision velocity plus range measurements, with the passive-optical Lander Vision System (LVS) that provides Terrain Relative Navigation (TRN) global-position estimates. The COBALT payload will be integrated onboard a rocket-propulsive terrestrial testbed and will provide precise navigation estimates and guidance planning during two flight test campaigns in 2017 (one open-loop and closed- loop). The NDL is targeting performance capabilities desired for future Mars and Moon Entry, Descent and Landing (EDL). The LVS is already baselined for TRN on the Mars 2020 robotic lander mission. The COBALT platform will provide NASA with a new risk-reduction capability to test integrated EDL Guidance, Navigation and Control (GN&C) components in closed-loop flight demonstrations prior to the actual mission EDL.

  6. Flight Testing a Real-Time Hazard Detection System for Safe Lunar Landing on the Rocket-Powered Morpheus Vehicle

    Science.gov (United States)

    Trawny, Nikolas; Huertas, Andres; Luna, Michael E.; Villalpando, Carlos Y.; Martin, Keith E.; Carson, John M.; Johnson, Andrew E.; Restrepo, Carolina; Roback, Vincent E.

    2015-01-01

    The Hazard Detection System (HDS) is a component of the ALHAT (Autonomous Landing and Hazard Avoidance Technology) sensor suite, which together provide a lander Guidance, Navigation and Control (GN&C) system with the relevant measurements necessary to enable safe precision landing under any lighting conditions. The HDS consists of a stand-alone compute element (CE), an Inertial Measurement Unit (IMU), and a gimbaled flash LIDAR sensor that are used, in real-time, to generate a Digital Elevation Map (DEM) of the landing terrain, detect candidate safe landing sites for the vehicle through Hazard Detection (HD), and generate hazard-relative navigation (HRN) measurements used for safe precision landing. Following an extensive ground and helicopter test campaign, ALHAT was integrated onto the Morpheus rocket-powered terrestrial test vehicle in March 2014. Morpheus and ALHAT then performed five successful free flights at the simulated lunar hazard field constructed at the Shuttle Landing Facility (SLF) at Kennedy Space Center, for the first time testing the full system on a lunar-like approach geometry in a relevant dynamic environment. During these flights, the HDS successfully generated DEMs, correctly identified safe landing sites and provided HRN measurements to the vehicle, marking the first autonomous landing of a NASA rocket-powered vehicle in hazardous terrain. This paper provides a brief overview of the HDS architecture and describes its in-flight performance.

  7. X-51A Scramjet Demonstrator Program: Waverider Ground and Flight Test

    Science.gov (United States)

    2013-11-01

    identification NASA National Aeronautics and Space Administration Scramjet supersonic ramjet (scramjet) TM telemetry 3 INTRODUCTION The fourth and final...aerodynamic parameter identification (PID) maneuvers were to be performed at Mach numbers 5, 4, 3, and 2. After almost 5 minutes of descent, the X-51A...resolve the issue and lineup for a second attempt. The F-15 successfully took off without major impact to the mission timing. As noted earlier, with the

  8. Acquisition and use of Orlando, Florida and Continental Airbus radar flight test data

    Science.gov (United States)

    Eide, Michael C.; Mathews, Bruce

    1992-01-01

    Westinghouse is developing a lookdown pulse Doppler radar for production as the sensor and processor of a forward looking hazardous windshear detection and avoidance system. A data collection prototype of that product was ready for flight testing in Orlando to encounter low level windshear in corroboration with the FAA-Terminal Doppler Weather Radar (TDWR). Airborne real-time processing and display of the hazard factor were demonstrated with TDWR facilitated intercepts and penetrations of over 80 microbursts in a three day period, including microbursts with hazard factors in excess of .16 (with 500 ft. PIREP altitude loss) and the hazard factor display at 6 n.mi. of a visually transparent ('dry') microburst with TDWR corroborated outflow reflectivities of +5 dBz. Range gated Doppler spectrum data was recorded for subsequent development and refinement of hazard factor detection and urban clutter rejection algorithms. Following Orlando, the data collection radar was supplemental type certified for in revenue service on a Continental Airlines Airbus in an automatic and non-interferring basis with its ARINC 708 radar to allow Westinghouse to confirm its understanding of commercial aircraft installation, interface realities, and urban airport clutter. A number of software upgrades, all of which were verified at the Receiver-Transmitter-Processor (RTP) hardware bench with Orlando microburst data to produce desired advanced warning hazard factor detection, included some preliminary loads with automatic (sliding window average hazard factor) detection and annunciation recording. The current (14-APR-92) configured software is free from false and/or nuisance alerts (CAUTIONS, WARNINGS, etc.) for all take-off and landing approaches, under 2500 ft. altitude to weight-on-wheels, into all encountered airports, including Newark (NJ), LAX, Denver, Houston, Cleveland, etc. Using the Orlando data collected on hazardous microbursts, Westinghouse has developed a lookdown pulse Doppler

  9. Identification of irradiated foodstuffs: results of a European test intercomparison

    International Nuclear Information System (INIS)

    Raffi, J.; Belliardo, J.-J.; Agnel, J.-P.; Vincent, P.

    1993-01-01

    The results of an intercomparison, organized by the Community Bureau of Reference (Commission of the European Communities), on the use of Electron Spin Resonance spectroscopy for the identification of irradiated food are presented. A qualitative intercomparison was carried out using beef and trout bones, sardine scales, pistachio nut shells, dried grapes and papaya. Protocols are proposed for meat bones, fish bones (with some restrictions) and fruits such as dried grapes and papaya. The protocol for pistachio nuts and fruits such as strawberries is more complicated and further research is needed prior the organization of future intercomparisons. A quantitative intercomparison on poultry bones was also organized. Laboratories were able to distinguish between chicken bones irradiated at 1 to 3 kGy or 7 to 10 kGy. (author)

  10. Aquatic toxicity testing for hazard identification of engineered nanoparticles

    DEFF Research Database (Denmark)

    Sørensen, Sara Nørgaard

    the traditionally applied, and determination of different exposure fractions such as the concentration of dissolved ions from ENPs and body burdens. Although these approaches are scientifically exploratory by nature, the aim is to generate data applicable for regulatory hazard identification of ENPs. The focus has......Within the last few decades, major advances in the field of nanotechnology have enabled production of engineered nanoparticles (ENPs) for various applications and consumer products already available on the market. ENPs may exhibit unique and novel properties compared to their bulk counterparts...... and the response axes. The actual exposure experienced by organisms may not be reflected by the ENPconcentration in medium, commonly applied as the exposure metric, and the responses of organisms may result from various toxic and non-toxic mechanisms occurring simultaneously. In this thesis, the challenges related...

  11. Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    Directory of Open Access Journals (Sweden)

    Carole Cassagne

    Full Text Available BACKGROUND: MALDI-TOF MS recently emerged as a valuable identification tool for bacteria and yeasts and revolutionized the daily clinical laboratory routine. But it has not been established for routine mould identification. This study aimed to validate a standardized procedure for MALDI-TOF MS-based mould identification in clinical laboratory. MATERIALS AND METHODS: First, pre-extraction and extraction procedures were optimized. With this standardized procedure, a 143 mould strains reference spectra library was built. Then, the mould isolates cultured from sequential clinical samples were prospectively subjected to this MALDI-TOF MS based-identification assay. MALDI-TOF MS-based identification was considered correct if it was concordant with the phenotypic identification; otherwise, the gold standard was DNA sequence comparison-based identification. RESULTS: The optimized procedure comprised a culture on sabouraud-gentamicin-chloramphenicol agar followed by a chemical extraction of the fungal colonies with formic acid and acetonitril. The identification was done using a reference database built with references from at least four culture replicates. For five months, 197 clinical isolates were analyzed; 20 were excluded because they were not identified at the species level. MALDI-TOF MS-based approach correctly identified 87% (154/177 of the isolates analyzed in a routine clinical laboratory activity. It failed in 12% (21/177, whose species were not represented in the reference library. MALDI-TOF MS-based identification was correct in 154 out of the remaining 156 isolates. One Beauveria bassiana was not identified and one Rhizopus oryzae was misidentified as Mucor circinelloides. CONCLUSIONS: This work's seminal finding is that a standardized procedure can also be used for MALDI-TOF MS-based identification of a wide array of clinically relevant mould species. It thus makes it possible to identify moulds in the routine clinical laboratory setting

  12. Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper™ and time of flight mass spectrometry.

