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Sample records for factors showed significant

  1. Coagulation tests show significant differences in patients with breast cancer.

    Science.gov (United States)

    Tas, Faruk; Kilic, Leyla; Duranyildiz, Derya

    2014-06-01

    Activated coagulation and fibrinolytic system in cancer patients is associated with tumor stroma formation and metastasis in different cancer types. The aim of this study is to explore the correlation of blood coagulation assays for various clinicopathologic factors in breast cancer patients. A total of 123 female breast cancer patients were enrolled into the study. All the patients were treatment naïve. Pretreatment blood coagulation tests including PT, APTT, PTA, INR, D-dimer, fibrinogen levels, and platelet counts were evaluated. Median age of diagnosis was 51 years old (range 26-82). Twenty-two percent of the group consisted of metastatic breast cancer patients. The plasma level of all coagulation tests revealed statistically significant difference between patient and control group except for PT (p50 years) was associated with higher D-dimer levels (p=0.003). Metastatic patients exhibited significantly higher D-dimer values when compared with early breast cancer patients (p=0.049). Advanced tumor stage (T3 and T4) was associated with higher INR (p=0.05) and lower PTA (p=0.025). In conclusion, coagulation tests show significant differences in patients with breast cancer.

  2. Significance evaluation in factor graphs

    DEFF Research Database (Denmark)

    Madsen, Tobias; Hobolth, Asger; Jensen, Jens Ledet

    2017-01-01

    Background Factor graphs provide a flexible and general framework for specifying probability distributions. They can capture a range of popular and recent models for analysis of both genomics data as well as data from other scientific fields. Owing to the ever larger data sets encountered...... in genomics and the multiple-testing issues accompanying them, accurate significance evaluation is of great importance. We here address the problem of evaluating statistical significance of observations from factor graph models. Results Two novel numerical approximations for evaluation of statistical....... Conclusions The applicability of saddlepoint approximation and importance sampling is demonstrated on known models in the factor graph framework. Using the two methods we can substantially improve computational cost without compromising accuracy. This contribution allows analyses of large datasets...

  3. Human-directed social behaviour in dogs shows significant heritability.

    Science.gov (United States)

    Persson, M E; Roth, L S V; Johnsson, M; Wright, D; Jensen, P

    2015-04-01

    Through domestication and co-evolution with humans, dogs have developed abilities to attract human attention, e.g. in a manner of seeking assistance when faced with a problem solving task. The aims of this study were to investigate within breed variation in human-directed contact seeking in dogs and to estimate its genetic basis. To do this, 498 research beagles, bred and kept under standardized conditions, were tested in an unsolvable problem task. Contact seeking behaviours recorded included both eye contact and physical interactions. Behavioural data was summarized through a principal component analysis, resulting in four components: test interactions, social interactions, eye contact and physical contact. Females scored significantly higher on social interactions and physical contact and age had an effect on eye contact scores. Narrow sense heritabilities (h(2) ) of the two largest components were estimated at 0.32 and 0.23 but were not significant for the last two components. These results show that within the studied dog population, behavioural variation in human-directed social behaviours was sex dependent and that the utilization of eye contact seeking increased with age and experience. Hence, heritability estimates indicate a significant genetic contribution to the variation found in human-directed social interactions, suggesting that social skills in dogs have a genetic basis, but can also be shaped and enhanced through individual experiences. This research gives the opportunity to further investigate the genetics behind dogs' social skills, which could also play a significant part into research on human social disorders such as autism.

  4. This research is to study the factors which influence the business success of small business ‘processed rotan’. The data employed in the study are primary data within the period of July to August 2013, 30 research observations through census method. Method of analysis used in the study is multiple linear regressions. The results of analysis showed that the factors of labor, innovation and promotion have positive and significant influence on the business success of small business ‘processed rotan’ simultaneously. The analysis also showed that partially labor has positive and significant influence on the business success, yet innovation and promotion have insignificant and positive influence on the business success.

    OpenAIRE

    Nasution, Inggrita Gusti Sari; Muchtar, Yasmin Chairunnisa

    2013-01-01

    This research is to study the factors which influence the business success of small business ‘processed rotan’. The data employed in the study are primary data within the period of July to August 2013, 30 research observations through census method. Method of analysis used in the study is multiple linear regressions. The results of analysis showed that the factors of labor, innovation and promotion have positive and significant influence on the business success of small busine...

  5. EXPLORE SIGNIFICANT FACTORS TO AFFECT CUSTOMER INVOLVEMENT

    Directory of Open Access Journals (Sweden)

    Yu-Jia Hu

    2012-01-01

    Full Text Available Although literature review supported the concept that customer loyalty, brand equity and perceived risk are significant factors to affect customer involvement, very limited studies have extensively examined the relationship among those variables. This research applied quantitative study to comprehensively explore the relationship between customer loyalty, brand equity, perceived risk and customer involvement for consumers. The population for this research was identified as consumers having the shopping experience for digital camera. The findings supported the hypothesis that customer loyalty, brand equity and perceived risk have significant and positive relationship to customer involvement. The findings identified the predictors of customer loyalty, brand equity and perceived risk on the customer involvement and generated the recommendations for corporate operations and future scholar studies.

