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Sample records for fibrinolytic activity fa

  1. Fibrinolytic activity in Nigerian diabetics

    OpenAIRE

    Adediran, I; Ikem, R; Borisade, M

    2004-01-01

    Fibrinolytic activity, using euglobulin lysis time (ELT), was assessed in 46 Nigerians with type 2 diabetes mellitus to study the effect of the disease on fibrinolytic component of haemostasis. There were 20 females and 26 males. Fifty age matched non-diabetics and apparently healthy Nigerians were similarly studied as controls; there were 24 females and 26 males. In the patients, the mean (SD) age of the females was 56.7 (12.0) years and mean (SD) ELT was 276.4 (62.2) min; the mean age of th...

  2. Activity assessment of microbial fibrinolytic enzymes.

    Science.gov (United States)

    Kotb, Essam

    2013-08-01

    Conversion of fibrinogen to fibrin inside blood vessels results in thrombosis, leading to myocardial infarction and other cardiovascular diseases. In general, there are four therapy options: surgical operation, intake of antiplatelets, anticoagulants, or fibrinolytic enzymes. Microbial fibrinolytic enzymes have attracted much more attention than typical thrombolytic agents because of the expensive prices and the side effects of the latter. The fibrinolytic enzymes were successively discovered from different microorganisms, the most important among which is the genus Bacillus. Microbial fibrinolytic enzymes, especially those from food-grade microorganisms, have the potential to be developed as functional food additives and drugs to prevent or cure thrombosis and other related diseases. There are several assay methods for these enzymes; this may due to the insolubility of substrate, fibrin. Existing assay methods can be divided into three major groups. The first group consists of assay of fibrinolytic activity with natural proteins as substrates, e.g., fibrin plate methods. The second and third groups of assays are suitable for kinetic studies and are based on the determination of hydrolysis of synthetic peptide esters. This review will deal primarily with the microorganisms that have been reported in literature to produce fibrinolytic enzymes and the first review discussing the methods used to assay the fibrinolytic activity.

  3. The fibrinolytic response in injured animal tissues normally low in fibrinolytic activity

    NARCIS (Netherlands)

    Smokovitis, A.; Astrup, T.

    1980-01-01

    Rabbit skin, normally of low fibrinolytic activity, and guinea-pig skin, of medium activity, after crush injury or after the intradermal or subcutaneous injection of aluminum hydroxide, show a remarkable increase in fibrinolytic activity. The activity is caused by a plasminogen activator and is

  4. Screening for fibrinolytic activity in eight Viperid venoms.

    Science.gov (United States)

    Ramírez, M S; Sánchez, E E; García-Prieto, C; Pérez, J C; Chapa, G R; McKeller, M R; Ramírez, R; De Anda, Y

    1999-09-01

    Snake venoms contain direct-acting fibrinolytic metalloproteinases (MMP) that could have important applications in medicine. Fibrinolytic enzymes isolated from venom can induce in vitro clot lysis by directly acting on a fibrin clot. The most ideal fibrinolytic enzyme would have high affinity for clots, dissolve clots directly without causing hemorrhage, and would not be neutralized in vivo by endogenous metalloproteinase inhibitors. The purpose of this study was to compare DEAE/HPLC venom profiles from Viperid snakes and identify fractions that contain fibrinolytic activity with no hemorrhagic activity and are not neutralized by animal sera. The sera selected were from four (Virginia opossum, Gray woodrat, Mexican ground squirrel, and Hispid cottonrat) animals known to neutralize hemorrhagic activity in snake venoms. Nineteen fractions from the Viperid venoms had fibrinolytic activity. Agkistrodon venom fractions contained the highest specific fibrinolytic activities. A. piscivorus leucostoma fraction 4 contained a high specific fibrinolytic activity, no hemorrhagic activity, and the fibrinolytic activity was not neutralized by the proteinase inhibitors of the four animal sera. A. contortrix laticinctus fraction 1 also had a high specific fibrinolytic activity and no hemorrhagic activity. However, the fibrinolytic activity was neutralized by Didelphis virginiana (Virginia opossum) serum.

  5. The influence of lipoprotein(a) on fibrinolytic activity

    OpenAIRE

    Rahajuningsih D. Setiabudy; Marzuki Suryaatmadja; Fifi Henrika

    2004-01-01

    Lipoprotein(a) is a plasma lipoprotein whose structure and composition are similar with low density lipoprotein (LDL) with an addition of apo(a) that is bound to apo B100. The structure of apo(a) is similar with plasminogen, a proenzym in fibrinolytic system. Due to this similarity, it is assumed that Lp(a) can inhibit plasminogen activity and decreases fibrinolytic activity. The purpose of this study is to prove that addition of Lp(a) to normal plasma can inhibit fibrinolytic activity. Four ...

  6. The influence of lipoprotein(a on fibrinolytic activity

    Directory of Open Access Journals (Sweden)

    Rahajuningsih D. Setiabudy

    2004-09-01

    Full Text Available Lipoprotein(a is a plasma lipoprotein whose structure and composition are similar with low density lipoprotein (LDL with an addition of apo(a that is bound to apo B100. The structure of apo(a is similar with plasminogen, a proenzym in fibrinolytic system. Due to this similarity, it is assumed that Lp(a can inhibit plasminogen activity and decreases fibrinolytic activity. The purpose of this study is to prove that addition of Lp(a to normal plasma can inhibit fibrinolytic activity. Four healthy people whose fibrinogen levels, plasminogen activities and euglobulin clot lysis time were within normal range were enrolled in this study. Fibrinolytic activity were assessed by euglobulin clot lysis time (ECLT. In the first experiment, the addition of Lp(a was done before centrifugation to obtain euglobulin precipitates, while in the second experiment, Lp(a was added to the euglobulin precipitates. As a control, ECLT was performed in the plasma with the addition of NaCl 0.9% in the same volume with Lp(a. The results of the study showed that in the first experiment, there was no clot formation. It is assumed that Lp(a can bind fibrinogen and both of them floated in the supernatant, so there was no fibrinogen in the euglobulin precipitate that can be clotted by thrombin. In the second experiment, the clot did not dissolve until the fourth day. In conclusion, the addition of Lp(a to normal plasma can inhibit the activity of fibrinolytic system. (Med J Indones 2004; 13: 135-9 Keywords: plasminogen, fibrinogen, apo(a, euglobulin clot lysis time (ECLT

  7. Impaired fibrinolytic activity is present in children with dyslipidemias

    NARCIS (Netherlands)

    Albisetti, M; Chan, AKC; McCrindle, BW; Wong, D; Monagle, P; Andrew, M

    2004-01-01

    Dyslipidemias are major risk factors for atherosclerosis and cardiovascular disease. Abnormalities of fibrinolytic and coagulation components are considered useful predictors of cardiovascular morbidity and mortality in adults. This study examined whether fibrinolytic and coagulation components are

  8. Effect of supine exercise on platelet aggregation and fibrinolytic activity

    DEFF Research Database (Denmark)

    Dag, B; Fornitz, Gitte Gleerup; Bak, A M

    1994-01-01

    In 12 healthy young men, strenuous cycling exercise in the supine position, caused platelet aggregability to decrease and the ADP threshold to rise from 7.0 microM resting, to 9.5 exercising (P ... from 178 to 68 min, PAI-1 fell from 8.91 to 5.16 IU ml-1, and t-PA rose from 0.56 to 3.95 IU ml-1, all three values were significant to P exercise, it did not increase platelet activity as expected, but caused a modest increase...... of fibrinolytic activity. These results suggest that supine exercise will not affect the haemostatic system adversely....

  9. Fibrinolytic and coagulative activities of Yersinia pestis

    Directory of Open Access Journals (Sweden)

    Timo K. Korhonen

    2013-07-01

    Full Text Available The outer membrane protease Pla belongs to the omptin protease family spread by horizontal gene transfer into Gram-negative bacteria that infect animals or plants. Pla has adapted to support the life style of the plague bacterium Yersinia pestis. Pla has a -barrel fold with 10 membrane-spanning  strands and five surface loops, and the barrel surface contains bound lipopolysaccharide (LPS that is critical for the conformation and the activity of Pla. The biological activity of Pla is influenced by the structure of the surface loops around the active site groove and by temperature-induced LPS modifications. Several of the putative virulence-related functions documented for Pla in vitro address control of the human hemostatic system, i.e. coagulation and fibrinolysis. Pla activates human plasminogen to the serine protease plasmin and activates the physiological plasminogen activator urokinase. Pla also inactivates the protease inhibitors alpha-2-antiplasmin and plasminogen activator inhibitor 1 and prevents the activation of thrombin-activatable fibrinolysis inhibitor. These functions enhance uncontrolled fibrinolysis which is thought to improve Y. pestis dissemination and survival in the mammalian host, and lowered fibrin(ogen deposition has indeed been observed in mice infected with Pla-positive Y. pestis. However, Pla also inactivates an anticoagulant, the tissue factor pathway inhibitor, which should increase fibrin formation and clotting. Thus Pla and Y. pestis have complex interactions with the hemostatic system. Y. pestis modifies its LPS upon transfer to the mammalian host and we hypothesize that the contrasting biological activities of Pla in coagulation and fibrinolysis are influenced by LPS changes during infection.

  10. Fibrinolytic changes in pregnant women on highly active antiretroviral therapy.

    Science.gov (United States)

    Osime, Odaburhine E; Ese-Onakewhor, Joseph U; Kolade, Samson O

    2015-02-01

    To report on the changes in fibrinolytic activity in human immunodeficiency virus (HIV) infected pregnant women who are undergoing highly active antiretroviral therapy (HAART). Blood was collected from 50 HIV positive women on HAART (test subjects), and 50 HIV positive women not on HAART (controls). These women were attending the prevention of mother to child clinic (PMTCT) of the University of Benin Teaching Hospital, Benin City, Nigeria from January to June 2014. Standard manual techniques were used to estimate plasma fibrinogen concentration (PFC), euglobulin lysis time (ELT), packed cell volume (PCV), and plasma viscosity (PV). The mean ± standard error of mean (SEM) of PFC was 4.02±0.13 g/l and ELT from the test subjects was 378±15 mins was significantly higher (p0.05). There were differences in the various parameters investigated when the various trimesters were compared. These differences did not, however, follow a particular pattern. Highly active antiretroviral therapy can cause changes in fibrinolytic activity that may predispose pregnant women to hyperfibrinogenemia and anemia.

  11. FIBRINOLYTIC-ACTIVITY IN THE ABDOMINAL-CAVITY OF RATS WITH FECAL PERITONITIS

    NARCIS (Netherlands)

    van Goor, Harry; de Graaf, JS; Sluiter, WJ; van der Meer, J; Bom, VJJ; Bleichrodt, RP

    1994-01-01

    Generalized peritonitis causes a reduction in abdominal fibrinolytic activity, resulting in persistence of intraabdominal fibrin with subsequent adhesion and abscess formation. The activities of tissue plasminogen activator (tPA) and plasminogen activator inhibitor (PAI) were measured in the periton

  12. FIBRINOLYTIC-ACTIVITY IN THE ABDOMINAL-CAVITY OF RATS WITH FECAL PERITONITIS

    NARCIS (Netherlands)

    van Goor, Harry; de Graaf, JS; Sluiter, WJ; van der Meer, J; Bom, VJJ; Bleichrodt, RP

    1994-01-01

    Generalized peritonitis causes a reduction in abdominal fibrinolytic activity, resulting in persistence of intraabdominal fibrin with subsequent adhesion and abscess formation. The activities of tissue plasminogen activator (tPA) and plasminogen activator inhibitor (PAI) were measured in the periton

  13. Platelet function and fibrinolytic activity following distance running.

    Science.gov (United States)

    Knudsen, J B; Brodthagen, U; Gormsen, J; Jordal, R; Nørregaard-Hansen, K; Paulev, P E

    1982-11-01

    6 long distance runners from the Danish marathon elite and 6 non-runners completed test runs of 28 and 12 km, respectively. Distance runners and non-runners showed the same responses in platelet function. We found a significant decrease in ADP induced platelet aggregability, a decreased serotonin release induced by ADP and collagen and an increase in platelet factor 4 immediately following the run. The antithrombin III levels remained constant. Euglobulin lysis time was shortened (by approximately 50%) and the plasminogen levels significantly increased. The last 2 findings indicate an equal increase in fibrinolytic activity during distance running in both groups. While short term, strenuous exercise induces platelet hyperaggregation, long term distance running induces a state of exhaustion of platelet aggregation capacity.

  14. Lipids, lipoproteins, fibrinogen and fibrinolytic activity in angiographically assessed coronary heart disease.

    Science.gov (United States)

    Lipinska, I; Gurewich, V; Meriam, C M; Kosowsky, B D; Ramaswamy, K; Philbin, E; Losordo, D

    1987-01-01

    Plasma lipids, lipoproteins, fibrinogen and fibrinolytic activity (FA) were measured in 202 consecutive patients undergoing coronary angiography. Twenty-one patients, 13 men and 8 women with a mean age of 52.8 years and 56.7 years respectively, were found to be angiographically free of coronary artery disease (CAD) and served as the principal control group. Since this group contained a disproportionate number of subjects with risk factors such as family history, hypertension and smoking, a second control group of clinically healthy subjects selected for age was also tested. Their laboratory results were not used in the statistical calculations. The group with angiographic CAD consisted of 130 men (mean age 57.6 years) and 51 women (mean age 61.5 years). Abnormal angiograms were graded according to the number of major vessels with more than 50% stenosis involved. The laboratory variables which were significantly (p less than .01-.001) associated with the presence of CAD were: High density lipoprotein (HDL) when determined by polyacrylamide gel electrophoresis (PAGE) and expressed as a percentage of total lipoproteins rather than concentration, presence of Intermediate Density Lipoprotein (IDL), percent of Very Low Density Lipoprotein (VLDL), fibrinogen concentration and FA. The HDL2 subfraction was significantly inversely correlated only in women. The total plasma cholesterol was normal and virtually identical in both groups. Within the CAD group, only two of the laboratory results were significantly correlated with the extent of disease. By univariate analysis, the FA showed the closest association with the score for severity of CAD (p less than .001) followed by the presence of IDL (p less than .01). In conclusion, lipoprotein analysis by a method which measures not only HDL, but also LDL, VLDL and IDL, together with the determination of fibrinogen and FA provides information useful in the identification of individuals at risk for CAD.

  15. Fibrinolytic activity of kaempferol isolated from the fruits of Lagenaria siceraria (Molina) Standley.

    Science.gov (United States)

    Rajput, M S; Mathur, Vineet; Agrawal, Purti; Chandrawanshi, H K; Pilaniya, Urmila

    2011-11-01

    This study was undertaken to isolate a flavonol, kaempferol, from the fruits of Lagenaria siceraria (bottle gourd) as a sole compound and to explore the fibrinolytic potential of the methanolic extract of the fruits of L. siceraria and the isolated compound using their in vitro activity. The fibrinolytic activity in terms of percentage of plasma clot liquefaction was determined by plasma clot lysis at 37°C in 24 h. The fibrinolytic activity of both substances was compared to the well-known thrombolytic agent streptokinase (30,000 IU). The percentage of fibrinolytic activity of the extract and isolated compound were found to be 54.72 ± 0.7210 and 77.37 ± 1.3010, respectively. Streptokinase was considered as the standard fibrinolytic enzyme for comparative purposes and had 91.46 ± 0.7625% fibrinolytic activity. The conclusion drawn in our study after testing the hypothesis by experimental procedures is that in vitro fibrinolytic activity on plasma clots is an inherent property of kaempferol isolated from the fruits of L. siceraria, and its comparison with streptokinase is a new aspect for further study.

  16. Asbestos exposure increases human bronchial epithelial cell fibrinolytic activity.

    Science.gov (United States)

    Gross, T J; Cobb, S M; Gruenert, D C; Peterson, M W

    1993-03-01

    Chronic exposure to asbestos fibers results in fibrotic lung disease. The distal pulmonary epithelium is an early target of asbestos-mediated injury. Local plasmin activity may be important in modulating endoluminal inflammatory responses in the lung. We studied the effects of asbestos exposure on cell-mediated plasma clot lysis as a marker of pericellular plasminogen activation. Exposing human bronchial epithelial (HBE) cells to 100 micrograms/ml of asbestos fibers for 24 h resulted in increased plasma clot lysis. Fibrinolytic activity was augmented in a dose-dependent fashion, was not due to secreted protease, and occurred only when there was direct contact between the plasma clot and the epithelial monolayer. Further analysis showed that asbestos exposure increased HBE cell-associated urokinase-type plasminogen activator (uPA) activity in a time-dependent manner. The increased cell-associated PA activity could be removed by acid washing. The increase in PA activity following asbestos exposure required new protein synthesis because it was abrogated by treatment with either cycloheximide or actinomycin D. Therefore, asbestos exposure increases epithelial-mediated fibrinolysis by augmenting expression of uPA activity at the cell surface by mechanisms that require new RNA and protein synthesis. These observations suggest a novel mechanism whereby exposure of the distal epithelium to inhaled particulates may result in a chronic inflammatory response that culminates in the development of fibrotic lung disease.

  17. Fibrinolytic Activity of Blood and its Determinants in Healthy Medical Students

    Science.gov (United States)

    Shoeb, M.; Bose, S.

    2015-01-01

    Background Decreased fibrinolytic activity results in longer fibrinolysis time of blood and an increased propensity for hypercoagulable states of blood. Subjects with less fibrinolytic activity are prone to coronary artery disease, stroke and thromboembolic phenomena. Aim The study aims to identify the impact of gender, dietary habits, body mass index, physical activity level and menstrual cycle on fibrinolytic activity in healthy subjects. Settings and Design Cross-sectional study on randomly selected 206 healthy medical students aged 17 to 23 years. Materials and Methods Anthropometric measurements, dietary habits, physical activity level and menstrual history were obtained. Fibrinolysis time of fasting venous blood sample was determined by Euglobulin Lysis Time (ELT) method. Results Highly significant gender difference was noted in mean fibrinolytic activity (p=0.002). Mean fibrinolytic activity also showed a significant relationship with BMI (p=0.001) and with different phases of menstrual cycle in females (p=0.004). However, such relationship was not observed with diet and physical activity (p>0.05) in boys and girls. Conclusion Gender difference, body mass index and menstrual cycle phases have influences on the fibrinolytic activity of blood. This might be due to cyclical changes in the sex hormones levels, endometrium-derived plasmin and plasminogen activators and excess production of plasminogen activator inhibitor type-1 (PAI-I) in visceral adipocytes. PMID:26266114

  18. FA

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — An Area Forecast (FA) is a forecast of Visual Flight Rules (VFR) clouds and weather conditions over an area as large as the size of several states.

  19. PROTEOLYTIC AND FIBRINOLYTIC ACTIVITIES OF HALOPHILIC LACTIC ACID BACTERIA FROM TWO INDONESIAN FERMENTED FOODS

    Directory of Open Access Journals (Sweden)

    Asep A. Prihanto

    2013-04-01

    Full Text Available Exploration of fermented foods as sources of fibrinolytic enzymes is increased in the last decades. Terasi and Jambal roti is Indonesian traditional fermented fish products, which were famous in Java Island. Both are important products in Indonesian dishes, especially in Java. Investigation on halophilic lactic acid bacteria using MRS and M-17 agar obtained seventy four isolated strains. Their proteolytic and fibrinolytic activities were determined using skim milk agar and plasminogen-free fibrin plate. Twenty five isolates showed protease activities, while only four of them secreted fibrinolitic enzyme. The highest proteolytic and fibrinolytic activity was shown by TB1 strain, which is identified as Bacillus coagulans. The 16s rDNA is still in investigating to confirm the TB1 strain identity.

  20. Change in fibrinolytic activity under the influence of a constant magnetic field. [blood coagulation normilization in heart patients

    Science.gov (United States)

    Yepishina, S. G.

    1974-01-01

    The fibrinolytic activity of plasma changes under the influence of a constant magnetic field (CMF) with a strength of 250 or 2500 oersteds. CMF shows a tendency toward normalization of fibrinolytic processes in the presence of pathological disturbances in fibrinolysis activation.

  1. The effect of chemical anti-inhibitors on fibrinolytic enzymes and inhibitors

    DEFF Research Database (Denmark)

    Sidelmann, Johannes Jakobsen; Jespersen, J; Kluft, C;

    1997-01-01

    Fibrinolytic enzyme inhibitors hamper the determination of the specific fibrinolytic serine protease activity. Reportedly, chemical anti-inhibitors eliminate the influence of fibrinolytic inhibitors, but it remains unclear to what extent they change the specific activity of fibrinolytic serine pr...

  2. Fibrinolytic and procoagulant activities of Yersinia pestis and Salmonella enterica.

    Science.gov (United States)

    Korhonen, T K

    2015-06-01

    Pla of the plague bacterium Yersinia pestis and PgtE of the enteropathogen Salmonella enterica are surface-exposed, transmembrane β-barrel proteases of the omptin family that exhibit a complex array of interactions with the hemostatic systems in vitro, and both proteases are established virulence factors. Pla favors fibrinolysis by direct activation of plasminogen, inactivation of the serpins plasminogen activator inhibitor-1 and α2-antiplasmin, inactivation of the thrombin-activable fibrinolysis inhibitor, and activation of single-chain urokinase. PgtE is structurally very similar but exhibits partially different functions and differ in expression control. PgtE proteolysis targets control aspects of fibrinolysis, and mimicry of matrix metalloproteinases enhances cell migration that should favor the intracellular spread of the bacterium. Enzymatic activity of both proteases is strongly influenced by the environment-induced variations in lipopolysaccharide that binds to the β-barrel. Both proteases cleave the tissue factor pathway inhibitor and thus also express procoagulant activity. © 2015 International Society on Thrombosis and Haemostasis.

  3. [Effects of cholesterol rich diet on blood coagulative and fibrinolytic activities in male rabbits].

    Science.gov (United States)

    Si, Quan-jin; Li, Xiao-ying

    2005-05-01

    To explore the effects of cholesterol rich diet on the activities of blood coagulative and fibrinolytic systems in male rabbits. 14 male New Zealand white rabbits were randomized to cholesterol rich diet(CRD) group and common diet (control) group. Rabbits in CRD group were fed with 1% cholesterol embedded diet and those in the control group were fed with common diet. Levels of blood TG, TC, LDL, HDL, Lp(a), apoA1, apoB, FIB, D-dimers and FDP, PT and APTT, activity of ADP, AT-III, PLG and alpha2-PI were tested in all rabbits before given cholesterol rich diet and after 12 weeks' feeding with different kinds of diet. Levels of blood TG, TC, LDL, HDL, Lp(a), apoA1, apoB, FIB, D-dimers in CRD group were all elevated significantly compared with those in the control group and the baseline levels. PT and APTT were shortened, ADP, PLG and alpha2-PI activity were increased in CRD group. Cholesterol rich diet not only is the direct cause of hyperlipidemia but also can increase the coagulative activity and inhibit the fibrinolytic activity and promoting the evolution of arteriosclerosis.

  4. Cloning, Expression and Activity Analysis of a Novel Fibrinolytic Serine Protease fromArenicola cristata

    Institute of Scientific and Technical Information of China (English)

    ZHAO Chunling; JU Jiyu

    2015-01-01

    The full-length cDNA of a protease gene from a marine annelid Arenicola cristata was amplified through rapid amplifi-cation of cDNA ends technique and sequenced. The size of the cDNA was 936 bp in length, including an open reading frame encod-ing a polypeptide of 270 amino acid residues. The deduced amino acid sequnce consisted of pro- and mature sequences. The protease belonged to the serine protease family because it contained the highly conserved sequence GDSGGP. This protease was novel as it showed a low amino acid sequence similarity (<40%) to other serine proteases. The gene encoding the active form ofA. cristata serine protease was cloned and expressed inE. coli. Purified recombinant protease in a supernatant could dissolve an artificial fibrin plate with plasminogen-rich fibrin, whereas the plasminogen-free fibrin showed no clear zone caused by hydrolysis. This result sug-gested that the recombinant protease showed an indirect fibrinolytic activity of dissolving fibrin, and was probably a plasminogen activator. A rat model with venous thrombosis was established to demonstrate that the recombinant protease could also hydrolyze blood clotin vivo. Therefore, this recombinant protease may be used as a thrombolytic agent for thrombosis treatment. To our knowledge, this study is the first of reporting the fibrinolytic serine protease gene inA. cristata.

  5. Cloning, expression and activity analysis of a novel fibrinolytic serine protease from Arenicola cristata

    Science.gov (United States)

    Zhao, Chunling; Ju, Jiyu

    2015-06-01

    The full-length cDNA of a protease gene from a marine annelid Arenicola cristata was amplified through rapid amplification of cDNA ends technique and sequenced. The size of the cDNA was 936 bp in length, including an open reading frame encoding a polypeptide of 270 amino acid residues. The deduced amino acid sequnce consisted of pro- and mature sequences. The protease belonged to the serine protease family because it contained the highly conserved sequence GDSGGP. This protease was novel as it showed a low amino acid sequence similarity (< 40%) to other serine proteases. The gene encoding the active form of A. cristata serine protease was cloned and expressed in E. coli. Purified recombinant protease in a supernatant could dissolve an artificial fibrin plate with plasminogen-rich fibrin, whereas the plasminogen-free fibrin showed no clear zone caused by hydrolysis. This result suggested that the recombinant protease showed an indirect fibrinolytic activity of dissolving fibrin, and was probably a plasminogen activator. A rat model with venous thrombosis was established to demonstrate that the recombinant protease could also hydrolyze blood clot in vivo. Therefore, this recombinant protease may be used as a thrombolytic agent for thrombosis treatment. To our knowledge, this study is the first of reporting the fibrinolytic serine protease gene in A. cristata.

  6. Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays

    Directory of Open Access Journals (Sweden)

    Abuzar Elnager

    2015-01-01

    Full Text Available Background. Caffeic acid phenethyl ester (CAPE has been reported to possess time-dependent fibrinolytic activity by in vitro assay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE using in vitro assays. Methods. Standardized human whole blood (WB clots were incubated in either blank controls or different concentrations of CAPE (3.75, 7.50, 15.00, 22.50, and 30.00 mM. After 3 hours, D-dimer (DD levels and WB clot weights were measured for each concentration. Thromboelastography (TEG parameters were recorded following CAPE incubation, and fibrin morphology was examined under a confocal microscope. Results. Overall, mean DD (μg/mL levels were significantly different across samples incubated with different CAPE concentrations, and the median pre- and postincubation WB clot weights (grams were significantly decreased for each CAPE concentration. Fibrin removal was observed microscopically and indicated dose-dependent effects. Based on the TEG test, the Ly30 fibrinolytic parameter was significantly different between samples incubated with two different CAPE concentrations (15.0 and 22.50 mM. The 50% effective dose (ED50 of CAPE (based on DD was 1.99 mg/mL. Conclusions. This study suggests that CAPE possesses fibrinolytic activity following in vitro incubation and that it has dose-dependent activities. Therefore, further investigation into CAPE as a potential alternative thrombolytic agent should be conducted.

  7. Blood Fibrinolytic Activity in Patients with Type 2 Diabetes Mellitus Associated with Hypothyroidism

    Directory of Open Access Journals (Sweden)

    Т.Yu. Yuzvenko

    2015-06-01

    Full Text Available The objective of the research was to study the level of plasminogen activator inhibitor type 1 (PAI-1 in the blood serum of patients with type 2 diabetes mellitus (DM in combination with hypothyroidism. We have observed 62 patients with type 2 DM, 32 of them had primary hypothyroidism. Control group consisted of 20 persons without carbohydrate metabolism disorders and thyroid pathology. In patients with type 2 DM, we have detected violations in the fibrinolytic system as an increase of PAI-1, whose level depends on the body mass index, waist circumference, level of glycated haemoglobin, indexes of insulin resistance, blood lipid spectrum, as well as on the presence of microalbuminuria. The presence of hypothyroidism has an additional negative impact on the parameters of carbohydrate, lipid metabolism and PAI-1 level in patients with type 2 DM.

  8. A fibrinolytic protease AfeE from Streptomyces sp. CC5, with potent thrombolytic activity in a mouse model.

    Science.gov (United States)

    Sun, Zhibin; Liu, Pingping; Cheng, Guangyan; Zhang, Biying; Dong, Weiliang; Su, Xingli; Huang, Yan; Cui, Zhongli; Kong, Yi

    2016-04-01

    Fibrinolytic proteases have potential applications in cardiovascular disease therapy. A novel fibrinolytic protease, AfeE, with strong thrombolytic activity was purified from Streptomyces sp. CC5. AfeE displayed maximum activity at 40°C in the pH range of 7.0-12.0. It was strongly inhibited by serine protease inhibitor phenylmethanesulfonylfluoride, soybean trypsin inhibitor, tosyl-l-lysine chloromethyl ketone and tosyl-l-phenylalanine chloromethyl ketone. The activity of the enzyme was partially inhibited by Cu(2+), Co(2+) and Zn(2+). AfeE exhibited higher substrate specificity for fibrin than fibrinogen, which has rarely been reported in fibrinolytic enzymes. AfeE also showed high thrombolytic activity in a carrageenan-induced mouse tail thrombosis model. AfeE prolonged prothrombin time, activated partial thromboplastin time, and thrombin time in rat blood. A bleeding time assay revealed that AfeE did not prolong bleeding time in mice at a dose of 1mg/kg. No acute cytotoxicity was observed for AfeE at 320μg/well in human umbilical vein endothelial cells. The afeE gene was cloned from the genome of Streptomyces sp. CC5. Full-length AFE-CC5E contained 434 amino acids and was processed into a mature form consisting 284 amino acids by posttranslational modification, as revealed by high-resolution mass spectrometry analysis. These results indicate that AfeE is a prospective candidate for antithrombotic drug development.

  9. Purification and characterization of a serine protease (CPM-2) with fibrinolytic activity from the dung beetles.

    Science.gov (United States)

    Ahn, Mi Young; Hahn, Bum-Soo; Ryu, Kang Sun; Hwang, Jae Sam; Kim, Yeong Shik

    2005-07-01

    Catharsius protease-2 (CPM-2) was isolated from the body of dung beetles, Catharsius molossus, using a three step purification process (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and affinity chromatography on DEAE Affi-Gel blue). The purified CPM-2, having a molecular weight of 24 kDa, was assessed homogeneously by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of CPM-2 was composed of X Val Gln Asp Phe Val Glu Glu Ile Leu. CPM-2 was inactivated by Cu2+ and Zn2+ and strongly inhibited by typical serine proteinase inhibitors such as TLCK, soybean trypsin inhibitor, aprotinin, benzamidine, and alpha1-antitrypsin. However, EDTA, EGTA, cysteine, beta-mercaptoethanol, E64, and elastatinal had little effect on enzyme activity. In addition, antiplasmin and antithrombin III were not sensitive to CPM-2. Based on the results of a fibrinolytic activity test, CPM-2 readily cleaved Aalpha- and Bbeta-chains of fibrinogen and fibrin, and gamma-chain of fibrinogen more slowly. The nonspecific action of the enzyme resulted in extensive hydrolysis, releasing a variety of fibrinopeptides of fibrinogen and fibrin. Polyclonal antibodies of CPM-2 were reactive to the native form of antigen. The ELISA was applied to detect quantities, in nanograms, of the antigen in CPM-2 protein.

  10. Ammodytase, a metalloprotease from Vipera ammodytes ammodytes venom, possesses strong fibrinolytic activity.

    Science.gov (United States)

    Leonardi, Adrijana; Fox, Jay W; Trampus-Bakija, Alenka; Krizaj, Igor

    2007-05-01

    Ammodytase, a high molecular mass metalloproteinase with fibrinogenolytic and fibrinolytic activities, was purified from long-nosed viper (Vipera ammodytes ammodytes) venom by gel filtration, affinity and ion-exchange chromatographies. The enzyme is a single-chain glycoprotein with apparent molecular mass of 70 kDa and isoelectric point of 6.6. Ammodytase shows very weak hemorrhagic activity, and only at doses higher than 20 microg. Consistent with this, it partially degrades some components of the extracellular matrix in vitro. It cleaves the Aalpha-chain of fibrinogen preferentially at peptide bonds Glu(441)-Leu(442) and Glu(539)-Phe(540). Its preference for bulky and hydrophobic amino acids at the P1' position in substrates is demonstrated by its hydrolysis of only the Gln(4)-His(5) and Tyr(16)-Leu(17) bonds in the B-chain of insulin. Ammodytase is able to dissolve fibrin clots. It neither activates nor degrades plasminogen and prothrombin, and has no effect on collagen- or ADP-induced platelet aggregation in vitro. LC/MS and MS/MS analyses of its tryptic fragments demonstrated that ammodytase is a P-III class snake venom metalloproteinase composed of metalloproteinase, disintegrin-like and cysteine-rich domains. Its similarity to hemorrhagins from V. a. ammodytes venom, accompanied by very low toxicity, makes ammodytase a promising candidate as an antigen to prepare antisera against these most dangerous components of the viper's venom. Moreover, its ability to degrade fibrin clots suggests its clinical use as an antithrombotic agent.

  11. Influence of decreased fibrinolytic activity and plasminogen activator inhibitor-1 4G/5G polymorphism on the risk of venous thrombosis.

    Science.gov (United States)

    Vuckovic, Biljana A; Djeric, Mirjana J; Tomic, Branko V; Djordjevic, Valentina J; Bajkin, Branislav V; Mitic, Gorana P

    2017-08-01

    : Objective of our study is to determine whether decreased fibrinolytic activity or plasminogen activator inhibitor (PAI)-1 4G/5G polymorphism influence the risk of venous thrombosis.Our case-control study included 100 patients with venous thrombosis, and 100 random controls. When patients were compared with random controls, unconditional logistic regression was used to calculate odds ratios (ORs) with 95% confidence intervals (CIs).Decreased fibrinolytic activity yielded a 2.7-fold increase in risk for venous thrombosis than physiological fibrinolytic activity (OR 2.70; 95% CI 1.22-5.98), when comparing patients with random controls. Adjustment for several putative confounders did not change the estimate (OR 3.02; 95% CI 1.26-7.22). Analysis of venous thrombotic risk influenced by PAI-1 genotype, showed no influence of PAI-1 4G/5G gene variant in comparison with 5G/5G genotype (OR 0.57 95% CI; 0.27-1.20).Decreased fibrinolytic activity increased, whereas PAI-1 4G/5G polymorphism did not influence venous thrombosis risk in this study.

  12. Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro

    Directory of Open Access Journals (Sweden)

    Ponnuswamy Vijayaraghavan

    2014-01-01

    Full Text Available A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice. Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme. Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach. Two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and sodium dihydrogen phosphate have significantly influenced enzyme production (P<0.05. A central composite design resulted in the production of 3699 U/mL of enzyme in the presence of 0.3% (w/w beef extract and 0.05% (w/w sodium dihydrogen phosphate, at 100% (v/w moisture after 72 h of fermentation. The enzyme production increased fourfold compared to the original medium. This enzyme was purified to homogeneity by ammonium sulfate precipitation, diethylaminoethyl-cellulose ion-exchange chromatography, Sephadex G-75 gel filtration chromatography, and casein-agarose affinity chromatography and had an apparent molecular mass of 29.5 kDa. The optimum pH and temperature for the activity of fibrinolytic enzyme were found to be 8.0 and 60°C, respectively. This enzyme was highly stable at wide pH range (7.0–9.0 and showed 27% ± 6% enzyme activity after initial denaturation at 60°C for 1 h. In vitro assays revealed that the enzyme could activate plasminogen and significantly degraded the fibrin net of blood clot, which suggests its potential as an effective thrombolytic agent.

  13. Effect of Atorvastatin intensive therapy on the serum inflammatory factors, platelet activity and fibrinolytic activity in patients with acute coronary syndrome

    Institute of Scientific and Technical Information of China (English)

    Xiao-Li Zhu; Yun Zhou; Fang Liu

    2016-01-01

    Objective:To observe the effect of Atorvastatin intensive therapy on the serum inflammatory factors, platelet activity and fibrinolytic activity in patients with acute coronary syndrome (ACS).Methods:A total of 92 patients with ACS were randomly divided into observation group (47 cases) and control group (45 cases). The control group was given Atorvastatin (10 mg/d) based on the conventional therapy, while the observation group was given Atorvastatin at an intensive dose (40 mg/d) based on the conventional therapy. Half a month later, the changes of IL-6, IL-8, hs-CRP, TNF-α, TXB2, GMP-140, PAI-1 and t-PA were observed and compared between the two groups.Results:After treatment, the inflammatory factors (IL-6, IL-8, hs-CRP and TNF-α) and the indicators of platelet activity (TXB2, GMP-140 and PAI-1) were obviously decreased, while the indicator of fibrinolytic activity (t-PA) was apparently increased in the two groups. Besides, the amplitudes of change referring to these indicators in the observation group were bigger than those in the control group after treatment, and the differences were statistically significant.Conclusion: The intensive therapy with the administration of Atorvastatin at a dose of 40 mg/d was better than the conventional therapy (Atorvastatin: 10 mg/d) in aspects of reducing inflammatory factors, inhibiting platelet activity and correcting the high coagulation state of fibrinolytic system.

  14. Thrombin inhibitory and clot-specific fibrinolytic activities of the urokinase variant, M23 (rscu-PA-40 kDa/Hir)

    NARCIS (Netherlands)

    Schneider, J; Wnendt, S; Saunders, D; HeinzelWieland, R; Wilffert, B; Steffens, G

    1996-01-01

    The recombinant bifunctional urokinase variant, M23 (rscu-PA-40 kDA/Hir), comprising the kringle and protease domain of single-chain urokinase-type plasminogen activator and a C-terminal fragment of hirudin in one single-chain molecule, was evaluated for its thrombin-inhibitory and fibrinolytic

  15. Characterization of Fibrinolytic Proteases from Gloydius blomhoffii siniticus Venom

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    Suk Ho Choi

    2011-09-01

    Full Text Available Objectives : This study was undertaken to identify fibrinolytic proteases from Gloydius blomhoffii siniticus venom and to characterize a major fibrinolytic protease purified from the venom. Methods: The venom was subjected to chromatography using columns of Q-Sepharose and Sephadex G-75. The molecular weights of fibrinolytic proteases showing fibrinolytic zone in fibrin plate assay were determined in SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis The effects of inhibitors and metal ions on fibrinolytic protease and the proteolysis patterns of fibrinogen, gelatin, and bovine serum albumin were investigated. Results : 1 The fibrinolytic fractions of the three peaks isolated from Gloydius blomhoffii siniticus venom contained two polypeptides of 46 and 59 kDa and three polypeptides of 32, 18, and 15 kDa and a major polypeptide of 54 kDa, respectively. 2 The fibrinolytic activity of the purified protease of 54 kDA was inhibited by metal chelators, such as EDTA, EGTA, and 1,10-phenanthroline, and disulfhydryl-reducing compounds, such as dithiothreitol and cysteine. 3 Calcium chloride promoted the fibrinolytic activity of the protease, but mercuric chloride and cobalt(II chloride inhibited it. 4 The fibrinolytic protease cleaved preferentially A-chain and slowly B-chain of fibrinogen. It also hydrolyzed gelatin but not bovine serum albumin. Conclusions: The Gloydius blomhoffii siniticus venom contained more than three fibrinolytic proteases. The major fibrinolytic protease was a metalloprotease which hydrolyzed both fibrinogen and gelatin, but not bovine serum albumin.

  16. EFFECT OF RADIX SALVIAE MILTIORRHIZAE ON THE PROCOAGULANT AND FIBRINOLYTIC ACTIVITIES INDUCED BY ENDOTOXIN-TREATED BOVINE ENDOTHELIAL CELLS

    Institute of Scientific and Technical Information of China (English)

    沈传陆; 张彩英; 王振义

    1992-01-01

    Effect of endotoxin, and endotoxin plus Radix Salviae Miltiorrhizae (RSM) on bovine aortic endothelial cells (BAECs) for the production of tissue factor (TF), plasminogen activator (PA) and plasminogen activator inhibitor (PAI) activities as well as prostacyclin (PGI2) content were studied. Stimulation of BAECs with endotoxin (1 μg/ ml) increased the expression of cell surface TF activity, the secretions of PAI activity and PGI2 content. However, PA activity in endotoxin-treated groups didn’t change significantly in comparison with that of the control. RSM could stimulate the secretion of PA activity and prevent the increase in TF and PAI activities from endotoxin-treated BAECs. However, there was no significant difference in PGI2 production between endotoxin-and endotoxin+RSM-treated BAECs. These results indicated that increased procoagulant function and decreased fibrinolytic activity of endothelial cells (ECs) might be one of the mechanisms of DIC in gram-negative septicemia and RSM would be a very important thug in the prevention and the treatment of DIC in gram-negative sepsis.

  17. A comparative analysis of the clotting and fibrinolytic activities of the snake venom (Bothrops atrox) from different geographical areas in Venezuela.

    Science.gov (United States)

    Salazar, Ana Maria; Rodriguez-Acosta, Alexis; Girón, Maria E; Aguilar, Irma; Guerrero, Belsy

    2007-01-01

    Venom constitution within the same snake species can present considerable geographical variations. Bothrops atrox venoms were obtained from adult snakes captured at different geographical locations: Parguasa (Bolívar state); Puerto Ayacucho 1, Serranía del Cuao and Puerto Ayacucho 2 (Amazon state). The coagulant and fibrinolytic activities of these venoms were compared. Amidolytic activity of crude snake venom was measured by a micromethod designed in our laboratory. Coagulant activity on plasma and fibrinogen due to thrombin-like activity in venoms was also determined. Crude snake venom fibrinolytic activity by the fibrin plate method was assayed. Chromatographic studies were developed on Protein-Pack 300 column. Polyacrylamide gel electrophoresis was carried out under reduced conditions. After SDS-PAGE of samples, the fibrin-zymography was tested on agarose-fibrin plates. The results demonstrated several differences among B. atrox venoms from different geographical areas. Chromatograms and SDS-PAGE profiles indicated that venoms from the same species presented differences in the molecular mass of their components. The procoagulant activity depended on the utilized method (amidolytic versus clotting). Parguasa and Puerto Ayacucho 2 venoms presented procoagulant activity for both methods. Furthermore, Parguasa venom had also the highest hemorrhagic activity and the lowest LD50. In relation to the fibrinolytic activity, Puerto Ayacucho 1 venom was the most active, equally for fibrin plates as for the amidolytic method (t-PA like). This venom had the lowest coagulant activity, which induced us to think that probably its procoagulant activity was interfered by its fibrinolytic activity.

  18. Statistical optimization of fibrinolytic enzyme production using agroresidues by Bacillus cereus IND1 and its thrombolytic activity in vitro.

    Science.gov (United States)

    Vijayaraghavan, Ponnuswamy; Vincent, Samuel Gnana Prakash

    2014-01-01

    A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice. Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme. Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach. Two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and sodium dihydrogen phosphate have significantly influenced enzyme production (P blood clot, which suggests its potential as an effective thrombolytic agent.

  19. Berberine-induced anticancer activities in FaDu head and neck squamous cell carcinoma cells.

    Science.gov (United States)

    Seo, Yo-Seob; Yim, Min-Ji; Kim, Bok-Hee; Kang, Kyung-Rok; Lee, Sook-Young; Oh, Ji-Su; You, Jae-Seek; Kim, Su-Gwan; Yu, Sang-Joun; Lee, Gyeong-Je; Kim, Do Kyung; Kim, Chun Sung; Kim, Jin-Soo; Kim, Jae-Sung

    2015-12-01

    In the present study, we investigated berberine‑induced apoptosis and the signaling pathways underlying its activity in FaDu head and neck squamous cell carcinoma cells. Berberine did not affect the viability of primary human normal oral keratinocytes. In contrast, the cytotoxicity of berberine was significantly increased in FaDu cells stimulated with berberine for 24 h. Furthermore, berberine increased nuclear condensation and apoptosis rates in FaDu cells than those in untreated control cells. Berberine also induced the upregulation of apoptotic ligands, such as FasL and TNF-related apoptosis-inducing ligand, and triggered the activation of caspase-8, -7 and -3, and poly(ADP ribose) polymerase, characteristic of death receptor-dependent extrinsic apoptosis. Moreover, berberine activated the mitochondria‑dependent apoptotic signaling pathway by upregulating pro-apoptotic factors, such as Bax, Bad, Apaf-1, and the active form of caspase-9, and downregulating anti-apoptotic factors, such as Bcl-2 and Bcl-xL. In addition, berberine increased the expression of the tumor suppressor p53 in FaDu cells. The pan-caspase inhibitor Z-VAD-fmk suppressed the activation of caspase-3 and prevented cytotoxicity in FaDu cells treated with berberine. Interestingly, berberine suppressed cell migration through downregulation of vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, and MMP-9. Moreover, the phosphorylation of extracellular signal-regulated kinase (ERK1/2) and p38, components of the mitogen-activated protein kinase pathway that are associated with the expression of MMP and VEGF, was suppressed in FaDu cells treated with berberine for 24 h. Therefore, these data suggested that berberine exerted anticancer effects in FaDu cells through induction of apoptosis and suppression of migration. Berberine may have potential applications as a chemotherapeutic agent for the management of head and neck squamous carcinoma.

  20. Study on Blood Coagulant/Fibrinolytic Activity at Plasma andMonocytic Levels in Coronary Heart Disease Patients withBlood-Stasis Syndrome of Traditional Chinese Medicine

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective: To explore and compare the changes of coagulant/fibrinolytic activity in coronary heart disease (CHD) patients with Blood Stasis Syndrome of TCM and evaluate the roles of these changes. Methods: Eighty patients of CHD were divided into two groups by Syndrome Differentiation of TCM, the Blood-Stasis (BS) group (30 cases) and the non-Blood-Stasis (NBS) group (50 cases, including 27 cases of Phlegm-Dampness Syndrome and 23 cases of Qi-Stagnation Syndrome); and 20 healthy persons were enrolled as normal control group. Tissue type plasminogen activator (t-PA) and its inhibitor (PAI-1) in plasma and in human peripheral blood monocyte cell (PBMC), as well as the procoagulant activity (PCA) in PBMC were measured by chromogenic substrate method. Results: The plasma PAI-1 activity and PCA of PBMC in the BS group were significantly higher than those in the NBS group and the normal control group (P<0.01). PAI-1 activity of PBMC in the two groups of CHD patients was higher than those in the normal control group significantly (P<0.01), but no significant difference was found between the BS group and the NBS group (P>0.05). The difference of plasma t-PA activity between the two groups of CHD was insignificant. The PBMC t-PA activity in the BS group was lower than that in the NBS and normal control groups (P<0.01). Conclusion: In the CHD patients with BS, the PBMC PCA was increased and the fibrinolytic activity at both plasma and monocyte levels lowered significantly, these changes in coagulant/fibrinolytic activity may be the important pathologic factors in forming BS which suggests that CHD patients with BS were in the prothrombotic state.

  1. Reduced PAK1 activity sensitizes FA/BRCA-proficient breast cancer cells to PARP inhibition.

    Science.gov (United States)

    Villamar Cruz, Olga; Prudnikova, Tatiana Y; Araiza-Olivera, Daniela; Perez-Plasencia, Carlos; Johnson, Neil; Bernhardy, Andrea J; Slifker, Michael; Renner, Catherine; Chernoff, Jonathan; Arias-Romero, Luis E

    2016-11-22

    Cells that are deficient in homologous recombination, such as those that have mutations in any of the Fanconi Anemia (FA)/BRCA genes, are hypersensitive to inhibition of poly(ADP-ribose) polymerase (PARP). However, FA/BRCA-deficient tumors represent a small fraction of breast cancers, which might restrict the therapeutic utility of PARP inhibitor monotherapy. The gene encoding the serine-threonine protein kinase p21-activated kinase 1 (PAK1) is amplified and/or overexpressed in several human cancer types including 25-30% of breast tumors. This enzyme controls many cellular processes by phosphorylating both cytoplasmic and nuclear substrates. Here, we show that depletion or pharmacological inhibition of PAK1 down-regulated the expression of genes involved in the FA/BRCA pathway and compromised the ability of cells to repair DNA by Homologous Recombination (HR), promoting apoptosis and reducing colony formation. Combined inhibition of PAK1 and PARP in PAK1 overexpressing breast cancer cells had a synergistic effect, enhancing apoptosis, suppressing colony formation, and delaying tumor growth in a xenograft setting. Because reduced PAK1 activity impaired FA/BRCA function, inhibition of this kinase in PAK1 amplified and/or overexpressing breast cancer cells represents a plausible strategy for expanding the utility of PARP inhibitors to FA/BRCA-proficient cancers.

  2. Ligand binding to the FA3-FA4 cleft inhibits the esterase-like activity of human serum albumin.

    Directory of Open Access Journals (Sweden)

    Paolo Ascenzi

    Full Text Available The hydrolysis of 4-nitrophenyl esters of hexanoate (NphOHe and decanoate (NphODe by human serum albumin (HSA at Tyr411, located at the FA3-FA4 site, has been investigated between pH 5.8 and 9.5, at 22.0°C. Values of Ks, k+2, and k+2/Ks obtained at [HSA] ≥ 5×[NphOXx] and [NphOXx] ≥ 5×[HSA] (Xx is NphOHe or NphODe match very well each other; moreover, the deacylation step turns out to be the rate limiting step in catalysis (i.e., k+3 a-shift appears to be correlated to the length of the fatty acid tail of the substrate. The inhibition of the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and 4-nitrophenyl myristate (NphOMy by five inhibitors (i.e., diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol has been investigated at pH 7.5 and 22.0°C, resulting competitive. The affinity of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol for HSA reflects the selectivity of the FA3-FA4 cleft. Under conditions where Tyr411 is not acylated, the molar fraction of diazepam, diflunisal, ibuprofen, and 3-indoxyl-sulfate bound to HSA is higher than 0.9 whereas the molar fraction of propofol bound to HSA is ca. 0.5.

  3. Gene expression of fibrinolytic factors urokinase plasminogen activator and plasminogen activator inhibitor-1 in rabbit temporo-mandibular joint cartilage with disc displacement

    Institute of Scientific and Technical Information of China (English)

    ZHAN Jing; GU Zhi-yuan; WU Li-qun; ZHANG Yin-kai; HU Ji-an

    2005-01-01

    Background The urokinase plasminogen activator system is believed to play an important role in degradation of the extracellular matrix associated with cartilage and bone destruction; however its precise roles in temporomandibular disorders have not yet been clarified. The aims of this study were to investigate the gene expression of fibrinolytic factors urokinase plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) in the articular cartilage of rabbit temporomandibular joint (TMJ) with disc displacement (DD) and to probe the relationship between fibrinolytic activity and cartilage remodeling. Methods Disc displacement of right joints was performed in 36 of 78 rabbits under investigation. The animals were sacrificed at 4 days and 1, 2, 4, 8 and 12 weeks after surgery, respectively. The right joints of these animals were harvested and processed for the examination of mRNA expression of uPA and PAI-1 in articular cartilage using in situ hybridization techniques. Results The expression of uPA and PAI-1 was co-expressed weakly in the chondrocytes from transitive zone to hypertrophic zone and mineralized zone, while no hybridizing signals were shown in proliferative zone and superficial zone in control rabbits. The most striking was the up-regulation of uPA and PAI-1 mRNA in 4-day rabbits postoperatively at the onset of cartilage degeneration. The strongest hybridizing signals for uPA and PAI-1 were seen in 2-week rabbits postoperatively. After 2 weeks, the expression of uPA and PAI-1 began to decrease and reached nearly normal level at 12 weeks. Conclusions The expression of the uPA/PAI-1 system coincides with the pathological changes in condylar cartilage after DD. The uPA/PAI-1 system may be one of the essential mediators in articular cartilage remodeling.

  4. [Inhibition of esterase by L-lysine, the activator and fibrinolytic activity of the plasmin-streptokinase activator complex].

    Science.gov (United States)

    Aĭsina, R B; Gaĭsarian, E S; Snitko, Ia E; Varfolomeev, S D

    1994-02-01

    The effect of L-lysine on some reactions catalysed by plasmin and the plasmin-streptokinase activator complex has been studied. The constants for competitive inhibition by L-lysine of the benzyloxycarbonyl-L-lysine p-nitrophenyl ester hydrolysis by the activator (Ki 116 mM), of the plasminogen activation by the activator (Ki 8 mM) and of fibrinolysis by plasmin (Ki 3 mM) were determined. It was found that L-lysine at concentrations below 0.05 M, which do not affect the activator's esterase activity, does inhibit fibrinolysis by plasmin and the activator complex. The effect of L-lysine on fibrinolysis under the action of the activator is complex: it inhibits both the activation of clot-entrapped plasminogen by the activator and lysis of fibrin by the plasmin formed. This inhibitory action of L-lysine is largely related to the fact that it lowers the sorption of the activator, plasminogen and plasmin on fibrin, competing with fibrin for their lysine-binding sites as well as worsens the activator-plasminogen binding.

  5. Semi-automatic method for routine evaluation of fibrinolytic components.

    Science.gov (United States)

    Collen, D; Tytgat, G; Verstraete, M

    1968-11-01

    A semi-automatic method for the routine evaluation of fibrinolytic activity is described. The principle is based upon graphic recording by a multichannel voltmeter of tension drops over a potentiometer, caused by variations in the influence of light upon a light-dependent resistance, resulting from modifications in the composition of the fibrin fibres by lysis. The method is applied to the assessment of certain fibrinolytic factors with widespread fibrinolytic endpoints, and the results are compared with simultaneously obtained visual data on the plasmin assay, the plasminogen assay, and on the euglobulin clot lysis time.

  6. 广西莪术及其茎叶多糖的体外纤溶活性研究%Vitro fibrinolytic activities of polysaccharides from Rhizome and stem and its leaf of Curcuma kwangsiensis

    Institute of Scientific and Technical Information of China (English)

    曾金强; 潘小姣; 陈秋燕; 唐璐

    2012-01-01

    目的 考察广西莪术及其茎叶多糖的体外纤溶活性.方法 用分步沉淀法,分离广西莪术及其茎叶的多糖,然后用纤维蛋白琼脂平板法考察不同醇沉多糖的纤溶活性.结果 广西莪术只有10%醇浓度沉淀多糖的体外纤溶效果稳定.广西莪术茎叶10%~30%的醇浓度沉淀多糖体外纤溶作用稳定,40%~60%的醇浓度沉淀多糖在高、中浓度时作用稳定,在低浓度时作用不稳定;在有纤溶活性的分步沉淀多糖中,除了10%和30%的醇浓度沉淀多糖具有浓度依赖性之外(P < 0.05),其余纤溶活性多糖均无浓度依赖性.结论 广西莪术及其茎叶的多糖成分具有体外纤溶活性.%Objective To observe the vitro fibrinolytic activities of polysaccharides from Rhizome and its stem and leaf of Curcuma kwangsiensis. Methods Fractional precipitation was used to separate the different polysaccharides and fibrin plate method was used to detect their vitro fibrinolytic activities. Results Only the 10% alcohol precipitated polysaccharides from Rhizome of Curcuma kwangsiensis had stable vitro fibrinolytic activities. The polysaccharides having.stable vitro fibrinolytic activities from stem and leaf of Curcuma kwangsiensis were the 10% to 30% alcohol precipitated polysaccharides and the high and medium concetration of 40% to 60% alcohol precipitated polysaccharides. 10% and 30% alcohol precipitated polysaccharides had vitro fibrinolytic activities in concentration dependent manner (P < 0.05). Other fibrinolytic activities polysaccharides had no concentra-tion-dependent. Conclusion Polysaccharides from rhizome, stem and leaf of Curcuma kwangsiensis has vitro fibrinolytic activities.

  7. 具有溶栓活性的重组乳酸乳球菌的构建%Construction of recombinant Lactococcus lactis with fibrinolytic activity

    Institute of Scientific and Technical Information of China (English)

    余凤云; 冯浩; 许芳; 闫达中

    2011-01-01

    Pro-nattokinase gene was amplified by PCR from Bacillus subtilis subsp.natto genomic DNA, which was cloned into pGT-19 vector and then sequenced.The recombinant plasmid pFY002 was constructed by inserting pro-nattokinase gene into expression vector pNZ8048 and was transformed into Lactococcus lactis NZ9000.The fibrinolytic activity was analyzed after the expression of gene was induced by nisin A.The optimum conditions including nisinA concentration, temperature and induction time in the engineering L.lactis were further investigated.%利用PCR方法从纳豆芽孢杆菌(Bacillus subtilis subsp.natto)基因组DNA中扩增纳豆激酶原基因,构建了纳豆激酶原基因的表达载体pFY002,转化LactococcuslactisNZ9000,得到活性表达纳豆激酶的重组菌LactococcuslactisNZ9000/pFY002.利用纤维蛋白平板法检测其溶栓活性,并对诱导剂乳链菌肽(NisinA)的浓度、温度、诱导时间对重组菌产酶的影响进行探究.

  8. Mesoglycan treatment restores defective fibrinolytic potential in cutaneous necrotizing venulitis.

    Science.gov (United States)

    Lotti, T; Celasco, G; Tsampau, D; Teofoli, P; Benci, M; Dahm, M; Comacchi, C; Ghersetich, I; Matucci-Cerinic, M

    1993-05-01

    Cutaneous necrotizing venulitis (CNV) is a clinical disorder associated with segmental inflammation and fibrinoid necrosis of the dermal venules. It usually presents clinically as palpable purpura, even sometimes as nodules, bullae, ulcers, and urticarial lesions. This form, when showing as leukocytoclastic vasculitis is apparently characterized by the tissue deposition of circulating immune complexes and by reduced cutaneous (CFA) and plasma (PFA) fibrinolytic activity due to reduced release of plasminogen activator (PA) from the venular endotheliocytes. Reduced CFA and PFA cause large amounts of fibrin deposits in both intra- and perivascular areas, which are able to magnify and self perpetuate the inflammatory processes following immune complex deposition. We have studied both the PFA and CFA potential (the maximum amount of PA released in the skin after certain stimuli) and the deposits of immunoglobulins, C3, and fibrin related antigen, before and after intradermal injection of histamine (a substance able to provoke endothelial release of PA), in three subjects affected by CNV before and 20 days after 10 mg/kg/day I.M. treatment with the fibrinolytic agent mesoglycan. Cutaneous fibrinolytic activity and CFA potential, reduced prior to treatment, was normal after treatment, while the deposits of immunoglobulins (IgA, IgG and IgM), C3, and fibrin related antigen, detected with direct immune fluorescence (DIF) showed similar findings before and after treatment. These data suggest that reduced CFA may play a major role in the pathogenesis of the immunologically mediated injury in CNV. The intraperivascular deposition of fibrin is favored. The fibrinolytic agent mesoglycan seems effective in restoring defective fibrinolysis in patients affected by cutaneous necrotizing venulitis, suggesting that in cases with reduced cutaneous fibrinolytic activity (or potential) the use of a fibrinolytic agent should be considered.

  9. Fibrinolytic and factor XIII activity in subarachnoid hemorrhage Actividad fíbrinolítica y del factor XIII en la hemorragia subaracnoidea

    Directory of Open Access Journals (Sweden)

    Luis A. Pliego

    1997-09-01

    Full Text Available The balance between fibrinolytic activity and coagulation mechanisms seems to play an important role in the rebleeding of a subarachnoid hemorrhage (SAH due to aneurysmatic rupture. In the present paper we describe our findings in a group of patients (n10 with S AH. The plasmatic levels of fibrinogen and their degradation products (FDP, APTT, prothrombin activity and factor XIII were determined within 72 hours of initial bleeding, or of eventual rebleeding. Factor XIII activity in the first bleeding was 82.1 ±4%, while the levels of FDP were 3.8±lmg/ml. In patients presenting rebleeding (n4, Factor XIII activity was 67.3±4.5% the day it manifested, which is significantly less than the values previously observed (pEl balance entre la actividad fíbrinolítica y los mecanismos de la coagulación juegan un rol importante en el resangrado de una hemorragia subaracnoidea debido a ruptura aneurismástica. En el presente estúdio se describen nuestros hallazgos en un grupo de pacientes (n10 con hemorragia subaracnoidea (SAH. Los niveles plasmaticos de fibrinogeno y sus productos de degradation (FDP, la actividad de protrombina (APTT y de factor XIII fueron determinados dentro de las 72 hs, dei sangrado inicial y ante el eventual resangrado. La actividad de factor XIII en el primer sangrado fue de 82,1±4%, mientras el nivel de FDP fue de 3,8±lmg/ml. En aquellos pacientes que resangraron (n4, la actividad de factor XIII fue de 67,3+4,5%, el dia del resangrado, el cual es significativamente menor que los valores previamente observados (p<0.01, mientras que los niveles de FDP fueron 4,1± 2mg/ml. La reduction de la actividad de factor XIII sugiere un importante rol de este en la establilidad del coágulo en el sitio de ruptura aneurismática, siendo posible atribuir un valor predicitivo a la reduction de su actividad.

  10. Haemarthrosis stimulates the synovial fibrinolytic system in haemophilic mice

    NARCIS (Netherlands)

    Nieuwenhuizen, Laurens; Roosendaal, Goris; Coeleveld, Katja; Lubberts, Erik; Biesma, Douwe H.; Lafeber, Floris P. J. G.; Schutgens, Roger E. G.

    2013-01-01

    Recurrent joint bleeding is the most common manifestation of haemophilia resulting in haemophilic arthropathy (HA). The exact pathophysiology is unknown, but it is suggested that arthropathy is stimulated by liberation of fibrinolytic activators from the synovium during haemarthrosis. The aim of thi

  11. Global fibrinolytic activity, PAI-1 level, and 4G/5G polymorphism in Thai children with arterial ischemic stroke.

    Science.gov (United States)

    Natesirinilkul, Rungrote; Sasanakul, Werasak; Chuansumrit, Ampaiwan; Kadegasem, Praguywan; Visudtibhan, Anannit; Wongwerawattanakoon, Pakawan; Sirachainan, Nongnuch

    2014-01-01

    Prolonged euglobulin clot lysis time (ECLT) and increased level of plasminogen activator inhibitor-1 (PAI-1) were reported to be risk factors of arterial ischemic stroke (AIS) by some studies; however, these findings were not supported by other studies. The objective of this study was to determine the association of ECLT, PAI-1 level, and polymorphisms of 4G and 5G of PAI-1 gene to the development of AIS in Thai children. This study included patients aged 1-18 years old. Diagnosis of AIS was confirmed by imaging study. The control group was age- and sex-matched healthy subjects. Demographic data were recorded, and blood was tested for ECLT, PAI-1 level, lipid profiles, fasting blood sugar (FBS), and 4G and 5G polymorphisms of PAI-1 gene. There were 70 subjects participating in this study, consisting of 30 patients and 40 controls. Demographic data, lipid profiles, and FBS were similar between the 2 groups. Furthermore, ECLT and PAI-1 level did not differ between patient and control groups; however, both showed significant correlation (r = .352, P = .006). The 4G/5G polymorphism was the most common genotype in both patient and control groups (69.0% vs. 80.0%). However, 4G and 5G polymorphisms of PAI-1 gene did not correlate with PAI-1 level in this study (P = .797). The PAI-1 level and 4G/5G polymorphism may not be a risk factor of AIS in this population. It was also found that the 4G/5G polymorphism was the most common PAI-1 genotype in this study. Copyright © 2014 National Stroke Association. Published by Elsevier Inc. All rights reserved.

  12. Fibrinolytic enzyme from newly isolated marine bacterium Bacillus subtilis ICTF-1: media optimization, purification and characterization.

    Science.gov (United States)

    Mahajan, Prafulla M; Nayak, Shubhada; Lele, Smita S

    2012-03-01

    Fibrinolytic enzymes are important in treatment of cardiovascular diseases. The present work reports isolation, screening and identification of marine cultures for production of fibrinolytic enzymes. A potent fibrinolytic enzyme-producing bacterium was isolated from marine niches and identified as Bacillus subtilis ICTF-1 on the basis of the 16S rRNA gene sequencing and biochemical properties. Further, media optimization using L(18)-orthogonal array method resulted in enhanced production of fibrinolytic enzyme (8814 U/mL) which was 2.6 fold higher than in unoptimized medium (3420 U/mL). In vitro assays revealed that the enzyme could catalyze blood clot lysis effectively, indicating that this enzyme could be a useful thrombolytic agent. A fibrinolytic enzyme was purified from the culture supernatant to homogeneity by three step procedures with a 34.42-fold increase in specific activity and 7.5% recovery. This purified fibrinolytic enzyme had molecular mass of 28 kDa, optimal temperature and pH at 50 °C and 9, respectively. It was stable at pH 5.0-11.0 and temperature of 25-37 °C. The enzyme activity was activated by Ca(2+) and obviously inhibited by Zn(2+), Fe(3)(+), Hg(2+) and PMSF. The purified fibrinolytic enzyme showed high stability towards various surfactants and was relatively stable towards oxidizing agent. Considering these properties purified fibrinolytic enzyme also finds potential application in laundry detergents in addition to thrombolytic agent. The gene encoding fibrinolytic enzyme was isolated and its DNA sequence was determined. Compared the full DNA sequence with those in NCBI, it was considered to be a subtilisin like serine-protease.

  13. Fibrinolytic agents in the treatment of intraventricular hemorrhage in adults

    Science.gov (United States)

    Haines; Lapointe

    1998-05-13

    This paper aims to review current literature on the treatment of acute intraventricular hemorrhage in adults with intraventricular infusion of fibrinolytic agents. A literature search on the topics of "intraventricular hemorrhage" or "intracerebral hemorrhage" with "thrombolytic therapy", "fibrinolytic therapy", "urokinase", "streptokinase", "tissue plasminogen activator" or "tPA" covering the years 1966-1997 was carried out electronically. This was supplemented by searching the reference lists of the identified articles. Articles regarding exclusively intracerebral hemorrhage or hematoma, neonatal intraventricular hemorrhage, non-therapeutic issues, and laboratory research were excluded. The included articles are summarized in evidence and evaluation tables. Six articles evaluating the treatment of intraventricular hemorrhage in adults with intraventricular fibrinolytic agents were identified. One reports a small randomized clinical trial including 16 patients and appears to show a statistically insignificant preference for urokinase treatment. Five other reports present case series for which a total of 58 patients were exposed to either streptokinase, urokinase, or recombinant tissue plasminogen activator (rt-PA) and suggest good outcome. Two of them were with non-randomized retrospective or prospective controls, and three have no controls. Despite important limitations, all reports suggest that blood is more rapidly cleared from the ventricles and outcome is better when administering a fibrinolytic agent intraventricularly. While the experience presented in these papers suggests that intraventricular administration of fibrinolytic agents may be associated with fewer complications, more rapid clearing of blood from the ventricles, less late hydrocephalus, and better long-term outcome than is seen in patients treated with ventricular drainage alone, it is insufficient to recommend such treatment as a matter of policy. Substantial methodologic flaws render these

  14. Cloning and Expression of One Fibrinolytic Enzyme from Bacillus sp.zlw-2

    Institute of Scientific and Technical Information of China (English)

    NAN Xin-mian; GUO Run-fang; YU Hong-wei; JIA Ying-min

    2009-01-01

    The gene encoding fibrinolytic enzyme from Bacillus sp.zlw-2 was cloned and sequenced (accession no.EU734749),which was 1146 bp,encoded 381 amino acids and had 99% homology with Nattokinase YF308 and NAT.The genes encoding pre-pro-fibrinolytic enzyme (including signal peptide,propeptide,and mature peptide) and fibrinolytic enzyme (including mature peptide) were cloned into pET28a vector respectively and then transformed into Escherichia coil BL21 (DE3).The recombinant of pre-pro-fibrinolytic enzyme showed enzyme activity of 183 U mL-1,while no detectable enzyme activity could be found from the recombinant of the mature peptide.

  15. Carbohydrate derived fulvic acid (CHD-FA: an in vitro investigation of a novel membrane active antiseptic agent against Candida albicans biofilms

    Directory of Open Access Journals (Sweden)

    Leighann eSherry

    2012-03-01

    Full Text Available Carbohydrate derived fulvic acid (CHD-FA is a heat stable low molecular weight, water soluble, cationic, colloidal material with proposed therapeutic properties. The aim of this study was to evaluate the antifungal activity of CHD-FA against Candida albicans, and to characterise its mode of action. A panel of C. albicans isolates (n=50 derived from a range of clinical specimens were grown planktonically and as biofilms, and the minimum inhibitory concentrations (MICs determined. Scanning electron microscopy was performed to examine ultrastructural changes and different cell membrane assays were used to determine its mode of action. In addition, the role of C. albicans biofilm resistance mechanisms were investigated to determine their effects of CHD-FA activity. CHD-FA was active against planktonic and sessile C. albicans at concentrations 0.125% and 0.25% respectively, and was shown to be fungicidal, acting through disruption of the cell membrane activity. Resistance mechanisms, including matrix, efflux and stress, had a limited role upon CHD-FA activity. Overall, based on the promising in vitro spectrum of activity and minimal biofilm resistance of the natural and cheap antiseptic CHD-FA, further studies are required to determine its applicability for clinical use.

  16. Glucomannan and glucomannan plus spirulina added to pork significantly block dietary cholesterol effects on lipoproteinemia, arylesterase activity, and CYP7A1 expression in Zucker fa/fa rats.

    Science.gov (United States)

    González-Torres, Laura; Vázquez-Velasco, Miguel; Olivero-David, Raúl; Bastida, Sara; Benedí, Juana; González, Rafaela Raposo; González-Muñoz, Ma José; Sánchez-Muniz, Francisco J

    2015-12-01

    Zucker fa/fa rats easily develop dyslipidemia and obesity. Restructured pork (RP) is a suitable matrix for including functional ingredients. The effects of glucomannan- RP or glucomannan plus spirulina-enriched RP on plasma lipid/lipoprotein levels, cytochrome P450 7A1 (CYP7A1) expression, and arylesterase activity in growing fa/fa rats fed high-energy, high-fat cholesterol-enriched diets were tested. Groups of six rats each received diet containing 15% control-RP (C), 15% glucomannan-RP diet (G), 15% glucomannan + spirulina-RP diet (GS), and same diets enriched with 2.4% cholesterol and 0.49% cholic acid (cholesterol-enriched control (HC), cholesterol-enriched glucomannan (HG), and cholesterol-enriched glucomannan + spirulina (HGS) diets) over a 7-week period. C diet induced obesity, severe hyperglycemia, moderate hypercholesterolemia, and hypertriglyceridemia. Those facts were not significantly modified by G or GS diets. G diet increased CYP7A1 expression but decreased the total cholesterol/high density lipoproteins (HDL)-cholesterol ratio (p CYP7A1 expression. HC vs. C diet reduced food intake, body weight gain, and plasma glucose (p CYP7A1 expression (at least p CYP7A1 expression (p < 0.01) but did not modify the cholesterol/HDL-cholesterol ratio. HGS vs. HG decreased triglyceridemia, the triglyceride-glucose (TyG) index and increased arylesterase/HDL-cholesterol activity (p < 0.05). In conclusion, G- and GS-RP act as functional foods and notably blocked the dietary cholesterol effects. In addition, HGS-RP improved the glucomannan hypolipidemic effects, increased arylesterase/HDL-cholesterol activity, and decreased insulin resistance.

  17. Production, optimization and characterization of fibrinolytic enzyme by Bacillus subtilis RJAS19.

    Science.gov (United States)

    Kumar, D J Mukesh; Rakshitha, R; Vidhya, M Annu; Jennifer, P Sharon; Prasad, Sandip; Kumar, M Ravi; Kalaichelvan, P T

    2014-04-01

    The present study aimed at the production, purification and characterization of fibrinolytic nattokinase enzyme from the bacteria isolated from natto food. For the purpose, a fibrinolytic bacterium was isolated and identified as Bacillus subtilis based on 16S rDNA sequence analysis. The strain was employed for the production and optimization of fibrinolytic enzyme. The strain showed better enzyme production during 72nd h of incubation time with 50 degrees C at the pH 9. The lactose and peptone were found to be increasing the enzyme production rate. The enzyme produced was purified and also characterized with the help of SDS-PAGE analysis. The activity and stability profile of the purified enzyme was tested against different temperature and pH. The observations suggesting that the potential of fibrinolytic enzyme produced by Bacillus subtilis RJAS 19 for its applications in preventive medicines.

  18. Multispectral Radiometric Analysis of Façades to Detect Pathologies from Active and Passive Remote Sensing

    Directory of Open Access Journals (Sweden)

    Susana Del Pozo

    2016-01-01

    Full Text Available This paper presents a radiometric study to recognize pathologies in façades of historical buildings by using two different remote sensing technologies covering part of the visible and very near infrared spectrum (530–905 nm. Building materials deteriorate over the years due to different extrinsic and intrinsic agents, so assessing these affections in a non-invasive way is crucial to help preserve them since in many cases they are valuable and some have been declared monuments of cultural interest. For the investigation, passive and active remote acquisition systems were applied operating at different wavelengths. A 6-band Mini-MCA multispectral camera (530–801 nm and a FARO Focus3D terrestrial laser scanner (905 nm were used with the dual purpose of detecting different materials and damages on building façades as well as determining which acquisition system and spectral range is more suitable for this kind of studies. The laser scan points were used as base to create orthoimages, the input of the two different classification processes performed. The set of all orthoimages from both sensors was classified under supervision. Furthermore, orthoimages from each individual sensor were automatically classified to compare results from each sensor with the reference supervised classification. Higher overall accuracy with the FARO Focus3D, 74.39%, was obtained with respect to the Mini MCA6, 66.04%. Finally, after applying the radiometric calibration, a minimum improvement of 24% in the image classification results was obtained in terms of overall accuracy.

  19. An Active Smart Material Control System for F/A-18 Buffet Alleviation

    Science.gov (United States)

    Sheta, Essam F.; Moses, Robert W.; Huttsell, Lawrence J.; Harrand, Vincent J.

    2003-01-01

    The vertical tail buffet problem of fighter aircraft occurs at high angles of attack when the vortical flow breaks down ahead of the vertical tails resulting in unsteady and unbalanced pressure loads on the vertical tails. The buffet loads imposed upon the vertical tails resulted in a premature fatigue failure of the tails, and consequently limits the performance and super maneuverability of twin-tail fighter aircraft. An active smart material control system using distributed piezoelectric actuators has been developed for buffet alleviation and is presented. The inboard and outboard surfaces of the vertical tail are equipped with piezoelectric actuators to control the buffet responses in the first bending and torsion modes. The electrodynamics of the piezoelectric actuators are expressed with a three-dimensional finite-element model. A single-input-single-output controller is designed to drive the active piezoelectric actuators. High-fidelity multidisciplinary analysis modules for the fluid dynamics, structure dynamics, electrodynamics of the piezoelectric actuators, control law, fluid structure interfacing, and grid motion are integrated into a multidisciplinary computing environment that controls the temporal synchronization of the analysis modules. At 30 degree angle of attack, RMS values of tip acceleration are reduced by as much as 12%. The peak values of the power spectral density of tail-tip acceleration are reduced by as much as 22% in the first bending mode and by as much as 82% in the first torsion mode. The actively controlled piezoelectric actuators were also effective in adding damping at wide range of angles of attack.

  20. Increased tissue-type plasminogen activator antigen release is not accompanied by increased systemic fibrinolytic activity in severe neonatal respiratory distress syndrome

    NARCIS (Netherlands)

    Brus, F; Oetomo, SB; Schieving, J; Groothuis, E; Okken, A; van Oeveren, W

    1999-01-01

    Intravascular and intraalveolar fibrin depositions in preterm infants with severe respiratory distress syndrome (RDS) have been attributed to activation of clotting. We questioned whether in the face of activated clotting, Fibrinolysis is sufficient in these infants. We found, in infants with severe

  1. Functional characterization of fibrinolytic metalloproteinases (colombienases) isolated from Bothrops colombiensis venom.

