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Sample records for file bl stn

  1. Safety Evaluation Report related to the operation of Palo Verde Nuclear Generating Station, Units 1, 2, and 3 (Docket Nos. STN 50-528, STN 50-529, and STN 50-530)

    International Nuclear Information System (INIS)

    1984-10-01

    Supplement No. 6 to the Safety Evaluation Report for the application filed by Arizona Public Service Company, et al., for licenses to operate the Palo Verde Nuclear Generating Station, Units 1, 2, and 3 (Docket Nos. STN 50-528/529/530), located in Maricopa County, Arizona, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation Report by providing an evaluation of (1) additional information submitted by the applicant since Supplement No. 5 was issued and (2) matters that the staff had under review when Supplement No. 5 was issued

  2. Safety Evaluation Report related to the operation of Palo Verde Nuclear Generating Station, Units 1, 2, and 3 (Docket Nos. STN 50-528, STN 50-529, and STN 50-530). Supplement No. 7

    International Nuclear Information System (INIS)

    1984-12-01

    Supplement No. 7 to the Safety Evaluation Report for the application filed by Arizona Public Service Company et al. for licenses to operate the Palo Verde Nuclear Generating Station, Units 1, 2, and 3 (Docket Nos. STN 50-528/529/530), located in Maricopa County, Arizona, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation Report by providing an evaluation of: (1) additional information submitted by the applicant since Supplement No. 6 was issued; and (2) matters that the staff had under review when Supplement No. 6 was issued

  3. ISLSCP II River Routing Data (STN-30p)

    Data.gov (United States)

    National Aeronautics and Space Administration — The Simulated Topological Network (STN-30p) data set provides the large-scale hydrological modeling community an accurate representation of the global river system...

  4. ISLSCP II River Routing Data (STN-30p)

    Data.gov (United States)

    National Aeronautics and Space Administration — ABSTRACT: The Simulated Topological Network (STN-30p) data set provides the large-scale hydrological modeling community an accurate representation of the global...

  5. Safety Evaluation Report related to the operation of Palo Verde Nuclear Generating Station, Units 1, 2, and 3 (Dockets Nos. STN 50-528, STN 50-529, and STN 50-530)

    International Nuclear Information System (INIS)

    1987-03-01

    Supplement No. 11 to the Safety Evaluation Report for the application filed by Arizona Public Service Company et al. for licenses to operate the Palo Verde Nuclear Generating Station, Units 1, 2, and 3 (Docket Nos. STN 50-528/529/530), located in Maricopa County, Arizone, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation Report by providing an evaluation of (1) additional information submitted by the applicant since Supplement No. 10 was issued and (2) other matters requiring staff review since Supplenent No. 10 was issued, specifically those issues that required resolution before Unit 3 low-power licensing

  6. Safety Evaluation Report related to the operation of Palo Verde Nuclear Generating Station, Units 1, 2, and 3 (Docket Nos. STN 50-528, STN 50-529, and STN 50-530). Supplement No. 9

    International Nuclear Information System (INIS)

    1985-12-01

    Supplement No. 9 to the Safety Evaluation Report for the application filed by Arizona Public Service Company et al. for licenses to operate the Palo Verde Nuclear Generating Station, Units 1, 2 and 3 (Docket Nos. STN 50-528/529/530), located in Maricopa County, Arizona, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation Report by providing an evaluation of: (1) additional information submitted by the applicant since Supplement No. 8 was issued; and (2) matters that the staff had under review when Supplement No. 8 was issued, specifically those issues which required resolution prior to Unit 2 fuel loading and testing up to 5% of full power

  7. Stimulation site within the MRI-defined STN predicts postoperative motor outcome.

    Science.gov (United States)

    Wodarg, Fritz; Herzog, Jan; Reese, René; Falk, Daniela; Pinsker, Markus O; Steigerwald, Frank; Jansen, Olav; Deuschl, Günther; Mehdorn, H Maximillian; Volkmann, Jens

    2012-06-01

    High-frequency stimulation of the subthalamic nucleus (STN-HFS) is highly effective in treating motor symptoms in Parkinson's disease (PD) and medication side effects as well as in improving quality of life. Despite preoperative screening for patients as eligible candidates for this treatment, electrode position may furthermore influence treatment quality. Here, we investigated the relationship between the anatomical site of stimulation within the MRI-defined STN and the outcome of PD patients after STN-HFS. In 30 PD patients with bilateral STN stimulation, we retrospectively defined the boundaries of the STN within the axial target plane of the stereotactic T2-weighted MRI and determined the position of the active electrode contact in relation to the border of the STN. The position of the active contact within the STN was the only variable to predict the outcome of STN stimulation. In contrast, covariates such as age, disease duration, symptom severity, and response to levodopa had no effect. The lateral position of the stimulation contact within the STN led to significantly better clinical improvement, lower stimulation parameters, and less need for postoperative dopaminergic medication. The outcome of patients with stimulation contacts within the medial region of the STN was significantly worse. Precise targeting of the lateral region of the STN is essential for achieving sufficient stimulation efficacy. Preoperative T2-weighted MRI might be a useful component of the targeting procedure to improve the outcome of PD patients. Copyright © 2012 Movement Disorder Society.

  8. Comparison of weight changes following unilateral and staged bilateral STN DBS for advanced PD.

    Science.gov (United States)

    Lee, Eric M; Kurundkar, Ashish; Cutter, Gary R; Huang, He; Guthrie, Barton L; Watts, Ray L; Walker, Harrison C

    2011-09-01

    Unilateral and bilateral subthalamic nucleus deep brain stimulation (STN DBS) in Parkinson's disease (PD) result in weight gain in the initial postoperative months, but little is known about the changes in weight following unilateral and staged bilateral STN DBS over longer time intervals. A case-control comparison evaluated weight changes over 2 years in 43 consecutive unilateral STN DBS patients, among whom 25 elected to undergo staged bilateral STN DBS, and 21 age-matched and disease severity matched PD controls without DBS. Regression analyses incorporating age, gender, and baseline weight in case or control were conducted to assess weight changes 2 years after the initial unilateral surgery. Unilateral STN DBS and staged bilateral STN DBS patients gained 3.9 ± 2.0 kg and 5.6 ± 2.1 kg versus their preoperative baseline weight (P < 0.001, respectively) while PD controls without DBS lost 0.8 ± 1.1 kg. Although bilateral STN DBS patients gained 1.7 kg more than unilateral STN DBS patients at 2 years, this difference was not statistically significant (P = 0.885). Although there was a trend toward greater weight gain in staged bilateral STN DBS patients versus unilateral patients, we found no evidence for an equivalent or synergistic increase in body weight following placement of the second DBS electrode.

  9. Status report on nuclear power - information from STN databases

    International Nuclear Information System (INIS)

    Prinz, H.

    1995-01-01

    The worldwide future of nuclear power as seen about 25 years ago is presented based on a literature search in the INIS database. The role of nuclear power today, after TMI and Chernobyl, in energy supplies and in combating the greehouse effect is evaluated by literature searches in STN databases (e.g. INIS, ETDE, COMPENDEX, CA, ULIDAT, INSPEC). An evaluation is given of the different information contents of bibliographic databases such as INIS and pure information databases such as NLDB. (orig./HP)

  10. Safety-evaluation report related to the final design of the Standard Nuclear Steam Supply Reference System - CESSAR System 80. Docket No. STN 50-470

    International Nuclear Information System (INIS)

    1983-03-01

    Supplement No. 1 to the Safety Evaluation Report for the application filed by Combustion Engineering, Inc. for a Final Design Approval for the Combustion Engineering Standard Safety Analysis Report (STN 50-470) has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation by providing: (1) the evaluation of additional information submitted by the applicant since the Safety Evaluation Report was issued, (2) the evaluation of the matters the staff had under review when the Safety Evaluation Report was issued, and (3) the response to comments made by the Advisory Committee on Reactor Safeguards

  11. Safety Evaluation Report related to the operation of Wolf Creek Generating Station, Unit No. 1 (Docket No. STN 50-482). Supplement No. 6

    International Nuclear Information System (INIS)

    1985-06-01

    This report supplements the Safety Evaluation Report (SER) for the application filed by the Kansas Gas and Electric Company, as applicant and agent for the owners, for a license to operate the Wolf Creek Generating Station, Unit 1 (Docket No. STN 50-482). The facility is located in Coffey County, Kansas. This supplement provides recent information regarding resolution of the license conditions identified in the SER. Because of the favorable resolution of the items discussed in this report, the staff concludes that the facility can be operated by the licensee at power levels greater than 5% without endangering the health and safety of the public

  12. STN-stimulation in Parkinson's disease restores striatal inhibition of thalamocortical projection

    DEFF Research Database (Denmark)

    Geday, Jacob; Østergaard, Karen; Johnsen, Erik

    2009-01-01

    To test the hypothesis that deep brain stimulation of the subthalamic nucleus (STN) restores the inhibitory output to the striatothalamocortical loop in Parkinson's disease, we obtained functional brain images of blood flow in 10 STN-stimulated patients with Parkinson's disease. Patients were...... in the STN and in the left nucleus lentiformis. Conversely, flow declined in the left supplementary motor area (BA 6), ventrolateral nucleus of the left thalamus, and right cerebellum. Activation of the basal ganglia and deactivation of supplementary motor area and thalamus were both correlated...... with the improvement of motor function. The result is consistent with the explanation that stimulation in resting patients raises output from the STN with activation of the inhibitory basal ganglia output nuclei and subsequent deactivation of the thalamic anteroventral and ventrolateral nuclei and the supplementary...

  13. BL Lacertae objects

    International Nuclear Information System (INIS)

    Miller, J.S.

    1978-01-01

    An overview is given of the principal characteristics and problems associated with the prototype BL Lacertae. The most important characteristics of this group and its relevance, the consideration of a few particular objects in moderate detail, the relation between these objects QSOs, and normal galaxies, and finally the possible physical nature of BL Lac objects and the important questions they raise are treated. 15 references

  14. Inhibition of Serratia marcescens Smj-11 biofilm formation by Alcaligenes faecalis STN17 crude extract

    International Nuclear Information System (INIS)

    Lutfi, Zainal; Ahmad, Asmat; Usup, Gires

    2014-01-01

    Serratia marcescens biofilms are formed when they are bound to surfaces in aqueous environments. S. marcescens utilizes N-acylhomoserine lactone (AHL) as its quorum sensing signal molecule. The accumulation of AHL indicates the bacteria to produce matrices to form biofilms. Prodigiosin (2-methyl-3-pentyl-6-methoxyprodigiosin), which causes red pigmentation in the colonies, are also produced when the AHL reaches a certain threshold. The Alcaligenes faecalis STN17 crude extract is believed to inhibit quorum sensing in the S. marcescens Smj-11 and, thus, impedes its biofilm formation ability. A. faecalis STN17 was grown in marine broth, and ethyl acetate extraction was carried out. The crude compound of A. faecalis STN17 was diluted at high concentration (0.2-6.4 mg/mL) and was taken to confirm anti-biofilm activity through the crystal violet method in 96-wells plate. Then, the crude extract underwent purification using simple solvents partitioning test to discern the respective compounds that had the anti-biofilm activity under the crystal violet method. The crystal violet test showed that the crude did have anti-biofilm activity on S. marcescens Smj-11, but did not kill the cells. This finding signifies that the suppression of biofilm formation in S. marcescens by A. faecalis STN17 has a strong correlation. The partitioning test showed that A. faecalis STN17 crude extract has several compounds and only the compound(s) in chloroform showed activities. In conclusion, the crude extract of A. faecalis STN17 has the ability to inhibit S. marcescens Smj-11 biofilm formation

  15. Inhibition of Serratia marcescens Smj-11 biofilm formation by Alcaligenes faecalis STN17 crude extract

    Energy Technology Data Exchange (ETDEWEB)

    Lutfi, Zainal; Ahmad, Asmat [School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor (Malaysia); Usup, Gires [School of Environmental and Natural Resources Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor (Malaysia)

    2014-09-03

    Serratia marcescens biofilms are formed when they are bound to surfaces in aqueous environments. S. marcescens utilizes N-acylhomoserine lactone (AHL) as its quorum sensing signal molecule. The accumulation of AHL indicates the bacteria to produce matrices to form biofilms. Prodigiosin (2-methyl-3-pentyl-6-methoxyprodigiosin), which causes red pigmentation in the colonies, are also produced when the AHL reaches a certain threshold. The Alcaligenes faecalis STN17 crude extract is believed to inhibit quorum sensing in the S. marcescens Smj-11 and, thus, impedes its biofilm formation ability. A. faecalis STN17 was grown in marine broth, and ethyl acetate extraction was carried out. The crude compound of A. faecalis STN17 was diluted at high concentration (0.2-6.4 mg/mL) and was taken to confirm anti-biofilm activity through the crystal violet method in 96-wells plate. Then, the crude extract underwent purification using simple solvents partitioning test to discern the respective compounds that had the anti-biofilm activity under the crystal violet method. The crystal violet test showed that the crude did have anti-biofilm activity on S. marcescens Smj-11, but did not kill the cells. This finding signifies that the suppression of biofilm formation in S. marcescens by A. faecalis STN17 has a strong correlation. The partitioning test showed that A. faecalis STN17 crude extract has several compounds and only the compound(s) in chloroform showed activities. In conclusion, the crude extract of A. faecalis STN17 has the ability to inhibit S. marcescens Smj-11 biofilm formation.

  16. BL Lacertae objects

    International Nuclear Information System (INIS)

    Disney, M.J.; Veron, P.

    1977-01-01

    The properties of BL Lacertae objects are discussed including their spectra, variability, and brightness. The historical development of observation, and the conclusion that these objects are possibly quasar-related objects rather than variable stars as originally supposed are treated. The possible mechanisms for the unusual luminosity of these objects are considered

  17. Neuropsychological effects of bilateral STN stimulation in Parkinson disease - A controlled study

    NARCIS (Netherlands)

    Smeding, HMM; Koning-Haanstra, M; Schuurman, PR; Nijssen, P; van Laar, T; Schmand, B; Speelman, J.D.

    2006-01-01

    Objective: To evaluate the cognitive and behavioral effects of bilateral subthalamic nucleus (STN) stimulation in patients with Parkinson disease (PD). Methods: The authors included 103 patients; 99 patients were evaluated 6 months after surgery. A control group of 39 patients with PD was formed and

  18. CTC1-STN1 coordinates G- and C-strand synthesis to regulate telomere length.

    Science.gov (United States)

    Gu, Peili; Jia, Shuting; Takasugi, Taylor; Smith, Eric; Nandakumar, Jayakrishnan; Hendrickson, Eric; Chang, Sandy

    2018-05-17

    Coats plus (CP) is a rare autosomal recessive disorder caused by mutations in CTC1, a component of the CST (CTC1, STN1, and TEN1) complex important for telomere length maintenance. The molecular basis of how CP mutations impact upon telomere length remains unclear. The CP CTC1 L1142H mutation has been previously shown to disrupt telomere maintenance. In this study, we used CRISPR/Cas9 to engineer this mutation into both alleles of HCT116 and RPE cells to demonstrate that CTC1:STN1 interaction is required to repress telomerase activity. CTC1 L1142H interacts poorly with STN1, leading to telomerase-mediated telomere elongation. Impaired interaction between CTC1 L1142H :STN1 and DNA Pol-α results in increased telomerase recruitment to telomeres and further telomere elongation, revealing that C:S binding to DNA Pol-α is required to fully repress telomerase activity. CP CTC1 mutants that fail to interact with DNA Pol-α resulted in loss of C-strand maintenance and catastrophic telomere shortening. Our findings place the CST complex as an important regulator of both G-strand extensions by telomerase and C-strand synthesis by DNA Pol-α. © 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

  19. Safety Evaluation Report related to the operation of Wolf Creek Generating Station, Unit No. 1 (Docket No. STN 50-482). Supplement No. 5

    International Nuclear Information System (INIS)

    1985-03-01

    This report supplements the Safety Evaluation Report (SER) for the application filed by the Kansas Gas and Electric Company, as applicant and agent for the owners, for a license to operate the Wolf Creek Generating Station, Unit 1 (Docket No. STN 50-482). The facility is located in Coffey County, Kansas. This supplement has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission and provides recent information regarding resolution of the open items identified in the SER. Because of the favorable resolution of the items discussed in this report, the staff concludes that the facility can be operated by the applicant without endangering the health and safety of the public

  20. Effects of STN and GPi deep brain stimulation on impulse control disorders and dopamine dysregulation syndrome.

    Directory of Open Access Journals (Sweden)

    Sarah J Moum

    Full Text Available Impulse control disorders (ICDs and dopamine dysregulation syndrome (DDS are important behavioral problems that affect a subpopulation of patients with Parkinson's disease (PD and typically result in markedly diminished quality of life for patients and their caregivers. We aimed to investigate the effects of subthalamic nucleus (STN and internal globus pallidus (GPi deep brain stimulation (DBS on ICD/DDS frequency and dopaminergic medication usage.A retrospective chart review was performed on 159 individuals who underwent unilateral or bilateral PD DBS surgery in either STN or GPi. According to published criteria, pre- and post-operative records were reviewed to categorize patients both pre- and post-operatively as having ICD, DDS, both ICD and DDS, or neither ICD nor DDS. Group differences in patient demographics, clinical presentations, levodopa equivalent dose (LED, and change in diagnosis following unilateral/bilateral by brain target (STN or GPi DBS placement were examined.28 patients met diagnostic criteria for ICD or DDS pre- or post-operatively. ICD or DDS classification did not differ by GPi or STN target stimulation. There was no change in DDS diagnosis after unilateral or bilateral stimulation. For ICD, diagnosis resolved in 2 of 7 individuals after unilateral or bilateral DBS. Post-operative development of these syndromes was significant; 17 patients developed ICD diagnoses post-operatively with 2 patients with pre-operative ICD developing DDS post-operatively.Unilateral or bilateral DBS did not significantly treat DDS or ICD in our sample, even though a few cases of ICD resolved post-operatively. Rather, our study provides preliminary evidence that DDS and ICD diagnoses may emerge following DBS surgery.

  1. Coordinated reset stimulation in a large-scale model of the STN-GPe circuit

    Directory of Open Access Journals (Sweden)

    Martin eEbert

    2014-11-01

    Full Text Available Synchronization of populations of neurons is a hallmark of several brain diseases. Coordinated reset (CR stimulation is a model-based stimulation technique which specifically counteracts abnormal synchrony by desynchronization. Electrical CR stimulation, e.g. for the treatment of Parkinson’s disease (PD, is administered via depth electrodes. In order to get a deeper understanding of this technique, we extended the top-down approach of previous studies and constructed a large-scale computational model of the respective brain areas. Furthermore, we took into account the spatial anatomical properties of the simulated brain structures and incor- porated a detailed numerical representation of 2·104 simulated neurons. We simulated the subthalamic nucleus (STN and the globus pallidus externus (GPe. Connections within the STN were governed by spike-timing dependent plasticity (STDP. In this way, we modeled the physiological and pathological activity of the considered brain structures. In particular, we investigated how plasticity could be exploited and how the model could be shifted from strongly synchronized (pathological activity to strongly desynchronized (healthy activity of the neuronal populations via CR stimulation of the STN neurons. Furthermore, we investigated the impact of specific stimulation parameters especially the electrode position on the stimulation outcome. Our model provides a step forward towards a biophysically realistic model of the brain areas relevant to the emergence of pathological neuronal activity in PD. Furthermore, our model constitutes a test bench for the optimization of both stimulation parameters and novel electrode geometries for efficient CR stimulation.

  2. MDS-UPDRS to assess non-motor symptoms after STN DBS for Parkinson's disease.

    Science.gov (United States)

    Jafari, Nickey; Pahwa, Rajesh; Nazzaro, Jules M; Arnold, Paul M; Lyons, Kelly E

    2016-01-01

    To determine if the non-motor sections of the Movement Disorder Society's (MDS) version of the Unified Parkinson's Disease Rating Scale (UPDRS) could supplement the original UPDRS as a patient completed assessment of changes in non-motor symptoms in Parkinson's disease (PD) patients after bilateral subthalamic nucleus (STN) deep brain stimulation (DBS). Thirty PD patients who underwent bilateral STN DBS were assessed using the total UPDRS and the non-motor sections of the MDS-UPDRS prior to surgery and one year following surgery. This study focuses on non-motor symptoms as assessed by Part I of the UPDRS and Part 1A and 1B of the MDS-UPDRS. One year following surgery, no individual non-motor symptoms or the total mentation score of the UPDRS were significantly changed. In comparison, the MDS-UPDRS showed significant improvements in sleep and urinary problems and a trend towards improvement in anxiety, constipation, daytime sleepiness, fatigue and pain. This study provides evidence that the MDS-UPDRS non-motor sections, when completed by the patients, can supplement the original version of the UPDRS as an effective method of measuring changes in non-motor symptoms after DBS. It also reinforces the benefits of bilateral STN DBS on non-motor symptoms of PD.

  3. Properties of BL Lac objects

    International Nuclear Information System (INIS)

    Wolfe, A.M.; Pittsburgh, University, Pittsburgh, Pa.)

    1980-01-01

    The properties of BL Lacertae objects are examined in light of their recently realized similarities to quasars and associations with galactic radiation. The criteria typically used to define BL Lac objects are analyzed, with attention given to radio spectra, optical continual, radio and optical variability, optical polarization and emission lines, and evidence that BL Lac objects and optically violent variables represent the most compact and strongly variable sources among the general class of quasars is discussed. Connections between BL Lac objects and the galaxies in which they have been observed to be embedded are discussed and it is pointed out that no low-luminosity quasars have been found to be associated with first-ranked giant ellipticals. Future observations which may clarify the properties and relationships of BL Lac objects are indicated

  4. What is dorso-lateral in the subthalamic Nucleus (STN)?--a topographic and anatomical consideration on the ambiguous description of today's primary target for deep brain stimulation (DBS) surgery.

    Science.gov (United States)

    Coenen, Volker A; Prescher, Andreas; Schmidt, Thorsten; Picozzi, Piero; Gielen, Frans L H

    2008-11-01

    The most frequently used target for DBS in advanced Parkinson Disease (PD) is the sensorimotor subthalamic nucleus (STN), anatomically referred to as dorso-lateral STN [3]. Ambiguities arise, regarding the true meaning of this description in the STN. Does "dorsal" indicate posterior or superior? At its best, this definition assigns two directions in space to a three-dimensional structure. This paper evaluates the ambiguity and describes the sensorimotor part of the STN in stereotactic space.

  5. Does early verbal fluency decline after STN implantation predict long-term cognitive outcome after STN-DBS in Parkinson's disease?

    Science.gov (United States)

    Borden, Alaina; Wallon, David; Lefaucheur, Romain; Derrey, Stéphane; Fetter, Damien; Verin, Marc; Maltête, David

    2014-11-15

    An early and transient verbal fluency (VF) decline and impairment in frontal executive function, suggesting a cognitive microlesion effect may influence the cognitive repercussions related to subthalamic nucleus deep brain stimulation (STN-DBS). Neuropsychological tests including semantic and phonemic verbal fluency were administered both before surgery (baseline), the third day after surgery (T3), at six months (T180), and at an endpoint multiple years after surgery (Tyears). Twenty-four patients (mean age, 63.5 ± 9.5 years; mean disease duration, 12 ± 5.8 years) were included. Both semantic and phonemic VF decreased significantly in the acute post-operative period (44.4 ± 28.2% and 34.3 ± 33.4%, respectively) and remained low at 6 months compared to pre-operative levels (decrease of 3.4 ± 47.8% and 10.8 ± 32.1%) (P < 0.05). Regression analysis showed phonemic VF to be an independent factor of decreased phonemic VF at six months. Age was the only independent predictive factor for incident Parkinson's disease dementia (PDD) (F (4,19)=3.4, P<0.03). An acute post-operative decline in phonemic VF can be predictive of a long-term phonemic VF deficit. The severity of this cognitive lesion effect does not predict the development of dementia which appears to be disease-related. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. Does unilateral basal ganglia activity functionally influence the contralateral side? What we can learn from STN stimulation in patients with Parkinson's disease.

    Science.gov (United States)

    Brun, Yohann; Karachi, Carine; Fernandez-Vidal, Sara; Jodoin, Nicolas; Grabli, David; Bardinet, Eric; Mallet, Luc; Agid, Yves; Yelnik, Jerome; Welter, Marie-Laure

    2012-09-01

    In humans, the control of voluntary movement, in which the corticobasal ganglia (BG) circuitry participates, is mainly lateralized. However, several studies have suggested that both the contralateral and ipsilateral BG systems are implicated during unilateral movement. Bilateral improvement of motor signs in patients with Parkinson's disease (PD) has been reported with unilateral lesion or high-frequency stimulation (HFS) of the internal part of the globus pallidus or the subthalamic nucleus (STN-HFS). To decipher the mechanisms of production of ipsilateral movements induced by the modulation of unilateral BG circuitry activity, we recorded left STN neuronal activity during right STN-HFS in PD patients operated for bilateral deep brain stimulation. Left STN single cells were recorded in the operating room during right STN-HFS while patients experienced, or did not experience, right stimulation-induced dyskinesias. Most of the left-side STN neurons (64%) associated with the presence of right dyskinesias were inhibited, with a significant decrease in burst and intraburst frequencies. In contrast, left STN neurons not associated with right dyskinesias were mainly activated (48%), with a predominant increase 4-5 ms after the stimulation pulse and a decrease in oscillatory activity. This suggests that unilateral neuronal STN modulation is associated with changes in the activity of the contralateral STN. The fact that one side of the BG system can influence the functioning of the other could explain the occurrence of bilateral dyskinesias and motor improvement observed in PD patients during unilateral STN-HFS, as a result of a bilateral disruption of the pathological activity in the corticosubcortical circuitry.

  7. A inter-relação das sialomucinas (antígenos Tn e Stn com o adenocarcinoma no esôfago de Barrett Relationship of the sialomucins (Tn and Stn antigens with adenocarcinoma in Barrett's esophagus

    Directory of Open Access Journals (Sweden)

    Plínio Conte de Faria Júnior

    2007-08-01

    Full Text Available OBJETIVO: O esôfago de Barrett (EB é conseqüência do refluxo gastroesofágico crônico e considerado fator de risco para o desenvolvimento de adenocarcinoma. Estudos do muco, em especial das mucinas ácidas representadas pelas sialomucinas presentes nas células caliciformes, mostraram que na metaplasia do tipo intestinal, o epitélio do órgão pode expressar antígenos denominados Tn e Stn. Estes antígenos já foram analisados em tumores gástricos e colônicos, porém não foram encontradas referências à sua utilização no EB. Este trabalho objetivou analisar estes antígenos em doentes com EB e em adenocarcinoma associado ao EB. MÉTODOS: Foram estudados, utilizando testes imunohistoquímicos, os antígenos Tn e Stn, nas biópsias endoscópicas de 29 doentes com EB, sete com adenocarcinoma no EB, além de oito indivíduos com epitélio esofágico normal. RESULTADOS: Nas células caliciformes, foi observada positividade para Stn em 100% dos casos e para Tn em 48% dos casos. Nas células colunares, o Stn foi sempre negativo, enquanto o Tn foi positivo em 100% dos casos. Entretanto, nos doentes com adenocarcinoma no EB, a positividade para ambos os antígenos foi de 100%. Nos indivíduos normais, houve positividade para o antígeno Tn e negatividade para Stn em todos os casos (100%. CONCLUSÃO: É provável que nos doentes com EB a positividade para o Tn, à semelhança do ocorrido quanto à positividade do mesmo antígeno nos portadores de adenocarcinoma, possa significar maior suscetibilidade para ocorrência futura de câncer. Assim, a pesquisa das sialomucinas poderá ser rotineiramente utilizada, contribuindo como fator prognóstico para desenvolvimento de adenocarcinoma no EB.OBJECIVE: Barrett's esophagus (BE is a consequence of chronic gastroesophageal reflux and is considered a risk factor for adenocarcinoma. The study of the mucus, especially acid mucins, such as the sialomucins in the goblet cells which characterize BE, showed

  8. blé et seigle

    African Journals Online (AJOL)

    SARAH

    hétérochromatine (séquences d'ADN non codante et riches en bases CG) des génomes d'un triticale primaire et leurs géniteurs, blé tendre et seigle d'une part, d'autre part de localiser les régions organisateurs nucléolaires (N.O.R) , les ...

  9. Den Blå Drage

    DEFF Research Database (Denmark)

    Ingerslev, Gitte Holten

    Denne publikation i serien Læselyst tjener tre formål. Den evaluerer læseprojektet Den blå drage i efteråret 2004 på Byskolen i Svendborg ? et projekt ledet af forfatteren Josefine Ottesen. Desuden gøres der rede for nogle af de teorier der støtter og begrunder arbejdsmåde og resultater i projektet...

  10. The human CTC1/STN1/TEN1 complex regulates telomere maintenance in ALT cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Chenhui; Jia, Pingping; Chastain, Megan; Shiva, Olga; Chai, Weihang, E-mail: wchai@wsu.edu

    2017-06-15

    Maintaining functional telomeres is important for long-term proliferation of cells. About 15% of cancer cells are telomerase-negative and activate the alternative-lengthening of telomeres (ALT) pathway to maintain their telomeres. Recent studies have shown that the human CTC1/STN1/TEN1 complex (CST) plays a multi-faceted role in telomere maintenance in telomerase-expressing cancer cells. However, the role of CST in telomere maintenance in ALT cells is unclear. Here, we report that human CST forms a functional complex localizing in the ALT-associated PML bodies (APBs) in ALT cells throughout the cell cycle. Suppression of CST induces telomere instabilities including telomere fragility and elevates telomeric DNA recombination, leading to telomere dysfunction. In addition, CST deficiency significantly diminishes the abundance of extrachromosomal circular telomere DNA known as C-circles and t-circles. Suppression of CST also results in multinucleation in ALT cells and impairs cell proliferation. Our findings imply that the CST complex plays an important role in regulating telomere maintenance in ALT cells. - Highlights: • CST localizes at telomeres and ALT-associated PML bodies in ALT cells throughout the cell cycle. • CST is important for promoting telomeric DNA replication in ALT cells. • CST deficiency decreases ECTR formation and increases T-SCE. • CST deficiency impairs ALT cell proliferation and results in multinucleation.

  11. Safety evaluation report related to the operation of Shearon Harris Nuclear Power Plant, Units 1 and 2. Docket Nos. STN 50-400 and STN 50-401

    International Nuclear Information System (INIS)

    1983-11-01

    The Safety Evaluation Report for the application filed by the Carolina Power and Light Company, as applicant and owner, for licenses to operate the Shearon Harris Nuclear Power Plant Units 1 and 2 (Docket Nos. 50-400 and 50-401) has been prepared by the Office of Nuclear Reactor Regulation of US Nuclear Regulatory Commission. The facility is located near Raleigh, North Carolina. Subject to favorable resolution of the items discussed in this report, the NRC staff concludes that the facility can be operated by the applicant without endangering the health and safety of the public

  12. Safety Evaluation Report related to the operation of Braidwood Station, Units 1 and 2 (Docket Nos. STN 50-456 and STN 50-457)

    International Nuclear Information System (INIS)

    1988-06-01

    In November 1983, the staff of the Nuclear Regulatory Commission issued its Safety Evaluation Report (NUREG-1002) regarding the application filed by the Commonwealth Edison Company, as applicant and owner, for a license to operate Braidwood Station, Units 1 and 2 (Docket Nos. 50-456 and 50-457). The first supplement to NUREG-1002 was issued in September 1986; the second supplement was issued in October 1986; the third supplement was issued in May 1987; the fourth supplement was issued in July 1987 in support of the full power license for Unit 1; the fifth supplement was issued in December 1987 in support of the low power license for Unit 2. This sixth supplement to NUREG-1002 is in support of the full-power license for Unit 2 and provides the status of items that remained unresolved at the time Supplement 5 was published. The facility is located in Reed Township, Will County, Illinois

  13. Safety Evaluation report related to the operation of Braidwood Station, Units 1 and 2 (Docket Nos. STN 50-456 and STN 50-457)

    International Nuclear Information System (INIS)

    1987-07-01

    In November 1983, the staff of the Nuclear Regulatory Commission issued its Safety Evaluation Report (NUREG-1002) regarding the application filed by the Commonwealth Edison Company, as applicant and owner, for a license to operate Braidwood Station, Units 1 and 2 (Docket Nos. 50-456 and 50-457). The first supplement to NUREG-1002 was issued in September 1986; the second supplement to NUREG-1002 was issued in October 1986; the third supplement to NUREG-1002 was issued in May 1987. This fourth supplement to NUREG-1002 reports the status of certain items that remained unresolved at the time Supplement 3 was published. The facility is located in Reed Township, Will County, Illinois

  14. Cognitive and Psychiatric Effects of STN versus GPi Deep Brain Stimulation in Parkinson's Disease: A Meta-Analysis of Randomized Controlled Trials.

    Directory of Open Access Journals (Sweden)

    Jia-Wei Wang

    Full Text Available Deep brain stimulation (DBS of either the subthalamic nucleus (STN or the globus pallidus interna (GPi can reduce motor symptoms in patients with Parkinson's disease (PD and improve their quality of life. However, the effects of STN DBS and GPi DBS on cognitive functions and their psychiatric effects remain controversial. The present meta-analysis was therefore performed to clarify these issues.We searched the PUBMED, EMBASE, and the Cochrane Central Register of Controlled Trials databases. Other sources, including internet-based clinical trial registries and grey literature sources, were also searched. After searching the literature, two investigators independently performed literature screens to assess the quality of the included trials and to extract the data. The outcomes included the effects of STN DBS and GPi DBS on multiple cognitive domains, depression, anxiety, and quality of life.Seven articles related to four randomized controlled trials that included 521 participants were incorporated into the present meta-analysis. Compared with GPi DBS, STN DBS was associated with declines in selected cognitive domains after surgery, including attention, working memory and processing speed, phonemic fluency, learning and memory, and global cognition. However, there were no significant differences in terms of quality of life or psychiatric effects, such as depression and anxiety, between the two groups.A selective decline in frontal-subcortical cognitive functions is observed after STN DBS in comparison with GPi DBS, which should not be ignored in the target selection for DBS treatment in PD patients. In addition, compared to GPi DBS, STN DBS does not affect depression, anxiety, and quality of life.

  15. Cognitive and Psychiatric Effects of STN versus GPi Deep Brain Stimulation in Parkinson's Disease: A Meta-Analysis of Randomized Controlled Trials.

    Science.gov (United States)

    Wang, Jia-Wei; Zhang, Yu-Qing; Zhang, Xiao-Hua; Wang, Yun-Peng; Li, Ji-Ping; Li, Yong-Jie

    2016-01-01

    Deep brain stimulation (DBS) of either the subthalamic nucleus (STN) or the globus pallidus interna (GPi) can reduce motor symptoms in patients with Parkinson's disease (PD) and improve their quality of life. However, the effects of STN DBS and GPi DBS on cognitive functions and their psychiatric effects remain controversial. The present meta-analysis was therefore performed to clarify these issues. We searched the PUBMED, EMBASE, and the Cochrane Central Register of Controlled Trials databases. Other sources, including internet-based clinical trial registries and grey literature sources, were also searched. After searching the literature, two investigators independently performed literature screens to assess the quality of the included trials and to extract the data. The outcomes included the effects of STN DBS and GPi DBS on multiple cognitive domains, depression, anxiety, and quality of life. Seven articles related to four randomized controlled trials that included 521 participants were incorporated into the present meta-analysis. Compared with GPi DBS, STN DBS was associated with declines in selected cognitive domains after surgery, including attention, working memory and processing speed, phonemic fluency, learning and memory, and global cognition. However, there were no significant differences in terms of quality of life or psychiatric effects, such as depression and anxiety, between the two groups. A selective decline in frontal-subcortical cognitive functions is observed after STN DBS in comparison with GPi DBS, which should not be ignored in the target selection for DBS treatment in PD patients. In addition, compared to GPi DBS, STN DBS does not affect depression, anxiety, and quality of life.

  16. Clustering environments of BL Lac objects

    Science.gov (United States)

    Wurtz, Ronald; Ellingson, Erica; Stocke, John T.; Yee, H. K. C.

    1993-01-01

    We report measurements of the amplitude of the BL Lac galaxy spatial covariance function, B(gb), for the fields of five BL Lacertae objects. We present evidence for rich clusters around MS 1207+39 and MS 1407+59, and confirm high richness for the cluster containing H0414+009. We discuss the ease of 3C 66 A and find evidence for a poor cluster based on an uncertain redshift of z = 0.444. These data suggest that at least some BL Lac objects are consistent with being FR 1 radio galaxies in rich clusters.

  17. BL-2a and BL-4a: certified uranium reference ores

    International Nuclear Information System (INIS)

    Steger, H.F.; Bowman, W.S.; Zechanowitsch, G.; Sutarno, R.

    1982-05-01

    Samples of two uranium ores BL-2a and BL-4a from Beaverlodge, Saskatchewan, were prepared as compositional reference materials to replace the similar certified ores, BL-2 and BL-4, of which the stock had been exhausted. Each ore was ground to minus 74 μm, blended in one lot and bottled in 200-g units. The homogeneity of the ores with respect to uranium was confirmed by both a neutron activation and a fluorimetric analytical procedure performed by two commercial laboratories. The recommended value for uranium is based on the results of one determination on each of 25 bottles by the volumetric-umpire method performed at CANMET. A statistical analysis of the data gave a recommended value for uranium of 0.426% for BL-2a and 0.1248% for BL-4a

  18. Application value of combined measurement of serum sTn, CA242, CA19-9 and CEA in the diagnosis of gastroenterological neoplasm

    International Nuclear Information System (INIS)

    Zhang Wanzhong; Chen Zhizhou; Fan Zhenfu

    2007-01-01

    To determine the application value of four serum tumor markers sTn, CA242, CA 19-9 and CEA in the diagnosis of gastroenterological neoplasm, the serum sTn, CA242, CA19-9 and CEA in 30 normal adult controls and 60 patients with gastroenterological neoplasm were measured by IRMA. The results showed that the serum sTn, CA242, CA19-9 and CEA levels in patients with gastric carcinoma or colorectal carcinoma were much higher than those in control group (P<0.01). The serum CEA, CA19-9 and CA242 levels in patients with colorectal carcinoma were significantly higher than those in patients with gastric carcinoma (P<0.01), but the serum sTn level in the former was markedly lower (P<0.01) than that in the latter. The sensitivity of tumor marker increased with the progress of clinical stages, with a considerably higher sensitivity for stage IV compared with stage I-II (P<0.01). The combined test of four tumor markers could be more sensitive than single test in detecting gastric carcinoma and colorectal carcinoma (P<0.05). Four tumor markers are useful for diagnosing gastroenterological neoplasm, and the combined measurement of 4 tumor markers could increase the sensitivity of detecting gastric carcinoma. (authors)

  19. Návrh IS pro Místní knihovnu města Jevišovice

    OpenAIRE

    Vašíček, Daniel

    2010-01-01

    Bakalářská práce pojednává o návrhu vytvoření databáze pro místní knihovnu města Jevišovice. Práce obsahuje vlastní návrh databáze a její vytvoření v programu Microsoft Access. Dále obsahuje vytvoření pracovního rozhraní pomocí VBA. The thesis deals with the concept of the Jevisovice city Local Library database creating. The thesis contains the concept of the database and its creation in the Microsoft Access software. Further, the work includes the creation of the VBA comunication interfac...

  20. Draft environmental statement related to the proposed manufacture of floating nuclear power plants (Docket No. STN 50-437)

    International Nuclear Information System (INIS)

    1976-10-01

    The proposed action is the issuance of a manufacturing license to Offshore Power Systems for the startup and operation of a proposed manufacturing facility located at Blount Island, Jacksonville, Florida (Docket No. STN 50-436). No nuclear fuel will be handled or stored at the manufacturing site. The plants will be fueled after they have been towed to and moored within breadwaters at specific offshore locations designated by the purchaser and after an operating license has been issued by the Nuclear Regulatory Commision. Each nuclear generating plant, mounted on a floating platform, has a net capacity of 1150 MWe. This energy is provided by a pressurized water reactor steam supply system consisting of a Westinghouse four-loop 3425 MWt unit with an ice-condenser containment system. When one or more of these units is located within a single breakwater, the installation is designated an offshore power station. 3 figs., 1 tab

  1. Comparison of imaging modalities and source-localization algorithms in locating the induced activity during deep brain stimulation of the STN.

    Science.gov (United States)

    Mideksa, K G; Singh, A; Hoogenboom, N; Hellriegel, H; Krause, H; Schnitzler, A; Deuschl, G; Raethjen, J; Schmidt, G; Muthuraman, M

    2016-08-01

    One of the most commonly used therapy to treat patients with Parkinson's disease (PD) is deep brain stimulation (DBS) of the subthalamic nucleus (STN). Identifying the most optimal target area for the placement of the DBS electrodes have become one of the intensive research area. In this study, the first aim is to investigate the capabilities of different source-analysis techniques in detecting deep sources located at the sub-cortical level and validating it using the a-priori information about the location of the source, that is, the STN. Secondly, we aim at an investigation of whether EEG or MEG is best suited in mapping the DBS-induced brain activity. To do this, simultaneous EEG and MEG measurement were used to record the DBS-induced electromagnetic potentials and fields. The boundary-element method (BEM) have been used to solve the forward problem. The position of the DBS electrodes was then estimated using the dipole (moving, rotating, and fixed MUSIC), and current-density-reconstruction (CDR) (minimum-norm and sLORETA) approaches. The source-localization results from the dipole approaches demonstrated that the fixed MUSIC algorithm best localizes deep focal sources, whereas the moving dipole detects not only the region of interest but also neighboring regions that are affected by stimulating the STN. The results from the CDR approaches validated the capability of sLORETA in detecting the STN compared to minimum-norm. Moreover, the source-localization results using the EEG modality outperformed that of the MEG by locating the DBS-induced activity in the STN.

  2. r-CBF brain SPET before surgery and during subthalamic nuclei (STN) high frequency stimulation (DBS) in Parkinson's Disease

    International Nuclear Information System (INIS)

    Gerundini, P.; Benti, R.; De Notaris, A.; Ferrari, M.; Raimondi, A.; Mariani, C.; Antonini, A.; Pezzoli, G.; Gaini, S.M.

    2002-01-01

    Deep brain stimulation (DBS) of the subthalamic nuclei (STN) can improve motor symptoms and reduces the need for medical therapy in severe Parkinson's Disease (PD). Moreover, DBS can affect, as the medical treatment, cerebral perfusion/metabolism even in cortical/subcortical areas not primarily involved in PD motor symptoms. Aim of the study was the assessment of r-CBF changes by mean of brain SPECT in severe PD .before surgery and during DBS of the STN. Methods. 14 PD patients (duration 15.2±5.1 ys; H and Y off-score 3.6±0.7) underwent STN electrode implantation. Residual motor dysfunction was assessed by UPDRS score up to one year after surgery. SPECT was performed after i.v. injection of Tc-99m ECD (740 MBq) in PD group before and 6-9 months after surgery and in 13 age matched normals. Standardized ROIs templates were applied in brain sections to generate perfusion ratios in the cerebral cortex and basal ganglia. Statistical Parametric Mapping (SPM) analysis of SPET studies was also obtained. Results: 6 months after surgery the mean UPDRS score improvement was 48.8±26.1% (DBS on) vs pre surgery. 8 patients (R+) had UPDRS improvement >50% (mean 67.7±8.8%); 6 patients (R-) had score improvement <50% (mean 22.9±18.9%). Before surgery, motor dysfunction during therapy was similar in R+ and R- groups (mean UPDRS 19.8±8.2 vs. 21.3±9.6). During BDS, UPDRS mean score without medical therapy was lower in R+ (15.6±5.6) vs. R- (35.0±12.4; p<0.001). ROIs and SPM analysis of pre-surgery SPET studies showed significant hypoperfusion (p<0.01) in the occipital gyri of PD vs control groups. No significant differences were found by comparing pre/post surgery SPECT patterns in whole PD group. However, in R- group SPECT showed significant hypoperfusion in pre-frontal areas, parietal and occipital gyri vs R+ patients (p<0.02) and controls (p<0.01). Before surgery, R+ group had borderline occipital hypoperfusion (p=0.04) and mild increase of putaminal perfusion (p=0.03) vs

  3. Freestanding rGO-SWNT-STN Composite Film as an Anode for Li Ion Batteries with High Energy and Power Densities

    Directory of Open Access Journals (Sweden)

    Taeseup Song

    2015-12-01

    Full Text Available Freestanding Si-Ti-Ni alloy particles/reduced graphene oxide/single wall carbon nanotube composites have been prepared as an anode for lithium ion batteries via a simple filtration method. This composite electrode showed a 9% increase in reversible capacity, a two-fold higher cycle retention at 50 cycles and a two-fold higher rate capability at 2 C compared to pristine Si-Ti-Ni (STN alloy electrodes. These improvements were attributed to the suppression of the pulverization of the STN active material by the excellent mechanical properties of the reduced graphene oxide-single wall carbon nanotube networks and the enhanced kinetics associated with both electron and Li ion transport.

  4. Stratifying Parkinson's Patients With STN-DBS Into High-Frequency or 60 Hz-Frequency Modulation Using a Computational Model.

    Science.gov (United States)

    Khojandi, Anahita; Shylo, Oleg; Mannini, Lucia; Kopell, Brian H; Ramdhani, Ritesh A

    2017-07-01

    High frequency stimulation (HFS) of the subthalamic nucleus (STN) is a well-established therapy for Parkinson's disease (PD), particularly the cardinal motor symptoms and levodopa induced motor complications. Recent studies have suggested the possible role of 60 Hz stimulation in STN-deep brain stimulation (DBS) for patients with gait disorder. The objective of this study was to develop a computational model, which stratifies patients a priori based on symptomatology into different frequency settings (i.e., high frequency or 60 Hz). We retrospectively analyzed preoperative MDS-Unified Parkinson's Disease Rating Scale III scores (32 indicators) collected from 20 PD patients implanted with STN-DBS at Mount Sinai Medical Center on either 60 Hz stimulation (ten patients) or HFS (130-185 Hz) (ten patients) for an average of 12 months. Predictive models using the Random Forest classification algorithm were built to associate patient/disease characteristics at surgery to the stimulation frequency. These models were evaluated objectively using leave-one-out cross-validation approach. The computational models produced, stratified patients into 60 Hz or HFS (130-185 Hz) with 95% accuracy. The best models relied on two or three predictors out of the 32 analyzed for classification. Across all predictors, gait and rest tremor of the right hand were consistently the most important. Computational models were developed using preoperative clinical indicators in PD patients treated with STN-DBS. These models were able to accurately stratify PD patients into 60 Hz stimulation or HFS (130-185 Hz) groups a priori, offering a unique potential to enhance the utilization of this therapy based on clinical subtypes. © 2017 International Neuromodulation Society.

  5. B-L mediated SUSY breaking with radiative B-L symmetry breaking

    International Nuclear Information System (INIS)

    Kikuchi, Tatsuru; Kubo, Takayuki

    2008-01-01

    We explore a mechanism of radiative B-L symmetry breaking in analogous to the radiative electroweak symmetry breaking. The breaking scale of B-L symmetry is related to the neutrino masses through the see-saw mechanism. Once we incorporate the U(1) B-L gauge symmetry in SUSY models, the U(1) B-L gaugino, Z-tilde B-L appears, and it can mediate the SUSY breaking (Z-prime mediated SUSY breaking) at around the scale of 10 6 GeV. Then we find a links between the neutrino mass (more precisly the see-saw or B-L scale of order 10 6 GeV) and the Z-prime mediated SUSY breaking scale. It is also very interesting that the gluino at the weak scale becomes relatively light, and almost compressed mass spectra for the gaugino sector can be realized in this scenario, which is very interesting in scope of the LHC.

  6. B-L violating supersymmetric couplings

    International Nuclear Information System (INIS)

    Ramond, P.

    1983-01-01

    We consider two problems: one is the possible effect of the breaking of Peccei-Quinn symmetry on the inflationary universe scenario; the other is the remark that even the minimal supersymmetric SU 5 theory contains B-L violating couplings which give rise to neutrino masses and family-diagonal proton decay. However the strength of these couplings is limited by the gauge hierarchy

  7. A Search for Low-Luminosity BL Lacertae Objects

    Science.gov (United States)

    Rector, Travis A.; Stocke, John T.; Perlman, Eric S.

    1999-05-01

    Many properties of BL Lacs have become explicable in terms of the ``relativistic beaming'' hypothesis, whereby BL Lacs are FR 1 radio galaxies viewed nearly along the jet axis. However, a possible problem with this model is that a transition population between beamed BL Lacs and unbeamed FR 1 galaxies has not been detected. A transition population of ``low-luminosity BL Lacs'' was predicted to exist in abundance in X-ray-selected samples such as the Einstein Extended Medium Sensitivity Survey (EMSS) by Browne & Marcha. However, these BL Lacs may have been misidentified as clusters of galaxies. We have conducted a search for such objects in the EMSS with the ROSAT High-Resolution Imager (HRI) here we present ROSAT HRI images, optical spectra, and VLA radio maps for a small number of BL Lacs that were previously misidentified in the EMSS catalog as clusters of galaxies. While these objects are slightly lower in luminosity than other EMSS BL Lacs, their properties are too similar to the other BL Lacs in the EMSS sample to ``bridge the gap'' between BL Lacs and FR 1 radio galaxies. Also, the number of new BL Lacs found is too low to alter significantly the X-ray luminosity function or value for the X-ray-selected EMSS BL Lac sample. Thus, these observations do not explain fully the discrepancy between the X-ray- and radio-selected BL Lac samples.

  8. VizieR Online Data Catalog: The CLASS BL Lac sample (Marcha+, 2013)

    Science.gov (United States)

    Marcha, M. J. M.; Caccianiga, A.

    2014-04-01

    This paper presents a new sample of BL Lac objects selected from a deep (30mJy) radio survey of flat spectrum radio sources (the CLASS blazar survey). The sample is one of the largest well-defined samples in the low-power regime with a total of 130 sources of which 55 satisfy the 'classical' optical BL Lac selection criteria, and the rest have indistinguishable radio properties. The primary goal of this study is to establish the radio luminosity function (RLF) on firm statistical ground at low radio luminosities where previous samples have not been able to investigate. The gain of taking a peek at lower powers is the possibility to search for the flattening of the luminosity function which is a feature predicted by the beaming model but which has remained elusive to observational confirmation. In this study, we extend for the first time the BL Lac RLF down to very low radio powers ~1022W/Hz, i.e. two orders of magnitude below the RLF currently available in the literature. In the process, we confirm the importance of adopting a broader, and more physically meaningful set of classification criteria to avoid the systematic missing of low-luminosity BL Lacs. Thanks to the good statistics we confirm the existence of weak but significant positive cosmological evolution for the BL Lac population, and we detect, for the first time the flattening of the RLF at L~1025W/Hz in agreement with the predictions of the beaming model. (1 data file).

  9. File sharing

    NARCIS (Netherlands)

    van Eijk, N.

    2011-01-01

    File sharing’ has become generally accepted on the Internet. Users share files for downloading music, films, games, software etc. In this note, we have a closer look at the definition of file sharing, the legal and policy-based context as well as enforcement issues. The economic and cultural

  10. The gBL transport equations

    International Nuclear Information System (INIS)

    Mynick, H.E.

    1989-05-01

    The transport equations arising from the ''generalized Balescu- Lenard'' (gBL) collision operator are obtained, and some of their properties examined. The equations contain neoclassical and turbulent transport as two special cases, having the same structure. The resultant theory offers potential explanation for a number of results not well understood, including the anomalous pinch, observed ratios of Q/ΓT on TFTR, and numerical reproduction of ASDEX profiles by a model for turbulent transport invoked without derivation, but by analogy to neoclassical theory. The general equations are specialized to consideration of a number of particular transport mechanisms of interest. 10 refs

  11. Motor and non-motor circuitry activation induced by subthalamic nucleus deep brain stimulation (STN DBS) in Parkinson’s disease patients: Intraoperative fMRI for DBS

    Science.gov (United States)

    Knight, Emily J.; Testini, Paola; Min, Hoon-Ki; Gibson, William S.; Gorny, Krzysztof R.; Favazza, Christopher P.; Felmlee, Joel P.; Kim, Inyong; Welker, Kirk M.; Clayton, Daniel A.; Klassen, Bryan T.; Chang, Su-youne; Lee, Kendall H.

    2015-01-01

    Objective To test the hypothesis suggested by previous studies that subthalamic nucleus (STN) deep brain stimulation (DBS) in patients with PD would affect the activity of both motor and non-motor networks, we applied intraoperative fMRI to patients receiving DBS. Patients and Methods Ten patients receiving STN DBS for PD underwent intraoperative 1.5T fMRI during high frequency stimulation delivered via an external pulse generator. The study was conducted between the dates of January 1, 2013 and September 30, 2014. Results We observed blood oxygen level dependent (BOLD) signal changes (FDR<.001) in the motor circuitry, including primary motor, premotor, and supplementary motor cortices, thalamus, pedunculopontine nucleus (PPN), and cerebellum, as well as in the limbic circuitry, including cingulate and insular cortices. Activation of the motor network was observed also after applying a Bonferroni correction (p<.001) to our dataset, suggesting that, across subjects, BOLD changes in the motor circuitry are more consistent compared to those occurring in the non-motor network. Conclusions These findings support the modulatory role of STN DBS on the activity of motor and non-motor networks, and suggest complex mechanisms at the basis of the efficacy of this treatment modality. Furthermore, these results suggest that, across subjects, BOLD changes in the motor circuitry are more consistent compared to those occurring in the non-motor network. With further studies combining the use of real time intraoperative fMRI with clinical outcomes in patients treated with DBS, functional imaging techniques have the potential not only to elucidate the mechanisms of DBS functioning, but also to guide and assist in the surgical treatment of patients affected by movement and neuropsychiatric disorders. PMID:26046412

  12. Bump masses for BL Her stars

    International Nuclear Information System (INIS)

    Davis, C.G.

    1982-01-01

    The masses of classical Cepheids can be determined by using the phase of the Hertzsprung bump on the light or velocity curve, Cox-Stewart opacities, and nonlinear pulsation theory. The fact that these bump masses are some 60% lower than the evolutionary masses raises some questions about this approach. In support of our method, we calculate the light curve for BL Her, a population II Cepheid, with an observed bump on the declining portion of its light curve. The nonlinear hydrodynamic model we use (Davis and Davison - 1978) resolves the light curve by dynamic zoning and allows us the opportunity to make a direct comparison of the calculated light curve to the observations, using a prescribed mass, luminosity and effective temperature. The parameters for BL Her are from a linear model (Hodson, Cox, and King - 1982) that has nearly the correct period (1./sup d/2) and the correct period ratio from resonance theory (π 2 /π 0 = 0.53) for a bump to appear on the declining portion of the light curve as observed. These parameters are: M = 0.55 M, L = 95.0 L, and T/sub eff/ = 6500 K. This mass is near the evolutionary mass as described by Schwartzschild and Haerm (1970). The model results agree well with the observations and the color-T/sub eff/ relation has the same slope as that observed for RR Lyrae stars by the Oke, Giver and Searle (1965) relationship

  13. Masses and pulsations of BL Herculis variables

    International Nuclear Information System (INIS)

    Hodson, S.W.; Cox, A.N.; King, D.S.

    1981-01-01

    From linear results, the masses of BL Her variables must be nearer to 0.55 M /sub sun/ than 0.75 M /sub sun/ if the bump phase transition (resonance) is to be located anywhere near the observed period range of 1./sup d/5 to 1./sup d/7. The nonlinear results are consistent with the Simon resonance concept, but demonstrate that light and velocity curve shapes are a nonlinear phenomenon that require nonlinear period ratios to display the resonances only in the narrow, observed range of 1./sup d/5 to 1./sup d/7. The mass near 0.55 M /sub sun/ is in good agreement with evolution calculations (Sweigart and Gross, 1976) and nonlinear pulsation studies of Carson, Stothers, and Vemury (1981) and Stothers

  14. No evidence for radio-quiet BL Lacertae objects

    International Nuclear Information System (INIS)

    Stocke, J.T.; Morris, S.L.; Gioia, I.; Maccacaro, T.; Schild, R.E.

    1990-01-01

    Using a large, flux-limited sample of faint X-ray sources, a search has been conducted for radio-quiet BL Lacertae objects. None has been found. Thirty-two X-ray-selected BL Lac objects and BL Lac candidates have been found within the sources of the Einstein Medium Sensitivity Survey (EMSS). Thirty-one of these have been observed with the VLA and all have been detected at 5 GHz. While the optical magnitudes of the EMSS BL Lac objects range from 17 to 20.8, their radio-to-optical spectral indices occupy a very small range. The very bright X-ray-selected BL Lac objects like PKS 2155-304 and Markarian 501 have similar range values. Therefore, unlike the clear dichotomy between radio-loud quasars and radio-quiet QSOs, there is no evidence for two populations of Lacertids distinguished by radio loudness. 43 refs

  15. 76 FR 21946 - Proposed Collection; Comment Request for Form 990-BL; Schedule A (Form 990-BL), Form 6069

    Science.gov (United States)

    2011-04-19

    ... Initial Excise Tax Return for Black Lung Benefit Trusts and Certain Related Persons, and Form 6069, Return of Excise Tax on Excess Contributions to Black Lung Benefit Trust Under Section 4953 and Computation...: Form 990-BL, Schedule A (Form 990-BL), Information and Initial Excise Tax Return for Black Lung Benefit...

  16. ACONC Files

    Data.gov (United States)

    U.S. Environmental Protection Agency — ACONC files containing simulated ozone and PM2.5 fields that were used to create the model difference plots shown in the journal article. This dataset is associated...

  17. XML Files

    Science.gov (United States)

    ... this page: https://medlineplus.gov/xml.html MedlinePlus XML Files To use the sharing features on this page, please enable JavaScript. MedlinePlus produces XML data sets that you are welcome to download ...

  18. 831 Files

    Data.gov (United States)

    Social Security Administration — SSA-831 file is a collection of initial and reconsideration adjudicative level DDS disability determinations. (A few hearing level cases are also present, but the...

  19. On the close environment of BL Lacertae objects

    Energy Technology Data Exchange (ETDEWEB)

    Falomo, R. (Osservatorio Astronomico di Padova (Italy)); Melnick, J. (European Southern Observatory, Santiago (Chile)); Tanzi, E.G. (Consiglio Nazionale delle Ricerche, Milan (Italy))

    1990-06-21

    The local environment of BL Lacertae objects, which resemble quasars but lack emission lines, is poorly understood. In the few cases where the surrounding nebulosity has been studied in detail, it is consistent with the presence of a giant elliptical galaxy, but the evidence that the BL Lac and the putative galaxy are physically associated rests solely on their positional coincidence. An alternative hypothesis, that BL Lacs are gravitationally lensed and that the surrounding emission is from the foreground lensing object, gains some support from a number of observations which reveal less than perfect alignment between BL Lacs and surrounding emission. We have begun a systematic programme of high-resolution imaging aimed at understanding in a general way the local environment of BL Lacs. Here we describe a first series of images, which show the presence of emission features around most of the BL Lacs observed. Typically, this emission is close (<5 arcsec) to the BL Lac, and faint (m{sub R} = 21). We discuss the interpretation of these companions in terms of both interacting objects and gravitational lenses. (author).

  20. Behavioral profiles of three C57BL/6 substrains

    Directory of Open Access Journals (Sweden)

    Naoki Matsuo

    2010-06-01

    Full Text Available C57BL/6 inbred strains of mice are widely used in knockout and transgenic research. To evaluate the loss-of-function and gain-of-function effects of the gene of interest, animal behaviors are often examined. However, an issue of C57BL/6 substrains that is not always appreciated is that behaviors are known to be strongly influenced by genetic background. To investigate the behavioral characteristics of C57BL/6 substrains, we subjected C57BL/6J, C57BL/6N, and C57BL/6C mice to a behavior test battery. We performed both a regular-scale analysis, in which experimental conditions were tightly-controlled, and meta-analysis from large number of behavioral data that we have collected so far through the comprehensive behavioral test battery applied to 700-2,200 mice in total. Significant differences among the substrains were found in the results of various behavioral tests, including the open field, rotarod, elevated plus maze, prepulse inhibition, Porsolt forced swim, and spatial working memory version of the 8-arm radial maze. Our results show a divergence of behavioral performance in C57BL/6 substrains, which suggest that small genetic differences may have a great influence on behavioral phenotypes. Thus, the genetic background of different substrains should be carefully chosen, equated, and considered in the interpretation of mutant behavioral phenotypes.

  1. The Discovery of Low-Luminosity BL Lacs

    Science.gov (United States)

    Rector, Travis A.; Stocke, John T.

