WorldWideScience

Sample records for fast red kb

  1. A 660-Kb Deletion with Antagonistic Effects on Fertility and Milk Production Segregates at High Frequency in Nordic Red Cattle

    DEFF Research Database (Denmark)

    Kadri, Naveen Kumar; Sahana, Goutam; Charlier, Carole

    The spectacular increase in productivity of dairy cattle has been accompanied by a decline in fertility. It is assumed that this reduction is due to the negative energy balance of high producing cows. We herein describe the dissection of a fertility QTL in Nordic Red cattle to a 660-Kb deletion...... on BTa 12. The deletion encompasses four genes and affects fertility by causing death in homozygous embryos. Despite its deleterious effect 32% of the Finnish Ayrshire animals carry the deletion. We show that the deletion has a positive effect on milk yield and that the balancing selection maintains...... it at a higher frequency. Our study demonstrates the Embryonic lethal mutations are partly responsible for decline in fertility and that associated positive effects on milk yield may partly account for the negative genetic correlation....

  2. A 660-Kb deletion with antagonistic effects on fertility and milk production segregates at high frequency in Nordic Red cattle

    DEFF Research Database (Denmark)

    Kadri, Naveen Kumar; Sahana, Goutam; Charlier, Carole

    2014-01-01

    In dairy cattle, the widespread use of artificial insemination has resulted in increased selection intensity, which has led to spectacular increase in productivity. However, cow fertility has concomitantly severely declined. It is generally assumed that this reduction is primarily due to the nega......In dairy cattle, the widespread use of artificial insemination has resulted in increased selection intensity, which has led to spectacular increase in productivity. However, cow fertility has concomitantly severely declined. It is generally assumed that this reduction is primarily due...... to the negative energy balance of high-producing cows at the peak of lactation. We herein describe the fine-mapping of a major fertility QTL in Nordic Red cattle, and identify a 660-kb deletion encompassing four genes as the causative variant. We show that the deletion is a recessive embryonically lethal mutation....... This probably results from the loss of RNASEH2B, which is known to cause embryonic death in mice. Despite its dramatic effect on fertility, 13%, 23% and 32% of the animals carry the deletion in Danish, Swedish and Finnish Red Cattle, respectively. To explain this, we searched for favorable effects on other...

  3. Star-planet interactions: II. Is planet engulfment the origin of fast rotating red giants?

    CERN Document Server

    Privitera, Giovanni; Eggenberger, Patrick; Vidotto, Aline A; Villaver, Eva; Bianda, Michele

    2016-01-01

    Context. Fast rotating red giants in the upper part of the red giant branch have surface velocities that cannot be explained by single star evolution. Aims. We check whether tides between a star and a planet followed by planet engulfment can indeed accelerate the surface rotation of red giants for a sufficient long time in order to produce these fast rotating red giants. Methods. Using rotating stellar models, accounting for the redistribution of the angular momentum inside the star by different transport mechanisms, for the exchanges of angular momentum between the planet orbit and the star before the engulfment and for the deposition of angular momentum inside the star at the engulfment, we study how the surface rotation velocity at the stellar surface evolves. Results. We show that the surface velocities reached at the end of the orbital decay due to tidal forces and planet engulfment can be similar to values observed for fast rotating red giants. This surface velocity then decreases when the star evolves ...

  4. Spectrophotometric Method for the Determination of Some Drugs Using Fast Red B Salt

    Directory of Open Access Journals (Sweden)

    Afaf Abul Khier

    2008-01-01

    Full Text Available A simple spectrophorometric method for the determination of secnidazole, niclosamide, nifuroxazide and sulphasalzine is described. The method is based on reduction of the nitro group present in secnidazole and niclosamide molecule using zinc powder and dilute hydrochloric acid followed by reaction with fast red B salt in presence of ammonium chloride and sodium hydroxide, while in case of nifuroxazide and sulphasalazine the reaction takes place directly without any prior reduction between the phenolic group present in each drug and fast red B salt in presence of sodium hydroxide. Beer's law is valid in the concentration ranges 2.5-15, 1.25-10, 2.5-15, and 2.5-13.75 μg.mL-1 for secnidazole, niclosamide, nifuroxazide and sulphasalazine respectively. The proposed method is applied successfully for the estimation of the mentioned drugs either in pure form or in their pharmaceutical formulations.

  5. Star-planet interactions. II. Is planet engulfment the origin of fast rotating red giants?

    Science.gov (United States)

    Privitera, Giovanni; Meynet, Georges; Eggenberger, Patrick; Vidotto, Aline A.; Villaver, Eva; Bianda, Michele

    2016-10-01

    Context. Fast rotating red giants in the upper part of the red giant branch have surface velocities that cannot be explained by single star evolution. Aims: We check whether tides between a star and a planet followed by planet engulfment can indeed accelerate the surface rotation of red giants for a sufficiently long time to produce these fast rotating red giants. Methods: We studied how the surface rotation velocity at the stellar surface evolves using rotating stellar models, accounting for the redistribution of the angular momentum inside the star by different transport mechanisms, the exchanges of angular momentum between the planet orbit and the star before the engulfment, and for the deposition of angular momentum inside the star at the engulfment. We considered different situations with masses of stars in the range between 1.5 and 2.5 M⊙, masses of the planets between 1 and 15 MJ (Jupiter mass), and initial semimajor axis between 0.5 and 1.5 au. The metallicity Z for our stellar models is 0.02. Results: We show that the surface velocities reached at the end of the orbital decay due to tidal forces and planet engulfment can be similar to values observed for fast rotating red giants. This surface velocity then decreases when the star evolves along the red giant branch but at a sufficiently slow pace to allowing stars to be detected with such a high velocity. More quantitatively, star-planet interaction can produce a rapid acceleration of the surface of the star, above values equal to 8 km s-1, for periods lasting up to more than 30% the red giant branch phase. As found already by previous works, the changes of the surface carbon isotopic ratios produced by the dilution of the planetary material into the convective envelope is modest. The increase of the lithium abundance due to this effect might be much more important, however lithium may be affected by many different, still uncertain, processes. Thus any lithium measurement can hardly be taken as a support

  6. Lipolytic response of adipose tissue and metabolic adaptations to long periods of fasting in red tilapia (Oreochromis sp., Teleostei: Cichlidae

    Directory of Open Access Journals (Sweden)

    WALTER DIAS JUNIOR

    2016-01-01

    Full Text Available ABSTRACT Adaptive changes of carbohydrate and lipid metabolism induced by 7, 15, 30, 60, 90, 150 and 200 days of fasting were investigated in red tilapia (Oreochromis sp.. Plasma glucose, lactate and free fatty acids (FFA levels, liver and muscle glycogen and total lipid contents and rates of FFA release from mesenteric adipose tissue (MAT were measured. Plasma glucose levels showed significant differences only after 90 days of fasting, when glycemia was 34% lower (50±5mg.dL-1 than fed fish values (74±1mg.dL-1, remaining relatively constant until 200 days of fasting. The content of liver glycogen ("15% in fed tilapia fell 40% in 7 days of food deprivation. In 60, 90 and 150 days of fasting, plasma FFA levels increased 49%, 64% and 90%, respectively, compared to fed fish values. In agreement with the increase in plasma FFA, fasting induced a clear increase in lipolytic activity of MAT incubated in vitro. Addition of isobutylmethylxanthine (cAMP-phosphodiesterase inhibitor and isoproterenol (non selective beta adrenergic agonist to the incubation medium induced a reduction of lipolysis in fasted fish, differently to what was observed in mammal adipose tissue. This study allowed a physiological assessment of red tilapia response to starvation.

  7. Fast and sensitive detection of ochratoxin A in red wine by nanoparticle-enhanced SPR.

    Science.gov (United States)

    Karczmarczyk, Aleksandra; Reiner-Rozman, Ciril; Hageneder, Simone; Dubiak-Szepietowska, Monika; Dostálek, Jakub; Feller, Karl-Heinz

    2016-09-21

    Herein, we present a fast and sensitive biosensor for detection of Ochratoxin A (OTA) in a red wine that utilizes gold nanoparticle-enhanced surface plasmon resonance (SPR). By combining an indirect competitive inhibition immunoassay and signal enhancement by secondary antibodies conjugated with gold nanoparticles (AuNPs), highly sensitive detection of low molecular weight compounds (such as OTA) was achieved. The reported biosensor allowed for OTA detection at concentrations as low as 0.75 ng mL(-1) and its limit of detection was improved by more than one order of magnitude to 0.068 ng mL(-1) by applying AuNPs as a signal enhancer. The study investigates the interplay of size of AuNPs and affinity of recognition elements affecting the efficiency of the signal amplification strategy based on AuNP. Furthermore, we observed that the presence of polyphenolic compounds in wine samples strongly interferes with the affinity binding on the surface. To overcome this limitation, a simple pre-treatment of the wine sample with the binding agent poly(vinylpyrrolidone) (PVP) was successfully applied.

  8. Application of adsorptive stripping voltammetry to the simultaneous determination of bismuth and copper in the presence of nuclear fast red.

    Science.gov (United States)

    Gholivand, M B; Romiani, A A

    2006-06-30

    A sensitive and selective method for the simultaneous determination of copper and bismuth by adsorptive stripping was developed using nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) as selective complexing agent onto hanging mercury drop electrode. In a single scan both metals gave peaks that were distinctly separated by 85 mV allowing their determination in the presence of each other. Optimal analytical conditions were found to be: nuclear fast red concentration of 80 microM, pH of 2.8 and adsorptive potential of -300 mV versus Ag/AgCl. With accumulation time of 180s the peaks currents are proportional to concentration of copper and bismuth over the 1-100 and 5-60 ng mL(-1) range with detection limits of 0.2 and 1.2 ng mL(-1), respectively. The procedure was applied to simultaneous determination of copper and bismuth in some real samples.

  9. Furiously Fast and Red: Sub-second Optical Flaring in V404 Cyg during the 2015 Outburst Peak

    CERN Document Server

    Gandhi, P; Hardy, L K; Dhillon, V S; Marsh, T R; Shaw, A W; Altamirano, D; Caballero-Garcia, M D; Casares, J; Casella, P; Castro-Tirado, A J; Charles, P A; Dallilar, Y; Eikenberry, S; Fender, R P; Hynes, R I; Knigge, C; Kuulkers, E; Mooley, K; Muñoz-Darias, T; Pahari, M; Rahoui, F; Russell, D M; Santisteban, J V Hernández; Shahbaz, T; Terndrup, D M; Tomsick, J; Walton, D J

    2016-01-01

    We present observations of rapid (sub-second) optical flux variability in V404 Cyg during its 2015 June outburst. Simultaneous three-band observations with the ULTRACAM fast imager on four nights show steep power spectra dominated by slow variations on ~100-1000s timescales. Near the peak of the outburst on June 26, a dramatic change occurs and additional, persistent sub-second optical flaring appears close in time to giant radio and X-ray flaring. The flares reach peak optical luminosities of ~few x 10^{36} erg/s. Some are unresolved down to a time resolution of 24 milliseconds. Whereas the fast flares are stronger in the red, the slow variations are bluer when brighter. The redder slopes, emitted power, and characteristic timescales of the fast flares can be explained as optically-thin synchrotron emission from a compact jet arising on size scales ~140-500 Gravitational radii (with a possible additional contribution by a thermal particle distribution). The origin of the slower variations is unclear. The opt...

  10. The Red Queen's race: running twice as fast or a slow sort of country?

    OpenAIRE

    Collinson, Timothy

    2014-01-01

    Way back in 1949 Isaac Asimov entitled one of his science fiction short stories ‘The Red Queen’s Race’ which was about using time travel to try to improve the world. The recent film All About Time finds Tim and his Dad trying to improve their lives with time travel. And who hasn’t wished for Hermione’s ‘time turner’ (Harry Potter and the Prisoner of Azkaban) which she used to fit in more study. Time travel would be great - we’d be able to get more done, we’d be able to improve our lives, the ...

  11. Fast computation of radiation pressure force exerted by multiple laser beams on red blood cell-like particles

    Science.gov (United States)

    Gou, Ming-Jiang; Yang, Ming-Lin; Sheng, Xin-Qing

    2016-10-01

    Mature red blood cells (RBC) do not contain huge complex nuclei and organelles, makes them can be approximately regarded as homogeneous medium particles. To compute the radiation pressure force (RPF) exerted by multiple laser beams on this kind of arbitrary shaped homogenous nano-particles, a fast electromagnetic optics method is demonstrated. In general, based on the Maxwell's equations, the matrix equation formed by the method of moment (MOM) has many right hand sides (RHS's) corresponding to the different laser beams. In order to accelerate computing the matrix equation, the algorithm conducts low-rank decomposition on the excitation matrix consisting of all RHS's to figure out the so-called skeleton laser beams by interpolative decomposition (ID). After the solutions corresponding to the skeletons are obtained, the desired responses can be reconstructed efficiently. Some numerical results are performed to validate the developed method.

  12. FAST

    DEFF Research Database (Denmark)

    Zuidmeer-Jongejan, Laurian; Fernandez-Rivas, Montserrat; Poulsen, Lars K.

    2012-01-01

    ABSTRACT: The FAST project (Food Allergy Specific Immunotherapy) aims at the development of safe and effective treatment of food allergies, targeting prevalent, persistent and severe allergy to fish and peach. Classical allergen-specific immunotherapy (SIT), using subcutaneous injections with aqu......ABSTRACT: The FAST project (Food Allergy Specific Immunotherapy) aims at the development of safe and effective treatment of food allergies, targeting prevalent, persistent and severe allergy to fish and peach. Classical allergen-specific immunotherapy (SIT), using subcutaneous injections...... with aqueous food extracts may be effective but has proven to be accompanied by too many anaphylactic side-effects. FAST aims to develop a safe alternative by replacing food extracts with hypoallergenic recombinant major allergens as the active ingredients of SIT. Both severe fish and peach allergy are caused...... in depth serological and cellular immune analyses will be performed, allowing identification of novel biomarkers for monitoring treatment efficacy. FAST aims at improving the quality of life of food allergic patients by providing a safe and effective treatment that will significantly lower their threshold...

  13. An advanced KB mirror pair for microfocusing

    CERN Document Server

    Ferme, J J

    2001-01-01

    A new range of micro-focusing mirrors based on KB pairs has been developed by SESO for Beamline Nanospectroscopy at the Elettra Storage Ring in Trieste, Italy. Both the focusing and the aspheric shape are adjustable with stepper motors. The goal of the beamline is to have a high photon density spot with a variable size in the experimental chamber over the whole soft X-ray range. The estimated dimension of the final spot should be smaller than 4 mu m sup 2 FWHM, with a photon density of the order of 10 sup 1 sup 3 photons/s mu m sup 2; this may be achieved only by accepting an angular divergence on these mirrors of between 5 and 10 mrad. This condition can be fulfilled only with elliptical (or plane elliptical) mirrors with very limited residual slope errors (below 1 mu rad RMS) that are able to correct even small focal distance errors.

  14. ANALISIS PELAYANAN KB MANDIRI WANITA USIA SUBUR BERDASARKAN STATUS EKONOMI

    Directory of Open Access Journals (Sweden)

    Selma Siahaan

    2013-08-01

    Full Text Available Latar belakang: Penggunaan metode keluarga berencana (KB oleh Wanita Usia Subur (WUS antara lain dipengaruhi oleh faktor sosial ekonomi. Maka, masalah biaya yang harus dibayar oleh WUS untuk memperoleh pelayanan KB perludi pahami guna keberhasilan program KB Nasional. Metode: Studi menggunakan data Riset Kesehatan Dasar (Riskesdas 2010, bertujuan untuk mengetahui biaya pelayanan KB yang dibayar oleh perempuan usia 10–59 tahun (WUS yang pernah kawin menurut metode KB berdasarkan status ekonomi yang bersangkutan. Analisis data menggunakan analisis deskriptif, analisis bivariat dengan uji statistik chi-square. Hasil: Alat/obat KB disposible terbanyak yang digunakan WUS dari semua golongan status ekonomi adalah metode KB suntik, sedangkan kondom terbanyak digunakan oleh WUS dengan status ekonomi atas banyak. Sekitar 50% WUS dengan status ekonomi (SE rendah membayar biaya pelayanan KB berkisar Rp.10.000–Rp.15.000. Sementara itu WUS dengan SE atas terbanyak membayar sekitar > Rp. 20.000. Terdapat korelasi signifikan antara metode KB dengan biaya dan status ekonomi. Kesimpulan: Metode suntik masih merupakan metode pilihan bagi WUS di Indonesia dan pemilihan metode KB oleh WUS berhubungan dengan status ekonomi mereka. Oleh karena itu promosi kesehatan perlu terus digalakkan agar program KB pemerintah dengan IUD dapat lebih dikenal dan dapat diterima masyarakat. Di samping itu, diperlukan kebijakan khusus (bantuan kepada WUS dengan status ekonomi rendah sehingga IUD dapat diperoleh secara gratis atau setidaknya dengan biaya yang terjangkau.

  15. Processing of red ceramic using a fast-firing cycle Processamento de cerâmica vermelha usando um ciclo de queima rápido

    Directory of Open Access Journals (Sweden)

    G. T. Saleiro

    2012-09-01

    Full Text Available This work reports on the processing of red ceramic for civil construction using fast-firing cycles. The firing cycle is an important variable in the processing of red ceramic materials, which contributes to a high consumption of energy. The red ceramic pieces were prepared by industrial extrusion and fired at firing temperatures varying from 700 ºC to 1100 ºC using different firing cycles (slow-firing cycle - 1º C/min and fast-firing cycle - 10 ºC/min and 20 °C/min. The technological properties (linear shrinkage, water absorption, apparent porosity, apparent density, and flexural strength as function of the firing temperature and firing cycle are investigated. The development of the microstructure was followed by SEM/SEI. The results showed that fast-firing red ceramics exhibits technological properties and microstructure comparable to conventionally fired red ceramics, resulting in great advantages in energy saving.Este trabalho descreve o processamento de cerâmica vermelha para construção civil usando ciclos de queima rápido. O ciclo de queima é uma variável importante no processamento de materiais de cerâmica vermelha, o qual contribui para um alto consumo de energia. As peças de cerâmica vermelha foram preparadas por extrusão industrial e queimadas nas temperaturas de queima variando de 700 ºC a 1100 ºC utilizando diferentes ciclos de queima (ciclo de queima lento - 1 ºC/min e ciclos de queima rápidos - 10 ºC/ min e 20 ºC/min. As propriedades tecnológicas (retração linear, absorção de água, porosidade aparente, massa específica aparente e tensão de ruptura à flexão em função da temperatura de queima e ciclo de queima são investigadas. O desenvolvimento da microestrutura foi avaliado por SEM/SEI. Os resultados mostraram que as peças de cerâmica vermelha obtidas via processo de queima rápida exibiram propriedades tecnológicas e microestrutura comparáveis àquelas convencionalmente obtidas via queima lenta

  16. Application of dispersive solid-phase extraction and ultra-fast liquid chromatography-tandem quadrupole mass spectrometry in food additive residue analysis of red wine.

    Science.gov (United States)

    Chen, Xiao-Hong; Zhao, Yong-Gang; Shen, Hao-Yu; Jin, Mi-Cong

    2012-11-09

    A novel and effective dispersive solid-phase extraction (dSPE) procedure with rapid magnetic separation using ethylenediamine-functionalized magnetic polymer as an adsorbent was developed. The new procedure had excellent clean-up ability for the selective removal of the matrix in red wine. An accurate, simple, and rapid analytical method using ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) for the simultaneous determination of nine food additives (i.e., acesulfame, saccharin, sodium cyclamate, aspartame, benzoic acid, sorbic acid, stevioside, dehydroacetic acid, and neotame) in red wine was also used and validated. Recoveries ranging from 78.5% to 99.2% with relative standard deviations ranging from 0.46% to 6.3% were obtained using the new method. All target compounds showed good linearities in the tested range with correlation coefficients (r) higher than 0.9993. The limits of quantification for the nine food additives were between 0.10 μg/L and 50.0 μg/L. The proposed dSPE-UFLC-MS/MS method was successfully applied in the food-safety risk monitoring of real red wine in Zhejiang Province, China. Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.

  17. FastStats: Sinus Conditions

    Science.gov (United States)

    ... please visit this page: About CDC.gov . FastStats Homepage Diseases and Conditions Anemia or Iron Deficiency Arthritis ... Statistics Tables for U.S. Adults: National Health Interview Survey, 2014, Table A-2 [PDF - 219 KB] Physician ...

  18. Photoconductivity and dielectric studies of potassium pentaborate crystal (KB5)

    Indian Academy of Sciences (India)

    V Joseph; S Gunasekaran; V Santhanam

    2003-06-01

    Single crystal of potassium pentaborate (KB5) has been grown by solution growth technique. FTIR and laser Raman measurements are carried out to make a qualitative analysis on KB5 crystal. Dielectric behaviour of the KB5 crystal has been studied in the microwave region using K-band microwave bench equipped with the Gunn Oscillator guided with rectangular wave-guide. To confirm the suitability of this crystal as electro optic device, its dielectric behaviour with the change of frequency has also been investigated. Photoconductivity studies were also carried out on this material. It was interesting to observe that the KB5 crystal exhibited negative photoconductivity.

  19. Different effects of growth hormone and fasting on the induction patterns of two hormone-sensitive lipase genes in red seabream Pagrus major.

    Science.gov (United States)

    Khieokhajonkhet, Anurak; Kaneko, Gen; Hirano, Yuki; Wang, Lu; Ushio, Hideki

    2016-09-15

    Growth hormone (GH) increases phosphorylation and mRNA levels of hormone-sensitive lipase (HSL) in the livers of some marine teleosts. The hepatic GH-HSL axis appears to play important roles in fasting-induced lipolysis. However, it is not known whether GH exerts similar effects on HSL in fish adipose tissues. Functional differentiation of two fish-specific HSL isoforms (HSL1 and HSL2) also remains unclear. The present study seeks to address two unanswered questions about fish lipolysis using red seabream (Pagrus major): (1) Does GH increase phosphorylation and mRNA levels of HSL in adipose tissue? (2) How do GH and fasting affect mRNA levels of two HSL isoform genes in the liver and adipose tissue? To this end, we first cloned HSL1 and HSL2 cDNAs and investigated their tissue distribution. Transcripts of both HSLs and HSL1 proteins were abundant in the visceral adipose tissue, gonads, and liver, suggesting the important role of HSL in adipose tissue lipolysis. HSL2 transcript levels were 20-65% those of HSL1 except in the skin, and HSL2 proteins were not detected by our in-house antisera. Ex vivo administration of GH increased HSL1 phosphorylation, non-esterified fatty acid (NEFA) release, and levels of HSL1 and HSL2 mRNA in both the liver and visceral adipose tissue. Hepatic HSL2 mRNA was particularly sensitive to GH administration and sometimes exceeded HSL1 mRNA levels with up to 13-fold induction. In contrast, fasting for 4 and 7d increased HSL1 mRNA levels, but had only marginal effects on HSL2 mRNA levels in both adipose tissue or liver. We concluded that GH would increase HSL mRNAs during adipose tissue lipolysis in red seabream; however, GH and fasting result in different induction ratio of two HSL isoform genes, suggesting that other hormone(s) also contributes to fasting-induced lipolysis. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Wear Fast, Die Young: More Worn Teeth and Shorter Lives in Iberian Compared to Scottish Red Deer.

    Directory of Open Access Journals (Sweden)

    F J Pérez-Barbería

    Full Text Available Teeth in Cervidae are permanent structures that are not replaceable or repairable; consequently their rate of wear, due to the grinding effect of food and dental attrition, affects their duration and can determine an animal's lifespan. Tooth wear is also a useful indicator of accumulative life energy investment in intake and mastication and their interactions with diet. Little is known regarding how natural and sexual selection operate on dental structures within a species in contrasting environments and how these relate to life history traits to explain differences in population rates of tooth wear and longevity. We hypothesised that populations under harsh environmental conditions should be selected for more hypsodont teeth while sexual selection may maintain similar sex differences within different populations. We investigated the patterns of tooth wear in males and females of Iberian red deer (Cervus elaphus hispanicus in Southern Spain and Scottish red deer (C. e. scoticus across Scotland, that occur in very different environments, using 10343 samples from legal hunting activities. We found higher rates of both incisor and molar wear in the Spanish compared to Scottish populations. However, Scottish red deer had larger incisors at emergence than Iberian red deer, whilst molars emerged at a similar size in both populations and sexes. Iberian and Scottish males had earlier tooth depletion than females, in support of a similar sexual selection process in both populations. However, whilst average lifespan for Iberian males was 4 years shorter than that for Iberian females and Scottish males, Scottish males only showed a reduction of 1 year in average lifespan with respect to Scottish females. More worn molars were associated with larger mandibles in both populations, suggesting that higher intake and/or greater investment in food comminution may have favoured increased body growth, before later loss of tooth efficiency due to severe wear. These

  1. Fast and efficient DNA-based method for winter diet analysis from stools of three cervids: moose, red deer, and roe deer.

    Science.gov (United States)

    Czernik, Marta; Taberlet, Pierre; Swisłocka, Magdalena; Czajkowska, Magdalena; Duda, Norbert; Ratkiewicz, Mirosław

    2013-01-01

    Effects of cervid browsing on timber production, especially during winter, lead to economic losses in forest management. The aim of this study was to present an efficient DNA-based method which allows qualitative assessment of the winter diet from stools of moose (Alces alces), roe deer (Capreolus capreolus), and red deer (Cervus elaphus). The preliminary results of the diet composition of the three cervids from Poland were also presented with a special emphasis on moose. The electropherograms of the chloroplast intron trnL (UAA) P6 loop amplification products using g (fluorescence-labeled) and h primers revealed differences in the length of PCR products among various plant species eaten by these herbivores. In addition, the usage of species-specific primers allowed unambiguous identification of different gymnosperms and angiosperms. The preliminary moose diet analysis, based on winter fecal samples from the entire range of moose occurrence in Poland, revealed the presence of 15 to 24 tree, shrub, and herbaceous species. This fast, cost-efficient, and simple method proved also to be reliable for the diet analysis of red deer and roe deer. It may be a valuable tool in forest and conservation management, as well as a way of enhancing ecological studies focusing on the impact of herbivores on the ecosystems and their possible food niche overlap.

  2. Improving thermal stability and light fastness of Acid Red 114 by incorporating its anions in a ZnAl-layered double hydroxides matrix

    Institute of Scientific and Technical Information of China (English)

    Haifeng Zhu; Liren Wang; Pinggui Tang; Yongjun Feng; Dianqing Li

    2012-01-01

    Incorporation of anions of Acid Red 114 dye (1,3-naphthalenedisulfonic acid,8-[2-[3,3'-dimethyl-4'-[2-[4-[[(4-methylphenyl)sulfonyl]oxy] phenyl]diazenyl] [1,1'-biphenyl]-4-yl]diazenyl]-7-hydroxy-,disodium salt) (denoted as NPDA) into ZnAl-layered double hydroxides (LDHs) has been carried out by an anionexchange method in an effort to improve their thermal stability and light fastness.After intercalation of NPDA anions,the interlayer distance of the LDHs increases from 0.87 to 2.18nm,confirming their incorporation into the interlayer galleries of the LDHs host.Infrared spectroscopy and thermogravimetric analysis revealed the presence of host-guest interactions between LDHs layers and NPDA anions.The thermal stability of NPDA and ZnAl-NPDA-LDHs was compared by thermogravimetric-differential thermal analysis,UV-visible spectroscopy and infrared spectroscopy.It was found that the thermal stability of NPDA anions was markedly improved by incorporation into the ZnAl-LDHs matrix,while the light fastness was also enhanced.

  3. Proposed Application of Fast Fourier Transform in Near Infra Red Based Non Invasive Blood Glucose Monitoring System

    Science.gov (United States)

    Jenie, R. P.; Iskandar, J.; Kurniawan, A.; Rustami, E.; Syafutra, H.; Nurdin, N. M.; Handoyo, T.; Prabowo, J.; Febryarto, R.; Rahayu, M. S. K.; Damayanthi, E.; Rimbawan; Sukandar, D.; Suryana, Y.; Irzaman; Alatas, H.

    2017-03-01

    Worldwide emergence of glycaemic status related health disorders, such as diabetes and metabolic syndrome, is growing in alarming rate. The objective was to propose new methods for non invasive blood glucose level measurement system, based on implementation of Fast Fourier Transform methods. This was an initial-lab-scale-research. Data on non invasive blood glucose measurement are referred from Scopus, Medline, and Google Scholar, from 2011 until 2016, and was used as design references, combined with in house verification. System was developed in modular fashion, based on aforementioned compiled references. Several preliminary tests to understand relationship between LED and photo-diode responses have been done. Several references were used as non invasive blood glucose measurement tools design basis. Solution is developed in modular fashion. we have proven different sensor responses to water and glucose. Human test for non invasive blood glucose level measurement system is needed.

  4. Strike fast, strike hard: the red-throated caracara exploits absconding behavior of social wasps during nest predation.

    Directory of Open Access Journals (Sweden)

    Sean McCann

    Full Text Available Red-throated Caracaras Ibycter americanus (Falconidae are specialist predators of social wasps in the Neotropics. It had been proposed that these caracaras possess chemical repellents that allow them to take the brood of wasp nests without being attacked by worker wasps. To determine how caracaras exploit nests of social wasps and whether chemical repellents facilitate predation, we: (1 video recorded the birds attacking wasp nests; (2 analyzed surface extracts of the birds' faces, feet, and feathers for potential chemical repellents; and (3 inflicted mechanical damage on wasp nests to determine the defensive behavior of wasps in response to varying levels of disturbance. During caracara predation events, two species of large-bodied wasps mounted stinging attacks on caracaras, whereas three smaller-bodied wasp species did not. The "hit-and-run" predation tactic of caracaras when they attacked nests of large and aggressive wasps reduced the risk of getting stung. Our data reveal that the predation strategy of caracaras is based on mechanical disturbance of, and damage to, target wasp nests. Caracara attacks and severe experimental disturbance of nests invariably caused wasps to abscond (abandon their nests. Two compounds in caracara foot extracts [sulcatone and iridodial] elicited electrophysiological responses from wasp antennae, and were also present in defensive secretions of sympatric arboreal-nesting Azteca ants. These compounds appear not to be wasp repellents but to be acquired coincidentally by caracaras when they perch on trees inhabited with Azteca ants. We conclude that caracara predation success does not depend on wasp repellents but relies on the absconding response that is typical of swarm-founding polistine wasps. Our study highlights the potential importance of vertebrate predators in the ecology and evolution of social wasps.

  5. Thermal performance measurements on ATLAS-SCT KB forward modules

    CERN Document Server

    Donegà, M; D'Onofrio, M; Ferrère, D; Hirt, C; Ikegami, Y; Kohriki, T; Kondo, T; Lindsay, S; Mangin-Brinet, M; Niinikoski, T O; Pernegger, H; Perrin, E; Taylor, G; Terada, S; Unno, Y; Wallny, R; Weber, M

    2003-01-01

    The thermal design of the KB module is presented. A Finite Elements Analysis (FEA) has been used to finalize the module design. The thermal performance of an outer irradiated KB module has been measured at different cooling conditions. The thermal runaway of the module has been measured. The FEA model has been compared with the measurements and has been used to predict the thermal performance in a realistic SCT scenario.

  6. Cytotoxic Effect of Thymus caramanicus Jalas on Human Oral Epidermoid Carcinoma KB Cells.

    Science.gov (United States)

    Fekrazad, Reza; Afzali, Mehrad; Pasban-Aliabadi, Hamzeh; Esmaeili-Mahani, Saeed; Aminizadeh, Maryam; Mostafavi, Ali

    2017-01-01

    Identifying new chemotherapeutic agents with fewer side effects is a major concern for scientists today. Thymus caramanicus Jalas (Lamiaceae family) is one of the species of Thymus that grows wild in different regions of Iran. Traditionally, leaves of this plant are used in the treatment of diabetes, arthritis and cancer. Here was investigated the cytotoxic property of Thymus caramanicus essential oil and extract in human oral epidermoid carcinoma KB cells. Cell viability was measured by MTT and neutral red assays. The cells were exposed to different concentrations of essential oil (0.05-1 µL/mL) and extract (25-150 µg/mL) for 24 h. Doxorubicin was used as anticancer control drug. The data showed that the essential oil (IC50=0.44 µL/mL) and extract (IC50=105 µg/mL) induce potent cytotoxic property. Surprisingly, cytotoxic effects of essential oil and extract of this plant on KB cancer cells were greater than those on normal gingival HGF1-PI1 cell line. In addition, Thymus caramanicus could potentiate the effect of doxorubicin in sub-effective concentrations. The results of the present study indicate that essential oils and extracts of Thymus caramanicus have potential anti-proliferative property on KB cells and can be used as pharmaceutical case study for oral cancer treatments.

  7. The 32-kb/s ADPCM coding standard

    Energy Technology Data Exchange (ETDEWEB)

    Benvenuto, N.; Bertocci, G.; Daumer, W.R.; Sparrell, D.K.

    1986-09-01

    AT and T has played a major role in the development of national and international standards for 32-kb/s adaptive differential pulse code modulation (ADPCM). This paper highlights the process leading to the standards on 32-kb/s ADPCM recognized by the American National Standards Institute (ANSI) and the International Telephone and Telegraph Consultative Committee (CCITT). The paper briefly describes the algorithm itself and it performance. It concludes with a discussion of applications of ADPCM and the AT and T products and services that utilize ADPCM.

  8. The COHERENT Methodology in FunGramKB

    Directory of Open Access Journals (Sweden)

    Carlos Periñán-Pascual

    2011-12-01

    Full Text Available Recent research has been done synergistically between FunGramKB, a lexical-conceptual knowledge base, and the Lexical Constructional Model, a linguistic meaning construction model. since concepts are claimed to play an important role in the design of the cognitive-linguistic interface, this paper discusses the methodology adopted in structuring the basic conceptual level in the FunGramKB Core Ontology. more particularly, we describe our four-phase COHERENT methodology (i.e. COnceptualization + HiErarchization + REmodelling + refinemeNT, which guided the cognitive mapping of the defining vocabulary in Longman Dictionary of Contemporary English.

  9. Fast and complete degradation of Congo red under visible light with Er3+ and Nd3+ ions doped TiO2 nanocomposites

    Directory of Open Access Journals (Sweden)

    Himanshu Narayan

    2012-01-01

    Full Text Available We report fast and complete destruction of the dye Congo red (CR under visible light in the presence of Nd3+ and Er3+ doped TiO2 nanocomposites (NCs. TiO2 NCs with general composition TiO2[R2O3]x, {x=0.1, 0.2; R≡ Nd, Er} and particle size 12-16 nm were synthesized through co-precipitation/hydrolysis (CPH. A set of similar composites was also prepared through solid state reaction (SSR route, which produced 31- 47 nm particles. After characterization, visible light photocatalytic activity of all the samples was recorded for the degradation of CR. NCs with molar concentration x=0.2 of both doping types produced close to 100% degradation in 180 min. The apparent rate constant (kobs was found to be 2.91×10–2 min–1 and 2.36×10–2 min–1, for these Nd3+ and Er3+ doped NCs, respectively. The other NCs with x=0.1, also showed significant degradation of CR, but the samples prepared through SSR performed worse. The excellent degradation obtained with the NCs may be attributed to their small particle size. Moreover, the doping of Nd3+ and Er3+ ions further supported the photocatalysis through formation of intermediate energy levels within the band gap of TiO2. These new levels not only acted as electron traps for efficient suppression of the undesired e–/h+ recombination, but also facilitated to a certain extent the absorption of visible irradiation.

  10. The simplest possible design for a KB microfocus mirror system?

    Energy Technology Data Exchange (ETDEWEB)

    Collins, S. P., E-mail: steve.collins@diamond.ac.uk; Scott, S. M.; Hawkins, D. M.; Fabrizi, F.; Moser, B.; Nisbet, G.; Sutter, J. P. [Diamond Light Source, Harwell Science & Innovation Campus, Didcot, OX11 0DE (United Kingdom); Harwin, R. C. [Diamond Light Source, Harwell Science & Innovation Campus, Didcot, OX11 0DE (United Kingdom); Department of Physics, Cavendish Laboratory, JJ Thomson Avenue, Cambridge, CB3 0HE (United Kingdom); Harwin, W. S. [School of Systems Engineering, University of Reading, Whiteknights, Reading, Berkshire, RG6 6AH (United Kingdom)

    2016-07-27

    We report a design for a Kirkpatrick-Baez (KB) microfocussing mirror system. The main components are described, with emphasis on a ‘tripod’ manipulator, where we outline the required coordinate transformation calculations. The merit of this device lies in its simplicity of design, minimal degrees of freedom, and speed and ease of setup on a beamline. Test results and an example of the mirrors in use on Diamond Beamline I16, showing a high-resolution polar domain map of KTiOPO{sub 4} with a spot size of 1.25 µm × 1.5 µm, are presented.

  11. File list: Pol.Utr.05.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.05.AllAg.KB hg19 RNA polymerase Uterus KB SRX378799,SRX378798,SRX811394,SRX...811376,SRX811378,SRX811377,SRX811375,SRX811393 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.05.AllAg.KB.bed ...

  12. File list: Oth.Utr.50.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Utr.50.AllAg.KB hg19 TFs and others Uterus KB SRX378804,SRX811391,SRX811392,SRX...378805,SRX378797,SRX378796,SRX811372,SRX811374,SRX811373,SRX811371 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Utr.50.AllAg.KB.bed ...

  13. File list: His.Utr.05.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Utr.05.AllAg.KB hg19 Histone Uterus KB SRX811379,SRX811382,SRX811381,SRX811380,...SRX378800,SRX378801,SRX811384,SRX811386,SRX811383,SRX811385,SRX378802,SRX378803 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Utr.05.AllAg.KB.bed ...

  14. File list: Oth.Utr.20.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Utr.20.AllAg.KB hg19 TFs and others Uterus KB SRX378804,SRX811391,SRX811392,SRX...378805,SRX811373,SRX378797,SRX378796,SRX811372,SRX811374,SRX811371 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Utr.20.AllAg.KB.bed ...

  15. File list: His.Utr.20.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Utr.20.AllAg.KB hg19 Histone Uterus KB SRX811382,SRX811381,SRX811379,SRX378801,...SRX378800,SRX811386,SRX811385,SRX811383,SRX811380,SRX811384,SRX378803,SRX378802 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Utr.20.AllAg.KB.bed ...

  16. File list: Pol.Utr.20.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.20.AllAg.KB hg19 RNA polymerase Uterus KB SRX378798,SRX378799,SRX811376,SRX...811378,SRX811377,SRX811394,SRX811375,SRX811393 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.20.AllAg.KB.bed ...

  17. File list: Pol.Utr.50.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.50.AllAg.KB hg19 RNA polymerase Uterus KB SRX378799,SRX811376,SRX811378,SRX...811377,SRX378798,SRX811394,SRX811375,SRX811393 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.50.AllAg.KB.bed ...

  18. File list: His.Utr.50.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Utr.50.AllAg.KB hg19 Histone Uterus KB SRX378801,SRX378800,SRX811386,SRX811385,...SRX811379,SRX811383,SRX811381,SRX811384,SRX811380,SRX378803,SRX378802,SRX811382 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Utr.50.AllAg.KB.bed ...

  19. File list: His.Utr.10.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Utr.10.AllAg.KB hg19 Histone Uterus KB SRX811379,SRX811382,SRX811381,SRX811380,...SRX378801,SRX378800,SRX811386,SRX811385,SRX811383,SRX811384,SRX378802,SRX378803 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Utr.10.AllAg.KB.bed ...

  20. File list: Oth.Utr.05.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Utr.05.AllAg.KB hg19 TFs and others Uterus KB SRX811372,SRX811391,SRX811371,SRX...811373,SRX378804,SRX811392,SRX378805,SRX811374,SRX378797,SRX378796 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Utr.05.AllAg.KB.bed ...

  1. File list: Pol.Utr.10.AllAg.KB [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Utr.10.AllAg.KB hg19 RNA polymerase Uterus KB SRX378798,SRX378799,SRX811394,SRX...811376,SRX811378,SRX811377,SRX811375,SRX811393 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Utr.10.AllAg.KB.bed ...

  2. HINT-KB: The human interactome knowledge base

    KAUST Repository

    Theofilatos, Konstantinos A.

    2012-01-01

    Proteins and their interactions are considered to play a significant role in many cellular processes. The identification of Protein-Protein interactions (PPIs) in human is an open research area. Many Databases, which contain information about experimentally and computationally detected human PPIs as well as their corresponding annotation data, have been developed. However, these databases contain many false positive interactions, are partial and only a few of them incorporate data from various sources. To overcome these limitations, we have developed HINT-KB (http://150.140.142.24:84/Default.aspx) which is a knowledge base that integrates data from various sources, provides a user-friendly interface for their retrieval, estimates a set of features of interest and computes a confidence score for every candidate protein interaction using a modern computational hybrid methodology. © 2012 IFIP International Federation for Information Processing.

  3. SAFOD Brittle Microstructure and Mechanics Knowledge Base (BM2KB)

    Science.gov (United States)

    Babaie, Hassan A.; Broda Cindi, M.; Hadizadeh, Jafar; Kumar, Anuj

    2013-07-01

    Scientific drilling near Parkfield, California has established the San Andreas Fault Observatory at Depth (SAFOD), which provides the solid earth community with short range geophysical and fault zone material data. The BM2KB ontology was developed in order to formalize the knowledge about brittle microstructures in the fault rocks sampled from the SAFOD cores. A knowledge base, instantiated from this domain ontology, stores and presents the observed microstructural and analytical data with respect to implications for brittle deformation and mechanics of faulting. These data can be searched on the knowledge base‧s Web interface by selecting a set of terms (classes, properties) from different drop-down lists that are dynamically populated from the ontology. In addition to this general search, a query can also be conducted to view data contributed by a specific investigator. A search by sample is done using the EarthScope SAFOD Core Viewer that allows a user to locate samples on high resolution images of core sections belonging to different runs and holes. The class hierarchy of the BM2KB ontology was initially designed using the Unified Modeling Language (UML), which was used as a visual guide to develop the ontology in OWL applying the Protégé ontology editor. Various Semantic Web technologies such as the RDF, RDFS, and OWL ontology languages, SPARQL query language, and Pellet reasoning engine, were used to develop the ontology. An interactive Web application interface was developed through Jena, a java based framework, with AJAX technology, jsp pages, and java servlets, and deployed via an Apache tomcat server. The interface allows the registered user to submit data related to their research on a sample of the SAFOD core. The submitted data, after initial review by the knowledge base administrator, are added to the extensible knowledge base and become available in subsequent queries to all types of users. The interface facilitates inference capabilities in the

  4. Graphics Intro 64kB Using OpenGL

    OpenAIRE

    Obrtlík, Petr

    2016-01-01

    Tato bakalářská práce je zaměřena na problematiku tvorby grafického intra s omezenou velikostí. Práce popisuje využívané metody pro tvorbu grafického intra. Zabývá se procedurálním generováním objektů a textur, jednoduchým generováním částic, vykreslováním vodní hladiny s reflexí a refrakcí, vytvářením stínů, osvětlení, nastavením kompilátoru a komprimací výsledného souboru. Výsledkem je kreslená krajinka simulující průběh dne. Vytvořené grafické intro má velikost menší než 64kB. This bach...

  5. Simple and fast method for iron determination in white and red wines using dispersive liquid-liquid microextraction and ultraviolet-visible spectrophotometry.

    Science.gov (United States)

    Maciel, Juliana V; Soares, Bruno M; Mandlate, Jaime S; Picoloto, Rochele S; Bizzi, Cezar A; Flores, Erico M M; Duarte, Fabio A

    2014-08-20

    This work reports the development of a method for Fe extraction in white and red wines using dispersive liquid-liquid microextraction (DLLME) and determination by ultraviolet-visible spectrophotometry. For optimization of the DLLME method, the following parameters were evaluated: type and volume of dispersive (1300 μL of acetonitrile) and extraction (80 μL of C(2)Cl(4)) solvents, pH (3.0), concentration of ammonium pyrrolidinedithiocarbamate (APDC, 500 μL of 1% m/v APDC solution), NaCl concentration (not added), and extraction time. The calibration curve was performed using the analyte addition method, and the limit of detection and relative standard deviation were 0.2 mg L(-1) and below 7%, respectively. The accuracy was evaluated by comparison of results obtained after Fe determination by graphite furnace atomic absorption spectrometry, with agreement ranging from 94 to 105%. The proposed method was applied for Fe determination in white and red wines with concentrations ranging from 1.3 to 4.7 mg L(-1).

  6. Stable isotope models of sugar intake using hair, red blood cells, and plasma, but not fasting plasma glucose, predict sugar intake in a Yup'ik study population.

    Science.gov (United States)

    Nash, Sarah H; Kristal, Alan R; Hopkins, Scarlett E; Boyer, Bert B; O'Brien, Diane M

    2014-01-01

    Objectively measured biomarkers will help to resolve the controversial role of sugar intake in the etiology of obesity and related chronic diseases. We recently validated a dual-isotope model based on RBC carbon (δ(13)C) and nitrogen (δ(15)N) isotope ratios that explained a large percentage of the variation in self-reported sugar intake in a Yup'ik study population. Stable isotope ratios can easily be measured from many tissues, including RBCs, plasma, and hair; however, it is not known how isotopic models of sugar intake compare among these tissues. Here, we compared self-reported sugar intake with models based on RBCs, plasma, and hair δ(13)C and δ(15)N in Yup'ik people. We also evaluated associations of sugar intake with fasting plasma glucose δ(13)C. Finally, we evaluated relations between δ(13)C and δ(15)N values in hair, plasma, RBCs, and fasting plasma glucose to allow comparison of isotope ratios across tissue types. Models using RBCs, plasma, or hair isotope ratios explained similar amounts of variance in total sugar, added sugar, and sugar-sweetened beverage intake (∼53%, 48%, and 34%, respectively); however, the association with δ(13)C was strongest for models based on RBCs and hair. There were no associations with fasting plasma glucose δ(13)C (R(2) = 0.03). The δ(13)C and δ(15)N values of RBCs, plasma, and hair showed strong, positive correlations; the slopes of these relations did not differ from 1. This study demonstrates that RBC, plasma, and hair isotope ratios predict sugar intake and provides data that will allow comparison of studies using different sample types.

  7. Fast and efficient: postnatal growth and energy expenditure in an Arctic-breeding waterbird, the Red-throated Loon (Gavia stellata)

    Science.gov (United States)

    Rizzolo, Daniel; Schmutz, Joel A.; Speakman, John R.

    2015-01-01

    Environmental conditions can exert a strong influence on the growth and energy demands of chicks. We hypothesized that postnatal growth in a cold, aquatic environment would require a high level of energy metabolism in semiprecocial Red-throated Loon (Gavia stellata) chicks. We measured body-mass growth and daily energy expenditure (DEE) of free-ranging chicks in the Arctic. We used daily gains in body mass and DEE to estimate daily metabolizable energy (DME, kJ day-1) and total metabolizable energy (TME, kJ chick-1). Chicks gained body mass quickly, with a logistic growth rate constant 57% greater than the allometric prediction, yet were at only 60% of adult body mass at fledging. Males grew at a rate similar to that of females but for a slightly longer duration and so reached an asymptotic body mass 23% greater, and tarsus length 8% longer, than that of females. Chick growth performance was similar between first- and second-hatched chicks within broods of 2, which suggests that food availability was not limited. DEE increased in proportion to body mass, and DME peaked at 1,214 kJ day-1 on day 25 posthatching. Over the average 49-day postnatal period, TME was 49.0 MJ, which is within the range of error of the allometric prediction. Parents provided 58.6 MJ as food to meet this energy requirement. Given this chick energy requirement and the range of energy content of prey observed in the chick diet, selecting prey with higher energy content would greatly reduce adult provisioning effort. Red-throated Loon chicks did not have a high postnatal energy requirement, but rather grew quickly and fledged at a small size-with the effect of reducing the length of the postnatal period and, consequently, parental energy investment in chicks.

  8. An Infra-Red Heating Based Fast Method of Moisture Content Measurement and Its Application to Measure Blend Proportion of Polyester-Viscose Woven Fabrics

    Directory of Open Access Journals (Sweden)

    V.K. Kothari

    2010-06-01

    Full Text Available Moisture content values of plain woven fabrics of polyester-viscose blended yarns were measured using standard conditioning procedures as well as a new method of preconditioning of samples using infra-red heating and subsequent conditioning in a desiccator for a specific conditioning time. The effects of drying time and conditioning time on measured moisture content values were studied and optimization of these parameters were done. Results of the moisture content measurement using these methods show a sufficiently linear relationship between measured moisture content and the proportion of viscose in the fabric samples. The regression analysis showed very high coefficients of determination (above 0.98 in all cases. Regression equations were used to predict the blend proportion of fabric samples from the moisture content data. It was found that even the moisture content values using a shorter conditioning time of 60 minutes and even 10 minutes yielded correct prediction with a maximum error of magnitude less than 3.0 in blend percentage values.

  9. Developmental regulation of a complete 70kb human β-globin locus in transgenic mice.

    NARCIS (Netherlands)

    J. Strouboulis (John); N.O. Dillon (Niall); F.G. Grosveld (Frank)

    1992-01-01

    textabstractWe have used a linker-based ligation strategy to combine two 35-kb cosmid inserts from the human beta-globin locus into one linear fragment containing the entire locus. This 70-kb fragment was introduced into transgenic mice by microinjection of fertilized eggs. Southern blot analysis sh

  10. Collateral sensitivity to cisplatin in KB-8-5-11 drug-resistant cancer cells.

    LENUS (Irish Health Repository)

    Doherty, Ben

    2014-01-01

    KB-8-5-11 cells are a drug-resistant cervical cell model that overexpresses ABCB1 (P-glycoprotein). KB-8-5-11 has become sensitive to non-ABCB1 substrate cisplatin. Understanding the mechanism of collateral sensitivity to cisplatin may lead to biomarker discovery for platinum sensitivity in patients with cancer.

  11. Instruments for competing claims on natural resources : preliminary assessment of KB 2011 projects

    NARCIS (Netherlands)

    Silvis, H.J.; Bodegom, van A.J.

    2011-01-01

    This report results from the project ‘Competing Claims on Natural Resources (overkoepelend instrumentarium)’ in the KB programme of 2011. In short the terms of reference of this report are as follows: Give an overview of instruments developed in other KB projects relevant for the analysis of Competi

  12. Radiative transfer in disc galaxies $-$ V. The accuracy of the KB approximation

    CERN Document Server

    Lee, Dukhang; Seon, Kwang-Il; Camps, Peter; Verstocken, Sam; Han, Wonyong

    2016-01-01

    We investigate the accuracy of an approximate radiative transfer technique that was first proposed by Kylafis & Bahcall (hereafter the KB approximation) and has been popular in modelling dusty late-type galaxies. We compare realistic galaxy models calculated with the KB approximation with those of a three-dimensional Monte Carlo radiative transfer code SKIRT. The SKIRT code fully takes into account of the contribution of multiple scattering whereas the KB approximation calculates only single scattered intensity and multiple scattering components are approximated. We find that the KB approximation gives fairly accurate results if optically thin, face-on galaxies are considered. However, for highly inclined ($i \\gtrsim 85^{\\circ}$) and/or optically thick (central face-on optical depth $\\gtrsim1$) galaxy models, the approximation can give rise to substantial errors, sometimes, up to $\\gtrsim 40\\%$. Moreover, it is also found that the KB approximation is not always physical, sometimes producing infinite inten...

  13. Radiative transfer in disc galaxies - V. The accuracy of the KB approximation

    Science.gov (United States)

    Lee, Dukhang; Baes, Maarten; Seon, Kwang-Il; Camps, Peter; Verstocken, Sam; Han, Wonyong

    2016-12-01

    We investigate the accuracy of an approximate radiative transfer technique that was first proposed by Kylafis & Bahcall (hereafter the KB approximation) and has been popular in modelling dusty late-type galaxies. We compare realistic galaxy models calculated with the KB approximation with those of a three-dimensional Monte Carlo radiative transfer code SKIRT. The SKIRT code fully takes into account of the contribution of multiple scattering whereas the KB approximation calculates only single scattered intensity and multiple scattering components are approximated. We find that the KB approximation gives fairly accurate results if optically thin, face-on galaxies are considered. However, for highly inclined (i ≳ 85°) and/or optically thick (central face-on optical depth ≳1) galaxy models, the approximation can give rise to substantial errors, sometimes, up to ≳40 per cent. Moreover, it is also found that the KB approximation is not always physical, sometimes producing infinite intensities at lines of sight with high optical depth in edge-on galaxy models. There is no `simple recipe' to correct the errors of the KB approximation that is universally applicable to any galaxy models. Therefore, it is recommended that the full radiative transfer calculation be used, even though it is slower than the KB approximation.

  14. Eye redness

    Science.gov (United States)

    Bloodshot eyes; Red eyes; Scleral injection; Conjunctival injection ... There are many causes of a red eye or eyes. Some are medical emergencies. Others are a cause for concern, but not an emergency. Many are nothing to worry about. Eye ...

  15. Targeted images of KB cells using folate-conjugated gold nanoparticles

    Science.gov (United States)

    Rathinaraj, Pierson; Lee, Kyubae; Park, Soo-Young; Kang, Inn-Kyu

    2015-01-01

    Mercaptosuccinic acid-coated gold (GM) nanoparticles were prepared and characterized by transmission electron microscopy and dynamic light scattering. Folic acid (F) was then conjugated to the GM to preferentially target oral squamous cancer (KB) cells with folate receptors expressed on their membranes and facilitate the transit of the nanoparticles across the cell membrane. Finally, a fluorescence dye (Atto) was conjugated to the nanoparticles to visualize their internalization into KB cells. After culture of the cells in a medium containing GM and folate-conjugated GM (GF), the interaction of surface-modified gold nanoparticles with KB cells was studied.

  16. A Role for the NF-kb/Rel Transcription Factors in Human Breast Cancer

    Science.gov (United States)

    1998-07-01

    cell system. We initiated our studies using the human fibrosar- coma cell line HT1080 , which is relatively resistant to killing by TNF (4... fibrosarcoma cell line . This cell line was chosen since it is highly resistant to killing by TNF. Expression of IKBOC blocks NF-KB nuclear translocation...To potentially block the activation of NF-KB in response to TNF stimulation, we established an HT1080 cell line (HT1080I) expressing a

  17. Instruments for competing claims on natural resources : preliminary assessment of KB 2011 projects

    OpenAIRE

    2011-01-01

    This report results from the project ‘Competing Claims on Natural Resources (overkoepelend instrumentarium)’ in the KB programme of 2011. In short the terms of reference of this report are as follows: Give an overview of instruments developed in other KB projects relevant for the analysis of Competing Claims on Natural Resources. Questions include: What are feasible instruments? What are their advantages and disadvantages? What can or should be applied in specific cases? Is there a need to va...

  18. Paradoxical effects of KB-R7943 on arrhythmogenicity in a chronic myocardial infarction rabbit model.

    Science.gov (United States)

    Chang, Po-Cheng; Wo, Hung-Ta; Lee, Hui-Ling; Wen, Ming-Shien; Chou, Chung-Chuan

    2015-07-01

    Na(+)/Ca(2+) exchanger blockade has been reported to be anti-arrhythmic in different models. The effects of KB-R7943, a Na(+)/Ca(2+) exchanger blocker, on arrhythmogenesis in hearts with chronic myocardial infarction (MI) remain unclear. Dual voltage and intracellular Ca(2+) (Cai) optical mapping was performed in nine rabbit hearts with chronic MI and four control hearts. Electrophysiology studies including inducibility of ventricular tachyarrhythmias, ventricular fibrillation dominant frequency, action potential, Cai alternans, Cai decay, and conduction velocity were performed. The same protocol was repeated in the presence of KB-R7943 (0.5, 1, and 5μM) after the baseline studies. KB-R7943 was effective in suppressing afterdepolarizations and spontaneous ventricular tachyarrhythmias in hearts with chronic MI. Surprisingly, KB-R7943 increased the inducibility of ventricular tachyarrhythmias in a dose-dependent manner (11%, 11%, 22%, and 56% at baseline and with 0.5, 1, and 5μM KB-R7943, respectively, p=0.02). Optical mapping analysis revealed that the underlying mechanisms of the induced ventricular tachyarrhythmias were probably spatially discordant alternans with wave breaks and rotors. Further analysis showed that KB-R7943 significantly enhanced both action potential (p=0.033) and Cai (p=0.001) alternans, prolonged Cai decay (tau value) in a dose-dependent manner (p=0.004), and caused heterogeneous conduction delay especially at peri-infarct zones during rapid burst pacing. In contrast, KB-R7943 had insignificant effects in control hearts. In this chronic MI rabbit model, KB-R7943 has contrasting effects on arrhythmogenesis, suppressing afterdepolarizations and spontaneous ventricular tachyarrhythmias, but enhancing the inducibility of tachyarrhythmias. The mechanism is probably the enhanced spatially discordant alternans because of prolonged Cai decay and heterogeneous conduction delay. Copyright © 2014 Japanese College of Cardiology. Published by Elsevier

  19. Quercetin induces apoptosis via the mitochondrial pathway in KB and KBv200 cells.

    Science.gov (United States)

    Zhang, Jian-ye; Yi, Tao; Liu, Jing; Zhao, Zhong-zhen; Chen, Hu-biao

    2013-03-06

    In this study, anticancer activities of six compounds of flavonoids were investigated in human epidermoid carcinoma KB and KBv200 cells. Among these compounds, quercetin and acacetin showed strong inhibition of cell growth in KB and KBv200 cells. IC50 values of quercetin against KB and KBv200 cells were 17.84 ± 4.14 and 18.94 ± 4.75 μM, respectively. The IC50 values of acacetin against KB and KBv200 cells were 41.33 ± 6.05 and 49.04 ± 3.64 μM. The IC50 values of apigenin, kaempferol, kaempferol 3-O-rhamnoside, and quercetin 3-O-rhamnoside were more than 100 μM. Furthermore, quercetin was found to induce apoptosis in KB and KBv200 cells via the mitochondrial pathway, including a decrease of the reactive oxygen species level, loss of mitochondrial membrane potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly (ADP-ribose) polymerase. The apoptosis induced by quercetin was not related to the regulation of Bcl-2 or Bax in KB and KBv200 cells.

  20. FragKB: structural and literature annotation resource of conserved peptide fragments and residues.

    Directory of Open Access Journals (Sweden)

    Ashish V Tendulkar

    Full Text Available BACKGROUND: FragKB (Fragment Knowledgebase is a repository of clusters of structurally similar fragments from proteins. Fragments are annotated with information at the level of sequence, structure and function, integrating biological descriptions derived from multiple existing resources and text mining. METHODOLOGY: FragKB contains approximately 400,000 conserved fragments from 4,800 representative proteins from PDB. Literature annotations are extracted from more than 1,700 articles and are available for over 12,000 fragments. The underlying systematic annotation workflow of FragKB ensures efficient update and maintenance of this database. The information in FragKB can be accessed through a web interface that facilitates sequence and structural visualization of fragments together with known literature information on the consequences of specific residue mutations and functional annotations of proteins and fragment clusters. FragKB is accessible online at http://ubio.bioinfo.cnio.es/biotools/fragkb/. SIGNIFICANCE: The information presented in FragKB can be used for modeling protein structures, for designing novel proteins and for functional characterization of related fragments. The current release is focused on functional characterization of proteins through inspection of conservation of the fragments.

  1. Aging-kb: a knowledge base for the study of the Aging process

    Science.gov (United States)

    Becker, Kevin G.; Holmes, Karen A.; Zhang, Yongqing

    2012-01-01

    As the science of the aging process moves forward, a recurring challenge is the integration of multiple types of data and information with classical aging theory while disseminating that information to the scientific community. Here we present AGING-kb, a public knowledge base with the goal of conceptualizing and presenting fundamental aspects of the study of the aging process. Aging-kb has two interconnected parts, the Aging-kb tree and the Aging Wiki. The Aging-kb tree is a simple intuitive dynamic tree hierarchy of terms describing the field of aging from the general to the specific. This enables the user to see relationships between areas of aging research in a logical comparative fashion. The second part is a specialized Aging Wiki which allows expert definition, description, supporting information, and documentation of each aging keyword term found in the Aging-kb tree. The Aging Wiki allows community participation in describing and defining concepts and terms in the Wiki format. This aging knowledge base provides a simple intuitive interface to the complexities of aging. http://aging-kb.nia.nih.gov PMID:22100666

  2. Resolving arthropod phylogeny: exploring phylogenetic signal within 41 kb of protein-coding nuclear gene sequence.

    Science.gov (United States)

    Regier, Jerome C; Shultz, Jeffrey W; Ganley, Austen R D; Hussey, April; Shi, Diane; Ball, Bernard; Zwick, Andreas; Stajich, Jason E; Cummings, Michael P; Martin, Joel W; Cunningham, Clifford W

    2008-12-01

    This study attempts to resolve relationships among and within the four basal arthropod lineages (Pancrustacea, Myriapoda, Euchelicerata, Pycnogonida) and to assess the widespread expectation that remaining phylogenetic problems will yield to increasing amounts of sequence data. Sixty-eight regions of 62 protein-coding nuclear genes (approximately 41 kilobases (kb)/taxon) were sequenced for 12 taxonomically diverse arthropod taxa and a tardigrade outgroup. Parsimony, likelihood, and Bayesian analyses of total nucleotide data generally strongly supported the monophyly of each of the basal lineages represented by more than one species. Other relationships within the Arthropoda were also supported, with support levels depending on method of analysis and inclusion/exclusion of synonymous changes. Removing third codon positions, where the assumption of base compositional homogeneity was rejected, altered the results. Removing the final class of synonymous mutations--first codon positions encoding leucine and arginine, which were also compositionally heterogeneous--yielded a data set that was consistent with a hypothesis of base compositional homogeneity. Furthermore, under such a data-exclusion regime, all 68 gene regions individually were consistent with base compositional homogeneity. Restricting likelihood analyses to nonsynonymous change recovered trees with strong support for the basal lineages but not for other groups that were variably supported with more inclusive data sets. In a further effort to increase phylogenetic signal, three types of data exploration were undertaken. (1) Individual genes were ranked by their average rate of nonsynonymous change, and three rate categories were assigned--fast, intermediate, and slow. Then, bootstrap analysis of each gene was performed separately to see which taxonomic groups received strong support. Five taxonomic groups were strongly supported independently by two or more genes, and these genes mostly belonged to the slow

  3. Experimental information - RED | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ba.nbdc00315-003 Description of data contents Detailed information of the experiment of each array glass. Da...ta file File name: red_experiment_info.zip File URL: ftp://ftp.biosciencedbc.jp/archive/red/LATEST/red_experiment... 2,158 entries Data item Description Info ID ID of the experimental information Research ID Research ID (Sub...rsion) g : Rice8987 g_array (Full insert version) others : Other types blank : Unknown glass ID Array glass ID experiment..._info.zip File size: 20.3 KB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/red_experim

  4. Rapid and accurate detection of the CFTR gene mutation 1811+1.6 kbA>G by real-time fluorescence resonance energy transfer PCR.

    Science.gov (United States)

    Reboul, Marie-Pierre; Higueret, Laurent; Biteau, Nicolas; Iron, Albert

    2005-10-01

    The CFTR gene mutation 1811+1.6 kbA>G has been reported as associated with a severe phenotype of cystic fibrosis with pancreatic insufficiency. This mutation has been identified as a rather common one in the South West of France and in the Iberian Peninsula. Because of the precise geographical origin of the subjects and its frequency, the mutation has to be investigated with accuracy. We have developed an original real-time Fluorescence Resonance Energy Transfer (FRET) PCR assay for genotyping the mutation 1811+1.6 kbA>G. It is based on the amplification of a region spanning the mutation with simultaneous detection of the amplicon by hybridization with a bi-probe followed by a melting curve analysis. The results obtained are identical with those resulting from either restriction fragment length polymorphism analysis or sequencing. The distinction between the wild type and the mutation 1811+1.6 kbA>G is easy because the corresponding melting points shows a difference of 6 or 9.5 degrees C depending on the associated SNP A/T located 16 bp downstream. We demonstrated that a FRET assay showed enough sensitivity to discriminate between two nucleotide polymorphisms (SNPs) in the sequence of the sensor. In conclusion, this method is specific, fast, easy to perform, reproducible, inexpensive as it uses only one bi-probe and well adapted to daily practice.

