WorldWideScience

Sample records for fed brewers grain

  1. Production response of lactating cows fed dried versus wet brewers' grain in diets with similar dry matter content.

    Science.gov (United States)

    Dhiman, T R; Bingham, H R; Radloff, H D

    2003-09-01

    Twenty-four Holstein-Friesian dairy cows (20 intact and 4 fitted with rumen cannula) during early lactation (56 +/- 25.3 d in milk) were assigned to two treatments to determine intake and production responses to feeding dried and wet brewers' grain. There were two cows fitted with a rumen cannula in each treatment. Cows were fed a total mixed ration twice daily containing either dried or wet brewers' grain at 15% of the dietary dry matter (DM). The diet contained 47% forage and 53% concentrate. The experimental design was a replicated 2 x 2 Latin square with two periods of 5 wk each. First 2 wk in each period were considered as adaptation to diets and data from the last 3 wk were used for treatment comparisons. Dried and wet brewers' diets contained 68.0 and 66.5% DM, respectively. Feeding brewers' grain dry or wet to dairy cows had no influence on feed intake (25.6 vs. 25.1 kg/d), fat corrected milk yield (40.1 vs. 40.7 kg/d), milk composition and feed consumption. The pH, ammonia, total volatile fatty acids and molar ratios of volatile fatty acids in the rumen fluid were not different between treatments. Fatty acid composition of milk fat from cows fed diets containing dry or wet brewers' grain was identical, except C18:2 and C18:3 fatty acids were lower in milk fat from cows fed wet brewers' grain compared with dried brewers' grain. The results from the present study suggest that the performance of cows fed either dried or wet brewers' grain at 15% of dietary DM was similar when diets had the same DM. The average price for dried and wet brewers' grain in the United States from July 2001 to June 2002 was dollars 145.3 and dollars 96.9/metric tonne DM, respectively. Using wet instead of dried brewers' grain will save dollars 49/metric tonne minus the difference in storage costs. Wet brewers' grain can be fed to dairy cows in areas that are close to the brewery and provides nutritive value similar to the dried brewers' grain.

  2. Hydrolysis of Brewers' Spent Grain by Carbohydrate Degrading Enzymes

    NARCIS (Netherlands)

    Forssell, P.; Kontkanen, H.; Schols, H.A.; Hinz, S.W.A.; Eijsink, V.G.H.; Treimo, J.; Robertson, J.A.; Waldron, K.W.; Faulds, C.B.; Buchert, J.

    2008-01-01

    In this work four commercial cellulase-hemicellulase mixtures with different activity profiles were used for solubilization of carbohydrates from brewers' spent grain (BSG). After the enzyme treatment, both the solubilised fraction and the unhydrolysed residue were characterized. Treatment with

  3. Wet brewers grains for lactating dairy cows during hot, humid weather.

    Science.gov (United States)

    West, J W; Ely, L O; Martin, S A

    1994-01-01

    Twenty lactating Jersey cows were offered diets containing 0, 15, or 30% wet brewers grains or 30% wet brewers grains plus liquid brewers' yeast during hot, humid weather. The DMI was not different, even though diets with 30% wet brewers grains contained only 35.5% DM and approximately 50 versus 36.8% NDF for the control diet. Yields of milk and FCM did not differ for cows offered the control diet versus wet brewers grains or diets with 15 versus 30% wet brewers grains, but milk yield for diets with 30% wet brewers grains was greater with added liquid brewers' yeast than without it. Milk fat percentage was not different, but milk protein percentage was lower, for diets with wet brewers grains than for controls and for 30% wet brewers grains than for 15% wet brewers grains. Serum urea N was lower for control cows than for cows receiving the diets with wet brewers grains. Feed cost per cow was lower for wet brewers grains versus the control diet, and income over feed cost was greater for diets with 30 versus 15% wet brewers grains. Large quantities of wet brewers grains can be added to the diet during hot weather without depressing DMI.

  4. Protease-induced solubilisation of carbohydrates from brewers' spent grain

    NARCIS (Netherlands)

    Faulds, C.B.; Collins, S.; Robertson, J.A.; Treimo, J.; Eijsink, V.G.H.; Hinz, S.W.A.; Schols, H.A.; Buchert, J.; Waldron, K.W.

    2009-01-01

    The impact of microbial proteases on the release of carbohydrates from BSG was studied. The proteases were able to release the non-cellulosic glucose, a portion of feruloylated arabinoxylan and over 50% of the protein from brewers' spent grain (BSG) after 24 h hydrolysis. The non-cellulosic glucose

  5. Hydrolysis of Brewers' Spent Grain by Carbohydrate Degrading Enzymes

    NARCIS (Netherlands)

    Forssell, P.; Kontkanen, H.; Schols, H.A.; Hinz, S.W.A.; Eijsink, V.G.H.; Treimo, J.; Robertson, J.A.; Waldron, K.W.; Faulds, C.B.; Buchert, J.

    2008-01-01

    In this work four commercial cellulase-hemicellulase mixtures with different activity profiles were used for solubilization of carbohydrates from brewers' spent grain (BSG). After the enzyme treatment, both the solubilised fraction and the unhydrolysed residue were characterized. Treatment with 5,00

  6. Fractionation and Characterization of Brewers' Spent Grain Protein Hydrolysates

    OpenAIRE

    Celus, Inge; BRIJS, Kristof; Delcour, Jan

    2009-01-01

    Protein hydrolysates with a low and high degree of hydrolysis were enzymatically produced from brewers' spent grain (BSG), the insoluble residue of barley malt resulting from the manufacture of wort in the production of beer. To that end, BSG protein concentrate (BPC), prepared by alkaline extraction of BSG and subsequent acid precipitation, was enzymatically hydrolyzed with Alcalase during both 1.7 and 120 min. Because these hydrolysates contained many different peptides, fractionation of th...

  7. Corrected: The influence of brewers' yeast addition on lactic acid fermentation of brewers' spent grain hydrolysate by Lactobacillus rhamnosus

    OpenAIRE

    Pejin, Jelena; Radosavljević, Miloš; Kocić-Tanackov, Sunčica; Djukić-Vuković, Aleksandra; Mladenović, Dragana; Mojović, Ljiljana

    2016-01-01

    Brewers' spent grain (BSG) hydrolysates were used for lactic acid (LA) fermentation by Lactobacillus rhamnosus ATCC 7469. The aim of this study was to evaluate possibilities of the BSG hydrolysate utilization as a substrate for LA fermentation as well as the effect of dry brewers' yeast addition in hydrolysate on lactic acid fermentation parameters (L-(+)-LA and reducing sugar concentration and number of viable cell-viability). Very high L. rhamnosus ATCC 7469 cell viability was achieved in a...

  8. The influence of brewers' yeast addition on lactic acid fermentation of brewers' spent grain hydrolysate by Lactobacillus rhamnosus

    OpenAIRE

    Pejin, Jelena; Radosavljević, Miloš; Kocić-Tanackov, Sunčica; Đukić-Vuković, Aleksandra; Mladenović, Dragana; Mojović, Ljiljana

    2015-01-01

    In this study brewers' spent grain (BSG) hydrolysate was produced using optimal conditions. Hydrolysates were used for lactic acid fermentation by Lactobacillus rhamnosus ATCC 7469. The aim of this study was to evaluate possibilities of the BSG hydrolysate utilization as a substrate for lactic acid fermentation as well as the effect of dry brewers' yeast (1.0, 3.0, and 5.0 %) addition in hydrolysate on lactic acid fermentation parameters (L-(+)-lactic acid and reducing sugars concentration an...

  9. Microwave-Assisted Extraction of Brewers' Spent Grain Arabinoxylans

    OpenAIRE

    Rocha, M. A. M.; Saraiva, J.; Coimbra,M. A.

    2011-01-01

    Brewers´ spent grain (BSG) is a by-product from beer industry that can be exploited as a source of arabinoxylo-oligosaccharides (AXOS) with prebiotic activity. In this study, microwave-assisted extractions were performed during 2 min at 140-210°Cin order to evaluate the feasibility of this extraction technology for quantitative extraction of the arabinoxylans (AX) or AXOS from BSG. The AX yield increasedwith the increase of the temperature in the range used. The best condition...

  10. Digestibilidade da dieta, parâmetros ruminais e desempenho de ovinos Santa Inês alimentados com polpa cítrica peletizada e resíduo úmido de cervejaria Diet digestibility, ruminal parameters and performance of Santa Ines sheep fed dried citrus pulp and wet brewer grain

    Directory of Open Access Journals (Sweden)

    Susana Gilaverte

    2011-03-01

    mixture of wet brewer grain and citrus pulp. In experiment 1, for evaluation of intake, apparent digestibility of nutrients and ruminal parameters, 12 Santa Ines ram lambs fitted with canulas in the rumen were assigned to a randomized complete block design with three diets and four replicates. In experiment two, for performance evaluation, 48 ewe lambs were confined and fed the same diets of experiment 1. Replacement of corn by dried citrus pulp did not affect intake or digestibility of nutrients in the diet. However, the inclusion of wet brewer grain reduced intake and apparent digestibility of nutrients, with exception of NDF. Apparent digestibility of NDF was similar among diets. Inclusion of wet brewer grain in the diet decreased ruminal concentration of acetate and propionate and increased acetate/propionate ratio and ruminal pH. In general, there is no difference between the diet containing corn and that containing citrus pulp in total replacement to corn. The use of wet brewer grain, however, reduces average daily gain, final body weight and feed efficiency.

  11. Production of microbial enzymes using brewers spent grain

    Energy Technology Data Exchange (ETDEWEB)

    Okita, H.; Yamashita, H.; Yabuuchi, S.

    1985-01-01

    Brewers spent grain was used as the raw material for production of three extracellular enzymes, ..cap alpha..-amylase, alkaline protease, and lichenase, by Bacillus licheniformis. The basal medium was composed of wet spent grain (about 80% moisture) and 40 mM phosphate buffer, pH 7.0 (1:5-10, w/v). For ..cap alpha..-amylase production by strain No. 18, 0.5% corn oil was added to the basal medium, and the maximum activity (66 units/ml) was found in the culture solution of the 4th day of incubation at 45 degrees C. Alkaline protease from strain H-9 was produced in the basal medium supplemented with 2 mM CaCL/sub 2/ and 10mM sodium citrate. The maximum activity during incubation at 35 degrees C was 7.2 units/ml on the 4th day. On the other hand, lichenase was produced sufficiently in the basal medium by strain Y-25, and the activity reached the level of 20 units/ml on the 3rd day of incubation at 30 degrees C. This enzyme preparation could also hydrolyze ..beta..-glucans from malt and beer. 15 references.

  12. Brewer's spent grain: a valuable feedstock for industrial applications.

    Science.gov (United States)

    Mussatto, Solange I

    2014-05-01

    Brewer's spent grain (BSG) is the most abundant by-product generated from the beer-brewing process, representing approximately 85% of the total by-products obtained. This material is basically constituted by the barley grain husks obtained as solid residue after the wort production. Since BSG is rich in sugars and proteins, the main and quickest alternative for elimination of this industrial by-product has been as animal feed. However, BSG is a raw material of interest for application in different areas because of its low cost, large availability throughout the year and valuable chemical composition. In the last decade, many efforts have been directed towards the reuse of BSG, taking into account the incentive that has been given to recycle the wastes and by-products generated by industrial activities. Currently, many interesting and advantageous methods for application of BSG in foods, in energy production and in chemical and biotechnological processes have been reported. The present study presents and discusses the most recent perspectives for BSG application in such areas.

  13. Brewer's Grain from Cameroon Brewery in Breeder Chicken Rations : Effect on Productive and Reproductive Performance

    Directory of Open Access Journals (Sweden)

    Mafeni, MJ.

    2001-01-01

    Full Text Available In order to evaluate the effect of brewer's dried grain (BDG on the productive and reproductive traits in breeder chickens, 120 laying hens and 12 cocks of ISA commercial breed were subjected to dietary treatments containing 0, 10, 20, and 30 % levels of BDG. Feed and water were provided ad libitum over the 5-months experimental period. Reproductive and productive traits such as egg production, egg weight, albumen height, shell weight, semen quantity fertility and hatchability of fertile eggs were measured. Results indicated that when BDG was fed at the 30 % level in the ration, the hen-day egg production (50.6 % was significantly (P of inclusion. There was a significant (P 0.05 was noticed between treatments for ratio of shell weight to egg weight, albumen height, semen quantity and fertility. The results suggest that although the 30 % level of BDG can be tolerated, the 20 %, level of BDG inclusion is more appropriate for breeder birds.

  14. Synthesis Technology of PEI Modified Brewer's Grains for the Adsorption of Cr(VI

    Directory of Open Access Journals (Sweden)

    Yihua Jiang

    2015-05-01

    Full Text Available With beer production waste brewer's grains (BSG as the raw material, through polyethylenimine (PEI modified and glutaraldehyde (GA cross-linking process for preparing the modified brewer's grains biosorbents. The simulation of Cr (VI wastewater as treatment object, through the optimization of synthesis conditions of Cr (VI, the optimal synthesis process was obtained. The results showed, 4 g BSG and 100 mL 4% PEI methanol solution were placed in 250 mL conical flask at room temperature, shaking for 24 h. After the mixture,shifted to 200 mL l.5% GA solution directly, magnetic stirring for half an hour, the modified results of brewer’s grain was best. Under the optimal conditions, the average of adsorption rate was 98.82% and adsorption capacity was 45.31 mg/g. The modified brewer's grains of amine surfactant modified is a promising treatment of chromium wastewater biological materials.

  15. Fermentation applications of brewer' s spent grain%啤酒糟发酵应用

    Institute of Scientific and Technical Information of China (English)

    邹正; 陈力力; 王雅君; 廖杰琼

    2011-01-01

    啤酒糟是微生物发酵的良好基质,利用微生物发酵啤酒糟的用途非常广泛.啤酒糟的再利用,有利于保护生态环境和节约资源.综述了近年来国内外啤酒糟在酶制剂、动物饲料、活性物质、食品添加剂方面的发酵应用.%Brewer's spent grain was good resource for microorganism fermentation, which was widely utilized. Recycling brewer's spent grain was good for protecting ecological environment and saving resources. The applications of brewer's spent grain in enzyme preparation, animal feed, active substances and food additives from home and abroad were summarized.

  16. Activated carbon from pyrolysis of brewer's spent grain: Production and adsorption properties.

    Science.gov (United States)

    Vanreppelen, Kenny; Vanderheyden, Sara; Kuppens, Tom; Schreurs, Sonja; Yperman, Jan; Carleer, Robert

    2014-07-01

    Brewer's spent grain is a low cost residue generated by the brewing industry. Its chemical composition (high nitrogen content 4.35 wt.%, fibres, etc.) makes it very useful for the production of added value in situ nitrogenised activated carbon. The composition of brewer's spent grain revealed high amounts of cellulose (20.8 wt.%), hemicellulose (48.78 wt.%) and lignin (11.3 wt.%). The fat, ethanol extractives and ash accounted for 8.17 wt.%, 4.7 wt.% and 3.2 wt.%, respectively. Different activated carbons were produced in a lab-scale pyrolysis/activation reactor by applying several heat and steam activation profiles on brewer's spent grain. Activated carbon yields from 16.1 to 23.6 wt.% with high N-contents (> 2 wt.%) were obtained. The efficiency of the prepared activated carbons for phenol adsorption was studied as a function of different parameters: pH, contact time and carbon dosage relative to two commercial activated carbons. The equilibrium isotherms were described by the non-linear Langmuir and Freundlich models, and the kinetic results were fitted using the pseudo-first-order model and the pseudo-second-order model. The feasibility of an activated carbon production facility (onsite and offsite) that processes brewer's spent grain for different input feeds is evaluated based on a techno-economic model for estimating the net present value. Even though the model assumptions start from a rather pessimistic scenario, encouraging results for a profitable production of activated carbon using brewer's spent grain are obtained.

  17. Influence of graded levels of brewers dried grains on pellet quality and performance in broiler chickens.

    Science.gov (United States)

    Denstadli, V; Ballance, S; Knutsen, S H; Westereng, B; Svihus, B

    2010-12-01

    The aim of the current study was to evaluate the effect of a gradual substitution of wheat and soy with brewers dried grains (BDG) on pellet quality, performance, and organ weights in broiler chickens. Five diets were formulated in which 0, 10, 20, 30, or 40% BDG replaced wheat and soy, with a concomitant gradual reduction in the calculated AME level. Each of the 5 experimental diets was fed to 12-d-old broiler chickens (Ross 308) kept in 6 pens, with 12 birds/pen. The birds had ad libitum access to feed and water until termination of the experiment at d 33. Feed intake was not affected by BDG inclusion and compensatory feed intake did not occur, perhaps having been neutralized by a significant (P durability index. The pellet durability index was 85% in the control diet (0% BDG) and decreased significantly (P feed:gain ratio. The feed:gain ratio was significantly (P feed utilization, and favors the development of a well-functioning gizzard.

  18. Effect of on Rumen Fermentation Characteristics of Dried Brewers Grain, Methane Production and Bacterial Diversity

    Directory of Open Access Journals (Sweden)

    Alvin P. Soriano

    2014-11-01

    Full Text Available The effects of Lactobacillus mucosae (L. mucosae, a potential direct fed microbial previously isolated from the rumen of Korean native goat, on the rumen fermentation profile of brewers grain were evaluated. Fermentation was conducted in serum bottles each containing 1% dry matter (DM of the test substrate and either no L. mucosae (control, 1% 24 h broth culture of L. mucosae (T1, or 1% inoculation with the cell-free culture supernatant (T2. Each serum bottle was filled anaerobically with 100 mL of buffered rumen fluid and sealed prior to incubation for 0, 6, 12, 24, and 48 h from which fermentation parameters were monitored and the microbial diversity was evaluated. The results revealed that T1 had higher total gas production (65.00 mL than the control (61.33 mL and T2 (62.00 mL (p<0.05 at 48 h. Consequently, T1 had significantly lower pH values (p<0.05 than the other groups at 48 h. Ammonia nitrogen (NH3-N, individual and total volatile fatty acids (VFA concentration and acetate:propionate ratio were higher in T1 and T2 than the control, but T1 and T2 were comparable for these parameters. Total methane (CH4 production and carbon dioxide (CO2 were highest in T1. The percent DM and organic matter digestibilities were comparable between all groups at all times of incubation. The total bacterial population was significantly higher in T1 (p<0.05 at 24 h, but then decreased to levels comparable to the control and T2 at 48 h. The denaturing gradient gel electrophoresis profile of the total bacterial 16s rRNA showed higher similarity between T1 and T2 at 24 h and between the control and T1 at 48 h. Overall, these results suggest that addition of L. mucosae and cell-free supernatant during the in vitro fermentation of dried brewers grain increases the VFA production, but has no effect on digestibility. The addition of L. mucosae can also increase the total bacterial population, but has no significant effect on the total microbial diversity. However

  19. Performance of Clarias gariepinus Fed Dried Brewer's Yeast (Saccharomyces cerevisiae) Slurry in Replacement for Soybean Meal

    Science.gov (United States)

    Solomon, Shola Gabriel; Itodo, Gabriel Enemona

    2017-01-01

    Following disparity of earlier results, this study tested the performance of African catfish Clarias gariepinus fed dried brewer's yeast slurry meal (DBYM) based diets. Fingerlings of C. gariepinus with pooled mean initial weight of 1.58 ± 0.01 g were stocked in hapas (1 m × 1 m × 1 m) immersed in an earthen pond at a density of 15 fish per cage. Five diets with increasing substitution of soybean meal with 25%, 50%, 75%, and 100% of dried brewer's yeast and a control without dried brewer's yeast (0% substitution) were evaluated for 8 weeks. Palatability of diets reduced with increasing levels of DBYM. Growth and utilization parameters such as weight gain, feed conversion ratio, protein efficiency ratio, and specific growth rate differed significantly (p < 0.05) among treated groups. Specific growth rate decreased with increasing substitution while the best feed conversion ratio was obtained in the diet devoid of DBYM. Protein efficiency and utilization decreased with increasing levels of DBYM. Body composition was also affected by inclusion of DBYM with significant differences (p < 0.05) being observed across the diets. The trend in body composition follows the utilization of the diets. We conclude that the optimal range of inclusion and substitution of soybean meal with DBYM in C. gariepinus feed is between 1% and 14% of dry matter. PMID:28239492

  20. Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain

    Directory of Open Access Journals (Sweden)

    Stuart Wilkinson

    2016-06-01

    Full Text Available Enzyme saccharification of pretreated brewers spent grains (BSG was investigated, aiming at maximising glucose production. Factors investigated were; variation of the solids loadings at different cellulolytic enzyme doses, reaction time, higher energy mixing methods, supplementation of the cellulolytic enzymes with additional enzymes (and cofactors and use of fed-batch methods. Improved slurry agitation through aerated high-torque mixing offered small but significant enhancements in glucose yields (to 53 ± 2.9 g/L and 45% of theoretical yield compared to only 41 ± 4.0 g/L and 39% of theoretical yield for standard shaking methods (at 15% w/v solids loading. Supplementation of the cellulolytic enzymes with additional enzymes (acetyl xylan esterases, ferulic acid esterases and α-L- arabinofuranosidases also boosted achieved glucose yields to 58 – 69 ± 0.8 - 6.2 g/L which equated to 52 - 58% of theoretical yield. Fed-batch methods also enhanced glucose yields (to 58 ± 2.2 g/L and 35% of theoretical yield at 25% w/v solids loading compared to non-fed-batch methods. From these investigations a novel enzymatic saccharification method was developed (using enhanced mixing, a fed-batch approach and additional carbohydrate degrading enzymes which further increased glucose yields to 78 ± 4.1 g/L and 43% of theoretical yield when operating at high solids loading (25% w/v.

  1. Possible application of brewer's spent grain in biotechnology

    OpenAIRE

    Pejin Jelena D.; Radosavljević Miloš S.; Grujić Olgica S.; Mojović Ljiljana V.; Kocić-Tanackov Sunčica D.; Nikolić Svetlana B.; Đukić-Vuković Aleksandra J.

    2013-01-01

    Brewer’s spent grain is the major by-product in beer production. It is produced in large quantities (20 kg per 100 liters of produced beer) throughout the year at a low cost or no cost, and due to its high protein and carbohydrates content it can be used as a raw material in biotechnology. Biotechnological processes based on renewable agro-industrial by-products have ecological (zero CO2 emission, eco-friendly by-products) and economical (cheap raw materials and reduction of storage cos...

  2. Surveillance of Aflatoxin and Microbiota Related to Brewer's Grain Destined for Swine Feed in Argentina

    Directory of Open Access Journals (Sweden)

    Gisela A. Gerbaldo

    2011-01-01

    Full Text Available Córdoba province in the center of Argentina is an important area of swine production. The use of industry by-product (brewer's grain as feedstuff for swine is a regular practice and increases animal performance on these animals production. The occurrence of aflatoxin contamination is global, causing severe problems especially in developing countries. No reports on aflatoxin B1 production, micoflora, and potential aflatoxin B1 producing microorganism from brewer's grain are available. The aims of this study were (1 to isolate the microbiota species from brewer's grain, (2 to determine aflatoxin B1 natural contamination levels, and (3 to determine the ability of Aspergillus section Flavi isolates to produce aflatoxins in vitro. Physical properties, total fungal counts, lactic acid bacteria, and fungal genera distribution were determined on this substrate. In 65% of the samples, fungal counts were higher than recommended by GMP, and lactic bacterium counts ranged from 1.9×105 to 4.4×109 CFU g−1. Aspergillus spp. prevailed over other fungal genera. Aspergillus flavus was the prevalent species followed by A. fumigatus. Aflatoxin B1 levels in the samples were higher than the recommended limits (20 ng g−1 for complementary feedstuffs. Several Aspergillus section Flavi strains were able to produce aflatoxin B1  in vitro. Inadequate storage conditions promote the proliferation of mycotoxin-producing fungal species. Regular monitoring of feeds is required in order to prevent chronic and acute toxic syndromes related to this kind of contamination.

  3. Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate

    Directory of Open Access Journals (Sweden)

    Adriana Knob

    2013-01-01

    Full Text Available In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+ and the reducing agents β-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+ as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.

  4. Production, purification, and characterization of a major Penicillium glabrum xylanase using Brewer's spent grain as substrate.

    Science.gov (United States)

    Knob, Adriana; Beitel, Susan Michelz; Fortkamp, Diana; Terrasan, César Rafael Fanchini; de Almeida, Alex Fernando

    2013-01-01

    In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn(2+) and the reducing agents β -mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg(2+), Zn(2+), and Cu(2+) as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.

  5. Evaluation of corn distillers dried grains with solubles and brewers yeast in diets for channel catfish Ictalurus punctatus

    Science.gov (United States)

    A study was conducted to examine the use of distillers grains with solubles (DDGS), ethanol extracted DDGS (EDDGS), and brewers yeast in channel catfish, Ictalurus punctatus, diets. Diets containing these ingredients were compared with all-plant and fish meal control diets. Juvenile channel catfish ...

  6. Carboxylic acid production from brewer's spent grain via mixed culture fermentation.

    Science.gov (United States)

    Liang, Shaobo; Wan, Caixia

    2015-04-01

    This study aimed at investigating carboxylic acid production from brewer's spent grain (BSG) via mixed culture fermentation. The results showed that the distribution of fermentation products was significantly affected by pH conditions and the addition of electron donors. Lactic acid was the dominant component under acidic and alkaline conditions while volatile fatty acids (VFAs) became dominant under the neutral condition. Furthermore, the neutral condition favored the chain elongation of carboxylic acids, especially with ethanol as the electron donor. Ethanol addition enhanced valeric acid and caproic acid production by 44% and 167%, respectively. Lactic acid addition also had positive effects on VFAs production under the neutral condition but limited to C2-C4 products. As a result, propionic acid and butyric acid production was increased by 109% and 152%, respectively. These findings provide substantial evidence for regulating carboxylic acid production during mixed culture fermentation of BSG by controlling pH and adding electron donors.

  7. Interactions of a lignin-rich fraction from brewer's spent grain with gut microbiota in vitro.

    Science.gov (United States)

    Niemi, Piritta; Aura, Anna-Marja; Maukonen, Johanna; Smeds, Annika I; Mattila, Ismo; Niemelä, Klaus; Tamminen, Tarja; Faulds, Craig B; Buchert, Johanna; Poutanen, Kaisa

    2013-07-10

    Lignin is a constituent of plant cell walls and thus is classified as part of dietary fiber. However, little is known about the role of lignin in gastrointestinal fermentation. In this work, a lignin-rich fraction was prepared from brewer's spent grain and subjected to an in vitro colon model to study its potential bioconversions and interactions with fecal microbiota. No suppression of microbial conversion by the fraction was observed in the colon model, as measured as short-chain fatty acid production. Furthermore, no inhibition on the growth was observed when the fraction was incubated with strains of lactobacilli and bifidobacteria. In fact, the lignin-rich fraction enabled bifidobacteria to survive longer than with glucose. Several transiently appearing phenolic compounds, very likely originating from lignin, were observed during the fermentation. This would indicate that the gut microbiota was able to partially degrade lignin and metabolize the released compounds.

  8. Enhancement of biohydrogen production from brewers' spent grain by calcined-red mud pretreatment.

    Science.gov (United States)

    Zhang, Jishi; Zang, Lihua

    2016-06-01

    This paper investigated the utilization of calcined-red mud (CRM) pretreatment to enhance fermentative hydrogen yields from brewers' spent grain (BSG). The BSG samples were treated with different concentrations (0.0-20g/L) of CRM at 55°C for 48h, before the biohydrogen process with heat-treated anaerobic sludge inoculum. The highest specific hydrogen production of 198.62ml/g-VS was obtained from the BSG treated with 10g/L CRM, with the corresponding lag time of 10.60h. Hydrogen yield increments increased by 67.74%, compared to the control tests without CRM. The results demonstrated that the CRM could hydrolyze more cellulose and further provided adequate broth and suitable pH value for efficient fermentative hydrogen. The model-based analysis showed that the modified Gompertz model presented a better fit for the experimental data than the first-order model.

  9. Effects of structure and xylanase treatment of brewers' spent grain on performance and nutrient availability in broiler chickens.

    Science.gov (United States)

    Denstadli, V; Westereng, B; Biniyam, H G; Ballance, S; Knutsen, S H; Svihus, B

    2010-06-01

    1. A factorial (2 x 3) feeding trial was set up to investigate the effects of coarse or finely ground brewers' spent grain (BSG) and xylanase treatment, either with no xylanase, top-dressed with xylanase or pre-treated with xylanase. 2. The experimental diets shared the same basal formulation and were fed to male broiler chickens (Ross 308) housed in individual cages from 12 to 29 d of age. 3. Xylanase pre-treatment reduced the dietary concentration of arabinoxylan by 15-30%. Pellet durability increased when BSG was ground. 4. Feed utilisation was significantly higher (6%) when the birds were given coarse BSG rather than ground BSG, whereas there was no significant effect of enzyme treatment. Apparent metabolisable energy was unaffected by the dietary treatments. 5. The overall starch digestibility was high (99%), with no dietary differences, whereas ileal protein digestibility was low (57%). Xylanase top-dressing tended to improve ileal protein digestibility but, in general, xylanase treatment had no major effect on overall performance in male broilers given diets with BSG.

  10. Enzymatic hydrolysis of beer brewers' spent grain and the influence of pretreatments

    Energy Technology Data Exchange (ETDEWEB)

    Beldman, G.; Hennekam, J.; Voragen, A.G.J.

    1987-01-01

    The enzymatic saccharification of plant material has been shown to be of interest in various fields, such as the production of fruit juices and the utilization of biomass. A combination of cellulase, pectinase, and hemicellulases is usually used because of the chemical composition of the matrix of plant cell walls. For apples, beet pulp, and potato fiber, almost a complete hydrolysis of polysaccharides is obtained by combining cellulase and pectinase. For nonparenchymatic tissue, the situation is somewhat different: pectin is a minor component and the hemicellulose content is much higher. Enzyme action is restricted by the lignin barrier and by the high crystallinity of cellulose in this material. For such materials, mechanical, thermal, or chemical pretreatments are necessary to achieve efficient hydrolysis. This communication describes various enzymatic treatments and chemical and physical pretreatment, using brewers' spent grain as substrate. Spent grain is the residue of malt and grain which remains in the mash-kettle after the liquefied and saccharified starch has been removed by filtration. (Refs. 15).

  11. Evaluation of brewers' spent grain as a novel media for yeast growth.

    Science.gov (United States)

    Cooray, Sachindra T; Lee, Jaslyn J L; Chen, Wei Ning

    2017-12-01

    Brewers' spent grain (BSG) is a by-product generated from the beer manufacturing industry, which is extremely rich in protein and fiber. Here we use low cost BSG as the raw material for the production of a novel growth media, through a bioconversion process utilizing a food grade fungi to hydrolyze BSG. The novel fermentation media was tested on the yeast Rhodosporidium toruloides, a natural yeast producing carotenoid. The yeast growth was analysed using the growth curve and the production of intracellular fatty acids and carotenoids. Untargeted GCMS based metabolomics was used to analyse the constituents of the different growth media, followed by multivariate data analysis. Growth media prepared using fermented BSG was found to be able to support the growth in R. toruloides (21.4 mg/ml) in comparable levels to YPD media (24.7 mg/ml). Therefore, the fermented BSG media was able to fulfill the requirement as a nitrogen source for R. toruloides growth. This media was able to sustain normal metabolomics activity in yeast, as indicated by the level of fatty acid and carotenoid production. This can be explained by the fact that, in the fermented BSG media metabolites and amino acids were found to be higher than in the unfermented media, and close to the levels in YPD media. Taken together, our study provided evidence of a growth media for yeast using BSG. This should have potential in replacing components in the current yeast culture media in a sustainable and cost effective manner.

  12. Production, Purification, and in Vitro Evaluation of the Prebiotic Potential of Arabinoxylooligosaccharides from Brewer's Spent Grain.

    Science.gov (United States)

    Gómez, Belén; Míguez, Beatriz; Veiga, Adán; Parajó, Juan Carlos; Alonso, José Luís

    2015-09-30

    Brewer's spent grain (BSG) samples were subjected to a two-step aqueous processing (starch extraction and autohydrolysis) in order to assess their potential as a raw material for obtaining a mixture of arabinoxylooligosaccharides (AXOS) suitable to be use as prebiotics for elderly. After hydrothermal treatment, the liquors were refined by a sequence of purification and conditioning steps including membrane filtration, enzymatic hydrolysis, and ion exchange. The presence of both substituted (degree of polimerization (DP) = 2-10) and unsubstituted (DP = 2-16) oligosaccharides made up of xylose and arabinose (AXOS) were confirmed in purified mixtures (in which total OS content = 84% w/w) by using chromatographic techniques and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Finally, AXOS were evaluated for their prebiotic activity by in vitro fermentation assays using fecal inocula from elderly people, demonstrating that AXOS were slightly better substrates than FOS, in terms of bacterial population shifts as in the production of SCFA.

  13. Techno-economic analysis for brewer's spent grains use on a biorefinery concept: the Brazilian case.

    Science.gov (United States)

    Mussatto, Solange I; Moncada, Jonathan; Roberto, Inês C; Cardona, Carlos A

    2013-11-01

    A techno-economic analysis for use of brewer's spent grains (BSG) on a biorefinery concept for the Brazilian case is presented. Four scenarios based on different levels of heat and mass integration for the production of xylitol, lactic acid, activated carbon and phenolic acids are shown. A simulation procedure using the software Aspen Plus and experimental yields was used. Such procedure served as basis for the techno-economic and environmental assessment according to the Brazilian conditions. Full mass integration on water and full energy integration was the configuration with the best economic and environmental performance. For this case, the obtained economic margin was 62.25%, the potential environmental impact was 0.012 PEI/kg products, and the carbon footprint of the processing stage represented 0.96 kg CO2-e/kg of BSG. This result served as basis to draw recommendations on the technological, economic and environmental feasibility for implementation of such type of biorefinery in Brazil.

  14. The biotransformation of brewer's spent grain into biogas by anaerobic microbial communities.

    Science.gov (United States)

    Malakhova, Dina V; Egorova, Maria A; Prokudina, Ljuba I; Netrusov, Alexander I; Tsavkelova, Elena A

    2015-12-01

    The present study reports on the biotransformation of the brewer's spent grain (BSG) in co-digestion with Jerusalem artichoke (JA, Helianthus tuberosus L.) phytomass by thermophilic (+55 °C) and mesophilic (+30 °C) anaerobic methanogenic communities. BSG is a by-product of the beer-brewing process generated in large amounts, in which utilization provokes a negative effect on the environment. In this study, we will show an effective conversion of BSG into biogas by selected microbial communities, obtained from different sources (animal manure and previously isolated microbial consortia). The stimulation of methanogenesis was reached by the co-digestion of JA's phytomass (stem and leaves). The optimized conditions for microbial stable cultivation included the use of nutrient medium, containing yeast extract and trace element solution. The optimal BSG concentration in biogas production was 50 and 100 g L(-1). Under thermophilic conditions, the maximum total methane production reached 64%, and it comprised around 6-8 and 9-11 of L CH4 per 100 g of fermented BSG without and with co-digested JA, respectively, when the fresh inoculum was added. Although, after a year of re-cultivation, the values reduced to around 6-7, and 6-10 L CH4/100 g BSG, correspondingly, the selected microbial communities showed effective biotransformation of BSG. The supplementation of soil with the residual fermented BSG (10%, w/w) resulted in the promotion of lettuce (Lepidium sativum L.) growth. The results obtained demonstrate a potential for complete BSG utilization via biogas production and application as a soil additive.

  15. Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3

    Directory of Open Access Journals (Sweden)

    Rodrigo Pires do Nascimento

    2011-12-01

    Full Text Available Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.

  16. Enhanced ethanol production from brewer's spent grain by a Fusarium oxysporum consolidated system

    Directory of Open Access Journals (Sweden)

    Christakopoulos Paul

    2009-02-01

    Full Text Available Abstract Background Brewer's spent grain (BG, a by-product of the brewing process, is attracting increasing scientific interest as a low-cost feedstock for many biotechnological applications. BG in the present study is evaluated as a substrate for lignocellulolytic enzyme production and for the production of ethanol by the mesophilic fungus Fusarium oxysporum under submerged conditions, implementing a consolidated bioconversion process. Fermentation experiments were performed with sugar mixtures simulating the carbohydrate content of BG in order to determine the utilization pattern that could be expected during the fermentation of the cellulose and hemicellulose hydrolysate of BG. The sugar mixture fermentation study focused on the effect of the initial total sugar concentration and on the effect of the aeration rate on fermenting performance of F. oxysporum. The alkali pretreatment of BG and different aeration levels during the ethanol production stage were studied for the optimization of the ethanol production by F. oxysporum. Results Enzyme yields as high as 550, 22.5, 6.5, 3225, 0.3, 1.25 and 3 U per g of carbon source of endoglucanase, cellobiohydrolase, β-D-glucosidase, xylanase, feruloyl esterase, β-D-xylosidase and α-L-arabinofuranosidase respectively, were obtained during the growth stage under optimized submerged conditions. An ethanol yield of 109 g ethanol per kg of dry BG was obtained with alkali-pretreated BG under microaerobic conditions (0.01 vvm, corresponding to 60% of the theoretical yield based on total glucose and xylose content of BG. Conclusion The enzymatic profile of the extracellular extract from F. oxysporum submerged cultures using BG and corn cob as the carbon source was proved efficient for a successful hydrolysis of BG. The fermentation study carried out using sugar mixtures simulating BG's carbohydrates content and consecutively alkali-pretreated and untreated BG, indicates that BG hydrolysis is the bottleneck

  17. Transcriptomic profiling of spleen in grass-fed and grain-fed Angus cattle

    Science.gov (United States)

    The grass-fed cattle obtain nutrients directly from pastures containing limited assimilable energy but abundant amount of fiber; by contrast, grain-fed steers receive a diet that is comprised mainly of grains and serves as an efficient source of high-digestible energy. Besides energy, these two type...

  18. The effects on cow performance and calf birth and weaning weight of replacing grass silage with brewers grains in a barley straw diet offered to pregnant beef cows of two different breeds.

    Science.gov (United States)

    Rooke, J A; Duthie, C-A; Hyslop, J J; Morgan, C A; Waterhouse, T

    2016-08-01

    The effects on cow and calf performance of replacing grass silage with brewers grains in diets based on barley straw and fed to pregnant beef cows are reported. Using a 2 × 2 factorial arrangement of breed and diet, cows pregnant by artificial insemination (n = 34) of two breeds (cross-bred Limousin, n = 19 and pure-bred Luing, n = 15) were fed diets ad libitum which consisted of either (g/kg dry matter) barley straw (664) and grass silage (325; GS) or barley straw (783) and brewers grains (206, BG) and offered as total mixed rations. From gestation day (GD) 168 until 266, individual daily feed intakes were recorded and cow body weight (BW) and body condition score (BCS) measured weekly. Calving date, calf sex, birth and weaning BW, and calf age at weaning were also recorded. Between GD 168 and 266, cross-bred Limousin cows gained more weight than Luing cows (p < 0.05) and cows offered BG gained more weight than cows offered GS (p < 0.001). Luing cows lost more BCS than cross-bred Limousin cows (p < 0.05), but diet did not affect BCS. There were no differences in dry matter intake as a result of breed or diet. Calf birth BW, however, was greater for cows fed BG than GS (44 vs. 38 kg, SEM 1.0, p < 0.001) with no difference between breeds. At weaning, calves born to BG-fed cows were heavier than those born to GS-fed cows (330 vs. 286 kg, SEM 9.3, p < 0.01). In conclusion, replacement of grass silage with brewers grains improved the performance of beef cows and increased calf birth and weaning BW. Further analysis indicated that the superior performance of cows offered the BG diet was most likely due to increases in protein supply which may have improved both energy and protein supply to the foetus.

  19. Decreasing the frequency and rate of wet brewers grains supplementation did not impact growth but reduced humoral immune response of preconditioning beef heifers.

    Science.gov (United States)

    Moriel, P; Piccolo, M B; Artioli, L F A; Poore, M H; Marques, R S; Cooke, R F

    2016-07-01

    This study evaluated growth and measurements of innate and humoral immunity of preconditioning beef heifers supplemented with wet brewers grains (WBG) at 2 supplementation rates and frequencies. At 14 d after weaning (d 0), Angus heifers ( = 36; 213 ± 2 kg BW and 254 ± 7 d of age) were stratified by BW and age and randomly assigned to 1 of 12 drylot pens (3 heifers/pen). Treatments were randomly assigned to pens, in a 2 × 2 factorial design, and consisted of heifers provided ground tall fescue hay ad libitum (55% TDN and 12% CP of DM) and supplemented with WBG (75% TDN and 36% CP of DM) either daily (7X) or 3 times weekly (3X; Monday, Wednesday, and Friday) at 0.5 or 1.0% of BW (DM basis) for 42 d. Heifers were vaccinated against infectious bovine rhinotracheitis (IBR), bovine viral diarrhea virus (BVDV), Mannheimia haemolytica, and Clostridium on d 14 and 28. Individual BW was measured before feeding on d 0 and 42 following 12 h of feed and water withdrawal. Blood samples were collected via jugular venipuncture 4 h after WBG supplementation on d 14, 15, 16, 17, 21, 28, 29, 30, 31, 35, and 42. Heifers fed WBG 3X had less hay DMI (2.6 ± 0.16 vs. 3.2 ± 0.16 kg/d; against BVDV-2 and IBR were greater for heifers fed WBG at 1.0% of BW vs. heifers fed WBG at 0.5% of BW (7.6 vs. 6.7 and 3.3 vs. 2.8 ± 0.19 log, respectively). In summary, decreasing WBG supplementation frequency (7 vs. 3 times weekly) or rate (1.0 vs. 0.5% of BW) for recently weaned beef heifers did not affect growth but decreased vaccine-induced antibody production against pathogens associated with bovine respiratory disease during a 42-d preconditioning period.

  20. Fermentation efficiency of cells immobilized on delignified brewers' spent grains after low- and high-temperature thin layer thermal drying.

    Science.gov (United States)

    Tsaousi, Konstantina; Koutinas, Athanasios A; Bekatorou, Argyro; Loukatos, Paul

    2010-09-01

    Low-cost dried yeasts immobilized on delignified brewers' spent grains for use in wine making and brewing were produced by simple thermal drying techniques. To optimize the thermal drying process, vacuum and air stream conditions were examined. Drying of thin layers of the biocatalysts was performed at low (30-38 degrees C) and high temperatures (40-70 degrees C). The fermentation efficiency of the thermally dried biocatalysts was acceptable, with immobilized cells showing a significantly higher thermotolerance compared with free cells. Immobilized cells dried at high temperatures presented slightly improved glucose fermentation efficiency compared with the low-temperature dried biocatalysts. Gas chromatography-mass spectrometry analysis of aroma volatiles of the fermented products revealed an increase of esters, lower higher alcohol formation, and significantly lower concentration of carbonylic compounds.

  1. The Content in Bioactive Compounds of Different Brewers' Spent Grain Aqueous Extracts

    OpenAIRE

    Anca FARCAS; Sonia SOCACI; Maria TOFANA; Elena MUDURA; Salanta, Liana

    2016-01-01

    In the last decade by-products of food and beverage processing have attracted much attention due to their functionality and potential as food ingredients. Brewers’ spent grain is the major by-product of the brewing industry representing a valuable source of bioactive ingredients. The aim of this study was to assess the effect of extraction time and temperature on the efficiency of water as solvent for the extraction of bioactive compounds from brewers’ spent grain (BSG). In terms of extractio...

  2. Selection of the Strain Lactobacillus acidophilus ATCC 43121 and Its Application to Brewers' Spent Grain Conversion into Lactic Acid

    Science.gov (United States)

    Liguori, Rossana; Soccol, Carlos Ricardo; Vandenberghe, Luciana Porto de Souza; Woiciechowski, Adenise Lorenci; Ionata, Elena; Marcolongo, Loredana; Faraco, Vincenza

    2015-01-01

    Six Lactobacillus strains were analyzed to select a bacterium for conversion of brewers' spent grain (BSG) into lactic acid. Among the investigated strains, L. acidophilus ATCC 43121 showed the highest yield of lactic acid production (16.1 g/L after 48 hours) when grown in a synthetic medium. It was then analyzed for its ability to grow on the hydrolysates obtained from BSG after acid-alkaline (AAT) or aqueous ammonia soaking (AAS) pretreatment. The lactic acid production by L. acidophilus ATCC 43121 through fermentation of the hydrolysate from AAS treated BSG was 96% higher than that from the AAT treated one, although similar yields of lactic acid per consumed glucose were achieved due to a higher (46%) glucose consumption by L. acidophilus ATCC 43121 in the AAS BSG hydrolysate. It is worth noting that adding yeast extract to the BSG hydrolysates increased both the yield of lactic acid per substrate consumed and the volumetric productivity. The best results were obtained by fermentation of AAS BSG hydrolysate supplemented by yeast extract, in which the strain produced 22.16 g/L of lactic acid (yield of 0.61 g/g), 27% higher than the value (17.49 g/L) obtained in the absence of a nitrogen source. PMID:26640784

  3. A novel application of microwave-assisted extraction of polyphenols from brewer's spent grain with HPLC-DAD-MS analysis.

    Science.gov (United States)

    Moreira, Manuela M; Morais, Simone; Barros, Aquiles A; Delerue-Matos, Cristina; Guido, Luís F

    2012-05-01

    This paper reports a novel application of microwave-assisted extraction (MAE) of polyphenols from brewer's spent grains (BSG). A 2(4) orthogonal composite design was used to obtain the optimal conditions of MAE. The influence of the MAE operational parameters (extraction time, temperature, solvent volume and stirring speed) on the extraction yield of ferulic acid was investigated through response surface methodology. The results showed that the optimal conditions were 15 min extraction time, 100 °C extraction temperature, 20 mL of solvent, and maximum stirring speed. Under these conditions, the yield of ferulic acid was 1.31 ± 0.04% (w/w), which was fivefold higher than that obtained with conventional solid-liquid extraction techniques. The developed new extraction method considerably reduces extraction time, energy and solvent consumption, while generating fewer wastes. HPLC-DAD-MS analysis indicated that other hydroxycinnamic acids and several ferulic acid dehydrodimers, as well as one dehydrotrimer were also present, confirming that BSG is a valuable source of antioxidant compounds.

  4. Steam explosion of Brewer's spent grain improves enzymatic digestibility of carbohydrates and affects solubility and stability of proteins.

    Science.gov (United States)

    Kemppainen, K; Rommi, K; Holopainen, U; Kruus, K

    2016-09-01

    Steam explosion was studied as a means to improve the enzymatic digestibility of carbohydrates in Brewer's spent grain, a protein and lipid-rich lignocellulosic by-product of the brewing industry. Having temperature, treatment time and the presence of acid catalyst as variables, a treatment at 200 °C for 10 min without an acid catalyst was found to be the most efficient, dissolving 12.1 % of the dry matter. Mainly oligomeric non-cellulosic glucan and arabinoxylan were dissolved, and the remaining insoluble carbohydrates could be efficiently hydrolysed by an enzyme cocktail (75 % hydrolysis yield). The process also caused partial protein degradation and dissolved over a third of the total nitrogen. Meanwhile, the insoluble protein appeared to become more strongly associated with acid-insoluble lignin. Compositional changes observed in the proteins and carbohydrates were supported by the results of epifluorescence microscopy. The process yielded three chemically different fractions which could serve as biorefinery products or intermediates.

  5. Removal of the Pesticide Pymetrozine from Aqueous Solution by Biochar Produced from Brewer's Spent Grain at Different Pyrolytic Temperatures

    Directory of Open Access Journals (Sweden)

    Xinguo Xi

    2014-10-01

    Full Text Available Biochar (BC produced from brewer's spent grain (BSG via slow pyrolysis at 300, 400, 500, 600, and 700 °C was characterized and investigated as an adsorbent for the removal of the pesticide pymetrozine from aqueous solution. Batch BSG BCs adsorption experiments were carried out under various conditions (such as pH, pymetrozine concentration, and BC dosage to adsorb the pymetrozine. The BSG BCs adsorption pymetrozine capacities were increased by 21.4% to 55.5% under pyrolysis temperatures of 300, 400, 500, and 600 °C compared to 700 °C with a pyrolysis time of 2 h and by 19.0% to 52.1% at 4 h. At solution pH values of 2, 4, 6, and 8, the adsorption capacities were increased by 9.6% to 39.5% compared with that at pH 10. A similar adsorption tendency was found for the different BCs dosage. In the first 60 min, BC absorbed 70% to 80% pymetrozine. The Langmuir and Freundlich model were highly correlated with BC adsorption. The magnitude of free energy decreased by 32.2% to 47.3% with increasing temperature. The value of the enthalpy change showed the adsorption to be endothermic. The BSG BC had high efficiency in adsorbing pymetrozine and had great potential to prevent the water pollution and reuse the waste of the beer factory.

  6. Selection of the Strain Lactobacillus acidophilus ATCC 43121 and Its Application to Brewers' Spent Grain Conversion into Lactic Acid.

    Science.gov (United States)

    Liguori, Rossana; Soccol, Carlos Ricardo; Vandenberghe, Luciana Porto de Souza; Woiciechowski, Adenise Lorenci; Ionata, Elena; Marcolongo, Loredana; Faraco, Vincenza

    2015-01-01

    Six Lactobacillus strains were analyzed to select a bacterium for conversion of brewers' spent grain (BSG) into lactic acid. Among the investigated strains, L. acidophilus ATCC 43121 showed the highest yield of lactic acid production (16.1 g/L after 48 hours) when grown in a synthetic medium. It was then analyzed for its ability to grow on the hydrolysates obtained from BSG after acid-alkaline (AAT) or aqueous ammonia soaking (AAS) pretreatment. The lactic acid production by L. acidophilus ATCC 43121 through fermentation of the hydrolysate from AAS treated BSG was 96% higher than that from the AAT treated one, although similar yields of lactic acid per consumed glucose were achieved due to a higher (46%) glucose consumption by L. acidophilus ATCC 43121 in the AAS BSG hydrolysate. It is worth noting that adding yeast extract to the BSG hydrolysates increased both the yield of lactic acid per substrate consumed and the volumetric productivity. The best results were obtained by fermentation of AAS BSG hydrolysate supplemented by yeast extract, in which the strain produced 22.16 g/L of lactic acid (yield of 0.61 g/g), 27% higher than the value (17.49 g/L) obtained in the absence of a nitrogen source.

  7. Effective utilization of heat from incinerating brewer prime s grains and dehydrated sludge. Beer kasu dassui odei shokyakunetsu no yuko riyo

    Energy Technology Data Exchange (ETDEWEB)

    Ogawa, T. (Asahi Breweries, Ltd., Tokyo (Japan))

    1991-02-04

    Merits of treatment cost reduction and effective utilization of waste heat were obtained through effectively utilizing the waste heat from the in-plant incineration of the brewer {prime} s grains (barley grains) generated from the brewing processes, and the dehydrated sludge generated from the waste water treatment. Keeping the pace with increase in the annual manufacturing capacity at Hakata Plant, from 60,000 kl to 180,000 kl, the brewer {prime} s grain generation has increased, but these grains have become unable to be utilized as livestock feed due to the appreciation of the yen value, hence the excess is now disposed of as wastes, causing an expense increase. Therefore, these will be incinerated together with the hydrated sludge. Because dehydrating the grains will produce fluids with high BOD value, the grains are to be burned as they are, and for this reason, a fluidized bed furnace was introduced. Effects of the incineration to the environment were surveyed, and it was confirmed that the parameters agreed with the Fukuoka Municipality can be maintained. The operation was begun in June 1989. As a result of the measures applied, an annual monetary saving of about 100 million yen can be expected out of the capital investment of 350 million yen, with the return of investment estimated to be made in about four years. 21 figs., 9 tabs.

  8. Energy concentration and phosphorus digestibility in yeast products produced from the ethanol industry, and in brewers' yeast, fish meal, and soybean meal fed to growing pigs.

    Science.gov (United States)

    Kim, B G; Liu, Y; Stein, H H

    2014-12-01

    Two experiments were conducted to determine the DE, ME, and standardized total tract digestibility (STTD) of P in 2 novel sources of yeast (C-yeast and S-yeast) and in brewers' yeast, fish meal, and soybean meal fed to growing pigs. The 2 new sources of yeast are coproducts from the dry-grind ethanol industry. The concentrations of DM, GE, and P were 94.8%, 5,103 kcal/kg, and 1.07% in C-yeast; 94.4%, 4,926 kcal/kg, and 2.01% in S-yeast; 93.6%, 4,524 kcal/kg, and 1.40% in brewers' yeast; 91.4%, 4,461 kcal/kg, and 3.26% in fish meal; and 87.7%, 4,136 kcal/kg, and 0.70% in soybean meal, respectively. The DE and ME in each of the ingredients were determined using 42 growing barrows (28.9±2.18 kg BW). A corn-based basal diet and 5 diets containing corn and 24% to 40% of each test ingredient were formulated. The total collection method was used to collect feces and urine, and the difference procedure was used to calculate values for DE and ME in each ingredient. The concentrations of DE in corn, C-yeast, S-yeast, brewers' yeast, fish meal, and soybean meal were 4,004, 4,344, 4,537, 4,290, 4,544, and 4,362 kcal/kg DM (SEM=57), respectively, and the ME values were 3,879, 3,952, 4,255, 3,771, 4,224, and 4,007 kcal/kg DM (SEM=76), respectively. The ME in S-yeast and fish meal were greater (Pcorn and brewers' yeast, whereas the ME in C-yeast and soybean meal were not different from those of any of the other ingredients. The STTD of P in the 5 ingredients was determined using 42 barrows (28.3±7.21 kg BW) that were placed in metabolism cages. Five diets were formulated to contain each test ingredient as the sole source of P, and a P-free diet was used to estimate the basal endogenous loss of P. Feces were collected for 5 d using the marker to marker method after a 5-d adaptation period. The STTD of P in brewers' yeast (85.2%) was greater (Pcorn, fish meal, and soybean meal, and the STTD of P in the 2 yeast products is not different from the STTD of P in fish meal.

  9. Activated carbon from pyrolysis of brewer's spent grain: Production and adsorption properties

    OpenAIRE

    2014-01-01

    Brewer’s spent grain is a low cost residue generated by the brewing industry. Its chemical composition (high nitrogen content 4.35 wt.%, fibres, etc.) makes it very useful for the production of added value in situ nitrogenised activated carbon. The composition of brewer’s spent grain revealed high amounts of cellulose (20.8 wt.%), hemicellulose (48.78 wt.%) and lignin (11.3 wt.%). The fat, ethanol extractives and ash accounted for 8.17 wt.%, 4.7 wt.% and 3.2 wt.%, respectively. Different ...

  10. Frequency of wet brewers grains supplementation during late gestation of beef cows and its effects on offspring postnatal growth and immunity.

    Science.gov (United States)

    Moriel, P; Artioli, L F A; Piccolo, M B; Marques, R S; Poore, M H; Cooke, R F

    2016-06-01

    Our objectives were to evaluate postnatal growth and measurements of innate and humoral immunity of beef calves born to dams fed wet brewers grains (WBG) daily or 3 times weekly during late gestation. On d 0 (approximately 60 d before calving), 28 multiparous, spring-calving Angus cows (BW = 578 ± 19 kg; age = 4.7 ± 0.65 yr; BCS = 7.0 ± 0.18) were stratified by sire, age, BW, and BCS and then randomly allocated into 1 of 14 drylot pens (2 cows/pen; 18 by 3 m; 27 m/cow). Cows were offered ground tall fescue hay ad libitum and received similar weekly WBG supplementation (DMI = 0.5% of BW multiplied by 7 d). Treatments were randomly assigned to pens (7 pens/treatment) and consisted of cows receiving WBG supplementation daily (S7; weekly DMI of WBG divided by 7 d) or 3 times weekly (S3; weekly DMI of WBG divided by 3 d; Mondays, Wednesdays, and Fridays) from d 0 until calving. Cow-calf pairs were managed as a single group on tall fescue pastures from calving to weaning (d 226). Calves were immediately submitted to a preconditioning period from d 226 to 266 and vaccinated against infectious bovine rhinotracheitis, bovine viral diarrhea virus, , and on d 231 and 245. Decreasing the frequency of WBG supplementation did not impact ( ≥ 0.21) precalving intake of total DM, CP, and TDN; BW and BCS change; overall plasma cortisol concentrations; and postcalving growth and pregnancy rate of cows. Overall plasma concentrations of glucose and insulin did not differ ( ≥ 0.28) between S3 and S7 cows, whereas S3 cows had greater ( = 0.002) plasma glucose concentrations and tended ( = 0.06) to have greater plasma insulin concentrations on days they were not fed WBG vs. days of WBG supplementation. Calf plasma concentrations of haptoglobin and cortisol at birth but not serum IgG ( = 0.63) tended ( = 0.10) to be greater for S3 vs. S7 calves. However, additional calf growth and immunity variables obtained during pre- and postweaning phases did not differ between S3 and S7 calves

  11. Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration.

    Science.gov (United States)

    Wang, F; Nishino, N

    2008-06-01

    Wet brewers grains and soybean curd residue were stored in laboratory-scale silos without (BG and SC silages, respectively) or with other ingredients as total mixed rations (BGT and SCT silages, respectively). Silages were opened after 14 and 56 d, and microbial counts, fermentation products, and aerobic stability were determined. Denaturing gradient gel electrophoresis was carried out to examine bacterial communities, and several bacteria that appeared to be involved in fermentation were identified. Lactic acid content was greater in SCT than in BGT silage, but lower in SC than in BG silage. Ethanol content was greater in BG than in SC regardless of silage type. Aerobic deterioration occurred promptly in ensiling materials (nonensiled by-products and total mixed ration mixtures) and in silages stored alone; however, SCT and BGT silages resisted deterioration and no heating was found for more than 5.5 d regardless of storage period. Silages were stable even with high yeast populations at silo opening, whereas prolonged ensiling decreased yeast counts in the 2 total mixed ration silages. The denaturing gradient gel electrophoresis profiles appeared similar between SCT and BGT silages but not between SC and BG silages. Weissella spp. and Lactobacillus brevis were common in aerobically stable SCT and BGT silages, and Lactobacillus buchneri was detected only in BGT silage. Both L. brevis and L. buchneri were found in silage but not in ensiling materials. Several other lactic acid bacteria were also identified in SCT and BGT silages, but did not appear to be related to fermentation and aerobic stability.

  12. The Content in Bioactive Compounds of Different Brewers' Spent Grain Aqueous Extracts

    Directory of Open Access Journals (Sweden)

    Anca FARCAS

    2016-11-01

    Full Text Available In the last decade by-products of food and beverage processing have attracted much attention due to their functionality and potential as food ingredients. Brewers’ spent grain is the major by-product of the brewing industry representing a valuable source of bioactive ingredients. The aim of this study was to assess the effect of extraction time and temperature on the efficiency of water as solvent for the extraction of bioactive compounds from brewers’ spent grain (BSG. In terms of extraction efficiency, the results from polyphenols, flavonoids and antioxidant activity, showed that the best extraction parameters for aqueous extracts are 90⁰C and 60 minutes. In comparison with the control, the best extraction method generated 87% of the phenolics and 43.46% of the flavonoids obtained by a methanol extraction. The preliminary results for the aqueous extracts showed that water can be used as extraction solvent, but a higher extraction time and temperature are needed in order to have a content in bioactive compounds similar to that of methanolic extracts. The obtained values for polyphenols, flavonoids and antioxidant activity, emphasize the importance and the opportunities of the reuse of this agro-industrial waste.

  13. Effects of Replacing Pork Back Fat with Brewer's Spent Grain Dietary Fiber on Quality Characteristics of Reduced-fat Chicken Sausages

    OpenAIRE

    Choi, Min-Sung; Choi, Yun-Sang; Kim, Hyun-Wook; Hwang, Ko-Eun; Song, Dong-Heon; n Lee, Soo-Yeo; Kim, Cheon-Jei

    2014-01-01

    The effects of replacing pork back fat with brewer's spent grain (BSG) pre-emulsion for physicochemical, textural properties, and sensory evaluations of reduced-fat chicken sausages are evaluated. Control was prepared with 15% pork back fat, and three reduced-fat chicken sausages were formulated with the replacement of 20, 25, and 30% pork back fat with BSG pre-emulsion. The pH level of reduced-fat sausages formulated with BSG pre-emulsion is lower than that of the control (p

  14. Reproductive performance of reindeer fed all-grain and hay-grain rations

    Directory of Open Access Journals (Sweden)

    J. M. Blanchard

    1986-06-01

    Full Text Available Reproductive performance of grain-fed reindeer {Rangifer tarandus was evaluated over a 2-year period. Groups of pregnant reindeer were fed one of three rations, (1 100% whole-grain barley, (2 98.9% whole-grain barley and 1.2% mineral and trace element supplement, and (3 70% whole-grain barley and 30% finely-chopped bluegrass hay. Reindeer fed unsupplemented whole-barley failed to produce a single live calf. The addition of mineral and trace element supplement to the ration did not result in any significant improvement in reproductive performance. Eighty-five percent of the reindeer consuming unsupplemented and supplemented all-barley rations became pregnant; however, 76% of the pregnancies resulted in stillborn calves. One-hundred percent of the cows maintained on the grain/hay ration produced live calves. We speculate that reproductive failure in reindeer cows maintained on all-grain rations is most likely a result of a diet induced disfunction in maternal rumen and/or carbohydrate metabolism rather than a micro-nutrient deficiency. More research is neeeded to determine which metabolic pathways are affected.

  15. Enhancement of biomass and fermentation activity of surplus brewers' yeast in a fed-batch process

    Energy Technology Data Exchange (ETDEWEB)

    Strel, B. (Ministry of Science and Technology, Ljubljana (Slovenia). Industrial Property Office); Grba, S.; Maric, V. (Zagreb Univ. (Croatia). Faculty of Food Technology and Biotechnology)

    1993-04-01

    The growth of surplus brewers' yeast in a fedbatch process was studied with the aim of increasing the fermentation activity of the yeast cells and of optimizing the growth conditions: 20 h cultivation at 30deg C and pH 5.0-5.5 using beet molasses as substrate, with a regulated feeding rate, showed satisfactory results. Under the chosen conditions, the final amount of biomass increased more than fivefold, achieving a specific growth rate of 0.1 h[sup -1] and substrate yield coefficient of 0.54 g.g[sup -1]. The increase in fermentation activity of yeast cells during cultivation correlated very well with the concentration of reduced glutathione, which increased from 1.2 to 2.7 mg.g[sup -1] (dry matter). At the same time the fermentation activity increased fivefold, which related to the nitrogen content of the yeast cells. Ethanol formation throughout the cultivation did not exceed 0.5 g.l[sup -1]. (orig.).

  16. The hydrolysis of protein of brewer's spent grains with neutrase%中性蛋白酶水解啤酒糟中蛋白质的研究

    Institute of Scientific and Technical Information of China (English)

    蔡俊; 邱雁临

    2001-01-01

    以啤酒厂主要副产物啤酒糟为主要的原料,利用中性蛋白酶对其蛋白质进行水解。研究了酶解的 pH温度、固液比、时间和加酶量对水解程度的影响,确定了最佳的影响因素。%The brewer's spent grains(BSG),the main byproduct of brewery,was hydrolyzed with neutrase. The effects of PH temperature,the proportion of solid to liquid(s% ),time and enzyme concentration on the hydrolysis were studied.The best effective factors were determined.

  17. A review of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef

    Directory of Open Access Journals (Sweden)

    Nader Glenn A

    2010-03-01

    Full Text Available Abstract Growing consumer interest in grass-fed beef products has raised a number of questions with regard to the perceived differences in nutritional quality between grass-fed and grain-fed cattle. Research spanning three decades suggests that grass-based diets can significantly improve the fatty acid (FA composition and antioxidant content of beef, albeit with variable impacts on overall palatability. Grass-based diets have been shown to enhance total conjugated linoleic acid (CLA (C18:2 isomers, trans vaccenic acid (TVA (C18:1 t11, a precursor to CLA, and omega-3 (n-3 FAs on a g/g fat basis. While the overall concentration of total SFAs is not different between feeding regimens, grass-finished beef tends toward a higher proportion of cholesterol neutral stearic FA (C18:0, and less cholesterol-elevating SFAs such as myristic (C14:0 and palmitic (C16:0 FAs. Several studies suggest that grass-based diets elevate precursors for Vitamin A and E, as well as cancer fighting antioxidants such as glutathione (GT and superoxide dismutase (SOD activity as compared to grain-fed contemporaries. Fat conscious consumers will also prefer the overall lower fat content of a grass-fed beef product. However, consumers should be aware that the differences in FA content will also give grass-fed beef a distinct grass flavor and unique cooking qualities that should be considered when making the transition from grain-fed beef. In addition, the fat from grass-finished beef may have a yellowish appearance from the elevated carotenoid content (precursor to Vitamin A. It is also noted that grain-fed beef consumers may achieve similar intakes of both n-3 and CLA through the consumption of higher fat grain-fed portions.

  18. Discarded oranges and brewer's spent grains as promoting ingredients for microbial growth by submerged and solid state fermentation of agro-industrial waste mixtures.

    Science.gov (United States)

    Aggelopoulos, Theodoros; Bekatorou, Argyro; Pandey, Ashok; Kanellaki, Maria; Koutinas, Athanasios A

    2013-08-01

    The exploitation of various agro-industrial wastes for microbial cell mass production of Kluyveromyces marxianus, kefir, and Saccharomyces cerevisiae is reported in the present investigation. Specifically, the promotional effect of whole orange pulp on cell growth in mixtures consisting of cheese whey, molasses, and potato pulp in submerged fermentation processes was examined. A 2- to 3-fold increase of cell mass was observed in the presence of orange pulp. Likewise, the promotional effect of brewer's spent grains on cell growth in solid state fermentation of mixtures of whey, molasses, potato pulp, malt spent rootlets, and orange pulp was examined. The cell mass was increased by 3-fold for K. marxianus and 2-fold for S. cerevisiae in the presence of these substrates, proving their suitability for single-cell protein production without the need for extra nutrients. Cell growth kinetics were also studied by measurements of cell counts at various time intervals at different concentrations of added orange pulp. The protein content of the fermented substrates was increased substantially, indicating potential use of mixed agro-industrial wastes of negligible cost, as protein-enriched livestock feed, achieving at the same time creation of added value and waste minimization.

  19. Effects of Storage Duration and Temperature on the Chemical Composition, Microorganism Density, and In vitro Rumen Fermentation of Wet Brewers Grains.

    Science.gov (United States)

    Wang, B; Luo, Y; Myung, K H; Liu, J X

    2014-06-01

    This study aimed to investigate the effects of storage duration and temperature on the characteristics of wet brewers grains (WBG) as feeds for ruminant animals. Four storage temperatures (5°C, 15°C, 25°C, and 35°C) and four durations (0, 1, 2, and 3 d) were arranged in a 4×4 factorial design. Surface spoilage, chemical composition and microorganism density were analyzed. An in vitro gas test was also conducted to determine the pH, ammonia-nitrogen and volatile fatty acid (VFA) concentrations after 24 h incubation. Surface spoilage was apparent at higher temperatures such as 25°C and 35°C. Nutrients contents decreased concomitantly with prolonged storage times (pyeast and mold increased (p<0.05) with increasing storage times and temperatures. As storage temperature increased, gas production, in vitro disappearance of organic matter, pH, ammonia nitrogen and total VFA from the WBG in the rumen decreased (p<0.01). Our results indicate that lower storage temperature promotes longer beneficial use period. However, when storage temperature exceeds 35°C, WBG should be used within a day to prevent impairment of rumen fermentation in the subtropics such as Southeast China, where the temperature is typically above 35°C during summer.

  20. Effects of Replacing Pork Back Fat with Brewer's Spent Grain Dietary Fiber on Quality Characteristics of Reduced-fat Chicken Sausages.

    Science.gov (United States)

    Choi, Min-Sung; Choi, Yun-Sang; Kim, Hyun-Wook; Hwang, Ko-Eun; Song, Dong-Heon; N Lee, Soo-Yeo; Kim, Cheon-Jei

    2014-01-01

    The effects of replacing pork back fat with brewer's spent grain (BSG) pre-emulsion for physicochemical, textural properties, and sensory evaluations of reduced-fat chicken sausages are evaluated. Control was prepared with 15% pork back fat, and three reduced-fat chicken sausages were formulated with the replacement of 20, 25, and 30% pork back fat with BSG pre-emulsion. The pH level of reduced-fat sausages formulated with BSG pre-emulsion is lower than that of the control (pfat chicken sausages increase proportionally with increasing BSG pre-emulsion (pfat contents and energy values are decreased in reduced-fat chicken sausages (pfat chicken sausages (pfat and the addition of BSG pre-emulsion had no influence on the cohesiveness of the chicken sausage. And there is no significant difference in the overall acceptability among control, T1 (chicken sausage with 20% of BSG pre-emulsion, 10% of fat addition), and T2 (chicken sausage with 25% of BSG pre-emulsion, 5% of fat addition) (p>0.05). Therefore, our results indicate that BSG is effective dietary fiber source for manufacturing of reduced-fat meat product and suggest that 20-25% of BSG pre-emulsion is suitable for pork back fat in chicken sausages.

  1. Excretion and degradation of dietary fiber constituents in ileostomy subjects consuming a low fiber diet with and without brewer's spent grain.

    Science.gov (United States)

    Aman, P; Zhang, J X; Hallmans, G; Lundin, E

    1994-03-01

    Excretion of total dietary fiber and dietary fiber components was studied in 10 human subjects with ileostomies, consuming in a crossover design a low fiber diet (15 g total dietary fiber/d) or this diet supplemented with 62 g/d of brewer's spent grain. Food and excreta were collected and analyzed on d 2, 3 and 7 of each dietary period. No significant differences in excretion of total dietary fiber or dietary fiber components were found on the three days. Excreta from subjects consuming the low fiber diet contained significantly more total dietary fiber than was ingested (20%, P importance of endogenous and microbial material, contributing to the total dietary fiber value, in ileostomy effluents. Analysis of specific dietary fiber components showed that this increased excretion was mainly due to fucose, mannose, galactose and uronic acid residues. When the high-fiber diet was consumed, there was a significantly greater excretion of the same non-starch polysaccharide residues as for consumption of the low fiber diet, but with a simultaneous decrease in excretion of arabinose, xylose and glucose residues (12%, P < 0.01), which were the major fiber components in the diet.