    1995-12-01

    Many of the properties of BL Lacs have become explicable in terms of the ``relativistic beaming'' hypothesis whereby BL Lacs are ``highly beamed'' FR-I radio galaxies (i.e. our line of sight to these objects is nearly along the jet axis). Further, radio-selected BL Lacs (RBLs) are believed to be seen nearly ``on-axis'' (the line-of-sight angle theta ~ 8deg ) while X-ray selected BL Lacs (XBLs) are seen at larger angles (theta ~ 30deg ; the X-ray emitting jet is believed to be less collimated). However, a major problem with this model was that a transition population between beamed BL Lacs and unbeamed FR-Is had not been detected. Low-luminosity BL Lacs may be such a transition population, and were predicted to exist by Browne and Marcha (1993). We present ROSAT HRI images, VLA radio maps and optical spectra which confirm the existence of low-luminosity BL Lacs, objects which were previously mis-identified in the EMSS catalog as clusters of galaxies. Thus our results strengthen the relativistic beaming hypothesis.

  2. Studies on a transplantable C57BL/6 mouse lymphoma

    International Nuclear Information System (INIS)

    Kendall, C.E.

    1977-01-01

    A C57BL/6 mouse lymphoma was demonstrated to be of T cell origin by treating the lymphoma cells with anti-Thy 1.2 antisera in a complement-dependent cytotoxicity test. The lymphoma's growth pattern was described using flow microfluorometric determinations and spleen weight progression. C-type particles were identified in electron micrographs of the lymphoma. C57BL/6 mice were immunized against the lymphoma by injecting x-ray inactivated lymphoma cells into the mice. Protection of immunized mice against live lymphoma cells demonstrated tumor antigens on the lymphoma cells. The success of immunization was found to depend on: route of injection, antigen dosage, state of the antigen, number of injections and the vaccination-challenge interval. Attempts were made to passively transfer immunity from immunized C57BL/6 mice which had survived lymphoma challenge to non-treated, syngeneic mice. The route of immunization in the donors influenced the success of passively transferred immunity in the recipients. Serum from days 1 to 3 and days 11 to death (day 17) had an enhancing effect on lymphoma growth. However, sera from days 5 to 9 retarded lymphoma growth. The C57BL/6 lymphoma cells were injected into rabbits and other strains of mice to demonstrate tumor specificity. The lymphoma did not grow in rabbits and only grew in one mouse strain. This strain had the same major histocompatibility loci as C57BL/6 mice. Crosses were made between C57BL/6 mice and a resistant strain of mice (DBA/2 mice). The F 1 hybrids were found to be less susceptible to the lymphoma than the C57BL/6 strain. Sublethal x-irradiation of the F 1 mice decreased its ability to resist the C57BL/6 lymphoma. Immunization with x-ray inactivated C57BL/6 lymphoma cells increased survival after challenge with lymphoma in the F 1 mice

  3. The cosmic evolution of Fermi BL Lacertae objects

    International Nuclear Information System (INIS)

    Ajello, M.; Romani, R. W.; Shaw, M. S.; Healey, S. E.; Michelson, P. F.; Gasparrini, D.; Bolmer, J.; Cotter, G.; Potter, W. J.; Finke, J.; Greiner, J.; Rau, A.; Schady, P.; King, O.; Max-Moerbeck, W.; Readhead, A. C. S.; Richards, J. L.

    2014-01-01

    Fermi has provided the largest sample of γ-ray-selected blazars to date. In this work we use a uniformly selected set of 211 BL Lacertae (BL Lac) objects detected by Fermi during its first year of operation. We obtained redshift constraints for 206 out of the 211 BL Lac objects in our sample, making it the largest and most complete sample of BL Lac objects available in the literature. We use this sample to determine the luminosity function of BL Lac objects and its evolution with cosmic time. We find that for most BL Lac classes the evolution is positive, with a space density peaking at modest redshift (z ≈ 1.2). Low-luminosity, high-synchrotron-peaked (HSP) BL Lac objects are an exception, showing strong negative evolution, with number density increasing for z ≲ 0.5. Since this rise corresponds to a drop-off in the density of flat-spectrum radio quasars (FSRQs), a possible interpretation is that these HSPs represent an accretion-starved end state of an earlier merger-driven gas-rich phase. We additionally find that the known BL Lac correlation between luminosity and photon spectral index persists after correction for the substantial observational selection effects with implications for the so-called 'blazar sequence'. Finally, by estimating the beaming corrections to the luminosity function, we find that BL Lac objects have an average Lorentz factor of γ=6.1 −0.8 +1.1 , and that most are seen within 10° of the jet axis.

  4. Optical Spectra Evolution of BL Lac Objects XW Bi1,∗ , BZ Wang2

    Indian Academy of Sciences (India)

    low-frequency peaked BL Lac objects (LBLs) is steeper than the high- frequency peaked BL Lac ... vision of BL Lac objects. Many investigators .... We are grateful to the Scientific Research Foundation of the Education Department of Yunnan ...

  5. Infrared polarimetry and photometry of BL Lac objects. 3

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, P A; Brand, P W.J.L. [Edinburgh Univ. (UK). Dept. of Astronomy; Impey, C D [Hawaii Univ., Honolulu (USA). Inst. for Astronomy; Williams, P M [UKIRT, Hilo, HI (USA)

    1984-10-15

    The data presented here is part of a continuing monitoring programme of BL lac objects with J, H and K photometry and polarimetry. A total of 30 BL Lac objects have now been observed photometrically. Infrared polarimetry has also been obtained for 24 of these objects. The sample is sufficiently large to examine statistically, and several important correlations have emerged. Internight variations and wavelength dependence of polarization indicate that BL Lac objects, as a class, may be understood in terms of a relatively simple two-component model.

  6. HYDROGEOLOGIC CHARACTERIZATION OF THE U-3bl COLLAPSE ZONE

    International Nuclear Information System (INIS)

    Bechtel Nevada and National Security Technologies, LLC

    2006-01-01

    The U-3bl collapse crater was formed by an underground nuclear test in August 1962. This crater and the adjoining U-3ax crater were subsequently developed and used as a bulk low-level radioactive waste disposal cell (U-3ax/bl), which is part of the Area 3 Radioactive Waste Management Site at the Nevada Test Site (NTS). Various investigations have been conducted to assess the hydrogeologic characteristics and properties in the vicinity of the U-3ax/bl waste disposal cell. This report presents data from one of these investigations, conducted in 1996. Also included in this report is a review of pertinent nuclear testing records, which shows that the testing operations and hydrogeologic setting of the U-3ax/bl site were typical for the period and location of testing

  7. The near-infrared continua of BL Lacertae objects

    International Nuclear Information System (INIS)

    Allen, D.A.; Ward, M.J.; Hyland, A.R.

    1982-01-01

    Accurate photometry at J, H and K (1.2 to 2.2 μm) has been secured of 53 BL Lac objects. A power-law continuum is an exceptionally good fit to the data, and the colours are distinct from those of quasars. There is no indication of the additional infrared continuum seen in quasars and which is believed to arise in circumnuclear dust. We argue that dust is very scarce in the nuclei of BL Lac objects, and thus we expect gas to be equally scarce. Hence we attribute the lack of optical emission lines to an absence of ionized nuclear gas. We further argue that BL Lac objects could underlie quasars, the latter exhibiting line and thermal continuum emission at ultraviolet, optical and near-infrared wavelengths due to the presence of circumnuclear gas and dust. The strong-lined optically violent variable quasars have colours typical of BL Lac objects rather than quasars, and may represent intermediate cases. The JHK colours of the BL Lac objects overlap with those of the empty-radio-field infrared sources. Such objects probably represent the redder extreme of a range of spectral indices in BL Lac objects. (author)

  8. Radium-226 in certified uranium reference ores DL-1a, BL-4a, DH-1a and BL-5

    International Nuclear Information System (INIS)

    Smith, C.W.; Steger, H.F.

    1983-05-01

    Radium-226 radioactivity in uranium reference ores BL-4a and BL-5 and uranium-thorium reference ores DL-1a and DH-1a was determined in an interlaboratory program. Twelve of thirteen participants used certified radium solutions from the United States National Bureau of Standards (NBS) for calibration purposes. Recommended values of sup(226)Ra activity and associated parameters were calculated by statistical treatment of the results. In all cases, the recommended values are within 2 percent of activities predicted assuming secular equilibrium in the sup(238)U decay series. The recommended values for radium activity are 1.40, 15.5, 31.5 and 857 Bq/ for DL-1a, BL-4a, DH-1a and BL-5, respectively

  9. JENDL special purpose file

    International Nuclear Information System (INIS)

    Nakagawa, Tsuneo

    1995-01-01

    In JENDL-3,2, the data on all the reactions having significant cross section over the neutron energy from 0.01 meV to 20 MeV are given for 340 nuclides. The object range of application extends widely, such as the neutron engineering, shield and others of fast reactors, thermal neutron reactors and nuclear fusion reactors. This is a general purpose data file. On the contrary to this, the file in which only the data required for a specific application field are collected is called special purpose file. The file for dosimetry is a typical special purpose file. The Nuclear Data Center, Japan Atomic Energy Research Institute, is making ten kinds of JENDL special purpose files. The files, of which the working groups of Sigma Committee are in charge, are listed. As to the format of the files, ENDF format is used similarly to JENDL-3,2. Dosimetry file, activation cross section file, (α, n) reaction data file, fusion file, actinoid file, high energy data file, photonuclear data file, PKA/KERMA file, gas production cross section file and decay data file are described on their contents, the course of development and their verification. Dosimetry file and gas production cross section file have been completed already. As for the others, the expected time of completion is shown. When these files are completed, they are opened to the public. (K.I.)

  10. Infrared polarimetry and photometry of BL Lac objects

    Energy Technology Data Exchange (ETDEWEB)

    Impey, C D; Brand, P W.J.L. [Edinburgh Univ. (UK); Wolstencroft, R D; Williams, P M [Royal Observatory, Edinburgh (UK)

    1982-07-01

    Infrared polarimetry and photometry have been obtained for a sample of 18 BL Lac objects. The data covers a period of one year and is part of a continuing monitoring programme; all observations were in the J,H and K wavebands. Large and variable degrees of polarization are a common property of the sample. Two BL Lac objects show wavelength-dependent polarization, with the polarization increasing towards shorter wavelengths, and two objects show evidence for position angle rotations over a five-day period. The relationship between changes in polarized and total flux is also discussed. The BL Lac objects cover an enormous range of infrared luminosity; the three most luminous having Lsub(IR) > 10/sup 46/ erg s/sup -1/ and the other end of the range having infrared luminosities similar to normal elliptical galaxies. These are the first published infrared polarimetric observations for eight of the sample.

  11. Time-dependent inhomogeneous jet models for BL Lac objects

    Science.gov (United States)

    Marlowe, A. T.; Urry, C. M.; George, I. M.

    1992-05-01

    Relativistic beaming can explain many of the observed properties of BL Lac objects (e.g., rapid variability, high polarization, etc.). In particular, the broadband radio through X-ray spectra are well modeled by synchrotron-self Compton emission from an inhomogeneous relativistic jet. We have done a uniform analysis on several BL Lac objects using a simple but plausible inhomogeneous jet model. For all objects, we found that the assumed power-law distribution of the magnetic field and the electron density can be adjusted to match the observed BL Lac spectrum. While such models are typically unconstrained, consideration of spectral variability strongly restricts the allowed parameters, although to date the sampling has generally been too sparse to constrain the current models effectively. We investigate the time evolution of the inhomogeneous jet model for a simple perturbation propagating along the jet. The implications of this time evolution model and its relevance to observed data are discussed.

  12. The B-L scotogenic models for Dirac neutrino masses

    Science.gov (United States)

    Wang, Weijian; Wang, Ruihong; Han, Zhi-Long; Han, Jin-Zhong

    2017-12-01

    We construct the one-loop and two-loop scotogenic models for Dirac neutrino mass generation in the context of U(1)_{B-L} extensions of standard model. It is indicated that the total number of intermediate fermion singlets is uniquely fixed by the anomaly free condition and the new particles may have exotic B-L charges so that the direct SM Yukawa mass term \\bar{ν }_Lν _R\\overline{φ ^0} and the Majorana mass term (m_N/2)\\overline{ν _R^C}ν _R are naturally forbidden. After the spontaneous breaking of the U(1)_{B-L} symmetry, the discrete Z2 or Z3 symmetry appears as the residual symmetry and gives rise to the stability of intermediate fields as DM candidates. Phenomenological aspects of lepton flavor violation, DM, leptogenesis and LHC signatures are discussed.

  13. Von Blüten, Göttern und Gelehrten

    OpenAIRE

    Zotter, Astrid

    2013-01-01

    Der Sanskrittext Puṣpacintāmaṇi (PuCi) behandelt Blüten als Darbringungen (upacāra) im wichtigsten hinduistischen Verehrungsritual, der pūjā. In ca. 400 Strophen werden über 200 Namen von Blüten genannt und für verschiedene Gottheiten und pūjās als geeignete oder ungeeignete Gaben vorgeschrieben. Der Text ist eine Kompilation (Nibandha), in der die Inhalte aus 47 namentlich genannten Quellentexten referiert werden. Ziel der Dissertation ist es nicht nur, diesen Text, der 1966 zum ersten Ma...

  14. Optical polarimetry of quasi-stellar and BL Lac objects

    International Nuclear Information System (INIS)

    Wills, D.; Wills, B.J.; Breger, M.; Hsu, J.

    1980-01-01

    We report observations of 39 extragalactic objects, using a new polarimeter at McDonald Observatory. Most of the observations were made in January and June 1980. Some new BL Lac objects were found, and some of the previously known members of this class were also observed. Polarization exceeding 3--4 times the formal rms uncertainties was found in about ten QSO's. Rapid changes of polarization and/or position angle occurred in some of the BL Lacertae objects, usually with <0.1-mag change in the total light

  15. Highlights of SPring-8 BL23SU in 2001

    CERN Document Server

    Agui, A; Nakatani, T; Yokoya, A; Yoshigoe, A

    2002-01-01

    BL23SU in SPring-8 is a soft x-ray beamline for the material science project in JAERI. The insertion device, monochromator and other beamline equipments have been installed or developed. The beamline maintenance has also been continued. We report highlights of these activities for the BL23SU in 2001. Specially, it has proceeded with the countermeasure against the rise in the maximum radiant power, toward the low-energy operation realization. And, improvement in that precision proceeds through the COD correlation to the ID drive as well.

  16. Safety evaluation report related to the preliminary design of the Standard Nuclear Steam Supply Reference System, RESAR SP/90 (Docket No. STN 50-601)

    International Nuclear Information System (INIS)

    1991-04-01

    On October 24, 1983, the Westinghouse Electric Corporation tendered its application for a preliminary design approval of the advanced pressurized-water reactor design for the SP/90 reactor. The Westinghouse Reference Safety Analysis Report (RESAR SP/90, Docket No. STN 50-601), describing the design of the facility, was submitted from October 24, 1983 through March 9, 1987. Staff of the US Nuclear Regulatory Commission, Office of Nuclear Reactor Regulation, has prepared this safety evaluation report of the RESAR SP/90 on the basis of its review. Because of the stage of the design, there are open issues that have not been resolved. These issues are discussed in detail throughout this report, and a summary is provided in Section 1.6 of this report. The applicant will be required to address these and any additional such concerns that may be raised during the course of the staff's review of advanced light-water reactors in support of a final design approval application. This report shall not constitute a commitment to issue a permit or license or in any way affect the authority of the Commission, its adjudicatory boards, and other presiding officers in any proceeding under Subpart G of Title 10 of the Code of Federal Regulations, Part 2

  17. HUD GIS Boundary Files

    Data.gov (United States)

    Department of Housing and Urban Development — The HUD GIS Boundary Files are intended to supplement boundary files available from the U.S. Census Bureau. The files are for community planners interested in...

  18. The gravitational wave spectrum from cosmological B-L breaking

    International Nuclear Information System (INIS)

    Buchmueller, W.; Domcke, V.; Kamada, K.; Schmitz, K.

    2013-05-01

    Cosmological B-L breaking is a natural and testable mechanism to generate the initial conditions of the hot early universe. If B-L is broken at the grand unification scale, the false vacuum phase drives hybrid inflation, ending in tachyonic preheating. The decays of heavy B-L Higgs bosons and heavy neutrinos generate entropy, baryon asymmetry and dark matter and also control the reheating temperature. The different phases in the transition from inflation to the radiation dominated phase produce a characteristic spectrum of gravitational waves. We calculate the complete gravitational wave spectrum due to inflation, preheating and cosmic strings, which turns out to have several features. The production of gravitational waves from cosmic strings has large uncertainties, with lower and upper bounds provided by Abelian Higgs strings and Nambu-Goto strings, implying Ω GW h 2 ∝10 -13 -10 -8 , much larger than the spectral amplitude predicted by inflation. Forthcoming gravitational wave detectors such as eLISA, advanced LIGO and BBO/DECIGO will reach the sensitivity needed to test the predictions from cosmological B-L breaking.

  19. The Optical Microvariability and Spectral Changes of the BL ...

    Indian Academy of Sciences (India)

    strong bluer-when-brighter chromatism was found while different spectral behaviours were found on intranight time scales. Key words. BL lacertae objects: individual (S5 0716+714)—galaxies: active—galaxies: photometry. 1. Introduction. Since the development of CCD in the 80s, microvariability of blazars has been.

  20. RADIO-WEAK BL LAC OBJECTS IN THE FERMI ERA

    Energy Technology Data Exchange (ETDEWEB)

    Massaro, F.; Marchesini, E. J. [Dipartimento di Fisica, Università degli Studi di Torino (UniTO), via Pietro Giuria 1, I-10125 Torino (Italy); D’Abrusco, R.; Smith, Howard A. [Smithsonian Astrophysical Observatory, 60 Garden Street, 02138 Cambridge, MA (United States); Masetti, N. [INAF—Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna, via Gobetti 101, I-40129, Bologna (Italy); Andruchow, I. [Facultad de Ciencias Astronómicas y Geofísicas, Universidad Nacional de La Plata, Paseo del Bosque, B1900FWA, La Plata (Argentina)

    2017-01-10

    The existence of “radio-weak BL Lac objects” (RWBLs) has been an open question, and has remained unsolved since the discovery that quasars could be radio-quiet or radio-loud. Recently, several groups identified RWBL candidates, mostly found while searching for low-energy counterparts of the unidentified or unassociated gamma-ray sources listed in the Fermi catalogs. Confirming RWBLs is a challenging task since they could be confused with white dwarfs (WDs) or weak emission line quasars (WELQs) when there are not sufficient data to precisely draw their broadband spectral energy distribution, and their classification is mainly based on a featureless optical spectra. Motivated by the recent discovery that Fermi BL Lacs appear to have very peculiar mid-IR emission, we show that it is possible to distinguish between WDs, WELQs, and BL Lacs using the [3.4]–[4.6]–[12] μ m color–color plot built using the WISE magnitudes when the optical spectrum is available. On the basis of this analysis, we identify WISE J064459.38+603131 and WISE J141046.00+740511.2 as the first two genuine RWBLs, both potentially associated with Fermi sources. Finally, to strengthen our identification of these objects as true RWBLs, we present multifrequency observations for these two candidates to show that their spectral behavior is indeed consistent with that of the BL Lac population.

  1. RADIO-WEAK BL LAC OBJECTS IN THE FERMI ERA

    International Nuclear Information System (INIS)

    Massaro, F.; Marchesini, E. J.; D’Abrusco, R.; Smith, Howard A.; Masetti, N.; Andruchow, I.

    2017-01-01

    The existence of “radio-weak BL Lac objects” (RWBLs) has been an open question, and has remained unsolved since the discovery that quasars could be radio-quiet or radio-loud. Recently, several groups identified RWBL candidates, mostly found while searching for low-energy counterparts of the unidentified or unassociated gamma-ray sources listed in the Fermi catalogs. Confirming RWBLs is a challenging task since they could be confused with white dwarfs (WDs) or weak emission line quasars (WELQs) when there are not sufficient data to precisely draw their broadband spectral energy distribution, and their classification is mainly based on a featureless optical spectra. Motivated by the recent discovery that Fermi BL Lacs appear to have very peculiar mid-IR emission, we show that it is possible to distinguish between WDs, WELQs, and BL Lacs using the [3.4]–[4.6]–[12] μ m color–color plot built using the WISE magnitudes when the optical spectrum is available. On the basis of this analysis, we identify WISE J064459.38+603131 and WISE J141046.00+740511.2 as the first two genuine RWBLs, both potentially associated with Fermi sources. Finally, to strengthen our identification of these objects as true RWBLs, we present multifrequency observations for these two candidates to show that their spectral behavior is indeed consistent with that of the BL Lac population.

  2. The gravitational wave spectrum from cosmological B-L breaking

    Energy Technology Data Exchange (ETDEWEB)

    Buchmueller, W.; Domcke, V.; Kamada, K. [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Schmitz, K. [Tokyo Univ., Kashiwa (Japan). Kavli IPMU (WPI)

    2013-05-15

    Cosmological B-L breaking is a natural and testable mechanism to generate the initial conditions of the hot early universe. If B-L is broken at the grand unification scale, the false vacuum phase drives hybrid inflation, ending in tachyonic preheating. The decays of heavy B-L Higgs bosons and heavy neutrinos generate entropy, baryon asymmetry and dark matter and also control the reheating temperature. The different phases in the transition from inflation to the radiation dominated phase produce a characteristic spectrum of gravitational waves. We calculate the complete gravitational wave spectrum due to inflation, preheating and cosmic strings, which turns out to have several features. The production of gravitational waves from cosmic strings has large uncertainties, with lower and upper bounds provided by Abelian Higgs strings and Nambu-Goto strings, implying {Omega}{sub GW}h{sup 2}{proportional_to}10{sup -13}-10{sup -8}, much larger than the spectral amplitude predicted by inflation. Forthcoming gravitational wave detectors such as eLISA, advanced LIGO and BBO/DECIGO will reach the sensitivity needed to test the predictions from cosmological B-L breaking.

  3. Relativistic Beaming and Orientation Effects in BL Lacertae Objects ...

    Indian Academy of Sciences (India)

    tation paradigm for high peaked and low-peaked BL Lacs (X-ray and radio selected .... consequences of relativistic beaming and geometric projection were studied in high- ... model. If we assume α = 0 (for synchrotron self-absorbed sources) and β ∼ 1, it can .... for RBL and XBL subsamples at confidence level of ∼ 95.0%.

  4. Properties of optically selected BL Lacertae candidates from the SDSS

    Science.gov (United States)

    Kügler, S. D.; Nilsson, K.; Heidt, J.; Esser, J.; Schultz, T.

    2014-09-01

    Context. Deep optical surveys open the avenue for finding large numbers of BL Lac objects that are hard to identify because they lack the unique properties classifying them as such. While radio or X-ray surveys typically reveal dozens of sources, recent compilations based on optical criteria alone have increased the number of BL Lac candidates considerably. However, these compilations are subject to biases and may contain a substantial number of contaminating sources. Aims: In this paper we extend our analysis of 182 optically selected BL Lac object candidates from the SDSS with respect to an earlier study. The main goal is to determine the number of bona fide BL Lac objects in this sample. Methods: We examine their variability characteristics, determine their broad-band radio-UV spectral energy distributions (SEDs), and search for the presence of a host galaxy. In addition we present new optical spectra for 27 targets with improved signal-to-noise ratio with respect to the SDSS spectra. Results: At least 59% of our targets have shown variability between SDSS DR2 and our observations by more than 0.1-0.27 mag depending on the telescope used. A host galaxy was detected in 36% of our targets. The host galaxy type and luminosities are consistent with earlier studies of BL Lac host galaxies. Simple fits to broad-band SEDs for 104 targets of our sample derived synchrotron peak frequencies between 13.5 ≤ log 10(νpeak) ≤ 16 with a peak at log 10 ~ 14.5. Our new optical spectra do not reveal any new redshift for any of our objects. Thus the sample contains a large number of bona fide BL Lac objects and seems to contain a substantial fraction of intermediate-frequency peaked BL Lacs. Based on observations collected with the NTT on La Silla (Chile) operated by the European Southern Observatory under proposal 082.B-0133.Based on observations collected at the Centro Astronómico Hispano Alemán (CAHA), operated jointly by the Max-Planck-Institut für Astronomie and the

  5. Provider of Services File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The POS file consists of two data files, one for CLIA labs and one for 18 other provider types. The file names are CLIA and OTHER. If downloading the file, note it...

  6. PC Graphic file programing

    International Nuclear Information System (INIS)

    Yang, Jin Seok

    1993-04-01

    This book gives description of basic of graphic knowledge and understanding and realization of graphic file form. The first part deals with graphic with graphic data, store of graphic data and compress of data, programing language such as assembling, stack, compile and link of program and practice and debugging. The next part mentions graphic file form such as Mac paint file, GEM/IMG file, PCX file, GIF file, and TIFF file, consideration of hardware like mono screen driver and color screen driver in high speed, basic conception of dithering and conversion of formality.

  7. Majorana dark matter with B+L gauge symmetry

    Energy Technology Data Exchange (ETDEWEB)

    Chao, Wei [Amherst Center for Fundamental Interactions, Department of Physics,University of Massachusetts-Amherst,Amherst, MA 01003 United States (United States); Center for Advanced Quantum Studies,Department of Physics, Beijing Normal University,Beijing, 100875 (China); Guo, Huai-Ke [Amherst Center for Fundamental Interactions, Department of Physics,University of Massachusetts-Amherst,Amherst, MA 01003 United States (United States); Zhang, Yongchao [Service de Physique Théorique, Université Libre de Bruxelles,Boulevard du Triomphe, CP225, 1050 Brussels (Belgium)

    2017-04-07

    We present a new model that extends the Standard Model (SM) with the local B+L symmetry, and point out that the lightest new fermion ζ, introduced to cancel anomalies and stabilized automatically by the B+L symmetry, can serve as the cold dark matter candidate. We study constraints on the model from Higgs measurements, electroweak precision measurements as well as the relic density and direct detections of the dark matter. Numerical results reveal that the pseudo-vector coupling of ζ with Z and the Yukawa coupling with the SM Higgs are highly constrained by the latest results of LUX, while there are viable parameter space that could satisfy all the constraints and give testable predictions.

  8. Asymmetric Gepner models III. B-L lifting

    Energy Technology Data Exchange (ETDEWEB)

    Gato-Rivera, B. [NIKHEF Theory Group, Kruislaan 409, 1098 SJ Amsterdam (Netherlands); Instituto de Fisica Fundamental, CSIC, Serrano 123, Madrid 28006 (Spain); Schellekens, A.N., E-mail: t58@nikhef.n [NIKHEF Theory Group, Kruislaan 409, 1098 SJ Amsterdam (Netherlands); Instituto de Fisica Fundamental, CSIC, Serrano 123, Madrid 28006 (Spain); IMAPP, Radboud Universiteit, Nijmegen (Netherlands)

    2011-06-21

    In the same spirit as heterotic weight lifting, B-L lifting is a way of replacing the superfluous and ubiquitous U(1){sub B-L} with something else with the same modular properties, but different conformal weights and ground state dimensions. This method works in principle for all variants of (2,2) constructions, such as orbifolds, Calabi-Yau manifolds, free bosons and fermions and Gepner models, since it only modifies the universal SO(10)xE{sub 8} part of the CFT. However, it can only yield chiral spectra if the 'internal' sector of the theory provides a simple current of order 5. Here we apply this new method to Gepner models. Including exceptional invariants, 86 of them have the required order 5 simple current, and 69 of these yield chiral spectra. Three family spectra occur abundantly.

  9. PG 1553 + 11 - A bright optically selected BL Lacertae object

    International Nuclear Information System (INIS)

    Falomo, R.; Treves, A.

    1990-01-01

    A detailed study of the bright optically selected BL Lac object PG 1553 + 11 is presented. UV observations, obtained during a high state of the source, together with simultaneous optical spectrophotometry and near-IR photometry, allow the spectral flux distribution to be examined from 8 x 10 to the 13th to 2.5 x 10 to the 15th Hz. This distribution is compared with that derived from quasi-simultaneous observations obtained when the source was a factor of about 3 fainter. It is found that, in the higher state, the spectrum can be described by two power laws connected by a break at about 10 to the 15th Hz, while in the low state the shape is more complex. The overall spectrum of the object is compared with the average energy distribution of X-ray and radio-selected BL Lac objects, showing that it is closer to the former class. 25 refs

  10. Infrared polarimetry and photometry of BL Lac objects. 2

    Energy Technology Data Exchange (ETDEWEB)

    Impey, C D [Hawaii Univ., Honolulu (USA). Inst. for Astronomy; Brand, P W.J.L. [Edinburgh Univ. (UK). Dept. of Astronomy; Wolstencroft, R D [Royal Observatory, Edinburgh (UK); Williams, P M [United Kingdom Infrared Telescope Unit, Hilo, Hawaii (USA)

    1984-07-15

    Photometry and polarimetry in the JHK wavebands have now been obtained for 25 BL Lac objects. Several new objects have been monitored for periods of up to five days, and accumulated data is sufficient for a statistical analysis of polarization properties. The selection effects operating on this sample are examined first. A power-law spectrum is consistent with the spectra of all but three objects. A number of important new results are reported.

  11. Infrared polarimetry and photometry of BL Lac objects

    International Nuclear Information System (INIS)

    Impey, C.D.; Williams, P.M.

    1984-01-01

    Photometry and polarimetry in the JHK wavebands have now been obtained for 25 BL Lac objects. Several new objects have been monitored for periods of up to five days, and accumulated data is sufficient for a statistical analysis of polarization properties. The selection effects operating on this sample are examined first. A power-law spectrum is consistent with the spectra of all but three objects. A number of important new results are reported. (author)

  12. The BL Lac objects PKS 1144-379

    International Nuclear Information System (INIS)

    Nicolson, G.D.; Glass, I.S.; Feast, M.W.; Andrews, P.J.

    1979-01-01

    The highly variable radio source PKS 1144-379 has been monitored at 13 cm and 6 cm over a period of 3 years. It has been identified with an object whose photographic image is star-like. From infrared photometry, UBVRsub(KC)Isub(KC) photometry and spectroscopy, it is concluded that PKS 1144-379 is a BL Lac object with msub(v) approximately = 16.2. (author)

  13. Optical polarization of high-energy BL Lacertae objects

    Science.gov (United States)

    Hovatta, T.; Lindfors, E.; Blinov, D.; Pavlidou, V.; Nilsson, K.; Kiehlmann, S.; Angelakis, E.; Fallah Ramazani, V.; Liodakis, I.; Myserlis, I.; Panopoulou, G. V.; Pursimo, T.

    2016-12-01

    Context. We investigate the optical polarization properties of high-energy BL Lac objects using data from the RoboPol blazar monitoring program and the Nordic Optical Telescope. Aims: We wish to understand if there are differences between the BL Lac objects that have been detected with the current-generation TeV instruments and those objects that have not yet been detected. Methods: We used a maximum-likelihood method to investigate the optical polarization fraction and its variability in these sources. In order to study the polarization position angle variability, we calculated the time derivative of the electric vector position angle (EVPA) change. We also studied the spread in the Stokes Q/I-U/I plane and rotations in the polarization plane. Results: The mean polarization fraction of the TeV-detected BL Lacs is 5%, while the non-TeV sources show a higher mean polarization fraction of 7%. This difference in polarization fraction disappears when the dilution by the unpolarized light of the host galaxy is accounted for. The TeV sources show somewhat lower fractional polarization variability amplitudes than the non-TeV sources. Also the fraction of sources with a smaller spread in the Q/I-U/I plane and a clumped distribution of points away from the origin, possibly indicating a preferred polarization angle, is larger in the TeV than in the non-TeV sources. These differences between TeV and non-TeV samples seem to arise from differences between intermediate and high spectral peaking sources instead of the TeV detection. When the EVPA variations are studied, the rate of EVPA change is similar in both samples. We detect significant EVPA rotations in both TeV and non-TeV sources, showing that rotations can occur in high spectral peaking BL Lac objects when the monitoring cadence is dense enough. Our simulations show that we cannot exclude a random walk origin for these rotations. Conclusions: These results indicate that there are no intrinsic differences in the

  14. Hydrogeologic Characterization of the U-3bl Collapse Zone

    International Nuclear Information System (INIS)

    NSTec Geotechnical Services

    2006-01-01

    The U-3bl collapse crater was formed by an underground nuclear test in August 1962. This crater and the adjoining U-3ax crater were subsequently developed and used as a bulk low-level radioactive waste disposal cell (U-3ax/bl), which is part of the Area 3 Radioactive Waste Management Site at the Nevada Test Site (NTS). Various investigations have been conducted to assess the hydrogeologic characteristics and properties in the vicinity of the U-3ax/bl waste disposal cell. This report presents data from one of these investigations, conducted in 1996. Also included in this report is a review of pertinent nuclear testing records, which shows that the testing operations and hydrogeologic setting of the U-3ax/bl site were typical for the period and location of testing. Borehole U-3bl-D2 is a 45-degree-angle hole drilled from the edge of the crater under the waste cell to intercept the U-3bl collapse zone, the disturbed alluvium between the crater (surface collapse sink) and the nuclear test cavity. A casing-advance system with an air percussion hammer was used to drill the borehole, and air was used as the drilling fluid. Properties of the U-3bl crater collapse zone were determined from cores collected within the interval, 42.1 to 96.6 meters (138 to 317 feet) below the ground surface. Selected core samples were analyzed for particle density, particle size, bulk density, water retention, hydraulic conductivity, water content, water potential, chloride, carbonate, stable isotopes, and tritium. Physical and hydraulic properties were typical of alluvial valley sediments at the NTS. No visual evidence of preferential pathways for water transport was observed in the core samples. Soil parameters showed no trends with depth. Volumetric water content values ranged from 0.08 to 0.20 cubic meters per cubic meter, and tended to increase with depth. Water-retention relations were typical for soils of similar texture. Water potentials ranged from -1.9 MegaPascals at a depth of 42

  15. Download this PDF file

    African Journals Online (AJOL)

    home

    2016-03-27

    Mar 27, 2016 ... Commercial sugarcane cultivation for the production of table sugar in Ethiopia ... production management practices and application of other sources of nutrients ...... Legendre, B.L. (2001). Sugarcane production handbook.

  16. Download this PDF file

    African Journals Online (AJOL)

    Hp 630 Dual Core

    that lead them to develop polpoosis syndromes namely adenomatous ... Colonic polyps usually present with, rectal bleeding, a mass per rectum, abdominal pain, ... Soravia c, Osconnor Bl, BerkT, Mcloed RS, &cohen Z. Functional outcome of ...

  17. Download this PDF file

    African Journals Online (AJOL)

    Fr. Ikenga

    technologies can gather large amount of individuals' personal information and ... M, (Ilorin) BL; Lecturer, Department of Private and Property Law, Faculty of .... realization by governments and business entities that “information is power and ...

  18. Decay data file based on the ENSDF file

    Energy Technology Data Exchange (ETDEWEB)

    Katakura, J. [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1997-03-01

    A decay data file with the JENDL (Japanese Evaluated Nuclear Data Library) format based on the ENSDF (Evaluated Nuclear Structure Data File) file was produced as a tentative one of special purpose files of JENDL. The problem using the ENSDF file as primary source data of the JENDL decay data file is presented. (author)

  19. Cloning, expression, purification, crystallization and preliminary X-ray analysis of the human RuvBL1–RuvBL2 complex

    International Nuclear Information System (INIS)

    Gorynia, Sabine; Matias, Pedro M.; Bandeiras, Tiago M.; Donner, Peter; Carrondo, Maria Arménia

    2008-01-01

    A truncated variant of the human RuvBL1–RuvBL2 complex was cloned, expressed, purified and crystallised. Synchrotron diffraction data to 4 Å resolution were used to carry out a preliminary crystallographic analysis of the complex. The complex of RuvBL1 and its homologue RuvBL2, two evolutionarily highly conserved eukaryotic proteins belonging to the AAA + (ATPase associated with diverse cellular activities) family of ATPases, was co-expressed in Escherichia coli. For crystallization purposes, the flexible domains II of RuvBL1 and RuvBL2 were truncated. The truncated RuvBL1–RuvBL2 complex was crystallized using the hanging-drop vapour-diffusion method at 293 K. The crystals were hexagonal-shaped plates and belonged to either the orthorhombic space group C222 1 , with unit-cell parameters a = 111.4, b = 188.0, c = 243.4 Å and six monomers in the asymmetric unit, or the monoclinic space group P2 1 , with unit-cell parameters a = 109.2, b = 243.4, c = 109.3 Å, β = 118.7° and 12 monomers in the asymmetric unit. The crystal structure could be solved by molecular replacement in both possible space groups and the solutions obtained showed that the complex forms a dodecamer

  20. UPIN Group File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Group Unique Physician Identifier Number (UPIN) File is the business entity file that contains the group practice UPIN and descriptive information. It does NOT...

  1. X-ray studies of BL Lacertae objects

    International Nuclear Information System (INIS)

    Madejski, G.M.

    1986-01-01

    This thesis presents spectral x-ray data for BL Lac objects observed by the IPC and MPC aboard the Einstein Observatory and interprets that data in a context of their overall radiation spectra using synchrotron and synchrotron self-Compton models. The objects considered are: OJ 287, PKS 0735 + 178, I Zw 186, PKS 0548-322, Mkn 180, BL Lacertae, PKS 2155-304, H 0414-009 and H 0323 + 022. X-ray spectra of BL Lac objects are well described by a power law model with a low energy cutoff due to absorption within the own Galaxy. The best fit values of the energy spectral index α in the IPC (0.2-4.0 keV) band range from 0.73 to 2.35, with a mean of 1.2 and rms spread of 0.51. No single, universal index can fit the spectra of all objects. For all objects except PKS 0735 + 178, the x-ray spectrum is an extrapolation of the infrared/optical UV spectrum; in PKS 0735 + 178, the x-ray spectrum lies significantly below such an extrapolation. The overall electromagnetic distribution in those objects is interpreted as arising due to the synchrotron process in at least two spatial regions, with sizes respectively ∼10 18 cm for the radio component and ∼10 16 cm for the optical component. In objects where the x-ray spectrum lies on the extrapolation of the infrared-optical-ultraviolet spectrum, the x-ray emission is interpreted also to be due to the synchrotron process

  2. On the independence of axioms in BL and MTL

    Czech Academy of Sciences Publication Activity Database

    Chvalovský, Karel

    2012-01-01

    Roč. 197, 16 June (2012), s. 123-129 ISSN 0165-0114 R&D Projects: GA ČR GEICC/08/E018; GA ČR GD401/09/H007 Grant - others:GA UK(CZ) 73109/2009 Institutional research plan: CEZ:AV0Z10300504 Keywords : non-classical logics * basic fuzzy logic (BL) * monoidal t-norm based logic (MTL) * Hilbert-style calculi * independence of axioms Subject RIV: BA - General Mathematics Impact factor: 1.749, year: 2012

  3. PCF File Format.

    Energy Technology Data Exchange (ETDEWEB)

    Thoreson, Gregory G [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-08-01

    PCF files are binary files designed to contain gamma spectra and neutron count rates from radiation sensors. It is the native format for the GAmma Detector Response and Analysis Software (GADRAS) package [1]. It can contain multiple spectra and information about each spectrum such as energy calibration. This document outlines the format of the file that would allow one to write a computer program to parse and write such files.

  4. A File Archival System

    Science.gov (United States)

    Fanselow, J. L.; Vavrus, J. L.

    1984-01-01

    ARCH, file archival system for DEC VAX, provides for easy offline storage and retrieval of arbitrary files on DEC VAX system. System designed to eliminate situations that tie up disk space and lead to confusion when different programers develop different versions of same programs and associated files.

  5. Text File Comparator

    Science.gov (United States)

    Kotler, R. S.

    1983-01-01

    File Comparator program IFCOMP, is text file comparator for IBM OS/VScompatable systems. IFCOMP accepts as input two text files and produces listing of differences in pseudo-update form. IFCOMP is very useful in monitoring changes made to software at the source code level.

  6. Opening of material analysis beamline (BL-5) at NewSUBARU for industrial enterprises

    International Nuclear Information System (INIS)

    Hasegawa, Takayuki; Uemura, Masaharu; Tsurui, Takafumi; Shimizu, Masayoshi; Amemiya, Kenta; Fukushima, Sei; Ohta, Toshiaki; Motoyama, Muneyuki; Kanda, Kazuhiro

    2010-01-01

    A material analysis beamline for the industrial enterprises' use was completed at BL-5 of NewSUBARU synchrotron radiation facility in University of Hyogo in March 2008. BL-5 consists of two branch lines, one is a double crystal monochromator beamline (BL-5A) for the use in the higher-energy region (1300-4000 eV) and the other is a varied line spacing plane grating (VLSPG) monochromator beamline (BL-5B) for the use in the lower-energy region (50-1300 eV). These two branch lines can be operated simultaneously. BL-5 covers the soft X-ray region from 50 to 4000 eV and X-ray absorption spectrum can be measured with a high-energy resolution. The XAFS measurements in the total electron yield (TEY) and fluorescence yield (FLY) can be performed at BL-5A and BL-5B. In addition, the XPS spectra can be measured at BL-5B. BL-5 will be managed and maintained by the Synchrotron Analysis L.L.C. (SALLC), which is composed of the industrial companies, in cooperation with the staffs of the Laboratory of Advanced Science and Technology for Industry in University of Hyogo. Industrial users can be assisted by staffs of SALLC in the measurement at BL-5. (author)

  7. BL.-SL. *balnā „brazdas; oda“

    Directory of Open Access Journals (Sweden)

    Vincas Urbutis

    2011-12-01

    Full Text Available BL.-SL. *balnā "SPLINT; HAUT"ZusammenfassungSl. *bolna „Splint (weiche saftige Holzschicht unter der Rinde; dünnes Häutchen, Haut“ (vgl. russ. болона́ „Splint“, čech. blána „dünnes Häutchen; Splint; (ačech. Haut, Fell“ hatte früher seine genaue formelle und semantische Entsprechung lit. *balna, von dem das Verb balnóti „schlagen, prügeln“ gebildet ist, dessen frühere Bedeutung „schälen, abhäuten“ war, vgl. nubalnóti „abscheuern, abhäuten, Haut abreißen“. Das läßt annehmen bl.—sl. *balnā „Splint; Haut“ — eine substantivierte feminine Form von dem Adjektiv, dessen Fortsetzung lit. bálnas (bal̃nas,-à „weiß, mit dem weißen Rücken (von einem Ochsen oder einer Kuh“ ist.

  8. Accretion disk emission from a BL Lacertae object

    International Nuclear Information System (INIS)

    Wandel, A.; Urry, C.M.

    1991-01-01

    It is suggested here that the UV and X-ray emission of BL Lac objects may originate in an accretion disk. Using detailed calculations of accretion disk spectra, the best-measured ultraviolet and soft X-ray spectra of the BL Lac object PKS 2155-304 are fitted, and the mass and accretion rate required is determined. The ultraviolet through soft X-ray continuum is well fitted by the spectrum of an accretion disk, but near-Eddington accretion rates are required to produce the soft X-ray excess. A hot disk or corona could Comptonize soft photons from the cool disk and produce the observed power-law spectrum in the 1-10 keV range. The dynamic time scale in the disk regions that contribute most of the observed ultraviolet and soft X-ray photons are consistent with the respective time scales for intensity variations observed in these two wave bands; the mass derived from fitting the continuum spectrum is consistent with the limit derived from the fastest hard X-ray variability. 37 refs

  9. Opacity and noninear effects on theoretical BL Herculis models

    International Nuclear Information System (INIS)

    Hodson, S.W.; Cox, A.N.; King, D.S.

    1982-01-01

    Linear and nonlinear pulsation models for BL Herculis variables have been constructed to investigate the resonance which seems to occur when the ratio of the second overtone (Pi 2 ) to fundamental (Pi 0 ) radial periods is near 0.5. This resonance is shown to affect the shapes of the light and velocity curves and produce bumps on either ascending or descending light just as far classical Cepheids. Linear theory predicts the resonance to occur at periods between 1.7 and 3.0 days for 0.55 M/sub sun/ and between 2.1 and 4.0 days for 0.75 M/sub sun/ stars at the red and blue edges, respectively, of the stability strip. These periods are rather independent of the composition and opacity tables. However, observations show the resonance to be about 1.7 days for all BL Her variables by noticing that the bump phase switches from descending to ascending light as the period increases. Nonlinear calculations indicate that the linear theory predictions of Pi 2 /Pi 0 are not reliable just at Pi 2 /Pi 0 = 0.5, and the predicted resonance occurs always at the proper period as observed

  10. Title list of documents made publicly available. Documents from October-December 1978 for Dockets 50-334 through STN 50-597

    International Nuclear Information System (INIS)

    1978-01-01

    This special edition of the Title List contains Docket 50 material from 1978 that has not appeared in previous issues of the Title List. The documents in this supplement are indexed by personal author, corporate source, and report number. The listings of this material on the domestic licensing of production and utilization facilities, divided into the categories used for filing and searching in the NRC Public Document Room, are presented

  11. Polarization burst in the BL Lac object AO 0235 + 164

    Energy Technology Data Exchange (ETDEWEB)

    Impey, C D; Brand, P W.J.L. [Edinburgh Univ. (UK). Dept. of Astronomy; Tapia, S [Steward Observatory, Tucson, AZ (USA)

    1982-01-01

    Simultaneous infrared and optical polarimetry and photometry have been obtained for AO 0235 + 164 covering a five night period. The object underwent a polarization burst during which the 2.2 ..mu..m polarization rose from 17.5 to 28.7 per cent and fell again to 14.9 per cent. At its peak the degree of optical polarization was 43.9 per cent, the highest linear polarization observed in a BL Lac object. The data show the degree of polarization to increase towards shorter wavelengths, and the effect is inconsistent with either dilution by a galactic component or simple one-component synchrotron models. The large changes in polarization are not accompanied by large changes in flux, a result which is difficult to explain using conventional models of these objects. Other implications of the luminosity, polarization and variability are discussed.

  12. Methamphetamine protects against MPTP neurotoxicity in C57BL mice.

    Science.gov (United States)

    Sziráki, I; Kardos, V; Patthy, M; Pátfalusi, M; Budai, G

    1994-01-14

    Methamphetamine (5 mg/kg) administered 30 min prior to each injection with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (3 x 30 mg/kg, at 24 h intervals) prevents the reduction of striatal levels of dopamine and its metabolites in C57BL mice. Methamphetamine and amphetamine inhibit the uptake of 1-methyl-4-phenylpyridinium (MPP+) by striatal synaptosomes of rats. A 30-min post-treatment with methamphetamine or amphetamine also prevents the MPTP-induced dopamine depletion, suggesting that their protective effect is related to the blockade of MPP+ uptake into dopaminergic neurons. Since amphetamine and methamphetamine are themselves neurotoxins at higher doses, this work demonstrated the protection against the actions of one neurotoxin by the administration of another.

  13. Final environmental statement related to the proposed manufacture of floating nuclear power plants: (Docket No. STN 50-437): Part 2, A generic environmental statement considering the siting and operation of floating nuclear power plants

    International Nuclear Information System (INIS)

    1976-09-01

    The proposed action is the issuance of a manufacturing license to Offshore Power Systems for the startup and operation of a proposed manufacturing facility located at Blount Island, Jacksonville, Florida (Docket No. STN 50-437). No nuclear fuel will be handled or stored at the manufacturing site. The plants will be fueled after they have been towed to and moored within protected basins at specific locations designated by the purchaser and after an operating license has been issued by the Nuclear Regulatory Commission. Each nuclear generating plant, mounted on a floating platform, has a net capacity of 1150 MWe. This energy is provided by a pressurized water reactor steam supply system consisting of a Westinghouse four-loop 3425-MWt unit with an ice-condenser containment system. When one or more of these units is located within a single breakwater, the installation is designated an offshore power station. 226 figs., 95 tabs

  14. Source Reference File

    Data.gov (United States)

    Social Security Administration — This file contains a national set of names and contact information for doctors, hospitals, clinics, and other facilities (known collectively as sources) from which...

  15. Patient Assessment File (PAF)

    Data.gov (United States)

    Department of Veterans Affairs — The Patient Assessment File (PAF) database compiles the results of the Patient Assessment Instrument (PAI) questionnaire filled out for intermediate care Veterans...

  16. RRB Earnings File (RRBERN)

    Data.gov (United States)

    Social Security Administration — RRBERN contains records for all beneficiaries on the RRB's PSSVES file who's SSNs are validated through the SVES processing. Validated output is processed through...

  17. The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer

    Science.gov (United States)

    Munkley, Jennifer; Oltean, Sebastian; Vodák, Daniel; Wilson, Brian T.; Livermore, Karen E.; Zhou, Yan; Star, Eleanor; Floros, Vasileios I.; Johannessen, Bjarne; Knight, Bridget; McCullagh, Paul; McGrath, John; Crundwell, Malcolm; Skotheim, Rolf I.; Robson, Craig N.; Leung, Hing Y.; Harries, Lorna W.; Rajan, Prabhakar; Mills, Ian G.; Elliott, David J.

    2015-01-01

    Patterns of glycosylation are important in cancer, but the molecular mechanisms that drive changes are often poorly understood. The androgen receptor drives prostate cancer (PCa) development and progression to lethal metastatic castration-resistant disease. Here we used RNA-Seq coupled with bioinformatic analyses of androgen-receptor (AR) binding sites and clinical PCa expression array data to identify ST6GalNAc1 as a direct and rapidly activated target gene of the AR in PCa cells. ST6GalNAc1 encodes a sialytransferase that catalyses formation of the cancer-associated sialyl-Tn antigen (sTn), which we find is also induced by androgen exposure. Androgens induce expression of a novel splice variant of the ST6GalNAc1 protein in PCa cells. This splice variant encodes a shorter protein isoform that is still fully functional as a sialyltransferase and able to induce expression of the sTn-antigen. Surprisingly, given its high expression in tumours, stable expression of ST6GalNAc1 in PCa cells reduced formation of stable tumours in mice, reduced cell adhesion and induced a switch towards a more mesenchymal-like cell phenotype in vitro. ST6GalNAc1 has a dynamic expression pattern in clinical datasets, being significantly up-regulated in primary prostate carcinoma but relatively down-regulated in established metastatic tissue. ST6GalNAc1 is frequently upregulated concurrently with another important glycosylation enzyme GCNT1 previously associated with prostate cancer progression and implicated in Sialyl Lewis X antigen synthesis. Together our data establishes an androgen-dependent mechanism for sTn antigen expression in PCa, and are consistent with a general role for the androgen receptor in driving important coordinate changes to the glycoproteome during PCa progression. PMID:26452038

  18. Blæksprutter beskyttes på niveau med hvirveldyr

    DEFF Research Database (Denmark)

    Alstrup, Aage Kristian Olsen

    2015-01-01

    beskyttede. Et af de bedst studerede hvirvelløse dyr er blæksprutten, navnlig Octopus vulgaris. Vores viden om nerveimpulsen udbredelse stammer netop fra blæksprutter, da de har meget store nerveceller, der let lader sig undersøge. De to forskere fik faktisk Nobelprisen i 1963 for opdagelsen. Siden da har...

  19. Flat radio-spectrum galaxies and BL Lacs I. Core properties

    NARCIS (Netherlands)

    Dennett-Thorpe, J; Marcha, MJ

    This paper concerns the relationship of BL Lacs and flat-spectrum weak emission-line galaxies. We compare the weak emission-line galaxies and the BL Lacs in a sample of 57 flat-spectrum objects (Marcha et al. 1996), using high-frequency radio and non-thermal optical flux densities, spectral indices

  20. Gene expression profiling in response to the histone deacetylase inhibitor BL1521 in neuroblastoma

    International Nuclear Information System (INIS)

    Ruijter, Annemieke J.M. de; Meinsma, Rutger J.; Bosma, Peter; Kemp, Stephan; Caron, Huib N.; Kuilenburg, Andre B.P. van

    2005-01-01

    Neuroblastoma is a childhood tumor with a poor survival in advanced stage disease despite intensive chemotherapeutic regimes. The new histone deacetylase (HDAC) inhibitor BL1521 has shown promising results in neuroblastoma. Inhibition of HDAC resulted in a decrease in proliferation and metabolic activity, induction of apoptosis and differentiation of neuroblastoma cells. In order to elucidate the mechanism mediating the effects of BL1521 on neuroblastoma cells, we investigated the gene expression profile of an MYCN single copy (SKNAS) and an MYCN amplified (IMR32) neuroblastoma cell line after treatment with BL1521 using the Affymetrix oligonucleotide array U133A. An altered expression of 255 genes was observed in both neuroblastoma cell lines. The majority of these genes were involved in gene expression, cellular metabolism, and cell signaling. We observed changes in the expression of vital genes belonging to the cell cycle (cyclin D1 and CDK4) and apoptosis (BNIP3, BID, and BCL2) pathway in response to BL1521. The expression of 37 genes was altered by both BL1521 and Trichostatin A, which could indicate a common gene set regulated by different HDAC inhibitors. BL1521 treatment changed the expression of a number of MYCN-associated genes. Several genes in the Wnt and the Delta/Notch pathways were changed in response to BL1521 treatment, suggesting that BL1521 is able to induce the differentiation of neuroblastoma cells into a more mature phenotype

  1. 76 FR 62092 - Filing Procedures

    Science.gov (United States)

    2011-10-06

    ... INTERNATIONAL TRADE COMMISSION Filing Procedures AGENCY: International Trade Commission. ACTION: Notice of issuance of Handbook on Filing Procedures. SUMMARY: The United States International Trade Commission (``Commission'') is issuing a Handbook on Filing Procedures to replace its Handbook on Electronic...

  2. The effects of electromagnetic fields on B16-BL6 cells are dependent on their spatial and temporal character.

    Science.gov (United States)

    Buckner, Carly A; Buckner, Alison L; Koren, Stan A; Persinger, Michael A; Lafrenie, Robert M

    2017-04-01

    Exposure to low intensity, low frequency electromagnetic fields (EMF) has effects on several biological systems. Spatiotemporal characteristics of these EMFs are critical. The effect of several complex EMF patterns on the proliferation of B16-BL6 mouse melanoma cells was tested. Exposure to one of these patterns, the Thomas-EMF, inhibited cell proliferation and promoted calcium uptake. The Thomas-EMF is coded from a digital-to-analog file comprised of 849 points, which provides power to solenoids and can be set to alter timing, intensity, and duration of variable EMF. Setting the point duration to 3 ms generated a time-varying EMF pattern which began at 25 Hz and slowed to 6 Hz over a 2.5 s repeat. Exposing B16-BL6 cells to Thomas-EMF set to 3 ms for 1 h/day inhibited cell proliferation by 40% after 5 days, while setting the point duration to 1, 2, 4, or 5 ms had no effect on cell proliferation. Similarly, exposing cells to Thomas-EMF set to 3 ms promoted a three-fold increase in calcium uptake after 1 h, while the other timings had no effect. Exposure to Thomas-EMF for as short as 15 min/day slowed cell proliferation, but exposure for 1 h/day was optimal. This corresponded to the effect on calcium uptake where uptake was detected after 15 min exposure and was maximal by 1 h of treatment. Studies show that the specific spatiotemporal character of EMF is critical in mediating their biological activities. Bioelectromagnetics. 38:165-174, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  3. Safety evaluation report related to the operation of Wolf Creek Generating Staton, Unit No. 1, (Docket No. STN 50-482). Supplement No. 4

    International Nuclear Information System (INIS)

    1983-12-01

    Supplement No. 4 to the Safety Evaluation Report related to the operation of the Wolf Creek Generating Station, Unit No. 1 updates the information contained in the Safety Evaluation Report, dated April 1982 and Supplements 1, 2, and 3, dated August 1982, June 1983 and August, 1983, respectively. Supplement No. 4 addresses open issues, confirmatory items and addresses Board Notifications. The Safety Evaluation and its supplements pertain to the application for a license to operate the Wolf Creek Generating Station, Unit No. 1 filed by Kansas Gas and Electric Company on February 19, 1980. The Construction Permit No. CPPR-147 was issued on May 17, 1977

  4. Safety Evaluation Report related to the operation of Shearon Harris Nuclear Power Plant, Unit No. 1 (Docket No. STN 50-400). Supplement No. 4

    International Nuclear Information System (INIS)

    1986-10-01

    This report, Supplement No. 4 to the Safety Evaluation Report for the application filed by the Carolina Power and Light Company and North Carolina Eastern Municipal Power Agency (the applicants) for a license to operate the Shearon Harris Nuclear Power Plant Unit 1 (Docket No. 50-400), has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission. This supplement reports the status of certain items that had not been resolved at the time of publication of the Safety Evaluation Report and Supplement Nos. 1, 2, and 3

  5. Safety evaluation report related to the operation of Shearon Harris Nuclear Power Plant, Unit No. 1 (Docket No. STN 50-400). Supplement No. 1

    International Nuclear Information System (INIS)

    1984-06-01

    This report, Supplement No. 1 to the Safety Evaluation Report for the application filed by the Carolina Power and Light Company and North Carolina Eastern Municipal Power Agency (the applicant) for license to operate the Shearon Harris Nuclear Power Plant Unit 1 (Docket No. 50-400), has been prepared by the Office of Nuclear Reactor Regulation of the U.S. Nuclear Regulatory Commission. This supplement reports the status of certain items that had not been resolved at the time of publication of the Safety Evaluation Report

  6. Safety Evaluation Report related to the operation of Shearon Harris Nuclear Power Plant, Unit No. 1 (Docket No. STN 50-400). Supplement No. 2

    International Nuclear Information System (INIS)

    1985-06-01

    This report, Supplement No. 2 to the Safety Evaluation Report for the application filed by the Carolina Power and Light Company and North Carolina Eastern Municipal Power Agency (the applicants) for a license to operate the Shearon Harris Nuclear Power Plant Unit 1 (Docket No. 50-400), has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission. This supplement reports the status of certain items that had not been resolved at the time of publication of the Safety Evaluation Report and Supplement No. 1

  7. Safety Evaluation Report related to the operation of Shearon Harris Nuclear Power Plant, Unit No. 1 (Docket No. STN 50-400). Supplement No. 3

    International Nuclear Information System (INIS)

    1986-05-01

    This report, Supplement No. 3 to the Safety Evaluation Report for the application filed by the Carolina Power and Light Company and North Carolina Eastern Municipal Power Agency (the applicants) for a license to operate the Shearon Harris Nuclear Power Plant Unit 1 (Docket No. 50-400), has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission. This supplement reports the status of certain items that had not been resolved at the time of publication of the Safety Evaluation Report and Supplement Nos. 1 and 2

  8. Safety evaluation report related to the construction of Skagit/Hanford Nuclear Project, Units 1 and 2. Docket Nos. STN 50-522 and 50-523

    International Nuclear Information System (INIS)

    1982-12-01

    Supplement 3 to the Safety Evaluation Report for the application filed by Puget Sound Power and Light Company on behalf of itself, the Pacific Power and Light Company, The Washington Water Power Company, and the Portland General Electric Company for construction permits to build the Skagit/Hanford Nuclear Project has been issued by the Office of Nuclear Reactor Regulation of the United States Nuclear Regulatory Commission. This supplement is an evaluation of the site relocation amendment to the Preliminary Safety Analysis Report. The proposed site has been relocated from Skagit County, Washington, to the Department of Energy's Hanford Reservation

  9. Broad band energy distribution of UV-bright BL Lac objects

    International Nuclear Information System (INIS)

    Maraschi, L.; Tanzi, E.G.; Treves, A.

    1984-01-01

    IUE satellite data in the 1200-2000 and 1900-3200 A intervals of BL Lac objects are analyzed in terms of two discernible groups. A total of 25 BL Lac objects were observed, with differences between groups displayed in terms of the power slope of the energy of the UV emissions, i.e., slopes of 1 and 2. Comparisons of the spectra with those of quasars showed that quasars have a small spectral index in the 1000-6000 A band and no correlation exists between the spectral index and UV flux of the BL Lac objects. The comparisons underscore the lack of a thermal component for BL Lac objects. Steep spectral components in both BL Lac objects and highly polarized quasars emissions could both be due to synchrotron emission. Compton scattering of relativistic electrons off synchrotron photons could produce the X ray emissions. 44 references

  10. Broad band energy distribution of UV-bright BL Lac objects

    Energy Technology Data Exchange (ETDEWEB)

    Maraschi, L.; Tanzi, E.G.; Treves, A.

    1984-01-01

    IUE satellite data in the 1200-2000 and 1900-3200 A intervals of BL Lac objects are analyzed in terms of two discernible groups. A total of 25 BL Lac objects were observed, with differences between groups displayed in terms of the power slope of the energy of the UV emissions, i.e., slopes of 1 and 2. Comparisons of the spectra with those of quasars showed that quasars have a small spectral index in the 1000-6000 A band and no correlation exists between the spectral index and UV flux of the BL Lac objects. The comparisons underscore the lack of a thermal component for BL Lac objects. Steep spectral components in both BL Lac objects and highly polarized quasars emissions could both be due to synchrotron emission. Compton scattering of relativistic electrons off synchrotron photons could produce the X ray emissions. 44 references.