    Science.gov (United States)

    Kok, Jen; Thomas, Lee C; Olma, Thomas; Chen, Sharon C A; Iredell, Jonathan R

    2011-01-01

    Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a novel method for the direct identification of bacteria from blood culture broths. We evaluate for the first time, the performance of the MALDI Sepsityper™ Kit and MS for the identification of bacteria compared to standard phenotypic methods using the manufacturer's specified bacterial identification criteria (spectral scores ≥1.700-1.999 and ≥2.000 indicated identification to genus and species level, respectively). Five hundred and seven positive blood culture broths were prospectively examined, of which 379 (74.8%; 358 monomicrobial, 21 polymicrobial) were identified by MALDI-TOF MS; 195 (100%) and 132 (67.7%) of 195 gram-positive; and 163 (100%) and 149 (91.4%) of 163 gram-negative organisms from monomicrobial blood cultures were correctly identified to genus and species level, respectively. Spectral scores blood culture broths, including 31.6% and 32.3% of gram-positive and polymicrobial blood cultures, respectively. Significantly more gram-negative organisms were identified compared to gram-positive organisms at species level (pblood cultures were misidentified, but at species level only; including four monomicrobial blood cultures with Streptococcus oralis/mitis that were misidentified as Streptococcus pneumoniae. Positive predictive values for the direct identification of both gram-positive and gram-negative bacteria from monomicrobial blood culture broths to genus level were 100%. A diagnostic algorithm for positive blood culture broths that incorporates gram staining and MALDI-TOF MS should identify the majority of pathogens, particularly to genus level.

  13. Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems.

    Science.gov (United States)

    Chen, Jonathan H K; Ho, Pak-Leung; Kwan, Grace S W; She, Kevin K K; Siu, Gilman K H; Cheng, Vincent C C; Yuen, Kwok-Yung; Yam, Wing-Cheong

    2013-06-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of bacteria and fungi was recently introduced in microbiology laboratories. This technology could greatly improve the clinical management of patients and guidance for chemotherapy. In this study, we used a commercial MALDI Sepsityper extraction method to evaluate the performance of two commercial MALDI-TOF MS systems, the Vitek MS IVD (bioMérieux) and the Microflex LT Biotyper (Bruker Daltonics) for direct bacterial identification in positive blood cultures. In 181 monomicrobial cultures, both systems generated genus to species level identifications for >90% of the specimens (Biotyper, 177/181 [97.8%]; Vitek MS IVD, 167/181 [92.3%]). Overall, the Biotyper system generated significantly more accurate identifications than the Vitek MS IVD system (P = 0.016; 177 versus 167 out of 181 specimens). The Biotyper system identified the minority species among polymicrobial blood cultures. We also compared the performance of an in-house extraction method with that of the Sepsityper on both MALDI-TOF MS systems. The in-house method generated more correct identifications at the genus level than the Sepsityper (96.7% versus 93.5%) on the Biotyper system, whereas the two methods exhibited the same performance level (88.0% versus 88.0%) on the Vitek MS IVD system. Our study confirmed the practical advantages of MALDI-TOF MS, and our in-house extraction method reduced the reagent cost to $1 per specimen, with a shorter turnaround time of 3 h, which is highly cost-effective for a diagnostic microbiology service.

  14. Radiation Isotope Identification Device (RIIDs) Field Test and Evaluation Campaign

    International Nuclear Information System (INIS)

    Christopher Hodge, Raymond Keegan

    2007-01-01

    Handheld, backpack, and mobile sensors are elements of the Global Nuclear Detection System for the interdiction and control of illicit radiological and nuclear materials. They are used by the U.S. Department of Homeland Security (DHS) and other government agencies and organizations in various roles for border protection, law enforcement, and nonproliferation monitoring. In order to systematically document the operational performance of the common commercial off-the-shelf portable radiation detection systems, the DHS Domestic Nuclear Detection Office conducted a test and evaluation campaign conducted at the Nevada Test Site from January 18 to February 27, 2006. Named 'Anole', it was the first test of its kind in terms of technical design and test complexities. The Anole test results offer users information for selecting appropriate mission-specific portable radiation detection systems. The campaign also offered manufacturers the opportunity to submit their equipment for independent operationally relevant testing to subsequently improve their detector performance. This paper will present the design, execution, and methodologies of the DHS Anole portable radiation detection system test campaign

  15. Predictability of physiological testing and the role of maturation in talent identification for adolescent team sports.

    Science.gov (United States)

    Pearson, D T; Naughton, G A; Torode, M

    2006-08-01

    Entrepreneurial marketing of sport increases demands on sport development officers to identify talented individuals for specialist development at the youngest possible age. Talent identification results in the streamlining of resources to produce optimal returns from a sports investment. However, the process of talent identification for team sports is complex and success prediction is imperfect. The aim of this review is to describe existing practices in physiological tests used for talent identification in team sports and discuss the impact of maturity-related differences on the long term outcomes particularly for male participants. Maturation is a major confounding variable in talent identification during adolescence. A myriad of hormonal changes during puberty results in physical and physiological characteristics important for sporting performance. Significant changes during puberty make the prediction of adult performance difficult from adolescent data. Furthermore, for talent identification programs to succeed, valid and reliable testing procedures must be accepted and implemented in a range of performance-related categories. Limited success in scientifically based talent identification is evident in a range of team sports. Genetic advances challenge the ethics of talent identification in adolescent sport. However, the environment remains a significant component of success prediction in sport. Considerations for supporting talented young male athletes are discussed.

  16. Study of the feasibility aspects of flight testing an aeroelastically tailored forward swept research wing on a BQM-34F drone vehicle

    Science.gov (United States)

    Mourey, D. J.

    1979-01-01

    The aspects of flight testing an aeroelastically tailored forward swept research wing on a BQM-34F drone vehicle are examined. The geometry of a forward swept wing, which is incorporated into the BQM-34F to maintain satisfactory flight performance, stability, and control is defined. A preliminary design of the aeroelastically tailored forward swept wing is presented.

  17. Design and Analysis of a Cable-Driven Test Apparatus for Flapping-Flight Research

    OpenAIRE

    Musick, Stephen J.

    2014-01-01

    The biology, physiology, kinematics, and aerodynamics of insect flight have been a longstanding fascination for biologists and engineers. The former three are easily obtained through the observation of the organic species. The latter though, is very difficult to study in this fashion. In many cases, aerodynamic forces and fluid-body interactions can be simulated with computational fluid dynamics; another option is to use dynamically-scaled, experimental set-ups to measure physically these val...

  18. Guidance and Control of an Autonomous Soaring Vehicle with Flight Test Results

    Science.gov (United States)

    Allen, Michael J.

    2007-01-01

    A guidance and control method was developed to detect and exploit thermals for energy gain. Latency in energy rate estimation degraded performance. The concept of a UAV harvesting energy from the atmosphere has been shown to be feasible with existing technology. Many UAVs have similar mission constraints to birds and sailplanes. a) Surveillance; b) Point to point flight with minimal energy; and c) Increased ground speed.

  19. Identification of pathogenic microorganisms directly from positive blood vials by matrix-assisted laser desorption/ionization time of flight mass spectrometry

    DEFF Research Database (Denmark)

    Nonnemann, Bettina; Tvede, Michael; Bjarnsholt, Thomas

    2013-01-01

    Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is a promising and fast method for identifying fungi and bacteria directly from positive blood cultures. Various pre-treatment methods for MALDI-TOF MS identification have been reported for this purpose. In......-house results for identification of bacterial colonies by MALDI-TOF MS using a cut-off score of 1.5 did not reduce the diagnostic accuracy compared with the recommended cut-off score of 1.8. A 3-month consecutive study of positive blood cultures was carried out in our laboratory to evaluate whether...... the Sepsityper™ Kit (Bruker Daltonics) with Biotyper 2.0 software could be used as a fast diagnostic tool for bacteria and fungi and whether a 1.5 cut-off score could improve species identification compared with the recommended score of 1.8. Two hundred and fifty-six positive blood vials from 210 patients and 19...