  2. Process optimization of brewers' grains fermentation using photosynthetic bacteria to make fish feed%光合菌发酵啤酒糟制鱼饲料工艺优化

    Institute of Scientific and Technical Information of China (English)

    张健; 冯学愚; 刘小彬; 谢刚; 方勤; 罗辉

    2011-01-01

    In order to get high-quality fish feed from brewers' spent grains, an anaerobic fermentation installation was set up, and the brewers' grains as raw material were pretreated by anaerobic photosynthetic bacteria separated from drainage ditch of brewers' grains in a beer factory. The orthogonal experiments were conducted based on single factors to study the semisolid fermentation technique for anaerobic photosynthetic bacteria culture. The results showed that the satisfied fermentation effect was attained under the technical conditions of dry brewers' grains/water 1:9 (mass fraction), inoculation amount 9%, illumination intensity 1100 Lx, fermentation time 5 d, fermentation temperature 30 ℃, and material thickness 3 cm. After fermentation, the real protein concentration of brewers' grains increased from 17.0% to 41.6%, the cellulose concentration decreased from 15.3% to 7.0%, the crude fat concentration increased from 5.9% to 6.6%, the crude ash concentration increased from 3.8% to 4.1%, the total phosphorus concentration increased from 0.6% to 1.3%, and the water concentration of dried brewers' grains was 9.8%. All the six indices satisfied the China standard of Grass Carp's fingerling (SC/T 1024-2002). The results can provide references for making active photosynthetic bacteria fish feed from brewers' grains.%为把啤酒糟加工成优质鱼饲料,从啤酒厂啤酒糟排水沟中以厌氧法分离出一株光合菌为菌种,对原料啤酒糟进行预处理,自组装厌氧发酵装置,采用单因素与正交试验法,对啤酒糟半固态培养光合菌的条件进行了研究.结果表明,当料水比1∶9(干糟:水,g/g)、接种量9%、光照强度1 100Lx、发酵时间5d、发酵温度30℃、料层厚度3 cm时,发酵效果较好.发酵后啤酒干糟真蛋白质量分数可从17.0%上升至41.6%、粗纤维素从15.3%下降至7.0%、粗脂肪从5.9%上升至6.6%、粗灰分从3.8%上升至4.1%、总磷从0.6%上升至1.3

  3. Fatty acids in beef from grain- and grass-fed cattle: the unique South ...

    African Journals Online (AJOL)

    2015-09-10

    Sep 10, 2015 ... Keywords: grain fed, grass fed, cattle, fatty acids, red meat. Fatty acids in beef ... of high-fat, low-carbohydrate diets.5. When dietary ..... fat-soluble vitamins. They also play ..... of South African dairy and beef cattle. SAJAS. 2013 ...

  4. Growth performance and resistance to Streptococcus iniae of juvenile Nile tilapia (Oreochromis niloticus) fed diets supplemented with GroBiotic - A and Brewtech Dried Brewers Yeast

    Science.gov (United States)

    This study was conducted to evaluate the effect of dietary levels of Brewtech® dried brewers yeast (BY) and GroBiotic®-A (GB) on growth performance, proximate body composition, immune response and resistance of juvenile Nile tilapia to Streptococcus iniae challenge. A practical basal (control) diet ...

  5. Effects of Brewers Spent Grains-typed Bio-organic Fertilizer on Cowpea Photosynthesis Characteristics%啤酒糟型生物有机肥对豇豆光合特性的影响

    Institute of Scientific and Technical Information of China (English)

    喻夜兰; 荣湘民; 刘强; 谢桂先; 曾晶

    2011-01-01

    [目的]研究在豇豆生长过程中施用啤酒糟型生物有机肥对豇豆光合特性的影响.[方法]采用田间小区试验,共设4个处理,以自然堆肥+化肥处理、纯化肥处理作为对照,比较啤酒糟型生物有机肥和双效活性精致有机肥2种肥料对豇豆功能叶光合特性的影响.[结果]在开花结英期,啤酒糟型生物有机肥处理豇豆功能叶净光合速率、胞间CO2浓度、叶绿素含量最高.[结论]啤酒糟型生物有机肥能明显促进豇豆中后期功能叶净光合速率,提高叶绿素含量,但对功能叶气孔导度影响不大.%[Objective] The effects of brewer's spent grains-typed bio-organic fertilizer on cowpea photosynthesis characteristics were studied. [ Method] Field plot experiments were conducted which had four treatments including natural compost, pure chemical fertilizer, brewers spent grains-typed bio-organic fertilizer, double effect exquisite bio-organic fertilizer. [Result] Net photosynthetic rate, intercellular CO2 concentra tion and content of chlorophyll of cowpea functional leaves were uppermost during flower buds. [ Conclusion ] Brewers spent grains-typed bio organic fertilizer could increase net photosynthetic rate in later stage of cowpea growth, improve the content of chlorophyll of functional leaves, decrease transpiration rate, and it had little effect on stomata conductance of functional leaves.

  6. Cellulase Production from Brewer's Spent Grain by Solid-State Fermentation with Aspergillus Niger 3.316%黑曲霉3.316固态发酵啤酒糟生产纤维素酶

    Institute of Scientific and Technical Information of China (English)

    杜晓梅; 付国家; 郭庆杰; 王许云

    2012-01-01

    以啤酒糟为主要原料,采用黑曲霉3.316(Aspergillus niger 3.316)固态发酵生产纤维素酶,对培养基组成和培养条件进行优化.结果表明:啤酒糟和麸皮的质量比为8∶2、水料质量比为1.5∶1、硫酸铵质量分数为3%、发酵时间为4d时,所产纤维素酶活性最大,滤纸酶活性(FPA)和羧甲基纤维素酶活性(CMCA)分别达72.6118和692.1700nkat.%Cellulase production was carried out by Solid-State Fermentation (SSF) with Aspergillus niger 3. 316 using brewer's spent grain as the main material. The SSF conditions were studied and optimized. It indicated that the optimum conditions were as follows: the ratio of brewer's spent grain and wheat bran was 8 :2, the ratio of water to material was 1. 5 : 1. the content of the ammonium sulfate was 3%, and the fermentation time was 4 d, respectively. Under the above-mentioned conditions, the filter paper activity (FPA) and carboxymethyl cellulase activity (CMCA) can reach 72.611 8and 692. 170 0 nkat, respectively.

  7. 利用啤酒糟制备高品质饲料蛋白%Study on the preparation of high-quality feedstuffs protein by brewer's spent grain

    Institute of Scientific and Technical Information of China (English)

    蔡国林; 张麟; 陆健

    2015-01-01

    通过刀片粉碎和筛分技术对啤酒糟进行预处理,对筛下物进行球磨处理、固态酶解和有益微生物发酵,可以显著提高啤酒糟的饲用品质,制备高品质饲料蛋白.啤酒糟经刀片粉碎后过60目筛,筛下物粗蛋白含量达到38.8%,粗纤维含量降低到1.89%,实现蛋白-纤维的初步富集分离.对筛下物球磨后,经纤维素酶、木聚糖酶和蛋白酶混合固态酶解和产朊假丝酵母JX-09固态发酵之后,其粗蛋白含量达到45.17%,比啤酒糟原样提高了59.6%,多肽含量从0.68%提高到5.21%,蛋白体外消化率从53.7%提高到61.8%,水解氨基酸总量达到42.18%,比啤酒糟原样提高了60%,必需氨基酸总量达到17.3%,比啤酒糟原样提高了60%,粗纤维含量达到1.66%,比啤酒糟原样降低了89%.%After blade grinding and sieving pretreatment,the partial of brewer' s spent grain with high level of protein was dealt with ball milling,solid enzymatic digestion and fermentation with beneficial microorganism.The feeding quality of brewer' s spent grain has been significantly improved,resulting in the production of feed protein with high-quality.The contents of crude protein and crude fiber in the undersize brewer's spent grain were 38.8% and 1.89%,respectively after blade grinding and filtration through a 60 mesh sieve.Consequently,the preliminary enrichment and separation of protein and fiber had been achieved.Moreover,the enzymatic efficiency of undersize brewer' s spent grain could be further improved after milling of fiber bundle by ball milling.After the hydrolysis by cellulase,xylanase,protease and solid fermentation with Candida utilis JX-09,the crude protein content of brewer's spent grain was increased up to 45.17%,which was 59.6% higher than that of original sample.Polypeptides content was increased from 0.68% to 5.21%,while the in vitro digestibility of proteins was increased from 53.7% to 61.8%.Total amino acids

  8. ADSORPTION ONTO BREWERS' SPENT GRAIN

    African Journals Online (AJOL)

    user

    water bodies are deleterious to human health and the environment [1]. The release of ... uses as animal feed and compost material, to reuse as food for people [8]. .... residual metal ion concentration was determined using atomic absorption ...

  9. Intake, performance and estimated methane production of Nellore steers fed soybean grain

    Directory of Open Access Journals (Sweden)

    Juliana Duarte Messana

    2014-12-01

    Full Text Available The objective of this study was to evaluate nutrient intake, performance, estimated methane production and carcass characteristics of steers fed diets containing three different levels of soybean grain: 0, 120 and 230 g/kg on the dry matter (DM. The study was conducted on eighteen castrated Nellore males with an average initial body weight (BW of 370±12 kg in a completely randomized design, with three treatments and six repetitions. Intake of dry matter (DMI, kg/day and crude protein tended to decrease linearly, and the intake of ether extract increased linearly in response to the soybean grain levels in the diet. Levels of soybean grain in the diet did not affect DMI (g/kg of BW, final average weight, average daily weight gain, feed efficiency and estimated methane production. Animals fed diets with different levels of soybean grain were not different with respect to carcass yield, loin eye area, subcutaneous fat thickness, or bone, muscle and fat ratios. Carcass characteristics and meat quality were also not significantly different. Replacement of soybean meal by soybean grain in the diet alters the intake of crude protein and ether extract but does not affect performance, estimates of methane production, or carcass characteristics of feedlot Nellore.

  10. Feeding slowly fermentable grains has the potential to ameliorate heat stress in grain-fed wethers.

    Science.gov (United States)

    Gonzalez-Rivas, P A; DiGiacomo, K; Russo, V M; Leury, B J; Cottrell, J J; Dunshea, F R

    2016-07-01

    During heat stress (HS), livestock reduce metabolic heat production by lowering activity and feed intake. Because this has obvious consequences for productivity, the aim of these experiments was to investigate nutritional methods for reducing digestive metabolic heat production, thereby allowing livestock more opportunity to dissipate excess heat. In the first experiment, the fermentation rates of corn and wheat grains were compared in an in vitro gas production system containing buffered rumen fluid. This experiment showed that corn had a slower (-15%; skin temperature (LFT and RFT, respectively) and blood acid-base balance. Rectal temperature, RR, LFT, and RFT were elevated ( impact of high environmental heat loads in sheep.

  11. Studies on the Production of Protein Feed through Liquid and Solid Co-fermentation from Dried Brewer's Grain%啤酒糟液固态混合发酵生产蛋白饲料的研究

    Institute of Scientific and Technical Information of China (English)

    赵启美; 何佳; 李西波

    2001-01-01

    The method of producing protein feed from dried brewer's grain through liquid and solid co-fermentation by many strain was studied. The results showed that the crude protein content in feed could be increased from 25.5% to 37.8%, the crude fiber content in feed could be declined from 16.3% to 9.4%, and also many active digestive enzymes could be contained in feed.%研究了以啤酒糟为主要原料,经多菌种液固态混合发酵生产蛋白饲料的工艺,结果显示:发酵料蛋白含量从啤酒糟的25.5%提高到37.8%,粗纤维由16.3%下降至9.4%,且含有多种消化酶。

  12. Performance and carcass traits of lambs fed diets containing different cereal grains

    Directory of Open Access Journals (Sweden)

    Marília Pastro Vidal

    2016-06-01

    Full Text Available The objective of this study was to evaluate the performance, apparent digestibility, and carcass and meat traits of lambs fed high-concentrate diets (92.65% containing different cereal grains (ground corn, whole grain corn, whole grain sorghum, or replacement of 40% whole grain corn with whole grain millet. Twenty-four feedlot intact lambs of undefined breed, with a body weight of 22.35 ± 3,.71 kg, kept in individual pens for 56 days were allocated in a completely randomized design. Dry matter intake expressed as a percentage of body weight was higher (P=0.0046 for the diets containing sorghum and ground corn (3.73% and 3.39%, respectively compared to the diets containing millet and whole grain corn (3.23% and 3.13%, respectively. Feed efficiency was higher (P=0.014 for whole grain corn and millet (0.293 and 0.269, respectively and lower for ground corn and sorghum (0.247 and 0.226, respectively. The apparent digestibility of organic matter, dry matter and crude protein was lower for the sorghum diet (P=0.0003. The carcass traits, meat cuts traits or objective longissimus muscle tenderness were not influenced by the diets offered to the animals. Meat color (P<0.0001 and luminosity (P=0.0063 were improved with inclusion of whole grains. The use of whole corn and millet grains is recommended for the feeding of feedlot lambs considering their nutritional benefits, feed efficiency, and improved carcass traits and meat quality attributes.

  13. FEDS

    DEFF Research Database (Denmark)

    Venable, John; Pries-Heje, Jan; Baskerville, Richard

    2016-01-01

    to enable Design Science Researchers to effectively design and incorporate evaluation activities into a DSR project that can achieve DSR goals and objectives. To address this research gap, this research paper develops, explicates, and provides evidence for the utility of a Framework for Evaluation in Design...... Science (FEDS) together with a process to guide design science researchers in developing a strategy for evaluating the artefacts they develop within a DSR project. A FEDS strategy considers why, when, how, and what to evaluate. FEDS includes a two-dimensional characterisation of DSR evaluation episodes...... on an actual DSR project....

  14. Phosphorus Utilization and Characterization of Excreta From Swine Fed Diets Containing A Variety of Cereal Grains Balanced For Total Phosphorus

    Science.gov (United States)

    Intrinsic phytase in swine feeds may alter phytate utilization and solubility of excreted phosphorus. Therefore, the objective of this experiment was to quantify changes in fecal phosphorus composition from swine fed various cereal grains with a range of phytate concentrations and endogenous phytase...

  15. FEDS

    DEFF Research Database (Denmark)

    Pries-Heje, Jan; Venable, John; Baskerville, Richard

    2016-01-01

    Evaluation of design artefacts and design theories is a key activity in Design Science Research (DSR), as it provides feedback for further development and (if done correctly) assures the rigour of the research. However, the extant DSR literature provides insufficient guidance on evaluation...... to enable Design Science Researchers to effectively design and incorporate evaluation activities into a DSR project that can achieve DSR goals and objectives. To address this research gap, this research paper develops, explicates, and provides evidence for the utility of a Framework for Evaluation in Design...... Science (FEDS) together with a process to guide design science researchers in developing a strategy for evaluating the artefacts they develop within a DSR project. A FEDS strategy considers why, when, how, and what to evaluate. FEDS includes a two-dimensional characterisation of DSR evaluation episodes...

  16. Effects of monensin supplementation on ruminal metabolism of feedlot cattle fed diets containing dried distillers grains.

    Science.gov (United States)

    Felix, T L; Pyatt, N A; Loerch, S C

    2012-11-01

    Two experiments were conducted to evaluate the effects of monensin and dried distillers grains with solubles (DDGS) on ruminal metabolism in 8 fistulated steers. In Exp. 1, treatments were (DM basis): 1) 0 mg monensin/kg diet DM, 2) 22 mg monensin/kg diet DM, 3) 33 mg monensin/kg diet DM, and 4) 44 mg monensin/kg diet DM. The remainder of the diet was 10% corn silage, 60% DDGS, 10% corn, and 20% mineral supplement that used ground corn as the carrier. There was no effect (P > 0.80) of dietary monensin inclusion on DMI. Increasing dietary monensin did not affect (P > 0.05) ruminal VFA concentrations or lactic acid concentrations. There was no effect (P > 0.15) of increasing dietary monensin concentration on ruminal hydrogen sulfide gas (H(2)S) and liquid sulfide (S(2-)) concentrations, or ruminal pH. In Exp. 2, treatments were arranged in a 2 × 2 factorial and contained (DM basis): 1) 0 mg monensin/kg diet DM + 25% DDGS inclusion, 2) 0 mg monensin/kg diet DM + 60% DDGS inclusion, 3) 44 mg monensin/kg diet DM + 25% DDGS inclusion, and 4) 44 mg monensin/kg diet DM + 60% DDGS inclusion. The remainder of the diet was 15% corn silage, corn, and 20% mineral supplement that used ground corn as a carrier. With 60% dietary DDGS inclusion, DMI decreased (P diet, 0 h postfeeding acetate concentration was decreased compared with when 60% DDGS was fed (P diets, regardless of monensin inclusion. This increase in propionate concentrations contributed to the increase (P = 0.03) in total VFA concentrations at 3 h postfeeding when 60% DDGS diets were fed. There was no interaction detected (P > 0.05) for H(2)S or S(2-) concentrations in Exp. 2. Feeding 60% DDGS diets increased mean H(2)S by 71% when compared with feeding 25% DDGS diets. Similar to the response observed for H(2)S, feeding 60% DDGS diets increased mean S(2-) by 64% when compared with feeding 25% DDGS diets. Although these studies did not show beneficial effects of monensin supplementation on ruminal pH, VFA, or H(2)S

  17. Techniques Optimization of a Feed Probiotic by Mixed Culture Fermentation on the Brewer's Grain%多菌固态发酵啤酒糟生产饲用微生态制剂工艺的优化研究

    Institute of Scientific and Technical Information of China (English)

    黄兴国; 杨承剑; 贺建华; 向平安; 黄璜

    2011-01-01

    In order to optimize brewers' grain fermentation technics for probiotics, the effects of 13 factors, such as urea, ammonium sulfate et al, on the total number of beneficial microbe in the probiotics, were studied by Plackett -Burman design and response surface methodology. The result showed that there were significantly relation among the total number of probiotics, urea, ammonium sulfate, fermentation temperature. The max predicted number of probiotics was 9.85 x 108CFU/g and the practical number was 9.73×108CFU/g when the urea concentration was 2.87%, ammonia sulfate concentration was 1.94%, fermentation temperature was 31.84℃.%为了优化多菌固态发酵啤酒糟制备饲用微生态制剂的工艺,应用Plackett-Burman设计及响应面分析法研究了尿素、硫酸铵、发酵温度等13个因素对微生态制剂中有益菌总数的影响.结果表明:尿素、硫酸铵、发酵温度与有益菌的总数存在显著的相关性.当尿素添加比例为2.87%、硫酸氨添加比例为1.94%,发酵温度为31.84℃时,有益茵活菌总数达到理论最大值9.85×108CFU/g,实际检测值为9.73×108CFU/g.

  18. Volatile organic compound flux from manure of cattle fed diets differing in grain processing method and co-product inclusion

    Science.gov (United States)

    Hales, Kristin; Parker, David B.; Cole, N. Andy

    2015-01-01

    Odor emissions from livestock production have become increasingly important in the past decade. Odors derived from animal feeding operations are caused by odorous VOC emitted from the mixture of feces and urine, as well as feed and silage which may be experiencing microbial fermentation. Distillers grains are a by-product of corn grain fermentation used to produce fuel ethanol, and this industry has grown rapidly throughout the U.S. in past years. Therefore, the use of wet distillers grains with solubles (WDGS) in feedlot cattle diets has also increased. The objective of this research was to determine specific VOC emissions from feces and urine or a mixture of both, from cattle fed steam flaked or dry-rolled corn (DRC)-based diets containing either 0% or 30% WDGS. Flux of dimethyl trisulfide was greater from feces of cattle fed DRC than steam-flaked corn (SFC) diets. No other differences in flux from feces were detected across dietary treatments for phenol, 4-methylphenol, indole, skatole, dimethyl disulfide, and flux of volatile fatty acids (VFA) such as acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids (P > 0.15). Flux of skatole, acetic acid, and valeric acid from urine was greater for cattle fed SFC than DRC diets (P acetic acid and heptanoic acid from urine was greater when cattle were fed diets containing 0% WDGS than 30% WDGS (P < 0.05). When combining urine and feces in the ratio in which they were excreted from the animal, flux of propionic acid was greater when cattle were fed DRC vs. SFC diets (P = 0.05). Based on these results, the majority of the VOC, VFA, and odor flux from cattle feeding operations is from the urine. Therefore, dietary strategies to reduce odor from cattle feeding facilities should primarily focus on reducing excretion of odorous compounds in the urine.

  19. Brewer's sparrow (Spizella breweri)

    Science.gov (United States)

    Dawson, D.K.; Armbruster, J.S.

    1983-01-01

    Many of the known recoverable coal reserves in the Western United States occur within the breeding and wintering range of the Brewer's sparrow. Although the species is common throughout much of its breeding range, local and regional populations may be adversely affected due to mining-related disturbances and habitat loss. The species may serve as a valuable indicator of the impacts of surface-mining activities because it is restricted primarily to the sagebrush type during the breeding season and is known to be sensitive to habitat change. Recommendations for mitigating impacts focus on minimizing disturbance during the breeding season and re-establishing preferred native vegetation on mined sites. Monitoring populations on and adjacent to mine sites will give a better understanding of the effects of disturbance and the success of mitigation efforts.

  20. 机械预处理对酶解啤酒糟提取阿魏酰低聚糖的影响%Effect of mechanical pretreatment on extraction of feruloyl oligosaccharides from brewer's spent grain by enzymatic hydrolysis

    Institute of Scientific and Technical Information of China (English)

    张秋培; 尤梦竹; 蔡国林; 曹钰

    2015-01-01

    Mechanical pretreatment of brewer's spent grain (BSG) can change its tightness and the particle size,thus affecting enzymatic hydrolysis efficiency.With the increase of degree of crushing,enzymatic efficiency increase.The yield of feruloyl oligosaccharides (FOs) from untreated BSG by xylanase and cellulase were 31.89% and 33.41%,respectively.And after the synergy of xylanase and cellulase,the yield of FOs increased to 47.01%.The yield of FOs from mechanical treated BSG hydrolyzed by xylanase and cellulase were 39.49% and 50.36%,respectively.And after the synergy of xylanase and cellulase,the yield of FOs increased to 64.00%.The results indicated that pretreatment could improve the' efficiency of enzymatic hydrolysis,and the presence of cellulase and β-glucanase was beneficial to the effect of xylanase,thus improved the extraction efficiency of FOs from BSG and initially realized the high-value utilization of BSG.%啤酒糟经预处理后可以改变其紧密度以及粒径大小,从而影响酶解效率,随着粉碎程度的增加,酶解效率增加,其中,啤酒糟原样经木聚糖酶和纤维素酶水解后,阿魏酰低聚糖(FOs)的得率分别为31.89%和33.41%,木聚糖酶和纤维素酶协同作用后,FOs的得率为47.01%;刀片粉碎后球磨30 min的啤酒糟,经木聚糖酶和纤维素酶水解后,FOs的得率分别为39.49%和50.36%,而经木聚糖酶和纤维素酶协同作用后,FOs的得率增加到了64.00%.实验结果表明,预处理可以提高酶解效率,纤维素酶和β-葡聚糖酶的存在有利于木聚糖酶的作用,从而在一定程度上提高从啤酒糟中提取FOs的效率,初步实现啤酒糟的高值化利用.

  1. Lipopolysaccharide derived from the digestive tract provokes oxidative stress in the liver of dairy cows fed a high-grain diet.

    Science.gov (United States)

    Abaker, J A; Xu, T L; Jin, D; Chang, G J; Zhang, K; Shen, X Z

    2017-01-01

    The aims of this study were to measure oxidative stress parameters and to investigate the molecular mechanism triggered by grain-induced subacute ruminal acidosis in mid-lactation cows. Twelve Holstein-Friesian cows with an average weight of 455±28kg were divided into 2 groups and subjected to 2 diets over 18wk: either a low-grain (forage-to-concentrate ratio=6:4) or a high-grain (forage-to-concentrate ratio=4:6) diet based on dry matter. Being fed a long-term high-grain diet resulted in a significant decrease in rumen pH and a significant increase in ruminal lipopolysaccharide (LPS) at 4 h postfeeding in the morning. The increase was also observed in LPS concentrations in the portal vein, hepatic vein, and jugular vein blood plasma as well as reduced milk yield in a high-grain diet. Cows fed a high-grain diet had lower levels of catalase and glutathione peroxidase (GPx) activity and total antioxidant capacity than cows fed a low-grain diet; however, super oxide dismutase (SOD) activity and malondialdehyde (MDA) levels were higher in both the liver and the plasma of high-grain than in low-grain cows. Positive correlations were observed between plasma LPS versus hepatic MDA, plasma MDA, and hepatic SOD activity, whereas hepatic GPx and plasma GPx were negatively correlated with plasma LPS. The relative mRNA abundances of GPX1 and CAT were significantly lower in the liver of cows fed a high-grain diet than those fed a low-grain diet, whereas SOD1 was significantly higher in cows fed a high-grain diet than cows fed a low-grain diet. The expression levels of Nrf2, NQO1, MT1E, UGT1A1, MGST3, and MT1A were downregulated, whereas NF-kB was upregulated, in cows fed a high-grain diet. Furthermore, nuclear factor E2-related factor 2 (Nrf2) total protein and mRNA levels were significantly lower than in low-grains. Our results demonstrate the relationship between the translocated LPS and the suppression of cellular antioxidant defense capacity, which lead to increased

  2. Weight Gain and Feed Conversion Efficiency of Sansui Duck Fed with Brewer’s Grains%白酒糟饲喂三穗鸭的增重及饲料转化效果

    Institute of Scientific and Technical Information of China (English)

    方园; 夏先林; 方福平; 乔艳龙; 陶忠海

    2014-01-01

    To explore the rational utilization of brewer’s grains in Sansui Duck breeding,280 healthy Sansui ducks(21 days old)were selected and randomly divided into four groups to study the effects of different daily ration of brewer's grains on the weight gain and feed efficiency.Results:the daily gain of Sansui Duck fed with mixed feed(CK)was the highest(16.7 g),respectively higher than GroupⅠ(90%mixed feed+10% brewer's grains),GroupⅡ(85% mixed feed+15% brewer’s grains)and Group Ⅲ(80%mixed feed+20% brewer's grains)by 5.7%(P >0.05),21%(P 0.05)respectively;The energy conversion efficiency of Group Ⅱ was the highest(0.57),higher than CK,GroupⅠ and Group Ⅲ by 23.9%(P 0.05)separately;The protein efficiency ratio of CK was the highest(1.65),respectively higher than GroupⅠ,Group Ⅱ and Group Ⅲ by 13% (P < 0.05),46% (P < 0.05)and 42.2% (P < 0.05) respectively.It was optimum to replace mixed feed by 10% brewer's grains in 4~10 week age of Sansui duck,which had no obvious influence on the growth performance and feed conversion efficiency.%为白酒糟资源在三穗鸭养殖上的合理利用,选取21日龄健康三穗鸭280只,随机分为4组进行了不同白酒糟水平日粮对其增重及饲料转化效率的影响试验。结果表明:日增重以饲喂配合饲料(对照组)的最高,为16.7 g,分别比试验Ⅰ组(90%配合饲料+10%白酒糟生物饲料)、试验Ⅱ组(85%配合饲料+15%白酒糟生物饲料)、试验Ⅲ组(80%配合饲料+20%白酒糟生物饲料)高5.7%(P >0.05)、21%(P <0.05)和16%(P <0.05)。料重比以试验Ⅱ组最高,为5.5,分别比对照组、试验Ⅰ组、试验Ⅲ组高41%(P <0.05)、25%(P <0.05)和1.9%(P >0.05);代谢能增重效率以试验Ⅱ组最高,为0.57,分别比对照组、试验Ⅰ组、试验Ⅲ组高23.9%(P <0.05)、18.8%(P <0.05)和5.6%(P >0.05);

  3. Beef quality of calf-fed steers finished on varying levels of corn-based wet distillers grains plus solubles.

    Science.gov (United States)

    Mello, A S; Calkins, C R; Jenschke, B E; Carr, T P; Dugan, M E R; Erickson, G E

    2012-12-01

    Calf-fed crossbred steers (n = 94) were randomly allocated to 3 dietary treatments (0%, 15%, or 30% wet distillers grains plus solubles, WDGS; DM basis) and fed for 167 d to determine the effects on quality attributes of beef. At 48 h postmortem, marbling score, marbling texture, and marbling distribution were assessed by a USDA grader. After grading, one rib eye slice (longissimus thoracis) »7 mm thick was excised from each carcass, trimmed of subcutaneous fat, and analyzed for fatty acid profile and lipid content. At 7 d postmortem, 48 top blades (infraspinatus), strip loins (longissimus lumborum), and tenderloins (psoas major) (16 per treatment) were removed from shoulder clods and short loins and 2 steaks were obtained to measure mineral content, fatty acid profile (except strip loins), trained sensory analysis, objective color, and lipid oxidation. Finishing diet did not influence the content of total lipid (P = 0.19) or marbling, marbling texture, or marbling distribution (P = 0.46, 0.84, 0.40, respectively). Feeding WDGS created a linear increase (P 0.50), except a minimal effect on strip loin juiciness (5.32, 4.86, and 5.52 for 0%, 15%, and 30% WDGS, respectively; P = 0.02). Top blade and tenderloin steaks from cattle fed 30% WDGS were significantly less red (lower a* values) on d 3 of simulated retail display (P Feeding WDGS to calf-fed steers altered fatty acid profile, increased oxidation, and decreased color stability during retail display.

  4. Performance of Holstein cows fed sugarcane or corn silages of different grain textures

    Directory of Open Access Journals (Sweden)

    Corrêa Clóvis Eduardo Sidnei

    2003-01-01

    Full Text Available Corn cultivated in Brazil is predominantly of hard texture, and more propense to decreased starch digestibility under late harvesting situations than dent hybrids. This work tested the utilization of dent corn as a way of extending the ensilage period without reducing animal performance, and evaluated the potential of sugarcane as a forage for high-producing dairy cows. Nine lactating Holstein cows were allocated to three 3 ' 3 latin squares and were fed 200 g of forage neutral detergent fiber per kg of dry matter as either hard texture corn ensiled at the half milk line stage of maturity, soft texture corn ensiled at the black layer stage, or sugarcane. There were no detectable differences between corn hybrids with regard to milk yield (34.2 vs 34.6 kg d-1 and composition, dry matter intake (23.0 vs 23.2 kg d-1 and total tract apparent digestibility of nutrients. Sugarcane decreased feed intake (21.5 kg d-1 and milk yield (31.9 kg d-1. Organic matter digestibility, chewing activity and rumen pH did not differ among treatments. Sugarcane seems to be a viable option to feed groups of Holstein cows during lactation stages in which nutrient demand is not at a maximum. The performance of dairy cows fed dent corn ensiled at the black layer stage of maturity was similar to the performance of cows fed flint corn ensiled at the half milk line stage.

  5. Nutritional quality of eggs from hens fed distillers dried grains with solubles

    Science.gov (United States)

    A feeding trial was conducted with laying hens where either 10% or 20% regular-fat distiller’s dried grains with solubles (R-DDGS) or low-fat DDGS (L-DDGS) were incorporated into the feed. Production parameters and the effect of DDGS on egg nutritional quality, focusing on yolk lipids, were evaluate...

  6. Performance of broilers fed during 21 days on mash or pellet diets containing whole or ground pearl millet grain

    Directory of Open Access Journals (Sweden)

    TR Torres

    2013-12-01

    Full Text Available An experiment was carried out to evaluate the effect of the inclusion of 20% whole-grain or ground pearl millet (PM in mash and pelleted diets on the performance, carcass traits, and organ weights of broilers reared until 21 days of age. A randomized block experimental design in a 3 x 2 factorial arrangement (diets containing corn and soybean meal, whole-grain PM, or ground PM x mash or pelleted diets, with five replicates per treatment and 10 birds per experimental unit, was applied. Diets were analyzed for mean geometric diameter, geometric standard deviation, pellet hardness, and density. Broiler performance, carcass yield, and organ weights were evaluated. On day 21, one bird with the average weight of each experimental unit was sacrificed for carcass evaluation. It was concluded that both as whole-grain and ground PM can be added to the diet of broilers up to 21 days of age. The dietary inclusion of PM results in higher abdominal fat deposition. Broilers fed the pelleted diets presented lower feed intake, better feed conversion ratio, lower gizzard and heart percentages, and higher carcass weight.

  7. Effects of alternate-day feeding of dried distiller's grain plus solubles to forage-fed beef cows in mid- to late gestation.

    Science.gov (United States)

    Klein, S I; Steichen, P L; Islas, A; Goulart, R S; Gilbery, T C; Bauer, M L; Swanson, K C; Dahlen, C R

    2014-06-01

    Forty-six nonlactating beef cows were used to examine effects of dried distiller's grains plus solubles (DG) supplementation strategies to cows fed grass hay during mid- to late gestation on BW, ultrasound body composition characteristics, concentrations of serum NEFA and urea, feeding behavior, and calf birth weight. Cows were assigned to dietary treatments in a completely randomized design: 1) control, where hay was fed each day of the week (CON), 2) both hay and DG fed daily during the week (DG7), 3) hay fed daily but DG fed 3 d of the week (DG3), and 4) hay fed 4 d of the week alternating with DG fed on the remaining 3 d (DGA). Hay was offered ad libitum on days it was fed. The DG were fed at 0.40% of BW when offered daily and 0.93% of BW when offered 3 d per week (Monday, Wednesday, and Friday). Feed intake was monitored continuously over the 84-d feeding period. Hay intake and total DMI were reduced (P 0.05) were observed among treatments for change in BCS, intramuscular fat, rib fat, or rump fat from d 1 to 84. On a day when DG7, DG3, and DGA all received DG (Friday), DGA had reduced (P 0.05) were observed in calf birth weights among treatments. The alternate-day feeding strategy reduced hay and total intake, altered concentrations of serum urea and NEFA, and altered feeding behavior compared with other supplementation methods.

  8. Growth performance and total tract nutrient digestion for Holstein heifers limit-fed diets high in distillers grains with different forage particle size

    Science.gov (United States)

    This study evaluated dairy heifer growth performance and total tract nutrient digestion when fed diets high in dried distillers grains with solubles (DDGS) with different forage particle size. An 8-wk randomized complete block design study was conducted utilizing twenty-two Holstein heifers (123 ±...

  9. Alternating dietary fat sources for growing-finishing pigs fed dried distillers grains with solubles: II. Fresh belly and bacon quality characteristics.

    Science.gov (United States)

    Browne, N A; Apple, J K; Maxwell, C V; Yancey, J W; Johnson, T M; Galloway, D L; Bass, B E

    2013-03-01

    Crossbred pigs (n = 216) were used to test the effects of phase-feeding beef tallow (BT) and yellow grease (YGr) on fresh belly and bacon quality characteristics of growing-finishing swine fed dried distillers grains with solubles (DDGS). Pigs were blocked by initial BW (26.0 ± 5.3 kg) before allotment to pens (6 pigs/pen), and pens (6 pens/block) were assigned randomly to 1 of 6 dietary treatments: 1) corn-soybean meal-based grower and finisher diets formulated with 4.7% YGr fed during all 5 feeding phases (YG15); 2) corn-soybean meal-based diets formulated with 5.0% BT fed during all 5 phases (BT15); 3) diets containing 5.0% BT fed during the first 2 phases and diets with 4.7% YGr fed the last 3 phases (YG345); 4) diets formulated with 5.0% BT fed during first 3 phases and diets containing 4.7% YGr fed during the last 2 phases (YG45); 5) diets containing 4.7% YGr fed during the first 3 phases and diets with 5.0% BT fed during the last 2 feeding phases (BT45); or 6) diets formulated with 4.7% YGr fed during the first 2 phases and diets with 5.0% BT fed during the last 3 phases (BT345). All dietary treatments were formulated with 30% dried distillers grains with solubles (DDGS) during the first 3 phases, 15% DDGS in the fourth phase, and no DDGS during the last phase. Fresh belly quality data were collected on the left-side bellies, whereas bacon from the right-side bellies was prepared under commercial processing conditions. Additionally, USDA-certified No. 1 slices were collected for cooking characteristics and sensory panel evaluations. Bellies from the YG15-fed pigs were softer (P ≤ 0.05) than bellies from BT15-fed pigs; however, instrumentally measured belly firmness was not (P ≥ 0.06) different among treatments. Concentrations of palmitic, stearic, and oleic acids, as well as all SFA and all MUFA, were greater (P bacon (P ≥ 0.06), mechanical bacon tenderness (P ≥ 0.69), and bacon palatability attributes (P ≥ 0.55) were not affected by the dietary

  10. Emissions of greenhouse gases, ammonia, and hydrogen sulfide from pigs fed standard diets and diets supplemented with dried distillers grains with solubles.

    Science.gov (United States)

    Trabue, Steven; Kerr, Brian

    2014-07-01

    Swine producers are supplementing animal diets with increased levels of dried distillers grains with solubles (DDGS) to offset the cost of a standard corn-soybean meal (CSBM) diet. However, the environmental impact of these diets on emissions of greenhouse gases, ammonia (NH), and hydrogen sulfide (HS) is largely unknown. Twenty-four pigs (103.6 kg initial body weight) were fed a standard CSBM diet or a CSBM diet containing 35% DDGS for 42 d. Pigs were fed and their manure was collected twice daily over the 42-d trial. Pigs fed diets containing DDGS had reduced manure pH ( < 0.01), increased surface crust coverage ( < 0.01), increased manure dry matter content ( < 0.01), and increased manure C ( < 0.01), N ( < 0.01), and S ( < 0.01) contents. Animals fed DDGS diets also had significantly higher concentrations of total ammoniacal nitrogen ( < 0.01) and sulfide ( < 0.01) in their manure compared with animals fed CSBM diets. Manure emissions of NH ( < 0.01) and HS ( < 0.05) were significantly higher in animals fed the CSBM diet. There was no dietary treatment effect for methane or nitrous oxide emissions from manure. This study demonstrates that diets containing DDGS can significantly affect manure composition and potentially lower emissions of NH and HS.

  11. Effect of substituting brown rice for corn on lactation and digestion in dairy cows fed diets with a high proportion of grain.

    Science.gov (United States)

    Miyaji, M; Matsuyama, H; Hosoda, K

    2014-02-01

    The effects of the substitution of brown rice (Oryza sativa L.; BR) for corn (Zea mays L.) in ensiled total mixed ration (TMR) that had a high proportion of grain on feed intake, lactation performance, ruminal fermentation, digestion, and N utilization were evaluated. Nine multiparous Holstein cows (51 ± 9 d in milk) were used in a replicated 3 × 3 Latin square design with 3 dietary treatments: a diet containing 0, 20, or 40% steam-flaked BR and 40, 20, or 0% steam-flaked corn (dry matter basis). Cows were fed ad libitum an ensiled TMR consisting of 40.7% alfalfa silage, 11.8% grass silage, 7.1% soybean meal, and 40.0% steam-flaked grain (dry matter basis). The ensiled TMR was prepared by baling fresh TMR, and then sealed by a bale wrapper and stored outdoors at 5 to 30 °C for over 6 mo. Dry matter intake and milk yield were lower for cows fed 40% BR than for cows fed 40% corn. The ruminal pH and total volatile fatty acid concentrations were not affected by dietary treatment. The ruminal ammonia-N concentration decreased as the percentage of BR in the diets was elevated. The proportion of acetate decreased, and that of propionate and butyrate increased with the increasing levels of BR. Plasma urea-N concentrations was lower and glucose and insulin concentrations were higher for cows fed 40% BR than for cows fed 40% corn. The whole-tract apparent digestibility of dry matter, organic matter, and starch increased, and the digestibility of neutral detergent fiber and acid detergent fiber decreased with the increasing BR level in the diet, with no dietary effect on crude protein digestion. As a proportion of N intake, the urinary N excretion was lower and the retention of N was higher for cows fed 40% BR than for cows fed 40% corn, with no dietary effect observed on N secretion in milk and fecal N excretion. These results show that substituting BR for corn decreases urinary N losses and improves N utilization, but causes adverse effects on milk production when cows

  12. Growth performance, feeding behavior, and selected blood metabolites of Holstein dairy calves fed restricted amounts of milk: No interactions between sources of finely ground grain and forage provision.

    Science.gov (United States)

    Mirzaei, M; Khorvash, M; Ghorbani, G R; Kazemi-Bonchenari, M; Ghaffari, M H

    2017-02-01

    The objective of this study was to investigate the effects of grain sources and forage provision on growth performance, blood metabolites, and feeding behaviors of dairy calves. Sixty 3-d-old Holstein dairy calves (42.2 ± 2.5 kg of body weight) were used in a 2 × 3 factorial arrangement with the factors being grain sources (barley and corn) and forage provision (no forage, alfalfa hay, and corn silage). Individually housed calves were randomly assigned (n = 10 calves per treatment: 5 males and 5 females) to 6 treatments: (1) barley grain (BG) without forage supplement, (2) BG with alfalfa hay (AH) supplementation, (3) BG with corn silage (CS) supplementation, (4) corn grain (CG) without forage supplement, (5) CG with AH supplementation, and (6) CG with CS supplementation. All calves had ad libitum access to water and starter feed throughout the experiment. All calves were weaned on d 49 and remained in the study until d 63. Starter feed intake and average daily gain (ADG) was greater for calves fed barley than those fed corn during the preweaning and overall periods. Calves supplemented with CS had greater final body weight and postweaning as well as overall starter feed intake than AH and non-forage-supplemented calves. During the preweaning and overall periods, feeding of CS was found to increase ADG compared with feeding AH and nonforage diets. However, feed efficiency was not affected by dietary treatments. Calves supplemented with CS spent more time ruminating compared with AH and control groups; nonnutritive oral behaviors were the greatest in non-forage-supplemented calves. Regardless of the grain sources, the rumen pH value was greater for AH calves compared with CS and non-forage-supplemented calves. Blood concentration of BHB was greater for CS-supplemented calves compared with AH and non-forage-supplemented calves. Furthermore, body length and heart girth were greater for calves fed barley compared with those fed corn, and also in forage

  13. Performance of lactating dairy cows fed silage and grain from a maize hybrid with the cry1F trait versus its nonbiotech counterpart.

    Science.gov (United States)

    Faust, M; Smith, B; Rice, D; Owens, F; Hinds, M; Dana, G; Hunst, P

    2007-12-01

    Effects of feeding grain and maize silage from a non-Bt maize and a variety of Bt maize that contains cry1F (event TC1507, event DAS-Ø15Ø7-1), a gene that provides maize with insect resistance, on the health and performance of dairy cows were evaluated. In a crossover trial, 20 lactating Holstein cows were assigned to each of 2 dietary treatment groups and fed diets containing whole-plant maize silage plus maize grain from TC1507 or its near-isoline counterpart (control). Each period of the crossover trial lasted 28 d and was preceded by a 7-d adjustment period. To minimize variability due to stage of lactation, 2 blocks of 10 cows at 90 to 130 d of lactation at the start of the trial were used. Within each dietary treatment, 10 cows were from each of 2 genetic selection lines (high and average fat plus protein predicted transmitting ability). Diets were formulated to be isocaloric and isonitrogenous. Dry matter intake and daily production of milk, fat, protein, lactose, nonfat solids, and total solids did not differ between cows fed the TC1507 diet and cows fed the control diet. Furthermore, milk from cows in different dietary treatment groups did not differ in milk urea nitrogen concentration or somatic cell count. For milk fat percentage, a significant dietary treatment by genetic group interaction was detected although overall yield of milk and solids-corrected milk did not differ with diet. Physical measures of cow health including body weight, body condition score, temperature, pulse, and respiration rate were collected weekly; dietary treatment group means for these measures were not different. Blood chemistry and hematological analyses were conducted using blood samples collected from cows before the start of the trial and at the end of each period. Overall, the TC1507 and control groups did not differ in any of these indices of health status. Further, hematological profiles for cows in the dietary treatment groups were not different. In summary, no

  14. Evaluation of commercially available enzymes, probiotics, or yeast on apparent total-tract nutrient digestion and growth in nursery and finishing pigs fed diets containing corn dried distillers grains with solubles

    Science.gov (United States)

    The ability of enzymes, direct fed microbials, or yeast to enhance nutrient utilization or growth performance in nursery or finishing pigs fed diets containing increased levels of corn fiber from dried distillers grains with solubles (DDGS) is largely unknown. Ten commercially available feed additiv...

  15. Relationship of severity of subacute ruminal acidosis to rumen fermentation, chewing activities, sorting behavior, and milk production in lactating dairy cows fed a high-grain diet.

    Science.gov (United States)

    Gao, X; Oba, M

    2014-05-01

    The objectives of the current study were to evaluate the variation in severity of subacute ruminal acidosis (SARA) among lactating dairy cows fed a high-grain diet and to determine factors characterizing animals that are tolerant to high-grain diets. Sixteen ruminally cannulated late-lactating dairy cows (days in milk=282 ± 33.8; body weight=601 ± 75.9 kg) were fed a high-grain diet consisting of 35% forage and 65% concentrate mix. After 17 d of diet adaptation, chewing activities were monitored for a 24-h period and ruminal pH was measured every 30s for 72 h. Acidosis index, defined as the severity of SARA (area of pH acidosis index ranged from 0.0 to 10.9 pH · min/kg of DMI. Six cows with the lowest acidosis index (0.04 ± 0.61 pH · min/kg) and 4 with the highest acidosis index (7.67 ± 0.75 pH · min/kg) were classified as animals that were tolerant and susceptible to the high-grain diet, respectively. Total volatile fatty acid concentration and volatile fatty acid profile were not different between the groups. Susceptible animals sorted against long particles, whereas tolerant animals did not (sorting index=87.6 vs. 97.9, respectively). However, the tolerant cows had shorter total chewing time (35.8 vs. 45.1 min/kg of DMI). In addition, although DMI, milk yield, and milk component yields did not differ between the groups, milk urea nitrogen concentration was higher for tolerant cows compared with susceptible cows (12.8 vs. 8.6 mg/dL), which is possibly attributed to less organic matter fermentation in the rumen of tolerant cows. These results suggest that a substantial variation exists in the severity of SARA among lactating dairy cows fed the same high-grain diet, and that cows tolerant to the high-grain diet might be characterized by less sorting behavior but less chewing time, and higher milk urea nitrogen concentration. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  16. The effects of whole grains on nutrient digestibilities, growth performance, and cecal short-chain fatty acid concentrations in young chicks fed ground corn-soybean meal diets.

    Science.gov (United States)

    Biggs, P; Parsons, C M

    2009-09-01

    Five experiments were conducted to evaluate the effects of whole wheat, whole sorghum, or whole barley on nutrient digestibility, growth performance, and cecal short-chain fatty acid concentrations when supplemented primarily at the expense of corn in ground corn-soybean meal control diets. The first 4 experiments utilized New Hampshire x Columbian male chicks. In the first 2 experiments, feeding 5, 10, 15, or 20% whole wheat had no effect on growth performance at 21 d when compared with chicks fed the control diet. The third experiment tested 20, 35, and 50% whole wheat fed from 0 to 21 d of age and showed that a 50% whole wheat diet decreased (Pwhole sorghum reduced (Pwhole barley had similar weight gains to chicks fed a ground corn-soybean meal diet. The fifth experiment with commercial Ross x Ross male broiler chicks evaluated 10 and 20% whole sorghum or whole barley and 20 and 35% whole wheat. Growth at 21 d was unaffected by any dietary treatment. Feed efficiency was decreased (Pwhole wheat and improved (Pwhole barley. Feeding whole grains to chicks resulted in an increase in gizzard weight, even as early as 7 d, in all experiments. Chicks fed diets containing 10 to 20% whole wheat generally had increased MEn values at 3 to 4, 7, 14, and 21 d and also had increased amino acid digestibility at 21 d in one experiment. At 21 d, cecal pH and short-chain fatty acid concentrations in all experiments were unaffected by feeding whole grains to chicks. The results of this study indicated that feeding whole wheat, sorghum, or barley increased gizzard weight, and feeding 10 to 20% whole wheat may increase ME and amino acid digestibility.

  17. 27 CFR 28.225 - Removals of beer by brewer.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Removals of beer by brewer..., DEPARTMENT OF THE TREASURY LIQUORS EXPORTATION OF ALCOHOL Exportation of Beer With Benefit of Drawback Execution of Claims § 28.225 Removals of beer by brewer. Where a brewer removes taxpaid beer from...

  18. In situ identification and quantification of starch-hydrolyzing bacteria attached to barley and corn grain in the rumen of cows fed barley-based diets.

    Science.gov (United States)

    Xia, Yun; Kong, Yunhong; Seviour, Robert; Yang, Hee-Eun; Forster, Robert; Vasanthan, Thavaratnam; McAllister, Tim

    2015-08-01

    Cereal grains rich in starch are widely used to meet the energy demands of high-producing beef and dairy cattle. Bacteria are important players in starch digestion in the rumen, and thus play an important role in the hydrolysis and fermentation of cereal grains. However, our understanding of the composition of the rumen starch-hydrolyzing bacteria (SHB) is limited. In this study, BODIPY FL DQ starch staining combined with fluorescence in situ hybridization (FISH) and quantitative FISH were applied to label, identify and quantify SHB possessing active cell-surface-associated (CSA) α-amylase activity in the rumen of heifers fed barley-based diets. When individual cells of SHB with active CSA α-amylase activity were enumerated, they constituted 19-23% of the total bacterial cells attached to particles of four different cultivars of barley grain and corn. Quantitative FISH revealed that up to 70-80% of these SHB were members of Ruminococcaceae in the phylum Firmicutes but were not Streptococcus bovis, Ruminobacter amylophilus, Succinomonas amylolytica, Bifidobacterium spp. or Butyrivibrio fibrisolvens, all of whose amylolytic activities have been demonstrated previously in vitro. The proportion of barley grain in the diet had a large impact on the percentage abundance of total SHB and Ruminococcaceae SHB in these animals.

  19. Effect of forage level and replacing canola meal with dry distillers grains with solubles in precision-fed heifer diets: Digestibility and rumen fermentation.

    Science.gov (United States)

    Suarez-Mena, F X; Lascano, G J; Rico, D E; Heinrichs, A J

    2015-11-01

    Objectives of this study were to determine the effects of feeding differing forage-to-concentrate ratios (F:C) and inclusion rates of corn dry distillers grain with solubles (DDGS) on digestion and rumen fermentation in precision-fed dairy heifer rations. A split-plot design with F:C as whole plot and DDGS inclusion level as sub-plot was administered in a 4-period (19 d) 4 × 4 Latin square. Eight rumen-cannulated Holstein heifers (12.5 ± 0.5 mo of age and 344 ± 15 kg of body weight) housed in individual stalls were allocated to 2 F:C [50:50, low forage, or 75:25 high forage; dry matter (DM) basis] and to a sequence of DDGS inclusion (0, 7, 14, and 21%; DM basis). Forage was a mix of 50% corn silage and 50% grass hay (DM basis). Diets were fed to allow for 800 g/d of body weight gain and fed 1×/d. Rumen contents were sampled at -2, 0, 2, 4, 6, 8, 10, 12, and 20 h after feeding for rumen fermentation measures. Low-forage rations had greater DM and organic matter apparent digestibility. We detected a quadratic effect for DM, organic matter, acid detergent fiber, and neutral detergent fiber apparent digestibility, with the 14% DDGS inclusion level having the highest values. Nitrogen retention decreased with increasing levels of DDGS. Molar proportions of acetate tended to be greater for HF and decreased as DDGS increased; propionate increased as DDGS increased, resulting in the opposite effect on acetate to propionate ratio. Rumen protozoa count decreased as DDGS increased. Moderate levels (14% of DM) of DDGS appear to enhance nutrient utilization and fermentation in precision-fed dairy heifers fed different F:C diets.

  20. An evaluation of the accuracy and precision of methane prediction equations for beef cattle fed high-forage and high-grain diets.

    Science.gov (United States)

    Escobar-Bahamondes, P; Oba, M; Beauchemin, K A

    2017-01-01

    The study determined the performance of equations to predict enteric methane (CH4) from beef cattle fed forage- and grain-based diets. Many equations are available to predict CH4 from beef cattle and the predictions vary substantially among equations. The aims were to (1) construct a database of CH4 emissions for beef cattle from published literature, and (2) identify the most precise and accurate extant CH4 prediction models for beef cattle fed diets varying in forage content. The database was comprised of treatment means of CH4 production from in vivo beef studies published from 2000 to 2015. Criteria to include data in the database were as follows: animal description, intakes, diet composition and CH4 production. In all, 54 published equations that predict CH4 production from diet composition were evaluated. Precision and accuracy of the equations were evaluated using the concordance correlation coefficient (r c ), root mean square prediction error (RMSPE), model efficiency and analysis of errors. Equations were ranked using a combined index of the various statistical assessments based on principal component analysis. The final database contained 53 studies and 207 treatment means that were divided into two data sets: diets containing ⩾400 g/kg dry matter (DM) forage (n=116) and diets containing ⩽200 g/kg DM forage (n=42). Diets containing between ⩽400 and ⩾200 g/kg DM forage were not included in the analysis because of their limited numbers (n=6). Outliers, treatment means where feed was fed restrictively and diets with CH4 mitigation additives were omitted (n=43). Using the high-forage dataset the best-fit equations were the International Panel on Climate Change Tier 2 method, 3 equations for steers that considered gross energy intake (GEI) and body weight and an equation that considered dry matter intake and starch:neutral detergent fiber with r c ranging from 0.60 to 0.73 and RMSPE from 35.6 to 45.9 g/day. For the high-grain diets, the 5 best

  1. 响应面法优化啤酒糟培养基生产蛋白饲料的研究%Optimization of Microbial Media of Brewer Spent Grains of Protein Feed by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    吴明霞; 陈锡雄; 阮少江

    2012-01-01

    To optimize culture medium of the production of protein feed, the effect of the amount of wheat bran, lees and ammonium sulfate weight were studied with Box-Behnken design. The optimum extraction conditions were obtained as follows: dre-wer spent grains 88. 5% , wheat bran 7. 5% , ( NH4 )2 SO4 1%. Under the optimal conditions, the true protein content was 37.64% ,which agreed well with predictive value,indicating the optimized process was feasible.%选取啤酒糟量、麸皮量和硫酸铵量为因子,用响应面法对生产蛋白饲料的培养基进行优化.在单因素试验的基础上,根据Box-Benhnken中心组合方法进行三因素三水平试验.结果表明,最佳工艺条件为啤酒糟88.5%、麸皮7.5%、硫酸铵1%.在此条件下得到的真蛋白含量为37.64%.

  2. SILAGE CANE SUGAR ADDED WITH DRIED BREWER

    Directory of Open Access Journals (Sweden)

    W. J. R. Castro

    2015-02-01

    Full Text Available The objective of this experiment was to evaluate the fermentative parameters and chemical composition of silage cane sugar added with residue dried brewery. The experimental design was completely randomized with four treatments and four replications: 100% cane sugar; 90% of cane sugar + 10% residue dried brewer; 80% of cane sugar + 20% residue dried brewer and 70% cane sugar + 30% dried brewer based on natural matter, composed silages. The sugar cane was chopped in a stationary machine with forage particle size of approximately 2 cm, and homogenized manually with the additives. For storage chopped fresh weight were used in experimental silos capacity of about 4 liters. The results showed that the contents of dry matter and crude protein showed positive linear (P0.05 with mean value of 3.81, while for ether extract and ash results were positive linear (P0.05 for N ammonia presented average value of 4.18. It is concluded that the addition of brewer dehydrated improves the fermentation process of silage cane sugar, in addition to improving their nutritional characteristics.

  3. Effects of balancing crystalline amino acids in diets containing heat-damaged soybean meal or distillers dried grains with solubles fed to weanling pigs.

    Science.gov (United States)

    Almeida, F N; Htoo, J K; Thomson, J; Stein, H H

    2014-10-01

    Two experiments were conducted to investigate if adjustments in diet formulations either based on total analysed amino acids or standardized ileal digestible (SID) amino acids may be used to eliminate negative effects of including heat-damaged soybean meal (SBM) or heat-damaged corn distillers dried grains with solubles (DDGS) in diets fed to weanling pigs. In Experiment 1, four corn-SBM diets were formulated. Diet 1 contained non-autoclaved SBM (315 g/kg), and this diet was formulated on the basis of analysed amino acid concentrations and using SID values from the AminoDat® 4.0 database. Diet 2 was similar to Diet 1 in terms of ingredient composition, except that the non-autoclaved SBM was replaced by autoclaved SBM at 1 : 1 (weight basis). Diet 3 was formulated using autoclaved SBM and amino acid inclusions in the diet were adjusted on the basis of analysed total amino acid concentrations in the autoclaved SBM and published SID values for non-autoclaved SBM (AminoDat® 4.0). Diet 4 also contained autoclaved SBM, but the formulation of this diet was adjusted on the basis of analysed amino acids in the autoclaved SBM and SID values that were adjusted according to the degree of heat damage in this source of SBM. Pigs (160; initial BW: 10.4 kg) were allotted to the four treatments with eight replicate pens per treatment in a randomized complete block design. Diets were fed to pigs for 21 days. The gain to feed ratio (G : F) was greater (PDiet 1 compared with pigs fed the other diets and pigs fed Diet 4 had greater (PDiet 2. In Experiment 2, 144 pigs (initial BW: 9.9 kg) were allotted to four diets with eight replicate pens per diet. The four diets contained corn, SBM (85 g/kg) and DDGS (220 g/kg), and were formulated using the concepts described for Experiment 1, except that heat-damaged DDGS, but not heat-damaged SBM, was used in the diets. Pigs fed Diet 1 had greater (PDiet 2, but no differences were observed for G : F among pigs fed diets containing autoclaved

  4. Long-term reticuloruminal pH dynamics and markers of liver health in early-lactating cows of various parities fed diets differing in grain processing.

    Science.gov (United States)

    Humer, E; Khol-Parisini, A; Gruber, L; Gasteiner, J; Abdel-Raheem, Sh M; Zebeli, Q

    2015-09-01

    The present study aimed to investigate the long-term effect of feeding barley grain steeped in lactic acid (La) with or without thermal treatment on reticuloruminal pH dynamics and metabolic activity of the liver in 12 primiparous and 18 multiparous early-lactating dairy cows. All cows were included on d 21 postpartum and sampled until d 90 postpartum. Cows were fed a diet based on differently processed ground barley grain: untreated grain (control diet, CON), or grain treated with 1% La alone for 24 h before feeding (La), or with an additional oven-heating at 55°C for 12 h (LaH). The reticuloruminal pH and temperature were measured via indwelling sensors that allowed for continuous (every 10min) and long-term measurement from d 21 to 80 postpartum. Blood samples were taken on d 21, 40, and 90 of lactation and analyzed for liver enzymes aspartate aminotransferase (AST), gamma-glutamyltransferase, and glutamate dehydrogenase, as well as bilirubin, bile acids, and serum amyloid A. Dry matter intake was higher in multiparous cows (20.7±0.27 kg/d) compared with primiparous cows (18.2±0.33 kg/d), but was not affected by dietary treatment. Overall, the relatively short duration (51±5min/d) of reticuloruminal pH pH pH pH readings and shorter periods in which the ruminal pH dropped below the threshold of pH 5.8. The reticuloruminal temperature was not affected by dietary treatment, whereas parity affected the time duration of reticuloruminal temperature >39.5°C, being 60±19min/d shorter in primiparous cows. The measured activities of the liver enzymes AST, gamma-glutamyltransferase, and glutamate dehydrogenase, as well as bilirubin, bile acids, and the acute phase protein serum amyloid A, were not affected by grain feeding. Additionally, only one small effect of parity on investigated serum variables was noticed, showing slightly but significantly higher values of AST in multiparous (80.5±1.4 U/L) compared with primiparous cows (76.0±1.7 U/L). In conclusion, our

  5. Live production and carcass characteristics of broilers fed a blend of poultry fat and corn oil derived from distillers dried grains with solubles.

    Science.gov (United States)

    Kim, E J; Purswell, J L; Davis, J D; Loar, R E; Karges, K

    2013-10-01

    Corn distillers dried grains with solubles (DDGS) are now being further processed to remove corn oil, which may be used as a dietary energy source for poultry. The objectives of this study were to compare the effects of a poultry fat (PF) and a new DDGS-derived corn oil blend (CO) on live performance and carcass characteristics of 49-d-old broilers. Four corn-soybean meal based diets were formulated with differing blends of PF and CO. All diets contained the same percentage of total fat, but differed in the fat source. One diet had the sole source of fat as PF (100:0% PF:CO) and was then replaced with 25% CO, 75% CO, and a 100% replacement of CO. Each of the diets was fed in a 3-phase feeding program to 6 replicate pens. At day of hatch, Ross × Ross 708 broilers were randomly allocated to 24 pens composed of 42 birds of equal sex. On d 49, 10 birds from each pen were processed, and carcass, abdominal fat pad, and breast muscle components were determined. There were no significant differences in live performance for the starter phase (0-18 d). For the grower phase (19-35 d), birds fed 75:25% PF:CO significantly (P ≤ 0.05) increased BW, BW gain, and decreased feed conversion compared with the control (100:0% PF:CO). Birds fed 0:100% PF:CO also observed similar improvements in BW, BW gain, and feed conversion during the grower phase. There were no significant differences for the finisher phase (36-48 d). On d 49, live weights for birds fed the 0:100% PF:CO diets were significantly lower compared with other treatments. A trend for lower carcass and breast weights and increased abdominal fat was also observed for birds fed the 0:100% PF:CO. The addition of CO led to significant improvements in pellet durability for grower and finisher pellets. The results of this study indicate that DDGS-derived CO can be used to partially replace PF in broiler diets without any detrimental effects.

  6. Supplementation of corn dried distillers grains plus solubles to gestating beef cows fed low-quality forage: I. Altered intake behavior, body condition, and reproduction.

    Science.gov (United States)

    Kennedy, V C; Bauer, M L; Swanson, K C; Vonnahme, K A

    2016-01-01

    To investigate the effects of corn dried distillers grains plus solubles (DDGS) supplementation to cows fed corn stover and silage during late gestation, 27 multiparous beef cows (674 ± 17 kg; BCS, 5.6 ± 0.1) were divided randomly into 2 pens equipped with electronic feeders. For 10 wk, both groups were fed the basal diet for ad libitum intake while 1 group was supplemented (SUP; = 12) with DDGS at 0.3% of BW (DM basis). Following parturition, all cows received the same diet for an additional 8 wk. During gestation, SUP cows gained BW ( time spent consuming forage ( meals than SUP cows ( = 0.06) from d 201 to 218 of gestation. Supplemented cows tended ( = 0.09) to consume larger meals than CON cows and spent more ( time eating than CON cows around d 240 of gestation. Calves born to SUP cows tended ( = 0.06) to be heavier than calves born to CON cows. During lactation, both groups gained ( time but was not influenced ( = 0.44) by treatment. Supplemented cows spent more time ( meals increased with advancing lactation ( meals daily than SUP cows ( = 0.01). Conversely, meal size decreased as lactation advanced ( meals than CON cows ( = 0.05). Supplementation with DDGS during gestation influenced intake behavior during gestation and lactation as well as the maintenance of maternal BW and BCS and calf birth BW.

  7. Use of dicarboxylic acids and polyphenols to attenuate reticular pH drop and acute phase response in dairy heifers fed a high grain diet.

    Science.gov (United States)

    De Nardi, Roberta; Marchesini, Giorgio; Plaizier, Jan C; Li, Shucong; Khafipour, Ehsan; Ricci, Rebecca; Andrighetto, Igino; Segato, Severino

    2014-11-26

    The aim of this study was to determine the ability of two feed additives, a fumarate-malate (FM) and a polyphenol-essential oil mixture (PM), in attenuating the drop of ruminal pH and the metabolic and immune response resulting from an excessively high grain diet. Six heifers were used in a 3 × 3 Latin square experiment and fed a low starch (LS) diet for 14 d, followed by a high starch (HS) diet for 8 d (NDF 33.6%, starch 30.0% DM). In the last 5 days of each period, barley meal was added to decrease rumen pH. During HS feeding all animals were randomly assigned to one of the following three dietary treatments: no supplement/control (CT), a daily dose of 60 g/d of FM, or 100 g/d of PM. Reticular pH was continuously recorded using wireless boluses. On d 21 of each period, rumen fluid was collected by rumenocentesis (1400 h), together with blood (0800 h) and fecal samples (0800, 1400, and 2100 h). The correlation coefficient of pH values obtained using the boluses and rumenocentesis was 0.83. Compared with CT and PM, the FM treatment led to a lower DMI. Nadir pH was lowest during CT (5.40, 5.69, and 5.62 for CT, FM and PM, respectively), confirming the effectiveness of both supplements in reducing the pH drop caused by high grain feeding. This result was confirmed by the highest average time spent daily below 5.6 pH (199, 16 and 18 min/d) and by the highest acetate to propionate ratio of the CT fed heifers. The PM decreased the concentrations of neutrophils (2.9, 3.2, and 2.8 10(9)/L) and acute phase proteins: SAA (37.1, 28.6 and 20.1 μg/mL), LBP (4.1, 3.8, and 2.9 μg/mL), and Hp (675, 695 and 601 μg/mL). Free lipopolysaccharides (LPS) were detected in blood and feces, but their concentrations were not affected by treatments, as the remaining blood variables. Data suggest that both additives could be useful in attenuating the effects of excessive grain feeding on rumen pH, but the PM supplement was more effective than FM in reducing the inflammatory response

  8. Pork fat quality of pigs fed distillers dried grains with solubles with variable oil content and evaluation of iodine value prediction equations.

    Science.gov (United States)

    Wu, F; Johnston, L J; Urriola, P E; Shurson, G C

    2016-03-01

    Back, belly, and jowl fat samples of pigs fed control corn-soybean meal-based diets and diets containing 4 sources of distillers dried grains with solubles (DDGS) were used to determine the impact of feeding DDGS with variable oil content on pork fat quality and to evaluate the precision and accuracy of published iodine value (IV) prediction equations. Dietary treatments consisted of 4 corn-soybean meal diets containing 40% DDGS from different sources with 10.7, 5.6, 14.2, or 16.0% ether extract (EE; as-fed) content. Diets did not contain any other supplemental lipid sources. Regardless of fat depot, SFA content (g/100 g fat) of pigs fed 5.6% EE DDGS (35.4) was greater ( fat depot, MUFA content (g/100 g fat) of pigs fed 10.7, 5.6, and 14.2% DDGS sources were similar (43.7, 43.1, and 43.0, respectively) but were greater ( fat depot interaction was observed for PUFA ( oil content of DDGS was greater in backfat than in belly and jowl fat. Carcass fat IV data were used to evaluate prediction error (PE) and bias of published carcass fat IV prediction equations. Equations using dietary C18:2 content or IV product as a single predictor resulted in highly variable PE (g/100 g) ranging from 3.43 to 8.36 and bias (g/100 g) ranging from -5.05 to 5.66. Using equations that included additional diet composition information and pig growth performance factors decreased PE (3.27 to 4.73) and bias (-3.37 to 1.73) of prediction for backfat compared with equations only based on the characteristics of dietary lipid, but this improvement was limited in the prediction for belly and jowl fat. Predictions based on percentage of DDGS in diets had the greatest PE (6.66 to 9.19) and bias (5.53 to 8.00).

  9. Consumo e digestibilidade em ovinos alimentados com grãos e subprodutos da canola Intake and digestibility of sheep fed grains and by-products of canola

    Directory of Open Access Journals (Sweden)

    Priscila Silva Neubern de Oliveira

    2009-03-01

    Full Text Available Objetivou-se avaliar a inclusão de 8% de grãos e subprodutos da canola (farelo ou torta nas dietas sobre o consumo e a digestibilidade. Seis ovinos machos não-castrados da raça Santa Inês (210 e 240 dias de idade e peso corporal de 44,8 + 4,2kg receberam dietas contendo 40% de feno de capim Tifton e 60% de concentrado composto por milho em grão, farelo de soja, mistura mineral, além de canola em grão integral, farelo de canola e torta de canola, que constituíram os três tratamentos. Não houve diferença (P>0,05 para o consumo de matéria seca (MS, matéria orgânica (MO, extrato etéreo (EE, energia bruta (EB, fibra em detergente neutro (FDN, carboidratos totais (CT e carboidratos não-fibrosos (CNF entre as dietas experimentais, no ensaio de digestibilidade. Não houve efeito (P>0,05 de tratamento para a digestibilidade de MS, MO, EE, PB, EB, FDN, CT e CNF. Recomenda-se incluir até 8% de grãos e subprodutos da canola (farelo ou torta na dieta de ovinos.The effect of feeding 8% of grains and byproducts (meal or cake of canola on intake and digestibility was evaluated. Six non castrated Santa Ines sheep (from 210 to 240 days old and body weight of 44.8 + 4.2kg were fed diets composed by 40% of Tifton hay and 60% of concentrate based on corn grain, soybean meal, whole grain canola, canola meal, canola cake and mineral mixture. No differences on the intakes of dry matter (DM, organic matter (OM, ether extract (EE, gross energy (GE, neutral detergent fiber (NDF, total carbohydrates (TC and non fiber carbohydrate (NFC were observed among treatments, in the digestibility trial. No treatment effect on the digestibilities of DM, OM, EE, CP, GE, NDF, TC and NFC was observed. It is recommended to include up to 8% of grains and byproducts (meal or cake of canola in the sheep diet.

  10. Feeding high-moisture corn grain silage to broilers fed alternative diets and maintained at different environmental temperatures

    Directory of Open Access Journals (Sweden)

    VC Cruz-Polycarpo

    2014-12-01

    Full Text Available The effects of the dietary substitution of dry corn by high-moisture corn grain silage (HMCGS were evaluated on the performance, nutrient digestibility and serum biochemical parameters of broilers reared in an alternative production system and submitted to different environmental temperatures. A total of 288 one-day-old male Cobb chicks were distributed according to a randomized block design in a 3x4 factorial arrangement: three environmental temperatures (hot, thermoneutral or cold and four levels of HMCGS in substitution of dry corn (0%, 20%, 40% or 60%. The acid analysis showed that the evaluated HMCGS contained average percentage values of ethanol, lactic acid, and acetic acid (expressed in 100% of dry matter of 0.7690, 2.7320 and 0.0249%, respectively. Propionic and butyric acids were not detected. Dry corn and HMCGS presented pH values of 5.8 and 3.3, respectively. The inclusion of HMCGS reduced dietary pH, as shown by the values of 5.7, 5.4, 5.1 and 4.8 recorded for the diets containing 0%, 20%, 40% and 60% of HMCGS, respectively. There was no significant interaction between diets and environmental temperature. HMCGS may replace up to 40% dry corn in broiler diets when performance, triglyceride levels, and HDL-cholesterol ratio is considered, and up to 60% when nutrient digestibility is evaluated. High environmental temperature impairs broiler performance, nutrient digestibility, and serum biochemistry, demonstrating the influence of environmental temperature on broiler metabolism and performance.

  11. Comparison of values for standardized total tract digestibility and relative bioavailability of phosphorus in dicalcium phosphate and distillers dried grains with solubles fed to growing pigs.

    Science.gov (United States)

    Baker, S R; Kim, B G; Stein, H H

    2013-01-01

    Two experiments were conducted to compare values for the standardized total tract digestibility (STTD) and the relative bioavailability of P in dicalcium phosphate (DCP) and distillers dried grains with solubles (DDGS) when fed to growing pigs. In Exp. 1, the apparent total tract digestibility (ATTD), the basal endogenous P loss (EPL), and the STTD of P in DCP and DDGS were determined. Eighteen pigs (initial BW: 34.93±1.04 kg) were allotted to 3 cornstarch-based diets in a randomized complete block design and housed individually in metabolism cages. Two diets contained DCP and DDGS, respectively, as the sole source of P and the last diet was a P-free diet that was used to measure EPL from the pigs. Results indicated that the ATTD of P in DCP and DDGS were 86.1 and 58.8%, respectively, and the STTD of P in DCP and DDGS were 93.1 and 63.1%, respectively. The EPL was determined at 174 mg/kg DMI. In Exp. 2, 42 pigs (initial BW: 29.02±2.03 kg) were allotted to 7 dietary treatments in a randomized complete block design. Pigs were housed individually and allowed ad libitum access to feed and water. A basal diet (0.22% P) based on corn, casein, cornstarch, and potato protein concentrate was formulated. Three additional diets were formulated by adding 0.04, 0.08, or 0.12% P from DCP to the basal diet to create diets containing 0.26, 0.30, or 0.34% P. The last 3 diets were formulated by adding 0.04, 0.08, or 0.12% P from DDGS to the basal diet at the expense of cornstarch. Pigs were fed experimental diets for 28 d. They were then euthanized and the third and fourth metacarpals from the right front foot were collected. Metacarpal bone ash and bone P were regressed against P intake for each ingredient and via slope ratio methodology, it was determined that the bioavailability of P in DDGS was 87% relative to that in DCP. It was concluded from this work that the value for relative bioavailability of P in DDGS overestimates the digestibility of P in DDGS and values for the

  12. 27 CFR 28.227 - Removals of beer by persons other than the brewer or agent of the brewer.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Removals of beer by... ALCOHOL Exportation of Beer With Benefit of Drawback Execution of Claims § 28.227 Removals of beer by persons other than the brewer or agent of the brewer. Where there is a removal of taxpaid beer by a...