  11. The REX survey as a Tool to Test the Beaming Model for BL Lacs

    Science.gov (United States)

    Caccianiga, A.; della Ceca, R.; Gioia, I. M.; Maccacaro, T.; Wolter, A.

    We present the preliminary properties of the BL Lacs discovered in the REX survey (Caccianiga et al. 1998). In particular, we discuss a few sources with optical spectral properties ``intermediate'' between those of BL Lacs and those of elliptical galaxies. These objects could harbour weak (in the optical band) sources of non-thermal continuum in their nuclei and, if confirmed, they could represent the faint tail of the BL Lac population. The existence of such ``weak'' BL Lacs is matter of discussion in recent literature (e.g. Marcha et al. 1996) and could lead to a revision of the defining criteria of a BL Lac and, consequently, of their cosmological and statistical properties.

  12. FHEO Filed Cases

    Data.gov (United States)

    Department of Housing and Urban Development — The dataset is a list of all the Title VIII fair housing cases filed by FHEO from 1/1/2007 - 12/31/2012 including the case number, case name, filing date, state and...

  13. Evolutionary behaviour of AGN: Investigations on BL Lac objects and Seyfert II galaxies

    Science.gov (United States)

    Beckmann, V.

    2000-12-01

    The evolution and nature of AGN is still one of the enigmatic questions in astrophysics. While large and complete Quasar samples are available, special classes of AGN, like BL Lac objects and Seyfert II galaxies, are still rare objects. In this work I present two new AGN samples. The first one is the HRX-BL Lac survey, resulting in a sample of X-ray selected BL Lac objects. This sample results from 223 BL Lac candidates based on a correlation of X-ray sources with radio sources. The identification of this sample is 98% complete. 77 objects have been identified as BL Lac objects and form the HRX-BL Lac complete sample, the largest homogeneous sample of BL Lac objects existing today. For this sample, redshifts are now known for 62 objects (81 %). In total I present 101 BL Lac objects in the enlarged HRX-BL Lac survey, for which redshift information is available for 84 objects. During the HRX-BL Lac survey I found several objects of special interest. 1ES 1517+656 turned out to be the brightest known BL Lac object in the universe. 1ES 0927+500 could be the first BL Lac object with a line detected in the X-ray region. RX J1211+2242 is probably the the counterpart of the up to now unidentified gamma-ray source 3EG J1212+2304. Additionally I present seven candidates for ultra high frequency peaked BL Lac objects. RX J1054+3855 and RX J1153+3517 are rare high redshift X-ray bright QSO or accreting binary systems with huge magnetic fields. For the BL Lac objects I suggest an unified scenario in which giant elliptical galaxies, formed by merging events of spiral galaxies at z > 2, start as powerful, radio dominated BL Lacs. As the jet gets less powerful, the BL Lacs start to get more X-ray dominated, showing less total luminosities (for z definition to objects with a calcium break up to 40%, but do not support for the HBL the idea of allowing emission lines in the spectra of BL Lac galaxies. A way to find high redshift BL Lac objects might be the identification of faint X

  14. Current status of BL-2B at photon factory

    Energy Technology Data Exchange (ETDEWEB)

    Nambu, Akira, E-mail: akia.nambu.tw@hitachi.com; Ueda, Kazuhiro [Central Research Laboratory, Hitachi, ltd. 1-280 Higashi-Koigakubo, Kokubunji, Tokyo, 185-8601 (Japan); Horiba, Koji; Tsuchiya, Kimichika; Kumigashira, Hiroshi; Amemiya, Kenta [KEK-PF 1-1 Oho, Tsukuba, Ibaraki, 305-0801 (Japan)

    2016-07-27

    A new soft x-ray beamline BL-2B at Photon Factory of High Energy Accelerator Research Organization (KEK-PF) covers energy range from vacuum ultraviolet (30 eV) to soft x-ray (4000 eV). This wide energy range could be achieved by employing two undulators and two monochromators. Two different energy range undulators were installed tandem to a 9-meter straight section of PF storage ring. The 1{sup st} undulator is for VUV (30 eV) to SX (280 eV), while the other one is for SX (280 eV) to HX (4000 eV). It is also necessary to be equipped with two different monochrometors for energy above and under 2000 eV; grating monochrometor and double crystal monochrometor. One of the main purposes of this bemaline is spectroscopic study of light elements contained in several functional materials. The beamline is designed for photoemission spectroscopy (PES), X-ray absorption fine structure (XAFS) and other types of experiments. The performance of the new beamline is reported and typical examples of its application to material science are demonstrated.

  15. Toyota beamline (BL33XU) at SPring-8

    Energy Technology Data Exchange (ETDEWEB)

    Nonaka, T., E-mail: nonaka@mosk.tytlabs.co.jp; Dohmae, K.; Hayashi, Y.; Yamaguchi, S.; Nagai, Y.; Hirose, Y. [Toyota Central R& D Labs., Inc., 41-1 Nagakute Aichi 480-1192 Japan (Japan); Araki, T. [Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE UK (United Kingdom); Tanaka, T.; Kitamura, H. [RIKEN Harima Institute 1-1-1 Koto Sayo, Hyogo 679-5148 (Japan); Uruga, T.; Yamazaki, H.; Yumoto, H.; Ohashi, H.; Goto, S. [JASRI/SPring-8 1-1-1 Koto Sayo, Hyogo 679-5148 (Japan)

    2016-07-27

    The Toyota beamline (BL33XU) at SPring-8 is an undulator beamline developed to assist in the study of various automotive-related materials. The light source is a tapered in-vacuum undulator that provides a variable energy band width as well as a high brilliance X-ray beam. Two different optical arrangements are available: Optics 1 and Optics 2. Optics 1 is dedicated to time-resolved X-ray absorption spectroscopy (XAFS), and consists of two channel-cut crystal monochromators and four water-cooled flat Si mirrors. The Si(111) and Si(220) monochromator crystals cover an energy range of 4.0–46.0 keV and are driven by high-speed AC servo motors. These monochromators, in conjunction with the tapered undulator, enable high-quality XAFS data acquisition with a temporal resolution of 10 ms. Optics 2 is optimized for X-ray diffraction, scattering and imaging and includes a recently installed double crystal monochromator, two water-cooled flat Si mirrors and Kirkpatrick-Baez (KB) focusing mirrors. The monochromator incorporates parallel mounted Si(111) and Si(311) crystals and covers an energy range of 4.5–70 keV. The beamline provides two experimental stations: Exp. Hutch 2 and Exp. Hutch 3. The gas supply system and mass spectrometers installed in Exp. Hutch 2 allow in-operando measurements under various atmospheres. The scanning three-dimensional X-ray diffraction (scanning 3DXRD) microscopy instrumentation developed and installed in Exp. Hutch 3 enables non-destructive orientation and stress mapping of 1 mm-thick steel specimens using a high energy microbeam.

  16. Toyota beamline (BL33XU) at SPring-8

    International Nuclear Information System (INIS)

    Nonaka, T.; Dohmae, K.; Hayashi, Y.; Yamaguchi, S.; Nagai, Y.; Hirose, Y.; Araki, T.; Tanaka, T.; Kitamura, H.; Uruga, T.; Yamazaki, H.; Yumoto, H.; Ohashi, H.; Goto, S.

    2016-01-01

    The Toyota beamline (BL33XU) at SPring-8 is an undulator beamline developed to assist in the study of various automotive-related materials. The light source is a tapered in-vacuum undulator that provides a variable energy band width as well as a high brilliance X-ray beam. Two different optical arrangements are available: Optics 1 and Optics 2. Optics 1 is dedicated to time-resolved X-ray absorption spectroscopy (XAFS), and consists of two channel-cut crystal monochromators and four water-cooled flat Si mirrors. The Si(111) and Si(220) monochromator crystals cover an energy range of 4.0–46.0 keV and are driven by high-speed AC servo motors. These monochromators, in conjunction with the tapered undulator, enable high-quality XAFS data acquisition with a temporal resolution of 10 ms. Optics 2 is optimized for X-ray diffraction, scattering and imaging and includes a recently installed double crystal monochromator, two water-cooled flat Si mirrors and Kirkpatrick-Baez (KB) focusing mirrors. The monochromator incorporates parallel mounted Si(111) and Si(311) crystals and covers an energy range of 4.5–70 keV. The beamline provides two experimental stations: Exp. Hutch 2 and Exp. Hutch 3. The gas supply system and mass spectrometers installed in Exp. Hutch 2 allow in-operando measurements under various atmospheres. The scanning three-dimensional X-ray diffraction (scanning 3DXRD) microscopy instrumentation developed and installed in Exp. Hutch 3 enables non-destructive orientation and stress mapping of 1 mm-thick steel specimens using a high energy microbeam.

  17. Antiparasitic activities of acridone alkaloids from Swinglea glutinosa (Bl.) Merr

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Djalma A.P. dos; Vieira, Paulo C; Silva, M. Fatima das G.F. da; Fernandes, Joao B [Universidade Federal de Sao Carlos, SP (Brazil). Dept. de Quimica; Rattray, Lauren; Croft, Simon L [London School of Hygiene and Tropical Medicine, London (United Kingdom). Dept. of Infectious and Tropical Diseases

    2009-07-01

    Eleven acridone alkaloids isolated from Swinglea glutinosa (Bl.) Merr. were examined for in vitro activity against chloroquine-sensitive Plasmodium falciparum 3D7, Trypanosoma brucei rhodesiense STIB900 and Leishmania donovani L82. An assay with KB cells was developed in order to compare in vitro toxicity of alkaloids with the selective action on the parasites. Nine of the compounds had IC{sub 50} values ranging from 0.3 to 11.6 {mu}M against P. falciparum. In contrast, a small number of compounds showed significant activity against T. brucei rhodesiense and none had activity against L. donovani. Among the alkaloids three had IC{sub 50} < 1.0 {mu}M against P. falciparum, whereas against T. b. rhodesiense five had IC{sub 50} < 10 {mu}M. The characterization of the acridone alkaloids, 1,3,5-trihydroxy-4-methoxy-10-methyl-2,8-bis(3-methylbut-2-enyl)acridin-9 (10H)-one (1), 2,3-dihydro-4,9-dihydroxy-2-(2-hydroxypropan-2-yl)-11-methoxy-10-methylfuro [3,2-b] acridin-5(10H)-one (2) and 3,4-dihydro-3,5,8-trihydroxy-6-methoxy-2,2,7-trimethyl-2Hpyrano[ 2,3-a]acridin-12(7H)-one (3), is discussed, as well as the structure-activity relationship of all compounds assayed. Isolation and spectral data of alkaloids 1-3 are described for the first time although their cytotoxicities to cancer cells have been described before. (author)

  18. On the Redshift of TeV BL Lac Objects

    Energy Technology Data Exchange (ETDEWEB)

    Paiano, Simona; Falomo, Renato [INAF, Osservatorio Astronomico di Padova, Vicolo dell’Osservatorio 5 I-35122 Padova (PD) (Italy); Landoni, Marco; Righi, Chiara [INAF, Osservatorio Astronomico di Brera, Via E. Bianchi 46 I-23807 Merate (Italy); Treves, Aldo [Università degli Studi dell’Insubria, Via Valleggio 11 I-22100 Como (Italy); Scarpa, Riccardo [Instituto de Astrofisica de Canarias, C/O Via Lactea, s/n E38205—La Laguna (Tenerife) (Spain)

    2017-03-10

    We report results of a spectroscopic campaign carried out at the 10 m Gran Telescopio Canarias for a sample of 22 BL Lac objects detected (or candidates) at TeV energies, aiming to determine or constrain their redshift. This is of fundamental importance for the interpretation of their emission models and for population studies and is also mandatory for studying the interaction of high-energy photons with the extragalactic background light using TeV sources. Optical spectra with high signal-to-noise ratios in the range 4250–10000 Å were obtained to search for faint emission or absorption lines from both the host galaxy and the nucleus. We determine a new redshift for PKS 1424+240 ( z = 0.604) and a tentative one for 1ES 0033+595 ( z = 0.467). We are able to set new spectroscopic redshift lower limits for three other sources on the basis of Mg ii and Ca ii intervening absorption features: BZB J1243+3627 ( z > 0.483), BZB J1540+8155 ( z > 0.672), and BZB 0J2323+4210 ( z > 0.267). We confirm previous redshift estimates for four blazars: S3 0218+357 ( z = 0.944), 1ES 1215+303 ( z = 0.129), W Comae ( z = 0.102), and MS 1221.8+2452 ( z = 0.218). For the remaining targets, in seven cases (S2 0109+22, 3C 66A, VER J0521+211, S4 0954+65, BZB J1120+4214, S3 1227+25, BZB J2323+4210), we do not validate the proposed redshift. Finally, for all sources of still-unknown redshift, we set a lower limit based on the minimum equivalent width of absorption features expected from the host galaxy.

  19. Download this PDF file

    African Journals Online (AJOL)

    5,. May. 1923, p. 287. ISouth African Military Schools) p 287. CGS Box 231, File 31/0/2. .... One gains the impression that the sphere .... tions, Anthropology, Sociology and Man Manage- ment. ... of the word, possesses personality and initiative,.

  20. MMLEADS Public Use File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare-Medicaid Linked Enrollee Analytic Data Source (MMLEADS) Public Use File (PUF) contains demographic, enrollment, condition prevalence, utilization, and...

  1. Hospital Service Area File

    Data.gov (United States)

    U.S. Department of Health & Human Services — This file is derived from the calendar year inpatient claims data. The records contain number of discharges, length of stay, and total charges summarized by provider...

  2. Patient Treatment File (PTF)

    Data.gov (United States)

    Department of Veterans Affairs — This database is part of the National Medical Information System (NMIS). The Patient Treatment File (PTF) contains a record for each inpatient care episode provided...

  3. USEEIO Satellite Files

    Data.gov (United States)

    U.S. Environmental Protection Agency — These files contain the environmental data as particular emissions or resources associated with a BEA sectors that are used in the USEEIO model. They are organized...

  4. Provider of Services File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The POS file contains data on characteristics of hospitals and other types of healthcare facilities, including the name and address of the facility and the type of...

  5. Download this PDF file

    African Journals Online (AJOL)

    countries quite a number of distance education institutions and programmes are more likely to be ... The Open University of Tanzania (OUT), (Ministry of Higher Education, Science and ..... (1991) Comic Relief Funding file. BAI, London, 1st ...

  6. Emilie Bláhová (13. 6. 1931 - 9. 10. 2016)

    Czech Academy of Sciences Publication Activity Database

    Chromá, Martina

    2017-01-01

    Roč. 140, 1-2 (2017), s. 275-277 ISSN 0024-4457 Institutional support: RVO:68378017 Keywords : Bláhová, Emilie * obituary * paleoslavistics Subject RIV: AI - Linguistics OBOR OECD: Specific languages

  7. Computing Optical Variable Periods of BL Lac Object S5 0716+ 714 ...

    Indian Academy of Sciences (India)

    Computing Optical Variable Periods of BL Lac Object S5 0716+ 714 ... The study of long-term periodical variation is an important way to get the charac- ... continuous Fourier transform together, define a window function, and finally obtain.

  8. OS IMPACTOS NO ATIVO IMOBILIZADO DA UNIVERSIDADE FEDERAL DE JUIZ DE FORA (UFJF E SUAS DECORRÊNCIAS PARA O CONTROLE GERENCIAL INSTITUCIONAL A PARTIR DA IMPLANTAÇÃO DA PORTARIA CONJUNTA SPU-STN N. 703/2014

    Directory of Open Access Journals (Sweden)

    Maria Simoni Nascimento Soncin

    2017-01-01

    Full Text Available A atualização do valor contábil dos imóveis ao valor de mercado busca evidenciar a capacidade destes ativos em gerar benefícios econômicos e de prestar serviços de qualidade à sociedade. Objetivou-se com essa pesquisa apresentar a atualização, proposta na Portaria Conjunta SPU/STN N. 703/2014, dos bens imóveis de uso educacional, do campus da Universidade Federal de Juiz de Fora, para averiguar os impactos desse procedimento no subgrupo do ativo Imobilizado e suas decorrências para o controle gerencial. O método utilizado como estratégia de pesquisa foi o estudo de caso, realizado na UFJF, por meio de atualizações dos valores registrados no SPIUnet e de análises horizontal e vertical, no balanço patrimonial, nos anos de 2014 e 2015. Os resultados da pesquisa demonstraram que o impacto no imobilizado é relevante, representando um acréscimo real de 34,36% no patrimônio imobiliário desta Universidade. E, que este procedimento poderá auxiliar o controle gerencial, pois fortalece a credibilidade da informação e a transparência dos atos da gestão pública.   The updating of the book value of real estate to market value seeks to highlight the ability of these assets to generate economic benefits and to provide quality services to society. The objective of this research was to present the update, proposed in Joint Ordinance SPU / STN No. 703/2014, of real estate for educational use, at the campus of the Federal University of Juiz de Fora, to investigate the impacts of this procedure on the subgroup of assets Fixed assets and their consequences for management control. The method used as a research strategy was the case study carried out at the UFJF, through updates of the values recorded in SPIUnet and of horizontal and vertical analyzes in the balance sheet, in the years 2014 and 2015. The results of the research demonstrated that The impact on property, plant and equipment is significant, representing a real increase of 34

  9. JENDL Dosimetry File

    International Nuclear Information System (INIS)

    Nakazawa, Masaharu; Iguchi, Tetsuo; Kobayashi, Katsuhei; Iwasaki, Shin; Sakurai, Kiyoshi; Ikeda, Yujiro; Nakagawa, Tsuneo.

    1992-03-01

    The JENDL Dosimetry File based on JENDL-3 was compiled and integral tests of cross section data were performed by the Dosimetry Integral Test Working Group of the Japanese Nuclear Data Committee. Data stored in the JENDL Dosimetry File are the cross sections and their covariance data for 61 reactions. The cross sections were mainly taken from JENDL-3 and the covariances from IRDF-85. For some reactions, data were adopted from other evaluated data files. The data are given in the neutron energy region below 20 MeV in both of point-wise and group-wise files in the ENDF-5 format. In order to confirm reliability of the data, several integral tests were carried out; comparison with the data in IRDF-85 and average cross sections measured in fission neutron fields, fast reactor spectra, DT neutron fields and Li(d, n) neutron fields. As a result, it has been found that the JENDL Dosimetry File gives better results than IRDF-85 but there are some problems to be improved in future. The contents of the JENDL Dosimetry File and the results of the integral tests are described in this report. All of the dosimetry cross sections are shown in a graphical form. (author) 76 refs

  10. JENDL Dosimetry File

    Energy Technology Data Exchange (ETDEWEB)

    Nakazawa, Masaharu; Iguchi, Tetsuo [Tokyo Univ. (Japan). Faculty of Engineering; Kobayashi, Katsuhei [Kyoto Univ., Kumatori, Osaka (Japan). Research Reactor Inst.; Iwasaki, Shin [Tohoku Univ., Sendai (Japan). Faculty of Engineering; Sakurai, Kiyoshi; Ikeda, Yujior; Nakagawa, Tsuneo [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1992-03-15

    The JENDL Dosimetry File based on JENDL-3 was compiled and integral tests of cross section data were performed by the Dosimetry Integral Test Working Group of the Japanese Nuclear Data Committee. Data stored in the JENDL Dosimetry File are the cross sections and their covariance data for 61 reactions. The cross sections were mainly taken from JENDL-3 and the covariances from IRDF-85. For some reactions, data were adopted from other evaluated data files. The data are given in the neutron energy region below 20 MeV in both of point-wise and group-wise files in the ENDF-5 format. In order to confirm reliability of the data, several integral tests were carried out; comparison with the data in IRDF-85 and average cross sections measured in fission neutron fields, fast reactor spectra, DT neutron fields and Li(d,n) neutron fields. As a result, it has been found that the JENDL Dosimetry File gives better results than IRDF-85 but there are some problems to be improved in future. The contents of the JENDL Dosimetry File and the results of the integral tests are described in this report. All of the dosimetry cross sections are shown in a graphical form.

  11. Inhibition of B16-BL6 melanoma growth in mice by methionine-enkephalin.

    Science.gov (United States)

    Murgo, A J

    1985-08-01

    The antitumor effect of methionine-enkephalin [( Met]enkephalin) was demonstrated in C57BL/6J mice inoculated with B16-BL6 melanoma cells. Local subcutaneous tumor growth was inhibited with a 50-micrograms dose daily for 7 or 14 days. The antitumor effect of [Met]enkephalin was inhibited by the administration of the opioid receptor antagonist naloxone. Naloxone alone had no significant effect on tumor growth.

  12. Multi-Wave Luminosity of High-Synchrotron-Peaked TeV BL Lacs ...

    Indian Academy of Sciences (India)

    LIR, Lγ) in the radio, near-infrared and γ-ray wave bands for HSP. TeV BL Lacs. The results show that there are significant intrinsic cor- relations between LR and Lγ and between LIR and Lγ in all states. (high/average/low), and suggest that for HSP TeV BL Lacs, the Syn- chrotron Self-Compton radiation (SSC) is the main ...

  13. Renewal-anomalous-heterogeneous files

    International Nuclear Information System (INIS)

    Flomenbom, Ophir

    2010-01-01

    Renewal-anomalous-heterogeneous files are solved. A simple file is made of Brownian hard spheres that diffuse stochastically in an effective 1D channel. Generally, Brownian files are heterogeneous: the spheres' diffusion coefficients are distributed and the initial spheres' density is non-uniform. In renewal-anomalous files, the distribution of waiting times for individual jumps is not exponential as in Brownian files, yet obeys: ψ α (t)∼t -1-α , 0 2 >, obeys, 2 >∼ 2 > nrml α , where 2 > nrml is the MSD in the corresponding Brownian file. This scaling is an outcome of an exact relation (derived here) connecting probability density functions of Brownian files and renewal-anomalous files. It is also shown that non-renewal-anomalous files are slower than the corresponding renewal ones.

  14. Clinical Chemistry Reference Intervals for C57BL/6J, C57BL/6N, and C3HeB/FeJ Mice (Mus musculus).

    Science.gov (United States)

    Otto, Gordon P; Rathkolb, Birgit; Oestereicher, Manuela A; Lengger, Christoph J; Moerth, Corinna; Micklich, Kateryna; Fuchs, Helmut; Gailus-Durner, Valérie; Wolf, Eckhard; Hrabě de Angelis, Martin

    2016-01-01

    Although various mouse inbred strains are widely used to investigate disease mechanisms and to establish new therapeutic strategies, sex-specific reference intervals for laboratory diagnostic analytes that are generated from large numbers of animals have been unavailable. In this retrospective study, we screened data from more than 12,000 mice phenotyped in the German Mouse Clinic from January 2006 through June 2014 and selected animals with the genetic background of C57BL/6J, C57BL/6N, or C3HeB/FeJ. In addition, we distinguished between the C57BL/6NTac substrain and C57BL/6N mice received from other vendors. The corresponding data sets of electrolytes (sodium, potassium, calcium, chloride, inorganic phosphate), lipids (cholesterol, triglyceride), and enzyme activities (ALT, AST, ALP, α-amylase) and urea, albumin, and total protein levels were analyzed. Significant effects of age and sex on these analytes were identified, and strain- or substrain- and sex-specific reference intervals for 90- to 135-d-old mice were calculated. In addition, we include an overview of the literature that reports clinical chemistry values for wild-type mice of different strains. Our results support researchers interpreting clinical chemistry values from various mouse mutants and corresponding wild-type controls based on the examined strains and substrains.

  15. The B-L phase transition. Implications for cosmology and neutrinos

    International Nuclear Information System (INIS)

    Schmitz, Kai

    2012-07-01

    We investigate the possibility that the hot thermal phase of the early universe is ignited in consequence of the B-L phase transition, which represents the cosmological realization of the spontaneous breaking of the Abelian gauge symmetry associated with B-L, the difference between baryon number B and lepton number L. Prior to the B-L phase transition, the universe experiences a stage of hybrid inflation. Towards the end of inflation, the false vacuum of unbroken B-L symmetry decays, which entails tachyonic preheating as well as the production of cosmic strings. Observational constraints on this scenario require the B-L phase transition to take place at the scale of grand unification. The dynamics of the B-L breaking Higgs field and the B-L gauge degrees of freedom, in combination with thermal processes, generate an abundance of heavy (s)neutrinos. These (s)neutrinos decay into radiation, thereby reheating the universe, generating the baryon asymmetry of the universe and setting the stage for the thermal production of gravitinos. The B-L phase transition along with the (s)neutrino-driven reheating process hence represents an intriguing and testable mechanism to generate the initial conditions of the hot early universe. We study the B-L phase transition in the full supersymmetric Abelian Higgs model, for which we derive and discuss the Lagrangian in arbitrary and unitary gauge. As for the subsequent reheating process, we formulate the complete set of Boltzmann equations, the solutions of which enable us to give a detailed and time-resolved description of the evolution of all particle abundances during reheating. Assuming the gravitino to be the lightest superparticle (LSP), the requirement of consistency between hybrid inflation, leptogenesis and gravitino dark matter implies relations between neutrino parameters and superparticle masses, in particular a lower bound on the gravitino mass of 10GeV. As an alternative to gravitino dark matter, we consider the case of

  16. The B-L phase transition. Implications for cosmology and neutrinos

    Energy Technology Data Exchange (ETDEWEB)

    Schmitz, Kai

    2012-07-15

    We investigate the possibility that the hot thermal phase of the early universe is ignited in consequence of the B-L phase transition, which represents the cosmological realization of the spontaneous breaking of the Abelian gauge symmetry associated with B-L, the difference between baryon number B and lepton number L. Prior to the B-L phase transition, the universe experiences a stage of hybrid inflation. Towards the end of inflation, the false vacuum of unbroken B-L symmetry decays, which entails tachyonic preheating as well as the production of cosmic strings. Observational constraints on this scenario require the B-L phase transition to take place at the scale of grand unification. The dynamics of the B-L breaking Higgs field and the B-L gauge degrees of freedom, in combination with thermal processes, generate an abundance of heavy (s)neutrinos. These (s)neutrinos decay into radiation, thereby reheating the universe, generating the baryon asymmetry of the universe and setting the stage for the thermal production of gravitinos. The B-L phase transition along with the (s)neutrino-driven reheating process hence represents an intriguing and testable mechanism to generate the initial conditions of the hot early universe. We study the B-L phase transition in the full supersymmetric Abelian Higgs model, for which we derive and discuss the Lagrangian in arbitrary and unitary gauge. As for the subsequent reheating process, we formulate the complete set of Boltzmann equations, the solutions of which enable us to give a detailed and time-resolved description of the evolution of all particle abundances during reheating. Assuming the gravitino to be the lightest superparticle (LSP), the requirement of consistency between hybrid inflation, leptogenesis and gravitino dark matter implies relations between neutrino parameters and superparticle masses, in particular a lower bound on the gravitino mass of 10GeV. As an alternative to gravitino dark matter, we consider the case of

  17. Final environmental statement related to the proposed manufacture of floating nuclear power plants by Offshore Power Systems: (Docket No. STN 50-437): Part 2, A generic environmental statement considering the siting and operation of floating nuclear power plants

    International Nuclear Information System (INIS)

    1976-09-01

    The proposed action is the issuance of a manufacturing license to Offshore Power Systems for the startup and operation of a proposed manufacturing facility located at Blount Island, Jacksonville, Florida (Docket No. STN 50-437). No nuclear fuel will be handled or stored at the manufacturing site. The plants will be fueled after they have been towed to and moored within protected basins at specific locations designated by the purchaser and after an operating license has been issued by the Nuclear Regulatory Commission. Each nuclear generating plant, mounted on a floating platform, has a net capacity of 1150 MWe. This energy is provided by a pressurized water reactor steam supply system consisting of a Westinghouse four-loop 3425-MWt unit with an ice-condenser containment system. When one or more of these units is located within a single breakwater, the installation is designated an offshore power station. Volume 2 contains the siting criteria, regulations, effects of construction, effects of operation, and a safety analysis. 2 figs., 2 tabs

  18. Probiotic Strain Lactobacillus casei BL23 Prevents Colitis-Associated Colorectal Cancer

    Directory of Open Access Journals (Sweden)

    Elsa Jacouton

    2017-11-01

    Full Text Available The gut microbiota plays a major role in intestinal health, and an imbalance in its composition can lead to chronic gut inflammation and a predisposition to developing colorectal cancer (CRC. Currently, the use of probiotic bacteria represents an emerging alternative to treat and prevent cancer. Moreover, consumption of these beneficial bacteria may also favorably modulate the composition of the gut microbiota, which has been described in several studies to play an important role in CRC carcinogenesis. In this context, the aim of this study was to assess the protective effect of oral treatment with Lactobacillus casei BL23, a probiotic strain well known for its anti-inflammatory and anticancer properties. First, CRC was induced in C57BL6 mice by a single intraperitoneal injection with azoxymethane (8 mg/kg, followed by four courses of dextran sodium sulfate (2.5% in drinking water that were separated by an adjustable recovery period. At the time of sacrifice (day 46, tumor incidence, histological scores, and epithelial proliferation were determined in colon samples. Our results show that L. casei BL23 significantly protected mice against CRC development; specifically, L. casei BL23 treatment reduced histological scores and proliferative index values. In addition, our analysis revealed that L. casei BL23 had an immunomodulatory effect, mediated through the downregulation of the IL-22 cytokine, and an antiproliferative effect, mediated through the upregulation of caspase-7, caspase-9, and Bik. Finally, L. casei BL23 treatment tended to counterbalance CRC-induced dysbiosis in mice, as demonstrated by an analysis of fecal microbiota. Altogether our results demonstrate the high potential of L. casei BL23 for the development of new, probiotic-based strategies to fight CRC.

  19. Thermostable Bacteriocin BL8 from Bacillus licheniformis isolated from marine sediment.

    Science.gov (United States)

    Smitha, S; Bhat, S G

    2013-03-01

    To isolate and characterize bacteriocin, BL8, from the bacteria identified as Bacillus licheniformis from marine environment. One-hundred and twelve bacterial isolates from sediment and water samples collected off the coast of Cochin, India, were screened for antibacterial activity. Strain BTHT8, identified as Bacillus licheniformis, inhibited the growth of Gram-positive test organisms. The active component labelled as bacteriocin BL8 was partially purified by ammonium sulphate fractionation and was subjected to glycine SDS-PAGE. The band exhibiting antimicrobial activity was electroeluted and analysed using MALDI-TOF mass spectrometry, and the molecular mass was determined as 1.4 kDa. N-terminal amino acid sequencing of BL8 gave a 13 amino acid sequence stretch. Bacteriocin BL8 was stable even after boiling at 100 °C for 30 min and over a wide pH range of 1-12. A novel, pH-tolerant and thermostable bacteriocin BL8, active against the tested Gram-positive bacteria, was isolated from Bacillus licheniformis. This study reports a stable, low molecular weight bacteriocin from Bacillus licheniformis. This bacteriocin can be used to address two important applications: as a therapeutic agent and as a biopreservative in food processing industry. © 2012 The Society for Applied Microbiology.

  20. Effect of ketamine on exploratory behaviour in BALB/C and C57BL/6 mice.

    Science.gov (United States)

    Akillioglu, Kubra; Melik, Emine Babar; Melik, Enver; Boga, Ayper

    2012-01-01

    In this study, we evaluated the effect of ketamine on exploratory locomotion behaviours in the Balb/c and C57BL/6 strains of mice, which differ in their locomotion behaviours. Intraperitoneal administration of ketamine at three different doses (1, 5 or 10 mg/kg, 0.1 ml/10 gr body weight) was performed on adult male Balb/c and C57BL/6 mice. The same volume of saline was applied to the control group. The open-field and elevated plus maze apparatus were used to evaluate exploratory locomotion. In the open-field test, Balb/c mice less spend time in the centre of the field and was decreased locomotor activity compared to C57BL/6 mice (pmice at 10 mg/kg dose caused an increase in locomotor activity and an increase in the amount of time spent in the centre in the open-field test, compared to the control group (pmice, ketamine treatment (1 and 10 mg/kg) decreased locomotor activity (pmice, the three different doses of ketamine application each caused a decrease in the frequency of centre crossing (pmice compared to C57BL/6 mice (pmice at 10 mg/kg dose caused an increase in the open-arm activity (pmice (pmice. In contrast, a subanaesthetic dose of ketamine decreased exploratory locomotion in C57BL/6 mice. In conclusion, hereditary factors may play an important role in ketamine-induced responses. Copyright © 2011 Elsevier Inc. All rights reserved.

  1. Phenotypic stability of B16-BL6 melanoma exposed to low levels of tyrosine and phenylalanine.

    Science.gov (United States)

    Elstad, C A; Meadows, G G

    1990-01-01

    We previously demonstrated that tyrosine (Tyr) and phenylalanine (Phe) restriction suppresses metastatic heterogeneity of B16-BL6 (BL6) melanoma and selects for tumor variants with decreased metastatic potential. In this study, we investigate stability of this Tyr- and Phe-modulated tumor phenotype by sequentially transplanting BL6 in vivo into mice fed Low Tyr and Phe Diet. Metastatic potential of BL6 is suppressed after one subcutaneous passage. Suppression is unlikely to result from inhibition of tumor growth, since growth in vitro is significantly increased. The metastatic potential of the Tyr- and Phe-modulated tumor is unstable after in vivo passage, and lung colonizing ability is regenerated after ten in vivo passages. Conversely, the antimetastatic effect of Tyr and Phe restriction is stable after prolonged in vitro passage. The metastatic potential of tumors from mice fed Normal Diet is unstable after long-term in vitro culture. Sensitivity to adriamycin of BL6 from mice fed Low Tyr and Phe Diet is increased and is not altered by change in metastatic potential.

  2. Formalizing a hierarchical file system

    NARCIS (Netherlands)

    Hesselink, Wim H.; Lali, Muhammad Ikram

    An abstract file system is defined here as a partial function from (absolute) paths to data. Such a file system determines the set of valid paths. It allows the file system to be read and written at a valid path, and it allows the system to be modified by the Unix operations for creation, removal,

  3. Formalizing a Hierarchical File System

    NARCIS (Netherlands)

    Hesselink, Wim H.; Lali, M.I.

    2009-01-01

    In this note, we define an abstract file system as a partial function from (absolute) paths to data. Such a file system determines the set of valid paths. It allows the file system to be read and written at a valid path, and it allows the system to be modified by the Unix operations for removal

  4. Long term file migration. Part I: file reference patterns

    International Nuclear Information System (INIS)

    Smith, A.J.

    1978-08-01

    In most large computer installations, files are moved between on-line disk and mass storage (tape, integrated mass storage device) either automatically by the system or specifically at the direction of the user. This is the first of two papers which study the selection of algorithms for the automatic migration of files between mass storage and disk. The use of the text editor data sets at the Stanford Linear Accelerator Center (SLAC) computer installation is examined through the analysis of thirteen months of file reference data. Most files are used very few times. Of those that are used sufficiently frequently that their reference patterns may be examined, about a third show declining rates of reference during their lifetime; of the remainder, very few (about 5%) show correlated interreference intervals, and interreference intervals (in days) appear to be more skewed than would occur with the Bernoulli process. Thus, about two-thirds of all sufficiently active files appear to be referenced as a renewal process with a skewed interreference distribution. A large number of other file reference statistics (file lifetimes, interference distributions, moments, means, number of uses/file, file sizes, file rates of reference, etc.) are computed and presented. The results are applied in the following paper to the development and comparative evaluation of file migration algorithms. 17 figures, 13 tables

  5. Accessing files in an Internet: The Jade file system

    Science.gov (United States)

    Peterson, Larry L.; Rao, Herman C.

    1991-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  6. Accessing files in an internet - The Jade file system

    Science.gov (United States)

    Rao, Herman C.; Peterson, Larry L.

    1993-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  7. Download this PDF file

    African Journals Online (AJOL)

    1- is gifts' ta5ie" in elist fig'equitable' fees distilition s ... O'." & 1 25; 33i) re...) C SS Sati ri. Southerri'Stillah diffigFiles'f actities s % -- - , a v. & ' " St - a s fit . . . fiji ſti i ...

  8. Challenging Ubiquitous Inverted Files

    NARCIS (Netherlands)

    de Vries, A.P.

    2000-01-01

    Stand-alone ranking systems based on highly optimized inverted file structures are generally considered ‘the’ solution for building search engines. Observing various developments in software and hardware, we argue however that IR research faces a complex engineering problem in the quest for more

  9. The Global File System

    Science.gov (United States)

    Soltis, Steven R.; Ruwart, Thomas M.; OKeefe, Matthew T.

    1996-01-01

    The global file system (GFS) is a prototype design for a distributed file system in which cluster nodes physically share storage devices connected via a network-like fiber channel. Networks and network-attached storage devices have advanced to a level of performance and extensibility so that the previous disadvantages of shared disk architectures are no longer valid. This shared storage architecture attempts to exploit the sophistication of storage device technologies whereas a server architecture diminishes a device's role to that of a simple component. GFS distributes the file system responsibilities across processing nodes, storage across the devices, and file system resources across the entire storage pool. GFS caches data on the storage devices instead of the main memories of the machines. Consistency is established by using a locking mechanism maintained by the storage devices to facilitate atomic read-modify-write operations. The locking mechanism is being prototyped in the Silicon Graphics IRIX operating system and is accessed using standard Unix commands and modules.

  10. Download this PDF file

    African Journals Online (AJOL)

    AJNS WEBMASTERS

    Incidence is higher in the elderly, about 58 per 100,000 per year. Diagnosis of CSDH is still .... in the other two patients was not stated in the case file. Evacuation of the Subdural .... Personal experience in 39 patients. Br J of Neurosurg. 2003 ...

  11. File System Virtual Appliances

    Science.gov (United States)

    2010-05-01

    4 KB of data is read or written, data is copied back and forth using trampoline buffers — pages that are shared during proxy initialization — because...in 2008. CIO Magazine. 104 · File system virtual appliances [64] Megiddo, N. and Modha, D. S. 2003. ARC: A Self-Tuning, Low Over- head Replacement

  12. ASCA and Contemporaneous Ground-based Observations of the BL Lacertae Objects 1749+096 and 2200+420 (BL Lac)

    International Nuclear Information System (INIS)

    Sambruna, R.M.; Ghisellini, G.; Hooper, E.; Kollgaard, R.I.; Pesce, J.E.; Urry, C.M.

    1999-01-01

    We present ASCA observations of the radio-selected BL Lacertae objects 1749+096 (z=0.32) and 2200+420 (BL Lac, z=0.069) performed in 1995 September and November, respectively. The ASCA spectra of both sources can be described as a first approximation by a power law with photon index Γ∼2. This is flatter than for most X-ray endash selected BL Lacs observed with ASCA, in agreement with the predictions of current blazar unification models. While 1749+096 exhibits tentative evidence for spectral flattening at low energies, a concave continuum is detected for 2200+420: the steep low-energy component is consistent with the high-energy tail of the synchrotron emission responsible for the longer wavelengths, while the harder tail at higher energies is the onset of the Compton component. The two BL Lacs were observed with ground-based telescopes from radio to TeV energies contemporaneously with ASCA. The spectral energy distributions are consistent with synchrotron self-Compton emission from a single homogeneous region shortward of the IR/optical wavelengths, with a second component in the radio domain related to a more extended emission region. For 2200+420, comparing the 1995 November state with the optical/GeV flare of 1997 July, we find that models requiring inverse Compton scattering of external photons provide a viable mechanism for the production of the highest (GeV) energies during the flare. In particular, an increase of the external radiation density and of the power injected in the jet can reproduce the flat γ-ray continuum observed in 1997 July. A directly testable prediction of this model is that the line luminosity in 2200+420 should vary shortly after (∼1 month) a nonthermal synchrotron flare. copyright copyright 1999. The American Astronomical Society

  13. Multi-Band Intra-Night Optical Variability of BL Lacertae

    Directory of Open Access Journals (Sweden)

    Haritma Gaur

    2017-12-01

    Full Text Available We monitored BL Lacertae frequently during 2014–2016 when it was generally in a high state. We searched for intra-day variability for 43 nights using quasi-simultaneous measurements in the B, V, R, and I bands (totaling 143 light curves; the typical sampling interval was about eight minutes. On hour-like timescales, BL Lac exhibited significant variations during 13 nights in various optical bands. Significant spectral variations are seen during most of these nights such that the optical spectrum becomes bluer when brighter. The amplitude of variability is usually greater for longer observations but is lower when BL Lac is brighter. No evidence for periodicities or characteristic variability time-scales in the light curves was found. The color variations are mildly chromatic on long timescales.

  14. Spectroscopy of optically selected BL Lac objects and their γ-ray emission

    Energy Technology Data Exchange (ETDEWEB)

    Sandrinelli, A.; Treves, A.; Farina, E. P.; Landoni, M. [Università degli Studi dell' Insubria, Via Valleggio 11, I-22100 Como (Italy); Falomo, R. [INAF-Osservatorio Astronomico di Padova, Vicolo dell Osservatorio 5, I-35122 Padova (Italy); Foschini, L.; Sbarufatti, B., E-mail: angela.sandrinelli@brera.inaf.it [INAF-Osservatorio Astronomico di Brera, Via Emilio Bianchi 46, I-23807 Merate (Italy)

    2013-12-01

    We present Very Large Telescope optical spectroscopy of nine BL Lac objects of unknown redshift belonging to the list of optically selected radio-loud BL Lac candidates. We explore their spectroscopic properties and possible link with gamma-ray emission. From the new observations we determine the redshifts of four objects from faint emission lines or from absorption features of their host galaxies. In three cases we find narrow intervening absorptions from which a lower limit to the redshift is inferred. For the remaining two featureless sources, lower limits to the redshift are deduced from the absence of spectral lines. A search for γ counterpart emission shows that six out of the nine candidates are Fermi γ-ray emitters and we find two new detections. Our analysis suggests that most of the BL Lac objects still lacking redshift information are most likely located at high redshifts.

  15. The Radio-optical Spectra of BL Lacs and Possible Relatives

    Science.gov (United States)

    Dennett-Thorpe, J.

    I consider the suggestion that, in a complete sample of flat-spectrum radio sources with available optical spectra (Marcha et al 1996), the strong emission line objects, or those with passive elliptical spectra are close relatives of the BL Lacs. New observations at four frequencies from 8 to 43GHz are presented, together with evidence for radio variability. Combined with other radio and optical data from the literature, we are able to construct the non-thermal SEDs and use these to address the questions: are the optically passive objects potentially `unrecognised' BL Lacs (either intrinsically weak and/or hidden by starlight)? What is the relationship between the surprising number of strong emission-line objects and the BL Lacs?

  16. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Science.gov (United States)

    2010-01-01

    ... a facsimile machine or by electronic mail if the reporting entity is not required to file..., including electronic reporting entities, may use the Commission's website's on-line program to file 48-hour... the reporting entity is not required to file electronically in accordance with 11 CFR 104.18. [67 FR...

  17. Evaluated neutronic file for indium

    International Nuclear Information System (INIS)

    Smith, A.B.; Chiba, S.; Smith, D.L.; Meadows, J.W.; Guenther, P.T.; Lawson, R.D.; Howerton, R.J.

    1990-01-01

    A comprehensive evaluated neutronic data file for elemental indium is documented. This file, extending from 10 -5 eV to 20 MeV, is presented in the ENDF/B-VI format, and contains all neutron-induced processes necessary for the vast majority of neutronic applications. In addition, an evaluation of the 115 In(n,n') 116m In dosimetry reaction is presented as a separate file. Attention is given in quantitative values, with corresponding uncertainty information. These files have been submitted for consideration as a part of the ENDF/B-VI national evaluated-file system. 144 refs., 10 figs., 4 tabs

  18. Abnormally banded chromosomal regions in doxorubicin-resistant B16-BL6 murine melanoma cells.

    Science.gov (United States)

    Slovak, M L; Hoeltge, G A; Ganapathi, R

    1986-08-01

    B16-BL6 murine melanoma cells were selected for cytogenetic evaluation during the stepwise development of increasing resistance in vitro to the antitumor antibiotic, doxorubicin (DOX). Karyotypic studies demonstrated extensive heteroploidy with both numerical and structural abnormalities which were not present in the parental DOX-sensitive B16-BL6 cells. Trypsin-Giemsa banding revealed the presence of several marker chromosomes containing abnormally banding regions (ABRs) in the 44-fold B16-BL6 DOX-resistant subline. These ABRs appeared to be more homogeneously staining at the higher DOX concentrations. Length measurements (ABR index) in seven banded metaphases indicated a direct correlation with increasing DOX concentration. When the DOX-resistant cells were grown in drug-free medium for 1 yr, the drug-resistant phenotype gradually declined in parallel with the level of resistance and the ABR index. DOX-induced cytogenetic damage examined by sister chromatid exchange methodology in parental B16-BL6 cells indicated a linear sister chromatid exchange:DOX dose-response relationship. However, after continuous treatment of parental B16-BL6 cells with DOX (0.01 microgram/ml) for 30 days, sister chromatid exchange scores were found to return to base-line values. The B16-BL6 resistant cells demonstrated a cross-resistant phenotype with N-trifluoroacetyladriamycin-14-valerate, actinomycin D, and the Vinca alkaloids but not with 1-beta-D-arabinofuranosylcytosine. The results suggest that ABR-containing chromosomes in DOX-resistant sublines may represent cytogenetic alterations of specific amplified genes involved in the expression of DOX resistance. Further studies are required to identify and define the possible gene products and to correlate their relationship to the cytotoxic action of doxorubicin.

  19. Synchrotron total reflection X-ray fluorescence at BL-16 microfocus beamline of Indus-2

    Energy Technology Data Exchange (ETDEWEB)

    Tiwari, M. K., E-mail: mktiwari@rrcat.gov.in; Singh, A. K., E-mail: mktiwari@rrcat.gov.in; Das, Gangadhar, E-mail: mktiwari@rrcat.gov.in; Chowdhury, Anupam, E-mail: mktiwari@rrcat.gov.in; Lodha, G. S., E-mail: mktiwari@rrcat.gov.in [Indus Synchrotrons Utilisation Division, Raja Ramanna Centre for Advanced Technology, Indore-452013 (India)

    2014-04-24

    Determination of ultra trace elements is important in many disciplines both in basic and applied sciences. Numerous applications show their importance in medical science, environmental science, materials science, food processing and semiconductor industries and in maintaining the quality control of ultra pure chemicals and reagents. We report commissioning of a synchrotron based total reflection x-ray fluorescence (TXRF) facility on the BL-16 microfocus beamline of Indus-2. This paper describes the performance of the BL-16 TXRF spectrometer and the detailed description of its capabilities through examples of measured results.

  20. (M,N-Soft Intersection BL-Algebras and Their Congruences

    Directory of Open Access Journals (Sweden)

    Xueling Ma

    2014-01-01

    Full Text Available The purpose of this paper is to give a foundation for providing a new soft algebraic tool in considering many problems containing uncertainties. In order to provide these new soft algebraic structures, we discuss a new soft set-(M, N-soft intersection set, which is a generalization of soft intersection sets. We introduce the concepts of (M, N-SI filters of BL-algebras and establish some characterizations. Especially, (M, N-soft congruences in BL-algebras are concerned.

  1. The Sedentary Multi-Frequency Survey. I. Statistical Identification and Cosmological Properties of HBL BL Lacs

    OpenAIRE

    Giommi, P.; Menna, M. T.; Padovani, P.

    1999-01-01

    We have assembled a multi-frequency database by cross-correlating the NVSS catalog of radio sources with the RASSBSC list of soft X-ray sources, obtaining optical magnitude estimates from the Palomar and UK Schmidt surveys as provided by the APM and COSMOS on-line services. By exploiting the nearly unique broad-band properties of High-Energy Peaked (HBL) BL Lacs we have statistically identified a sample of 218 objects that is expected to include about 85% of BL Lacs and that is therefore seve...

  2. Studies on emerging radiation leukemia virus variants in C57BL/Ka mice

    International Nuclear Information System (INIS)

    Rassart, E.; Shang, M.; Boie, Y.; Jolicoeur, P.

    1986-01-01

    To analyze the emergence of radiation leukemia virus (RadLV) variants in primary X-ray-induced C57BL/Ka thymoma and to identify the virus responsible for the very high leukemogenic potential of passaged Kaplan strain BL/VL3 preparation, we cloned several primary and passaged ecotropic RadLV infectious genomes. By restriction analysis, we found that BL/VL3 cells harbor three related but different ecotropic RadLVs. Their restriction map differs significantly from those of primary RadLVs. Hybridization analysis also indicated that BL/VL3 and primary RadLVs differ in their p15E and long terminal repeat (LTR) regions. The LTR sequence of primary weakly leukemogenic RadLV has only one change, a C-rich sequence, generating a 6-base-pair direct repeat just in front of the promotor. The LTR of the primary nonleukemogenic RadLV only showed few base changes, mainly clustered in R and U5. The LTR from a moderately leukemogenic passaged BL/VL3 RadLV had conserved the C-rich sequence and acquired a 43-base-pair direct repeat in U3 and several other point mutations, small insertions, and deletions scattered in U3, R, and U5. All cloned primary RadLVs were fibrotropic, and some were weakly leukemogenic. All cloned BL/VL3 RadLVs were thymotropic and nonfibrotropic. The block of their replication was found to be after the synthesis of unintegrated linear and supercoiled viral DNA. Most of the BL/VL3 RadLVs were moderately leukemogenic, and one (V-13) was highly leukemogenic, being as virulent as the Moloney strain. We propose a model for the emergence of the RadLV variants and show that the virus responsible for the high leukemogenic potential of BL/VL3 preparation is a nondefective, ecotropic, lymphotropic, nonfibrotropic, unique retrovirus which most likely arose from a parental primary RadLV similar to those studied here

  3. Multi-wavelength studies of TeV γ-ray emitting BL Lac objects

    Energy Technology Data Exchange (ETDEWEB)

    Kaufmann, Sarah Sabine

    2012-12-21

    The discovery of TeV γ-ray emission of BL Lac objects gave new insights in the particle acceleration and the emission processes of the highly relativistic jets. To shed light on the conditions in the high energetic jets of the TeV γ-ray emitting BL Lac objects, I have studied in great detail the spectral energy distribution (SED) of sources with different characteristics. BL Lac objects with exceptional very high energy spectra (soft and hard spectra) and with large differences in the emission peak frequencies, to cover the different classes of BL Lac objects, have been chosen. The basic aim of this thesis was, to study with new, simultaneous multi- avelength (MWL) observations, if the emission processes of these extreme cases of TeV BL Lac objects can be explained by the synchrotron Self-Compton (SSC) model which is well established for the class of BL Lac objects at lower energies. We proposed MWL observations in the optical, UV and X-ray regime, to be conducted simultaneous to very high energy observations with the H.E.S.S. experiment, to study the emission processes. Simultaneous observations are crucial, since BL Lac objects are variable at all wavebands. I have analysed the MWL observations and conducted detailed variability and spectral studies in each wavelength range. The different kind of absorption at each wavelength as well as the influence of the host galaxy of the AGN has been considered to obtain the intrinsic jet spectrum. I have then applied the commonly used theoretical jet model, the SSC model, to the SED. I conducted a MWL campaign on a BL Lac object with the softest TeV spectrum, PKS 2005-489, during which it was observed in a very bright X-ray state. The good spectral coverage of the emission peaks allowed a detailed study of the SSC model. The extreme BL Lac object 1ES 0229+200 exhibits a hard intrinsic TeV spectrum. With my MWL campaign I found a clear cut-off in the optical range and therefore a high minimum Lorentz factor is needed to

  4. Multi-wavelength studies of TeV γ-ray emitting BL Lac objects

    International Nuclear Information System (INIS)

    Kaufmann, Sarah Sabine

    2012-01-01

    The discovery of TeV γ-ray emission of BL Lac objects gave new insights in the particle acceleration and the emission processes of the highly relativistic jets. To shed light on the conditions in the high energetic jets of the TeV γ-ray emitting BL Lac objects, I have studied in great detail the spectral energy distribution (SED) of sources with different characteristics. BL Lac objects with exceptional very high energy spectra (soft and hard spectra) and with large differences in the emission peak frequencies, to cover the different classes of BL Lac objects, have been chosen. The basic aim of this thesis was, to study with new, simultaneous multi- avelength (MWL) observations, if the emission processes of these extreme cases of TeV BL Lac objects can be explained by the synchrotron Self-Compton (SSC) model which is well established for the class of BL Lac objects at lower energies. We proposed MWL observations in the optical, UV and X-ray regime, to be conducted simultaneous to very high energy observations with the H.E.S.S. experiment, to study the emission processes. Simultaneous observations are crucial, since BL Lac objects are variable at all wavebands. I have analysed the MWL observations and conducted detailed variability and spectral studies in each wavelength range. The different kind of absorption at each wavelength as well as the influence of the host galaxy of the AGN has been considered to obtain the intrinsic jet spectrum. I have then applied the commonly used theoretical jet model, the SSC model, to the SED. I conducted a MWL campaign on a BL Lac object with the softest TeV spectrum, PKS 2005-489, during which it was observed in a very bright X-ray state. The good spectral coverage of the emission peaks allowed a detailed study of the SSC model. The extreme BL Lac object 1ES 0229+200 exhibits a hard intrinsic TeV spectrum. With my MWL campaign I found a clear cut-off in the optical range and therefore a high minimum Lorentz factor is needed to

  5. File: International bilateral relations

    International Nuclear Information System (INIS)

    Feltin, Ch.; Rabouhams, J.; Bravo, X.; Rousseau, M.; Le Breton, S.; Saint Raymond, Ph.; Brigaud, O.; Pertuis, V.; McNair, J.; Sayers, M.R.; Bye, R.; Scherrer, J.

    1998-01-01

    Since its creation in 1973, the Authority of Safety was assigned missions in the international field with following objectives: to develop information exchanges with its foreign counterpart, to make know and to explain the French approach and practice; to give to concerned countries the useful information on french nuclear facilities situated near the border; This file shows with some examples, how bilateral relations allow to fill up these objectives and how the French Authority got the foreign experience. (N.C.)

  6. Dynamical Black Hole Masses of BL Lac Objects from the Sloan Digital Sky Survey

    NARCIS (Netherlands)

    Plotkin, Richard M.; Markoff, Sera; Trager, Scott C.; Anderson, Scott F.

    2012-01-01

    We measure black hole masses for 71 BL Lac objects from the Sloan Digital Sky Survey (SDSS) with redshifts out to z ∼ 0.4. We perform spectral decompositions of their nuclei from their host galaxies and measure their stellar velocity dispersions. Black hole masses are then derived from the black

  7. Absorption in the spectra of quasi stellar objects and BL Lac objects

    International Nuclear Information System (INIS)

    Perry, J.J.; Burbidge, E.M.; Burbidge, G.R.

    1978-06-01

    An extensive review is given of the observations of absorption in the spectra of QSOs and BL Lac objects. In Table Ia we summarize all of the information available up to May 1, 1978 on objects which show absorption. Following discussion of the observations in Section II, possible interpretations are critically discussed. (orig.) [de

  8. Regulation of methane oxidation in the facultative methanotroph Methylocella silvestris BL2.

    Science.gov (United States)

    Theisen, Andreas R; Ali, M Hanif; Radajewski, Stefan; Dumont, Marc G; Dunfield, Peter F; McDonald, Ian R; Dedysh, Svetlana N; Miguez, Carlos B; Murrell, J Colin

    2005-11-01

    The molecular regulation of methane oxidation in the first fully authenticated facultative methanotroph Methylocella silvestris BL2 was assessed during growth on methane and acetate. Problems of poor growth of Methylocella spp. in small-scale batch culture were overcome by growth in fermentor culture. The genes encoding soluble methane monooxygenase were cloned and sequenced, which revealed that the structural genes for soluble methane monooxygenase, mmoXYBZDC, were adjacent to two genes, mmoR and mmoG, encoding a sigma54 transcriptional activator and a putative GroEL-like chaperone, located downstream (3') of mmoC. Transcriptional analysis revealed that the genes were all cotranscribed from a sigma54-dependent promoter located upstream (5') of mmo X. The transcriptional start site was mapped. Transcriptional analysis of soluble methane monooxygenase genes and expression studies on fermentor grown cultures showed that acetate repressed transcription of sMMO in M. silvestris BL2. The possibility of the presence of a particulate, membrane-bound methane monooxygenase enzyme in M. silvestris BL2 and the copper-mediated regulation of soluble methane monooxygenase was investigated. Both were shown to be absent. A promoter probe vector was constructed and used to assay transcription of the promoter of the soluble methane monoxygenase genes of M. silvestris BL2 grown under various conditions and with different substrates. These data represent the first insights into the molecular physiology of a facultative methanotroph.

  9. Comparative physical mapping between wheat chromosome arm 2BL and rice chromosome 4.

    Science.gov (United States)

    Lee, Tong Geon; Lee, Yong Jin; Kim, Dae Yeon; Seo, Yong Weon

    2010-12-01

    Physical maps of chromosomes provide a framework for organizing and integrating diverse genetic information. DNA microarrays are a valuable technique for physical mapping and can also be used to facilitate the discovery of single feature polymorphisms (SFPs). Wheat chromosome arm 2BL was physically mapped using a Wheat Genome Array onto near-isogenic lines (NILs) with the aid of wheat-rice synteny and mapped wheat EST information. Using high variance probe set (HVP) analysis, 314 HVPs constituting genes present on 2BL were identified. The 314 HVPs were grouped into 3 categories: HVPs that match only rice chromosome 4 (298 HVPs), those that match only wheat ESTs mapped on 2BL (1), and those that match both rice chromosome 4 and wheat ESTs mapped on 2BL (15). All HVPs were converted into gene sets, which represented either unique rice gene models or mapped wheat ESTs that matched identified HVPs. Comparative physical maps were constructed for 16 wheat gene sets and 271 rice gene sets. Of the 271 rice gene sets, 257 were mapped to the 18-35 Mb regions on rice chromosome 4. Based on HVP analysis and sequence similarity between the gene models in the rice chromosomes and mapped wheat ESTs, the outermost rice gene model that limits the translocation breakpoint to orthologous regions was identified.

  10. Long-Term Optical Spectra Variability of BL Lacertae Object S5 ...

    Indian Academy of Sciences (India)

    —methods: periodogram analysis method, wavelet analysis method. 1. Introduction. S5 0716+714 is a typical BL Lac object, and it is very striking due to its observational properties. Heidt & Wagner (1996) found a period of 4 days through flux ...

  11. Photo-translocation of anti-HIV-1 drugs into TZM-bl cells

    CSIR Research Space (South Africa)

    Khanyile, T

    2013-04-01

    Full Text Available Targeted drug delivery into HIV-1 infected cells offers a reduction in toxicity and side effect. Using a femtosecond (fs) laser of different beam shapes anti-HIV-1 drugs are efficiently delivered into TZM-bl cells....

  12. Seven-Year Multi-Colour Optical Monitoring of BL Lacertae Object ...

    Indian Academy of Sciences (India)

    2016-01-27

    Jan 27, 2016 ... We monitored the BL Lac object S5 0716+714 in five intermediate optical passbands from 2004 September to 2011 April. The object was active most of the time and intra-day variability was frequently observed. The total variation amplitude tended to decrease with decreasing frequency. Strong ...

  13. F1 hybrids of BALB/c and C57BL/6 mouse strains respond differently ...

    Indian Academy of Sciences (India)

    cross was decided between C57BL/6 female and BALB/c ... on the mouse 7.4k cDNA microarray slide (University Health ... The ratio of two-fold increase (2.0) or decrease (−2.0) in ... were carried out to validate data from the cDNA micror- ray.

  14. Project Based Learning (PjBL) Practices at Politeknik Kota Bharu, Malaysia

    Science.gov (United States)

    Rahman, Md. Baharuddin Haji Abdul; Daud, Khairul Azhar Mat; Jusoff, Kamaruzaman; Ghani, Nik Azida Abd

    2009-01-01

    This study explores the utilization of Project-based Learning module in the subject of project development for the Mechanical Engineering students at Politeknik Kota Bharu. This study focuses on the development of the PjBL module based on socio-constructivist approach. The objective of this study is to explore the influence of the utilization of…

  15. Postnatal hematopoiesis and gut microbiota in NOD mice deviate from C57BL/6 mice

    DEFF Research Database (Denmark)

    Damlund, Dina Silke Malling; Metzdorff, Stine Broeng; Hasselby, Jane Preuss

    2016-01-01

    , a distinct splenic cell profile high in a granulocytic phenotype was evident in the neonatal NOD mice whereas neonatal C57BL/6 mice showed a profile rich in monocytes. Neonatal expression of Reg3g and Muc2 in the gut was deviating in NOD mice and coincided with fewer bacteria attaching to the Mucosal surface...