    Science.gov (United States)

    Girón, María E; Guerrero, Belsy; Salazar, Ana María; Sánchez, Elda E; Alvarez, Marco; Rodríguez-Acosta, Alexis

    2013-11-01

    Researchers trying to improve the safety and efficacy of fibrinolytic therapy have been isolating fibrinolytic enzymes from snake venoms. Two fibrinolytic enzymes, colombienases 1 and 2, with significant activity have been isolated from the venom of Bothrops colombiensis. The colombienases were characterized for various biological activities which include hemorrhagic, fibrinogenolytic, proteolytic, hemolytic, edematogenic and cytotoxic. Colombienases directly acted on fibrin by degrading fibrinogen Aα and Bβ chains without activating the fibrinolytic system (plasminogen/plasmin), additionally colombienase-2 degraded fibrinogen γ chains as well as the fibronectin molecule. Laminin and type IV collagen were colombienases resistant. Gelatin-zymogram activity was present in B. colombiensis venom (BcV) bands between 64 and 148 kDa. All activities were abolished by metalloproteinases inhibitors. Both enzymes lacked hemorrhagic, hemolytic, cytotoxic, plasminogen activator and coagulant activities. Both colombienases had direct fibrino(geno)lytic activity without other toxic side effects including in vivo hemorrhaging, which could be promising in terms of therapeutic potential as thrombolytic agents. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Cow dung is a novel feedstock for fibrinolytic enzyme production from newly isolated Bacillus sp. IND7 and its application in in vitro clot lysis

    Directory of Open Access Journals (Sweden)

    Ponnuswamy eVijayaraghavan

    2016-03-01

    Full Text Available Bacterial fibrinolytic enzymes find great applications to treat and prevent cardiovascular diseases. The novel fibrinolytic enzymes from food grade organisms are useful for thrombolytic therapy. This study reports fibrinolytic enzyme production by Bacillus sp. IND7 in solid-state fermentation (SSF. In this study, cow dung was used as the cheap substrate for the production of fibrinolytic enzyme. Enzyme production was primarily improved by optimizing the nutrient and physical factors by one-variable-at-a-time approach. A statistical method (two-level full factorial design was applied to investigate the significant variables. Of the different variables, pH, starch, and beef extract significantly influenced on the production of fibrinolytic enzyme (p < 0.05. The optimum levels of these significant factors were further investigated using response surface methodology. The optimum conditions for enhanced fibrinolytic enzyme production were 1.23% (w/w starch and 0.3 % (w/w beef extract with initial medium pH 9.0. Under the optimized conditions, cow dung substrate yielded 8,345 U/g substrate, and an overall 2.5-fold improvement in fibrinolytic enzyme production was achieved due to its optimization. This is the first report of fibrinolytic enzyme production using cow dung substrate from Bacillus sp. in SSF. The crude enzyme displayed potent activity on zymography and digested goat blood clot completely in in vitro condition.

  3. Urokinase plasminogen receptor and the fibrinolytic complex play a role in nerve repair after nerve crush in mice, and in human neuropathies.

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    Cristina Rivellini

    Full Text Available Remodeling of extracellular matrix (ECM is a critical step in peripheral nerve regeneration. In fact, in human neuropathies, endoneurial ECM enriched in fibrin and vitronectin associates with poor regeneration and worse clinical prognosis. Accordingly in animal models, modification of the fibrinolytic complex activity has profound effects on nerve regeneration: high fibrinolytic activity and low levels of fibrin correlate with better nerve regeneration. The urokinase plasminogen receptor (uPAR is a major component of the fibrinolytic complex, and binding to urokinase plasminogen activator (uPA promotes fibrinolysis and cell movement. uPAR is expressed in peripheral nerves, however, little is known on its potential function on nerve development and regeneration. Thus, we investigated uPAR null mice and observed that uPAR is dispensable for nerve development, whereas, loss of uPAR affects nerve regeneration. uPAR null mice showed reduced nerve repair after sciatic nerve crush. This was a consequence of reduced fibrinolytic activity and increased deposition of endoneurial fibrin and vitronectin. Exogenous fibrinolysis in uPAR null mice rescued nerve repair after sciatic nerve crush. Finally, we measured the fibrinolytic activity in sural nerve biopsies from patients with peripheral neuropathies. We showed that neuropathies with defective regeneration had reduced fibrinolytic activity. On the contrary, neuropathies with signs of active regeneration displayed higher fibrinolytic activity. Overall, our results suggest that enforced fibrinolysis may facilitate regeneration and outcome of peripheral neuropathies.

  4. Urokinase Plasminogen Receptor and the Fibrinolytic Complex Play a Role in Nerve Repair after Nerve Crush in Mice, and in Human Neuropathies

    Science.gov (United States)

    Rivellini, Cristina; Dina, Giorgia; Porrello, Emanuela; Cerri, Federica; Scarlato, Marina; Domi, Teuta; Ungaro, Daniela; Carro, Ubaldo Del; Bolino, Alessandra; Quattrini, Angelo; Comi, Giancarlo; Previtali, Stefano C.

    2012-01-01

    Remodeling of extracellular matrix (ECM) is a critical step in peripheral nerve regeneration. In fact, in human neuropathies, endoneurial ECM enriched in fibrin and vitronectin associates with poor regeneration and worse clinical prognosis. Accordingly in animal models, modification of the fibrinolytic complex activity has profound effects on nerve regeneration: high fibrinolytic activity and low levels of fibrin correlate with better nerve regeneration. The urokinase plasminogen receptor (uPAR) is a major component of the fibrinolytic complex, and binding to urokinase plasminogen activator (uPA) promotes fibrinolysis and cell movement. uPAR is expressed in peripheral nerves, however, little is known on its potential function on nerve development and regeneration. Thus, we investigated uPAR null mice and observed that uPAR is dispensable for nerve development, whereas, loss of uPAR affects nerve regeneration. uPAR null mice showed reduced nerve repair after sciatic nerve crush. This was a consequence of reduced fibrinolytic activity and increased deposition of endoneurial fibrin and vitronectin. Exogenous fibrinolysis in uPAR null mice rescued nerve repair after sciatic nerve crush. Finally, we measured the fibrinolytic activity in sural nerve biopsies from patients with peripheral neuropathies. We showed that neuropathies with defective regeneration had reduced fibrinolytic activity. On the contrary, neuropathies with signs of active regeneration displayed higher fibrinolytic activity. Overall, our results suggest that enforced fibrinolysis may facilitate regeneration and outcome of peripheral neuropathies. PMID:22363796

  5. Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract.

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    Willy Praira

    2010-11-01

    Full Text Available Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract. Ediblestraw mushroom (V. volvaceae has been known used for improvement of blood circulation due to its fibrinolyticcontent. The objective of the study is to purify and characterize fibrinolytic protease from straw mushroom extract.Purification were performed through several steps, i.e. precipitation using ammonium sulphate 75%, dialyzed membran(cut-off 10 kDa, and ion-exchange chromatography using DEAE Sepharose. The active fraction of DEAE-Sepharosecontains two purified protein bands with molecular weight of 12.9 and 15.8 kDa. The active fraction has specificactivity of 0.383 U/mg with 2.7 fold higher purification compared to its crude extract. Both crude and purified enzymeshad optimum activity at temperature of 50 ºC and pH 7 in 10 minutes of incubation. Fibrin zymographic profiledemonstrated that the enzyme hydrolyzed fibrin, as well as casein, indicating their potent fibrinolytic activity. Theenzyme was strongly inhibited by phenilmethylsulphonyl fluoride and N-p-tosil-L-lysinchloromethyl keton. Thissuggested that it was a serine protease. In summary, these results showed that crude and purified protease of strawmushroom (V. volvaceae has fibrinolytic activities that can be applied for alternative thrombolytic therapy.

  6. Angiogenic and fibrinolytic factors in blood during the first half of pregnancy and adverse pregnancy outcomes.

    NARCIS (Netherlands)

    Coolman, M.; Timmermans, S.; Groot, C.J. de; Russcher, H.; Lindemans, J.; Hofman, A.; Geurts-Moespot, A.; Sweep, F.C.; Jaddoe, V.V.; Steegers, E.A.P.

    2012-01-01

    OBJECTIVE: To estimate whether the imbalance of angiogenic factors (soluble fms-like tyrosine kinase-1, placental growth factor) and fibrinolytic factors (plasminogen activator inhibitor-2 [PAI-2]) might affect placentation in early pregnancy. METHODS: We studied the associations of maternal soluble

  7. A newly derived protein from Bacillus subtilis natto with both antithrombotic and fibrinolytic effects.

    Science.gov (United States)

    Omura, Kazunobu; Hitosugi, Masahito; Zhu, Xia; Ikeda, Masashi; Maeda, Hiroaki; Tokudome, Shogo

    2005-11-01

    Natto, steamed soybeans fermented by Bacillus subtilis natto, is a traditional Japanese food. We derived a purified protein layer, called NKCP as a trade mark, from B. subtilis natto fermentation. In the present study, we examined the fibrinolytic and antithrombotic effects of NKCP and identified its active component to clarify the fibrinolytic effect of NKCP observed in preliminary clinical trials previously. The active component of NKCP was identified as a 34-kilodalton protein designated bacillopeptidase F. NKCP showed direct degradation of artificial blood clot in saline. The protease activity, accounting for the fibrinolytic effect of NKCP, was examined with a chromogenic substrate for plasmin. Dose-dependent prolongations of both prothrombin time and active partial thromboplastin time were observed in rats with intra-duodenum administration of NKCP. Our in vitro and in vivo studies suggest that NKCP has both a fibrinolytic effect and an antithrombotic effect similar to heparin. Because NKCP is derived from food and has safety data demonstrated by previous animal experiments and preliminary clinical trials, NKCP is considered as safe for clinical use.

  8. Production and Purification of Pharmaceutically Important Fibrinolytic Enzyme from Bacillus Species

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    Sharav A. Desai

    2015-11-01

    Full Text Available The medicinal and pharmaceutical importance of currently available thrombolytic agents like urokinase, t-PA, streptokinase, staphylokinase and others, demonstrated repeatedly since 1970s, however sometimes they cause undesirable side effects like bleeding and allergic responds. The present findings reports isolation, screening and identification of soil bacterium for production of fibrinolytic enzyme. Samples for the study were collected from different locations were first screened for proteolytic activity using skimmed milk agar plate and lastly fibrin plate method was used to evaluate fibrinolytic activity. The strain capable of producing fibrinolytic protein was identified as Bacillus Spp. Using both Bergery’s manual of systemic bacteriology and biochemical characterization simultaneously. Selected strain was than subjected to the process of fermentation using basal media for 5 days, 37°C and at 180rpm. Protein content and fibrinolytic activity were measured by Biuret method using bovine serum albumin as standard and fibrinolytic assay respectively. Three stage purification was done, that includes salting out with ammonium sulphate, followed by gel filtration chromatography and finally separated by RP-HPLC, proteins were eluted in peaks with a retention time of 2.092, 3.188, 5.178, 7.295, and 11.32 minutes. The fraction with retention time 7.295 minutes shows a maximum activity. The enzyme found to be having an optimum pH between 7.0 and 7.5. Enzyme is also stable at the optimum pH and found to lose its activity on higher side of acidity or alkalinity. It is more active at 40°C and is stable at 37°C to 43°C with slight modification in activity.

  9. cDNA cloning and expression of earthworm fibrinolytic enzyme component A

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Earthworm fibrinolytic enzyme component A (EFEa), a protein with dual fibrinolytic activity, is one of the major therapeutically important earthworm fibrinolytic enzyme components. The cDNA fragment encoded the mature protein was cloned from earthworm (Eisenia fetida) by the RT-PCR technique. The deduced amino acid sequence of the EFE component A shows high homology with some members of serine proteases trypsin family, and the amino acid residues constituting the active sites are conserved in the EFEa as compared with the other proteins of the trypsin family. The cDNA fragment was subcloned into the expression vector pQE31 and pMAL-c2X of E. coli. The resulting expression plasmids, pQE-efea and pMAL-efea, were used to transform the E. coli strain M15. Recombinant protein bands corresponding with calculated molecular weights were induced. The induced His6-EFEa fusion protein with pQE- efea was accumulated into inclusion body, while the induced MBP-EFEa fusion protein with pMAL-efea was soluble and showed fibrinolytic activities.

  10. Association among Fibrinolytic Proteins, Metabolic Syndrome Components, Insulin Secretion, and Resistance in Schoolchildren

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    Jin-Shuen Chen

    2015-01-01

    Full Text Available We investigated the role of urokinase plasminogen activator (uPA and its soluble receptors (suPAR and plasminogen activator inhibitor-1 (PAI-1 in metabolic syndrome (MetS components, insulin secretion, and resistance in schoolchildren. We enrolled 387 children, aged 10.3 ± 1.5 years, in Taipei. Anthropometry, fibrinolytic proteins, MetS components, insulin secretion, and resistance were measured. Subjects were divided into normal, overweight, and obese groups. Finally, the relationship between fibrinolytic proteins and metabolic syndrome in boys and girls was analyzed. In boys, PAI-1 was positively associated with body mass index (BMI percentile, hypertriglyceride, insulin secretion, and resistance. In girls, PAI-1 was positively associated with obesity, hypertriglyceridemia, and insulin secretion. In girls, uPA was positively associated with insulin secretion. suPAR was positively associated with high-sensitivity C-reactive protein in both boys and girls, and with BMI percentile and body fat in girls. The obese boys had higher suPAR and PAI-1 levels than the normal group. The obese girls had higher uPA, suPAR, and PAI-1 than the normal group. Boys and girls with MetS had higher PAI-1. Fibrinolytic proteins, especially PAI-1, are associated with MetS components and insulin secretion in children. Fibrinolytic proteins changes were more likely to occur in girls than in boys.

  11. Evaluation of fibrinolytic and antioxidant effects of Allium elburzense bulb extracts

    Directory of Open Access Journals (Sweden)

    Leila Safaeian

    2017-04-01

    Full Text Available Objective: Allium elburzense is an endemic plant of the family Amaryllidaceae that grows wild in northern Iran with some nutritional and medicinal applications. The present study was aimed to investigate the fibrinolytic and antioxidant effects of A. elburzense bulb extracts. Materials and Methods: Hydroalcoholic, aqueous, chloroformic and butanolic extracts were evaluated in this research. In vitro antioxidant assays were performed using total phenolic, DPPH, and FRAP methods. In the in vivo analysis, animals receivedi.p. injection of A. elburzense hydroalcoholic extract for 21 days and hydroperoxides level, FRAP value, PT and aPTT were determined in serum samples. The fibrinolytic activity of different extracts was quantitatively evaluated by measurement of clot weight. Results: In vitro antioxidant assay showed that A. elburzense aqueous extract had the highest DPPH scavenging and the highest total antioxidant capacity. In the in vivo assay, A. elburzense hydroalcoholic extract reduced serum hydroperoxides level and increased serum total antioxidant capacity in rats.In vitro fibrinolytic assay revealed remarkable thrombolytic activity for this plant with the highest effect for the aqueous extract. However, coagulation parameters including PT and aPTT were not affected by administration of A. elburzense hydroalcoholic extract in rats. Conclusion: In conclusion, the results of this study revealed the potential antioxidant and fibrinolytic effects of A. elburzense bulbextracts. For developing novel thrombolytic agents, further investigations for isolation of bioactive constituents and finding the underlying mechanisms are suggested.

  12. Effect of a single dose of mesoglycan on the human fibrinolytic system, and the profibrinolytic action of nine daily doses.

    Science.gov (United States)

    Vittoria, A; Messa, G L; Frigerio, C; Celasco, G; Auteri, A

    1988-01-01

    The profibrinolytic activity of orally administered Mesoglycan was evaluated in 18 patients affected by impaired plasma fibrinolytic activity. Mesoglycan was administered by a single oral dose of 24, 48 or 72 mg on 1 day, and by repeated doses of 48 mg twice a day for 9 consecutive days. After the single administration all the fibrinolytic parameters were significantly and positively influenced with an order of magnitude and a duration of effects proportional to the dose employed. After the repeated administration, a constant and reproducible activation of the fibrinolytic system was observed without any interference with haemocoagulative parameters. These results confirm that Mesoglycan is endowed with a relevant profibrinolytic activity in man after oral administration. The pharmacological activity of Mesoglycan could possibly involve the liberation of a certain amount of plasminogen tissue activator.

  13. Role of mesenchymal stem cell-derived fibrinolytic factor in tissue regeneration and cancer progression.

    Science.gov (United States)

    Heissig, Beate; Dhahri, Douaa; Eiamboonsert, Salita; Salama, Yousef; Shimazu, Hiroshi; Munakata, Shinya; Hattori, Koichi

    2015-12-01

    Tissue regeneration during wound healing or cancer growth and progression depends on the establishment of a cellular microenvironment. Mesenchymal stem cells (MSC) are part of this cellular microenvironment, where they functionally modulate cell homing, angiogenesis, and immune modulation. MSC recruitment involves detachment of these cells from their niche, and finally MSC migration into their preferred niches; the wounded area, the tumor bed, and the BM, just to name a few. During this recruitment phase, focal proteolysis disrupts the extracellular matrix (ECM) architecture, breaks cell-matrix interactions with receptors, and integrins, and causes the release of bioactive fragments from ECM molecules. MSC produce a broad array of proteases, promoting remodeling of the surrounding ECM through proteolytic mechanisms. The fibrinolytic system, with its main player plasmin, plays a crucial role in cell migration, growth factor bioavailability, and the regulation of other protease systems during inflammation, tissue regeneration, and cancer. Key components of the fibrinolytic cascade, including the urokinase plasminogen activator receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1), are expressed in MSC. This review will introduce general functional properties of the fibrinolytic system, which go beyond its known function of fibrin clot dissolution (fibrinolysis). We will focus on the role of the fibrinolytic system for MSC biology, summarizing our current understanding of the role of the fibrinolytic system for MSC recruitment and the functional consequences for tissue regeneration and cancer. Aspects of MSC origin, maintenance, and the mechanisms by which these cells contribute to altered protease activity in the microenvironment under normal and pathological conditions will also be discussed.

  14. Markers of fibrinolytic potency and clotting activation in stable angina pectoris : Role of urokinase, assessment of atrioventricular differences and correlation with coronary patency

    NARCIS (Netherlands)

    Páramo, U.A.; Panizo, C.; Montes, R.; Orbe, J.; Alegría, E.; Martínez-Caro, D.; Dooijewaard, G.

    1999-01-01

    To characterize the extent of activation of the hemostatic system and to detect local alterations which may favour thrombus formation we obtained samples from the right atrium (RA) and left ventricle (LV) of 60 stable angina patients (mean age 59 years, M/F: 49/11) during the cardiac catheterization

  15. Influence of PAI-1 gene promoter-675 (4G/5G) polymorphism on fibrinolytic activity after cardiac surgery employing cardiopulmonary bypass.

    Science.gov (United States)

    Ozolina, Agnese; Strike, Eva; Jaunalksne, Inta; Serova, Jelena; Romanova, Tatjana; Zake, Liene Nikitina; Sabelnikovs, Olegs; Vanags, Indulis

    2012-01-01

    The plasminogen activator inhibitor type-1 (PAI-1) gene promoter contains 675 (4G/5G) polymorphism. The aim of this study was evaluate the effect of the PAI-1 promoter-675 (4G/5G) polymorphism on the concentrations of PAI-1 and tissue plasminogen activator/PAI-1 (t-PA/PAI-1) complex and bleeding volume after on-pump cardiac surgery. A total of 90 patients were included in the study at Pauls Stradins Clinical University Hospital. Seven patients were excluded due to surgical bleeding. Eighty-three patients were classified according to the PAI-1 genotype: 21 patients had the 4G/4G genotype; 42, the 4G/5G genotype; and 20, the 5G/5G genotype. The following fibrinolysis parameters were recorded: the PAI-1 level preoperatively, D-dimer level at 0, 6, and 24 hours after surgery, and t-PA/PAI-1 complex level 24 hours postoperatively. A postoperative bleeding volume was registered in mL 24 hours after surgery. The patients with the 5G/5G genotype had significantly lower preoperative PAI-1 levels (17 [SD, 10.8] vs. 24 ng/mL [SD, 9.6], P=0.04), higher D-dimer levels at 6 hours (371 [SD, 226] vs. 232 ng/mL [SD, 185], P=0.03) and 24 hours (326 [SD, 207] vs. 209 ng/mL [SD, 160], P=0.04), and greater postoperative blood loss (568 [SD, 192] vs. 432 mL [168], P=0.02) compared with the 4G/4G carriers. There were no significant differences in the levels of the t-PA/PAI-1 complex comparing different genotype groups. The carriers of the 5G/5G genotype showed the lower preoperative PAI-1 levels, greater chest tube blood loss, and higher D-dimer levels indicating that the 5G/5G carriers may have enhanced fibrinolysis.

  16. Isolation and identification of a novel fibrinolytic Bacillus tequilensis CWD-67 from dumping soils enriched with poultry wastes.

    Science.gov (United States)

    Velusamy, Palaniyandi; Pachaiappan, Raman; Christopher, Meera; Vaseeharan, Baskaralingam; Anbu, Periasamy; So, Jae-Seong

    2015-01-01

    A newly isolated strain, CWD-67, which exhibited high fibrinolytic activity, was screened from dumping soils enriched with poultry wastes. The strain was identified as Bacillus tequilensis (KF897935) by 16Sr RNA gene sequence analysis and biochemical characterization. A fibrinolytic enzyme was purified to homogeneity from the culture supernatant using ammonium sulfate precipitation, membrane concentration, dialysis, ion-exchange, and gel filtration chromatography. SDS-PAGE analysis showed that the purified enzyme was a monomeric protein with an apparent molecular weight of 22 kDa, which is the lowest among Bacillus fibrinolytic enzymes reported to date. The purified enzyme was confirmed to have fibrinolytic activity by a fibrin zymogram. The optimal pH and temperature values of the enzyme were 8.0 and 45 °C, respectively. The enzyme was completely inhibited by PMSF and significantly inhibited by EDTA, TPCK, Co(2+), Zn(2+), and Cu(2+), suggesting a chymotrypsin-like serine metalloprotease. In vitro assays revealed that the purified enzyme could catalyze fibrin lysis effectively, indicating that this enzyme could be a useful fibrinolytic agent.

  17. Effects of lycopene on blood cells and fibrinolytic activity in hyperlipidemic rats%番茄红素对高脂血症大鼠血细胞和纤溶活性的影响

    Institute of Scientific and Technical Information of China (English)

    许晓丽; 朱密; 胡敏予

    2011-01-01

    目的 探讨番茄红素对高脂血症大鼠血细胞及纤溶活性的影响.方法 成年雄性SD大鼠40只,根据总胆固醇(TC)水平随机分为5组,每组8只:正常对照组、高脂模型组、氟伐他汀钠10mg/kg bw组、番茄红素11 mg/kg bw和44mg/kg bw组.正常对照组饲基础饲料,其余组饲高脂饲料,实验第2、3周氟伐他汀钠和番茄红素灌胃处理.检测TC、甘油三酯(TG)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、超氧化物歧化酶活力(SOD)和丙二醛水平(MDA)、血细胞参数、血小板α颗粒膜蛋白(GMP-140)、组织型纤溶酶原激活物(tPA)、组织型纤溶酶原激活物抑制剂-1(PAI-1),计算tPA/PAI-1比值和动脉粥样硬化指数(AI);HE染色观察主动脉弓的病理变化.结果 高脂饲料喂养1周后大鼠形成高脂血症.与模型组比较,番茄红素组的TC、TG、LDL-C和MDA下降,SOD升高;白细胞计数、红细胞体积、血小板体积、GMP-140和PAI-1下降,红细胞计数和tPA上升;AI显著下降,主动脉弓内膜变薄,泡沫细胞减少.番茄红素44mg/kg bw剂量组作用明显.结论 番茄红素可能通过降低血脂和抗氧化而保护高脂血症大鼠的血细胞和促进纤溶活性,减轻主动脉病变程度.%Objective To explore the effects of lycopene on blood cells and fibrinolytic activity in hyperlipidemic rats. Methods Forty adult male SD rate were divided randomly into five groups; normal control group, high fat model group, fluvastatin sodium group, lycopene low-dose group and lycopene high-dose group based on the level of serum TC. Normal control group was fed with normal animal diet, other groups were fed with high fat diet. Fluvastatin sodium and lycopene were given by intragastric administration in the last two weeks of experiment. The level of serum lipid, SOD, MDA, blood cell parameters, platelet (granule membrane protein (CMP-140), tissue plasminogen activator ( t-PA) and plasminogen activator

  18. A Fibrinolytic Enzyme Produced by Bacillus subtilis Using Chickpea (Cicer arietinum L. as Substrate

    Directory of Open Access Journals (Sweden)

    Ping Xiao

    2014-12-01

    Full Text Available A Fibrinolytic Enzyme (BSFE was isolated from fermented chickpeas using Bacillus subtilis. BSFE was purified with ammonium sulfate precipitation, ion exchange and gel filtration chromatography. The fibrin (ogen olytic activity of BSFE was investigated by means of fibrinolysis plate and hydrolysis of fibrinogen. Through these steps, the purity of the enzyme increased with 74.60-fold with 6.88% recovery activity. The molecular weight of the BSFE was estimated to be 30 kDa by SDS-PAGE. The optimum pH, optimum temperature, pH stability and thermal stability of BSFE were measured, respectively as 8.0, 55°C, 6.0-8.0 and less than 45°C. The activity was inhibited by serine protease inhibitor PMSF as well as metalloprotease inhibitor EDTA, indicating that the BSFE is a serine metalloprotease. In fibrin plate assay, BSFE showed more stronger fibrinolytic activity than that of nattokinase and it specifically hydrolyzed Aα and Bβ chains followed by γ chain of fibrinogen. Therefore, this study provided a method and it for the preparation of multifunctional food of chickpeas which has strong fibrinolytic activity.

  19. Purification and Characterisation of a Fibrinolytic Enzyme from Rhizopus micro sporus var. tuberosus

    Directory of Open Access Journals (Sweden)

    Shuli Zhang

    2015-01-01

    Full Text Available Extracellular fibrinolytic enzyme from Rhizopus microsporus var. tuberosus was purified and characterised. The microorganism was isolated in a distillery from daqu, a fermentative agent used in the production of Chinese liquor and vinegar at diff erent temperatures. The fibrinolytic enzyme was partially purifi ed by ammonium sulphate precipitation, dialysis, DEAE Sepharose® Fast Flow ion exchange chromatography and Sephadex G-75 gel filtration chromatography. The molecular mass of the fi brinolytic enzyme was estimated to be 24.5 kDa by SDS-PAGE. The purified enzyme showed optimal activity at pH=7.0 and 37 °C by fibrin plate method. It showed stronger resistance to the inhibition by trypsin and was stable at 37 °C retaining 96.1 % residual activity aft er 4 h of incubation. The fibrinolytic activity of the enzyme was enhanced by Na+, Ca2+, Mg2+ and Mn2+. Conversely, Zn2+ and Cu2+ partly inhibited enzymatic activity. Using fibrin plate method, we found that the enzyme not only degrades fibrin directly, but also activates plasminogen into plasmin to degrade fibrin. The results indicate that the pure enzyme has a potential in dissolving blood clot, and the possibility for application in the treatment of thrombosis.

  20. The fibrinolytic system: A new target for treatment of depression with psychedelics.

    Science.gov (United States)

    Idell, R D; Florova, G; Komissarov, A A; Shetty, S; Girard, R B S; Idell, S

    2017-03-01

    Current understanding of the neurobiology of depression has grown over the past few years beyond the traditional monoamine theory of depression to include chronic stress, inflammation and disrupted synaptic plasticity. Tissue plasminogen activator (tPA) is a key factor that not only promotes fibrinolysis via the activation of plasminogen, but also contributes to regulation of synaptic plasticity and neurogenesis through plasmin-mediated activation of a probrain derived neurotrophic factor (BDNF) to mature BDNF. ProBDNF activation could potentially be supressed by competition with fibrin for plasmin and tPA. High affinity binding of plasmin and tPA to fibrin could result in a decrease of proBDNF activation during brain inflammation leading to fibrosis further perpetuating depressed mood. There is a paucity of data explaining the possible role of the fibrinolytic system or aberrant extravascular fibrin deposition in depression. We propose that within the brain, an imbalance between tPA and urokinase plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) and neuroserpin favors the inhibitors, resulting in changes in neurogenesis, synaptic plasticity, and neuroinflammation that result in depressive behavior. Our hypothesis is that peripheral inflammation mediates neuroinflammation, and that cytokines such as tumor necrosis factor alpha (TNF-α) can inhibit the fibrinolytic system by up- regulating PAI-1 and potentially neuroserpin. We propose that the decrement of the activity of tPA and uPA occurs with downregulation of uPA in part involving the binding and clearance from the surface of neural cells of uPA/PAI-1 complexes by the urokinase receptor uPAR. We infer that current antidepressants and ketamine mitigate depressive symptoms by restoring the balance of the fibrinolytic system with increased activity of tPA and uPA with down-regulated intracerebral expression of their inhibitors. We lastly hypothesize that psychedelic 5-ht2a receptor agonists

  1. Role of excess glycogenolysis in fasting hyperglycemia among pre-diabetic and diabetic Zucker (fa/fa) rats.

    Science.gov (United States)

    Jin, Eunsook S; Park, Byung-Hyun; Sherry, A Dean; Malloy, Craig R

    2007-03-01

    Sources of plasma glucose and glucose turnover were investigated in 8-week-old (pre-diabetic) and 13-week-old (diabetic) Zucker (fa/fa) rats after a 24-h fast. Intraperitoneal (2)H(2)O was administered and [3,4-(13)C(2)]glucose and [U-(13)C(3)]propionate were infused into conscious active rats. (13)C nuclear magnetic resonance analysis of monoacetone glucose derived from blood glucose indicated that glucose production was increased significantly in 8- and 13-week-old fa/fa rats compared with age-matched Zucker (+/+) rats, and hepatic glycogen was dramatically higher among fa/fa animals regardless of age. Glycogenolysis, essentially 0 in +/+ rats after a 24-h fast, was significant in fa/fa rats (11 +/- 6 and 17 +/- 7% of glucose production in 8- and 13-week-old rats, respectively), even after a 24-h fast. Tricarboxylic acid (TCA) cycle flux and efflux of carbon skeletons from the cycle (cataplerosis) were both significantly higher in fa/fa rats compared with controls, but net gluconeogenesis from the TCA cycle was not higher because products leaving the cycle were returned to the cycle via a pyruvate cycling pathway. Thus, pyruvate cycling flux increased in proportion to TCA cycle flux, leaving net gluconeogenesis unchanged in fa/fa animals compared with control animals. The distribution of (2)H in skeletal muscle glycogen suggested that at least a fraction of glucose molecules entering glycogen pass through phosphomannose isomerase.

  2. In vitro decreases of the fibrinolytic potential of cultured human fibrosarcoma cell line, HT1080, by Nigella sativa oil.

    Science.gov (United States)

    Awad, E M

    2005-01-01

    It is generally accepted that the fibrinolytic potential of tumor cells is related to their malignant phenotype. In the present study, Nigella sativa oil (NSO) was studied to evaluate its effect on the fibrinolytic potential of the fibosarcoma cell line HT1080 to elucidate whether this oil might have an antitumor activity through its modulation of the fibrinolytic potential of such cells. NSO produced a concentration-dependent inhibition of tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA) and plasminogen activator inhibitor type 1 (PAI-1). When subconfluent HT1080 cells were conditioned with oil, a concentration (0.0-200 microg oil/ml)-dependent decrease in t-PA, u-PA and PAI-1 antigen was observed. There was also a concentration-dependent decrease (from 0.0 to 112.5 microg oil/ml) in the confluent cultures. The results showed that blackseed oil decreases the fibrinolytic potential of the human fibrosarcoma cell line (HT1080) in vitro, implying that inhibition of local tumor invasion and metastasis may be one such mechanism.

  3. Enzymatic properties of UFE,a novel marine fibrinolytic enzyme

    Institute of Scientific and Technical Information of China (English)

    WANG Dianliang; LIU Wanshun; HAN Baoqin

    2007-01-01

    A novel potent protease, Urechis unicinctus fibrinolytic enzyme (UFE), was firstly discovered. The enzymatic properties of UFE were further investigated.As a low molecular mass protein,UFE appeared to be very stable to heat and pH.When temperature was below 50 °C ,the remnant enzyme activity remained almost unchanged, but when temperature was raised to 60 °C ,the remnant enzyme activity began to decrease rapidly. UFE was quite stable in the range of pH value from 3 to 12,especially in slightly alkaline pH value.Mn2+ ,Cu2+ and Fe2+ ions were activators of UFE, while Fe3+ and Ag+ ions were inhibitors of UFE.Fe2+ ion along with Fe3+ ion might regulate UFE activity in vivo. The optimum pH and temperature of UFE were about 8 and 50 °C ,respectively. Other characteristics of this enzyme were also studied. Systematic research results are significant when UFE is applied for medical and industrial purposes.

  4. Global fibrinolytic capacity in pediatric patients with sepsis and disseminated intravascular coagulation.

    Science.gov (United States)

    Bay, Ali; Oner, Ahmet Faik; Kose, Dogan; Dogan, Murat

    2006-10-01

    There are many complex pathophysiologic changes of the coagulation system in sepsis. The fibrinolytic system was evaluated in septic children using the global fibrinolytic capacity (GFC), a new technique reflecting the overall fibrinolytic activity. The study consisted of 24 children with sepsis, 36 children with sepsis plus disseminated intravascular coagulation (DIC), and 20 healthy age-matched control individuals. Compared with controls, 86% of sepsis patients and 87% of sepsis plus DIC patients had decreased GFC levels. Between the sepsis plus DIC and sepsis groups there was no significant difference in terms of GFC levels. While 19 patients (52.7%) died in the sepsis plus DIC group, only three patients (12.5%) died in the sepsis group. When survivors and nonsurvivors were compared in terms of coagulation tests, there were significant differences for protein C, antithrombin, platelet, fibrinogen, aspartate aminotransferase, alanine aminotransferase, prothrombin time, and white blood cell values. In conclusion, the level of GFC reduced in most of the pediatric sepsis patients but no difference was observed between patients with sepsis and patients with sepsis plus DIC. While inhibition of fibrinolysis is an important finding in sepsis, the mortality is mainly associated with the presence of end-organ damage and the status of coagulation parameters.

  5. Enhancement of protease production by Pseudomonas aeruginosa isolated from dairy effluent sludge and determination of its fibrinolytic potential

    Institute of Scientific and Technical Information of China (English)

    Amrita Raj; Nancy Khess; Namrata Pujari; Sourav Bhattacharya; Arijit Das; Subbaramiah Sundara Rajan

    2012-01-01

    Objective: The present study aimed at isolating proteolytic bacteria from dairy effluent sludge, designing the process parameters for the enhanced production of protease and determination of its fibrinolytic potential. Methods: The dairy sludge was processed according to the microbiological criteria for the isolation of proteolytic bacteria. All the isolates were screened for their protease production ability and the isolate showing highest proteolysis was selected for further studies. Effects of various media components and process parameters like carbon and nitrogen supplementation, temperature, pH and incubation period were investigated. Partial purification of the protease was done using ammonium sulphate fractionation, following which its molecular weight and fibrinolytic activity were determined. Results: Based on the biochemical studies, the selected isolate was identified as Pseudomonas aeruginosa. The highest protease yield was obtained with maltose and yeast extract as supplements. The optimum pH, temperature and incubation period for protease production by the isolate was found to be 7.0, 37℃ and 48 h respectively. The partially purified enzyme preparation showed a single protein band in sodium dodecyl sulphate polyacrylamide gel electrophoresis, revealing the apparent molecular weight of the enzyme to be 35 kDa. The efficient removal of the blood stain emphasized its fibrinolytic potential. Conclusions: From the present study it is envisaged that cultural parameters significantly affect the protease production. Based upon the fibrinolytic activity, this protease may find broad applications in detergent and pharmaceutical industries.

  6. Purification and characterization of fibrinolytic metalloprotease from Perenniporia fraxinea mycelia.

    Science.gov (United States)

    Kim, Jae-Sung; Kim, Ji-Eun; Choi, Bong-Suk; Park, Se-Eun; Sapkota, Kumar; Kim, Seung; Lee, Hyun-Hwa; Kim, Chun-Sung; Park, Yeal; Kim, Myung-Kon; Kim, Yoon-Sik; Kim, Sung-Jun

    2008-08-01

    In this study we purified and characterized a fibrinolytic protease from the mycelia of Perenniporia fraxinea. The apparent molecular mass of the purified enzyme was estimated to be 42kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), fibrin zymography and size exclusion using fast protein liquid chromatography (FPLC). The first 20 amino acid residues of the N-terminal sequence were ASYRVLPITKELLPPEFFVA, which shows a high degree of similarity with a fungalysin metallopeptidase from Coprinopsis cinerea. The optimal reaction pH value and temperature were pH 6.0 and 35-40 degrees C, respectively. Results for the fibrinolysis pattern showed that the protease rapidly hydrolyzed the fibrin alpha-chain followed by the beta-chain. The gamma-gamma chains were also hydrolyzed, but more slowly. The purified protease effectively hydrolyzed fibrinogen, preferentially digesting the Aalpha-chains of fibrinogen, followed by Bbeta- and gamma-chains. We found that protease activity was inhibited by Cu(2+), Fe(3+), and Zn(2+), but enhanced by the additions of Mn(2+), Mg(2+) and Ca(2+) metal ions. Furthermore, the protease activity was inhibited by EDTA, and it was found to exhibit a higher specificity for the chromogenic substrate S-2586 for chymotrypsin, indicating that the enzyme is a chymotrypsin-like metalloprotease. The mycelia of P. fraxinea may thus represent a source of new therapeutic agents to treat thrombosis.

  7. Fibrinolytic therapy for mechanical pulmonary valve thrombosis.

    Science.gov (United States)

    Khajali, Zahra; Mohammadzadeh, Shabnam; Maleki, Majid; Peighambari, Mohammad Mehdi; Sadeghpoor, Anita; Ghavidel, Alireza; Elahi, Behrad; Mirzaaghayan, Mohammadreza

    2015-01-01

    Treatment of prosthetic heart valve thrombosis using intravenous thrombolytics, although an acceptable alternative to surgery, is not complication free, and the literature has a dearth of data on the subject. This study analyzed the results of fibrinolytic treatment (FT) among a single-center group of patients with mechanical pulmonary valve thrombosis. Between 2000 and 2013, 23 consecutive patients with 25 episodes of pulmonary valve thrombosis received FT. The diagnosis of mechanical pulmonary valve thrombosis was established by fluoroscopy and echocardiography. Streptokinase (SK) was used in 24 cases and alteplase in 1 case. The FT was continued a second day for 14 patients (58.3%), a third day for 1 patient, and a fourth day for 1 patient. Echocardiography and fluoroscopy were performed every day until improvement of malfunction was achieved. Of the 23 patients, 19 had complete resolution of hemodynamic abnormalities after FT, 1 had partial resolution, and 2 showed no change. No patient had major complications. Five minor complications were detected, namely, fever, nausea, thrombophlebitis, epistaxi, and pain. Seven patients (30%) experienced recurrence of thrombosis, whereas four patients had surgery (biological pulmonary valve replacement) without re-thrombolytic therapy, one patient was treated with Alteplase, one patient received SK, and one patient received intense anticoagulation using heparin and warfarin. Overall, FT had a success rate of 84%. The results indicate that regardless of the time to pulmonary valve replacement and echocardiographic and fluoroscopic findings, FT was effective in most cases of mechanical pulmonary valve thrombosis. The efficacy increased with second-day thrombolytic therapy. Major complications were not common after lytic therapy for mechanical pulmonary valve thrombosis.

  8. Characterization of a Novel Fibrinolytic Enzyme, BsfA, from Bacillus subtilis ZA400 in Kimchi Reveals Its Pertinence to Thrombosis Treatment.

    Science.gov (United States)

    Ahn, Min-Ju; Ku, Hye-Jin; Lee, Se-Hui; Lee, Ju-Hoon

    2015-12-28

    Recently, the cardiovascular disease has been widely problematic in humans probably due to fibrin formation via the unbalanced Western style diet. Although direct (human plasmin) and indirect methods (plasminogen activators) have been available, bacterial enzyme methods have been studied because of their cheap and mass production. To detect a novel bacterial fibrinolytic enzyme, 111 bacterial strains with fibrinolytic activity were selected from kimchi. Among them, 14 strains were selected because of their stronger activity than 0.02 U of plasmin. Their 16S rRNA sequence analysis revealed that they belong to Bacillus, Leuconostoc, Propionibacterium, Weissella, Staphylococcus, and Bifidobacterium. The strain B. subtilis ZA400, with the highest fibrinolytic activity, was selected and the gene encoding fibrinolytic enzyme (bsfA) was cloned and expressed in the E. coli overexpression system. The purified enzyme was analyzed with SDS-PAGE, western blot, and MALDI-TOF analyses, showing to be 28.4 kDa. Subsequently, the BsfA was characterized to be stable under various stress conditions such as temperature (4-40°C), metal ions (Mn(2+), Ca(2+), K(2+), and Mg(2+)), and inhibitors (EDTA and SDS), suggesting that BsfA could be a good candidate for development of a novel fibrinolytic enzyme for thrombosis treatment and may even be useful as a new bacterial starter for manufacturing functional fermented foods.

  9. The binding interactions of imidacloprid with earthworm fibrinolytic enzyme

    Science.gov (United States)

    Wang, Yan-Qing; Zhang, Hong-Mei; Chen, Tao

    2014-08-01

    In this paper, several studies were conducted to elucidate the binding mechanism of earthworm fibrinolytic enzyme (EFE) with imidocloprid (IMI) by using theoretical calculation, fluorescence, UV-vis, circular dichroism spectroscopy and an enzymatic inhibition assay. The spectral data showed that the binding interactions existed between IMI and EFE. The binding constants, binding site, thermodynamic parameters and binding forces were analyzed in detail. The results indicate a single class of binding sites for IMI in EFE and that this binding interaction is a spontaneous process with the estimated enthalpy and entropy changes being 2.195 kJ mol-1 and 94.480 J mol-1 K-1, respectively. A single class of binding site existed for IMI in EFE. The tertiary or secondary structure of EFE was partly destroyed by IMI. The visualized binding details were also exhibited by the theoretical calculation and the results indicated that the interaction between IMI and Phe (Tyr, or Trp) or EFE occurred. Combining the experimental data with the theoretical calculation data, we showed that the binding forces between IMI and EFE were mainly hydrophobic force accompanied by hydrogen binding, and π-π stacking. In addition, IMI did not obviously influence the activity of EFE. In a word, the above analysis offered insights into the binding mechanism of IMI with EFE and could provide some important information for the molecular toxicity of IMI for earthworms.

  10. Fibrinolytic agents in the management of posthemorrhagic hydrocephalus in preterm infants: the evidence.

    Science.gov (United States)

    Haines, S J; Lapointe, M

    1999-05-01

    The objective of this study was to review current literature on the management of posthemorrhagic hydrocephalus in preterm infants with intraventricular administration of fibrinolytic agents; to this end a literature search was carried out electronically. The keywords used were "intraventricular hemorrhage" or "posthemorrhagic hydrocephalus" in combination with "fibrinolytic agent," "urokinase," "streptokinase," or "recombinant tissue plasminogen activator" and "intraventricular administration"; the search covered the years 1966-1998 and was restricted to English language papers and human subjects. It was supplemented by a search through the reference lists of the articles identified. Articles dealing with intracerebral hemorrhage or hematoma, intraventricular hemorrhage in adults, nontherapeutic issues and laboratory research were excluded. The articles included are summarized in evidence and evaluation tables. Five scientific publications evaluating the use of a fibrinolytic agent to manage posthemorrhagic hydrocephalus were retrieved. In the studies described in these reports, a total of 62 neonates received streptokinase, urokinase or r-tPA intraventricularly. No two of the regimens were identical in the drug used, method of administration and duration of therapy. The time before therapy was started ranged from 2 to 35 days after the ictus. Among the case series reported, three were small series with a total of 38 neonates. One other case series of 18 neonates compared the treatment group with an historical control group. All case series showed that endoventricular fibrinolytic therapy was practical. The proportion of cases in which shunt placement was performed ranged from 11% to 100%. Only one small prospective, randomized, controlled study was identified. That study was too small to allow useful conclusions. Overall, 3 cases of secondary intraventricular hemorrhage were reported. However, it was not possible to determine with certainty whether these episodes

  11. Disturbance of vasodilation via protease-activated receptor 2 in SHRSP.Z-Lepr fa/IzmDmcr rats with metabolic syndrome.

    Science.gov (United States)

    Kagota, Satomi; Maruyama, Kana; Wakuda, Hirokazu; McGuire, John J; Yoshikawa, Noriko; Nakamura, Kazuki; Shinozuka, Kazumasa

    2014-10-01

    Protease-activated receptor-2 (PAR2) activation causes vascular inflammation and vasodilation, but its role in metabolic syndrome (MetS) remains uncertain. Therefore, we examined whether the PAR2-induced vasodilation of SHRSP.Z-Lepr(fa)/IzmDmcr rats (SHRSP.ZF) is impaired and if so, whether administering telmisartan is protective. PAR2-activating peptide, 2-furoyl-LIGRLO-amide (2fly), relaxed the isolated superior and first-order branches of mesenteric arteries (MAs) from Wistar-Kyoto rats (WKY) and SHRSP.ZF. Superior-MA relaxation by 2fly was less in SHRSP.ZF than in WKY. Relaxation of first-order MAs by 2fly was the same in SHRSP.ZF and WKY. NO synthase inhibitor partially reduced 2fly-induced relaxation of superior and first-order MAs in SHRSP.ZF and WKY; inhibition of relaxation was proportionately larger in SHRSP.ZF. In SHRSP.ZF, nitroprusside-induced relaxation and the expression of soluble guanylyl cyclase decreased. In SHRSP.ZF, telmisartan reversed these abnormalities, and decreased blood pressure and serum levels of thiobarbituric acid reactive substances, an index of oxidative stress. Vasodilation via PAR2 activation was preserved in small-caliber MAs, in contrast to large-caliber MAs, even when MetS reduced NO-dependent relaxation mechanisms. NO and non-NO relaxing factor(s) contributed to PAR2-mediated relaxation in MAs, and the balance between factors may be altered to preserve vasodilation in MetS. Telmisartan prevented vascular dysfunction in MetS by protecting arteries against oxidative stress.

  12. Biochemical characterization of a novel fibrinolytic enzyme from Cordyceps militaris.

    Science.gov (United States)

    Liu, Xiaolan; Kopparapu, Narasimha-Kumar; Li, Yao; Deng, Yongping; Zheng, Xiqun

    2017-01-01

    A fibrinolytic enzyme was produced by the medicinal mushroom, Cordyceps militaris using submerged fermentation. The enzyme was purified from culture supernatant by hydrophobic interaction, ion exchange and gel filtration chromatographies. It was purified by 36 fold, with a specific activity of 1,467.4U/mg protein and the final yield was 5.8%. The molecular weight of the enzyme as determined by SDS-PAGE and gel filtration was 28kDa and 24.5kDa, respectively, and its isoelectric point (pI) was 9.0±0.2. It was found to be a glycoprotein with carbohydrate content of 1.67% (w/v). The enzyme was optimally active at 37°C and pH 7.2. The enzyme activity was strongly inhibited by soybean trypsin inhibitor (SBTI) and aprotinin which indicated it to be a serine protease, while other inhibitors like N-α-tosyl-l-phenylalanine chloromethyl ketone (TPCK), phenyl methane sulfonyl fluoride (PMSF), pepstatin and metal chelator EDTA did not inhibit its activity. Amino acid sequences of the purified enzyme were determined partially by Q-TOF2 and they were IEDFPYQVDLR; ANCGGTVISEK; YVLTAGHCAEGYTGLNIR; TNYASVTPITADMICAGFPEGK; KDSCSGDSGGPLVTGGK; VVGIVSFGTGCAR; ANKPGVYSSVASAEIR. Sequences of the seven peptides completely matched with those of a trypsin-like serine protease from Cordyceps militaris CM01 (accession no. EGX95217.1). The purified enzyme degraded α chains of fibrinogen first and then β and γ chains and also activated plasminogen into plasmin. It can act as an anticoagulant and prevent clot formation by degrading fibrinogen. Based on these studies, the purified enzyme has great potential to be developed as a natural agent for prevention and treatment of thrombolytic diseases. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Dose dependency of outcomes of intrapleural fibrinolytic therapy in new rabbit empyema models.

    Science.gov (United States)

    Komissarov, Andrey A; Florova, Galina; Azghani, Ali O; Buchanan, Ann; Boren, Jake; Allen, Timothy; Rahman, Najib M; Koenig, Kathleen; Chamiso, Mignote; Karandashova, Sophia; Henry, James; Idell, Steven

    2016-08-01

    The incidence of empyema (EMP) is increasing worldwide; EMP generally occurs with pleural loculation and impaired drainage is often treated with intrapleural fibrinolytic therapy (IPFT) or surgery. A number of IPFT options are used clinically with empiric dosing and variable outcomes in adults. To evaluate mechanisms governing intrapleural fibrinolysis and disease outcomes, models of Pasteurella multocida and Streptococcus pneumoniae were generated in rabbits and the animals were treated with either human tissue (tPA) plasminogen activator or prourokinase (scuPA). Rabbit EMP was characterized by the development of pleural adhesions detectable by chest ultrasonography and fibrinous coating of the pleura. Similar to human EMP, rabbits with EMP accumulated sizable, 20- to 40-ml fibrinopurulent pleural effusions associated with extensive intrapleural organization, significantly increased pleural thickness, suppression of fibrinolytic and plasminogen-activating activities, and accumulation of high levels of plasminogen activator inhibitor 1, plasminogen, and extracellular DNA. IPFT with tPA (0.145 mg/kg) or scuPA (0.5 mg/kg) was ineffective in rabbit EMP (n = 9 and 3 for P. multocida and S. pneumoniae, respectively); 2 mg/kg tPA or scuPA IPFT (n = 5) effectively cleared S. pneumoniae-induced EMP collections in 24 h with no bleeding observed. Although intrapleural fibrinolytic activity for up to 40 min after IPFT was similar for effective and ineffective doses of fibrinolysin, it was lower for tPA than for scuPA treatments. These results demonstrate similarities between rabbit and human EMP, the importance of pleural fluid PAI-1 activity, and levels of plasminogen in the regulation of intrapleural fibrinolysis and illustrate the dose dependency of IPFT outcomes in EMP.

  14. [The fibrinolytic treatment with urokinase of acute arterial thrombosis].

    Science.gov (United States)

    Ballester, A; Donato di Paola, M; Saccà, A; Cappello, I; D'Addato, M

    1993-01-01

    We present our experiences on 86 patients with acute arterial thrombosis of the legs, undergoing a fibrinolytic treatment with urokinase. Results from the treatment are analyzed according to: the administration way (systemic, locoregional, intrathrombotic), the level of thrombosis (upper or lower legs), the associated morbidity and mortality.

  15. Compared with the Dissolubility Proteins and Activity of Fibrinolytic Enzyme between Fresh Earthworm and Dry Earthworm%地龙的鲜品和干品可溶性蛋白及纤溶酶活性的对比研究

    Institute of Scientific and Technical Information of China (English)

    陈丽艳; 张迎; 綦菲; 宋芳婷; 王伟明

    2012-01-01

    目的:对比研究地龙的鲜品和干品的可溶性蛋白及纤溶酶活性.方法:采用Bradfold方法测定蛋白含量,SDS-PAGE进行蛋白谱带的分析,并采用琼脂糖-纤维蛋白平板法进行纤溶酶活性的测定.结果:以干燥品计,鲜品的蛋白提取率为( 13.89±0.86)%,蛋白含量为(63.14±0.38)%,体外纤溶酶活性为(8 743 ±17) IU·g-1;干品蛋白提取率为(9.82±0.75)%,蛋白含量为(45.16 ±0.12)%,体外纤溶酶活性为(206±14) IU·g-1;SDS-PAGE凝胶电泳结果显示,鲜品共显示12条蛋白谱带,干品的谱带较少,且不清晰.结论:鲜地龙的可溶性蛋白提取率、含量及体外纤溶酶活性均明显高于干品,且蛋白组分有较大的差异.%Objective; To study the dissolubility proteins and activity of fibrinolytic enzyme of fresh and dry earthworm comparably. Method: Bradfold method was used to detect protein content, SDS-PAGE was used to analyze the protein compositions, and the agarose-fibrin plate was used to detect the activity of fibrinolytic enzyme. Result; On dried basis, the protein extraction rate of fresh earthworm was (13. 89 ±0. 86)% , the protein content was (63. 14 ± 0. 38) % , the activity of enzyme led to (8 743 ± 17 ) LU · kg' ; compared with dry earthworm, the protein extraction rate was (9. 82 ±0. 75)% , the protein content and enzyme activity were (45. 16 ±0. 12)% and (206 ±14) IU ·kg'. The SDS-PAGE result showed that there were 12 protein bands in fresh earthworm and only several bands in dry earthworm. Conclusion: The extraction rate, content and enzyme activity of the dissolubility proteins at fresh earthworm were higher than dry earthworm, and the constituents exhibited obvious difference.

  16. 贵州豆豉纤溶酶发酵液中活性酶的盐析分离纯化%Separation and Purification of Active Enzyme from Fibrinolytic Enzyme Fermentation Broth of Guizhou Lobster Sauce with Salting Fractionation Method

    Institute of Scientific and Technical Information of China (English)

    张敏; 宋智魁; 王鹏娇; 王清忱; 彭安堂; 舒阳; 高秀丽

    2013-01-01

    Objective:To culture fibrinolytic enzyme strains (DCK-B)extracted from Guizhou characteristic lobster sauce with liquid ferment method,and to develop a method for primary purification of active fibrinolytic enzyme from the fermentation products.Methods:Ammonium sulphate (AS)salting out method was used for primary purification,and the effects of one step salting out method with 0% ~ 80% AS and two step salting out method with AS of 0% ~ 30% and 30% ~ 60% were compared.Results:The precipitate was impure protein when AS concentration was lower than 30%.A lot of target protein precipitated when AS concentration was higher than 30%.When the concentration was up to 50%,almost all the active protein precipitated.It indicated that a few active protein was salted out when AS concentration was between 0 ~ 30% and most of active protein salted out when AS concentration was between 30% ~ 60%.Conclusions:The two step AS salting out method for separation and purification of active enzyme from fibrinolytic enzyme fermentation broth of Guizhou lobster sauce is established.%目的:对贵州豆豉发酵液中纤溶酶分离纯化.方法:贵州特色药食两用的豆豉中提取得到的纤溶酶菌株(DCK-B),用液体发酵法将其发酵,采用硫酸铵盐析法进行粗提纯,并对0~80%浓度的一级硫酸铵梯度盐析与0~30%、30%~ 60%两段硫酸铵梯度盐析的结果进行比较.结果:硫酸铵梯度盐析在30%以前出现的沉淀为杂蛋白,且较复杂;30%梯度以后的目标蛋白大量沉淀出来,到50%时活性蛋白几乎沉淀完全;经两段盐析,当0~30%盐析时有少量活性蛋白盐析出来,绝大部分蛋白在30%~ 60%这个盐析梯度沉降下来.结论:确定了贵州豆豉纤溶酶发酵液中活性酶的两段硫酸铵盐析分离纯化方法.

  17. Anti-inflammatory and anti-fibrinolytic effects of thrombomodulin alfa through carboxypeptidase B2 in the presence of thrombin.

    Science.gov (United States)

    Tawara, Shunsuke; Sakai, Takumi; Matsuzaki, Osamu

    2016-11-01

    Thrombomodulin (TM) alfa, a recombinant human soluble TM, enhances activation of pro-carboxypeptidase B2 (pro-CPB2) by thrombin. Activated pro-CPB2 (CPB2) exerts anti-inflammatory and anti-fibrinolytic activities. Therefore, TM alfa may also have anti-inflammatory and anti-fibrinolytic effects through CPB2. However, these effects of TM alfa have not been elucidated. In the present study, we investigated the effects of TM alfa on inactivation of complement component C5a as an anti-inflammatory effect and prolongation of clot lysis time as an anti-fibrinolytic effect via CPB2 in vitro. CPB2 activity and tissue factor-induced thrombin generation was examined by a chromogenic assay. C5a inactivation was evaluated by C-terminal cleavage of C5a and inhibition of C5a-induced human neutrophil migration. Clot lysis time prolongation was examined by a tissue-type plasminogen activator-induced clot lysis assay. CPB2 activity in human plasma was increased by TM alfa and thrombin in a concentration-dependent manner. TM alfa inhibited tissue factor-induced thrombin generation and enhanced pro-CPB2 activation in human plasma simultaneously. The mass spectrum of C5a treated with TM alfa, thrombin, and pro-CPB2 was decreased at 156m/z, indicating that TM alfa enhanced the processing of C5a to C-terminal-cleaved C5a, an inactive form of C5a. C5a-induced human neutrophil migration was decreased after C5a treatment with TM alfa, thrombin, and pro-CPB2. TM alfa prolonged the clot lysis time in human plasma, and this effect was completely abolished by addition of a CPB2 inhibitor. TM alfa exerts anti-inflammatory and anti-fibrinolytic effects through CPB2 in the presence of thrombin in vitro. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Long-term stability of fibrinolytic factors stored at -80 degrees C.

    Science.gov (United States)

    Hernestål-Boman, Jenny; Jansson, Jan-Håkan; Nilsson, Torbjörn K; Eliasson, Mats; Johansson, Lars

    2010-05-01

    Blood samples in epidemiological studies are often stored for several years and analysed at different occasions. The reagent kits are continually modified for better precision and accuracy. Our hypothesis was that epidemiological studies are affected by long-term storage and/or modifications of reagent kits. Plasma samples stored at -80( degrees )C from two populations were used: A case-referent study with samples collected from 1985 to 2000 and analysed 2005 (n=1598) were used to study influence of long-term storage. A cross-sectional study analysed 1990 (n=1558) and re-analysed 2001 (n=78) and 2005 (n=828) was used to study influence of reagent kit modifications. Fibrinolytic analyses included immunoassays of tPA, PAI-1 and tPA-PAI-1 complex and chromogenic substrate assays of the activities of tPA and PAI-1. Long-term storage for a median time of 11.6 years (range 5 to 20) showed an effect of time on tPA antigen R(2)=0.01, PAI-1 antigen R(2)=0.01 and tPA-PAI-1 complex R(2) = 0.02. Modifications in reagent kits affected the levels of fibrinolytic factors; for tPA antigen the slope coefficients were between 0.72 and 0.95 (R(2) 0.47 - 0.75), whereas tPA activity showed an agreement with slope coefficients 1.06 to 1.09 (R(2) 0.67 - 0.93). This study showed that long-term storage affects fibrinolytic variables to a negligible extent, but modifications in reagent kits introduced an element of bias. We conclude that analysis of samples on a single occasion is preferable to multiple occasions, as storage has negligible effect. Copyright (c) 2009 Elsevier Ltd. All rights reserved.

  19. Purification and characterization of a novel fibrinolytic enzyme from culture supernatant of Pleurotus ostreatus.

    Science.gov (United States)

    Liu, Xiao-Lan; Zheng, Xi-Qun; Qian, Peng-Zhi; Kopparapu, Narasimha-Kumar; Deng, Yong-Ping; Nonaka, Masanori; Harada, Naoki

    2014-02-28

    A fibrinolytic enzyme was produced by an edible mushroom of Pleurotus ostreatus using submerged culture fermentation. The enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. The enzyme was purified by a 147-fold, with a yield of 7.54%. The molecular masses of the enzyme an determined by gel filtration and SDSPAGE were 13.6 and 18.2 kDa, respectively. The isoelectric point of the enzyme was 8.52. It hydrolyzed fibrinogen by cleaving the α and β chains of fibrinogen followed by the γ chains, and also activated plasminogen into plasmin. The enzyme was optimally active at 45°C and pH 7.4. The enzyme activity was completely inhibited by EDTA, whereas protease inhibitors of TPCK, SBTI, PMSF, aprotinin and pepstatin showed no inhibition on its activity. The partial amino acid sequences of the enzyme as determined by Q-TOF2 were ATFVGCSATR, GGTLIHESSHFTR, and YTTWFGTFVTSR. These sequences showed a high degree of homology with those of metallo-endopeptidases from P. ostreatus and Armillaria mellea. The purified enzyme can also be applied as a natural agent for oral fibrinolytic therapy or prevention of thrombosis.

  20. Verticase:a Fibrinolytic Enzyme Produced by Verticillium sp.Tj33,an Endophyte of Trachelospermum jasminoides

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Plant endophytes are among the most important resources of biologically active metabolites.Twenty-three endophyte strains residing in Trachelospermum jasminoides were cultivated In with the cultures assayed for the fibrinolytic substance production.As a result,the culture of Verticillium sp.Tj33 was shown to be the most active.A fibrinolytic enzyme designated as verticase was subsequently purified from the supernatant of Verticillium sp.culture broth by a combination of DEAE-52,Sephadex G-75 and hydrophobic column chromatographies.Verticase,with its molecular mass of 31 kDa and pl of 8.5,was demonstrated to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing electrophoresis.Verticase is an enzyme that hydrolyzes fibrin directly without activation of plaminogen.It was stable in a broad pH range from 4 through to 11 with the optimal reaction pH value and temperature shown to be around 9-10 and 50-60℃,respectively.The fibrinolytic activity of verticase was severely inhibited by phenylmethylsulfony fluoride,Indicating that verticase was a serine protease.

  1. Effects of whole-body vibration training on fibrinolytic and coagulative factors in healthy young men

    Directory of Open Access Journals (Sweden)

    Farshad Ghazalian

    2014-01-01

    Full Text Available Background: The aim was to evaluate effects of 5-week whole body vibration (WBV training with different amplitudes and progressive frequencies on fibrinolytic/coagulative factors. Materials and Methods: 25 subjects were divided randomly in high or low-amplitude vibration, and control groups. Training consisted of 5-week WBV with amplitudes 4 or 2 mm. Plasma samples were analyzed before and after training. Statistical analysis was done using one-way analysis of variance and Wilcoxon signed ranked test. P <0.05 was considered significant. Results: High-amplitude vibration caused an increase in tissue plasminogen activator (tPA (P = 0.028 (pretest: 1744.61 ± 707.95; posttest: 2313.63 ± 997.19 pg/ml, and decrease in plasminogen activator inhibitor-1 (PAI-1 (P = 0.033 (pretest: 97.94 ± 34.37; posttest: 85.12 ± 36.92 ng/ml. Fibrinogen and plasminogen were not changed significantly. Low-amplitude vibration caused an increase in tPA (P = 0.006 (pretest: 2208.18 ± 1280.37; posttest: 3492.72 ± 3549.22 pg/ml. PAI-1, fibrinogen and plasminogen were not changed significantly. There were no significant differences between groups. Conclusion: Amplitude of vibrations in WBV training may affect fibrinolytic factors.

  2. [Besides fibrinogen, are haemostatic, coagulation and/or fibrinolytic parameters predictors or markers of the risk of cardiovascular events?].

    Science.gov (United States)

    Drouet, Ludovic; Bal dit Sollier, Claire

    2005-01-01

    Most cardiovascular events result from a thrombotic complication of atherosclerotic lesions. In arterial vessels such as the coronary bed, an interrelationship of haemostatic, coagulation and fibrinolytic factors is implicated. While it can be demonstrated that fibrinogen is a risk factor/marker, the role of other factors is not well established. Under arterial flow conditions, platelets are predominantly involved in the thrombotic reaction. Yet, apart from a large increase in the platelet count, the involvement of platelet parameters in cardiovascular risk is not clearly evident. The lack of definitive platelet markers is at least partly due to the difficulty of studying platelet function ex vivo. Several polymorphisms of platelet glycoproteins carrying a moderate increase in risk have been reported, but only in younger patients. One potentially important factor for coagulation is the fibrin structure, which is dependent on fibrinogen, the rate of thrombin generation, the activity of factor XIII and the interrelationship of the cells concerned, all of which act on its sensitivity to thrombosis. Coagulation factors largely affect the rate of thrombin generation. The activity of the fibrinolytic system (and principally any deficiency) has a role in the cardiovascular risk. General markers of cardiovascular risk such as D-dimers are potentially useful, but they increase with thrombin generation and are decreased by a deficiency in fibrinolysis. Furthermore, possibly because they are not indicative of the fibrin structure, they are poorly correlated with clinical events. The poor significance of the available haemostatic, coagulation and/or fibrinolytic parameters is probably due to their lack of representativeness, since haemostatic, coagulation and fibrinolytic systems are all involved in the thrombotic response (and some in atherogenesis itself). Atherogenesis is a multifactorial process and numerous moderate risk factors act in association. Better predictability

  3. Production, Purification and Characterisation of a Potential Fibrinolytic Protease from Endophytic Xylaria curta by Solid Substrate Fermentation.

    Science.gov (United States)

    Meshram, Vineet; Saxena, Sanjai; Paul, Karan; Gupta, Mahiti; Kapoor, Neha

    2017-04-01

    The present investigation highlights the optimal conditions for production of a non-toxic, bi-functional fibrinolytic enzyme xylarinase produced by endophytic fungus Xylaria curta by solid substrate fermentation using rice chaff medium. The purified enzyme is a monomeric protein with a molecular mass of ∼33 kDa. The enzyme exhibits cleavage of Aα and Bβ chains of fibrin(ogen) and has no effect on γ chain. The optimal fibrinolytic activity of the enzyme was observed at 35 °C and pH 8. The fibrinolytic activity was enhanced in the presence of Ca(2+), whereas it was completely inhibited in the presence of Fe(2+) and Zn(2+) ions and inhibitors like EDTA and EGTA suggesting it to be a metalloprotease. The K m and V max of the enzyme for azocasein were 326 μM and 0.13 μM min(-1). The N-terminal sequence of the enzyme (SNGPLPGGVVWAG) was same when compared to xylarinase isolated from culture broth of X. curta. Thus, xylarinase could be exploited as a potent clot busting enzyme which could be produced on large scale using solid substrate fermentation.

  4. Production of fibrinolytic enzyme from Bacillus amyloliquefaciens by fermentation of chickpeas, with the evaluation of the anticoagulant and antioxidant properties of chickpeas.

    Science.gov (United States)

    Wei, Xuetuan; Luo, Mingfang; Xu, Lin; Zhang, Yewei; Lin, Xing; Kong, Peng; Liu, Huizhou

    2011-04-27

    To develop safe and cheap thrombolytic agents, a fibrinolytic enzyme productive strain of LSSE-62 was isolated from Chinese soybean paste. This strain was identified as Bacillus amyloliquefaciens by 16S rDNA sequence analysis. Nucleotide and amino acid sequence analysis showed that this fibrinolytic enzyme was identical to subtilisin DJ-4. Chickpeas were used as the substrate for fibrinolytic enzyme production from B. amyloliquefaciens in solid-state fermentation. Under the optimized conditions (34 °C and 50% initial moisture content), the fibrinolytic activity of fermented chickpeas reached 39.28 fibrin degradation units (FU)/g. Additionally, the fermented chickpeas showed anticoagulant activity, and the purified anticoagulant component showed higher anticoagulant activity than heparin sodium. After fermentation, the total phenolic and total flavonoid contents increased by 222 and 71%, respectively, and then the antioxidant activities were improved significantly. This study provided a novel method for the preparation of multifunctional food of chickpeas or raw materials for the preparation of functional food additives and potential drugs.

  5. Multi-isomorphous replacement phasing of the earthworm fibrinolytic enzyme component A from Eisenia fetida

    Institute of Scientific and Technical Information of China (English)

    汤涌; 江涛; 张季平; 樊蓉; 吴骋; 梁栋材; 常文瑞

    2003-01-01

    Earthworm fibrinolytic enzyme component A (EFEa) from Eisenia fetida, a protein functioning not only as a direct fibrinolytic enzyme, but also as a plasminogen activator, has been crystallized in P212121 space group with 3 proteinmolecules per asymmetric unit. Four heavy atom derivatives were prepared using a mother liquor containing 1.4 mol@L-1 Li2SO4 and 0.1 mol@L-1 MOPS buffer (pH7.2) and used to solve the protein's diffraction phase. The heavy atom binding sites in the derivative crystals were determined using difference Patterson and difference Fourier methods and were refined in combination to yield the initial protein's structure phase at 0.25 nm resolution. The non-crystallographic symmetryrelationship of the three independent protein molecules in the asymmetric unit was determined using the correlative heavy atom sites and used for the averagingof the initial electron density. As a result, the electron density was significantly improved, providing a solid foundation for subsequent structure determination.

  6. Optimization of fibrinolytic enzyme production by Bacillus subtilis DC-2 in aqueous two-phase system (poly-ethylene glycol 4000 and sodium sulfate).

    Science.gov (United States)

    Ashipala, Onesmus K; He, Qian

    2008-07-01

    The central composite rotable design (CCRD) was used to determine optimal conditions for fibrinolytic enzyme production by Bacillus subtilis DC-2 in poly-ethylene glycol 4000 (PEG 4000) and sodium sulfate (Na(2)SO(4)) aqueous two-phase system (ATPS). PEG 4000 and Na(2)SO(4) concentration, fermentation time and temperature, and pH were selected as variables to evaluate the fibrinolytic activity in PEG phase. Using response surface methodology (RSM), a second-order polynomial equation was obtained by multiple regression analysis. The predicted maximal fibrinolytic activity in PEG phase was 1241.02 IU/ml with 9.05% PEG 4000 concentration, 5.06% Na(2)SO(4) concentration, 118.77 h fermentation time, 37.57 degrees C fermentation temperature and pH 6.52. The validity of the response model was verified by a good agreement between predicted and experimental results. The fibrinolytic activity obtained from experimental results in PEG phase (1223.61 IU/ml) was higher than that produced in homogeneous fermentation (1165.58 IU/ml).

  7. FA

    African Journals Online (AJOL)

    spamer

    Portugal, Senegal and Ivory Coast-. Ghana have ..... sometimes much higher than that during the last century in California or in ... assessments of past and present experiences can be ... Some scenarios for fish productivity under climatic changes ...

  8. Adrenergic, coagulation, and fibrinolytic responses to heat.

    Science.gov (United States)

    Britton, B J; Hawkey, C; Wood, W G; Peele, M; Kaye, J; Irving, M H

    1974-10-19

    Two groups of volunteers were exposed to heat in a sauna bath-one group for 10 minutes and the other for 15. There was no change in plasma adrenaline concentration until the subjects emerged from the sauna bath, when there was a slight increase in concentration. Factor VIII and thrombo-elastograph patterns did not change but marked activation of fibrinolysis was stimulated by exposure to heat. These findings support the concept that fibrinolysis is not mediated by direct adrenergic activity.

  9. Spontaneous hemarthrosis following fibrinolytic therapy for acute myocardial infarction: a case report and literature review.

    Science.gov (United States)

    Ramadan, Mahmoud M; Khan, Iqbal S; Mahdi, Ousama

    2014-11-23

    Background Despite the widespread use of fibrinolytic therapy and the numerous reports on its bleeding complications, spontaneous hemarthrosis following fibrinolytic therapy is quite rare. Case Report We describe in this report a patient with no previous history of articular disease who developed a spontaneous right knee bloody effusion following fibrinolytic therapy using rt-PA for acute ST-elevation myocardial infarction. Furthermore, we provide a review of all cases of spontaneous hemarthrosis documented so far in the literature. Conclusions Several pre-existing joint diseases may predispose to hemarthrosis following fibrinolytic therapy, even in patients who deny previous or current articular disorders. Therefore, hemorrhage should be considered in the differential diagnosis of mono-arthritis following fibrinolytic therapy for STEMI.

  10. Zaznamek k fašistoidnim praksam in vzpostavljanju novega fašizma

    Directory of Open Access Journals (Sweden)

    Jernej Prodnik

    2013-03-01

    Full Text Available Avtor podaja teoretsko-analitičen premislek h konceptualizaciji fašizma, ki ga v zadnjem delu besedila aplicira na aktualne politično-ekonomske in politične razmere. Za razumevanje fašizma je treba tega izločiti iz zgodovinskega konteksta, čemur mora pri praktični analizi slediti vsakokratna in sočasna ponovna umestitev v aktualne zgodovinske procese v družbi. Avtor predlaga ločevanje med izgrajenim fašističnim redom in fašistoidnimi institucijami, procesi, praksami in diskurzi, ki nakazujejo diferenciacijo med makroravnjo in mikropraksami ter politikami na ravni vsakdanjega življenja. Primeren družbeni kontekst je namreč predpogoj za gradnjo celovitejšega reda, saj daje podlago za premestitev oziroma prenos teh praks na širšo družbeno raven (na primer v razširjen skupek fašistoidnih aparatov in institucij na nacionalni ali nadnacionalni ravni, ki potencialno omogočajo fašistični red. S tem je redefinirana trdna in ostra binarna opozicija med fašističnimi in nefašističnimi družbami, saj v različnih zgodovinskih trenutkih obstaja možnost gibanja od navidezno benignih fašistoidnih praks do protofašističnega konteksta in končno do celovito zgrajenega fašizma. Na ta način pa je mogoče fašizem opazovati in analizirati tudi kot gibanje in proces, ne le kot (zgrajeno strukturo. /// English title: A Note on Fascist Practices (and the Emergence of Neo-Fascism // Abstract in English: The author provides a theoretical analysis of the concept of fascism, which he then applies to the present political-economic and political circumstances. He proposes that to understand fascism it is important to separate it from the historical context, while it is at the same time of crucial importance to embed it into the current historical processes in the society. Only this kind of abstraction will enable us to consider fascism in a patently changed, but in many ways similar social context. Furthermore a differentiation is

  11. Fibrinolytic markers and neurologic outcome in traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Bayir Aysegul

    2006-01-01

    Full Text Available Aims: To determine the usefulness of fibrinolytic markers as early prognostic indicators in patients with isolated head trauma. Materials and Methods: Sixty-two consecutive patients (26 women and 36 men; mean age 61 years, range 2-76 years with isolated head trauma seen within the first three hours of the trauma were included in the study. The Glasgow Coma score (GCS, platelet counts (Plt, prothrombin time (PT, partial thromboplastin time (PTT, fibrinogen, fibrin degradation products (FDP and D-dimer levels were measured. Head computerized tomography (CT findings were categorized as brain edema, linear fracture, depressed fracture, contusion and bleeding. Plt counts, PT, PTT, fibrinogen, FDP, D-dimer levels and CT findings were compared with both GCS and mortality in the first week. Statistical significance was accepted at P£0.05. Results: A marked negative relationship was found between GCS and PT, PTT, FDP and D-dimer levels ( P < 0.001. Plt levels did not correlate with GCS. Mortality was most strongly related to GCS, PT, FDP and D-dimer levels ( P < 0.001, P < 0.001, P < 0.001 and P < 0.001, respectively. We found no relationship between mortality and CT findings, nor was there any significant relationship between Plt, PTT and fibrinogen levels. Conclusion: GCS and fibrinolytic markers measured within the first three hours were useful in determining the prognosis of patients with isolated head trauma.

  12. Purification and biochemical characterization of a novel fibrinolytic enzyme, PSLTro01, from a medicinal animal Porcellio scaber Latreille.

    Science.gov (United States)

    Tian, Zhou; Li, Bo; Guo, Liwei; Wu, Mianhua; Fu, Tingming; Cheng, Haibo; Zhu, Huaxu

    2015-09-01

    A novel protease, named PSLTro01, with fibrinolytic and anticoagulant activity was isolated from Porcellio scaber Latreille and was purified by a combination of hollow fibre membrane molecular weight cut-off (MWCO), ammonium sulfate fractionation, gel filtration and ion-exchange chromatography. PSLTro01 is a single-chain protein with a molecular mass of 38,497 Da as estimated by non-reduced SDS-PAGE and MALDI-TOF MS spectrometry, and its N-terminal 15 amino acid sequence was determined as DINGGGATLPQPLYQ. PSLTro01 is stable in the range of 20-40 °C and pH 6.0-10.0, with a maximum fibrinolytic activity at 40 °C and pH 7.0. The PSLTro01-induced fibrinolytic activity was not influenced by K(+) or Na(+) but was slightly increased by Mg(2+) and completely inhibited by aprotinin and pepstatin A. Fibrin plate assays revealed that PSLTro01 could not directly degrade fibrin but was a plasminogen activator. PSLTro01 exhibited high specificity for the substrate S-2251 for plasmin, followed by S-2238 for thrombin and S-2444 for urokinase. Moreover, the fibrinogenolysis pattern of PSLTro01 was Aα-chains>Bβ-chains>γ-chain. Tail-thrombus of the enzyme treated group was significantly shorter than the physiological saline treated group and the thrombus decrement was correlated with the enzyme dose. PSLTro01 prolongs both thrombin time (TT) and activated partial thromboplastin time (APTT). These results indicate that PSLTro01 may have potential applications in the prevention and treatment of thrombosis.

  13. Influence of dietary saturated fat intake on endothelial fibrinolytic capacity in adults.

    Science.gov (United States)

    Dow, Caitlin A; Stauffer, Brian L; Greiner, Jared J; DeSouza, Christopher A

    2014-09-01

    Approximately 50% of middle-aged and older adults in the United States regularly consume a diet high in saturated fat. High dietary saturated fat intake has been linked to promote atherothrombotic vascular disease. We tested the hypothesis that endothelial fibrinolytic function is diminished in middle-aged and older adults who habitually consume a diet high in saturated fat. Twenty-four healthy, sedentary middle-aged, and older adults (54 to 71 years) were studied: 10 (8 men and 2 women) with a dietary saturated fat intake saturated fat) of total calories and 14 (9 men and 5 women) with a dietary saturated fat intake ≥10% of total calories (high saturated fat). Net endothelial release of tissue-type plasminogen activator (t-PA), the primary activator of fibrinolysis, was determined, in vivo, in response to intrabrachial infusions of bradykinin (12.5 to 50.0 ng/100 ml tissue/min) and sodium nitroprusside (1.0 to 4.0 μg/100 ml tissue/min). Capacity of the endothelium to release t-PA in response to bradykinin was ∼30% less (p saturated fat group. Moreover, total amount of t-PA released was significantly less (∼30%) (201 ± 22 vs 274 ± 29 ng/100 ml tissue) in the adults who reported consuming a diet high in saturated fat. These results indicate that the capacity of the endothelium to release t-PA is lower in middle-aged and older adults who habitually consume a diet high in saturated fat. In conclusion, endothelial fibrinolytic dysfunction may underlie the increased atherothrombotic disease risk with a diet high in saturated fat.