  13. Study on production of protein feedstuff from apple pomace and brewer's grains by cooperation of different microbial strains%多菌种协同发酵啤酒糟渣和苹果渣生产蛋白饲料的研究

    Institute of Scientific and Technical Information of China (English)

    王晓力; 王帆; 孙尚琛; 王永刚; 李想; 王春梅; 朱新强; 孙启忠

    2016-01-01

    Optimization the solid-state fermentation process was studied by single factor experiment and orthogonal experiment using apple pomace and brewer's grains as fermentation substrate, and Trichodermareesei, Aspergillus niger and Candida utilis as for fermentation strains. As well as, evalua⁃tion of the fermented feedstuff quality and exploration the role of microorganisms in solid-state fermen⁃tation using scanning electron microscopy were investigated. The results showed that the proportion of fermentation substrate and three microorganisms were 1��1 and 1��1��1 respectively, inoculum amount was 8%,the water content, fermentation temperature and time was 60%,35 ℃ and 48 h. Un⁃der optimized conditions,the protein content was increased 34.52%, while the acidic and neutral cellu⁃lose content were decreased 22.93% and 9.08% respectively. The feedstuff with a fantastic quality and no agglomerate was accompanied by mild acid and apple aroma. The degradability of crude protein, acidic and neutral cellulose in feedstuff using the nylon-bag method in the sheep rumen were 79.78%,51.96% and 39.88% respectively that 72 h after. Scanning electron microscopy results indicated that the microbes in the fermentation process destroyed the surface structure of feed⁃stuff , amorphous area of cellulose molecules dis⁃persed, and a large number of yeast cells distrib⁃uted in the surface of the feedstuff to absorb nu⁃ trients growing to feedstuff with high protein content.%以苹果渣和啤酒糟为发酵基质,利用里氏木霉、黑曲霉、产朊假丝酵母为发酵菌种,采用单因素3验和正交试验对固态发酵工艺进行了优化,并对发酵饲料品质进行了评定,利用扫描电子显微镜技术探究了微生物在固态发酵中的作用。结果表明,最佳的基质配比为1��1,3种微生物配比为1��1��1,接种量为8%,含水量、发酵温度和时间分别为60%,35℃和48 h,在此条件下,

  14. Pseudoporphyria associated with consumption of brewers' yeast.

    Science.gov (United States)

    Lim, C K; Rideout, J M; Peters, T J

    1984-06-01

    A case of pseudoporphyria associated with excessive consumption of brewers ' yeast was studied. Detailed analysis of the yeast tablets by high performance liquid chromatography showed the presence of dicarboxylic deuteroporphyrin , mesoporphyrin, and protoporphyrin; coproporphyrin I and III isomers; and uroporphyrin I and III isomers. The faecal porphyrin concentration of the patient taking yeast tablets was significantly increased, resembling the excretion pattern in variegate porphyria. Any patient showing an unusual porphyrin excretion pattern on high performance liquid chromatography should be investigated for a possible dietary cause.

  15. Capacity of Fusarium species isolated from brewer's barley to synthesise zearalenone

    Directory of Open Access Journals (Sweden)

    Kocić-Tanackov Sunčica D.

    2005-01-01

    Full Text Available Fungi of the genus Fusarium, known as toxigenic species, are very of- ten parasites and contaminants of brewer's barley. In this paper, the composition of the genus Fusarium species in brewer's barley samples and their potential in the zearalenone synthesis were investigated. The tests were done on different brewer's barley varieties, crop 2003, samples (SSK1, SSK2, SSK3 SSK4, SSK5, SSK6, SSK7, SSK8, SSK9, SSK10 and SSK12 from Kragujevac locality. The isolation and identification of the Fusarium species were done according to the methods described by N e l s o n et al. (1983. The identified Fusarium species (6 were tested for their capacity to synthesise zearalenone. The isolates were cultivated on sterilised barley grains at the temperature of 25°C for 14 days, and then the zearalenone concentration was determined by the fluorometric method on the fluorometer "VI- CAM" series 4. The following seven Fusarium species were isolated from barley samples: F. acuminatum, F. avenaceum, F. culmorum, F. equiseti, F. poae, F. sporotrichioides and F. tricinctum. F. poae was the most distributed species (10.26%. The zearalenone concentration within the range of 12.0 to 430.0 g kg-1 was determined in cultures of barley grain inoculated with F. avenacuem (SSK6 and SSK12, F. culmorum (SSK8, F. tricinctum (SSK1, F. sporotrichioides (SSK7 and SSK12 and F. poae (SSK5, SSK9 and SSK10. Isolates of F. equiseti (SSK2 and F. poae (SSK6 did not express capacity to synthesise this toxic metabolite.

  16. Charles L. Brewer Award for Distinguished Teaching of Psychology

    Science.gov (United States)

    American Psychologist, 2007

    2007-01-01

    This article announces the 2007 recipient of the Charles L. Brewer Award for Distinguished Teaching of Psychology: Baron Perlman. A brief biography, highlighting areas of special focus in Perlman's work, is provided.

  17. EFFECTS OF MILLET MALT WORT ON BREWER'S YEAST

    African Journals Online (AJOL)

    BSN

    Barely malt has been the brewers choice for beer fermentation. (1'. ... Peptone yeast extract glucose broth prepared according to the methods of .... satisfactory as the crop of yeast recovered was high and responded well in fermentation to the ...

  18. Persistence of Escherichia coli O157:H7 and Total Escherichia coli in Feces and Feedlot Surface Manure from Cattle Fed Diets with and without Corn or Sorghum Wet Distillers Grains with Solubles.

    Science.gov (United States)

    Berry, Elaine D; Wells, James E; Varel, Vincent H; Hales, Kristin E; Kalchayanand, Norasak

    2017-08-01

    Feeding corn wet distillers grains with solubles (WDGS) to cattle can increase the load of Escherichia coli O157:H7 in feces and on hides, but the mechanisms are not fully understood. The objective of these experiments was to examine a role for the persistence of E. coli O157:H7 in the feces and feedlot pen surfaces of cattle fed WDGS. In the first study, feces from steers fed 0, 20, 40, or 60% corn WDGS were inoculated with E. coli O157:H7. The E. coli O157:H7 numbers in feces from cattle fed 0% corn WDGS rapidly decreased (P < 0.05), from 6.28 to 2.48 log CFU/g of feces by day 14. In contrast, the E. coli O157:H7 numbers in feces from cattle fed 20, 40, and 60% corn WDGS were 4.21, 5.59, and 6.13 log CFU/g of feces, respectively, on day 14. A second study evaluated the survival of E. coli O157:H7 in feces from cattle fed 0 and 40% corn WDGS. Feces were collected before and 28 days after the dietary corn was switched from high-moisture corn to dry-rolled corn. Within dietary corn source, the pathogen persisted at higher concentrations (P < 0.05) in 40% corn WDGS feces at day 7 than in 0% WDGS. For 40% corn WDGS feces, E. coli O157:H7 persisted at higher concentrations (P < 0.05) at day 7 in feces from cattle fed high-moisture corn (5.36 log CFU/g) than from those fed dry-rolled corn (4.27 log CFU/g). The percentage of WDGS had no effect on the E. coli O157:H7 counts in feces from cattle fed steam-flaked corn-based diets containing 0, 15, and 30% sorghum WDGS. Greater persistence of E. coli O157:H7 on the pen surfaces of animals fed corn WDGS was not demonstrated, although these pens had a higher prevalence of the pathogen in the feedlot surface manure after the cattle were removed. Both or either the greater persistence and higher numbers of E. coli O157:H7 in the environment of cattle fed WDGS may play a part in the increased prevalence of E. coli O157:H7 in cattle by increasing the transmission risk.

  19. Genetic improvement of brewer's yeast: current state, perspectives and limits.

    Science.gov (United States)

    Saerens, Sofie M G; Duong, C Thuy; Nevoigt, Elke

    2010-05-01

    Brewer's yeast strain optimisation may lead to a more efficient beer production process, better final quality or healthier beer. However, brewer's yeast genetic improvement is very challenging, especially true when it comes to lager brewer's yeast (Saccharomyces pastorianus) which contributes to 90% of the total beer market. This yeast is a genetic hybrid and allopolyploid. While early studies applying traditional genetic approaches encountered many problems, the development of rational metabolic engineering strategies successfully introduced many desired properties into brewer's yeast. Recently, the first genome sequence of a lager brewer's strain became available. This has opened the door for applying advanced omics technologies and facilitating inverse metabolic engineering strategies. The latter approach takes advantage of natural diversity and aims at identifying and transferring the crucial genetic information for an interesting phenotype. In this way, strains can be optimised by introducing "natural" mutations. However, even when it comes to self-cloned strains, severe concerns about genetically modified organisms used in the food and beverage industry are still a major hurdle for any commercialisation. Therefore, research efforts will aim at developing new sophisticated screening methods for the isolation of natural mutants with the desired properties which are based on the knowledge of genotype-phenotype linkage.

  20. EFFECT OF REPLACING CORN AND SOYBEAN MEAL WITH BREWERS RICE AND DRIED DISTILLERS BREWERS YEAST ON PERFORMANCE OF GROWING-FINISHING PIGS

    Directory of Open Access Journals (Sweden)

    Ondieki Gekara

    2014-01-01

    Full Text Available The objective of this study was to determine the effect of replacing corn and Soybean Meal (SBM with Brewers Rice (BR and Dried Distillers Brewers Yeast (DDBY, respectively, on ADG, G:F, Apparent Total Tract Digestibility (ATTD, fecal DM output and fecal loss of N and P of growing-finishing pigs. Sixty four Yorkshire x Duroc x Hampshire crosses (BW = 73±5.7 kg were randomly assigned to corn/SBM (CSM, BR/SBM (RSM, corn/DDBY (CBY or BR/DDBY (RBY diets. Compared with pigs finished on corn based diets, pigs fed BR based diets gained faster (0.868 vs. 0.730 kg.pig-1; p<0.01 and had better gain to feed ratio (0.30 vs. 0.25; p<0.01. Pigs finished on RBY diet had the least fecal DM output (0.245, 0.352, 0.575, 0.639 kg.pig-1; p<0.001 and greatest ATTD (91.5, 87.8, 80.0, 77.9%; p<0.001 followed by RSM, CBY and CSM pigs, respectively. Pigs finished on RBY diet lost the least (p<0.001 amount of N (0.010, 0.013, 0.019, 0.021 kg.pig-1 and P (0.010, 0.014, 0.016, 0.019 kg.pig-1 in the feces followed by pigs finished on RSM, CBY and CSM, respectively. In conclusion, BR and DDBY can replace all corn and SBM in swine diets with no negative effects on performance of growing-finishing pigs.

  1. Effects of distillers dried grains with solubles on amino acid, energy, and fiber digestibility and on hindgut fermentation of dietary fiber in a corn-soybean meal diet fed to growing pigs.

    Science.gov (United States)

    Urriola, P E; Stein, H H

    2010-04-01

    The objective of this experiment was to measure the effect of distillers dried grains with solubles (DDGS) on the digestibility of AA, energy, and fiber, on the fermentation of fiber, and on the first appearance of digesta at the end of the ileum, in the cecum, and in the feces of growing pigs fed a corn-soybean meal-based diet. Sixteen pigs (initial BW = 38.0 +/- 1.6 kg) were prepared with a T-cannula in the distal ileum and a T-cannula in the cecum and allotted to 2 treatments. In period 1, all pigs were fed a corn-soybean meal diet. In periods 2, 3, and 4, pigs were fed the control diet or a diet containing corn, soybean meal, and 30% DDGS. First appearance of digesta at the end of the ileum, in the cecum, and over the entire intestinal tract was measured at the end of period 4. The apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of nutrients were measured, and the concentration of VFA was analyzed in ileal, cecal, and fecal samples. The AID of Lys (74.1%) in the DDGS diet was less (P dietary fiber (TDF) were not different between the 2 diets. The ATTD of GE (81.0%), NDF (57.2%), TDF (55.5%), and DM (81.7%) were less (P pigs fed the 2 diets. The pH of ileal and cecal digesta from pigs fed the DDGS diet (6.3 and 5.5) was greater (P pigs fed the control diet (5.8 and 5.3). The ATTD of DM, GE, ADF, NDF, and TDF did not change with collection period, but the AID of ADF, NDF, and TDF increased (P pigs fed the diet containing DDGS had less digestibility of Lys, GE, ADF, NDF, and TDF than pigs fed the control diet. The digestibility of DM and GE was not influenced by collection period, but the concentration of VFA in cecal digesta and feces increased with the length of time pigs received the diets.

  2. Activation of waste brewer's yeast Saccharomyces cerevisiae for bread production

    Directory of Open Access Journals (Sweden)

    Popov Stevan D.

    2005-01-01

    Full Text Available The waste brewer's yeast S. cerevisiae (activated and non-activated was compared with the commercial baker's yeast regarding the volume of developed gas in dough, volume and freshness stability of produced bread. The activation of waste brewer's yeast resulted in the increased volume of developed gas in dough by 100% compared to non-activated brewer's yeast, and the obtained bread is of more stable freshness compared to bread produced with baker's yeast. The activation of BY affects positively the quality of produced bread regarding bread volume. The volume of developed gas in dough prepared with the use of non-activated BY was not sufficient, therefore, it should not be used as fermentation agent, but only as an additive in bread production process for bread freshness preservation. Intense mixing of dough results in more compressible crumb 48 hrs after baking compared to high-speed mixing.

  3. Ochratoxin A in brewer's yeast used as food supplement.

    Science.gov (United States)

    Gottschalk, Christoph; Biermaier, Barbara; Gross, Madeleine; Schwaiger, Karin; Gareis, Manfred

    2016-02-01

    Brewer's yeasts are rich in vitamins of the B-group and contain other nutritive factors; therefore, they are recommended as valuable food supplements for people with special dietary requirements like pregnant women, children, and adolescents, or for people with high physical activity. Additionally, certain strains of brewer's yeast are known to be capable of adsorbing xenobiotics such as mycotoxins. Because of that, these yeasts are regarded as having positive effects in food, beverage, and feed technology. Their potential to bind mycotoxins such as ochratoxin A (OTA), however, can subsequently lead to a contamination of such brewer's yeasts used as food supplements. In the present study, we analyzed 46 samples of brewer's yeasts for the occurrence of OTA by HPLC with fluorescence detector (HPLC-FLD) and for confirmatory measurements by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Nearly 90% of the samples were contaminated with OTA, the levels ranging from the limit of detection (LOD, 0.01 μg/kg) to 4.2 μg/kg. The mean and median levels of contamination were 0.49 and 0.27 μg/kg, respectively. Based on these results, the additional weekly OTA exposure by regularly consuming such supplements was assessed. Depending on different subpopulations (adults, children) and levels of contamination used for calculation, the additional OTA intake via brewer's yeast products ranged from 9.3% (mean case) to 114% (worst case) of the published mean weekly OTA intake in Germany (adults 279.3 ng, children 195.3 ng). At present, maximum levels for OTA in nutritional supplements like brewer's yeast do not exist. Based on our results, however, it is recommended that producers of these dietary supplements should include mycotoxin analyses in ongoing and future self-monitoring programs and in product quality checks.

  4. Effects of twenty percent alkaline-treated corn stover without or with yucca extract on performance and nutrient mass balance of finishing steers fed modified distillers grains-based diets.

    Science.gov (United States)

    Johnson, J M; Shreck, A L; Nuttelman, B L; Burken, D B; Erickson, G E; Rincker, M J; Cecava, M J; Klopfenstein, T J

    2015-06-01

    Two experiments were conducted with 192 steers each (during the winter [November to May] or summer [June to October]) to evaluate 3 diets with or without Yucca schidigera extract in a 3 × 2 factorial on steer growth performance and N mass balance. One factor was diet (DM basis): 1) 5% untreated corn stover, 51% corn, and 40% modified distillers grains plus solubles (MDGS; CON); 2) 20% calcium oxide-treated corn stover (CaO added at 5% of stover DM), 40% MDGS, and 36% corn (TRT); or 3) 20% untreated corn stover, 40% MDGS, and 36% corn (NONTRT). The other factor was dietary extract at 0 (NOYE) or 1.0 g/d per steer (YE). No interaction between diet and YE was detected (P > 0.51) for growth performance and carcass traits in winter and only for DMI in summer. Final BW, ADG, DMI, or G:F were not different (P ≥ 0.28) between cattle fed CON and TRT, whereas cattle fed NONTRT had lesser ADG, HCW, and G:F compared to CON and TRT in the winter experiment. During the summer, final BW and ADG tended to be greater (P ≥ 0.07) for CON compared to TRT. Cattle fed TRT had reduced (P 0.18) on amount (kg/steer) or percentage of N volatized in the winter or summer. All diets had similar amounts (P > 0.13) of DM and OM removed from the pen surface in both summer and winter. Feeding CaO-treated corn stover as a partial grain replacement had no impact on performance in winter but decreased G:F in summer. Although high-fiber diets increased the amount of OM on pen surfaces, they did not impact N volatilized. Feeding a Y. schidigera extract did not affect N balance or manure characteristics.

  5. 27 CFR 25.173 - Brewer in default.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Brewer in default. 25.173 Section 25.173 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... consumption or sale or taken from the brewery for consumption or sale until the tax has been prepaid...

  6. Charles L. Brewer award for distinguished teaching of psychology.

    Science.gov (United States)

    2010-01-01

    The American Psychological Foundation (APF) Charles L. Brewer Award for Distinguished Teaching of Psychology recognizes an outstanding career contribution to the teaching of psychology. The 2010 recipient of the Distinguished Teaching Award is Bernard C. Beins. This article recognizes his life achievement in the area of psychology.

  7. Differences in Circulating microRNAs between Grazing and Grain-Fed Wagyu Cattle Are Associated with Altered Expression of Intramuscular microRNA, the Potential Target PTEN, and Lipogenic Genes.

    Science.gov (United States)

    Muroya, Susumu; Shibata, Masahiro; Hayashi, Masayuki; Oe, Mika; Ojima, Koichi

    2016-01-01

    We aimed to understand the roles of miRNAs in the muscle tissue maturation and those of circulating microRNAs (c-miRNAs) in beef production of Japanese Black (JB) cattle (Wagyu), a breed with genetically background of superior intermuscular fat depot, by comparing different feeding conditions (indoor grain-feeding vs. grazing on pasture). The cattle at 18 months old were assigned to pasture feeding or conventional indoor grain feeding conditions for 5 months. Microarray analysis of c-miRNAs from the plasma extracellular vesicles led to the detection of a total of 202 bovine miRNAs in the plasma, including 15 miRNAs that differed between the feeding conditions. Validation of the microarray results by qPCR showed that the circulating miR-10b level in the grazing cattle was upregulated compared to that of the grain-fed cattle. In contrast, the levels of miR-17-5p, miR-19b, miR-29b, miR-30b-5p, miR-98, miR-142-5p, miR-301a, miR-374b, miR-425-5p, and miR-652 were lower in the grazing cattle than in the grain-fed cattle. Bioinformatic analysis indicated that the predicted target genes of those c-miRNAs were enriched in gene ontology terms associated with blood vessel morphogenesis, plasma membrane, focal adhesion, endocytosis, collagen, ECM-receptor interaction, and phosphorylation. In the grazing cattle, the elevation of miR-10b expression in the plasma was coincident with its elevation in the longissimus lumborum (LL) muscle. Expression of bovine-specific miR-2478, the most plasma-enriched miRNA, tended to be also upregulated in the muscle but not in the plasma. Furthermore, grazing caused the downregulated mRNA expression of predicted miR-10b and/or miR-2478 target genes, such as DNAJB2, PTEN, and SCD1. Thus, the feeding system used for JB cattle affected the c-miRNAs that could be indicators of grain feeding. Among these, miR-10b expression was especially associated with feeding-induced changes and with the expression of the potential target genes responsible for

  8. Emissions of greenhouse gases, ammonia, and hydrogen sulfide from pigs fed standard diets and diets supplemented with dried distillers grains with solubles

    Science.gov (United States)

    Swine growers are increasingly supplementing animal diets with dried distillers grains soluble (DDGS) to offset cost of a typical corn-soybean meal diet. An experiment was conducted to investigate the effects of DDGS diets on both on manure composition and emissions of greenhouse gases (GHG), ammoni...

  9. Phosphorus utilization and characterization of ileal digesta and excreta from broiler chickens fed diets varying in cereal grain, phosphorus level, and phytase addition

    Science.gov (United States)

    Both endogenous and exogenous phytase present in poultry feeds can alter phytate utilization as well as the solubility of phosphorus (P) excreted, which can be a concern from an environmental perspective. To address the effects of endogenous phytase present in cereal grains and exogenous phytase ad...

  10. Products deriving from microbial fermentation are linked to insulinaemic response in pigs fed breads prepared from whole-wheat grain and wheat and rye ingredients

    DEFF Research Database (Denmark)

    Theil, Peter Kappel; Jørgensen, Henry Johs. Høgh; Serena, Anja;

    2011-01-01

    The effects of wheat and rye breads made from whole-wheat grain (WWG), wheat aleurone flour (WAF) or rye aleurone flour (RAF) on net portal absorption of carbohydrate-derived nutrients (glucose, SCFA and lactate) and apparent insulin secretion were studied in a model experiment with catheterised...

  11. Supplementation of organic and inorganic selenium to diets using grains grown in various regions of the United States with differing natural Se concentrations and fed to grower-finisher swine.

    Science.gov (United States)

    Mahan, D C; Azain, M; Crenshaw, T D; Cromwell, G L; Dove, C R; Kim, S W; Lindemann, M D; Miller, P S; Pettigrew, J E; Stein, H H; van Heugten, E

    2014-11-01

    Grains grown in various regions of the United States vary in their innate or natural Se contents. A regional study evaluated the effects of adding inorganic Se (sodium selenite) or organic Se (Se yeast) to diets with differing innate Se contents. A 2 × 2 + 1 factorial experiment evaluating 2 Se sources (organic or inorganic) at 2 Se levels (0.15 or 0.30 mg/kg) in 18 total replicates (n = 360 total pigs). A basal diet was fed without supplemental Se and served as the negative (basal) control. The study was conducted as a randomized complete block design in 9 states (Georgia, Illinois, Kentucky, Nebraska, North Carolina, Ohio, South Dakota, Texas, and Wisconsin) with each station conducting 2 replicates. Pigs were fed from 25 to approximately 115 kg BW. Similar dietary formulations were used at each station, incorporating a common source of trace mineral and Se premixes. Three pigs per treatment in 16 replicates (n = 240) were bled at 55, 85, and 115 kg BW and serum Se and glutathione peroxidase (GSH-Px) activities were determined. Three pigs (n = 260) from each treatment pen were killed at 115 kg BW and issues (liver, loin, and hair) were analyzed for Se. The corn Se content from the various states ranged from 0.026 to 0.283 mg Se/kg while the soybean meal Se content ranged from 0.086 to 0.798 mg Se/kg. Tissue and serum Se concentrations were greater (P supplemental organic Se was fed, whereas serum GSH-Px was greater (P dietary Se level increased within each state. There was a source × level interaction (P dietary Se level indicating that those states having greater grain natural Se contents also had greater tissue Se concentrations. These results indicate that a large difference in corn and soybean meal Se concentrations exists between states, that the addition of organic or inorganic Se to these grains increased tissue and serum Se in each state, and that organic Se was incorporated at greater concentrations in the loin, liver, and hair tissues of grower

  12. Illumina Sequencing Approach to Characterize Thiamine Metabolism Related Bacteria and the Impacts of Thiamine Supplementation on Ruminal Microbiota in Dairy Cows Fed High-Grain Diets

    Directory of Open Access Journals (Sweden)

    Xiaohua Pan

    2017-09-01

    Full Text Available The requirements of thiamine in adult ruminants are mainly met by ruminal bacterial synthesis, and thiamine deficiencies will occur when dairy cows overfed with high grain diet. However, there is limited knowledge with regard to the ruminal thiamine synthesis bacteria, and whether thiamine deficiency is related to the altered bacterial community by high grain diet is still unclear. To explore thiamine synthesis bacteria and the response of ruminal microbiota to high grain feeding and thiamine supplementation, six rumen-cannulated Holstein cows were randomly assigned into a replicated 3 × 3 Latin square design trial. Three treatments were control diet (CON, 20% dietary starch, DM basis, high grain diet (HG, 33.2% dietary starch, DM basis and high grain diet supplemented with 180 mg thiamine/kg DMI (HG+T. On day 21 of each period, rumen content samples were collected at 3 h postfeeding. Ruminal thiamine concentration was detected by high performance liquid chromatography. The microbiota composition was determined using Illumina MiSeq sequencing of 16S rRNA gene. Cows receiving thiamine supplementation had greater ruminal pH value, acetate and thiamine content in the rumen. Principal coordinate analysis and similarity analysis indicated that HG feeding and thiamine supplementation caused a strong shift in bacterial composition and structure in the rumen. At the genus level, compared with CON group, the relative abundances of 19 genera were significantly changed by HG feeding. Thiamine supplementation increased the abundance of cellulolytic bacteria including Bacteroides, Ruminococcus 1, Pyramidobacter, Succinivibrio, and Ruminobacter, and their increases enhanced the fiber degradation and ruminal acetate production in HG+T group. Christensenellaceae R7, Lachnospira, Succiniclasticum, and Ruminococcaceae NK4A214 exhibited a negative response to thiamine supplementation. Moreover, correlation analysis revealed that ruminal thiamine concentration was

  13. The effects of coarse ground corn, whole sorghum, and a prebiotic on growth performance, nutrient digestibility, and cecal microbial populations in broilers fed diets with and without corn distillers dried grains with solubles.

    Science.gov (United States)

    Jacobs, China; Parsons, Carl M

    2013-09-01

    Two experiments were conducted from 0 to 21 d of age and evaluated diets containing combinations of fine or coarse ground corn (557 or 1,387 μm, respectively), whole sorghum, 15% corn distillers dried grains with solubles (DDGS), or a prebiotic-type product containing yeast cell wall, lactose, citric acid, and other fermentable carbohydrates. In experiment 1, feed efficiency was decreased (P whole sorghum, whereas broilers receiving diets with 15% DDGS had increased feed efficiency (P whole sorghum diets had increased (P whole sorghum in combination with DDGS can be fed to broilers with no long-term adverse effects on growth performance and nutrient digestibility and that these ingredients can have beneficial effects on AMEn, gizzard size, and cecal microflora in some instances.

  14. Comparative feeding value of distillers dried grains plus solubles as a partial replacement for steam-flaked corn in diets for calf-fed Holstein steers: characteristics of digestion, growth performance, and dietary energetics.

    Science.gov (United States)

    Carrasco, R; Arrizon, A A; Plascencia, A; Torrentera, N G; Zinn, R A

    2013-04-01

    Two experiments were conducted to examine the effect of level of dried distillers grains plus solubles (DDGS) supplementation (0, 10, 20, and 30%; DM basis), replacing steam-flaked (SF) corn in finishing diets, on characteristics of digestion (Exp. 1) and growth performance (Exp. 2) in calf-fed Holstein steers. In Exp.1, 4 cannulated Holstein steers (349 ± 12 kg) were used to evaluate treatment effects on characteristics of digestion. Ruminal NDF digestion tended to increase (quadratic effect, P = 0.09) and ruminal OM digestion decreased (linear effect, P = 0.01) with DDGS substitution. There were no treatment effects on duodenal flow of microbial N (MN). Substitution with DDGS increased (linear effect, P feeding period), treatment effects on ADG and G:F were small (P ≥ 0.22). Compared with the other treatments, HCW was greater (3.4; P = 0.03) at the 20% level of DDGS substitution. The NE value for DDGS in SF corn-based diets for the calf-fed Holstein are consistent with current tabular standards. Extra-caloric value of DDGS as a metabolizable AA source is apparent during the initial growing phase. The UIP value of DDGS used in this study (35%) was considerably less than current tabular estimates (52%; NRC, 2000).

  15. Brewer spectrometer total ozone column measurements in Sodankylä

    Science.gov (United States)

    Karppinen, Tomi; Lakkala, Kaisa; Karhu, Juha M.; Heikkinen, Pauli; Kivi, Rigel; Kyrö, Esko

    2016-06-01

    Brewer total ozone column measurements started in Sodankylä in May 1988, 9 months after the signing of The Montreal Protocol. The Brewer instrument has been well maintained and frequently calibrated since then to produce a high-quality ozone time series now spanning more than 25 years. The data have now been uniformly reprocessed between 1988 and 2014. The quality of the data has been assured by automatic data rejection rules as well as by manual checking. Daily mean values calculated from the highest-quality direct sun measurements are available 77 % of time with up to 75 measurements per day on clear days. Zenith sky measurements fill another 14 % of the time series and winter months are sparsely covered by moon measurements. The time series provides information to survey the evolution of Arctic ozone layer and can be used as a reference point for assessing other total ozone column measurement practices.

  16. Ronnie Brewer 快红新生代9

    Institute of Scientific and Technical Information of China (English)

    Husky

    2008-01-01

    经过一年在板凳上见习观摩,以及季前暑假努力自我加强之后,爵士队在2006年选进的布鲁尔(Ronnie Brewer )本季一开始就脱胎换骨大跃进,成为得分后卫固定先发。

  17. Brewer's Yeast Improves Blood Pressure in Type 2 Diabetes Mellitus

    Directory of Open Access Journals (Sweden)

    Payam Hosseinzadeh

    2013-06-01

    Full Text Available Background: This study was conducted to investigate the effects of Brewer's yeast supplementation on serum lipoproteins and blood pressure in patients with Type 2 diabetes mellitus.Methods: In a randomized double blind clinical trial, 90 adults with type 2 diabetes mellitus were recruited, and divided randomly into 2 groups, trial group received brewer's yeast (1800 mg/day and control group received placebo for 12 weeks. Weight, BMI, food consumption (based on 24 hour food recall, fasting serum lipoproteins (Cholesterol, Triglyceride, LDL-c, HDL-c, systolic and diastolic blood pressures were measured before and after the intervention. Data analyses were performed by Statistical Package for Social Sciences ver. 18.0, and the statistical tests included Independent t-test, Paired t-test, Kolmogorov-Smirnov and analysis of covariance. This trial was registered in Iranian Registry of Clinical Trials (IRCT, No.IRCT138807062513N1.Results: Eighty-four subjects (21 men and 63 women aged 46.3±6.1 years completed the study. After 12 weeks supplementation, systolic and diastolic blood pressures were decreased in the group receiving brewer's yeast (4.1±1.5, P=0.007 and 5.7±0.6, P=0.001 respectively. No-significant changes in LDL-c, HDL-c, Triglyceride and Cholesterol were shown.Conclusion: Supplementation with Brewer's yeast besides the usual treatment of type 2 diabetes mellitus can reduce systolic and diastolic blood pressures in diabetic patients.

  18. Validation of Brewer and Pandora measurements using OMI total ozone

    Science.gov (United States)

    Baek, Kanghyun; Kim, Jae H.; Herman, Jay R.; Haffner, David P.; Kim, Jhoon

    2017-07-01

    Korea will launch the Geostationary Environment Monitoring Spectrometer (GEMS) instrument in 2018 onboard the Geostationary Korean Multi-Purpose Satellite to monitor tropospheric gas concentrations with high temporal and spatial resolutions. The purpose of this study is to examine the performance of total column ozone (TCO) measurements from ground-based Pandora and Brewer instruments that will be used for validation of the GEMS ozone product. Satellite measurements can be used to detect erroneous outliers at a particular ground station, which deviate significantly from co-located satellite measurements relative to other stations. This is possible because a single satellite retrieval algorithm is used to process the entire satellite dataset, and instrument characteristics typically change slowly over the life of the satellite. Thus, the short-term stability (months) of satellite measurements can be used to estimate the performance of the ground-based measurement network as well as to identify potential problems at individual stations. As a reference for satellite ozone measurements, we have selected TCO data derived from OMI-TOMS V8.5 algorithm, because it is a robust algorithm that has been well studied to identify its various error sources. We validated ground-based Brewer and Pandora TCO measurements using OMI-TOMS TCO data collected over South Korea from March 2012 to December 2014. The Brewer TCO measurements at Pohang showed significant deviation from overall seasonal variation during the study period. In addition, in the presence of clouds, Pandora TCO measurements are unusually ∼7% higher than OMI-TOMS TCO data. To filter out these cloud-contaminated data, we applied a Kalman filter to the Pandora measurements. The diurnal variation in the Kalman-filtered Pandora data agrees well with the Brewer data, and the correlation of Kalman-filtered Pandora data with OMI-TOMS TCO is significantly improved from 0.89 to 0.99 at Seoul and from 0.93 to 0.99 at Busan.

  19. Investigation of zinc biosorption by brewer's yeast cells

    Directory of Open Access Journals (Sweden)

    Dodić Siniša N.

    2005-01-01

    Full Text Available The highest amount of zinc (= 90% is bound after 3 hrs of contact at low initial (total concentrations of zinc in suspension of yeast, 10-100 mg/l at 10-30°C. The equilibrium between bound and free zinc ions is established after 6 hrs of contact time, independently on the total zinc concentration in yeast milk. No bigger changes of content of zinc bound to brewer's yeast cells was determined at temperatures 10°C and 30°C. 40% of bound zinc in the equilibrium state is bound during the first 15 min of contact of zinc ions and brewer's yeast cells at all initial (total zinc concentrations in suspension of yeast both at 10°C and 30°C. The "KEKAM" equation can be used for the description of kinetics of zinc biosorption by waste brewer's yeast cells, for the ranges of zinc concentration 10-100 mg/l at 30°C (mean correlation coefficient 0,96 and 60,0-100 mg/l at 10°C (mean correlation coefficient 0,95.

  20. 27 CFR 25.152 - Reduced rate of tax for certain brewers.

    Science.gov (United States)

    2010-04-01

    ... TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Tax on Beer Liability for Tax § 25.152 Reduced... reduced rate of tax on the first 60,000 barrels of beer removed for consumption or sale by a brewer during a calendar year. To be eligible to pay the reduced rate of tax, a brewer: (1) Shall brew or produce...

  1. Effect of Supplemental Corn Dried Distillers Grains with Solubles Fed to Beef Steers Grazing Native Rangeland during the Forage Dormant Season

    Science.gov (United States)

    Murillo, M.; Herrera, E.; Ruiz, O.; Reyes, O.; Carrete, F. O.; Gutierrez, H.

    2016-01-01

    Two experiments were conducted to evaluate the effects of the level of corn dry distillers grains with solubles (CDDGS) supplementation on growing performance, blood metabolites, digestion characteristics and ruminal fermentation patterns in steers grazing dormant forage. In Exp. 1, of growth performance, 120 steers (204±5 kg initial body weight [BW]) were distributed randomly into 3 groups (each of 40 steers), which were provided with the following levels of CDDGS supplement: 0%, 0.25%, or 0.50% BW. All groups of steers were grazed for 30 days in each of 3 grazing periods (March, April, and May). Approximately 1,000 ha of the land was divided with electric fencing into 3 equally sized pastures (333 ha in size). Blood samples were collected monthly from 20 steers in each grazing group for analysis of glucose (G), urea-nitrogen (UN) and non-esterified fatty acids. Final BW, average daily gain (ADG) and supplement conversion (CDDGS-C) increased with increasing levels of CDDGS supplementation (p<0.05).The CDDGS supplementation also increased the plasma G and UN concentrations (p<0.05). In Exp. 2, of digestive metabolism, 9 ruminally cannulated steers (BW = 350±3 kg) were distributed, following a completely randomized design, into groups of three in each pasture. The ruminally cannulated steers were provided the same levels of CDDGS supplementation as in the growing performance study (0%, 0.25%, and 0.50% BW), and they grazed along with the other 40 steers throughout the grazing periods. The dry matter intake, crude protein intake, neutral detergent fiber intake (NDFI), apparent digestibility of dry matter (ADDM), crude protein (ADCP) and neutral detergent fiber (ADNDF) increased with increasing levels of CDDGS supplementation (p<0.05). The ruminal degradation rates of CP (kdCP), NDF (kdNDF) and passage rate (kp) also increased with increasing levels of CDDGS supplementation (p<0.05). Ruminal ammonia nitrogen (NH3-N) and propionate concentrations also increased with

  2. Resolution of brewers' yeast pyruvate decarboxylase into two isozymes.

    Science.gov (United States)

    Kuo, D J; Dikdan, G; Jordan, F

    1986-03-01

    A novel purification method was developed for brewers' yeast pyruvate decarboxylase (EC 4.1.1.1) that for the first time resolved the enzyme into two isozymes on DEAE-Sephadex chromatography. The isozymes were found to be distinct according to sodium dodecyl sulfate polyacrylamide gel electrophoresis: the first one to be eluted gave rise to one band, the second to two bands. The isozymes were virtually the same so far as specific activity, KM, inhibition kinetics and irreversible binding properties by the mechanism-based inhibitor (E)-4-(4-chlorophenyl)-2-oxo-3-butenoic acid are concerned. This finding resolves a longstanding controversy concerning the quaternary structure of this enzyme.

  3. Closure of the Brewer Gold Mine by pit backfilling

    Energy Technology Data Exchange (ETDEWEB)

    Lewis-Russ, A.; Lupo, J.F. [Titan Environmental Corp., Englewood, CO (United States); Bronson, J.M. [Titan Environmental Corp., Tempe, AZ (United States)] [and others

    1996-12-31

    Brewer Gold Mine, located in north-central South Carolina, is implementing an innovative reclamation plan that includes backfilling the main Brewer open pit with mine waste. The primary goals of the closure are to reduce acid rock drainage and minimize or eliminate long-term operation and maintenance requirements by restoring the site property to approximate pre-mining topography. The plan calls for consolidation of approximately 200 acres of waste into approximately 20 hectares (50 acres). Much of the material to be backfilled into the pit, including spent heap leach material and waste rock, has acid-generating potential. Therefore, the backfill design integrated geochemical properties of the backfill materials with expected post-closure conditions. A prime consideration was the final position of the water table. Since mining at the site started in the early 1800`s, no records exist of the original groundwater levels. Therefore, the design incorporates a large anoxic limestone drain to control the final groundwater level. Additional amendments are to be placed in targeted areas of the backfill to maximize their utilization. A low-permeability cap system that includes a GEOSYNTHETIC clay liner has been designed to limit infiltration into the backfill.

  4. Intercomparison of aerosol optical depth from Brewer ozone spectrophotometers and CIMEL sunphotometers measurements

    Directory of Open Access Journals (Sweden)

    A. Cheymol

    2008-06-01

    Full Text Available The Langley plot method applied on the Brewer Ozone measurements can provide accurate Aerosol Optical Depth (AOD in the UV-B. We present seven intercomparisons between AOD retrieved from Brewer Ozone measurements and AOD measured by CIMEL sunphotometer, which are stored in the international AERONET database. Only the intercomparisons between co-located instruments can be used to validate the Langley Plot method applied to the Brewer measurements: in this case, all the correlation coefficient are above 0.83. If the instruments are not at the same site, the correlation between the AOD retrieved by both instruments is much lower.

  5. Desempenho e digestibilidade in vivo de cordeiros alimentados com dietas contendo canola em grão integral em diferentes formas Performance and digestibility in vivo of lambs fed diets with whole canola grain in different forms

    Directory of Open Access Journals (Sweden)

    Vanderlei Bett

    1999-08-01

    Full Text Available O desempenho e a digestibilidade dos nutrientes de cordeiros alimentados com concentrados formulados com farelo de soja (FS, canola integral (CI canola quebrada (CQ ou canola peletizada (CP e feno de aveia, fornecidos na relação 30/70 (volumoso/conconcentrado, %MS foram avaliados. Vinte oito cordeiros machos com idade inicial entre 60 e 90 dias e 17 kg PV foram distribuídos em delineamento inteiramente casualizado. As ingestões (g/d de MS, PB, FDA, FDN e EB (Mcal/dia, o ganho médio diário e a conversão alimentar, foram semelhantes. Não houve diferenças para digestibilidade aparente da MS, PB e EB, exceto para digestibilidade de FDN (46,84; 60,11; 50,10; e 38,88% e FDA (45,84; 54,19; 46,57; e 29,59% para FS, CI, CQ e CP, respectivamente. Houve menor retenção de nitrogênio para CP (3,0 g/d em comparação às outras dietas (entre 5,0 e 7,3 g/d. Os tratamentos não diferiram na concentração de propionato, mas reduziram as concentrações de butirato (7,08; 4,87; 4,08; e 4,29 μM/mL de líquido ruminal e N-amoniacal (12,17; 8,69; 8,40; e 7,66 mg/100 mL de líquido de rúmen. O uso de canola, nas diferentes formas, não influenciou a ingestão e a digestão, proporcionando desempenho semelhante entre os tratamentos.The performance and digestibility of nutrients of lambs fed concentrates formulated with soybean meal (SM and whole canola grain (WC, cracked canola grain (CC or pelleted canola (PC and oat hay, fed in a 30:70 (forage to concentrate ratio, %DM were evaluated. Twenty-eight male lambs with initial age from 60 to 90 days and 17 kg LW were allotted to a completely randomized design. The intakes (g/d of DM, CP, ADF, NDF and GE (Mcal/d, the average daily gain and feed: gain ratio, were similar. There were no differences for apparent digestibilities of MS, PB and GE, except for the NDF digestibility (46.84, 60.11, 50.10, and 38.88% and ADF (45.84, 54.19, 46.57, and 29.59% for SM, WC, CC and PC, respectively. There was lower nitrogen

  6. Effects of brewers` condensed solubles (BCS) on the production of ethanol from low-grade starch materials

    Energy Technology Data Exchange (ETDEWEB)

    Choi, C.H.; Chung, D.S.; Seib, P.A. [Kansas State Univ., Manhattan, KS (United States)] [and others

    1995-02-01

    Yeast fermentation was performed on grain and bakery byproducts with and without adding the same volume of brewers` condensed solubles (BCS). Starch material in the grain and bakery byproducts effectively was converted to fermentable sugars with conversion ratios of 93-97% by successive treatments of samples with bacterial {alpha}-amylase and fungal glucoamylase. The yeast fermentation of these enzyme-digested byproducts alone showed that ethanol concentrations of 16.4-42.7 mL/100 g dry solid in the broth were achieved with fermentation efficiencies of 87-96%. Addition of BCS to the grain byproducts increased ethanol concentration by 10-86% by increasing the potential glucose content of the broth. The rates of fermentation measured by CO{sub 2} gas production demonstrated that BCS addition to bakery byproducts reduced the fermentation time from 62-72 h to 34-35 h. In bakery byproducts that were low in amino nitrogen, exhaustion of nitrogenous compounds in substrates was found to be a limiting factor for yeast growth. Because BCS is a rich source of nitrogen, adding BCS to these substrates markedly increased the fermentation rate. 15 refs., 4 figs., 3 tabs.

  7. Recombinant brewer's yeast strains suitable for accelerated brewing.

    Science.gov (United States)

    Suihko, M L; Blomqvist, K; Penttilä, M; Gisler, R; Knowles, J

    1990-06-01

    Four brewer's yeast strains carrying the alpha-ald gene of Klebsiella terrigena (ex. Aerobacter aerogenes) or of Enterobacter aerogenes on autonomously replicating plasmids were constructed. The alpha-ald genes were linked either to the ADC1 promoter or to the PGK1 promoter of yeast Saccharomyces cerevisiae. In pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. All other brewing properties of the strains were unaffected and the quality of finished beers was as good as that of finished beer prepared with the control strain. The total process time of beer production could therefore be reduced to 2 weeks, in contrast to about 5 weeks required in the conventional process.

  8. Genome annotation of a Saccharomyces sp. lager brewer's yeast

    Directory of Open Access Journals (Sweden)

    Patricia Marcela De León-Medina

    2016-09-01

    Full Text Available The genome of lager brewer's yeast is a hybrid, with Saccharomyces eubayanus and Saccharomyces cerevisiae as sub-genomes. Due to their specific use in the beer industry, relatively little information is available. The genome of brewing yeast was sequenced and annotated in this study. We obtained a genome size of 22.7 Mbp that consisted of 133 scaffolds, with 65 scaffolds larger than 10 kbp. With respect to the annotation, 9939 genes were obtained, and when they were submitted to a local alignment, we found that 53.93% of these genes corresponded to S. cerevisiae, while another 42.86% originated from S. eubayanus. Our results confirm that our strain is a hybrid of at least two different genomes.

  9. Evaluating biochemical methane production from brewer's spent yeast.

    Science.gov (United States)

    Sosa-Hernández, Ornella; Parameswaran, Prathap; Alemán-Nava, Gibrán Sidney; Torres, César I; Parra-Saldívar, Roberto

    2016-09-01

    Anaerobic digestion treatment of brewer's spent yeast (SY) is a viable option for bioenergy capture. The biochemical methane potential (BMP) assay was performed with three different samples (SY1, SY2, and SY3) and SY1 dilutions (75, 50, and 25 % on a v/v basis). Gompertz-equation parameters denoted slow degradability of SY1 with methane production rates of 14.59-4.63 mL/day and lag phases of 10.72-19.7 days. Performance and kinetic parameters were obtained with the Gompertz equation and the first-order hydrolysis model with SY2 and SY3 diluted 25 % and SY1 50 %. A SY2 25 % gave a 17 % of TCOD conversion to methane as well as shorter lag phase (methane production. Methane capture and biogas composition were dependent upon the SY source, and co-digestion (or dilution) can be advantageous.

  10. Brewers dried yeast as a source of mannan oligosaccharides for weanling pigs.

    Science.gov (United States)

    White, L A; Newman, M C; Cromwell, G L; Lindemann, M D

    2002-10-01

    Brewers dried yeast, a source of mannan oligosaccharides (MOS), was assessed as an alternative to an antimicrobial agent (carbadox) for young pigs in two experiments. The yeast contained 5.2% MOS. Agglutination tests confirmed adsorption of several serovars of E. coli and Salmonella spp. onto the yeast product. In Exp. 1, seven replicates (five pigs per pen) of 22-d-old pigs were fed a nonmedicated basal diet or the basal diet with carbadox (55 mg/kg), yeast (3%), or a combination of 3% yeast and 2% citric acid for 28 d. Carbadox did not improve growth performance. Growth rate and feed intake were depressed (P yeast alone or in combination with acid. Log counts of total coliforms, Escherichia coli, and Clostridium perfringens in feces were not affected by diet, but Bifidobacteria spp. counts were lower (P yeast + acid diet and lactobacilli counts were higher (P yeast. Fecal pH and VFA concentrations and intestinal morphological traits were not consistently affected by diet. Serum IgG levels were elevated in the yeast + acid (P yeast and carbadox additions to the diet on enteric microbial populations in young pigs housed in isolation units were evaluated. Pigs (n = 24) were weaned at 11 d of age (4.1 kg BW) and placed in isolation chambers (two pigs per chamber) equipped with individual air filtering systems and excrement containers. Treatments were a nonmedicated basal diet and the basal diet with 55 mg/kg of carbadox or with 3% yeast. Diets were fed for 29 d, then each pig was orally dosed with approximately 9.5 x 10(8) CFU of E. coli K88. Daily fecal E. coli K88 counts were not different (P > 0.05) among treatments, but fecal shedding of carbadox-resistant coliforms was higher (P yeast (P Yeast reduced colonization oftotal coliforms in the duodenum,jejunum, cecum, and colon, but it did not have a consistent effect on colonization of E. coli K88. Pigs fed yeast tended (P yeast and carbadox had minimal effects on growth, microbial populations, and intestinal health

  11. Economic Viability of Brewery Spent Grain as a Biofuel

    Energy Technology Data Exchange (ETDEWEB)

    Morrow, Charles [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2016-01-01

    This report summarizes an investigation into the technical feasibility and economic viability of use grain wastes from the beer brewing process as fuel to generate the heat needed in subsequent brewing process. The study finds that while use of spent grain as a biofuel is technically feasible, the economics are not attractive. Economic viability is limited by the underuse of capital equipment. The investment in heating equipment requires a higher utilization that the client brewer currently anticipates. It may be possible in the future that changing factors may swing the decision to a more positive one.

  12. Biogas Production from Brewer's Yeast Using an Anaerobic Sequencing Batch Reactor.

    Science.gov (United States)

    Zupančič, Gregor Drago; Panjičko, Mario; Zelić, Bruno

    2017-06-01

    Renewable energy sources are becoming increasingly important in the beverage and food industries. In the brewing industry, a significant percentage of the used raw materials finishes the process as secondary resource or waste. The research on the anaerobic digestion of brewer's yeast has been scarce until recent years. One of the reasons for this is its use as a secondary resource in the food industry and as cattle feed. Additionally, market value of brewer's yeast is higher than its energy value. Due to the increase of energy prices, brewer's yeast has become of interest as energy substrate despite its difficult degradability in anaerobic conditions. The anaerobic co-digestion of brewer's yeast and anaerobically treated brewery wastewater was studied using a pilot-scale anaerobic sequencing batch reactor (ASBR) seeded with granular biomass. The experiments showed very good and stable operation with an organic loading rate of up to 8.0 kg/(m(3)·day), and with a maximum achieved organic loading rate of 13.6 kg/(m(3)·day) in a single cycle. A specific biogas productivity of over 0.430 m(3)/kg of the total chemical oxygen demand (COD) inserted, and total COD removal efficiencies of over 90% were achieved. This study suggests that the brewer's yeast can be successfully digested in an ASBR without adverse effects on the biogas production from brewer's yeast/wastewater mixtures of up to 8% (by volume). By using the brewer's yeast in the ASBR process, the biogas production from brewery wastewater could be increased by 50%.

  13. Ozone, spectral irradiance and aerosol measurements with the Brewer spectro radiometer; Misure di ozono, irradianza spettrale ultravioletta e aerosol con lo spettroradiometro Brewer

    Energy Technology Data Exchange (ETDEWEB)

    Marenco, F.; Di Sarra, A. [ENEA, Centro Ricerche Casaccia, Rome (Italy)

    2001-07-01

    In this technical report a detailed description of the Brewer spectro radiometer, a widespread instrument for ozone and ultraviolet radiation, is given. The methodologies used to measure these quantities and for instrument calibration are described in detail. Finally a new methodology, developed by ENEA to derive the aerosol optical depth from the Brewer routine total ozone measurements, is described. This methodology is based on Langley extrapolation, on the determination of the transmissivity of the Brewer neutral density filters, and on a statistically significant number of half days of measurements obtained in could-free conditions. Results of this method, obtained with the Brewer of the ENEA station for climate observations Roberto Sarao, located in the island of Lampedusa, are reported. These results confirm the validity of the method, thanks to independent measurements taken in 1999 with a Multi filter Rotating Shadow band Radiometer. This methodology allows researchers to obtain an aerosol climatology from ozone measurements obtained at several sites world-wide. [Italian] In questo rapporto tecnico viene fornita la descrizione dettagliata di uno strumento comunemente utilizzato per le misure di ozono e radiazione ultravioletta: lo spettroradiometro Brewer. Le metodologie usate per la misura di queste grandezze e per la calibrazione dello strumento vengono descritte in dettaglio. Infine, viene descritto una nuova metodologia, messa a punto dall'ENEA, per ricavare lo spessore ottico degli aerosol a partire dalle misure di ozono fatte normalmente dal Brewer. Questa metodologia si basa su di una calibrazione effettuata con il metodo dell' estrapolazione di Langley, sulla misura della trasmissivita' dei filtri a densita' neutra dello strumento, e su un numero statisticamente grande di mezze giornate di misure effettuate in assenza di nuvole. Sono riportati alcuni risultati della metodologia, ottenuti con il Brewer della Stazione per le

  14. Desempenho de bezerros holandeses alimentados até o desaleitamento com silagem de grãos úmidos ou grãos secos de milho ou sorgo Performance of Holstein calves fed until weaning time with high moisture grains silage or dry ground grains of corn or sorghum

    Directory of Open Access Journals (Sweden)

    Gercílio Alves de Almeida Júnior

    2008-01-01

    Full Text Available Avaliou-se o desempenho de bezerros alimentados até o desaleitamento com silagem de grãos úmidos ou grãos secos de milho ou sorgo para posterior produção de vitelos de carne rosa. Trinta bezerros holandeses foram distribuídos em delineamento em blocos casualizados, com cinco blocos e seis tratamentos, e alimentados com seis rações concentradas com teores similares de proteína (22,5% PB e de energia (3,2 Mcal EM/kg MS, formuladas com: milho seco moído (MM; silagem de grãos úmidos de milho (SGUM; sorgo seco com tanino moído (SCTM; silagem de grãos úmidos de sorgo com tanino (SGUSCT; sorgo seco sem tanino moído (SSTM; e silagem de grãos úmidos de sorgo sem tanino (SGUSST. Os animais receberam sucedâneo de leite até atingirem o peso pré-estabelecido para desaleitamento (60 ± 5 kg. Não houve efeito das rações concentradas sobre a altura de cernelha, a idade ao desaleitamento, os consumos diários de MS diários e totais das rações concentradas, os ganhos de peso diários e totais e a conversão alimentar. Os custos por kg de ganho de peso das rações concentradas contendo sorgo foram mais baixos que o das rações concentradas formuladas com milho. Todos os alimentos avaliados podem ser usados em rações concentradas para bezerros em fase de aleitamento, pois não comprometem o desempenho e conferem resultados similares.The performance of calves fed until weaning time with high moisture grains silage or dry ground grains of corn or sorghum was evaluated, for posterior production of pink meat veal. Thirty Holstein calves were allotted to a complete randomized blocks experimental design with five blocks and six treatments, fed with six concentrate rations with similar contents of protein (22.5% CP and energy (3.2 Mcal ME/kg DM formulated with: dry ground corn (GC, high moisture corn silage (HMCS, dry ground sorghum with tannin (GSWT, high moisture sorghum with tannin silage (HMSWTS, dry ground sorghum without tannin (GSWTT

  15. Subunit structure of 6-phosphofructokinase from brewers' yeast.

    Science.gov (United States)

    Tamaki, N; Hess, B

    1975-11-01

    An analysis of 6-phosphofructokinase from brewers' yeast in the presence of sodium dodecylsulfate reveals the occurrence of four components with the following molecular weights: alpha = 140000, beta = 130000, and alpha' = 92000, beta' = 87000. It was found that the alpha- and beta-components can be converted to the alpha' and beta' components by treatment of the native preparation with hyaluronidase. A comparison of the molecular weight obtained by ultracentrifugation and gel filtration with the results obtained by dodecylsulfate electrophoresis after treatment with hyaluronidase reveals that the alpha' and beta' components are the smallest molecular structures obtained upon dissociation of the native enzyme. The mechanism of action of hyaluronidase suggests a desensitization of the alpha and beta components of the enzyme towards dodecylsulfate. Thus, in the absence of hyaluronidase treatment; only an apparent molecular weight for the alpha and beta component is obtained. The analysis indicates that the native enzyme might be composed of four different subunits with an alpha, beta, alpha' and beta' configuration. It is not excluded that the native enzyme consists only of alpha- and beta-chains.

  16. Valuation of brewers spent yeast polysaccharides: a structural characterization approach.

    Science.gov (United States)

    Pinto, Mariana; Coelho, Elisabete; Nunes, Alexandra; Brandão, Tiago; Coimbra, Manuel A

    2015-02-13

    Brewers spent yeast (BSY) is a by-product from beer industry that can be exploited as source of glucans and mannoproteins, with potential biological activities. In order to solubilize these carbohydrate-rich polymeric materials, a sequential extraction with hot water and alkaline solutions (0.1-8 M KOH) was performed. Mannoproteins were mainly (85%) extracted with 4 M KOH whereas glucans were extracted with 8 M KOH and in an amount that accounted only for 34% of total glucose. Final residue still accounted for 34% of the initial glucans and contained 98% of glucose. Cellulase and α-amylase treatments showed the presence of both α- and β-(1→4)-Glc linkages. To promote total solubilization of these insoluble glucans, the final residue was submitted to a partial acid hydrolysis. This work is the first report showing that the most abundant polysaccharides in BSY are polymers that contain structural features similar to cellulose, thus justifying their resistance to alkaline extractions, acid hydrolysis, and insolubility in water.

  17. Climate Change and the Brewer-Dobson Circulation

    Science.gov (United States)

    Rind, David

    1999-01-01

    Using GCM (General Circulation Model) simulations of the troposphere-stratosphere circulation in different climate regimes, the sensitivity of the Brewer-Dobson Circulation to climate change will be discussed. Included will be simulations of the stratosphere with increasing/doubled CO2 as done with different versions of the GISS (Goddard Institute for Space Studies) Global Climate Middle Atmosphere Model, with and without ozone response. To put the results in a broader perspective, stratospheric circulation changes from several paleoclimate simulations will also be presented, including the Last Glacial Maximum and the Paleocene, each with several different (plausible) latitudinal sea surface temperature gradients. Hence the climate changes investigated will include strong variations in baroclinic and topographic tropospheric planetary wave and gravity wave forcing, as well as global mean temperature and atmospheric CO2 levels. Results will be analyzed in terms of wave driving via E-P flux convergences and gravity wave effects, and will be shown to be strongly dependent on wave propagation characteristics.

  18. Beer brewing using a fusant between a sake yeast and a brewer's yeast.

    Science.gov (United States)

    Mukai, N; Nishimori, C; Fujishige, I W; Mizuno, A; Takahashi, T; Sato, K

    2001-01-01

    Beer brewing using a fusant between a sake yeast (a lysine auxotrophic mutant of sake yeast K-14) and a brewer's yeast (a respiratory-deficient mutant of the top fermentation yeast NCYC1333) was performed to take advantage of the beneficial characteristics of sake yeasts, i.e., the high productivity of esters, high tolerance to ethanol, and high osmotolerance. The fusant (F-32) obtained was different from the parental yeasts regarding, for example, the assimilation of carbon sources and tolerance to ethanol. A brewing trial with the fusant was carried out using a 100-l pilot-scale plant. The fusant fermented wort more rapidly than the parental brewer's yeast. However, the sedimentation capacity of the fusant was relatively low. The beer brewed using the fusant contained more ethanol and esters compared to that brewed using the parental brewer's yeast. The fusant also obtained osmotolerance in the fermentation of maltose and fermented high-gravity wort well.

  19. Spectral ultraviolet measurements by a multichannel monitor and a brewer spectroradiometer: a field study.

    Science.gov (United States)

    Di Menno, I; Moriconi, M L; Di Menno, M; Casale, G R; Siani, A M

    2002-01-01

    Two different instruments for measuring the spectral UV irradiance were used in a field comparison study in July 2000 in Rome, Italy: a Brewer spectrophotometer and a moderate-bandwidth filter radiometer (GUV-511C). The Brewer is designed to measure the solar spectral irradiances in the region from 290 nm to 325 nm with a spectral resolution of 0.5 nm. The GUV-511C measures hand-averaged spectral irradiance at four wavelengths: 305, 320, 340 and 380 nm with a bandwidth depending on the filter type for each channel (about 10 nm full width half maximum, FWHM). Comparisons between the two instruments were made for 5 days for the two wavelengths 305 and 320 nm under different meteorological conditions with the Brewer taken as the reference.

  20. Intercomparison of Aerosol Optical Depth from Brewer Ozone spectrophotometers and CIMEL sunphotometers measurements

    Directory of Open Access Journals (Sweden)

    A. Cheymol

    2009-01-01

    Full Text Available The Langley plot method applied on the Brewer Ozone measurements can provide accurate Aerosol Optical Depth (AOD in the UV-B. We present seven intercomparisons between AOD retrieved from Brewer Ozone measurements at 320 nm and AOD measured by CIMEL sunphotometer at 340 nm or 440 nm (shifted to 320 nm in using the Angström's law, which are stored in the international AERONET database. Only the intercomparisons between co-located instruments can be used to validate the Langley Plot Method applied to the Brewer measurements: in this case, all the correlation coefficients are above 0.82. If the instruments are not at the same site, the correlation between the AOD retrieved by both instruments is much lower. In applying the Angström's law the intercomparison is improved compared to previous study.

  1. Effect of Brewer's Yeast-Induced Pyrexia on Aminophylline-Elicited Convulsions in Mice

    Directory of Open Access Journals (Sweden)

    Araki,Hiroaki

    2009-10-01

    Full Text Available Theophylline-associated convulsions have been observed most frequently in children with fever, but the mechanism is not fully understood. In this study, we investigated the basic mechanism of aminophylline [theophylline-2-ethylenediamine]-induced convulsions and the effects of Brewer's yeast-induced pyrexia in mice. Diazepam (5-10mg/kg, i.p., a benzodiazepine receptor agonist, significantly prolonged the onset and significantly decreased the incidence of convulsions induced by aminophylline (350mg/kg, i.p.. However, the gamma aminobutyric acid (GABAA receptor agonist muscimol (1-4mg/kg, i.p., the GABAB receptor agonist baclofen (2-4mg/kg, i.p. and the N-methyl-D-aspartic acid receptor antagonist dizocilpine (0.1-0.3mg/kg, i.p. failed to protect against the convulsions. 20% Brewer's yeast (0.02ml/g, s.c. increased body temperature by 1.03, and also significantly shortened the onset and significantly increased the incidence of convulsions induced by aminophylline. The anticonvulsant action of diazepam (2.5-10mg/kg, i.p. on the convulsions induced by aminophylline was reduced by Brewer's yeast-induced pyrexia. The proconvulsant actions of the GABAA receptor antagonists picrotoxin (3-4mg/kg, i.p. and pentylenetetrazol (40-60mg/kg, i.p. were enhanced by Brewer's yeast. These results suggest that the anticonvulsant action of diazepam against aminophylline is reduced by Brewer's yeast-induced pyrexia, and that GABAA receptors are involved in the aggravation of the convulsions by Brewer's yeast in mice.

  2. Influence of the ozone profile above Madrid (Spain) on Brewer estimation of ozone air mass factor

    Energy Technology Data Exchange (ETDEWEB)

    Anton, M. [Univ. de Extremadura, Badajoz (Spain). Dept. de Fisica; Evora Univ. (PT). Goephysics Centre of Evora (CGE); Lopez, M.; Banon, M. [Agenica Estatal de Meteorologia (AEMET), Madrid (Spain); Costa, M.J.; Silva, A.M. [Evora Univ. (PT). Goephysics Centre of Evora (CGE); Evora Univ. (Portugal). Dept. of Physics; Serrano, A. [Univ. de Extremadura, Badajoz (Spain). Dept. de Fisica; Bortoli, D. [Evora Univ. (PT). Goephysics Centre of Evora (CGE); Vilaplana, J.M. [Instituto Nacional de Tecnica Aeroespacial (INTA), Huelva (Spain). Estacion de Sondeos Atmosferico ' ' El Arenosillo' '

    2009-07-01

    The methodology used by Brewer spectroradiometers to estimate the ozone column is based on differential absorption spectroscopy. This methodology employs the ozone air mass factor (AMF) to derive the total ozone column from the slant path ozone amount. For the calculating the ozone AMF, the Brewer algorithm assumes that the ozone layer is located at a fixed height of 22 km. However, for a real specific site the ozone presents a certain profile, which varies spatially and temporally depending on the latitude, altitude and dynamical conditions of the atmosphere above the site of measurements. In this sense, this work address the reliability of the mentioned assumption and analyses the influence of the ozone profiles measured above Madrid (Spain) in the ozone AMF calculations. The approximated ozone AMF used by the Brewer algorithm is compared with simulations obtained using the libRadtran radiative transfer model code. The results show an excellent agreement between the simulated and the approximated AMF values for solar zenith angle lower than 75 . In addition, the relative differences remain lower than 2% at 85 . These good results are mainly due to the fact that the altitude of the ozone layer assumed constant by the Brewer algorithm for all latitudes notably can be considered representative of the real profile of ozone above Madrid (average value of 21.7{+-}1.8 km). The operational ozone AMF calculations for Brewer instruments are limited, in general, to SZA below 80 . Extending the usable SZA range is especially relevant for Brewer instruments located at high mid-latitudes. (orig.)

  3. Defective quiescence entry promotes the fermentation performance of bottom-fermenting brewer's yeast.

    Science.gov (United States)

    Oomuro, Mayu; Kato, Taku; Zhou, Yan; Watanabe, Daisuke; Motoyama, Yasuo; Yamagishi, Hiromi; Akao, Takeshi; Aizawa, Masayuki

    2016-11-01

    One of the key processes in making beer is fermentation. In the fermentation process, brewer's yeast plays an essential role in both the production of ethanol and the flavor profile of beer. Therefore, the mechanism of ethanol fermentation by of brewer's yeast is attracting much attention. The high ethanol productivity of sake yeast has provided a good basis from which to investigate the factors that regulate the fermentation rates of brewer's yeast. Recent studies found that the elevated fermentation rate of sake Saccharomyces cerevisiae species is closely related to a defective transition from vegetative growth to the quiescent (G0) state. In the present study, to clarify the relationship between the fermentation rate of brewer's yeast and entry into G0, we constructed two types of mutant of the bottom-fermenting brewer's yeast Saccharomyces pastorianus Weihenstephan 34/70: a RIM15 gene disruptant that was defective in entry into G0; and a CLN3ΔPEST mutant, in which the G1 cyclin Cln3p accumulated at high levels. Both strains exhibited higher fermentation rates under high-maltose medium or high-gravity wort conditions (20° Plato) as compared with the wild-type strain. Furthermore, G1 arrest and/or G0 entry were defective in both the RIM15 disruptant and the CLN3ΔPEST mutant as compared with the wild-type strain. Taken together, these results indicate that regulation of the G0/G1 transition might govern the fermentation rate of bottom-fermenting brewer's yeast in high-gravity wort. Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  4. Retrieval of aerosol optical depth in the visible range with a Brewer spectrophotometer in Athens

    Science.gov (United States)

    Diémoz, Henri; Eleftheratos, Kostas; Kazadzis, Stelios; Amiridis, Vassilis; Zerefos, Christos S.

    2016-04-01

    A MkIV Brewer spectrophotometer has been operating in Athens since 2004. Direct-sun measurements originally scheduled for nitrogen dioxide retrievals were reprocessed to provide aerosol optical depths (AODs) at a wavelength of about 440 nm. A novel retrieval algorithm was specifically developed and the resulting AODs were compared to those obtained from a collocated Cimel filter radiometer belonging to the Aerosol Robotic Network (AERONET). The series are perfectly correlated, with Pearson's correlation coefficients being as large as 0.996 and with 90 % of AOD deviations between the two instruments being within the World Meteorological Organisation (WMO) traceability limits. In order to reach such a high agreement, several instrumental factors impacting the quality of the Brewer retrievals must be taken into account, including sensitivity to the internal temperature, and the state of the external optics and pointing accuracy must be carefully checked. Furthermore, the long-term radiometric stability of the Brewer was investigated and the performances of in situ Langley extrapolations as a way to track the absolute calibration of the Brewer were assessed. Other sources of error, such as slight shifts of the wavelength scale, are discussed and some recommendations to Brewer operators are drawn. Although MkIV Brewers are rarely employed to retrieve AODs in the visible range, they represent a key source of information about aerosol changes in the past three decades and a potential worldwide network for present and future coordinated AOD measurements. Moreover, a better understanding of the AOD retrieval at visible wavelengths will also contribute in improving similar techniques in the more challenging UV range.

  5. Influence of the ozone profile above Madrid (Spain) on Brewer estimation of ozone air mass factor

    Science.gov (United States)

    Antón, M.; López, M.; Costa, M. J.; Serrano, A.; Bortoli, D.; Bañón, M.; Vilaplana, J. M.; Silva, A. M.

    2009-08-01

    The methodology used by Brewer spectroradiometers to estimate the ozone column is based on differential absorption spectroscopy. This methodology employs the ozone air mass factor (AMF) to derive the total ozone column from the slant path ozone amount. For the calculating the ozone AMF, the Brewer algorithm assumes that the ozone layer is located at a fixed height of 22 km. However, for a real specific site the ozone presents a certain profile, which varies spatially and temporally depending on the latitude, altitude and dynamical conditions of the atmosphere above the site of measurements. In this sense, this work address the reliability of the mentioned assumption and analyses the influence of the ozone profiles measured above Madrid (Spain) in the ozone AMF calculations. The approximated ozone AMF used by the Brewer algorithm is compared with simulations obtained using the libRadtran radiative transfer model code. The results show an excellent agreement between the simulated and the approximated AMF values for solar zenith angle lower than 75°. In addition, the relative differences remain lower than 2% at 85°. These good results are mainly due to the fact that the altitude of the ozone layer assumed constant by the Brewer algorithm for all latitudes notably can be considered representative of the real profile of ozone above Madrid (average value of 21.7±1.8 km). The operational ozone AMF calculations for Brewer instruments are limited, in general, to SZA below 80°. Extending the usable SZA range is especially relevant for Brewer instruments located at high mid-latitudes.

  6. Brewer-Dobson circulation diagnosed from JRA-55

    Science.gov (United States)

    Kobayashi, Chiaki; Iwasaki, Toshiki

    2016-02-01

    We examine the Brewer-Dobson circulation (BDC) in the lower stratosphere diagnosed from the Japanese 55 year Reanalysis (JRA-55) data set and compare it with the BDCs diagnosed from the other reanalyses (i.e., ERA-Interim and JRA-25), and JRA-55-related data sets (i.e., JRA-55C, created by assimilating only in situ observations, and JRA-55AMIP, a simulation by a prediction model). The climatological mean seasonal change of the BDC in JRA-55 is similar to that in ERA-Interim but considerably different from that in JRA-25. Dynamical and thermodynamical consistencies among the variables are greatly improved in the JRA-55 data set. The interannual variations of the annual mean tropical upwellings in JRA-55 are almost coincident with those in JRA-55C. It suggests that the weakly increasing trend of the BDC found in JRA-55 does not have anything to do with changes in the satellite observing system. The climatological mean tropical upwelling diagnosed from JRA-55 is stronger than that from JRA-55AMIP. This difference is presumed to partly link to the model's inability to simulate the quasi-biennial oscillation (QBO). The JRA-55AMIP data set, a simple simulation performed without data assimilation, does not simulate the QBO, whereas the JRA-55 data set represents the QBO with the aid of data assimilation. The climatological mean zonal mean states of JRA-55AMIP considerably deviate from those of JRA-55 in the lower stratosphere. The deviation of JRA-55AMIP is similar to the modulation pattern by the QBO in specific phase regions in which the BDC is rather weak. The simulated QBO might enhance the BDC and reduce the model biases in the tropical lower stratosphere.