  16. Ethanol inhibits B16-BL6 melanoma metastasis and cell phenotypes associated with metastasis.

    Science.gov (United States)

    Kushiro, Kyoko; Núñez, Nomelí P

    2012-01-01

    Every year, approximately 68,000 new cases of malignant melanoma are diagnosed in the US. Ethanol consumption inhibits metastasis of melanoma in mice, but the mechanism is not well understood. C57BL/6J ob/+ mice, given either water or 20% ethanol, were injected intravenously with B16-BL6 melanoma cells to determine pulmonary metastasis. The effects of ethanol on cell phenotypes and markers of the epithelial-to-mesenchymal transition were determined in cell culture. In mice, ethanol consumption inhibited experimental pulmonary metastasis. This inhibition was associated with decreased body weight, and levels of systemic leptin, and insulin. In cell culture, ethanol inhibited B16-BL6 cell motility, invasion, and anchorage-independent growth. Additionally, ethanol reduced Snai1 expression and increased E-cadherin expression. Lastly, ethanol increased the expression of Kiss1 metastasis-suppressor and the metastasis suppressor Nm23/nucleoside diphosphate kinase. In both animal and in cell culture conditions, ethanol inhibited the metastatic ability of B16-BL6 melanoma cells.

  17. Computing Optical Variable Periods of BL Lac Object S5 0716+714 ...

    Indian Academy of Sciences (India)

    2016-01-27

    Jan 27, 2016 ... From a large volume of literature, we have collected effective observation of BL Lac object S5 0716+714 in the optical band, and constructed its long-term light curve from 1994 to 2006 AD. The light curve shows that S5 0716+714 is very active and exhibits very complicated non-sinusoidal variations.

  18. BL Lacertae: X-ray spectral evolution and a black-hole mass estimate

    Science.gov (United States)

    Titarchuk, Lev; Seifina, Elena

    2017-06-01

    We present an analysis of the spectral properties observed in X-rays from active galactic nucleus BL Lacertae using RXTE, Suzaku, ASCA, BeppoSAX, and Swift observations. The total time covered by these observations is approximately 20 yr. We show strong observational evidence that this source undergoes X-ray spectral transitions from the low hard state (LHS) through the intermediate state (IS) to the high soft state (HSS) during these observations. During the RXTE observations (1997-2001, 180 ks, for a total 145 datasets), the source was approximately 75%, 20% and only 5% of the time in the IS, LHS, and HSS, respectively. We also used Swift observations (470 datasets, for a total 800 ks), which occurred during 12 yr (2005-2016), the broadband (0.3-200 keV) data of BeppoSAX (1997-2000, 160 ks), and the low X-ray energy (0.3-10 keV) data of ASCA (1995-1999, 160 ks). Two observations of Suzaku (2006, 2013; 50 ks) in combinations with long-term RXTE and Swift data-sets fortunately allow us to describe all spectral states of BL Lac. The spectra of BL Lac are well fitted by the so-called bulk motion Comptonization (BMC) model for all spectral states. We have established the photon index saturation level, Γsat = 2.2 ± 0.1, in the Γ versus mass accretion rate (Ṁ) correlation. This Γ - Ṁ correlation allows us to estimate the black-hole (BH) mass in BL Lac to be MBH 3 × 107M⊙ for a distance of 300 Mpc. For the BH mass estimate, we use the scaling method taking stellar-mass Galactic BHs 4U 1543-47 and GX 339-4 as reference sources. The Γ - Ṁ correlation revealed in BL Lac is similar to those in a number of stellar-mass Galactic BHs and two recently studied intermediate-mass extragalactic BHs. It clearly shows the correlation along with the very extended Γ saturation at 2.2. This is robust observational evidence for the presence of a BH in BL Lac. We also reveal that the seed (disk) photon temperatures are relatively low, of order of 100 eV, which are consistent

  19. Stress and estrous cycle affect strategy but not performance of female C57BL/6J mice

    NARCIS (Netherlands)

    ter Horst, J.P.; Kentrop, J.; de Kloet, E.R.; Oitzl, M.S.

    2013-01-01

    Stress induces a switch in learning strategies of male C57BL/6J mice from predominantly spatial to more stimulus-response learning. To study generalization of these findings over sex, we investigated female C57BL/6J mice at three phases of the estrous cycle under non stress and acute (10 min)

  20. PFS: a distributed and customizable file system

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.

    1996-01-01

    In this paper we present our ongoing work on the Pegasus File System (PFS), a distributed and customizable file system that can be used for off-line file system experiments and on-line file system storage. PFS is best described as an object-oriented component library from which either a true file

  1. Huygens file service and storage architecture

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.; Stabell-Kulo, Tage; Stabell-Kulo, Tage

    1993-01-01

    The Huygens file server is a high-performance file server which is able to deliver multi-media data in a timely manner while also providing clients with ordinary “Unix” like file I/O. The file server integrates client machines, file servers and tertiary storage servers in the same storage

  2. Huygens File Service and Storage Architecture

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.; Stabell-Kulo, Tage; Stabell-Kulo, Tage

    1993-01-01

    The Huygens file server is a high-performance file server which is able to deliver multi-media data in a timely manner while also providing clients with ordinary “Unix” like file I/O. The file server integrates client machines, file servers and tertiary storage servers in the same storage

  3. 78 FR 75554 - Combined Notice of Filings

    Science.gov (United States)

    2013-12-12

    ...-000. Applicants: Young Gas Storage Company, Ltd. Description: Young Fuel Reimbursement Filing to be.... Protests may be considered, but intervention is necessary to become a party to the proceeding. eFiling is... qualifying facilities filings can be found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For...

  4. 12 CFR 5.4 - Filing required.

    Science.gov (United States)

    2010-01-01

    ... CORPORATE ACTIVITIES Rules of General Applicability § 5.4 Filing required. (a) Filing. A depository institution shall file an application or notice with the OCC to engage in corporate activities and... advise an applicant through a pre-filing communication to send the filing or submission directly to the...

  5. Junctional Adhesion Molecule (JAM)-C Deficient C57BL/6 Mice Develop a Severe Hydrocephalus

    Science.gov (United States)

    Liebner, Stefan; Mittelbronn, Michel; Deutsch, Urban; Enzmann, Gaby; Adams, Ralf H.; Aurrand-Lions, Michel; Plate, Karl H.; Imhof, Beat A.; Engelhardt, Britta

    2012-01-01

    The junctional adhesion molecule (JAM)-C is a widely expressed adhesion molecule regulating cell adhesion, cell polarity and inflammation. JAM-C expression and function in the central nervous system (CNS) has been poorly characterized to date. Here we show that JAM-C−/− mice backcrossed onto the C57BL/6 genetic background developed a severe hydrocephalus. An in depth immunohistochemical study revealed specific immunostaining for JAM-C in vascular endothelial cells in the CNS parenchyma, the meninges and in the choroid plexus of healthy C57BL/6 mice. Additional JAM-C immunostaining was detected on ependymal cells lining the ventricles and on choroid plexus epithelial cells. Despite the presence of hemorrhages in the brains of JAM-C−/− mice, our study demonstrates that development of the hydrocephalus was not due to a vascular function of JAM-C as endothelial re-expression of JAM-C failed to rescue the hydrocephalus phenotype of JAM-C−/− C57BL/6 mice. Evaluation of cerebrospinal fluid (CSF) circulation within the ventricular system of JAM-C−/− mice excluded occlusion of the cerebral aqueduct as the cause of hydrocephalus development but showed the acquisition of a block or reduction of CSF drainage from the lateral to the 3rd ventricle in JAM-C−/− C57BL/6 mice. Taken together, our study suggests that JAM-C−/− C57BL/6 mice model the important role for JAM-C in brain development and CSF homeostasis as recently observed in humans with a loss-of-function mutation in JAM-C. PMID:23029139

  6. The Galley Parallel File System

    Science.gov (United States)

    Nieuwejaar, Nils; Kotz, David

    1996-01-01

    Most current multiprocessor file systems are designed to use multiple disks in parallel, using the high aggregate bandwidth to meet the growing I/0 requirements of parallel scientific applications. Many multiprocessor file systems provide applications with a conventional Unix-like interface, allowing the application to access multiple disks transparently. This interface conceals the parallelism within the file system, increasing the ease of programmability, but making it difficult or impossible for sophisticated programmers and libraries to use knowledge about their I/O needs to exploit that parallelism. In addition to providing an insufficient interface, most current multiprocessor file systems are optimized for a different workload than they are being asked to support. We introduce Galley, a new parallel file system that is intended to efficiently support realistic scientific multiprocessor workloads. We discuss Galley's file structure and application interface, as well as the performance advantages offered by that interface.

  7. 75 FR 51994 - Combined Notice of Filings

    Science.gov (United States)

    2010-08-24

    ...: Panther Interstate Pipeline Energy, LLC. Description: Panther Interstate Pipeline Energy, LLC submits tariff filing per 154.203: Panther Baseline eTariff Filing to be effective 8/ 12/2010. Filed Date: 08/13...

  8. 76 FR 52323 - Combined Notice of Filings; Filings Instituting Proceedings

    Science.gov (United States)

    2011-08-22

    .... Applicants: Young Gas Storage Company, Ltd. Description: Young Gas Storage Company, Ltd. submits tariff..., but intervention is necessary to become a party to the proceeding. The filings are accessible in the.... More detailed information relating to filing requirements, interventions, protests, and service can be...

  9. StagBL : A Scalable, Portable, High-Performance Discretization and Solver Layer for Geodynamic Simulation

    Science.gov (United States)

    Sanan, P.; Tackley, P. J.; Gerya, T.; Kaus, B. J. P.; May, D.

    2017-12-01

    StagBL is an open-source parallel solver and discretization library for geodynamic simulation,encapsulating and optimizing operations essential to staggered-grid finite volume Stokes flow solvers.It provides a parallel staggered-grid abstraction with a high-level interface in C and Fortran.On top of this abstraction, tools are available to define boundary conditions and interact with particle systems.Tools and examples to efficiently solve Stokes systems defined on the grid are provided in small (direct solver), medium (simple preconditioners), and large (block factorization and multigrid) model regimes.By working directly with leading application codes (StagYY, I3ELVIS, and LaMEM) and providing an API and examples to integrate with others, StagBL aims to become a community tool supplying scalable, portable, reproducible performance toward novel science in regional- and planet-scale geodynamics and planetary science.By implementing kernels used by many research groups beneath a uniform abstraction layer, the library will enable optimization for modern hardware, thus reducing community barriers to large- or extreme-scale parallel simulation on modern architectures. In particular, the library will include CPU-, Manycore-, and GPU-optimized variants of matrix-free operators and multigrid components.The common layer provides a framework upon which to introduce innovative new tools.StagBL will leverage p4est to provide distributed adaptive meshes, and incorporate a multigrid convergence analysis tool.These options, in addition to a wealth of solver options provided by an interface to PETSc, will make the most modern solution techniques available from a common interface. StagBL in turn provides a PETSc interface, DMStag, to its central staggered grid abstraction.We present public version 0.5 of StagBL, including preliminary integration with application codes and demonstrations with its own demonstration application, StagBLDemo. Central to StagBL is the notion of an

  10. The DNA Files

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-06-09

    The DNA Files is a radio documentary which disseminates genetics information over public radio. The documentaries explore subjects which include the following: How genetics affects society. How human life began and how it evolved. Could new prenatal genetic tests hold the key to disease prevention later in life? Would a national genetic data base sacrifice individual privacy? and Should genes that may lead to the cure for cancer be privately owned? This report serves as a project update for the second quarter of 1998. It includes the spring/summer 1998 newsletter, the winter 1998 newsletter, the program clock, and the latest flyer.

  11. Earnings Public-Use File, 2006

    Data.gov (United States)

    Social Security Administration — Social Security Administration released Earnings Public-Use File (EPUF) for 2006. File contains earnings information for individuals drawn from a systematic random...

  12. Portable File Format (PFF) specifications

    Energy Technology Data Exchange (ETDEWEB)

    Dolan, Daniel H. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-02-01

    Created at Sandia National Laboratories, the Portable File Format (PFF) allows binary data transfer across computer platforms. Although this capability is supported by many other formats, PFF files are still in use at Sandia, particularly in pulsed power research. This report provides detailed PFF specifications for accessing data without relying on legacy code.

  13. Software for Managing Personal Files.

    Science.gov (United States)

    Lundeen, Gerald

    1989-01-01

    Discusses the special characteristics of personal file management software and compares four microcomputer software packages: Notebook II with Bibliography and Convert, Pro-Cite with Biblio-Links, askSam, and Reference Manager. Each package is evaluated in terms of the user interface, file maintenance, retrieval capabilities, output, and…

  14. Mixed-Media File Systems

    NARCIS (Netherlands)

    Bosch, H.G.P.

    1999-01-01

    This thesis addresses the problem of implementing mixed-media storage systems. In this work a mixed-media file system is defined to be a system that stores both conventional (best-effort) file data and real-time continuous-media data. Continuous-media data is usually bulky, and servers storing and

  15. stní Agenda 21

    OpenAIRE

    CENIA, česká informační agentura životního prostředí

    2013-01-01

    Veletrh Česká příroda, kterým Ministerstvo životního prostředí současně zahájilo celoroční kampaň se stejným názvem, se uskutečnil v pátek 19. a sobotu 20. dubna 2013 od 10 do 18 hodin v prostorách pražského Národního domu na Vinohradech. Veletrh podpořil svou návštěvou v sobotu 20. dubna 2013 ministr životního prostředí Tomáš Chalupa, který zároveň pokřtil knihu Nejkrásnější výlety do chráněných krajinných oblastí ČR. Bohatý doprovodný program probíhal souběžně na několika místech Národního ...

  16. Storing files in a parallel computing system using list-based index to identify replica files

    Science.gov (United States)

    Faibish, Sorin; Bent, John M.; Tzelnic, Percy; Zhang, Zhenhua; Grider, Gary

    2015-07-21

    Improved techniques are provided for storing files in a parallel computing system using a list-based index to identify file replicas. A file and at least one replica of the file are stored in one or more storage nodes of the parallel computing system. An index for the file comprises at least one list comprising a pointer to a storage location of the file and a storage location of the at least one replica of the file. The file comprises one or more of a complete file and one or more sub-files. The index may also comprise a checksum value for one or more of the file and the replica(s) of the file. The checksum value can be evaluated to validate the file and/or the file replica(s). A query can be processed using the list.

  17. Design and creation of a direct access nuclear data file

    International Nuclear Information System (INIS)

    Charpentier, P.

    1981-06-01

    General considerations on the structure of instructions and files are reviewed. Design, organization and mode of use of the different files: instruction file, index files, inverted files, automatic analysis and inquiry programs are examined [fr

  18. X-ray emission from BL Lac objects: Comparison to the synchrotron self-Compton models

    International Nuclear Information System (INIS)

    Schwartz, D.A.; Madejski, G.; Ku, W.H.-M.

    1982-01-01

    As one part of our joint study of the X-ray properties of BL Lac objects, the authors compare the measured X-ray flux densities with those predicted using the synchrotron self-Compton (SSC) formalism (Jones et al. 1974). Naive application of the formalism predicts X-ray fluxes from 10 -3 to 10 5 those observed. They therefore ask what we can learn by simply assuming the SSC mechanism, and looking for ways to reconcile the observed and measured X-ray fluxes. This paper reports investigation of beaming factors due to relativistic ejection of a radiation source which is isotropic in its own rest frame. The authors conclude that large Lorentz factors, GAMMA approximately > 10, do not apply to BL Lac objects as a class. (Auth.)

  19. Three new BL Lacertae objects in the Palomar-Green survey

    Science.gov (United States)

    Fleming, Thomas A.; Green, Richard F.; Jannuzi, Buell T.; Liebert, James; Smith, Paul S.; Fink, Henner

    1993-01-01

    We have identified three BL Lacertae objects in the Palomar-Green Survey which were previously misclassified as DC white dwarfs, namely PG 1246+586, PG 1424+240, and PG 1437+398. Our reclassification is based on the detection of these objects as x-ray sources in the ROSAT all-sky survey and upon our subsequent detection of intrinsic linearly polarized and variable optical emission from these sources. As a result of the ROSAT survey, the number of identified BL Lac objects in the Palomar-Green catalog of UV excess objects has been doubled. Corrected optical positions are presented for PG 1246+586 and PG 1437+398.

  20. The ultraviolet to X-ray continua of BL Lac objects

    International Nuclear Information System (INIS)

    George, I.M.; Warwick, R.S.; McHardy, I.M.

    1988-01-01

    The results from EXOSAT observations of three X-ray bright BL Lacertae objects, Mrk 501, 1218 + 304 and Mrk 180 are presented. All three sources have soft power-law X-ray spectra with low-energy cut-offs consistent with absorption in the line-of-sight gas column density through our own galaxy. The three objects also exhibit significant spectral variability in the X-ray band on time-scales ranging from a few days to a year. In each case, X-ray flux and spectral index appear to be correlated, in the sense that the X-ray spectrum hardens as the source brightens. The intrinsic ultraviolet to X-ray spectrum of these and several other X-ray bright BL Lac objects can be modelled as a power-law continuum of energy index ∼1.0 below about 0.1 keV, above which the spectral slope steepens. (author)

  1. CNN-BLPred: a Convolutional neural network based predictor for β-Lactamases (BL) and their classes.

    Science.gov (United States)

    White, Clarence; Ismail, Hamid D; Saigo, Hiroto; Kc, Dukka B

    2017-12-28

    The β-Lactamase (BL) enzyme family is an important class of enzymes that plays a key role in bacterial resistance to antibiotics. As the newly identified number of BL enzymes is increasing daily, it is imperative to develop a computational tool to classify the newly identified BL enzymes into one of its classes. There are two types of classification of BL enzymes: Molecular Classification and Functional Classification. Existing computational methods only address Molecular Classification and the performance of these existing methods is unsatisfactory. We addressed the unsatisfactory performance of the existing methods by implementing a Deep Learning approach called Convolutional Neural Network (CNN). We developed CNN-BLPred, an approach for the classification of BL proteins. The CNN-BLPred uses Gradient Boosted Feature Selection (GBFS) in order to select the ideal feature set for each BL classification. Based on the rigorous benchmarking of CCN-BLPred using both leave-one-out cross-validation and independent test sets, CCN-BLPred performed better than the other existing algorithms. Compared with other architectures of CNN, Recurrent Neural Network, and Random Forest, the simple CNN architecture with only one convolutional layer performs the best. After feature extraction, we were able to remove ~95% of the 10,912 features using Gradient Boosted Trees. During 10-fold cross validation, we increased the accuracy of the classic BL predictions by 7%. We also increased the accuracy of Class A, Class B, Class C, and Class D performance by an average of 25.64%. The independent test results followed a similar trend. We implemented a deep learning algorithm known as Convolutional Neural Network (CNN) to develop a classifier for BL classification. Combined with feature selection on an exhaustive feature set and using balancing method such as Random Oversampling (ROS), Random Undersampling (RUS) and Synthetic Minority Oversampling Technique (SMOTE), CNN-BLPred performs

  2. Sub-Hour X-Ray Variability of High-Energy Peaked BL Lacertae Objects

    OpenAIRE

    Bidzina Kapanadze

    2018-01-01

    The study of multi-wavelength flux variability in BL Lacertae objects is very important to discern unstable processes and emission mechanisms underlying their extreme observational features. While the innermost regions of these objects are not accessible from direct observations, we may draw conclusions about their internal structure via the detection of flux variations on various timescales, based on the light-travel argument. In this paper, we review the sub-hour X-ray variability in high-e...

  3. Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2.

    Science.gov (United States)

    Chen, Yin; Crombie, Andrew; Rahman, M Tanvir; Dedysh, Svetlana N; Liesack, Werner; Stott, Matthew B; Alam, Maqsudul; Theisen, Andreas R; Murrell, J Colin; Dunfield, Peter F

    2010-07-01

    Methylocella silvestris BL2 is an aerobic methanotroph originally isolated from an acidic forest soil in Germany. It is the first fully authenticated facultative methanotroph. It grows not only on methane and other one-carbon (C(1)) substrates, but also on some compounds containing carbon-carbon bonds, such as acetate, pyruvate, propane, and succinate. Here we report the full genome sequence of this bacterium.

  4. Complete Genome Sequence of the Aerobic Facultative Methanotroph Methylocella silvestris BL2▿

    Science.gov (United States)

    Chen, Yin; Crombie, Andrew; Rahman, M. Tanvir; Dedysh, Svetlana N.; Liesack, Werner; Stott, Matthew B.; Alam, Maqsudul; Theisen, Andreas R.; Murrell, J. Colin; Dunfield, Peter F.

    2010-01-01

    Methylocella silvestris BL2 is an aerobic methanotroph originally isolated from an acidic forest soil in Germany. It is the first fully authenticated facultative methanotroph. It grows not only on methane and other one-carbon (C1) substrates, but also on some compounds containing carbon-carbon bonds, such as acetate, pyruvate, propane, and succinate. Here we report the full genome sequence of this bacterium. PMID:20472789

  5. Complete Genome Sequence of the Aerobic Facultative Methanotroph Methylocella silvestris BL2▿

    OpenAIRE

    Chen, Yin; Crombie, Andrew; Rahman, M. Tanvir; Dedysh, Svetlana N.; Liesack, Werner; Stott, Matthew B.; Alam, Maqsudul; Theisen, Andreas R.; Murrell, J. Colin; Dunfield, Peter F.

    2010-01-01

    Methylocella silvestris BL2 is an aerobic methanotroph originally isolated from an acidic forest soil in Germany. It is the first fully authenticated facultative methanotroph. It grows not only on methane and other one-carbon (C1) substrates, but also on some compounds containing carbon-carbon bonds, such as acetate, pyruvate, propane, and succinate. Here we report the full genome sequence of this bacterium.

  6. The influences of age on T lymphocyte subsets in C57BL/6 mice

    Directory of Open Access Journals (Sweden)

    Jing Xie

    2017-01-01

    Full Text Available The aim of this study is to evaluate the age related changes of T lymphocyte subsets in C57BL/6 mice and immune function. Multi-color immunofluorescence techniques that were used to analyse relative numbers of T lymphocyte subsets include CD4+, CD8+, naive and memory CD4+ and CD8+, CD8+CD28+ T cells in peripheral blood of C57BL/6 mice from different age groups (Group I: 2 months old; Group II: 7 months old; Group III: 21 months old; Splenocytes isolated from different group mice were stimulated with Con A to evaluate the proliferative ability. Compared with group I, group II had a significant reduction in the percentage of CD4+, naive CD4+ and CD8+ T cells and an increase in the percentage of CD8+ T cells, while group III had a significant reduction in the percentage of CD4+, naive CD4+ and CD8+ T cells and increase in the percentage of CD8+, memory CD4+ and CD8+ T cells in peripheral blood. Compared with group II, group III had a significant reduction in the percentage of naive CD8+ T cells and increase in the percentage of memory CD4+ and CD8+, CD8+CD28+ T cells in peripheral blood. The T lymphocyte proliferation in vitro showed that groups II and III had a lower proliferative capacity than group I, between groups II and III, there was not a significant difference. We provide relative values for the T lymphocyte subsets in the different age groups of C57BL/6 mice. The immune system began aging at 7 months old in C57BL/6 mice under a specific pathogen free environment.

  7. Correlated radio and optical variability in the BL Lacertae object 0716 + 714

    International Nuclear Information System (INIS)

    Quirrenbach, A.; Witzel, A.; Krichbaum, T.P.; Wagner, S.; Sanchez-pons, F.

    1991-01-01

    Results are presented from simultaneous optical and radio observations of the BL Lacertae object 0716 + 714. During a 4-week period of continuous monitoring the source displayed in both wavelength regimes a transition between states of fast and slow variability with a change of the typical variability time scale from about 1 day to about 7 days. The simultaneous transition is interpreted as evidence for intrinsic source variability, and some consequences for the optical and radio emission regions are discussed. 19 refs

  8. The experimental apparatus for synchrotron radiation Moessbauer spectroscopy of BL11 in SPring-8

    International Nuclear Information System (INIS)

    Mitsui, T.; Kitao, S.; Zhang, X.W.; Marushita, M.; Seto, M.

    2001-01-01

    Synchrotron radiation Moessbauer spectroscopy (time spectrum of nuclear forward scattering and nuclear resonant inelastic scattering) enables us to study both the electronic state and lattice dynamics of a target material. Furthermore, the excellent properties of synchrotron radiation (polarization, pulse, small beam size) promise us the unique studies for material science. In order to progress in these studies, some experimental apparatuses were installed in BL11XU of SPring-8

  9. Identification of Salt-Tolerant Sinorhizobium sp Strain BL3 Membrane Proteins Based on Proteomics

    DEFF Research Database (Denmark)

    Tanthanuch, Waraporn; Mohammed, Shabaz; Matthiesen, Rune

    2010-01-01

    functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line...... SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress....

  10. Temporal characteristics of stress-induced decrease in benzodiazepine reception in C57BL/6 and BALB/c mice.

    Science.gov (United States)

    Yarkova, M A; Seredenin, S B

    2014-10-01

    We studied the duration of the drop of specific (3)H-flunitrazepam binding by synaptosomal membranes from the brain of C57Bl/6 and BALB/c mice after open-field and "contact with predator" tests. It was found that reduced benzodiazepine reception in BALB/c mice after open-field test persisted for 1.5 h, but no changes of this parameter were found in C57Bl/6 mice. After contact with predator, the binding capacity of the benzodiazepine site of GABAA receptor was reduced for 8 h in BALB/c mice and for 24 h in C57Bl/6 mice.

  11. USING THE METHODS OF WAVELET ANALYSIS AND SINGULAR SPECTRUM ANALYSIS IN THE STUDY OF RADIO SOURCE BL LAC

    OpenAIRE

    Donskykh, G. I.; Ryabov, M. I.; Sukharev, A. I.; Aller, M.

    2014-01-01

    We investigated the monitoring data of extragalactic source BL Lac. This monitoring was held withUniversityofMichigan26-meter radio  telescope. To study flux density of extragalactic source BL Lac at frequencies of 14.5, 8 and 4.8 GHz, the wavelet analysis and singular spectrum analysis were used. Calculating the integral wavelet spectra allowed revealing long-term  components  (~7-8 years) and short-term components (~ 1-4 years) in BL Lac. Studying of VLBI radio maps (by the program Mojave) ...

  12. Macrophage-dependent clearance of systemically administered B16BL6-derived exosomes from the blood circulation in mice

    OpenAIRE

    Imai, Takafumi; Takahashi, Yuki; Nishikawa, Makiya; Kato, Kana; Morishita, Masaki; Yamashita, Takuma; Matsumoto, Akihiro; Charoenviriyakul, Chonlada; Takakura, Yoshinobu

    2015-01-01

    Previous studies using B16BL6-derived exosomes labelled with gLuc–lactadherin (gLuc-LA), a fusion protein of Gaussia luciferase (a reporter protein) and lactadherin (an exosome-tropic protein), showed that the exosomes quickly disappeared from the systemic circulation after intravenous injection in mice. In the present study, the mechanism of rapid clearance of intravenously injected B16BL6 exosomes was investigated. gLuc-LA-labelled exosomes were obtained from supernatant of B16BL6 cells aft...

  13. Neutrino mass, leptogenesis and FIMP dark matter in a U(1){sub B-L} model

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, Anirban; Khan, Sarif [Harish-Chandra Research Institute, Allahabad (India); Homi Bhabha National Institute, Training School Complex, Mumbai (India); Choubey, Sandhya [Harish-Chandra Research Institute, Allahabad (India); Homi Bhabha National Institute, Training School Complex, Mumbai (India); AlbaNova University Center, Department of Theoretical Physics, School of Engineering Sciences, KTH Royal Institute of Technology, Stockholm (Sweden)

    2017-12-15

    The Standard Model (SM) is inadequate to explain the origin of tiny neutrino masses, the dark matter (DM) relic abundance and the baryon asymmetry of the Universe. In this work, to address all three puzzles, we extend the SM by a local U(1){sub B-L} gauge symmetry, three right-handed (RH) neutrinos for the cancellation of gauge anomalies and two complex scalars having non-zero U(1){sub B-L} charges. All the newly added particles become massive after the breaking of the U(1){sub B-L} symmetry by the vacuum expectation value (VEV) of one of the scalar fields φ{sub H}. The other scalar field, φ{sub DM}, which does not have any VEV, becomes automatically stable and can be a viable DM candidate. Neutrino masses are generated using the Type-I seesaw mechanism, while the required lepton asymmetry to reproduce the observed baryon asymmetry can be attained from the CP violating out of equilibrium decays of the RH neutrinos in TeV scale. More importantly within this framework, we study in detail the production of DM via the freeze-in mechanism considering all possible annihilation and decay processes. Finally, we find a situation when DM is dominantly produced from the annihilation of the RH neutrinos, which are at the same time also responsible for neutrino mass generation and leptogenesis. (orig.)

  14. Neutrino mass, leptogenesis and FIMP dark matter in a U(1)_{B-L} model

    Science.gov (United States)

    Biswas, Anirban; Choubey, Sandhya; Khan, Sarif

    2017-12-01

    The Standard Model (SM) is inadequate to explain the origin of tiny neutrino masses, the dark matter (DM) relic abundance and the baryon asymmetry of the Universe. In this work, to address all three puzzles, we extend the SM by a local U(1)_{B-L} gauge symmetry, three right-handed (RH) neutrinos for the cancellation of gauge anomalies and two complex scalars having non-zero U(1)_{B-L} charges. All the newly added particles become massive after the breaking of the U(1)_{B-L} symmetry by the vacuum expectation value (VEV) of one of the scalar fields φ _H. The other scalar field, φ _DM, which does not have any VEV, becomes automatically stable and can be a viable DM candidate. Neutrino masses are generated using the Type-I seesaw mechanism, while the required lepton asymmetry to reproduce the observed baryon asymmetry can be attained from the CP violating out of equilibrium decays of the RH neutrinos in TeV scale. More importantly within this framework, we study in detail the production of DM via the freeze-in mechanism considering all possible annihilation and decay processes. Finally, we find a situation when DM is dominantly produced from the annihilation of the RH neutrinos, which are at the same time also responsible for neutrino mass generation and leptogenesis.

  15. The REX survey as a tool to test the beaming model for BL Lacs

    CERN Document Server

    Caccianiga, A; MacCacaro, T; Wolter, A; Gioia, I M

    1999-01-01

    In the context of the beaming model (BM) BL Lac and FR I radio galaxies are thought to be the same class of sources seen at different angles. If this picture is correct, we expect to find some transition objects with intermediate properties between the two classes. To date, this intermediate population of objects is missing, probably due to the limiting fluxes of the current X-ray/radio surveys and/or to the criteria used to separate BL Lacs from normal elliptical galaxies. As pointed out by Browne and Marcha (1993), the detection of the transition objects requires particular attention since the "weak" BL Lac nucleus is hidden by the light of the host elliptical galaxy. A useful criterion often used to assess the presence of a non-thermal source, in addition to the stellar emission of the host galaxy, is the Ca contrast at 4000 AA (K/sub 4000/). This quantity, which is defined as the relative depression of the spectrum across 4000 AA, is a typical feature observed in a "normal" elliptical galaxy; the samples ...

  16. EXPERIMENTAL SUBCUTANEOUS CYSTICERCOSIS BY Taenia crassiceps IN BALB/c AND C57BL/6 MICE.

    Science.gov (United States)

    Pereira, Íria Márcia; Lima, Sarah Buzaim; Freitas, Aline de Araújo; Vinaud, Marina Clare; Junior, Ruy de Souza Lino

    2016-07-11

    Human cysticercosis is one of the most severe parasitic infections affecting tissues. Experimental models are needed to understand the host-parasite dynamics involved throughout the course of the infection. The subcutaneous experimental model is the closest to what is observed in human cysticercosis that does not affect the central nervous system. The aim of this study was to evaluate macroscopically and microscopically the experimental subcutaneous cysticercosis caused by Taenia crassiceps cysticerci in BALB/c and C57BL/6 mice. Animals were inoculated in the dorsal subcutaneous region and macroscopic and microscopic aspects of the inflammatory process in the host-parasite interface were evaluated until 90 days after the inoculation (DAI). All the infected animals presented vesicles containing cysticerci in the inoculation site, which was translucent at 7 DAI and then remained opaque throughout the experimental days. The microscopic analysis showed granulation tissue in BALB/c mice since the acute phase of infection evolving to chronicity without cure, presenting 80% of larval stage cysticerci at 90 DAI. While C57BL/6 mice presented 67% of final stage cysticerci at 90 DAI, the parasites were surrounded by neutrophils evolving to the infection control. It is possible to conclude that the genetic features of susceptibility (BALB/c) or resistance (C57BL/6) were confirmed in an experimental subcutaneous model of cysticercosis.

  17. Type II Cepheids: evidence for Na-O anticorrelation for BL Her type stars?

    Science.gov (United States)

    Kovtyukh, V.; Yegorova, I.; Andrievsky, S.; Korotin, S.; Saviane, I.; Lemasle, B.; Chekhonadskikh, F.; Belik, S.

    2018-06-01

    The chemical composition of 28 Population II Cepheids and one RR Lyrae variable has been studied using high-resolution spectra. The chemical composition of W Vir variable stars (with periods longer than 8 d) is typical for the halo and thick disc stars. However, the chemical composition of BL Her variables (with periods of 0.8-4 d) is drastically different, although it does not differ essentially from that of the stars belonging to globular clusters. In particular, the sodium overabundance ([Na/Fe] ≈ 0.4) is reported for most of these stars, and the Na-O anticorrelation is also possible. The evolutionary tracks for BL Her variables (with a progenitor mass value of 0.8 solar masses) indicate that mostly helium-overabundant stars (Y = 0.30-0.35) can fall into the instability strip region. We suppose that it is the helium overabundance that accounts not only for the existence of BL Her variable stars but also for the observed abnormalities in the chemical composition of this small group of pulsating variables.

  18. Quantitative SRXRF analysis on the BL15U1 beamline at SSRF

    International Nuclear Information System (INIS)

    Zhang Yanle; Yu Xiaohan

    2010-01-01

    In this paper, we give an introduction first to two quantification methods for synchrotron radiation X-ray fluorescence analysis (SRXRF), namely fundamental parameters method and Monte-Carlo simulation method, for their application on the BL15U1 beamline (hard X-ray microprobe) at SSRF (Shanghai Synchrotron Radiation Facility). Effectiveness of the two methods is demonstrated and the XRF detection limits of the BL15U1 beamline are calculated. The results show that, quantitative analysis at the ppm level can be done using the two methods, with an accuracy of better than 10%. Although both the methods are valid for the SRXRF data analysis,the Monte Carlo method gives better analysis result, as it compares the simulated spectrum with the experiment spectrum, and this helps the determination of experiment parameters and thus minimizes the error caused by incorrect parameters. Finally, the detection limits shows that the BL15U1 beamline is capable of carrying out standard-of-the-art XRF experiment. (authors)

  19. Total alkaloid content in various fractions of Tabernaemonata sphaerocarpa Bl. (Jembirit) leaves

    Science.gov (United States)

    Salamah, N.; Ningsih, D. S.

    2017-11-01

    Tabernaemontana sphaerocarpa Bl. (Jembirit) is one of the Apocynaceae family plants containing alkaloid compound. Traditionally, it is used as an anti-inflammatory medicine. It is found to have a new bisindole alkaloid compound that shows a potent cytotoxic activity in human cancer. This study aimed to know the total alkaloid content in some fractions of ethanolic extract of T. sphaerocarpa Bl. leaf powder was extracted by maceration method in 70% ethanol solvent. Then, the extract was fractionated in a separatory funnel using water, ethyl acetate, and hexane. The total alkaloid content in each fraction was analyzed with visible spectrophotometric methods based on the reaction with Bromocresol Green (BCG). The total alkaloids in water fraction and ethyl acetate fraction were (0.0312±0.0009)% and (0.0281±0.0014)%, respectively. Meanwhile, the total alkaloid content in hexane was not detected. The statistical analysis, performed in SPSS, resulted in a significant difference between the total alkaloids in water fraction and ethyl acetate fraction. The total alkaloid in water fraction of T. sphaerocarpa Bl. was higher than the one in ethyl acetate fraction.

  20. NEW HIGH- z FERMI BL LACS WITH THE PHOTOMETRIC DROPOUT TECHNIQUE

    Energy Technology Data Exchange (ETDEWEB)

    Kaur, A.; Ajello, M.; Hartmann, D. H.; Paliya, V. S. [Department of Physics and Astronomy, Clemson University, SC 29634-0978 (United States); Rau, A.; Greiner, J.; Bolmer, J.; Schady, P. [Max-Planck-Institut für extraterrestrische Physik, Giessenbachstraße 1, D-85748 Garching (Germany); Domínguez, A., E-mail: akaur@g.clemson.edu [Grupo de Altas Energías, Universidad Complutense, E-28040 Madrid (Spain)

    2017-01-01

    Determining redshifts for BL Lacertae (BL Lac) objects using the traditional spectroscopic method is challenging due to the absence of strong emission lines in their optical spectra. We employ the photometric dropout technique to determine redshifts for this class of blazars using the combined 13 broadband filters from Swift -UVOT and the multi-channel imager GROND at the MPG 2.2 m telescope at ESO's La Silla Observatory. The wavelength range covered by these 13 filters extends from far-ultraviolet to the near-infrared. We report results on 40 new Fermi- detected BL Lacs with the photometric redshift determinations for five sources, with 3FGL J1918.2–4110 being the most distant in our sample at z  = 2.16. Reliable upper limits are provided for 20 sources in this sample. Using the highest energy photons for these Fermi -LAT sources, we evaluate the consistency with the gamma-ray horizon due to the extragalactic background light.

  1. Screening of sugarcane somaclones of variety bl4 for agronomic characteristics

    International Nuclear Information System (INIS)

    Raza, S.; Qamarunnisa, S.; Jamil, I.

    2014-01-01

    Considering the environmental conditions of Pakistan where sugarcane breeding is constrained due to non viable fuzz (seeds) production. Somaclonal variation could prove to be a useful tool to overcome the difficulties in cane breeding. In the present study, 324 sugarcane somaclones regenerated from immature leaf roll callus of sugarcane variety BL4 were evaluated for their yield and yield contributing characters and the quality traits of cane. The field trial of somaclones showed variation in 160 somaclones from the mother plant in at least one character observed. Most of the somaclones showed variation in weight of stalks per plant; however, only twenty four out of 89 clones showed increase in the weight of the stalks per clump. The second highly variable trait was the number of stalks, 88 plants showed either increase or decrease in the number of stalks. It is noteworthy that the sucrose accumulation was not increased in any of the somaclones. Twenty one somaclones were selected for their increased yield potential. The comparative performance of these selected clones revealed that clones 'K-250, K-265, K-251, K-109, K-106, K-300 and K-315 gave better sugar yield /plant as compared to BL4. Maximum sugar yield/plant was observed in Clone 'K-250' (2.5 Kg) followed by K-265 (2.44 Kg), whereas the average sugar yield of BL4 was 1.2 Kg/plant. (author)

  2. Lycopene pretreatment improves hepatotoxicity induced by acetaminophen in C57BL/6 mice.

    Science.gov (United States)

    Bandeira, Ana Carla Balthar; da Silva, Rafaella Cecília; Rossoni, Joamyr Victor; Figueiredo, Vivian Paulino; Talvani, André; Cangussú, Silvia Dantas; Bezerra, Frank Silva; Costa, Daniela Caldeira

    2017-02-01

    Acetaminophen (APAP) is an antipyretic and analgesic drug that, in high doses, leads to severe liver injury and potentially death. Oxidative stress is an important event in APAP overdose. Researchers are looking for natural antioxidants with the potential to mitigate the harmful effects of reactive oxygen species in different models. Lycopene has been widely studied for its antioxidant properties. The aim of this study was to evaluate the antioxidant potential of lycopene pretreatment in APAP-induced liver injury in C57BL/6 mice. C57BL/6 male mice were divided into the following groups: control (C); sunflower oil (CO); acetaminophen 500mg/kg (APAP); acetaminophen 500mg/kg+lycopene 10mg/kg (APAP+L10), and acetaminophen 500mg/kg+lycopene 100mg/kg (APAP+L100). Mice were pretreated with lycopene for 14 consecutive days prior to APAP overdose. Analyses of blood serum and livers were performed. Lycopene was able to improve redox imbalance, decrease thiobarbituric acid reactive species level, and increase CAT and GSH levels. In addition, it decreased the IL-1β expression and the activity of MMP-2. This study revealed that preventive lycopene consumption in C57BL/6 mice can attenuate the effects of APAP-induced liver injury. Furthermore, by improving the redox state, and thus indicating its potential antioxidant effect, lycopene was also shown to have an influence on inflammatory events. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Immunobiologic effects of cytokine gene transfer of the B16-BL6 melanoma.

    Science.gov (United States)

    Strome, S E; Krauss, J C; Cameron, M J; Forslund, K; Shu, S; Chang, A E

    1993-12-01

    The genetic modification of tumors offers an approach to modulate the host immune response to relatively weak native tumor antigens. We examined the immunobiologic effects of various cytokine genes transferred into the poorly immunogenic B16-BL6 murine melanoma. Retroviral expression vectors containing cDNAs for interleukin 2, interleukin 4, interferon gamma, or a neomycin-resistant control were electroporated into a B16-BL6 tumor clone. Selected transfected clones were examined for in vitro cytokine secretion and in vivo tumorigenicity. When cells from individual clones were injected intradermally into syngeneic mice, the interleukin 4-secreting clone grew significantly slower than did the neomycin-resistant transfected control, while the growth of the interleukin 2- and interferon gamma-expressing clones was not affected. Despite minimal cytokine secretion by interferon gamma-transfected cells, these cells expressed upregulated major histocompatibility class I antigen and were more susceptible to lysis by allosensitized cytotoxic T lymphocytes compared with parental or neomycin-resistant transfected tumor targets. We observed diverse immunobiologic effects associated with cytokine gene transfer into the B16-BL6 melanoma. Interleukin 4 transfection of tumor resulted in decreased in vivo tumorigenicity that may be related to a host immune response. Further studies to evaluate the host T-cell response to these gene-modified tumors are being investigated.

  4. Characterization of (357439) 2004 BL86 on its close approach to Earth in 2015

    Science.gov (United States)

    Birlan, M.; Popescu, M.; Nedelcu, D. A.; Turcu, V.; Pop, A.; Dumitru, B.; Stevance, F.; Vaduvescu, O.; Moldovan, D.; Rocher, P.; Sonka, A.; Mircea, L.

    2015-09-01

    Context. The potentially hazardous asteroid (PHA) (357439) 2004 BL86 grazed Earth on January 26, 2015 at a distance of about 1.2 million km. This favorable geometry allowed observing it to derive its physical and dynamical parameters. (357439) 2004 BL86 was previously estimated to be a 500-m body. We study it also considering possible mechanisms that might mitigate the effect of asteroids that might become dangerous for Earth. Aims: Physical and dynamical investigations of this peculiar object were performed to be able to characterize this object. Methods: We used spectral observations obtained in the visible (V) using the Isaac Newton Telescope and in the near-infrared (NIR) using the InfraRed Telescope Facility. A complementary photometric survey during two nights was also provided by the Astronomical Observatory Cluj-Feleacu station in Romania. We anlyzed the data using reliable mathematical tools that were previously published under the acronym M4AST. Results: VNIR spectral observations classify (357439) 2004 BL86 as V-type asteroid. The mineralogical analysis reveals its similarities to howardite-eucrite-diogenite meteorites. The band analysis reveals that the object is more similar to a eucritic and howarditic composition, and that it originated from the crust of a large parent body. The analysis yields a mineralogical solution of Wo17Fs39 with an error bar of 4%. Based on the average value of the thermal albedo for V-type objects, its diameter was re-estimated to a value of 290 ± 30 m. The dynamical analysis shows a chaotical behavior of (357439) 2004 BL86. The statistics on meteorite falls show that (357439) 2004 BL86 does not appear to significantly contribute to the current howardite-eucrite-diogenite meteorite flux. For the two photometrical observing runs the following values of the rotational period and peak-to-peak amplitude were estimated for the light curves: 2.637 ± 0.024 h, 0.105 ± 0.007 mag, and 2.616 ± 0.061 h, 0.109 ± 0.018 mag

  5. Virtual file system for PSDS

    Science.gov (United States)

    Runnels, Tyson D.

    1993-01-01

    This is a case study. It deals with the use of a 'virtual file system' (VFS) for Boeing's UNIX-based Product Standards Data System (PSDS). One of the objectives of PSDS is to store digital standards documents. The file-storage requirements are that the files must be rapidly accessible, stored for long periods of time - as though they were paper, protected from disaster, and accumulative to about 80 billion characters (80 gigabytes). This volume of data will be approached in the first two years of the project's operation. The approach chosen is to install a hierarchical file migration system using optical disk cartridges. Files are migrated from high-performance media to lower performance optical media based on a least-frequency-used algorithm. The optical media are less expensive per character stored and are removable. Vital statistics about the removable optical disk cartridges are maintained in a database. The assembly of hardware and software acts as a single virtual file system transparent to the PSDS user. The files are copied to 'backup-and-recover' media whose vital statistics are also stored in the database. Seventeen months into operation, PSDS is storing 49 gigabytes. A number of operational and performance problems were overcome. Costs are under control. New and/or alternative uses for the VFS are being considered.

  6. PKA spectrum file

    Energy Technology Data Exchange (ETDEWEB)

    Kawai, M. [Toshiba Corp., Kawasaki, Kanagawa (Japan). Nuclear Engineering Lab.

    1997-03-01

    In the Japanese Nuclear Data Committee, the PKA/KERMA file containing PKA spectra, KERMA factors and DPA cross sections in the energy range between 10{sup -5} eV and 50 MeV is being prepared from the evaluated nuclear data. The processing code ESPERANT was developed to calculate quantities of PKA, KERMA and DPA from evaluated nuclear data for medium and heavy elements by using the effective single particle emission approximation (ESPEA). For light elements, the PKA spectra are evaluated by the SCINFUL/DDX and EXIFON codes, simultaneously with other neutron cross sections. The DPA cross sections due to charged particle emitted from light elements are evaluated for high neutron energy above 20 MeV. (author)

  7. Effects of BCL oral administation and herbal acupuncture at BL18, BL19 on Liver function changes induced by Alcohol in the mice

    Directory of Open Access Journals (Sweden)

    Sa-Hyun Park

    2002-02-01

    Full Text Available This dissertation was designed to evaluate the effect of BCL(refinded Bambusae Caulis in Liqua-men oral administration and herbal acupuncture on alcohol metabolism and liver function. For this study. mice were damaged by a large quantity of alcohol and received treatment of either BCL 1 mg/kg in oral or BCL 250㎍/kg in herbal acupuncture-BL18 . BL19 bilateral. and then such parameters as GOT. GPT. catalase and superoxide dismustase(CuZn-SOD, Mn-SOD were measured. The results of the experiments were summarized as follows. 1. Compared with control group, the proper degree of alcohol in serum was not significantly differ from oral administration group and herbal acupuncture group. 2. Compared with control group. the activity of GOT in serum was significantly reduced both oral administration and herbal acupuncture group. 3. Compared with control group. the activity of GPT in serum was significantly reduced both oral administration and herbal acupuncture group. 4. The activity of catalase in liver cell tissue, compared with control group. was not sigificantly affected either by oral administration and herbal acupuncture group. 5. The activity of CuZn-SOD in liver cell tissue was not significantly change in herbal acupuncture and oral administration group. The activity of Mn-SOD was significantly increased in oral administration group. while it was not the case in acupuncture group. In conclusion. we consider that BCL oral administration and herbal acupuncture is highly effetive in recovering alcohol metabolism and liver disfunction induced by alcohol.

  8. A high density physical map of chromosome 1BL supports evolutionary studies, map-based cloning and sequencing in wheat

    Czech Academy of Sciences Publication Activity Database

    Philippe, R.; Paux, E.; Bertin, I.; Sourdille, P.; Choulet, F.; Laugier, Ch.; Šimková, Hana; Šafář, Jan; Bellec, A.; Vautrin, S.; Frenkel, Z.; Cattonaro, F.; Magni, F.; Scalabrin, S.; Martis, M.M.; Mayer, K. F. X.; Korol, A.; Berges, H.; Doležel, Jaroslav; Feuillet, C.

    2013-01-01

    Roč. 14, č. 6 (2013) ISSN 1465-6906 Institutional research plan: CEZ:AV0Z50380511 Keywords : chromosome 1BL * evolution * gene space Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 10.465, year: 2013

  9. Gaining trust in emerging markets. Principles of FiBL projects and its cooperation in South East Europe

    OpenAIRE

    Schneider, Monika

    2006-01-01

    FiBL principles, working approach - Respond to local partners requests - Market oriented approach - Promote sustainability of local partners, set up of institutional framework for organic sector development, to develop service provider - Knowledge transfer to local institutions - Long term cooperation

  10. 5 CFR 1203.13 - Filing pleadings.

    Science.gov (United States)

    2010-01-01

    ... delivery, by facsimile, or by e-filing in accordance with § 1201.14 of this chapter. If the document was... submitted by e-filing, it is considered to have been filed on the date of electronic submission. (e... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Filing pleadings. 1203.13 Section 1203.13...

  11. 12 CFR 16.33 - Filing fees.

    Science.gov (United States)

    2010-01-01

    ... Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY SECURITIES OFFERING DISCLOSURE RULES § 16.33 Filing fees. (a) Filing fees must accompany certain filings made under the provisions of this part... Comptroller of the Currency Fees published pursuant to § 8.8 of this chapter. (b) Filing fees must be paid by...

  12. 77 FR 13587 - Combined Notice of Filings

    Science.gov (United States)

    2012-03-07

    .... Applicants: Transcontinental Gas Pipe Line Company. Description: Annual Electric Power Tracker Filing... Company. Description: 2012 Annual Fuel and Electric Power Reimbursement to be effective 4/1/2012. Filed... submits tariff filing per 154.403: Storm Surcharge 2012 to be effective 4/1/2012. Filed Date: 3/1/12...

  13. 75 FR 4689 - Electronic Tariff Filings

    Science.gov (United States)

    2010-01-29

    ... elements ``are required to properly identify the nature of the tariff filing, organize the tariff database... (or other pleading) and the Type of Filing code chosen will be resolved in favor of the Type of Filing...'s wish expressed in its transmittal letter or in other pleadings, the Commission may not review a...

  14. Effects of electroacupuncture at BL33 on detrusor smooth muscle activity in a rat model of urinary retention.

    Science.gov (United States)

    Liu, Xiaoxu; Liu, Kun; Zhi, Mujun; Mo, Qian; Gao, Xinyan; Liu, Zhishun

    2017-12-01

    Detrusor smooth muscle (DSM) underactivity may lead to urinary retention (UR). Electroacupuncture (EA) at BL33 may be effective in improving DSM contractions. This study aimed to investigate: (1) the effect of EA at BL33; and (2) the effect of different manipulation methods at BL33 on the modulation of DSM contractions in UR rats. 30 male Sprague-Dawley rats were anaesthetised with urethane and modelled by urethral outlet obstruction. First, 2 Hz EA at BL33, SP6 and LI4 wasrandomly applied to the UR rats for 5 min to observe the immediate effects (n=10); second, manual acupuncture (MA) (n=10) and 100 Hz EA (n=10) were applied with the same programme. DSM electromyography (EMG) and cystometrogram data were evaluated. (1) 2 Hz EA at BL33 and SP6 significantly increased DSM discharging frequency (0.80±0.10 Hz, P0.05). Compared with SP6, EA at BL33 had greater positive effects on DSM discharging frequency, duration of discharging, and duration of voiding (all P<0.05). (2) No statistically significant differences were shown between MA, 2 Hz EA and 100 Hz EA interventions when stimulating at BL33, SP6 or LI4. EA at BL33 improved DSM contractions to a greater degree than EA at SP6 or LI4. There were no differences in effect when stimulating using 2 Hz EA, 100 Hz EA and MA. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  15. Detecting Malicious Code by Binary File Checking

    Directory of Open Access Journals (Sweden)

    Marius POPA

    2014-01-01

    Full Text Available The object, library and executable code is stored in binary files. Functionality of a binary file is altered when its content or program source code is changed, causing undesired effects. A direct content change is possible when the intruder knows the structural information of the binary file. The paper describes the structural properties of the binary object files, how the content can be controlled by a possible intruder and what the ways to identify malicious code in such kind of files. Because the object files are inputs in linking processes, early detection of the malicious content is crucial to avoid infection of the binary executable files.

  16. Identification of riboflavin: revealing different metabolic characteristics between Escherichia coli BL21(DE3) and MG1655.

    Science.gov (United States)

    Wang, Xinran; Wang, Qian; Qi, Qingsheng

    2015-06-01

    There are many physiological differences between Escherichia coli B and K-12 strains, owing to their different origins. Deeper insight into the metabolic and regulative mechanisms of these strains will inform improved usage of these industrial workhorses. In the present study, we observed that BL21 fermentation broth gradually turned yellow during cultivation. By spectral analysis and liquid chromatography-mass spectrometry identification, we confirmed for the first time that the yellow substance accumulated in the fermentation broth is riboflavin. Comparing the enzyme sequences involved in riboflavin metabolism between BL21 and MG1655, we identified a site mutation on the 115 residue of bifunctional riboflavin kinase/FMN adenylyltransferase (RibF) in BL21. This His115Leu mutation was found to reduce enzyme activity to 55% of that of MG1655, which is probably one reason for riboflavin accumulation in BL21. Quantitative PCR analysis showed that genes of the entire branch of the riboflavin and FAD biosynthesis pathways in BL21 were up-regulated. Several physiological and metabolic characteristics of BL21 and MG1655 were found to be different, and may also be related to the riboflavin accumulation. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  17. Therapeutic efficacy of interleukin-2 activated killer cells against adriamycin resistant mouse B16-BL6 melanoma.

    Science.gov (United States)

    Gautam, S C; Chikkala, N F; Lewis, I; Grabowski, D R; Finke, J H; Ganapathi, R

    1992-01-01

    Development of multidrug-resistance (MDR) remains a major cause of failure in the treatment of cancer with chemotherapeutic agents. In our efforts to explore alternative treatment regimens for multidrug-resistant tumors we have examined the sensitivity of MDR tumor cell lines to lymphokine activated killer (LAK) cells. Adriamycin (ADM) resistant B16-BL6 melanoma, L1210 and P388 leukemic cell lines were tested for sensitivity to lysis by LAK cells in vitro. While ADM-resistant B16-BL6 and L1210 sublines were found to exhibit at least 2-fold greater susceptibility to lysis by LAK cells, sensitivity of ADM-resistant P388 cell was similar to that of parental cells. Since ADM-resistant B16-BL6 cells were efficiently lysed by LAK cells in vitro, the efficacy of therapy with LAK cells against the ADM-resistant B16-BL6 subline in vivo was evaluated. Compared to mice bearing parental B16-BL6 tumor cells, the adoptive transfer of LAK cells and rIL2 significantly reduced formation of experimental metastases (P less than 0.009) and extended median survival time (P less than 0.001) of mice bearing ADM-resistant B16-BL6 tumor cells. Results suggest that immunotherapy with LAK cells and rIL2 may be a useful modality in the treatment of cancers with the MDR phenotype.

  18. Inhibition of the metastatic spread and growth of B16-BL6 murine melanoma by a synthetic matrix metalloproteinase inhibitor.

    Science.gov (United States)

    Chirivi, R G; Garofalo, A; Crimmin, M J; Bawden, L J; Stoppacciaro, A; Brown, P D; Giavazzi, R

    1994-08-01

    The synthetic matrix metalloproteinase inhibitor batimastat was tested for its ability to inhibit growth and metastatic spread of the B16-BL6 murine melanoma in syngeneic C57BL/6N mice. Intraperitoneal administration of batimastat resulted in a significant inhibition in the number of lung colonies produced by B16-BL6 cells injected i.v. The effect of batimastat on spontaneous metastases was examined in mice inoculated in the hind footpad with B16-BL6 melanoma. The primary tumor was removed surgically after 26-28 days. Batimastat was administered twice a day from day 14 to day 28 (pre-surgery) or from day 26 to day 44 (post-surgery). With both protocols, the median number of lung metastases was not significantly affected, but there was a significant reduction in the weight of the metastases. Finally, the effect of batimastat was examined on s.c. growth of B16-BL6 melanoma. Batimastat administered daily, starting at day of tumor transplantation, resulted in a significant growth delay, whereas treatment starting at advanced stage tumor only reduced tumor growth marginally. Our results indicate that a matrix metalloproteinase inhibitor can not only prevent the colonization of secondary organs by B16-BL6 cells but also limit the growth of solid tumors.

  19. 77 FR 74839 - Combined Notice of Filings

    Science.gov (United States)

    2012-12-18

    ..., LP. Description: National Grid LNG, LP submits tariff filing per 154.203: Adoption of NAESB Version 2... with Order to Amend NAESB Version 2.0 Filing to be effective 12/1/2012. Filed Date: 12/11/12. Accession...: Refile to comply with Order on NAESB Version 2.0 Filing to be effective 12/1/2012. Filed Date: 12/11/12...

  20. The File System Interface is an Anachronism

    OpenAIRE

    Ellard, Daniel

    2003-01-01

    Contemporary file systems implement a set of abstractions and semantics that are suboptimal for many (if not most) purposes. The philosophy of using the simple mechanisms of the file system as the basis for a vast array of higher-level mechanisms leads to inefficient and incorrect implementations. We propose several extensions to the canonical file system model, including explicit support for lock files, indexed files, and resource forks, and the benefit of session semantics for write updates...

  1. Curved canals: Ancestral files revisited

    Directory of Open Access Journals (Sweden)

    Jain Nidhi

    2008-01-01

    Full Text Available The aim of this article is to provide an insight into different techniques of cleaning and shaping of curved root canals with hand instruments. Although a plethora of root canal instruments like ProFile, ProTaper, LightSpeed ® etc dominate the current scenario, the inexpensive conventional root canal hand files such as K-files and flexible files can be used to get optimum results when handled meticulously. Special emphasis has been put on the modifications in biomechanical canal preparation in a variety of curved canal cases. This article compiles a series of clinical cases of root canals with curvatures in the middle and apical third and with S-shaped curvatures that were successfully completed by employing only conventional root canal hand instruments.

  2. Value Modifier Public Use File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Center for Medicare (CM) has created a standard analytical file intended to promote transparency. For each Value Modifier performance year, CM will publish a...

  3. Cytoscape file of chemical networks

    Data.gov (United States)

    U.S. Environmental Protection Agency — The maximum connectivity scores of pairwise chemical conditions summarized from Cmap results in a file with Cytoscape format (http://www.cytoscape.org/). The figures...

  4. File: nuclear safety and transparency

    International Nuclear Information System (INIS)

    Martinez, J.P.; Etchegoyen, A.; Jeandron, C.

    2001-01-01

    Several experiences of nuclear safety and transparency are related in this file. Public information, access to documents, transparency in nuclear regulation are such subjects developed in this debate. (N.C.)

  5. Physician Compare National Downloadable File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Physician Compare National Downloadable File is organized at the individual eligible professional level; each line is unique at the professional/enrollment...

  6. Registering Researchers in Authority Files

    NARCIS (Netherlands)

    Altman, M.; Conlon, M.; Cristan, A.L.; Dawson, L.; Dunham, J.; Hickey, T.; Hook, D.; Horstmann, W.; MacEwan, A.; Schreur, P.; Smart, L.; Smith-Yoshimura, K.; Wacker, M.; Woutersen, S.

    2014-01-01

    Registering researchers in some type of authority file or identifier system has become more compelling as both institutions and researchers recognize the need to compile their scholarly output. The report presents functional requirements and recommendations for six stakeholders: researchers,

  7. Health Topic XML File Description

    Science.gov (United States)

    ... this page: https://medlineplus.gov/xmldescription.html Health Topic XML File Description: MedlinePlus To use the sharing ... information categories assigned. Example of a Full Health Topic Record A record for a MedlinePlus health topic ...

  8. JNDC FP decay data file

    International Nuclear Information System (INIS)

    Yamamoto, Tohru; Akiyama, Masatsugu

    1981-02-01

    The decay data file for fission product nuclides (FP DECAY DATA FILE) has been prepared for summation calculation of the decay heat of fission products. The average energies released in β- and γ-transitions have been calculated with computer code PROFP. The calculated results and necessary information have been arranged in tabular form together with the estimated results for 470 nuclides of which decay data are not available experimentally. (author)

  9. Specifying a Realistic File System

    Directory of Open Access Journals (Sweden)

    Sidney Amani

    2015-11-01

    Full Text Available We present the most interesting elements of the correctness specification of BilbyFs, a performant Linux flash file system. The BilbyFs specification supports asynchronous writes, a feature that has been overlooked by several file system verification projects, and has been used to verify the correctness of BilbyFs's fsync( C implementation. It makes use of nondeterminism to be concise and is shallowly-embedded in higher-order logic.