  14. Physiological Effect of New FA Antitranspirant Application on Maize

    Institute of Scientific and Technical Information of China (English)

    LI Mao-song; LI Sen; ZHANG Shu-yi; CHI Bao-liang

    2003-01-01

    The field trial was conducted to study physiological effect of new FA antitranspirant on maize.The new FA antitranspirant was sprayed at 10 d pre-tasseling, ear filling stage and 10 d pre-tasseling + earfilling stage, with the concentrations of 0, 0.5, 1.0, 1.5 and 2.0 ml L-1. The results indicated that the appli-cation of new FA antitranspirant increased nitrate reductase activity (NRA), free proline content, chlorophyllcontent and water content of leaf, thus drought stress can be mitigated. The new FA antitranspirant increasedphotosynthesis rate and reduced transpiration rate, stimulated growth and reduced water loss. 10 d pre-tassel-ing + ear filling stage application had a cumulative effect on the indices compared with 10 d pre-tasseling andear filling stage, except for NRA. The new FA antitranspirant caused an increase of grain yield by 5.37 to29.58 % with different treatments. The optimal concentration is 1.5 ml L-1 , i.e. 75 g new FA antitranspirantdissolved in 50 kg water, and the optimal apply period is 10 d pre-tasseling ± ear filling stage.

  15. FaPOD27 functions in the metabolism of polyphenols in strawberry fruit (Fragaria sp.).

    Science.gov (United States)

    Yeh, Su-Ying; Huang, Fong-Chin; Hoffmann, Thomas; Mayershofer, Mechthild; Schwab, Wilfried

    2014-01-01

    The strawberry (Fragaria × ananassa) is one of the most preferred fresh fruit worldwide, accumulates numerous flavonoids but has limited shelf life due to excessive tissue softening caused by cell wall degradation. Since lignin is one of the polymers that strengthen plant cell walls and might contribute to some extent to fruit firmness monolignol biosynthesis was studied in strawberry fruit. Cinnamoyl-CoA reductase (CCR), cinnamyl alcohol dehydrogenase (CAD), and a peroxidase (POD27) gene were strongly expressed in red, ripe fruit whereas a second POD gene was primarily expressed in green, immature fruit. Moreover, FaPOD27 transcripts were strongly and constitutively induced in fruits exposed to Agrobacterium infection. Gene expression levels and enzymatic activities of FaCCR and FaCAD were efficiently suppressed through RNAi in FaCCR- and FaCAD-silenced strawberries. Besides, significantly elevated FaPOD transcript levels were detected after agroinfiltration of pBI-FaPOD constructs in fruits. At the same time, levels of G-monomers were considerably reduced in FaCCR-silenced fruits whereas the proportion of both G- and S-monomers decisively decreased in FaCAD-silenced and pBI-FaPOD fruits. Development, firmness, and lignin level of the treated fruits were similar to pBI-intron control fruits, presumably attributed to increased expression levels of FaPOD27 upon agroinfiltration. Additionally, enhanced firmness, accompanied with elevated lignin levels, was revealed in chalcone synthase-deficient fruits (CHS(-)), independent of down- or up-regulation of individual and combined FaCCR. FaCAD, and FaPOD genes by agroinfiltration, when compared to CHS(-)/pBI-intron control fruits. These approaches provide further insight into the genetic control of flavonoid and lignin synthesis in strawberries. The results suggest that FaPOD27 is a key gene for lignin biosynthesis in strawberry fruit and thus to improving the firmness of strawberries.

  16. FaPOD27 functions in the metabolism of polyphenols in strawberry fruit (Fragaria sp.

    Directory of Open Access Journals (Sweden)

    Su-Ying eYeh

    2014-10-01

    Full Text Available The strawberry (Fragaria × ananassa is one of the most preferred fresh fruit worldwide, accumulates numerous flavonoids but has limited shelf life due to excessive tissue softening caused by cell wall degradation. Since lignin is one of the polymers that strengthen plant cell walls and might contribute to some extent to fruit firmness monolignol biosynthesis was studied in strawberry fruit. Cinnamoyl-CoA reductase (CCR, cinnamyl alcohol dehydrogenase (CAD, and a peroxidase (POD27 gene were strongly expressed in red, ripe fruit whereas a second POD gene was primarily expressed in green, immature fruit. Moreover, FaPOD27 transcripts were strongly and constitutively induced in fruits exposed to Agrobacterium infection. Gene expression levels and enzymatic activities of FaCCR and FaCAD were efficiently suppressed through RNAi in FaCCR- and FaCAD-silenced strawberries. Besides, significantly elevated FaPOD transcript levels were detected after agroinfiltration of pBI-FaPOD constructs in fruits. At the same time, levels of G-monomers were considerably reduced in FaCCR-silenced fruits whereas the proportion of both G- and S-monomers decisively decreased in FaCAD-silenced and pBI-FaPOD fruits. Development, firmness, and lignin level of the treated fruits were similar to pBI-intron control fruits, presumably attributed to increased expression levels of FaPOD27 upon agroinfiltration. Additionally, enhanced firmness, accompanied with elevated lignin levels, was revealed in chalcone synthase-deficient fruits (CHS-, independent of down- or up-regulation of individual and combined FaCCR, FaCAD, and FaPOD genes by agroinfiltration, when compared to CHS-/pBI-intron control fruits. These approaches provide further insight into the genetic control of flavonoid and lignin synthesis in strawberries. The results suggest that FaPOD27 is a key gene for lignin biosynthesis in strawberry fruit and thus to improving the firmness of strawberries.

  17. The diversity and extracellular enzymatic activities of yeasts isolated from water tanks of Vriesea minarum, an endangered bromeliad species in Brazil, and the description of Occultifur brasiliensis f.a., sp. nov.

    Science.gov (United States)

    Gomes, Fátima C O; Safar, Silvana V B; Marques, Andrea R; Medeiros, Adriana O; Santos, Ana Raquel O; Carvalho, Cláudia; Lachance, Marc-André; Sampaio, José Paulo; Rosa, Carlos A

    2015-02-01

    The diversity of yeast species collected from the bromeliad tanks of Vriesea minarum, an endangered bromeliad species, and their ability to produce extracellular enzymes were studied. Water samples were collected from 30 tanks of bromeliads living in a rupestrian field site located at Serrada Piedade, Minas Gerais state, Brazil, during both the dry and rainy seasons. Thirty-six species were isolated, representing 22 basidiomycetous and 14 ascomycetous species. Occultifur sp., Cryptococcus podzolicus and Cryptococcus sp. 1 were the prevalent basidiomycetous species. The yeast-like fungus from the order Myriangiales, Candida silvae and Aureobasidium pullulans were the most frequent ascomycetous species. The diversity of the yeast communities obtained between seasons was not significantly different, but the yeast composition per bromeliad was different between seasons. These results suggest that there is significant spatial heterogeneity in the composition of populations of the yeast communities within bromeliad tanks, independent of the season. Among the 352 yeast isolates tested, 282 showed at least one enzymatic activity. Protease activity was the most widely expressed extracellular enzymatic activity, followed by xylanase, amylase, pectinase and cellulase activities. These enzymes may increase the carbon and nitrogen availability for the microbial food web in the bromeliad tank of V. minarum. Sequence analyses revealed the existence of 10 new species, indicating that bromeliad tanks are important sources of new yeasts. The novel species Occultifur brasiliensis, f.a., sp. nov., is proposed to accommodate the most frequently isolated yeast associated with V. minarum. The type strain of O. brasiliensis, f.a., sp. nov. is UFMG-CM-Y375(T) (= CBS 12687(T)). The Mycobank number is MB 809816.

  18. Positive impact of hormone replacement therapy on the fibrinolytic system: a long-term randomized controlled study in healthy postmenopausal women

    DEFF Research Database (Denmark)

    Madsen, J S; Kristensen, S R; Bladbjerg, E M;

    2003-01-01

    be of importance. OBJECTIVES: To investigate the prolonged effect of HRT on the fibrinolytic system and to determine whether two common polymorphisms in the plasminogen activator inhibitor-1 (PAI-1) and tissue-type plasminogen activator (t-PA) genes modulate this effect. Methods: Healthy postmenopausal women (n......BACKGROUND: The mechanisms by which postmenopausal hormone replacement therapy (HRT) may influence risk of cardiovascular disease are still unclear. Impaired fibrinolytic function is associated with an enhanced risk of cardiovascular disease and therefore the effect of HRT on fibrinolysis may...... of smoking and without influence from the two common polymorphisms PAI-1 -675(4G/5G) and t-PA intron8ins311. Furthermore, no difference between opposed estrogen (with norethisterone acetate as the gestagen component) and unopposed estrogen therapy was found. Both an intention-to-treat and a per...

  19. Impact of fibrinolytics on the outcome of empyema in South African ...

    African Journals Online (AJOL)

    introduction of 7-valent pneumococcal conjugate vaccine (PCV7), the incidence of ... after the child received fibrinolytics were included in the treatment analysis. ... pleural effusion causing symptoms (pain ... Persistent fever >38°C more than.

  20. Ultrasound-enhanced Fibrinolysis Pro-fibrinolytic and Non-beneficial Effects Of Ultrasound Exposure

    OpenAIRE

    Madsen-Härdig, Bjarne

    2005-01-01

    The aim of this study was to further clarify the pro-fibrinolytic effects, and to explore the possible non-beneficial effects in ischemic organs, during exposure to pulsed ultrasound. This was accomplished by studies of the effects of different intensities of pulsed ultrasound exposure on the fibrinolytic properties of streptokinase and reteplase. Measurements were performed both following pre-exposure of the drugs and clots to pulsed ultrasound, and following concomitant exposure of clots an...

  1. Isolation and Characterization of a 32-kDa Fibrinolytic Enzyme (FE-32kDa from Gloydius blomhoffii siniticus Venom

    Directory of Open Access Journals (Sweden)

    Kim Joung-Yoon

    2014-03-01

    Full Text Available Objectives: This study was undertaken to isolate a fibrinolytic enzyme from the snake venom of Gloydius blomhoffii siniticus and to investigate its enzymatic characteristics and hemorrhagic activity as a potential pharmacopuncture agent. Methods: The fibrinolytic enzyme was isolated by using chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and fibrin plate assay. The characteristics of the enzyme were investigated using fibrin plate assay, protein hydrolysis analysis, and hemorrhage assay. Its amino acid composition was determined. Results: The fibrinolytic enzyme with the molecular weight of 32kDa (FE-32kDa from Gloydius blomhoffii siniticus showed a fibrin hydrolysis zone at the concentration of 0.2 mg/mL in the fibrin plate assay. The fibrin hydrolysis activity of the enzyme was inhibited completely by ethylenediaminetetraacetic acid (EDTA, ethyleneglycoltetraacetic acid (EGTA, and 1, 10-phenanthroline, thiothreitol and cysteine, and partially by phenylmethanesulfonylfluoride (PMSF. Metal ions such as Fe2+ and Hg2+ inhibited the fibrin hydrolysis completely, but Zn2+ enhanced it. FE-32kDa hydrolyzed α-chain but did not hydrolyze β-chain and γ-chain of fibrinogen. High-molecular-weight polypeptides of gelatin were hydrolyzed partially into low-molecular-weight polypeptides, but the extent of hydrolysis was limited. FE-32kDa induced hemorrhage beneath back skin of mice at the dose of 2 μg. Conclusions: FE-32kDa is a α-fibrin(ogenolytic metalloprotease that requires Zn2+ for fibrinolytic activity and causes hemorrhage, suggesting that the enzyme is not appropriate for use as a clinical pharmacopuncture.

  2. Haemorrheologic and fibrinolytic activities in diabetics resident in ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-05-16

    May 16, 2008 ... complication of retinopathy, nephropathy, and neuropathy .... of diabetic patients were significantly (P<0.05) higher than that of the control .... knowledge, attitude, practice and belief of type 2 Nigerian diabetes patients on drug ...

  3. Enzymological characterization of Flla, a fibrinolytic enzyme from Agkistrodon acutus venom

    Institute of Scientific and Technical Information of China (English)

    Xiu-xia LIANG; Ying-na ZHOU; Jia-shu CHEN; Peng-xin QIU; Hui-zhen CHEN; Huan-huan SUN; Yu-ping WU; Guang-mei YAN

    2005-01-01

    Aim: To study the enzymological characterization of a fibrinolytic enzyme (FⅡa)from Agkistrodon acutus venom. Methods: The fibrinogenolytic effect and the influences of several protease inhibitors, chelating agents, and metal ions on fibrinogenolytic activity were visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The metal content of FⅡa was determined by atomic absorption spectroscopy. Results: After incubation with EⅡa (0.25 g/L), Aα-, Bβ-and γ-chains of fibrinogen disappeared within 5 min, 30 min, and 8 h, respectively.The molecular weights of major degradation products were 45 000 and 41 000,which were different from those bands produced by plasmin. The fibrinogenolytic activity of FⅡa was strongly inhibited by ethylenediamine tetraacetic acid (EDTA),ethyleneglycol tetraacetic acid (EGTA), dithiothreitol and cysteine, but not by phenylmethyl-sulfonyl fluoride and soybean trypsin inhibitor. Zinc (3171±25mg/kg), potassium (489± 17 mg/kg) and calcium (319± 13 mg/kg) were found in FⅡa.Zn2+, Ca2+ and Mg2+ could recover the fibrinogenolytic activity of FⅡa, which was inhibited by EDTA. Only Ca2+ could recover the fibrinogenolytic activity inhibited by EGTA. Conclusion: FⅡa can degrade the Aα-, Bβ- and γ-chains of fibrinogen.EⅡa is a metalloproteinase, and Zn2+, Ca2+, and disulfide bonds are necessary for its fibrinogenolytic activity.

  4. Oceanographic profile data collected from CTD and sound velocimeter - moving vessel profiler casts aboard FA2805, FA2806, FA2807, FA2808 and NOAA Ship FAIRWEATHER as part of project OPR-O190-FA-15 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2015-09-23 to 2015-11-11 (NCEI Accession 0138176)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0138176 includes physical and profile data collected aboard the FA2805, FA2806, FA2807, FA2808 and NOAA Ship FAIRWEATHER during project OPR-O190-FA-15...

  5. Oceanographic profile data collected from CTD casts aboard FA2805, FA2806, FA2807, FA2808 and FAIRWEATHER as part of project OPR-P335-FA-16 in the Gulf of Alaska from 2016-06-24 to 2016-08-01 (NCEI Accession 0156699)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0156699 includes physical and profile data collected aboard the FA2805, FA2806, FA2807, FA2808 and FAIRWEATHER during project OPR-P335-FA-16 in the...

  6. Oceanographic profile data collected from CTD casts aboard FA2805, FA2806, FA2807, FA2808 and NOAA Ship FAIRWEATHER as part of project OPR-O190-FA-16 in the Coastal Waters of SE Alaska from 2016-05-08 to 2016-06-15 (NCEI Accession 0155203)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155203 includes physical and profile data collected aboard the FA2805, FA2806, FA2807, FA2808 and NOAA Ship FAIRWEATHER during project OPR-O190-FA-16...

  7. Oceanographic profile data collected from CTD casts aboard FA2805, FA2806, FA2807, FA2808 and NOAA Ship FAIRWEATHER as part of project OPR-S327-FA-15 in the Beaufort Sea and Bering Sea from 2015-06-25 to 2015-08-14 (NCEI Accession 0131204)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0131204 includes physical and profile data collected aboard the FA2805, FA2806, FA2807, FA2808 and NOAA Ship FAIRWEATHER during project OPR-S327-FA-15...

  8. Effect of façade impregnation on feasibility of capillary active thermal internal insulation for a historic dormitory – A hygrothermal simulation study

    DEFF Research Database (Denmark)

    Finken, Gholam Reza; Bjarløv, Søren Peter; Peuhkuri, Ruut Hannele

    2016-01-01

    Internal insulation of external walls is known to create moisture performance challenges due to increased moisture levels and condensation risk on the cold side of the insulation. Capillary active/hydrophilic insulations have been introduced to solve these moisture problems, since they are able t...

  9. Effect of ascorbate on fibrinolytic factors in septic mouse skeletal muscle.

    Science.gov (United States)

    Swarbreck, Scott; Secor, Dan; Li, Fuyan; Gross, Peter L; Ellis, Christopher G; Sharpe, Michael D; Wilson, John X; Tyml, Karel

    2014-10-01

    Plugging of the capillary bed in tissues correlates with organ failure during sepsis. In septic mouse skeletal muscle, we showed that blood in capillaries becomes hypercoagulable and that ascorbate injection inhibits capillary plugging. In the present study, we hypothesized that ascorbate promotes fibrinolysis, reversing this plugging. Sepsis in mice was induced by fecal injection into peritoneum. Mice were injected intravenously with a bolus of streptokinase (fibrinolytic agent) or ascorbate at 5-6 h. Both agents reversed capillary plugging in muscle at 7 h. Sepsis increased mRNA expression of urokinase plasminogen activator (u-PA) (profibrinolytic) and plasminogen activator inhibitor 1 (PAI-1) (antifibrinolytic) in muscle and liver homogenates at 7 h. Ascorbate did not affect u-PA mRNA in either tissue, but it inhibited PAI-1 mRNA in muscle, suggesting enhanced fibrinolysis in this tissue. However, ascorbate did not affect increased PAI-1 mRNA in the liver (dominant source of soluble PAI-1 in systemic blood). Consistently, ascorbate affected neither elevated PAI-1 protein/enzymatic activity in septic liver nor lowered plasmin antiplasmin level in septic blood. Furthermore, hypocoagulability of septic blood revealed by thrombelastography and thrombin-induced PAI-1 release from isolated platelets (ex-vivo model of sepsis) were not affected by ascorbate. Based on the PAI-1 protein data, the present study does not support the hypothesis that ascorbate promotes fibrinolysis in sepsis.

  10. Arsenic exposure disrupts the normal function of the FA/BRCA repair pathway.

    Science.gov (United States)

    Peremartí, Jana; Ramos, Facundo; Marcos, Ricard; Hernández, Alba

    2014-11-01

    Chronic arsenic exposure is known to enhance the genotoxicity/carcinogenicity of other DNA-damaging agents by inhibiting DNA repair activities. Interference with nucleotide excision repair and base excision repair are well documented, but interactions with other DNA repair pathways are poorly explored so far. The Fanconi anemia FA/BRCA pathway is a DNA repair mechanism required for maintaining genomic stability and preventing cancer. Here, interactions between arsenic compounds and the FA/BRCA pathway were explored by using isogenic FANCD2(-/-) (FA/BRCA-deficient) and FANCD2(+/+) (FA/BRCA-corrected) human fibroblasts. To study whether arsenic disrupts the normal FA/BRCA function, FANCD2(+/+) cells were preexposed to subtoxic concentrations of the trivalent arsenic compounds methylarsonous acid (MMA(III)) and arsenic trioxide (ATO) for 2 weeks. The cellular response to mitomicin-C, hydroxyurea, or diepoxybutane, typical inducers of the studied pathway, was then evaluated and compared to that of FANCD2(-/-) cells. Our results show that preexposure to the trivalent arsenicals MMA(III) and ATO induces in corrected cells, a cellular FA/BRCA-deficient phenotype characterized by hypersensitivity, enhanced accumulation in the G2/M compartment and increased genomic instability--measured as micronuclei. Overall, our data demonstrate that environmentally relevant arsenic exposures disrupt the normal function of the FA/BRCA activity, supporting a novel source of arsenic co- and carcinogenic effects. This is the first study linking arsenic exposure with the FA/BRCA DNA repair pathway.

  11. FANCI Regulates Recruitment of the FA Core Complex at Sites of DNA Damage Independently of FANCD2.

    Directory of Open Access Journals (Sweden)

    Maria Castella

    2015-10-01

    Full Text Available The Fanconi anemia (FA-BRCA pathway mediates repair of DNA interstrand crosslinks. The FA core complex, a multi-subunit ubiquitin ligase, participates in the detection of DNA lesions and monoubiquitinates two downstream FA proteins, FANCD2 and FANCI (or the ID complex. However, the regulation of the FA core complex itself is poorly understood. Here we show that the FA core complex proteins are recruited to sites of DNA damage and form nuclear foci in S and G2 phases of the cell cycle. ATR kinase activity, an intact FA core complex and FANCM-FAAP24 were crucial for this recruitment. Surprisingly, FANCI, but not its partner FANCD2, was needed for efficient FA core complex foci formation. Monoubiquitination or ATR-dependent phosphorylation of FANCI were not required for the FA core complex recruitment, but FANCI deubiquitination by USP1 was. Additionally, BRCA1 was required for efficient FA core complex foci formation. These findings indicate that FANCI functions upstream of FA core complex recruitment independently of FANCD2, and alter the current view of the FA-BRCA pathway.

  12. Physiological Effect of New FA Antitranspirant Application on Winter Wheat at Ear Filling Stage

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The physiological effect of new FA antitranspirant on winter wheat was studied by field trial. The new FA antitranspirant was sprayed at ear filling stage, using the following concentrations: 0.5, 1.0, 1.5 and 2.0 mL L-1.The results indicated that new FA antitranspirant increased nitrate reductase activity (NRA), free proline content, chlorophyll content and water content of leaf, thus drought stress can be mitigated. The new FA antitranspirant increased photosynthesis, enlarged stomatal conductance and reduced transpiration rate, thus led to growth stimulation and water loss reduction. New FA antitranspirant caused an increase of grain yield by 7.2%, under the optimal concentration 1.5 mL L-1.

  13. Blood compatibility of a ferulic acid (FA)-eluting PHBHHx system for biodegradable magnesium stent application.

    Science.gov (United States)

    Zhang, Erlin; Shen, Feng

    2015-01-01

    Magnesium stent has shown potential application as a new biodegradable stent. However, the fast degradation of magnesium stent limited its clinic application. Recently, a biodegradable and drug-eluting coating system was designed to prevent magnesium from fast degradation by adding ferulic acid (FA) in poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by a physical method. In vitro study has demonstrated that the FA-eluting system exhibited strong promotion to the endothelialization, which might be a choice for the stent application. In this paper, the hemolysis rate, the plasma recalcification time (PRT), the plasma prothrombin time (PT) and the kinetic clotting time of the FA-eluting films were investigated and the platelet adhesion was observed in order to assess the blood compatibility of the FA-eluting PHBHHx films in comparison with PHBHHx film. The results have shown that the addition of FA had no influence on the hemolysis, but prolonged PRT, PT and the clotting time and reduced the platelet adhesion and activation, displaying that the FA-eluting PHBHHx exhibited better blood compatibility than PHBHHx. In addition, the effect of alkali treatment on the blood compatibility of FA-eluting PHBHHx was also studied. It was indicated that alkali treatment had no effect on the hemolysis and the coagulation time, but enhanced slightly the platelet adhesion. All these demonstrated that FA-eluting PHBHHx film had good blood compatibility and might be a candidate surface coating for the biodegradable magnesium stent.

  14. Co-operation between interleukin-1 and the fibrinolytic system in the degradation of collagen by articular chondrocytes.

    Science.gov (United States)

    Cruwys, S C; Davies, D E; Pettipher, E R

    1990-07-01

    1. The interaction between interleukin 1 (IL-1) and the fibrinolytic system in the control of collagen degradation by rabbit chondrocytes has been investigated in a tissue-culture system where cells are grown on a 14C-labelled collagen matrix. 2. Culture of rabbit chondrocytes in the presence of human recombinant IL-1 beta at a concentration of 57pM for 48 h led to the presence of procollagenase but not active collagenase in the medium. The latent collagenase could be activated by incubation with an organomercurial, aminophenylmercuric acetate (APMA). 3. Addition of IL-1 beta to chondrocytes grown on a 14C-labelled collagen matrix did not increase the degradation of the matrix compared to control over a 48 h period. However, in the presence of plasmin (200 micrograms ml-1) or plasminogen (100 micrograms ml-1), IL-1 beta (57 pM) caused almost complete degradation of the collagen matrix. Plasmin or plasminogen alone caused only slight degradation of the collagen matrix. 4. Tissue inhibitor of metalloproteinases (TIMP) or the selective metalloproteinase inhibitor, SC44463, inhibited the degradation induced by IL-1 beta and plasminogen in a concentration-related manner and at concentrations that were correlated with inhibition of collagenase. 5. When concentrations of IL-1 beta which caused only minimal degradation of the matrix in the presence of plasminogen were combined with fibrin (1 microgram ml-1), there was almost total degradation of the matrix by 48 h. 6. These results indicate there is a synergistic interaction between IL-1 and the fibrinolytic system in the degradation of collagen by rabbit chondrocytes in culture.

  15. International Façades - CROFT: Climate Related Optimized Façade Technologies

    NARCIS (Netherlands)

    Bilow, M.

    2012-01-01

    "International Façades - CROFT" links the fields of architecture, building services and building physics. It aims at an international diversity in façade design to reduce energy consumption in building design. Current architectural planning practices, such as the International Style, perceive the c

  16. The Effect of Levonorgestrel on Fibrinolytic Factors in Human Endometrial Endothelial Cells.

    Science.gov (United States)

    Pakrashi, Tarita; Taylor, Joelle E; Nelson, Ashley; Archer, David F; Jacot, Terry

    2016-11-01

    The levonorgestrel-releasing intrauterine system is considered a highly effective treatment of heavy menstrual bleeding (HMB). While LNG has established effects on the stromal and glandular compartments of the endometrial tissue, its effect on the endometrial endothelial cells has not been investigated. We examined whether LNG regulates fibrinolytic factors, tissue plasminogen activator (tPA), and urokinase plasminogen activator (uPA) secreted by human endometrial endothelial cells (HEECs) and determined the steroid receptor through which LNG exerts its effect on the endothelium. The HEECs were treated with LNG or progesterone and levels of tPA and plasminogen activator inhibitor 1 (PAI-1) measured. The HEECs were specifically examined for the presence of androgen receptors through Western blot. Levonorgestrel ± flutamide were added to HEECs and the levels of tPA and uPA were examined. An enzyme-linked immunosorbent assay performed on culture media confirmed a statistically significant decrease in tPA levels in cells treated with LNG (77.80% ± 8.0% of control; n = 5, P < .05 vs control) but not progesterone. The androgen receptor (110 kDa) was detected in HEEC lysates. The decrease in tPA was blocked by the addition of flutamide (101.3% ± 16% of control), a classic nonsteroidal androgen receptor blocker. There was no change in uPA or PAI-1 levels in cells treated with LNG. Levonorgestrel decreases tPA levels through the androgen receptor in HEECs. Thus, LNG inhibits tPA secretion by the endometrial endothelial cell. This response suggests reduction in HMB with LNG-IUS could reflect an LNG-mediated promotion of hemostasis. © The Author(s) 2016.

  17. Designing Urban Media Façades

    DEFF Research Database (Denmark)

    Dalsgaard, Peter; Halskov, Kim

    2010-01-01

    Media façades comprise a category of urban computing concerned with the integration of displays into the built environment, including buildings and street furniture. This paper identifies and discusses eight challenges faced when designing urban media façades. The challenges concern a broad range...... of issues: interfaces, physical integration, robustness, content, stakeholders, situation, social relations, and emerging use. The challenges reflect the fact that the urban setting as a domain for interaction design is characterized by a number of circumstances and socio-cultural practices that differ from...

  18. The fibrinolytic system in the hemolytic uremic syndrome: In vivo and in vitro studies

    NARCIS (Netherlands)

    Kar, N.C.A.J. van de; Hinsbergh, V.W.M. van; Brommer, E.J.P.; Monnens, L.A.H.

    1994-01-01

    Fibrinolytic parameters and von Willebrand factor (vWF) antigen were measured in the plasma of 10 patients with hemolytic uremic syndrome (HUS). Samples were taken at presentation and again 2 wk later, before and after infusion of 1-desamino-8-arginine vasopressin. Compared with the plasma values of

  19. Effect of low-dose aspirin during pregnancy on fibrinolytic variables before and after parturition

    NARCIS (Netherlands)

    H.A. Bremer (Henk); N. Rotmans (Nel); E.J.Ph. Brommer; H.C.S. Wallenburg (Henk)

    1995-01-01

    textabstractOBJECTIVE: We assessed the effects of a daily oral dose of 60 to 80 mg of aspirin from 12 weeks gestation until delivery on fibrinolytic variables before and after parturition. STUDY DESIGN: In a prospective controlled study labor was electively induced in 24 patients, eight receiving

  20. 可口革囊星虫(Phascolosoma esculenta)纤溶酶PFE的分离纯化%Study on fibrinolytic enzyme from Phascolosoma Esculenta

    Institute of Scientific and Technical Information of China (English)

    牛荣丽; 熊亚楠; 吕佳美悦

    2011-01-01

    Objective To find a thrombolytic substance from Phascolosoma esculenta. Methods Detecting the fibrinolytic activity by fibrin plate method, the fibrinolytic enzyme from Phascolosoma esculenta was extracted by homogenization, centrifugation and purification by gel filtration column G-25 and DEAE. Then characterizations of the enzyme activity were observed including Ph, temperature, metal ion and inhibitors. Results The fibrinolytic enzyme from Phascolosoma esculenta was isolated and named PFE, which relative molecular mass was about 35 Kd. PFE could degrade directly fibrin. The optimal Ph of PFE was 7. 4, which activity was keep stable between Ph6. 6~9. 0 or the temperature 20~40 °C. The activity of PFE was inhibited by Ca2+、Mg2+、Fe2+、K+、EDTA and β-mercaptoethanol, esp. Strongly inhibited by Fe3+. Conclusion There was fibrinolytic enzyme which can directly degrade fibrin in Phascolosoma esculenta.%目的 从可口革囊星虫中分离纯化纤溶酶,并初步研究其酶学性质.方法 实验以纤维蛋白平板法检测纤溶活性,采用匀浆、抽提离心、G-25脱盐、DEAE梯度洗脱等方法从可口革囊星虫中分离纯化出纤溶酶PFE,并进一步研究pH、温度、金属离子及抑制剂对该酶活性的影响.结果 分离纯化得到纤溶酶PFE,SDSPAGE法测得相对分子质量为35 kDa左右.此外,其酶学性质研究结果表明:PFE的最适反应pH为7.4,酶活力在pH6.6~9.0时相对稳定;PFE在20~40℃条件下,酶活力保持相对稳定.Fe3+对酶活性有强烈的抑制作用,Ca2+、Mg2+、Fe2+、K+、EDTA和β-巯基乙醇对酶活性均具有一定的抑制作用.结论 可口革囊星虫中存在纤溶酶PFE,此酶可直接降解纤维蛋白,具有重要的临床开发价值.

  1. Comparative fibrinolytic properties of staphylokinase and streptokinase in animal models of venous thrombosis.

    Science.gov (United States)

    Lijnen, H R; Stassen, J M; Vanlinthout, I; Fukao, H; Okada, K; Matsuo, O; Collen, D

    1991-10-01

    The thrombolytic and pharmacokinetic properties of staphylokinase were compared with those of streptokinase in hamsters with a pulmonary embolus produced from human plasma or from hamster plasma, and in rabbits with a jugular vein blood clot produced from rabbit blood. In both models, a continuous intravenous infusion of staphylokinase and streptokinase over 60 min in hamsters or over 4 h in rabbits, induced dose-dependent progressive clot lysis in the absence of significant systemic activation of the fibrinolytic system. The results of thrombolytic potency (clot lysis at 30 min after the end of the infusion, in percent, versus dose administered, in mg/kg) were fitted with an exponentially transformed sigmoidal function (formula: see text) and the maximal percent clot lysis (c), the maximal rate of lysis (z = 1/4 ac.eb) and the dose at which the maximal rate of lysis is achieved (b) were determined. In hamsters with a pulmonary embolus produced from human plasma, streptokinase had a somewhat higher thrombolytic potency than staphylokinase, as revealed by a higher z value (2,100 +/- 1,100% lysis per mg/kg streptokinase administered versus 1,100 +/- 330% lysis per mg/kg for staphylokinase). In hamsters with a pulmonary embolus produced from hamster plasma, staphylokinase had a somewhat higher thrombolytic potency than streptokinase (z = 1,600 +/- 440 versus 1,200 +/- 370% lysis per mg/kg). Staphylokinase had a higher thrombolytic potency than streptokinase in rabbits,as revealed by a higher z-value (950 +/- 350% lysis per mg/kg staphylokinase administered versus 330 +/- 39% lysis per mg/kg for streptokinase) and a lower b-value (0.035 +/- 0.010 mg/kg staphylokinase versus 0.091 +/- 0.008 mg/kg for streptokinase.(ABSTRACT TRUNCATED AT 250 WORDS)

  2. Opuntia ficus indica (nopal) attenuates hepatic steatosis and oxidative stress in obese Zucker (fa/fa) rats.

    Science.gov (United States)

    Morán-Ramos, Sofía; Avila-Nava, Azalia; Tovar, Armando R; Pedraza-Chaverri, José; López-Romero, Patricia; Torres, Nimbe

    2012-11-01

    Nonalcoholic fatty liver disease (NAFLD) is associated with multiple factors such as obesity, insulin resistance, and oxidative stress. Nopal, a cactus plant widely consumed in the Mexican diet, is considered a functional food because of its antioxidant activity and ability to improve biomarkers of metabolic syndrome. The aim of this study was to assess the effect of nopal consumption on the development of hepatic steatosis and hepatic oxidative stress and on the regulation of genes involved in hepatic lipid metabolism. Obese Zucker (fa/fa) rats were fed a control diet or a diet containing 4% nopal for 7 wk. Rats fed the nopal-containing diet had ∼50% lower hepatic TG than the control group as well as a reduction in hepatomegaly and biomarkers of hepatocyte injury such as alanine and aspartate aminotransferases. Attenuation of hepatic steatosis by nopal consumption was accompanied by a higher serum concentration of adiponectin and a greater abundance of mRNA for genes involved in lipid oxidation and lipid export and production of carnitine palmitoyltransferase-1 and microsomal TG transfer proteins in liver. Hepatic reactive oxygen species and lipid peroxidation biomarkers were significantly lower in rats fed nopal compared with the control rats. Furthermore, rats fed the nopal diet had a lower postprandial serum insulin concentration and a greater liver phosphorylated protein kinase B (pAKT):AKT ratio in the postprandial state. This study suggests that nopal consumption attenuates hepatic steatosis by increasing fatty acid oxidation and VLDL synthesis, decreasing oxidative stress, and improving liver insulin signaling in obese Zucker (fa/fa) rats.

  3. Fibrinolytic, anti-inflammatory and anti-microbial properties of α-(1-3)-glucans produced from Streptococcus mutans (MTCC 497).

    Science.gov (United States)

    Buddana, Sudheer Kumar; Varanasi, Yaswanth Venkata Naga; Shetty, Prakasham Reddy

    2015-01-22

    Streptococcus mutans (MTCC 497) cell associated α-(1-3)-glucans were isolated, characterized and evaluated for their bioactivity profile. Acid hydrolysis of α-(1-3)-glucans revealed presence of glucose moieties. Water insoluble α-(1-3)-glucans (WIG) were sulfated to convert them into water soluble glucans which were characterized by FT-IR spectral studies. The sulfation of WIG was confirmed by the presence of -O-SO3- and C-O-SO3- characteristic peaks at 1240 and 820 cm(-1). MALDI-TOF analysis of sulfated α-(1-3)-glucan revealed 1.2 to 9kDa fragmentation. Antibacterial profile studies revealed higher growth inhibitory activity against Gram negative than Gram positive bacterial strains by sulfated α-(1-3)-glucans. One-fold higher anti-inflammatory activity with IC50 value of 0.11mg/ml was observed with sulfated α-(1-3)-glucans over WIG. Time dependent fibrinolytic potential without requirement of tissue plasminogen activators was observed for sulfated α-(1-3)-glucans. This is the first report demonstrating the fibrinolytic and anti-inflammatory property for sulfated α-(1-3)-glucans.

  4. Molecular Cloning and Characterization of cDNA Encoding Fibrinolytic Enzyme-3 from Earthworm Eisenia foetida

    Institute of Scientific and Technical Information of China (English)

    Guo-Qing DONG; Xiao-Ling YUAN; Ya-Jun SHAN; Zhen-Hu ZHAO; Jia-Pei CHEN; Yu-Wen CONG

    2004-01-01

    The earthworm fibrinolytic enzyme-3 (EFE-3, GenBank accession No: AY438622), from the earthworm Eiseniafoetida, is a component of earthworm fibrinolytic enzymes. In this study, cDNA encoding the EFE-3 was cloned by RT-PCR. The eDNA contained an open reading frame of 741 nucleotides, which encoded a deduced protein of 247 amino acid residues, including signal sequences. EFE-3 showed a high degree of homology to earthworm (Lumbricus rebullus) proteases F-III-1, F-III-2, and bovine trypsin. The recombinant EFE-3 was expressed in E. coli as inclusion bodies, and the gene encoding the native form of EFE-3 was expressed in COS-7 cells in the medium. Both the refolding product of inclusion bodies and the secreted protease could dissolve the artificial fibrin plate.

  5. Polymorphic variation of genes in the fibrinolytic system and the risk of ovarian cancer.

    Directory of Open Access Journals (Sweden)

    Yaakov Bentov

    Full Text Available INTRODUCTION: The etiology of ovarian cancer is largely unknown. One hypothesis is that the inefficient removal of the blood clots and fibrin products which are deposited in the vicinity of the ovary by retrograde menstruation might be associated with an increased risk of ovarian cancer. Several single nucleotide polymorphisms within genes which comprise the fibrinolytic system have been shown to have functional effects on the rate of blood clot degradation. These were considered to be candidate genes in the present study. AIM: We studied the genotype distributions of 12 functional SNPs of four genes (tPA, uPA PAI1 and TAFI among 775 ovarian cancer cases and 889 controls. RESULTS: No significant associations were seen between any of the ten SNPs and the risk of ovarian cancer as a whole, or in any histologic subgroup. DISCUSSION: Germline known functional variants of genes in the fibrinolytic system are not associated with risk of ovarian cancer.

  6. Spontaneous Hemarthrosis Following Fibrinolytic Therapy for Acute Myocardial Infarction: A Case Report and Literature Review

    OpenAIRE

    Mahmoud M. Ramadan; Khan, Iqbal S.; Mahdi, Ousama

    2014-01-01

    Patient: Male, 48 Final Diagnosis: Spontaneous hemarthrosis of right knee Symptoms: A rapidly growing knee swelling was “witnessed” associated with severe tenderness, hotness and profound agony but without color change Medication: — Clinical Procedure: Suprapatellar arthrocentesis of the right knee joint Specialty: Cardiology Objective: Unusual clinical course Background: Despite the widespread use of fibrinolytic therapy and the numerous reports on its bleeding complications, spontaneous hem...

  7. Pharmacokinetics and pharmacodynamics of tenecteplase in fibrinolytic therapy of acute myocardial infarction.

    Science.gov (United States)

    Tanswell, Paul; Modi, Nishit; Combs, Dan; Danays, Thierry

    2002-01-01

    Tenecteplase is a novel fibrinolytic protein bioengineered from human tissue plasminogen activator (alteplase) for the therapy of acute ST-segment elevation myocardial infarction. Specific mutations at three sites in the alteplase molecule result in 15-fold higher fibrin specificity, 80-fold reduced binding affinity to the physiological plasminogen activator inhibitor PAI-1 and 6-fold prolonged plasma half-life (22 vs 3.5 minutes). Consequently, tenecteplase can be administered as a single intravenous bolus of 30-50mg (0.53 mg/kg bodyweight) over 5-10 seconds, in contrast to the 90-minute accelerated infusion regimen of alteplase. Tenecteplase plasma concentration-time profiles have been obtained from a total of 179 patients with acute myocardial infarction. Tenecteplase exhibited biphasic disposition; the initial disposition phase was predominant with a mean half-life of 17-24 minutes, and the mean terminal half-life was 65-132 min. Over the clinically relevant dose range of 30-50mg, mean clearance (CL) was 105 ml/min. The mean initial volume of distribution V(1) was 4.2-6.3L, approximating plasma volume, and volume of distribution at steady state was 6.1-9.9L, suggesting limited extravascular distribution or binding. Bodyweight and age were found to influence significantly both CL and V(1). Total bodyweight explained 19% of the variability in CL and 11% of the variability in V(1), and a 10kg increase in total bodyweight resulted in a 9.6 ml/min increase in CL. This relationship aided the development of a rationale for the weight-adjusted dose regimen for tenecteplase. Age explained only a further 11% of the variability in CL. The percentage of patients who achieved normal coronary blood flow was clearly related to AUC. More than 75% of patients achieved normal flow at 90 minutes after administration when their partial AUC(2-90) exceeded 320 microg.min/ml, corresponding to an average plasma concentration of 3.6 microg/ml. Systemic exposure to tenecteplase at all

  8. Excessive food intake, obesity and inflammation process in Zucker fa/fa rat pancreatic islets.

    Directory of Open Access Journals (Sweden)

    Myriam Chentouf

    Full Text Available Inappropriate food intake-related obesity and more importantly, visceral adiposity, are major risk factors for the onset of type 2 diabetes. Evidence is emerging that nutriment-induced β-cell dysfunction could be related to indirect induction of a state of low grade inflammation. Our aim was to study whether hyperphagia associated obesity could promote an inflammatory response in pancreatic islets leading to ß-cell dysfunction. In the hyperphagic obese insulin resistant male Zucker rat, we measured the level of circulating pro-inflammatory cytokines and estimated their production as well as the expression of their receptors in pancreatic tissue and β-cells. Our main findings concern intra-islet pro-inflammatory cytokines from fa/fa rats: IL-1β, IL-6 and TNFα expressions were increased; IL-1R1 was also over-expressed with a cellular redistribution also observed for IL-6R. To get insight into the mechanisms involved in phenotypic alterations, abArrays were used to determine the expression profile of proteins implicated in different membrane receptors signaling, apoptosis and cell cycle pathways. Despite JNK overexpression, cell viability was unaffected probably because of decreases in cleaved caspase3 as well as in SMAC/DIABLO and APP, involved in the induction and amplification of apoptosis. Concerning β-cell proliferation, decreases in important cell cycle regulators (Cyclin D1, p35 and increased expression of SMAD4 probably contribute to counteract and restrain hyperplasia in fa/fa rat islets. Finally and probably as a result of IL-1β and IL-1R1 increased expressions with sub-cellular redistribution of the receptor, islets from fa/fa rats were found more sensitive to both stimulating and inhibitory concentrations of the cytokine; this confers some physiopathological relevance to a possible autocrine regulation of β-cell function by IL-1β. These results support the hypothesis that pancreatic islets from prediabetic fa/fa rats undergo an

  9. Liver oxidation and inflammation in Fa/Fa rats fed glucomannan/spirulina-surimi.

    Science.gov (United States)

    Vázquez-Velasco, Miguel; González-Torres, Laura; López-Gasco, Patricia; Bastida, Sara; Benedí, Juana; Sánchez-Reus, María Isabel; González-Muñoz, María José; Sánchez-Muniz, Francisco J

    2014-09-15

    The effect of high-fat squid-surimi diets enriched in glucomannan or glucomannan-spirulina on lipemia, liver glutathione status, antioxidant enzymes and inflammation biomarkers was determined in Zucker Fa/Fa rats. Groups of eight rats each received for 7weeks the squid-surimi control (C), glucomannan-enriched squid-surimi (G) and glucomannan-spirulina enriched squid-surimi (GS). Liver weight, cytochrome P450 7A1 expression and cholesterolemia were decreased in G and GS vs. C, improving glutathione red-ox index (pspirulina kept those hypocholesterolemic and antioxidant effects but reduced the inflammation observed. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Excessive food intake, obesity and inflammation process in Zucker fa/fa rat pancreatic islets.

    Science.gov (United States)

    Chentouf, Myriam; Dubois, Gregor; Jahannaut, Céline; Castex, Françoise; Lajoix, Anne Dominique; Gross, René; Peraldi-Roux, Sylvie

    2011-01-01

    Inappropriate food intake-related obesity and more importantly, visceral adiposity, are major risk factors for the onset of type 2 diabetes. Evidence is emerging that nutriment-induced β-cell dysfunction could be related to indirect induction of a state of low grade inflammation. Our aim was to study whether hyperphagia associated obesity could promote an inflammatory response in pancreatic islets leading to ß-cell dysfunction. In the hyperphagic obese insulin resistant male Zucker rat, we measured the level of circulating pro-inflammatory cytokines and estimated their production as well as the expression of their receptors in pancreatic tissue and β-cells. Our main findings concern intra-islet pro-inflammatory cytokines from fa/fa rats: IL-1β, IL-6 and TNFα expressions were increased; IL-1R1 was also over-expressed with a cellular redistribution also observed for IL-6R. To get insight into the mechanisms involved in phenotypic alterations, abArrays were used to determine the expression profile of proteins implicated in different membrane receptors signaling, apoptosis and cell cycle pathways. Despite JNK overexpression, cell viability was unaffected probably because of decreases in cleaved caspase3 as well as in SMAC/DIABLO and APP, involved in the induction and amplification of apoptosis. Concerning β-cell proliferation, decreases in important cell cycle regulators (Cyclin D1, p35) and increased expression of SMAD4 probably contribute to counteract and restrain hyperplasia in fa/fa rat islets. Finally and probably as a result of IL-1β and IL-1R1 increased expressions with sub-cellular redistribution of the receptor, islets from fa/fa rats were found more sensitive to both stimulating and inhibitory concentrations of the cytokine; this confers some physiopathological relevance to a possible autocrine regulation of β-cell function by IL-1β. These results support the hypothesis that pancreatic islets from prediabetic fa/fa rats undergo an inflammatory

  11. Deterministic Assessment of Future Costs for Dismantling (FA)

    Energy Technology Data Exchange (ETDEWEB)

    Vasko, Marek [DECOM, Trnava (Slovakia)

    2012-11-01

    The main objective of the report is to provide an re-evaluation of cost calculations by OMEGA code for the Intermediate Storage for Spent Fuel in Studsvik (FA facility) using up-to-date Swedish labour cost unit factors and available up-to-date Swedish (or international) cost unit factors for consumables, materials and substances. Furthermore, evolution of other OMEGA database parameters concerning cost calculations e.g. manpower unit factors and workgroups parameters are taken into account. This report follows up former project which introduced tentative calculations of main decommissioning parameters such as costs, manpower and exposure of personnel for activities of older nuclear facility decommissioning in Sweden represented by FA Facility in Studsvik by means of calculation code OMEGA. The project demonstrated an implementation of advanced costing methodology based on PSL structure format to achieve transparent, traceable and comparable estimates even for older nuclear facilities like FA Facility in Studsvik. This former project used Slovak origin labour costs unit factors and other cost unit factors. After successful completion of this project, there was an intent of SSM to reevaluate calculations using an up-to-date Swedish labour cost data and also available Swedish consumables and materials cost data if available. Within this report re-calculations of main decommissioning parameters using available Swedish data are presented in structure according to Proposed Standardized List of Items for Costing Purposes. Calculations are made for decommissioning scenario with post-dismantling decontamination and steel radwaste melting technologies available at the site. All parameters are documented and summed up in both table and graphic forms in text and Annexes. Further, comparison of calculated results with previous calculations together with discussion is provided.

  12. 华广虻肠道溶纤活性蛋白的纯化和性质%CHARACTERIZATION AND PURIFICATION OF GUT FIBRINOLYTIC PROTEASE FROM TABANUS AMAENUS

    Institute of Scientific and Technical Information of China (English)

    杨星勇; 卢晓风; 程惊秋; 裴炎

    2001-01-01

    After ammonium sulphate precipitation, Sephadex G-75 gel filtration, Lys-Sepharose 4B affinity chromatography and elution from electrophoresis, the fibrinolytic protease (TAFP) was isolated and purified from the extract of T. amaenus Walker gut. It appeared a single band corresponding to molecular weight of approximately 67kD on SDS-PAGE and an probably pI of 7.2 on IEF. On fibrin plate and plasminogen-free fibrin plate (heated at 85℃ for 30 minutes to eliminate plasminogen), TAFP showed same fibrinolytic activity. The result might indicate that TAFP is a fibrinolytic enzyme degrading fibrin, as well as a plasminogen activator degrading fibrin via activating plasminogen. The result of chromogenic substrates indicated that TAFP possesses trypsin-like activity specifically degrading argininyl amide bond or peptide bond, but has no chymotrypsin activity. TAFP was almost inhibited powerfully by antipain, PMSF, soybean trypsin inhibitor and soybean Bowman-Birk inhibitor. However, leupeptin, antitrypsin and TLCK was more powerful effective inhibitors of TAFP. Optimal reaction pH of TAFP was 7.5, and it was stable in 5.5 - 7.0 of pH range.%经过75%饱和度硫酸铵沉淀、Sephadex G-75凝胶过滤层析、Lys-Sepharose 4B亲和层析和电泳制备洗脱,从华广虻(Tabanus amaenus Walker)腹部组织匀浆液中分离纯化出分子量约为67KD的溶纤活性蛋白TAFP.经纤维蛋白平板测定表明,TAFP不仅具有纤溶酶作用,还具有激活纤溶酶原的作用;通过三肽生色底物测定发现,TAFP能分解纤溶酶原激活剂的生色底物-Chromozym UK及S-2288.还能水解胰蛋白酶专-底物Bz-Phe-Val-Arg-NA及CBZ-Gly-Pro-Arg-NA,表明TAFP具有类胰蛋白酶活性,专-水解精氨酸形成的酰胺键(或肽键).TAFP无胰凝乳蛋白酶活性.

  13. Oceanographic profile data collected from CTD and sound velocimeter - moving vessel profiler casts aboard FA2805, FA2806, FA2808 and NOAA Ship FAIRWEATHER as part of project OPR-P183-FA-15 in the Gulf of Alaska and North Pacific Ocean from 2015-05-16 to 2015-06-14 (NCEI Accession 0130665)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0130665 includes physical and profile data collected aboard the FA2805, FA2806, FA2808 and NOAA Ship FAIRWEATHER during project OPR-P183-FA-15 in the...

  14. Medium Optimization for the Production of Fibrinolytic Enzyme by Paenibacillus sp. IND8 Using Response Surface Methodology

    Directory of Open Access Journals (Sweden)

    Ponnuswamy Vijayaraghavan

    2014-01-01

    Full Text Available Production of fibrinolytic enzyme by a newly isolated Paenibacillus sp. IND8 was optimized using wheat bran in solid state fermentation. A 25 full factorial design (first-order model was applied to elucidate the key factors as moisture, pH, sucrose, yeast extract, and sodium dihydrogen phosphate. Statistical analysis of the results has shown that moisture, sucrose, and sodium dihydrogen phosphate have the most significant effects on fibrinolytic enzymes production (P<0.05. Central composite design (CCD was used to determine the optimal concentrations of these three components and the experimental results were fitted with a second-order polynomial model at 95% level (P<0.05. Overall, 4.5-fold increase in fibrinolytic enzyme production was achieved in the optimized medium as compared with the unoptimized medium.

  15. Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells.

    Science.gov (United States)

    Lu, Sumei; Yu, Liang; Mu, Yakui; Ma, Juke; Tian, Jiajun; Xu, Wei; Wang, Haibo

    2014-07-01

    Multidrug resistance (MDR) is one of the most important obstacles affecting the efficacy of chemotherapy treatments for numerous types of cancer. In the present study, we have demonstrated the possible function of Twist1 in the chemosensitivity of head and neck squamous cell carcinoma (HNSCC) and have identified that its mechanism maybe associated with MDR1/P-gp regulation. To investigate this, the hypopharyngeal cancer cell line, FaDu, and its MDR cell line induced by taxol, FaDu/T, were employed. Stable transfectants targeted to Twist1 overexpression and Twist1 silencing based on FaDu were also conducted. Morphological observation, flow cytometry, reverse transcription-polymerase chain reaction (RT-PCR), western blotting and laser scanning confocal microscope detection were utilized to detect the associations between Twist1 and the chemosensitivity of FaDu cells. Our results demonstrated that Twist1 and MDR1/P-gp were upregulated in FaDu/T cells in a MDR dose-dependent manner. The anti-apoptotic capabilities of FaDu/T cells were enhanced during MDR progression, with apoptosis-related proteins (Bcl-2, Bax, activated caspase-3 and caspase-9) changing to resist apoptosis. Twist1 overexpression decreased the sensitivity of cells to taxol as revealed by a significant increase in MDR1/P-gp and IC50 (Pcell death, and inhibited Ca2+ release induced by taxol (Pcells also confirmed this result. This study provided evidence that alterations of Twist1 expression modulates the chemosensitivity of FaDu cells to taxol. Therefore, Twist1 knockdown may be a promising treatment regimen for advanced hypopharyngeal carcinoma patients with MDR.

  16. Procoagulant changes induced by oral contraceptives are balanced by an increased fibrinolytic tendency.

    Science.gov (United States)

    Mackie, I J; Campbell, S; Gallimore, M; Robinson, G; Machin, S J

    1992-01-01

    Oral contraceptives caused increased fibrinogen, FVII, FX, and fibrinolysis. The latter was associated with elevated FXII and PKK, while C1-INH was decreased, ATIII and alpha 2M were unchanged; it could not be accounted for by changes in t-PA, u-PA, PAI, plasminogen, alpha 2-AP, proteins C or S. HCII and alpha 1-PI were increased and may regulate the availability of thrombin and FXIa. The increased FXII/PKK dependent fibrinolytic potential and HCII may offset any increase in thrombin generation, while alpha 1-PI limits intrinsic coagulation.

  17. The role of FaBG3 in fruit ripening and B. cinerea fungal infection of strawberry.

    Science.gov (United States)

    Li, Qian; Ji, Kai; Sun, Yufei; Luo, Hao; Wang, Hongqing; Leng, Ping

    2013-10-01

    In plants, β-glucosidases (BG) have been implicated in developmental and pathogen defense, and are thought to take part in abscisic acid (ABA) synthesis via hydrolysis of ABA glucose ester to release active ABA; however, there is no genetic evidence for the role of BG genes in ripening and biotic/abiotic stress in fruits. To clarify the role of BG genes in fruit, eight Fa/FvBG genes encoding β-glucosidase were isolated using information from the GenBank strawberry nucleotide database. Of the Fa/FvBG genes examined, expression of FaBG3 was the highest, showing peaks at the mature stage, coincident with the changes observed in ABA content. To verify the role of this gene, we suppressed the expression of FaBG3 via inoculation with Agrobacterium tumefaciens containing tobacco rattle virus carrying a FaBG3 fragment (RNAi). The expression of FaBG3 in FaBG3-RNAi-treated fruit was markedly reduced, and the ABA content was lower than that of the control. FaBG3-RNAi-treated fruit did not exhibit full ripening, and were firmer, had lower sugar content, and were pale compared with the control due to down-regulation of ripening-related genes. FaBG3-RNAi-treated fruit with reduced ABA levels were much more resistant to Botrytis cinerea fungus but were more sensitive to dehydration stress than control fruit. These results indicate that FaBG3 may play key roles in fruit ripening, dehydration stress and B. cinerea fungal infection in strawberries via modulation of ABA homeostasis and transcriptional regulation of ripening-related genes.

  18. The biotechnological potential of subtilisin-like fibrinolytic enzyme from a newly isolated Lactobacillus plantarum KSK-II in blood destaining and antimicrobials.

    Science.gov (United States)

    Kotb, Essam

    2015-01-01

    An antimicrobial oxidative- and SDS-stable fibrinolytic alkaline protease designated as KSK-II was produced by Lactobacillus plantarum KSK-II isolated from kishk, a traditional Egyptian food. Maximum enzyme productivity was obtained in medium containing 1% lactose and 0.5% soybean flour as carbon and nitrogen sources, respectively. Purification of enzyme increased its specific activity to 1,140-fold with a recovery of 33% and molecular weight of 43.6 kDa. Enzyme activity was totally lost in the presence of ethylenediaminetetraacetic acid and was restored after addition of Fe(2+) suggesting that KSK-II is a metalloprotease and Fe(2+) acts as cofactor. Enzyme hydrolyzed not only the natural proteins but also synthetic substrates, particularly Suc-Ala-Ala-Pro-Phe-pNA. KSK-II can hydrolyze the Lys-X easier than Arg-X; thus, it was considered as a subtilisin-family protease. Its apparent Km , Vmax , and Kcat were 0.41 mM, 6.4 µmol mg(-1) min(-1) , and 28.0 s(-1) , respectively. KSK-II is industrially important from the perspectives of its maximal activity at 50°C (stable up to 70°C), ability to function at alkaline pH (10.0), stability at broad pH ranges (7.5-12.0) in addition to its stability toward SDS, H2 O2 , organic solvents, and detergents. We emphasize for the first time the potential of fibrinolytic activity for alkaline proteases used in detergents especially in blood destaining. © 2014 American Institute of Chemical Engineers.

  19. The effect of architectural façade design on energy savings in the student dormitory

    Directory of Open Access Journals (Sweden)

    Pejić Petar Č.

    2014-01-01

    Full Text Available There are many reasons for adequate use of natural light inside students' dormitories. Intensity of light required for student activities and temperature inside the rooms are the major factors for an occupant's comfortable work and life. Design of building façades has a significant impact both on the use of natural light and energy consumption. In this paper, a comparative analysis of student rooms with different orientations and different façade designs was performed in order to investigate what type of refurbishment in the façade is necessary. The goal of the refurbishment was generation of optimal thermal and lighting comfort for students' work with maximal energy saving for a new student dormitory in Nis, Serbia. An analysis of annual energy consumption of the newly designed student dormitory and proposed replacements on the exterior façade was performed by using the software EnergyPlus. Based on the energy consumption analysis it could be concluded that significant energy savings would be possible by upgrading the shading devices across the width of the window. In other words, changing the façade of the dorm could generally improve students' comfort, while the energy costs would be reduced. [Projekat Ministarstva nauke Republike Srbije, br. TR 36037: Development of student dorms in Serbia at the beginning of the 21st century i br. TR 33051: The concept of sustainable energy supply of settlements with energy efficient buildings

  20. International Façades - CROFT. Climate Related Optimized Façade Technologies

    Directory of Open Access Journals (Sweden)

    Marcel Bilow

    2012-04-01

    Full Text Available Looking at Central European building projects illustrates an awareness of sustainability and the need to save energy. This trend is based on the finiteness of natural resources, and is thus wise to follow. Developments in this region including passive house technologies, and energy plus solutions that create more energy than they use have become realisable. But it is not increasing technological knowledge alone that supported these developments; the Central European climate makes it possible to invent technological solutions that allow for maximum comfort while maintaining low energy consumption.Other regions have experienced a building boom over the past decades that has dramatically increased city sizes. A detailed examination of such building projects illustrates that most of them strive for the international standard with a high glazing ratio in the style of the Central European examples. But how can architecture be transferred to regions with entirely different climate conditions? The answer lies in the technological possibilities we have at our disposal today. The main research question of this thesis refers to utilising the local climate. Which methods are necessary to plan a building - and a façade as the interface between the inside and the outside, in particular - while working with, not against the climate? Sailing has been used as an analogy: only with the knowledge of winds and tides can we use them to efficiently move across bodies of water. Those who have not learned or understood this will have to use a motorboat and pay the price for petrol.Chapter 2 ‘Climate zones’ describes the different climate zones and their particularities, analysed with the help of eight different boomtowns. The mild Central European climate becomes particularly apparent when compared to tropic locations such as Singapore. Here, very high average temperatures and humidity levels require that we rethink and find new solutions.In chapter 3

  1. 木瓜中纤溶活性蛋白的分离与鉴定%Isolation and identification of a fibrinolytic protein in papaya

    Institute of Scientific and Technical Information of China (English)

    孟爱霞; 邱顺华; 钱民章

    2012-01-01

    从木瓜中分离出能溶解纤维蛋白的活性蛋白。木瓜新鲜乳汁经浸提、盐析、透析得蛋白粗提物;SephadexG-75凝胶层析分离蛋白粗提物,收集蛋白质的洗脱液,依序集合为共1~10个样品,纤维蛋白平板法筛选纤溶活性蛋白;SDS-PAGE电泳检测蛋白纯度及分子量;体外观察该蛋白对血凝块的溶解效果。木瓜蛋白粗提物有纤溶作用,经层析后得到的4、5、6号样品均有纤溶活性,5号样品SDS-PAGE电泳为单一蛋白条带,分子量为26ku,以尿激酶为标准标定该蛋白纤溶活性为17.85IU/mL。该蛋白在体外可通过激活纤溶酶原或直接降解纤维蛋白溶解血栓。木瓜中含有一种分子量为26ku的纤溶活性蛋白。%To isolate proteins from papaya with the ability to digest fibrin. Obtain the crude protein extracts from fresh papaya milk via the process of leaching, salting out and dialysis. Collect fractions No.1-10 as the results of submitting the protein extracts to a Sephadex G-75 chromatographic system and screen fibrinolytic proteins by the method of fibrin plate. Then test the molecule weight (MW) and purity of the protein by SDS-PAGE. The digesting effect of the protein on blood clots was observed in vitro. The crude protein extracts of papaya have the ability of fibrinolysis for which fra.4, 5 and 6 mainly account. And fra.5 appears to be single band in SDS-PAGE with the MW of 26 ku and has the fibrinolytic ability of 17.85 IU/ mL with urokinase as the standard. The protein could dissolve thrombus by either activating plasminogen in vitro or direct digestion of fibrin. We have found a fibrinolytic protein with the MW of 26 ku in papaya.

  2. Obesity is accompanied by disturbances in peripheral glucocorticoid metabolism and changes in FA recycling

    DEFF Research Database (Denmark)

    Simonyte, Kotryna; Rask, Eva; Näslund, Ingmar;

    2009-01-01

    The glucocorticoid activating enzyme 11beta-hydroxysteroid dehydrogenase type 1 (11betaHSD1) is of major interest in obesity-related morbidity. Alterations in tissue-specific cortisol levels may influence lipogenetic and gluco/glyceroneogenetic pathways in fat and liver. We analyzed the expressio...... acid (FA) recycling in adipose tissue (AT)....

  3. Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi

    NARCIS (Netherlands)

    Wang, C.T.; Ji, B.P.; Li, B.; Nout, M.J.R.; Li, P.L.; Ji, H.; Chen, L.F.

    2006-01-01

    Bacillus subtilis DC33 producing a novel fibrinolytic enzyme was isolated from Ba-bao Douchi, a traditional soybean-fermented food in China. The strong fibrin-specific enzyme subtilisin FS33 was purified to electrophoretic homogeneity using the combination of various chromatographic steps. The

  4. Mass spectrometry of the lithium adducts of diacylglycerols containing hydroxy FA in castor oil and two normal FA

    Science.gov (United States)

    Castor oil can be used in industry. The molecular species of triacylglycerols containing hydroxy fatty acids (FA) in castor oil have been identified. We report here the identification of twelve diacylglycerols (DAG) containing hydroxy FA in castor oil using positive ion electrospray ionization mass ...

  5. Combined treatment of ulinastatin and tranexamic acid provides beneficial effects by inhibiting inflammatory and fibrinolytic response in patients undergoing heart valve replacement surgery.

    Science.gov (United States)

    Chen, Ting-Ting; Jiandong-Liu; Wang, Gang; Jiang, Sheng-Li; Li, Li-Bing; Gao, Chang-Qing

    2013-02-01

    To investigate the effect of ulinastatin and tranexamic acid administered alone or in combination on inflammatory cytokines and fibrinolytic system in patients undergoing heart valve replacement surgery during cardiopulmonary bypass (CPB). CPB-induced fibrinolytic hyperfunction and systemic inflammatory response syndrome (SIRS) are the leading causes responsible for the occurrence of postsurgical complications such as postsurgical cardiac insufficiency and lung injury, which may lead to an increase in postsurgical bleeding, prolongation of hospital stay, and increased costs. One hundred twenty patients undergoing heart valve replacement surgery during CPB were randomly assigned into 4 groups of 30 patients each: blank control group (Group C), tranexamic acid group (Group T), ulinastatin group (Group U), and tranexamic acid-ulinastatin combination group (Group D). Physiological saline, tranexamic acid, ulinastatin, and a combination of tranexamic acid and ulinastatin were given to each group, respectively. Arterial blood was collected from the radial artery at 4 time points: after induction of anesthesia (T1), unclamping the ascending aorta (T2), and at 1 hour (T3) and 24 hours (T4) after CPB. The levels of plasma tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), neutrophil elastase (NE), and the concentrations of tissue plasminogen activator (t-PA) and α2-antiplasmin (α2-AP) were detected. The changes in the volume of pericardial mediastinal drainage after surgery were observed and recorded. The plasma TNF-α, IL-6, and NE levels significantly increased in patients from all 4 groups at time points of T2, T3, and T4 in comparison to those before CPB (P surgery (P surgery.

  6. Heart rate turbulence in patients after primary percutaneous coronary intervention and fibrinolytic treatment for acute myocardial infarction

    Directory of Open Access Journals (Sweden)

    Isman Firdaus

    2007-03-01

    Full Text Available Heart rate turbulence (HRT as novel predictor of sudden cardiac death were superior to all other presently available indicators. HRT significantly was improves after successful reperfusion reflecting rapid restoration of baroreceptor response. We investigated turbulence onset (TO and turbulence slope (TS values among patients with acute ST-elevation myocardial infarction (STEMI underwent revascularization by means of primary PCI or fibrinolytic. We hypothesized that the values of TO and TS were different in two kinds of revascularization treatment. The subjects underwent 24 hours ECG recording after revascularization therapy. TO was quantified by the relative change of the first two sinus RR intervals following a ventricular premature beat (VPB and the last two sinus RR intervals before the VPB. TS was quantified by the maximum positive slope of a regression line assessed over any sequence of five subsequent sinus rhythm RR intervals within the first two sinus rhythm intervals after a VPB. Thirteen patients (mean of age 56 ± 9 years old who underwent revascularization treatment of acute STEMI were eligible as subject of this study.Ten patients underwent fibrinolytic therapy and three patients underwent primary PCI. TO value was significantly different between PCI group and fibrinolytic group (-3.3 ± 1.7 % vs -0.2 ± 0.9 % ; P=0.03. The Primary PCI group has better outcome on turbulence slope value (TS than fibrinolytic group but not significance (7.7 ± 4.4 msec/RR interval vs 3.4 ± 2.6 msec/RR interval; P = 0.056. In conclusion, TO was better in acute STEMI patient undergone PCI compare to that undergone fibrinolytic therapy. (Med J Indones 2007; 16:19-24 Keywords: heart rate turbulence, revascularization, myocardial infarction

  7. Xanthohumol lowers body weight and fasting plasma glucose in obese male Zucker fa/fa rats.

    Science.gov (United States)

    Legette, Leecole L; Luna, Arlyn Y Moreno; Reed, Ralph L; Miranda, Cristobal L; Bobe, Gerd; Proteau, Rosita R; Stevens, Jan F

    2013-07-01

    Obesity contributes to increased risk for several chronic diseases including cardiovascular disease and type 2 diabetes. Xanthohumol, a prenylated flavonoid from hops (Humulus lupulus), was tested for efficacy on biomarkers of metabolic syndrome in 4 week old Zucker fa/fa rats, a rodent model of obesity. Rats received daily oral doses of xanthohumol at 0, 1.86, 5.64, and 16.9 mg/kg BW for 6 weeks. All rats were maintained on a high fat (60% kcal) AIN-93G diet for 3 weeks to induce severe obesity followed by a normal AIN-93G (15% kcal fat) diet for the last 3 weeks of the study. Weekly food intake and body weight were recorded. Plasma cholesterol, glucose, insulin, triglyceride, and monocyte chemoattractant protein-1 (MCP-1) levels were assessed using commercial assay kits. Plasma and liver tissue levels of XN and its metabolites were determined by liquid-chromatography tandem mass spectrometry. Plasma and liver tissue levels of xanthohumol were similar between low and medium dose groups and significantly (pxanthohumol, which was also not seen for female rats. Plasma cholesterol, insulin, triglycerides, and MCP-1 as well as food intake were not affected by treatment. The findings suggest that xanthohumol has beneficial effects on markers of metabolic syndrome.

  8. Epigenetics and development of food allergy (FA) in early childhood.

    Science.gov (United States)

    Hong, Xiumei; Wang, Xiaobin

    2014-09-01

    This review aims to highlight the latest advance on epigenetics in the development of food allergy (FA) and to offer future perspectives. FA, a condition caused by an immunoglobulin (Ig) E-mediated hypersensitivity reaction to food, has emerged as a major clinical and public health problem worldwide in light of its increasing prevalence, potential fatality, and significant medical and economic impact. Current evidence supports that epigenetic mechanisms are involved in immune regulation and that the epigenome may represent a key "missing piece" of the etiological puzzle for FA. There are a growing number of population-based epigenetic studies on allergy-related phenotypes, mostly focused on DNA methylation. Previous studies mostly applied candidate-gene approaches and have demonstrated that epigenetic marks are associated with multiple allergic diseases and/or with early-life exposures relevant to allergy development (such as early-life smoking exposure, air pollution, farming environment, and dietary fat). Rapid technological advancements have made unbiased genome-wide DNA methylation studies highly feasible, although there are substantial challenge in study design, data analyses, and interpretation of findings. In conclusion, epigenetics represents both an important knowledge gap and a promising research area for FA. Due to the early onset of FA, epigenetic studies of FA in prospective birth cohorts have the potential to better understand gene-environment interactions and underlying biological mechanisms in FA during critical developmental windows (preconception, in utero, and early childhood) and may lead to new paradigms in the diagnosis, prevention, and management of FA and provide novel targets for future drug discovery and therapies for FA.

  9. Role of p53-fibrinolytic system cross-talk in the regulation of quartz-induced lung injury.

    Science.gov (United States)

    Bhandary, Yashodhar P; Shetty, Shwetha K; Marudamuthu, Amarnath S; Fu, Jian; Pinson, Barbara M; Levin, Jeffrey; Shetty, Sreerama

    2015-03-01

    Silica is the major component of airborne dust generated by wind, manufacturing and/or demolition. Chronic occupational inhalation of silica dust containing crystalline quartz is by far the predominant form of silicosis in humans. Silicosis is a progressive lung disease that typically arises after a very long latency and is a major occupational concern with no known effective treatment. The mechanism of silicosis is not clearly understood. However, silicosis is associated with increased cell death, expression of redox enzymes and pro-fibrotic cytokines and chemokines. Since alveolar epithelial cell (AEC) death and disruption of alveolar fibrinolysis is often associated with both acute and chronic lung injuries, we explored whether p53-mediated changes in the urokinase-type plasminogen activator (uPA) system contributes to silica-induced lung injury. We further sought to determine whether caveolin-1 scaffolding domain peptide (CSP), which inhibits p53 expression, mitigates lung injury associated with exposure to silica. Lung tissues and AECs isolated from wild-type (WT) mice exposed to silica exhibit increased apoptosis, p53 and PAI-1, and suppression of uPA expression. Treatment of WT mice with CSP inhibits PAI-1, restores uPA expression and prevents AEC apoptosis by suppressing p53, which is otherwise induced in mice exposed to silica. The process involves CSP-mediated inhibition of serine-15 phosphorylation of p53 by inhibition of protein phosphatase 2A-C (PP2A-C) interaction with silica-induced caveolin-1 in AECs. These observations suggest that changes in the p53-uPA fibrinolytic system cross-talk contribute to lung injury caused by inhalation of silica dust containing crystalline quartz and is protected by CSP by targeting this pathway.

  10. Fluorescent studies on the binding-Ca 2+ in fibrinolytic principle separated from snake venom

    Science.gov (United States)

    Wang, Shouye; Liu, Qingliang; Chen, Zhenlong; Zhang, Yuhui

    2000-08-01

    By using equilibrium dialysis, atomic absorption spectrometry, fluorescence titration and determination of fluorescence lifetime, it can be determined that each fibrinolytic principle (FP) molecule contains one Ca 2+-binding site and one Ca 2+ ion, which can be substituted by a Tb 3+ ion completely. The intramolecular energy transfer between Tb 3+ and the tryptophan (Trp) residue in FP has been investigated through fluorescence spectroscopy. In the FP molecule, the excited energy can transfer from the Trp residue as an energy donor to the Tb 3+ ion substituted as an acceptor. The distance between Tb 3+ and the Trp residue, ˜0.38 nm, has been calculated with the experimental data and Forster theory.

  11. Fibrinolytic therapy for thrombosis in cardiac valvular prosthesis short and long term results

    Directory of Open Access Journals (Sweden)

    Auristela I. O. Ramos

    2003-10-01

    Full Text Available OBJECTIVE: To assess the short- and long-term results of the use of streptokinase (SK for the treatment of thromboses in cardiac valvular prostheses. METHODS: Seventeen patients with cardiac prosthetic thrombosis diagnosed by clinical, echocardiographic, and radioscopic findings underwent fibrinolytic treatment with a streptokinase bolus of 250,000 U followed by 100.000 U/hour. Short- and long-term results were assessed by radioscopy and echocardiography. RESULTS: Of the 17 patients, 12 had mechanical double-disk prostheses (4 aortic, 6 mitral, 2 tricuspid, 4 had single-disk prostheses (2 aortic, 1 mitral, and 1 tricuspid, and 1 had a tricuspid bioprosthesis. The success rate was 64.8%, the partial success rate was 17.6%, and the nonsuccess rate was 17.6%. All patients with a double-disk prosthesis responded, completely or partially, to the treatment. None of the patients with a single-disk prosthesis had complete resolution of the thrombosis. The time of streptokinase infusion ranged from 6 to 80 hours (mean of 56 h. The mortality rate due to the use of streptokinase was 5.8% and was secondary to cerebral bleeding. During streptokinase infusion, 3 (17.6% embolic episodes occurred as follows: 1 cerebral, 1 peripheral, and 1 coronary. The rethrombosis index was 33% in a mean follow-up of 42 months. CONCLUSION: The use of fibrinolytic agents was effective and relatively safe in patients with primary thrombosis of a double-disk prosthesis. A fatal hemorrhagic complication occurred in 1 (5.8% patient, and embolic complications occurred in 3 (17.6% patients. In a mean 42-month follow-up, 67% of the patients were free from rethrombosis.

  12. 龙蛭胶囊体内外对动物凝血、纤溶功能的影响%THE ANTICOAGULATION AND FIBRINOLYTIC EFFECTS OF CAPSULE OF LONGZHI IN VITRO AND VIVO

    Institute of Scientific and Technical Information of China (English)

    黎渊弘; 戴曦; 钟小斌

    2012-01-01

    Objective: To study the anticogagulation and fibrinolytic effects of Capsule of Longzhi in experimental animals. Methods; The activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT). Clotting time (CT), fibrinogen content were determined by kit illustrations; the blood-clot lysis time (BLT), euglobulinlysis test (ELT) were determined by routine method and fibrinolytic activity was determined by fibrin plate process. Results; The Capsule of Longzhi could obviously prolong APTT, PT, TT, CT to produce anticoagulant effect and would decrease fibrinogen and shorten BLT and ELT in vitro and in vivo ( P <0. 05 or <0. 01). It had indirectly fibrinolytic avtivity. Conclusion; The Capsule of Longzhi has obvious anticoagulation and fibrinolytic effect on animals.%目的:探讨龙蛭胶囊对动物体内外抗凝血、纤维蛋白溶解的作用.方法:按试剂盒说明对龙蛭胶囊进行活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)、纤维蛋白原(FIB)含量的测定.用常规方法测定体外凝血时间(CT)、血块溶解时间(BLT)、优球蛋白溶解时间(ELT).采用纤维蛋白平板法测定龙蛭胶囊纤溶活性.结果:龙蛭胶囊能明显延长动物APTT、PT、TT,降低FIB含量.动物的BLT、ELT与生理盐水对照组比较明显缩短,差异均有统计学意义(P<0.05或P<0.01).在普通纤维蛋白平板和加热平板上能溶解纤维蛋白.结论:龙蛭胶囊具有抗凝作用,能直接溶解纤维蛋白.

  13. Framework for benchmarking FA-based string recognizers

    CSIR Research Space (South Africa)

    Ngassam, EK

    2010-10-01

    Full Text Available Previous work on implementations of FA-based string recognizers suggested a range of implementation strategies (and therefore, algorithms) aiming at improving their performance for fast string recognition. However, an efficient exploitation...

  14. Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation

    Science.gov (United States)

    Rajendran, P.; Young Kwon, Oh; Kim, Young Ock

    2017-01-01

    Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO4 were the vital parameters with statistical significance (p white (100%), and bovine serum albumin (29 ± 4.9%). PMID:28321408

  15. Suppression of Human T Cell Proliferation Mediated by the Cathepsin B Inhibitor, z-FA-FMK Is Due to Oxidative Stress.

