WorldWideScience

Sample records for fish tagging system

  1. Study to Determine the Biological Feasibility of a New Fish Tagging System : Annual Report 1983.

    Energy Technology Data Exchange (ETDEWEB)

    Prentice, Earl F.; Park, D.L.

    1984-05-01

    Pacific salmon are tagged or marked as a critical part of numerous research and management studies. A new tag called the PIT (passive integrated transponder) tag measuring 7.5 mm long by 1.5 mm in diameter has a great potential for marking fish if it proves to be biologically compatible. A study was conducted to evaluate the potential of the PIT tag for marking salmonids. The objectives of the first year's research were to determine: (1) the anatomical areas in which the tag could be placed; (2) tissue response to the tag; and (3) tag retention. Juvenile coho, Oncorhynchus kisutch, and chinook O. tshawytscha, salmon and adult chinook salmon held at Manchester or Big Beef Creek, Washington, were used as test animals. Juvenile salmon were injected with sham PIT tags in the body cavity and opercular, dorsal, and caudal masculature. The fish ranged in length from 126 to 212 mm. Observations based on three tests, from 44 to 102 days long, indicated that the dorsal musculature and body cavity were the best locations to inject the tag from biological and social standpoints. Sham PIT tags were injected into the nose; body cavity; and opercular, dorsal, and caudal musculature of jack chinook salmon. The test was conducted for 23 days. Although all five anatomical areas were acceptable from a technical standpoint, the body cavity appeared to be the best area for tag placement. Initial test results with the Sham PIT tag were very encouraging. Apparently the PIT tag can be successfully injected into and carried by salmon, making it a potentially useful tool for fisheries biologists. 5 refs., 8 figs., 6 tabs.

  2. Discharge residence of TLD tagged fish

    International Nuclear Information System (INIS)

    Romberg, G.P.; Prepejchal, W.

    1974-01-01

    Although visual observations suggested that fish remained in the discharge for considerable periods, temperature-sensitive tags indicated the majority of fish spend less than 50 hr or 10 percent of the time at discharge temperatures. During 1974 a second fish tagging study was conducted, using temperature-sensitive tags to yield discharge residence times of Lake Michigan salmonids at Point Beach thermal discharge. Preliminary results revealed that many fish tag values were close to Unit I line indicating that calculated maximum discharge residence times for these fish will be nearly 100 percent of the elapsed time

  3. The use of external electronic tags on fish: an evaluation of tag retention and tagging effects

    DEFF Research Database (Denmark)

    Jepsen, Niels; Thorstad, Eva B.; Havn, Torgeir

    2015-01-01

    External tagging of fish with electronic tags has been used for decades for a wide range of marine and freshwater species. In the early years of fish telemetry research, it was the most commonly used attachment method, but later internal implants became preferred. Recently, the number of telemetry...... unsuitable for surgical implantation, or when using tags with sensors recording the external environment. The most commonly reported problems with external tags are tissue damage, premature tag loss, and decreased swimming capacity, but the effects are highly context dependent and species specific. Reduced......, but particularly there are few studies on predation risk, social interactions, and studies distinguishing capture and handling effects from tagging effects. For PSATs, especially those that are large relative to fish size, there are particular problems with a high proportion of premature tag losses, reduced...

  4. Raft and floating radio frequency identification (RFID) antenna systems for detecting and estimating abundance of PIT-tagged fish in rivers

    Science.gov (United States)

    Fetherman, Eric R.; Avila, Brian W.; Winkelman, Dana L.

    2016-01-01

    Portable radio frequency identification (RFID) PIT tag antenna systems are increasingly being used in studies examining aquatic animal movement, survival, and habitat use, and their design flexibility permits application in a wide variety of settings. We describe the construction, use, and performance of two portable floating RFID PIT tag antenna systems designed to detect fish that were unavailable for recapture using stationary antennas or electrofishing. A raft antenna system was designed to detect and locate PIT-tagged fish in relatively long (i.e., ≥10 km) river reaches, and consisted of two antennas: (1) a horizontal antenna (4 × 1.2 m) installed on the bottom of the raft and used to detect fish in shallower river reaches (<1 m), and (2) a vertical antenna (2.7 × 1.2 m) for detecting fish in deeper pools (≥1 m). Detection distances of the horizontal antenna were between 0.7 and 1.0 m, and detection probability was 0.32 ± 0.02 (mean ± SE) in a field test using rocks marked with 32-mm PIT tags. Detection probability of PIT-tagged fish in the Cache la Poudre River, Colorado, using the raft antenna system, which covered 21% of the wetted area, was 0.14 ± 0.14. A shore-deployed floating antenna (14.6 × 0.6 m), which covered 100% of the wetted area, was designed for use by two operators for detecting and locating PIT-tagged fish in shorter (i.e., <2 km) river reaches. Detection distances of the shore-deployed floating antenna were between 0.7 and 0.8 m, and detection probabilities during field deployment in the St. Vrain River exceeded 0.52. The shore-deployed floating antenna was also used to estimate abundance of PIT-tagged fish. Results suggest that the shore-deployed floating antenna could be used as an alternative to estimating abundance using traditional sampling methods such as electrofishing.

  5. Raft and floating radio frequency identification (RFID) antenna systems for detecting and estimating abundance of PIT-tagged fish in rivers

    Science.gov (United States)

    Fetherman, Eric R.; Avila, Brian W.; Winkelman, Dana L.

    2016-01-01

    Portable radio frequency identification (RFID) PIT tag antenna systems are increasingly being used in studies examining aquatic animal movement, survival, and habitat use, and their design flexibility permits application in a wide variety of settings. We describe the construction, use, and performance of two portable floating RFID PIT tag antenna systems designed to detect fish that were unavailable for recapture using stationary antennas or electrofishing. A raft antenna system was designed to detect and locate PIT-tagged fish in relatively long (i.e., ≥10 km) river reaches, and consisted of two antennas: (1) a horizontal antenna (4 × 1.2 m) installed on the bottom of the raft and used to detect fish in shallower river reaches (raft antenna system, which covered 21% of the wetted area, was 0.14 ± 0.14. A shore-deployed floating antenna (14.6 × 0.6 m), which covered 100% of the wetted area, was designed for use by two operators for detecting and locating PIT-tagged fish in shorter (i.e., <2 km) river reaches. Detection distances of the shore-deployed floating antenna were between 0.7 and 0.8 m, and detection probabilities during field deployment in the St. Vrain River exceeded 0.52. The shore-deployed floating antenna was also used to estimate abundance of PIT-tagged fish. Results suggest that the shore-deployed floating antenna could be used as an alternative to estimating abundance using traditional sampling methods such as electrofishing.

  6. A Study to Determine the Biological Feasibility of a New Fish Tagging System : Annual Report 1990-1993.

    Energy Technology Data Exchange (ETDEWEB)

    Prentice, Earl F.; Maynard, D.J.; Downing, S.L. (and others)

    1994-01-01

    In 1983, the National Marine Fisheries Service (NMFS) began a multiyear cooperative research program with the Bonneville Power Administration (BPA) to evaluate a new miniaturized identification system that could be used with salmonids. The system is referred to as the passive-integrated-transponder (PIT) tagging and interrogation system. The program has focused on determining the effects of PIT tags on juvenile and adult salmonids, as well as the development and evaluation of tagging and interrogation methods. Earlier results of the program have been reported in annual reports and journal articles cited in this report. This report covers the work per formed from 1990 through 1993. For convenience, the report is divided into three sections: (1) Interrogation and separation systems at Columbia River Basin dams; (2) Systems development and evaluation; and (3) Information and technology transfer.

  7. Personalization of tagging systems

    NARCIS (Netherlands)

    J. Wang (Jun); M. Clements (Maarten); J. Yang; A.P. de Vries (Arjen); M.J.T. Reinders

    2010-01-01

    htmlabstractSocial media systems have encouraged end user participation in the Internet, for the purpose of storing and distributing Internet content, sharing opinions and maintaining relationships. Collaborative tagging allows users to annotate the resulting user-generated content, and enables

  8. A combined telemetry - tag return approach to estimate fishing and natural mortality rates of an estuarine fish

    Science.gov (United States)

    Bacheler, N.M.; Buckel, J.A.; Hightower, J.E.; Paramore, L.M.; Pollock, K.H.

    2009-01-01

    A joint analysis of tag return and telemetry data should improve estimates of mortality rates for exploited fishes; however, the combined approach has thus far only been tested in terrestrial systems. We tagged subadult red drum (Sciaenops ocellatus) with conventional tags and ultrasonic transmitters over 3 years in coastal North Carolina, USA, to test the efficacy of the combined telemetry - tag return approach. There was a strong seasonal pattern to monthly fishing mortality rate (F) estimates from both conventional and telemetry tags; highest F values occurred in fall months and lowest levels occurred during winter. Although monthly F values were similar in pattern and magnitude between conventional tagging and telemetry, information on F in the combined model came primarily from conventional tags. The estimated natural mortality rate (M) in the combined model was low (estimated annual rate ?? standard error: 0.04 ?? 0.04) and was based primarily upon the telemetry approach. Using high-reward tagging, we estimated different tag reporting rates for state agency and university tagging programs. The combined telemetry - tag return approach can be an effective approach for estimating F and M as long as several key assumptions of the model are met.

  9. To tag or not to tag: animal welfare, conservation and stakeholder considerations in fish tracking studies that use electronic tags

    Energy Technology Data Exchange (ETDEWEB)

    Cooke, Steven J.; Nguyen, Vivian M.; Murchie, Karen J.; Thiem, Jason D.; Donaldson, Michael R.; Hinch, Scott G.; Brown, Richard S.; Fisk, Aaron

    2013-11-01

    The advent and widespread adoption of electronic tags (including biotelemetry and biologging devices) for tracking animals has provided unprecedented information on the biology, management, and conservation of fish in the world’s oceans and inland waters. However, use of these tools is not without controversy. Even when scientific and management objectives may best be achieved using electronic tags, it is increasingly important to further consider other factors such as the welfare of tagged animals (i.e., the role of training and science-based surgical guidelines, anesthetic use, inability to maintain sterile conditions in field environments), the ethics of tagging threatened species vs. using surrogates, stakeholder perspectives on tagging (including aboriginals), as well as use of data emanating from such studies (e.g., by fishers to facilitate exploitation). Failure to do so will have the potential to create conflict and undermine scientific, management and public confidence in the use of this powerful tool. Indeed, there are already a number of examples of where tracking studies using electronic tags have been halted based on concerns raised by researchers, authorities, or stakeholders. Here we present a candid evaluation of several factors that should be considered when determining when to tag or not to tag fish with electronic devices. It is not our objective to judge the merit of previous studies. Rather, we hope to stimulate debate and discussion regarding the use of electronic tags to study fish. Relatedly, there is a need for more research to address these questions (e.g., what level of cleanliness is needed when conducting surgeries, what type of training should be required for fish surgery) including human dimensions studies to understand perspectives of different actors including society as a whole with respect to tagging and tracking studies.

  10. Study to Determine the Biological Feasibility of a New Fish Tagging System, 1998-2000 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Downing, Sandra L.

    2001-05-01

    Since 1984, the National Marine Fisheries Service (NMFS) in cooperation with the Bonneville Power Administration (BPA) has conducted an ongoing research and development project to expand and improve technology for Passive-Integrated-Transponder tags (PIT tags) throughout the Columbia River Basin (CRB). Work conducted as part of this project during 1999-2000 was divided into six individual projects, which are covered separately in this report.

  11. Cormorant predation on PIT-tagged lake fish

    DEFF Research Database (Denmark)

    Skov, Christian; Jepsen, Niels; Baktoft, Henrik

    2014-01-01

    The present study use data from recovered PIT (Passive Integrated Transponder) tags to explore species-and size-specific annual predation rates by cormorants on three common lacustrine fishes (size range 120-367 mm) in a European lake; roach (Rutilus rutilus), common bream (Abramis brama) and perch...... (Perca fluviatilis). In addition, we quantify the level of age/size truncation that cormorant predation could introduce in a population of perch, an important fish for recreational angling as well as for trophic interactions and ecosystem function in European lakes. Based on three years of PIT tagging...... of fish in Lake Viborg and subsequent recoveries of PIT tags from nearby cormorant roosting and breeding sites, we show that cormorants are major predators of roach, bream and perch within the size groups we investigated and for all species larger individuals had higher predation rates. Perch appear...

  12. Pothole tagging system

    CSIR Research Space (South Africa)

    Joubert, D

    2011-11-01

    Full Text Available System (GPS) coordinates of the pothole for use by technical experts and maintenance schedulers. The system will utilise a Microsoft Kinect and a high-speed USB camera as sensing devices....

  13. A Study to Determine the Biological Feasibility of a New Fish Tagging System; Annual Report 1994-1996.

    Energy Technology Data Exchange (ETDEWEB)

    Downing, Sandra L.; Frost, Deborah A.; Jonasson, Bruce F.; Nunnallee, Edmund P.; Peterson, Bradley W.; Prentice, Earl F.; Snell, Glen A.

    1998-11-01

    A multiyear program to evaluate the technical and biological feasibility of a new identification system for salmonids established between the Bonneville Power Administration (BPA) and the National Marine Fisheries Service (NMFS) in 1983.

  14. Gas tagging system development in Japan

    International Nuclear Information System (INIS)

    Sekiguchi, N.; Rindo, H.; Akiyama, T.; Miyazawa, T.; Heki, H.

    1981-05-01

    The Gas tagging method has been considered to be most desirable for a failed fuel location system for the fast breeder reactor, regarding the component reduction in the reactor vessel and rapid location during reactor operation. The gas tagging system has been designed by referring to R and D results obtained in Japan and other countries. The designed system is comprised of tag gas filling pins, cover gas sampling system, tag gas recovery and enrichment system, tag gas analyzer and system control and data handling computers. The main specifications for this system have been decided as follows; 1) Main function is location of failed fuels in core and a part of blanket region, 2) Identification capability is each subassembly, 3) Time for identification is within a few days, 4) Continuous operation with automatic start at fuel failure, 5) Detection sensitivity must cover both gas leak and pin burst. In designing the gas tagging system, the following R and D items were selected; 1) System design study, 2) Tag gas capsule development, 3) Modeling the tag gas behavior in reactor primary cooling system, 4) Tag gas recovery and enrichment system, 5) Computer code development for tag gas isotope ratio change estimation. Details of the Japanese gas tagging system development appear in this paper. (author)

  15. Geolocating fish using Hidden Markov Models and Data Storage Tags

    DEFF Research Database (Denmark)

    Thygesen, Uffe Høgsbro; Pedersen, Martin Wæver; Madsen, Henrik

    2009-01-01

    Geolocation of fish based on data from archival tags typically requires a statistical analysis to reduce the effect of measurement errors. In this paper we present a novel technique for this analysis, one based on Hidden Markov Models (HMM's). We assume that the actual path of the fish is generated...... by a biased random walk. The HMM methodology produces, for each time step, the probability that the fish resides in each grid cell. Because there is no Monte Carlo step in our technique, we are able to estimate parameters within the likelihood framework. The method does not require the distribution...... of inference in state-space models of animals. The technique can be applied to geolocation based on light, on tidal patterns, or measurement of other variables that vary with space. We illustrate the method through application to a simulated data set where geolocation relies on depth data exclusively....

  16. The use of coded wire tags to estimate cormorant predation on fish stocks in an estuary

    DEFF Research Database (Denmark)

    Jepsen, Niels; Klenke, Reinhard; Sonnesen, Per Michael

    2010-01-01

    One of the main obstacles to resolving the conflict between an increasing population of cormorants, Phalacrocorax carbo sinensis, and the fishing industry is the lack of documentation of the effect of the birds’ predation on fish stocks. Tagging and releasing fish with coded wire tags followed...

  17. Scalable Faceted Ranking in Tagging Systems

    Science.gov (United States)

    Orlicki, José I.; Alvarez-Hamelin, J. Ignacio; Fierens, Pablo I.

    Nowadays, web collaborative tagging systems which allow users to upload, comment on and recommend contents, are growing. Such systems can be represented as graphs where nodes correspond to users and tagged-links to recommendations. In this paper we analyze the problem of computing a ranking of users with respect to a facet described as a set of tags. A straightforward solution is to compute a PageRank-like algorithm on a facet-related graph, but it is not feasible for online computation. We propose an alternative: (i) a ranking for each tag is computed offline on the basis of tag-related subgraphs; (ii) a faceted order is generated online by merging rankings corresponding to all the tags in the facet. Based on the graph analysis of YouTube and Flickr, we show that step (i) is scalable. We also present efficient algorithms for step (ii), which are evaluated by comparing their results with two gold standards.

  18. Sensor-based material tagging system

    International Nuclear Information System (INIS)

    Vercellotti, L.C.; Cox, R.W.; Ravas, R.J.; Schlotterer, J.C.

    1991-01-01

    Electronic identification tags are being developed for tracking material and personnel. In applying electronic identification tags to radioactive materials safeguards, it is important to measure attributes of the material to ensure that the tag remains with the material. The addition of a microcontroller with an on-board analog-to-digital converter to an electronic identification tag application-specific integrated-circuit has been demonstrated as means to provide the tag with sensor data. Each tag is assembled into a housing, which serves as a scale for measuring the weight of a paint-can-sized container and its contents. Temperature rise of the can above ambient is also measured, and a piezoelectric detector detects disturbances and immediately puts the tag into its alarm and beacon mode. Radiation measurement was also considered, but the background from nearby containers was found to be excessive. The sensor-based tagging system allows tracking of the material in cans as it is stored in vaults or is moved through the manufacturing process. The paper presents details of the sensor-based material tagging system and describes a demonstration system

  19. PIT-tagging method for small fishes: A case study using sandeel ( Ammodytes tobianus )

    DEFF Research Database (Denmark)

    Jørgensen, Michelle Grace Pinto; Deurs, Mikael van; Butts, Ian

    2017-01-01

    Passive integrated transponder (PIT) tags are commonly used to assess fish movement for use in fisheries management. Here, we investigated physiological and behavioral effects of tagging on sandeels (Ammodytes tobianus) using PIT tags constituting 2.1 ± 0.9% of their body weight. Swimming stamina...

  20. Pop-up Archival Transmitting (PAT) fish tag data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The cooperative tagging center (CTC) began deploying electronic tags in 2002. To date over 300 tags have been deployed. The following species have been monitored:...

  1. Sex identification and PIT-tagging: tools and prospects for studying intersexual differences in freshwater fishes

    DEFF Research Database (Denmark)

    Hulthén, K.; Chapman, B.B.; Nilsson, P.A.

    2014-01-01

    the probability of survival within the size range tagged (119–280 m ), nor were there differences in timing of migration the following season between individuals sexed and tagged in spring and individuals tagged in autumn (i.e. outside the reproductive period). Also, a similar per cent of R. rutilus sexed...... and tagged in spring and tagged in autumn migrated the following season (34·5 and 34·7%). Moreover, long-term recapture data revealed no significant differences in body condition between R. rutilus individuals sexed and tagged in spring, individuals tagged in autumn and unmanipulated individuals....... The observed sex ratio of recaptured fish did not differ from the expected values of equal recapture rates between males and females. Hence, there is no observable evidence for an adverse effect of tagging close to the reproductive period and therefore this method is suitable for studying intersexual...

  2. Effect of anesthetic, tag size, and surgeon experience on postsurgical recovering after implantation of electronic tags in a neotropical fish: Prochilodus lineatus (Valenciennes, 1837 (Characiformes: Prochilodontidae

    Directory of Open Access Journals (Sweden)

    João M. Lopes

    Full Text Available ABSTRACT Implantation of telemetry transmitters in fish can be affected by different parameters. This study aimed to evaluate the effect of type of anesthetic, tag size, and surgeon experience on surgical and postsurgical wound healing in the neotropical fish Prochilodus lineatus . In total, eighty fish were surgically implanted with telemetry transmitters and forty fish were kept as controls. Forty fish were implanted with a small tag and other forty were implanted with a large tag. Similarly, forty fish were anesthetized with eugenol and forty fish were anesthetized by electroanesthesia, and forty surgeries were performed by an expert surgeon and forty surgeries were performed by novice surgeons. At the end of the experimental period seventeen (21.3% tagged fish had postsurgical complications, including death (1.3%, tag expulsion (2.5%, antenna migration (2.5%, and infection (15%. Tag size was the key determinant for postsurgical complications. Surgical details and postsurgical wound healing were not affected by type of anesthetic. Incision size, duration of surgery, and wound area were significantly affected by tag size and surgeon experience, and the number of sutures was significantly affected by tag size only. The results indicate that successful implantation of telemetry transmitters is dependent upon surgeon experience and tag size.

  3. Clone tag detection in distributed RFID systems

    Science.gov (United States)

    Kamaludin, Hazalila; Mahdin, Hairulnizam

    2018-01-01

    Although Radio Frequency Identification (RFID) is poised to displace barcodes, security vulnerabilities pose serious challenges for global adoption of the RFID technology. Specifically, RFID tags are prone to basic cloning and counterfeiting security attacks. A successful cloning of the RFID tags in many commercial applications can lead to many serious problems such as financial losses, brand damage, safety and health of the public. With many industries such as pharmaceutical and businesses deploying RFID technology with a variety of products, it is important to tackle RFID tag cloning problem and improve the resistance of the RFID systems. To this end, we propose an approach for detecting cloned RFID tags in RFID systems with high detection accuracy and minimal overhead thus overcoming practical challenges in existing approaches. The proposed approach is based on consistency of dual hash collisions and modified count-min sketch vector. We evaluated the proposed approach through extensive experiments and compared it with existing baseline approaches in terms of execution time and detection accuracy under varying RFID tag cloning ratio. The results of the experiments show that the proposed approach outperforms the baseline approaches in cloned RFID tag detection accuracy. PMID:29565982

  4. Clone tag detection in distributed RFID systems.

    Science.gov (United States)

    Kamaludin, Hazalila; Mahdin, Hairulnizam; Abawajy, Jemal H

    2018-01-01

    Although Radio Frequency Identification (RFID) is poised to displace barcodes, security vulnerabilities pose serious challenges for global adoption of the RFID technology. Specifically, RFID tags are prone to basic cloning and counterfeiting security attacks. A successful cloning of the RFID tags in many commercial applications can lead to many serious problems such as financial losses, brand damage, safety and health of the public. With many industries such as pharmaceutical and businesses deploying RFID technology with a variety of products, it is important to tackle RFID tag cloning problem and improve the resistance of the RFID systems. To this end, we propose an approach for detecting cloned RFID tags in RFID systems with high detection accuracy and minimal overhead thus overcoming practical challenges in existing approaches. The proposed approach is based on consistency of dual hash collisions and modified count-min sketch vector. We evaluated the proposed approach through extensive experiments and compared it with existing baseline approaches in terms of execution time and detection accuracy under varying RFID tag cloning ratio. The results of the experiments show that the proposed approach outperforms the baseline approaches in cloned RFID tag detection accuracy.

  5. Distribution and Joint Fish-Tag Survival of Juvenile Chinook Salmon Migrating through the Sacramento-San Joaquin River Delta, California, 2008

    Science.gov (United States)

    Holbrook, Christopher M.; Perry, Russell W.; Adams, Noah S.

    2009-01-01

    remained in the San Joaquin River. Once tagged fish entered Old River, only fish collected at two large water conveyance projects and transported through the Delta by truck were detected exiting the Delta, suggesting that this route was the only successful migration pathway for fish that entered Old River. The rate of entrainment of tagged juvenile salmon into Old River was similar to the fraction of San Joaquin River discharge flowing into Old River, which averaged 63 percent but varied tidally and ranged from 33 to 100 percent daily. Although improvements in transmitter battery life are clearly needed, this information will help guide the development of future research and monitoring efforts in this system.

  6. In situ tagging technique for fishes provides insight into growth and movement of invasive lionfish.

    Science.gov (United States)

    Akins, John L; Morris, James A; Green, Stephanie J

    2014-10-01

    Information on fish movement and growth is primarily obtained through the marking and tracking of individuals with external tags, which are usually affixed to anesthetized individuals at the surface. However, the quantity and quality of data obtained by this method is often limited by small sample sizes owing to the time associated with the tagging process, high rates of tagging-related mortality, and displacement of tagged individuals from the initial capture location. To address these issues, we describe a technique for applying external streamer and dart tags in situ, which uses SCUBA divers to capture and tag individual fish on the sea floor without the use of anesthetic. We demonstrate this method for Indo-Pacific lionfish (Pterois volitans/P. miles), species which are particularly vulnerable to barotrauma when transported to and handled at the surface. To test our method, we tagged 161 individuals inhabiting 26 coral reef locations in the Bahamas over a period of 3 years. Our method resulted in no instances of barotrauma, reduced handling and recovery time, and minimal post-tagging release displacement compared with conventional ex situ tag application. Opportunistic resighting and recapture of tagged individuals reveals that lionfish exhibit highly variable site fidelity, movement patterns, and growth rates on invaded coral reef habitats. In total, 24% of lionfish were resighted between 29 and 188 days after tagging. Of these, 90% were located at the site of capture, while the remaining individuals were resighted between 200 m and 1.1 km from initial site of capture over 29 days later. In situ growth rates ranged between 0.1 and 0.6 mm/day. While individuals tagged with streamer tags posted slower growth rates with increasing size, as expected, there was no relationship between growth rate and fish size for individuals marked with dart tags, potentially because of large effects of tag presence on the activities of small bodied lionfish (i.e., lionfish

  7. Peptide-tagged proteins in aqueous two-phase systems

    OpenAIRE

    Nilsson, Anna

    2002-01-01

    This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. However, full effect of the tag as expected from peptide partitioning was not found in the tagged protein. When alkyl-ethylene oxide surfactant was included in a two-polymer system, almost full effect...

  8. A suite of standard post-tagging evaluation metrics can help assess tag retention for field-based fish telemetry research

    Science.gov (United States)

    Gerber, Kayla M.; Mather, Martha E.; Smith, Joseph M.

    2017-01-01

    Telemetry can inform many scientific and research questions if a context exists for integrating individual studies into the larger body of literature. Creating cumulative distributions of post-tagging evaluation metrics would allow individual researchers to relate their telemetry data to other studies. Widespread reporting of standard metrics is a precursor to the calculation of benchmarks for these distributions (e.g., mean, SD, 95% CI). Here we illustrate five types of standard post-tagging evaluation metrics using acoustically tagged Blue Catfish (Ictalurus furcatus) released into a Kansas reservoir. These metrics included: (1) percent of tagged fish detected overall, (2) percent of tagged fish detected daily using abacus plot data, (3) average number of (and percent of available) receiver sites visited, (4) date of last movement between receiver sites (and percent of tagged fish moving during that time period), and (5) number (and percent) of fish that egressed through exit gates. These metrics were calculated for one to three time periods: early (of the study (5 months). Over three-quarters of our tagged fish were detected early (85%) and at the end (85%) of the study. Using abacus plot data, all tagged fish (100%) were detected at least one day and 96% were detected for > 5 days early in the study. On average, tagged Blue Catfish visited 9 (50%) and 13 (72%) of 18 within-reservoir receivers early and at the end of the study, respectively. At the end of the study, 73% of all tagged fish were detected moving between receivers. Creating statistical benchmarks for individual metrics can provide useful reference points. In addition, combining multiple metrics can inform ecology and research design. Consequently, individual researchers and the field of telemetry research can benefit from widespread, detailed, and standard reporting of post-tagging detection metrics.

  9. Tag and Neighbor based Recommender systems for Medical events

    DEFF Research Database (Denmark)

    Bayyapu, Karunakar Reddy; Dolog, Peter

    2010-01-01

    This paper presents an extension of a multifactor recommendation approach based on user tagging with term neighbours. Neighbours of words in tag vectors and documents provide for hitting larger set of documents and not only those matching with direct tag vectors or content of the documents. Tag...... in the situations where the quality of tags is lower. We discuss the approach on the examples from the existing Medworm system to indicate the usefulness of the approach....

  10. Tagged at first listen: an examination of social tagging practices in a music recommender system

    Directory of Open Access Journals (Sweden)

    Audrey Laplante

    2015-01-01

    Full Text Available http://dx.doi.org/10.5007/1518-2924.2015v20nesp1p33 Social tagging has become a very common way to index different types of resources on the web. Less prevalent in music than in other domains, social tagging is nevertheless used in a popular recommender system, Last.fm. Although the number of publications on tagging and folksonomies has exploded in the last few years, music tagging is still not well studied. In this paper, we present a study of tagging practices of Last.fm users. We examine the social tagging of songs during the first three months after their release. Our analysis shows that the release of a song triggers a burst in tagging activity that lasts two weeks, after what it decreases sharply and then remains fairly constant for the next ten weeks. We also find that a majority of songs do not get tagged during the first week and that tagging was positively related to popularity. Finally, we find that tags that have been frequently applied to a given song are more likely to be genre related, shorter in length, and relatively objective than tags that have been applied only once.

  11. Estimating movement and survival rates of a small saltwater fish using autonomous antenna receiver arrays and passive integrated transponder tags

    Science.gov (United States)

    Rudershausen, Paul J.; Buckel, Jeffery A.; Dubreuil, Todd; O'Donnell, Matthew J.; Hightower, Joseph E.; Poland, Steven J.; Letcher, Benjamin H.

    2014-01-01

    We evaluated the performance of small (12.5 mm long) passive integrated transponder (PIT) tags and custom detection antennas for obtaining fine-scale movement and demographic data of mummichog Fundulus heteroclitus in a salt marsh creek. Apparent survival and detection probability were estimated using a Cormack Jolly Seber (CJS) model fitted to detection data collected by an array of 3 vertical antennas from November 2010 to March 2011 and by a single horizontal antenna from April to August 2011. Movement of mummichogs was monitored during the period when the array of vertical antennas was used. Antenna performance was examined in situ using tags placed in wooden dowels (drones) and in live mummichogs. Of the 44 tagged fish, 42 were resighted over the 9 mo monitoring period. The in situ detection probabilities of the drone and live mummichogs were high (~80-100%) when the ambient water depth was less than ~0.8 m. Upstream and downstream movement of mummichogs was related to hourly water depth and direction of tidal current in a way that maximized time periods over which mummichogs utilized the intertidal vegetated marsh. Apparent survival was lower during periods of colder water temperatures in December 2010 and early January 2011 (median estimate of daily apparent survival = 0.979) than during other periods of the study (median estimate of daily apparent survival = 0.992). During late fall and winter, temperature had a positive effect on the CJS detection probability of a tagged mummichog, likely due to greater fish activity over warmer periods. During the spring and summer, this pattern reversed possibly due to mummichogs having reduced activity during the hottest periods. This study demonstrates the utility of PIT tags and continuously operating autonomous detection systems for tracking fish at fine temporal scales, and improving estimates of demographic parameters in salt marsh creeks that are difficult or impractical to sample with active fishing gear.

  12. Guidelines for calculating and enhancing detection efficiency of PIT tag interrogation systems

    Science.gov (United States)

    Connolly, Patrick J.

    2010-01-01

    With increasing use of passive integrated transponder (PIT) tags and reliance on stationary PIT tag interrogation systems to monitor fish populations, guidelines are offered to inform users how best to use limited funding and human resources to create functional systems that maximize a desired level of detection and precision. The estimators of detection efficiency and their variability as described by Connolly et al. (2008) are explored over a span of likely performance metrics. These estimators were developed to estimate detection efficiency without relying on a known number of fish passing the system. I present graphical displays of the results derived from these estimators to show the potential efficiency and precision to be gained by adding an array or by increasing the number of PIT-tagged fish expected to move past an interrogation system.

  13. Guidelines to indirectly measure and enhance detection efficiency of stationary PIT tag interrogation systems in streams

    Science.gov (United States)

    Connolly, Patrick J.; Wolf, Keith; O'Neal, Jennifer S.

    2010-01-01

    With increasing use of passive integrated transponder (PIT) tags and reliance on stationary PIT tag interrogation systems to monitor fish populations, guidelines are offered to inform users how best to use limited funding and human resources to create functional systems that maximize a desired level of detection and precision. The estimators of detection efficiency and their variability as described by Connolly et al. (2008) are explored over a span of likely performance metrics. These estimators were developed to estimate detection efficiency without relying on a known number of fish passing the system. I present graphical displays of the results derived from these estimators to show the potential efficiency and precision to be gained by adding an array or by increasing the number of PIT-tagged fish expected to move past an interrogation system.

  14. Antibiotic use during the intracoelomic implantation of electronic tags into fish

    Science.gov (United States)

    Mulcahy, D.M.

    2011-01-01

    The use of antibiotics, in particular, the use of a single dose of antibiotics during electronic tag implantation is of unproven value, and carries with it the potential for the development of antibiotic resistance in bacteria and the alteration of the immune response of the fish. Antibiotic use during electronic tag implantation must conform to relevant drug laws and regulations in the country where work is being done, including the requirements for withdrawal times before human consumption is a possibility. Currently, the choice of antibiotics (most often tetracycline or oxytetracycline) and the use of a single dose of the drug are decisions made without knowledge of the basic need for antibiotic usage and of the bacteria involved in infections that occur following electronic tag implantation. Correct perioperative use of an antibiotic is to apply the drug to the animal before surgery begins, to assure serum and tissue levels of the drug are adequate before the incision is made. However, the most common perioperative application of antibiotics during implantation of an electronic tag is to delay the administration of the drug, injecting it into the coelom after the electronic tag is inserted, just prior to closure of the incision. There is little empirical evidence that the present application of antibiotics in fish being implanted with electronic tags is of value. Improvements should first be made to surgical techniques, especially the use of aseptic techniques and sterilized instruments and electronic tags, before resorting to antibiotics. ?? 2010 Springer Science+Business Media B.V.(outside the USA).

  15. Improving Recommendations in Tag-based Systems with Spectral Clustering of Tag Neighbors

    DEFF Research Database (Denmark)

    Pan, Rong; Xu, Guandong; Dolog, Peter

    2012-01-01

    Tag as a useful metadata reflects the collaborative and conceptual features of documents in social collaborative annotation systems. In this paper, we propose a collaborative approach for expanding tag neighbors and investigate the spectral clustering algorithm to filter out noisy tag neighbors...... in order to get appropriate recommendation for users. The preliminary experiments have been conducted on MovieLens dataset to compare our proposed approach with the traditional collaborative filtering recommendation approach and naive tag neighbors expansion approach in terms of precision, and the result...... demonstrates that our approach could considerably improve the performance of recommendations....

  16. Group Discovery in a CollaborativeTagging System

    OpenAIRE

    Chen, Zijian

    2007-01-01

    Tagging refers to the process of adding metadata to describe things by usingone or several words. Collaborative Tagging systems, which allow different webusers to tag web content like weblogs, pictures, and bookmarks and so on, haverecently gained great popularity on internet. There are already a greatvariety of debates on internet of the advantages and disadvantages ofcollaborative tagging systems from the aspect of information organizing. Inthis paper, we primarily focus on a collaborative ...

  17. Surgical implantation techniques for electronic tags in fish

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, Glenn N.; Cooke, Steven J.; Brown, Richard S.; Deters, Katherine A.

    2011-01-01

    Intracoelomic implantation of transmitters into fish requires making a surgical incision, incision closure, and other surgery related techniques; however, the tools and techniques used in the surgical process vary widely. We review the available literature and focus on tools and techniques used for conducting surgery on juvenile salmonids because of the large amount of research that is conducted on them. The use of sterilized surgical instruments properly selected for a given size of fish will minimize tissue damage and infection rates, and speed the wound healing of fish implanted with transmitters. For the implantation of transmitters into small fish, the optimal surgical methods include making an incision on the ventral midline along the linea alba (for studies under 1 month), protecting the viscera (by lifting the skin with forceps while creating the incision), and using absorbable monofilament suture with a small-swaged-on swaged-on tapered or reverse-cutting needle. Standardizing the implantation techniques to be used in a study involving particular species and age classes of fish will improve survival and transmitter retention while allowing for comparisons to be made among studies and across multiple years. This review should be useful for researchers working on juvenile salmonids and other sizes and species of fish.

  18. A Personalized Tag-Based Recommendation in Social Web Systems

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2009-01-01

    -based recommender system which suggests similar Web pages based on the similarity of their tags from a Web 2.0 tagging application. The proposed approach extends the basic similarity calculus with external factors such as tag popularity, tag representativeness and the affinity between user and tag. In order...... to study and evaluate the recommender system, we have conducted an experiment involving 38 people from 12 countries using data from Del.icio.us , a social bookmarking web system on which users can share their personal bookmarks......Tagging activity has been recently identified as a potential source of knowledge about personal interests, preferences, goals, and other attributes known from user models. Tags themselves can be therefore used for finding personalized recommendations of items. In this paper, we present a tag...

  19. Extracting Usage Patterns and the Analysis of Tag Connection Dynamics within Collaborative Tagging Systems

    Directory of Open Access Journals (Sweden)

    Daniel MICAN

    2013-01-01

    Full Text Available Collaborative tagging has become a very popular way of annotation, thanks to the fact that any entity may be labeled by any individual based on his own reason. In this paper we present the results of the case study carried out on the basis of data gathered at different time intervals from the social tagging system developed and implemented on Întelepciune.ro. Analyzing collective data referring to the way in which community members associate different tags, we have observed that between tags, links are formed which become increasingly stable with the passing of time. Following the application of methodology specific to network analysis, we have managed to extract information referring to tag popularity, their influence within the network and the degree to which a tag depends upon another. As such, we have succeeded in determining different semantic structures within the collective tagging system and see their evolution at different stages in time. Furthermore, we have pictured the way in which tag rec-ommendations can be executed and that they can be integrated within recommendation sys-tems. Thus, we will be able to identify experts and trustworthy content based on different cat-egories of interest.

  20. A suite of standard post-tagging evaluation metrics can help assess tag retention for field-based fish telemetry research

    Science.gov (United States)

    Gerber, Kayla M.; Mather, Martha E.; Smith, Joseph M.

    2017-01-01

    Telemetry can inform many scientific and research questions if a context exists for integrating individual studies into the larger body of literature. Creating cumulative distributions of post-tagging evaluation metrics would allow individual researchers to relate their telemetry data to other studies. Widespread reporting of standard metrics is a precursor to the calculation of benchmarks for these distributions (e.g., mean, SD, 95% CI). Here we illustrate five types of standard post-tagging evaluation metrics using acoustically tagged Blue Catfish (Ictalurus furcatus) released into a Kansas reservoir. These metrics included: (1) percent of tagged fish detected overall, (2) percent of tagged fish detected daily using abacus plot data, (3) average number of (and percent of available) receiver sites visited, (4) date of last movement between receiver sites (and percent of tagged fish moving during that time period), and (5) number (and percent) of fish that egressed through exit gates. These metrics were calculated for one to three time periods: early ( 5 days early in the study. On average, tagged Blue Catfish visited 9 (50%) and 13 (72%) of 18 within-reservoir receivers early and at the end of the study, respectively. At the end of the study, 73% of all tagged fish were detected moving between receivers. Creating statistical benchmarks for individual metrics can provide useful reference points. In addition, combining multiple metrics can inform ecology and research design. Consequently, individual researchers and the field of telemetry research can benefit from widespread, detailed, and standard reporting of post-tagging detection metrics.

  1. Parasites as biological tags of fish stocks: a meta-analysis of their discriminatory power.

    Science.gov (United States)

    Poulin, Robert; Kamiya, Tsukushi

    2015-01-01

    The use of parasites as biological tags to discriminate among marine fish stocks has become a widely accepted method in fisheries management. Here, we first link this approach to its unstated ecological foundation, the decay in the similarity of the species composition of assemblages as a function of increasing distance between them, a phenomenon almost universal in nature. We explain how distance decay of similarity can influence the use of parasites as biological tags. Then, we perform a meta-analysis of 61 uses of parasites as tags of marine fish populations in multivariate discriminant analyses, obtained from 29 articles. Our main finding is that across all studies, the observed overall probability of correct classification of fish based on parasite data was about 71%. This corresponds to a two-fold improvement over the rate of correct classification expected by chance alone, and the average effect size (Zr = 0·463) computed from the original values was also indicative of a medium-to-large effect. However, none of the moderator variables included in the meta-analysis had a significant effect on the proportion of correct classification; these moderators included the total number of fish sampled, the number of parasite species used in the discriminant analysis, the number of localities from which fish were sampled, the minimum and maximum distance between any pair of sampling localities, etc. Therefore, there are no clear-cut situations in which the use of parasites as tags is more useful than others. Finally, we provide recommendations for the future usage of parasites as tags for stock discrimination, to ensure that future applications of the method achieve statistical rigour and a high discriminatory power.

  2. Extending a Hybrid Tag-Based Recommender System with Personalization

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2010-01-01

    extension for a hybrid tag-based recommender system, which suggests similar Web pages based on the similarity of their tags. The semantic extension aims at discovering tag relations which are not considered in basic syntax similarity. With the goal of generating more semantically grounded recommendations......, the proposal extends a hybrid tag-based recommender system with a semantic factor, which looks for tag relations in different semantic sources. In order to evaluate the benefits acquired with the semantic extension, we have compared the new findings with results from a previous experiment involving 38 people......Tagging activity has been recently identified as a potential source of knowledge about personal interests, preferences, goals, and other attributes known from user models. Tags themselves can be therefore used for finding personalized recommendations of items. This paper proposes a semantic...

  3. An introduction to the practical and ethical perspectives on the need to advance and standardize the intracoelomic surgical implantation of electronic tags in fish

    Science.gov (United States)

    Brown, R.S.; Eppard, M.B.; Murchie, K.J.; Nielsen, J.L.; Cooke, S.J.

    2011-01-01

    The intracoelomic surgical implantation of electronic tags (including radio and acoustic telemetry transmitters, passive integrated transponders and archival biologgers) is frequently used for conducting studies on fish. Electronic tagging studies provide information on the spatial ecology, behavior and survival of fish in marine and freshwater systems. However, any surgical procedure, particularly one where a laparotomy is performed and the coelomic cavity is opened, has the potential to alter the survival, behavior or condition of the animal which can impair welfare and introduce bias. Given that management, regulatory and conservation decisions are based on the assumption that fish implanted with electronic tags have similar fates and behavior relative to untagged conspecifics, it is critical to ensure that best surgical practices are being used. Also, the current lack of standardized surgical procedures and reporting of specific methodological details precludes cross-study and cross-year analyses which would further progress the field of fisheries science. This compilation of papers seeks to identify the best practices for the entire intracoelomic tagging procedure including pre- and post-operative care, anesthesia, wound closure, and use of antibiotics. Although there is a particular focus on salmonid smolts given the large body of literature available on that group, other life-stages and species of fish are discussed where there is sufficient knowledge. Additional papers explore the role of the veterinarian in fish surgeries, the need for minimal standards in the training of fish surgeons, providing a call for more complete and transparent procedures, and identifying trends in procedures and research needs. Collectively, this body of knowledge should help to improve data quality (including comparability and repeatability), enhance management and conservation strategies, and maintain the welfare status of tagged fish. ?? 2010 Springer Science+Business Media B.V.

  4. External tagging does not affect the feeding behavior of a coral reef fish, Chaetodon vagabundus (Pisces: Chaetodontidae)

    KAUST Repository

    Berumen, Michael L.

    2009-11-10

    Increasingly, the ability to recognize individual fishes is important for studies of population dynamics, ecology, and behavior. Although a variety of methods exist, external tags remain one of the most widely applied because they are both effective and cost efficient. However, a key assumption is that neither the tagging procedure nor the presence of a tag negatively affects the individual. While this has been demonstrated for relatively coarse metrics such as growth and survival, few studies have examined the impact of tags and tagging on more subtle aspects of behavior. We tagged adult vagabond butterflyfish (Chaetodon vagabundus) occupying a 30-ha insular reef in Kimbe Bay, Papua New Guinea, using a commonly-utilized t-bar anchor tag. We quantified and compared feeding behavior (bite rate), which is sensitive to stress, of tagged and untagged individuals over four separate sampling periods spanning 4 months post-tagging. Bite rates did not differ between tagged and untagged individuals at each sampling period and, combined with additional anecdotal observations of normal pairing behavior and successful reproduction, suggest that tagging did not adversely affect individuals. © Springer Science+Business Media B.V. 2009.

  5. InkTag: Secure Applications on an Untrusted Operating System.

    Science.gov (United States)

    Hofmann, Owen S; Kim, Sangman; Dunn, Alan M; Lee, Michael Z; Witchel, Emmett

    2013-01-01

    InkTag is a virtualization-based architecture that gives strong safety guarantees to high-assurance processes even in the presence of a malicious operating system. InkTag advances the state of the art in untrusted operating systems in both the design of its hypervisor and in the ability to run useful applications without trusting the operating system. We introduce paraverification , a technique that simplifies the InkTag hypervisor by forcing the untrusted operating system to participate in its own verification. Attribute-based access control allows trusted applications to create decentralized access control policies. InkTag is also the first system of its kind to ensure consistency between secure data and metadata, ensuring recoverability in the face of system crashes.

  6. InkTag: Secure Applications on an Untrusted Operating System

    Science.gov (United States)

    Hofmann, Owen S.; Kim, Sangman; Dunn, Alan M.; Lee, Michael Z.; Witchel, Emmett

    2014-01-01

    InkTag is a virtualization-based architecture that gives strong safety guarantees to high-assurance processes even in the presence of a malicious operating system. InkTag advances the state of the art in untrusted operating systems in both the design of its hypervisor and in the ability to run useful applications without trusting the operating system. We introduce paraverification, a technique that simplifies the InkTag hypervisor by forcing the untrusted operating system to participate in its own verification. Attribute-based access control allows trusted applications to create decentralized access control policies. InkTag is also the first system of its kind to ensure consistency between secure data and metadata, ensuring recoverability in the face of system crashes. PMID:24429939

  7. Advancing the surgical implantation of electronic tags in fish: a gap analysis and research agenda based on a review of trends in intracoelomic tagging effects studies

    Energy Technology Data Exchange (ETDEWEB)

    Cooke, Steven J.; Woodley, Christa M.; Eppard, M. B.; Brown, Richard S.; Nielsen, Jennifer L.

    2011-03-08

    Early approaches to surgical implantation of electronic tags in fish were often through trial and error, however, in recent years there has been an interest in using scientific research to identify techniques and procedures that improve the outcome of surgical procedures and determine the effects of tagging on individuals. Here we summarize the trends in 108 peer-reviewed electronic tagging effect studies focused on intracoleomic implantation to determine opportunities for future research. To date, almost all of the studies have been conducted in freshwater, typically in laboratory environments, and have focused on biotelemetry devices. The majority of studies have focused on salmonids, cyprinids, ictalurids and centrarchids, with a regional bias towards North America, Europe and Australia. Most studies have focused on determining whether there is a negative effect of tagging relative to control fish, with proportionally fewer that have contrasted different aspects of the surgical procedure (e.g., methods of sterilization, incision location, wound closure material) that could advance the discipline. Many of these studies included routine endpoints such as mortality, growth, healing and tag retention, with fewer addressing sublethal measures such as swimming ability, predator avoidance, physiological costs, or fitness. Continued research is needed to further elevate the practice of electronic tag implantation in fish in order to ensure that the data generated are relevant to untagged conspecifics (i.e., no long-term behavioural or physiological consequences) and the surgical procedure does not impair the health and welfare status of the tagged fish. To that end, we advocate for i) rigorous controlled manipulations based on statistical designs that have adequate power, account for inter-individual variation, and include controls and shams, ii) studies that transcend the laboratory and the field with more studies in marine waters, iii) incorporation of knowledge and

  8. Comparison of the movement and recapture of salmonid fishes tagged at two power plants

    International Nuclear Information System (INIS)

    Romberg, G.P.; Thommes, M.M.

    1974-01-01

    Fish tagging studies were conducted in the vicinity of Point Beach Nuclear Plant and Waukegan Power Plant to determine whether there were any seasonal or site specific differences in the residence behavior of salmonids at thermal discharges. Results showed that there were differences in the abundance and time of peak abundance of trout and salmon at the two power plant discharges. Certain species reacted differently to the two discharges probably as a result of maturity and water temperature. Salmonids did not appear to remain at either discharge for long periods. Direction of migration was affected by stocking location and water temperature

  9. Use of PIT tag and underwater video recording in assessing estuarine fish movement in a high intertidal mangrove and salt marsh creek

    Science.gov (United States)

    Meynecke, Jan-Olaf; Poole, Geoffrey C.; Werry, Jonathan; Lee, Shing Yip

    2008-08-01

    We assessed movement patterns in relation to habitat availability (reflected by the extent of tidal flooding) for several commercially and recreationally important species in and out of a small mangrove creek within the subtropical Burrum River estuary (25°10'S 152°37'E) in Queensland, Australia. Movement patterns of Acanthopagrus australis, Pomadasys kaakan, Lutjanus russelli and Mugil cephalus were examined between December 2006 and April 2007 using a stationary passive integrated transponder (PIT) system adapted for saline environments (30-38 ppt) and underwater digital video cameras (DVCs). This is the second known application of a stationary PIT tag system to studying fish movement in estuarine environments. The transponder system was set in place for 104 days and recorded >5000 detections. Overall 'recapture' rate of tagged fish by the transponder system was >40%. We used PIT tags implanted in a total of 75 fish from a tidal creek connected to the main channel of the estuary. We also developed a high-resolution digital elevation (2.5 m cell size) model of the estuary derived from airborne light detection and ranging (LIDAR) and aerial imagery to estimate inundation dynamics within the tidal creek, and related the timing of inundation in various habitats to the timing of fish immigration to and emigration from the creek. Over 50% of all tagged fish were moving in and out of the creek at a threshold level when 50% of the mangrove forest became flooded. Individuals of all four species moved into and out of the tidal creek repeatedly at different times depending on species and size, indicating strong residential behaviour within the estuary. The main activity of fishes was at night time. Manual interpretation of video from >700 fish sightings at three different mangrove sites confirmed the findings of the stationary PIT system, that the function of shelter vs food in mangrove habitat may be size dependent. Our established techniques assess the spatial ecology

  10. Social and Behavioral Aspects of a Tag-Based Recommender System

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2009-01-01

    Collaborative tagging has emerged as a useful means to organize and share resources on the Web. Recommender systems have been utilized tags for identifying similar resources and generate personalized recommendations. In this paper, we analyze social and behavioral aspects of a tag-based recommender...... system which suggests similar Web pages based on the similarity of their tags. Tagging behavior and language anomalies in tagging activities are some aspects examined from an experiment involving 38 people from 12 countries....

  11. RFID Torque Sensing Tag System for Fasteners

    Science.gov (United States)

    Fink, Patrick W. (Inventor); Lin, Gregory Y. (Inventor); Ngo, Phong H. (Inventor); Kennedy, Timothy F. (Inventor)

    2016-01-01

    The present invention provides an RFID-based torque sensor that can be used to quickly monitor off the shelf fasteners including fasteners that are used in expensive satellites or other uses where fastener failure can be very costly. In one embodiment, an antenna, RFID ring and spring comprise a sensor tag that can be interrogated with an interrogation signal produced by an interrogator device. When sufficient torque is applied to the fastener, an RFID circuit is connected, and produces a radio frequency (RF) signal that can be read by the interrogator. In one embodiment, the RFID circuit does not transmit when the spring member is not compressed, thereby indicating insufficient tensioning of the fastener. The present invention offers the ability to remotely, quickly, and inexpensively verify that any number of fasteners are torqued properly upon initial installation. Where applicable, the present invention allows low cost monitoring over the life of the fastener.

  12. Effects of Pile Driving on the Residency and Movement of Tagged Reef Fish.

    Directory of Open Access Journals (Sweden)

    Joseph D Iafrate

    Full Text Available The potential effects of pile driving on fish populations and commercial fisheries have received significant attention given the prevalence of pile driving occurring in coastal habitats throughout the world. Behavioral impacts of sound generated from these activities on fish typically have a greater area of influence than physical injury, and may therefore adversely affect a greater portion of the local population. This study used acoustic telemetry to assess the movement, residency, and survival of 15 sheepshead (Archosargus probatocephalus and 10 grey snapper (Lutjanus griseus in Port Canaveral, Florida, USA, in response to 35 days of pile driving at a wharf complex. No obvious signs of mortality or injury to tagged fish were evident from the data. Received sound pressure levels from pile strikes on the interior of the wharf, where reef fish primarily occur, were on average 152-157 dB re 1 μPa (peak. No significant decrease in sheepshead daytime residency was observed during pile driving within the central portion of the wharf and area of highest sound exposure, and no major indicators of displacement from the exposure wharf with the onset of pile driving were observed. There was evidence of potential displacement from the exposure wharf that coincided with the start of pile driving observed for 2 out of 4 grey snapper, along with a decrease in daytime residency for a subset of this species with high site fidelity prior to the event. Results indicate that snapper may be more likely to depart an area of pile driving disturbance more readily than sheepshead, but were less at risk for behavioral impact given the lower site fidelity of this species.

  13. Comparative injury, adipose fin mark quality, and tag retention of spring Chinook Salmon marked and coded wire tagged by an automated trailer and manual trailer at Carson National Fish Hatchery: October 2008

    Data.gov (United States)

    Department of the Interior — The United States Fish and Wildlife Service’s Columbia River Fisheries Program Office has been marking and tagging salmon with automated trailers consistently since...

  14. Training considerations for the intracoelomic implantation of electronic tags in fish with a summary of common surgical errors

    Energy Technology Data Exchange (ETDEWEB)

    Cooke, Steven J.; Wagner, Glenn N.; Brown, Richard S.; Deters, Katherine A.

    2011-01-01

    Training is a fundamental part of all scientific and technical disciplines. This is particularly true for all types of surgeons. For surgical procedures, a number of skills are necessary to reduce mistakes. Trainees must learn an extensive yet standardized set of problem-solving and technical skills to handle challenges as they arise. There are currently no guidelines or consistent training methods for those intending to implant electronic tags in fish; this is surprising, considering documented cases of negative consequences of fish surgeries and information from studies having empirically tested fish surgical techniques. Learning how to do fish surgery once is insufficient for ensuring the maintenance or improvement of surgical skill. Assessment of surgical skills is rarely incorporated into training, and is needed. Evaluation provides useful feedback that guides future learning, fosters habits of self-reflection and self-remediation, and promotes access to advanced training. Veterinary professionals should be involved in aspects of training to monitor basic surgical principles. We identified attributes related to knowledge, understanding, and skill that surgeons must demonstrate prior to performing fish surgery including a “hands-on” assessment using live fish. Included is a summary of common problems encountered by fish surgeons. We conclude by presenting core competencies that should be required as well as outlining a 3-day curriculum for training surgeons to conduct intracoelomic implantation of electronic tags. This curriculum could be offered through professional fisheries societies as professional development courses.

  15. 2706-T Complex Distributed Control System Tag and Setpoint List

    International Nuclear Information System (INIS)

    PRATT, D.A.

    1999-01-01

    The 2706-T Distributed Control System (DCS) interfaces with field equipment through analog and digital input and output signals that are terminated at a programmable logic controller (PLC). The Tag names and addresses of the input and output signals are listed in this document as well as setpoint values assigned to fixed inputs

  16. EnTagRec : an enhanced tag recommendation system for software information sites

    NARCIS (Netherlands)

    Wang, S.; Lo, D.; Vasilescu, B.N.; Serebrenik, A.

    2014-01-01

    Software engineers share experiences with modern technologies by means of software information sites, such as STACK OVERFLOW. These sites allow developers to label posted content, referred to as software objects, with short descriptions, known as tags. However, tags assigned to objects tend to be

  17. Comparative Performance of Acoustic-tagged and PIT-tagged Juvenile Salmonids

    Energy Technology Data Exchange (ETDEWEB)

    Hockersmith, Eric E.; Brown, Richard S.; Liedtke, Theresa L.

    2008-02-01

    Numerous research tools and technologies are currently being used to evaluate fish passage and survival to determine the impacts of the Federal Columbia River Power System (FCRPS) on endangered and threatened juvenile salmonids, including PIT tags, balloon tags, hydroacoustic evaluations, radio telemetry, and acoustic telemetry. Each has advantages and disadvantages, but options are restricted in some situations because of limited capabilities of a specific technology, lack of detection capability downstream, or availability of adequate numbers of fish. However, there remains concern about the comparative effects of the tag or the tagging procedure on fish performance. The recently developed Juvenile Salmonid Acoustic Telemetry System (JSATS) acoustic transmitter is the smallest active acoustic tag currently available. The goal of this study was to determine whether fish tagged with the JSATS acoustic-telemetry tag can provide unbiased estimates of passage behavior and survival within the performance life of the tag. We conducted both field and laboratory studies to assess tag effects. For the field evaluation we released a total of 996 acoustic-tagged fish in conjunction with 21,026 PIT-tagged fish into the tailrace of Lower Granite Dam on 6 and 13 May. Travel times between release and downstream dams were not significantly different for the majority of the reaches between acoustic-tagged and PIT-tagged fish. In addition to the field evaluation, a series of laboratory experiments were conducted to determine if growth and survival of juvenile Chinook salmon surgically implanted with acoustic transmitters is different than untagged or PIT tagged juvenile Chinook salmon. Only yearling fish with integrated and non-integrated transmitters experienced mortalities, and these were low (<4.5%). Mortality among sub-yearling control and PIT-tag treatments ranged up to 7.7% while integrated and non-integrated treatments had slightly higher rates (up to 8.3% and 7

  18. TRSDL: Tag-Aware Recommender System Based on Deep Learning–Intelligent Computing Systems

    Directory of Open Access Journals (Sweden)

    Nan Liang

    2018-05-01

    Full Text Available In recommender systems (RS, many models are designed to predict ratings of items for the target user. To improve the performance for rating prediction, some studies have introduced tags into recommender systems. Tags benefit RS considerably, however, they are also redundant and ambiguous. In this paper, we propose a hybrid deep learning model TRSDL (tag-aware recommender system based on deep learning to improve the performance of tag-aware recommender systems (TRS. First, TRSDL uses pre-trained word embeddings to represent user-defined tags, and constructs item and user profiles based on the items’ tags set and users’ tagging behaviors. Then, it utilizes deep neural networks (DNNs and recurrent neural networks (RNNs to extract the latent features of items and users, respectively. Finally, it predicts ratings from these latent features. The model not only addresses tag limitations and takes advantage of semantic tag information but also learns more advanced implicit features via deep structures. We evaluated our proposed approach and several baselines on MovieLens-20 m, and the experimental results demonstrate that TRSDL significantly outperforms all the baselines (including the state-of-the-art models BiasedMF and I-AutoRec. In addition, we also explore the impacts of network depth and type on model performance.

  19. Development of a New Delivery and Attachment System for Tagging Large Cetaceans

    National Research Council Canada - National Science Library

    Harvey, James

    1997-01-01

    .... We developed a new method of delivering tags for placement on large whales. Sea lions were trained to carry a harness and attached camera system along with carrying a suction-cup tag in a mouth piece...

  20. An Extended-Tag-Induced Matrix Factorization Technique for Recommender Systems

    Directory of Open Access Journals (Sweden)

    Huirui Han

    2018-06-01

    Full Text Available Social tag information has been used by recommender systems to handle the problem of data sparsity. Recently, the relationships between users/items and tags are considered by most tag-induced recommendation methods. However, sparse tag information is challenging to most existing methods. In this paper, we propose an Extended-Tag-Induced Matrix Factorization technique for recommender systems, which exploits correlations among tags derived by co-occurrence of tags to improve the performance of recommender systems, even in the case of sparse tag information. The proposed method integrates coupled similarity between tags, which is calculated by the co-occurrences of tags in the same items, to extend each item’s tags. Finally, item similarity based on extended tags is utilized as an item relationship regularization term to constrain the process of matrix factorization. MovieLens dataset and Book-Crossing dataset are adopted to evaluate the performance of the proposed algorithm. The results of experiments show that the proposed method can alleviate the impact of tag sparsity and improve the performance of recommender systems.

  1. Bus and Tag Terminators for IBM system/360

    CERN Multimedia

    Control units were connected to the channels with "Bus and Tag" cable pairs. The bus cables carried the address and data information and the tag cables identified what data was on the bus. There were three general types of bus-and-tag cables produced by IBM.

  2. Parasites as biological tags of marine, freshwater and anadromous fishes in North America from the Tropics to the Arctic.

    Science.gov (United States)

    Marcogliese, David J; Jacobson, Kym C

    2015-01-01

    Parasites have been considered as natural biological tags of marine fish populations in North America for almost 75 years. In the Northwest Atlantic, the most studied species include Atlantic cod (Gadus morhua), Atlantic herring (Clupea harengus) and the redfishes (Sebastes spp.). In the North Pacific, research has centred primarily on salmonids (Oncorhynchus spp.). However, parasites have been applied as tags for numerous other pelagic and demersal species on both the Atlantic and Pacific coasts. Relatively few studies have been undertaken in the Arctic, and these were designed to discriminate anadromous and resident salmonids (Salvelinus spp.). Although rarely applied in fresh waters, parasites have been used to delineate certain fish stocks within the Great Lakes-St Lawrence River basin. Anisakid nematodes and the copepod Sphyrion lumpi frequently prove useful indicators in the Northwest Atlantic, while myxozoan parasites prove very effective on the coast and open seas of the Pacific Ocean. Relative differences in the ability of parasites to discriminate between fish stocks on the Pacific and Atlantic coasts may be due to oceanographic and bathymetric differences between regions. Molecular techniques used to differentiate populations and species of parasites show promise in future applications in the field.

  3. A proposal for an international tagging system for radioactive materials

    International Nuclear Information System (INIS)

    Bahran, M.

    2001-01-01

    Full text: In an earlier paper based on our experience with lost or abundant radiation sources, we have discussed the need of an international tagging system for radioactive materials for the purpose of insuring trackability, accountability and safety. In this paper we take our discussion further by presenting a proposal of establishing such an international tagging system for radioactive materials which we call ITS-RM. The elements of ITS-RM: IAEA is to be the international authority in charge of ITS-RM; Each 'significant' radiation source is to be labeled with a unique number at the point of production. This number is identified as ITS-RM number; The term 'significant' can be defined in light of current international standards; As each source is naturally possessed by some legal notion or international entity, each time this entity is changed (e.g. the source is sold, resold, transported, decommissioned etc.) the process of changing hand is registered at IAEA via the ITS-RM number; No source is to change hands without triggering ITS-RM; Even if a source finds its way to a waste facility it will remain tagged indefinitely. The requirement for ITS-RM: Legal requirement: this issue needs to be studied to find out weather anew international treaty needs to be established or ITS-RM can be applied under existing treaties; Organizational requirements: This can be easily done with the framework if IAEA and national counterparts; Consultation requirements: ITS-RM can only work if it is done through complete consultation and cooperation with manufactures. Old and current sources: An effort is to be made to survey and tag current sources; Priorities will be given as follows: first to new sources second to current sources the comes last old sources, particularly those in storage facilities; ITS-RM is not to compete with any existing safety system, national or internationally, to the contrary is to support safety. ITS-RM advantages are many but in particular we have

  4. Cooperative Tagging Center (CTC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Cooperative Tagging Center (CTC) began as the Cooperative Game Fish Tagging Program (GTP) at Woods Hole Oceanographic Institute (WHOI) in 1954. The GTP was...

  5. Development of the EURITRACK tagged neutron inspection system

    International Nuclear Information System (INIS)

    Perot, B.; Carasco, C.; Bernard, S.