  6. Representativeness and significance factors in ESP texts

    Directory of Open Access Journals (Sweden)

    Alejandro Curado Fuentes

    2000-04-01

    Full Text Available The development of communicative approaches and strategies in specialized discourse has led to revising notions of representative and significant language . Particularly in the work with academic genres, in science and technology (EST settings such as our own institution, the need for determining these factors is ever growing. The application of empirical resources such as specific language corpora, in fact, becomes convenient. In this paper, the aim is to specify the type of corpus linguistic representativeness and significance sought in the case of teaching English to our groups of Computer Science students. In that scope, we present data and samples on which to base our suggestions and claims regarding the exploitation of textual material.

  7. Fungal communities in wheat grain show significant co-existence patterns among species

    DEFF Research Database (Denmark)

    Nicolaisen, M.; Justesen, A. F.; Knorr, K.;

    2014-01-01

    identified as ‘core’ OTUs as they were found in all or almost all samples and accounted for almost 99 % of all sequences. The remaining OTUs were only sporadically found and only in small amounts. Cluster and factor analyses showed patterns of co-existence among the core species. Cluster analysis grouped...

  8. PCOS women show significantly higher homocysteine level, independent to glucose and E2 level

    Science.gov (United States)

    Eskandari, Zahra; Sadrkhanlou, Rajab-Ali; Nejati, Vahid; Tizro, Gholamreza

    2016-01-01

    Background: It is reasonable to think that some biochemical characteristics of follicular fluid (FF) surrounding the oocyte may play a critical role in determining the quality of oocyte and the subsequent potential needed to achieve fertilization and embryo development. Objective: This study was carried out to evaluate the levels of FF homocysteine (Hcy) in IVF candidate polycystic ovary syndrome (PCOS) women and any relationships with FF glucose and estradiol (E2) levels. Materials and Methods: In this case control study which was performed in Dr. Tizro Day Care and IVF Center 70 infertile patients were enrolled in two groups: comprising 35 PCOS and 35 non PCOS women. Long protocol was performed for all patients. FF Hcy, glucose and E2 levels were analyzed at the time of oocyte retrieval. Results: It was observed that FF Hcy level was significantly higher in PCOS patients compared with non PCOSs (p<0.01). Observations demonstrated that in PCOS group, the Hcy level increased independent to E2, glucose levels, BMI and age, while the PCOS group showed significantly higher BMI compared with non-PCOS group (p=0.03). However, no significant differences were revealed between groups for FF glucose and E2 levels. Conclusion: Present data showed that although FF glucose and E2 levels were constant in PCOS and non PCOS patients, but the FF Hcy levels in PCOS were significantly increased (p=0.01). PMID:27679823

  9. Circulating TRAIL shows a significant post-partum decline associated to stressful conditions.

    Directory of Open Access Journals (Sweden)

    Giorgio Zauli

    Full Text Available BACKGROUND: Since circulating levels of TNF-related apoptosis inducing ligand (TRAIL may be important in the physiopathology of pregnancy, we tested the hypothesis that TRAIL levels change at delivery in response to stressful conditions. METHODS/PRINCIPAL FINDINGS: We conducted a longitudinal study in a cohort of 73 women examined at week 12, week 16, delivery and in the corresponding cord blood (CB. Serum TRAIL was assessed in relationship with maternal characteristics and to biochemical parameters. TRAIL did not vary between 12 (67.6±27.6 pg/ml, means±SD and 16 (64.0±16.2 pg/ml weeks' gestation, while displaying a significant decline after partum (49.3±26.4 pg/ml. Using a cut-off decline >20 pg/ml between week 12 and delivery, the subset of women with the higher decline of circulating TRAIL (41.7% showed the following characteristics: i nullipara, ii higher age, iii operational vaginal delivery or urgent CS, iv did not receive analgesia during labor, v induced labor. CB TRAIL was significantly higher (131.6±52 pg/ml with respect to the corresponding maternal TRAIL, and the variables significantly associated with the first quartile of CB TRAIL (<90 pg/ml were higher pre-pregnancy BMI, induction of labor and fetal distress. With respect to the biochemical parameters, maternal TRAIL at delivery showed an inverse correlation with C-reactive protein (CRP, total cortisol, glycemia and insulin at bivariate analysis, but only with CRP at multivariate analysis. CONCLUSIONS: Stressful partum conditions and elevated CRP levels are associated with a decrease of circulating TRAIL.