  5. Transcriptional organization of a 450-kb region of the human X chromosome in Xq28

    Energy Technology Data Exchange (ETDEWEB)

    Bione, S.; Tamanini, F.; Maestrini, E.; Tribioli, C.; Rivella, S.; Toniolo, D. (Instituto di Genetica Biochimica ed Evoluzionistica, Pavia (Italy)); Poustka, A. (German Cancer Research Center, Heidelberg (Germany))

    1993-11-15

    In this paper, the authors report the transcriptional organization of a 450-kb gene cluster in Xq28, flanked by the glucose-6-phosphate dehydrogenase and the color vision genes. CpG islands previously identified and mapped to distal Xq28 have helped in construction of a continuous contig of cosmids and in identification of cDNAs corresponding to eight transcripts. Thirteen to 16 small genes with CpG islands are clustered in a region of 250-300 kb. Many are highly expressed in muscle or brain and may be the genes responsible for muscle or neurological disorders mapped to distal Xq28. The analysis indicates that, in this region of the genome, genes not related in sequence are organized in transcriptional domains of 100 kb and that this organization may be important for establishing and regulating gene expression in relation to tissue distribution and X chromosome inactivation.

  6. Transcriptional organization of a 450-kb region of the human X chromosome in Xq28.

    Science.gov (United States)

    Bione, S; Tamanini, F; Maestrini, E; Tribioli, C; Poustka, A; Torri, G; Rivella, S; Toniolo, D

    1993-12-01

    In this paper, we report the transcriptional organization of a 450-kb gene cluster in Xq28, flanked by the glucose-6-phosphate dehydrogenase and the color vision genes. CpG islands previously identified and mapped to distal Xq28 have helped in construction of a continuous contig of cosmids and in identification of cDNAs corresponding to eight transcripts. Thirteen to 16 small genes with CpG islands are clustered in a region of 250-300 kb. Many are highly expressed in muscle or brain and may be the genes responsible for muscle or neurological disorders mapped to distal Xq28. Our analysis indicates that, in this region of the genome, genes not related in sequence are organized in transcriptional domains of 100 kb and that this organization may be important for establishing and regulating gene expression in relation to tissue distribution and X chromosome inactivation.

  7. A low-delay 8 Kb/s backward-adaptive CELP coder

    Science.gov (United States)

    Neumeyer, L. G.; Leblanc, W. P.; Mahmoud, S. A.

    1990-01-01

    Code excited linear prediction coding is an efficient technique for compressing speech sequences. Communications quality of speech can be obtained at bit rates below 8 Kb/s. However, relatively large coding delays are necessary to buffer the input speech in order to perform the LPC analysis. A low delay 8 Kb/s CELP coder is introduced in which the short term predictor is based on past synthesized speech. A new distortion measure that improves the tracking of the formant filter is discussed. Formal listening tests showed that the performance of the backward adaptive coder is almost as good as the conventional CELP coder.

  8. Pressure derivatives of elastic moduli of fused quartz to 10 kb

    Science.gov (United States)

    Peselnick, L.; Meister, R.; Wilson, W.H.

    1967-01-01

    Measurements of the longitudinal and shear moduli were made on fused quartz to 10 kb at 24??5??C. The anomalous behavior of the bulk modulus K at low pressure, ???K ???P 0, at higher pressures. The pressure derivative of the rigidity modulus ???G ???P remains constant and negative for the pressure range covered. A 15-kb hydrostatic pressure vessel is described for use with ultrasonic pulse instrumentation for precise measurements of elastic moduli and density changes with pressure. The placing of the transducer outside the pressure medium, and the use of C-ring pressure seals result in ease of operation and simplicity of design. ?? 1967.

  9. Birthmarks - red

    Science.gov (United States)

    Strawberry mark; Vascular skin changes; Angioma cavernosum; Capillary hemangioma; Hemangioma simplex ... There are 2 main categories of birthmarks: Red birthmarks are made ... are called vascular birthmarks. Pigmented birthmarks are areas ...

  10. Data of evolutionary structure change: 1I7KB-2ONUA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1I7KB-2ONUA 1I7K 2ONU B A PVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHG.../entryChain> 2ONU A 2ONUA LEU CA 387 2ONU A 2ONU...248 THR CA 202 ALA CA 208 PHE CA 291 2ONU... A 2ONUA

  11. Band structure and optical properties of LiKB4O7 single crystal

    NARCIS (Netherlands)

    Smok, P; Seinert, H; Kityk, [No Value; Berdowski, J

    2003-01-01

    The band structure (BS), electronic charge density distribution and linear optical properties of the LiKB4O7 (LKB4) single crystal are calculated using a self-consistent norm-conserving pseudo-potential method within the framework of the local density approximation theory. Dispersion of the imaginar

  12. Band structure and optical properties of LiKB4O7 single crystal

    NARCIS (Netherlands)

    Smok, P; Seinert, H; Kityk, [No Value; Berdowski, J

    2003-01-01

    The band structure (BS), electronic charge density distribution and linear optical properties of the LiKB4O7 (LKB4) single crystal are calculated using a self-consistent norm-conserving pseudo-potential method within the framework of the local density approximation theory. Dispersion of the imaginar

  13. Tanshinone IIA Induces Apoptosis in Human Oral Cancer KB Cells through a Mitochondria-Dependent Pathway

    Directory of Open Access Journals (Sweden)

    Pao-Yu Tseng

    2014-01-01

    Full Text Available Tanshinone IIA (Tan IIA, an active phytochemical in the dried root of Salvia miltiorrhiza Bunge, has shown an antiproliferative activity on various human cancer cell lines including nasopharyngeal carcinoma cells. However, the effects of Tan IIA on human oral cancer cells are still unknown. This study aimed to investigate the antiproliferative effects of Tan IIA on human oral cancer KB cells and explored the possible underlying mechanism. Treatment of KB cells with Tan IIA suppressed cell proliferation/viability and induced cell death in a dose-dependent manner through sulforhodamine B colorimetric assay. Observation of cell morphology revealed the involvement of apoptosis in the Tan IIA-induced growth inhibition on KB cells. Cell cycle analysis showed a cell cycle arrest in G2/M phase on Tan IIA-treated cells. The dissipation of mitochondrial membrane potential observed by flow cytometry and the expression of activated caspases with the cleaved poly (ADP-ribose polymerase under immunoblotting analysis indicated that Tan IIA-induced apoptosis in KB cells was mediated through the mitochondria-dependent caspase pathway. These observations suggested that Tan IIA could be a potential anticancer agent for oral cancer.

  14. Introducing the Forensic Research/Reference on Genetics knowledge base, FROG-kb

    Directory of Open Access Journals (Sweden)

    Rajeevan Haseena

    2012-09-01

    Full Text Available Abstract Background Online tools and databases based on multi-allelic short tandem repeat polymorphisms (STRPs are actively used in forensic teaching, research, and investigations. The Fst value of each CODIS marker tends to be low across the populations of the world and most populations typically have all the common STRP alleles present diminishing the ability of these systems to discriminate ethnicity. Recently, considerable research is being conducted on single nucleotide polymorphisms (SNPs to be considered for human identification and description. However, online tools and databases that can be used for forensic research and investigation are limited. Methods The back end DBMS (Database Management System for FROG-kb is Oracle version 10. The front end is implemented with specific code using technologies such as Java, Java Servlet, JSP, JQuery, and GoogleCharts. Results We present an open access web application, FROG-kb (Forensic Research/Reference on Genetics-knowledge base, http://frog.med.yale.edu, that is useful for teaching and research relevant to forensics and can serve as a tool facilitating forensic practice. The underlying data for FROG-kb are provided by the already extensively used and referenced ALlele FREquency Database, ALFRED (http://alfred.med.yale.edu. In addition to displaying data in an organized manner, computational tools that use the underlying allele frequencies with user-provided data are implemented in FROG-kb. These tools are organized by the different published SNP/marker panels available. This web tool currently has implemented general functions possible for two types of SNP panels, individual identification and ancestry inference, and a prediction function specific to a phenotype informative panel for eye color. Conclusion The current online version of FROG-kb already provides new and useful functionality. We expect FROG-kb to grow and expand in capabilities and welcome input from the forensic community in

  15. Introducing the Forensic Research/Reference on Genetics knowledge base, FROG-kb

    Science.gov (United States)

    2012-01-01

    Background Online tools and databases based on multi-allelic short tandem repeat polymorphisms (STRPs) are actively used in forensic teaching, research, and investigations. The Fst value of each CODIS marker tends to be low across the populations of the world and most populations typically have all the common STRP alleles present diminishing the ability of these systems to discriminate ethnicity. Recently, considerable research is being conducted on single nucleotide polymorphisms (SNPs) to be considered for human identification and description. However, online tools and databases that can be used for forensic research and investigation are limited. Methods The back end DBMS (Database Management System) for FROG-kb is Oracle version 10. The front end is implemented with specific code using technologies such as Java, Java Servlet, JSP, JQuery, and GoogleCharts. Results We present an open access web application, FROG-kb (Forensic Research/Reference on Genetics-knowledge base, http://frog.med.yale.edu), that is useful for teaching and research relevant to forensics and can serve as a tool facilitating forensic practice. The underlying data for FROG-kb are provided by the already extensively used and referenced ALlele FREquency Database, ALFRED (http://alfred.med.yale.edu). In addition to displaying data in an organized manner, computational tools that use the underlying allele frequencies with user-provided data are implemented in FROG-kb. These tools are organized by the different published SNP/marker panels available. This web tool currently has implemented general functions possible for two types of SNP panels, individual identification and ancestry inference, and a prediction function specific to a phenotype informative panel for eye color. Conclusion The current online version of FROG-kb already provides new and useful functionality. We expect FROG-kb to grow and expand in capabilities and welcome input from the forensic community in identifying datasets and

  16. Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157:H7 derived from Sakai outbreak.

    Science.gov (United States)

    Makino, K; Ishii, K; Yasunaga, T; Hattori, M; Yokoyama, K; Yutsudo, C H; Kubota, Y; Yamaichi, Y; Iida, T; Yamamoto, K; Honda, T; Han, C G; Ohtsubo, E; Kasamatsu, M; Hayashi, T; Kuhara, S; Shinagawa, H

    1998-02-28

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7, derived from an outbreak in Sakai city, Japan in 1996, possesses two kinds of plasmids: a 93-kb plasmid termed pO157, found in clinical EHEC isolates world-wide and a 3.3-kb plasmid termed pOSAK1, prevalent in EHEC strains isolated in Japan. Complete nucleotide sequences of both plasmids have been determined, and the putative functions of the encoded proteins and the cis-acting DNA sequences have been analyzed. pO157 shares strikingly similar genes and DNA sequences with F-factor and the transmissible drug-resistant plasmid R100 for DNA replication, copy number control, plasmid segregation, conjugative functions and stable maintenance in the host, although it is defective in DNA transfer by conjugation due to the truncation and deletion of the required genes and DNA sequences. In addition, it encodes several proteins implicated in EHEC pathogenicity such as an EHEC hemolysin (HlyA), a catalase-peroxidase (KatP), a serine protease (EspP) and type II secretion system. pOSAK1 possesses a ColE1-like replication system, and the DNA sequence is extremely similar to that of a drug-resistant plasmid, NTP16, derived from Salmonella typhimurium except that it lacks drug resistance transposons.

  17. Product (RED)

    DEFF Research Database (Denmark)

    Ponte, Stefano

    2011-01-01

    for a better world) by enrolling consumers in ways that do not rely on accurate knowledge of the products or specific understanding of the cause that The Global Fund engages but, instead, rely on a system of more general, affective affinity between the ‘aid celebrities’ who are behind RED (such as Bono......) and the consumers who buy iconic brand products to help ‘distant others’. While in many other forms of causumerism, labels or certification systems ‘prove’ that a product is just, in RED, aid celebrities provide the proof. From the consumer point of view both labels and celebrities provide a similar simplification...... a proportion of sales income to help distant others in Africa. The iconic brands sitting under the RED umbrella also play an important role as they offer a consistent and known venue for channeling consumer affect. We argue that celebrity validation, backed up by iconic brands, facilitates at least three...

  18. Identification of Hox genes and rearrangements within the single homeobox (Hox) cluster (192.8 kb) of the cyclopoid copepod (Paracyclopina nana).

    Science.gov (United States)

    Kim, Hui-Su; Kim, Bo-Mi; Lee, Bo-Young; Souissi, Sami; Park, Heum Gi; Lee, Jae-Seong

    2016-03-01

    We report the first identification of the entire complement of the eight typical homeobox (hox) genes (lab, pb, Dfd, scr, antp, ubx, Abd-A, and Abd-B) and the ftz gene in a 192.8 kb region in the cyclopoid copepod Paracyclopina nana. A Hox3 gene ortholog was not present in the P. nana hox gene cluster, while the P. nana Dfd gene was transcribed in the opposite direction to the Daphnia pulex Dfd gene, but in the same direction as the Dfd genes of the fruit fly Drosophila melanogaster and red flour beetle Tribolium castaneum. The location of the lab and pb genes was switched in the P. nana hox cluster, while the order of the remaining hox genes was generally conserved with those of other arthropods. J. Exp. Zool. (Mol. Dev. Evol.) 9999B:XX-XX, 2016. © 2016 Wiley Periodicals, Inc.

  19. Cytotoxicity against KB and NCI-H187 cell lines of modified flavonoids from Kaempferia parviflora.

    Science.gov (United States)

    Yenjai, Chavi; Wanich, Suchana

    2010-05-01

    Flavones 1-4 isolated from Kaempferia parviflora were used for structural modification. Sixteen flavonoid derivatives, including four new derivatives, were synthesized and evaluated for cytotoxicity against KB and NCI-H187 cell lines. Flavanones 2a-4a demonstrated higher cytotoxic activity than the parent compounds. Cytotoxicity against KB cell line of oxime 1c was about 7 times higher than the ellipticine standard. Interestingly, oximes 1c and 2c exhibited highly potent cytotoxicity against NCI-H187 cell line with IC(50) values of 0.014 and 0.23 microM, respectively. Oximes 4c and 5c showed strong cytotoxicity against NCI-H187 cell line with IC(50) values of 4.04 and 2.32 microM, respectively. 2010 Elsevier Ltd. All rights reserved.

  20. The apoptosis effect of hispolon from Phellinus linteus (Berkeley & Curtis) Teng on human epidermoid KB cells.

    Science.gov (United States)

    Chen, Wei; He, Fei-Yu; Li, Yong-Quan

    2006-04-21

    Phellinus linteus (Berkeley & Curtis) Teng, a well-known fungus of the genus Phellinus in the family of Hymenochaetaceae, is being increasingly used to treat a wide variety of disease processes such as oral ulcer, gastroenteric disorder, inflammation, lymphatic disease, and various cancers. However, the mechanism underlying its anti-oral cancer effect is poorly understood. In the present study, we prepared the ethanol extract of Phellinus linteus as a crude drug, and then obtained the active component hispolon by bioassay-guided isolation. Hispolon showed a dose-dependent inhibition of human epidermoid KB cell proliferation with IC50 of 4.62+/-0.16 microg/ml. Furthermore, it was revealed that hispolon could induce human epidermoid KB cell apoptosis with the characteristic of a DNA ladder, and with a significant increase of sub-G1. This process was accompanied by the collapse of mitochondrial membrane potential, the release of cytochrome c and the activation of Caspase-3. These results demonstrated that hispolon induced the death of KB cells through a mitochondria mediated apoptotic pathway. We propose that Phellinus linteus and its effective components could be used as an anti-oral cancer drug for future studies.

  1. Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3

    Directory of Open Access Journals (Sweden)

    Kumar Pranaw

    2014-01-01

    Full Text Available Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth were optimized by rotatable central composite design (RCCD using response surface methodology (RSM. The effects of all five chemical components (considered as independent variables, namely tryptone, soya peptone, dextrose, NaCl, and dipotassium phosphate, on protease production (dependent variable were studied, and it was found that tryptone and dextrose had maximum influence on protease production. The protease production was increased significantly by 66.31% under optimal medium conditions (tryptone—5.71, soya peptone—4.9, dextrose—1.45, NaCl—6.08, and dipotassium phosphate—0.47 in g/L. To best of knowledge, there are no reports on optimization of medium component for protease production by X. indica KB-3 using RSM and their application in fibrinolysis. This study will be useful for industrial processes for production of protease enzyme from X. indica KB-3 for its application in the field of agriculture and medicine.

  2. Nitric oxide scavenging by red cell microparticles.

    Science.gov (United States)

    Liu, Chen; Zhao, Weixin; Christ, George J; Gladwin, Mark T; Kim-Shapiro, Daniel B

    2013-12-01

    Red cell microparticles form during the storage of red blood cells and in diseases associated with red cell breakdown and asplenia, including hemolytic anemias such as sickle cell disease. These small phospholipid vesicles that are derived from red blood cells have been implicated in the pathogenesis of transfusion of aged stored blood and hemolytic diseases, via activation of the hemostatic system and effects on nitric oxide (NO) bioavailability. Red cell microparticles react with the important signaling molecule NO almost as fast as cell-free hemoglobin, about 1000 times faster than red-cell-encapsulated hemoglobin. The degree to which this fast reaction with NO by red cell microparticles influences NO bioavailability depends on several factors that are explored here. In the context of stored blood preserved in ADSOL, we find that both cell-free hemoglobin and red cell microparticles increase as a function of duration of storage, and the proportion of extra erythrocytic hemoglobin in the red cell microparticle fraction is about 20% throughout storage. Normalized by hemoglobin concentration, the NO-scavenging ability of cell-free hemoglobin is slightly higher than that of red cell microparticles as determined by a chemiluminescence NO-scavenging assay. Computational simulations show that the degree to which red cell microparticles scavenge NO will depend substantially on whether they enter the cell-free zone next to the endothelial cells. Single-microvessel myography experiments performed under laminar flow conditions demonstrate that microparticles significantly enter the cell-free zone and inhibit acetylcholine, endothelial-dependent, and NO-dependent vasodilation. Taken together, these data suggest that as little as 5 μM hemoglobin in red cell microparticles, an amount formed after the infusion of one unit of aged stored packed red blood cells, has the potential to reduce NO bioavailability and impair endothelial-dependent vasodilation.

  3. RED HOUSE

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    <正> 在Jimi Hendrix的这首《Red House》中,我们可以领略到非常多紧密、连贯、经典的吉他乐句,尤其是乐曲中众多给人以胁迫感的乐句更是另人回味无常。 在我们学习这首《Red House》的时候,就像学习Jimi Hendrix的其他曲目一样,最有效的办法就是先用心聆听几遍这首歌曲,然后再拿起吉他跟随着原曲一起反复弹奏。无论何时学习Jimi的歌曲,我们一定要意识到

  4. Red Tour

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Red Tour, the tourism uniquely to enjoy in China, is not only the landscapes to appreciate, but also the text book by means of which to learn the Chinese Revolutions from the birth of the China Communist Partyto the founding of the P. R. China. Itisalso a window, through which the foreign friends who are eager to learn the Chinese Revolutionary History could be very satired.

  5. Red Tour

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

      Red Tour, the tourism uniquely to enjoy in China, is not only the landscapes to appreciate, but also the text book by means of which to learn the Chinese Revolutions from the birth of the China Communist Partyto the founding of the P. R. China. Itisalso a window, through which the foreign friends who are eager to learn the Chinese Revolutionary History could be very satired.……

  6. Third harmonic generation in LiKB{sub 4}O{sub 7} single crystal

    Energy Technology Data Exchange (ETDEWEB)

    Kulyk, B., E-mail: bohdan_kulyk@yahoo.com [Scientific-Technical and Educational Center of Low Temperature Studies, Chair of Solid State Physics, Ivan Franko National University of Lviv, 50 Dragomanova Str., Lviv 79005 (Ukraine); Kapustianyk, V. [Scientific-Technical and Educational Center of Low Temperature Studies, Chair of Solid State Physics, Ivan Franko National University of Lviv, 50 Dragomanova Str., Lviv 79005 (Ukraine); Burak, Ya.; Adamiv, V. [Institute of Physical Optics, 23 Dragomanova Str., Lviv 79005 (Ukraine); Sahraoui, B. [POMA Laboratory, FRE CNRS 2988 UFR Sciences, University of Angers, 2 Boulevard Lavoisier, 49045 Angers Cedex 01 (France)

    2010-03-15

    The third order nonlinear optical properties of the lithium potassium borate (LiKB{sub 4}O{sub 7}) single crystal have been investigated by means of the rotational Maker fringe technique using Nd:YAG laser at 1064 nm working in picosecond regime. The value of the third order nonlinear optical susceptibility was calculated using the theoretical model of Kajzar et al. and was found to be about 1.4 x 10{sup -21} m{sup 2} V{sup -2} that is one order higher than that of fused silica.

  7. Genome Sequence of the Endophytic Bacterium Bacillus thuringiensis Strain KB1, a Potential Biocontrol Agent against Phytopathogens

    OpenAIRE

    Jeong, Haeyoung; Jo, Sung Hee; Hong, Chi Eun; Park, Jeong Mee

    2016-01-01

    Bacillus thuringiensis is the most widely known microbial pesticide used in agricultural applications. Herein, we report a draft genome sequence of the endophytic bacterium Bacillus thuringiensis strain KB1, which exhibits antagonism against phytopathogens.

  8. Genome Sequence of the Endophytic Bacterium Bacillus thuringiensis Strain KB1, a Potential Biocontrol Agent against Phytopathogens.

    Science.gov (United States)

    Jeong, Haeyoung; Jo, Sung Hee; Hong, Chi Eun; Park, Jeong Mee

    2016-04-21

    ITALIC! Bacillus thuringiensisis the most widely known microbial pesticide used in agricultural applications. Herein, we report a draft genome sequence of the endophytic bacterium ITALIC! Bacillus thuringiensisstrain KB1, which exhibits antagonism against phytopathogens.

  9. Cross-check of ex-situ and in-situ metrology of a bendable temperature stabilized KB mirror

    Energy Technology Data Exchange (ETDEWEB)

    Yuan, Sheng Sam; Goldberg, Kenneth A.; Yashchuk, Valeriy V.; Celestre, Richard; McKinney, Wayne R.; Morrison, Gregory; Macdougall, James; Mochi, Iacopo; Warwick, Tony

    2010-09-15

    At the Advanced Light Source (ALS), we are developing broadly applicable, high-accuracy, in-situ, at-wavelength wavefront slope measurement techniques for Kirkpatrick-Baez (KB) mirror nano-focusing. In this paper, we report an initial cross-check of ex-situ and in-situ metrology of a bendable temperature stabilized KB mirror. This cross-check provides a validation of the in-situ shearing interferometry currently under development at the ALS.

  10. YQ36: A Novel Bisindolylmaleimide Analogue Induces KB/VCR Cell Death

    Directory of Open Access Journals (Sweden)

    Ji Cao

    2009-01-01

    Full Text Available Overexpression of multidrug resistance proteins P-glycoprotein (P-gp, MDR1 causes resistance of the tumor cells against a variety of chemotherapeutic agents. 3-(1-methyl-1H-indol-3-yl-1-phenyl-4-(1-(3-(piperidin-1-ylpropyl-1H-pyrazolo[3,4-b]pyridine-3-yl-1H-pyrrole-2,5-dione (YQ36 is a novel analogue of bisindolylmaleimide, which has been reported to overcome multidrug resistance. Here, we dedicated to investigate the anticancer activity of YQ36 on KB/VCR cells. The results revealed that YQ36 exhibited great antiproliferative activity on three parental cell lines and MDR1 overexpressed cell lines. Moreover, the hypersensitivity of YQ36 was confirmed on the base of great apoptosis induction and unaltered intracellular drug accumulation in KB/VCR cells. Further results suggested that YQ36 could not be considered as a substrate of P-gp, which contributed to its successfully escaping from the efflux mediated by P-gp. Interestingly, we observed that YQ36 could accumulate in nucleus and induce DNA damage. YQ36 could also induce the activation of caspase-3, imposing effects on the mitochondrial function. Collectively, our data demonstrated that YQ36 exhibited potent activities against MDR cells, inducing DNA damage and triggering subsequent apoptosis via mitochondrial pathway.

  11. A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion

    Institute of Scientific and Technical Information of China (English)

    Mika Hayashi-Tsugane; Masahiko Maekawa; Qian Qian; Hirokazu Kobayashi; Shigeru Iida; Kazuo Tsugane

    2011-01-01

    We have isolated a recessive rice mutant,designated as indeterminate growth(ing),which displays creeping and apparent heterochronic phenotypes in the vegetative period with lanky and winding culms.Rough mapping and subsequent molecular characterization revealed that the ing mutant carries a large deletion,which corresponds to a 103 kb region in the Nipponbare genome,containing nine annotated genes on chromosome 3.Of these annotated genes,the SLRI gene encoding a DELLA protein is the only one that is well characterized in its function,and its null mutation,which is caused by a single base deletion in the middle of the intronless SLR1 gene,confers a slender phenotype that bears close resemblance to the ing mutant phenotype.The primary cause of the ing mutant phenotype is the deletion of the SLR1 gene,and the ing mutant appears to be the first characterized mutant having the entire SLRI sequence deleted.Our results also suggest that the deleted region of 103 kb does not contain an indispensable gene,whose dysfunction must result in a lethal phenotype.

  12. Prediction of Metabolic Pathway Involvement in Prokaryotic UniProtKB Data by Association Rule Mining

    KAUST Repository

    Boudellioua, Imane

    2016-07-08

    The widening gap between known proteins and their functions has encouraged the development of methods to automatically infer annotations. Automatic functional annotation of proteins is expected to meet the conflicting requirements of maximizing annotation coverage, while minimizing erroneous functional assignments. This trade-off imposes a great challenge in designing intelligent systems to tackle the problem of automatic protein annotation. In this work, we present a system that utilizes rule mining techniques to predict metabolic pathways in prokaryotes. The resulting knowledge represents predictive models that assign pathway involvement to UniProtKB entries. We carried out an evaluation study of our system performance using cross-validation technique. We found that it achieved very promising results in pathway identification with an F1-measure of 0.982 and an AUC of 0.987. Our prediction models were then successfully applied to 6.2 million UniProtKB/TrEMBL reference proteome entries of prokaryotes. As a result, 663,724 entries were covered, where 436,510 of them lacked any previous pathway annotations.

  13. VenomKB, a new knowledge base for facilitating the validation of putative venom therapies.

    Science.gov (United States)

    Romano, Joseph D; Tatonetti, Nicholas P

    2015-11-24

    Animal venoms have been used for therapeutic purposes since the dawn of recorded history. Only a small fraction, however, have been tested for pharmaceutical utility. Modern computational methods enable the systematic exploration of novel therapeutic uses for venom compounds. Unfortunately, there is currently no comprehensive resource describing the clinical effects of venoms to support this computational analysis. We present VenomKB, a new publicly accessible knowledge base and website that aims to act as a repository for emerging and putative venom therapies. Presently, it consists of three database tables: (1) Manually curated records of putative venom therapies supported by scientific literature, (2) automatically parsed MEDLINE articles describing compounds that may be venom derived, and their effects on the human body, and (3) automatically retrieved records from the new Semantic Medline resource that describe the effects of venom compounds on mammalian anatomy. Data from VenomKB may be selectively retrieved in a variety of popular data formats, are open-source, and will be continually updated as venom therapies become better understood.

  14. Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48

    Directory of Open Access Journals (Sweden)

    Mohd Zabidi Majid

    2014-01-01

    Full Text Available Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation. This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their mode of cell death. The concentration producing 50% cell inhibition (IC50 was determined and the activity was examined under an inverted microscope. Immunohistochemistry fluorescent staining method (TUNEL was performed to indicate the mechanism of cell death and the fragmented DNA band pattern produced was obtained for verification. Compared to Azadirachta sp. and Typhonium sp., the antiproliferative activity of Brucea sp. extract was the most potent on both KB and ORL-48 cells with IC50 of 24.37 ± 1.75 and 6.67 ± 1.15 µg/mL, respectively. Signs of cell attrition were observed 24 hr after treatment. Green fluorescent spots indicating cell death by apoptosis were observed in images of both cells following treatment with all the three extracts. DNA fragments harvested from Brucea-treated cells produced bands in a ladder pattern suggesting the apoptotic effect of the extract. It is thus concluded that Brucea sp. extract exhibited cytotoxic activity on ORL-48 cells and their action mechanism is via apoptosis.

  15. Sixteen additional enhancers associated with the chicken Sox2 locus outside the central 50-kb region.

    Science.gov (United States)

    Okamoto, Ryuji; Uchikawa, Masanori; Kondoh, Hisato

    2015-01-01

    The transcription factor Sox2 plays a central role in the regulation of neuro-sensory development, and many other developmental processes. To gain an in depth understanding of the Sox2 gene regulation, we previously investigated the Sox2-proximal 50-kb region of the chicken genome to determine enhancers based on functional assays using chicken embryo electroporation. We identified 11 enhancers with specificity for neuro-sensory tissues. In this study, we extended the analysis of Sox2 locus-associated enhancers to a 200-kb region and identified 16 additional enhancers with functions in neuro-sensory development. These enhancers roughly correspond to a fraction of the sequence blocks that are highly conserved between chicken and mammalian genomes. The neural enhancers were activated in sequence, thereby creating a complex pattern of functional overlaps in the developing central nervous system (CNS). The variations in the specificities of the sensory enhancers also reflected the intermediate steps of sensory tissue development. This study provides an example where a single transcription factor gene has numerous regulatory elements that allow it to fulfill many functional roles in different biological contexts.

  16. Structural and optical properties of LiKB{sub 4}O{sub 7} single crystals grown by Czochralski technique

    Energy Technology Data Exchange (ETDEWEB)

    Sukumar, M. [Crystal Growth and Thin Film Laboratory, Department of Physics, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu (India); Babu, R. Ramesh, E-mail: rampap2k@yahoo.co.in [Crystal Growth and Thin Film Laboratory, Department of Physics, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu (India); Ramamurthi, K. [Crystal Growth and Thin Film Laboratory, Department of Physics and Nanotechnology, Faculty of Engineering and Technology, SRM University, Kattankulathur 603 203, Tamil Nadu (India); Bhagavannarayana, G. [Crystal Growth and Crystallography Section, National Physical Laboratory, Krishnan Marg, New Delhi 110 012 (India)

    2015-06-15

    One of the alkali metal borates, lithium potassium borate (LiKB{sub 4}O{sub 7}) single crystal, was grown following two different micro step pulling movements employing the modified crystal puller. The influence of two different micro step pulling movements on the crystalline nature, optical properties and micro morphology of the grown LiKB{sub 4}O{sub 7} crystal was investigated by high resolution X-ray diffraction (HRXRD) analysis and birefringence interferometry and chemical etching techniques, respectively. HRXRD studies revealed that the crystalline perfection of the grown crystals is reasonably good. Interferometric images showed that the crystal grown under higher micro step pulling movement has very less number of scattering centers. The etching studies revealed that the crystal grown under higher micro steps pulling movement contains relatively low level dislocation density. - Graphical abstract: Diffraction curve recorded for LiKB{sub 4}O{sub 7} crystal from (a) top portion and (b) bottom portion. - Highlights: • LiKB{sub 4}O{sub 7} crystal was grown under two different micro stepping movements by the crystal puller. • Crystalline nature, optical properties and micro morphology of LiKB{sub 4}O{sub 7} were investigated. • The micro stepping pull movement reduces the dislocation density during the growth of LiKB{sub 4}O{sub 7} crystals.

  17. Disease Progression Patterns of SHIV-KB9 in Rhesus Macaques of Chinese Origin in Comparison with Indian Macaques

    Institute of Scientific and Technical Information of China (English)

    QIANG LIU; GUI-BO YANG; HUI ZHAO; QIANG WEI; HUI XING; CHUAN QIN; YI-MING SHAO

    2008-01-01

    To develop a model of SHIV-KB9/Chinese origin rhesus (Ch Rh) macaques for vaccine research and to compare the pathogenesis of SHIV-KB9 in Ch Rh macaques with that reported in Indian rhesus (had Rh) macaques. Methods Seven mamu-A*01 negative Ch Rh macaques were inoculated intravenously with 1-10000 MID of SHIV-KB9. The monkeys were monitored for viral load, CD4, CD8, SHIV-specific antibody and virus genetic variation. The results were compared with those previously observed in Ind Rh macaques. Results As compared to that observed in Ind Rh macaques, SHIV-KB9 in Ch Rh macaques displayed three identical disease progression patterns. However, the primary pattern was not identical between the two subspecies. The level of plasma viremia differed in SHIV-KB9-infected Ch Rh macaques which exhibited different outcomes from those in Ind Rh macaques. Generally, the values of viral load and the maintenance of CD4 T cells were associated with humoral responses. Otherwise, the viral genetic distances (divergence, diversity) were larger in animals (M419, M425) with their CD4T cells profoundly depleted. Conclusion The model of SHIV-KB9/Ch Rh macaques displays a relatively slow progression to AIDS compared with Ind Rh macaques, which may more accurately reflect the potential ofcandidate vaccines in humans.

  18. The Red Capitalist's Red Face

    Institute of Scientific and Technical Information of China (English)

    ROGER CHEUNG

    2008-01-01

    @@ A disastrous derivatives investment has seen Citic Pacific chairman Rong Zhijian lose hundreds of millions of dollars - not to mention his reputation Rong Zhijian may well go down in the history books as the last of China's "Red Capitalists," a group of pre-revolutionary tycoons who stayed on post-1949. His family owned one of China's largest private enterprises before 1949 and his father, Rong Yiren, who served as China's vice-president from 1993 to 1998, was hand-picked by Deng Xiaoping to oversee China's economic opening and reform during the 1980s.

  19. Exact break point of a 50 kb deletion 8 kb centromeric of the HLA-A locus with HLA-A*24:02: the same deletion observed in other A*24 alleles and A*23:01 allele.

    Science.gov (United States)

    Mitsunaga, Shigeki; Okudaira, Yuko; Kunii, Nanae; Cui, Tailin; Hosomichi, Kazuyoshi; Oka, Akira; Suzuki, Yasuo; Homma, Yasuhiko; Sato, Shinji; Inoue, Ituro; Inoko, Hidetoshi

    2011-08-01

    In a structural aberration analysis of patients with arthritis mutilans, a 50 kb deletion near the HLA-A locus with HLA-A*24:02 allele was detected. It was previously reported that HLA-A*24:02 haplotype harbored a large-scale deletion telomeric of the HLA-A gene in healthy individuals. In order to confirm that the deletion are the same in patients with arthritis mutilans and in healthy individuals, and to identify the break point of this deletion, the boundary sequences across the deletion in A*24:02 was amplified by polymerase chain reaction (PCR) as a 3.7 kb genomic fragment and subjected to nucleotide sequence determination. A comparison of these genomic sequences with those of the non-A*24:02 haplotype revealed that the deleted genomic region spanning 50 kb was flanked by 3.7 kb repetitive element-rich segments homologous to each other on both sides in non-A*24. The nucleotide sequences of the PCR products were identical in patients with arthritis mutilans and in healthy individuals, revealing that the deletion linked to A*24:02 is irrelevant to the onset of arthritis mutilans. The deletion was detected in all other A*24 alleles so far examined but not in other HLA-A alleles, except A*23:01. This finding, along with the phylogenic tree of HLA-A alleles and the presence of the 3.7 kb highly homologous segments at the boundary of the deleted genomic region in A*03 and A*32, may suggest that this HLA-A*24:02-linked deletion was generated by homologous recombination within two 3.7 kb homologous segments situated 50 kb apart in the ancestral A*24 haplotype after divergence from the A*03 and A*32 haplotypes.

  20. Loss of retrovirus production in JB/RH melanoma cells transfected with H-2Kb and TAP-1 genes.

    Science.gov (United States)

    Li, M; Xu, F; Muller, J; Huang, X; Hearing, V J; Gorelik, E

    1999-01-20

    JB/RH1 melanoma cells, as well as other melanomas of C57BL/6 mice (B16 and JB/MS), express a common melanoma-associated antigen (MAA) encoded by an ecotropic melanoma-associated retrovirus (MelARV). JB/RH1 cells do not express the H-2Kb molecules due to down-regulation of the H-2Kb and TAP-1 genes. When JB/RH1 cells were transfected with the H-2Kb and cotransfected with the TAP-1 gene, it resulted in the appearance of H-2Kb molecules and an increase in their immunogenicity, albeit they lost expression of retrovirus-encoded MAA recognized by MM2-9B6 mAb. Loss of MAA was found to result from a complete and stable elimination of ecotropic MelARV production in the H-2Kb/TAP-1-transfected JB/RH1 cells. Northern blot analysis showed no differences in ecotropic retroviral messages in MelARV-producing and -nonproducing melanoma cells, suggesting that loss of MelARV production was not due to down-regulation of MelARV transcription. Southern blot analysis revealed several rearrangements in the proviral DNA of H-2Kb-positive JB/RH1 melanoma cells. Sequence analysis of the ecotropic proviral DNA from these cells showed numerous nucleotide substitutions, some of which resulted in the appearance of a novel intraviral PstI restriction site and the loss of a HindIII restriction site in the pol region. PCR amplification of the proviral DNAs indicates that an ecotropic provirus found in the H-2Kb-positive cells is novel and does not preexist in the parental H-2Kb-negative melanoma cells. Conversely, the ecotropic provirus of the parental JB/RH1 cells was not amplifable from the H-2Kb-positive cells. Our data indicate that stable loss of retroviral production in the H-2Kb/TAP-1-transfected melanoma cells is probably due to the induction of recombination between a productive ecotropic MelARV and a defective nonecotropic provirus leading to the generation of a defective ecotropic provirus and the loss of MelARV production and expression of the retrovirus-encoded MAA. Copyright 1999

  1. Narrowing the Duane syndrome critical region at chromosome 8q13 down to 40 kb.

    Science.gov (United States)

    Calabrese, G; Telvi, L; Capodiferro, F; Morizio, E; Pizzuti, A; Stuppia, L; Bordoni, R; Ion, A; Fantasia, D; Mingarelli, R; Palka, G

    2000-05-01

    Duane syndrome (MIM 126800) is an autosomal dominant disorder characterised by primary strabismus and other ocular anomalies, associated with variable deficiency of binocular sight. We have recently identified a syndrome carrying a reciprocal translation t(6;8)(q26;q13). FISH and PCR analyses using a YAC contig spanning the SRO narrowed the Duane region to a < 1 cM interval between markers SHGC37325 and W14901. In addition, the identification and mapping of two PAC clones flanking the translocation breakpoint, allowed us to further narrow the critical region to about 40 kb. As part of these mapping studies, we have also refined the map position of AMYB, a putative candidate gene, to 8q13, centromeric to Duane locus. AMYB is expressed in brain cortex and genital crests and has been previously mapped to 8q22.

  2. A hard x-ray KB-FZP microscope for tomography with sub-100-nm resolution

    Energy Technology Data Exchange (ETDEWEB)

    Rau, C.; Crecea, V.; Richter, C.-P.; Peterson, K.M.; Jemian, P.R.; Neuhausler, U.; Schneider, G.; Yu, X.; Braun, P.V.; Chiang, T.-C.; Robinson, I.K. (UIUC); (NWU); (Bessy); (U Bielefeld)

    2008-08-04

    An instrument for high-resolution imaging and tomography has been built at the APS beamline 34 ID-C, Argonne National Laboratory. In-line phase contrast tomography can be performed with micrometer resolution. For imaging and tomography with resolution better than 100nm a hard X-ray microscope has been integrated to the instrument. It works with a Kirkpatrick-Baez (KB) mirror as condenser and a Fresnel-Zone plate (FZP) as an objective lens. 50 nm-features have been resolved in a Nickel structure operating the microscope at a photon energy of 9keV. Phase objects with negligible absorption contrast have been imaged. Tomography scans were performed on photonic crystals.

  3. Pro-Dopamine Regulator - (KB220) to Balance Brain Reward Circuitry in Reward Deficiency Syndrome (RDS).

    Science.gov (United States)

    Blum, Kenneth; Febo, Marcelo; Fried, Lyle; Baron, David; Braverman, Eric R; Dushaj, Kristina; Li, Mona; Demetrovics, Zsolt; Badgaiyan, Rajendra D

    2017-01-01

    We are faced with a worldwide opiate/opioid epidemic that is devastating. According to the Centers for Disease Control and Prevention (CDC), at least 127 people, young and old, are dying every day in America due to narcotic overdose. The Food and Drug Administration (FDA) has approved Medication-Assisted Treatments (MATs) for opiate/opioids as well as alcohol and nicotine. The mechanism of action of most MATS favors either blocking of dopaminergic function or a form of Opiate Substitution Therapy (OST). These treatment options are adequate for short-term treatment of the symptoms of addiction and harm reduction but fail long-term to deal with the cause or lead to recovery. There is a need to continue to seek better treatment options. This mini-review is the history of the development of one such treatment; a glutaminergic-dopaminergic optimization complex called KB220. Growing evidence indicates that brain reward circuitry controls drug addiction, in conjunction with "anti-reward systems" as the "anti-reward systems" can be affected by both glutaminergic and dopaminergic transmission. KB220 may likely alter the function of these regions and provide for the possible eventual balancing the brain reward system and the induction of "dopamine homeostasis." Many of these concepts have been reported elsewhere and have become an integral part of the addiction science literature. However, the concise review may encourage readership to reconsider these facts and stimulate further research focused on the impact that the induction of "dopamine homeostasis" may have on recovery and relapse prevention.

  4. Red blood cell production

    Science.gov (United States)

    ... to one part of the body or another. Red blood cells are an important element of blood. Their job ... is carried to and eliminated by the lungs. Red blood cells are formed in the red bone marrow of ...

  5. Changes of NF-KB activity in colon carcinoma cells treated with different crude extracts of abrotani herba

    Institute of Scientific and Technical Information of China (English)

    Feng Pan; Yuying Chen; Li Yang; Zhiheng Bian; Houjie Liang

    2008-01-01

    Objective: To study changes of NF-KB activity in colon carcinoma cell lines treated with different crude extracts of abrotani herba obtained through solvent extraction methods.Methods: Crude extracts of abrotani herba were extracted with ligarine, chloroform, acetoacetate and n-butanol in separating funnel.Exposure concentration of crude extracts were obtained through detecting viability of HT-29 cells by MTT.Then HT-29 cells and Lovo cells were treated with different crude extracts respectively.Changes of NF-KB activity in HT-29 cells and Lovo cells using different crude extracts were observed by EMSA.Results: Successfully extracted different crude extracts of abrotani herba and called them ligarine extract, chloroform extract,acetoacetate extract, n-butanol extract and remaining extract for short.NF-KB activity was significantly inhibited in HT-29 cells treated with chloroform extract, there were no significant differences in other groups compared with the control.The same change of NF-KB activity was observed in Lovo calls using different crude extracts of abrotani herba.Conclusion: NF-KB activity can be inhibited in colon carcinoma HT-29 calls and Lovo cells treated with chloroform extract obtained from abrotani herba through the method of solvent extraction.

  6. Interferon alpha2 recombinant and epidermal growth factor modulate proliferation and hypusine synthesis in human epidermoid cancer KB cells.

    Science.gov (United States)

    Caraglia, M; Passeggio, A; Beninati, S; Leardi, A; Nicolini, L; Improta, S; Pinto, A; Bianco, A R; Tagliaferri, P; Abbruzzese, A

    1997-06-15

    We previously found that interferon alpha2 recombinant (IFNalpha) increases the expression of epidermal growth factor receptor (EGF-R) in the human epidermoid cancer KB cell line. Here we report the effects of IFNalpha and epidermal growth factor (EGF) on KB cell cycle kinetics. IFNalpha (1000 i.u./ml) for 48 h decreased the S-phase fraction and diminished the expression of Ki67 and proliferating cell nuclear antigen on KB cells. Incubation of IFNalpha-treated KB cells with 10 nM EGF for 12 h reversed these effects. We then studied several biochemical markers of cell proliferation. Ornithine decarboxylase activity was decreased to about one-tenth by IFNalpha and partly restored by EGF. Hypusine is contained only in eukaryotic initiation factor 5A and its levels are correlated with cell proliferation. IFNalpha decreased hypusine synthesis by 75%; exposure of cells to EGF for 12 h restored hypusine synthesis almost completely. We also studied the effects of IFNalpha on the cytotoxicity of the recombinant toxin TP40, which inhibits elongation factor 2 through EGF-R binding and internalization. IFNalpha greatly enhanced the TP40-induced inhibition of protein synthesis in KB cells. In conclusion, IFNalpha, which affects protein synthesis machinery and increases EGF-R expression, enhances the tumoricidal activity of TP40 and hence could be useful in the setting of anti-cancer therapy.

  7. Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome.

    Directory of Open Access Journals (Sweden)

    Lisa M Nigro

    2016-08-01

    Full Text Available Deep-sea hypersaline anoxic basins (DHABs and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that has been consistently observed in hypersaline microbial diversity studies. Here we report the phylogeography of KB1, its phylogenetic relationships to Candidate Division OP1 Bacteria, and its potential metabolic and osmotic stress adaptations based on a partial single cell amplified genome (SAG of KB1 from Orca Basin, the largest hypersaline seafloor brine basin in the Gulf of Mexico. Our results are consistent with the hypothesis – previously developed based on 14C incorporation experiments with mixed-species enrichments from Mediterranean seafloor brines - that KB1 has adapted its proteins to elevated intracellular salinity, but at the same time KB1 apparently imports glycine betaine; this compatible solute is potentially not limited to osmoregulation but could also serve as a carbon and energy source.

  8. rsfMRI effects of KB220Z™ on Neural Pathways in Reward Circuitry of Abstinent Genotyped Heroin Addicts

    Science.gov (United States)

    Blum, Kenneth; Liu, Yijun; Wang, Wei; Wang, Yarong; Zhang, Yi; Oscar-Berman, Marlene; Smolen, Andrew; Febo, Marcelo; Han, David; Simpatico, Thomas; Cronjé, Frans J; Demetrovics, Zsolt; Gold, Mark S.

    2016-01-01

    Recently Willuhn et al. reported that cocaine use and even non-substance related addictive behavior, increases, as dopaminergic function is reduced. Chronic cocaine exposure has been associated with decreases in D2/D3 receptors, also associated with lower activation to cues in occipital cortex and cerebellum in a recent PET study from Volkow’s group. Therefore, treatment strategies, like dopamine agonist therapy, that might conserve dopamine function may be an interesting approach to relapse prevention in psychoactive drug and behavioral addictions. To this aim, we evaluated the effect of KB220Z™ on reward circuitry of ten heroin addicts undergoing protracted abstinence, an average 16.9 months. In a randomized placebo-controlled crossover study of KB220Z™ five subjects completed a triple blinded–experiment in which the subject, the person administering the treatment and the person evaluating the response to treatment were blinded as to which treatment any particular subject was receiving. In addition, nine subjects total were genotyped utilizing the GARSRX™ test. We preliminarily report that KB220Z ™ induced an increase in BOLD activation in caudate-accumbens-dopaminergic pathways compared to placebo following one-hour acute administration. Furthermore, KB220Z™ also reduced resting state activity in the putamen of abstinent heroin addicts. In the second phase of this pilot study of all ten abstinent heroin-dependent subjects, three brain regions of interest (ROIs) we observed to be significantly activated from resting state by KB220Z compared to placebo (P addiction by direct or indirect dopaminergic interaction. Due to small sample size, we caution definitive interpretation of these preliminary results and confirmation with additional research and ongoing rodent and human studies of KB220Z, is required. PMID:25526228

  9. Construction and utility of 10-kb libraries for efficient clone-gap closure for rice genome sequencing.

    Science.gov (United States)

    Yang, Tae-Jin; Yu, Yeisoo; Nah, Gyoungju; Atkins, Michael; Lee, Seunghee; Frisch, David A; Wing, Rod A

    2003-08-01

    Rice is an important crop and a model system for monocot genomics, and is a target for whole genome sequencing by the International Rice Genome Sequencing Project (IRGSP). The IRGSP is using a clone by clone approach to sequence rice based on minimum tiles of BAC or PAC clones. For chromosomes 10 and 3 we are using an integrated physical map based on two fingerprinted and end-sequenced BAC libraries to identifying a minimum tiling path of clones. In this study we constructed and tested two rice genomic libraries with an average insert size of 10 kb (10-kb library) to support the gap closure and finishing phases of the rice genome sequencing project. The HaeIII library contains 166,752 clones covering approximately 4.6x rice genome equivalents with an average insert size of 10.5 kb. The Sau3AI library contains 138,960 clones covering 4.2x genome equivalents with an average insert size of 11.6 kb. Both libraries were gridded in duplicate onto 11 high-density filters in a 5 x 5 pattern to facilitate screening by hybridization. The libraries contain an unbiased coverage of the rice genome with less than 5% contamination by clones containing organelle DNA or no insert. An efficient method was developed, consisting of pooled overgo hybridization, the selection of 10-kb gap spanning clones using end sequences, transposon sequencing and utilization of in silico draft sequence, to close relatively small gaps between sequenced BAC clones. Using this method we were able to close a majority of the gaps (up to approximately 50 kb) identified during the finishing phase of chromosome-10 sequencing. This method represents a useful way to close clone gaps and thus to complete the entire rice genome.

  10. Transcriptional regulation of mouse alpha A-crystallin gene in a 148kb Cryaa BAC and its derivates

    Directory of Open Access Journals (Sweden)

    Yang Ying

    2008-09-01

    Full Text Available Abstract Background αA-crystallin is highly expressed in the embryonic, neonatal and adult mouse lens. Previously, we identified two novel distal control regions, DCR1 and DCR3. DCR1 was required for transgenic expression of enhanced green fluorescent protein, EGFP, in lens epithelium, whereas DCR3 was active during "late" stages of lens primary fiber cell differentiation. However, the onset of transgenic EGFP expression was delayed by 12–24 hours, compared to the expression of the endogenous Cryaa gene. Results Here, we used bacterial artificial chromosome (BAC and standard transgenic approaches to examine temporal and spatial regulation of the mouse Cryaa gene. Two BAC transgenes, with EGFP insertions into the third coding exon of Cryaa gene, were created: the intact αA-crystallin 148 kb BAC (αA-BAC and αA-BAC(ΔDCR3, which lacks approximately 1.0 kb of genomic DNA including DCR3. Expression of EGFP in the majority of both BAC transgenics nearly recapitulated the endogenous expression pattern of the Cryaa gene in lens, but not outside of the lens. The number of cells expressing αA-crystallin in the lens pit was higher compared to the number of cells expressing EGFP. Next, we generated additional lines using a 15 kb fragment of αA-crystallin locus derived from αA-BAC(ΔDCR3, 15 kb Cryaa/EGFP. A 15 kb region of Cryaa/EGFP supported the expression pattern of EGFP also in the lens pit. However, co-localization studies of αA-crystallin and EGFP indicated that the number of cells that showed transgenic expression was higher compared to cells expressing αA-crystallin in the lens pit. Conclusion We conclude that a 148 kb αA-BAC likely contains all of the regulatory regions required for αA-crystallin expression in the lens, but not in retina, spleen and thymus. In addition, while the 15 kb Cryaa/EGFP region also supported the expression of EGFP in the lens pit, expression in regions such as the hindbrain, indicate that additional genomic

  11. PGen: large-scale genomic variations analysis workflow and browser in SoyKB.

    Science.gov (United States)

    Liu, Yang; Khan, Saad M; Wang, Juexin; Rynge, Mats; Zhang, Yuanxun; Zeng, Shuai; Chen, Shiyuan; Maldonado Dos Santos, Joao V; Valliyodan, Babu; Calyam, Prasad P; Merchant, Nirav; Nguyen, Henry T; Xu, Dong; Joshi, Trupti

    2016-10-06

    With the advances in next-generation sequencing (NGS) technology and significant reductions in sequencing costs, it is now possible to sequence large collections of germplasm in crops for detecting genome-scale genetic variations and to apply the knowledge towards improvements in traits. To efficiently facilitate large-scale NGS resequencing data analysis of genomic variations, we have developed "PGen", an integrated and optimized workflow using the Extreme Science and Engineering Discovery Environment (XSEDE) high-performance computing (HPC) virtual system, iPlant cloud data storage resources and Pegasus workflow management system (Pegasus-WMS). The workflow allows users to identify single nucleotide polymorphisms (SNPs) and insertion-deletions (indels), perform SNP annotations and conduct copy number variation analyses on multiple resequencing datasets in a user-friendly and seamless way. We have developed both a Linux version in GitHub ( https://github.com/pegasus-isi/PGen-GenomicVariations-Workflow ) and a web-based implementation of the PGen workflow integrated within the Soybean Knowledge Base (SoyKB), ( http://soykb.org/Pegasus/index.php ). Using PGen, we identified 10,218,140 single-nucleotide polymorphisms (SNPs) and 1,398,982 indels from analysis of 106 soybean lines sequenced at 15X coverage. 297,245 non-synonymous SNPs and 3330 copy number variation (CNV) regions were identified from this analysis. SNPs identified using PGen from additional soybean resequencing projects adding to 500+ soybean germplasm lines in total have been integrated. These SNPs are being utilized for trait improvement using genotype to phenotype prediction approaches developed in-house. In order to browse and access NGS data easily, we have also developed an NGS resequencing data browser ( http://soykb.org/NGS_Resequence/NGS_index.php ) within SoyKB to provide easy access to SNP and downstream analysis results for soybean researchers. PGen workflow has been optimized for the most

  12. American Red Cross

    Science.gov (United States)

    ... Know You’re Safe – Use Red Cross App and Website » Returning Home – Use Red Cross Flood Safety Steps » Red Cross Relief Efforts in the Caribbean & Mexico » At-a-Glance: Red Cross Response to Harvey, ...

  13. Analysis of gene and protein name synonyms in Entrez Gene and UniProtKB resources

    KAUST Repository

    Arkasosy, Basil

    2013-05-11

    Ambiguity in texts is a well-known problem: words can carry several meanings, and hence, can be read and interpreted differently. This is also true in the biological literature; names of biological concepts, such as genes and proteins, might be ambiguous, referring in some cases to more than one gene or one protein, or in others, to both genes and proteins at the same time. Public biological databases give a very useful insight about genes and proteins information, including their names. In this study, we made a thorough analysis of the nomenclatures of genes and proteins in two data sources and for six different species. We developed an automated process that parses, extracts, processes and stores information available in two major biological databases: Entrez Gene and UniProtKB. We analysed gene and protein synonyms, their types, frequencies, and the ambiguities within a species, in between data sources and cross-species. We found that at least 40% of the cross-species ambiguities are caused by names that are already ambiguous within the species. Our study shows that from the six species we analysed (Homo Sapiens, Mus Musculus, Arabidopsis Thaliana, Oryza Sativa, Bacillus Subtilis and Pseudomonas Fluorescens), rice (Oriza Sativa) has the best naming model in Entrez Gene database, with low ambiguities between data sources and cross-species.

  14. Identification and characterization of survivin-derived H-2Kb-restricted CTL epitopes

    DEFF Research Database (Denmark)

    Hofmann, Uta B; Voigt, Heike; Andersen, Mads H

    2009-01-01

    Survivin is overexpressed in several malignancies and in tumor-associated endothelium making it an attractive target for therapeutic cytotoxic T-cell responses. Thus, it would be important to test this notion in preclinical models. Consequently, we screened the murine survivin sequence for potent......Survivin is overexpressed in several malignancies and in tumor-associated endothelium making it an attractive target for therapeutic cytotoxic T-cell responses. Thus, it would be important to test this notion in preclinical models. Consequently, we screened the murine survivin sequence...... for potential binding K(b)-restricted octamer peptide epitopes. Two epitopes, which bind strongly to K(b), were selected to test their immunogenicity in vivo. Spleen cells from mice vaccinated by intradermal injection of mature DC pulsed with these peptides displayed reactivity to the respective epitopes....... The natural processing and presentation of these epitopes by tumor cells was evident by the killing of murine melanoma cells by vaccination-induced T cells. Subcutaneous challenge with syngeneic melanoma demonstrated the protective immunity of this vaccination. Notably, analysis of the vessel density...

  15. 苏云金芽孢杆菌伴孢晶体中20-kb DNAs上染色体基因的鉴定%Identification of Chromosomal Genes on 20-kb DNAs from Parasporal Crystal of Bacillus thuringiensis

    Institute of Scientific and Technical Information of China (English)

    孙运军; 袁志明; 夏立秋; 魏薇; 付祖姣; 黄璠; 丁学知; 胡胜标

    2007-01-01

    已有的研究表明苏云金芽孢杆菌(Bacillus thuringiensis,Bt)4.0718菌株菱形晶体中含有与原毒素紧密结合的20-kb DNAs,利用PCR-RFLP分析发现在这些20-kb DNAs上含有cry1Aa、cry1Ac、cry2Aa和cry2Ab基因.本研究利用特异引物从4.0718菌株中20-kb DNAs上分别扩增出带有自身调控序列的cry1Ac和cry2Aa基因,并将其分别电转至Bt无晶体菌株Cry-B中获得重组菌株Cry-B(pHC42)和Cry-B(pHC39).SDS-PAGE和透射电镜分析表明这两种重组菌株能够分别产生由130-kDa的Cry1Ac原毒素形成的菱形晶体以及由65-kDa的Cry2Aa原毒素形成的方形晶体.毒力生测的结果显示,这两种菌株的表达产物对棉铃虫(Helicoverpa armigera)幼虫具有预期的杀虫效果.重组菌株分别形成的两种晶体经不同的缓冲液溶解后能释放出与20-kb DNAs紧密结合的原毒素,同时在20-kb DNAs上均可检测到源于Bt染色体的spo0A和nprA基因片段.

  16. Single-stranded heteroduplex intermediates in λ Red homologous recombination

    Directory of Open Access Journals (Sweden)

    Zhang Youming

    2010-07-01

    Full Text Available Abstract Background The Red proteins of lambda phage mediate probably the simplest and most efficient homologous recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results Here we show that the Red proteins can act via full length single stranded intermediates to establish single stranded heteroduplexes at the replication fork. We created asymmetrically digestible dsDNA substrates by exploiting the fact that Redα exonuclease activity requires a 5' phosphorylated end, or is blocked by phosphothioates. Using these substrates, we found that the most efficient configuration for dsDNA recombination occurred when the strand that can prime Okazaki-like synthesis contained both homology regions on the same ssDNA molecule. Furthermore, we show that Red recombination requires replication of the target molecule. Conclusions Hence we propose a new model for dsDNA recombination, termed 'beta' recombination, based on the formation of ssDNA heteroduplexes at the replication fork. Implications of the model were tested using (i an in situ assay for recombination, which showed that recombination generated mixed wild type and recombinant colonies; and (ii the predicted asymmetries of the homology arms, which showed that recombination is more sensitive to non-homologies attached to 5' than 3' ends. Whereas beta recombination can generate deletions in target BACs of at least 50 kb at about the same efficiency as small deletions, the converse event of insertion is very sensitive to increasing size. Insertions up to 3 kb are most efficiently achieved using beta recombination, however at greater sizes, an alternative Red-mediated mechanism(s appears to be equally efficient. These findings define a new intermediate in homologous recombination, which also has practical implications for recombineering with the Red proteins.

  17. A 380-kb Duplication in 7p22.3 Encompassing the LFNG Gene in a Boy with Asperger Syndrome

    NARCIS (Netherlands)

    Vulto-van Silfhout, A.T.; Brouwer, A.F. de; Leeuw, N. de; Obihara, C.C.; Brunner, H.G.; Vries, B.B. de

    2012-01-01

    De novo genomic aberrations are considered an important cause of autism spectrum disorders. We describe a de novo 380-kb gain in band p22.3 of chromosome 7 in a patient with Asperger syndrome. This duplicated region contains 9 genes including the LNFG gene that is an important regulator of NOTCH

  18. A 380-kb Duplication in 7p22.3 Encompassing the LFNG Gene in a Boy with Asperger Syndrome

    NARCIS (Netherlands)

    Vulto-van Silfhout, A.T.; Brouwer, A.F. de; Leeuw, N. de; Obihara, C.C.; Brunner, H.G.; Vries, B.B. de

    2012-01-01

    De novo genomic aberrations are considered an important cause of autism spectrum disorders. We describe a de novo 380-kb gain in band p22.3 of chromosome 7 in a patient with Asperger syndrome. This duplicated region contains 9 genes including the LNFG gene that is an important regulator of NOTCH sig

  19. A human homologue to the yeast omnipotent suppressor 45 maps 100 kb centromeric to HLA-A

    Energy Technology Data Exchange (ETDEWEB)

    Chauvel, B.; Dorval, I.; Fergelot, P. [CNRS, Faculte de Medecine, Rennes (France)] [and others

    1995-04-01

    Idipathic hemochromatosis is a common autosomal recessive inherited disorder of iron metabolism. The molecular defect is unknown. However, the gene responsible for the disease (HFE) has been localized on the short arm of chromosome 6. It is closely linked to the HLA class I genes and possibly within a 350 kilobase (kb) region around the HLA-A locus. In order to identify candidate genes for hemochromatosis, we applied a cDNA selection technique to isolate transcribed sequences encoded on yeast artificial chromosomes (YAC). At first, we screened a cDNA library derived from normal human duodenal mucosa with the YAC B30 H3. This YAC contains a 320 kb DNA insert including the HLA-A gene and spanning 150 kb of the 350 kb zone where the hemochromatosis gene is in linkage disequilibrium with restriction fragment length polymorphism (RFLP) markers. Preparation of the cDNA library of duodenal mucosa in Lambda Zap II phage and library screening with YAC B30 were carried out as previously described. In this way, we isolated seven non-HLA-A cDNAs corresponding to seven new genomic sequences. These potential genes were named hemochromatosis candidate gene (HCG) and numbered I to VII. The survey of all these cDNAs and their corresponding genomic sequences is in progress. In this work, we are especially interested in one of the seven non-HLA class I cDNA clones, named clone 58. 12 refs., 2 figs.

  20. Geomechanical modeling of surface uplift around well KB-502 at the in Salah CO2 storage site

    NARCIS (Netherlands)

    Fokker, P.A.; Orlic, B.; Meer, L.G.H. van der; Geel, C.R.

    2011-01-01

    Injection of CO2 in the InSalah field has caused uplift of the surface, as observed by satellite geodetic techniques (InSAR). Around one of the wells, KB-502, the uplift shows anomalous behaviour: a two-lobe pattern develops in the direction of the preferred fracture orientation. This indicates the

  1. A 380-kb Duplication in 7p22.3 Encompassing the LFNG Gene in a Boy with Asperger Syndrome

    NARCIS (Netherlands)

    Vulto-van Silfhout, A.T.; Brouwer, A.F. de; Leeuw, N. de; Obihara, C.C.; Brunner, H.G.; Vries, B.B. de

    2012-01-01

    De novo genomic aberrations are considered an important cause of autism spectrum disorders. We describe a de novo 380-kb gain in band p22.3 of chromosome 7 in a patient with Asperger syndrome. This duplicated region contains 9 genes including the LNFG gene that is an important regulator of NOTCH sig

  2. Identification of MHC class I H-2 Kb/Db-restricted immunogenic peptides derived from retinal proteins

    DEFF Research Database (Denmark)

    Wang, Mingjun; Bai, Fang; Pries, Mette

    2006-01-01

    . The immunogenic peptides alone did not induce inflammation in the eyes, but they could enhance severity of uveitis induced by IRBP. CONCLUSIONS: Five of 21 H-2 Kb/Db-binding retinal protein-derived peptides were found to be immunogenic, suggesting that these peptides could function as autoantigenic epitopes...... in the development of inflammatory eye diseases, such as uveitis....

  3. Geomechanical modeling of surface uplift around well KB-502 at the in Salah CO2 storage site

    NARCIS (Netherlands)

    Fokker, P.A.; Orlic, B.; Meer, L.G.H. van der; Geel, C.R.