    2007-01-01

    The EURopean Illicit TRAfficing Countermeasures Kit (EURITRACK) project is part of the 6th European Union Framework Program. It aims at developing a Tagged Neutron Inspection System (TNIS) to detect illicit materials, such as explosives and narcotics, in cargo containers. Fast neutron induced reactions produce specific gamma-rays used to determine the chemical composition of the inspected material. The associated particle technique is employed to precisely locate the interaction points of the neutrons. A new deuterium-tritium neutron generator has been developed, including a pixelized alpha particle detector. The TNIS also comprises high-efficiency fast neutron and gamma-ray detectors, a dedicated front-end electronics and an integrated software to entirely drive the system and automatically process the data. Most components have been integrated during last months at Institute Ruder Boskovic, in Zagreb, Croatia. An overview of the TNIS and of its preliminary performances is presented

  6. Parasites as biological tags for the discrimination of marine fish stocks in Brazil: current status and perspectives

    Directory of Open Access Journals (Sweden)

    Iris Aparecida Soares

    2016-11-01

    Full Text Available ABSTRACT. Soares I.A. & Luque J.L. [Parasites as biological tags for the discrimination of marine fish stocks in Brazil: current status and perspectives.] Parasitos como marcadores biológicos para discriminação de estoques de peixes marinhos no Brasil: estado atual e perspectivas. Revista Brasileira de Medicina Veterinária, 38(supl. 3:99-113, 2016. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, BR 465 Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: luqueufrrj@gmail.com The global state of marine fisheries and its effects endanger the future of fishery resources, which may result in extinction of several species as well as threatening the overall integrity of the ecosystems. As the fish consumption growths, marine fishing and related market activities are stimulated increasing the incidence of threatened or exploited species. Aiming the future sustainability, fishery inventories need to be properly identified as a tool for implementation of more efficient policies on the management and conservation of the natural resources. Thus, by the high heterogeneity observed in the Atlantic coast of Brazil as well as the lack of related studies using this tool, the country represents great potential for the use of this technique, to improve our knowledge of local fishing resources. Therefore, the present study highlights the use of parasites as biological markers on identifying fish populations through robust statistical analysis, which represents an efficient and low cost approach and the lack of similar studies in Brazil showing the need of more research efforts on this subject in Brazil.

  7. Multiple Interests of Users in Collaborative Tagging Systems

    Science.gov (United States)

    Au Yeung, Ching-Man; Gibbins, Nicholas; Shadbolt, Nigel

    Performance of recommender systems depends on whether the user profiles contain accurate information about the interests of the users, and this in turn relies on whether enough information about their interests can be collected. Collaborative tagging systems allow users to use their own words to describe their favourite resources, resulting in some user-generated categorisation schemes commonly known as folksonomies. Folksonomies thus contain rich information about the interests of the users, which can be used to support various recommender systems. Our analysis of the folksonomy in Delicious reveals that the interests of a single user can be very diverse. Traditional methods for representing interests of users are usually not able to reflect such diversity. We propose a method to construct user profiles of multiple interests from folksonomies based on a network clustering technique. Our evaluation shows that the proposed method is able to generate user profiles which reflect the diversity of user interests and can be used as a basis of providing more focused recommendation to the users.

  8. Tag-n-Track system for situation awareness for MOUTs

    Science.gov (United States)

    Kumar, Rakesh; Aggarwal, Manoj; Germano, Thomas E.; Zhao, Tao; Fontana, Robert; Bushika, Martin

    2006-05-01

    In order to train war fighters for urban warfare, live exercises are held at various Military Operations on Urban Terrain (MOUT) facilities. Commanders need to have situation awareness (SA) of the entire mock battlefield, and also the individual actions of the various war fighters. The commanders must be able to provide instant feedback and play through different actions and 'what-if' scenarios with the war fighters. The war fighters in their turn should be able to review their actions and rehearse different maneuvers. In this paper, we describe the technologies behind a prototype training system, which tracks war fighters around an urban site using a combination of ultra-wideband (UWB) Radio Frequency Identification (RFID) and smart video based tracking. The system is able to: (1) Tag each individual with an unique ID using an RFID system, (2) Track and locate individuals within the domain of interest, (3) Associate IDs with visual appearance derived from live videos, (4) Visualize movement and actions of individuals within the context of a 3D model, and (5) Store and review activities with (x,y,ID) information associated with each individual. Dynamic acquisition and recording of the precise location of individual troops and units during training greatly aids the analysis of the training sessions allowing improved review, critique and instruction.

  9. Evaluation and implementation of QR Code Identity Tag system for Healthcare in Turkey

    OpenAIRE

    Uzun, Vassilya; Bilgin, Sami

    2016-01-01

    For this study, we designed a QR Code Identity Tag system to integrate into the Turkish healthcare system. This system provides QR code-based medical identification alerts and an in-hospital patient identification system. Every member of the medical system is assigned a unique QR Code Tag; to facilitate medical identification alerts, the QR Code Identity Tag can be worn as a bracelet or necklace or carried as an ID card. Patients must always possess the QR Code Identity bracelets within hospi...

  10. Evaluation of visible implant elastomer tags in zebrafish (Danio rerio

    Directory of Open Access Journals (Sweden)

    Claudia Hohn

    2013-11-01

    The use of the visible implant elastomer (VIE tagging system in zebrafish (Danio rerio was examined. Two tag orientations (horizontal and vertical at the dorsal fin base were tested for tag retention, tag fragmentation and whether VIE tags affected growth and survival of juvenile zebrafish (1–4 month post hatch. Six tag locations (abdomen, anal fin base, caudal peduncle, dorsal fin base, pectoral fin base, isthmus and 5 tag colors (yellow, red, pink, orange, blue were evaluated for ease of VIE tag application and tag visibility in adult zebrafish. Long-term retention (1 year and multiple tagging sites (right and left of dorsal fin and pectoral fin base were examined in adult zebrafish. Lastly, survival of recombination activation gene 1−/− (rag1−/− zebrafish was evaluated after VIE tagging. The best tag location was the dorsal fin base, and the most visible tag color was pink. Growth rate of juvenile zebrafish was not affected by VIE tagging. Horizontal tagging is recommended in early stages of fish growth (1–2 months post hatch. VIE tags were retained for 1 year and tagging did not interfere with long-term growth and survival. There was no mortality associated with VIE tagging in rag1−/− zebrafish. The VIE tagging system is highly suitable for small-sized zebrafish. When familiar with the procedure, 120 adult zebrafish can be tagged in one hour. It does not increase mortality in adult zebrafish or interfere with growth in juvenile or adult zebrafish.

  11. Radio frequency tags systems to initiate system processing

    Science.gov (United States)

    Madsen, Harold O.; Madsen, David W.

    1994-09-01

    This paper describes the automatic identification technology which has been installed at Applied Magnetic Corp. MR fab. World class manufacturing requires technology exploitation. This system combines (1) FluoroTrac cassette and operator tracking, (2) CELLworks cell controller software tools, and (3) Auto-Soft Inc. software integration services. The combined system eliminates operator keystrokes and errors during normal processing within a semiconductor fab. The methods and benefits of this system are described.

  12. Comparing autonomous underwater vehicle (AUV) and vessel-based tracking performance for locating acoustically tagged fish

    OpenAIRE

    Eiler, John H.; Grothues, Thomas M.; Dobarro, Joseph A.; Masuda, Michele M.

    2013-01-01

    Autonomous underwater vehicles (AUV’s) are increasingly used to collect physical, chemical, and biological information in the marine environment. Recent efforts include merging AUV technology with acoustic telemetry to provide information on the distribution and movements of marine fish. We compared surface vessel and AUV tracking capabilities under rigorous conditions in coastal waters near Juneau, Alaska. Tracking surveys were conducted with a REMUS 100 AUV equipped with an integrated acous...

  13. External tagging does not affect the feeding behavior of a coral reef fish, Chaetodon vagabundus (Pisces: Chaetodontidae)

    KAUST Repository

    Berumen, Michael L.; Almany, Glenn R.

    2009-01-01

    and cost efficient. However, a key assumption is that neither the tagging procedure nor the presence of a tag negatively affects the individual. While this has been demonstrated for relatively coarse metrics such as growth and survival, few studies have

  14. Method of remote powering and detecting multiple UWB passive tags in an RFID system

    Science.gov (United States)

    Dowla, Farid U [Castro Valley, CA; Nekoogar, Faranak [San Ramon, CA; Benzel, David M [Livermore, CA; Dallum, Gregory E [Livermore, CA; Spiridon, Alex [Palo Alto, CA

    2012-05-29

    A new Radio Frequency Identification (RFID), tracking, powering apparatus/system and method using coded Ultra-wideband (UWB) signaling is introduced. The proposed hardware and techniques disclosed herein utilize a plurality of passive UWB transponders in a field of an RFID-radar system. The radar system itself enables multiple passive tags to be remotely powered (activated) at about the same time frame via predetermined frequency UWB pulsed formats. Once such tags are in an activated state, an UWB radar transmits specific "interrogating codes" to put predetermined tags in an awakened status. Such predetermined tags can then communicate by a unique "response code" so as to be detected by an UWB system using radar methods.

  15. Multi-use Passive RFID Sensor Tag System for NASA, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal will provide NASA with a family of UHF RFID sensor tags and system components supporting very reliable, robust, convenient and economical deployment...

  16. Evaluating the Cognitive Aspects of User Interaction with 2D Visual Tagging Systems

    Directory of Open Access Journals (Sweden)

    Samuel Olugbenga King

    2008-04-01

    Full Text Available There has been significant interest in thedevelopment and deployment of visual taggingapplications in recent times. But user perceptions aboutthe purpose and function of visual tagging systems havenot received much attention. This paper presents a userexperience study that investigates the cognitive modelsthat novice users have about interacting with visualtagging applications. The results of the study show thatalthough most users are unfamiliar with visual taggingtechnologies, they could accurately predict the purposeand mode of retrieval of data stored in visual tags. Thestudy concludes with suggestions on how to improve therecognition, ease of recall and design of visual tags.

  17. Temperature profiles collected by Commonwealth Scientific Industrial Research Organization (CSIRO) from Fish Tag data from the Coral Sea, Tasman Sea, and the Indian Oceans from 15 November 2008 to 26 July 2009 (NODC Accession 0067650)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from Fish Tag data from the biologging group at CSIRO, from the Coral Sea, Tasman Sea, and the Indian Oceans from 15 November...

  18. Monitoring System for Slope Stability under Rainfall by using MEMS Acceleration Sensor IC tags

    International Nuclear Information System (INIS)

    Murakami, S; Dairaku, A; Komine, H; Saito, O; Sakai, N; Isizawa, T; Maruyama, I

    2013-01-01

    Real-time warning system for slope failure under rainfall is available to disaster prevention and mitigation. Monitoring of multi-point and wireless measurements is effective because it is difficult to conclude the most dangerous part in a slope. The purpose of this study is to propose a method of monitoring system with multi-point and wireless measurements for a slope stability using MEMS acceleration sensor IC tags. MEMS acceleration sensor IC tag is an acceleration sensor microminiaturized by a technology of Micro Electro Mechanical Systems on board IC tag. Especially, low cost of the sensor will yield to the realization of the system. In order to investigate the applicability of the proposed system, a large-scale model test of artificial slope subjected to rainfall has been performed. MEMS acceleration sensor IC tags has been located on the slope and ground acceleration caused by forced vibration has been measured until the model slope collapses. The experimental results show that the MEMS acceleration sensor IC tag is comfortably available under rainfall, the characteristics of ground accelerations varies with changing the condition of the slope subjected to rainfall, and the proposed method can be applied to a real-time monitoring system for slope failure under rainfall.

  19. Simulator for testing hardware and software of the office system with RFID tags

    Directory of Open Access Journals (Sweden)

    Nowicki Tadeusz

    2017-01-01

    Full Text Available This paper presents the method for examining the properties of the RFID-tagged document management system. The system is composed of computers, where the software for supporting processes of the RFID-tagged documents was installed. Furthermore, the system cooperates with many other elements of the office (cabinets, sluices, copiers, try rider, end so one. The examination of the properties of the RFID-tagged document management system is, in this case, complex due to the number of a possible examination scenarios. The simulator method for examining the system properties was design and implemented. It allows to conduct the examination of the properties in a short period of time for numerous testing scenarios.

  20. Software simulator for property investigation of document management system with RFID tags

    Directory of Open Access Journals (Sweden)

    Kiedrowicz Maciej

    2016-01-01

    Full Text Available The study outlines the method for examining the properties of the RFID-tagged document management system. The system is composed of computers, where the software for supporting processes of the RFID-tagged doc-uments was installed. Furthermore, the system cooperates with many other elements of the secret office (cabinets, sluices, photocopiers, desks. The examination of the properties of the RFID-tagged document management system is, in this case, complex due to the number of a possible examination scenarios. The simulation method for examining the system properties was proposed. It allows to conduct the examination of the properties in a short period of time for numerous testing scenarios.

  1. A Secure RFID Tag Authentication Protocol with Privacy Preserving in Telecare Medicine Information System.

    Science.gov (United States)

    Li, Chun-Ta; Weng, Chi-Yao; Lee, Cheng-Chi

    2015-08-01

    Radio Frequency Identification (RFID) based solutions are widely used for providing many healthcare applications include patient monitoring, object traceability, drug administration system and telecare medicine information system (TMIS) etc. In order to reduce malpractices and ensure patient privacy, in 2015, Srivastava et al. proposed a hash based RFID tag authentication protocol in TMIS. Their protocol uses lightweight hash operation and synchronized secret value shared between back-end server and tag, which is more secure and efficient than other related RFID authentication protocols. Unfortunately, in this paper, we demonstrate that Srivastava et al.'s tag authentication protocol has a serious security problem in that an adversary may use the stolen/lost reader to connect to the medical back-end server that store information associated with tagged objects and this privacy damage causing the adversary could reveal medical data obtained from stolen/lost readers in a malicious way. Therefore, we propose a secure and efficient RFID tag authentication protocol to overcome security flaws and improve the system efficiency. Compared with Srivastava et al.'s protocol, the proposed protocol not only inherits the advantages of Srivastava et al.'s authentication protocol for TMIS but also provides better security with high system efficiency.

  2. The photon tagging system of the PHOENICS-experiment at ELSA

    Energy Technology Data Exchange (ETDEWEB)

    Detemple, P.; Althoff, K.H.; Anton, G.; Arends, J.; Bock, A.; Breuer, M.; Buechler, K.; Noeldeke, G.; Serwazi, M.; Schneider, W.; Urban, D.; Zucht, B. (Physikalisches Inst. der Univ. Bonn (Germany))

    1992-10-01

    This paper describes the setup and the first test of the tagging systems of the PHOENICS experiment at the electron stretcher ELSA in Bonn. The system covers the energy range k = 200-950 MeV simultaneously with an energy resolution of 5-10 MeV (FWHM). The tagging system is in operation since early 1990 and has been successfully used in first measurements of [gamma]p [yields] [pi][sup +]n and for [eta]-production from proton and deuterium targets. (orig.).

  3. The photon tagging system of the PHOENICS-experiment at ELSA

    International Nuclear Information System (INIS)

    Detemple, P.; Althoff, K.H.; Anton, G.; Arends, J.; Bock, A.; Breuer, M.; Buechler, K.; Noeldeke, G.; Serwazi, M.; Schneider, W.; Urban, D.; Zucht, B.

    1992-01-01

    This paper describes the setup and the first test of the tagging systems of the PHOENICS experiment at the electron stretcher ELSA in Bonn. The system covers the energy range k = 200-950 MeV simultaneously with an energy resolution of 5-10 MeV (FWHM). The tagging system is in operation since early 1990 and has been successfully used in first measurements of γp → π + n and for η-production from proton and deuterium targets. (orig.)

  4. Evaluation and implementation of QR Code Identity Tag system for Healthcare in Turkey.

    Science.gov (United States)

    Uzun, Vassilya; Bilgin, Sami

    2016-01-01

    For this study, we designed a QR Code Identity Tag system to integrate into the Turkish healthcare system. This system provides QR code-based medical identification alerts and an in-hospital patient identification system. Every member of the medical system is assigned a unique QR Code Tag; to facilitate medical identification alerts, the QR Code Identity Tag can be worn as a bracelet or necklace or carried as an ID card. Patients must always possess the QR Code Identity bracelets within hospital grounds. These QR code bracelets link to the QR Code Identity website, where detailed information is stored; a smartphone or standalone QR code scanner can be used to scan the code. The design of this system allows authorized personnel (e.g., paramedics, firefighters, or police) to access more detailed patient information than the average smartphone user: emergency service professionals are authorized to access patient medical histories to improve the accuracy of medical treatment. In Istanbul, we tested the self-designed system with 174 participants. To analyze the QR Code Identity Tag system's usability, the participants completed the System Usability Scale questionnaire after using the system.

  5. A UHF RFID system with on-chip-antenna tag for short range communication

    International Nuclear Information System (INIS)

    Peng Qi; Zhang Chun; Zhao Xijin; Wang Zhihua

    2015-01-01

    A UHF RF identification system based on the 0.18 μm CMOS process has been developed for short range and harsh size requirement applications, which is composed of a fully integrated tag and a special reader. The whole tag chip with the antenna takes up an area of 0.36 mm 2 , which is smaller than other reported tags with an on-chip antenna (OCA) using the standard CMOS process. A self-defined protocol is proposed to reduce the power consumption, and minimize the size of the tag. The specialized SOC reader system consists of the RF transceiver, digital baseband, MCU and host interface. Its power consumption is about 500 mW. Measurement results show that the system's reading range is 2 mm with 20 dBm reader output power. With an inductive antenna printed on a paper substrate around the OCA tag, the reading range can be extended from several centimeters to meters, depending on the shape and size of the inductive antenna. (paper)

  6. A Security Mechanism for library management system using low cost RFID tags

    Directory of Open Access Journals (Sweden)

    V. Nagalakshmi

    2007-02-01

    Full Text Available Radio Frequency Identification (RFID systems will become pervasive in our daily lives due to their low cost and easy to use characteristics[1]. This paper presents a methodology of using low cost RFID tags for a library management system to protect the books from unauthorized capturing and usage. Every object to be identified in the RFID system is physically labeled with a tag. In the proposed method a book or a magazine or a CD is identified with the RFID tag. Whenever a book is issued to any user of the library, the RFID reader will capture the information of that book and compares it with the related information of the book in the Online Public Access Catalogue (OPAC and issues the book to the user depending on his identity in the database. If any unauthorized person intends to take the book the RFID reader will immediately respond to that and make sure that it was not issued. If any body tries to remove the tag it can be traced with the help of location device. Keywords: RFID, OPAC, Unauthorized user, tag, Reader.

  7. 50 CFR 697.19 - Trap limits and trap tag requirements for vessels fishing with lobster traps.

    Science.gov (United States)

    2010-10-01

    ... vessels fishing with lobster traps. 697.19 Section 697.19 Wildlife and Fisheries FISHERY CONSERVATION AND... requirements for vessels fishing with lobster traps. (a) Trap limits for vessels fishing or authorized to fish... management area designation certificate or valid limited access American lobster permit specifying one or...

  8. Performance of a tagged neutron inspection system (TNIS) based on portable sealed generators

    International Nuclear Information System (INIS)

    Nebbia, G.; Pesente, S.; Lunardon, M.; Viesti, G.; LeTourneur, P.; Heuveline, F.; Mangeard, M.; Tcheng, C.

    2004-01-01

    A portable sealed neutron generator has been modified to produce 14MeV tagged neutron beams with an embedded YAP:Ce scintillation detector. The system has been tested by detecting the coincident gamma-rays produced in the irradiation of a graphite sample by means of a standard NaI(Tl) scintillator. Time resolution of about δt=4-5ns (FWHM) has been measured. The sealed neutron tube has been operated up to 10 7 neutron/s. Possible applications in non-destructive assays and future developments of the Tagged Neutron Inspection System concept are discussed

  9. CROSS-DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY: Diffusion-Based Recommendation in Collaborative Tagging Systems

    Science.gov (United States)

    Shang, Ming-Sheng; Zhang, Zi-Ke

    2009-11-01

    Recently, collaborative tagging systems have attracted more and more attention and have been widely applied in web systems. Tags provide highly abstracted information about personal preferences and item content, and therefore have the potential to help in improving better personalized recommendations. We propose a diffusion-based recommendation algorithm considering the personal vocabulary and evaluate it in a real-world dataset: Del.icio.us. Experimental results demonstrate that the usage of tag information can significantly improve the accuracy of personalized recommendations.

  10. Expert system for identification of simultaneous and sequential reactor fuel failures with gas tagging

    Science.gov (United States)

    Gross, Kenny C.

    1994-01-01

    Failure of a fuel element in a nuclear reactor core is determined by a gas tagging failure detection system and method. Failures are catalogued and characterized after the event so that samples of the reactor's cover gas are taken at regular intervals and analyzed by mass spectroscopy. Employing a first set of systematic heuristic rules which are applied in a transformed node space allows the number of node combinations which must be processed within a barycentric algorithm to be substantially reduced. A second set of heuristic rules treats the tag nodes of the most recent one or two leakers as "background" gases, further reducing the number of trial node combinations. Lastly, a "fuzzy" set theory formalism minimizes experimental uncertainties in the identification of the most likely volumes of tag gases. This approach allows for the identification of virtually any number of sequential leaks and up to five simultaneous gas leaks from fuel elements.

  11. Expert system for identification of simultaneous and sequential reactor fuel failures with gas tagging

    International Nuclear Information System (INIS)

    Gross, K.C.

    1994-01-01

    Failure of a fuel element in a nuclear reactor core is determined by a gas tagging failure detection system and method. Failures are catalogued and characterized after the event so that samples of the reactor's cover gas are taken at regular intervals and analyzed by mass spectroscopy. Employing a first set of systematic heuristic rules which are applied in a transformed node space allows the number of node combinations which must be processed within a barycentric algorithm to be substantially reduced. A second set of heuristic rules treats the tag nodes of the most recent one or two leakers as ''background'' gases, further reducing the number of trial node combinations. Lastly, a ''fuzzy'' set theory formalism minimizes experimental uncertainties in the identification of the most likely volumes of tag gases. This approach allows for the identification of virtually any number of sequential leaks and up to five simultaneous gas leaks from fuel elements. 14 figs

  12. Invisible marking system by extreme ultraviolet radiation: the new frontier for anti-counterfeiting tags

    International Nuclear Information System (INIS)

    Lazzaro, P. Di; Bollanti, S.; Flora, F.; Mezi, L.; Murra, D.; Torre, A.; Bonfigli, F.; Montereali, R.M.; Vincenti, M.A.

    2016-01-01

    We present a marking technology which uses extreme ultraviolet radiation to write invisible patterns on tags based on alkali fluoride thin films. The shape of the pattern is pre-determined by a mask (in the case of contact lithography) or by a suitable mirror (projection lithography). Tags marked using this method offer a much better protection against fakes than currently available anti-counterfeiting techniques. The complexity and cost of this technology can be tailored to the value of the good to be protected, leaving, on the other hand, the specific reading technique straightforward. So far, we have exploited our invisible marking to tag artworks, identity cards, electrical components, and containers of radioactive wastes. Advantages and limits of this technology are discussed in comparison with the anti-counterfeiting systems available in the market.

  13. Extraction of peptide tagged cutinase in detergent-based aqueous two-phase systems

    NARCIS (Netherlands)

    Rodenbrock, A.; Selber, K.; Egmond, M.R.; Kula, M.-R.

    2010-01-01

    Detergent-based aqueous two-phase systems have the advantage to require only one auxiliary chemical to induce phase separation above the cloud point. In a systematic study the efficiency of tryptophan-rich peptide tags was investigated to enhance the partitioning of an enzyme to the detergent-rich

  14. Coded-Wire Tag Expansion Factors for Chinook Salmon Carcass Surveys in California: Estimating the Numbers and Proportions of Hatchery-Origin Fish

    Directory of Open Access Journals (Sweden)

    Michael S. Mohr

    2013-12-01

    Full Text Available Recovery of fish with adipose fin clips (adc and coded-wire tags (cwt in escapement surveys allows calculation of expansion factors used in estimation of the total number of fish from each adc,cwt release group, allowing escapement to be resolved by age and stock of origin. Expanded recoveries are used to derive important estimates such as the total number and proportion of hatchery-origin fish present. The standard estimation scheme assumes accurate visual classification of adc status, which can be problematic for decomposing carcasses. Failure to account for this potential misclassification can lead to significant estimation bias. We reviewed sample expansion factors used for the California Central Valley Chinook salmon 2010 carcass surveys in this context. For upper Sacramento River fall-run and late fall-run carcass surveys, the estimated proportions of adc,cwt fish for fresh and non-fresh carcasses differed substantially, likely from the under-recognition of adc fish in non-fresh carcasses. The resulting estimated proportions of hatchery-origin fish in the upper Sacramento River fall-run and late fall-run carcass surveys were 2.33 to 2.89 times higher if only fresh carcasses are considered. Similar biases can be avoided by consideration of only fresh carcasses for which determination of adc status is relatively straightforward; however, restricting the analysis entirely to fresh carcasses may limit precision because of reduced sample size, and is only possible if protocols for sampling and recording data ensure that the sample data and results for fresh carcasses can be extracted. Thus we recommend sampling protocols that are clearly documented and separately track fresh versus non-fresh carcasses, either collecting only definitively adc fish or that carefully track non-fresh carcasses that are definitively adc versus those that are possibly adc. This would allow judicious use of non-fresh carcass data when sample sizes are otherwise

  15. Billfish Tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The SWFSC's constituent-based Billfish Tagging Program began in 1963 and since that time has provided conventional spaghetti type tags and tagging supplies to...

  16. Tag questions Tag questions

    Directory of Open Access Journals (Sweden)

    David Brazil

    2008-04-01

    Full Text Available The so-called 'tag' structures of English have received a lot of attention in language teaching programmes, attention that is not hard to justify when one considers the problems and anxiety they can occasion for many foreign learners. Most teachers one speaks to seem fairly willing to agree, however, that traditional treatments of the topic leave much to be desired. It happens, also, that, when considered collectively, the tags and some related phenomena have a special heoretical interest. For they constitute a field in which it seems essential to bring together insights that derive from the study of several aspects of linguistic organisation, aspects which in some recent work have been held to need distinctive kinds of descriptive category to handle. Traditional treatments have found it necessary to recognise different syntactic types (e.g. 'same polarity' and 'reversed polarity' tags and ifferent intonational treatments ("falling'and 'rising' tag; while the way the communicative significance of the various permutations is described normally requires reference to the expectations they signal regarding the immediately following behaviour of the other party (in the common phrase, 'What kind of answer they expect'. This last consideration places the matter squarely in the arena of recent work on the analysis of interactive discourse. The so-called 'tag' structures of English have received a lot of attention in language teaching programmes, attention that is not hard to justify when one considers the problems and anxiety they can occasion for many foreign learners. Most teachers one speaks to seem fairly willing to agree, however, that traditional treatments of the topic leave much to be desired. It happens, also, that, when considered collectively, the tags and some related phenomena have a special heoretical interest. For they constitute a field in which it seems essential to bring together insights that derive from the study of several aspects

  17. Ensemble system for Part-of-Speech tagging

    OpenAIRE

    Dell'Orletta, Felice

    2009-01-01

    The paper contains a description of the Felice-POS-Tagger and of its performance in Evalita 2009. Felice-POS-Tagger is an ensemble system that combines six different POS taggers. When evaluated on the official test set, the ensemble system outperforms each of the single tagger components and achieves the highest accuracy score in Evalita 2009 POS Closed Task. It is shown rst that the errors made from the dierent taggers are complementary, and then how to use this complementary behavior to the...

  18. User and Document Group Approach of Clustering in Tagging Systems

    DEFF Research Database (Denmark)

    Pan, Rong; Xu, Guandong; Dolog, Peter

    2010-01-01

    In this paper, we propose a spectral clustering approach for users and documents group modeling in order to capture the common preference and relatedness of users and documents, and to reduce the time complexity of similarity calculations. In experiments, we investigate the selection of the optim...... amount of clusters. We also show a reduction of the time consuming in calculating the similarity for the recommender systems by selecting a centroid first, and then compare the inside item on behalf of each group....

  19. Extracting Tag Hierarchies

    Science.gov (United States)

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2013-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the “flat” organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of search

  20. Extracting tag hierarchies.

    Directory of Open Access Journals (Sweden)

    Gergely Tibély

    Full Text Available Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the "flat" organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of

  1. Extracting tag hierarchies.

    Science.gov (United States)

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2013-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the "flat" organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of search. Moreover

  2. The role of tag suggestions in folksonomies

    NARCIS (Netherlands)

    Bollen, D.G.F.M.; Halpin, H.

    2009-01-01

    Most tagging systems support the user in the tag selection process by providing tag suggestions, or recommendations, based on a popularity measurement of tags other users provided when tagging the same resource. The majority of theories and mathematical models of tagging found in the literature

  3. PASTE: patient-centered SMS text tagging in a medication management system.

    Science.gov (United States)

    Stenner, Shane P; Johnson, Kevin B; Denny, Joshua C

    2012-01-01

    To evaluate the performance of a system that extracts medication information and administration-related actions from patient short message service (SMS) messages. Mobile technologies provide a platform for electronic patient-centered medication management. MyMediHealth (MMH) is a medication management system that includes a medication scheduler, a medication administration record, and a reminder engine that sends text messages to cell phones. The object of this work was to extend MMH to allow two-way interaction using mobile phone-based SMS technology. Unprompted text-message communication with patients using natural language could engage patients in their healthcare, but presents unique natural language processing challenges. The authors developed a new functional component of MMH, the Patient-centered Automated SMS Tagging Engine (PASTE). The PASTE web service uses natural language processing methods, custom lexicons, and existing knowledge sources to extract and tag medication information from patient text messages. A pilot evaluation of PASTE was completed using 130 medication messages anonymously submitted by 16 volunteers via a website. System output was compared with manually tagged messages. Verified medication names, medication terms, and action terms reached high F-measures of 91.3%, 94.7%, and 90.4%, respectively. The overall medication name F-measure was 79.8%, and the medication action term F-measure was 90%. Other studies have demonstrated systems that successfully extract medication information from clinical documents using semantic tagging, regular expression-based approaches, or a combination of both approaches. This evaluation demonstrates the feasibility of extracting medication information from patient-generated medication messages.

  4. TOPAS 2 - a high-resolution tagging system at the Bonn SAPHIR detector

    International Nuclear Information System (INIS)

    Rappenecker, G.

    1989-02-01

    For the SAPHIR-arrangement in Bonn a high resolving tagging system has been developed achieving an energy resolution of 2 MeV, covering the range of (0.94-0.34) E 0 photon energy (1.0 GeV 0 2 , ArCH 4 and ArC 2 H 6 in concern of performance, clustersize and coincidence width. (orig.)

  5. Alien fish species in reservoir systems in Turkey: a review

    OpenAIRE

    Deniz Innal

    2012-01-01

    Turkey’s natural river systems have been anthropogenically altered in the past century. Native fish communities of river systems have comeunder increasing pressure from water engineering projects, pollution, overfishing and the movements of alien fish species. Introduction ofalien fishes is one of the main threats to the survival and genetic integrity of native fishes around the world. In Turkey, alien freshwater fish are continuing to increase in number of species, abundance, and distributio...

  6. Invasive alien freshwater fishes in the Wilderness Lakes System, a ...

    African Journals Online (AJOL)

    Invasive alien freshwater fishes in the Wilderness Lakes System, a wetland of international importance in the Western Cape Province, South Africa. ... A total of 87 893 fish comprising 16 species were caught. In addition to confirming the ... Key words: freshwater fish, invasive alien fishes, estuary, RAMSAR site, diversity.

  7. Fish pigmentation and the melanocortin system.

    Science.gov (United States)

    Cal, Laura; Suarez-Bregua, Paula; Cerdá-Reverter, José Miguel; Braasch, Ingo; Rotllant, Josep

    2017-09-01

    The melanocortin system is a complex neuroendocrine signaling mechanism involved in numerous physiological processes in vertebrates, including pigmentation, steroidogenesis and metabolic control. This review focuses at one of its most fascinating function in fish, its regulatory role in the control of pigmentation, in which the melanocortin 1 receptor (Mc1r), its agonist α-melanocyte stimulating hormone (α-Msh), and the endogenous antagonist agouti signaling protein (Asip1) are the main players. Functional control of Mc1r, which is highly expressed in fish skin and whose activation stimulates melanin production and melanosome dispersion in fish melanophores, is considered a key mechanism for vertebrate pigment phenotypes. The α-Msh peptide, the most documented Mc1r agonist involved in pigmentation, is produced in the pituitary gland, activating melanin synthesis by binding to Mc1r in fish melanophores. Finally, Asip1 is the putative factor for establishing the evolutionarily conserved dorso-ventral pigment pattern found across vertebrates. However, we are just starting to understand how other melanocortin system components are acting in this complex regulatory network. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. A comparative evaluation of three methods used to tag South African ...

    African Journals Online (AJOL)

    Tagging effects and loss rates of 60 Roman Chrysoblephus laticeps tagged with dart tags with barbs (D-tags), T-bar filaments (T-tags) and visible implant fluorescent elastomer (VIFE) tags were investigated. The fish were tagged and monitored in a controlled tank experiment over a period of 198 days. Application technique ...

  9. An orange fluorescent protein tagging system for real-time pollen tracking.

    Science.gov (United States)

    Rice, J Hollis; Millwood, Reginald J; Mundell, Richard E; Chambers, Orlando D; Abercrombie, Laura L; Davies, H Maelor; Stewart, C Neal

    2013-09-27

    Monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum × Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification. We report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a tagged Nicotiana hybrid that could be used for the incidence of gene flow. Nicotiana tabacum 'TN 90' and Nicotiana glauca were successfully transformed via Agrobacterium tumefaciens to express the orange fluorescent protein gene, tdTomato-ER, in pollen and a green fluorescent protein gene, mgfp5-er, was expressed in vegetative structures of the plant. Hybrids were created that utilized the fluorescent proteins as a research tool for monitoring pollen movement and gene flow. Manual greenhouse crosses were used to assess hybrid sexual compatibility with N. tabacum, resulting in seed formation from hybrid pollination in 2% of crosses, which yielded non-viable seed. Pollen transfer to the hybrid formed seed in 19% of crosses and 10 out of 12 viable progeny showed GFP expression. The orange fluorescent protein is visible when expressed in the pollen of N. glauca, N. tabacum, and the Nicotiana hybrid, although hybrid pollen did not appear as bright as the parent lines. The hybrid plants, which show limited ability to outcross, could provide bioconfinement with the benefit of detectable pollen using this system. Fluorescent protein-tagging could be a valuable tool for breeding and in vivo ecological monitoring.

  10. Alien fish species in reservoir systems in Turkey: a review

    Directory of Open Access Journals (Sweden)

    Deniz Innal

    2012-12-01

    Full Text Available Turkey’s natural river systems have been anthropogenically altered in the past century. Native fish communities of river systems have comeunder increasing pressure from water engineering projects, pollution, overfishing and the movements of alien fish species. Introduction ofalien fishes is one of the main threats to the survival and genetic integrity of native fishes around the world. In Turkey, alien freshwater fish are continuing to increase in number of species, abundance, and distribution. The present paper reviews fish stocking studies in Turkey’s reservoirs.

  11. Influence of depth, time and human activity on detection rate of acoustic tags: a case study on two fish farms.

    Science.gov (United States)

    Otterå, H; Skilbrei, O T

    2016-03-01

    The detection rates of stationary acoustic transmitters deployed at three depths on two Atlantic salmon Salmo salar cage farms for c. 2 months were investigated. Deployment depth, time of day, day of the year and specific incidences at the farm substantially affected the rate of signal detection by the receiver. © 2016 The Authors. Journal of Fish Biology published by John Wiley & Sons Ltd on behalf of The Fisheries Society of the British Isles.

  12. How many fish in a tank? Constructing an automated fish counting system by using PTV analysis

    Science.gov (United States)

    Abe, S.; Takagi, T.; Takehara, K.; Kimura, N.; Hiraishi, T.; Komeyama, K.; Torisawa, S.; Asaumi, S.

    2017-02-01

    Because escape from a net cage and mortality are constant problems in fish farming, health control and management of facilities are important in aquaculture. In particular, the development of an accurate fish counting system has been strongly desired for the Pacific Bluefin tuna farming industry owing to the high market value of these fish. The current fish counting method, which involves human counting, results in poor accuracy; moreover, the method is cumbersome because the aquaculture net cage is so large that fish can only be counted when they move to another net cage. Therefore, we have developed an automated fish counting system by applying particle tracking velocimetry (PTV) analysis to a shoal of swimming fish inside a net cage. In essence, we treated the swimming fish as tracer particles and estimated the number of fish by analyzing the corresponding motion vectors. The proposed fish counting system comprises two main components: image processing and motion analysis, where the image-processing component abstracts the foreground and the motion analysis component traces the individual's motion. In this study, we developed a Region Extraction and Centroid Computation (RECC) method and a Kalman filter and Chi-square (KC) test for the two main components. To evaluate the efficiency of our method, we constructed a closed system, placed an underwater video camera with a spherical curved lens at the bottom of the tank, and recorded a 360° view of a swimming school of Japanese rice fish (Oryzias latipes). Our study showed that almost all fish could be abstracted by the RECC method and the motion vectors could be calculated by the KC test. The recognition rate was approximately 90% when more than 180 individuals were observed within the frame of the video camera. These results suggest that the presented method has potential application as a fish counting system for industrial aquaculture.

  13. PTAGIS - Development of Large PIT-Tag Antennas to Estimate Migration Timing and Survival for Adult Salmonids near Pile Dikes in the Columbia River Estuary

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — We continued research and development of a passive PIT-tag detection system along a pile dike in the estuary (rkm 70). Target fish for this system are returning...

  14. Compliant lightweight non-invasive standalone “Marine Skin” tagging system

    KAUST Repository

    Nassar, Joanna M.

    2018-04-16

    Current marine research primarily depends on weighty and invasive sensory equipment and telemetric network to understand the marine environment, including the diverse fauna it contains, as a function of animal behavior and size, as well as equipment longevity. To match animal morphology and activity within the surrounding marine environment, here we show a physically flexible and stretchable skin-like and waterproof autonomous multifunctional system, integrating Bluetooth, memory chip, and high performance physical sensors. The sensory tag is mounted on a swimming crab (Portunus pelagicus) and is capable of continuous logging of depth, temperature, and salinity within the harsh ocean environment. The fully packaged, ultra-lightweight (<2.4 g in water), and compliant “Marine Skin” system does not have any wired connection enabling safe and weightless cutting-edge approach to monitor and assess marine life and the ecosystem’s health to support conservation and management of marine ecosystems.

  15. Factors Affecting Detection Probability of Acoustic Tags in Coral Reefs

    KAUST Repository

    Bermudez, Edgar F.

    2012-01-01

    of the transmitter detection range and the detection probability. A one-month range test of a coded telemetric system was conducted prior to a large-scale tagging project investigating the movement of approximately 400 fishes from 30 species on offshore coral reefs

  16. Passage Distribution and Federal Columbia River Power System Survival for Steelhead Kelts Tagged Above and at Lower Granite Dam, Year 2

    Energy Technology Data Exchange (ETDEWEB)

    Colotelo, Alison HA; Harnish, Ryan A.; Jones, Bryan W.; Hanson, Amanda C.; Trott, Donna M.; Greiner, Michael J.; McMichael, Geoffrey A.; Ham, Kenneth D.; Deng, Zhiqun; Brown, Richard S.; Weiland, Mark A.; Li, X.; Fu, Tao

    2014-03-28

    Steelhead (Oncorhynchus mykiss) populations have declined throughout their range in the last century and many populations, including those of the Snake River Basin are listed under the Endangered Species Act of 1973. The reasons for their decline are many and complex, but include habitat loss and degradation, overharvesting, and dam construction. The 2008 Biological Opinion calls for an increase in the abundance of female steelhead through an increase in iteroparity (i.e., repeat spawning) and this can be realized through a combination of reconditioning and in-river survival of migrating kelts. The goal of this study is to provide the data necessary to inform fisheries managers and dam operators of Snake River kelt migration patterns, survival, and routes of dam passage. Steelhead kelts (n = 487) were captured and implanted with acoustic transmitters and passive integrated transponder (PIT)-tags at the Lower Granite Dam (LGR) Juvenile Fish Facility and at weirs located in tributaries of the Snake and Clearwater rivers upstream of LGR. Kelts were monitored as they moved downstream through the Federal Columbia River Power System (FCRPS) by 15 autonomous and 3 cabled acoustic receiver arrays. Cabled receiver arrays deployed on the dam faces allowed for three-dimensional tracking of fish as they approached the dam face and were used to determine the route of dam passage. Overall, 27.3% of the kelts tagged in this study successfully migrated to Martin Bluff (rkm 126, as measured from the mouth of the Columbia River), which is located downstream of all FCRPS dams. Within individual river reaches, survival per kilometer estimates ranged from 0.958 to 0.999; the lowest estimates were observed in the immediate forebay of FCRPS dams. Steelhead kelts tagged in this study passed over the spillway routes (spillway weirs, traditional spill bays) in greater proportions and survived at higher rates compared to the few fish passed through powerhouse routes (turbines and juvenile

  17. Passage Distribution and Federal Columbia River Power System Survival for Steelhead Kelts Tagged Above and at Lower Granite Dam, Year 2

    Energy Technology Data Exchange (ETDEWEB)

    Colotelo, Alison H.A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Harnish, Ryan A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Jones, Bryan W. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hanson, Amanda C. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Trott, Donna M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Greiner, Michael J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mcmichael, Geoffrey A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ham, Kenneth D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Deng, Zhiqun [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Brown, Richard S. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Weiland, Mark A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Li, Xinya [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Fu, Tao [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2014-12-15

    Steelhead (Oncorhynchus mykiss) populations have declined throughout their range in the last century and many populations, including those of the Snake River Basin are listed under the Endangered Species Act of 1973. The reasons for their decline are many and complex, but include habitat loss and degradation, overharvesting, and dam construction. The 2008 Biological Opinion calls for an increase in the abundance of female steelhead through an increase in iteroparity (i.e., repeat spawning) and this can be realized through a combination of reconditioning and in-river survival of migrating kelts. The goal of this study is to provide the data necessary to inform fisheries managers and dam operators of Snake River kelt migration patterns, survival, and routes of dam passage. Steelhead kelts (n = 487) were captured and implanted with acoustic transmitters and passive integrated transponder (PIT)-tags at the Lower Granite Dam (LGR) Juvenile Fish Facility and at weirs located in tributaries of the Snake and Clearwater rivers upstream of LGR. Kelts were monitored as they moved downstream through the Federal Columbia River Power System (FCRPS) by 15 autonomous and 3 cabled acoustic receiver arrays. Cabled receiver arrays deployed on the dam faces allowed for three-dimensional tracking of fish as they approached the dam face and were used to determine the route of dam passage. Overall, 27.3% of the kelts tagged in this study successfully migrated to Martin Bluff (rkm 126, as measured from the mouth of the Columbia River), which is located downstream of all FCRPS dams. Within individual river reaches, survival per kilometer estimates ranged from 0.958 to 0.999; the lowest estimates were observed in the immediate forebay of FCRPS dams. Steelhead kelts tagged in this study passed over the spillway routes (spillway weirs, traditional spill bays) in greater proportions and survived at higher rates compared to the few fish passed through powerhouse routes (turbines and juvenile

  18. The Mucosal Immune System of Teleost Fish

    Directory of Open Access Journals (Sweden)

    Irene Salinas

    2015-08-01

    Full Text Available Teleost fish possess an adaptive immune system associated with each of their mucosal body surfaces. Evidence obtained from mucosal vaccination and mucosal infection studies reveal that adaptive immune responses take place at the different mucosal surfaces of teleost. The main mucosa-associated lymphoid tissues (MALT of teleosts are the gut-associated lymphoid tissue (GALT, skin-associated lymphoid tissue (SALT, the gill-associated lymphoid tissue (GIALT and the recently discovered nasopharynx-associated lymphoid tissue (NALT. Teleost MALT includes diffuse B cells and T cells with specific phenotypes different from their systemic counterparts that have co-evolved to defend the microbe-rich mucosal environment. Both B and T cells respond to mucosal infection or vaccination. Specific antibody responses can be measured in the gills, gut and skin mucosal secretions of teleost fish following mucosal infection or vaccination. Rainbow trout studies have shown that IgT antibodies and IgT+ B cells are the predominant B cell subset in all MALT and respond in a compartmentalized manner to mucosal infection. Our current knowledge on adaptive immunity in teleosts is limited compared to the mammalian literature. New research tools and in vivo models are currently being developed in order to help reveal the great intricacy of teleost mucosal adaptive immunity and help improve mucosal vaccination protocols for use in aquaculture.

  19. Ontologies and tag-statistics

    Science.gov (United States)

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2012-05-01

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of

  20. Ontologies and tag-statistics

    International Nuclear Information System (INIS)

    Tibély, Gergely; Vicsek, Tamás; Pollner, Péter; Palla, Gergely

    2012-01-01

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of

  1. Radio Tracking Fish with Small Unmanned Aircraft Systems (sUAS).

    Science.gov (United States)

    Dahlgren, R. P.; Anderson, K. R.; Hanson, L.; Pinsker, E. A.; Jonsson, J.; Chapman, D. C.; Witten, D. M.; O'Connor, K. A.

    2017-12-01

    Tracking radio tagged fish by boat or on foot in riverine systems is difficult and time consuming, particularly in large braided island complexes, shallow wetlands, and rocky reaches. Invasive Asian carp are commonly found in these hard to reach areas, but their near-surface feeding behavior makes radio tracking possible. To identify new methods of fish tracking that could same time and money, this study tested the feasibility of tracking Asian carp with Small Unmanned Aerial Systems (sUAS) in areas generally inaccessible to traditional tracking equipment. The U.S. Geological Survey worked with NanoElectromagnetics LLC and WWR Development to create and integrate a lightweight custom radio receiver, directional antenna, and accompanying software into a sUAS platform. The receiver includes independent GPS, software defined radio, and compass. The NASA Ames Research Center (ARC) completed payload integration, electromagnetic-interference and airworthiness testing, and provided a DJI Matrice 600 sUAS for this study. Additionally, ARC provided subject matter experts, airworthiness and flight readiness evaluation, and flight test facilities during preparation; and a pilot, range safety officer, and aircraft engineer during field deployment. Results demonstrate that this custom sUAS and sensor combination can detect radio tags at 100m above ground level and at horizontal ranges of 100m and 300m, with operators in either onshore or offshore locations. With this combination of sUAS and radio receiver, fish can be tracked in areas previously inaccessible and during flooding, providing new insights into riverine fish movement and habitat utilization.

  2. Gamma signatures of the C-BORD Tagged Neutron Inspection System

    Directory of Open Access Journals (Sweden)

    Sardet A.

    2018-01-01

    Full Text Available In the frame of C-BORD project (H2020 program of the EU, a Rapidly relocatable Tagged Neutron Inspection System (RRTNIS is being developed to non-intrusively detect explosives, chemical threats, and other illicit goods in cargo containers. Material identification is performed through gamma spectroscopy, using twenty NaI detectors and four LaBr3 detectors, to determine the different elements composing the inspected item from their specific gamma signatures induced by fast neutrons. This is performed using an unfolding algorithm to decompose the energy spectrum of a suspect item, selected by X-ray radiography and on which the RRTNIS inspection is focused, on a database of pure element gamma signatures. This paper reports on simulated signatures for the NaI and LaBr3 detectors, constructed using the MCNP6 code. First experimental spectra of a few elements of interest are also presented.

  3. Optimal Detection Range of RFID Tag for RFID-based Positioning System Using the k-NN Algorithm

    Directory of Open Access Journals (Sweden)

    Joon Heo

    2009-06-01

    Full Text Available Positioning technology to track a moving object is an important and essential component of ubiquitous computing environments and applications. An RFID-based positioning system using the k-nearest neighbor (k-NN algorithm can determine the position of a moving reader from observed reference data. In this study, the optimal detection range of an RFID-based positioning system was determined on the principle that tag spacing can be derived from the detection range. It was assumed that reference tags without signal strength information are regularly distributed in 1-, 2- and 3-dimensional spaces. The optimal detection range was determined, through analytical and numerical approaches, to be 125% of the tag-spacing distance in 1-dimensional space. Through numerical approaches, the range was 134% in 2-dimensional space, 143% in 3-dimensional space.

  4. Tagging the European eel Anguilla anguilla (L.) with coded wire tags

    DEFF Research Database (Denmark)

    Thomassen, S.; Pedersen, Michael Ingemann; Holdensgaard, G.

    2000-01-01

    The coded wire tag (CWT) system was examined as a possible tool for tagging European eels (Anguilla anguilla). Two size groups of eels (3.8 and 10.2 g) were tagged with CWTs in the dorsal musculature, Tag loss 28 days after tagging was 3.1% for the small and 0.7% for the large groups of eels...

  5. Enhanced Codeset Passive Wireless SAW Sensor-Tags and System, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed project will develop a set of at least 100 passive wireless surface acoustic wave (SAW) RFID sensor-tags for near-simultaneous remote monitoring of...

  6. Flavour tagging techniques for CPV studies in the Bs system with ATLAS

    CERN Document Server

    Jakoubek, T; The ATLAS collaboration

    2014-01-01

    The sensitivity to $B_s$ meson mixing phenomena can be significantly improved by distinguishing the initial meson flavour. We present the flavor tagging techniques, as used for instance in the ATLAS measurement of the angular amplitudes contributing to $B_s \\rightarrow J/\\psi(\\mu^+\\mu^-)\\phi(K^+K^-)$ decays. The methods, their performance and calibration on self-tagged $B^+ \\rightarrow J/\\psi K^+$ data are shown.

  7. RFID sensor-tags feeding a context-aware rule-based healthcare monitoring system.

    Science.gov (United States)

    Catarinucci, Luca; Colella, Riccardo; Esposito, Alessandra; Tarricone, Luciano; Zappatore, Marco

    2012-12-01

    Along with the growing of the aging population and the necessity of efficient wellness systems, there is a mounting demand for new technological solutions able to support remote and proactive healthcare. An answer to this need could be provided by the joint use of the emerging Radio Frequency Identification (RFID) technologies and advanced software choices. This paper presents a proposal for a context-aware infrastructure for ubiquitous and pervasive monitoring of heterogeneous healthcare-related scenarios, fed by RFID-based wireless sensors nodes. The software framework is based on a general purpose architecture exploiting three key implementation choices: ontology representation, multi-agent paradigm and rule-based logic. From the hardware point of view, the sensing and gathering of context-data is demanded to a new Enhanced RFID Sensor-Tag. This new device, de facto, makes possible the easy integration between RFID and generic sensors, guaranteeing flexibility and preserving the benefits in terms of simplicity of use and low cost of UHF RFID technology. The system is very efficient and versatile and its customization to new scenarios requires a very reduced effort, substantially limited to the update/extension of the ontology codification. Its effectiveness is demonstrated by reporting both customization effort and performance results obtained from validation in two different healthcare monitoring contexts.

  8. Architecture and Protocol of a Semantic System Designed for Video Tagging with Sensor Data in Mobile Devices

    Directory of Open Access Journals (Sweden)

    Alvaro Suarez

    2012-02-01

    Full Text Available Current mobile phones come with several sensors and powerful video cameras. These video cameras can be used to capture good quality scenes, which can be complemented with the information gathered by the sensors also embedded in the phones. For example, the surroundings of a beach recorded by the camera of the mobile phone, jointly with the temperature of the site can let users know via the Internet if the weather is nice enough to swim. In this paper, we present a system that tags the video frames of the video recorded from mobile phones with the data collected by the embedded sensors. The tagged video is uploaded to a video server, which is placed on the Internet and is accessible by any user. The proposed system uses a semantic approach with the stored information in order to make easy and efficient video searches. Our experimental results show that it is possible to tag video frames in real time and send the tagged video to the server with very low packet delay variations. As far as we know there is not any other application developed as the one presented in this paper.

  9. Architecture and Protocol of a Semantic System Designed for Video Tagging with Sensor Data in Mobile Devices

    Science.gov (United States)

    Macias, Elsa; Lloret, Jaime; Suarez, Alvaro; Garcia, Miguel

    2012-01-01

    Current mobile phones come with several sensors and powerful video cameras. These video cameras can be used to capture good quality scenes, which can be complemented with the information gathered by the sensors also embedded in the phones. For example, the surroundings of a beach recorded by the camera of the mobile phone, jointly with the temperature of the site can let users know via the Internet if the weather is nice enough to swim. In this paper, we present a system that tags the video frames of the video recorded from mobile phones with the data collected by the embedded sensors. The tagged video is uploaded to a video server, which is placed on the Internet and is accessible by any user. The proposed system uses a semantic approach with the stored information in order to make easy and efficient video searches. Our experimental results show that it is possible to tag video frames in real time and send the tagged video to the server with very low packet delay variations. As far as we know there is not any other application developed as the one presented in this paper. PMID:22438753

  10. Architecture and protocol of a semantic system designed for video tagging with sensor data in mobile devices.

    Science.gov (United States)

    Macias, Elsa; Lloret, Jaime; Suarez, Alvaro; Garcia, Miguel

    2012-01-01

    Current mobile phones come with several sensors and powerful video cameras. These video cameras can be used to capture good quality scenes, which can be complemented with the information gathered by the sensors also embedded in the phones. For example, the surroundings of a beach recorded by the camera of the mobile phone, jointly with the temperature of the site can let users know via the Internet if the weather is nice enough to swim. In this paper, we present a system that tags the video frames of the video recorded from mobile phones with the data collected by the embedded sensors. The tagged video is uploaded to a video server, which is placed on the Internet and is accessible by any user. The proposed system uses a semantic approach with the stored information in order to make easy and efficient video searches. Our experimental results show that it is possible to tag video frames in real time and send the tagged video to the server with very low packet delay variations. As far as we know there is not any other application developed as the one presented in this paper.

  11. Tag-elese or The Language of Tags

    Directory of Open Access Journals (Sweden)

    Jan Simons

    2008-01-01

    Full Text Available The core "meme" of Web 2.0 from which almost all other memes radiated was: 'You control your own data' (O'Reilly, 2005, 3. Key instruments for this user control are tagging systems that allow users to freely assign keywords of their own choosing to Internet resources of their own making as well as to documents produced by others. Of course, freely chosen keywords tags do not necessarily follow prefixed taxonomies or classification systems. But going by the maxim that interaction creates similarity and similarity creates interaction, the idea - or hope - is, however, that the tagging practices of individual users will eventually converge into an emergent common vocabulary or folksonomy (Merholz, 2004; Shirky, 2005; Vander Wal, 2005b; Mika, 2007. It is far from clear, however, that free tagging systems will eventually yield controlled vocabularies, and there are many incentives for idiosyncratic, ambiguous, and inconsistent uses of tags. Left to themselves, free tagging systems seem to be too wild and too chaotic for any order to emerge. But are these free tagging systems really as "feral" as they seem to be, or do they only look uncontrolled because one has been looking for order in the wrong place? I have done a quick-and-dirty" analysis of Flickr's tag cloud. The concept was: if folksonomies encourage users to tap on their own vernacular, everyday natural language must somehow "guide" the tagging practices of users of tagging systems. Flickr's tag cloud has been choosen because it may teach us something about tagging systems and folksonomies, and not - or not primarily - because of what tags may tell us about pictures.

  12. DNA barcode-based molecular identification system for fish species.

    Science.gov (United States)

    Kim, Sungmin; Eo, Hae-Seok; Koo, Hyeyoung; Choi, Jun-Kil; Kim, Won

    2010-12-01

    In this study, we applied DNA barcoding to identify species using short DNA sequence analysis. We examined the utility of DNA barcoding by identifying 53 Korean freshwater fish species, 233 other freshwater fish species, and 1339 saltwater fish species. We successfully developed a web-based molecular identification system for fish (MISF) using a profile hidden Markov model. MISF facilitates efficient and reliable species identification, overcoming the limitations of conventional taxonomic approaches. MISF is freely accessible at http://bioinfosys.snu.ac.kr:8080/MISF/misf.jsp .

  13. Interest Aware Location-Based Recommender System Using Geo-Tagged Social Media

    Directory of Open Access Journals (Sweden)

    Basma AlBanna

    2016-12-01

    Full Text Available Advances in location acquisition and mobile technologies led to the addition of the location dimension to Social Networks (SNs and to the emergence of a newer class called Location-Based Social Networks (LBSNs. While LBSNs are richer in their model and functions than SNs, they fail so far to attract as many users as SNs. On the other hand, SNs have large amounts of geo-tagged media that are under-utilized. In this paper, we propose an Interest-Aware Location-Based Recommender system (IALBR, which combines the advantages of both LBSNs and SNs, in order to provide interest-aware location-based recommendations. This recommender system is proposed as an extension to LBSNs. It is novel in: (1 utilizing the geo-content in both LBSNs and SNs; (2 ranking the recommendations based on a novel scoring method that maps to the user interests. It also works for passive users who are not active content contributors to the LBSN. This feature is critical to increase the number of LBSN users. Moreover, it helps with reducing the cold start problem, which is a common problem facing the new users of recommender systems who get random unsatisfying recommendations. This is due to the lack of user interest awareness, which is reliant on user history in most of the recommenders. We evaluated our system with a large-scale real dataset collected from foursquare with respect to precision, recall and the f-measure. We also compared the results with a ground truth system using metrics like the normalized discounted cumulative gain and the mean absolute error. The results confirm that the proposed IALBR generates more efficient recommendations than baselines in terms of interest awareness.

  14. Fresh fish ( Clarias gariepinus ) marketing system in major towns of ...

    African Journals Online (AJOL)

    This study was carried out to investigate marketing system, market channel, nature of costs, market margins and the factors determining the marketing profit of fresh fish (Clarias gariepinus) in major towns in Ibarapa Zone, Oyo state. Data were collected through the use of questionnaire administered to the fish traders.

  15. Internally readable identifying tag

    International Nuclear Information System (INIS)

    Jefferts, K.B.; Jefferts, E.R.

    1980-01-01

    A method of identifying non-metallic objects by means of X-ray equipment is described in detail. A small metal pin with a number of grooves cut in a pre-determined equi-spaced pattern is implanted into the non-metallic object and by decoding the groove patterns using X-ray equipment, the object is uniquely identified. A specific example of such an application is in studying the migratory habits of fish. The pin inserted into the snout of the fish is 0.010 inch in diameter, 0.040 inch in length with 8 possible positions for grooves if spaced 0.005 inch apart. With 6 of the groove positions available for data, the capacity is 2 6 or 64 combinations; clearly longer pins would increase the data capacity. This method of identification is a major advance over previous techniques which necessitated destruction of the fish in order to recover the identification tag. (UK)

  16. A Mobile Picture Tagging System Using Tree-Structured Layered Bayesian Networks

    Directory of Open Access Journals (Sweden)

    Young-Seol Lee

    2013-01-01

    Full Text Available Advances in digital media technology have increased in multimedia content. Tagging is one of the most effective methods to manage a great volume of multimedia content. However, manual tagging has limitations such as human fatigue and subjective and ambiguous keywords. In this paper, we present an automatic tagging method to generate semantic annotation on a mobile phone. In order to overcome the constraints of the mobile environment, the method uses two layered Bayesian networks. In contrast to existing techniques, this approach attempts to design probabilistic models with fixed tree structures and intermediate nodes. To evaluate the performance of this method, an experiment is conducted with data collected over a month. The result shows the efficiency and effectiveness of our proposed method. Furthermore, a simple graphic user interface is developed to visualize and evaluate recognized activities and probabilities.

  17. An SSVEP-actuated brain computer interface using phase-tagged flickering sequences: a cursor system.

    Science.gov (United States)

    Lee, Po-Lei; Sie, Jyun-Jie; Liu, Yu-Ju; Wu, Chi-Hsun; Lee, Ming-Huan; Shu, Chih-Hung; Li, Po-Hung; Sun, Chia-Wei; Shyu, Kuo-Kai

    2010-07-01

    This study presents a new steady-state visual evoked potential (SSVEP)-based brain computer interface (BCI). SSVEPs, induced by phase-tagged flashes in eight light emitting diodes (LEDs), were used to control four cursor movements (up, right, down, and left) and four button functions (on, off, right-, and left-clicks) on a screen menu. EEG signals were measured by one EEG electrode placed at Oz position, referring to the international EEG 10-20 system. Since SSVEPs are time-locked and phase-locked to the onsets of SSVEP flashes, EEG signals were bandpass-filtered and segmented into epochs, and then averaged across a number of epochs to sharpen the recorded SSVEPs. Phase lags between the measured SSVEPs and a reference SSVEP were measured, and targets were recognized based on these phase lags. The current design used eight LEDs to flicker at 31.25 Hz with 45 degrees phase margin between any two adjacent SSVEP flickers. The SSVEP responses were filtered within 29.25-33.25 Hz and then averaged over 60 epochs. Owing to the utilization of high-frequency flickers, the induced SSVEPs were away from low-frequency noises, 60 Hz electricity noise, and eye movement artifacts. As a consequence, we achieved a simple architecture that did not require eye movement monitoring or other artifact detection and removal. The high-frequency design also achieved a flicker fusion effect for better visualization. Seven subjects were recruited in this study to sequentially input a command sequence, consisting of a sequence of eight cursor functions, repeated three times. The accuracy and information transfer rate (mean +/- SD) over the seven subjects were 93.14 +/- 5.73% and 28.29 +/- 12.19 bits/min, respectively. The proposed system can provide a reliable channel for severely disabled patients to communicate with external environments.

  18. Surface Immobilized His-tagged Azurin as a Model Interface for the Investigation of Vectorial Electron Transfer in Biological Systems

    International Nuclear Information System (INIS)

    Casalini, Stefano; Berto, Marcello; Kovtun, Alessandro; Operamolla, Alessandra; Di Rocco, Giulia; Facci, Paolo; Liscio, Andrea; Farinola, Gianluca M.; Borsari, Marco; Bortolotti, Carlo A.

    2015-01-01

    A model system for the electrochemical investigation of vectorial electron transfer in biological systems was designed, assembled and characterized. Gold electrodes, functionalized with a -OCH_3 terminated, aromatic self-assembled monolayer, were used as a substrate for the adsorption of variants of copper-containing, redox metalloprotein azurin. The engineered azurin bears a polyhistidine tag at its C-terminus. Thanks to the presence of the solvent exposed tag, which chelates Cu"2"+ ions in solution, we introduced an exogenous redox centre. The different reduction potentials of the two redox centres and their positioning with respect to the surface are such that electron transfer from the exogenous copper centre and the electrode is mediated by the native azurin active site, closely paralleling electron transfer processes in naturally occurring multicentre metalloproteins.

  19. Fresh fish (Clarias gariepinus) marketing system in major towns of ...

    African Journals Online (AJOL)

    IJAAAR

    The study showed that women (85%) formed the highest number of fish traders in Ibarapa zone, women of age ... Marketing system is one of the main .... study area that marketing is a business activity ... important factor in the performance and.