  10. 76 FR 43679 - Filing via the Internet; Notice of Additional File Formats for efiling

    Science.gov (United States)

    2011-07-21

    ... list of acceptable file formats the four-character file extensions for Microsoft Office 2007/2010... files from Office 2007 or Office 2010 in an Office 2003 format prior to submission. Dated: July 15, 2011...

  11. Enhanced glucose tolerance in pancreatic-derived factor (PANDER knockout C57BL/6 mice

    Directory of Open Access Journals (Sweden)

    Shari L. Moak

    2014-11-01

    Full Text Available Pancreatic-derived factor (PANDER; also known as FAM3B is a uniquely structured protein strongly expressed within and secreted from the endocrine pancreas. PANDER has been hypothesized to regulate fasting and fed glucose homeostasis, hepatic lipogenesis and insulin signaling, and to serve a potential role in the onset or progression of type 2 diabetes (T2D. Despite having potentially pivotal pleiotropic roles in glycemic regulation and T2D, there has been limited generation of stable animal models for the investigation of PANDER function, and there are no models on well-established genetic murine backgrounds for T2D. Our aim was to generate an enhanced murine model to further elucidate the biological function of PANDER. Therefore, a pure-bred PANDER knockout C57BL/6 (PANKO-C57 model was created and phenotypically characterized with respect to glycemic regulation and hepatic insulin signaling. The PANKO-C57 model exhibited an enhanced metabolic phenotype, particularly with regard to enhanced glucose tolerance. Male PANKO-C57 mice displayed decreased fasting plasma insulin and C-peptide levels, whereas leptin levels were increased as compared with matched C57BL/6J wild-type mice. Despite similar peripheral insulin sensitivity between both groups, hepatic insulin signaling was significantly increased during fasting conditions, as demonstrated by increased phosphorylation of hepatic PKB/Akt and AMPK, along with mature SREBP-1 expression. Insulin stimulation of PANKO-C57 mice resulted in increased hepatic triglyceride and glycogen content as compared with wild-type C57BL/6 mice. In summary, the PANKO-C57 mouse represents a suitable model for the investigation of PANDER in multiple metabolic states and provides an additional tool to elucidate the biological function and potential role in T2D.

  12. Constraining the red shifts of TeV BL Lac objects

    Science.gov (United States)

    Qin, Longhua; Wang, Jiancheng; Yan, Dahai; Yang, Chuyuan; Yuan, Zunli; Zhou, Ming

    2018-01-01

    We present a model-dependent method to estimate the red shifts of three TeV BL Lac objects (BL Lacs) through fitting their (quasi-)simultaneous multi-waveband spectral energy distributions (SEDs) with a one-zone leptonic synchrotron self-Compton model. Considering the impact of electron energy distributions (EEDs) on the results, we use three types of EEDs to fit the SEDs: a power-law EED with exponential cut-off (PLC), a log-parabola (PLLP) EED and the broken power-law (BPL) EED. We also use a parameter α to describe the uncertainties of the extragalactic background light models, as in Abdo et al. We then use a Markov chain Monte Carlo method to explore the multi-dimensional parameter space and obtain the uncertainties of the model parameters based on the observational data. We apply our method to obtain the red shifts of three TeV BL Lac objects in the marginalized 68 per cent confidence, and find that the PLC EED does not fit the SEDs. For 3C66A, the red shift is 0.14-0.31 and 0.16-0.32 in the BPL and PLLP EEDs. For PKS1424+240, the red shift is 0.55-0.68 and 0.55-0.67 in the BPL and PLLP EEDs. For PG1553+113, the red shift is 0.22-0.48 and 0.22-0.39 in the BPL and PLLP EEDs. We also estimate the red shift of PKS1424+240 in the high stage to be 0.46-0.67 in the PLLP EED, roughly consistent with that in the low stage.

  13. Behavioral architecture of opioid reward and aversion in C57BL/6 substrains

    Directory of Open Access Journals (Sweden)

    Stacey L Kirkpatrick

    2015-01-01

    Full Text Available Drug liking versus drug disliking is a subjective motivational measure in humans that assesses the addiction liability of drugs. Variation in this trait is hypothesized to influence vulnerability versus resilience toward substance abuse disorders and likely contains a genetic component. In rodents and humans, conditioned place preference (CPP / aversion (CPA is a Pavlovian conditioning paradigm whereby a learned preference for the drug-paired environment is used to infer drug liking whereas a learned avoidance or aversion is used to infer drug disliking. C57BL/6 inbred mouse substrains are nearly genetically identical, yet demonstrate robust differences in addiction-relevant behaviors, including locomotor sensitization to cocaine and consumption of ethanol. Here, we tested the hypothesis that B6 substrains would demonstrate differences in the rewarding properties of the mu opioid receptor agonist oxycodone (5 mg/kg, i.p. and the aversive properties of the opioid receptor antagonist naloxone (4 mg/kg, i.p.. Both substrains showed similar degrees of oxycodone-induced CPP; however, there was a three-fold enhancement of naloxone-induced CPA in agonist-naïve C57BL/6J relative to C57Bl/6NJ mice. Exploratory factor analysis of CPP and CPA identified unique factors that explain variance in behavioral expression of reward versus aversion. Conditioned Opioid-Like Behavior was a reward-based factor whereby drug-free locomotor variables resembling opioid treatment co-varied with the degree of CPP. Avoidance and Freezing was an aversion-based factor, whereby the increase in the number of freezing bouts co-varied with the degree of aversion. These results provide new insight into the behavioral architecture of the motivational properties of opioids. Future studies will use quantitative trait locus mapping in B6 substrains to identify novel genetic factors that contribute to the marked strain difference in NAL-CPA.

  14. BROAD Lyα EMISSION FROM THREE NEARBY BL LACERTAE OBJECTS

    International Nuclear Information System (INIS)

    Stocke, John T.; Danforth, Charles W.; Perlman, Eric S.

    2011-01-01

    We present far-UV HST/COS spectra of four nearby BL Lac objects. BL Lac spectra are dominated by a smooth, power-law continuum which arises in a relativistic jet. However, the spectra are not necessarily featureless; weak, broad- and/or narrow-line emission is sometimes seen in high-quality optical spectra. We present detections of Lyα emission in HST/COS spectra of Mrk 421 (z = 0.030) and PKS 2005-489 (z = 0.071) as well as an archival HST/GHRS observation of Mrk 501 (z = 0.0337). Archival HST/STIS observations of PKS 2155-304 (z = 0.116) show no Lyα emission to a very low upper limit. Using the assumption that the broad-line region (BLR) clouds are symmetrically placed around the active galactic nucleus (AGN), we use these measured Lyα emission features to constrain either the relativistic Γ values for the ionizing continuum produced by the jet (in the ionization-bounded case) or the mass of warm gas (in the density-bounded case). While realistic Γ values can be obtained for all four cases, the values for Mrk 421 and PKS 2155-304 are high enough to suggest that covering factors of BLR clouds of ∼1%-2% might be required to provide consistency with earlier values of Doppler boosting and viewing angles suggested for this class of BL Lacs. This discrepancy also exists in the case of M 87, where the amount of Doppler boosting in our direction is expected to be minimal, again suggestive of a small covering factor of BLR clouds. If, as these small covering factors might suggest, the assumptions of a density-bounded model could be more correct, then the observed Lyα luminosities require that BL Lac/FR 1 nuclei possess very little warm gas (10 -4 to 10 -5 M sun ) as suggested by Guilbert et al. If these clouds are in pressure balance with a hotter (∼10 6 K) gas, the BLR contains too little mass to power the AGN by accretion alone.

  15. Withdrawal from Chronic Nicotine Administration Impairs Contextual Fear Conditioning in C57BL/6 Mice

    OpenAIRE

    Davis, Jennifer A.; James, John R.; Siegel, Steven J.; Gould, Thomas J.

    2005-01-01

    The effects of acute nicotine administration (0.09 mg/kg nicotine), chronic nicotine administration (6.3 mg/kg/d nicotine for 14 d), and withdrawal from chronic nicotine administration on fear conditioning in C57BL/6 mice were examined. Mice were trained using two coterminating conditioned stimulus (30 s; 85 dB white noise)– unconditioned stimulus (2 s; 0.57 mA foot shock) pairings and tested 24 h later for contextual and cued fear conditioning. Acute nicotine administration enhanced contextu...

  16. VLBA Observations of Low Luminosity Flat Spectrum Radio Galaxies and BL Lac Objects: Polarisation Properties

    Science.gov (United States)

    Bondi, M.; Dallacasa, D.; Stanghellini, C.; Marchã, M. J. M.

    We obtained two-epoch VLBA observations at 5 GHz of a list of radio galaxies drawn from the 200 mJy sample (Marcha et al. 1996). The objects selected for milli-arcsecond scale observations are classified, on the basis of their optical spectroscopic and polarimetric properties, as BL Lac objects, normal weak line radio galaxies, broad line radio galaxies, and transition objects (those with intermediate properties). We present preliminary results on the radio polarization properties, on the milli-arcsecond scale, of objects with different optical properties and discuss structural variations detected from the two epochs.

  17. Adipocytes Promote B16BL6 Melanoma Cell Invasion and the Epithelial-to-Mesenchymal Transition

    OpenAIRE

    Kushiro, Kyoko; Chu, Randy A.; Verma, Akanksha; Núñez, Nomelí P.

    2011-01-01

    Metastatic melanoma is one of the most deadly and evasive types of cancer. On average, cancer patients with metastatic melanoma survive only 6–9 months after diagnosis. Epidemiological and animal studies suggest that obesity increases the metastatic ability of malignant melanoma, though the mechanism is not known. In the present studies, we assessed the ability of 3T3L1 adipocytes to modulate B16BL6 melanoma cell invasion and the Epithelial-to-Mesenchymal Transition (EMT). For this purpose, w...

  18. Effect of Fenbendazole on Three Behavioral Tests in Male C57BL/6N Mice

    OpenAIRE

    Gadad, Bharathi S; Daher, João P L; Hutchinson, Eric K; Brayton, Cory F; Dawson, Ted M; Pletnikov, Mikhail V; Watson, Julie

    2010-01-01

    Pinworms are highly contagious parasites of laboratory rodents that often are treated with fenbendazole. To our knowledge, the effect of fenbendazole at therapeutic dosages on behavioral tests in mice has not been evaluated. Here we studied 6-wk-old male C57BL/6N mice. We compared the behavior of control mice (fed regular diet) with 3 groups of mice treated with dietary fenbendazole. Treatment groups were 4 wk of fenbendazole, 2 wk of fenbendazole followed by 2 wk of regular diet, and 2 wk of...

  19. Zebra: A striped network file system

    Science.gov (United States)

    Hartman, John H.; Ousterhout, John K.

    1992-01-01

    The design of Zebra, a striped network file system, is presented. Zebra applies ideas from log-structured file system (LFS) and RAID research to network file systems, resulting in a network file system that has scalable performance, uses its servers efficiently even when its applications are using small files, and provides high availability. Zebra stripes file data across multiple servers, so that the file transfer rate is not limited by the performance of a single server. High availability is achieved by maintaining parity information for the file system. If a server fails its contents can be reconstructed using the contents of the remaining servers and the parity information. Zebra differs from existing striped file systems in the way it stripes file data: Zebra does not stripe on a per-file basis; instead it stripes the stream of bytes written by each client. Clients write to the servers in units called stripe fragments, which are analogous to segments in an LFS. Stripe fragments contain file blocks that were written recently, without regard to which file they belong. This method of striping has numerous advantages over per-file striping, including increased server efficiency, efficient parity computation, and elimination of parity update.

  20. NLO+NLL collider bounds, Dirac fermion and scalar dark matter in the B-L model

    Energy Technology Data Exchange (ETDEWEB)

    Klasen, Michael [Westfaelische Wilhelms-Universitaet Muenster, Institut fuer Theoretische Physik, Muenster (Germany); Lyonnet, Florian [Southern Methodist University, Dallas, TX (United States); Queiroz, Farinaldo S. [Max-Planck-Institut fuer Kernphysik, Particle and Astroparticle Physics Division, Heidelberg (Germany)

    2017-05-15

    Baryon and lepton numbers being accidental global symmetries of the Standard Model (SM), it is natural to promote them to local symmetries. However, to preserve anomaly-freedom, only combinations of B-L are viable. In this spirit, we investigate possible dark matter realizations in the context of the U(1){sub B-L} model: (i) Dirac fermion with unbroken B-L; (ii) Dirac fermion with broken B-L; (iii) scalar dark matter; (iv) two-component dark matter. We compute the relic abundance, direct and indirect detection observables and confront them with recent results from Planck, LUX-2016, and Fermi-LAT and prospects from XENON1T. In addition to the well-known LEP bound M{sub Z}{sup {sub '}}/g{sub BL} >or similar 7 TeV, we include often ignored LHC bounds using 13 TeV dilepton (dimuon + dielectron) data at next-to-leading order plus next-to-leading logarithmic accuracy. We show that, for gauge couplings smaller than 0.4, the LHC gives rise to the strongest collider limit. In particular, we find M{sub Z}{sup {sub '}}/g{sub BL} > 8.7 TeV for g{sub BL} = 0.3. We conclude that the NLO+NLL corrections improve the dilepton bounds on the Z{sup '} mass and that both dark matter candidates are only viable in the Z{sup '} resonance region, with the parameter space for scalar dark matter being fully probed by XENON1T. Lastly, we show that one can successfully have a minimal two-component dark matter model. (orig.)

  1. Sub-Hour X-Ray Variability of High-Energy Peaked BL Lacertae Objects

    Directory of Open Access Journals (Sweden)

    Bidzina Kapanadze

    2018-03-01

    Full Text Available The study of multi-wavelength flux variability in BL Lacertae objects is very important to discern unstable processes and emission mechanisms underlying their extreme observational features. While the innermost regions of these objects are not accessible from direct observations, we may draw conclusions about their internal structure via the detection of flux variations on various timescales, based on the light-travel argument. In this paper, we review the sub-hour X-ray variability in high-energy peaked BL Lacertae sources (HBLs that are bright at X-rays and provide us with an effective tool to study the details related to the physics of the emitting particles. The X-ray emission of these sources is widely accepted to be a synchrotron radiation from the highest-energy electrons, and the complex spectral variability observed in this band reflects the injection and radiative evolution of freshly-accelerated particles. The detection of sub-hour X-ray flux variability is very important since it can be related to the small-scale jet turbulent structures or triggered by unstable processes occurring in the vicinity of a central supermassive black hole. We summarize the fastest X-ray variability instances detected in bright HBLs and discuss their physical implications.

  2. Protein crystallography beamline (PX-BL21); its utilization and research highlights

    International Nuclear Information System (INIS)

    Kumar, Ashwani; Ghosh, Biplab; Singh, Rahul; Makde, Ravindra; Sharma, Surinder M.

    2016-01-01

    The protein crystallography beamline (PX-BL21) is sourced on 1.5 T bending magnet of 2.5 GeV Indus-2 synchrotron. This beamline has been designed to perform monochromatic and anomalous diffraction experiments on single crystals of biological macromolecules such as protein, DNA and their complexes. PX beamline also has a state-of-art ancillary biochemical laboratory to prepare single crystals of biological macromolecules. Since the commissioning of the beamline, it has been utilized by more than 70% of research groups working in the area of protein crystallography in India. About 30 crystal structures of proteins, determined using this beamline, have been deposited in Protein Data Bank (PDB). Some of these structures have been determined using experimental phasing, such as the single wavelength anomalous diffraction (SAD) experiments. The energy tunability of the synchrotron have been exploited to carry our various SAD experiments: Selenium-SAD, Zinc-SAD and Manganese-SAD and Sulphar-SAD. In the present talk, the key results from the PX-BL21 beamline will be discussed. (author)

  3. Cis-bifenthrin induces immunotoxicity in adolescent male C57BL/6 mice.

    Science.gov (United States)

    Wang, Xia; Gao, Xingli; He, Bingnan; Zhu, Jiawei; Lou, Huihui; Hu, Qinglian; Jin, Yuanxiang; Fu, Zhengwei

    2017-07-01

    Bifenthrin (BF) is an important synthetic pyrethroid. Previous studies have demonstrated that cis-BF exhibits toxic effects on development, the neurological, reproductive and endocrine system. In this study, we evaluated the immunotoxicity caused by cis-BF in adolescent male C57BL/6 mice. Mice were exposed orally to 0, 5, 10, and 20 mg/kg/d for 3 weeks. The results showed that body weight, spleen weight, and splenic cellularity decreased in mice exposed to 20 mg/kg/d cis-BF. Additionally, we found that the mRNA levels of the pro-inflammatory factors IL-1β, IL-6, CXCL-1, and TNF-α, in peritoneal macrophages, the spleen, and the thymus were inhibited in the cis-BF-treated groups. Moreover, MTT assays demonstrated that cis-BF inhibited splenocyte proliferation stimulated by LPS or Con A, as well as the secretion of IFN-γ on Con A stimulation. Collectively, the results of this study suggest that exposure to cis-BF has the potential to induce immunotoxicity in adolescent male C57BL/6 mice. © 2017 Wiley Periodicals, Inc.

  4. Radio emission from quasars and BL Lac objects by coherent plasma oscillation and stimulated Compton scattering

    International Nuclear Information System (INIS)

    Colgate, S.A.; Petschek, A.G.

    1978-01-01

    The full radiation spectrum of quasars and BL Lac objects is interpreted as due to a dependent combination of a soft plasma oscillation source at 2ν/sub P/ and bremsstrahlung. Previous work of the plasma oscillation radiation is extended into the radio part of the spectrum and it is shown how the high brightness temperature observations of BL Lac objects [kT/sub b/ (100 MHz) approximate = 3 x 10 5 mc 2 ] are a reasonable consequence of a lower external plasma density and ejection as required for the observed lack of emission lines. Two extreme cases are considered, the one where the plasma oscillations are suddenly extinguished and only stimulated Compton scattering remains and a second case of a constant source of plasma oscillations but a graded surface density. The first case gives 1/100 of the required brightness temperature and the second gives 100 times too large a brightness temperature and also a x 10 too large a radius. It is believed reasonable to invoke a combination of both processes to explain the observed radio spectrum. This model circumvents the self-Compton x-ray flux difficulty of incoherent synchrotron emission

  5. Soft X-ray observations of two BL Lacertae objects - markarian 421 and 501

    International Nuclear Information System (INIS)

    Singh, K.P.; Garmire, G.P.

    1985-01-01

    This paper reports on the soft X-ray (0.15-2.8 keV) observations of two BL Lacertae-type objects, viz., Mrk 421 and Mrk 501. The observations were made with the low-energy detectors on the HEAO 1 satellite during the period 1977 August-1978 December. Steep, soft X-ray power-law spectra with photon index Gamma = 3 are found for both Mrk 421 and Mrk 501. The power-law models are found to give a significantly better fit than the thermal models to the observed pulse-height spectra of Mrk 421 and Mrk 501. Day-to-day soft X-ray (0.25 keV band) intensity variations are observed only in Mrk 501. No significant change is found in Gamma from both the BL Lac objects during the period of observations. However, the sum of all the X-ray observations from 1976 until 1980 can be understood in terms of two spectral components of variable intensity to account for the X-ray emission observed between 0.15 and 20 keV from Mrk 421 and Mrk 501. 24 references

  6. Ultrasonic assisted dyeing: dyeing of acrylic fabrics C.I. Astrazon Basic Red 5BL 200%.

    Science.gov (United States)

    Kamel, M M; Helmy, H M; Mashaly, H M; Kafafy, H H

    2010-01-01

    The dyeing of acrylic fabrics using C.I. Astrazon Basic Red 5BL 200% has been studied with both conventional and ultrasonic techniques. The effect of dye concentration, dye bath pH, ultrasonic power, dyeing time and temperature were studied and the resulting shades obtained by dyeing with both techniques were compared. Colour strength values obtained were found to be higher with ultrasonic than with conventional heating. The results of fastness properties of the dyed fabrics were studied. X-ray and Scanning Electron Microscope SEM were carried out on dyed samples using both methods of dyeing to find out an explanation for the better dyeability of acrylic fabrics with (US) method. Dyeing kinetics of acrylic fabrics using C.I. Astrazon Basic Red 5BL 200% using conventional and ultrasonic conditions were compared. The time/dye-uptake isotherms are revealing the enhanced dye-uptake in the second phase of dyeing. The values of dyeing rate constant, half-time of dyeing and standard affinity and ultrasonic efficiency have been calculated and discussed.

  7. KEEFEKTIFAN MODEL PjBL DENGAN TUGAS CREATIVE MIND-MAP UNTUK MENINGKATKAN KONEKSI MATEMATIK SISWA

    Directory of Open Access Journals (Sweden)

    Zulfa Ainurrizqiyah

    2015-08-01

    Full Text Available Tujuan  penelitian ini adalah untuk mengetahui  peningkatan kemampuan koneksi matematik, apakah terdapat perbedaan serta manakah yang lebih baik antara pembelajaran dengan model PjBL dengan tugas creative mind-map dan pembelajaran ekspositori pada siswa kelas X materi Trigonometri. Penelitian eksperimen yang dilakukan dengan pre and posttest control group design ini memiliki populasi yakni siswa kelas X SMAN 1 Sukorejo tahun ajaran 2014/2015. Dengan menggunakan cluster random sampling, terpilih sampel yaitu siswa kelas XB sebagai kelas kontrol dengan pembelajaran ekspositori dan XC sebagai kelas eksperimen dengan model PjBL dengan tugas creative mind-map. Data kemampuan koneksi matematik dianalisis menggunakan uji proporsi, uji beda rata-rata. Berdasarkan uji proporsi, diperoleh lebih dari 75 % siswa kelas eksperimen mencapai nilai ketuntasan belajar, yaitu 75. Selain itu, diperoleh adanya perbedaan hasil kemampuan koneksi matematik antar kedua kelas dimana kelas eksperimen lebih baik daripada siswa kelas kontrol. Sedangkan untuk peningkatan menggunakan uji t berpasangan dan gain ternormalisasi dengan hasilnya yaitu terdapat peningkatan kemampuan koneksi matematik pada kelas eksperimen dengan kriteria tinggi.

  8. Double crystal monochromator controlled by integrated computing on BL07A in New SUBARU, Japan

    Energy Technology Data Exchange (ETDEWEB)

    Okui, Masato, E-mail: okui@kohzu.co.jp [Kohzu Precision Co., Ltd., 2-6-15, Kurigi, Asao-ku, Kawasaki-shi, Kanagawa 215-8521 (Japan); Laboratory of Advanced Science and Technology for Industry, University of Hyogo (Japan); Yato, Naoki; Watanabe, Akinobu; Lin, Baiming; Murayama, Norio [Kohzu Precision Co., Ltd., 2-6-15, Kurigi, Asao-ku, Kawasaki-shi, Kanagawa 215-8521 (Japan); Fukushima, Sei, E-mail: FUKUSHIMA.Sei@nims.go.jp [Laboratory of Advanced Science and Technology for Industry, University of Hyogo (Japan); National Institute for Material Sciences (Japan); Kanda, Kazuhiro [Laboratory of Advanced Science and Technology for Industry, University of Hyogo (Japan)

    2016-07-27

    The BL07A beamline in New SUBARU, University of Hyogo, has been used for many studies of new materials. A new double crystal monochromator controlled by integrated computing was designed and installed in the beamline in 2014. In this report we will discuss the unique features of this new monochromator, MKZ-7NS. This monochromator was not designed exclusively for use in BL07A; on the contrary, it was designed to be installed at low cost in various beamlines to facilitate the industrial applications of medium-scale synchrotron radiation facilities. Thus, the design of the monochromator utilized common packages that can satisfy the wide variety of specifications required at different synchrotron radiation facilities. This monochromator can be easily optimized for any beamline due to the fact that a few control parameters can be suitably customized. The beam offset can be fixed precisely even if one of the two slave axes is omitted. This design reduces the convolution of mechanical errors. Moreover, the monochromator’s control mechanism is very compact, making it possible to reduce the size of the vacuum chamber can be made smaller.

  9. Respiratory syncytial virus increases lung cellular bioenergetics in neonatal C57BL/6 mice

    International Nuclear Information System (INIS)

    Alsuwaidi, Ahmed R.; Albawardi, Alia; Almarzooqi, Saeeda; Benedict, Sheela; Othman, Aws R.; Hartwig, Stacey M.; Varga, Steven M.; Souid, Abdul-Kader

    2014-01-01

    We have previously reported that lung cellular bioenergetics (cellular respiration and ATP) increased in 4–10 week-old BALB/c mice infected with respiratory syncytial virus (RSV). This study examined the kinetics and changes in cellular bioenergetics in ≤2-week-old C57BL/6 mice following RSV infection. Mice (5–14 days old) were inoculated intranasally with RSV and the lungs were examined on days 1–10 post-infection. Histopathology and electron microscopy revealed preserved pneumocyte architectures and organelles. Increased lung cellular bioenergetics was noted from days 1–10 post-infection. Cellular GSH remained unchanged. These results indicate that the increased lung cellular respiration (measured by mitochondrial O 2 consumption) and ATP following RSV infection is independent of either age or genetic background of the host. - Highlights: • RSV infection increases lung cellular respiration and ATP in neonatal C57BL/6 mice. • Increased lung cellular bioenergetics is a biomarker of RSV infection. • Lung cellular glutathione remains unchanged in RSV infection

  10. Spontaneous B-L breaking as the origin of the hot early universe

    Energy Technology Data Exchange (ETDEWEB)

    Buchmueller, W.; Domcke, V.; Schmitz, K.

    2012-03-15

    The decay of a false vacuum of unbroken B-L symmetry is an intriguing and testable mechanism to generate the initial conditions of the hot early universe. If B-L is broken at the grand unification scale, the false vacuum phase yields hybrid inflation, ending in tachyonic preheating. The dynamics of the B - L breaking Higgs field and thermal processes produce an abundance of heavy neutrinos whose decays generate entropy, baryon asymmetry and gravitino dark matter. We study the phase transition for the full supersymmetric Abelian Higgs model. For the subsequent reheating process we give a detailed time-resolved description of all particle abundances. The competition of cosmic expansion and entropy production leads to an intermediate period of constant 'reheating' temperature, during which baryon asymmetry and dark matter are produced. Consistency of hybrid inflation, leptogenesis and gravitino dark matter implies relations between neutrino parameters and superparticle masses, in particular a lower bound on the gravitino mass of 10 GeV.

  11. An Analysis of Periodic Components in BL Lac Object S5 0716 +714 with MUSIC Method

    Science.gov (United States)

    Tang, J.

    2012-01-01

    Multiple signal classification (MUSIC) algorithms are introduced to the estimation of the period of variation of BL Lac objects.The principle of MUSIC spectral analysis method and theoretical analysis of the resolution of frequency spectrum using analog signals are included. From a lot of literatures, we have collected a lot of effective observation data of BL Lac object S5 0716 + 714 in V, R, I bands from 1994 to 2008. The light variation periods of S5 0716 +714 are obtained by means of the MUSIC spectral analysis method and periodogram spectral analysis method. There exist two major periods: (3.33±0.08) years and (1.24±0.01) years for all bands. The estimation of the period of variation of the algorithm based on the MUSIC spectral analysis method is compared with that of the algorithm based on the periodogram spectral analysis method. It is a super-resolution algorithm with small data length, and could be used to detect the period of variation of weak signals.

  12. Minocycline does not evoke anxiolytic and antidepressant-like effects in C57BL/6 mice.

    Science.gov (United States)

    Vogt, M A; Mallien, A S; Pfeiffer, N; Inta, I; Gass, P; Inta, D

    2016-03-15

    Minocycline is a broad-spectrum tetracycline antibiotic with multiple actions, including anti-inflammatory and neuroprotective effects, that was proposed as novel treatment for several psychiatric disorders including schizophrenia and depression. However, there are contradictory results regarding antidepressant effects of minocycline in rodent models. Additionally, the possible anxiolytic effect of minocycline is still poorly investigated. Therefore, we aimed to clarify in the present study the influence of minocycline on behavioral correlates of mood disorders in standard tests for depression and anxiety, the Porsolt Forced Swim Test (FST), Elevated O-Maze, Dark-Light Box Test and Openfield Test in adult C57BL/6 mice. We found, unexpectedly, that mice treated with minocycline (20-40mg/kg, i.p.) did not display antidepressant- or anxiolytic-like behavioral changes in contrast to mice treated with diazepam (0.5mg/kg, anxiety tests) or imipramine (20mg/kg, depressive-like behavior). These results are relevant for future studies, considering that C57BL/6 mice, the most widely used strain in pharmacological and genetic animal models, did not react as expected to the treatment regime applied. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. Alcohol consumption suppresses metastasis of B16-BL6 melanoma in mice.

    Science.gov (United States)

    Meadows, G G; Elstad, C A; Blank, S E; Gallucci, R M; Pfister, L J

    1993-03-01

    Female C57BL/6 mice were fed a defined, pelleted diet and given 10% w/v or 20% w/v ethanol in their drinking water. Natural killer (NK) cell cytolytic activity was compared between water-drinking and ethanol-consuming mice and in mice that were also treated with polyinosinic-polycytidylic acid (poly I:C) to augment NK cell activity or with anti-NK1.1 antibody to decrease activity. NK cell cytolytic activity was not altered in mice given 10% ethanol, but was decreased in mice given 20% ethanol compared to water-drinking mice. Poly I:C treatment increased and anti-NK1.1 antibody treatment decreased NK cell activity in both water-drinking and 20% ethanol-consuming mice. Experimental and spontaneous metastases of B16-BL6 melanoma were evaluated as a function of the duration of ethanol consumption before tumor inoculation and as a function of altered NK cell activity. Experimental metastasis was inhibited after 4 and also after 6.5 weeks of ethanol exposure. Poly I:C treatment inhibited tumor lung colonization irrespective of ethanol consumption. Anti-NK1.1 antibody treatment increased metastasis, although to a lesser degree in mice consuming 10% ethanol. Spontaneous metastasis was inhibited in mice consuming 10% ethanol for 4 weeks, and in mice consuming 20% ethanol for 1 and 4 weeks before melanoma inoculation.

  14. Anomalous energy exchange in the gBL and quasilinear theories

    International Nuclear Information System (INIS)

    Mynick, H.E.

    1992-02-01

    The rate of turbulence-induced energy exchange W o between species is computed in the framework of the quasilinear and gBL transport theories, and the relationship between these two theories, and the relationship between these two similar theories is thereby elucidated. For both theories, general formal expressions for W o are developed, and then applied to the trapped electron mode for illustration. The general expressions for W o in the two theories are formally closely related, but can yield predictions of very different magnitude in concrete applications. The fact that quasilinear theory is not valid for saturated steady-state turbulence gives rise to certain peculiarities in its predictions for this normal experimental situation, such as permitting energy to flow from the cooler to the hotter species, even in the limit of thermal equilibrium, where real-space gradients vanish. The gBL theory may be viewed as a modification of quasilinear theory to be valid for steady-state turbulence, keeping extra terms due to the self-consistent back reaction of particles on the fluctuations, which are just such as to eliminate these peculiarities

  15. Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice.

    Science.gov (United States)

    Li, Lin; Mauric, Veronika; Zheng, Jun-Fang; Kang, Sung Ung; Patil, Sudarshan; Höger, Harald; Lubec, Gert

    2010-08-01

    Information on systematic analysis of olfactory memory-related proteins is poor. In this study, the odor discrimination task to investigate olfactory recognition memory of adult male C57BL/6J mice was used. Subsequently, olfactory bulbs (OBs) were taken, proteins extracted, and run on two-dimensional gel electrophoresis with in-gel-protein digestion, followed by mass spectrometry and quantification of differentially expressed proteins. Dual specificity mitogen-activated protein kinase kinase 1 (MEK1), dihydropyrimidinase-related protein 1 (DRP1), and fascin are related with Lemon odor memory. Microtubule-associated protein RP/EB family member 3 is related to Rose odor memory. Hypoxanthine-guanine phosphoribosyltransferase is related with both Lemon and Rose odors memory. MEK1 and DRP1 levels were increased, while microtubule-associated protein RP/EB family member 3, fascin and hypoxanthine-guanine phosphoribosyltransferase levels were decreased during olfactory memory. In summary, neurogenesis, signal transduction, cytoskeleton, and nucleotide metabolism are involved in olfactory memory formation and storage of C57BL/6J mice.

  16. Respiratory syncytial virus increases lung cellular bioenergetics in neonatal C57BL/6 mice

    Energy Technology Data Exchange (ETDEWEB)

    Alsuwaidi, Ahmed R., E-mail: alsuwaidia@uaeu.ac.ae [Departments of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain (United Arab Emirates); Albawardi, Alia, E-mail: alia.albawardi@uaeu.ac.ae [Departments of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain (United Arab Emirates); Almarzooqi, Saeeda, E-mail: saeeda.almarzooqi@uaeu.ac.ae [Departments of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain (United Arab Emirates); Benedict, Sheela, E-mail: sheela.benedict@uaeu.ac.ae [Departments of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain (United Arab Emirates); Othman, Aws R., E-mail: aws.rashad@uaeu.ac.ae [Departments of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain (United Arab Emirates); Hartwig, Stacey M., E-mail: stacey-hartwig@uiowa.edu [Department of Microbiology, Department of Pathology and Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242 (United States); Varga, Steven M., E-mail: steven-varga@uiowa.edu [Department of Microbiology, Department of Pathology and Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242 (United States); Souid, Abdul-Kader, E-mail: asouid@uaeu.ac.ae [Departments of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain (United Arab Emirates)

    2014-04-15

    We have previously reported that lung cellular bioenergetics (cellular respiration and ATP) increased in 4–10 week-old BALB/c mice infected with respiratory syncytial virus (RSV). This study examined the kinetics and changes in cellular bioenergetics in ≤2-week-old C57BL/6 mice following RSV infection. Mice (5–14 days old) were inoculated intranasally with RSV and the lungs were examined on days 1–10 post-infection. Histopathology and electron microscopy revealed preserved pneumocyte architectures and organelles. Increased lung cellular bioenergetics was noted from days 1–10 post-infection. Cellular GSH remained unchanged. These results indicate that the increased lung cellular respiration (measured by mitochondrial O{sub 2} consumption) and ATP following RSV infection is independent of either age or genetic background of the host. - Highlights: • RSV infection increases lung cellular respiration and ATP in neonatal C57BL/6 mice. • Increased lung cellular bioenergetics is a biomarker of RSV infection. • Lung cellular glutathione remains unchanged in RSV infection.

  17. PFS: a distributed and customizable file system

    OpenAIRE

    Bosch, H.G.P.; Mullender, Sape J.

    1996-01-01

    In this paper we present our ongoing work on the Pegasus File System (PFS), a distributed and customizable file system that can be used for off-line file system experiments and on-line file system storage. PFS is best described as an object-oriented component library from which either a true file system or a file-system simulator can be constructed. Each of the components in the library is easily replaced by another implementation to accommodate a wide range of applications.

  18. Spontaneous autoimmunity in 129 and C57BL/6 mice-implications for autoimmunity described in gene-targeted mice.

    Directory of Open Access Journals (Sweden)

    Anne E Bygrave

    2004-08-01

    Full Text Available Systemic lupus erythematosus (SLE is a multisystem autoimmune disorder in which complex genetic factors play an important role. Several strains of gene-targeted mice have been reported to develop SLE, implicating the null genes in the causation of disease. However, hybrid strains between 129 and C57BL/6 mice, widely used in the generation of gene-targeted mice, develop spontaneous autoimmunity. Furthermore, the genetic background markedly influences the autoimmune phenotype of SLE in gene-targeted mice. This suggests an important role in the expression of autoimmunity of as-yet-uncharacterised background genes originating from these parental mouse strains. Using genome-wide linkage analysis, we identified several susceptibility loci, derived from 129 and C57BL/6 mice, mapped in the lupus-prone hybrid (129 x C57BL/6 model. By creating a C57BL/6 congenic strain carrying a 129-derived Chromosome 1 segment, we found that this 129 interval was sufficient to mediate the loss of tolerance to nuclear antigens, which had previously been attributed to a disrupted gene. These results demonstrate important epistatic modifiers of autoimmunity in 129 and C57BL/6 mouse strains, widely used in gene targeting. These background gene influences may account for some, or even all, of the autoimmune traits described in some gene-targeted models of SLE.

  19. Pengaruh Pembelajaran Project Based Learning (PjBL pada Materi Ekosistem terhadap Sikap dan Hasil Belajar Siswa SMAN 2 Malang

    Directory of Open Access Journals (Sweden)

    Susriyati Mahanal

    2010-05-01

    Full Text Available The objective of this research was to know the effect of Project Based Learning (PjBL to the attitude and student cognitive achievement in SMAN 2 Malang. This research applied quasy experimental with non-equivalent control group design. The populations of this research is all student of class X SMAN 2 Malang on academic year 2008/2009. The samples of this research were class X7 as experimental (PjBL and class X8 as control (conventional. The instrument of this research namely cognitive achievement test and attitude scale questionnaire to river ecosystem. This research data are quantitative data consisted by score cognitive achievement and attitude scale questionnaire to river ecosystem, collected on 14 Maret until 31 May 2009. Data were analysed by bilinear covariant statistical analysis (Ancova, continued with LSD test. The result of this research indicated that there were PjBL toward the cognitive achievement and student attitude to river ecosystem. Students with PjBl having attitude higher 11,65% from conventional students. Beside, students with PjBl having cognitive achievement higher 81,05% from conventional students. Based on this research, the researcher suggest to the teacher that this strategy can be implemented in biology learning.   Kata kunci: Project Based Learning, sikap terhadap ekosistem sungai, hasil belajar

  20. Search the SEC website for the latest EDGAR filings

    Data.gov (United States)

    Securities and Exchange Commission — This listing contains the most recent filings for the current official filing date (including filings made after the 5:30pm deadline on the previous filing day)....

  1. Building a parallel file system simulator

    International Nuclear Information System (INIS)

    Molina-Estolano, E; Maltzahn, C; Brandt, S A; Bent, J

    2009-01-01

    Parallel file systems are gaining in popularity in high-end computing centers as well as commercial data centers. High-end computing systems are expected to scale exponentially and to pose new challenges to their storage scalability in terms of cost and power. To address these challenges scientists and file system designers will need a thorough understanding of the design space of parallel file systems. Yet there exist few systematic studies of parallel file system behavior at petabyte- and exabyte scale. An important reason is the significant cost of getting access to large-scale hardware to test parallel file systems. To contribute to this understanding we are building a parallel file system simulator that can simulate parallel file systems at very large scale. Our goal is to simulate petabyte-scale parallel file systems on a small cluster or even a single machine in reasonable time and fidelity. With this simulator, file system experts will be able to tune existing file systems for specific workloads, scientists and file system deployment engineers will be able to better communicate workload requirements, file system designers and researchers will be able to try out design alternatives and innovations at scale, and instructors will be able to study very large-scale parallel file system behavior in the class room. In this paper we describe our approach and provide preliminary results that are encouraging both in terms of fidelity and simulation scalability.

  2. Chronic Condition Public Use File (PUF)

    Data.gov (United States)

    U.S. Department of Health & Human Services — This release contains the Chronic Conditions Public Use Files (PUF) with information from Medicare claims. The CMS Chronic Conditions PUF is an aggregated file in...

  3. Improving File System Performance by Striping

    Science.gov (United States)

    Lam, Terance L.; Kutler, Paul (Technical Monitor)

    1998-01-01

    This document discusses the performance and advantages of striped file systems on the SGI AD workstations. Performance of several striped file system configurations are compared and guidelines for optimal striping are recommended.

  4. 47 CFR 1.1704 - Station files.

    Science.gov (United States)

    2010-10-01

    ... System (COALS) § 1.1704 Station files. Applications, notifications, correspondence, electronic filings... Television Relay Service (CARS) are maintained by the Commission in COALS and the Public Reference Room...

  5. source files for manuscript in tex format

    Data.gov (United States)

    U.S. Environmental Protection Agency — Source tex files used to create the manuscript including original figure files and raw data used in tables and inline text. This dataset is associated with the...

  6. 75 FR 66748 - Notice of Baseline Filings

    Science.gov (United States)

    2010-10-29

    ...- 000] Notice of Baseline Filings October 22, 2010. ONEOK Gas Transportation, L.L.C Docket No. PR11-68... above submitted a revised baseline filing of their Statement of Operating Conditions for services...

  7. Physician-Supplier Procedure Summary Master File

    Data.gov (United States)

    U.S. Department of Health & Human Services — This file is a 100 percent summary of all Part B Carrier and DMERC Claims processed through the Common Working File and stored in the National Claims History...

  8. Data_files_Reyes_EHP_phthalates

    Data.gov (United States)

    U.S. Environmental Protection Agency — The file contains three files in comma separated values (.csv) format. “Reyes_EHP_Phthalates_US_metabolites.csv” contains information about the National Health and...

  9. Ambulance Reasonable Charge Public Use Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Ambulance Reasonable Charge public use files for calendar years (CY) 2003 through 2005 are located in the Downloads section below. These public use files are...

  10. 49 CFR 564.5 - Information filing; agency processing of filings.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Information filing; agency processing of filings... HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION REPLACEABLE LIGHT SOURCE INFORMATION (Eff. until 12-01-12) § 564.5 Information filing; agency processing of filings. (a) Each manufacturer...

  11. Cut-and-Paste file-systems: integrating simulators and file systems

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.

    1995-01-01

    We have implemented an integrated and configurable file system called the Pegasus filesystem (PFS) and a trace-driven file-system simulator called Patsy. Patsy is used for off-line analysis of file-systemalgorithms, PFS is used for on-line file-systemdata storage. Algorithms are first analyzed in

  12. Cut-and-Paste file-systems : integrating simulators and file systems

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.

    1996-01-01

    We have implemented an integrated and configurable file system called the PFS and a trace-driven file-system simulator called Patsy. Patsy is used for off-line analysis of file-system algorithms, PFS is used for on-line file-system data storage. Algorithms are first analyzed in Patsy and when we are

  13. Data on morphometric analysis of the pancreatic islets from C57BL/6 and BALB/c mice

    Directory of Open Access Journals (Sweden)

    Thiago Aparecido da Silva

    2016-09-01

    Full Text Available The endocrine portion of the pancreas, which is characterized by pancreatic islets, has been widely investigated among different species. The BALB/c and C57BL/6 mice are extensively used in experimental research, and the morphometric differences in the pancreatic islets of these animals have not been evaluated so far. Thus, our data have a comparative perspective related to the morphometric analysis of area, diameters, circularity, and density of pancreatic islets from BALB/c and C57BL/6 mice. The data presented here are focused to evaluate the differences in morphology of pancreatic islets of two common laboratory mouse strains. Keywords: Pancreatic islets, Morphometry, BALB/c and C57BL/6 mice

  14. Antimetastatic effect of PSK, a protein-bound polysaccharide, against the B16-BL6 mouse melanoma.

    Science.gov (United States)

    Matsunaga, K; Ohhara, M; Oguchi, Y; Iijima, H; Kobayashi, H

    1996-01-01

    We examined the effect of PSK, a protein-bound polysaccharide, upon in vivo metastasis and in vitro invasion of the B16-BL6 mouse melanoma cells. (1) PSK suppressed in vivo artificial and spontaneous lung metastases of B16-BL6 in C57BL/6 mice. (2) PSK in a dose-dependent fashion suppressed in vitro invasion and chemotaxis of the tumor cells using filters coated with a reconstituted basement membrane. (3) PSK had little effect on DNA synthesis in tumor cells in vitro, but suppressed tumor cell adhesion to, degradation of, and haptotaxis to components of the basement membrane. (4) PSK suppressed the binding of tumor cells to components of the basement membrane. These findings suggest that PSK may suppress metastasis through inhibition of tumor cell invasion and that this effect is the result of interactions between PSK and components of the basement membrane.

  15. Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model

    DEFF Research Database (Denmark)

    Fontenete, Sílvia; Leite, Marina; Cappoen, Davie

    2016-01-01

    ). Finally, the efficiency of FIVH to detect H. pylori SS1 strain in C57BL/6 infected mice was evaluated ex vivo in mucus samples, in cryosections and paraffin-embedded sections by epifluorescence and confocal microscopy. RESULTS: H. pylori SS1 strain infecting C57BL/6 mice was successfully detected...... by the Cy3_HP_LNA/2OMe_PS probe in the mucus, attached to gastric epithelial cells and colonizing the gastric pits. The specificity of the probe for H. pylori was confirmed by microscopy. CONCLUSIONS: In the future this methodology can be used in combination with a confocal laser endomicroscope for in vivo......INTRODUCTION: In this study, we applied fluorescence in vivo hybridization (FIVH) using locked nucleic acid (LNA) probes targeting the bacterial rRNA gene for in vivo detection of H. pylori infecting the C57BL/6 mouse model. A previously designed Cy3_HP_LNA/2OMe_PS probe, complementary...

  16. The Crystallographic Information File (CIF

    Directory of Open Access Journals (Sweden)

    I D Brown

    2006-11-01

    Full Text Available The Crystallographic Information File (CIF, owned by the International Union of Crystallography, is a file structure based on tag-value ASCII pairs with tags defined in machine-readable dictionaries. The crystallographic community publishes and archives large quantities of numeric information generated by crystal structure determinations, and CIF's acceptance was assured by its adoption as the submission format for Acta Crystallographica and by the obvious needs of the community. CIF's strength lies in its dictionaries, which define most of the concepts of crystallography; its weakness is the difficulty of writing software that exploits its full potential.

  17. FileMaker Pro 9

    CERN Document Server

    Coffey, Geoff

    2007-01-01

    FileMaker Pro 9: The Missing Manual is the clear, thorough and accessible guide to the latest version of this popular desktop database program. FileMaker Pro lets you do almost anything with the information you give it. You can print corporate reports, plan your retirement, or run a small country -- if you know what you're doing. This book helps non-technical folks like you get in, get your database built, and get the results you need. Pronto.The new edition gives novices and experienced users the scoop on versions 8.5 and 9. It offers complete coverage of timesaving new features such as the Q

  18. High-energy proton irradiation of C57Bl6 mice under hindlimb unloading

    Science.gov (United States)

    Mendonca, Marc; Todd, Paul; Orschell, Christie; Chin-Sinex, Helen; Farr, Jonathan; Klein, Susan; Sokol, Paul

    2012-07-01

    Solar proton events (SPEs) pose substantial risk for crewmembers on deep space missions. It has been shown that low gravity and ionizing radiation both produce transient anemia and immunodeficiencies. We utilized the C57Bl/6 based hindlimb suspension model to investigate the consequences of hindlimb-unloading induced immune suppression on the sensitivity to whole body irradiation with modulated 208 MeV protons. Eight-week old C57Bl/6 female mice were conditioned by hindlimb-unloading. Serial CBC and hematocrit assays by HEMAVET were accumulated for the hindlimb-unloaded mice and parallel control animals subjected to identical conditions without unloading. One week of hindlimb-unloading resulted in a persistent, statistically significant 10% reduction in RBC count and a persistent, statistically significant 35% drop in lymphocyte count. This inhibition is consistent with published observations of low Earth orbit flown mice and with crewmember blood analyses. In our experiments the cell count suppression was sustained for the entire six-week period of observation and persisted for at least 7 days beyond the period of active hindlimb-unloading. C57Bl/6 mice were also irradiated with 208 MeV Spread Out Bragg Peak (SOBP) protons at the Midwest Proton Radiotherapy Institute at the Indiana University Cyclotron Facility. We found that at 8.5 Gy hindlimb-unloaded mice were significantly more radiation sensitive with 35 lethalities out of 51 mice versus 15 out of 45 control (non-suspended) mice within 30 days of receiving 8.5 Gy of SOBP protons (p =0.001). Both control and hindlimb-unloaded stocktickerCBC analyses of 8.5 Gy proton irradiated and control mice by HEMAVET demonstrated severe reductions in WBC counts (Lymphocytes and PMNs) by day 2 post-irradiation, followed a week to ten days later by reductions in platelets, and then reductions in RBCs about 2 weeks post-irradiation. Recovery of all blood components commenced by three weeks post-irradiation. CBC analyses of 8

  19. 12 CFR 1780.9 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Filing of papers. 1780.9 Section 1780.9 Banks... papers. (a) Filing. Any papers required to be filed shall be addressed to the presiding officer and filed... Director or the presiding officer. All papers filed by electronic media shall also concurrently be filed in...

  20. 77 FR 35371 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-06-13

    .... Applicants: Duke Energy Miami Fort, LLC. Description: MBR Filing to be effective 10/1/2012. Filed Date: 6/5...-000. Applicants: Duke Energy Piketon, LLC. Description: MBR Filing to be effective 10/1/2012. Filed...-1959-000. Applicants: Duke Energy Stuart, LLC. Description: MBR Filing to be effective 10/1/2012. Filed...

  1. 76 FR 63291 - Combined Notice Of Filings #1

    Science.gov (United States)

    2011-10-12

    ... filing per 35: MBR Tariff to be effective 9/23/2011. Filed Date: 09/23/2011. Accession Number: 20110923.... submits tariff filing per 35: MBR Tariff to be effective 9/23/2011. Filed Date: 09/23/2011. Accession.... submits tariff filing per 35: MBR Tariff to be effective 9/23/2011. Filed Date: 09/23/2011. Accession...

  2. Protecting your files on the DFS file system

    CERN Multimedia

    Computer Security Team

    2011-01-01

    The Windows Distributed File System (DFS) hosts user directories for all NICE users plus many more data.    Files can be accessed from anywhere, via a dedicated web portal (http://cern.ch/dfs). Due to the ease of access to DFS with in CERN it is of utmost importance to properly protect access to sensitive data. As the use of DFS access control mechanisms is not obvious to all users, passwords, certificates or sensitive files might get exposed. At least this happened in past to the Andrews File System (AFS) - the Linux equivalent to DFS) - and led to bad publicity due to a journalist accessing supposedly "private" AFS folders (SonntagsZeitung 2009/11/08). This problem does not only affect the individual user but also has a bad impact on CERN's reputation when it comes to IT security. Therefore, all departments and LHC experiments agreed recently to apply more stringent protections to all DFS user folders. The goal of this data protection policy is to assist users in pro...

  3. Protecting your files on the AFS file system

    CERN Multimedia

    2011-01-01

    The Andrew File System is a world-wide distributed file system linking hundreds of universities and organizations, including CERN. Files can be accessed from anywhere, via dedicated AFS client programs or via web interfaces that export the file contents on the web. Due to the ease of access to AFS it is of utmost importance to properly protect access to sensitive data in AFS. As the use of AFS access control mechanisms is not obvious to all users, passwords, private SSH keys or certificates have been exposed in the past. In one specific instance, this also led to bad publicity due to a journalist accessing supposedly "private" AFS folders (SonntagsZeitung 2009/11/08). This problem does not only affect the individual user but also has a bad impact on CERN's reputation when it comes to IT security. Therefore, all departments and LHC experiments agreed in April 2010 to apply more stringent folder protections to all AFS user folders. The goal of this data protection policy is to assist users in...

  4. PHOTOMETRIC STUDIES OF THREE NEGLECTED SHORT-PERIOD CONTACT BINARIES GN BOOTIS, BL LEONIS, AND V1918 CYGNI

    International Nuclear Information System (INIS)

    Yang, Y.-G.; Dai, H.-F.; Qian, S.-B.

    2013-01-01

    We present new photometry for three short-period contact binaries, GN Boo, BL Leo, and V1918 Cyg, observed from 2008 December to 2012 April using several small telescopes in China. Photometric models were deduced from new observations using the updated Wilson-Devinney Code. The results show that GN Boo and BL Leo are W-type contact binaries, while V1918 Cyg is an A-type one. The mass ratios and fill-out factors are q = 0.320(± 0.002) and f = 5.8(± 0.1)% for GN Boo, q = 0.476(± 0.005) and f = 21.3(± 1.1)% for BL Leo, q = 0.264(± 0.002), and f = 49.7(± 0.7)% for V1918 Cyg, respectively. From the (O – C) curves, it is discovered that the orbital periods of three binaries have varied in a complicated way, i.e., cyclic oscillation for GN Boo, long-term period decrease for BL Leo, and both for V1918 Cyg. The cyclic variations for GN Boo and V1918 Cyg may probably be attributed to the magnetic activity of the primary component or light-time effect due to the third body. Meanwhile, the secular period decreases for BL Leo and V1918 Cyg may result from mass transfer from the primary to the secondary, accompanying the mass and angular momentum loss from the central system. Finally, GN Boo, BL Leo, and V1918 Cyg will evolve into deep contact binaries. Additionally, a statistical study of 37 contact binaries with decreasing periods is given. We obtained the relations of q – f and q – dln P/dt, and preliminarily determined the mass loss rate of dln M/dt from the binary system.

  5. 77 FR 60418 - Combined Notice of Filings

    Science.gov (United States)

    2012-10-03

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings Take notice that the Commission has received the following Natural Gas Pipeline Rate and Refund Report filings...-1064-000. Applicants: Venice Gathering System, L.L.C. Description: NAESB 2.0 Compliance Filing to be...

  6. 77 FR 27221 - Combined Notice of Filings

    Science.gov (United States)

    2012-05-09

    ... Generator Status of Minonk Wind, LLC. Filed Date: 4/19/12. Accession Number: 20120419-5196. Comments Due: 5... Self-Certification of Exempt Wholesale Generator Status of Senate Wind, LLC. Filed Date: 4/19/12... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings Take notice...

  7. 77 FR 23474 - Combined Notice of Filings

    Science.gov (United States)

    2012-04-19

    ...: Young Gas Storage Company, Ltd. Description: EBB Notice Categories to be effective 5/15/2012. Filed Date... intervention is necessary to become a party to the proceeding. Filings in Existing Proceedings Docket Numbers... requirements, interventions, protests, and service can be found at: http://www.ferc.gov/docs-filing/efiling...

  8. 76 FR 80921 - Combined Notice of Filings

    Science.gov (United States)

    2011-12-27

    .... Comments Due: 5 p.m. ET 12/27/11. Docket Numbers: RP12-243-000. Applicants: Young Gas Storage Company, Ltd... considered, but intervention is necessary to become a party to the proceeding. The filings are accessible in... can be found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call...

  9. 78 FR 13050 - Combined Notice of Filings

    Science.gov (United States)

    2013-02-26

    ... be considered, but intervention is necessary to become a party to the proceeding. Filings in Existing Proceedings Docket Numbers: RP13-106-002. Applicants: Young Gas Storage Company, Ltd. Description: Young NAESB..., protests, and service can be found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For other...

  10. Site Characterization Data from the U3ax/bl Exploratory Boreholes at the Nevada Test Site

    International Nuclear Information System (INIS)

    2005-01-01

    This report provides qualitative analyses and preliminary interpretations of hydrogeologic data obtained from two 45-degree, slanted exploratory boreholes drilled within the Area 3 Radioactive Waste Management Site (RWMS) at the Nevada Test Site. Borehole UE-3bl-D1 was drilled beneath the U3ax/bl mixed waste disposal unit, and Borehole UE-3bl-U1 was drilled in undisturbed alluvium adjacent to the disposal unit. The U3ax/bl disposal unit is located within two conjoined subsidence craters, U3ax and U3bl, which were created by underground nuclear testing. Data from these boreholes were collected to support site characterization activities for the U3ax/bl disposal unit and the entire Area 3 RWMS. Site characterization at disposal units within the Area 3 RWMS must address the possibility that subsidence craters and associated disturbed alluvium of the chimneys beneath the craters might serve as pathways for contaminant migration. The two boreholes were drilled and sampled to compare hydrogeologic properties of alluvium below the waste disposal unit with those of adjacent undisturbed alluvium. Whether Borehole UE-3bl-D1 actually penetrated the chimney of the U3bl crater is uncertain. Analyses of core samples showed little difference in hydrogeologic properties between the two boreholes. Important findings of this study include the following: No hazardous or radioactive constituents of waste disposal concern were found in the samples obtained from either borehole. No significant differences in physical and hydrogeologic properties between boreholes is evident, and no evidence of significant trends with depth for any of these properties was observed. The values observed are typical of sandy materials. The alluvium is dry, with volumetric water content ranging from 5.6 to 16.2 percent. Both boreholes exhibit a slight increase in water content with depth, the only such trend observed. Water potential measurements on core samples from both boreholes show a large positive

  11. Site Characterization Data from the U3ax/bl Exploratory Boreholes at the Nevada Test Site

    Energy Technology Data Exchange (ETDEWEB)

    Bechtel Nevada; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2005-08-01

    This report provides qualitative analyses and preliminary interpretations of hydrogeologic data obtained from two 45-degree, slanted exploratory boreholes drilled within the Area 3 Radioactive Waste Management Site (RWMS) at the Nevada Test Site. Borehole UE-3bl-D1 was drilled beneath the U3ax/bl mixed waste disposal unit, and Borehole UE-3bl-U1 was drilled in undisturbed alluvium adjacent to the disposal unit. The U3ax/bl disposal unit is located within two conjoined subsidence craters, U3ax and U3bl, which were created by underground nuclear testing. Data from these boreholes were collected to support site characterization activities for the U3ax/bl disposal unit and the entire Area 3 RWMS. Site characterization at disposal units within the Area 3 RWMS must address the possibility that subsidence craters and associated disturbed alluvium of the chimneys beneath the craters might serve as pathways for contaminant migration. The two boreholes were drilled and sampled to compare hydrogeologic properties of alluvium below the waste disposal unit with those of adjacent undisturbed alluvium. Whether Borehole UE-3bl-D1 actually penetrated the chimney of the U3bl crater is uncertain. Analyses of core samples showed little difference in hydrogeologic properties between the two boreholes. Important findings of this study include the following: No hazardous or radioactive constituents of waste disposal concern were found in the samples obtained from either borehole. No significant differences in physical and hydrogeologic properties between boreholes is evident, and no evidence of significant trends with depth for any of these properties was observed. The values observed are typical of sandy materials. The alluvium is dry, with volumetric water content ranging from 5.6 to 16.2 percent. Both boreholes exhibit a slight increase in water content with depth, the only such trend observed. Water potential measurements on core samples from both boreholes show a large positive

  12. 33 CFR 148.246 - When is a document considered filed and where should I file it?

    Science.gov (United States)

    2010-07-01

    ... filed and where should I file it? 148.246 Section 148.246 Navigation and Navigable Waters COAST GUARD... Formal Hearings § 148.246 When is a document considered filed and where should I file it? (a) If a document to be filed is submitted by mail, it is considered filed on the date it is postmarked. If a...

  13. The UV spectrum of the BL Lac object PKS 0521-36

    International Nuclear Information System (INIS)

    Danziger, I.J.; Bergeron, J.; Maraschi, L.; Treves, A.; Milan Univ.; Tanzi, E.G.

    1983-01-01

    Ultraviolet observations (1200 to 3000 A) with the IUE satellite of the BL Lac object PKS 0521-36 are presented. The only emission line which appears clearly in the spectrum is Lyα, which is asymmetric with a component displaced approx. 3000 km s - 1 to the red. The intensity of the line and the upper limits on other lines are compared with the model calculations on QSOs and Seyfert nuclei by Kwan and Krolik. The continuous energy distribution is discussed, combining non-simultaneous observations from the ultraviolet to the infrared. The spectral range of the non-thermal source from far IR to far UV can be described by a single power law of index -1.5. (author)

  14. UV spectrum of the BL Lac object PKS 0521-36

    Energy Technology Data Exchange (ETDEWEB)

    Danziger, I.J. (European Southern Observatory, Garching (Germany, F.R.)); Bergeron, J. (Centre National de la Recherche Scientifique, 75 - Paris (France). Inst. d' Astrophysique); Fosbury, R.A.E. (Royal Greenwich Observatory, Hailsham (UK)); Maraschi, L.; Treves, A. (Consiglio Nazionale delle Ricerche, Milan (Italy). Lab. di Fisica Cosmica; Milan Univ. (Italy). Ist. di Fisica); Tanzi, E.G. (Consiglio Nazionale delle Ricerche, Milan (Italy). Lab. di Fisica Cosmica)

    1983-05-01

    Ultraviolet observations (1200 to 3000 A) with the IUE satellite of the BL Lac object PKS 0521-36 are presented. The only emission line which appears clearly in the spectrum is Ly..cap alpha.., which is asymmetric with a component displaced approx. 3000 km s/sup -1/ to the red. The intensity of the line and the upper limits on other lines are compared with the model calculations on QSOs and Seyfert nuclei by Kwan and Krolik. The continuous energy distribution is discussed, combining non-simultaneous observations from the ultraviolet to the infrared. The spectral range of the non-thermal source from far IR to far UV can be described by a single power law of index -1.5.