    2011-01-01

    Injection of CO2 in the InSalah field has caused uplift of the surface, as observed by satellite geodetic techniques (InSAR). Around one of the wells, KB-502, the uplift shows anomalous behaviour: a two-lobe pattern develops in the direction of the preferred fracture orientation. This indicates the

  4. Identification of Cannabis sativa L. using the 1-kbTHCA synthase-fluorescence in situ hybridization probe.

    Science.gov (United States)

    Jeangkhwoa, Pattraporn; Bandhaya, Achirapa; Umpunjun, Puangpaka; Chuenboonngarm, Ngarmnij; Panvisavas, Nathinee

    2017-03-01

    This study reports a successful application of fluorescence in situ hybridization (FISH) technique in the identification of Cannabis sativa L. cells recovered from fresh and dried powdered plant materials. Two biotin-16-dUTP-labeled FISH probes were designed from the Cannabis-specific tetrahydrocannabinolic acid synthase (THCAS) gene and the ITS region of the 45S rRNA gene. Specificity of probe-target hybridization was tested against the target and 4 non-target plant species, i.e., Humulus lupulus, Mitragyna speciosa, Papaver sp., and Nicotiana tabacum. The 1-kb THCA synthase hybridization probe gave Cannabis-specific hybridization signals, unlike the 700-bp Cannabis-ITS hybridization probe. Probe-target hybridization was also confirmed against 20 individual Cannabis plant samples. The 1-kb THCA synthase and 700-bp Cannabis-ITS hybridization probes clearly showed 2 hybridization signals per cell with reproducibility. The 1-kb THCA synthase probe did not give any FISH signal when tested against H. lupulus, its closely related member of the Canabaceae family. It was also showed that 1-kb THCA synthase FISH probe can be applied to identify small amount of dried powdered Cannabis material with an addition of rehydration step prior to the experimental process. This study provided an alternative identification method for Cannabis trace. Copyright © 2016. Published by Elsevier B.V.

  5. The novel Na+/Ca2+ exchange inhibitor KB-R7943 also blocks native and expressed neuronal nicotinic receptors

    Science.gov (United States)

    Pintado, Antonio J; Herrero, Carlos J; García, Antonio G; Montiel, Carmen

    2000-01-01

    We studied the effects of the novel Na+/Ca2+ exchange inhibitor KB-R7943, 2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiourea methanesulphonate, on the native nicotinic receptors present at the bovine adrenal chromaffin cells, as well as on rat brain α3β4 and α7 nicotinic acetylcholine receptors (AChRs) expressed in Xenopus oocytes.As expected, KB-R7943 blocked the Na+-gradient dependent 45Ca2+ uptake into chromaffin cells (IC50 of 5.5 μM); but in addition, the compound also inhibited the 45Ca2+ entry and the increase of cytosolic Ca2+ concentration, [Ca2+]c, stimulated by 5 s pulses of ACh (IC50 of 6.5 and 1.7 μM, respectively).In oocytes expressing α3β4 and α7 nicotinic AChRs, voltage-clamped at −60 mV, inward currents elicited by 1 s pulses of 100 μM ACh (IACh) were blocked by KB-R7943 with an IC50 of 0.4 μM and a Hill coefficient of 0.9.Blockade of α3β4 currents by KB-R7943 was noncompetitive; moreover, the blocker (0.3 μM) became more active as the ACh concentration increased (34 versus 66% blockade at 30 μM and 1 mM ACh, respectively).Inhibition of α3β4 currents by 0.3 μM KB-R7943 was more pronounced at hyperpolarized potentials. If given within the ACh pulse (10 μM), the inhibition amounted to 33, 64 and 80% in oocytes voltage-clamped at −40, −60 and −100 mV, respectively. The onset of blockade was faster and the recovery slower at −100 mV; the reverse was true at −40 mV.In conclusion, KB-R7943 is a potent blocker of nicotinic AChRs; moreover, it displays many features of an open-channel blocker at the rat brain α3β4 AChR. These results should be considered when KB-R7943 is to be used to study Ca2+ homeostasis in cells expressing nicotinic AChRs and the Na+/Ca2+ exchanger. PMID:10952680

  6. Sustaining northern red oak forests: managing oak from regeneration to canopy dominance in mature stands

    Science.gov (United States)

    Daniel C. Dey; Gary W. Miller; John M. Kabrick

    2008-01-01

    Across the range of northern red oak, managers have problems sustaining current stocking of northern red oak in forests. Oak species are adapted to frequent stand disturbances that reduce the abundance of shade tolerant competitors and control fast-growing pioneer species. A widely recommended approach to regenerating northern red oak is to develop relatively large...

  7. FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

    DEFF Research Database (Denmark)

    Simon, Christian; Kudahl, Ulrich Johan; Sun, Jing;

    2015-01-01

    FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity and the analysis of targets of immune...... responses. FluKB consists of more than 400,000 influenza protein sequences, known epitope data (357 verified T-cell epitopes, 685 HLA binders, and 16 naturally processed MHC ligands), and a collection of 28 influenza antibodies and their structurally defined B-cell epitopes. FluKB was built using amodular...

  8. SAFOD Brittle Microstructure and Mechanics Knowledge Base (SAFOD BM2KB)

    Science.gov (United States)

    Babaie, H. A.; Hadizadeh, J.; di Toro, G.; Mair, K.; Kumar, A.

    2008-12-01

    . The web-interface was developed in java using eclipse as the IDE. The web interfaces to query and submit data were implemented applying JSP, servlets, javascript, and AJAX. The Jena API, a Java framework for building Semantic Web applications, was used to develop the web-interface. Jena provided a programmatic environment for RDF, RDFS, OWL, and SPARQL query engine. Building web applications with AJAX helps retrieving data from the server asynchronously in the background without interfering with the display and behavior of the existing page. The application was deployed on an apache tomcat server at GSU. The SAFOD BM2KB website provides user-friendly search, submit, feedback, and other services. The General Search option allows users to search the knowledge base by selecting the classes (e.g., Experiment, Surface Analysis), their respective attributes (e.g., apparatus, date performed), and the relationships to other classes (e.g., Sample, Laboratory). The Search by Sample option allows users to search the knowledge base based on sample number. The Search by Investigator lets users to search the knowledge base by choosing an investigator who is involved in this project. The website also allows users to submit new data. The Submit Data option opens a page where users can submit the SAFOD data to our knowledge base by selecting specific classes and attributes. The submitted data then become available for query as part of the knowledge base. The SAFOD BM2KB can be accessed from the main SAFOD website.

  9. Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome

    Science.gov (United States)

    Lu, Rui; Folimonov, Alexey; Shintaku, Michael; Li, Wan-Xiang; Falk, Bryce W.; Dawson, William O.; Ding, Shou-Wei

    2004-11-01

    Viral infection in both plant and invertebrate hosts requires a virus-encoded function to block the RNA silencing antiviral defense. Here, we report the identification and characterization of three distinct suppressors of RNA silencing encoded by the 20-kb plus-strand RNA genome of citrus tristeza virus (CTV). When introduced by genetic crosses into plants carrying a silencing transgene, both p20 and p23, but not coat protein (CP), restored expression of the transgene. Although none of the CTV proteins prevented DNA methylation of the transgene, export of the silencing signal (capable of mediating intercellular silencing spread) was detected only from the F1 plants expressing p23 and not from the CP- or p20-expressing F1 plants, demonstrating suppression of intercellular silencing by CP and p20 but not by p23. Thus, intracellular and intercellular silencing are each targeted by a CTV protein, whereas the third, p20, inhibits silencing at both levels. Notably, CP suppresses intercellular silencing without interfering with intracellular silencing. The novel property of CP suggests a mechanism distinct to p20 and all of the other viral suppressors known to interfere with intercellular silencing and that this class of viral suppressors may not be consistently identified by Agrobacterium coinfiltration because it also induces RNA silencing against the infiltrated suppressor transgene. Our analyses reveal a sophisticated viral counter-defense strategy that targets the silencing antiviral pathway at multiple steps and may be essential for protecting CTV with such a large RNA genome from antiviral silencing in the perennial tree host. RNA interference | citrus tristeza virus | virus synergy | antiviral immunity

  10. Candidate genes within a 143 kb region of the flower sex locus in Vitis.

    Science.gov (United States)

    Fechter, Iris; Hausmann, Ludger; Daum, Margrit; Sörensen, Thomas Rosleff; Viehöver, Prisca; Weisshaar, Bernd; Töpfer, Reinhard

    2012-03-01

    Wild Vitis species are dioecious plants, while the cultivated counterpart, Vitis vinifera subspec. vinifera, generally shows hermaphroditic flowers. In Vitis the genetic determinants of flower sex have previously been mapped to a region on chromosome 2. In a combined strategy of map-based cloning and the use of the publicly available grapevine reference genome sequence, the structure of the grapevine flower sex locus has been elucidated with the subsequent identification of candidate genes which might be involved in the development of the different flower sex types. In a fine mapping approach, the sex locus in grapevine was narrowed down using a population derived from a cross of a genotype with a Vitis vinifera background ('Schiava Grossa' × 'Riesling') with the male rootstock cv. 'Börner' (V. riparia × V. cinerea). A physical map of 143 kb was established from BAC clones spanning the 0.5 cM region defined by the closest flanking recombination break points. Sequencing and gene annotation of the entire region revealed several candidate genes with a potential impact on flower sex formation. One of the presumed candidate genes, an adenine phosphoribosyltransferase, was analysed in more detail. The results led to the development of a marker for the presence or absence of the female alleles, while the male and hermaphroditic alleles are still to be differentiated. The impact of other candidate genes is discussed, especially with regard to plant hormone actions. The markers developed will permit the selection of female breeding lines which do not require laborious emasculation thus considerably simplifying grapevine breeding. The genetic finger prints displayed that our cultivated grapevines frequently carry a female allele while homozygous hermaphrodites are rare.

  11. Red Bull Crisis

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Additives make Red Bull the first company to test China’s new Food Safety Law "Drink Red Bull when you feel sleepy or tired" is a famous advertising slogan well-known to Chinese. But a recent German

  12. Caught red-handed: Rc encodes a basic helix-loop-helix protein conditioning red pericarp in rice.

    Science.gov (United States)

    Sweeney, Megan T; Thomson, Michael J; Pfeil, Bernard E; McCouch, Susan

    2006-02-01

    Rc is a domestication-related gene required for red pericarp in rice (Oryza sativa). The red grain color is ubiquitous among the wild ancestors of O. sativa, in which it is closely associated with seed shattering and dormancy. Rc encodes a basic helix-loop-helix (bHLH) protein that was fine-mapped to an 18.5-kb region on rice chromosome 7 using a cross between Oryza rufipogon (red pericarp) and O. sativa cv Jefferson (white pericarp). Sequencing of the alleles from both mapping parents as well as from two independent genetic stocks of Rc revealed that the dominant red allele differed from the recessive white allele by a 14-bp deletion within exon 6 that knocked out the bHLH domain of the protein. A premature stop codon was identified in the second mutant stock that had a light red pericarp. RT-PCR experiments confirmed that the Rc gene was expressed in both red- and white-grained rice but that a shortened transcript was present in white varieties. Phylogenetic analysis, supported by comparative mapping in rice and maize (Zea mays), showed that Rc, a positive regulator of proanthocyanidin, is orthologous with INTENSIFIER1, a negative regulator of anthocyanin production in maize, and is not in the same clade as rice bHLH anthocyanin regulators.

  13. Red Wine Over China

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The color red dominates Chinese life and now growing interest in red wine maintains that trend and ushers in a new fashionable addition to local culture There was a time when trying to find a good red wine in China was a difficult affair. But as with all

  14. International Federation of Red Cross and Red Crescent Societies

    Science.gov (United States)

    ... Federation of Red Cross Red Crescent Societies (IFRC)... Hurricane Matthew: International Federation of Red Cross and Red Crescent ... communities Port-au Prince, 3 November 2016 —Since Hurricane Matthew struck Haiti one month ago, the Haiti Re... ...

  15. Mapping autonomously replicating sequence elements in a 73-kb region of chromosome II of the fission yeast, Schizosaccharomyces pombe

    Indian Academy of Sciences (India)

    Vinay Kumar Srivastava; Dharani Dhar Dubey

    2007-08-01

    Autonomously replicating sequence (ARS) elements are the genetic determinants of replication origin function in yeasts. They can be easily identified as the plasmids containing them transform yeast cells at a high frequency. As the first step towards identifying all potential replication origins in a 73-kb region of the long arm of fission yeast chromosome II, we have mapped five new ARS elements using systematic subcloning and transformation assay. 2D analysis of one of the ARS plasmids that showed highest transformation frequency localized the replication origin activity within the cloned genomic DNA. All the new ARS elements are localized in two clusters in centromere proximal 40 kb of the region. The presence of at least six ARS elements, including the previously reported ars727, is suggestive of a higher origin density in this region than that predicted earlier using a computer based search.

  16. Expert curation in UniProtKB: a case study on dealing with conflicting and erroneous data.

    Science.gov (United States)

    Poux, Sylvain; Magrane, Michele; Arighi, Cecilia N; Bridge, Alan; O'Donovan, Claire; Laiho, Kati

    2014-01-01

    UniProtKB/Swiss-Prot provides expert curation with information extracted from literature and curator-evaluated computational analysis. As knowledgebases continue to play an increasingly important role in scientific research, a number of studies have evaluated their accuracy and revealed various errors. While some are curation errors, others are the result of incorrect information published in the scientific literature. By taking the example of sirtuin-5, a complex annotation case, we will describe the curation procedure of UniProtKB/Swiss-Prot and detail how we report conflicting information in the database. We will demonstrate the importance of collaboration between resources to ensure curation consistency and the value of contributions from the user community in helping maintain error-free resources. Database URL: www.uniprot.org.

  17. Fast-earth: A global image caching architecture for fast access to remote-sensing data

    Science.gov (United States)

    Talbot, B. G.; Talbot, L. M.

    We introduce Fast-Earth, a novel server architecture that enables rapid access to remote sensing data. Fast-Earth subdivides a WGS-84 model of the earth into small 400 × 400 meter regions with fixed locations, called plats. The resulting 3,187,932,913 indexed plats are accessed with a rapid look-up algorithm. Whereas many traditional databases store large original images as a series by collection time, requiring long searches and slow access times for user queries, the Fast-Earth architecture enables rapid access. We have prototyped a system in conjunction with a Fast-Responder mobile app to demonstrate and evaluate the concepts. We found that new data could be indexed rapidly in about 10 minutes/terabyte, high-resolution images could be chipped in less than a second, and 250 kB image chips could be delivered over a 3G network in about 3 seconds. The prototype server implemented on a very small computer could handle 100 users, but the concept is scalable. Fast-Earth enables dramatic advances in rapid dissemination of remote sensing data for mobile platforms as well as desktop enterprises.

  18. Low-temperature change of lattice parameters in LiKB{sub 4}O{sub 7} single crystals

    Energy Technology Data Exchange (ETDEWEB)

    Adamiv, Volodymyr; Burak, Yaroslav; Teslyuk, Ihor [Institute of Physical Optics, Lviv (Ukraine)

    2009-02-15

    Structural investigations of LiKB{sub 4}O{sub 7} crystals in the 13-290 K temperature range have been carried out by means of the high-resolution powder diffraction technique applying synchrotron radiation. It is shown that the structural phase transitions are absent and all the linear thermal expansion coefficients {alpha}{sub ij} are positive in the investigated temperature range. (copyright 2009 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  19. A 32 kb critical region excluding Y402H in CFH mediates risk for age-related macular degeneration.

    Science.gov (United States)

    Sivakumaran, Theru A; Igo, Robert P; Kidd, Jeffrey M; Itsara, Andy; Kopplin, Laura J; Chen, Wei; Hagstrom, Stephanie A; Peachey, Neal S; Francis, Peter J; Klein, Michael L; Chew, Emily Y; Ramprasad, Vedam L; Tay, Wan-Ting; Mitchell, Paul; Seielstad, Mark; Stambolian, Dwight E; Edwards, Albert O; Lee, Kristine E; Leontiev, Dmitry V; Jun, Gyungah; Wang, Yang; Tian, Liping; Qiu, Feiyou; Henning, Alice K; LaFramboise, Thomas; Sen, Parveen; Aarthi, Manoharan; George, Ronnie; Raman, Rajiv; Das, Manmath Kumar; Vijaya, Lingam; Kumaramanickavel, Govindasamy; Wong, Tien Y; Swaroop, Anand; Abecasis, Goncalo R; Klein, Ronald; Klein, Barbara E K; Nickerson, Deborah A; Eichler, Evan E; Iyengar, Sudha K

    2011-01-01

    Complement factor H shows very strong association with Age-related Macular Degeneration (AMD), and recent data suggest that multiple causal variants are associated with disease. To refine the location of the disease associated variants, we characterized in detail the structural variation at CFH and its paralogs, including two copy number polymorphisms (CNP), CNP147 and CNP148, and several rare deletions and duplications. Examination of 34 AMD-enriched extended families (N = 293) and AMD cases (White N = 4210 Indian = 134; Malay = 140) and controls (White N = 3229; Indian = 117; Malay = 2390) demonstrated that deletion CNP148 was protective against AMD, independent of SNPs at CFH. Regression analysis of seven common haplotypes showed three haplotypes, H1, H6 and H7, as conferring risk for AMD development. Being the most common haplotype H1 confers the greatest risk by increasing the odds of AMD by 2.75-fold (95% CI = [2.51, 3.01]; p = 8.31×10(-109)); Caucasian (H6) and Indian-specific (H7) recombinant haplotypes increase the odds of AMD by 1.85-fold (p = 3.52×10(-9)) and by 15.57-fold (P = 0.007), respectively. We identified a 32-kb region downstream of Y402H (rs1061170), shared by all three risk haplotypes, suggesting that this region may be critical for AMD development. Further analysis showed that two SNPs within the 32 kb block, rs1329428 and rs203687, optimally explain disease association. rs1329428 resides in 20 kb unique sequence block, but rs203687 resides in a 12 kb block that is 89% similar to a noncoding region contained in ΔCNP148. We conclude that causal variation in this region potentially encompasses both regulatory effects at single markers and copy number.

  20. A 32 kb critical region excluding Y402H in CFH mediates risk for age-related macular degeneration.

    Directory of Open Access Journals (Sweden)

    Theru A Sivakumaran

    Full Text Available Complement factor H shows very strong association with Age-related Macular Degeneration (AMD, and recent data suggest that multiple causal variants are associated with disease. To refine the location of the disease associated variants, we characterized in detail the structural variation at CFH and its paralogs, including two copy number polymorphisms (CNP, CNP147 and CNP148, and several rare deletions and duplications. Examination of 34 AMD-enriched extended families (N = 293 and AMD cases (White N = 4210 Indian = 134; Malay = 140 and controls (White N = 3229; Indian = 117; Malay = 2390 demonstrated that deletion CNP148 was protective against AMD, independent of SNPs at CFH. Regression analysis of seven common haplotypes showed three haplotypes, H1, H6 and H7, as conferring risk for AMD development. Being the most common haplotype H1 confers the greatest risk by increasing the odds of AMD by 2.75-fold (95% CI = [2.51, 3.01]; p = 8.31×10(-109; Caucasian (H6 and Indian-specific (H7 recombinant haplotypes increase the odds of AMD by 1.85-fold (p = 3.52×10(-9 and by 15.57-fold (P = 0.007, respectively. We identified a 32-kb region downstream of Y402H (rs1061170, shared by all three risk haplotypes, suggesting that this region may be critical for AMD development. Further analysis showed that two SNPs within the 32 kb block, rs1329428 and rs203687, optimally explain disease association. rs1329428 resides in 20 kb unique sequence block, but rs203687 resides in a 12 kb block that is 89% similar to a noncoding region contained in ΔCNP148. We conclude that causal variation in this region potentially encompasses both regulatory effects at single markers and copy number.

  1. Expression of nuclear factor-KB in hepatocellular carcinoma and its relation with .the X protein of hepatitis B virus

    Institute of Scientific and Technical Information of China (English)

    Shuang Ping Guo; Wen Liang Wang; Yu Qiang Zhai; Yi Ling Zhao

    2001-01-01

    AIM In this study we investigated therelationship of the X protein of HBV and nuclearfactor-KB (NF-κB) and the expression of NF-KB inhuman hepatocellular carcinoma tissues.METHODS Immunohistochemistry SP methodwas used to detect the expression of NF-κB and the X protein of HBV in human hepatocellularcarcinoma tissues of 52 cases. Gene transfectionmediated by lipofectamine was used to transfectthe eukaryotic expression vector pCDNA3. 1-HBXof HBV x gene into human hepatocellularcarcinoma cell line HCC-9204 and NF-κB wasdetected.RESULTS NF-κB was widely expressed inhuman hepatocellular carcinoma tissues in atotal of 52 cases and its expression was relatedto the X protein of HBV. NF-KB was localizedboth in the cytoplasm and . The nuclei ofhepatocellular carcinoma cells in 11 cases whichwere positive for the X protein of HBV while in 41cases negative for the X protein of HBV, NF-Kbwas only localized in the cytoplasm ofhepatocellular carcinoma cells but translocatedto the nuclei of hepatocellular carcinoma cellsafter the eukaryotic expression vectorpCDNA3.1-HBX was transfected into HCC-9204cells.CONCLUSION This study strongly suggeststhat the nuclear factor NF-KB is widely expressedin hepatocellular carcinoma tissues in differentstyles according to the expression of the Xprotein of HBV. NF-κB is abnormally activated inhepatocellular carcinoma, which is probablyrelated to the X protein of HBV. The X protein ofHBV can activate NF-κB to translocate into nucleiof hepatocellular carcinoma cells.

  2. Amino and nitro derivatives of 5,7-dimethoxyflavone from Kaempferia parviflora and cytotoxicity against KB cell line.

    Science.gov (United States)

    Wanich, Suchana; Yenjai, Chavi

    2009-09-01

    Structural modification of 5,7-dimethoxyflavone isolated from Kaempferia parviflora furnished two nitro and seven amino derivatives. Among these, six new (3, 5-6, 8-10) and three known (2, 4, 7) flavonoid derivatives were synthesized. All compounds were evaluated for cytotoxicity against KB cell line using colorimetric method. Compounds 6 and 8 exhibited strong cytotoxicity with IC50 values of 6.80 and 5.84 microg/mL, respectively.

  3. FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

    DEFF Research Database (Denmark)

    Simon, Christian; Kudahl, Ulrich Johan; Sun, Jing;

    2015-01-01

    FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity and the analysis of targets of immune...... responses. FluKB consists of more than 400,000 influenza protein sequences, known epitope data (357 verified T-cell epitopes, 685 HLA binders, and 16 naturally processed MHC ligands), and a collection of 28 influenza antibodies and their structurally defined B-cell epitopes. FluKB was built using amodular......KB is representation of a new generation of databases that integrates data, analytical tools, and analytical workflows that enable comprehensive analysis and automatic generation of analysis reports....

  4. FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses

    DEFF Research Database (Denmark)

    Simon, Christian; Kudahl, Ulrich Johan; Sun, Jing

    2015-01-01

    FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity and the analysis of targets of immune......-cell vaccine targets and assessment of neutralizing antibody coverage. FluKB supports the discovery of vaccine targets and the analysis of viral diversity and its implications for vaccine discovery as well as potential T-cell breadth and antibody cross neutralization involving multiple strains. Flu...... responses. FluKB consists of more than 400,000 influenza protein sequences, known epitope data (357 verified T-cell epitopes, 685 HLA binders, and 16 naturally processed MHC ligands), and a collection of 28 influenza antibodies and their structurally defined B-cell epitopes. FluKB was built using amodular...

  5. FAST: FAST Analysis of Sequences Toolbox.

    Science.gov (United States)

    Lawrence, Travis J; Kauffman, Kyle T; Amrine, Katherine C H; Carper, Dana L; Lee, Raymond S; Becich, Peter J; Canales, Claudia J; Ardell, David H

    2015-01-01

    FAST (FAST Analysis of Sequences Toolbox) provides simple, powerful open source command-line tools to filter, transform, annotate and analyze biological sequence data. Modeled after the GNU (GNU's Not Unix) Textutils such as grep, cut, and tr, FAST tools such as fasgrep, fascut, and fastr make it easy to rapidly prototype expressive bioinformatic workflows in a compact and generic command vocabulary. Compact combinatorial encoding of data workflows with FAST commands can simplify the documentation and reproducibility of bioinformatic protocols, supporting better transparency in biological data science. Interface self-consistency and conformity with conventions of GNU, Matlab, Perl, BioPerl, R, and GenBank help make FAST easy and rewarding to learn. FAST automates numerical, taxonomic, and text-based sorting, selection and transformation of sequence records and alignment sites based on content, index ranges, descriptive tags, annotated features, and in-line calculated analytics, including composition and codon usage. Automated content- and feature-based extraction of sites and support for molecular population genetic statistics make FAST useful for molecular evolutionary analysis. FAST is portable, easy to install and secure thanks to the relative maturity of its Perl and BioPerl foundations, with stable releases posted to CPAN. Development as well as a publicly accessible Cookbook and Wiki are available on the FAST GitHub repository at https://github.com/tlawrence3/FAST. The default data exchange format in FAST is Multi-FastA (specifically, a restriction of BioPerl FastA format). Sanger and Illumina 1.8+ FastQ formatted files are also supported. FAST makes it easier for non-programmer biologists to interactively investigate and control biological data at the speed of thought.

  6. FAST: FAST Analysis of Sequences Toolbox

    Directory of Open Access Journals (Sweden)

    Travis J. Lawrence

    2015-05-01

    Full Text Available FAST (FAST Analysis of Sequences Toolbox provides simple, powerful open source command-line tools to filter, transform, annotate and analyze biological sequence data. Modeled after the GNU (GNU’s Not Unix Textutils such as grep, cut, and tr, FAST tools such as fasgrep, fascut, and fastr make it easy to rapidly prototype expressive bioinformatic workflows in a compact and generic command vocabulary. Compact combinatorial encoding of data workflows with FAST commands can simplify the documentation and reproducibility of bioinformatic protocols, supporting better transparency in biological data science. Interface self-consistency and conformity with conventions of GNU, Matlab, Perl, BioPerl, R and GenBank help make FAST easy and rewarding to learn. FAST automates numerical, taxonomic, and text-based sorting, selection and transformation of sequence records and alignment sites based on content, index ranges, descriptive tags, annotated features, and in-line calculated analytics, including composition and codon usage. Automated content- and feature-based extraction of sites and support for molecular population genetic statistics makes FAST useful for molecular evolutionary analysis. FAST is portable, easy to install and secure thanks to the relative maturity of its Perl and BioPerl foundations, with stable releases posted to CPAN. Development as well as a publicly accessible Cookbook and Wiki are available on the FAST GitHub repository at https://github.com/tlawrence3/FAST. The default data exchange format in FAST is Multi-FastA (specifically, a restriction of BioPerl FastA format. Sanger and Illumina 1.8+ FastQ formatted files are also supported. FAST makes it easier for non-programmer biologists to interactively investigate and control biological data at the speed of thought.

  7. Culture conditions for yellow pigment formation byMonascus sp. KB 10 grown on cassava medium.

    Science.gov (United States)

    Yongsmith, B; Tabloka, W; Yongmanitchai, W; Bavavoda, R

    1993-01-01

    An isolate ofMonascus, from a commercial, fermented soybean curd (sufu) was grown on a cassava medium. With medium at an initial pH of 7.0 an orange-red pigmentation was produced but with an initial pH below 4, a light golden pigment was obtained. A medium containing, w/v, 3% cassava starch, 0.4% peptone and 0.1% glutamic acid, with an initial pH of 2.5 was optimal for the production of this yellow pigment, which had a single maximum absorption spectrum at 330 nm. The spectroscopic characterization of the purified yellow pigments demonstrated a monascin-ankaflavin-monascorubrin skeleton.

  8. Linkage disequilibrium mapping identifies a 390 kb region associated with CYP2D6 poor drug metabolising activity.

    Science.gov (United States)

    Hosking, L K; Boyd, P R; Xu, C F; Nissum, M; Cantone, K; Purvis, I J; Khakhar, R; Barnes, M R; Liberwirth, U; Hagen-Mann, K; Ehm, M G; Riley, J H

    2002-01-01

    The cytochrome p450 enzyme, CYP2D6, metabolises approximately 20% of marketed drugs. CYP2D6 multiple variants are associated with altered enzyme activities. Genotyping 1018 Caucasians for CYP2D6 polymorphisms (G1846A, delT1707, delA2549 and A2935C), known to result in the recessive CYP2D6 poor drug metaboliser (PM) phenotype, identified 41 individuals with predicted PM phenotype. These 41 individuals were classified as 'cases'. Single nucleotide polymorphisms (SNPs) mapping within an 880 kb region flanking CYP2D6, were identified to evaluate potential association between genetic variation and the CYP2D6 PM phenotype. The 41 PM cases and 977 controls were genotyped and analysed for 27 SNPs. Associations were observed across a 390 kb region between 14 SNPs and the PM phenotype (P values from 6.20 x 10(-4) to 4.54 x 10(-35)). Haplotype analysis revealed more significant levels of association (P = 3.54 x 10(-56)). Strong (D' > 0.7) linkage disequilibrium (LD) between SNPs was observed across the same 390 kb region associated with the CYP2D6 phenotype. The observed phenotype:genotype association reached genome-wide levels of significance, and supports the strategy for potential application of LD mapping and whole genome association scans to pharmacogenetic studies.

  9. Comparative Analysis of the 100kb Region Containing the Pi-kh Locus Between indica and japonica Rice Lines

    Institute of Scientific and Technical Information of China (English)

    S.P. Kumar; V. Dalal; N.K. Singh; T.R. Sharma

    2007-01-01

    We have recently cloned a pathogen inducible blast resistance gene Pi-kh from the indica rice line Tetep using a positional cloning approach. In this study, we carried out structural organization analysis of the Pi-kh locus in both indica and japonica rice lines. A 100 kb region containing 50 kb upstream and 50 kb downstream sequences flanking to the Pi-kh locus was selected for the investigation. A total of 16 genes in indica and 15 genes in japonica were predicted and annotated in this region. The average GC content of indica and japonica genes in this region was 53.15% and 49.3%, respectively. Both indica and japonica sequences were polymorphic for simple sequence repeats having mono-, di-, tri-, tetra-, and pentanucleotides. Sequence analysis of the specific blast resistant Pi-kh allele of Tetep and the susceptible Pi-kh allele of the japonica rice line Nipponbare showed differences in the number and distribution of motifs involved in phosphorylation, resulting in the resistance phenotype in Tetep.

  10. Anonymous marker loci within 400 kb of HLA-A generate haplotypes in linkage disequilibrium with the hemochromatosis gene (HFE)

    Energy Technology Data Exchange (ETDEWEB)

    Yaouanq, J.; Perichon, M.; Treut, A.L.; Kahloun, A.E.; Mauvieux, V.; Blayau, M.; Jouanolle, A.M.; Chauvel, B.; Le Gall, J.Y.; David, V. (Faculte de Medicine, Rennes (France)) (and others)

    1994-02-01

    The hemochromatosis gene (HFE) maps to 6p21.3 and is less than 1 cM from the HLA class I gene; however, the precise physical location of the gene has remained elusive and controversial. The unambiguous identification of a crossover event within hemochromatosis families is very difficult; it is particularly hampered by the variability of the phenotypic expression as well as by the sex- and age-related penetrance of the disease. For these considerations, traditional linkage analysis could prove of limited value in further refining the extrapolated physical position of HFE. The authors therefore embarked upon a linkage-disequilibrium analysis of HFE and normal chromosomes for the Brittany population. In this report, 66 hemochromatosis families yielding 151 hemochromatosis chromosomes and 182 normal chromosomes were RFLP-typed with a battery of probes, including two newly derived polymorphic markers from the 6.7 and HLA-F loci located 150 and 250 kb telomeric to HLA-A, respectively. The results suggest a strong peak of existing linkage disequilibrium focused within the i82-to-6.7 interval (approximately 250 kb). The zone of linkage disequilibrium is flanked by the i97 locus, positioned 30 kb proximal to i82, and the HLA-F gene, found 250 kb distal to HLA-A, markers of which display no significant association with HFE. These data support the possibility that HFE resides within the 400-kb expanse of DNA between i97 and HLA-F. Alternatively, the very tight association of HLA-A3 and allele 1 of the 6.7 locus, both of which are comprised by the major ancestral or founder HFE haplotype in Brittany, supports the possibility that the disease gene may reside immediately telomeric to the 6.7 locus within the linkage-disequilibrium zone. Additionally, hemochromatosis haplotypes possessing HLA-A11 and the low-frequency HLA-F polymorphism (allele 2) are supportive of a separate founder chromosome containing a second, independently arising mutant allele. 69 refs., 1 fig., 5 tabs.

  11. Enrichment of Fetal Nucleated Red Blood Cell in Peripheral Blood of Pregnant Woman by Joint Use of Density Gradient Centrifugation and Keihuaer Acid-fast Staining%密度梯度离心结合Keihuaer抗酸染色法富集孕妇外周血中FNRBC的研究

    Institute of Scientific and Technical Information of China (English)

    何国平; 刘雨生; 童先宏; 郑圣霞; 张荣; 王念念; 吴丽敏; 周桂香

    2009-01-01

    Objective To investigate and establish a simple, rapid and lower-cost method to enrich fetal nucleated red blood cell(FNRBC) in peripheral blood of pregnant woman. Methods About 10 ml of peripheral blood sample from 18 pregnant women with gestational weeks arranged from 8 to 16 was collected, and then subjected to the discontinuous density gradient centrifugation. After the primary cell isolation, Keihuaer acid-fast staining method was used to mark the isolated cells previously spread on the slides. The special morphocytology and colour staining of FNRBC could be distinctly distinguished from the surrounding mother cells. Following that, the special antibody against fetal hemoglobin was further used to validate the fetus-originated characteristics for the positive FNRBC staining slides.Results After dealing with Keihuaer acid-fast staining, the cytoplasm of positive FNRBC was stained to be scarlet,nucleolus to be blue,while the cytoplasm of mother cells were colorless. The positive FNRBC were detected in 14 of 18 healthy pregnant women, average 1 to 6 cells per case,the positive rate was 77.8%. The same experimental results was further validated with the special antibody against fetal hemoglobin immunocytochemical staining,the diagnose accordance rate was 100.0%. Conclusions The density gradient centrifugation,together with Keihuaer acid-fast staining were highly special for the enrichment of FNRBC in peripheral blood of pregnant woman. The practical operation of this method was simple,fast and the future investigations would hopefully prove its worth in the clinical application of noninvasive prenatal diagnosis of gene disorders.%目的 探索建立一种简便、快速、较低成本的用于孕妇外周血胎儿有核红细胞(FNRBC)富集的实验方法.方法 采集18名孕周在8~16周的孕妇外周血10 ml,经Percoll不连续密度梯度离心初步分离后,运用Keihuaer抗酸染色法对玻片上的细胞进行染色标记,显微镜下观察、辨

  12. Ruijin Women In the Period of the Red Army (Ⅱ)

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    IN the 1930s, the first red political power was set up in southern China. Women in the area cut their hair short and unbound their feet. But its significance was more than that—women had broken the feudal yokes restraining their hearts. They were able to get married by their own will and stand arm-in-arm beside men. To allow the Red Army to move fast and fight against the

  13. A Leaf-Inhabiting Endophytic Bacterium, Rhodococcus sp. KB6, Enhances Sweet Potato Resistance to Black Rot Disease Caused by Ceratocystis fimbriata.

    Science.gov (United States)

    Hong, Chi Eun; Jeong, Haeyoung; Jo, Sung Hee; Jeong, Jae Cheol; Kwon, Suk Yoon; An, Donghwan; Park, Jeong Mee

    2016-03-01

    Rhodococcus species have become increasingly important owing to their ability to degrade a wide range of toxic chemicals and produce bioactive compounds. Here, we report isolation of the Rhodococcus sp. KB6, which is a new leaf-inhabiting endophytic bacterium that suppresses black rot disease in sweet potato leaves. We determined the 7.0 Mb draft genome sequence of KB6 and have predicted 19 biosynthetic gene clusters for secondary metabolites, including heterobactins, which are a new class of siderophores. Notably, we showed the first internal colonization of host plants with Rhodococcus sp. KB6 and discuss its potential as a biocontrol agent for sustainable agriculture.

  14. Inquiring into Red/Red Inquiring

    Directory of Open Access Journals (Sweden)

    Ken Gale

    2013-05-01

    Full Text Available This layered account of an inquiry into ‘red’ emerged out of a collective biography workshop. In the middle of the Wiltshire countryside, an international and interdisciplinary group of scholars gathered together to write and make other things and marks on paper that asked questions of, and into, the spaces between words, people, things and their environments. We did not set out to workshop or write into or paint ‘red’ but, rather, it was red that slipped in, uninvited, and painted and wrote us. Red arose as a blush or a stain seeping amongst us that became referenced obliquely by material objects, metaphors and fairytales. The stain spread, became noticeable through our weekend together and beyond it, creating another (bright red artery vein of connection to write with.

  15. X-ray and thermoluminescence of LiKB{sub 4}O{sub 7} single crystals

    Energy Technology Data Exchange (ETDEWEB)

    Adamiv, V.T. [Institute of Physical Optics, 23 Dragomanov Str., 79005 Lviv (Ukraine)], E-mail: adamiv@ifo.lviv.ua; Antonyak, O.T. [Physical Department, I. Franko Lviv State University, 8 Kyryla and Mefodiya Street, 79005 Lviv (Ukraine); Burak, Ya.V.; Teslyuk, I.M. [Institute of Physical Optics, 23 Dragomanov Str., 79005 Lviv (Ukraine)

    2008-03-15

    The thermoluminescent (TL) and X-ray luminescent (XL) spectra of undoped LiKB{sub 4}O{sub 7} (LKBO) single crystals had been investigated in the temperature range 80-300 K. It was found that in LKBO crystals, there are two intensive TL peaks at 112 and 132 K. The only one band emission spectra of sharply defined Gaussian shape, confirming the same mechanism of XL and TL by the radiation annihilation of the strongly localized self-trapped excitons (STE), had been observed in the TL and XL spectra. The possible models of these localization centers STE have been discussed.

  16. Calcium exchange between olivine and clinopyroxene calibrated as a geothermobarometer for natural peridotites from 2 to 60 kb with applications

    Science.gov (United States)

    Koehler, T. P.; Brey, G. P.

    1990-09-01

    The equilibrium Ca content of olivine coexisting with clinopyroxene was determined (using an electron microprobe) in natural lherzolitic compositions Mg/(Mg + Fe)ol of about 0.9, in experiments covering lherzolitic systems from 2-60 kb and 900-1400 C. The boundary fluorescence effect was avoided by collecting analytical data from separated mineral grains. The experimental data on Ca solubility were fitted to two equations given in the form of barometers. It was found that calculated P,T conditions for spinel peridotite nodules from southwestern USA, fell within the spinel stablity field and along predicted geotherms for this region.

  17. Novel 31.2 kb α0 Deletion in a Palestinian Family with α-Thalassemia

    DEFF Research Database (Denmark)

    Brieghel, Christian; Birgens, Henrik; Frederiksen, Henrik

    2015-01-01

    A previously unknown α(0) deletion, designated - -(DANE), was found in three generations of a Danish family of Palestinian origin. Six patients were heterozygous and three patients had deletional Hb H (β4) disease with a compound heterozygosity for the common -α(3.7) (rightward) deletion. Multiplex...... ligation-dependent probe amplification (MLPA) supplemented by repeated polymerase chain reaction (PCR) amplification identified the 5' and 3' breakpoints in the α-globin gene cluster. This novel 31.2 kb deletion (NG_000006.1: g.8800_40007del31208) leads to the removal of the HBZ, HBA2 and HBA1 genes....

  18. Severe fetal and neonatal hemolytic anemia due to a 198 kb deletion removing the complete β-globin gene cluster.

    Science.gov (United States)

    Verhovsek, Madeleine; Shah, Nirmish R; Wilcox, Ibifiri; Koenig, Sara C; Barros, Tiago; Thornburg, Courtney D; Steinberg, Martin H; Luo, Hong-yuan; Chui, David H K

    2012-11-01

    Fetal and neonatal hemolytic anemia can be caused by (γδβ)(0)-thalassemia deletions of the β-globin gene cluster. Many of these deletions have not been well characterized, and diagnostic tests are not readily available, thus hampering carrier detection, family counseling, and antenatal diagnosis. We report and define a 198 kb deletion removing the entire β-globin gene cluster, which was found in members of a multigeneration family of Irish/Scottish descent. The proband had life-threatening fetal and neonatal hemolytic anemia which subsided by 1 year of age. Copyright © 2012 Wiley Periodicals, Inc.

  19. 高能大恒KB1800直线加速器MOD连锁故障维修%Introduction of Maintenance of high energy DAHENG KB1800 linear accelerator MOD Chain

    Institute of Scientific and Technical Information of China (English)

    何自怀; 戴国胜; 刘丰华

    2013-01-01

    简要叙述高能大恒KB1800型医用电子直线加速器MOD连锁故障,掌握MOD故障分析和排除的方法,并通过分析速调管故障和处理过程总结此类故障的处理思路、方法和经验.速调管是电子直线加速器中相当昂贵和重要的部件,在安装与拆卸速调管时必须避免水进入高压油箱,否则将导致所有高压油报废,工作人员要严格遵守操作规程并按照维护与保养要求进行维修.%This paper briefly describes the MOD cascading failures of the high energy DAHENG KB1800 medical linear accelerator, mastered the MOD failure by analysis and troubleshooting, and summarizes the relevant solutions and experience by analyzing the failures. The klystron electron linear accelerator is one of important parts and quite expensive. Klystron installation and removal should be guaranteed to prevent water entering the high-pressure fuel tank, otherwise it will lead all the high-pressure oil scrapped. The workers must abide the rules and perform maintenance strictly.

  20. The Red Mansion Banquet

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The concept of the Red Mansion Banquet came from the famous Qing Dynasty classic novel, A Dream of Red Mansions. Each of its courses has, based on descriptions from the book, been carefully planned by the Grand View Garden Hotel chefs. Diners’ senses of sight, sound, and taste are thus regaled with the finest in food, wine and music.

  1. Red Sandalwood Treasure

    Institute of Scientific and Technical Information of China (English)

    INESAPLESKACHEUSKAYA

    2003-01-01

    HAVE you ever come across red sandalwood, a timber so rare as to render it precious? In ancient Asia it was regarded as equal to old and jade. Only the Emperor of China and members of his family had furniture made from red sandalwood, and 10-inch long panels of this material decorated Napoleon's coffin.

  2. Draft Genome Sequence of the Endophytic Strain Rhodococcus kyotonensis KB10, a Potential Biodegrading and Antibacterial Bacterium Isolated from Arabidopsis thaliana

    Science.gov (United States)

    Hong, Chi Eun; Jo, Sung Hee

    2016-01-01

    Rhodococcus kyotonensis KB10 is an endophytic bacterium isolated from Arabidopsis thaliana. The organism showed mild antibacterial activity against the phytopathogen Pseudomonas syringae pv. tomato DC3000. This study reports the genome sequence of R. kyotonensis KB10. This bacterium contains an ectoine biosynthesis gene cluster and has the potential to degrade nitroaromatic compounds. The identified bacterium may be a suitable biocontrol agent and degrader of environmental pollutants. PMID:27389269

  3. Genetic variations and diseases in UniProtKB/Swiss-Prot: the ins and outs of expert manual curation.

    Science.gov (United States)

    Famiglietti, Maria Livia; Estreicher, Anne; Gos, Arnaud; Bolleman, Jerven; Géhant, Sébastien; Breuza, Lionel; Bridge, Alan; Poux, Sylvain; Redaschi, Nicole; Bougueleret, Lydie; Xenarios, Ioannis

    2014-08-01

    During the last few years, next-generation sequencing (NGS) technologies have accelerated the detection of genetic variants resulting in the rapid discovery of new disease-associated genes. However, the wealth of variation data made available by NGS alone is not sufficient to understand the mechanisms underlying disease pathogenesis and manifestation. Multidisciplinary approaches combining sequence and clinical data with prior biological knowledge are needed to unravel the role of genetic variants in human health and disease. In this context, it is crucial that these data are linked, organized, and made readily available through reliable online resources. The Swiss-Prot section of the Universal Protein Knowledgebase (UniProtKB/Swiss-Prot) provides the scientific community with a collection of information on protein functions, interactions, biological pathways, as well as human genetic diseases and variants, all manually reviewed by experts. In this article, we present an overview of the information content of UniProtKB/Swiss-Prot to show how this knowledgebase can support researchers in the elucidation of the mechanisms leading from a molecular defect to a disease phenotype.

  4. The mitochondrial genome and a 60-kb nuclear DNA segment from Naegleria fowleri, the causative agent of primary amoebic meningoencephalitis.

    Science.gov (United States)

    Herman, Emily K; Greninger, Alexander L; Visvesvara, Govinda S; Marciano-Cabral, Francine; Dacks, Joel B; Chiu, Charles Y

    2013-01-01

    Naegleria fowleri is a unicellular eukaryote causing primary amoebic meningoencephalitis, a neuropathic disease killing 99% of those infected, usually within 7-14 days. Naegleria fowleri is found globally in regions including the US and Australia. The genome of the related nonpathogenic species Naegleria gruberi has been sequenced, but the genetic basis for N. fowleri pathogenicity is unclear. To generate such insight, we sequenced and assembled the mitochondrial genome and a 60-kb segment of nuclear genome from N. fowleri. The mitochondrial genome is highly similar to its counterpart in N. gruberi in gene complement and organization, while distinct lack of synteny is observed for the nuclear segments. Even in this short (60-kb) segment, we identified examples of potential factors for pathogenesis, including ten novel N. fowleri-specific genes. We also identified a homolog of cathepsin B; proteases proposed to be involved in the pathogenesis of diverse eukaryotic pathogens, including N. fowleri. Finally, we demonstrate a likely case of horizontal gene transfer between N. fowleri and two unrelated amoebae, one of which causes granulomatous amoebic encephalitis. This initial look into the N. fowleri nuclear genome has revealed several examples of potential pathogenesis factors, improving our understanding of a neglected pathogen of increasing global importance.

  5. Synthesis, characterization and in vitro anti-cancer evaluation of hesperetin-loaded nanoparticles in human oral carcinoma (KB) cells

    Science.gov (United States)

    Gurushankar, K.; Gohulkumar, M.; Rajendra Prasad, N.; Krishnakumar, N.

    2014-03-01

    Hesperetin (HET), a naturally occurring plant bioflavonoid present in citrus fruits, possesses potential anti-inflammatory and anti-carcinogenic activities but poor aqueous solubility limits its applications. To improve its applicability in cancer therapy, hesperetin was encapsulated in Eudragit® E (EE) 100 nanoparticles in the presence of polyvinyl alcohol (PVA) as a stabilizer and its anticancer efficacy in oral carcinoma (KB) cells was studied. Hesperetin-loaded nanoparticles (HETNPs) were prepared by nanoprecipitation method and characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), and x-ray diffraction (XRD). The results thus displayed that the prepared nanoparticles showed a particle size in the range from 55 to 180 nm. The encapsulation efficiency of hesperetin was 83.4% obtained by UV spectroscopy. The in vitro release kinetics of hesperetin under physiological condition show initial rapid release followed by slow and sustained release. 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay revealed higher cytotoxic efficacy of HETNPs than native hesperetin in KB cells. Further, it has been found that reactive oxygen species (ROS) generation, DNA damage and apoptotic indices in HETNPs treated cells are greater than those in native hesperetin treatment. Hence these findings demonstrate that HETNPs could be a potentially useful drug delivery system to produce better hesperetin therapeutics of cancers.

  6. FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses.

    Science.gov (United States)

    Simon, Christian; Kudahl, Ulrich J; Sun, Jing; Olsen, Lars Rønn; Zhang, Guang Lan; Reinherz, Ellis L; Brusic, Vladimir

    2015-01-01

    FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis of influenza sequence diversity and the analysis of targets of immune responses. FluKB consists of more than 400,000 influenza protein sequences, known epitope data (357 verified T-cell epitopes, 685 HLA binders, and 16 naturally processed MHC ligands), and a collection of 28 influenza antibodies and their structurally defined B-cell epitopes. FluKB was built using a modular framework allowing the implementation of analytical workflows and includes standard search tools, such as keyword search and sequence similarity queries, as well as advanced tools for the analysis of sequence variability. The advanced analytical tools for vaccine discovery include visual mapping of T- and B-cell vaccine targets and assessment of neutralizing antibody coverage. FluKB supports the discovery of vaccine targets and the analysis of viral diversity and its implications for vaccine discovery as well as potential T-cell breadth and antibody cross neutralization involving multiple strains. FluKB is representation of a new generation of databases that integrates data, analytical tools, and analytical workflows that enable comprehensive analysis and automatic generation of analysis reports.

  7. Alpinia pricei Rhizome Extracts Induce Cell Cycle Arrest in Human Squamous Carcinoma KB Cells and Suppress Tumor Growth in Nude Mice

    Directory of Open Access Journals (Sweden)

    You-Cheng Hseu

    2011-01-01

    Full Text Available Alpinia pricei has been shown to induce apoptosis in human squamous carcinoma (KB cells. In this study, we report the effectiveness of the ethanol (70% extracts of A. pricei rhizome (AP extracts in terms of tumor regression as determined using both in vitro cell culture and in vivo athymic nude mice models of KB cells. We found that the AP extract (25–200 μg/mL treatment decreased the proliferation of KB cells by arresting progression through the G2/M phase of the cell cycle. This cell cycle blockade was associated with reductions in cyclin A and B1, Cdc2, and Cdc25C, and increased p21/WAF1, Wee1, p53 and phospho-p53 (p-p53 in a dose- and time-dependent manner. Moreover, we found that AP extract treatment decreased metalloproteinase-9 (MMP-9 and urokinase plasminogen activator (u-PA expression, while expression of their endogenous inhibitors, tissue inhibitor of MMP-1 (TIMP-1 and plasminogen activator inhibitor-1 (PAI-1, were increased in KB cells. Furthermore, AP extract treatment effectively delayed tumor incidence in nude mice inoculated with KB cells and reduced the tumor burden. AP extract treatment also induced apoptotic DNA fragmentation, as detected by in situ TUNEL staining. Thus, A. pricei may possess antitumor activity in human squamous carcinoma (KB cells.

  8. Effects of insulin on ketogenesis following fasting in lean and obese men.

    Science.gov (United States)

    Soeters, Maarten R; Sauerwein, Hans P; Faas, Linda; Smeenge, Martijn; Duran, Marinus; Wanders, Ronald J; Ruiter, An F; Ackermans, Mariëtte T; Fliers, Eric; Houten, Sander M; Serlie, Mireille J

    2009-07-01

    The ketone bodies (KBs) D-3-hydroxybutyrate (D-3HB) and acetoacetate (AcAc) play a role in starvation and have been associated with insulin resistance. The dose-response relationship between insulin and KBs was demonstrated to be shifted to the right in type 2 diabetes patients. However, KB levels have also been reported to be decreased in obesity. We investigated the metabolic adaptation to fasting with respect to glucose and KB metabolism in lean and obese men without type 2 diabetes using stable glucose and D-3HB isotopes in a two-step pancreatic clamp after 38 h of fasting. We found that D-3HB fluxes in the basal state were higher in lean compared to obese men: 15.2 (10.7-27.1) vs. 7.0 (3.5-15.1) micromol/kg lean body mass (LBM) x min, respectively, P ketogenesis. This implies differential insulin sensitivity of intermediary metabolism in obesity.

  9. HCUP Fast Stats

    Data.gov (United States)

    U.S. Department of Health & Human Services — HCUP Fast Stats provides easy access to the latest HCUP-based statistics for health information topics. HCUP Fast Stats uses visual statistical displays in...

  10. Fast food (image)

    Science.gov (United States)

    Fast foods are quick, reasonably priced, and readily available alternatives to home cooking. While convenient and economical for a busy lifestyle, fast foods are typically high in calories, fat, saturated ...

  11. Fast food tips (image)

    Science.gov (United States)

    ... challenge to eat healthy when going to a fast food place. In general, avoiding items that are deep ... challenge to eat healthy when going to a fast food place. In general, avoiding items that are deep ...

  12. High Red Blood Cell Count

    Science.gov (United States)

    Symptoms High red blood cell count By Mayo Clinic Staff A high red blood cell count is an increase in oxygen-carrying cells in your bloodstream. Red blood cells transport oxygen from your lungs to tissues throughout ...

  13. Is fast food addictive?

    Science.gov (United States)

    Garber, Andrea K; Lustig, Robert H

    2011-09-01

    Studies of food addiction have focused on highly palatable foods. While fast food falls squarely into that category, it has several other attributes that may increase its salience. This review examines whether the nutrients present in fast food, the characteristics of fast food consumers or the presentation and packaging of fast food may encourage substance dependence, as defined by the American Psychiatric Association. The majority of fast food meals are accompanied by a soda, which increases the sugar content 10-fold. Sugar addiction, including tolerance and withdrawal, has been demonstrated in rodents but not humans. Caffeine is a "model" substance of dependence; coffee drinks are driving the recent increase in fast food sales. Limited evidence suggests that the high fat and salt content of fast food may increase addictive potential. Fast food restaurants cluster in poorer neighborhoods and obese adults eat more fast food than those who are normal weight. Obesity is characterized by resistance to insulin, leptin and other hormonal signals that would normally control appetite and limit reward. Neuroimaging studies in obese subjects provide evidence of altered reward and tolerance. Once obese, many individuals meet criteria for psychological dependence. Stress and dieting may sensitize an individual to reward. Finally, fast food advertisements, restaurants and menus all provide environmental cues that may trigger addictive overeating. While the concept of fast food addiction remains to be proven, these findings support the role of fast food as a potentially addictive substance that is most likely to create dependence in vulnerable populations.

  14. Next generation red teaming

    CERN Document Server

    Dalziel, Henry

    2015-01-01

    Red Teaming is can be described as a type of wargaming.In private business, penetration testers audit and test organization security, often in a secretive setting. The entire point of the Red Team is to see how weak or otherwise the organization's security posture is. This course is particularly suited to CISO's and CTO's that need to learn how to build a successful Red Team, as well as budding cyber security professionals who would like to learn more about the world of information security. Teaches readers how to dentify systemic security issues based on the analysis of vulnerability and con

  15. Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region

    Directory of Open Access Journals (Sweden)

    Pan Hui-Juan

    2007-09-01

    Full Text Available Abstract Background Giant panda is rare and endangered species endemic to China. The low rates of reproductive success and infectious disease resistance have severely hampered the development of captive and wild populations of the giant panda. The major histocompatibility complex (MHC plays important roles in immune response and reproductive system such as mate choice and mother-fetus bio-compatibility. It is thus essential to understand genetic details of the giant panda MHC. Construction of a bacterial artificial chromosome (BAC library will provide a new tool for panda genome physical mapping and thus facilitate understanding of panda MHC genes. Results A giant panda BAC library consisting of 205,800 clones has been constructed. The average insert size was calculated to be 97 kb based on the examination of 174 randomly selected clones, indicating that the giant panda library contained 6.8-fold genome equivalents. Screening of the library with 16 giant panda PCR primer pairs revealed 6.4 positive clones per locus, in good agreement with an expected 6.8-fold genomic coverage of the library. Based on this BAC library, we constructed a contig map of the giant panda MHC class II region from BTNL2 to DAXX spanning about 650 kb by a three-step method: (1 PCR-based screening of the BAC library with primers from homologous MHC class II gene loci, end sequences and BAC clone shotgun sequences, (2 DNA sequencing validation of positive clones, and (3 restriction digest fingerprinting verification of inter-clone overlapping. Conclusion The identifications of genes and genomic regions of interest are greatly favored by the availability of this giant panda BAC library. The giant panda BAC library thus provides a useful platform for physical mapping, genome sequencing or complex analysis of targeted genomic regions. The 650 kb sequence-ready BAC contig map of the giant panda MHC class II region from BTNL2 to DAXX, verified by the three-step method, offers a

  16. Ramadan, fasting and pregnancy

    DEFF Research Database (Denmark)

    Ahmed, Urfan Zahoor; Lykke, Jacob Alexander

    2014-01-01

    In Islam, the month of Ramadan is a period of fasting lasting 29 or 30 days. Epidemiological studies among Muslims in Denmark have not been conducted, but studies show, that fasting among pregnant Muslim women is common. Fasting does not increase the risk of growth restriction or preterm delivery......, but there are reports of decreased foetal movements. Furthermore, the fasting may have long-term health consequences for the offspring, especially when they reach their middle age. According to Islam and the interpretation, pregnant and breast-feeding women are allowed to postpone the fasting of the month of Ramadan...

  17. Integrative Physiology of Fasting.

    Science.gov (United States)

    Secor, Stephen M; Carey, Hannah V

    2016-03-15

    Extended bouts of fasting are ingrained in the ecology of many organisms, characterizing aspects of reproduction, development, hibernation, estivation, migration, and infrequent feeding habits. The challenge of long fasting episodes is the need to maintain physiological homeostasis while relying solely on endogenous resources. To meet that challenge, animals utilize an integrated repertoire of behavioral, physiological, and biochemical responses that reduce metabolic rates, maintain tissue structure and function, and thus enhance survival. We have synthesized in this review the integrative physiological, morphological, and biochemical responses, and their stages, that characterize natural fasting bouts. Underlying the capacity to survive extended fasts are behaviors and mechanisms that reduce metabolic expenditure and shift the dependency to lipid utilization. Hormonal regulation and immune capacity are altered by fasting; hormones that trigger digestion, elevate metabolism, and support immune performance become depressed, whereas hormones that enhance the utilization of endogenous substrates are elevated. The negative energy budget that accompanies fasting leads to the loss of body mass as fat stores are depleted and tissues undergo atrophy (i.e., loss of mass). Absolute rates of body mass loss scale allometrically among vertebrates. Tissues and organs vary in the degree of atrophy and downregulation of function, depending on the degree to which they are used during the fast. Fasting affects the population dynamics and activities of the gut microbiota, an interplay that impacts the host's fasting biology. Fasting-induced gene expression programs underlie the broad spectrum of integrated physiological mechanisms responsible for an animal's ability to survive long episodes of natural fasting.

  18. Molecular characterization of Rhodococcus equi isolates from horses in Poland: pVapA characteristics and plasmid new variant, 85-kb type V.

    Science.gov (United States)

    Witkowski, Lucjan; Rzewuska, Magdalena; Takai, Shinji; Chrobak-Chmiel, Dorota; Kizerwetter-Świda, Magdalena; Feret, Małgorzata; Gawryś, Marta; Witkowski, Maciej; Kita, Jerzy

    2017-01-26

    Rhodococcus equi is one of the most significant bacterial pathogens affecting foals up to 6 months of age worldwide. Rhodococcosis is present in Poland however information about molecular characterization of R. equi isolates is scarce. This study describes molecular characterization of Rhodococcus equi infection on 13 horse breeding farms in Poland between 2001 and 2012. Samples were collected by tracheobronchial aspiration from pneumonic foals or during necropsy. The R. equi isolates were genotyped by plasmid profiling and pulsed-field gel electrophoresis. Totally, 58 R. equi isolates were investigated. One isolate lost its plasmid. Among the 57 VapA-positive isolates, 48 contained 85-kb type I plasmid (82.8%), 8 contained 87-kb type I plasmid (13.8%). One isolate (1.7%) had a unique restriction cleavage pattern and the 2nd fragment of EcoRI digests of this plasmid DNA was about 2600 bases smaller than that of the 85 kb type I. This new plasmid variant was designated as the "85-kb type V". Among the 58 isolates typeable with VspI-PFGE, ten PFGE clusters were detected. The majority of foals were infected mostly with isolates of low genetic diversity. Most of clinical isolates of R. equi from foals in Poland contain pVapA 85-kb type I and 87-kb type I similarly to the other European countries and the United States. However, the new variant of pVapA 85-kb type V was identified. The chromosomal variability was detected among some of the investigated isolates and the presence of farm-specific isolates might be possible.

  19. FAST User Guide

    Science.gov (United States)

    Walatka, Pamela P.; Clucas, Jean; McCabe, R. Kevin; Plessel, Todd; Potter, R.; Cooper, D. M. (Technical Monitor)

    1994-01-01

    The Flow Analysis Software Toolkit, FAST, is a software environment for visualizing data. FAST is a collection of separate programs (modules) that run simultaneously and allow the user to examine the results of numerical and experimental simulations. The user can load data files, perform calculations on the data, visualize the results of these calculations, construct scenes of 3D graphical objects, and plot, animate and record the scenes. Computational Fluid Dynamics (CFD) visualization is the primary intended use of FAST, but FAST can also assist in the analysis of other types of data. FAST combines the capabilities of such programs as PLOT3D, RIP, SURF, and GAS into one environment with modules that share data. Sharing data between modules eliminates the drudgery of transferring data between programs. All the modules in the FAST environment have a consistent, highly interactive graphical user interface. Most commands are entered by pointing and'clicking. The modular construction of FAST makes it flexible and extensible. The environment can be custom configured and new modules can be developed and added as needed. The following modules have been developed for FAST: VIEWER, FILE IO, CALCULATOR, SURFER, TOPOLOGY, PLOTTER, TITLER, TRACER, ARCGRAPH, GQ, SURFERU, SHOTET, and ISOLEVU. A utility is also included to make the inclusion of user defined modules in the FAST environment easy. The VIEWER module is the central control for the FAST environment. From VIEWER, the user can-change object attributes, interactively position objects in three-dimensional space, define and save scenes, create animations, spawn new FAST modules, add additional view windows, and save and execute command scripts. The FAST User Guide uses text and FAST MAPS (graphical representations of the entire user interface) to guide the user through the use of FAST. Chapters include: Maps, Overview, Tips, Getting Started Tutorial, a separate chapter for each module, file formats, and system

  20. Changes in fatty acid composition of Stenotrophomonas maltophilia KB2 during co-metabolic degradation of monochlorophenols.

    Science.gov (United States)

    Nowak, Agnieszka; Greń, Izabela; Mrozik, Agnieszka

    2016-12-01

    The changes in the cellular fatty acid composition of Stenotrophomonas maltophilia KB2 during co-metabolic degradation of monochlorophenols in the presence of phenol as well as its adaptive mechanisms to these compounds were studied. It was found that bacteria were capable of degrading 4-chlorophenol (4-CP) completely in the presence of phenol, while 2-chlorophenol (2-CP) and 3-chlorophenol (3-CP) they degraded partially. The analysis of the fatty acid profiles indicated that adaptive mechanisms of bacteria depended on earlier exposure to phenol, which isomer they degraded, and on incubation time. In bacteria unexposed to phenol the permeability and structure of their membranes could be modified through the increase of hydroxylated and cyclopropane fatty acids, and straight-chain and hydroxylated fatty acids under 2-CP, 3-CP and 4-CP exposure, respectively. In the exposed cells, regardless of the isomer they degraded, the most important changes were connected with the increase of the contribution of branched fatty acid on day 4 and the content of hydroxylated fatty acids on day 7. The changes, particularly in the proportion of branched fatty acids, could be a good indicator for assessing the progress of the degradation of monochlorophenols by S. maltophilia KB2. In comparison, in phenol-degrading cells the increase of cyclopropane and straight-chain fatty acid content was established. These findings indicated the degradative potential of the tested strain towards the co-metabolic degradation of persistent chlorophenols, and extended the current knowledge about the adaptive mechanisms of these bacteria to such chemicals.

  1. Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.

    Science.gov (United States)

    Nedel, Fernanda; Begnini, Karine; Carvalho, Pedro Henrique de Azambuja; Lund, Rafael Guerra; Beira, Fátima T A; Del Pino, Francisco Augusto B

    2012-11-01

    This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3.

  2. Galaxy Zoo: Passive Red Spirals

    CERN Document Server

    Masters, Karen L; Romer, A Kathy; Nichol, Robert C; Bamford, Steven P; Schawinski, Kevin; Lintott, Chris J; Andreescu, Dan; Campbell, Heather C; Crowcroft, Ben; Doyle, Isabelle; Edmondson, Edward M; Murray, Phil; Raddick, M Jordan; Slosar, Anze; Szalay, Alexander S; Vandenberg, Jan

    2009-01-01

    We study the spectroscopic properties and environments of red spiral galaxies found by the Galaxy Zoo project. By carefully selecting face-on, disk dominated spirals we construct a sample of truly passive disks (not dust reddened, nor dominated by old stellar populations in a bulge). As such, our red spirals represent an interesting set of possible transition objects between normal blue spirals and red early types. We use SDSS data to investigate the physical processes which could have turned these objects red without disturbing their morphology. Red spirals prefer intermediate density regimes, however there are no obvious correlations between red spiral properties and environment - environment alone is not sufficient to determine if a galaxy will become a red spiral. Red spirals are a small fraction of spirals at low masses, but dominate at large stellar masses - massive galaxies are red independent of morphology. We confirm that red spirals have older stellar populations and less recent star formation than ...