  20. Identification Report: Earthquake Test on 2-Bay, 6-Story Scale 1:5 RC-Frames

    DEFF Research Database (Denmark)

    Kirkegaard, Poul Henning; Skjærbæk, P. S.; Nielsen, Søren R. K.

    The aim of the present report is to supply the identification results from the tests performed with a laboratory model of a plane 6-storey, 2-bay scale 1:5 RC-frame at Aalborg University, Denmark during the autumn 1996. The tests were performed as a part of a Ph.D study considering evaluation of ...

  1. Evaluating Gifted Identification Practice: Aptitude Testing and Linguistically Diverse Learners

    Science.gov (United States)

    Matthews, Michael S.; Kirsch, Lauri

    2011-01-01

    The authors examined individually administered IQ scores from an entire K-5 population (N = 432) of Limited English Proficient students referred for gifted program eligibility determination in a single large urban district in the southeastern United States. Of 8 IQ tests compared, only 1, the Stanford-Binet V, had scores appreciably lower than…

  2. 40 CFR 136.3 - Identification of test procedures.

    Science.gov (United States)

    2010-07-01

    ... availability of this material at NARA, call 202-741-6030, or go to: http://www.archives.gov/federal_register... density of the water sample. 8 When the MF method has been used previously to test waters with high... Wastewater, 15th Edition. 31 For samples known or suspected to contain high levels of silver (e.g., in excess...

  3. Follow on Research for Multi-Utility Technology Test Bed Aircraft at NASA Dryden Flight Research Center (FY13 Progress Report)

    Science.gov (United States)

    Pak, Chan-Gi

    2013-01-01

    Modern aircraft employ a significant fraction of their weight in composite materials to reduce weight and improve performance. Aircraft aeroservoelastic models are typically characterized by significant levels of model parameter uncertainty due to the composite manufacturing process. Small modeling errors in the finite element model will eventually induce errors in the structural flexibility and mass, thus propagating into unpredictable errors in the unsteady aerodynamics and the control law design. One of the primary objectives of Multi Utility Technology Test-bed (MUTT) aircraft is the flight demonstration of active flutter suppression, and therefore in this study, the identification of the primary and secondary modes for the structural model tuning based on the flutter analysis of MUTT aircraft. The ground vibration test-validated structural dynamic finite element model of the MUTT aircraft is created in this study. The structural dynamic finite element model of MUTT aircraft is improved using the in-house Multi-disciplinary Design, Analysis, and Optimization tool. In this study, two different weight configurations of MUTT aircraft have been improved simultaneously in a single model tuning procedure.

  4. Measuring structure deformations of a composite glider by optical means with on-ground and in-flight testing

    Science.gov (United States)

    Bakunowicz, Jerzy; Święch, Łukasz; Meyer, Ralf

    2016-12-01

    In aeronautical research experimental data sets of high quality are essential to verify and improve simulation algorithms. For this reason the experimental techniques need to be constantly refined. The shape, movement or deformation of structural aircraft elements can be measured implicitly in multiple ways; however, only optical, correlation-based techniques are able to deliver direct high-order and spatial results. In this paper two different optical metrologies are used for on-ground preparation and the actual execution of in-flight wing deformation measurements on a PW-6U glider. Firstly, the commercial PONTOS system is used for static tests on the ground and for wind tunnel investigations to successfully certify an experimental sensor pod mounted on top of the test bed fuselage. Secondly, a modification of the glider is necessary to implement the optical method named image pattern correlation technique (IPCT), which has been developed by the German Aerospace Center DLR. This scientific technology uses a stereoscopic camera set-up placed inside the experimental pod and a stochastic dot matrix applied to the area of interest on the glider wing to measure the deformation of the upper wing surface in-flight. The flight test installation, including the preparation, is described and results are presented briefly. Focussing on the compensation for typical error sources, the paper concludes with a recommended procedure to enhance the data processing for better results. Within the presented project IPCT has been developed and optimized for a new type of test bed. Adapted to the special requirements of the glider, the IPCT measurements were able to deliver a valuable wing deformation data base which now can be used to improve corresponding numerical models and simulations.

  5. Flight Reynolds Number Testing of the Orion Launch Abort Vehicle in the NASA Langley National Transonic Facility

    Science.gov (United States)

    Chan, David T.; Brauckmann, Gregory J.

    2011-01-01

    A 6%-scale unpowered model of the Orion Launch Abort Vehicle (LAV) ALAS-11-rev3c configuration was tested in the NASA Langley National Transonic Facility to obtain static aerodynamic data at flight Reynolds numbers. Subsonic and transonic data were obtained for Mach numbers between 0.3 and 0.95 for angles of attack from -4 to +22 degrees and angles of sideslip from -10 to +10 degrees. Data were also obtained at various intermediate Reynolds numbers between 2.5 million and 45 million depending on Mach number in order to examine the effects of Reynolds number on the vehicle. Force and moment data were obtained using a 6-component strain gauge balance that operated both at warm temperatures (+120 . F) and cryogenic temperatures (-250 . F). Surface pressure data were obtained with electronically scanned pressure units housed in heated enclosures designed to survive cryogenic temperatures. Data obtained during the 3-week test entry were used to support development of the LAV aerodynamic database and to support computational fluid dynamics code validation. Furthermore, one of the outcomes of the test was the reduction of database uncertainty on axial force coefficient for the static unpowered LAV. This was accomplished as a result of good data repeatability throughout the test and because of decreased uncertainty on scaling wind tunnel data to flight.

  6. Use Of The Operational Air Quality Monitor (AQM) For In-Flight Water Testing Project

    Science.gov (United States)

    Macatangay, Ariel

    2014-01-01

    A primary requirement for manned spaceflight is Environmental Health which ensures air and water contaminants, acoustic profiles, microbial flora, and radiation exposures within the cabin are maintained to levels needed for crew health and for vehicle system functionality. The reliance on ground analyses of returned samples is a limitation in the current environmental monitoring strategy that will prevent future Exploration missions beyond low-Earth orbit. This proposal attempts to address this shortcoming by advancing in-flight analyses of water and air. Ground analysis of in-flight, air and water samples typically employ vapor-phase analysis by gas chromatography-mass spectrometry (GC-MS) to identify and quantify organic compounds present in the samples. We envision the use of newly-developed direct ionization approaches as the most viable avenue leading towards an integrated analytical platform for the monitoring of water, air, and, potentially bio-samples in the cabin environment. Development of an in-flight instrument capable of analyzing air and water samples would be the logical next step to meeting the environmental monitoring needs of Exploration missions. Currently, the Air Quality Monitor (AQM) on-board ISS provides this specific information for a number of target compounds in the air. However, there is a significant subset of common target compounds between air and water. Naturally, the following question arises, "Can the AQM be used for both air and water quality monitoring?" Previous directorate-level IR&D funding led to the development of a water sample introduction method for mass spectrometry using electrothermal vaporization (ETV). This project will focus on the integration of the ETV with a ground-based AQM. The capabilities of this integrated platform will be evaluated using a subset of toxicologically important compounds.