    Directory of Open Access Journals (Sweden)

    Tanuja Rajah

    Full Text Available The cathepsin B inhibitor, benzyloxycarbonyl-phenylalanine-alanine-fluoromethyl ketone (z-FA-FMK readily inhibits anti-CD3-induced human T cell proliferation, whereas the analogue benzyloxycarbonyl-phenylalanine-alanine-diazomethyl ketone (z-FA-DMK had no effect. In contrast, benzyloxycarbonyl-phenylalanine-alanine-chloromethyl ketone (z-FA-CMK was toxic. The inhibition of T cell proliferation mediated by z-FA-FMK requires not only the FMK moiety, but also the benzyloxycarbonyl group at the N-terminal, suggesting some degree of specificity in z-FA-FMK-induced inhibition of primary T cell proliferation. We showed that z-FA-FMK treatment leads to a decrease in intracellular glutathione (GSH with a concomitant increase in reactive oxygen species (ROS levels in activated T cells. The inhibition of anti-CD3-induced T cell proliferation mediated by z-FA-FMK was abolished by the presence of low molecular weight thiols such as GSH, N-acetylcysteine (NAC and L-cysteine, whereas D-cysteine which cannot be metabolised to GSH has no effect. The inhibition of anti-CD3-induced up-regulation of CD25 and CD69 expression mediated by z-FA-FMK was also attenuated in the presence of exogenous GSH. Similar to cell proliferation, GSH, NAC and L-cysteine but not D-cysteine, completely restored the processing of caspase-8 and caspase-3 to their respective subunits in z-FA-FMK-treated activated T cells. Our collective results demonstrated that the inhibition of T cell activation and proliferation mediated by z-FA-FMK is due to oxidative stress via the depletion of GSH.

  16. Fibrinolytic enzyme production by newly isolated Bacillus cereus SRM-001 with enhanced in-vitro blood clot lysis potential.

    Science.gov (United States)

    Narasimhan, Manoj Kumar; Chandrasekaran, Muthukumaran; Rajesh, Mathur

    2015-01-01

    The discovery of plasmin-like microbial fibrinolytic enzymes having high specificity and negligible side effects is crucial for thrombolytic therapy. Herein, we report one such extra-cellular fibrinolytic enzyme producing Bacillus cereus SRM-001 isolated from the blood-laden soil of a chicken dump yard. The potency of the enzyme was established with fibrin plate assay and in-vitro blood clot lysis assay. The shake-flask operating parameters and media composition were optimized for maximizing the productivity of the enzyme. The operating parameters, pH 7, 37°C, 1% inoculum volume and 24 h inoculum age, were found to be the optimum. The levels of media components, corn flour (0.3% w/v), soyabean powder (1.9% w/v) and MnSO4 (11.5 mM) were optimized by statistical analysis using Box-Behnken design derived RSM. This resulted in an almost 1.8 fold increase in fibrinolytic enzyme productivity. The 3D response surface plots showed soyabean powder and MnSO4 to be the key ingredients for enhancing the enzyme productivity, whereas corn flour had a marginal effect. The in-vitro blood clot lysis assay conducted at near physiological pH 7 at 37°C showed the enzyme to be a potential therapeutic thrombolytic agent.

  17. Inhibitory Effects of Verrucarin A on Tunicamycin-Induced ER Stress in FaO Rat Liver Cells.

    Science.gov (United States)

    Bae, Eun Young; Lee, Seung Woong; Seong, Sin; Cho, Wonjun; Ahn, Jong Seog; Cho, Hyun-Sug

    2015-05-19

    Endoplasmic reticulum (ER) stress is linked with development and maintenance of cancer, and serves as a therapeutic target for treatment of cancer. Verrucarin A, isolated from the broth of Fusarium sp. F060190, showed potential inhibitory activity on tunicamycin-induced ER stress in FaO rat liver cells. In addition, the compound decreased tunicamycin-induced GRP78 promoter activity in a dose dependent manner without inducing significant inhibition of luciferase activity and cell growth for 6 and 12 h. Moreover, the compound decreased the expression of GRP78, CHOP, XBP-1, and suppressed XBP-1, and reduced phosphorylation of IRE1α in FaO rat liver cells. This evidence suggests for the first time that verrucarin A inhibited tunicamycin-induced ER stress in FaO rat liver cells.

  18. Platelet factor XIII increases the fibrinolytic resistance of platelet-rich clots by accelerating the crosslinking of alpha 2-antiplasmin to fibrin

    Science.gov (United States)

    Reed, G. L.; Matsueda, G. R.; Haber, E.

    1992-01-01

    Platelet clots resist fibrinolysis by plasminogen activators. We hypothesized that platelet factor XIII may enhance the fibrinolytic resistance of platelet-rich clots by catalyzing the crosslinking of alpha 2-antiplasmin (alpha 2AP) to fibrin. Analysis of plasma clot structure by polyacrylamide gel electrophoresis and immunoblotting revealed accelerated alpha 2AP-fibrin crosslinking in platelet-rich compared with platelet-depleted plasma clots. A similar study of clots formed with purified fibrinogen (depleted of factor XIII activity), isolated platelets, and specific factor XIII inhibitors indicated that this accelerated crosslinking was due to the catalytic activity of platelet factor XIII. Moreover, when washed platelets were aggregated by thrombin, there was evidence of platelet factor XIII-mediated crosslinking between platelet alpha 2AP and platelet fibrin(ogen). Specific inhibition (by a monoclonal antibody) of the alpha 2AP associated with washed platelet aggregates accelerated the fibrinolysis of the platelet aggregate. Thus in platelet-rich plasma clots, and in thrombin-induced platelet aggregates, platelet factor XIII actively formed alpha 2AP-fibrin crosslinks, which appeared to enhance the resistance of platelet-rich clots to fibrinolysis.

  19. FaPYR1 is involved in strawberry fruit ripening.

    Science.gov (United States)

    Chai, Ye-Mao; Jia, Hai-Feng; Li, Chun-Li; Dong, Qing-Hua; Shen, Yuan-Yue

    2011-10-01

    Although the plant hormone abscisic acid (ABA) has been suggested to play a role in the ripening of non-climatic fruit, direct genetic/molecular evidence is lacking. In the present study, a strawberry gene homologous to the Arabidopsis ABA receptor gene PYR1, named FaPYR1, was isolated and characterized. The 627 bp cDNA includes an intact open reading frame that encodes a deduced protein of 208 amino acids, in which putative conserved domains were detected by homology analysis. Using tobacco rattle virus-induced gene silencing (VIGS), the FaPYR1 gene was silenced in strawberry fruit. Down-regulation of the FaPYR1 gene not only significantly delayed fruit ripening, but also markedly altered ABA content, ABA sensitivity, and a set of ABA-responsive gene transcripts, including ABI1 and SnRK2. Furthermore, the loss of red colouring in FaPYR1 RNAi (RNA interference) fruits could not be rescued by exogenously applied ABA, which could promote the ripening of wild-type fruits. Collectively, these results demonstrate that the putative ABA receptor FaPYR1 acts as a positive regulator in strawberry fruit ripening. It was also revealed that the application of the VIGS technique in strawberry fruit could be used as a novel tool for studying strawberry fruit development.

  20. La faïence de Nevers (1585-1900

    Directory of Open Access Journals (Sweden)

    Jean Rosen

    2010-10-01

    Full Text Available Depuis 1585, et sans solution de continuité jusqu’à nos jours, Nevers, aussi bien par son importance historique que par le nombre de ses manufactures – douze en 1755, plus de trente ateliers en tout –, a joué un rôle de tout premier plan dans la propagation et l’évolution de la faïence française, du xvie au xxe siècle, et peut être considéré comme le centre idéal pour une recherche sur le long terme. Si la faïence de Nevers a déjà été abordée de multiples façons dans divers colloques et exp...

  1. Changes in Coagulation and Fibrinolytic Indices in Women with Polycystic Ovarian Syndrome Undergoing Controlled Ovarian Hyperstimulation

    Directory of Open Access Journals (Sweden)

    Ying Huang

    2014-01-01

    Full Text Available Background. Polycystic ovarian syndrome (PCOS women undergoing in vitro fertilization and embryo transfer (IVF-ET treatment always attain a low cumulative pregnancy rate disaccording with the satisfactory number of oocytes. Objective. We aim to evaluate the status of coagulation and fibrinolytic system in PCOS patients undergoing controlled ovarian hyperstimulation (COH process. Method. Of the 97 women, 30 patients with PCOS composed the study group; 67 women of child-bearing age with normal endocrine function composed the control group. All participants underwent GnRH agonist standard long protocol, and plasma HCY, FVIII, FX, and D-dimer levels as well as hormone parameters were measured at day of full downregulation, hCG priming, and embryos transfer. Results. On day of full downregulation, FX levels were significantly higher in PCOS group (P<0.01. On hCG priming day, FX and estrogen levels in PCOS group were higher than in the control group and FVIII levels were significantly lower on day of embryos transfer whereas FX and E2 levels were significantly higher in PCOS group. Conclusion. Hypercoagulable state during peri-implantation phase would probably lead to poor microcirculation of endometrium and be one of the most important disadvantages of successful implantation and subsequent clinical pregnancy.

  2. Fibrinolytic efficacy of Amediplase, Tenecteplase and scu-PA in different external plasma clot lysis models: sensitivity to the inhibitory action of thrombin activatable fibrinolysis inhibitor (TAFI).

    Science.gov (United States)

    Guimarães, Ana H C; Barrett-Bergshoeff, Marrie M; Criscuoli, Marco; Evangelista, Stefano; Rijken, Dingeman C

    2006-09-01

    In this study, the in-vitro fibrinolytic efficacy of Tenecteplase, Amediplase and scu-PA was investigated in different external lysis models by measuring the lysis of human plasma clots after the addition of the plasminogen activators (PAs) to the surrounding plasma. The effect of TAFI was examined for each PA by neutralising TAFIa with potato carboxypeptidase inhibitor (PCI). The lytic efficacy of Amediplase was lower than that of Tenecteplase at low PA concentrations but slightly higher at therapeutic concentrations. The activity of scu-PA was clearly lower than that of either Tenecteplase or Amediplase. The TAFI system inhibited external clot lysis mediated by all the PAs when thrombomodulin was present in the model. In the therapeutic range (5-10 mug/ml) however, the TAFIa effect was negligible for both Amediplase and Tenecteplase. At lower PA concentrations the effect of TAFI on Amediplase was slightly stronger than that on Tenecteplase. Under static conditions the lysis rates were lower than with stirring. The role of TAFI was similar under both conditions. In conclusion, at therapeutic concentrations Amediplase was slightly more active than Tenecteplase and scu-PA under all conditions used. Therefore, Amediplase might possibly be a more potent thrombolytic agent at these concentrations and increase the efficacy of thrombolysis. The potential of TAFI for inhibiting thrombolytic therapy is probably low. However in conditions where the local PA concentrations are sub-optimal TAFI might affect the lysis rate.

  3. 基于STATA的FA-DEA与PCA-DEA%Implementing FA-DEA and PCA-DEA in STATA

    Institute of Scientific and Technical Information of China (English)

    彭江平

    2015-01-01

    针对应用FA-DEA与PCA-DEA模型时,一般都需要同时使用统计分析软件与数据包络分析软件,提出了在STATA中的实现过程与方法,并进行了案例分析。方便了在实际应用过程中使用FA-DEA与PCA-DEA的过程,并为在统一STATA的环境下设计与应用新的数据包络法提供解决思路。%In general, the dimension reduction methods, such as FA and PCA, are implemented in statistical analysis software, but the data envelopment analysis is doing by DEA software. Simultaneously implementing FA-DEA and PCA-DEA all in STATA is puts forward, and an example is also listed. This approach will facilitate the process using FA-DEA and PCA-DEA in the practical application, and will provided a new solution in the STATA to design and application of data envelopment analysis.

  4. Late results of catheter-directed recombinant tissue plasminogen activator fibrinolytic therapy of iliofemoral deep venous thrombosis Resultados de longo prazo do tratamento fibrinolítico da trombose venosa iliacofemoral por infusão seletiva do ativador de plasminogênio tissular recombinante em baixas doses

    Directory of Open Access Journals (Sweden)

    Ivan Benaduce Casella

    2007-02-01

    Full Text Available PURPOSE: To evaluate the efficacy of catheter-directed low-dose recombinant tissue-type plasminogen activator infusion in the treatment of iliofemoral deep venous thrombosis and prevention of post-thrombotic syndrome. METHOD: Eighteen patients (out of 260 evaluated with acute iliofemoral deep venous thrombosis and no previous evidence of venous insufficiency were prospectively selected for thrombolytic therapy. Catheter-directed low-dose recombinant tissue-type plasminogen activator (1 mg/h was infused into the thrombotic segments. RESULTS: Effective fibrinolysis was achieved in 14 of 18 cases, with correlation between effective fibrinolysis and major/complete resolution of acute signs and symptoms (P OBJETIVOS: Avaliar a eficácia da infusão seletiva por cateter do ativador de plasminogênio tecidual recombinante em baixas doses no tratamento da trombose venosa iliacofemoral e na prevenção da síndrome pós-trombótica. MÉTODO: Dezoito pacientes (de 260 avaliados portadores de trombose venosa profunda iliacofemoral sem evidência prévia de insuficiência venosa foram selecionados para terapia fibrinolítica e submetidos a infusão seletiva por cateter do ativador de plasminogênio tecidual recombinante na dose de 1mg/dl nos segmentos venosos trombóticos. RESULTADOS: Quatorze pacientes apresentaram fibrinólise efetiva; observamos correlação entre o grau de melhora clínica observado e a redução percentual do volume trombótico (P<.01. Não houve episódios de complicações graves. Quatro pacientes apresentaram retrombose precoce (1 a 8 semanas. Os pacientes foram seguidos por um período de até 131 semanas (média 85.2. A incidência de sinais e sintomas clínicos de insuficiência venosa e os achados ecográficos de refluxo valvular foram significativamente menores nos pacientes em que a terapia fibrinolítica foi efetiva e a perviedade mantida ao longo do período de seguimento, na comparação com os casos de falha aguda ou de

  5. FaSa: A Fast and Stable Quadratic Placement Algorithm

    Institute of Scientific and Technical Information of China (English)

    HOU WenTing(侯文婷); HONG XianLong(洪先龙); WU WeiMin(吴为民); CAI YiCi(蔡懿慈)

    2003-01-01

    Placement is a critical step in VLSI design because it dominates overall speed andquality of design flow. In this paper, a new fast and stable placement algorithm called FaSa is pro-posed. It uses quadratic programming model and Lagrange multiplier method to solve placementproblems. And an incremental LU factorization method is used to solve equations for speeding up.The experimental results show that FaSa is very stable, much faster than previous algorithms andits total wire length is comparable with other algorithms.

  6. Role of p53–fibrinolytic system cross-talk in the regulation of quartz-induced lung injury

    Energy Technology Data Exchange (ETDEWEB)

    Bhandary, Yashodhar P.; Shetty, Shwetha K.; Marudamuthu, Amarnath S. [Texas Lung Injury Institute, Department of Medicine, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708 (United States); Fu, Jian [Texas Lung Injury Institute, Department of Medicine, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708 (United States); Center for Research on Environmental Disease and Toxicology, College of Medicine, University of Kentucky, Lexington, KY 40536 (United States); Pinson, Barbara M. [Occupational Medicine, Department of Medicine, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708 (United States); Center for Research on Environmental Disease and Toxicology, College of Medicine, University of Kentucky, Lexington, KY 40536 (United States); Levin, Jeffrey [Occupational Medicine, Department of Medicine, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708 (United States); Shetty, Sreerama, E-mail: sreerama.shetty@uthct.edu [Texas Lung Injury Institute, Department of Medicine, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708 (United States)

    2015-03-01

    Silica is the major component of airborne dust generated by wind, manufacturing and/or demolition. Chronic occupational inhalation of silica dust containing crystalline quartz is by far the predominant form of silicosis in humans. Silicosis is a progressive lung disease that typically arises after a very long latency and is a major occupational concern with no known effective treatment. The mechanism of silicosis is not clearly understood. However, silicosis is associated with increased cell death, expression of redox enzymes and pro-fibrotic cytokines and chemokines. Since alveolar epithelial cell (AEC) death and disruption of alveolar fibrinolysis is often associated with both acute and chronic lung injuries, we explored whether p53-mediated changes in the urokinase-type plasminogen activator (uPA) system contributes to silica-induced lung injury. We further sought to determine whether caveolin-1 scaffolding domain peptide (CSP), which inhibits p53 expression, mitigates lung injury associated with exposure to silica. Lung tissues and AECs isolated from wild-type (WT) mice exposed to silica exhibit increased apoptosis, p53 and PAI-1, and suppression of uPA expression. Treatment of WT mice with CSP inhibits PAI-1, restores uPA expression and prevents AEC apoptosis by suppressing p53, which is otherwise induced in mice exposed to silica. The process involves CSP-mediated inhibition of serine-15 phosphorylation of p53 by inhibition of protein phosphatase 2A-C (PP2A-C) interaction with silica-induced caveolin-1 in AECs. These observations suggest that changes in the p53–uPA fibrinolytic system cross-talk contribute to lung injury caused by inhalation of silica dust containing crystalline quartz and is protected by CSP by targeting this pathway. - Highlights: • Chronic exposure to quartz dusts is a major cause of lung injury and silicosis. • The survival of patients with silicosis is bleak due to lack of effective treatments. • This study defines a new role of

  7. Efficient Thermal Insulation of Passive House with Curved Façades in Cold Climate

    Directory of Open Access Journals (Sweden)

    Aznabaev Askar

    2016-01-01

    Full Text Available In this paper there is exploration of the possibility of creating effective sealed building envelope in zero energy building (ZEB with unique façades. This article describes passive and active measures of decrease of thermal loss through enclosure structures, usage of modern insulation materials; positive effect of rationalization of the façade orientation and form of a building in a plan; on the base of existing architecture concept structural solutions of building envelope are suggested. Using thermal FEA the enclosure structure was optimized. The results of modeling of units and elements of building envelope are: heat transfer coefficient U-value for heterogeneous multilayer structure, picture of temperature distribution and magnitude of the thermal conductivity and temperature of internal wall surface.

  8. High-intensity running in English FA Premier League soccer matches

    DEFF Research Database (Denmark)

    Bradley, Paul S.; Sheldon, William; Wooster, Blake

    2009-01-01

    The aims of this study were to (1) determine the activity profiles of a large sample of English FA Premier League soccer players and (2) examine high-intensity running during elite-standard soccer matches for players in various playing positions. Twenty-eight English FA Premier League games were.......01), attackers (2341 m, s=575, P game, high-intensity running distance was approximately 20% less than in the first 15-min period for wide midfielders (467 m, s=104 vs. 589 m, s=134, P ....01) and without ball possession (229 m, s=85 vs. 278 m, s=97, P game. Mean recovery time between very high-intensity running bouts was 72 s (s=28), with a 28% longer recovery time during the last 15 min than the first 15 min of the game (83 s, s=26 vs...

  9. Formal characterizations of FA-based string processors

    CSIR Research Space (South Africa)

    Ngassam, EK

    2010-08-01

    Full Text Available With this poster, the authors on the denotational semantics of algorithms to suggest an abstraction of a string recognizer. The abstraction provides a unified formalism for representing FA-based string recognizers as an instance of a parameterized...

  10. "What D'ya Mean, Project SOL-FA?"

    Science.gov (United States)

    Hardman, Olga S.

    1981-01-01

    Since 1977, Project Sol-fa, funded with an ESEA Title IV-C grant has provided Harrison County primary teachers with inservice training in the Kodaly method of music education. This article provides information on program funding, costs, and accomplishments. Evaluation forms and the inservice syllabus are appended. (SJL)

  11. Development and characterization of a high sensitivity segmented Fast Neutron Spectrometer (FaNS-2)

    Science.gov (United States)

    Langford, T. J.; Beise, E. J.; Breuer, H.; Heimbach, C. R.; Ji, G.; Nico, J. S.

    2016-01-01

    We present the development of a segmented fast neutron spectrometer (FaNS-2) based upon plastic scintillator and 3He proportional counters. It was designed to measure both the flux and spectrum of fast neutrons in the energy range of few MeV to 1 GeV. FaNS-2 utilizes capture-gated spectroscopy to identify neutron events and reject backgrounds. Neutrons deposit energy in the plastic scintillator before capturing on a 3He nucleus in the proportional counters. Segmentation improves neutron energy reconstruction while the large volume of scintillator increases sensitivity to low neutron fluxes. A main goal of its design is to study comparatively low neutron fluxes, such as cosmogenic neutrons at the Earth's surface, in an underground environment, or from low-activity neutron sources. In this paper, we present details of its design and construction as well as its characterization with a calibrated 252Cf source and monoenergetic neutron fields of 2.5 MeV and 14 MeV. Detected monoenergetic neutron spectra are unfolded using a Singular Value Decomposition method, demonstrating a 5% energy resolution at 14 MeV. Finally, we discuss plans for measuring the surface and underground cosmogenic neutron spectra with FaNS-2.

  12. [Data mining analysis of professor Li Fa-zhi AIDS herpes zoster medical record].

    Science.gov (United States)

    Wang, Dan-Ni; Li, Zhen; Xu, Li-Ran; Guo, Hui-Jun

    2013-08-01

    Analysis of professor Li Fa-zhi in the treatment of AIDS drug laws of herpes zoster and postherpetic neuralgia, provide reference for the use of Chinese medicine treatment of AIDS, herpes zoster and postherpetic neuralgia. By using the method of analyzing the complex network of Weishi county, Henan in 2007 October to 2011 July during an interview with professor Li Fa-zhi treatment of AIDS of herpes zoster and postherpetic neuralgia patients, patients are input structured clinical information collection system, into the analysis of the data, carries on the research analysis theory of traditional Chinese medicine compatibility system algorithm and complex network analysis the use of complex networks. The use of multi-dimensional query analysis of AIDS drugs, the core of herpes zoster and postherpetic neuralgia treated in this study are Scutellariae Radix, Glucyrrhizae Radix, Carthame Flos, Plantaginis Semen, Trichosamthis Fructus, Angelicae Sinensis Radix, Gentianae Radix; core prescription for Longdan Xiegan decoction and Trichosanthes red liquorice decoction. Professor Li Fa-zhi treatment of AIDS, herpes zoster and postherpetic neuralgia by clearing heat and removing dampness and activating blood circulation to.

  13. Metformin ameliorates diabetes but does not normalize the decreased GLUT 4 content in skeletal muscle of obese (fa/fa) Zucker rats

    DEFF Research Database (Denmark)

    Handberg, A; Kayser, L; Høyer, P E

    1993-01-01

    We studied the expression of the glucose transporter GLUT 4 in the soleus and red gastrocnemius muscles from obese, diabetic (fa/fa) Zucker rats compared to their lean littermates (Fa/-), with and without treatment with the antidiabetic drug metformin. In the untreated groups of rats, the GLUT 4...... content in a crude membrane fraction of both the soleus and the red gastrocnemius muscles were significantly lower in the obese (fa/fa) rats (3.46 +/- 0.28 vs. 6.04 +/- 0.41, p GLUT 4 expression in soleus muscle from...... the same rats were confirmed by quantitative immunofluorescence microscopy, and the results were significantly correlated with the results obtained from quantitative immunoblotting (rho = 0.70, p GLUT 4 in fa/fa rats could contribute to the well-established insulin...

  14. The strawberry transcription factor FaMYB1 inhibits the biosynthesis of proanthocyanidins in Lotus corniculatus leaves.

    Science.gov (United States)

    Paolocci, Francesco; Robbins, Mark P; Passeri, Valentina; Hauck, Barbara; Morris, Phil; Rubini, Andrea; Arcioni, Sergio; Damiani, Francesco

    2011-01-01

    Proanthocyanidins (PAs) are agronomically important biopolymers in higher plants composed primarily of catechin and epicatechin units. The biosynthesis of these natural products is regulated by transcription factors including proteins of the R2R3MYB class. To gain insight into the genetic control of the catechin and epicatechin branches of the PA pathway in forage legumes, here the effects of the expression of FaMYB1, a flavonoid R2R3MYB repressor from strawberry, in Lotus corniculatus (birdsfoot trefoil), were tested. It was found that in leaves of T(0) transgenic lines the degree of PA inhibition correlated with the level of FaMYB1 expression. These effects were heritable in the transgene-positive plant T(1) generation and were tissue specific as the suppression of proanthocyanidin biosynthesis was most pronounced in mesophyll cells within the leaf, whereas other flavonoid and phenolic compounds were substantially unaltered. The data suggest that FaMYB1 may counter-balance the activity of the endogenous transcriptional MYB-bHLH-WD40 (MBW) complex promoting proanthocyanidin biosynthesis via the catechin and epicatechin branches and that FaMYB1 does not interfere with the expression levels of a resident R2R3MYB activator of PAs. It is proposed that in forage legumes leaf cell commitment to synthesize proanthocyanidins relies on the balance between the activity of activator and repressor MYBs operating within the MBW complex.

  15. Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression.

    Science.gov (United States)

    Jakka, Siva R K; Gong, Liang; Hasler, James; Banerjee, Rahul; Sheets, Joel J; Narva, Kenneth; Blanco, Carlos A; Jurat-Fuentes, Juan L

    2015-12-04

    Insecticidal protein genes from the bacterium Bacillus thuringiensis (Bt) are expressed by transgenic Bt crops (Bt crops) for effective and environmentally safe pest control. The development of resistance to these insecticidal proteins is considered the most serious threat to the sustainability of Bt crops. Resistance in fall armyworm (Spodoptera frugiperda) populations from Puerto Rico to transgenic corn producing the Cry1Fa insecticidal protein resulted, for the first time in the United States, in practical resistance, and Bt corn was withdrawn from the local market. In this study, we used a field-collected Cry1Fa corn-resistant strain (456) of S. frugiperda to identify the mechanism responsible for field-evolved resistance. Binding assays detected reduced Cry1Fa, Cry1Ab, and Cry1Ac but not Cry1Ca toxin binding to midgut brush border membrane vesicles (BBMV) from the larvae of strain 456 compared to that from the larvae of a susceptible (Ben) strain. This binding phenotype is descriptive of the mode 1 type of resistance to Bt toxins. A comparison of the transcript levels for putative Cry1 toxin receptor genes identified a significant downregulation (>90%) of a membrane-bound alkaline phosphatase (ALP), which translated to reduced ALP protein levels and a 75% reduction in ALP activity in BBMV from 456 compared to that of Ben larvae. We cloned and heterologously expressed this ALP from susceptible S. frugiperda larvae and demonstrated that it specifically binds with Cry1Fa toxin. This study provides a thorough mechanistic description of field-evolved resistance to a transgenic Bt crop and supports an association between resistance and reduced Cry1Fa toxin binding and levels of a putative Cry1Fa toxin receptor, ALP, in the midguts of S. frugiperda larvae.

  16. Iodixanol Has a Favourable Fibrinolytic Profile Compared to Iohexol in Cardiac Patients Undergoing Elective Angiography: A Double-Blind, Randomized, Parallel Group Study.

    Directory of Open Access Journals (Sweden)

    Andrew T Treweeke

    Full Text Available There is no consensus and a limited evidence base for choice of contrast agents (CA in angiography. This study evaluated the impact of iohexol and iodixanol CA on fibrinolytic factors (tissue plasminogen activator [t-PA] and plasminogen activator inhibitor-1 [PAI-1], as well as platelet-monocyte conjugates in cardiac patients undergoing elective angiography in a double-blind, randomised parallel group study.Patients (men, 50-70 years old; n = 12 were randomised to receive either iohexol (Omnipaque; n = 6 or iodixanol (Visipaque; n = 6 during elective angiography at Raigmore Hospital, Inverness, UK. Arterial and venous blood samples were drawn prior to CA delivery and following angiography. Assessment of platelet-monocyte conjugation, t-PA and PAI-1 antigen and activity was conducted in samples pre- and post-angiography.Plasma t-PA antigen was depressed equally in the study groups after angiography, but there was a greater reduction in PAI-1 antigen in the group receiving iodixanol. These findings corresponded to a substantial reduction in t-PA activity in patients receiving iohexol, with no change in those receiving iodixanol (P = 0.023 between the CA groups. Both CAs caused a reduction in platelet-monocyte conjugation, with no difference between the groups. No adverse events were reported during the trial.Avoiding reduced plasma t-PA activity might be an important consideration in choosing iodixanol over iohexol in patients at risk of thrombosis following angiography. The trial is registered on the ISRCTN register (ISRCTN51509735 and funded by the Coronary Thrombosis Trust and National Health Service (Highland R&D Endowments. The funders had no influence over study design or reporting.Controlled-Trials.com ISRCTN51509735.

  17. Penerapan Perencanaan Pajak Penghasilan pada Fa Trico Paint Factory

    Directory of Open Access Journals (Sweden)

    Stefanus Ariyanto

    2011-05-01

    Full Text Available The most important parts of government income comes from taxes, especially income taxes. The different point of view arises between government and tax payer. While the government try to increase the tax income, the taxpayers always intend to minimize their tax burden by implementing tax management/plannning. This paper is a case study in FA Trico Paint Factory (FA TPF that try to reperforming company’s income tax return preparation with the main purpose to minimize company tax burden, while it is still comply with tax regulations in Indonesia. This approach could be an alternative for the company to restate it’s annual income tax return, to avoic fines and charges for not comply with the regulations, and minimize it’s income tax expense by approximately 10% per year. 

  18. Odenplan: a media façade design process

    DEFF Research Database (Denmark)

    Korsgaard, Henrik; Hansen, Nicolai Brodersen; Basballe, Ditte Amund;

    2012-01-01

    of how to design for media façades by discussing how we have structured our design process to address specific sets of challenges outlined in previous literature in the field of media architecture. In our view, such research is valuable in that it helps establish common ground for researchers...... and practitioners in a developing field by building a repertoire of approaches, as well as highlight important issues that need to be addressed in this emergent field....

  19. Optimizing Maintenance Manpower for USMC F/A-18 Squadrons

    Science.gov (United States)

    2016-06-01

    for Logistics Analysis and Technical Evaluation EMT Elapsed Maintenance Time F/A Fighter/Attack FAP Fleet Assistance Program FCF Functional Check... Occupational Specialty MVP Manpower Validation Planner NAVAIR Naval Air Systems Command NMC Non-Mission Capable NMCS Non-Mission Capable Supply O...the required rank and Military Occupational Specialty (MOS) for a squadron consisting of 23 officers and 167 enlisted Marines and a VMFA(AW) squadron

  20. Immunological characterization of plasminogen activator activities in human tissues and body fluids

    NARCIS (Netherlands)

    Rijken, D.C.; Wijngaards, G.; Welbergen, J.

    1981-01-01

    Human plasminogen activators were compared immunologically in both a double-diffusion technique and quenching experiments on the fibrinolytic activities of the activators. Antisera against HMW and LMW urokinase and an antiserum against highly purified tissue plasminogen activator from human uterus

  1. Effect of FA and GA on Germination of Winter Wheat%黄腐酸和赤霉素对冬小麦种子萌发的影响

    Institute of Scientific and Technical Information of China (English)

    陈玉玲; 李建华

    2001-01-01

    小麦种子萌发过程中,质膜相对透性、α-淀粉酶和过氧化物酶(POD)活性均呈现先上升后下降的变化趋势.FA、GA及二者混合处理均能促进小麦生长,提高α-淀粉酶活性,降低质膜相对透性,FA及FA+GA还能使POD活性增加.FA、FA+GA在小麦种子萌发过程中都具有GA的类似效果.%During the germination of wheat seeds, their membrane permeability, activities of α-amylase and peroxidase increased at first,then decreased. After treatment of FA,GA or FA+GA,the growth of seedling and the activity of α-amylase increased,membrane permeability decreased;the activity of POD decreased after treatment of FA and FA+GA. So we concluded FA and FA+GA had GA's function during germination.

  2. Differential modulation of the functionality of white adipose tissue of obese Zucker (fa/fa) rats by the type of protein and the amount and type of fat.

    Science.gov (United States)

    Díaz-Villaseñor, Andrea; Granados, Omar; González-Palacios, Berenice; Tovar-Palacio, Claudia; Torre-Villalvazo, Ivan; Olivares-García, Verónica; Torres, Nimbe; Tovar, Armando R

    2013-11-01

    Recent evidence indicates that several metabolic abnormalities developed during obesity are associated with the presence of dysfunctional adipose tissue. Diet is a key factor that modulates several functions of adipose tissue; however, each nutrient in the diet produces specific changes. Thus, the aim of this work was to study the effect of the interaction of the type (coconut or soybean oil) and amount (5% or 10%) of fat with the type of dietary protein (casein or soy protein) on the functionality of white adipose tissue of Zucker (fa/fa) rats. The results showed that soybean oil reduced adipocyte size and decreased esterified saturated fatty acids in white adipose tissue. Excess dietary fat also modified the composition of esterified fatty acids in white adipose tissue, increased the secretion of saturated fatty acids to serum from white adipose tissue and reduced the process of fatty acids re-esterification. On the other hand, soy protein sensitized the activation of the hormone-sensitive lipase by increasing the phosphorylation of this enzyme (Ser 563) despite rats fed soy protein were normoglucagonemic, in contrast with rats fed casein that showed hyperglucagonemia but reduced hormone-sensitive lipase phosphorylation. Finally, in white adipose tissue, the interaction between the tested dietary components modulated the transcription/translation process of lipid and carbohydrate metabolism genes via the activity of the PERK-endoplasmic reticulum stress response. Therefore, our results showed that the type of protein and the type and amount of dietary fat selectively modify the activity of white adipose tissue, even in a genetic model of obesity. Copyright © 2013 Elsevier Inc. All rights reserved.

  3. Lessons learned from the TIMI trials in rescue interventions for elderly patients after failed fibrinolytic therapy: look beyond the TIMI flow

    Institute of Scientific and Technical Information of China (English)

    Dayi HU; Yifang GUO; Thach Nguyen

    2005-01-01

    @@ In ST-segment elevation myocardial infarction (STEMI), acute reperfusion of the infarct-related artery (IRA)is the main goal in the early minutes after the patient seeks medical attention. Fibrinolytic therapy (FT) and/or primary coronary intervention (PCI) were proven to be effective in opening the IRA.

  4. Evidence for a positive regulatory role of strawberry (Fragaria x ananassa) Fa WRKY1 and Arabidopsis At WRKY75 proteins in resistance.

    Science.gov (United States)

    Encinas-Villarejo, Sonia; Maldonado, Ana M; Amil-Ruiz, Francisco; de los Santos, Berta; Romero, Fernando; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L

    2009-01-01

    Knowledge of the molecular basis of plant resistance to pathogens in species other than Arabidopsis is limited. The function of Fa WRKY1, the first WRKY gene isolated from strawberry (Fragaria x ananassa), an important agronomical fruit crop, has been investigated here. Fa WRKY1 encodes a IIc WRKY transcription factor and is up-regulated in strawberry following Colletotrichum acutatum infection, treatments with elicitors, and wounding. Its Arabidopsis sequence homologue, At WRKY75, has been described as playing a role in regulating phosphate starvation responses. However, using T-DNA insertion mutants, a role for the At WRKY75 and Fa WRKY1 in the activation of basal and R-mediated resistance in Arabidopsis is demonstrated. At wrky75 mutants are more susceptible to virulent and avirulent isolates of Pseudomonas syringae. Overexpression of Fa WRKY1 in At wrky75 mutant and wild type reverts the enhanced susceptible phenotype of the mutant, and even increases resistance to avirulent strains of P. syringae. The resistance phenotype is uncoupled to PATHOGENESIS-RELATED (PR) gene expression, but it is associated with a strong oxidative burst and glutathione-S-transferase (GST) induction. Taken together, these results indicate that At WRKY75 and Fa WRKY1 act as positive regulators of defence during compatible and incompatible interactions in Arabidopsis and, very likely, Fa WRKY1 is an important element mediating defence responses to C. acutatum in strawberry. Moreover, these results provide evidence that Arabidopsis can be a useful model for functional studies in Rosacea species like strawberry.

  5. FANCM-FAAP24 and FANCJ: FA proteins that metabolize DNA

    OpenAIRE

    Ali, Abdullah Mahmood; Singh, Thiyam Ramsing; Meetei, Amom Ruhikanta

    2009-01-01

    Fanconi anemia (FA) is a rare autosomal recessive or X-linked disorder characterized by aplastic anemia, cancer susceptibility and cellular sensitivity to DNA crosslinking agents. Eight FA proteins (FANCA, -B, -C, -E, -F, -G, -L and –M) and three non-FA proteins (FAAP100, FAAP24 and HES1) form the FA nuclear core complex that is required for monoubiquitination of the FANCD2-FANCI dimer upon DNA damage. The other three FA proteins, FANCD1/BRCA2, FANCJ/BACH1/BRIP1 and FANCN/PALB2, act in parall...

  6. Development of a Holistic Evaluation System for BIPV Façades

    Directory of Open Access Journals (Sweden)

    Thorsten Schuetze

    2015-06-01

    Full Text Available Façades with building-integrated photovoltaics (BIPV have the advantage that they can produce renewable electric energy. Compared with conventional façades, BIPV façades have therefore a valuable additional property that can generally contribute to increasing the degree of sustainability of buildings. A holistic assessment system for BIPV façade systems for office and administration buildings was developed in the framework of the project “MULTIELEMENT II” at the Fraunhofer Institute for Wind Energy and Energy System Technology (IWES in Kassel, Germany. The aim of this research was a comparison of conventional façade systems with BIPV façade systems regarding different technical, economical, sustainability, and design criteria. This paper discusses the basic conditions for a holistic evaluation of BIPV façades in comparison with conventional façades. A method for the execution of a holistic evaluation and characteristic values for a comparison interpretation of results is presented. Façade systems are evaluated regarding both quantifiable and non-quantifiable properties by means of a Microsoft Excel-based evaluation tool. The tool facilitates the comparison and evaluation of planned or built façades with and without BIPV. The detailed evaluation results aim to facilitate the certification of BIPV façade systems in the framework of sustainable building certification systems such as the German DGNB.

  7. Composting of food waste subjected to hydrothermal pretreatment and inoculated with Paecilomyces sp. FA13.

    Science.gov (United States)

    Nakasaki, Kiyohiko; Mimoto, Hiroshi; Tran, Quyen Ngoc Minh; Oinuma, Akiko

    2015-03-01

    Food waste collected from restaurants, convenience stores, and food-processing factories was mixed with sawdust and subjected to hydrothermal pretreatment at 180°C for 30min to prepare compost raw material. Furan compounds such as 5-HMF (5-hydroxymethyl furfural) and furfural were produced at concentration levels of approximately 8 and 0.5mg/g-ds, respectively, through hydrothermal pretreatment. The furan compounds inhibited the activity of composting microorganisms, thus delaying the start of organic matter degradation during composting. A newly identified fungus, Paecilomyces sp. FA13, which possesses the ability to degrade furan compounds, was isolated and used as an inoculum for the composting of the raw material prepared by hydrothermal pretreatment. By inoculating the FA13 into the compost raw material at 10(5)CFU/g-ds, the degradation of furan compounds was accelerated. As a result, bacterial activity, which contributed to composting, was enhanced, significantly promoting the start of vigorous degradation of organic materials.

  8. Façade fire tests – measurements and modeling

    Directory of Open Access Journals (Sweden)

    Anderson Johan

    2013-11-01

    Full Text Available In two recent papers [1, 2] the fire dynamics in a test rig for façade constructions according to the test method SP Brand 105 [3, 4] was investigated both experimentally and numerically. The experimental setup simulates a three-story apartment building (height 6.7 m, width 4 m and depth 1.6 m, with external wall-cladding and a “room fire” at the base. The numerical model was developed in the CFD program Fire Dynamics Simulator (FDS [5] with analogous geometry and instrumentation. The general features of the fire test were well reproduced in the numerical model however temperatures close to the fire source could not be properly accounted for in the model. In this paper the bi-directional probe measurements are elaborated on and the test used in Ref. [1] is revisited using different heat release rates in the numerical model. The velocity of the hot gases along the façade was well reproduced by the simulations although some deviations were found.

  9. Transparent Façade Panel Typologies Based on Recyclable Polymer Materials

    Directory of Open Access Journals (Sweden)

    Harry Giles

    2012-11-01

    describes relevant applications and related current research activities, being carried out by the authors, under an EPA/NSF funded grant project, titled People, Prosperity and Planet, in relation to prototypical composite panel systems. Our current area of investigation relates totypologies that use thermoplastic polymers (as skin material and biocomposites (as a core material. Our evaluations have demonstrated viable applications and improved performance compared to conventional single and double glazing systems in buildings. The paper also discusses the fundamentals of the research investigations and predicts good energy efficiency, making the product a sustainable alternative when used in building applications. The paper highlights areas of ongoing research and applications for hybrid composite façade systems, which will make the approach a viable option for the building industry, in the future.

  10. FA387型并条机性能分析与应用%Performance Analyses and Application of FA387 Drawing Frame

    Institute of Scientific and Technical Information of China (English)

    姜正俊; 袁显政

    2013-01-01

    探讨FA387型并条机的技术性能和应用效果.阐述了FA387型并条机技术规格、性能特点及操作使用方法,并与FA316B型并条机进行了纺纱对比试验.结果表明:FA387型并条机在纺纱质量和牵伸同步性能方面优于FA316B型并条机.认为:运用伺服电机及数字化闭环控制技术,实现主牵伸无级调节和动态监控的高速并条机,具有工艺调整便捷、机构简化、维护方便、改善纺纱质量、提高生产效率、减少用工的特点,代表了高速并条机技术的新趋势.%Technical performance and application effect of FA387 drawing frame were discussed. Technical specifications , performance characteristics and operating use of FA387 drawing frame were introduced, the spinning test was contrasted with FA316B drawing frame. The result shows that FA387 drawing frame is better than that of FA316B drawing frame in aspects of spinning quality and drafting synchronization performance. It is considered that servo motor and digital closed-loop control technology are applied by high-speed drawing frame, the main drafting stepless adjustment and dynamic monitoring can be realized. Performance of this kinds of drawing frame are better, such as convenient process adjustment, simplified structure, easy maintenance, better yarn quality, higher efficiency and less labor. New trend of high-speed drawing machine technology can be represented.

  11. Development and Characterization of a High Sensitivity Segmented Fast Neutron Spectrometer (FaNS-2)

    CERN Document Server

    Langford, T J; Breuer, H; Heimbach, C R; Ji, G; Nico, J S

    2015-01-01

    We present the development of a segmented fast neutron spectrometer (FaNS-2) based upon plastic scintillator and $^3$He proportional counters. It was designed to measure both the flux and spectrum of fast neutrons in the energy range of few MeV to 1 GeV. FaNS-2 utilizes capture-gated spectroscopy to identify neutron events and reject backgrounds. Neutrons deposit energy in the plastic scintillator before capturing on a $^3$He nucleus in the proportional counters. Segmentation improves neutron energy reconstruction while the large volume of scintillator increases sensitivity to low neutron fluxes. A main goal of its design is to study comparatively low neutron fluxes, such as cosmogenic neutrons at the Earth's surface, in an underground environment, or from low-activity neutron sources. In this paper, we present details of its design and construction as well as its characterization with a calibrated $^{252}$Cf source and monoenergetic neutron fields of 2.5 MeV and 14 MeV. Detected monoenergetic neutron spectra...

  12. Climate Based Façade Design for Business Buildings with Examples from Central London

    Directory of Open Access Journals (Sweden)

    John Napier

    2015-01-01

    Full Text Available There is a disconnection between commercial architecture and environmental thinking, where green features can be included as part of a strategy for gaining approvals and marketing projects, but those features are not reviewed after completion and occupation of the building and knowledge is not shared. High levels of air conditioning are still considered unavoidable. Elaborate double skin façades and complex motorized shading systems are adopted; often masking an underlying lack of basic environmental thinking. This article returns (in principle to the physics of comfort in buildings and the passive strategies which can help achieve this with a low energy and carbon footprint. Passive and active façade design strategies are outlined as the basis of a critical tool and a design methodology for new projects. A new architectural sensibility can arise based on modeling the inputs of sunlight, daylight and air temperature in time and space at the early stages of design. Early but sound strategies can be tested and refined using advanced environmental modeling techniques. Architecture and environmental thinking can proceed hand in hand through the design process.

  13. Efeitos da farinha de folhas de mandioca sobre a atividade das enzimas AST, ALT, FA e lipídios hepáticos de ratos Wistar Effects of cassava leaves flour on the AST, ALT, ALP enzymes activity and hepatic lipids of Wistar rats

    Directory of Open Access Journals (Sweden)

    Daniela Séfora de Melo

    2008-12-01

    Full Text Available Folhas de mandioca possuem substâncias como ligninas e saponinas que podem apresentar efeito hipolipidêmico. Todavia, um estudo recente relatou aumento no peso do fígado de ratos alimentados com dietas contendo farinha de folhas de mandioca (FFM - Manihot esculenta Crantz cv. Cacao, tornando-se necessário um estudo mais aprofundado dos efeitos desta farinha sobre os parâmetros hepáticos. Para este estudo, um ensaio biológico com 32 ratos machos Wistar foi conduzido por um período de 7 semanas, sendo os tratamentos: dieta controle e dietas contendo 5, 10 e 15% de FFM. As dietas contendo FFM não apresentaram efeitos sobre as atividades das enzimas Aspartato Aminotransferase (AST e Fosfatase Alcalina (FA, mas aumentaram significativamente a atividade da enzima alanina aminotransferase (ALT. O estudo histopatológico revelou vacuolização do citoplasma dos hepatócitos para todos os grupos. No entanto, a freqüência de animais com vacuolização acentuada foi superior nos grupos que receberam dietas com FFM, apresentando também maiores teores de lipídios e colesterol total hepáticos e maior relação peso fígado/peso corporal. Estes resultados indicam que os antinutrientes presentes nas folhas de mandioca, como taninos, cianeto e saponinas, podem ser responsáveis pela redução da função hepática nos animais alimentados com FFM.Cassava leaves contain substances such as lignins and saponins that can present the hypolipidemic effect. However, a recent study has reported an increase in liver weight of rats fed diet containing cassava leaves flour (CLF - Manihot esculenta Crantz cv. Cacao. Thus, a further study of the effect of this flour on the hepatic parameters is necessary. For the development of this study, a biological assay with 32 male Wistar rats was conducted for a period of 7 weeks with the following treatments: control diet and diets containing 5, 10, and 15% of CLF. The diets containing CLF showed no effects on the

  14. FaDA: Fast Document Aligner using Word Embedding

    Directory of Open Access Journals (Sweden)

    Lohar Pintu

    2016-10-01

    Full Text Available FaDA is a free/open-source tool for aligning multilingual documents. It employs a novel crosslingual information retrieval (CLIR-based document-alignment algorithm involving the distances between embedded word vectors in combination with the word overlap between the source-language and the target-language documents. In this approach, we initially construct a pseudo-query from a source-language document. We then represent the target-language documents and the pseudo-query as word vectors to find the average similarity measure between them. This word vector-based similarity measure is then combined with the term overlap-based similarity. Our initial experiments show that s standard Statistical Machine Translation (SMT- based approach is outperformed by our CLIR-based approach in finding the correct alignment pairs. In addition to this, subsequent experiments with the word vector-based method show further improvements in the performance of the system.

  15. MANOVA, LDA, and FA criteria in clusters parameter estimation

    Directory of Open Access Journals (Sweden)

    Stan Lipovetsky

    2015-12-01

    Full Text Available Multivariate analysis of variance (MANOVA and linear discriminant analysis (LDA apply such well-known criteria as the Wilks’ lambda, Lawley–Hotelling trace, and Pillai’s trace test for checking quality of the solutions. The current paper suggests using these criteria for building objectives for finding clusters parameters because optimizing such objectives corresponds to the best distinguishing between the clusters. Relation to Joreskog’s classification for factor analysis (FA techniques is also considered. The problem can be reduced to the multinomial parameterization, and solution can be found in a nonlinear optimization procedure which yields the estimates for the cluster centers and sizes. This approach for clustering works with data compressed into covariance matrix so can be especially useful for big data.

  16. α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum.

    Science.gov (United States)

    Hu, Weiqun; Zhang, Xiaoping; Chen, Xiang; Zheng, Jingwu; Yin, Yanni; Ma, Zhonghua

    2015-04-01

    The filamentous ascomycete Fusarium asiaticum contains two homologous genes FaTUA1 and FaTUA2 encoding α-tubulins. In this study, we found that FaTUA2 was dispensable for vegetative growth and sporulation in F. asiaticum. The deletion of FaTUA1 however led to dramatically reduced mycelial growth, twisted hyphae and abnormal nuclei in apical cells of hyphae. The FaTUA1 deletion mutant (ΔFaTuA1-5) also showed a significant decrease in conidiation, and produced abnormal conidia. Pathogenicity assays showed that ΔFaTuA1-5 exhibited decreased virulence on wheat head. Unexpectedly, the deletion of FaTUA1 led to resistance to high temperatures. In addition, ΔFaTuA2 showed increased sensitivity to carbendazim. Furthermore, increased FaTUA2 expression in ΔFaTuA1-5 partially restored the defects of the mutant in mycelial growth, conidial production and virulence, vice versa, increased FaTUA1 expression in the FaTUA2 deletion mutant also partially relieved the defect of the mutant in the delay of conidial germination. Taken together, these results indicate that FaTuA1 plays crucial roles in vegetative growth and development, and the functions of FaTuA1 and FaTuA2 are partially interchangeable in F. asiaticum.

  17. Comparison of the inhibition of urokinase-type plasminogen activator (u-PA) activity by monoclonal antibodies specific for u-PA as assessed by different assays

    NARCIS (Netherlands)

    Boheemen, P.A. van; Hoogen, N.M. van den; Koolwijk, N.

    1995-01-01

    Six murine monoclonal antibodies (MAbs) specific for urokinase-type plasminogen activator (u-PA) were tested for their ability to inhibit u-PA activity in three different assays with respect to amidolytic activity, plasminogen activation and fibrinolytic activity. Two of the MAbs were able to inhibi

  18. Liiga väike, et lubada endale fašismi / Tõnis Saarts

    Index Scriptorium Estoniae

    Saarts, Tõnis

    2008-01-01

    Ilmunud ka: Molodjozh Estonii, Vesti Dnja 11. dets. lk. 11,7. Autor nimetab fašismi põhitunnused ning järeldab, et Eesti ei anna ühelgi alusel fašistliku riigi mõõtu välja; fašistlik või poolautoritaarne Eesti saab olla ainult Vene mõju all olev Eesti, ütleb ta

  19. Association of protein kinase FA/GSK-3alpha (a proline-directed kinase and a regulator of protooncogenes) with human cervical carcinoma dedifferentiation/progression.

    Science.gov (United States)

    Yang, S D; Yu, J S; Lee, T T; Ni, M H; Yang, C C; Ho, Y S; Tsen, T Z

    1995-10-01

    Computer analysis of protein phosphorylation-sites sequence revealed that most transcriptional factors and viral oncoproteins are prime targets for regulation of proline-directed protein phosphorylation, suggesting an association of proline-directed protein kinase (PDPK) family with neoplastic transformation and tumorigenesis. In this report, an immunoprecipitate activity assay of protein kinase FA/glycogen synthase kinase-3alpha (kinase FA/GSK-3alpha) (a particular member of PDPK family) has been optimized for human cervical tissue and used to demonstrate for the first time significantly increased (P < 0.001) activity in poorly differentiated cervical carcinoma (82.8 +/- 6.6 U/mg of protein), moderately differentiated carcinoma (36.2 +/- 3.4 U/mg of protein), and well-differentiated carcinoma (18.3 +/- 2.4 U/mg of protein) from 36 human cervical carcinoma samples when compared to 12 normal controls (4.9 +/- 0.6 U/mg of protein). Immunoblotting analysis further revealed that increased activity of kinase FA/GSK-3alpha in cervical carcinoma is due to overexpression of protein synthesis of the kinase. Taken together, the results provide initial evidence that overexpression of protein synthesis and cellular activity of kinase FA/GSK-3alpha may be involved in human cervical carcinoma dedifferentiation/progression, supporting an association of proline-directed protein kinase with neoplastic transformation and tumorigenesis. Since protein kinase FA/GSK-3alpha may function as a possible regulator of transcription factors/proto-oncogenes, the results further suggest that kinase FA/GSK-3alpha may play a potential role in human cervical carcinogenesis, especially in its dedifferentiation and progression.

  20. Effect of façade systems on the performance of cooling ceilings: In situ measurements

    Directory of Open Access Journals (Sweden)

    Katharina Eder

    2015-03-01

    Full Text Available This article presents an innovative façade system designed to increase the thermal comfort inside an office room and to enhance the cooling capacity of the suspended cooling ceiling. A series of measurements is conducted in an existing office building with different façade systems (i.e., a combination of glazing and shading. An innovative façade system is developed based on this intensive set of measurements. The new system enhances the thermal comfort and cooling capacity of the suspended cooling ceiling. The main usage of the new system is the refurbishment and improvement of existing façade systems.

  1. Colour schemes and façade work at Drottningholm and Stockholm palaces Les couleurs des façades des palais royaux de Drottningholm et de Stockholm

    OpenAIRE

    2008-01-01

    Jan Lisinski présente les couleurs des façades des palais royaux de Drottningholm et de Stockholm. Les deux palais furent construits au xviie siècle mais firent l’objet de modifications par la suite. La présentation commence par un bref rappel historique centré sur les différentes couleurs utilisées pour les façades puis aborde la façon dont doivent être traitées les façades aujourd’hui. Il sera question en particulier du traitement des pierres, des plâtres, des maçonneries, des aciers et des...

  2. Colour schemes and façade work at Drottningholm and Stockholm palaces Les couleurs des façades des palais royaux de Drottningholm et de Stockholm

    Directory of Open Access Journals (Sweden)

    Jan Lisinski

    2008-06-01

    Full Text Available Jan Lisinski présente les couleurs des façades des palais royaux de Drottningholm et de Stockholm. Les deux palais furent construits au xviie siècle mais firent l’objet de modifications par la suite. La présentation commence par un bref rappel historique centré sur les différentes couleurs utilisées pour les façades puis aborde la façon dont doivent être traitées les façades aujourd’hui. Il sera question en particulier du traitement des pierres, des plâtres, des maçonneries, des aciers et des cuivres... Les façades des deux palais sont actuellement en restauration mais les solutions mises en œuvre sont différentes. Au palais Royal de Stockholm, le plâtre est simplement nettoyé alors qu’au palais Drottningholm on refait les plâtres puis les peintures à la chaux.Jan Lisinski presents the colours of the façades of the royal palaces of Drottningholm and of Stockholm. The two palaces were constructed in the seventeenth century, but have undergone successive modifications. The presentation begins with a brief historical overview of the different colours used for the façades, then discusses the way the treatments are to be carried out. In particular, the treatment of stone, plaster, masonry, steel and copper will be examined. The façades of the two palaces are currently being restored, but the solutions for each are quite different. For the Royal Palace of Stockholm, the plaster is simply being cleaned, while at Drottningholm Palace, the plaster will be redone, then painted with lime paint.

  3. Insulin like growth factor-1/insulin bypasses Pref-1/FA1-mediated inhibition of adipocyte differentiation

    DEFF Research Database (Denmark)

    Zhang, Hongbin; Nøhr, Jane; Jensen, Charlotte Harken;

    2003-01-01

    of Pref-1/FA1 in 3T3-L1 or 3T3-F442A cells inhibited adipocyte differentiation when insulin or insulin-like growth factor-1 (IGF-1) was omitted from the differentiation mixture. We demonstrate that the level of the mature form of the IGF-1 receptor is reduced and that IGF-1-dependent activation of p42/p44......, and adipocyte differentiation in a dose-dependent manner. Udgivelsesdato: 2003-Jun-6......Pref-1 is a highly glycosylated Delta-like transmembrane protein containing six epidermal growth factor-like repeats in the extracellular domain. Pref-1 is abundantly expressed in preadipocytes, but expression is down-regulated during adipocyte differentiation. Forced expression of Pref-1 in 3T3-L1...

  4. Cellular characterization of cells from the Fanconi anemia complementation group, FA-D1/BRCA2

    Energy Technology Data Exchange (ETDEWEB)

    Godthelp, Barbara C. [Department of Toxicogenetics, Leiden University Medical Center, Building 2, Postzone S-6-P, P.O. Box 9600, 2300 RC, Leiden (Netherlands); Buul, Paul P.W. van [Department of Toxicogenetics, Leiden University Medical Center, Building 2, Postzone S-6-P, P.O. Box 9600, 2300 RC, Leiden (Netherlands); Jaspers, Nicolaas G.J. [Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam (Netherlands); Elghalbzouri-Maghrani, Elhaam [Department of Toxicogenetics, Leiden University Medical Center, Building 2, Postzone S-6-P, P.O. Box 9600, 2300 RC, Leiden (Netherlands); Duijn-Goedhart, Annemarie van [Department of Toxicogenetics, Leiden University Medical Center, Building 2, Postzone S-6-P, P.O. Box 9600, 2300 RC, Leiden (Netherlands); Arwert, Fre [Department of Clinical Genetics and Human Genetics, Free University Medical Center, Amsterdam (Netherlands); Joenje, Hans [Department of Clinical Genetics and Human Genetics, Free University Medical Center, Amsterdam (Netherlands); Zdzienicka, Malgorzata Z. [Department of Toxicogenetics, Leiden University Medical Center, Building 2, Postzone S-6-P, P.O. Box 9600, 2300 RC, Leiden (Netherlands) and Department of Molecular Cell Genetics, Collegium Medicum, N.Copernicus University, Bydgoszcz (Poland)]. E-mail: M.Z.Zdzienicka@LUMC.nl

    2006-10-10

    Fanconi anemia (FA) is an inherited cancer-susceptibility disorder, characterized by genomic instability and hypersensitivity to DNA cross-linking agents. The discovery of biallelic BRCA2 mutations in the FA-D1 complementation group allows for the first time to study the characteristics of primary BRCA2-deficient human cells. FANCD1/BRCA2-deficient fibroblasts appeared hypersensitive to mitomycin C (MMC), slightly sensitive to methyl methane sulfonate (MMS), and like cells derived from other FA complementation groups, not sensitive to X-ray irradiation. However, unlike other FA cells, FA-D1 cells were slightly sensitive to UV irradiation. Despite the observed lack of X-ray sensitivity in cell survival, significant radioresistant DNA synthesis (RDS) was observed in the BRCA2-deficient fibroblasts but also in the FANCA-deficient fibroblasts, suggesting an impaired S-phase checkpoint. FA-D1/BRCA2 cells displayed greatly enhanced levels of spontaneous as well as MMC-induced chromosomal aberrations (Canada), similar to cells deficient in homologous recombination (HR) and non-D1 FA cells. In contrast to Brca2-deficient rodent cells, FA-D1/BRCA2 cells showed normal sister chromatid exchange (SCE) levels, both spontaneous as well as after MMC treatment. Hence, these data indicate that human cells with biallelic BRCA2 mutations display typical features of both FA- and HR-deficient cells, which suggests that FANCD1/BRCA2 is part of the integrated FA/BRCA DNA damage response pathway but also controls other functions outside the FA pathway.

  5. Construction and Characterization of Novel Staphylokinase Variants with Antiplatelet Aggregation Activity and Reduced Immunogenecity

    Institute of Scientific and Technical Information of China (English)

    Hua-Bo SU; Yu-Gao ZHANG; Jin-Tian HE; Wei MO; Yan-Ling ZHANG; Xian-Mei TAO; Hou-Yan SONG

    2004-01-01

    To develop target thrombolytic agents with fibrinolytic activity, antiplatelet aggregation activity and reduced immunogenicity, two staphylokinase variants containing Arg-Gly-Asp (RGD) motif were constructed. Gene expression was induced in E. coli JF1125 and the variants, designated DGR and RL1, were purified with gel filtration and ion-exchange chromatography and the purity was over 95%. The fibrinolytic activity and kinetic constants of the two variants were comparable to those of recombinant wild-type staphylokinase. Both the variants can inhibit the platelet aggregation at a final concentration of 2 μM. Thetiters of antibodies against variants were much lower than those against recombinant staphylokinase in guineapigs, which indicated that the immunogenicity of the variants was greatly reduced. These results confirm thatit is possible to design and produce a bifunctional protein that possesses fibrinolytic and antiplatelet aggregation activities.

  6. Diffusion tensor MRI tractography reveals increased fractional anisotropy (FA) in arcuate fasciculus following music-cued motor training.

    Science.gov (United States)

    Moore, Emma; Schaefer, Rebecca S; Bastin, Mark E; Roberts, Neil; Overy, Katie

    2017-08-01

    Auditory cues are frequently used to support movement learning and rehabilitation, but the neural basis of this behavioural effect is not yet clear. We investigated the microstructural neuroplasticity effects of adding musical cues to a motor learning task. We hypothesised that music-cued, left-handed motor training would increase fractional anisotropy (FA) in the contralateral arcuate fasciculus, a fibre tract connecting auditory, pre-motor and motor regions. Thirty right-handed participants were assigned to a motor learning condition either with (Music Group) or without (Control Group) musical cues. Participants completed 20minutes of training three times per week over four weeks. Diffusion tensor MRI and probabilistic neighbourhood tractography identified FA, axial (AD) and radial (RD) diffusivity before and after training. Results revealed that FA increased significantly in the right arcuate fasciculus of the Music group only, as hypothesised, with trends for AD to increase and RD to decrease, a pattern of results consistent with activity-dependent increases in myelination. No significant changes were found in the left ipsilateral arcuate fasciculus of either group. This is the first evidence that adding musical cues to movement learning can induce rapid microstructural change in white matter pathways in adults, with potential implications for therapeutic clinical practice. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  7. Solar Flare Measurements with STIX and MiSolFA

    CERN Document Server

    Casadei, Diego

    2014-01-01

    Solar flares are the most powerful events in the solar system and the brightest sources of X-rays, often associated with emission of particles reaching the Earth and causing geomagnetic storms, giving problems to communication, airplanes and even black-outs. X-rays emitted by accelerated electrons are the most direct probe of solar flare phenomena. The Micro Solar-Flare Apparatus (MiSolFA) is a proposed compact X-ray detector which will address the two biggest issues in solar flare modeling. Dynamic range limitations prevent simultaneous spectroscopy with a single instrument of all X-ray emitting regions of a flare. In addition, most X-ray observations so far are inconsistent with the high anisotropy predicted by the models usually adopted for solar flares. Operated at the same time as the STIX instrument of the ESA Solar Orbiter mission, at the next solar maximum (2020), they will have the unique opportunity to look at the same flare from two different directions: Solar Orbiter gets very close to the Sun wit...

  8. Façade fires in Swedish school buildings

    Directory of Open Access Journals (Sweden)

    Johansson Nils

    2013-11-01

    Full Text Available Fires outside school buildings caused by arson are a major problem in Sweden. The initial fire source can involve rubbish or other types of combustibles that can be found at or sometimes brought to the school. As the external fire develops the flames will extend along the façade. If the building is a one-story building, which is a common construction in Swedish school buildings, the fire can spread to the attic and further on to the rest of the building. A large research project was initiated in 2008 by the Swedish Fire Research Board in order to reduce the number and consequences of arson fires in school buildings. In this paper an evaluation of some technical systems that can be used to mitigate consequences of exterior fires is presented. The evaluation includes a literature review, experimental studies and a cost-benefit analysis. The study showed that some systems have clear cost-benefit advantages for the protection of school buildings in places where a high fire frequency can be expected.

  9. The dam replacing gene product enhances Neisseria gonorrhoeae FA1090 viability and biofilm formation.

    Science.gov (United States)

    Kwiatek, Agnieszka; Bacal, Pawel; Wasiluk, Adrian; Trybunko, Anastasiya; Adamczyk-Poplawska, Monika

    2014-01-01

    Many Neisseriaceae do not exhibit Dam methyltransferase activity and, instead of the dam gene, possess drg (dam replacing gene) inserted in the leuS/dam locus. The drg locus in Neisseria gonorrhoeae FA1090 has a lower GC-pairs content (40.5%) compared to the whole genome of N. gonorrhoeae FA1090 (52%). The gonococcal drg gene encodes a DNA endonuclease Drg, with GmeATC specificity. Disruption of drg or insertion of the dam gene in gonococcal genome changes the level of expression of genes as shown by transcriptome analysis. For the drg-deficient N. gonorrhoeae mutant, a total of 195 (8.94% of the total gene pool) genes exhibited an altered expression compared to the wt strain by at least 1.5 fold. In dam-expressing N. gonorrhoeae mutant, the expression of 240 genes (11% of total genes) was deregulated. Most of these deregulated genes were involved in translation, DNA repair, membrane biogenesis and energy production as shown by cluster of orthologous group analysis. In vivo, the inactivation of drg gene causes the decrease of the number of live neisserial cells and long lag phase of growth. The insertion of dam gene instead of drg locus restores cell viability. We have also shown that presence of the drg gene product is important for N. gonorrhoeae FA1090 in adhesion, including human epithelial cells, and biofilm formation. Biofilm produced by drg-deficient strain is formed by more dispersed cells, compared to this one formed by parental strain as shown by scanning electron and confocal microscopy. Also adherence assays show a significantly smaller biomass of formed biofilm (OD570 = 0.242 ± 0.038) for drg-deficient strain, compared to wild-type strain (OD570 = 0.378 ± 0.057). Dam-expressing gonococcal cells produce slightly weaker biofilm with cells embedded in an extracellular matrix. This strain has also a five times reduced ability for adhesion to human epithelial cells. In this context, the presence of Drg is more advantageous for N. gonorrhoeae biology than

  10. Identification and Characterization of FaFT1: A Homolog of FLOWERING LOCUS T from Strawberry

    Directory of Open Access Journals (Sweden)

    Hengjiu Lei

    2015-05-01

    Full Text Available FLOWERING LOCUS T(FT -like genes play crucial roles in flowering transition in several plant species. In this study, a homolog of FT, designated as FaFT1, was isolated and characterized from strawberry. The open reading frame of FaFT1 was 531 bp, encoding a protein of 176 amino acids. Phylogenetic and sequence analysis showed that the FaFT1 protein contained the conservation of Tyr84 and Gln139, as well as the highly conserved amino acid sequences LGRQTVYAPGWRQN and LYN and that it was a member of the FT-like genes of dicots. Subcellular localization analysis revealed that the FaFT1 protein mainly localized in the nuclei of the Arabidopsis protoplasts. FaFT1 was highly expressed in strawberry mature leaves and its expression level decreased under floral induction conditions. Additionally, FaFT1 expression exhibited diurnal circadian rhythm both under SD and LD conditions. Over expression of FaFT1 in wild-type Arabidopsis caused early flowering. Taken together, these results indicate that FaFT1 is a putative FT homolog in strawberry, acting as a floral promoter in Arabidopsis.

  11. FANCM-FAAP24 and FANCJ: FA proteins that metabolize DNA

    Energy Technology Data Exchange (ETDEWEB)

    Ali, Abdullah Mahmood; Singh, Thiyam Ramsing [Division of Experimental Hematology and Cancer Biology, Cincinnati Children' s Research Foundation, Cincinnati Children' s Hospital Medical Center, Cincinnati, OH 45229 (United States); Meetei, Amom Ruhikanta, E-mail: Ruhikanta.Meetei@cchmc.org [Division of Experimental Hematology and Cancer Biology, Cincinnati Children' s Research Foundation, Cincinnati Children' s Hospital Medical Center, Cincinnati, OH 45229 (United States); Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229 (United States)

    2009-07-31

    Fanconi anemia (FA) is a rare autosomal recessive or X-linked disorder characterized by aplastic anemia, cancer susceptibility and cellular sensitivity to DNA-crosslinking agents. Eight FA proteins (FANCA, -B, -C, -E, -F, -G, -L and -M) and three non-FA proteins (FAAP100, FAAP24 and HES1) form the FA nuclear core complex that is required for monoubiquitination of the FANCD2-FANCI dimer upon DNA damage. The other three FA proteins, FANCD1/BRCA2, FANCJ/BACH1/BRIP1 and FANCN/PALB2, act in parallel or downstream of the FANCD2-FANCI dimer. Despite the isolation and characterization of several FA proteins, the mechanism by which these proteins protect cells from DNA interstrand crosslinking agents has been unclear. This is because a majority of the FA proteins lack any recognizable functional domains that can provide insight into their function. The recently discovered FANCM (Hef) and FANCJ (BRIP1/BACH1) proteins contain helicase domains, providing potential insight into the role of FA proteins in DNA repair. FANCM with its partner, FAAP24, and FANCJ bind and metabolize a variety of DNA substrates. In this review, we focus on the discovery, structure, and function of the FANCM-FAAP24 and FANCJ proteins.

  12. Does Fetal antigen 1 (FA1) identify cells with regenerative, endocrine and neuroendocrine potentials?

    DEFF Research Database (Denmark)

    Jensen, Charlotte Floridon; Jensen, Charlotte Harken; Thorsen, Poul

    2000-01-01

    in the subcellular localisation indicating differential post-translational/post-transcriptional modifications during fetal development. FA1 may be a new marker of cellular subtypes with a regenerative potential and of specific cells with endocrine or neuroendocrine functions. Udgivelsesdato: 2000-Aug......, the localisation of FA1/dlk was analysed in embryonic and fetal tissues between week 5 to 25 of gestation and related to germinal origin and development. FA1 was observed in endodermally derived hepatocytes, glandular cells of the pancreas anlage, and in respiratory epithelial cells. FA1 was also present...... in mesodermally derived cells of the renal proximal tubules, adrenal cortex, Leydig and Hilus cells of the testes and ovaries, fetal chondroblasts, and skeletal myotubes. Ectodermally derived neuro- and adenohypophysial cells, cells in the floor of the 3rd ventricle and plexus choroideus were also FA1 positive...

  13. Thyromimetic actions of tetrabromobisphenol A (TBBPA) in steatotic FaO rat hepatoma cells.

    Science.gov (United States)

    Grasselli, E; Cortese, K; Fabbri, R; Smerilli, A; Vergani, L; Voci, A; Gallo, G; Canesi, L

    2014-10-01

    Tetrabromobisphenol A (2,2-bis(3,5-dibromo-4-hydroxyphenyl propane-TBBPA) is the most produced brominated flame retardant, detected in the environment and in biological samples. TBBPA shares structural similarities with thyroid hormones (THs), and it has been shown to interfere with different aspects of TH physiology, this raising concern on its possible effects as an endocrine disruptor in humans and wildlife. THs play a major role in lipid metabolism, with the liver representing one of their main target tissues. At the cellular level, THs act through interactions with TH receptors (TRs), as well as through TR-independent mechanisms. Rat hepatoma FaO cells (a liver cell line defective for functional TRs) overloaded with lipids have been utilized as a model to investigate the anti-steatotic effects of THs in the hepatocyte. In this work, the possible effects of TBBPA in steatotic FaO cells were investigated. Exposure to TBBPA for 24 h reduced triglyceride (TAG) content and the size of lipid droplets (LDs); similar effects were obtained with equimolar doses (10(-6) M) of T3 (3,3',5-L-triiodothyronine). TBBPA and T3 showed common effects on transcription of genes involved in lipid homeostasis. In particular, TBBPA mainly up-regulated mRNA levels for LD-associated oxidative tissue-enriched PAT protein (OXPAT), peroxisome proliferator-activated receptor (PPAR) isoform β/δ, and the mitochondrial uncoupling protein 2 (UCP2). The results demonstrate that TBBPA can decrease lipid accumulation in steatotic cells through stimulation of oxidative pathways. These data identify novel thyromimetic actions of TBBPA at the cellular level.

  14. The effect of single agent oral fusaric acid (FA) on the growth of subcutaneously xenografted SCC-1 cells in a nude mouse model.

    Science.gov (United States)

    Ruda, James M; Beus, Kirt S; Hollenbeak, Christopher S; Wilson, Ronald P; Stack, Brendan C

    2006-09-01

    To determine whether oral administration of fusaric acid (FA) inhibits tumor growth in an animal model of head and neck cancer (HNSCC). In vivo murine model, two arm controlled study. Thirty-eight (38) 5-week-old athymic nude mice were randomly assigned to a fusaric acid treatment group (1 mg/mL) (n = 19) or a sterile saline group (n = 19). A left, lateral flank subcutaneous injection of 2.0 x 10(6) UM-SCC-1 cells were administered to all mice on day 1. Both groups were gavaged daily with either 0.25 mLs of oral FA or sterile saline throughout the experiment (32 days). Latency to a measurable tumor (> or =65 mm3), and tumor volumes were recorded after tumor xenografting. Tumor weights were recorded at the conclusion of the experiment. Tumor volume growth curves were modeled as polynomial functions of time with treatment interaction effects. Survivorship functions for time to measurable tumor were estimated using the Kaplan-Meier product limit estimator. Survival analysis showed mice treated with FA developed measurable tumors after a significantly longer interval post-xenografting than control mice (p = 0.00451). By Day 9, all mice in the control group had developed measurable tumors in comparison to only 78% of mice in the FA group. Likewise, estimated growth curves for both groups suggested that mice receiving FA demonstrated significantly slower tumor growth rates throughout the entire study period (p < 0.0001). At the conclusion of the experiment, tumor weights from both the control and FA groups were also significantly different (p = 0.0142). Single agent oral fusaric acid (1 mg/mL) is an inhibitor of UM-SCC-1 in a murine model. As an orally active agent, it may have a potential role in the treatment of human squamous cell carcinoma of the head and neck.

  15. A Non-Ventilated Solar Façade Concept Based on Selective and Transparent Insulation Material Integration: An Experimental Study

    Directory of Open Access Journals (Sweden)

    Miroslav Čekon

    2017-06-01

    Full Text Available A new solar façade concept based on transparent insulation and a selective absorber is proposed, tested and compared with conventional insulation and a non-selective type of absorber, respectively. The presented study focuses on an experimental non-ventilated solar type of façade exposed to solar radiation both in the laboratory and in outdoor tests. Due to the high solar absorbance level of the façade, high- and low-emissivity contributions were primarily analysed. All of the implemented materials were contrasted from the thermal and optical point of view. An analysis was made of both thermodynamic and steady state procedures affecting the proposed solar façade concept. Experimental full scale tests on real building components were additionally involved during summer monitoring. An indicator of the temperature response generated by solar radiation exposure demonstrates the outdoor performance of the façade is closely related to overheating phenomena. From the thermal point of view, the proposed transparent insulation and selective absorber concept corresponds to the performance of conventional thermal insulation of identical material thickness; however, the non-selective prototype only provides 50% thermal performance. The results of the solar-based experiments show that with a small-scale experimental prototype, approximately no significant difference is measured when compared with a non-selective absorber type. The only difference was achieved at the maximum of 2.5 K, when the lower temperature was obtained in the solar selective concept. At the full-scale outdoor mode, the results indicate a maximum of 3.0 K difference, however the lower temperature achieves a non-selective approach. This solar façade can actively contribute to the thermal performance of building components during periods of heating.

  16. FaQR, required for the biosynthesis of the strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone, encodes an enone oxidoreductase.

    Science.gov (United States)

    Raab, Thomas; López-Ráez, Juan Antonio; Klein, Dorothée; Caballero, Jose Luis; Moyano, Enriqueta; Schwab, Wilfried; Muñoz-Blanco, Juan

    2006-04-01

    The flavor of strawberry (Fragaria x ananassa) fruit is dominated by an uncommon group of aroma compounds with a 2,5-dimethyl-3(H)-furanone structure. We report the characterization of an enzyme involved in the biosynthesis of 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF; Furaneol), the key flavor compound in strawberries. Protein extracts were partially purified, and the observed distribution of enzymatic activity correlated with the presence of a single polypeptide of approximately 37 kD. Sequence analysis of two peptide fragments showed total identity with the protein sequence of a strongly ripening-induced, auxin-dependent putative quinone oxidoreductase, Fragaria x ananassa quinone oxidoreductase (FaQR). The open reading frame of the FaQR cDNA consists of 969 bp encoding a 322-amino acid protein with a calculated molecular mass of 34.3 kD. Laser capture microdissection followed by RNA extraction and amplification demonstrated the presence of FaQR mRNA in parenchyma tissue of the strawberry fruit. The FaQR protein was functionally expressed in Escherichia coli, and the monomer catalyzed the formation of HDMF. After chemical synthesis and liquid chromatography-tandem mass spectrometry analysis, 4-hydroxy-5-methyl-2-methylene-3(2H)-furanone was confirmed as a substrate of FaQR and the natural precursor of HDMF. This study demonstrates the function of the FaQR enzyme in the biosynthesis of HDMF as enone oxidoreductase and provides a foundation for the improvement of strawberry flavor and the biotechnological production of HDMF.

  17. RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP).

    Science.gov (United States)

    Zhao, Chi; Gan, Fei; Shen, Gaozhong; Bryant, Donald A

    2015-01-01

    Terrestrial cyanobacteria often occur in niches that are strongly enriched in far-red light (FRL; λ > 700 nm). Some cyanobacteria exhibit a complex and extensive photoacclimation response, known as FRL photoacclimation (FaRLiP). During the FaRLiP response, specialized paralogous proteins replace 17 core subunits of the three major photosynthetic complexes: Photosystem (PS) I, PS II, and the phycobilisome. Additionally, the cells synthesize both chlorophyll (Chl) f and Chl d. Using biparental mating from Escherichia coli, we constructed null mutants of three genes, rfpA, rfpB, and rfpC, in the cyanobacteria Chlorogloeopsis fritschii PCC 9212 and Chroococcidiopsis thermalis PCC 7203. The resulting mutants were no longer able to modify their photosynthetic apparatus to absorb FRL, were no longer able to synthesize Chl f, inappropriately synthesized Chl d in white light, and were unable to transcribe genes of the FaRLiP gene cluster. We conclude that RfpA, RfpB, and RfpC constitute a FRL-activated signal transduction cascade that is the master control switch for the FaRLiP response. FRL is proposed to activate (or inactivate) the histidine kinase activity of RfpA, which leads to formation of the active state of RfpB, the key response regulator and transcription activator. RfpC may act as a phosphate shuttle between RfpA and RfpB. Our results show that reverse genetics via conjugation will be a powerful approach in detailed studies of the FaRLiP response.