  3. Effect of red wine and red grape extract on blood lipids, haemostatic factors, and other risk factors for cardiovascular disease

    DEFF Research Database (Denmark)

    Hansen, Alice Schmidt; Marckmann, P.; Dragsted, L.O.

    2005-01-01

    . Plasma high-density lipoprotein (HDL)- cholesterol (HDL-C), low-density lipoprotein (LDL)-cholesterol (LDL-C), HDL-C/LDL-C-ratio, very-low-density lipoprotein ( VLDL)- triacylglycerol, total cholesterol, fibrinogen, factor VII coagulant activity (FVIIc), blood pressure, and body weight were determined......: 300 ml/day, 38.3 g alcohol/day, female subjects: 200 ml/ day, 25.5 g alcohol/day), 2: water+red grape extract tablets ( wine-equivalent dose), 3: water+red grape extract tablets ( half dose), or 4: water+placebo tablets for a period of 4 weeks. No other sources of alcohol or anthocyanin were allowed...... before and after intervention. Results: Wine consumption was associated with a significant 11 - 16% increase in fasting HDL-C and 8 - 15% decrease in fasting fibrinogen relative to not drinking wine. There were no significant treatment effects on fasting LDL-C, HDL-C/LDL-C-ratio, VLDL...

  4. Red giant seismology: Observations

    Directory of Open Access Journals (Sweden)

    Mosser B.

    2013-03-01

    Full Text Available The CoRoT and Kepler missions provide us with thousands of red-giant light curves that allow a very precise asteroseismic study of these objects. Before CoRoT and Kepler, the red-giant oscillation patterns remained obscure. Now, these spectra are much more clear and unveil many crucial interior structure properties. For thousands of red giants, we can derive from seismic data precise estimates of the stellar mass and radius, the evolutionary status of the giants (with a clear difference between clump and RGB stars, the internal differential rotation, the mass loss, the distance of the stars... Analyzing this amount of information is made easy by the identification of the largely homologous red-giant oscillation patterns. For the first time, both pressure and mixed mode oscillation patterns can be precisely depicted. The mixed-mode analysis allows us, for instance, to probe directly the stellar core. Fine details completing the red-giant oscillation pattern then provide further information on the interior structure, including differential rotation.

  5. Rapidly rotating red giants

    CERN Document Server

    Gehan, Charlotte; Michel, Eric

    2016-01-01

    Stellar oscillations give seismic information on the internal properties of stars. Red giants are targets of interest since they present mixed modes, which behave as pressure modes in the convective envelope and as gravity modes in the radiative core. Mixed modes thus directly probe red giant cores, and allow in particular the study of their mean core rotation. The high-quality data obtained by CoRoT and Kepler satellites represent an unprecedented perspective to obtain thousands of measurements of red giant core rotation, in order to improve our understanding of stellar physics in deep stellar interiors. We developed an automated method to obtain such core rotation measurements and validated it for stars on the red giant branch. In this work, we particularly focus on the specific application of this method to red giants having a rapid core rotation. They show complex spectra where it is tricky to disentangle rotational splittings from mixed-mode period spacings. We demonstrate that the method based on the id...

  6. Comparative azo reductase activity of red azo dyes through caecal and hepatic microsomal fraction in rats.

    Science.gov (United States)

    Singh, S; Das, M; Khanna, S K

    1997-09-01

    In order to study the rate of formation of toxic aromatic amines, anaerobic reduction of four red azo dyes viz. amaranth, carmoisine, fast Red E and ponceau 4R was investigated by incubating caecal content and hepatic microsomal fraction of rats with 37.5 microM concentration of dyes in sodium phosphate buffer pH 7.4 using NADPH generating system, glucose oxidase system and nitrogen as the gaseous phase. Caecal suspension exhibited higher azo reductase activity than that of hepatic microsomal fraction using any of the 4 azo dyes. Caecal microbes showed maximal azo reductase activity when ponceau 4R was used as a substrate followed by fast Red E and carmoisine, while with amaranth the activity was minimum. Similarly ponceau 4 R exhibited maximum hepatic microsomal azo reductase activity followed by fast Red E and carmoisine whereas, amaranth had minimum activity. Caecal flora possessed almost 17 fold higher degradative capability of ponceau 4 R and fast Red E colourants than the hepatic microsomal fraction. The higher reductive ability through caecal flora for ponceau 4R and fast Red E signifies the formation of more aromatic amines which may be re-absorbed through the intestine to be either eliminated through urine as conjugates or retained in the target tissues to elicit toxic effects.

  7. The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

    Directory of Open Access Journals (Sweden)

    Ding Jiabo

    2009-11-01

    Full Text Available Abstract Background Marek's disease virus (MDV has a bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts. By sequencing for the promoters from 8 different strains (CVI988, 814, GA, JM, Md5, G2, RB1B and 648A, it is found, comparing with the other 7 MDV strains, CVI988 has a 5-bp (from -628 to -632 deletion in this region, which caused a Sp1 site destroyed. In order to analysis the activity of the promoter, the complete bi-directional promoters from GA and CVI988 were, respectively, cloned into pCAT-Basic vector in both directions for the recombinants pPGA(pp38-CAT, pPGA(1.8 kb-CAT, pPCVI(pp38-CAT and pPCVI(1.8 kb-CAT. The complete promoter of GA was divided into two single-direction promoters from the replication of MDV genomic DNA, and cloned into pCAT-Basic for pdPGA(pp38-CAT and pdPGA(1.8 kb-CAT as well. The above 6 recombinants were then transfected into chicken embryo fibroblasts (CEFs infected with MDV, and the activity of chloramphenicol acetyltransferase (CAT was measured from the lysed CEFs 48 h post transfection. Results The results showed the activity of the divided promoters was decreased on both directions. In 1.8-kb mRNA direction, it is nearly down to 2.4% (19/781 of the whole promoter, while it keeps 65% (34/52 activity in pp38 direction. The deletion of Sp1 site in CVI988 causes the 20% activity decreased, and has little influence in pp38 direction. Conclusion The present study confirmed their result, and the promoter for the 1.8-kb mRNA transcripts is a much stronger promoter than that in the orientation for pp38.

  8. Little Red Riding Hood

    Institute of Scientific and Technical Information of China (English)

    胡蓉

    2011-01-01

    你一定看过并知道小红帽(Little Red Riding Hood)的故事吧!下面是英语版的小红帽,你看过吗? Once upon a time,people liked a little girl very much.Her red hood(头巾 ) fit her, so people called her Little Red Riding Hood. One day,her mother said, "Take a piece of cake and a bottle ofwine to your grandmother.She is ill and weak.These will do her good.Donot run off the road,or you may fall and break the bottle,and then yourgrandmother will get nothing.OK! Go at once."

  9. RED-ML

    DEFF Research Database (Denmark)

    Xiong, Heng; Liu, Dongbing; Li, Qiye

    2017-01-01

    roles can be further understood. However, a major barrier that prevents RNA editing from being a routine RNA-seq analysis, similar to gene expression and splicing analysis, for example, is the lack of user-friendly and effective computational tools. Based on years of experience of analyzing RNA editing...... using diverse RNA-seq datasets, we have developed a software tool, RED-ML: RNA Editing Detection based on Machine learning (pronounced as "red ML"). The input to RED-ML can be as simple as a single BAM file, while it can also take advantage of matched genomic variant information when available...... accurately detect novel RNA editing sites without relying on curated RNA editing databases. We have also made this tool freely available via GitHub . We have developed a highly accurate, speedy and general-purpose tool for RNA editing detection using RNA-seq data...

  10. Fast growth in control

    NARCIS (Netherlands)

    Z. Rico (Zulay)

    2009-01-01

    textabstractThe focus of this paper is on the influence of the fast growth of organizations on the design process of management control systems. What are the management accounting and control problems that a fast growth organization encounters that can be ascribed to this growth. What are the circum

  11. Fast protein folding kinetics

    Science.gov (United States)

    Gelman, Hannah; Gruebele, Martin

    2014-01-01

    Fast folding proteins have been a major focus of computational and experimental study because they are accessible to both techniques: they are small and fast enough to be reasonably simulated with current computational power, but have dynamics slow enough to be observed with specially developed experimental techniques. This coupled study of fast folding proteins has provided insight into the mechanisms which allow some proteins to find their native conformation well less than 1 ms and has uncovered examples of theoretically predicted phenomena such as downhill folding. The study of fast folders also informs our understanding of even “slow” folding processes: fast folders are small, relatively simple protein domains and the principles that govern their folding also govern the folding of more complex systems. This review summarizes the major theoretical and experimental techniques used to study fast folding proteins and provides an overview of the major findings of fast folding research. Finally, we examine the themes that have emerged from studying fast folders and briefly summarize their application to protein folding in general as well as some work that is left to do. PMID:24641816

  12. Ramadan, faste og graviditet

    DEFF Research Database (Denmark)

    Ahmed, Urfan Zahoor; Lykke, Jacob Alexander

    2014-01-01

    , but there are reports of decreased foetal movements. Furthermore, the fasting may have long-term health consequences for the offspring, especially when they reach their middle age. According to Islam and the interpretation, pregnant and breast-feeding women are allowed to postpone the fasting of the month of Ramadan...

  13. Fast protein folding kinetics.

    Science.gov (United States)

    Gelman, Hannah; Gruebele, Martin

    2014-05-01

    Fast-folding proteins have been a major focus of computational and experimental study because they are accessible to both techniques: they are small and fast enough to be reasonably simulated with current computational power, but have dynamics slow enough to be observed with specially developed experimental techniques. This coupled study of fast-folding proteins has provided insight into the mechanisms, which allow some proteins to find their native conformation well fast folders also informs our understanding of even 'slow' folding processes: fast folders are small; relatively simple protein domains and the principles that govern their folding also govern the folding of more complex systems. This review summarizes the major theoretical and experimental techniques used to study fast-folding proteins and provides an overview of the major findings of fast-folding research. Finally, we examine the themes that have emerged from studying fast folders and briefly summarize their application to protein folding in general, as well as some work that is left to do.

  14. Fast food purchasing and access to fast food restaurants: a multilevel analysis of VicLANES.

    Science.gov (United States)

    Thornton, Lukar E; Bentley, Rebecca J; Kavanagh, Anne M

    2009-05-27

    While previous research on fast food access and purchasing has not found evidence of an association, these studies have had methodological problems including aggregation error, lack of specificity between the exposures and outcomes, and lack of adjustment for potential confounding. In this paper we attempt to address these methodological problems using data from the Victorian Lifestyle and Neighbourhood Environments Study (VicLANES) - a cross-sectional multilevel study conducted within metropolitan Melbourne, Australia in 2003. The VicLANES data used in this analysis included 2547 participants from 49 census collector districts in metropolitan Melbourne, Australia. The outcome of interest was the total frequency of fast food purchased for consumption at home within the previous month (never, monthly and weekly) from five major fast food chains (Red Rooster, McDonalds, Kentucky Fried Chicken, Hungry Jacks and Pizza Hut). Three measures of fast food access were created: density and variety, defined as the number of fast food restaurants and the number of different fast food chains within 3 kilometres of road network distance respectively, and proximity defined as the road network distance to the closest fast food restaurant.Multilevel multinomial models were used to estimate the associations between fast food restaurant access and purchasing with never purchased as the reference category. Models were adjusted for confounders including determinants of demand (attitudes and tastes that influence food purchasing decisions) as well as individual and area socio-economic characteristics. Purchasing fast food on a monthly basis was related to the variety of fast food restaurants (odds ratio 1.13; 95% confidence interval 1.02 - 1.25) after adjusting for individual and area characteristics. Density and proximity were not found to be significant predictors of fast food purchasing after adjustment for individual socio-economic predictors. Although we found an independent

  15. Fast food purchasing and access to fast food restaurants: a multilevel analysis of VicLANES

    Directory of Open Access Journals (Sweden)

    Kavanagh Anne M

    2009-05-01

    Full Text Available Abstract Background While previous research on fast food access and purchasing has not found evidence of an association, these studies have had methodological problems including aggregation error, lack of specificity between the exposures and outcomes, and lack of adjustment for potential confounding. In this paper we attempt to address these methodological problems using data from the Victorian Lifestyle and Neighbourhood Environments Study (VicLANES – a cross-sectional multilevel study conducted within metropolitan Melbourne, Australia in 2003. Methods The VicLANES data used in this analysis included 2547 participants from 49 census collector districts in metropolitan Melbourne, Australia. The outcome of interest was the total frequency of fast food purchased for consumption at home within the previous month (never, monthly and weekly from five major fast food chains (Red Rooster, McDonalds, Kentucky Fried Chicken, Hungry Jacks and Pizza Hut. Three measures of fast food access were created: density and variety, defined as the number of fast food restaurants and the number of different fast food chains within 3 kilometres of road network distance respectively, and proximity defined as the road network distance to the closest fast food restaurant. Multilevel multinomial models were used to estimate the associations between fast food restaurant access and purchasing with never purchased as the reference category. Models were adjusted for confounders including determinants of demand (attitudes and tastes that influence food purchasing decisions as well as individual and area socio-economic characteristics. Results Purchasing fast food on a monthly basis was related to the variety of fast food restaurants (odds ratio 1.13; 95% confidence interval 1.02 – 1.25 after adjusting for individual and area characteristics. Density and proximity were not found to be significant predictors of fast food purchasing after adjustment for individual socio

  16. Fast ejendom III

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    Bogen er det tredje bind af tre planlagte bind om fast ejendom: I Overdragelsen, II Bolighandlen og III Ejerbeføjelsen. Fremstillingens giver et grundigt overblik over centrale områder af en omfattende regulering af fast ejendom, med angivelse af litteratur, hvor læseren kan søge yderligere...... oplysning. En ejer af fast ejendom er på særdeles mange områder begrænset i sin råden sammenlignet med ejeren af et formuegode i almindelighed. Fremstillingen tager udgangspunkt i ejerens perspektiv (fremfor samfundets eller myndighedernes). Både den privatretlige og offentligretlige regulering behandles......, eksempelvis ejendomsdannelsen, servitutter, naboretten, hævd, zoneinddelingen, den fysiske planlægning, beskyttelse af natur, beskyttelse af kultur, forurening fra fast ejendom, erstatning for forurening, jordforurening, ekspropriation, byggeri og adgang til fast ejendom....

  17. Islamic Fasting and Diabetes

    Directory of Open Access Journals (Sweden)

    Fereidoun Azizi

    2013-07-01

    Full Text Available The aim of this article is to review health-related aspects of Ramadan fasting in normal individuals and diabetics. During fasting days of Ramadan, glucose homeostasis is maintained by meal taken bepore dawn and by liver glycogen stores. Changes in serum lipids are variable and defend on the quality and quantity of food consumption and changes in weight. Compliant, well controlled type 2 diabetics may observe Ramadan fasting; but fasting is not recommended for type 1, non complaint, poorly controlled and pregnant diabetics. Although Ramadan fasting is safe for all healthy individuals and well controlled diabetics, those with uncontrolled diabetics and diabetics with complications should consult physicians and follow scientific recommendations.

  18. Fast Spectrum Reactors

    CERN Document Server

    Todd, Donald; Tsvetkov, Pavel

    2012-01-01

    Fast Spectrum Reactors presents a detailed overview of world-wide technology contributing to the development of fast spectrum reactors. With a unique focus on the capabilities of fast spectrum reactors to address nuclear waste transmutation issues, in addition to the well-known capabilities of breeding new fuel, this volume describes how fast spectrum reactors contribute to the wide application of nuclear power systems to serve the global nuclear renaissance while minimizing nuclear proliferation concerns. Readers will find an introduction to the sustainable development of nuclear energy and the role of fast reactors, in addition to an economic analysis of nuclear reactors. A section devoted to neutronics offers the current trends in nuclear design, such as performance parameters and the optimization of advanced power systems. The latest findings on fuel management, partitioning and transmutation include the physics, efficiency and strategies of transmutation, homogeneous and heterogeneous recycling, in addit...

  19. Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2014-03-01

    Full Text Available Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb. As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan

  20. Localization of the familial Mediterranean fever gene (FMF) to a 250-kb interval in non-Ashkenazi Jewish founder haplotypes

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-09-01

    Chromosome 16p13.3 harbors a gene (MEF) associated with familial Mediterranean fever (FMF), a recessive disease very common in populations of Mediterranean ancestry. In the course of positional cloning of MEF, we genotyped 26 non-Ashkenazi Jewish FMF pedigrees (310 meioses) with 15 microsatellite markers, most of which were recently developed by Genethon. Identification of recombination events in the haplotypes allowed narrowing of the MEF interval to a region between D16S3124 (telomeric) and D16S475 (centromeric). Two markers, D16S3070 and D16S3275, a microsatellite marker isolated from a YAC that also contains D16S3070, showed no recombination with the disease. Linkage disequilibrium and haplotype analysis high-lighted the existence of a founder haplotype in our population. The core ancestral alleles were present in 71% of MEF-bearing chromosomes at loci D16S3070 and D16S3275. Furthermore, identification of historical crossing-over events in these pedigrees indicated that MEF is located between these two loci, which are both contained in a 250-kb genomic fragment. 24 refs., 4 figs., 3 tabs.

  1. Translocation breakpoint maps 5 kb 3' from TWIST in a patient affected with Saethre-Chotzen syndrome.

    Science.gov (United States)

    Krebs, I; Weis, I; Hudler, M; Rommens, J M; Roth, H; Scherer, S W; Tsui, L C; Füchtbauer, E M; Grzeschik, K H; Tsuji, K; Kunz, J

    1997-07-01

    Saethre-Chotzen syndrome, a common autosomal dominant craniosynostosis in humans, is characterized by brachydactyly, soft tissue syndactyly and facial dysmorphism including ptosis, facial asymmetry, and prominent ear crura. Previously, we identified a yeast artificial chromosome that encompassed the breakpoint of an apparently balanced t(6;7) (q16.2;p15.3) translocation associated with a mild form of Saethre-Chotzen syndrome. We now describe, at the DNA sequence level, the region on chromosome 7 affected by this translocation event. The rearrangement occurred approximately 5 kb 3' of the human TWIST locus and deleted 518 bp of chromosome 7. The TWIST gene codes for a transcription factor containing a basic helix-loop-helix (b-HLH) motif and has recently been described as a candidate gene for Saethre-Chotzen syndrome, based on the detection of mutations within the coding region. Potential exon sequences flanking the chromosome 7 translocation breakpoint did not hit known genes in database searches. The chromosome rearrangement downstream of TWIST is compatible with the notion that this is a Saethre-Chotzen syndrome gene and implies loss of function of one allele by a positional effect as a possible mechanism of mutation to evoke the syndrome.

  2. Amine basicity (pKb) controls the analyte binding energy on single walled carbon nanotube electronic sensor arrays.

    Science.gov (United States)

    Lee, Chang Young; Strano, Michael S

    2008-02-06

    A wide range of analytes adsorb irreversibly to the surfaces of single walled carbon nanotube electronic networks typically used as sensors or thin-film transistors, although to date, the mechanism is not understood. Using thionyl chloride as a model electron-withdrawing adsorbate, we show that reversible adsorption sites can be created on the nanotube array via noncovalent functionalization with amine-terminated molecules of pKa nanotube network comprising single, largely unbundled nanotubes, near the electronic percolation threshold is required for the effective conversion to a reversibly binding array. By examining 11 types of amine-containing molecules, we show that analyte adsorption is largely affected by the basicity (pKb) of surface groups. The binding energy of the analyte is apparently reduced by its adsorption on the surface chemical groups instead of directly on the SWNT array itself. This mediated adsorption mechanism is supported by X-ray photoelectron spectroscopy (XPS) and molecular potential calculations. Reversible detection with no active regeneration at the parts-per-trillion level is demonstrated for the first time by creating a higher adsorption site density with a polymer amine, such as polyethyleneimine (PEI). Last, we demonstrate that this transition to reversibility upon surface functionalization is a general phenomenon.

  3. Red Emitting VCSEL

    Science.gov (United States)

    Jetter, Michael; Roßbach, Robert; Michler, Peter

    This chapter describes the progress in development of vertical-cavity surface-emitting lasers (VCSEL) emitting in the red spectral region around 650 nm for data transmission over polymer optical fibers (POF). First, growth issues of red VCSEL using two different material systems, namely AlGaAs and AlGaInP, are introduced. In particular, the optical and electrical state-of-the-art characteristics as low threshold currents ({≤} 1 mA) and high output powers (several mW) are presented with a special focus on emission wavelength. Also the thermal budget and heat removal in the devices are pointed out with regard to the geometry of the VCSEL. Small-signal modulation response in terms of maximum resonance frequency in dependance on temperature behavior are discussed. Applications of these devices in optical interconnects are described and digital data transmission at data rates up to 2.1 Gbit/s over step-index POF is reported. These properties make red emitting VCSEL perfectly suited for high-speed low power consuming light sources for optical data communication via POF. By introducing InP quantum dots as gain material in red emitting VCSEL nearly temperature independent record low threshold current densities of around 10 A/cm2 could be observed.

  4. 'Vintage' Red Raspberry

    Science.gov (United States)

    'Vintage' is a new primocane-fruiting red raspberry (Rubus idaeus L.) from the U.S. Dept. of Agriculture–Agricultural Research Service (USDA–ARS) breeding program in Corvallis, OR released in cooperation with the Oregon State Agricultural Experiment Station and the Washington State University Agricu...

  5. Red Mud Stacking

    Science.gov (United States)

    Bélanger, Marie-J.

    The red mud slurry "stacking" method used in many Alcan Plants has been developed in the 1980's. The aim of this technique is to use minimum space for the disposal of the residue and to rapidly obtain consolidated material. The consistency of the mud slurry plays a key role in the steepness (angle) of the stacking slope.

  6. 21-cm Intensity Mapping with FAST

    CERN Document Server

    Smoot, George F

    2014-01-01

    This paper describes a program to map large-scale cosmic structures on the largest possible scales by using FAST\\cite{Nan:2011} to make a 21-cm (red-shifted) Intensity Map of the sky for the range $0.5 < z < 2.5$. The goal is to map to the angular and spectral resolution of FAST a large swath of the sky by simple drift scans with a transverse set of beams. This approach would be competitive to galaxy surveys and could be completed before SKA could begin a more detailed and precise effort. The science would be to measure the large-scale structure on the size of the baryon acoustic oscillations and larger scale and the results would be competitive to its contemporary observations and signficant. The survey would be uniquely sensitive to the potential very large-scale features from GUT-scale Inflation and complementary to the CMB observations.

  7. 3,3′,4,4′,5-Pentachlorobiphenyl Inhibits Drug Efflux Through P-Glycoprotein in KB-3 Cells Expressing Mutant Human P-Glycoprotein

    Directory of Open Access Journals (Sweden)

    Hiroshi Fujise

    2004-01-01

    Full Text Available The effects on the drug efflux of 3,3′,4,4′,5-pentachlorobiphenyl (PCB-126, the most toxic of all coplanar polychlorinated biphenyls (Co-PCBs, were examined in KB-3 cells expressing human wild-type and mutant P-glycoprotein in which the 61st amino acid was substituted for serine or phenylalanine (KB3-Phe61. In the cells expressing P-glycoproteins, accumulations of vinblastine and colchicine decreased form 85% to 92% and from 62% to 91%, respectively, and the drug tolerances for these chemicals were increased. In KB3-Phe61, the decreases in drug accumulation were inhibited by adding PCB-126 in a way similar to that with cyclosporine A: by adding 1 μM PCB-126, the accumulations of vinblastine and colchicine increased up to 3.3- and 2.3-fold, respectively. It is suggested that PCB-126 decreased the drug efflux by inhibiting the P-glycoprotein in KB3-Phe61. Since there were various P-glycoproteins and many congeners of Co-PCBs, this inhibition has to be considered a new cause of the toxic effects of Co-PCBs.

  8. Astragalus polysaccharides suppress ICAM-1 and VCAM-1 expression in TNF-α-treated human vascular endothelial cells by blocking NF-KB activation

    Institute of Scientific and Technical Information of China (English)

    Yu-ping ZHU; Tao SHEN; Ya-jun LIN; Bei-dong CHEN; Yang RUAN; Yuan CAO; Yue QIAO

    2013-01-01

    Aim:To investigate the effects ofAstragalus polysaccharides (APS) on tumor necrosis factor (TNF)-α-induced inflammatory reactions in human umbilical vein endothelial cells (HUVECs) and to elucidate the underlying mechanisms.Methods:HUVECs were treated with TNF-α for 24 h.The amounts of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) were determined with Western blotting.HUVEC viability and apoptosis were detected using cell viability assay and Hoechst staining,respectively.Reactive oxygen species (ROS) production was measured by DHE staining.Monocyte and HUVEC adhesion assay was used to detect endothelial cell adhesive function.NF-KB activation was detected with immunofluorescence.Results:TNF-α (1-80 ng/mL) caused dose-and time-dependent increases of ICAM-1 and VCAM-1 expression in HUVECs,accompanied by significant augmentation of IKB phosphorylation and NF-KB translocation into the nuclei.Pretreatment with APS (10 and 50 μg/mL)significantly attenuated TNFα-induced upregulation of ICAM-1,VCAM-1,and NF-KB translocation.Moreover,APS significantly reduced apoptosis,ROS generation and adhesion function damage in TNF-α-treated HUVECs.Conclusion:APS suppresses TNFα-induced adhesion molecule expression by blocking NF-KB signaling and inhibiting ROS generation in HUVECs.The results suggest that APS may be used to treat and prevent endothelial cell injury-related diseases.

  9. A potential contributory role for ciliary dysfunction in the 16p11.2 600 kb BP4-BP5 pathology

    NARCIS (Netherlands)

    Migliavacca, Eugenia; Golzio, Christelle; Männik, Katrin; Blumenthal, Ian; Oh, Edwin C.; Harewood, Louise; Kosmicki, Jack A.; Loviglio, Maria Nicla; Giannuzzi, Giuliana; Hippolyte, Loyse; Maillard, Anne M.; Alfaiz, Ali Abdullah; Witwicki, Robert; Didelot, Gérard; Van Der Werf, Ilse; Alfaiz, Ali A.; Zazhytska, Marianna; Chrast, Jacqueline; Macé, Aurélien; Bergmann, Sven; Kutalik, Zoltan; Siffredi, Vanessa; Zufferey, Flore; Martinet, Danielle; Bena, Frédérique; Rauch, Anita; Bouquillon, Sonia; Delobel, Bruno; Boute, Odile; Duban-Bedu, Bénédicte; Le Caignec, Cédric; Isidor, Bertrand; Chiesa, Jean; Keren, Boris; Gilbert-Dussardier, Brigitte; Touraine, Renaud; Campion, Dominique; Thambo, Caroline Rooryck; Mathieu-Dramard, Michèle; Plessis, Ghislaine; Kooy, Frank; Peeters, Hilde; Ounap, Katrin; Vulto-Van Silfhout, Anneke T.; De Vries, Bert B.; Van Binsbergen, Ellen; Nordgren, Ann; Mucciolo, Mafalda; Renieri, Alessandra; Rajcan-Separovic, Evica; Philipps, John A.; Ellis, Richard J.; Van Haelst, Mieke M.; Andrieux, Joris; Gusella, James F.; Daly, Mark J.; Beckmann, Jacques S.; Jacquemont, Sébastien; Talkowski, Michael E.; Katsanis, Nicholas; Reymond, Alexandre

    2015-01-01

    The 16p11.2 600 kb copy-number variants (CNVs) are associated with mirror phenotypes on BMI, head circumference, and brain volume and represent frequent genetic lesions in autism spectrum disorders (ASDs) and schizophrenia. Here we interrogated the transcriptome of individuals carrying reciprocal 16

  10. Identification and gene prediction of a 24 kb region containing xa5, a recessive bacterial blight resistance gene in rice (Oryza sativa L.)

    Institute of Scientific and Technical Information of China (English)

    ZHONG Yiming; JIANG Guanghuai; CHEN Xuewei; XIA Zhihui; LI Xiaobing; ZHU Lihuang; ZHAI Wenxue

    2003-01-01

    Rice xa5 gene provides recessive, race-specific resistance to bacterial blight disease caused by the pathogen Xanthomonas oryzae pv. oryzae and has great value for research and breeding. In an effort to clone xa5, an F2 population of 4892 individuals was developed from the xa5 near isogenic lines, IR24 and IRBB5. A fine mapping procedure was conducted and tightly linked RFLP markers were used to screen a BAC library of IRBB56, a resistant rice line containing the xa5 gene. A 213 kb contig covering the xa5 locus was constructed. According to the sequences from the International Rice Genome Sequening Project (IRGSP), the Chinese Superhybrid Rice Genome Project (SRGP) and some sub-clones of the contig, twelve SSLP and CAPS markers were developed for fine mapping. The xa5 gene was mapped to a 0.3 cM interval between markers K5 and T4, which spanned an interval of approximately 24 kb, co-segregating with marker T2. Sequence analysis of the 24 kb region revealed that an ABC transporter and a basal transcription factor (TFIIa) were potential candidates for the xa5 resistance gene product. The molecular mechanism by which the xa5 gene provides recessive, race-specific resistance to bacterial blight will be elucidated by the functional tests of the 24 kb DNA and the candidate genes.

  11. FAST Construction Progress

    Science.gov (United States)

    Nan, R. D.; Zhang, H. Y.; Zhang, Y.; Yang, L.; Cai, W. J.; Liu, N.; Xie, J. T.; Zhang, S. X.

    2016-11-01

    The Five-hundred-meter Aperture Spherical radio Telescope (FAST) is a Chinese mega-science project to build the largest single dish radio telescope in the world. A unique karst depression in Guizhou province has been selected as the site to build an active reflector radio telescope with a diameter of 500 m and three outstanding aspects, which enables FAST to have a large sky coverage and the ability of observing astronomical targets with a high precision. Chinese Academy of Sciences and Guizhou province are in charge of FAST construction. The first light of the telescope was expected on September 25, 2016.

  12. Fast Breeder Reactor studies

    Energy Technology Data Exchange (ETDEWEB)

    Till, C.E.; Chang, Y.I.; Kittel, J.H.; Fauske, H.K.; Lineberry, M.J.; Stevenson, M.G.; Amundson, P.I.; Dance, K.D.

    1980-07-01

    This report is a compilation of Fast Breeder Reactor (FBR) resource documents prepared to provide the technical basis for the US contribution to the International Nuclear Fuel Cycle Evaluation. The eight separate parts deal with the alternative fast breeder reactor fuel cycles in terms of energy demand, resource base, technical potential and current status, safety, proliferation resistance, deployment, and nuclear safeguards. An Annex compares the cost of decommissioning light-water and fast breeder reactors. Separate abstracts are included for each of the parts.

  13. Gas cooled fast reactor

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1972-06-01

    Although most of the development work on fast breeder reactors has been devoted to the use of liquid metal cooling, interest has been expressed for a number of years in alternative breeder concepts using other coolants. One of a number of concepts in which interest has been retained is the Gas-Cooled Fast Reactor (GCFR). As presently envisioned, it would operate on the uranium-plutonium mixed oxide fuel cycle, similar to that used in the Liquid Metal Fast Breeder Reactor (LMFBR), and would use helium gas as the coolant.

  14. Red Blood Cell Antibody Identification

    Science.gov (United States)

    ... ID, RBC; RBC Ab ID Formal name: Red Blood Cell Antibody Identification Related tests: Direct Antiglobulin Test ; RBC ... I should know? How is it used? Red blood cell (RBC) antibody identification is used as a follow- ...

  15. Rare red blood cell abnormalities

    NARCIS (Netherlands)

    van Zwieten, R.

    2015-01-01

    The aim of this thesis is to give insight in the process of diagnosing rare red blood cell defects, to clarify the relation of a defect with cell function and to extend, in this respect, our knowledge about normal red cell function and biochemistry. It is possible to categorize different red cell ab

  16. FAST joins Breakthrough programme

    Science.gov (United States)

    Banks, Michael

    2016-11-01

    The 180m Five-hundred-meter Aperture Spherical radio Telescope (FAST) - the world's largest single-aperture radio receiver - has become part of the Breakthrough Listen programme, which launched in July 2015 to look for intelligent life beyond Earth.

  17. CMS Fast Facts

    Data.gov (United States)

    U.S. Department of Health & Human Services — CMS has developed a new quick reference statistical summary on annual CMS program and financial data. CMS Fast Facts includes summary information on total program...

  18. FAST Maser Surveys

    Indian Academy of Sciences (India)

    J. S. Zhang

    2014-09-01

    FAST, the Five-hundred meter Aperture Spherical radio Telescope, will become the largest operating single-dish telescope in the coming years. It has many advantages: much better sensitivity for its largest collecting area; large sky coverage due to its innovative design of the active primary surface; extremely radio quiet from its unique location, etc. In this work, I will highlight the future capabilities of FAST to discover and observe both galactic and extragalactic masers.

  19. Three classes of plasmid (47-63 kb) carry the type B neurotoxin gene cluster of group II Clostridium botulinum.

    Science.gov (United States)

    Carter, Andrew T; Austin, John W; Weedmark, Kelly A; Corbett, Cindi; Peck, Michael W

    2014-08-01

    Pulsed-field gel electrophoresis and DNA sequence analysis of 26 strains of Group II (nonproteolytic) Clostridium botulinum type B4 showed that 23 strains carried their neurotoxin gene cluster on a 47-63 kb plasmid (three strains lacked any hybridization signal for the neurotoxin gene, presumably having lost their plasmid). Unexpectedly, no neurotoxin genes were found on the chromosome. This apparent constraint on neurotoxin gene transfer to the chromosome stands in marked contrast to Group I C. botulinum, in which neurotoxin gene clusters are routinely found in both locations. The three main classes of type B4 plasmid identified in this study shared different regions of homology, but were unrelated to any Group I or Group III plasmid. An important evolutionary aspect firmly links plasmid class to geographical origin, with one class apparently dominant in marine environments, whereas a second class is dominant in European terrestrial environments. A third class of plasmid is a hybrid between the other two other classes, providing evidence for contact between these seemingly geographically separated populations. Mobility via conjugation has been previously demonstrated for the type B4 plasmid of strain Eklund 17B, and similar genes associated with conjugation are present in all type B4 plasmids now described. A plasmid toxin-antitoxin system pemI gene located close to the neurotoxin gene cluster and conserved in each type B4 plasmid class may be important in understanding the mechanism which regulates this unique and unexpected bias toward plasmid-borne neurotoxin genes in Group II C. botulinum type B4.

  20. A Meiotic Drive Element in the Maize Pathogen Fusarium verticillioides Is Located Within a 102 kb Region of Chromosome V.

    Science.gov (United States)

    Pyle, Jay; Patel, Tejas; Merrill, Brianna; Nsokoshi, Chabu; McCall, Morgan; Proctor, Robert H; Brown, Daren W; Hammond, Thomas M

    2016-08-09

    Fusarium verticillioides is an agriculturally important fungus because of its association with maize and its propensity to contaminate grain with toxic compounds. Some isolates of the fungus harbor a meiotic drive element known as Spore killer (Sk(K)) that causes nearly all surviving meiotic progeny from an Sk(K) × Spore killer-susceptible (Sk(S)) cross to inherit the Sk(K) allele. Sk(K) has been mapped to chromosome V but the genetic element responsible for meiotic drive has yet to be identified. In this study, we used cleaved amplified polymorphic sequence markers to genotype individual progeny from an Sk(K) × Sk(S) mapping population. We also sequenced the genomes of three progeny from the mapping population to determine their single nucleotide polymorphisms. These techniques allowed us to refine the location of Sk(K) to a contiguous 102 kb interval of chromosome V, herein referred to as the Sk region. Relative to Sk(S) genotypes, Sk(K) genotypes have one extra gene within this region for a total of 42 genes. The additional gene in Sk(K) genotypes, herein named SKC1 for Spore Killer Candidate 1, is the most highly expressed gene from the Sk region during early stages of sexual development. The Sk region also has three hyper-variable regions, the longest of which includes SKC1 The possibility that SKC1, or another gene from the Sk region, is an essential component of meiotic drive and spore killing is discussed.

  1. Epigallocatechin-3-gallate regulates cell growth, cell cycle and phosphorylated nuclear factor-KB in human dermal fibroblasts

    Institute of Scientific and Technical Information of China (English)

    Dong-Wook HAN; Mi Hee LEE; Hak Hee KIM; Suong-Hyu HYON; Jong-Chul PARK

    2011-01-01

    Aim: To investigate the effects of (-)epigallocatechin-3-gallate (EGCG), the main polyphenol in green tea, on cell growth, cell cycle and phosphorylated nuclear factor-kB (pNF-KB) expression in neonatal human dermal fibroblasts (nHDFs).Methods: The proliferation and cell-cycle of nHDFs were determined using WST-8 cell growth assay and flow cytometry, respectively. The apoptosis was examined using DNA ladder and Annexin V-FITC assays. The expression levels of pNF-kB and cell cycle-related genes and proteins in nHDFs were measured using cDNA microarray analyses and Western blot. The cellular uptake of EGCG was examined using fluorescence (FITC)-Iabeled EGCG (FITC-EGCG) in combination with confocal microscopy.Results: The effect of EGCG on the growth of nHDFs depended on the concentration tested. At a low concentration (200 μmol/L), EGCG resulted in a slight decrease in the proportion of ceils in the S and G/M phases of cell cycle with a concomitant increase in the proportion of cells in G/G phase. At the higher doses (400 and 800 pmol/L), apoptosis was induced. The regulation of EGCG on the expression of pNF-kB was also concentration-dependent, whereas it did not affect the unphosphorylated NF-kB expression, cDNA microarray analysis showed that cell cycle-related genes were down-regulated by EGCG (200 μmol/L). The expression of cyclins A/B and cyclin-dependent kinase 1 was reversibly regulated by EGCG (200 μmol/L). FITC-EGCG was found to be internalized into the cyto-plasm and translocated into the nucleus of nHDFs.Conclusion: EGCG, through uptake into cytoplasm, reversibly regulated the cell growth and expression of cell cycle-related proteins and genes in normal fibroblasts.

  2. Red Velvet Be Natural

    Institute of Scientific and Technical Information of China (English)

    2014-01-01

    各种魅力全消化最近,以翻唱曲《Be Natural》展开回归活动的S.M新女团Red Velvet,在此次新歌中,展现了和出道曲《Happiness》中活泼可爱样子截然相反的成熟干练形象,如此极速的反差,吸引了大家的注意。作为一个刚出道的新人组合,Red Velvet此次不仅通过椅子舞表演完美地完成了高难度的打歌舞台,甚至在现场,也为大家展现了不输任何人的LIVE表演。

  3. Fasting and Urinary Stones

    Directory of Open Access Journals (Sweden)

    Ali Shamsa

    2013-11-01

    Full Text Available Introduction: Fasting is considered as one of the most important practices of Islam, and according to Prophet Mohammad, fasting is obligatory upon Muslims. The aim of this study is to evaluate the effects of fasting on urinary stones. Materials and Methods: Very few studies have been carried out on urinary stones and the effect of Ramadan fasting. The sources of the present study are Medline and articles presented by local and Muslim researchers. Meanwhile, since we are acquainted with three well-known researchers in the field of urology, we contacted them via email and asked for their professional opinions. Results: The results of studies about the relationship of urinary stones and their incidence in Ramadan are not alike, and are even sometimes contradictory. Some believe that increased incidence of urinary stones in Ramadan is related not to fasting, but to the rise of weather temperature in hot months, and an increase in humidity. Conclusion: Numerous biological and behavioral changes occur in people who fast in Ramadan and some researchers believe that urinary stone increases during this month.

  4. Fasting and urinary stones

    Directory of Open Access Journals (Sweden)

    Ali Shamsa

    2013-12-01

    Full Text Available Introduction: Fasting is considered as one of the most important practices of Islam, and according to Prophet Mohammad, fasting is obligatory upon Muslims. The aim of this study is to evaluate the effects of fasting on urinary stones. Materials and Methods:Very few studies have been carried out on urinary stones and the effect of Ramadan fasting. The sources of the present study are Medline and articles presented by local and Muslim researchers. Meanwhile, since we are acquainted with three well-known researchers in the field  of urology, we contacted them via email and asked for their professional opinions. Results:The results of studies about the relationship of urinary stones and their incidence in Ramadan are not alike, and are even sometimes contradictory. Some believe that increased incidence of urinary stones in Ramadan is related not to fasting, but to the rise of weather temperature in hot months, and an increase in humidity. Conclusion: Numerous biological and behavioral changes occur in people who fast in Ramadan and some researchers believe that urinary stone increases during this month.

  5. Great Red Spot (GRS)

    Science.gov (United States)

    Murdin, P.

    2000-11-01

    A huge permanent anticyclone in Jupiter's southern hemisphere, visible as a reddish oval at just over 20 °S. The earliest unequivocal observation was by Heinrich Schwabe in 1831 (the often-quoted sighting by Robert Hooke in 1664 now seems to have been of a similar but different spot). The GRS became a striking feature around 1880, when it developed a deep red coloration. It was also prominent in ...

  6. Chaotic Red Queen coevolution in three-species food chains

    Science.gov (United States)

    Dercole, Fabio; Ferriere, Regis; Rinaldi, Sergio

    2010-01-01

    Coevolution between two antagonistic species follows the so-called ‘Red Queen dynamics’ when reciprocal selection results in an endless series of adaptation by one species and counteradaptation by the other. Red Queen dynamics are ‘genetically driven’ when selective sweeps involving new beneficial mutations result in perpetual oscillations of the coevolving traits on the slow evolutionary time scale. Mathematical models have shown that a prey and a predator can coevolve along a genetically driven Red Queen cycle. We found that embedding the prey–predator interaction into a three-species food chain that includes a coevolving superpredator often turns the genetically driven Red Queen cycle into chaos. A key condition is that the prey evolves fast enough. Red Queen chaos implies that the direction and strength of selection are intrinsically unpredictable beyond a short evolutionary time, with greatest evolutionary unpredictability in the superpredator. We hypothesize that genetically driven Red Queen chaos could explain why many natural populations are poised at the edge of ecological chaos. Over space, genetically driven chaos is expected to cause the evolutionary divergence of local populations, even under homogenizing environmental fluctuations, and thus to promote genetic diversity among ecological communities over long evolutionary time. PMID:20356888

  7. FastStats: Women's Health

    Science.gov (United States)

    ... PDF – 926 KB] The Association of Marital Status and Offers of Employer-Based Health Insurance Coverage for Employed Women Aged 27–64: United States, 2014–2015 Use of Family Planning and Related Medical Services among Women Aged ...

  8. The remarkable Red Rectangle: A Stairway to Heaven?

    Science.gov (United States)

    2004-05-01

    HD 44179 Nebula hi-res Size hi-res: 865 Kb Credits: NASA/ESA, Hans Van Winckel (Catholic University of Leuven, Belgium) and Martin Cohen (University of California) The HD 44179 nebula, known as the 'Red Rectangle.' This image, taken with the NASA/ESA Hubble Space Telescope, reveals startling new details of one of the most unusual nebulae known in our galaxy. Catalogued as HD 44179, this nebula is more commonly called the 'Red Rectangle' because of its unique shape and colour as seen with ground-based telescopes. Hubble has revealed a wealth of new features in the Red Rectangle that cannot be seen by ground-based telescopes looking through Earth’s turbulent atmosphere. Details of the Hubble study were published in the April 2004 issue of The Astronomical Journal. HD 44179 Nebula hi-res Size hi-res: 1289 Kb Credits: ESA and Vincent Icke (Leiden University, the Netherlands) Simulating the Red Rectangle The NASA/ESA Hubble Space Telescope has revealed a wealth of new features in the Red Rectangle that cannot be seen with ground-based telescopes looking through the Earth’s turbulent atmosphere. Whereas the origins of many of the features in this dying star still remain hidden or even outright mysterious, some are well explained by theorists like the Dutch scientist Vincent Icke from Leiden University in the Netherlands. In 1981 Vincent Icke and collaborators showed that a spherical gas ejection from a dying star hitting a dust torus would give rise to shocks that can produce cone-like outflows similar to the two cones seen in the Hubble image. Meteorologists produce weather forecasts by advanced calculations of temperatures, pressures, velocities and densities for the air masses in our atmosphere and, to some degree, theorists like Icke are doing exactly the same for objects in space. Whether modelling the weather in the Earth’s atmosphere or the processes in distant gaseous nebulae, scientists calculate the motion of the gas by using a complicated set of

  9. The fast code

    Energy Technology Data Exchange (ETDEWEB)

    Freeman, L.N.; Wilson, R.E. [Oregon State Univ., Dept. of Mechanical Engineering, Corvallis, OR (United States)

    1996-09-01

    The FAST Code which is capable of determining structural loads on a flexible, teetering, horizontal axis wind turbine is described and comparisons of calculated loads with test data are given at two wind speeds for the ESI-80. The FAST Code models a two-bladed HAWT with degrees of freedom for blade bending, teeter, drive train flexibility, yaw, and windwise and crosswind tower motion. The code allows blade dimensions, stiffnesses, and weights to differ and models tower shadow, wind shear, and turbulence. Additionally, dynamic stall is included as are delta-3 and an underslung rotor. Load comparisons are made with ESI-80 test data in the form of power spectral density, rainflow counting, occurrence histograms, and azimuth averaged bin plots. It is concluded that agreement between the FAST Code and test results is good. (au)

  10. A fast friend

    Institute of Scientific and Technical Information of China (English)

    高怡

    2010-01-01

    我们都知道fast food的意思是“快餐”。那fast friend能解释为“快速或速成的朋友”吗?也许你会说:“什么是速成朋友呀?It doesn’t make sense.”没错,交朋友怎么会有速成的呢?原来;fast还有一个意思是“忠实的、牢固的”,所以a fast friend的真正意思是“可靠、忠实的朋友”。

  11. Fast Distributed Gradient Methods

    CERN Document Server

    Jakovetic, Dusan; Moura, Jose M F

    2011-01-01

    The paper proposes new fast distributed optimization gradient methods and proves convergence to the exact solution at rate O(\\log k/k), much faster than existing distributed optimization (sub)gradient methods with convergence O(1/\\sqrt{k}), while incurring practically no additional communication nor computation cost overhead per iteration. We achieve this for convex (with at least one strongly convex,) coercive, three times differentiable and with Lipschitz continuous first derivative (private) cost functions. Our work recovers for distributed optimization similar convergence rate gains obtained by centralized Nesterov gradient and fast iterative shrinkage-thresholding algorithm (FISTA) methods over ordinary centralized gradient methods. We also present a constant step size distributed fast gradient algorithm for composite non-differentiable costs. A simulation illustrates the effectiveness of our distributed methods.

  12. A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH

    DEFF Research Database (Denmark)

    Ramsing, Mette; Becher, Naja Helene; Christensen, Rikke

    A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH......A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH...

  13. Red DirCom

    Directory of Open Access Journals (Sweden)

    Joan Costa

    2007-01-01

    Full Text Available Catorce países congregados de manera activa, a través de una plataforma de encuentro donde se comparten conocimiento y experiencias en la gestión estratégica de la comunicación en las organizaciones. La red reconoce en el DirCom una figura clave del desarrollo corporativo en el nuevo contexto de los negocios, impulsa la exigencia ética a través de la formación y consolida la proyección profesional para posicionar la gestión integral del DirCom en Iberoamérica.

  14. Deep Red (Profondo Rosso)

    CERN Document Server

    Cine Club

    2015-01-01

    Wednesday 29 April 2015 at 20:00 CERN Council Chamber    Deep Red (Profondo Rosso) Directed by Dario Argento (Italy, 1975) 126 minutes A psychic who can read minds picks up the thoughts of a murderer in the audience and soon becomes a victim. An English pianist gets involved in solving the murders, but finds many of his avenues of inquiry cut off by new murders, and he begins to wonder how the murderer can track his movements so closely. Original version Italian; English subtitles

  15. Fast ejendom II

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    Fremstillingen påviser, at lov om forbrugerbeskyttelse ved erhvervelse af fast ejendom mv. lider af en række svagheder og at ankenævnspraksis bevæger sig væk fra retspraksis på en række områder.......Fremstillingen påviser, at lov om forbrugerbeskyttelse ved erhvervelse af fast ejendom mv. lider af en række svagheder og at ankenævnspraksis bevæger sig væk fra retspraksis på en række områder....

  16. Moms og fast ejendom

    DEFF Research Database (Denmark)

    Edlund, Hans Henrik

    1999-01-01

    I artiklen gives et overblik over, hvorledes fast ejendom behandles momsmæssigt. Derfor findes en kort skitsering af reglerne for moms på byggearbejder, afgrænsningen mellem momspligtig og momsfri udlejning, muligheden for frivillig registrering af udlejning samt opgørelse af reguleringsforpligte......I artiklen gives et overblik over, hvorledes fast ejendom behandles momsmæssigt. Derfor findes en kort skitsering af reglerne for moms på byggearbejder, afgrænsningen mellem momspligtig og momsfri udlejning, muligheden for frivillig registrering af udlejning samt opgørelse af...

  17. Fast Josephson vortex

    Energy Technology Data Exchange (ETDEWEB)

    Malishevskii, A.S.; Silin, V.P.; Uryupin, S.A

    2002-12-30

    For the magnetically coupled waveguide and long Josephson junction we gave the analytic description of two separate velocity domains where the free motion of traveling vortex (2{pi}-kink) exists. The role of the mutual influence of waveguide and long Josephson junction is discussed. It is shown the possibility of the fast vortex motion with the velocity much larger than Swihart velocity of Josephson junction and close to the speed of light in the waveguide. The excitation of motion of such fast Josephson vortex is described.

  18. ATLAS fast physics monitoring

    Indian Academy of Sciences (India)

    Karsten Köneke; on behalf of the ATLAS Collaboration

    2012-11-01

    The ATLAS experiment at the Large Hadron Collider is recording data from proton–proton collisions at a centre-of-mass energy of 7 TeV since the spring of 2010. The integrated luminosity has grown nearly exponentially since then and continues to rise fast. The ATLAS Collaboration has set up a framework to automatically process the rapidly growing dataset and produce performance and physics plots for the most interesting analyses. The system is designed to give fast feedback. The histograms are produced within hours of data reconstruction (2–3 days after data taking). Hints of potentially interesting physics signals obtained this way are followed up by physics groups.

  19. Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9.

    Science.gov (United States)

    Kanitkar, Yogendra H; Stedtfeld, Robert D; Steffan, Robert J; Hashsham, Syed A; Cupples, Alison M

    2016-01-08

    Real-time quantitative PCR (qPCR) protocols specific to the reductive dehalogenase (RDase) genes vcrA, bvcA, and tceA are commonly used to quantify Dehalococcoides spp. in groundwater from chlorinated solvent-contaminated sites. In this study, loop-mediated isothermal amplification (LAMP) was developed as an alternative approach for the quantification of these genes. LAMP does not require a real-time thermal cycler (i.e., amplification is isothermal), allowing the method to be performed using less-expensive and potentially field-deployable detection devices. Six LAMP primers were designed for each of three RDase genes (vcrA, bvcA, and tceA) using Primer Explorer V4. The LAMP assays were compared to conventional qPCR approaches using plasmid standards, two commercially available bioaugmentation cultures, KB-1 and SDC-9 (both contain Dehalococcoides species). DNA was extracted over a growth cycle from KB-1 and SDC-9 cultures amended with trichloroethene and vinyl chloride, respectively. All three genes were quantified for KB-1, whereas only vcrA was quantified for SDC-9. A comparison of LAMP and qPCR using standard plasmids indicated that quantification results were similar over a large range of gene concentrations. In addition, the quantitative increase in gene concentrations over one growth cycle of KB-1 and SDC-9 using LAMP was comparable to that of qPCR. The developed LAMP assays for vcrA and tceA genes were validated by comparing quantification on the Gene-Z handheld platform and a real-time thermal cycler using DNA isolated from eight groundwater samples obtained from an SDC-9-bioaugmented site (Tulsa, OK). These assays will be particularly useful at sites subject to bioaugmentation with these two commonly used Dehalococcoides species-containing cultures.

  20. Synthesis and optical characterization of LiKB4O7, Li2B6O10, and LiCsB6O10 glasses

    Energy Technology Data Exchange (ETDEWEB)

    Adamiv, V.; Teslyuk, I.; Dyachok, Ya.; Romanyuk, G.; Krupych, O.; Mys, O.; Martynyuk-Lototska, I.; Burak, Ya.; Vlokh, R.

    2010-10-01

    In the current work we report on the synthesis of LiKB4O7, Li2B6O10, and LiCsB6O10 borate glasses. The results for their piezo-optic, acousto-optic, acoustic, elastic, refractive, optical transmission, and optical resistance properties are also presented. It is shown that some of these glasses represent efficient acousto-optic materials that are transparent down to the vacuum ultraviolet range and highly resistant to laser radiation.

  1. Synthesis and optical characterization of LiKB4O7, Li2B6O10, and LiCsB6O10 glasses.

    Science.gov (United States)

    Adamiv, V; Teslyuk, I; Dyachok, Ya; Romanyuk, G; Krupych, O; Mys, O; Martynyuk-Lototska, I; Burak, Ya; Vlokh, R

    2010-10-01

    In the current work we report on the synthesis of LiKB(4)O(7), Li(2)B(6)O(10), and LiCsB(6)O(10) borate glasses. The results for their piezo-optic, acousto-optic, acoustic, elastic, refractive, optical transmission, and optical resistance properties are also presented. It is shown that some of these glasses represent efficient acousto-optic materials that are transparent down to the vacuum ultraviolet range and highly resistant to laser radiation.

  2. Soggy, a spermatocyte-specific gene, lies 3.8 kb upstream of and antipodal to TEAD-2, a transcription factor expressed at the beginning of mouse development.

    Science.gov (United States)

    Kaneko, K J; DePamphilis, M L

    2000-10-15

    Investigation of the regulatory region of mTEAD-2, a gene expressed at the beginning of mouse pre-implantation development, led to the surprising discovery of another gene only 3.8 kb upstream of mTEAD-2. Here we show that this new gene is a single copy, testis-specific gene called SOGGY: (mSgy) that produces a single, dominant mRNA approximately 1.3 kb in length. It is transcribed in the direction opposite to mTEAD-2, thus placing the regulatory elements of these two genes in close proximity. mSgy contains three methionine codons that could potentially act as translation start sites, but most mSGY protein synthesis in vitro was initiated from the first Met codon to produce a full-length protein, suggesting that mSGY normally consists of 230 amino acids (26.7 kDa). Transcription began at a cluster of nucleotides approximately 150 bp upstream of the first Met codon using a TATA-less promoter contained within the first 0.9 kb upstream. The activity of this promoter was repressed by upstream sequences between -0.9 and -2.5 kb in cells that did not express mSgy, but this repression was relieved in cells that did express mSgy. mSgy mRNA was detected in embryos only after day 15 and in adult tissues only in the developing spermatocytes of seminiferous tubules, suggesting that mSgy is a spermatocyte-specific gene. Since mTEAD-2 and mSgy were not expressed in the same cells, the mSgy/mTEAD-2 locus provides a unique paradigm for differential regulation of gene expression during mammalian development.

  3. Neuropsychological phenotype of a patient with a de novo 970 kb interstitial deletion in the distal 16p11.2 region

    Science.gov (United States)

    Egger, Jos I M; Verhoeven, Willem M A; Verbeeck, Wim; de Leeuw, Nicole

    2014-01-01

    The 16p11.2 microdeletion syndrome is characterized by a wide range of phenotypic expressions and is frequently associated with developmental delay, symptoms from the autism spectrum, epilepsy, congenital anomalies, and obesity. These phenotypes are often related to a proximal 16p11.2 deletion of approximately 600 kb (BP4–BP5) that includes the SH2B1 gene that is reported to be causative for morbid obesity. This more centromeric deletion is most strongly related to autism spectrum susceptibility and is functionally different from the more distal 16p12.2p11.2 region, which includes the so-called atypical 16p11.2 BP2–BP3 deletion (approximately 220 kb) presenting with developmental delay, behavioral problems and mild facial dysmorphisms. Here, an adult male with a long history of maladaptive behaviors is described who was referred for diagnostic assessment of his amotivational features. Extensive neuropsychological examination demonstrated rigid thinking, anxious beliefs, and ideas of reference in the presence of normal intelligence. Microarray analysis demonstrated a de novo 970 kb 16p11.2 BP1–BP4 microdeletion that can be regarded as explanatory for his behavioral profile. It is concluded that microdeletion syndromes are not exclusively related to intellectual disabilities and genetic testing is of putative relevance for the understanding of neuropsychiatric and neuropsychological phenomena. PMID:24707176

  4. Low-Resolution Electromagnetic Tomography (LORETA) of changed Brain Function Provoked by Pro-Dopamine Regulator (KB220z) in one Adult ADHD case

    Science.gov (United States)

    Steinberg, Bruce; Blum, Kenneth; McLaughlin, Thomas; Lubar, Joel; Febo, Marcelo; Braverman, Eric R.; Badgaiyan, Rajendra D

    2016-01-01

    Attention Deficit-Hyperactivity Disorder (ADHD) often continues into adulthood. Recent neuroimaging studies found lowered baseline dopamine tone in the brains of affected individuals that may place them at risk for Substance Use Disorder (SUD). This is an observational case study of the potential for novel management of Adult ADHD with a non-addictive glutaminergic-dopaminergic optimization complex KB200z. Low-resolution electromagnetic tomography (LORETA) was used to evaluate the effects of KB220z on a 72-year-old male with ADHD, at baseline and one hour following administration. The resultant z-scores, averaged across Eyes Closed, Eyes Open and Working Memory conditions, increased for each frequency band, in the anterior, dorsal and posterior cingulate regions, as well as the right dorsolateral prefrontal cortex during Working Memory, with KB220z. These scores are consistent with other human and animal neuroimaging studies that demonstrated increased connectivity volumes in reward circuitry and may offer a new approach to ADHD treatment. However, larger randomized trials to confirm these results are required. PMID:27610420

  5. Low-Resolution Electromagnetic Tomography (LORETA) of changed Brain Function Provoked by Pro-Dopamine Regulator (KB220z) in one Adult ADHD case.

    Science.gov (United States)

    Steinberg, Bruce; Blum, Kenneth; McLaughlin, Thomas; Lubar, Joel; Febo, Marcelo; Braverman, Eric R; Badgaiyan, Rajendra D

    Attention Deficit-Hyperactivity Disorder (ADHD) often continues into adulthood. Recent neuroimaging studies found lowered baseline dopamine tone in the brains of affected individuals that may place them at risk for Substance Use Disorder (SUD). This is an observational case study of the potential for novel management of Adult ADHD with a non-addictive glutaminergic-dopaminergic optimization complex KB200z. Low-resolution electromagnetic tomography (LORETA) was used to evaluate the effects of KB220z on a 72-year-old male with ADHD, at baseline and one hour following administration. The resultant z-scores, averaged across Eyes Closed, Eyes Open and Working Memory conditions, increased for each frequency band, in the anterior, dorsal and posterior cingulate regions, as well as the right dorsolateral prefrontal cortex during Working Memory, with KB220z. These scores are consistent with other human and animal neuroimaging studies that demonstrated increased connectivity volumes in reward circuitry and may offer a new approach to ADHD treatment. However, larger randomized trials to confirm these results are required.

  6. Handel med fast ejendom

    DEFF Research Database (Denmark)

    Edlund, Hans Henrik

    Bogen tilstræber at give et overblik over nogle af de vigtigste generelle problemområder på markedet for ejendomshandel, der jo bliver mere og mere kompliceret. Værket er opdelt i følgende hovedafsnit: Ejendomsbegrebet. Indgåelse af aftale om salg af fast ejendom. Begrænsninger i adgangen til...

  7. Not so fast

    DEFF Research Database (Denmark)

    Marras, Stefano; Noda, Takuji; Steffensen, John Fleng

    2015-01-01

    , it is an open question whether such supposedly very fast swimmers do use high-speed bursts when feeding on evasive prey, in addition to using their bill for slashing prey. Here, we measured the swimming behavior of sailfish by using high-frequency accelerometry and high-speed video observations during predator...

  8. Fast Passenger Tracks Network

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    China’s fast passenger tracks network consists of four parts:express rail- way with speeds between 300km/h and 350 kin/h,passenger rail lines with speeds between 200 km/h and 250 km/h,intercity high-speed railways that run

  9. Integral Fast Reactor concept

    Energy Technology Data Exchange (ETDEWEB)

    Till, C.E.; Chang, Y.I.

    1986-01-01

    The Integral Fast Reactor (IFR) is an innovative LMR concept, being developed at Argonne National Laboratory, that fully exploits the inherent properties of liquid metal cooling and metallic fuel to achieve breakthroughs in economics and inherent safety. This paper describes key features and potential advantages of the IFR concept, technology development status, fuel cycle economics potential, and future development path.

  10. Fast Air Temperature Sensors

    DEFF Research Database (Denmark)

    Hendricks, Elbert

    1998-01-01

    The note documents briefly work done on a newly developed sensor for making fast temperature measurements on the air flow in the intake ports of an SI engine and in the EGR input line. The work reviewed has been carried out in close cooperation with Civ. Ing. Michael Føns, the author (IAU...

  11. Parallel Fast Legendre Transform

    NARCIS (Netherlands)

    Alves de Inda, M.; Bisseling, R.H.; Maslen, D.K.

    2001-01-01

    We discuss a parallel implementation of a fast algorithm for the discrete polynomial Legendre transform We give an introduction to the DriscollHealy algorithm using polynomial arithmetic and present experimental results on the eciency and accuracy of our implementation The algorithms were implemente

  12. Foinaven fast track flowlines

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, L.H.; Mair, J.

    1996-12-31

    The decision by British Petroleum to develop offshore fields west of the Shetlands in water depths exceeding 500 meters within three and a half years of discovery posed a unique submarine pipeline installation challenge. This paper summarizes the salient features of a fast track program to install a diverless subsea pipeline system using rigid reeled pipe technology in an offshore frontier area.

  13. Fast food tips

    Science.gov (United States)

    ... Order smaller servings when you can. Split some fast-food items to reduce calories and fat. Ask for a "doggy bag." You can also leave the extra food on your plate. Your food choices can teach your children how to eat healthy, too. Choosing a variety ...

  14. Fast Fourier Orthogonalization

    NARCIS (Netherlands)

    Ducas, L.; Prest, T.; Abramov, S.A.; Zima, E.V.; Gao, X-S.

    2016-01-01

    The classical fast Fourier transform (FFT) allows to compute in quasi-linear time the product of two polynomials, in the {\\em circular convolution ring} R[x]/(x^d−1) --- a task that naively requires quadratic time. Equivalently, it allows to accelerate matrix-vector products when the matrix is *circ

  15. Tachycardia | Fast Heart Rate

    Science.gov (United States)

    ... SA) node --- the heart's natural pacemaker - sends out electrical signals faster than usual. The heart rate is fast, but the heart beats properly. Causes of sinus tachycardia A rapid heartbeat may be your body's response to common conditions such as: Fever Anxiety ...

  16. MicroRNA-223-3p inhibits the angiogenesis of ischemic cardiac microvascular endothelial cells via affecting RPS6KB1/hif-1a signal pathway.