  7. Inactivation of MET10 in brewer's yeast specifically increases SO2 formation during beer production.

    Science.gov (United States)

    Hansen, J; Kielland-Brandt, M C

    1996-11-01

    Sulfite is widely used as an antioxidant in food production. In beer brewing, sulfite has the additional role of stabilizing the flavor by forming adducts with aldehydes. Inadequate amounts of sulfite are sometimes produced by brewer's yeasts, so means of controlling the sulfite production are desired. In Saccharomyces yeasts, MET10 encodes a subunit of sulfite reductase. Partial or full elimination of MET10 gene activity in a brewer's yeast resulted in increased sulfite accumulation. Beer produced with such yeasts was quite satisfactory and showed increased flavor stability.

  8. Desempenho de bezerros holandeses alimentados após o desaleitamento com silagem de grãos úmidos ou grãos secos de milho ou sorgo Performance of Holstein calves fed after weaning with high moisture grains silage or dry ground grains of corn or sorghum

    Directory of Open Access Journals (Sweden)

    Gercílio Alves de Almeida Júnior

    2008-01-01

    Full Text Available Avaliou-se o desempenho de bezerros alimentados após desaleitamento e até o abate com silagem de grãos úmidos ou grãos secos de milho ou sorgo para a produção de vitelos de carne rosa. Trinta bezerros holandeses foram distribuídos em delineamento em blocos casualizados, com cinco blocos e seis tratamentos, e alimentados com seis rações concentradas com teores similares de proteína (18,5% PB e energia (3,2 Mcal EM/kg de MS, formuladas com: milho seco moído (MM; silagem de grãos úmidos de milho (SGUM; sorgo seco com tanino moído, (SCTM; silagem de grãos úmidos inteiros de sorgo com tanino (SGUISCT; sorgo seco sem tanino moído (SSTM; e silagem de grãos úmidos inteiros de sorgo sem tanino (SGUISST. Os animais foram recriados em piquetes coletivos até atingirem o peso pré-estabelecido para o abate (170 ± 10 kg PC. Não houve efeito das rações concentradas sobre a altura de cernelha, a idade ao abate, os dias no experimento e os ganhos de peso diários e totais. Os custos por kg de ganho de peso das rações contendo sorgo foram mais baixos que o das rações formuladas com milho. Todos os alimentos avaliados podem ser usados em rações concentradas para bezerros após o aleitamento, pois não comprometem o desempenho e conferem resultados similares.The performance of calves fed after weaning until slaughter with high moisture grains silage or dry ground grains of corn or sorghum was evaluated, for production of pink meat veal. Thirty Holstein calves were allotted to a complete randomized blocks experimental design with five blocks and six concentrate rations with similar contents of protein (18.5% CP and energy (3.2 Mcal ME/kg DM, formulated with dry ground corn (GC, high moisture corn silage (HMCS, dry ground sorghum with tannin (GSWT, high moisture whole sorghum with tannin silage (HMWSWTS, dry ground sorghum without tannin (GSWTT or high moisture whole sorghum without tannin silage (HMWSWTTS. The animals were raised in

  9. Consumo e digestibilidade em ovinos alimentados com dietas contendo grão de milho moído, inteiro ou tratado com uréia, com três níveis de concentrado Food intake and digestibility by sheep fed diets containing ground, whole or urea treated whole corn grains at three concentrate levels

    Directory of Open Access Journals (Sweden)

    Ivonir Taschetto Bolzan

    2007-02-01

    Full Text Available Este estudo foi conduzido usando grão de milho moído, inteiro ou inteiro tratado com uréia, na preparação de dietas contendo 30; 50 ou 70% de concentrado. Foram usados 18 cordeiros castrados, com 34,48kg de peso vivo médio, distribuídos aleatoriamente nos tratamentos, durante três períodos experimentais, para medir-se o consumo e a digestibilidade dos constituintes da matéria seca da dieta. O consumo de matéria seca (CMS, o consumo de proteína bruta (CPB e o consumo de fibra em detergente neutro (CFDN não foram afetados (P>0,05 pelo tratamento do grão de milho. Entretanto, o nível de inclusão de concentrado afetou (PIn this study corn grain was used as whole, ground or urea treated grain to prepare diet containing 30, 50 or 70% concentrate. Eighteen castrated lambs (34.48 average body weight were used during three periods to measure food intake and digestibility. The intake of dry matter (DMI, crude protein (CPI and neutral detergent fiber (NDFI were not affected by treatment of corn grain. Level of concentrate in diet affected (P<0.05 DMI and CPI which reached a maximum at 55.5 and 58.7% concentrate, respectively. The NDFI decreased linearly (P<0.05 with the increasing level of concentrate. Grain treatment did not affected the digestibility of nutrient except for crude protein apparent digestibility which was higher (P<0.05 for diet containing UG. Apparent digestibility of dry and organic matter and total carbohydrates increased linearly with increasing proportion of concentrate in diet. Whole corn grain can be used to prepare concentrate mixtures to be fed to sheep.

  10. An "Alms-Basket" of "Bric-a-Brac": "Brewer's Dictionary of Phrase and Fable".

    Science.gov (United States)

    Bunge, Charles A.

    1999-01-01

    Describes the development and history of "Brewer's Dictionary of Phrase and Fable," a reference source first published in 1870 that includes the etymology of phrases, allusions and words. Discusses reviews that reflected and shaped its status as a standard reference book, describes the current edition, and considers its enduring value. (LRW)

  11. An "Alms-Basket" of "Bric-a-Brac": "Brewer's Dictionary of Phrase and Fable".

    Science.gov (United States)

    Bunge, Charles A.

    1999-01-01

    Describes the development and history of "Brewer's Dictionary of Phrase and Fable," a reference source first published in 1870 that includes the etymology of phrases, allusions and words. Discusses reviews that reflected and shaped its status as a standard reference book, describes the current edition, and considers its enduring value.…

  12. Comparison between Brewer spectrometer, M 124 filter ozonometer and Dobson spectrophotometer

    Science.gov (United States)

    Feister, U.

    1994-01-01

    Concurrent measurements were taken using the Brewer spectrometer no. 30, the filter ozonometer M 124 no. 200 and the Dobson spectrophotometer no. 71 from September 1987 to December 1988 at Potsdam. The performance of the instrument types and the compatibility of ozone data was checked under the conditions of a field measuring station. Total ozone values derived from Dobson AD direct sun measurements were considered as standard. The Dobson instrument had been calibrated at intercomparisons with the World Standard Dobson instrument no. 83 (Boulder) and with the Regional Standard instrument no. 64 (Potsdam), while the Brewer instrument was calibrated several times with the Travelling Standard Brewer no. 17 (Canada). The differences between individual Brewer DS (direct sun) ozone data and Dobson ADDS are within plus or minus 3 percent with half of all differences within plus or minus 1 percent. Less than 0.7 percent of the systematic difference can be due to atmospheric SO2. Due to inadequate regression coefficients Brewer ZB (zenith blue) ozone measurements are by (3...4) percent higher than Dobson ADDS ozone values. M124 DS ozone data are systematically by (1...2) percent higher than Dobson ADDS ozone with 50 percent of the differences within plus or minus 4 percent, but with extreme differences up to plus or minus (20...25) percent. M124 ZB ozone values are by (3...5) percent higher than Dobson ADDS with all the differences within plus or minus 10 percent, i.e. the scatter of differences is smaller for ZB than for M 124 DS measurements, Results for differences in the daily mean ozone values are also addressed. The differences include the uncertainties in the ozone values derived from both types of measurements. They provide an indication of the uncertainty in ozone data and the comparability of ozone values derived from different types of instruments.

  13. Características de carcaça de cordeiros alimentados com grãos e subprodutos da canola = Carcass characteristics in lambs fed with grains and by-products of canola

    Directory of Open Access Journals (Sweden)

    José Carlos Barbosa

    2009-10-01

    Full Text Available O objetivo foi estudar a introdução de 8% de grãos e subprodutos (farelo ou torta da canola em dietas para cordeiros. Para a avaliação das características quantitativas da carcaça, foram utilizadas 24 carcaças de cordeiros, utilizando delineamento inteiramente casualizado. As dietas com média de 15,4% de PB na MS e 80,2% de NDT foram compostas por 40% de feno de capim-Tifton e 60% de concentrado composto por milho em grão, farelo de soja, canola em grão integral, farelo de canola, torta de canola e mistura mineral. A utilização de grãos e subprodutos da canola na dieta de borregos terminados em confinamento não influenciou (p > 0,05 as características quantitativas da carcaça. Em relação aos rendimentos dos cortes, não houve efeito dos tratamentos para nenhuma das variáveis analisadas. Assim, a introdução de 8% de grãos e subprodutos (farelo ou torta da canola possibilitaram bons resultados podendo ser recomendados nas formulações de dietas para cordeiros.The aim of this work was to evaluate the introduction of 8% grains and by-products (meal or cake of canola in the diets of lambs. To evaluate quantitative carcass characteristics, 24 Santa Ines lambs were used in a completely randomized design. Diets with averages of 15.4% of CP in DM and 80.2% of TDN were composed for 40% Tifton hay and 60% concentrate based on corn grain, soybean meal, whole grain canola, canola meal, canola cake and mineral mixture. The use of whole grains and by-products of canola in the diet of lambs finished in feedlot did not influence (p > 0.05 quantitative carcass characteristics. For cut dressing in relation to the CCW, no effect was observed for the analyzed variables among treatments. It was concluded that the introduction of grains and by-products of canola allow for satisfactory results, and could be recommended in the formulations of lamb diets.

  14. Performance of non-conventional yeasts in co-culture with brewers' yeast for steering ethanol and aroma production.

    Science.gov (United States)

    van Rijswijck, Irma M H; Wolkers-Rooijackers, Judith C M; Abee, Tjakko; Smid, Eddy J

    2017-08-18

    Increasing interest in new beer types has stimulated the search for approaches to extend the metabolic variation of brewers' yeast. Therefore, we tested two approaches using non-conventional yeast to create a beer with lower ethanol content and a complex aroma bouquet. First, the mono-culture performance was monitored of 49 wild yeast isolates of Saccharomyces cerevisiae (16 strains), Cyberlindnera fabianii (9 strains) and Pichia kudriavzevii (24 strains). Interestingly, both C. fabianii and P. kudriavzevii isolates produced relatively more esters compared with S. cerevisiae isolates, despite their limited fermentation capacity. Next, one representative strain of each species (Sc131, Cf65 and Pk129) was applied as co-culture with brewers' yeast (ratio 1:1). Co-cultures with Cf65 and Pk129 resulted in a beer with lower alcohol content (3.5, 3.8 compared with 4.2% v/v) and relatively more esters. At higher inoculum ratios of Cf65 over brewers' yeast, growth inhibition of brewers' yeast was observed, most likely caused by competition for oxygen between brewers' yeast and Cf65 resulting in a reduced level of ethanol and altered aroma profiles. With this study, we demonstrate the feasibility of using non-conventional yeast species in co-cultivation with traditional brewers' yeast to tailor aroma profiles as well as the final ethanol content of beer. © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  15. Chromosomal Integration and Expression of Two Bacterial alpha-Acetolactate Decarboxylase Genes in Brewer's Yeast.

    Science.gov (United States)

    Blomqvist, K; Suihko, M L; Knowles, J; Penttilä, M

    1991-10-01

    A bacterial gene encoding alpha-acetolactate decarboxylase, isolated from Klebsiella terrigena or Enterobacter aerogenes, was expressed in brewer's yeast. The genes were expressed under either the yeast phosphoglycerokinase (PGK1) or the alcohol dehydrogenase (ADH1) promoter and were integrated by gene replacement by using cotransformation into the PGK1 or ADH1 locus, respectively, of a brewer's yeast. The expression level of the alpha-acetolactate decarboxylase gene of the PGK1 integrant strains was higher than that of the ADH1 integrants. Under pilot-scale brewing conditions, the alpha-acetolactate decarboxylase activity of the PGK1 integrant strains was sufficient to reduce the formation of diacetyl below the taste threshold value, and no lagering was needed. The brewing properties of the recombinant yeast strains were otherwise unaltered, and the quality (most importantly, the flavor) of the trial beers produced was as good as that of the control beer.

  16. Construction of dextrin and isomaltose-assimilating brewer's yeasts for production of low-carbohydrate beer.

    Science.gov (United States)

    Park, Jin-Yeong; Lee, Ja-Yeon; Choi, Seung-Hyun; Ko, Hyun-Mi; Kim, Il-Chul; Lee, Hwanghee Blaise; Bai, Suk

    2014-08-01

    Most Saccharomyces spp. cannot degrade or ferment dextrin, which is the second most abundant carbohydrate in wort for commercial beer production. Dextrin-degrading brewer's bottom and top yeasts expressing the glucoamylase gene (GAM1) from Debaryomyces occidentalis were developed to produce low-carbohydrate (calorie) beers. GAM1 was constitutively expressed in brewer's yeasts using a rDNA-integration system that contained yeast CUP1 gene coding for copper resistance as a selective marker. The recombinants secreted active glucoamylase, displaying both α-1,4- and α-1,6-debranching activities, that degraded dextrin and isomaltose and consequently grew using them as sole carbon source. One of the recombinant strains expressing GAM1 hydrolyzed 96 % of 2 % (w/v) dextrin and 98 % of 2 % (w/v) isomaltose within 5 days of growth. Growth, substrate assimilation, and enzyme activity of these strains were characterized.

  17. Comparative proteome and transcriptome analysis of lager brewer's yeast in the autolysis process.

    Science.gov (United States)

    Xu, Weina; Wang, Jinjing; Li, Qi

    2014-12-01

    The autolysis of brewer's yeast during beer production has a significant effect on the quality of the final product. In this work, we performed proteome and transcriptome studies on brewer's yeast to examine changes in protein and mRNA levels in the process of autolysis. Protein and RNA samples of the strain Qing2 at two different autolysis stages were obtained for further study. In all, 49 kinds of proteins were considered to be involved in the autolysis response, eight of which were up-regulated and 41 down-regulated. Seven new kinds of proteins emerged during autolysis. Results of comparative analyses showed that important changes had taken place as an adaptive response to autolysis. Functional analysis showed that carbohydrate and energy metabolism, cellular amino acid metabolic processes, cell response to various stresses (such as oxidative stress, salt stress, and osmotic stress), translation and transcription were repressed by the down-regulation of the corresponding proteins, and starvation and DNA damage responses could be induced. The comparison of data on transcriptomes with proteomes demonstrated that most autolysis-response proteins as well as new proteins showed a general correlation between mRNA and protein levels. Thus these proteins were thought to be transcriptionally regulated. These findings provide important information about how brewer's yeast acts to cope with autolysis at molecular levels, which might enhance global understanding of the autolysis process.

  18. Effects of Fermented Distillers' Grains on Performance, Apparent Nutrient Digestibility, Ruminal Fermentation and Serum Biochemical Indices in House-Fed Yaks%发酵酒糟对舍饲牦牛生产性能、养分表观消化率、瘤胃发酵和血清生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    陈光吉; 彭忠利; 宋善丹; 王斌星; 郭春华; 张正帆; 柏雪; 王永; 钟金城

    2015-01-01

    The objective of this study was to determine the effects of fermented distillers' grains supplemented in diets on performance, apparent nutrient digestibility, ruminal fermentation and serum biochemical indices in house-fed yaks. A complete randomized design was adopted in this study, and twenty 3-year-old Maiwa male yaks weighted (208.30±3.31) kg were randomly divided into 2 (A and B) groups with 10 replicates of 1 yak in each group. Yaks in A group were fed corn-soybean diet, and yaks in B group were fed the corn-fermented distillers' grains diet which contain 9. 25% fermented distillers' grains and equal nitrogen content compared with the diet of A group. The experiment lasted for 60 d. The results showed as follows: compared with A group, 1) dry matter intake and daily gain in B group were significantly increased ( P0.05);4) serum to-tal protein and high-density lipoprotein contents in group B were significantly increased (P0.05). Consequently, the diet that contain 9.25% fermented distillers' grains can improve performance and nutrients digestibility in yaks, but does not change the type of rumen fermentation, and has no adverse effects on serum biochemical indices.%本试验旨在研究饲粮中添加发酵酒糟对舍饲牦牛生产性能、养分表观消化率、瘤胃发酵和血清生化指标的影响. 选取20只(208.30±3.31) kg年龄为3周岁的麦洼公牦牛,采用完全随机试验设计,将牦牛随机分为2组( A和B组) ,每组10个重复,每个重复1头牦牛. A组饲喂玉米-豆粕型饲粮,B组饲粮中添加9.25%的发酵酒糟,设计成与A组等氮的玉米-发酵酒糟型饲粮. 试验期60 d. 结果表明:与 A 组相比,1) B 组的干物质采食量和日增重显著提高( P0.05);4) B组显著提高了血清总蛋白和高密度脂蛋白的含量( P0.05). 由此可见,饲粮中添加9.25%的发酵酒糟可提高牦牛的生产性能和对营养物质的消化率,但不改变瘤胃发酵类型,且对血清生化指标无不良影响.

  19. Características de carcaças e dos componentes não-carcaça de bezerros holandeses alimentados após o desaleitamento com silagem de grãos úmidos ou grãos secos de milho ou sorgo Characteristics of carcasses and non carcass components of Holstein calves fed post weaning with high moisture grains silage or dry ground grains of corn or sorghum

    Directory of Open Access Journals (Sweden)

    Gercílio Alves de Almeida Júnior

    2008-01-01

    Full Text Available Avaliaram-se as características de carcaças e dos componentes não-carcaça de bezerros alimentados após desaleitamento até o abate com silagem de grãos úmidos ou grãos secos de milho ou sorgo para a produção de vitelos de carne rosa. Trinta bezerros holandeses foram distribuídos em delineamento em blocos casualizados, com cinco blocos e seis tratamentos, e alimentados com seis rações com teores similares de proteína (18,5% PB e de energia (3,2 Mcal EM/kg de MS, formuladas com: milho seco moído (MM; silagem de grãos úmidos de milho (SGUM; sorgo seco com tanino moído, (SCTM; silagem de grãos úmidos inteiros de sorgo com tanino (SGUISCT; sorgo seco sem tanino moído (SSTM; e silagem de grãos úmidos inteiros de sorgo sem tanino (SGUISST. Os animais foram recriados em piquetes coletivos até atingirem o peso pré-estabelecido para o abate (170 ± 10 kg PV. Não houve efeito da composição das rações concentradas sobre os pesos de carcaça, de cortes e dos componentes não-carcaça nem sobre os rendimentos de carcaça quente e fria, de traseiro e dos outros cortes. Identificou-se efeito das rações concentradas apenas sobre o rendimento de dianteiro, que foi maior nos animais alimentados com SGUISST em comparação àqueles alimentados com MSM e SCTM. Todos os alimentos avaliados podem ser usados em rações concentradas para bezerros após o aleitamento, pois não comprometem as características de carcaça e dos componentes não-carcaça e conferem resultados similares.The characteristics of carcasses and non carcass components of calves fed after weaning until slaughter with high moisture grains silage or dry ground grains of corn or sorghum was evaluated, for production of pink meat veal. Thirty Holstein calves were allotted to a complete randomized blocks experimental design with five blocks and six concentrate rations with similar contents of protein (18.5% CP and energy (3.2 Mcal ME/kg DM, formulated with dry ground

  20. Evaluation of the use of five laboratory determined ozone absorption cross sections in brewer and dobson retrieval algorithms

    Directory of Open Access Journals (Sweden)

    A. Redondas

    2013-09-01

    Full Text Available The primary ground-based instruments used to report total column ozone (TOC are Brewer and Dobson Spectrophotometers, in separate networks. These instruments make measurements of the UV irradiances, and through a well-defined process a TOC value is produced. Inherent in the algorithm is the use of a laboratory determined cross-section data set. We used five ozone cross section data sets: three Bass and Paur, Daumont, Malicet and Brion (DMB and a new Institute of Environmental Physics (IUP, University of Bremen, set. The three Bass and Paur (1985 sets are: quadratic temperature coefficients from IGACO web page (IGQ4, the Brewer network operational calibration set (BOp, and the set used by Bernhard et al. (2005, in the reanalysis of the Dobson absorption coefficient values (B05. The ozone absorption coefficients for Brewer and Dobson are then calculated using the normal Brewer operative method which is essentially the same as used on Dobson. Considering the standard TOC algorithm for the Brewer instruments and comparing to the Brewer standard operational calibration data set, using the slit functions for the individual instruments: we find the UIP data set changes the calculated TOC by −0.5%, the DBM data set changes the calculate TOC by −3.2%, and the IGQ4 data set at −45 °C changes the calculated TOC by +1.3%. Considering the standard algorithm for the Dobson instruments, and comparing to results using the official 1992 ozone absorption coefficients values and the single set of slit functions defined for all Dobson instruments, the calculated TOC changes by +1%, with little variation depending on which data set is used We applied the changes to the European Dobson and Brewer reference instruments during the Izaña 2012 Absolute Calibration Campaign. The application of a common Langley calibration and the IUP cross section the differences between Brewer and Dobson vanish whereas using Bass and Paur and DBM produce differences of 1.5% and 2

  1. Contracts for Grain Biosecurity and Grain Quality

    OpenAIRE

    Abougamos, Hoda; White, Benedict; Sadler,Rohan

    2012-01-01

    The export of grain from Western Australia depends upon a grain supply network that takes grain from farm to port through Cooperative Bulk Handling receival and storage sites. The ability of the network to deliver pest free grain to the port and onto ship depends upon the quality of grain delivered by farmers and the efficacy of phosphine based fumigation in controlling stored grain pests. Phosphine fumigation is critical to the grain supply network because it is the cheapest effective fumiga...

  2. Effect of supplementing grain amaranth diets with amino acids ...

    African Journals Online (AJOL)

    James Bond

    fortification of amaranth diets with casein improved chick performance and the ... animals fed grain amaranth diets has been attributed to anti-nutritional factors in ...... The authors wish to thank the German Academic Exchange Programme ...

  3. Absolute spectral measurements of direct solar ultraviolet irradiance with a Brewer spectrophotometer.

    Science.gov (United States)

    Bais, A F

    1997-07-20

    A methodology for the absolute calibration of spectral measurements of direct solar ultraviolet radiation, performed with a Brewer spectrophotometer is presented. The method uses absolute measurements of global and diffuse solar irradiance obtained practically simultaneously at each wavelength with the direct-Sun component. On the basis of this calibration, direct-Sun spectra, measured over a wide range of solar zenith angles at a high altitude site, were used to determine the extraterrestrial solar spectrum by applying the Langley extrapolation method. Finally this spectrum is compared with a solar spectrum derived from the airborne tunable laser absorption spectrometer 3 Space Shuttle mission, showing an agreement of better than +/-3%.

  4. Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast.

    Science.gov (United States)

    Onnela, M L; Suihko, M L; Penttilä, M; Keränen, S

    1996-08-20

    The bacterial gene, encoding alpha-acetolactate decarboxylase (alpha-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of alpha-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. alpha-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

  5. Grain Spectroscopy

    Science.gov (United States)

    Allamandola, L. J.

    1992-01-01

    Our fundamental knowledge of interstellar grain composition has grown substantially during the past two decades thanks to significant advances in two areas: astronomical infrared spectroscopy and laboratory astrophysics. The opening of the mid-infrared, the spectral range from 4000-400 cm(sup -1) (2.5-25 microns), to spectroscopic study has been critical to this progress because spectroscopy in this region reveals more about a materials molecular composition and structure than any other physical property. Infrared spectra which are diagnostic of interstellar grain composition fall into two categories: absorption spectra of the dense and diffuse interstellar media, and emission spectra from UV-Vis rich dusty regions. The former will be presented in some detail, with the latter only very briefly mentioned. This paper summarized what we have learned from these spectra and presents 'doorway' references into the literature. Detailed reviews of many aspects of interstellar dust are given.

  6. 啤酒糟预处理技术%Research into Pretreatment Methods of Brewer's Spent Grain

    Institute of Scientific and Technical Information of China (English)

    邓启华; 傅力; 王德良; 刘霞; 林智平; 贾凤超

    2010-01-01

    采用蒸汽爆破、超微粉碎、纤维素酶水解预处理技术对啤酒糟进行了预处理,分析了预处理前后啤酒糟纤维形态结构、纤维组分和还原糖的变化,结果表明,蒸汽爆破预处理技术能有效的破坏啤酒糟中的纤维形态结构,降低中性洗涤纤维(NDF)含量,并提高啤酒糟中还原糖的含量;研究并优化了纤维素酶水解啤酒糟的最佳条件是:酶浓度180 U/g,底物浓度为10%,温度为50℃,作用时间为6 h;蒸汽爆破后的啤酒糟经纤维素酶水解后,还原糖净增量达12.77 mg/mL,NDF基本没有变化.

  7. Cultivation of Cordyceps militaris Using Spent Brewer's Grain%啤酒糟栽培蛹虫草

    Institute of Scientific and Technical Information of China (English)

    叶春苗

    2015-01-01

    利用啤酒糟栽培蛹虫草(Cordyceps militaris),通过单因素试验培考察培养基中啤酒糟与大米组成比例、培养基含水量、培养基起始pH、培养温度和光照对蛹虫草子实体产量和质量的影响,在此基础上设计正交试验,通过正交试验选出啤酒糟栽培蛹虫草最佳的栽培条件为培养基啤酒糟与大米比例1:1,含水量65%,瓶底转色后于23℃,光照强度700 lux下培养,在此条件下每瓶产量为3.24 g(每瓶培养料30 g).

  8. Metabolic flux and nodes control analysis of brewer's yeasts under different fermentation temperature during beer brewing.

    Science.gov (United States)

    Yu, Zhimin; Zhao, Haifeng; Zhao, Mouming; Lei, Hongjie; Li, Huiping

    2012-12-01

    The aim of this work was to further investigate the glycolysis performance of lager and ale brewer's yeasts under different fermentation temperature using a combined analysis of metabolic flux, glycolytic enzyme activities, and flux control. The results indicated that the fluxes through glycolytic pathway decreased with the change of the fermentation temperature from 15 °C to 10 °C, which resulted in the prolonged fermentation times. The maximum activities (V (max)) of hexokinase (HK), phosphofructokinase (PFK), and pyruvate kinase (PK) at key nodes of glycolytic pathway decreased with decreasing fermentation temperature, which was estimated to have different control extent (22-84 %) on the glycolytic fluxes in exponential or flocculent phase. Moreover, the decrease of V (max) of PFK or PK displayed the crucial role in down-regulation of flux in flocculent phase. In addition, the metabolic state of ale strain was more sensitive to the variation of temperature than that of lager strain. The results of the metabolic flux and nodes control analysis in brewer's yeasts under different fermentation temperature may provide an alternative approach to regulate glycolytic flux by changing V (max) and improve the production efficiency and beer quality.

  9. Observations and Interpretation of Descent and Mixing in the Northern Hemisphere Brewer Dobson Circulation

    Science.gov (United States)

    Gille, J. C.; Karol, S.; Kinnison, D. E.; Yudin, V.; Nardi, B.

    2012-12-01

    In 1929 Brewer proposed that the motions now known as the Brewer-Dobson (BD) circulation were responsible for the observed high values of ozone at high latitudes, far from their low-latitude region of formation. Here data from the High Resolution Dynamics Limb Sounder (HIRDLS) instrument, with 1 km vertical resolution and 100 km along track spacing, are used examine how this circulation creates that distribution and its seasonal variation through the interactions between the overturning motions of the BD circulation and isentropic mixing in the lower and lowermost stratosphere (350-450K). Isopleths of ozone in equivalent latitude-potential temperature coordinates illustrate the high-latitude descent, controlled by diabatic cooling, from September to the lowest altitudes in January and February. This descent creates large gradients along isentropic surfaces, where mixing above ~ 360K is weak at this time. The strength of mixing is taken to be the effective diffusivity Deff as formulated by Nakamura [1996]. By late winter into spring the BD circulation weakens, as does the transport barrier near 35° associated with the sub-tropical jet. These processes allow strong equator-ward mixing, leading to progressively smaller latitudinal ozone gradients on the isentropes, and the rising and flattening of the ozone isopleths. By the end of the summer the isopleths show only small slopes, preparatory for the next cycle. These results for the ozone dynamics are similar, but not identical, to those obtained with NCAR's Whole Atmosphere Community Climate Model (WACCM) Version 4.

  10. Composição físico-química de carcaças de bezerros holandeses alimentados após o desaleitamento com silagem de grãos úmidos ou grãos secos de milho ou sorgo Physical and chemical carcasses composition of Holstein calves fed after weaning with high moisture grains silage or dry ground grains of corn or sorghum

    Directory of Open Access Journals (Sweden)

    Gercílio Alves de Almeida Júnior

    2008-01-01

    Full Text Available Avaliou-se a composição físico-química de carcaças de bezerros após desaleitamento até o abate com silagem de grãos úmidos ou grãos secos de milho ou sorgo para a produção de vitelos de carne rosa. Trinta bezerros holandeses foram distribuídos em delineamento em blocos casualizados, com cinco blocos e seis tratamentos, e alimentados com seis rações com teores similares de proteína (18,5% PB e de energia (3,2 Mcal EM/kg de MS, formuladas com: milho seco moído (MM; silagem de grãos úmidos de milho (SGUM; sorgo seco com tanino moído (SCTM; silagem de grãos úmidos inteiros de sorgo com tanino (SGUISCT; sorgo seco sem tanino moído; e silagem de grãos úmidos inteiros de sorgo sem tanino (SGUISST. Após o resfriamento, foram tomados nas meias-carcaças esquerdas os cortes da seção H&H para estimativa da composição tecidual da carcaça e os cortes no Longissimus dorsi à altura da 12ª e 13ª costelas para determinação da área de olho-de-lombo (AOL, da espessura de gordura subcutânea (EGS, da força de cisalhamento (FC e da composição química. Não houve efeito da composição das rações concentradas sobre a composição física e a relação entre tecidos na seção H&H, bem como para AOL, EGS e FC. A ração concentrada SGUM resultou em maiores teores de EE no Longissimus dorsi, mas não diferiu das rações SCTM e SSTM. A ração concentrada SGUM gerou maior deposição de gordura muscular em comparação à MM, no entanto, as rações SGUISCT e SGUISST não aumentaram essa deposição em comparação ao fornecimento dos grãos secos moídos. Todos os alimentos avaliados podem ser usados em rações concentradas para bezerros após o aleitamento, pois não comprometem a composições física e química da carca��a e conferem resultados similares.The physical and chemical carcasses composition of calves fed after weaning until slaughter with high moisture grains silage or dry ground grains of corn or sorghum

  11. Validation of OMI-TOMS and OMI-DOAS total ozone column using five Brewer spectroradiometers at the Iberian peninsula

    Science.gov (United States)

    Antón, M.; López, M.; Vilaplana, J. M.; Kroon, M.; McPeters, R.; Bañón, M.; Serrano, A.

    2009-07-01

    This article focuses on the comparison of the total ozone column data from the Ozone Monitoring Instrument (OMI) flying aboard the NASA EOS-Aura satellite platform with ground-based measurement recorded by Brewer spectroradiometers located at five Spanish remote sensing ground stations between January 2005 and December 2007. The satellite data are derived from two algorithms: OMI Total Ozone Mapping Spectrometer (OMI-TOMS) and OMI Differential Optical Absorption Spectroscopy (OMI-DOAS). The largest relative differences between these OMI total ozone column estimates reach 5% with a significant seasonal dependence. The agreement between OMI ozone data and Brewer measurements is excellent. Total ozone columns from OMI-TOMS are on average a mere 2.0% lower than Brewer data. For OMI-DOAS data the bias is a mere 1.4%. However, the relative difference between OMI-TOMS and Brewer measurements shows a notably lower seasonal dependence and variability than the differences between OMI-DOAS and ground-based data. For both OMI ozone data products these relative differences show significant dependence on the satellite ground pixel solar zenith angle for cloud-free cases as well as for cloudy conditions. However, the OMI ozone data products are shown to reveal opposite behavior with respect to the two antagonistic sky conditions. No significant dependency of the ground-based to satellite-based differences with respect to the satellite cross-track position is seen for either OMI retrieval algorithm.

  12. An improved algorithm for the determination of aerosol optical depth in the ultraviolet spectral range from Brewer spectrophotometer observations

    Science.gov (United States)

    Sellitto, P.; di Sarra, A.; Siani, A. M.

    2006-10-01

    Methods to derive aerosol optical depth in the UV spectral range from ground-based remote-sensing stations equipped with Brewer spectrophotometers have been recently developed. In this study a modified Langley plot method has been implemented to retrieve aerosol optical depth from direct sun Brewer measurements. The method uses measurements over an extended range of atmospheric airmasses obtained with two different neutral density filters, and accounts for short-term variations of total ozone, derived from the same direct sun observations. The improved algorithm has been applied to data collected with a Brewer mark IV, operational in Rome, Italy, and with a Brewer mark III, operational in Lampedusa, Italy, in the Mediterranean. The efficiency of the improved algorithm has been tested comparing the number of determinations of the extraterrestrial constant against those obtained with a standard Langley plot procedure. The improved method produces a larger number of reliable Langley plots, allowing for a better statistical characterization of the extraterrestrial constant and a better study of its temporal variability. The values of aerosol optical depth calculated in Rome and Lampedusa compare well with simultaneous determinations in the 416-440 nm interval derived from MFRSR and CIMEL measurements.

  13. 27 CFR 25.160 - Tax adjustment for brewers who produce more than 2,000,000 barrels of beer.

    Science.gov (United States)

    2010-04-01

    ... who produce more than 2,000,000 barrels of beer. 25.160 Section 25.160 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Tax on Beer Determination of Tax § 25.160 Tax adjustment for brewers who produce more than 2,000,000 barrels of beer....

  14. Arctic total ozone trend and variability during 2004 - 2012 based on Brewer revised data, Ozonesonde and satellite measurements

    Science.gov (United States)

    Moeini, Omid; Vaziri, Zahra; McElroy, Tom; Tarasick, David; Savastiouk, Vladimir; Barton, David

    2015-04-01

    It is now known that Single-Monochromator Brewer Spectrophotometer ozone measurements suffer from non-linearity due to the presence of instrumental stray light caused by scattering from the optics within the instrument. Stray light is unwanted radiation from different wavelengths that arrives at the detector during the measurements. Since the gradient of ozone absorption is large in the ultraviolet spectral region, the stray light contribution becomes significant between 300 and 325 nm where the Brewer measures, especially when the amount of ozone in the light path becomes more than 1000 Dobson Units (D.U.). Stray light results in an underestimated ozone column at larger air masses. As the light path (air mass) increases, stray-light effects in the measurements also increase. An ozone column of 600 D.U. with at an air mass factor of 3 (1800 D.U.) can measure as much as 8% lower than the ozone actual amount. These are conditions commonly seen during the Arctic spring. A new method to account for stray light effects is being developed for the Brewer ozone measurements. This method is based on a mathematical model of the instrument response and a non-linear retrieval which calculates the best values for the model parameters. The parameterization used is validated by an instrument physical model simulation. Using the mathematical model in reverse provides correct ozone values. This paper presents the method and the results of a trend analysis based of the re-evaluated data of three Brewers which are located in the Arctic (Alert Lat. 82.44, Lon. -62.55, Eureka Lat. 79.96, Lon. -86.45 and Resolute Lat. 74.69, and Lon. -95.01) from 2004 to 2012. Gaps in the Brewer data are filled with ozonesonde reanalysis data obtained from WOUDC (World Ozone and Ultraviolet radiation Data Centre) and the results will be compared with MLS (Microwave Limb Sounder) satellite data.

  15. Validation of OMI total ozone retrievals from the SAO ozone profile algorithm and three operational algorithms with Brewer measurements

    Directory of Open Access Journals (Sweden)

    J. Bak

    2014-02-01

    Full Text Available The accuracy of total ozone computed from the Smithsonian Astrophysical Observatory (SAO optimal estimation (OE ozone profile algorithm (SOE applied to the Ozone Monitoring Instrument (OMI is assessed through comparisons with ground-based Brewer spectrometer measurements from 2005 to 2008. We also make comparisons with the three OMI operational ozone products, derived from the NASA Total Ozone Mapping Spectrometer (TOMS, KNMI Differential Optical Absorption Spectroscopy (DOAS, and KNMI OE (KOE algorithms. Excellent agreement is observed between SAO and Brewer, with a mean difference of less than ±1% at most individual stations. The KNMI OE algorithm systematically overestimates Brewer total ozone by 2% at low/mid latitudes and 5% at high latitudes while the TOMS and DOAS algorithms underestimate it by ~1.65% on average. Standard deviations of ~1.8% are found for both SOE and TOMS, but DOAS and KOE have scatters of 2.2% and 2.6%, respectively. The stability of the SOE algorithm is found to have insignificant dependence on viewing geometry, cloud parameters, total ozone column. In comparison, the KOE differences to Brewer values are significantly correlated with solar and viewing zenith angles, with a significant deviation depending on cloud parameters and total ozone amount. The TOMS algorithm exhibits similar stability to SOE with respect to viewing geometry and total column ozone, but stronger cloud parameter dependence. The dependence of DOAS on the algorithmic variables is marginal compared to KOE, but distinct compared to the SOE and TOMS algorithms. Comparisons of All four OMI products with Brewer show no apparent long-term drift but a seasonally affected feature, especially for KOE and TOMS. The substantial differences in the KOE vs. SOE algorithm performance cannot be sufficiently explained by the use of soft calibration (in SOE and the use of different a priori error covariance matrix, but other algorithm details cause larger fitting

  16. The efficacy of a new 6-phytase obtained from Buttiauxella spp. expressed in Trichoderma reesei on digestibility of amino acids, energy, and nutrients in pigs fed a diet based on corn, soybean meal, wheat middlings, and corn distillers' dried grains with solubles.

    Science.gov (United States)

    Adedokun, S A; Owusu-Asiedu, A; Ragland, D; Plumstead, P; Adeola, O

    2015-01-01

    Sixteen cannulated pigs were used to evaluate the effect of a new 6-phytase derived from Buttiauxella spp. and expressed in Trichoderma reesei on apparent ileal digestibility (AID) of AA and apparent total tract digestibility (ATTD) of DM, N, Ca, P, Na, Mg, K, Cl, and energy. Pigs were fed 4 diets for 2 periods in a crossover design. Within each period, there were 4 blocks of 4 pigs per block with each diet represented within each block. The average initial BW in periods 1 and 2 were 22 and 30 kg, respectively. Each period lasted 9 d with fecal collection on d 5 and 6 and a 12-h ileal digesta collection on d 7, 8, and 9. Pigs received a daily feed allowance of approximately 4.5% of their BW. The experimental diets were based on corn, soybean meal, wheat middlings, and corn distillers dried grain with solubles. Phytase was added at 0; 500; 1,000; or 2,000 phytase units/kg of diet to a basal diet that contained 205, 15, 5.4, and 10 g of CP, Lys, total P (1.6 g of nonphytate P), and Ca/kg diet, respectively. The addition of phytase improved (P phytase supplementation linearly and quadratically increased (P Phytase supplementation of the basal diet improved (P Phytase supplementation increased (P phytase supplementation of the basal diet increased (P phytase supplementation to the basal diet showed a tendency (P phytase supplementation. Increasing the level of phytase supplementation resulted in linear increases (P phytase expressed in Trichoderma reesei enhanced ileal digestibility of N and several AA in growing pigs in a dose-dependent manner.

  17. Influence of quantities of brewer yeast on the performance of Anastrepha obliqua wild females (Diptera, Tephritidae

    Directory of Open Access Journals (Sweden)

    Cresoni-Pereira Carla

    2001-01-01

    Full Text Available Using artificial solid diets, experiments were performed with Anastrepha obliqua (Macquart, 1835 wild females in order to verify the influence of different quantities of brewer yeast on the performance and compensation behavior to unbalanced diets ingestion. The observed parameters were egg production, ingestion, diet efficiency and survival in the reproductive phase. Results indicated that there was no compensatory ingestion to different quantities of yeast and that the diet with 12.5g of yeast provided the best performance. The absence of compensatory ingestion is discussed based on the yeast phagostimulation and on the costs involved in solid diets ingestion. The relation between the analyzed parameters and the protein quantities in the diet were discussed.

  18. Influence of quantities of brewer yeast on the performance of Anastrepha obliqua wild females (Diptera, Tephritidae)

    Energy Technology Data Exchange (ETDEWEB)

    Cresoni-Pereira, Carla; Zucoloto, Fernando Sergio [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Filosofia, Ciencias e Letras. Dept. de Biologia

    2001-11-15

    Using artificial solid diets, experiments were performed with Anastrepha obliqua (Macquart, 1835) wild females in order to verify the influence of different quantities of brewer yeast on the performance and compensation behavior to unbalanced diets ingestion. The observed parameters were egg production, ingestion, diet efficiency and survival in the reproductive phase. Results indicated that there was no compensatory ingestion to different quantities of yeast and that the diet with 12.5g of yeast provided the best performance. The absence of compensatory ingestion is discussed based on the yeast phagostimulation and on the costs involved in solid diets ingestion. The relation between the analyzed parameters and the protein quantities in the diet were discussed. (author)

  19. Preliminary crystallographic data for the thiamin diphosphate-dependent enzyme pyruvate decarboxylase from brewers' yeast.

    Science.gov (United States)

    Dyda, F; Furey, W; Swaminathan, S; Sax, M; Farrenkopf, B; Jordan, F

    1990-10-15

    Single crystals of the thiamin diphosphate (the vitamin B1 coenzyme)-dependent enzyme pyruvate decarboxylase (EC 4.1.1.1) from brewers' yeast have been grown using polyethylene glycol as a precipitating agent. Crystals of the homotetrameric version alpha 4 of the holoenzyme are triclinic, space group P1, with cell constants a = 81.0, b = 82.4, c = 116.6 A, alpha = 69.5 beta = 72.6, gamma = 62.4 degrees. The crystals are reasonably stable in a rotating anode x-ray beam and diffract to at least 2.5 A resolution. The Vm value of 2.55 A/dalton is consistent with a unit cell containing four subunits with mass of approximately 60 kDa each. Rotation function results with native data indicate strong non-crystallographic 222 symmetry relating the four identical subunits, thus density averaging methods are likely to play a role in the structure determination.

  20. Climate change reduces warming potential of nitrous oxide by an enhanced Brewer-Dobson circulation

    Science.gov (United States)

    Kracher, Daniela; Reick, Christian H.; Manzini, Elisa; Schultz, Martin G.; Stein, Olaf

    2016-06-01

    The Brewer-Dobson circulation (BDC), which is an important driver of the stratosphere-troposphere exchange, is expected to accelerate with climate change. One particular consequence of this acceleration is the enhanced transport of nitrous oxide (N2O) from its sources at the Earth's surface toward its main sink region in the stratosphere, thus inducing a reduction in its lifetime. N2O is a potent greenhouse gas and the most relevant currently emitted ozone-depleting substance. Here we examine the implications of a reduced N2O lifetime in the context of climate change. We find a decrease in its global warming potential (GWP) and, due to a decline in the atmospheric N2O burden, also a reduction in its total radiative forcing. From the idealized transient global warming simulation we can identify linear regressions for N2O sink, lifetime, and GWP with temperature rise. Our findings are thus not restricted to a particular scenario.

  1. Dynamical forcing of sub-seasonal variability in the tropical Brewer-Dobson circulation

    Science.gov (United States)

    Abalos, Marta; Randel, William; Serrano, Encarna

    2014-05-01

    Upwelling across the tropical tropopause exhibits strong sub-seasonal variability superimposed on the well-known annual cycle, and these variations directly affect temperature and tracers in the tropical lower stratosphere. The dynamical forcing of tropical upwelling on sub-seasonal timescales is investigated using the ERA-Interim reanalysis for 1979-2011. Momentum balance diagnostics reveal that transience is linked to the effects of extratropical wave forcing, with centers of action in the extratropical winter stratosphere and in the subtropical upper troposphere of both hemispheres. From a diagnostic point of view, the zonal-mean wind transient response is important for communicating the remote wave forcing to the tropical stratosphere. Dynamical patterns reflect distinctive forcing of the shallow versus deep branches of the Brewer-Dobson circulation.

  2. The examination of parameters for lactic acid fermentation and nutritive value of fermented juice of beetroot, carrot and brewer’s yeast autolysate

    Directory of Open Access Journals (Sweden)

    MILAN MAKSIMOVIC

    2004-09-01

    Full Text Available The conditions for lactic acid fermentation based on a mixture of beetoot juice (Beta vulgaris L. and carrot juice (Daucus carota L. and different content of brewer’s yeast autolysate with Lactobacillus plantarum A112 and with Lactobacillus acidophilus NCDO 1748 has been studied. Both cultures showed good biochemical activity in these mixtures. The production of lactic acid has been stimulated using a higher content of brewer’s yeast autolysate. In these mixtures, L. plantarum A112 showed better growth and lactic acid production than L. acidophilus NCDO 1748. From the data obtained through chemical analyses of the fermented products, it can be seen that the mixture of beetroot and carrot juice and brewer’s yeast autolysate is richer in minerals (Ca, P, Fe and b-carotene than fermented beetroot juice with the same content of brewer’s yeast autolysate.

  3. Isolation and Characterization of Brewer's Yeast Variants with Improved Fermentation Performance under High-Gravity Conditions▿

    Science.gov (United States)

    Blieck, Lies; Toye, Geert; Dumortier, Françoise; Verstrepen, Kevin J.; Delvaux, Freddy R.; Thevelein, Johan M.; Van Dijck, Patrick

    2007-01-01

    To save energy, space, and time, today's breweries make use of high-gravity brewing in which concentrated medium (wort) is fermented, resulting in a product with higher ethanol content. After fermentation, the product is diluted to obtain beer with the desired alcohol content. While economically desirable, the use of wort with an even higher sugar concentration is limited by the inability of brewer's yeast (Saccharomyces pastorianus) to efficiently ferment such concentrated medium. Here, we describe a successful strategy to obtain yeast variants with significantly improved fermentation capacity under high-gravity conditions. We isolated better-performing variants of the industrial lager strain CMBS33 by subjecting a pool of UV-induced variants to consecutive rounds of fermentation in very-high-gravity wort (>22° Plato). Two variants (GT336 and GT344) showing faster fermentation rates and/or more-complete attenuation as well as improved viability under high ethanol conditions were identified. The variants displayed the same advantages in a pilot-scale stirred fermenter under high-gravity conditions at 11°C. Microarray analysis identified several genes whose altered expression may be responsible for the superior performance of the variants. The role of some of these candidate genes was confirmed by genetic transformation. Our study shows that proper selection conditions allow the isolation of variants of commercial brewer's yeast with superior fermentation characteristics. Moreover, it is the first study to identify genes that affect fermentation performance under high-gravity conditions. The results are of interest to the beer and bioethanol industries, where the use of more-concentrated medium is economically advantageous. PMID:17158628

  4. Isolation and characterization of brewer's yeast variants with improved fermentation performance under high-gravity conditions.

    Science.gov (United States)

    Blieck, Lies; Toye, Geert; Dumortier, Françoise; Verstrepen, Kevin J; Delvaux, Freddy R; Thevelein, Johan M; Van Dijck, Patrick

    2007-02-01

    To save energy, space, and time, today's breweries make use of high-gravity brewing in which concentrated medium (wort) is fermented, resulting in a product with higher ethanol content. After fermentation, the product is diluted to obtain beer with the desired alcohol content. While economically desirable, the use of wort with an even higher sugar concentration is limited by the inability of brewer's yeast (Saccharomyces pastorianus) to efficiently ferment such concentrated medium. Here, we describe a successful strategy to obtain yeast variants with significantly improved fermentation capacity under high-gravity conditions. We isolated better-performing variants of the industrial lager strain CMBS33 by subjecting a pool of UV-induced variants to consecutive rounds of fermentation in very-high-gravity wort (>22 degrees Plato). Two variants (GT336 and GT344) showing faster fermentation rates and/or more-complete attenuation as well as improved viability under high ethanol conditions were identified. The variants displayed the same advantages in a pilot-scale stirred fermenter under high-gravity conditions at 11 degrees C. Microarray analysis identified several genes whose altered expression may be responsible for the superior performance of the variants. The role of some of these candidate genes was confirmed by genetic transformation. Our study shows that proper selection conditions allow the isolation of variants of commercial brewer's yeast with superior fermentation characteristics. Moreover, it is the first study to identify genes that affect fermentation performance under high-gravity conditions. The results are of interest to the beer and bioethanol industries, where the use of more-concentrated medium is economically advantageous.

  5. Influência do grão de sorgo como fonte de amido em ovinos alimentados com feno: parâmetros plasmáticos Influence of sorghum grain as a source of starch in sheep fed hay: plasma parameters

    Directory of Open Access Journals (Sweden)

    Jorge López

    2000-08-01

    Full Text Available O objetivo deste trabalho experimental foi verificar a influência de diferentes níveis de grão de sorgo, como fonte de amido, nos parâmetros plasmáticos em ovinos alimentados com feno de capim-elefante (Pennisetum purpureum Schum. O sorgo foi utilizado em quatro níveis na dieta: 0, 15, 30 e 45%. Foram usados 12 ovinos machos castrados distribuídos em quatro tratamentos com três repetições. Foram coletadas seis amostras de sangue por animal logo antes da refeição da manhã (hora zero e 1, 2, 3, 4, 6 e 8 horas após. O delineamento experimental foi o completamente casualizado. A 1ª hora após a refeição apresentou a maior concentração plasmática de uréia (53,3 mg/100 mL e foi superior à 6ª e 8ª hora (49,5 e 49,3 mg/100 mL. A maior concentração de uréia no plasma coincidiu com a maior concentração de amônia no líquido ruminal. O tratamento com 30% de sorgo na dieta apresentou concentração plasmática de glicose de 81,0 mg/100 mL e foi superior ao tratamento testemunha (60,4 mg/100 mL. A concentração de insulina variou entre tratamentos para cada hora de coleta após a refeição, de acordo com os níveis de sorgo na dieta. O tratamento com 45% de sorgo apresentou a maior concentração do hormônio no plasma. Observou-se para todos os tratamentos um pico de produção do hormônio na 4ª hora após a refeição.The objective of this experimental work was to evaluate the influence of different levels of sorghum grain, as a source of starch, on the plasma parameters of sheep fed elephant grass hay (Pennisetum purpureum Schum. Sorghum grain was included in the diet at four levels: 0, 15, 30 e 45%. Twelve castrated male sheep were assigned to four treatments with three replicates. Six samples of blood per animal were collected just before the morning feeding (zero hour and after 1, 2, 3, 4, 6 and 8 hours. A completely randomized design was used. The first hour after feeding showed the highest plasma concentration of

  6. Effects of wort gravity and nitrogen level on fermentation performance of brewer's yeast and the formation of flavor volatiles.

    Science.gov (United States)

    Lei, Hongjie; Zhao, Haifeng; Yu, Zhimin; Zhao, Mouming

    2012-03-01

    Normal gravity wort and high gravity wort with different nitrogen levels were used to examine their effects on the fermentation performance of brewer's yeast and the formation of flavor volatiles. Results showed that both the wort gravity and nitrogen level had significant impacts on the growth rate, viability, flocculation, and gene expression of brewer's yeast and the levels of flavor volatiles. The sugar (glucose, maltose, and maltotriose) consumption rates and net cell growth decreased when high gravity worts were used, while these increased with increasing nitrogen level. Moreover, high gravity resulted in lower expression levels of ATF1, BAP2, BAT1, HSP12, and TDH, whereas the higher nitrogen level caused higher expression levels for these genes. Furthermore, the lower nitrogen level resulted in increases in the levels of higher alcohols and esters at high wort gravity. All these results demonstrated that yeast physiology and flavor balance during beer brewing were significantly affected by the wort gravity and nitrogen level.

  7. Comparison of GOME-2/MetOp total ozone data with Brewer spectroradiometer data over the Iberian Peninsula

    Energy Technology Data Exchange (ETDEWEB)

    Anton, M.; Serrano, A. [Universidad de Extremadura, Badajoz (Spain). Dept. de Fisica; Loyola, D.; Zimmer, W. [German Aerospace Center (DLR), Wessling (DE). Remote Sensing Technology Inst. (IMF); Lopez, M.; Banon, M. [Agencia Estatal de Meteorologia (AEMet), Madrid (Spain); Vilaplana, J.M. [Instituto Nacional de Tecnica Aeroespacial (INTA), Huelva (Spain). Estacion de Sondeos Atmosferico ' ' El Arenosillo' '

    2009-07-01

    The main objective of this article is to compare the total ozone data from the new Global Ozone Monitoring Experiment instrument (GOME-2/MetOp) with reliable ground-based measurement recorded by five Brewer spectroradiometers in the Iberian Peninsula. In addition, a similar comparison for the predecessor instrument GOME/ERS-2 is described. The period of study is a whole year from May 2007 to April 2008. The results show that GOME-2/MetOp ozone data already has a very good quality, total ozone columns are on average 3.05% lower than Brewer measurements. This underestimation is higher than that obtained for GOME/ERS-2 (1.46%). However, the relative differences between GOME-2/MetOp and Brewer measurements show significantly lower variability than the differences between GOME/ERS-2 and Brewer data. Dependencies of these relative differences with respect to the satellite solar zenith angle (SZA), the satellite scan angle, the satellite cloud cover fraction (CF), and the ground-based total ozone measurements are analyzed. For both GOME instruments, differences show no significant dependence on SZA. However, GOME-2/MetOp data show a significant dependence on the satellite scan angle (+1.5%). In addition, GOME/ERS-2 differences present a clear dependence with respect to the CF and ground-based total ozone; such differences are minimized for GOME-2/MetOp. The comparison between the daily total ozone values provided by both GOME instruments shows that GOME-2/MetOp ozone data are on average 1.46% lower than GOME/ERS-2 data without any seasonal dependence. Finally, deviations of a priori climatological ozone profile used by the satellite retrieval algorithm from the true ozone profile are analyzed. Although excellent agreement between a priori climatological and measured partial ozone values is found for the middle and high stratosphere, relative differences greater than 15% are common for the troposphere and lower stratosphere. (orig.)

  8. Comparison of GOME-2/MetOp total ozone data with Brewer spectroradiometer data over the Iberian Peninsula

    Science.gov (United States)

    Antón, M.; Loyola, D.; López, M.; Vilaplana, J. M.; Bañón, M.; Zimmer, W.; Serrano, A.

    2009-04-01

    The main objective of this article is to compare the total ozone data from the new Global Ozone Monitoring Experiment instrument (GOME-2/MetOp) with reliable ground-based measurement recorded by five Brewer spectroradiometers in the Iberian Peninsula. In addition, a similar comparison for the predecessor instrument GOME/ERS-2 is described. The period of study is a whole year from May 2007 to April 2008. The results show that GOME-2/MetOp ozone data already has a very good quality, total ozone columns are on average 3.05% lower than Brewer measurements. This underestimation is higher than that obtained for GOME/ERS-2 (1.46%). However, the relative differences between GOME-2/MetOp and Brewer measurements show significantly lower variability than the differences between GOME/ERS-2 and Brewer data. Dependencies of these relative differences with respect to the satellite solar zenith angle (SZA), the satellite scan angle, the satellite cloud cover fraction (CF), and the ground-based total ozone measurements are analyzed. For both GOME instruments, differences show no significant dependence on SZA. However, GOME-2/MetOp data show a significant dependence on the satellite scan angle (+1.5%). In addition, GOME/ERS-2 differences present a clear dependence with respect to the CF and ground-based total ozone; such differences are minimized for GOME-2/MetOp. The comparison between the daily total ozone values provided by both GOME instruments shows that GOME-2/MetOp ozone data are on average 1.46% lower than GOME/ERS-2 data without any seasonal dependence. Finally, deviations of a priori climatological ozone profile used by the satellite retrieval algorithm from the true ozone profile are analyzed. Although excellent agreement between a priori climatological and measured partial ozone values is found for the middle and high stratosphere, relative differences greater than 15% are common for the troposphere and lower stratosphere.

  9. VizieR Online Data Catalog: C/O and Mg/Si for solar neighborhood's stars (Brewer+, 2016)

    Science.gov (United States)

    Brewer, J. M.; Fischer, D. A.

    2017-01-01

    The catalog of Brewer+ (2016, J/ApJS/225/32) has abundances of 15 elements, including C, O, Mg, and Si, for more than 1600 F, G, and K stars. The stars were all observed using the HIRES instrument on the Keck telescope with the same instrumental setup. Most of the stars were observed as part of the California Planet Search (CPS) program and have a typical S/N>>100. (1 data file).

  10. Panax Ginseng in combination with brewers' yeast (Gerivet) as a stimulant for geriatric dogs: a controlled-randomized blinded study.

    Science.gov (United States)

    Hielm-Björkman, A; Reunanen, V; Meri, P; Tulamo, R-M

    2007-08-01

    The study was performed on two groups of dogs, one (n = 41) given Ginseng (Panax Ginseng) together with brewers' yeast (Saccharomyces cerevisae) and the other (n = 39) given only brewers' yeast (control group, but not a true placebo), for 8 weeks. Using a questionnaire and three visual analogue scales, the blinded owners evaluated the dogs before the trial, weekly for the 8 weeks of the trial and twice, at 12th and 16th weeks, after the trial (follow-up). At 8th, 12th and 16th weeks the owners also answered questions concerning what treatment their dogs likely had been getting, willingness to continue medication and the dogs' general status. The changes from baseline to the end of the treatment period in the variable scores were calculated for each dog and used in statistics. Panax Ginseng plus yeast significantly improved all evaluated variables within the group. Four of the seven primary (mentally) outcome measures were significant when comparing the changes in the Ginseng group with the control group, and six of the seven were significant when compared to an external group. As the secondary (physical) outcome measures were significantly better in both the Ginseng and the control group compared to the external group, it indicates that brewers' yeast is the ingredient that has impact on physical performance. No significant changes in blood- or urine analyses and no side effects were seen.

  11. Effects of nitrogen composition on fermentation performance of brewer's yeast and the absorption of peptides with different molecular weights.

    Science.gov (United States)

    Mo, Fen; Zhao, Haifeng; Lei, Hongjie; Zhao, Mouming

    2013-11-01

    Four kinds of worts with different nitrogen compositions were used to examine their effects on fermentation performance of brewer's yeast. The absorption pattern of peptides with different molecular weights (Mw) in yeast cells during wort fermentation was also investigated. Results showed that both the nitrogen composition and level had significant impacts on the yeast biomass accumulation, ethanol production, and free amino nitrogen and sugars consumption rates. Worts supplemented with wheat gluten hydrolysates increased 11.5% of the biomass, 5.9% of fermentability, and 0.6% of ethanol content and decreased 25.6% of residual sugar content during wort fermentation. Moreover, yeast cells assimilated peptides with various Mw differently during fermentation. Peptides with Mw below 1 kDa decreased quickly, and the rate of assimilation was more than 50% at the end of fermentation, while those with Mw above 10 kDa almost could not be assimilated by yeast. All these results further indicated that the level and composition of wort nitrogen had significant impacts on the growth and fermentation performances of brewer's yeast, and peptides with Mw below 1 kDa were one of preferred nitrogen sources for brewer's yeast.

  12. Physiological characterization of brewer's yeast in high-gravity beer fermentations with glucose or maltose syrups as adjuncts.

    Science.gov (United States)

    Piddocke, Maya P; Kreisz, Stefan; Heldt-Hansen, Hans Peter; Nielsen, Kristian Fog; Olsson, Lisbeth

    2009-09-01

    High-gravity brewing, which can decrease production costs by increasing brewery yields, has become an attractive alternative to traditional brewing methods. However, as higher sugar concentration is required, the yeast is exposed to various stresses during fermentation. We evaluated the influence of high-gravity brewing on the fermentation performance of the brewer's yeast under model brewing conditions. The lager brewer's strain Weihenstephan 34/70 strain was characterized at three different gravities by adding either glucose or maltose syrups to the basic wort. We observed that increased gravity resulted in a lower specific growth rate, a longer lag phase before initiation of ethanol production, incomplete sugar utilization, and an increase in the concentrations of ethyl acetate and isoamyl acetate in the final beer. Increasing the gravity by adding maltose syrup as opposed to glucose syrup resulted in more balanced fermentation performance in terms of higher cell numbers, respectively, higher wort fermentability and a more favorable flavor profile of the final beer. Our study underlines the effects of the various stress factors on brewer's yeast metabolism and the influence of the type of sugar syrups on the fermentation performance and the flavor profile of the final beer.

  13. Effects of chromium brewer's yeast supplementation on body mass, blood carbohydrates, and lipids and minerals in type 2 diabetic patients.

    Science.gov (United States)

    Król, Ewelina; Krejpcio, Zbigniew; Byks, Hanna; Bogdański, Paweł; Pupek-Musialik, Danuta

    2011-11-01

    Chromium(III) is considered as an essential element for carbohydrate and lipid metabolism. The aim of this clinical study was to evaluate the efficacy of Cr brewer's yeast supplementation on body mass, carbohydrate, lipids and mineral indices in type 2 diabetic patients. Twenty adult type 2 diabetic subjects (11 males and 9 females aged 37-63) were supplemented with Cr brewer's yeast in dosages of 500 μg Cr/person/day or placebo for 8 weeks in a double-blind, placebo-controlled crossover design. It was found that supplemental Cr did not affect body mass, blood lipid profile, resistin levels, and the serum and hair Zn, Fe, and Cu levels, but increased serum Cr (by 116%) and hair Cr (by 20.6%) concentrations and improved some blood carbohydrate indices (significant increase in the β cell function index by 18.8%) in type 2 diabetic patients. In conclusion, Cr brewer's yeast has a weak hypoglycemic potential, but does not affect body mass, blood biochemical profile, and microelement levels in type 2 diabetic subjects.

  14. Phytoceramide and sphingoid bases derived from brewer's yeast Saccharomyces pastorianus activate peroxisome proliferator-activated receptors

    Directory of Open Access Journals (Sweden)

    Mitsutake Susumu

    2011-08-01

    Full Text Available Abstract Background Peroxisome proliferator-activated receptors (PPARs are ligand-activated transcription factors that regulate lipid and glucose metabolism. PPARα is highly expressed in the liver and controls genes involved in lipid catabolism. We previously reported that synthetic sphingolipid analogs, part of which contains shorter-length fatty acid chains than natural sphingolipids, stimulated the transcriptional activities of PPARs. Sphingosine and dihydrosphingosine (DHS are abundant sphingoid bases, and ceramide and dihydroceramide are major ceramide species in mammals. In contrast, phytosphingosine (PHS and DHS are the main sphingoid bases in fungi. PHS and phytoceramide exist in particular tissues such as the epidermis in mammals, and involvement of ceramide species in PPARβ activation in cultured keratinocytes has been reported. The purpose of the present study is to investigate whether natural sphingolipids with C18 fatty acid and yeast-derived sphingoid bases activate PPARs as PPAR agonists. Method Lipids of brewer's yeast contain PHS- and DHS-based sphingolipids. To obtain the sphingoid bases, lipids were extracted from brewer's yeast and acid-hydrolyzed. The sphingoid base fraction was purified and quantified. To assess the effects of sphingolipids on PPAR activation, luciferase reporter assay was carried out. NIH/3T3 and human hepatoma (HepG2 cells were transfected with expression vectors for PPARs and retinoid × receptors, and PPAR responsive element reporter vector. When indicated, the PPAR/Gal4 chimera system was performed to enhance the credibility of experiments. Sphingolipids were added to the cells and the dual luciferase reporter assay was performed to determine the transcriptional activity of PPARs. Results We observed that phytoceramide increased the transcriptional activities of PPARs significantly, whereas ceramide and dihydroceramide did not change PPAR activities. Phytoceramide also increased transactivation of

  15. Phytoceramide and sphingoid bases derived from brewer's yeast Saccharomyces pastorianus activate peroxisome proliferator-activated receptors.

    Science.gov (United States)

    Murakami, Itsuo; Wakasa, Yukari; Yamashita, Shinji; Kurihara, Toshio; Zama, Kota; Kobayashi, Naoyuki; Mizutani, Yukiko; Mitsutake, Susumu; Shigyo, Tatsuro; Igarashi, Yasuyuki

    2011-08-24

    Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that regulate lipid and glucose metabolism. PPARα is highly expressed in the liver and controls genes involved in lipid catabolism. We previously reported that synthetic sphingolipid analogs, part of which contains shorter-length fatty acid chains than natural sphingolipids, stimulated the transcriptional activities of PPARs. Sphingosine and dihydrosphingosine (DHS) are abundant sphingoid bases, and ceramide and dihydroceramide are major ceramide species in mammals. In contrast, phytosphingosine (PHS) and DHS are the main sphingoid bases in fungi. PHS and phytoceramide exist in particular tissues such as the epidermis in mammals, and involvement of ceramide species in PPARβ activation in cultured keratinocytes has been reported. The purpose of the present study is to investigate whether natural sphingolipids with C18 fatty acid and yeast-derived sphingoid bases activate PPARs as PPAR agonists. Lipids of brewer's yeast contain PHS- and DHS-based sphingolipids. To obtain the sphingoid bases, lipids were extracted from brewer's yeast and acid-hydrolyzed. The sphingoid base fraction was purified and quantified. To assess the effects of sphingolipids on PPAR activation, luciferase reporter assay was carried out. NIH/3T3 and human hepatoma (HepG2) cells were transfected with expression vectors for PPARs and retinoid × receptors, and PPAR responsive element reporter vector. When indicated, the PPAR/Gal4 chimera system was performed to enhance the credibility of experiments. Sphingolipids were added to the cells and the dual luciferase reporter assay was performed to determine the transcriptional activity of PPARs. We observed that phytoceramide increased the transcriptional activities of PPARs significantly, whereas ceramide and dihydroceramide did not change PPAR activities. Phytoceramide also increased transactivation of PPAR/Gal4 chimera receptors. Yeast-derived sphingoid

  16. Time-varying changes in the simulated structure of the Brewer-Dobson Circulation

    Science.gov (United States)

    Garfinkel, Chaim I.; Aquila, Valentina; Waugh, Darryn W.; Oman, Luke D.

    2017-01-01

    A series of simulations using the NASA Goddard Earth Observing System Chemistry Climate Model are analyzed in order to assess changes in the Brewer-Dobson Circulation (BDC) over the past 55 years. When trends are computed over the past 55 years, the BDC accelerates throughout the stratosphere, consistent with previous modeling results. However, over the second half of the simulations (i.e., since the late 1980s), the model simulates structural changes in the BDC as the temporal evolution of the BDC varies between regions in the stratosphere. In the mid-stratosphere in the midlatitude Northern Hemisphere, the BDC does not accelerate in the ensemble mean of our simulations despite increases in greenhouse gas concentrations and warming sea surface temperatures, and it even decelerates in one ensemble member. This deceleration is reminiscent of changes inferred from satellite instruments and in situ measurements. In contrast, the BDC in the lower stratosphere continues to accelerate. The main forcing agents for the recent slowdown in the mid-stratosphere appear to be declining ozone-depleting substance (ODS) concentrations and the timing of volcanic eruptions. Changes in both mean age of air and the tropical upwelling of the residual circulation indicate a lack of recent acceleration. We therefore clarify that the statement that is often made that climate models simulate a decreasing age throughout the stratosphere only applies over long time periods and is not necessarily the case for the past 25 years, when most tracer measurements were taken.

  17. Global transcription engineering of brewer's yeast enhances the fermentation performance under high-gravity conditions.

    Science.gov (United States)

    Gao, Cuijuan; Wang, Zhikun; Liang, Quanfeng; Qi, Qingsheng

    2010-08-01

    Global transcription engineering was developed as a tool to reprogram gene transcription for eliciting new phenotypes important for technological applications (Science 2006, 314(5805):1565-1568). A recent report indicated that the beneficial growth advantage of yeast cells expressing the SPT15-300 mutation is the result of enhanced uptake and/or improved utilization of leucine and thus was seen only on defined media with low concentrations of leucine (Appl Environ Microbiol 2009, 75(19):6055-6061). Further investigation towards a leucine-prototrophic strain of industrial lager brewer's yeast indicated that integration one copy of SPT15-300 in SPT15 allele, however, did lead to an increased ethanol tolerance on complex rich medium at high gravity fermentation condition. Under brewing conditions, the SPT15-300 mutant produced 80.78 g/L ethanol from 200 g/L carbohydrates after 384 h, almost twice as much as that of the wild-type strain. The results convinced us that the effect of global regulator modification of yeast is at multi-genes level and is extremely complicated.

  18. RELEASE OF INTROGENOUS SUBSTANCES BY BREWER'S YEAST. 3. SHOCK EXCRETION OF AMINO ACIDS.

    Science.gov (United States)

    LEWIS, M J; PHAFF, H J

    1964-06-01

    Lewis, M. J. (University of California, Davis), and H. J. Phaff. Release of nitrogenous substances by brewers' yeast. III. Shock excretion of amino acids. J. Bacteriol. 87:1389-1396. 1964.-When Saccharomyces carlsbergensis (two strains) and S. cerevisiae (one strain) were grown in static culture and the harvested, washed cells were suspended in a solution of glucose, amino acids were suddenly released and then rapidly reabsorbed in a space of about 2 hr. The phenomenon of amino acid release, which was termed shock excretion, varied in intensity with the strain of yeast and was shown to be dependent on the size of the pool of free amino acids within the cells. Shock excretion was independent of osmotic pressure of the suspending medium, but required the presence of a fermentable sugar. d-Galactose and maltose caused shock excretion only when yeast was previously adapted to these sugars. Limiting glucose concentrations prevented reabsorption of amino acids, and a further decrease in glucose concentration also limited excretion. Shock excretion was strikingly reduced when the temperature of the suspending medium was lowered.

  19. Enhanced Brewer Dobson circulation reduces N2O warming potential under climate change

    Science.gov (United States)

    Kracher, Daniela; Reick, Christian; Manzini, Elisa; Schultz, Martin; Stein, Olaf

    2016-04-01

    One implication of climate change is an enhancement of the Brewer Dobson circulation (BDC) triggering the exchange between troposphere and stratosphere. This change in atmospheric dynamics will have effects on atmospheric constituents, especially those with stratospheric sinks such as ozone depleting substances (ODS) including nitrous oxide (N2O). N2O is the most important currently emitted ODS, and the third most important anthropogenic greenhouse gas. Under enhanced BDC, more N2O is transported from the troposphere into the stratosphere, reaching higher altitudes, resulting in an increased N2O sink and a decrease in N2O lifetime. Some aspects of the effect of an enhanced BDC on lifetimes of ODS have already been examined with focus on its implications for ozone. In this study, we examine the effect of a decreasing N2O lifetime in light of climate change. To this end we conduct idealized transient global warming simulations with ECHAM, the atmosphere component of the MPI Earth System Model. As we prescribe surface flux boundary conditions for N2O, we are able to examine further implications of an enhanced N2O sink on atmospheric abundance, which is an important factor for e.g. generating concentration scenarios. Due the idealized simulation setup, we derive findings that are scenario-independent and can easily be extended to other global warming scenarios.