  7. Non-Halal biomarkers identification based on Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography-Time of Flight Mass Spectroscopy (GC-TOF MS) techniques

    Science.gov (United States)

    Witjaksono, Gunawan; Saputra, Irwan; Latief, Marsad; Jaswir, Irwandi; Akmeliawati, Rini; Abdelkreem Saeed Rabih, Almur

    2017-11-01

    Consumption of meat from halal (lawful) sources is essential for Muslims. The identification of non-halal meat is one of the main issues that face consumers in meat markets, especially in non-Islamic countries. Pig is one of the non-halal sources of meat, and hence pig meat and its derivatives are forbidden for Muslims to consume. Although several studies have been conducted to identify the biomarkers for nonhalal meats like pig meat, these studies are still in their infancy stages, and as a result there is no universal biomarker which could be used for clear cut identification. The purpose of this paper is to use Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography-Time of Flight Mass Spectroscopy (GC-TOF MS) techniques to study fat of pig, cow, lamb and chicken to find possible biomarkers for pig fat (lard) identification. FTIR results showed that lard and chicken fat have unique peaks at wavenumbers 1159.6 cm-1, 1743.4 cm-1, 2853.1 cm-1 and 2922.5 cm-1 compared to lamb and beef fats which did not show peaks at these wavenumbers. On the other hand, GC/MS-TOF results showed that the concentration of 1,2,3-trimethyl-Benzene, Indane, and Undecane in lard are 250, 14.5 and 1.28 times higher than their concentrations in chicken fat, respectively, and 91.4, 2.3 and 1.24 times higher than their concentrations in cow fat, respectively. These initial results clearly indicate that there is a possibility to find biomarkers for non-halal identification.

  8. Cost Savings Realized by Implementation of Routine Microbiological Identification by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.

    Science.gov (United States)

    Tran, Anthony; Alby, Kevin; Kerr, Alan; Jones, Melissa; Gilligan, Peter H

    2015-08-01

    Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is an emerging technology for rapid identification of bacterial and fungal isolates. In comparison to conventional methods, this technology is much less labor intensive and can provide accurate and reliable results in minutes from a single isolated colony. We compared the cost of performing the bioMérieux Vitek MALDI-TOF MS with conventional microbiological methods to determine the amount saved by the laboratory by converting to the new technology. Identification costs for 21,930 isolates collected between April 1, 2013, and March 31, 2014, were directly compared for MALDI-TOF MS and conventional methodologies. These isolates were composed of commonly isolated organisms, including commonly encountered aerobic and facultative bacteria and yeast but excluding anaerobes and filamentous fungi. Mycobacterium tuberculosis complex and rapidly growing mycobacteria were also evaluated for a 5-month period during the study. Reagent costs and a total cost analysis that included technologist time in addition to reagent expenses and maintenance service agreement costs were analyzed as part of this study. The use of MALDI-TOF MS equated to a net savings of $69,108.61, or 87.8%, in reagent costs annually compared to traditional methods. When total costs are calculated to include technologist time and maintenance costs, traditional identification would have cost $142,532.69, versus $68,886.51 with the MALDI-TOF MS method, resulting in a laboratory savings of $73,646.18, or 51.7%, annually by adopting the new technology. The initial cost of the instrument at our usage level would be offset in about 3 years. MALDI-TOF MS not only represents an innovative technology for the rapid and accurate identification of bacterial and fungal isolates, it also provides a significant cost savings for the laboratory. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  9. The development of an airborne information management system for flight test

    Science.gov (United States)

    Bever, Glenn A.

    1992-01-01

    An airborne information management system is being developed at the NASA Dryden Flight Research Facility. This system will improve the state of the art in management data acquisition on-board research aircraft. The design centers around highly distributable, high-speed microprocessors that allow data compression, digital filtering, and real-time analysis. This paper describes the areas of applicability, approach to developing the system, potential for trouble areas, and reasons for this development activity. System architecture (including the salient points of what makes it unique), design philosophy, and tradeoff issues are also discussed.

  10. Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time

    OpenAIRE

    Lagacé-Wiens, Philippe R. S.; Adam, Heather J.; Karlowsky, James A.; Nichol, Kimberly A.; Pang, Paulette F.; Guenther, Jodi; Webb, Amanda A.; Miller, Crystal; Alfa, Michelle J.

    2012-01-01

    Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry represents a revolution in the rapid identification of bacterial and fungal pathogens in the clinical microbiology laboratory. Recently, MALDI-TOF has been applied directly to positive blood culture bottles for the rapid identification of pathogens, leading to reductions in turnaround time and potentially beneficial patient impacts. The development of a commercially available extraction kit (Bruker Sepsit...

  11. An in-house assay is superior to Sepsityper for direct matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry identification of yeast species in blood cultures.

    Science.gov (United States)

    Bidart, Marie; Bonnet, Isabelle; Hennebique, Aurélie; Kherraf, Zine Eddine; Pelloux, Hervé; Berger, François; Cornet, Muriel; Bailly, Sébastien; Maubon, Danièle

    2015-05-01

    We developed an in-house assay for the direct identification, by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, of yeasts in blood culture. Sixty-one representative strains from 12 species were analyzed in spiked blood cultures. Our assay accurately identified 95 of 107 (88.8%) positive blood cultures and outperformed the commercial Sepsityper kit (81.7% identification). Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  12. Identification and quantification of flavonoids and chromes in Baeckea frutescens by using HPLC coupled with diode-array detection and quadruple time-of-flight mass spectrometry.

    Science.gov (United States)

    Jia, Bei-Xi; Huangfu, Qian-Qian; Ren, Feng-Xiao; Jia, Lu; Zhang, Yan-Bing; Liu, Hong-Min; Yang, Jie; Wang, Qiang

    2015-01-01

    This article marks the first report on high-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD) and quadruple time-of-flight mass spectrometry (Q-TOF/MS) for the identification and quantification of main bioactive constituents in Baeckea frutescens. In total, 24 compounds were identified or tentatively characterised based on their retention behaviours, UV profiles and MS fragment information. Furthermore, a validated method with good linearity, sensitivity, precision, stability, repeatability and accuracy was successfully applied for simultaneous determination of five flavonoids and one chromone in different plant parts of B. frutescens collected at different harvest times, and their dynamic contents revealed the appropriate harvest times. The established HPLC-DAD-Q-TOF/MS using multi-bioactive markers was proved to be a validated strategy for the quality evaluation on both raw materials and related products of B. frutescens.

  13. Identification of the Quality Spot Welding used Non Destructive Test-Ultrasonic Testing: (Effect of Welding Time)

    Science.gov (United States)

    Sifa, A.; Endramawan, T.; Badruzzaman

    2017-03-01

    Resistance Spot Welding (RSW) is frequently used as one way of welding is used in the manufacturing process, especially in the automotive industry [4][5][6][7]. Several parameters influence the process of welding points. To determine the quality of a welding job needs to be tested, either by damaging or testing without damage, in this study conducted experimental testing the quality of welding or identify quality of the nugget by using Non-Destructive Test (NDT) -Ultrasonic Testing (UT), in which the identification of the quality of the welding is done with parameter thickness of worksheet after welding using NDT-UT with use same material worksheet and have more thickness of worksheet, the thickness of the worksheet single plate 1mm, with the capability of propagation Ultrasonic Testing (UT) standard limited> 3 mm [1], welding process parameters such as the time difference between 1-10s and the welding current of 8 KV, visually Heat Affected Zone ( HAZ ) have different results due to the length of time of welding. UT uses a probe that is used with a frequency of 4 MHz, diameter 10 mm, range 100 and the couplant used is oil. Identification techniques using drop 6dB, with sound velocity 2267 m / s of Fe, with the result that the effect of the Welding time affect the size of the HAZ, identification with the lowest time 1s show results capable identified joined through NDT - UT.

  14. Low temperature storage test phase 2 : identification of problem species

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-12-15

    The use of renewable fuels such as biodiesel, in motor vehicle fuels is expected to grow rapidly in North America as a result of governmental mandates. Biodiesel is a fuel component made from plant and animal feedstocks via a transesterification process. The fatty acid methyl esters (FAME) of biodiesel have cloud points that range from 5 degrees C to -15 degrees C. The poor low temperature performance of blends containing FAME must be understood in order to avoid operability issues. This paper presented the results of several testing programs conducted by researchers to investigate filter plugging in biodiesel fuels caused by high levels of saturated monoglycerides. The low temperature storage stability of 57 biodiesel fuels comprised of B5 and B20 made with canola methyl ester (CME), soybean methyl ester (SME), tallow methyl ester (TME) and palm methyl ester (PME) was investigated. Filter blocking tests were conducted to assess storage stability. Deposits from the blends were analyzed using gas chromatography and mass spectrometry (GC-MS) in order to identify the problem species. Results of the study confirmed the deleterious impact of saturated mono-glycerides in FAME on the low temperature operability of filters in fuel handling systems. 11 refs., 7 tabs., 5 figs. 9 appendices.