    Directory of Open Access Journals (Sweden)

    Guo-Hua Dai

    Full Text Available BACKGROUND: MicroRNAs (miRNAs are a recently discovered class of posttranscriptional regulators of gene expression with critical functions in the angiogenesis and cardiovascular diseases; however, the details of miRNAs regulating mechanism of angiogenesis of ischemic cardiac microvascular endothelial cells (CMECs are not yet reported. METHODS AND RESULTS: This study analyzes the changes of the dynamic expression of miRNAs during the process of angiogenesis of ischemic CMECs by applying miRNA chip and real-time PCR for the first time. Compared with normal CMECs, ischemic CMECs have a specific miRNAs expression profile, in which mir-223-3p has the most significant up-regulation, especially during the process of migration and proliferation, while the up-regulation is the most significant during migration, reaching 11.02 times. Rps6kb1 is identified as a potential direct and functional target of mir-223-3p by applying bioinformatic prediction, real-time PCR and Western blot. Pathway analysis report indicates Rps6kb1 regulates the angiogenesis by participating into hif-1a signal pathway. Further analysis reveals that both the gene and protein expression of the downstream molecules VEGF, MAPK, PI3K and Akt of Rps6kb1/hif-1a signal pathway decrease significantly during the process of migration and proliferation in the ischemic CMECs. Therefore, it is confirmed that mir-223-3p inhibits the angiogenesis of CMECs, at least partly, via intervening RPS6KB1/hif-1a signal pathway and affecting the process of migration and proliferation. CONCLUSION: This study elucidates the miRNA regulating law in the angiogenesis of CMECs; mir-223-3p inhibits the process of migration and proliferation of ischemic CMECs probably via affecting RPS6KB1/hif-1a signal pathway, which in turn suppresses the angiogenesis. It is highly possible that mir-223-3p becomes a novel intervention core target in the treatment of angiogenesis of ischemic heart diseases.

  17. Fade to Red

    Science.gov (United States)

    2006-01-01

    [figure removed for brevity, see original site] Infrared Andromeda Galaxy (M31) Poster [figure removed for brevity, see original site] [figure removed for brevity, see original site] Stars Dust This animation shows the Andromeda galaxy, first as seen in visible light by the National Optical Astronomy Observatory, then as seen in infrared by NASA's Spitzer Space Telescope. The visible-light image highlights the galaxy's population of about one trillion stars. The stars are so crammed into its core that this region blazes with bright starlight. In contrast, the false-colored Spitzer view reveals red waves of dust against a more tranquil sea of blue stars. The dust lanes can be seen twirling all the way into the galaxy's center. This dust is warmed by young stars and shines at infrared wavelengths , which are represented in red. The blue color signifies shorter-wavelength infrared light primarily from older stars. The Andromeda galaxy, also known affectionately by astronomers as Messier 31, is located 2.5 million light-years away in the constellation Andromeda. It is the closest major galaxy to the Milky Way, making it the ideal specimen for carefully examining the nature of galaxies. On a clear, dark night, the galaxy can be spotted with the naked eye as a fuzzy blob. Andromeda's entire disk spans about 260,000 light-years, which means that a light beam would take 260,000 years to travel from one end of the galaxy to the other. By comparison, the Milky Way is about 100,000 light-years across. When viewed from Earth, Andromeda occupies a portion of the sky equivalent to seven full moons. Because this galaxy is so large, the infrared images had to be stitched together out of about 3,000 separate Spitzer exposures. The light detected by Spitzer's infrared array camera at 3.6 and 4.5 microns is sensitive mostly to starlight and is shown in blue and green, respectively. The 8-micron light shows warm dust and is shown in red. The contribution from starlight has been

  18. International red meat trade.

    Science.gov (United States)

    Brester, Gary W; Marsh, John M; Plain, Ronald L

    2003-07-01

    The maturation of the US beef and pork markets and increasing consumer demands for convenience, safety, and nutrition suggests that the beef and pork industries must focus on product development and promotion. New marketing arrangements are developing that help coordinate production with consumer demands. The relative high levels of incomes in the United States are likely to increase the demands for branded products rather than increase total per capita consumption. Foreign markets represent the greatest opportunity for increased demand for commodity beef and pork products. Increasing incomes in developing countries will likely allow consumers to increase consumption of animal-source proteins. Real prices of beef and pork have declined substantially because of sagging domestic demand and increasing farm-level production technologies. Increasing US beef and pork exports have obviated some of the price declines. Pork attained a net export position from a quantity perspective in 1995. The United States continues to be a net importer of beef on a quantity basis but is close to becoming a net exporter in terms of value. By-products continue to play a critical role in determining the red meat trade balance and producer prices. The United States, however, must continue to become cost, price, and quality competitive with other suppliers and must secure additional market access if it is to sustain recent trade trends. Several trade tensions remain in the red meat industry. For example, mandated COOL will undoubtedly have domestic and international effects on the beef and pork sectors. Domestically, uncertainty regarding consumer demand responses or quality perceptions regarding product origin, as well as added processor-retailer costs will be nontrivial. How these factors balance out in terms of benefits versus costs to the industry is uncertain. From an international perspective, some beef and pork export suppliers to the United States could view required labeling as a

  19. A Search for Fast Radio Bursts in GALFACTS data

    Science.gov (United States)

    Cohen, Tyler; Salter, Christopher J.; Ghosh, Tapasi

    2016-01-01

    Fast Radio Bursts (FRBs) are transient radio sources whose high dispersion measures suggest they are of extra-galactic origin. They are particularly difficult to detect because, unlike other fast radio transients, they are non-recurring events. At present, 11 such bursts have been detected, 10 by the Parkes Radio Telescope and one by Arecibo Observatory. The G-ALFA Continuum Transit Survey (GALFACTS) is the highest resolution, full-Stokes, radio-continuum survey of the foreground sky. The Arecibo radio telescope is the largest single-aperture telescope in the world, offering the superior point-source sensitivity necessary to detect additional FRBs. GALFACTS utilizes Arecibo's ALFA receiver, an L-band 7-beam feed array, to produce a high-time (1 ms), low-spectral (MHz) resolution (HTLS) data stream between 1225 and 1525 MHz. We used ``Red_Transient", a robust search pipeline developed by A.A. Deshpande, to de-disperse the HTLS data with the intention of detecting FRBs in the ~30% of the total sky surveyed by GALFACTS. Concurrently, the student produced a similar search pipeline to calibrate HTLS data and validate detections by ``Red_Transient". Here, we present the results of initial processing runs on the first several days of GALFACTS observations. Currently, no FRB detections have been found. However, the detection of pulses from the known pulsar J1916+1312 indicates that ``Red_Transient" is capable of detecting fast transient signals present in the data stream.

  20. The expressions of NF-kb and TGFb-1 on odontoblast-like cells of human dental pulp injected with propolis extracts

    Directory of Open Access Journals (Sweden)

    Ira Widjiastuti

    2014-03-01

    Full Text Available Background: Propolis is known to have beneficial effects, namely anti- bacterial, anti-viral, anti-inflammatory, antioxidant, and immunomodulatory. Propolis extracts with anti-inflammatory properties are expected to be useful in treating inflamed pulp tissue with a diagnosis of reversible pulpitis. The inflammation of pulp tissue is caused by bacteria, namely Lactobacillus acidophilus. This research used odontoblast like cells derived from pulp tissue of human third molars. Odontoblast like cells exposed to Lactobacillus achidophilus were used as a model of proinflammatory cytokine signaling. This research examined the effects of propolis extracts on odontoblast like cells exposed to Lactobacillus acidophilus. Purpose: This research was aimed to determine the effectiveness of propolis extracts on the activities of odontoblast-like cells exposed to Lactobacillus acidophillus by measuring the expressions of NFkb and TGF- b1. Methods: First, pulp odontoblast cultures were derived from human dental pulp tissues of impacted third molars removed by using digestion method. Next, odontoblast-like cells exposed to inactive Lactobacillus acidophilus bacteria were given propolis extract. Finally, the activities of odontoblast-like cells were monitored by measuring the expressions of NF-kb and TGFb-1 with immunocytochemistry technique. Results: A decline NF-kb expression and on increase of TGFb-1 expression on odontoblast like cells exposed to inactive Lactobacillus acidophilus. Conclusion: Propolis extracts inhibit the expression of NF-kb, and increase the expression of TGF-b1 in pulp odontoblast-like cells exposed to inactive Lactobacillus acidophillus.Latar belakang: Propolis dilaporkan mempunyai efek menguntungkan yaitu bersifat anti bakteri, anti virus, anti inflamasi, anti oksidan, dan imunomodulator. Ekstrak propolis dengan sifat anti inflamasi diharapkan bermanfaat untuk mengobati jaringan pulpa yang mengalami inflamasi dengan diagnosis pulpitis

  1. Decadal trends in Red Sea maximum surface temperature

    KAUST Repository

    Chaidez, Veronica

    2017-08-09

    Ocean warming is a major consequence of climate change, with the surface of the ocean having warmed by 0.11 °C decade-1 over the last 50 years and is estimated to continue to warm by an additional 0.6 - 2.0 °C before the end of the century1. However, there is considerable variability in the rates experienced by different ocean regions, so understanding regional trends is important to inform on possible stresses for marine organisms, particularly in warm seas where organisms may be already operating in the high end of their thermal tolerance. Although the Red Sea is one of the warmest ecosystems on earth, its historical warming trends and thermal evolution remain largely understudied. We characterized the Red Sea\\'s thermal regimes at the basin scale, with a focus on the spatial distribution and changes over time of sea surface temperature maxima, using remotely sensed sea surface temperature data from 1982 - 2015. The overall rate of warming for the Red Sea is 0.17 ± 0.07 °C decade-1, while the northern Red Sea is warming between 0.40 and 0.45 °C decade-1, all exceeding the global rate. Our findings show that the Red Sea is fast warming, which may in the future challenge its organisms and communities.

  2. The Red and the Yellow

    Institute of Scientific and Technical Information of China (English)

    QiuJianghong

    2004-01-01

    THE China film market is based on the so-called battle of the yellow, green and red.Yellow is American Kodak,green Japanese Fuji color, and red China's Lucky film, local counterweight to the two world-famous foreigners.

  3. Red lantern Streets- Chinese Style

    Institute of Scientific and Technical Information of China (English)

    INESAPLESKACHEUSKAYA

    2005-01-01

    WHEN speaking of ""red lantern streets,""what's the first thing that comes to mind?Sordid alleys, haunted by ladies of the night? While ""red lantern districts"" both in the East and the West have something in common, pleasures here in China are purely gastronomical in nature.

  4. Beyond Fast Mapping

    OpenAIRE

    Carey, Susan

    2010-01-01

    Since the seminal 1957 studies of word learning by Roger Brown, most experimental studies of lexical acquisition have concerned fast mapping: the process through which a new lexical entry is established, and through which representations of the linguistic context of a newly heard word interact with representations of its nonlinguistic context to fix an initial partial meaning. Here I focus on the subsequent extended process through which the adult meaning is approximated. Two factors lead to ...

  5. Fast Radio Bursts

    Indian Academy of Sciences (India)

    Akshaya Rane; Duncan Lorimer

    2017-09-01

    We summarize our current state of knowledge of fast radio bursts (FRBs) which were first discovered a decade ago. Following an introduction to radio transients in general, including pulsars and rotating radio transients, we discuss the discovery of FRBs. We then discuss FRB follow-up observations in the context of repeat bursts before moving on to review propagation effects on FRB signals, FRB progenitor models and an outlook on FRBs as potential cosmological tools.

  6. PHENIX Fast TOF

    Energy Technology Data Exchange (ETDEWEB)

    Soha, Aria [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Chiu, Mickey [Brookhaven National Lab. (BNL), Upton, NY (United States); Mannel, Eric [Brookhaven National Lab. (BNL), Upton, NY (United States); Stoll, Sean [Brookhaven National Lab. (BNL), Upton, NY (United States); Lynch, Don [Brookhaven National Lab. (BNL), Upton, NY (United States); Boose, Steve [Brookhaven National Lab. (BNL), Upton, NY (United States); Northacker, Dave [Brookhaven National Lab. (BNL), Upton, NY (United States); Alfred, Marcus [Howard Univ., Washington, DC (United States); Lindesay, James [Howard Univ., Washington, DC (United States); Chujo, Tatsuya [Univ. of Tsukuba (Japan); Inaba, Motoi [Univ. of Tsukuba (Japan); Nonaka, Toshihiro [Univ. of Tsukuba (Japan); Sato, Wataru [Univ. of Tsukuba (Japan); Sakatani, Ikumi [Univ. of Tsukuba (Japan); Hirano, Masahiro [Univ. of Tsukuba (Japan); Choi, Ihnjea [Univ. of Illinois, Urbana-Champaign, IL (United States)

    2014-01-15

    This is a technical scope of work (TSW) between the Fermi National Accelerator Laboratory (Fermilab) and the experimenters of PHENIX Fast TOF group who have committed to participate in beam tests to be carried out during the FY2014 Fermilab Test Beam Facility program. The goals for this test beam experiment are to verify the timing performance of the two types of time-of-flight detector prototypes.

  7. Fast Air Temperature Sensors

    DEFF Research Database (Denmark)

    Hendricks, Elbert

    1998-01-01

    The note documents briefly work done on a newly developed sensor for making fast temperature measurements on the air flow in the intake ports of an SI engine and in the EGR input line. The work reviewed has been carried out in close cooperation with Civ. Ing. Michael Føns, the author (IAU......) and Spencer C. Sorenson (ET). The theory which decribes in detail the overall dynamic chracteristics of the sensor was developed at IAU, DTU....

  8. Vancomycin induced Red Man Syndrome

    Directory of Open Access Journals (Sweden)

    Drisyamol K.A

    2016-04-01

    Full Text Available Vancomycin is a glycoprotein antibiotic that has been associated with an anaphylactoid reaction termed the Red-man syndrome. It usually consists of erythema, flushing and pruritis of the face and upper torso and occasionally progresses to include dyspnoea, chest pain and hypotension. Red man syndrome (RMS is also known as “red neck syndrome. Discontinuation of the vancomycin infusion and administration of diphenhydramine can abort most of the reactions. Slow intravenous administration of vancomycin should minimize the risk of infusion-related adverse effects. Antibiotics such as ciprofloxacin, amphotericin B, rifampcin and teicoplanin can potentially cause red man syndrome. The effects of red man syndrome can be relieved by antihistamines.

  9. Polysaccharides of the red algae.

    Science.gov (United States)

    Usov, Anatolii I

    2011-01-01

    Red algae (Rhodophyta) are known as the source of unique sulfated galactans, such as agar, agarose, and carrageenans. The wide practical uses of these polysaccharides are based on their ability to form strong gels in aqueous solutions. Gelling polysaccharides usually have molecules built up of repeating disaccharide units with a regular distribution of sulfate groups, but most of the red algal species contain more complex galactans devoid of gelling ability because of various deviations from the regular structure. Moreover, several red algae may contain sulfated mannans or neutral xylans instead of sulfated galactans as the main structural polysaccharides. This chapter is devoted to a description of the structural diversity of polysaccharides found in the red algae, with special emphasis on the methods of structural analysis of sulfated galactans. In addition to the structural information, some data on the possible use of red algal polysaccharides as biologically active polymers or as taxonomic markers are briefly discussed. Copyright © 2011 Elsevier Inc. All rights reserved.

  10. Elevated red blood cell distribution width is associated with intrahepatic cholestasis of pregnancy.

    Science.gov (United States)

    Vural Yilmaz, Zehra; Gencosmanoglu Turkmen, Gulenay; Daglar, Korkut; Yılmaz, Elif; Kara, Ozgur; Uygur, Dilek

    2017-01-01

    Intrahepatic cholestasis of pregnancy is the most common pregnancy specific liver disease and related with adverse maternal and perinatal outcome. Red blood cell distribution width, an anisocytosis marker in a complete blood count, has been used as an inflammation marker in various diseases. However the association of red blood cell distribution width with intrahepatic cholestasis of pregnancy is unknown. We aimed to evaluate the relationship between red blood cell distribution width and intrahepatic cholestasis of pregnancy. Ninety pregnant women with intrahepatic cholestasis of pregnancy and ninety healthy pregnant women were included in the study. Their clinical and laboratory characteristics including red blood cell distribution width, liver function tests, fasting and postprandial bile acid concentrations were analyzed. Serum red blood cell distribution width cell levels were significantly higher in pregnants with intrahepatic cholestasis of pregnancy than healthy pregnants. We also demonstrated that red blood cell distribution Width levels were higher in severe disease than mild disease and was significantly correlated with fasting and postprandial bile acid concentration in intrahepatic cholestasis of pregnancy group. Our study showed that red blood cell distribution width, an easy and inexpensive marker; were associated with intrahepatic cholestasis of pregnancy and can be used as a diagnostic and prognostic marker in intrahepatic cholestasis of pregnancy.

  11. A relationship between red tide outbreaks and urban development along the coasts of Guangdong Province

    Institute of Scientific and Technical Information of China (English)

    LIUXiaonan; WANGWei

    2004-01-01

    Red tides are one of the main coastal catastrophic events in Guangdong Province of southern China. The comparison between the number of red tide events and the development indexes of cities along the coasts of the province shows that the regional differences in red tide outbreaks has close relations with the coastal urban developments. The cause for an initiation of red tide blooms may be natural factors, while wastewater caused by the fast development of population, industry and aquiculture of the coastal cities enhanced the blooms. It may explain why the two periods of frequent outbreaks of the red tides over the last two decades matched the urban developments in the coastal areas of Guangdong not only spatially but also temporally. The red tides in the first period were restricted only to the coasts of middle Guangdong, where urbanization process was at a higher speed than the other coastal areas of the province. In the second period, fast development of the coastal cities in eastern Guangdong led to an increase in the occurrence of red tides in local sea areas of the same coasts.

  12. ADT fast losses MD

    CERN Document Server

    Priebe, A; Dehning, B; Redaelli, S; Salvachua Ferrando, BM; Sapinski, M; Solfaroli Camillocci, M; Valuch, D

    2013-01-01

    The fast beam losses in the order of 1 ms are expected to be a potential major luminosity limitation for higher beam energies after the LHC long shutdown (LS1). Therefore a Quench Test is planned in the winter 2013 to estimate the quench limit in this timescale and revise the current models. This experiment was devoted to determination the LHC Transverse Damper (ADT) as a system for fast losses induction. A non-standard operation of the ADT was used to develop the beam oscillation instead of suppressing them. The sign flip method had allowed us to create the fast losses within several LHC turns at 450 GeV during the previous test (26th March 2012). Thus, the ADT could be potentially used for the studies of the UFO ("Unidentied Falling Object") impact on the cold magnets. Verification of the system capability and investigations of the disturbed beam properties were the main objectives of this MD. During the experiment, the pilot bunches of proton beam were excited independently in the horizontal and vertical ...

  13. Fast Light Optical Gyroscopes

    Science.gov (United States)

    Smith, David D.

    2015-01-01

    Next-generation space missions are currently constrained by existing spacecraft navigation systems which are not fully autonomous. These systems suffer from accumulated dead-reckoning errors and must therefore rely on periodic corrections provided by supplementary technologies that depend on line-of-sight signals from Earth, satellites, or other celestial bodies for absolute attitude and position determination, which can be spoofed, incorrectly identified, occluded, obscured, attenuated, or insufficiently available. These dead-reckoning errors originate in the ring laser gyros themselves, which constitute inertial measurement units. Increasing the time for standalone spacecraft navigation therefore requires fundamental improvements in gyroscope technologies. One promising solution to enhance gyro sensitivity is to place an anomalous dispersion or fast light material inside the gyro cavity. The fast light essentially provides a positive feedback to the gyro response, resulting in a larger measured beat frequency for a given rotation rate as shown in figure 1. Game Changing Development has been investing in this idea through the Fast Light Optical Gyros (FLOG) project, a collaborative effort which began in FY 2013 between NASA Marshall Space Flight Center (MSFC), the U.S. Army Aviation and Missile Research, Development, and Engineering Center (AMRDEC), and Northwestern University. MSFC and AMRDEC are working on the development of a passive FLOG (PFLOG), while Northwestern is developing an active FLOG (AFLOG). The project has demonstrated new benchmarks in the state of the art for scale factor sensitivity enhancement. Recent results show cavity scale factor enhancements of approx.100 for passive cavities.

  14. Metabolic Effects of Intermittent Fasting.

    Science.gov (United States)

    Patterson, Ruth E; Sears, Dorothy D

    2017-08-21

    The objective of this review is to provide an overview of intermittent fasting regimens, summarize the evidence on the health benefits of intermittent fasting, and discuss physiological mechanisms by which intermittent fasting might lead to improved health outcomes. A MEDLINE search was performed using PubMed and the terms "intermittent fasting," "fasting," "time-restricted feeding," and "food timing." Modified fasting regimens appear to promote weight loss and may improve metabolic health. Several lines of evidence also support the hypothesis that eating patterns that reduce or eliminate nighttime eating and prolong nightly fasting intervals may result in sustained improvements in human health. Intermittent fasting regimens are hypothesized to influence metabolic regulation via effects on (a) circadian biology, (b) the gut microbiome, and (c) modifiable lifestyle behaviors, such as sleep. If proven to be efficacious, these eating regimens offer promising nonpharmacological approaches to improving health at the population level, with multiple public health benefits.

  15. Fast Screening of Antibacterial Compounds from Fusaria

    Directory of Open Access Journals (Sweden)

    Teis Esben Sondergaard

    2016-11-01

    Full Text Available Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus Fusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. The system was then used in a bio-guided screen of extracts from 14 different Fusarium species, which had been fractionated by HPLC. In this screen, fractions containing the red pigments aurofusarin and bikaverin showed effects against strains of Lactobacillus and Bifidobacterium. The IC50 for aurofusarin against Lactobacillus acidophilus was 8 µM, and against Bifidobacterium breve it was 64 µM. Aurofusarin only showed an effect on probiotic bacteria, leading to the speculation that only health-promoting bacteria with a positive effect in the gut system are affected.

  16. Neighborhood fast food availability and fast food consumption.

    Science.gov (United States)

    Oexle, Nathalie; Barnes, Timothy L; Blake, Christine E; Bell, Bethany A; Liese, Angela D

    2015-09-01

    Recent nutritional and public health research has focused on how the availability of various types of food in a person's immediate area or neighborhood influences his or her food choices and eating habits. It has been theorized that people living in areas with a wealth of unhealthy fast-food options may show higher levels of fast-food consumption, a factor that often coincides with being overweight or obese. However, measuring food availability in a particular area is difficult to achieve consistently: there may be differences in the strict physical locations of food options as compared to how individuals perceive their personal food availability, and various studies may use either one or both of these measures. The aim of this study was to evaluate the association between weekly fast-food consumption and both a person's perceived availability of fast-food and an objective measure of fast-food presence - Geographic Information Systems (GIS) - within that person's neighborhood. A randomly selected population-based sample of eight counties in South Carolina was used to conduct a cross-sectional telephone survey assessing self-report fast-food consumption and perceived availability of fast food. GIS was used to determine the actual number of fast-food outlets within each participant's neighborhood. Using multinomial logistic regression analyses, we found that neither perceived availability nor GIS-based presence of fast-food was significantly associated with weekly fast-food consumption. Our findings indicate that availability might not be the dominant factor influencing fast-food consumption. We recommend using subjective availability measures and considering individual characteristics that could influence both perceived availability of fast food and its impact on fast-food consumption. If replicated, our findings suggest that interventions aimed at reducing fast-food consumption by limiting neighborhood fast-food availability might not be completely effective.

  17. Charge exchange spectroscopy as a fast ion diagnostic on TEXTORa)

    Science.gov (United States)

    Delabie, E.; Jaspers, R. J. E.; von Hellermann, M. G.; Nielsen, S. K.; Marchuk, O.

    2008-10-01

    An upgraded charge exchange spectroscopy diagnostic has been taken into operation at the TEXTOR tokamak. The angles of the viewing lines with the toroidal magnetic field are close to the pitch angles at birth of fast ions injected by one of the neutral beam injectors. Using another neutral beam for active spectroscopy, injected counter the direction in which fast ions injected by the first beam are circulating, we can simultaneously measure a fast ion tail on the blue wing of the Dα spectrum while the beam emission spectrum is Doppler shifted to the red wing. An analysis combining the two parts of the spectrum offers possibilities to improve the accuracy of the absolute (fast) ion density profiles. Fast beam modulation or passive viewing lines cannot be used for background subtraction on this diagnostic setup and therefore the background has to be modeled and fitted to the data together with a spectral model for the slowing down feature. The analysis of the fast ion Dα spectrum obtained with the new diagnostic is discussed.

  18. Serum Lipid Profile: Fasting or Non-fasting?

    OpenAIRE

    Nigam, P. K.

    2010-01-01

    Serum lipid profile has now become almost a routine test. It is usually done in fasting state due to certain limitations in non-fasting serum sample. In the recent past efforts have been made to simplify blood sampling by replacing fasting lipid profile with non-fasting lipid profile. However, fasting specimen is preferred if cardiovascular risk assessment is based on total cholesterol, LDL cholesterol or non-HDL cholesterol. A lot has yet to be done in this area. Till then we have to believe...

  19. Serum Lipid Profile: Fasting or Non-fasting?

    OpenAIRE

    Nigam, P. K.

    2010-01-01

    Serum lipid profile has now become almost a routine test. It is usually done in fasting state due to certain limitations in non-fasting serum sample. In the recent past efforts have been made to simplify blood sampling by replacing fasting lipid profile with non-fasting lipid profile. However, fasting specimen is preferred if cardiovascular risk assessment is based on total cholesterol, LDL cholesterol or non-HDL cholesterol. A lot has yet to be done in this area. Till then we have to believe...

  20. Role of fast ignitor in fast-shock ignition concept

    Directory of Open Access Journals (Sweden)

    S. A. Ghasemi

    2014-03-01

    Full Text Available This paper deals with the role of fast ignitor in fast-shock ignition (FSI concept. The semi-analytical model indicates that the FSI target gain is a function of fast ignitor laser wavelength. If the energy of fast ignitor driver is and the laser wavelength is less than 0.53 micron, then with a fuel mass about 2 mg the FSI has a considerable advantage over pure shock ignition and the figure of merit is better than 1.2. When the wavelength of fast ignitor becomes shorter, the approaches , and for wavelengths shorter than 0.25 micron no additional is advantage is obtained.

  1. Hispanics in Fast Food Jobs.

    Science.gov (United States)

    Charner, Ivan; Fraser, Bryna Shore

    A study examined the employment of Hispanics in the fast-food industry. Data were obtained from a national survey of employees at 279 fast-food restaurants from seven companies in which 194 (4.2 percent) of the 4,660 respondents reported being Hispanic. Compared with the total sample, Hispanic fast-food employees were slightly less likely to be…

  2. Putative dopamine agonist (KB220Z) attenuates lucid nightmares in PTSD patients: role of enhanced brain reward functional connectivity and homeostasis redeeming joy.

    Science.gov (United States)

    McLaughlin, Thomas; Blum, Kenneth; Oscar-Berman, Marlene; Febo, Marcelo; Agan, Gozde; Fratantonio, James L; Simpatico, Thomas; Gold, Mark S

    2015-06-01

    Lucid dreams are frequently pleasant and training techniques have been developed to teach dreamers to induce them. In addition, the induction of lucid dreams has also been used as a way to ameliorate nightmares. On the other hand, lucid dreams may be associated with psychiatric conditions, including Post-Traumatic Stress Disorder (PTSD) and Reward Deficiency Syndrome-associated diagnoses. In the latter conditions, lucid dreams can assume an unpleasant and frequently terrifying character. We present two cases of dramatic alleviation of terrifying lucid dreams in patients with PTSD. In the first case study, a 51-year-old, obese woman, diagnosed with PTSD and depression, had attempted suicide and experienced terrifying lucid nightmares linked to sexual/physical abuse from early childhood by family members including her alcoholic father. Her vivid "bad dreams" remained refractory in spite of 6 months of treatment with Dialectical Behavioral Therapy (DBT) and standard pharmaceutical agents which included prazosin, clonidie and Adderall. The second 39-year-old PTSD woman patient had also suffered from lucid nightmares. The medication visit notes reveal changes in the frequency, intensity and nature of these dreams after the complex putative dopamine agonist KB220Z was added to the first patient's regimen. The patient reported her first experience of an extended period of happy dreams. The second PTSD patient, who had suffered from lucid nightmares, was administered KB220Z to attenuate methadone withdrawal symptoms and incidentally reported dreams full of happiness and laughter. These cases are discussed with reference to the known effects of KB220Z including enhanced dopamine homeostasis and functional connectivity of brain reward circuitry in rodents and humans. Their understanding awaits intensive investigation involving large-population, double-blinded studies.

  3. The 253-kb inversion and deep intronic mutations in UNC13D are present in North American patients with familial hemophagocytic lymphohistiocytosis 3.

    Science.gov (United States)

    Qian, Yaping; Johnson, Judith A; Connor, Jessica A; Valencia, C Alexander; Barasa, Nathaniel; Schubert, Jeffery; Husami, Ammar; Kissell, Diane; Zhang, Ge; Weirauch, Matthew T; Filipovich, Alexandra H; Zhang, Kejian

    2014-06-01

    The mutations in UNC13D are responsible for familial hemophagocytic lymphohistiocytosis (FHL) type 3. A 253-kb inversion and two deep intronic mutations, c.118-308C > T and c.118-307G > A, in UNC13D were recently reported in European and Asian FHL3 patients. We sought to determine the prevalence of these three non-coding mutations in North American FHL patients and evaluate the significance of examining these new mutations in genetic testing. We performed DNA sequencing of UNC13D and targeted analysis of these three mutations in 1,709 North American patients with a suspected clinical diagnosis of hemophagocytic lymphohistiocytosis (HLH). The 253-kb inversion, intronic mutations c.118-308C > T and c.118-307G > A were found in 11, 15, and 4 patients, respectively, in which the genetic basis (bi-allelic mutations) explained 25 additional patients. Taken together with previously diagnosed FHL3 patients in our HLH patient registry, these three non-coding mutations were found in 31.6% (25/79) of the FHL3 patients. The 253-kb inversion, c.118-308C > T and c.118-307G > A accounted for 7.0%, 8.9%, and 1.3% of mutant alleles, respectively. Significantly, eight novel mutations in UNC13D are being reported in this study. To further evaluate the expression level of the newly reported intronic mutation c.118-307G > A, reverse transcription PCR and Western blot analysis revealed a significant reduction of both RNA and protein levels suggesting that the c.118-307G > A mutation affects transcription. These specified non-coding mutations were found in a significant number of North American patients and inclusion of them in mutation analysis will improve the molecular diagnosis of FHL3. © 2014 Wiley Periodicals, Inc.

  4. Subgenome chromosome walking in wheat: A 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.)

    Science.gov (United States)

    Stein, Nils; Feuillet, Catherine; Wicker, Thomas; Schlagenhauf, Edith; Keller, Beat

    2000-01-01

    For many agronomically important plant genes, only their position on a genetic map is known. In the absence of an efficient transposon tagging system, such genes have to be isolated by map-based cloning. In bread wheat Triticum aestivum, the genome is hexaploid, has a size of 1.6 × 1010 bp, and contains more than 80% of repetitive sequences. So far, this genome complexity has not allowed chromosome walking and positional cloning. Here, we demonstrate that chromosome walking using bacterial artificial chromosome (BAC) clones is possible in the diploid wheat Triticum monococcum (Am genome). BAC end sequences were mostly repetitive and could not be used for the first walking step. New probes corresponding to rare low-copy sequences were efficiently identified by low-pass DNA sequencing of the BACs. Two walking steps resulted in a physical contig of 450 kb on chromosome 1AmS. Genetic mapping of the probes derived from the BAC contig demonstrated perfect colinearity between the physical map of T. monococcum and the genetic map of bread wheat on chromosome 1AS. The contig genetically spans the Lr10 leaf rust disease resistance locus in bread wheat, with 0.13 centimorgans corresponding to 300 kb between the closest flanking markers. Comparison of the genetic to physical distances has shown large variations within 350 kb of the contig. The physical contig can now be used for the isolation of the orthologous regions in bread wheat. Thus, subgenome chromosome walking in wheat can produce large physical contigs and saturate genomic regions to support positional cloning. PMID:11078510

  5. Isolation and identification of antibacterial neo-compounds from the red ants of ChangBai Mountain, Tetramorium sp.

    Science.gov (United States)

    Song, Zheng-Wei; Liu, Pu; Yin, Wei-Ping; Jiang, Ya-Ling; Ren, Yun-Lai

    2012-03-15

    Three novel coumarin compounds along with two known amide alkaloids were isolated from a methanol extract of the red ants of ChangBai Mountain, Tetramorium sp. Their structures were identified on the basis of IR, 2D NMR ((1)H-(1)H COSY, HSQC, HMBC and NOESY) and HRESIMS analysis. Antibacterial activity of all the compounds was evaluated using KB paper diffusion through measurement of inhibiting zone. It was found that four of all the compounds exhibited significant inhibitory activity against Gram-positive bacteria Bacillus subtilis with MIC values of 25 μg/ml (compounds 1-3) and 15 μg/ml (compound 4).

  6. Red blood cells, multiple sickle cells (image)

    Science.gov (United States)

    Sickle cell anemia is an inherited disorder in which abnormal hemoglobin (the red pigment inside red blood cells) is produced. The abnormal hemoglobin causes red blood cells to assume a sickle shape, like the ones seen in this photomicrograph.

  7. KB220Z™ a Pro-Dopamine Regulator Associated with the Protracted, Alleviation of Terrifying Lucid Dreams. Can We Infer Neuroplasticity-induced Changes in the Reward Circuit?

    Science.gov (United States)

    McLaughlin, Thomas; Febo, Marcelo; Badgaiyan, Rajendra D; Barh, Debmalya; Dushaj, Kristina; Braverman, Eric R; Li, Mona; Madigan, Margaret A; Blum, Kenneth

    2016-01-01

    Recent reports by our laboratory have indicated that lucid dreams may be linked to psychiatric conditions, including Attention Deficit Hyperactivity Disorder (ADHD) and other Reward Deficiency Syndrome-related diagnoses. In the latter case, it has been our observation that such lucid dreams can be unpleasant and frequently terrifying. We present four cases of a dramatic and persistent alleviation of terrifying, lucid dreams in patients diagnosed with ADHD/PTSD and/or opiate/opioid addiction. The amelioration of such dreams could well be permanent, since the patients had stopped taking the nutraceutical for between 10 to 12 months, without their recollection or recurrence. In the first case, the patient is a 47-year-old, married male who required continued Buprenorphine/ Naloxone (Suboxone) treatment. The second case involved a 32-year-old female with the sole diagnosis of ADHD. The third case involves a 38-year-old male who carried the diagnoses of Substance Use Dependence and ADHD. The fourth case involved a 50-year-old female with the diagnoses of Alcohol Abuse, ADHD and Posttraumatic Stress Disorder. In order to attempt to understand the possibility of neuroplasticity, we evaluated the effect of KB220Z in non-opioid-addicted rats utilizing functional Magnetic Resonance Imaging methodology. While we cannot make a definitive claim because rat brain functional connectivity may not be exactly the same as humans, it does provide some interesting clues. We did find following seeding of the dorsal hippocampus, enhanced connectivity volume across several Regions of Interest (ROI), with the exception of the pre- frontal cortex. Interestingly, the latter region is only infrequently activated in lucid human dreaming, when the dreamer reports that he/she had the thought that they were dreaming during the lucid dream. The four patients initially reported a gradual but, then, complete amelioration of their long-term, terrifying, lucid dreams, while taking KB220Z. The

  8. EFECTO DE LA SOBRECARGA DE HIERRO SOBRE LA ACTIVACION DE INF-KB EN CELULAS DE ENDOMETRIO HUMANO COMO POSIBLE MECANISMO ASOCIADO AL A PATOGENESIS DE LA ENDOMETRIOSIS

    OpenAIRE

    ALVARADO DIAZ, CARLOS PATRICIO

    2014-01-01

    La endometriosis es una enfermedad en la cual el tejido endometrial crece fuera de la cavidad uterina. El establecimiento de lesiones endometriósicas peritoneales se originaría a partir de células endometriales refluidas a través de las trompas uterinas durante la menstruación. Asociado al desarrollo de la enfermedad se encuentra un ambiente intraperitoneal pro-inflamatorio. Se ha visto que el factor de transcripción pro-inflamatorio nucelar factr-kappaB (NF-KB), en particular el dímero de NF...

  9. Sequence analysis of a 10 kb DNA fragment from yeast chromosome VII reveals a novel member of the DnaJ family.

    Science.gov (United States)

    Rodriguez-Belmonte, E; Rodriguez-Torres, A M; Tizon, B; Cadahia, J L; Gonzalez-Siso, I; Ramil, E; Becerra, M; Gonzalez-Dominguez, M; Cerdan, E

    1996-02-01

    We report the sequence analysis of a 10 kb DNA fragment of Saccharomyces cerevisiae chromosome VII. This sequence contains four complete open reading frames (ORFs) of greater than 100 amino acids. There are also two incomplete ORFs flanking the extremes: one of these, G2868, is the 5' part of the SCS3 gene (Hosaka et al., 1994). ORFs G2853 and G2856 correspond to the genes CEG1, coding for the alfa subunit of the mRNA guanylyl transferase and a 3' gene of unknown function previously sequenced (Shibagaki et al., 1992). G2864 is identical to SOH1 also reported (Fan and Klein, 1994).

  10. KB220Z™ a Pro-Dopamine Regulator Associated with the Protracted, Alleviation of Terrifying Lucid Dreams. Can We Infer Neuroplasticity-induced Changes in the Reward Circuit?

    Science.gov (United States)

    McLaughlin, Thomas; Febo, Marcelo; Badgaiyan, Rajendra D.; Barh, Debmalya; Dushaj, Kristina; Braverman, Eric R.; Li, Mona; Madigan, Margaret A.; Blum, Kenneth

    2017-01-01

    Background Recent reports by our laboratory have indicated that lucid dreams may be linked to psychiatric conditions, including Attention Deficit Hyperactivity Disorder (ADHD) and other Reward Deficiency Syndrome-related diagnoses. In the latter case, it has been our observation that such lucid dreams can be unpleasant and frequently terrifying. Case presentations We present four cases of a dramatic and persistent alleviation of terrifying, lucid dreams in patients diagnosed with ADHD/PTSD and/or opiate/opioid addiction. The amelioration of such dreams could well be permanent, since the patients had stopped taking the nutraceutical for between 10 to 12 months, without their recollection or recurrence. In the first case, the patient is a 47-year-old, married male who required continued Buprenorphine/ Naloxone (Suboxone) treatment. The second case involved a 32-year-old female with the sole diagnosis of ADHD. The third case involves a 38-year-old male who carried the diagnoses of Substance Use Dependence and ADHD. The fourth case involved a 50-year-old female with the diagnoses of Alcohol Abuse, ADHD and Posttraumatic Stress Disorder. Results In order to attempt to understand the possibility of neuroplasticity, we evaluated the effect of KB220Z in non-opioid-addicted rats utilizing functional Magnetic Resonance Imaging methodology. While we cannot make a definitive claim because rat brain functional connectivity may not be exactly the same as humans, it does provide some interesting clues. We did find following seeding of the dorsal hippocampus, enhanced connectivity volume across several Regions of Interest (ROI), with the exception of the pre- frontal cortex. Interestingly, the latter region is only infrequently activated in lucid human dreaming, when the dreamer reports that he/she had the thought that they were dreaming during the lucid dream. Conclusions The four patients initially reported a gradual but, then, complete amelioration of their long

  11. Red Clump Stars

    Science.gov (United States)

    Girardi, Léo

    2016-09-01

    Low-mass stars in their core-helium-burning stage define the sharpest feature present in the color-magnitude diagrams of nearby galaxy systems: the red clump (RC). This feature has given rise to a series of methods aimed at measuring the distributions of stellar distances and extinctions, especially in the Magellanic Clouds and Milky Way Bulge. Because the RC is easily recognizable within the data of large spectroscopic and asteroseismic surveys, it is a useful probe of stellar densities, kinematics, and chemical abundances across the Milky Way disk; it can be applied up to larger distances than that allowed by dwarfs; and it has better accuracy than is possible with other kinds of giants. Here, we discuss the reasons for the RC narrowness in several sets of observational data, its fine structure, and the presence of systematic changes in the RC properties as regards age, metallicity, and the observed passband. These factors set the limits on the validity and accuracy of several RC methods defined in the literature.

  12. Red Yeast Rice

    Science.gov (United States)

    Nguyen, Thu; Karl, Mitchell; Santini, Antonello

    2017-01-01

    Red yeast rice (RYR), produced by the fermentation of the Monascus purpureus mold, has been used for a long time in Asian cuisine and traditional medicine. It consists of multiple bioactive substances, including monacolins, which potentially can be used as a nutraceutical. Monacolin K, which is chemically identical to lovastatin, has been recognized as responsible for the cholesterol-reducing effect of this compound. While the European Food Safety Authority maintains that the use of monacolin K from RYR preparations of at least 10 mg can produce a normal blood cholesterol level, the United States Food and Drug Administration considers monacolin K, due to its similarity with lovastatin, an unapproved drug, and therefore marketing of products that label the monacolin content is prohibited. This mini-review summarizes the benefit of RYR in hyperlipidemia, maintains RYR use as a food, and addresses the importance of regulation regarding RYR and the need for clinical data and clear label information for consumers with reference to a toxin-free, non-augmented, standardized amount of monacolins. PMID:28257063

  13. Red Yeast Rice

    Directory of Open Access Journals (Sweden)

    Thu Nguyen

    2017-03-01

    Full Text Available Red yeast rice (RYR, produced by the fermentation of the Monascus purpureus mold, has been used for a long time in Asian cuisine and traditional medicine. It consists of multiple bioactive substances, including monacolins, which potentially can be used as a nutraceutical. Monacolin K, which is chemically identical to lovastatin, has been recognized as responsible for the cholesterolreducing effect of this compound. While the European Food Safety Authority maintains that the use of monacolin K from RYR preparations of at least 10 mg can produce a normal blood cholesterol level, the United States Food and Drug Administration considers monacolin K, due to its similarity with lovastatin, an unapproved drug, and therefore marketing of products that label the monacolin content is prohibited. This mini-review summarizes the benefit of RYR in hyperlipidemia, maintains RYR use as a food, and addresses the importance of regulation regarding RYR and the need for clinical data and clear label information for consumers with reference to a toxin-free, nonaugmented, standardized amount of monacolins.

  14. Effect of space allowance during transport and fasting or non-fasting during lairage on welfare indicators in Merino lambs

    Energy Technology Data Exchange (ETDEWEB)

    Cozar, A.; Rodriguez, A.I.; Garijo, P.; Calvo, L.; Vergara, H.

    2016-11-01

    A total of 72 male lambs of Merina breed were sampled in a 3×2 factorial design, testing three different space allowances treatment (SA) during transport [0.16 m2/animal (SAL; n=24); 0.20 m2/animal (SAM; n=24) and 0.30 m2/animal (SAH; n=24)] and two lairage treatments (TL) during 18 h previous slaughter [fasting (FAST; n=36) vs feeding (FEED; n=36)] on welfare physiological indicators. After transport, glucose and lactate dehydrogenase (LDH) were highest in SAM group and lowest in SAH one (p<0.05). SAL showed intermediate values for both parameters. SA did not affect the rest of the blood parameters studied. TL-FAST treatment decreased glucose values (p<0.001) while increased LDH (p<0.001). Fasting caused an increase (p<0.05) of Red Blood Cell Count values in SAM group. Feed deprivation did not affect cortisol or adrenaline values. Noradrenaline value was higher (p<0.001) in TL-FAST groups than in TL-FEED. In conclusion, under the conditions of this study, a range of space allowance during transport between 0.16 and 0.30 m2/lamb could be recommended without showing major changes on welfare physiological indicators; and feeding could be more appropriate than fasting during lairage. (Author)

  15. Romantic red: red enhances men's attraction to women.

    Science.gov (United States)

    Elliot, Andrew J; Niesta, Daniela

    2008-11-01

    In many nonhuman primates, the color red enhances males' attraction to females. In 5 experiments, the authors demonstrate a parallel effect in humans: Red, relative to other achromatic and chromatic colors, leads men to view women as more attractive and more sexually desirable. Men seem unaware of this red effect, and red does not influence women's perceptions of the attractiveness of other women, nor men's perceptions of women's overall likeability, kindness, or intelligence. The findings have clear practical implications for men and women in the mating game and, perhaps, for fashion consultants, product designers, and marketers. Furthermore, the findings document the value of extending research on signal coloration to humans and of considering color as something of a common language, both within and across species.

  16. Pediatric red cell disorders and pure red cell aplasia.

    Science.gov (United States)

    Perkins, Sherrie L

    2004-12-01

    Anemia in children may arise from a wide variety of pathogenetic mechanisms that include congenital and acquired disorders. Often the diagnostic considerations include disorders that are not seen commonly in adults and lifelong disorders that arise in children and persist throughout life. Consideration of diverse causes of anemia such as red cell membrane disorders, red cell enzymopathies, congenital dyserythropoietic anemias, congenital sideroblastic anemias, and hereditary pure red cell aplasia (Diamond-Blackfan anemia), as well as infectious causes such as parvovirus B19 infection, often is required when diagnosing anemia in an infant or young child. Knowledge of these entities that are important causes of anemia in the pediatric population, including clinical manifestations and laboratory workup, will aid in recognition of the specific disease entities and effective workup of pediatric red cell disorders.

  17. Red meat and colorectal cancer

    Directory of Open Access Journals (Sweden)

    Nuri Faruk Aykan

    2015-12-01

    Full Text Available Colorectal cancer (CRC is the third most common cancer in men and the second in women worldwide. More than half of cases occur in more developed countries. The consumption of red meat (beef, pork, lamb, veal, mutton is high in developed countries and accumulated evidence until today demonstrated a convincing association between the intake of red meat and especially processed meat and CRC risk. In this review, meta-analyses of prospective epidemiological studies addressed to this association, observed link of some subtypes of red meat with CRC risk, potential carcinogenic compounds, their mechanisms and actual recommendations of international guidelines are presented.

  18. 14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and regulation upon fasting and by insulin.

    Science.gov (United States)

    Wing, S S; Banville, D

    1994-07-01

    Upon fasting, an increase in proteolysis occurs in rat skeletal muscle and is associated with increased levels of ubiquitin-protein conjugates. As this suggests that formation of conjugates may be activated upon fasting, we studied the expression of the gene encoding the 14-kDa ubiquitin-conjugating enzyme (E2(14k)). A cDNA encoding rat E2(14k) was isolated and used to probe Northern blots of RNA from extensor digitorum longus muscles of fed, fasted, and refed rats. Two mRNA transcripts of 1.2 and 1.8 kb were observed. Isolation and sequencing of a genomic clone determined that these transcripts arise from differential sites of polyadenylation. The 1.2-kb transcript increased threefold upon fasting at 2 days and returned to normal with refeeding. Northern analysis of RNA from various tissues of fed and fasted rats showed that E2(14k) mRNA was expressed at high levels in testes, moderate levels in muscle, heart, and brain, but low levels in liver and kidney. Upon fasting, increases in mRNA levels were seen in muscle, heart, liver, and kidney. In vitro, in rat L6 myotubes, insulin lowered levels of E2(14k) mRNA. Because E2s catalyze the first irreversible reaction in the pathway and E2(14k) gene expression appears to change in parallel with the changes in levels of ubiquitinated proteins and rates of proteolysis, conjugation mediated by this E2 may be a rate-limiting step in the pathway. This is the first demonstration of direct hormonal regulation of a gene in the ubiquitin system and argues strongly for a role of the ubiquitin system in the metabolic response to fasting in skeletal muscle.

  19. Methods of Fast Exponentiation

    Directory of Open Access Journals (Sweden)

    Mohammed A. Maitah

    2010-01-01

    Full Text Available Problem statement: Modular exponentiation constitutes the basis of many well-known and widely used public key cryptosystems. Approach: A fast portable modular exponentiation algorithm considerably enhanced the speed and applicability of these systems, also an efficient implementation of this algorithm was the key to high performance of such system. Results: In this study, two main approaches for solving this problem were proposed. The proposed approaches involved calculations without usage of extra operational memory for saving constants and calculations with usage of preliminary calculated constants. Conclusion/Recommendations: The estimation of complexity of the speedup and effectiveness of proposed approaches for the data were presented.

  20. FAST NEUTRONIC REACTOR

    Science.gov (United States)

    Snell, A.H.

    1957-12-01

    This patent relates to a reactor and process for carrying out a controlled fast neutron chain reaction. A cubical reactive mass, weighing at least 920 metric tons, of uranium metal containing predominantly U/sup 238/ and having a U/sup 235/ content of at least 7.63% is assembled and the maximum neutron reproduction ratio is limited to not substantially over 1.01 by insertion and removal of a varying amount of boron, the reactive mass being substantially freed of moderator.

  1. Fasting and sport: an introduction.

    Science.gov (United States)

    Maughan, R J

    2010-06-01

    Most humans observe an overnight fast on a daily basis, and the human body copes well with short duration fasting. Periodic fasting is widely practised for cultural, religious or health reasons. Fasting may take many different forms. Prolonged restriction of food and fluid is harmful to health and performance, and it is often automatically assumed that intermittent fasting will lead to decrements in exercise performance. Athletes who choose to fast during training or competitions may therefore be at a disadvantage. The available evidence does not entirely support this view, but there is little or no information on the effects on elite athletes competing in challenging environments. Prolonged periods of training in the fasted state may not allow optimum adaptation of muscles and other tissues. Further research on a wide range of athletes with special nutrition needs is urgently required. In events where performance might be affected, other strategies to eliminate or minimise any effects must be sought.

  2. A disputed evidence on obesity: comparison of the effects of Rcan2 −/− and Rps6kb1 −/− mutations on growth and body weight in C57BL/6J mice* #

    Science.gov (United States)

    Zhao, Jing; Li, Shi-wei; Gong, Qian-qian; Ding, Ling-cui; Jin, Ye-cheng; Zhang, Jian; Gao, Jian-gang; Sun, Xiao-yang

    2016-01-01

    It is widely accepted that body weight and adipose mass are tightly regulated by homeostatic mechanisms, in which leptin plays a critical role through hypothalamic pathways, and obesity is a result of homeostatic disorder. However, in C57BL/6J mice, we found that Rcan2 increases food intake and plays an important role in the development of age-and diet-induced obesity through a leptin-independent mechanism. RCAN2 was initially identified as a thyroid hormone (T3)-responsive gene in human fibroblasts. Expression of RCAN2 is regulated by T3 through the PI3K-Akt/PKB-mTOR-Rps6kb1 signaling pathway. Intriguingly, both Rcan2 −/− and Rps6kb1 −/− mutations were reported to result in lean phenotypes in mice. In this study we compared the effects of these two mutations on growth and body weight in C57BL/6J mice. We observed reduced body weight and lower fat mass in both Rcan2 −/− and Rps6kb1 −/− mice compared to the wild-type mice, and we reported other differences unique to either the Rcan2 −/− or Rps6kb1 −/− mice. Firstly, loss of Rcan2 does not directly alter body length; however, Rcan2 −/− mice exhibit reduced food intake. In contrast, Rps6kb1 −/− mice exhibit abnormal embryonic development, which leads to smaller body size and reduced food intake in adulthood. Secondly, when fed a normal chow diet, Rcan2 −/− mice weigh significantly more than Rps6kb1 −/− mice, but both Rcan2 −/− and Rps6kb1−/− mice develop similar amounts of epididymal fat. On a high-fat diet, Rcan2 −/− mice gain body weight and fat mass at slower rates than Rps6kb1 −/− mice. Finally, using the double-knockout mice (Rcan2 −/− Rps6kb1 −/−), we demonstrate that concurrent loss of Rcan2 and Rps6kb1 has an additive effect on body weight reduction in C57BL/6J mice. Our data suggest that Rcan2 and Rps6kb1 mutations both affect growth and body weight of mice, though likely through different mechanisms. PMID:27604858

  3. The amiodarone derivative 2-methyl-3-(3,5-diiodo-4-carboxymethoxybenzyl)benzofuran (KB130015) opens large-conductance Ca2+-activated K+ channels and relaxes vascular smooth muscle.

    Science.gov (United States)

    Gessner, Guido; Heller, Regine; Hoshi, Toshinori; Heinemann, Stefan H

    2007-01-26

    2-methyl-3-(3,5-diiodo-4-carboxymethoxybenzyl)benzofuran (KB130015) has been developed to retain the antiarrhythmic properties of the parent molecule amiodarone but to eliminate its undesired side effects. In patch-clamp experiments, KB130015 activated large-conductance, Ca2+-activated BK(Ca) channels formed by hSlo1 (alpha) subunits in HEK 293 cells. Channels were reversibly activated by shifting the open-probability/voltage (P(o)/V) relationship by about -60 mV in 3 muM intracellular free Ca2+ ([Ca2+]in). No effect on the single-channel conductance was observed. KB130015-mediated activation of BK(Ca) channels was half-maximal at 20 microM with a Hill coefficient of 2.8. BK(Ca) activation by KB130015 did not require the presence of Ca2+ and still occurred with saturating (100 microM) [Ca2+]in. Effects of the prototypic BK(Ca) activator NS1619 (1,3-dihydro-1-[2-hydroxy-5-(trifluoromethyl)phenyl]-5-(trifluoromethyl)-2H-benzimidazol-2-one) and those of KB130015 were not additive suggesting that both activators may at least partially share a common mechanism of action. KB130015-mediated activation was observed also for BK(Ca) channels from insects and for human BK(Ca) channels with already profoundly left-shifted voltage-dependence. In contrast, human intermediate conductance Ca2+-activated channels were inhibited by KB130015. Using segments of porcine pulmonary arteries, KB130015 induced endothelium-independent vasorelaxation, half-maximal at 43 microM KB130015. Relaxation was inhibited by 1 mM tetraethylammonium, suggesting that KB130015 can activate vascular smooth muscle type BK(Ca) channels under physiological conditions. Interestingly, the shift in the P(o)/V relationship was considerably stronger (-90 mV in 3 microM [Ca2+]in) for BK(Ca) channels containing Slo-beta1 subunits. Thus, KB130015 belongs to a novel class of BK(Ca) channel openers that exert an effect depending on the subunit composition of the channel complex.

  4. Fast Fourier transform telescope

    Science.gov (United States)

    Tegmark, Max; Zaldarriaga, Matias

    2009-04-01

    We propose an all-digital telescope for 21 cm tomography, which combines key advantages of both single dishes and interferometers. The electric field is digitized by antennas on a rectangular grid, after which a series of fast Fourier transforms recovers simultaneous multifrequency images of up to half the sky. Thanks to Moore’s law, the bandwidth up to which this is feasible has now reached about 1 GHz, and will likely continue doubling every couple of years. The main advantages over a single dish telescope are cost and orders of magnitude larger field-of-view, translating into dramatically better sensitivity for large-area surveys. The key advantages over traditional interferometers are cost (the correlator computational cost for an N-element array scales as Nlog⁡2N rather than N2) and a compact synthesized beam. We argue that 21 cm tomography could be an ideal first application of a very large fast Fourier transform telescope, which would provide both massive sensitivity improvements per dollar and mitigate the off-beam point source foreground problem with its clean beam. Another potentially interesting application is cosmic microwave background polarization.

  5. Fast SCR Thyratron Driver

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen, M.N.; /SLAC

    2007-06-18

    As part of an improvement project on the linear accelerator at SLAC, it was necessary to replace the original thyratron trigger generator, which consisted of two chassis, two vacuum tubes, and a small thyratron. All solid-state, fast rise, and high voltage thyratron drivers, therefore, have been developed and built for the 244 klystron modulators. The rack mounted, single chassis driver employs a unique way to control and generate pulses through the use of an asymmetric SCR, a PFN, a fast pulse transformer, and a saturable reactor. The resulting output pulse is 2 kV peak into 50 {Omega} load with pulse duration of 1.5 {mu}s FWHM at 180 Hz. The pulse risetime is less than 40 ns with less than 1 ns jitter. Various techniques are used to protect the SCR from being damaged by high voltage and current transients due to thyratron breakdowns. The end-of-line clipper (EOLC) detection circuit is also integrated into this chassis to interrupt the modulator triggering in the event a high percentage of line reflections occurred.

  6. Disorders of red cell membrane

    National Research Council Canada - National Science Library

    An, Xiuli; Mohandas, Narla

    2008-01-01

    Summary Studies during the last three decades have enabled the development of detailed molecular insights into the structural basis of altered function in various inherited red cell membrane disorders...

  7. Red blood cells, spherocytosis (image)

    Science.gov (United States)

    Spherocytosis is a hereditary disorder of the red blood cells (RBCs), which may be associated with a mild anemia. Typically, the affected RBCs are small, spherically shaped, and lack the light centers seen ...

  8. A comparative transcriptional map of a region of 250 kb on the human and mouse X chromosome between the G6PD and the FLN1 genes.

    Science.gov (United States)

    Rivella, S; Tamanini, F; Bione, S; Mancini, M; Herman, G; Chatterjee, A; Maestrini, E; Toniolo, D

    1995-08-10

    The transcriptional organization of the region of the mouse X chromosome between the G6pd and the Fln1 genes was studied in detail, and it was compared with the syntenic region of the human chromosome. A cosmid contig of 250 kb was constructed by screening mouse cosmid libraries with probes for human genes and with whole cosmids. Overlapping cosmids were aligned by comparing EcoRI and rare-cutter restriction enzyme digestions. The gene order and the orientation of transcription were determined by hybridization with fragments from the 5' and 3' moieties of each cDNA. Our work demonstrates that all of the new genes identified in human are present in the mouse. The size of the region, 250 kb, is also very similar, as are gene order and gene organization: the transcriptional organization in "domains" described in human is found to be identical in the mouse. The major difference detected is the much lower content in rare-cutter restriction sites, which is related to the lower G+C and CpG content of mouse DNA. The very high conservation that we have described suggests that a potent selective pressure has contributed to such conservation of gene organization.

  9. A comparative transcriptional map of a region of 250 kb on the human and mouse X chromosome between the G6PD and the FLN1 genes

    Energy Technology Data Exchange (ETDEWEB)

    Rivella, S.; Tamanini, F.; Bione, S.; Mancini, M. [Istituto de Genetica Biochinica ed Evoluzionistica, Pavia (Italy)] [and others

    1995-08-10

    The transcriptional organization of the region of the mouse X chromosome between the G6pd and the Fln1 genes was studied in detail, and it was compared with the syntenic region of the human chromosome. A cosmid contig of 250 kb was constructed by screening mouse cosmid libraries with probes for human genes and with whole cosmids. Overlapping cosmids were aligned by comparing EcoRI and rare-cutter restriction enzyme digestions. The gene order and the orientation of transcription were determined by hybridization with fragments from the 5{prime} and 3{prime} moieties of each cDNA. Our work demonstrates that all of the new genes identified in human are present in the mouse. The size of the region, 250 kb, is also very similar, as are gene order and gene organizations: the transcriptional organization in {open_quotes}domains{close_quotes} described in human is found to be identical in the mouse. The major difference detected is the much lower content in rare-cutter restriction sites, which is related to the lower G+C and CpG content of mouse DNA. The very high conservation that we have described suggests that a potent selective pressure has contributed to such conservation of gene organization. 17 refs., 4 figs.

  10. The human interactome knowledge base (hint-kb): An integrative human protein interaction database enriched with predicted protein–protein interaction scores using a novel hybrid technique

    KAUST Repository

    Theofilatos, Konstantinos A.

    2013-07-12

    Proteins are the functional components of many cellular processes and the identification of their physical protein–protein interactions (PPIs) is an area of mature academic research. Various databases have been developed containing information about experimentally and computationally detected human PPIs as well as their corresponding annotation data. However, these databases contain many false positive interactions, are partial and only a few of them incorporate data from various sources. To overcome these limitations, we have developed HINT-KB (http://biotools.ceid.upatras.gr/hint-kb/), a knowledge base that integrates data from various sources, provides a user-friendly interface for their retrieval, cal-culatesasetoffeaturesofinterest and computesaconfidence score for every candidate protein interaction. This confidence score is essential for filtering the false positive interactions which are present in existing databases, predicting new protein interactions and measuring the frequency of each true protein interaction. For this reason, a novel machine learning hybrid methodology, called (Evolutionary Kalman Mathematical Modelling—EvoKalMaModel), was used to achieve an accurate and interpretable scoring methodology. The experimental results indicated that the proposed scoring scheme outperforms existing computational methods for the prediction of PPIs.

  11. Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing.

    Science.gov (United States)

    Vembar, Shruthi Sridhar; Seetin, Matthew; Lambert, Christine; Nattestad, Maria; Schatz, Michael C; Baybayan, Primo; Scherf, Artur; Smith, Melissa Laird

    2016-08-01

    The application of next-generation sequencing to estimate genetic diversity of Plasmodium falciparum, the most lethal malaria parasite, has proved challenging due to the skewed AT-richness [∼80.6% (A + T)] of its genome and the lack of technology to assemble highly polymorphic subtelomeric regions that contain clonally variant, multigene virulence families (Ex: var and rifin). To address this, we performed amplification-free, single molecule, real-time sequencing of P. falciparum genomic DNA and generated reads of average length 12 kb, with 50% of the reads between 15.5 and 50 kb in length. Next, using the Hierarchical Genome Assembly Process, we assembled the P. falciparum genome de novo and successfully compiled all 14 nuclear chromosomes telomere-to-telomere. We also accurately resolved centromeres [∼90-99% (A + T)] and subtelomeric regions and identified large insertions and duplications that add extra var and rifin genes to the genome, along with smaller structural variants such as homopolymer tract expansions. Overall, we show that amplification-free, long-read sequencing combined with de novo assembly overcomes major challenges inherent to studying the P. falciparum genome. Indeed, this technology may not only identify the polymorphic and repetitive subtelomeric sequences of parasite populations from endemic areas but may also evaluate structural variation linked to virulence, drug resistance and disease transmission.

  12. Analysis of plasmid diversity in 96 Rhodococcus equi strains isolated in Normandy (France) and sequencing of the 87-kb type I virulence plasmid.

    Science.gov (United States)

    Duquesne, Fabien; Hébert, Laurent; Sévin, Corinne; Breuil, Marie-France; Tapprest, Jackie; Laugier, Claire; Petry, Sandrine

    2010-10-01

    To characterize the potential epidemiological relationship between the origin of Rhodococcus equi strains and the type of their virulence plasmids, we performed a comparative analysis of virulence plasmid types encountered in 96 R. equi strains isolated from (1) autopsied horses, (2) organic samples (horse faeces, manure and straw) and (3) environmental samples. Our results revealed no clear epidemiological link between virulence plasmid type and the origin of R. equi strains isolated from horse-related environments. To understand this result, we determined the nucleotide sequence of the second most frequently isolated virulence plasmid type: a 87-kb type I (pVAPA116) plasmid and compared it with the previously sequenced (and most commonly encountered) 85-kb type I (pVAPA1037) plasmid. Our results show that the divergence between these two plasmids is mainly due to the presence of three allelic exchange loci, resulting in the deletion of two genes and the insertion of three genes in pVAPA116 compared with pVAPA1037. In conclusion, it appears that the divergence between the two sequenced rhodococcal virulence plasmids is not associated with the vap pathogenicity island and may result from an evolutionary process driven by a mobility-related invertase/resolvase invA-like gene. © 2010 ANSES. Journal compilation © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.

  13. Isolation of a 97-kb minimal essential MHC B locus from a new reverse-4D BAC library of the golden pheasant.

    Directory of Open Access Journals (Sweden)

    Qing Ye

    Full Text Available The bacterial artificial chromosome (BAC system is widely used in isolation of large genomic fragments of interest. Construction of a routine BAC library requires several months for picking clones and arraying BACs into superpools in order to employ 4D-PCR to screen positive BACs, which might be time-consuming and laborious. The major histocompatibility complex (MHC is a cluster of genes involved in the vertebrate immune system, and the classical avian MHC-B locus is a minimal essential one, occupying a 100-kb genomic region. In this study, we constructed a more effective reverse-4D BAC library for the golden pheasant, which first creates sub-libraries and then only picks clones of positive sub-libraries, and identified several MHC clones within thirty days. The full sequencing of a 97-kb reverse-4D BAC demonstrated that the golden pheasant MHC-B locus contained 20 genes and showed good synteny with that of the chicken. The notable differences between these two species were the numbers of class II B loci and NK genes and the inversions of the TAPBP gene and the TAP1-TAP2 region. Furthermore, the inverse TAP2-TAP1 was unique in the golden pheasant in comparison with that of chicken, turkey, and quail. The newly defined genomic structure of the golden pheasant MHC will give an insight into the evolutionary history of the avian MHC.