  18. Volatile organic compounds produced by a soil-isolate, Bacillus subtilis FA26 induce adverse ultra-structural changes to the cells of Clavibacter michiganensis ssp. sepedonicus, the causal agent of bacterial ring rot of potato.

    Science.gov (United States)

    Rajer, Faheem Uddin; Wu, Huijun; Xie, Yongli; Xie, Shanshan; Raza, Waseem; Tahir, Hafiz Abdul Samad; Gao, Xuewen

    2017-04-01

    Rhizobacterial volatile organic compounds (VOCs) play an important role in the suppression of soil-borne phytopathogens. In this study, the VOCs produced by a soil-isolate, Bacillus subtilis FA26, were evaluated in vitro for their antibacterial activity against Clavibacter michiganensis ssp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato. The VOCs emitted by FA26 inhibited the growth of Cms significantly compared with the control. Scanning and transmission electron microscopy analyses revealed distorted colony morphology and a wide range of abnormalities in Cms cells exposed to the VOCs of FA26. Varying the inoculation strategy and inoculum size showed that the production and activity of the antibacterial VOCs of FA26 were dependent on the culture conditions. Headspace solid-phase microextraction/gas chromatography-mass spectrometry analyses revealed that FA26 produced 11 VOCs. Four VOCs (benzaldehyde, nonanal, benzothiazole and acetophenone) were associated with the antibacterial activity against Cms. The results suggested that the VOCs produced by FA26 could control the causal agent of bacterial ring rot of potato. This information will increase our understanding of the microbial interactions mediated by VOCs in nature and aid the development of safer strategies for controlling plant disease.

  19. Oceanographic profile data collected from CTD casts aboard FA2806 and NOAA Ship FAIRWEATHER as part of project OPR-Q328-FA-15 in the Bering Sea on 2015-07-08 (NCEI Accession 0130934)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0130934 includes physical and profile data collected aboard the FA2806 and NOAA Ship FAIRWEATHER during project OPR-Q328-FA-15 in the Bering Sea on...

  20. Metabolic and fibrinolytic response to changed insulin sensitivity in users of oral contraceptives

    DEFF Research Database (Denmark)

    Petersen, Kresten R.; Christiansen, Erik; Madsbad, Sten

    1999-01-01

    randomization to 6 months of treatment with ethinyl estradiol in triphasic combination with norgestimate (n = 17) or gestodene (n = 20). Measurements of fasting triglycerides and antigen concentrations of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor type 1 (PAI-1) were also...

  1. Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2.

    Science.gov (United States)

    Yamada, F; Takahashi, N; Murachi, T

    1976-06-01

    Fruit bromelain FA2, the main proteinase component of the juice of pineapple fruit, has been purified and characterized. 1. Efficient extraction of this enzyme from the crude material was possible using "Cellulosin AP," a microbial polysaccharidase preparation containing cellulase, hemicellulase, and pectinase. The enzyme was purified mainly by successive applications of anion-exchange chromatography, yielding an apparently homogeneous protein as judged by several physical, chemical, and immunochemical criteria. Properties of FA2 include: molecular weight, 31,000; isoelectric point, pH 4.6; absorbance at 280 nm of a 1% solution at pH 7.0 per cm, 19.2. 2. FA2 gave only alanine phenylthiohydantoin upon amino-terminal group analysis by the Edman procedure. Stepwise degradation yielded the amino-terminal sequence Ala-Val-Pro-Gln-Ser-Ile-Asp-Trp-Arg-Asp-Tyr-Gly-Ala. The amino acid composition of FA2 was not markedly different from that of stem bromelain, except for a much smaller lysine content and a smaller alanine content relative to glycine in FA2. FA2 contained neither amino sugars nor neutral carbohydrates as determined by several methods, so FA2 is not a glycoprotein. 3. By labeling the reactive cysteine residue (CYS) with [14C]iodoacetate, the following partial amino acid sequence has been determined. Asn-Glx-Asn-Pro-Cys-Gly-Ala-CYS.

  2. INTERACTION OF STREPTOCOCCAL PLASMINOGEN BINDING PROTEINS WITH THE HOST FIBRINOLYTIC SYSTEM

    Directory of Open Access Journals (Sweden)

    Marcus eFulde

    2013-11-01

    Full Text Available The ability to take advantage of plasminogen and its activated form plasmin is a common mechanism used by commensal as well as pathogenic bacteria in interaction with their respective host. Hence, a huge variety of plasminogen binding proteins and/or activation mechanisms exist. This review solely focuses on the genus Streptococcus and, in particular, on the so-called non-activating plasminogen binding proteins. Based on structural and functional differences, as well as on their mode of surface linkaging, three groups can be assigned: M-(like proteins, surface displayed cytoplasmatic proteins with enzymatic activities ("moonlighting proteins" and other surface proteins. Here, the plasminogen binding sites and the interaction mechanisms are compared. Recent findings on the functional consequences of these interactions on tissue degradation and immune evasion are summarized.

  3. Effects of inhaled plasminogen activator on the balance between coagulation and fibrinolysis in traumatized pigs

    DEFF Research Database (Denmark)

    Münster, A-M B; Rasmussen, L; Sidelmann, Johannes Jakobsen

    2002-01-01

    A profibrinolytic state is normal in the alveoli, but this may change as a result of trauma, possibly leading to fibrin deposition, a characteristic of acute lung injury/acute respiratory distress syndrome. Therefore, the present study investigated in a double-blind, placebo-controlled manner...... with an increased plasmin-dependent fibrinolytic activity without increased systemic fibrinolytic activity, the transient increase in the concentration of scu-PA in the plasma being minimal. In conclusion, the study shows that activatable scu-PA can be nebulized to the lower respiratory tract and can increase...

  4. Desalination of Walls and Façades

    Science.gov (United States)

    Wedekind, W.; Jáuregui Arreola, K.; Siegesmund, S.

    2012-04-01

    For large monumental objects like walls and façades, the common technique of applying poultices for desalination often are not effective. This practice is neither cost effective nor does it lead to the desired result of desalination. To manage the conservation and desalination of these kinds of objects, several sprinkling techniques are known and have been applied on historical objects. For example, in the wooden warship Vasa, which was excavated from the sea bottom in Stockholm/Sweden, a sprinkling method was applied in 1961 for conservation and desalination. A sprinkling method to desalinate porous mineral materials will be presented using three different case studies: the rock cut monument no. 825 in Petra/Jordan, the medieval monastary church of the former Franziscan convent in Zeitz/Germany and the baroque monastary church Santa Monica in Guadalajara/Mexico. Before to start with practical conservation, the material- and petropysical properties, focoussed on water transport properties, like porosity, pore size distribution, water uptake and drying rate were investigadet. Diagnostic investigations on the objects included the mapping of deterioration, moister content measurements and salt accumulation determined by borehole cuts samples at depth. In the sprinkling method water is sprayed onto the wall surface through nozzels arranged in a modular grid. Depending on the sprinkling duration, a small or a large amount of water seeps into the porous materials, whereby the depth penetration can be adjusted accordingly. The water not absorbed by the stone runs off the facade and can be collected in liter amounts and tested by electrical conductivity with respect to the dissolved substances. After the drying of the wall's surface and the accumulation of salt at the material's surface, the procedure is repeated. For each subsequent washing a lower content of salt should be brought to the surface. Step by step the salt concentration will eventually decrease to almost

  5. Disrupting Façades of Clarity in the Teaching and Learning of Qualitative Research

    Directory of Open Access Journals (Sweden)

    Melissa Contreras-McGavin

    2013-02-01

    Full Text Available In this article we examine two methodological façades of clarity that commonly shroud critical qualitative educational inquiry. More specifically, we interrogate discussions of reflexivity and positionality and explore the ways in which methodology curricula and instructional practices perpetuate façades of clarity, or a false sense of coherence, ultimately undermining the transformative potential of critical educational research. We identify specific pedagogical opportunities, spaces, and strategies for dismantling these façades and offer ways to reconstruct methodological practices congruent with the emancipatory and empowering aims of critical scholarship.

  6. FaRe: A Mathematica package for tensor reduction of Feynman integrals

    Science.gov (United States)

    Re Fiorentin, Michele

    2016-08-01

    In this paper, we present FaRe, a package for Mathematica that implements the decomposition of a generic tensor Feynman integral, with arbitrary loop number, into scalar integrals in higher dimension. In order for FaRe to work, the package FeynCalc is needed, so that the tensor structure of the different contributions is preserved and the obtained scalar integrals are grouped accordingly. FaRe can prove particularly useful when it is preferable to handle Feynman integrals with free Lorentz indices and tensor reduction of high-order integrals is needed. This can then be achieved with several powerful existing tools.

  7. FaRe: a Mathematica package for tensor reduction of Feynman integrals

    CERN Document Server

    Fiorentin, Michele Re

    2015-01-01

    We present FaRe, a package for Mathematica that implements the decomposition of a generic tensor Feynman integral, with arbitrary loop number, into scalar integrals in higher dimension. In order for FaRe to work, the package FeynCalc is needed, so that the tensor structure of the different contributions is preserved and the obtained scalar integrals are grouped accordingly. FaRe can prove particularly useful when it is preferable to handle Feynman integrals with free Lorentz indices and tensor reduction of high-order integrals is needed. This can then be achieved with several powerful existing tools.

  8. Estrogen inhibits Dlk1/FA1 production: A potential mechanism for estrogen effects on bone turnover

    DEFF Research Database (Denmark)

    Abdallah, Basem M; Bay-Jensen, Anne-Christine; Srinivasan, Bhuma;

    2011-01-01

    We have recently identified delta-like 1/fetal antigen 1 (Dlk1/FA1) as a novel regulator of bone mass that functions to mediate bone loss under estrogen deficiency in mice. In this report, we investigated the effects of estrogen (E) deficiency and E replacement on serum (s) levels of Dlk1/FA1 (s...... estrogen-replacement therapy (ERT, n = 166). s-Dlk1/FA1 and s-CTX were elevated in postmenopausal E-deficient women compared with premenopausal E-replete women (both p ...

  9. Trending Fibrinolytic Dysregulation: Fibrinolysis Shutdown in the Days After Injury Is Associated With Poor Outcome in Severely Injured Children.

    Science.gov (United States)

    Leeper, Christine M; Neal, Matthew D; McKenna, Christine J; Gaines, Barbara A

    2017-09-01

    To trend fibrinolysis after injury and determine the influence of traumatic brain injury (TBI) and massive transfusion on fibrinolysis status. Admission fibrinolytic derangement is common in injured children and adults, and is associated with poor outcome. No studies examine fibrinolysis days after injury. Prospective study of severely injured children at a level 1 pediatric trauma center. Rapid thromboelastography was obtained on admission and daily for up to 7 days. Standard definitions of hyperfibrinolysis (HF; LY30 ≥3), fibrinolysis shutdown (SD; LY30 ≤0.8), and normal (LY30 = 0.9-2.9) were applied. Antifibrinolytic use was documented. Outcomes were death, disability, and thromboembolic complications. Wilcoxon rank-sum and Fisher exact tests were performed. Exploratory subgroups included massively transfused and severe TBI patients. In all, 83 patients were analyzed with median (interquartile ranges) age 8 (4-12) and Injury Severity Score 22 (13-34), 73.5% blunt mechanism, 47% severe TBI, 20.5% massively transfused. Outcomes were 14.5% mortality, 43.7% disability, and 9.8% deep vein thrombosis. Remaining in or trending to SD was associated with death (P = 0.007), disability (P = 0.012), and deep vein thrombosis (P = 0.048). Median LY30 was lower on post-trauma day (PTD)1 to PTD4 in patients with poor compared with good outcome; median LY30 was lower on PTD1 to PTD3 in TBI patients compared with non-TBI patients. HF without associated shutdown was not related to poor outcome, but extreme HF (LY30 >30%, n = 3) was lethal. Also, 50% of massively transfused patients in hemorrhagic shock demonstrated SD physiology on admission. All with HF (fc31.2%) corrected after hemostatic resuscitation without tranexamic acid. Fibrinolysis shutdown is common postinjury and predicts poor outcomes. Severe TBI is associated with sustained shutdown. Empiric antifibrinolytics for children should be questioned; thromboelastography-directed selective use should be considered for

  10. Certification of Charpy V-notch Reference Test Pieces of 80 J Nominal Absorbed Energy (ERM®-FA015x and ERM®-FA015y)

    OpenAIRE

    LAMBERTY MARIE ANDREE; Dean, Alan; Roebben, Gert

    2011-01-01

    This certification report describes the processing and characterisation of ERM®-FA015x and ERM®-FA015y, two batches of Charpy V-notch certified reference test pieces. Sets of five of these test pieces are used for the verification of pendulum impact test machines according to EN 10045-2 (Charpy impact test on metallic materials, Part 2. Method for the verification of impact testing machines) or according to ISO 148-2 (Metallic materials - Charpy pendulum impact test – Part 2: Verification of ...

  11. Fibrinolytic Therapy Versus Primary Percutaneous Coronary Interventions for ST-Segment Elevation Myocardial Infarction in Kentucky: Time to Establish Systems of Care?

    Science.gov (United States)

    Wallace, Eric L.; Kotter, John R.; Charnigo, Richard; Kuvlieva, Liliana B.; Smyth, Susan S.; Ziada, Khaled M.; Campbell, Charles L.

    2013-01-01

    Background Fibrinolytic therapy is recommended for ST-segment myocardial infarctions (STEMI) when primary percutaneous coronary intervention (PPCI) is not available or cannot be performed in a timely manner. Despite this recommendation, patients often are transferred to PPCI centers with prolonged transfer times, leading to delayed reperfusion. Regional approaches have been developed with success and we sought to increase guideline compliance in Kentucky. Methods A total of 191 consecutive STEMI patients presented to the University of Kentucky (UK) Chandler Medical Center between July 1, 2009 and June 30, 2011. The primary outcome was in-hospital mortality and the secondary outcomes were major adverse cardiovascular events, extent of myocardial injury, bleeding, and 4) length of stay. Patients were analyzed by presenting facility—the UK hospital versus an outside hospital (OSH)—and treatment strategy (PPCI vs fibrinolytic therapy). Further analyses assessed primary and secondary outcomes by treatment strategy within transfer distance and compliance with American Heart Association guidelines. Results Patients presenting directly to the UK hospital had significantly shorter door-to-balloon times than those presenting to an OSH (83 vs 170 minutes; P P = 0.45). Overall, only 20% of OSH patients received timely reperfusion, 13% PPCI, and 42% fibrinolytics. In a multivariable model, delayed reperfusion significantly predicted major adverse cardiovascular events (odds ratio 3.87, 95% confidence interval 1.15–13.0; P = 0.02), whereas the presenting institution did not. Conclusions In contemporary treatment of STEMI in Kentucky, ongoing delays to reperfusion therapy remain regardless of treatment strategy. For further improvement in care, acceptance of transfer delays is necessary and institutions should adopt standardized protocols in association with a regional system of care. PMID:23820318

  12. The inhibitory effects and induction of fibrinolytic protein from Eupolyphaga sinensis on the apoptosis of tumer S180 and H22 cells in mice%地鳖纤溶活性蛋白对S180和H22荷瘤小鼠肿瘤的抑制及诱导细胞凋亡作用

    Institute of Scientific and Technical Information of China (English)

    韩雅莉; 曹付春; 余磊; 叶韵; 张卫丽; 黄明星; 刘浩; 黎子蔚

    2011-01-01

    Aim To study the induction effects of fibrinolytic proteins isolated from Eupolyphaga sinensis ( EFT ) on the apoptosis of tumer S180 and H22 cells in mice and its mechanisms. Methods The molecularweight of EFP was detected by sulfate-polyacrylamide gel electrophoresis analysis. The fibrin plate assay was used to test the fibrinolytic activity of EFP. Tumor bearing mouse models ( solid and ascites tumors of S180 and H22 ) were constructed by injecting tumor cells. Cell apoptotic rate was detected by flow cytometry following annexin-V/PI staining. Caspase-3 levels of solid tumors S180 and H22 were measured by spectrophotometry. Effects of EFP on telomerase activity of H22 and S180 ascites tumor cells was detected by telomeric repeat amplification protocol ( TRAP )-silver staining. Results EFP had the effects of inducing apoptosis, raising the level of Caspase-3 of solide tumer S180 and H22 . and decreasing the telomerase activity of ascites tumors of S180 and H22. Conclusion EFP can induce cell apoptosis of S180 and H22 cells.%目的 研究地鳖虫纤溶活性蛋白(fibrinolytic proteins of Eupolyphaga sinensis,EFP)对肿瘤细胞凋亡的诱导作用及其机制.方法 采用纤溶平板法检测了EFP纤溶活性;采用流式细胞术检测了EFP高、中、低浓度对小鼠S180和H22实体瘤组织细胞凋亡的诱导作用;采用免疫组织化学法检测了Caspase-3的表达;采用TRAP-PCR银染法检测了腹水瘤组织端粒酶活性.结果 EFP对S180和H22实体瘤有一定的抑瘤作用,对H22和S180腹水瘤细胞端粒酶活性有一定降低作用,可提高Caspase-3表达水平,诱导细胞凋亡.结论 EFP具有诱导S180和H22肿瘤细胞凋亡作用.

  13. Proteomics study of extracellular fibrinolytic proteases from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from Indonesian fermented food

    Science.gov (United States)

    Nur Afifah, Diana; Rustanti, Ninik; Anjani, Gemala; Syah, Dahrul; Yanti; Suhartono, Maggy T.

    2017-02-01

    This paper presents the proteomics study which includes separation, identification and characterization of proteins. The experiment on Indonesian fermented food such as extracellular fibrinolytic protease from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from red oncom and tempeh gembus was conducted. The experimental works comprise the following steps: (1) a combination of one- and two-dimensional electrophoresis analysis, (2) mass spectrometry analysis using MALDI-TOF-MS and (3) investigation using protein database. The result suggested that there were new two protein fractions of B. licheniformis RO3 and three protein fractions of B. pumilus 2.g. These result has not been previously reported.

  14. Data of evolutionary structure change: 1J3FA-1RSEA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1J3FA-1RSEA 1J3F 1RSE A A MVLSEGEWQLVLHVWAKVEADVAGHGQDILIRLFKSHPE...TLEKFDRFKHLKTEAEMKASEDLKKHGVTVLTGLGAILKKKGHHEAELKPLAQSHAT--KIPIKYLEFISEAIIHVLHSRHPGDFGADAQGAMNKALELFRKDIAAKY...pdbChain>A 1RSEA QDHATKHKIPIK

  15. Colour schemes and façade work at Drottningholm and Stockholm palaces

    OpenAIRE

    2008-01-01

    Jan Lisinski présente les couleurs des façades des palais royaux de Drottningholm et de Stockholm. Les deux palais furent construits au xviie siècle mais firent l’objet de modifications par la suite. La présentation commence par un bref rappel historique centré sur les différentes couleurs utilisées pour les façades puis aborde la façon dont doivent être traitées les façades aujourd’hui. Il sera question en particulier du traitement des pierres, des plâtres, des maçonneries, des aciers et des...

  16. Data of evolutionary structure change: 1B0FA-1O37A [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available -GYDPV >EEE - > ATOM 556 CA ARG ...ntryIDChain>1O37A KSIVHPSYNSN >EEEEE ...> 1B0FA LGRNR-GIASV > - ...KSSGTSYPDV > > ATOM 1849 CA THR ...tryIDChain> AHCYK----SGIQV >GGG ---- EEE>

  17. Data of evolutionary structure change: 1ANFA-3F5FA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1ANFA-3F5FA 1ANF 3F5F A A KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEH...TGKKSHLRKTTEKKLPTAATIAKLQQSEIWKMENEFYEFALEQFQFVRAHAVREKDGELYILAQNFFYEKIYPK...HHHHHHHHHHHHH EEEEE EEE EVID> 0 1ANF A 1ANFA 3.7251410484313965 EVID>EVID> 1 <

  18. Gene polymorphisms of fibrinolytic enzymes in coal workers' pneumoconiosis

    Energy Technology Data Exchange (ETDEWEB)

    Chang, L.C.; Tseng, J.C.; Hua, C.C.; Liu, Y.C.; Shieh, W.B.; Wu, H.P. [Chang Gung Memorial Hospital, Chilung (Taiwan)

    2006-03-15

    The authors assessed the gene polymorphisms of missense C/T polymorphism in exon 6 of the urokinase-plasminogen activator (PLAU) gene (PLAU P141L), A/u-repeat in intron 8 of the tissue-type plasminogen activator (PLAT) gene (PLAT TPA25 Alu insertion), and 4G/5G in the promoter region of the serine proteinase inhibitor, clade E (SERPINE) or plasminogen activator inhibitor type 1 gene (SERPINE1 -675 4G/5G) in 153 healthy volunteers and 154 retired coal miners with coal miners' pneumoconiosis (CWP). The CWP subjects included 94 individuals with simple pneumoconiosis and 60 individuals with progressive massive fibrosis presenting with worse pulmonary function. The distributions of genotypes of these three genes did not differ between the control and CWP subjects or between subjects with simple pneumoconiosis and those with progressive massive fibrosis. However, by assessing duration of work and its interaction with genotypes by means of logistic regression, the authors found the missense C/T polymorphism in exon 6 of the PLAU gene to be an effect modifier of the association between work duration and the development of progressive massive fibrosis.

  19. Transonic Free-To-Roll Analysis of the F/A-18E and F-35 Configurations

    Science.gov (United States)

    Owens, D. Bruce; McConnell, Jeffrey K.; Brandon, Jay M.; Hall, Robert M.

    2004-01-01

    The free-to-roll technique is used as a tool for predicting areas of uncommanded lateral motions. Recently, the NASA/Navy/Air Force Abrupt Wing Stall Program extended the use of this technique to the transonic speed regime. Using this technique, this paper evaluates various wing configurations on the pre-production F/A-18E aircraft and the Joint Strike Fighter (F-35) aircraft. The configurations investigated include leading and trailing edge flap deflections, fences, leading edge flap gap seals, and vortex generators. These tests were conducted in the NASA Langley 16-Foot Transonic Tunnel. The analysis used a modification of a figure-of-merit developed during the Abrupt Wing Stall Program to discern configuration effects. The results showed how the figure-of-merit can be used to schedule wing flap deflections to avoid areas of uncommanded lateral motion. The analysis also used both static and dynamic wind tunnel data to provide insight into the uncommanded lateral behavior. The dynamic data was extracted from the time history data using parameter identification techniques. In general, modifications to the pre-production F/A-18E resulted in shifts in angle-of-attack where uncommanded lateral activity occurred. Sealing the gap between the inboard and outboard leading-edge flaps on the Navy version of the F-35 eliminated uncommanded lateral activity or delayed the activity to a higher angle-of-attack.

  20. The influence of ventilated façade on sound insulation properties of envelope walls

    OpenAIRE

    2017-01-01

    Presented article deals with sound insulation properties of timber structures’ envelope walls. Particularly, the influence of heavy board ventilated façade on laboratory airborne sound insulation R and Rw in dB was studied. The installation method and gaps between façade boards can cause building defects originating in overrating the influence of ventilated cladding on envelope wall acoustic parameters. Real constructions were built for the experimental purposes and measurements, one with gap...

  1. Detection of the synthetic drug 4-fluoroamphetamine (4-FA) in serum and urine.

    Science.gov (United States)

    Röhrich, J; Becker, J; Kaufmann, T; Zörntlein, S; Urban, R

    2012-02-10

    4-Fluoroamphetamine (4-FA) was detected in the blood and urine of two individuals suspected for driving under the influence (DUI). The test for amphetamines in urine subjected to immunoassay screening using the CEDIA DAU assay proved positive. Further investigations revealed a 4-FA cross-reactivity of about 6% in the CEDIA amphetamine assay. 4-FA was qualitatively detected in a general unknown screening for drugs using GC/MS in full scan mode. No other drugs or fluorinated phenethylamines were detected. A validated GC/MS method was established in SIM mode for serum analysis of 4-FA with a limit of detection (LOD) of 1 ng/mL and a lower limit of quantification (LLOQ) of 5 ng/mL. Intra-assay precision was approx. 4% and inter-assay precision approx. 8%. Applying this method, the 4-FA serum concentrations of the two subjects were determined to be 350 ng/mL and 475 ng/mL, respectively. Given the pharmacological data of amphetamine, 4-FA psychoactive effects are to be expected at these serum levels. Both subjects exhibited sympathomimetic effects and psychostimulant-like impairment accordingly.

  2. Architectural Kansei of ‘Wall’ in The Façade Design by Le Corbusier

    Science.gov (United States)

    Sendai, Shoichiro

    The purpose of this paper is to discuss the modern architect Le Corbusier's architectural Kansei (sensibility) on wall in site environment through the analysis of his façade design, using Œuvres complètes (1910-1965, 8 vols., Les éditions d'architecture, Artemis, Zurich) and Le Corbusier Archives (1982-1984, 32 vols., Garland Publishing, Inc. and Fondation Le Corbusier, New York, London, Paris). At first, I arrange five façade types, according to the explanation by Le Corbusier ; ‘fenêtre en longueur (strip window)’, ‘pan de verre (glass wall)’, ‘brise-soleil (sun-breaker)’, ‘loggia’ and ‘claustra’. Through the analysis of the relationship between these types and the design process of each building, we find that Le Corbusier's façade design includes the affirmation and the negation of the ‘wall’ at the same time. In fact, the nature of façade modification during design process is divers: increase in transparency, decrease in transparency and spatialization of façade. That means, Le Corbusier studied the environmental condition by these façade types, and tried to realize the phenomenal openness. This trial bases on the function of architectural Kansei as correspondence between body and environment beyond the physical design.

  3. A BPMN-based process map for the design and construction of façades

    Directory of Open Access Journals (Sweden)

    Eleanor Voss

    2013-12-01

    Full Text Available Corresponding author: Eleanor Voss, Glass and Façade Technology Research Group, Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, UK. E-mail: ev236@cam.ac.uk Process mapping can lead to significant efficiency and quality improvements in construction engineering and is an ideal basis for developing IT support tools. The increasing complexity and multidisciplinary nature of façade design and construction suggest that a process map would be beneficial in this sector of the construction industry, but it has received limited attention to date. This paper presents a verified process map of the façade design and construction process. The map is the first of its kind to represent, in detail, the whole process relevant to all façade types, from commencement of the façade consultant's and contactor's participation, to the end of their involvement. The paper describes the process by which the mapping notation was selected, followed by the development and verification of the process map, including testing in two independent research projects. The BuildingSMART's BPMN notation is found to have superior system features and comprehensibility for this application and the resulting process map is easy to interpret and verify by industry experts. The trialling of the map in the two research projects indicate that the map is a useful tool for assessing process improvements in the façades sector.

  4. Ectopic Expression of FaDREB2 Enhances Osmotic Tolerance in Paper Mulberry

    Institute of Scientific and Technical Information of China (English)

    Mei-Ru Li; Yan Li; Hong-Qing Li; Guo-Jiang Wu

    2011-01-01

    Dehydration-responsive element binding (DREB) proteins are a subfamily of AP2/ERF transcription factors that have been shown to improve tolerance to osmotic stresses in plants.To improve the osmotic stress tolerance of paper mulberry (Broussonetia papyrifera L.Vent),an economically important tree,we transformed it with a plasmid carrying tall fescue (Festuca arundinacea Schreb) FaDREB2 under the control of CaMV 35S.The ectopic expression of FaDREB2 did not cause growth retardation,and the paper mulberry seedlings expressing FaDREB2 showed higher salt and drought tolerance than wild-type plants (WT).After 13 d of withholding water,or 15 d in the presence of 250 mM NaCI,all the WT plants died,while the plants expressing FaDREB2 survived.The FaDREB2 transgenic plants had higher leaf water and chlorophyll contents,accumulated more proline and soluble sugars,and had less membrane damage than the WT plants under high salt and water-deficient conditions.Taken together,the results indicate the feasibility of improving tolerance to multiple environmental stresses in paper mulberry seedlings via genetic engineering,by introducing FaDREB2,which promotes the increased accumulation of osmolytes (soluble sugars and proline),to counter osmotic stresses caused by abiotic factors.

  5. Mechanical stability and fibrinolytic resistance of clots containing fibrin, DNA, and histones.

    Science.gov (United States)

    Longstaff, Colin; Varjú, Imre; Sótonyi, Péter; Szabó, László; Krumrey, Michael; Hoell, Armin; Bóta, Attila; Varga, Zoltán; Komorowicz, Erzsébet; Kolev, Krasimir

    2013-03-08

    Neutrophil extracellular traps are networks of DNA and associated proteins produced by nucleosome release from activated neutrophils in response to infection stimuli and have recently been identified as key mediators between innate immunity, inflammation, and hemostasis. The interaction of DNA and histones with a number of hemostatic factors has been shown to promote clotting and is associated with increased thrombosis, but little is known about the effects of DNA and histones on the regulation of fibrin stability and fibrinolysis. Here we demonstrate that the addition of histone-DNA complexes to fibrin results in thicker fibers (increase in median diameter from 84 to 123 nm according to scanning electron microscopy data) accompanied by improved stability and rigidity (the critical shear stress causing loss of fibrin viscosity increases from 150 to 376 Pa whereas the storage modulus of the gel increases from 62 to 82 pascals according to oscillation rheometric data). The effects of DNA and histones alone are subtle and suggest that histones affect clot structure whereas DNA changes the way clots are lysed. The combination of histones + DNA significantly prolongs clot lysis. Isothermal titration and confocal microscopy studies suggest that histones and DNA bind large fibrin degradation products with 191 and 136 nM dissociation constants, respectively, interactions that inhibit clot lysis. Heparin, which is known to interfere with the formation of neutrophil extracellular traps, appears to prolong lysis time at a concentration favoring ternary histone-DNA-heparin complex formation, and DNase effectively promotes clot lysis in combination with tissue plasminogen activator.

  6. Mechanical Stability and Fibrinolytic Resistance of Clots Containing Fibrin, DNA, and Histones*

    Science.gov (United States)

    Longstaff, Colin; Varjú, Imre; Sótonyi, Péter; Szabó, László; Krumrey, Michael; Hoell, Armin; Bóta, Attila; Varga, Zoltán; Komorowicz, Erzsébet; Kolev, Krasimir

    2013-01-01

    Neutrophil extracellular traps are networks of DNA and associated proteins produced by nucleosome release from activated neutrophils in response to infection stimuli and have recently been identified as key mediators between innate immunity, inflammation, and hemostasis. The interaction of DNA and histones with a number of hemostatic factors has been shown to promote clotting and is associated with increased thrombosis, but little is known about the effects of DNA and histones on the regulation of fibrin stability and fibrinolysis. Here we demonstrate that the addition of histone-DNA complexes to fibrin results in thicker fibers (increase in median diameter from 84 to 123 nm according to scanning electron microscopy data) accompanied by improved stability and rigidity (the critical shear stress causing loss of fibrin viscosity increases from 150 to 376 Pa whereas the storage modulus of the gel increases from 62 to 82 pascals according to oscillation rheometric data). The effects of DNA and histones alone are subtle and suggest that histones affect clot structure whereas DNA changes the way clots are lysed. The combination of histones + DNA significantly prolongs clot lysis. Isothermal titration and confocal microscopy studies suggest that histones and DNA bind large fibrin degradation products with 191 and 136 nm dissociation constants, respectively, interactions that inhibit clot lysis. Heparin, which is known to interfere with the formation of neutrophil extracellular traps, appears to prolong lysis time at a concentration favoring ternary histone-DNA-heparin complex formation, and DNase effectively promotes clot lysis in combination with tissue plasminogen activator. PMID:23293023

  7. Delta-like 1/fetal antigen 1(DLK1/FA1) inhibits BMP2 induced osteoblast differentiation through modulation of NFκB signaling pathway

    DEFF Research Database (Denmark)

    Qiu, Weimin; Abdallah, Basem; Kassem, Moustapha

    as assessed by reduced Alp activity and osteogenic gene expression including Alp, Col1a1, Runx2 and Bglap. In addition, DLK1/FA1 inhibited BMP signaling as demonstrated by reduced gene expression of BMP-responsive genes: Junb and Id1, reduced BMP2 induced luciferase activity in C2C12 BMP luciferase reporter....... Besides, we observed that DLK1/FA1 induced strong NFκB activity evidenced by NFκB responsive luciferase reporter assay and real-time RT-PCR analysis of NFκB target genes. The inhibitory effect of NFκB signaling on BMP signaling was confirmed by luciferase assay in C2C12 BMP luciferase reporter cells...

  8. Coagulation and Fibrinolytic System Protein Profiles in Women with Normal Pregnancies and Pregnancies Complicated by Hypertension.

    Science.gov (United States)

    Hale, Sarah A; Sobel, Burton; Benvenuto, Anna; Schonberg, Adrienne; Badger, Gary J; Bernstein, Ira M

    2012-04-01

    OBJECTIVE: The current study longitudinally evaluated concentrations of fibrinogen (Fib), D-Dimer, plasminogen activator type-1 (PAI-1) and tissue type plasminogen activator (T-Pa) before pregnancy and in the first and third trimesters of pregnancy with a focus on the pregnancy transition. STUDY DESIGN: Twenty healthy, nonsmoking, nulliparous women, aged 29.8 ± 3.0 years, BMI 23.3 ± 3.2 kg/m(2) were studied during menstrual cycle day 8 ± 4 and again in early (11 - 15 wks) and late (31 - 34 wks) pregnancy. Seventeen women had singleton conceptions and delivered at term with uncomplicated pregnancies (CTL) and three women developed complicated hypertension (CH) during pregnancy after the third trimester (late pregnancy) evaluation. Data are means ± SEM, Significance was based on p < 0.05. RESULTS: Fib was the only protein evaluated that increased in early pregnancy relative to the prepregnancy assessment. D-dimer, PAI-1 and T-Pa increased in the third trimester compared with prepregnant and early pregnant values (p < .001). T-PA was significantly higher during late pregnancy in CH subjects compared with CTL (8.1 ± 0.7 ng/ml vs. 5.0 ± 0.2 ng/ml, p = .02). There were no other differences between groups. CONCLUSIONS: Increases in fibrinogen are evident in early pregnancy whereas fibrinolysis, perhaps in response to the procoagulant environment of pregnancy, is increased during late pregnancy. Before development of clinically overt hypertension, T-Pa is increased without concomitant changes in other proteins assessed. This is consistent with altered endothelial function with preeclampsia that may contribute to, or reflect, the vasculopathy accompanying this disorder.

  9. Achieving the nitrite pathway using FA inhibition and process control in UASB-SBR system removing nitrogen from landfill leachate

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    An up-flow sludge blanket(UASB) and sequencing batch reactor(SBR) system was introduced to remove organics and nitrogen from landfill leachate.The synergetic effect of free ammonia(FA) inhibition and process control was used to achieve the nitrite pathway in the SBR.In previous research,inhibition of FA on nitrite oxidizing bacteria(NOB) activity has been revealed and the process control parameters(DO,ORP and pH) exactly indicate the end-point of nitritation.The method was implemented in the SBR achieving stable nitrogen removal via the nitrite pathway from landfill leachate.The degree of nitrite accumulation during the nitritation was monitored along with the simultaneous and advanced removal of organics and nitrogen in the UASB-SBR system.The nitrifying bacteria community was quantitatively analyzed by fluorescence in situ hybridization(FISH) techniques.Batch tests were carried out to investigate the denitritation kinetics of microbial bacteria in the SBR.Experimental results showed that the nitrite pathway could be repeatedly and reliably achieved by synergetic effect of FA inhibition and process control.FISH analysis showed the dominant nitrifying bacteria were ammonia-oxidizing β-Proteobacteria. Relationship between nitrite concentration and nitrite reduction rate followed the Monod-type equation.The maximum specific nitrite utilization rate(k) and half-velocity constant(Ks) were calculated as 0.44 gN gVSS-1d-1and 15.8 mg L-1,respectively.

  10. Purification of c-phycocyanin from Spirulina fusiformis and its effect on the induction of urokinase-type plasminogen activator from calf pulmonary endothelial cells.

    Science.gov (United States)

    Madhyastha, H K; Radha, K S; Sugiki, M; Omura, S; Maruyama, M

    2006-09-01

    c-Phycocyanin (c-pc), a blue coloured, fluorescent protein was purified from blue-green alga, Spirulina fusiformis and its effect on fibrinolytic system in vascular endothelial cells was investigated. The c-pc consisted of two subunits, alpha and beta, whose molecular masses were 16 and 17 kDa, respectively. N-terminal sequences of both subunits were well conserved compared with other blue green algal phycobiliproteins. Fibrinolytic activity in the medium conditioned by calf pulmonary arterial endothelial cells was measured by the fibrin plate method. The c-pc increased the fibrinolytic activity in dose- and time-dependent manners. Fibrin zymographic studies indicated that c-pc-induced urokinase-type plasminogen activator in the cells. These in vitro results suggest that c-pc from S. fusiformis is a potent profibrinolytic protein in the vascular endothelial system.

  11. The cathepsin B inhibitor, z-FA-CMK is toxic and readily induced cell death in human T lymphocytes

    Energy Technology Data Exchange (ETDEWEB)

    Liow, K.Y.; Chow, S.C., E-mail: chow.sek.chuen@monash.edu

    2013-11-01

    The cathepsin B inhibitor, benzyloxycarbonyl-phenylalanine-alanine-chloromethylketone (z-FA-CMK) was found to be toxic and readily induced cell death in the human T cell line, Jurkat, whereas two other analogs benzyloxycarbonyl-phenylalanine-alanine-fluoromethylketone (z-FA-FMK) and benzyloxycarbonyl-phenylalanine-alanine-diazomethylketone (z-FA-DMK) were not toxic. The toxicity of z-FA-CMK requires not only the CMK group, but also the presence of alanine in the P1 position and the benzyloxycarbonyl group at the N-terminal. Dose–response studies showed that lower concentrations of z-FA-CMK induced apoptosis in Jurkat T cells whereas higher concentrations induced necrosis. In z-FA-CMK-induced apoptosis, both initiator caspases (-8 and -9) and effector caspases (-3, -6 and -7) were processed to their respective subunits in Jurkat T cells. However, only the pro-form of the initiator caspases were reduced in z-FA-CMK-induced necrosis and no respective subunits were apparent. The caspase inihibitor benzyloxycarbonyl-valine-alanine-aspartic acid-(O-methyl)-fluoromehylketone (z-VAD-FMK) inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentration of z-FA-CMK but has no effect on z-FA-CMK-induced necrosis and the loss of initiator caspases. This suggests that the loss of initiator caspases in Jurkat T cells during z-FA-CMK-induced necrosis is not a caspase-dependent process. Taken together, we have demonstrated that z-FA-CMK is toxic to Jurkat T cells and induces apoptosis at low concentrations, while at higher concentrations the cells die of necrosis. - Highlights: • z-FA-CMK is toxic and induce cell death in the human T cells. • z-FA-CMK toxicity requires the CMK group, alanine and the benzyloxycarbonyl group. • z-FA-CMK induced apoptosis at low concentration and necrosis at high concentration.

  12. Novel bioactive Co-based alloy/FA nanocomposite for dental applications

    Directory of Open Access Journals (Sweden)

    Mohammadhossein Fathi

    2012-01-01

    Full Text Available Background: Dental cobalt base alloys are biocompatible dental materials and have been widely used in dentistry. However, metals are bioinert and may not present bioactivity in human body. Bioactivity is the especial ability to interact with human body and make a bonding to soft and hard tissues. The aim of the present research was fabrication and bioactivity evaluation of novel cobalt alloy/Fluorapatite nanocomposite (CoA/FaNC with different amounts of Fluorapatite (FA nanopowder. Materials and Methods: Co-Cr-Mo alloy (ASTM F75 powder was prepared and mixed in a planetary ball mill with different amounts of FA nanopowders (10, 15, 20% wt. Prepared composite powders were cold pressed and sintered at 1100°C for 4 h. X-ray diffraction (XRD, scanning electron microscopy and transition electron microscopy techniques were used for phase analysis, crystallite size determination of FA and also for phase analysis and evaluation of particle distribution of composites. Bioactivity behavior of prepared nanocomposites was evaluated in simulated body fluid (SBF for 1 up to 28 days. Results: Results showed that nucleus of apatite were formed on the surface of the prepared CoA/FaNC during 1 up to 28 days immersion in the SBF solution. On the other hand, CoA/FaNC unlike Co-base alloy possessed bone-like apatite-formation ability. Conclusion: It was concluded that bioinert Co-Cr-Mo alloy could be successfully converted into bioactive nanocomposite by adding 10, 15, 20 wt% of FA nano particles.

  13. New methods for testing fire resistance of wood façade systems

    Directory of Open Access Journals (Sweden)

    Mårtensson August

    2016-01-01

    Full Text Available Arson in schools has been a huge problem in Sweden over the last fifteen years. The average amount of school arsons between 2000 and 2014 was 285 cases each year which corresponds to 50% of the total amount of reported fires in school buildings. This is a well-known problem and a lot of research has been done in this area. Investigations has been done about fire and heat detection systems, different technical factors significance in fire scenarios and how to prevent adolescents from starting fires. Another part of the problem that partly been investigated is how the schools are constructed. Roughly 50% of the arsons are outside of the school building. In Sweden one and two storey buildings are allowed to be built with wooden façades in accordance with the building code, which is one of the reasons many schools are built with wooden façade systems. The most critical part in a wood façade system from a fire safety perspective is concluded to be the eaves because of how they usually are built to let air pass through. Even though a wood façade isn't as well resistant to fire compared to a concrete façade, three versions of new test methods for combustible façades have been developed to make it possible to make sure in advance that a construction is resistant enough. The new test methods are focused on specific details and parts of a façade system to provide a more informative and useful result compared to SP Fire 105. Observations and measurements of flame spread and temperature changes in the eave, over the window joints and in the air gap are made. With these parameters in consideration criteria's has been chosen for a critical temperature of 280 ∘C at a critical time of 20 minutes.

  14. Dietary t10,c12-CLA but not c9,t11 CLA reduces adipocyte size in the absence of changes in the adipose renin-angiotensin system in fa/fa Zucker rats.

    Science.gov (United States)

    DeClercq, Vanessa; Zahradka, Peter; Taylor, Carla G

    2010-11-01

    In obesity, increased activity of the local renin-angiotensin system (RAS) and enlarged adipocytes with altered adipokine production are linked to the development of obesity-related health problems and cardiovascular disease. Mixtures of conjugated linoleic acid (CLA) isomers have been shown to reduce adipocyte size and alter the production of adipokines. The objective of this study was to investigate the effects of feeding individual CLA isomers on adipocyte size and adipokines associated with the local adipose RAS. Male fa/fa Zucker rats received either (a) control, (b) cis(c)9,trans(t)11-CLA, or (c) t10,c12-CLA diet for 8 weeks. The t10,c12-CLA isomer reduced adipocyte size and increased cell number in epididymal adipose tissue. RT-PCR and Western blot analysis revealed that neither CLA isomer altered mRNA or protein levels of angiotensinogen or AngII receptors in adipose tissue. Likewise, levels of the pro-inflammatory cytokines TNF-α and IL-6 or the anti-inflammatory cytokine IL-10 were unchanged in adipose tissue. Similarly, neither CLA isomer had any effect on phosphorylation nor DNA binding of NF-κB. Our results suggest that although the t10,c12-CLA isomer had beneficial effects on reducing adipocyte size in obese rats, this did not translate into changes in the local adipose RAS or associated adipokines.

  15. Regulation of p53-mediated changes in the uPA-fibrinolytic system and in lung injury by loss of surfactant protein-C expression in alveolar epithelial cells.

    Science.gov (United States)

    Puthusseri, Bijesh; Marudamuthu, Amarnath S; Tiwari, Nivedita; Fu, Jian; Idell, Steven; Shetty, Sreerama

    2017-04-06

    Pulmonary surfactant protein-C (SP-C) expression by type II alveolar epithelial cells (AECs) is markedly reduced in diverse types of lung injuries and is often associated with AEC apoptosis. It is unclear whether loss of SP-C contributes to the increased p53 and urokinase-type plasminogen activator (uPA) system cross talk and apoptosis of AECs. We therefore inhibited SP-C expression in human and murine AECs using lentivirus vector expressing shRNA and tested p53 and downstream changes in uPA-fibrinolytic system. Inhibition of SP-C expression in AECs induced p53 and activated caspase-3, indicating AEC apoptosis. We also found that bleomycin or cigarette smoke exposure failed to inhibit SP-C expression or apoptosis in AECs in p53- and plasminogen activator inhibitor-1 (PAI-1)-deficient mice. Depletion of SP-C expression by lentiviral SP-C shRNA in PAI-1-deficient mice failed to induce p53 or apoptosis in AECs, while it increased both AEC p53 and apoptosis in wild type or uPA-deficient mice. SP-C inhibition in AECs also increased in CXCL1 and CXCL2, and their receptor CXCR2 as well as ICAM-1 expression, indicative of a pro-inflammatory response. Overexpression of p53-binding 3'UTR sequences in AECs inhibited PAI-1 induction while maintaining uPA and uPAR protein and mRNA expression. Further, caveolin-1 expression and phosphorylation were increased in AECs indicating an intricate link between caveolin-1 and Src kinase-mediated cell signalling and AEC apoptosis due to loss of SP-C expression through p53 and uPA system-mediated cross-talk. The role of uPA, PAI-1 and p53 in the regulation of AEC apoptosis after injury was also determined in knock out mice.

  16. A BPMN-Based Process Map for the Design and Construction of Façades

    Directory of Open Access Journals (Sweden)

    Eleanor Voss

    2013-12-01

    Full Text Available Process mapping can lead to significant efficiency and quality improvements in construction engineering and is an ideal basis for developing IT support tools. The increasing complexity and multidisciplinary nature of façade design and construction suggest that a process map would be beneficial in this sector of the construction industry, but it has received limited attention to date. This paper presents a verified process map of the façade design and construction process.  The map is the first of its kind to represent, in detail, the whole process relevant to all façade types, from commencement of the façade consultant’s and contactor’s participation, to the end of their involvement. The paper describes the process by which the mapping notation was selected, followed by the development and verification of the process map, including testing in two independent research projects. The BuildingSMART’s BPMN notation is found to have superior system features and comprehensibility for this application and the resulting process map is easy to interpret and verify by industry experts. The trialling of the map in the two research projects indicate that the map is a useful tool for assessing process improvements in the façades sector.

  17. Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent.

    Science.gov (United States)

    Jeppesen, J; Albers, P H; Rose, A J; Birk, J B; Schjerling, P; Dzamko, N; Steinberg, G R; Kiens, B

    2011-04-01

    The aim of this study was to investigate the molecular mechanisms regulating FA translocase CD36 (FAT/CD36) translocation and FA uptake in skeletal muscle during contractions. In one model, wild-type (WT) and AMP-dependent protein kinase kinase dead (AMPK KD) mice were exercised or extensor digitorum longus (EDL) and soleus (SOL) muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and FA uptake in response to muscle contractions were investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, 5-aminoimidazole-4-carboxamide ribonucleoside only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions was associated with increased FA uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and FA uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.

  18. Evaluación del efecto de niacina en un modelo de obesidad con sindrome metábolico en ratas Zucker-Zucker (fa/fa) longevas

    OpenAIRE

    Ana Laura Esquivel Campos; María Cristina Fresán Orozco; Miguel Mosqueda Cabrera; Julia Pérez Ramos

    2012-01-01

    Se evaluó el efecto farmacológico de la niacina sobre los niveles plasmáticos de adiponectina, leptina, IL-6 y TNF-α, así como el intercambio gaseoso, en un modelo de obesidad-vejez con hiperglicemia y dislipidemia en ratas macho de la cepa Zucker-Zucker (fa/fa) La niacina disminuyó significativamente los niveles de colesterol y triglicéridos. Mejoró el intercambio gaseoso, incrementó la presión parcial arterial de oxígeno, y disminuyó la presión parcial de dióxido de carbono y redujo los niv...

  19. Production planning support system using. Phi. NET in FA. FA bun prime ya ni okeru. Phi. NET oyo seisan keikau shien system

    Energy Technology Data Exchange (ETDEWEB)

    Igawa, Y.; Hibino, K. (Fuji Electric Co. Ltd., Tokyo (Japan))

    1991-08-10

    In response to a wide use of the production simulation for production scheduling in the domain of factory automation (FA), a system of a machining flexible manufacturing system (FMS) production line using a general-purpose distribution control system building tool {Phi}NET was developed by Fuji Electric. In this report, an outline of this system was introduced as to its constitution and performance. This FMS production line has functions of the production scheduling, the production simulation, and the production instruction. Among them, the {Phi}NET model, in which the network model called petri-net and the production rule were combined, was applied to the production simulation. Consequently, was provided a prospect that the {Phi}NET would be developed to an FA simulator with which a production engineer can build a model with his own field language and conduct a simulation. 4 figs.

  20. FoxO3a mediates transforming growth factor-beta1-induced apoptosis in FaO rat hepatoma cells.

    Science.gov (United States)

    Kim, Byung-Chul

    2008-10-31

    FoxO3a is a member of the forkhead box class O (FoxO) transcription factor family and an important regulator of apoptosis. This work aimed to elucidate the involvement of FoxO3a in transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in FaO rat hepatoma cells. TGF-beta1 caused a time-dependent activation of FoxO3a and a subsequent increase in FoxO response-element-containing luciferase reporter activity, which was Akt-sensitive. The FaO cells stably transfected with a wild type FoxO3a were more susceptible to the formation of apoptotic bodies, populations of sub-G1 apoptotic cells, and collapse of the mitochondrial-membrane potential triggered by TGF-beta1. In contrast, transfection with small-interfering RNA (siRNA) oligonucleotide specific for FoxO3a significantly inhibited caspase activation in FaO cells treated with TGF-beta1. It thus appears that FoxO3a plays a crucial mediatory role in the TGF-beta1 signaling pathway leading to apoptosis.

  1. The new self-supporting face brick façades

    Directory of Open Access Journals (Sweden)

    C. del Río

    2016-12-01

    Full Text Available The ‘Self-Supporting’ face brick façade is the result of a profound reflection on the different façade solutions that have been used throughout the last century for the pillar-structured buildings, and is based on the utilization of the potential that ceramic materials have when they work in compression. The construction solution consists in placing the entire outer sheet of the enclosure ahead of the building’s structure, so as to use the brick wall itself as its own supporting structure. This construction type eliminates the thermal bridges at façade-slab joints, and is more effective regarding mechanical behaviour than conventional solutions, since the own-weight effect contributes beneficially to stability against horizontal actions.

  2. [Data mining analysis of professor Li Fa-zhi AIDS itchy skin medical record].

    Science.gov (United States)

    Wang, Dan-Ni; Li, Zhen; Xu, Li-Ran; Guo, Hui-Jun

    2013-08-01

    Analysis of professor Li Fa-zhi in the treatment of AIDS drug laws of itchy skin, provide the corresponding drug reference basis for Chinese medicine treatment of AIDS, skin itching. By using the method of analyzing the complex network of Weishi county, Henan in 2007 October to 2011 July during an interview with professor Li Fa-zhi treatment of AIDS patients with skin pruritus, etiology and pathogenesis analysis, skin itching AIDS syndrome differentiation of old Chinese medicine treatment and medication rule. The use of multi-dimensional query analysis, core drug skin itching AIDS treatment in this study as a windbreak, cicada slough, bupleurum, Qufeng solution table drug, licorice detoxification efficacy of drugs, Radix Scutellariae, Kochia scoparia, clearing away heat and promoting diuresis medicine; core prescription for Jingfang San streak virus. Professor Li Fa-zhi treatment of AIDS in the skin itching Qufeng solution table dehumidification antipruritic treatment.

  3. Numerical simulations of the ISO 13785-2 façade fire tests

    Directory of Open Access Journals (Sweden)

    Hostikka Simo

    2016-01-01

    Full Text Available In this work we created a numerical model of the ISO 13785-2 test setup for testing the fire behaviour of building façade systems, and used the model to simulate the thermal environment on the façade. The model, created using Fire Dynamics Simulator –software, was first validated using the experimental data by Yoshioka et al.(2012. Next, the sensitivity of the façade heat fluxes on the geometrical and model parameters was studied, revealing for instance that the size of the combustion chamber window will influence the thermal exposure high above the window. Finally, the model was used to estimate the thickness of non-combustible insulation layer that is needed to protect combustible materials from melting or decomposition.

  4. The influence of ventilated façade on sound insulation properties of envelope walls

    Directory of Open Access Journals (Sweden)

    Fišarová Zuzana

    2017-01-01

    Full Text Available Presented article deals with sound insulation properties of timber structures’ envelope walls. Particularly, the influence of heavy board ventilated façade on laboratory airborne sound insulation R and Rw in dB was studied. The installation method and gaps between façade boards can cause building defects originating in overrating the influence of ventilated cladding on envelope wall acoustic parameters. Real constructions were built for the experimental purposes and measurements, one with gaps between boards and one with simply eliminated gaps for mutual comparison. The results obtained were processed to make tables and graphs and to derive recommendations for the design of this type of constructions involving the general installation method of façade boards. Detailed results are depicted in conclusions.

  5. Changes in protein expression due to deleterious mutations in the FA/BRCA pathway.

    Science.gov (United States)

    Salles, Daniela; Cabral, Rosa Estela Caseira; Pizzatti, Luciana; Bisch, Paulo M; Paixão, Julio Cesar; de Almeida, Carlos Eduardo Bonacossa; Seuánez, Héctor N; Cabral-Neto, Januario Bispo

    2007-12-28

    Inherited deleterious mutations in one of the Fanconi anemia genes lead to a disease, characterized by bone marrow failure, myeloid leukemia, and hypersensitivity to DNA damage. We identified proteins likely associated to the molecular signaling pathways involved in DNA repair of interstrand cross-link lesions and in mechanisms of genomic stability mediated by FA/BRCA pathways. We compared protein maps resolved by bidimensional electrophoresis and analyzed differentially expressed proteins, by mass spectrometry, between FA complementation group C (FANCC)-deficient cells, and their ectopically corrected counterpart in physiological conditions or after treatment with MMC. We found six differentially expressed proteins; among them, the checkpoint mediator protein MDC1 whose expression was disrupted in FANCC-/- cells. The potential role of differentially expressed proteins in FA phenotype is discussed.

  6. Explication of Definitional Description and Empirical Use of Fraction of Orally Administered Drugs Absorbed From the Intestine (Fa) and Intestinal Availability (Fg): Effect of P-glycoprotein and CYP3A on Fa and Fg.

    Science.gov (United States)

    Tanaka, Yuta; Kitamura, Yoshiaki; Maeda, Kazuya; Sugiyama, Yuichi

    2016-02-01

    Conventionally, it is believed that the fraction of orally administered drugs absorbed from the intestine (Fa) and intestinal availability (Fg) are independently determined by the apical membrane permeation and intestinal metabolism, respectively. However, the validity of this belief has not been well discussed, and Fa and Fg are often used without careful definition. In this review, Fa and Fg are mathematically described based on their definitions under the linear kinetics of metabolism and transport. Even considering with different models, intestinal metabolic enzymes such as cytochrome P450 3A affected both Fa and Fg, whereas apical efflux transporters including P-glycoprotein had no influence on Fg at least under the linear condition. To determine whether Fa and Fg calculated using different clinical methods are identical, empirical Fa and Fg were mathematically described based on "feces method" and "grapefruit juice method" and compared with their definitions. Fa and Fg obtained by the feces method corresponded with their definitions whereas the grapefruit juice method provided smaller Fa and larger Fg particularly for dual substrates of P-glycoprotein and cytochrome P450 3A with low membrane permeability. Our analyses suggest that the definitions and calculation methods of Fa and Fg should be considered when we intend to separately determine these values.

  7. Calculation Method and Analysis for the Annual Power Generation of PV Façades in China

    Institute of Scientific and Technical Information of China (English)

    Shen Xu; ZhongMing Hu; Jian Kang; Wei Liao; Jing Huang

    2014-01-01

    The application of PV façades emerges greatly in recent years and however its calculation methods and analysis remains insufficient under the weather conditions of China. In such demand, this paper investigates PV façade in terms of PV electricity generation in different arrangements and weather conditions of four major cities in China. The calculation models for PV façade are developed and validated by comparing the results with the measured data from the field experiments. A parametric study is carried out to provide a reference for the optimal design of the PV façades. The results show that with various cities, building orientations, building forms, materials and arrangements of PV modules, there is a distinct difference in the electrical output energy of PV façades. Weather conditions play a very important role in terms of PV generation performance of PV façades.

  8. FA1 immunoreactivity in endocrine tumours and during development of the human fetal pancreas; negative correlation with glucagon expression

    DEFF Research Database (Denmark)

    Tornehave, D; Jensen, Charlotte Harken; Teisner, B;

    1996-01-01

    proteins delta and notch and to the murine preadipocyte differentiation factor Pref-1. These proteins participate in determining cell fate choices during differentiation. We now report that FA1 immunoreactivity is present in a number of neuroectodermally derived tumours as well as in pancreatic endocrine...... tumours. A negative correlation between FA1 and glucagon immunoreactants in these tumours prompted a reexamination of FA1 immunoreactants during fetal pancreatic development. At the earliest stages of development, FA1 was expressed by most of the non-endocrine parenchymal cells and, with ensuing...... development, gradually disappeared from these cells and became restricted to insulin-producing beta cells. Throughout development FA1 was not detected in endocrine glucagon, somatostatin or pancreatic polypeptide cells. Moreover, developing insulin cells that coexpressed glucagon were negative for FA1. Thus...

  9. Effects of inhaled plasminogen activator on the balance between coagulation and fibrinolysis in traumatized pigs

    DEFF Research Database (Denmark)

    Münster, A-M B; Rasmussen, L; Sidelmann, Johannes Jakobsen

    2002-01-01

    the effect of severe trauma on the alveolar fibrinolytic/coagulation balance, and the effect here-upon of inhalation of single-chain urokinase plasminogen activator (scu-PA) in pigs. The study shows an increased concentration of scu-PA in the bronchoalveolar lavage fluid of the treated animals in association...

  10. A Security-façade Library for Virtual-observatory Software

    Science.gov (United States)

    Rixon, G.

    2009-09-01

    The security-façade library implements, for Java, IVOA's security standards. It supports the authentication mechanisms for SOAP and REST web-services, the sign-on mechanisms (with MyProxy, AstroGrid Accounts protocol or local credential-caches), the delegation protocol, and RFC3820-enabled HTTPS for Apache Tomcat. Using the façade, a developer who is not a security specialist can easily add access control to a virtual-observatory service and call secured services from an application. The library has been an internal part of AstroGrid software for some time and it is now offered for use by other developers.

  11. El rol de los nuevos anticoagulantes en pacientes con fibrilación auricular (FA)

    OpenAIRE

    Ferrari, Eliseo; Pérez Duhalde, Carolina; Bertaini, Omar; Farina, Juan P

    2016-01-01

    Alrededor de un 15 % de los accidentes cardiovasculares (ACV) son de tipo embolico, consecuencia principalmente de la Fibrilación Auricular (FA)1,2. Los anticoagulantes cumarínicos han demostrado reducir en forma significativa el riesgo de embolia en pacientes con FA 2,3. Las desventajas de estos fármacos son el riesgo de sangrado y que requieren un monitoreo estricto; lo que conlleva una adherencia poco confiable, alrededor de un 30 a 60%, y una extrema variabilidad en sus efectos 4. En a...

  12. 1株纤溶酶菌株的筛选鉴定及提高产酶量的方法研究%Screening and Identification of a Fibrinolytic Enzyme Producing Strain and Research on Increasing its Enzyme Yield

    Institute of Scientific and Technical Information of China (English)

    王海曼; 宋刚; 柴洋洋; 平文祥; 葛菁萍

    2011-01-01

    从齐齐哈尔市拜泉县的自酿农家豆酱制品中分离筛选出1株产纤溶酶菌株HDBF-DJ3,对其进行菌体、菌落、生理生化特征分析,并结合16S rDNA序列分析结果,鉴定其为枯草芽孢杆菌.经亚硝基胍诱变筛选得到突变株HDBF-DJ3N7,其纤溶酶活力为368.78 IU/mL,是出发菌株的2.1倍.对菌株HDBF-DJ3N7进行连续10代的培养,结果表明该突变株的高产纤溶酶特性能够稳定遗传.该菌株有望应用到生产领域.%A flbrinolytic enzyme producing strain named HDBF-DJ3 was isolated from soy-bean paste which was sampled from Baiquan county of Qiqihar district. It was initially identified as Bacillus subtilis by testing its morphological, physiological and biochemical characteristics as well as 16S rDNA sequence analysis. A mutant named HDBF-DJ3N7 was obtained from HDBF-DJ3 by mutagenesis treatment of nitrosoguanidine(NTG). The fibrinolysin activity of HDBF-DJ3N7 was 368.78 U/mL, which was 2.1 times higher than that of HDBF-DJ3. It was indicated that the mutant had genetic stability on fibrinolytic enzyme production by 10 generation cultivation and it could be applied into production field.

  13. Microvascular obstruction after successful fibrinolytic therapy in acute myocardial infarction. Comparison of reteplase vs reteplase+abciximab: A cardiovascular magnetic resonance study

    Directory of Open Access Journals (Sweden)

    Tiziano Gherli

    2010-05-01

    Full Text Available Background. About one third of patients with TIMI 3 after reperfusion have evidence of microvascular obstruction (MO which represents an independent predictor of myocardial wall rupture. This explains all efforts made to prevent MO. Magnetic resonance imaging (MRI has proved to be particularly useful in detecting MO. The aim of this study was to evaluate with MRI if different fibrinolytic regimens in acute myocardial infarction display different effects on left ventricle (LV volumes and ejection fraction (EF, as well as on myocardial infarct size (MIsz and MO. Methods. Twenty male patients, mean age 58 years, affected by acute myocardial infarction, ten anterior and ten inferior, were treated with: full dose reteplase in ten, and half dose reteplase plus full dose abciximab (R+Abcx in the other ten patients. In the fourth day after hospital admission, MRI STIR T2 images were used to quantify MIsz, while 2dflash cineloops were used after the injection of gadolinium, to quantify LV volumes, EF and to detect MO. Results. LV EF was higher in R+Abcx 51±10 than in reteplase 41±8. MIsz was similar in both treatment groups: however a close relationship was present between MIsz and EF in the reteplase group indicating that the greater the MIsz the lower the EF. In R+Abcx this relationship was no longer present, suggesting a protective effect of the drug on microcirculation. In fact extensive MO was present in 25% of all cases, 80% of which in the reteplase group while only 20% in R+Abcx. Conclusion. R+Abcx prevents MO: compared to traditional fibrinolytic therapy it allows better LV function and most likely improved long term survival.

  14. Angiographic and clinical outcomes in elderly subjects treated with percutaneous coronary intervention following fibrinolytic administration for ST-elevation myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    Ajay J. Kirtane; C. Michael Gibson; Adam H. Skolnick; Hilary Oman; Christopher Ruisi; Leida Perez; Nicole Kraimer; Dimitrios Karmpaliotis; Duane S. Pinto; Eugene Braunwald

    2005-01-01

    Background Prior studies have demonstrated that the achievement of faster coronary artery flow following reperfusion therapies is associated with improved outcomes among ST-elevation myocardial infarction (STEMI) patients. The association of patient age with angiographic characteristics of flow and perfusion after rescue/adjunctive percutaneous coronary intervention (PCI) following the administration of fibrinolytic therapy has not been previously investigated. Objectives and Methods We examined the association between age (≥ 70 years or < 70years)and clinical and angiographic outcomes in 1472 STEMI patients who underwent rescue/adjunctive PCI following fibrinolytic therapy in 7 TIMI trials. We hypothesized that elderly patients would have slower post-PCI epicardial flow and worsened outcomes compared to younger patients. Results The 218 patients aged ≥ 70 years (14.8%) had more comorbidities than younger patients. Although these patients had significant angiographic improvement in TIMI frame counts and rates of TIMI Grade 3 flow following rescue/adjunctive PCI, elderly patients had higher (slower)post-PCI TIMI frame counts compared to the younger cohort (25 vs 22 frames, P = 0.039), and less often achieved post-PCI TIMI Grade 3 flow (80.1 vs 86.4%, P = 0.017). The association between age ( ≥70 years) and slower post-PCI flow was independent of gender, time to treatment, left anterior descending (LAD) lesion location, and pulse and blood pressure on admission. Elderly patients also had 4-fold higher mortality at 30 days (12.0 vs 2.7%,P = 0. 001 ). Conclusions This study suggests one possible mechanism underlying worsened outcomes among elderly STEMI patients insofar as advanced chronological age was associated with higher TIMI frame counts and less frequent TIMI Grade 3 flow after rescue/adjunctive PCI.

  15. Trans-10, cis-12-conjugated linoleic acid reduces the hepatic triacylglycerol content and the leptin mRNA level in adipose tissue in obese Zucker fa/fa rats.

    Science.gov (United States)

    Gudbrandsen, Oddrun A; Rodríguez, Enrique; Wergedahl, Hege; Mørk, Sverre; Reseland, Janne E; Skorve, Jon; Palou, Andreu; Berge, Rolf K

    2009-09-01

    Conjugated linoleic acid (CLA) isomers have been reported to reduce body weight and beneficially affect glucose metabolism in animals, but the results are inconsistent and seem to depend on animal model and type of CLA isomer. In the present study, feeding male Zucker fa/fa rats diets supplemented with 1% trans-10, cis-12-CLA for 10 d reduced the liver TAG content without improving the overall adiposity, and enhanced hepatic mitochondrial and peroxisomal beta-oxidation. The increased carnitine palmitoyltransferase (CPT)-I activity and mRNA level as well as the increased n-3:n-6 PUFA ratio in liver suggest that trans-10, cis-12-CLA increased the hepatic beta-oxidation by stimulation of PPARalpha. The reduced hepatic TAG content may be partly due to lower activity of stearoyl-CoA desaturase, as the ratios of 18 : 1n-9:18 : 0 and 16 : 1n-7:16 : 0 were reduced in liver. Trans-10, cis-12-CLA increased the CPT-I mRNA in retroperitoneal white adipose tissue (WAT), and increased uncoupling protein-2 mRNA in epididymal and inguinal WAT depots. Leptin mRNA level was decreased in all examined WAT depots, implying reduced insulin sensitivity. The resistin mRNA level was increased in all WAT depots, whereas adiponectin mRNA was reduced in inguinal and retroperitoneal WAT. The present results suggest that dietary supplementation with trans-10, cis-12-CLA may increase the catabolism of lipids in liver and adipose tissue. Moreover, we provide new data suggesting that trans-10, cis-12-CLA modulates the expression of resistin and adiponectin inversely in adipose tissue. Hence, the present results suggest that trans-10, cis-12-CLA may have some beneficial effects on lipid metabolism and adiposity but possibly reduces insulin sensitivity.

  16. Acquisition and observation logs for seabed video and sediment samples from Stellwagen Bank on U.S. Geological Survey field activity 2015-062-FA, aboard the R/V Auk, Oct. 21 and 22 and Nov. 3 and 4 2015 (PDF files)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This field activity is part of the effort to map geologic substrates of the Stellwagen Bank National Marine Sanctuary region off Boston, Massachusetts. The overall...

  17. Antitoxin activity of Mimosa pudica root extracts against Naja naja and Bangarus caerulus venoms

    Directory of Open Access Journals (Sweden)

    Subramani Meenatchisundaram, Selvin Priyagrace, Ramasamy Vijayaraghavan, Ambikapathi Velmurugan, Govindarajan Parameswari, Antonysamy Michael

    2009-12-01

    Full Text Available Aqueous extract of dried roots of Mimosa pudica was tested for inhibitory activity on lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity of Naja naja and Bangarus caerulus venoms. The aqueous extract displayed a significant inhibitory effect on the lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity. About 0.14 mg and 0.16 mg of M. pudica extracts were able to completely neutralize the lethal activity of 2LD50 of Naja naja and Bangarus caerulus venoms respectively. The present finding suggests that aqueous extract of M. pudica root possesses compounds, which inhibit the activity of Naja naja and Bangarus caerulus venoms.

  18. Antitoxin activity of Mimosa pudica root extracts against Naja naja and Bangarus caerulus venoms

    Directory of Open Access Journals (Sweden)

    Subramani Meenatchisundaram

    2009-06-01

    Full Text Available Aqueous extract of dried roots of Mimosa pudica was tested for inhibitory activity on lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity of Naja naja and Bangarus caerulus venoms. The aqueous extract displayed a significant inhibitory effect on the lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity. About 0.14 mg and 0.16 mg of M. pudica extracts were able to completely neutralize the lethal activity of 2LD50 of Naja naja and Bangarus caerulus venoms respectively. The present finding suggests that aqueous extract of M. pudica root possesses compounds, which inhibit the activity of Naja naja and Bangarus caerulus venoms.

  19. Effects of inbreeding on survival, body weight and fluctuating asymmetry (FA) in Nile tilapia, Oreochromis niloticus

    NARCIS (Netherlands)

    Fessehaye, Y.; Komen, J.; Rezk, M.A.; Arendonk, van J.A.M.; Bovenhuis, H.

    2007-01-01

    The present study investigated the effects of different levels of inbreeding on survival, growth, body weight and fluctuating asymmetry (FA) in O. niloticus. We mated 20 sires and 35 dams in a full-sib/half-sib mating design to produce 35 full-sib families with expected level of inbreeding (F%.) of

  20. HEAT TRANSFER EVALUATION OF HFC-236FA IN CONDENSATION AND EVAPORATION

    Science.gov (United States)

    The report gives results of an evaluation of the shell-side heat transfer performance of hydrofluorocarbon (HFC)-236fa, which is considered to be a potential substitute for chlorofluorocarbon (CFC)-114 in Navy shipboard chillers, for both conventional finned [1024- and 1575-fpm (...

  1. First International Workshop on Human Factors in Modeling (HuFaMo 2015)

    DEFF Research Database (Denmark)

    Störrle, Harald; Chaudron, Michel R. V.; Amaral, Vasco

    2015-01-01

    and systematic approaches to assert usability in modeling and confirm the claims of productivity. This workshop creates a space for discussion being a get together of both MDE, Usability, Human Interfaces and the Experimental Software engineering community. HuFaMo expressly focuses on human factors, in order...

  2. MISCIBILITY, SOLUBILITY, VISCOSITY, AND DENSITY MEASUREMENTS FOR R-236FA WITH POTENTIAL LUBRICANTS

    Science.gov (United States)

    The report gives results of miscibility, solubility, viscosity, and density measurements for refrigerant R-236fa and two potential lubricants . (The data are needed to determine the suitability of refrigerant/lubricant combinations for use in refrigeration systems.) The tested oi...

  3. Foetal antigen 2 (FA2) in the stromal reaction induced by breast carcinoma

    DEFF Research Database (Denmark)

    Rasmussen, H B; Teisner, B; Andersen, J A

    1992-01-01

    An indirect immunoperoxidase technique was used to examine the distribution of foetal antigen 2 (FA2), a recently described basement membrane (BM)-associated antigen, in invasive breast carcinoma (n = 34), fibroadenoma (n = 5) and normal breast tissue (n = 5), and to compare its distribution...

  4. Missionaries and Tonic Sol-fa Music Pedagogy in 19th-Century China

    Science.gov (United States)

    Southcott, Jane E.; Lee, Angela Hao-Chun

    2008-01-01

    In the 19th century, Christian missionaries in China, as elsewhere, used the Tonic Sol-fa method of music instruction to aid their evangelizing. This system was designed to improve congregational singing in churches, Sunday schools and missions. The London Missionary Society and other evangelical groups employed the method. These missionaries took…

  5. Objective and subjective evaluation of façade sound insulation

    DEFF Research Database (Denmark)

    Ordoñez, Rodrigo Pizarro; Visentin, Chiara; Markovic, Milos;

    2013-01-01

    Façade insulation of several different construction types were subjectively evaluated using two psychoacoustic methods: paired comparisons using a two alternative forced choice (2-AFC) paradigm and direct scaling using a visual analogue scale (VAS). The stimuli used in the evaluations were obtained...

  6. Data of evolutionary structure change: 1B0FA-1O3AA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available YDPV >EEE - > ATOM 556 CA ARG A ...ryIDChain>1O3AA KSIVHPSYNSN cture>EEEEE ucture...bChain> 1B0FA LGRNR-GIASV > - ...ence>TKSSGTSYPDV > > ATOM 1849 C...A AHCYK----SGIQV >GGG ---- EEEe>

  7. F.A. FON HAYEK''S AUSTRIAN THEORY OF COMPETITION

    OpenAIRE

    Nesterenko, O.

    2008-01-01

    F.A. fon Hayek's approach to competition's understanding has been clarified, critical analysis directions of neo-classical model of perfect competition have been analyzed. Specific points of Austrian competitive theory have been determined and scientist ideas evolution to competitive process has been worked out.

  8. Data of evolutionary structure change: 1A3FA-2NOTA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1A3FA-2NOTA 1A3F 2NOT A A NLYQFKNMIKCTV-PSRSWWDFADYGCYCGRGGSGTPVD...ignment> 0 2NOT A 2NOTA...in> -65.49500274658203 5.223999977111816 14.175999641418457 ...99998712539673 0.8920000195503235 tion> 1.2738020420074463 2.701137065887451 ...

  9. Consumption of fa cai Nostoc soup: a potential for BMAA exposure from Nostoc cyanobacteria in China?

    Science.gov (United States)

    Roney, Britton R; Renhui, Li; Banack, Sandra Anne; Murch, Susan; Honegger, Rosmarie; Cox, Paul Alan

    2009-01-01

    Grown in arid regions of western China the cyanobacterium Nostoc flagelliforme--called fa cai in Mandarin and fat choy in Cantonese--is wild-harvested and used to make soup consumed during New Year's celebrations. High prices, up to $125 USD/kg, led to overharvesting in Inner Mongolia, Ningxia, Gansu, Qinghai, and Xinjiang. Degradation of arid ecosystems, desertification, and conflicts between Nostoc harvesters and Mongol herdsmen concerned the Chinese environmental authorities, leading to a government ban of Nostoc commerce. This ban stimulated increased marketing of a substitute made from starch. We analysed samples purchased throughout China as well as in Chinese markets in the United States and the United Kingdom. Some were counterfeits consisting of dyed starch noodles. A few samples from California contained Nostoc flagelliforme but were adulterated with starch noodles. Other samples, including those from the United Kingdom, consisted of pure Nostoc flagelliforme. A recent survey of markets in Cheng Du showed no real Nostoc flagelliforme to be marketed. Real and artificial fa cai differ in the presence of beta-N-methylamino-L-alanine (BMAA). Given its status as a high-priced luxury food, the government ban on collection and marketing, and the replacement of real fa cai with starch substitutes consumed only on special occasions, it is anticipated that dietary exposure to BMAA from fa cai will be reduced in the future in China.

  10. Design of farm winery façades for the optimisation of indoor natural lighting: a case study

    Directory of Open Access Journals (Sweden)

    Daniele Torreggiani

    2013-06-01

    Full Text Available This paper deals with the theme of daylighting performances of rural buildings, within a broader research context aimed at establishing design criteria for farm wineries. The objective is to benchmark the performances of different window systems in order to define design guidelines directed towards the optimisation of natural lighting to improve visual comfort and reduce energy consumption. A winegrowing and producing farm with standard features in the Emilia- Romagna region, Northern Italy, is considered as a case study. Particular attention was given to the part of the building designated for tasting activities. The study considered several opening solutions in the building envelope, and showed the effectiveness of those involving south façade glazing with appropriate screening systems. Further analyses will aim to investigate the performance of windows distributed on several fronts, including heat balance assessment.

  11. Slicing Method for curved façade and window extraction from point clouds

    Science.gov (United States)

    Iman Zolanvari, S. M.; Laefer, Debra F.

    2016-09-01

    Laser scanning technology is a fast and reliable method to survey structures. However, the automatic conversion of such data into solid models for computation remains a major challenge, especially where non-rectilinear features are present. Since, openings and the overall dimensions of the buildings are the most critical elements in computational models for structural analysis, this article introduces the Slicing Method as a new, computationally-efficient method for extracting overall façade and window boundary points for reconstructing a façade into a geometry compatible for computational modelling. After finding a principal plane, the technique slices a façade into limited portions, with each slice representing a unique, imaginary section passing through a building. This is done along a façade's principal axes to segregate window and door openings from structural portions of the load-bearing masonry walls. The method detects each opening area's boundaries, as well as the overall boundary of the façade, in part, by using a one-dimensional projection to accelerate processing. Slices were optimised as 14.3 slices per vertical metre of building and 25 slices per horizontal metre of building, irrespective of building configuration or complexity. The proposed procedure was validated by its application to three highly decorative, historic brick buildings. Accuracy in excess of 93% was achieved with no manual intervention on highly complex buildings and nearly 100% on simple ones. Furthermore, computational times were less than 3 sec for data sets up to 2.6 million points, while similar existing approaches required more than 16 hr for such datasets.