  20. Fish abundance in the Wilderness and Swartvlei lake systems ...

    African Journals Online (AJOL)

    1995-11-06

    Nov 6, 1995 ... having a detrimental impact on fish communities, and propo- sals have been ... salinity gradient exists in the Wilderness system, with ...... Osmoregulation in juvenile Rhabdosargus .... Community metabolism and phosphorous.

  1. RESEARCH OF REFRIGERATION SYSTEMS FAILURES IN POLISH FISHING VESSELS

    Directory of Open Access Journals (Sweden)

    Waldemar KOSTRZEWA

    2013-07-01

    Full Text Available Temperature is a basic climatic parameter deciding about the quality change of fishing products. Time, after which qualitative changes of caught fish don’t exceed established, acceptable range, is above all the temperature function. Temperature reduction by refrigeration system of the cargo hold is a basic technical method, which allows extend transport time. Failures of refrigeration systems in fishing vessels have a negative impact on the environment in relation to harmful refrigerants emission. The paper presents the statistical analysis of failures occurred in the refrigeration systems of Polish fishing vessels in 2007‐2011 years. Analysis results described in the paper can be a base to draw up guidelines, both for designers as well as operators of the marine refrigeration systems.

  2. One of the three multiwire proportional chambers used in the photon tagging system at the Omega spectrometer

    CERN Multimedia

    CERN PhotoLab

    1976-01-01

    The momentum of incoming electrons, generated by SPS beams, is determined by magnets before they are directed onto a foil. The tagging system, a magnet and the MWPCs then determines the electron momentum after the foil and the difference between the two measurements gives the momentum of the photon which is heading for the spectrometer. The MWPCs were built in Daresburyand coupled with new CERN read0ut electronics.

  3. 77 FR 51761 - Proposed Information Collection; Comment Request; Groundfish Tagging Program

    Science.gov (United States)

    2012-08-27

    ... required by the Paperwork Reduction Act of 1995. DATES: Written comments must be submitted on or before... are two general categories of tags. Simple plastic tags (spaghetti tags) are external tags... fish. Archival tags are microchips with sensors encased in plastic cylinders that record the depth...

  4. Social Tagging of Mission Data

    Science.gov (United States)

    Norris, Jeffrey S.; Wallick, Michael N.; Joswig, Joseph C.; Powell, Mark W.; Torres, Recaredo J.; Mittman, David S.; Abramyan, Lucy; Crockett, Thomas M.; Shams, Khawaja S.; Fox, Jason M.; hide

    2010-01-01

    Mars missions will generate a large amount of data in various forms, such as daily plans, images, and scientific information. Often, there is a semantic linkage between images that cannot be captured automatically. Software is needed that will provide a method for creating arbitrary tags for this mission data so that items with a similar tag can be related to each other. The tags should be visible and searchable for all users. A new routine was written to offer a new and more flexible search option over previous applications. This software allows users of the MSLICE program to apply any number of arbitrary tags to a piece of mission data through a MSLICE search interface. The application of tags creates relationships between data that did not previously exist. These tags can be easily removed and changed, and contain enough flexibility to be specifically configured for any mission. This gives users the ability to quickly recall or draw attention to particular pieces of mission data, for example: Give a semantic and meaningful description to mission data; for example, tag all images with a rock in them with the tag "rock." Rapidly recall specific and useful pieces of data; for example, tag a plan as"driving template." Call specific data to a user s attention; for example, tag a plan as "for:User." This software is part of the MSLICE release, which was written in Java. It will run on any current Windows, Macintosh, or Linux system.

  5. Surgically Implanted JSATS Micro-Acoustic Transmitters Effects on Juvenile Chinook Salmon and Steelhead Tag Expulsion and Survival, 2010

    Energy Technology Data Exchange (ETDEWEB)

    Woodley, Christa M.; Carpenter, Scott M.; Carter, Kathleen M.; Wagner, Katie A.; Royer, Ida M.; Knox, Kasey M.; Kim, Jin A.; Gay, Marybeth E.; Weiland, Mark A.; Brown, Richard S.

    2011-09-16

    The purpose of this study was to evaluate survival model assumptions associated with a concurrent study - Acoustic Telemetry Evaluation of Dam Passage Survival and Associated Metrics at John Day, The Dalles, and Bonneville Dams, 2010 by Thomas Carlson and others in 2010 - in which the Juvenile Salmonid Acoustic Telemetry System (JSATS) was used to estimate the survival of yearling and subyearling Chinook salmon (Oncorhynchus tshawytscha) and steelhead (O. mykiss) migrating through the Federal Columbia River Power System (FCRPS). The micro-acoustic transmitter used in these studies is the smallest acoustic transmitter model to date (12 mm long x 5 mm wide x 4 mm high, and weighing 0.43 g in air). This study and the 2010 study by Carlson and others were conducted by researchers from the Pacific Northwest National Laboratory and the University of Washington for the U.S. Army Corps of Engineers, Portland District, to meet requirements set forth by the 2008 FCRPS Biological Opinion. In 2010, we compared survival, tag burden, and tag expulsion in five spring groups of yearling Chinook salmon (YCH) and steelhead (STH) and five summer groups of subyearling Chinook salmon (SYC) to evaluate survival model assumptions described in the concurrent study. Each tagging group consisted of approximately 120 fish/species, which were collected and implanted on a weekly basis, yielding approximately 600 fish total/species. YCH and STH were collected and implanted from late April to late May (5 weeks) and SYC were collected and implanted from mid-June to mid-July (5 weeks) at the John Day Dam Smolt Monitoring Facility. The fish were collected once a week, separated by species, and assigned to one of three treatment groups: (1) Control (no surgical treatment), (2) Sham (surgical implantation of only a passive integrated transponder [PIT] tag), and (3) Tagged (surgical implantation of JSATS micro-acoustic transmitter [AT] and PIT tags). The test fish were held for 30 days in indoor

  6. Innovative water withdrawal system re-establishes fish migration runs

    International Nuclear Information System (INIS)

    Anon.

    2008-01-01

    This article described a unique water withdrawal and fish bypass structure that is under construction in Oregon to re-establish anadromous fish runs and to improve water quality downstream of the Round Butte dam. Portland General Electric and the Confederated Tribes of the Warm Springs Reservation, which co-own the dam, have committed to re-establish fish runs in response to concerns over the declining numbers of salmon and trout in the region. Water intakes are routinely added at hydroelectric facilities to protect native fish in compliance with the Federal Energy Regulatory Commission and the Clean Water Act. The Round Butte Hydroelectric project had a complex set of challenges whereby surface-current directions had to be changed to help migrating salmon swim easily into a fish handling area and create a fish collection system. CH2M HILL designed the system which consists of a large floating structure, an access bridge, a large vertical conduit and a base structure resting on the lake bed. Instead of using 2D CAD file methods, CH2M HILL decided to take advantage of 3D models to visualize the complex geometry of these structures. The 3D models were used to help designers and consultants understand the issues, resolve conflicts and design solutions. The objective is to have the system operating by the 2009 migrating season. 1 ref., 4 figs

  7. Recommender systems and the social web leveraging tagging data for recommender systems

    CERN Document Server

    Gedikli, Fatih

    2013-01-01

    There is an increasing demand for recommender systems due to the information overload users are facing on the Web. The goal of a recommender system is to provide personalized recommendations of products or services to users. With the advent of the Social Web, user-generated content has enriched the social dimension of the Web. As user-provided content data also tells us something about the user, one can learn the user's individual preferences from the Social Web. This opens up completely new opportunities and challenges for recommender systems research. Fatih Gedikli deals with the question of

  8. Design and Analysis of Salmonid Tagging Studies in the Columbia Basin, Volume XIII; Appraisal of System-Wide Survival Estimation of Snake River Yearling Chinook Salmon Released in 1997 and 1988, Using PIT-Tags Recovered from Caspian Tern and Double-Crested Cormorant Breeding Colonies on Rice Island, 1997-1998 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Skalski, John R.; Perez-Comas, Jose A. (University of Washington, School of Fisheries, Seattle, WA)

    2000-05-01

    PIT-tags recovered from tern and cormorant breeding colonies at Rice Island and observations from the interrogation systems at John Day and Bonneville Dams were incorporated into survival analyses. Whether the estimates for the upper reaches of the system, between Lower Granite and McNary Dams were as expected (with weighted averages S{sub LGR-LGS} = 0.996, S{sub LGS-LMN} = 0.837, and S{sub LMN-McN} = 0.941), those for the lower reaches, between John Day and Bonneville Dams, appeared positively biased with survival estimates typically greater than 1. Their weighted averages were S{sub McN-JDA} = 0.707 and S{sub JDA-BON} = 1.792 for 1997 releases. For the 1998 releases, they were S{sub McN-JDA} = 0.795 and S{sub JDA-BON} = 1.312. If the estimates for the lower reaches were biased, the estimates for the whole project would also be biased (S{sub LGR-BON} = 0.819). We determined that bias could have arisen if the terns and cormorants of Rice Island fished for salmon yearlings in waters of the BON-Rice reach at low rates (M{sub BON-Rice} {le} 0.2), and the rates of tag-deposition and tag-detection were low (R{sub D} x R{sub R} {le} 0.4). Moreover, unknown levels of uncensored post-detection mortality and scavenging of previously dead salmon yearlings may have also added to the bias.

  9. A new tagging system for production of recombinant proteins in Drosophila S2 cells using the third domain of the urokinase receptor

    DEFF Research Database (Denmark)

    Gårdsvoll, Henrik; Hansen, Line V; Jørgensen, Thomas J D

    2007-01-01

    The use of protein fusion tag technology greatly facilitates detection, expression and purification of recombinant proteins, and the demands for new and more effective systems are therefore expanding. We have used a soluble truncated form of the third domain of the urokinase receptor...... as a convenient C-terminal fusion partner for various recombinant extracellular human proteins used in basic cancer research. The stability of this cystein-rich domain, which structure adopts a three-finger fold, provides an important asset for its applicability as a fusion tag for expression of recombinant...... chromatography procedure using the immobilized anti-uPAR monoclonal antibody R2. An optional enterokinase cleavage site is included between the various recombinant proteins and the linker region of the tag, which enables generation of highly pure preparations of tag-free recombinant proteins. Using this system...

  10. A Bioassay System Using Bioelectric Signals from Small Fish

    Science.gov (United States)

    Terawaki, Mitsuru; Soh, Zu; Hirano, Akira; Tsuji, Toshio

    Although the quality of tap water is generally examined using chemical assay, this method cannot be used for examination in real time. Against such a background, the technique of fish bioassay has attracted attention as an approach that enables constant monitoring of aquatic contamination. The respiratory rhythms of fish are considered an efficient indicator for the ongoing assessment of water quality, since they are sensitive to chemicals and can be indirectly measured from bioelectric signals generated by breathing. In order to judge aquatic contamination accurately, it is necessary to measure bioelectric signals from fish swimming freely as well as to stably discriminate measured signals, which vary between individuals. However, no bioassay system meeting the above requirements has yet been established. This paper proposes a bioassay system using bioelectric signals generated from small fish in free-swimming conditions. The system records signals using multiple electrodes to cover the extensive measurement range required in a free-swimming environment, and automatically discriminates changes in water quality from signal frequency components. This discrimination is achieved through an ensemble classification method using probability neural networks to solve the problem of differences between individual fish. The paper also reports on the results of related validation experiments, which showed that the proposed system was able to stably discriminate between water conditions before and after bleach exposure.

  11. TagPad

    DEFF Research Database (Denmark)

    Bornoe, Nis; Barkhuus, Louise

    2013-01-01

    The area of cyberinfrastructures has looked extensively at research within the natural sciences, however, the social sciences have been largely overlooked in terms of novel data collection and analysis systems. We developed a probe tool, TagPad, to look at the process for social science data...

  12. Automatic system for monitoring fish passage at dams

    Science.gov (United States)

    Castignolles, Nathalie; Cattoen, Michel; Larinier, M.

    1994-09-01

    Devices called fishways or fish passes are constructed in rivers to help migratory fish get over obstacles (dams). There counting windows are used to monitor fish passage by video-based counting. Our goal is to design and construct a vision system to automate this process. Images are taken by a video camera fitted with an electronic shutter in a backlit fishway. They are stored on optical disks in real time but are processed in delayed time. Faced with high volumes of data, a compression is necessary and an electronic board has been designed to accomplish it in real time. The coding method used is based on a run description of binarized images. Then, a tracking process is implemented on a micro-computer to count the fish crossing the pass. It includes fish recognition, which is based on a Bayesian classification process. In order to reduce processing times, recognition operations (labelling, parameter extraction) are accomplished on coded images. Classification results are satisfactory and are improved by the temporal redundancy generated by the tracking process. Image processing time permits the user, on average, to process images faster than they have been stored. Thus there is no data accumulation. At the end of the processing it is possible to edit a result file, to choose a fish, view its crossing images and change its species if wrong.

  13. An Automated System for Incubation of Pelagic Fish Eggs

    Directory of Open Access Journals (Sweden)

    Leif Jørgensen

    1987-01-01

    Full Text Available An automated system for incubation of pelagic fish eggs is described. The system has an internal air driven water circulation which separates healthy and dead or strongly infected eggs. A processor controlled, pulsed water exchange provides a strongly reduced water requirement. The equipment has also an automated temperature and salinity control and adjustment.

  14. Fish abundance in the Wilderness and Swartvlei lake systems ...

    African Journals Online (AJOL)

    A longer duration tidal phase in the Swartvlei system during 1992 and 1993, compared to the Wilderness lake system, did not result in greater abundance of fish sampled. There appears to be no justification for the artificial maintenance of permanently tidal conditions in the Swartvlei and Touw River estuaries on the ...

  15. Nonnative Fishes in the Upper Mississippi River System

    Science.gov (United States)

    Irons, Kevin S.; DeLain, Steven A.; Gittinger, Eric; Ickes, Brian S.; Kolar, Cindy S.; Ostendort, David; Ratcliff, Eric N.; Benson, Amy J.; Irons, Kevin S.

    2009-01-01

    The introduction, spread, and establishment of nonnative species is widely regarded as a leading threat to aquatic biodiversity and consequently is ranked among the most serious environmental problems facing the United States today. This report presents information on nonnative fish species observed by the Long Term Resource Monitoring Program on the Upper Mississippi River System a nexus of North American freshwater fish diversity for the Nation. The Long Term Resource Monitoring Program, as part of the U.S. Army Corps of Engineers' Environmental Management Plan, is the Nation's largest river monitoring program and stands as the primary source of standardized ecological information on the Upper Mississippi River System. The Long Term Resource Monitoring Program has been monitoring fish communities in six study areas on the Upper Mississippi River System since 1989. During this period, more than 3.5 million individual fish, consisting of 139 species, have been collected. Although fish monitoring activities of the Long Term Resource Monitoring Program focus principally on entire fish communities, data collected by the Program are useful for detecting and monitoring the establishment and spread of nonnative fish species within the Upper Mississippi River System Basin. Sixteen taxa of nonnative fishes, or hybrids thereof, have been observed by the Long Term Resource Monitoring Program since 1989, and several species are presently expanding their distribution and increasing in abundance. For example, in one of the six study areas monitored by the Long Term Resource Monitoring Program, the number of established nonnative species has increased from two to eight species in less than 10 years. Furthermore, contributions of those eight species can account for up to 60 percent of the total annual catch and greater than 80 percent of the observed biomass. These observations are critical because the Upper Mississippi River System stands as a nationally significant pathway for

  16. [Functional development of chemosensory systems in the ontogeny of fish].

    Science.gov (United States)

    Kasumian, A O

    2011-01-01

    Regularities of the functional development of chemosensory systems in the ontogeny of fish has been studied, i.e., the olfactory system, the taste system, and the common chemical sense. The olfactory system begins to function and provides response of juveniles to chemical signals before the taste system. Embryos that have hatched from coating but that do not yet feed exhibit nonspecialized motor responses to olfactory stimuli already. Immediately after the transition to exogenous nutrition, olfactory sensitivity to signals which elicit defensive and feeding behavioral responses begins to form and the ability to differentiate between similar odors develops. The reception of a limited number of taste stimuli occurs in the larvae during the transition to exogenous nutrition. With age, the spectrum of effective taste substances expands and the time spent on the definition of palatability by juvenile fishes reduces. Functional development of individual components of the taste system arises heterochronously, i.e., the outer (extraoral) form of taste reception arises earlier and more rapidly, and the buccal (intraoral) form of taste reception arises slower. No information is available about the functional development of the common chemical sense in the ontogeny of fish. It is assumed that the function of the chemosensory system arises in fish in early larval instar.

  17. Lipophilized phenolics as antioxidants in fish oil enriched food systems

    DEFF Research Database (Denmark)

    Sørensen, Ann-Dorit Moltke; Nielsen, Nina Skall; Jacobsen, Charlotte

    Food products containing long chain omega-3 PUFA are highly susceptible to oxidation, which causes undesirable flavors and loss of health beneficial fatty acids. Many omega-3 enriched food products on the market are oil-in-water emulsions. According to the so called “polar paradox”, polar compounds...... hypothesis is that lipophilization of such polar phenolic compounds may improve their efficacy in fish oil enriched food systems. Our study aimed at evaluating rutin and dihydrocaffeic acid and their esters as antioxidants in o/w emulsion model system and milk enriched with fish oil. Moreover, the effect...

  18. Using Gunshot Detection Systems to Fight Explosive Fishing Practices

    Science.gov (United States)

    Showen, R. L.; Dunson, J. C.; Woodman, G.; Christopher, S.; Wilson, S.

    2015-12-01

    Blast fishing (using explosives to catch fish) causes extensive damage to coral reefs, especially in the Coral Triangle in Southeast Asia. Subsistence fishermen and larger consortiums, often with criminal links, throw an explosive into a school of fish, killing all sea life within range. This unsustainable practice is becoming more prevalent, and threatens the protein supply of as many as a billion people. Ending blast fishing will require combined technical and societal methods aimed at both deterring the practice, and catching those responsible. Our work aims to significantly improve enforcement. We are re-purposing SST's ShotSpotter gunshot detection system, (trusted and valued by police around the world), substituting hydrophones for the present microphones. Using multilateration and trained human reviewers, the system can give prompt blast alerts, location data, and acoustic waveforms to law enforcement officials. We hope to establish a prototype system in Malaysia in 2015, and have already secured governmental approvals for installation and tests with local law enforcement. The Scubazoo media firm in Malaysia is working with resorts, dive operations, and celebrity sponsors, and is planning to produce videos to illustrate the severity of the problem to both governments and the public. Because there is little hard data concerning the prevalence of blast fishing in either marine protected areas or open waters, the system can also indicate to the world the actual blast rates and patterns of use. The Teng Hoi environmental NGO in Hong Kong showed in 2004 that acoustic waves from typical bombs propagate on the order of 20 km, so an underwater locator system with a small number of sensors can feasibly cover a sizable coral region. Our present plans are to mount sensors on piers, buoys, and boats, but if possible we would also like to integrate with other existing acoustic arrays to strengthen the fight against blast fishing.

  19. An efficient tag derived from the common epitope of tospoviral NSs proteins for monitoring recombinant proteins expressed in both bacterial and plant systems.

    Science.gov (United States)

    Cheng, Hao-Wen; Chen, Kuan-Chun; Raja, Joseph A J; Li, Jian-Xian; Yeh, Shyi-Dong

    2013-04-15

    NSscon (23 aa), a common epitope in the gene silencing suppressor NSs proteins of the members of the Watermelon silver mottle virus (WSMoV) serogroup, was previously identified. In this investigation, we expressed different green fluorescent protein (GFP)-fused deletions of NSscon in bacteria and reacted with NSscon monoclonal antibody (MAb). Our results indicated that the core 9 amino acids, "(109)KFTMHNQIF(117)", denoted as "nss", retain the reactivity of NSscon. In bacterial pET system, four different recombinant proteins labeled with nss, either at N- or C-extremes, were readily detectable without position effects, with sensitivity superior to that for the polyhistidine-tag. When the nss-tagged Zucchini yellow mosaic virus (ZYMV) helper component-protease (HC-Pro) and WSMoV nucleocapsid protein were transiently expressed by agroinfiltration in tobacco, they were readily detectable and the tag's possible efficacy for gene silencing suppression was not noticed. Co-immunoprecipitation of nss-tagged and non-tagged proteins expressed from bacteria confirmed the interaction of potyviral HC-Pro and coat protein. Thus, we conclude that this novel nss sequence is highly valuable for tagging recombinant proteins in both bacterial and plant expression systems. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. An Design of HF-Band RFID System with Multiple Readers and Passive Tags for Indoor Mobile Robot Self-Localization

    Directory of Open Access Journals (Sweden)

    Jian Mi

    2016-07-01

    Full Text Available Radio frequency identification (RFID technology has already been explored for efficient self-localization of indoor mobile robots. A mobile robot equipped with RFID readers detects passive RFID tags installed on the floor in order to locate itself. The Monte-Carlo localization (MCL method enables the localization of a mobile robot equipped with an RFID system with reasonable accuracy, sufficient robustness and low computational cost. The arrangements of RFID readers and tags and the size of antennas are important design parameters for realizing accurate and robust self-localization using a low-cost RFID system. The design of a likelihood model of RFID tag detection is also crucial for the accurate self-localization. This paper presents a novel design and arrangement of RFID readers and tags for indoor mobile robot self-localization. First, by considering small-sized and large-sized antennas of an RFID reader, we show how the design of the likelihood model affects the accuracy of self-localization. We also design a novel likelihood model by taking into consideration the characteristics of the communication range of an RFID system with a large antenna. Second, we propose a novel arrangement of RFID tags with eight RFID readers, which results in the RFID system configuration requiring much fewer readers and tags while retaining reasonable accuracy of self-localization. We verify the performances of MCL-based self-localization realized using the high-frequency (HF-band RFID system with eight RFID readers and a lower density of RFID tags installed on the floor based on MCL in simulated and real environments. The results of simulations and real environment experiments demonstrate that our proposed low-cost HF-band RFID system realizes accurate and robust self-localization of an indoor mobile robot.

  1. Columbia River System Operation Review : Final Environmental Impact Statement, Appendix C: Anadromous Fish and Juvenile Fish Transportation.

    Energy Technology Data Exchange (ETDEWEB)

    Columbia River System Operation Review (U.S.)

    1995-11-01

    This Appendix C of the Final Environmental Impact Statement for the Columbia River System discusses impacts on andromous fish and juvenile fish transportation. The principal andromous fish in the Columbia basin include salmonid species (Chinook, coho, and sockeye salmon, and steelhead) and nonsalmoinid andromous species (sturgeon, lamprey, and shad). Major sections in this document include the following: background, scope and process; affected environment for salmon and steelhead, shaded, lamprey, sturgeon; study methods; description of alternatives: qualitative and quantitative findings.

  2. An integrated fish-plankton aquaculture system in brackish water.

    Science.gov (United States)

    Gilles, S; Fargier, L; Lazzaro, X; Baras, E; De Wilde, N; Drakidès, C; Amiel, C; Rispal, B; Blancheton, J-P

    2013-02-01

    Integrated Multi-Trophic Aquaculture takes advantage of the mutualism between some detritivorous fish and phytoplankton. The fish recycle nutrients by consuming live (and dead) algae and provide the inorganic carbon to fuel the growth of live algae. In the meanwhile, algae purify the water and generate the oxygen required by fishes. Such mechanism stabilizes the functioning of an artificially recycling ecosystem, as exemplified by combining the euryhaline tilapia Sarotherodon melanotheron heudelotii and the unicellular alga Chlorella sp. Feed addition in this ecosystem results in faster fish growth but also in an increase in phytoplankton biomass, which must be limited. In the prototype described here, the algal population control is exerted by herbivorous zooplankton growing in a separate pond connected in parallel to the fish-algae ecosystem. The zooplankton production is then consumed by tilapia, particularly by the fry and juveniles, when water is returned to the main circuit. Chlorella sp. and Brachionus plicatilis are two planktonic species that have spontaneously colonized the brackish water of the prototype, which was set-up in Senegal along the Atlantic Ocean shoreline. In our system, water was entirely recycled and only evaporation was compensated (1.5% volume/day). Sediment, which accumulated in the zooplankton pond, was the only trophic cul-de-sac. The system was temporarily destabilized following an accidental rotifer invasion in the main circuit. This caused Chlorella disappearance and replacement by opportunist algae, not consumed by Brachionus. Following the entire consumption of the Brachionus population by tilapias, Chlorella predominated again. Our artificial ecosystem combining S. m. heudelotii, Chlorella and B. plicatilis thus appeared to be resilient. This farming system was operated over one year with a fish productivity of 1.85 kg/m2 per year during the cold season (January to April).

  3. The immune system of cyprinid fish = Immunologie van de karper

    NARCIS (Netherlands)

    Rijkers, G.T.

    1980-01-01

    This study deals with several aspects of the immune system of cyprinid fish.

    Some observations on the development of cellular and humoral responsiveness in rosy barb (Barbus conchonius) are described in appendix I. A humoral anti-sheep red blood cell (SRBC) response was demonstrated in

  4. Yellowtail Tagging Data (MRDBS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Yellowtail Flounder Tagging Program began in 2003 and works with commercial fishermen to tag and release yellowtaiI flounder with pink and yellow disc tags or...

  5. Stressing fish in Recirculating Aquaculture Systems (RAS): Does stress induced in one group of fish affect the feeding motivation of other fish sharing the same RAS?

    NARCIS (Netherlands)

    Martins, C.I.; Eding, E.H.; Verreth, J.A.J.

    2011-01-01

    As a consequence of water re-use and high stocking densities, Recirculating Aquaculture Systems (RAS) may lead to an accumulation of substances released by the fish into the water, e.g. cortisol and alarm pheromones. This study investigated the effect of stressing fish on the feeding motivation of

  6. THE INFORMATIONAL SYSTEM FOR RESOURSES ADMINISTRATION IN FISH FARMS

    Directory of Open Access Journals (Sweden)

    Adrian ZUGRAVU

    2005-01-01

    Full Text Available The informational system for aquaculture activities provide a financial planning and analysis tool.The software can also be of assistance to land-based farmers who want to more thoroughly utilizetheir water resources by developing small-scale fish farm systems to provide supplementary income.Informational model has been enhanced to produce a comprehensive software package foraquaculture feasibility modeling, financial planning, sales and harvesting planning and managementinformation tools.

  7. Understanding why users tag: A survey of tagging motivation literature and results from an empirical study.

    Science.gov (United States)

    Strohmaier, Markus; Körner, Christian; Kern, Roman

    2012-12-01

    While recent progress has been achieved in understanding the structure and dynamics of social tagging systems, we know little about the underlying user motivations for tagging, and how they influence resulting folksonomies and tags. This paper addresses three issues related to this question. (1) What distinctions of user motivations are identified by previous research, and in what ways are the motivations of users amenable to quantitative analysis? (2) To what extent does tagging motivation vary across different social tagging systems? (3) How does variability in user motivation influence resulting tags and folksonomies? In this paper, we present measures to detect whether a tagger is primarily motivated by categorizing or describing resources, and apply these measures to datasets from seven different tagging systems. Our results show that (a) users' motivation for tagging varies not only across, but also within tagging systems, and that (b) tag agreement among users who are motivated by categorizing resources is significantly lower than among users who are motivated by describing resources . Our findings are relevant for (1) the development of tag-based user interfaces, (2) the analysis of tag semantics and (3) the design of search algorithms for social tagging systems.

  8. Comparison of three nonlinear models to describe long-term tag shedding by lake trout

    Science.gov (United States)

    Fabrizio, Mary C.; Swanson, Bruce L.; Schram, Stephen T.; Hoff, Michael H.

    1996-01-01

    We estimated long-term tag-shedding rates for lake trout Salvelinus namaycush using two existing models and a model we developed to account for the observed permanence of some tags. Because tag design changed over the course of the study, we examined tag-shedding rates for three types of numbered anchor tags (Floy tags FD-67, FD-67C, and FD-68BC) and an unprinted anchor tag (FD-67F). Lake trout from the Gull Island Shoal region, Lake Superior, were double-tagged, and subsequent recaptures were monitored in annual surveys conducted from 1974 to 1992. We modeled tag-shedding rates, using time at liberty and probabilities of tag shedding estimated from fish released in 1974 and 1978–1983 and later recaptured. Long-term shedding of numbered anchor tags in lake trout was best described by a nonlinear model with two parameters: an instantaneous tag-shedding rate and a constant representing the proportion of tags that were never shed. Although our estimates of annual shedding rates varied with tag type (0.300 for FD-67, 0.441 for FD-67C, and 0.656 for FD-68BC), differences were not significant. About 36% of tags remained permanently affixed to the fish. Of the numbered tags that were shed (about 64%), two mechanisms contributed to tag loss: disintegration and dislodgment. Tags from about 11% of recaptured fish had disintegrated, but most tags were dislodged. Unprinted tags were shed at a significant but low rate immediately after release, but the long-term, annual shedding rate of these tags was only 0.013. Compared with unprinted tags, numbered tags dislodged at higher annual rates; we hypothesized that this was due to the greater frictional drag associated with the larger cross-sectional area of numbered tags.

  9. Technologies for the marking of fishing gear to identify gear components entangled on marine animals and to reduce abandoned, lost or otherwise discarded fishing gear.

    Science.gov (United States)

    He, Pingguo; Suuronen, Petri

    2018-04-01

    Fishing gears are marked to establish and inform origin, ownership and position. More recently, fishing gears are marked to aid in capacity control, reduce marine litter due to abandoned, lost or otherwise discarded fishing gear (ALDFG) and assist in its recovery, and to combat illegal, unreported and unregulated (IUU) fishing. Traditionally, physical marking, inscription, writing, color, shape, and tags have been used for ownership and capacity purposes. Buoys, lights, flags, and radar reflectors are used for marking of position. More recently, electronic devices have been installed on marker buoys to enable easier relocation of the gear by owner vessels. This paper reviews gear marking technologies with focus on coded wire tags, radio frequency identification tags, Automatic Identification Systems, advanced electronic buoys for pelagic longlines and fish aggregating devices, and re-location technology if the gear becomes lost. Copyright © 2018 Elsevier Ltd. All rights reserved.

  10. Yolo Bypass Juvenile Salmon Utilization Study 2016—Summary of acoustically tagged juvenile salmon and study fish release, Sacramento River, California

    Science.gov (United States)

    Liedtke, Theresa L.; Hurst, William R.

    2017-09-12

    The Yolo Bypass is a flood control bypass in Sacramento Valley, California. Flood plain habitats may be used for juvenile salmon rearing, however, the potential value of such habitats can be difficult to evaluate because of the intermittent nature of inundation events. The Yolo Bypass Juvenile Salmon Utilization Study (YBUS) used acoustic telemetry to evaluate the movements and survival of juvenile salmon adjacent to and within the Yolo Bypass during the winter of 2016. This report presents numbers, size data, and release data (times, dates, and locations) for the 1,197 acoustically tagged juvenile salmon released for the YBUS from February 21 to March 18, 2016. Detailed descriptions of the surgical implantation of transmitters are also presented. These data are presented to support the collaborative, interagency analysis and reporting of the study findings.

  11. The role of the nervous system in fish evolution

    Directory of Open Access Journals (Sweden)

    Michael H Hofmann

    2015-12-01

    Full Text Available The nervous system plays an important role in the evolution and adaptation of animals. All sensory and motor functions as well as cognitive abilities are organized in the brain and spinal cord. Volumetric measurements of different brain regions were made in more than 150 species of ray finned fishes as well as in several outgroups. In Actanthopterygii, the hypothalamus shows greatest enlargement most likely due to an enormous visual input via the nucleus glomerulosos. The telencephalon is highly differentiated in many acanthopterygii, mostly coral reef species, but its relative size is not much effected. There is, however, a clear shift from olfactory to visual functions in ray finned fishes. In species with a highly differentiated telencephalon, the area where place memory may be located is very prominent. In basal ray finned fishes, lungfish, amphibia and elasmobranchs, the olfactory bulb is relatively large and the ratio of the olfactory bulb and telencephalon large as well. This holds also for elopomorpha and spiny eels, but in most other groups vision dominates. Apart from differences between larger clades, variation in brain architecture are also seen in closely related species and even between sexes of the same species. Profound differences are present in the cerebellum between male and female swordtails and in the telencephalon of sticklebacks. Morphometric analysis of brain architecture turned out to be an important tool to study the evolution and adaptations of the brain in fishes.

  12. Thyroid hormone actions on male reproductive system of teleost fish.

    Science.gov (United States)

    Tovo-Neto, Aldo; da Silva Rodrigues, Maira; Habibi, Hamid R; Nóbrega, Rafael Henrique

    2018-04-17

    Thyroid hormones (THs) play important roles in the regulation of many biological processes of vertebrates, such as growth, metabolism, morphogenesis and reproduction. An increasing number of studies have been focused on the involvement of THs in the male reproductive system of vertebrates, in particular of fish. Therefore, this mini-review aims to summarize the main findings on THs role in male reproductive system of fish, focusing on sex differentiation, testicular development and spermatogenesis. The existing data in the literature have demonstrated that THs exert their roles at the different levels of the hypothalamic-pituitary-gonadal (HPG) axis. In general a positive correlation has been shown between THs and fish reproductive status; where THs are associated with testicular development, growth and maturation. Recently, the molecular mechanisms underlying the role of THs in spermatogenesis have been unraveled in zebrafish testis. THs promote germ cell proliferation and differentiation by increasing a stimulatory growth factor of spermatogenesis produced by Sertoli cells. In addition, THs enhanced the gonadotropin-induced androgen release in zebrafish testis. Next to their functions in the adult testis, THs are involved in the gonadal sex differentiation through modulating sex-related gene expression, and testicular development via regulation of Sertoli cell proliferation. In conclusion, this mini-review showed that THs modulate the male reproductive system during the different life stages of fish. The physiological and molecular mechanisms showed a link between the thyroid and reproduction, suggesting a possibly co-evolution and interdependence of these two systems. Copyright © 2018 Elsevier Inc. All rights reserved.

  13. Fish ladders: safe fish passage or hotspot for predation?

    Directory of Open Access Journals (Sweden)

    Angelo Antonio Agostinho

    Full Text Available Fish ladders are a strategy for conserving biodiversity, as they can provide connectivity between fragmented habitats and reduce predation on shoals that accumulate immediately below dams. Although the impact of predation downstream of reservoirs has been investigated, especially in juvenile salmonids during their downstream movements, nothing is known about predation on Neotropical fish in the attraction and containment areas commonly found in translocation facilities. This study analysed predation in a fish passage system at the Lajeado Dam on the Tocantins River in Brazil. The abundance, distribution, and the permanence (time spent of large predatory fish along the ladder, the injuries imposed by piranhas during passage and the presence of other vertebrate predators were investigated. From December 2002 to October 2003, sampling was conducted in four regions (downstream, along the ladder, in the forebay, and upstream of the reservoir using gillnets, cast nets and counts or visual observations. The captured fish were tagged with thread and beads, and any mutilations were registered. Fish, birds and dolphins were the main predator groups observed, with a predominance of the first two groups. The entrance to the ladder, in the downstream region, was the area with the highest number of large predators and was the only region with relevant non-fish vertebrates. The main predatory fish species were Rhaphiodon vulpinus, Hydrolycus armatus, and Serrasalmus rhombeus. Tagged individuals were detected predating along the ladder for up to 90 days. Mutilations caused by Serrasalmus attacks were noted in 36% of species and 4% of individuals at the top of the ladder. Our results suggested that the high density of fish in the restricted ladder environment, which is associated with injuries suffered along the ladder course and the presence of multiple predator groups with different predation strategies, transformed the fish corridor into a hotspot for

  14. Secure passive RFID tag with seal

    Science.gov (United States)

    Nekoogar, Faranak; Reynolds, Matthew; Lefton, Scott; Dowla, Farid; Twogood, Richard

    2017-11-14

    A secure passive RFID tag system comprises at least one base station and at least one passive RFID tag. The tag includes a fiber optic cable with the cable ends sealed within the tag and the middle portion forming an external loop. The loop may be secured to at least portions of an object. The tag transmits and receives an optical signal through the fiber optic cable, and the cable is configured to be damaged or broken in response to removal or tampering attempts, wherein the optical signal is significantly altered if the cable is damaged or broken. The tag transmits the optical signal in response to receiving a radio signal from the base station and compares the transmitted optical signal to the received optical signal. If the transmitted optical signal and the received optical signal are identical, the tag transmits an affirmative radio signal to the base station.

  15. Impact of cooling systems on Lake Michigan fishes

    International Nuclear Information System (INIS)

    Spigarelli, S.A.; Romberg, G.P.

    1976-01-01

    A comparison of data on fish mortalities due to impingement at thermal power plant water intakes on Lake Michigan with available estimates of standing crop biomass, commercial and sport fishery catches, and estimated predation mortality is presented. The striking features of these data are the proportions of total mortality due to predation and the lack of accurate basic population statistics such as standing crop biomass and natural mortality for important forage and human food fishes in Lake Michigan. Although this preliminary assessment would indicate that power plant and total impingement losses constitute an insignificant fraction of total forage biomass, the potentially unstable forage-predator ratios and the apparent high degree of annual fluctuations (year-classes) in alewife, smelt, and perch indicate the need for a more detailed assessment of cooling-system related impact on selected populations

  16. Methow and Columbia Rivers studies: summary of data collection, comparison of database structure and habitat protocols, and impact of additional PIT tag interrogation systems to survival estimates, 2008-2012

    Science.gov (United States)

    Martens, Kyle D.; Tibbits, Wesley T.; Watson, Grace A.; Newsom, Michael A.; Connolly, Patrick J.

    2014-01-01

    designed to show some initial analysis and to disseminate summary information that could potentially be used in ongoing modeling efforts by USGS, Reclamation, and University of Idaho. The second chapter documents the database of fish and habitat data collected by USGS from 2004 through 2012 and compares USGS habitat protocols to the Columbia Habitat Monitoring Program (CHaMP) protocol. The third chapter is a survival analysis of fish moving through Passive Integrated Transponder (PIT) tag interrogation systems in the Methow and Columbia Rivers. It examines the effects of adding PIT tags and/or PIT tag interrogation systems on survival estimates of juvenile steelhead and Chinook salmon.

  17. Methodologies for Improved Tag Cloud Generation with Clustering

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2012-01-01

    Tag clouds are useful means for navigation in the social web systems. Usually the systems implement the tag cloud generation based on tag popularity which is not always the best method. In this paper we propose methodologies on how to combine clustering into the tag cloud generation to improve...... coverage and overlap. We study several clustering algorithms to generate tag clouds. We show that by extending cloud generation based on tag popularity with clustering we slightly improve coverage. We also show that if the cloud is generated by clustering independently of the tag popularity baseline we...

  18. Scanning for PIT-tagged flatfish in a coastal area using a sledge equipped with an RFID antenna

    DEFF Research Database (Denmark)

    Sparrevohn, Claus Reedtz; Aarestrup, Kim; Stenberg, Claus

    2014-01-01

    A radio frequency identification (RFID) antenna system, build into a sledge that can be towed behind a vessel like a trawl and thereby has the potential to detect the position of a passive inductor technology (PIT)-tagged fish in a wide variety of habitats, is presented. By scanning for hatchery...

  19. Strep-Tagged Protein Purification.

    Science.gov (United States)

    Maertens, Barbara; Spriestersbach, Anne; Kubicek, Jan; Schäfer, Frank

    2015-01-01

    The Strep-tag system can be used to purify recombinant proteins from any expression system. Here, protocols for lysis and affinity purification of Strep-tagged proteins from E. coli, baculovirus-infected insect cells, and transfected mammalian cells are given. Depending on the amount of Strep-tagged protein in the lysate, a protocol for batch binding and subsequent washing and eluting by gravity flow can be used. Agarose-based matrices with the coupled Strep-Tactin ligand are the resins of choice, with a binding capacity of up to 9 mg ml(-1). For purification of lower amounts of Strep-tagged proteins, the use of Strep-Tactin magnetic beads is suitable. In addition, Strep-tagged protein purification can also be automated using prepacked columns for FPLC or other liquid-handling chromatography instrumentation, but automated purification is not discussed in this protocol. The protocols described here can be regarded as an update of the Strep-Tag Protein Handbook (Qiagen, 2009). © 2015 Elsevier Inc. All rights reserved.

  20. Studies and comparisons of two photon-tagging systems for the production of monochromatic photon beams for photonuclear experiments

    International Nuclear Information System (INIS)

    Aniel, Thierry.

    1982-06-01

    The performance of photon beams obtained by two different tagging processes (tagging of ''hard'' annihilation photons with ''soft'' associated photons, tagging of bremstrahlung photons with associated electrons) on the same facility was studied. The two processes are described and experimental results on the characteristics of the resulting beams given. The respective advantages of both methods are compared with one another and with those of a quasi-monochromatic beam obtained by the in-flight annihilation of a positron beam. A development based on the second process is then studied together with its applications to photonuclear physics [fr

  1. Development of Sorting System for Fishes by Feed-forward Neural Networks Using Rotation Invariant Features

    Science.gov (United States)

    Shiraishi, Yuhki; Takeda, Fumiaki

    In this research, we have developed a sorting system for fishes, which is comprised of a conveyance part, a capturing image part, and a sorting part. In the conveyance part, we have developed an independent conveyance system in order to separate one fish from an intertwined group of fishes. After the image of the separated fish is captured in the capturing part, a rotation invariant feature is extracted using two-dimensional fast Fourier transform, which is the mean value of the power spectrum with the same distance from the origin in the spectrum field. After that, the fishes are classified by three-layered feed-forward neural networks. The experimental results show that the developed system classifies three kinds of fishes captured in various angles with the classification ratio of 98.95% for 1044 captured images of five fishes. The other experimental results show the classification ratio of 90.7% for 300 fishes by 10-fold cross validation method.

  2. Integrating a FISH imaging system into the cytology laboratory

    Directory of Open Access Journals (Sweden)

    Denice Smith G

    2010-01-01

    Full Text Available We have implemented an interactive imaging system for the interpretation of UroVysion fluorescence in situ hybridization (FISH to improve throughput, productivity, quality control and diagnostic accuracy. We describe the Duet imaging system, our experiences with implementation, and outline the financial investment, space requirements, information technology needs, validation, and training of cytotechnologists needed to integrate such a system into a cytology laboratory. Before purchasing the imaging system, we evaluated and validated the instrument at our facility. Implementation required slide preparation changes, IT modifications, development of training programs, and revision of job descriptions for cytotechnologists. A darkened room was built to house the automated scanning station and microscope, as well as two imaging stations. IT changes included generation of storage for archival images on the LAN, addition of external hard drives for back-up, and changes to cable connections for communication between remote locations. Training programs for cytotechnologists, and pathologists/fellows/residents were developed, and cytotechnologists were integrated into multiple steps of the process. The imaging system has resulted in increased productivity for pathologists, concomitant with an expanded role of cytotechnologists in multiple critical steps, including FISH, scan setup, reclassification, and initial interpretation.

  3. Uptake of C-14 tagged acetate by rice in a paddy soil-to-rice plant system

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, Nobuyoshi; Tagami, Keiko; Uchida, Shigeo [Research Center for Radiation Protection, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan)

    2014-07-01

    Geological disposal of Transuranic (TRU) waste is planned to avoid radiation exposure to the public. One of the dominant nuclides contributing to the dose from TRU waste is C-14, which is long-lived and has very poor sorption properties on natural geological media. Therefore, there are some concerns regarding possible migration of C-14 to the living environments. For the public health safety, it is necessary to clarify pathways of C-14 to human beings in the environment. Intake of C-14 from food source is one of important pathways. In the present study, we examined transfer of C-14 to various parts of rice plant in a paddy soil-to-rice plant system. Rice seedlings in Wagner pots (n=12) were grown for about two months from 7 May 2012 under natural light. The grown plants were moved to a closed chamber on 5 July 2012. The rice plants were grown without water supply from 5 July 2012, and then one liter of C-14 tagged acetate (1.85 MBq) was supplied to the rice plants in the spiked group (n=8) just once on 9 July 2012. For the rice plants in the control group (n=4), uncontaminated water was supplied. These rice plants were air-dried after a harvest on 23 August 2012 and divided into four parts: white rice, bran, rice husk, and the stem and leaf part. The activities of C-14 in the divided parts and air-dried soil samples were determined with a liquid scintillation counting system. Radiocarbon was detected even in the rice plants of the control group. However, the C-14 activity in the soil of the control group was less than the detection limit (1.0 Bq/g). The C-14 activities for the control group decreased in the order of rice husk, bran, white rice, and the stem and leaf part. The detection of C-14 in the control group may be caused by the release of C-14 tagged carbon dioxide from the spiked group. That is, C-14 tagged acetate was converted to carbon dioxide by microbial activity in the spiked group, and then some of the released carbon dioxide was assimilated into

  4. Tagging behaviour with support from controlled vocabulary

    DEFF Research Database (Denmark)

    Lykke, Marianne; Høj, Anne Lyhne; Madsen, Line Nørgaard

    2011-01-01

    ) and an enhanced tagging system (experimental system) that additionally offers suggestions from the Dewey Decimal Classification system (DDC). In the experimental study twenty-eight political students completed four tagging tasks, each comprising fifteen documents. The focus was to examine how suggestions from...

  5. Rhythms in the endocrine system of fish: a review.

    Science.gov (United States)

    Cowan, Mairi; Azpeleta, Clara; López-Olmeda, Jose Fernando

    2017-12-01

    The environment which living organisms inhabit is not constant and many factors, such as light, temperature, and food availability, display cyclic and predictable variations. To adapt to these cyclic changes, animals present biological rhythms in many of their physiological variables, timing their functions to occur when the possibility of success is greatest. Among these variables, many endocrine factors have been described as displaying rhythms in vertebrates. The aim of the present review is to provide a thorough review of the existing knowledge on the rhythms of the endocrine system of fish by examining the hormones that show rhythmicity, how environmental factors control these rhythms and the variation in the responses of the endocrine system depending on the time of the day. We mainly focused on the hypothalamic-pituitary axis, which can be considered as the master axis of the endocrine system of vertebrates and regulates a great variety of functions, including reproduction, growth, metabolism, energy homeostasis, stress response, and osmoregulation. In addition, the rhythms of other hormones, such as melatonin and the factors, produced in the gastrointestinal system of fish are reviewed.

  6. 21 CFR 866.4700 - Automated fluorescence in situ hybridization (FISH) enumeration systems.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated fluorescence in situ hybridization (FISH... Laboratory Equipment and Reagents § 866.4700 Automated fluorescence in situ hybridization (FISH) enumeration... Hybridization (FISH) Enumeration Systems.” See § 866.1(e) for the availability of this guidance document. [70 FR...

  7. Gillnet Tag Program

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Certain fishery management programs require vessels to obtain gillnet tags to be used with their gillnet gear. Gillnet tag data is a collection of requests and...

  8. North Pacific Albacore Tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conventional tagging data are available from 1971 to 1996. Electronic tagging data are available from 2000 to present. The data are managed by SWFSC in Access...

  9. Tagging vs. Controlled Vocabulary

    DEFF Research Database (Denmark)

    Bogers, Toine; Petras, Vivien

    2015-01-01

    The popularity of social tagging has sparked a great deal of debate on whether tags could replace or improve upon professional metadata as descriptors of books and other information objects. In this paper we present a large-scale empirical comparison of the contributions of individual information...... that tags and controlled vocabulary terms do not actually outperform each other consistently, but seem to provide complementary contributions: some information needs are best addressed using controlled vocabulary terms whereas other are best addressed using tags....

  10. A hash based mutual RFID tag authentication protocol in telecare medicine information system.

    Science.gov (United States)

    Srivastava, Keerti; Awasthi, Amit K; Kaul, Sonam D; Mittal, R C

    2015-01-01

    Radio Frequency Identification (RFID) is a technology which has multidimensional applications to reduce the complexity of today life. Everywhere, like access control, transportation, real-time inventory, asset management and automated payment systems etc., RFID has its enormous use. Recently, this technology is opening its wings in healthcare environments, where potential applications include patient monitoring, object traceability and drug administration systems etc. In this paper, we propose a secure RFID-based protocol for the medical sector. This protocol is based on hash operation with synchronized secret. The protocol is safe against active and passive attacks such as forgery, traceability, replay and de-synchronization attack.

  11. Cutaneous skin tag

    Science.gov (United States)

    Skin tag; Acrochordon; Fibroepithelial polyp ... have diabetes. They are thought to occur from skin rubbing against skin. ... The tag sticks out of the skin and may have a short, narrow stalk connecting it to the surface of the skin. Some skin tags are as long as ...

  12. Towards Universal Semantic Tagging

    NARCIS (Netherlands)

    Abzianidze, Lasha; Bos, Johan

    2017-01-01

    The paper proposes the task of universal semantic tagging---tagging word tokens with language-neutral, semantically informative tags. We argue that the task, with its independent nature, contributes to better semantic analysis for wide-coverage multilingual text. We present the initial version of

  13. A Herbivore Tag-and-Trace System Reveals Contact- and Density-Dependent Repellence of a Root Toxin.

    Science.gov (United States)

    Bont, Zoe; Arce, Carla; Huber, Meret; Huang, Wei; Mestrot, Adrien; Sturrock, Craig J; Erb, Matthias

    2017-03-01

    Foraging behavior of root feeding organisms strongly affects plant-environment-interactions and ecosystem processes. However, the impact of plant chemistry on root herbivore movement in the soil is poorly understood. Here, we apply a simple technique to trace the movement of soil-dwelling insects in their habitats without disturbing or restricting their interactions with host plants. We tagged the root feeding larvae of Melolontha melolontha with a copper ring and repeatedly located their position in relation to their preferred host plant, Taraxacum officinale, using a commercial metal detector. This method was validated and used to study the influence of the sesquiterpene lactone taraxinic acid β-D-glucopyranosyl ester (TA-G) on the foraging of M. melolontha. TA-G is stored in the latex of T. officinale and protects the roots from herbivory. Using behavioral arenas with TA-G deficient and control plants, we tested the impact of physical root access and plant distance on the effect of TA-G on M. melolontha. The larvae preferred TA-G deficient plants to control plants, but only when physical root contact was possible and the plants were separated by 5 cm. Melolontha melolontha showed no preference for TA-G deficient plants when the plants were grown 15 cm apart, which may indicate a trade-off between the cost of movement and the benefit of consuming less toxic food. We demonstrate that M. melolontha integrates host plant quality and distance into its foraging patterns and suggest that plant chemistry affects root herbivore behavior in a plant-density dependent manner.

  14. Underwater electric field detection system based on weakly electric fish

    Science.gov (United States)

    Xue, Wei; Wang, Tianyu; Wang, Qi

    2018-04-01

    Weakly electric fish sense their surroundings in complete darkness by their active electric field detection system. However, due to the insufficient detection capacity of the electric field, the detection distance is not enough, and the detection accuracy is not high. In this paper, a method of underwater detection based on rotating current field theory is proposed to improve the performance of underwater electric field detection system. First of all, we built underwater detection system based on the theory of the spin current field mathematical model with the help of the results of previous researchers. Then we completed the principle prototype and finished the metal objects in the water environment detection experiments, laid the foundation for the further experiments.

  15. Prevalence of Clonorchis sinensis Metacercariae in Fish from Water Systems of Seomjin-gang (River).

    Science.gov (United States)

    Sohn, Woon-Mok; Na, Byoung-Kuk; Cho, Shin-Hyeong; Park, Mi-Yeoun; Kim, Cheon-Hyeon; Hwang, Min-Ah; No, Kyeong-Woo; Yoon, Ki-Bok; Lim, Hyun-Cheol

    2017-06-01

    The prevalence of Clonorchis sinensis metacercariae ( Cs Mc) was examined in freshwater fish from the water systems of Seomjin-gang (River), the Republic of Korea. Total 1,604 fish from 7 local sites of Seomjin-gang were examined by artificial digestion methods. The metacercariae of C. sinensis were detected in 102 (39.8%) out of 256 fish (14 species) from the upper reaches of Seomjin-gang, i.e., Osucheon (22.3% in 6 fish species) in Imsil-gun, and Seomjin-gang (63.9% in 9 fish species) in Sunchang-gun, Jeollabuk-do. Their average density was 9.0 per infected fish. They were also found in 132 (48.0%) out of 275 fish (12 spp.) from the middle reaches of Seomjin-gang, i.e., Songdaecheon (58.9% in 4 fish species) in Namwon-si, Jeollabuk-do, and Seomjin-gang (45.2% in 10 fish species) in Gokseong-gun, Jeollanam-do. Their average density was 21.0 per infected fish. Cs Mc were detected in 77 (56.6%) out of 136 fish (11 species) from the lower reaches of Seomjin-gang, i.e., Seomjin-gang (73.3% in 11 fish species) in Gurye-gun, Jeollanam-do, and Namsancheon (8.6% in 1 fish species) in Hadong-gun, Gyeongsangnam-do. Their average density was 64.9 per infected fish. The metacercariae of Metorchis orientalis were also detected in 6 fish species from 4 sites of Seomjin-gang. Conclusively, it has been confirmed that Cs Mc are more or less prevalent in fish from some water systems of Seomjin-gang in Korea.

  16. LHCb Tag Collector

    International Nuclear Information System (INIS)

    Fernández, Paloma Fuente; Clemencic, Marco; Cousin, Nicolas

    2011-01-01

    The LHCb physics software consists of hundreds of packages, each of which is developed by one or more physicists. When the developers have some code changes that they would like released, they commit them to the version control system, and enter the revision number into a database. These changes have to be integrated into a new release of each of the physics analysis applications. Tests are then performed by a nightly build system, which rebuilds various configurations of the whole software stack and executes a suite of run-time functionality tests. A Tag Collector system has been developed using solid standard technologies to cover both the use cases of developers and integration managers. A simple Web interface, based on an AJAX-like technology, is available. Integration with SVN and Nightly Build System, is possible via a Python API. Data are stored in a relational database with the help of an ORM (Object-Relational Mapping) library.

  17. A systems engineering approach to implementation of safety management systems in the Norwegian fishing fleet

    International Nuclear Information System (INIS)

    McGuinness, Edgar; Utne, Ingrid B.

    2014-01-01

    The fishing industry is plagued by a long history of fatality and injury occurrence. Commercial fishing is hence recognized as the most dangerous and difficult of professional callings, in all jurisdictions. Fishing vessels have their own unique set of hazards, a myriad collection of complex occupational accident potentials, barely controlled, co-existing in a perilous work environment. The work in this article is directed by the Norwegian Systematic Health, Environmental and Safety Activities in Enterprises (1997) (Internal Control Regulations [1]), the ISM Code [2] for vessels and their recent applicability to the fishing fleet of Norway. Both safety management works place requirements on the vessel operators and crew to actively manage safety as an on-going concern. The application of these safety management system (SMS) control documents to fishing vessels is just the latest instalment in a continual drive to improve safety in this sector. The difficulty is that there has been no previous systematic approach to safety within the fishing fleet. This article uses the tenants of systems engineering to determine the requirements for such a SMS, detailing the limiting factors and restrictive issues of this complex operating environment. - Highlights: • Systems engineer is applied as a tool for determining requirements for design and construction of a safety management system (SMS). • Outlining a simplistic format, identifying, designingand facilitating improvement opportunities in the conduction and application of SMS’s on fishing vessels. • Knowledge provision is a key requirement of management systems, through provision of understanding, detail orientation and applicable skills for realization. • Outlining, what is to be done and how it is to be completed to accomplish compliance with pertinent legislative requirements. • Promoting a combination of documentation and communication arrangements by which the actionsnecessary for management can be

  18. An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies.

    Science.gov (United States)

    Frentiu, Francesca D; Adamski, Marcin; McGraw, Elizabeth A; Blows, Mark W; Chenoweth, Stephen F

    2009-01-21

    The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selection and traits involved in climatic adaptation along latitudinal gradients. Understanding the molecular genetic basis of such traits has been limited by a lack of genomic resources for this species. Here, we present the first expressed sequence tag (EST) collection for D. serrata that will enable the identification of genes underlying sexually-selected phenotypes and physiological responses to environmental change and may help resolve controversial phylogenetic relationships within the montium subgroup. A normalized cDNA library was constructed from whole fly bodies at several developmental stages, including larvae and adults. Assembly of 11,616 clones sequenced from the 3' end allowed us to identify 6,607 unique contigs, of which at least 90% encoded peptides. Partial transcripts were discovered from a variety of genes of evolutionary interest by BLASTing contigs against the 12 Drosophila genomes currently sequenced. By incorporating into the cDNA library multiple individuals from populations spanning a large portion of the geographical range of D. serrata, we were able to identify 11,057 putative single nucleotide polymorphisms (SNPs), with 278 different contigs having at least one "double hit" SNP that is highly likely to be a real polymorphism. At least 394 EST-associated microsatellite markers, representing 355 different contigs, were also found, providing an additional set of genetic markers. The assembled EST library is available online at http://www.chenowethlab.org/serrata/index.cgi. We have provided the first gene collection and largest set of polymorphic genetic markers, to date, for the fly D. serrata. The EST collection will provide much needed genomic resources for

  19. Optimal use of tandem biotin and V5 tags in ChIP assays

    Directory of Open Access Journals (Sweden)

    Krpic Sanja

    2009-02-01

    Full Text Available Abstract Background Chromatin immunoprecipitation (ChIP assays coupled to genome arrays (Chip-on-chip or massive parallel sequencing (ChIP-seq lead to the genome wide identification of binding sites of chromatin associated proteins. However, the highly variable quality of antibodies and the availability of epitopes in crosslinked chromatin can compromise genomic ChIP outcomes. Epitope tags have often been used as more reliable alternatives. In addition, we have employed protein in vivo biotinylation tagging as a very high affinity alternative to antibodies. In this paper we describe the optimization of biotinylation tagging for ChIP and its coupling to a known epitope tag in providing a reliable and efficient alternative to antibodies. Results Using the biotin tagged erythroid transcription factor GATA-1 as example, we describe several optimization steps for the application of the high affinity biotin streptavidin system in ChIP. We find that the omission of SDS during sonication, the use of fish skin gelatin as blocking agent and choice of streptavidin beads can lead to significantly improved ChIP enrichments and lower background compared to antibodies. We also show that the V5 epitope tag performs equally well under the conditions worked out for streptavidin ChIP and that it may suffer less from the effects of formaldehyde crosslinking. Conclusion The combined use of the very high affinity biotin tag with the less sensitive to crosslinking V5 tag provides for a flexible ChIP platform with potential implications in ChIP sequencing outcomes.

  20. Optimal use of tandem biotin and V5 tags in ChIP assays

    Science.gov (United States)

    Kolodziej, Katarzyna E; Pourfarzad, Farzin; de Boer, Ernie; Krpic, Sanja; Grosveld, Frank; Strouboulis, John

    2009-01-01

    Background Chromatin immunoprecipitation (ChIP) assays coupled to genome arrays (Chip-on-chip) or massive parallel sequencing (ChIP-seq) lead to the genome wide identification of binding sites of chromatin associated proteins. However, the highly variable quality of antibodies and the availability of epitopes in crosslinked chromatin can compromise genomic ChIP outcomes. Epitope tags have often been used as more reliable alternatives. In addition, we have employed protein in vivo biotinylation tagging as a very high affinity alternative to antibodies. In this paper we describe the optimization of biotinylation tagging for ChIP and its coupling to a known epitope tag in providing a reliable and efficient alternative to antibodies. Results Using the biotin tagged erythroid transcription factor GATA-1 as example, we describe several optimization steps for the application of the high affinity biotin streptavidin system in ChIP. We find that the omission of SDS during sonication, the use of fish skin gelatin as blocking agent and choice of streptavidin beads can lead to significantly improved ChIP enrichments and lower background compared to antibodies. We also show that the V5 epitope tag performs equally well under the conditions worked out for streptavidin ChIP and that it may suffer less from the effects of formaldehyde crosslinking. Conclusion The combined use of the very high affinity biotin tag with the less sensitive to crosslinking V5 tag provides for a flexible ChIP platform with potential implications in ChIP sequencing outcomes. PMID:19196479

  1. Systemic colonization of potato plants by a soil-borne, GFP-tagged strain of Dickeya sp. Biovar 3

    NARCIS (Netherlands)

    Czajkowski, R.L.; Boer, de W.; Velvis, H.; Wolf, van der J.M.

    2010-01-01

    Colonization of potato plants by soilborne, green fluorescent protein (GFP)-tagged Dickeya sp. IPO2254 was investigated by selective plating, epifluorescence stereo microscopy (ESM), and confocal laser scanning microscopy (CLSM). Replicated experiments were carried out in a greenhouse using plants

  2. A new signal development process and sound system for diverting fish from water intakes

    International Nuclear Information System (INIS)

    Klinet, D.A.; Loeffelman, P.H.; van Hassel, J.H.

    1992-01-01

    This paper reports that American Electric Power Service Corporation has explored the feasibility of using a patented signal development process and underwater sound system to divert fish away from water intake areas. The effect of water intakes on fish is being closely scrutinized as hydropower projects are re-licensed. The overall goal of this four-year research project was to develop an underwater guidance system which is biologically effective, reliable and cost-effective compared to other proposed methods of diversion, such as physical screens. Because different fish species have various listening ranges, it was essential to the success of this experiment that the sound system have a great amount of flexibility. Assuming a fish's sounds are heard by the same kind of fish, it was necessary to develop a procedure and acquire instrumentation to properly analyze the sounds that the target fish species create to communicate and any artificial signals being generated for diversion

  3. Protein Delivery System Containing a Nickel-Immobilized Polymer for Multimerization of Affinity-Purified His-Tagged Proteins Enhances Cytosolic Transfer.

    Science.gov (United States)

    Postupalenko, Viktoriia; Desplancq, Dominique; Orlov, Igor; Arntz, Youri; Spehner, Danièle; Mely, Yves; Klaholz, Bruno P; Schultz, Patrick; Weiss, Etienne; Zuber, Guy

    2015-09-01

    Recombinant proteins with cytosolic or nuclear activities are emerging as tools for interfering with cellular functions. Because such tools rely on vehicles for crossing the plasma membrane we developed a protein delivery system consisting in the assembly of pyridylthiourea-grafted polyethylenimine (πPEI) with affinity-purified His-tagged proteins pre-organized onto a nickel-immobilized polymeric guide. The guide was prepared by functionalization of an ornithine polymer with nitrilotriacetic acid groups and shown to bind several His-tagged proteins. Superstructures were visualized by electron and atomic force microscopy using 2 nm His-tagged gold nanoparticles as probes. The whole system efficiently carried the green fluorescent protein, single-chain antibodies or caspase 3, into the cytosol of living cells. Transduction of the protease caspase 3 induced apoptosis in two cancer cell lines, demonstrating that this new protein delivery method could be used to interfere with cellular functions. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Meat and Fish Freshness Inspection System Based on Odor Sensing

    OpenAIRE

    Hasan, Najam ul; Ejaz, Naveed; Ejaz, Waleed; Kim, Hyung Seok

    2012-01-01

    We propose a method for building a simple electronic nose based on commercially available sensors used to sniff in the market and identify spoiled/contaminated meat stocked for sale in butcher shops. Using a metal oxide semiconductor-based electronic nose, we measured the smell signature from two of the most common meat foods (beef and fish) stored at room temperature. Food samples were divided into two groups: fresh beef with decayed fish and fresh fish with decayed beef. The prime objective...