  20. Improved retrieval of nitrogen dioxide (NO2 column densities by means of MKIV Brewer spectrophotometers

    Directory of Open Access Journals (Sweden)

    H. Diémoz

    2014-11-01

    Full Text Available A new algorithm to retrieve nitrogen dioxide (NO2 column densities using MKIV ("Mark IV" Brewer spectrophotometers is described. The method includes several improvements, such as a more recent spectroscopic data set, the reduction of measurement noise, interference by other atmospheric species and instrumental settings, and a better determination of the zenith sky air mass factor. The technique was tested during an ad hoc calibration campaign at the high-altitude site of Izaña (Tenerife, Spain and the results of the direct sun and zenith sky geometries were compared to those obtained by two reference instruments from the Network for the Detection of Atmospheric Composition Change (NDACC: a Fourier Transform Infrared Radiometer (FTIR and an advanced visible spectrograph (RASAS-II based on the differential optical absorption spectrometry (DOAS technique. To determine the extraterrestrial constant, an easily implementable extension of the standard Langley technique for very clean sites without tropospheric NO2 was developed which takes into account the daytime linear drift of stratospheric nitrogen dioxide due to photochemistry. The measurement uncertainty was thoroughly determined by using a Monte Carlo technique. Poisson noise and wavelength misalignments were found to be the most influential contributors to the overall uncertainty, and possible solutions are proposed for future improvements. The new algorithm is backward-compatible, thus allowing for the reprocessing of historical data sets.

  1. Improved retrieval of nitrogen dioxide (NO2 column densities by means of MKIV Brewer spectrophotometers

    Directory of Open Access Journals (Sweden)

    H. Diémoz

    2014-07-01

    Full Text Available A new algorithm to retrieve nitrogen dioxide (NO2 column densities using MKIV Brewer spectrophotometers is described. The method includes several improvements, such as a more recent spectroscopic dataset, the reduction of the measurement noise and interferences by other atmospheric species and instrumental settings, and a better determination of the air mass enhancement factors. The technique was tested during an ad-hoc calibration campaign at the high-altitude site of Izaña (Tenerife, Spain and provided results compatible to those obtained from a spectrometer associated to the Network for the Detection of Atmospheric Composition Change (NDACC, with deviations of less than 0.02 DU. To determine the extraterrestrial constant, an easily implementable generalisation of the standard Langley technique was developed which takes into account the daytime linear drift of nitrogen dioxide due to the photochemistry. Estimates obtained from different observation geometries, by collecting the light from either the sun or the zenith sky, were found to be comparable within the measurement uncertainty. The latter was thoroughly determined by using a Monte Carlo technique. Finally, a method to retrieve additional products such as the degree of linear polarisation of the zenith sky and the oxygen dimer optical depth is presented. The new algorithm is backward-compatible, thus allowing for the reprocessing of historical datasets.

  2. Evaluating the advective Brewer-Dobson circulation in three reanalyses for the period 1979-2012

    Science.gov (United States)

    Abalos, Marta; Legras, Bernard; Ploeger, Felix; Randel, William J.

    2015-08-01

    Most chemistry-climate models show an intensification of the Brewer-Dobson circulation (BDC) in the stratosphere associated with increasing greenhouse gas emissions and ozone depletion in the last decades, but this trend remains to be confirmed in observational data. In this work the evolution of the advective BDC for the period 1979-2012 is evaluated and compared in three modern reanalyses (ERA-Interim, MERRA, and JRA-55). Three different estimates of the BDC are computed for each reanalysis, one based on the definition of the residual circulation and two indirect estimates derived from momentum and thermodynamic balances. The comparison among the nine estimates shows substantial uncertainty in the mean magnitude (˜40%) but significant common variability. The tropical upwelling series show variability linked to the stratospheric quasi-biennial oscillation and to El Niño-Southern Oscillation (ENSO) and also reflect extreme events such as major sudden stratospheric warmings and volcanic eruptions. The trend analysis suggests a strengthening of tropical upwelling of around 2-5%/decade throughout the layer 100-10 hPa. The global spatial structure of the BDC trends provides evidence of an overall acceleration of the circulation in both hemispheres, with qualitative agreement among the estimates. The global BDC trends are mainly linked to changes in the boreal winter season and can be tracked to long-term increases in the resolved wave drag in both hemispheres.

  3. The advective Brewer-Dobson circulation in three reanalyses (1979-2012)

    Science.gov (United States)

    Abalos, Marta; Legras, Bernard; Ploeger, Felix; Randel, William

    2015-04-01

    Most chemistry-climate models predict an intensification of the Brewer-Dobson circulation in the stratosphere in the last decades, but this trend remains to be confirmed in observational data. In this work the evolution of the advective BDC for the period 1979-2012 is evaluated and compared in three modern reanalyses (ERA-Interim, MERRA and JRA-55). Three different estimates of the BDC are computed for each reanalysis, one based on the definition of the residual circulation and two indirect estimates derived from momentum and thermodynamic balance. The comparison among the nine estimates shows substantial uncertainty in the mean magnitude but significant common variability. The trend analysis suggests an intensification in tropical upwelling throughout the layer 100-10 hPa. Globally, an acceleration of the circulation is observed in both hemispheres, with qualitative agreement among the estimates. The global BDC trends are mainly due to changes in the DJF circulation and can be tracked to changes in the resolved wave drag in both hemispheres, which are highly consistent in the three reanalyses.

  4. Modified calibration procedures for a Yankee Environmental System UVB-1 biometer based on spectral measurements with a brewer spectrophotometer.

    Science.gov (United States)

    Vilaplana, José M; Cachorro, Victoria E; Sorribas, Mar; Luccini, Eduardo; de Frutos, Angel M; Berjón, Alberto; de la Morena, Benito

    2006-01-01

    The calibration of the erythemal irradiance measured by a Yankee Environmental System (YES) UVB-1 biometer is presented using two methods of calibration with a wide range of experimental solar zenith angles (SZAs) and ozone values. The calibration is performed through simultaneous spectral measurements by a calibrated double-monochromator Brewer MK-III spectrophotometer at "El Arenosillo" station, located in southwestern Spain. Because the range of spectral measurements of the Brewer spectrophotometer is 290-363 nm, a previously validated radiative transfer model was used to account for the erythemal contribution between 363 and 400 nm. Both methods are recommended by the World Meteorological Organization and we present and discuss here a wide range of results and features given by modified procedures applied to these two general methods. As is well established, the calibration factor for this type of radiometric system is dependent on atmospheric conditions, the most important of which are the ozone content and the SZA. Although the first method is insensitive to these two factors, we analyze this behavior in terms of the range used for the SZA and the use of two different mathematical approaches for its determination. The second method shows the dependence on SZA and ozone content and, thus, a polynomial as a function of SZA or a matrix including SZA and ozone content were determined as general calibration factors for the UV radiometric system. We must note that the angular responses of the YES radiometer and Brewer spectroradiometer have not been considered, because of the difficulty in correcting them. The results show in detail the advantages and drawbacks (and the corresponding associated error) given by the different approaches used for the determination of these calibration coefficients.

  5. Disruption of Brewers' yeast by hydrodynamic cavitation: Process variables and their influence on selective release.

    Science.gov (United States)

    Balasundaram, B; Harrison, S T L

    2006-06-01

    Intracellular products, not secreted from the microbial cell, are released by breaking the cell envelope consisting of cytoplasmic membrane and an outer cell wall. Hydrodynamic cavitation has been reported to cause microbial cell disruption. By manipulating the operating variables involved, a wide range of intensity of cavitation can be achieved resulting in a varying extent of disruption. The effect of the process variables including cavitation number, initial cell concentration of the suspension and the number of passes across the cavitation zone on the release of enzymes from various locations of the Brewers' yeast was studied. The release profile of the enzymes studied include alpha-glucosidase (periplasmic), invertase (cell wall bound), alcohol dehydrogenase (ADH; cytoplasmic) and glucose-6-phosphate dehydrogenase (G6PDH; cytoplasmic). An optimum cavitation number Cv of 0.13 for maximum disruption was observed across the range Cv 0.09-0.99. The optimum cell concentration was found to be 0.5% (w/v, wet wt) when varying over the range 0.1%-5%. The sustained effect of cavitation on the yeast cell wall when re-circulating the suspension across the cavitation zone was found to release the cell wall bound enzyme invertase (86%) to a greater extent than the enzymes from other locations of the cell (e.g. periplasmic alpha-glucosidase at 17%). Localised damage to the cell wall could be observed using transmission electron microscopy (TEM) of cells subjected to less intense cavitation conditions. Absence of the release of cytoplasmic enzymes to a significant extent, absence of micronisation as observed by TEM and presence of a lower number of proteins bands in the culture supernatant on SDS-PAGE analysis following hydrodynamic cavitation compared to disruption by high-pressure homogenisation confirmed the selective release offered by hydrodynamic cavitation.

  6. Towards diacetyl-less brewers' yeast. Influence of ilv2 and ilv5 mutations.

    Science.gov (United States)

    Gjermansen, C; Nilsson-Tillgren, T; Petersen, J G; Kielland-Brandt, M C; Sigsgaard, P; Holmberg, S

    1988-01-01

    During alcoholic fermentations, the off-flavour compound diacetyl is formed non-enzymatically from acetolactate leaking out from the cells. Acetolactate is an intermediate in the biosynthesis of valine. In beer fermentation, the amount of diacetyl is reduced to acceptable levels during maturation. A reduction of the time needed for maturation may be achieved by the use of a brewing yeast that produces less diacetyl. Saccharomyces cerevisiae laboratory strains with an inactive ilv2 gene can not form acetolactate, while ilv5 strains, blocked in the subsequent step, leak acetolactate in high amounts. Induction of recessive mutations in production strains of Saccharomyces carlsbergensis has not yet been achieved, as the yeast is polyploid and possibly a hybrid between S. cerevisiae and another Saccharomyces species. Thus, all chromosomes investigated so far are present in at least two genetically different versions. Genetic and molecular analysis has shown that the brewing yeast is structurally heterozygous for ILV2 and ILV5. Genetic modification of brewers' yeast to reduce diacetyl formation is being carried out by mutation of ILV2. Deletion mutations in both ILV2 alleles have been constructed in vitro to be used for gene replacement in the brewing strain. In addition, partial inactivation of the ILV2 function is carried out by selecting spontaneous dominant mutations resistant to the herbicide sulfometuron methyl. Among these mutants some produce only half the amount of diacetyl compared to the parental strain. An alternative way to reduce diacetyl production might be to increase the activity of the ILV5 gene product. Model experiments in S. cerevisiae show that the presence of the ILV5 gene on a 2-micron based multi-copy vector can reduce the diacetyl production by half.

  7. Encapsulation of brewers yeast in chitosan coated carrageenan microspheres by emulsification/thermal gelation.

    Science.gov (United States)

    Raymond, Marie-Christine; Neufeld, Ronald J; Poncelet, Denis

    2004-05-01

    Brewers yeast was encapsulated in kappa-carrageenan microspheres using an emulsification-thermal gelation approach. Due to heat sensitivity of the yeast at temperatures in excess of 36 degrees C, mixtures of low and high gelation temperature carrageenans were tested to obtain a blend yielding a gelation temperature under 40 degrees C. A 20:80 dispersion of 2% carrageenan sol containing cells, in warm canola oil, produced microspheres upon cooling, with a mean diameter of 450 microm and narrow size dispersion (span of 1.2). Application of a chitosan membrane coat to minimize cell release, increased the mean microsphere diameter to 700 microm, due to the coat thickness and swelling of the microspheres. This diameter was designed so as to minimize mass transfer limitations. Batch fermentations were carried out in a 3 L reactor on a commercial wort medium. Cell loading was 10(7) cells mL(-1) microspheres, and cell "burst" release was observed upon inoculation into fresh medium, whether microspheres were coated or not. The kinetics of intra- and extracapsular cell growth were determined. Increased concentrations of extracapsular free cells could be accounted for by growth in the wort medium, and by ongoing release from the gel microspheres, whether coated or not. Cell release from chitosan-coated carrageenan microspheres was less than that from uncoated microspheres, likely due to retention by the membrane coat. Growth kinetics and alpha-amino nitrogen consumption of encapsulated yeast were higher than that of free cells, and differences in alcohol and ester profiles were also observed, likely due to modified metabolism of the encapsulated yeast.

  8. Substrate activation of brewers' yeast pyruvate decarboxylase is abolished by mutation of cysteine 221 to serine.

    Science.gov (United States)

    Baburina, I; Gao, Y; Hu, Z; Jordan, F; Hohmann, S; Furey, W

    1994-05-10

    Brewers' yeast pyruvate decarboxylase (EC 4.1.1.1), a thiamin diphosphate and Mg(II)-dependent enzyme, isolated from Saccharomyces cerevisiae possesses four cysteines/subunit at positions 69, 152, 221, and 222. Earlier studies conducted on a variant of the enzyme with a single Cys at position 221 (derived from a gene that was the product of spontaneous fusion) showed that this enzyme is still subject to substrate activation [Zeng, X., Farrenkopf, B., Hohmann, S., Jordan, F., Dyda, F., & Furey, W. (1993) Biochemistry 32, 2704-2709], indicating that if Cys was responsible for this activation, it had to be C221. To further test the hypothesis, the C221S and C222S single and the C221S-C222S double mutants were constructed. It is clearly shown that the mutation at C221, but not at C222, leads to abolished substrate activation according to a number of kinetic criteria, both steady state and pre steady state. On the basis of the three-dimensional structure of the enzyme [Dyda, F., Furey, W., Swaminathan, S., Sax, M., Farrenkopf, B., Jordan, F. (1993) Biochemistry 32, 6165-6170], it is obvious that while C221 is located on the beta domain, whereas thiamin diphosphate is wedged at the interface of the alpha and gamma domains, addition of pyruvate or pyruvamide as a hemiketal adduct to the sulfur of C221 can easily bridge the gap between the beta and alpha domains. In fact, residues in one or both domains must be dislocated by this adduct formation. It is very likely that regulation as expressed in substrate activation is transmitted via this direct contact made between the two domains in the presence of the activator.(ABSTRACT TRUNCATED AT 250 WORDS)

  9. [Certain properties of "biosynthetic" L-threonine dehydratase from subcellular structures of brewers' yeast Saccharomyces carlsbergensis].

    Science.gov (United States)

    Kovaleva, S V; Korozhko, A I; Beliaeva, N F; Kagan, Z S

    1981-01-01

    The paper is concerned with kinetic properties of the "biosynthetic" L-threonine dehydratase (EC 4.2.1.16) solubilized from subcellular structures of brewers' yeast Saccharomyces carlsbergensis in the absence and presence of the allosteric inhibitor, L-isoleucine, at three pH-values (pH 6.5, 7.8 and 9.5). The curve of the initial reaction rate versus initial substrate concentration in the absence of L-isoleucine at pH 6.5 was of hyperbolic character (Km = 5.5.10(-2) M), and at pH 7.8 and 9.5 the kinetic curve had a weakly sigmoidal pattern with a sharp going into the saturation plateaux; the values of [S] 0.5 are 1.10(-2) and 8.7.10(-3) M, respectively. An addition of L-isoleucine to the reaction mixture led to the appearance (at pH 6.5) or to an increase (at pH 7.8 and 9.5) of the sigmoidality of these kinetic curves and to a decrease in values of the maximum reaction rate V. The enzyme sensibility to the inhibitory effect of L-isoleucine decreased with an increase in pH values. Low L-isoleucine concentrations at low substrate concentrations activated the enzyme. The pH optimum for L-threonine dehydratase under study was 9.5-10.0. The enzyme molecular weight is about 300 000.

  10. The Brewer-Dobson circulation and total ozone from seasonal to decadal time scales

    Directory of Open Access Journals (Sweden)

    M. Weber

    2011-05-01

    Full Text Available The effect of the winter Brewer-Dobson circulation (BDC on the seasonal and decadal evolution of total ozone in both hemispheres is investigated using satellite total ozone data and outputs from two chemistry-climate models (CCM. Combining data from both hemispheres a linear relationship between the winter cumulative extratropical 100 hPa eddy heat flux and the ozone ratio with respect to fall ozone levels exists and is statistically significant for tropical as well as polar ozone. The high correlation at high latitudes persists well into the summer months until the onset of the next winter season. The anti-correlation of the cumulative eddy heat flux with tropical ozone ratios, however, breaks down in spring as the polar vortex erodes and changes to a weak positive correlation similar to that observed at high latitudes. The inter-annual variability and decadal evolution of ozone in each hemisphere in winter, spring, and summer are therefore driven by the cumulative effect of the previous winter's meridional circulation. This compact linear relationship is also found in two different CCMs (EMAC-FUB, DLR-E39C-A indicating that current models realistically describe the variability in stratospheric circulation and its climate effect on total ozone. Both models show a positive trend in the winter mean eddy heat flux (and winter BDC strength in both hemispheres until year 2050, however the inter-annual variability (peak-to-peak is two to three times larger than the mean change between 1960 and 2050. It is, therefore, possible to detect a shift in this compact linear relationship related to past and future changes in the stratospheric halogen load. A similar shift is difficult to derive from observational data since the satellite era now spanning more than thirty years is still fairly short.

  11. Methane as a Diagnostic Tracer of Changes in the Brewer-Dobson Circulation of the Stratosphere

    Science.gov (United States)

    Remsberg, E. E.

    2015-01-01

    This study makes use of time series of methane (CH4/ data from the Halogen Occultation Experiment (HALOE) to detect whether there were any statistically significant changes of the Brewer-Dobson circulation (BDC) within the stratosphere during 1992-2005. The HALOE CH4 profiles are in terms of mixing ratio versus pressure altitude and are binned into latitude zones within the Southern Hemisphere and the Northern Hemisphere. Their separate time series are then analyzed using multiple linear regression (MLR) techniques. The CH4 trend terms for the Northern Hemisphere are significant and positive at 10 N from 50 to 7 hPa and larger than the tropospheric CH4 trends of about 3%decade(exp -1) from 20 to 7 hPa. At 60 N the trends are clearly negative from 20 to 7 hPa. Their combined trends indicate an acceleration of the BDC in the middle stratosphere of the Northern Hemisphere during those years, most likely due to changes from the effects of wave activity. No similar significant BDC acceleration is found for the Southern Hemisphere. Trends from HALOE H2O are analyzed for consistency. Their mutual trends with CH4 are anti-correlated qualitatively in the middle and upper stratosphere, where CH4 is chemically oxidized to H2O. Conversely, their mutual trends in the lower stratosphere are dominated by their trends upon entry to the tropical stratosphere. Time series residuals for CH4 in the lower mesosphere also exhibit structures that are anti-correlated in some instances with those of the tracer-like species HCl. Their occasional aperiodic structures indicate the effects of transport following episodic, wintertime wave activity. It is concluded that observed multi-year, zonally averaged distributions of CH4 can be used to diagnose major instances of wave-induced transport in the middle atmosphere and to detect changes in the stratospheric BDC.

  12. Variability in the Speed of the Brewer-Dobson Circulation as Observed by Aura/MLS

    Science.gov (United States)

    Flury, Thomas; Wu, Dong L.; Read, W. G.

    2013-01-01

    We use Aura/MLS stratospheric water vapour (H2O) measurements as tracer for dynamics and infer interannual variations in the speed of the Brewer-Dobson circulation (BDC) from 2004 to 2011. We correlate one-year time series of H2O in the lower stratosphere at two subsequent pressure levels (68 hPa, approx.18.8 km and 56 hPa, approx 19.9 km at the Equator) and determine the time lag for best correlation. The same calculation is made on the horizontal on the 100 hPa (approx 16.6 km) level by correlating the H2O time series at the Equator with the ones at 40 N and 40 S. From these lag coefficients we derive the vertical and horizontal speeds of the BDC in the tropics and extra-tropics, respectively. We observe a clear interannual variability of the vertical and horizontal branch. The variability reflects signatures of the Quasi Biennial Oscillation (QBO). Our measurements confirm the QBO meridional circulation anomalies and show that the speed variations in the two branches of the BDC are out of phase and fairly well anti-correlated. Maximum ascent rates are found during the QBO easterly phase. We also find that transport of H2O towards the Northern Hemisphere (NH) is on the average two times faster than to the Southern Hemisphere (SH) with a mean speed of 1.15m/s at 100 hPa. Furthermore, the speed towards the NH shows much more interannual variability with an amplitude of about 21% whilst the speed towards the SH varies by only 10 %. An amplitude of 21% is also observed in the variability of the ascent rate at the Equator which is on the average 0.2mm/s.

  13. Detailed Aerosol Optical Depth Intercomparison between Brewer and Li-Cor 1800 Spectroradiometers and a Cimel Sun Photometer

    Energy Technology Data Exchange (ETDEWEB)

    Cachorro, V. E.; Berjon, A.; Toledano, C.; Mogo, S.; Prats, N.; de Frutos, A. M.; Sorribas, M.; Vilaplana, J. M.; de la Morena, B. A.; Grobner, Julian; Laulainen, Nels

    2009-08-01

    We present here representative results about a comparison of aerosol optical depth (AOD) using different instruments during three short and intensive campaigns carried out from 1999 to 2001 at El Arenosillo (Huelva, Spain). The specific aim of this study is to determine the level of agreement between three different instruments operating at our station. This activity, however, is part of a broader objective to recover an extended data series of AOD in the UV range obtained from a Brewer spectroradiometer. This instrument may be used to obtain AOD at the same five UV wavelengths used during normal operation for ozone content determination. As part of the validation of the Brewer AOD data recovery process, a Cimel sun photometer and another spectroradiometer, a Licor1800, were used. The Licor1800 spectroradiometer (which covers the spectral range 300-1100 nm) was the first instrument used at this station for aerosol monitoring (1996-99) and it was operated during these intercomparison campaigns (1999-2001) specifically to assess the continuity of the AOD data series. The Cimel sunphotometer was installed at our station at the beginning of 2000 as part of AERONET to provide AOD data over the visible and near infrared spectrum. A detailed comparison of these three instruments is carried out by means of near-simultaneous measurements, with particular emphasis on examining any diurnal AOD variability that may be linked with calibration and/or measurement errors or real atmospheric variability. Because the comparison is carried out from UV (320nm) to near infrared (1020nm) wavelengths under all possible atmospheric conditions (including clouds), AOD values range from near zero up to 1. Absolute AOD uncertainties range from 0.02 for the Cimel to 0.08 for the Brewer, with intermediate values for the Licor1800. All the values during the comparison are in reasonable agreement, when taking into account the different performance characteristics of each instrument. The

  14. Aerosol Optical Depth measurements at 340 nm with a Brewer spectrophotometer and comparison with Cimel sunphotometer observations at Uccle, Belgium

    Directory of Open Access Journals (Sweden)

    V. De Bock

    2010-11-01

    Full Text Available The Langley Plot Method (LPM is adapted for the retrieval of Aerosol Optical Depth (AOD values at 340 nm from Brewer#178 sun scan measurements between 335 and 345 nm (convoluted with the band pass function of the Cimel sunphotometer filter at 340 nm performed in Uccle, Belgium. The use of sun scans instead of direct sun measurements simplifies the comparison of the AOD values with quasi-simultaneous Cimel sunphotometer values. Also, the irradiance at 340 nm is larger than the one at 320.1 nm due to lower ozone absorption, thus improving the signal to noise ratio. For the selection of the cloudless days (from now on referred to as calibration quality clear days, a new set of criteria is proposed. With the adapted method, individual clear sky AOD values, for which the selection criteria are also presented in this article, are calculated for a period from September 2006 until the end of August 2010. These values are then compared to quasi-simultaneous Cimel sunphotometer measurements, showing a very good agreement (the correlation coefficient, the slope and the intercept of the regression line are respectively 0.974, 0.968 and 0.011, which proves that good quality observations can be obtained from Brewer sun scan measurements at 340 nm. The analysis of the monthly and seasonal Brewer AODs at Uccle is consistent with studies at other sites reporting on the seasonal variation of AODs in Europe. The highest values can be observed in summer and spring, whereas more than 50% of the winter AODs are lower than 0.3. On a monthly scale, the lowest AOD are observed in December and the highest values occur in June and April. No clear weekly cycle is observed for Uccle. The current cloud-screening algorithm is still an issue, which means that some AOD values can still be influenced by scattered clouds. This effect can be seen when comparing the calculated monthly mean values of the Brewer with the AERONET measurements.

  15. Growth and nitrogen metabolism of sea bass fed graded levels of nucleic acid nitrogen from yeast or RNA extract as partial substitute for protein nitrogen from fish meal

    Directory of Open Access Journals (Sweden)

    S. Kaushik

    2010-01-01

    Full Text Available Some studies carried out in mammalian models have shown de novo synthesis and salvage of nucleotides to be a costly metabolic process and a dietary supplementation with nucleic acids (NA or nucleotides has been suggested to result in a protein sparing action (Sanderson and He, 1994. On the other hand, high levels of dietary NA could have toxic effects and lead to disturbance in protein, lipid and carbohydrate metabolism in monogastric animals lacking uricase activity, an enzyme involved in NA degradation (Clifford and Story, 1976. So far, there is no clear indication of such effects in fish fed nucleic acid-enriched diets (Tacon and Cooke, 1980; Rumsey et al., 1992; Fournier et al., 2002. The aim of this experiment was to investigate growth response and N metabolism in juvenile sea bass (D. labrax fed diets supplying graded levels of nucleic acid N from dry brewer's yeast or RNA extract as partial substitutes for protein nitrogen provided by fish meal.

  16. Microbiota of kefir grains

    Directory of Open Access Journals (Sweden)

    Tomislav Pogačić

    2013-03-01

    Full Text Available Kefir grains represent the unique microbial community consisting of bacteria, yeasts, and sometimes filamentous moulds creating complex symbiotic community. The complexity of their physical and microbial structures is the reason that the kefir grains are still not unequivocally elucidated. Microbiota of kefir grains has been studied by many microbiological and molecular approaches. The development of metagenomics, based on the identification without cultivation, is opening new possibilities for identification of previously nonisolated and non-identified microbial species from the kefir grains. Considering recent studies, there are over 50 microbial species associated with kefir grains. The aim of this review is to summarise the microbiota composition of kefir grains. Moreover, because of technological and microbiological significance of the kefir grains, the paper provides an insight into the microbiological and molecular methods applied to study microbial biodiversity of kefir grains.

  17. The Fed's Year of Transition

    Science.gov (United States)

    Schug, Mark C.; Niederjohn, Scott

    2006-01-01

    The purpose of this article is to: (1) Examine the historical development of the Federal Reserve System; (2) Provide background on Ben Bernanke, the new Fed chairman; (3) Explain the basic tools of monetary policy used by the Fed; (4) Examine the causes of the Great Depression, a topic of special interest to Bernanke; and (5) Provide some key…

  18. Grain Accumulation of Selenium Species in Rice (Oryza sativa L.)

    Energy Technology Data Exchange (ETDEWEB)

    Carey, Anne-Marie; Scheckel, Kirk G.; Lombi, Enzo; Newville, Matt; Choi, Yongseong; Norton, Gareth J.; Price, Adam H.; Meharg, Andrew A. (EPA); (U. South Australia); (Aberdeen); (UC)

    2012-09-05

    Efficient Se biofortification programs require a thorough understanding of the accumulation and distribution of Se species within the rice grain. Therefore, the translocation of Se species to the filling grain and their spatial unloading were investigated. Se species were supplied via cut flag leaves of intact plants and excised panicle stems subjected to a {+-} stem-girdling treatment during grain fill. Total Se concentrations in the flag leaves and grain were quantified by inductively coupled plasma mass spectrometry. Spatial accumulation was investigated using synchrotron X-ray fluorescence microtomography. Selenomethionine (SeMet) and selenomethylcysteine (SeMeSeCys) were transported to the grain more efficiently than selenite and selenate. SeMet and SeMeSeCys were translocated exclusively via the phloem, while inorganic Se was transported via both the phloem and xylem. For SeMet- and SeMeSeCys-fed grain, Se dispersed throughout the external grain layers and into the endosperm and, for SeMeSeCys, into the embryo. Selenite was retained at the point of grain entry. These results demonstrate that the organic Se species SeMet and SeMeSeCys are rapidly loaded into the phloem and transported to the grain far more efficiently than inorganic species. Organic Se species are distributed more readily, and extensively, throughout the grain than selenite.

  19. Identification of Sc-type ILV6 as a target to reduce diacetyl formation in lager brewers' yeast.

    Science.gov (United States)

    Duong, C T; Strack, L; Futschik, M; Katou, Y; Nakao, Y; Fujimura, T; Shirahige, K; Kodama, Y; Nevoigt, E

    2011-11-01

    Diacetyl causes an unwanted buttery off-flavor in lager beer. It is spontaneously generated from α-acetolactate, an intermediate of yeast's valine biosynthesis released during the main beer fermentation. Green lager beer has to undergo a maturation process lasting two to three weeks in order to reduce the diacetyl level below its taste-threshold. Therefore, a reduction of yeast's α-acetolactate/diacetyl formation without negatively affecting other brewing relevant traits has been a long-term demand of brewing industry. Previous attempts to reduce diacetyl production by either traditional approaches or rational genetic engineering had different shortcomings. Here, three lager yeast strains with marked differences in diacetyl production were studied with regard to gene copy numbers as well as mRNA abundances under conditions relevant to industrial brewing. Evaluation of data for the genes directly involved in the valine biosynthetic pathway revealed a low expression level of Sc-ILV6 as a potential molecular determinant for low diacetyl formation. This hypothesis was verified by disrupting the two copies of Sc-ILV6 in a commercially used lager brewers' yeast strain, which resulted in 65% reduction of diacetyl concentration in green beer. The Sc-ILV6 deletions did not have any perceptible impact on beer taste. To our knowledge, this has been the first study exploiting natural diversity of lager brewers' yeast strains for strain optimization.

  20. Performance and Carcass Characteristics of Rabbits Fed Oil Supplemented Diets

    Directory of Open Access Journals (Sweden)

    Jamal Abo OMAR

    2016-02-01

    Full Text Available The objective of this study is to investigate the effects of feeding different dietary fat supplements in the finisher rations of Baladi rabbits, including sesame oil (SO, olive oil sediments (OOS, and poultry grease (PG, in comparison to the traditional oil supplement, the soybean soap stock oil (SS, on growth performance, blood lipid profile, dressing percentage and carcass cut, and meat quality: water holding capacity (WHC and cell forming unit (CFU. A total of 48 Baladi rabbits were used, with individual body weights (BW of 519 ± 22 g at the beginning of the experiment. Rabbits were randomly divided into 4 groups of 12, and those in each group were individually fed cereal grain-soybean meal (SBM with a fixed amount (i.e. 30 g/kg dry matter (DM of oil, being either soybean oil (SOY, olive oil sediments (OOS, recycled restaurant oil (RRO, or poultry grease (PG. All rations were isonitrogenous and contained iso-metabolizable energy (ME. At the end of the 44 day feeding trial, all animals were slaughtered. Rabbits fed a SOY supplemented diet consumed more (P < 0.05 feed than those fed the OOS, RRO, or PG supplemented diets. However, rabbits fed the SOY had a better (P < 0.05 feed conversion ratio than rabbits fed the OOS, PG, or RRO diets. Oil source had no effect on carcass components weights. Liver was heavier (P < 0.05 in rabbits fed the SOY supplemented diet. However, the RRO fed rabbits had heavier (P < 0.05 small intestine, large intestine and cecum. In conclusion, the positive effects of the tested oil supplements (i.e. RRO, OOS, PG on the studied performance and carcass traits is encouraging, but more investigation is needed to identify the optimal levels for these supplements in various diets of local rabbits.

  1. Composição química e perfil de ácidos graxos da carne de bovinos de diferentes condições sexuais recebendo silagem de milho e concentrado ou cana-de-açúcar e concentrado contendo grãos de girassol Chemical traits and fatty acids composition of beef from young bulls, steers and heifers fed corn silage and concentrate or sugarcane and concentrate with sunflower grains

    Directory of Open Access Journals (Sweden)

    Alexandre Rodrigo Mendes Fernandes

    2009-04-01

    Full Text Available O objetivo neste trabalho foi avaliar as características químicas e a composição em ácidos graxos do contrafilé (músculo Longissimus de tourinhos, novilhos e novilhas da raça Canchim. Os animais foram terminados em confinamento com duas dietas experimentais, uma com silagem de milho e concentrado e outra com cana-de-açúcar e concentrado contendo grãos de girassol. Os teores de umidade, proteína e minerais no músculo não diferiram entre as dietas e as condições sexuais dos animais. A carne de novilhos terminados com a dieta com grãos de girassol apresentou maior teor de lipídios (3,31% em comparação à dos animais de outras categorias. Os animais que receberam a dieta com grãos de girassol apresentaram maiores concentrações de ácido linoléico conjugado (18:2 cis9, trans-11 (0,73% e ácidos graxos poliinsaturados (8,12% no músculo, e também relações mais elevadas de ácidos graxos insaturados:saturados (0,93 e ácidos graxos poliinsaturados:saturados (0,16 em comparação àqueles que receberam a dieta convencional, à base de silagem de milho (0,34%; 6,31%; 0,86; e 0,11, respectivamente. A composição em ácidos graxos da carne de bovinos pode ser melhorada com a utilização de cana-de-açúcar e grãos de girassol na dieta dos animais terminados em confinamento.The objective in this work was to evaluate the chemical characteristics and the fatty acids composition of beef loin (Longissimus muscle of young bulls, steers and heifers of Canchim breed. The animals were finished in feedlot system and fed two experimental diets, one with corn silage and concentrate (SIL and the other with sugar cane, as exclusive roughage, and concentrate containing sunflower grains (CNA. Differences were not detected in relation to moisture, protein and mineral contents between diets or sexual categories. The meat from steers that received the CNA diet had higher lipid content (3.31% in relation to meat from young bulls and heifers

  2. Against the Grain

    DEFF Research Database (Denmark)

    Pedersen, Peter Ole

    2016-01-01

    Katalog-tekst til udstillingen Against the Grain om atomar-fotografi og det antropocæne. Kurateret af Peter Ole Pedersen på Galleri Image, august-oktober 2016.......Katalog-tekst til udstillingen Against the Grain om atomar-fotografi og det antropocæne. Kurateret af Peter Ole Pedersen på Galleri Image, august-oktober 2016....

  3. The primary structure of tRNAIIArg from brewers' yeast. 1. Complete digestions with pancreatic and T1 ribonucleases.

    Science.gov (United States)

    Weissenbach, J; Martin, R; Dirheimer, G

    1975-08-15

    tRNAIIArg purified from bulk brewers' yeast tRNA by countercurrent distribution followed by two column-chromatographic steps was completely digested with pancreatic and T1 ribonucleases. Isolations of the products have been carried out either by column chromatography or by high-voltage electrophoresis. Analyses of the isolated nucleotides and olignoucleotides were in good agreement and indicate that this tRNA is composed of 76 nucltotide residues including 13 minor nucleotides. Overlaps resulting from the end-products of the two complementary digests led to a sequence of 25 residues. The primary structure of tRNAIIArg has been determined after partial digestion with T1 ribonuclease as described in the following paper.

  4. Brewer's yeast cell wall affects microbiota composition and decreases Bacteroides fragilis populations in an anaerobic gut intestinal model.

    Science.gov (United States)

    Nakashimada, Yutaka; Michinaka, Atsuko; Watanabe, Kentaro; Nishio, Naomichi; Fujii, Toshio

    2011-02-01

    Brewer's yeast cell wall (BYC) has been reported to have prebiotic activity that improves the microbiotal composition of the human gut. To understand the precise effect of BYC on gut microbiota and its metabolism, we used a three-stage continuous-flow reactor system that mimicked the environment of the large intestine. The reactor system was able to maintain the bacterial community stably for a week. The Bacteroides fragilis population decreased drastically after the addition of BYC into this system while the number of Lactobacillus was stably maintained. In addition, propionate and acetate levels increased drastically. This metabolic change correlated with an increase in a number of specific operational taxonomic units annotated to the genus Veillonella and Megasphaella. These results suggest that BYC affects the composition of microbiota in an in vitro model system. Copyright © 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  5. GrainSpotter

    DEFF Research Database (Denmark)

    Schmidt, Søren

    2014-01-01

    A new approach for indexing multigrain diffraction data is presented. It is based on the use of a monochromatic beam simultaneously illuminating all grains. By operating in sub-volumes of Rodrigues space, a powerful vertex-finding algorithm can be applied, with a running time that is compatible...... with online analysis. The resulting program, GrainSpotter, is sufficiently fast to enable online analysis during synchrotron sessions. The program applies outlier rejection schemes, leading to more robust and accurate data. By simulations it is shown that several thousand grains can be retrieved. A new method...... to derive partial symmetries, called pseudo-twins, is introduced. Uniquely, GrainSpotter includes an analysis of pseudo-twins, which is shown to be critical to avoid erroneous grains resulting from the indexing....

  6. Physics of Grain Alignment

    CERN Document Server

    Lazarian, A

    2000-01-01

    Aligned grains provide one of the easiest ways to study magnetic fields in diffuse gas and molecular clouds. How reliable our conclusions about the inferred magnetic field depends critically on our understanding of the physics of grain alignment. Although grain alignment is a problem of half a century standing recent progress achieved in the field makes us believe that we are approaching the solution of this mystery. I review basic physical processes involved in grain alignment and show why mechanisms that were favored for decades do not look so promising right now. I also discuss why the radiative torque mechanism ignored for more than 20 years looks right now the most powerful means of grain alignment.

  7. Methane emissions from feedlot cattle fed barley or corn diets.

    Science.gov (United States)

    Beauchemin, K A; McGinn, S M

    2005-03-01

    Methane emitted from the livestock sector contributes to greenhouse gas emissions worldwide. Understanding the variability in enteric methane production related to diet is essential to decreasing uncertainty in greenhouse gas emission inventories and to identifying viable greenhouse gas reduction strategies. Our study focused on measuring methane in growing beef cattle fed corn- or barley-based diets typical of those fed to cattle in North American feedlots. The experiment was designed as a randomized complete block (group) design with two treatments, barley and corn. Angus heifer calves (initial BW = 328 kg) were allocated to two groups (eight per group), with four cattle in each group fed a corn or barley diet. The experiment was conducted over a 42-d backgrounding phase, a 35-d transition phase and a 32-d finishing phase. Backgrounding diets consisted of 70% barley silage or corn silage and 30% concentrate containing steam-rolled barley or dry-rolled corn (DM basis). Finishing diets consisted of 9% barley silage and 91% concentrate containing barley or corn (DM basis). All diets contained monensin (33 mg/kg of DM). Cattle were placed into four large environmental chambers (two heifers per chamber) during each phase to measure enteric methane production for 3 d. During the backgrounding phase, DMI was greater by cattle fed corn than for those fed barley (10.2 vs. 7.6 kg/d, P cattle were in the chambers; thus, methane emissions (g/d) reported may underestimate those of the feedlot industry. Methane emissions per kilogram of DMI and as a percentage of GE intake were not affected by grain source during the backgrounding phase (24.6 g/kg of DMI; 7.42% of GE), but were less (P methane emissions of cattle fed high-forage backgrounding diets and barley-based finishing diets. Mitigating methane losses from cattle will have long-term environmental benefits by decreasing agriculture's contribution to greenhouse gas emissions.

  8. Comparison of UV irradiances from Aura/Ozone Monitoring Instrument (OMI with Brewer measurements at El Arenosillo (Spain – Part 1: Analysis of parameter influence

    Directory of Open Access Journals (Sweden)

    M. Antón

    2010-07-01

    Full Text Available The main objective of this study is to compare the erythemal UV irradiance (UVER and spectral UV irradiances (at 305, 310 and 324 nm from the Ozone Monitoring Instrument (OMI onboard NASA EOS/Aura polar sun-synchronous satellite (launched in July 2004, local equator crossing time 01:45 p.m. with ground-based measurements from the Brewer spectrophotometer #150 located at El Arenosillo (South of Spain. The analyzed period comprises more than four years, from October 2004 to December 2008. The effects of several factors (clouds, aerosols and the solar elevation on OMI-Brewer comparisons were analyzed. The proxies used for each factor were: OMI Lambertian Equivalent Reflectivity (LER at 360 nm (clouds, the aerosol optical depth (AOD at 440 nm measured from the ground-based Cimel sun-photometer (http://aeronet.gsfc.nasa.gov, and solar zenith angle (SZA at OMI overpass time. The comparison for all sky conditions reveals positive biases (OMI higher than Brewer 12.3% for UVER, 14.2% for UV irradiance at 305 nm, 10.6% for 310 nm and 8.7% for 324 nm. The OMI-Brewer root mean square error (RMSE is reduced when cloudy cases are removed from the analysis, (e.g., RMSE~20% for all sky conditions and RMSE smaller than 10% for cloud-free conditions. However, the biases remain and even become more significant for the cloud-free cases with respect to all sky conditions. The mentioned overestimation is partially due to aerosol extinction influence. In addition, the differences OMI-Brewer typically decrease with SZA except days with high aerosol loading, when the bias is near constant. The seasonal dependence of the OMI-Brewer difference for cloud-free conditions is driven by aerosol climatology.

    To account for the aerosol effect, a first evaluation in order to compare with previous TOMS results (Antón et al., 2007 was performed. This comparison shows that the OMI bias is between +14% and +19% for

  9. Performance of organic grain legumes in Tuscany

    Directory of Open Access Journals (Sweden)

    Valentina Moschini

    2014-03-01

    Full Text Available In 2005-2007 growing season, few varieties of field bean, high protein pea and white lupin were compared in an organic farm of Central Italy (Mugello area, Tuscany, to evaluate their agronomic performance in terms of grain yield, nutritional quality and competitive ability against weeds. The experiment was performed under rain-fed conditions. Furthermore, grain legumes features were compared between two different sowing seasons (autumnal vs late-winter for two years, in order to get information on the best time of sowing of these species, and the stability of yields of different genotypes in those climatic and soil conditions. These legumes could be an alternative protein source to external soybean, a high-risk alimentary source of genetically modified organisms, in the organic livestock sector. The main findings indicate that higher yields in grain and crude protein were obtained with the pea species and in particular with cultivars Hardy (4.0 t/ha grain yield; 626 kg/ha crude protein yield and Classic (3.1 t/ha grain yield; 557 kg/ha crude protein yield; followed by field bean cv. Chiaro di Torre Lama (2.9 t/ha grain yield; 624 kg/ha crude protein yield and cv. Vesuvio (2.5 t/ha grain yield; 549 kg/ha crude protein yield. Furthermore the field bean is interesting for the stability of yield in both years despite climatic conditions rather different. The white lupin has showed the lower yield but the best values of grain quality, with higher values in lupin Multitalia for dry matter, crude protein and ether extract and in lupin Luxe also for crude fibre, respect to the other legumes analysed. Among lupin varieties, lupin Multitalia showed the best yield results for the pedo-climatic conditions of Mugello area (0.9 t/ha lupin Multitalia; 0.2 t/ha lupin Luxe. The total yield of organic grain legumes, in the experimental site, is resulted higher with an autumnal seeding respect to the late-winter seeding (2.8 t/ha vs 1.9 t/ha.

  10. Folding of Pollen Grains

    Science.gov (United States)

    Katifori, Eleni; Alben, Silas; Cerda, Enrique; Nelson, David; Dumais, Jacques

    2008-03-01

    At dehiscence, which occurs when the anther reaches maturity and opens, pollen grains dehydrate and their volume is reduced. The pollen wall deforms to accommodate the volume loss, and the deformation pathway depends on the initial turgid pollen grain geometry and the mechanical properties of the pollen wall. We demonstrate, using both experimental and theoretical approaches, that the design of the apertures (areas on the pollen wall where the stretching and the bending modulus are reduced) is critical for controlling the folding pattern, and ensures the pollen grain viability. An excellent fit to the experiments is obtained using a discretized version of the theory of thin elastic shells.

  11. Trânsito gastrintestinal e digestibilidade aparente de nutrientes em eqüinos alimentados com dietas contendo grãos secos ou silagem de grãos úmidos de triticale Gastrointestinal transit and apparent digestibility of nutrients in equines fed with diets containing dry or high-moisture triticale grain silage

    Directory of Open Access Journals (Sweden)

    Kátia de Oliveira

    2007-12-01

    kg BW were allotted to latin square experimental design. The objective was to evaluate the utilization of diets containing triticale grains (dry or ensiled in substitution of corn in the feeding of equine feeding, using assay of gastrointestinal transit and digestibility. The treatments consisted of three levels of triticale grains in substitution of corn grains (0, 50 and 100%, and two conservation forms of triticale grains (dry or ensiled. The diets were isoproteic (12.5% CP, with aDM intake by the animals of 2,0% BW (forage to concentrate 50:50. The apparent coefficients of digestibility (aCD of the DM, OM, CP and NDF were determined by indirect method. The parameters of the gastrointestinal transit were: k1 (passage rate in the large intestine; k2 (passage rate in the stomach; TT (transit time; TMR (mean time retention and TMRT (mean total retention time. No difference was observed for aCD of DM, OM, CP and NDF among the experimental diets, with means values of 64.31, 65.14, 74.13 and 57.25%, respectively. Considering the kinetics of the solid and liquid phases, it was found effect only to the k2 for the solid phase of digesta, whose values in the diets containing 100% triticale (dry or ensiled was of 19.63%/h and the control diet of 23.72%/h. An increasing linear effect was observed for TT in the solid phase of digesta, as the levels of substitution of corn grain by ensiled triticale grain increased. In conclusion, the concentrates for equines may be formulated with dry or ensiled triticale grains in total substitution of corn grains. The inclusion of the triticale grains in the equine feeding promoted slow transit of the digesta.

  12. The influence of taste in willingness-to-pay valuations of sirloin steaks from postextraction algal residue-fed cattle.

    Science.gov (United States)

    Johnson, M D; Miller, R K; Morrill, J C; Anderson, D P; Wickersham, T A; Sawyer, J E; Richardson, J W; Palma, M A

    2016-07-01

    Consumer preferences and willingness-to-pay (WTP) for beef sirloin steaks with differing production, physical, and credence attributes related to the use of postextraction algal residue (PEAR), a novel feed ingredient, were estimated. Ninety-six consumers participated in a sensory tasting panel before completing a choice set survey; 127 consumers completed only the choice set survey without sampling products. Steaks from grain- and PEAR-fed steers had similar Warner-Bratzler shear force (WBSF) scores (1.89 kg and 2.01 kg, respectively; = 0.77) and had lower WBSF scores than steaks from grass-fed steers (3.37 kg; 0.26). Panelists rated the juiciness like/dislike of steaks from PEAR-fed cattle the highest ( < 0.01) among the 3 samples. Sensory tasting of the products was observed to alter the preferences of consumers. Consumers who completed only the survey negatively perceived beef from PEAR-fed cattle compared with beef from grain-fed cattle, with a WTP discount of -US$1.17/kg. However, with sensory tasting, the WTP for beef from PEAR-fed cattle was not discounted relative to beef from grain-fed cattle ( = 0.21). The nontasting consumers had much higher stated WTP values for credence attributes. Factors that influence the eating experience (tenderness and quality grade) dominated as the most important attributes on WTP among the tasting group. The use of no hormones and no antibiotics in production had a premium of $2.34/kg among the nontasting group, but with tasting, the premium was $1.19/kg. If PEAR-fed beef came to market, there would be no need to differentiate it from grain-fed beef unless retailers wanted to market it as a differentiated product. If it were marketed as a differentiated product, retailers would need to hold promotional tastings to change consumer's preconceived notions about the product.

  13. 蛋白酶水解啤酒糟蛋白动力学研究%Reaction kinetics of the hydrolysis of brewer's spent grains protein with protease

    Institute of Scientific and Technical Information of China (English)

    宗绪岩; 刘长江; 李丽; 刘玲; 宣丽; 许金光

    2009-01-01

    为了研究蛋白酶水解啤酒糟蛋白的动力学性质,采用pH-stat法,以蛋白酶对啤酒糟中提取的蛋白进行水解处理.探讨了底物浓度[S]、酶与底物浓度比[E]/[S]和反应时间t对产物水解度DH的影响.建立了[S]及t同DH间的数学模型.通过不同底物浓度加碱量随水解时间的变化速度,测定出蛋白酶水解啤酒糟蛋白的K_m值.

  14. 啤酒糟蛋白水解液脱色工艺优化研究%Optimization of Decoloring Technique of Hydrolysis of Brewer's Spent Grains Protein

    Institute of Scientific and Technical Information of China (English)

    宗绪岩; 刘长江; 刘玲; 李丽; 许金光

    2009-01-01

    为了优化啤洒糟蛋白水解液脱色工艺,并使其得到更好的应用,考察了不同的脱色剂对脱色效果的影响,并通过正交试验优化了活性炭脱色条件.结果表明:颗粒状活性炭脱色效果较优;在活性炭脱色试验中脱色率和肽氮类物质的损失率的变化与各影响因素都表现为正相关;各因素对于脱色率的影响顺序为:添加量>脱色温度>脱色时间;对于肽氮类物质损失率的影响顺序为:添加量>脱色时间>脱色温度.较优的脱色条件为添加4%的颗粒状活性炭50%下脱色2.5h,脱色率达到36.72%,肽氮类物质损失率为20.96%.

  15. Experiment on Ferment of Brewers Spent Grains by Combining Solid Mold%混菌固态发酵啤酒糟的研究

    Institute of Scientific and Technical Information of China (English)

    全艳玲; 解生权; 孟素华

    2002-01-01

    利用不同原料配比进行混菌发酵啤酒糟来提高其蛋白质量分数研究.研究表明,利用木霉、黑曲霉和酵母混菌发酵,啤酒糟和麦夫皮配比为4∶1时,在28-30 ℃,含水量65%-70%条件下,发酵3 d后蛋白质质量分数提高到35%以上.

  16. Studies on Extraction of Protein from Brewer's Spent Grain by Alcohol-alkali Method%醇-碱法提取啤酒糟中蛋白质

    Institute of Scientific and Technical Information of China (English)

    李志东; 李娜; 魏丽

    2008-01-01

    以酒糟为原料,通过醇-碱法进行蛋白质提取.对影响蛋白质提取的因素,如提取剂醇-碱体积比和用量的选择、提取温度、提取时间等条件进行研究,并进行了正交试验.确定了获得最大提取量的条件为醇-碱体积比1∶2的混合液作为提取剂,每10 g干酒糟加入30 mL;提取温度30 ℃,时间70 min.

  17. Manufacturing beverages from dietary fiber of brewer's spent grains%啤酒糟膳食纤维饮料的研制

    Institute of Scientific and Technical Information of China (English)

    王金华; 郭健

    2003-01-01

    以啤酒糟为原料提取可溶性膳食纤维与脱脂乳混合,接入乳酸菌发酵.经调配、均质等工艺过程,制成一种功能性乳酸发酵纤维饮料,为啤酒糟的开发利用提供了一条新的有效途径.

  18. Study On Hydrolyzing Protein in Brewer's Spent Grains%酶法水解啤酒糟提取蛋白质的研究

    Institute of Scientific and Technical Information of China (English)

    吴会丽; 王异静; 张丽叶

    2006-01-01

    研究了采用酶解法提取啤酒糟中蛋白质的工艺条件,通过正交试验得到提取的最佳工艺条件为:水解蛋白酶的添加量2mL/100g干啤酒糟,反应温度60℃,pH8.0,反应时间5h,固液比1:12.在此条件下,水解蛋白提取率为63.6%.采用高效液相法对酶解液中的18种氨基酸含量进行了分析.18种游离氨基酸含量占总蛋白含量的24%,8种游离状态的必需氨基酸占游离氨基酸的39%.

  19. 生物技术在啤酒糟综合利用中的应用%Comprehensive Utilization of Biotechnology in Brewer's Spent Grain

    Institute of Scientific and Technical Information of China (English)

    邱峰; 李志东; 李娜; 费天宇

    2007-01-01

    我国每年约产50万吨的啤酒糟,对啤酒糟进行生物发酵可以生产蛋白饲料、粗酶制剂、复合氨基酸、甘油、酒精和沼气等物质.综合利用啤酒糟可以开发出多种产品,使啤酒糟资源化,减轻环境污染.

  20. 啤酒糟蛋白提取模型的建立%Model of extraction of brewer's spent grain protein

    Institute of Scientific and Technical Information of China (English)

    刘长江; 宗绪岩; 刘玲; 李丽; 许金光

    2010-01-01

    为了更好地利用啤酒糟中含有的蛋白成分,研究了不同提取条件对糟蛋白提取率的影响,并利用二次多项式回归的方法建立了啤酒糟蛋白提取率与提取条件之间关系的数学模型,并探讨了因素相互间的影响和产生的原因.结果表明:利用岭脊分析的方法得到较佳的提取条件为pH 10.5,液料比15.0:1,45.7℃提取59.9min.通过方差分析和判定系数检验,证明该模型拟合度较好.

  1. Development of Instant Nutritious Paste Produced by Brewer's Spent Grains%啤酒糟复合即食营养糊的研制

    Institute of Scientific and Technical Information of China (English)

    李丽; 宗绪岩; 张静; 王雯婷

    2013-01-01

    为了合理利用啤酒糟,对啤酒糟的处理方法、原料配比及膨化条件进行了研究.结果表明,添加15%的啤酒糟、15%的大豆、42%的玉米和28%的大米,经粉碎、混合后调整水分含量为15%,在膨化温度140℃条件下膨化,粉碎后过100目筛,生产的啤酒糟复合即食营养糊具有较好的感官质量.

  2. 利用啤酒麦糟培养木霉条件的研究%Study on The Cultural Condition of Trichoderma Koningll on Brewer's Spent Grains

    Institute of Scientific and Technical Information of China (English)

    孙玉梅; 王培忠; 何连芳; 曹方; 张莉; 姜铁; 王明辉

    2001-01-01

    该文研究了利用啤酒麦糟培养木霉以获得单细胞蛋白饲料,适宜培养条件为:在麦糟中加入0.5%(NH4)2SO4和0.2%CaCl2,在30℃恒温培养4~5天,在此条件下的培养物粗蛋白含量可达29%(干基)以上.

  3. 啤酒糟和麸皮混贮品质分析%Analysis of Mixed Silage Quality of Brewers Spent Grains(BSG) and Bran

    Institute of Scientific and Technical Information of China (English)

    时建青; 徐红蕊; 艾尼瓦尔艾山; 赵国琦

    2005-01-01

    将啤酒糟和麸皮按不同比例袋装混贮,30 d开袋,取样进行感官评定测定氨态氮含量、pH值、干物质(DM)、粗蛋白(CP)、中性和酸性洗涤纤维(NDF和ADF)及进行有机酸含量分析.结果水分含量对混贮效果有显著影响,以袋装3 kg和4 kg混贮原料含水量分别为50%和60%的啤酒糟和麸皮混贮效果最好,其次是40%,70%最差.

  4. Ultrasound-assisted Extraction of Protein from Brewer's Spent Grain%超声辅助萃取啤酒糟中的蛋白质

    Institute of Scientific and Technical Information of China (English)

    唐德松; 尹刚民; 李冰; 李琳

    2010-01-01

    本文采用超声辅助萃取浸提啤酒糟中的蛋白质.实验对几种浸提溶剂进行筛选,采用碳酸钠缓冲液可以获得较好的浸提效果;对啤酒糟进行粉碎处理可以比原啤酒糟获得更高的蛋白质得率.与传统提取方法(摇床浸提)相比,超声辅助萃取可以提高传质速率.在超声辅助萃取过程中,蛋白质的得率与萃取时间紧密相关,浸提时间在60 min之前,溶剂中的蛋白质浓度随着时间快速升高,超过60min后,蛋白质浓度上升缓慢.通过实验,采用pH为10的碳酸钠缓冲液,在液固比为80/1(v/w)、超声功率为180W、萃取时间60 min时,浸提5次,可以是蛋白质的得率达到50,69%±1.79%.

  5. Narrowing of the Upwelling Branch of the Brewer-Dobson Circulation and Hadley Cell in Chemistry-Climate Model Simulations of the 21st Century

    Science.gov (United States)

    Li, Feng; Stolarski, Richard S.; Pawson, Steven; Newman, Paul A.; Waugh, Darryn

    2010-01-01

    Changes in the width of the upwelling branch of the Brewer-Dobson circulation and Hadley cell in the 21st Century are investigated using simulations from a coupled chemistry-climate model. In these model simulations the tropical upwelling region narrows in the troposphere and lower stratosphere. The narrowing of the Brewer-Dobson circulation is caused by an equatorward shift of Rossby wave critical latitudes and Eliassen-Palm flux convergence in the subtropical lower stratosphere. In the troposphere, the model projects an expansion of the Hadley cell's poleward boundary, but a narrowing of the Hadley cell's rising branch. Model results suggest that eddy forcing may also play a part in the narrowing of the rising branch of the Hadley cell.

  6. Comparison of ground-based FTIR and Brewer O3 total column with data from two different IASI algorithms and from OMI and GOME-2 satellite instruments

    OpenAIRE

    Blumenstock, T.; J.-M. Flaud; P. Chelin; Eremenko, M.; A. Redondas; Hase, F.; Schneider, M; C. Viatte; Orphal, J

    2011-01-01

    An intercomparison of ozone total column measurements derived from various platforms is presented in this work. Satellite data from Infrared Atmospheric Sounding Interferometer (IASI), Ozone Monitoring Instrument (OMI) and Global Ozone Monitoring Experiment (GOME-2) are compared with data from two ground-based spectrometers (Fourier Transform Infrared spectrometer FTIR and Brewer), located at the Network for Detection of Atmospheric Composition Change (NDACC) super-site of Izaña (Tenerife), m...

  7. Comparison of ground-based FTIR and Brewer O3 total column with data from two different IASI algorithms and from OMI and GOME-2 satellite instruments

    Directory of Open Access Journals (Sweden)

    T. Blumenstock

    2011-03-01

    Full Text Available An intercomparison of ozone total column measurements derived from various platforms is presented in this work. Satellite data from Infrared Atmospheric Sounding Interferometer (IASI, Ozone Monitoring Instrument (OMI and Global Ozone Monitoring Experiment (GOME-2 are compared with data from two ground-based spectrometers (Fourier Transform Infrared spectrometer FTIR and Brewer, located at the Network for Detection of Atmospheric Composition Change (NDACC super-site of Izaña (Tenerife, measured during a campaign from March to June 2009. These ground-based observing systems have already been demonstrated to perform consistent, precise and accurate ozone total column measurements. An excellent agreement between ground-based and OMI/GOME-2 data is observed. Results from two different algorithms for deriving IASI ozone total column are also compared: the European Organisation for the Exploitation of Meteorological Satellites (EUMETSAT/ESA operational algorithm and the LISA (Laboratoire Inter-universitaire des Systèmes Atmosphériques algorithm. A better agreement was found with LISA's analytical approach based on an altitude-dependent Tikhonov-Philips regularization: correlations are 0.94 and 0.89 compared to FTIR and Brewer, respectively; while the operational IASI ozone columns (based on neural network analysis show correlations of 0.90 and 0.85, respectively, compared to the O3 columns obtained from FTIR and Brewer.

  8. A preliminary in vitro assessment of GroBiotic-A, brewer's yeast and fructooligosaccharide as prebiotics for the red drum Sciaenops ocellatus.

    Science.gov (United States)

    Burr, Gary; Hume, Michael; Ricke, Steven; Nisbet, David; Gatlin, Delbert

    2008-01-01

    This study examined the effects of brewers yeast, fructooligosaccharide (FOS), and GroBiotic-A, a mixture of partially autolyzed brewers yeast, dairy components and dried fermentation products, on the intestinal microbial community of red drum, Sciaenops ocellatus. Gastrointestinal (GI) tracts were aseptically removed from three sub-adult red drum previously maintained on a commercial diet and placed in an anaerobic chamber. Intestinal contents were removed, diluted and incubated in vitro in one of four liquid media: normal diet alone, diet + 2% (w/w) GroBiotic-A, diet + 2% brewers yeast, and diet + 2% FOS. After 24 and 48 h of incubation at 25 degrees C, supernatants were removed for volatile fatty acid (VFA) analysis and DNA was extracted for denaturing gradient gel electrophoresis (DGGE) analysis. Polymerase chain reaction (PCR) was performed on a highly conserved region of M 16S rDNA and the amplicons were subjected to DGGE. The microbial community (MC) fingerprint was used to distinguish microbial populations. The intestinal contents incubated with GroBiotic-A had significantly (Pyeast.

  9. Novel trends in electrocatalysis: Extended Brewer hypo-hyper-d-interionic bonding theory and selective interactive grafting of composite bifunctional electrocatalysts for simultaneous anodic hydrogen

    Directory of Open Access Journals (Sweden)

    Neophytides S.G.

    2003-01-01

    Full Text Available Novel Trends in Electrocatalysis: Extended Brewer Hypo-Hyper-d-lnterionic Bonding Theory and Selective Interactive Grafting of Composite Bifunctional Electrocatalysts for Simultaneous Anodic Hydrogen and CO OxidationThe Extended Brewer Interactive Interionic Bonding Theory (EBIIBT has been developed to show the equivalence of interatomic and interionic bonding features, and for their mutual combinations, as well as its effect upon electrocatalytic properties for the hydrogen electrode reactions (HELR. The equivalence of interionic hypo-hyper-d-interelectronic interaction in both metallic and any other ionic state and its effect upon electrocatalytic properties for hydrogen electrode reactions (HELR has been proved and inferred. TG (Thermal Gravimetry analysis of TPR (Temperature Programmed Reduction of mixed hypc-hyper-d-electronic oxides of transition elements was broadly employed to prove the EBIIBT effect as reflected in dramatically decreased individual temperatures of their mutual reduction into intermetallic phases. The same interionic Brewer (and/or intermetallic bonding effect has been confirmed both by UPD of hyper-d-upon hypo-d-electronic substrates and vice versa, and by the shift of bonding peaks in XPS analysis.

  10. Influence of grain type, tallow level, and tallow feeding system on feedlot cattle performance.

    Science.gov (United States)

    Krehbiel, C R; McCoy, R A; Stock, R A; Klopfenstein, T J; Shain, D H; Huffman, R P

    1995-10-01

    Two experiments were conducted to evaluate the effects of grain type, tallow level, and tallow feeding system on finishing steer performance. Experiment 1 involved 256 yearling steers (359 kg) in a 4 x 2 factorial arrangement of treatments. Steers were assigned randomly to one of four tallow feeding systems: 1) 0% tallow fed throughout the experiment; 2) 4% tallow fed throughout the experiment; 3) 0% tallow fed d 1 through 33 and then 4% tallow fed until slaughter; and 4) 4% tallow fed d 1 through 33 and then 0% tallow fed until slaughter. Tallow treatments were applied to diets containing either dry-rolled corn (DRC) of high-moisture corn (HMC). No fat treatment x grain type interaction (P > .10) was observed. Steers fed 4% tallow throughout the experiment, only during d 1 through 33, or only during d 34 until slaughter were more (P tallow. No differences in DMI or ADG were observed (P > .10). In Exp. 2, 120 large-framed steer calves (286 kg) were blocked by weight and allotted randomly within block to one of three treatments consisting of the addition fo 0, 2, or 4% tallow added d 1 and fed for 197 d. Feed efficiency of calves increased linearly (P tallow level. Daily gain was not different (P > .10), but DMI decreased linearly (P tallow level. This research indicates that tallow added during or after grain adaptation to DRC- or HMC-based diets fed to yearling steers will result in similar improvement in feed efficiency, and that including up to 4% tallow to diets fed to large-framed calves can significantly improve feed efficiency.

  11. Fingering phenomena during grain-grain displacement

    Science.gov (United States)

    Mello, Nathália M. P.; Paiva, Humberto A.; Combe, G.; Atman, A. P. F.

    2016-05-01

    Spontaneous formation of fingered patterns during the displacement of dense granular assemblies was experimentally reported few years ago, in a radial Hele-Shaw cell. Here, by means of discrete element simulations, we have recovered the experimental findings and extended the original study to explore the control parameters space. In particular, using assemblies of grains with different geometries (monodisperse, bidisperse, or polydisperse), we measured the macroscopic stress tensor in the samples in order to confirm some conjectures proposed in analogy with Saffman-Taylor viscous fingering phenomena for immiscible fluids. Considering an axial setup which allows to control the discharge of grains and to follow the trajectory and the pressure gradient along the displacing interface, we have applied the Darcy law for laminar flow in fluids in order to measure an "effective viscosity" for each assembly combination, in an attempt to mimic variation of the viscosity ratio between the injected/displaced fluids in the Saffman-Taylor experiment. The results corroborate the analogy with the viscous fluids displacement, with the bidisperse assembly corresponding to the less viscous geometry. But, differently to fluid case, granular fingers only develop for a specific combination of displaced/injected geometries, and we have demonstrated that it is always related with the formation of a force chain network along the finger direction.

  12. Composite circumstellar dust grains

    Science.gov (United States)

    Gupta, Ranjan; Vaidya, Dipak B.; Dutta, Rajeshwari

    2016-10-01

    We calculate the absorption efficiencies of composite silicate grains with inclusions of graphite and silicon carbide in the spectral range 5-25 μm. We study the variation in absorption profiles with volume fractions of inclusions. In particular we study the variation in the wavelength of peak absorption at 10 and 18 μm. We also study the variation of the absorption of porous silicate grains. We use the absorption efficiencies to calculate the infrared flux at various dust temperatures and compare with the observed infrared emission flux from the circumstellar dust around some M-type and asymptotic giant branch stars obtained from IRAS and a few stars from Spitzer satellite. We interpret the observed data in terms of the circumstellar dust grain sizes, shape, composition and dust temperature.

  13. Composite Circumstellar Dust Grains

    CERN Document Server

    Gupta, Ranjan; Dutta, Rajeshwari

    2016-01-01

    We calculate the absorption efficiencies of composite silicate grains with inclusions of graphite and silicon carbide in the spectral range 5--25$\\rm \\mu m$. We study the variation in absorption profiles with volume fractions of inclusions. In particular we study the variation in the wavelength of peak absorption at 10 and 18$\\rm \\mu m$. We also study the variation of the absorption of porous silicate grains. We use the absorption efficiencies to calculate the infrared flux at various dust temperatures and compare with the observed infrared emission flux from the circumstellar dust around some M-Type \\& AGB stars obtained from IRAS and a few stars from Spitzer satellite. We interpret the observed data in terms of the circumstellar dust grain sizes; shape; composition and dust temperature.

  14. THE EFFECT OF ADDING WHOLE WHEAT GRAIN TO FEED MIXTURE ON SLAUGHTER YIELD AND CARCASS COMPOSITION IN GAME PHEASANTS

    Directory of Open Access Journals (Sweden)

    DARIUSZ KOKOSZYŃSKI

    2009-06-01

    Full Text Available The mean body weight of pheasant cocks (1226 g and hens (946.9 g receiving feed mixtures was lower than that of birds fed diets with wheat (♂ 1421.4 g, ♀ 953.2 g. The dressing percentage of both sexes pheasants fed wheat grain also (69.9% was only 0.3% lower than in birds receiving feed mixtures only (70.2%. The carcasses of birds (♂♀ fed the diet with whole wheat grain contained more breast muscles (251.2 g, leg muscles (198.8 g and other carcass components. The carcass percentage of breast muscles, leg muscles, wings and skin with fat was lower, and that of remainders of carcass higher in pheasants receiving wheat grain. In addition, the carcasses of pheasants (♂♀ fed the wheat diets were characterized by a higher weight of meat and fat and lower carcass meat and fat percentage.

  15. Testování Fed modelu

    OpenAIRE

    Hříbalová, Pavlína

    2010-01-01

    Diploma Thesis focuses on Fed Model testing and its credibility on market data. The research is based on Gordon Model and Capital Asset Pricing Model (CAPM), it explains, what the basic features of the Fed Model are and describes its derivation from Gordon Model. The Thesis shows possible Fed Model limitation. It uses the US market, Great Britain and Germany 1979 -- 2011 data to demonstrate validity of the model. Eventually possible reasons of Fed Model development in period 2002 -- 2011 are ...

  16. Preparation and characteristics of beta-glucan concentrate from brewer's yeast as the additive substance in foods

    Directory of Open Access Journals (Sweden)

    Ľubomír Mikuš

    2013-02-01

    Full Text Available Normal 0 21 false false false SK X-NONE X-NONE The brewer¢s yeast was used for preparation of concentrate with content of β-glucan. Hot water extraction (100°C, 5 hours and subsequently an alkaline extraction of sediment using 1 M NaOH at 90°C for 1 hour were used. β-glucan concentrate containing 59,15 % of β-glucan had good functional properties (water binding capacity 13,34 g water/1 g concentrate, fat binding capacity 6,86 g fat/1 g concentrate and indicated biological action too.  At concentration of 2 mg/ml DMSO (dimethylsulfoxid was viability of murine L1210 leukemic cells reduced to 76.15 %. When observing the antioxidant activity it was identified, that the lipid peroxidation in linoleic acid samples was decreased during the presence of β-glucan concentrate. These results and good sensory properties like a bright colour and the pleasant taste and smell indicate, that prepared β-glucan concentrate has a potential to be used to improve the health – beneficial substances in the foods.doi:10.5219/258

  17. 啤酒废酵母自溶条件的研究%Autolysis conditions of waste brewer' s yeast

    Institute of Scientific and Technical Information of China (English)

    杨建梅; 李红; 杜金华

    2012-01-01

    The key factors of waste brewer's yeast autolysis such as temperature, pH value, addition of NaCl and autolysis time were optimized by single factor tests and orthogonal tests in this article. The results indicated that the optimum conditions were obtained as follows: autolysis temperature 50℃,pH value 5.0, addition of NaCl 3% and autolysis time 24h. Under these autolysis conditions, the content of total sugar would reach 2.27g/L, yield of amino-nitragen was 3.98% and yield of the extract was 54.12% in the supernatant fluid.%通过单因素试验和正交试验对影响啤酒废酵母自溶的温度、pH值、NaCl添加量和自溶时间4个关键因素进行了优化,得到了啤酒废酵母自溶最佳工艺条件:自溶温度50℃,自溶pH值为5.0,NaCl添加量为3% (w/w),自溶时间24h,自溶上清液中总糖分含量达到2.27g/L,游离氨基酸态氮得率达到3.98%,抽提物得率达到54.12%.

  18. In search of traceability: two decades of calibrated Brewer UV measurements in Sodankylä and Jokioinen

    Science.gov (United States)

    Heikkilä, Anu; Sakari Mäkelä, Jakke; Lakkala, Kaisa; Meinander, Outi; Kaurola, Jussi; Koskela, Tapani; Matti Karhu, Juha; Karppinen, Tomi; Kyrö, Esko; de Leeuw, Gerrit

    2016-11-01

    The two Brewer spectrophotometers of the Finnish Meteorological Institute at Jokioinen and Sodankylä have been operated according to the highest levels of the WMO/GAW (World Meteorological Organization/Global Atmosphere Watch) recommendations with rigorous quality control and quality assurance. The calibration of the instruments is based on annual recalibrations of primary standard lamps in the VTT MIKES Metrology National Standards Laboratory in Finland and an exhaustive measurement program with measurements of standard and working lamps in the on-site optical laboratories. Over the years, the maintenance of the calibration has produced data sets of approximately 2000 lamp scans for both instruments. An extensive re-examination of the lamp measurements and the response of the spectrophotometers was carried out. The primary standard lamps were found to age on an average rate of 0.3 % per burn. The responsivity at wavelength 311 nm was found to exhibit both long-term and short-term changes. The overall long-term change was declining. In addition, abrupt changes of as large as 25 % were detected. The short-term changes were found to fluctuate on time frames shorter than the interval between the measurements of the primary standard lamps. This underlines the importance of the use of more frequently measured working standard lamps.

  19. Whole Grains and Fiber

    Science.gov (United States)

    ... whole grains. Does not contain partially hydrogenated oils. Dietary Fiber Dietary fiber is the term for several materials that make ... water. When eaten regularly as part of a diet low in saturated fat and trans fat soluble fiber has been associated with increased diet quality and ...