  15. Rare codons effect on expression of recombinant gene cassette in Escherichia coli BL21(DE3

    Directory of Open Access Journals (Sweden)

    Aghil Esmaeili-Bandboni

    2017-11-01

    Full Text Available Objective: To demonstrate the sensitivity of expression of fusion genes to existence of a large number of rare codons in recombinant gene sequenced. Methods: Primers for amplification of cholera toxin B, Shiga toxin B and gfp genes were designed by Primer3 software and synthesized. All of these 3 genes were cloned. Then the genes were fused together by restriction sites and enzymatic method. Two linkers were used as a flexible bridge in connection of these genes. Results: Cloning and fusion of cholera toxin B, Shiga toxin B and gfp genes were done correctly. After that, expression of the recombinant gene construction was surveyed. Conclusions: According to what was seen, because of the accumulation of 12 rare codons of Shiga toxin B and 19 rare codons of cholera toxin B in this gene cassette, the expression of the recombinant gene cassette, in Escherichia coli BL21, failed.

  16. Commissioning and first results of scanning type EXAFS beamline (BL-09) at INDUS-2 synchrotron source

    Energy Technology Data Exchange (ETDEWEB)

    Poswal, A. K., E-mail: poswalashwini@gmail.com; Agrawal, A., E-mail: poswalashwini@gmail.com; Yadav, A. K., E-mail: poswalashwini@gmail.com; Nayak, C., E-mail: poswalashwini@gmail.com; Basu, S., E-mail: poswalashwini@gmail.com; Bhattachryya, D.; Jha, S. N.; Sahoo, N. K. [Atomic and Molecular Physics Division, Bhabha Atomic Research Centre, Mumbai -400085 (India); Kane, S. R.; Garg, C. K. [Indus Synchrotrons Utilization Division, Raja Ramanna Centre for Advanced Technology, Indore- 452013 (India)

    2014-04-24

    An Energy Scanning X-ray Absorption Fine Structure spectroscopy beamline has recently been installed and commissioned at BL-09 bending magnet port of INDUS-2 synchrotron source, Indore. The beamline uses an UHV compatible fixed exit double crystal monochromator (DCM) with two Si (111) crystals. Two grazing incidence cylindrical mirrors are also used in this beamline; the pre-mirror is used as a collimating mirror while the post mirror is used for vertical focusing and higher harmonic rejection. In this beamline it is possible to carry out EXAFS measurements both in transmission and fluorescence mode on various types of samples, using Ionization chamber detectors and solid state drift detector respectively. In this paper, results from first experiments of the Energy Scanning EXAFS beamline are presented.

  17. Toward operation of series IDs at BL43LXU of SPring-8

    Energy Technology Data Exchange (ETDEWEB)

    Baron, A. Q. R.; Tanaka, T.; Soutome, K.; Takao, M.; Nakamura, T.; Kobayashi, K.; Fujita, T.; Takahashi, S.; Aoyagi, H.; Shimosaki, Y.; Seike, T.; Uchiyama, H.; Ishikawa, D.; Chuang, T.-H.; Kimura, H.; Tanaka, H.; Kitamura, H.; Ishikawa, T. [SPring-8, 1-1-1 Kouto, Sayo, Hyogo, 679 (Japan)

    2016-07-27

    This paper discusses two issues relating to using 3 small gap insertion devices in series at BL43LXU of SPring-8 to make a uniquely powerful source in the 15-26 keV region of the x-ray spectrum. The issues discussed are (1) damage to the covers of the downstream IDs by radiation from the upstream IDs and (2) proper steering of the electron beam to get the best photon beam properties. After tests in several configurations, including one where an ID was run without an impedance-reducing cover, the damage issue was solved by installing a distributed absorber in the most downstream ID. The steering issues were mostly resolved by the introduction of appropriate corrector magnets and feedback. The paper is written from the viewpoint of an interested beamline scientist impressed with the cooperation of different groups to make a source for new science possible.

  18. Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice.

    Science.gov (United States)

    Huang, Qiling; Ma, Xiaomeng; Zhu, Dong Liang; Chen, Li; Jiang, Ying; Zhou, Linli; Cen, Lei; Pi, Rongbiao; Chen, Xiaohong

    2015-07-15

    Total glucosides of peony (TGP), an active compound extracted from the roots of Paeonia lactiflora Pall, has wide pharmacological effects on nervous system. Here we examined the effects of TGP on experimental autoimmune encephalomyelitis (EAE), an established model of multiple sclerosis (MS). The results showed that TGP can reduce the severity and progression of EAE in C57 BL/6 mice. In addition, TGP also down-regulated the Th1/Th17 inflammatory response and prevented the reduced expression of brain-derived neurotrophic factor and 2',3'-cyclic nucleotide 3'-phosphodiesterase of EAE. These findings suggest that TGP could be a potential therapeutic agent for MS. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Newly designed double surface bimorph mirror for BL-15A of the photon factory

    Energy Technology Data Exchange (ETDEWEB)

    Igarashi, Noriyuki, E-mail: noriyuki.igarashi@kek.jp; Nitani, Hiroaki; Takeichi, Yasuo; Niwa, Yasuhiro; Abe, Hitoshi; Kimura, Masao; Mori, Takeharu; Nagatani, Yasuko; Kosuge, Takashi; Kamijo, Ai; Koyama, Atsushi; Shimizu, Nobutaka [Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization 1-1 Oho, Tsukuba, Ibaraki 305-0801 (Japan); Ohta, Hiromasa [Mitsubishi Electric System & Service Co., Ltd. 20F Carrot Tower 4-1-1 Taishido, Setagaya-ku, Tokyo 154-8520 (Japan)

    2016-07-27

    BL-15A is a new x-ray undulator beamline at the Photon Factory. It will be dedicated to two independent research activities, simultaneous XAFS/XRF/XRD experiments, and SAXS/WAXS/GI-SAXS studies. In order to supply a choice of micro-focus, low-divergence and collimated beams, a double surface bimorph mirror was recently developed. To achieve further mirror surface optimization, the pencil beam scanning method was applied for “in-situ” beam inspection and the Inverse Matrix method was used for determination of optimal voltages on the piezoelectric actuators. The corrected beam profiles at every focal spot gave good agreement with the theoretical values and the resultant beam performance is promising for both techniques. Quick and stable switching between highly focused and intense collimated beams was established using this new mirror with the simple motorized stages.

  20. The bio-crystallography beamline (BL41XU) at SPring-8

    CERN Document Server

    Kawamoto, M; Kamiya, N

    2001-01-01

    The bio-crystallography beamline (BL41XU), one of two pilot beamlines at SPring-8, was constructed using a standard in-vacuum-type undulator and opened for general users from domestic and overseas countries. Many tests and improvements were carried out on beamline elements and equipment for macromolecular crystallography, especially on the so-called 'pin-post' water cooling crystal of rotated-inclined double crystal monochromator. The maximum brilliance at sample position reached to 4x10 sup 1 sup 5 photons/s/mm sup 2 /mrad sup 2 at an X-ray energy of 11 keV. Commercially available X-ray detectors of CCD and imaging plate were installed in the experimental station. A beamline control software system for beam tracking and an on-line reader for large-format imaging plate were newly developed.

  1. Hydrolyzed Casein Reduces Diet-Induced Obesity in Male C57BL/6J Mice

    DEFF Research Database (Denmark)

    Lillefosse, Haldis H.; Tastesen, Hanne Sørup; Du, Zhen-Yu

    2013-01-01

    used a factorial ANOVA design to investigate the effects of protein form (intact vs. hydrolyzed casein) and protein level (16 vs. 32 energy percent protein) on body mass gain and adiposity in obesity-prone male C57BL/6J mice fed Western diets with 35 energy percent fat. Mice fed the hydrolyzed casein......The digestion rate of dietary protein is a regulating factor for postprandial metabolism both in humans and animal models. However, few data exist about the habitual consumption of proteins with different digestion rates with regard to the development of body mass and diet-induced obesity. Here, we...... diets had higher spontaneous locomotor activity than mice fed intact casein. During the light phase, mice fed hydrolyzed casein tended (P = 0.08) to have a lower respiratory exchange ratio, indicating lower utilization of carbohydrates as energy substrate relative to those fed intact casein. In further...

  2. Soft x-ray beamline BL7A at the UVSOR

    International Nuclear Information System (INIS)

    Murata, T.; Matsukawa, T.; Naoe, S.; Horigome, T.; Matsudo, O.; Watanabe, M.

    1992-01-01

    A vacuum-compatible double-crystal monochromator with constant exit-beam height has been installed and operated for several years at beamline BL7A at the UVSOR facility at the Institute for Molecular Science, Okazaki, Japan, The beamline is used both for the photons from a normal bending-magnet section and those from a 4 T superconducting three-pole horizontal wiggler. Various pairs of monochromator crystals are being used with sufficient intensity and signal to noise ratio of the output signal. Basic structure of the beamline, the mechanism of the monochromator, and some typical spectra of materials with absorption edges between 850 eV and 4 keV are reported

  3. [Presbycusis: neural degeneration and aging on the auditory receptor of C57/BL6J mice].

    Science.gov (United States)

    Castillo, E; Carricondo, F; Bartolomé, M V; Vicente-Torres, A; Poch Broto, J; Gil-Loyzaga, P

    2006-11-01

    Presbycusis is a progressive hearing impairment associated with aging, characterized by hearing loss and a degeneration of cochlear structures. In this paper we analyze the effects of aging on the auditory system of C57/BL6J mice, with electrophysiological and morphological studies. With this aim the auditory potentials of mice aging 1, 3, 6, 9, 12, 15, 18, 21 and 24 months were recorded, and then the morphology of the cochleal were analyzed. Auditory potentials revealed an increase in wave latencies, as well as a decrease in their amplitudes during aging. Morphological results showed a total Corti's organ degeneration, being replaced by a flat epithelial layer, and a total absence of hair cells.

  4. Antioxidant and free radical scavenging activities of Gastrodia elata Bl. and Uncaria rhynchophylla (Miq.) Jacks.

    Science.gov (United States)

    Liu, J; Mori, A

    1992-12-01

    Gastrodia elata Bl. (GE) and Uncaria rhynchophylla (Miq.) Jacks (UR) are two traditional Chinese medicinal herbal drugs, used for the treatment of convulsions and epilepsy. Their antioxidant effects in vivo and their free radical scavenging effects in vitro were investigated. Epileptogenic foci in the lateral brain of the rat were induced by the injection of ferric chloride into the lateral cortex. Both extracts significantly inhibited the increase in levels of lipid peroxide in the ipsilateral cortex, at all times observed. In addition, the two extracts also induced an early increase of activity of superoxide dismutase in the mitochondrial fraction of the ipsilateral cortex. In in vitro experiments, the two extracts exhibited significant dose-dependent scavenging effects on free radicals, using electron spin resonance spectroscopy. These results suggest that the proposed antiepileptic effects of GE and UR may be attributable to the antioxidant activity of the active components in these two medicinal herbs.

  5. Spectroscopy of 10 γ -Ray BL Lac Objects at High Redshift

    Energy Technology Data Exchange (ETDEWEB)

    Paiano, Simona; Falomo, Renato [INAF, Osservatorio Astronomico di Padova, Vicolo dell’Osservatorio 5, I-35122 Padova (Italy); Landoni, Marco [INAF, Osservatorio Astronomico di Brera, Via E. Bianchi 46, I-23807 Merate (Italy); Treves, Aldo [Università degli Studi dell’Insubria, Via Valleggio 11, I-22100 Como (Italy); Scarpa, Riccardo [Instituto de Astrofisica de Canarias, C/O Via Lactea, s/n E-38205 La Laguna, Tenerife (Spain)

    2017-08-01

    We present optical spectra with high signal-to-noise ratio of 10 BL Lac objects detected at GeV energies by the Fermi satellite (3FGL catalog), which previous observations suggested are at relatively high redshift. The new observations, obtained at the 10 m Gran Telescopio Canarias, allowed us to find the redshift for J0814.5+2943 ( z = 0.703), and we can set a spectroscopic lower limit for J0008.0+4713 ( z > 1.659) and J1107.7+0222 ( z > 1.0735) on the basis of Mg ii intervening absorption features. In addition we confirm the redshifts for J0505.5+0416 ( z = 0.423) and J1450+5200 ( z > 2.470). Finally we contradict the previous z estimates for five objects (J0049.7+0237, J0243.5+7119, J0802.0+1005, J1109.4+2411, and J2116.1+3339).

  6. The effect of cage size on reproductive performance and behavior of C57BL/6 mice.

    Science.gov (United States)

    Whitaker, Julia; Moy, Sheryl S; Saville, Benjamin R; Godfrey, Virginia; Nielsen, Judith; Bellinger, Dwight; Bradfield, John

    2007-11-01

    Scientific research has yet to conclusively determine the optimal cage size for mice. The authors examined the effect of cage size on mouse breeding performance and on offspring behavior, which can serve as indications of overall well-being. They housed breeding trios of C57BL/6Tac mice in standard or large individually ventilated cages and measured four reproductive parameters: litter size; litter survival to weaning age; average pup weight at 7, 14 and 21 days; and the number of days between litter births. They investigated the behavior of a subset of male and female pups from parents housed in cages of each size in the elevated plus maze test, the open field assay and the acoustic startle test. Cage size had no significant effect on any of the reproductive parameters measured and few or inconsistent effects on behavior in weaned pups.

  7. Magnetic Field Studies in BL Lacertae through Faraday Rotation and a Novel Astrometric Technique

    Directory of Open Access Journals (Sweden)

    Sol N. Molina

    2017-12-01

    Full Text Available It is thought that dynamically important helical magnetic fields twisted by the differential rotation of the black hole’s accretion disk or ergosphere play an important role in the launching, acceleration, and collimation of active galactic nuclei (AGN jets. We present multi-frequency astrometric and polarimetric Very Long Baseline Array (VLBA images at 15, 22, and 43 GHz, as well as Faraday rotation analyses of the jet in BL Lacertae as part of a sample of AGN jets aimed to probe the magnetic field structure at the innermost scales to test jet formation models. The novel astrometric technique applied allows us to obtain the absolute position at mm wavelengths without any external calibrator.

  8. Bisphenol A impairs hepatic glucose sensing in C57BL/6 male mice.

    Directory of Open Access Journals (Sweden)

    Leigh Perreault

    Full Text Available AIMS/HYPOTHESIS: Glucose sensing (eg. glucokinase activity becomes impaired in the development of type 2 diabetes, the etiology of which is unclear. Estrogen can stimulate glucokinase activity, whereas the pervasive environmental pollutant bisphenol A (BPA can inhibit estrogen action, hence we aimed to determine the effect of BPA on glucokinase activity directly. METHODS: To evaluate a potential acute effect on hepatic glucokinase activity, BPA in water (n = 5 vs. water alone (n = 5 was administered at the EPA's purported "safe dose" (50 µg/kg by gavage to lean 6-month old male C57BL/6 mice. Two hours later, animals were euthanized and hepatic glucokinase activity measured over glucose levels from 1-20 mmol/l in liver homogenate. To determine the effect of chronic BPA exposure on hepatic glucokinase activity, lean 6-month old male C57BL/6 mice were provided with water (n = 15 or water with 1.75 mM BPA (∼50 µg/kg/day; n = 14 for 2 weeks. Following the 2-week exposure, animals were euthanized and glucokinase activity measured as above. RESULTS: Hepatic glucokinase activity was signficantly suppressed after 2 hours in animals given an oral BPA bolus compared to those who received only water (p = 0.002-0.029 at glucose 5-20 mmol/l; overall treatment effect p<0.001. Exposure to BPA over 2 weeks also suppressed hepatic glucokinase activity in exposed vs. unexposed mice (overall treatment effect, p = 0.003. In both experiments, the Hill coefficient was higher and Vmax lower in mice treated with BPA. CONCLUSIONS/INTERPRETATION: Both acute and chronic exposure to BPA significantly impair hepatic glucokinase activity and function. These findings identify a potential mechanism for how BPA may increase risk for diabetes.

  9. Attentional processing in C57BL/6J mice exposed to developmental vitamin D deficiency.

    Directory of Open Access Journals (Sweden)

    Lauren R Harms

    Full Text Available Epidemiological evidence suggests that Developmental Vitamin D (DVD deficiency is associated with an increased risk of schizophrenia. DVD deficiency in mice is associated with altered behaviour, however there has been no detailed investigation of cognitive behaviours in DVD-deficient mice. The aim of this study was to determine the effect of DVD deficiency on a range of cognitive tasks assessing attentional processing in C57BL/6J mice. DVD deficiency was established by feeding female C57BL/6J mice a vitamin D-deficient diet from four weeks of age. After six weeks on the diet, vitamin D-deficient and control females were mated with vitamin D-normal males and upon birth of the pups, all dams were returned to a diet containing vitamin D. The adult offspring were tested on a range of cognitive behavioural tests, including the five-choice serial reaction task (5C-SRT and five-choice continuous performance test (5C-CPT, as well as latent inhibition using a fear conditioning paradigm. DVD deficiency was not associated with altered attentional performance on the 5C-SRT. In the 5C-CPT DVD-deficient male mice exhibited an impairment in inhibiting repetitive responses by making more perseverative responses, with no changes in premature or false alarm responding. DVD deficiency did not affect the acquisition or retention of cued fear conditioning, nor did it affect the expression of latent inhibition using a fear conditioning paradigm. DVD-deficient mice exhibited no major impairments in any of the cognitive domains tested. However, impairments in perseverative responding in DVD-deficient mice may indicate that these animals have specific alterations in systems governing compulsive or reward-seeking behaviour.

  10. Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice

    Directory of Open Access Journals (Sweden)

    Seon-Yong Lee

    2016-06-01

    Full Text Available Objectives: Cornu cervi pantotrichum (CCP has been widely used in Korean and China, as an anti-fatigue, anti-aging, and tonic agent to enhance the functions of the reproductive and the immune systems. Because CCP has various growth factors that play important roles in the development of hair follicles, we examined whether CCP pharmacopuncture solution (CCPPS was capable of promoting hair growth in an animal model. Methods: One day after hair depilation, CCPPS were topically applied to the dorsal skin of C57BL/6 mice once a day for 15 days. Hair growth activity was evaluated by using macro- and microscopic observations. Dorsal skin tissues were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU, proliferating cell nuclear antigen (PCNA, and fibroblast growth factor (FGF-7 were examined by using immunohistochemical staining. A reverse transcription polymerase chain reaction (RT-PCR analysis was also conducted to measure the messenger RNA (mRNA expression of FGF-7. Results: CCPPS induced more active hair growth than normal saline. Histologic analysis showed enlargement of the dermal papilla, elongation of the hair shaft, and expansion of hair thickness in CCPPS treated mice, indicating that CCPPS effectively induced the development of anagen. CCPPS treatment markedly increased the expressions of BrdU and PCNA in the hair follicles of C57BL/6 mice. In addition, CCPPS up regulated the expression of FGF-7, which plays an important role in the development of hair follicles. Conclusion: These results reveal that CCPPS facilitates hair re-growth by proliferation of hair follicular cells and up-regulation of FGF-7 and suggest that CCPPS can potentially be applied as an alternative treatment for patients with alopecia.

  11. The NuSTAR view on Hard-TeV BL Lacs

    Science.gov (United States)

    Costamante, L.; Bonnoli, G.; Tavecchio, F.; Ghisellini, G.; Tagliaferri, G.; Khangulyan, D.

    2018-05-01

    Hard-TeV BL Lacs are a new type of blazars characterized by a hard intrinsic TeV spectrum, locating the peak of their gamma-ray emission in the spectral energy distribution (SED) above 2-10 TeV. Such high energies are problematic for the Compton emission, using a standard one-zone leptonic model. We study six examples of this new type of BL Lacs in the hard X-ray band with NuSTAR. Together with simultaneous observations with the Neil Gehrels Swift Observatory, we fully constrain the peak of the synchrotron emission in their SED, and test the leptonic synchrotron self-Compton (SSC) model. We confirm the extreme nature of 5 objects also in the synchrotron emission. We do not find evidence of additional emission components in the hard X-ray band. We find that a one-zone SSC model can in principle reproduce the extreme properties of both peaks in the SED, from X-ray up to TeV energies, but at the cost of i) extreme electron energies with very low radiative efficiency, ii) conditions heavily out of equipartition (by 3 to 5 orders of magnitude), and iii) not accounting for the simultaneous UV data, which then should belong to a different emission component, possibly the same as the far-IR (WISE) data. We find evidence of this separation of the UV and X-ray emission in at least two objects. In any case, the TeV electrons must not "see" the UV or lower-energy photons, even if coming from different zones/populations, or the increased radiative cooling would steepen the VHE spectrum.

  12. Oxycodone physical dependence and its oral self-administration in C57BL/6J mice.

    Science.gov (United States)

    Enga, Rachel M; Jackson, Asti; Damaj, M Imad; Beardsley, Patrick M

    2016-10-15

    Abuse of prescription opioids, such as oxycodone, has markedly increased in recent decades. While oxycodone's antinociceptive effects have been detailed in several preclinical reports, surprisingly few preclinical reports have elaborated its abuse-related effects. This is particularly surprising given that oxycodone has been in clinical use since 1917. In a novel oral operant self-administration procedure, C57BL/6J mice were trained to self-administer water before introducing increasing concentrations of oxycodone (0.056-1.0mg/ml) under post-prandial conditions during daily, 3-h test sessions. As the concentration of oxycodone increased, the numbers of deliveries first increased, then decreased in an inverted U-shape fashion characteristic of the patterns of other drugs self-administered during limited access conditions. After post-prandial conditions were removed, self-administration at the highest concentration was maintained suggesting oral oxycodone served as a positive reinforcer. In other mice, using a novel regimen of physical dependence, mice were administered increasing doses of oxycodone (9.0-33.0mg/kg, s.c.) over 9 days, challenged with naloxone (0.1-10.0mg/kg, s.c.), and then observed for 30min. Naloxone dose-dependently increased the observed number of somatic signs of withdrawal, suggesting physical dependence of oxycodone was induced under this regimen. This is the first report demonstrating induction of oral operant self-administration of oxycodone and dose-dependent precipitations of oxycodone withdrawal in C57BL/6J mice. The use of oral operant self-administration as well as the novel physical dependence regimen provides useful approaches to further examine the abuse- and dependence-related effects of this highly abused prescription opioid. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Effect of fenbendazole on three behavioral tests in male C57BL/6N mice.

    Science.gov (United States)

    Gadad, Bharathi S; Daher, João P L; Hutchinson, Eric K; Brayton, Cory F; Dawson, Ted M; Pletnikov, Mikhail V; Watson, Julie

    2010-11-01

    Pinworms are highly contagious parasites of laboratory rodents that often are treated with fenbendazole. To our knowledge, the effect of fenbendazole at therapeutic dosages on behavioral tests in mice has not been evaluated. Here we studied 6-wk-old male C57BL/6N mice. We compared the behavior of control mice (fed regular diet) with 3 groups of mice treated with dietary fenbendazole. Treatment groups were 4 wk of fenbendazole, 2 wk of fenbendazole followed by 2 wk of regular diet, and 2 wk of regular diet followed by 2 wk of fenbendazole. At the end of dietary treatment all groups were tested by open field for central, peripheral and vertical activity; elevated plus maze for anxiety; and rotarod for motor ability and then evaluated by clinical pathology and selected histopathology. Treated and control groups showed no differences in open field or elevated plus maze testing, histopathology, or clinical pathology. However mice treated for 4 wk with fenbendazole or 2 wk of fenbendazole followed by 2 wk regular diet stayed on the rotarod for shorter periods than did controls, and mice treated with 2 wk of regular diet followed by 2 wk fenbendazole showed a trend toward shorter rotarod times. In light of this study, we suggest that open field and elevated plus maze testing is unlikely to be affected by 4 wk fenbendazole treatment in male C57BL/6 mice; however, behavioral tests of motor ability such as rotarod tests may be affected during and for at least 2 wk after fenbendazole treatment.

  14. Studies of the jet in BL Lacertae. I. Recollimation shock and moving emission features

    Energy Technology Data Exchange (ETDEWEB)

    Cohen, M. H.; Hovatta, T. [Department of Astronomy, California Institute of Technology, Pasadena, CA 91125 (United States); Meier, D. L. [Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 (United States); Arshakian, T. G. [I. Physikalisches Institut, Universität zu Köln, Zülpicher Strasse 77, D-50937 Köln (Germany); Homan, D. C. [Department of Physics, Denison University, Granville, OH 43023 (United States); Kovalev, Y. Y. [Astro Space Center of Lebedev Physical Institute, Profsoyuznaya 84/32, 117997 Moscow (Russian Federation); Lister, M. L.; Richards, J. L. [Department of Physics and Astronomy, Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907 (United States); Pushkarev, A. B.; Savolainen, T., E-mail: mhc@astro.caltech.edu [Max-Planck-Institut für Radioastronomie, Auf Dem Hügel 69, D-53121 Bonn (Germany)

    2014-06-01

    Parsec-scale VLBA images of BL Lac at 15 GHz show that the jet contains a permanent quasi-stationary emission feature 0.26 mas (0.34 pc projected) from the core, along with numerous moving features. In projection, the tracks of the moving features cluster around an axis at a position angle of –166.°6 that connects the core with the standing feature. The moving features appear to emanate from the standing feature in a manner strikingly similar to the results of numerical two-dimensional relativistic magneto-hydrodynamic (RMHD) simulations in which moving shocks are generated at a recollimation shock (RCS). Because of this, and the close analogy to the jet feature HST-1 in M87, we identify the standing feature in BL Lac as an RCS. We assume that the magnetic field dominates the dynamics in the jet, and that the field is predominantly toroidal. From this we suggest that the moving features are compressions established by slow and fast mode magneto-acoustic MHD waves. We illustrate the situation with a simple model in which the slowest moving feature is a slow-mode wave, and the fastest feature is a fast-mode wave. In the model, the beam has Lorentz factor Γ{sub beam}{sup gal}≈3.5 in the frame of the host galaxy and the fast mode wave has Lorentz factor Γ{sub Fwave}{sup beam}≈1.6 in the frame of the beam. This gives a maximum apparent speed for the moving features, β{sub app} = v{sub app}/c = 10. In this model the Lorentz factor of the pattern in the galaxy frame is approximately three times larger than that of the beam itself.

  15. The Perceptions of “Healing” in BL (Boys’ Love Reading and the Situational Triggers

    Directory of Open Access Journals (Sweden)

    Chieh-Yu Chang

    2015-10-01

    Full Text Available Boys’ Love (BL is a fiction genre now enjoying high visibility and popularity among the young women in Taiwan. Inspired by the reader response theory, this study adopted a grounded theory approach to analyze female readers’ perception of “healing” generated from BL reading. Based on the in-depth interviews with 22 readers, this study revealed three types of “healing” perception and four types of situations that prompted the emergence of the feelings. The three types of “healing” perception included the perceptions induced from the descriptions of interpersonal relations in the stories (i.e., the feeling of sweetness from reading about romantic love, coziness from reading about family love and friendships, and the pain and joy of looking back at the self’s past, perceptions triggered by storylines or story characters (i.e., feeling relieved, feeling soothed, feeling of completion and satisfaction, feeling hilarious, feeling pampering toward story characters, and perceptions triggered by visual or audio sensations. The four types of situations for the emergence of the healing perception included the matching of textual characteristics and readers’ romantic experiences, the matching of textual characteristics and readers’ life experiences, an atmosphere of tenderness and softness induced by textual characteristics, and the reading of derivative creation based on other popular original fiction works that bring about satisfaction and completion. Based on the findings, this paper further discusses on the significance of the healing effects generated from everyday pleasure reading as well as the contribution of the analysis approach used in this study for future genre reading research.

  16. Beyond a Terabyte File System

    Science.gov (United States)

    Powers, Alan K.

    1994-01-01

    The Numerical Aerodynamics Simulation Facility's (NAS) CRAY C916/1024 accesses a "virtual" on-line file system, which is expanding beyond a terabyte of information. This paper will present some options to fine tuning Data Migration Facility (DMF) to stretch the online disk capacity and explore the transitions to newer devices (STK 4490, ER90, RAID).

  17. Remote file inquiry (RFI) system

    Science.gov (United States)

    1975-01-01

    System interrogates and maintains user-definable data files from remote terminals, using English-like, free-form query language easily learned by persons not proficient in computer programming. System operates in asynchronous mode, allowing any number of inquiries within limitation of available core to be active concurrently.

  18. Analyzing data files in SWAN

    CERN Document Server

    Gajam, Niharika

    2016-01-01

    Traditionally analyzing data happens via batch-processing and interactive work on the terminal. The project aims to provide another way of analyzing data files: A cloud-based approach. It aims to make it a productive and interactive environment through the combination of FCC and SWAN software.

  19. Log files for testing usability

    NARCIS (Netherlands)

    Klein Teeselink, G.; Siepe, A.H.M.; Pijper, de J.R.

    1999-01-01

    The aim of this study is to gain insight in the usefulness of log file analysis as a method to evaluate the usability of individual interface components and their influence on the usability of the overall user interface. We selected a music player as application, with four different interfaces and

  20. Nuclear Structure References (NSR) file

    International Nuclear Information System (INIS)

    Ewbank, W.B.

    1978-08-01

    The use of the Nuclear Structure References file by the Nuclear Data Project at ORNL is described. Much of the report concerns format information of interest only to those preparing input to the system or otherwise needing detailed knowledge of its internal structure. 17 figures

  1. Induced immune response of Escherichia coli BL21 expressing recombinant MSP1a and MSP1b proteins of Anaplasma marginale

    Directory of Open Access Journals (Sweden)

    Katia Tamekuni

    2009-11-01

    Full Text Available This work aims to evaluate the potential of immunization with E. coli BL21 expressing the recombinant rMSP1a and rMSP1b proteins of Anaplasma marginale. E. coli BL21 was transformed with recombinant plasmids pET102/msp1α and pET101/msp1β, and rMSP1a and rMSP1b were expressed after induction by IPTG. BALB/c mice were vaccinated with formolized BL21/rMSP1a and BL21/rMSP1b, and the production in mice sera of whole IgG was determined by ELISA. The mice immunized with BL21/rMSP1a showed a better humoral response for whole IgG when compared to the mice immunized with BL21/rMSP1b; these mice exhibited a small response after the second vaccination. Sera of mice immunized with BL21/rMSP1a reacted via western blot with BL21 and rMSP1a, with molecular masses varying from 70 to 105 kDa. Sera of mice immunized with BL21/rMSP1b reacted with BL21 and rMSP1b with a molecular mass of 100 kDa. These results demonstrate that BL21 containing rMSP1a and rMSP1b in the outer membrane were able to produce an immune response in mice, reinforcing its use in vaccine models against bovine anaplasmosis.Esse trabalho avaliou o potencial de imunização de Escherichia coli BL21 expressando as proteínas recombinantes rMSP1a e rMSP1b de Anaplasma marginale. A E. coli BL21 foi transformada com os plasmídios recombinantes pET102/msp1α e pET101/msp1β e as proteínas rMSP1a e rMSP1b foram expressas após indução com IPTG. Camundongos BALB/c foram vacinados com BL21/rMSP1a e BL21/rMSP1b formolisadas, e a produção de IgG total foi determinada pelo teste de ELISA nos soros dos camundongos imunizados. Os camundongos imunizados com a BL21/rMSP1a mostraram uma melhor resposta humoral para IgG total, comparada à resposta apresentada pelos camundongos imunizados com BL21/rMSP1b; estes camundongos exibiram uma menor resposta após a segunda vacinação. Soros de camundongos imunizados BL21/rMSP1a reagiram pelo western blot com BL21 e rMSP1a, com massa molecular variando de 70 a

  2. A Metadata-Rich File System

    Energy Technology Data Exchange (ETDEWEB)

    Ames, S; Gokhale, M B; Maltzahn, C

    2009-01-07

    Despite continual improvements in the performance and reliability of large scale file systems, the management of file system metadata has changed little in the past decade. The mismatch between the size and complexity of large scale data stores and their ability to organize and query their metadata has led to a de facto standard in which raw data is stored in traditional file systems, while related, application-specific metadata is stored in relational databases. This separation of data and metadata requires considerable effort to maintain consistency and can result in complex, slow, and inflexible system operation. To address these problems, we have developed the Quasar File System (QFS), a metadata-rich file system in which files, metadata, and file relationships are all first class objects. In contrast to hierarchical file systems and relational databases, QFS defines a graph data model composed of files and their relationships. QFS includes Quasar, an XPATH-extended query language for searching the file system. Results from our QFS prototype show the effectiveness of this approach. Compared to the defacto standard, the QFS prototype shows superior ingest performance and comparable query performance on user metadata-intensive operations and superior performance on normal file metadata operations.

  3. Deceit: A flexible distributed file system

    Science.gov (United States)

    Siegel, Alex; Birman, Kenneth; Marzullo, Keith

    1989-01-01

    Deceit, a distributed file system (DFS) being developed at Cornell, focuses on flexible file semantics in relation to efficiency, scalability, and reliability. Deceit servers are interchangeable and collectively provide the illusion of a single, large server machine to any clients of the Deceit service. Non-volatile replicas of each file are stored on a subset of the file servers. The user is able to set parameters on a file to achieve different levels of availability, performance, and one-copy serializability. Deceit also supports a file version control mechanism. In contrast with many recent DFS efforts, Deceit can behave like a plain Sun Network File System (NFS) server and can be used by any NFS client without modifying any client software. The current Deceit prototype uses the ISIS Distributed Programming Environment for all communication and process group management, an approach that reduces system complexity and increases system robustness.

  4. Comparison of male chimeric mice generated from microinjection of JM8.N4 embryonic stem cells into C57BL/6J and C57BL/6NTac blastocysts.

    Science.gov (United States)

    Fielder, Thomas J; Yi, Charles S; Masumi, Juliet; Waymire, Katrina G; Chen, Hsiao-Wen; Wang, Shuling; Shi, Kai-Xuan; Wallace, Douglas C; MacGregor, Grant R

    2012-12-01

    To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the same JM8.N4 (C57BL/6NTac) derived ES cell line into blastocysts of mixed sex from either C57BL/6J (B6J) or C57BL/6NTac (B6NTac) mice. Similar efficiency of production and sex-conversion of chimeric animals was observed with each strain of blastocyst. However, B6J chimeric males had fewer developmental abnormalities involving urogenital and reproductive tissues (1/12, 8%) compared with B6NTac chimeric males (7/9, 78%). The low sample size did not permit determination of statistical significance for many parameters. However, in each category analyzed the B6J-derived chimeric males performed as well, or better, than their B6NTac counterparts. Twelve of 14 (86%) B6J male chimeras were fertile compared with 6 of 11 (55%) B6NTac male chimeras. Ten of 12 (83%) B6J chimeric males sired more than 1 litter compared with only 3 of 6 (50%) B6NTac chimeras. B6J male chimeras produced more litters per productive mating (3.42 ± 1.73, n = 12) compared to B6NTac chimeras (2.17 ± 1.33, n = 6). Finally, a greater ratio of germline transmitting chimeric males was obtained using B6J blastocysts (9/14; 64%) compared with chimeras produced using B6NTac blastocysts (4/11; 36%). Use of B6J host blastocysts for microinjection of ES cells may offer improvements over blastocysts from B6NTac and possibly other sub-strains of C57BL/6 mice.

  5. Energy distribution and variability of BL Lac objects. The cases of PKS 2155-304 and 3C 66A

    International Nuclear Information System (INIS)

    Maraschi, L.; Maccagni, D.; Tanzi, E.G.; Treves, A.

    1983-01-01

    The BL Lacertae objects, PKS 2155-304 and 3C66A have been observed in the ultraviolet and the X-ray region. The radiation flux in both regions are discussed with respect to its energy and time dependence. (Auth.)

  6. [Application of locomotor activity test to evaluate functional injury after global cerebral ischemia in C57BL/6 mice].

    Science.gov (United States)

    Zhang, Li-quan; Xu, Jia-ni; Wang, Zhen-zhen; Zeng, Li-jun; Ye, Yi-lu; Zhang, Wei-ping; Wei, Er-qing; Zhang, Qi

    2014-05-01

    To evaluate the application of locomotor activity test in functional injury after global cerebral ischemia (GCI) in C57BL/6 mice. GCI was induced by bilateral carotid arteries occlusion for 30 min in C57BL/6 mice. Mice were divided into sham group, GCI group and minocycline group. Saline or minocycline (45 mg/kg) was i.p. injected once daily for 6 d after ischemia. At Day 6 after ischemia, locomotor activity was recorded for 1 h in open field test. Total distance, central distance, central distance ratio, periphery distance, periphery distance ratio, central time and periphery time were used to evaluate the behavior characteristics of locomotor activity in C57BL/6 mice after ischemia. The survival neuron density was detected by Nissl staining in hippocampus, cortex and striatum. Compared with sham group, total distance, central distance and central time increased and periphery time decreased in C57BL/6 mice after GCI (PsLocomotor activity in open field test can objectively evaluate the behavior injury after GCI in mice. Central distance and central time can be used as indexes of quantitative assessment.

  7. Strawinsky: Ragtime, Oktett für Bläser, Neeme Järvi / Werner Pfister

    Index Scriptorium Estoniae

    Pfister, Werner

    1995-01-01

    Uuest heliplaadist "Strawinsky: Ragtime, Oktett für Bläser, Die Geschichte vom Soldaten (Suite), Petruschka. Royal Scottish National Orchestra, Orchestre de la Suisse Romande, Neeme Järvi". (AD: 1986-93). Chandos/Koch CD 9291 (WD:74'40")

  8. B16-BL6 melanoma cells release inhibitory factor(s) of active pump activity in isolated lymph vessels.

    Science.gov (United States)

    Nakaya, K; Mizuno, R; Ohhashi, T

    2001-12-01

    We investigated whether supernatant cultured with melanoma cell lines B16-BL6 and K1735 or the Lewis lung carcinoma cell line (LLC) can regulate lymphatic pump activity with bioassay preparations isolated from murine iliac lymph vessels. B16-BL6 and LLC supernatants caused significant dilation of lymph microvessels with cessation of pump activity. B16-BL6 supernatant produced dose-related cessation of lymphatic pump activity. There was no significant tachyphylaxis in the supernatant-mediated inhibitory response of lymphatic pump activity. Pretreatment with 3 x 10(-5) M N(omega)-nitro-L-arginine methyl ester (L-NAME) or 10(-7) M or 10(-6) M glibenclamide and 5 x 10(-4) M 5-hydroxydecanoic acid caused significant reduction of supernatant-mediated inhibitory responses. Simultaneous treatment with 10(-3) M L-arginine and 3 x 10(-5) M L-NAME significantly lessened L-NAME-induced inhibition of the supernatant-mediated response, suggesting that endogenous nitric oxide (NO) plays important roles in supernatant-mediated inhibitory responses. Chemical treatment dialyzed substances of B16-BL6 cells may release nonpeptide substance(s) of <1,000 MW, resulting in significant cessation of lymphatic pump activity via production and release of endogenous NO and activation of mitochondrial ATP-sensitive K(+) channels.

  9. Dietary magnesium deficiency affects gut microbiota and anxiety-like behaviour in C57BL/6N mice

    DEFF Research Database (Denmark)

    Pyndt Jørgensen, Bettina; Winther, Gudrun; Kihl, Pernille

    2015-01-01

    and whether there was a link between the two. A total of 20 C57BL/6 mice, fed either a standard diet or a magnesium-deficient diet for 6 weeks, were tested using the light-dark box anxiety test. Gut microbiota composition was analysed by denaturation gradient gel electrophoresis. RESULTS: We demonstrated...

  10. Effects of Differing Response-Force Requirements on Food-Maintained Responding in C57BL/6J Mice

    Science.gov (United States)

    Zarcone, Troy J.; Chen, Rong; Fowler, Stephen C.

    2009-01-01

    The effect of force requirements on response effort was examined using inbred C57BL/6J mice trained to press a disk with their snout. Lateral peak forces greater than 2 g were defined as responses (i.e., all responses above the measurement threshold). Different, higher force requirements were used to define criterion responses (a subclass of all…

  11. Observations of low and intermediate-frequency-peaked BL Lacs above 100 GeV with VERITAS

    Directory of Open Access Journals (Sweden)

    Errando M.

    2013-12-01

    Full Text Available Most of the ~ 50 blazars detected to date at TeV energies (E > 0.1 TeV are high-frequency-peaked BL Lacs (HBLs. Only a handful episodic detections of low- and intermediate-frequency-peaked BL Lacs (LBL/IBLs, with synchrotron peak frequencies in the infrared and optical regime have been reported by ground-based gamma-ray telescopes, typically during high-flux states. The VERITAS array located in southern Arizona has observed five known TeV LBL/IBLs since 2009: 3C 66A, WComae, PKS 1424+240, S5 0716+714 and BL Lacertae, with exposures of 5-10 hours/year, which so far resulted in the detection of a bright, sub-hour timescale gamma-ray flare of BL Lacertae in June 2011. We also report the detection and characterization of two new IBLs: VER J0521+211 and B2 1215+30.

  12. Regional distribution of biogenic amines, amino acids and cholinergic markers in the CNS of the C57BL/6 strain

    NARCIS (Netherlands)

    Van Dam, D; Marescau, B; Cremers, T; Mulder, J; Engelborghs, S; De Deyn, PP

    A reliable extrapolation of neurochemical alterations from a mouse model to human metabolic brain disease requires knowledge of neurotransmitter levels and related compounds in control mouse brain. C57BL/6 is a widely used background strain for knockout and transgenic mouse models. A prerequisite

  13. Soft X-Ray Observations of a Complete Sample of X-Ray--selected BL Lacertae Objects

    Science.gov (United States)

    Perlman, Eric S.; Stocke, John T.; Wang, Q. Daniel; Morris, Simon L.

    1996-01-01

    We present the results of ROSAT PSPC observations of the X-ray selected BL Lacertae objects (XBLs) in the complete Einstein Extended Medium Sensitivity Survey (EM MS) sample. None of the objects is resolved in their respective PSPC images, but all are easily detected. All BL Lac objects in this sample are well-fitted by single power laws. Their X-ray spectra exhibit a variety of spectral slopes, with best-fit energy power-law spectral indices between α = 0.5-2.3. The PSPC spectra of this sample are slightly steeper than those typical of flat ratio-spectrum quasars. Because almost all of the individual PSPC spectral indices are equal to or slightly steeper than the overall optical to X-ray spectral indices for these same objects, we infer that BL Lac soft X-ray continua are dominated by steep-spectrum synchrotron radiation from a broad X-ray jet, rather than flat-spectrum inverse Compton radiation linked to the narrower radio/millimeter jet. The softness of the X-ray spectra of these XBLs revives the possibility proposed by Guilbert, Fabian, & McCray (1983) that BL Lac objects are lineless because the circumnuclear gas cannot be heated sufficiently to permit two stable gas phases, the cooler of which would comprise the broad emission-line clouds. Because unified schemes predict that hard self-Compton radiation is beamed only into a small solid angle in BL Lac objects, the steep-spectrum synchrotron tail controls the temperature of the circumnuclear gas at r ≤ 1018 cm and prevents broad-line cloud formation. We use these new ROSAT data to recalculate the X-ray luminosity function and cosmological evolution of the complete EMSS sample by determining accurate K-corrections for the sample and estimating the effects of variability and the possibility of incompleteness in the sample. Our analysis confirms that XBLs are evolving "negatively," opposite in sense to quasars, with Ve/Va = 0.331±0.060. The statistically significant difference between the values for X

  14. 76 FR 61351 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-10-04

    ... MBR Baseline Tariff Filing to be effective 9/22/2011. Filed Date: 09/22/2011. Accession Number... submits tariff filing per 35.1: ECNY MBR Re-File to be effective 9/22/2011. Filed Date: 09/22/2011... Industrial Energy Buyers, LLC submits tariff filing per 35.1: NYIEB MBR Re-File to be effective 9/22/2011...

  15. Idiopathic paraproteinemia. III. Increased frequency of paraproteinemia in thymectomized aging C57BL/KaLwRij and CBA/BrARij mice

    NARCIS (Netherlands)

    Radl, J.; Glopper, E. de; Berg, P. van den; Zwieten, M.J. van

    1980-01-01

    The influence of thymectomy on the appearance of idiopathic paraproteinemia (IP) during aging was investigated in mice of the C57BL/KaLwRij and CBA/BrARij strains, which under normal conditions develop IP in high and low frequency, respectively. Compared with sham-thymectomized mice, C57BL mice

  16. Indomethacin treatment prevents diet-induced obesity and insulin resistance, but not glucose intolerance in C57BL/6J mice

    Science.gov (United States)

    OBJECTIVE: We performed experiments to examine the metabolic consequences of inhibition of cyclooxygenase (COX) activity in obesity-prone C57BL/6J mice fed a high fat/high sucrose (HF/HS) diet. RESEARCH DESIGN AND METHODS: C57BL/6J mice were fed a HF/HS diet for 7 weeks under thermoneutral conditio...

  17. Construction of a Wide-range High-resolution Beamline BL05 in NewSUBARU for Soft X-ray Spectroscopic Analysis on Industrial Materials

    International Nuclear Information System (INIS)

    Kanda, K; Hasegawa, T; Uemura, M; Niibe, M; Haruyama, Y; Motoyama, M; Amemiya, K; Fukushima, S; Ohta, T

    2013-01-01

    A material analysis beamline which is designed to useable in the wide energy range, 50∼4000 eV for the various industrial demands, was constructed at BL05 in NewSUBARU for industrial companies' use in cooperation with the Laboratory of Advanced Science and Technology for Industry (LASTI), University of Hyogo and well-informed researchers in the industrial world. BL05 consists of two branch lines, which is mounted with a double crystal monochromator at BL05A and grating monochromator at BL05B. XAFS measurements in the total electron yield mode and partial fluorescence yield mode using a silicon drift detector can be performed at the both branch lines. In addition, the photoelectron spectra can be measured at BL05B. The maintenance and management of BL05 are performed by Synchrotron Analysis LLC (SALLC), which is a consortium of user companies of BL05, under the supervision of the LASTI staff. If industrial users want help with measurements at BL05, operators of SALLC can assist them.

  18. Construction of a Wide-range High-resolution Beamline BL05 in NewSUBARU for Soft X-ray Spectroscopic Analysis on Industrial Materials

    Science.gov (United States)

    Kanda, K.; Hasegawa, T.; Uemura, M.; Niibe, M.; Haruyama, Y.; Motoyama, M.; Amemiya, K.; Fukushima, S.; Ohta, T.

    2013-03-01

    A material analysis beamline which is designed to useable in the wide energy range, 50~4000 eV for the various industrial demands, was constructed at BL05 in NewSUBARU for industrial companies' use in cooperation with the Laboratory of Advanced Science and Technology for Industry (LASTI), University of Hyogo and well-informed researchers in the industrial world. BL05 consists of two branch lines, which is mounted with a double crystal monochromator at BL05A and grating monochromator at BL05B. XAFS measurements in the total electron yield mode and partial fluorescence yield mode using a silicon drift detector can be performed at the both branch lines. In addition, the photoelectron spectra can be measured at BL05B. The maintenance and management of BL05 are performed by Synchrotron Analysis LLC (SALLC), which is a consortium of user companies of BL05, under the supervision of the LASTI staff. If industrial users want help with measurements at BL05, operators of SALLC can assist them.

  19. Diverse manifestations of tumorigenicity and immunogenicity displayed by the poorly immunogenic B16-BL6 melanoma transduced with cytokine genes.

    Science.gov (United States)

    Arca, M J; Krauss, J C; Strome, S E; Cameron, M J; Chang, A E

    1996-05-01

    We evaluated the in vivo response to the poorly immunogenic B16-BL6 (BL6) murine melanoma genetically altered to secrete interleukin-2 (IL-2), IL-4, interferon gamma (IFN gamma) and granulocyte/macrophage-colony-stimulating factor (GM-CSF). Three parameters were evaluated: (1) tumorigenicity, (2) vaccination of naive animals, and (3) assessment of antitumor reactivity of T cells derived from tumor-draining lymph nodes (TDLN). Secretion of IL-2 abrogated the tumorigenicity of BL6, while IFN gamma and IL-4 partially reduced tumorigenicity, and GM-CSF had no effect. Protective immunity to wild-type tumor challenge could not be achieved by vaccination with irradiated cytokine-secreting tumors, although IL-2 and IL-4 secretion appeared to retard the growth of the challenge inoculum significantly. An alternative method to evaluate the immunogenicity of the cytokine-secreting tumors was to measure the ability of T cells obtained from TDLN to mediate regression of wild-type tumor in adoptive immunotherapy. Neither IL-2 nor IFN gamma secretion resulted in the induction of immune T cells. By contrast, GM-CSF and IL-4 secretion were found to induce immune T cells in the TDLN with GM-CSF being superior to IL-4. The combined secretion of GM-CSF and IL-4 did not lead to enhanced induction of immune T cells. GM-CSF secretion was found to upregulate B7-1 expression in TDLN, consistent with an increase in the population of antigen-presenting cells. These studies demonstrated that reduced tumorigenicity by cytokine secretion did not correlate with increased immunogenicity. With the cytokines examined, there was limited capability of developing protective immunity against the BL6 tumor. Nevertheless, GM-CSF and IL-4 secretion significantly enhanced T cell immune reactivity to the poorly immunogenic BL6 tumor.

  20. Iterative solution of multiple radiation and scattering problems in structural acoustics using the BL-QMR algorithm

    Energy Technology Data Exchange (ETDEWEB)

    Malhotra, M. [Stanford Univ., CA (United States)

    1996-12-31

    Finite-element discretizations of time-harmonic acoustic wave problems in exterior domains result in large sparse systems of linear equations with complex symmetric coefficient matrices. In many situations, these matrix problems need to be solved repeatedly for different right-hand sides, but with the same coefficient matrix. For instance, multiple right-hand sides arise in radiation problems due to multiple load cases, and also in scattering problems when multiple angles of incidence of an incoming plane wave need to be considered. In this talk, we discuss the iterative solution of multiple linear systems arising in radiation and scattering problems in structural acoustics by means of a complex symmetric variant of the BL-QMR method. First, we summarize the governing partial differential equations for time-harmonic structural acoustics, the finite-element discretization of these equations, and the resulting complex symmetric matrix problem. Next, we sketch the special version of BL-QMR method that exploits complex symmetry, and we describe the preconditioners we have used in conjunction with BL-QMR. Finally, we report some typical results of our extensive numerical tests to illustrate the typical convergence behavior of BL-QMR method for multiple radiation and scattering problems in structural acoustics, to identify appropriate preconditioners for these problems, and to demonstrate the importance of deflation in block Krylov-subspace methods. Our numerical results show that the multiple systems arising in structural acoustics can be solved very efficiently with the preconditioned BL-QMR method. In fact, for multiple systems with up to 40 and more different right-hand sides we get consistent and significant speed-ups over solving the systems individually.

  1. Computer Forensics Method in Analysis of Files Timestamps in Microsoft Windows Operating System and NTFS File System

    Directory of Open Access Journals (Sweden)

    Vesta Sergeevna Matveeva

    2013-02-01

    Full Text Available All existing file browsers displays 3 timestamps for every file in file system NTFS. Nowadays there are a lot of utilities that can manipulate temporal attributes to conceal the traces of file using. However every file in NTFS has 8 timestamps that are stored in file record and used in detecting the fact of attributes substitution. The authors suggest a method of revealing original timestamps after replacement and automated variant of it in case of a set of files.

  2. DICOM supported sofware configuration by XML files

    International Nuclear Information System (INIS)

    LucenaG, Bioing Fabian M; Valdez D, Andres E; Gomez, Maria E; Nasisi, Oscar H

    2007-01-01

    A method for the configuration of informatics systems that provide support to DICOM standards using XML files is proposed. The difference with other proposals is base on that this system does not code the information of a DICOM objects file, but codes the standard itself in an XML file. The development itself is the format for the XML files mentioned, in order that they can support what DICOM normalizes for multiple languages. In this way, the same configuration file (or files) can be use in different systems. Jointly the XML configuration file generated, we wrote also a set of CSS and XSL files. So the same file can be visualized in a standard browser, as a query system of DICOM standard, emerging use, that did not was a main objective but brings a great utility and versatility. We exposed also some uses examples of the configuration file mainly in relation with the load of DICOM information objects. Finally, at the conclusions we show the utility that the system has already provided when the edition of DICOM standard changes from 2006 to 2007

  3. Multi-epoch intranight optical monitoring of eight radio-quiet BL Lac candidates

    Science.gov (United States)

    Kumar, P.; Gopal-Krishna; Stalin, C. S.; Chand, H.; Srianand, R.; Petitjean, P.

    2017-10-01

    For a new sample of eight weak-line quasars (WLQs) we report a sensitive search in 20 intranight monitoring sessions, for blazar-like optical flux variations on hour-like and longer time-scale (day/month/year-like). The sample consists exclusively of the WLQs that are not radio-loud and either have been classified as 'radio-weak probable BL Lac candidates' and/or are known to have exhibited at least one episode of large, blazar-like optical variability. Whereas only a hint of intranight variability is seen for two of these WLQs, J104833.5+620305.0 (z = 0.219) and J133219.6+622715.9 (z = 3.15), statistically significant internight variability at a few per cent level is detected for three of the sources, including the radio-intermediate WLQ J133219.6+622715.9 (z = 3.15) and the well-known bona fide radio-quiet WLQs J121221.5+534128.0 (z = 3.10) and WLQ J153259.9-003944.1 (z = 4.62). In the rest frame, this variability is intraday and in the far-ultraviolet band. On the time-scale of a decade, we find for three of the WLQs large brightness changes, amounting to 1.655 ± 0.009, 0.163 ± 0.010 and 0.144 ± 0.018 mag, for J104833.5+620305.0, J123743.1+630144.9 and J232428.4+144324.4, respectively. Whereas the latter two are confirmed radio-quiet WLQs, the extragalactic nature of J104833.5+620305.0 remains to be well established, thanks to the absence of any feature(s) in its available optical spectra. This study forms a part of our ongoing campaign of intranight optical monitoring of radio-quiet WLQs, in order to improve the understanding of this enigmatic class of active galactic nuclei and to look among them for a possible tiny, elusive population of radio-quiet BL Lacs.

  4. Short timescale photometric and polarimetric behavior of two BL Lacertae type objects

    International Nuclear Information System (INIS)

    Covino, S.; Baglio, M. C.; Foschini, L.; Sandrinelli, A.; Tavecchio, F.; Treves, A.; Zhang, H.; Barres de Almeida, U.; Bonnoli, G.; Boettcher, M.; Cecconi, M.; D'Ammando, F.; Fabrizio, L. di; Giarrusso, M.; Leone, F.; Lindfors, E.; Lorenzi, V.; Molinari, E.; Paiano, S.; Prandini, E.; Raiteri, C. M.; Stamerra, A.; Tagliaferri, G.

    2015-01-01

    Context. Blazars are astrophysical sources whose emission is dominated by non-thermal processes, typically interpreted as synchrotron and inverse Compton emission. Although the general picture is rather robust and consistent with observations, many aspects are still unexplored. Aims. Polarimetric monitoring can offer a wealth of information about the physical processes in blazars. Models with largely different physical ingredients can often provide almost indistinguishable predictions for the total flux, but usually are characterized by markedly different polarization properties. We explore, with a pilot study, the possibility to derive structural information about the emitting regions of blazars by means of a joint analysis of rapid variability of the total and polarized flux at optical wavelengths. Methods. Short timescale (from tens of seconds to a couple of minutes) optical linear polarimetry and photometry for two blazars, BL Lacertae and PKS 1424+240, was carried out with the PAOLO polarimeter at the 3.6 m Telescopio Nazionale Galileo. Several hours of almost continuous observations were obtained for both sources. Results. Our intense monitoring allowed us to draw strongly different scenarios for BL Lacertae and PKS 1424+240, with the former characterized by intense variability on time-scales from hours to a few minutes and the latter practically constant in total flux. Essentially the same behavior is observed for the polarized flux and the position angle. The variability time-scales turned out to be as short as a few minutes, although involving only a few percent variation of the flux. The polarization variability time-scale is generally consistent with the total flux variability. Total and polarized flux appear to be essentially uncorrelated. However, even during our relatively short monitoring, different regimes can be singled out. Conclusions. No simple scenario is able to satisfactorily model the very rich phenomenology exhibited in our data. As a

  5. Efecto de la ciclosporina A en ratones C57BL/6 infectados con Encephalitozoon intestinalis.

    Directory of Open Access Journals (Sweden)

    Ana Luz Galván

    2006-03-01

    Full Text Available Introducción. Encephalitozoon intestinalis es un microsporidio parásito del intestino, que puede diseminarse en pacientes inmunocomprometidos. Existen referencias de modelos animales inmunosuprimidos para el estudio de la microsporidiosis utilizando fármacos que producen supresión total de la respuesta inmune; sin embargo, no se han estudiado los efectos de inmunosupresores con acción selectiva sobre los componentes de esta respuesta. Objetivo. Evaluar el efecto de la inmunosupresión con ciclosporina A (CsA en ratones C57BL/6 infectados con E. intestinalis. Materiales y métodos. Se utilizaron 80 ratones C57BL/6 distribuidos en cuatro grupos: infectados, inmunosuprimidos e infectados, inmunosuprimidos no infectados y controles. La inmunosupresión con CsA (50 mg/kg se realizó vía intraperitoneal durante todo el estudio. En la semanas 2, 3, 4 y 6 posteriores a la infección se obtuvo sangre para determinar los anticuerpos, y materia fecal para evaluar la cinética de excreción de esporas. Además, se extrajeron varios órganos para estudiar la histopatología y observar la posible diseminación del parásito. Resultados. La producción de anticuerpos IgG fue mayor en los ratones inmunocompetentes infectados que en los inmunosuprimidos infectados con E. intestinalis. No se encontró el parásito en órganos diferentes al intestino delgado en los dos grupos infectados. Sin embargo, la excreción de esporas, tanto en heces como en líquido duodenal, fue mayor en el grupo inmunosuprimido infectado. Conclusión. La CsA en el modelo en ratón no indujo la diseminación de E. intestinalis ni la exacerbación de la enfermedad, pero contribuyó al aumento en la cinética de excreción de esporas y la disminución de la producción de anticuerpos IgG en los ratones inmunosuprimidos infectados.

  6. Erionite induces production of autoantibodies and IL-17 in C57BL/6 mice

    International Nuclear Information System (INIS)

    Zebedeo, Christian Nash; Davis, Chad; Peña, Cecelia; Ng, Kok Wei; Pfau, Jean C.

    2014-01-01

    Background: Erionite has similar chemical and physical properties to amphibole asbestos, which induces autoantibodies in mice. Current exposures are occurring in North Dakota due to the use of erionite-contaminated gravel. While erionite is known to cause mesothelioma and other diseases associated with asbestos, there is little known about its effects on the immune system. Objectives: We performed this study to determine whether erionite evokes autoimmune reactions in mice. Methods: Bone marrow derived macrophages (BMDM) were used to measure toxicity induced by erionite. Cytokine production by BMDM and splenocytes of C57BL/6 mice was examined by bead arrays and ELISA following exposure to erionite, amphiboles and chrysotile. Wild type C57BL/6 mice were exposed to saline, erionite, amphibole asbestos (Libby 6-Mix) or chrysotile through intratracheal instillations at equal mass (60 μg/mouse). Seven months after exposure, sera were examined for anti-nuclear antibodies (ANA) and IL-17. Immunohistochemistry was used to detect immune complex deposition in the kidneys. Results: Erionite and tremolite caused increased cytokine production belonging to the T H 17 profile including IL-17, IL-6, TGF-β, and TNF-α. The frequency of ANA was increased in mice treated with erionite or amphibole compared to saline-treated mice. IL-17 and TNF-α were elevated in the sera of mice treated with erionite. The frequency of immune complex deposition in the kidneys increased from 33% in saline-treated mice to 90% with erionite. Conclusions: These data demonstrate that both erionite and amphibole asbestos induce autoimmune responses in mice, suggesting a potential for adverse effects in exposed communities. - Highlights: • Erionite, a fibrous mineral, is a current public health concern in the western USA. • Erionite exposure induces antinuclear autoantibodies in exposed mice. • Erionite induces a clear Th17 cytokine response in vitro and in vivo. • These responses were distinct

  7. Erionite induces production of autoantibodies and IL-17 in C57BL/6 mice

    Energy Technology Data Exchange (ETDEWEB)

    Zebedeo, Christian Nash; Davis, Chad [Department of Biological Sciences, Idaho State University, Pocatello, ID (United States); Peña, Cecelia [Northwest Nazarene University, Nampa, ID (United States); Ng, Kok Wei [Department of Biological Sciences, Idaho State University, Pocatello, ID (United States); Pfau, Jean C., E-mail: pfaujean@isu.edu [Department of Biological Sciences, Idaho State University, Pocatello, ID (United States)

    2014-03-15

    Background: Erionite has similar chemical and physical properties to amphibole asbestos, which induces autoantibodies in mice. Current exposures are occurring in North Dakota due to the use of erionite-contaminated gravel. While erionite is known to cause mesothelioma and other diseases associated with asbestos, there is little known about its effects on the immune system. Objectives: We performed this study to determine whether erionite evokes autoimmune reactions in mice. Methods: Bone marrow derived macrophages (BMDM) were used to measure toxicity induced by erionite. Cytokine production by BMDM and splenocytes of C57BL/6 mice was examined by bead arrays and ELISA following exposure to erionite, amphiboles and chrysotile. Wild type C57BL/6 mice were exposed to saline, erionite, amphibole asbestos (Libby 6-Mix) or chrysotile through intratracheal instillations at equal mass (60 μg/mouse). Seven months after exposure, sera were examined for anti-nuclear antibodies (ANA) and IL-17. Immunohistochemistry was used to detect immune complex deposition in the kidneys. Results: Erionite and tremolite caused increased cytokine production belonging to the T{sub H}17 profile including IL-17, IL-6, TGF-β, and TNF-α. The frequency of ANA was increased in mice treated with erionite or amphibole compared to saline-treated mice. IL-17 and TNF-α were elevated in the sera of mice treated with erionite. The frequency of immune complex deposition in the kidneys increased from 33% in saline-treated mice to 90% with erionite. Conclusions: These data demonstrate that both erionite and amphibole asbestos induce autoimmune responses in mice, suggesting a potential for adverse effects in exposed communities. - Highlights: • Erionite, a fibrous mineral, is a current public health concern in the western USA. • Erionite exposure induces antinuclear autoantibodies in exposed mice. • Erionite induces a clear Th17 cytokine response in vitro and in vivo. • These responses were

  8. Panorama 98. 16 thematic files

    International Nuclear Information System (INIS)

    Anon.