  14. Organization, structure and evolution of 41kb of genomic DNA spanning the D-J-C region of the sheep TRB locus.

    Science.gov (United States)

    Antonacci, R; Di Tommaso, S; Lanave, C; Cribiu, E P; Ciccarese, S; Massari, S

    2008-01-01

    A genomic region of 41,045 bp encompassing the 3'-end of the sheep T cell receptor beta chain was sequenced. Extensive molecular analysis has revealed that this region retains a unique structural feature for the presence of a third D-J-C cluster, never detected in any other mammalian species examined so far. A total of 3 TRBD, 18 TRBJ and 3 substantially identical TRBC genes were identified in about 28kb. At 13kb, downstream from the last TRBC gene, in an inverted transcriptional orientation, lies a TRBV gene. Sequence comparison and phylogenetic analyses have demonstrated that the extra D-J-C cluster originated from an unequal crossing over between the two ancestral TRBC genes. Interspersed repeats spanning 22.2% of the sequence, contribute to the wider size of the sheep TRB locus with respect to the other mammalian counterparts, without modifying the general genomic architecture. The nucleotide and predicted amino acid sequences from peripheral T cells cDNA clones indicated that the genes from cluster 3 are fully implicated in the beta chain recombination machinery. Closer inspections of the transcripts have also shown that inter-cluster rearrangements and splice variants, involving the additional cluster, increase the functional diversity of the sheep beta chain repertoire.

  15. Catalog of 162 single nucleotide polymorphisms (SNPs) in a 4.7-kb region of the HLA-DP loci in southern Chinese ethnic groups

    Institute of Scientific and Technical Information of China (English)

    XiaoyiLiu; ZehuanLiu; BinLin; YuanyuanLiu; ZhuoxunChen; WeicongHe; DongZhong; AnlongXu

    2005-01-01

    HLA class-Ⅱ proteins are cell-surface molecules that present antigens to T cells, and their expressional regulation is crucial to the immune reaction.Sequence variation at the regulatory region can directly affect the gene expression level. We cloned and sequenced a 4.7-kb region containing the regulatory region, exonl, and partial intronl of both HLA-DPA1 and DPB1 genes in 25 variable sequences from southern Chinese ethnic groups and got a high-density map of162 single nucleotide polymorphisms (SNPs): seven in 5′-flanking regions, four in 5′-untranslated regions, and four in the coding regions. By comparing these data with SNPs in dbSNP database in the NCBI, 145 SNPs(89.5%) were novel. In addition, eight genetic variations of insertion-deletion polymorphisms (INDELs) were discovered within the 4.7-kb region. These high-resolution maps can be used as resources of markers for association studies of complex diseases, assessment of individuals' predisposition to diseases, and tailoring oftherapies, as well as research markers for population genetics and evolution.

  16. Red supergiants and stellar evolution

    CERN Document Server

    Ekström, Sylvia; Meynet, Georges; Groh, Jose; Granada, Anahí

    2013-01-01

    We review the significant role played by red supergiants (RSGs) in stellar populations, and some challenges and questions they raise for theoretical stellar evolution. We present how metallicity and rotation modify the way stars go to the red part of the Hertzsprung- Russell diagram or come back from it, and how RSGs might keep a trace of their main-sequence evolution. We compare theoretical popu- lation ratios with observed ones.

  17. Red Tide off Texas Coast

    Science.gov (United States)

    2002-01-01

    Red tides (algae) bloomed late this summer along a 300-mile stretch of Texas' Gulf Coast, killing millions of fish and shellfish as well as making some people sick. State officials are calling this the worst red tide bloom in 14 years. The algae produces a poison that paralyzes fish and prevents them from breathing. There is concern that the deadly algae could impact or even wipe out this year's oyster harvest in Texas, which usually peaks during the Thanksgiving and Christmas holidays. The red tides were first observed off the Texas coast in mid-August and have been growing steadily in size ever since. Red tides tend to bloom and subside rapidly, depending upon changes in wind speed and direction, water temperature, salinity, and rainfall patterns (as the algae doesn't do as well in fresher water). This true-color image of the Texas Gulf Coast was acquired on September 29, 2000, by the Moderate-resolution Imaging Spectroradiometer (MODIS) flying aboard NASA's Terra spacecraft. The red tide can be seen as the dark reddish discoloration in the ocean running southwest to northeast along the coast. In this scene, the bloom appears to be concentrated north and east of Corpus Christi, just off Matagorda Island. The image was made at 500-meter resolution using a combination of MODIS' visible bands 1 (red), 4 (green), and 3 (blue). The city of Houston can be seen clearly as the large, greyish cluster of pixels to the north and west of Galveston Bay, which is about mid-way up the coastline in this image. Also visible in this image are plumes of smoke, perhaps wildfires, both to the north and northeast of Houston. For more information about red tides, refer to the Texas Red Tide Web site. Image courtesy Andrey Savtchenko, MODIS Data Support Team, and the MODIS Ocean Team, NASA's Goddard Space Flight Center

  18. Fast Food Jobs. National Study of Fast Food Employment.

    Science.gov (United States)

    Charner, Ivan; Fraser, Bryna Shore

    A study examined employment in the fast-food industry. The national survey collected data from employees at 279 fast-food restaurants from seven companies. Female employees outnumbered males by two to one. The ages of those fast-food employees in the survey sample ranged from 14 to 71, with fully 70 percent being in the 16- to 20-year-old age…

  19. Islamic fasting and multiple sclerosis

    Science.gov (United States)

    2014-01-01

    Background Month-long daytime Ramadan fasting pose s major challenges to multiple sclerosis (MS) patients in Muslim countries. Physicians should have practical knowledge on the implications of fasting on MS. We present a summary of database searches (Cochrane Database of Systematic Reviews, PubMed) and a mini-symposium on Ramadan fasting and MS. In this symposium, we aimed to review the effect of fasting on MS and suggest practical guidelines on management. Discussion In general, fasting is possible for most stable patients. Appropriate amendment of drug regimens, careful monitoring of symptoms, as well as providing patients with available evidence on fasting and MS are important parts of management. Evidence from experimental studies suggests that calorie restriction before disease induction reduces inflammation and subsequent demyelination and attenuates disease severity. Fasting does not appear to have unfavorable effects on disease course in patients with mild disability (Expanded Disability Status Scale (EDSS) score ≤3). Most experts believed that during fasting (especially in summer), some MS symptoms (fatigue, fatigue perception, dizziness, spasticity, cognitive problems, weakness, vision, balance, gait) might worsen but return to normal levels during feasting. There was a general consensus that fasting is not safe for patients: on high doses of anti-convulsants, anti-spastics, and corticosteroids; with coagulopathy or active disease; during attacks; with EDSS score ≥7. Summary These data suggest that MS patients should have tailored care. Fasting in MS patients is a challenge that is directly associated with the spiritual belief of the patient. PMID:24655543

  20. Ramadan fasting, pregnancy and lactation

    Directory of Open Access Journals (Sweden)

    Mohsen Khoshniat Nikoo

    2014-04-01

    Full Text Available Background: Fasting and malnutrition during pregnancy is associated with deleterious consequences such as hypoglycemia, ketonemia, impaired fetal IQ, low birth weight and even abortion. Comparison of pregnancy length and year duration shows that about 75% of pregnancies coincided with Ramadan. Also, fasting during Ramadan is not equivalent to hunger and malnutrition, however, knowledge of the effects of Ramadan fasting on pregnancy outcome is important. In this review, the results of all studies related to the possible effects of Ramadan fasting in pregnancy and lactation have been collected. Material and Methods: Keywords such as "Ramadan", "Ramadan Fasting", "Islamic Fasting", "Fasting in Ramadan "and Fasting with words Pregnancy, Birth Weight, Lactation, Preterm, Milk Composition, Breast Milk were searched in PubMed Database, SID (Scientific Information Database, and some regional journals and 40 related articles (descriptive cross - sectional, cohort, clinical trial and review articles from 1968 to 2010 were studied. Results: Based on available information, if the maternal nutrition during Ramadan is good, the normal process of pregnancy will be maintained and Ramadan fasting would not have deleterious effects on fetal physical and mental growth. Conclusion: Considering nutritional tips, nursing mothers could also fast during Ramadan.

  1. Beyond Fast Mapping.

    Science.gov (United States)

    Carey, Susan

    2010-07-08

    Since the seminal 1957 studies of word learning by Roger Brown, most experimental studies of lexical acquisition have concerned fast mapping: the process through which a new lexical entry is established, and through which representations of the linguistic context of a newly heard word interact with representations of its nonlinguistic context to fix an initial partial meaning. Here I focus on the subsequent extended process through which the adult meaning is approximated. Two factors lead to an extended learning process; the size of the hypothesis space and the need, sometimes, for the creation of new semantic primitives. Sometimes lexical learning requires conceptual change. I sketch a learning mechanism through which this can be achieved. A case study of learning the meanings of verbal numerals illustrates the argument.

  2. Materials analysis fast ions

    CERN Document Server

    Denker, A; Rauschenberg, J; Röhrich, J; Strub, E

    2006-01-01

    Materials analysis with ion beams exploits the interaction of ions with the electrons and nuclei in the sample. Among the vast variety of possible analytical techniques available with ion beams we will restrain to ion beam analysis with ion beams in the energy range from one to several MeV per mass unit. It is possible to use either the back-scattered projectiles (RBS – Rutherford Back Scattering) or the recoiled atoms itself (ERDA – Elastic Recoil Detection Analysis) from the elastic scattering processes. These techniques allow the simultaneous and absolute determination of stoichiometry and depth profiles of the detected elements. The interaction of the ions with the electrons in the sample produces holes in the inner electronic shells of the sample atoms, which recombine and emit X-rays characteristic for the element in question. Particle Induced X-ray Emission (PIXE) has shown to be a fast technique for the analysis of elements with an atomic number above 11.

  3. Habitability of planets around red dwarf stars.

    Science.gov (United States)

    Heath, M J; Doyle, L R; Joshi, M M; Haberle, R M

    1999-08-01

    Recent models indicate that relatively moderate climates could exist on Earth-sized planets in synchronous rotation around red dwarf stars. Investigation of the global water cycle, availability of photosynthetically active radiation in red dwarf sunlight, and the biological implications of stellar flares, which can be frequent for red dwarfs, suggests that higher plant habitability of red dwarf planets may be possible.

  4. Avian pectoral muscle size rapidly tracks body mass changes during flight, fasting and fuelling

    NARCIS (Netherlands)

    Lindstrom, A; Kvist, A; Piersma, T; Dekinga, A; Dietz, MW; Lindström, Åke

    2000-01-01

    We used ultrasonic imaging to monitor short-term changes in the pectoral muscle size of captive red knots Calidris canutus. Pectoral muscle thickness changed rapidly and consistently in parallel with body mass changes caused by flight, fasting;and fuelling. Four knots hew repeatedly for 10h periods

  5. Fast reactor programme in India

    Indian Academy of Sciences (India)

    P Chellapandi; P R Vasudeva Rao; Prabhat Kumar

    2015-09-01

    Role of fast breeder reactor (FBR) in the Indian context has been discussed with appropriate justification. The FBR programme since 1985 till 2030 is highlighted focussing on the current status and future direction of fast breeder test reactor (FBTR), prototype fast breeder reactor (PFBR) and FBR-1 and 2. Design and technological challenges of PFBR and design and safety targets with means to achieve the same are the major highlights of this paper.

  6. Synthesis and characterization of natural red dye from Caesalpinia sappan linn

    Energy Technology Data Exchange (ETDEWEB)

    Mulyanto, Subur, E-mail: subur.mulyanto@poltekba.ac.id [Graduate Program of Mechanical Engineering, SebelasMaret University, Jl. Ir. Sutami 36 A, Surakarta (Indonesia); Department of Mechanical Engineering, State Polytechnic of Balikpapan, Jl. Soekarno-Hatta Km.8 Balikpapan (Indonesia); Suyitno,, E-mail: suyitno@uns.ac.id; Rachmanto, Rendy Adhi, E-mail: rendy.ar@gmail.com; Hidayat, Lullus Lambang Govinda, E-mail: lulus-l@yahoo.com; Hadi, Syamsul, E-mail: syamsulhadi@ft.uns.ac.id [Department of Mechanical Engineering, SebelasMaret University, Jl. Ir. Sutami 36 A, Surakarta (Indonesia); Wibowo, Atmanto Heru, E-mail: aheruwibowo@yahoo.com [Department of Chemistry, SebelasMaret University, Jl. Ir. Sutami 36 A, Surakarta (Indonesia)

    2016-03-29

    The study reports the synthesis and characterization of natural red dye. The dyes were extracted from woods of Caesalpiniasappanlinn at varied temperatures of 70, 80, 90, and 100°C for three hours. The dry wood chips and water at a ratio of 6:1 were immersed in the reactor of 150 liters. The absorbance spectra of the natural red dyes were measured by ultra-violet-visible spectroscopy. Meanwhile, Fourier transform infrared spectroscopy was used to investigate the functional groups of the natural red dyes. In addition, the basic production cost was calculated and the fastness property towards cotton fabrics was investigated according to the Indonesia national standard of 105-C06:2010, 105-B01:2010, and 0288-2008. The results showed that the functional groups found the extracted red dyes indicated the complex bond of brazilein with peak absorbance at a wavelength of 538-540 nm. The extraction temperature also changed the functional group of brazilein. From the color, the absorbance peak, the functional groups, and the main production cost, the best parameter to synthesize the natural red dyes from Caesalpiniasappanlinn was at a temperature of 80°C for two hours. Moreover, the natural red dyes has the fastness to wash resistance, light resistance, and scrub resistance by 4-5, 4, and 3-4, respectively. However, further studies for synthesis the natural red dyes by using a continuous reactor are required to identify the naturally complex compounds in brazilein for improving the fastness properties and for reducing the cost.

  7. Synthesis and characterization of natural red dye from Caesalpinia sappan linn

    Science.gov (United States)

    Mulyanto, Subur; Suyitno, Rachmanto, Rendy Adhi; Hidayat, Lullus Lambang Govinda; Wibowo, Atmanto Heru; Hadi, Syamsul

    2016-03-01

    The study reports the synthesis and characterization of natural red dye. The dyes were extracted from woods of Caesalpiniasappanlinn at varied temperatures of 70, 80, 90, and 100°C for three hours. The dry wood chips and water at a ratio of 6:1 were immersed in the reactor of 150 liters. The absorbance spectra of the natural red dyes were measured by ultra-violet-visible spectroscopy. Meanwhile, Fourier transform infrared spectroscopy was used to investigate the functional groups of the natural red dyes. In addition, the basic production cost was calculated and the fastness property towards cotton fabrics was investigated according to the Indonesia national standard of 105-C06:2010, 105-B01:2010, and 0288-2008. The results showed that the functional groups found the extracted red dyes indicated the complex bond of brazilein with peak absorbance at a wavelength of 538-540 nm. The extraction temperature also changed the functional group of brazilein. From the color, the absorbance peak, the functional groups, and the main production cost, the best parameter to synthesize the natural red dyes from Caesalpiniasappanlinn was at a temperature of 80°C for two hours. Moreover, the natural red dyes has the fastness to wash resistance, light resistance, and scrub resistance by 4-5, 4, and 3-4, respectively. However, further studies for synthesis the natural red dyes by using a continuous reactor are required to identify the naturally complex compounds in brazilein for improving the fastness properties and for reducing the cost.

  8. Neuropsychological phenotype of a patient with a de novo 970 kb interstitial deletion in the distal 16p11.2 region

    Directory of Open Access Journals (Sweden)

    Egger JI

    2014-03-01

    Full Text Available Jos I M Egger,1–3 Willem M A Verhoeven,1,4 Wim Verbeeck,5 Nicole de Leeuw61Vincent van Gogh Institute for Psychiatry, Centre of Excellence for Neuropsychiatry, Venray, the Netherlands; 2Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, the Netherlands; 3Behavioural Science Institute, Radboud University Nijmegen, Nijmegen, the Netherlands; 4Erasmus University Medical Centre, Department of Psychiatry, Rotterdam, the Netherlands; 5Vincent van Gogh Institute for Psychiatry, Centre for Autism and ADHD, Venray, the Netherlands; 6Department of Human Genetics, Radboud University Medical Centre, Nijmegen, the NetherlandsAbstract: The 16p11.2 microdeletion syndrome is characterized by a wide range of phenotypic expressions and is frequently associated with developmental delay, symptoms from the autism spectrum, epilepsy, congenital anomalies, and obesity. These phenotypes are often related to a proximal 16p11.2 deletion of approximately 600 kb (BP4–BP5 that includes the SH2B1 gene that is reported to be causative for morbid obesity. This more centromeric deletion is most strongly related to autism spectrum susceptibility and is functionally different from the more distal 16p12.2p11.2 region, which includes the so-called atypical 16p11.2 BP2–BP3 deletion (approximately 220 kb presenting with developmental delay, behavioral problems and mild facial dysmorphisms. Here, an adult male with a long history of maladaptive behaviors is described who was referred for diagnostic assessment of his amotivational features. Extensive neuropsychological examination demonstrated rigid thinking, anxious beliefs, and ideas of reference in the presence of normal intelligence. Microarray analysis demonstrated a de novo 970 kb 16p11.2 BP1–BP4 microdeletion that can be regarded as explanatory for his behavioral profile. It is concluded that microdeletion syndromes are not exclusively related to intellectual disabilities and

  9. Red cell metabolism studies on Skylab

    Science.gov (United States)

    Mengel, C. E.

    1977-01-01

    Blood samples from Spacelab crewmembers were studied for possible environment effects on red cell components. Analysis involved peroxidation of red cell lipids, enzymes of red cell metabolism, and levels of 2,3-diphosphoglyceric acid and adenosine triphosphate. Results show that there is no evidence of lipid peroxidation, that biochemical effect known to be associated with irreversible red cell damage. Changes observed in glycolytic intermediates and enzymes cannot be directly implicated as indicating evidence of red cell damage.

  10. Red cell metabolism studies on Skylab

    Science.gov (United States)

    Mengel, C. E.

    1977-01-01

    Blood samples from Spacelab crewmembers were studied for possible environment effects on red cell components. Analysis involved peroxidation of red cell lipids, enzymes of red cell metabolism, and levels of 2,3-diphosphoglyceric acid and adenosine triphosphate. Results show that there is no evidence of lipid peroxidation, that biochemical effect known to be associated with irreversible red cell damage. Changes observed in glycolytic intermediates and enzymes cannot be directly implicated as indicating evidence of red cell damage.

  11. Nitric Oxide Scavenging by Red Cell Microparticles

    OpenAIRE

    Liu, Chen; Zhao, Weixin; George J Christ; Gladwin, Mark T.; Kim-Shapiro, Daniel B.

    2013-01-01

    Red cell microparticles form during the storage of red blood cells and in diseases associated with red cell breakdown and asplenia, including hemolytic anemias such as sickle cell disease. These small phospholipid vesicles that are derived from red blood cells have been implicated in the pathogenesis of transfusion of aged stored blood and hemolytic diseases, via activation of the hemostatic system and effects on nitric oxide (NO) bioavailability. Red cell microparticles react with the import...

  12. 76 FR 22033 - Safety Zone; Red River Safety Zone, Red River, MN

    Science.gov (United States)

    2011-04-20

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AAOO Safety Zone; Red River Safety Zone, Red River, MN AGENCY... Safety Unit Duluth, MN is establishing a temporary safety zone on the Red River, MN. This safety zone is...-0263 to read as follows: Sec. 165.T09-0263 Safety zone; Red River Safety Zone, Red River, MN. (a...

  13. Pensar la ciudad en red

    Directory of Open Access Journals (Sweden)

    Fábio Duarte

    2006-01-01

    Full Text Available El paradigma de la sociedad contemporánea es la red, como afirma Manuel Castells (1999 cuándo propone este concepto para que se piense el mundo frente a las tecnologías de información y comunicación. ¿Más como pensar la ciudad en red? Cuándo vemos una imagen aérea de una ciudad, con sus rutas que articulan puntos y regiones, tenemos una imagen perfecta de una red. Pero la evidencia absoluta entre la forma geométrica de una red no puede nos dejar iludirnos: esto no presupone el entendimiento de la sociedad urbana como red. El matemático Nikos Salingaros (2003 argumentó que “las fuerzas que hacen que la ciudad funcione son generadas por la diversidad y necesidad de cambio de información entre diferentes tipos de nodos”, y Gabriel Dupuy (1985 escribió que “si buscamos localizar partes de un sistema, debemos hacerlo en espacios abstractos, espacios topológicos, espacios de n dimensiones, inhabituales en el planeamiento y que no corresponden a la percepción inmediata de la ‘redes clásicas’. Así, las redes son, más que nada, una manera de pensar. Una manera de leer el mundo, una manera de actuar en el mundo.

  14. Fast word reading in pure alexia: "fast, yet serial".

    Science.gov (United States)

    Bormann, Tobias; Wolfer, Sascha; Hachmann, Wibke; Neubauer, Claudia; Konieczny, Lars

    2015-01-01

    Pure alexia is a severe impairment of word reading in which individuals process letters serially with a pronounced length effect. Yet, there is considerable variation in the performance of alexic readers with generally very slow, but also occasionally fast responses, an observation addressed rarely in previous reports. It has been suggested that "fast" responses in pure alexia reflect residual parallel letter processing or that they may even be subserved by an independent reading system. Four experiments assessed fast and slow reading in a participant (DN) with pure alexia. Two behavioral experiments investigated frequency, neighborhood, and length effects in forced fast reading. Two further experiments measured eye movements when DN was forced to read quickly, or could respond faster because words were easier to process. Taken together, there was little support for the proposal that "qualitatively different" mechanisms or reading strategies underlie both types of responses in DN. Instead, fast responses are argued to be generated by the same serial-reading strategy.

  15. Biological oceanography of the red oceanic system

    Science.gov (United States)

    Theil, Hjalmar; Weikert, Horst

    species' adaptations to their environment this temperature influence will apply to all the living components of the ecosystem. Hence for Red Sea organisms maintenance costs are comparatively high, which greatly reduces their production potential. 7. Thus the oceanic ecosystem of the Red Sea can be summarised as follows: Primary production is low throughout most of the year and throughout most of the entire basin, and consequently so is secondary production (zooplankton). In subsurface waters, high maintenance costs reduces energy for investments in growth and reproduction. In addition degradation of organic matter is so fast in the upper layers that the standing stock in the intermediate layers is reduced and particle transport to greater depth in the central graben greatly restricted. The low abundance and biomass of the deep-sea plankton and benthos in the Red Sea is readily explained by the low availability of food and the high metabolic demands created by the unique high in situ temperature.

  16. Gas chromatography/mass spectrometry for the determination of nitrosamines in red wine.

    Science.gov (United States)

    Lona-Ramirez, Fernando J; Gonzalez-Alatorre, Guillermo; Rico-Ramírez, Vicente; Perez-Perez, Ma Cristina I; Castrejón-González, Edgar O

    2016-04-01

    N-nitrosamines (NAms) are highly active carcinogens that have been detected in food and beverages. Currently certain studies report their presence in red wine, while others fail to detect their presence. In this study the head space solid phase micro-extraction technique coupled to gas chromatography-mass spectrometry (HS-SPME-GC-MS) was applied to quantify four NAms in different types of red wine. The technique was found to be a simple, precise, fast and environmentally friendly alternative for the quantification of volatile NAms. A factorial analysis was carried out to evaluate the influence of the parameters on the HS-SPME technique. This is the first study that such analysis has been reported and where NAms in red wine have been quantified using HS-SPME-GC-MS. The method was validated by calculating the linearity, limit of detection and quantification. Two of the four NAms analyzed were found to be present in red wine samples.

  17. Congenital nasal pyriform aperture stenosis and ocular albinism co-occurring in a sibship with a maternally-inherited 97 kb Xp22.2 microdeletion.

    Science.gov (United States)

    Somsen, David; Davis-Keppen, Laura; Crotwell, Patricia; Flanagan, Jason; Munson, Patrick; Stein, Quinn

    2014-05-01

    Congenital Nasal Pyriform Aperture Stenosis (CNPAS) is a rare congenital malformation caused by overgrowth of the maxillary bone. We report on two patients, brothers born 3 and 1½ years apart, both presented at birth with radiographically diagnosed CNPAS. Both siblings also were born with ocular albinism, which is known to have X-linked inheritance. Subsequent genetic testing demonstrated a 97 kb deletion in the p arm of the X chromosome in both siblings and their mother. This deletion encompasses a gene known to cause ocular albinism (GPR143), as well as partial deletion of two other genes, TBL1X and SHROOM2. This is the first reported case of CNPAS in siblings, both males, sharing a maternally inherited Xp22.2 deletion.

  18. Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression.

    Science.gov (United States)

    Rivella, S; Palermo, B; Pelizon, C; Sala, C; Arrigo, G; Toniolo, D

    1999-11-15

    In higher eukaryotes the mechanism controlling initiation of DNA replication remains largely unknown. New technologies are needed to shed light on how DNA replication initiates along the genome in specific regions. To identify the human DNA sequence requirements for initiation of replication, we developed a new method that allows selection of replication origins starting from large genomic regions of human DNA. We repeatedly isolated 15 new putative replication origins (PROs) from a human DNA region of 500 kb in which 17 genes have previously been characterized. Fine-mapping of these PROs showed that DNA replication can initiate at many specific points along actively transcribed DNA in the cell lines used for our selection. In conclusion, in this paper we describe a new method to identify PROs that suggests that the availability of initiation sites is dependent on the transcriptional state of the DNA.

  19. The human zinc-finger protein-7 gene is located 90 kb 3' of MYC and is not expressed in Burkitt lymphoma cell lines.

    Science.gov (United States)

    Feduchi, E; Gallego, M I; Lazo, P A

    1994-09-15

    The zinc-finger gene-7 (ZNF7) was located 90 kb 3' of MYC on human chromosome 8 band q24 by pulsed-field gel electrophoresis (PFGE). This position lies between the MLV14 and BVR1 loci, 2 variant translocation breakpoints in Burkitt lymphomas. The structure of the ZNF7 gene was not altered by translocations in Burkitt-lymphoma cell lines as shown by its germline-restriction map configuration. The chromosomal region surrounding the ZNF7 gene was extensively methylated. The ZNF7 gene was not expressed in 19 BL cell lines. Expression was detected only in the BL41 and BL47 cell lines and in the SW756 cervical-carcinoma cell line. The RNA in each was of a different size. We postulate that the lack of ZNF7 expression in Burkitt lymphomas might contribute to the tumor phenotype.

  20. Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8 kb DNA fragment of yeast chromosome VII.

    Science.gov (United States)

    Tizon, B; Rodríguez-Torres, M; Rodríguez-Belmonte, E; Cadahia, J L; Cerdan, E

    1996-09-01

    We report the sequence analysis of a 6.8 kb DNA fragment from Saccharomyces cerevisiae chromosome VII. This sequence contains five open reading frames (ORFs) greater than 100 amino acids. There is also an incomplete ORF flanking one of the extremes, G2868, which is the 3' end of the SCS3 gene (Hosaka et al., 1994). The translated sequence of ORF G2882 shows similarity to the human methylenetetrahydrofolate reductase (Goyette et al., 1994). ORF G2889 shows no significant homologies with the sequences compiled in databases. ORF G2893 corresponds to the gene SUP44, coding for the yeast ribosomal protein S4 (All-Robin et al., 1990). G2873 and G2896 are internal ORFs.

  1. Effects of Hull Scratching, Soaking, and Boiling on Antinutrients in Japanese Red Sword Bean (Canavalia gladiata).

    Science.gov (United States)

    Une, Satsuki; Nonaka, Koji; Akiyama, Junich

    2016-10-01

    The effects of hull processing, soaking, and boiling on the content or activity of antinutrients in the red sword bean (RSB; Canavalia gladiata) were investigated. RSB seeds were compared with kidney bean (KB; Phaseolus vulgaris) seeds that are starch based and often used as processed products in Japan. RSB seeds had higher weight, thicker hull, and higher protein content, but lower moisture content compared with KB seeds. Because of the strong and thick hull, the relative water absorption of untreated RSB seeds was very low after soaking. Seeds were soaked after dehulling, scratching, and roasting. The results showed that hull scratching was the optimal method for increasing water absorption during soaking compared with dehulling and roasting. After soaking, the water used for soaking was discarded, since it had a high content of polyphenols and bitter taste, and RSB seeds were boiled in fresh water for 20, 40, and 60 min. The results showed that polyphenol and tannin contents, antioxidant activity, and hemagglutinating activity, as well as maltase, sucrase, and trypsin inhibitor activities in scratched RSB seeds decreased significantly after boiling compared with those in raw seeds, whereas amylase inhibitor activity showed no significant change. Overall, it was concluded that the combination of hull scratching, soaking, and boiling in fresh water can reduce thermal-stable or sensitive antinutrients in RSB and thus, significantly improve its nutritional value. © 2016 Institute of Food Technologists®.

  2. Using the Neuroadaptagen KB200z™ to Ameliorate Terrifying, Lucid Nightmares in RDS Patients: the Role of Enhanced, Brain-Reward, Functional Connectivity and Dopaminergic Homeostasis.

    Science.gov (United States)

    McLaughlin, Thomas; Blum, Kenneth; Oscar-Berman, Marlene; Febo, Marcelo; Demetrovics, Zsolt; Agan, Gozde; Fratantonio, James; Gold, Mark S

    Lucid Dreams are a form of dream life, during which the dreamer may be aware that he/she is dreaming, can stop/re-start the dreams, depending on the pleasantness or unpleasant nature of the dream, and experiences the dream as if he/she were fully awake. Depending on their content, they may be pleasant, un-pleasant or terrifying, at least in the context of patients, who also exhibit characteristics of Reward Deficiency Syndrome (RDS) and Posttraumatic Stress Disorder (PTSD). We present eight clinical cases, with known substance abuse, childhood abuse and diagnosed PTSD/RDS. The administration of a putative dopamine agonist, KB200Z™, was associated with the elimination of unpleasant and/or terrifying, lucid dreams in 87.5% of the cases presented, whereas one very heavy cocaine abuser showed a minimal response. These results required the continuous use of this nutraceutical. The lucid dreams themselves were distinguishable from typical, PTSD nightmares insofar as their content did not appear to reflect a symbolic rendition of an originally-experienced, historical trauma. Each of the cases was diagnosed with a form of RDS, i.e., ADHD, ADD, and/or Tourette's syndrome. They all also suffered from some form of Post-Traumatic-Stress-Disorder (PTSD) and other psychiatric diagnoses as well. The reduction or elimination of terrifying Lucid Dreams seemed to be dependent on KB220Z, whereby voluntary stopping of the agent results in reinstatement of the terrifying non-pleasant nature of the dreams. Following more required research on a much larger population we anticipate confirmation of these seemingly interesting observations. If these results in a small number of patients are indeed confirmed we may have found a frontline solution to a very perplexing and complicated symptom known as lucid dreams.

  3. Using the Neuroadaptagen KB200z™ to Ameliorate Terrifying, Lucid Nightmares in RDS Patients: the Role of Enhanced, Brain-Reward, Functional Connectivity and Dopaminergic Homeostasis

    Directory of Open Access Journals (Sweden)

    Thomas McLaughlin

    2015-03-01

    Full Text Available Background: Lucid Dreams are a form of dream life, during which the dreamer may be aware that he/she is dreaming, can stop/re-start the dreams, depending on the pleasantness or unpleasant nature of the dream, and experiences the dream as if he/she were fully awake. Depending on their content, they may be pleasant, un-pleasant or terrifying, at least in the context of patients, who also exhibit characteristics of Reward Deficiency Syndrome (RDS and Posttraumatic Stress Disorder (PTSD. Case Series: We present eight clinical cases, with known substance abuse, childhood abuse and diagnosed PTSD/RDS. The administration of a putative dopamine agonist, KB200Z™, was associated with the elimination of unpleasant and/or terrifying, lucid dreams in 87.5% of the cases presented, whereas one very heavy cocaine abuser showed a minimal response. These results required the continuous use of this nutraceutical. The lucid dreams themselves were distinguishable from typical, PTSD nightmares insofar as their content did not appear to reflect a symbolic rendition of an originally-experienced, historical trauma. Each of the cases was diagnosed with a form of RDS, i.e., ADHD, ADD, and/or Tourette’s syndrome. They all also suffered from some form of Post- Traumatic-Stress-Disorder (PTSD and other psychiatric diagnoses as well. Conclusion: The reduction or elimination of terrifying Lucid Dreams seemed to be dependent on KB220Z, whereby voluntary stopping of the agent results in reinstatement of the terrifying non-pleasant nature of the dreams. Following more required research on a much larger population we anticipate confirmation of these seemingly interesting observations. If these results in a small number of patients are indeed confirmed we may have found a frontline solution to a very perplexing and complicated symptom known as lucid dreams.

  4. Identification of a novel first exon in the human dystrophin gene and of a new promoter located more than 500 kb upstream of the nearest known promoter

    Energy Technology Data Exchange (ETDEWEB)

    Yanagawa, H.; Nishio, H.; Takeshima, Y. [Kobe Univ. School of Medicine (Japan)] [and others

    1994-09-01

    The dystrophin gene, which is muted in patients with Duchenne and Becker muscular dystrophies, is the largest known human gene. Five alternative promoters have been characterized until now. Here we show that a novel dystrophin isoform with a different first exon can be produced through transcription initiation at a previously-unidentified alternative promoter. The case study presented is that of patient with Duchenne muscular dystrophy who had a deletion extending from 5{prime} end of the dystrophin gene to exon 2, including all promoters previously mapped in the 5{prime} part of the gene. Transcripts from lymphoblastoid cells were found to contain sequences corresponding to exon 3, indicating the presence of new promoter upstream of this exon. The nucleotide sequence of amplified cDNA corresponding to the 5{prime} end of the new transcript indicated that the 5{prime} end of exon 3 was extended by 9 codons, only the last (most 3{prime}) of which codes for methionine. The genomic nucleotide sequence upstream from the new exon, as determined using inverse polymerase chain reaction, revealed the presence of sequences similar to a TATA box, an octamer motif and an MEF-2 element. The identified promoter/exon did not map to intron 2, as might have been expected, but to a position more than 500 kb upstream of the most 5{prime} of the previously-identified promoters, thereby adding 500 kb to the dystrophin gene. The sequence of part of the new promoter region is very similar to that of certain medium reiteration frequency repetitive sequences. These findings may help us understand the molecular evolution of the dystrophin gene.

  5. Chronic low-dose radiation protects cells from high-dose radiation via increase of AKT expression by NF-{sub k}B

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hyung Sun; Seong, Ki Moon; Kim, Ji Young; Kim, Cha Soon; Yang, Kwang Hee; Nam, Seon Young [Radiation Effect Research Team, Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., LTD., Gyeongju (Korea, Republic of)

    2013-04-15

    Exposure to low-dose and low-dose rate of ionizing radiation is an important issue in radiation protection. Low-dose ionizing radiation has been observed to elicit distinctly different responses compared to high-dose radiation, in various biological systems including the reproductive, immune, and hematopoietic systems (Liu et al. 2006). Some data were reported that low-dose radiation could initiate beneficial effects by stimulating cell growth, DNA repair, activation of transcription factors and gene expression (Calabrese et al., 2004). Cells exposed to low-dose radiation can develop adaptive resistance to subsequent high-dose radiation induced DNA damage, gene mutation, and cell death. We previously reported that low-dose of ionizing radiation induced cell survival through the activation of AKT (protein kinase B, PKB) pathway (Park et al., 2009). AKT has been shown to be potently activated in response to a wide variety of growth factors and ionizing radiation. Cell survival against ionizing radiation seems to be associated with the activation of AKT pathway via phosphorylation of its downstream nuclear target molecules. In the present study, we examined the effects of chronic low-dose irradiation in human lung fibroblast cells. The aim was to explore the possibility of a low-dose radiation-induced adaptive cellular response against subsequent challenging high-dose irradiation. In the present study, we examined the regulatory mechanism responsible for cellular response induced by chronic low-dose of ionizing radiation in normal human cells. We found that the level of AKT protein was closely associated with cell survival. In addition, NF-{sub k}B activation by chronic low-dose radiation regulates AKT activation via gene expression and acinus expression. In conclusion, our data demonstrate that chronic low-dose radiation could inhibit the cell death induced by cytotoxic high-dose radiation through the modulation of the level of AKT and acinus proteins via NF-{sub k}B

  6. Genome-wide linkage and copy number variation analysis reveals 710 kb duplication on chromosome 1p31.3 responsible for autosomal dominant omphalocele

    Science.gov (United States)

    Radhakrishna, Uppala; Nath, Swapan K; McElreavey, Ken; Ratnamala, Uppala; Sun, Celi; Maiti, Amit K; Gagnebin, Maryline; Béna, Frédérique; Newkirk, Heather L; Sharp, Andrew J; Everman, David B; Murray, Jeffrey C; Schwartz, Charles E; Antonarakis, Stylianos E; Butler, Merlin G

    2017-01-01

    Background Omphalocele is a congenital birth defect characterised by the presence of internal organs located outside of the ventral abdominal wall. The purpose of this study was to identify the underlying genetic mechanisms of a large autosomal dominant Caucasian family with omphalocele. Methods and findings A genetic linkage study was conducted in a large family with an autosomal dominant transmission of an omphalocele using a genome-wide single nucleotide polymorphism (SNP) array. The analysis revealed significant evidence of linkage (non-parametric NPL = 6.93, p=0.0001; parametric logarithm of odds (LOD) = 2.70 under a fully penetrant dominant model) at chromosome band 1p31.3. Haplotype analysis narrowed the locus to a 2.74 Mb region between markers rs2886770 (63014807 bp) and rs1343981 (65757349 bp). Molecular characterisation of this interval using array comparative genomic hybridisation followed by quantitative microsphere hybridisation analysis revealed a 710 kb duplication located at 63.5–64.2 Mb. All affected individuals who had an omphalocele and shared the haplotype were positive for this duplicated region, while the duplication was absent from all normal individuals of this family. Multipoint linkage analysis using the duplication as a marker yielded a maximum LOD score of 3.2 at 1p31.3 under a dominant model. The 710 kb duplication at 1p31.3 band contains seven known genes including FOXD3, ALG6, ITGB3BP, KIAA1799, DLEU2L, PGM1, and the proximal portion of ROR1. Importantly, this duplication is absent from the database of genomic variants. Conclusions The present study suggests that development of an omphalocele in this family is controlled by overexpression of one or more genes in the duplicated region. To the authors’ knowledge, this is the first reported association of an inherited omphalocele condition with a chromosomal rearrangement. PMID:22499347

  7. Responder fast steering mirror

    Science.gov (United States)

    Bullard, Andrew; Shawki, Islam

    2013-10-01

    Raytheon Space and Airborne Systems (SAS) has designed, built and tested a 3.3-inch diameter fast steering mirror (FSM) for space application. This 2-axis FSM operates over a large angle (over 10 degree range), has a very high servo bandwidth (over 3.3 Khz closed loop bandwidth), has nanoradian-class noise, and is designed to support microradian class line of sight accuracy. The FSM maintains excellent performance over large temperature ranges (which includes wave front error) and has very high reliability with the help of fully redundant angle sensors and actuator circuits. The FSM is capable of achieving all its design requirements while also being reaction-compensated. The reaction compensation is achieved passively and does not need a separate control loop. The FSM has undergone various environmental testing which include exported forces and torques and thermal vacuum testing that support the FSM design claims. This paper presents the mechanical design and test results of the mechanism which satisfies the rigorous vacuum and space application requirements.

  8. Fast dual tomography

    Science.gov (United States)

    Carrion, Philip M.

    1990-09-01

    This paper can be considered as a continuation of the work by Carrion and Carneiro (1989), where a generalized approach to linearized inversion of geophysical data was developed. Their method allows one to incorporate virtually any constraints in the inversion and reformulate the problem in the dual space of Langrangian multipliers (see also Carrion, 1989a). The constrained tomography makes traveltime inversion robust: it automatically rejects “bad data” which correspond to solutions beyond the chosen constraints and allows one to start inversion with an arbitrary chosen initial model.In this paper, I will derive basic formulas for constrained tomographic imaging that can be used in such areas of geophysics as global mapping of the earth interior, exploration geophysics, etc. The method is fast: an example that will be shown in the paper took only 6 min. of VAX CPU time. Had the conventional least-squares matrix inversion been used it would have taken more than 10 hours of the CPU time to solve the same problem.

  9. Fast dual tomography

    Energy Technology Data Exchange (ETDEWEB)

    Carrion, P.M. (PPPG/UFBA - Campus Universitario da Federacao, Salvador-Bahia (Brazil))

    1990-09-01

    This paper can be considered as a continuation of the work by Carrion and Carneiro (1989), where a generalized approach to linearized inversion of geophysical data was developed. Their method allows one to incorporate virtually any constraints in the inversion and reformulate the problem in the dual space of Langrangian multipliers (see also Carrion, 1989a). The constrained tomography makes traveltime inversion robust: it automatically rejects bad data which correspond to solutions beyond the chosen constraints and allows one to start inversion with an arbitrary chosen initial model. In this paper, the author derives basic formulas for constrained tomographic imaging that can be used in such areas of geophysics as global mapping of the earth interior, exploration geophysics, etc. The method is fast: an example that will be shown in the paper took only 6 min. of VAX CPU time. Had the conventional least-squares matrix inversion been used it would have taken more than 10 hours of the CPU time to solve the same problem.

  10. The ATLAS Fast Tracker

    CERN Document Server

    Volpi, Guido; The ATLAS collaboration

    2015-01-01

    The use of tracking information at the trigger level in the LHC Run II period is crucial for the trigger an data acquisition (TDAQ) system. The tracking precision is in fact important to identify specific decay products of the Higgs boson or new phenomena, a well as to distinguish the contributions coming from many contemporary collisions that occur at every bunch crossing. However, the track reconstruction is among the most demanding tasks performed by the TDAQ computing farm; in fact, full reconstruction at full Level-1 trigger accept rate (100 KHz) is not possible. In order to overcome this limitation, the ATLAS experiment is planning the installation of a specific processor: the Fast Tracker (FTK), which is aimed at achieving this goal. The FTK is a pipeline of high performance electronic, based on custom and commercial devices, which is expected to reconstruct, with high resolution, the trajectories of charged tracks with a transverse momentum above 1 GeV, using the ATLAS inner tracker information. Patte...

  11. Fast Flooding over Manhattan

    CERN Document Server

    Clementi, Andrea; Silvestri, Riccardo

    2010-01-01

    We consider a Mobile Ad-hoc NETwork (MANET) formed by n agents that move at speed V according to the Manhattan Random-Way Point model over a square region of side length L. The resulting stationary (agent) spatial probability distribution is far to be uniform: the average density over the "central zone" is asymptotically higher than that over the "suburb". Agents exchange data iff they are at distance at most R within each other. We study the flooding time of this MANET: the number of time steps required to broadcast a message from one source agent to all agents of the network in the stationary phase. We prove the first asymptotical upper bound on the flooding time. This bound holds with high probability, it is a decreasing function of R and V, and it is tight for a wide and relevant range of the network parameters (i.e. L, R and V). A consequence of our result is that flooding over the sparse and highly-disconnected suburb can be as fast as flooding over the dense and connected central zone. Rather surprisin...

  12. Fast Aerial Video Stitching

    Directory of Open Access Journals (Sweden)

    Jing Li

    2014-10-01

    Full Text Available The highly efficient and robust stitching of aerial video captured by unmanned aerial vehicles (UAVs is a challenging problem in the field of robot vision. Existing commercial image stitching systems have seen success with offline stitching tasks, but they cannot guarantee high-speed performance when dealing with online aerial video sequences.In this paper, we present a novel system which has an unique ability to stitch high-frame rate aerial video at a speed of 150 frames per second (FPS. In addition, rather than using a high-speed vision platform such as FPGA or CUDA, our system is running on a normal personal computer. To achieve this, after the careful comparison of the existing invariant features, we choose the FAST corner and binary descriptor for efficient feature extraction and representation, and present a spatial and temporal coherent filter to fuse the UAV motion information into the feature matching. The proposed filter can remove the majority of feature correspondence outliers and significantly increase the speed of robust feature matching by up to 20 times. To achieve a balance between robustness and efficiency, a dynamic key frame-based stitching framework is used to reduce the accumulation errors.Extensive experiments on challenging UAV datasets demonstrate that our approach can break through the speed limitation and generate an accurate stitching image for aerial video stitching tasks.

  13. Fast feedback in classroom practice

    NARCIS (Netherlands)

    Emmett, K.M.; Klaassen, K.; Eijkelhof, H.

    2009-01-01

    In this article we describe one application of the fast feedback method (see Berg 2003 Aust. Sci. Teach. J. 28–34) in secondary mechanics education. Two teachers tried out a particular sequence twice, in consecutive years, once with and once without the use of fast feedback. We found the method to b

  14. Fasting and nonfasting lipid levels

    DEFF Research Database (Denmark)

    Langsted, Anne; Freiberg, Jacob J; Nordestgaard, Børge G

    2008-01-01

    Lipid profiles are usually measured after fasting. We tested the hypotheses that these levels change only minimally in response to normal food intake and that nonfasting levels predict cardiovascular events.......Lipid profiles are usually measured after fasting. We tested the hypotheses that these levels change only minimally in response to normal food intake and that nonfasting levels predict cardiovascular events....

  15. Fasting and nonfasting lipid levels

    DEFF Research Database (Denmark)

    Langsted, Anne; Freiberg, Jacob J; Nordestgaard, Børge G

    2008-01-01

    Lipid profiles are usually measured after fasting. We tested the hypotheses that these levels change only minimally in response to normal food intake and that nonfasting levels predict cardiovascular events.......Lipid profiles are usually measured after fasting. We tested the hypotheses that these levels change only minimally in response to normal food intake and that nonfasting levels predict cardiovascular events....

  16. Glycemic management during Jain fasts

    OpenAIRE

    Sandeep Julka; Alok Sachan; Sarita Bajaj; Rakesh Sahay; Rajeev Chawla; Navneet Agrawal; Banshi Saboo; Unnikrishnan, A. G.; Baruah, Manash P.; Girish Parmar; Sanjay Kalra

    2017-01-01

    This review describes the various fasts observed by adherents of the Jain religion. It attempts to classify them according to their suitability for people with diabetes and suggests appropriate regime and dose modification for those observing these fasts. The review is an endeavor to encourage rational and evidence-based management in this field of diabetology.

  17. Oil Analysis by Fast DSC

    NARCIS (Netherlands)

    Wetten, I.A.; Herwaarden, A.W.; Splinter, R.; Ruth, van S.M.

    2014-01-01

    Thermal analysis of Olive and Sunflower Oil is done by Fast DSC to evaluate its potential to replace DSC for adulteration detection. DSC measurements take hours, Fast DSC minutes. Peak temperatures of the crystallisation peak in cooling for different Olive and Sunflower Oils are both comparable to D

  18. Oil Analysis by Fast DSC

    NARCIS (Netherlands)

    Wetten, I.A.; Herwaarden, A.W.; Splinter, R.; Ruth, van S.M.

    2014-01-01

    Thermal analysis of Olive and Sunflower Oil is done by Fast DSC to evaluate its potential to replace DSC for adulteration detection. DSC measurements take hours, Fast DSC minutes. Peak temperatures of the crystallisation peak in cooling for different Olive and Sunflower Oils are both comparable to

  19. Fast Feedback in Classroom Practice

    Science.gov (United States)

    Emmett, Katrina; Klaassen, Kees; Eijkelhof, Harrie

    2009-01-01

    In this article we describe one application of the fast feedback method (see Berg 2003 "Aust. Sci. Teach. J." 28-34) in secondary mechanics education. Two teachers tried out a particular sequence twice, in consecutive years, once with and once without the use of fast feedback. We found the method to be successful, and the data that we obtained…

  20. Seguridad en una red universitaria

    OpenAIRE

    Bernal España, Juan Antonio

    2014-01-01

    Las redes de comunicaciones son muy importantes para las empresas. Se solicita una red de altas prestaciones que pueda llevar muchos sistemas sobre ella (cámaras de seguridad, video, voz, datos, SCADA, wifi). Ahora también necesitamos que la red sea segura. Cuando hablamos de seguridad no solo nos referimos a evitar ataques o virus, también hablamos de cómo puede afectarnos el incendio de un centro de proceso de datos. Basándonos en la ISO 27001:2013 daremos las principales pau...

  1. Estrategias de trabajo en red

    OpenAIRE

    Cabral, María Cristina

    2006-01-01

    El presente informe fue elaborado en el marco del proyecto de investigación “Sentidos en las Estrategias de comunicación / Educación de las ONGs y el Estado dirigidas a los adolescentes de Viedma”. Aborda los debates en torno a las estrategias de intervención en lo social en trabajo en red, a partir de la experiencia de la Red Adolescencia Viedma. Además, de analizar las formas de participación, control e incidencia de la Sociedad Civil y el rol del Estado en las políticas sociales. Facult...

  2. Red Plague Control Plan (RPCP)

    Science.gov (United States)

    Cooke, Robert W.

    2010-01-01

    SCOPE: Prescribes the minimum requirements for the control of cuprous / cupric oxide corrosion (a.k.a. Red Plague) of silver-coated copper wire, cable, and harness assemblies. PURPOSE: Targeted for applications where exposure to assembly processes, environmental conditions, and contamination may promote the development of cuprous / cupric oxide corrosion (a.k.a. Red Plague) in silver-coated copper wire, cable, and harness assemblies. Does not exclude any alternate or contractor-proprietary documents or processes that meet or exceed the baseline of requirements established by this document. Use of alternate or contractor-proprietary documents or processes shall require review and prior approval of the procuring NASA activity.

  3. Eye Redness Image Processing Techniques

    Science.gov (United States)

    Adnan, M. R. H. Mohd; Zain, Azlan Mohd; Haron, Habibollah; Alwee, Razana; Zulfaezal Che Azemin, Mohd; Osman Ibrahim, Ashraf

    2017-09-01

    The use of photographs for the assessment of ocular conditions has been suggested to further standardize clinical procedures. The selection of the photographs to be used as scale reference images was subjective. Numerous methods have been proposed to assign eye redness scores by computational methods. Image analysis techniques have been investigated over the last 20 years in an attempt to forgo subjective grading scales. Image segmentation is one of the most important and challenging problems in image processing. This paper briefly outlines the comprehensive of image processing and the implementation of image segmentation in eye redness.

  4. Nonlinear analysis of RED - a comparative study

    Energy Technology Data Exchange (ETDEWEB)

    Jiang Kai; Wang Xiaofan E-mail: xfwang@sjtu.edu.cn; Xi Yugeng

    2004-09-01

    Random Early Detection (RED) is an active queue management (AQM) mechanism for routers on the Internet. In this paper, performance of RED and Adaptive RED are compared from the viewpoint of nonlinear dynamics. In particular, we reveal the relationship between the performance of the network and its nonlinear dynamical behavior. We measure the maximal Lyapunov exponent and Hurst parameter of the average queue length of RED and Adaptive RED, as well as the throughput and packet loss rate of the aggregate traffic on the bottleneck link. Our simulation scenarios include FTP flows and Web flows, one-way and two-way traffic. In most situations, Adaptive RED has smaller maximal Lyapunov exponents, lower Hurst parameters, higher throughput and lower packet loss rate than that of RED. This confirms that Adaptive RED has better performance than RED.

  5. Dynamo-generated magnetic fields in fast rotating single giants

    CERN Document Server

    Konstantinova-Antova, Renada; Schröder, Klaus-Peter; Petit, Pascal

    2009-01-01

    Red giants offer a good opportunity to study the interplay of magnetic fields and stellar evolution. Using the spectro-polarimeter NARVAL of the Telescope Bernard Lyot (TBL), Pic du Midi, France and the LSD technique, we began a survey of magnetic fields in single G-K-M giants. Early results include 6 MF-detections with fast rotating giants, and for the first time a magnetic field was detected directly in an evolved M-giant: EK Boo. Our results could be explained in the terms of $\\alpha$--$\\omega$ dynamo operating in these giants.

  6. White Wedding and Red Wedding

    Institute of Scientific and Technical Information of China (English)

    李梦遥

    2010-01-01

    @@ The most obvious difference between western wedding and Chinese wedding tradition lies in the main color of the ceremony.Western wedding is known as the white wedding in some sense,for its main color is white from bride's gown(礼服)to the decoration of the wedding hall.While,traditional Chinese wedding ceremony is full of the color of red.

  7. Site curves for red alder.

    Science.gov (United States)

    Daniel M. Bishop; Floyd A. Johnson

    1958-01-01

    The increasing commercial importance of red alder (Alnus rubra) in the Pacific Northwest has created a demand for research on this species. Noting the lack of information on growth of alder, the Puget Sound Research Center Advisory Committee established a subcommittee in January 1956 to undertake construction of alder yield tables. Through the...

  8. Advances in Red VCSEL Technology

    Directory of Open Access Journals (Sweden)

    Klein Johnson

    2012-01-01

    Full Text Available Red VCSELs offer the benefits of improved performance and lower power consumption for medical and industrial sensing, faster printing and scanning, and lower cost, higher speed interconnects based upon plastic optical fiber (POF. However, materials challenges make it more difficult to achieve the desired performance than at the well-developed wavelength of 850 nm. This paper will describe the state of the art of red VCSEL performance and the results of development efforts to achieve improved output power and a broader temperature range of operation. It will also provide examples of the applications of red VCSELs and the benefits they offer. In addition, the packaging flexibility offered by VCSELs, and some examples of non-Hermetic package demonstrations will be discussed. Some of the red VCSEL performance demonstrations include output power of 14 mW CW at room temperature, a record maximum temperature of 115∘C for CW operation at an emission wavelength of 689 nm, time to 1% failure at room temperature of approximately 200,000 hours, lifetime in a 50∘C, 85% humidity environment in excess of 3500 hours, digital data rate of 3 Gbps, and peak pulsed array power of greater than 100 mW.

  9. Fast food: unfriendly and unhealthy.

    Science.gov (United States)

    Stender, S; Dyerberg, J; Astrup, A

    2007-06-01

    Although nutrition experts might be able to navigate the menus of fast-food restaurant chains, and based on the nutritional information, compose apparently 'healthy' meals, there are still many reasons why frequent fast-food consumption at most chains is unhealthy and contributes to weight gain, obesity, type 2 diabetes and coronary artery disease. Fast food generally has a high-energy density, which, together with large portion sizes, induces over consumption of calories. In addition, we have found it to be a myth that the typical fast-food meal is the same worldwide. Chemical analyses of 74 samples of fast-food menus consisting of French fries and fried chicken (nuggets/hot wings) bought in McDonalds and KFC outlets in 35 countries in 2005-2006 showed that the total fat content of the same menu varies from 41 to 65 g at McDonalds and from 42 to 74 g at KFC. In addition, fast food from major chains in most countries still contains unacceptably high levels of industrially produced trans-fatty acids (IP-TFA). IP-TFA have powerful biological effects and may contribute to increased weight gain, abdominal obesity, type 2 diabetes and coronary artery disease. The food quality and portion size need to be improved before it is safe to eat frequently at most fast-food chains.

  10. Still red light for red light cameras? An update.

    Science.gov (United States)

    Høye, Alena

    2013-06-01

    The present study has replicated the results from a previous meta-analysis by Erke (2009) [Erke, A., 2009. Red light for red-light cameras? A meta-analysis of the effects of red-light cameras on crashes. Accident Analysis & Prevention 41 (5), 897-905.] based on a larger sample of RLC-studies, and provides answers to the criticisms that were raised by Lund et al. (2009) [Lund, A.K., Kyrychenko, S.Y., Retting, R.A., 2009. Caution: a comment on Alena Erke's red light for red-light cameras? A meta-analysis of the effects of red-light cameras on crashes. Accident Analysis and Prevention 41, 895-896.] against the previous meta-analysis. The addition of recent studies to the meta-analysis and a more thorough investigation of potential moderator variables lead to a slight improvement of the estimated effects of RLC in the previous meta-analysis. The present study found a non-significant increase of all crashes by 6% and a non-significant decrease of all injury crashes by 13%. Right-angle collisions were found to decrease by 13% and rear-end collisions were found to increase by 39%. For right-angle injury collisions a decrease by 33% was found and for rear-end injury collisions a smaller increase was found (+19%). The effects of RLC are likely to be more favorable when RLC-warning signs are set up at main entrances to areas with RLC enforcement than when each RLC-intersection is signposted. The effects of RLC may become more favorable over time, this could however not be investigated empirically. Several results indicate that spillover effects may occur for right-angle collisions, but most likely not for rear-end and other crashes. If spillover effects do not occur for rear-end crashes, which increase at RLC intersection, this would be a positive result for RLC. However, the results seem to be affected to some degree by publication bias and the effects may therefore be somewhat less favorable than indicated by the results from meta-analysis.

  11. FastID: Extremely Fast Forensic DNA Comparisons

    Science.gov (United States)

    2017-05-19

    FastID: Extremely Fast Forensic DNA Comparisons Darrell O. Ricke, PhD Bioengineering Systems & Technologies Massachusetts Institute of...Technology Lincoln Laboratory Lexington, MA USA Darrell.Ricke@ll.mit.edu Abstract—Rapid analysis of DNA forensic samples can have a critical impact on...time sensitive investigations. Analysis of forensic DNA samples by massively parallel sequencing is creating the next gold standard for DNA

  12. fast,rapid,quick和speedy

    Institute of Scientific and Technical Information of China (English)

    张德兴

    2003-01-01

    fast,rapid,quick和speedy这组词都有“快的”意思,但将它们放在一起略作议论,仍有必要,并不乏词趣。 fast和rapid虽然有时可以互换使用,彼此仍有差异,fast在作形容词的时候,强调物体进行相对运动。如a fast horse,a fast train.除形容词外,fast可做副词。如:

  13. Molecular imaging with fast beams

    Energy Technology Data Exchange (ETDEWEB)

    Heber, O. [Weizmann Inst. of Science, Rehovoth (Israel). Dept. of Particle Physics; Zajfman, D. [Weizmann Inst. of Science, Rehovoth (Israel). Dept. of Particle Physics; Kella, D. [Weizmann Inst. of Science, Rehovoth (Israel). Dept. of Particle Physics; Vager, Z. [Weizmann Inst. of Science, Rehovoth (Israel). Dept. of Particle Physics; Watson, R.L. [Cyclotron Institute, Texas A and M University, College Station, Texas 77843 (United States); Horvat, V. [Cyclotron Institute, Texas A and M University, College Station, Texas 77843 (United States)

    1995-05-01

    Three dimensional imaging of the molecular dissociation process in fast collisions is presented with two different setups. One setup is for a fast molecular beam from an accelerator colliding with a gas target. The second setup is for a molecular target system and the collision process is with highly ionized fast beam. The advantages of each system are discussed. The three dimensional imaging of the molecular fragments is done with special detectors that combine the CCD image with time of flight data. An example of the molecular beam measurement is given for an 11 MeV B{sub 2} beam. (orig.).

  14. A putative autonomous 20.5 kb-CACTA transposon insertion in an F3'H allele identifies a new CACTA transposon subfamily in Glycine max

    Directory of Open Access Journals (Sweden)

    Vodkin Lila

    2008-12-01

    Full Text Available Abstract Background The molecular organization of very few genetically defined CACTA transposon systems have been characterized thoroughly as those of Spm/En in maize, Tam1 of Antirrhinum majus Candystripe1 (Cs1 from Sorghum bicolor and CAC1 from Arabidopsis thaliana, for example. To date, only defective deletion derivatives of CACTA elements have been described for soybean, an economically important plant species whose genome sequence will be completed in 2008. Results We identified a 20.5 kb insertion in a soybean flavonoid 3'-hydroxylase (F3'H gene representing the t* allele (stable gray trichome color whose origin traces to a single mutable chimeric plant displaying both tawny and gray trichomes. This 20.5 kb insertion has the molecular structure of a putative autonomous transposon of the CACTA family, designated Tgmt*. It encodes a large gene that was expressed in two sister isolines (T* and tm of the stable gray line (t* from which Tgmt* was isolated. RT-PCR derived cDNAs uncovered the structure of a large precursor mRNA as well as alternatively spliced transcripts reminiscent of the TNPA-mRNA generated by the En-1 element of maize but without sequence similarity to the maize TNPA. The larger mRNA encodes a transposase with a tnp2 and TNP1-transposase family domains. Because the two soybean lines expressing Tgmt* were derived from the same mutable chimeric plant that created the stable gray trichome t* allele line from which the element was isolated, Tgmt* has the potential to be an autonomous element that was rapidly inactivated in the stable gray trichome t* line. Comparison of Tgmt* to previously described Tgm elements demonstrated that two subtypes of CACTA transposon families exist in soybean based on divergence of their characteristic subterminal repeated motifs and their transposases. In addition, we report the sequence and annotation of a BAC clone containing the F3'H gene (T locus which was interrupted by the novel Tgmt* element

  15. Non-tuberculous Mycobacteriosis with T-cell Lymphoma in a Red Panda (Ailurus fulgens).

    Science.gov (United States)

    Fuke, N; Hirai, T; Makimura, N; Goto, Y; Habibi, W A; Ito, S; Trang, N T; Koshino, K; Takeda, M; Yamaguchi, R

    2016-01-01

    A 9-year-old male red panda (Ailurus fulgens) became emaciated and died. Necropsy examination revealed systemic lymphadenomegaly. The liver, lungs and left kidney contained multifocal yellow nodules. Microscopical examination revealed granulomatous inflammation in the liver, lungs, kidney, spleen and lymph nodes, with numerous acid-fast bacilli. Sequencing of genetic material isolated from the tissues classified the pathogen as Mycobacterium gastri. Lymphoma was found in the liver, lungs, kidney and lymph nodes. The neoplastic cells were strongly labelled for expression of CD3, Ki67 and proliferating cell nuclear antigen by immunohistochemistry. This is the first report of M. gastri infection with T-cell lymphoma in a red panda.

  16. Red blood cells, sickle cell (image)

    Science.gov (United States)

    Sickle cell anemia is an inherited blood disease in which the red blood cells produce abnormal pigment (hemoglobin). ... abnormal hemoglobin causes deformity of the red blood cells into crescent or sickle-shapes, as seen in this photomicrograph.

  17. Sirt1 overexpression protects murine osteoblasts against TNF-α-induced injury in vitro by suppressing the NF-KB signaling pathway

    Institute of Scientific and Technical Information of China (English)

    Wei HUANG; Wei-lin SHANG; Hua-dong WANG; Wen-wen WU; Shu-xun HOU

    2012-01-01

    Sirtuin 1 (Sirt1) is the class III histone/protein deacetylase that interferes with the NF-KB signaling pathway,thereby has antiinflammatory function.This study was undertaken to investigate whether Sirt1 could protect osteoblasts against TNF-α-induced injury in vitro.Methods:Murine osteoblastic cell line,MC3T3-E1,was used.Overexpress of Sirt1 protein in MC3T3-E1 cells was made by transfection the cells with Sirt1-overexpressing adenovirus.The levels of mRNAs and proteins were determined with qRT-PCR and Western blotting,respectively.The activity of NF-κB was examined using NF-κB luciferase assay.The NO concentration was measured using the Griess method.Results:Treatment of MC3T3-E1 cells with TNF-α (2.5-10 ng/mL) suppressed Sirt1 protein expression in a concentration-dependent manner.TNF-α (5 ng/mL) resulted in an increase in apoptosis and a reduction in ALP activity in the cells.Overexpression of Sirt1in the cells significantly attenuated TNF-α-induced injury through suppressing apoptosis,increasing ALP activity,and increasing the expression of Runx2 and osteocalcin mRNAs.Furthermore,overexpression of Sirt1 in the cells significantly suppressed TNF-α-induced NF-κB activation,followed by reducing the expression of iNOS and NO formation.Sirt1 activator resveratrol (10 μmol/L) mimicked the protection of the ceils by Sirt1 overexpression against TNF-α-induced injury,which was reversed by the Sirt1 inhibitor EX-527 (5μmol/L).Conclusion:Overexpression of Sirt1 protects MC3T3-E1 osteoblasts aganst TNF-α-induced cell injury in vitro,at least in part,via suppressing NF-KB signaling.Sirt1 may be a novel therapeutic target for treating rheumatoid arthritis-related bone loss.