  15. USAF Flight Test Investigation of Focused Sonic Booms: Project Have Bears

    Science.gov (United States)

    Downing, Micah; Zamot, Noel; Moss, Chris; Morin, Daniel; Wolski, Ed; Chung, Sukhwan; Plotkin, Kenneth; Maglieri, Domenic

    1996-01-01

    Supersonic operations from military aircraft generate sonic booms that can affect people, animals and structures. A substantial experimental data base exists on sonic booms for aircraft in steady flight and confidence in the predictive techniques has been established. All the focus sonic boom data that are in existence today were collected during the 60's and 70's as part of the information base to the US Supersonic Transport program and the French Jericho studies for the Concorde. These experiments formed the data base to develop sonic boom propagation and prediction theories for focusing. There is a renewed interest in high-speed transports for civilian application. Moreover, today's fighter aircraft have better performance capabilities, and supersonic flights ars more common during air combat maneuvers. Most of the existing data on focus booms are related to high-speed civil operations such as transitional linear accelerations and mild turns. However, military aircraft operating in training areas perform more drastic maneuvers such as dives and high-g turns. An update and confirmation of USAF prediction capabilities is required to demonstrate the ability to predict and control sonic boom impacts, especially those produced by air combat maneuvers.

  16. Theoretical morphology and development of flight feather vane asymmetry with experimental tests in parrots.

    Science.gov (United States)

    Feo, Teresa J; Prum, Richard O

    2014-06-01

    Asymmetry in flight feather vane width is a major functional innovation associated with the evolution of flight in the ancestors of birds. However, the developmental and morphological basis of feather shape is not simple, and the developmental processes involved in vane width asymmetry are poorly understood. We present a theoretical model of feather morphology and development that describes the possible ways to modify feather development and produce vane asymmetry. Our model finds that the theoretical morphospace of feather shape is redundant, and that many different combinations of parameters could be responsible for vane asymmetry in a given feather. Next, we empirically measured morphological and developmental model parameters in asymmetric and symmetric feathers from two species of parrots to identify which combinations of parameters create vane asymmetry in real feathers. We found that both longer barbs, and larger barb angles in the relatively wider trailing vane drove asymmetry in tail feathers. Developmentally, longer barbs were the result of an offset of the radial position of the new barb locus, whereas larger barb angles were produced by differential expansion of barbs as the feather unfurls from the tubular feather germ. In contrast, the helical angle of barb ridge development did not contribute to vane asymmetry and could be indicative of a constraint. This research provides the first comprehensive description of both the morphological and developmental modifications responsible for vane asymmetry within real feathers, and identifies key steps that must have occurred during the evolution of vane asymmetry. © 2014 Wiley Periodicals, Inc.

  17. Flight Test Results of the Earth Observing-1 Advanced Land Imager Advanced Land Imager

    Science.gov (United States)

    Mendenhall, Jeffrey A.; Lencioni, Donald E.; Hearn, David R.; Digenis, Constantine J.

    2002-09-01

    The Advanced Land Imager (ALI) is the primary instrument on the Earth Observing-1 spacecraft (EO-1) and was developed under NASA's New Millennium Program (NMP). The NMP mission objective is to flight-validate advanced technologies that will enable dramatic improvements in performance, cost, mass, and schedule for future, Landsat-like, Earth Science Enterprise instruments. ALI contains a number of innovative features designed to achieve this objective. These include the basic instrument architecture, which employs a push-broom data collection mode, a wide field-of-view optical design, compact multi-spectral detector arrays, non-cryogenic HgCdTe for the short wave infrared bands, silicon carbide optics, and a multi-level solar calibration technique. The sensor includes detector arrays that operate in ten bands, one panchromatic, six VNIR and three SWIR, spanning the range from 0.433 to 2.35 μm. Launched on November 21, 2000, ALI instrument performance was monitored during its first year on orbit using data collected during solar, lunar, stellar, and earth observations. This paper will provide an overview of EO-1 mission activities during this period. Additionally, the on-orbit spatial and radiometric performance of the instrument will be compared to pre-flight measurements and the temporal stability of ALI will be presented.

  18. AirSTAR: A UAV Platform for Flight Dynamics and Control System Testing

    Science.gov (United States)

    Jordan, Thomas L.; Foster, John V.; Bailey, Roger M.; Belcastro, Christine M.

    2006-01-01

    As part of the NASA Aviation Safety Program at Langley Research Center, a dynamically scaled unmanned aerial vehicle (UAV) and associated ground based control system are being developed to investigate dynamics modeling and control of large transport vehicles in upset conditions. The UAV is a 5.5% (seven foot wingspan), twin turbine, generic transport aircraft with a sophisticated instrumentation and telemetry package. A ground based, real-time control system is located inside an operations vehicle for the research pilot and associated support personnel. The telemetry system supports over 70 channels of data plus video for the downlink and 30 channels for the control uplink. Data rates are in excess of 200 Hz. Dynamic scaling of the UAV, which includes dimensional, weight, inertial, actuation, and control system scaling, is required so that the sub-scale vehicle will realistically simulate the flight characteristics of the full-scale aircraft. This testbed will be utilized to validate modeling methods, flight dynamics characteristics, and control system designs for large transport aircraft, with the end goal being the development of technologies to reduce the fatal accident rate due to loss-of-control.

  19. The geometry of distributional preferences and a non-parametric identification approach: The Equality Equivalence Test.

    Science.gov (United States)

    Kerschbamer, Rudolf

    2015-05-01

    This paper proposes a geometric delineation of distributional preference types and a non-parametric approach for their identification in a two-person context. It starts with a small set of assumptions on preferences and shows that this set (i) naturally results in a taxonomy of distributional archetypes that nests all empirically relevant types considered in previous work; and (ii) gives rise to a clean experimental identification procedure - the Equality Equivalence Test - that discriminates between archetypes according to core features of preferences rather than properties of specific modeling variants. As a by-product the test yields a two-dimensional index of preference intensity.

  20. Identification of clinical isolates of Aspergillus, including cryptic species, by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

    Science.gov (United States)

    Vidal-Acuña, M Reyes; Ruiz-Pérez de Pipaón, Maite; Torres-Sánchez, María José; Aznar, Javier

    2017-12-08

    An expanded library of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been constructed using the spectra generated from 42 clinical isolates and 11 reference strains, including 23 different species from 8 sections (16 cryptic plus 7 noncryptic species). Out of a total of 379 strains of Aspergillus isolated from clinical samples, 179 strains were selected to be identified by sequencing of beta-tubulin or calmodulin genes. Protein spectra of 53 strains, cultured in liquid medium, were used to construct an in-house reference database in the MALDI-TOF MS. One hundred ninety strains (179 clinical isolates previously identified by sequencing and the 11 reference strains), cultured on solid medium, were blindy analyzed by the MALDI-TOF MS technology to validate the generated in-house reference database. A 100% correlation was obtained with both identification methods, gene sequencing and MALDI-TOF MS, and no discordant identification was obtained. The HUVR database provided species level (score of ≥2.0) identification in 165 isolates (86.84%) and for the remaining 25 (13.16%) a genus level identification (score between 1.7 and 2.0) was obtained. The routine MALDI-TOF MS analysis with the new database, was then challenged with 200 Aspergillus clinical isolates grown on solid medium in a prospective evaluation. A species identification was obtained in 191 strains (95.5%), and only nine strains (4.5%) could not be identified at the species level. Among the 200 strains, A. tubingensis was the only cryptic species identified. We demonstrated the feasibility and usefulness of the new HUVR database in MALDI-TOF MS by the use of a standardized procedure for the identification of Aspergillus clinical isolates, including cryptic species, grown either on solid or liquid media. © The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For

  1. Reuse fo a Cold War Surveillance Drone to Flight Test a NASA Rocket Based Combined Cycle Engine

    Science.gov (United States)

    Brown, T. M.; Smith, Norm

    1999-01-01

    Plans for and early feasibility investigations into the modification of a Lockheed D21B drone to flight test the DRACO Rocket Based Combined Cycle (RBCC) engine are discussed. Modifications include the addition of oxidizer tanks, modern avionics systems, actuators, and a vehicle recovery system. Current study results indicate that the D21B is a suitable candidate for this application and will allow demonstrations of all DRACO engine operating modes at Mach numbers between 0.8 and 4.0. Higher Mach numbers may be achieved with more extensive modification. Possible project risks include low speed stability and control, and recovery techniques.