  12. Valve inlet fluid conditions for pressurizer safety and relief valves for B and W 177-FA and 205-FA plants. Final report. [PWR

    Energy Technology Data Exchange (ETDEWEB)

    Cartin, L.R.; Winks, R.W.; Merchent, J.W.; Brandt, R.T.

    1982-12-01

    The overpressurization transients for the Babcock and Wilcox Company's 177- and 205-FA units are reviewed to determine the range of fluid conditions expected at the inlet of pressurizer safety and relief valves. The final Safety Analysis Report, extended high-pressure injection, and cold overpressurization events are considered. The results of this review, presented in the form of tables and graphs, provide input to the PWR utilities in their justification that the fluid conditions under which their valve designs were tested as part of the EPRI PWR Safety and Relief Valve Test Program are representative of those expected in their unit(s).

  13. Metabolic engineering of E. coli top 10 for production of vanillin through FA catabolic pathway and bioprocess optimization using RSM.

    Science.gov (United States)

    Chakraborty, Debkumar; Gupta, Gaganjot; Kaur, Baljinder

    2016-12-01

    Metabolic engineering and construction of recombinant Escherichia coli strains carrying feruloyl-CoA synthetase and enoyl-CoA hydratase genes for the bioconversion of ferulic acid to vanillin offers an alternative way to produce vanillin. Isolation and designing of fcs and ech genes was carried out using computer assisted protocol and the designed vanillin biosynthetic gene cassette was cloned in pCCIBAC expression vector for introduction in E. coli top 10. Recombinant strain was implemented for the statistical optimization of process parameters influencing F A to vanillin biotransformation. CCD matrix constituted of process variables like FA concentration, time, temperature and biomass with intracellular, extracellular and total vanillin productions as responses. Production was scaled up and 68 mg/L of vanillin was recovered from 10 mg/L of FA using cell extracts from 1 mg biomass within 30 min. Kinetic activity of enzymes were characterized. From LCMS-ESI analysis a metabolic pathway of FA degradation and vanillin production was predicted.

  14. Moringa oleifera Lam.: Protease activity against blood coagulation cascade

    Directory of Open Access Journals (Sweden)

    A Satish

    2012-01-01

    Full Text Available Background : The present study evaluated the protease activity of aqueous extracts of Moringa oleifera (Moringaceae leaf (MOL and root (MOR. Materials and Methods : Protease activity was assayed using casein, human plasma clot and human fibrinogen as substrates. Results : Caseinolytic activity of MOL was significantly higher (P ≤ 0.05 than that of MOR. Similar observations were found in case of human plasma clot hydrolyzing activity, wherein MOL caused significantly higher (P ≤ 0.05 plasma clot hydrolysis than MOR. Zymographic techniques were used to detect proteolytic enzymes following electrophoretic separation in gels. Further, both the extracts exhibited significant procoagulant activity as reflected by a significant decrease (P ≤ 0.05 in recalcification time, accompanied by fibrinogenolytic and fibrinolytic activities; clotting time was decreased from 180 ± 10 sec to 119 ± 8 sec and 143 ± 10 sec by MOL and MOR, respectively, at a concentration of 2.5 mg/mL. Fibrinogenolytic (human fibrinogen and fibrinolytic activity (human plasma clot was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, plate method and colorimetric method. Zymographic profile indicated that both the extracts exerted their procoagulant activity by selectively hydrolyzing Aa and Bb subunits of fibrinogen to form fibrin clot, thereby exhibiting fibrinogenolytic activity. However, prolonged incubation resulted in degradation of the formed fibrin clot, suggesting fibrinolytic like activity. Conclusions : These findings support the traditional usage of M. oleifera extracts for wound healing.

  15. Red wine polyphenols prevent metabolic and cardiovascular alterations associated with obesity in Zucker fatty rats (Fa/Fa.

    Directory of Open Access Journals (Sweden)

    Abdelali Agouni

    Full Text Available BACKGROUND: Obesity is associated with increased risks for development of cardiovascular diseases. Epidemiological studies report an inverse association between dietary flavonoid consumption and mortality from cardiovascular diseases. We studied the potential beneficial effects of dietary supplementation of red wine polyphenol extract, Provinols, on obesity-associated alterations with respect to metabolic disturbances and cardiovascular functions in Zucker fatty (ZF rats. METHODOLOGY/PRINCIPAL FINDINGS: ZF rats or their lean littermates received normal diet or supplemented with Provinols for 8 weeks. Provinols improved glucose metabolism by reducing plasma glucose and fructosamine in ZF rats. Moreover, it reduced circulating triglycerides and total cholesterol as well as LDL-cholesterol in ZF rats. Echocardiography measurements demonstrated that Provinols improved cardiac performance as evidenced by an increase in left ventricular fractional shortening and cardiac output associated with decreased peripheral arterial resistances in ZF rats. Regarding vascular function, Provinols corrected endothelial dysfunction in aortas from ZF rats by improving endothelium-dependent relaxation in response to acetylcholine (Ach. Provinols enhanced NO bioavailability resulting from increased nitric oxide (NO production through enhanced endothelial NO-synthase (eNOS activity and reduced superoxide anion release via decreased expression of NADPH oxidase membrane sub-unit, Nox-1. In small mesenteric arteries, although Provinols did not affect the endothelium-dependent response to Ach; it enhanced the endothelial-derived hyperpolarizing factor component of the response. CONCLUSIONS/SIGNIFICANCE: Use of red wine polyphenols may be a potential mechanism for prevention of cardiovascular and metabolic alterations associated with obesity.

  16. CFD model of air movement in ventilated façade: comparison between natural and forced air flow

    Directory of Open Access Journals (Sweden)

    Miguel Mora Pérez, Gonzalo López Patiño, P. Amparo López Jiménez

    2013-01-01

    Full Text Available This study describes computational fluid dynamics (CFD modeling of ventilated façade. Ventilated façades are normal façade but it has an extra channel between the concrete wall and the (double skin façade. Several studies found in the literature are carried out with CFD simulations about the behavior of the thermodynamic phenomena of the double skin façades systems. These studies conclude that the presence of the air gap in the ventilated façade affects the temperature in the building skin, causing a cooling effect, at least in low-rise buildings. One of the most important factors affecting the thermal effects of ventilated façades is the wind velocity. In this contribution, a CFD analysis applied on two different velocity assumptions for air movement in the air gap of a ventilated façade is presented. A comparison is proposed considering natural wind induced velocity with forced fan induced velocity in the gap. Finally, comparing temperatures in the building skin, the differences between both solutions are described determining that, related to the considered boundary conditions, there is a maximum height in which the thermal effect of the induced flow is significantly observed.

  17. Application of FA-SVM optimization algorithm in emotion recognition%FA-SVM优化算法在情感识别中的应用

    Institute of Scientific and Technical Information of China (English)

    张兢; 曾建梅; 李冠迪; 史文进

    2016-01-01

    SVM有着良好的分类性能,在各种分类实践中得到了广泛应用.针对SVM的关键参数,如惩罚因子C和核函数参数γ选取不当会影响SVM性能的问题,提出采用收敛速度快、寻优精度高的萤火虫算法(FA)对SVM关键参数进行自动寻优,建立FA-SVM分类模型,并将该模型应用于脉搏信号的情感识别中.情感分类结果表明,SVM经FA算法优化后,对样本分类的识别率比未经任何处理的SVM高出7.9%,验证了该方法的有效性.

  18. Mixed compared with single-source proteins in high-protein diets affect kidney structure and function differentially in obese fa/fa Zucker rats.

    Science.gov (United States)

    Devassy, Jessay G; Wojcik, Jennifer L; Ibrahim, Naser H M; Zahradka, Peter; Taylor, Carla G; Aukema, Harold M

    2017-02-01

    Questions remain regarding the potential negative effects of dietary high protein (HP) on kidney health, particularly in the context of obesity in which the risk for renal disease is already increased. To examine whether some of the variability in HP effects on kidney health may be due to source of protein, obese fa/fa Zucker rats were given HP (35% of energy from protein) diets containing either casein, soy protein, or a mixed source of animal and plant proteins for 12 weeks. Control lean and obese rats were given diets containing casein at normal protein (15% of energy from protein) levels. Body weight and blood pressure were measured, and markers of renal structural changes, damage, and function were assessed. Obesity alone resulted in mild renal changes, as evidenced by higher kidney weights, proteinuria, and glomerular volumes. In obese rats, increasing the protein level using the single, but not mixed, protein sources resulted in higher renal fibrosis compared with the lean rats. The mixed-protein HP group also had lower levels of serum monocyte chemoattractant protein-1, even though this diet further increased kidney and glomerular size. Soy and mixed-protein HP diets also resulted in a small number of damaged glomeruli, while soy compared with mixed-protein HP diet delayed the increase in blood pressure over time. Since obesity itself confers added risk of renal disease, an HP diet from mixed-protein sources that enables weight loss but has fewer risks to renal health may be advantageous.

  19. Kodamaea neixiangensis f.a., sp. nov. and Kodamaea jinghongensis f.a., sp. nov., two yeast species isolated from rotting wood.

    Science.gov (United States)

    Gao, Wan-Li; Liu, Tian-Tian; Zheng, Jun; Hui, Feng-Li

    2017-08-25

    Seven strains representing two novel yeast species were isolated from rotting wood in Henan and Yunnan Provinces, PR China. The results of phylogenetic analysis based on the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that these two species are members of the genus Kodamaea, although the formation of ascospores was not observed. Kodamaea neixiangensis f.a., sp. nov. (type strain NYNU 167139T=CICC 33170T=CBS 14699T) formed a clade with Candida kaohsiungensis and Candida hsintzibuensis, from which it differed by 10-16 substitutions in the D1/D2 domain. The ITS sequences of K. neixiangensis sp. nov. differed by 27 substitutions from those of the type strain of C. kaohsiungensis. The most closely related species with a validly published name to Kodamaea jinghongensis f.a., sp. nov. (type strain NYNU 167162T=CICC 33171T=CBS 14700T) was Candida fukazawae, but this differed by 14 substitutions in the D1/D2 domain and by 15 substitutions in the ITS region.

  20. An Experimental Study on Mitigating Alkali Silica Reaction by Using Fly Ash (FA in Combination with Silica Fume and Expanded Perlite Powder (EPP

    Directory of Open Access Journals (Sweden)

    Isneini Mohd

    2016-01-01

    Full Text Available ASR suppression by FA, SF, EPP, FA in combination with SF and EPP were evaluated by both mortar bar and concrete prism test. Mortar bars were made based on JIS A 1146, meanwhile concrete prism bars were casted in accordance with Rilem AAR-3. Both specimens were stored in 40°C 100% R.H. controlled room. Mortar and concrete mixtures used reactive aggregate in pessimum proportion. The results indicated that FA in combination with SF and EPP showed smaller expansion compared to FA. The best of concrete mixtures in reducing expansion is combination of FA with SF (FA15SF10.

  1. Fetal antigen 1 (FA1), a circulating member of the epidermal growth factor (EGF) superfamily

    DEFF Research Database (Denmark)

    Jensen, Charlotte Harken; Krogh, T N; Støving, René Klinkby;

    1997-01-01

    We describe an ELISA technique for quantification of fetal antigen 1 (FA1), a glycoprotein belonging to the EGF-superfamily. The ELISA is based on immunospecifically purified polyclonal antibodies and has a dynamic range of 0.7-5.3 ng/ml, intra- and inter-assay C.V.s of less than 3.2% and an aver......We describe an ELISA technique for quantification of fetal antigen 1 (FA1), a glycoprotein belonging to the EGF-superfamily. The ELISA is based on immunospecifically purified polyclonal antibodies and has a dynamic range of 0.7-5.3 ng/ml, intra- and inter-assay C.V.s of less than 3...

  2. Development, structure and strength properties of PP/PMMA/FA blends

    Indian Academy of Sciences (India)

    Navin Chand; S R Vashishtha

    2000-04-01

    A new type of flyash filled PP/PMMA blend has been developed. Structural and thermal properties of flyash (FA) filled polypropylene (PP)/polymethyl methacrylate (PMMA) blend system have been determined and analysed. Filled polymer blends were developed on a single screw extruder. Strength and thermal properties of FA filled and unfilled PP/PMMA blends were determined. Addition of flyash imparted dimensional and thermal stability, which has been observed in scanning electron micrographs and in TGA plot. Increase of flyash concentration increased the initial degradation temperature of PP/PMMA blend. The increase of thermal stability has been explained based on increased mechanical interlocking of PP/PMMA chains inside the hollow structure of flyash.

  3. Fałszywy znak równości

    Directory of Open Access Journals (Sweden)

    Efraim Zuroff

    2012-12-01

    Full Text Available The equivalency canard Efraim Zuroff's text, originally published in Haaretz magazine, is a review of Timothy Snyder's Bloodlands. Snyder distinguishes six main mass murders commited by Nazi Germany and Soviet Union during the period of the Third Reich's existence. In Zuroff's opinion there are some significant differences between these tragedies. Dubious comparisons proposed by Snyder made the Holocaust most affected. Describing the Shoah as one of the six equally horryfying mass murders, the author ignores its ideological roots. Roots that sentenced to death all Jews  - regardless of their political views, religious practicies or the level of identification with Jewish community. Moreover Snyder takes no notice of the georaphical scope of the Holocaust. He also does not notice the fact, that the Nazis effectively managed to make so many Europeans their accomplices, who actively supported the Shoah.   Fałszywy znak równości Tekst Efraima Zuroffa, który pierwotnie ukazał się w piśmie Haaretz, to recenzja książki Skrwawione ziemie Timothy Snydera. Snyder wyróżnia sześć głównych masowych mordów popełnionych przez Niemcy i Związek Radziecki w okresie, który odpowiada istnieniu Trzeciej Rzeszy. Istnieją jednak, zdaniem Zuroffa, znaczące różnice między tymi tragediami. Na wątpliwych porównaniach, które proponuje Snyder, najbardziej „ucierpiał” Holokaust. Opisując Shoah jako jeden z sześciu równie straszliwych, masowych mordów, autor pomija jego ideologiczne korzenie, które sprawiały, że na śmierć skazany był każdy bez wyjątku Żyd, niezależnie od jego poglądów politycznych, praktyk religijnych czy stopnia identyfikacji z żydowską wspólnotą. Ponadto Snyder ignoruje ogromny zasięg geograficzny Holokaustu. Nie zauważa także skuteczności, z jaką naziści potrafili uczynić swoimi wspólnikami tak wielu Europejczyków, którzy w konsekwencji aktywnie wspomagali Shoah.

  4. Aggressive Recurrence of Oral Squamous Cell Carcinoma in a patient with Fanconi’s Anaemia (FA)

    LENUS (Irish Health Repository)

    Nolan, M.

    2017-03-01

    Fanconi’s Anaemia is a rare autosomal recessive disease for which the incidence of head and neck cancer can be increased 700-fold1. We report a case of a 31-year old Caucasian male with FA who initially presented in July 2007 with oral squamous cell carcinoma for which he received radical surgery and radiotherapy. He was disease-free until August 2015 when he presented with an extremely aggressive recurrence.

  5. FaCSI: A block parallel preconditioner for fluid-structure interaction in hemodynamics

    Science.gov (United States)

    Deparis, Simone; Forti, Davide; Grandperrin, Gwenol; Quarteroni, Alfio

    2016-12-01

    Modeling Fluid-Structure Interaction (FSI) in the vascular system is mandatory to reliably compute mechanical indicators in vessels undergoing large deformations. In order to cope with the computational complexity of the coupled 3D FSI problem after discretizations in space and time, a parallel solution is often mandatory. In this paper we propose a new block parallel preconditioner for the coupled linearized FSI system obtained after space and time discretization. We name it FaCSI to indicate that it exploits the Factorized form of the linearized FSI matrix, the use of static Condensation to formally eliminate the interface degrees of freedom of the fluid equations, and the use of a SIMPLE preconditioner for saddle-point problems. FaCSI is built upon a block Gauss-Seidel factorization of the FSI Jacobian matrix and it uses ad-hoc preconditioners for each physical component of the coupled problem, namely the fluid, the structure and the geometry. In the fluid subproblem, after operating static condensation of the interface fluid variables, we use a SIMPLE preconditioner on the reduced fluid matrix. Moreover, to efficiently deal with a large number of processes, FaCSI exploits efficient single field preconditioners, e.g., based on domain decomposition or the multigrid method. We measure the parallel performances of FaCSI on a benchmark cylindrical geometry and on a problem of physiological interest, namely the blood flow through a patient-specific femoropopliteal bypass. We analyze the dependence of the number of linear solver iterations on the cores count (scalability of the preconditioner) and on the mesh size (optimality).

  6. Multiple Dissipative Devices for Blast-Resisting Cable-Supported Glazing Façades

    Directory of Open Access Journals (Sweden)

    Claudio Amadio

    2013-01-01

    Full Text Available The paper analyzes the structural response of a high-level air blast loaded cable-supported façade. Since the glass panels and the cables present a typical brittle behavior and are subjected to elevated tensile stresses when a high-level explosion occurs, multiple dissipative devices are simultaneously introduced in the conventional glazing system to mitigate the maximum effects of the design blast wave. Dynamic analyses are performed using a sophisticated FE-model to describe accurately the response of the façade equipped by dissipative devices. Based on numerical results of previous contributions, viscoelastic spider connectors (VESCs are introduced in the points of connection between glass panels and pretensioned cables, to replace “rigid” spider connectors commonly used in practice. At the same time, rigid-plastic frictional devices (RPDs are installed at the top of the bearing cables to mitigate furthermore the bracing system. As a result, due to the combined use of VESCs and RPDs opportunely calibrated, the maximum tensile stresses in the glass panels and in the cables appear strongly reduced. In addition, the proposed devices do not trouble the aesthetics of such transparent structural systems. At last, simple design rules are presented to predict the response of cable-supported façades subjected to high-level dynamic loads and to preliminary estimate the mechanical parameters of combined VESCs and RPDs.

  7. EPS insulated façade fires from a fire and rescue perspective

    Directory of Open Access Journals (Sweden)

    Kumm M.

    2013-11-01

    Full Text Available This paper highlights the challenges the fire and rescue services can meet at façade fires involving EPS insulation during construction and use of a building. The EPS characteristics are discussed in respect to the fire and rescue operation and results from orientating fire tests performed at a fire and rescue services training and test field are presented. Types of evacuation solutions, involving the fire and rescue services, where façade fires can delay or completely rule out the possibilities for safe evacuation, are presented. The restrictions in the Swedish building codes regarding use of combustible insulation are analysed and reflections over the practical problems with following the instructions to keep an EPS insulated façade safe through the building's whole lifespan are made. A number of occurred fires involving EPS are discussed and analysed from a fire and rescue perspective. Finally, recommendations are given for the fire and rescue services and future research fields are proposed.

  8. Fire protection concepts for Timber-Glass Composite façades

    Directory of Open Access Journals (Sweden)

    Schleicher Andreas

    2016-01-01

    The main objectives can be summarized as follows: Growth and densification in urban areas require the development of intelligent and resource-efficient building systems for “Smart Cities” of the future. By using timber-glass composites (TGC the primary energy demand of buildings can be reduced substantially. This research project examines the feasibility of applications of this new technology in multi-story and high-rise buildings. Critical aspects concerning fire protection such as flammability of timber elements, fire spread and failure of façade elements with bracing capacity will be analyzed. Different strategies will be developed in case studies and validated by structural analysis. Large scale mock ups of TGC façade elements will be checked on their suitability in fire tests. The findings of this research will lead to innovative fire safety concepts for building systems with TGC façades. Compliance with the high safety standards for multi-story buildings in urban areas like Vienna is one of the main objectives of this work. The adaptation of these fire safety concepts to the national standards of the neighboring countries will be continued subsequently. The gained knowledge should lead to further cooperation with companies for serial productions with TGC technology.

  9. Evaluation of a weight-adjusted single-bolus plasminogen activator in patients with myocardial infarction - A double-blind, randomized angiographic trial of lanoteplase versus alteplase

    NARCIS (Netherlands)

    den Heijer, P; Vermeer, F; Ambrosioni, E; Sadowski, Z; Lopez-Sendon, JL; von Essen, R; Beaufils, P; Thadani, U; Adgey, J; Pierard, L; Brinker, J; Davies, RF; Smalling, RW; Wallentin, L; Caspi, A; Pangerl, A; Trickett, L; Hauck, C; Henry, D; Chew, P

    1998-01-01

    Background-Lanoteplase (nPA) is a rationally designed variant of tissue plasminogen activator with greater fibrinolytic potency and slower plasma clearance than alteplase. Methods and Results-InTIME (Intravenous nPA for Treatment of Infarcting Myocardium Early), a multicenter, double-blind, randomiz

  10. New insights into the operative network of FaEO, an enone oxidoreductase from Fragaria x ananassa Duch.

    Science.gov (United States)

    Collu, Gabriella; Farci, Domenica; Esposito, Francesca; Pintus, Francesca; Kirkpatrick, Joanna; Piano, Dario

    2017-05-01

    The 2-methylene-furan-3-one reductase or Fragaria x ananassa Enone Oxidoreductase (FaEO) catalyses the last reductive step in the biosynthesis of 4-hydroxy-2,5-dimethyl-3(2H)-furanone, a major component in the characteristic flavour of strawberries. In the present work, we describe the association between FaEO and the vacuolar membrane of strawberry fruits. Even if FaEO lacks epitopes for stable or transient membrane-interactions, it contains a calmodulin-binding region, suggesting that in vivo FaEO may be associated with the membrane via a peripheral protein complex with calmodulin. Moreover, we also found that FaEO occurs in dimeric form in vivo and, as frequently observed for calmodulin-regulated proteins, it may be expressed in different isoforms by alternative gene splicing. Further mass spectrometry analysis confirmed that the isolated FaEO consists in the already known isoform and that it is the most characteristic during ripening. Finally, a characterization by absorption spectroscopy showed that FaEO has specific flavoprotein features. The relevance of these findings and their possible physiological implications are discussed.

  11. Differential protection of Cry1Fa toxin against Spodoptera frugiperda larval gut proteases by cadherin orthologs correlates with increased synergism.

    Science.gov (United States)

    Rahman, Khalidur; Abdullah, Mohd Amir F; Ambati, Suresh; Taylor, Milton D; Adang, Michael J

    2012-01-01

    The Cry proteins produced by Bacillus thuringiensis (Bt) are the most widely used biopesticides effective against a range of crop pests and disease vectors. Like chemical pesticides, development of resistance is the primary threat to the long-term efficacy of Bt toxins. Recently discovered cadherin-based Bt Cry synergists showed the potential to augment resistance management by improving efficacy of Cry toxins. However, the mode of action of Bt Cry synergists is thus far unclear. Here we elucidate the mechanism of cadherin-based Cry toxin synergism utilizing two cadherin peptides, Spodoptera frugiperda Cad (SfCad) and Manduca sexta Cad (MsCad), which differentially enhance Cry1Fa toxicity to Spodoptera frugiperda neonates. We show that differential SfCad- and MsCad-mediated protection of Cry1Fa toxin in the Spodoptera frugiperda midgut correlates with differential Cry1Fa toxicity enhancement. Both peptides exhibited high affinity for Cry1Fa toxin and an increased rate of Cry1Fa-induced pore formation in S. frugiperda. However, only SfCad bound the S. frugiperda brush border membrane vesicle and more effectively prolonged the stability of Cry1Fa toxin in the gut, explaining higher Cry1Fa enhancement by this peptide. This study shows that cadherin fragments may enhance B. thuringiensis toxicity by at least two different mechanisms or a combination thereof: (i) protection of Cry toxin from protease degradation in the insect midgut and (ii) enhancement of pore-forming ability of Cry toxin.

  12. Spread of smoke and heat along narrow air cavity in double-skin façade fires

    Directory of Open Access Journals (Sweden)

    Chow Lun Cheuk

    2014-01-01

    Full Text Available A scenario on double-skin façade fire was identified earlier for hazard assessment. A flashover room fire occurred next to the façade, broke the interior glass pane and spread to the façade cavity. As observed in experiments, hot gas moved up as a vertical channel flow for narrow façade cavity. Heat and smoke spread along the narrow air cavity of a double-skin façade will be studied in this paper. A simple mathematical model is developed from basic heat transfer theory for studying the vertical air temperature profiles of the hot gas flowing along the cavity. Assuming one-dimensional flow for hot gas moving up the façade cavity, conservation equations on mass and enthalpy were solved. Experimental results on two double-skin façade rigs of height 6 m and 15 m with narrow cavity depth were used to justify the results. A total of 11 tests were carried out. Correlation expressions between cavity air temperature and the height above ceiling of the fire room were derived.

  13. Nutritional Stress Causes Heterogeneous Relationships with Multi-Trait FA in Lesser Black-Backed Gull Chicks: An Aviary Experiment

    Directory of Open Access Journals (Sweden)

    Trisha Gupta

    2016-11-01

    Full Text Available Environmental stressors have the potential to induce perturbations in the development of young individuals, leading to aberrant and unstable development. This may manifest as fluctuating asymmetry (FA; small, non-directional changes in the bilateral symmetry of morphological traits. Although widely regarded as a proxy for stress effects, the use of FA as a biomarker is still a topic of much debate. We investigated the applicability of FA as an indicator of nutritional stress (brought about by energetic constraints by experimental manipulation of the diet composition and quantity during the growth of Lesser Black-backed Gull (Larus fuscus chicks. FA as an endpoint was measured across the tarsus, wing and 10th primary feather when chicks reached 30 days of age. Although levels of asymmetry were found to increase with stress in the feather, relationships with tarsus and wing FA were mixed and mostly non-significant. Furthermore, we did not find any correlations in unsigned FA between traits, indicating the absence of organism-wide asymmetry. Our study was therefore unable to find unequivocal evidence in support of the application of FA as a reliable estimator of nutritional stress.

  14. "Near-bottom sonar mapping along the Eastern Lau Spreading Center and Valu Fa Ridge"

    Science.gov (United States)

    Sleeper, J. D.; Martinez, F.; Edwards, M.; Dunn, R.; Baker, E. T.

    2009-12-01

    We present results from high resolution sidescan sonar mapping at the Ridge 2000 Lau Integrated Studies Site, a back-arc spreading center in the Southwest Pacific. The survey covered segments of the Eastern Lau Spreading Center (ELSC) and Valu Fa Ridge (VFR), which exhibit strong contrasts in geologic and geophysical characteristics, including variations in ridge morphology, volcanic and tectonic structures, lava composition and texture, magma production, and spreading rate. The bulk of the data were collected in 2008 aboard R/V Kilo Moana (KM0804), using the deep-towed IMI120 (DSL120A) sonar, with an array of miniature autonomous plume recorder's (MAPR's) tethered to the tow cable to detect hydrothermal plume signatures in the water column. The ELSC survey covered an area ~10 km wide by 100 km long centered on the spreading axis, with tracks spaced ~1 km apart, but as close as ~500 m in the vicinity of the ABE hydrothermal vent field, and also included the Tow Cam and Kilo Moana vent fields. The VFR survey covered a ~5 km wide by 100 km long area with ~700 m-spaced tracks, including the Vai Lili, Mariner, and Tui Malila vent sites. The IMI120 has a resolution of ~1-2 meters, giving the first detailed view of the on- and off-axis structures in the two focus regions. Navigational corrections to the sidescan data were made through feature-matching between the deep-towed data and a lower resolution background ship bathymetry grid, using software developed by the Hawaiian Mapping and Research Group. Background bathymetry and lower resolution (~100 m) sidescan data were compiled from previous cruises on R/V Kilo Moana, and an early-2009 seismic survey on R/V Marcus G. Langseth (L-SCAN). Hydrothermal plume data collected with the MAPR's are coregistered with the deep-towed sidescan data to illuminate correlations between seafloor structures and plume signatures. The axis of the VFR in the south forms a peaked ridge, inflated by abundant melt supply, and draped with

  15. Mouse fetal antigen 1 (mFA1), the circulating gene product of mdlk, pref-1 and SCP-1: isolation, characterization and biology

    DEFF Research Database (Denmark)

    Bachmann, E; Krogh, T N; Højrup, P

    1996-01-01

    The mouse homologue to human fetal antigen 1 (hFA1) was purified from mouse amniotic fluid by cation exchange chromatography and immunospecific affinity chromatography. Mouse FA1 (mFA1) is a single chain glycoprotein with an M(r) of 42-50 kDa (SDS-PAGE). The N-terminal amino acid sequence (39 res...

  16. Process Improvement at the F/A-18 Organizational Maintenance Activity

    Science.gov (United States)

    2008-01-01

    martial arts levels and are targeted toward employees, project leaders, and quality leaders. The highest level is targeted toward quality leaders and is... martial arts , etc and there is very little if any room for unnecessary training. However, the benefits of training specific billets and crews will far

  17. AFSC/ABL: Origins of sockeye and chum salmon seized from the F/V Ying Fa

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Samples of chum (Oncorhynchus keta) and sockeye (O. nerka) salmon seized from the stateless fishing vessel Ying Fa were analyzed to determine their region of origin...

  18. Cercetări experimentale privind comportarea sistemelor pentru faţade, expuse la acţiunea focului

    Directory of Open Access Journals (Sweden)

    Ştefania Rădulescu

    2010-12-01

    Full Text Available Visually, it is often the goal of any architecture to define a personality or individual character through the design of the façade. This face or skin, wrapped to the structural frame beneath, is often key to an architect's desire to evoke our emotions, instilling a sense of grandeur as if each new building were an artist's sculpture. In recent decades the desire for taller structures and, particularly, those that are competing for recognition to be among the tallest, if not the world's tallest, is reason to review the fire safety issues related to façade or curtain wall design. Additionally, due to the creativity of architects, new and unique façade designs are continually appearing. The risk of fire spread through articulated elements of the façade or vertically around the façade via the mechanism of flame leap, poses new concerns for the newest class of super highrise structures. Our understanding of fire and its mechanisms of spread in buildings no longer eludes us, however, the risks of fire spread related to super high-rise buildings and the façades that define their character has not been well examined. Current code practices recognize the successful record of fully sprinkler protected high-rise buildings and only require that the void space between the curtain wall and the floor slab be resistive to fire spread using a perimeter fire barrier system.

  19. The therapeutic potential of Rho kinase inhibitor fasudil derivative FaD-1 in experimental autoimmune encephalomyelitis.

    Science.gov (United States)

    Zhao, Yong-Fei; Zhang, Xiang; Ding, Zhi-Bin; Yang, Xing-Wang; Zhang, Hui; Yu, Jie-Zhong; Li, Yan-Hua; Liu, Chun-Yun; Zhang, Qing; Zhang, Hong-Zhen; Ma, Cun-Gen; Xiao, Bao-Guo

    2015-03-01

    Although therapeutic potential of fasudil in EAE is promising, action mechanism and clinical limitations are still not fully understood and resolved. In this study, we observed the therapeutic potential of a novel Rho kinase (ROCK) inhibitor FaD-1, a fasudil derivative, and explored possible mechanism in MOG35-55-induced EAE. Experimental autoimmune encephalomyelitis (EAE) was induced by myelin oligodendrocyte glycoprotein (MOG35-55) immunization. The pathology of spinal cord was measured by immunohistochemistry and neurological impairment was evaluated using clinical scores. FaD-1, as a novel ROCK inhibitor, inhibited the expression of ROCK II that is mainly expressed in the CNS. We show here that FaD-1 ameliorates the neurological defects and the severity of MOG-induced EAE in mice, accompanied by the protection of demyelination and the inhibition of neuroinflammation in spinal cord of EAE. In addition, FaD-1 dampened TLR2 and TLR4 signaling as well as Th1 (IFN-γ) and Th17 (IL-17) responses in spinal cord of EAE. FaD-1 also prevented the expression of iNOS and production of inflammatory cytokine IL-1β, IL-6, and TNF-α which are specific markers for M1 inflammatory microglia/macrophages. This study highlights the therapeutic potential of FaD-1 as a ROCK inhibitor for the treatment of human autoimmune diseases with both inflammatory and autoimmune components.

  20. Birth order and recalled childhood gender nonconformity in Samoan men and fa'afafine.

    Science.gov (United States)

    Semenyna, Scott W; VanderLaan, Doug P; Vasey, Paul L

    2017-04-01

    Having a greater than average number of older biological brothers is a robust correlate of male androphilia (i.e., sexual attraction and arousal to adult males). Previous investigations have sought to understand whether this fraternal birth order (FBO) effect is also systematically related to recalled indicators of childhood gender nonconformity (CGN). However, these investigations have relied on data from low-fertility Western populations in which expressions of femininity in male children are routinely stigmatized and consequently, suppressed. The present study examined the FBO effect (among other sibship characteristics) and recalled indicators of CGN in Samoa, a high-fertility population, whose members are relatively tolerant of male femininity. Indeed, Samoans identify feminine androphilic males as belonging to an alternative gender category, known locally as fa'afafine. The present study compared the sibship characteristics of 231 fa'afafine and 231 opposite-sex attracted men from Samoa, as well as how these characteristics related to recalled CGN. Results replicated the well-established FBO effect for predicting male sexual orientation, with each older brother increasing the odds of being androphilic by 21%. However, no relationship was found between the number of older brothers (or other siblings) a participant had and their recalled CGN. Although fa'afafine reported significantly more CGN than Samoan men, CGN did not mediate the FBO effect, nor did the FBO effect and CGN interact to predict male sexual orientation. These findings are consistent with previous studies suggesting that the FBO effect is associated with male sexual orientation, but not childhood female-typical gender expression among androphilic males.

  1. Development of a Façade Assessment and Design Tool for Solar Energy (FASSADES)

    OpenAIRE

    Jouri Kanters; Maria Wall; Marie-Claude Dubois

    2014-01-01

    Planning energy-efficient buildings which produce on-site renewable energy in an urban context is a challenge for all involved actors in the planning process. The primary objective of this study was to develop a façade assessment and design tool for solar energy (FASSADES) providing the necessary information for all stakeholders in the design process. The secondary objective was to demonstrate the tool by performing an assessment analysis of a building block. The FASSADES tool is a DIVA4Rhino...

  2. Increasing Ranks of Linear Finite Automata and Complexity of FA Public Key Cryptosystem

    Institute of Scientific and Technical Information of China (English)

    鲍丰

    1994-01-01

    The encryption algorithm of finite automata (FA) public key cryptosystem is implemented by a weakly invertible finite automata (WIFA) which is composed of a nonlinear WIFA with delay 0 and a linear WIFA with delay τ. In this paper, we proved that such an automaton bears the same properties as the linear WIFA and the increasing ranks of the latter are key factors to affecting the former. A probabilistic algorithm is given to realize a ciphertext attack, and its complexity is analysed through the increasing ranks of the linear WIFA. The size of the parameters for safe linear WIFA is estimated.

  3. Objective and subjective evaluation of façade sound insulation

    DEFF Research Database (Denmark)

    Ordoñez, Rodrigo Pizarro; Visentin, Chiara; Markovic, Milos

    2013-01-01

    Façade insulation of several different construction types were subjectively evaluated using two psychoacoustic methods: paired comparisons using a two alternative forced choice (2-AFC) paradigm and direct scaling using a visual analogue scale (VAS). The stimuli used in the evaluations were obtained...... by filtering recordings of traffic noise with the frequency response of sound insulation measurements. The measurements were performed in typical Italian buildings in accordance with the ISO 140-5 standard. The objectives of the present paper are to compare the subjective evaluations obtained with the two...... psychoacoustic methods, and to investigate the correlation between subjective assessments and objective ratings in different construction types....

  4. Una experiencia en GPU Computing entre FaMAF e INVAP.

    OpenAIRE

    Bustos, Fabio; Wolovick, Nicolás

    2014-01-01

    Mostramos la experiencia realizada entre INVAP SE y FaMAF-UNC, para el desarrollo del software de imágenes sobre placas de procesamiento gráfico de propósitos generales (GPGPU por sus siglas en inglés). En el contexto de un sistema de adquisición de imágenes desarrollado por INVAP para uno de sus clientes, resulta necesario incluir un módulo de software capaz de realizar un seguimiento automático de puntos de interés identificados en un video....

  5. Isolation and Characterization of Frankia sp. Strain FaC1 Genes Involved in Nitrogen Fixation

    OpenAIRE

    Ligon, James M.; James P. Nakas

    1987-01-01

    Genomic DNA was isolated from Frankia sp. strain FaC1, an Alnus root nodule endophyte, and used to construct a genomic library in the cosmid vector pHC79. The genomic library was screened by in situ colony hybridization to identify clones of Frankia nitrogenase (nif) genes based on DNA sequence homology to structural nitrogenase genes from Klebsiella pneumoniae. Several Frankia nif clones were isolated, and hybridization with individual structural nitrogenase gene fragments (nifH, nifD, and n...

  6. Non-receptor-mediated actions are responsible for the lipid-lowering effects of iodothyronines in FaO rat hepatoma cells.

    Science.gov (United States)

    Grasselli, Elena; Voci, Adriana; Canesi, Laura; Goglia, Fernando; Ravera, Silvia; Panfoli, Isabella; Gallo, Gabriella; Vergani, Laura

    2011-07-01

    Iodothyronines influence lipid metabolism and energy homeostasis. Previous studies demonstrated that 3,5-l-diiodothyronine (T(2)), as well as 3,3',5-L-triiodothyronine (T(3)), was able to both prevent and reverse hepatic steatosis in rats fed a high-fat diet, and this effect depends on a direct action of iodothyronines on the hepatocyte. However, the involvement of thyroid hormone receptors (TRs) in mediating the lipid-lowering effect of iodothyronines was not elucidated. In this study, we investigated the ability of T(2) and T(3) to reduce the lipid overloading using the rat hepatoma FaO cells defective for functional TRs. The absence of constitutive mRNA expression of both TRα1 and TRβ1 in FaO cells was verified by RT-qPCR. To mimic the fatty liver condition, FaO cells were treated with a fatty acid mixture and then exposed to pharmacological doses of T(2) or T(3) for 24 h. Lipid accumulation, mRNA expression of the peroxisome proliferator-activated receptors (PPAR-α, -γ, -δ) the acyl-CoA oxidase (AOX), and the stearoyl CoA desaturase (SCD1), as well as fuel-stimulated O(2) consumption in intact cells, were evaluated. Lipid accumulation was associated with an increase in triacylglycerol content, PPARγ mRNA expression, and a decrease in PPARδ and SCD1 mRNA expression. The addition of T(2) or T(3) to lipid-overloaded cells resulted in i) reduction in lipid content; ii) downregulation of PPARα, PPARγ, and AOX expression; iii) increase in PPARδ expression; and iv) stimulation of mitochondrial uncoupling. These data demonstrate, for the first time, that in the hepatocyte, the lipid-lowering actions of both T(2) and T(3) are not mediated by TRs.

  7. Delta-like 1/fetal antigen-1 (Dlk1/FA1) is a novel regulator of chondrogenic cell differentiation via inhibition of the Akt kinase-dependent pathway

    DEFF Research Database (Denmark)

    Chen, Li; Qanie, Diyako; Jafari, Abbas

    2011-01-01

    Delta-like 1 (Dlk1, also known as fetal antigen-1, FA1) is a member of Notch/Delta family that inhibits adipocyte and osteoblast differentiation; however, its role in chondrogenesis is still not clear. Thus, we overexpressed Dlk1/FA1 in mouse embryonic ATDC5 cells and tested its effects...... on chondrogenic differentiation. Dlk1/FA1 inhibited insulin-induced chondrogenic differentiation as evidenced by reduction of cartilage nodule formation and gene expression of aggrecan, collagen Type II and X. Similar effects were obtained either by using Dlk1/FA1-conditioned medium or by addition of a purified......, secreted, form of Dlk1 (FA1) directly to the induction medium. The inhibitory effects of Dlk1/FA1 were dose-dependent and occurred irrespective of the chondrogenic differentiation stage: proliferation, differentiation, maturation, or hypertrophic conversion. Overexpression or addition of the Dlk1/FA1...

  8. Thermal Performance of Ventilated Double Skin Façades with Venetian Blinds

    Directory of Open Access Journals (Sweden)

    Jordi Parra

    2015-05-01

    Full Text Available Venetian blinds (VB are shading devices of widespread use in residential and corporate buildings. They can reflect or transmit light into buildings and at the same time allow daylighting and exterior views. They can also efficiently block radiative heat from entering the building, and if combined with a heat dissipation system such as forced ventilation, they can improve the thermal performance of double skin façades (DSF. Computational Fluid Dynamics (CFD has proven to be a useful tool for modeling flow and heat transfer in DSF, including conduction, convection and radiation heat transfer phenomena. The aim of this work is to evaluate, by means of CFD, the influence of several optical, construction and operation parameters of a DSF (such as optical properties of the materials, geometrical relations of the VB or flow stream conditions in terms of energy savings, measured as a reduction of the solar load entering the building. Results obtained show that parameters such as the proximity of the VB to the exterior skin of the façade or a differentiated surface treatment for the exterior and interior faces of the VB louvers can notably affect the thermal performance of the DSF and hence the heat gains experienced by the building.

  9. Ultrathin Fluidic Laminates for Large-Area Façade Integration and Smart Windows.

    Science.gov (United States)

    Heiz, Benjamin P V; Pan, Zhiwen; Lautenschläger, Gerhard; Sirtl, Christin; Kraus, Matthias; Wondraczek, Lothar

    2017-03-01

    Buildings represent more than 40% of Europe's energy demands and about one third of its CO2 emissions. Energy efficient buildings and, in particular, building skins have therefore been among the key priorities of international research agendas. Here, glass-glass fluidic devices are presented for large-area integration with adaptive façades and smart windows. These devices enable harnessing and dedicated control of various liquids for added functionality in the building envelope. Combining a microstructured glass pane, a thin cover sheet with tailored mechanical performance, and a liquid for heat storage and transport, a flat-panel laminate is generated with thickness adapted to a single glass sheet in conventional windows. Such multimaterial devices can be integrated with state-of-the-art window glazings or façades to harvest and distribute thermal as well as solar energy by wrapping buildings into a fluidic layer. High visual transparency is achieved through adjusting the optical properties of the employed liquid. Also secondary functionality, such as chromatic windows, polychromatism, or adaptive energy uptake can be generated on part of the liquid.

  10. The Arabic Hyperbolic Pattern 'Fa??al' in Two Recent Translations of the Qur'an

    Directory of Open Access Journals (Sweden)

    Amr M. El-Zawawy

    2014-06-01

    Full Text Available The present study addresses the problem of rendering the فعال'fa??al' hyperbolic pattern into English in two recent translations of the Qur'an. Due to the variety of Qur'an translations and the large amount of hyperbolic forms of Arabic verbs recorded in the Qur'an, only two translations of the Qur'an are consulted and analyzed: these two translations, namely Saheeh International Translation (1997 and Prof. Abdel-Haleem's (2004, are distinguished by the fact that they are recent and well-received. Moreover, the investigation of hyperbolic forms is confined to the Arabic formفعال    'fa??al'. The study reveals that the Saheeh translator has applied morphological shifting in many examples while Abdel-Haleem's translation exhibits a considerable amount of syntactic transposition, coupled with paraphrasing. The test of accuracy as administered here is to give a clear picture of the need to pay particular attention to hyperboles of the form examined and other ones not analyzed here for limitations of space.

  11. Color Image Evaluation for Small Space Based on FA and GEP

    Directory of Open Access Journals (Sweden)

    Li Deng

    2014-01-01

    Full Text Available Aiming at the problem that color image is difficult to quantify, this paper proposes an evaluation method of color image for small space based on factor analysis (FA and gene expression programming (GEP and constructs a correlation model between color image factors and comprehensive color image. The basic color samples of small space and color images are evaluated by semantic differential method (SD method, color image factors are selected via dimension reduction in FA, factor score function is established, and by combining the entropy weight method to determine each factor weights then the comprehensive color image score is calculated finally. The best fitting function between color image factors and comprehensive color image is obtained by GEP algorithm, which can predict the users’ color image values. A color image evaluation system for small space is developed based on this model. The color evaluation of a control room on AC frequency conversion rig is taken as an example, verifying the effectiveness of the proposed method. It also can assist the designers in other color designs and provide a fast evaluation tool for testing users’ color image.

  12. Comparison and development of sustainable office façade renovation solutions in the Netherlands

    Directory of Open Access Journals (Sweden)

    Michiel Ritzen

    2013-12-01

    Full Text Available Environmental, commercial and societal developments in the Netherlands stimulate the environmental improvement of the existing office building stock. In the Netherlands, about 15% of all office area was vacant in 2012, and the majority of offices have a relative poor energy performance. To measure the improvement, different assessment tools are applied. These tools either focus on one aspect, such as operation energy, and result in a specific outcome such as MJ/m2, or these tools combine different aspects, such as energy and materials, through a weighted system and result in a generic outcome, such as ‘excellent’.In this research, the relation between assessment outcome and actual environmental impact is investigated of both types of tools, by reflecting the outcome of the tool to the carrying capacity of a system. The relation is investigated through a comparison of the energy and material aspect of three office façade renovation solutions using four different assessment tools. Using a tool in which energy and material impact is related to the carrying capacity, current energy focused optimization might lead to a sub optimization of actual environmental impact. To illustrate this, a calculated façade solution is presented with minimal environmental impact based on carrying capacity.

  13. Function and principle innovative design of mechanical products based on TRIZ/FA

    Institute of Scientific and Technical Information of China (English)

    YUAN Feng; WANG Tai-yong; NIE Hui-juan

    2006-01-01

    Function and principle innovation is the ultimate innovation of a product.To achieve Function and principle innovation design of mechanical products,a new process model of function and principle solving is proposed,based on TRIZ and FA.In this model,abstraction of general function,confirmation of principle solution to general function,and functional decomposition were included in FA.Confirmation of principle solution to general function was the premise of functional decomposition.To provide a dependable pattern for functional decomposition,a new functional analysis method was put forward based on the generalized process function along with the introduction of the input constraint function;and its five-decomposition-steps were also presented.To provide powerful support for innovation design tools in principle solution,some TRIZ tools in the model were imbedded,such as SU-field Analysis,Scientific Effects,and Contradiction Solving Principles so as to boost up the innovation of principle solution.As a case study,an innovative design of paint-bottling machine has been achieved with application of the proposed model.

  14. Design of MiSolFA Hard X-Ray Imager

    Science.gov (United States)

    Lastufka, Erica; Casadei, Diego

    2017-08-01

    Advances in the study of coronal electron-accelerating regions have so far been limited by the dynamic range of X-ray instruments. A quick and economical alternative to desirable focusing optics technology is stereo observation. The micro-satellite MiSolFA (Micro Solar-Flare Apparatus) is designed both as a stand-alone X-ray imaging spectrometer and a complement to the Spectrometer/Telescope for Imaging X-rays (STIX) mission. These instruments will be the first pair of cross-calibrated X-ray imaging spectrometers to look at solar flares from very different points of view. MiSolFA will achieve indirect imaging between 10 and 60 keV and provide spectroscopy up to 100 keV, equipped with grids producing moiré patterns in a similar way to STIX. New manufacturing techniques produce gold gratings on a graphite or silicon substrate, with periods ranging from 15 to 225 micrometers, separated by a distance of 15.47 cm, to achieve a spatial resolutions from 10" to 60" (as compared to RHESSI's separation of 150 cm and 1" resolution). We present the progress of the imager design, the performance of the first prototypes, and reach out to the community for further scientific objectives to consider in optimizing the final design.

  15. The Development of Thung Fa Bot Weekend Market into Cultural Tourism Attraction in San Pa Tong District, Chiang Mai Province

    Directory of Open Access Journals (Sweden)

    Phuwanatwichit Thongchai

    2014-01-01

    Full Text Available This study aimed to investigate for the socio-cultural and economic context, along with the issues of tourism potentiality of Thung Fa Bot weekend market into the cultural tourism attraction in San Pa Tong District, Chiang Mai Province. A study was created containing Mixed Methodology Research. The data collection was focus group and in-depth interviews from the target population and questionnaires of stakeholders. The study was determined that context of socio-cultural and economic structure,which was relying on farming in the past. The potentiality level to be the cultural tourist attraction in overall was moderate.the aspect of tourist Attraction and Spirit of a community were in high level. On the other hand Accessibility, Activities and Participation civil organizations were in moderate level. There were only the Amenities and Ancillary Services had shown on low level. The results suggested the need to develop its potentiality of cultural tourist attraction in [1] Amenities and Ancillary Services management at the tourist spots and tourism promoting and [2] Linking network with government agencies, private sectors, and communities to join in tourism management in order to enlarge Thung Fha Bod Weekend Market becoming the greater cultural tourism attraction for the tourists.

  16. A xylanase from Streptomyces sp. FA1: heterologous expression, characterization, and its application in Chinese steamed bread.

    Science.gov (United States)

    Xu, Yang; Wu, Jing; Zheng, Kaixuan; Wu, Dan

    2016-05-01

    Xylanases (EC 3.2.1.8) are hydrolytic enzymes that have found widespread application in the food, feed, and paper-pulp industries. Streptomyces sp. FA1 xynA was expressed as a secreted protein in Pichia pastoris, and the xylanase was applied to the production of Chinese steamed bread for the first time. The optimal pH and the optimal temperature of XynA were 5.5 and 60 °C, respectively. Using beechwood as substrate, the K m and V max were 2.408 mg mL(-1) and 299.3 µmol min(-1) mg(-1), respectively. Under optimal conditions, a 3.6-L bioreactor produced 1374 U mL(-1) of XynA activity at a protein concentration of 6.3 g L(-1) after 132 h of fermentation. Use of recombinant XynA led to a greater increase in the specific volume of the CSB than could be achieved using commercial xylanase under optimal conditions. This study provides the basis for the application of the enzyme in the baking industry.

  17. IL1B and VWF variants are associated with fibrinolytic early recanalization in patients with ischemic stroke.

    Science.gov (United States)

    Fernández-Cadenas, Israel; Del Río-Espínola, Alberto; Giralt, Dolors; Domingues-Montanari, Sophie; Quiroga, Adoracion; Mendióroz, Maite; Ruíz, Agustin; Ribó, Marc; Serena, Joaquin; Obach, Victor; Freijo, Mari Mar; Martí-Fábregas, Joan; Delgado, Pilar; Montaner, Joan

    2012-10-01

    There is a great interindividual variability among patients with acute ischemic stroke regarding the response to intravenous tissue-type plasminogen activator treatment. The aim of this study was to identify genetic variants associated with recanalization, and thus treatment efficacy, after tissue-type plasminogen activator administration. A total of 140 single nucleotide polymorphisms from 97 candidate genes were successfully genotyped by SNPlex in 2 cohorts, accounting for 497 prospectively recruited tissue-type plasminogen activator-treated patients, of whom 33% recanalized during tissue-type plasminogen activator infusion. Functional studies were then performed, including assessment of interleukin 1B mRNA levels and von Willebrand factor, FIII, FVII, FVIII, and FX protein activity. After replication, the following single nucleotide polymorphisms were associated with early recanalization: rs1143627 in IL1B gene (CC: 53.1% of recanalization, A-carriers: 32.7%; P=0.022; replication cohort: P=0.046), rs16944 in IL1B gene (AA: 50% of recanalization, G-carriers: 32%; P=0.038; replication cohort: P=0.049), and rs1063856 in the vWF gene (GG: 53.8% of recanalization, A-carriers: 31.5%; P=0.006; replication cohort: P=0.046). The functional studies revealed an association between the rs1063856 single nucleotide polymorphisms in vWF and FVIII activity (AA: 115.93%, AG: 156.07%, GG: 83.42%; P=0.005). Three single nucleotide polymorphisms were associated with tissue-type plasminogen activator efficacy in the Spanish population, and their mechanism of action might be associated with the activity of coagulation factors.

  18. Cloning and Expression Analysis of Cysteine Protease Gene (FaCP) in Strawberry (Fragaria × ananassa)%草莓半胱氨酸蛋白酶基因(FaCP)的克隆及表达分析

    Institute of Scientific and Technical Information of China (English)

    朱海生; 陈敏氡; 温庆放; 林珲

    2013-01-01

    Cysteine protease (CP) is one of the important hydrolysis protease, which widely participates in a variety of physiological processes of plants. The FaCP gene cDNA was cloned from strawberry (Fragaria x ananassa) using RT-PCR and RACE techniques. The cDNA sequence was 1 338 bp (GenBank accession number: JN979371), including 1 065 bp of open reading frame (ORF), which encoded a protein of 354 amino acids with a predicted molecular weight of 39 130 D and a hypothetical pI (isoelectric point) of 4.93. Homology analysis showed that the deduced CP protein was highly homologous to other CP proteins from different plant species. Phylogenetic analysis indicated that FaCP was more related to castor, trichocarpa, kiwi and grape. Real-time PCR analysis revealed FaCP could be expressed in different strawberry tissues including root, stem, leaf, calyx and fruit, of which the highest expression level in fruit. The FaCP expressed continuously during the whole period of strawberry fruit development and reached the maximum at the pink ripening stage and decreased slightly at the red ripening stage. The expression level of FaCP gene in leaves was gradually increased with leaf senescence, which the expression in the young leaves stage (leaf unfolding within 15 days) was low, but in the old leaves (leaf unfolding for more than 45 days), the FaCP had a higher expression. This result showed that FaCP may play a role in the fruit ripening and leaf senescence.%半胱氨酸蛋白酶作为一种重要的水解蛋白酶,参与植物的许多生理过程.采用RT-PCR和RACE技术从草莓(Fragaria×ananassa)中克隆到半胱氨酸蛋白酶基因FaCP (GenBank登录号:JN979371),该基因cDNA全长1 338 bp,包含一个1 062 bp完整的开放阅读框(ORF),编码354氨基酸.生物信息学序列分析表明,FaCP开放阅读框编码的氨基酸序列与其他植物的CP蛋白同源性较高.Real-time PCR分析发现,FaCP基因在草莓果实、叶、根、茎和花萼中都有表达;在果

  19. Color Vision and the Railways: Part 3. Comparison of FaLant, OPTEC 900, and Railway LED Lantern Tests.

    Science.gov (United States)

    Dain, Stephen J; Casolin, Armand; Long, Jennifer

    2015-02-01

    The Farnsworth Lantern (FaLant) and the OPTEC 900 are nominated in the Commission Internationale de l'Éclairage (CIE) Color Vision Standard 2. Neither test uses the railway signal color code of red, yellow, and green, and only the OPTEC 900 is commercially available. The Railway LED Lantern Test (RLLT) is based on railway signaling practices in New South Wales, Australia, and is nominated in the Australian railway medical standard. The objective of this study is to compare the performance of the three lantern tests. The RLLT, FaLant, and OPTEC 900 were administered to 46 color vision-normal and 37 color vision-deficient (CVD) subjects. The pattern of errors on the RLLT was different from that of the FaLant and OPTEC 900. This may be accounted for, at least in part, by the different colors and the use of blank presentations in the RLLT. The three lanterns showed agreement in failing 21 and passing 6 of the CVD subjects (72.9%). The lanterns gave different results for 10 CVD subjects (27.9%): n = 5 passed only the RLLT and n = 3 passed only the FaLant; n = 1 failed only the FaLant and n = 1 failed only the RLLT. The overall failure rate by CVD for each lantern was 67.6% (RLLT), 73.0% (FaLant), and 78.4% (OPTEC 900). Despite the different construction principles, the pass/fail levels of the RLLT, FaLant, and OPTEC 900 are comparable and consistent with the performance of other lanterns listed by the CIE for Color Vision Standard 2. The RLLT may be a little easier to pass and is based on the signal color code used and actual signaling practice. We propose that the RLLT is also an appropriate lantern for CIE Color Vision Standard 2.

  20. Computer-simulation study on fire behaviour in the ventilated cavity of ventilated façade systems

    Directory of Open Access Journals (Sweden)

    Giraldo María P.

    2013-11-01

    Full Text Available Fire spread through the façades is widely recognized as one of the fastest pathways of fire spreading in the buildings. Fire may spread through the façade in different ways depending on the type of façade system and on the elements and materials from which it is constructed. Ventilated façades are multilayer systems whose main feature is the creation of an air chamber of circulating air between the original building wall and the external cladding. The “chimney effect” in the air cavity is a mechanism that improves the façade's thermal behaviour and avoids the appearance of moisture from rain or condensation. However, in a event of fire, it may contribute to the quickest spreading of fire, representing a significant risk to the upper floors of a building. This study deals with some aspects of fire propagation through the ventilated cavity in ventilated façade systems. Also we review the provisions stipulated by the Spanish building code (Código Técnico de la Edificación, CTE [1] to avoid fire spread outside the building. The results highlight the importance of the use of proper fire barriers to ensure the compartmentalization of the ventilated cavity, as well as the use of non-combustible thermal insulation materials, among others. In addition, based on the results, it might be considered that the measures stipulated by the CTE are insufficient to limit the risks associated with this kind of façades systems. The study has been performed using field models of computational fluid-dynamics. In particular, the Fire Dynamics Simulator (FDS software has been used to numerically solve the mathematical integration models.

  1. Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential.

    Science.gov (United States)

    Boncela, Joanna; Smolarczyk, Katarzyna; Wyroba, Elzbieta; Cierniewski, Czeslaw S

    2006-01-13

    Our previous studies showed that thymosin beta4 (Tbeta4) induced the synthesis of plasminogen activator inhibitor-1 (PAI-1) in cultured human umbilical vein endothelial cells (HUVECs) via the AP-1 dependent mechanism and its enhanced secretion. In this work we provide evidence that the released PAI-1 is accumulated on the surface of HUVECs, exclusively in its active form, in a complex with alpha1-acid glycoprotein (AGP) that is also up-regulated and released from the cells. This mechanism is supported by several lines of experiments, in which expression of both proteins was analyzed by flow cytometry and their colocalization supported by confocal microscopy. PAI-1 did not bind to quiescent cells but only to the Tbeta4-activated endothelial cells. In contrast, significant amounts of AGP were found to be associated with the cells overexpressing enhanced green fluorescent protein (EGFP)-alpha1-acid glycoprotein (AGP) without Tbeta4 treatment. The AGP.PAI-1 complex was accumulated essentially at the basal surface of endothelial cells, and such cells showed (a) morphology characteristic for strongly adhered and spread cells and (b) significantly reduced plasmin formation. Taken together, these results provide the evidence supporting a novel mechanism by which active PAI-1 can be bound to the Tbeta4-activated endothelial cells, thus influencing their adhesive properties as well as their ability to generate plasmin.

  2. Three months of strictly controlled daily endurance exercise reduces thrombin generation and fibrinolytic risk markers in younger moderately overweight men

    DEFF Research Database (Denmark)

    Gram, Anne Sofie; Bladbjerg, Else-Marie; Skov, Jane;

    2015-01-01

    PURPOSE: Physical activity is associated with a decreased risk of cardiovascular disease, but dose dependency of long-term physical exercise on biomarkers within coagulation and fibrinolysis is unknown. We aimed to investigate effects of two doses of daily endurance exercise on biomarkers...

  3. Adaptive Augmenting Control Flight Characterization Experiment on an F/A-18

    Science.gov (United States)

    VanZwieten, Tannen S.; Orr, Jeb S.; Wall, John H.; Gilligan, Eric T.

    2014-01-01

    This paper summarizes the Adaptive Augmenting Control (AAC) flight characterization experiments performed using an F/A-18 (TN 853). AAC was designed and developed specifically for launch vehicles, and is currently part of the baseline autopilot design for NASA's Space Launch System (SLS). The scope covered here includes a brief overview of the algorithm (covered in more detail elsewhere), motivation and benefits of flight testing, top-level SLS flight test objectives, applicability of the F/A-18 as a platform for testing a launch vehicle control design, test cases designed to fully vet the AAC algorithm, flight test results, and conclusions regarding the functionality of AAC. The AAC algorithm developed at Marshall Space Flight Center is a forward loop gain multiplicative adaptive algorithm that modifies the total attitude control system gain in response to sensed model errors or undesirable parasitic mode resonances. The AAC algorithm provides the capability to improve or decrease performance by balancing attitude tracking with the mitigation of parasitic dynamics, such as control-structure interaction or servo-actuator limit cycles. In the case of the latter, if unmodeled or mismodeled parasitic dynamics are present that would otherwise result in a closed-loop instability or near instability, the adaptive controller decreases the total loop gain to reduce the interaction between these dynamics and the controller. This is in contrast to traditional adaptive control logic, which focuses on improving performance by increasing gain. The computationally simple AAC attitude control algorithm has stability properties that are reconcilable in the context of classical frequency-domain criteria (i.e., gain and phase margin). The algorithm assumes that the baseline attitude control design is well-tuned for a nominal trajectory and is designed to adapt only when necessary. Furthermore, the adaptation is attracted to the nominal design and adapts only on an as-needed basis

  4. An Experimental Study on a Microclimatic Layer of a Bionic Fa(c)ade Inspired by Vertical Greenery

    Institute of Scientific and Technical Information of China (English)

    Toma(z) (S)uklje; Sa(s)o Medved; Ciril Arkar

    2013-01-01

    A microclimatic layer of the green fa(c)ade is proven to have specific temperature and flow conditions on the building envelope.Lower temperatures and wind velocities,and higher relative humidity in the microclimatic layer are the characteristics of vertical greenery systems,which cause lower energy consumption for the cooling and heating of buildings.Despite innovative architectural solutions,there are some drawbacks to applying vertical greenery on building envelopes.In this study,a bionic fa(c)ade that mimics the positive effects and eliminates the disadvantages of green fa(c)ades is presented.The bionic fa(c)ade consists of bionic leaves,which are made of photovoltaic cells and evaporative matrices.A real scale experiment was carried out in the summer to evaluate the potential of the cooling efficiency of the microclimatic layer and a new photovoltaic cooling technique.The results show a good agreement of the thermal performance between the bionic and the green fa(c)ade and up to 20.8 K lower surface temperatures of photovoltaic cells,which increase the daily electricity yield by 6.6%.

  5. Interspecific hybridization does not affect the level of fluctuating asymmetry (FA) in the Drosophila bipectinata species complex.

    Science.gov (United States)

    Banerjee, Parul; Singh, B N

    2015-08-01

    The Drosophila bipectinata species complex comprises of four very closely related species namely D. bipectinata, D. parabipectinata, D. malerkotliana and D. pseudoananassae. It was found that irrespective of the evolutionary divergence among the species, FA which is reflective of the developmental precision remains nearly same in four species. During the present study, the level of FA in different morphological traits was studied in interspecific hybrids and compared with that of parental species with the view that it would throw light on the degree of divergence between the parental species. If they have not diverged much, the interspecific hybrids may have a similar FA level, incompatibilities between their genomes being negligible. On the other hand, if there is substantial divergence, the level of FA may be higher due to incompatibility between the genomes of the parental species. The morphological traits taken were sternopleural bristle number and wing length in both males and females and ovariole number and sex-comb tooth number in females and males respectively. However, except in a few cases, we could not detect any significant differences in the level of FA in hybrids as compared to pure species. On the other hand, a number of abnormalities like poor viability, dystrophied ovaries, asymmetrical eyes etc., could be detected in hybrids from crosses involving D. pseudoananassae as one of the parents. Therefore, we conclude that specific developmental pathways are more susceptible to developmental disturbances due to genomic incompatibilities than the large complex system bringing about developmental stability.

  6. Certification of Charpy V-Notch Reference Test Pieces of 30 J Nominal Absorbed Energy - Certified Reference Materials ERM®-FA013bg and ERM®-FA013bh

    OpenAIRE

    LAMBERTY MARIE ANDREE; Dean, Alan; Roebben, Gert

    2011-01-01

    This certification report describes the processing and characterisation of ERM®-FA013bg and ERM®-FA013bh, two batches of Charpy V-notch certified reference test pieces. Sets of five of these test pieces are used for the verification of pendulum impact test machines according to EN 10045-2 (Charpy impact test on metallic materials, Part 2. Method for the verification of impact testing machines [1]) or according to ISO 148-2 (Metallic materials - Charpy pendulum impact test - Part 2: Verificati...

  7. Cosa fa vivere gli uomini? di Lev Tolstoj. Anatomia di un successo

    Directory of Open Access Journals (Sweden)

    Damiano Rebecchini

    2010-12-01

    Full Text Available In questo studio ricostruiamo la storia di un racconto poco noto di Lev Tolstoj, Che cosa fa vivere gli uomini? (1881. In quest’opera Tolstoj volle rappresentare alcuni elementi chiave della sua nuova visione etico-religiosa maturata nel corso degli anni ’70 dell’Ottocento. Lo scrittore, inoltre, si impegnò nel distribuirla tra i ceti popolari urbani e nelle campagne e, grazie ad un’efficace opera di diffusione, l’opera fu letta da milioni di lettori popolari. Nell’articolo analizziamo da una parte le strategie narrative ed editoriali messe in gioco da Tolstoj nel suo racconto per conquistare i nuovi lettori; dall’altra, le tattiche di appropriazione, di adattamento e di trasformazione del testo tolstoiano da parte dei suoi lettori popolari.

  8. Operational safety and post-maintenance gas leak detection in GE frame 9001FA gas turbines

    Energy Technology Data Exchange (ETDEWEB)

    Farthing, D.A.; Marley, L.; Lees, J.A. [PowerGen plc, Connah' s Quay Power Station, Deeside (United Kingdom)

    1999-12-01

    The increased use of natural-gas-fuelled combined cycle gas turbine and combined heat and power plants in recent years has been accompanied by the practice of constructing acoustic enclosures around the gas turbines for environmental reasons. This can increase the risk of an explosion if, for any reason, there is a build-up of flammable gas within the enclosure, since there can be a range of possible ignition sources. The UK Health and Safety Executive consider gas leaks within such enclosures to be foreseeable, especially following maintenance. The paper presents a review of operational safety and a leak detection method for the inspection and design of flanged joints within the acoustic enclosures around GE 9001FA gas turbines, providing a practical step towards minimising the risk of explosion due to fuel leakage. (Author)

  9. Uncertainties in façade fire tests – measurements and modeling

    Directory of Open Access Journals (Sweden)

    Anderson Johan

    2016-01-01

    Full Text Available In this paper a comparison between test and modelling results are performed for two large-scale façade fire testing methods, namely SP Fire 105 and BS 8414-1. In order to be able to compare tests and modelling the uncertainties have to be quantified both in the test and the modelling. Here we present a methodology based on deterministic sampling to quantify uncertainties in the modelling input. We find, in general good agreement between the models and the test results. Moreover, temperatures estimated by plate thermometers is indicated to be less sensitive to small variations in model input and is thus suitable for these kind of comparisons.

  10. Comparing the performance of FA, DFA and DMA using different synthetic long-range correlated time series

    CERN Document Server

    Shao, Ying-Hui; Jiang, Zhi-Qiang; Zhou, Wei-Xing; Sornette, Didier

    2012-01-01

    Notwithstanding the significant efforts to develop estimators of long-range correlations (LRC) and to compare their performance, no clear consensus exists on what is the best method and under which conditions. In addition, synthetic tests suggest that the performance of LRC estimators varies when using different generators of LRC time series. Here, we compare the performances of four estimators [Fluctuation Analysis (FA), Detrended Fluctuation Analysis (DFA), Backward Detrending Moving Average (BDMA), and centred Detrending Moving Average (CDMA)]. We use three different generators [Fractional Gaussian Noises, and two ways of generating Fractional Brownian Motions]. We find that CDMA has the best performance and DFA is only slightly worse in some situations, while FA performs the worst. In addition, CDMA and DFA are less sensitive to the scaling range than FA. Hence, CDMA and DFA remain "The Methods of Choice" in determining the Hurst index of time series.

  11. Polarization of large cometary dust aggregates: computations with FaSTMM

    Science.gov (United States)

    Markkanen, Johannes; Penttilä, Antti; Muinonen, Karri

    2016-10-01

    We model light scattering by cometary dust with a special emphasis on polarization effects of large aggregated non-spherical dust particles. The contribution of large aggregates to the scattering characteristics of cometary dust is not well studied due to the rapid growth of computational time with respect to the number of grains in an aggregate when modelled by conventional numerical techniques (Mackowski, Mishchenko, JQSRT, 112(13), 2182-2192, 2011). To speed up computations, we apply our novel Fast Superposition T-Matrix Method (FaSTMM) that can deal with aggregates consisting of large numbers of non-spherical inhomogeneous grains.The FaSTMM is based on two individual solvers. First, the so-called T-matrices of the grains in an aggregate are determined by the method-of-moments solution of the Maxwell equations based on the volume-integral-equation approach. Once the T-matrices have been computed and stored, the second solver, i.e., the superposition T-matrix method, accelerated by the multilevel-fast-multipole algorithm (MLFMA), is employed to solve for the scattering properties of the entire aggregate consisting of arbitrarily rotated grains whose T-matrices had been computed by the first solver. Such an approach allows for efficient computations of ensample-averaged light-scattering features of aggregated dust particles. The MLFMA acceleration works especially well for sparse, fractal-like aggregates within a large volume. The method is exact in the sense that no approximation is made for the physics described by the classical electromagnetic scattering theory.As a dust model, we use porous aggregates with non-spherical grains consisting of a silicate core covered by organic refractory mantle. Thus, the dust particle model is consistent with the cometary dust formation model by Greenberg (Greenberg, Hage, Astrophys. J. 361, 260-274, 1990).Acknowledgments. Research supported by the European Research Council (ERC, grant Nr. 320773).

  12. Targeted delivery of Bcl-2 conversion gene by MPEG-PCL-PEI-FA cationic copolymer to combat therapeutic resistant cancer.

    Science.gov (United States)

    Li, Zibiao; Liu, Xuan; Chen, Xiaohong; Chua, Ming Xuan; Wu, Yun-Long

    2017-07-01

    Deregulation of anti-apoptosis Bcl-2 protein expression was a key feature in human cancers with therapeutic resistance. Nuclear receptor Nur77 could induce the conformation change of Bcl-2 protein and converted it into an apoptosis inducer by "enemy to friend" strategy. However, the safe and effective delivery of this gene to combat therapeutic resistant cancer remained largely unexplored. In this report, we designed an amphiphilic cationic MPEG-PCL-PEI-FA copolymer, comprising biocompatible and hydrophilic methoxy-poly(ethylene glycol) (MPEG), biodegradable and hydrophobic poly(ε-caprolactone) (PCL), cationic poly(ethylene imine) (PEI) segments, and folic acid (FA) as targeting group, as a high efficient Nur77 gene carrier to folate receptor (FR) highly expressed and therapeutic resistant HeLa/Bcl-2 cancer cells. Interestingly, due to the incorporation of PCL and PEG segments, this MPEG-PCL-PEI-FA copolymer showed less toxicity but better gene transfection efficiency than non-viral gene carrier gold standard PEI (25kDa). This might be due to the formation of micelles to stabilize polyplex for enhanced gene transfection ability. More importantly, MPEG-PCL-PEI-FA copolymer exhibited excellent growth inhibition ability on therapeutic resistant HeLa/Bcl-2 cancer cells, which was FR overexpressed HeLa cervical cancer cells with high expression of Bcl-2 protein, thanks to its FA induced targeting ability, high gene transfection efficiency, and low cytotoxicity. This work signifies the first time that cationic amphiphilic MPEG-PCL-PEI-FA copolymers could be utilized for the gene delivery to therapeutic resistant cancer cells with high expression of anti-apoptosis Bcl-2 protein and the positive results are encouraging for the further design of polymeric platforms for combating drug resistant tumors. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. 模拟咀嚼静态载荷nFA/PEEK牙种植体周应力分布特点%Distribution of Stress of nFA/PEEK Biomaterials in Dental Implant

    Institute of Scientific and Technical Information of China (English)

    甘露; 魏杰; 王冬梅; 马健

    2015-01-01

    Objective:To analyze distribution of stress surrounding poly-ether-ether-ketone bio-composited by nano-fluorap-atite (nFA/PEEK) dental implant under static loading. Methods:A 3-D mandible bone first molar region segment was mod-eled, nFA/PEEK and titanium alloy implants were established. Three-dimensional finite element analysis method was simu-lated to check distribution of stress under 180 N and 240 N static loadings, which were decomposed into vertical, buccolin-gual and mesiodistal directions. Results:Stress around implant abutment of buccolingual stress was higher than that of verti-cal and mesiodistal, which nFA/PEEK was 4.2 times lower than that of corresponding Ti 6Al4V. Stress of nFA/PEEK was con-centrated at the 1/3 neck area, which was less than Ti6Al4V. Stress of nFA/PEEK implant bone bed were located in 10 mm areas around the neck, which was higher than that of Ti6Al4V. Von-mises value calculated between 20~60 Mpa were found in a sorted as 240 N vertical Ti6Al4V>180 N mesiodistal or 240 buccolingual nFA/PEEK>240 N vertical Ti6Al4V>180 N buccol-ingual and 240 N nFA/PEEK>240 N vertical nFA/PEEK. Conclusion:nFA/PEEK in abutment is not easy to be broken un-der physiological masticatory force. A higher nFA/PEEK bone stress calculated between 20~60 MPa is reduced in extent of stress shielding effect, which is beneficial to cortical bone deposition and is potentially a promising implant material.%目的:纳米氟磷灰石聚醚醚酮(nFA/PEEK)是我国自主研发的复合种植体材料,本研究以三维有限元方法,分析该材料种植体静态载荷下应力分布特点. 方法:建立下颌第一磨牙区的局部骨块、nFA/PEEK和钛合金(Ti6Al4V)种植体全瓷冠修复的三维有限元模型,运用软件分析施力方向分别为垂直向、近远中向、颊舌向的180 N及240 N静态载荷下,对比2种材料种植体本身及其在骨内的应力分布状况. 结果:基台应力以颊舌向加载受力>垂直加载受力>近远中加载受力,nFA

  14. Parametric Optimization of Organic Rankine Cycle with R245fa/R601a as Working Fluid

    Institute of Scientific and Technical Information of China (English)

    Zhu Jialing; Kang Zhenhua; An Qingsong; Li Tailu

    2015-01-01

    In order to select the appropriate working fluids and optimize parameters for medium-temperature geothermally-powered organic Rankine cycle(ORC), R245fa is mixed with R601a atgeothermal water temperature of 110℃. Based on thermodynamics, the characteristics of mixture and its influence on the performance of ORC under different evaporating temperatures and composition proportionsare analyzed. Results show that the zeotropic mixture R245fa/R601a(0.4/0.6) has the highest performance. When the evaporating temperature reaches 67℃, the outlet temperature of geothermal water is 61℃, the net power output is the highest and the thermal efficiency is about 9%.

  15. Systems of Vegetal Façade and Green Roofs used as a Sustainable Option in Architecture

    OpenAIRE

    Chanampa, Mariana; Vidal Rivas, Pilar; Alonso Ojembarrena, Javier; Olivieri, Francesca

    2010-01-01

    Green architecture contributes not only in reducing the building’s thermal loads but also in reducing the effects of the urban heat island in densely built-up areas in a hardly natural environment. The current green systems are built in situ/on site and are very expensive, hence the need to create industrialized prevegetated systems which improve the buildings’ energy savings and reduce the times of construction works. The present paper describes three green systems for façades (gabion façade...

  16. Comparative Recovery of Microorganisms from BacT/ALERT Plastic and Glass FA and FN Blood Culture Bottles

    OpenAIRE

    Riley, J. A.; Heiter, B J; Bourbeau, P P

    2005-01-01

    bioMerieux, Inc., has recently introduced plastic bottles to replace glass bottles for use in the BacT/ALERT blood culture system. We compared the performance of the plastic to the glass bottles in a large clinical evaluation. Two blood cultures were collected from each patient, one using glass FA (aerobic) and FN (anaerobic) bottles and one using plastic FA and FN bottles. Of the 4,040 sets of four bottles collected, 3,110 contained the recommended 8 to 12 ml of blood, yielding 524 microorga...

  17. Distribution of medium-chain FA in different lipid classes after administration of specific structured TAG in rats

    DEFF Research Database (Denmark)

    Mu, Huiling; Høy, Carl-Erik

    2002-01-01

    -sn-glycerol (12:0/18:2/12:0). We conclude that the enterocyte has the ability to reacylate the MCFA into TAG and that the intestinal absorption of MCFA from STAG mainly occurs by resynthesis of TAG. Caprylic acid from STAG is not incorporated into phospholipids, whereas lauric acid from STAG can be incorporated......Structured TAG (STAG) containing medium-chain FA (MCFA) in the sn-1,3 positions and essential FA in the sn-2 position were synthesized by lipase-catalyzed acidolysis. In our previous studies we found that part of the MCFA from STAG could be absorbed in the small intestine; however, it was unclear...