  18. In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis.

    Directory of Open Access Journals (Sweden)

    Hong Guo

    Full Text Available The murine Cebpa gene contains a +37 kb, evolutionarily conserved 440 bp enhancer that directs high-level expression to myeloid progenitors in transgenic mice. The enhancer is bound and activated by Runx1, Scl, GATA2, C/EBPα, c-Myb, Pu.1, and additional Ets factors in myeloid cells. CRISPR/Cas9-mediated replacement of the wild-type enhancer with a variant mutant in its seven Ets sites leads to 20-fold reduction of Cebpa mRNA in the 32Dcl3 myeloid cell line. To determine the effect of deleting the enhancer in vivo, we now characterize C57BL/6 mice in which loxP sites flank a 688 bp DNA segment containing the enhancer. CMV-Cre mediated germline deletion resulted in diminution of the expected number of viable Enh(f/f;CMV-Cre offspring, with 28-fold reduction in marrow Cebpa mRNA but normal levels in liver, lung, adipose, intestine, muscle, and kidney. Cre-transduction of lineage-negative marrow cells in vitro reduced Cebpa mRNA 12-fold, with impairment of granulocytic maturation, morphologic blast accumulation, and IL-3 dependent myeloid colony replating for >12 generations. Exposure of Enh(f/f;Mx1-Cre mice to pIpC led to 14-fold reduction of Cebpa mRNA in GMP or CMP, 30-fold reduction in LSK, and <2-fold reduction in the LSK/SLAM subset. FACS analysis of marrow from these mice revealed 10-fold reduced neutrophils, 3-fold decreased GMP, and 3-fold increased LSK cells. Progenitor cell cycle progression was mildly impaired. Granulocyte and B lymphoid colony forming units were reduced while monocytic and erythroid colonies were increased, with reduced Pu.1 and Gfi1 and increased Egr1 and Klf4 in GMP. Finally, competitive transplantation indicated preservation of functional long-term hematopoietic stem cells upon enhancer deletion and confirmed marrow-intrinsic impairment of granulopoiesis and B cell generation with LSK and monocyte lineage expansion. These findings demonstrate a critical role for the +37 kb Cebpa enhancer for hematopoietic

  19. A Socio-Economic and Environmental Information Needs Knowledge Base (SEE-IN KB) in Support of SDG Implementation and Monitoring

    Science.gov (United States)

    Plag, H. P.; Jules-Plag, S.

    2016-12-01

    The UN Agenda 2030 has seventeen Sustainable Development Goals (SDGs) to be reach by 2030, which are detailed in 170 Targets. A monitioring framework of 240 SDG Indicators provides the metrics to measure progress towards these targets. The SDG Indicators are report cards for the progress towards the targets and a measure to assess potential impacts of policies and other means in support of SDG implementation. The Socio-Economic and Environmental Information Needs Knowledge Base (SEE-IN KB) collects information on objects such as user types, applications, observational requirements, a number of needs, societal goals and targets, indicators and indices, models, services, and datasets, as well as the interconnections between these objects, including links to Essential Variables (EVs). This enables gap analyses, prioritizations of Earth observations, and discovery of products and services meeting the information needs. "What if?" questions supports knowledge creation supporting the development of policies and activities to make progress towards the SDGs. Increasingly, user types, applications and requirements are linked to actual persons, models and datasets, respectively, and this allows both the social networking of providers and users and the execution of business processes. A core function of the SEE-IN KB is to facilitate the linkage of societal goals, targets, and indicators to EVs that need to be monitored in order to measure progress towards the targets. Applying a goal-based approach used to identify the EVs to the SDG Indicators revealed that some SDG Indicators require traditional Earth observations for quantification, while many of the EVs are related to the built environment. For many of the SDG Indicators, integration of socio-economic statistical data with environmental data, including in situ observations, is of importance. The goal-based approach was also applied to the SDG Targets, and this analysis showed that many of the Targets would benefit from

  20. Fast discriminative latent Dirichlet allocation

    Data.gov (United States)

    National Aeronautics and Space Administration — This is the code for fast discriminative latent Dirichlet allocation, which is an algorithm for topic modeling and text classification. The related paper is at...

  1. Fast Picometer Mirror Mount Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is a 6DOF controllable mirror mount with high dynamic range and fast tip/tilt capability for space based applications. It will enable the...

  2. Fast generation of dendritic cells

    DEFF Research Database (Denmark)

    Kvistborg, P; Bøgh, Marie; Claesson, M H

    2009-01-01

    we have developed fast DC protocol by comparing two different fast DC protocols with SDDC. DC were evaluated by FACS analysis, and the optimal profile was considered: CD14(low), CD80(high), CD83(high), CD86(high), CCR7(high), HLA class I and II(high). FACS profiles were used as the selection criteria...... together with yield and morphology. Two fast DC protocols fulfilled these criteria and were selected for functional analysis. Our results demonstrate that DC generated within 5days or 48h are comparable with SDDC both phenotypically and functionally. However, we found that 48h DC were more susceptible than...... SDDC to the IL-10 inducing stimulus of TLR ligands (R848 and LPS). Thus to determine the clinical relevance of fast DC protocols in cancer settings, small phase I trials should be conducted monitoring regulatory T cells carefully....

  3. Still on the Fast Track

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    North China’s Inner Mongolia Autonomous Region’s development is progressing comparatively fast,though there is still a long way to go until it catches up with advances being made in other parts of China, officials said.

  4. vysmaw: Fast visibility stream muncher

    Science.gov (United States)

    Pokorny, Martin; Law, Casey J.

    2017-10-01

    The vysmaw client library facilitates the development of code for processes to tap into the fast visibility stream on the National Radio Astronomy Observatory's Very Large Array correlator back-end InfiniBand network.

  5. Allegheny County Fast Food Establishments

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Allegheny County Health Department has generated this list of fast food restaurants by exporting all chain restaurants without an alcohol permit from the...

  6. 27 CFR 9.167 - Red Mountain

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Red Mountain 9.167 Section... Mountain (a) Name. The name of the viticultural area described in this section is “Red Mountain.” (b) Approved maps. The appropriate map for determining the boundaries of the Red Mountain viticultural area...

  7. Fast Calorimeter Simulation in ATLAS

    CERN Document Server

    Schaarschmidt, Jana; The ATLAS collaboration

    2017-01-01

    Producing the very large samples of simulated events required by many physics and performance studies with the ATLAS detector using the full GEANT4 detector simulation is highly CPU intensive. Fast simulation tools are a useful way of reducing CPU requirements when detailed detector simulations are not needed. During the LHC Run-1, a fast calorimeter simulation (FastCaloSim) was successfully used in ATLAS. FastCaloSim provides a simulation of the particle energy response at the calorimeter read-out cell level, taking into account the detailed particle shower shapes and the correlations between the energy depositions in the various calorimeter layers. It is interfaced to the standard ATLAS digitization and reconstruction software, and it can be tuned to data more easily than GEANT4. It is 500 times faster than full simulation in the calorimeter system. Now an improved version of FastCaloSim is in development, incorporating the experience with the version used during Run-1. The new FastCaloSim makes use of mach...

  8. Upgrading ATLAS Fast Calorimeter Simulation

    CERN Document Server

    Heath, Matthew Peter; The ATLAS collaboration

    2017-01-01

    Producing the very large samples of simulated events required by many physics and performance studies with the ATLAS detector using the full GEANT4 detector simulation is highly CPU intensive. Fast simulation tools are a useful way of reducing CPU requirements when detailed detector simulations are not needed. During the LHC Run-1, a fast calorimeter simulation (FastCaloSim) was successfully used in ATLAS. FastCaloSim provides a simulation of the particle energy response at the calorimeter read-out cell level, taking into account the detailed particle shower shapes and the correlations between the energy depositions in the various calorimeter layers. It is interfaced to the standard ATLAS digitization and reconstruction software, and it can be tuned to data more easily than Geant4. Now an improved version of FastCaloSim is in development, incorporating the experience with the version used during Run-1. The new FastCaloSim aims to overcome some limitations of the first version by improving the description of s...

  9. Quantifying Cricket Fast Bowling Skill.

    Science.gov (United States)

    Feros, Simon A; Young, Warren B; O'Brien, Brendan J

    2017-09-27

    To evaluate the current evidence regarding the quantification of cricket fast bowling skill. Studies that assessed fast bowling skill (bowling speed and accuracy) were identified from searches in SPORTDiscus (EBSCO) in June 2017. The reference lists of identified papers were also examined for relevant investigations. Sixteen papers matched the inclusion criteria, and discrepancies in assessment procedures were evident. Differences in: test environment, pitch and cricket ball characteristics, the warm-up prior to test, test familiarisation procedures, permitted run-up lengths, bowling spell length, delivery sequence, test instructions, collection of bowling speed data, collection and reportage of bowling accuracy data were apparent throughout the literature. The reliability and sensitivity of fast bowling skill measures has rarely been reported across the literature. Only one study has attempted to assess the construct validity of their skill measures. There are several discrepancies in how fast bowling skill has been assessed and subsequently quantified in the literature to date. This is a problem, as comparisons between studies are often difficult. Therefore, a strong rationale exists for the creation of match-specific standardised fast bowling assessments that offer greater ecological validity while maintaining acceptable reliability and sensitivity of the skill measures. If prospective research can act on the proposed recommendations from this review, then coaches will be able to make more informed decisions surrounding player selection, talent identification, return to skill following injury, and the efficacy of short- and long-term training interventions for fast bowlers.

  10. Red nodule on the breast

    Directory of Open Access Journals (Sweden)

    Roberta Colucci

    2012-01-01

    Full Text Available A 63-year-old woman living in the countryside referred to our department with a 2-month history of a red nodule localized on the right breast. Histological examination, immunohistochemical analyses and serologic evaluation conducted with ELISA and Western blot were performed. Clinical diagnosis of borrelial lymphocytoma was not possible solely on the clinical presentation of a classical nodular form without lymphoadenopathy. An absence of a referred prior tick bite and a previous or concomitant erythema migrans at clinical presentation rendered a more challenging diagnosis. The fact that the patient lived in the countryside, the appearance of the breast nodule in September, and serologic, histologic, and immunohistochemical analysis facilitated the diagnosis of borrelial lymphocytoma. We report this case to highlight the importance of an investigation of Lyme borreliosis when a patient living in the countryside presents with a red nodule of the nipple and areola.

  11. Extremely red quasars in BOSS

    Science.gov (United States)

    Hamann, Fred; Zakamska, Nadia L.; Ross, Nicholas; Paris, Isabelle; Alexandroff, Rachael M.; Villforth, Carolin; Richards, Gordon T.; Herbst, Hanna; Brandt, W. Niel; Cook, Ben; Denney, Kelly D.; Greene, Jenny E.; Schneider, Donald P.; Strauss, Michael A.

    2017-01-01

    Red quasars are candidate young objects in an early transition stage of massive galaxy evolution. Our team recently discovered a population of extremely red quasars (ERQs) in the Baryon Oscillation Spectroscopic Survey (BOSS) that has a suite of peculiar emission-line properties including large rest equivalent widths (REWs), unusual `wingless' line profiles, large N V/Lyα, N V/C IV, Si IV/C IV and other flux ratios, and very broad and blueshifted [O III] λ5007. Here we present a new catalogue of C IV and N V emission-line data for 216 188 BOSS quasars to characterize the ERQ line properties further. We show that they depend sharply on UV-to-mid-IR colour, secondarily on REW(C IV), and not at all on luminosity or the Baldwin Effect. We identify a `core' sample of 97 ERQs with nearly uniform peculiar properties selected via i-W3 ≥ 4.6 (AB) and REW(C IV) ≥ 100 Å at redshifts 2.0-3.4. A broader search finds 235 more red quasars with similar unusual characteristics. The core ERQs have median luminosity ˜ 47.1, sky density 0.010 deg-2, surprisingly flat/blue UV spectra given their red UV-to-mid-IR colours, and common outflow signatures including BALs or BAL-like features and large C IV emission-line blueshifts. Their SEDs and line properties are inconsistent with normal quasars behind a dust reddening screen. We argue that the core ERQs are a unique obscured quasar population with extreme physical conditions related to powerful outflows across the line-forming regions. Patchy obscuration by small dusty clouds could produce the observed UV extinctions without substantial UV reddening.

  12. Red fox sightings in Rome

    Directory of Open Access Journals (Sweden)

    Bruno Cignini

    1997-12-01

    Full Text Available Abstract In this study preliminary data on the presence of Red fox in Rome (an area of 360 km² within the Rome ringroad. G.R.A. since 1980 are presented. The data were mapped on a UTM 1 sq. km. grid. Data were analysed and correlated, for each City district, with the prevalent environment (green, built-up, river-side areas and with the density of inhabitants.

  13. Biobleaching of Acacia kraft pulp with extracellular enzymes secreted by Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 using low-cost media.

    Science.gov (United States)

    Afrida, Sitompul; Tamai, Yutaka; Watanabe, Toshihiro; Osaki, Mitsuru

    2014-08-01

    The white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 have been shown in previous studies to have high biobleaching activity in vivo. The aim of this study was to investigate the activities and stabilities of extracellular enzymes, prepared from I. lacteus and L. tigrinus culture grown in three types of economical media of agricultural and forestry wastes, for biobleaching of Acacia oxygen-delignified kraft pulp using kappa number reduction as an indicator of delignification. After 3 days of incubation, the extracellular enzymes preparations from I. lacteus and L. tigrinus cultures in media of Acacia mangium wood powder supplemented with rice bran and addition 1 % glucose (WRBG), resulted in significant decrease of 4.4 and 6.7 %, respectively. A slightly higher kappa number reduction (7.4 %) was achieved with the combine extracellular enzymes from I. lacteus and L. tigrinus. One of the strategies for reducing the cost of enzyme production for treatment processes in the pulp and paper industry is the utilization of agricultural and forestry waste. Thus, WRBG has potential as a culture medium for producing stable lignolytic enzymes simply and economically.

  14. Genetic and physical fine mapping of the novel brown midrib gene bm6 in maize (Zea mays L.) to a 180 kb region on chromosome 2.

    Science.gov (United States)

    Chen, Yongsheng; Liu, Hongjun; Ali, Farhad; Scott, M Paul; Ji, Qing; Frei, Ursula Karoline; Lübberstedt, Thomas

    2012-10-01

    Brown midrib mutants in maize are known to be associated with reduced lignin content and increased cell wall digestibility, which leads to better forage quality and higher efficiency of cellulosic biomass conversion into ethanol. Four well known brown midrib (bm) mutants, named bm1-4, were identified several decades ago. Additional recessive brown midrib mutants have been identified by allelism tests and designated as bm5 and bm6. In this study, we determined that bm6 increases cell wall digestibility and decreases plant height. bm6 was confirmed onto the short arm of chromosome 2 by a small mapping set with 181 plants from a F(2) segregating population, derived from crossing B73 and a bm6 mutant line. Subsequently, 960 brown midrib individuals were selected from the same but larger F(2) population for genetic and physical mapping. With newly developed markers in the target region, the bm6 gene was assigned to a 180 kb interval flanked by markers SSR_308337 and SSR_488638. In this region, ten gene models are predicted in the maize B73 sequence. Analysis of these ten genes as well as genes in the syntenic rice region revealed that four of them are promising candidate genes for bm6. Our study will facilitate isolation of the underlying gene of bm6 and advance our understanding of brown midrib gene functions.

  15. Identities of P2 and P3 Residues of H-2Kb-Bound Peptides Determine Mouse Ly49C Recognition.

    Directory of Open Access Journals (Sweden)

    Elsa A Marquez

    Full Text Available Ly49 receptors can be peptide selective in their recognition of MHC-I-peptide complexes, affording them a level of discrimination beyond detecting the presence or absence of specific MHC-I allele products. Despite this ability, little is understood regarding the properties that enable some peptides, when bound to MHC-I molecules, to support Ly49 recognition, but not others. Using RMA-S target cells expressing MHC-I molecules loaded with individual peptides and effector cells expressing the ectodomain of the inhibitory Ly49C receptor, we found that two adjacent amino acid residues, P2 and P3, both buried in the peptide binding groove of H-2Kb, determine mouse Ly49C specificity. If both are aliphatic residues, this is supportive. Whereas, small amino acids at P2 and aromatic amino acids at the P3 auxiliary anchor residue are detrimental to Ly49C recognition. These results resemble those with a rat Ly49 where the identity of a peptide anchor residue determines recognition, suggesting that dependence on specific peptide residues buried in the MHC-I peptide-binding groove may be fundamental to Ly49 peptide selectivity and recognition.

  16. Fifty Years in the Development of a Glutaminergic-Dopaminergic Optimization Complex (KB220) to Balance Brain Reward Circuitry in Reward Deficiency Syndrome: A Pictorial

    Science.gov (United States)

    Blum, K; Febo, M; Badgaiyan, RD

    2016-01-01

    Dopamine along with other chemical messengers like serotonin, cannabinoids, endorphins and glutamine, play significant roles in brain reward processing. There is a devastating opiate/opioid epidemicin the United States. According to the Centers for Disease Control and Prevention (CDC), at least 127 people, young and old, are dying every day due to narcotic overdose and alarmingly heroin overdose is on the rise. The Food and Drug Administration (FDA) has approved some Medication-Assisted Treatments (MATs) for alcoholism, opiate and nicotine dependence, but nothing for psychostimulant and cannabis abuse. While these pharmaceuticals are essential for the short-term induction of “psychological extinction,” in the long-term caution is necessary because their use favors blocking dopaminergic function indispensable for achieving normal satisfaction in life. The two institutions devoted to alcoholism and drug dependence (NIAAA & NIDA) realize that MATs are not optimal and continue to seek better treatment options. We review, herein, the history of the development of a glutaminergic-dopaminergic optimization complex called KB220 to provide for the possible eventual balancing of the brain reward system and the induction of “dopamine homeostasis.” This complex may provide substantial clinical benefit to the victims of Reward Deficiency Syndrome (RDS) and assist in recovery from iatrogenically induced addiction to unwanted opiates/opioids and other addictive behaviors. PMID:27840857

  17. The Hypocrea jecorina (Trichoderma reesei hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding region of the wild-type genome

    Directory of Open Access Journals (Sweden)

    Hartl Lukas

    2008-07-01

    Full Text Available Abstract Background The hypercellulolytic mutant Hypocrea jecorina (anamorph Trichoderma reesei RUT C30 is the H. jecorina strain most frequently used for cellulase fermentations and has also often been employed for basic research on cellulase regulation. This strain has been reported to contain a truncated carbon catabolite repressor gene cre1 and is consequently carbon catabolite derepressed. To date this and an additional frame-shift mutation in the glycoprotein-processing β-glucosidase II encoding gene are the only known genetic differences in strain RUT C30. Results In the present paper we show that H. jecorina RUT C30 lacks an 85 kb genomic fragment, and consequently misses additional 29 genes comprising transcription factors, enzymes of the primary metabolism and transport proteins. This loss is already present in the ancestor of RUT C30 – NG 14 – and seems to have occurred in a palindromic AT-rich repeat (PATRR typically inducing chromosomal translocations, and is not linked to the cre1 locus. The mutation of the cre1 locus has specifically occurred in RUT C30. Some of the genes that are lacking in RUT C30 could be correlated with pronounced alterations in its phenotype, such as poor growth on α-linked oligo- and polyglucosides (loss of maltose permease, or disturbance of osmotic homeostasis. Conclusion Our data place a general caveat on the use of H. jecorina RUT C30 for further basic research.

  18. A 590 kb deletion caused by non-allelic homologous recombination between two LINE-1 elements in a patient with mesomelia-synostosis syndrome.

    Science.gov (United States)

    Kohmoto, Tomohiro; Naruto, Takuya; Watanabe, Miki; Fujita, Yuji; Ujiro, Sae; Okamoto, Nana; Horikawa, Hideaki; Masuda, Kiyoshi; Imoto, Issei

    2017-04-01

    Mesomelia-synostoses syndrome (MSS) is a rare, autosomal-dominant, syndromal osteochondrodysplasia characterized by mesomelic limb shortening, acral synostoses, and multiple congenital malformations due to a non-recurrent deletion at 8q13 that always encompasses two coding-genes, SULF1 and SLCO5A1. To date, five unrelated patients have been reported worldwide, and MMS was previously proposed to not be a genomic disorder associated with deletions recurring from non-allelic homologous recombination (NAHR) in at least two analyzed cases. We conducted targeted gene panel sequencing and subsequent array-based copy number analysis in an 11-year-old undiagnosed Japanese female patient with multiple congenital anomalies that included mesomelic limb shortening and detected a novel 590 Kb deletion at 8q13 encompassing the same gene set as reported previously, resulting in the diagnosis of MSS. Breakpoint sequences of the deleted region in our case demonstrated the first LINE-1s (L1s)-mediated unequal NAHR event utilizing two distant L1 elements as homology substrates in this disease, which may represent a novel causative mechanism of the 8q13 deletion, expanding the range of mechanisms involved in the chromosomal rearrangements responsible for MSS.

  19. Whole Genome Sequencing Identifies a 78 kb Insertion from Chromosome 8 as the Cause of Charcot-Marie-Tooth Neuropathy CMTX3

    Science.gov (United States)

    Brewer, Megan H.; Chaudhry, Rabia; Qi, Jessica; Kidambi, Aditi; Drew, Alexander P.; Ryan, Monique M.; Subramanian, Gopinath M.; Young, Helen K.; Zuchner, Stephan; Reddel, Stephen W.; Nicholson, Garth A.; Kennerson, Marina L.

    2016-01-01

    With the advent of whole exome sequencing, cases where no pathogenic coding mutations can be found are increasingly being observed in many diseases. In two large, distantly-related families that mapped to the Charcot-Marie-Tooth neuropathy CMTX3 locus at chromosome Xq26.3-q27.3, all coding mutations were excluded. Using whole genome sequencing we found a large DNA interchromosomal insertion within the CMTX3 locus. The 78 kb insertion originates from chromosome 8q24.3, segregates fully with the disease in the two families, and is absent from the general population as well as 627 neurologically normal chromosomes from in-house controls. Large insertions into chromosome Xq27.1 are known to cause a range of diseases and this is the first neuropathy phenotype caused by an interchromosomal insertion at this locus. The CMTX3 insertion represents an understudied pathogenic structural variation mechanism for inherited peripheral neuropathies. Our finding highlights the importance of considering all structural variation types when studying unsolved inherited peripheral neuropathy cases with no pathogenic coding mutations. PMID:27438001

  20. Characterization of an Equine α-S2-Casein Variant Due to a 1.3 kb Deletion Spanning Two Coding Exons.

    Directory of Open Access Journals (Sweden)

    Julia Brinkmann

    Full Text Available The production and consumption of mare's milk in Europe has gained importance, mainly based on positive health effects and a lower allergenic potential as compared to cows' milk. The allergenicity of milk is to a certain extent affected by different genetic variants. In classical dairy species, much research has been conducted into the genetic variability of milk proteins, but the knowledge in horses is scarce. Here, we characterize two major forms of equine αS2-casein arising from genomic 1.3 kb in-frame deletion involving two coding exons, one of which represents an equid specific duplication. Findings at the DNA-level have been verified by cDNA sequencing from horse milk of mares with different genotypes. At the protein-level, we were able to show by SDS-page and in-gel digestion with subsequent LC-MS analysis that both proteins are actually expressed. The comparison with published sequences of other equids revealed that the deletion has probably occurred before the ancestor of present-day asses and zebras diverged from the horse lineage.

  1. A 600 kb triplication in the cat eye syndrome critical region causes anorectal, renal and preauricular anomalies in a three-generation family.

    Science.gov (United States)

    Knijnenburg, Jeroen; van Bever, Yolande; Hulsman, Lorette O M; van Kempen, Chantal A P; Bolman, Galhana M; van Loon, Rosa Laura E; Beverloo, H Berna; van Zutven, Laura J C M

    2012-09-01

    Cat eye syndrome (CES) is caused by a gain of the proximal part of chromosome 22. Usually, a supernumerary marker chromosome is present, containing two extra copies of the chromosome 22q11.1q11.21 region. More sporadically, the gain is present intrachromosomally. The critical region for CES is currently estimated to be about 2.1 Mb and to contain at least 14 RefSeq genes. Gain of this region may cause ocular coloboma, preauricular, anorectal, urogenital and congenital heart malformations. We describe a family in which a 600 kb intrachromosomal triplication is present in at least three generations. The copy number alteration was detected using MLPA and further characterized with interphase and metaphase FISH and SNP-array. The amplified fragment is located in the distal part of the CES region. The family members show anal atresia and preauricular tags or pits, matching part of the phenotype of this syndrome. This finding suggests that amplification of the genes CECR2, SLC25A18 and ATP6V1E1, mapping within the critical region for CES, may be responsible for anorectal, renal and preauricular anomalies in patients with CES.

  2. Growth, Optical, Mechanical, Dielectric and Theoretical Studies on Potassium Pentaborate Tetrahydrate (KB_5O_8·4H_2O) Single Crystal by Modified Sankaranarayanan-Ramasamy Method

    Institute of Scientific and Technical Information of China (English)

    C. Justin Raj; S. Krishnan; S. Dinakaran; J. Mary Linet; R. Uthrakumar; R. Robert; Jerome Das

    2009-01-01

    A nonlinear optical single crystal of potassium pentaborate tetrahydrate (KB_5O_8·4H_2O) has been grown from aqueous solution by using unidirectional crystal growth method of Sankaranarayanan-Ramasamy (SR) with a due modification in the growth assembly. Potassium pentaborate crystal of 60 mm length and 10 mm diameter has been grown along (100) plane with a growth rate of 3 mm per day within a period of 20 days. The grown crystal was subjected to single crystal X-ray diffraction analysis to confirm that the crystal belongs to the orthorhombic system. Some fundamental data such as valance electron plasma energy, Penn gap, Fermi energy and electronic polarizability of the grown crystal were calculated. The presence of borate in the grown crystal was confirmed by Fourier transform infrared (FTIR) spectroscopy. The optical transmission property of the grown crystal was analyzed using ultra violet (UV) visible spectral analysis. Surface morphology of the growth plane was observed using scanning electron microscopy (SEM). The mechanical strength of the crystals was found out using Vickers microhardness test along the growth axis. Frequency dependent dielectric constant of the grown crystal was studied for various temperatures along (100) plane.

  3. Theory of Fast Electron Transport for Fast Ignition

    CERN Document Server

    Robinson, A P L; Davies, J R; Gremillet, L; Honrubia, J J; Johzaki, T; Kingham, R J; Sherlock, M; Solodov, A A

    2013-01-01

    Fast Ignition Inertial Confinement Fusion is a variant of inertial fusion in which DT fuel is first compressed to high density and then ignited by a relativistic electron beam generated by a fast (< 20 ps) ultra-intense laser pulse, which is usually brought in to the dense plasma via the inclusion of a re-entrant cone. The transport of this beam from the cone apex into the dense fuel is a critical part of this scheme, as it can strongly influence the overall energetics. Here we review progress in the theory and numerical simulation of fast electron transport in the context of Fast Ignition. Important aspects of the basic plasma physics, descriptions of the numerical methods used, a review of ignition-scale simulations, and a survey of schemes for controlling the propagation of fast electrons are included. Considerable progress has taken place in this area, but the development of a robust, high-gain FI `point design' is still an ongoing challenge.

  4. Extremely Red Quasars in BOSS

    CERN Document Server

    Hamann, Fred; Ross, Nicholas; Paris, Isabelle; Alexandroff, Rachael M; Villforth, Carolin; Richards, Gordon T; Herbst, Hanna; Brandt, W Niel; Cook, Ben; Denney, Kelly D; Greene, Jenny E; Schneider, Donald P; Strauss, Michael A

    2016-01-01

    Red quasars are candidate young objects in an early transition stage of massive galaxy evolution. Our team recently discovered a population of extremely red quasars (ERQs) in the Baryon Oscillation Spectroscopic Survey (BOSS) that has a suite of peculiar emission-line properties including large rest equivalent widths (REWs), unusual "wingless" line profiles, large NV/Lya, NV/CIV, SiIV/CIV and other flux ratios, and very broad and blueshifted [OIII] 5007. Here we present a new catalog of CIV and NV emission-line data for 216,188 BOSS quasars to characterize the ERQ line properties further. We show that they depend sharply on UV-to-mid-IR color, secondarily on REW(CIV), and not at all on luminosity or the Baldwin Effect. We identify a "core" sample of 97 ERQs with nearly uniform peculiar properties selected via i-W3 > 4.6 (AB) and REW(CIV) > 100 A at redshifts 2.0-3.4. A broader search finds 235 more red quasars with similar unusual characteristics. The core ERQs have median luminosity log L (ergs/s) ~ 47.1, sk...

  5. Red cell DAMPs and inflammation.

    Science.gov (United States)

    Mendonça, Rafaela; Silveira, Angélica A A; Conran, Nicola

    2016-09-01

    Intravascular hemolysis, or the destruction of red blood cells in the circulation, can occur in numerous diseases, including the acquired hemolytic anemias, sickle cell disease and β-thalassemia, as well as during some transfusion reactions, preeclampsia and infections, such as those caused by malaria or Clostridium perfringens. Hemolysis results in the release of large quantities of red cell damage-associated molecular patterns (DAMPs) into the circulation, which, if not neutralized by innate protective mechanisms, have the potential to activate multiple inflammatory pathways. One of the major red cell DAMPs, heme, is able to activate converging inflammatory pathways, such as toll-like receptor signaling, neutrophil extracellular trap formation and inflammasome formation, suggesting that this DAMP both activates and amplifies inflammation. Other potent DAMPs that may be released by the erythrocytes upon their rupture include heat shock proteins (Hsp), such as Hsp70, interleukin-33 and Adenosine 5' triphosphate. As such, hemolysis represents a major inflammatory mechanism that potentially contributes to the clinical manifestations that have been associated with the hemolytic diseases, such as pulmonary hypertension and leg ulcers, and likely plays a role in specific complications of sickle cell disease such as endothelial activation, vaso-occlusive processes and tissue injury.

  6. Impact of Short-Term Intake of Red Wine and Grape Polyphenol Extract on the Human Metabolome

    NARCIS (Netherlands)

    Jacobs, D.M.; Fuhrmann, J.C.; Dorsten, van F.A.; Rein, D.; Peters, S.; Velzen, van E.J.J.; Hollebrands, B.; Draijer, R.; Duynhoven, van J.P.M.; Garczarek, U.

    2012-01-01

    Red wine and grape polyphenols are considered to promote cardiovascular health and are involved in multiple biological functions. Their overall impact on the human metabolome is not known. Therefore, exogenous and endogenous metabolic effects were determined in fasting plasma and 24 h urine from hea

  7. Development and improvement of measuring method for growth rate of intracellular symbiotic acid-fast bacteria using radioisotopes

    Energy Technology Data Exchange (ETDEWEB)

    Nakata, Noboru; Fukutomi, Yasuo [National Inst. for Leprosy Research, Higashimurayama, Tokyo (Japan)

    1997-02-01

    The aim of this research group was to investigate the factors which might mediate the growth of mycobacterium lepra and relate to its affinity to the nerve tissue. In this year, constructions of a mycobacterium smegmatis mutant having a high transform ability and a shuttle vector between E. coli and acid-fast bacteria was attempted. From the wild type of m. smegmatis, a highly transformable mutant was obtained and the rate of transformation of the mutant was ca. 10{sup 5} times higher than the parent. And two shuttle vectors for E. coli/acid-fast bacteria; pALKMZErO (6.2 kb) and pHSGM59 (5.4 kb) were constructed. Since the former was unstable in M. smegmatis, the latter vector was used for the following experiments. Expression of `cat` gene cloned by pHSGM59 was identified in M. smegmatis. Further, DNA library of M. leprae was prepared by the use of the vector. Approximately, 1 x 10{sup 4} transformed clones were obtained. The analysis of the plasmids recovered from the clones is under way. (M.N.)

  8. Exercise attenuates the fasting-induced transcriptional activation of metabolic genes in skeletal muscle.

    Science.gov (United States)

    Hildebrandt, A L; Neufer, P D

    2000-06-01

    Fasting elicits a progressive increase in lipid metabolism within skeletal muscle. To determine the effects of fasting on the transcriptional regulation of genes important for metabolic control in skeletal muscle composed of different fiber types, nuclei from control and fasted (24 and 72 h) rats were subjected to nuclear run-on analysis using an RT-PCR-based technique. Fasting increased (P < 0.05) transcription rate of the muscle-specific uncoupling protein-3 gene (UCP3) 14.3- to 21.1-fold in white gastrocnemius (WG; fast-twitch glycolytic) and 5.5- to 7.5-fold in red gastrocnemius (RG; fast-twitch oxidative) and plantaris (PL; mixed) muscles. No change occurred in soleus (slow-twitch oxidative) muscle. Fasting also increased transcription rate of the lipoprotein lipase (LPL), muscle carnitine palmitoyltransferase I (CPT I), and long-chain acyl-CoA dehydrogenase (LCAD) genes 1.7- to 3.7-fold in WG, RG, and PL muscles. Transcription rate responses were similar after 24 and 72 h of fasting. Surprisingly, increasing metabolic demand during the initial 8 h of starvation (two 2-h bouts of treadmill running) attenuated the 24-h fasting-induced transcriptional activation of UCP3, LPL, CPT I, and LCAD in RG and PL muscles, suggesting the presence of opposing regulatory mechanisms. These data demonstrate that fasting elicits a fiber type-specific coordinate increase in the transcription rate of several genes involved in and/or required for lipid metabolism and indicate that exercise may attenuate the fasting-induced transcriptional activation of specific metabolic genes.

  9. Genomic sequence analysis of the 238-kb swine segment with a cluster of TRIM and olfactory receptor genes located, but with no class I genes, at the distal end of the SLA class I region.

    Science.gov (United States)

    Ando, Asako; Shigenari, Atsuko; Kulski, Jerzy K; Renard, Christine; Chardon, Patrick; Shiina, Takashi; Inoko, Hidetoshi

    2005-12-01

    Continuous genomic sequence has been previously determined for the swine leukocyte antigen (SLA) class I region from the TNF gene cluster at the border between the major histocompatibility complex (MHC) class III and class I regions to the UBD gene at the telomeric end of the classical class I gene cluster (SLA-1 to SLA-5, SLA-9, SLA-11). To complete the genomic sequence of the entire SLA class I genomic region, we have analyzed the genomic sequences of two BAC clones carrying a continuous 237,633-bp-long segment spanning from the TRIM15 gene to the UBD gene located on the telomeric side of the classical SLA class I gene cluster. Fifteen non-class I genes, including the zinc finger and the tripartite motif (TRIM) ring-finger-related family genes and olfactory receptor genes, were identified in the 238-kilobase (kb) segment, and their location in the segment was similar to their apparent human homologs. In contrast, a human segment (alpha block) spanning about 375 kb from the gene ETF1P1 and from the HLA-J to HLA-F genes was absent from the 238-kb swine segment. We conclude that the gene organization of the MHC non-class I genes located in the telomeric side of the classical SLA class I gene cluster is remarkably similar between the swine and the human segments, although the swine lacks a 375-kb segment corresponding to the human alpha block.

  10. A 350-kb cosmid contig in 3p14.2 that crosses the t(3;8) hereditary renal cell carcinoma translocation breakpoint and 17 aphidicolin-induced FRA3B breakpoints

    Energy Technology Data Exchange (ETDEWEB)

    Paradee, W. [Wayne State Univ. School of Medicine, Detroit, MI (United States); Wilke, C.M.; Hoge, A. [Univ. of Michigan Medical School, Ann Arbor, MI (United States)] [and others

    1996-07-11

    The constitutive fragile site at human chromosomal band 3p14.2, FRA3B, has been described as the most active common fragile site in the human genome. Previous work demonstrated that a 1330-kb YAC clone, YC850A6, spans both the t(3;8) translocation and FRA3B and also encompasses FRA3B-associated breakpoints was used to construct a multi-hit cosmid library. Screening of this library resulted in a 350-kb cosmid contig that extends distally from the t(3;8) translocation breakpoint. Seventeen aphidicolin-induced 3p14.2 breakpoints derived from hamster-human hybrids were mapped within this cosmid contig. These breakpoints were found to localize as two distinct clusters, separated by 200 kb, which lie on either side of a region of frequent breakage within FRA3B as defined by FISH analysis using cosmids from the contigs. The distribution of these breakpoints, together with the region of frequent chromosomal breakage mapped by FISH analysis, further confirms the position of FRA3B comprises several hundred kilobases of DNA sequence within 3p14.2. The 350-kb contig and the cosmid library constructed from YAC YC850A6 will be essential for further characterization of the region surrounding FRA3B and in experiments to determine the molecular basis of the fragility of FRA3B.

  11. Localization and physical mapping of a plasmid-borne 23-kb nif gene cluster from Enterobacter agglomerans showing homology to the entire nif gene cluster of Klebsiella pneumoniae M5a1.

    Science.gov (United States)

    Singh, M; Kreutzer, R; Acker, G; Klingmüller, W

    1988-01-01

    A physical and genetical map of the plasmid pEA3 indigenous to Enterobacter agglomerans is presented. pEA3 is a 111-kb large plasmid containing a 23-kb large cluster of nif genes which shows extensive homology (Southern hybridization and heteroduplex analysis) to the entire nif gene cluster of Klebsiella pneumoniae (Kp) M5a1. All the nif genes on pEA3 are organized in the same manner as in K. pneumoniae, except nifJ, which is located on the left end of pEA3 nif gene cluster (near nifQB). A BamHI restriction map of pEA3 and a detailed restriction map of the 23-kb nif region on pEA3 is also presented. The nif genes of pEA3 showed a low level of acetylene reduction in Escherichia coli, demonstrating that these genes are functional and contain the whole genetic information required to fix nitrogen. The origin of vegetative replication (OriV) of pEA3 was localized about 5.5 kb from the right end of the nif gene cluster. In addition to pEA3, large plasmids from four other strains of E. agglomerans showed homology to all the Kp nif genes tested, indicating that in diazotrophic strains of E. agglomerans nif genes are usually located on plasmids. In contrast, in most of the free-living, nitrogen-fixing bacteria the nif genes are on chromosome.

  12. Prevalence of βS-globin gene haplotypes, α-thalassemia (3.7 kb deletion and redox status in patients with sickle cell anemia in the state of Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Eliana LitsukoTomimatsu Shimauti

    2015-09-01

    Full Text Available The aim of this study was to determine the frequency of beta S-globin gene (βS globin haplotypes and alpha thalassemia with 3.7 kb deletion (−α3.7kb thalassemia in the northwest region of Paraná state, and to investigate the oxidative and clinical-hematological profile of βS globin carriers in this population. Of the 77 samples analyzed, 17 were Hb SS, 30 were Hb AS and 30 were Hb AA. The βSglobin haplotypes and −α3.7kb thalassemia were identified using polymerase chain reaction.Trolox equivalent antioxidant capacity (TEAC and lipid peroxidation (LPO were assessed spectophotometrically. Serum melatonin levels were determined using high-performance liquid chromatography coupled to coulometric electrochemical detection. The haplotype frequencies in the SS individuals were as follows: Bantu- 21 (62%, Benin - 11 (32% and Atypical- 2 (6%. Bantu/Benin was the most frequent genotype. Of the 47 SS and AS individuals assessed, 17% (n = 8 had the −α3.7kb mutation. Clinical manifestations, as well as serum melatonin, TEAC and LPO levels did not differ between Bantu/Bantu and Bantu/Benin individuals (p > 0.05. Both genotypes were associated with high LPO and TEAC levels and decreased melatonin concentration. These data suggest that the level of oxidative stress in patients with Bantu/Bantu and Bantu/Benin genotypes may overload the antioxidant capacity.

  13. Prevalence of β(S)-globin gene haplotypes, α-thalassemia (3.7 kb deletion) and redox status in patients with sickle cell anemia in the state of Paraná, Brazil.

    Science.gov (United States)

    Shimauti, Eliana LitsukoTomimatsu; Silva, Danilo Grunig Humberto; de Souza, Eniuce Menezes; de Almeida, Eduardo Alves; Leal, Francismar Prestes; Bonini-Domingos, Claudia Regina

    2015-01-01

    The aim of this study was to determine the frequency of beta S-globin gene (β(S) globin) haplotypes and alpha thalassemia with 3.7 kb deletion (-α(3.7kb) thalassemia) in the northwest region of Paraná state, and to investigate the oxidative and clinical-hematological profile of β(S) globin carriers in this population. Of the 77 samples analyzed, 17 were Hb SS, 30 were Hb AS and 30 were Hb AA. The β(S)globin haplotypes and -α(3.7kb) thalassemia were identified using polymerase chain reaction.Trolox equivalent antioxidant capacity (TEAC) and lipid peroxidation (LPO) were assessed spectophotometrically. Serum melatonin levels were determined using high-performance liquid chromatography coupled to coulometric electrochemical detection. The haplotype frequencies in the SS individuals were as follows: Bantu- 21 (62%), Benin - 11 (32%) and Atypical- 2 (6%). Bantu/Benin was the most frequent genotype. Of the 47 SS and AS individuals assessed, 17% (n = 8) had the -α(3.7kb) mutation. Clinical manifestations, as well as serum melatonin, TEAC and LPO levels did not differ between Bantu/Bantu and Bantu/Benin individuals (p > 0.05). Both genotypes were associated with high LPO and TEAC levels and decreased melatonin concentration. These data suggest that the level of oxidative stress in patients with Bantu/Bantu and Bantu/Benin genotypes may overload the antioxidant capacity.

  14. CHARACTERIZATION OF A 3.3-KB PLASMID OF ESCHERICHIA COLI O157:H7 AND EVALUATION OF STABILITY OF GENETICALLY ENGINEERED DERIVATIVES OF THIS PLASMID EXPRESSING GREEN FLUORESCENCE

    Science.gov (United States)

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 (strain 86-24) harbors a 3.3 kb, cryptic plasmid (pSP70) that does not encode a selectable phenotype. A transposon (Tn) encoding kanamycin resistance (Kan**r) was inserted by in vitro transposon mutagenesis at a random location on pSP70 to construct...

  15. A Comparison of Fast Marching, Fast Sweeping and Fast Iterative Methods for the Solution of the Eikonal Equation

    Directory of Open Access Journals (Sweden)

    A. Capozzoli

    2014-11-01

    Full Text Available We compare the computational performance of the Fast Marching Method, the Fast Sweeping Method and the Fast Iterative Method to determine a numerical solution to the eikonal equation. We point out how the Fast Iterative Method outperforms the other two thanks to its parallel processing capabilities.

  16. Preoperative fasting time in children.

    LENUS (Irish Health Repository)

    Adeel, S

    2012-02-01

    The aim of preoperative fasting is to prevent regurgitation and pulmonary aspiration while limiting potential problems of thirst, dehydration and hypoglycaemia. The American Society of Anaesthesiologists (ASA) has suggested guidelines for preoperative fasting for children undergoing elective surgery. We did a postal survey to determine the current practice regarding these guidelines amongst all specialist registrars in anaesthesia in Ireland. A questionnaire was sent to all specialist registrars in anaesthesia (90 in total), 60 (67%) were returned and analysed. The question asked was how long children should be kept fasting before elective surgery. The results of our survey suggest that most of the respondents are following the ASA guidelines for clear fluids and solids however there were differing opinion regarding the duration of fasting for formula milk and breast milk. In conclusion, we would recommend greater awareness and collaboration between anaesthetists, nurses and surgeons to ensure that fasting instructions are consistent with the ASA guidelines and that patient and their parents understand these directives as well.

  17. LiTrack A Fast longitudinal phase space tracking code with graphical user interface

    CERN Document Server

    Emma, Paul

    2005-01-01

    Many linear accelerators, such as linac-based light sources and linear colliders, apply longitudinal phase space manipulations in their design, including electron bunch compression and wakefield-induced energy spread control. Several computer codes handle such issues, but most require detailed information on the transverse focusing lattice. In fact, in most linear accelerators, the transverse distributions do not significantly affect the longitudinal, and can be ignored initially. This allows the use of a fast 2D code to study longitudinal aspects without time-consuming considerations of the transverse focusing. LiTrack is based on a 15-year old code (same name) originally written by one of us (KB), which is now a MATLAB-based code with additional features, such as a graphical user interface and output plotting. The single-bunch tracking includes RF acceleration, bunch compression to 3rd order, geometric and resistive wakefields, aperture limits, synchrotron radiation, and flexible output plotting. The code w...

  18. Synthesis and Application of a Full Water-Soluble and Red-Emitting Chemosensor Based on Phenoxazinium for Copper(ll) Ions%Synthesis and Application of a Full Water-Soluble and Red-Emitting Chemosensor Based on Phenoxazinium for Copper(ll) Ions

    Institute of Scientific and Technical Information of China (English)

    闫保龙; 孙如; 葛健锋; 许玉杰; 张欠欠; 杨学波; 路建美

    2012-01-01

    A phenoxazinium-based chemosensor (1) bearing di(2-picolyl)amine unit was successfully synthesized. The result shows that it is a red-emitting and full water-soluble chemosensor for the selective detection of Cu2+ in pure water. The fluorescence on-off mechanism was studied by ab initio calculations. To confirm the suitability of 1 for biological applications, it was employed in the fluorescence detection of the intracellular Cu2+ with cultured KB cells. The results indicated that 1 had good membrane permeability and could be useful for the fluorescence micro- scopic imaging.

  19. HI intensity mapping with FAST

    CERN Document Server

    Bigot-Sazy, Marie-Anne; Battye, Richard A; Browne, Ian W A; Chen, Tianyue; Dickinson, Clive; Harper, Stuart; Maffei, Bruno; Olivari, Lucas C; Wilkinson, Peter N

    2015-01-01

    We discuss the detectability of large-scale HI intensity fluctuations using the FAST telescope. We present forecasts for the accuracy of measuring the Baryonic Acoustic Oscillations and constraining the properties of dark energy. The FAST $19$-beam L-band receivers ($1.05$--$1.45$ GHz) can provide constraints on the matter power spectrum and dark energy equation of state parameters ($w_{0},w_{a}$) that are comparable to the BINGO and CHIME experiments. For one year of integration time we find that the optimal survey area is $6000\\,{\\rm deg}^2$. However, observing with larger frequency coverage at higher redshift ($0.95$--$1.35$ GHz) improves the projected errorbars on the HI power spectrum by more than $2~\\sigma$ confidence level. The combined constraints from FAST, CHIME, BINGO and Planck CMB observations can provide reliable, stringent constraints on the dark energy equation of state.

  20. Printtimalliston suunnitteluprosessi fast fashion -yritykselle

    OpenAIRE

    Heikkilä, Inna

    2016-01-01

    Opinnäytetyöni käsittelee suunnitteluprosessia fast fashion -yrityksessä. Oman prosessini kautta selvitän, millaisia vaikutuksia fast fashion -ilmiöllä on tekstiilisuunnittelijan työhön ja, mitä suunnittelijalta vaaditaan nopeatempoisessa suunnitteluprosessissa. Opinnäytetyön toimeksiantajana toimii Seppälä. Toiminnallinen osuus koostuu viiden t-paitaprintin suunnittelusta Seppälä Womanin elokuun 2016 mallistoon Seppälän suunnittelijoiden Katja Tuhkasaaren ja Elina Savolaisen ohjauksessa. Teo...

  1. A Fast Fractional Difference Algorithm

    DEFF Research Database (Denmark)

    Jensen, Andreas Noack; Nielsen, Morten Ørregaard

    We provide a fast algorithm for calculating the fractional difference of a time series. In standard implementations, the calculation speed (number of arithmetic operations) is of order T 2, where T is the length of the time series. Our algorithm allows calculation speed of order T logT . For mode......We provide a fast algorithm for calculating the fractional difference of a time series. In standard implementations, the calculation speed (number of arithmetic operations) is of order T 2, where T is the length of the time series. Our algorithm allows calculation speed of order T log...

  2. Fast Setting Cement - Literature Survey

    Science.gov (United States)

    1973-01-01

    materials does produce early set times and high strengths . It is re- ported, also, that the addition of 1.5 percent Ca"l 2 to clinkers contain- Ing more than...produce high strength concrete. They include addition of iron asggregate, physical treatment of clinker , ’ addition of highly reactive SiO2 or Ca0, and...Fast-Fix. The Western Co. has developed materials designated as Fast-Fix with rapid setting and high strength properties. Published data show i .1

  3. Fast Food Problems and Responsibilities

    Institute of Scientific and Technical Information of China (English)

    Zhang; Jing

    2015-01-01

    <正>People are living in a world that acquires almost everyone to chase efficiency and speed.The real inner quality of daily life is not approximately taken into people’s consideration.Fast food is one of the common model reactions of this rapid developing society.Recently,it’s getting popular that people would rather have fast food to save time for other extra work.Problems and public concern are following that kind of life condition and someone or something should be put blame on for this.

  4. Genetic mapping of the LOBED LEAF 1 (ClLL1) gene to a 127.6-kb region in watermelon (Citrullus lanatus L.).

    Science.gov (United States)

    Wei, Chunhua; Chen, Xiner; Wang, Zhongyuan; Liu, Qiyan; Li, Hao; Zhang, Yong; Ma, Jianxiang; Yang, Jianqiang; Zhang, Xian

    2017-01-01

    The lobed leaf character is a unique morphologic trait in crops, featuring many potential advantages for agricultural productivity. Although the majority of watermelon varieties feature lobed leaves, the genetic factors responsible for lobed leaf formation remain elusive. The F2:3 leaf shape segregating population offers the opportunity to study the underlying mechanism of lobed leaf formation in watermelon. Genetic analysis revealed that a single dominant allele (designated ClLL1) controlled the lobed leaf trait. A large-sized F3:4 population derived from F2:3 individuals was used to map ClLL1. A total of 5,966 reliable SNPs and indels were identified genome-wide via a combination of BSA and RNA-seq. Using the validated SNP and indel markers, the location of ClLL1 was narrowed down to a 127.6-kb region between markers W08314 and W07061, containing 23 putative ORFs. Expression analysis via qRT-PCR revealed differential expression patterns (fold-changes above 2-fold or below 0.5-fold) of three ORFs (ORF3, ORF11, and ORF18) between lobed and non-lobed leaf plants. Based on gene annotation and expression analysis, ORF18 (encoding an uncharacterized protein) and ORF22 (encoding a homeobox-leucine zipper-like protein) were considered as most likely candidate genes. Furthermore, sequence analysis revealed no polymorphisms in cDNA sequences of ORF18; however, two notable deletions were identified in ORF22. This study is the first report to map a leaf shape gene in watermelon and will facilitate cloning and functional characterization of ClLL1 in future studies.

  5. The human NTT gene: Identification of a novel 17-kb noncoding nuclear RNA expressed in activated CD4{sup +} T cells

    Energy Technology Data Exchange (ETDEWEB)

    Liu, A.Y.; Torchia, B.S.; Migeon, B.R. [Johns Hopkins Univ. School of Medicine, Baltimore, MD (United States)] [and others

    1997-01-15

    We describe the cloning and characterization of the NTT gene (noncoding transcript in T cells), identified by differential display RT-PCR based on the differential presence of its transcript in a subset of activated, human CD4{sup +} T-cell clones. The full-length cDNA and genomic sequences were cloned and found to produce a 17-kb transcript that is polyadenylated, but is not spliced. Consistent with the transcript`s nuclear predominance, NTT has no open reading frame larger than 270 bp. It is transcribed in a select subset of CD4{sup +} T-cell clones propagated in vitro. Its transcription can also be induced in freshly isolated T cells by in vitro activation with PHA or with PMA and ionomycin. In vivo, NTT transcripts are found only in activated, but not resting, T cells. Transcripts were absent in a variety of lymphoid cell lines and transformed lines from other tissues. NTT is a new member of the small group of genes including XIST (X{sub i}-specific transcript), H19, and IPW (imprinted gene in the Prader-Willi syndrome region), which are transcribed but not translated, and may have a role in the regulation of neighboring genes. XIST, H19, and IPW exhibit monoallelic expression, but both NTT alleles are expressed in CD4{sup +} T-cell clones. Southern blot and fluorescence in situ hybridization analyses show that NTT is a single-copy gene residing in chromosome 6q23-q24, near the interferon-{gamma} receptor gene (IFN-{gamma}R). Their proximity and shared expression pattern suggest a possible functional relationship. 57 refs., 10 figs., 1 tab.

  6. Purification, gene cloning, and biochemical characterization of a β-glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. KB0549.

    Directory of Open Access Journals (Sweden)

    Arun Nair

    Full Text Available The triglucoside of sesaminol, i.e., 2,6-O-di(β-D-glucopyranosyl-β-D- glucopyranosylsesaminol (STG, occurs abundantly in sesame seeds and sesame oil cake and serves as an inexpensive source for the industrial production of sesaminol, an anti-oxidant that displays a number of bioactivities beneficial to human health. However, STG has been shown to be highly resistant to the action of β-glucosidases, in part due to its branched-chain glycon structure, and these circumstances hampered the efficient utilization of STG. We found that a strain (KB0549 of the genus Paenibacillus produced a novel enzyme capable of efficiently hydrolyzing STG. This enzyme, termed PSTG, was a tetrameric protein consisting of identical subunits with an approximate molecular mass of 80 kDa. The PSTG gene was cloned on the basis of the partial amino acid sequences of the purified enzyme. Sequence comparison showed that the enzyme belonged to the glycoside hydrolase family 3, with significant similarities to the Paenibacillus glucocerebrosidase (63% identity and to Bgl3B of Thermotoga neapolitana (37% identity. The recombinant enzyme (rPSTG was highly specific for β-glucosidic linkage, and k cat and k cat/K m values for the rPSTG-catalyzed hydrolysis of p-nitrophenyl-β-glucopyraniside at 37°C and pH 6.5 were 44 s(-1 and 426 s(-1 mM(-1, respectively. The specificity analyses also revealed that the enzyme acted more efficiently on sophorose than on cellobiose and gentiobiose. Thus, rPSTG is the first example of a β-glucosidase with higher reactivity for β-1,2-glucosidic linkage than for β-1,4- and β-1,6-glucosidic linkages, as far as could be ascertained. This unique specificity is, at least in part, responsible for the enzyme's ability to efficiently decompose STG.

  7. Gene Flow Across Genus Barriers - Conjugation of Dinoroseobacter shibae's 191-kb Killer Plasmid into Phaeobacter inhibens and AHL-mediated Expression of Type IV Secretion Systems.

    Science.gov (United States)

    Patzelt, Diana; Michael, Victoria; Päuker, Orsola; Ebert, Matthias; Tielen, Petra; Jahn, Dieter; Tomasch, Jürgen; Petersen, Jörn; Wagner-Döbler, Irene

    2016-01-01

    Rhodobacteraceae harbor a conspicuous wealth of extrachromosomal replicons (ECRs) and therefore the exchange of genetic material via horizontal transfer has been supposed to be a major evolutionary driving force. Many plasmids in this group encode type IV secretion systems (T4SS) that are expected to mediate transfer of proteins and/or DNA into host cells, but no experimental evidence of either has yet been provided. Dinoroseobacter shibae, a species of the Roseobacter group within the Rhodobacteraceae family, contains five ECRs that are crucial for anaerobic growth, survival under starvation and the pathogenicity of this model organism. Here we tagged two syntenous but compatible RepABC-type plasmids of 191 and 126-kb size, each encoding a T4SS, with antibiotic resistance genes and demonstrated their conjugational transfer into a distantly related Roseobacter species, namely Phaeobacter inhibens. Pulsed field gel electrophoresis showed transfer of those replicons into the recipient both individually but also together documenting the efficiency of conjugation. We then studied the influence of externally added quorum sensing (QS) signals on the expression of the T4SS located on the sister plasmids. A QS deficient D. shibae null mutant (ΔluxI1 ) lacking synthesis of N-acyl-homoserine lactones (AHLs) was cultivated with a wide spectrum of chemically diverse long-chain AHLs. All AHLs with lengths of the acid side-chain ≥14 reverted the ΔluxI1 phenotype to wild-type. Expression of the T4SS was induced up to log2 ∼3fold above wild-type level. We hypothesize that conjugation in roseobacters is QS-controlled and that the QS system may detect a wide array of long-chain AHLs at the cell surface.

  8. AISLAMIENTO DE CLONES CON ACTIVIDAD ENDO-b-1,4-GLUCANASA A PARTIR DE UN SEGMENTO DE ADN DE 13KB DE Clostridium sp IBUN22A

    Directory of Open Access Journals (Sweden)

    I. Roncancio-Sánchez

    2006-06-01

    Full Text Available La producción de combustibles, solventes y algunos productos químicos a partir de sustratos celulósicos usando microorganismos ofrece una ventaja frente a los de origen fósil. Un acercamiento prometedor ha sido la implementación de ingeniería genética utilizando genes de enzimas involucradas en la degradación de desechos celulósicos. En la última década se han generado bibliotecas genéticas para proveer enzimas celulolíticas, que hagan el proceso más rentable, lo cual permitiría aprovechar mejor residuos celulósicos disponibles. Este trabajo describe el aislamiento de dos fragmentos de ADN que expresan actividad endo-β-1,4-glucanasa, a partir de un segmento ADN de 13Kb (clon 02080-25 aislado de una biblioteca genómica de la cepa nativa Clostridium sp IBUN22A. El aislamiento de la región codificadora se realizó a través de pruebas de inducción de la actividad, análisis por restricción del segmento y de una sub-biblioteca con la enzima Sau3A I. 325 clones fueron obtenidos, de los cuales 271 tenían inserto. El tamizaje molecular de estos últimos mostró que siete clones presentaron tamaños entre 3500pb y 7000pb y el tamizaje enzimático con carboximetilcelulosa como sustrato permitió el aislamiento de los clones pBSh-37 y pBSh-26 con la actividad endo-β-1,4-glucanasa original, de tamaños de inserto de 627pb y 879pb respectivamente. Este trabajo es el punto de partida para el aislamiento de enzimas de alto potencial biotecnológico.

  9. Greater than X kb: a quantitative assessment of preservation conditions on genomic DNA quality, and a proposed standard for genome-quality DNA.

    Science.gov (United States)

    Mulcahy, Daniel G; Macdonald, Kenneth S; Brady, Seán G; Meyer, Christopher; Barker, Katharine B; Coddington, Jonathan

    2016-01-01

    Advances in biodiversity genomic sequencing will increasingly depend on the availability of DNA samples-and their quantifiable metadata-preserved in large institutional biorepositories that are discoverable to the scientific community. Improvements in sequencing technology constantly provide longer reads, such that longer fragment length, higher molecular weight, and overall "genome-quality" DNA (gDNA) will be desirable. Ideally, biorepositories should publish numerical scale measurements of DNA quality useful to the user community. However, the most widely used technique to evaluate DNA quality, the classic agarose gel, has yet to be quantified. Here we propose a simple and economical method using open source image analysis software to make gDNA gel images quantifiable, and propose percentage of gDNA "greater than X kb" as a standard of comparison, where X is a band from any widely used DNA ladder with desirably large band sizes. We employ two metadata standards ("DNA Threshold" and "Percent above Threshold") introduced as part of the Global Genome Biodiversity Network (GGBN) Darwin Core extension. We illustrate the method using the traditionally used HindIII ladder and the 9,416 base-pair (bp) band as a standard. We also present data, for two taxa, a vertebrate (fish) and an invertebrate (crab), on how gDNA quality varies with seven tissue preservation methods, time since death, preservation method (i.e. buffers vs. cold temperatures), and storage temperature of various buffers over time. Our results suggest that putting tissue into a buffer prior to freezing may be better than directly into ultra-cold conditions.

  10. Analysis of a novel 8.9kb cryptic plasmid from Bacteroides uniformis, its long-term stability and spread within human microbiota.

    Science.gov (United States)

    Shkoporov, Andrei N; Khokhlova, Ekaterina V; Kulagina, Elena V; Smeianov, Vladimir V; Kuchmiy, Anna A; Kafarskaya, Lyudmila I; Efimov, Boris A

    2013-03-01

    The analysis of plasmid content in dominant Bacteroidales order intestinal strains isolated from the same child at a 5 year interval identified a 8.9 kb plasmid in Bacteroides uniformis BUN24 strain isolated at age 6 and indistinguishably sized plasmids in the isolates of B. uniformis, B. vulgatus, B. intesinalis, and Parabacteroides distasonis at age 11. We sequenced a B. uniformis BUN24 plasmid, designated pBUN24, and using molecular surveys of diverse species we established that this 8944bp molecule (G+C content 43.5%) represents a novel family of small cryptic Bacteroidales plasmids. The replication region of pBUN24 was experimentally localized to a 1707-bp fragment that includes a putative repA gene, coding for a protein of Rep_3 superfamily of replication proteins of theta-type plasmids preceded by a putative iteron-containing origin of replication. The other open reading frames (ORFs) identified in pBUN24 sequence include a putative tad-ata-type toxin-antitoxin and mobA-mobB mobilization modules, as well as seven additional cryptic ORFs. The interaction of Tad and Ada components demonstrated by a pull-down assay and the toxicity of Tad in Escherichia coli host suggests the functionality of the plasmid addiction module. Re-sequencing of plasmids in two Bacteroides strains isolated at the age of 11 showed 100% nucleotide identity to pBUN24. This data supports the notion that this plasmid is transmissible to other Bacteroidales strains in the natural ecosystem. The possible roles of toxin-antitoxin system and other proteins encoded by pBUN24 in providing an apparent ecological advantage to the plasmid-harbouring strains of a bacterial symbiont in the human gut deserve further investigation. Copyright © 2012 Elsevier Inc. All rights reserved.

  11. Dwarfism and Altered Craniofacial Development in Rabbits Is Caused by a 12.1 kb Deletion at the HMGA2 Locus.

    Science.gov (United States)

    Carneiro, Miguel; Hu, Dou; Archer, John; Feng, Chungang; Afonso, Sandra; Chen, Congying; Blanco-Aguiar, José A; Garreau, Hervé; Boucher, Samuel; Ferreira, Paula G; Ferrand, Nuno; Rubin, Carl-Johan; Andersson, Leif

    2017-02-01

    The dwarf phenotype characterizes the smallest of rabbit breeds and is governed largely by the effects of a single dwarfing allele with an incompletely dominant effect on growth. Dwarf rabbits typically weigh under 1 kg and have altered craniofacial morphology. The dwarf allele is recessive lethal and dwarf homozygotes die within a few days of birth. The dwarf phenotype is expressed in heterozygous individuals and rabbits from dwarf breeds homozygous for the wild-type allele are normal, although smaller when compared to other breeds. Here, we show that the dwarf allele constitutes a ∼12.1 kb deletion overlapping the promoter region and first three exons of the HMGA2 gene leading to inactivation of this gene. HMGA2 has been frequently associated with variation in body size across species. Homozygotes for null alleles are viable in mice but not in rabbits and probably not in humans. RNA-sequencing analysis of rabbit embryos showed that very few genes (4-29 genes) were differentially expressed among the three HMGA2/dwarf genotypes, suggesting that dwarfism and inviability in rabbits are caused by modest changes in gene expression. Our results show that HMGA2 is critical for normal expression of IGF2BP2, which encodes an RNA-binding protein. Finally, we report a catalog of regions of elevated genetic differentiation between dwarf and normal-size rabbits, including LCORL-NCAPG, STC2, HOXD cluster, and IGF2BP2 Levels and patterns of genetic diversity at the LCORL-NCAPG locus further suggest that small size in dwarf breeds was enhanced by crosses with wild rabbits. Overall, our results imply that small size in dwarf rabbits results from a large effect, loss-of-function (LOF) mutation in HMGA2 combined with polygenic selection. Copyright © 2017 by the Genetics Society of America.

  12. Cytotoxic evaluation of medicinal smoke \\"Anbar Nasara\\" against cancer cells (Hela & KB and a normal cell line (L929, using MTT assay

    Directory of Open Access Journals (Sweden)

    hojat Sadeghi-aliabadi

    2013-09-01

    Full Text Available Background: Medicinal smoke has been in natural health products by many nations all over the worlds. Dung's smoke called "Anbar Nasara" is one of the most used medicinal smokes in Iranian traditional medicine. Anber Nasara has been administered as inhalation in the treatment of viral or bacterial infections as well as for tumors and cysts in vaginal diseases in women. Its cytotoxic effects have been proposed in these studies. Materials and Methods: Dung's of female donkey have been collected from Shahreza, Isfahan province in summer 2011. The samples were burned in a specially designed apparatus and its smoke was collected in n-hexane solvent over ice-cold water. Solvent was evaporated in the air and dried residue was dissolved in DMSO and diluted in RPMI So that the final concentrations of 0.4, 0.3, 0.25, 0.2, 0.125, 0.1, 0.0625, 0.0312 mg/ml were obtained for MTT assay as a cytotoxic evaluation method against two cancer cells (KB and Hela and a normal one (L929. After 48h incubation percent cell survival and IC50 values were obtained using ELISA plate reader at 540nm. Results: Results showed that dung's smoke were cytotoxic in tested concentrations against cancer cell lines in a dose dependent manner. The IC50 was calculated ≥ 0.2 mg/ml. Conclusion: Dung's smoke consists of too many constituents and its cytotoxic effect may belong to one or complexes of constituents. More researches are under way to clarify these aspects of the study.