  2. Atmospheric pressure gas chromatography coupled to quadrupole-time of flight mass spectrometry as a powerful tool for identification of non intentionally added substances in acrylic adhesives used in food packaging materials.

    Science.gov (United States)

    Canellas, E; Vera, P; Domeño, C; Alfaro, P; Nerín, C

    2012-04-27

    Acrylic adhesives are used to manufacture multilayer laminates that are used in food packaging to form the geometric shape of the package as well as to stick labels on the packages. Once applied on the packaging adhesives can supply potential migrants that could endanger the packaged food. Adhesives are complex matrices where intentionally and non intentionally added substances are present, but the identification of the migrants is required by law. In this study atmospheric pressure gas chromatography coupled to a quadrupole hyphenated to a time of flight mass spectrometer (APGC-MS/Q-TOF) has been explored for identification of unknowns coming from three different acrylic adhesives. The results are compared to those obtained by conventional GC-MS-Q (quadrupole). Sixteen compounds were identified by GC-MS/Q and five of them were confirmed by APGC-MS/Q-TOF as their molecular ions were found. Moreover, additional three new compounds were identified and their structure was elucidated working with the spectra obtained by APGC-MS/Q-TOF. This finding was very relevant as these compounds were biocides suspected to be allergenic and cytotoxic in humans. Migration studies were carried out using Tenax as solid food simulant and the results showed that the three acrylic adhesives tested in this work were safe for being used in food packaging materials since the migration of compounds previously identified was below the limit established in the current legislation. Copyright © 2012 Elsevier B.V. All rights reserved.

  3. A statistical test for outlier identification in data envelopment analysis

    Directory of Open Access Journals (Sweden)

    Morteza Khodabin

    2010-09-01

    Full Text Available In the use of peer group data to assess individual, typical or best practice performance, the effective detection of outliers is critical for achieving useful results. In these ‘‘deterministic’’ frontier models, statistical theory is now mostly available. This paper deals with the statistical pared sample method and its capability of detecting outliers in data envelopment analysis. In the presented method, each observation is deleted from the sample once and the resulting linear program is solved, leading to a distribution of efficiency estimates. Based on the achieved distribution, a pared test is designed to identify the potential outlier(s. We illustrate the method through a real data set. The method could be used in a first step, as an exploratory data analysis, before using any frontier estimation.

  4. X-43A Flight Controls

    Science.gov (United States)

    Baumann, Ethan

    2006-01-01

    A viewgraph presentation detailing X-43A Flight controls at NASA Dryden Flight Research Center is shown. The topics include: 1) NASA Dryden, Overview and current and recent flight test programs; 2) Unmanned Aerial Vehicle Synthetic Aperture Radar (UAVSAR) Program, Program Overview and Platform Precision Autopilot; and 3) Hyper-X Program, Program Overview, X-43A Flight Controls and Flight Results.

  5. [Evaluation of a rapid trehalase test for the identification of Candida glabrata].

    Science.gov (United States)

    Kirdar, Sevin; Gültekin, Berna; Evcil, Gonca; Ozkütük, Aydan; Sener, Asli Gamze; Aydin, Neriman

    2009-04-01

    Candida species which cause local infections, may also lead to fatal systemic infections. The increasing incidence of non-albicans Candida, especially fluconazole susceptible or resistant dose-dependent C. glabrata, increased the importance of rapid and accurate species level identification for Candida. Rapid and correct identification of C. glabrata is essential for the initiation of the appropriate antifungal therapy. This study was conducted to evaluate the performance of the rapid trehalase test in the diagnosis of C. glabrata isolates. A total of 173 Candida strains isolated from various clinical specimens and identified according to germ tube test, growth on cornmeal Tween 80 agar and the colony morphologies on Mast-CHROMagar Candida medium (Mast Diagnostics, UK), were included to the study. The identification of non-albicans Candida species were also confirmed by API 20CAUX (BioMerieux, France) system. Accordingly 86 (50%) of the isolates were identified as C. glabrata, 48 (28%) C. albicans, 17 (10%) C. krusei, 13 (8%) C. tropicalis, 5 (3%) C. parapsilosis, 3 (2%) C. kefyr and 1 (1%) Cutilis. In order to detect the presence of trehalase enzyme in Condida strains, all isolates were grown on Sabouraud dextrose agar containing 4% glucose and then one yeast colony was emulsified in 50 microl of citrate buffer containing 4% (wt/vol) trehalose for 3 h at 37 degrees C. Presence of glucose which emerged after the action of trehalase on trehalose, was detected by a commercial "urinary glucose detection dipstick" (Spinreacta, Spain). All C. glabrata strains yielded positive result by trehalase test. None C. glabrata isolates were found negative by trehalase test except for one strain of C. tropicalis. In this study, the trehalase test allowed identification of C. globrata with 100% sensitivity and 98.9% specificity. It was concluded that trehalase test is a rapid, cost-effective and simple test that can be used for the accurate identification of C. glabrata.

  6. Results from Navigator GPS Flight Testing for the Magnetospheric MultiScale Mission

    Science.gov (United States)

    Lulich, Tyler D.; Bamford, William A.; Wintermitz, Luke M. B.; Price, Samuel R.

    2012-01-01

    The recent delivery of the first Goddard Space Flight Center (GSFC) Navigator Global Positioning System (GPS) receivers to the Magnetospheric MultiScale (MMS) mission spacecraft is a high water mark crowning a decade of research and development in high-altitude space-based GPS. Preceding MMS delivery, the engineering team had developed receivers to support multiple missions and mission studies, such as Low Earth Orbit (LEO) navigation for the Global Precipitation Mission (GPM), above the constellation navigation for the Geostationary Operational Environmental Satellite (GOES) proof-of-concept studies, cis-Lunar navigation with rapid re-acquisition during re-entry for the Orion Project and an orbital demonstration on the Space Shuttle during the Hubble Servicing Mission (HSM-4).

  7. Isotope Brayton ground demonstration testing and flight qualification. Volume 1. Technical program

    Energy Technology Data Exchange (ETDEWEB)

    1974-12-09

    A program is proposed for the ground demonstration, development, and flight qualification of a radioisotope nuclear heated dynamic power system for use on space missions beginning in the 1980's. This type of electrical power system is based upon and combines two aerospace technologies currently under intense development; namely, the MHW isotope heat source and the closed Brayton cycle gas turbine. This power system represents the next generation of reliable, efficient economic electrical power equipment for space, and will be capable of providing 0.5 to 2.0 kW of electric power to a wide variety of spacecraft for earth orbital and interplanetary missions. The immediate design will be based upon the requirements for the Air Force SURVSATCOM mission. The proposal is presented in three volumes plus an Executive Summary. This volume describes the tasks in the technical program.

  8. Comparison of Numerical Analyses with a Static Load Test of a Continuous Flight Auger Pile

    Directory of Open Access Journals (Sweden)

    Hoľko Michal

    2014-12-01

    Full Text Available The article deals with numerical analyses of a Continuous Flight Auger (CFA pile. The analyses include a comparison of calculated and measured load-settlement curves as well as a comparison of the load distribution over a pile's length. The numerical analyses were executed using two types of software, i.e., Ansys and Plaxis, which are based on FEM calculations. Both types of software are different from each other in the way they create numerical models, model the interface between the pile and soil, and use constitutive material models. The analyses have been prepared in the form of a parametric study, where the method of modelling the interface and the material models of the soil are compared and analysed.