    1998-01-01

    Under the 'Panorama 98' title, the Institut Francais du Petrole (IFP) has organized an international colloquium on the most recent events and the foreseeable perspectives in the domain of hydrocarbons. 16 technical and economical documentation files were given to the participants. This paper presents a synthesis of the content of these files: the oil offer and demand, the worldwide petroleum activity, the recent oil and gas discoveries, the stakes of the oil and gas perspectives in the North Sea, the US renewal in the Gulf of Mexico, the exploitation of heavy and extra-heavy crudes in Venezuela, the Asian oil rise, the limits of oil recovery efficiency, the oil markets, the international overview of the natural gas industry, the perspectives of the LNG market, the recent evolution and the perspectives of the European refining activities, the oil companies in 1997, the future of marketing, the trends of European automotive industry, and the scientific certitudes and uncertainties about urban pollution. (J.S.)

  9. Computerized index for teaching files

    International Nuclear Information System (INIS)

    Bramble, J.M.

    1989-01-01

    A computerized index can be used to retrieve cases from a teaching file that have radiographic findings similar to an unknown case. The probability that a user will review cases with a correct diagnosis was estimated with use of radiographic findings of arthritis in hand radiographs of 110 cases from a teaching file. The nearest-neighbor classification algorithm was used as a computer index to 110 cases of arthritis. Each case was treated as an unknown and inputted to the computer index. The accuracy of the computer index in retrieving cases with the same diagnosis (including rheumatoid arthritis, gout, psoriatic arthritis, inflammatory osteoarthritis, and pyrophosphate arthropathy) was measured. A Bayes classifier algorithm was also tested on the same database. Results are presented. The nearest-neighbor algorithm was 83%. By comparison, the estimated accuracy of the Bayes classifier algorithm was 78%. Conclusions: A computerized index to a teaching file based on the nearest-neighbor algorithm should allow the user to review cases with the correct diagnosis of an unknown case, by entering the findings of the unknown case

  10. Small file aggregation in a parallel computing system

    Science.gov (United States)

    Faibish, Sorin; Bent, John M.; Tzelnic, Percy; Grider, Gary; Zhang, Jingwang

    2014-09-02

    Techniques are provided for small file aggregation in a parallel computing system. An exemplary method for storing a plurality of files generated by a plurality of processes in a parallel computing system comprises aggregating the plurality of files into a single aggregated file; and generating metadata for the single aggregated file. The metadata comprises an offset and a length of each of the plurality of files in the single aggregated file. The metadata can be used to unpack one or more of the files from the single aggregated file.

  11. Flat Files - JSNP | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ... Data file File name: jsnp_flat_files File URL: ftp://ftp.biosciencedbc.jp/archiv...his Database Database Description Download License Update History of This Database Site Policy | Contact Us Flat Files - JSNP | LSDB Archive ...

  12. 10 CFR 110.89 - Filing and service.

    Science.gov (United States)

    2010-01-01

    ...: Rulemakings and Adjudications Staff or via the E-Filing system, following the procedure set forth in 10 CFR 2.302. Filing by mail is complete upon deposit in the mail. Filing via the E-Filing system is completed... residence with some occupant of suitable age and discretion; (2) Following the requirements for E-Filing in...

  13. 49 CFR 1104.6 - Timely filing required.

    Science.gov (United States)

    2010-10-01

    ... offers next day delivery to Washington, DC. If the e-filing option is chosen (for those pleadings and documents that are appropriate for e-filing, as determined by reference to the information on the Board's Web site), then the e-filed pleading or document is timely filed if the e-filing process is completed...

  14. 12 CFR 908.25 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Filing of papers. 908.25 Section 908.25 Banks... RULES OF PRACTICE AND PROCEDURE IN HEARINGS ON THE RECORD General Rules § 908.25 Filing of papers. (a) Filing. Any papers required to be filed shall be addressed to the presiding officer and filed with the...

  15. 47 CFR 61.14 - Method of filing publications.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Method of filing publications. 61.14 Section 61...) TARIFFS Rules for Electronic Filing § 61.14 Method of filing publications. (a) Publications filed... date of a publication received by the Electronic Tariff Filing System will be determined by the date...

  16. 29 CFR 1981.103 - Filing of discrimination complaint.

    Science.gov (United States)

    2010-07-01

    ... constitute the violations. (c) Place of filing. The complaint should be filed with the OSHA Area Director... or she has been discriminated against by an employer in violation of the Act may file, or have filed..., but may be filed with any OSHA officer or employee. Addresses and telephone numbers for these...

  17. 77 FR 28592 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-05-15

    ...: Middletown MBR Application to be effective 5/8/2012. Filed Date: 5/7/12. Accession Number: 20120507-5128..., LLC. Description: Southern Energy Initial MBR Filing to be effective 5/ 7/2012. Filed Date: 5/8/12... Company submits tariff filing per 35.37: MBR Triennial Filing--1st Rev MBR to be effective 9/30/2010...

  18. 77 FR 66458 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-11-05

    ... Service Company of Colorado. Description: 2012--10--26 PSCo MBR Filing to be effective 12/26/ 2012. Filed...--SPS MBR Filing to be effective 12/26/2012. Filed Date: 10/26/12. Accession Number: 20121026-5123...: Revised Application for MBR Authorization to be effective 10/16/2012. Filed Date: 10/25/12. Accession...

  19. 75 FR 66075 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-10-27

    ....12: Baseline MBR Concurrence to be effective 10/8/2010. Filed Date: 10/19/2010. Accession Number... Company submits tariff filing per 35.12: Baseline MBR Concurrence to be effective 10/8/2010. Filed Date... Power Company submits tariff filing per 35.12: Baseline MBR Concurrence to be effective 10/8/2010. Filed...

  20. Degeneration of the osteocyte network in the C57BL/6 mouse model of aging.

    Science.gov (United States)

    Tiede-Lewis, LeAnn M; Xie, Yixia; Hulbert, Molly A; Campos, Richard; Dallas, Mark R; Dusevich, Vladimir; Bonewald, Lynda F; Dallas, Sarah L

    2017-10-26

    Age-related bone loss and associated fracture risk are major problems in musculoskeletal health. Osteocytes have emerged as key regulators of bone mass and as a therapeutic target for preventing bone loss. As aging is associated with changes in the osteocyte lacunocanalicular system, we focused on the responsible cellular mechanisms in osteocytes. Bone phenotypic analysis was performed in young-(5mo) and aged-(22mo) C57BL/6 mice and changes in bone structure/geometry correlated with alterations in osteocyte parameters determined using novel multiplexed-3D-confocal imaging techniques. Age-related bone changes analogous to those in humans were observed, including increased cortical diameter, decreased cortical thickness, reduced trabecular BV/TV and cortical porosities. This was associated with a dramatic reduction in osteocyte dendrite number and cell density, particularly in females, where osteocyte dendricity decreased linearly from 5, 12, 18 to 22mo and correlated significantly with cortical bone parameters. Reduced dendricity preceded decreased osteocyte number, suggesting dendrite loss may trigger loss of viability. Age-related degeneration of osteocyte networks may impair bone anabolic responses to loading and gender differences in osteocyte cell body and lacunar fluid volumes we observed in aged mice may lead to gender-related differences in mechanosensitivity. Therapies to preserve osteocyte dendricity and viability may be beneficial for bone health in aging.

  1. The effects of breeding protocol in C57BL/6J mice on adult offspring behaviour.

    Directory of Open Access Journals (Sweden)

    Claire J Foldi

    Full Text Available Animal experiments have demonstrated that a wide range of prenatal exposures can impact on the behaviour of the offspring. However, there is a lack of evidence as to whether the duration of sire exposure could affect such outcomes. We compared two widely used methods for breeding offspring for behavioural studies. The first involved housing male and female C57Bl/6J mice together for a period of time (usually 10-12 days and checking for pregnancy by the presence of a distended abdomen (Pair-housed; PH. The second involved daily introduction of female breeders to the male homecage followed by daily checks for pregnancy by the presence of vaginal plugs (Time-mated; TM. Male and female offspring were tested at 10 weeks of age on a behavioural test battery including the elevated plus-maze, hole board, light/dark emergence, forced swim test, novelty-suppressed feeding, active avoidance and extinction, tests for nociception and for prepulse inhibition (PPI of the acoustic startle response. We found that length of sire exposure (LSE had no significant effects on offspring behaviour, suggesting that the two breeding protocols do not differentially affect the behavioural outcomes of interest. The absence of LSE effects on the selected variables examined does not detract from the relevance of this study. Information regarding the potential influences of breeding protocol is not only absent from the literature, but also likely to be of particular interest to researchers studying the influence of prenatal manipulations on adult behaviour.

  2. Ajoene restored behavioral patterns and liver glutathione level in morphine treated C57BL6 mice.

    Science.gov (United States)

    Yun, Jaesuk; Oliynyk, Sergiy; Lee, Yeonju; Kim, Jieun; Yun, Kyunghwa; Jeon, Raok; Ryu, Jae-Ha; Oh, Seikwan

    2017-01-01

    Oxidative stress exacerbates drug dependence induced by administration of opiate analgesics such as morphine-induced tolerance and physical dependence associated with the reduction in hepatic glutathione (GSH) level. Ajoene obtained from garlic (Allium sativum L.) has been reported for anti-tumorigenic, anti-oxidative and neuroprotective properties, however, little is known about its effect on morphine-induced dependence. Therefore, this study aimed at the effect of ajoene on physical and/or psychological dependence and liver GSH content in morphine-treated mice. Conditioned place preference (CPP) test and measurement of morphine withdrawal syndrome were performed in C57BL6 mice for behavioral experiments. Thereafter, mice were sacrificed for measurement of serum and liver GSH levels. Ajoene restored CPP and naloxone-precipitated jumping behavior in mice exposed to morphine. Moreover, the reduced level of liver GSH content in morphine treated mice was back to normal after ajoene administration. Taken together, ajoene improved behavioral patterns in mice exposed to morphine suggesting its potential therapeutic benefit against morphine-induced dependence.

  3. Variable quasi-stellar sources with particular emphasis on objects of the BL Lac type

    International Nuclear Information System (INIS)

    Kinman, T.D.

    1975-01-01

    The optically variable quasars tend to have steep optical spectra and to show variable polarization; they tend to be associated with compact radio sources which have flat radio spectra at GHz frequencies. Objects are known which have continuous spectra (like BL Lac and OJ 287), but whose other properties closely parallel those of the variable quasars and N galaxies; in fact no sharp distinction can be drawn between them. The variation in the visibility of emission lines in quasars and N galaxies could be due to variations in the strength and spectral index of the radiation from the non-thermal source and from the differences in the amount and disposition of the material around it; it does not seem likely that a combination of these factors accounts for the observed range in emission line strength. The systematic difference in optical spectral index between continuous-spectrum objects (and OVV variables) on the one hand and those with emission lines on the other will produce a difference in K term between them, which may be expected to affect their distributions with respect to apparent magnitude. (Auth.)

  4. Neural stem cell isolation and culture from C57BL/6 mice

    Directory of Open Access Journals (Sweden)

    S Koirala

    2015-07-01

    Full Text Available INTRODUCTION A widely used in vitro culture, the neurosphere assay (NSA has provided a means to retrospectively identify neural progenitor cells as well as to determine both their selfrenewal capacity. Objective of study was to isolate and compare growth of the embryonic neuronal stem cell and adult neuronal stem cells in presence of Epidermal Growth Factor (EGF and Fibroblastic Growth Factor (FGF2. MATERIALS AND METHODS Embryonic neuronal stem cell were collected from cortical plate of dorsal telencephalon of fifteen C57BL/6 transgenic mice using stereoscopic microscope on 11th gestational day (GD. Adult mammalian neuronal stem cells taken from subventricular zone (SVZ of the lateral ventricles and subgranular layer of the dentate gyrus of the hippocampus were cultured. The growth for the neurosphere was then observed in interval of 24 and 72 hours. RESULT The adult stem cell culture showed few intact cells with high amount of debris and 9% heterogeneous sphere after 24 hours while only 20 % was observed at the end of 72 hours. Higher proliferation rate was observed in embryonic neurospheres than the adult stem cell culture. CONCLUSION Presence of EGF and basic FGF2 is essential for culture of neurospheres.DOI: http://dx.doi.org/10.3126/jcmsn.v10i2.12946 Journal of College of Medical Sciences-Nepal, 2014, Vol.10(2; 1-3

  5. Effects of Intermittent Fasting on Experimental Autoimune Encephalomyelitis in C57BL/6 Mice.

    Science.gov (United States)

    Razeghi Jahromi, Soodeh; Ghaemi, Amir; Alizadeh, Akram; Sabetghadam, Fatemeh; Moradi Tabriz, Hedieh; Togha, Mansoureh

    2016-06-01

    Several religions recommend periods of fasting. One of the most frequently asked questions of MS patients before the holy month of Ramadan is weather fasting might have an unfavorable effect on their disease course. This debate became more challenging after the publication of experimental studies suggesting that calorie restriction prior to disease induction attenuates disease severity. We conducted this study to assess early and late effects of fasting on the animal model of MS, known as autoimmune encephalomyelitis. EAE was induced in the C57BL/6 mice, using Myelin Oligodendrocyte Glycopeptide  (MOG) 35-55 and they fasted every other day either after the appearance of the first clinical sign or 30 days after disease induction for ten days. Thereafter, the mice were sacrificed for further histological and immunological evaluations. Intermittent fasting after the establishment of EAE did not have any unfavorable effect on the course of disease. Moreover, fasting at the early phase of disease alleviated EAE severity by ameliorating spinal cord demyelination. Fasting suppressed the secretion of IFN-γ, TNF-α and raised IL-10 production in splenocytes. Fasting was also associated with a lower percent of cytotoxicity. Intermittent fasting not only had no unfavorable effect on EAE but also reduced EAE severity if started at early phase of disease.

  6. Combination of vildagliptin and rosiglitazone ameliorates nonalcoholic fatty liver disease in C57BL/6 mice.

    Science.gov (United States)

    Mookkan, Jeyamurugan; De, Soumita; Shetty, Pranesha; Kulkarni, Nagaraj M; Devisingh, Vijayaraj; Jaji, Mallikarjun S; Lakshmi, Vinitha P; Chaudhary, Shilpee; Kulathingal, Jayanarayan; Rajesh, Navin B; Narayanan, Shridhar

    2014-01-01

    To evaluate the effect of vildagliptin alone and in combination with metformin or rosiglitazone on murine hepatic steatosis in diet-induced nonalcoholic fatty liver disease (NAFLD). Male C57BL/6 mice were fed with high fat diet (60 Kcal %) and fructose (40%) in drinking water for 60 days to induce NAFLD. After the induction period, animals were divided into different groups and treated with vildagliptin (10 mg/kg), metformin (350 mg/kg), rosiglitazone (10 mg/kg), vildagliptin (10 mg/kg) + metformin (350 mg/kg), or vildagliptin (10 mg/kg) + rosiglitazone (10 mg/kg) orally for 28 days. Following parameters were measured: body weight, food intake, plasma glucose, triglyceride (TG), total cholesterol, liver function tests, and liver TG. Liver histopathology was also examined. Oral administration of vildagliptin and rosiglitazone in combination showed a significant reduction in fasting plasma glucose, hepatic steatosis, and liver TGs. While other treatments showed less or no improvement in the measured parameters. These preliminary results demonstrate that administration of vildagliptin in combination with rosiglitazone could be a promising therapeutic strategy for the treatment of NAFLD.

  7. Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect

    Directory of Open Access Journals (Sweden)

    A. Paula Domínguez Rubio

    2017-09-01

    Full Text Available Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM and TEM. Similar to what has been described for other Gram-positive bacteria, MVs were on the nanometric size range (30–50 nm. MVs carried cytoplasmic components such as DNA, RNA and proteins. Using a proteomic approach (LC-MS, we identified a total of 103 proteins; 13 exclusively present in the MVs. The MVs content included cell envelope associated and secretory proteins, heat and cold shock proteins, several metabolic enzymes, proteases, structural components of the ribosome, membrane transporters, cell wall-associated hydrolases and phage related proteins. In particular, we identified proteins described as mediators of Lactobacillus’ probiotic effects such as p40, p75 and the product of LCABL_31160, annotated as an adhesion protein. The presence of these proteins suggests a role for the MVs in the bacteria-gastrointestinal cells interface. The expression and further encapsulation of proteins into MVs of GRAS (Generally Recognized as Safe bacteria could represent a scientific novelty, with applications in food, nutraceuticals and clinical therapies.

  8. Chronic pharmacologic inhibition of EGFR leads to cardiac dysfunction in C57BL/6J mice

    International Nuclear Information System (INIS)

    Barrick, Cordelia J.; Yu Ming; Chao, H.-H.; Threadgill, David W.

    2008-01-01

    Molecule-targeted therapies like those against the epidermal growth factor receptor (EGFR) are becoming widely used in the oncology clinic. With improvements in treatment efficacy, many cancers are being treated as chronic diseases, with patients having prolonged exposure to several therapies that were previously only given acutely. The consequence of chronic suppression of EGFR activity may lead to unexpected toxicities like altered cardiac physiology, a common organ site for adverse drug effects. To explore this possibility, we treated C57BL/6J (B6) mice with two EGFR small molecule tyrosine kinase inhibitors (TKIs), irreversible EKB-569 and reversible AG-1478, orally for 3 months. In B6 female mice, chronic exposure to both TKIs depressed body weight gain and caused significant changes in left ventricular (LV) wall thickness and cardiac function. No significant differences were observed in heart weight or cardiomyocyte size but histological analysis revealed an increase in fibrosis and in the numbers of TUNEL-positive cells in the hearts from treated female mice. Consistent with histological results, LV apoptotic gene expression was altered, with significant downregulation of the anti-apoptotic gene Bcl2l1. Although there were no significant differences in any of these endpoints in treated male mice, suggesting sex may influence susceptibility to TKI mediated toxicity, the LVs of treated male mice had significant upregulation of Egf, Erbb2 and Nppb over controls. Taken together, these data suggest that chronic dietary exposure to TKIs may result in pathological and physiological changes in the heart

  9. Development of a system for genetic manipulation of the facultative methanotroph Methylocella silvestris BL2.

    Science.gov (United States)

    Crombie, Andrew; Murrell, J Colin

    2011-01-01

    An understanding of the metabolism and metabolic regulation of the facultative methanotroph Methylocella silvestris BL2 is required to understand its role in methane oxidation in the environment, and methods for genetics manipulation are essential tools in these investigations. In addition, the ability to engineer the metabolic capabilities of M. silvestris may well have useful biotechnological applications. We describe a simple and effective method of genetic manipulation for this organism which relies on the electroporation of a linear DNA fragment to introduce chromosomal gene deletions. In a two-step procedure, the gene of interest is first replaced with an antibiotic-resistance cassette which is subsequently removed, resulting in an unmarked gene deletion. This method is illustrated by the deletion of isocitrate lyase, which abolished growth on one-carbon and severely disabled growth on two-carbon compounds. Subsequent complementation with the wild-type gene and promoter restored growth, demonstrating stable transcription from the broad-host-range plasmid employed. Copyright © 2011 Elsevier Inc. All rights reserved.

  10. BL4S, or how CERN sets the stage for teenage scientists

    CERN Multimedia

    Antonella Del Rosso

    2015-01-01

    Launched in 2014, the Beamline for Schools (BL4S) competition allows high-school students between 16 and 18 years old to run a real experiment at CERN’s PS accelerator (see here). For two years, students and schools worldwide have risen to the challenge and taken part enthusiastically in the competition. To ensure that it runs smoothly and enjoyably, over 100 CERN people work behind the scenes. The Bulletin lifts the curtain.   Student teams from Greece and the Netherlands – the winners of CERN’s first Beamline for schools competition – came to CERN to work on their experiments using a test beam. Turning young high-school students into real physicists who use a high-energy beam, set up an experiment and deal with data acquisition and analysis, is no game. For the people at CERN, the first step is to select the best proposals from those received from schools worldwide. “In 2015, over 40 scientists helped us select the best proposa...

  11. Improve beam position stability of SSRF BL15U beamline by using beam intensity feedback

    International Nuclear Information System (INIS)

    Li Guoqiang; Liang Dongxu; Yan Fen; Li Aiguo; Yu Xiaohan

    2013-01-01

    Background: The shaking of micro-focus spot in the vertical direction is found during the energy scan experiments, such as XAFS scan. The beam position of vertical direction changes obviously with the energy. Purpose: In order to make the beam position shaking amplitude less than 1/10 of the beam size. Methods: The beam position stability of SSRF BL15U beamline is improved by using beam intensity feedback. The feedback system include beam intensity monitor of the beamline and fine adjust mechanism of pitch 2 (the pitch angle of the second crystal of the double crystal monochromator). The feedback control of the beam position is realized by adjusting the pitch 2 to fix beam intensity at its maximum value. Results: The test results show that the vertical beam vibration below 10 Hz frequency is significantly reduced and also the beam position stability during photon energy scan is improved by more than 5 times. Conclusions: By adopting the new feedback systems, the stability of the beam spot on the specimen stage was dramatically improved which achieved the anticipated target. (authors)

  12. Aniracetam Does Not Alter Cognitive and Affective Behavior in Adult C57BL/6J Mice

    Science.gov (United States)

    Elston, Thomas W.; Pandian, Ashvini; Smith, Gregory D.; Holley, Andrew J.; Gao, Nanjing; Lugo, Joaquin N.

    2014-01-01

    There is a growing community of individuals who self-administer the nootropic aniracetam for its purported cognitive enhancing effects. Aniracetam is believed to be therapeutically useful for enhancing cognition, alleviating anxiety, and treating various neurodegenerative conditions. Physiologically, aniracetam enhances both glutamatergic neurotransmission and long-term potentiation. Previous studies of aniracetam have demonstrated the cognition-restoring effects of acute administration in different models of disease. No previous studies have explored the effects of aniracetam in healthy subjects. We investigated whether daily 50 mg/kg oral administration improves cognitive performance in naïve C57BL/6J mice in a variety of aspects of cognitive behavior. We measured spatial learning in the Morris water maze test; associative learning in the fear conditioning test; motor learning in the accelerating rotarod test; and odor discrimination. We also measured locomotion in the open field test, anxiety through the elevated plus maze test and by measuring time in the center of the open field test. We measured repetitive behavior through the marble burying test. We detected no significant differences between the naive, placebo, and experimental groups across all measures. Despite several studies demonstrating efficacy in impaired subjects, our findings suggest that aniracetam does not alter behavior in normal healthy mice. This study is timely in light of the growing community of healthy humans self-administering nootropic drugs. PMID:25099639

  13. Responses of Male C57BL/6N Mice to Observing the Euthanasia of Other Mice

    Science.gov (United States)

    Boivin, Gregory P; Bottomley, Michael A; Grobe, Nadja

    2016-01-01

    The AVMA Panel on Euthanasia recommends that sensitive animals should not be present during the euthanasia of others, especially of their own species, but does not provide guidelines on how to identify a sensitive species. To determine if mice are a sensitive species we reviewed literature on empathy in mice, and measured the cardiovascular and activity response of mice observing euthanasia of conspecifics. We studied male 16-wk-old C57BL/6N mice and found no increase in cardiovascular parameters or activity in the response of the mice to observing CO2 euthanasia. Mice observing decapitation had an increase in all values, but this was paralleled by a similar increase during mock decapitations in which no animals were handled or euthanized. We conclude that CO2 euthanasia of mice does not have an impact on other mice in the room, and that euthanasia by decapitation likely only has an effect due to the noise of the guillotine. We support the conceptual idea that mice are both a sensitive species and display empathy, but under the controlled circumstances of the euthanasia procedures used in this study there was no signaling of stress to witnessing inhabitants in the room. PMID:27423146

  14. Activation of basolateral amygdala in juvenile C57BL/6J mice during social approach behavior.

    Science.gov (United States)

    Ferri, Sarah L; Kreibich, Arati S; Torre, Matthew; Piccoli, Cara T; Dow, Holly; Pallathra, Ashley A; Li, Hongzhe; Bilker, Warren B; Gur, Ruben C; Abel, Ted; Brodkin, Edward S

    2016-10-29

    There is a strong need to better understand the neurobiology of juvenile sociability (tendency to seek social interaction), a phenotype of central relevance to autism spectrum disorders (ASD). Although numerous genetic mouse models of ASD showing reduced sociability have been reported, and certain brain regions, such as the amygdala, have been implicated in sociability, there has been little emphasis on delineating brain structures and circuits activated during social interactions in the critical juvenile period of the mouse strain that serves as the most common genetic background for these models-the highly sociable C57BL/6J (B6) strain. We measured expression of the immediate early genes Fos and Egr-1 to map activation of brain regions following the Social Approach Test (SAT) in juvenile male B6 mice. We hypothesized that juvenile B6 mice would show activation of the amygdala during social interactions. The basolateral amygdala (BLA) was activated by social exposure in highly sociable, 4-week-old B6 mice. In light of these data, and the many lines of evidence indicating alteration of amygdala circuits in human ASD, future studies are warranted to assess structural and functional alterations in the BLA, particularly at BLA synapses, in various mouse models of ASD. Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved.

  15. Effect of Modified Alkaline Supplementation on Syngenic Melanoma Growth in CB57/BL Mice.

    Science.gov (United States)

    Azzarito, Tommaso; Lugini, Luana; Spugnini, Enrico Pierluigi; Canese, Rossella; Gugliotta, Alessio; Fidanza, Stefano; Fais, Stefano

    2016-01-01

    Tumor extracellular acidity is a hallmark of malignant cancers. Thus, in this study we evaluated the effects of the oral administration of a commercially available water alkalizer (Basenpulver®) (BP) on tumor growth in a syngenic melanoma mouse model. The alkalizer was administered daily by oral gavage starting one week after tumor implantation in CB57/BL mice. Tumors were calipered and their acidity measured by in vivo MRI guided 31P MRS. Furthermore, urine pH was monitored for potential metabolic alkalosis. BP administration significantly reduced melanoma growth in mice; the optimal dose in terms of tolerability and efficacy was 8 g/l (p< 0.05). The in vivo results were supported by in vitro experiments, wherein BP-treated human and murine melanoma cell cultures exhibited a dose-dependent inhibition of tumor cell growth. This investigation provides the first proof of concept that systemic buffering can improve tumor control by itself and that this approach may represent a new strategy in prevention and/or treatment of cancers.

  16. Lactobacillus plantarum BL011 cultivation in industrial isolated soybean protein acid residue

    Directory of Open Access Journals (Sweden)

    Chaline Caren Coghetto

    Full Text Available Abstract In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87 g L-1, whereas lactic acid, the most important lactic acid bacteria metabolite, peaked at 37.59 g L-1, corresponding to a productivity of 1.46 g L-1 h-1. This is the first report on the use of liquid acid protein residue of soybean medium for L. plantarum growth. These results support the industrial use of this system as an alternative to produce probiotics without animal-derived ingredients to obtain high biomass concentrations in batch bioreactors.

  17. Lactobacillus plantarum BL011 cultivation in industrial isolated soybean protein acid residue.

    Science.gov (United States)

    Coghetto, Chaline Caren; Vasconcelos, Carolina Bettker; Brinques, Graziela Brusch; Ayub, Marco Antônio Záchia

    In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87gL -1 , whereas lactic acid, the most important lactic acid bacteria metabolite, peaked at 37.59gL -1 , corresponding to a productivity of 1.46gL -1 h -1 . This is the first report on the use of liquid acid protein residue of soybean medium for L. plantarum growth. These results support the industrial use of this system as an alternative to produce probiotics without animal-derived ingredients to obtain high biomass concentrations in batch bioreactors. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  18. Hair Growth-Promoting Effects of Lavender Oil in C57BL/6 Mice.

    Science.gov (United States)

    Lee, Boo Hyeong; Lee, Jae Soon; Kim, Young Chul

    2016-04-01

    The purpose of this study was to determine the hair growth effects of lavender oil (LO) in female C57BL/6 mice. The experimental animals were divided into a normal group (N: saline), a vehicle control group (VC: jojoba oil), a positive control group (PC: 3% minoxidil), experimental group 1 (E1: 3% LO), and experimental group 2 (E2: 5% LO). Test compound solutions were topically applied to the backs of the mice (100 μL per application), once per day, 5 times a week, for 4 weeks. The changes in hair follicle number, dermal thickness, and hair follicle depth were observed in skin tissues stained with hematoxylin and eosin, and the number of mast cells was measured in the dermal and hypodermal layers stained with toluidine blue. PC, E1, and E2 groups showed a significantly increased number of hair follicles, deepened hair follicle depth, and thickened dermal layer, along with a significantly decreased number of mast cells compared to the N group. These results indicated that LO has a marked hair growth-promoting effect, as observed morphologically and histologically. There was no significant difference in the weight of the thymus among the groups. However, both absolute and relative weights of the spleen were significantly higher in the PC group than in the N, VC, E1, or E2 group at week 4. Thus, LO could be practically applied as a hair growth-promoting agent.

  19. Effects of spaceflight on the immunoglobulin repertoire of unimmunized C57BL/6 mice

    Science.gov (United States)

    Ward, Claire; Rettig, Trisha A.; Hlavacek, Savannah; Bye, Bailey A.; Pecaut, Michael J.; Chapes, Stephen K.

    2018-02-01

    Spaceflight has been shown to suppress the adaptive immune response, altering the distribution and function of lymphocyte populations. B lymphocytes express highly specific and highly diversified receptors, known as immunoglobulins (Ig), that directly bind and neutralize pathogens. Ig diversity is achieved through the enzymatic splicing of gene segments within the genomic DNA of each B cell in a host. The collection of Ig specificities within a host, or Ig repertoire, has been increasingly characterized in both basic research and clinical settings using high-throughput sequencing technology (HTS). We utilized HTS to test the hypothesis that spaceflight affects the B-cell repertoire. To test this hypothesis, we characterized the impact of spaceflight on the unimmunized Ig repertoire of C57BL/6 mice that were flown aboard the International Space Station (ISS) during the Rodent Research One validation flight in comparison to ground controls. Individual gene segment usage was similar between ground control and flight animals, however, gene segment combinations and the junctions in which gene segments combine was varied among animals within and between treatment groups. We also found that spontaneous somatic mutations in the IgH and Igκ gene loci were not increased. These data suggest that space flight did not affect the B cell repertoire of mice flown and housed on the ISS over a short period of time.

  20. Aniracetam does not alter cognitive and affective behavior in adult C57BL/6J mice.

    Directory of Open Access Journals (Sweden)

    Thomas W Elston

    Full Text Available There is a growing community of individuals who self-administer the nootropic aniracetam for its purported cognitive enhancing effects. Aniracetam is believed to be therapeutically useful for enhancing cognition, alleviating anxiety, and treating various neurodegenerative conditions. Physiologically, aniracetam enhances both glutamatergic neurotransmission and long-term potentiation. Previous studies of aniracetam have demonstrated the cognition-restoring effects of acute administration in different models of disease. No previous studies have explored the effects of aniracetam in healthy subjects. We investigated whether daily 50 mg/kg oral administration improves cognitive performance in naïve C57BL/6J mice in a variety of aspects of cognitive behavior. We measured spatial learning in the Morris water maze test; associative learning in the fear conditioning test; motor learning in the accelerating rotarod test; and odor discrimination. We also measured locomotion in the open field test, anxiety through the elevated plus maze test and by measuring time in the center of the open field test. We measured repetitive behavior through the marble burying test. We detected no significant differences between the naive, placebo, and experimental groups across all measures. Despite several studies demonstrating efficacy in impaired subjects, our findings suggest that aniracetam does not alter behavior in normal healthy mice. This study is timely in light of the growing community of healthy humans self-administering nootropic drugs.

  1. SAP Suppresses the Development of Experimental Autoimmune Encephalomyelitis in C57BL6 Mice

    Science.gov (United States)

    Ji, Zhe; Ke, Zun-Ji; Geng, Jian-Guo

    2012-01-01

    Experimental autoimmune encephalomyelitis (EAE) is a CD4+ T cell-mediated disease of the CNS. Serum amyloid P component (SAP) is a highly conserved plasma protein named for its universal presence in amyloid deposits. Here we report SAP transgenic mice had unexpectedly attenuated EAE due to impaired encephalitogenic responses. Following induction with myelin oligodendroglial glycoprotein (MOG) peptide 35–55 in CFA, SAP transgenic mice showed reduced spinal cord inflammation with lower severity of EAE attacks as compared with control C57BL/6 mice. However in SAP-KO mice, the severity of EAE is enhanced. Adoptive transfer of Ag-restimulated T cells from wild-type to SAP transgenic mice or transfer of SAP transgenic Ag-restimulated T cells to control mice induced milder EAE. T cells from MOG-primed SAP transgenic mice showed weak proliferative responses. Furthermore, in SAP transgenic mice, there is little infiltration of CD45-positive cells in the spinal cord. In vitro, SAP suppressed the secretion of IL-2 stimulated by P-selectin, and blocked P-selectin binding to T cells. Moreover, SAP could change the affinity between α4-integrin and T cells. These data suggested that SAP could antagonize the development of the acute phase of inflammation accompanying EAE by modulating the function of P-selectin. PMID:21647172

  2. Embriogenesis somatik anggrek bulan Phalaenopsis amabilis (L. Bl: struktur dan pola perkembangan

    Directory of Open Access Journals (Sweden)

    Edy Setiti Wida Utami

    2012-02-01

    Full Text Available Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM e.i.: NP(New Phalaenopsis medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium e.i.: NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM. The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.

  3. Behavioral and neural correlates of acute and scheduled hunger in C57BL/6 mice.

    Directory of Open Access Journals (Sweden)

    Christian M Gallardo

    Full Text Available In rodents, daily feeding schedules induce food anticipatory activity (FAA rhythms with formal properties suggesting mediation by food-entrained circadian oscillators (FEOs. The search for the neuronal substrate of FEOs responsible for FAA is an active area of research, but studies spanning several decades have yet to identify unequivocally a brain region required for FAA. Variability of results across studies leads to questions about underlying biology versus methodology. Here we describe in C57BL/6 male mice the effects of varying the 'dose' of caloric restriction (0%, 60%, 80%, 110% on the expression of FAA as measured by a video-based analysis system, and on the induction of c-Fos in brain regions that have been implicated in FAA. We determined that more severe caloric restriction (60% leads to a faster onset of FAA with increased magnitude. Using the 60% caloric restriction, we found little evidence for unique signatures of neuronal activation in the brains of mice anticipating a daily mealtime compared to mice that were fasted acutely or fed ad-libitum-even in regions such as the dorsomedial and ventrolateral hypothalamus, nucleus accumbens, and cerebellum that have previously been implicated in FAA. These results underscore the importance of feeding schedule parameters in determining quantitative features of FAA in mice, and demonstrate dissociations between behavioral FAA and neural activity in brain areas thought to harbor FEOs or participate in their entrainment or output.

  4. Construction and performance of BL28 of the Photon Factory for circularly polarized synchrotron radiation

    International Nuclear Information System (INIS)

    Kagoshima, Y.; Muto, S.; Miyahara, T.; Koide, T.; Yamamoto, S.; Kitamura, H.

    1992-01-01

    A branch beamline, BL28A, has been constructed for the application of circularly polarized vacuum ultraviolet radiation. The radiation can be obtained in the helical undulator operation mode of an insertion device, EMPW number-sign 28, which is also cut for elliptically polarized hard x-ray radiation. T first harmonic of the helical undulator radiation can be tuned from 40 to 350 eV with its corresponding K value from 3 to 0.2. A monochromator working basically with constant deviation optics was installed, and has started its operation. A circularly polarized flux of ∼10 10 photons/s has been achieved with energy resolution of around 500--1000 at the first harmonic peak. The circular polarization after the monochromator was estimated to be higher than 70% by comparing theory and experiment on the magnetic circular dichroism of nickel films in the 3p-3d excitation region. The design philosophy of the beamline and recent results on the performance tests are presented

  5. [C57BL/6 mice open field behaviour qualitatively depends on arena size].

    Science.gov (United States)

    Lebedev, I V; Pleskacheva, M G; Anokhin, K V

    2012-01-01

    Open field behavior is well known to depend on physical characteristics of the apparatus. However many of such effects are poorly described especially with using of modern methods of behavioral registration and analysis. The previous results of experiments on the effect of arena size on behavior are not numerous and contradictory. We compared the behavioral scores of four groups of C57BL/6 mice in round open field arenas of four different sizes (diameter 35, 75, 150 and 220 cm). The behavior was registered and analyzed using Noldus EthoVision, WinTrack and SegmentAnalyzer software. A significant effect of arena size was found. Traveled distance and velocity increased, but not in proportion to increase of arena size. Moreover a significant effect on segment characteristics of the trajectory was revealed. Detailed behavior analysis revealed drastic differences in trajectory structure and number of rears between smaller (35 and 75 cm) and bigger (150 and 220 cm) arenas. We conclude, that the character of exploration in smaller and bigger arenas depends on relative size of central open zone in arena. Apparently its extension increases the motivational heterogeneity of space, that requires another than in smaller arenas, strategy of exploration.

  6. Serum antibody responses by male and female C57Bl/6 mice infected with Giardia muris.

    Science.gov (United States)

    Daniels, C W; Belosevic, M

    1994-09-01

    We compared the levels of serum antibodies in male and female C57Bl/6 mice during the primary and after challenge infection with Giardia muris. Male mice began passing cysts in their faeces earlier than females, and were shedding cysts for over 60 days, while females stopped shedding cysts by day 20 after infection. In both males and females there were significant increases in parasite-specific IgM 10 and 20 days after infection. No differences in parasite-specific serum IgA were observed until 40 days after infection. Parasite-specific IgG (whole) levels were elevated on days 20 and 40 in females, while males showed no significant increases. In addition, females had a much stronger IgG2b and IgG3 response than males. After challenge with either cysts or soluble parasite protein only the females had significant increases in specific anti-parasite IgG2b. Our data show differential ability of males and females to control the infection with G. muris is paralleled by a difference in the anti-parasite serum IgG response of the mice.

  7. Socially dominant mice in C57BL6 background show increased social motivation.

    Science.gov (United States)

    Kunkel, Thaddeus; Wang, Hongbing

    2018-01-15

    A series of behavioral tests measuring social dominance, social motivation, and non-social motivation are examined in adult male C57BL6 mice. By using the well-known tube dominance test to determine social dominance and rank, we find that, in the absence of competition for resource and mating, group-housed mouse cage-mates display stable and mostly linear and transitive social hierarchies. Mice with top and bottom social ranks are subjected to a three-chamber social interaction test to measure social motivation. The top ranked mice spend more time interacting with a stranger mouse than the bottom ranked mice, suggesting that social dominance may positively influence social motivation. When subjected to a novel environment, mice with different social ranks show similar locomotion and exploring activity in the open field test, suggesting no detectable difference in certain aspects of non-social motivation. These results demonstrate a behavioral correlation between social dominance and social motivation. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. ANTI-CORRELATED OPTICAL FLUX AND POLARIZATION VARIABILITY IN BL LAC

    Energy Technology Data Exchange (ETDEWEB)

    Gaur, Haritma [Inter-University Centre for Astronomy and Astrophysics (IUCAA), Ganeshkhind, Pune 411 007 (India); Gupta, Alok C. [Aryabhatta Research Institute of Observational Sciences (ARIES), Manora Peak, Nainital 263 129 (India); Wiita, Paul J. [Department of Physics, The College of New Jersey, P.O. Box 7718, Ewing, NJ 08628-0718 (United States); Uemura, Makoto; Itoh, Ryosuke; Sasada, Mahito, E-mail: haritma@iucaa.ernet.in [Hiroshima Astrophysical Science Center, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526 (Japan)

    2014-01-20

    We present the results of photometric (V band) and polarimetric observations of the blazar BL Lac during 2008-2010 using TRISPEC attached to the KANATA 1.5 m telescope in Japan. The data reveal a great deal of variability ranging from days to months with detection of strong variations in fractional polarization. The V band flux strongly anticorrelates with the degree of polarization during the first of two observing seasons but not during the second. The direction of the electric vector, however, remained roughly constant during all of our observations. These results are consistent with a model with at least two emission regions being present, with the more variable component having a polarization direction nearly perpendicular to that of the relatively quiescent region so that a rising flux can produce a decline in degree of polarization. We also computed models involving helical jet structures and single transverse shocks in jets and show that they might also be able to agree with the anticorrelations between flux and fractional polarization.

  9. [Combined effect of cisplatin and caffeine on murine B16-BL6 melanoma cells].

    Science.gov (United States)

    Yasutake, H; Tsuchiya, H; Sugihara, M; Tomita, K; Ueda, Y; Tanaka, M; Sasaki, T

    1989-05-01

    Combined effect of cisplatin and caffeine on murine B16-BL6 melanoma cells was studied. Synergistic inhibition of the cell growth was observed when caffeine (2 mM) was added continuously after one hour exposure of cisplatin. On the other hand, when caffeine was added before one hour exposure of cisplatin or one hour simultaneous exposure with cisplatin, synergistic effect was not shown. In the analysis of DNA histogram obtained from flow cytometry, S and G2/M accumulation was observed by the treatment of cisplatin and that accumulation was reduced by the combination of cisplatin and caffeine. From this findings, it was suggested that caffeine would inhibit DNA repair process. Furthermore, according to morphological studies with hematoxylin-eosin stain and Fontana-Masson stain, the addition of caffeine alone resulted in mild swelling of melanoma cells and the decrease of nuclear-cytoplasmic ratio. The combination of cisplatin and caffeine caused marked swelling of melanoma cells and remarkable increase of dendrite-like processes. Melanogenesis was also enhanced by the addition of these two drugs. Many matured melanosomes, increases of mitochondria, Golgi's apparatus and endoplasmic reticula were observed by the use of electron microscope. These findings implied that the combination of cisplatin and caffeine induced a differentiation of murine melanoma cells.

  10. Construction and performance of BL28 of the Photon Factory for circularly polarized synchrotron radiation

    Science.gov (United States)

    Kagoshima, Yasushi; Muto, Sadatsugu; Miyahara, Tsuneaki; Koide, Tsuneharu; Yamamoto, Shigeru; Kitamura, Hideo

    1992-01-01

    A branch beamline, BL28A, has been constructed for the application of circularly polarized vacuum ultraviolet radiation. The radiation can be obtained in the helical undulator operation mode of an insertion device, EMPW♯28, which is also cut for elliptically polarized hard x-ray radiation. T first harmonic of the helical undulator radiation can be tuned from 40 to 350 eV with its corresponding K value from 3 to 0.2. A monochromator working basically with constant deviation optics was installed, and has started its operation. A circularly polarized flux of ˜1010 photons/s has been achieved with energy resolution of around 500-1000 at the first harmonic peak. The circular polarization after the monochromator was estimated to be higher than 70% by comparing theory and experiment on the magnetic circular dichroism of nickel films in the 3p-3d excitation region. The design philosophy of the beamline and recent results on the performance tests are presented.

  11. Bone sarcoma induction by radium 224 in C57BL/Do mice

    International Nuclear Information System (INIS)

    Mays, C.W.; Lloyd, R.D.; Taylor, G.N.; Jones, C.W.

    1989-01-01

    Ninety-five C57BL/Do mice received radium 224 in ten weekly intraperitoneal injections. Doses ranged from 0.5 to 11.6 Gy. Twelve mice developed bone sarcoma. The risk coefficient ±SD was 2.8 ± 0.8%/Gy and the toxicity of shortlived radium 224 relative to longlived radium 226 was 5.4 ± 2.0. Concurrently, a single injection of 33 kBq/kg plutonium 239 was given to 47 similar mice which had a bone sarcoma risk coefficient of 8.4 ± 0.8%/Gy and toxicity relative to longlived radium 226 of 16 ± 4. Based on the studies of Mueller et al that established the increase in effectiveness with increased protraction of radium 224 dose, it is possible, if the radium 224 total dose had been spread continually over about 1 year, that the toxicity of the radium 224 might have been similar to that of plutonium 239. (author)

  12. Hepatitis B virus core antigen determines viral persistence in a C57BL/6 mouse model.

    Science.gov (United States)

    Lin, Yi-Jiun; Huang, Li-Rung; Yang, Hung-Chih; Tzeng, Horng-Tay; Hsu, Ping-Ning; Wu, Hui-Lin; Chen, Pei-Jer; Chen, Ding-Shinn

    2010-05-18

    We recently developed a mouse model of hepatitis B virus (HBV) persistence, in which a single i.v. hydrodynamic injection of HBV DNA to C57BL/6 mice allows HBV replication and induces a partial immune response, so that about 20-30% of the mice carry HBV for more than 6 months. The model was used to identify the viral antigen crucial for HBV persistence. We knocked out individual HBV genes by introducing a premature termination codon to the HBV core, HBeAg, HBx, and polymerase ORFs. The specific-gene-deficient HBV mutants were hydrodynamically injected into mice and the HBV profiles of the mice were monitored. About 90% of the mice that received the HBcAg-mutated HBV plasmid exhibited high levels of hepatitis B surface antigenemia and maintained HBsAg expression for more than 6 months after injection. To map the region of HBcAg essential for viral clearance, we constructed a set of serial HBcAg deletion mutants for hydrodynamic injection. We localized the essential region of HBcAg to the carboxyl terminus, specifically to the 10 terminal amino acids (HBcAg176-185). The majority of mice receiving this HBV mutant DNA did not elicit a proper HBcAg-specific IFN-gamma response and expressed HBV virions for 6 months. These results indicate that the immune response triggered in mice by HBcAg during exposure to HBV is important in determining HBV persistence.

  13. Effect of Modified Alkaline Supplementation on Syngenic Melanoma Growth in CB57/BL Mice.

    Directory of Open Access Journals (Sweden)

    Tommaso Azzarito

    Full Text Available Tumor extracellular acidity is a hallmark of malignant cancers. Thus, in this study we evaluated the effects of the oral administration of a commercially available water alkalizer (Basenpulver® (BP on tumor growth in a syngenic melanoma mouse model. The alkalizer was administered daily by oral gavage starting one week after tumor implantation in CB57/BL mice. Tumors were calipered and their acidity measured by in vivo MRI guided 31P MRS. Furthermore, urine pH was monitored for potential metabolic alkalosis. BP administration significantly reduced melanoma growth in mice; the optimal dose in terms of tolerability and efficacy was 8 g/l (p< 0.05. The in vivo results were supported by in vitro experiments, wherein BP-treated human and murine melanoma cell cultures exhibited a dose-dependent inhibition of tumor cell growth. This investigation provides the first proof of concept that systemic buffering can improve tumor control by itself and that this approach may represent a new strategy in prevention and/or treatment of cancers.

  14. Evaluated nuclear data file of Th-232

    International Nuclear Information System (INIS)

    Meadows, J.; Poenitz, W.; Smith, A.; Smith, D.; Whalen, J.; Howerton, R.

    1977-09-01

    An evaluated nuclear data file for thorium is described. The file extends over the energy range 0.049 (i.e., the inelastic-scattering threshold) to 20.0 MeV and is formulated within the framework of the ENDF system. The input data base, the evaluation procedures and judgments, and ancillary experiments carried out in conjunction with the evaluation are outlined. The file includes: neutron total cross sections, neutron scattering processes, neutron radiative capture cross sections, fission cross sections, (n;2n) and (n;3n) processes, fission properties (e.g., nu-bar and delayed neutron emission) and photon production processes. Regions of uncertainty are pointed out particularly where new measured results would be of value. The file is extended to thermal energies using previously reported resonance evaluations thereby providing a complete file for neutronic calculations. Integral data tests indicated that the file was suitable for neutronic calculations in the MeV range

  15. Flexibility and Performance of Parallel File Systems

    Science.gov (United States)

    Kotz, David; Nieuwejaar, Nils

    1996-01-01

    As we gain experience with parallel file systems, it becomes increasingly clear that a single solution does not suit all applications. For example, it appears to be impossible to find a single appropriate interface, caching policy, file structure, or disk-management strategy. Furthermore, the proliferation of file-system interfaces and abstractions make applications difficult to port. We propose that the traditional functionality of parallel file systems be separated into two components: a fixed core that is standard on all platforms, encapsulating only primitive abstractions and interfaces, and a set of high-level libraries to provide a variety of abstractions and application-programmer interfaces (API's). We present our current and next-generation file systems as examples of this structure. Their features, such as a three-dimensional file structure, strided read and write interfaces, and I/O-node programs, are specifically designed with the flexibility and performance necessary to support a wide range of applications.

  16. Storage of sparse files using parallel log-structured file system

    Science.gov (United States)

    Bent, John M.; Faibish, Sorin; Grider, Gary; Torres, Aaron

    2017-11-07

    A sparse file is stored without holes by storing a data portion of the sparse file using a parallel log-structured file system; and generating an index entry for the data portion, the index entry comprising a logical offset, physical offset and length of the data portion. The holes can be restored to the sparse file upon a reading of the sparse file. The data portion can be stored at a logical end of the sparse file. Additional storage efficiency can optionally be achieved by (i) detecting a write pattern for a plurality of the data portions and generating a single patterned index entry for the plurality of the patterned data portions; and/or (ii) storing the patterned index entries for a plurality of the sparse files in a single directory, wherein each entry in the single directory comprises an identifier of a corresponding sparse file.

  17. File Type Identification of File Fragments using Longest Common Subsequence (LCS)

    Science.gov (United States)

    Rahmat, R. F.; Nicholas, F.; Purnamawati, S.; Sitompul, O. S.

    2017-01-01

    Computer forensic analyst is a person in charge of investigation and evidence tracking. In certain cases, the file needed to be presented as digital evidence was deleted. It is difficult to reconstruct the file, because it often lost its header and cannot be identified while being restored. Therefore, a method is required for identifying the file type of file fragments. In this research, we propose Longest Common Subsequences that consists of three steps, namely training, testing and validation, to identify the file type from file fragments. From all testing results we can conlude that our proposed method works well and achieves 92.91% of accuracy to identify the file type of file fragment for three data types.

  18. A novel halophilic lipase, LipBL, showing high efficiency in the production of eicosapentaenoic acid (EPA.

    Directory of Open Access Journals (Sweden)

    Dolores Pérez

    Full Text Available BACKGROUND: Among extremophiles, halophiles are defined as microorganisms adapted to live and thrive in diverse extreme saline environments. These extremophilic microorganisms constitute the source of a number of hydrolases with great biotechnological applications. The interest to use extremozymes from halophiles in industrial applications is their resistance to organic solvents and extreme temperatures. Marinobacter lipolyticus SM19 is a moderately halophilic bacterium, isolated previously from a saline habitat in South Spain, showing lipolytic activity. METHODS AND FINDINGS: A lipolytic enzyme from the halophilic bacterium Marinobacter lipolyticus SM19 was isolated. This enzyme, designated LipBL, was expressed in Escherichia coli. LipBL is a protein of 404 amino acids with a molecular mass of 45.3 kDa and high identity to class C β-lactamases. LipBL was purified and biochemically characterized. The temperature for its maximal activity was 80°C and the pH optimum determined at 25°C was 7.0, showing optimal activity without sodium chloride, while maintaining 20% activity in a wide range of NaCl concentrations. This enzyme exhibited high activity against short-medium length acyl chain substrates, although it also hydrolyzes olive oil and fish oil. The fish oil hydrolysis using LipBL results in an enrichment of free eicosapentaenoic acid (EPA, but not docosahexaenoic acid (DHA, relative to its levels present in fish oil. For improving the stability and to be used in industrial processes LipBL was immobilized in different supports. The immobilized derivatives CNBr-activated Sepharose were highly selective towards the release of EPA versus DHA. The enzyme is also active towards different chiral and prochiral esters. Exposure of LipBL to buffer-solvent mixtures showed that the enzyme had remarkable activity and stability in all organic solvents tested. CONCLUSIONS: In this study we isolated, purified, biochemically characterized and immobilized a

  19. A high-fat diet delays age-related hearing loss progression in C57BL/6J mice.

    Directory of Open Access Journals (Sweden)

    Takeshi Fujita

    Full Text Available Age-related hearing loss (AHL, or presbycusis, is the most common sensory disorder among the elderly. We used C57BL/6J mice as an AHL model to determine a possible association between AHL and a high-fat diet (HFD.Forty C57BL/6J mice were randomly assigned to a control or HFD group. Each group was divided into the following subgroups: 1-, 3-, 5- and 12-month groups (HFD, n = 5/subgroup; control, n = 5/subgroup. Nine CBA/N-slc mice were also used as a 12-month control (n = 5 or 12-month HFD (n = 4 group. The mice were fed a HFD or normal (control diet throughout this study. Hearing function was evaluated at 1, 3, 5 and 12 months using auditory evoked brainstem responses (ABRs. Spiral ganglion cells (SGCs were also counted.The elevation of ABR thresholds (at 4 and 32 kHz at 3 and 5 months was significantly suppressed in the HFD group compared with the control groups for C57BL/6J mice. After 12 months, the elevation of ABR thresholds was significantly suppressed in the HFD group at all frequencies for C57BL/6J mice. In contrast, CBA/N-slc mice displayed opposite outcomes, as ABR thresholds at all frequencies at 12 months were significantly elevated in the HFD group compared with the control group. For the C57BL/6J mice at 12 months, SGC numbers significantly decreased in all parts of the cochleae in the control group compared with the HFD groups. In contrast, for the CBA/N-slc mice, SGC numbers significantly decreased, particularly in the upper parts of the cochleae in the HFD group compared with the control groups.The elevation in ABR thresholds and SGC loss associated with aging in the HFD-fed C57BL/6J mice were significantly suppressed compared with those in the normal diet-fed mice. These results suggest that HFD delays AHL progression in the C57B/6J mice.

  20. A High-Fat Diet Delays Age-Related Hearing Loss Progression in C57BL/6J Mice

    Science.gov (United States)

    Fujita, Takeshi; Yamashita, Daisuke; Uehara, Natsumi; Inokuchi, Go; Hasegawa, Shingo; Otsuki, Naoki; Nibu, Ken-ichi

    2015-01-01

    Objective Age-related hearing loss (AHL), or presbycusis, is the most common sensory disorder among the elderly. We used C57BL/6J mice as an AHL model to determine a possible association between AHL and a high-fat diet (HFD). Methods Forty C57BL/6J mice were randomly assigned to a control or HFD group. Each group was divided into the following subgroups: 1-, 3-, 5- and 12-month groups (HFD, n = 5/subgroup; control, n = 5/subgroup). Nine CBA/N-slc mice were also used as a 12-month control (n = 5) or 12-month HFD (n = 4) group. The mice were fed a HFD or normal (control) diet throughout this study. Hearing function was evaluated at 1, 3, 5 and 12 months using auditory evoked brainstem responses (ABRs). Spiral ganglion cells (SGCs) were also counted. Results The elevation of ABR thresholds (at 4 and 32 kHz) at 3 and 5 months was significantly suppressed in the HFD group compared with the control groups for C57BL/6J mice. After 12 months, the elevation of ABR thresholds was significantly suppressed in the HFD group at all frequencies for C57BL/6J mice. In contrast, CBA/N-slc mice displayed opposite outcomes, as ABR thresholds at all frequencies at 12 months were significantly elevated in the HFD group compared with the control group. For the C57BL/6J mice at 12 months, SGC numbers significantly decreased in all parts of the cochleae in the control group compared with the HFD groups. In contrast, for the CBA/N-slc mice, SGC numbers significantly decreased, particularly in the upper parts of the cochleae in the HFD group compared with the control groups. Conclusions The elevation in ABR thresholds and SGC loss associated with aging in the HFD-fed C57BL/6J mice were significantly suppressed compared with those in the normal diet-fed mice. These results suggest that HFD delays AHL progression in the C57B/6J mice. PMID:25625852

  1. Creating and Viewing HTML Files with Python

    Directory of Open Access Journals (Sweden)

    William J. Turkel

    2012-07-01

    Full Text Available This lesson uses Python to create and view an HTML file. If you write programs that output HTML, you can use any browser to look at your results. This is especially convenient if your program is automatically creating hyperlinks or graphic entities like charts and diagrams. Here you will learn how to create HTML files with Python scripts, and how to use Python to automatically open an HTML file in Firefox.

  2. Analyzing Android Browser Apps for file:// Vulnerabilities

    OpenAIRE

    Wu, Daoyuan; Chang, Rocky K. C.

    2014-01-01

    Securing browsers in mobile devices is very challenging, because these browser apps usually provide browsing services to other apps in the same device. A malicious app installed in a device can potentially obtain sensitive information through a browser app. In this paper, we identify four types of attacks in Android, collectively known as FileCross, that exploits the vulnerable file:// to obtain users' private files, such as cookies, bookmarks, and browsing histories. We design an automated s...

  3. Algorithms and file structures for computational geometry

    International Nuclear Information System (INIS)

    Hinrichs, K.; Nievergelt, J.

    1983-01-01

    Algorithms for solving geometric problems and file structures for storing large amounts of geometric data are of increasing importance in computer graphics and computer-aided design. As examples of recent progress in computational geometry, we explain plane-sweep algorithms, which solve various topological and geometric problems efficiently; and we present the grid file, an adaptable, symmetric multi-key file structure that provides efficient access to multi-dimensional data along any space dimension. (orig.)

  4. Creating and Viewing HTML Files with Python

    OpenAIRE

    William J. Turkel; Adam Crymble

    2012-01-01

    This lesson uses Python to create and view an HTML file. If you write programs that output HTML, you can use any browser to look at your results. This is especially convenient if your program is automatically creating hyperlinks or graphic entities like charts and diagrams. Here you will learn how to create HTML files with Python scripts, and how to use Python to automatically open an HTML file in Firefox.

  5. Evaluated nuclear-data file for niobium

    International Nuclear Information System (INIS)

    Smith, A.B.; Smith, D.L.; Howerton, R.J.

    1985-03-01

    A comprehensive evaluated nuclear-data file for elemental niobium is provided in the ENDF/B format. This file, extending over the energy range 10 -11 -20 MeV, is suitable for comprehensive neutronic calculations, particulary those dealing with fusion-energy systems. It also provides dosimetry information. Attention is given to the internal consistancy of the file, energy balance, and the quantitative specification of uncertainties. Comparisons are made with experimental data and previous evaluated files. The results of integral tests are described and remaining outstanding problem areas are cited. 107 refs

  6. 75 FR 24702 - Notice of Agreements Filed

    Science.gov (United States)

    2010-05-05

    ... Bay International Terminals, Inc. Operating Agreement. Parties: Tampa Bay International Terminals, Inc. and Tampa Port Authority. Filing Parties: Greg Lovelace, Director Cargo & Cruise Marketing; Tampa Port...

  7. Benchmark comparisons of evaluated nuclear data files

    International Nuclear Information System (INIS)

    Resler, D.A.; Howerton, R.J.; White, R.M.

    1994-05-01

    With the availability and maturity of several evaluated nuclear data files, it is timely to compare the results of integral tests with calculations using these different files. We discuss here our progress in making integral benchmark tests of the following nuclear data files: ENDL-94, ENDF/B-V and -VI, JENDL-3, JEF-2, and BROND-2. The methods used to process these evaluated libraries in a consistent way into applications files for use in Monte Carlo calculations is presented. Using these libraries, we are calculating and comparing to experiment k eff for 68 fast critical assemblies of 233,235 U and 239 Pu with reflectors of various material and thickness

  8. 76 FR 70651 - Fee for Filing a Patent Application Other Than by the Electronic Filing System

    Science.gov (United States)

    2011-11-15

    ... government; or (3) preempt tribal law. Therefore, a tribal summary impact statement is not required under... 0651-AC64 Fee for Filing a Patent Application Other Than by the Electronic Filing System AGENCY: United..., that is not filed by electronic means as prescribed by the Director of the United States Patent and...