  20. Formation on grain surfaces

    NARCIS (Netherlands)

    Cazaux, S; Tielens, AGGM

    2004-01-01

    The most abundant interstellar molecule, H-2, is generally thought to form by recombination of H atoms on grain surfaces. On surfaces, hydrogen atoms can be physisorbed and chemisorbed and their mobility can be governed by quantum mechanical tunneling or thermal hopping. We have developed a model fo

  1. Evaluation of distiller’s dried grains with solubles (DDGS) from different grain sources as dietary protein for hybrid tilapia, Oreochromis niloticus x O. Aureus

    Science.gov (United States)

    The effects of distiller’s dried grains with solubles (DDGS) from different sources on growth performance, hematology, and immunity of hybrid tilapia, Oreochromis niloticus x O. aureus, were evaluated. Sex-reversed, all-male hybrid tilapia (3.72 ± 0.08 g initial weight) were fed diets in which 30% o...

  2. Grain Boundary Segregation in Metals

    CERN Document Server

    Lejcek, Pavel

    2010-01-01

    Grain boundaries are important structural components of polycrystalline materials used in the vast majority of technical applications. Because grain boundaries form a continuous network throughout such materials, their properties may limit their practical use. One of the serious phenomena which evoke these limitations is the grain boundary segregation of impurities. It results in the loss of grain boundary cohesion and consequently, in brittle fracture of the materials. The current book deals with fundamentals of grain boundary segregation in metallic materials and its relationship to the grain boundary structure, classification and other materials properties.

  3. Why Is It Important to Eat Grains, Especially Whole Grains?

    Science.gov (United States)

    ... Nutrients and health benefits Print Share Why is it important to eat grains, especially whole grains? Eating ... diabetes. Fiber is important for proper bowel function. It helps reduce constipation and diverticulosis. Fiber-containing foods ...

  4. Effect of distillers grains or corn supplementation frequency on forage intake and digestibility.

    Science.gov (United States)

    Loy, T W; MacDonald, J C; Klopfenstein, T J; Erickson, G E

    2007-10-01

    Ten ruminally cannulated heifers (BW = 416 kg; SD = 24) were used to test the effect of the form and frequency of supplemental energy on forage DMI and digestibility. Five treatments were arranged in a replicated, 5 x 4 Latin rectangle (n = 8), and included no supplement (control), dry-rolled corn (DRC) fed daily, DRC fed on alternate days (DRC-A), dried distillers grains plus solubles (DDGS) fed daily, and DDGS fed on alternate days (DDGS-A). Supplements fed daily were fed at 0.40% of BW, whereas alternate day-fed supplements were fed at 0.80% of BW every other day. Chopped grass hay (8.2% CP) was fed to allow ad libitum DMI, and the intake pattern was measured. Control heifers had greater (P meals and spent less (P time eating than those supplemented daily. Average rumen pH was greater (P = 0.05) for control than supplemented heifers (6.30 vs. 6.19). Control heifers had greater (P = 0.04) rates and extents of NDF disappearance than supplemented heifers. Rate of hay NDF disappearance was lower (P = 0.02) for DRC than for DDGS. Supplementation decreased hay DMI and changed digestion kinetics. Supplementation frequency affected amount and pattern of DMI. Rate of hay NDF disappearance was greater for DDGS than DRC.

  5. Consumo, digestibilidade dos nutrientes, produção e composição do leite de vacas alimentadas com resíduo de cervejaria fermentado - DOI: 10.4025/actascianimsci.v32i1.6990 Nutrient intake and digestibility and milk yield of cows fed with fermented brewer’s grain - DOI: 10.4025/actascianimsci.v32i1.6990

    Directory of Open Access Journals (Sweden)

    Eder Bublitz

    2009-12-01

    Full Text Available Objetivou-se avaliar a inclusão de 0, 5, 10 e 15% do resíduo de cervejaria fermentado (RCF nas rações de vacas leiteiras sobre o consumo e coeficiente de digestibilidade total (CD dos nutrientes, produção e qualidade do leite. Foram utilizadas 28 vacas da raça Holandesa, com aproximadamente 101 ± 21 dias de lactação, e peso corporal médio de 612 ± 110 kg, alimentadas com silagem de milho, silagem pré-secada de azevém, RCF e concentrado. Foi utilizado o delineamento inteiramente casualizado. Os dados de consumo e CD dos nutrientes, produção e qualidade do leite foram submetidos à análise de regressão considerando 5% de probabilidade. O consumo de matéria seca (MS dos animais foi “ad libitum”. Os teores de RCF nas rações não alteraram (p > 0,05 o consumo dos nutrientes. Os CD da matéria orgânica (MO, carboidratos totais (CHT, fibra em detergente neutro (FDN e fibra em detergente ácido (FDA não foram influenciados (p > 0,05 pelos teores de RCF, contudo, verificou-se efeito linear positivo com relação aos CD da MS, proteína bruta (PB e extrato etéreo (EE em função da inclusão do RCF nas rações. A inclusão do RCF nas rações não influenciou (p > 0,05 na produção e qualidade do leite. Conclui-se que o RCF pode ser incluído até 15% nas rações de vacas leiteiras sem alterar o consumo de nutrientes e a produção e qualidade do leiteThe objective this work was to evaluate the inclusion of 0, 5, 10 and 15% of the fermented brewer’s grain (FBG in diets of dairy cows on intake and total digestibility coefficient (DC of nutrients, production and milk quality. Twenty-eight Holstein cows were used, with 100 ± 21 days in milk, and average body weight (BW of 612 ± 110 kg, fed with corn silage, ryegrass silage, FBG and concentrate. Intake data and nutrient’s DC, production and milk quality were subjected to regression analysis to 5% of probability. The DM intake of animals was ad libitum. The FBG inclusion

  6. Características da carcaça e da carne de cordeiros terminados com dietas contendo diferentes proporções de silagens de grãos de milho - DOI: 10.4025/actascianimsci.v31i2.3451 Components of live weight, carcass and meat caracteristics of lambs fed with diets containing corn grain silage - DOI: 10.4025/actascianimsci.v31i2.3451

    Directory of Open Access Journals (Sweden)

    Eliane Gasparino

    2009-08-01

    Full Text Available Foram avaliados componentes do peso vivo, características objetivas e subjetivas da carcaça, características físico-químicas do músculo Longissimus lumborum e a proporção dos principais tecidos que compõem o lombo de 24 cordeiros, machos não castrados, mestiços Hampshire Down, terminados em confinamento e alimentados com dietas diferentes à base de silagem de grãos de milho úmidos, com inclusão de girassol ou ureia. Os tratamentos não afetaram: peso vivo ao abate, peso da carcaça quente, rendimentos de carcaça, sangue, pele, aparelho reprodutor com bexiga, aparelho respiratório, baço, rins e gordura perirrenal, fígado, coração, trato gastrintestinal vazio, cabeça e patas. Não houve diferença significativa para peso da carcaça fria, condição corporal, conformação de carcaça, cobertura de gordura, consistência de gordura, cor da carne, espessura de gordura, área de olho de lombo, proteína bruta; proporções de: músculo, ossos, gordura e outros tecidos do músculo Longissimus lumborum. Perda por cozimento, índice de fragmentação miofibrilar e índices de cores L*, a* e b* não diferiram quanto aos tratamentos. Os cordeiros podem ser terminados com qualquer uma das dietas, pois apresentaram semelhanças para as características analisadasThe study evaluated the live weight components, objective and subjective carcass characteristics and physical-chemical characteristics of the Longissimus lumborum muscle and the proportion of tissues that constitute the loin of twenty-four intact males lambs, Hampshire Down crossbreed, fattened in a drylot system. The lambs were fed with different diets based on corn grain silage with the addition of sunflower seeds or urea. The treatments did not affect live weight and hot carcass weight at slaughter, carcass yield, blood, skin, reproductive system with bladder, respiratory system, spleen, kidneys and perineal fat, liver, heart, empty gastrointestinal tract, head or feet

  7. Grain rotation mediated by grain boundary dislocations in nanocrystalline platinum

    Science.gov (United States)

    Wang, Lihua; Teng, Jiao; Liu, Pan; Hirata, Akihiko; Ma, En; Zhang, Ze; Chen, Mingwei; Han, Xiaodong

    2014-01-01

    Grain rotation is a well-known phenomenon during high (homologous) temperature deformation and recrystallization of polycrystalline materials. In recent years, grain rotation has also been proposed as a plasticity mechanism at low temperatures (for example, room temperature for metals), especially for nanocrystalline grains with diameter d less than ~15 nm. Here, in tensile-loaded Pt thin films under a high-resolution transmission electron microscope, we show that the plasticity mechanism transitions from cross-grain dislocation glide in larger grains (d>6 nm) to a mode of coordinated rotation of multiple grains for grains with d<6 nm. The mechanism underlying the grain rotation is dislocation climb at the grain boundary, rather than grain boundary sliding or diffusional creep. Our atomic-scale images demonstrate directly that the evolution of the misorientation angle between neighbouring grains can be quantitatively accounted for by the change of the Frank–Bilby dislocation content in the grain boundary. PMID:25030380

  8. Substituição do milho por polpa cítrica em rações com alta proporção de concentrado para cordeiros confinados Replacement of corn by citrus pulp in high grain diets fed to feedlot lambs

    Directory of Open Access Journals (Sweden)

    Gustavo Henrique Rodrigues

    2008-06-01

    Full Text Available A polpa cítrica desidratada (PC é um subproduto altamente energético e com potencial para substituir o milho em rações de cordeiros desmamados precocemente. Dois experimentos foram realizados para avaliarem-se os efeitos da substituição do milho por PC no desempenho de cordeiros e na digestibilidade das rações. No Experimento 1, 64 cordeiros Santa Inês, com peso vivo inicial de 18 (±0,6 kg e 73 (±1 dias de idade, foram utilizados para avaliar o ganho de peso médio diário (GMD, o consumo de matéria seca (CMS e a conversão alimentar (CA. No experimento 2, 12 cordeiros foram mantidos em gaiolas para ensaio de metabolismo para determinar a digestibilidade aparente das rações no trato digestório total e o metabolismo de nitrogênio. Nos dois experimentos, os cordeiros foram alimentados com uma ração contendo 90% de concentrado (milho moído e/ou PC, farelo de soja e minerais e 10% de feno de "coastcross" (Cynodon spp. A PC foi adicionada em 23,7; 46,1 e 68,4% da MS, substituindo o milho em 33, 67 e 100%, respectivamente. No Experimento 1, foi observado a maximização do GMD (267g dia-1 e do CMS (1,01kg dia-1, até o teor de substituição de 33% do milho pela PC. No Experimento 2, houve efeito linear (P0,05 entre os tratamentos na digestiblidade aparente da matéria orgânica e no balanço de nitrogênio.Dried citrus pulp (DCP is a high energy byproduct and may be used to replace corn in early weaned lamb diets. Two trials were performed to evaluate the effects of replacing corn by dried citrus pulp on lamb performance and diet digestibility. In trial 1, 64 Santa Ines ram lambs (initial BW 18 ± 0.6kg and 73 ± 1 days old were used to evaluate average daily gain (ADG, dry matter intake (DMI and feed conversion (FC. In trial 2, 12 ram lambs were placed in metabolism crates to evaluate N metabolism and apparent digestibility of diets. In both trials, lambs were fed a 90% concentrate (ground corn and/or DCP, soybean meal and

  9. Predictive coarse-graining

    Science.gov (United States)

    Schöberl, Markus; Zabaras, Nicholas; Koutsourelakis, Phaedon-Stelios

    2017-03-01

    We propose a data-driven, coarse-graining formulation in the context of equilibrium statistical mechanics. In contrast to existing techniques which are based on a fine-to-coarse map, we adopt the opposite strategy by prescribing a probabilistic coarse-to-fine map. This corresponds to a directed probabilistic model where the coarse variables play the role of latent generators of the fine scale (all-atom) data. From an information-theoretic perspective, the framework proposed provides an improvement upon the relative entropy method [1] and is capable of quantifying the uncertainty due to the information loss that unavoidably takes place during the coarse-graining process. Furthermore, it can be readily extended to a fully Bayesian model where various sources of uncertainties are reflected in the posterior of the model parameters. The latter can be used to produce not only point estimates of fine-scale reconstructions or macroscopic observables, but more importantly, predictive posterior distributions on these quantities. Predictive posterior distributions reflect the confidence of the model as a function of the amount of data and the level of coarse-graining. The issues of model complexity and model selection are seamlessly addressed by employing a hierarchical prior that favors the discovery of sparse solutions, revealing the most prominent features in the coarse-grained model. A flexible and parallelizable Monte Carlo - Expectation-Maximization (MC-EM) scheme is proposed for carrying out inference and learning tasks. A comparative assessment of the proposed methodology is presented for a lattice spin system and the SPC/E water model.

  10. Grain alcohol study: summary

    Energy Technology Data Exchange (ETDEWEB)

    The study has concentrated upon a detailed examination of all considerations involved in the production, use, and marketing of ethyl alcohol (Ethanol) as produced from the fermentation of agricultural grains. Each parameter was examined in the light of current energy markets and trends; new sources and technological, and processes for fermentation, the capability of the agricultural industry to support fermentaton demand; the optimization of value of agricultureal crops; and the efficiencies of combining related industries. Anhydrous (200 proof) ethanol makes an excellent blending component for all present automotive fuels and an excellent octane additive for unleaded fuels in proportions up to 35% without requiring modifications to current engines. There is no difference between ethanol produced by fermentation and ethanol produced synthetically from petroleum. The decision to produce ethanol one way or the other is purely economic. The agricultural industry can support a major expansion in the fermentation industry. The residue (distillers grains) from the fermentation of corn for ethanol is an excellent and economical feed for livestock and poultry. A reliable supply of distillers grains can assist in making the large beef feedlot operations more economically viable. The source materials, fuels, products and by-products of an ethanol plant, beef feedlot, gas biodigester plant, municipal waste recovery plant and a steam generated electrical plant are interrelated and mutually beneficial for energy efficiencies and economic gains when co-located. The study concludes that the establishment of such agricultural-environment industrial energy complexes, would provide a broad range of significant benefits to Indiana.

  11. Grain alcohol study: summary

    Energy Technology Data Exchange (ETDEWEB)

    The study has concentrated upon a detailed examination of all considerations involved in the production, use, and marketing of ethyl alcohol (ethanol) as produced from the fermentation of agricultural grains. Each parameter was examined in the light of current energy markets and trends; new sources and technological, and processes for fermentation, the capability of the agricultural industry to support fermentation demand; the optimizaton of value of agricultural crops; and the efficiencies of combining related industries. Ahydrous (200 proof) ethanol makes an excellent blending component for all present automotive fuels and an excellent octane additive for unleaded fuels in proportions up to 35% without requiring modifications to current engines. There is no difference between ethanol produced by fermentation and ethanol produced synthetically from petroleum. The decision to produce ethanol one way or the other is purely economic. The agricultural industry can support a major expansion in the fermentation industry. The residue (distillers grains) from the fermentation of corn for ethanol is an excellent and economical feed for livestock and poultry. A reliable supply of distillers grain can assist in making the large beef feedlot operations more economically viable. The source materials, fuels, products and by-products of an ethanol plant, beef feedlot, gas biodigester plant, municipal waste recovery plant and a steam generated electrical plant are interrelated and mutually beneficial for energy efficiencies and economic gains when co-located. The study concludes that the establishment of such agricultural- environment industrial energy complexes, would provide a broad range of significant benefits to Indiana.

  12. Direct-Sun column ozone retrieval by the ultraviolet multifilter rotating shadow-band radiometer and comparison with those from Brewer and Dobson spectrophotometers.

    Science.gov (United States)

    Gao, W; Slusser, J; Gibson, J; Scott, G; Bigelow, D; Kerr, J; McArthur, B

    2001-07-01

    A methodology for direct-Sun ozone retrieval using the ultraviolet multifilter rotating shadow-band radiometer (UV-MFRSR) is presented. Total vertical column ozone was retrieved in three stations: Mauna Loa, Hawaii, in the U.S., and Regina, Saskatchewan, and Toronto, Ontario, in Canada, from direct solar irradiances of the UV-MFRSR at 325-, 305-, 332-, and 311-nm channels (2-nm FWHM). The total uncertainty of ozone retrievals in this study is +/-2.0%. For Mauna Loa the mean ratios of the UV-MFRSR column ozone retrievals to the collocated Dobson and Brewer were 0.998 and 0.986 between May and September of 1999. The mean ratio of UV-MFRSR retrievals to the collocated Brewer retrievals was 1.012 in Toronto between April and August of 1999, and the mean ratio of retrievals of the UV-MFRSR to the collocated Brewer was 0.988 in Regina between June and September of 1999. Total vertical column ozone values for solar zenith angles of >70 degrees were not considered, because of the signal-to-noise ratio and the angular response of the instruments, and were not used in the evaluation. The advantages of total vertical column ozone retrieval using UV-MFRSR include relatively low cost, computer-controlled operation, automated calibration stability checks, and minimal maintenance. It allows for the real-time measurement of total vertical column ozone. The UV-MFRSR is being used at 28 sites across the United States and 2 sites in Canada that form the U.S. Department of Agriculture UV-B Radiation Monitoring and Research Program. This constitutes a unique network of total vertical colunm ozone measurement.

  13. High grain diets perturb rumen and plasma metabolites and induce inflammatory responses in early lactation dairy cows

    Directory of Open Access Journals (Sweden)

    B. N. Ametaj

    2010-04-01

    Full Text Available Immediately after parturition dairy cows are fed diets containing high proportions of grain which are associated with high incidence of metabolic disorders. However, the reason behind these effects is not clear. The goal of this study was to investigate metabolic and immune responses of early postpartum dairy cows to feeding increasing proportions of barley grain in the diet. Rumen endotoxin content increased several fold and rumen fluid pH was lower in cows fed the highest amount of barley grain. Results indicate that feeding high proportions of barley grain in early lactation dairy cows was associated with induction of an acute phase response and changes in multiple plasma metabolites. Further research is warranted to understand the mechanism(s by which feeding of barley grain causes disturbances in plasma metabolites and stimulates an inflammatory response in dairy cows.

  14. FED baseline engineering studies report

    Energy Technology Data Exchange (ETDEWEB)

    Sager, P.H.

    1983-04-01

    Studies were carried out on the FED Baseline to improve design definition, establish feasibility, and reduce cost. Emphasis was placed on cost reduction, but significant feasibility concerns existed in several areas, and better design definition was required to establish feasibility and provide a better basis for cost estimates. Design definition and feasibility studies included the development of a labyrinth shield ring concept to prevent radiation streaming between the torus spool and the TF coil cryostat. The labyrinth shield concept which was developed reduced radiation streaming sufficiently to permit contact maintenance of the inboard EF coils. Various concepts of preventing arcing between adjacent shield sectors were also explored. It was concluded that installation of copper straps with molybdenum thermal radiation shields would provide the most reliable means of preventing arcing. Other design studies included torus spool electrical/structural concepts, test module shielding, torus seismic response, poloidal conditions in the magnets, disruption characteristics, and eddy current effects. These additional studies had no significant impact on cost but did confirm the feasibility of the basic FED Baseline concept.

  15. Over-expression of GSH1 gene and disruption of PEP4 gene in self-cloning industrial brewer's yeast.

    Science.gov (United States)

    Wang, Zhao-Yue; He, Xiu-Ping; Zhang, Bo-Run

    2007-11-01

    Foam stability is often influenced by proteinase A, and flavor stability is often affected by oxidation during beer storage. In this study, PEP4, the gene coding for proteinase A, was disrupted in industrial brewing yeast. In the meantime, one copy of GSH1 gene increased in the same strain. GSH1 is responsible for gamma-glutamylcysteine synthetase, a rate-limiting enzyme for synthesis of glutathione which is one kind of important antioxidant and beneficial to beer flavor stability. In order to improve the brewer's yeast, plasmid pYPEP, pPC and pPCG1 were firstly constructed, which were recombined plasmids with PEP4 gene, PEP4's disruption and PEP4's disruption+GSH1 gene respectively. These plasmids were verified to be correct by restriction enzymes' assay. By digesting pPCG1 with AatII and PstI, the DNA fragment for homologous recombination was obtained carrying PEP4 sequence in the flank and GSH1 gene internal to the fragment. Since self-cloning technique was applied in the study and the modified genes were from industrial brewing yeast itself, the improved strains, self-cloning strains, were safe to public. The genetic stability of the improved strains was 100%. The results of PCR analysis of genome DNA showed that coding sequence of PEP4 gene had been deleted and GSH1 gene had been inserted into the locus of PEP4 gene in self-cloning strains. The fermentation ability of self-cloning strain, SZ-1, was similar to that of the host. Proteinase A could not be detected in beer brewed with SZ-1, and GSH content in the beer increased 35% compared to that of the host, Z-1.

  16. Beneficial Effect of Brewers' Yeast Extract on Daily Activity in a Murine Model of Chronic Fatigue Syndrome

    Directory of Open Access Journals (Sweden)

    Takashi Takahashi

    2006-01-01

    Full Text Available The aim of this study was to assess the effect of Brewers' yeast extract (BYE on daily activity in a mouse model of chronic fatigue syndrome (CFS. CFS was induced by repeated injection of Brucella abortus (BA antigen every 2 weeks. BYE was orally administered to mice in a dose of 2 g per kg per day for 2 weeks before injecting BA and for 4 weeks thereafter. We evaluated daily running activity in mice receiving BYE as compared with that in untreated mice. Weekly variation of body weight (BW and survival in both groups was monitored during the observation period. Spleen weight (SW, SW/BW ratio, percent splenic follicular area and expression levels of interferon-γ (IFN-γ and interleukin-10 (IL-10 mRNA in spleen were determined in both groups at the time of sacrifice. The daily activity during 2 weeks after the second BA injection was significantly higher in the treated group than in the control. There was no difference in BW between both groups through the experimental course. Two mice in the control died 2 and 7 days after the second injection, whereas no mice in the treated group died. Significantly decreased SW and SW/BW ratio were observed in the treated mice together with elevation of splenic follicular area. There were suppressed IFN-γ and IL-10 mRNA levels in spleens from the treated mice. Our results suggest that BYE might have a protective effect on the marked reduction in activity following repeated BA injection via normalization of host immune responses.

  17. Beneficial effect of brewers' yeast extract on daily activity in a murine model of chronic fatigue syndrome.

    Science.gov (United States)

    Takahashi, Takashi; Yu, Fei; Zhu, Shi-Jie; Moriya, Junji; Sumino, Hiroyuki; Morimoto, Shigeto; Yamaguchi, Nobuo; Kanda, Tsugiyasu

    2006-03-01

    The aim of this study was to assess the effect of Brewers' yeast extract (BYE) on daily activity in a mouse model of chronic fatigue syndrome (CFS). CFS was induced by repeated injection of Brucella abortus (BA) antigen every 2 weeks. BYE was orally administered to mice in a dose of 2 g per kg per day for 2 weeks before injecting BA and for 4 weeks thereafter. We evaluated daily running activity in mice receiving BYE as compared with that in untreated mice. Weekly variation of body weight (BW) and survival in both groups was monitored during the observation period. Spleen weight (SW), SW/BW ratio, percent splenic follicular area and expression levels of interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) mRNA in spleen were determined in both groups at the time of sacrifice. The daily activity during 2 weeks after the second BA injection was significantly higher in the treated group than in the control. There was no difference in BW between both groups through the experimental course. Two mice in the control died 2 and 7 days after the second injection, whereas no mice in the treated group died. Significantly decreased SW and SW/BW ratio were observed in the treated mice together with elevation of splenic follicular area. There were suppressed IFN-gamma and IL-10 mRNA levels in spleens from the treated mice. Our results suggest that BYE might have a protective effect on the marked reduction in activity following repeated BA injection via normalization of host immune responses.

  18. Rumen microbial changes in cattle fed diets with or without salinomycin.

    OpenAIRE

    Olumeyan, D B; Nagaraja, T. G.; Miller, G.W; Frey, R A; Boyer, J E

    1986-01-01

    Four rumen-fistulated steers, randomly assigned to two groups (control and salinomycin fed) were used to monitor the changes in rumen microbial populations and volatile fatty acids (VFA) concentrations associated with feeding salinomycin (0.22 mg X kg-1 X day-1). Steers were adapted to an alfalfa hay and grain (80:20) diet before supplementing the diet with salinomycin, and then the diet was changed to 50:50 and 20:80 ratios of alfalfa hay to grain at 2-week intervals. Rumen samples for total...

  19. Desempenho e digestibilidade de nutrientes em frangos de corte alternativos alimentados com silagem de grãos úmidos de sorgo - DOI: 10.4025/actascianimsci.v29i3.557 Performance and nutrient digestibility in alternative broilers fed with high-moisture sorghum grains silage - DOI: 10.4025/actascianimsci.v29i3.557

    Directory of Open Access Journals (Sweden)

    Vanessa Cristina Pelícia

    2007-12-01

    Full Text Available O objetivo do trabalho foi avaliar os níveis de inclusão de silagem de grãos úmidos de sorgo (SGUS, em substituição ao milho seco da ração sobre desempenho, rendimento de carcaça, cortes e gordura abdominal, além de avaliação econômica da criação alternativa, no período de 1 a 49 dias de idade. Também foi avaliada a digestibilidade das rações aos 21 dias de idade. Para as análises de desempenho e rendimento de carcaça, foram distribuídos, em galpão, 600 pintos machos (Cobb, um dia de idade, em delineamento em blocos casualizados, com seis tratamentos (0, 10, 20, 30, 40 e 50% de inclusão SGUS, em substituição ao milho seco da ração, e quatro repetições de 25 aves cada. Para digestibilidade, foram alojados em câmara termoneutra, 72 pintos (Cobb, em 24 gaiolas, sendo três aves/gaiola, 12 aves/tratamento. Os tratamentos experimentais foram os mesmos adotados para o galpão. A SGUS pode substituir em até 50% o milho seco das rações de frangos alternativos, criados até 49 dias de idade, sem alterar desempenho, rendimento de carcaça, cortes e gordura abdominal, bem como a digestibilidade de nutrientes aos 21 dias de idade. Entretanto, o maior retorno econômico e melhor fator de produção foram obtidos com 10% de substituição.The objective of this study was to evaluate levels of inclusion of high-moisture sorghum grains silage (HMSS in replacement of dry corn in diet on performance, carcass yield, cuts and abdominal fat, and also economic analysis of alternative system at 1 to 49 d of age. The digestibility of the diets was determined at 21 d of age. For performance and carcass yield analysis, 600 one-day-old male broiler chicks (Cobb were used, installed in a broiler house, in a randomized blocks, with six treatments based on the inclusion of 0, 10, 20, 30, 40 and 50% of HMSS in replacement of dry corn, and four replicates/treatment with 25 chicks each. For digestibility, 72 one-dayold male chicks (Cobb were

  20. Reduced use of antibiotic growth promoters in diets fed to weanling pigs: dietary tools, part 2.

    Science.gov (United States)

    Stein, Hans H; Kil, Dong Y

    2006-01-01

    Diets formulated to maximize performance of weanling pigs need to support the development of intestinal tissue, support intestinal colonization with beneficial, mainly lactic acid-producing bacteria, and support development of the intestinal and overall immune system. This objective is not likely to be achieved using one single strategy, but there is strong evidence that diets formulated with cereal grains other than corn, with a low concentration of crude protein and with the use of direct-fed microbials, will improve intestinal health and performance of weanling pigs. Further improvements may be observed if the grain part of the diet is fermented prior to feeding or if the diet is fed in a liquid form, but the need for specialized equipment limit the implementation of this strategy. Dietary supplements such as essential oils and nucleosides or nucleotides may also be useful, but more research is needed to verify the effects of these substances.

  1. Microbial production of volatile sulphur compounds in the large intestine of pigs fed two different diets

    DEFF Research Database (Denmark)

    Poulsen, Henrik Vestergaard; Jensen, Bent Borg; Finster, Kai

    2012-01-01

    Aims: To investigate the production of volatile sulphur compounds (VSC) in segments of the large intestine of pigs and to assess the impact of diet on this production. Methods and Results: Pigs were fed two diets based on either wheat and barley (STD) or wheat and dried distillers grains with sol......Aims: To investigate the production of volatile sulphur compounds (VSC) in segments of the large intestine of pigs and to assess the impact of diet on this production. Methods and Results: Pigs were fed two diets based on either wheat and barley (STD) or wheat and dried distillers grains...... significantly higher in the STD group. Conversely, the net methanethiol production rate was significantly higher in the DDGS-group, while no difference was observed for dimethyl sulphide. The number of sulphate reducing bacteria and total bacteria were determined by quantitative PCR and showed a significant...

  2. Grain Destruction in Interstellar Shocks

    OpenAIRE

    1995-01-01

    Interstellar shock waves can erode and destroy grains present in the shocked gas, primarily as the result of sputtering and grain-grain collisions. Uncertainties in current estimates of sputtering yields are reviewed. Results are presented for the simple case of sputtering of fast grains being stopped in cold gas. An upper limit is derived for sputtering of refractory grains in C-type MHD shocks: shock speeds $v_s \\gtrsim 50 \\kms$ are required for return of more than 30\\% of the silicate to t...

  3. Whole grains and human health.

    Science.gov (United States)

    Slavin, Joanne

    2004-06-01

    Epidemiological studies find that whole-grain intake is protective against cancer, CVD, diabetes, and obesity. Despite recommendations to consume three servings of whole grains daily, usual intake in Western countries is only about one serving/d. Whole grains are rich in nutrients and phytochemicals with known health benefits. Whole grains have high concentrations of dietary fibre, resistant starch, and oligosaccharides. Whole grains are rich in antioxidants including trace minerals and phenolic compounds and these compounds have been linked to disease prevention. Other protective compounds in whole grains include phytate, phyto-oestrogens such as lignan, plant stanols and sterols, and vitamins and minerals. Published whole-grain feeding studies report improvements in biomarkers with whole-grain consumption, such as weight loss, blood-lipid improvement, and antioxidant protection. Although it is difficult to separate the protective properties of whole grains from dietary fibre and other components, the disease protection seen from whole grains in prospective epidemiological studies far exceeds the protection from isolated nutrients and phytochemicals in whole grains.

  4. The effect of grain type and processing on chewing activity in horses

    DEFF Research Database (Denmark)

    Brøkner, Christine; Nørgaard, Peder; Eriksen, Lis;

    2005-01-01

    The aims of the present experiment were to study the effect of grain type and processing on chewing activity in horses. Three adult trotters (Exp.I) and 3 adult Icelandic horses (Exp.II) were fed 3 daily meals during 3 consecutive days in two 3 x 3 completely randomized block design experiments. ...

  5. Crop rotation affects corn, grain sorghum, and soybean yields and nitrogen recovery

    Science.gov (United States)

    Long-term cropping system and fertilizer N studies are essential towards understanding production potential and yield stability of corn (Zea mays L.), grain sorghum [Sorghum bicolor (L.) Moench], and soybean [Glycine max (L.) Merr.] in rain-fed environments. A no-till experiment (2007-13) was conduc...

  6. Alternative grains in nutrition

    Directory of Open Access Journals (Sweden)

    Jevcsák Sz.

    2016-12-01

    Full Text Available Many people suffer from gluten sensitivity or gluten intolerance. They have to avoid or limit their gluten intake. Sorghum and millet are gluten-free cereals, wherefore persons with gluten sensitivity or gluten intolerance could consume them. Moreover, they have a lot of positive effects due to their phenolic compounds as phenol acid or flavonoid. Antioxidant activity in sorghum is especially high in comparison with other cereals. Our aim was to compare literature data about the chemical compositions of sorghum and millet with other grains.

  7. Measurements of the total ozone column using a Brewer spectrophotometer and TOMS and OMI satellite instruments over the Southern Space Observatory in Brazil

    Science.gov (United States)

    Vaz Peres, Lucas; Bencherif, Hassan; Mbatha, Nkanyiso; Passaglia Schuch, André; Toihir, Abdoulwahab Mohamed; Bègue, Nelson; Portafaix, Thierry; Anabor, Vagner; Kirsch Pinheiro, Damaris; Paes Leme, Neusa Maria; Valentin Bageston, José; Schuch, Nelson Jorge

    2017-01-01

    This paper presents 23 years (1992-2014) of quasi-continuous measurements of the total ozone column (TOC) over the Southern Space Observatory (SSO) in São Martinho da Serra, Brazil (29.26° S, 53.48° and 488 m altitude). The TOC was measured by a Brewer spectrometer, and the results are also compared to daily and monthly observations from the TOMS (Total Ozone Mapping Spectrometer) and OMI (Ozone Monitoring Instrument) satellite instruments. Analyses of the main interannual modes of variability computed using the wavelet transform method were performed. A favorable agreement between the Brewer spectrophotometer and satellite datasets was found. The seasonal TOC variation is dominated by an annual cycle, with a minimum of approximately 260 DU in April and a maximum of approximately 295 DU in September. The wavelet analysis applied in the SSO TOC anomaly time series revealed that the Quasi-Biennial Oscillation (QBO) modulation was the main mode of interannual variability. The comparison between the SSO TOC anomaly time series with the QBO index revealed that the two are in opposite phases.

  8. Antiproliferation and induction of cell death of Phaffia rhodozyma (Xanthophyllomyces dendrorhous) extract fermented by brewer malt waste on breast cancer cells.

    Science.gov (United States)

    Teo, Ivy Tuang Ngo; Chui, Chung Hin; Tang, Johnny Cheuk On; Lau, Fung Yi; Cheng, Gregory Yin Ming; Wong, Raymond Siu Ming; Kok, Stanton Hon Lung; Cheng, Chor Hing; Chan, Albert Sun Chi; Ho, Kwok Ping

    2005-11-01

    Astaxanthin has been shown to have antiproliferative activity on breast cancer and skin cancer cells. However, the high cost of production, isolation and purification of purified astaxanthin from natural sources or chemically synthetic methods limit its usage on cancer therapy. We show that astaxanthin could be produced by fermentating the Phaffia rhodozyma (Xanthophyllomyces dendrorhous) yeast cells with brewer malt waste using a 20 L B. Braun fermentor. The percentage composition of astaxanthin from the P. rhodozyma was >70% of total pigment as estimated by the high performance liquid chromatographic analysis. Furthermore, the antiproliferative activity of this P. rhodozyma cell extract (PRE) was demonstrated on breast cancer cell lines including the MCF-7 (estrogen receptor positive) and MDA-MB231 (estrogen receptor negative) by using the [3-(4,5-dimethylthiazol-2-yl)-5-(3-arboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium] (MTS) assay. No apoptotic cell death, but growth inhibitory effect was induced after 48 h of PRE incubation as suggested by morphological investigation. Anchorage-dependent clonogenicity assay showed that PRE could reduce the colony formation potential of both breast cancer cell lines. Cell death was observed from both breast cancer cell lines after incubation with PRE for 6 days. Taken together, our results showed that by using an economic method of brewer malt waste fermentation, we obtained P. rhodozyma with a high yield of astaxanthin and the corresponding PRE could have short-term growth inhibition and long-term cell death activity on breast cancer cells.

  9. The use of whole grain sorghum in broiler feeds

    Directory of Open Access Journals (Sweden)

    EA Fernandes

    2013-09-01

    Full Text Available In broiler feed manufacturing, grinding may significantly increase costs due to electric energy use. This study evaluated the performance and the morphometrics of the gastrointestinal tract of broilers. According to a completely randomized experimental design, 2,400 birds (1,200 males and 1,200 females were submitted to five treatments of eight replicates each. Treatments consisted of diets based corn or sorghum as follows: ground corn (A, broken corn (B, ground sorghum (C, ground (50% and whole (50% sorghum (D and whole sorghum (100%; E. Pre-starter feeds (1 to 8 days, based on corn or sorghum, were produced only with ground grains. The study was divided in two trials: males and females. The results were submitted to analysis of variance (p<0.05 and Tukey's test. Feed intake, live weight, feed conversion ratio, and livability of 21- and 42-day-old male and female broilers were not affected by the treatments. This demonstrates that broiler performance is not influenced by sorghum physical form and that broilers fed sorghum-based diets presented similar performance as those fed corn-based diets. Gizzard and small intestine were heavier when whole sorghum grains was included in the diet. It was concluded that whole sorghum grains can be included in broiler feeds with not detrimental effects on performance.

  10. Good Grains Gone Bad: How Grain to Grain Interactions Complicate the Onset of Motion

    Science.gov (United States)

    Yager, E.; Schmeeckle, M. W.

    2015-12-01

    Predictions of the onset of sediment motion are integral components of bed stability and bedload flux estimates. Mechanistic equations for initial motion employ a balance between driving and resisting forces. Driving forces are modeled as functions of the magnitude and duration of turbulence events whereas resisting forces are simply approximated by the grain weight and a static friction angle. Such resistance approximations do not include the effects of grain packing and dynamic interactions with surrounding sediment. To better understand and quantify grain resistance, we used a Discrete Element Method (DEM) model for a single test grain surrounded by a bed of smaller grains. We applied a constant external force on the test grain in each run and progressively increased the force between runs until the test grain moved out of its resting pocket. The DEM model calculated the test grain velocity, position and net force (sum of applied external force and forces from other grains) at time steps of 1×10-7 s. Despite applying a constant external force, the net force on the test grain fluctuated by three to six orders of magnitude, depending on the run. These fluctuations were driven by the creation and destruction of force chains, and the rearrangement of the positions of surrounding bed sediment. Stick-slip behavior, which has been observed in shear tests of granular material, occurred during test-grain motion. The frequency of stick-slip behavior generally declined with higher applied external forces. Therefore, the onset of grain motion was not continuous, as is often assumed even in the presence of fluctuating applied fluid forces. The duration and magnitude of turbulence fluctuations have received considerable attention but our results suggest that grain resistance oscillations are also important. Whether turbulence and resistance fluctuations are synchronous will likely dictate if grain movement occurs, and we are currently conducting model runs to better

  11. Germinated grains: a superior whole grain functional food?

    Science.gov (United States)

    Nelson, Kristina; Stojanovska, Lily; Vasiljevic, Todor; Mathai, Michael

    2013-06-01

    Grains are global dietary staples that when consumed in whole grain form, offer considerable health benefits compared with milled grain foods, including reduced body weight gain and reduced cardiovascular and diabetes risks. Dietary patterns, functional foods, and other lifestyle factors play a fundamental role in the development and management of epidemic lifestyle diseases that share risks of developing adverse metabolic outcomes, including hyperglycaemia, hypertension, dyslipidaemia, oxidative stress, and inflammation. Whole grains provide energy, nutrients, fibres, and bioactive compounds that may synergistically contribute to their protective effects. Despite their benefits, the intake of grains appears to be lower than recommended in many countries. Of emerging interest is the application of germination processes, which may significantly enhance the nutritional and bioactive content of grains, as well as improve palatability. Enhancing grain foods in a natural way using germination techniques may therefore offer a practical, natural, dietary intervention to increase the health benefits and acceptability of whole grains, with potentially widespread effects across populations in attenuating adverse lifestyle disease outcomes. Continuing to build on the growing body of in-vitro studies requires substantiation with extended in-vivo trials so that we may further develop our understanding of the potential of germinated grains as a functional food.

  12. Variability of the Brewer-Dobson circulation's meridional and vertical branch using Aura/MLS water vapor

    Directory of Open Access Journals (Sweden)

    T. Flury

    2012-08-01

    Full Text Available We use Aura/MLS stratospheric water vapor measurements to infer interannual variations in the speed of the Brewer-Dobson circulation (BDC from 2004 to 2011. Stratospheric water vapor (H2O is utilized as a tracer for dynamics and we follow its path along the vertical and meridional branch of the BDC from the tropics to mid-latitudes. We correlate one year time series of H2O in the lower stratosphere at two subsequent altitude levels (68 hPa, ~18.8 km and 56 hPa, ~19.9 km at the Equator and determine the time lag for best correlation. The same calculation is made on the horizontal on the 100 hPa (~16.6 km level by correlating the H2O time series at the Equator with the ones at 40° N and 40° S. From these lag coefficients we derive the vertical and horizontal speeds of the BDC in the tropics and extra-tropics respectively. We observe a clear interannual variability of the vertical and horizontal branch. The variability reflects signatures of the Quasi Biennial Oscillation (QBO. Our measurements confirm the QBO meridional circulation anomalies and show that the speed variations in the two branches of the BDC are out of phase and fairly well anti-correlated. Maximum ascent rates are found during the QBO easterly phase. We also find that the transport towards the Northern Hemisphere (NH is on the average two times faster than to the Southern Hemisphere (SH with a mean speed of 1.15 m s−1 at 100 hPa. Furthermore, the speed towards the NH shows much more variability with an amplitude of about 21% whilst the speed towards the SH varies by only 10%. An amplitude of 21% is also observed in the variability of the ascent rate at the Equator which is on the average 0.2 mm s−1 and hence about 5000 times slower than the meridional branch.

  13. Grain charging in protoplanetary discs

    CERN Document Server

    Ilgner, Martin

    2011-01-01

    Recent work identified a growth barrier for dust coagulation that originates in the electric repulsion between colliding particles. Depending on its charge state, dust material may have the potential to control key processes towards planet formation such as MHD (magnetohydrodynamic) turbulence and grain growth which are coupled in a two-way process. We quantify the grain charging at different stages of disc evolution and differentiate between two very extreme cases: compact spherical grains and aggregates with fractal dimension D_f = 2. Applying a simple chemical network that accounts for collisional charging of grains, we provide a semi-analytical solution. This allowed us to calculate the equilibrium population of grain charges and the ionisation fraction efficiently. The grain charging was evaluated for different dynamical environments ranging from static to non-stationary disc configurations. The results show that the adsorption/desorption of neutral gas-phase heavy metals, such as magnesium, effects the ...

  14. Grain Handling and Storage Safety

    OpenAIRE

    Webster, Jill, Ph.D.

    2005-01-01

    Agricultural Health and Safety Fact Sheet AHS-02 Grain Handling and Storage Safety Jill Webster Ph.D., S. Christian Mariger, Graduate Assistant Agricultural Systems Technology and Education There are several hazards that should be considered when working with grain. Storage structures, handling equipment, and the grain itself have all caused serious injuries and deaths. Storage structures (bins, silos, and granaries), like all confined spaces, have significant hazards associated with them. Be...

  15. grain-filling, chlorophyll content in relation with grain yield ...

    African Journals Online (AJOL)

    Prof. Adipala Ekwamu

    The main effect of high temperatures during grain filling is to ... performance, is a crucial determinant of grain yield in cereal crops. ... 6 rows with 0.20 m row spacing, sowing density ... of wheat, corn (Zea mays L.), and other plants. (Wood et al.

  16. Study on adsorption of mixed heavy metal ions by discarded brewer's yeast%酿酒废酵母吸附共存重金属离子的研究

    Institute of Scientific and Technical Information of China (English)

    武运; 葛凤; 陈卫林; 聂威; 孙翠; 古丽娜孜

    2012-01-01

    To study the feasible for the discarded brewer's yeast adsorption heavy metal ions, on the basis of single factor experiment, use response surface method to make sure the best conditions for discarded brewer's yeast adsorption heavy metal ions. Result: the optimal adsorption conditions of metal ions by discarded brewer's yeast were; pH value is 3. 15, adsorption temperature is 30℃, adsorption time is 60 min, the density of discarded brewer's yeast is 2. 04 g/L and the density of metal ions is 61. 34 mg/L. Under the above conditions, the adsorption rate of metal ions by discarded brewer's yeast can approximately reach to 62. 78%.%为研究酿酒废酵母吸附共存重金属离子的可行性,在单因素试验的基础上,采用响应曲面法探讨酿酒废酵母吸附共存离子Pb2+、Ni2-、Cu2+的最佳工艺条件.结果表明:酿酒废酵母对共存重金属离子Pb2+、Ni2、Cu2+吸附的最佳条件为pH值3.15,吸附温度30℃,吸附时间60 min,酵母浓度2.04 g/L,离子浓度61.34mg/L,该条件下酿酒废酵母对共存离子的吸附率为62.78%.

  17. Effects of extruding wheat dried distillers grains with solubles with peas or canola meal on ruminal fermentation, microbial protein synthesis, nutrient digestion, and milk production in dairy cows.

    Science.gov (United States)

    Claassen, R M; Christensen, D A; Mutsvangwa, T

    2016-09-01

    Our objective was to examine the effects of feeding coextruded and nonextruded supplements consisting of wheat dried distillers grains with solubles with peas (WDDGS-peas) or canola meal (WDDGS-CM) on ruminal fermentation, omasal flow, and production performance in Holstein cows. Eight cows (4 ruminally cannulated) were used in a replicated 4×4 Latin square with 28-d periods and a 2×2 factorial arrangement of dietary treatments. Dietary treatments were coextruded or nonextruded mixtures of WDDGS-peas and WDDGS-CM that were included in total mixed rations at 15.1% [dry matter (DM) basis]. Diet had no effect on DM intake. Milk yield was greater in cows fed coextruded diets compared with those fed nonextruded diets. Milk fat content was greater in cows fed nonextruded diets compared with those fed coextruded diets, but milk fat yield was greater in cows fed coextruded diets compared with those fed nonextruded diets. Milk yield tended to be greater and milk protein yield was greater in cows fed WDDGS-peas compared with those fed WDDGS-CM. Cows fed nonextruded diets had a greater milk urea-N concentration compared with those fed coextruded diets. Cows fed coextruded diets had greater ruminal digestion of DM and tended to have greater ruminal digestion of organic matter compared with those fed nonextruded diets. Total-tract digestibilities of organic matter, crude protein, ether extract, and starch were greater, whereas that of acid detergent fiber and neutral detergent fiber tended to be greater in cows fed coextruded compared with those fed nonextruded diets. Total-tract digestibility of ether extract was lower whereas that of starch was greater and that of crude protein tended to be greater in cows fed WDDGS-peas compared with those fed WDDGS-CM. Total N excretion and milk N efficiency were unaffected by diet. Ruminal NH3-N concentration tended to be greater in cows fed WDDGS-CM compared with those fed WDDGS-peas. Ruminal propionate concentration was greater whereas

  18. Alignment of suprathermally rotating grains

    Science.gov (United States)

    Lazarian, A.

    1995-12-01

    It is shown that mechanical alignment can be efficient for suprathermally rotating grains, provided that they drift with supersonic velocities. Such a drift should be widely spread due to both Alfvenic waves and ambipolar diffusion. Moreover, if suprathermal rotation is caused by grain interaction with a radiative flux, it is shown that mechanical alignment may be present even in the absence of supersonic drift. This means that the range of applicability of mechanical alignment is wider than generally accepted and that it can rival the paramagnetic one. We also study the latter mechanism and re-examine the interplay between poisoning of active sites and desorption of molecules blocking the access to the active sites of H_2 formation, in order to explain the observed poor alignment of small grains and good alignment of large grains. To obtain a more comprehensive picture of alignment, we briefly discuss the alignment by radiation fluxes and by grain magnetic moments.

  19. Urbanization and Grain Production Efficiency

    Institute of Scientific and Technical Information of China (English)

    Xiaoyang; LI; Dongge; LIU

    2015-01-01

    Based on DEA-Malmquist method,this paper calculated the integrated technology efficiency of grain production and total factor productivity and analyzed factors influencing the grain production technology efficiency using working documents of panel structure. Research results indicate that grain production integrated technology efficiency of China is relatively low,technology utilization level is low,and it remains at the stage of decreasing returns to scale,and the pure technology efficiency still has space to increase. Total factor productivity is declining and the total factor productivity of many provinces is relatively low. Since the total factor productivity of eastern areas is higher than central and western areas,it is required to strengthen technological support for grain production. The implementation of urbanization is helpful for promoting increase of grain production technology efficiency in central and eastern areas,but it will exert negative influence on western areas.

  20. Autonomous grain combine control system

    Science.gov (United States)

    Hoskinson, Reed L.; Kenney, Kevin L.; Lucas, James R.; Prickel, Marvin A.

    2013-06-25

    A system for controlling a grain combine having a rotor/cylinder, a sieve, a fan, a concave, a feeder, a header, an engine, and a control system. The feeder of the grain combine is engaged and the header is lowered. A separator loss target, engine load target, and a sieve loss target are selected. Grain is harvested with the lowered header passing the grain through the engaged feeder. Separator loss, sieve loss, engine load and ground speed of the grain combine are continuously monitored during the harvesting. If the monitored separator loss exceeds the selected separator loss target, the speed of the rotor/cylinder, the concave setting, the engine load target, or a combination thereof is adjusted. If the monitored sieve loss exceeds the selected sieve loss target, the speed of the fan, the size of the sieve openings, or the engine load target is adjusted.

  1. Coarse Grained Quantum Dynamics

    CERN Document Server

    Agon, Cesar; Kasko, Skyler; Lawrence, Albion

    2014-01-01

    We consider coarse graining a quantum system divided between short distance and long distance degrees of freedom, which are coupled by the Hamiltonian. Observations using purely long distance observables can be described by the reduced density matrix that arises from tracing out the short-distance observables. The dynamics of this density matrix is that of an open quantum system, and is nonlocal in time, on the order of some short time scale. We describe these dynamics in a model system with a simple hierarchy of energy gaps $\\Delta E_{UV} > \\Delta E_{IR}$, in which the coupling between high-and low-energy degrees of freedom is treated to second order in perturbation theory. We then describe the equations of motion under suitable time averaging, reflecting the limited time resolution of actual experiments, and find an expansion of the master equation in powers of $\\Delta E_{IR}/\\Delta E_{UV}$, in which the failure of the system to be Hamiltonian or even Markovian appears at higher orders in this ratio. We com...

  2. Chemical evaluation and digestibility of cocoa (Theobroma cacao) byproducts fed to goats.

    Science.gov (United States)

    Aregheore, E M

    2002-07-01

    A study was undertaken to evaluate and compare the chemical composition of the cocoa byproducts CPH (cocoa pod husk), CS (cocoa shell) and CD (cocoa dust), and to establish a rational use of CS and CD in the diets of growing goats. CD had a high crude protein (CP) content of 15.9%, while CS and CPH had 13.8% and 6.7%, respectively. The byproducts were high in crude fibre (CF) content. Among the byproducts, CD had the highest ether extract value (22.0%). Fifteen growing goats, 18-20 months of age, with pre-experimental body weights of 20.9 +/- 0.33 kg, were randomly allotted to three diets in growth studies. In diet 1, dried brewers' grain (DBG) served as the control, while the other two diets had CS or CD plus DBG. The dry matter intake (DMI) was 570, 530 and 486 g/head per day for the control, CS + DBG and CD + DBG diets, respectively. The growth rate differed significantly among the goats offered the diets (p < 0.05). Dry matter (DM), crude protein (CP), crude fibre (CF), organic matter (OM) and gross energy (GE) digestibility were significantly higher (p < 0.05) in the goats on the control diet than in those on CS + DBG or CD + DBG. The DM, CP and OM in the CS + DBG diet were more digestible (p < 0.05) than those in the CD + DBG diet. The inclusion of DBG in the CS and CD diets improved their use by the goats.

  3. Modeling brewers' yeast flocculation

    Science.gov (United States)

    van Hamersveld EH; van der Lans RG; Caulet; Luyben

    1998-02-01

    Flocculation of yeast cells occurs during the fermentation of beer. Partway through the fermentation the cells become flocculent and start to form flocs. If the environmental conditions, such as medium composition and fluid velocities in the tank, are optimal, the flocs will grow in size large enough to settle. After settling of the main part of the yeast the green beer is left, containing only a small amount of yeast necessary for rest conversions during the next process step, the lagering. The physical process of flocculation is a dynamic equilibrium of floc formation and floc breakup resulting in a bimodal size distribution containing single cells and flocs. The floc size distribution and the single cell amount were measured under the different conditions that occur during full scale fermentation. Influences on flocculation such as floc strength, specific power input, and total number of yeast cells in suspension were studied. A flocculation model was developed, and the measured data used for validation. Yeast floc formation can be described with the collision theory assuming a constant collision efficiency. The breakup of flocs appears to occur mainly via two mechanisms, the splitting of flocs and the erosion of yeast cells from the floc surface. The splitting rate determines the average floc size and the erosion rate determines the number of single cells. Regarding the size of the flocs with respect to the scale of turbulence, only the viscous subrange needs to be considered. With the model, the floc size distribution and the number of single cells can be predicted at a certain point during the fermentation. For this, the bond strength between the cells, the fractal dimension of the yeast, the specific power input in the tank and the number of yeast cells that are in suspension in the tank have to be known. Copyright 1998 John Wiley & Sons, Inc.

  4. MATHEMATICAL MODEL OF GRAIN MICRONIZATION

    Directory of Open Access Journals (Sweden)

    V. A. Afanas’ev

    2014-01-01

    Full Text Available Summary. During micronisation grain moisture evaporates mainly in decreasing drying rate period. Grain layer located on the surface of the conveyor micronisers will be regarded as horizontal plate. Due to the fact that the micronisation process the surface of the grain evaporates little moisture (within 2-7 % is assumed constant plate thickness. Because in the process of micronization grain structure is changing, in order to achieve an exact solution of the equations necessary to take into account changes thermophysical, optical and others. Equation of heat transfer is necessary to add a term that is responsible for the infrared heating. Because of the small thickness of the grain, neglecting the processes occurring at the edge of the grain, that is actually consider the problem of an infinite plate. To check the adequacy of the mathematical model of the process of micronisation of wheat grain moisture content must be comparable to the function of time, obtained by solving the system of equations with the measured experimental data of experience. Numerical solution of a system of equations for the period of decreasing drying rate is feasible with the help of the Maple 14, substituting the values of the constants in the system. Calculation of the average relative error does not exceed 7- 10 %, and shows a good agreement between the calculated data and the experimental values.

  5. Quantitative characterisation of sedimentary grains

    Science.gov (United States)

    Tunwal, Mohit; Mulchrone, Kieran F.; Meere, Patrick A.

    2016-04-01

    Analysis of sedimentary texture helps in determining the formation, transportation and deposition processes of sedimentary rocks. Grain size analysis is traditionally quantitative, whereas grain shape analysis is largely qualitative. A semi-automated approach to quantitatively analyse shape and size of sand sized sedimentary grains is presented. Grain boundaries are manually traced from thin section microphotographs in the case of lithified samples and are automatically identified in the case of loose sediments. Shape and size paramters can then be estimated using a software package written on the Mathematica platform. While automated methodology already exists for loose sediment analysis, the available techniques for the case of lithified samples are limited to cases of high definition thin section microphotographs showing clear contrast between framework grains and matrix. Along with the size of grain, shape parameters such as roundness, angularity, circularity, irregularity and fractal dimension are measured. A new grain shape parameter developed using Fourier descriptors has also been developed. To test this new approach theoretical examples were analysed and produce high quality results supporting the accuracy of the algorithm. Furthermore sandstone samples from known aeolian and fluvial environments from the Dingle Basin, County Kerry, Ireland were collected and analysed. Modern loose sediments from glacial till from County Cork, Ireland and aeolian sediments from Rajasthan, India have also been collected and analysed. A graphical summary of the data is presented and allows for quantitative distinction between samples extracted from different sedimentary environments.

  6. An Improved Implementation of Grain

    CERN Document Server

    Mansouri, Shohreh Sharif

    2009-01-01

    A common approach to protect confidential information is to use a stream cipher which combines plain text bits with a pseudo-random bit sequence. Among the existing stream ciphers, Non-Linear Feedback Shift Register (NLFSR)-based ones provide the best trade-off between cryptographic security and hardware efficiency. In this paper, we show how to further improve the hardware efficiency of Grain stream cipher. By transforming the NLFSR of Grain from its original Fibonacci configuration to the Galois configuration and by introducing a clock division block, we double the throughput of the 80 and 128-bit key 1bit/cycle architectures of Grain with no area penalty.

  7. Analysis of variability, heritability, and interrelationships among grain yield and related characters in barley advanced lines

    Directory of Open Access Journals (Sweden)

    Ahmadi Jafar

    2016-01-01

    Full Text Available The main goal of this study was to improve pure lines for low input agricultural areas of the semi-arid region of Iran and similar environmental conditions. Forty barley pure lines provided from ICARDA along with three check cultivars were evaluated under rain-fed conditions in a semi-arid region of Iran during two years (2011-2013. The experiments were conducted in a randomized complete block design with four replications to estimate genetic variation and heritability for agro-morphological traits. The highest values of phenotypic and genotypic coefficients of variation were observed for the number of grains per spike followed by peduncle length, early vigor and grain yield. The broad heritability estimates ranged from 24% for grain yield to 96% for the number of grains per spike. The highest and lowest expected genetic advance, observed for days to physiological maturity and the number of grains per spike, respectively. Results of stepwise regression and path analysis showed that hectoliter grain weight and thousand grain weight exhibited the highest direct effects on grain yield, respectively. Comparisons between the pure lines and check cultivars indicated that out of 40 pure lines, 27 lines had a higher grain yield. Also, the pure lines No. 29, 13, 9 and 33 were identified as the superior lines for semi-arid environmental conditions. Our results indicate that check cultivars could be improved by selecting for pure lines with taller peduncle and the number of grains per spike, but with heavier grains. Therefore, these lines can be used as genetic material to broaden the genetic basis of barley breeding programs all over the world.

  8. PARASITE MYCOPOPULATION OF SOYBEAN GRAIN

    Directory of Open Access Journals (Sweden)

    Jasenka Ćosić

    2008-07-01

    Full Text Available Disease appearance on soybean can influence quality and quantity of yield. Different spieces of saprophyte and parasite fungi can be isolated from stems, pods and grain of soybean. The aim of the research was to evaluate the incidence of important disease on natural soybean grain over the period of 4 years (2004-2007 of experiment held on the location Sopot-Vinkovci and included 9 cultivars of soybean. The following plant pathogenic fungi were identified: Peronospora, Sclerotinia, Cercospora, Fusarium and Diaporthe/Phomopsis. The most frequent fungi on soybean grains were: Cladosporium, Alternaria, Penicillium, Aspergillus and Epicoccum. The health condition of the natural soybean grains over the four years period on all cultivars was good.

  9. Export Rebates on Grain Finished

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ According to the State Statistics Bureau, China's consumer price index (CPI) jumped by 6.9 percent year-on-year last November, approaching the high levels seen at the end of 1996, one of the largest areas of increase was the price of grain,which rose by 6.6 percent. There is no doubt the growth in CPI can be mainly attributed to soaring food prices and the diffusion effect of oil price adjustments. Large orders for grain exports have put Chinese grain suppliers under tremendous pressure. In order to solve this problem, the Finance Ministry and State Administration of Taxation together announced that 84 categories of export tax rebates on major types of grain will be eliminated.

  10. Export Rebates on Grain Finished

    Institute of Scientific and Technical Information of China (English)

    Liu; Xinwen

    2008-01-01

      According to the State Statistics Bureau, China's consumer price index (CPI) jumped by 6.9 percent year-on-year last November, approaching the high levels seen at the end of 1996, one of the largest areas of increase was the price of grain,which rose by 6.6 percent. There is no doubt the growth in CPI can be mainly attributed to soaring food prices and the diffusion effect of oil price adjustments. Large orders for grain exports have put Chinese grain suppliers under tremendous pressure. In order to solve this problem, the Finance Ministry and State Administration of Taxation together announced that 84 categories of export tax rebates on major types of grain will be eliminated.……

  11. Selection of barley grain affects ruminal fermentation, starch digestibility, and productivity of lactating dairy cows.

    Science.gov (United States)

    Silveira, C; Oba, M; Yang, W Z; Beauchemin, K A

    2007-06-01

    The objective of this study was to evaluate the effects of 2 lots of barley grain cultivars differing in expected ruminal starch degradation on dry matter (DM) intake, ruminal fermentation, ruminal and total tract digestibility, and milk production of dairy cows when provided at 2 concentrations in the diet. Four primiparous ruminally cannulated (123 +/- 69 d in milk; mean +/- SD) and 4 multiparous ruminally and duodenally cannulated (46 +/- 14 d in milk) cows were used in a 4 x 4 Latin Square design with a 2 x 2 factorial arrangement of treatments with 16-d periods. Primiparous and multiparous cows were assigned to different squares. Treatments were 2 dietary starch concentrations (30 vs. 23% of dietary DM) and 2 lots of barley grain cultivars (Xena vs. Dillon) differing in expected ruminal starch degradation. Xena had higher starch concentration (58.7 vs. 50.0%) and greater in vitro 6-h starch digestibility (78.0 vs. 73.5%) compared with Dillon. All experimental diets were formulated to supply 18.3% crude protein and 20.0% forage neutral detergent fiber. Dry matter intake and milk yield were not affected by treatment. Milk fat concentration (3.55 vs. 3.29%) was greater for cows fed Dillon compared with Xena, but was not affected by dietary starch concentration. Ruminal starch digestion was greater for cows fed high-starch diets compared with those fed low-starch diets (4.55 vs. 2.49 kg/d), and tended to be greater for cows fed Xena compared with those fed Dillon (3.85 vs. 3.19 kg/d). Ruminal acetate concentration was lower, and propionate concentration was greater, for cows fed Xena or high-starch diets compared with cows fed Dillon or low-starch diets, respectively. Furthermore, cows fed Xena or high-starch diets had longer duration that ruminal pH was below 5.8 (6.6 vs. 4.0 and 6.4 vs. 4.2 h/d) and greater total tract starch digestibility (94.3 vs. 93.0 and 94.3 vs. 93.0%) compared with cows fed Dillon or low-starch diets, respectively. These results

  12. PARASITE MYCOPOPULATION OF SOYBEAN GRAIN

    OpenAIRE

    Jasenka Ćosić; Karolina Vrandečić; Draženka Jurković; Ivan Ereš; Jelena Poštić

    2008-01-01

    Disease appearance on soybean can influence quality and quantity of yield. Different spieces of saprophyte and parasite fungi can be isolated from stems, pods and grain of soybean. The aim of the research was to evaluate the incidence of important disease on natural soybean grain over the period of 4 years (2004-2007) of experiment held on the location Sopot-Vinkovci and included 9 cultivars of soybean. The following plant pathogenic fungi were identified: Peronospora, Sclerotinia, Cercospora...

  13. Superconductivity of small metal grains

    Institute of Scientific and Technical Information of China (English)

    ZHENG; Renrong; CHEN; Zhiqian; ZHU; Shunquan

    2005-01-01

    The formulas of the energy gap and superconducting critical temperature appropriate for systems with both odd and even number of electrons are derived; the bases of the derivations are BCS theory and energy level statistics. Numerical results qualitatively agree with the experimental phenomena. i.e., the superconductivity of small metallic grains will first enhance then decrease to zero when the grain are getting smaller and smaller. The calculations indicate that the above phenomena happen in the metallic grains belonging to Gaussian Orthogonal Ensemble (GOE) and Gaussian Unitary ensemble (GUE) with zero spin; The superconductivity of small metallic grains in Gaussian Symplectic Ensemble (GSE) will monotonically decrease to zero with the decreasing of the grain size. The analyses suggest that the superconductivity enhancements come from pairing and the balance of the strengths between spin-orbital coupling and external magnetic field. In order to take the latter into account, it is necessary to include the level statistics given by Random Matrix Theory (RMT) in describing small metallic grains.

  14. Relationship of Breast-fed and Bottle-fed First Grade Students and I.Q.

    Science.gov (United States)

    Humphrey, Danette

    Previous studies have indicated some support for the hypothesis that breast feeding has a positive effect on intelligence and attainment among young children. This study examined the effects of breast-feeding versus bottle-feeding on the intelligence quotients (IQs) of first graders. A total of 26 breast-fed and 26 bottle-fed first graders from an…

  15. Evaluation of corn distillers dried grains with solubles as an alternative ingredient for broilers.

    Science.gov (United States)

    Shim, M Y; Pesti, G M; Bakalli, R I; Tillman, P B; Payne, R L

    2011-02-01

    The effects of graded levels of corn distillers dried grains with solubles (DDGS) were investigated as a partial replacement for sources of protein, energy, and other nutrients for broilers when the digestible amino acid balance was maintained. Zero, 8, 16, and 24% DDGS were incorporated into isonutritive diets at the expense of corn, soybean meal, and dl-Met. Poultry oil, l-Lys, and l-Thr additions increased with increasing levels of DDGS. Diets were each fed to 36 Cobb 500 straight-run broilers in 6 floor pens in 2 experiments. In experiment 1, broilers fed ≥8% DDGS showed increased BW gain compared with those fed the control diet during the 0- to 18-d starter period (P = 0.0164) but were almost identical in BW at 42 d (P = 0.9395). The only difference at 42 d was in the carcass fat composition of female broilers: percentage of fat pad decreased with increasing DDGS level (P = 0.0133). Corn DDGS reduced the pellet durability index. However, the pellet durability index was not related to growth or feed utilization. In experiment 2 at 42 d, broilers fed all levels of DDGS showed increased BW gain compared with those fed the control diet. Broilers may perform well when fed properly balanced feeds containing up to 24% DDGS despite reduced pellet quality.

  16. Proceedings of FED remote maintenance equipment workshop

    Energy Technology Data Exchange (ETDEWEB)

    Sager, P.; Garin, J.; Hager, E.R.; Spampinato, P.T.; Tobias, D.; Young, N.

    1981-11-01

    A workshop was convened in two sessions in January and March 1981, on the remote maintenance equipment for the Fusion Engineering Device (FED). The objectives of the first session were to familiarize the participants with the status of the design of the FED and to develop a remote maintenance equipment list for the FED. The objective of the second session was to have the participants present design concepts for the equipment which had been identified in the first session. The equipment list was developed for general purpose and special purpose equipment. The general purpose equipment was categorized as manipulators and other, while the special purpose equipment was subdivided according to the reactor subsystem it serviced: electrical, magnetic, and nuclear. Both mobile and fixed base manipulators were identified. Handling machines were identified as the major requirement for special purpose equipment.

  17. [Digestibility of columnar cacti pollen grains in the glosophagine bats Glossophaga longirostris and Leptonycteris curasoae (Chiroptera: Phyllostomidae)].

    Science.gov (United States)

    Muñoz-Romo, Mariana; Sosa, Maricela; Quintero, Yveth Casart

    2005-01-01

    We examined the protoplasmic assimilation of columnar cacti pollen grains in two species of Venezuelan desert glosophagine bats, Glossophaga longirostris and Leptonycteris curasoae, by determining the amount of empty (digested) pollen grains found in their fecal samples. To determine the amount of empty pollen grains, the fecal samples were stained to differ between empty and full (non-digested) pollen grains. The number of empty and full pollen grains observed in the fecal samples were corrected using the amount of aborted pollens present (before anthesis) in flowers of the columnar cacti species (Subpilocereus repandus, Stenocereus griseus and Pilosocereus tillianus) used by bats as food in the study site; G. longirostris and L. curasoae digested 64.2% and 71.3% of all the pollens fed, respectively. These high values confirm the importance of pollen in the diet of these bats, given its high nitrogen level.

  18. Grain dynamics in zero gravity

    Science.gov (United States)

    Werner, B. T.; Haff, P. K.

    1987-01-01

    The dynamics of granular materials has proved difficult to model, primarily because of the complications arising from inelastic losses, friction, packing, and the effect of many grains being in contact simultaneously. One interesting limit for which it was recently possible to construct a theory is that where the grain-grain interactions are dominated by binary collisions. The kinetic model of granular systems if similar to the kinetic theory of gases, except that collisional energy losses are always present in the former and must be treated explicitly. Few granular materials on Earth are describable by this limiting model, since gravity tends to collapse the grains into a high-density state where Coulombic friction effects are dominant. The planned Space Station offers an unusual opportunity to test the kinetic grain model and to explore its predictions. Without gravity, the regime of low interparticle velocities, where an elastic description of the collision is still valid, is investigated. This will allow direct interpretation by dynamical computer simulations as well as by kinetic theory.

  19. Deformation strain inhomogeneity in columnar grain nickel

    DEFF Research Database (Denmark)

    Wu, G.L.; Godfrey, A.; Juul Jensen, D.

    2005-01-01

    A method is presented for determination of the local deformation strain of individual grains in the bulk of a columnar grain sample. The method, based on measurement of the change in grain area of each grain, is applied to 12% cold rolled nickel. Large variations are observed in the local strain...

  20. Abnormal grain growth: a non-equilibrium thermodynamic model for multi-grain binary systems

    Science.gov (United States)

    Svoboda, J.; Fischer, F. D.

    2014-01-01

    Abnormal grain growth as the abrupt growth of a group of the largest grains in a multi-grain system is treated within the context of unequal retardation of grain growth due to the segregation of solute atoms from the bulk of the grains into the grain boundaries. During grain boundary migration, the segregated solute atoms are dragged under a small driving force or left behind the migrating grain boundary under a large driving force. Thus, the solute atoms in the grain boundaries of large grains, exhibiting a large driving force, can be released from the grain boundary. The mobility of these grain boundaries becomes significantly higher and abnormal grain growth is spontaneously provoked. The mean-field model presented here assumes that each grain is described by its grain radius and by its individual segregation parameter. The thermodynamic extremal principle is engaged to obtain explicit evolution equations for the radius and segregation parameter of each grain. Simulations of grain growth kinetics for various conditions of segregation with the same initial setting (100 000 grains with a given radius distribution) are presented. Depending on the diffusion coefficients of the solute in the grain boundaries, abnormal grain growth may be strongly or marginally pronounced. Solute segregation and drag can also significantly contribute to the stabilization of the grain structure. Qualitative agreement with several experimental results is reported.

  1. Sticking properties of ice grains

    Directory of Open Access Journals (Sweden)

    Jongmanns M.

    2017-01-01

    Full Text Available We study the size dependence of pull-off forces of water ice in laboratory experiments and numerical simulations. To determine the pull-off force in our laboratory experiments, we use a liquid nitrogen cooled centrifuge. Depending on its rotation frequency, spherical ice grains detach due to the centrifugal force which is related to the adhesive properties. Numerical simulations are conducted by means of molecular dynamics simulations of hexagonal ice using a standard coarse-grained water potential. The pull-off force of a single contact between two spherical ice grains is measured due to strain controlled simulations. Both, the experimental study and the simulations reveal a dependence between the pull-off force and the (reduced particle radii, which differ significantly from the linear dependence of common contact theories.

  2. Interstellar Grains: 50 Years On

    CERN Document Server

    Wickramasinghe, N Chandra

    2011-01-01

    Our understanding of the nature of interstellar grains has evolved considerably over the past half century with the present author and Fred Hoyle being intimately involved at several key stages of progress. The currently fashionable graphite-silicate-organic grain model has all its essential aspects unequivocally traceable to original peer-reviewed publications by the author and/or Fred Hoyle. The prevailing reluctance to accept these clear-cut priorities may be linked to our further work that argued for interstellar grains and organics to have a biological provenance - a position perceived as heretical. The biological model, however, continues to provide a powerful unifying hypothesis for a vast amount of otherwise disconnected and disparate astronomical data.

  3. Bioactive compounds in whole grain wheat

    OpenAIRE

    Mateo Anson, N.

    2010-01-01

    Bread can be healthier! Consuming whole-grain foods can prevent cardiovascular diseases, type-2 diabetes and metabolic syndrome. This is due to bioactive compounds in whole grain, such as antioxidants and anti-inflammatory compounds. We found that the different fractions of a wheat grain vary much in their content. The external fractions of the grain, the bran and specially the aleurone, are the richest. We observed that processing the bran in whole-grain breads increased three times the leve...