  9. Orion Heat Shield Manufacturing Producibility Improvements for the EM-1 Flight Test Program

    Science.gov (United States)

    Koenig, William J.; Stewart, Michael; Harris, Richard F.

    2018-01-01

    This paper describes how the ORION program is incorporating improvements in the heat shield design and manufacturing processes reducing programmatic risk and ensuring crew safety in support of NASA's Exploration missions. The approach for the EFT-1 heat shield utilized a low risk Apollo heritage design and manufacturing process using an Avcoat TPS ablator with a honeycomb substrate to provide a one piece heat shield to meet the mission re-entry heating environments. The EM-1 mission will have additional flight systems installed to fly to the moon and return to Earth. Heat shield design and producibility improvements have been incorporated in the EM-1 vehicle to meet deep space mission requirements. The design continues to use the Avcoat material, but in a block configuration to enable improvements in consistant and repeatable application processes using tile bonding experience developed on the Space Shuttle Transportation System Program.

  10. Boundary Layer Transition During the Orion Exploration Flight Test 1 (EFT-1)

    Science.gov (United States)

    Kirk, Lindsay C.

    2016-01-01

    Boundary layer transition was observed in the thermocouple data on the windside backshell of the Orion reentry capsule. Sensors along the windside centerline, as well as off-centerline, indicated transition late in the flight at approximately Mach 4 conditions. Transition progressed as expected, beginning at the sensors closest to the forward bay cover (FBC) and moving towards the heatshield. Sensors placed in off-centerline locations did not follow streamlines, so the progression of transition observed in these sensors is less intuitive. Future analysis will include comparisons to pre-flight predictions and expected transitional behavior will be investigated. Sensors located within the centerline and off-centerline launch abort system (LAS) attach well cavities on the FBC also showed indications of boundary layer transition. The transition within the centerline cavity was observed in the temperature traces prior to transition onset on the sensors upstream of the cavity. Transition behavior within the off centerline LAS attach well cavity will also be investigated. Heatshield thermocouples were placed within Avcoat plugs to attempt to capture transitional behavior as well as better understand the aerothermal environments. Thermocouples were placed in stacks of two or five vertically within the plugs, but the temperature data obtained at the sensors closest to the surface did not immediately indicate transitional behavior. Efforts to use the in depth thermocouple temperatures to reconstruct the surface heat flux are ongoing and any results showing the onset of boundary layer transition obtained from those reconstructions will also be included in this paper. Transition on additional features of interest, including compression pad ramps, will be included if it becomes available.

  11. Airborne testing and demonstration of a new flight system based on an Aerodyne N2O-CO2-CO-H2O mini-spectrometer

    Science.gov (United States)

    Gvakharia, A.; Kort, E. A.; Smith, M. L.; Conley, S.

    2017-12-01

    Nitrous oxide (N2O) is a powerful greenhouse gas and ozone depleting substance. With high atmospheric backgrounds and small relative signals, N2O emissions have been challenging to observe and understand on regional scales with traditional instrumentation. Fast-response airborne measurements with high precision and accuracy can potentially bridge this observational gap. Here we present flight assessments of a new flight system based on an Aerodyne mini-spectrometer as well as a Los Gatos N2O/CO analyzer during the Fertilizer Emissions Airborne Study (FEAST). With the Scientific Aviation Mooney aircraft, we conducted test flights for both analyzers where a known calibration gas was sampled throughout the flight (`null' tests). Clear altitude/cabin-pressure dependencies were observed for both analyzers if operated in an "off-the-shelf' manner. For the remainder of test flights and the FEAST campaign we used a new flight system based on an Aerodyne mini-spectrometer with the addition of a custom pressure control/calibration system. Instead of using traditional approaches with spectral-zeros and infrequent in-flight calibrations, we employ a high-flow system with stable flow control to enable high frequency (2 minutes), short duration (15 seconds) sampling of a known calibration gas. This approach, supported by the null test, enables correction for spectral drift caused by a variety of factors while maintaining a 90% duty cycle for 1Hz sampling from an aircraft. Preliminary in-flight precisions are estimated at 0.05 ppb, 0.1 ppm, 1 ppb, and 10 ppm for N2O, CO2, CO, and H2O respectively. We also present a further 40 hours of inter-comparison in flight with a Picarro 2301-f ring-down spectrometer demonstrating consistency between CO2 and H2O measurements and no altitude dependent error.

  12. Proteogenomic biomarkers for identification of Francisella species and subspecies by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.

    Science.gov (United States)

    Durighello, Emie; Bellanger, Laurent; Ezan, Eric; Armengaud, Jean

    2014-10-07

    Francisella tularensis is the causative agent of tularemia. Because some Francisella strains are very virulent, this species is considered by the Centers for Disease Control and Prevention to be a potential category A bioweapon. A mass spectrometry method to quickly and robustly distinguish between virulent and nonvirulent Francisella strains is desirable. A combination of shotgun proteomics and whole-cell matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry on the Francisella tularensis subsp. holarctica LVS defined three protein biomarkers that allow such discrimination: the histone-like protein HU form B, the 10 kDa chaperonin Cpn10, and the 50S ribosomal protein L24. We established that their combined detection by whole-cell MALDI-TOF spectrum could enable (i) the identification of Francisella species, and (ii) the prediction of their virulence level, i.e., gain of a taxonomical level with the identification of Francisella tularensis subspecies. The detection of these biomarkers by MALDI-TOF mass spectrometry is straightforward because of their abundance and the absence of other abundant protein species closely related in terms of m/z. The predicted molecular weights for the three biomarkers and their presence as intense peaks were confirmed with MALDI-TOF/MS spectra acquired on Francisella philomiragia ATCC 25015 and on Francisella tularensis subsp. tularensis CCUG 2112, the most virulent Francisella subspecies.

  13. Identification of AKB-48 and 5F-AKB-48 Metabolites in Authentic Human Urine Samples Using Human Liver Microsomes and Time of Flight Mass Spectrometry.

    Science.gov (United States)

    Vikingsson, Svante; Josefsson, Martin; Gréen, Henrik

    2015-01-01

    The occurrence of structurally related synthetic cannabinoids makes the identification of unique markers of drug intake particularly challenging. The aim of this study was to identify unique and abundant metabolites of AKB-48 and 5F-AKB-48 for toxicological screening in urine. Investigations of authentic urine samples from forensic cases in combination with human liver microsome (HLM) experiments were used for identification of metabolites. HLM incubations of AKB-48 and 5F-AKB-48 along with 35 urine samples from authentic cases were analyzed with liquid chromatography quadrupole tandem time of flight mass spectrometry. Using HLMs 41 metabolites of AKB-48 and 37 metabolites of 5F-AKB-48 were identified, principally represented by hydroxylation but also ketone formation and dealkylation. Monohydroxylated metabolites were replaced by di- and trihydroxylated metabolites within 30 min. The metabolites from the HLM incubations accounted for on average 84% (range, 67-100) and 91% (range, 71-100) of the combined area in the case samples for AKB-48 and 5F-AKB-48, respectively. While defluorinated metabolites accounted for on average 74% of the combined area after a 5F-AKB-48 intake only a few identified metabolites were shared between AKB-48 and 5F-AKB-48, illustrating the need for a systematic approach to identify unique metabolites. HLMs in combination with case samples seem suitable for this purpose. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  14. Rapid Identification of Steroidal Saponins in Trillium tschonoskii Maxim by Ultraperformance Liquid Chromatography Coupled to Electrospray Ionisation Quadrupole Time-of-Flight Tandem Mass Spectrometry.