  18. Dlk1/FA1 is a novel endocrine regulator of bone and fat mass and its serum level is modulated by growth hormone

    DEFF Research Database (Denmark)

    Abdallah, Basem; Ding, Ming; Jensen, Charlotte H

    2007-01-01

    Fat and bone metabolism are two linked processes regulated by several hormonal factors. Fetal antigen 1 (FA1) is the soluble form of dlk1 (delta-like 1), which is a member of the Notch-Delta family. We previously identified FA1 as a negative regulator of bone marrow mesenchymal stem cell...... differentiation. Here, we studied the effects of circulating FA1 on fat and bone mass in vivo by generating mice expressing high serum levels of FA1 (FA1 mice) using the hydrodynamic-based gene transfer procedure. We found that increased serum FA1 levels led to a significant reduction in total body weight, fat...... mass, and bone mass in a dose-dependent manner. Reduced bone mass in FA1 mice was associated with the inhibition of mineral apposition rate and bone formation rates by 58 and 72%, respectively. Because FA1 is colocalized with GH in the pituitary gland, we explored the possible modulation of serum FA1...

  19. Controlled clinical comparison of plastic and glass bottles of BacT/ALERT FA medium for culturing organisms from blood of adult patients.

    Science.gov (United States)

    Petti, Cathy A; Mirrett, Stanley; Woods, Christopher W; Reller, L Barth

    2005-04-01

    A new, clear-plastic nonvented aerobic FA bottle, designed to prevent breakage, has been developed for the BacT/ALERT blood culture system. We assessed the new plastic FA bottle by comparing its performance with that of the current glass FA bottle for recovery of microorganisms and time to detection of growth in blood samples obtained for culture from adult patients with suspected bloodstream infections. We conclude that the BacT/ALERT plastic and glass FA bottles are comparable for recovery of microorganisms and that the safety advantage of plastic bottles can be achieved without compromising performance.

  20. Controlled Clinical Comparison of Plastic and Glass Bottles of BacT/ALERT FA Medium for Culturing Organisms from Blood of Adult Patients

    OpenAIRE

    Petti, Cathy A.; Mirrett, Stanley; Woods, Christopher W.; Reller, L. Barth

    2005-01-01

    A new, clear-plastic nonvented aerobic FA bottle, designed to prevent breakage, has been developed for the BacT/ALERT blood culture system. We assessed the new plastic FA bottle by comparing its performance with that of the current glass FA bottle for recovery of microorganisms and time to detection of growth in blood samples obtained for culture from adult patients with suspected bloodstream infections. We conclude that the BacT/ALERT plastic and glass FA bottles are comparable for recovery ...

  1. The cleaner, the greener? Product sustainability assessment of the biomimetic façade paint Lotusan® in comparison to the conventional façade paint Jumbosil®

    Directory of Open Access Journals (Sweden)

    Florian Antony

    2016-12-01

    Full Text Available Background: The debate on the question whether biomimetics has a specific potential to contribute to sustainability is discussed among scientists, business leaders, politicians and those responsible for project funding. The objective of this paper is to contribute to this controversial debate by presenting the sustainability assessment of one of the most well-known and most successful biomimetic products: the façade paint Lotusan®.Results: As a first step it has been examined and verified that the façade paint Lotusan® is correctly defined as a biomimetic product. Secondly, Lotusan® has been assessed and compared to a conventional façade paint within the course of a detailed product sustainability assessment (PROSA. For purposes of comparison, the façade paint Jumbosil® was chosen as reference for a conventional paint available on the market. The benefit analysis showed that both paints fulfil equally well the requirements of functional utility. With respect to the symbolic utility, Lotusan® has a particular added aesthetic value by the preservation of the optical quality over the life cycle. Within the social analysis no substantial differences between the two paints could be found regarding the handling and disposal of the final products. Regarding the life-cycle cost, Lotusan® is the more expensive product. However, the higher investment cost for a Lotusan®-based façade painting are more than compensated by the longer life time, resulting in both reduced overall material demand and lower labour cost. In terms of the life-cycle impact assessment, it can be ascertained that substantial differences between the paints arise from the respective service life, which are presented in terms of four scenario analyses.Conclusion: In summary, the biomimetic façade paint Lotusan® has been identified as a cost-effective and at the same time resource-saving product. Based on the underlying data and assumptions it could be demonstrated that

  2. Neurons in the monoaminergic nuclei of the rat and human central nervous system express FA1/dlk

    DEFF Research Database (Denmark)

    Jensen, Charlotte Harken; Meyer, M; Schrøder, Henrik Daa

    2001-01-01

    The gene DLK1 encodes a member of the epidermal growth factor (EGF) superfamily, delta-like (dlk). When exposed in vivo to the action of an unknown protease, this type 1 membrane protein generates a soluble peptide referred to as Fetal antigen 1 (FA1). By acting in juxtacrine as well as paracrine...

  3. Production Support Flight Control Computers: Research Capability for F/A-18 Aircraft at Dryden Flight Research Center

    Science.gov (United States)

    Carter, John F.

    1997-01-01

    NASA Dryden Flight Research Center (DFRC) is working with the United States Navy to complete ground testing and initiate flight testing of a modified set of F/A-18 flight control computers. The Production Support Flight Control Computers (PSFCC) can give any fleet F/A-18 airplane an in-flight, pilot-selectable research control law capability. NASA DFRC can efficiently flight test the PSFCC for the following four reasons: (1) Six F/A-18 chase aircraft are available which could be used with the PSFCC; (2) An F/A-18 processor-in-the-loop simulation exists for validation testing; (3) The expertise has been developed in programming the research processor in the PSFCC; and (4) A well-defined process has been established for clearing flight control research projects for flight. This report presents a functional description of the PSFCC. Descriptions of the NASA DFRC facilities, PSFCC verification and validation process, and planned PSFCC projects are also provided.

  4. A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development.

    Directory of Open Access Journals (Sweden)

    Philip J Byrd

    2016-03-01

    Full Text Available Patients with biallelic truncating mutations in PALB2 have a severe form of Fanconi anaemia (FA-N, with a predisposition for developing embryonal-type tumours in infancy. Here we describe two unusual patients from a single family, carrying biallelic PALB2 mutations, one truncating, c.1676_1677delAAinsG;(p.Gln559ArgfsTer2, and the second, c.2586+1G>A; p.Thr839_Lys862del resulting in an in frame skip of exon 6 (24 amino acids. Strikingly, the affected individuals did not exhibit the severe developmental defects typical of FA-N patients and initially presented with B cell non-Hodgkin lymphoma. The expressed p.Thr839_Lys862del mutant PALB2 protein retained the ability to interact with BRCA2, previously unreported in FA-N patients. There was also a large increased chromosomal radiosensitivity following irradiation in G2 and increased sensitivity to mitomycin C. Although patient cells were unable to form Rad51 foci following exposure to either DNA damaging agent, U2OS cells, in which the mutant PALB2 with in frame skip of exon 6 was induced, did show recruitment of Rad51 to foci following damage. We conclude that a very mild form of FA-N exists arising from a hypomorphic PALB2 allele.

  5. The effect of playing formation on high-intensity running and technical profiles in English FA Premier League soccer matches

    DEFF Research Database (Denmark)

    Bradley, Paul S; Carling, Chris; Archer, Dave

    2011-01-01

    The aim of this study was to examine the effect of playing formation on high-intensity running and technical performance during elite soccer matches. Twenty English FA Premier League games were analysed using a multiple-camera computerized tracking system (n = 153 players). Overall ball possessio...

  6. Safety and efficacy of subcutaneous tocilizumab in adults with systemic sclerosis (faSScinate) : a phase 2, randomised, controlled trial

    NARCIS (Netherlands)

    Khanna, Dinesh; Denton, Christopher P.; Jahreis, Angelika; van Laar, Jacob M.; Frech, Tracy M.; Anderson, Marina E.; Baron, Murray; Chung, Lorinda; Fierlbeck, Gerhard; Lakshminarayanan, Santhanam; Allanore, Yannick; Pope, Janet E.; Riemekasten, Gabriela; Steen, Virginia; Müller-Ladner, Ulf; Lafyatis, Robert; Stifano, Giuseppina; Spotswood, Helen; Chen-Harris, Haiyin; Dziadek, Sebastian; Morimoto, Alyssa; Sornasse, Thierry; Siegel, Jeffrey; Furst, Daniel E.

    2016-01-01

    Background Systemic sclerosis is a rare disabling autoimmune disease with few treatment options. The efficacy and safety of tocilizumab, an interleukin 6 receptor-α inhibitor, was assessed in the faSScinate phase 2 trial in patients with systemic sclerosis. Methods We did this double-blind, placebo-

  7. FA1 immunoreactivity in endocrine tumours and during development of the human fetal pancreas; negative correlation with glucagon expression

    DEFF Research Database (Denmark)

    Tornehave, D; Jensen, Charlotte Harken; Teisner, B

    1996-01-01

    Fetal antigen 1 (FA1) is a glycoprotein containing six epidermal growth factor (EGF)-like repeats. It is closely similar to the protein translated from the human delta-like (dlk) cDNA and probably constitutes a proteolytically processed form of dlk. dlk is homologous to the Drosophila homeotic pr...

  8. 梅毒螺旋体金属蛋白酶水解纤维蛋白原的活性研究%The Fibrinolytic Potential of Treponema Pallidum Metalloprotease

    Institute of Scientific and Technical Information of China (English)

    刘双全

    2015-01-01

    Objective To investigate the fibrinolytic potential of Treponema pallidum metalloprotease Tp0751,provi-ding the experimental basis for further research on syphilis pathogenic mechanism. Methods Constructed the H198A mutant Tp0751 and H202A mutant Tp0751 with two-point technology. Wide-type and Mutant Tp0751 recombinant protein were expressed in E. coli BL21. And detected their degradation activity to fibrinogen. Results Three fusion protein with molecular weight about 26KDa were attained after expression and purification,The wild type Tp0751 can degradate plas-minogen-free human fibrinogen,fibrinogen was absolutely degradated 24h post-incubation,The H198A mutant Tp0751 can also degradate plasminogen-free human fibrinogen,but which was partly degradated 24h post-incubation;The H202A mutant Tp0751 can not degradate plasminogen-free human fibrinogen,fibrinogen was scarcely degradated 24h post-incubation. Conclusion The wild type Tp0751 can degradate plasminogen-free human fibrinogen,The HEXXH motif may have a im-portant role on Tp0751 to the degradation of plasminogen-free human fibrinogen,residue H202,but not H198,from the palli-lysin HEXXH motif is required for fibrinogen degradation.%目的:研究梅毒螺旋体金属蛋白酶Tp0751对细胞外基质纤维蛋白原的水解能力,为深入研究TP的致病机制提供实验依据。方法采用两点法构建H198A突变型及H202A突变型Tp0751基因,分别构建野生型和突变型Tp0751原核载体进行诱导表达;体外实验检测Tp0751野生型Tp0751蛋白、H198A Tp0751突变型蛋白、H202ATp0751突变型蛋白对纤维蛋白原的降解活性。结果成功表达并纯化了野生型Tp0751蛋白、H198A Tp0751突变体蛋白、H202A Tp0751突变体蛋白,各蛋白相对分子量大小约为26kD;纤维蛋白原在与野生型Tp0751蛋白作用约24h后被完全水解;与 H198A Tp0751突变体蛋白作用约24 h 后部分水解,尚有部分未被降解;与H202A Tp0751

  9. Environmental and health effects of nanomaterials in nanotextiles and façade coatings.

    Science.gov (United States)

    Som, Claudia; Wick, Peter; Krug, Harald; Nowack, Bernd

    2011-08-01

    Engineered nanomaterials (ENM) are expected to hold considerable potential for products that offer improved or novel functionalities. For example, nanotechnologies could open the way for the use of textile products outside their traditional fields of applications, for example, in the construction, medical, automobile, environmental and safety technology sectors. Consequently, nanotextiles could become ubiquitous in industrial and consumer products in future. Another ubiquitous field of application for ENM is façade coatings. The environment and human health could be affected by unintended release of ENM from these products. The product life cycle and the product design determine the various environmental and health exposure situations. For example, ENM unintentionally released from geotextiles will probably end up in soils, whereas ENM unintentionally released from T-shirts may come into direct contact with humans and end up in wastewater. In this paper we have assessed the state of the art of ENM effects on the environment and human health on the basis of selected environmental and nanotoxicological studies and on our own environmental exposure modeling studies. Here, we focused on ENM that are already applied or may be applied in future to textile products and façade coatings. These ENM's are mainly nanosilver (nano-Ag), nano titanium dioxide (nano-TiO(2)), nano silica (nano-SiO(2)), nano zinc oxide (nano-ZnO), nano alumina (nano-Al(2)O(3)), layered silica (e.g. montmorillonite, Al(2)[(OH)(2)/Si(4)O(10)]nH(2)O), carbon black, and carbon nanotubes (CNT). Knowing full well that innovators have to take decisions today, we have presented some criteria that should be useful in systematically analyzing and interpreting the state of the art on the effects of ENM. For the environment we established the following criteria: (1) the indication for hazardous effects, (2) dissolution in water increases/decreases toxic effects, (3) tendency for agglomeration or sedimentation

  10. Current Status on Mechanical Disassembly and Shearing System Development in FaCT Project

    Energy Technology Data Exchange (ETDEWEB)

    Washiya, T.; Tasaka, M.; Kitagaki, T.; Higuti, H.; Koizumi, K.; Myouchin, M.; Koyama, T. [JAEA, 4-33 Muramatsu, Tokai-mura, Ibaraki 319-1194 (Japan); Kobayashi, T. [Japan Atomic Power Company - JAPC (Japan)

    2009-06-15

    Japan Atomic Energy Agency (JAEA) has been carried out the Fast Reactor Cycle Technology Development (FaCT) project in cooperation with the Japanese electric utilities. As for the development of the advanced aqueous reprocessing in the FaCT project, JAEA and Japan Atomic Power Company (JAPC) have been developing a reliable disassembly and shearing system for FBR fuel reprocessing. As configuration of FBR fuel assembly, fuel pins are covered with a hexagonal shaped wrapper tube, which must be separated from the fuel pins prior to the shear process. We proposed new disassembly procedures combined two mechanical cutting operations and one pulling operation. The cutting operation has two modes, such as the slit-cutting mode for the wrapper tube cutting and the crop-cutting mode for the pin bundle cutting to separate the entrance nozzle. After the slit-cut operations, the wrapper tube will be pulled and removed from the fuel pins bundle. Some fundamental experiments were carried out to select the cutting tool by using FMS and ODS steals nominated as the material of the commercial FBR fuel, and CBN wheel shown the highest durability and selected as the promising tool. With reflecting of these technologies, an engineering-scale system testing device was designed and fabricated. And to confirm the system performance, some demonstration tests by using a simulated fuel assembly of prototype reactor 'Monju' has been stared. In the fuel shearing process, we proposed the short-length shearing to obtain highly fragmented fuel to provide efficient fuel dissolution, which required adapting to the uranium crystallization process. The fragment rate is affected by the shear-length and the layout of fuel pins in the shear magazine. In order to optimize the shear conditions, parametric tests by using shear machine and simulated fuel pins was carried out. As the results, optimum shear length is selected to be 10 mm. In this paper, we will report the latest experimental results

  11. Psychometric Properties of the Fatigue Questionnaire EORTC QLQ-FA12 in a sample of female cancer patients.

    Science.gov (United States)

    Kecke, Sophie; Ernst, Jochen; Einenkel, Jens; Singer, Susanne; Hinz, Andreas

    2017-08-11

    . Cancer patients frequently suffer from fatigue. Recently, the European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life group developed a new 12-item fatigue assessment instrument. The aim of this study was to psychometrically test this questionnaire in comparison with the 3-item fatigue scale of the EORTC QLQ-C30. A sample of 354 patients who were being treated for breast cancer or gynaecological cancer were examined using the new fatigue questionnaire EORTC QLQ-FA12 and the EORTC QLQ-C30 during their hospital stay (t1) and three months after hospital discharge (t2). Confirmatory factorial analyses (CFA), item analyses, test-retest reliability analyses, and correlation analyses were performed. The analyses roughly supported the three-factorial structure of the FA12, which is comprised of the subscales Physical, Emotional, and Cognitive fatigue. The fit indices of the CFA were worse than those of the original paper but nevertheless acceptable. Cronbach's alpha of the total scale was 0.92; the coefficients of the subscales were between 0.79 and 0.93. The correlation between the EORTC QLQ-FA12 total scale and the fatigue scale of the EORTC QLQ-C30 was 0.69, the correlation between the t1 and t2 scores was 0.45 for the EORTC QLQ-FA12 total scale and between 0.37 and 0.47 for the subscales. The psychometric coefficients justify the calculation of a sum score, which can be used by clinicians to assess the general degree of fatigue. While the 3-item fatigue scale of the EORTC QLQ-C30 stresses the physical aspect of fatigue, the new EORTC QLQ-FA12 covers its emotional and cognitive aspects as well. Copyright © 2017. Published by Elsevier Inc.

  12. Comparative recovery of microorganisms from BacT/ALERT plastic and glass FA and FN blood culture bottles.

    Science.gov (United States)

    Riley, J A; Heiter, B J; Bourbeau, P P

    2005-07-01

    bioMerieux, Inc., has recently introduced plastic bottles to replace glass bottles for use in the BacT/ALERT blood culture system. We compared the performance of the plastic to the glass bottles in a large clinical evaluation. Two blood cultures were collected from each patient, one using glass FA (aerobic) and FN (anaerobic) bottles and one using plastic FA and FN bottles. Of the 4,040 sets of four bottles collected, 3,110 contained the recommended 8 to 12 ml of blood, yielding 524 microorganisms with 359 judged to be clinically significant. Of the 359 significant organisms, 255 were recovered in either one or two bottles from both pairs of bottles in a set while 56 organisms were recovered only from the glass bottles and 48 were recovered only from the plastic bottles (P, not significant [NS]). Of the 286 significant organisms recovered only in the FA bottles (glass and plastic), 180 were recovered in both bottles, 57 in the plastic bottles only, and 49 in the glass bottles only (P, NS). Of the 303 significant organisms recovered in the FN bottles only (glass and plastic), 212 were recovered in both bottles, 46 in the plastic bottles only, and 45 in the glass bottles only (P, NS). For individual organisms, the only significant difference in recovery was obtained for Escherichia coli, with more isolates recovered in the FN plastic than in the FN glass bottles (P = 0.02). These data suggest that recovery of microorganisms with plastic FA/FN bottles is at least equal to that with glass FA/FN bottles while offering greater safety for users.

  13. Targeted Treatment of Nasopharyngeal Carcinoma with FA-CM-β-CD-CDDP Nanomedicine%FA-CM-β-CD-CDDP 纳米药物对鼻咽癌的体内靶向治疗研究

    Institute of Scientific and Technical Information of China (English)

    刘涛; 候雄军; 伍锡栋; 柯波; 王蓉; 刘素珍

    2015-01-01

    ABSTRACT:Objective To investigate the effect of folic acid(FA)-targeted cisplatin(CDDP)con-jugated carboxymethyl-β-cyclodextrin(CM-β-CD)nanomedicine(FA-CM-β-CD-CDDP)on naso-pharyngeal carcinoma(NPC)in vivo.Methods Nude mice bearing folate receptor(FR)-positive HNE-1 tumor and FR-negative CNE-2 tumor were given FA-CM-β-CD-CDDP nanomedicine(CD-DP 10 mg·kg-1 )via tail vein injection.The concentrations of CDDP in tumor tissues were deter-mined 1.5 hours after injection by using atomic absorption spectroscopy to analyze the targeting ability of FA-CM-β-CD-CDDP.Twenty nude mice bearing HNE-1 tumor were randomly given normal saline(control group,n=5),FA-CM-β-CD solution(vector group,n=5),3 mg·kg-1 CD-DP solution(CDDP group,n=5)or 3 mg/kg FA-CM-β-CD-CDDP solution(nanomedicine group, n=5)at a volume of 2 ml via tail vein injection.HNE-1 tumor volume and weight were measured 21 days after administration.Furthermore,the expression of proliferating cell nuclear antigen(PC-NA)was detected by immunohistochemistry to analyze the efficacy of FA-CM-β-CD-CDDP for tumor suppression.Results The concentration of CDDP in HNE-1 tumor was significantly higher than that in CNE-2 tumor(P <0.05).The inhibition rates of HNE-1 tumor volume and weight in nanomedicine group(43.36% and 41.67%,respectively)were significantly higher than those in vector group(7.91% and 5.56%,respectively)and CDDP group(24.51% and 22.22%,respec-tively).Moreover,positive PCNA index in HNE-1 tumor in nanomedicine group(47.75%)was significantly lower than that in control group(90.83%),vector group(89.62%)and CDDP group (68.43%).Conclusion The constructed A-CM-β-CD-CDDP nanomedicine can inhibit the growth of FR-positive NPC tumor.%目的:探讨叶酸(folic acid,FA)分子靶向载顺铂(cisplatin,CDDP)羧甲基-β-环糊精(carboxymethyl-β-cyclo-dextrin,CM-β-CD)纳米药物(FA-CM-β-CD-CDDP)对鼻咽癌(nasopharyngeal carcinoma,NPC)的体内靶向治疗效应

  14. Development of a Façade Assessment and Design Tool for Solar Energy (FASSADES

    Directory of Open Access Journals (Sweden)

    Jouri Kanters

    2014-03-01

    Full Text Available Planning energy-efficient buildings which produce on-site renewable energy in an urban context is a challenge for all involved actors in the planning process. The primary objective of this study was to develop a façade assessment and design tool for solar energy (FASSADES providing the necessary information for all stakeholders in the design process. The secondary objective was to demonstrate the tool by performing an assessment analysis of a building block. The FASSADES tool is a DIVA4Rhino script, combining Radiance/Daysim and EnergyPlus for simulating the annual production of solar thermal and photovoltaic systems on facades, the cost-effectiveness of the solar energy system, and the payback time. Different output methods are available; graphically within the 3D drawing environment and numerically within post-processing software. The tool was tested to analyse a building block within a city under Swedish conditions. Output of the developed tool showed that shading from nearby buildings greatly affects the feasibility of photovoltaic and solar thermal systems on facades.

  15. Comparison of thermal testing of MS9001FA type GTPs at shatura and nizhnevartovsk GRES

    Science.gov (United States)

    Ol'khovskii, G. G.

    2016-11-01

    Domestic power plants use combined-cycle plants in which a gas-turbine plant (GTP) and a steam turbine rotate a common electric generator. In this instance, it is impossible to measure the power of each of them, so we have to resort to some assumptions. We have succeeded to check the validity of these assumptions and possible errors of their application testing combined-cycle plants (CCP) with the same GTP and a steam turbine but operating each on its own electrical generator. Comparative tests of a MS901FA GTP of the PGU-400 power-generating unit commissioned at Shatura GRES (a thermal power station) and a GTP of the same type installed at Nizhnevartovsk GRES were performed. As a result of these tests, dependences of the electric power of both gas-turbine plants and a turbine outlet temperature on the inlet temperature were obtained. A relation of the GTP efficiency, heat and air rate on the load are determined, and characteristics of compressors and turbines of both GTPs are defined. The performed tests have confirmed the accuracy of the determined characteristics of the two GTPs using both a direct measurement of net power (Nizhnevartovsk GRES) and an indirect measurement (Shatura GRES).

  16. Cyberlindnera tropicalis f.a., sp. nov., a novel yeast isolated from tropical regions.

    Science.gov (United States)

    Boontham, Wanatchaporn; Limtong, Savitree; Rosa, Carlos A; Lopes, Mariana R; Vital, Marcos J S; Srisuk, Nantana

    2017-08-01

    Two yeast strains, DMKU-WBBC14 and UFMG-CM-Y3283, were isolated from soil in Samutprakarn province in the central part of Thailand and from rotting wood in an Amazonian forest site in the Roraima State in Brazil, respectively. The two strains showed identical sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene and different sequences by three nucleotide substitutions of the internal transcribed spacer (ITS) region. Therefore, these two strains represented a single species which was most closely related to Cyberlindnera mengyuniae CBS 10845T. The nucleotide sequence differences between the two strains of the novel species and the type strain Cyberlindnera mengyuniae CBS 10845T were 10 substitutions in the D1/D2 region of the LSU rRNA gene and 46 substitutions in the ITS region. DMKU-WBBC14 and UFMG-CM-Y3283 differed in growth temperature profiles. Moreover, they also exhibited different carbon assimilation profiles and growth temperature profiles from the type strain of Cyberlindnera mengyuniae, CBS 10845T. The name Cyberlindnera tropicalis f.a., sp. nov. is proposed. The type strain is DMKU-WBBC14T (=CBS 14558T=TBRC 6562T). The Mycobank number is MB 819722.

  17. Effects of crowd size on referee decisions: analysis of the FA Cup.

    Science.gov (United States)

    Downward, Paul; Jones, Marc

    2007-12-01

    Data were collected on the number of first yellow cards awarded during 857 games, over six seasons (1996 - 2002), played in the Football Association (FA) Cup. Overall, a significantly higher number of yellow cards were awarded against the away team, while a non-linear relationship between crowd size and yellow cards was observed. In general, the probability of a yellow card being awarded against the home team decreased as crowd size increased, but was attenuated for the largest crowd sizes. Crowd size may be related to the probability of the home team receiving a yellow card in two potential ways. Crowd noise may be a decision-making heuristic whereby the likelihood that an incident is a foul is increased when accompanied by crowd noise. Alternatively, referees may seek to appease the crowd and are more likely to do so as crowd size increases. The present findings have implications for the training of match officials and for coaches and players as they prepare to play away from home.

  18. Adaptive Augmenting Control Flight Characterization Experiment on an F/A-18

    Science.gov (United States)

    VanZwieten, Tannen S.; Gilligan, Eric T.; Wall, John H.; Orr, Jeb S.; Miller, Christopher J.; Hanson, Curtis E.

    2014-01-01

    The NASA Marshall Space Flight Center (MSFC) Flight Mechanics and Analysis Division developed an Adaptive Augmenting Control (AAC) algorithm for launch vehicles that improves robustness and performance by adapting an otherwise welltuned classical control algorithm to unexpected environments or variations in vehicle dynamics. This AAC algorithm is currently part of the baseline design for the SLS Flight Control System (FCS), but prior to this series of research flights it was the only component of the autopilot design that had not been flight tested. The Space Launch System (SLS) flight software prototype, including the adaptive component, was recently tested on a piloted aircraft at Dryden Flight Research Center (DFRC) which has the capability to achieve a high level of dynamic similarity to a launch vehicle. Scenarios for the flight test campaign were designed specifically to evaluate the AAC algorithm to ensure that it is able to achieve the expected performance improvements with no adverse impacts in nominal or nearnominal scenarios. Having completed the recent series of flight characterization experiments on DFRC's F/A-18, the AAC algorithm's capability, robustness, and reproducibility, have been successfully demonstrated. Thus, the entire SLS control architecture has been successfully flight tested in a relevant environment. This has increased NASA's confidence that the autopilot design is ready to fly on the SLS Block I vehicle and will exceed the performance of previous architectures.

  19. Proteasome activity is important for replication recovery, CHK1 phosphorylation and prevention of G2 arrest after low-dose formaldehyde

    Energy Technology Data Exchange (ETDEWEB)

    Ortega-Atienza, Sara; Green, Samantha E.; Zhitkovich, Anatoly, E-mail: anatoly_zhitkovich@brown.edu

    2015-07-15

    Formaldehyde (FA) is a human carcinogen with numerous sources of environmental and occupational exposures. This reactive aldehyde is also produced endogenously during metabolism of drugs and other processes. DNA–protein crosslinks (DPCs) are considered to be the main genotoxic lesions for FA. Accumulating evidence suggests that DPC repair in high eukaryotes involves proteolysis of crosslinked proteins. Here, we examined a role of the main cellular proteolytic machinery proteasomes in toxic responses of human lung cells to low FA doses. We found that transient inhibition of proteasome activity increased cytotoxicity and diminished clonogenic viability of FA-treated cells. Proteasome inactivation exacerbated suppressive effects of FA on DNA replication and increased the levels of the genotoxic stress marker γ-H2AX in normal human cells. A transient loss of proteasome activity in FA-exposed cells also caused delayed perturbations of cell cycle, which included G2 arrest and a depletion of S-phase populations at FA doses that had no effects in control cells. Proteasome activity diminished p53-Ser15 phosphorylation but was important for FA-induced CHK1 phosphorylation, which is a biochemical marker of DPC proteolysis in replicating cells. Unlike FA, proteasome inhibition had no effect on cell survival and CHK1 phosphorylation by the non-DPC replication stressor hydroxyurea. Overall, we obtained evidence for the importance of proteasomes in protection of human cells against biologically relevant doses of FA. Biochemically, our findings indicate the involvement of proteasomes in proteolytic repair of DPC, which removes replication blockage by these highly bulky lesions. - Highlights: • Proteasome inhibition enhances cytotoxicity of low-dose FA in human lung cells. • Active proteasomes diminish replication-inhibiting effects of FA. • Proteasome activity prevents delayed G2 arrest in FA-treated cells. • Proteasome inhibition exacerbates replication stress by FA in

  20. Orientations of Iron-Sulfur Clusters FA and FB in the Homodimeric Type-I Photosynthetic Reaction Center of Heliobacterium modesticaldum.

    Science.gov (United States)

    Kondo, Toru; Matsuoka, Masahiro; Azai, Chihiro; Itoh, Shigeru; Oh-Oka, Hirozo

    2016-05-12

    Orientations of the FA and FB iron-sulfur (FeS) clusters in a structure-unknown type-I homodimeric heriobacterial reaction center (hRC) were studied in oriented membranes of the thermophilic anaerobic photosynthetic bacterium Heliobacterium modesticaldum by electron paramagnetic resonance (EPR), and compared with those in heterodimeric photosystem I (PS I). The Rieske-type FeS center in the cytochrome b/c complex showed a well-oriented EPR signal. Illumination at 14 K induced an FB(-) signal with g-axes of gz = 2.066, gy = 1.937, and gx = 1.890, tilted at angles of 60°, 60°, and 45°, respectively, with respect to the membrane normal. Chemical reduction with dithionite produced an additional signal of FA(-), which magnetically interacted with FB(-), with gz = 2.046, gy = 1.942, and gx = 1.911 at 30°, 60°, and 90°, respectively. The angles and redox properties of FA(-) and FB(-) in hRC resemble those of FB(-) and FA(-), respectively, in PS I. Therefore, FA and FB in hRC, named after their g-value similarities, seem to be located like FB and FA, not like FA and FB, respectively, in PS I. The reducing side of hRC could resemble those in PS I, if the names of FA and FB are interchanged with each other.

  1. Dlk1/FA1 Is a Novel Endocrine Regulator of Bone and Fat Mass and Its Serum Level Is Modulated By Growth Hormone

    DEFF Research Database (Denmark)

    Abdallah, B.M.; Ding, M.; Jensen, C.H.

    2007-01-01

    Fat and bone metabolism are two linked processes regulated by several hormonal factors. FA1 (fetal antigen 1) is the soluble form of dlk1 (delta like 1), which is a member of the Notch-Delta family. We have previously identified FA1 as a negative regulator of bone marrow mesenchymal stem cell...

  2. The use of matrigel has no influence on tumor development or PET imaging in FaDu human head and neck cancer xenografts

    DEFF Research Database (Denmark)

    Fliedner, Frederikke P.; Hansen, Anders Elias; Jorgensen, Jesper T.

    2016-01-01

    is currently available. This study evaluates the potential effect of matrigel use in a human head and neck cancer xenograft model (FaDu; hypopharyngeal carcinoma) in NMRI nude mice. The FaDu cell line was chosen based on its frequent use in studies of cancer imaging and tumor microenvironment. Methods: NMRI...

  3. Human serum levels of fetal antigen 1 (FA1/Dlk1) increase with obesity, are negatively associated with insulin sensitivity and modulate inflammation in vitro

    DEFF Research Database (Denmark)

    Chacón, M R; Miranda, M; Jensen, C H

    2008-01-01

    of Insulin Resistance), cytokines (sIL-6), adipokines (adiponectin) and circulating soluble fractions of tumor necrosis factor-alpha receptors 1 and 2 (sTNFR1 and sTNFR2). RESULTS: IN the obesity study, levels of FA1 in serum were found to increase with obesity. The S(i) index was negatively dependent on FA1...

  4. Quantitative diffusion tensor MR imaging of the brain: field strength related variance of apparent diffusion coefficient (ADC) and fractional anisotropy (FA) scalars.

    Science.gov (United States)

    Huisman, Thierry A G M; Loenneker, Thomas; Barta, Gerd; Bellemann, Matthias E; Hennig, Juergen; Fischer, Joachim E; Il'yasov, Kamil A

    2006-08-01

    The objectives were to study the "impact" of the magnetic field strength on diffusion tensor imaging (DTI) metrics and also to determine whether magnetic-field-related differences in T2-relaxation times of brain tissue influence DTI measurements. DTI was performed on 12 healthy volunteers at 1.5 and 3.0 Tesla (within 2 h) using identical DTI scan parameters. Apparent diffusion coefficient (ADC) and fractional anisotropy (FA) values were measured at multiple gray and white matter locations. ADC and FA values were compared and analyzed for statistically significant differences. In addition, DTI measurements were performed at different echo times (TE) for both field strengths. ADC values for gray and white matter were statistically significantly lower at 3.0 Tesla compared with 1.5 Tesla (% change between -1.94% and -9.79%). FA values were statistically significantly higher at 3.0 Tesla compared with 1.5 Tesla (% change between +4.04 and 11.15%). ADC and FA values are not significantly different for TE=91 ms and TE=125 ms. Thus, ADC and FA values vary with the used field strength. Comparative clinical studies using ADC or FA values should consequently compare ADC or FA results with normative ADC or FA values that have been determined for the field strength used.

  5. Delta-like 1/fetal antigen 1(DLK1/FA1) inhibits BMP2 induced osteoblast differentiation through modulation of NFκB signaling pathway

    DEFF Research Database (Denmark)

    Qiu, Weimin; Abdallah, Basem; Kassem, Moustapha

    DLK1/FA1 (delta-like 1/fetal antigen-1) is a negative regulator of bone mass that acts to inhibit osteoblast differentiation and stimulate osteoclast differentiation. However, the molecular mechanisms underlying these effects are not known. Thus, we studied the effect of DLK1/FA1 on different...

  6. Electrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa Rats

    Directory of Open Access Journals (Sweden)

    Claudia S. Maier

    2013-08-01

    Full Text Available This study reports on the use of traveling wave ion mobility quadrupole time-of-flight (ToF mass spectrometry for plasma metabolomics. Plasma metabolite profiles of obese Zucker fa/fa rats were obtained after the administration of different oral doses of Xanthohumol; a hop-derived dietary supplement. Liquid chromatography coupled data independent tandem mass spectrometry (LC-MSE and LC-ion mobility spectrometry (IMS-MSE acquisitions were conducted in both positive and negative modes using a Synapt G2 High Definition Mass Spectrometry (HDMS instrument. This method provides identification of metabolite classes in rat plasma using parallel alternating low energy and high energy collision spectral acquisition modes. Data sets were analyzed using pattern recognition methods. Statistically significant (p 1.5 features were selected to identify the up-/down-regulated metabolite classes. Ion mobility data visualized using drift scope software provided a graphical read-out of differences in metabolite classes.

  7. Defective homing is associated with altered Cdc42 activity in cells from patients with Fanconi anemia group A

    Science.gov (United States)

    Zhang, Xiaoling; Shang, Xun; Guo, Fukun; Murphy, Kim; Kirby, Michelle; Kelly, Patrick; Reeves, Lilith; Smith, Franklin O.; Williams, David A.

    2008-01-01

    Previous studies showed that Fanconi anemia (FA) murine stem cells have defective reconstitution after bone marrow (BM) transplantation. The mechanism underlying this defect is not known. Here, we report defective homing of FA patient BM progenitors transplanted into mouse models. Using cells from patients carrying mutations in FA complementation group A (FA-A), we show that when transplanted into nonobese diabetic/severe combined immunodeficiency (NOD/SCID) recipient mice, FA-A BM cells exhibited impaired homing activity. FA-A cells also showed defects in both cell-cell and cell-matrix adhesion. Complementation of FA-A deficiency by reexpression of FANCA readily restored adhesion of FA-A cells. A significant decrease in the activity of the Rho GTPase Cdc42 was found associated with these defective functions in patient-derived cells, and expression of a constitutively active Cdc42 mutant was able to rescue the adhesion defect of FA-A cells. These results provide the first evidence that FA proteins influence human BM progenitor homing and adhesion via the small GTPase Cdc42-regulated signaling pathway. PMID:18565850

  8. P(FA-co-MMA-co-St)共混改性环氧涂料的制备与疏水性能%Preparation and Hydrophotic Property of Copolymers P(FA-Co-MMA-co-St) Modified Epoxy Coatings

    Institute of Scientific and Technical Information of China (English)

    王月欣; 李景红; 张倩; 王善兴; 郝文珺

    2011-01-01

    The superhydrophobic epoxy coatings were prepared by adding the random copolymers poly-(fluoroalkyl ethyl acrylate-co-methly methacrylate-co-styrene) P(FA-co-MMA-co-St) to the commercially available epoxy paints using a simple blending method. The results indicated that when the copolymers are 4% of the epoxy components,perfluoroalkyl ethyl acrylate (FA) accounts for 30% of the comonomers, curing temperature is 120 ℃/2h, the water contact angle(WCA) of epoxy coatings is 152°, higher than 88° of unmodified epoxy coatings. Through scanning electron microscope(SEM), the microstructure was observed on the epoxy coatings. The low surface energy FA segrnents and the microstructure together renderes the modified epoxy coatings with superhydrophobic properties.The factors were studied as well.%采用简单的共混法,直接将聚全氟烷基乙基丙烯酸酯-co-甲基丙烯酸甲酯-co-苯乙烯无规共聚物P(FA-co-MMA-co-St)添加到市售的环氧涂料中,制备出具有超疏水性的环氧涂层.结果表明,当共聚物用量为环氧色漆组分的4%,氟单体投料量为30%,固化剂为乙二醇双偏苯三酸酐(TMEG)、固化温度120℃,固化时间2h条件下,改性环氧涂层对水的接触角可达152°,比纯的环氧涂层提高了88°.电子扫描显微镜观察发现,在环氧涂层表面构建出了具有微米级的粗糙结构.

  9. Occurrence of Far-Red Light Photoacclimation (FaRLiP in Diverse Cyanobacteria

    Directory of Open Access Journals (Sweden)

    Fei Gan

    2014-12-01

    Full Text Available Cyanobacteria have evolved a number of acclimation strategies to sense and respond to changing nutrient and light conditions. Leptolyngbya sp. JSC-1 was recently shown to photoacclimate to far-red light by extensively remodeling its photosystem (PS I, PS II and phycobilisome complexes, thereby gaining the ability to grow in far-red light. A 21-gene photosynthetic gene cluster (rfpA/B/C, apcA2/B2/D2/E2/D3, psbA3/D3/C2/B2/ H2/A4, psaA2/B2/L2/I2/F2/J2 that is specifically expressed in far-red light encodes the core subunits of the three major photosynthetic complexes. The growth responses to far-red light were studied here for five additional cyanobacterial strains, each of which has a gene cluster similar to that in Leptolyngbya sp. JSC-1. After acclimation all five strains could grow continuously in far-red light. Under these growth conditions each strain synthesizes chlorophylls d, f and a after photoacclimation, and each strain produces modified forms of PS I, PS II (and phycobiliproteins that absorb light between 700 and 800 nm. We conclude that these photosynthetic gene clusters are diagnostic of the capacity to photoacclimate to and grow in far-red light. Given the diversity of terrestrial environments from which these cyanobacteria were isolated, it is likely that FaRLiP plays an important role in optimizing photosynthesis in terrestrial environments.

  10. Does plasmin have anticoagulant activity?

    Directory of Open Access Journals (Sweden)

    Jane Hoover-Plow

    2010-03-01

    Full Text Available Jane Hoover-PlowJoseph J Jacobs Center for Thrombosis and Vascular Biology, Departments of Cardiovascular Medicine and Molecular Cardiology, Lerner Research Institute Cleveland Clinic, Ohio, USAAbstract: The coagulation and fibrinolytic pathways regulate hemostasis and thrombosis, and an imbalance in these pathways may result in pathologic hemophilia or thrombosis. The plasminogen system is the primary proteolytic pathway for fibrinolysis, but also has important proteolytic functions in cell migration, extracellular matrix degradation, metalloproteinase activation, and hormone processing. Several studies have demonstrated plasmin cleavage and inactivation of several coagulation factors, suggesting plasmin may be not only be the primary fibrinolytic enzyme, but may have anticoagulant properties as well. The objective of this review is to examine both in vitro and in vivo evidence for plasmin inactivation of coagulation, and to consider whether plasmin may act as a physiological regulator of coagulation. While several studies have demonstrated strong evidence for plasmin cleavage and inactivation of coagulation factors FV, FVIII, FIX, and FX in vitro, in vivo evidence is lacking for a physiologic role for plasmin as an anticoagulant. However, inactivation of coagulation factors by plasmin may be useful as a localized anticoagulant therapy or as a combined thrombolytic and anticoagulant therapy.Keywords: thrombosis, anticoagulant, cardiovascular disease, plasminogen’s protease, blood

  11. Purification and characterization of tenerplasminin-1, a serine peptidase inhibitor with antiplasmin activity from the coral snake (Micrurus tener tener) venom.

    Science.gov (United States)

    Vivas, Jeilyn; Ibarra, Carlos; Salazar, Ana M; Neves-Ferreira, Ana G C; Sánchez, Elda E; Perales, Jonás; Rodríguez-Acosta, Alexis; Guerrero, Belsy

    2016-01-01

    A plasmin inhibitor, named tenerplasminin-1 (TP1), was isolated from Micrurus tener tener (Mtt) venom. It showed a molecular mass of 6542Da, similarly to Kunitz-type serine peptidase inhibitors. The amidolytic activity of plasmin (0.5nM) on synthetic substrate S-2251 was inhibited by 91% following the incubation with TP1 (1nM). Aprotinin (2nM) used as the positive control of inhibition, reduced the plasmin amidolytic activity by 71%. Plasmin fibrinolytic activity (0.05nM) was inhibited by 67% following incubation with TP1 (0.1nM). The degradation of fibrinogen chains induced by plasmin, trypsin or elastase was inhibited by TP1 at a 1:2, 1:4 and 1:20 enzyme:inhibitor ratio, respectively. On the other hand, the proteolytic activity of crude Mtt venom on fibrinogen chains, previously attributed to metallopeptidases, was not abolished by TP1. The tPA-clot lysis assay showed that TP1 (0.2nM) acts like aprotinin (0.4nM) inducing a delay in lysis time and lysis rate which may be associated with the inhibition of plasmin generated from the endogenous plasminogen activation. TP1 is the first serine protease plasmin-like inhibitor isolated from Mtt snake venom which has been characterized in relation to its mechanism of action, formation of a plasmin:TP1 complex and therapeutic potential as anti-fibrinolytic agent, a biological characteristic of great interest in the field of biomedical research. They could be used to regulate the fibrinolytic system in pathologies such as metastatic cancer, parasitic infections, hemophilia and other hemorrhagic syndromes, in which an intense fibrinolytic activity is observed.

  12. Modeling a Naturally Ventilated Double Skin Façade with a Building Thermal Simulation Program

    DEFF Research Database (Denmark)

    Jensen, Rasmus Lund; Kalyanova, Olena; Heiselberg, Per

    2008-01-01

    The use of Double Skin Façade (DSF) has increased during the last decade. There are many reasons for this including e.g. aesthetics, sound insulation, improved indoor environment and energy savings. However, the influence on the indoor environment and energy consumption are very difficult...... to predict. This is manly due to the very transient and complex air flow in the naturally ventilated double skin façade cavity. In this paper the modelling of the DSF using a thermal simulation program, BSim, is discussed. The simulations are based on the measured weather boundary conditions......, and the simulation results are compared to the measurement results like energy consumption for cooling, air temperature, temperature gradient and mass flow rate in the DSF cavity, etc. Details about the measurements are reported in \\Kalyanova et al. 2008\\. The thermal simulation program does not at the moment...

  13. FA Café 世界上最好吃的红虾奖得主比萨

    Institute of Scientific and Technical Information of China (English)

    米糖

    2014-01-01

    2014年9月,位于新光天地购物中心5楼的美食区正式对外营业,而这个区域的亮点之一就是FA Café。FA Café即Fissler Academy Café,它由制作大师ENZO COCCIA携手菲仕乐带来比萨发源地意大利那不勒斯正宗原味,烹调出曾荣获红虾奖的世界上最好吃的手工比萨,同时呈现各种绝妙地中海美食。

  14. Cloning of strawberry FaEtr2 gene and its plant expression vector construction for antisense RNA

    Institute of Scientific and Technical Information of China (English)

    Chunli SONG; Junlian MA; Xia TANG; Zide ZHANG; Pingping ZHOU; Zhixia HOU

    2009-01-01

    An ethylene receptor FaEtr2 gene was amplified by polymerase chain reaction (PCR) from ripening strawberry fruit. A 1049-bp PCR product (All Star-Etr2) was cloned. Sequence analysis showed that the All Star-Etr2 nucleotide sequence had 100% identity with Chandler-Etr2 from the GenBank. A pair of primers containing restriction enzyme sites were designed and used to amplify the sequenced plasmid. The PCR product was digested by the corresponding restricted enzymes and inserted between the CaMV 35S promoter and NOS terminator of expression vector pBI121 directionally. The constructed expression vector was transformed into Agrobacterium fumefeciens LBA4404 in the follow-up research to silence a ripening-related ethylene receptor FaEtr2 gene in strawberry fruits.

  15. Optimizing Client Latency in a Distributed System by Using the “Remote Façade” Design Pattern

    Directory of Open Access Journals (Sweden)

    Cosmin RABLOU

    2010-01-01

    Full Text Available In this paper we investigate the role of the Remote Façade pattern in the optimization of dis-tributed systems. The intent of this pattern is to wrap fine-grained remote objects in a coarse-grained interface and thus greatly reduce the total number of calls executed over the network. The measurement of the performance gain achieved by implementing this pattern is done through testing with a distributed application written in C# and using the latest Microsoft framework for distributed systems (Windows Communication Framework. Furthermore, we will be presenting the scenarios in which the implementation of the Remote Façade pattern brings a significant performance gain. Finally we show further scenarios in which the per-formance brought by this pattern can be investigated.

  16. International Psychometric Validation of an EORTC Quality of Life Module Measuring Cancer Related Fatigue (EORTC QLQ-FA12).

    Science.gov (United States)

    Weis, Joachim; Tomaszewski, Krzysztof A; Hammerlid, Eva; Ignacio Arraras, Juan; Conroy, Thierry; Lanceley, Anne; Schmidt, Heike; Wirtz, Markus; Singer, Susanne; Pinto, Monica; Alm El-Din, Mohamed; Compter, Inge; Holzner, Bernhard; Hofmeister, Dirk; Chie, Wei-Chu; Czeladzki, Marek; Harle, Amelie; Jones, Louise; Ritter, Sabrina; Flechtner, Hans-Henning; Bottomley, Andrew

    2017-05-01

    The European Organisation for Research and Treatment of Cancer (EORTC) Group has developed a new multidimensional instrument measuring cancer-related fatigue to be used in conjunction with the quality of life core questionnaire (EORTC QLQ-C30). The module EORTC QLQ-FA13 assesses physical, cognitive, and emotional aspects of cancer-related fatigue. The methodology follows the EORTC guidelines for phase IV validation of modules. This paper focuses on the results of the psychometric validation of the factorial structure of the module. For validation and cross-validation confirmatory factor analysis (maximum likelihood estimation), intraclass correlation and Cronbach alpha for internal consistency were employed. The study involved an international multicenter collaboration of 11 European and non-European countries. A total of 946 patients with various tumor diagnoses were enrolled. Based on the confirmatory factor analysis, we could approve the three-dimensional structure of the module. Removing one item and reassigning the factorial mapping of another item resulted in the EORTC QLQ-FA12. For the revised scale, we found evidence supporting good local (indicator reliability ≥ 0.60, factor reliability ≥ 0.82) and global model fit (GFI t1|t2 = 0.965/0.957, CFI t1|t2 = 0.976/0.972, RMSEA t1|t2 = 0.060/0.069) for both measurement points. For each scale, test-retest reliability proved to be very good (intraclass correlation: R t1-t2 = 0.905-0.921) and internal consistency proved to be good to high (Cronbach alpha = .79-.90). Based on the former phase III module, the multidimensional structure was revised as a phase IV module (EORTC FA12) with an improved scale structure. For a comprehensive validation of the EORTC FA12, further aspects of convergent and divergent validity as well as sensitivity to change should be determined.

  17. Which support technology can give to mathematics formative assessment? : The FaSMEd project in Italy and France.

    OpenAIRE

    Aldon, Gilles; cusi, analisa; Morselli, Francesca; Panero, Monica; Sabena, Cristina

    2015-01-01

    International audience; This workshop is focused on the role technology may play in supporting the formative assessment process. Different examples from the case studies developed in France and Italy within the European Project FaSMEd will be analysedand discussed. In order to highlight the choices we made in relation to the aim of the project, before discussing the examples we will introduce the methodology adopted in each country and the theoretical frameworks to which we refer for the plan...

  18. Curcumin reverses cisplatin resistance in cisplatin-resistant lung caner cells by inhibiting FA/BRCA pathway.

    Science.gov (United States)

    Chen, Ping; Li, Jian; Jiang, He-Guo; Lan, Ting; Chen, Yong-Chang

    2015-05-01

    Cisplatin (DDP) is the most widely used chemotherapy agent for treatment of malignancies including lung cancer. However, the effectiveness of DDP is often weakened by acquired resistance of tumor cells. DDP kills cancer cells primarily by creating intrastrand and interstrand DNA cross-links, which block DNA replication. The Fanconi anemia (FA)/BRCA pathway is a DNA cross-link damage repair pathway, which regulates cellular resistance to DNA cross-link agents, such as DDP. Some study has shown that natural compound curcumin sensitize human ovarian and breast cancer cells to DDP. However, whether curcumin may reverse resistance to DDP in DDP-resistant lung cancer cells has not been understood. In this study, we showed that curcumin enhanced the proliferation inhibitory effect of DDP and promote DDP-induced apoptosis in A549/DDP cells (DDP-resistant lung adenocarcinoma cells). Moreover, we observed that FA/BRCA pathway DNA damage repair processes, such as DDP-induced FANCD2 monoubiquitination and nuclear foci formation were downregulated in the presence of curcumin, suggesting that curcumin enhanced sensitivity to DDP in A549/DDP cells through the inhibition of FA/BRCA pathway. Furthermore, the calculation of q value and apoptosis analyses revealed that curcumin in combination with DDP could exert a synergistic cytotoxic effect in A549/DDP cells, further demonstrating that curcumin can reverse cisplatin resistance of A549/DDP cells. In conclusion, by suppressing the FA/BRCA pathway DNA repair, curcumin potentiates DDP-induced proliferation inhibitory effect and apoptosis in A549/DDP cell, indicating that curcumin may serve as a chemosensitizer to cross-link-inducing anticancer drugs DDP.

  19. Segmentation of Façades from Urban 3D Point Clouds Using Geometrical and Morphological Attribute-Based Operators

    Directory of Open Access Journals (Sweden)

    Andrés Serna

    2016-01-01

    Full Text Available 3D building segmentation is an important research issue in the remote sensing community with relevant applications to urban modeling, cloud-to-cloud and cloud-to-model registration, 3D cartography, virtual reality, cultural heritage documentation, among others. In this paper, we propose automatic, parametric and robust approaches to segment façades from 3D point clouds. Processing is carried out using elevation images and 3D decomposition, and the final result can be reprojected onto the 3D point cloud for visualization or evaluation purposes. Our methods are based on geometrical and geodesic constraints. Parameters are related to urban and architectural constraints. Thus, they can be set up to manage façades of any height, length and elongation. We propose two methods based on façade marker extraction and a third method without markers based on the maximal elongation image. This work is developed in the framework of TerraMobilita project. The performance of our methods is proved in our experiments on TerraMobilita databases using 2D and 3D ground truth annotations.

  20. A water tunnel flow visualization study of the vortex flow structures on the F/A-18 aircraft

    Science.gov (United States)

    Sandlin, Doral R.; Ramirez, Edgar J.

    1991-01-01

    The vortex flow structures occurring on the F/A-18 aircraft at high angles of attack were studied. A water tunnel was used to gather flow visualization data on the forebody vortex and the wing leading edge extension vortex. The longitudinal location of breakdown of the leading edge vortex was found to be consistently dependent on the angle of attack. Other parameters such as Reynolds number, model scale, and model fidelity had little influence on the overall behavior of the flow structures studied. The lateral location of the forebody vortex system was greatly influenced by changes in the angle of sideslip. Strong interactions can occur between the leading edge extension vortex and the forebody vortex. Close attention was paid to vortex induced flows on various airframe components of the F/A-18. Reynolds number and angle of attack greatly affected the swirling intensity, and therefore the strength of the studied vortices. Water tunnel results on the F/A-18 correlated well with those obtained in similar studies at both full and sub scale levels. The water tunnel can provide, under certain conditions, good simulations of realistic flows in full scale configurations.

  1. Comparative Study on Acoustic Behavior Between Light Multi-layered and Traditional Façades

    Directory of Open Access Journals (Sweden)

    Ruiz, L.

    2012-09-01

    Full Text Available Light multi-layered facade systems in general have been acoustically little studied. The data available suggests they do not usually fulfil the sound insulation values required in the Technical Building Code (CTE in particularly in noisy environments. Therefore the main objectives of this study, is to obtain light multi-layered façade systems with a high degree of acoustic insulation that can be used in noisy environments. While in turn showing excellent thermal characteristics, without neglecting important aspects such as the sustainability of materials, industrialization, costs, among others. This article will explain the process that has been followed to carry out an investigation on the acoustic behaviour of light multi-layered facades. For this purpose acoustic behaviour and sound intensimetry measurement methods have been used. After obtaining these results, a comparison between light multi-layered and traditional facades was made.Los sistemas de fachadas multicapas ligeras acústicamente, en general han sido poco estudiados. Los primeros datos que se tienen es que no suelen alcanzar los valores de aislamiento acústico requeridos en el Código Técnico de la Edificación (CTE en entornos especialmente ruidosos. Los objetivos principales de esta investigación son obtener fachadas multicapas ligeras con un alto grado de aislamiento acústico, que puedan ser empleados en entornos ruidosos. Al mismo tiempo que presenten excelentes características térmicas, sin descuidar aspectos tan importantes como la sostenibilidad, industrialización, costes, etc. En este artículo se explicará el proceso que se ha llevado a cabo para realizar una investigación relacionada con el comportamiento acústico de fachadas multicapas ligeras. Para ello se han empleado los métodos de medida del comportamiento acústico a ruido aéreo e intensimetría sonora. Una vez obtenidos estos resultados se realiza una comparativa entre las fachadas multicapas ligeras

  2. Molecular advances in plasminogen activator inhibitor 1 interaction with thrombin and tissue-type plasminogen activator.

    Science.gov (United States)

    Stoop, A; van Meijer, M; Horrevoets, A J; Pannekoek, H

    1997-02-01

    Plasminogen activator inhibitor 1 (PAI-1) is a glycoprotein that controls the activity of the key enzymes of the fibrinolytic system, the serine proteases tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). Inhibition is accomplished by rapid formation of inactive, equimolar PAI-1/PA complexes. The physiological importance of PAI-1 for the fibrinolytic system has been underscored by the observation that in humans, a homozygous defect results in hemorrhagic episodes. In addition to its function in surveillance of the integrity of clots, PAI-1 efficiently inhibits the serine protease thrombin in vitro, provided that either the high molecular weight glycosaminoglycan heparin or the glycoprotein vitronectin is present. These cofactors accelerate the rate of thrombin inhibition by PAI-1 by more than two orders of magnitude. Inhibition of thrombin by PAI-1 proceeds according to a "suicide substrate mechanism," typified by a branched reaction pathway, leading either to stable PAI-1/thrombin complexes or to degradation of the inhibitor and recycling of enzyme. The cofactors heparin and vitronectin, although increasing inhibition through different mechanisms, essentially promote PAI-1 degradation by thrombin. In view of the multitude of functions attributed to thrombin, the authors propose that the relevance of thrombin inhibition by PAI-1 is to restrict its mitogenic activity, rather than to affect its coagulation function in plasma. (Trends Cardiovasc Med 1997;7:47-51). © 1997, Elsevier Science Inc.

  3. Decipher the dynamic coordination between enzymatic activity and structural modulation at focal adhesions in living cells

    Science.gov (United States)

    Lu, Shaoying; Seong, Jihye; Wang, Yi; Chang, Shiou-Chi; Eichorst, John Paul; Ouyang, Mingxing; Li, Julie Y.-S.; Chien, Shu; Wang, Yingxiao

    2014-07-01

    Focal adhesions (FAs) are dynamic subcellular structures crucial for cell adhesion, migration and differentiation. It remains an enigma how enzymatic activities in these local complexes regulate their structural remodeling in live cells. Utilizing biosensors based on fluorescence resonance energy transfer (FRET), we developed a correlative FRET imaging microscopy (CFIM) approach to quantitatively analyze the subcellular coordination between the enzymatic Src activation and the structural FA disassembly. CFIM reveals that the Src kinase activity only within the microdomain of lipid rafts at the plasma membrane is coupled with FA dynamics. FA disassembly at cell periphery was linearly dependent on this raft-localized Src activity, although cells displayed heterogeneous levels of response to stimulation. Within lipid rafts, the time delay between Src activation and FA disassembly was 1.2 min in cells seeded on low fibronectin concentration ([FN]) and 4.3 min in cells on high [FN]. CFIM further showed that the level of Src-FA coupling, as well as the time delay, was regulated by cell-matrix interactions, as a tight enzyme-structure coupling occurred in FA populations mediated by integrin αvβ3, but not in those by integrin α5β1. Therefore, different FA subpopulations have distinctive regulation mechanisms between their local kinase activity and structural FA dynamics.

  4. Factors V and VII anticoagulant activities in the salivary glands of feeding Dermacentor andersoni ticks.

    Science.gov (United States)

    Gordon, J R; Allen, J R

    1991-02-01

    The salivary glands of Dermacentor andersoni ticks possess anticoagulant activities that can alter the clotting time of rabbit whole blood. Salivary gland extracts from female ticks inhibit both the intrinsic and extrinsic coagulation systems, and maximal activities against both pathways occur when the ticks attain about 250 mg feeding weight. These anticoagulants are directed against both coagulation factors V and VII, but they do not affect factors II or X. Despite this salivary anticoagulant activity, heavily tick-infested rabbits suffer no visible alteration of their peripheral blood coagulability and have no detectable circulating fibrin degradation products, suggesting that the ticks do not secrete a factor with fibrinolytic activity.

  5. Study on flame retardant properties of FA and EVA/FA composites synergistic with red phosphorus%粉煤灰及其与红磷复配阻燃EVA复合材料的性能

    Institute of Scientific and Technical Information of China (English)

    钱翌; 李少权

    2016-01-01

    Ethylene-vinyl acetate(EVA)/fly-ash(FA)/red phosphorus(RP)composites were prepared by EVA as resin matrix,FA as flame retardant composites and RP as synergist. The combustion and smoke generation of the composites were studied by limiting oxygen index(LOI)test, cone calorimeter test(CCT) and smoke density test(SDT) as well as the mechanism of flame retardant and smoke suppression. The results show that LOI value of the composites is the highest with the mass ratio of EVA, FA and RP is50∶47∶3, which reaches25.6%. The heat release rate, mass loss, smoke production rate of the composites are decreased significantly compared with those of pure EVA, which represent excellent flame retardant properties. The composites reflects good smoke suppression performance under the ignition and non-ignition conditions.%以乙烯-乙酸乙烯共聚物(EVA)为基体树脂,粉煤灰(FA)为复配阻燃剂,通过添加协效阻燃剂红磷(RP)制得EVA/FA/RP复合材料。采用极限氧指数(LOI)测试、锥形量热仪测试、烟密度测试等研究了复合材料燃烧性能和生烟性能,并探讨了其阻燃及抑烟机理。结果表明:w(EVA)为50%,w(FA)为47%,w(RP)为3%时,制备的复合材料的LOI最高,达到了25.6%;与纯EVA相比,复合材料的热释放速率、质量损失、烟生成速率均显著降低,表现出良好的阻燃性能;在点火和未点火情况下,复合材料均表现出良好的抑烟性能。

  6. Mn(ii) mediated degradation of artemisinin based on Fe3O4@MnSiO3-FA nanospheres for cancer therapy in vivo

    Science.gov (United States)

    Chen, Jian; Zhang, Weijie; Zhang, Min; Guo, Zhen; Wang, Haibao; He, Mengni; Xu, Pengping; Zhou, Jiajia; Liu, Zhenbang; Chen, Qianwang

    2015-07-01

    Artemisinin (ART) is a natural drug with potent anticancer activities related with Fe2+ mediated cleavage of the endoperoxide bridge in ART. Herein, we reported that Mn2+ could substitute for Fe2+ to react with ART and generate toxic products, inducing a much higher anticancer efficiency. On this basis, we prepared pH-responsive Fe3O4@MnSiO3-FA nanospheres which can efficiently deliver hydrophobic ART into tumors in mice models. Mn2+ was released in acidic tumor environments and intracellular lysosomes, interacting with ART to kill cancer cells. The ART-loaded nanocarriers could suppress tumor growth more efficiently than free ART, which could be further illustrated by magnetic resonance imaging (MRI). Histological analysis revealed that the drug delivery system had no obvious effect on the major organs of mice. ART has been reported to have lower toxicity than chemotherapeutics. The ART-loaded nanocarriers are promising to be used in improving the survival of chemotherapy patients, providing a novel method for clinical tumor therapy.Artemisinin (ART) is a natural drug with potent anticancer activities related with Fe2+ mediated cleavage of the endoperoxide bridge in ART. Herein, we reported that Mn2+ could substitute for Fe2+ to react with ART and generate toxic products, inducing a much higher anticancer efficiency. On this basis, we prepared pH-responsive Fe3O4@MnSiO3-FA nanospheres which can efficiently deliver hydrophobic ART into tumors in mice models. Mn2+ was released in acidic tumor environments and intracellular lysosomes, interacting with ART to kill cancer cells. The ART-loaded nanocarriers could suppress tumor growth more efficiently than free ART, which could be further illustrated by magnetic resonance imaging (MRI). Histological analysis revealed that the drug delivery system had no obvious effect on the major organs of mice. ART has been reported to have lower toxicity than chemotherapeutics. The ART-loaded nanocarriers are promising to be used in

  7. Ações de fomento à pesquisa científica na FaBCI/FESPSP: panorama do período de 2008 a 2010Actions of scientific development research at FaBCI/FESPSP: overview of the period 2008 to 2010

    Directory of Open Access Journals (Sweden)

    Wellington Ferreira Rodrigues

    2012-06-01

    Full Text Available Apresenta e descreve as ações de incentivo à pesquisa científica desenvolvidas na Fundação Escola de Sociologia e Política de São Paulo - FESPSP entre os anos de 2008 e 2010. O foco do trabalho é a participação dos alunos da Faculdade de Biblioteconomia e Ciência da Informação – FaBCI nestes programas e a produção apresentada nestes anos. Além disso, são apresentados os resultados obtidos pelo programa de Monitoria Científica, aplicado em 2010 apenas no curso de Biblioteconomia e Ciência da Informação da FESPSP, entre os meses de abril a dezembro de 2010. Palavras-chave: Pesquisa científica. Iniciação científica. Instituição de Ensino Superior. Abstract This paper presents and describes activities to encourage scientific research developed at Fundação Escola de Sociologia e Política de São Paulo (FESPSP between 2008 and 2010. The main focus of this study is the participation of students of the School of Library and Information Science (FaBCI in these programs and their production throughout these years. Moreover, we present the results of the program of Scientific Monitoring, applied exclusively in the course of Library and Information Science of FESPSP between April and December 2010. Keywords: Scientific research. Undergraduate research. College Education Institution.

  8. The strawberry (Fragariaxananassa) fruit-specific rhamnogalacturonate lyase 1 (FaRGLyase1) gene encodes an enzyme involved in the degradation of cell-wall middle lamellae.

    Science.gov (United States)

    Molina-Hidalgo, Francisco J; Franco, Antonio R; Villatoro, Carmen; Medina-Puche, Laura; Mercado, José A; Hidalgo, Miguel A; Monfort, Amparo; Caballero, José Luis; Muñoz-Blanco, Juan; Blanco-Portales, Rosario

    2013-04-01

    Pectins are essential components of primary plant cell walls and middle lamellae, and are related to the consistency of the fruit and its textural changes during ripening. In fact, strawberries become soft as the middle lamellae of cortical parenchyma cells are extensively degraded during ripening, leading to the observed short post-harvest shelf life. Using a custom-made oligonucleotide-based strawberry microarray platform, a putative rhamnogalacturonate lyase gene (FaRGlyase1) was identified. Bioinformatic analysis of the FaRGlyase1 sequence allowed the identification of a conserved rhamnogalacturonate lyase domain, which was also present in other putative RGlyase sequences deposited in the databases. Expression of FaRGlyase1 occurred mainly in the receptacle, concurrently with ripening, and it was positively regulated by abscisic acid and negatively by auxins. FaRGLyase1 gene expression was transiently silenced by injecting live Agrobacterium cells harbouring RNA interference constructs into fruit receptacles. Light and electron microscopy analyses of these transiently silenced fruits revealed that this gene is involved in the degradation of pectins present in the middle lamella region between parenchymatic cells. In addition, genetic linkage association analyses in a strawberry-segregating population showed that FaRGLyase1 is linked to a quantitative trait loci linkage group related to fruit hardness and firmness. The results showed that FaRGlyase1 could play an important role in the fruit ripening-related softening process that reduces strawberry firmness and post-harvest life.

  9. Potent in vitro synergism of fusidic acid (FA) and berberine chloride (BBR) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA).

    Science.gov (United States)

    Liang, Rong-mei; Yong, Xiao-lan; Duan, Yu-qin; Tan, Yong-hong; Zeng, Ping; Zhou, Zi-ying; Jiang, Yan; Wang, Shi-hua; Jiang, Yun-ping; Huang, Xiao-chun; Dong, Zhao-hui; Hu, Ting-ting; Shi, Hui-qing; Li, Nan

    2014-11-01

    It was found in the present study that combined use of fusidic acid (FA) and berberine chloride (BBR) offered an in vitro synergistic action against 7 of the 30 clinical methicillin-resistant Staphylococcus aureus (MRSA) strains, with a fractional inhibitory concentration (FIC) index ranging from 0.5 to 0.19. This synergistic effect was most pronounced on MRSA 4806, an FA-resistant isolate, with a minimum inhibitory concentration (MIC) value of 1,024 μg/ml. The time-kill curve experiment showed that FA plus BBR yielded a 4.2 log10 c.f.u./ml reduction in the number of MRSA 4806 bacteria after 24-h incubation as compared with BBR alone. Viable count analysis showed that FA plus BBR produced a 3.0 log10 c.f.u./ml decrease in biofilm formation and a 1.5 log10 c.f.u./ml decrease in mature biofilm in viable cell density as compared with BBR alone. In addition, phase contrast micrographs confirmed that biofilm formation was significantly inhibited and mature biofilm was obviously destructed when FA was used in combination with BBR. These results provide evidence that combined use of FA and BBR may prove to be a promising clinical therapeutic strategy against MRSA.

  10. Clinical comparison of the Bactec Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN blood culture vials for the detection of candidemia.

    Science.gov (United States)

    Ericson, Eva-Lena; Klingspor, Lena; Ullberg, Måns; Ozenci, Volkan

    2012-06-01

    The present study analyzed the performance of Bactec Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN vials in detection and time to detection (TTD) of Candida spp. in 179 simultaneous blood cultures. The Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN vials could detect Candida spp. in 144 (80.45%) of 179, 149 (83.24%) of 179, and 8 (4.47%) of 179 samples, respectively. With the presence of antifungal therapy, the numbers of positive vials were higher in BacT/Alert FA compared to Mycosis IC/F, 87/99 versus 73/99, respectively (P IC/F than in BacT/Alert FA vials without antifungal therapy, 20.89 (9.33) versus 28.26 (9.77), respectively (P IC/F than in BacT/Alert FA vials, 28/30 and 19/30, respectively (P = 0.01). The present data show that the use of Bactec Mycosis IC/F together with BacT/Alert FA vials might improve the detection of Candida spp.

  11. Derivation of elastic stiffness formula for leaf type HDS and conceptual design of leaf type HDS of SMART FA

    Energy Technology Data Exchange (ETDEWEB)

    Song, Kee Nam; Kang, Heung Seok; Yoon, Kyung Ho; Suh, Jung Min; Lee, Jin Seok

    1997-12-01

    Based on the strain energy method and Euler beam theory, an elastic stiffness formula for the leaf type HDS, now widely used as the holddown spring for the FA of Westinghouse type PWRs, has been derived. Through comparisons with the characteristic test results of the test produced HDSs, it has been found that the derived formula is useful to reliably estimate an elastic stiffness with material properties and the geometric data of an HDS. Through sensitivity analysis of HDS`s elastic stiffness, the elastic stiffness sensitivity with respect to different design variables was identified, as well as the design variables having remarkable sensitivity. In addition, finite element analysis using surface-to-surface contact elements on the contact surface between the leaves shows that the analysis results are in good agreement with the elastic stiffness determined from the derived formula. It is therefore expected that the finite element model and the analysis method will be useful in the analysis of the elasto-plastic behavior of the leaf type HDS in the future. To both reduce the cobalt content, which is considered to be the source of radioactive contamination in the reactor core, and to design the HDS to meet the holddown requirements of the SMART FA, a conceptual design for the HDS of the SMART FA has been performed through two analyses of the elastic characteristics of the HDS : the possibility of substitution of the leaf spring`s material from Inconel 718 to Zircaloy and the effects on the HDS`s elastic characteristics according to the variation of leaf thickness and the number of leaves composing the HDS. (author). 34 refs., 33 tabs., 37 figs.

  12. Metabolic interaction between anthocyanin and lignin biosynthesis is associated with peroxidase FaPRX27 in strawberry fruit.

    Science.gov (United States)

    Ring, Ludwig; Yeh, Su-Ying; Hücherig, Stephanie; Hoffmann, Thomas; Blanco-Portales, Rosario; Fouche, Mathieu; Villatoro, Carmen; Denoyes, Béatrice; Monfort, Amparo; Caballero, José Luis; Muñoz-Blanco, Juan; Gershenson, Jonathan; Schwab, Wilfried

    2013-09-01

    Plant phenolics have drawn increasing attention due to their potential nutritional benefits. Although the basic reactions of the phenolics biosynthetic pathways in plants have been intensively analyzed, the regulation of their accumulation and flux through the pathway is not that well established. The aim of this study was to use a strawberry (Fragaria × ananassa) microarray to investigate gene expression patterns associated with the accumulation of phenylpropanoids, flavonoids, and anthocyanins in strawberry fruit. An examination of the transcriptome, coupled with metabolite profiling data from different commercial varieties, was undertaken to identify genes whose expression correlated with altered phenolics composition. Seventeen comparative microarray analyses revealed 15 genes that were differentially (more than 200-fold) expressed in phenolics-rich versus phenolics-poor varieties. The results were validated by heterologous expression of the peroxidase FaPRX27 gene, which showed the highest altered expression level (more than 900-fold). The encoded protein was functionally characterized and is assumed to be involved in lignin formation during strawberry fruit ripening. Quantitative trait locus analysis indicated that the genomic region of FaPRX27 is associated with the fruit color trait. Down-regulation of the CHALCONE SYNTHASE gene and concomitant induction of FaPRX27 expression diverted the flux from anthocyanins to lignin. The results highlight the competition of the different phenolics pathways for their common precursors. The list of the 15 candidates provides new genes that are likely to impact polyphenol accumulation in strawberry fruit and could be used to develop molecular markers to select phenolics-rich germplasm.

  13. SIFT-MS and FA-MS methods for ambient gas phase analysis: developments and applications in the UK.

    Science.gov (United States)

    Smith, David; Španěl, Patrik

    2015-04-21

    Selected ion flow tube mass spectrometry, SIFT-MS, a relatively new gas/vapour phase analytical method, is derived from the much earlier selected ion flow tube, SIFT, used for the study of gas phase ion-molecule reactions. Both the SIFT and SIFT-MS techniques were conceived and developed in the UK, the former at Birmingham University, the latter at Keele University along with the complementary flowing afterglow mass spectrometry, FA-MS, technique. The focus of this short review is largely to describe the origins, developments and, most importantly, the unique features of SIFT-MS as an analytical tool for ambient analysis and to indicate its growing use to analyse humid air, especially exhaled breath, its unique place as a on-line, real time analytical method and its growing use and applications as a non-invasive diagnostic in clinical diagnosis and therapeutic monitoring, principally within several UK universities and hospitals, and briefly in the wider world. A few case studies are outlined that show the potential of SIFT-MS and FA-MS in the detection and quantification of metabolites in exhaled breath as a step towards recognising pathophysiology indicative of disease and the presence of bacterial and fungal infection of the airways and lungs. Particular cases include the detection of Pseudomonas aeruginosa infection of the airways of patients with cystic fibrosis (SIFT-MS) and the measurement of total body water in patients with chronic kidney disease (FA-MS). The growing exploitation of SIFT-MS in other areas of research and commerce are briefly listed to show the wide utility of this unique UK-developed analytical method, and future prospects and developments are alluded to.

  14. Natural stone in ventilated façades: method for determining the appropriate moisture content for strength tests

    Directory of Open Access Journals (Sweden)

    Vielba, C.

    2009-03-01

    Full Text Available The article discusses the effect of the assumptions about moisture content on the design of ventilated façades with porous natural stone cladding. Moisture is shown to impact cladding strength and condition its dimensions. For this reason the performance of such façades when exposed to water should be analyzed more fully than specified in EN standards. An example is given of how to conduct such an analysis, along with guidelines for choosing a moisture content value that correctly reflects the absorption levels attainable in such façades. This value is then used to determine panel mechanical strength. Application of the method to three sandstones from Northeastern Spain showed the utility of the type of analysis proposed.El artículo muestra la influencia que tiene la consideración del contenido de humedad en el diseño de las fachadas trasventiladas hechas de piedra natural porosa. Se comprueba cómo este factor influye en la resistencia de las placas y condiciona sus dimensiones. Por ello se propone realizar un análisis más amplio del comportamiento frente al agua, que el propuesto por las normas EN. A través de un ejemplo, se muestra cómo realizar dicho análisis y se dan pautas para elegir un contenido de humedad que represente el grado de absorción que la piedra pueda alcanzar en este tipo de fachadas. Con este contenido, se determinarán las resistencias mecánicas de las placas. El estudio se ha aplicado a tres areniscas de la misma zona del noreste español, obteniendo resultados que demuestran la utilidad del análisis que se propone.

  15. Long-term phosphodiesterase 5 inhibitor administration reduces inflammatory markers and heat-shock proteins in cavernous tissue of Zucker diabetic fatty rat (ZDF/fa/fa).

    Science.gov (United States)

    Toblli, J E; Cao, G; Angerosa, M; Rivero, M

    2015-01-01

    Oxidative stress and nitrosative stress present in type 2 diabetes mellitus (T2DM) and metabolic syndrome (MS) induce inflammatory response in diverse tissues including cavernous tissue (CT). Heat-shock proteins (HSPs) have an important role in modulating and repairing tissue injury, although their participation in CT in T2DM is unclear. Beyond the specific action of phosphodiesterase type 5 inhibitors (PDE5i) on erectile function, it has been proposed that chronic administration of these agents improves endothelial function and ameliorates fibrotic changes. The aim of this study was to determine in CT of Zucker Diabetic Fatty (ZDF) rat, an experimental model of T2DM and MS: (1) the degree of oxidative stress and nitrosative stress; (2) the magnitude of inflammatory markers such as tumor necrosis factor-α (TNFα) and interleukin 6 (IL6); (3) immunoexpression of HSP70 and HSP27; (4) how a long-term PDE5i administration may modify these variables. For 6 months, (1) untreated ZDF; (2) ZDF+Sildenafil (Sil) and (3) control Lean Zucker Rat (LZR) received no treatment, were studied. Penises were processed for functional 'in vitro' studies, oxidative and nitrosative stress evaluation and immunohistochemistry in CT using TNFα; IL6; nitrotyrosine, HSP70 and HSP27 antibodies. ZDF+Sil presented better relaxation in corporal strips versus untreated ZDF. Furthermore, ZDF+Sil presented less lipoperoxidation in CT versus untreated ZDF. The activity of antioxidant enzymes CuZn superoxide dismutase (CuZnSOD) and glutathione peroxidase (GPx) was also reduced in untreated ZDF in CT along with a decrease in glutathione versus untreated ZDF. Nitrotyrosine expression was increased in untreated-ZDF rats versus ZDF+Sil and LZR. TNFα and IL6 were decreased in CT in ZDF+Sil versus untreated ZDF. Additionally, the expression of HSP70 and HSP27 was reduced in CT in ZDF+Sil versus untreated ZDF. In conclusion, this study provides substantial evidence supporting a protective role of a long

  16. 宾得发布D FA645 MACRO 90mm F2.8ED AW SR镜头

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    宾得发布D FA645 MACRO 90mm F2.8 ED AW SR中画幅镜头。该镜头装在645D机身上等效35mm焦距为71mm,采用9组11片结构,最近对焦距离0.41m。9片光圈叶片,外观尺寸91mm×117mm,重1040g。

  17. Representing geometry shape and matter of a complex façade: the church of San Placido in Catania

    Directory of Open Access Journals (Sweden)

    Mariateresa Galizia

    2012-11-01

    Full Text Available This study investigates the potentialities, the limits and the advantages of the 3D model obtained by means of 3D laser scanner for the documentation and the geometric, formal, materic stdudy of an historical complex façade such as the one of the eight-century church of san Placido in Catania. The aim is also to detect possible protocols useful for 3D modeling and data management. The carried out elaborations have absorbed the research team in the exploration of the subsequent 3D digital models which represent, each one with its own feature, the geometry and the measure, the shape and the matter.