  4. PESTICIDES USE AMONG GRAIN MERCHANTS IN MUBI GRAIN ...

    African Journals Online (AJOL)

    AGROSEARCH UIL

    have: low level of education, while (30%) of them have no formal education. ... various techniques to ensure that the quality of the stored grains does not deteriorate ... education level, period of storage, technical knowledge on how to apply the ...

  5. Grain centre mapping - 3DXRD measurements of average grain characteristics

    DEFF Research Database (Denmark)

    Oddershede, Jette; Schmidt, Søren; Lyckegaard, Allan;

    2014-01-01

    Three-Dimensional X-ray Diraction (3DXRD) Microscopy is a generic term covering a variety of dierent techniques for characterising the mi- crostructure within the bulk of polycrystalline materials. One strategy | namely grain centre mapping | enables fast measurements of the av- erage characteris...

  6. FedEX Sponsors China's Badminton Team

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The reigning world champion team joins hands with the express delivery service to greet the coming Olympics Starting this year, Olympic badminton champion Zhang Ning and world badminton champion Lin Dan will be wearing clothing with the FedEx logo on it.

  7. Wideband electromagnetically coupled coaxial probe fed slot ...

    African Journals Online (AJOL)

    user

    ... and low gain that limits its application in practice (Bahl et al, 1980 and Balanis, ... distribution in upper and lower patches at both the resonance frequencies is .... The equivalent circuit of rectangular patch stacked with U-slot loaded fed patch ...

  8. Supervision of Fed-Batch Fermentations

    DEFF Research Database (Denmark)

    Gregersen, Lars; Jørgensen, Sten Bay

    1999-01-01

    Process faults may be detected on-line using existing measurements based upon modelling that is entirely data driven. A multivariate statistical model is developed and used for fault diagnosis of an industrial fed-batch fermentation process. Data from several (25) batches are used to develop a mo...

  9. Applied Thermodynamics: Grain Boundary Segregation

    Directory of Open Access Journals (Sweden)

    Pavel Lejček

    2014-03-01

    Full Text Available Chemical composition of interfaces—free surfaces and grain boundaries—is generally described by the Langmuir–McLean segregation isotherm controlled by Gibbs energy of segregation. Various components of the Gibbs energy of segregation, the standard and the excess ones as well as other thermodynamic state functions—enthalpy, entropy and volume—of interfacial segregation are derived and their physical meaning is elucidated. The importance of the thermodynamic state functions of grain boundary segregation, their dependence on volume solid solubility, mutual solute–solute interaction and pressure effect in ferrous alloys is demonstrated.

  10. Suitability of sorghum grain for the development of the larger grain ...

    African Journals Online (AJOL)

    Suitability of sorghum grain for the development of the larger grain borer ... on sorghum grain, when stored as whole grain or finely ground grain flour. ... In a similar manner, the mean weight of beetles produced ranged from 1.70 to 3.02 mg.

  11. 75 FR 81965 - Grain Inspection Advisory Committee Reestablishment

    Science.gov (United States)

    2010-12-29

    ... Grain Inspection, Packers and Stockyards Administration Grain Inspection Advisory Committee Reestablishment AGENCY: Grain Inspection, Packers and Stockyards Administration, USDA. ACTION: Notice to... Grain Inspection, Packers and Stockyards Administration (GIPSA) Grain Inspection Advisory Committee...

  12. 7 CFR 868.310 - Grades and grade requirements for the classes Long Grain Milled Rice, Medium Grain Milled Rice...

    Science.gov (United States)

    2010-01-01

    ... Grain Milled Rice, Medium Grain Milled Rice, Short Grain Milled Rice, and Mixed Milled Rice. (See also Â...) GRAIN INSPECTION, PACKERS AND STOCKYARD ADMINISTRATION (FEDERAL GRAIN INSPECTION SERVICE), DEPARTMENT OF... classes Long Grain Milled Rice, Medium Grain Milled Rice, Short Grain Milled Rice, and Mixed Milled Rice...

  13. Stress-driven grain growth

    CSIR Research Space (South Africa)

    Nabarro, FRN

    1998-11-13

    Full Text Available This article shows the differences of two-dimensional polycrystal of a material with a rectangular unit cell with lattice spacing b and b (1 + epsilon), subjected to a uniform external stress sigma. Consider a grain in which the lattice vector...

  14. AGB stars and presolar grains

    CERN Document Server

    Busso, M; Maiorca, E; Palmerini, S

    2013-01-01

    Among presolar materials recovered in meteorites, abundant SiC and Al$_{2}$O$_{3}$ grains of AGB origins were found. They showed records of C, N, O, $^{26}$Al and s-element isotopic ratios that proved invaluable in constraining the nucleosynthesis models for AGB stars \\cite{zin,gal}. In particular, when these ratios are measured in SiC grains, they clearly reveal their prevalent origin in cool AGB circumstellar envelopes and provide information on both the local physics and the conditions at the nucleosynthesis site (the H- and He-burning layers deep inside the structure). Among the properties ascertained for the main part of the SiC data (the so-called {\\it mainstream} ones), we mention a large range of $^{14}$N/$^{15}$N ratios, extending below the solar value \\cite{mar}, and $^{12}$C/$^{13}$C ratios $\\gtrsim$ 30. Other classes of grains, instead, display low carbon isotopic ratios ($\\gtrsim 10$) and a huge dispersion for N isotopes, with cases of large $^{15}$N excess. In the same grains, isotopes currently...

  15. Concepts on Low Temperature Mechanical Grain Growth

    Energy Technology Data Exchange (ETDEWEB)

    Sharon, John Anthony [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Metallurgy and Materials Joining Dept.; Boyce, Brad Lee [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Metallurgy and Materials Joining Dept.

    2013-11-01

    In metals, as grain size is reduced below 100nm, conventional dislocation plasticity is suppressed resulting in improvements in strength, hardness, and wears resistance. Existing and emerging components use fine grained metals for these beneficial attributes. However, these benefits can be lost in service if the grains undergo growth during the component’s lifespan. While grain growth is traditionally viewed as a purely thermal process that requires elevated temperature exposure, recent evidence shows that some metals, especially those with nanocrystalline grain structure, can undergo grain growth even at room temperature or below due to mechanical loading. This report has been assembled to survey the key concepts regarding how mechanical loads can drive grain coarsening at room temperature and below. Topics outlined include the atomic level mechanisms that facilitate grain growth, grain boundary mobility, and the impact of boundary structure, loading scheme, and temperature.

  16. Higher lipid accumulation in broilers fed on saturated fats than in those fed on unsaturated fats.

    Science.gov (United States)

    Sanz, M; Flores, A; De Ayala, P P; Lopez-Bote, C J

    1999-03-01

    1. Two experiments were conducted to assess the effect of fat sources differing in degree of saturation on the performance of and fat deposition in broiler chickens fed on isocaloric and isonitrogenous diets. 2. There were no differences in initial body weight between sexes but female broilers had lower daily gains (Psaturation. Broilers fed on diets containing an animal fat blend or tallow had higher abdominal fat pad weight (Psaturation affects fat accumulation in broiler chickens.

  17. Grain Refinement of Deoxidized Copper

    Science.gov (United States)

    Balart, María José; Patel, Jayesh B.; Gao, Feng; Fan, Zhongyun

    2016-10-01

    This study reports the current status of grain refinement of copper accompanied in particular by a critical appraisal of grain refinement of phosphorus-deoxidized, high residual P (DHP) copper microalloyed with 150 ppm Ag. Some deviations exist in terms of the growth restriction factor ( Q) framework, on the basis of empirical evidence reported in the literature for grain size measurements of copper with individual additions of 0.05, 0.1, and 0.5 wt pct of Mo, In, Sn, Bi, Sb, Pb, and Se, cast under a protective atmosphere of pure Ar and water quenching. The columnar-to-equiaxed transition (CET) has been observed in copper, with an individual addition of 0.4B and with combined additions of 0.4Zr-0.04P and 0.4Zr-0.04P-0.015Ag and, in a previous study, with combined additions of 0.1Ag-0.069P (in wt pct). CETs in these B- and Zr-treated casts have been ascribed to changes in the morphology and chemistry of particles, concurrently in association with free solute type and availability. No further grain-refining action was observed due to microalloying additions of B, Mg, Ca, Zr, Ti, Mn, In, Fe, and Zn (~0.1 wt pct) with respect to DHP-Cu microalloyed with Ag, and therefore are no longer relevant for the casting conditions studied. The critical microalloying element for grain size control in deoxidized copper and in particular DHP-Cu is Ag.

  18. AGB stars and presolar grains

    Energy Technology Data Exchange (ETDEWEB)

    Busso, M.; Trippella, O. [INFN and University of Perugia, Perugia (Italy); Maiorca, E. [INAF - Arcetri Astrophysical Observatory, Firenze, Italy and INFN - Section of Perugia, Perugia (Italy); Palmerini, S. [Departamento de Fìsica Teòrica y del Cosmsos, Universidad de Granada, Granada (Spain)

    2014-05-09

    Among presolar materials recovered in meteorites, abundant SiC and Al{sub 2}O{sub 3} grains of AGB origins were found. They showed records of C, N, O, {sup 26}Al and s-element isotopic ratios that proved invaluable in constraining the nucleosynthesis models for AGB stars [1, 2]. In particular, when these ratios are measured in SiC grains, they clearly reveal their prevalent origin in cool AGB circumstellar envelopes and provide information on both the local physics and the conditions at the nucleosynthesis site (the H- and He-burning layers deep inside the structure). Among the properties ascertained for the main part of the SiC data (the so-called mainstream ones), we mention a large range of {sup 14}N/{sup 15}N ratios, extending below the solar value [3], and {sup 12}C/{sup 13}C ratios ≳ 30. Other classes of grains, instead, display low carbon isotopic ratios (≳ 10) and a huge dispersion for N isotopes, with cases of large {sup 15}N excess. In the same grains, isotopes currently feeded by slow neutron captures reveal the characteristic pattern expected from this process at an efficiency slightly lower than necessary to explain the solar main s-process component. Complementary constraints can be found in oxide grains, especially Al{sub 2}O{sub 3} crystals. Here, the oxygen isotopes and the content in {sup 26}Al are of a special importance for clarifying the partial mixing processes that are known to affect evolved low-mass stars. Successes in modeling the data, as well as problems in explaining some of the mentioned isotopic ratios through current nucleosynthesis models are briefly outlined.

  19. Cool Down!——The State Administration of Grain Lower Expectation of Grain Price

    Institute of Scientific and Technical Information of China (English)

    He Jun

    2010-01-01

    @@ The rising grain price in the first half of the year,coupled with the news that China's summer grain output fell for the first time in seven years,has enhanced market expectation for the grain price to rise.

  20. Effects of urban agglomeration on surface-UV doses: a comparison of Brewer measurements in Warsaw and Belsk, Poland, for the period 2013-2015

    Science.gov (United States)

    Czerwińska, Agnieszka E.; Krzyścin, Janusz W.; Jarosławski, Janusz; Posyniak, Michał

    2016-11-01

    Specific aerosols and cloud properties over large urban regions seem to generate an island, similar to the well-known urban heat island, leading to lower ultraviolet (UV) radiation intensity compared to the surrounding less polluted areas, thus creating a shield against excessive human exposure to UV radiation. The present study focuses on differences between erythemal and UVA (324 nm) doses measured by the Brewer spectrophotometers in Warsaw (52.3° N, 21.0° E) and Belsk (51.8° N, 20.8° E). The latter is a rural region located about 60 km south-west of the city. Ratios between erythemal and UVA partly daily doses, obtained during all-sky and cloudless-sky conditions for the period May 2013-December 2015, were analysed to infer a specific cloud and aerosol forcing on the surface UV doses over Warsaw. Radiative model simulations were carried out to find sources of the observed differences between the sites. It was found that Warsaw urban agglomeration induced 8 and 6 % attenuation of the erythemal and UVA doses respectively. This is mostly due to the lower sun elevation in Warsaw during the near-noon measurements and the larger optical depth of the city aerosols and increased cloudiness. It could be hypothesised that the expected stronger absorption of the solar UV radiation by urban aerosols is compensated for here by a higher surface reflectivity over the city.

  1. Brewers' yeast pyruvate decarboxylase produces acetoin from acetaldehyde: a novel tool to study the mechanism of steps subsequent to carbon dioxide loss.

    Science.gov (United States)

    Chen, G C; Jordan, F

    1984-07-31

    A gas-liquid chromatographic technique was developed for the determination of both acetaldehyde and the 3-4% acetoin side product that results from the brewers' yeast pyruvate decarboxylase (EC 4.1.1.1) catalyzed reaction of pyruvic acid. Employing this method enabled the demonstration of the catalysis of acetaldehyde condensation to acetoin by the enzyme. It was found that the acetoin produced enzymatically from pyruvic acid or from acetaldehyde was optically active, thus providing stereochemical information about the reaction. Deuterium kinetic isotope effects (employing CH3CHO and CH3CDO) were determined on the steady-state kinetic parameters to be 4.5 (Vmax) and 3.2 (Vmax/Kappm), respectively. This enabled, for the first time, the estimation of relative kinetic barriers for steps past decarboxylation. It could be concluded that (a) C-H bond scission was part of rate limitation in the enzyme-catalyzed condensation of acetaldehyde to acetoin and that (b) among the steps leading to the release of acetaldehyde, protonation of the key enamine intermediate was part of rate limitation. This latter finding is also directly applicable to the mechanism of pyruvate decarboxylation.

  2. Effects of colupulone, a component of hops and brewers yeast, and chromium on glucose tolerance and hepatic cytochrome P450 in nondiabetic and spontaneously diabetic mice.

    Science.gov (United States)

    Mannering, G J; Shoeman, J A; Shoeman, D W

    1994-05-16

    Brewers yeast contains factors that increase and decrease glucose tolerance. Hop components (lupulones) that adhere to yeast during the brewing process elicit a variety of biological effects including the induction of hepatic cytochrome P4503A. Colupulone was tested for its effects on glucose tolerance and cytochrome P450. Serum glucose levels 30 min after the injection of glucose were lowered by colupulone in nondiabetic Swiss-Webster mice, elevated in diabetic C57B1/KSJ-db/db mice, and unaffected in nondiabetic C57B1/KSJ+m/+m mice. Colupulone lowered hemoglobin glycation slightly in +m/+m mice but not in db/db mice. The cytochrome P450 system was highly induced by colupulone in both db/db and +m/+m mice. Chromium, which acts in concert with the factor in yeast that enhances glucose tolerance, had little or no effect on the plasma glucose level or the cytochrome P450 system in either +m/+m or db/db mice.

  3. An upper-branch Brewer-Dobson circulation index for attribution of stratospheric variability and improved ozone and temperature trend analysis

    Science.gov (United States)

    Ball, William T.; Kuchař, Aleš; Rozanov, Eugene V.; Staehelin, Johannes; Tummon, Fiona; Smith, Anne K.; Sukhodolov, Timofei; Stenke, Andrea; Revell, Laura; Coulon, Ancelin; Schmutz, Werner; Peter, Thomas

    2016-12-01

    We find that wintertime temperature anomalies near 4 hPa and 50° N/S are related, through dynamics, to anomalies in ozone and temperature, particularly in the tropical stratosphere but also throughout the upper stratosphere and mesosphere. These mid-latitude anomalies occur on timescales of up to a month, and are related to changes in wave forcing. A change in the meridional Brewer-Dobson circulation extends from the middle stratosphere into the mesosphere and forms a temperature-change quadrupole from Equator to pole. We develop a dynamical index based on detrended, deseasonalised mid-latitude temperature. When employed in multiple linear regression, this index can account for up to 60 % of the total variability of temperature, peaking at ˜ 5 hPa and dropping to 0 at ˜ 50 and ˜ 0.5 hPa, respectively, and increasing again into the mesosphere. Ozone similarly sees up to an additional 50 % of variability accounted for, with a slightly higher maximum and strong altitude dependence, with zero improvement found at 10 hPa. Further, the uncertainty on all equatorial multiple-linear regression coefficients can be reduced by up to 35 and 20 % in temperature and ozone, respectively, and so this index is an important tool for quantifying current and future ozone recovery.

  4. Control of insects and mites in grain using a high temperature/short time (HTST) technique.

    Science.gov (United States)

    Mourier; Poulsen

    2000-07-01

    Wheat infested with grain mites (Acari) and Sitophilus granarius, and maize infested with Prostephanus truncatus, were exposed to hot air in a CIMBRIA HTST Microline toaster((R)). Inlet temperatures of the hot air were in the range of 150-750 degrees C decreasing to outlet temperatures in the range of 100-300 degrees C during the exposure period. A rotating drum, connected to a natural-gas burner was fed with grain which was in constant movement along the drum and thereby mixed thoroughly during the process. The capacity of the toaster was 1000 kg per hour.Complete control of grain mites and adult S. granarius in wheat was obtained with an inlet temperature of 300-350 degrees C and an average residence time in the drum of 6 s. More than 99% mortality was obtained for all stages of S. granarius with an inlet temperature of 300-350 degrees C and an average exposure period of 40 s. For control of P. truncatus in maize, an inlet temperature of 700 degrees C resulted in a complete disinfestation when the exposure time was 19 s.The reduction in grain moisture content was 0.5-1% at treatments giving 100% control. Germination tests indicate that it is possible to choose a combination of inlet temperatures and exposure periods which effectively kills mites and insects in small grains, without harming the functional properties of the grain.Economy of the method was considered to be competitive with fumigation using phosphine.

  5. Performance of broiler fed pure glycerine

    Directory of Open Access Journals (Sweden)

    Dássia Daiane Oliveira

    2014-02-01

    Full Text Available Two experiments evaluated the pure glycerin in broiler chicken diets. Experiment 1 was a metabolism test using total feces sampling method with 96 male chickens aging from 17 to 25 d when animals were fed on two treatments: diet1 = no glycerin and diet2 = 60g/kg of glycerin. The apparent metabolized energy measured 4015 kcal/kg and the apparent metabolized corrected for nitrogen balance was 3911 kcal/kg. Experiment 2 evaluated weight gains, feed intake and feed conversion in 480 chicks at 6, 20 and 34 d old fed on diets with 0, 40, 80 and 120 g/kg of glycerin. The results indicate that pure glycerin in chicken diets, as a source of energy must take into consideration the age of the animals and it may be added up to 120 g/kg, from 20 to 41 d of age.

  6. Qualitative characteristics of meat from confined crossbred heifers fed with lipid sources

    Directory of Open Access Journals (Sweden)

    Giovani Fiorentini

    2012-10-01

    Full Text Available Lipids have been used in ruminant feed to replace high amounts of grain for increasing the diet energy density, performance and meat quality. This study evaluated the qualitative characteristics of meat from feedlot heifers fed with sources of lipid supplements. Twenty-one crossbred heifers (1/4Nelore × 1/4Santa Gertrudis × 1/2Braunvieh were used. Each heifer received 60 % forage with a base of corn silage and 40 % concentrate, resulting in 5.8 % lipid content in the total diet. The following sources of lipids were used: soybeans, protected fat and soybean oil. There were no differences on physical characteristics of meat samples from heifers fed with the lipid sources. Soybeans increased the concentration of linoleic acid, content of polyunsaturated fatty acid and activity of the Δ9-desaturase C16 enzyme in the Longissimus muscle. The use of soybean oil in the diet increased the oleic acid, monounsaturated fatty acid, total cis- and trans-fatty acids (C18:0 and the activity of the Δ9-desaturase C16 enzyme in the subcutaneous fat. Diets with soybean grain had greater deposition of linoleic and linolenic acids than diets with fat protected and greater presence of these essential fatty acids are associated to a better composition and meat quality.

  7. Spinodal decomposition in fine grained materials

    Indian Academy of Sciences (India)

    H Ramanarayan; T A Abinandanan

    2003-01-01

    We have used a phase field model to study spinodal decomposition in polycrystalline materials in which the grain size is of the same order of magnitude as the characteristic decomposition wavelength ($\\lambda_{SD}$). In the spirit of phase field models, each grain () in our model has an order parameter ($\\eta_i$) associated with it; $\\eta_i$ has a value of unity inside the th grain, decreases smoothly through the grain boundary region to zero outside the grain. For a symmetric alloy of composition, = 0.5, our results show that microstructural evolution depends largely on the difference in the grain boundary energies, $\\gamma_{gb}$, of A-rich () and B-rich () phases. If $\\gamma^{\\alpha}_{gb}$ is lower, we find that the decomposition process is initiated with an layer being formed at the grain boundary. If the grain size is sufficiently small (about the same as $\\lambda_{SD}$), the interior of the grain is filled with the phase. If the grain size is large (say, about 10 $\\lambda_{SD}$ or greater), the early stage microstructure exhibits an A-rich grain boundary layer followed by a B-rich layer; the grain interior exhibits a spinodally decomposed microstructure, evolving slowly. Further, grain growth is suppressed completely during the decomposition process.

  8. A Study of End-Fed Arrays

    CERN Document Server

    Kondylis, Konstantinos; Zimourtopoulos, Petros

    2010-01-01

    This paper presents a general analysis of end-fed space arrays with application to self-standing linear arrays of parallel dipoles. A number of test array models were simulated, constructed and their radiation pattern was then measured. The experimental and computational results were found to be in good agreement. The developed software applications are available through the Internet as FLOSS Free Libre Open Source Software.

  9. Harmonic analysis of Doubly Fed Induction Generators

    DEFF Research Database (Denmark)

    Lindholm, Morten; Rasmussen, Tonny Wederberg

    2003-01-01

    This paper gives an overview of the frequency spectrum of the stator and rotor currents in a doubly fed induction generator (DFIG) used in wind power applications. The paper also presents a method to eliminate higher harmonics and interharmonics in the DFIG stator current. The method is implemented...... on a 40 kW laboratory model connected to the utility-grid, where the DFIG is supplied by a back-to-back 3-level NPC-converter....

  10. Phenomenology of Abnormal Grain Growth in Systems with Nonuniform Grain Boundary Mobility

    Science.gov (United States)

    DeCost, Brian L.; Holm, Elizabeth A.

    2017-06-01

    We have investigated the potential for nonuniform grain boundary mobility to act as a persistence mechanism for abnormal grain growth (AGG) using Monte Carlo Potts model simulations. The model system consists of a single initially large candidate grain embedded in a matrix of equiaxed grains, corresponding to the abnormal growth regime before impingement occurs. We assign a mobility advantage to grain boundaries between the candidate grain and a randomly selected subset of the matrix grains. We observe AGG in systems with physically reasonable fractions of fast boundaries; the probability of abnormal growth increases as the density of fast boundaries increases. This abnormal growth occurs by a series of fast, localized growth events that counteract the tendency of abnormally large grains to grow more slowly than the surrounding matrix grains. Resulting abnormal grains are morphologically similar to experimentally observed abnormal grains.

  11. Molecule Formation on Interstellar Grains

    Science.gov (United States)

    Vidali, G.

    2011-05-01

    The first experiments that were expressively designed to be applicable to hydrogen formation reactions in the ISM measured the efficiency of formation of molecular hydrogen on a polycrystalline olivine (Pirronello et al. (1997a)). It soon turned out that more was needed, and research began on the mechanism of reaction, on the in uence of the surface morphology, and on the excitation of the just- ormed molecule. In this review, I summarize what we learned from these and other experiments, and where more work is needed: in the elementary steps of reaction, in the bridging of the laboratory-ISM gap (large ux/large surface - small ux/small grain) using simulations, and in using realistic samples of dust grains. Understanding what experiments can and cannot deliver will help in designing and targeting observations, and vice-versa.

  12. Coarse-graining complex dynamics

    DEFF Research Database (Denmark)

    Sibani, Paolo

    2013-01-01

    Continuous Time Random Walks (CTRW) are widely used to coarse-grain the evolution of systems jumping from a metastable sub-set of their configuration space, or trap, to another via rare intermittent events. The multi-scaled behavior typical of complex dynamics is provided by a fat-tailed distribu......Continuous Time Random Walks (CTRW) are widely used to coarse-grain the evolution of systems jumping from a metastable sub-set of their configuration space, or trap, to another via rare intermittent events. The multi-scaled behavior typical of complex dynamics is provided by a fat......-law and logarithmic relaxation behaviors ubiquitous in complex dynamics, together with the sub-diffusive time dependence of the Mean Square Displacement characteristic of single particles moving in a complex environment....

  13. Efficient radiative transfer in dust grain mixtures

    CERN Document Server

    Wolf, S

    2003-01-01

    The influence of a dust grain mixture consisting of spherical dust grains with different radii and/or chemical composition on the resulting temperature structure and spectral energy distribution of a circumstellar shell is investigated. The comparison with the results based on an approximation of dust grain parameters representing the mean optical properties of the corresponding dust grain mixture reveal that (1) the temperature dispersion of a real dust grain mixture decreases substantially with increasing optical depth, converging towards the temperature distribution resulting from the approximation of mean dust grain parameters, and (2) the resulting spectral energy distributions do not differ by more than 10% if >= 2^5 grain sizes are considered which justifies the mean parameter approximation and the many results obtained under its assumption so far. Nevertheless, the dust grain temperature dispersion at the inner boundary of a dust shell may amount to >>100K and has therefore to be considered in the cor...

  14. Solid Propellant Grain Structural Integrity Analysis

    Science.gov (United States)

    1973-01-01

    The structural properties of solid propellant rocket grains were studied to determine the propellant resistance to stresses. Grain geometry, thermal properties, mechanical properties, and failure modes are discussed along with design criteria and recommended practices.

  15. Grain legumes and human health

    OpenAIRE

    Lambein, Fernand; Kuo, Yu-Haey; Ikegami, Fumio; Kusama-Eguchi, Kuniko; Enneking, Dirk

    2009-01-01

    Since early civilisation in different continents, grain legumes have been a part of balanced diets together with cereals. In general the seeds from those two plant families give together a good source of essential amino acids and may have given an evolutionary advantage. A sustainable diet for vegetarians may not be possible without the protein-rich legumes. Consumed alone, legumes can be a mixed blessing because of their deficiency of some essential amino acids. This deficiency can be balanc...

  16. Automatic Coarse Graining of Polymers

    OpenAIRE

    Faller, Roland

    2003-01-01

    Several recently proposed semi--automatic and fully--automatic coarse--graining schemes for polymer simulations are discussed. All these techniques derive effective potentials for multi--atom units or super--atoms from atomistic simulations. These include techniques relying on single chain simulations in vacuum and self--consistent optimizations from the melt like the simplex method and the inverted Boltzmann method. The focus is on matching the polymer structure on different scales. Several ...

  17. Grain boundary wetness of partially molten dunite

    Science.gov (United States)

    Mu, S.; Faul, U.

    2013-12-01

    The grain scale melt distribution plays a key role for physical properties of partially molten regions in Earth's upper mantle, but our current understanding of the distribution of basaltic melt at the grain scale is still incomplete. A recent experimental study shows that wetted two-grain boundaries are a common feature of partially molten dunite at small melt fractions (Garapic et al., G3, 2013). In early ideal models which assume isotropic surface energy, the grain scale melt distribution is uniquely determined by knowing the melt fraction and the dihedral angle between two crystalline grains and the melt (von Bargen and Waff, JGR, 1986). Olivine is anisotropic in surface energy, hence the grain scale melt distribution at given melt fraction cannot be characterized by the dihedral angle alone. The grain boundary wetness, which is defined as the ratio of solid-liquid boundary area over the total interfacial area (Takei, JGR, 1998), is a more objective measure of the grain scale melt distribution. The aim of this study is to quantify the relationship between grain size, melt fraction, temperature and grain boundary wetness of partially molten dunite under dry conditions. We annealed olivine-basalt aggregates with melt fractions from 0.03% to 6% at a range of temperatures and 1 GPa in a piston cylinder for 1 to 336 hours, with resulting mean grain sizes of 10 to 60 μm. The samples were sectioned, polished and imaged at high resolution by using a field emission SEM. Each image had a size of 2048 x 1536 pixels with a resolution of 0.014 to 0.029 μm/pixel, depending on magnification. For each sample, depending on grain sizes, we made mosaics of 3 x 3 or 6 x 6 overlapping images. Measurements of melt fraction, grain boundary wetness and grain size were carried out on these high resolution mosaics by using ImageJ software. Analyses of mosaics show that grain boundary wetness increases with increasing melt fraction at constant grain size to values well above those

  18. Grain Boundary Energies in Copper.

    Science.gov (United States)

    Omar, Ramli

    Available from UMI in association with The British Library. Requires signed TDF. The dependence of grain boundary energy on boundary orientation was studied in copper annealed at 1000 ^circC. Grain boundary orientations and the disorientations across the boundaries were measured. A rotation matrix notation is used to interpret selected area electron channelling patterns observed in a scanning electron microscope. The Herring and Shewmon torque terms were investigated using wire specimens having a "bamboo" structure. The Herring torque terms were determined using the Hess relation. The (110) section of the Sigma 11 gamma-plot (i.e. the variation of grain boundary energy with boundary orientation) was evaluated. In this plot, minima in energies were found at the (311) and (332) mirror planes. Sigma 3 and Sigma9 boundaries were investigated in sheet specimens. The (110) and (111) sections of the Sigma3 gamma -plot were evaluated. In addition to the sharp cusps occurring at the Sigma3 {111} planes, the further shallower cusps occur at the incoherent Sigma 3 boundaries with the interfacial planes approximately parallel to {322} in one crystal and {11.44} in the other crystal. Flat and curved Sigma9 boundaries were investigated. The break up of Sigma9 boundaries into two Sigma3 boundaries and the relation between the Sigma3 and Sigma 9 gamma-plots was also examined. The (110) section of the Sigma9 gamma-plot was constructed.

  19. Whole grains: benefits and challenges.

    Science.gov (United States)

    Jones, Julie Miller; Engleson, Jodi

    2010-01-01

    Inclusion of whole grains (WG) in the diet is recommended in dietary guidance around the world because of their associations with increased health and reduced risk of chronic disease. WGs are linked to reduced risk of obesity or weight gain; reduced risk of cardiovascular disease (CVD), including coronary heart disease (CHD), hypertension, and stroke; improved gut health and decreased risk of cancers of the upper gut; perhaps reduced risk of colorectal cancer; and lower mortality rate. The 2005 United States Dietary Guidelines Advisory Committee has recommended that consumers make "half their grains whole." Yet, whole grains are puzzling both consumers and scientists. Scientists are trying to determine whether their health benefits are due to the synergy of WG components, individual WG components, or the fact that WG eaters make many of the recommended diet and lifestyle choices. Consumers need to understand the WG benefits and how to identify WG foods to have incentive to purchase and use such foods. Industry needs to develop great-tasting, clearly-labeled products. With both these factors working together, it will be possible to change WG consumption habits among consumers.

  20. Evaluation of a fine sediment removal tool in spring-fed and snowmelt driven streams

    Science.gov (United States)

    Sepulveda, Adam; Layhee, Megan J.; Sutphin, Zach; Sechrist, Juddson D.

    2015-01-01

    The accumulation of fine-grained sediments impairs the structure and function of streams, so removing fine sediments may be required to achieve restoration objectives. There has been little work on methods of removing excess sediment or on the efficacy of the methods. We used a 4-year before-after-control-impact design in southeastern Idaho streams to test a fine sediment removal system (FSRS) manufactured by Streamside Environmental LLC. The FSRS agitates fine sediment in the substrate with clean pump water and then vacuums the sediment out of the stream with a second pump. Our objectives were: 1) to test if the FSRS can selectively remove fine sediment; 2) to monitor the bio-physical responses in FSRS treated and downstream waters; and 3) to compare the bio-physical responses to the FSRS in spring-fed and snowmelt driven stream reaches. The FSRS removed ~ 14 metric tons of sediment from the two treated reaches. More than 90% of this sediment was < 2 mm, indicating that the FSRS selected for fine sediment in both stream types. Sustained effects of removing this sediment were confined to substrate improvements in treated reaches. Embeddedness in the spring-fed reach decreased and subsurface grain size in spring-fed and snowmelt driven reaches increased. We did not detect any sustained invertebrate or fish responses in treated reaches or any detrimental bio-physical responses in downstream waters. These results indicate that the FSRS reduced fine sediment levels but sediment removal did not reverse the impacts of sediment accumulation to stream biota within our monitoring time frame.

  1. Grain Boundary (GB) Studies in Nano- and Micro- Crystalline Materials

    OpenAIRE

    Tanju, Mst Sohanazaman

    2011-01-01

    Polycrystalline materials are composed of grains and grain boundaries. The total volume of occupied grain boundaries in polycrystalline material depends on the grain size. When grain size decreases the volume fraction of grain boundaries increases. For example, when grain size is 10 nm grain boundary volume fraction is ~ 25%. In polycrystalline materials, different properties (mechanical, electrical, optical, magnetic) are affected by the size of their grains and by the atomic structure of...

  2. GRAIN YIELD STABILITY OF WINTER OAT (AVENA SATIVA L. CULTIVARS IN THE CENTRAL ANATOLIAN REGION OF TURKEY

    Directory of Open Access Journals (Sweden)

    Mevlut AKCURA

    2006-05-01

    Full Text Available The objectives of this research were to assess genotype environment interaction and determine stable oat (Avena sativa L. cultivars for grain yield in Central Anatolian Region of Turkey. Stability analysis [9] were to performed on results for grain yield of 5 oat cultivars (Chekota, Yesilkoy-1779, Yesilkoy-330, Faikbey-2004, Seydisehir-2004 from 24 trials (6 irrigated, 18 rain fed was conducted over 6 years in the Central Anatolian Region, Turkey. There was considerable variation in grain yield within and across environments. Year by location and location variability were dominant sources of interactions. The cultivar, “Seydisehir-2004” with respective regression coefficient value of 1.03, the smallest deviations from regressions (S2di value and the highest grain yield could be considered the most widely adapted cultivar. The other test cultivars were sensitive to production-limiting factors, their wider adaptability, stability and general performance to the fluctuating growing conditions within and across environments being lowered.

  3. Determination of grain boundary geometry using TEM

    NARCIS (Netherlands)

    Jang, H.; Farkas, D.; Hosson, J.T.M. De

    1992-01-01

    An experimental method to obtain the grain boundary geometry using the transmission electron microscope is presented. The method allows Σ determination including grain boundary plane orientation. In order to determine the specialness of the grain boundary, three different criteria for maximum allowa

  4. Bioactive compounds in whole grain wheat

    NARCIS (Netherlands)

    Mateo Anson, N.

    2010-01-01

    Bread can be healthier! Consuming whole-grain foods can prevent cardiovascular diseases, type-2 diabetes and metabolic syndrome. This is due to bioactive compounds in whole grain, such as antioxidants and anti-inflammatory compounds. We found that the different fractions of a wheat grain vary much

  5. Determination of grain boundary geometry using TEM

    NARCIS (Netherlands)

    Jang, H.; Farkas, D.; Hosson, J.T.M. De

    An experimental method to obtain the grain boundary geometry using the transmission electron microscope is presented. The method allows Σ determination including grain boundary plane orientation. In order to determine the specialness of the grain boundary, three different criteria for maximum

  6. Red grain mycetoma foot in Western Rajasthan

    Directory of Open Access Journals (Sweden)

    Mathur D

    1994-01-01

    Full Text Available Usually the colour of the grains seen in cases of mycetoma are either black or yellow. Recently there were reports that unusual red grains had been noticed in cases of mycetoma. A case of red grain mycetoma is reported.

  7. Bioactive compounds in whole grain wheat

    NARCIS (Netherlands)

    Mateo Anson, N.

    2010-01-01

    Bread can be healthier! Consuming whole-grain foods can prevent cardiovascular diseases, type-2 diabetes and metabolic syndrome. This is due to bioactive compounds in whole grain, such as antioxidants and anti-inflammatory compounds. We found that the different fractions of a wheat grain vary much i

  8. Bioactive compounds in whole grain wheat

    NARCIS (Netherlands)

    Mateo Anson, N.

    2010-01-01

    Bread can be healthier! Consuming whole-grain foods can prevent cardiovascular diseases, type-2 diabetes and metabolic syndrome. This is due to bioactive compounds in whole grain, such as antioxidants and anti-inflammatory compounds. We found that the different fractions of a wheat grain vary much i

  9. Structure and chemistry of the sorghum grain

    Science.gov (United States)

    Sorghum is grown around the world and often under harsh and variable environmental conditions. Combined with the high degree of genetic diversity present in sorghum, this can result in substantial variability in grain composition and grain quality. While similar to other cereal grains such as maize ...

  10. Whole grain gluten-free flat breads

    Science.gov (United States)

    The USDA food guide recommends that at least ½ of all the grains eaten should be whole grains. The FDA allows food health claim labels for food containing 51% whole gains and 11 g of dietary fiber. This is the only report demonstrating innovative whole grain gluten free (without yeast or chemicals) ...

  11. Ancient whole grain gluten-free flatbreads

    Science.gov (United States)

    The USDA food guide recommends that at least ½ of all the grains eaten should be whole grains. The FDA allows food Health Claim labels for food containing 51% whole gains and 11 g of dietary fiber. This is the only report demonstrating innovative ancient whole grain gluten-free (no yeast or chemical...

  12. 77 FR 74781 - Safety Zones; Columbia Grain and United Grain Corporation Facilities; Columbia and Willamette Rivers

    Science.gov (United States)

    2012-12-18

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zones; Columbia Grain and United Grain.... SUMMARY: The Coast Guard is establishing temporary safety zones around the Columbia Grain facility on the Willamette River in Portland, OR, and the United Grain Corporation facility on the Columbia River in...

  13. 75 FR 76254 - Official Performance and Procedural Requirements for Grain Weighing Equipment and Related Grain...

    Science.gov (United States)

    2010-12-08

    ... Grain Inspection, Packers and Stockyards Administration 7 CFR Part 802 RIN 0580-AB19 Official Performance and Procedural Requirements for Grain Weighing Equipment and Related Grain Handling Systems AGENCY: Grain Inspection, Packers and Stockyards Administration, USDA. ACTION: Direct final rule. SUMMARY: The...

  14. Grain-size sorting and slope failure in experimental subaqueous grain flows

    NARCIS (Netherlands)

    Kleinhans, M.G.; Asch, Th.W.J. van

    2005-01-01

    Grain-size sorting in subaqueous grain flows of a continuous range of grain sizes is studied experimentally with three mixtures. The observed pattern is a combination of stratification and gradual segregation. The stratification is caused by kinematic sieving in the grain flow. The segregation is

  15. Weighing in on whole grains: A review of evidence linking whole grains to body weight

    Science.gov (United States)

    U.S. dietary guidelines support the consumption of whole grains in lieu of refined grains. On January 31, 2011, the 2010 Dietary Guidelines for Americans (DGA) were released and the recommendations with respect to grains were for individuals to “Consume at least half of all grains as whole grains” a...

  16. Choosing Whole-Grain Foods: 10 Tips for Purchasing and Storing Whole-Grain Foods

    Science.gov (United States)

    ... Newsroom Dietary Guidelines Communicator’s Guide 10 Tips: Choosing Whole-Grain Foods You are here Home 10 Tips: Choosing Whole-Grain Foods Print Share 10 Tips: Choosing Whole-Grain Foods Whole grains are important sources of nutrients ...

  17. Grain-size sorting and slope failure in experimental subaqueous grain flows

    NARCIS (Netherlands)

    Kleinhans, M.G.; Asch, Th.W.J. van

    2005-01-01

    Grain-size sorting in subaqueous grain flows of a continuous range of grain sizes is studied experimentally with three mixtures. The observed pattern is a combination of stratification and gradual segregation. The stratification is caused by kinematic sieving in the grain flow. The segregation is ca

  18. 啤酒糟发酵产高蛋白饲料菌种的的配伍%Compatibility of strains for producing higher protein feed by fermentation of brewer's residue

    Institute of Scientific and Technical Information of China (English)

    陶敏; 王维嘉; 蔡俊

    2011-01-01

    以啤酒糟为主要原料,采用多菌种混合固态发酵生产蛋白饲料,并通过菌种的配伍实验对发酵产蛋白饲料菌种的组合进行优化,试验结果表明:挑选的8株菌都能在以啤酒糟为唯一底物的平板上生长,并且菌种最佳组合为固氮菌、白地霉、绿色木霉、啤酒酵母、热带假丝酵母等5株菌,其发酵后的蛋白质含量为31.45%,粗蛋白为39.22%,无机氮转化率为56.42%.%With brewer's residue as main materials, through solid-state fermentation to produce protein feed by multiplex strains, and optimizing the strain compatibility experiment of producing protein feed by fermentation optimized combination.Experimental results showed that the selected eight strains were growing on the only substrate of brewer's residue, and the best combination of strains were Azotobacter,geotrichum candidum, trichoderma viride, beer yeast, candida tropicalis etc, after fermentation of brewer's residue, the protein content was 31.45%, crude protein was 39.22%, conversion of inorganic nitrogen was 56.42%.

  19. Subsonic Mechanical Alignment of Irregular Grains

    CERN Document Server

    Lazarian, Alex

    2007-01-01

    We show that grains can be efficiently aligned by interacting with a subsonic gaseous flow. The alignment arises from grains having irregularities that scatter atoms with different efficiency in the right and left directions. The grains tend to align with long axes perpendicular to magnetic field, which corresponds to Davis-Greenstein predictions, but does not involve magnetic field. For rather conservative factors characterizing the grain helicity and scattering efficiency of impinging atoms, the alignment of helical grains is much more efficient than the Gold-type alignment processes.

  20. Spinodal decomposition in fine grained materials

    OpenAIRE

    Ramanarayan, H.; Abinandanan, TA

    2003-01-01

    We have used a phase field model to study spinodal decomposition in polycrystalline materials in which the grain size is of the same order of magnitude as the characteristic decomposition wavelength (lambda(SD))In the spirit of phase field models, each grain (i) in our model has an order parameter (eta(i)) associated with it; eta(i) has a value of unity inside the ith grain, decreasessmoothly through the grain boundary region to zero outside the grain. For a symmetric alloy of composition, c ...

  1. 3D modeling of metallic grain growth

    Energy Technology Data Exchange (ETDEWEB)

    George, D.; Carlson, N.; Gammel, J.T.; Kuprat, A.

    1999-06-01

    This paper will describe simulating metallic grain growth using the Gradient Weighted Moving Finite Elements code, GRAIN3D. The authors also describe the set of mesh topology change operations developed to respond to changes in the physical topology such as the collapse of grains and to maintain uniform calculational mesh quality. Validation of the method is demonstrated by comparison to analytic calculations. The authors present results of multigrain simulations where grain boundaries evolve by mean curvature motion and include results which incorporate grain boundary orientation dependence.

  2. Airborne Pollen Grains Of Afyon, Turkey

    Institute of Scientific and Technical Information of China (English)

    Adem BICAKCI; Süheyla ERGUN; Sevcan TATLIDIL; Hulusi MALYER; Sabri OZYURT; Ahmet AKKAYA; Nihat SAPAN

    2002-01-01

    The airborne pollen grains of Afyon have been studied for a two-year period (1999-2000) with a Durham sampler. A total of 14 367 pollen grains belonging to 40 taxa have been identified and recorded with some unidentified ones. Of them, 6 732 were identified in 1999 and 7 635 in 2000. Of the total pollen grains, 69.67% were arboreal, 26.64% non-arboreal and 3.68 % unidentified. The majority of the investigated pollen grains were from Pinus, Gramineae, Cupressaceae, Platanus, Chenopodiaceae/Amaranthaceae, Quercus, Ailanthus, Moraceae, Juglans, Salix, Cedrus and Rosaceae. The highest level of pollen grains was in May.

  3. Role of cysteines in the activation and inactivation of brewers' yeast pyruvate decarboxylase investigated with a PDC1-PDC6 fusion protein.

    Science.gov (United States)

    Zeng, X; Farrenkopf, B; Hohmann, S; Dyda, F; Furey, W; Jordan, F

    1993-03-16

    Possible roles of the Cys side chains in the activation and inactivation mechanisms of brewers' yeast pyruvate decarboxylase were investigated by comparing the behavior of the tetrameric enzyme pdc1 containing four cysteines/subunit (positions 69, 152, 221, and 222) with that of a fusion enzyme (pdc1-6, a result of spontaneous gene fusion between PDC1 and PDC6 genes) that is 84% identical in sequence with pdc1 and has only Cys221 (the other three Cys being replaced by aliphatic side chains). The two forms of the enzyme are rather similar so far as steady-state kinetic parameters and substrate activation are considered, as tested for activation by the substrate surrogate pyruvamide. Therefore, if a cysteine is responsible for substrate activation, it must be Cys221. The inactivation of the two enzymes was tested with several inhibitors. Methylmethanethiol sulfonate, a broad spectrum sulfhydryl reagent, could substantially inactivate both enzymes, but was slightly less effective toward the fusion enzyme. (p-Nitrobenzoyl)formic acid is an excellent alternate substrate, whose decarboxylation product p-nitrobenzaldehyde inhibited both enzymes possibly at a Cys221, the only one still present in the fusion enzyme. Exposure of the fusion enzyme, just as of pdc1, to (E)-2-oxo-4-phenyl-3-butenoic acid type inhibitors/alternate substrates enabled detection of the enzyme-bound enamine intermediate at 440 nm. However, unlike pdc1, the fusion enzyme was not irreversibly inactivated by these substrates. These substrates are now known to cause inactivation of pdc1 with concomitant modification of one Cys of the four [Zeng, X.; Chung, A.; Haran, M.; Jordan, F. (1991) J. Am. Chem. Soc. 113, 5842-49].(ABSTRACT TRUNCATED AT 250 WORDS)

  4. Preparation of Protein Hydrolysates with Waste Brewer's Yeast%啤酒废酵母蛋白水解物的制取工艺

    Institute of Scientific and Technical Information of China (English)

    张晓鸣; 袁信华; 徐柔; 章克昌

    2001-01-01

    Protein hydrolysates were prepared by enzymatic hydrolysis ofpretreated waste brewer's yeast. The hydrolysis parameters were specified by response surface analysis. The Parameters are as follows: initial yeast concentration 15%, temperature 55~60 ℃, the β-glucanase-substrate ratio 15~20 U/g, and pH 6.6~6.8. When hydrolysed for 7 hour, more than 82% of the Kjeldahl protein in yeast was extracted. Measured by high-performance liquid chromatography(HPLC), 65.26% of the hydrolysates had molecular weights of 210~635 Dalton, which corresponded to dipeptides and tripeptides.%利用啤酒废酵母资源,通过破壁预处理与复合酶降解相结合的工艺,制取特定相对分子质量范围的蛋白水解物.响应面分析结果表明,在温度55~60℃,pH6.6~6.8,β-葡聚糖酶用量15~20U/g(以酵母计)的条件下,有最高的肽提取率.酶解7h,蛋白质提取率大于82%,经HPLC测定,水解产物中65%组分的相对分子质量在210~635之间,处于二肽和三肽相对分子质量范围.

  5. The upward branch of the Brewer-Dobson circulation quantified by tropical stratospheric water vapor and carbon monoxide measurements from the Aura Microwave Limb Sounder

    Science.gov (United States)

    Minschwaner, K.; Su, H.; Jiang, J. H.

    2016-03-01

    The vertical distributions of water vapor (H2O) and carbon monoxide (CO) in the tropical lower stratosphere are controlled largely by their mixing ratios near the tropopause and by ascending motions as part of the Brewer-Dobson circulation (BDC). The upward propagation of seasonal variations imprinted on H2O and CO vertical profiles, often referred to as the tropical "tape recorder," can be used to derive the mean vertical velocity, w*>¯, in this region of the lower stratosphere where quasi-horizontal mixing is not strong enough to erase the seasonal tape recorder signals. We used Aura Microwave Limb Sounder observations of the tropical tape recorders from 2004 to 2014 to derive values of w*>¯ at pressures between 90 and 16 hPa (about 18 to 28 km altitude). Mean vertical profiles of w*>¯ are consistent with calculated velocities derived from net radiative heating rates based on observed temperature, humidity, cloud, and trace gas amounts. Temporal variations in w*>¯ are dominated by a quasi-biennial oscillation (QBO) and seasonal cycles, with maximum upwelling coinciding with easterly phases of the QBO in zonal wind shear and during the November-December period of the seasonal cycle. Both the QBO and annual modes emphasize the importance of wave phenomena in modulating the strength of tropical upwelling in the BDC. Interannual anomalies in w*>¯ are correlated with variations in the El Niño-Southern Oscillation (ENSO), with enhanced stratospheric upwelling during El Niño phases and reduced upwelling during La Niña. A small decreasing linear trend (~6%/decade) in w*>¯ is observed from 2005 to 2014, although confidence is low in identifying such a trend as part of a long-term change due to the influence of ENSO over this period.

  6. High Precision, Absolute Total Column Ozone Measurements from the Pandora Spectrometer System: Comparisons with Data from a Brewer Double Monochromator and Aura OMI

    Science.gov (United States)

    Tzortziou, Maria A.; Herman, Jay R.; Cede, Alexander; Abuhassan, Nader

    2012-01-01

    We present new, high precision, high temporal resolution measurements of total column ozone (TCO) amounts derived from ground-based direct-sun irradiance measurements using our recently deployed Pandora single-grating spectrometers. Pandora's small size and portability allow deployment at multiple sites within an urban air-shed and development of a ground-based monitoring network for studying small-scale atmospheric dynamics, spatial heterogeneities in trace gas distribution, local pollution conditions, photochemical processes and interdependencies of ozone and its major precursors. Results are shown for four mid- to high-latitude sites where different Pandora instruments were used. Comparisons with a well calibrated double-grating Brewer spectrometer over a period of more than a year in Greenbelt MD showed excellent agreement and a small bias of approximately 2 DU (or, 0.6%). This was constant with slant column ozone amount over the full range of observed solar zenith angles (15-80), indicating adequate Pandora stray light correction. A small (1-2%) seasonal difference was found, consistent with sensitivity studies showing that the Pandora spectral fitting TCO retrieval has a temperature dependence of 1% per 3K, with an underestimation in temperature (e.g., during summer) resulting in an underestimation of TCO. Pandora agreed well with Aura-OMI (Ozone Measuring Instrument) satellite data, with average residuals of <1% at the different sites when the OMI view was within 50 km from the Pandora location and OMI-measured cloud fraction was <0.2. The frequent and continuous measurements by Pandora revealed significant short-term (hourly) temporal changes in TCO, not possible to capture by sun-synchronous satellites, such as OMI, alone.

  7. Establishment of Grain Farmers’ Supply Response Model and Empirical Analysis under Minimum Grain Purchase Price Policy

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Based on farmers’ supply behavior theory and price expectations theory,this paper establishes grain farmers’ supply response model of two major grain varieties (early indica rice and mixed wheat) in the major producing areas,to test whether the minimum grain purchase price policy can have price-oriented effect on grain production and supply in the major producing areas. Empirical analysis shows that the minimum purchase price published annually by the government has significant positive impact on farmers’ grain supply in the major grain producing areas. In recent years,China steadily raises the level of minimum grain purchase price,which has played an important role in effectively protecting grain farmers’ interests,mobilizing the enthusiasm of farmers’ grain production,and ensuring the market supply of key grain varieties.

  8. Faecal Excretion of Glycosphingolipids of Breast-fed and Formula-fed Infants

    OpenAIRE

    Larson, G.; Falk, P; Hynsjö, L.; Midtvedt, A. -C.; Midtvedt, T

    2011-01-01

    Faecal samples of six healthy breast-fed, or formula-fed, infants were collected at intervals up to 9 mth of age and analysed for their contents of sphingolipids. Comparisons were made with faecal samples of one older child and one adult. The mean faccal content of sphingolipids during the first 9 mth of life (8.2 μmol sphingosine/g dry stool) was significantly less than that of meconium samples (23.3 μmol/gj but higher than that of adult stools (1.2 μmol/g). Sphingomyelin excretion ...

  9. Evaluation of feeding distiller's grains, containing virginiamycin, on antimicrobial susceptibilities in fecal isolates of Enterococcus and Escherichia coli and prevalence of resistance genes in cattle

    Science.gov (United States)

    Dried distiller’s grains (DG), produced from fermentations using no antibiotic (Control) or dosed with 2 or 20 ppm virginiamycin product and containing 0, 0.7, and 8.9 ppm virginiamycin, respectively, were fed to cattle and effects on antibiotic sensitivity and prevalence of resistance genes in comm...

  10. Effects of increasing inclusion rates of a low-fat distillers dried grains with solubles (LF-DDGS) in finishing broiler diets

    Science.gov (United States)

    The biodiesel industry is now removing corn oil from distillers dried grains with solubles (DDGS) in order to meet increasing demand. The objectives of this study were to determine the maximum inclusion rates in broiler diets fed from 28 to 42 d of age in the finishing phases of production and the ...

  11. Effects of sorghum wet distillers grains plus solubles in steam-flaked corn-based finishing diets on steer performance, carcass characteristics, and digestibility characteristics

    Science.gov (United States)

    Two studies were conducted to evaluate the effects of sorghum wet distillers grains (SWDGS) in finishing diets on steer performance, carcass characteristics, and nutrient digestibility. In Exp. 1, 240 steers (initial BW = 379 +/-1 kg) were fed steam-flaked corn (SFC)-based diets with or without 25%...

  12. Study on Fluctuation of Grain Yield in China’s Major Grain Producing Areas

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    By using the statistical data of grain yield in China’s major grain producing areas from 1949 to 2008,and fluctuation theory,the historical process and main cause of fluctuation of grain yield in China’s major grain producing areas are analyzed.The results of research show that the grain yield in China’s major grain producing areas grows in unstable fluctuation,with high-frequency fluctuation cycle and regular length;the amplitude of fluctuation,on the whole,is moderate,with not strong stability;the fluctuation of grain yield has correspondence,reflecting the N-shape developmental trend of grain production at present;the fluctuation of grain yield has gradient characteristics;in the process of comparison of grain yield,the average growth rate annually of grain yield in China’s major grain producing areas is higher than that of the national average,but the relative fluctuation coefficient is also higher than that of the national average.From five aspects,namely natural disaster,agricultural policy,production input,grain price and grain circulation,the cause of fluctuation of grain yield in China’s major grain producing areas is analyzed,and measures of preventing and arresting super-long fluctuation of grain yield are put forward.Firstly,stick to strict farmland protection system,and strive to promote farmland quality;secondly,strengthen infrastructure construction of grain production and beef up the ability of preventing natural disaster;thirdly,quicken the pace of agricultural technology and establish robust technology supporting system;fourthly,lay stress on innovation of agricultural organization system and provide implementation path and vehicle for application of agricultural technology measures;fifthly,perfect disaster precaution system and grain market system,and strengthen the ability of preventing risk of grain production.

  13. Litter characteristics and pododermatitis incidence in broilers fed a sorghum-based diet

    Directory of Open Access Journals (Sweden)

    CMC Carvalho

    2014-09-01

    Full Text Available The aim of this study was to evaluate the incidence of footpad dermatitis and quality of broilers litter fed with sorghum grain and diets based in corn. It was used 544 male and female chicks, distributed in a completely randomized design with two treatments and eight replications. The chicken feed was formulated and produced from corn, soybean meal and sorghum grain. In the formulation were kept constant levels of energy and protein in accordance with the following treatments: A. Control (diet based on corn and soybean meal; B. Grain sorghum (Whole Sorghum + soybean meal. At 35 and 42 days were evaluated mineral matter (A %, calcium (Ca%, phosphorus (P%, pH and dry matter (DM % of the poultry litter. To evaluate the footpad dermatitis were evaluated eight feet per treatment by visual analysis. At 35 and 42 days of age DM % MM (%, Ca (% P (% and pH of poultry litter no difference (p>0.05 was found between the treatments and the type of ingredient in the birds' diet is not related (p>0.05 with the incidence of footpad dermatitis. The incidence of footpad dermatitis and the quality of the litter weren't influenced by the type of the ingredient used in diet.

  14. High Temperature at Grain-filling Stage Affects Nitrogen Metabolism Enzyme Activities in Grains and Grain Nutritional Quality in Rice

    Institute of Scientific and Technical Information of China (English)

    LIANG Cheng-gang; CHEN Li-ping; WANG Yan; LIU Jia; Xu Guang-li; LI Tian

    2011-01-01

    Rice plants would more frequently suffer from high temperature (HT) stress at the grain-filling stage in future.A japonica rice variety Koshihikari and an indica rice variety IR72 were used to study the effect of high temperature on dynamic changes of glutamine synthetase (GS) activity,glutamate synthase (GOGAT) activity,glutamic oxalo-acetic transminase (GOT) activity,glutamate pyruvate transminase (GPT) activity in grains and grain nutritional quality at the grain-filling stage.Under HT,the activities of GOGAT,GOT,GPT and soluble protein content in grains significantly increased,whereas GS activity significantly decreased at the grain-filling stage.In addition to the increase of protein and amino acids contents,it was suggested that GOGAT,GOT and GPT in grains played important roles in nitrogen metabolism at the grain-filling stage.Since the decrease of GS activity in grains did not influence the accumulations of amino acids and protein,it is implied that GS might not be the key enzyme in regulating glutamine content in grains.

  15. High Temperature at Grain-filling Stage Affects Nitrogen Metabolism Enzyme Activities in Grains and Grain Nutritional Quality in Rice

    Directory of Open Access Journals (Sweden)

    Cheng-gang LIANG

    2011-09-01

    Full Text Available Rice plants would more frequently suffer from high temperature (HT stress at the grain-filling stage in future. A japonica rice variety Koshihikari and an indica rice variety IR72 were used to study the effect of high temperature on dynamic changes of glutamine synthetase (GS activity, glutamate synthase (GOGAT activity, glutamic oxalo-acetic transminase (GOT activity, glutamate pyruvate transminase (GPT activity in grains and grain nutritional quality at the grain-filling stage. Under HT, the activities of GOGAT, GOT, GPT and soluble protein content in grains significantly increased, whereas GS activity significantly decreased at the grain-filling stage. In addition to the increase of protein and amino acids contents, it was suggested that GOGAT, GOT and GPT in grains played important roles in nitrogen metabolism at the grain-filling stage. Since the decrease of GS activity in grains did not influence the accumulations of amino acids and protein, it is implied that GS might not be the key enzyme in regulating glutamine content in grains.

  16. Whole grains and health: from theory to practice--highlights of The Grains for Health Foundation's Whole Grains Summit 2012.

    Science.gov (United States)

    McKeown, Nicola M; Jacques, Paul F; Seal, Chris J; de Vries, Jan; Jonnalagadda, Satya S; Clemens, Roger; Webb, Densie; Murphy, Lee Anne; van Klinken, Jan-Willem; Topping, David; Murray, Robyn; Degeneffe, Dennis; Marquart, Leonard F

    2013-05-01

    The Grains for Health Foundation's Whole Grains Summit, held May 19-22, 2012 in Minneapolis, was the first meeting of its kind to convene >300 scientists, educators, food technologists, grain breeders, food manufacturers, marketers, health professionals, and regulators from around the world. Its goals were to identify potential avenues for collaborative efforts and formulate new approaches to whole-grains research and health communications that support global public health and business. This paper summarizes some of the challenges and opportunities that researchers and nutrition educators face in expanding the knowledge base on whole grains and health and in translating and disseminating that knowledge to consumers. The consensus of the summit was that effective, long-term, public-private partnerships are needed to reach across the globe and galvanize the whole-grains community to collaborate effectively in translating whole-grains science into strategies that increase the availability and affordability of more healthful, grain-based food products. A prerequisite of that is the need to build trust among diverse multidisciplinary professionals involved in the growing, producing, marketing, and regulating of whole-grain products and between the grain and public health communities.

  17. More than Rising Grain Prices

    Institute of Scientific and Technical Information of China (English)

    Hu Junhua

    2010-01-01

    @@ According to the bulletin of the National Bureau of Statistics,the summer harvest nationwide this year was 246.2billion jin,a decline of 0.3% compared to last year.The cold spell in late spring and high temperatures afterwards are considered as the main causes for this round of rising grain prices.However,"natural disasters"are dwarfed by another worrisome picture: young and strong farming hands flooding out of the rural areas and the elderly,the weak,females and children are made the mainstay of the tilling army.

  18. Direct imaging of grain boundaries

    Energy Technology Data Exchange (ETDEWEB)

    Gronsky, R.

    1979-09-01

    There are currently two types of microscopes which, in principle, are capable of imaging atom positions at grain boundaries. One, the field ion microscope (FIM), yields a projection of the specimen surface (approximately stereographic) by field ionization of an imaging gas at protruding atom sites, and provides topographic information in high-index pole regions which may be interpreted atom-by-atom. The other, a transmission electron microscope (TEM), yields a projection (approximately linear) of the entire specimen thickness by electron optical imaging, and provides atomic resolution detail throughout the illuminated area. In this paper, both methods are described and compared, using examples from practical materials systems.

  19. Exocrine pancreatic secretion is stimulated in piglets fed Fish oil compared with those fed Coconut Oil or Lard

    DEFF Research Database (Denmark)

    Hedemann, Mette Skou; Pedersen, Asger Roer; Engberg, Ricarda M.

    2001-01-01

    An experiment was conducted to study the effect of feeding diets containing fat sources with different fatty acid composition (fish oil, coconut oil or lard, 10 g/100 g diet) on exocrine pancreatic secretion in piglets after weaning. A total of 16 barrows were weaned at 4 wk of age; 3 d later...... the coconut oil or lard diets. The output [U/(h. kg(0.75))] of lipase was higher in piglets fed fish oil than in piglets fed lard or coconut oil. The output of colipase was greater in piglets fed fish oil and coconut oil than in those fed lard. The dietary treatments did not affect the output of carboxylester...... hydrolase. The output of trypsin was significantly lower in piglets fed lard than in piglets fed fish oil or coconut oil diets and the output of carboxypeptidase B was greater in those fed the fish oil diet. Protein, chymotrypsin, carboxypeptidase A, elastase and amylase outputs did not differ among...

  20. Roles of grain boundaries in improving fracture toughness of ultrafine-grained metals

    Science.gov (United States)

    Shimokawa, T.; Tanaka, M.; Kinoshita, K.; Higashida, K.

    2011-06-01

    In order to improve the fracture toughness in ultrafine-grained metals, we investigate the interactions among crack tips, dislocations, and grain boundaries in aluminum bicrystal models containing a crack and tilt grain boundaries using molecular dynamics simulations. The results of previous computer simulations showed that grain refinement makes materials brittle if grain boundaries behave as obstacles to dislocation movement. However, it is actually well known that grain refinement increases fracture toughness of materials. Thus, the role of grain boundaries as dislocation sources should be essential to elucidate fracture phenomena in ultrafine-grained metals. A proposed mechanism to express the improved fracture toughness in ultrafine-grained metals is the disclination shielding effect on the crack tip mechanical field. Disclination shielding can be activated when two conditions are present. First, a transition of dislocation sources from crack tips to grain boundaries must occur. Second, the transformation of grain-boundary structure into a neighboring energetically stable boundary must occur as dislocations are emitted from the grain boundary. The disclination shielding effect becomes more pronounced as antishielding dislocations are continuously emitted from the grain boundary without dislocation emissions from crack tips, and then ultrafine-grained metals can sustain large plastic deformation without fracture with the drastic increase of the mobile dislocation density. Consequently, it can be expected that the disclination shielding effect can improve the fracture toughness in ultrafine-grained metals.

  1. Study on the Extraction of Soluble Dietary Fiber From Brewer's Spent Grains%从啤酒糟中提取水溶性膳食纤维的研究

    Institute of Scientific and Technical Information of China (English)

    王异静; 吴会丽

    2007-01-01

    探讨了采用碱法和酶解法提取啤酒糟中水溶性膳食纤维的工艺条件.结果表明,在化学法的最佳条件下,SDF得率为13.37%;在酶法的最佳条件下,SDF得率为4.94%.化学法提取的SDF,颜色较深,为焦糖色;酶法的提取的SDF,颜色较浅,为黄色.

  2. 超声强化提取啤酒糟水溶性多糖的工艺研究%Study on Extraction of Water Soluble Polysaccharide from Brewer's Spent Grain

    Institute of Scientific and Technical Information of China (English)

    朱杰; 李琳; 何元哲; 付全意; 黄玲玲; 别平平; 李冰

    2009-01-01

    为获得啤酒糟水溶性多糖的工艺条件,采用超声强化法提取啤酒糟多糖,确定最佳工艺条件为超声功率180W、超声温度70℃、超声时间15min、液同比25:1(ml/g),在此条件下多糖的得率为5.79%.经过初步纯化,多糖质量分数达到了92.59%.结果表明,超声可有效强化提取啤酒糟水溶性多糖.

  3. Study on enzymatic hydrolysis and ball milling of brewer's spent grain for feed protein peptide%酶解机械预处理啤酒糟制备饲用蛋白肽

    Institute of Scientific and Technical Information of China (English)

    张麟; 蔡国林; 高献礼; 陆健; 董建军; 余俊红; 杨梅

    2014-01-01

    啤酒糟经过粉碎后筛分,筛下物经球磨后粗蛋白含量提高到38.8%,比原样提高了36.1%,水溶性蛋白含量提高到2.53%,比原样提高了3.1倍,经中性蛋白酶液态酶解,水溶性蛋白含量达到12.85%,比原样的7.88%提高了1.6倍,机械预处理显著提高了酶解效率.通过单因素实验和混合水平均匀设计实验,确定了啤酒糟最优酶解条件:加酶量2 400 U/g,酶解温度45℃,酶解pH 8.0,酶解时间8h,此时水溶性蛋白含量能够达到21.31%,提取率达到54.9%.对酶解液进行真空冷冻干燥,制备饲用蛋白肽,粗蛋白含量达到78.8%,氨基酸含量达到74.6%,必需氨基酸含量达到28.18%,多肽含量达到60.8%.

  4. Antibacterial activity of staphylococcus aureus hydrolyzed from brewer's spent grains protein%啤酒糟蛋白水解物对金黄色葡萄球菌抑菌能力研究

    Institute of Scientific and Technical Information of China (English)

    宗绪岩; 李丽; 罗惠波; 刘长江

    2012-01-01

    Four proteases were used in different hydrolysis time and hydrolysis's antibacterial ability was detected. The results showed: hydrolysis has better inhibition ability of Staphylococcus aureus with Flavourzyme in 180 min. Peptides was separated and tested with pol-yamide column chromatography, ion exchange column chromatogra-phy, and gel column chromatography. Molecular weight is about 1 877. 67. The minimal inhibitory concentration for Escherichia coli was 2%.%选择4种蛋白酶制备出不同时间水解的啤酒糟蛋白水解物,分别检测其对金黄色葡萄球菌的抑茵能力.结果表明:采用Flavourzyme水解180 min的水解物具有较好的抑制金黄色葡萄球菌的能力;采用聚酰胺柱层析、离子交换柱层析方法及凝胶柱层析方法对水解物进行分离测定,得到分子量约为1 877.67的啤酒糟多肽样品,其对于金黄色葡萄球菌的最小抑菌浓度为2%.

  5. Optimization of Fermentation Conditions of Ferulic Acid Release from Brewers' Spent Grain by Actinomycosis%放线菌降解麦糟释放阿魏酸的发酵培养条件优化

    Institute of Scientific and Technical Information of China (English)

    程珊影; 李夏兰; 陈志燕; 方柏山

    2010-01-01

    应用均匀设计法设计和二次多项式逐步回归分析,对一株放线菌降解麦糟释放反式阿魏酸的发酵培养条件进行优化.由实验得出最优发酵培养条件:发酵时间为120 h,温度为28 ℃,摇瓶转速为220 r·min~(-1),装液量为30 mL,接种量为1 mL,初始pH值为8.0.然后,优化发酵培养基的碳源和氮源配方:麦糟质量浓度为120 g·L~(-1),麦糟粒径为0.054 mm.在此基础上,反式阿魏酸的最高释放率为25.38%,比发酵培养工艺优化前提高152.0%,而重要的是,放线菌利用麦糟释放阿魏酸的发酵培养基中不需要另外加入酵母粉.

  6. Research on production of protein feedstuff from brewer's spent grain by mixed culture solid-state fermentation%混菌固态发酵啤酒糟生产蛋白饲料的研究

    Institute of Scientific and Technical Information of China (English)

    王颖; 马海乐

    2010-01-01

    对啤酒糟资源的更深层开发利用,提高其饲喂效价,是缓解我国蛋白质资源短缺的有效途径之一.通过单因素试验和正交优化试验,确定混菌种发酵啤酒糟生产高蛋白饲料的最佳发酵条件啤酒糟与豆粕配比为7:3,接种量为20%,硫酸铵添加量为0.5%,尿素添加量为0.5%,发酵时间为1d.在此条件下,真蛋白达到39.13%.

  7. 啤酒糟在国内外食品加工中的利用现状%Current utilization of brewer's spent grain in food processing at home and abroad

    Institute of Scientific and Technical Information of China (English)

    郭萌萌; 张开利; 杜金华; 金玉红; 刘成

    2013-01-01

    啤酒糟是啤酒酿造的最主要副产物,含有丰富的蛋白质和非淀粉多糖.随着啤酒原料中小麦芽使用量的增加(≥40%),啤酒糟中膳食纤维和蛋白质的含量明显增加.目前啤酒糟主要用作饲料或工业原料出售,但在食品加工中的应用研究较少.本文通过概述啤酒糟的营养、加工处理方法及其在国内外食品加工领域中的研究利用情况,为高蛋白膳食纤维啤酒糟食品的进一步开发提供了参考.

  8. 粉碎及酶处理对啤酒糟理化特性的影响%Effect of grinding and enzymatic treatment on the physical and chemical properties of brewer's spent grain

    Institute of Scientific and Technical Information of China (English)

    张麟; 蔡国林; 高献礼; 陆健

    2013-01-01

    对啤酒糟进行机械预处理可以改变啤酒糟的物理结构,使得抗营养因子更容易降解,提高其生物利用率,改善饲用品质.采用球磨和刀片式粉碎预处理酒糟,粒径分布分别在300~1 000 μm和10 ~ 200 μm.经球磨和刀片粉碎后的啤酒糟可溶性总糖含量分别提高了2.2倍和1.7倍,还原糖含量分别提高了40%和18.9%.球磨和刀片粉碎后的啤酒糟经纤维素酶、中性蛋白酶分别酶解后,可溶性总糖含量比啤酒糟原样经酶解后分别提高了2.5倍和1.7倍,还原糖含量分别提高了2.2倍和1.4倍,水溶性蛋白含量分别提高了1.8倍和1.4倍,多肽含量分别提高了2.2倍和1.2倍.经球磨后的啤酒糟酶解效率高于经刀片粉碎的啤酒糟,刀片粉碎并筛分可以初步实现蛋白-纤维的富集分离.

  9. 不同提取方法提取的啤酒糟蛋白质氨基酸组成比较%Comparison of amino acids composition of brewer's spent grain protein isolated by different extract method

    Institute of Scientific and Technical Information of China (English)

    何元哲; 李冰; 田应娟; 唐德松; 李琳

    2010-01-01

    应用全自动氨基酸分析仪分别对啤酒糟原料、啤酒糟碱溶蛋白和啤酒糟醇溶蛋白进行氨基酸组成及含量分析.结果表明,两种提取方法对啤酒糟蛋白和氨基酸的提取具有组分选择性:碱提法主要提出啤酒糟麦谷蛋白,而醇溶法主要提出麦醇溶蛋白,相对于啤酒糟原料和啤酒糟碱溶蛋白,啤酒糟醇溶蛋白中的氨基酸组成特征更明显,具有显著的B类麦醇溶蛋白的特征氨基酸含量:Glu31.18%,Pro 18.92%.Lysl.8%.