  9. C57BL/6 mice need MHC class II Aq to develop collagen-induced arthritis dependent on autoreactive T cells.

    Science.gov (United States)

    Bäcklund, Johan; Li, Cuiqin; Jansson, Erik; Carlsen, Stefan; Merky, Patrick; Nandakumar, Kutty-Selva; Haag, Sabrina; Ytterberg, Jimmy; Zubarev, Roman A; Holmdahl, Rikard

    2013-07-01

    Collagen-induced arthritis (CIA) has traditionally been performed in MHC class II A(q)-expressing mice, whereas most genetically modified mice are on the C57BL/6 background (expressing the b haplotype of the major histocompatibility complex (MHC) class II region). However, C57BL/6 mice develop arthritis after immunisation with chicken-derived collagen type II (CII), but arthritis susceptibility has been variable, and the immune specificity has not been clarified. To establish a CIA model on the C57BL/6 background with a more predictable and defined immune response to CII. Both chicken and rat CII were arthritogenic in C57BL/6 mice provided they were introduced with high doses of Mycobacterium tuberculosis adjuvant. However, contaminating pepsin was strongly immunogenic and was essential for arthritis development. H-2(b)-restricted T cell epitopes on chicken or rat CII could not be identified, but expression of A(q) on the C57BL/6 background induced T cell response to the CII260-270 epitope, and also prolonged the arthritis to be more chronic. The putative (auto)antigen and its arthritogenic determinants in C57BL/6 mice remains undisclosed, questioning the value of the model for addressing T cell-driven pathological pathways in arthritis. To circumvent this impediment, we recommend MHC class II congenic C57BL/6N.Q mice, expressing A(q), with which T cell determinants have been thoroughly characterised.

  10. Cat odor exposure induces distinct changes in the exploratory behavior and Wfs1 gene expression in C57Bl/6 and 129Sv mice.

    Science.gov (United States)

    Raud, Sirli; Sütt, Silva; Plaas, Mario; Luuk, Hendrik; Innos, Jürgen; Philips, Mari-Anne; Kõks, Sulev; Vasar, Eero

    2007-10-16

    129Sv and C57Bl/6 (Bl6) strains are two most widely used inbred mice strains for generation of transgenic animals. The present study confirms the existence of substantial differences in the behavior of these two mice strains. The exploratory behavior of Bl6 mice in a novel environment was significantly higher compared to 129Sv mice. The exposure of mice to cat odor-induced an anxiety-like state in Bl6, but not in 129Sv mice. The levels of Wfs1 gene expression did not differ in the prefrontal cortex, mesolimbic area and temporal lobe of experimentally naive Bl6 and 129Sv mice. However, after cat odor exposure the expression of Wfs1 gene was significantly lower in the mesolimbic area and temporal lobe of Bl6 mice compared to 129Sv strain. Dynamics of Wfs1 gene expression and exploratory behavior suggest that the down-regulation of Wfs1 gene in Bl6 mice might be related to the increased anxiety. Further studies are needed to test the robustness and possible causal relationship of this finding.

  11. Acupuncture of Weizhong (BL 40) and Zusanli (ST 36) on the study of brain function by PET/CT imaging

    International Nuclear Information System (INIS)

    Shao Guangrui; Chen Ying; Yan Bin; Liu Cheng; Wang Guangcai; Tan Qiwen

    2006-01-01

    Objective: To explore the correlation between acupuncture of the points and certain functional areas of brain by PET/CT imaging. Methods: Twelve healthy volunteers were acupunctured separately in the point Weizhong (BL 40, right leg) and Zusanli(ST 36, right leg), and 5 consecutive PET/CT images were taken, statistical parameter map (SPM) paired t-test was analyzed between the different activated brain PET/CT imagings. Results: Changes of PET/CT imaging were found in acupuncture of the point Weizhong (BL 40) and Zusanli(ST 36) in 12 healthy volunteers. High metabolic areas were demonstrated in multiple brain regions, the data of two groups had significant difference between 2 points (t>4.03, P< 0.01). Conclusion: Acupuncturing the different point resulted activation of the glucose metabolism in different brain areas. (authors)

  12. Radix Stellariae extract prevents high-fat-diet-induced obesity in C57BL/6 mice by accelerating energy metabolism

    Directory of Open Access Journals (Sweden)

    Yin Li

    2017-05-01

    Full Text Available Stellaria dichotoma L. is widely distributed in Ningxia and surrounding areas in northwestern China. Its root, Radix Stellariae (RS, has been used in herbal formulae for treating asthenic-fever, infection, malaria, dyspepsia in children and several other symptoms. This study investigated whether the RS extract (RSE alleviates metabolic disorders. The results indicated that RSE significantly inhibited body weight gain in high-fat (HF-diet-fed C57BL/6 mice, reduced fasting glucose levels, and improved insulin tolerance. Moreover, RSE increased the body temperature of the mice and the expression of uncoupling proteins and peroxisome proliferator-activated receptors in the white adipose tissue. Thus, RSE alleviated metabolic disorders in HF-diet-fed C57BL/6 mice by potentially activating UCP and PPAR signaling.

  13. The effects of benzil amino purin (BAP and gibberellin with in vitro seedling growth of pulesari (Alyxia reinwardtii Bl

    Directory of Open Access Journals (Sweden)

    Heru Sudrajad

    2016-12-01

    Full Text Available Latar belakang: Status kelangkaan pulesari (Alyxia reinwardtii Bl. termasuk rawan (vulnerable karena tingginya permintaan dan mahalnya harga menyebabkan eksploitasi pulesari di hutan meningkat sedangkan upaya konservasi dan budidayanya belum ada. Selama ini perbanyakan pulesari hanya mengandalkan biji di alam. Benih pulesari persentase perkecambahannya tergolong sangat rendah, waktu lama dan sulit diperbanyak secara vegetatif. Memperhatikan sulitnya mendapatkan bibit pulesari secara konvensional maka perlu dilakukan dengan cara kultur jaringan. Metode: Penelitian dilakukan di laboratorium kultur jaringan Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional Tawangmangu selama tiga bulan. Bibit A. reinwardtii Bl. diperoleh dari Pringgondani Forest, Tawangmangu. Biji kupas dan dikeringkan selama lima hari, dicuci menggunakan aquadest steril, direndam pada 0,5% Agrept 5 menit kemudian pada 2,125% natrium hipochorida 5 menit dan dibilas menggunakan aquades steril. Media Murashige dan Skoog (MS disiapkan sesuai komposisi. Penelitian ini rancangan acak lengkap (RAL pola faktorial. Faktor pertama konsentrasi zat pengatur tumbuh BAP pada konsentrasi 3, 4 dan 5 mg/l dan faktor kedua konsentrasi giberelin dari 1, 2 dan 3 mg/l. Media MS yang dimodifikasi dengan penambahan BAP konsentrasi 3, 4, dan 5 mg/l dan giberelin konsentrasi 1, 2, dan 3 mg/l sehingga diperoleh 9 kombinasi perlakuan. Hasil: Hasil penelitian menunjukkan bahwa kombinasi perlakuan zat pengatur tumbuh BAP  mg/l dan giberelin 2 mg/l pada media MS terbentuk tunas dan akar. Tunas terbentuk pada 30 hari setelah tanam dengan tinggi 2 cm dan akar muncul pada 45 hari setelah tanam dalam satu kali percobaan. Kesimpulan: Pulesari (Alyxia reinwardtii Bl. dapat diperbanyak melalui kultur jaringan menggunakan biji sebagai eksplan. Kaca kunci : pulesari, Alyxia reinwardtii Bl, BAP dan giberelin Abstract Background: Alyxia reinwardtii Bl. in natural habitat becomes rare and reaches

  14. Utveckling av bestrykning för papper avsedda för bläckstråleskrivare

    OpenAIRE

    Mattsson, Ylva

    2002-01-01

    Detta examensarbete har utförts på papperstillverkaren Arjo Wiggins’ forskningscenter i Beaconsfield,England. Företaget har planer på att börja tillverka en ny papperskvalitet för bläckstråleskrivare, varförsyftet med arbetet har varit att testa och utvärdera olika sammansättningar av kemikalier som lämparsig för bestrykning av bläckstrålepapper.Ett stort antal bestrykningar har testats med varierande resultat. Det svåraste har visat sig vara attnå en tillräckligt hög grad av vattenhållfasthe...

  15. Effect of blocking TNF on IL-6 levels and metastasis in a B16-BL6 melanoma/mouse model.

    Science.gov (United States)

    Cubillos, S; Scallon, B; Feldmann, M; Taylor, P

    1997-01-01

    We studied the relationship between tumour necrosis factor (TNF) and interleukin 6 (IL-6) levels, and the metastatic process in C57BL/6 mice after intravenous inoculation of B16-BL6 melanoma cells. Bioactive TNF was not detectable in the sera of inoculated mice, but these animals did show higher TNF levels following intraperitoneal challenge with lipopolysaccharide (LPS) compared to control animals. Serum IL-6 levels were increased in inoculated animals. Injection of a hybrid molecule (p55-sf2) composed of the human p55 TNF receptor extracellular domain coupled to a human constant region backbone, decreased serum TNF (after LPS challenge) and IL-6 levels in inoculated animals. Lung metastases at 7-14 days were reduced, compared to human IgG-injected control animals, but this effect was lost at day 21 postinoculation. The results suggest that the reduction in the number of metastases may be related to the effect of blocking TNF activity.

  16. Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells.

    Science.gov (United States)

    Yan, C; Han, R

    1998-07-03

    Protein tyrosine phosphorylation occurs as one of the earlier events in cancer cell-extracellular matrix (ECM) interaction. With immunoblot analysis and immunofluorescence microscopy, genistein was found to suppress the tyrosine phosphorylation of proteins located at the cell periphery, including a 125 kDa protein, when B16-BL6 melanoma cells attached to and interacted with ECM. When accompanied by the suppression of adhesion-induced protein tyrosine phosphorylation, the invasive potential of B16-BL6 cells through reconstituted basement membrane was decreased significantly. However, neither adhesive capability nor cell growth was significantly affected by genistein. Therefore, the interruption of cancer cell-ECM interaction by suppression of protein tyrosine phosphorylation may contribute to invasion prevention of genistein.

  17. Growth inhibition and differentiation of murine melanoma B16-BL6 cells caused by the combination of cisplatin and caffeine.

    Science.gov (United States)

    Tsuchiya, H; Tomita, K; Yasutake, H; Ueda, Y; Tanaka, M; Sasaki, T

    1989-12-01

    We preliminarily investigated the combined effects of cisplatin and caffeine on murine melanoma B16-BL6 cells in vitro. When caffeine was added before or simultaneously with cisplatin, there was little growth inhibition. The addition of 2.0 mM caffeine after 1 h of exposure to cisplatin inhibited growth and induced cell differentiation. This treatment resulted in fewer cells, and the numbers of melanosomes and mitochondria and the amount of Golgi's complex and endoplasmic reticulum were increased. DNA histograms obtained by flow cytometry showed that cells treated with cisplatin alone accumulated in the G2/M phase, with a partial G2 block. The addition of 2.0 mM caffeine after 1 h of treatment with cisplatin reduced this block. Caffeine caused murine melanoma B16-BL6 cells treated with cisplatin to differentiate, and this inhibited growth.

  18. BL-7010 demonstrates specific binding to gliadin and reduces gluten-associated pathology in a chronic mouse model of gliadin sensitivity.

    Directory of Open Access Journals (Sweden)

    Justin L McCarville

    Full Text Available Celiac disease (CD is an autoimmune disorder in individuals that carry DQ2 or DQ8 MHC class II haplotypes, triggered by the ingestion of gluten. There is no current treatment other than a gluten-free diet (GFD. We have previously shown that the BL-7010 copolymer poly(hydroxyethyl methacrylate-co-styrene sulfonate (P(HEMA-co-SS binds with higher efficiency to gliadin than to other proteins present in the small intestine, ameliorating gliadin-induced pathology in the HLA-HCD4/DQ8 model of gluten sensitivity. The aim of this study was to investigate the efficiency of two batches of BL-7010 to interact with gliadin, essential vitamins and digestive enzymes not previously tested, and to assess the ability of the copolymer to reduce gluten-associated pathology using the NOD-DQ8 mouse model, which exhibits more significant small intestinal damage when challenged with gluten than HCD4/DQ8 mice. In addition, the safety and systemic exposure of BL-7010 was evaluated in vivo (in rats and in vitro (genetic toxicity studies. In vitro binding data showed that BL-7010 interacted with high affinity with gliadin and that BL-7010 had no interaction with the tested vitamins and digestive enzymes. BL-7010 was effective at preventing gluten-induced decreases in villus-to-crypt ratios, intraepithelial lymphocytosis and alterations in paracellular permeability and putative anion transporter-1 mRNA expression in the small intestine. In rats, BL-7010 was well-tolerated and safe following 14 days of daily repeated administration of 3000 mg/kg. BL-7010 did not exhibit any mutagenic effect in the genetic toxicity studies. Using complementary animal models and chronic gluten exposure the results demonstrate that administration of BL-7010 is effective and safe and that it is able to decrease pathology associated with gliadin sensitization warranting the progression to Phase I trials in humans.

  19. Affinity labelling in situ of the bL12 protein on E. coli 70S ribosomes by means of a tRNA dialdehyde derivative.

    Science.gov (United States)

    Hountondji, Codjo; Créchet, Jean-Bernard; Le Caër, Jean-Pierre; Lancelot, Véronique; Cognet, Jean A H; Baouz, Soria

    2017-12-01

    In this report, we have used periodate-oxidized tRNA (tRNAox) as an affinity laleling reagent to demonstrate that: (i) the bL12 protein contacts the CCA-arm of P-site bound tRNA on the Escherichia coli 70S ribosomes; (ii) the stoichiometry of labelling is one molecule of tRNAox bound to one polypeptide chain of endogenous bL12; (iii) cross-linking in situ of bL12 with tRNAox on the ribosomes provokes the loss of activity; (iv) intact tRNA protects bL12 in the 70S ribosomes against cross-linking with tRNAox; (v) both tRNAox and pyridoxal 5'-phosphate (PLP) compete for the same or for proximal cross-linking site(s) on bL12 inside the ribosome; (vi) the stoichiometry of cross-linking of PLP to the recombinant E. coli bL12 protein is one molecule of PLP covalently bound per polypeptide chain; (vii) the amino acid residue of recombinant bL12 cross-linked with PLP is Lys-65; (viii) Lys-65 of E. coli bL12 corresponds to Lys-53 of eL42 which was previously shown to cross-link with P-site bound tRNAox on human 80S ribosomes in situ; (ix) finally, E. coli bL12 and human eL42 proteins display significant primary structure similarities, which argues for evolutionary conservation of these two proteins located at the tRNA-CCA binding site on eubacterial and eukaryal ribosomes. © The Authors 2017. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

  20. 78 FR 70029 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-11-22

    ..., Ameren Energy Marketing Company, AmerenEnergy Resources Generating Company, AmerenEnergy Medina Valley... filing per 35: Integrated Marketplace Second Compliance Filing to be effective 3/ 1/2014. Filed Date: 11...

  1. 78 FR 32383 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-05-30

    .... Description: Inquiry Response to be effective 5/12/2013. Filed Date: 5/21/13. Accession Number: 20130521-5167....ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free...

  2. 77 FR 3758 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-01-25

    ...: Attachment H Schedule 7 Compliance Filing to be effective 6/1/2011. Filed Date: 1/13/12. Accession Number... filings: Docket Numbers: QF12-159-000. Applicants: City of Kinston, NC. Description: FERC Form 556 of City...

  3. 76 FR 55899 - Combined Notice of Filings No. 2

    Science.gov (United States)

    2011-09-09

    .... Applicants: Panther Interstate Pipeline Energy, LLC. Description: Panther Interstate Pipeline Energy, LLC submits tariff filing per 154.402: Panther 2011 ACA Filing to be effective 10/1/2011. Filed Date: 09/01...

  4. A basic evaluated neutronic data file for elemental scandium

    International Nuclear Information System (INIS)

    Smith, A.B.; Meadows, J.W.; Howerton, R.J.

    1992-01-01

    This report documents an evaluated neutronic data file for elemental scandium, presented in the ENDF/B-VI format. This file should provide basic nuclear data essential for neutronic calculations involving elemental scandium. No equivalent file was previously available

  5. 75 FR 49918 - Combined Notice of Filings # 1

    Science.gov (United States)

    2010-08-16

    ... Missouri Operations Company submits tariff filing per 35: KCP&L-GMO Baseline Compliance Filing to be.... Description: KCP&L Greater Missouri Operations Company submits tariff filing per 35.12: KCP&L-GMO OATT Volume...

  6. EPA FRS Facilities State Single File CSV Download

    Science.gov (United States)

    This page provides state comma separated value (CSV) files containing key information of all facilities and sites within the Facility Registry System (FRS). Each state zip file contains a single CSV file of key facility-level information.

  7. Effet de la substitution partielle de la farine de blé par la purée de ...

    African Journals Online (AJOL)

    La production de la pulpe séchée peut se faire à moindre coût. Mots clés : Substitution partielle, Farine de blé, Pulpe séchée, Purée de banane plantain Musa AAB, Gâteaux de plantain. Effect of partial substitution of wheat flour with mashed ripe plantain (Musa AAB) on pastry products quality. Objective: Plantain, third food ...

  8. Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway.

    Science.gov (United States)

    Dvorak, Pavel; Chrast, Lukas; Nikel, Pablo I; Fedr, Radek; Soucek, Karel; Sedlackova, Miroslava; Chaloupkova, Radka; de Lorenzo, Víctor; Prokop, Zbynek; Damborsky, Jiri

    2015-12-21

    Heterologous expression systems based on promoters inducible with isopropyl-β-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate LacI(Q)/P(lacUV5)-T7 vectors, are commonly used for production of recombinant proteins and metabolic pathways. The applicability of such cell factories is limited by the complex physiological burden imposed by overexpression of the exogenous genes during a bioprocess. This burden originates from a combination of stresses that may include competition for the expression machinery, side-reactions due to the activity of the recombinant proteins, or the toxicity of their substrates, products and intermediates. However, the physiological impact of IPTG-induced conditional expression on the recombinant host under such harsh conditions is often overlooked. The physiological responses to IPTG of the E. coli BL21(DE3) strain and three different recombinants carrying a synthetic metabolic pathway for biodegradation of the toxic anthropogenic pollutant 1,2,3-trichloropropane (TCP) were investigated using plating, flow cytometry, and electron microscopy. Collected data revealed unexpected negative synergistic effect of inducer of the expression system and toxic substrate resulting in pronounced physiological stress. Replacing IPTG with the natural sugar effector lactose greatly reduced such stress, demonstrating that the effect was due to the original inducer's chemical properties. IPTG is not an innocuous inducer; instead, it exacerbates the toxicity of haloalkane substrate and causes appreciable damage to the E. coli BL21(DE3) host, which is already bearing a metabolic burden due to its content of plasmids carrying the genes of the synthetic metabolic pathway. The concentration of IPTG can be effectively tuned to mitigate this negative effect. Importantly, we show that induction with lactose, the natural inducer of P lac , dramatically lightens the burden without reducing the efficiency of the synthetic TCP degradation

  9. Variability of the BL Lacertae objects PKS 2155 - 304 and OJ 287 in the far-ultraviolet

    International Nuclear Information System (INIS)

    Maraschi, L.; Treves, A.; Tagliaferri, G.; Tanzi, E.G.; CNR, Istituto di Fisica Cosmica, Milan, Italy)

    1986-01-01

    All the ultraviolet spectra of the two bright BL Lacertae objects PKS 2155 - 304 and OJ 287 taken with the International Ultraviolet Explorer in the period 1978-1984 are examined. For each spectrum the best-fitting power law is determined and a correlation between spectral slope and intensity is searched for. The correlation, if present, is weak. This is discussed in terms of models of the continuum emission of active galactic nuclei. 31 references

  10. Variability of the BL Lacertae objects PKS 2155 - 304 and OJ 287 in the far-ultraviolet

    Energy Technology Data Exchange (ETDEWEB)

    Maraschi, L.; Treves, A.; Tagliaferri, G.; Tanzi, E.G.

    1986-05-01

    All the ultraviolet spectra of the two bright BL Lacertae objects PKS 2155 - 304 and OJ 287 taken with the International Ultraviolet Explorer in the period 1978-1984 are examined. For each spectrum the best-fitting power law is determined and a correlation between spectral slope and intensity is searched for. The correlation, if present, is weak. This is discussed in terms of models of the continuum emission of active galactic nuclei. 31 references.

  11. Fertilité des sols agricoles sous vigne et sous blé de la région de ...

    African Journals Online (AJOL)

    L'objectif de ce travail est l'évaluation agronomique de la qualité du sol sous vigne et sous blé dans la région Mohammedia-Benslimane au Maroc, à travers les indicateurs chimiques de la qualité du sol (pH, CE, CaCO3, Carbone organique total, Azote, Phosphore et Potassium). Les résultats de cette étude montrent que ...

  12. An efficient chronic unpredictable stress protocol to induce stress-related responses in C57BL/6 mice

    Directory of Open Access Journals (Sweden)

    Susana eMonteiro

    2015-02-01

    Full Text Available Exposure to chronic stress can have broad effects on health ranging from increased predisposition for neuropsychiatric disorders to deregulation of immune responses. The chronic unpredictable stress (CUS protocol has been widely used to study the impact of stress exposure in several animal models and consists in the random, intermittent and unpredictable exposure to a variety of stressors during several weeks. CUS has consistently been shown to induce behavioral and immunological alterations typical of the chronic stress response. Unfortunately C57BL/6 mice, one of the most widely used mouse strains, due to the great variety of genetically modified lines, seem to be resistant to the commonly used 4-week-long CUS protocol. The definition of an alternative CUS protocol allowing the use of C57BL/6 mice in chronic stress experiments is a need. Here we show that by extending the CUS protocol to 8 weeks is possible to induce a chronic stress response in C57BL/6 mice, as revealed by abrogated body weight gain, increased adrenals weight and an overactive hypothalamic-pituitary-adrenal (HPA axis with increased levels of serum corticosterone. Moreover, we also observed stress-associated behavioral alterations, including the potentiation of anxious-like and depressive-like behaviors and a reduction of exploratory behavior, as well as subtle stress-related changes in the cell population of the thymus and of the spleen.The present protocol for C57BL/6 mice consistently triggers the spectrum of CUS-induced changes observed in rats and, thus, will be highly useful to researchers that need to use this particular mouse strain as an animal model of neuropsychiatric disorders and/or immune deregulation related to chronic unpredictable stress.

  13. Effet de la substitution partielle de la farine de blé par la purée de ...

    African Journals Online (AJOL)

    SARAH

    31 oct. 2014 ... de plantain bien mûr pourrait être recommandée aux industries locales dans la fabrication des produits de pâtisserie de haute qualité. La production de la pulpe séchée peut se faire à moindre coût. Mots clés : Substitution partielle, Farine de blé, Pulpe séchée, Purée de banane plantain Musa AAB,.

  14. An efficient chronic unpredictable stress protocol to induce stress-related responses in C57BL/6 mice.

    Science.gov (United States)

    Monteiro, Susana; Roque, Susana; de Sá-Calçada, Daniela; Sousa, Nuno; Correia-Neves, Margarida; Cerqueira, João José

    2015-01-01

    Exposure to chronic stress can have broad effects on health ranging from increased predisposition for neuropsychiatric disorders to deregulation of immune responses. The chronic unpredictable stress (CUS) protocol has been widely used to study the impact of stress exposure in several animal models and consists in the random, intermittent, and unpredictable exposure to a variety of stressors during several weeks. CUS has consistently been shown to induce behavioral and immunological alterations typical of the chronic stress-response. Unfortunately C57BL/6 mice, one of the most widely used mouse strains, due to the great variety of genetically modified lines, seem to be resistant to the commonly used 4-week-long CUS protocol. The definition of an alternative CUS protocol allowing the use of C57BL/6 mice in chronic stress experiments is a need. Here, we show that by extending the CUS protocol to 8 weeks is possible to induce a chronic stress-response in C57BL/6 mice, as revealed by abrogated body weight gain, increased adrenals weight, and an overactive hypothalamic-pituitary-adrenal axis with increased levels of serum corticosterone. Moreover, we also observed stress-associated behavioral alterations, including the potentiation of anxious-like and depressive-like behaviors and a reduction of exploratory behavior, as well as subtle stress-related changes in the cell population of the thymus and of the spleen. The present protocol for C57BL/6 mice consistently triggers the spectrum of CUS-induced changes observed in rats and, thus, will be highly useful to researchers that need to use this particular mouse strain as an animal model of neuropsychiatric disorders and/or immune deregulation related to CUS.

  15. Cannabidiol Prevents the Development of Cold and Mechanical Allodynia in Paclitaxel-Treated Female C57Bl6 Mice

    OpenAIRE

    Ward, Sara Jane; Ramirez, Michael David; Neelakantan, Harshini; Walker, Ellen Ann

    2011-01-01

    The taxane chemotherapeutic paclitaxel frequently produces peripheral neuropathy in humans. Rodent models to investigate mechanisms and treatments are largely restricted to male rats, whereas female mouse studies are lacking. We characterized a range of paclitaxel doses on cold and mechanical allodynia in male and female C57Bl/6 mice. Because the nonpsycho-active phytocannabinoid cannabidiol attenuates other forms of neuropathic pain, we assessed its effect on paclitaxel-induced allodynia. Pa...

  16. Probing BL Lac and Cluster Evolution via a Wide-angle, Deep X-ray Selected Sample

    Science.gov (United States)

    Perlman, E.; Jones, L.; White, N.; Angelini, L.; Giommi, P.; McHardy, I.; Wegner, G.

    1994-12-01

    The WARPS survey (Wide-Angle ROSAT Pointed Survey) has been constructed from the archive of all public ROSAT PSPC observations, and is a subset of the WGACAT catalog. WARPS will include a complete sample of >= 100 BL Lacs at F_x >= 10(-13) erg s(-1) cm(-2) . A second selection technique will identify ~ 100 clusters at 0.15 = 0.304 +/- 0.062 for XBLs but = 0.60 +/- 0.05 for RBLs. Models of the X-ray luminosity function (XLF) are also poorly constrained. WARPS will allow us to compute an accurate XLF, decreasing the error bars above by over a factor of two. We will also test for low-luminosity BL Lacs, whose non-thermal nuclear sources are dim compared to the host galaxy. Browne and Marcha (1993) claim the EMSS missed most of these objects and is incomplete. If their predictions are correct, 20-40% of the BL Lacs we find will fall in this category, enabling us to probe the evolution and internal workings of BL Lacs at lower luminosities than ever before. By removing likely QSOs before optical spectroscopy, WARPS requires only modest amounts of telescope time. It will extend measurement of the cluster XLF both to higher redshifts (z>0.5) and lower luminosities (LX<1x10(44) erg s(-1) ) than previous measurements, confirming or rejecting the 3sigma detection of negative evolution found in the EMSS, and constraining Cold Dark Matter cosmologies. Faint NELGs are a recently discovered major contributor to the X-ray background. They are a mixture of Sy2s, starbursts and galaxies of unknown type. Detailed classification and evolution of their XLF will be determined for the first time.

  17. BLENDED LEARNING (BL AS PEDAGOGICAL ALTERNATIVE TO TEACH BUSINESS COMMUNICATION COURSE: Case Study of UUM Executive Diploma Program

    Directory of Open Access Journals (Sweden)

    Hisham DZAKIRIA

    2012-07-01

    Full Text Available Globally, blended learning (BL technologies have been increasingly applied in a variety of fields, both public and private sectors. In recent years, universities, public and private businesses and organizations are among those employing blended learning methods and technologies in training and re-training of professionals in the workforce. In Malaysia, the increasing use of blended learning to enhance learning and enriching of soft skills among professionals and individuals in the work place is evident. The advancement of technology is an onset to many new avenues and tool for learning and teaching, and it is the coalescing of these various technologies with particular pedagogy or andragogy has helped to popularize BL. However, when an institution makes the critical choice of delivery methods, it is pertinent that the university needs to consider various success factors. One in particular is student-centered approach that entails the need to understand the students as the beneficiary of learning, and the support system they need to help them learn. This qualitative study reports in detail the experience of a small group of students undertaking Executive Diplomas at Executive Development Centre (EDC, Universiti Utara Malaysia as they progress through their Executive program. This paper looks at learning experiences as described by the learners- it is their story, their experience, and their perspective. This study suggests that BL offered a comfortable middle ground, and has lots of potential in higher education in Malaysia. It is a pedagogical alternative that could play a significant role not only for teaching Business Communication, but has the potential to promote lifelong learning initiatives in Malaysia in a much meaningful and inviting way. Although this study shows that BL contributed a significant and meaningful learning particularly for adult learners, it needs more definitive studies. Such information can be used to guide policy makers

  18. Effects of genistein, a soybean-derived isoflavone, on proliferation and differentiation of B16-BL6 mouse melanoma cells.

    Science.gov (United States)

    Yan, C H; Chen, X G; Li, Y; Han, R

    1999-01-01

    Genistein, a soybean-derived isoflavone, may contribute to the lower cancer incidence in South Asian countries. In this study, the effects and molecular mechanisms of genistein on growth and differentiation of B16-BL6 mouse melanoma cells were investigated. Genistein suppressed the growth of these melanoma cells. The IC50 value is 15.5 microM. On the other hand, genistein induced the changes of cell shape and cytoskeletal network. The cytoskeletal filaments were induced to form a bundle along the direction of elongation of the cells. Moreover, tyrosine phosphorylation levels of cytoskeleton-associated proteins decreased after the cells were exposed to 20 or 30 microM of genistein for 3 days. All these morphological and molecular changes were accompanied by appearance of the differentiated phenotypes. Genistein induced the increase of cellular melanin content, enhancement of tyrosinase activity, and decrease of colonization potentials in soft agar in a time-dependent and dose-dependent manner. The effective concentration was no more than 10 microM after 3 days' exposure. The tumorigenic potentials of B16-BL6 cells in C57BL/6 mouse also decreased after exposure to 20 or 30 microM of genistein for 3 days. When expressions of tumor-related genes were investigated in the differentiation-induced cells, the content of P53 dramatically increased while that of c-Myc protein decreased. Therefore, due to its ability to induce cellular and molecular changes, genistein suppressed the growth and induced differentiated phenotypes in B16-BL6 melanoma cells.

  19. Fungiform Taste Bud Degeneration in C57BL/6J Mice Following Chorda-Lingual Nerve Transection

    OpenAIRE

    Guagliardo, Nick A.; Hill, David L.

    2007-01-01

    Taste buds are dependent on innervation for normal morphology and function. Fungiform taste bud degeneration after chorda tympani nerve injury has been well documented in rats, hamsters, and gerbils. The current study examines fungiform taste bud distribution and structure in adult C57BL/6J mice from both intact taste systems and after unilateral chorda-lingual nerve transection. Fungiform taste buds were visualized and measured with the aid of cytokeratin 8. In control mice, taste buds were ...

  20. High School and Beyond: Twins and Siblings' File Users' Manual, User's Manual for Teacher Comment File, Friends File Users' Manual.

    Science.gov (United States)

    National Center for Education Statistics (ED), Washington, DC.

    These three users' manuals are for specific files of the High School and Beyond Study, a national longitudinal study of high school sophomores and seniors in 1980. The three files are computerized databases that are available on magnetic tape. As one component of base year data collection, information identifying twins, triplets, and some non-twin…

  1. Collagen-induced arthritis in C57BL/6 mice is associated with a robust and sustained T-cell response to type II collagen

    OpenAIRE

    Inglis, Julia J; Criado, Gabriel; Medghalchi, Mino; Andrews, Melanie; Sandison, Ann; Feldmann, Marc; Williams, Richard O

    2007-01-01

    Many genetically modified mouse strains are now available on a C57BL/6 (H-2b) background, a strain that is relatively resistant to collagen-induced arthritis. To facilitate the molecular understanding of autoimmune arthritis, we characterised the induction of arthritis in C57BL/6 mice and then validated the disease as a relevant pre-clinical model for rheumatoid arthritis. C57BL/6 mice were immunised with type II collagen using different protocols, and arthritis incidence, severity, and respo...

  2. 76 FR 28018 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-05-13

    ... tariff filing per 35.13(a)(2)(iii: Information Policy Revisions to be effective 6/20/ 2011. Filed Date... Interconnection, L.L.C. Description: PJM Interconnection, L.L.C. submits tariff filing per 35.13(a)(2)(iii: Queue... New Mexico submits tariff filing per 35.13(a)(2)(iii: PNM LGIP Filing to be effective 7/5/2011. Filed...

  3. 75 FR 62381 - Combined Notice of Filings #2

    Science.gov (United States)

    2010-10-08

    ... filing per 35.12: MeadWestvaco Virginia MBR Filing to be effective 9/ 28/2010. Filed Date: 09/29/2010... submits tariff filing per 35.12: City Power MBR Tariff to be effective 9/30/2010. Filed Date: 09/29/2010... Baseline MBR Tariff to be effective 9[sol]29[sol]2010. Filed Date: 09/29/2010. Accession Number: 20100929...

  4. Environmental Baseline File National Transportation

    International Nuclear Information System (INIS)

    Harris, M.

    1999-01-01

    This Environmental Baseline File summarizes and consolidates information related to the national-level transportation of commercial spent nuclear fuel. Topics addressed include: shipments of commercial spent nuclear fuel based on mostly truck and mostly rail shipping scenarios; transportation routing for commercial spent nuclear fuel sites and DOE sites; radionuclide inventories for various shipping container capacities; transportation routing; populations along transportation routes; urbanized area population densities; the impacts of historical, reasonably foreseeable, and general transportation; state-level food transfer factors; Federal Guidance Report No. 11 and 12 radionuclide dose conversion factors; and national average atmospheric conditions

  5. Complex discriminative stimulus properties of (+)lysergic acid diethylamide (LSD) in C57Bl/6J mice.

    Science.gov (United States)

    Benneyworth, Michael A; Smith, Randy L; Barrett, Robert J; Sanders-Bush, Elaine

    2005-06-01

    The drug discrimination procedure is the most frequently used in vivo model of hallucinogen activity. Historically, most drug discrimination studies have been conducted in the rat. With the development of genetically modified mice, a powerful new tool has become available for investigating the mechanisms of drug-induced behavior. The current paper is part of an ongoing effort to determine the utility of the drug discrimination technique for evaluating hallucinogenic drugs in mice. To establish the training procedures and characterize the stimulus properties of (+)lysergic acid diethylamide (LSD) in mice. Using a two-lever drug discrimination procedure, C57Bl/6J mice were trained to discriminate 0.45 mg/kg LSD vs saline on a VI30 sec schedule of reinforcement, with vanilla-flavored Ensure serving as the reinforcer. As in rats, acquisition was orderly, but the training dose was nearly five-fold higher for mice than rats. LSD lever selection was dose-dependent. Time-course studies revealed a rapid loss of the LSD stimulus effects. The 5-HT(2A/2C) receptor agonist, 2,5-dimethoxy-4-bromoamphetamine [(-)DOB] (1.0 mg/kg), substituted fully for LSD and the 5-HT(1A) receptor agonist, 8-hydroxy-2-(di-n-propylamino)-tetralin (8-OH-DPAT) (1.6 mg/kg), substituted partially for LSD. Pretreatment with the 5-HT(2A) receptor-selective antagonist, MDL 100907, or the 5-HT(1A)-selective antagonist WAY 100635, showed that each antagonist only partially blocked LSD discrimination. Substitution of 1.0 mg/kg (-)DOB for LSD was fully blocked by pretreatment with MDL 100907 but unaltered by WAY 100635 pretreatment. These data suggest that in mice the stimulus effects of LSD have both a 5-HT(2A) receptor and a 5-HT(1A) receptor component.

  6. Aqueous stability and oral pharmacokinetics of meloxicam and carprofen in male C57BL/6 mice.

    Science.gov (United States)

    Ingrao, Joelle C; Johnson, Ron; Tor, Elizabeth; Gu, Yu; Litman, Marcus; Turner, Patricia V

    2013-09-01

    We found that carprofen and meloxicam under 3 environmental conditions (ambient dark, ambient light, and 4 °C) remained stable for at least 7 d. We then evaluated the oral pharmacokinetics of meloxicam (20 mg/kg) and carprofen (10 mg/kg) in male C57BL/6 mice after oral gavage or administration in the drinking water. Mice did not drink meloxicam-medicated water but readily consumed carprofen-medicated water, consuming an average of 14.19 mL carprofen-medicated water per 100 g body weight daily; mice drank more during the dark phase than during the light phase. Plasma analyzed by HPLC (meloxicam) and tandem mass spectrometry (carprofen) revealed that the peak meloxicam and carprofen concentrations were 16.7 and 20.3 μg/mL and occurred at 4 and 2 h after oral gavage, respectively. Similar blood levels were achieved after 12 h access to the carprofen-medicated water bottle. At 24 h after oral gavage, the drugs were not detectable in plasma. Meloxicam plasma AUC, elimination half-life, apparent volume of distribution, and apparent oral clearance were 160.4 mg/L × h, 7.4 h, 0.36 L/kg, and 0.125 mL/h × kg, respectively. Carprofen plasma AUC, elimination half-life, apparent volume of distribution, and apparent oral clearance were 160.8 mg/L × h, 7.4 h, 0.42 L/kg, and 0.062 mL/h × kg, respectively. No gross or microscopic evidence of toxicity was seen in any mouse. Our findings indicate that carprofen can be administered in drinking water to mice and that medicated water bottles should be placed 12 to 24 h prior to painful procedures.

  7. Inhibition of phosphodiesterase 4 reduces ethanol intake and preference in C57BL/6J mice

    Directory of Open Access Journals (Sweden)

    Yuri A. Blednov

    2014-05-01

    Full Text Available Some anti-inflammatory medications reduce alcohol consumption in rodent models. Inhibition of phosphodiesterases (PDE increases cAMP and reduces inflammatory signaling. Rolipram, an inhibitor of PDE4, markedly reduced ethanol intake and preference in mice and reduced ethanol seeking and consumption in alcohol-preferring fawn-hooded rats (Hu et al., 2011;Wen et al., 2012. To determine if these effects were specific for PDE4, we compared nine PDE inhibitors with different subtype selectivity: propentofylline (nonspecific, vinpocetine (PDE1, olprinone, milrinone (PDE3, zaprinast (PDE5, rolipram, mesopram, piclamilast, and CDP840 (PDE4. Alcohol intake was measured in C57BL/6J male mice using 24-hour two-bottle choice and two-bottle choice with limited (three-hour access to alcohol. Only the selective PDE4 inhibitors reduced ethanol intake and preference in the 24-hour two-bottle choice test. For rolipram, piclamilast, and CDP840, this effect was observed after the first 6 hours but not after the next 18 hours. Mesopram, however, produced a long-lasting reduction of ethanol intake and preference. In the limited access test, rolipram, piclamilast, and mesopram reduced ethanol consumption and total fluid intake and did not change preference for ethanol, whereas CDP840 reduced both consumption and preference without altering total fluid intake. Our results provide novel evidence for a selective role of PDE4 in regulating ethanol drinking in mice. We suggest that inhibition of PDE4 may be an unexplored target for medication development to reduce excessive alcohol consumption.

  8. Response of C57Bl/6 mice to a carbohydrate-free diet

    Directory of Open Access Journals (Sweden)

    Borghjid Saihan

    2012-07-01

    Full Text Available Abstract High fat feeding in rodents generally leads to obesity and insulin resistance whereas in humans this is only seen if dietary carbohydrate is also high, the result of the anabolic effect of poor regulation of glucose and insulin. A previous study of C57Bl/6 mice (Kennedy AR, et al.: Am J Physiol Endocrinol Metab (2007 262 E1724-1739 appeared to show the kind of beneficial effects of calorie restriction that is seen in humans but that diet was unusually low in protein (5%. In the current study, we tested a zero-carbohydrate diet that had a higher protein content (20%. Mice on the zero-carbohydrate diet, despite similar caloric intake, consistently gained more weight than animals consuming standard chow, attaining a dramatic difference by week 16 (46.1 ± 1.38 g vs. 30.4 ± 1.00 g for the chow group. Consistent with the obese phenotype, experimental mice had fatty livers and hearts as well as large fat deposits in the abdomino-pelvic cavity, and showed impaired glucose clearance after intraperitoneal injection. In sum, the response of mice to a carbohydrate-free diet was greater weight gain and metabolic disruptions in distinction to the response in humans where low carbohydrate diets cause greater weight loss than isocaloric controls. The results suggest that rodent models of obesity may be most valuable in the understanding of how metabolic mechanisms can work in ways different from the effect in humans.

  9. Effects of ethanol on offspring of C57BL/6J mice alcoholized during gestation

    Directory of Open Access Journals (Sweden)

    Grinfeld Hermann

    1999-01-01

    Full Text Available The effects of chronic alcohol consumption during pregnancy were analysed in the gestation and offspring of alcoholized mice. Female C57BL/6J mice were placed overnight with stud males and the presence of a sperm plug in the next morning indicated the onset of gestation. Pregnant mice were distributed in two weight-matched groups. In the alcoholized group, the mice received a high protein liquid diet ad libitum containing 27.5% of ethanol-derived calories (5.28% v/v from gestation day 5 to 19. The control group received the same volume of diet containing isocaloric amounts of maltose-dextrin substituted for ethanol. After postnatal day zero, the dams received food pellets and tap water ad libitum. On postnatal day 6 the pups were counted and weighed at variable intervals up to the 60th day of life. The majority of the pregnant dams that have received ethanol completed the gestational period, and the chronic consumption of alcohol did not interfere with the number of dams that gave birth. The alcoholized and control dams gained an equivalent weight and consumed an equivalent volume of diet throughout the gestation. The number of pups from alcohol diet dams was 46,26% smaller compared with the control group. There were less male than female pups in the offspring of alcoholized mice. Teratogeny like gastroschisis and limb malformation were present in the offspring of alcoholized dams. The body weight of the offspring of alcoholized mice increased from the 18th to the 36th postnatal day.

  10. Environmental Enrichment Blunts Ethanol Consumption after Restraint Stress in C57BL/6 Mice.

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    Priscila Marianno

    Full Text Available Elevated alcohol intake after abstinence is a key feature of the addiction process. Some studies have shown that environmental enrichment (EE affects ethanol intake and other reinforcing effects. However, different EE protocols may vary in their ability to influence alcohol consumption and stress-induced intake. The present study evaluated whether short (3 h or continuous (24 h EE protocols affect ethanol consumption after periods of withdrawal. Mice were challenged with stressful stimuli (24 h isolation and restraint stress to evaluate the effects of stress on drinking. Male C57BL/6 mice were subjected to a two-bottle choice drinking-in-the-dark paradigm for 15 days (20% ethanol and water, 2 h/day, acquisition phase. Control mice were housed under standard conditions (SC. In the first experiment, one group of mice was housed under EE conditions 24 h/day (EE24h. In the second experiment, the exposure to EE was reduced to 3 h/day (EE3h. After the acquisition phase, the animals were deprived of ethanol for 6 days, followed by 2 h ethanol access once a week. Animals were tested in the elevated plus maze (EPM during ethanol withdrawal. During the last 2 weeks, the mice were exposed to 24 h ethanol access. A 1-h restraint stress test was performed immediately before the last ethanol exposure. EE24h but not EE3h increased anxiety-like behavior during withdrawal compared to controls. Neither EE24h nor EE3h affected ethanol consumption during the 2 h weekly exposure periods. However, EE24h and EE3h mice that were exposed to acute restraint stress consumed less ethanol than controls during a 24 h ethanol access. These results showed that EE reduces alcohol intake after an acute restraint stress.

  11. A multi-mineral natural product inhibits liver tumor formation in C57BL/6 mice.

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    Aslam, Muhammad N; Bergin, Ingrid; Naik, Madhav; Hampton, Anna; Allen, Ron; Kunkel, Steven L; Rush, Howard; Varani, James

    2012-06-01

    C57BL/6 mice were maintained for up to 18 months on high-fat and low-fat diets with or without a multi-mineral supplement derived from the skeletal remains of the red marine algae Lithothamnion calcareum. Numerous grossly observable liver masses were visible in animals on the "western-style" high-fat diet sacrificed at 12 and 18 months. The majority of the masses were in male mice (20 out of 100 males versus 3 out of 100 females; p = 0.0002). There were more liver masses in animals on the high-fat diet than on the low-fat diet (15 out of 50 on high-fat versus 5 out of 50 on low-fat; p = 0.0254). The multi-mineral supplement reduced the number of liver masses in mice on both diets (3 out of 25 male mice in the low-fat diet group without the supplement versus 1 out of 25 mice with supplement; 12 of 25 male mice in the high-fat diet group without the supplement versus 3 of 25 mice with supplement [p = 0.0129]). Histological evaluation revealed a total of 17 neoplastic lesions (9 adenomas and 8 hepatocellular carcinomas), and 18 pre-neoplastic lesions. Out of eight hepatocellular carcinomas, seven were found in unsupplemented diet groups. Steatosis was widely observed in livers with and without grossly observable masses, but the multi-mineral supplement had no effect on the incidence of steatosis or its severity. Taken together, these findings suggest that a multi-mineral-rich natural product can protect mice against neoplastic and pre-neoplastic proliferative liver lesions that may develop in the face of steatosis.

  12. Vaccination evokes gender-dependent protection against tularemia infection in C57BL/6Tac mice.

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    Sunagar, Raju; Kumar, Sudeep; Franz, Brian J; Gosselin, Edmund J

    2016-06-17

    Francisella tularensis (Ft) is a Category A biothreat agent for which there currently is no FDA-approved vaccine. Thus, there is a substantial effort underway to develop an effective tularemia vaccine. While it is well established that gender can significantly impact susceptibility to primary infection, the impact of gender on vaccine efficacy is not well established. Thus, development of a successful vaccine against tularemia will require an understanding of the impact gender has on vaccine-induced protection against this organism. In this study, a role for gender in vaccine-induced protection following Ft challenge is identified for the first time. In the present study, mucosal vaccination with inactivated Ft (iFt) LVS elicited gender-based protection in C57BL/6Tac mice against respiratory challenge with Ft LVS. Specifically, vaccinated male mice were more susceptible to subsequent Ft LVS challenge. This increased susceptibility in male mice correlated with increased bacterial burden, increased tissue inflammation, and increased proinflammatory cytokine production late in post-challenge infection. In contrast, improved survival of iFt-vaccinated female mice correlated with reduced bacterial burden and enhanced levels of Ft-specific Abs in serum and broncho-alveolar lavage (BAL) fluid post-challenge. Furthermore, vaccination with a live attenuated vaccine consisting of an Ft LVS superoxide dismutase (SodB) mutant, which has proven efficacious against the highly virulent Ft SchuS4 strain, demonstrated similar gender bias in protection post-Ft SchuS4 challenge. Of particular significance is the fact that these are the first studies to demonstrate that gender differences impact disease outcome in the case of lethal respiratory tularemia following mucosal vaccination. In addition, these studies further emphasize the fact that gender differences must be a serious consideration in any future tularemia vaccine development studies. Copyright © 2016 Elsevier Ltd. All

  13. Caloric restriction in C57BL/6J mice mimics therapeutic fasting in humans

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    Denny Christine A

    2006-05-01

    Full Text Available Abstract Background Caloric restriction (CR has long been recognized as a dietary therapy that improves health and increases longevity. Little is known about the persistent effects of CR on plasma biomarkers (glucose, ketone bodies, and lipids following re-feeding in mice. It is also unclear how these biomarker changes in calorically restricted mice relate to those observed previously in calorically restricted humans. Results Three groups of individually housed adult female C57BL/6J (B6 mice (n = 4/group were fed a standard rodent chow diet either: (1 unrestricted (UR; (2 restricted for three weeks to reduce body weight by approximately 15–20% (R; or (3 restricted for three weeks and then re-fed unrestricted (ad libitum for an additional three weeks (R-RF. Body weight and food intake were measured throughout the study, while plasma lipids and levels of glucose and ketone bodies (β-hydroxybutyrate were measured at the termination of the study. Plasma glucose, phosphatidylcholine, cholesterol, and triglycerides were significantly lower in the R mice than in the UR mice. In contrast, plasma fatty acids and β-hydroxybutyrate were significantly higher in the R mice than in the UR mice. CR had no effect on plasma phosphatidylinositol levels. While body weight and plasma lipids of the R-RF mice returned to unrestricted levels upon re-feeding, food intake and glucose levels remained significantly lower than those prior to the initiation of CR. Conclusion CR establishes a new homeostatic state in B6 mice that persists for at least three weeks following ad libitum re-feeding. Moreover, the plasma biomarker changes observed in B6 mice during CR mimic those reported in humans on very low calorie diets or during therapeutic fasting.

  14. Tier-2 studies on monocrotaline immunotoxicity in C57BL/6 mice.

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    Deyo, J A; Kerkvliet, N I

    1991-01-01

    Monocrotaline (MCT) is a member of a class of naturally occurring phytotoxins known as pyrrolizidine alkaloids, and is a toxicological concern to both man and his livestock. The purpose of these studies was to evaluate the effect of a 14-day oral MCT (0-100 mg/kg per day) exposure on the functional integrity of various immunocyte effector systems in C57BL/6 mice, as well as to investigate potential mechanisms for its immunotoxicity. Decreases in lymphoid organ weights and cellularity, and resident peritoneal exudate cell (PEC) number were only observed after exposure to the highest dose of 100 mg/kg MCT. This dose also inhibited NK cell cytotoxicity, while the total number of NK lytic units per spleen was decreased (-53%) after exposure to 50 mg/kg MCT. Following i.p. injection of SRBC, the percentage of PEC macrophages containing engulfed SRBC was significantly increased in MCT-exposed mice, while the percentage of large vacuolated (activated) macrophages was decreased in a dose-dependent manner. Exposure to MCT significantly decreased the total number of Ig+ cells without altering the number of CD4+ and CD8+ cells. The antibody responses (PFC/10(6) spleen cells) to two T cell-independent antigens, TNP-LPS and DNP-Ficoll, were significantly decreased at all MCT doses, and the degree of suppression of both responses was identical at coincident doses. MCT exposure (25 mg/kg) significantly suppressed the blastogenic response to the T cell mitogen concanavalin A (-38%), and to the B cell mitogen lipopolysaccharide (-58%). These results indicate that exposure to MCT can alter the functional integrity of various immune effector responses in a dose-dependent manner, and suggest that the B cell may be a relatively more sensitive target of MCT immunotoxicity compared to T cells, macrophages and NK cells.

  15. Quinine controls body weight gain without affecting food intake in male C57BL6 mice

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    Cettour-Rose Philippe

    2013-02-01

    Full Text Available Abstract Background Quinine is a natural molecule commonly used as a flavouring agent in tonic water. Diet supplementation with quinine leads to decreased body weight and food intake in rats. Quinine is an in vitro inhibitor of Trpm5, a cation channel expressed in taste bud cells, the gastrointestinal tract and pancreas. The objective of this work is to determine the effect of diet supplementation with quinine on body weight and body composition in male mice, to investigate its mechanism of action, and whether the effect is mediated through Trpm5. Results Compared with mice consuming AIN, a regular balanced diet, mice consuming AIN diet supplemented with 0.1% quinine gained less weight (2.89 ± 0.30 g vs 5.39 ± 0.50 g and less fat mass (2.22 ± 0.26 g vs 4.33 ± 0.43 g after 13 weeks of diet, and had lower blood glucose and plasma triglycerides. There was no difference in food intake between the mice consuming quinine supplemented diet and those consuming control diet. Trpm5 knockout mice gained less fat mass than wild-type mice. There was a trend for a diet-genotype interaction for body weight and body weight gain, with the effect of quinine less pronounced in the Trpm5 KO than in the WT background. Faecal weight, energy and lipid contents were higher in quinine fed mice compared to regular AIN fed mice and in Trpm5 KO mice compared to wild type mice. Conclusion Quinine contributes to weight control in male C57BL6 mice without affecting food intake. A partial contribution of Trpm5 to quinine dependent body weight control is suggested.

  16. Six-year optical monitoring of the BL Lacertae object 1ES 0806+52.4

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    Man, Zhongyi; Zhang, Xiaoyuan; Wu, Jianghua [Department of Astronomy, Beijing Normal University, Beijing 100875 (China); Zhou, Xu [Key Laboratory of Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, 20A Datun Road, Beijing 100012 (China); Yuan, Qirong, E-mail: jhwu@bnu.edu.cn [Department of Physics and Institute of Theoretical Physics, Nanjing Normal University, Nanjing 210046 (China)

    2014-12-01

    We present the results of the first systematic long-term multicolor optical monitoring of the BL Lacertae object 1ES 0806+52.4. The monitoring was performed in multiple passbands with a 60/90 cm Schmidt telescope from 2005 December to 2011 February. The overall brightness of this object decreased from 2005 December to 2008 December but was regained after that. A sharp outburst probably occurred around the end of our monitoring program. Overlapping the long-term trend are some short-term small-amplitude oscillations. No intranight variability was found in the object, which is in accordance with the historical observations before 2005. By investigating the color behavior, we found a strong bluer-when-brighter chromatism for the long-term variability of 1ES 0806+52.4. The total amplitudes at the c, i, and o bands are 1.18, 1.12, and 1.02 mag, respectively. The amplitudes tend to increase toward shorter wavelengths, which may be a major cause of the bluer-when-brighter chromatism. Such bluer-when-brighter chromatisms are also found in other blazars, such as S5 0716+714, OJ 287. The hard-X-ray data collected from the Swift/BAT archive was correlated with our optical data. No positive result was found, the reason for which may be that the hard-X-ray flux is a combination of the synchrotron and inverse Compton emission, but with different timescales and cadences under the leptonic synchrotron self-Compton model.

  17. Cadmium Exposure Impairs Cognition and Olfactory Memory in Male C57BL/6 Mice.

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    Wang, Hao; Zhang, Liang; Abel, Glen M; Storm, Daniel R; Xia, Zhengui

    2018-01-01

    Cadmium (Cd) is a heavy metal of high interest to the superfund initiative. Recent epidemiology studies have suggested a possible association between Cd exposure and cognitive as well as olfactory impairments in humans. However, studies in animal models are needed to establish a direct causal relationship between Cd exposure and impairments in cognition and olfaction. This study aims to investigate the toxic effect of Cd on cognition and olfactory function in mice. One group of 8-week-old C57BL/6 male mice was exposed to 3 mg/l Cd (in the form of CdCl2) through drinking water for 20 weeks for behavior tests and final blood Cd concentration analysis. The behavior tests were conducted before, during, and after Cd exposure to analyze the effects of Cd on cognition and olfactory function. Upon completion of behavior tests, blood was collected to measure final blood Cd concentration. Two additional groups of mice were similarly exposed to Cd for 5 or 13 weeks for peak blood Cd concentration measurement. The peak blood Cd concentration was 2.125-2.25 μg/l whereas the final blood Cd concentration was 0.18 μg/l. At this exposure level, Cd impaired hippocampus-dependent learning and memory in novel object location test, T-maze test, and contextual fear memory test. It also caused deficits in short-term olfactory memory and odor-cued olfactory learning and memory. Results in this study demonstrate a direct relationship between Cd exposure and cognitive as well as olfactory impairments in an animal model. © The Author 2017. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  18. Protein Oxidation in the Lungs of C57BL/6J Mice Following X-Irradiation

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    Barshishat-Kupper, Michal; McCart, Elizabeth A.; Freedy, James G.; Tipton, Ashlee J.; Nagy, Vitaly; Kim, Sung-Yop; Landauer, Michael R.; Mueller, Gregory P.; Day, Regina M.

    2015-01-01

    Damage to normal lung tissue is a limiting factor when ionizing radiation is used in clinical applications. In addition, radiation pneumonitis and fibrosis are a major cause of mortality following accidental radiation exposure in humans. Although clinical symptoms may not develop for months after radiation exposure, immediate events induced by radiation are believed to generate molecular and cellular cascades that proceed during a clinical latent period. Oxidative damage to DNA is considered a primary cause of radiation injury to cells. DNA can be repaired by highly efficient mechanisms while repair of oxidized proteins is limited. Oxidized proteins are often destined for degradation. We examined protein oxidation following 17 Gy (0.6 Gy/min) thoracic X-irradiation in C57BL/6J mice. Seventeen Gy thoracic irradiation resulted in 100% mortality of mice within 127–189 days postirradiation. Necropsy findings indicated that pneumonitis and pulmonary fibrosis were the leading cause of mortality. We investigated the oxidation of lung proteins at 24 h postirradiation following 17 Gy thoracic irradiation using 2-D gel electrophoresis and OxyBlot for the detection of protein carbonylation. Seven carbonylated proteins were identified using mass spectrometry: serum albumin, selenium binding protein-1, alpha antitrypsin, cytoplasmic actin-1, carbonic anhydrase-2, peroxiredoxin-6, and apolipoprotein A1. The carbonylation status of carbonic anhydrase-2, selenium binding protein, and peroxiredoxin-6 was higher in control lung tissue. Apolipoprotein A1 and serum albumin carbonylation were increased following X-irradiation, as confirmed by OxyBlot immunoprecipitation and Western blotting. Our findings indicate that the profile of specific protein oxidation in the lung is altered following radiation exposure. PMID:28248270

  19. Inhibition of IKKβ Reduces Ethanol Consumption in C57BL/6J Mice.

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    Truitt, Jay M; Blednov, Yuri A; Benavidez, Jillian M; Black, Mendy; Ponomareva, Olga; Law, Jade; Merriman, Morgan; Horani, Sami; Jameson, Kelly; Lasek, Amy W; Harris, R Adron; Mayfield, R Dayne

    2016-01-01

    Proinflammatory pathways in neuronal and non-neuronal cells are implicated in the acute and chronic effects of alcohol exposure in animal models and humans. The nuclear factor-κB (NF-κB) family of DNA transcription factors plays important roles in inflammatory diseases. The kinase IKKβ mediates the phosphorylation and subsequent proteasomal degradation of cytosolic protein inhibitors of NF-κB, leading to activation of NF-κB. The role of IKKβ as a potential regulator of excessive alcohol drinking had not previously been investigated. Based on previous findings that the overactivation of innate immune/inflammatory signaling promotes ethanol consumption, we hypothesized that inhibiting IKKβ would limit/decrease drinking by preventing the activation of NF-κB. We studied the systemic effects of two pharmacological inhibitors of IKKβ, TPCA-1 and sulfasalazine, on ethanol intake using continuous- and limited-access, two-bottle choice drinking tests in C57BL/6J mice. In both tests, TPCA-1 and sulfasalazine reduced ethanol intake and preference without changing total fluid intake or sweet taste preference. A virus expressing Cre recombinase was injected into the nucleus accumbens and central amygdala to selectively knock down IKKβ in mice genetically engineered with a conditional Ikkb deletion ( Ikkb F/F ). Although IKKβ was inhibited to some extent in astrocytes and microglia, neurons were a primary cellular target. Deletion of IKKβ in either brain region reduced ethanol intake and preference in the continuous access two-bottle choice test without altering the preference for sucrose. Pharmacological and genetic inhibition of IKKβ decreased voluntary ethanol consumption, providing initial support for IKKβ as a potential therapeutic target for alcohol abuse.

  20. Magnolia Extract (BL153 Ameliorates Kidney Damage in a High Fat Diet-Induced Obesity Mouse Model

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    Wenpeng Cui

    2013-01-01

    Full Text Available Accumulating evidence demonstrated that obesity is a risk factor for renal structural and functional changes, leading to the end-stage renal disease which imposes a heavy economic burden on the community. However, no effective therapeutic method for obesity-associated kidney disease is available. In the present study, we explored the therapeutic potential of a magnolia extract (BL153 for treating obesity-associated kidney damage in a high fat diet- (HFD- induced mouse model. The results showed that inflammation markers (tumor necrosis factor-α and plasminogen activator inhibitor-1 and oxidative stress markers (3-nitrotyrosine and 4-hydroxy-2-nonenal were all significantly increased in the kidney of HFD-fed mice compared to mice fed with a low fat diet (LFD. Additionally, proteinuria and renal structure changes in HFD-fed mice were much more severe than that in LFD-fed mice. However, all these alterations were attenuated by BL153 treatment, accompanied by upregulation of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α and hexokinase II (HK II expression in the kidney. The present study indicates that BL153 administration may be a novel approach for renoprotection in obese individuals by antiinflammation and anti-oxidative stress most likely via upregulation of PGC-1α and HK II signal in the kidney.