  18. Regulation of lipid synthesis genes and milk fat production in human mammary epithelial cells during secretory activation.

    Science.gov (United States)

    Mohammad, Mahmoud A; Haymond, Morey W

    2013-09-15

    Expression of genes for lipid biosynthetic enzymes during initiation of lactation in humans is unknown. Our goal was to study mRNA expression of lipid metabolic enzymes in human mammary epithelial cell (MEC) in conjunction with the measurement of milk fatty acid (FA) composition during secretory activation. Gene expression from mRNA isolated from milk fat globule (MFG) and milk FA composition were measured from 6 h to 42 days postpartum in seven normal women. Over the first 96 h postpartum, daily milk fat output increased severalfold and mirrored expression of genes for all aspects of lipid metabolism and milk FA production, including lipolysis at the MEC membrane, FA uptake from blood, intracellular FA transport, de novo FA synthesis, FA and glycerol activation, FA elongation, FA desaturation, triglyceride synthesis, cholesterol synthesis, and lipid droplet formation. Expression of the gene for a key lipid synthesis regulator, sterol regulatory element-binding transcription factor 1 (SREBF1), increased 2.0-fold by 36 h and remained elevated over the study duration. Expression of genes for estrogen receptor 1, thyroid hormone-responsive protein, and insulin-induced 2 increased progressively to plateau by 96 h. In contrast, mRNA of peroxisome proliferator-activated receptor-γ decreased severalfold. With onset of lactation, increased de novo synthesis of FA was the most prominent change in milk FA composition and mirrored the expression of FA synthesis genes. In conclusion, milk lipid synthesis and secretion in humans is a complex process requiring the orchestration of a wide variety of pathways of which SREBF1 may play a primary role.

  19. Design of an effective bifunctional catalyst organotriphosphonic acid-functionalized ferric alginate (ATMP-FA) and optimization by Box-Behnken model for biodiesel esterification synthesis of oleic acid over ATMP-FA.

    Science.gov (United States)

    Liu, Wei; Yin, Ping; Liu, Xiguang; Qu, Rongjun

    2014-12-01

    Biodiesel production has become an intense research area because of rapidly depleting energy reserves and increasing petroleum prices together with environmental concerns. This paper focused on the optimization of the catalytic performance in the esterification reaction of oleic acid for biodiesel production over the bifunctional catalyst organotriphosphonic acid-functionalized ferric alginate ATMP-FA. The reaction parameters including catalyst amount, ethanol to oleic acid molar ratio and reaction temperature have been optimized by response surface methodology (RSM) using the Box-Behnken model. It was found that the reaction temperature was the most significant factor, and the best conversion ratio of oleic acid could reach 93.17% under the reaction conditions with 9.53% of catalyst amount and 8.62:1 of ethanol to oleic acid molar ratio at 91.0 °C. The research results show that two catalytic species could work cooperatively to promote the esterification reaction, and the bifunctional ATMP-FA is a potential catalyst for biodiesel production.

  20. Glucomannan or Glucomannan Plus Spirulina-Enriched Squid-Surimi Diets Reduce Histological Damage to Liver and Heart in Zucker fa/fa Rats Fed a Cholesterol-Enriched and Non-Cholesterol-Enriched Atherogenic Diet.

    Science.gov (United States)

    Vázquez-Velasco, Miguel; González-Torres, Laura; García-Fernández, Rosa A; Méndez, María Teresa; Bastida, Sara; Benedí, Juana; González-Muñoz, María José; Sánchez-Muniz, Francisco J

    2017-06-01

    Glucomannan-enriched squid surimi improves cholesterolemia and liver antioxidant status. The effect of squid surimi enriched with glucomannan or glucomannan plus spirulina on liver and heart structures and cell damage markers was tested in fa/fa rats fed highly saturated-hyper-energetic diets. Animals were fed 70% AIN-93M rodent diet plus six versions of 30% squid surimi for 7 weeks: control (C), glucomannan (G), and glucomannan plus spirulina (GS). The cholesterol-control (HC), cholesterol-glucomannan (HG), and cholesterol-glucomannan plus spirulina (HGS) groups were given similar diets that were enriched with 2% cholesterol and 0.4% cholic acid. G and GS diets versus C diet significantly inhibited weight gain and lowered plasma alanine aminotransferase and aspartate aminotransferase, liver steatosis, lipogranulomas, and total inflammation and alteration scores. The hypercholesterolemic agent significantly increased the harmful effects of the C diet. Liver weight, the hepatosomatic index, all damage markers, and total histological scoring rose for HC versus C (at least P spirulina were observed except for the total liver alteration score. In conclusion, glucomannan and glucomannan plus spirulina blocked the highly saturated-hyper-energetic diet negative effects both with and without added cholesterol. Results suggest the usefulness of including these functional ingredients in fish products.

  1. GLUCOMANNAN AND GLUCOMANNAN PLUS SPIRULINA-ENRICHED SQUID-SURIMI ADDED TO HIGH SATURATED DIET AFFECT GLYCEMIA, PLASMA AND ADIPOSE LEPTIN AND ADIPONECTIN LEVELS IN GROWING FA/FA RATS.

    Science.gov (United States)

    Vázquez-Velasco, Miguel; González-Torres, Laura; Méndez, María Teresa; Bastida, Sara; Benedí, Juana; González-Muñoz, M José; Sánchez-Muniz, Francisco J

    2015-12-01

    Type 2 diabetes is a very prevalent chronic disease. Among dietary factors for its prevention and treatment, interest has grown in satiating fibre (konjac glucomannan) and spirulina. Our previous studies suggest that glucomannan itself and/or in conjunction to spirulina displayed hypolipemic and antioxidant effects when incorporated to squid surimi as functional ingredients. The present study aims to determine whether glucomannan- enriched or glucomannan plus spirulina-enriched squid-surimi improve plasma glucose and insulin levels in Zucker fa/fa rats fed a high saturated fat diet. Twenty four growing rats, divided into three groups, were given modified AIN-93M diets for seven weeks: 30% squid-surimi control diet (C), 30% glucomannan-enriched squid-surimi diet (G) and 30% glucomannan plus spirulina-enriched squid-surimi diet (GS). All rats became hyperglycemics and hyperinsulinemics, but G and GS diets induced significantly lower glucose levels (20%; p Spirulina inclusion increased insulin availability. Although results are promising, the utility of consuming glucomannan surimis as part of usual diets demands future studies. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  2. Microbial deterioration of artistic tiles from the façade of the Grande Albergo Ausonia & Hungaria (Venice, Italy).

    Science.gov (United States)

    Giacomucci, Lucia; Bertoncello, Renzo; Salvadori, Ornella; Martini, Ilaria; Favaro, Monica; Villa, Federica; Sorlini, Claudia; Cappitelli, Francesca

    2011-08-01

    The Grande Albergo Ausonia & Hungaria (Venice Lido, Italy) has an Art Nouveau polychrome ceramic coating on its façade, which was restored in 2007. Soon after the conservation treatment, many tiles of the façade decoration showed coloured alterations putatively attributed to the presence of microbial communities. To confirm the presence of the biological deposit and the stratigraphy of the Hungaria tiles, stereomicroscope, optical and environmental scanning electron microscope observations were made. The characterisation of the microbial community was performed using a PCR-DGGE approach. This study reported the first use of a culture-independent approach to identify the total community present in biodeteriorated artistic tiles. The case study examined here reveals that the coloured alterations on the tiles were mainly due to the presence of cryptoendolithic cyanobacteria. In addition, we proved that the microflora present on the tiles was generally greatly influenced by the environment of the Hungaria hotel. We found several microorganisms related to the alkaline environment, which is in the range of the tile pH, and related to the aquatic environment, the presence of the acrylic resin Paraloid B72® used during the 2007 treatment and the pollutants of the Venice lagoon.

  3. Stathmin1 overexpression in hypopharyngeal squamous cell carcinoma: A new promoter in FaDu cell proliferation and migration

    Science.gov (United States)

    Chen, Yan; Zhang, Qicheng; Ding, Chuanjin; Zhang, Xiaobo; Qiu, Xiaoxia; Zhang, Zhenxin

    2017-01-01

    Stathmin1, a microtubule-destabilizing phosphoprotein, is considered to play a crucial role in regulating cellular microtubule dynamics and controlling mitosis. Previous studies have showed that STMN1 is highly expressed in many human malignancies and is related to development, invasion and metastasis of tumors. However, its expression pattern, clinical performance and functional roles in hypopharyngeal squamous cell carcinoma (HSCC) have not been addressed. In this study, we found that STMN1 was significantly elevated in HSCC and its expression level was correlated with poor differentiation (P<0.001), clinical stage (P<0.001), large tumor size (P=0.001) and lymph node metastasis (P=0.008). A positive correlation between STMN1 and Ki-67 expression was also exhibited. High STMN1 expression predicted poor survival. Furthermore, we found that knockdown of STMN1 by siRNAs inhibited the FaDu cell proliferation and migration. Moreover, decreased STMN1 expression in FaDu cells reversed the acquisition of EMT phenotype by upregulating E-cadherin, as well as reduced vimentin expression at protein and mRNA levels. These results suggested that STMN1 plays an important role in proliferation and migration of HSCC and may be used as a potential prognostic biomarker or therapeutic target of HSCC. PMID:27878293

  4. The mechanisms underlying overgeneral autobiographical memory: an evaluative review of evidence for the CaR-FA-X model.

    Science.gov (United States)

    Sumner, Jennifer A

    2012-02-01

    Overgeneral autobiographical memory (OGM) has been found to be an important cognitive phenomenon with respect to depression and trauma-related psychopathology (e.g., posttraumatic stress disorder), and researchers have been interested in better understanding the factors that contribute to this proposed vulnerability factor. The most prominent model of mechanisms underlying OGM to date is Williams et al.'s (2007) CaR-FA-X model. This model proposes that three processes influence OGM: capture and rumination, functional avoidance, and impaired executive control. The author reviews the current state of support for the CaR-FA-X model by evaluating 38 studies that have examined OGM and one or more mechanisms of the model. Collectively, these studies reveal robust support for associations between OGM and both rumination and impaired executive control. OGM also appears to be a cognitive avoidance strategy, and there is evidence that avoiding the retrieval of specific memories reduces distress after an aversive event, at least in the short term. Important issues that have been left unresolved are highlighted, including the nature of the capture phenomenon, the role of trauma in functional avoidance, and the developmental nature of functional avoidance. Recommendations for future research that will enhance understanding of the factors that contribute to OGM are suggested.

  5. Genetic modifiers of Lepr{sup fa} associated with variability in insulin production and susceptibility to NIDDM

    Energy Technology Data Exchange (ETDEWEB)

    Chung, W.K.; Zheng, M.; Chua, M. [Rockefeller Univ., New York, NY (United States)] [and others

    1997-05-01

    In an attempt to identify the genetic basis for susceptibility to non-insulin-dependent diabetes mellitus within the context of obesity, we generated 401 genetically obese Lepr{sup fa}/Lepr{sup fa} F2 WKY13M intercross rats that demonstrated wide variation in multiple phenotypic measures related to diabetes, including plasma glucose concentration, percentage of glycosylated hemoglobin, plasma insulin concentration, and pancreatic islet morphology. Using selective genotyping genome scanning approaches, we have identified three quantitative trait loci (QTLs) on Chr. 1 (LOD 7.1 for pancreatic morpholology), Chr. 12 (LOD 5.1 for body mass index and LOD 3.4 for plasma glucose concentration), and Chr. 16 (P < 0.001 for genotype effect on plasma glucose concentration). The obese F2 progeny demonstrated sexual dimorphism for these traits, with increased diabetes susceptibility in the males appearing at approximately 6 weeks of age, as sexual maturation occurred. For each of the QTLs, the linked phenotypes demonstrated sexual dimorphism (more severe affection in males). The QTL on Chr. 1 maps to a region vicinal to that previously linked to adiposity in studies of diabetes susceptibility in the nonobese Goto-Kakizaki rat, which is genetically closely related to the Wistar counterstrain we employed. Several candidate genes, including tubby (tub), multigenic obesity 1 (Mob1), adult obesity and diabetes (Ad), and insulin-like growth factor-2 (Igf2), map to murine regions homologous to the QTL region identified on rat Chr. 1. 60 refs., 5 figs., 4 tabs.

  6. C19orf12 and FA2H mutations are rare in Italian patients with neurodegeneration with brain iron accumulation.

    Science.gov (United States)

    Panteghini, Celeste; Zorzi, Giovanna; Venco, Paola; Dusi, Sabrina; Reale, Chiara; Brunetti, Dario; Chiapparini, Luisa; Zibordi, Federica; Siegel, Birgit; Siegel, Brigitte; Garavaglia, Barbara; Simonati, Alessandro; Bertini, Enrico; Nardocci, Nardo; Tiranti, Valeria

    2012-06-01

    Neurodegeneration with brain iron accumulation (NBIA) defines a wide spectrum of clinical entities characterized by iron accumulation in specific regions of the brain, predominantly in the basal ganglia. We evaluated the presence of FA2H and C19orf12 mutations in a cohort of 46 Italian patients with early onset NBIA, which were negative for mutations in the PANK2 and PLA2G6 genes. Follow-up molecular genetic and in vitro analyses were then performed. We did not find any mutations in the FA2H gene, although we identified 3 patients carrying novel mutations in the C19orf12 gene. The recent discovery of new genes responsible for NBIA extends the spectrum of the genetic investigation now available for these disorders and makes it possible to delineate a clearer clinical-genetic classification of different forms of this syndrome. A large fraction of patients still remain without a molecular genetics diagnosis, suggesting that additional NBIA genes are still to be discovered.

  7. FA safety testing of HTR fuel pebbles irradiated in the High Flux Reactor in Petten

    Energy Technology Data Exchange (ETDEWEB)

    Seeger, O., E-mail: oliver.seeger@rwth-aachen.de [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Laurie, M., E-mail: mathias.laurie@ec.europa.eu [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Abjani, A. El; Ejton, J.; Boudaud, D.; Freis, D.; Carbol, P.; Rondinella, V.V. [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Fütterer, M. [European Commission, Joint Research Centre (JRC), Institute for Energy and Transport (IET), Nuclear Reactor Integrity Assessment and Knowledge Management Unit, PO Box 2, 1755 ZG Petten (Netherlands); Allelein, H.-J. [Lehrstuhl für Reaktorsicherheit und -technik an der RWTH Aachen, Kackertstraße 9, 52072 Aachen (Germany)

    2016-09-15

    The Cold Finger Apparatus (KühlFinger-Apparatur—KüFA) in operation at JRC-ITU is designed to experimentally scrutinize the effects of Depressurization LOss of Forced Circulation (D-LOFC) accident scenarios on irradiated High Temperature Reactor (HTR) fuel pebbles. Up to 1600 °C, the reference maximum temperature for these accidents, high-quality German HTR fuel pebbles have already demonstrated a small fission product release. This paper discusses and compares the releases obtained from KüFA-testing the pebbles HFR-K5/3 and HFR-EU1/3, which were both irradiated in the High Flux Reactor (HFR) in Petten. We present the time-dependent fractional release of the volatile fission product {sup 137}Cs as well as the fission gas {sup 85}Kr for both pebbles. For HFR-EU1/3 the isotopes {sup 134}Cs and {sup 154}Eu as well as the shorter-lived {sup 110m}Ag have also been measured. A detailed description of the experimental setup and its accuracy is given. The data for the recently tested pebbles is discussed in the context of previous results.

  8. What can the Samoan "Fa'afafine" teach us about the Western concept of gender identity disorder in childhood?

    Science.gov (United States)

    Vasey, Paul L; Bartlett, Nancy H

    2007-01-01

    This article examines whether gender identity disorder in childhood (GIDC) constitutes a mental disorder as outlined by the Diagnostic and Statistical Manual of Mental Disorders, 4th edition (DSM-IV-TR). Data were collected in Samoa, a culture that is characterized by a high degree of social tolerance towards feminine males who are known locally as fa'afafine. The study location was chosen because, unlike Western locales, it afforded the opportunity to examine whether gender-atypical behavior, gender-atypical identity, and sex-atypical identity, in and of themselves, cause distress in sex/gender variant individuals, while simultaneously controlling for the confounding effects of extreme societal intolerance towards such individuals. Because of our focus on the DSM-IV-TR's diagnosis of GIDC, we were specifically interested in ascertaining whether adult fa'afafine recalled a strong and persistent cross-gender identification in childhood, a sense of inappropriateness in the male-typical gender role, a discomfort with their sex, or distress associated with any of the above. In addition, we sought to determine whether parental encouragement or discouragement of cross-gender behaviors influence feelings of distress in relation to the behaviors in question. Based on the cross-cultural information presented here, we conclude that the diagnostic category of GIDC should not occur in its current form in future editions of the DSM, as there is no compelling evidence that cross-gender behaviors or identities, in and of themselves, cause distress in the individual.

  9. C-axis electrical resistivity of PrO1-aFaBiS2 single crystals

    Science.gov (United States)

    Nagao, Masanori; Miura, Akira; Watauchi, Satoshi; Takano, Yoshihiko; Tanaka, Isao

    2015-08-01

    The high anisotropy in RO1-aFaBiS2 (R denotes a rare-earth element) superconductors demonstrates their potential use as intrinsic Josephson junctions, considering the weak coupling among BiS2-PrO(F)-BiS2 (superconducting-normal-superconducting) layers along the c-axis. We grew PrO1-aFaBiS2 single crystals using CsCl/KCl flux. The superconducting anisotropies of the grown single crystals were estimated to be approximately 40-50 from the effective mass model. The c-axis transport properties were characterized using single-crystal s-shaped intrinsic Josephson junctions with a focused ion beam. Along the c-axis, the crystals showed zero resistivity at 2.7 K and a critical current density of 1.33 × 103 A/cm2 at 2.0 K. The current-voltage curve along the c-axis displayed hysteresis. The c-axis transport measurements under a magnetic field parallel to the ab-plane revealed a “lock-in” state due to the Josephson vortex flow, indicating that BiS2 superconductors are promising candidates for intrinsic Josephson junctions.

  10. The mechanisms underlying overgeneral autobiographical memory: An evaluative review of evidence for the CaR-FA-X model

    Science.gov (United States)

    Sumner, Jennifer A.

    2011-01-01

    Overgeneral autobiographical memory (OGM) has been found to be an important cognitive phenomenon with respect to depression and trauma-related psychopathology (e.g., posttraumatic stress disorder), and researchers have been interested in better understanding the factors that contribute to this proposed vulnerability factor. The most prominent model of mechanisms underlying OGM to date is Williams et al.’s (2007) CaR-FA-X model. This model proposes that three processes influence OGM: capture and rumination, functional avoidance, and impaired executive control. The author reviews the current state of support for the CaR-FA-X model by evaluating 38 studies that have examined OGM and one or more mechanisms of the model. Collectively, these studies reveal robust support for associations between OGM and both rumination and impaired executive control. OGM also appears to be a cognitive avoidance strategy, and there is evidence that avoiding the retrieval of specific memories reduces distress after an aversive event, at least in the short term. Important issues that have been left unresolved are highlighted, including the nature of the capture phenomenon, the role of trauma in functional avoidance, and the developmental nature of functional avoidance. Recommendations for future research that will enhance understanding of the factors that contribute to OGM are suggested. PMID:22142837

  11. Composition of Façon de Venise glass from early 17th century London in comparison with luxury glass of the same age

    Science.gov (United States)

    Cagno, S.; De Raedt, I.; Jeffries, T.; Janssens, K.

    SEM-EDX and LA-ICP-MS analyses were performed on a set of early 17th century London glass fragments. The samples originate from two archaeological sites (Aldgate and Old Broad Street) where glass workshops were active in this period. The great majority of the samples are made of soda glass. Two distinct compositional groups are observed, each typical of one site of provenance. The samples originating from the Old Broad Street excavation feature a silica-soda-lime composition, with a moderate amount of potash. The samples from Aldgate are richer in potassium and feature higher amounts of trace elements such as Rb, Zr and Cu. The distinction between the two groups stems from different flux and silica sources used for glassmaking. A comparison with different European glass compositions of that time reveals no resemblance with genuine Venetian production, yet the composition of the Old Broad Street glass shows a close similarity to that of fragments produced `à la façon de Venise' in Antwerp at the end of the 16th century. This coincides with historical sources attesting the arrival of glassworkers from the Low Countries in England and suggests that a transfer of technology took place near the turn of the century.

  12. Assateague Island sediment core radiochemistry data from March-April 2014, USGS Field Activity Number 2014-301-FA

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The influence of tropical and extratropical cyclones on coastal wetlands and marshes is highly variable in both space and time and depends on a number of climatic,...

  13. Assateague Island sediment core radiochemistry data from March-April 2014, USGS Field Activity Number 2014-301-FA

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The influence of tropical and extratropical cyclones on coastal wetlands and marshes is highly variable in both space and time and depends on a number of climatic,...

  14. FA/OⅡ型螯合剂对多层Cu布线CMP后BTA去除的研究∗%Study of FA/OⅡ chelating agent on BTA removal in post-CMP cleaning

    Institute of Scientific and Technical Information of China (English)

    邓海文; 檀柏梅; 张燕; 高宝红; 王辰伟; 顾张冰

    2016-01-01

    在化学机械抛光(CMP)过程中,加入苯并三氮唑(BTA)抑制 Cu 界面和布线条的腐蚀.但同时,会与Cu发生化学反应生成的Cu-BTA钝化膜是CMP后主要的清洗对象之一.采用FA/OⅡ型螯合剂作为清洗液的主要成分,采用接触角测试仪及原子力显微镜来表征BTA的去除效果.通过改变 FA/OⅡ型螯合剂的浓度完成一系列对比实验,确定最佳的清洗效果.通过对比实验得知,当清洗液中螯合剂的浓度为1.50×10-4~200×10-4时,此时清洗液的pH 值>10,能有效去除Cu-BTA 钝化膜以及其它残留的有机物,接触角下降到29°,表面的粗糙度较低.%In the chemical mechanical polishing (CMP)process,benzotriazole (BTA)was used as corrosion in-hibitor in the copper CMP.The insoluble Cu-BTA passivation film was one of the main targets in post-CMP cleaning.FA/OII chelating agent was the main component of the cleaning liquid,which was used in this paper. Contact angle measurements and atomic force microscope was used to monitor the removal of BTA.By varying the concentration of chelating agent,a series of experiments were performed to determine the best cleaning re-sults.When the concentration of chelating agent between 1.50×10-4 and 2.00×10-4 ,the pH value was higher than 10,can effectively remove the Cu-BTA passivation film and other organic residue and the contact angle de-creased to 29°.The surface roughness was better.The proposed cleaning solution showed good ability in remo-ving BTA from the copper surface.

  15. Activation of Rejected Fly Ash Using Flue Gas Desulphurization (FGD) Sludge

    Institute of Scientific and Technical Information of China (English)

    QIAO Xiu-chen; POON Chisun; LIN Zong-shou

    2003-01-01

    Low-grade fly ash ( rejected fly ash, rFA ), a significant portion of the pulverized fuel ash ( PFA ) produced from coal-fired power plauts and rejected from the ash classifying process, remains unused due to its high carbon content and large particle size ( > 45μm ). But it is thonght that the rejected ash may have potential uses in chemical stabilization/solidification ( S/S ) processes which need relatively lower strengths and a lower chemical reactivity. Flue Gas Desulphurisation ( FGD ) sludge is a by-product of air pollution control equipment in coal fired power plants whose chemical composition is mainly gypsum. As there is no effective usage of both of these two ntaterials , it is of interest to research on the possible octtivation of rFA using FGD . This paper presents experimental results of a study on the properties of rFA activated by the FGD in rFA-cement pastes. Different percetages of FGD were added into the mix to study the effects of the FGD on the reaction of the rFA blended cement pastes.The results show that FGD takes effect as an activator only at late curing ages. Adding Ca ( OH)2 enhances the effect of FGD on activating the hytration of rFA. Also, 10% FGD by weight of rFA is the optimal addition in the rFA-cement pastes. The results of the compressive strength measurements correlate well with the porosity results.

  16. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction.

    Science.gov (United States)

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de Los Santos, Berta; Arroyo, Francisco T; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.

  17. Partial activation of SA- and JA-defensive pathways in strawberry upon Colletotrichum acutatum interaction

    Directory of Open Access Journals (Sweden)

    FRANCISCO AMIL-RUIZ

    2016-07-01

    Full Text Available Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5 and FaPR10 were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.

  18. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

    Science.gov (United States)

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de los Santos, Berta; Arroyo, Francisco T.; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L.

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  19. Growth, physicochemical properties, and morphogenesis of Chinese wild-type PRV Fa and its gene-deleted mutant strain PRV SA215

    Directory of Open Access Journals (Sweden)

    Tang Shanhu

    2011-06-01

    Full Text Available Abstract Background PRV Fa is common in China and causes most of the pseudorabies in the pig industry. A PRV SA215 strain with deleted gE, gI, and TK genes was constructed to develop a commercial attenuated live vaccine. However, the physicochemical properties, growth pattern, penetration kinetics, and morphogenesis of the PRV SA215 and its parental PRV Fa strain are unclear. Results A series of experiments were conducted to characterize both strains and provide more information. PRV Fa and PRV SA215 were found to have similar penetration patterns, with about 5 min half-time of penetration. The SA215 strain exhibited a slight delay in entry compared with PRV Fa. In the one-step growth test, the titers of the SA215 strain were first detected at 8 h, rapidly increased, and peaked at 12 h. A plateau was formed between 12-36 h of culturing. PRV SA215 showed delayed replication and approximately 10-30-fold lower titers during 0-16 h of culturing compared with the PRV-Fa strain. After 16 h, the PRV Fa titers dramatically decreased, whereas those of PRV SA215 were prolonged to 36 h and reached a titer value equal to that of PRV Fa and then decreased. Both strains were sensitive to both heat and acid-alkali treatments; however, PRV Fa was relatively more stable to heat treatment than PRV SA215. Both strains could propagate in the cultures with pH values from 5.0 to 9.0. Cultures with pH below 3.0 or above 11.0 were fatal to both strains. Both strains had considerable resistance to freeze-thawing treatments. Morphogenetic investigations showed that typical phases in the maturation pathway were observed in the PRV Fa-infected PK15 cells, whereas secondary envelopment was not observed in the PRV SA215 strain. Instead, capsid aggregations with concomitants of electrodense materials were observed. Conclusions These results suggest that PRV SA215 is a promising strain for vaccine development

  20. Demethylation of oligogalacturonides by FaPE1 in the fruits of the wild strawberry Fragaria vesca triggers metabolic and transcriptional changes associated with defence and development of the fruit.

    Science.gov (United States)

    Osorio, Sonia; Bombarely, Aureliano; Giavalisco, Patrick; Usadel, Björn; Stephens, Camilla; Aragüez, Irene; Medina-Escobar, Nieves; Botella, Miguel A; Fernie, Alisdair R; Valpuesta, Victoriano

    2011-05-01

    Ectopic expression of the strawberry (Fragaria×ananassa) gene FaPE1 encoding pectin methyl esterase produced in the wild species Fragaria vesca partially demethylated oligogalacturonides (OGAs), which conferred partial resistance of ripe fruits to the fungus Botrytis cinerea. Analyses of metabolic and transcriptional changes in the receptacle of the transgenic fruits revealed channelling of metabolites to aspartate and aromatic amino acids as well as phenolics, flavanones, and sesquiterpenoids, which was in parallel with the increased expression of some genes related to plant defence. The results illustrate the changes associated with resistance to B. cinerea in the transgenic F. vesca. These changes were accompanied by a significant decrease in the auxin content of the receptacle of the ripe fruits of transgenic F. vesca, and enhanced expression of some auxin-repressed genes. The role of these OGAs in fruit development was revealed by the larger size of the ripe fruits in transgenic F. vesca. When taken together these results show that in cultivated F. ananassa FaPE1 participates in the de-esterification of pectins and the generation of partially demethylated OGAs, which might reinforce the plant defence system and play an active role in fruit development.

  1. Similarity Theory Based Radial Turbine Performance and Loss Mechanism Comparison between R245fa and Air for Heavy-Duty Diesel Engine Organic Rankine Cycles

    Directory of Open Access Journals (Sweden)

    Lei Zhang

    2017-01-01

    Full Text Available Organic Rankine Cycles using radial turbines as expanders are considered as one of the most efficient technologies to convert heavy-duty diesel engine waste heat into useful work. Turbine similarity design based on the existing air turbine profiles is time saving. Due to totally different thermodynamic properties between organic fluids and air, its influence on turbine performance and loss mechanisms need to be analyzed. This paper numerically simulated a radial turbine under similar conditions between R245fa and air, and compared the differences of the turbine performance and loss mechanisms. Larger specific heat ratio of air leads to air turbine operating at higher pressure ratios. As R245fa gas constant is only about one-fifth of air gas constant, reduced rotating speeds of R245fa turbine are only 0.4-fold of those of air turbine, and reduced mass flow rates are about twice of those of air turbine. When using R245fa as working fluid, the nozzle shock wave losses decrease but rotor suction surface separation vortex losses increase, and eventually leads that isentropic efficiencies of R245fa turbine in the commonly used velocity ratio range from 0.5 to 0.9 are 3%–4% lower than those of air turbine.

  2. A cyclic and intermittent very low-protein diet can have beneficial effects against advanced diabetic nephropathy in Wistar fatty (fa/fa) rats, an animal model of type 2 diabetes and obesity.

    Science.gov (United States)

    Kitada, Munehiro; Ogura, Yoshio; Suzuki, Taeko; Monnno, Itaru; Kanasaki, Keizo; Watanabe, Ai; Koya, Daisuke

    2017-08-12

    A low-protein diet (LPD), particularly, very low-protein diet (VLPD) is expected for reno-protection in advanced chronic kidney disease, including diabetic nephropathy. We previously also demonstrated that a VLPD clearly improved advanced diabetic nephropathy in a type 2 diabetes and obesity rat. However, clinically, an everyday long-term VLPD contributes to poor adherence, which may be related to controvertial results of an LPD on the suppression for diabetic nephropathy, and has nutritional issues, such as sarcopenia or protein-energy wasting. The aim of this study is to elucidate the reno-protective effect of a cyclic and intermittent VLPD, not an everyday VLPD, against the advanced experimental diabetic nephropathy. Diabetic male Wistar fatty (fa/fa) rats (WFRs) were treated with a standard diet (STD; 23.84% protein) or a cyclic and intermittent VLPD (5.77% protein) consisting of an STD for 3 days and a VLPD for 4 days a week for 20 weeks beginning at 24 weeks of age. A cyclic and intermittent VLPD significantly improved renal hypertrophy, and significantly decreased urinary albumin and liver-type fatty acid binding protein (L-FABP) excretion without changes in body weight or exacerbation of HbA1c levels in diabetic rats. Additionally, diabetes-induced renal injuries including fibrosis, tubular cell damage and inflammation were significantly ameliorated by a cyclic and intermittent VLPD in diabetic rats. Thus, based on our experimental data, a cyclic and intermittent VLPD may be a dietary regimen that is easy to continue and has a less risk of malnutrition, compared to an everyday long-term VLPD, against advanced diabetic nephropathy. This article is protected by copyright. All rights reserved.

  3. FA1 induces pro-inflammatory and anti-adipogenic pathways/markers in human myotubes established from lean, obese and Type 2 diabetic subjects but not insulin resistance

    Directory of Open Access Journals (Sweden)

    Basem M Abdallah

    2013-04-01

    Full Text Available Aims: Delta like 1/FA1 (Dlk1/FA1 is a protein secreted by hormone producing cells in adult human and mice that is known to inhibit adipogenesis. Recent studies demonstrated the role of Dlk1/FA1 in inducing insulin resistance in mice. To investigate the involvement of circulating Dlk1/FA1 in insulin resistance and type 2 diabetes in human subjects, we studied the effects of chronic FA1 on the intermediary metabolism in myotubes established from lean, obese and type 2 diabetic (T2D subjects.Methods: Myotube cultures were established from lean and obese control subjects, and obese T2D subjects and treated with soluble FA1 for 4 days supplemented with/without palmitate (PA. Lipid- and glucose metabolism were studied with labelled precursors while quantitative expression of genes was analyzed using Real-Time PCR.Results: Diabetic myotubes express significantly reduced insulin-stimulated glucose metabolism compared to lean myotubes and a significantly decreased basal PA oxidation. Chronic FA1 exposure did not affect the intermediary metabolism in myotubes. Insulin sensitivity of glucose and lipid metabolism was not affected by chronic FA1 exposure in myotubes established from lean, obese and T2D subjects. Instead, chronic FA1 exposure induced pro-inflammatory cytokines expression (IL6 and CCL2 in association with reducing adipogenic markers (ADD1, AP2, CD36 and PPARg2 in myotubes. Consistent with this observation, addition of FA1 to cultured myotubes was show to significantly inhibit their differentiation into adipocyte. Conclusions: Our results exclude direct effects of FA1 on glucose and lipid metabolism in cultured myotubes established from lean, obese and type 2 diabetic subjects. Therefore, the pathogenesis of FA1-indued IR might mainly be mediated via the FA1-induced stimulation of pro-inflammatory cytokines, which on turn inhibit adipogenesis in human myotubes.

  4. Façade Greening: High-rise apartment building in Milan using pre-stressed concrete slab

    Science.gov (United States)

    Sun, Wenning; Li, Mingxin; Han, Yinong; Wang, Moqi; Ansourian, Peter

    2016-08-01

    In this project, one single level of the Façade Greening was designed and modelled using finite element method in Strand7. A static analysis was performed in order to understand the deflection and the stress due to the extra loads imposed by the soil and plants. The results produced by the linear static solver are compared with the strength of the materials and the European limitations. The maximum tension stress which exceeds the tensile strength in concrete is found in the root of the cantilever balcony. An alternative design of the cantilevered balcony with pre-stressed concrete slab is modelled separately for the balcony. Decrease is found in the tension stress and the significant improvement of deflection of the balcony with pre-stressed concrete slab. The dynamic loads such as wind and earthquake did not suggest significant effect on the pre-stressed concrete slab.

  5. Capability Description for NASA's F/A-18 TN 853 as a Testbed for the Integrated Resilient Aircraft Control Project

    Science.gov (United States)

    Hanson, Curt

    2009-01-01

    The NASA F/A-18 tail number (TN) 853 full-scale Integrated Resilient Aircraft Control (IRAC) testbed has been designed with a full array of capabilities in support of the Aviation Safety Program. Highlights of the system's capabilities include: 1) a quad-redundant research flight control system for safely interfacing controls experiments to the aircraft's control surfaces; 2) a dual-redundant airborne research test system for hosting multi-disciplinary state-of-the-art adaptive control experiments; 3) a robust reversionary configuration for recovery from unusual attitudes and configurations; 4) significant research instrumentation, particularly in the area of static loads; 5) extensive facilities for experiment simulation, data logging, real-time monitoring and post-flight analysis capabilities; and 6) significant growth capability in terms of interfaces and processing power.

  6. A Review on Natural Ventilation-enabling Façade Noise Control Devices for Congested High-Rise Cities

    Directory of Open Access Journals (Sweden)

    Shiu-Keung Tang

    2017-02-01

    Full Text Available This review summarizes the current status of the research and development of natural ventilation-enabling noise control devices for use on the façades of high-rise residential buildings in congested cities. These devices are important for a sustainable urbanized city, as they are supposed to offer good acoustical protection to citizens, allowing for an acceptable level of natural ventilation inside residential units; energy for mechanical ventilation can then be saved. From the information presented in the existing literature, it is concluded that protrusive devices, such as lintels and balconies, are not effective noise screening devices, even if they are installed with sound absorbers and/or reflectors, under the effect of city reverberation. On the contrary, plenum windows and similar structures, which are plenum structures with a staggered air inlet and outlet, are interesting alternatives that are worth rigorous considerations.

  7. Water tunnel flow visualization and wind tunnel data analysis of the F/A-18. [leading edge extension vortex effects

    Science.gov (United States)

    Erickson, G. E.

    1982-01-01

    Six degree of freedom studies were utilized to extract a band of yawing and rolling moment coefficients from the F/A-18 aircraft flight records. These were compared with 0.06 scale model data obtained in a 16T wind tunnel facility. The results, indicate the flight test yawing moment data exhibit an improvement over the wind tunnel data to near neutral stability and a significant reduction in lateral stability (again to anear neutral level). These data are consistent with the flight test results since the motion was characterized by a relatively slo departure. Flight tests repeated the slow yaw departure at M 0.3. Only 0.16 scale model wind tunnel data showed levels of lateral stability similar to the flight test results. Accordingly, geometric modifications were investigated on the 0.16 scale model in the 30x60 foot wind tunnel to improve high angle of attack lateral stability.

  8. Impact of façade window design on energy, daylighting and thermal comfort in nearly zero-energy houses

    DEFF Research Database (Denmark)

    Vanhoutteghem, Lies; Skarning, Gunnlaug Cecilie Jensen; Hviid, Christian Anker;

    2015-01-01

    a solution space defined by targets for daylighting and thermal comfort. In contrast with existing guidelines, the results show an upper limit for energy savings and utilisation of solar gains in south-oriented rooms. Instead, low U-values are needed in both north- and south oriented rooms before large......Appropriate window solutions are decisive for the design of 'nearly zero-energy' buildings with healthy and comfortable indoor environment. This paper focuses on the relationship between size, orientation and glazing properties of façade windows for different side-lit room geometries in Danish...... 'nearly zero-energy' houses. The effect of these parameters on space heating demand, daylighting and thermal environment is evaluated by means of EnergyPlus and DAYSIM and presented in charts illustrating how combinations of design parameters with minimum space heating demand can be selected within...

  9. Monitoring of cycling hypoxia and angiogenesis in FaDu head and neck tumors using a side-firing sensor

    Science.gov (United States)

    Yu, Bing; Shah, Amy; Wang, Bingqing; Rajaram, Narasimhan; Wang, Quanli; Ramanujam, Nirmala; Palmer, Gregory M.; Dewhirst, Mark W.

    2013-03-01

    Many studies have found that hypoxia, particularly cycling hypoxia (CH), can lead to enhanced tumor metastasis and resistance to radiation and chemotherapy. It was also reported that tumor total hemoglobin content (THb), which is directly related to tumor angiogenesis, can have significant impact on tumor's response to radiation and neoadjuvant chemotherapy. There is a growing demand for technologies to measure tumor hypoxia and angiogenesis temporally in vivo. In this paper, a side-firing fiber optic sensor based on a multi-wavelength frequency-domain near infrared spectroscopy (FD-NIRS) instrument was used to quantify tumor oxygenation and hemoglobin concentrations in nude rats bearing human FaDu head and neck (H and N) tumors during normoxia and forced hyperoxia and cyclic hypoxia. Significant increase (with carbogen gas inhalation) or decrease (with reduced O2 supply) in tumor oxygenation was observed. The studies demonstrated the feasibility of the technology for longitudinal monitoring of H and N tumor's response to therapy.

  10. Relationship of lipogenic enzyme activities to the rate of rat liver fatty acid synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Nelson, G.; Kelley, D.; Schmidt, P.; Virk, S.; Serrato, C.

    1986-05-01

    The mechanism by which diet regulates liver lipogenesis is unclear. Here the authors report how dietary alterations effect the activities of key enzymes of fatty acid (FA) synthesis. Male Sprague-Dawley rats, 400-500 g, were fasted for 48h and then refed a fat-free, high carbohydrate (HC) diet (75% cal. from sucrose) for 0,3,9,24 and 48h, or refed a HC diet for 48h, then fed a high-fat (HF) diet (44% cal. from corn oil) for 3,9,24 and 48h. The FA synthesis rate and the activities of acetyl CoA carboxylase (AC), fatty acid synthase (FAS), ATP citrate lyase (CL), and glucose 6-phosphate dehydrogenase (G6PDH) were determined in the livers. FA synthesis was assayed with /sup 3/H/sub 2/O, enzyme activities were measured spectrophotometrically except for AC which was assayed with /sup 14/C-bicarbonate. There was no change in the activity of AC during fasting or on the HC diet. Fasting decreased the rate of FA synthesis by 25% and the activities of FAS and CL by 50%; refeeding the HC diet induced parallel changes in FA synthesis and the activities of FAS, CL, and G6PDH. After 9h on the HF diet, FA synthesis had decreased sharply, AC activity increased significantly while no changes were detected in the other activities. Subsequently FA synthesis did not change while the activities of the enzymes decreased slowly. These enzymes did not appear to regulate FA synthesis during inhibition of lipogenesis, but FAS, CL or G6PDH may be rate limiting in the induction phase. Other key factors may regulate FA synthesis during dietary alterations.

  11. Torroja Institute, the historic building: conservation and characteristics of the materials of its façades

    Directory of Open Access Journals (Sweden)

    Luxán, M. P.

    1999-09-01

    Full Text Available The "Instituto de Ciencias de la Construcción" building is one of the most singular engineering design done by Eduardo Torroja: it was started in 1951. This study focused on the Institute façade-building materials has been carried out in the 100th anniversary of his birth. The analysis has revealed that the design of the original project has been of a great importance for the good conservation of the external walls. The pointing mortar characterization of its brick walls by instrumental techniques (infrared spectroscopy. X-ray diffraction, scanning electron microscopy with EDS reveals that calcite is its main component, with gypsum presence in polluted external zones and silica compounds from the sand. The obtained results indicate a good compatibility between mortars and façade bricks.

    El edificio del "Instituto de Ciencias de la Construcción", construido en 1951, constituye una de las obras singulares del ingeniero Eduardo Torroja. En el centenario de su nacimiento se ha elaborado este estudio, que se centra en los materiales de construcción de las fachadas del Instituto. De su análisis se desprende la importancia del diseño del proyecto original en la buena conservación de los paramentos exteriores. La caracterización de los morteros de junta de las fábricas de ladrillo de los paramentos, mediante técnicas instrumentales (espectroscopia infrarroja, difracción de rayos X, microscopía electrónica de barrido con energías dispersivas de rayos X revela que la calcita es el compuesto principal, con presencia de yeso -especialmente en zonas externas contaminadas- y compuestos silíceos procedentes de la arena. Los resultados sobre las fachadas reflejan una buena compatibilidad entre los morteros de cal y los ladrillos.

  12. Filter paper-assisted cell transfer (FaCT) technique: A novel cell-sampling technique for intraoperative diagnosis of central nervous system tumors.

    Science.gov (United States)

    Kawamura, Jumpei; Kamoshida, Shingo; Shimakata, Takaaki; Hayashi, Yurie; Sakamaki, Kuniko; Denda, Tamami; Kawai, Kenji; Kuwao, Sadahito

    2017-04-01

    Intraoperative diagnosis of central nervous system (CNS) tumors provides critical guidance to surgeons in the determination of surgical resection margins and treatment. The techniques and preparations used for the intraoperative diagnosis of CNS tumors include frozen sectioning and cytologic methods (squash smear and touch imprint). Cytologic specimens, which do not have freezing artifacts, are important as an adjuvant tool to frozen sections. However, if the amount of submitted tissue samples is limited, then it is difficult to prepare both frozen sections and squash smears or touch imprint specimens from a single sample at the same time. Therefore, the objective of this study was to derive cells directly from filter paper on which tumor samples are placed. The authors established the filter paper-assisted cell transfer (FaCT) smear technique, in which tumor cells are transferred onto a glass slide directly from the filter paper sample spot after the biopsy is removed. Cell yields and diagnostic accuracy of the FaCT smears were assessed in 40 CNS tumors. FaCT smears had ample cell numbers and well preserved cell morphology sufficient for cytologic diagnosis, even if the submitted tissues were minimal. The overall diagnostic concordance rates between frozen sections and FaCT smears were 90% and 87.5%, respectively (no significant differences). When combining FaCT smears with frozen sections, the diagnostic concordance rate rose to 92.5%. The current results suggest that the FaCT smear technique is a simple and effective processing method that has significant value for intraoperative diagnosis of CNS tumors. Cancer Cytopathol 2017;125:277-282. © 2016 American Cancer Society. © 2017 American Cancer Society.

  13. CsI Pre-Intercalation in the Inorganic Framework for Efficient and Stable FA1-x Csx PbI3 (Cl) Perovskite Solar Cells.

    Science.gov (United States)

    Zhou, Ning; Shen, Yiheng; Zhang, Yu; Xu, Ziqi; Zheng, Guanhaojie; Li, Liang; Chen, Qi; Zhou, Huanping

    2017-06-01

    Engineering the chemical composition of organic and inorganic hybrid perovskite materials is one of the most feasible methods to boost the efficiency of perovskite solar cells with improved device stability. Among the diverse hybrid perovskite family of ABX3 , formamidinium (FA)-based mixed perovskite (e.g., FA1-x Csx PbI3 ) possesses optimum bandgaps, superior optoelectronic property, as well as thermal- and photostability, which is proven to be the most promising candidate for advanced solar cell. Here, FA0.9 Cs0.1 PbI3 (Cl) is implemented as the light-harvesting layer in planar devices, whereas a low temperature, two-step solution deposition method is employed for the first time in this materials system. This paper comprehensively exploits the role of Cs(+) in the FA0.9 Cs0.1 PbI3 (Cl) perovskite that affects the precursor chemistry, film nucleation and grain growth, and defect property via pre-intercalation of CsI in the inorganic framework. In addition, the resultant FA0.9 Cs0.1 PbI3 (Cl) films are demonstrated to exhibit an improved optoelectronic property with an elevated device power conversion efficiency (PCE) of 18.6%, as well as a stable phase with substantial enhancement in humidity and thermal stability, as compared to that of FAPbI3 (Cl). The present method is able to be further extended to a more complicated (FA,MA,Cs)PbX3 material system by delivering a PCE of 19.8%. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Far-red light photoacclimation (FaRLiP) in Synechococcus sp. PCC 7335. II.Characterization of phycobiliproteins produced during acclimation to far-red light.

    Science.gov (United States)

    Ho, Ming-Yang; Gan, Fei; Shen, Gaozhong; Bryant, Donald A

    2017-02-01

    Phycobilisomes (PBS) are antenna complexes that harvest light for photosystem (PS) I and PS II in cyanobacteria and some algae. A process known as far-red light photoacclimation (FaRLiP) occurs when some cyanobacteria are grown in far-red light (FRL). They synthesize chlorophylls d and f and remodel PS I, PS II, and PBS using subunits paralogous to those produced in white light. The FaRLiP strain, Leptolyngbya sp. JSC-1, replaces hemidiscoidal PBS with pentacylindrical cores, which are produced when cells are grown in red or white light, with PBS with bicylindrical cores when cells are grown in FRL. This study shows that the PBS of another FaRLiP strain, Synechococcus sp. PCC 7335, are not remodeled in cells grown in FRL. Instead, cells grown in FRL produce bicylindrical cores that uniquely contain the paralogous allophycocyanin subunits encoded in the FaRLiP cluster, and these bicylindrical cores coexist with red-light-type PBS with tricylindrical cores. The bicylindrical cores have absorption maxima at 650 and 711 nm and a low-temperature fluorescence emission maximum at 730 nm. They contain ApcE2:ApcF:ApcD3:ApcD2:ApcD5:ApcB2 in the approximate ratio 2:2:4:6:12:22, and a structural model is proposed. Time course experiments showed that bicylindrical cores were detectable about 48 h after cells were transferred from RL to FRL and that synthesis of red-light-type PBS continued throughout a 21-day growth period. When considered in comparison with results for other FaRLiP cyanobacteria, the results here show that acclimation responses to FRL can differ considerably among FaRLiP cyanobacteria.

  15. Identification of differentially expressed genes associated with the enhancement of X-ray susceptibility by RITA in a hypopharyngeal squamous cell carcinoma cell line (FaDu).

    Science.gov (United States)

    Luan, Jinwei; Li, Xianglan; Guo, Rutao; Liu, Shanshan; Luo, Hongyu; You, Qingshan

    2016-06-01

    Next generation sequencing and bio-informatic analyses were conducted to investigate the mechanism of reactivation of p53 and induction of tumor cell apoptosis (RITA)-enhancing X-ray susceptibility in FaDu cells. The cDNA was isolated from FaDu cells treated with 0 X-ray, 8 Gy X-ray, or 8 Gy X-ray + RITA. Then, cDNA libraries were created and sequenced using next generation sequencing, and each assay was repeated twice. Subsequently, differentially expressed genes (DEGs) were identified using Cuffdiff in Cufflinks and their functions were predicted by pathway enrichment analyses. Genes that were constantly up- or down-regulated in 8 Gy X-ray-treated FaDu cells and 8 Gy X-ray + RITA-treated FaDu cells were obtained as RITA genes. Afterward, the protein-protein interaction (PPI) relationships were obtained from the STRING database and a PPI network was constructed using Cytoscape. Furthermore, ClueGO was used for pathway enrichment analysis of genes in the PPI network. Total 2,040 and 297 DEGs were identified in FaDu cells treated with 8 Gy X-ray or 8 Gy X-ray + RITA, respectively. PARP3 and NEIL1 were enriched in base excision repair, and CDK1 was enriched in p53 signaling pathway. RFC2 and EZH2 were identified as RITA genes. In the PPI network, many interaction relationships were identified (e.g., RFC2-CDK1, EZH2-CDK1 and PARP3-EZH2). ClueGO analysis showed that RFC2 and EZH2 were related to cell cycle. RFC2, EZH2, CDK1, PARP3 and NEIL1 may be associated, and together enhance the susceptibility of FaDu cells treated with RITA to the deleterious effects of X-ray.

  16. Evaluating the Possibility of Defining Cut-Off Points for ΔFA% in Order to Differentiate Four Major Types of Peri-Tumoral White Matter Tract Involvement

    Directory of Open Access Journals (Sweden)

    Deilami

    2015-07-01

    Full Text Available Background Diffusion tensor imaging (DTI and its different scalar values such as fractional anisotropy (FA have recently been used for evaluation of peri-tumoral white matter (WM involvement to help define safer surgical excision margins. Objectives The purpose of this study is to evaluate the possibility of defining diagnostic cut-off points for differentiating four major types of peri-tumoral WM involvement using FA. Patients and Methods DTI was performed in 12 patients with high presumption of having brain tumors, on a 1.5 T MRI scanner. DTI data was processed by MedINRIA software. Two-hundred region of interests (ROI were evaluated: 100 in the lesion zone and the rest in the normal WM in the contralateral hemisphere. FA value related to each ROI was measured, and the percentage of FA decrement (ΔFAs% was calculated. Results Of the 100 ROIs on the lesion side, 74 were related to high-grade lesions, 23 to low-grade ones, and three to “gliosis”. There were 54 “infiltrated”, 22 “displaced”, 15 “disrupted”, and 9 “edematous” tracts. The major type of fiber involvement, both in low-grade and high-grade tumors was “infiltrated, whereas “edematous” fibers comprised the minority. ΔFA% was more than -35 for “displaced” and “edematous” fibers, and less than -35 for the majority of “disrupted” ones, but “infiltrated” fibers had scattered distribution. Mean ΔFA% was the least for “disrupted”, followed by “infiltrated”, “edematous” and “displaced” parts. Conclusion Introducing definite diagnostic cut-points was not possible, due to overlap. Based on the fact that “disruption” is the most aggressive process, a sensitivity analysis was carried out for “disrupted” fibers for several presumptive cut-off points.

  17. Biocides from façade coatings in urban surface waters: Estimating the leaching of biocides from render by polyacrylate-water partitioning constants?

    DEFF Research Database (Denmark)

    Bollmann, Ulla E.; Styszko, Kartarzyna; Ou, Yi

    2015-01-01

    Leaching of biocides from façade coatings attracts more and more attention within recent years. In-can as well as film preserving biocides are added to polymer resin based renders and paints in order protect from microbial spoilage. However, several studies revealed that biocides leach from...... the façade coating and are detectable in urban stormwater runoff. The present study focussed on the occurrence of biocides in the aqueous environment - both in urban water close to the sources as well as further away in fresh and marine waters. It could be shown that biocides are ubiquitous in the aqueous...

  18. In-Flight Suppression of a De-Stabilized F/A-18 Structural Mode Using the Space Launch System Adaptive Augmenting Control System

    Science.gov (United States)

    Wall, John; VanZwieten, Tannen; Giiligan Eric; Miller, Chris; Hanson, Curtis; Orr, Jeb

    2015-01-01

    Adaptive Augmenting Control (AAC) has been developed for NASA's Space Launch System (SLS) family of launch vehicles and implemented as a baseline part of its flight control system (FCS). To raise the technical readiness level of the SLS AAC algorithm, the Launch Vehicle Adaptive Control (LVAC) flight test program was conducted in which the SLS FCS prototype software was employed to control the pitch axis of Dryden's specially outfitted F/A-18, the Full Scale Advanced Systems Test Bed (FAST). This presentation focuses on a set of special test cases which demonstrate the successful mitigation of the unstable coupling of an F/A-18 airframe structural mode with the SLS FCS.

  19. The gender-specific role of polymorphisms from the fibrinolytic, renin-angiotensin, and bradykinin systems in determining plasma t-PA and PAI-I levels

    NARCIS (Netherlands)

    Asselbergs, Folkert W.; Williams, Scott M.; Hebert, Patricia R.; Coffey, Christopher S.; Hillege, Hans L.; Navis, Gerjan; Vaughan, Douglas E.; van Gilst, Wiek H.; Moore, Jason H.

    2006-01-01

    Tissue plasminogen activator (t-PA) and plasminogen activator inhibitor I (PAI-I) directly influence thrombus formation and degradation and thus risk for arterial thrombosis. We report here results from a genetic analysis of plasma t-PA and PAI-I levels in a large population-based sample from the PR

  20. 捣法针刺治疗枕神经痛的临床疗效观察%CLINICAL OBSERVATION OF THERAPEUTIC EFFECT ON OCCIPITAL NEURALGIA TREATED WITH DAO FA IN ACUPUNCTURE MANIPULATION

    Institute of Scientific and Technical Information of China (English)

    冯军

    2008-01-01

    Objective To observe the clinical therapeutic effect of Dao Fa in acupuncture treatment of occipital neuralgia and make the comparison with routine acupuncture. Methods Dao Fa was applied to Tiānzhù (天柱BL10), Fēngchí (风池GB20) to treat occipital neuralgia and compare its results with routine acupuncture. Results The total effective rate was 95.6% in Dao Fa group, 77.8% in routine group, indicating significant difference (P<0.05). Conclusion Comparing with routine acupuncture, Dao Fa is advantageous at selection of fewer acupoints, better therapeutic effect and quicker achievement of efficacy in treatment of occipital neuralgia.

  1. Nanoparticles in paints: A new strategy to protect façades and surfaces?

    Science.gov (United States)

    Kaiser, J.-P.; Diener, L.; Wick, P.

    2013-04-01

    The paint and lacquer industries consider the use of nanosilver, photocatalytic active nanotitanium dioxide or nanosilica dioxide as additives for the protection of surfaces, against microbial, physical and chemical deterioration, as alternative to conventional organic based additives. Nowadays it is not clear, if nanoparticles in paints will achieve the proposed effects, since there are no long time studies available. Another fact is that the potential risks of nanoparticles for the environment and the human health is still controversial discussed. The most sensitive entry port for nanomaterials is the lung. However other human organs/systems may also be affected by nanoparticles. Therefore the aim of the study was to assess the potential hazard effects of the three most interesting particles for paints on the gastro-intestinal tract and the immune system in vitro. In our study we could show that: i) Nanosilver (TEM size 25 nm) was far less toxic than silver ions of comparable concentrations tested with cells representing the gastro-intestinal tract (CaCo-2) and immune cells (Jurkat, T-lymphocytes). A significant amount of necrotic cells could be observed after exposure of CaCo-2 cells to 27 μg/ml nanosilver for 48 h. ii) Nanotitanium dioxide can adsorb UV-light and in the presence of water hydroxyl radicals are generated photocatalytically. The exposure of CaCo-2 cells and Jurkat cells to photocatalytically active nanotitanium dioxide (Hombikat UV 100, TEM-size 15 nm) under dark conditions, didn't affected the cells significantly. However, the cells were able to incorporate nanotitanium dioxide, especially when cells were exposed to higher concentrations. iii) Nanosilica dioxide improves the properties of the paints by increasing the water repellence. When cells were exposed to 243 μg/ml nanosilica dioxide (TEM-size 19 nm) for up to 48 h no cytotoxic effect could be observed.

  2. 纤维溶解疗法治疗放射性臂丛损伤16例初步报告%Fibrinolytic therapy in radiation-induced brachial plexopathy:16 cases report and review

    Institute of Scientific and Technical Information of China (English)

    高庆国; 张连波; 高琛茂; 李树义; 刘军; 吴世峰

    2011-01-01

    Objective To explore the feasibility and curative effect of extracellular matrix (ECM) fibrinolytic therapy in order to cure radiation-induced brachial plexopathy (RIBP) and to discuss the principle, indication and precautions. Methods The treatment was taken on 16 cases that had a definite clinical diagnosis and we took a systematic examination to make sure that there was no recurrence or metastasis of the tumor.Brachial plexus and tender area block was taken once a week by injection of mixture of hyaluronidase, hexadecadrol, Vitamine B12 and lidocaine. The therapy was applied for 6 to 12 weeks. Results All the patients were followed up for a period of 0.5 to 10 years. Four cases had an evident alleviation of the symptoms and got a function resumption of hands; 5 cases had an alleviation of pain and improvement of sensation and the progress of illness were terminated, but motor function didn't have improvement; 7 cases had a continued aggravation of the symptoms. All the cases found improvement in electromyography. Concluslon The aim of fibrinolytic therapy is to dissolve the extra ECM such as hyaluronic acid around nerves in order to achieve a new homeostasis. It can relieve the conglutination and entrapment at nerve inside and outside, reduce the pressure of neuraxon and create an availing condition for the regenesis of nerve and the regain of function.%目的 探讨纤维溶解疗法治疗放射性臂丛损伤的可行性与疗效,讨论其原理、适应证及注意事项.方法对临床诊断明确、排除肿瘤复发、转移的16例放射性臂丛损伤患者采用纤维溶解疗法进行治疗,具体方法为使用透明质酸酶、地塞米松、维生素B12、2%利多卡因进行臂丛阻滞和局部压痛点阻滞,每周1次,连续6~12次.结果 随访0.5~10.0年,手运动和感觉功能明显改善、疼痛明显减轻4例,占25.00%;感觉改善、疼痛缓解、运动功能无改善、病情不再进一步发展5例,占31.25%;感觉、运动及

  3. EU-COST Aktion über „Nachhaltige Bekämpfung von Ambrosia artemisiifolia in Europa“ (COST FA1203-SMARTER: Chancen und Herausforderungen

    Directory of Open Access Journals (Sweden)

    Müller-Schärer, Heinz

    2014-07-01

    Full Text Available The EU -COST Action FA1203 on «Sustainable management of Ambrosia artemisiifolia in Europe (SMARTER” was successfully launched in February 2013 and will last for four years. Thirty-three countries have already signed the Memorandum of Understanding and over 180 researchers with specialists in weed research, invasive alien species management, ecology, aerobiology, allergology and economics are registered participants of SMARTER. COST Actions interlink nationally funded research projects and enable and finance conferences, working groups, training schools and research exchanges. SMARTER aims to initiate and develop long-term and sustainable control methods, to integrate these into existing mechanical and chemical control measures, and to quantify the success of these measures both for agriculture and health. The focus is on biological control methods with insects and fungi (especially using alien species from the area of origin of Ambrosia and vegetation management to achieve a competitive plant cover. For this, we develop and parameterize models, starting from the population dynamics of Ambrosia, on the impact of control measures on the frequency and distribution of Ambrosia and finally on pollen counts and allergy occurrences, each with both ecological and economic components. The necessary data are derived from the many experiments that we carry out in well-coordinated studies across Europe. SMARTER will allow the various stakeholders to select optimal habitat- and region-specific combinations of control methods. After an introduction and overview of the structure and the state of the Action, we briefly describe two planned activities typical for our Action, a study on the population dynamics of Ambrosia in different climates and habitats in Europe as a basis for estimating the efficiency of control measures, and an interdisciplinary study to clarify the impact the of North American native Ambrosia leaf beetle Ophraella communa (Coleoptera

  4. Telmisartan provides protection against development of impaired vasodilation independently of metabolic effects in SHRSP.Z-Lepr(fa)/IzmDmcr rats with metabolic syndrome.

    Science.gov (United States)

    Kagota, Satomi; Tada, Yukari; Nejime, Namie; Nakamura, Kazuki; Kunitomo, Masaru; Shinozuka, Kazumasa

    2011-05-01

    Metabolic syndrome is known to facilitate the development of cardiovascular disease. We have demonstrated that mesenteric arteries of SHRSP.Z-Lepr(fa)/IzmDmcr (SHRSP-fatty) rats with metabolic syndrome display an impaired vasorelaxation response mediated by nitric oxide. We examined whether the condition could be alleviated by treatment with telmisartan, an angiotensin II type 1 (AT1) receptor antagonist with PPAR-γ-activating properties and compared the results with those from pioglitazone, a PPAR-γ agonist. Telmisartan (5 mg·kg(-1)·day(-1)) or pioglitazone (2.5 mg·kg(-1)·day(-1)) was orally administered to male SHRSP-fatty rats for 8 weeks. Serum triglyceride and cholesterol levels were determined, and the oral glucose tolerance test was performed to evaluate insulin resistance. Vasodilations in response to acetylcholine and nitroprusside were determined by wire myographs under isometric tension conditions, protein expressions of soluble guanylyl cyclase in mesenteric arteries by Western blotting, and the contents of 3-nitrotyrosine in aortas by high-performance liquid chromatography with electrochemical detection. Telmisartan exerted antihypertensive effects, while pioglitazone ameliorated metabolic abnormalities in SHRSP-fatty rats. Telmisartan increased acetylcholine- and nitroprusside-induced relaxation and soluble guanylyl cyclase protein expression in mesenteric arteries and reduced 3-nitrotyrosine content in aortas. Pioglitazone displayed no such alleviating effects on vascular functions. These findings indicate that telmisartan protects against vasodilation disturbance through anti-oxidative and -nitrative stress independently of metabolic effects in SHRSP-fatty rats with metabolic syndrome.

  5. fA cellular automaton model of crystalline cellulose hydrolysis by cellulases

    Directory of Open Access Journals (Sweden)

    Little Bryce A

    2011-10-01

    Full Text Available Abstract Background Cellulose from plant biomass is an abundant, renewable material which could be a major feedstock for low emissions transport fuels such as cellulosic ethanol. Cellulase enzymes that break down cellulose into fermentable sugars are composed of different types - cellobiohydrolases I and II, endoglucanase and β-glucosidase - with separate functions. They form a complex interacting network between themselves, soluble hydrolysis product molecules, solution and solid phase substrates and inhibitors. There have been many models proposed for enzymatic saccharification however none have yet employed a cellular automaton approach, which allows important phenomena, such as enzyme crowding on the surface of solid substrates, denaturation and substrate inhibition, to be considered in the model. Results The Cellulase 4D model was developed de novo taking into account the size and composition of the substrate and surface-acting enzymes were ascribed behaviors based on their movements, catalytic activities and rates, affinity for, and potential for crowding of, the cellulose surface, substrates and inhibitors, and denaturation rates. A basic case modeled on literature-derived parameters obtained from Trichoderma reesei cellulases resulted in cellulose hydrolysis curves that closely matched curves obtained from published experimental data. Scenarios were tested in the model, which included variation of enzyme loadings, adsorption strengths of surface acting enzymes and reaction periods, and the effect on saccharide production over time was assessed. The model simulations indicated an optimal enzyme loading of between 0.5 and 2 of the base case concentrations where a balance was obtained between enzyme crowding on the cellulose crystal, and that the affinities of enzymes for the cellulose surface had a large effect on cellulose hydrolysis. In addition, improvements to the cellobiohydrolase I activity period substantially improved overall

  6. [Hydrolytic activity of microorganisms of the Dead Sea coastal ecosystems].

    Science.gov (United States)

    Varbanets', L D; Matseliukh, O V; Avdiiuk, K V; Hudzenko, O V; Nidialkova, N A; Romanovs'ka, V O; Tashirev, O B

    2014-01-01

    All strains tested are characterized by proteolytic (caseinolytic) activity, while elastase one was revealed only in two Gracilibacillus strains 6T2 and 7Tl. The activity was high enough (23.1 and 34.7 E/Ml, respectively). These values are at the level of bacterial producers which are described in literature: Bacillus mesentericus 316 M (6 E/Ml), Bacillus thuringiensis IMB B-7324 (50-55 E/Ml). The ability of two strains tested to synthesize enzyme, active against elastine, is important, so far as microbial enzyme may be perspective for using in medicine: elastases are able to dissociation of elastin fibres of connective tissues. These two strains display also fibrinolytic activity, however it was insignificant. Six of eight strains studied manifested alpha-amylase activity (0.01 - 1.173 E/Ml). It was shown that no strains, isolated from the Dead Sea costal ecosystems are able to manifest alpha-L-rhamnosidase activity.

  7. Finally feasible or fresh façade? Analyzing the internationalization plans of Japanese universities

    Directory of Open Access Journals (Sweden)

    Julian Chapple

    2014-05-01

    Full Text Available Recent policy moves in Japan reflect further attempts to internationalize the nation’s higher education sector. Building on previous plans, such as the goal of accepting 100,000 international students, Japan is now pinning its sights on a further increase (300,000, employing more foreign faculty, sending more Japanese students abroad to study, and teaching a greater number of classes in English. Falling under the broad auspices of the ‘Global 30’ national project, Japan hopes its forthcoming ‘Super Global Universities’ plan will see it achieve the goal of 10 of its universities in the world’s top 100. However, despite the slogans, the actual goals of the present plans appear decidedly domestic. The quality of higher education in Japan has long been a concern, and the nation’s desire for a more globally-active and competent workforce in face of a dramatically dwindling student population base are obvious. By using a mixed method approach to examine the content of recent plans, this paper seeks to ascertain what is really being sort after and whether it can be achieved. The above-mentioned four plans reveal little in terms of policy but speak volumes about Japan’s interpretation of the concept of internationalization. In particular, the widely held belief that teaching more classes in English is a remedy for the various present problems is looked at in detail. The paper concludes by examining what issues are overlooked in Japan’s discussion and what they reveal about the need for societal internationalization as a pre- or co-requisite for educational internationalization.

  8. Two NASA Dryden F/A-18's land on the Edwards Air Force Base runway after completion of an Autonomous

    Science.gov (United States)

    2001-01-01

    Two NASA Dryden F/A-18's land on the Edwards Air Force Base runway after completion of an Autonomous Formation Flight (AFF) mission. The goal of the AFF project is to demonstrate sustained 10 percent fuel savings by the trailing aircraft during cruise flight. Data suggests savings as high as 15 percent are achievable.

  9. Health-related quality of life in 794 patients with a peripheral facial palsy using the FaCE Scale: a retrospective cohort study.

    NARCIS (Netherlands)

    Kleiss, I.J.; Hohman, M.H.; Susarla, S.M.; Marres, H.A.M.; Hadlock, T.A.

    2015-01-01

    OBJECTIVES: To describe the health-related quality of life of patients visiting a tertiary referral centre for facial palsy, and to analyse factors associated with health-related quality of life, using the FaCE Scale instrument. DESIGN: Retrospective cohort study. SETTING: The Facial Nerve Center at

  10. Development and sensitivity study of a simplified and dynamic method for double glazing facade and verified by a full-scale façade element

    DEFF Research Database (Denmark)

    Liu, Mingzhe; Wittchen, Kim Bjarne; Heiselberg, Per

    2014-01-01

    with experimental data collected in a full-scale façade element test facility at Aalborg University (DK). Comparison was conducted between the simplified method and WIS software on the accuracy of calculating internal surface temperature of double glazing facade. The method is based on standards EN410 and EN673...

  11. Superparamagnetic Fe3O4-PEG2K-FA@Ce6 Nanoprobes for in Vivo Dual-mode Imaging and Targeted Photodynamic Therapy

    Science.gov (United States)

    Yin, Ting; Huang, Peng; Gao, Guo; Shapter, Joseph G.; Shen, Yulan; Sun, Rongjin; Yue, Caixia; Zhang, Chunlei; Liu, Yanlei; Zhou, Sui; Cui, Daxiang

    2016-11-01

    The development of targeted nanoprobes is a promising approach to cancer diagnostics and therapy. In the present work, a novel multifunctional photo/magnet-diagnostic nanoprobe (MNPs-PEG2K-FA@Ce6) has been developed. This nanoprobe is built using folic acid (FA), bifunctional polyethylene glycol (PEG2K) and photosensitizer chlorin e6 (Ce6). The MNPs-PEG2K-FA@Ce6 nanoprobes are superparamagnetic, can be synthesized on a large scale by a one-pot hydrothermal process without further surface modification and are stable in an aqueous environment for eight months. Compared with free Ce6 nanoprobes in vitro studies, the MNPs-PEG2K-FA@Ce6 nanoprobes significantly enhance cellular uptake efficiency and promote the effectiveness of photodynamic therapy (PDT) with the assistance of 633 nm laser irradiation. The unique nanoprobes show superior penetration and a retention time of more than six days with less accumulation in the liver allowing highly effective tumor recognition and monitoring. Additionally, there was little damage to healthy organs or tissues. These exciting new nanoprobes could be potential building blocks to develop new clinical therapies and translational medicine.

  12. Determination of ether extract digestibility and energy content of specialty lipids with variable FA and FFA content, and the effect of lecithin, for nursery pigs

    Science.gov (United States)

    Various specialty lipids are commercially available and used in nursery pig diets, but may have FA profiles and FFA content that affect their caloric value. In each of 2 experiments, 54 barrows (28-d of age) were fed a common diet for 7-d, allotted to dietary treatments and fed their respective expe...

  13. Deficiency of UBE2T, the E2 Ubiquitin Ligase Necessary for FANCD2 and FANCI Ubiquitination, Causes FA-T Subtype of Fanconi Anemia

    Directory of Open Access Journals (Sweden)

    Kimberly A. Rickman

    2015-07-01

    Full Text Available Fanconi anemia (FA is a rare bone marrow failure and cancer predisposition syndrome resulting from pathogenic mutations in genes encoding proteins participating in the repair of DNA interstrand crosslinks (ICLs. Mutations in 17 genes (FANCA-FANCS have been identified in FA patients, defining 17 complementation groups. Here, we describe an individual presenting with typical FA features who is deficient for the ubiquitin-conjugating enzyme (E2, UBE2T. UBE2T is known to interact with FANCL, the E3 ubiquitin-ligase component of the multiprotein FA core complex, and is necessary for the monoubiquitination of FANCD2 and FANCI. Proband fibroblasts do not display FANCD2 and FANCI monoubiquitination, do not form FANCD2 foci following treatment with mitomycin C, and are hypersensitive to crosslinking agents. These cellular defects are complemented by expression of wild-type UBE2T, demonstrating that deficiency of the protein UBE2T can lead to Fanconi anemia. UBE2T gene gains an alias of FANCT.

  14. Plekhs1 and Prdx3 are candidate genes responsible for mild hyperglycemia associated with obesity in a new animal model of F344-fa-nidd6 rat.

    Science.gov (United States)

    Kotoh, Jun; Sasaki, Daiki; Matsumoto, Kozo; Maeda, Akihiko

    2016-12-01

    Type 2 diabetes is a polygenic disease and characterized by hyperglycemia and insulin resistance, and it is strongly associated with obesity. However, the mechanism by which obesity contributes to onset of type 2 diabetes is not well understood. We generated rat strains with a hyperglycemic quantitative trait locus (QTL) derived from the Otsuka Long-Evans Tokushima Fatty rat and a fa/fa (Lepr(-/-)) locus derived from the Zucker Fatty rat. Phenotypes for plasma glucose, and insulin levels were measured, and RNA and protein levels were determined using reverse transcription quantitative PCR and Western blot analyses, respectively. Compared with the obese control strain F344-fa (Lepr(-/-)), plasma glucose levels of the obese F344-fa-nidd6 (Lepr(-/-) and Nidd6/of) significantly increased, and plasma insulin levels significantly decreased. These phenotypes were not observed in the lean strains, suggesting that the Nidd6/of locus harbors a diabetogenic gene associated with obesity. We measured the expression of 41 genes in the Nidd6/of QTL region of each strain and found that the mRNA expression levels of the two genes significantly differed between the obese strains. The two genes, pleckstrin homology domain-containing, family S member 1 (Plechs1) and peroxiredoxin III (Prdx3), were differentially expressed only in the obese rats, suggesting that these two genes are involved in the mild elevation of blood glucose levels and insulin resistance in obesity.

  15. Generation of human iPSC line GRX-MCiPS4F-A2 from adult peripheral blood mononuclear cells (PBMCs with Spanish genetic background

    Directory of Open Access Journals (Sweden)

    Sonia Cabrera

    2015-09-01

    Full Text Available We have generated iPSCs from peripheral blood mononuclear cells (PBMCs of a healthy man using heat sensitive and non-integrative Sendai virus containing Sox2, Oct3/4, c-Myc and Klf4. Human GRX-MCiPS4F-A2 cell line was established and characterized through this study.

  16. Isolation and differentiation of chondrocytic cells derived from human embryonic stem cells using dlk1/FA1 as a novel surface marker

    DEFF Research Database (Denmark)

    Harkness, Linda; Taipaleenmaki, Hanna; Mahmood, Amer

    2009-01-01

    was not expressed by undifferentiated hESC, but expressed during in vitro embryoid bodies (hEBs) formation upon down-regulation of undifferentiated markers e.g. Oct 3/4. Similarly, dlk1/FA1 was expressed in chondrocytic cells during in vivo teratoma formation. Interestingly, treatment of hEBs with Activin B...

  17. Effect of the time interval between fusion and activation on epigenetic reprogramming and development of bovine somatic cell nuclear transfer embryos.

    Science.gov (United States)

    Liu, Jun; Wang, Yongsheng; Su, Jianmin; Wang, Lijun; Li, Ruizhe; Li, Qian; Wu, Yongyan; Hua, Song; Quan, Fusheng; Guo, Zekun; Zhang, Yong

    2013-04-01

    Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence of SCNT embryos remain unknown. In the present study, the effects of different FA intervals (0 h, 2 h, and 4 h) on the epigenetic reprogramming and developmental competence of bovine SCNT embryos were assessed. The results demonstrated that H3 lysine 9 (H3K9ac) levels decreased rapidly after fusion in all three groups. H3K9ac was practically undetectable 2 h after fusion in the 2-h and 4-h FA interval groups. However, H3K9ac was still evidently detectable in the 0-h FA interval group. The H3K9ac levels increased 10 h after fusion in all three groups, but were higher in the 2-h and 4-h FA interval groups than that in the 0-h FA interval group. The methylation levels of the satellite I region in day-7 blastocysts derived from the 2-h or 4-h FA interval groups was similar to that of in vitro fertilization blastocysts and is significantly lower than that of the 0-h FA interval group. SCNT embryos derived from 2-h FA interval group showed higher developmental competence than those from the 0-h and 4-h FA interval groups in terms of cleavage rate, blastocyst formation rate, apoptosis index, and pregnancy and calving rates. Hence, the FA interval is an important factor influencing the epigenetic reprogramming and developmental competence of bovine SCNT embryos.

  18. Sediment grain-size data from sand augers collected in March/April and October 2014 from Assateague Island, Maryland (U.S. Geological Survey Field Activity Numbers [FAN] 2014-301-FA and 2014-322-FA)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The U.S. Geological Survey has a long history of responding to and documenting the impacts of storms along the Nation’s coasts and incorporating these data into...

  19. Sand auger and trench site locations collected in March/April and October 2014 from Assateague Island, Maryland (U.S. Geological Survey Field Activity Numbers [FAN] 2014-301-FA and 2014-322-FA)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The U.S. Geological Survey has a long history of responding to and documenting the impacts of storms along the Nation’s coasts and incorporating these data into...

  20. Data acquisition logs in PDF format maintained on U.S. Geological Survey Field Activities 2008-007-FA and 2008-037-FA in Great South Bay, Long Island, New York in May and September, 2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — An investigation of submarine aquifers adjacent to the Fire Island National Seashore and Long Island, New York, was conducted to assess the importance of submarine...