  5. Tags on healthcare information websites

    DEFF Research Database (Denmark)

    Lykke, Marianne; Ådland, Marit Kristine

    2018-01-01

    This paper explores tags and tagging behaviour on health information websites using an empirical, user-oriented, exploratory case study. Taggers and editors were interviewed about tags and tagging, while taggers solved tasks that included applying tags to a website. This qualitative data...... articles, request information, and value article content. Some of these show that tags are not only not only topical descriptions, but communicative by intent. This result can potentially inform the design of tagging features....

  6. DNA-tagged Microparticles for Tracing Water Flows and Travel Times in Natural Systems: The First results from Controlled Laboratory Experiments

    Science.gov (United States)

    Bogaard, T.; Bandyopadhyay, S.; Foppen, J. W.

    2017-12-01

    Societal demand for water safety is continuously increasing, being it resilient against flood/droughts, clean water for ecosystems, recreation or safe drinking water. Robust methods to measure temporal and spatial patterns of water and contaminant pathways are still lacking. Our research project aims to develop and apply (1) innovative, robust, and environmental-friendly silica-protected iron oxide micro-particles tagged with artificial DNA to trace contaminant movement and travel times of water in natural systems and (2) an innovative coupled model approach to capture dynamics in hydrological pathways and their effects on water quality. The exceptional property of DNA-tagging is the infinite number of unique tracers that can be produced and their detectability at extreme low concentrations. The advantage of the iron-core of the particle is the magnetic harvesting of the particles from water-samples. Such tracers are thought to give the water sector a unique tool for in-situ mapping of transport of contaminants and pathogenic microorganisms in water systems. However, the characteristics of the particle like magnetic property of the iron-core and surface potential of the silica layer, are of key importance for the behaviour of the particle in surface water and in soils. Furthermore, the application of such micro-particles requires strict protocols for the experiment, sampling and laboratory handling which are currently not available. We used two different types of silica-protected DNA-tagged micro-particles. We performed batch, column and flow experiments to assess the behaviour of the particles. We will present the first results of the controlled laboratory experiments for hydrological tracing. We will discuss the results and link it to the differences in particles design. Furthermore, we will draw conclusions and discuss knowledge gaps for future application of silica-protected DNA-tagged micro-particles in hydrological research.

  7. Effects of passive integrated transponder tags on survival and growth of juvenile Atlantic salmon Salmo salar

    DEFF Research Database (Denmark)

    Larsen, Martin Hage; Thorn, Aske N.; Skov, Christian

    2013-01-01

    Background: A laboratory experiment was conducted to assess the potential impacts of surgically implanted 23 and 32 mm passive integrated transponder (PIT) tags on survival, growth, and body condition of juvenile Atlantic salmon Salmo salar. Rate of tag retention and healing of the tagging incision...... were also evaluated. Atlantic salmon of three different size classes (I: 80 to 99 mm fork length (FL), II: 100 to 119 mm FL, III: 120 to 135 mm FL) were allocated to each of five experimental treatment groups: control, sham-operated (surgery without PIT-tag implantation), 23 mm PIT-tag implantation...... with and without suture closure of the incision, and 32 mm PIT-tag implantation without suture closure. Results: Over the 35-day experiment, mortality occurred only among fish tagged with 32 mm PIT tags (14%) and all fish larger than 103 mm FL survived. Non-sutured Atlantic salmon between 80 and 99 mm FL implanted...

  8. Factors affecting farmers' adoption of integrated rice-fish farming systems in the Mekong delta, Vietnam

    NARCIS (Netherlands)

    Bosma, R.H.; Nhan, D.K.; Udo, H.M.J.; Kaymak, U.

    2012-01-01

    This study investigated the determinants of the adoption of improved rice–fish farming systems in the Mekong delta to support policy making, agricultural land-use planning and extension of integrated rice–fish farming. Recently these systems have been referred to as adaptations to climate change,

  9. Comparing the hierarchy of author given tags and repository given tags in a large document archive

    Science.gov (United States)

    Tibély, Gergely; Pollner, Péter; Palla, Gergely

    2016-10-01

    Folksonomies - large databases arising from collaborative tagging of items by independent users - are becoming an increasingly important way of categorizing information. In these systems users can tag items with free words, resulting in a tripartite item-tag-user network. Although there are no prescribed relations between tags, the way users think about the different categories presumably has some built in hierarchy, in which more special concepts are descendants of some more general categories. Several applications would benefit from the knowledge of this hierarchy. Here we apply a recent method to check the differences and similarities of hierarchies resulting from tags given by independent individuals and from tags given by a centrally managed repository system. The results from our method showed substantial differences between the lower part of the hierarchies, and in contrast, a relatively high similarity at the top of the hierarchies.

  10. Is there excess argon in the Fish Canyon magmatic system?

    Science.gov (United States)

    Wilkinson, C. M.; Sherlock, S.; Kelley, S. P.; Charlier, B. L.

    2010-12-01

    Some phenocrysts from the Fish Canyon Tuff (San Juan volcanic field, south-western Colorado, USA) have yielded anomalously old 40Ar/39Ar apparent ages and yet the sanidine ages are sufficiently reproducible to allow its use as an international standard. The eruption age of the Fish Canyon tuff has recently been determined by high precision analysis and recalibration of the decay constants based on the sanidine standard at 28.305 ± 0.036 Ma [1], slightly younger than the generally accepted U-Pb age. Previously, minerals from the tuff have been used in various geochronological studies e.g., fission-track; U-Pb; Rb-Sr; K-Ar and 40Ar/39Ar, but U-Pb zircon ages which range 28.37 - 28.61 Ma appear to be older than the sanidine and other minerals, including biotite, yield older ages (27.41 - 28.25 Ma for biotite) [2]. In the Fish Canyon volcanic system, the erupted products are thought to exist in the magma chamber for significant periods prior to eruption [3] and then pass rapidly from a high temperature magmatic environment (where Ar is free to re-equilibrate among the minerals), to effectively being quenched upon eruption (where Ar becomes immobile). Artificially elevated ages, older than eruption age, have been identified in some 40Ar/39Ar geochronological studies (e.g. [4]). These older ages may either reflect; 1) argon accumulation in pheno- or xenocrysts (by radioactive decay of parent 40K), 2) excess argon (40ArE) incorporated into a mineral during crystallisation (via diffusion into the mineral lattice or hosted within fluid or melt inclusions) or 3) inherited radiogenic argon (the dated material contains a component older than the age of eruption) [5]. To better understand the effects of 40ArE on 40Ar/39Ar apparent ages we have conducted a detailed study of intra-grain grain age variations by UV-LAMP Ar-analysis. Analysis of polished thick sections has been performed in-situ using a 213nm laser and Nu Instruments Noblesse which is able to discriminate against

  11. Cost effective system for monitoring of fish migration with a camera

    Science.gov (United States)

    Sečnik, Matej; Brilly, Mitja; Vidmar, Andrej

    2016-04-01

    Within the European LIFE project Ljubljanica connects (LIFE10 NAT/SI/000142) we have developed a cost-effective solution for the monitoring of fish migration through the fish passes with the underwater camera. In the fish pass at Ambrožev trg and in the fish pass near the Fužine castle we installed a video camera called "Fishcam" to be able to monitor the migration of fish through the fish passes and success of its reconstruction. Live stream from fishcams installed in the fishpassesis available on our project website (http://ksh.fgg.uni-lj.si/ljubljanicaconnects/ang/12_camera). The system for the fish monitoring is made from two parts. First is the waterproof box for the computer with charger and the second part is the camera itself. We used a high sensitive Sony analogue camera. The advantage of this camera is that it has very good sensitivity in low light conditions, so it can take good quality pictures even at night with a minimum additional lighting. For the night recording we use additional IR reflector to illuminate passing fishes. The camera is connected to an 8-inch tablet PC. We decided to use a tablet PC because it is quite small, cheap, it is relatively fast and has a low power consumption. On the computer we use software which has advanced motion detection capabilities, so we can also detect the small fishes. When the fish is detected by a software, its photograph is automatically saved to local hard drive and for backup also on Google drive. The system for monitoring of fish migration has turned out to work very well. From the beginning of monitoring in June 2015 to end of the year there were more than 100.000 photographs produced. The first analysis of them was already prepared estimating fish species and their frequency in passing the fish pass.

  12. A herbivore tag-and-trace system reveals contact- and density-dependent repellence of a root toxin

    OpenAIRE

    Bont, Zoe; Arce, Carla; Huber, Meret; Huang, Wei; Mestrot, Adrien; Sturrock, Craig; Erb, Matthais

    2017-01-01

    Foraging behavior of root feeding organisms strongly affects plant-environment-interactions and ecosystem processes. However, the impact of plant chemistry on root herbivore movement in the soil is poorly understood. Here, we apply a simple technique to trace the movement of soil-dwelling insects in their habitats without disturbing or restricting their interactions with host plants. We tagged the root feeding larvae of Melolontha melolontha with a copper ring and repeatedly located their pos...

  13. An active ac/ds transposon system for activation tagging in tomato cultivar m82 using clonal propagation.

    Science.gov (United States)

    Carter, Jared D; Pereira, Andy; Dickerman, Allan W; Veilleux, Richard E

    2013-05-01

    Tomato (Solanum lycopersicum) is a model organism for Solanaceae in both molecular and agronomic research. This project utilized Agrobacterium tumefaciens transformation and the transposon-tagging construct Activator (Ac)/Dissociator (Ds)-ATag-Bar_gosGFP to produce activation-tagged and knockout mutants in the processing tomato cultivar M82. The construct carried hygromycin resistance (hyg), green fluorescent protein (GFP), and the transposase (TPase) of maize (Zea mays) Activator major transcript X054214.1 on the stable Ac element, along with a 35S enhancer tetramer and glufosinate herbicide resistance (BAR) on the mobile Ds-ATag element. An in vitro propagation strategy was used to produce a population of 25 T0 plants from a single transformed plant regenerated in tissue culture. A T1 population of 11,000 selfed and cv M82 backcrossed progeny was produced from the functional T0 line. This population was screened using glufosinate herbicide, hygromycin leaf painting, and multiplex polymerase chain reaction (PCR). Insertion sites of transposed Ds-ATag elements were identified through thermal asymmetric interlaced PCR, and resulting product sequences were aligned to the recently published tomato genome. A population of 509 independent, Ds-only transposant lines spanning all 12 tomato chromosomes has been developed. Insertion site analysis demonstrated that more than 80% of these lines harbored Ds insertions conducive to activation tagging. The capacity of the Ds-ATag element to alter transcription was verified by quantitative real-time reverse transcription-PCR in two mutant lines. The transposon-tagged lines have been immortalized in seed stocks and can be accessed through an online database, providing a unique resource for tomato breeding and analysis of gene function in the background of a commercial tomato cultivar.

  14. ConiferEST: an integrated bioinformatics system for data reprocessing and mining of conifer expressed sequence tags (ESTs

    Directory of Open Access Journals (Sweden)

    Carter Kikia

    2007-05-01

    Full Text Available Abstract Background With the advent of low-cost, high-throughput sequencing, the amount of public domain Expressed Sequence Tag (EST sequence data available for both model and non-model organism is growing exponentially. While these data are widely used for characterizing various genomes, they also present a serious challenge for data quality control and validation due to their inherent deficiencies, particularly for species without genome sequences. Description ConiferEST is an integrated system for data reprocessing, visualization and mining of conifer ESTs. In its current release, Build 1.0, it houses 172,229 loblolly pine EST sequence reads, which were obtained from reprocessing raw DNA sequencer traces using our software – WebTraceMiner. The trace files were downloaded from NCBI Trace Archive. ConiferEST provides biologists unique, easy-to-use data visualization and mining tools for a variety of putative sequence features including cloning vector segments, adapter sequences, restriction endonuclease recognition sites, polyA and polyT runs, and their corresponding Phred quality values. Based on these putative features, verified sequence features such as 3' and/or 5' termini of cDNA inserts in either sense or non-sense strand have been identified in-silico. Interestingly, only 30.03% of the designated 3' ESTs were found to have an authenticated 5' terminus in the non-sense strand (i.e., polyT tails, while fewer than 5.34% of the designated 5' ESTs had a verified 5' terminus in the sense strand. Such previously ignored features provide valuable insight for data quality control and validation of error-prone ESTs, as well as the ability to identify novel functional motifs embedded in large EST datasets. We found that "double-termini adapters" were effective indicators of potential EST chimeras. For all sequences with in-silico verified termini/terminus, we used InterProScan to assign protein domain signatures, results of which are available

  15. ConiferEST: an integrated bioinformatics system for data reprocessing and mining of conifer expressed sequence tags (ESTs).

    Science.gov (United States)

    Liang, Chun; Wang, Gang; Liu, Lin; Ji, Guoli; Fang, Lin; Liu, Yuansheng; Carter, Kikia; Webb, Jason S; Dean, Jeffrey F D

    2007-05-29

    With the advent of low-cost, high-throughput sequencing, the amount of public domain Expressed Sequence Tag (EST) sequence data available for both model and non-model organism is growing exponentially. While these data are widely used for characterizing various genomes, they also present a serious challenge for data quality control and validation due to their inherent deficiencies, particularly for species without genome sequences. ConiferEST is an integrated system for data reprocessing, visualization and mining of conifer ESTs. In its current release, Build 1.0, it houses 172,229 loblolly pine EST sequence reads, which were obtained from reprocessing raw DNA sequencer traces using our software--WebTraceMiner. The trace files were downloaded from NCBI Trace Archive. ConiferEST provides biologists unique, easy-to-use data visualization and mining tools for a variety of putative sequence features including cloning vector segments, adapter sequences, restriction endonuclease recognition sites, polyA and polyT runs, and their corresponding Phred quality values. Based on these putative features, verified sequence features such as 3' and/or 5' termini of cDNA inserts in either sense or non-sense strand have been identified in-silico. Interestingly, only 30.03% of the designated 3' ESTs were found to have an authenticated 5' terminus in the non-sense strand (i.e., polyT tails), while fewer than 5.34% of the designated 5' ESTs had a verified 5' terminus in the sense strand. Such previously ignored features provide valuable insight for data quality control and validation of error-prone ESTs, as well as the ability to identify novel functional motifs embedded in large EST datasets. We found that "double-termini adapters" were effective indicators of potential EST chimeras. For all sequences with in-silico verified termini/terminus, we used InterProScan to assign protein domain signatures, results of which are available for in-depth exploration using our biologist

  16. PIT Tagging Anurans

    Science.gov (United States)

    McCreary, Brome

    2008-01-01

    The following video demonstrates a procedure to insert a passive integrated transponder (PIT) tag under the skin of an anuran (frog or toad) for research and monitoring purposes. Typically, a 12.5 mm tag (0.5 in.) is used to uniquely identify individual anurans as smal as 40 mm (1.6 in.) in length from snout to vent. Smaller tags are also available and allow smaller anurans to be tagged. The procedure does not differ for other sizes of tages or other sizes of anurans. Anyone using this procedure should ensure that the tag is small enough to fit easily behind the sacral hump of the anuran, as shown in this video.

  17. Use of a parallel artificial membrane system to evaluate passive absorption and elimination in small fish.

    Science.gov (United States)

    Kwon, Jung-Hwan; Katz, Lynn E; Liljestrand, Howard M

    2006-12-01

    A parallel artificial lipid membrane system was developed to mimic passive mass transfer of hydrophobic organic chemicals in fish. In this physical model system, a membrane filter-supported lipid bilayer separates two aqueous phases that represent the external and internal aqueous environments of fish. To predict bioconcentration kinetics in small fish with this system, literature absorption and elimination rates were analyzed with an allometric diffusion model to quantify the mass transfer resistances in the aqueous and lipid phases of fish. The effect of the aqueous phase mass transfer resistance was controlled by adjusting stirring intensity to mimic bioconcentration rates in small fish. Twenty-three simple aromatic hydrocarbons were chosen as model compounds for purposes of evaluation. For most of the selected chemicals, literature absorption/elimination rates fall into the range predicted from measured membrane permeabilities and elimination rates of the selected chemicals determined by the diffusion model system.

  18. Monitoring of Aquatic Biota in Intensive Fish Farming System

    Directory of Open Access Journals (Sweden)

    Lukáš Mareš

    2017-01-01

    Full Text Available The aim of the study was to conduct a detailed research of aquatic invertebrates in the intensive breeding system of salmonid fish in order to determine the taxonomic composition of the community and its seasonal dynamics, and to identify those species that may be hosts of parasitic disease agents. To date, this issue has not been studied at all in the Czech Republic. Monitoring was conducted on the Danish type recirculation system near the municipality of Pravíkov in the Highlands of the Czech Republic from April till November 2015. A total of 9 series of samples were taken. Macrozoobenthos was evaluated in terms of taxonomic composition and abundance. Basic physicochemical properties of water (temperature, oxygen concentration, pH and conductivity were also measured. In total, 69 taxa of aquatic invertebrates were noted, with the wall being the richest with a mean abundance of 756 pcs/m2. Permanent groups predominated; the most numerous group was the subphylum Crustaceae, represented by a single species, Asellus aquticus.

  19. Performance Analysis of Multipurpose Refrigeration System (MRS on Fishing Vessel

    Directory of Open Access Journals (Sweden)

    Ust Y.

    2016-04-01

    Full Text Available The use of efficient refrigerator/freezers helps considerably to reduce the amount of the emitted greenhouse gas. A two-circuit refrigerator-freezer cycle (RF reveals a higher energy saving potential than a conventional cycle with a single loop of serial evaporators, owing to pressure drop in each evaporator during refrigeration operation and low compression ratio. Therefore, several industrial applications and fish storage systems have been utilized by using multipurpose refrigeration cycle. That is why a theoretical performance analysis based on the exergetic performance coefficient, coefficient of performance (COP, exergy efficiency and exergy destruction ratio criteria, has been carried out for a multipurpose refrigeration system by using different refrigerants in serial and parallel operation conditions. The exergetic performance coefficient criterion is defined as the ratio of exergy output to the total exergy destruction rate (or loss rate of availability. According to the results of the study, the refrigerant R32 shows the best performance in terms of exergetic performance coefficient, COP, exergy efficiency, and exergy destruction ratio from among the other refrigerants (R1234yf, R1234ze, R404A, R407C, R410A, R143A and R502. The effects of the condenser, freezer-evaporator and refrigerator-evaporator temperatures on the exergetic performance coefficient, COP, exergy efficiency and exergy destruction ratios have been fully analyzed for the refrigerant R32.

  20. CH4 Emission Model from Bos Primigenius Waste in Fish-Water: Implications for Integrated Livestock-Fish Farming Systems

    Directory of Open Access Journals (Sweden)

    Joshua O. Okeniyi

    2013-07-01

    Full Text Available This paper studies a methane (CH4 emission model from the waste of cattle (B. primigenius based on trends in integrated livestock-fish farming adoption by farmers in Nigeria. Dung of B. primigenius was employed as substrate in fish-water, obtained from a fish-rearing farm, as a matrix medium for simulating a low-oxygen wastewater environment of an agriculture-aquaculture system. A substrate to fish-water mass ratio of 1:3 was used, developed in a laboratory-size digesting reactor system. Volumetric readings, at ambient temperature conditions and with a retention time of thirty-two days, were then subjected to the logistic probability density function, and tested against correlation coefficient and Nash-Sutcliffe coefficient of efficiency criteria. The readings show that a volume of CH4-containing gas as high as 65.3 x 10−3 dm3 was produced on the 13th day from the B. primigenius substrate. Also, production of 234.59 x 10−3 dm3/kg CH4-containing gas, totaling 703.76 x 10−3 dm3, was observed through the studied retention time. The 60% CH4 constituent model of the measured gas generation showed a potency of 2.0664 kg emission per animal, which is equivalent to 43.3944 CO2eq of global warming potential (GWP annually per animal. This bears environmental and climate change implications, and therefore alternative sustainable practices for integrated livestock-fish farming adoption are suggested.

  1. Bioaccumulation of polonium-210 in fish of the Kaveri river system

    International Nuclear Information System (INIS)

    Shaheed, K.; Shahul Hameed, P.; Iyengar, M.A.R.

    1997-01-01

    Concentration of naturally occurring radioactive nuclide polonium-210 was determined in selected species of fish from the Kaveri river system at Tiruchirappalli. It is shown that 210 Po is non-uniformly distributed within these fishes. Concentrations of 210 Po in the muscle of fish ranged from 3.3 to 8.2 Bq/kg (wet weight). Concentration factors of Po 210 in edible portion of fish from river water worked out to be 2.5 x 10 3 to 6.3 x 10 3 . Radiation dose to public due to consumption of fish from the Kaveri river varied from 5.1 to 27.3 μSv/y. The results have implications that fish represents an important source of supply of 210 Po to humans. (author). 16 refs., 2 tabs

  2. Photoperiod effect on commercial fishes cultured in different types of experimental systems

    Directory of Open Access Journals (Sweden)

    Aragón-Flores, E.A.

    2014-07-01

    Full Text Available The influence on the abiotic factors in aquaculture has been widely studied in order to control growing variables. Studies have been made to assess the positive or negative influence of photoperiod in the different life stages of some fish species. The photoperiod can influence feed visualization and social behavior in fishes; these responses influence fish growth and survival. Similarly, photoperiod plays an important role in the release of hormones that stimulate sexual development and reproduction. The aim of this review was to research the influence of photoperiod in different commercial fish species cultured in different types of experimental systems.

  3. Method and apparatus for manufacturing gas tags

    International Nuclear Information System (INIS)

    Gross, K.C.; Laug, M.T.

    1996-01-01

    For use in the manufacture of gas tags employed in a gas tagging failure detection system for a nuclear reactor, a plurality of commercial feed gases each having a respective noble gas isotopic composition are blended under computer control to provide various tag gas mixtures having selected isotopic ratios which are optimized for specified defined conditions such as cost. Using a new approach employing a discrete variable structure rather than the known continuous-variable optimization problem, the computer controlled gas tag manufacturing process employs an analytical formalism from condensed matter physics known as stochastic relaxation, which is a special case of simulated annealing, for input feed gas selection. For a tag blending process involving M tag isotopes with N distinct feed gas mixtures commercially available from an enriched gas supplier, the manufacturing process calculates the cost difference between multiple combinations and specifies gas mixtures which approach the optimum defined conditions. The manufacturing process is then used to control tag blending apparatus incorporating tag gas canisters connected by stainless-steel tubing with computer controlled valves, with the canisters automatically filled with metered quantities of the required feed gases. 4 figs

  4. Parasites as biological tags in marine fisheries research: European Atlantic waters.

    Science.gov (United States)

    Mackenzie, K; Hemmingsen, W

    2015-01-01

    Studies of the use of parasites as biological tags for stock identification and to follow migrations of marine fish, mammals and invertebrates in European Atlantic waters are critically reviewed and evaluated. The region covered includes the North, Baltic, Barents and White Seas plus Icelandic waters, but excludes the Mediterranean and Black Seas. Each fish species or ecological group of species is treated separately. More parasite tag studies have been carried out on Atlantic herring Clupea harengus than on any other species, while cod Gadus morhua have also been the subject of many studies. Other species that have been the subjects of more than one study are: blue whiting Micromesistius poutassou, whiting Merlangius merlangus, haddock Melanogrammus aeglefinus, Norway pout Trisopterus esmarkii, horse mackerel Trachurus trachurus and mackerel Scomber scombrus. Other species are dealt with under the general headings redfishes, flatfish, tunas, anadromous fish, elasmobranchs, marine mammals and invertebrates. A final section highlights how parasites can be, and have been, misused as biological tags, and how this can be avoided. It also reviews recent developments in methodology and parasite genetics, considers the potential effects of climate change on the distributions of both hosts and parasites, and suggests host-parasite systems that should reward further research.

  5. The Effects of Target Audience on Social Tagging

    Science.gov (United States)

    Alsarhan, Hesham

    2013-01-01

    Online social bookmarking systems allow users to assign tags (i.e., keywords) to represent the content of resources. Research on the effects of target audience on social tagging suggests that taggers select different tags for themselves, their community (e.g., family, friends, colleagues), and the general public (Panke & Gaiser, 2009; Pu &…

  6. Exploiting phase measurements of EPC Gen2 RFID tags

    NARCIS (Netherlands)

    Huiting, J.; Flisijn, Hubert; Kokkeler, Andre B.J.; Smit, Gerardus Johannes Maria

    2013-01-01

    This paper presents a 2d localization system for UHF RFID tags. By measuring the phase between the transmitted continuous wave and received backscatter from the tag at different frequencies, it is possible to estimate the distance between the reader and tag. By determining distance estimates to

  7. Change and diversity in smallholder rice-fish systems: Recent evidence and policy lessons from Bangladesh

    NARCIS (Netherlands)

    Dey, M.M.; Spielman, D.J.; Haque, A.B.M.M.; Rahman, M.S.; Valmonte-Santos, R.

    2013-01-01

    Efforts to unlock the genetic potential of both rice and fish, when combined with improvements in the management of rice-fish systems, can potentially increase agricultural productivity and food security in some of the poorest and most populous countries in Asia. In Bangladesh, estimates suggest

  8. Structural and functional effects of heavy metals on the nervous system, including sense organs, of fish

    DEFF Research Database (Denmark)

    Baatrup, E

    1991-01-01

    1. Today, fish in the environment are inevitably exposed to chemical pollution. Although most hazardous substances are present at concentrations far below the lethal level, they may still cause serious damage to the life processes of these animals. 2. Fish depend on an intact nervous system, incl...

  9. Testing resonating vector strength: Auditory system, electric fish, and noise

    Science.gov (United States)

    Leo van Hemmen, J.; Longtin, André; Vollmayr, Andreas N.

    2011-12-01

    Quite often a response to some input with a specific frequency ν○ can be described through a sequence of discrete events. Here, we study the synchrony vector, whose length stands for the vector strength, and in doing so focus on neuronal response in terms of spike times. The latter are supposed to be given by experiment. Instead of singling out the stimulus frequency ν○ we study the synchrony vector as a function of the real frequency variable ν. Its length turns out to be a resonating vector strength in that it shows clear maxima in the neighborhood of ν○ and multiples thereof, hence, allowing an easy way of determining response frequencies. We study this "resonating" vector strength for two concrete but rather different cases, viz., a specific midbrain neuron in the auditory system of cat and a primary detector neuron belonging to the electric sense of the wave-type electric fish Apteronotus leptorhynchus. We show that the resonating vector strength always performs a clear resonance correlated with the phase locking that it quantifies. We analyze the influence of noise and demonstrate how well the resonance associated with maximal vector strength indicates the dominant stimulus frequency. Furthermore, we exhibit how one can obtain a specific phase associated with, for instance, a delay in auditory analysis.

  10. Energy Based Control System Designs for Underactuated Robot Fish Propulsion

    OpenAIRE

    Roper, Daniel

    2013-01-01

    In nature through millions of years of evolution fish and cetaceans have developed fast efficient and highly manoeuvrable methods of marine propulsion. A recent explosion in demand for sub sea robotics, for conducting tasks such as sub sea exploration and survey has left developers desiring to capture some of the novel mechanisms evolved by fish and cetaceans to increase the efficiency of speed and manoeuvrability of sub sea robots. Research has revealed that interactions with...

  11. MARKETING SYSTEM OF MARINE FISH IN BANGLADESH: AN EMPIRICAL STUDY

    OpenAIRE

    Islam, M. Serajul; Miah, Tofazzal Hossain; Haque, Md. Mojammel

    2000-01-01

    This paper was designed to investigate the present status of marine fish marketing aiming to determine marketing costs, margins and profits of marketing intermediaries both in domestic and export marketing. Primary data were collected by survey method wherein various market intermediaries were interviewed from selected districts for eliciting information at various stages of marine fish marketing. The study revealed that marketing margin as well as marketing profit both were relatively higher...

  12. Microplanktonic community structure in a coastal system relative to a Phaeocystis bloom inferred from morphological and tag pyrosequencing methods.

    Directory of Open Access Journals (Sweden)

    Sébastien Monchy

    Full Text Available BACKGROUND: Massive phytoplankton blooms, like the recurrent Phaeocystis proliferation observed every year in the Eastern English Channel (EEC, have a significant influence on the overall planktonic community structure and their food web dynamics. As well as being an important area for local fisheries, the EEC is an ideal ecosystem for work on microbial diversity. This is because, although its environmental context is relatively complex, it is reasonably well understood due to several years of monitoring and morphological observations of its planktonic organisms. The objective of our study was to better understand the under-explored microbial eukaryotic diversity relative to the Phaeocystis bloom. METHODOLOGY AND PRINCIPAL FINDINGS: The community structure of microplankton (diatoms, haptophytes, ciliates and dinoflagellates was studied through morphological observations and tag pyrosequencing. During the annual Phaeocystis spring bloom, the phytoplankton biomass increased by 34-fold, while the microzooplankton biomass showed a 4-fold increase, representing on average about 4.6% of the biomass of their phytoplankton prey. Tag pyrosequencing unveiled an extensive diversity of Gymnodiniaceae, with G. spirale and G. fusiformis representing the most abundant reads. An extended diversity of Phaeocystales, with partial 18S rDNA genes sequence identity as low as 85% was found, with taxa corresponding to P. globosa, but also to unknown Phaeocystaceae. CONCLUSIONS: Morphological analyses and pyrosequencing were generally in accordance with capturing frequency shifts of abundant taxa. Tag pyrosequencing allowed highlighting the maintenance of microplankton diversity during the Phaeocystis bloom and the increase of the taxa presenting low number of reads (minor taxa along with the dominant ones in response to biotic and/or abiotic changing conditions. Although molecular approaches have enhanced our perception on diversity, it has come to light that the

  13. Tracking animals in freshwater with electronic tags: past, present and future

    Science.gov (United States)

    Cooke, Steven J.; Midwood, Jonathan D.; Thiem, Jason D.; Klimley, Peter; Lucas, Martyn C.; Thorstad, Eva B.; Eiler, John; Holbrook, Chris; Ebner, Brendan C.

    2013-01-01

    Considerable technical developments over the past half century have enabled widespread application of electronic tags to the study of animals in the wild, including in freshwater environments. We review the constraints associated with freshwater telemetry and biologging and the technical developments relevant to their use. Technical constraints for tracking animals are often influenced by the characteristics of the animals being studied and the environment they inhabit. Collectively, they influence which and how technologies can be used and their relative effectiveness. Although radio telemetry has historically been the most commonly used technology in freshwater, passive integrated transponder (PIT) technology, acoustic telemetry and biologgers are becoming more popular. Most telemetry studies have focused on fish, although an increasing number have focused on other taxa, such as turtles, crustaceans and molluscs. Key technical developments for freshwater systems include: miniaturization of tags for tracking small-size life stages and species, fixed stations and coded tags for tracking large samples of animals over long distances and large temporal scales, inexpensive PIT systems that enable mass tagging to yield population- and community-level relevant sample sizes, incorporation of sensors into electronic tags, validation of tag attachment procedures with a focus on maintaining animal welfare, incorporation of different techniques (for example, genetics, stable isotopes) and peripheral technologies (for example, geographic information systems, hydroacoustics), development of novel analytical techniques, and extensive international collaboration. Innovations are still needed in tag miniaturization, data analysis and visualization, and in tracking animals over larger spatial scales (for example, pelagic areas of lakes) and in challenging environments (for example, large dynamic floodplain systems, under ice). There seems to be a particular need for adapting

  14. Contribution of emissions to concentrations: the TAGGING 1.0 submodel based on the Modular Earth Submodel System (MESSy 2.52)

    Science.gov (United States)

    Grewe, Volker; Tsati, Eleni; Mertens, Mariano; Frömming, Christine; Jöckel, Patrick

    2017-07-01

    Questions such as what is the contribution of road traffic emissions to climate change? or what is the impact of shipping emissions on local air quality? require a quantification of the contribution of specific emissions sectors to the concentration of radiatively active species and air-quality-related species, respectively. Here, we present a diagnostics package, implemented in the Modular Earth Submodel System (MESSy), which keeps track of the contribution of source categories (mainly emission sectors) to various concentrations. The diagnostics package is implemented as a submodel (TAGGING) of EMAC (European Centre for Medium-Range Weather Forecasts - Hamburg (ECHAM)/MESSy Atmospheric Chemistry). It determines the contributions of 10 different source categories to the concentration of ozone, nitrogen oxides, peroxyacytyl nitrate, carbon monoxide, non-methane hydrocarbons, hydroxyl, and hydroperoxyl radicals ( = tagged tracers). The source categories are mainly emission sectors and some other sources for completeness. As emission sectors, road traffic, shipping, air traffic, anthropogenic non-traffic, biogenic, biomass burning, and lightning are considered. The submodel obtains information on the chemical reaction rates, online emissions, such as lightning, and wash-out rates. It then solves differential equations for the contribution of a source category to each of the seven tracers. This diagnostics package does not feed back to any other part of the model. For the first time, it takes into account chemically competing effects: for example, the competition between NOx, CO, and non-methane hydrocarbons (NMHCs) in the production and destruction of ozone. We show that the results are in-line with results from other tagging schemes and provide plausibility checks for concentrations of trace gases, such as OH and HO2, which have not previously been tagged. The budgets of the tagged tracers, i.e. the contribution from individual source categories (mainly emission

  15. Flavour Tagging at LHCb

    CERN Multimedia

    Grabalosa Gandara, M

    2009-01-01

    To do precise CP violation measurements, the most possible accurate knowledge of the flavour at production of the reconstructed B meson is required. This poster summarizes the flavour tagging performances for the LHCb experiment. We use same side an opposite side algorithms to establish wheter the meson contained a b or a b\\bar quark. The final decision is obtained through a combination of several methods. The use of control channels, decays to a flavour specific final state, will allow to determine the wrong tag fraction \\omega (the probability of a tag to be wrong), which can be used as input for the determination of CKM unitary triangle angles.

  16. Flavour Tagging with the LHCb experiment

    CERN Multimedia

    Birnkraut, Alex

    2015-01-01

    Measurements of flavour oscillations and time-dependent CP asymmetries in neutral B meson systems require knowledge of the b quark production flavour. This identification is performed by the Flavour Tagging.

  17. Distribution of mesopredatory fish determined by habitat variables in a predator-depleted coastal system.

    Science.gov (United States)

    Bergström, Lena; Karlsson, Martin; Bergström, Ulf; Pihl, Leif; Kraufvelin, Patrik

    Shallow nearshore habitats are highly valued for supporting marine ecosystems, but are subject to intense human-induced pressures. Mesopredatory fish are key components in coastal food webs, and alterations in their abundance may have evident effects also on other parts of the ecosystem. The aim of this study was to clarify the relationship between the abundance of coastal mesopredatory fish, defined as mid-trophic level demersal and benthic species with a diet consisting predominantly of invertebrates, and ambient environmental variables in a fjord system influenced by both eutrophication and overfishing. A field survey was conducted over a coastal gradient comprising 300 data points sampled consistently for fish community and environmental data. Results from multivariate and univariate analyses supported each other, demonstrating that mesopredatory fish abundance at species and functional group level was positively related to the cover of structurally complex vegetation and negatively related to eutrophication, as measured by water transparency. Contrary to other studies showing an inverse relationship to piscivore abundance over time, the spatial distribution of mesopredatory fish was not locally regulated by the abundance of piscivorous fish, probably attributed to piscivores being at historically low levels due to previous overfishing. Mesopredatory fish abundance was highest in areas with high habitat quality and positively related to the abundance of piscivores, suggesting a predominance of bottom-up processes. We conclude that, in parallel with ongoing regulations of fishing pressure, measures to restore habitat function and food web productivity are important for the recovery of coastal fish communities in the area.

  18. Partition of selected food preservatives in fish oil-water systems

    DEFF Research Database (Denmark)

    Cheng, Hongyuan; Friis, Alan; Leth, Torben

    2010-01-01

    The partition coefficients (Kow) of benzoic acid and sorbic acid in systems of fish oil (sand eel)–water, fish oil–buffer solution, rape oil–water and olive oil–water were experimentally determined in a temperature range from 5 to 43 °C and pH from 4.5 to 6.5 °C. The dimerization of benzoic acid...... in fish oil–water system was observed at 25 °C. Two modifications have been made to the Nordic Food Analysis Standard for the determination of sorbic acid by HPLC. The experimental results show that the Kow of benzoic acid and sorbic acid in fish oil–buffer system is ca. 100 times lower than that in fish...... oil–water system. The Kow values of benzoic acid and sorbic acid in fish oil and water system decrease with increasing system pH values. The partition coefficients of plant origin and fish origin oils are in the same order of magnitude even though their molecular structures are very different....

  19. Evaluation of a pilot fish handling system at Bruce NGS 'A'

    International Nuclear Information System (INIS)

    Griffiths, J.S.

    1985-10-01

    A pilot fish recovery system using a Hidrostal fish pump was tested in the Bruce NGS 'A' forebay during June, 1984. Despite low forebay fish concentrations, the system was capable of capturing 97,000 alewife/day (3900 kg) if operated continuously. Post-pumping survival averaged 97%. It is estimated that a single pump could handle alewife runs in the 40,000 to 70,000 kg range, but multiple pumps or a single larger pump would be required to assure station protection from the largest runs (>100,000 kg). Results indicate that tank/trailer return of pumped fish is feasible, but other alternatives for returning fish to Lake Huron are also being considered

  20. Distribution and Bioaccumulation of I-131 Within The Water-Fish System

    International Nuclear Information System (INIS)

    Darussalam, M; Wijaya, DGO; Sutrisno

    1996-01-01

    Distribution and Bioaccumulation of I-131 Within The Water-Fish System. As one of fission products, radioiodine I-131 potentially become a pollutant either resulted froma fallout or radioactive waste. Therefore, special interest has been given to handle I-131 starting from its production implementation and its waste management. The observations in this research have been focussed on distribution and bio accumulation of I-131 within the water-fish systems. Some number of Tilapia fish were put in aquaria containing I-131 contamined water with certain radioactivity concentration. Within time interval of 0, 6, 24, 48 and 72 hours after treatment the radioactivities of water media. fish and their organs have been measured. The results show that the radioactivity percentage different water media containing different I-131 concentration tend to have similar patterns. Meanwhile, the I-131 concentrations of fish and their organs were varied with similar patterns for different I-131 content in water media

  1. A New Operating System for Security Tagged Architecture Hardware in Support of Multiple Independent Levels of Security (MILS) Compliant System

    Science.gov (United States)

    2014-04-01

    of services that includes multitasking , inter-task communication, and dynamic memory allocation. The bulk of RTEMS is written in both the Ada and C...rtems_initialize_start_multitasking initiates multitasking and applications can start executing. Approved for Public Release; Distribution Unlimited. 29 3.2.2 Problems in RTEMS...or email address. 6. Security Misconfiguration - Security misconfiguration occurs when the attacker gains unauthorized access because the system

  2. Influence of Melatonin on the Immune System of Fish: A Review

    Science.gov (United States)

    Esteban, M. Ángeles; Cuesta, Alberto; Chaves-Pozo, Elena; Meseguer, José

    2013-01-01

    Endocrine-immune system interactions have been widely demonstrated in mammals, whereas in fish, these relationships remain unclear. Of the organs that constitute the endocrine system, the pineal gland and its secretory product melatonin act in the synchronization of daily and seasonal rhythms in most vertebrates, including fish. Seasonal differences in immunocompetence and disease prevalence have been well documented in humans. Seasonality also strongly influences the life history of fish by controlling the timing of physiological events, such as reproduction, food intake, locomotor activity, and growth performance. Apart from its synchronizing capabilities, the role of melatonin in physiological processes in fish is not thoroughly understood. The purpose of this review is to summarize current studies on the effects of melatonin on the fish immune system. These studies suggest that melatonin represents an important component of fish endocrine-immune system interactions. The elucidation of the defense mechanisms of fish will facilitate the development of health management tools to support the growing finfish aquaculture industry as well as address questions concerning the origins and evolution of the immune system in vertebrates. PMID:23579958

  3. Tagged Vector Contour (TVC)

    Data.gov (United States)

    Kansas Data Access and Support Center — The Kansas Tagged Vector Contour (TVC) dataset consists of digitized contours from the 7.5 minute topographic quadrangle maps. Coverage for the state is incomplete....

  4. To develop a flying fish egg inspection system by a digital imaging base system

    Science.gov (United States)

    Chen, Chun-Jen; Jywe, Wenyuh; Hsieh, Tung-Hsien; Chen, Chien Hung

    2015-07-01

    This paper develops an automatic optical inspection system for flying fish egg quality inspection. The automatic optical inspection system consists of a 2-axes stage, a digital camera, a lens, a LED light source, a vacuum generator, a tube and a tray. This system can automatically find the particle on the flying egg tray and used stage to driver the tube onto the particle. Then use straw and vacuum generator to pick up the particle. The system pick rate is about 30 particles per minute.

  5. Meat and fish freshness inspection system based on odor sensing.

    Science.gov (United States)

    Najam ul Hasan; Ejaz, Naveed; Ejaz, Waleed; Kim, Hyung Seok

    2012-11-09

    We propose a method for building a simple electronic nose based on commercially available sensors used to sniff in the market and identify spoiled/contaminated meat stocked for sale in butcher shops. Using a metal oxide semiconductor-based electronic nose, we measured the smell signature from two of the most common meat foods (beef and fish) stored at room temperature. Food samples were divided into two groups: fresh beef with decayed fish and fresh fish with decayed beef. The prime objective was to identify the decayed item using the developed electronic nose. Additionally, we tested the electronic nose using three pattern classification algorithms (artificial neural network, support vector machine and k-nearest neighbor), and compared them based on accuracy, sensitivity, and specificity. The results demonstrate that the k-nearest neighbor algorithm has the highest accuracy.

  6. Meat and Fish Freshness Inspection System Based on Odor Sensing

    Directory of Open Access Journals (Sweden)

    Hyung Seok Kim

    2012-11-01

    Full Text Available We propose a method for building a simple electronic nose based on commercially available sensors used to sniff in the market and identify spoiled/contaminated meat stocked for sale in butcher shops. Using a metal oxide semiconductor-based electronic nose, we measured the smell signature from two of the most common meat foods (beef and fish stored at room temperature. Food samples were divided into two groups: fresh beef with decayed fish and fresh fish with decayed beef. The prime objective was to identify the decayed item using the developed electronic nose. Additionally, we tested the electronic nose using three pattern classification algorithms (artificial neural network, support vector machine and k-nearest neighbor, and compared them based on accuracy, sensitivity, and specificity. The results demonstrate that the k-nearest neighbor algorithm has the highest accuracy.

  7. Current knowledge on the melatonin system in teleost fish.

    Science.gov (United States)

    Falcón, J; Migaud, H; Muñoz-Cueto, J A; Carrillo, M

    2010-02-01

    Melatonin is a much conserved feature in vertebrates that plays a central role in the entrainment of daily and annual physiological rhythms. Investigations aiming at understanding how melatonin mediates the effects of photoperiod on crucial functions and behaviors have been very active in the last decades, particularly in mammals. In fish a clear-cut picture is still missing. Here we review the available data on (i) the sites of melatonin production in fish, (ii) the mechanisms that control its daily and annual rhythms of production and (iii) the characterization of its different receptor subtypes, their location and regulation. The in vivo and in vitro data on melatonin effects on crucial neuroendocrine regulations, including reproduction, growth, feeding and behavioral responses, are also reviewed. Finally we discuss how manipulation of the photic cues impact on fish circannual clock and annual cycle of reproduction, and how this can be used for aquaculture purposes. Copyright 2009 Elsevier Inc. All rights reserved.

  8. Synthesis of Sensor Fish Data for Assessment of Fish Passage Conditions at Turbines, Spillways, and Bypass Facilities – Phase 1: The Dalles Dam Spillway Case Study

    Energy Technology Data Exchange (ETDEWEB)

    Deng, Zhiqun; Serkowski, John A.; Fu, Tao; Carlson, Thomas J.; Richmond, Marshall C.

    2007-12-31

    This report summarizes the characterization of spillway passage conditions at The Dalles Dam in 2006 and the effort to complete a comprehensive database for data sets from The Dalles Dam spillway Sensor Fish and balloon-tagged live fish experiments. Through The Dalles Dam spillway case study, Pacific Northwest National Laboratory (PNNL) researchers evaluated the database as an efficient means for accessing and retrieving system-wide data for the U.S Army Corps of Engineers (USACE).

  9. ROV-based Underwater Vision System for Intelligent Fish Ethology Research

    Directory of Open Access Journals (Sweden)

    Rui Nian

    2013-09-01

    Full Text Available Fish ethology is a prospective discipline for ocean surveys. In this paper, one ROV-based system is established to perform underwater visual tasks with customized optical sensors installed. One image quality enhancement method is first presented in the context of creating underwater imaging models combined with homomorphic filtering and wavelet decomposition. The underwater vision system can further detect and track swimming fish from the resulting images with the strategies developed using curve evolution and particular filtering, in order to obtain a deeper understanding of fish behaviours. The simulation results have shown the excellent performance of the developed scheme, in regard to both robustness and effectiveness.

  10. A Summary of Fish Data in Six Reaches of The Upper Mississippi River System

    National Research Council Canada - National Science Library

    Gutreuter, Steve

    1997-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 1,994 collections of fishes from stratified random and permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during 1993...

  11. Microbial abundance and diversity in water, and immune parameters of red tilapia reared in bioflocs system with different fish density (25 fish/m3, 50 fish/m3, and 100 fish/m3

    Directory of Open Access Journals (Sweden)

    Frid Agustinus

    2010-07-01

    Full Text Available ABSTRACTThe objective of this experiment was to study microbial abundance and diversity in the water, and immune parameters of red tilapia Oreochromis sp. cultured in bioflok system with different fish stocking densities. The experiment comprised of two different factors, carbon source addition (bioflocs and control, and fish stocking density (25 fish/m3, 50 fish/m3, dan 100 fish/m3, with an experimental period of 99 days. Microbial load in water was determined biweekly, whereas immune parameters represented by fish blood profile were measured on day 0, 50, and 90. There was no significant difference in total bacteria count in the water of all treatments; there was however a tendency shown by all treatments that the microbial load in water increased along with the culture period. There were 4 genera of bacteria which particularly found in bioflok system, which are Acinetobacter sp., Corynobacterium sp., Listeria sp., dan Pseudomonas sp, and are suggested to play a role in bioflok formation. The percentage of phagocytic index of fish in bioflok system was higher than that in control, and may indicate that bioflok may stimulate the fish immune system.Keywords: bioflocs, red tilapia, bacteria, blood profile. ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi kelimpahan dan keragaman jenis bakteri dalam air dan parameter imunitas ikan nila Oreochromis sp. yang dipelihara dalam sistem bioflok dengan kepadatan ikan yang berbeda. Penelitian terdiri atas dua faktor perlakuan yaitu penambahan sumber carbon (bioflok dan kontrol, dan padat penebaran ikan (25 ekor/m3, 50 ekor/m3, dan 100 ekor/m3 dengan lama waktu pemeliharaan ikan selama 99 hari. Kelimpahan bakteri diukur setiap 2 minggu sekali selama masa pemeliharaan. Parameter imunitas meliputi gambaran darah diukur dengan pengambilan contoh darah yang dilakukan pada tiga ekor ikan pada hari ke 0, 50, dan 99. Kelimpahan bakteri pada semua perlakuan pada setiap titik pengamatan tidak menunjukkan

  12. The Canal da Piracema at Itaipu Dam as a fish pass system

    Directory of Open Access Journals (Sweden)

    Sérgio Makrakis

    Full Text Available The Canal da Piracema is the longest (nearly 10 km fish pass system in the world. The construction of this fish pass was somehow controversial, because it connected two distinct ichthyofaunistic provinces. This study evaluated the ichthyofauna present in the Canal da Piracema and the abundance and distribution of long-distance migratory fish species along this fish pass system (evaluated possible selectivity. The Canal da Piracema was shown to be difficult to sample because of its environmental heterogeneity: artificial ponds, ladders and nature-like fish pass. To solve this problem, we used several fishing gears, adequate for the several biotopes present (unstructured and structured littoral were sampled with seining nets and electrofishing; lentic were sampled with gillnets and longlines (deeper areas; and rapid water areas were sampled with cast nets. The ichthyofauna of the Canal da Piracema followed the pattern for South America and the Paraná River, with a predominance of Characiformes and Siluriformes. The most representative families were Characidae, Anostomidae, Pimelodidae and Loricariidae. We captured 116 species (17 were long-distance migratory during the period studied. Small-sized species were predominant in unstructured and structured littoral areas, especially Bryconamericus exodon and Apareiodon affinis.The most abundant species was Hypostomus spp. in lentic areas, followed by Iheringichthys labrosus. Hoplias aff. malabaricus predominated in deeper lentic areas. Long-distance migratory species were abundant in rapid waters; they were Prochilodus lineatus and Leporinus elongatus. The sharp reduction in the number of species, including migratory ones, is an indication that the Canal da Piracema is selecting the species that ascend it. Therefore, the search for information on the efficiency of the various fish passes present in the Canal da Piracema is fundamental, to facilitate upward movements of fish. If this is reached, this

  13. RFID Label Tag Design for Metallic Surface Environments

    Directory of Open Access Journals (Sweden)

    Ki Hwan Eom

    2011-01-01

    Full Text Available This paper describes a metal mount RFID tag that works reliably on metallic surfaces. The method proposes the use of commercial label type RFID tags with 2.5 mm thick Styrofoam103.7 with a relative permittivity of 1.03 attached on the back of the tag. In order to verify the performance of the proposed method, we performed experiments on an electric transformer supply chain system. The experimental results showed that the proposed tags can communicate with readers from a distance of 2 m. The recognition rates are comparable to those of commercial metallic mountable tags.

  14. Polarized tagged photons

    International Nuclear Information System (INIS)

    Maximon, L.C.; Ganz, Eric; Aniel, Thierry; Miniac, Arlette de.

    1982-03-01

    We consider in detail the differential cross section for polarized bremsstrahlung for angles and energies in the range of interest for a tagging system and derive a high energy, small angle approximation for this cross section. We use these approximations to determine the maxima and minima of the cross sections for these two polarization states, dσperpendicular and dσparallel, and to evaluate these cross sections at the extrema. It is shown that both dσperpendicular and dσparallel have a very sharp dip in the region of small momentum transfers. However, their behavior in the region of the dip, as a function of the azimuthal angle phi, is quite different over most of the photon spectrum. The cross section dσperpendicular behaves similarly to the cross section for unpolarized photons in that as phi increases, the sharp dip vanishes, the minimum fuses with the second maximum, and the cross section then has only a single maximum. In contrast, the sharp dip in the cross section dσparallel remains as phi increases. Coulomb corrections to the Born approximation are considered, and do not fill in these dips

  15. Mechanisms driving recruitment variability in fish: comparisons between the Laurentian Great Lakes and marine systems

    Science.gov (United States)

    Pritt, Jeremy J.; Roseman, Edward F.; O'Brien, Timothy P.

    2014-01-01

    In his seminal work, Hjort (in Fluctuations in the great fisheries of Northern Europe. Conseil Parmanent International Pour L'Exploration De La Mar. Rapports et Proces-Verbaux, 20: 1–228, 1914) observed that fish population levels fluctuated widely, year-class strength was set early in life, and egg production by adults could not alone explain variability in year-class strength. These observations laid the foundation for hypotheses on mechanisms driving recruitment variability in marine systems. More recently, researchers have sought to explain year-class strength of important fish in the Laurentian Great Lakes and some of the hypotheses developed for marine fisheries have been transferred to Great Lakes fish. We conducted a literature review to determine the applicability of marine recruitment hypotheses to Great Lakes fish. We found that temperature, interspecific interactions, and spawner effects (abundance, age, and condition of adults) were the most important factors in explaining recruitment variability in Great Lakes fish, whereas relatively fewer studies identified bottom-up trophodynamic factors or hydrodynamic factors as important. Next, we compared recruitment between Great Lakes and Baltic Sea fish populations and found no statistical difference in factors driving recruitment between the two systems, indicating that recruitment hypotheses may often be transferable between Great Lakes and marine systems. Many recruitment hypotheses developed for marine fish have yet to be applied to Great Lakes fish. We suggest that future research on recruitment in the Great Lakes should focus on forecasting the effects of climate change and invasive species. Further, because the Great Lakes are smaller and more enclosed than marine systems, and have abundant fishery-independent data, they are excellent candidates for future hypothesis testing on recruitment in fish.

  16. Do intracoelomic telemetry transmitters alter the post-release behaviour of migratory fish?

    Science.gov (United States)

    Wilson, Alexander D.M.; Hayden, Todd A.; Vandergoot, Christopher S.; Kraus, Richard T.; Dettmers, John M.; Cooke, Steven J.; Charles C. Krueger,

    2016-01-01

    Electronic tags have become a common tool in fish research, enhancing our understanding of how fish interact with their environment and move among different habitats, for estimating mortality and recording internal physiological states. An often-untested assumption of electronic tagging studies is that tagged fish are representative of untagged conspecifics and thus show ‘normal’ behaviour (e.g. movement rates, swimming activity, feeding). Here, we use a unique data set for potamadromous walleye (Sander vitreus) in Lake Huron and Lake Erie tributaries to assess whether the lack of appropriate controls in electronic tagging could seriously affect behavioural data. We used fish tagged in previous years and compared their migratory behaviour during the spawning season to fish tagged in a current year at the same location. The objective of the study was to determine whether intracoelomic acoustic tag implantation altered downstream movement of walleye after spawning. Fish tagged in a given season travelled slower downstream from two river spawning sites than fish tagged in previous years. Fish tagged one or two years earlier showed no differences between each other in downstream travel time, in contrast to fish tagged in a given year. Our results support notions that standard collection and intracoelomic tagging procedures can alter short-term behaviour (i.e. days, weeks, months), and as such, researchers should use caution when interpreting data collected over such time periods. Further, whenever possible, researchers should also explicitly evaluate post-tagging effects on behaviour as part of their experimental objectives.

  17. Long-term GPS tracking of ocean sunfish Mola mola offers a new direction in fish monitoring.

    Science.gov (United States)

    Sims, David W; Queiroz, Nuno; Humphries, Nicolas E; Lima, Fernando P; Hays, Graeme C

    2009-10-09

    Satellite tracking of large pelagic fish provides insights on free-ranging behaviour, distributions and population structuring. Up to now, such fish have been tracked remotely using two principal methods: direct positioning of transmitters by Argos polar-orbiting satellites, and satellite relay of tag-derived light-level data for post hoc track reconstruction. Error fields associated with positions determined by these methods range from hundreds of metres to hundreds of kilometres. However, low spatial accuracy of tracks masks important details, such as foraging patterns. Here we use a fast-acquisition global positioning system (Fastloc GPS) tag with remote data retrieval to track long-term movements, in near real time and position accuracy of GPS tagging to provide tracks of unparalleled accuracy for monitoring movements of large pelagic fish, and with nearly four times as many locations obtained by the GPS tag than by a conventional Argos transmitter. The results signal the potential of GPS-tagged pelagic fish that surface regularly to be detectors of resource 'hotspots' in the blue ocean and provides a new capability for understanding large pelagic fish behaviour and habitat use that is relevant to ocean management and species conservation.

  18. A high-speed, reconfigurable, channel- and time-tagged photon arrival recording system for intensity-interferometry and quantum optics experiments

    Science.gov (United States)

    Girish, B. S.; Pandey, Deepak; Ramachandran, Hema

    2017-08-01

    We present a compact, inexpensive multichannel module, APODAS (Avalanche Photodiode Output Data Acquisition System), capable of detecting 0.8 billion photons per second and providing real-time recording on a computer hard-disk, of channel- and time-tagged information of the arrival of upto 0.4 billion photons per second. Built around a Virtex-5 Field Programmable Gate Array (FPGA) unit, APODAS offers a temporal resolution of 5 nanoseconds with zero deadtime in data acquisition, utilising an efficient scheme for time and channel tagging and employing Gigabit ethernet for the transfer of data. Analysis tools have been developed on a Linux platform for multi-fold coincidence studies and time-delayed intensity interferometry. As illustrative examples, the second-order intensity correlation function ( g 2) of light from two commonly used sources in quantum optics —a coherent laser source and a dilute atomic vapour emitting spontaneously, constituting a thermal source— are presented. With easy reconfigurability and with no restriction on the total record length, APODAS can be readily used for studies over various time scales. This is demonstrated by using APODAS to reveal Rabi oscillations on nanosecond time scales in the emission of ultracold atoms, on the one hand, and, on the other hand, to measure the second-order correlation function on the millisecond time scales from tailored light sources. The efficient and versatile performance of APODAS promises its utility in diverse fields, like quantum optics, quantum communication, nuclear physics, astrophysics and biology.

  19. Columbia River System Operation Review final environmental impact statement. Appendix K: Resident fish

    International Nuclear Information System (INIS)

    1995-11-01

    The System Operation Review (SOR) is a study and environmental compliance process being used by the three Federal agencies to analyze future operations of the system and river use issues. The goal of the SOR is to achieve a coordinated system operation strategy for the river that better meets the needs of all river users. This technical appendix addresses only the effects of alternative system operating strategies for managing the Columbia River system. In this appendix the Resident Fish Work Group (RFWG) has attempted to characterize and evaluate impacts of dam operation on an extremely complex and diverse integrated resource. Not only is this required under the National Environmental Policy Act (NEPA) for SOR, there are resident fish populations that have status under the Federal Endangered Species Act (ESA) or equivalent state regulations (Kootenai River white sturgeon, Snake River white sturgeon, sandroller, shorthead and torrent sculpins, bull trout, westslope cutthroat trout, redband trout, and burbot). The RFWG has also attempted to develop operating alternatives that benefit not only resident fish, but anadromous fish, wildlife, and other human interests as well. The authors have recognized the co-evolution of resident fish, anadromous fish, and other integrated resources in the basin

  20. Development of a Natural Rearing System to Improve Supplemental Fish Quality, 1991-1995 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Maynard, Desmond J.; Flagg, Thomas A.; Mahnken, Conrad V.W.

    1996-08-01

    In this report, the National Marine Fisheries Service (NMFS), in collaboration with the Bonneville Power Administration (BPA), the Washington State Department of Fish and Wildlife (WDFW), and the US Fish and Wildlife Service (USFWS), presents research findings and guidelines for development and evaluation of innovative culture techniques to increase postrelease survival of hatchery fish. The Natural Rearing Enhancement System (NATURES) described in this report is a collection of experimental approaches designed to produce hatchery-reared chinook salmon (Oncorhynchus tshawytscha) that exhibit wild-like behavior, physiology, and morphology. The NATURES culture research for salmonids included multiple tests to develop techniques such as: raceways equipped with cover, structure, and natural substrates to promote development of proper body camouflage coloration; feed-delivery systems that condition fish to orient to the bottom rather than the surface of the rearing vessel; predator conditioning of fish to train them to avoid predators; and supplementing diets with natural live foods to improve foraging ability. The underlying assumptions are that NATURES will: (1) promote the development of natural cryptic coloration and antipredator behavior; (2) increase postrelease foraging efficiency; (3) improve fish health and condition by alleviating chronic, artificial rearing habitat-induced stress; and (4) reduce potential genetic selection pressures induced by the conventional salmon culture environment. A goal in using NATURES is to provide quality fish for rebuilding depleted natural runs.

  1. Tag-to-Tag Interference Suppression Technique Based on Time Division for RFID

    Directory of Open Access Journals (Sweden)

    Grishma Khadka

    2017-01-01

    Full Text Available Radio-frequency identification (RFID is a tracking technology that enables immediate automatic object identification and rapid data sharing for a wide variety of modern applications using radio waves for data transmission from a tag to a reader. RFID is already well established in technical areas, and many companies have developed corresponding standards and measurement techniques. In the construction industry, effective monitoring of materials and equipment is an important task, and RFID helps to improve monitoring and controlling capabilities, in addition to enabling automation for construction projects. However, on construction sites, there are many tagged objects and multiple RFID tags that may interfere with each other’s communications. This reduces the reliability and efficiency of the RFID system. In this paper, we propose an anti-collision algorithm for communication between multiple tags and a reader. In order to suppress interference signals from multiple neighboring tags, the proposed algorithm employs the time-division (TD technique, where tags in the interrogation zone are assigned a specific time slot so that at every instance in time, a reader communicates with tags using the specific time slot. We present representative computer simulation examples to illustrate the performance of the proposed anti-collision technique for multiple RFID tags.

  2. 77 FR 76367 - Availability of E-Tag Information to Commission Staff

    Science.gov (United States)

    2012-12-28

    ...-Tag Access to MMUs, RTOs and ISOs........ 43 1. E-Tag NOPR 43 2. Comments 44 3. Commission... requires that Regional Transmission Organizations (RTOs), Independent System Operators (ISOs) and their... MMUs for RTOs and ISOs to have access to complete e-Tag information, including access to e-Tags for...

  3. Optimization of Fish Protection System to Increase Technosphere Safety

    Science.gov (United States)

    Khetsuriani, E. D.; Fesenko, L. N.; Larin, D. S.

    2017-11-01

    The article is concerned with field study data. Drawing upon prior information and considering structural features of fish protection devices, we decided to conduct experimental research while changing three parameters: process pressure PCT, stream velocity Vp and washer nozzle inclination angle αc. The variability intervals of examined factors are shown in the Table 1. The conicity angle was assumed as a constant one. The box design B3 was chosen as a baseline being close to D-optimal designs in its statistical characteristics. The number of device rotations and its fish fry protection efficiency were accepted as the output functions of optimization. The numerical values of regression coefficients of quadratic equations describing the behavior of optimization functions Y1 and Y2 and their formulaic errors were calculated upon the test results in accordance with the planning matrix. The adequacy or inadequacy of the obtained quadratic regression model is judged via checking the condition whether Fexp ≤ Ftheor.

  4. Melanocortin systems on pigment dispersion in fish chromatophores.

    Science.gov (United States)

    Kobayashi, Yuki; Mizusawa, Kanta; Saito, Yumiko; Takahashi, Akiyoshi

    2012-01-01

    α-Melanocyte-stimulating hormone (α-MSH) is responsible for pigment dispersion in the chromatophores of fish and other tetrapods such as amphibians and reptiles. Recently, we discovered that α-MSH did not always stimulate pigment dispersion because this hormonal peptide exerted no effects on the melanophores of flounders. We assumed that the reduction of α-MSH activity was related to the co-expression of different α-MSH receptor subtypes - termed melanocortin receptors (MCR) - a member of G-protein-coupled receptors (GPCR) - based on several reports demonstrating that GPCR forms heterodimers with various properties that are distinct from those of the corresponding monomers. In this review, we summarize the relationships between the pigment-dispersing activity of α-MSH-related peptides, molecular forms of α-MSH-related peptides, and mcr subtypes expressed in fish chromatophores.

  5. Melanocortin systems on pigment dispersion in fish chromatophores

    Directory of Open Access Journals (Sweden)

    Yuki eKobayashi

    2012-02-01

    Full Text Available Alpha-Melanocyte-stimulating hormone (alpha-MSH is responsible for pigment dispersion in the chromatophores of fish and other tetrapods such as amphibians and reptiles. Recently, we discovered that alpha-MSH did not always stimulate pigment dispersion because this hormonal peptide exerted no effects on the melanophores of flounders. We assumed that the reduction of alpha-MSH activity was related to the co-expression of different alpha-MSH receptor subtypes—termed melanocortin receptors (MCR—a member of G-protein-coupled receptors (GPCR—based on several reports demonstrating that GPCR forms heterodimers with various properties that are distinct from those of the corresponding monomers. In this review, we summarize the relationships between the pigment-dispersing activity of alpha-MSH-related peptides, molecular forms of alpha-MSH-related peptides, and Mcr subtypes expressed in fish chromatophores.