  10. 啤酒糟和麸皮袋装混贮品质分析%The Analysis of Mixed Silage quality of Brewer's Spent Grains(BSG) and Bran

    Institute of Scientific and Technical Information of China (English)

    时建青; 徐红蕊; 艾尼瓦尔艾山; 赵国琦

    2005-01-01

    将啤酒糟和麸皮按不同比例袋装混贮,30天开袋,取样进行感官评定,氨态氮占总氮比(NH3-N/tN%)、pH值、干物质(DM)、粗蛋白(CP)、中性洗涤纤维和酸性洗涤纤维(NDF和ADF)及有机酸含量分析.结果表明:水分含量对发酵效果有显著影响,以含水量50%、60%的啤酒糟和麸皮混贮效果最好,其次是40%,70%最差.

  11. 用麦芽根调节水分对啤酒糟混贮品质的影响%Effect of Malty Root Regulating Moisture on Mixed Silage of Brewer's Spent Grains (BSG)

    Institute of Scientific and Technical Information of China (English)

    尹召华; 时建青; 姜婷; 赵国琦

    2006-01-01

    将啤酒糟和麦芽根按不同比例袋装混贮,分别在30、60、90、120 d开袋取样进行感官评定,pH值、氨态氮、干物质、粗蛋白、中性洗涤纤维及有机酸含量分析.结果表明:混贮时间和水分含量均对混贮效果有显著影响;混贮饲料在混贮前60 d各指标变化范围较大,60 d后变化范围较小,并逐渐趋于稳定状态;混贮饲料以含水量50%的袋装混贮效果最好,其次是60%、40%,70%最差.

  12. 啤酒糟发酵产纤维素酶的条件优化研究%Ideal Growth Conditions for Cellulase Enzymatic Produced Using Brewer's Spent Grain Through Fermentation

    Institute of Scientific and Technical Information of China (English)

    宾冬梅; 黄河清涛; 伍渡清; 易诚; 饶力群

    2015-01-01

    为优化发酵条件,选择黑曲霉及康氏木霉,以羧甲基纤维素酶活性为指标,通过正交优化试验,优化单一菌种和混和菌种的最佳发酵条件,比较发酵结果.结果表明:黑曲霉在时间为3天,料水比1:1.5,接种量20%,温度34℃的条件下发酵,测量结果为纤维素酶活为706.427 nkat;康氏木霉在时间为3天,料水比1:1.5,接种量30%,温度34℃的条件下发酵,测量结果为纤维素酶活为459.230 nkat;混菌发酵中,料水比为1:0.5,混菌接种20%,其中黑曲霉比木霉接种量为6:4,在32℃下培养3天时,纤维素酶活为812.380 nkat.表明混菌发酵效果比单菌种发酵效果更好,所产生的纤维素酶活更高.

  13. 啤酒糟-玉米膨化食品的双螺杆挤压工艺与配方%Study on Cooking Extrusion Conditions and Formula of Brewer's Spent Grain-corn Snack with Twin-screw Extruder

    Institute of Scientific and Technical Information of China (English)

    郭萌萌; 耿赟; 郝夕祥; 郑路; 杜金华

    2010-01-01

    以玉米、啤酒糟为原料,进行双螺杆挤压膨化试验,开发新型酒糟膨化食品的同时提高啤酒糟的利用效率.以膨化产品的膨化率、硬度和感官特征做为考核指标,由单因素试验结果确定啤酒糟添加量(A)、物料水分(B)、螺杆转速(C)和挤压温度(D)的最佳水平.在单因素试验基础上采用正交试验设计,优化膨化工艺.试验结果表明:在喂料速度25 kg/h时,啤酒糟添加量10%,物料水分17%,螺杆转速300 r/min,挤压温度130℃的条件下,膨化效果最好.与未添加啤酒糟的玉米膨化产品相比,蛋白质和膳食纤维含量明显提高.

  14. 利用啤酒麦糟进行L-乳酸生产的研究%Study on of Preparation of L-Lactic Acid with Brewer's Spent Grains

    Institute of Scientific and Technical Information of China (English)

    邵荣; 余晓红; 刘珊珊

    2008-01-01

    将湿啤酒糟烘干至含水量为10%左右,再进行机械粉碎,过20~40目筛子筛分后,采用0.80%硫酸,固液比1:11,浸泡原料10h后,于121℃下预水解90min.预水解物pH值用2.0%的氧氧化钠调至4.0左右后,添加0.20%(W/V)左右的纤维素酶,于50℃酶解5h,得到还原糖含量为8.82%的啤酒糟酶解液.将此酶解液pH调节到6.0左右,于55℃采用分批补料的发酵方式进行发酵控制,添加5.0%的碳酸钙,可得到乳酸产量为80.75g/L的发酵液.

  15. Studies on Producing Cellulase from Brewer's Spent Grain through Solid Fermentation by Trichoderma viride%绿色木霉固态发酵啤酒糟生产纤维素酶的研究

    Institute of Scientific and Technical Information of China (English)

    林建国; 胡瑛; 王常高; 蔡俊

    2009-01-01

    以啤酒糟为主要原料,采用绿色木霉(Trichoderma viride)固态发酵生产纤维素酶,对培养基的组成和培养条件进行了优化.实验结果表明,发酵培养基组分为:500 mL三角瓶中装入啤酒糟和麸皮30 g,配料比8∶2,料水比1∶1.5,在30℃发酵66 h,滤纸酶活和羧甲基纤维素酶活分别达到577.0±4.1 u/g和9818.9±4.2 u/g(干物质);而在含氮量相等的条件下,实验所添加的几种无机氮对酶活影响不显著.

  16. 脂肪酶水解麦糟中脂肪的反应动力学研究%Reaction Dynamics of Liqid Hydrolysis from Brewer's Spent Grains with Lipase

    Institute of Scientific and Technical Information of China (English)

    许彬; 李慧星; 肖连冬; 郝同飞

    2016-01-01

    脱脂是制备麦糟膳食纤维的重要工序.实验利用脂肪酶水解麦糟中脂肪,采用单因素实验和响应曲面法考察并优化影响反应速率的因素.在优化的条件下,依据中间复合物学说,建立酶促动力学方程.结果表明:在pH 7.0,温度40℃,加酶量0.32g,CMg2+为0.108 mol/L条件下,反应速率达0.67 mg/mL·h,酶促动力学方程为(dCs/dt)=V=(1.4×Cs/Cs+6.288).结果可为应用脂肪酶水解麦糟中脂肪的生产强化和工程设计提供技术参考.

  17. 啤酒糟蛋白抗氧化肽的酶法制备及其体外抗氧化活力%Enzymatic Preparation and Antioxidant Activity in vitro of Brewer's Spent Grain Antioxidant Peptides

    Institute of Scientific and Technical Information of China (English)

    宗绪岩; 刘长江; 李丽; 刘玲

    2010-01-01

    为了更好地了解和研究啤酒糟蛋白的酶水解特性,选取了4种微生物蛋白酶(Alcalase、Flavourzyme、Neutrase和Protamex)对啤酒糟蛋白进行水解试验,并监测反应过程中水解度的变化和中间产物的还原力改变.结果表明:Alcalase的水解啤酒糟蛋白能力最强.在水解接近3h时基本达到DHmax.水解度的变化同还原力的变化并不完全符合对应关系,在接近DHmax时,还原力的变化随着水解的进行波动很大,还原力的极值点也基本都出现在水解度曲线的拐点附近,说明抗氧化活力同水解度的变化并不完全符合对应关系.

  18. The Effect of Different Physical Methods on the Solubility of Brewers' Spent Grain's Protein%不同物理方法对啤酒糟蛋白溶解性的影响

    Institute of Scientific and Technical Information of China (English)

    刘长江; 宣丽; 宗绪岩; 刘玲; 许金光

    2010-01-01

    为了提高啤酒糟蛋白的溶解性,使它更广泛的应用于食品行业,研究了水浴加热和超声波处理对啤酒糟蛋白溶解性的影响.通过单因素试验,确定了水浴加热处理和超声波处理的最佳改性条件.通过水浴和超声波的交互试验确定了各因素影响的主次顺序,最后确定了提高啤酒糟蛋白溶解性的最佳工艺条件为:水浴温度100℃,蛋白浓度7%,超声时间60s,在此条件下,啤酒糟蛋白的溶解性与对照相比可提高866%,其中水浴温度、蛋白浓度以及水浴温度与蛋白浓度的交互作用是影响啤酒糟蛋白溶解性的高度显著因素.

  19. 啤酒糟醇溶蛋白酶法水解及水解产物的抗氧化研究%Enzymatic Hydrolysis of Alcohol Soluble Protein from Brewer's Spent Grain and the Antioxidant Properties of the Hydrolysates

    Institute of Scientific and Technical Information of China (English)

    唐德松; 何元哲; 李冰; 李琳; 张朝华

    2010-01-01

    用碱性蛋白酶(Alcalase)对啤酒糟醇溶蛋白进行水解,并使用正交试验设计以水解度为指标对酶法水解进行了优化.结果表明,啤酒糟醇溶蛋白的酶解最优条件为底物浓度2%,酶解温度60℃,pH 9.5,酶浓度(E/S)0.096 Au/g,酶解时间3h.以DPPH自由基清除率和羟自由基清除率为指标,用抗坏血酸做对照,对酶解产物的抗氧化活性进行了分析.分别得到了两种自由基清除的最优酶解条件.啤酒糟醇溶蛋白酶解产物对不同自由基的最佳清除作用的水解条件不一致,可能与所产生的多肽对几种自由基的清除机理有关.

  20. The effect of cellulose on the rate of enzymatichydrolysis of protein   of brewer's spent grains%纤维素酶对啤洒糟蛋白酶解率的影响

    Institute of Scientific and Technical Information of China (English)

    邱雁临

    2001-01-01

    本研究对单酶和双酶水解啤酒糟蛋白及添加纤维素酶预处理原料等进行了对比试验,优选出双酶水解的最佳组合,试验表明,用纤维素酶预处理原料可提高啤酒糟蛋白酶解率10%以上.

  1. [Study of the nutritive value of grain proteins using Tribolium confusum larvae].

    Science.gov (United States)

    Balaian, V M; Levitskiĭ, A P

    1985-01-01

    A study was made of the nutrition of Tribolium confusum larvae with the purpose of using them as test-organism for determination of the nutritive value of grain proteins. It was demonstrated that there is a good agreement between the content of lysine in the grain diets containing barley and corn and average daily weight gains among the insects: r = +0,85 and r = +0,75, respectively. It was noted that during nutrition with grain diets containing different levels of proteins, the growth of the insects depended on the protein quality. Experiments on the insects fed diets prepared from different anatomical parts of the grain demonstrated a high nutritive value of germ proteins. Comparative experiments performed with the diets from barley demonstrated a good agreement between average daily weight gains of rats and insect larvae (r = +0.9). The data obtained indicate that the test-organism under consideration can be used for evaluating the nutritive value of grain proteins.

  2. Degradability Characteristics of Treated and Untreated Barley Grain Using In situ Technique

    Directory of Open Access Journals (Sweden)

    Akbar Taghizadeh

    2008-01-01

    Full Text Available This study was carried out to determine of degradability characteristics of untreated barely grain (UBG and treated barley grain with autoclaving at 120°C, 5´ (TBG1 and 20' (TBG2, treated barley grain at 100°C, 5´ (TBG3 and 20' (TBG4, using in situ technique in Ghizel sheep’s. The sheep fed diet content 40% alfalfa: 60% concentrate containing 2.9 Mcal kg-1 DM and 14% CP. The incubation times were 0, 4, 8, 16, 24, 36 and 48 h and rumen degradation of cp and DM was measured. The equation of p = a+b (1-e-ct was used for fitting of dry matter and crude protein disappearance data. The dry matter disappearance of TBG1 and TBG2 at 24 and 48 h were lower than the other treatments (p<0.05. The crude protein disappearance of 24 and 48 h of UBG was more than the other treatment (p<0.05. Treating of barley grain of 120°C (5' and 20' can be decreased ruminal crude protein degradability of barley grain resulting high escaped crude protein into lower digestive tract.

  3. Digestible indispensable amino acid score and digestible amino acids in eight cereal grains.

    Science.gov (United States)

    Cervantes-Pahm, Sarah K; Liu, Yanhong; Stein, Hans H

    2014-05-01

    To determine values for the digestible indispensable amino acid score (DIAAS), it is recommended that ileal amino acid (AA) digestibility values obtained in growing pigs are used to characterise protein quality in different foods. Therefore, an experiment was conducted to determine the standardised ileal digestibility (SID) of AA in eight cereal grains (yellow dent maize, Nutridense maize, dehulled barley, dehulled oats, polished white rice, rye, sorghum and wheat) fed to pigs, where SID values in pigs can be used to calculate approximate DIAAS values in humans. In the present experiment, twenty-four barrows with a T-cannula inserted in the distal ileum were allotted to eight diets and fed for three periods to give a total of nine replicate pigs per diet. Each period lasted 14 d, and ileal digesta samples were collected on days 13 and 14. Among the SID values obtained for all cereal grains, values for total indispensable AA were greatest (P< 0·05) in rice and lowest (P< 0·05) in rye and sorghum. The concentrations of SID indispensable AA in rice were less (P< 0·05) than in dehulled oats, but greater (P< 0·05) than in the other cereal grains, and the concentrations of SID indispensable AA in Nutridense maize were greater (P< 0·05) than in yellow dent maize and sorghum, but less (P< 0·05) than in the other cereal grains, except rye. In conclusion, results indicate that to meet dietary requirements for AA in humans, diets based on yellow dent maize or sorghum require more AA supplementation than diets based on other cereal grains.

  4. Effects of feeding pelleted diets without or with distillers dried grains with solubles on fresh belly characteristics, fat quality, and commercial bacon slicing yields of finishing pigs.

    Science.gov (United States)

    Overholt, M F; Lowell, J E; Wilson, K B; Matulis, R J; Stein, H H; Dilger, A C; Boler, D D

    2016-05-01

    One hundred ninety-two pigs were blocked by age and stratified by initial BW (25.7 ± 2.3 kg) into pens (2 barrows and 2 gilts/pen), and within blocks, pens were assigned randomly to 1 of 4 treatments in a 2 × 2 factorial arrangement, with main effects of diet form (meal vs. pelleted) and distillers dried grains with solubles (DDGS) inclusion (0% vs. 30%). Pigs were slaughtered after a 91-d feeding trial, and carcasses were fabricated after a 24-h chilling period. Belly dimensions and flop distance were measured, and an adipose tissue sample from each belly was collected for fatty acid analysis. Bacon was manufactured at a commercial processing facility before being returned to the University of Illinois Meat Science Laboratory for further evaluation. Although bellies from pigs fed pelleted diets were 5.3% heavier ( bacon weight and cooked yield were greater ( ≤ 0.01) for bellies from pellet-fed than meal-fed pigs. Despite pellet-fed pigs having a 3.1-unit greater iodine value (IV) than meal-fed pigs, there was no effect ( ≥ 0.16) of diet form on commercial bacon slicing yields. Bacon slabs from pellet-fed pigs produced more ( bacon slices, but 3.1% fewer ( bacon slices than feeding 30% DDGS. Distillers dried grains with solubles inclusion had no effect on slice yields ( ≥ 0.14) or slices per kilogram ( = 0.08). Overall, bellies from pellet-fed pigs were heavier and had greater IV but did not differ in commercial slicing yields from meal-fed pigs. Feeding pigs 30% DDGS produced thinner, softer bellies with greater IV, but slicing yields were not different from bellies of pigs fed 0% DDGS. Thus, swine producers can feed pelleted diets, without or with 30% DDGS, without negatively affecting commercial bacon slicing yield.

  5. 77 FR 76452 - Grain Inspection Advisory Committee Reestablishment

    Science.gov (United States)

    2012-12-28

    ... Doc No: 2012-31281] DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration Grain Inspection Advisory Committee Reestablishment AGENCY: Grain Inspection, Packers and Stockyards... of Agriculture has reestablished the Grain Inspection, Packers and Stockyards Administration (GIPSA...

  6. Whole Grains: Hearty Options for a Healthy Diet

    Science.gov (United States)

    ... Lifestyle Nutrition and healthy eating Find out why whole grains are better than refined grains and how to ... your diet. By Mayo Clinic Staff Grains, especially whole grains, are an essential part of a healthy diet. ...

  7. Converter fed sub sea motor drives

    Energy Technology Data Exchange (ETDEWEB)

    Raad, R.O.

    1995-09-01

    Minor offshore gas and oil resources located 20-50 km from existing installations may often be commercially exploited only by use of complete sub sea solutions. This thesis deals with analyses of a sub sea adjustable speed electric motor which is fed by a frequency converter via a long cable (up to 50 km) between the converter and the motor. The author develops a general model for analysing such motor drive systems with the objective of verifying the feasibility of specific applications and of specifying the requirements on the system components. The simulation model is used to identify the critical frequency ranges in which the converter must not generate significant harmonics, to verify the start-up strategy chosen, and to verify the stability with potential disturbances applied to the system. Simulation models are developed for both transient and steady state analyses. They are accurate up to 5 kHz and can incorporate the frequency dependency of the motor and cable parameters. Ideal thyristors and diodes are used. The models are implemented in existing simulation tools. Most of the results relate to a base case with a 670 kW squirrel cage motor fed from a 30 km long cable, but cases with 3 MW rating or with 50 km cable have also been analyzed and found to be feasible. Each specific application must be separately studied. Results of simulation calculations are presented and conclusions given. 53 refs., 124 figs., 23 tabs.

  8. Coupling Between Waveguide-Fed Slot Arrays

    Science.gov (United States)

    Rengarajan, Sembiam

    2011-01-01

    Coupling between two waveguide-fed planar slot arrays has been investigated using full-wave analysis. The analysis employs the method-of-moments solution to the pertinent coupled integral equations for the aperture electric field of all slots. In order to compute coupling between two arrays, the input port of the first array is excited with a TE(sub 10) mode wave while the second one is match-terminated. After solving the moment method matrix equations, the aperture fields of all slots are obtained and thereby the TE(sub 10) mode wave received at the input port of the second array is determined. Coupling between two arrays is the ratio of the wave amplitude arriving in the second array port to the incident wave amplitude at the first array port. The coupling mechanism has been studied as a function of spacing between arrays in different directions, e.g. the electric field plane, the magnetic field plane, and the diagonal plane. Computed coupling values are presented for different array geometries. This work is novel since it provides a good understanding of coupling between waveguide-fed slot arrays as a function of spacing and orientation for different aperture distributions and array architectures. This serves as a useful tool for antenna design engineers and system engineers.

  9. Grain alignment in starless cores

    Energy Technology Data Exchange (ETDEWEB)

    Jones, T. J.; Bagley, M. [Minnesota Institute for Astrophysics, University of Minnesota, Minneapolis, MN 55455 (United States); Krejny, M. [Cree Inc., 4600 Silicon Dr., Durham, NC (United States); Andersson, B.-G. [SOFIA Science Center, USRA, Moffett Field, CA (United States); Bastien, P., E-mail: tjj@astro.umn.edu [Centre de recherche en astrophysique du Québec and Départment de Physique, Université de Montréal, Montréal (Canada)

    2015-01-01

    We present near-IR polarimetry data of background stars shining through a selection of starless cores taken in the K band, probing visual extinctions up to A{sub V}∼48. We find that P{sub K}/τ{sub K} continues to decline with increasing A{sub V} with a power law slope of roughly −0.5. Examination of published submillimeter (submm) polarimetry of starless cores suggests that by A{sub V}≳20 the slope for P versus τ becomes ∼−1, indicating no grain alignment at greater optical depths. Combining these two data sets, we find good evidence that, in the absence of a central illuminating source, the dust grains in dense molecular cloud cores with no internal radiation source cease to become aligned with the local magnetic field at optical depths greater than A{sub V}∼20. A simple model relating the alignment efficiency to the optical depth into the cloud reproduces the observations well.

  10. Supplementation of hydroxypropyl methylcellulose into yeast leavened all-whole grain barley bread potentiates cholesterol-lowering effect.

    Science.gov (United States)

    Kim, Hyunsook; Turowski, Maciej; Anderson, W H Kerr; Young, Scott A; Kim, Yookyung; Yokoyama, Wallace

    2011-07-27

    We investigated in Syrian Golden hamsters the biological impact and its underlying mechanism of single whole grain breads supplemented with 2-3% hydroxypropyl methylcellulose (HPMC), a semisynthetic viscous soluble dietary fiber (SDF) as a substitute for gluten. Hamsters were fed high-fat diets supplemented with 48-65% (w/w) differently ground, freeze-dried single grain breads including whole grain wheat, barley, barley supplemented with HPMC, debranned oat, and oat supplemented with HPMC which were compared to a diet containing microcrystalline cellulose (control). All single grain breads significantly lowered plasma LDL-cholesterol concentrations compared to the control. Enrichment with HPMC further lowered plasma and hepatic cholesterol concentrations. Despite the reduced molecular weight of naturally occurring soluble (1--->3),(1--->4)-β-d-glucan (β-glucan) caused by the bread-making process, whole grain barley breads downregulated hepatic expression of CYP7A1 and HMG-CoAR genes that are responsible for bile acid and cholesterol synthesis, suggesting a possible role of bioactive compounds such as short-chain fatty acids and phenolic compounds from barley bread. Barley bread enriched with HPMC downregulated expression of ABCG5 gene. Taken together, it appears that distinctive modulation of synthesis and excretion of hepatic cholesterol and bile acid contributes to the cholesterol-lowering properties of whole grain barley breads and breads enriched with HPMC. These data suggests that alternative whole grain breads supplemented with HPMC may provide consumers with a staple food that can assist in cholesterol management.

  11. Effects of different cereal grains in diets for laying hens on production parameters and liver fat content.

    Science.gov (United States)

    Kim, S M; Patel, M B; Reddy, S J; McGuinnis, J

    1976-03-01

    Two experiments were conducted with White Leghorn laying hens to study the effects of different cereal grains on production criteria and liver fat content. The results of Experiment 1, in which pullets 21 weeks of age were used for a period of 22 weeks indicated that Gaines wheat or triticale (Trailblazer) were equal to corn in supporting egg production, egg weight and body weight, with comparable feed consumption. Henry wheat(a hard red winter class) was slightly, yet significantly (P less than 0.05) inferior to corn for the above criteria. No significant differences were observed among four treatments in wet liver weight and liver fat content. Hens fed the corn diet had significantly (P less than 0.05) lower carcass fat followed by the hens fed triticale in comparison with those fed Gaines or Henyry wheat. Mortality was very low and not related to dietary treatments. Neither dietary fat nor energy content was related to fat content of liver and carcass of the hens. Body weight and liver fat content were not closely related to each other. Wet liver weight was the only significantly (P less than 0.05) related factor to liver fat content. In the second experiment, in which hens 33 weeks of age were used for an experimental period of 20 weeks, opaque-2 corn was slightly superior to normal corn and triticale was comparable to normal corn in supporting egg production and egg weight. Supplementation of the diets containing the two corns and triticale with lysine failed to improve egg production and egg weight. Hens fed the diets containing either normal corn or opaque-2 corn as the only grain in the diet had significantly (P less than 0.05) higher liver fat content in comparison with hens fed the diet containing triticale as the only grain. Mortality, however, was much higher among hens fed triticale-containing diets in comparison with groups fed corn-containing diets in spite of the fact that they had significantly lower liver fat content. Regardless of dietary

  12. Exocrine pancreatic secretion is stimulated in piglets fed fish oil compared with those fed coconut oil or lard.

    Science.gov (United States)

    Hedemann, M S; Pedersen, A R; Engberg, R M

    2001-12-01

    An experiment was conducted to study the effect of feeding diets containing fat sources with different fatty acid composition (fish oil, coconut oil or lard, 10 g/100 g diet) on exocrine pancreatic secretion in piglets after weaning. A total of 16 barrows were weaned at 4 wk of age; 3 d later, they were surgically fitted with a catheter in the pancreatic duct for continuous collection of pancreatic juice. Collections of pancreatic juice were made every other day starting 4 d postsurgically. Piglets fed the fish oil diet secreted a significantly greater volume of pancreatic juice than piglets fed the coconut oil or lard diets. The output [U/(h. kg(0.75))] of lipase was higher in piglets fed fish oil than in piglets fed lard or coconut oil. The output of colipase was greater in piglets fed fish oil and coconut oil than in those fed lard. The dietary treatments did not affect the output of carboxylester hydrolase. The output of trypsin was significantly lower in piglets fed lard than in piglets fed fish oil or coconut oil diets and the output of carboxypeptidase B was greater in those fed the fish oil diet. Protein, chymotrypsin, carboxypeptidase A, elastase and amylase outputs did not differ among the dietary treatment groups. The apparent digestibilities of nutrients and energy were measured in feces and did not differ among groups. Thus, the greater output of lipase in fish oil-fed piglets did not result in a greater digestibility of fat in this diet.

  13. Influence of conditioning temperature on the performance, nutrient utilisation and digestive tract development of broilers fed on maize- and wheat-based diets.

    Science.gov (United States)

    Abdollahi, M R; Ravindran, V; Wester, T J; Ravindran, G; Thomas, D V

    2010-10-01

    1. The influence of conditioning temperature on the performance, nutrient utilisation and digestive tract development of broilers fed on maize- and wheat-based diets was examined up to 21 d of age. The experimental design was a 2 × 3 factorial arrangement of treatments evaluating two grain types (maize and wheat) and three conditioning temperatures (60°C, 75°C and 90°C). Broiler starter diets, each based on one grain (maize or wheat), were formulated and pelleted at the three temperatures. 2. Increasing conditioning temperature decreased the body-weight gain and feed intake in wheat-based diets, but birds fed on maize-based diets conditioned at 60°C and 90°C had higher body-weight gain and feed intake than those fed on the diet conditioned at 75°C. Increasing conditioning temperature increased feed per body-weight gain in both grain-type diets but improved pellet durability index (PDI) only in wheat-based diets; PDI was unaffected in maize-based diets. 3. In wheat-based diets, increasing conditioning temperature decreased the ileal digestibility of nitrogen and starch. Ileal nitrogen digestibility of maize-based diets conditioned at 60°C and 90°C was higher than at 75°C. Starch digestibility was unaffected by conditioning temperature in maize-based diets. No effect of conditioning temperature was found for apparent metabolisable energy (AME). Increasing conditioning temperature decreased digestible protein and AME intakes in wheat-based diets but, in maize-based diets, birds fed on the diet conditioned at 75°C had lower digestible protein and AME intakes compared to those fed on diets conditioned at 60°C and 90°C. 4. Small intestine was longer in birds fed on diets conditioned at 75°C and 90°C compared with those fed on diets conditioned at 60°C. 5. Overall, the data suggest that while the effects of conditioning temperature on body-weight gain and feed intake of broilers to 21 d of age differed depending on the grain type, feed per body-weight gain

  14. Grain refinement of AZ31 magnesium alloy by electromagnetic stirring under effect of grain-refiner

    Indian Academy of Sciences (India)

    S Y Gao; Q C Le; Z Q Zhang; J Z Cui

    2012-08-01

    The effects of electromagnetic stirring and Al4C3 grain refiner on the grain refinement of semicontinuously cast AZ31 magnesium alloy were discussed in this investigation. The results indicate that electromagnetic stirring has an effective refining effect on the grain size of AZ31 magnesium alloy under the effect of Al4C3 grain refiner. Electromagnetic stirring can `activate’ the Al4C3 particles, resulting in more heterogeneous nucleation sites for the primary -Mg grains. But, longer holding time can `deactivate’ the Al4C3 particles and poison the grain refining effect.

  15. Prediction of HAZ grain size in welding of ultra fine grained steel with different parameters

    Institute of Scientific and Technical Information of China (English)

    Zhao Hongyun; Zhang Hongtao; Li Dongqing; Wang Guodong

    2010-01-01

    The temperature field and thermal cycling curve in the heat-affected zone during welding 400 MPa ultra fine grained steel by plasma arc were simulated using finite element method.The principle of grain growth kinetics was used to predict the grain size in the heat-affected zone under different welding parameters.The simulation results show that the growing tendency of HAZ grain could be controlled by adjusting the welding parameters,but the growth of HAZ grain could not be eliminated at all.The HAZ grain size became small with increasing of the cooling rate and added with increasing of welding current,arc voltage and welding speed.

  16. Determination of grain boundary geometry using TEM

    Energy Technology Data Exchange (ETDEWEB)

    Jang, H.; Farkas, D. (Department of Materials Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0237 (United States)); De Hosson, J.T.M. (Department of Applied Physics, University of Groningen, Nijenborgh 18, 9747 AG, Groningen (Netherlands))

    1992-07-01

    An experimental method to obtain the grain boundary geometry using the transmission electron microscope is presented. The method allows {Sigma} determination including grain boundary plane orientation. In order to determine the specialness of the grain boundary, three different criteria for maximum allowable deviations from exact CSL misorientations were examined. We tested these three criteria from a statistical distribution of grain boundary types in terms of {Sigma}. We compared grain boundary distributions from other studies in Ni{sub 3}Al and found discrepancies among them. It seems that the discrepancy came from the different criteria for special boundaries in {Sigma} determination and different experimental procedures they used. The statistical distribution of grain boundary plane orientations showed that low {Sigma} boundaries ({Sigma}{lt}11) were oriented to the plane of high density of coincident sites.

  17. Grain Boundary Engineering of Electrodeposited Thin Films

    DEFF Research Database (Denmark)

    Alimadadi, Hossein

    of the favorable boundaries that break the network of general grain boundaries. Successful dedicated synthesis of a textured nickel film fulfilling the requirements of grain boundary engineered materials, suggests improved boundary specific properties. However, the textured nickel film shows fairly low......Grain boundary engineering aims for a deliberate manipulation of the grain boundary characteristics to improve the properties of polycrystalline materials. Despite the emergence of some successful industrial applications, the mechanism(s) by which the boundary specific properties can be improved...... is not yet well-understood. This, at least partly, owes to the lack of robust characterization methods for analyzing the nature of grain boundaries including the grain boundary plane characteristics, until recently. In the past decade, significant improvements in the 2-dimensional and 3-dimensional analysis...

  18. Quasiclassical Coarse Graining and Thermodynamic Entropy

    CERN Document Server

    Gell-Mann, Murray; Gell-Mann, Murray; Hartle, James

    2006-01-01

    Our everyday descriptions of the universe are highly coarse-grained, following only a tiny fraction of the variables necessary for a perfectly fine-grained description. Coarse graining in classical physics is made natural by our limited powers of observation and computation. But in the modern quantum mechanics of closed systems, some measure of coarse graining is inescapable because there are no non-trivial, probabilistic, fine-grained descriptions. This essay explores the consequences of that fact: Quantum theory allows for various coarse-grained descriptions some of which are mutually incompatible. For most purposes, however, we are interested in the small subset of ``quasiclassical descriptions'' defined by ranges of values of averages over small volumes of densities of conserved quantities such as energy and momentum and approximately conserved quantities such as baryon number. The near-conservation of these quasiclassical quantities results in approximate decoherence, predictability, and local equilibriu...

  19. 78 FR 33224 - Safety Zone; Grain-Shipment and Grain-Shipment Assist Vessels, Columbia and Willamette Rivers

    Science.gov (United States)

    2013-06-04

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Grain-Shipment and Grain-Shipment Assist... and outbound grain-shipment and grain-shipment assist vessels involved in commerce with the Columbia Grain facility on the Willamette River in Portland, OR, the United Grain Corporation facility on the...

  20. Nutrient digestibility of veal calves fed large amounts of different solid feeds during the first 80 days of fattening

    Directory of Open Access Journals (Sweden)

    Marta Brscic

    2014-10-01

    Full Text Available The study aimed at evaluating nutrients apparent digestibility in veal calves fed 3 feeding plans based on milk-replacer plus large amounts of solid feeds differing in their composition during the first 80 days of fattening. Twelve Polish Friesian male calves (70.6±1.9 kg were randomly assigned to one of the following feeding treatments: i milk-replacer plus corn grain (CG; ii milk-replacer plus 80:20 mixture (as fed basis of corn grain and wheat straw (CGS; and iii milk-replacer plus 72:20:8 mixture of corn grain, wheat straw and extruded soybean (CGSES. Calves received the same milk-replacer but the daily amount was restricted (96% for CGSES calves to balance dietary protein. Total dry matter intake from milk-replacer and solid feeds was similar among treatments, but CGSES calves showed better growth performance than CG ones. Calves were introduced into a metabolism stall (1/pen during week 9 of fattening for a 3- day adaptation period and a 4-day digestibility trial. Calves fed CG showed the greatest DM, NFC, and ash digestibility while CGSES calves showed the lowest CP digestibility. Haemoglobin concentrations measured at day 5, 31 and 80 were similar among feeding treatments and significantly decreased over time. In CGSES treatment, the combination of milkreplacer with solid feed closer to a complete diet for ruminants led to better calves’ growth performance. However, the reduced protein digestibility with CGSES indicates that protein quality becomes a key factor when formulating diets for veal calves using alternatives to dairy sources.

  1. Grain boundaries in silicon solar cells

    Science.gov (United States)

    Kazmerski, L. L.; Russell, P. E.; Ireland, P. J.; Herrington, C. R.; Dick, J. R.; Matson, R. J.; Jones, K. M.

    The correlations between the electrical and compositional properties of grain boundaries in polycrystalline Si are examined in detail. High-resolution surface analysis techniques (AES, SIMS, XPS, EELS) and microelectrical (SAM, EBIC, minority-carrier lifetime) characterization methods are used. The direct evidence for impurity segregation to the intergrain regions is presented. Effect of illumination on the grain boundary electrical characteristics are correlated with impurity compositions. Finally, the interrelationships among heat-treatment, oxygen segregation and grain boundary electrical activity are discussed.

  2. Grain legumes in organic cropping systems

    OpenAIRE

    Hauggaard-Nielsen, Dr. Henrik

    2002-01-01

    Grain legumes are valuable protein and energy sources in animal feeds and in human diets low in meat. Furthermore, grain legumes strongly benefit the cropping system, via biological fixation of atmospheric N2 - a fundamental process for maintaining soil fertility in organic farming systems. Other positive effects in the crop rotations are recycled N-rich crop residues and the break-crop effect in cereals-rich rotations. However, yield variability in grain legumes is well known and related to...

  3. Interstellar Extinction by Spheroidal Dust Grains

    OpenAIRE

    Gupta, Ranjan; Mukai, Tadashi; Vaidya, D. B.; Sen, Asoke K.; Okada, Yasuhiko

    2005-01-01

    Observations of interstellar extinction and polarization indicate that the interstellar medium consists of aligned non-spherical dust grains which show variation in the interstellar extinction curve for wavelengths ranging from NIR to UV. To model the extinction and polarization, one cannot use the Mie theory which assumes the grains as solid spheres. We have used a T-matrix based method for computing the extinction efficiencies of spheroidal silicate and graphite grains of different shapes (...

  4. 啤酒酵母RNA对5-氟尿嘧啶处理小鼠免疫功能的影响%Effects of Brewer's Yeast RNA on Immune Function in Mice Treated with 5-Fluorouracil

    Institute of Scientific and Technical Information of China (English)

    曾伶; 薛存宽; 何学斌; 陈艳雯; 许明旺; 李颖

    2009-01-01

    Objective To investigate the effects of brewer's yeast RNA on immune insufficiency in mice treated with 5-flu-orouracil. Methods The spleen and thymus index.phagocytosis rate of peritoneal macrophages,levels of serum hemolysin,contents of serum TNF-α and IL-2,and lymphocyte transformation rate were measured after mice were treated with 5-fluorouracil [25 mg/(kg ? d)] and/or brewer's yeast RNA [100 mg/(kg ? d) ,300 mg/(kg ? d) ,respectively] for 14 days. Results The phagocytosis rate of peritoneal macrophages, levels of serum TNF-α and IL-2. and spleen and thymus index in brewer' s yeast RNA-treated groups were significantly higher than those in 5-fluorouracil-treated group (P<0. 05,or P<0. 01). As compared with 5-fluorouracil-treated group,the levels of serum hemolysin and lymphocyte transformation rate were notably increased in brewer's yeast RNA-treated groups (P<0. 05,or P<0. 01). Conclusion Brewer's yeast RNA may increase the immune function of the mice with immune insufficiency.%目的 探讨啤酒酵母RNA对5-氟尿嘧啶(5-Fu)处理小鼠免疫功能的影响.方法 将小鼠随机分为空白组,5-氟尿嘧啶组,啤酒酵母RNA小剂量组、大剂量组[分别以啤酒酵母RNA 100 mg/(kg·d)、300 mg/(kg·d)灌胃给药];除空白组外,其余各组均以5-Fu 25 mg/(kg·d)灌胃.检测腹腔巨噬细胞吞噬率,血清溶血素形成水平、肿瘤坏死因子-α(TNF-α)及白细胞介素-2(IL-2)的含量和淋巴细胞转化率,计算脾指数及胸腺指数.结果 啤酒酵母RNA大、小剂量组均可明显提高5-氟尿嘧啶处理小鼠腹腔巨噬细胞吞噬率、血清TNF-α、IL-2水平,逆转脾指数、胸腺指数的萎缩,与5-氟尿嘧啶组相比差异具有统计学意义(P<0.05或P<0.01);啤酒酵母RNA大、小剂量组对5-氟尿嘧啶所致的血清溶血素形成水平低下和淋巴细胞转化率低下有明显的拮抗作用(P<0.05或P<0.01).结论 啤酒酵母RNA可提高免疫功能低下机体的免疫功能.

  5. L-strip proximity fed ga

    Directory of Open Access Journals (Sweden)

    Ashish Singh

    2014-03-01

    Full Text Available In this article, the analysis of dualband L-strip fed compact semi-circular disk microstrip patch antenna has been presented using circuit theory concept. The antenna parameters such as return loss, VSWR and radiation pattern are calculated. The effect of geometric dimensions of the proposed antenna such as length of vertical and horizontal portion of L-strip is investigated. It is found that antenna resonate at two distinct modes i.e. 1.3 GHz and 6.13 GHz for lower and upper resonance frequencies respectively. The bandwidth of the proposed antenna at lower resonance frequency is 6.61% (simulated and 10.64% (theoretical whereas at upper resonance frequency, it is 6.02% (simulated and 9.06 % (theoretical. The theoretical results are compared with IE3D simulation results as well as experimental results and they are in close agreement.

  6. Contour fractal analysis of grains

    Science.gov (United States)

    Guida, Giulia; Casini, Francesca; Viggiani, Giulia MB

    2017-06-01

    Fractal analysis has been shown to be useful in image processing to characterise the shape and the grey-scale complexity in different applications spanning from electronic to medical engineering (e.g. [1]). Fractal analysis consists of several methods to assign a dimension and other fractal characteristics to a dataset describing geometric objects. Limited studies have been conducted on the application of fractal analysis to the classification of the shape characteristics of soil grains. The main objective of the work described in this paper is to obtain, from the results of systematic fractal analysis of artificial simple shapes, the characterization of the particle morphology at different scales. The long term objective of the research is to link the microscopic features of granular media with the mechanical behaviour observed in the laboratory and in situ.

  7. Quantitative comparisons of select cultured and uncultured microbial populations in the rumen of cattle fed different diets

    Directory of Open Access Journals (Sweden)

    Kim Minseok

    2012-09-01

    Full Text Available Abstract Background The number and diversity of uncultured ruminal bacterial and archaeal species revealed by 16S rRNA gene (rrs sequences greatly exceeds that of cultured bacteria and archaea. However, the significance of uncultured microbes remains undetermined. The objective of this study was to assess the numeric importance of select uncultured bacteria and cultured bacteria and the impact of diets and microenvironments within cow rumen in a comparative manner. Results Liquid and adherent fractions were obtained from the rumen of Jersey cattle fed hay alone and Holstein cattle fed hay plus grain. The populations of cultured and uncultured bacteria present in each fraction were quantified using specific real-time PCR assays. The population of total bacteria was similar between fractions or diets, while total archaea was numerically higher in the hay-fed Jersey cattle than in the hay-grain-fed Holstein cattle. The population of the genus Prevotella was about one log smaller than that of total bacteria. The populations of Fibrobacter succinogenes, Ruminococcus flavefaciens, the genus Butyrivibrio, and R. albus was at least one log smaller than that of genus Prevotella. Four of the six uncultured bacteria quantified were as abundant as F. succinogenes, R. flavefaciens and the genus Butyrivibrio. In addition, the populations of several uncultured bacteria were significantly higher in the adherent fractions than in the liquid fractions. These uncultured bacteria may be associated with fiber degradation. Conclusions Some uncultured bacteria are as abundant as those of major cultured bacteria in the rumen. Uncultured bacteria may have important contribution to ruminal fermentation. Population dynamic studies of uncultured bacteria in a comparative manner can help reveal their ecological features and importance to rumen functions.

  8. Interactions between grain boundary faceting, migration and grain rotation: Color group and molecular dynamics simulation approaches

    Science.gov (United States)

    Huang, Yue

    Color group theory and molecular dynamics (MD) simulations were used to study the faceting and rotation of grains in nanocrystalline materials and their interactions. Color group arguments were used to determine symmetry-dictated extrema with respect to misorientation of the grains and with respect to grain boundary normal orientations. MD simulations were used to study the evolution of the system and to elucidate the interactions between grain rotation and faceting in nano-scale systems. The systems of study were fcc bicrystalline systems with two grains sharing their [110] directions. Two geometric parameters were studied: the misorientation between two grains with a common rotation axis in the [110] direction of both grains, and the grain boundary normal orientation of fcc (110) tilt grain boundaries. The symmetry-dictated extremum (SDE) with respect to misorientation around both grains' [110] direction is 90 degrees. The SDE with respect to GB normal orientations for (110) tilt GBs are located on top of the color and classical mirror planes of their dichromatic patterns. By using periodic boundary conditions and a cylindrical embedded grain structure in our simulations, grains are only free to vary the misorientation between grains around the common [110] direction, and the normal of the grain boundaries are always perpendicular to both grains [110] direction. All SDE studied in our simulation are observed to be local energy minimum states. We observed the systems reducing their excess energy through three main modes: forming facets at the boundaries, rotating between the two grains, and reduction of grain boundary area through grain shrinkage. Facets are formed in low-energy grain boundaries and oscillating rotation occurred when the initial misorientation was not a SDE. A new algorithm was developed to quantitatively measure the grain rotation. The ovsered rotations are not rigid-body rotations and have strong interaction with faceting. Systems with lower

  9. Genome-Wide Association Mapping of Yield and Grain Quality Traits in Winter Wheat Genotypes.

    Directory of Open Access Journals (Sweden)

    W Tadesse

    Full Text Available The main goal of this study was to investigate the genetic basis of yield and grain quality traits in winter wheat genotypes using association mapping approach, and identify linked molecular markers for marker assisted selection. A total of 120 elite facultative/winter wheat genotypes were evaluated for yield, quality and other agronomic traits under rain-fed and irrigated conditions for two years (2011-2012 at the Tel Hadya station of ICARDA, Syria. The same genotypes were genotyped using 3,051 Diversity Array Technologies (DArT markers, of which 1,586 were of known chromosome positions. The grain yield performance of the genotypes was highly significant both in rain-fed and irrigated sites. Average yield of the genotypes ranged from 2295 to 4038 kg/ha and 4268 to 7102 kg/ha under rain-fed and irrigated conditions, respectively. Protein content and alveograph strength (W ranged from 13.6-16.1% and 217.6-375 Jx10-4, respectively. DArT markers wPt731910 (3B, wPt4680 (4A, wPt3509 (5A, wPt8183 (6B, and wPt0298 (2D were significantly associated with yield under rain-fed conditions. Under irrigated condition, tPt4125 on chromosome 2B was significantly associated with yield explaining about 13% of the variation. Markers wPt2607 and wPt1482 on 5B were highly associated with protein content and alveograph strength explaining 16 and 14% of the variations, respectively. The elite genotypes have been distributed to many countries using ICARDA's International system for potential direct release and/or use as parents after local adaptation trials by the NARSs of respective countries. The QTLs identified in this study are recommended to be used for marker assisted selection after through validation using bi-parental populations.

  10. On the reduced lifetime of nitrous oxide due to climate change induced acceleration of the Brewer-Dobson circulation as simulated by the MPI Earth System Model

    Science.gov (United States)

    Kracher, D.; Manzini, E.; Reick, C. H.; Schultz, M. G.; Stein, O.

    2014-12-01

    Greenhouse gas induced climate change will modify the physical conditions of the atmosphere. One of the projected changes is an acceleration of the Brewer-Dobson circulation in the stratosphere, as it has been shown in many model studies. This change in the stratospheric circulation consequently bears an effect on the transport and distribution of atmospheric components such as N2O. Since N2O is involved in ozone destruction, a modified distribution of N2O can be of importance for ozone chemistry. N2O is inert in the troposphere and decays only in the stratosphere. Thus, changes in the exchange between troposphere and stratosphere can also affect the stratospheric sink of N2O, and consequently its atmospheric lifetime. N2O is a potent greenhouse gas with a global warming potential of currently approximately 300 CO2-equivalents in a 100-year perspective. A faster decay in atmospheric N2O mixing ratios, i.e. a decreased atmospheric lifetime of N2O, will also reduce its global warming potential. In order to assess the impact of climate change on atmospheric circulation and implied effects on the distribution and lifetime of atmospheric N2O, we apply the Max Planck Institute Earth System Model, MPI-ESM. MPI-ESM consists of the atmospheric general circulation model ECHAM, the land surface model JSBACH, and MPIOM/HAMOCC representing ocean circulation and ocean biogeochemistry. Prognostic atmospheric N2O concentrations in MPI-ESM are determined by land N2O emissions, ocean-atmosphere N2O exchange and atmospheric tracer transport. As stratospheric chemistry is not explicitly represented in MPI-ESM, stratospheric decay rates of N2O are prescribed from a MACC MOZART simulation. Increasing surface temperatures and CO2 concentrations in the stratosphere impact atmospheric circulation differently. Thus, we conduct a series of transient runs with the atmospheric model of MPI-ESM to isolate different factors governing a shift in atmospheric circulation. From those transient

  11. The effects of deoxynivalenol-contaminated corn dried distillers grains with solubles in nursery pig diets and potential for mitigation by commercially available feed additives.

    Science.gov (United States)

    Frobose, H L; Fruge, E D; Tokach, M D; Hansen, E L; DeRouchey, J M; Dritz, S S; Goodband, R D; Nelssen, J L

    2015-03-01

    Four experiments were conducted to investigate the effects of deoxynivalenol (DON) from naturally contaminated dried distillers grains with solubles (DDGS) and the efficacy of feed additives in nursery pig diets. In Exp. 1, 180 pigs (10.3 ± 0.2 kg BW) were fed 1 of 5 diets for 21 d. Diets were 1) Positive Control (PC; clay, and 5) NC + 0.25% Defusion Plus (Cargill Animal Nutrition, Minneapolis, MN). Pigs fed the NC diet had poorer ( clay had reduced ADG ( pelleted. Pigs fed the NC had decreased ( Pelleting improved ( pellet form: 1) PC (pellet form. No interactions were observed between pelleting and Defusion. Pigs fed the NC had decreased ( pelleting improved ( pelleting can help overcome some of the negative effects of DON, whereas other feed additives and additional nutrients do not.

  12. The Role of Grain Boundary Energy on Grain Boundary Complexion Transitions

    Energy Technology Data Exchange (ETDEWEB)

    Bojarski, Stephanie A. [Carnegie Mellon Univ., Pittsburgh, PA (United States). Dept. of Materials Science and Engineering; Rohrer, Gregory S. [Carnegie Mellon Univ., Pittsburgh, PA (United States). Dept. of Materials Science and Engineering

    2014-09-01

    Grain boundary complexions are distinct equilibrium structures and compositions of a grain boundary and complexion transformations are transition from a metastable to an equilibrium complexion at a specific thermodynamic and geometric conditions. Previous work indicates that, in the case of doped alumina, a complexion transition that increased the mobility of transformed boundaries and resulted in abnormal grain growth also caused a decrease in the mean relative grain boundary energy as well as an increase in the anisotropy of the grain boundary character distribution (GBCD). The current work will investigate the hypothesis that the rates of complexion transitions that result in abnormal grain growth (AGG) depend on grain boundary character and energy. Furthermore, the current work expands upon this understanding and tests the hypothesis that it is possible to control when and where a complexion transition occurs by controlling the local grain boundary energy distribution.

  13. MIXTURES » .

    African Journals Online (AJOL)

    fed broilers was attributed to deficiency of thiamine and vitamin K. B was r mmended followed ..... Fish Meal 4.00 4.00 4.00 3.00 3.00 3.00. Brewer's Dry Grain ... PremixZ -_Vitamin A lZSOiu, Vitamin D3 275iu, Vitamin E 1.50iu,. Biotin 0.005mg ...

  14. Grain growth and experimental deformation of fine-grained ice aggregates

    Science.gov (United States)

    Diebold, Sabrina; de Bresser, Hans; Spiers, Chris; Durham, William B.; Stern, Laura

    2010-05-01

    Ice is one of the most abundant materials in our solar system. It is the principal constituent of most of the moons of the outer solar system. Thus, the flow behavior of ice is of great interest when studying geodynamic processes on icy moons. Grain growth is an elementary process that is assumed to be important in the ice sheet layering of planetary moons, where temperatures 100-273 K exist. We concentrate on the questions to what extent grain growth may influence the evolution of strength of deforming ice and if the grain growth process is independent or dependent of deformation. The answers to these questions will help us to quantitatively test the hypothesis that the progressive evolution of the grain (crystal) size distribution of deforming and recrystallizing ice directly affects its rheological behaviour in terms of composite grain-size-sensitive (GSS) and grain-size-insensitive (GSI) creep, and that this might, after time, result in a steady state balance between mechanisms of GSS and GSI creep. We performed static grain growth experiments at different temperatures and a pressure (P) of 1 atm, and deformation experiments at P = 30-100 MPa starting in the GSS-creep field. The starting material ice Ih has a grain size growth tests a Hart Scientific temperature bath was filled with d-Limonene as cooling medium. The ice specimens were put into sealed alumina cylinders. For the grain growth tests, temperatures (T) between 213 K and 268 K were chosen. The durations of these tests varied between one day and two weeks. For the deformation experiments, temperatures of > 170 K and strain rates between 10-8 s-1 and 10-4 s-1 were chosen. Grain sizes, grain size distributions and grain topologies were measured by cryogenic SEM and image analysis techniques. We found clear evidence of grain growth and a significantly T-dependent variation of grain size distributions. The observations allow us to calibrate values for the grain size exponent n and the activation energy Q

  15. Development of an Acetate-Fed or Sugar-Fed Microbial Power Generator for Military Bases

    Science.gov (United States)

    2011-01-01

    Sucrose • Acetate Propionate • Hydrogen gas • Lactate • Formate • !so-butyrate Unknown fbiomass -’-soluble products ) KM. 80 60 I" 20 DavO...lactate and acetate as fermentation products and is ready for test in the MFC modules. 1. Anode materials We compared the performances of acetate-fed...microbial electrolysis cells (MECs) with graphite rods and stainless steel meshes as anodes to select the optimum material for use in MFC modules to

  16. Gene expression profiles and physiological data from mice fed resveratrol-enriched rice DJ526

    Science.gov (United States)

    Chung, Hea-Jong; Lee, Heui-Kwan; Kim, Hyeon-Jin; Baek, So-Hyeon; Hong, Seong-Tshool

    2016-01-01

    The molecular mechanism underlying lifespan extension by resveratrol remains widely discussed. To help study this mechanism, we previously created resveratrol-enriched rice, DJ526, by transferring the resveratrol biosynthesis gene into Dongjin rice. DJ526 accumulates 1.4–1.9 μg g−1 of resveratrol in its grain and can ameliorates age-related deterioration in mice, as compared to control animals, based on assessments of motor coordination, physical strength and cutaneous tissue aging. Here, we present raw data sets, deposited in public repositories, from microarray analysis and physiological data of mice fed with DJ526 and Dongjin rice and treated with resveratrol. We also provide a method to analyze blood serum at micron levels. These data sets may help other researchers find new clues regarding the etiology of the anti-aging process and signaling pathways induced by resveratrol, rice, or DJ526. PMID:27996975

  17. Grain boundary corrosion of copper canister material

    Energy Technology Data Exchange (ETDEWEB)

    Fennell, P.A.H.; Graham, A.J.; Smart, N.R.; Sofield, C.J. [AEA Technology plc, Harwell (United Kingdom)

    2001-03-01

    The proposed design for a final repository for spent fuel and other long-lived residues in Sweden is based on the multi-barrier principle. The waste will be encapsulated in sealed cylindrical canisters, which will then be placed in granite bedrock and surrounded by compacted bentonite clay. The canister design is based on a thick cast inner container fitted inside a corrosion-resistant copper canister. During fabrication of the outer copper canisters there will be some unavoidable grain growth in the welded areas. As grains grow they will tend to concentrate impurities within the copper at the new grain boundaries. The work described in this report was undertaken to determine whether there is any possibility of enhanced corrosion at grain boundaries within the copper canister. The potential for grain boundary corrosion was investigated by exposing copper specimens, which had undergone different heat treatments and hence had different grain sizes, to aerated artificial bentonite-equilibrated groundwater with two concentrations of chloride, for increasing periods of time. The degree of grain boundary corrosion was determined by atomic force microscopy (AFM) and optical microscopy. AFM showed no increase in grain boundary 'ditching' for low chloride groundwater. In high chloride groundwater the surface was covered uniformly with a fine-grained oxide. No increases in oxide thickness were observed. No significant grain boundary attack was observed using optical microscopy either. The work suggests that in aerated artificial groundwaters containing chloride ions, grain boundary corrosion of copper is unlikely to adversely affect SKB's copper canisters.

  18. Grain Unloading of Arsenic Species in Rice

    Energy Technology Data Exchange (ETDEWEB)

    Carey, Anne-Marie; Scheckel, Kirk G.; Lombi, Enzo; Newville, Matt; Choi, Yongseong; Norton, Gareth J.; Charnock, John M.; Feldmann, Joerg; Price, Adam H.; Meharg, Andrew A. (EPA); (U. South Australia); (Manchester); (Aberdeen); (UC)

    2010-01-11

    Rice (Oryza sativa) is the staple food for over half the world's population yet may represent a significant dietary source of inorganic arsenic (As), a nonthreshold, class 1 human carcinogen. Rice grain As is dominated by the inorganic species, and the organic species dimethylarsinic acid (DMA). To investigate how As species are unloaded into grain rice, panicles were excised during grain filling and hydroponically pulsed with arsenite, arsenate, glutathione-complexed As, or DMA. Total As concentrations in flag leaf, grain, and husk, were quantified by inductively coupled plasma mass spectroscopy and As speciation in the fresh grain was determined by x-ray absorption near-edge spectroscopy. The roles of phloem and xylem transport were investigated by applying a {+-} stem-girdling treatment to a second set of panicles, limiting phloem transport to the grain in panicles pulsed with arsenite or DMA. The results demonstrate that DMA is translocated to the rice grain with over an order magnitude greater efficiency than inorganic species and is more mobile than arsenite in both the phloem and the xylem. Phloem transport accounted for 90% of arsenite, and 55% of DMA, transport to the grain. Synchrotron x-ray fluorescence mapping and fluorescence microtomography revealed marked differences in the pattern of As unloading into the grain between DMA and arsenite-challenged grain. Arsenite was retained in the ovular vascular trace and DMA dispersed throughout the external grain parts and into the endosperm. This study also demonstrates that DMA speciation is altered in planta, potentially through complexation with thiols.

  19. Establishment of Grain Farmers' Supply Response Model and Empirical Analysis under Minimum Grain Purchase Price Policy

    OpenAIRE

    Zhang, Shuang

    2012-01-01

    Based on farmers' supply behavior theory and price expectations theory, this paper establishes grain farmers' supply response model of two major grain varieties (early indica rice and mixed wheat) in the major producing areas, to test whether the minimum grain purchase price policy can have price-oriented effect on grain production and supply in the major producing areas. Empirical analysis shows that the minimum purchase price published annually by the government has significant positive imp...

  20. Superior grains determined by grain weight are not fully correlated with the lfowering order in rice

    Institute of Scientific and Technical Information of China (English)

    PENG Ting; ZHAO Quan-zhi; L Qiang; ZHAO Ya-fan; SUN Hong-zheng; HAN Ying-chun; DU Yan-xiu; ZHANG Jing; LI Jun-zhou; WANG Lin-lin

    2015-01-01

    Rice panicles are composed of many branches with two types of extreme grains, the superior and the inferior. Traditional y, it has been wel accepted that earlier lfowers result in superior grains and late lfowers generate inferior grains. However, these correlations have never been strictly examined in practice. In order to determine the accurate relationship between superior and inferior grains and the lfowering order, we localized al the seeds in a panicle in four distinct rice species and systematical y documented the rice lfowering order, lfower locations and the ifnal grain weight for their relationships. Our results demonstrated that the grain weight is more heavily determined by the position of the seeds than by the lfowering order. Despite earlier lfowering has a positive correlation with the grain weight in general, grains from lfowers blooming on the second day after anthesis general y gained the highest weight. This suggests earlier lfowers may not result in superior grains. Therefore, we concluded that superior and inferior grains, commonly determined by grain weight, are not ful y cor-related with the lfowering order in rice. Fol owing the order of the grain weight, the superior grains are general y localized at the middle parts of the primary branches, whereas inferior grains were mainly on the last two secondary branches of the lower half part of the panicle. In addition, the weight of inferior grains were affected by spikelet thinning and spraying with exogenous plant growth regulators, indicating that physiological incompetence might be the major reason for the occurrence of the inferior grains.

  1. Plagioclase-Rich Itokawa Grains: Space Weathering, Exposure Ages, and Comparison to Lunar Soil Grains

    Science.gov (United States)

    Keller, L. P.; Berge, E.

    2017-01-01

    Regolith grains returned by the Hayabusa mission to asteroid 25143 Itokawa provide the only samples currently available to study the interaction of chondritic asteroidal material with the space weathering environment. Several studies have documented the surface alterations observed on the regolith grains, but most of these studies involved olivine because of its abundance. Here we focus on the rarer Itokawa plagioclase grains, in order to allow comparisons between Itokawa and lunar soil plagioclase grains for which an extensive data set exists.

  2. Evaluation of triticale dried distillers grains with solubles as a substitute for barley grain and barley silage in feedlot finishing diets.

    Science.gov (United States)

    Wierenga, K T; McAllister, T A; Gibb, D J; Chaves, A V; Okine, E K; Beauchemin, K A; Oba, M

    2010-09-01

    The objective of this study was to assess the value of triticale dried distillers grains with solubles (DDGS) as a replacement for barley silage in addition to a portion of the dry-rolled barley (DRB) in a grain-based feedlot finishing diet. The trial used 160 crossbred yearling steers: 144 noncannulated (478 +/- 84 kg) in a complete randomized design, and 16 ruminally cannulated (494 +/- 50 kg) in a replicated 4 x 4 Latin square design. The noncannulated steers were assigned to 8 standard pens (10 per pen) and 8 pens equipped with the GrowSafe system (GrowSafe Systems Ltd., Airdrie, Alberta, Canada; 8 per pen). The cannulated steers were placed (2 per pen) in the 8 GrowSafe pens and moved between pens at 28-d intervals. Each of 4 experimental diets was fed in 2 standard and 2 GrowSafe pens. The diets contained (DM basis) 1) 85% DRB and 10% barley silage (CON); 2) 65% DRB, 20% triticale DDGS, and 10% barley silage (D-10S), 3) 65% DRB, 25% triticale DDGS, and 5% barley silage, and 4) 65% DRB, 30% triticale DDGS, and no barley silage. Supplement (5% of dietary DM) was included in all diets. Ruminal pH was measured over four 7-d periods using indwelling electrodes. Replacing barley silage with triticale DDGS linearly decreased mean ruminal pH (P = 0.006), linearly increased duration (P = 0.006 and P = 0.01) and area under the curve (P = 0.02 and P = 0.05) below pH 5.5 and 5.2, and linearly increased the frequency of subacute (P = 0.005) and acute (P = 0.05) bouts of ruminal acidosis. Variation in mean ruminal pH decreased (P = 0.008) in steers fed D-10S compared with CON. Similarly, variation in DMI was less for steers fed triticale DDGS compared with CON. Steers fed D-10S tended to have greater DMI (P = 0.08) but similar ADG and G:F compared with CON steers. Replacing barley silage with triticale DDGS tended to linearly decrease DMI (P = 0.10) and increase (P = 0.06) G:F. Compared with CON, steers fed D-10S tended to have greater backfat thickness (P = 0.10) and

  3. Linear grain growth kinetics and rotation in nanocrystalline Ni

    OpenAIRE

    Farkas, Diana; Mohanty, S.; Monk, J.

    2007-01-01

    We report three-dimensional atomistic molecular dynamics studies of grain growth kinetics in nanocrystalline Ni. The results show the grain size increasing linearly with time, contrary to the square root of the time kinetics observed in coarse-grained structures. The average grain boundary energy per unit area decreases simultaneously with the decrease in total grain boundary area associated with grain growth. The average mobility of the boundaries increases as the grain size increases. The r...

  4. Stabilisation of the grain market by the flexible use of grain for bioethanol

    NARCIS (Netherlands)

    Helming, J.F.M.; Pronk, A.; Woltjer, I.

    2010-01-01

    This report reviews whether the grain market and grain price can be stabilised by the variation of the use of grain in the EU-27's production of bioethanol. The time horizon of this study is 2020, whereby account is taken of the minimum 10% obligation for biofuel use in the EU-27. An economic comput

  5. Stabilisation of the grain market by the flexible use of grain for bioethanol

    NARCIS (Netherlands)

    Helming, J.F.M.; Pronk, A.; Woltjer, I.

    2010-01-01

    This report reviews whether the grain market and grain price can be stabilised by the variation of the use of grain in the EU-27's production of bioethanol. The time horizon of this study is 2020, whereby account is taken of the minimum 10% obligation for biofuel use in the EU-27. An economic

  6. Irradiation-induced grain growth and defect evolution in nanocrystalline zirconia with doped grain boundaries.

    Science.gov (United States)

    Dey, Sanchita; Mardinly, John; Wang, Yongqiang; Valdez, James A; Holesinger, Terry G; Uberuaga, Blas P; Ditto, Jeff J; Drazin, John W; Castro, Ricardo H R

    2016-06-22

    Grain boundaries are effective sinks for radiation-induced defects, ultimately impacting the radiation tolerance of nanocrystalline materials (dense materials with nanosized grains) against net defect accumulation. However, irradiation-induced grain growth leads to grain boundary area decrease, shortening potential benefits of nanostructures. A possible approach to mitigate this is the introduction of dopants to target a decrease in grain boundary mobility or a reduction in grain boundary energy to eliminate driving forces for grain growth (using similar strategies as to control thermal growth). Here we tested this concept in nanocrystalline zirconia doped with lanthanum. Although the dopant is observed to segregate to the grain boundaries, causing grain boundary energy decrease and promoting dragging forces for thermally activated boundary movement, irradiation induced grain growth could not be avoided under heavy ion irradiation, suggesting a different growth mechanism as compared to thermal growth. Furthermore, it is apparent that reducing the grain boundary energy reduced the effectiveness of the grain boundary as sinks, and the number of defects in the doped material is higher than in undoped (La-free) YSZ.

  7. Probiotic Properties of Lactobacillus Strains Isolated from Tibetan Kefir Grains

    Science.gov (United States)

    Zheng, Yongchen; Lu, Yingli; Wang, Jinfeng; Yang, Longfei; Pan, Chenyu; Huang, Ying

    2013-01-01

    The objective of this study was to evaluate the functional properties of lactic acid bacteria (LAB) isolated from Tibetan kefir grains. Three Lactobacillus isolates identified as Lactobacillus acidophilus LA15, Lactobacillus plantarum B23 and Lactobacillus kefiri D17 that showed resistance to acid and bile salts were selected for further evaluation of their probiotic properties. The 3 selected strains expressed high in vitro adherence to Caco-2 cells. They were sensitive to gentamicin, erythromycin and chloramphenicol and resistant to vancomycin with MIC values of 26 µg/ml. All 3 strains showed potential bile salt hydrolase (BSH) activity, cholesterol assimilation and cholesterol co-precipitation ability. Additionally, the potential effect of these strains on plasma cholesterol levels was evaluated in Sprague-Dawley (SD) rats. Rats in 4 treatment groups were fed the following experimental diets for 4 weeks: a high-cholesterol diet, a high-cholesterol diet plus LA15, a high-cholesterol diet plus B23 or a high-cholesterol diet plus D17. The total cholesterol, triglyceride and low-density lipoprotein cholesterol levels in the serum were significantly (P<0.05) decreased in the LAB-treated rats compared with rats fed a high-cholesterol diet without LAB supplementation. The high-density lipoprotein cholesterol levels in groups B23 and D17 were significantly (P<0.05) higher than those in the control and LA15 groups. Additionally, both fecal cholesterol and bile acid levels were significantly (P<0.05) increased after LAB administration. Fecal lactobacilli counts were significantly (P<0.05) higher in the LAB treatment groups than in the control groups. Furthermore, the 3 strains were detected in the rat small intestine, colon and feces during the feeding trial. The bacteria levels remained high even after the LAB administration had been stopped for 2 weeks. These results suggest that these strains may be used in the future as probiotic starter cultures for manufacturing

  8. Temperature fluctuations of interstellar dust grains

    CERN Document Server

    Horn, Kobi; Biham, Ofer

    2007-01-01

    The temperatures of interstellar dust grains are analyzed using stochastic simulations, taking into account the grain composition and size and the discreteness of the photon flux. [...] The distribution of grain temperatures is calculated for a broad range of grain sizes and for different intensities of the interstellar radiation field, relevant to diffuse clouds and to PDRs. The dependence of the average grain temperature on its size is shown for different irradiation intensities. It is found that the average temperatures of grains with radii smaller than about 0.02 $\\mu$m are reduced due to the fluctuations. The average temperatures of grains of radii larger than about 0.35 $\\mu$m are also slightly reduced due to their more efficient emission of infrared radiation, particularly when exposed to high irradiation intensities. The average temperatures of silicate and carbonaceous grains are found to depend on the radiation field intensity X_MMP according to ~X_MMP^gamma, where the exponent gamma depends on the...

  9. Grain transport mechanics in shallow overland flow

    Science.gov (United States)

    A physical model based on continuum multiphase flow is described to represent saltating transport of grains in shallow overland flow. The two phase continuum flow of water and sediment considers coupled St.Venant type equations. The interactive cumulative effect of grains is incorporated by a disper...

  10. Grain transport mechanics in shallow flow

    Science.gov (United States)

    A physical model based on continuum multiphase flow is described to represent saltating transport of grains in shallow overland flows. The two-phase continuum flow of water and sediment considers coupled St.Venant type equations. The interactive cumulative effect of grains is incorporated by a dispe...

  11. Grain boundaries in high temperature superconductors

    NARCIS (Netherlands)

    Hilgenkamp, Johannes W.M.; Mannhart, J.

    2002-01-01

    Since the first days of high-Tc superconductivity, the materials science and the physics of grain boundaries in superconducting compounds have developed into fascinating fields of research. Unique electronic properties, different from those of the grain boundaries in conventional metallic

  12. Reducing grain storage losses in developing countries

    Science.gov (United States)

    We investigated the use of insecticide-treated material and modified atmosphere storage for reducing insect damage in stored maize. Results showed that insecticide treated netting and insecticide treated seed bags protected grain from insect damage for up to nine months if the grain was free from i...

  13. SEGMENTATION AND GRAIN SIZE OF CERAMICS

    Directory of Open Access Journals (Sweden)

    Xavier Arnould

    2011-05-01

    Full Text Available This paper presents some methods to automatically extract the grain boundaries of materials in order to develop an automatic method to determine the grain size and morphological parameters of ceramic materials. Results are presented in the case of sintered cerine (CeO2 materials.

  14. Insect Population Dynamics in Commercial Grain Elevators

    Science.gov (United States)

    Data were collected in 1998-2002 from wheat stored in commercial grain elevators in south-central Kansas. Storage bins at these elevators had concrete walls and were typically 6-9 m in diameter and 30-35 m tall. A vacuum-probe sampler was used to collect ten 3-kg grain samples in the top 12 m of the...

  15. Grain boundaries in high-Tc superconductors

    NARCIS (Netherlands)

    Hilgenkamp, H.; Mannhart, J.

    2002-01-01

    Since the first days of high-Tc superconductivity, the materials science and the physics of grain boundaries in superconducting compounds have developed into fascinating fields of research. Unique electronic properties, different from those of the grain boundaries in conventional metallic supercondu

  16. Topological Transformation during Normal Grain Growth

    Institute of Scientific and Technical Information of China (English)

    Chaogang LOU; Michael A.Player

    2004-01-01

    This paper investigates topological transformation during normal grain growth by carrying out a computer vertex simulation.Results show that topological correlation agrees with the models proposed by Blanc et al. and Weaire. Topological transformation occurs more often on grains with some topological classes instead of equal probability on each boundary. This can be qualitatively explained by topological correlation.

  17. Quality of royal jelly produced by Africanized honeybees fed a supplemented diet

    Directory of Open Access Journals (Sweden)

    Maria Josiane Sereia

    2013-06-01

    Full Text Available This study was carried out to evaluate the effect of artificial supplements prepared with soybean protein isolate, brewer's yeast, mixture of soybean protein isolate with brewer's yeast, linseed oil, palm oil, and a mixture of linseed oil with palm oil on the physicochemical and microbiological composition of royal jelly produced by Africanized honey bee colonies. Considering these results, providing supplements for Africanized honeybee colonies subjected to royal jelly production can help and strengthen the technological development of the Brazilian beekeeping industry increasing its consumption in the national market. This research presents values of royal jelly a little different from those established by the Brazilian legislation. This fact shows that is important to discuss or change the official method for royal jelly analysis. The characterization of physicochemical and microbiological parameters is important in order to standardize fresh, frozen, and lyophilized royal jelly produced by Africanized honeybees.

  18. Growth indices and economic implications of weaned rabbits fed ...

    African Journals Online (AJOL)

    Growth indices and economic implications of weaned rabbits fed Leucaena ... the 13.47g weight gain on rabbits fed the control diet but statistically (P<0.05) lower ... economic efficiency of 0.35 and 194.44%, respectively indicated the optimal ...

  19. Digital Implementation of Two Inductor Boost Converter Fed DC Drive

    Directory of Open Access Journals (Sweden)

    G. Kishor

    2011-01-01

    Full Text Available The study deals w ith simulation and implementation of two inductor boost converter fed DC drive. The two inductor boost converter fed DC drive is simulated and implemented. The circuit has advantages like higher output voltage and improved power factor. The laboratory model is implemented and the experimental results are obtained. The experimental results were compared w ith the simulation results.

  20. Manure total nitrogen flux from condensed tannin fed beef cattle

    Science.gov (United States)

    A study was conducted to determine the effects of three levels of condensed tannins fed to 27 beef feedyard steers on total nitrogen (N) flux from manure. Condensed tannins were fed at rates of 0, 0.5, and 1 percent of the daily ration on a dry matter basis. Manure and urine were collected over two ...