    Science.gov (United States)

    Gao, Xin; Sun, Wenjun; Fu, Qiang; Niu, Xiaofeng

    2015-01-01

    Steroidal saponins in Trillium tschonoskii Maxim have many biological activities, including immunological regulation and anti-tumour. Comprehensive ingredient identification is critical for understanding its pharmacological mechanism and establishing quality control protocols. However, it is a challenging problem because of the complexity of steroidal saponins. To develop a UPLC-MS method for identifying and characterising steroidal saponins in the root and rhizome of T. tschonoskii. Methanolic extracts of T. tschonoskii were analysed by using ultraperformance liquid chromatography coupled to electrospray ionisation quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI/QTOF/MS). The UPLC experiments were performed by means of a reversed-phase C18 -column and a binary mobile phase system consisting of water and acetonitrile with formic acid under gradient elution conditions. For the UPLC-MS measurements, positive and negative ion modes were used in order to obtain better tandem mass spectra and high-resolution mass spectra. Based on retention times, accurate mass and mass spectrometric fragmentation, a total of 31 saponins distributed over eight steroidal aglycone skeletons were identified or tentatively elucidated from T. tschonoskii. The UPLC-ESI/QTOF/MS method has proven to be a powerful tool for rapid identification of steroidal saponins in T. tschonoskii without tedious and time-consuming isolation of pure constituents. Copyright © 2015 John Wiley & Sons, Ltd.

  15. Rapid identification of pathogens directly from blood culture bottles by Bruker matrix-assisted laser desorption laser ionization-time of flight mass spectrometry versus routine methods.

    Science.gov (United States)

    Jamal, Wafaa; Saleem, Rola; Rotimi, Vincent O

    2013-08-01

    The use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of microorganisms directly from blood culture is an exciting dimension to the microbiologists. We evaluated the performance of Bruker SepsiTyper kit™ (STK) for direct identification of bacteria from positive blood culture. This was done in parallel with conventional methods. Nonrepetitive positive blood cultures from 160 consecutive patients were prospectively evaluated by both methods. Of 160 positive blood cultures, the STK identified 114 (75.6%) isolates and routine conventional method 150 (93%). Thirty-six isolates were misidentified or not identified by the kit. Of these, 5 had score of >2.000 and 31 had an unreliable low score of <1.7. Four of 8 yeasts were identified correctly. The average turnaround time using the STK was 35 min, including extraction steps and 30:12 to 36:12 h with routine method. The STK holds promise for timely management of bacteremic patients. Copyright © 2013 Elsevier Inc. All rights reserved.

  16. Applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Ashizawa, Kazuho; Murata, Syota; Terada, Takashi; Ito, Daisuke; Bunya, Masaru; Watanabe, Koji; Teruuchi, Yoko; Tsuchida, Sachio; Satoh, Mamoru; Nishimura, Motoi; Matsushita, Kazuyuki; Sugama, Yuji; Nomura, Fumio

    2017-08-01

    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be used to identify pathogens in blood culture samples. However, sample pretreatment is needed for direct identification of microbes in blood culture bottles. Conventional protocols are complex and time-consuming. Therefore, in this study, we developed a method for collecting bacteria using polyallylamine-polystyrene copolymer for application in wastewater treatment technology. Using representative bacterial species Escherichia coli and Staphylococcus capitis, we found that polyallylamine-polystyrene can form visible aggregates with bacteria, which can be identified using MALDI-TOF MS. The processing time of our protocol was as short as 15min. Hemoglobin interference in MALDI spectra analysis was significantly decreased in our method compared with the conventional method. In a preliminary experiment, we evaluated the use of our protocol to identify clinical isolates from blood culture bottles. MALDI-TOF MS-based identification of 17 strains from five bacterial species (E. coli, Klebsiella pneumoniae, Enterococcus faecalis, S. aureus, and S. capitis) collected by our protocol was satisfactory. Prospective large-scale studies are needed to further evaluate the clinical application of this novel and simple method of collecting bacteria in blood culture bottles. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Development of a PCR test for identification of Haemophilus somnus in pure and mixed cultures

    DEFF Research Database (Denmark)

    Angen, Øystein; Ahrens, Peter; Tegtmeier, Conny

    1998-01-01

    a single colony of H. somnus in the presence of 10(9) CFU of P. multocida even after 2 days of incubation. In conclusion, the present PCR test has been shown to represent a specific test for identification of H. somnus both in pure and mixed cultures. It represents a quick, sensitive and reliable method...... rise to an amplicon in the PCR test. The performance of the test on mixed cultures was evaluated by adding P. multocida to serial dilutions of H. somnus and incubating the agarplates for 1 and 2 days. This showed that the PCR test applied to the harvest from an agarplate can be expected to detect...

  18. Flight Test of a Propulsion-Based Emergency Control System on the MD-11 Airplane with Emphasis on the Lateral Axis

    Science.gov (United States)

    Burken, John J.; Burcham, Frank W., Jr.; Maine, Trindel A.; Feather, John; Goldthorpe, Steven; Kahler, Jeffrey A.

    1996-01-01

    A large, civilian, multi-engine transport MD-11 airplane control system was recently modified to perform as an emergency backup controller using engine thrust only. The emergency backup system, referred to as the propulsion-controlled aircraft (PCA) system, would be used if a major primary flight control system fails. To allow for longitudinal and lateral-directional control, the PCA system requires at least two engines and is implemented through software modifications. A flight-test program was conducted to evaluate the PCA system high-altitude flying characteristics and to demonstrate its capacity to perform safe landings. The cruise flight conditions, several low approaches and one landing without any aerodynamic flight control surface movement, were demonstrated. This paper presents results that show satisfactory performance of the PCA system in the longitudinal axis. Test results indicate that the lateral-directional axis of the system performed well at high attitude but was sluggish and prone to thermal upsets during landing approaches. Flight-test experiences and test techniques are also discussed with emphasis on the lateral-directional axis because of the difficulties encountered in flight test.

  19. Maneuver Acoustic Flight Test of the Bell 430 Helicopter Data Report

    Science.gov (United States)

    Watts, Michael E.; Greenwood, Eric; Smith, Charles D.; Snider, Royce; Conner, David A.

    2014-01-01

    A cooperative ight test by NASA, Bell Helicopter and the U.S. Army to characterize the steady state acoustics and measure the maneuver noise of a Bell Helicopter 430 aircraft was accomplished. The test occurred during June/July 2011 at Eglin Air Force Base, Florida. This test gathered a total of 410 test points over 10 test days and compiled an extensive database of dynamic maneuver measurements. Three microphone arrays with up to 31 microphon. es in each were used to acquire acoustic data. Aircraft data included Differential Global Positioning System, aircraft state and rotor state information. This paper provides an overview of the test and documents the data acquired.

  20. Design and flight test results of high speed optical bidirectional link between stratospheric platforms for aerospace applications

    Science.gov (United States)

    Briatore, S.; Akhtyamov, R.; Golkar, A.

    2017-08-01

    As small and nanosatellites become increasingly relevant in the aerospace industry1, 2, the need of efficient, lightweight and cost-effective networking solutions drives the need for the development of lightweight and low cost networking and communication terminals. In this paper we propose the design and prototype results of a hybrid optical and radio communication architecture developed to fit the coarse pointing capabilities of nanosatellites, tested through a proxy flight experiment on stratospheric balloons. This system takes advantage of the higher data-rate offered by optical communication channels while relying on the more mature and stable technology of conventional radio systems for link negotiation and low-speed data exchange. Such architecture allows the user to overcome the licensing requirements and scarce availability of high data-rate radio frequency channels in the commonly used bands. Outlined are the architecture, development and test of the mentioned terminal, with focus on the communication part and supporting technologies, including the navigation algorithm, the developed fail-safe approach, and the evolution of the pointing system continuing previous work done in 3. The system has been built with commercial-off-the-shelf components and demonstrated on a stratospheric balloon launch campaign. The paper outlines the results of an in-flight demonstration, where the two platforms successfully established an optical link at stratospheric altitudes. The results are then analyzed and contextualized in plans of future work for nanosatellite implementations.