  6. User Interface Program for secure electronic tags

    International Nuclear Information System (INIS)

    Cai, Y.; Koehl, E.R.; Carlson, R.D.; Raptis, A.C.

    1995-05-01

    This report summarizes and documents the efforts of Argonne National Laboratory (ANL) in developing a secure tag communication user interface program comprising a tag monitor and a communication tool. This program can perform the same functions as the software that was developed at the Lawrence Livermore National Laboratory (LLNL), but it is enhanced with a user-friendly screen. It represents the first step in updating the TRANSCOM Tracking System (TRANSCOM) by incorporating a tag communication screen menu into the main menu of the TRANSCOM user program. A working version of TRANSCOM, enhanced with ANL secure-tag graphics, will strongly support the Department of Energy Warhead Dismantlement/Special Nuclear Materials Control initiatives. It will allow commercial satellite tracking of the movements and operational activities of treaty-limited items and transportation vehicles throughout Europe and the former USSR, as well as the continental US

  7. Inclusive Flavour Tagging Algorithm

    International Nuclear Information System (INIS)

    Likhomanenko, Tatiana; Derkach, Denis; Rogozhnikov, Alex

    2016-01-01

    Identifying the flavour of neutral B mesons production is one of the most important components needed in the study of time-dependent CP violation. The harsh environment of the Large Hadron Collider makes it particularly hard to succeed in this task. We present an inclusive flavour-tagging algorithm as an upgrade of the algorithms currently used by the LHCb experiment. Specifically, a probabilistic model which efficiently combines information from reconstructed vertices and tracks using machine learning is proposed. The algorithm does not use information about underlying physics process. It reduces the dependence on the performance of lower level identification capacities and thus increases the overall performance. The proposed inclusive flavour-tagging algorithm is applicable to tag the flavour of B mesons in any proton-proton experiment. (paper)

  8. Ecological mechanisms underlying the sustainability of the agricultural heritage rice-fish coculture system.

    Science.gov (United States)

    Xie, Jian; Hu, Liangliang; Tang, Jianjun; Wu, Xue; Li, Nana; Yuan, Yongge; Yang, Haishui; Zhang, Jiaen; Luo, Shiming; Chen, Xin

    2011-12-13

    For centuries, traditional agricultural systems have contributed to food and livelihood security throughout the world. Recognizing the ecological legacy in the traditional agricultural systems may help us develop novel sustainable agriculture. We examine how rice-fish coculture (RF), which has been designated a "globally important agricultural heritage system," has been maintained for over 1,200 y in south China. A field survey demonstrated that although rice yield and rice-yield stability are similar in RF and rice monoculture (RM), RF requires 68% less pesticide and 24% less chemical fertilizer than RM. A field experiment confirmed this result. We documented that a mutually beneficial relationship between rice and fish develops in RF: Fish reduce rice pests and rice favors fish by moderating the water environment. This positive relationship between rice and fish reduces the need for pesticides in RF. Our results also indicate a complementary use of nitrogen (N) between rice and fish in RF, resulting in low N fertilizer application and low N release into the environment. These findings provide unique insights into how positive interactions and complementary use of resource between species generate emergent ecosystem properties and how modern agricultural systems might be improved by exploiting synergies between species.

  9. Fish habitat preferences in an artificial reservoir system

    Czech Academy of Sciences Publication Activity Database

    Prchalová, Marie; Kubečka, Jan; Hladík, Milan; Hohausová, Eva; Čech, Martin; Frouzová, Jaroslava

    2006-01-01

    Roč. 29, č. 4 (2006), s. 1890-1894 ISSN 0368-0770. [Congress of SIL - International association of theoretical and applied limnology /29./. Lahti, 08.08.2004-14.08.2004] R&D Projects: GA ČR(CZ) GA206/02/0520 Institutional research plan: CEZ:AV0Z60170517 Keywords : multivariate analysis * fish community * reservoirs * spatial distribution Subject RIV: EH - Ecology, Behaviour

  10. Structural and functional effects of heavy metals on the nervous system, including sense organs, of fish

    DEFF Research Database (Denmark)

    Baatrup, E

    1991-01-01

    metals are well known pollutants in the aquatic environment. Their interaction with relevant chemical stimuli may interfere with the communication between fish and environment. 5. The affinity for a number of ligands and macromolecules makes heavy metals most potent neurotoxins. 6. The present Mini......1. Today, fish in the environment are inevitably exposed to chemical pollution. Although most hazardous substances are present at concentrations far below the lethal level, they may still cause serious damage to the life processes of these animals. 2. Fish depend on an intact nervous system......, including their sense organs, for mediating relevant behaviour such as food search, predator recognition, communication and orientation. 3. Unfortunately, the nervous system is most vulnerable and injuries to its elements may dramatically change the behaviour and consequently the survival of fish. 4. Heavy...

  11. Implications of using alternative methods of vessel monitoring system (VMS) data analysis to describe fishing activities and impacts

    NARCIS (Netherlands)

    Lambert, G.I.; Jennings, S.; Hiddink, J.G.; Hintzen, N.T.; Hinz, H.; Kaiser, M.J.; Murray, L.G.

    2012-01-01

    Understanding the spatial distribution and intensity of fishing activity is a prerequisite for estimating fishing impacts on seabed biota and habitats. Vessel monitoring system data provide information on fishing activity at large spatial scales. However, successive position records can be too

  12. Product-service system method to measure sustainability level of traditional smoked fish processing industries

    OpenAIRE

    Purwaningsih Ratna; Cahyantari Anggaina Elfandora; Ariyani Zulfaida; Susanty Aries; Arvianto Ary; Santoso Haryo

    2018-01-01

    Small Medium Enterprise’s (SME) of traditional fish processing at Semarang, Central Java, Indonesia still focus their business on gain more profits. Sustainability aspect has not received enough attention yet. This study aims to review the sustainability level of SME smoked fish Semarang using product service system (PSS) method. PSS consists of three dimensions (1) Environment, (2) Socio-cultural and (3) Economic. Each dimension consists of 6 criteria's. PSS not only assess the level of sust...

  13. Growth of plume ''resident'' fishes in Lake Michigan

    International Nuclear Information System (INIS)

    Spigarelli, S.A.; Smith, D.W.

    1974-01-01

    Brown trout, rainbow trout, and chinook salmon were collected from the Point Beach thermal discharge area, tagged with commercial dart tags and temperature-sensitive tags, and released back into the discharge area. RNA and DNA analyses were performed on epaxial muscle samples taken from each tagged fish recaptured in the plume area and from control fish. A table is presented to show mean weights, condition factors, and RNA-DNA ratios for each group of fish. Results indicated that the fish did not experience any severe growth abnormalities as a result of their residence in the thermal plume area

  14. Extensive review of fish embryo acute toxicities for the prediction of GHS acute systemic toxicity categories.

    Science.gov (United States)

    Scholz, Stefan; Ortmann, Julia; Klüver, Nils; Léonard, Marc

    2014-08-01

    Distribution and marketing of chemicals require appropriate labelling of health, physical and environmental hazards according to the United Nations global harmonisation system (GHS). Labelling for (human) acute toxicity categories is based on experimental findings usually obtained by oral, dermal or inhalative exposure of rodents. There is a strong societal demand for replacing animal experiments conducted for safety assessment of chemicals. Fish embryos are considered as alternative to animal testing and are proposed as predictive model both for environmental and human health effects. Therefore, we tested whether LC50s of the fish embryo acute toxicity test would allow effectively predicting of acute mammalian toxicity categories. A database of published fish embryo LC50 containing 641 compounds was established. For these compounds corresponding rat oral LD50 were identified resulting in 364 compounds for which both fish embryo LC50 and rat LD50 was available. Only a weak correlation of fish embryo LC50 and rat oral LD50 was obtained. Fish embryos were also not able to effectively predict GHS oral acute toxicity categories. We concluded that due to fundamental exposure protocol differences (single oral dose versus water-borne exposure) a reverse dosimetry approach is needed to explore the predictive capacity of fish embryos. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. Contribution of galloylation and polymerization to the antioxidant activity of polyphenols in fish lipid systems.

    Science.gov (United States)

    Iglesias, Jacobo; Pazos, Manuel; Lois, Salomé; Medina, Isabel

    2010-06-23

    Polyphenolic fractions extracted from pine (Pinus pinaster) bark, grape (Vitis vinifera) pomace, and witch hazel (Hamamelis virginiana) bark were selected for investigating the influence of the number of phenolic units, polymerization, and the content of esterified galloyl residues (galloylation) on their efficacy for inhibiting lipid oxidation in fish lipid enriched foodstuffs. Experiments carried out with nongalloylated pine bark fractions with different polymerization degrees demonstrated that the number of catechin residues per molecule modulates their reducing and chelating properties in solution. In real food systems such as bulk fish oil and fish oil-in-water emulsions, the efficacy against lipid oxidation was highly dependent on the physical location of the antioxidant at the oxidative sensitive sites. The lowest polymerized fractions were the most efficient in bulk fish oil samples, whereas proanthocyanidins with an intermediate polymerization degree showed the highest activity in fish oil-in-water emulsions. Galloylation did not influence the antioxidant effectiveness of proanthocyanidins in bulk fish oils. The presence of galloyl groups favored the antioxidant activity of the polyphenols in emulsions, although results indicated that a high degree of galloylation did not improve significantly the activity found with medium galloylated proanthocyanidins. The results obtained in this research provide useful information about the relationship between structure and antioxidant activity in order to design antioxidant additives with application in fish oil-enriched functional foods.

  16. FBIS: A regional DNA barcode archival & analysis system for Indian fishes

    Science.gov (United States)

    Nagpure, Naresh Sahebrao; Rashid, Iliyas; Pathak, Ajey Kumar; Singh, Mahender; Singh, Shri Prakash; Sarkar, Uttam Kumar

    2012-01-01

    DNA barcode is a new tool for taxon recognition and classification of biological organisms based on sequence of a fragment of mitochondrial gene, cytochrome c oxidase I (COI). In view of the growing importance of the fish DNA barcoding for species identification, molecular taxonomy and fish diversity conservation, we developed a Fish Barcode Information System (FBIS) for Indian fishes, which will serve as a regional DNA barcode archival and analysis system. The database presently contains 2334 sequence records of COI gene for 472 aquatic species belonging to 39 orders and 136 families, collected from available published data sources. Additionally, it contains information on phenotype, distribution and IUCN Red List status of fishes. The web version of FBIS was designed using MySQL, Perl and PHP under Linux operating platform to (a) store and manage the acquisition (b) analyze and explore DNA barcode records (c) identify species and estimate genetic divergence. FBIS has also been integrated with appropriate tools for retrieving and viewing information about the database statistics and taxonomy. It is expected that FBIS would be useful as a potent information system in fish molecular taxonomy, phylogeny and genomics. Availability The database is available for free at http://mail.nbfgr.res.in/fbis/ PMID:22715304

  17. Sperm motility in fish: technical applications and perspectives through CASA-Mot systems.

    Science.gov (United States)

    Gallego, V; Asturiano, J F

    2018-03-09

    Although a relatively high number of sperm quality biomarkers have been reported over the years in several fish species, sperm motility is nowadays considered the best biomarker for fish spermatozoa. The first scientific reports focusing on fish sperm motility date from a century ago, but the objective assessment allowed by computer-aided sperm analysis (CASA-Mot) systems was not applied to fish species until the mid-1980s. Since then, a high number of sperm kinetic parameters from more than 170 fish species have been reported in more than 700 scientific articles, covering a wide range of topics, such as sperm physiology, sperm storage, broodstock management, the phenomenon of sperm competition, ecotoxicology and understanding the life cycle of the species. The sperm kinetic parameters provided by CASA-Mot systems can serve as powerful and useful tools for aquaculture and ecological purposes, and this review provides an overview of the major research areas in which fish sperm motility assessment by a CASA-Mot system has been used successfully.

  18. Sensitivity of marine systems to climate and fishing: Concepts, issues and management responses

    DEFF Research Database (Denmark)

    Perry, Ian; Cury, Philippe; Brander, Keith

    2010-01-01

    forcing. Fishing is unlikely to alter the sensitivities of individual finfish and invertebrates to climate forcing. It will remove individuals with specific characteristics from the gene pool, thereby affecting structure and function at higher levels of organisation. Fishing leads to a loss of older age......Modern fisheries research and management must understand and take account of the interactions between climate and fishing, rather than try to disentangle their effects and address each separately. These interactions are significant drivers of change in exploited marine systems and have...... but will be manifest as the accumulation of the interactions between fishing and climate variability — unless threshold limits are exceeded. Marine resource managers need to develop approaches which maintain the resilience of individuals, populations, communities and ecosystems to the combined and interacting effects...

  19. Use Carum copticum essential oil for controlling the Listeria monocytogenes growth in fish model system

    Directory of Open Access Journals (Sweden)

    Soghra Rabiey

    2014-01-01

    Full Text Available This study was conducted to evaluate the antibacterial effect of Carum copticum essential oil (Ajowan EO against Listeria monocytogenes in fish model system. Ajowan EO chemical composition was determined by gas chromatography/mass spectral analysis and the highest concentration of Carum copticum essential oil without any significant changes on sensory properties of kutum fish (Rutilus frisii kutum was assigned. Then the inhibitory effect of Ajowan EO at different concentrations in presence of salt and smoke component was tested on L. monocytogenes growth in fish peptone broth (FPB, kutum broth and cold smoked kutum broth at 4 ºC for 12 days. Ajowan EO completely decreased the number of L. monocytogenes in FPB after 12 days of storage, however, antimicrobial effect of EO significantly reduced in kutum and cold smoked kutum broth. Addition of 4% NaCl and smoke component improved the anti-listerial activity of Ajowan EO in all fish model broths.

  20. Development of a Natural Rearing System to Improve Supplemental Fish Quality, 1999-2003 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Maynard, Desmond J.

    2003-02-25

    The National Marine Fisheries Service (NMFS) has been conducting Natural Rearing Enhancement System (NATURES) research since the early 1990s. NATURES studies have looked at a variety of mechanisms to enhance production of wild-like salmonids from hatcheries. The goal of NATURES research is to develop fish culture techniques that enable hatcheries to produce salmon with more wild-like characteristics and increased postrelease survival. The development of such techniques is called for in the Columbia Basin Fish and Wildlife Program. This document is the draft report for the Supplemental Fish Quality Contract DE-AI79-91BP20651 Over the history of the project, the effects of seminatural raceway habitats, automated underwater feeders, exercise current velocities, live food diets, and predator avoidance training have been investigated. The findings of these studies are reported in an earlier contract report (Maynard et al. 1996a). The current report focuses on research that has been conducted between 1999 and 2002. This includes studies on the effect of exercise on salmon and steelhead trout, effects of predator avoid training, integration of NATUES protocols into production hatcheries, and the study of social behavior of steelhead grown in enriched and conventional environments. Traditionally, salmon (Oncorhynchus spp.) are reared in barren concrete raceways that lack natural substrate, in-stream structure, or overhead cover. The fish are fed in an unnatural manner with artificial feeds mechanically or hand broadcast across the water surface. This traditional approach has increased the egg-to-smolt survival of hatchery-reared fish by an order of magnitude over that experienced by wild-reared salmon. However, once hatchery-reared fish are released into the wild their smolt-to-adult survival is usually much lower than wild-reared salmon. The reduced postrelease survival of hatchery-reared fish may stem from differences in their behavior and morphology compared to wild

  1. Tagging: An Organization Scheme for the Internet

    Directory of Open Access Journals (Sweden)

    Marijke A. Visser

    2010-03-01

    Full Text Available How should the information on the Internet be organized? This question and the possible solutions spark debates among people concerned with how we identify, classify, and retrieve Internet content. This paper discusses the benefits and the controversies of using a tagging system to organize Internet resources. Tagging refers to a classification system where individual Internet users apply labels, or tags, to digital resources. Tagging increased in popularity with the advent of Web 2.0 applications that encourage interaction among users. As more information is available digitally, the challenge to find an organizational system scalable to the Internet will continue to require forward thinking. Trained to ensure access to a range of informational resources, librarians need to be concerned with access to Internet content. Librarians can play a pivotal role by advocating for a system that supports the user at the moment of need. Tagging may just be the necessary system.

  2. Factors Affecting Detection Probability of Acoustic Tags in Coral Reefs

    KAUST Repository

    Bermudez, Edgar F.

    2012-05-01

    Acoustic telemetry is an important tool for studying the movement patterns, behaviour, and site fidelity of marine organisms; however, its application is challenged in coral reef environments where complex topography and intense environmental noise interferes with acoustic signals, and there has been less study. Therefore, it is particularly critical in coral reef telemetry studies to first conduct a long-term range test, a tool that provides informa- tion on the variability and periodicity of the transmitter detection range and the detection probability. A one-month range test of a coded telemetric system was conducted prior to a large-scale tagging project investigating the movement of approximately 400 fishes from 30 species on offshore coral reefs in the central Red Sea. During this range test we determined the effect of the following factors on transmitter detection efficiency: distance from receiver, time of day, depth, wind, current, moon-phase and temperature. The experiment showed that biological noise is likely to be responsible for a diel pattern of -on average- twice as many detections during the day as during the night. Biological noise appears to be the most important noise source in coral reefs overwhelming the effect of wind-driven noise, which is important in other studies. Detection probability is also heavily influenced by the location of the acoustic sensor within the reef structure. Understanding the effect of environmental factors on transmitter detection probability allowed us to design a more effective receiver array for the large-scale tagging study.

  3. AFSC/RACE/GAP/Nichol: Archival tag depth and temperature data from northern rock sole

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Depth data from archival tags on northern rock sole (Lepidopsetta polyxystra) were examined to assess whether fish used tidal currents to aid horizontal migration....

  4. The design and analysis of salmonid tagging studies in the Columbia Basin. Volume 2: Estimating salmonid survival with combined PIT-CWT tagging. Technical report

    International Nuclear Information System (INIS)

    Newman, K.

    1997-06-01

    Passive Integrated Transponder (PIT) tags and Coded Wire Tags (CWTs) in combination can provide information about salmonid survival that single tag releases may not. The release and recapture protocol affects which survival and recapture rates can be estimated and the precision of the estimates. For the particular case of Columbia river salmonids tagged with both PIT tags and CWTs, three different release and recapture protocols were evaluated. This report addresses the need to study the fate of salmon smolt in-river and their subsequent return as adults. Double-tagging procedures are investigated where PIT-tags would be used to provide in-river survival data during smolt outmigrations and coded-wire tags (CWT) used to provide adult return information. This report provides statistical models for the analysis of the joint data as well as recommendations on optimal tagging studies. Study costs and stress on smolt can be reduced by only PIT-tagging a subset of all the fish coded-wire-tagged, while retaining the information content and sampling precision

  5. Mining of biomarker genes from expressed sequence tags and differential display reverse transcriptase-polymerase chain reaction in the self-fertilizing fish, Kryptolebias marmoratus and their expression patterns in response to exposure to an endocrine-disrupting alkylphenol, bisphenol A.

    Science.gov (United States)

    Lee, Young-Mi; Rhee, Jae-Sung; Hwang, Dae-Sik; Kim, Il-Chan; Raisuddin, Sheikh; Lee, Jae-Seong

    2007-06-30

    Expressed sequence tags (ESTs) and differentially expressed cDNAs from the self-fertilizing fish, Kryptolebias marmoratus were mined to develop alternative biomarkers for endocrine-disrupting chemicals (EDCs). 1,577 K. marmoratus cDNA clones were randomly sequenced from the 5'-end. These clones corresponded to 1,518 and 1,519 genes in medaka dbEST and zebrafish dbEST, respectively. Of the matched genes, 197 and 115 genes obtained Unigene IDs in medaka dbEST and zebrafish dbEST, respectively. Many of the annotated genes are potential biomarkers for environmental stresses. In a differential display reverse transcriptase-polymerase chain reaction (DD RT-PCR) study, 56 differential expressed genes were obtained from fish liver exposed to bisphenol A. Of these, 16 genes were identified after BLAST search to GenBank, and the annotated genes were mainly involved in catalytic activity and binding. The expression patterns of these 16 genes were validated by real-time RT-PCR of liver tissue from fish exposed to bisphenol A. Our findings suggest that expression of these 16 genes is modulated by endocrine disrupting chemicals, and therefore that they are potential biomarkers for environmental stress including EDCs exposure.

  6. Tag cloud generation for results of multiple keywords queries

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2013-01-01

    In this paper we study tag cloud generation for retrieved results of multiple keyword queries. It is motivated by many real world scenarios such as personalization tasks, surveillance systems and information retrieval tasks defined with multiple keywords. We adjust the state-of-the-art tag cloud...... generation techniques for multiple keywords query results. Consequently, we conduct the extensive evaluation on top of three distinct collaborative tagging systems. The graph-based methods perform significantly better for the Movielens and Bibsonomy datasets. Tag cloud generation based on maximal coverage...

  7. Population Aspects of Fishes in Geba and Sor Rivers, White Nile System in Ethiopia, East Africa

    Directory of Open Access Journals (Sweden)

    Simagegnew Melaku

    2017-01-01

    Full Text Available This study was carried out to assess the diversity, condition factor, length-weight relationship, and sex ratio of fishes in Geba and Sor Rivers located in Baro-Akobo Basin, White Nile system within Ethiopia. Fish samples were collected in one wet and one dry season. The length-weight relationships were fitted using power equation for the most abundant species. A total of 348 fish specimens were collected using gillnets and hooks. These were identified into eight species and one Garra sp. representing seven genera and four families. Family Cyprinidae was the most dominant with six species (66.7%. Labeobarbus intermedius, Labeobarbus nedgia, and Labeo cylindricus were the most abundant fish species, respectively, with 60.72%, 16.83%, and 14.66% index of relative importance (IRI. The diversity index was higher for Geba River (H′ = 1.50 than for Sor River (H′ = 1.10. All the three most abundant species had negative allometric growth. Seasonal variations in the mean Fulton condition factor (FCF were statistically significant for L. cylindricus (p<0.05. There was variation in the sex ratio with the females dominating in all the three most abundant species. Further investigation into the fish diversity, food, feeding, and reproductive behaviors of fish species especially in the tributaries of these rivers and their socioeconomic aspects is recommended.

  8. Fish assemblage structure and habitat associations in a large western river system

    Science.gov (United States)

    Smith, C.D.; Quist, Michael C.; Hardy, R. S.

    2016-01-01

    Longitudinal gradients of fish assemblage and habitat structure were investigated in the Kootenai River of northern Idaho. A total of 43 500-m river reaches was sampled repeatedly with several techniques (boat-mounted electrofishing, hoop nets and benthic trawls) in the summers of 2012 and 2013. Differences in habitat and fish assemblage structure were apparent along the longitudinal gradient of the Kootenai River. Habitat characteristics (e.g. depth, substrate composition and water velocity) were related to fish assemblage structure in three different geomorphic river sections. Upper river sections were characterized by native salmonids (e.g. mountain whitefish Prosopium williamsoni), whereas native cyprinids (peamouth Mylocheilus caurinus, northern pikeminnow Ptychocheilus oregonensis) and non-native fishes (pumpkinseed Lepomis gibbosus, yellow perch Perca flavescens) were common in the downstream section. Overall, a general pattern of species addition from upstream to downstream sections was discovered and is likely related to increased habitat complexity and additions of non-native species in downstream sections. Assemblage structure of the upper sections were similar, but were both dissimilar to the lower section of the Kootenai River. Species-specific hurdle regressions indicated the relationships among habitat characteristics and the predicted probability of occurrence and relative abundance varied by species. Understanding fish assemblage structure in relation to habitat could improve conservation efforts of rare fishes and improve management of coldwater river systems.

  9. Aquatic systems in and adjacent to Agulhas National Park with particular reference to the fish fauna

    Directory of Open Access Journals (Sweden)

    I.A. Russell

    2006-12-01

    Full Text Available The study aimed to determine the distribution and relative abundance of freshwater fishes in and adjacent to Agulhas National Park. Fourteen fish species were collected during surveys carried out in the Heuningnes and Ratel river systems and a variety of endorheic pans from 2003 to 2005. Seven of the species are marine fishes tolerant of low salinity (Mugil cephalus, Liza richardsonii, Monodactylus falciformis, Caffrogobius gilchristi, Rhabdosargus holubi, Solea bleekeri, Gilchristella aestuaria with the remaining seven species being primary freshwater fishes. Three are indigenous, including Pseudobarbus burchelli, Sandelia capensis, and Galaxias zebratus of which two morphological forms were recorded within Agulhas National Park. The remaining four species are invasive aliens (Cyprinus carpio, Lepomis macrochirus, Micropterus salmoides, Micropterus punctulatus. Classification using physico-chemical variables indicated four major wetland groups, with fishes occurring predominantly in the group comprising palustrine, lacustrine and riverine wetlands, and in one instance in a brackish endorheic pan. Management actions which should facilitate conservation of indigenous fishes in the Agulhas region are suggested.

  10. Diagnostic model for assessing traceability system performance in fish processing plants

    NARCIS (Netherlands)

    Mgonja, J.T.; Luning, P.A.; Vorst, van der J.G.A.J.

    2013-01-01

    This paper introduces a diagnostic tool that can be used by fish processing companies to evaluate their own traceability systems in a systematic manner. The paper begins with discussions on the rationale of traceability systems in food manufacturing companies, followed by a detailed analysis of the

  11. New approach for monitoring fish stress: A novel enzyme-functionalized label-free immunosensor system for detecting cortisol levels in fish.

    Science.gov (United States)

    Wu, Haiyun; Ohnuki, Hitoshi; Ota, Shirei; Murata, Masataka; Yoshiura, Yasutoshi; Endo, Hideaki

    2017-07-15

    Fishes display a wide variation in their physiological responses to stress, which is clearly evident in the plasma corticosteroid changes, chiefly cortisol levels in fish. As a well-known indicator of fish stress, a simple and rapid method for detecting cortisol changes especially sudden increases is desired. In this study, we describe an enzyme-functionalized label-free immunosensor system for detecting fish cortisol levels. Detection of cortisol using amperometry was achieved by immobilizing both anti-cortisol antibody (selective detection of cortisol) and glucose oxidase (signal amplification and non-toxic measurement) on an Au electrode surface with a self-assembled monolayer. This system is based on the maximum glucose oxidation output current change induced by the generation of a non-conductive antigen-antibody complex, which depends on the levels of cortisol in the sample. The immunosensor responded to cortisol levels with a linear decrease in the current in the range of 1.25-200ngml -1 (R=0.964). Since the dynamic range of the sensor can cover the normal range of plasma cortisol in fish, the samples obtained from the fish did not need to be diluted. Further, electrochemical measurement of one sample required only ~30min. The sensor system was applied to determine the cortisol levels in plasma sampled from Nile tilapia (Oreochromis niloticus), which were then compared with levels of the same samples determined using the conventional method (ELISA). Values determined using both methods were well correlated. These findings suggest that the proposed label-free immunosensor could be useful for rapid and convenient analysis of cortisol levels in fish without sample dilution. We also believe that the proposed system could be integrated in a miniaturized potentiostat for point-of-care cortisol detection and useful as a portable diagnostic in fish farms in the future. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Preliminary evaluation of the effectiveness of visible implant elastomer and coded wire tags for tagging young-of-the-year Atlantic sturgeon, Acipenser oxyrinchus

    Directory of Open Access Journals (Sweden)

    Kapusta Andrzej

    2015-12-01

    Full Text Available The aim of the study was to determine the retention rates of visible implant elastomer (VIE and coded wire tags (CWT and the impact tagging had on the growth of Atlantic sturgeon, Acipenser oxyrinchus Mitchill, during an eight-week rearing period under laboratory conditions. Two size groups of young-of-the-year (YOY sturgeon were used in the study. The tagging was not found to have a significant impact on the final total length or body weight or the condition coefficient of the sturgeon from either size group. Sturgeon survival in the different groups ranged from 90.6 to 100%. Mortality was not noted until two (CWT and four (VIE weeks following tagging and was probably not linked to tagging. The retention rate for VIE tags implanted in the rostrum in both size groups was 100%, while for tags implanted at the base of the pectoral fin was 93.5%. The retention of CWT in the smaller fish was 90%, and in the larger sturgeon it was 100%. Tagging small sturgeon with CWT and VIE is minimally invasive, and it did not impact the growth or condition of the tagged fish.

  13. Application of the target fish community model to an urban river system.

    Science.gov (United States)

    Meixler, Marcia S

    2011-04-01

    Several models have been developed to assess the biological integrity of aquatic systems using fish community data. One of these, the target fish community (TFC) model, has been used primarily to assess the biological integrity of larger, mainstem rivers in southern New England with basins characterized by dispersed human activities. We tested the efficacy of the TFC approach to specify the fish community in the highly urbanized Charles River watershed in eastern Massachusetts. To create a TFC for the Charles River we assembled a list of fish species that historically inhabited the Charles River watershed, identified geomorphically and zoogeographically similar reference rivers regarded as being in high quality condition, amassed fish survey data for the reference rivers, and extracted from the collections the information needed to define a TFC. We used a similarity measurement method to assess the extent to which the study river community complies with the TFC and an inference approach to summarize the manner in which the existing fish community differed from target conditions. The five most abundant species in the TFC were common shiners (34%), fallfish (17%) redbreast sunfish (11%), white suckers (8%), and American eel (7%). Three of the five species predicted to be most abundant in the TFC were scarce or absent in the existing river community. Further, the river was dominated by macrohabitat generalists (99%) while the TFC was predicted to contain 19% fluvial specialist species, 43% fluvial dependent species, and 38% macrohabitat generalist species. In addition, while the target community was dominated by fish intolerant (37%) and moderately tolerant (39%) of water quality degradation, the existing community was dominated by tolerant individuals (59%) and lacked intolerant species expected in the TFC. Similarity scores for species, habitat use specialization, and water quality degradation tolerance categories were 28%, 35% and 66%, respectively. The clear

  14. Effects of fishing technique on assessing species composition in aquatic systems in semi-arid Brazil

    Directory of Open Access Journals (Sweden)

    ESF Medeiros

    Full Text Available In most ecological field research, appropriate sampling is critical for the understanding of processes underlying fish populations and communities, and is even more important in heterogeneous environments such as the aquatic systems of the semi-arid region of Brazil. This study intends to make a contribution to the development of sampling programs and gear selection in aquatic systems of semi-arid Brazil by evaluating the effects of different fishing techniques on the assessment of richness and composition of the fish fauna in selected aquatic environments. Six sites were selected to represent typical artificial (reservoirs and natural (intermittent streams environments and four different types of sampling gear were applied to each site during four occasions. The present study shows that when selecting sampling techniques to be used in aquatic systems in semi-arid Brazil, one must consider the objectives of the study, e.g. ecological or taxonomic, in order to decide on inclusion of rare species in the sampling population. Also, the effect of the sampling gear on natural abundances of fish must be considered given that some sampling techniques are highly detrimental to fish population numbers.

  15. Spatial and temporal structure of fish assemblages in an ''inverse estuary'', the Sine Saloum system (Senegal)

    Science.gov (United States)

    Simier, M.; Blanc, L.; Aliaume, C.; Diouf, P. S.; Albaret, J. J.

    2004-01-01

    As a consequence of the Sahelian drought, the Sine Saloum, a large estuarine system located in Senegal (West Africa), has become an "inverse estuary" since the late sixties, i.e. salinity increases upstream and reaches 100 in some places. To study the fish assemblages of such a modified system, a survey was conducted in 1992, collecting fish every two months with a purse seine at eight sites spread over the three main branches of the estuary. A total of 73 species belonging to 35 families were identified. Eight species comprised 97% of the total numbers of fish. The predominant species was a small clupeid, Sardinella maderensis, representing more than half of the total biomass and nearly 70% of the total number of fish. The spatio-temporal structure of the fish assemblages was studied using the STATIS-CoA method, which combines the multitable approach with the correspondence analysis method. Whatever the season, a strong spatial organization of fish assemblages was observed, mainly related to depth and salinity. Three types of assemblages were identified. In shallow water areas, fish assemblages were dominated by Mugilidae, Gerreidae and Cichlidae and were stable with time. In open water areas, large fluctuations in the species composition were observed, due to the occasional presence of large schools of pelagic species: in the southern area, where salinity and water transparency were the lowest, the main species were Ilisha africana, Brachydeuterus auritus and Chloroscombrus chrysurus, associated with a few Sciaenidae and Tetraodontidae, while the poorest areas were characterized by only two dominant species, S. maderensis and Scomberomorus tritor.

  16. Coliform Bacteria Monitoring in Fish Systems: Current Practices in Public Aquaria.

    Science.gov (United States)

    Culpepper, Erin E; Clayton, Leigh A; Hadfield, Catherine A; Arnold, Jill E; Bourbon, Holly M

    2016-06-01

    Public aquaria evaluate coliform indicator bacteria levels in fish systems, but the purpose of testing, testing methods, and management responses are not standardized, unlike with the coliform bacteria testing for marine mammal enclosures required by the U.S. Department of Agriculture. An online survey was sent to selected aquaria to document current testing and management practices in fish systems without marine mammals. The information collected included indicator bacteria species, the size and type of systems monitored, the primary purpose of testing, sampling frequency, test methods, the criteria for interpreting results, corrective actions, and management changes to limit human exposure. Of the 25 institutions to which surveys were sent, 19 (76%) responded. Fourteen reported testing for fecal indicator bacteria in fish systems. The most commonly tested indicator species were total (86%) and fecal (79%) coliform bacteria, which were detected by means of the membrane filtration method (64%). Multiple types and sizes of systems were tested, and the guidelines for testing and corrective actions were highly variable. Only three institutions performed additional tests to confirm the identification of indicator organisms. The results from this study can be used to compare bacterial monitoring practices and protocols in fish systems, as an aid to discussions relating to the accuracy and reliability of test results, and to help implement appropriate management responses. Received August 23, 2015; accepted December 29, 2015.

  17. Electronic tagging and integrated product intelligence

    Science.gov (United States)

    Swerdlow, Martin; Weeks, Brian

    1996-03-01

    The advent of 'intelligent,' electronic data bearing tags is set to revolutionize the way industrial and retail products are identified and tracked throughout their life cycles. The dominant system for unique identification today is the bar code, which is based on printed symbology and regulated by the International Article Numbering Association. Bar codes provide users with significant operational advantages and generate considerable added value to packaging companies, product manufacturers, distributors and retailers, across supply chains in many different sectors, from retailing, to baggage handling and industrial components, e.g., for vehicles or aircraft. Electronic tags offer the potential to: (1) record and store more complex data about the product or any modifications which occur during its life cycle; (2) access (and up-date) stored data in real time in a way which does not involve contact with the product or article; (3) overcome the limitations imposed by systems which rely on line-of-sight access to stored data. Companies are now beginning to consider how electronic data tags can be used, not only to improve the efficiency of their supply chain processes, but also to revolutionize the way they do business. This paper reviews the applications and business opportunities for electronic tags and outlines CEST's strategy for achieving an 'open' standard which will ensure that tags from different vendors can co-exist on an international basis.

  18. Fish Rhabdoviruses

    Science.gov (United States)

    Kurath, G.; Winton, J.

    2008-01-01

    Many important viral pathogens of fish are members of the family Rhabdoviridae. The viruses in this large group cause significant losses in populations of wild fish as well as among fish reared in aquaculture. Fish rhabdoviruses often have a wide host and geographic range, and infect aquatic animals in both freshwater and seawater. The fish rhabdoviruses comprise a diverse collection of isolates that can be placed in one of two quite different groups: isolates that are members of the established genusNovirhabdovirus, and those that are most similar to members of the genus Vesiculovirus. Because the diseases caused by fish rhabdoviruses are important to aquaculture, diagnostic methods for their detection and identification are well established. In addition to regulations designed to reduce the spread of fish viruses, a significant body of research has addressed methods for the control or prevention of diseases caused by fish rhabdoviruses, including vaccination. The number of reported fish rhabdoviruses continues to grow as a result of the expansion of aquaculture, the increase in global trade, the development of improved diagnostic methods, and heightened surveillance activities. Fish rhabdoviruses serve as useful components of model systems to study vertebrate virus disease, epidemiology, and immunology.

  19. Design and demonstration of a fish robot actuated by a SMA-driven actuation system

    Science.gov (United States)

    Le, Chan H.; Nguyen, Quang S.; Park, Hoon C.

    2010-04-01

    This paper presents a concept of a fish robot actuated by an SMA-based actuator. The bending-type actuator system is composed of a 0.1mm diameter SMA wire and a 0.5mm thick glass/epoxy strip. The SMA wire is installed to the bent composite strip. The actuator can produce about 200gf of blocking force and 3.5mm displacement at the center of the glass/epoxy strip. The bending motion of the actuator is converted into the tail-beat motion of a fish robot through a linkage system. The fish robot is evaluated by measuring the tail-beat angle, swimming speed and thrust produced by the fish robot. The tail-beat angle is about 20° and the maximum swimming speed is about 1.6cm/s. The measured thrust is about 0.4gf when the fish robot is operated at 0.9Hz.

  20. A DNA Mini-Barcoding System for Authentication of Processed Fish Products.

    Science.gov (United States)

    Shokralla, Shadi; Hellberg, Rosalee S; Handy, Sara M; King, Ian; Hajibabaei, Mehrdad

    2015-10-30

    Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences-mini-barcodes-for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species commonly used in processed fish products in North America. Six mini-barcode primer pairs targeting short (127-314 bp) fragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Administration (FDA) Seafood List. The mini-barcode primer pairs were then tested against 44 processed fish products representing a range of species and product types. Of the 44 products, 41 (93.2%) could be identified at the species or genus level. The greatest mini-barcoding success rate found with an individual primer pair was 88.6% compared to 20.5% success rate achieved by the full-length DNA barcode primers. Overall, this study presents a mini-barcoding system that can be used to identify a wide range of fish species in commercial products and may be utilized in high throughput DNA sequencing for authentication of heavily processed fish products.

  1. Use of Surveillance Systems in Detection of a Ciguatera Fish Poisoning Outbreak - Orange County, Florida, 2014.

    Science.gov (United States)

    Klekamp, Benjamin G; Bodager, Dean; Matthews, Sarah D

    2015-10-16

    What is already known on this topic? Ciguatera fish poisoning (CFP), caused by the ingestion of predatory reef-dwelling fish harboring ciguatoxins is one of the most commonly reported fish-associated marine intoxications. Ciguatoxin retains toxicity regardless of freezing or cooking. Prompt treatment can reduce debilitating neurologic symptoms that are associated with CFP.What is added by this report? Syndromic surveillance systems in Florida identified six adults with CFP following consumption of black grouper. Five patients sought medical attention; health care providers did not make a diagnosis of CFP or report the cases to public health authorities, and none of the patients received treatment. Close collaboration among several investigating agencies allowed traceback efforts to link black grouper consumed by all patients to a common international distributor.What are the implications for public health practice? Syndromic surveillance systems capable of detecting CFP are essential public health tools to identify outbreaks and enhance investigations. Medical and public health practitioners should be educated to inquire about recent fish consumption when evaluating patients with clinically compatible signs and symptoms to allow for prompt treatment, and report suspected CFP cases to public health authorities to facilitate source-food traceback efforts. Public education on avoidance of consumption of relatively large predatory reef fish species known to be from ciguatoxic-endemic areas might reduce the risk for CFP.

  2. Cortisol and melatonin in the cutaneous stress response system of fish.

    Science.gov (United States)

    Kulczykowska, Ewa; Kalamarz-Kubiak, Hanna; Gozdowska, Magdalena; Sokołowska, Ewa

    2018-04-01

    The stress hormone cortisol, together with antioxidants, melatonin (Mel) and its biologically active metabolites, 5-methoxykynuramines, including AFMK, set up a local stress response system in mammalian skin. Our in vitro study of the European flounder (Platichthys flesus) was designed to examine whether Mel and AFMK would respond to cortisol while a glucocorticoid is added to the incubation medium. The concentrations of cortisol in the incubation medium mimic plasma cortisol levels seen in fish exposed to different types of stresses such as handling, confinement, high density, food-deprivation or air-exposure. We measured Mel and AFMK in skin explants and culture media using high-performance liquid chromatography (HPLC) with fluorescence detection. We also analysed melanosome response (dispersion/aggregation) in the explants subjected to the different treatments. Cortisol stimulated the release of Mel and AFMK from skin explants in a dose-dependent manner. Melanosome dispersion and a darkening of the skin explants were observed after incubation with cortisol. This study is the first to demonstrate the interrelationship between cortisol and Mel/AFMK in fish skin. Our data strongly suggest that the cutaneous stress response system (CSRS) is present in fish. The question remains whether Mel, AFMK or cortisol are synthetized locally in fish skin and/or transported by the bloodstream. The presence of the CSRS should be taken into account during elaboration of new indicators of fish welfare both in aquaculture and in the wild. Copyright © 2018 Elsevier Inc. All rights reserved.

  3. Surface Acoustic Wave Tag-Based Coherence Multiplexing

    Science.gov (United States)

    Youngquist, Robert C. (Inventor); Malocha, Donald (Inventor); Saldanha, Nancy (Inventor)

    2016-01-01

    A surface acoustic wave (SAW)-based coherence multiplexing system includes SAW tags each including a SAW transducer, a first SAW reflector positioned a first distance from the SAW transducer and a second SAW reflector positioned a second distance from the SAW transducer. A transceiver including a wireless transmitter has a signal source providing a source signal and circuitry for transmitting interrogation pulses including a first and a second interrogation pulse toward the SAW tags, and a wireless receiver for receiving and processing response signals from the SAW tags. The receiver receives scrambled signals including a convolution of the wideband interrogation pulses with response signals from the SAW tags and includes a computing device which implements an algorithm that correlates the interrogation pulses or the source signal before transmitting against the scrambled signals to generate tag responses for each of the SAW tags.

  4. Isobaric Tags for Relative and Absolute Quantitation-Based Proteomic Analysis of Patent and Constricted Ductus Arteriosus Tissues Confirms the Systemic Regulation of Ductus Arteriosus Closure.

    Science.gov (United States)

    Hong, Haifa; Ye, Lincai; Chen, Huiwen; Xia, Yu; Liu, Yue; Liu, Jinfen; Lu, Yanan; Zhang, Haibo

    2015-08-01

    We aimed to evaluate global changes in protein expression associated with patency by undertaking proteomic analysis of human constricted and patent ductus arteriosus (DA). Ten constricted and 10 patent human DAs were excised from infants with ductal-dependent heart disease during surgery. Using isobaric tags for relative and absolute quantitation-based quantitative proteomics, 132 differentially expressed proteins were identified. Of 132 proteins, voltage-gated sodium channel 1.3 (SCN3A), myosin 1d (Myo1d), Rho GTPase activating protein 26 (ARHGAP26), and retinitis pigmentosa 1 (RP1) were selected for validation by Western blot and quantitative real-time polymerase chain reaction analyses. Significant upregulation of SCN3A, Myo1d, and RP1 messenger RNA, and protein levels was observed in the patent DA group (all P ≤ 0.048). ARHGAP26 messenger RNA and protein levels were decreased in patent DA tissue (both P ≤ 0.018). Immunohistochemistry analysis revealed that Myo1d, ARHGAP26, and RP1 were specifically expressed in the subendothelial region of constricted DAs; however, diffuse expression of these proteins was noted in the patent group. Proteomic analysis revealed global changes in the expression of proteins that regulate oxygen sensing, ion channels, smooth muscle cell migration, nervous system, immune system, and metabolism, suggesting a basis for the systemic regulation of DA patency by diverse signaling pathways, which will be confirmed in further studies.

  5. Ethical implications of implantable radiofrequency identification (RFID) tags in humans.

    Science.gov (United States)

    Foster, Kenneth R; Jaeger, Jan

    2008-08-01

    This article reviews the use of implantable radiofrequency identification (RFID) tags in humans, focusing on the VeriChip (VeriChip Corporation, Delray Beach, FL) and the associated VeriMed patient identification system. In addition, various nonmedical applications for implanted RFID tags in humans have been proposed. The technology offers important health and nonhealth benefits, but raises ethical concerns, including privacy and the potential for coercive implantation of RFID tags in individuals. A national discussion is needed to identify the limits of acceptable use of implantable RFID tags in humans before their use becomes widespread and it becomes too late to prevent misuse of this useful but ethically problematic technology.

  6. Tagging effects of passive integrated transponder and visual implant elastomer on the small-bodied white sands pupfish (Cyprinodon tularosa)

    Science.gov (United States)

    Peterson, Damon; Trantham, Randi B.; Trantham, Tulley G.; Caldwell, Colleen A.

    2018-01-01

    One of the greatest limiting factors of studies designed to obtain growth, movement, and survival in small-bodied fishes is the selection of a viable tag. The tag must be relatively small with respect to body size as to impart minimal sub-lethal effects on growth and mobility, as well as be retained throughout the life of the fish or duration of the study. Thus, body size of the model species becomes a major limiting factor; yet few studies have obtained empirical evidence of the minimum fish size and related tagging effects. The probability of surviving a tagging event was quantified in White Sands pupfish (Cyprinodon tularosa) across a range of sizes (19–60 mm) to address the hypothesis that body size predicts tagging survival. We compared tagging related mortality, individual taggers, growth, and tag retention in White Sands pupfish implanted with 8-mm passive integrated transponder (PIT), visual implant elastomer (VIE), and control (handled similarly, but no tag implantation) over a 75 d period. Initial body weight was a good predictor of the probability of survival in PIT- and VIE-tagged fish. As weight increased by 1 g, the fish were 4.73 times more likely to survive PIT-tag implantation compared to the control fish with an estimated suitable tagging size at 1.1 g (TL: 39.29 ± 0.41 mm). Likewise, VIE-tagged animals were 2.27 times more likely to survive a tagging event compared to the control group for every additional 1 g with an estimated size suitable for tagging of 0.9 g (TL: 36.9 ± 0.36 mm) fish. Growth rates of PIT- and VIE-tagged White Sands pupfish were similar to the control groups. This research validated two popular tagging methodologies in the White Sands pupfish, thus providing a valuable tool for characterizing vital rates in other small-bodied fishes.

  7. Fish reproduction

    National Research Council Canada - National Science Library

    Rocha, Maria João; Arukwe, Augustine; Kapoor, B. G

    2008-01-01

    ... of reproductive systems is essential for such studies. Fishes comprise over 28,000 species, with a remarkable variability in morphology, physiology and environmental adaptation. Knowledge on fish reproduction is scattered across numerous sources that shows a dynamic research field. The Editors believe it to be an opportune moment for a...

  8. Cattle grazing and fish recovery on US federal lands: can social-ecological systems science help?

    Science.gov (United States)

    Susan Charnley; Hannah Gosnell; Kendra L Wendel; Mary M Rowland; Michael J Wisdom

    2018-01-01

    In the western US, grazing management on federal lands containing habitat for fish species listed under the US Endangered Species Act (ESA) has sparked social conflict and litigation for decades. To date, the problem has been addressed through a top-down environmental governance system, but rangeland managers and grazing permittees now believe there is a need for more...

  9. Annual coded wire tag program, Washington: Missing production groups. Annual report for 1998

    International Nuclear Information System (INIS)

    Byrne, J.; Fuss, H.

    1999-01-01

    The Bonneville Power Administration (BPA) funds the ''Annual Coded Wire Tag Program--Missing Production Groups for Columbia River Hatcheries'' project. The WDFW project has three main objectives: (1) coded-wire tag at least one production group of each species at each Columbia Basin hatchery to enable evaluation of survival and catch distribution over time, (2) recover coded-wire tags from the snouts of fish tagged under objective 1 and estimate survival, contribution, and stray rates for each group, and (3) report the findings under objective 2 for all broods of chinook, and coho released from WDFW Columbia Basin hatcheries

  10. Product-service system method to measure sustainability level of traditional smoked fish processing industries

    Directory of Open Access Journals (Sweden)

    Purwaningsih Ratna

    2018-01-01

    Full Text Available Small Medium Enterprise’s (SME of traditional fish processing at Semarang, Central Java, Indonesia still focus their business on gain more profits. Sustainability aspect has not received enough attention yet. This study aims to review the sustainability level of SME smoked fish Semarang using product service system (PSS method. PSS consists of three dimensions (1 Environment, (2 Socio-cultural and (3 Economic. Each dimension consists of 6 criteria's. PSS not only assess the level of sustainability but also formulated the recommendation to increase the industries sustainability level. Sustainability assessment and recommendations formulation is guided by a check-list form. Then, the portfolio diagram used to select these recommendations according to its feasibility to be implemented and its importance for the industries. The result of sustainability assessment for traditional fish processing is 0.44, categorized as medium level. The recommendations for the environmental dimension are (1 use of liquid smoke on fish processing and (2 use of wastewater treatment with anaerobic ponds Recommendation for the socio-cultural dimension is use personal protective tool to reduce worker risk on safety and health. Recommendation for the economic dimension is used social media for product marketing and increasing the economic value of fish lung wastes. Recommendations are then illustrated in a diagram in the form of radar sustainability.

  11. Investigation and realization of an automatic device for the control and test of a photon tagging system

    International Nuclear Information System (INIS)

    Fallou, J.L.

    1987-12-01

    An intelligent control/test equipment for a monoenergetic photon production system was developed. The device enables simulations to be done outside experimental runs. Operation can be entirely automatic, or controlled by the experimenters. The device is modular and conforms to CAMAC standards. The architecture of the system is based around a local bus which comprises a central unit and its memory; a programmable pulse generator; a switching circuit to test and control the various paths; and a unit to communicate with the acquisition system [fr

  12. Genetic mating systems and reproductive natural histories of fishes: lessons for ecology and evolution.

    Science.gov (United States)

    Avise, John C; Jones, Adam G; Walker, DeEtte; DeWoody, J Andrew

    2002-01-01

    Fish species have diverse breeding behaviors that make them valuable for testing theories on genetic mating systems and reproductive tactics. Here we review genetic appraisals of paternity and maternity in wild fish populations. Behavioral phenomena quantified by genetic markers in various species include patterns of multiple mating by both sexes; frequent cuckoldry by males and rare cuckoldry by females in nest-tending species; additional routes to surrogate parentage via nest piracy and egg-thievery; egg mimicry by nest-tending males; brood parasitism by helper males in cooperative breeders; clutch mixing in oral brooders; kinship in schooling fry of broadcast spawners; sperm storage by dams in female-pregnant species; and sex-role reversal, polyandry, and strong sexual selection on females in some male-pregnant species. Additional phenomena addressed by genetic parentage analyses in fishes include clustered mutations, filial cannibalism, and local population size. All results are discussed in the context of relevant behavioral and evolutionary theory.

  13. Passive UHF RFID Tag for Multispectral Assessment

    Directory of Open Access Journals (Sweden)

    Pablo Escobedo

    2016-07-01

    Full Text Available This work presents the design, fabrication, and characterization of a passive printed radiofrequency identification tag in the ultra-high-frequency band with multiple optical sensing capabilities. This tag includes five photodiodes to cover a wide spectral range from near-infrared to visible and ultraviolet spectral regions. The tag antenna and circuit connections have been screen-printed on a flexible polymeric substrate. An ultra-low-power microcontroller-based switch has been included to measure the five magnitudes issuing from the optical sensors, providing a spectral fingerprint of the incident electromagnetic radiation from ultraviolet to infrared, without requiring energy from a battery. The normalization procedure has been designed applying illuminants, and the entire system was tested by measuring cards from a colour chart and sensing fruit ripening.

  14. Predicted and observed therapeutic dose exceedances of ionizable pharmaceuticals in fish plasma from urban coastal systems.

    Science.gov (United States)

    Scott, W Casan; Du, Bowen; Haddad, Samuel P; Breed, Christopher S; Saari, Gavin N; Kelly, Martin; Broach, Linda; Chambliss, C Kevin; Brooks, Bryan W

    2016-04-01

    Instream flows of the rapidly urbanizing watersheds and estuaries of the Gulf of Mexico in Texas (USA) are increasingly dominated by reclaimed waters. Though ionizable pharmaceuticals have received increasing attention in freshwaters, many research questions remain unanswered, particularly in tidally influenced urban coastal systems, which experience significant spatiotemporal variability in pH that influences bioavailability and bioaccumulation. The authors coupled fish plasma modeling of therapeutic hazard values with field monitoring of water chemistry variability and pharmaceutical occurrence to examine whether therapeutic hazards to fish existed within these urban coastal ecosystems and whether therapeutic hazards differed within and among coastal locations and seasons. Spatial and temporal fluctuations in pH within study sites altered the probability of encountering pharmaceutical hazards to fish. Significant water quality differences were consistently observed among traditional parameters and pharmaceuticals collected from surface and bottom waters, which are rarely sampled during routine surface water quality assessments. The authors then compared modeling predictions of fish plasma concentrations of pharmaceuticals to measured plasma levels from various field-collected fish species. Diphenhydramine and diltiazem were observed in plasma of multiple species, and diltiazem exceeded human therapeutic doses in largemouth bass, catfish, and mullet inhabiting these urban estuaries. Though the present study only examined a small number of target analytes, which represent a microcosm of the exposome of these fish, coastal systems are anticipated to be more strongly influenced by continued urbanization, altered instream flows, and population growth in the future. Unfortunately, aquatic toxicology information for diltiazem and many other pharmaceuticals is not available for marine and estuarine organisms, but such field observations suggest that potential adverse

  15. The use of tags and tag clouds to discern credible content in online health message forums.

    Science.gov (United States)

    O'Grady, Laura; Wathen, C Nadine; Charnaw-Burger, Jill; Betel, Lisa; Shachak, Aviv; Luke, Robert; Hockema, Stephen; Jadad, Alejandro R

    2012-01-01

    Web sites with health-oriented content are potentially harmful if inaccurate or inappropriate medical information is used to make health-related decisions. Checklists, rating systems and guidelines have been developed to help people determine what is credible, but recent Internet technologies emphasize applications that are collaborative in nature, including tags and tag clouds, where site users 'tag' or label online content, each using their own labelling system. Concepts such as the date, reference, author, testimonial and quotations are considered predictors of credible content. An understanding of these descriptive tools, how they relate to the depiction of credibility and how this relates to overall efforts to label data in relation to the semantic web has yet to emerge. This study investigates how structured (pre-determined) and unstructured (user-generated) tags and tag clouds with a multiple word search feature are used by participants to assess credibility of messages posted in online message forums. The targeted respondents were those using web sites message forums for disease self-management. We also explored the relevancy of our findings to the labelling or indexing of data in the context of the semantic web. Diabetes was chosen as the content area in this study, since (a) this is a condition with increasing prevalence and (b) diabetics have been shown to actively use the Internet to manage their condition. From January to March 2010 participants were recruited using purposive sampling techniques. A screening instrument was used to determine eligibility. The study consisted of a demographic and computer usage survey, a series of usability tests and an interview. We tested participants (N=22) on two scenarios, each involving tasks that assessed their ability to tag content and search using a tag cloud that included six structured credibility terms (statistics, date, reference, author, testimonial and quotations). MORAE Usability software (version 3

  16. Measuring and Predicting Tag Importance for Image Retrieval.

    Science.gov (United States)

    Li, Shangwen; Purushotham, Sanjay; Chen, Chen; Ren, Yuzhuo; Kuo, C-C Jay

    2017-12-01

    Textual data such as tags, sentence descriptions are combined with visual cues to reduce the semantic gap for image retrieval applications in today's Multimodal Image Retrieval (MIR) systems. However, all tags are treated as equally important in these systems, which may result in misalignment between visual and textual modalities during MIR training. This will further lead to degenerated retrieval performance at query time. To address this issue, we investigate the problem of tag importance prediction, where the goal is to automatically predict the tag importance and use it in image retrieval. To achieve this, we first propose a method to measure the relative importance of object and scene tags from image sentence descriptions. Using this as the ground truth, we present a tag importance prediction model to jointly exploit visual, semantic and context cues. The Structural Support Vector Machine (SSVM) formulation is adopted to ensure efficient training of the prediction model. Then, the Canonical Correlation Analysis (CCA) is employed to learn the relation between the image visual feature and tag importance to obtain robust retrieval performance. Experimental results on three real-world datasets show a significant performance improvement of the proposed MIR with Tag Importance Prediction (MIR/TIP) system over other MIR systems.

  17. The reaction of the immune system of fish to vaccination

    NARCIS (Netherlands)

    Lamers, C.H.J.

    1985-01-01

    The studies presented in this thesis deal with the effect of bacterial antigens of Yersinia ruckeri and Aeromonashydrophila on the immune system of carp. The antigens were administered by injection or by bath treatment. The effect on the immune system was studied by

  18. Annual coded wire tag program (Washington) missing production groups : annual report 2000; ANNUAL

    International Nuclear Information System (INIS)

    Dammers, Wolf; Mills, Robin D.

    2002-01-01

    The Bonneville Power Administration (BPA) funds the ''Annual Coded-wire Tag Program - Missing Production Groups for Columbia River Hatcheries'' project. The Washington Department of Fish and Wildlife (WDFW), Oregon Department of Fish and Wildlife (ODFW) and the United States Fish and Wildlife Service (USFWS) all operate salmon and steelhead rearing programs in the Columbia River basin. The intent of the funding is to coded-wire tag at least one production group of each species at each Columbia Basin hatchery to provide a holistic assessment of survival and catch distribution over time and to meet various measures of the Northwest Power Planning Council's (NWPPC) Fish and Wildlife Program. The WDFW project has three main objectives: (1) coded-wire tag at least one production group of each species at each Columbia Basin hatchery to enable evaluation of survival and catch distribution over time, (2) recover coded-wire tags from the snouts of fish tagged under objective 1 and estimate survival, contribution, and stray rates for each group, and (3) report the findings under objective 2 for all broods of chinook, and coho released from WDFW Columbia Basin hatcheries. Objective 1 for FY-00 was met with few modifications to the original FY-00 proposal. Under Objective 2, snouts containing coded-wire tags that were recovered during FY-00 were decoded. Under Objective 3, this report summarizes available recovery information through 2000 and includes detailed information for brood years 1989 to 1994 for chinook and 1995 to 1997 for coho

  19. Can Global Visual Features Improve Tag Recommendation for Image Annotation?

    Directory of Open Access Journals (Sweden)

    Oge Marques

    2010-08-01

    Full Text Available Recent advances in the fields of digital photography, networking and computing, have made it easier than ever for users to store and share photographs. However without sufficient metadata, e.g., in the form of tags, photos are difficult to find and organize. In this paper, we describe a system that recommends tags for image annotation. We postulate that the use of low-level global visual features can improve the quality of the tag recommendation process when compared to a baseline statistical method based on tag co-occurrence. We present results from experiments conducted using photos and metadata sourced from the Flickr photo website that suggest that the use of visual features improves the mean average precision (MAP of the system and increases the system's ability to suggest different tags, therefore justifying the associated increase in complexity.

  20. Slurry Ice as a Cooling System on 30 GT Fishing Vessel

    Directory of Open Access Journals (Sweden)

    Alam Baheramsyah

    2017-06-01

    Full Text Available Indonesia is the largest archipelago country in the world that has a sea area that is very spacious. Indonesian sea area is 5.8 million square kilometers and a coastline of 95 181 km has huge potential in the fisheries sector. In line with the need to further improve on the quality of the fish catch. One way to preserve fish is to use a slurry of ice. Slurry ice proved more effective preserving fishery products instead of using ice cubes. Ice slurry cooling system was designed and applied to the fishing vessel 30 GT. The cooling system uses a simple vapor compression system consists of five major components consisting of evaporator, condenser, compressor, and two pumps.In designing this system determined the type of refrigerant used in advance which type of refrigerant R-507a. Then do the design or selection of its main components. The design is only done on the evaporator. As for the other major components such as condensers, compressors, and pumps election in accordance with the specification of the power needed. After that dialakukan depiction of each system component. Then subsequently designing the laying of ice slurry cooling system components on a fishing vessel 30 GT.            Through calculations using simple thermodynamic equations obtained cooling load on this system amounted to 32.06 kW. Condenser with a power of 40 kW. Compressor with power 12 kW. Pump with capacity 10 m3 / h. With memepertimbangkan space left on the ship in the ice slurry system design on the main deck of the ship to the efficient use of space on board. The power requirements of the generator vessel increases due to the addition of ice slurry system components therefore do replacement generator into the generator with a power of 100 kW and penambahn fuel tank to 6,000 L.

  1. Fish communities of the Wilderness Lakes System in the southern Cape, South Africa

    Directory of Open Access Journals (Sweden)

    Alexis A. Olds

    2016-08-01

    Full Text Available The Wilderness Lakes System, a temporarily open and closed estuary with three associated lakes situated in the southern Cape region of South Africa, was sampled using a range of sampling gears to assess the fish community. A total of 25 species were sampled throughout the system, with the highest diversity in the Touw Estuary (23 species and the lowest in Langvlei (11 species. Estuary-associated marine species (13 species dominated species richness with smaller proportions of estuarine resident (7 species, freshwater (3 species and catadromous species (2 species. Estuarine resident species dominated the catch numerically. The size–class distribution of euryhaline marine species indicated that upon entering the Touw Estuary as juveniles, the fish move up the system towards Rondevlei where they appear to remain. Three freshwater species were recorded in the system, all of which are alien to the Wilderness Lakes System. Decreasing salinity in the upper lakes appears to be a driving factor in the distribution and increasing abundance of the freshwater fishes. Sampling followed a drought, with the system experiencing substantially increased levels of mouth closure compared to a similar study conducted in the 1980s. The timing of mouth opening and the degree of connectivity between the lakes influence the nursery function of the system as a whole. Management actions need to focus on improving ecological functioning of this system, in particular how mouth opening is managed, to facilitate nursery function and limit the establishment of invasive species. Conservation implications: Key management actions are required to improve fish recruitment potential into and within the system. These include maintenance of adequate marine inflow through adherence to artificial mouth breaching protocols and improving connectivity between the lakes through sediment removal from localised deposition points within the connecting channels.

  2. Antenna for passive RFID tags

    Science.gov (United States)

    Schiopu, Paul; Manea, Adrian; Cristea, Ionica; Grosu, Neculai; Vladescu, Marian; Craciun, Anca-Ileana; Craciun, Alexandru

    2015-02-01

    Minuscule devices, called RFID tags are attached to objects and persons and emit information which positioned readers may capture wirelessly. Many methods of identification have been used, but that of most common is to use a unique serial number for identification of person or object. RFID tags can be characterized as either active or passive [1,2]. Traditional passive tags are typically in "sleep" state until awakened by the reader's emitted field. In passive tags, the reader's field acts to charge the capacitor that powers the badge and this can be a combination of antenna and barcodes obtained with SAW( Surface Acoustic Wave) devices [1,2,3] . The antenna in an RFID tag is a conductive element that permits the tag to exchange data with the reader. The paper contribution are targeted to antenna for passive RFID tags. The electromagnetic field generated by the reader is somehow oriented by the reader antenna and power is induced in the tag only if the orientation of the tag antenna is appropriate. A tag placed orthogonal to the reader yield field will not be read. This is the reason that guided manufacturers to build circular polarized antenna capable of propagating a field that is alternatively polarized on all planes passing on the diffusion axis. Passive RFID tags are operated at the UHF frequencies of 868MHz (Europe) and 915MHz (USA) and at the microwave frequencies of 2,45 GHz and 5,8 GHz . Because the tags are small dimensions, in paper, we present the possibility to use circular polarization microstrip antenna with fractal edge [2].