  13. Broader utilization of origins of DNA replication in cancer cell lines along a 78 kb region of human chromosome 2q34.

    Science.gov (United States)

    Valenzuela, Manuel S; Hu, Lan; Lueders, John; Walker, Robert; Meltzer, Paul S

    2012-01-01

    Human DNA replication depends on the activation of thousands of origins distributed within the genome. The actual distribution of origins is not known, nor whether this distribution is unique to a cell type, or if it changes with the proliferative state of the cell. In this study, we have employed a real-time PCR-based nascent strand DNA abundance assay, to determine the location of origins along a 78 kb region on Chr2q34. Preliminary studies using nascent DNA strands isolated from either HeLa and normal skin fibroblast cells showed that in both cell lines peaks of high origin activity mapped in similar locations. However, the overall origin profile in HeLa cells corresponded to broad origin activation zones, whereas in fibroblasts a more punctuated profile of origin activation was observed. To investigate the relevance of this differential origin profile, we compared the origin distribution profiles in breast cancer cell lines MDA-MB-231, BT-474, and MCF-7, to their normal counterpart MCF-10A. In addition, the CRL7250 cell line was also used as a normal control. Our results validated our earlier observation and showed that the origin profile in normal cell lines exhibited a punctuated pattern, in contrast to broader zone profiles observed in the cancer cell lines. A quantitative analysis of origin peaks revealed that the number of activated origins in cancer cells is statistically larger than that obtained in normal cells, suggesting that the flexibility of origin usage is significantly increased in cancer cells compared to their normal counterparts.

  14. [Variations of soil fertility level in red soil region under long-term fertilization].

    Science.gov (United States)

    Yu, Han-qing; Xu, Ming-gang; Lü, Jia-long; Bao, Yao-xian; Sun, Nan; Gao, Ju-sheng

    2010-07-01

    Based on the long-term (1982-2007) field experiment of "anthropogenic mellowing of raw soil" at the Qiyang red soil experimental station under Chinese Academy of Agricultural Sciences, and by using numerical theory, this paper studied the variations of the fertility level of granite red soil, quaternary red soil, and purple sandy shale soil under six fertilization patterns. The fertilization patterns included non-fertilization (CK), straw-returning without fertilizers (CKR), chemical fertilization (NPK), NPK plus straw-return (NPKR), rice straw application (M), and M plus straw-return (MR). The soil integrated fertility index (IFI) was significantly positively correlated with relative crop yield, and could better indicate soil fertility level. The IFI values of the three soils all were in the order of NPK, NPKR > M, MR > CK, CKR, with the highest value in treatment NPKR (0.77, 0.71, and 0.71 for granite red soil, quaternary red soil, and purple sandy shale soil, respectively). Comparing with that in the treatments of no straw-return, the IFI value in the treatments of straw return was increased by 6.72%-18.83%. A turning point of the IFI for all the three soils was observed at about 7 years of anthropogenic mellowing, and the annual increasing rate of the IFI was in the sequence of purple sandy shale soil (0.016 a(-1)) > quaternary red clay soil (0.011 a(-1)) > granite red soil (0.006 a(-1)). It was suggested that a combined application of organic and chemical fertilizers and/or straw return could be an effective and fast measure to enhance the soil fertility level in red soil region.

  15. Evaluation of changes in blood hematological and biochemical parameters in response to Islamic fasting and regular physical activity in male and female subjects

    Directory of Open Access Journals (Sweden)

    Seyyed Reza Attarzadeh Hosseini

    2015-09-01

    Full Text Available Introduction: Fasting during Ramadan is a religious obligation for healthy adult Muslims. This study aimed to investigate the effects of Ramadan fasting and physical activity on blood hematological and biochemical parameters in male and female subjects. Methods: In this study, 50 healthy subjects were randomly assigned to four groups: male control fasting (MCF group (n=13, male training fasting (MTF group (n=13, female control fasting (FCF group (n=12, and female training fasting (FTF group (n=12, and were compared in two stages (before and after the intervention. For inter- and intra-group comparisons, a repeated measure ANOVA was applied. Results: The results showed a significant decline in body weight, body mass index, body fat percentage, and waist-to-hip ratio after Ramadan fasting, compared to the pre-Ramadan period. Also, significant changes were observed in red blood cell count and hematocrit level (P

  16. Allergic reactions in red tattoos

    DEFF Research Database (Denmark)

    Hutton Carlsen, K; Køcks, M; Sepehri, M

    2016-01-01

    AIM: The aim of this study was to assess the feasibility of Raman spectroscopy as a screening technique for chemical characterisation of tattoo pigments in pathologic reacting tattoos and tattoo ink stock products to depict unsafe pigments and metabolites of pigments. MATERIALS/METHODS: Twelve...... to be feasible for chemical analysis of red pigments in allergic reactions. Raman spectroscopy has a major potential for fingerprint screening of problematic tattoo pigments in situ in skin, ex vivo in skin biopsies and in tattoo ink stock products, thus, to eliminate unsafe ink products from markets....

  17. Control of poultry red mites

    DEFF Research Database (Denmark)

    Kilpinen, Ole; Steenberg, Tove

    2008-01-01

    The poultry red mite (PRM), Dermanyssus gallinae, is the most important ectoparasite in European egg production. The mites hide in cracks and crevices in the near vicinity of the resting places of the birds, coming out to feed mainly during the night. Under favourable conditions the population can...... grow rapidly, leading to serious problems. Large mite populations may cause anaemia or even death to the poultry, but also in lower numbers mites may be a nuisance to the birds causing decreased egg production and egg quality. Furthermore, they may have the potential of acting as reservoir...

  18. Solar Drying of Red Mud

    Science.gov (United States)

    Chandler, John L.

    Solar drying of thickened red mud is the latest method for its disposal in an economical and environmentally acceptable way. Two years full scale experience with this method in Jamaica has shown that its success depends on accurate grading of the solar drying area and accurate control of the pre-dewatering of the mud in the alumina plant. Experience with the use of deep thickening for pre-dewatering is described, together with a novel method for measurement and control of thickened mud rheology.

  19. N-Pyridineium-2-yl Darrow Red analogue: unique near-infrared lysosome-biomarker for the detection of cancer cells.

    Science.gov (United States)

    He, Dan-Dan; Liu, Wu; Sun, Ru; Fan, Chen; Xu, Yu-Jie; Ge, Jian-Feng

    2015-02-03

    The lysosome-targetable OFF-ON type pH sensor that does not emit at pH = 4.0 is adopted for the selective detection of cancer cells, and the acidity difference of lysosomes in cancer and normal cells is verified. Three pH probes based on Darrow Red derivatives were designed and prepared that were demonstrated to be lysosome-specific biomarkers with inducible emission at 580-850 nm by the comparable in cellular imaging assays using HeLa, KB, and V79 cells. Of these, a pyridineium-2-yl Darrow Red analogue with a pKa of 2.4 was found to be a lysosome tracker for cancer cells, it is a unique pH sensor for the optical identification and distinction of cancer cells from normal cells and has potential application as a fluorescent biomaker of cancer cells in in vitro assays.

  20. A putative helicase, the SUA5, PMR1, tRNALys1 genes and four open reading frames have been detected in the DNA sequence of an 8.8 kb fragment of the left arm of chromosome VII of Saccharomyces cerevisiae.

    Science.gov (United States)

    Klima, R; Coglievina, M; Zaccaria, P; Bertani, I; Bruschi, C V

    1996-09-01

    We report the sequence of an 8.8 kb segment of DNA from the left arm of chromosome VII of Saccharomyces cerevisiae. The sequence reveals seven open reading frames (ORFs) G1651, G1654, G1660, G1663, G1666, G1667 and G1669 greater than 100 amino acids in length and the tRNALys1 gene. ORF G1651 shows 100% identity with the ROK1 protein which is a putative RNA helicase of the 'DEAD box' protein family. ORF G1654 exhibits a motif highly conserved in ATP/GTP binding proteins generally referred to as 'P-loop'. From FastA analysis, G1660 and G1666 were found to be previously sequenced genes, respectively SUA5 and PMR1. The three other ORFs identified are partially (G1663) or completely (G1667 and G1669) overlapping with the PMR1 sequence on the complementary strand. This feature, together with their low codon adaptation indexes and the absence of significant homology with known proteins suggest that they do not correspond to real genes.

  1. Effect of fast and slow pranayama practice on cognitive functions in healthy volunteers.

    Science.gov (United States)

    Sharma, Vivek Kumar; M, Rajajeyakumar; S, Velkumary; Subramanian, Senthil Kumar; Bhavanani, Ananda B; Madanmohan; Sahai, Ajit; Thangavel, Dinesh

    2014-01-01

    To compare the cumulative effect of commonly practised slow and fast pranayama on cognitive functions in healthy volunteers. 84 participants who were in self-reported good health, who were in the age group of 18-25 years, who were randomized to fast pranayama, slow pranayama and control group with 28 participants in each group. Fast pranayama included kapalabhati, bhastrika and kukkuriya. Slow pranayama included nadishodhana, Pranav and Savitri. Respective pranayama training was given for 35 minutes, three times per week, for a duration of 12 weeks under the supervision of a certified yoga trainer. Parameters were recorded before and after 12 weeks of intervention: Perceived stress scale (PSS), BMI, waist to hip ratio and cognitive parameters-letter cancellation test, trail making tests A and B, forward and reverse digit spans and auditory and visual reaction times for red light and green light. Inter-group comparison was done by one way ANOVA and intra-group comparison was done by paired t-test. Executive functions, PSS and reaction time improved significantly in both fast and slow pranayama groups, except reverse digit span, which showed an improvement only in fast pranayama group. In addition, percentage reduction in reaction time was significantly more in the fast pranayama group as compared to that in slow pranayama group. Both types of pranayamas are beneficial for cognitive functions, but fast pranayama has additional effects on executive function of manipulation in auditory working memory, central neural processing and sensory-motor performance.

  2. Red is romantic, but only for feminine females: sexual dimorphism moderates red effect on sexual attraction.

    Science.gov (United States)

    Wen, Fangfang; Zuo, Bin; Wu, Yang; Sun, Shan; Liu, Ke

    2014-08-08

    Previous researchers have documented that the color red enhances one's sexual attraction to the opposite sex. The current study further examined the moderating role of sexual dimorphism in red effects. The results indicated that red enhanced men's sexual attraction to women with more feminine facial characteristics but had no effect on ratings of perceived general attractiveness. Red clothing also had a marginally significant effect on men's sexual attractiveness. In addition, regardless of sexual dimorphism cues, male participants rated women with red as warmer and more competent. The underlying mechanisms of the red effect, the limitations of the current study, and suggestions for future directions are discussed.

  3. Fast data processing with Spark

    CERN Document Server

    Sankar, Krishna

    2015-01-01

    Fast Data Processing with Spark - Second Edition is for software developers who want to learn how to write distributed programs with Spark. It will help developers who have had problems that were too big to be dealt with on a single computer. No previous experience with distributed programming is necessary. This book assumes knowledge of either Java, Scala, or Python.

  4. Fast electromagnetic field strength probes

    NARCIS (Netherlands)

    Leferink, Frank; Serra, Ramiro

    2013-01-01

    Diode detectors and thermocouple detectors are conventionally used to measure electromagnetic field strength. Both detectors have some disadvantages for applications where a fast response and a high dynamic range is required. The diode detector is limited in dynamic range. The dynamic range is impor

  5. Enhanced Model for Fast Ignition

    Energy Technology Data Exchange (ETDEWEB)

    Mason, Rodney J. [Research Applications Corporation, Los Alamos, NM (United States)

    2010-10-12

    Laser Fusion is a prime candidate for alternate energy production, capable of serving a major portion of the nation's energy needs, once fusion fuel can be readily ignited. Fast Ignition may well speed achievement of this goal, by reducing net demands on laser pulse energy and timing precision. However, Fast Ignition has presented a major challenge to modeling. This project has enhanced the computer code ePLAS for the simulation of the many specialized phenomena, which arise with Fast Ignition. The improved code has helped researchers to understand better the consequences of laser absorption, energy transport, and laser target hydrodynamics. ePLAS uses efficient implicit methods to acquire solutions for the electromagnetic fields that govern the accelerations of electrons and ions in targets. In many cases, the code implements fluid modeling for these components. These combined features, "implicitness and fluid modeling," can greatly facilitate calculations, permitting the rapid scoping and evaluation of experiments. ePLAS can be used on PCs, Macs and Linux machines, providing researchers and students with rapid results. This project has improved the treatment of electromagnetics, hydrodynamics, and atomic physics in the code. It has simplified output graphics, and provided new input that avoids the need for source code access by users. The improved code can now aid university, business and national laboratory users in pursuit of an early path to success with Fast Ignition.

  6. Micro mixer with fast diffusion

    NARCIS (Netherlands)

    Miyake, Ryo; Miyake, R.; Lammerink, Theodorus S.J.; Elwenspoek, Michael Curt; Fluitman, J.H.J.

    1993-01-01

    A new concept for micro-mixing of liquid is introduced and feasibility is demonstrated. The mixer allows fast mixing of small amounts of two liquids and it is application to micro-liquid handling systems [1]. The mixer has a channel for the liquid, an inlet port for the reagent, and a 2.2 mm × 2 mm

  7. Fast, Massively Parallel Data Processors

    Science.gov (United States)

    Heaton, Robert A.; Blevins, Donald W.; Davis, ED

    1994-01-01

    Proposed fast, massively parallel data processor contains 8x16 array of processing elements with efficient interconnection scheme and options for flexible local control. Processing elements communicate with each other on "X" interconnection grid with external memory via high-capacity input/output bus. This approach to conditional operation nearly doubles speed of various arithmetic operations.

  8. Fast Timing for Collider Detectors

    CERN Document Server

    CERN. Geneva

    2017-01-01

    Advancements in fast timing particle detectors have opened up new possibilities to design collider detectors that fully reconstruct and separate event vertices and individual particles in the time domain. The applications of these techniques are considered for the physics at HL-LHC.

  9. A re-sequencing based assessment of genomic heterogeneity and fast neutron-induced deletions in a common bean cultivar

    Directory of Open Access Journals (Sweden)

    Jamie A. O'Rourke

    2013-06-01

    Full Text Available A small fast neutron mutant population has been established from Phaseolus vulgaris cv. Red Hawk. We leveraged the available P. vulgaris genome sequence and high throughput next generation DNA sequencing to examine the genomic structure of five Phaseolus vulgaris cv. Red Hawk fast neutron mutants with striking visual phenotypes. Analysis of these genomes identified three classes of structural variation; between cultivar variation, natural variation within the fast neutron mutant population, and fast neutron induced mutagenesis. Our analyses focused on the latter two classes. We identified 23 large deletions (>40 bp common to multiple individuals, illustrating residual heterogeneity and regions of structural variation within the common bean cv. Red Hawk. An additional 18 large deletions were identified in individual mutant plants. These deletions, ranging in size from 40 bp to 43,000 bp, are potentially the result of fast neutron mutagenesis. Six of the 18 deletions lie near or within gene coding regions, identifying potential candidate genes causing the mutant phenotype.

  10. The multilevel fast multipole algorithm (MLFMA) for solving large-scale computational electromagnetics problems

    CERN Document Server

    Ergul, Ozgur

    2014-01-01

    The Multilevel Fast Multipole Algorithm (MLFMA) for Solving Large-Scale Computational Electromagnetic Problems provides a detailed and instructional overview of implementing MLFMA. The book: Presents a comprehensive treatment of the MLFMA algorithm, including basic linear algebra concepts, recent developments on the parallel computation, and a number of application examplesCovers solutions of electromagnetic problems involving dielectric objects and perfectly-conducting objectsDiscusses applications including scattering from airborne targets, scattering from red

  11. Woman Village Leader and The Red Peppers

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    YU Hua, 28, deputy head of Sanduan Village is very proud of her village. As she welcomes visitors, she is sure to let them know: "Our red peppers were reported in the Chinese Population News." Red peppers grown in Sanduan Village are distinctive for their good quality, bright color, and pure hot taste Traders came to the village to buy red peppers. Thus their reputation grew quickly and the village head was even invited to Beijing to take part in a

  12. Transcriptional regulation of the gene for glucose transporter GLUT4 in skeletal muscle. Effects of diabetes and fasting.

    Science.gov (United States)

    Neufer, P D; Carey, J O; Dohm, G L

    1993-07-05

    GLUT4 glucose transporter protein and mRNA levels in rat skeletal muscle are decreased with streptozotocin (STZ)-induced diabetes and increased by fasting, indicating that GLUT4 expression may be regulated at the pretranslational level. The purpose of the present study was to determine whether GLUT4 is subject to transcriptional regulation in skeletal muscle under the altered metabolic conditions of diabetes and fasting. Nuclei were isolated from red and white portions of the quadriceps and gastrocnemius/plantaris muscles of control, 7-day STZ-diabetic, and 3-day fasted rats. STZ-induced diabetes resulted in a 35% reduction in GLUT4 transcription in red skeletal muscle and thus accounted for a major portion of the corresponding 50% reduction in GLUT4 mRNA observed in red skeletal muscle. STZ-induced diabetes had no significant effect on GLUT4 transcription or mRNA in white skeletal muscle. Fasting, however, significantly increased both GLUT4 transcription (2.2-fold) and mRNA (2.9-fold) in white skeletal muscle with no change detected for either parameter in red skeletal muscle. The nearly 2-fold higher steady-state GLUT4 mRNA in red versus white skeletal muscle of control rats was not associated with any difference in basal transcription. These findings demonstrate that expression of the GLUT4 glucose transporter protein in skeletal muscle is subject to regulation in vivo at the level of transcription of the GLUT4 gene. In addition, GLUT4 transcription is regulated in a fiber type-specific manner in response to the metabolic challenges elicited by STZ-induced diabetes and fasting.

  13. Antiproliferative and antibacterial activity evaluation of red ...

    African Journals Online (AJOL)

    sunny

    2014-10-22

    Oct 22, 2014 ... Key words: Rhodosorus marinus, red microalgae, antibacterial activity, antiproliferative activity, methanolic extract. ... subsequent production of metabolites (secondary ..... antioxidant molecules, such as vitamins, enzymes,.

  14. Red Sesbania Distribution - lines [ds81

    Data.gov (United States)

    California Department of Resources — This layer conatains line data for the red sesbania (Sesbania punicea) database. The database represents historic and current observations by various individuals of...

  15. First successful automated red cell exchange (erythrocytapheresis ...

    African Journals Online (AJOL)

    First successful automated red cell exchange (erythrocytapheresis) in ... PROMOTING ACCESS TO AFRICAN RESEARCH ... Hematopoietic Stem cell Transplantation (HSCT) remains the only curative therapy for Sickle Cell Disease (SCD).

  16. Red Sesbania Distribution - points [ds80

    Data.gov (United States)

    California Department of Resources — This layer contains point data for the red sesbania (Sesbania punicea) database. The database represents historic and current observations by various individuals of...

  17. Pure red cell aplasia and associated thymoma

    Directory of Open Access Journals (Sweden)

    Cristian Rosu

    2011-04-01

    Full Text Available Pure red cell aplasia is a rare cause of anemia, caused by an absence of red blood cell precursors in the bone marrow. It is usually a paraneoplastic syndrome, associated most commonly with large-cell granular lymphocyte leukemia but also thymoma. For patients who present both pure red cell aplasia and thymoma, thymectomy leads to an initial remission of the aplasia in 30% of cases. However, sustained remission may require the addition of medications such as corticosteroids, cyclospo­rine, or cyclophosphamide. We present a case of pure red cell aplasia associated with a thymoma in an otherwise healthy 80 year-old woman.

  18. Capillarization, mitochondrial densities, oxygen diffusion distances and innervation of red and white muscle of the lizard Dipsosaurus dorsalis.

    Science.gov (United States)

    Gleeson, T T; Nicol, C J; Johnston, I A

    1984-01-01

    The white and red regions of the iliofibularis muscle of the lizard Dipsosaurus dorsalis were analyzed using histologic and morphometric analysis. These regions are composed of fast glycolytic (FG) and both fast oxidative, glycolytic (FOG) and tonic fibers, respectively. Endplate morphology and number of endplates per fiber were estimated from fibers from both areas. Capillary volume densities of the red and white regions were quantified from transverse sections. Mitochondrial volume of fibers from the red and white regions were estimated from electron micrographs. All fibers from the white region of the iliofibularis possessed a single, well defined endplate, as did most red region fibers. The remaining red fibers (28 +/- 5%) possessed an average of 14.7 +/- 3 endplates each, distributed along the entire length of the fiber at intervals of approximately 1124 micron. Red fibers possessed twice the mitochondrial volume of white fibers (7.6 +/- 0.4%, red; 3.8 +/- 0.3%, white). Mitochondria were distributed uniformly through the fibers from both regions. Capillary anisotropy was low (gamma = 1.018) in both regions. Capillary densities of the red region (629 +/- 35 mm-2) were much greater than those of the corresponding white region (73 +/- 8 mm-2). The data indicate that capillary densities, mitochondrial volumes and theoretical diffusion distances correlate well with the oxidative capacity of lizard muscle fibers. Tonic fibers of this species appear oxidative and therefore metabolically capable of functioning during locomotion. The similar mitochondrial volumes and capillary densities of reptilian and mammalian muscles suggest that the greater oxidative capacity of mammalian muscle is due in part to possession of more oxidatively active mitochondria rather than to possession of more mitochondria per se.

  19. In situ X-ray fluorescence-based method to differentiate among red ochre pigments and yellow ochre pigments thermally transformed to red pigments of wall paintings from Pompeii.

    Science.gov (United States)

    Marcaida, Iker; Maguregui, Maite; Fdez-Ortiz de Vallejuelo, Silvia; Morillas, Héctor; Prieto-Taboada, Nagore; Veneranda, Marco; Castro, Kepa; Madariaga, Juan Manuel

    2017-06-01

    Most of the magnificent wall paintings from the ancient city of Pompeii are decorated with red and yellow colors coming from the ochre pigments used. The thermal impact of the pyroclastic flow from the eruption of Vesuvius, in AD 79, promoted the transformation of some yellow painted areas to red. In this work, original red ochre, original yellow ochre, and transformed yellow ochre (nowadays showing a red color) of wall paintings from Pompeian houses (House of Marcus Lucretius and House of Gilded Cupids) were analyzed by means of a handheld energy-dispersive X-ray fluorescence spectrometer to develop a fast method that allows chemical differentiation of the original red ochre and the transformed yellow ochre. Principal component analysis of the multivariate obtained data showed that arsenic is the tracer element to distinguish between both red colored ochres. Moreover, Pompeian raw red and yellow ochre pigments recovered from the burial were analyzed in the laboratory with use of a benchtop energy-dispersive X-ray fluorescence spectrometer to confirm the elemental composition and the conclusions drawn from the in situ analysis according to the yellow ochre pigment transformation in real Pompeian wall paintings.

  20. Aerobic methanotrophic communities at the Red Sea brine-seawater interface

    Directory of Open Access Journals (Sweden)

    Rehab Z. Abdallah

    2014-09-01

    Full Text Available The central rift of the Red Sea contains 25 brine pools with different physicochemical conditions, dictating the diversity and abundance of the microbial community. Three of these pools, the Atlantis II, Kebrit and Discovery Deeps, are uniquely characterized by a high concentration of hydrocarbons. The brine-seawater interface, described as an anoxic-oxic (brine-seawater boundary, is characterized by a high methane concentration, thus favoring aerobic methane oxidation. The current study analyzed the aerobic free–living methane-oxidizing bacterial communities that potentially contribute to methane oxidation at the brine-seawater interfaces of the three aforementioned brine pools, using metagenomic pyrosequencing, 16S rRNA pyrotags and pmoA library constructs. The sequencing of 16S rRNA pyrotags revealed that these interfaces are characterized by high microbial community diversity. Signatures of aerobic methane-oxidizing bacteria were detected in the Atlantis II Interface (ATII-I and the Kebrit Deep Upper (KB-U and Lower (KB-L brine-seawater interfaces. Through phylogenetic analysis of pmoA, we further demonstrated that the ATII-I aerobic methanotroph community is highly diverse. We propose four ATII-I pmoA clusters. Most importantly, cluster 2 groups with marine methane seep methanotrophs, and cluster 4 represent a unique lineage of an uncultured bacterium with divergent alkane monooxygenases. Moreover, non-metric multidimensional scaling (NMDS based on the ordination of putative enzymes involved in methane metabolism showed that the Kebrit interface layers were distinct from the ATII-I and DD-I brine-seawater interfaces.

  1. A single enhancer regulating the differential expression of duplicated red-sensitive opsin genes in zebrafish.

    Directory of Open Access Journals (Sweden)

    Taro Tsujimura

    2010-12-01

    Full Text Available A fundamental step in the evolution of the visual system is the gene duplication of visual opsins and differentiation between the duplicates in absorption spectra and expression pattern in the retina. However, our understanding of the mechanism of expression differentiation is far behind that of spectral tuning of opsins. Zebrafish (Danio rerio have two red-sensitive cone opsin genes, LWS-1 and LWS-2. These genes are arrayed in a tail-to-head manner, in this order, and are both expressed in the long member of double cones (LDCs in the retina. Expression of the longer-wave sensitive LWS-1 occurs later in development and is thus confined to the peripheral, especially ventral-nasal region of the adult retina, whereas expression of LWS-2 occurs earlier and is confined to the central region of the adult retina, shifted slightly to the dorsal-temporal region. In this study, we employed a transgenic reporter assay using fluorescent proteins and P1-artificial chromosome (PAC clones encompassing the two genes and identified a 0.6-kb "LWS-activating region" (LAR upstream of LWS-1, which regulates expression of both genes. Under the 2.6-kb flanking upstream region containing the LAR, the expression pattern of LWS-1 was recapitulated by the fluorescent reporter. On the other hand, when LAR was directly conjugated to the LWS-2 upstream region, the reporter was expressed in the LDCs but also across the entire outer nuclear layer. Deletion of LAR from the PAC clones drastically lowered the reporter expression of the two genes. These results suggest that LAR regulates both LWS-1 and LWS-2 by enhancing their expression and that interaction of LAR with the promoters is competitive between the two genes in a developmentally restricted manner. Sharing a regulatory region between duplicated genes could be a general way to facilitate the expression differentiation in duplicated visual opsins.

  2. The Fast-Casual Conundrum: Fast-Casual Restaurant Entrées Are Higher in Calories than Fast Food.

    Science.gov (United States)

    Schoffman, Danielle E; Davidson, Charis R; Hales, Sarah B; Crimarco, Anthony E; Dahl, Alicia A; Turner-McGrievy, Gabrielle M

    2016-10-01

    Frequently eating fast food has been associated with consuming a diet high in calories, and there is a public perception that fast-casual restaurants (eg, Chipotle) are healthier than traditional fast food (eg, McDonald's). However, research has not examined whether fast-food entrées and fast-casual entrées differ in calorie content. The purpose of this study was to determine whether the caloric content of entrées at fast-food restaurants differed from that found at fast-casual restaurants. This study was a cross-sectional analysis of secondary data. Calorie information from 2014 for lunch and dinner entrées for fast-food and fast-casual restaurants was downloaded from the MenuStat database. Mean calories per entrée between fast-food restaurants and fast-casual restaurants and the proportion of restaurant entrées that fell into different calorie ranges were assessed. A t test was conducted to test the hypothesis that there was no difference between the average calories per entrée at fast-food and fast-casual restaurants. To examine the difference in distribution of entrées in different calorie ranges between fast-food and fast-casual restaurants, χ(2) tests were used. There were 34 fast-food and 28 fast-casual restaurants included in the analysis (n=3,193 entrées). Fast-casual entrées had significantly more calories per entrée (760±301 kcal) than fast-food entrées (561±268; Pfast-casual entrées compared with fast-food entrées exceeded the median of 640 kcal per entrée (Pfast-casual entrées contained more calories than fast-food entrées in the study sample, future studies should compare actual purchasing patterns from these restaurants to determine whether the energy content or nutrient density of full meals (ie, entrées with sides and drinks) differs between fast-casual restaurants and fast-food restaurants. Calorie-conscious consumers should consider the calorie content of entrée items before purchase, regardless of restaurant type. Copyright

  3. Tong-xin-luo capsule inhibits left ventricular remodeling in spontaneously hypertensive rats by enhancing PPAR-γexpression and suppressing NF-κKB activity

    Institute of Scientific and Technical Information of China (English)

    BU Pei-li; ZHAO Xue-qiang; WANG Li-ling; ZHAO Yu-xia; LI Chuan-bao; ZHANG Yun

    2008-01-01

    Background Tong-xin-luo capsule(TXL),used as a traditional Chinese herb,offeres a therapeutic potential for treatment of cardiovascular diseases.It has been shown to exert a variety of pharmacological effects,including antihypertensiVe effects,and is able to improve ventricular remodeling.However,the mechanisms of its action are not completely understood.The aim of this study was to evaluate the molecular mechanisms of Tong-xin-luo capsule on left ventricular remodeling in spontaneously hypertensive rats (SHR).Methods Sixteen eight-week-old SHRs were randomized into an SHR group(n=8)and a TXL group(n=8)that were given Tong-xin-luo capsule(1.5 mg·kg-1·d-1).Eight Wistar Kyoto(WKY)rats fed with 0.9%NaCl served as the control group(WKY group).Systolic blood pressure(BP),body weight and heart rate were monitored once every two weeks.Ventricular remodeling was detected by hjstopathological examination.Nuclear factor kappa B P65(NF-κB P65)and peroxisome proliferators activated receptor γ(PPAR-γ)protein and phosphorylated inhibitor kappa α(IκBα)protein were detected by immunohistochemistry and western blot respectively.The physical interaction of the P65-P50 heterodimer with IκBα and NF-κB were measured by co-immunoprecipitation.PPAR-Y mRNA,collagen Ⅰ mRNA and collagenⅢ mHNA were measured by real-time PCR.Results TXL inhibited NF-κKB P65 expression and ventricular remodeling and suppressed the activation of NF-κB compared with the SHR group(P<0.01,P<0.05).TXL reduced IκBα phosphorylation,increased expression of PPAR-Yprotein and enhanced the physical interaction of the P65-P50 heterodimer with IκBα.The mRNA expression of PPAR-γwas enhanced but the mRNA expression of collagen Ⅰ mRNA and collagen Ⅲ mRNA were suppressed by TXL.Conclusions In spontaneously hypertensive rats,TXL could inhibit ventricular remodeling induced by hypertension,and the inhibitory effect might be associated with the process of TXL increasing the expression of PPAR-γ that

  4. Altered pathogenicity of a tl/CH/LDT3/03 genotype infectious bronchitis coronavirus due to natural recombination in the 5'- 17kb region of the genome.

    Science.gov (United States)

    Han, Zongxi; Zhang, Tingting; Xu, Qianqian; Gao, Mengying; Chen, Yuqiu; Wang, Qiuling; Zhao, Yan; Shao, Yuhao; Li, Huixin; Kong, Xiangang; Liu, Shengwang

    2016-02-02

    An infectious bronchitis coronavirus, designated as ck/CH/LGX/130530, was isolated from an IBV strain H120-vaccinated chicken in this study. Analysis of the S1 gene showed that isolate ck/CH/LGX/130530 was a tl/CH/LDT3/03-like virus, with a nucleotide sequence similarity of 99%. However, a complete genomic sequence analysis showed that ck/CH/LGX/130530 was more closely related to a Massachusetts type strain (95% similarity to strain H120) than to the tl/CH/LDT3/03 strain (86%), suggesting that recombination might have occurred during the origin of the virus. A SimPlot analysis of the complete genomic sequence confirmed this hypothesis, and it showed that isolate ck/CH/LGX/130530 emerged from a recombination event between parental IBV H120 strain and pathogenic tl/CH/LDT3/03-like virus. The results obtained from the pairwise comparison and nucleotide similarity showed that the recombination breakpoint was located in the nsp14 gene at nucleotides 17055-17083. In line with the high S1 gene sequence similarity, the ck/CH/LGX/130530 isolate was serotypically close to that of the tl/CH/LDT3/03 strain (73% antigenic relatedness). Furthermore, vaccination with the LDT3-A vaccine, which was derived from the tl/CH/LDT3/03 strain by serial passaging in chicken eggs, provided good protection against challenge with the tl/CH/LDT3/03 strain, in contrast to the poor protection offered with the H120 vaccine. Interestingly, isolate ck/CH/LGX/130530 exhibited low pathogenicity toward specific-pathogen-free chickens compared with the nephropathogenic tl/CH/LDT3/03 strain, which was likely due to natural recombination in the 5' 17-kb region of the genome. Our results also indicate that the replicase gene of IBV isolate ck/CH/LGX/130530 is associated with viral pathogenicity. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. The effect of a red leaf pigment on the relationship between red edge and chlorophyll concentration

    Science.gov (United States)

    Curran, Paul J.; Dungan, Jennifer L.; Macler, Bruce A.; Plummer, Stephen E.

    1991-01-01

    The effect of a leaf pigment - red amaranthin - on red edge and chlorophyll concentration is investigated in amaranth leaves by means of treatments with nitrate and salts. A near-linear relationship between red edge and chlorophyll concentration is observed for leaves with low amaranthin concentration, and no relationship is noted at high concentrations. The study demonstrates the limitation inherent in estimating chlorophyll concentration by using remotely sensed red edge.

  6. Red ginseng powder fermented with probiotics exerts antidiabetic effects in the streptozotocin-induced mouse diabetes model.

    Science.gov (United States)

    Jang, Sun-Hee; Park, Jisang; Kim, Sae-Hae; Choi, Kyung-Min; Ko, Eun-Sil; Cha, Jeong-Dan; Lee, Young-Ran; Jang, Hyonseok; Jang, Yong-Suk

    2017-12-01

    Red ginseng (heat-processed Panax ginseng) is a well-known alternative medicine with pharmacological antidiabetic activity. It exerts pharmacological effects through the transformation of saponin into metabolites by the intestinal microbiota. Given that intestinal conditions and intestinal microflora vary among individuals, the pharmacological effects of orally administered red ginseng likely may vary among individuals. To overcome this variation and produce homogeneously effective red ginseng, we evaluated the antidiabetic effects of probiotic-fermented red ginseng in a mouse model. The antidiabetic efficacy of orally administered probiotic-fermented red ginseng was assessed in ICR mice after induction of diabetes using streptozotocin (170 mg/kg body weight). Samples were given orally for 8 weeks, and indicators involved in diabetic disorders such as body weight change, water intake, blood glucose, glucose tolerance and various biochemical parameters were determined. Oral administration of probiotic-fermented red ginseng significantly decreased the level of blood glucose of about 62.5% in the fasting state and induced a significant increase in glucose tolerance of about 10.2% compared to the control diabetic mice. Additionally, various indicators of diabetes and biochemical data (e.g., blood glycosylated haemoglobin level, serum concentrations of insulin, and α-amylase activity) showed a significant improvement in the diabetic conditions of the mice treated with probiotic-fermented red ginseng in comparison with those of control diabetic mice. Our results demonstrate the antidiabetic effects of probiotic-fermented red ginseng in the streptozotocin-induced mouse diabetes model and suggest that probiotic-fermented red ginseng may be a uniformly effective red ginseng product.

  7. Asteroseismology of Red Giant stars

    CERN Document Server

    Tarrant, N J; Elsworth, Y P; Spreckley, S A; Stevens, I R

    2008-01-01

    Sun-like oscillations, that is p-modes excited stochastically by convective noise, have now been observed in a number of Red Giant stars. Compared to those seen in the Sun, these modes are of large amplitude and long period, making the oscillations attractive prospects for observation. However, the low Q-factor of these modes, and issues relating to the rising background at low frequencies, present some interesting challenges for identifying modes and determining the related asteroseismic parameters. We report on the analysis procedure adopted for peak-bagging by our group at Birmingham, and the techniques used to robustly ensure these are not a product of noise. I also show results from a number of giants extracted from multi-year observations with the SMEI instrument.

  8. Voyager 1 Red Spot Movie

    Science.gov (United States)

    2000-01-01

    This movie shows the portion of Jupiter around the Great Red Spot as it swirls through more than 60 Jupiter days. Notice the difference in speed and direction of the various zones of the atmosphere. The interaction of the atmospheric clouds and storm shows how dynamic the Jovian atmosphere is.As Voyager 1 approached Jupiter in 1979, it took images of the planet at regular intervals. This sequence is made from 66 images taken once every Jupiter rotation period (about 10 hours). This time-lapse movie uses images taken every time Jupiter longitude 68W passed under the spacecraft. These images were acquired in the Blue filter from Jan. 6 to Feb. 3 1979. The spacecraft flew from 58 million kilometers to 31 million kilometers from Jupiter during that time.This time-lapse movie was produced at JPL by the Image Processing Laboratory in 1979.

  9. Control of poultry red mites

    DEFF Research Database (Denmark)

    Kilpinen, Ole; Steenberg, Tove

    2008-01-01

    The poultry red mite (PRM), Dermanyssus gallinae, is the most important ectoparasite in European egg production. The mites hide in cracks and crevices in the near vicinity of the resting places of the birds, coming out to feed mainly during the night. Under favourable conditions the population can...... grow rapidly, leading to serious problems. Large mite populations may cause anaemia or even death to the poultry, but also in lower numbers mites may be a nuisance to the birds causing decreased egg production and egg quality. Furthermore, they may have the potential of acting as reservoir....../carriers for various micro-organisms e.g. Salmonella. In SAFEHOUSE, an EU-project running from 2006-2009, partners from 11 European countries aim at developing new methods for prevention and control of Salmonella in egg production systems with particular focus on the transition to enriched cages in the EU. Enriched...

  10. BIOSORPTION OF CONGO RED BY HYDROGEN PEROXIDE TREATED TENDU WASTE

    Directory of Open Access Journals (Sweden)

    G. K. Nagda ، V. S. Ghole

    2009-07-01

    Full Text Available Solid wastes from agro-industrial operations can be recycled as non-conventional adsorbents if they are inert and harmless and reduce the cost of wastewater treatment. Tendu leaf Diospyros melanoxylon is the second largest forest product in India after timber and is exclusively used in making local cigarette called Bidi. Waste leaf cutting remaining after making cigarette was used in present study as a biosorbent for the removal of Congo red dye from aqueous solution. It was treated with hydrogen peroxide to obtain biosorbent with increased adsorption capacity. Batch type experiments were conducted to study the influence of different parameters such as pH, initial dye concentration and dosage of adsorbent on biosorption evaluated. The adsorption occured very fast initially and attains equilibrium within 60 min at pH= 6.2 and the equilibrium attained faster after hydrogen peroxide modification. Kinetic studies showed that the biosorption of Congo red on tendu waste followed pseudo-second-order rate equation. The data fitted well to Langmuir and Freundlich isotherm models. Comparison was done on the extent of biosorption between untreated and treated forms of the tendu waste. The maximum adsorption capacity for untreated tendu waste was found to be 46.95 mg/g, which was enhanced by 2.8 times after hydrogen peroxide treatment and was found to be 134.4 mg/g. The adsorption process was in conformity with Freundlich and Langmuir isotherms for Congo red adsorption from aqueous solution. The study demonstrated use of milder chemical treatment of tendu waste to obtain a biosorbent with enhanced dye removal capacity.

  11. The acute stress response of red porgy, Pagrus pagrus, kept on a red or white background

    NARCIS (Netherlands)

    Salm, A.L. van der; Pavlidis, M.; Flik, G.; Wendelaar Bonga, S.E.

    2005-01-01

    The skin colour of red porgy, Pagrus pagrus, can be modified by exposure to different background colours. Red and white background colours brighten the dark skin colour that develops under common culture conditions in red porgy. To assess whether skin colour is also modified by aquaculture related

  12. The acute stress response of red porgy, Pagrus pagrus, kept on a red or white background

    NARCIS (Netherlands)

    Salm, A.L. van der; Pavlidis, M.; Flik, G.; Wendelaar Bonga, S.E.

    2005-01-01

    The skin colour of red porgy, Pagrus pagrus, can be modified by exposure to different background colours. Red and white background colours brighten the dark skin colour that develops under common culture conditions in red porgy. To assess whether skin colour is also modified by aquaculture related h

  13. Two thyroid hormone regulated genes, the beta-subunits of nerve growth factor (NGFB) and thyroid stimulating hormone (TSHB), are located less than 310 kb apart in both human and mouse genomes.

    Science.gov (United States)

    Dracopoli, N C; Rose, E; Whitfield, G K; Guidon, P T; Bale, S J; Chance, P A; Kourides, I A; Housman, D E

    1988-08-01

    Two thyroid hormone regulated genes, the beta-subunits of nerve growth factor (NGFB) and thyroid stimulating hormone (TSHB), have been assigned to mouse chromosome 3 and human chromosome 1p22. We have used the techniques of linkage analysis and pulsed field gel electrophoresis to determine the proximity of these two antithetically regulated genes in this conserved linkage group. Four novel restriction fragment length polymorphisms were identified at the human TSHB gene. Two-point linkage analysis between TSHB and NGFB in 46 families, including the Centre d'Etude du Polymorphisme Humain (CEPH) reference panel, demonstrated no recombination (theta = 0.00, Z = 42.8). Analysis of this region by pulsed field gel electrophoresis showed that the genes for TSHB and NGFB are located less than 310 kb apart in man and 220 kb in the mouse.

  14. [Quality management in a combined clinic - the quality management system according to DIN EN ISO 9001 of the The German Association of Spa Accommodation Resorts e. V. (VdKB)].

    Science.gov (United States)

    Eckert, H; Schulze, U

    2004-06-01

    The implementation of a quality system in health care organizations offers huge potentials for improvement and development. But the realization of such systems necessitates high investments, which small companies and family businesses often are unable to raise. The German Association of Spa Accommodation Resorts (VdKB) commissioned two consultants to develop a reasonably priced implementation concept of a DIN EN ISO 9001 quality system specially adjusted to small companies and family businesses in medical spa and rehabilitation services, which was successfully accredited in May 2003. This paper describes fundamental requirements in combine management systems, considers the pros and cons of management combines and demonstrates the practical realization of combine requirements on the example of the VdKB-combine system.

  15. [Preoperative fasting guidelines: an update].

    Science.gov (United States)

    López Muñoz, A C; Busto Aguirreurreta, N; Tomás Braulio, J

    2015-03-01

    Anesthesiology societies have issued various guidelines on preoperative fasting since 1990, not only to decrease the incidence of lung aspiration and anesthetic morbidity, but also to increase patient comfort prior to anesthesia. Some of these societies have been updating their guidelines, as such that, since 2010, we now have 2 evidence-based preoperative fasting guidelines available. In this article, an attempt is made to review these updated guidelines, as well as the current instructions for more controversial patients such as infants, the obese, and a particular type of ophthalmic surgery. Copyright © 2014 Sociedad Española de Anestesiología, Reanimación y Terapéutica del Dolor. Publicado por Elsevier España, S.L.U. All rights reserved.

  16. A Repeating Fast Radio Burst

    CERN Document Server

    Spitler, L G; Hessels, J W T; Bogdanov, S; Brazier, A; Camilo, F; Chatterjee, S; Cordes, J M; Crawford, F; Deneva, J; Ferdman, R D; Freire, P C C; Kaspi, V M; Lazarus, P; Lynch, R; Madsen, E C; McLaughlin, M A; Patel, C; Ransom, S M; Seymour, A; Stairs, I H; Stappers, B W; van Leeuwen, J; Zhu, W W

    2016-01-01

    Fast Radio Bursts are millisecond-duration astronomical radio pulses of unknown physical origin that appear to come from extragalactic distances. Previous follow-up observations have failed to find additional bursts at the same dispersion measures (i.e. integrated column density of free electrons between source and telescope) and sky position as the original detections. The apparent non-repeating nature of the fast radio bursts has led several authors to hypothesise that they originate in cataclysmic astrophysical events. Here we report the detection of ten additional bursts from the direction of FRB121102, using the 305-m Arecibo telescope. These new bursts have dispersion measures and sky positions consistent with the original burst. This unambiguously identifies FRB121102 as repeating and demonstrates that its source survives the energetic events that cause the bursts. Additionally, the bursts from FRB121102 show a wide range of spectral shapes that appear to be predominantly intrinsic to the source and wh...

  17. Spherical Demons: Fast Surface Registration

    Science.gov (United States)

    Yeo, B.T. Thomas; Sabuncu, Mert; Vercauteren, Tom; Ayache, Nicholas; Fischl, Bruce; Golland, Polina

    2009-01-01

    We present the fast Spherical Demons algorithm for registering two spherical images. By exploiting spherical vector spline interpolation theory, we show that a large class of regularizers for the modified demons objective function can be efficiently implemented on the sphere using convolution. Based on the one parameter subgroups of diffeomorphisms, the resulting registration is diffeomorphic and fast – registration of two cortical mesh models with more than 100k nodes takes less than 5 minutes, comparable to the fastest surface registration algorithms. Moreover, the accuracy of our method compares favorably to the popular FreeSurfer registration algorithm. We validate the technique in two different settings: (1) parcellation in a set of in-vivo cortical surfaces and (2) Brodmann area localization in ex-vivo cortical surfaces. PMID:18979813

  18. ATLAS FTK: The Fast Tracker

    CERN Document Server

    T, Iizawa; The ATLAS collaboration

    2014-01-01

    The Fast TracKer (FTK) will perform global track reconstruction after each Level-1 trigger accept to enable the software-based High Level Trigger to have early access to tracking information. FTK is a dedicated processor based on a mixture of advanced technologies. Modern, powerful Field Programmable Gate Arrays (FPGAs) form an important part of the system architecture, and the large level of computing power required for pattern recognition is provided by incorporating standard-cell ASICs named Associative Memory (AM). FTK provides global track reconstruction in the full inner silicon detector in approximately 100 microseconds with resolution comparable to the offline algorithms. It allows a fast and precise detection of the primary and secondary vertex information. The track and vertex information is then used by the High Level Trigger algorithms, allowing highly improved trigger performance for the important signatures such as b-jets. In this paper, the architecture and the hardware development status of FT...

  19. Ultra-fast silicon detectors

    Energy Technology Data Exchange (ETDEWEB)

    Sadrozinski, H. F.-W., E-mail: hartmut@scipp.ucsc.edu [Santa Cruz Institute for Particle Physics, UC Santa Cruz, Santa Cruz, CA 95064 (United States); Ely, S.; Fadeyev, V.; Galloway, Z.; Ngo, J.; Parker, C.; Petersen, B.; Seiden, A.; Zatserklyaniy, A. [Santa Cruz Institute for Particle Physics, UC Santa Cruz, Santa Cruz, CA 95064 (United States); Cartiglia, N.; Marchetto, F. [INFN Torino, Torino (Italy); Bruzzi, M.; Mori, R.; Scaringella, M.; Vinattieri, A. [University of Florence, Department of Physics and Astronomy, Sesto Fiorentino, Firenze (Italy)

    2013-12-01

    We propose to develop a fast, thin silicon sensor with gain capable to concurrently measure with high precision the space (∼10 μm) and time (∼10 ps) coordinates of a particle. This will open up new application of silicon detector systems in many fields. Our analysis of detector properties indicates that it is possible to improve the timing characteristics of silicon-based tracking sensors, which already have sufficient position resolution, to achieve four-dimensional high-precision measurements. The basic sensor characteristics and the expected performance are listed, the wide field of applications are mentioned and the required R and D topics are discussed. -- Highlights: •We are proposing thin pixel silicon sensors with 10's of picoseconds time resolution. •Fast charge collection is coupled with internal charge multiplication. •The truly 4-D sensors will revolutionize imaging and particle counting in many applications.

  20. Fast Moreau envelope computation I

    Science.gov (United States)

    Lucet, Yves

    2006-11-01

    The present article summarizes the state of the art algorithms to compute the discrete Moreau envelope, and presents a new linear-time algorithm, named NEP for NonExpansive Proximal mapping. Numerical comparisons between the NEP and two existing algorithms: The Linear-time Legendre Transform (LLT) and the Parabolic Envelope (PE) algorithms are performed. Worst-case time complexity, convergence results, and examples are included. The fast Moreau envelope algorithms first factor the Moreau envelope as several one-dimensional transforms and then reduce the brute force quadratic worst-case time complexity to linear time by using either the equivalence with Fast Legendre Transform algorithms, the computation of a lower envelope of parabolas, or, in the convex case, the non expansiveness of the proximal mapping.

  1. A fast meteor detection algorithm

    Science.gov (United States)

    Gural, P.

    2016-01-01

    A low latency meteor detection algorithm for use with fast steering mirrors had been previously developed to track and telescopically follow meteors in real-time (Gural, 2007). It has been rewritten as a generic clustering and tracking software module for meteor detection that meets both the demanding throughput requirements of a Raspberry Pi while also maintaining a high probability of detection. The software interface is generalized to work with various forms of front-end video pre-processing approaches and provides a rich product set of parameterized line detection metrics. Discussion will include the Maximum Temporal Pixel (MTP) compression technique as a fast thresholding option for feeding the detection module, the detection algorithm trade for maximum processing throughput, details on the clustering and tracking methodology, processing products, performance metrics, and a general interface description.

  2. Le logiciel FastCart

    OpenAIRE

    1986-01-01

    FastCart est un logiciel destiné à l'enseignement de la géographie. Il permet aux élèves de créer leurs propres documents d'études: histogrammes, cartes en hachures, cartes de classification de l'espace. Sa rapidité d'exécution et sa facilité d'utilisation en font un outil adapté aux exigences des enseignants.

  3. The FastGas detector

    Energy Technology Data Exchange (ETDEWEB)

    Bateman, J.E.; Dalgliesh, R.M. [Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0QX (United Kingdom); Duxbury, D.M., E-mail: dom.duxbury@stfc.ac.u [Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0QX (United Kingdom); Holt, S.A.; McPhail, D.J. [Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0QX (United Kingdom); Marsh, A.S. [Diamond Light Source LTD, Harwell Science and Innovation Campus, Diamond House, Chilton, Didcot, Oxfordshire, OX11 0DE (United Kingdom); Rhodes, N.J.; Schooneveld, E.M.; Spill, E.J.; Stephenson, R. [Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0QX (United Kingdom)

    2010-04-21

    The development and testing of the FastGas neutron detector is described. Based on a Gas Microstrip Chamber the aim of the project was to produce a high counting rate detector capable of replacing the existing {sup 3}He tubes for specular reflectometry, currently in use on the ISIS reflectometer instruments. The detector system is described together with results of neutron beam tests carried out at the ISIS spallation neutron source.

  4. Instrumentation of the fast detector

    CERN Document Server

    Barczyk, A.; Malgeri, L.; Casella, C.; Pohl, M.; Deiters, K.; Dick, P.; Berdugo, J.; Casaus, J.; Mana, C.; Marin, J.; Martinez, G.; Sanchez, E.; Willmott, C.

    2008-01-01

    The Fiber Active Scintillator Target (FAST) is an imaging particle detector intended for high precision muon lifetime measurement. This measurement will lead to a determination of the Fermi coupling constant (GF) with an uncertainty of 1 ppm, one order of magnitude better than the current world average. This contribution presents a description of the detector instrumentation and the first results, which have validated the design of the system.

  5. Programmable fast data acquisition system

    Science.gov (United States)

    Montebugnoli, S.; Bianchi, G.; Zoni, L.

    The goal of this work is to investigate the possibility to install on modern radiotelescopes a fast and programmable backend instead of several backends, each dedicated to a single acquisition task. Such an approach could lower the costs and make available a more compact and flexible back end. Exploiting the state-of-the-art of the FPGAs (Field Programmable Gate Arrays) and innovative architectures, a programmable system might be conceived.

  6. Fast regularized image interpolation method

    Institute of Scientific and Technical Information of China (English)

    Hongchen Liu; Yong Feng; Linjing Li

    2007-01-01

    The regularized image interpolation method is widely used based on the vector interpolation model in which down-sampling matrix has very large dimension and needs large storage consumption and higher computation complexity. In this paper, a fast algorithm for image interpolation based on the tensor product of matrices is presented, which transforms the vector interpolation model to matrix form. The proposed algorithm can extremely reduce the storage requirement and time consumption. The simulation results verify their validity.

  7. Reversal of P-gp and MRP1-mediated multidrug resistance by H6, a gypenoside aglycon from Gynostemma pentaphyllum, in vincristine-resistant human oral cancer (KB/VCR) cells.

    Science.gov (United States)

    Zhu, Hengrui; Liu, Zulong; Tang, Lisha; Liu, Junhua; Zhou, Mei; Xie, Fang; Wang, Zheng; Wang, Yuqi; Shen, Sida; Hu, Lihong; Yu, Long

    2012-12-05

    Multidrug resistance (MDR) to anticancer drugs is a major obstacle to successful chemotherapy in the treatment of cancers. Identification of natural compounds capable of circumventing MDR with minimal adverse side effects is an attractive goal. Here, we found that H6, a gypenoside aglycon from Gynostemma pentaphyllum, displayed potent anti-MDR activity. Average resistant fold (RF) of H6 is 1.03 and 1.04 in KB/VCR and MCF-7/ADR cells compared to their parental cells. H6 alone ranging from 2 μmol/l to 40 μmol/l (μM) did not display a significant anti-proliferative effect on KB/VCR cells and other cells, while the compound at these concentrations enhanced the cytotoxicity of vincristine (VCR) to KB/VCR cells. H6 showed a significant synergistic effect in combination with VCR. By quantification of sub-G(1) fraction cells, H6 also enhanced the VCR-induced apoptosis in a dose-dependent manner. The short time treatment with H6 increased the intracellular accumulation of rhodamine 123 (Rho123) and 5(6)-carboxyfluorescein diacetate (CFDA) in KB/VCR cells. Further studies showed that H6 treatment resulted in the decrease of the RNA transcript level of P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1) and breast cancer resistance protein (BCRP). H6 inhibited the function of P-gp by stimulating P-gp ATPase activity and decreased MRP1 expression with a blockade of STAT3 phosphorylation. These findings suggest that H6, a multi-targets reversal agent with no significant toxic effect, may be a potential candidate to circumvent the P-gp and MRP1-mediated MDR. Copyright © 2012 Elsevier B.V. All rights reserved.

  8. La red sobre trabajo infantil peligroso (Red Tip The hazardous child labor network (Red Tip

    Directory of Open Access Journals (Sweden)

    Walter Varillas

    2003-01-01

    Full Text Available En el mundo, aproximadamente 351.7 millones de niños entre 5 y 17 años realizaban algún tipo de actividad económica, de ellos 170.5 millones (48.5% realizaban algún tipo de trabajo considerado peligroso. Un alto porcentaje se encuentra en la agricultura, otros en minas, manufacturas, ladrilleras, predominantemente en la economía informal. El Convenio 138 (cobre la edad mínima de admisión en el empleo de la OIT y el Convenio 182 (sobre las peores formas de trabajo infantil, definen como trabajo infantil peligroso el que puede afectar la salud, seguridad y moralidad de los menores. Estudios específicos sobre los menores muestran su susceptibilidad particular frente a los riesgos laborales, aumentando la peligrosidad para su normal desarrollo y crecimiento: "los niños no son adultos pequeños". Los profesionales de la seguridad y salud en el trabajo pueden colaborar con los profesionales y las organizaciones especializadas en el trabajo infantil, en la definición y caracterización de lo que significa el trabajo infantil peligroso. Para ello se ha conformado la Red sobre Trabajo Infantil Peligroso (Red TIP, con la finalidad de articular estos dos espacios, orientados a eliminar el trabajo infantil peligroso y rescatar al menor y devolverle la oportunidad de sonreír ahora y en el futuro.In the world, approximately 351.7 millions children between 5 and 17 years old work. Of them, 170,5 millions (48.5% work at the hazardous child labor forms. A high percentage is in agriculture, others in mines, manufactures, brick makers, predominantly in informal economy. The 138 Convention of ILO and the 182 Convention, define as hazardous child labor activities that can affect the health, safety and morality of the children. Studies on the children at work point out their particular susceptibility to some occupational risks, increasing the danger for their normal development and growth. "They are not little adults". The occupational health

  9. ATLAS Fast Physics Monitoring: TADA

    CERN Document Server

    Elsing, Markus; The ATLAS collaboration; Sabato, Gabriele; Kamioka, Shusei; Nairz, Armin Michael; Moyse, Edward; Gumpert, Christian

    2016-01-01

    The ATLAS Experiment at the LHC is recording data from proton-proton collisions with 13 TeV center-of-mass energy since spring 2015. The collaboration is using a fast physics monitoring framework (TADA) to automatically perform a broad range of fast searches for early signs of new physics and to monitor the data quality across the year with the full analysis level calibrations applied to the rapidly growing data. TADA is designed to provide fast feedback directly after the collected data has been fully calibrated and processed at the Tier-0. The system can monitor a large range of physics channels, offline data quality and physics performance quantities nearly final analysis level object calibrations. TADA output is available on a website accessible by the whole collaboration that gets updated twice a day with the data from newly processed runs. Hints of potentially interesting physics signals or performance issues identified in this way are reported to be followed up by physics or combined performance groups...

  10. ATLAS Fast Physics Monitoring: TADA

    CERN Document Server

    Sabato, Gabriele; The ATLAS collaboration

    2017-01-01

    The ATLAS experiment at the LHC is recording data from proton-proton collisions with 13 TeV center-of-mass energy since spring 2015. The collaboration is using a fast physics monitoring framework (TADA) to automatically perform a broad range of fast searches for early signs of new physics and to monitor the data quality across the year with the full analysis level calibrations applied to the rapidly growing data.TADA is designed to provide fast feedback directly after the collected data has been fully calibrated and processed at the Tier-0, the CERN Data Center. The system can monitor a large range of physics channels, offline data quality and physics performance quantities nearly final analysis level object calibrations. TADA output is available on a website accessible by the whole collaboration that gets updated twice a day with the data from newly processed runs. Hints of potentially interesting physics signals or performance issues identified in this way are reported to be followed up by physics or combin...

  11. Heterogeneous Transmutation Sodium Fast Reactor

    Energy Technology Data Exchange (ETDEWEB)

    S. E. Bays

    2007-09-01

    The threshold-fission (fertile) nature of Am-241 is used to destroy this minor actinide by capitalizing upon neutron capture instead of fission within a sodium fast reactor. This neutron-capture and its subsequent decay chain leads to the breeding of even neutron number plutonium isotopes. A slightly moderated target design is proposed for breeding plutonium in an axial blanket located above the active “fast reactor” driver fuel region. A parametric study on the core height and fuel pin diameter-to-pitch ratio is used to explore the reactor and fuel cycle aspects of this design. This study resulted in both non-flattened and flattened core geometries. Both of these designs demonstrated a high capacity for removing americium from the fuel cycle. A reactivity coefficient analysis revealed that this heterogeneous design will have comparable safety aspects to a homogeneous reactor of comparable size. A mass balance analysis revealed that the heterogeneous design may reduce the number of fast reactors needed to close the current once-through light water reactor fuel cycle.

  12. Red blood cell alloimmunization after blood transfusion

    NARCIS (Netherlands)

    Schonewille, Henk

    2008-01-01

    Current pretransfusion policy requires the patients’ serum to be tested for the presence of irregular red blood cell antibodies. In case of an antibody, red blood cells lacking the corresponding antigen are transfused after an antiglobulin crossmatch. The aim of the studies in this thesis is primari

  13. Internal rotation of red giants by asteroseismology

    CERN Document Server

    Di Mauro, M P; Ventura, R; Stello, D; Beck, P G; Davies, G; Elsworth, Y; Garcıa, R A; Hekker, S; Mosser, B; Christensen-Dalsgaard, J; Bloemen, S; Catanzaro, G; De Smedt, K; Tkachenko, A

    2012-01-01

    We present an asteroseismic approach to study the dynamics of the stellar interior in red-giant stars by asteroseismic inversion of the splittings induced by the stellar rotation on the oscillation frequencies. We show preliminary results obtained for the red giant KIC4448777 observed by the space mission Kepler.

  14. Red grain mycetoma foot in Western Rajasthan

    Directory of Open Access Journals (Sweden)

    Mathur D

    1994-01-01

    Full Text Available Usually the colour of the grains seen in cases of mycetoma are either black or yellow. Recently there were reports that unusual red grains had been noticed in cases of mycetoma. A case of red grain mycetoma is reported.

  15. The Austrian x red pine hybrid

    Science.gov (United States)

    W. B. Critchfield

    1963-01-01

    The genetic improvement of red pine (Pinus resinosa Ait.) presents tree breeders with one of their most difficult problems. Not only is this valuable species remarkably uniform, but until 1955 it resisted all attempts to cross it with other pines. In that year red pine and Austrian pine (P. nigra var. austriaca [...

  16. Red autofluorescence of dental plaque bacteria

    NARCIS (Netherlands)

    van der Veen, M. H.; Thomas, R. Z.; Huysmans, M. C. D. N. J. M.; de Soet, J. J.

    2006-01-01

    Red autofluorescence of plaque and its relation to fluorescence of a single species in the biofilm was studied. Fluorescence images of non-disclosed and disclosed plaque of 28 first-year students were captured. The plaque samples were assessed by culture methods and studied for red autofluorescence.

  17. Experimental reintroduction of red-cockaded woodpeckers

    Science.gov (United States)

    D. Craig Rudolph; Richard N. Conner; Dawn K. Carrie; Richard R. Schaefer

    1992-01-01

    The Red-cockaded Woodpecker (Picoides borealis) is an endangered species endemic to the pine forests of the southeastern United States (Jackson 1971). Deforestation and habitat alteration have severely affected Red-cockaded Woodpecker populations; current populations are isolated and most are declining (Jackson 1971, Lennartz et al. 1983, Conner and Rudolph 1989, Costa...

  18. Phosphate removal from wastewater using red mud.

    Science.gov (United States)

    Huang, Weiwei; Wang, Shaobin; Zhu, Zhonghua; Li, Li; Yao, Xiangdong; Rudolph, Victor; Haghseresht, Fouad

    2008-10-01

    Red mud, a waste residue of alumina refinery, has been used to develop effective adsorbents to remove phosphate from aqueous solution. Acid and acid-thermal treatments were employed to treat the raw red mud. The effects of different treatment methods, pH of solution and operating temperature on adsorption have been examined in batch experiments. It was found that all activated red mud samples show higher surface area and total pore volume as well as higher adsorption capacity for phosphate removal. The red mud with HCl treatment shows the highest adsorption capacity among all the red mud samples, giving adsorption capacity of 0.58 mg P/g at pH 5.5 and 40 degrees C. The adsorption capacity of the red mud adsorbents decreases with increase of pH. At pH 2, the red mud with HCl treatment exhibits adsorption of 0.8 mg P/g while the adsorption can be lowered to 0.05 mg P/g at pH 10. However, the adsorption is improved at higher temperature by increasing 25% from 30 to 40 degrees C. The kinetic studies of phosphate adsorption onto red mud indicate that the adsorption mainly follows the parallel first-order kinetics due to the presence of two acidic phosphorus species, H(2)PO(4)(-) and HPO(4)(2-). An analysis of the adsorption data indicates that the Freundlich isotherm provides a better fitting than the Langmuir model.

  19. 21 CFR 640.10 - Red Blood Cells.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Red Blood Cells. 640.10 Section 640.10 Food and... ADDITIONAL STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Red Blood Cells § 640.10 Red Blood Cells. The proper name of this product shall be Red Blood Cells. The product is defined as red blood cells remaining...

  20. White dwarf-red dwarf binaries in the Galaxy

    NARCIS (Netherlands)

    Besselaar, E.J.M. van den

    2007-01-01

    This PhD thesis shows several studies on white dwarf - red dwarf binaries. White dwarfs are the end products of most stars and red dwarfs are normal hydrogen burning low-mass stars. White dwarf - red dwarf binaries are both blue (white dwarf) and red (red dwarf). Together with the fact that they are