  3. Nuclear studies with tagged photons

    International Nuclear Information System (INIS)

    Axel, P.

    1979-01-01

    First, the photon tagging technique will be described schematically, and a brief history of photon tagging will be given, including the 20 year development of this technique at Illinois. In the second part some typical operating conditions will be indicated for our tagged photon facility. The final section of this paper will illustrate some types of experiments by showing data obtained recently. (KBE) 891 KBE/KBE 892 ARA

  4. Soil-N tagging - a method for measurement of biological nitrogen fixation in cereal-legume intercropping system

    International Nuclear Information System (INIS)

    Patra, D.D.; Subbiah, B.V.; Sachdev, M.S.

    1985-01-01

    The quantitative estimates of atmospheric dinitrogen fixed by the legume crop and transferred to the associated cereal in cereal-legume intercropping system of maize-cowpea and wheat-gram using soil and fertilizer nitrogen labelling with 15 N have been reported. The estimates of N-fixation have been compared with the similar data from A-value method. Under field conditions sole cropped cowpea fixed 53.7 per cent of its total N uptake while as intercrop with maize fixed 43.5 per cent. Maize crop got 27.6 per cent of its total N uptake by transference of the nitrogen fixed by the intercropped cowpea. In the wheat-gram intercropping system the corresponding values under greenhouse conditions were 35.0, 44.8 and 20.2 per cent, respectively. (author)

  5. An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies

    OpenAIRE

    Frentiu, Francesca D; Adamski, Marcin; McGraw, Elizabeth A; Blows, Mark W; Chenoweth, Stephen F

    2009-01-01

    Abstract Background The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selection and traits involved in climatic adaptation along latitudinal gradients. Understanding the molecular genetic basis of such traits has been limited by a lack of genomic resources for thi...

  6. Buddy Tag CONOPS and Requirements.

    Energy Technology Data Exchange (ETDEWEB)

    Brotz, Jay Kristoffer [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Deland, Sharon M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-12-01

    This document defines the concept of operations (CONOPS) and the requirements for the Buddy Tag, which is conceived and designed in collaboration between Sandia National Laboratories and Princeton University under the Department of State Key VerificationAssets Fund. The CONOPS describe how the tags are used to support verification of treaty limitations and is only defined to the extent necessary to support a tag design. The requirements define the necessary functions and desired non-functional features of the Buddy Tag at a high level

  7. Flavour tagging performance in LHCb

    International Nuclear Information System (INIS)

    Grabalosa Gandara, Marc

    2009-01-01

    To do precise CP violation measurements, the best possible determination of the flavour of the B-meson is necessary. This report summarizes the flavour tagging performances for the LHCb experiment. The flavour tagging is obtained through a combination of several methods, based on different signatures. The use of control channels, which are decays to flavour-specific final states, will allow to determine the wrong tag fraction ω (the probability of a tag to be wrong), which can be used as an input for the determination of CKM unitarity triangle angles.

  8. Impacts of different rice-fish-prawn culture systems on yield of rice, fish and prawn and limnological conditions

    OpenAIRE

    Nahar, Ashfaqun

    2010-01-01

    An experiment was conducted to determine the impact of fish and prawn culture on some physicochemical parameters of water, weeds, benthos, and rice yield under simultaneous method for a period of 5 months from July to November 2007. The experiment was comprised of five individual treatments having three replicates for each. The treatments were: rice combined with fish and regular urea fertilization (treatment І, T1), rice combined with prawn and regular urea fertilization (treatment ІI, T2), ...

  9. Depth- and range-dependent variation in the performance of aquatic telemetry systems: understanding and predicting the susceptibility of acoustic tag-receiver pairs to close proximity detection interference.

    Science.gov (United States)

    Scherrer, Stephen R; Rideout, Brendan P; Giorli, Giacomo; Nosal, Eva-Marie; Weng, Kevin C

    2018-01-01

    Passive acoustic telemetry using coded transmitter tags and stationary receivers is a popular method for tracking movements of aquatic animals. Understanding the performance of these systems is important in array design and in analysis. Close proximity detection interference (CPDI) is a condition where receivers fail to reliably detect tag transmissions. CPDI generally occurs when the tag and receiver are near one another in acoustically reverberant settings. Here we confirm transmission multipaths reflected off the environment arriving at a receiver with sufficient delay relative to the direct signal cause CPDI. We propose a ray-propagation based model to estimate the arrival of energy via multipaths to predict CPDI occurrence, and we show how deeper deployments are particularly susceptible. A series of experiments were designed to develop and validate our model. Deep (300 m) and shallow (25 m) ranging experiments were conducted using Vemco V13 acoustic tags and VR2-W receivers. Probabilistic modeling of hourly detections was used to estimate the average distance a tag could be detected. A mechanistic model for predicting the arrival time of multipaths was developed using parameters from these experiments to calculate the direct and multipath path lengths. This model was retroactively applied to the previous ranging experiments to validate CPDI observations. Two additional experiments were designed to validate predictions of CPDI with respect to combinations of deployment depth and distance. Playback of recorded tags in a tank environment was used to confirm multipaths arriving after the receiver's blanking interval cause CPDI effects. Analysis of empirical data estimated the average maximum detection radius (AMDR), the farthest distance at which 95% of tag transmissions went undetected by receivers, was between 840 and 846 m for the deep ranging experiment across all factor permutations. From these results, CPDI was estimated within a 276.5 m radius of the

  10. Rooted Rights Systems in Turbulent Water: The Dynamics of Collective Fishing Rights in La Albufera, Valencia, Spain

    NARCIS (Netherlands)

    Boelens, R.A.; Claudin, V.

    2015-01-01

    Valencia's Albufera Lake is a wetlands area where different sociolegal systems interact. Its El Palmar community is governed by customary laws for fishing and territorial control. These exist alongside, yet in tension with, governmental laws. This article examines the dynamics of fishing rights,

  11. Rooted rights systems in turbulent waters: the dynamics of collective fishing rights in La Albufera, Valencia, Spain

    NARCIS (Netherlands)

    Boelens, R.; Claudin, V.

    2015-01-01

    Valencia's Albufera Lake is a wetlands area where different sociolegal systems interact. Its El Palmar community is governed by customary laws for fishing and territorial control. These exist alongside, yet in tension with, governmental laws. This article examines the dynamics of fishing rights,

  12. Fish welfare assurance system: initial steps to set up an effective tool to safeguard and monitor farmed fish welfare at a company level

    NARCIS (Netherlands)

    Vis, van de J.W.; Poelman, M.; Lambooij, E.; Bégout, M.L.; Pilarczyk, M.

    2012-01-01

    The objective was to take a first step in the development of a process-oriented quality assurance (QA) system for monitoring and safeguarding of fish welfare at a company level. A process-oriented approach is focused on preventing hazards and involves establishment of critical steps in a process

  13. Emotional Mining: Tagging Emoticons to Online News

    Science.gov (United States)

    Kasinathan, Vinothini; Mustapha, Aida; Zhi Yong, Lee; Aida Zamnah, Z. A.

    2017-08-01

    This paper presents an emotion mining system, which assigns emoticons to newspaper articles into a pre-defined emotion category based on the underlying emotion in the news. Next, the system makes recommendation to the reader by tagging the news headline with the respective emoticons. Users are then able to decide whether to read the news based on the emoticons provided. The system also provides a filter for the users to choose the category of news to read following the emoticons.

  14. Serotype determination of Salmonella by xTAG assay.

    Science.gov (United States)

    Zheng, Zhibei; Zheng, Wei; Wang, Haoqiu; Pan, Jincao; Pu, Xiaoying

    2017-10-01

    Currently, no protocols or commercial kits are available to determine the serotypes of Salmonella by using Luminex MAGPIX®. In this study, an xTAG assay for serotype determination of Salmonella suitable for Luminex MAGPIX® is described and 228 Salmonella isolates were serotype determined by this xTAG assay. The xTAG assay consists of two steps: 1) Multiplex PCR to amplify simultaneously O, H and Vi antigen genes of Salmonella, and 2) Magplex-TAG™ microsphere hybridization to identify accurately the specific PCR products of different antigens. Compared with the serotyping results of traditional serum agglutination test, the sensitivity and specificity of the xTAG assay were 95.1% and 100%, respectively. The agreement rate of these two assays was 95.2%. Compared with Luminex xMAP® Salmonella Serotyping Assay (SSA) kit, the advantages of this xTAG assay are: First, the magnetic beads make it applicable to both the Luminex®100/200™ and MAGPIX® systems. Second, only primers rather than both primers and probes are needed in the xTAG assay, and the process of coupling antigen-specific oligonucleotide probes to beads is circumvented, which make the xTAG assay convenient to be utilized by other laboratories. The xTAG assay may serve as a rapid alternative or complementary method for traditional Salmonella serotyping tests, especially for laboratories that utilize the MAGPIX® systems. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Long-term GPS tracking of ocean sunfish Mola mola offers a new direction in fish monitoring.

    Directory of Open Access Journals (Sweden)

    David W Sims

    Full Text Available Satellite tracking of large pelagic fish provides insights on free-ranging behaviour, distributions and population structuring. Up to now, such fish have been tracked remotely using two principal methods: direct positioning of transmitters by Argos polar-orbiting satellites, and satellite relay of tag-derived light-level data for post hoc track reconstruction. Error fields associated with positions determined by these methods range from hundreds of metres to hundreds of kilometres. However, low spatial accuracy of tracks masks important details, such as foraging patterns. Here we use a fast-acquisition global positioning system (Fastloc GPS tag with remote data retrieval to track long-term movements, in near real time and position accuracy of <70 m, of the world's largest bony fish, the ocean sunfish Mola mola. Search-like movements occurred over at least three distinct spatial scales. At fine scales, sunfish spent longer in highly localised areas with faster, straighter excursions between them. These 'stopovers' during long-distance movement appear consistent with finding and exploiting food patches. This demonstrates the feasibility of GPS tagging to provide tracks of unparalleled accuracy for monitoring movements of large pelagic fish, and with nearly four times as many locations obtained by the GPS tag than by a conventional Argos transmitter. The results signal the potential of GPS-tagged pelagic fish that surface regularly to be detectors of resource 'hotspots' in the blue ocean and provides a new capability for understanding large pelagic fish behaviour and habitat use that is relevant to ocean management and species conservation.

  16. Engineering the ATLAS TAG Browser

    CERN Document Server

    Zhang, Q; The ATLAS collaboration

    2011-01-01

    ELSSI is a web-based event metadata (TAG) browser and event-level selection service for ATLAS. TAGs from all ATLAS physics and Monte Carlo data sets are routinely loaded into Oracle databases as an integral part of event processing. As data volumes increase, more and more sites are joining the distributed TAG data hosting topology[1]. Meanwhile, TAG content and database schemata continue to evolve as new user requirements and additional sources of metadata emerge. All of this has posed many challenges to the development of ELSSI, which must support vast amounts of TAG data while source, content, geographic locations, and user query patterns may change over time. In this paper, we describe some of the challenges encountered in the process of developing ELSSI, and the software engineering strategies adopted to address those challenges. Approaches to management of access to data, browsing, data rendering, query building, query validation, execution, connection management, and communication with auxiliary service...

  17. Engineering the ATLAS TAG Browser

    CERN Document Server

    Zhang, Q; The ATLAS collaboration

    2011-01-01

    ELSSI is a web-based event metadata (TAG) browser and event-level selection service for ATLAS. TAGs from all ATLAS physics and Monte Carlo data sets are routinely loaded into Oracle databases as an integral part of event processing. As data volumes increase, more and more sites are joining the distributed TAG data hosting topology. Meanwhile, TAG content and database schemata continue to evolve as new user requirements and additional sources of metadata emerge. All of this has posed many challenges to the development of ELSSI, which must support vast amounts of TAG data while source, content, geographic locations, and user query patterns may change over time. In this paper, we describe some of the challenges encountered in the process of developing ELSSI, and the software engineering strategies adopted to address those challenges. Approaches to management of access to data, browsing, data rendering, query building, query validation, execution, connection management, and communication with auxiliary services a...

  18. 200-MeV bremsstrahlung tagged photon beams at Sendai

    International Nuclear Information System (INIS)

    Hirose, K.; Chiba, M.; Inoue, M.; Kanda, H.; Kimura, R.; Kino, K.; Kobayashi, Y.; Konno, O.; Maeda, K.; Miyase, H.; Miyamoto, A.; Ohtsuki, T.; Saito, A.; Suda, T.; Takahashi, K.; Tamae, T.; Terasaki, Y.; Terasawa, T.; Tsubota, H.; Tsuruta, T.; Utoyama, M.; Yuuki, H.; Yamaguchi, Y.; Yamazaki, H.

    2006-01-01

    A new beam line for photonuclear reaction experiments using tagged photons has been constructed to take advantage of the completion of the 1.2-GeV STretcher Booster (STB) ring at the Laboratory of Nuclear Science (LNS), Tohoku University. A photon tagging system was installed at the end of the new beam line. It provides bremsstrahlung tagged photon beams in an energy range from 0.2E 0 to 0.8E 0 MeV at the incident electron energy E 0 with an energy resolution of ΔE/E∼10 -2 . The tagged photon intensity I= 6 photons/s is available for typical photonuclear reaction experiments. We introduce the basic parameters of the tagged photons by showing the commissioning data

  19. Three Kinds of Fish

    DEFF Research Database (Denmark)

    Høst, Jeppe Engset

    2012-01-01

    There are three kinds of fish. Fish you were given, fish you bought and fish you lease. This might sound a bit odd, but it is nevertheless the basis for the activities of Danish commercial fishers since the introduction of transferable fishing concessions (TFCs) in 2007. In the current 2012 reform...... of market based systems are wild speculation, concentration and monopolization of fishing access and subsequent leasing with fishing communities and new entrants very likely being worse off (see for example the chapter “From fishing rights to financial derivatives” is this volume or Olson 2011; Sumaila 2010...... will examine five Danish fishing operations and discuss how they have reacted in different ways to the newly introduced system of transferable fishing concessions. By introducing TFCs as a solution to fleet overcapacity, the EU Commission will also be introducing a system where buying, selling and leasing...

  20. Endocrine and Local IGF-I in the Bony Fish Immune System.

    Science.gov (United States)

    Franz, Anne-Constance; Faass, Oliver; Köllner, Bernd; Shved, Natallia; Link, Karl; Casanova, Ayako; Wenger, Michael; D'Cotta, Helena; Baroiller, Jean-François; Ullrich, Oliver; Reinecke, Manfred; Eppler, Elisabeth

    2016-01-26

    A role for GH and IGF-I in the modulation of the immune system has been under discussion for decades. Generally, GH is considered a stimulator of innate immune parameters in mammals and teleost fish. The stimulatory effects in humans as well as in bony fish often appear to be correlated with elevated endocrine IGF-I (liver-derived), which has also been shown to be suppressed during infection in some studies. Nevertheless, data are still fragmentary. Some studies point to an important role of GH and IGF-I particularly during immune organ development and constitution. Even less is known about the potential relevance of local (autocrine/paracrine) IGF-I within adult and developing immune organs, and the distinct localization of IGF-I in immune cells and tissues of mammals and fish has not been systematically defined. Thus far, IGF-I has been localized in different mammalian immune cell types, particularly macrophages and granulocytes, and in supporting cells, but not in T-lymphocytes. In the present study, we detected IGF-I in phagocytic cells isolated from rainbow trout head kidney and, in contrast to some findings in mammals, in T-cells of a channel catfish cell line. Thus, although numerous analogies among mammals and teleosts exist not only for the GH/IGF-system, but also for the immune system, there are differences that should be further investigated. For instance, it is unclear whether the primarily reported role of GH/IGF-I in the innate immune response is due to the lack of studies focusing on the adaptive immune system, or whether it truly preferentially concerns innate immune parameters. Infectious challenges in combination with GH/IGF-I manipulations are another important topic that has not been sufficiently addressed to date, particularly with respect to developmental and environmental influences on fish growth and health.

  1. How biological (fish) noise affects the performance of shallow water passive array system

    Digital Repository Service at National Institute of Oceanography (India)

    Fernandes, W.A.; Chakraborty, B.; Haris, K.; Vijayakumar, K.; Sundar, D.; Luis, R.A.A.; Mahanty, M.M.; Latha, G.

    =UTF-8 How biological (fish) noise affects the performance of shallow water passive array system William Fernandes, Bishwajit Chakraborty, K. Haris, K. Vijaykumar, D. Sundar, R.A.A. Luis CSIR-National Institute of Oceanography, Dona Paula... source distribution as well as the environmental parameters (i.e., water depth, sound speed profile, and seafloor properties). In a waveguide bounded by sea surface and seabed, multipath propagation prevails and the spatial structure of the noise...

  2. Endocrine and Local IGF-I in the Bony Fish Immune System

    Directory of Open Access Journals (Sweden)

    Anne-Constance Franz

    2016-01-01

    Full Text Available A role for GH and IGF-I in the modulation of the immune system has been under discussion for decades. Generally, GH is considered a stimulator of innate immune parameters in mammals and teleost fish. The stimulatory effects in humans as well as in bony fish often appear to be correlated with elevated endocrine IGF-I (liver-derived, which has also been shown to be suppressed during infection in some studies. Nevertheless, data are still fragmentary. Some studies point to an important role of GH and IGF-I particularly during immune organ development and constitution. Even less is known about the potential relevance of local (autocrine/paracrine IGF-I within adult and developing immune organs, and the distinct localization of IGF-I in immune cells and tissues of mammals and fish has not been systematically defined. Thus far, IGF-I has been localized in different mammalian immune cell types, particularly macrophages and granulocytes, and in supporting cells, but not in T-lymphocytes. In the present study, we detected IGF-I in phagocytic cells isolated from rainbow trout head kidney and, in contrast to some findings in mammals, in T-cells of a channel catfish cell line. Thus, although numerous analogies among mammals and teleosts exist not only for the GH/IGF-system, but also for the immune system, there are differences that should be further investigated. For instance, it is unclear whether the primarily reported role of GH/IGF-I in the innate immune response is due to the lack of studies focusing on the adaptive immune system, or whether it truly preferentially concerns innate immune parameters. Infectious challenges in combination with GH/IGF-I manipulations are another important topic that has not been sufficiently addressed to date, particularly with respect to developmental and environmental influences on fish growth and health.

  3. Fish Welfare in Aquaponic Systems: Its Relation to Water Quality with an Emphasis on Feed and Faeces—A Review

    Directory of Open Access Journals (Sweden)

    Hijran Yavuzcan Yildiz

    2017-01-01

    Full Text Available Aquaponics is the combination of aquaculture (fish and hydroponic cultivation of plants. This review examines fish welfare in relation to rearing water quality, fish feed and fish waste and faeces to develop a sustainable aquaponic system where the co-cultured organisms, fish, bacteria in biofilters and plants, should be considered holistically in all aquaponics operations. Water quality parameters are the primary environmental consideration for optimizing aquaponic production and for directly impacting fish welfare/health issues and plant needs. In aquaponic systems, the uptake of nutrients should be maximised for the healthy production of the plant biomass but without neglecting the best welfare conditions for the fish in terms of water quality. Measures to reduce the risks of the introduction or spread of diseases or infection and to increase biosecurity in aquaponics are also important. In addition, the possible impacts of allelochemicals, i.e., chemicals released by the plants, should be taken into account. Moreover, the effect of diet digestibility, faeces particle size and settling ratio on water quality should be carefully considered. As available information is very limited, research should be undertaken to better elucidate the relationship between appropriate levels of minerals needed by plants, and fish metabolism, health and welfare. It remains to be investigated whether and to what extent the concentrations of suspended solids that can be found in aquaponic systems can compromise the health of fish. Water quality, which directly affects fish health and well-being, is the key factor to be considered in all aquaponic systems.

  4. 48 CFR 552.211-92 - Radio Frequency Identification (RFID) using passive tags.

    Science.gov (United States)

    2010-10-01

    ... Identification (RFID) using passive tags. 552.211-92 Section 552.211-92 Federal Acquisition Regulations System... Provisions and Clauses 552.211-92 Radio Frequency Identification (RFID) using passive tags. As prescribed in 511.204(b)(11), insert the following clause: Radio Frequency Identification (RFID) Using Passive Tags...

  5. Measuring the Interference at an RFID Tag: Where Does It Have an Impact?

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund

    2011-01-01

    In this paper we consider reader collisions in an RFID system, especially how interference impacts the ability of a passive UHF tag to respond. We propose two innovative applications for using interference: 1) Blocking a tag response, and 2) cooperative reading of a tag. In order to investigate...

  6. Bidirectional communication in an HF hybrid organic/solution-processed metal-oxide RFID tag

    NARCIS (Netherlands)

    Myny, K.; Rockelé, M.; Chasin, A.; Pham, D.V.; Steiger, J.; Botnaras, S.; Weber, D.; Herold, B.; Ficker, J.; Van Putten, B.D.; Gelinck, G.H.; Genoe, J.; Dehaene, W.; Heremans, P.

    2014-01-01

    A bidirectional communication protocol allows radio-frequency identification (RFID) tags to have readout of multiple tags in the RF field without collision of data. In this paper, we realized bidirectional communication between a reader system and thin-film RFID tag by introducing a novel protocol

  7. Cobalt Chloride Treatment Used to Ablate the Lateral Line System Also Impairs the Olfactory System in Three Freshwater Fishes.

    Directory of Open Access Journals (Sweden)

    Julie M Butler

    Full Text Available Fishes use multimodal signals during both inter- and intra-sexual displays to convey information about their sex, reproductive state, and social status. These complex behavioral displays can include visual, auditory, olfactory, tactile, and hydrodynamic signals, and the relative role of each sensory channel in these complex multi-sensory interactions is a common focus of neuroethology. The mechanosensory lateral line system of fishes detects near-body water movements and is implicated in a variety of behaviors including schooling, rheotaxis, social communication, and prey detection. Cobalt chloride is commonly used to chemically ablate lateral line neuromasts, thereby eliminating water-movement cues to test for mechanosensory-mediated behavioral functions. However, cobalt acts as a nonspecific calcium channel antagonist and could potentially disrupt function of all superficially located sensory receptor cells, including those for chemosensing. Here, we examined whether CoCl2 treatment used to ablate the lateral line system also impairs olfaction in three freshwater fishes, the African cichlid fish Astatotilapia burtoni, goldfish Carassius auratus, and the Mexican blind cavefish Astyanax mexicanus. To examine the impact of CoCl2 on the activity of peripheral receptors, we quantified DASPEI fluorescence intensity of the olfactory epithelium from fish exposed to control and CoCl2 solutions. In addition, we examined brain activation in olfactory processing regions of A. burtoni immersed in either control or cobalt solutions. All three species exposed to CoCl2 had decreased DASPEI staining of the olfactory epithelium, and in A. burtoni, cobalt treatment caused reduced neural activation in olfactory processing regions of the brain. To our knowledge this is the first empirical evidence demonstrating that the same CoCl2 treatment used to ablate the lateral line system also impairs olfactory function. These data have important implications for the use of

  8. Fundamental statistical features and self-similar properties of tagged networks

    International Nuclear Information System (INIS)

    Palla, Gergely; Farkas, Illes J; Pollner, Peter; Vicsek, Tamas; Derenyi, Imre

    2008-01-01

    We investigate the fundamental statistical features of tagged (or annotated) networks having a rich variety of attributes associated with their nodes. Tags (attributes, annotations, properties, features, etc) provide essential information about the entity represented by a given node, thus, taking them into account represents a significant step towards a more complete description of the structure of large complex systems. Our main goal here is to uncover the relations between the statistical properties of the node tags and those of the graph topology. In order to better characterize the networks with tagged nodes, we introduce a number of new notions, including tag-assortativity (relating link probability to node similarity), and new quantities, such as node uniqueness (measuring how rarely the tags of a node occur in the network) and tag-assortativity exponent. We apply our approach to three large networks representing very different domains of complex systems. A number of the tag related quantities display analogous behaviour (e.g. the networks we studied are tag-assortative, indicating possible universal aspects of tags versus topology), while some other features, such as the distribution of the node uniqueness, show variability from network to network allowing for pin-pointing large scale specific features of real-world complex networks. We also find that for each network the topology and the tag distribution are scale invariant, and this self-similar property of the networks can be well characterized by the tag-assortativity exponent, which is specific to each system.

  9. Development and Evaluation of Passive Integrated Transponder Tag Technology, 2000-2002.

    Energy Technology Data Exchange (ETDEWEB)

    Downing, Sandra L.; Prentice, Earl F.; Nunnallee, Edmund P. [National Marine Fisheries Service

    2009-04-03

    Since 1984, the National Marine Fisheries Service (NMFS) in cooperation with the Bonneville Power Administration (BPA) has conducted a research project to develop and evaluate technology for passive-integrated-transponder tags (PIT tags) throughout the Columbia River Basin (CRB). Work conducted as part of this project between October 2000 and September 2002 (FY01 and FY02) was divided into seven individual elements, which are covered separately in this report. The efforts by personnel associated with this project have produced and will continue to produce products that aid resource stakeholders in assessing the effectiveness of actions taken to enhance the survival of juvenile and adult salmonids. These products and their uses include: (1) Survival and migration timing information on stocks to evaluate water management strategies and fish passage/collection facilities; (2) Data needed for the management and restoration of salmonids and other fish stocks listed under the Endangered Species Act (ESA); (3) Information required for the management of multiple species in a variety of habitats; and (4) Tools that enable fisheries researchers and managers to address previously unanswerable questions and critical uncertainties These products are also used in genetic, physiology, behavior, and captive broodstock research on endangered species. The continued development of PIT-tag technology will enable researchers and fisheries managers to address issues expressed in both of NMFS biological opinions for operation of the Federal Columbia River Power System (FCRPS)(NMFS 1995a, 2000) and the proposed Snake River Recovery Plan (NMFS 1995b; tasks 2.1.d, 2.3.b.4, 2.4.a, 2.6.c.2, and 2.9.d).

  10. 1994 Annual Status Report. A Summary of Fish Data in Six Reaches of the Upper Mississippi River System

    National Research Council Canada - National Science Library

    Gutreuter, Steve

    1997-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 2,653 collections of fishes from stratified random sad permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during 1994...

  11. 1991 Annual Status Report. A Summary of Fish Data in Six Reaches of the Upper Mississippi River System

    National Research Council Canada - National Science Library

    Gutreuter, Steve

    1998-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 2,653 collections of fishes from stratified random and permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during 1991...

  12. 1996 Annual Status Report. A Summary of Fish Data in Six Reaches of the Upper Mississippi River System

    National Research Council Canada - National Science Library

    Burkhardt, Randy

    1997-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 2,378 collections of fishes from stratified random and permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during 1996...

  13. 1992 Annual Status Report: A Summary of Fish Data in Six Reaches of the Upper Mississippi River System

    National Research Council Canada - National Science Library

    Gutreuter, Steve

    1997-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 2,221 collections of fishes from stratified random and permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during I 992...

  14. 1997 Annual Status Report A Summary of Fish Data in Six Reaches of The Upper Mississippi River System

    National Research Council Canada - National Science Library

    Burkhardt, Randy

    1998-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 2,797 collections of fishes from stratified random and permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during 1997...

  15. 1998 Annual Status Report: A Summary of Fish Data in Six Reaches of the Upper Mississippi River System

    National Research Council Canada - National Science Library

    Burkhardt, Randy

    2000-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 2,664 collections of fishes from stratified random and permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during 1998...

  16. 1995 Annual Status Report. A Summary of Fish Data in Six Reaches of the Upper Mississippi River System

    National Research Council Canada - National Science Library

    Gutreuter, Steve

    1997-01-01

    The Long Term Resource Monitoring Program (LTRMP) completed 2,723 collections of fishes from stratified random and permanently fixed sampling locations in six study reaches of the Upper Mississippi River System during 1995...

  17. Cyanohepatotoxins influence on the neuroendocrine and immune systems in fish - a short review.

    Science.gov (United States)

    Sieroslawska, Anna; Rymuszka, Anna

    2009-01-01

    Cyanotoxins are the metabolites of cyanobacteria, belonging to different chemical groups and of diverse mechanisms of toxicity. Generally, they are divided into hepatotoxins, neurotoxins and dermatotoxins/irritant toxins. There is a growing evidence, that besides the above mentioned toxicity, exposure to cyanotoxins may also induce other effects, among others the disruption of neuroendocrine and immune systems. The purpose of that paper is to sum up the current information obtained from the literature and from our own studies about the influence of cyanohepatotoxins on neuroendocrine and immune systems of fish. From the presented data it appears, that microcystins, nodularin and cylindrospermopsin, except for their hepatotoxic activity, are potent to exert such effects as HPI axis activation resulting in physiological and behavioural changes, disturbances in thyroid hormones release/metabolism, as well as impairment of immune responses in fish. However the studies in that area are still incomplete and many questions remain to be answered, especially what consequences for fish population health status it brings.

  18. Modified Atmosphere Systems and Shelf Life Extension of Fish and Fishery Products

    Science.gov (United States)

    DeWitt, Christina A. Mireles; Oliveira, Alexandra C.M.

    2016-01-01

    This review aims at summarizing the findings of studies published over the past 15 years on the application of modified atmosphere (MA) systems for shelf life extension of fish and fishery products. This review highlights the importance of CO2 in the preservation of seafood products, and underscores the benefits of combining MA technology with product storage in the superchilled temperature range. It is generally accepted that MA technology cannot improve product quality and should not be utilized as a substitute for good sanitation and strict temperature control. Benefits derived from application of MA, however, can significantly impact preservation of product quality and it subsequent shelf-life. For this reason, this review is the first of its kind to propose detailed handling and quality guidelines for fresh fish to realize the maximum benefit of MA technology. PMID:28231143

  19. Natural shorelines promote the stability of fish communities in an urbanized coastal system.

    Directory of Open Access Journals (Sweden)

    Steven B Scyphers

    Full Text Available Habitat loss and fragmentation are leading causes of species extinctions in terrestrial, aquatic and marine systems. Along coastlines, natural habitats support high biodiversity and valuable ecosystem services but are often replaced with engineered structures for coastal protection or erosion control. We coupled high-resolution shoreline condition data with an eleven-year time series of fish community structure to examine how coastal protection structures impact community stability. Our analyses revealed that the most stable fish communities were nearest natural shorelines. Structurally complex engineered shorelines appeared to promote greater stability than simpler alternatives as communities nearest vertical walls, which are among the most prevalent structures, were most dissimilar from natural shorelines and had the lowest stability. We conclude that conserving and restoring natural habitats is essential for promoting ecological stability. However, in scenarios when natural habitats are not viable, engineered landscapes designed to mimic the complexity of natural habitats may provide similar ecological functions.

  20. Quantum tagging for tags containing secret classical data

    International Nuclear Information System (INIS)

    Kent, Adrian

    2011-01-01

    Various authors have considered schemes for quantum tagging, that is, authenticating the classical location of a classical tagging device by sending and receiving quantum signals from suitably located distant sites, in an environment controlled by an adversary whose quantum information processing and transmitting power is potentially unbounded. All of the schemes proposed elsewhere in the literature assume that the adversary is able to inspect the interior of the tagging device. All of these schemes have been shown to be breakable if the adversary has unbounded predistributed entanglement. We consider here the case in which the tagging device contains a finite key string shared with distant sites but kept secret from the adversary, and show this allows the location of the tagging device to be authenticated securely and indefinitely. Our protocol relies on quantum key distribution between the tagging device and at least one distant site, and demonstrates a new practical application of quantum key distribution. It also illustrates that the attainable security in position-based cryptography can depend crucially on apparently subtle details in the security scenario considered.

  1. Meet the surrogate fish

    International Nuclear Information System (INIS)

    Johnson, Bob; Neitzel, Duane; Moxon, Suzanne

    1999-01-01

    This article gives details of the US Department of Energy's innovative research into the development of a sensor system that will work as a surrogate fish to provide information to aid the design of fish-friendly turbines for hydroelectric power plants. The selection of the dams for the testing of sensor fish, the release and recovery of the sensor fish, the recording of the physical forces exerted on fish as they pass through the turbines, and use of the information gathered to build more sensor fish are discussed. Fish investigations conducted at the Pacific Northwest National Laboratory are briefly described. (UK)

  2. Fish invasions in the world's river systems: when natural processes are blurred by human activities.

    Directory of Open Access Journals (Sweden)

    Fabien Leprieur

    2008-02-01

    Full Text Available Because species invasions are a principal driver of the human-induced biodiversity crisis, the identification of the major determinants of global invasions is a prerequisite for adopting sound conservation policies. Three major hypotheses, which are not necessarily mutually exclusive, have been proposed to explain the establishment of non-native species: the "human activity" hypothesis, which argues that human activities facilitate the establishment of non-native species by disturbing natural landscapes and by increasing propagule pressure; the "biotic resistance" hypothesis, predicting that species-rich communities will readily impede the establishment of non-native species; and the "biotic acceptance" hypothesis, predicting that environmentally suitable habitats for native species are also suitable for non-native species. We tested these hypotheses and report here a global map of fish invasions (i.e., the number of non-native fish species established per river basin using an original worldwide dataset of freshwater fish occurrences, environmental variables, and human activity indicators for 1,055 river basins covering more than 80% of Earth's surface. First, we identified six major invasion hotspots where non-native species represent more than a quarter of the total number of species. According to the World Conservation Union, these areas are also characterised by the highest proportion of threatened fish species. Second, we show that the human activity indicators account for most of the global variation in non-native species richness, which is highly consistent with the "human activity" hypothesis. In contrast, our results do not provide support for either the "biotic acceptance" or the "biotic resistance" hypothesis. We show that the biogeography of fish invasions matches the geography of human impact at the global scale, which means that natural processes are blurred by human activities in driving fish invasions in the world's river systems

  3. Potential for Combined Biocontrol Activity against Fungal Fish and Plant Pathogens by Bacterial Isolates from a Model Aquaponic System

    Directory of Open Access Journals (Sweden)

    Ivaylo Sirakov

    2016-11-01

    Full Text Available One of the main challenges in aquaponics is disease control. One possible solution for this is biological control with organisms exerting inhibitory effects on fish and plant pathogens. The aim of this study was to examine the potential of isolating microorganisms that exert an inhibitory effect on both plant and fish pathogens from an established aquaponic system. We obtained 924 isolates on selective King’s B agar and 101 isolates on MRS agar from different compartments of a model aquaponic system and tested them for antagonism against the plant pathogen Pythium ultimum and fish pathogen Saprolegnia parasitica. Overall, 42 isolates were able to inhibit both fungi. Although not yet tested in vivo, these findings open new options for the implementation of biological control of diseases in aquaponics, where plants and fish are cultivated in the same water recirculating system.

  4. Behavioral tagging of extinction learning.

    Science.gov (United States)

    de Carvalho Myskiw, Jociane; Benetti, Fernando; Izquierdo, Iván

    2013-01-15

    Extinction of contextual fear in rats is enhanced by exposure to a novel environment at 1-2 h before or 1 h after extinction training. This effect is antagonized by administration of protein synthesis inhibitors anisomycin and rapamycin into the hippocampus, but not into the amygdala, immediately after either novelty or extinction training, as well as by the gene expression blocker 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole administered after novelty training, but not after extinction training. Thus, this effect can be attributed to a mechanism similar to synaptic tagging, through which long-term potentiation can be enhanced by other long-term potentiations or by exposure to a novel environment in a protein synthesis-dependent fashion. Extinction learning produces a tag at the appropriate synapses, whereas novelty learning causes the synthesis of plasticity-related proteins that are captured by the tag, strengthening the synapses that generated this tag.

  5. Fish Allergy

    Science.gov (United States)

    ... Cause Blog Vision Awards Common Allergens Fish Allergy Fish Allergy Learn about fish allergy, how to read ... that you must avoid both. Allergic Reactions to Fish Finned fish can cause severe and potentially life- ...

  6. Behavioural responses to hypoxia provide a non-invasive method for distinguishing between stress coping styles in fish

    DEFF Research Database (Denmark)

    Laursen, Danielle Caroline; Olsén, Hanna L.; Ruiz-Gomez, Maria de Lourdes

    2011-01-01

    . These individual differences may result in suboptimal production and compromised welfare in aquaculture. An approach to overcome these problems could be to sort fish and optimise rearing conditions according to coping style. It has been previously demonstrated that the proactive and reactive coping styles in fish......, these fish provided an opportunity for verifying a method for sorting fish with respect to coping style by exposure to hypoxia. Groups consisting of 24 individually tagged fish, 12 HR and 12 LR were exposed to hypoxia in a two choice system. The system consisted of a “home” tank provided with cover connected...... to a second brightly “illuminated” tank via a closable doorway. During the experiment, the doorway between the two tanks was opened and hypoxic conditions were gradually induced in the “home” tank by bubbling with nitrogen. The latency time to move away from hypoxic conditions to normoxic conditions...

  7. FISH-BOL and seafood identification: geographically dispersed case studies reveal systemic market substitution across Canada.

    Science.gov (United States)

    Hanner, Robert; Becker, Sven; Ivanova, Natalia V; Steinke, Dirk

    2011-10-01

    The Fish Barcode of Life campaign involves a broad international collaboration among scientists working to advance the identification of fishes using DNA barcodes. With over 25% of the world's known ichthyofauna currently profiled, forensic identification of seafood products is now feasible and is becoming routine. Driven by growing consumer interest in the food supply, investigative reporters from five different media establishments procured seafood samples (n = 254) from numerous retail establishments located among five Canadian metropolitan areas between 2008 and 2010. The specimens were sent to the Canadian Centre for DNA Barcoding for analysis. By integrating the results from these individual case studies in a summary analysis, we provide a broad perspective on seafood substitution across Canada. Barcodes were recovered from 93% of the samples (n = 236), and identified using the Barcode of Life Data Systems "species identification" engine ( www.barcodinglife.org ). A 99% sequence similarity threshold was employed as a conservative matching criterion for specimen identification to the species level. Comparing these results against the Canadian Food Inspection Agency's "Fish List" a guideline to interpreting "false, misleading or deceptive" names (as per s 27 of the Fish Inspection regulations) demonstrated that 41% of the samples were mislabeled. Most samples were readily identified; however, this was not true in all cases because some samples had no close match. Others were ambiguous due to limited barcode resolution (or imperfect taxonomy) observed within a few closely related species complexes. The latter cases did not significantly impact the results because even the partial resolution achieved was sufficient to demonstrate mislabeling. This work highlights the functional utility of barcoding for the identification of diverse market samples. It also demonstrates how barcoding serves as a bridge linking scientific nomenclature with approved market names

  8. Development of a Natural Rearing System to Improve Supplemental Fish Quality, 1996-1998 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Maynard, Desmond J.

    2001-09-13

    This report covers the 1996-1998 Natural Rearing Enhancement System (NATURES) research for increasing hatchery salmon postrelease survival and producing fish with more wild-like behavior, physiology, and morphology prior to release. Experiments were conducted evaluating automatic subsurface feeders; natural diets; exercise systems; seminatural raceway habitat enriched with cover, structure, and substrate; and predator avoidance conditioning for hatchery salmonids. Automatic subsurface feed delivery systems did not affect chinook salmon depth distribution or vulnerability to avian predators. Live-food diets only marginally improved the ability of chinook salmon to capture prey in stream enclosures. A prototype exercise system that can be retrofitted to raceways was developed, however, initial testing indicated that severe amounts of exercise may increase in culture mortality. Rearing chinook salmon in seminatural raceway habitat with gravel substrate, woody debris structure, and overhead cover improved coloration and postrelease survival without impacting in-culture health or survival. Steelhead fry reared in enriched environments with structure, cover, and point source feeders dominated and outcompeted conventionally reared fish. Exposing chinook salmon to caged predators increased their postrelease survival. Chinook salmon showed an antipredator response to chemical stimuli from injured conspecifics and exhibited acquired predator recognition following exposure to paired predator-prey stimuli. The report also includes the 1997 Natural Rearing System Workshop proceedings.

  9. Scanning Cargo Containers with Tagged Neutrons

    International Nuclear Information System (INIS)

    Viesti, G.; Botosso, C.; Fabris, D.; Lunardon, M.; Moretto, S.; Nebbia, G.; Pesente, S.; Zenoni, A.; Donzella, A.; Perot, B.; Carasco, C.; Bernard, S.; Mariani, A.; Szabo, J.-L.; Sannie, G.; Valkovic, V.; Sudac, D.; Nad, K.; Peerani, P.; Sequeira, V.

    2007-01-01

    A new Tagged Neutron Inspection System (TNIS) able to detect illicit materials such as explosives and narcotics in cargo containers has been developed within the EURopean Illicit TRAfficing Countermeasures Kit (EURITRACK) project. After the R and D phase, the inspection portal has been installed and commissioned at the Rijeka seaport in Croatia, where it has been operated in connection with the existing X-ray scanner for a first two-month demonstration campaign. Results obtained are presented and discussed in this paper

  10. Development of "same side" flavour tagging algorithms for measurements of flavour oscillations and $CP$ violation in the $B^0$ mesons system

    CERN Document Server

    Fazzini, Davide; Khanji, Basem

    In this thesis new developments of $\\textit{Flavour Tagging}$ algorithms for the $LHCb$ experiment are presented. The $\\textit{Flavour Tagging}$ is a very usefull tool which allows to determine the flavour of the reconstructed particles, such as the $B^0$ mesons. A correctly identification of the flavour is fundamental in certain measurements such as time-dependent $CP$ violation asymmetries or the $B^0 \\leftrightarrow \\overline{B}^0$ oscillations. Both these type of measurements are exploited by LHCb experiment in the research of new physics beyond the Standard Model. The new developments achieved in this work concern an optimization of the $\\textit{Same Side Tagger}$ algorithms, using protons and pions correlated in charge with the signal $B^0$ to infer its initial flavour. Then two combinations are implemented: the first is a combination of the $\\textit{SS Pion Tagger}$ ($SS\\pi$) and the $\\textit{SS Proton Tagger}$ ($SSp$) in a unique $\\textit{Same Side}$ ($SS$) tagging algorithm; the second one is the fi...

  11. Flavor Tagging at Tevatron incl. calibration and control

    Energy Technology Data Exchange (ETDEWEB)

    Moulik, T.; /Kansas U.

    2007-01-01

    This report summarizes the flavor tagging techniques developed at the CDF and D0 experiments. Flavor tagging involves identification of the B meson flavor at production, whether its constituent is a quark or an anti-quark. It is crucial for measuring the oscillation frequency of neutral B mesons, both in the B{sup 0} and B{sub S} system. The two experiments have developed their unique approaches to flavor tagging, using neural networks, and likelihood methods to disentangle tracks from b decays from other tracks. This report discusses these techniques and the measurement of B{sup 0} mixing, as a means to calibrate the taggers.

  12. Flavour tagging of $b$ mesons in $pp$ collisions at LHCb

    CERN Multimedia

    Mueller, Vanessa

    2016-01-01

    Flavour tagging, i.e. the inference of the production flavour of reconstructed $b$ hadrons, is essential for precision measurements of decay time-dependent $CP$ violation and of mixing parameters in the the neutral $B$ meson systems. LHC's $pp$ collisions with their high track multiplicities constitute a challenging environment for flavour tagging and demand for new and improved strategies. We present recent progress and new developments in flavour tagging at the LHCb experiment, which will allow for a further improvement of $CP$ violation measurements in decays of $B^0$ and $B_s^0$ mesons.

  13. Navigation system for a mobile robot with a visual sensor using a fish-eye lens

    Science.gov (United States)

    Kurata, Junichi; Grattan, Kenneth T. V.; Uchiyama, Hironobu

    1998-02-01

    Various position sensing and navigation systems have been proposed for the autonomous control of mobile robots. Some of these systems have been installed with an omnidirectional visual sensor system that proved very useful in obtaining information on the environment around the mobile robot for position reckoning. In this article, this type of navigation system is discussed. The sensor is composed of one TV camera with a fish-eye lens, using a reference target on a ceiling and hybrid image processing circuits. The position of the robot, with respect to the floor, is calculated by integrating the information obtained from a visual sensor and a gyroscope mounted in the mobile robot, and the use of a simple algorithm based on PTP control for guidance is discussed. An experimental trial showed that the proposed system was both valid and useful for the navigation of an indoor vehicle.

  14. Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research.

    Directory of Open Access Journals (Sweden)

    Takuya Kobayashi

    Full Text Available BACKGROUND: Genetically encoded tag is a powerful tool for protein research. Various kinds of tags have been developed: fluorescent proteins for live-cell imaging, affinity tags for protein isolation, and epitope tags for immunological detections. One of the major problems concerning the protein tagging is that many constructs with different tags have to be made for different applications, which is time- and resource-consuming. METHODOLOGY/PRINCIPAL FINDINGS: Here we report a novel multifunctional green fluorescent protein (mfGFP tag which was engineered by inserting multiple peptide tags, i.e., octa-histidine (8xHis, streptavidin-binding peptide (SBP, and c-Myc tag, in tandem into a loop of GFP. When fused to various proteins, mfGFP monitored their localization in living cells. Streptavidin agarose column chromatography with the SBP tag successfully isolated the protein complexes in a native form with a high purity. Tandem affinity purification (TAP with 8xHis and SBP tags in mfGFP further purified the protein complexes. mfGFP was clearly detected by c-Myc-specific antibody both in immunofluorescence and immuno-electron microscopy (EM. These findings indicate that mfGFP works well as a multifunctional tag in mammalian cells. The tag insertion was also successful in other fluorescent protein, mCherry. CONCLUSIONS AND SIGNIFICANCE: The multifunctional fluorescent protein tag is a useful tool for a wide variety of protein research, and may have the advantage over other multiple tag systems in its higher expandability and compatibility with existing and future tag technologies.

  15. Establishing nursery estuary otolith geochemical tags for Sea Bass (Dicentrarchus labrax): Is temporal stability estuary dependent?

    Science.gov (United States)

    Ryan, Diarmuid; Wögerbauer, Ciara; Roche, William

    2016-12-01

    The ability to determine connectivity between juveniles in nursery estuaries and adult populations is an important tool for fisheries management. Otoliths of juvenile fish contain geochemical tags, which reflect the variation in estuarine elemental chemistry, and allow discrimination of their natal and/or nursery estuaries. These tags can be used to investigate connectivity patterns between juveniles and adults. However, inter-annual variability of geochemical tags may limit the accuracy of nursery origin determinations. Otolith elemental composition was used to assign a single cohort of 0-group sea bass Dicentrarchus labrax to their nursery estuary thus establishing an initial baseline for stocks in waters around Ireland. Using a standard LDFA model, high classification accuracies to nursery sites (80-88%) were obtained. Temporal stability of otolith geochemical tags was also investigated to assess if annual sampling is required for connectivity studies. Geochemical tag stability was found to be strongly estuary dependent.

  16. Aggregations of bluefish Pomatomus saltatrix (L.) at Mediterranean coastal fish farms: seasonal presence, daily patterns and influence of farming activity

    OpenAIRE

    Arechavala-Lopez, Pablo; Izquierdo-Gomez, David; Uglem, Ingebrigt; Sanchez-Jerez, Pablo

    2014-01-01

    Bluefish, Pomatomus saltatrix (Linnaeus, 1766), is commonly observed close to Mediterranean open-sea fish farms. It usually preys on wild fish that are attracted to farms, but also on farmed fish by biting holes in sea cages net walls. In the current telemetry study, we found that the tagged bluefish stayed close to fish farms during spring and early summer. However, most of the tagged fish disappeared from the farms during autumn, when the sea water temperature dropped. When aggregating at f...

  17. Field and laboratory notes on development of a PIT-tag system for spillways - Research and Development of New Marking and Monitoring Technologies

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project addresses how to expand the current fish-tracking technologies to enable the fisheries community to successfully carry out the actions, research, and...

  18. Fish production and some traits of meat quality in rainbow trout (Oncorhynchus mykiss farmed in different production systems

    Directory of Open Access Journals (Sweden)

    St. Stoyanova

    2016-12-01

    Full Text Available Abstract. The rainbow trout (Oncorhynchus mykiss, an important species in Bulgarian aquaculture, is farmed in different production systems as raceways, net cages etc. The aim of the present study was to evaluate the effect of two different rearing systems on fish production, survival rate, meat chemical, mineral composition and farming economic efficiency of culticated rainbow trout (Oncorhynchus mykiss. The information from a survey on fish production and economic efficiency traits was collected from two national rainbow trout farms using the two commonest fish farming systems: in raceways, Happy Fish Ltd fish farm and in net cages, Forest Group Ltd fish farm. The average individual weight gain (g, total weight gain (kg, feed conversion ratio, production costs of 1 kg fish and the economic efficiency coefficients were determined for the two studied fish farms. The final live weight of rainbow trout in net cages was 0.30 kg vs 0.35 kg in raceways. The average individual weight gain was higher in raceways farming system (0.30 kg than in net cages farm (0.26 kg. The mortality rate of fish in raceways was considerably lower: only 1% as compared to that in net cages (4%. The feed conversion ratio at the end of the experiment showed identical values for trout in both farms 1.01. The meat water content of fish reared in raceways and net cages was 77.46±0.65% and 74.52±0.52% respectively (P≤0.01.The protein content of fish meat was higher in fish farmed in net cages 18.84±0.29% as compared to fish cultivated in raceways 17.60±0.49% (P 0.05. Meat fat content of rainbow trout, reared in the net cage system was also considerably higher (5.26±0.30% than the respective parameter in fish farmed in ponds (3.60±0.15% (P≤0.001. The content of Ca and P was higher in rainbow trouts reared in raceways (138.96±1.12 mg.kg and 2844.32±39.31 mg.kg 1 -1 -1 compared with the values of these parameters of fish in net cages (134.46±1.96 mg.kg and 2690.31±42

  19. A new technique for assessing fish passage survival at hydro power stations

    International Nuclear Information System (INIS)

    Heisey, P.G.; Mathur, D.; D'Allesandro, L.

    1993-01-01

    The HI-Z Turb'N Tag recovery method is presented as a new technique that has been successfully used at ten hydropower stations to determine turbine or spillway passage survival of fish. According to this technique, fish are tagged with the Turb'N Tag, which is pear-shaped, made of inflatable latex, and ca 35 mm long and 13 mm wide. The tag is designed to inflate after passage through the turbine, where it then floats the fish to the surface where it can be easily spotted and netted. One tag is sufficient to retrieve fish less than 18 cm long, while three tags may be needed for fish longer than 30 cm. In tests, fish were recovered in under 10 minutes from the tailrace after being tagged and released into a turbine. The tag allowed over 90% recovery of fish in most tests. The technique had minimal effect on the well-being of both hardy and sensitive species and provided an opportunity to examine recovered fish for injuries and retain them up to 72 h to assess possible delayed effects. The technique overcomes most of the logistical problems associated with conventional methods (netting, radio telemetry, mass mark-recapture) to determine turbine passage survival. The technique can also be used to assess effects of spill and fish bypass structures. 9 refs., 2 figs., 1 tab

  20. Fish welfare: Fish capacity to experience pain

    Directory of Open Access Journals (Sweden)

    Vučinić Marijana

    2009-01-01

    Full Text Available Teleost fish possess similar nociceptive processing systems to those found in terrestrial vertebrates. It means that they react to potential painful stimuli in a similar manner as mammals and birds. However, the welfare of fish has been the focus of less research than that of higher vertebrates. Humans may affect the welfare of fish through fisheries, aquaculture and a number of other activities. There is scientific evidence to support the assumption that fish have the capacity to experience pain because they possess functional nociceptors, endogenous opioids and opioid receptors, brain structures involved in pain processing and pathways leading from nociceptors to higher brain structures. Also, it is well documented that some anaesthetics and analgesics may reduce nociceptive responses in fish. Behavioural indicators in fish such as lip-rubbing and rocking behaviours are the best proof that fish react to potential painful stimuli. This paper is an overview of some scientific evidence on fish capacity to experience pain.

  1. Evaluation of surgical implantation of electronic tags in European eel and effects of different suture materials

    DEFF Research Database (Denmark)

    Thorstad, Eva B.; Økland, Finn; Westerberg, Håkan

    2013-01-01

    Effects of implanting data-storage tags in European eel, Anguilla anguilla, and the suitability of different suture materials (braided permanent silk, permanent monofilament, absorbable and absorbable antibacterial) were examined. The tags consisted of an electronic unit and three floats on a wire....... Antibacterial treatment had no effect on inflammation or healing rates. After 6 months, the tag started to become expelled through the incision in five fish (12%). The internal reaction appeared stronger around the floats, suggesting that the coating material of the floats created a tissue reaction, which...

  2. Challenges in performance of food safety management systems: a case of fish processing companies in Tanzania.

    Science.gov (United States)

    Kussaga, Jamal B; Luning, Pieternel A; Tiisekwa, Bendantunguka P M; Jacxsens, Liesbeth

    2014-04-01

    This study provides insight for food safety (FS) performance in light of the current performance of core FS management system (FSMS) activities and context riskiness of these systems to identify the opportunities for improvement of the FSMS. A FSMS diagnostic instrument was applied to assess the performance levels of FSMS activities regarding context riskiness and FS performance in 14 fish processing companies in Tanzania. Two clusters (cluster I and II) with average FSMS (level 2) operating under moderate-risk context (score 2) were identified. Overall, cluster I had better (score 3) FS performance than cluster II (score 2 to 3). However, a majority of the fish companies need further improvement of their FSMS and reduction of context riskiness to assure good FS performance. The FSMS activity levels could be improved through hygienic design of equipment and facilities, strict raw material control, proper follow-up of critical control point analysis, developing specific sanitation procedures and company-specific sampling design and measuring plans, independent validation of preventive measures, and establishing comprehensive documentation and record-keeping systems. The risk level of the context could be reduced through automation of production processes (such as filleting, packaging, and sanitation) to restrict people's interference, recruitment of permanent high-skilled technological staff, and setting requirements on product use (storage and distribution conditions) on customers. However, such intervention measures for improvement could be taken in phases, starting with less expensive ones (such as sanitation procedures) that can be implemented in the short term to more expensive interventions (setting up assurance activities) to be adopted in the long term. These measures are essential for fish processing companies to move toward FSMS that are more effective.

  3. Editorial Tag Endogeneity for News Websites

    OpenAIRE

    Bruno Ribeiro; Ricardo Morla; Amílcar Correia

    2013-01-01

    Editors and journalists at some news websites label their articles with structure and content-related editorial tags. Each article can have more than one tag and each tag can be used in more than one article. A network of tags can be defined whose edges are all possible pairs of tags in each article. Because editorial tags relate to structure and content rather than individual articles, the analysis of a network of editorial tags could assist editorial decisions to prioritize types of content...

  4. A synthesis of tagging studies examining the behaviour and survival of anadromous salmonids in marine environments.

    Directory of Open Access Journals (Sweden)

    S Matthew Drenner

    Full Text Available This paper synthesizes tagging studies to highlight the current state of knowledge concerning the behaviour and survival of anadromous salmonids in the marine environment. Scientific literature was reviewed to quantify the number and type of studies that have investigated behaviour and survival of anadromous forms of Pacific salmon (Oncorhynchus spp., Atlantic salmon (Salmo salar, brown trout (Salmo trutta, steelhead (Oncorhynchus mykiss, and cutthroat trout (Oncorhynchus clarkii. We examined three categories of tags including electronic (e.g. acoustic, radio, archival, passive (e.g. external marks, Carlin, coded wire, passive integrated transponder [PIT], and biological (e.g. otolith, genetic, scale, parasites. Based on 207 papers, survival rates and behaviour in marine environments were found to be extremely variable spatially and temporally, with some of the most influential factors being temperature, population, physiological state, and fish size. Salmonids at all life stages were consistently found to swim at an average speed of approximately one body length per second, which likely corresponds with the speed at which transport costs are minimal. We found that there is relatively little research conducted on open-ocean migrating salmonids, and some species (e.g. masu [O. masou] and amago [O. rhodurus] are underrepresented in the literature. The most common forms of tagging used across life stages were various forms of external tags, coded wire tags, and acoustic tags, however, the majority of studies did not measure tagging/handling effects on the fish, tag loss/failure, or tag detection probabilities when estimating survival. Through the interdisciplinary application of existing and novel technologies, future research examining the behaviour and survival of anadromous salmonids could incorporate important drivers such as oceanography, tagging/handling effects, predation, and physiology.

  5. Electronic tags and genetics explore variation in migrating steelhead kelts (oncorhynchus mykiss), Ninilchik river, Alaska

    Science.gov (United States)

    Nielsen, J.L.; Turner, S.M.; Zimmerman, C.E.

    2011-01-01

    Acoustic and archival tags examined freshwater and marine migrations of postspawn steelhead kelts (Oncorhynchus mykiss) in the Ninilchik River, Alaska, USA. Postspawn steelhead were captured at a weir in 2002-2005. Scale analysis indicated multiple migratory life histories and spawning behaviors. Acoustic tags were implanted in 99 kelts (2002-2003), and an array of acoustic receivers calculated the average speed of outmigration, timing of saltwater entry, and duration of residency in the vicinity of the river mouth. Ocean migration data were recovered from two archival tags implanted in kelts in 2004 (one male and one female). Archival tags documented seasonal differences in maximum depth and behavior with both fish spending 97% of time at sea <6 m depth (day and night). All study fish were double tagged with passive integrated transponder (PIT) tags implanted in the body cavity. Less than 4% of PIT tags were retained in postspawn steelhead. Molecular genetics demonstrated no significant differences in genetic population structure across years or among spawning life history types, suggesting a genetically panmictic population with highly diverse life history characteristics in the Ninilchik River.

  6. Effects of feed loading on nitrogen balances and fish performance in replicated recirculating aquaculture systems

    DEFF Research Database (Denmark)

    Pedersen, Lars-Flemming; Suhr, Karin Isabel; Dalsgaard, Anne Johanne Tang

    2012-01-01

    This study investigated the effects of applying four fixed feed loadings to three replicated recirculating aquaculture systems (RAS) on water quality changes, nitrogenous balances and growth performance of rainbow trout (Oncorhynchus mykiss).Feed loadings ranged from 1.6 to 6.3kgfeed/m3 make-up...... water, with a constant make-up water renewal of 4.7% of total water volume per day in all twelve RAS. Fish densities ranged from 14 to 92kg/m3 during the prolonged trial of 10weeks. Selected water quality parameters were measured during two intensive sampling campaigns, evaluating biofilter...

  7. Folksonomia: a linguagem das tags

    Directory of Open Access Journals (Sweden)

    Juliana de Assis

    2013-01-01

    Full Text Available A radicalização do potencial colaborativo da web atual aponta uma tendência de personalização da recuperação da informação através de ferramentas que exploram a linguagem natural na representação e no compartilhamento de conteúdos ao longo das redes sociais. Tal configuração sócio-técnica traz desafios aos profissionais da informação tanto para a descrição e compreensão dos fenômenos informacionais que ocorrem neste âmbito, quanto para a elaboração de produtos e serviços voltados para um usuário que se apresenta cada vez mais como sujeito informacional ao assumir um papel ativo diante da complexidade que caracteriza a organização da informação em contextos digitais. Este artigo apresenta conclusões de pesquisa, relacionadas às analises da linguagem utilizada em três ambientes colaborativos que utilizam a folksonomia (Social Tagging Systems. A partir de uma perspectiva fundamentada na Semiótica e na Análise de Redes Sociais, são identificadas e descritas as principais manifestações da linguagem gerada e compartilhada pelas redes sociais através destes ambientes.

  8. Tempting To Tag: An Experimental Comparison Of Four Tagging Input Mechanisms

    Directory of Open Access Journals (Sweden)

    Mark Melenhorst

    2010-01-01

    Full Text Available Tagging helps achieve improved indexing and recommendation of resources (e.g., videos or pictures in large data collections. In order to reap the benefits of tagging, people must be persuaded to label the resources they consume. This paper reports on a study in which four different tagging input mechanisms and their effect on users' motivation to tag were compared. The mechanisms consisted of a standard tag input box, a chatbot-like environment, a bookmarking mechanism, and a "tag and vote" game. The results of our experiment show that the use of the nonstandard tagging input mechanisms does not affect users' motivation to tag. In some instances tagging mechanisms were found to distract users from their primary task: consuming resources. Persuading people to tag might be accomplished more effectively by using other motivating tagging mechanisms (e.g., tagging games, or motivation could be created by explaining the usefulness of tagging.

  9. Are fish immune systems really affected by parasites? an immunoecological study of common carp (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Flajšhans Martin

    2011-06-01

    Full Text Available Abstract Background The basic function of the immune system is to protect an organism against infection in order to minimize the fitness costs of being infected. According to life-history theory, energy resources are in a trade-off between the costly demands of immunity and other physiological demands. Concerning fish, both physiology and immunity are influenced by seasonal changes (i.e. temporal variation associated to the changes of abiotic factors (such as primarily water temperature and interactions with pathogens and parasites. In this study, we investigated the potential associations between the physiology and immunocompetence of common carp (Cyprinus carpio collected during five different periods of a given year. Our sampling included the periods with temporal variability and thus, it presented a different level in exposure to parasites. We analyzed which of two factors, seasonality or parasitism, had the strongest impact on changes in fish physiology and immunity. Results We found that seasonal changes play a key role in affecting the analyzed measurements of physiology, immunity and parasitism. The correlation analysis revealed the relationships between the measures of overall host physiology, immunity and parasite load when temporal variability effect was removed. When analyzing separately parasite groups with different life-strategies, we found that fish with a worse condition status were infected more by monogeneans, representing the most abundant parasite group. The high infection by cestodes seems to activate the phagocytes. A weak relationship was found between spleen size and abundance of trematodes when taking into account seasonal changes. Conclusions Even if no direct trade-off between the measures of host immunity and physiology was confirmed when taking into account the seasonality, it seems that seasonal variability affects host immunity and physiology through energy allocation in a trade-off between life important

  10. Bifurcations and Periodic Solutions for an Algae-Fish Semicontinuous System

    Directory of Open Access Journals (Sweden)

    Chuanjun Dai

    2013-01-01

    Full Text Available We propose an algae-fish semicontinuous system for the Zeya Reservoir to study the control of algae, including biological and chemical controls. The bifurcation and periodic solutions of the system were studied using a Poincaré map and a geometric method. The existence of order-1 periodic solution of the system is discussed. Based on previous analysis, we investigated the change in the location of the order-1 periodic solution with variable parameters and we described the transcritical bifurcation of the system. Finally, we provided a series of numerical results to illustrate the feasibility of the theoretical results. These results may help to facilitate a better understanding of algal control in the Zeya Reservoir.

  11. Fish protection at water intakes using a new signal development process and sound system

    International Nuclear Information System (INIS)

    Loeffelman, P.H.; Klinect, D.A.; Van Hassel, J.H.

    1991-01-01

    American Electric Power Company, Inc., is exploring the feasibility of using a patented signal development process and sound system to guide aquatic animals with underwater sound. Sounds from animals such as chinook salmon, steelhead trout, striped bass, freshwater drum, largemouth bass, and gizzard shad can be used to synthesize a new signal to stimulate the animal in the most sensitive portion of its hearing range. AEP's field tests during its research demonstrate that adult chinook salmon, steelhead trout and warmwater fish, and steelhead trout and chinook salmon smolts can be repelled with a properly-tuned system. The signal development process and sound system is designed to be transportable and use animals at the site to incorporate site-specific factors known to affect underwater sound, e.g., bottom shape and type, water current, and temperature. This paper reports that, because the overall goal of this research was to determine the feasibility of using sound to divert fish, it was essential that the approach use a signal development process which could be customized to animals and site conditions at any hydropower plant site

  12. Effects of tag and suture type on survival and growth of brown trout with surgically implanted telemetry tags in the wild

    DEFF Research Database (Denmark)

    Jepsen, Niels; Mikkelsen, Jørgen Skole; Koed, Anders

    2008-01-01

    To test the effects of surgical implants with or without external antennae, 188 wild brown trout Salmo trutta, 150 - 290 mm, were tagged and released in a small river in May 2005. After 5 months, 103 of the fish were recaptured and examined. Thus, information on the relative survival, growth and ...

  13. Performance of Encounternet Tags: Field Tests of Miniaturized Proximity Loggers for Use on Small Birds.

    Directory of Open Access Journals (Sweden)

    Iris I Levin

    Full Text Available Proximity logging is a new tool for understanding social behavior as it allows for accurate quantification of social networks. We report results from field calibration and deployment tests of miniaturized proximity tags (Encounternet, digital transceivers that log encounters between tagged individuals. We examined radio signal behavior in relation to tag attachment (tag, tag on bird, tag on saline-filled balloon to understand how radio signal strength is affected by the tag mounting technique used for calibration tests. We investigated inter-tag and inter-receiver station variability, and in each calibration test we accounted for the effects of antennae orientation. Additionally, we used data from a live deployment on breeding barn swallows (Hirundo rustica erythrogaster to analyze the quality of the logs, including reciprocal agreement in dyadic logs. We evaluated the impact (in terms of mass changes of tag attachment on the birds. We were able to statistically distinguish between RSSI values associated with different close-proximity (<5 m tag-tag distances regardless of antennae orientation. Inter-tag variability was low, but we did find significant inter-receiver station variability. Reciprocal agreement of dyadic logs was high and social networks were constructed from proximity tag logs based on two different RSSI thresholds. There was no evidence of significant mass loss in the time birds were wearing tags. We conclude that proximity loggers are accurate and effective for quantifying social behavior. However, because RSSI and distance cannot be perfectly resolved, data from proximity loggers are most appropriate for comparing networks based on specific RSSI thresholds. The Encounternet system is flexible and customizable, and tags are now light enough for use on small animals (<50 g.

  14. Survival and growth of fish (Lates calcarifer under integrated mangrove-aquaculture and open-aquaculture systems

    Directory of Open Access Journals (Sweden)

    Shanmugaarasu Venkatachalam

    2018-02-01

    Full Text Available The potential use of mangrove swamp for fish farming industry is not clearly known. Therefore, current study was conducted to assess the growth performance of the Asian Seabass, Lates calcarifer cultivated in integrated mangrove-aquaculture system (IMAS and open aquaculture system without mangroves (OAS. Fish survival and biomass production were higher by 11% and 12.5% respectively in the IMAS than those in the OAS. The fish growth performance was higher in monsoon than that in other seasons. It was in association with water quality parameters such as, high levels of DO, chlorophylls-a,b, nitrate-N, DOC, TOC; low levels of light intensity, temperature (air, water, SPM, chlorophyll-c, nitrite-N, ammonia, total phosphate, reactive silicate, and POC; as well with moderate salinity. The water quality seemed to be favourable for growth and survival of the fish. Therefore, integrating the mangroves with fish farming of the Asian seabass is beneficial for better fish survival and biomass production.

  15. An automatic system to search, acquire, and analyse chromosomal aberrations obtained using FISH technique

    International Nuclear Information System (INIS)

    Esposito, R.D.

    2003-01-01

    Full text: Chromosomal aberrations (CA) analysis in peripheral blood lymphocytes is useful both in prenatal diagnoses and cancer cytogenetics, as well as in toxicology to determine the biologically significant dose of specific, both physical and chemical, genotoxic agents to which an individual is exposed. A useful cytogenetic technique for CAs analysis is Fluorescence-in-situ-Hybridization (FISH) which simplifies the automatic Identification and characterisation of aberrations, allowing the visualisation of chromosomes as bright signals on a dark background, and a fast analysis of stable aberrations, which are particularly interesting for late effects. The main limitation of CA analysis is the rarity with which these events occur, and therefore the time necessary to single out a statistically significant number of aberrant cells. In order to address this problem, a prototype system, capable of automatically searching, acquiring, and recognising chromosomal images of samples prepared using FISH, has been developed. The system is able to score large number of samples in a reasonable time using predefined search criteria. The system is based on the appropriately implemented and characterised automatic metaphase finder Metafer4 (MetaSystems), coupled with a specific module for the acquisition of high magnification metaphase images with any combination of fluorescence filters. These images are then analysed and classified using our software. The prototype is currently capable of separating normal metaphase images from presumed aberrant ones. This system is currently in use in our laboratories both by ourselves and by other researchers not involved in its development, in order to carry out analyses of CAs induced by ionising radiation. The prototype allows simple acquisition and management of large quantities of images and makes it possible to carry out methodological studies -such as the comparison of results obtained by different operators- as well as increasing the

  16. A 3D approximate maximum likelihood solver for localization of fish implanted with acoustic transmitters

    Science.gov (United States)

    Li, Xinya; Deng, Z. Daniel; Sun, Yannan; Martinez, Jayson J.; Fu, Tao; McMichael, Geoffrey A.; Carlson, Thomas J.

    2014-11-01

    Better understanding of fish behavior is vital for recovery of many endangered species including salmon. The Juvenile Salmon Acoustic Telemetry System (JSATS) was developed to observe the out-migratory behavior of juvenile salmonids tagged by surgical implantation of acoustic micro-transmitters and to estimate the survival when passing through dams on the Snake and Columbia Rivers. A robust three-dimensional solver was needed to accurately and efficiently estimate the time sequence of locations of fish tagged with JSATS acoustic transmitters, to describe in sufficient detail the information needed to assess the function of dam-passage design alternatives. An approximate maximum likelihood solver was developed using measurements of time difference of arrival from all hydrophones in receiving arrays on which a transmission was detected. Field experiments demonstrated that the developed solver performed significantly better in tracking efficiency and accuracy than other solvers described in the literature.

  17. Reproduction of Persian Gulf anemone fish (Amphiprion clarkii) in captive system

    OpenAIRE

    Javad Sahandi

    2011-01-01

    The present study was carried out to assess the reproduction of Persian Gulf anemone fish,Amphiprion clarkii (Bennett, 1830), in captive conditions with artificial features. Persian Gulf, havinggood relation with Indian Ocean, is one of the important niches of fishes and the specific position ofthis Gulf makes its fishes popular. The yellow tail clown fish which originates to this gulf has the bestsurvival rate and health than the other areas. Live food is the most important factor in product...

  18. Tagging, Encoding, and Jones Optimality

    DEFF Research Database (Denmark)

    Danvy, Olivier; Lopez, Pablo E. Martinez

    2003-01-01

    A partial evaluator is said to be Jones-optimal if the result of specializing a self-interpreter with respect to a source program is textually identical to the source program, modulo renaming. Jones optimality has already been obtained if the self-interpreter is untyped. If the selfinterpreter...... is typed, however, residual programs are cluttered with type tags. To obtain the original source program, these tags must be removed. A number of sophisticated solutions have already been proposed. We observe, however, that with a simple representation shift, ordinary partial evaluation is already Jones......-optimal, modulo an encoding. The representation shift amounts to reading the type tags as constructors for higherorder abstract syntax. We substantiate our observation by considering a typed self-interpreter whose input syntax is higher-order. Specializing this interpreter with respect to a source program yields...

  19. Semi-automatic surface sediment sampling system - A prototype to be implemented in bivalve fishing surveys

    Science.gov (United States)

    Rufino, Marta M.; Baptista, Paulo; Pereira, Fábio; Gaspar, Miguel B.

    2018-01-01

    In the current work we propose a new method to sample surface sediment during bivalve fishing surveys. Fishing institutes all around the word carry out regular surveys with the aim of monitoring the stocks of commercial species. These surveys comprise often more than one hundred of sampling stations and cover large geographical areas. Although superficial sediment grain sizes are among the main drivers of benthic communities and provide crucial information for studies on coastal dynamics, overall there is a strong lack of this type of data, possibly, because traditional surface sediment sampling methods use grabs, that require considerable time and effort to be carried out on regular basis or on large areas. In face of these aspects, we developed an easy and un-expensive method to sample superficial sediments, during bivalve fisheries monitoring surveys, without increasing survey time or human resources. The method was successfully evaluated and validated during a typical bivalve survey carried out on the Northwest coast of Portugal, confirming that it had any interference with the survey objectives. Furthermore, the method was validated by collecting samples using a traditional Van Veen grabs (traditional method), which showed a similar grain size composition to the ones collected by the new method, on the same localities. We recommend that the procedure is implemented on regular bivalve fishing surveys, together with an image analysis system to analyse the collected samples. The new method will provide substantial quantity of data on surface sediment in coastal areas, using a non-expensive and efficient manner, with a high potential application in different fields of research.

  20. Tagging, Encoding, and Jones Optimality

    DEFF Research Database (Denmark)

    Danvy, Olivier; López, Pablo Ernesto Martínes

    2003-01-01

    A partial evaluator is said to be Jones-optimal if the result of specializing a self-interpreter with respect to a source program is textually identical to the source program, modulo renaming. Jones optimality has already been obtained if the self-interpreter is untyped. If the selfinterpreter...... is typed, however, residual programs are cluttered with type tags. To obtain the original source program, these tags must be removed. A number of sophisticated solutions have already been proposed. We observe, however, that with a simple representation shift, ordinary partial evaluation is already Jones...

  1. Tissue-specific tagging of endogenous loci in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Kate Koles

    2016-01-01

    Full Text Available Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners and/or developmental windows of gene expression. To address these limitations, we have developed a method called T-STEP (tissue-specific tagging of endogenous proteins that allows endogenous loci to be tagged in a tissue specific manner. T-STEP uses a combination of efficient CRISPR/Cas9-enhanced gene targeting and tissue-specific recombinase-mediated tag swapping to temporally and spatially label endogenous proteins. We have employed this method to GFP tag OCRL (a phosphoinositide-5-phosphatase in the endocytic pathway and Vps35 (a Parkinson's disease-implicated component of the endosomal retromer complex in diverse Drosophila tissues including neurons, glia, muscles and hemocytes. Selective tagging of endogenous proteins allows, for the first time, cell type-specific live imaging and proteomics in complex tissues.

  2. Wireless SAW passive tag temperature measurement in the collision case

    Science.gov (United States)

    Sorokin, A.; Shepeta, A.; Wattimena, M.

    2018-04-01

    This paper describes temperature measurement in the multisensor systems based on the radio-frequency identification SAW passive tags which are currently applied in the electric power systems and the switchgears. Different approaches of temperature measurement in the collision case are shown here. The study is based on the tag model with specific topology, which allows us to determine temperature through the response signal with time-frequency information. This research considers the collision case for several passive tags as the temperature sensors which are placed in the switchgear. This research proposal is to analyze the possibility of using several SAW passive sensors in the collision case. We consider the using of the different typical elements for passive surface acoustic wave tag which applies as an anticollision passive sensor. These wireless sensors based on the surface acoustic waves tags contain specifically coded structures. This topology makes possible the reliability of increasing tag identification and the temperature measurement in the collision case. As the results for this case we illustrate simultaneous measurement of at least six sensors.

  3. Vertical structure, biomass and topographic association of deep-pelagic fishes in relation to a mid-ocean ridge system

    Science.gov (United States)

    Sutton, T. T.; Porteiro, F. M.; Heino, M.; Byrkjedal, I.; Langhelle, G.; Anderson, C. I. H.; Horne, J.; Søiland, H.; Falkenhaug, T.; Godø, O. R.; Bergstad, O. A.

    2008-01-01

    The assemblage structure and vertical distribution of deep-pelagic fishes relative to a mid-ocean ridge system are described from an acoustic and discrete-depth trawling survey conducted as part of the international Census of Marine Life field project MAR-ECO . The 36-station, zig-zag survey along the northern Mid-Atlantic Ridge (MAR; Iceland to the Azores) covered the full depth range (0 to >3000 m), from the surface to near the bottom, using a combination of gear types to gain a more comprehensive understanding of the pelagic fauna. Abundance per volume of deep-pelagic fishes was highest in the epipelagic zone and within the benthic boundary layer (BBL; 0-200 m above the seafloor). Minimum fish abundance occurred at depths below 2300 m but above the BBL. Biomass per volume of deep-pelagic fishes over the MAR reached a maximum within the BBL, revealing a previously unknown topographic association of a bathypelagic fish assemblage with a mid-ocean ridge system. With the exception of the BBL, biomass per volume reached a water column maximum in the bathypelagic zone between 1500 and 2300 m. This stands in stark contrast to the general "open-ocean" paradigm that biomass decreases exponentially from the surface downwards. As much of the summit of the MAR extends into this depth layer, a likely explanation for this mid-water maximum is ridge association. Multivariate statistical analyses suggest that the dominant component of deep-pelagic fish biomass over the northern MAR was a wide-ranging bathypelagic assemblage that was remarkably consistent along the length of the ridge from Iceland to the Azores. Integrating these results with those of previous studies in oceanic ecosystems, there appears to be adequate evidence to conclude that special hydrodynamic and biotic features of mid-ocean ridge systems cause changes in the ecological structure of deep-pelagic fish assemblages relative to those at the same depths over abyssal plains. Lacking terrigenous input of

  4. WebTag: Web browsing into sensor tags over NFC.

    Science.gov (United States)

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Alvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm.

  5. Tempting to Tag : An Experimental Comparison of Four Tagging Input Mechanisms

    OpenAIRE

    Melenhorst, Mark; van Velsen, Lex

    2010-01-01

    Tagging helps achieve improved indexing and recommendation of resources (e.g., videos or pictures) in large data collections. In order to reap the benefits of tagging, people must be persuaded to label the resources they consume. This paper reports on a study in which four different tagging input mechanisms and their effect on users' motivation to tag were compared. The mechanisms consisted of a standard tag input box, a chatbot-like environment, a bookmarking mechanism, and a "tag and v...

  6. Annual coded wire tag program (Washington) missing production groups: annual report for 1997; ANNUAL

    International Nuclear Information System (INIS)

    Byrne, J.; Fuss, H.; Ashbrook, C.

    1998-01-01

    The Bonneville Power Administration (BPA) funds the ''Annual Coded Wire Tag Program - Missing Production Groups for Columbia River Hatcheries'' project. The Washington Department of Fish and Wildlife (WDFW), Oregon Department of Fish and Wildlife (ODFW) and the United States Fish and Wildlife Service (USFWS) all operate salmon and steelhead rearing programs in the Columbia River basin. The intent of the funding is to coded-wire tag at least one production group of each species at each Columbia Basin hatchery to provide a holistic assessment of survival and catch distribution over time and to meet various measures of the Northwest Power Planning Councils (NWPPC) Fish and Wildlife Program. The WDFW project has three main objectives: (1) coded-wire tag at least one production group of each species at each Columbia Basin hatchery to enable evaluation of survival and catch distribution over time, (2) recover coded-wire tags from the snouts of fish tagged under objective 1 and estimate survival, contribution, and stray rates for each group, and (3) report the findings under objective 2 for all broods of chinook, and coho released from WDFW Columbia Basin hatcheries. Objective 1 for FY-97 was met with few modifications to the original FY-97 proposal. Under Objective 2, snouts containing coded-wire tags that were recovered during FY-97 were decoded. Under Objective 3, survival, contribution and stray rate estimates for the 1991-96 broods of chinook and 1993-96 broods of coho have not been made because recovery data for 1996-97 fisheries and escapement are preliminary. This report summarizes recovery information through 1995

  7. SUSTAINABLE TILAPIA PRODUCTION IN A BACKYARD SYSTEM FOR RURAL AREAS USING FISH BY-PRODUCTS IN FORMULATED DIETS

    Directory of Open Access Journals (Sweden)

    Servando Rueda Lopez

    2013-12-01

    Full Text Available The aim of the present study was to demonstrate the feasibility of producing Tilapia (Oreochromis niloticuss, L. with formulated diets containing acid fish silage made with Hancock (Pterigoplichthys multiradiatus by-products in substitution of fishmeal. Thus four treatments containing increasing levels (0, 5, 10 and 15% of acid fish silage to substitute fishmeal were used to feed tilapia juveniles in triplicate groups (average 1.5±0.7 g and 4.3±1.0 cm.  Fish silage was prepared by adding citric and phosphoric acids at 2.6% each resulting in a liquefaction of the chop by-product mixture. After two weeks, the diets were prepared to contain 32-35% protein content. After 50 days of experimental period a total growth increase up to 827% was observed without significant differences among treatments. A specific growth rate from 3.70 to 4.10 and a survival rate from 96 to 99% was observed.  The cost analysis shows a saving on production cost up to 23.87% using the highest incorporation level of fish silage. The use of Aquaculture Recirculation System made with components normally found in a hardware store in rural or semi urban areas is discussed. It is concluded that fish offal shows a promising activity that favors the increase in fish protein production to resolve the demand of cheap protein.

  8. Fish oil intake induces UCP1 upregulation in brown and white adipose tissue via the sympathetic nervous system.

    Science.gov (United States)

    Kim, Minji; Goto, Tsuyoshi; Yu, Rina; Uchida, Kunitoshi; Tominaga, Makoto; Kano, Yuriko; Takahashi, Nobuyuki; Kawada, Teruo

    2015-12-17

    Brown adipose tissue (BAT) plays a central role in regulating energy homeostasis, and may provide novel strategies for the treatment of human obesity. BAT-mediated thermogenesis is regulated by mitochondrial uncoupling protein 1 (UCP1) in classical brown and ectopic beige adipocytes, and is controlled by sympathetic nervous system (SNS). Previous work indicated that fish oil intake reduces fat accumulation and induces UCP1 expression in BAT; however, the detailed mechanism of this effect remains unclear. In this study, we investigated the effect of fish oil on energy expenditure and the SNS. Fish oil intake increased oxygen consumption and rectal temperature, with concomitant upregulation of UCP1 and the β3 adrenergic receptor (β3AR), two markers of beige adipocytes, in the interscapular BAT and inguinal white adipose tissue (WAT). Additionally, fish oil intake increased the elimination of urinary catecholamines and the noradrenaline (NA) turnover rate in interscapular BAT and inguinal WAT. Furthermore, the effects of fish oil on SNS-mediated energy expenditure were abolished in transient receptor potential vanilloid 1 (TRPV1) knockout mice. In conclusion, fish oil intake can induce UCP1 expression in classical brown and beige adipocytes via the SNS, thereby attenuating fat accumulation and ameliorating lipid metabolism.

  9. A step towards seascape scale conservation: using vessel monitoring systems (VMS to map fishing activity.

    Directory of Open Access Journals (Sweden)

    Matthew J Witt

    Full Text Available BACKGROUND: Conservation of marine ecosystems will require a holistic understanding of fisheries with concurrent spatial patterns of biodiversity. METHODOLOGY/PRINCIPAL FINDINGS: Using data from the UK Government Vessel Monitoring System (VMS deployed on UK-registered large fishing vessels we investigate patterns of fisheries activity on annual and seasonal scales. Analysis of VMS data shows that regions of the UK European continental shelf (i.e. Western Channel and Celtic Sea, Northern North Sea and the Goban Spur receive consistently greater fisheries pressure than the rest of the UK continental shelf fishing zone. CONCLUSIONS/SIGNIFICANCE: VMS provides a unique and independent method from which to derive patterns of spatially and temporally explicit fisheries activity. Such information may feed into ecosystem management plans seeking to achieve sustainable fisheries while minimising putative risk to non-target species (e.g. cetaceans, seabirds and elasmobranchs and habitats of conservation concern. With multilateral collaboration VMS technologies may offer an important solution to quantifying and managing ecosystem disturbance, particularly on the high-seas.

  10. Chemical and visual sensory systems in feeding behaviour of the Antarctic fish Ophthalmolycus amberensis (Zoarcidae

    Directory of Open Access Journals (Sweden)

    Edith Fanta

    2001-03-01

    Full Text Available The Antarctic eelpout Ophthalmolycus amberensis occurs in Admiralty Bay (King George Island, South Shetlands, at 140-200m depth, where light intensity is low. To assess behavioural and sensory adaptations for feeding under these conditions, laboratory tests were undertaken. Dead krill, fish fillet, and live amphipods were the preferred food items. Feeding responses were mainly induced by chemical stimuli. Visual stimuli were weak elicitors, leading to a long delay in the initiation of feeding behaviour. These fishes present a large olfactory epithelium, a high density of taste buds on the snout and close to the nostrils, and a retina that contained long rods, but no cones. Food selection was observed. Varied types of taste buds were present on the lips and in the oro-pharyngeal cavity. The capacity to use a chemo-sensory system as first elicitor for food detection, either in the absence or presence of light, allows O. amberensis to efficiently exploit different habitats at the sea bottom, in all Antarctic seasons.

  11. Peracetic acid is a suitable disinfectant for recirculating fish-microalgae integrated multi-trophic aquaculture systems

    Directory of Open Access Journals (Sweden)

    Dibo Liu

    2016-11-01

    Full Text Available Integrated multi-trophic aquaculture (IMTA is a promising direction for the sustainable development of aquaculture. Microalgae have good potential to be integrated with recirculating aquaculture systems because they can use the nitrogen excreted from fish and share the same optimal pH value as in aquaculture. As a byproduct, the microalgae biomass can be used for fish feed or biofuel. However, the recirculating fish-microalgae IMTA system is under constant threat from fish pathogens and phytoplankton-lytic bacteria. Therefore, it is necessary to apply proper disinfectants as prophylaxis or treatment which are effective against these threats, but safe to fish and microalgae. For this purpose, peracetic acid (PAA is a valid option because it is highly effective against fish pathogens and bacteria at low concentrations and degrades spontaneously to harmless residues. In the present study, we exposed the culture of a marine microalgae Tetraselmis chuii once per day for four days to four PAA products with differing hydrogen peroxide (H2O2/PAA proportions at two concentrations (1 and 2 mg L−1 PAA. The H2O2 solutions at equivalent total peroxide (H2O2 + PAA concentrations were tested in parallel. The results show that the growth and photosynthesis of T. chuii were not affected by three of the PAA products (Wofasteril® E400, Wofasteril® E250 and Applichem® 150 and equivalent H2O2 solutions at both concentrations. In contrast, Wofasteril® Lspez and an equivalent H2O2 solution at both concentrations caused irreversible culture collapse, photosynthesis dysfunction and irreversible cell damage. In conclusion, PAA products with low proportions of H2O2 are optimal disinfectants for fish-microalgae IMTA systems.

  12. Folksonomia: a linguagem das tags

    Directory of Open Access Journals (Sweden)

    Juliana de Assis

    2013-04-01

    Full Text Available http://dx.doi.org/10.5007/1518-2924.2013v18n36p85   A radicalização do potencial colaborativo da web atual aponta uma tendência de personalização da recuperação da informação através de ferramentas que exploram a linguagem natural na representação e no compartilhamento de conteúdos ao longo das redes sociais. Tal configuração sócio-técnica traz desafios aos profissionais da informação tanto para a descrição e compreensão dos fenômenos informacionais que ocorrem neste âmbito, quanto para a elaboração de produtos e serviços voltados para um usuário que se apresenta cada vez mais como sujeito informacional ao assumir um papel ativo diante da complexidade que caracteriza a organização da informação em contextos digitais. Este artigo apresenta conclusões de pesquisa, relacionadas às analises da linguagem utilizada em três ambientes colaborativos que utilizam a folksonomia (Social Tagging Systems. A partir de uma perspectiva fundamentada na Semiótica e na Análise de Redes Sociais, são identificadas e descritas as principais manifestações da linguagem gerada e compartilhada pelas redes sociais através destes ambientes.

  13. Immunity to Fish Rhabdoviruses

    OpenAIRE

    Purcell, Maureen K.; Laing, Kerry J.; Winton, James R.

    2012-01-01

    Members of the family Rhabdoviridae are single-stranded RNA viruses and globally important pathogens of wild and cultured fish and thus relatively well studied in their respective hosts or other model systems. Here, we review the protective immune mechanisms that fish mount in response to rhabdovirus infections. Teleost fish possess the principal components of innate and adaptive immunity found in other vertebrates. Neutralizing antibodies are critical for long-term protection from fish rhabd...

  14. Development and Validation of a Biodynamic Model for Mechanistically Predicting Metal Accumulation in Fish-Parasite Systems.

    Directory of Open Access Journals (Sweden)

    T T Yen Le

    Full Text Available Because of different reported effects of parasitism on the accumulation of metals in fish, it is important to consider parasites while interpreting bioaccumulation data from biomonitoring programmes. Accordingly, the first step is to take parasitism into consideration when simulating metal bioaccumulation in the fish host under laboratory conditions. In the present study, the accumulation of metals in fish-parasite systems was simulated by a one-compartment toxicokinetic model and compared to uninfected conspecifics. As such, metal accumulation in fish was assumed to result from a balance of different uptake and loss processes depending on the infection status. The uptake by parasites was considered an efflux from the fish host, similar to elimination. Physiological rate constants for the uninfected fish were parameterised based on the covalent index and the species weight while the parameterisation for the infected fish was carried out based on the reported effects of parasites on the uptake kinetics of the fish host. The model was then validated for the system of the chub Squalius cephalus and the acanthocephalan Pomphorhynchus tereticollis following 36-day exposure to waterborne Pb. The dissolved concentration of Pb in the exposure tank water fluctuated during the exposure, ranging from 40 to 120 μg/L. Generally, the present study shows that the one-compartment model can be an effective method for simulating the accumulation of metals in fish, taking into account effects of parasitism. In particular, the predicted concentrations of Cu, Fe, Zn, and Pb in the uninfected chub as well as in the infected chub and the acanthocephalans were within one order of magnitude of the measurements. The variation in the absorption efficiency and the elimination rate constant of the uninfected chub resulted in variations of about one order of magnitude in the predicted concentrations of Pb. Inclusion of further assumptions for simulating metal accumulation

  15. Spatial and seasonal variations of fish assemblages in mangrove creek systems in Zanzibar (Tanzania)

    Science.gov (United States)

    Mwandya, Augustine W.; Gullström, Martin; Andersson, Mathias H.; Öhman, Marcus C.; Mgaya, Yunus D.; Bryceson, Ian

    2010-11-01

    Spatial and seasonal variations of fish assemblage composition were studied in three non-estuarine mangrove creeks of Zanzibar (Tanzania). Fish were collected monthly for one year at three sites (lower, intermediate and upper reaches) in each creek using a seine net (each haul covering 170 m 2). Density, species number and diversity of fish were all higher at sites with dense cover of macrophytes (seagrass and macroalgae) than over unvegetated sandy sites. In general, fish assemblages mainly comprised juveniles of a few abundant taxa, e.g. Mugil cephalus, Mugilidae spp. and Leiognathus equulus at sites with mud substratum and Gerres oyena, Lethrinus harak and Sillago sihama at sites dominated by macrophytes. Multivariate analyses revealed significant separations in fish assemblage composition within the two creeks where the bottom substratum differed among sites. Overall, season seemed to have little effect on density, species number, diversity index ( H') and assemblage structure of fish. Water condition variables were also relatively stable across the season, although a short-term fluctuation primarily induced by decreased salinity, occurred during the heavy rains in April and May. Fish assemblage structure was not significantly affected by any of the abiotic factors tested. However, significant regressions were found between the other fish variables and environmental variables, but since these associations were mostly species-specific and generally inconsistent, we suggest that the overall distribution patterns of fish were mainly an effect of particular substrate preferences of fish species rather than contemporary water conditions.

  16. Phylogeny and FISH probe analysis of the “Candidatus Competibacter”-lineage in wastewater treatment systems

    DEFF Research Database (Denmark)

    Nittami, Tadashi; McIlroy, Simon Jon; Kanai, Eri

    Our understanding of the microbial ecology of enhanced biological phosphorus removal (EBPR) wastewater treatment systems has been greatly advanced through the application of molecular methods such as fluorescence in situ hybridization (FISH). Considerable attention has been directed at the identi......Our understanding of the microbial ecology of enhanced biological phosphorus removal (EBPR) wastewater treatment systems has been greatly advanced through the application of molecular methods such as fluorescence in situ hybridization (FISH). Considerable attention has been directed...... at the identification and characterization of the glycogen accumulating organisms (GAO), a phenotypic group thought to compete with the polyphosphate accumulating organisms (PAO) for resources at the theoretical expense of EBPR efficiency. Demonstrated candidates for members of the GAO phenotype include...... the gammaproteobacterial “Candidatus Competibacter”-lineage. The group is currently delineated by 8 FISH probe defined phylotypes, although further undescribed phylogenetic diversity beyond what is covered by these probes is evident. Where studied, marked differences in physiology between members are observed, including...

  17. Satellite Tags- Guam/CNMI EEZ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Satellite tagging was implemented in 2013. Satellite tagging is conducted using a Dan Inject air rifle and deployment arrows designed by Wildlife Computers. Two...

  18. HMSRP Hawaiian Monk Seal Tag Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records for all tags applied to Hawaiian monk seals since 1981. These tags were applied by PSD personnel and cooperating scientists as part of...

  19. Cellular and circuit properties supporting different sensory coding strategies in electric fish and other systems.

    Science.gov (United States)

    Marsat, Gary; Longtin, André; Maler, Leonard

    2012-08-01

    Neural codes often seem tailored to the type of information they must carry. Here we contrast the encoding strategies for two different communication signals in electric fish and describe the underlying cellular and network properties that implement them. We compare an aggressive signal that needs to be quickly detected, to a courtship signal whose quality needs to be evaluated. The aggressive signal is encoded by synchronized bursts and a predictive feedback input is crucial in separating background noise from the communication signal. The courtship signal is accurately encoded through a heterogenous population response allowing the discrimination of signal differences. Most importantly we show that the same strategies are used in other systems arguing that they evolved similar solutions because they faced similar tasks. Copyright © 2012 Elsevier Ltd. All rights reserved.

  20. Ingestion and selection of suprabenthic crustaceans by small-sized fishes in a lower saltmarsh system

    Directory of Open Access Journals (Sweden)

    Yoko Wakabara

    1996-01-01

    Full Text Available This study was performed in the lower saltmarsh system of the Arrozal, in the Cananéia lagoon estuarine region (25º02'S - 47º56'W, Brazil. Suprabenthic fauna was surveyed Wlth a small sledge and fishes were captured with casting and set nets to analyse: crustacean fauna as food for local fish species; difference in the diet at different times of the year; if there is diet overlap between species and the feeding behaviour of the species analysed. The fauna of Arrozal is poor in species, dominated mainly by Metamysidopsis alongata atlantica, Acartia lilljeborgi, Atylus minikoi, decapod larvae, and reveals a strong seasonal variation. The fishes were ali camivorous with suprabenthic crustacean as their main food resource. Seasonal changes in food supply are also reflected in the diet. Of the 12 flSh species collected six were opportunistlc feeders whereas six others were selective feeders. Food overlap value of 0.08 for ali of the fish community indicates an almost completely distinct food niches. The increased overlapping of summer food between Cathorops spixii and species of Group 11 and between Oligoplites sp and species of Group I seems to have two different explanations: 1 the mmIDishing of food supply for species feeding on benthic originated suprabenthic crustaceans and 2 overabundance of planktonic forms of suprabenthos as well as a period of high feeding activity of fishes with such diet.O presente estudo foi realizado no infralitoral contíguo à marisma, na Ponta do Arrozal, região estuarina lagunar de Cananéia (25º02'S - 47º56'W, Brasil. A fauna suprabêntica foi amostrada com uma pequena draga e os peixes capturados com tarrafa e rede de espera, com a finalidade de analisar: a composição de espécies dos crustáceos suprabênticos como itens alimentares dos peixes; diferenças na dieta em diferentes época do ano; se ocorre sobreposição alimentar entre as espécies e o comportamento alimentar: das espécies de peixes

  1. Fish passage post-construction issues: analysis of distribution, attraction and passage efficiency metrics at the Baguari Dam fish ladder to approach the problem

    Directory of Open Access Journals (Sweden)

    Luiz Gustavo Martins da Silva

    Full Text Available Fish passages are considered the oldest management tool used to minimize the impact of blocking fish migratory routes by hydroelectric power plants. However, fish passages are being installed without specific criteria in Brazil, with severe consequences to the conservation of the local fish fauna. Therefore, basic data gathered for fish passages already constructed could contribute to define operational rules, in addition to offer subsidies to decision-making and design of future facilities. Thus, the fish ladder of Baguari Dam was evaluated regarding temporal distribution, attraction, and ascension of the local fish fauna. A total of 20 fish samples were conducted immediately downstream of the dam and inside the fish ladder, from January 2010 to June 2011. Seasonal variation in fish abundance and richness was registered below the dam and inside the passage, with higher number of migratory fish in the reproductive season (Kruskall-Wallis, p = 0.04 and p = 0.05. Furthermore, higher concentration of migratory allochthonous and non-migratory species was registered for the spill bay (Wilcoxon, p = 0.009 and p = 0.006 compared to the tailrace, where the fish ladder entrance is located. This result suggests low efficiency of the attraction system of the mechanism during the reproductive period. Once entering the fish ladder, migratory species apparently ascend the facility due to the similar distribution throughout different stretches. Generally, the results showed that an operational rule for the Baguari Dam fish ladder should consider running the facility only during the reproductive period, unless the objectives of the passage are well defined. The attraction system must be more precisely evaluated, using technologies such as radiotelemetry. Similarly, fish ascension also should be better analyzed to evaluate the time spent to ascend and its influence in the reproductive biology of the species using the ladder. Pit-tag system could be used to

  2. First testing of an AUV mission planning and guidance system for water quality monitoring and fish behavior observation in net cage fish farming

    Directory of Open Access Journals (Sweden)

    D. Karimanzira

    2014-12-01

    Full Text Available Recently, underwater vehicles have become low cost, reliable and affordable platforms for performing various underwater tasks. While many aquaculture systems are closed with no harmful output, open net cage fish farms and land-based fish farms can discharge significant amounts of wastewater containing nutrients, chemicals, and pharmaceuticals that impact on the surrounding environment. Although aquaculture development has often occurred outside a regulatory framework, government oversight is increasingly common at both the seafood quality control level, and at baseline initiatives addressing the basic problem of pollution generated by culture operations, e.g. the European marine and maritime directives. This requires regular, sustainable and cost-effective monitoring of the water quality. Such monitoring needs devices to detect the water quality in a large sea area at different depths in real time. This paper presents a concept for a guidance system for a carrier (an autonomous underwater vehicle of such devices for the automated detection and analysis of water quality parameters.

  3. Neuronal nitric oxide synthase in the olfactory system of an adult teleost fish Oreochromis mossambicus.

    Science.gov (United States)

    Singru, Praful S; Sakharkar, Amul J; Subhedar, Nishikant

    2003-07-11

    The aim of the present study is to explore the distribution of nitric oxide synthase in the olfactory system of an adult teleost, Oreochromis mossambicus using neuronal nitric oxide synthase (nNOS) immunocytochemistry and nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) histochemistry methods. Intense nNOS immunoreactivity was noticed in several olfactory receptor neurons (ORNs), in their axonal extensions over the olfactory nerve and in some basal cells of the olfactory epithelium. nNOS containing fascicles of the ORNs enter the bulb from its rostral pole, spread in the olfactory nerve layer in the periphery of the bulb and display massive innervation of the olfactory glomeruli. Unilateral ablation of the olfactory organ resulted in dramatic loss of nNOS immunoreactivity in the olfactory nerve layer of the ipsilateral bulb. In the olfactory bulb of intact fish, some granule cells showed intense immunoreactivity; dendrites arising from the granule cells could be traced to the glomerular layer. Of particular interest is the occurrence of nNOS immunoreactivity in the ganglion cells of the nervus terminalis. nNOS containing fibers were also encountered in the medial olfactory tracts as they extend to the telencephalon. The NADPHd staining generally coincides with that of nNOS suggesting that it may serve as a marker for nNOS in the olfactory system of this fish. However, mismatch was encountered in the case of mitral cells, while all are nNOS-negative, few were NADPHd positive. The present study for the first time revealed the occurrence of nNOS immunoreactivity in the ORNs of an adult vertebrate and suggests a role for nitric oxide in the transduction of odor stimuli, regeneration of olfactory epithelium and processing of olfactory signals.

  4. Fish cognition

    OpenAIRE

    Bshary, Redouan; Brown, Culum

    2017-01-01

    The central nervous system, and the brain in particular, is one of the most remarkable products of evolution. This system allows an individual to acquire, process, store and act on information gathered from the environment. The resulting flexibility in behavior beyond genetically coded strategies is a prime adaptation in animals. The field of animal cognition examines the underlying processes and mechanisms. Fishes are a particularly interesting group of vertebrates to study cognition for two...

  5. Survival of radio-tagged Atlantic salmon ( Salmo salar L.) and trout ( Salmo trutta L.) smolts passing a reservoir during seaward migration

    DEFF Research Database (Denmark)

    Jepsen, Niels; Aarestrup, Kim; Økland, F.

    1998-01-01

    tagged with internal miniature radio-transmitters, and released in the river just upstream the reservoir on May 1, 1996. The salmon smelts were hatchery-reared, while the trout smelts were wild fish, caught in a smelt trap. The tagged smelts were tracked daily for 3 weeks, and when possible the cause...

  6. The Development of Reusable Luggage Tag with the Internet of Things for Mobile Tracking and Environmental Sustainability

    Directory of Open Access Journals (Sweden)

    Eugene Y. C. Wong

    2016-12-01

    Full Text Available With more than two billion passengers worldwide travelling by air each year, vast amounts of lost luggage and disposable paper adhesive luggage tags are pushing the aviation industry to improve luggage tracking and reduce the one-off adhesive luggage paper tags. This paper reviews the current application of Radio Frequency Identification (RFID in the luggage handling system and proposes the Internet of Things’ (IoT development of the reusable luggage tag to facilitate aviation luggage handling, the tracking process and environmental conservation. A framework of IoT and its RFID components for the proposed reusable tag are presented. An integrated cyber-physical system, including a database management system and mobile app, for the reusable luggage tag is developed. Future studies will enhance the methodology of integrating the retail system, luggage tag, airport check-in counter, luggage handling system, aircraft, and the destination airport through the use of the tag, readers, antenna, and mobile devices.

  7. Occurrence, size, and tag retention of sneaker male hatchery rainbow trout

    Science.gov (United States)

    Isely, J.J.; Grabowski, T.B.

    2004-01-01

    One alternative reproductive tactic involving early-maturing, cryptic males is referred to as "sneaking." Although sneakers tend to be easily detectable upon close inspection, little is known about the proportion of a fish population consisting of sneakers. We examined 15,400 age-1 rainbow trout Oncorhynchus mykiss in a hatchery. Total length (mm), wet weight (g), and sex (sneaker male or unknown) were recorded for each fish. We also individually tagged each sneaker male with soft visual implant alphanumeric (VIalpha) tags that were sequentially numbered and held the fish for 25 d before inspection. Sneakers constituted 2.8% of the hatchery rainbow trout population and were smaller in total length and weight than typical rainbow trout of the same age. Retention of the VIalpha tags in sneakers was 58.9%, significantly lower than has been reported under similar circumstances. We found that sneaker males may contribute substantially to hatchery populations. Reduced tag retention in sneakers may bias studies evaluating the effect of hatchery fish on wild populations. We believe that hatchery-produced sneaker males have the potential to contribute importantly to the genetic composition of wild populations.

  8. Movements of adult Atlantic salmon in relation to a hydroelectric dam and fish ladder

    International Nuclear Information System (INIS)

    Gowans, A.R.D.; Priede, I.G.

    1999-01-01

    The movements of adult Atlantic salmon were recorded as they approached, entered and ascended the pool-and-orifice fish ladder at Pitlochry Dam, Scotland. Thirty-nine returning salmon were captured in the River Tummel by rod-and-line angling, radio-tagged and released near where they were caught. The subsequent movements of each fish were then monitored. An electronic fish counter collected additional data on movements of untagged fish past a fixed point in the ladder. Of the 39 fish that were radio-tagged, 29 individuals were recorded approaching and ascending the ladder. The remaining fish either did not approach the dam (three fish), approached the dam after detailed tracking had ended (two fish), were recaptured by anglers (three fish), or the radio tags failed (two fish). Salmon released earlier in the year delayed longer before first approaching the dam. Delays between first approaching the dam and ascent of the ladder were greater for fish that approached the dam earlier. The majority of salmon visited the ladder entrance more than once (maximum 10 visits) before ascending. Having entered, all but four salmon ascended the fish ladder successfully on their first attempt. The four individuals that failed to do so succeeded on their second attempt. The rate at which salmon ascended the ladder was related directly to temperature. The shortest ascent time of a radio-tagged salmon was 5.25 h. Movements of eight of 11 tagged fish through the ladder ceased with the onset of darkness but continued on the following morning. No radio-tagged fish entered the ladder at temperatures below 9 o C. Similarly, few untagged fish were recorded ascending the ladder by the electronic fish counter at water temperatures below 8.5 o C. Records from the fish counter indicated that 92% of upstream movements were made during daylight. (author)

  9. Spatial Structure and Temporal Variation of Fish Communities in the Upper Mississippi River System

    National Research Council Canada - National Science Library

    Chick, John H; Ickes, Brian S; Pegg, Mark A; Barko, Valerie A; Hrabik, Robert A; Herzog, David P

    2005-01-01

    Variation in community composition (presence/absence data) and structure (relative abundance) of Upper Mississippi River fishes was assessed using data from the Long Term Resource Monitoring Program...

  10. Semiquantitative analysis of gaps in microbiological performance of fish processing sector implementing current food safety management systems: a case study.

    Science.gov (United States)

    Onjong, Hillary Adawo; Wangoh, John; Njage, Patrick Murigu Kamau

    2014-08-01

    Fish processing plants still face microbial food safety-related product rejections and the associated economic losses, although they implement legislation, with well-established quality assurance guidelines and standards. We assessed the microbial performance of core control and assurance activities of fish exporting processors to offer suggestions for improvement using a case study. A microbiological assessment scheme was used to systematically analyze microbial counts in six selected critical sampling locations (CSLs). Nine small-, medium- and large-sized companies implementing current food safety management systems (FSMS) were studied. Samples were collected three times on each occasion (n = 324). Microbial indicators representing food safety, plant and personnel hygiene, and overall microbiological performance were analyzed. Microbiological distribution and safety profile levels for the CSLs were calculated. Performance of core control and assurance activities of the FSMS was also diagnosed using an FSMS diagnostic instrument. Final fish products from 67% of the companies were within the legally accepted microbiological limits. Salmonella was absent in all CSLs. Hands or gloves of workers from the majority of companies were highly contaminated with Staphylococcus aureus at levels above the recommended limits. Large-sized companies performed better in Enterobacteriaceae, Escherichia coli, and S. aureus than medium- and small-sized ones in a majority of the CSLs, including receipt of raw fish material, heading and gutting, and the condition of the fish processing tables and facilities before cleaning and sanitation. Fish products of 33% (3 of 9) of the companies and handling surfaces of 22% (2 of 9) of the companies showed high variability in Enterobacteriaceae counts. High variability in total viable counts and Enterobacteriaceae was noted on fish products and handling surfaces. Specific recommendations were made in core control and assurance activities

  11. The Addition of Simple Biological Filters of Different Capacity to Semi‑Recirculating Fish Rearing System and Its Effects

    Directory of Open Access Journals (Sweden)

    Štěpán Lang

    2017-01-01

    Full Text Available Aquaculture is currently one of the fastest growing food-producing sectors, accounting for around 50 % of the world’s food fish production. Limited resources, together with climatic change, have stimulated the search for solutions to support and sustain the production of fish as a source of protein for human consumption. The integration of a biological filtration (BF into a semi-recirculating fish rearing system can increase its carrying capacity and increase system efficiency compared to its’ energy consumption with minimum changes of system composition and minimal costs. Question is the capacity of the BF installed to a system and how it affects water quality. Two different amounts of BF media (surface added to semi-recirculating rearing system compared with the same system without BF were tested in case of this study. The results have shown that if the BF capacity is insufficient, BF can have negative effects to the quality of water environment. The insufficient amount of BF media caused 4 times reduction of ammonia nitrogen (N-NH4+ in system with BF compared to non BF system so it increased the system capacity for feed load 4 times. On the other hand it also increased nitrite nitrogen concentrations permanently more than 5.8 times for BF system compared to non BF system and increased rearing costs because the need of adding chlorides to the system to protect fish from nitrites toxicity. When the BF was dimensioned properly (next year there were almost no N-NH4+ in a system (0.10 mg.l−1 and the concentration of N-NO2− was kept at low levels too (0.150 mg .l−1. The nitrates (N‑NO3− concentration reached the level of 5.37 and 8.65 mg.l−1 in 2012 and 2013 respectively.

  12. In vivo phosphorylation of a peptide tag for protein purification.

    Science.gov (United States)

    Goux, Marine; Fateh, Amina; Defontaine, Alain; Cinier, Mathieu; Tellier, Charles

    2016-05-01

    To design a new system for the in vivo phosphorylation of proteins in Escherichia coli using the co-expression of the α-subunit of casein kinase II (CKIIα) and a target protein, (Nanofitin) fused with a phosphorylatable tag. The level of the co-expressed CKIIα was controlled by the arabinose promoter and optimal phosphorylation was obtained with 2 % (w/v) arabinose as inductor. The effectiveness of the phosphorylation system was demonstrated by electrophoretic mobility shift assay (NUT-PAGE) and staining with a specific phosphoprotein-staining gel. The resulting phosphorylated tag was also used to purify the phosphoprotein by immobilized metal affinity chromatography, which relies on the specific interaction of phosphate moieties with Fe(III). The use of a single tag for both the purification and protein array anchoring provides a simple and straightforward system for protein analysis.

  13. Migration and spawning of radio-tagged zulega Prochilodus argenteus in a dammed Brazilian river

    Science.gov (United States)

    Godinho, Alexandre L.; Kynard, B.

    2006-01-01

    It is difficult for agencies to evaluate the impacts of the many planned dams on Sa??o Francisco River, Brazil, migratory fishes because fish migrations are poorly known. We conducted a study on zulega Prochilodus argenteus, an important commercial and recreational fish in the Sa??o Francisco River, to identify migrations and spawning areas and to determine linear home range. During two spawning seasons (2001-2003), we radio-tagged fish in three main-stem reaches downstream of Tre??s Marias Dam (TMD), located at river kilometer (rkm) 2,109. We tagged 10 fish at Tre??s Marias (TM), which is 5 km downstream of TMD; 12 fish at Pontal, which is 28 km downstream of TMD and which includes the mouth of the Abaete?? River, and 10 fish at Cilga, which is 45 km downstream of TMD. Late-stage (ripe) adults tagged in each area during the spawning season remained at or near the tagging site, except for four Cilga fish that went to Pontal and probably spawned. The Pontal area at the Abaete?? River mouth was the most important spawning site we found. Prespawning fish moved back and forth between main-stem staging areas upstream of the Abaete?? River mouth and Pontal for short visits. These multiple visits were probably needed as ripe fish waited for spawning cues from a flooding Abaete?? River. Some fish homed to prespaw ning staging areas, spawning areas, and nonspawning areas. The migratory style of zulega was dualistic, with resident and migratory fish. Total linear home range was also dualistic, with small (<26-km) and large (53-127-km) ranges. The locations of spawning areas and home ranges suggest that the Pontal group (which includes Cilga fish) is one population that occupies about 110 km. The Pontal population overlaps a short distance with a population located downstream of Cilga. Movements of late-stage TM adults suggest that the TM group is a separate population, possibly with connections to populations upstream of TMD. ?? Copyright by the American Fisheries Society

  14. N-terminal processing of affinity-tagged recombinant proteins purified by IMAC procedures.

    Science.gov (United States)

    Mooney, Jane T; Fredericks, Dale P; Christensen, Thorkild; Bruun Schiødt, Christine; Hearn, Milton T W

    2015-07-01

    The ability of a new class of metal binding tags to facilitate the purification of recombinant proteins, exemplified by the tagged glutathione S-transferase and human growth hormone, from Escherichia coli fermentation broths and lysates has been further investigated. These histidine-containing tags exhibit high affinity for borderline metal ions chelated to the immobilised ligand, 1,4,7-triazacyclononane (tacn). The use of this tag-tacn immobilised metal ion affinity chromatography (IMAC) system engenders high selectivity with regard to host cell protein removal and permits facile tag removal from the E. coli-expressed recombinant protein. In particular, these tags were specifically designed to enable their efficient removal by the dipeptidyl aminopeptidase 1 (DAP-1), thus capturing the advantages of high substrate specificity and rates of cleavage. MALDI-TOF MS analysis of the cleaved products from the DAP-1 digestion of the recombinant N-terminally tagged proteins confirmed the complete removal of the tag within 4-12 h under mild experimental conditions. Overall, this study demonstrates that the use of tags specifically designed to target tacn-based IMAC resins offers a comprehensive and flexible approach for the purification of E. coli-expressed recombinant proteins, where complete removal of the tag is an essential prerequisite for subsequent application of the purified native proteins in studies aimed at delineating the molecular and cellular basis of specific biological processes. Copyright © 2015 John Wiley & Sons, Ltd.

  15. Principle and design of hatch operation system for fish migrating upstream-downstream of the discharge sill

    Directory of Open Access Journals (Sweden)

    Răzvan G.Voicu

    2016-09-01

    Full Text Available To improve fish migration and restore local lotic ecosystems, it is necessary to consider the various current hydro-construction methods. There have been partial successes in systems that improve fish passage over various obstacles but new approaches that present more efficient solutions and international collaboration among specialists are required. This article deals with European themes of great interest; issues related to interruption of longitudinal connectivity in heavily modified water courses, and improving the ecological status of damaged lotic systems, with the goal of achieving the environmental objectives set forth by the Water Framework Directive 2000/60/EC (WFD and the national legislative framework regarding water policy (which reflects the European directives Water Law no. 107, with subsequent amendments, NT No. / 2006 OM 1163/2007, and discusses the obligation to ensure that construction works protect fish migration to maintain ecological balance in the reservoirs. It is possible to use this system in almost any fish-bearing stream, both upstream and downstream of maximum circulation, which makes the system an excellent solution for assisting and improving migration.

  16. Evaluation of partial water reuse systems used for Atlantic salmon smolt production at the White River National Fish Hatchery

    Science.gov (United States)

    Eight of the existing 9.1 m (30 ft) diameter circular culture tanks at the White River National Fish Hatchery in Bethel, Vermont, were retrofitted and plumbed into two 8,000 L/min partial water reuse systems to help meet the region's need for Atlantic salmon (Salmo salar) smolt production. The part...

  17. Manipulations of the reproductive system of fishes by means of exogenous chemicals

    Science.gov (United States)

    Patino, R.

    1997-01-01

    Environmental control of reproductive activity of captive fish is feasible (or potentially feasible) but, with few exceptions, is currently impractical for most species. Therefore, chemical methods of manipulating reproductive activity continue to be widely used in fish production operations worldwide. However, the control of fish reproduction in captivity cannot be exercised without regard to adequate environmental conditions, which can differ markedly for different species. This review provides a synopsis of relevant aspects of fish reproductive physiology and addresses current and promising future chemical methods of sex control, gonadal recrudescence, and spawning. Most research on the control of reproduction in fishes has focused on female physiology because ovarian development and maturation are easily disturbed by environmental stressors. Control of sex ratios by steroid treatment has become a well-established technique for several fish species, but the technique continues to be problematic in some cases. Final gonadal growth and spawning usually can be achieved by implant treatment with gonadotropin-releasing hormone analogs (GnRHa), which in some species have to be applied in combination with dopamine antagonists to enhance responsiveness to GnRHa. However, efforts to accelerate gonadal recrudescence and maturational competence by chemical means have yielded mixed results, reflecting a relative lack of understanding of the basic physiological and biochemic mechanisms controlling these processes. The potential benefits of using reproductive pheromone, to manipulate gonadal development and spawning has been demonstrated in a few species, but further research is needed to determine whether this technique is applicable to fish culture. Because a reliable supply of young fish is critical for the expansion and diversification of fish culture operations, the use of chemicals in combination with adequate environmental conditions to contain gametogenesis and

  18. Fish and aquatic habitat conservation in South America: a continental overview with emphasis on neotropical systems.

    Science.gov (United States)

    Barletta, M; Jaureguizar, A J; Baigun, C; Fontoura, N F; Agostinho, A A; Almeida-Val, V M F; Val, A L; Torres, R A; Jimenes-Segura, L F; Giarrizzo, T; Fabré, N N; Batista, V S; Lasso, C; Taphorn, D C; Costa, M F; Chaves, P T; Vieira, J P; Corrêa, M F M

    2010-06-01

    Fish conservation in South America is a pressing issue. The biodiversity of fishes, just as with all other groups of plants and animals, is far from fully known. Continuing habitat loss may result in biodiversity losses before full species diversity is known. In this review, the main river basins of South America (Magdalena, Orinoco, Amazon and Paraná-La Plata system), together with key aquatic habitats (mangrove-fringed estuaries of the tropical humid, tropical semi-arid and subtropical regions) are analysed in terms of their characteristics and main concerns. Habitat loss was the main concern identified for all South American ecosystems. It may be caused by damming of rivers, deforestation, water pollution, mining, poor agricultural practice or inadequate management practice. Habitat loss has a direct consequence, which is a decrease in the availability of living resources, a serious social and economic issue, especially for South American nations which are all developing countries. The introduction of exotic species and overfishing were also identified as widespread across the continent and its main freshwater, coastal and marine ecosystems. Finally, suggestions are made to find ways to overcome these problems. The main suggestion is a change of paradigm and a new design for conservation actions, starting with integrated research and aiming at the co-ordinated and harmonized management of the main transboundary waters of the continent. The actions would be focused on habitat conservation and social rescue of the less well-off populations of indigenous and non-indigenous peoples. Energy and freshwater demands will also have to be rescaled in order to control habitat loss.

  19. Comparative morphology of the mechanosensory lateral line system in a clade of New Zealand triplefin fishes.

    Science.gov (United States)

    Wellenreuther, Maren; Brock, Michelle; Montgomery, John; Clements, Kendall D

    2010-01-01

    The mechanoreceptive lateral line system in fishes detects hydrodynamic stimuli and plays a critical role in many fundamental behaviours, including orientation to water currents and the detection of stationary objects, prey and predators. Interspecific variation in lateral line structure may result from a process of functional adaptation, with the background level of hydrodynamic activity proposed as an important selective pressure. Here we use the eight species of the ecologically diverse New Zealand marine triplefin fish of the genus Forsterygion and one species from the sister genus Notoclinops to investigate interspecific differences in lateral line morphology and to assess the relationship between lateral line characteristics and exposure to wave energy (fetch/depth ratio). Overall, the results show that lateral line traits are divergent between species, and these differences could in part be related to the wave exposure of the habitats that the species occupy. Specifically, numbers of canal neuromasts differed significantly between species, and most canal groupings increased in neuromast number with fetch/depth ratio, while the number and area of some superficial neuromast groupings decreased significantly with exposure. Distribution of superficial neuromasts along the trunk in the semi-pelagic and paedomorphic species F. maryannae differed from the other, demersal species, which may be associated with the unique lifestyle of this species and/or developmental processes. Canal architecture also differed considerably between species, but displayed no relationship with fetch/depth ratio. The results from this study indicate that some interspecific differences in lateral line organs may be a by-product of selection for habitat divergence. Future work should explore additional causal factors that might have influenced the evolution of lateral morphology in these species, including phylogenetic and allometric effects. Copyright 2010 S. Karger AG, Basel.

  20. The accumulation and distribution of 60Co in carp (Cyprinus carpio) in water-fish compartment system

    International Nuclear Information System (INIS)

    Poppy Intan Tjahaja; Putu Sukmabuana; Eko Susanto

    2012-01-01

    In relation with nuclear safety assessment, the parameter of radionuclide transfer in the environment is significantly needed for internal doses estimation received by public trough environment - food product - human pathways. International Atomic Energy Agency (IAEA) has published the transfer parameter data for temperate zone in Technical Report Series 472. In order to complete the IAEA data, especially for tropical region, the accumulation and distribution of 60 Co in carp (Cyprinus carpio) was experimentally studied based on the water-fish compartment system. The carp were cultured in 500L water containing 60 Co of about 30 Bq.mL -1 in a water tank. The 60 Co concentration in fish and water were measured using gamma spectrometer. The 60 Co was accumulated and distributed in the fish tissues with the concentration ratio (CR) of 3.08 mL.g -1 1.55 mL.g -1 and 1.14 mL.g -1 for internal organs, bones, and muscle, respectively. The CR of 60 Co in the fish will be useful in internal radiation dose estimation to human trough water-fish-human pathway, and will also complete the IAEA transfer parameter data for tropical region. (author)

  1. ATLAS boosted object tagging 2

    CERN Document Server

    Caudron, Julien; The ATLAS collaboration

    2015-01-01

    A detailed study into the optimal techniques for identifying boosted hadronically decaying W or Z bosons is presented. Various algorithms for reconstructing, grooming and tagging bosonic jets are compared for W bosons with a wide range of transverse momenta using 8 TeV data and 8 TeV and 13 TeV MC simulations. In addition, given that a hadronic jet has been identified as resulting from the hadronic decay of a W or Z, a technique is developed to discriminate between W and Z bosons. The modeling of the tagging variables used in this technique is studied using 8 TeV pp collision data and systematic uncertainties for the tagger efficiency and fake rates are evaluated.

  2. Predicting floods with Flickr tags.

    Science.gov (United States)

    Tkachenko, Nataliya; Jarvis, Stephen; Procter, Rob

    2017-01-01

    Increasingly, user generated content (UGC) in social media postings and their associated metadata such as time and location stamps are being used to provide useful operational information during natural hazard events such as hurricanes, storms and floods. The main advantage of these new sources of data are twofold. First, in a purely additive sense, they can provide much denser geographical coverage of the hazard as compared to traditional sensor networks. Second, they provide what physical sensors are not able to do: By documenting personal observations and experiences, they directly record the impact of a hazard on the human environment. For this reason interpretation of the content (e.g., hashtags, images, text, emojis, etc) and metadata (e.g., keywords, tags, geolocation) have been a focus of much research into social media analytics. However, as choices of semantic tags in the current methods are usually reduced to the exact name or type of the event (e.g., hashtags '#Sandy' or '#flooding'), the main limitation of such approaches remains their mere nowcasting capacity. In this study we make use of polysemous tags of images posted during several recent flood events and demonstrate how such volunteered geographic data can be used to provide early warning of an event before its outbreak.

  3. Approximation properties of haplotype tagging

    Directory of Open Access Journals (Sweden)

    Dreiseitl Stephan

    2006-01-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs are locations at which the genomic sequences of population members differ. Since these differences are known to follow patterns, disease association studies are facilitated by identifying SNPs that allow the unique identification of such patterns. This process, known as haplotype tagging, is formulated as a combinatorial optimization problem and analyzed in terms of complexity and approximation properties. Results It is shown that the tagging problem is NP-hard but approximable within 1 + ln((n2 - n/2 for n haplotypes but not approximable within (1 - ε ln(n/2 for any ε > 0 unless NP ⊂ DTIME(nlog log n. A simple, very easily implementable algorithm that exhibits the above upper bound on solution quality is presented. This algorithm has running time O((2m - p + 1 ≤ O(m(n2 - n/2 where p ≤ min(n, m for n haplotypes of size m. As we show that the approximation bound is asymptotically tight, the algorithm presented is optimal with respect to this asymptotic bound. Conclusion The haplotype tagging problem is hard, but approachable with a fast, practical, and surprisingly simple algorithm that cannot be significantly improved upon on a single processor machine. Hence, significant improvement in computatational efforts expended can only be expected if the computational effort is distributed and done in parallel.

  4. b-tagging in DELPHI at LEP

    CERN Document Server

    Abdallah, J; Adam, W; Adye, T; Adzic, P; Albrecht, T; Alderweireld, T; Alemany-Fernandez, R; Allmendinger, T; Allport, P P; Almehed, S; Amaldi, Ugo; Amapane, N; Amato, S; Anashkin, E; Andreazza, A; Andringa, S; Anjos, N; Antilogus, P; Apel, W D; Arnoud, Y; Ask, S; Åsman, B; Augustin, J E; Augustinus, A; Baillon, Paul; Ballestrero, A; Bambade, P; Barbier, R; Bardin, Dimitri Yuri; Barker, G; Baroncelli, A; Bates, M; Battaglia, Marco; Baubillier, M; Becks, K H; Begalli, M; Behrmann, A; Benekos, N C; Benvenuti, Alberto C; Bérat, C; Berggren, M; Berntzon, L; Bertrand, D; Besançon, M; Besson, N; Bibby, J; Biffi, P; Bloch, D; Blom, M; Bonesini, M; Boonekamp, M; Booth, P S L; Borisov, G; Botner, O; Bouquet, B; Bowcock, T J V; Boyko, I; Bracko, M; Branchini, P; Brenner, R; Brodet, E; Brückman, P; Brunet, J M; Bugge, L; Buschmann, P; Caccia, M; Calvi, M; Camporesi, T; Canale, V; Carena, F; Castro, N; Cavallo, F R; Chabaud, V; Chapkin, M M; Charpentier, P; Checchia, P; Chierici, R; Shlyapnikov, P; Chudoba, J; Chung, S U; Cieslik, K; Collins, P; Contri, R; Cosme, G; Cossutti, F; Costa, M J; Couchot, F; Crawley, B; Crennell, D J; Cuevas-Maestro, J; D'Almagne, B; D'Hondt, J; Dalmau, J; Da Silva, T; Da Silva, W; Della Ricca, G; De Angelis, A; de Boer, Wim; De Clercq, C; De Lotto, B; De Maria, N; De Min, A; De Paula, L S; Di Ciaccio, Lucia; Dijkstra, H; Di Simone, A; Doroba, K; Drees, J; Dris, M; Eigen, G; Ekelöf, T J C; Ellert, M; Elsing, M; Espirito-Santo, M C; Fanourakis, G K; Fassouliotis, D; Feindt, M; Fernández, J; Ferrer, A; Ferro, F; Flagmeyer, U; Föth, H; Fokitis, E; Fulda-Quenzer, F; Fuster, J A; Gandelman, M; García, C; Gavillet, P; Gazis, E N; Geralis, T; Gokieli, R; Golob, B; Gómez-Cadenas, J J; Gómez-Ceballos, G; Gonçalves, P; Graziani, E; Grosdidier, G; Grzelak, K; Guy, J; Haag, C; Hallgren, A; Hamacher, K; Hamilton, K; Hansen, J; Haug, S; Hauler, F; Hedberg, V; Hennecke, M; Hernando, J A; Herr, H; Heuser, J M; Holmgren, S O; Holt, P J; Houlden, M A; Hultqvist, K; Jackson, J N; Jalocha, P; Jarlskog, C; Jarlskog, G; Jarry, P; Jeans, D; Johansson, E K; Johansson, P D; Jonsson, P; Joram, C; Jungermann, L; Kapusta, F; Karlsson, M; Katsanevas, S; Katsoufis, E C; Keränen, R; Kernel, G; Kersevan, Borut P; Kiiskinen, A P; King, B T; Kjaer, N J; Kluit, P; Kokkinias, P; Kourkoumelis, C; Kuznetsov, O; Krumshtein, Z; Kucharczyk, M; Kucewicz, W; Kurowska, J; Lamsa, J; Leder, G; Ledroit, F; Leinonen, L; Leitner, R; Lemonne, J; Lepeltier, V; Lesiak, T; Liebig, W; Liko, D; Lipniacka, A; Lopes, J H; López, J M; Loukas, D; Lutz, P; Lyons, L; MacNaughton, J; Malek, A; Maltezos, S; Mandl, F; Marco, J; Marco, R; Maréchal, B; Margoni, M; Marin, J C; Mariotti, C; Markou, A; Martínez-Rivero, C; Martínez-Vidal, F; Masik, J; Mastroyiannopoulos, N; Matorras, F; Matteuzzi, C; Mazzucato, F; Mazzucato, M; McNulty, R; Meroni, C; Meyer, W T; Migliore, E; Mitaroff, W A; Mjörnmark, U; Moa, T; Moch, M; Mönig, K; Monge, R; Montenegro, J; Moraes, D; Moreno, S; Morettini, P; Müller, U; Münich, K; Mulders, M; Mundim, L; Murray, W; Muryn, B; Myatt, Gerald; Myklebust, T; Nassiakou, M; Navarria, Francesco Luigi; Nawrocki, K; Nicolaidou, R; Niezurawski, P; Nikolenko, M; Nomerotski, A; Norman, A; Nygren, A; Oblakowska-Mucha, A; Obraztsov, V F; Olshevskii, A G; Onofre, A; Orava, Risto; Österberg, K; Ouraou, A; Oyanguren, A; Paganoni, M; Paiano, S; Palacios, J P; Palka, H; Papadopoulou, T D; Pape, L; Parkes, C; Parodi, F; Parzefall, U; Passeri, A; Passon, O; Peralta, L; Perepelitsa, V F; Perrotta, A; Petrolini, A; Piedra, J; Pieri, L; Pierre, F; Pimenta, M; Piotto, E; Podobnik, T; Poireau, V; Pol, M E; Polok, G; Poropat, P; Pozdnyakov, V; Pukhaeva, N; Pullia, Antonio; Rames, J; Ramler, L; Read, A; Rebecchi, P; Rehn, J; Reid, D; Reinhardt, R; Renton, P B; Richard, F; Rídky, J; Rivero, M; Rodríguez, D; Romero, A; Ronchese, P; Rosenberg, E I; Roudeau, Patrick; Rovelli, T; Ruhlmann-Kleider, V; Ryabtchikov, D; Sadovskii, A; Salmi, L; Salt, J; Savoy-Navarro, A; Schwickerath, U; Segar, A; Sekulin, R L; Siebel, M; Sissakian, A N; Smadja, G; Smirnova, O G; Sokolov, A; Sopczak, A; Sosnowski, R; Spassoff, Tz; Stanitzki, M; Stavitski, I; Stocchi, A; Strauss, J; Stugu, B; Szczekowski, M; Szeptycka, M; Szumlak, T; Tabarelli de Fatis, T; Taffard, A C; Tegenfeldt, F; Timmermans, J; Tinti, N; Tkatchev, L G; Tobin, M; Todorovova, S; Tomaradze, A G; Tomé, B; Tonazzo, A; Tortosa, P; Travnicek, P; Treille, D; Trischuk, W; Tristram, G; Trochimczuk, M; Troncon, C; Turluer, M L; Tyapkin, I A; Tyapkin, P; Tyndel, M; Tzamarias, S; Uvarov, V; Valenti, G; van Dam, P; Van Eldik, J; Van Lysebetten, A; Van Remortel, N; Van Vulpen, I B; Vegni, G; Veloso, F; Venus, W A; Verbeure, F; Verdier, P; Verzi, V; Vilanova, D; Vitale, L; Vrba, V; Wahlen, H; Washbrook, A J; Weilhammer, Peter; Weiser, C; Wicke, D; Wickens, J H; Wilkinson, G; Winter, M; Witek, M; Yushchenko, O P; Zalewska-Bak, A; Zalewski, Piotr; Zavrtanik, D; Zimin, N I; Zinchenko, A I; Zupan, M

    2004-01-01

    The standard method used for tagging b-hadrons in the DELPHI experiment at the CERN LEP Collider is discussed in detail. The main ingredient of b-tagging is the impact parameters of tracks, which relies mostly on the vertex detector. Additional information, such as the mass of particles associated to a secondary vertex, significantly improves the selection efficiency and the background suppression. The paper describes various discriminating variables used for the tagging and the procedure of their combination. In addition, applications of b-tagging to some physics analyses, which depend crucially on the performance and reliability of b-tagging, are described briefly.

  5. Socio-ecological analysis of the artisanal fishing system on Easter Island

    Directory of Open Access Journals (Sweden)

    Eleuterio Yáñez

    2014-10-01

    Full Text Available This paper introduces a socio-ecological analysis of the artisanal fisheries system on Easter Island (27°07'S, 109°22'W through the identification and interaction of stakeholders. It also comprises a structural analysis of the system aiming to identify any key issues and then propose research and development programs for multiple fisheries that will contribute to their sustainable development. The methodology is divided into four stages: i identification of issues with stakeholder (fishers, government workers and expert scientists participation, ii analysis of a structural matrix consisting of a direct causality study of the symmetric, structural and binary matrix based on socio-ecological issues allowing the calculation of the level of influence and dependence of each issue, iii identification of key issues with an influence/dependency diagram, and iv proposal of research and development programs according to the needs and opportunities identified in the previous stages. The Easter Island artisanal fishing system is used as a case study for this methodological approach. Thus, the fishers identified 108 issues, which were then grouped by similarity, reducing the number to 27 global issues, of which seven were identified as key. Surveyed local and central government workers and expert scientists identified 7, 2 and 5 issues, respectively. Finally, research and development programs are proposed that will encourage a series of changes to the fisheries situation on the island, in order to resolve issues and promote their sustainable development.

  6. A Framework Based on a Systems Approach to Developing Safety Indicators in Fish Farming

    Directory of Open Access Journals (Sweden)

    Siri Mariane Holen

    2018-05-01

    Full Text Available The fish farming industry is one of the industries in Norway with the highest occupational fatality and injury rate. Despite the serious health, safety, and environmental issues in the industry, little is done to measure changes in safety over time beyond the traditional Lost Time Injury (LTI registrations. In this article the objective is twofold; (i to propose a framework for developing safety indicators based on Systems-Theoretic Process Analysis (STPA, and (ii to apply the framework to find indicators relevant for hazards in operations where subcontractors participate. STPA uses a hierarchical portrayal of the system in focus, in contrast to sequential models, and views safety as a control problem. It is believed that a systemic approach to indicator development better captures the complex safety challenges in aquaculture. Thirteen indicators are identified within areas such as maintenance, training, and planning. The indicators identified may function as a basis for decisions and actions that must be undertaken to ensure safe operations.

  7. The Collective Knowledge of Social Tags: Direct and Indirect Influences on Navigation, Learning, and Information Processing

    Science.gov (United States)

    Cress, Ulrike; Held, Christoph; Kimmerle, Joachim

    2013-01-01

    Tag clouds generated in social tagging systems can capture the collective knowledge of communities. Using as a basis spreading activation theories, information foraging theory, and the co-evolution model of cognitive and social systems, we present here a model for an "extended information scent," which proposes that both collective and individual…

  8. Interference Helps to Equalize the Read Range and Reduce False Positives of Passive RFID Tags

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund

    2012-01-01

    In various applications of RFID systems a reader should reliably get the ID of the tags that are within a bounded proximity region, termed the interrogation zone. This gives rise to two types of errors 1) False Negative Detections (FNDs), when tags within the intended interrogation zone cannot...... interference enables design of well-defined interrogation zones for passive RFID systems....

  9. Matching breeding goals with farming systems to enhance the sustainability of fish farming

    NARCIS (Netherlands)

    Besson, Mathieu

    2017-01-01

    Fish farming is growing but is also facing challenges regarding economic viability and environmental sustainability. Selective breeding could enhance the sustainability of fish farming by changing animal performances. Thus, our aim was to develop sustainable breeding goals by using economic (EV)

  10. Reliable and inexpensive expression of large, tagged, exogenous proteins in murine bone marrow-derived macrophages using a second generation lentiviral system

    Directory of Open Access Journals (Sweden)

    Matthew R. Miller

    2015-08-01

    Full Text Available Over the past two decades, researchers have struggled to efficiently express foreign DNA in primary macrophages, impeding research progress. The applications of lipofection, electroporation, microinjection, and viral-mediated transfer typically result in disruptions in macrophage differentiation and function, low expression levels of exogenous proteins, limited efficiency and high cell mortality. In this report, after extensive optimization, we present a method of expressing large tagged proteins at high efficiency, consistency, and low cost using lentiviral infection. This method utilizes laboratory-propagated second generation plasmids to produce efficient virus that can be stored for later use. The expression of proteins up to 150 kDa in size is achieved in 30–70% of cells while maintaining normal macrophage differentiation and morphology as determined by fluorescence microscopy and Western blot analysis. This manuscript delineates the reagents and methods used to produce lentivirus to express exogenous DNA in murine bone marrow-derived macrophages sufficient for single cell microscopy as well as functional assays requiring large numbers of murine bone marrow-derived macrophages.

  11. Habitat use and movement patterns of bull sharks Carcharhinus leucas determined using pop-up satellite archival tags.

    Science.gov (United States)

    Carlson, J K; Ribera, M M; Conrath, C L; Heupel, M R; Burgess, G H

    2010-08-01

    Habitat use, movement and residency of bull sharks Carcharhinus leucas were determined using satellite pop-up archival transmitting (PAT) tags throughout coastal areas in the U.S., Gulf of Mexico and waters off the south-east U.S. From 2005 to 2007, 18 fish (mean size = 164 cm fork length, L(F)) were tagged over all seasons. Fish retained tags for up to 85 days (median = 30 days). Based on geolocation data from initial tagging location to pop-off location, C. leucas generally travelled c. 5-6 km day(-1) and travelled an average of 143.6 km. Overall, mean proportions of time at depth revealed C. leucas spent the majority of their time in waters freshwater inflow.

  12. Characterization of microbial associations with methanotrophic archaea and sulfate-reducing bacteria through statistical comparison of nested Magneto-FISH enrichments

    Directory of Open Access Journals (Sweden)

    Elizabeth Trembath-Reichert

    2016-04-01

    Full Text Available Methane seep systems along continental margins host diverse and dynamic microbial assemblages, sustained in large part through the microbially mediated process of sulfate-coupled Anaerobic Oxidation of Methane (AOM. This methanotrophic metabolism has been linked to consortia of anaerobic methane-oxidizing archaea (ANME and sulfate-reducing bacteria (SRB. These two groups are the focus of numerous studies; however, less is known about the wide diversity of other seep associated microorganisms. We selected a hierarchical set of FISH probes targeting a range of Deltaproteobacteria diversity. Using the Magneto-FISH enrichment technique, we then magnetically captured CARD-FISH hybridized cells and their physically associated microorganisms from a methane seep sediment incubation. DNA from nested Magneto-FISH experiments was analyzed using Illumina tag 16S rRNA gene sequencing (iTag. Enrichment success and potential bias with iTag was evaluated in the context of full-length 16S rRNA gene clone libraries, CARD-FISH, functional gene clone libraries, and iTag mock communities. We determined commonly used Earth Microbiome Project (EMP iTAG primers introduced bias in some common methane seep microbial taxa that reduced the ability to directly compare OTU relative abundances within a sample, but comparison of relative abundances between samples (in nearly all cases and whole community-based analyses were robust. The iTag dataset was subjected to statistical co-occurrence measures of the most abundant OTUs to determine which taxa in this dataset were most correlated across all samples. Many non-canonical microbial partnerships were statistically significant in our co-occurrence network analysis, most of which were not recovered with conventional clone library sequencing, demonstrating the utility of combining Magneto-FISH and iTag sequencing methods for hypothesis generation of associations within complex microbial communities. Network analysis pointed to

  13. Characterization of microbial associations with methanotrophic archaea and sulfate-reducing bacteria through statistical comparison of nested Magneto-FISH enrichments.

    Science.gov (United States)

    Trembath-Reichert, Elizabeth; Case, David H; Orphan, Victoria J

    2016-01-01

    Methane seep systems along continental margins host diverse and dynamic microbial assemblages, sustained in large part through the microbially mediated process of sulfate-coupled Anaerobic Oxidation of Methane (AOM). This methanotrophic metabolism has been linked to consortia of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB). These two groups are the focus of numerous studies; however, less is known about the wide diversity of other seep associated microorganisms. We selected a hierarchical set of FISH probes targeting a range of Deltaproteobacteria diversity. Using the Magneto-FISH enrichment technique, we then magnetically captured CARD-FISH hybridized cells and their physically associated microorganisms from a methane seep sediment incubation. DNA from nested Magneto-FISH experiments was analyzed using Illumina tag 16S rRNA gene sequencing (iTag). Enrichment success and potential bias with iTag was evaluated in the context of full-length 16S rRNA gene clone libraries, CARD-FISH, functional gene clone libraries, and iTag mock communities. We determined commonly used Earth Microbiome Project (EMP) iTAG primers introduced bias in some common methane seep microbial taxa that reduced the ability to directly compare OTU relative abundances within a sample, but comparison of relative abundances between samples (in nearly all cases) and whole community-based analyses were robust. The iTag dataset was subjected to statistical co-occurrence measures of the most abundant OTUs to determine which taxa in this dataset were most correlated across all samples. Many non-canonical microbial partnerships were statistically significant in our co-occurrence network analysis, most of which were not recovered with conventional clone library sequencing, demonstrating the utility of combining Magneto-FISH and iTag sequencing methods for hypothesis generation of associations within complex microbial communities. Network analysis pointed to many co

  14. Effect of food microstructure on growth dynamics of Listeria monocytogenes in fish-based model systems.

    Science.gov (United States)

    Verheyen, Davy; Bolívar, Araceli; Pérez-Rodríguez, Fernando; Baka, Maria; Skåra, Torstein; Van Impe, Jan F

    2018-06-01

    Traditionally, predictive growth models for food pathogens are developed based on experiments in broth media, resulting in models which do not incorporate the influence of food microstructure. The use of model systems with various microstructures is a promising concept to get more insight into the influence of food microstructure on microbial dynamics. By means of minimal variation of compositional and physicochemical factors, these model systems can be used to study the isolated effect of certain microstructural aspects on microbial growth, survival and inactivation. In this study, the isolated effect on microbial growth dynamics of Listeria monocytogenes of two food microstructural aspects and one aspect influenced by food microstructure were investigated, i.e., the nature of the food matrix, the presence of fat droplets, and microorganism growth morphology, respectively. To this extent, fish-based model systems with various microstructures were used, i.e., a liquid, a second more viscous liquid system containing xanthan gum, an emulsion, an aqueous gel, and a gelled emulsion. Growth experiments were conducted at 4 and 10 °C, both using homogeneous and surface inoculation (only for the gelled systems). Results regarding the influence of the growth morphology indicated that the lag phase of planktonic cells in the liquid system was similar to the lag phase of submerged colonies in the xanthan system. The lag phase of submerged colonies in each gelled system was considerably longer than the lag phase of surface colonies on these respective systems. The maximum specific growth rate of planktonic cells in the liquid system was significantly lower than for submerged colonies in the xanthan system at 10 °C, while no significant differences were observed at 4 °C. The maximum cell density was higher for submerged colonies than for surface colonies. The nature of the food matrix only exerted an influence on the maximum specific growth rate, which was

  15. Effect of anaesthesia with clove oil in fish (review).

    Science.gov (United States)

    Javahery, Susan; Nekoubin, Hamed; Moradlu, Abdolmajid Haji

    2012-12-01

    Clove oil is an effective, local and natural anaesthetic. Many hatcheries and research studies use clove oil to immobilize fish for handling, sorting, tagging, artificial reproduction procedures and surgery and to suppress sensory systems during invasive procedures. Clove oil may be more appropriate for use in commercial aquaculture situations. Improper clove oil use can decrease fish viability, distort physiological data or result in mortalities. Because animals may be anaesthetized by unskilled labourers and released in natural water bodies, training in the proper use of clove oil may decrease variability in recovery and experimental results and increase fish survival. Here, we briefly describe many aspects of clove oil, including the legal uses of it, anaesthesia mechanism and what is currently known about the preparation and behavioural and pathologic effects of the anaesthetic. We outline methods and precautions for administration and changes in fish behaviour during progressively deeper anaesthesia and discuss the physiological effects of clove oil, its potential for compromising fish health and effectiveness of water quality parameters.

  16. The origin and evolution of the surfactant system in fish: insights into the evolution of lungs and swim bladders.

    Science.gov (United States)

    Daniels, Christopher B; Orgeig, Sandra; Sullivan, Lucy C; Ling, Nicholas; Bennett, Michael B; Schürch, Samuel; Val, Adalberto Luis; Brauner, Colin J

    2004-01-01

    Several times throughout their radiation fish have evolved either lungs or swim bladders as gas-holding structures. Lungs and swim bladders have different ontogenetic origins and can be used either for buoyancy or as an accessory respiratory organ. Therefore, the presence of air-filled bladders or lungs in different groups of fishes is an example of convergent evolution. We propose that air breathing could not occur without the presence of a surfactant system and suggest that this system may have originated in epithelial cells lining the pharynx. Here we present new data on the surfactant system in swim bladders of three teleost fish (the air-breathing pirarucu Arapaima gigas and tarpon Megalops cyprinoides and the non-air-breathing New Zealand snapper Pagrus auratus). We determined the presence of surfactant using biochemical, biophysical, and morphological analyses and determined homology using immunohistochemical analysis of the surfactant proteins (SPs). We relate the presence and structure of the surfactant system to those previously described in the swim bladders of another teleost, the goldfish, and those of the air-breathing organs of the other members of the Osteichthyes, the more primitive air-breathing Actinopterygii and the Sarcopterygii. Snapper and tarpon swim bladders are lined with squamous and cuboidal epithelial cells, respectively, containing membrane-bound lamellar bodies. Phosphatidylcholine dominates the phospholipid (PL) profile of lavage material from all fish analyzed to date. The presence of the characteristic surfactant lipids in pirarucu and tarpon, lamellar bodies in tarpon and snapper, SP-B in tarpon and pirarucu lavage, and SPs (A, B, and D) in swim bladder tissue of the tarpon provide strong evidence that the surfactant system of teleosts is homologous with that of other fish and of tetrapods. This study is the first demonstration of the presence of SP-D in the air-breathing organs of nonmammalian species and SP-B in actinopterygian

  17. Atlantic menhaden adult tagging study

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Atlantic menhaden are a schooling forage fish species, which are subject to a large commercial purse seine fishery. Atlantic menhaden are harvested for reduction...

  18. Precision Electrophile Tagging in Caenorhabditis elegans.

    Science.gov (United States)

    Long, Marcus J C; Urul, Daniel A; Chawla, Shivansh; Lin, Hong-Yu; Zhao, Yi; Haegele, Joseph A; Wang, Yiran; Aye, Yimon

    2018-01-16

    Adduction of an electrophile to privileged sensor proteins and the resulting phenotypically dominant responses are increasingly appreciated as being essential for metazoan health. Functional similarities between the biological electrophiles and electrophilic pharmacophores commonly found in covalent drugs further fortify the translational relevance of these small-molecule signals. Genetically encodable or small-molecule-based fluorescent reporters and redox proteomics have revolutionized the observation and profiling of cellular redox states and electrophile-sensor proteins, respectively. However, precision mapping between specific redox-modified targets and specific responses has only recently begun to be addressed, and systems tractable to both genetic manipulation and on-target redox signaling in vivo remain largely limited. Here we engineer transgenic Caenorhabditis elegans expressing functional HaloTagged fusion proteins and use this system to develop a generalizable light-controlled approach to tagging a prototypical electrophile-sensor protein with native electrophiles in vivo. The method circumvents issues associated with low uptake/distribution and toxicity/promiscuity. Given the validated success of C. elegans in aging studies, this optimized platform offers a new lens with which to scrutinize how on-target electrophile signaling influences redox-dependent life span regulation.

  19. UTag: Long-range Ultra-wideband Passive Radio Frequency Tags

    Energy Technology Data Exchange (ETDEWEB)

    Dowla, F

    2007-03-14

    Long-range, ultra-wideband (UWB), passive radio frequency (RF) tags are key components in Radio Frequency IDentification (RFID) system that will revolutionize inventory control and tracking applications. Unlike conventional, battery-operated (active) RFID tags, LLNL's small UWB tags, called 'UTag', operate at long range (up to 20 meters) in harsh, cluttered environments. Because they are battery-less (that is, passive), they have practically infinite lifetimes without human intervention, and they are lower in cost to manufacture and maintain than active RFID tags. These robust, energy-efficient passive tags are remotely powered by UWB radio signals, which are much more difficult to detect, intercept, and jam than conventional narrowband frequencies. The features of long range, battery-less, and low cost give UTag significant advantage over other existing RFID tags.

  20. Rapid and Parallel Adaptive Evolution of the Visual System of Neotropical Midas Cichlid Fishes.

    Science.gov (United States)

    Torres-Dowdall, Julián; Pierotti, Michele E R; Härer, Andreas; Karagic, Nidal; Woltering, Joost M; Henning, Frederico; Elmer, Kathryn R; Meyer, Axel

    2017-10-01

    Midas cichlid fish are a Central American species flock containing 13 described species that has been dated to only a few thousand years old, a historical timescale infrequently associated with speciation. Their radiation involved the colonization of several clear water crater lakes from two turbid great lakes. Therefore, Midas cichlids have been subjected to widely varying photic conditions during their radiation. Being a primary signal relay for information from the environment to the organism, the visual system is under continuing selective pressure and a prime organ system for accumulating adaptive changes during speciation, particularly in the case of dramatic shifts in photic conditions. Here, we characterize the full visual system of Midas cichlids at organismal and genetic levels, to determine what types of adaptive changes evolved within the short time span of their radiation. We show that Midas cichlids have a diverse visual system with unexpectedly high intra- and interspecific variation in color vision sensitivity and lens transmittance. Midas cichlid populations in the clear crater lakes have convergently evolved visual sensitivities shifted toward shorter wavelengths compared with the ancestral populations from the turbid great lakes. This divergence in sensitivity is driven by changes in chromophore usage, differential opsin expression, opsin coexpression, and to a lesser degree by opsin coding sequence variation. The visual system of Midas cichlids has the evolutionary capacity to rapidly integrate multiple adaptations to changing light environments. Our data may indicate that, in early stages of divergence, changes in opsin regulation could precede changes in opsin coding sequence evolution. © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  1. Disparity between state fish consumption advisory systems for Methylmercury and US Environmental Protection Agency recommendations: a case study of the South Central United States

    Science.gov (United States)

    Adams, Kimberly; Drenner, Ray W.; Chumchal, Matthew M.; Donato, David I.

    2015-01-01

    Fish consumption advisories are used to inform citizens in the United States about noncommercial game fish with hazardous levels of methylmercury (MeHg). The US Environmental Protection Agency (USEPA) suggests issuing a fish consumption advisory when concentrations of MeHg in fish exceed a human health screening value of 300 ng/g. However, states have authority to develop their own systems for issuing fish consumption advisories for MeHg. Five states in the south central United States (Arkansas, Louisiana, Mississippi, Oklahoma, and Texas) issue advisories for the general human population when concentrations of MeHg exceed 700 ng/g to 1000 ng/g. The objective of the present study was to estimate the increase in fish consumption advisories that would occur if these states followed USEPA recommendations. The authors used the National Descriptive Model of Mercury in Fish to estimate the mercury concentrations in 5 size categories of largemouth bass–equivalent fish at 766 lentic and lotic sites within the 5 states. The authors found that states in this region have not issued site-specific fish consumption advisories for most of the water bodies that would have such advisories if USEPA recommendations were followed. One outcome of the present study may be to stimulate discussion between scientists and policy makers at the federal and state levels about appropriate screening values to protect the public from the health hazards of consuming MeHg-contaminated game fish.

  2. Secure optionally passive RFID tag or sensor with external power source and data logging

    Science.gov (United States)

    Nekoogar, Faranak; Reynolds, Matthew; Lefton, Scott; Dowla, Farid; Twogood, Richard

    2016-05-31

    A secure optionally passive RFID tag or sensor system comprises a passive RFID tag having means for receiving radio signals from at least one base station and for transmitting radio signals to at least one base station, where the tag is capable of being powered exclusively by received radio energy, and an external power and data logging device having at least one battery and electronic circuitry including a digital memory configured for storing and recalling data. The external power and data logging device has a means for powering the tag, and also has a means.

  3. Development of an automated processing system for potential fishing zone forecast

    Science.gov (United States)

    Ardianto, R.; Setiawan, A.; Hidayat, J. J.; Zaky, A. R.

    2017-01-01

    The Institute for Marine Research and Observation (IMRO) - Ministry of Marine Affairs and Fisheries Republic of Indonesia (MMAF) has developed a potential fishing zone (PFZ) forecast using satellite data, called Peta Prakiraan Daerah Penangkapan Ikan (PPDPI). Since 2005, IMRO disseminates everyday PPDPI maps for fisheries marine ports and 3 days average for national areas. The accuracy in determining the PFZ and processing time of maps depend much on the experience of the operators creating them. This paper presents our research in developing an automated processing system for PPDPI in order to increase the accuracy and shorten processing time. PFZ are identified by combining MODIS sea surface temperature (SST) and chlorophyll-a (CHL) data in order to detect the presence of upwelling, thermal fronts and biological productivity enhancement, where the integration of these phenomena generally representing the PFZ. The whole process involves data download, map geo-process as well as layout that are carried out automatically by Python and ArcPy. The results showed that the automated processing system could be used to reduce the operator’s dependence on determining PFZ and speed up processing time.

  4. Development of the digestive system in larvae of the Neotropical fish Prochilodus argenteus (Characiformes, Prochilodontidae

    Directory of Open Access Journals (Sweden)

    Alcione Eneida Santos

    2016-01-01

    Full Text Available The development of the digestive system in larvae of the Neotropical fish P. argenteus was analyzed histologically. On the 3rd day after hatching, the digestive system comprised oropharyngeal cavity, esophagus and simple undifferentiated tube. Since secreting cells, positive to Alcian Blue (AB, were found in the esophagus, digestive activity in the initial phase had occurred. On the 18 and 28th days after hatching, the esophagus was positive for AB and Periodic Acid Schiff (PAS stain. The stomach was fully differentiated, with the cardiac, fundic and pyloric regions. Different regions of the epithelium were characterized by basic and acidic secreting cells (AB and PAS positive. On the 18 and 28th days after hatching, the intestine was long, coiled and divided into proximal, middle and distal segments with pyloric ceca. Secreting cells in different regions of the gut were either positive or negative for AB and PAS. Results showed that larvae of P. argenteus exhibited digestive activity on the third day after hatching, with fully differentiated stomach and intestines on the 18 and 28th days and their different regions featuring secreting cells.

  5. Predicting interactions among fishing, ocean warming, and ocean acidification in a marine system with whole-ecosystem models.

    Science.gov (United States)

    Griffith, Gary P; Fulton, Elizabeth A; Gorton, Rebecca; Richardson, Anthony J

    2012-12-01

    An important challenge for conservation is a quantitative understanding of how multiple human stressors will interact to mitigate or exacerbate global environmental change at a community or ecosystem level. We explored the interaction effects of fishing, ocean warming, and ocean acidification over time on 60 functional groups of species in the southeastern Australian marine ecosystem. We tracked changes in relative biomass within a coupled dynamic whole-ecosystem modeling framework that included the biophysical system, human effects, socioeconomics, and management evaluation. We estimated the individual, additive, and interactive effects on the ecosystem and for five community groups (top predators, fishes, benthic invertebrates, plankton, and primary producers). We calculated the size and direction of interaction effects with an additive null model and interpreted results as synergistic (amplified stress), additive (no additional stress), or antagonistic (reduced stress). Individually, only ocean acidification had a negative effect on total biomass. Fishing and ocean warming and ocean warming with ocean acidification had an additive effect on biomass. Adding fishing to ocean warming and ocean acidification significantly changed the direction and magnitude of the interaction effect to a synergistic response on biomass. The interaction effect depended on the response level examined (ecosystem vs. community). For communities, the size, direction, and type of interaction effect varied depending on the combination of stressors. Top predator and fish biomass had a synergistic response to the interaction of all three stressors, whereas biomass of benthic invertebrates responded antagonistically. With our approach, we were able to identify the regional effects of fishing on the size and direction of the interacting effects of ocean warming and ocean acidification. ©2012 Society for Conservation Biology.

  6. Using data storage tags to link otolith macrostructure in Baltic cod Gadus morhua with environmental conditions

    DEFF Research Database (Denmark)

    Hüssy, Karin; Nielsen, Birgitte; Mosegaard, Henrik

    2009-01-01

    of a strontium chloride solution. Based on environmental conditions experienced, fish were classified into different behavioural types: non-reproducing 'non-spawner', and 'spawner' undertaking spawning migrations. Otolith opacity, an indicator of otolith and fish somatic growth and condition, was examined...... in relation to these environmental drivers. Temperature was the only environmental variable with a significant effect, overlaying a strong size-related effect. The temperature effect was not uniform across behavioural types and spawning periods. Opacity showed a negative correlation with temperature......We examined otolith opacity of Baltic cod in relation to environmental conditions in order to evaluate the formation mechanisms of seasonal patterns used in age determination. Adult fish were tagged with data storage tags (DSTs) and a permanent mark was induced in the otoliths by injection...

  7. DNA barcoding discriminates freshwater fishes from southeastern Nigeria and provides river system-level phylogeographic resolution within some species.

    Science.gov (United States)

    Nwani, Christopher D; Becker, Sven; Braid, Heather E; Ude, Emmanuel F; Okogwu, Okechukwu I; Hanner, Robert

    2011-10-01

    Fishes are the main animal protein source for human beings and play a vital role in aquatic ecosystems and food webs. Fish identification can be challenging, especially in the tropics (due to high diversity), and this is particularly true for larval forms or fragmentary remains. DNA barcoding, which uses the 5' region of the mitochondrial cytochrome c oxidase subunit I (COI) as a target gene, is an efficient method for standardized species-level identification for biodiversity assessment and conservation, pending the establishment of reference sequence libraries. In this study, fishes were collected from three rivers in southeastern Nigeria, identified morphologically, and imaged digitally. DNA was extracted, PCR-amplified, and the standard barcode region was bidirectionally sequenced for 363 individuals belonging to 70 species in 38 genera. All specimen provenance data and associated sequence information were recorded in the barcode of life data systems (BOLD; www.barcodinglife.org ). Analytical tools on BOLD were used to assess the performance of barcoding to identify species. Using neighbor-joining distance comparison, the average genetic distance was 60-fold higher between species than within species, as pairwise genetic distance estimates averaged 10.29% among congeners and only 0.17% among conspecifics. Despite low levels of divergence within species, we observed river system-specific haplotype partitioning within eight species (11.4% of all species). Our preliminary results suggest that DNA barcoding is very effective for species identification of Nigerian freshwater fishes.

  8. Net ground speed of downstream migrating radio-tagged Atlantic salmon ( Salmo salar L.) and brown trout ( Salmo trutta L.) smolts in relation to environmental factors

    DEFF Research Database (Denmark)

    Aarestrup, Kim; Nielsen, C.; Koed, Anders

    2002-01-01

    tagged and released in the Danish River Lilleaa. The downstream migration of the different groups of fish was monitored by manual tracking and by three automatic listening stations. The downstream migration of radio tagged smolts of both species occurred concurrently with their untagged counterparts....... The diel migration pattern of the radio tagged smolts was predominantly nocturnal in both species. Wild sea trout smolt migrated significantly faster than both the F1 trout and the introduced salmon. There was no correlation between net ground speed, gill Na+, K+-ATPase activity or fish length in any...

  9. A barrier to upstream migration in the fish passage of Itaipu Dam (Canal da Piracema), Paraná River basin

    Science.gov (United States)

    ,; Fontes Júnior, Hélio Martins; Makrakis, Sergio; Gomes, Luiz Carlos; Latini, João Dirço

    2012-01-01

    The majority of the fish passages built in the Neotropical region are characterised by low efficiency and high selectivity; in many cases, the benefits to fish populations are uncertain. Studies conducted in the Canal da Piracema at Itaipu dam on the Parana River indicate that the system component designated as the Discharge channel in the Bela Vista River (herein named Canal de deságue no rio Bela Vista or CABV), a 200 m long technical section, was the main barrier to the upstream migration. The aim of this study was to evaluate the degree of restriction imposed by the CABV on upstream movements of Prochilodus lineatus and Leporinus elongatus, Characiformes. Fish were tagged with passive integrated transponders (PIT tags) and released both downstream and upstream of this critical section. Individuals of both species released downstream of the CABV took much more time to reach the upper end of the system (43.6 days vs. 15.9 days), and passed in much lower proportions (18% vs. 60.8%) than those tagged upstream of this component. Although more work is needed to differentiate between fishway effects and natural variation in migratory motivation, the results clearly demonstrate passage problems at the CABV.

  10. Blue tilapia (Oreochromis aureus) predation on fishes in the Muddy River system, Clark County, Nevada

    Science.gov (United States)

    Scoppettone, G.G.; Salgado, J.A.; Nielsen, M.B.

    2005-01-01

    Blue tilapia (Oreochromis aureus), native to North Africa and the Middle East (Courtenay and Robins 1973, Fuller et al. 1999), has been introduced around the world as a human food source, for vegetation control, and as a game fish (Costa-Pierce and Riedel 2000). Blue tilapia has been particularly successful in establishing and spreading in North American waters where it has been reported to change fish community structure and cause native fish decline (Courtenay and Robins 1973, Fuller et al. 1999). Because of these detrimental effects, it is now generally considered an unwelcome introduction into North American waters (Dill and Cordone 1997, Fuller et al. 1999).

  11. A novel liposome-based nanocarrier loaded with an LPS-dsRNA cocktail for fish innate immune system stimulation.

    Directory of Open Access Journals (Sweden)

    Angels Ruyra

    Full Text Available Development of novel systems of vaccine delivery is a growing demand of the aquaculture industry. Nano- and micro- encapsulation systems are promising tools to achieve efficient vaccines against orphan vaccine fish diseases. In this context, the use of liposomal based-nanocarriers has been poorly explored in fish; although liposomal nanocarriers have successfully been used in other species. Here, we report a new ∼125 nm-in-diameter unilamellar liposome-encapsulated immunostimulant cocktail containing crude lipopolysaccharide (LPS from E. coli and polyinosinic:polycytidylic acid [poly (I:C], a synthetic analog of dsRNA virus, aiming to be used as a non-specific vaccine nanocarrier in different fish species. This liposomal carrier showed high encapsulation efficiencies and low toxicity not only in vitro using three different cellular models but also in vivo using zebrafish embryos and larvae. We showed that such liposomal LPS-dsRNA cocktail is able to enter into contact with zebrafish hepatocytes (ZFL cell line and trout macrophage plasma membranes, being preferentially internalized through caveolae-dependent endocytosis, although clathrin-mediated endocytosis in ZFL cells and macropinocytocis in macrophages also contribute to liposome uptake. Importantly, we also demonstrated that this liposomal LPS-dsRNA cocktail elicits a specific pro-inflammatory and anti-viral response in both zebrafish hepatocytes and trout macrophages. The design of a unique delivery system with the ability to stimulate two potent innate immunity pathways virtually present in all fish species represents a completely new approach in fish health.

  12. Hypergraph topological quantities for tagged social networks

    Science.gov (United States)

    Zlatić, Vinko; Ghoshal, Gourab; Caldarelli, Guido

    2009-09-01

    Recent years have witnessed the emergence of a new class of social networks, which require us to move beyond previously employed representations of complex graph structures. A notable example is that of the folksonomy, an online process where users collaboratively employ tags to resources to impart structure to an otherwise undifferentiated database. In a recent paper, we proposed a mathematical model that represents these structures as tripartite hypergraphs and defined basic topological quantities of interest. In this paper, we extend our model by defining additional quantities such as edge distributions, vertex similarity and correlations as well as clustering. We then empirically measure these quantities on two real life folksonomies, the popular online photo sharing site Flickr and the bookmarking site CiteULike. We find that these systems share similar qualitative features with the majority of complex networks that have been previously studied. We propose that the quantities and methodology described here can be used as a standard tool in measuring the structure of tagged networks.

  13. Fish allergy: in review.

    Science.gov (United States)

    Sharp, Michael F; Lopata, Andreas L

    2014-06-01

    Globally, the rising consumption of fish and its derivatives, due to its nutritional value and divergence of international cuisines, has led to an increase in reports of adverse reactions to fish. Reactions to fish are not only mediated by the immune system causing allergies, but are often caused by various toxins and parasites including ciguatera and Anisakis. Allergic reactions to fish can be serious and life threatening and children usually do not outgrow this type of food allergy. The route of exposure is not only restricted to ingestion but include manual handling and inhalation of cooking vapors in the domestic and occupational environment. Prevalence rates of self-reported fish allergy range from 0.2 to 2.29 % in the general population, but can reach up to 8 % among fish processing workers. Fish allergy seems to vary with geographical eating habits, type of fish processing, and fish species exposure. The major fish allergen characterized is parvalbumin in addition to several less well-known allergens. This contemporary review discusses interesting and new findings in the area of fish allergy including demographics, novel allergens identified, immunological mechanisms of sensitization, and innovative approaches in diagnosing and managing this life-long disease.

  14. Analysis of integrated animal-fish production system under subtropical hill agro ecosystem in India: growth performance of animals, total biomass production and monetary benefit.

    Science.gov (United States)

    Kumaresan, A; Pathak, K A; Bujarbaruah, K M; Vinod, K

    2009-03-01

    The present study assessed the benefits of integration of animals with fish production in optimizing the bio mass production from unit land in subtropical hill agro ecosystem. Hampshire pigs and Khaki Campbell ducks were integrated with composite fish culture. The pig and duck excreta were directly allowed into the pond and no supplementary feed was given to fish during the period of study. The average levels of N, P and K in dried pig and duck manure were 0.9, 0.7 and 0.6 per cent and 1.3, 0.6 and 0.5 per cent, respectively. The average body weight of pig and duck at 11 months age was 90 and 1.74 kg with an average daily weight gain of 333.33 and 6.44 g, respectively. The fish production in pig-fish and duck-fish systems were 2209 and 2964 kg/ha, respectively while the fish productivity in control pond was only 820 kg/ha. The total biomass (animal and fish) production was higher (pfeeding system compared to the traditional system, however the input/output ratio was 1:1.2 and 1:1.55 for commercial and traditional systems, respectively. It was inferred that the total biomass production per unit land was high (pfish were integrated together.

  15. The bremsstrahlung tagged photon beam in Hall B at JLab

    CERN Document Server

    Sober, D I; Longhi, A; Matthews, S K; O'Brien, J T; Berman, B L; Briscoe, W J; Cole, P L; Connelly, J P; Dodge, W R; Murphy, L Y; Philips, S A; Dugger, M K; Lawrence, D; Ritchie, B G; Smith, E S; Lambert, J M; Anciant, E; Audit, G; Auger, T; Marchand, C; Klusman, M; Napolitano, J; Khandaker, M A; Salgado, C W; Sarty, A J

    2000-01-01

    We describe the design and commissioning of the photon tagging beamline installed in experimental Hall B at the Thomas Jefferson National Accelerator Facility (JLab). This system can tag photon energies over a range from 20% to 95% of the incident electron energy, and is capable of operation with beam energies up to 6.1 GeV. A single dipole magnet is combined with a hodoscope containing two planar arrays of plastic scintillators to detect energy-degraded electrons from a thin bremsstrahlung radiator. The first layer of 384 partially overlapping small scintillators provides photon energy resolution, while the second layer of 61 larger scintillators provides the timing resolution necessary to form a coincidence with the corresponding nuclear interaction triggered by the tagged photon. The definitions of overlap channels in the first counter plane and of geometric correlation between the two planes are determined using digitized time information from the individual counters. Auxiliary beamline devices are briefl...

  16. Performance of the ALICE secondary vertex b-tagging algorithm

    CERN Document Server

    INSPIRE-00262232

    2016-11-04

    The identification of jets originating from beauty quarks in heavy-ion collisions is important to study the properties of the hot and dense matter produced in such collisions. A variety of algorithms for b-jet tagging was elaborated at the LHC experiments. They rely on the properties of B hadrons, i.e. their long lifetime, large mass and large multiplicity of decay products. In this work, the b-tagging algorithm based on displaced secondary-vertex topologies is described. We present Monte Carlo based performance studies of the algorithm for charged jets reconstructed with the ALICE tracking system in p-Pb collisions at $\\sqrt{s_\\text{NN}}$ = 5.02 TeV. The tagging efficiency, rejection rate and the correction of the smearing effects of non-ideal detector response are presented.

  17. Effect of heat treatment and packaging systems on the stability of fish sausage

    Directory of Open Access Journals (Sweden)

    Bruna Rafaela Dallabona

    2013-12-01

    Full Text Available The purpose of this study was to evaluate the physicochemical and microbiological stability of sausages produced from mechanically separated fish meat (MSM obtained from Nile tilapia filleting residues. Different heat treatments (pasteurization or smoking and packaging systems (conventional or vacuum were used. The sausages were characterized for chemical composition, weight loss, water activity, instrumental texture and sensorial analysis. Additionally, microbiological analysis, instrumental color, pH, thiobarbituric acid reactive substances (TBARS and total volatile base nitrogen (TVB-N were assessed during storage. No presence of Escherichia coli, Salmonella sp. or coagulase-positive Staphylococcus was detected; however, the psychrotrophic count in pasteurized sausages exceeded the limits allowed for consumption. pH and lipid oxidation speed (TBARS values were reduced when vacuum packaging was used. Volatile nitrogenous bases remained virtually constant during the storage period, and higher values were observed in smoked products. Pasteurized sausages remain stable for 10 and 15 days in conventional and vacuum packages, respectively, and smoked sausages remain stable for 25 and 45 days in conventional and vacuum packages, respectively.

  18. Performance of Encounternet Tags: Field Tests of Miniaturized Proximity Loggers for Use on Small Birds

    Science.gov (United States)

    Levin, Iris I.; Zonana, David M.; Burt, John M.; Safran, Rebecca J.

    2015-01-01

    Proximity logging is a new tool for understanding social behavior as it allows for accurate quantification of social networks. We report results from field calibration and deployment tests of miniaturized proximity tags (Encounternet), digital transceivers that log encounters between tagged individuals. We examined radio signal behavior in relation to tag attachment (tag, tag on bird, tag on saline-filled balloon) to understand how radio signal strength is affected by the tag mounting technique used for calibration tests. We investigated inter-tag and inter-receiver station variability, and in each calibration test we accounted for the effects of antennae orientation. Additionally, we used data from a live deployment on breeding barn swallows (Hirundo rustica erythrogaster) to analyze the quality of the logs, including reciprocal agreement in dyadic logs. We evaluated the impact (in terms of mass changes) of tag attachment on the birds. We were able to statistically distinguish between RSSI values associated with different close-proximity (station variability. Reciprocal agreement of dyadic logs was high and social networks were constructed from proximity tag logs based on two different RSSI thresholds. There was no evidence of significant mass loss in the time birds were wearing tags. We conclude that proximity loggers are accurate and effective for quantifying social behavior. However, because RSSI and distance cannot be perfectly resolved, data from proximity loggers are most appropriate for comparing networks based on specific RSSI thresholds. The Encounternet system is flexible and customizable, and tags are now light enough for use on small animals (<50g). PMID:26348329

  19. Distribution of mesopredatory fish determined by habitat variables in a predator-depleted coastal system

    OpenAIRE

    Bergstr?m, Lena; Karlsson, Martin; Bergstr?m, Ulf; Pihl, Leif; Kraufvelin, Patrik

    2016-01-01

    Shallow nearshore habitats are highly valued for supporting marine ecosystems, but are subject to intense human-induced pressures. Mesopredatory fish are key components in coastal food webs, and alterations in their abundance may have evident effects also on other parts of the ecosystem. The aim of this study was to clarify the relationship between the abundance of coastal mesopredatory fish, defined as mid-trophic level demersal and benthic species with a diet consisting predominantly of inv...

  20. Functional morphology of the luminescence system of Siphamia versicolor (Perciformes: Apogonidae), a bacterially luminous coral reef fish.

    Science.gov (United States)

    Dunlap, Paul V; Nakamura, Masaru

    2011-08-01

    Previous studies of the luminescence system of Siphamia versicolor (Perciformes: Apogonidae) identified a ventral light organ, reflector, lens, duct, and a ventral diffuser extending from the throat to the caudal peduncle. The control and function of luminescence in this and other species of Siphamia, however, have not been defined. Morphological examination of fresh and preserved specimens identified additional components of the luminescence system involved in control and ventral emission of luminescence, including a retractable shutter over the ventral face of the light organ, contiguity of the ventral diffuser from the caudal peduncle to near the chin, and transparency of the bones and other tissues of the lower jaw. The shutter halves retract laterally, allowing the ventral release of light, and relax medially, blocking ventral light emission; topical application of norepinephrine to the exposed light organ resulted in retraction of the shutter halves, which suggests that operation of the shutter is under neuromuscular control. The extension of the diffuser to near the chin and transparency of the lower jaw allow a uniform emission of luminescence over the entire ventrum of the fish. The live aquarium-held fish were found to readily and consistently display ventral luminescence. At twilight, the fish left the protective association with their longspine sea urchin, Diadema setosum, and began to emit ventral luminescence and to feed on zooplankton. Ventral luminescence illuminated a zone below and around the fish, which typically swam close to the substrate. Shortly after complete darkness, the fish stopped feeding and emitting luminescence. These observations suggest that S. versicolor uses ventral luminescence to attract and feed on zooplankton from the reef benthos at twilight. Ventral luminescence may allow S. versicolor to exploit for feeding the gap at twilight in the presence of potential predators as the reef transitions from diurnally active to

  1. Preliminary evaluation of a fluorescence and radioisotope nuclear smuggling deterrence tag - final report (IL500E)

    International Nuclear Information System (INIS)

    Hartenstein, S.D.; Aryaeinejad, R.; Delmastro, J.R.

    1997-04-01

    This report summarizes the efforts completed in identifying candidate fluorescence compounds and radioisotopes for a developing tagging system. The tagging system is being developed as a deterrent to nuclear smuggling, by providing a means of: (1) tracing materials and pilferers to the facility of origin for any recovered special nuclear materials, (2) inventory control of long-term stored items containing special nuclear materials, and (3) tracking materials transferred between facilities. The system uses three types of materials to cover a range of applications intended to prevent the pilfering of special nuclear materials. One material, fluorescent compounds which are invisible without ultraviolet or near-infrared detection systems, is marked on controlled items with a tracking pattern that corresponds to a specified item in a specified location in the data control system. The tagging system uses an invisible, fluorescent dusting powder to mark equipment and personnel who inappropriately handle the tagged material. The tagging system also uses unique combinations of radionuclides to identify the facility of origin for any special nuclear material. This report also summarizes the efforts completed in identifying hardware that will be used for the tagging system. This hardware includes the devices for applying the tagging materials, the commercially available fluorescence detection systems, and gamma ray detection systems assembled from existing, commercially available technologies

  2. Preliminary evaluation of a fluorescence and radioisotope nuclear smuggling deterrence tag - final report (IL500E)

    Energy Technology Data Exchange (ETDEWEB)

    Hartenstein, S.D.; Aryaeinejad, R.; Delmastro, J.R. [and others

    1997-04-01

    This report summarizes the efforts completed in identifying candidate fluorescence compounds and radioisotopes for a developing tagging system. The tagging system is being developed as a deterrent to nuclear smuggling, by providing a means of: (1) tracing materials and pilferers to the facility of origin for any recovered special nuclear materials, (2) inventory control of long-term stored items containing special nuclear materials, and (3) tracking materials transferred between facilities. The system uses three types of materials to cover a range of applications intended to prevent the pilfering of special nuclear materials. One material, fluorescent compounds which are invisible without ultraviolet or near-infrared detection systems, is marked on controlled items with a tracking pattern that corresponds to a specified item in a specified location in the data control system. The tagging system uses an invisible, fluorescent dusting powder to mark equipment and personnel who inappropriately handle the tagged material. The tagging system also uses unique combinations of radionuclides to identify the facility of origin for any special nuclear material. This report also summarizes the efforts completed in identifying hardware that will be used for the tagging system. This hardware includes the devices for applying the tagging materials, the commercially available fluorescence detection systems, and gamma ray detection systems assembled from existing, commercially available technologies.

  3. The effects of polycyclic aromatic hydrocarbons on the immune system of fish: A review

    International Nuclear Information System (INIS)

    Reynaud, S.; Deschaux, P.

    2006-01-01

    Polycyclic aromatic hydrocarbons are an important class of environmental pollutants that are known to be carcinogenic and immunotoxic. This review summarizes the diverse literature on the effects of these pollutants on innate and acquired immunity in fish and the mechanism of PAH-induced immunotoxicity. Among innate immune parameters, many authors have focused on macrophage activities in fish exposed to polycyclic aromatic hydrocarbons. Macrophage respiratory burst appears especially sensitive to polycyclic aromatic hydrocarbons. Among acquired immune parameters, lymphocyte proliferation appears highly sensitive to polycyclic aromatic hydrocarbon exposure. However, the effects of polycyclic aromatic hydrocarbons on both specific and non-specific immunity are contradictory and depend on the mode of exposure, the dose used or the species studied. In contrast to mammals, fewer studies have been done in fish to determine the mechanism of polycyclic aromatic hydrocarbon-induced toxicity. This phenomenon seems to implicate different intracellular mechanisms such as metabolism by cytochrome P4501A, binding to the Ah-receptor, or increased intracellular calcium. Advances in basic knowledge of fish immunity should lead to improvements in monitoring fish health and predicting the impact of polycyclic aromatic hydrocarbons on fish populations, which is a fundamental ecotoxicological goal

  4. Diet variability of forage fishes in the Northern California Current System

    Science.gov (United States)

    Hill, Andrew D.; Daly, Elizabeth A.; Brodeur, Richard D.

    2015-06-01

    As fisheries management shifts to an ecosystem-based approach, understanding energy pathways and trophic relationships in the Northern California Current (NCC) will become increasingly important for predictive modeling and understanding ecosystem response to changing ocean conditions. In the NCC, pelagic forage fishes are a critical link between seasonal and interannual variation in primary production and upper trophic groups. We compared diets among dominant forage fish (sardines, anchovies, herring, and smelts) in the NCC collected in May and June of 2011 and June 2012, and found high diet variability between and within species on seasonal and annual time scales, and also on decadal scales when compared to results of past studies conducted in the early 2000s. Copepoda were a large proportion by weight of several forage fish diets in 2011 and 2012, which differed from a preponderance of Euphausiidae found in previous studies, even though all years exhibited cool ocean conditions. We also examined diet overlap among these species and with co-occurring subyearling Chinook salmon and found that surf smelt diets overlapped more with subyearling Chinook diets than any other forage fish. Herring and sardine diets overlapped the most with each other in our interdecadal comparisons and some prey items were common to all forage fish diets. Forage fish that show plasticity in diet may be more adapted to ocean conditions of low productivity or anomalous prey fields. These findings highlight the variable and not well-understood connections between ocean conditions and energy pathways within the NCC.

  5. The effects of polycyclic aromatic hydrocarbons on the immune system of fish: A review

    Energy Technology Data Exchange (ETDEWEB)

    Reynaud, S. [Laboratoire d' Ecologie Alpine. UMR CNRS 5553. Universite Joseph Fourier. BP 53. 38041 Grenoble cedex 9 (France) and Laboratory of General and Comparative Immunophysiology, Science Teaching and Research Unit, 123, av. Albert Thomas, 87060 Limoges (France)]. E-mail: stephane.reynaud@ujf-grenoble.fr; Deschaux, P. [Laboratory of General and Comparative Immunophysiology, Science Teaching and Research Unit, 123, av. Albert Thomas, 87060 Limoges (France)

    2006-05-01

    Polycyclic aromatic hydrocarbons are an important class of environmental pollutants that are known to be carcinogenic and immunotoxic. This review summarizes the diverse literature on the effects of these pollutants on innate and acquired immunity in fish and the mechanism of PAH-induced immunotoxicity. Among innate immune parameters, many authors have focused on macrophage activities in fish exposed to polycyclic aromatic hydrocarbons. Macrophage respiratory burst appears especially sensitive to polycyclic aromatic hydrocarbons. Among acquired immune parameters, lymphocyte proliferation appears highly sensitive to polycyclic aromatic hydrocarbon exposure. However, the effects of polycyclic aromatic hydrocarbons on both specific and non-specific immunity are contradictory and depend on the mode of exposure, the dose used or the species studied. In contrast to mammals, fewer studies have been done in fish to determine the mechanism of polycyclic aromatic hydrocarbon-induced toxicity. This phenomenon seems to implicate different intracellular mechanisms such as metabolism by cytochrome P4501A, binding to the Ah-receptor, or increased intracellular calcium. Advances in basic knowledge of fish immunity should lead to improvements in monitoring fish health and predicting the impact of polycyclic aromatic hydrocarbons on fish populations, which is a fundamental ecotoxicological goal.

  6. Coping with flow: behavior, neurophysiology and modeling of the fish lateral line system.

    Science.gov (United States)

    Mogdans, Joachim; Bleckmann, Horst

    2012-12-01

    With the mechanosensory lateral line fish perceive water motions relative to their body surface and local pressure gradients. The lateral line plays an important role in many fish behaviors including the detection and localization of dipole sources and the tracking of prey fish. The sensory units of the lateral line are the neuromasts which are distributed across the surface of the animal. Water motions are received and transduced into neuronal signals by the neuromasts. These signals are conveyed by afferent nerve fibers to the fish brain and processed by lateral line neurons in parts of the brainstem, cerebellum, midbrain, and forebrain. In the cerebellum, midbrain, and forebrain, lateral line information is integrated with sensory information from other modalities. The present review introduces the peripheral morphology of the lateral line, and describes our understanding of lateral line physiology and behavior. It focuses on recent studies that have investigated: how fish behave in unsteady flow; what kind of sensory information is provided by flow; and how fish use and process this information. Finally, it reports new theoretical and biomimetic approaches to understand lateral line function.

  7. Arabic medical entity tagging using distant learning

    Directory of Open Access Journals (Sweden)

    Viviana Cotik

    2017-04-01

    Full Text Available A semantic tagger aiming to detect relevant entities in Arabic medical documents and tagging them with their appropriate semantic class is presented. The system takes profit of a Multilingual Framework covering four languages (Arabic, English, French, and Spanish, in a way that resources available for each language can be used to improve the results of the others, this is specially important for less resourced languages as Arabic. The approach has been evaluated against Wikipedia pages of the four languages belonging to the medical domain. The core of the system is the definition of a base tagset consisting of the three most represented classes in SNOMED-CT taxonomy and the learning of a binary classifier for each semantic category in the tagset and each language, using a distant learning approach over three widely used knowledge resources, namely Wikipedia, Dbpedia, and SNOMED-CT.

  8. Fish and hydroelectricity

    International Nuclear Information System (INIS)

    Zorpette, G.

    1990-01-01

    This paper reports on the problems that hydroelectric plants have regarding fish populations. The utilities that operate these plants are finding that accommodating migrating fish presents unique engineering challenges, not the least of which involves designing and building systems to protect fish species whose migratory behavior remains something of a mystery. Where such systems cannot be built, the status of hydroelectric dams may be in doubt, as is now the case with several dams in the United States. A further twist in some regions in the possibility that certain migratory fish will be declared threatened or endangered-a development that could wreak havoc on the hydroelectric energy supply in those regions

  9. Building Tag Clouds in Perl and PHP

    CERN Document Server

    Bumgardner, Jim

    2006-01-01

    Tag clouds are everywhere on the web these days. First popularized by the web sites Flickr, Technorati, and del.icio.us, these amorphous clumps of words now appear on a slew of web sites as visual evidence of their membership in the elite corps of "Web 2.0." This PDF analyzes what is and isn't a tag cloud, offers design tips for using them effectively, and then goes on to show how to collect tags and display them in the tag cloud format. Scripts are provided in Perl and PHP. Yes, some have said tag clouds are a fad. But as you will see, tag clouds, when used properly, have real merits. More

  10. Assessment of the effects of fish meal, wheat gluten, soy protein concentrate and feed moisture on extruder system parameters and the technical quality of fish feed

    NARCIS (Netherlands)

    Draganovic, V.; Goot, van der A.J.; Boom, R.M.; Jonkers, J.

    2011-01-01

    Evaluation of feed ingredient functionality plays a vital role in modern fish feed manufacturing practice. The aim of this study was to examine the extrusion behaviour of blends containing alternative protein sources from plant origin to fish meal (FM), such as wheat gluten (WG) and soy protein

  11. A Multimodal Database for Affect Recognition and Implicit Tagging

    NARCIS (Netherlands)

    Soleymani, Mohammad; Lichtenauer, Jeroen; Pun, Thierry; Pantic, Maja

    MAHNOB-HCI is a multimodal database recorded in response to affective stimuli with the goal of emotion recognition and implicit tagging research. A multimodal setup was arranged for synchronized recording of face videos, audio signals, eye gaze data, and peripheral/central nervous system

  12. LHCb New algorithms for Flavour Tagging at the LHCb experiment

    CERN Multimedia

    Fazzini, Davide

    2016-01-01

    The Flavour Tagging technique allows to identify the B initial flavour, required in the measurements of flavour oscillations and time-dependent CP asymmetries in neutral B meson systems. The identification performances at LHCb are further enhanced thanks to the contribution of new algorithms.

  13. Learner Corpora without Error Tagging

    Directory of Open Access Journals (Sweden)

    Rastelli, Stefano

    2009-01-01

    Full Text Available The article explores the possibility of adopting a form-to-function perspective when annotating learner corpora in order to get deeper insights about systematic features of interlanguage. A split between forms and functions (or categories is desirable in order to avoid the "comparative fallacy" and because – especially in basic varieties – forms may precede functions (e.g., what resembles to a "noun" might have a different function or a function may show up in unexpected forms. In the computer-aided error analysis tradition, all items produced by learners are traced to a grid of error tags which is based on the categories of the target language. Differently, we believe it is possible to record and make retrievable both words and sequence of characters independently from their functional-grammatical label in the target language. For this purpose at the University of Pavia we adapted a probabilistic POS tagger designed for L1 on L2 data. Despite the criticism that this operation can raise, we found that it is better to work with "virtual categories" rather than with errors. The article outlines the theoretical background of the project and shows some examples in which some potential of SLA-oriented (non error-based tagging will be possibly made clearer.

  14. B-tagging in CMS at LHC

    CERN Document Server

    Cucciarelli, S

    2003-01-01

    This report provides a review of the main algorithms for offline inclusive b-tagging developed within the CMS community. Two b-tag algorithms, one based on the impact parameter measurement and the other based on the secondary vertices are discussed. The performance of these algorithms are presented for several jet transverse energies and pseudorapidity regions. An additional decay length based b-tag is also described and its preliminary performance is presented. (4 refs) .

  15. Fish Allergy

    Science.gov (United States)

    ... Safe Videos for Educators Search English Español Fish Allergy KidsHealth / For Parents / Fish Allergy What's in this ... Print en español Alergia al pescado About Fish Allergy A fish allergy is not exactly the same ...

  16. Developing a System for Fish Fauna Migration Restoration Above the Spillway Sill Near the City Hall of Oradea (Romania

    Directory of Open Access Journals (Sweden)

    Voicu Răzvan

    2015-12-01

    Full Text Available The channel of the river Crișul Repede inside the town affects both the biodiversity and implicit functionality of Crișul Repede River, therefore, this article aims to provide a solution for fish fauna migration in a system designed to restore longitudinal connectivity. The proposed migration system is based on the gravitational fall of water and will lead to the restoration of the longitudinal connection of Crişul Repede River near the weir selected as the study case. It will reconnect approximately three kilometers of habitat that will contribute to ensuring the optimal conditions for the development of migratory fish species present in the area.

  17. The use of IFIM for evaluating effects of a flow alternative on fish habitat in a river system with competing water demands

    International Nuclear Information System (INIS)

    Miller, W.J.; Chadwick, J.W.; Canton, S.P.; Conklin, D.J. Jr.; Chrisp, E.Y.

    1991-01-01

    This paper reports on the Instream Flow Incremental Methodology (IFIM) which was used to evaluate instream fish habitat in the Platte River in central Nebraska. The IFIM analysis presented herein incorporates water temperature modeling and water quality, fish species composition and distribution, physical habitat data and 43 years of flow records. The Platte River system has competing water demands from hydropower, agricultural irrigation, municipal uses, recreation and most recently from recommended instream flows for fish and wildlife resources. IFIM was the tool used to develop the data base required for a comprehensive instream flow analysis of the system. When compared to the baseline flow regime, and alternative flow regime significantly increased modelled fish habitat area during critical periods of the year. The time series results demonstrated that the flow alternative would be beneficial to the existing fish resources, while still providing water for power production and irrigation

  18. Graph based techniques for tag cloud generation

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2013-01-01

    Tag cloud is one of the navigation aids for exploring documents. Tag cloud also link documents through the user defined terms. We explore various graph based techniques to improve the tag cloud generation. Moreover, we introduce relevance measures based on underlying data such as ratings...... or citation counts for improved measurement of relevance of tag clouds. We show, that on the given data sets, our approach outperforms the state of the art baseline methods with respect to such relevance by 41 % on Movielens dataset and by 11 % on Bibsonomy data set....

  19. Flavour Tagging developments within the LHCb experiment

    CERN Document Server

    Grabalosa, Marc

    Flavour Tagging at the LHCb experiment is a fundamental tool for the measurement of B oscillations and the study of CP violation. This document explains the development of different tagging techniques and the different strategies used to combine them to determine the flavour of the B meson as precisely as possible. The response of the tagging algorithms also needs to be optimized and calibrated. Both procedures are described using the available LHCb datasets corresponding to various integrated luminosities. First results on the tagging performances are shown for different control channels and physics measurements.

  20. Using Interference to Block RFID Tags

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund

    We propose a novel method to block RFID tags from responding, using intentional interference. We focus on the experimental evaluation, where we impose interference on the download and uplink, respectively. The results are positive, where modulated CCI shows most effective to block a tag.......We propose a novel method to block RFID tags from responding, using intentional interference. We focus on the experimental evaluation, where we impose interference on the download and uplink, respectively. The results are positive, where modulated CCI shows most effective to block a tag....