  10. Impact Factors Show Increased Use of AGU Journals in 2008

    Science.gov (United States)

    Ford, Barbara Meyers

    2009-07-01

    The latest numbers released from Journal Citation Reports (JCR), published annually by Thomson Reuters, show large increases in the impact factor (IF) for several AGU journals. IFs are one way for publishers to know that readers have found their journals useful and of value in research. A journal's IF is calculated by taking the total number of citations to articles published by a given journal in the past 2 years and dividing it by the total number of papers published by the journal in the same time period. More generally, it can be seen as the frequency with which articles in a journal have been cited over the past year. The numbers speak for themselves (see Table 1).

  11. FES Training in Aging: interim results show statistically significant improvements in mobility and muscle fiber size

    Directory of Open Access Journals (Sweden)

    Helmut Kern

    2012-03-01

    Full Text Available Aging is a multifactorial process that is characterized by decline in muscle mass and performance. Several factors, including reduced exercise, poor nutrition and modified hormonal metabolism, are responsible for changes in the rates of protein synthesis and degradation that drive skeletal muscle mass reduction with a consequent decline of force generation and mobility functional performances. Seniors with normal life style were enrolled: two groups in Vienna (n=32 and two groups in Bratislava: (n=19. All subjects were healthy and declared not to have any specific physical/disease problems. The two Vienna groups of seniors exercised for 10 weeks with two different types of training (leg press at the hospital or home-based functional electrical stimulation, h-b FES. Demografic data (age, height and weight were recorded before and after the training period and before and after the training period the patients were submitted to mobility functional analyses and muscle biopsies. The mobility functional analyses were: 1. gait speed (10m test fastest speed, in m/s; 2. time which the subject needed to rise from a chair for five times (5x Chair-Rise, in s; 3. Timed –Up-Go- Test, in s; 4. Stair-Test, in s; 5. isometric measurement of quadriceps force (Torque/kg, in Nm/kg; and 6. Dynamic Balance in mm. Preliminary analyses of muscle biopsies from quadriceps in some of the Vienna and Bratislava patients present morphometric results consistent with their functional behaviors. The statistically significant improvements in functional testings here reported demonstrates the effectiveness of h-b FES, and strongly support h-b FES, as a safe home-based method to improve contractility and performances of ageing muscles.

  12. Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux.

    Directory of Open Access Journals (Sweden)

    Nicola K Petty

    2011-04-01

    Full Text Available Citrobacter rodentium is a natural mouse pathogen that causes attaching and effacing (A/E lesions. It shares a common virulence strategy with the clinically significant human A/E pathogens enteropathogenic E. coli (EPEC and enterohaemorrhagic E. coli (EHEC and is widely used to model this route of pathogenesis. We previously reported the complete genome sequence of C. rodentium ICC168, where we found that the genome displayed many characteristics of a newly evolved pathogen. In this study, through PFGE, sequencing of isolates showing variation, whole genome transcriptome analysis and examination of the mobile genetic elements, we found that, consistent with our previous hypothesis, the genome of C. rodentium is unstable as a result of repeat-mediated, large-scale genome recombination and because of active transposition of mobile genetic elements such as the prophages. We sequenced an additional C. rodentium strain, EX-33, to reveal that the reference strain ICC168 is representative of the species and that most of the inactivating mutations were common to both isolates and likely to have occurred early on in the evolution of this pathogen. We draw parallels with the evolution of other bacterial pathogens and conclude that C. rodentium is a recently evolved pathogen that may have emerged alongside the development of inbred mice as a model for human disease.

  13. The genome-scale metabolic extreme pathway structure in Haemophilus influenzae shows significant network redundancy.

    Science.gov (United States)

    Papin, Jason A; Price, Nathan D; Edwards, Jeremy S; Palsson B, Bernhard Ø

    2002-03-07

    Genome-scale metabolic networks can be characterized by a set of systemically independent and unique extreme pathways. These extreme pathways span a convex, high-dimensional space that circumscribes all potential steady-state flux distributions achievable by the defined metabolic network. Genome-scale extreme pathways associated with the production of non-essential amino acids in Haemophilus influenzae were computed. They offer valuable insight into the functioning of its metabolic network. Three key results were obtained. First, there were multiple internal flux maps corresponding to externally indistinguishable states. It was shown that there was an average of 37 internal states per unique exchange flux vector in H. influenzae when the network was used to produce a single amino acid while allowing carbon dioxide and acetate as carbon sinks. With the inclusion of succinate as an additional output, this ratio increased to 52, a 40% increase. Second, an analysis of the carbon fates illustrated that the extreme pathways were non-uniformly distributed across the carbon fate spectrum. In the detailed case study, 45% of the distinct carbon fate values associated with lysine production represented 85% of the extreme pathways. Third, this distribution fell between distinct systemic constraints. For lysine production, the carbon fate values that represented 85% of the pathways described above corresponded to only 2 distinct ratios of 1:1 and 4:1 between carbon dioxide and acetate. The present study analysed single outputs from one organism, and provides a start to genome-scale extreme pathways studies. These emergent system-level characterizations show the significance of metabolic extreme pathway analysis at the genome-scale.

  14. Music programs designed to remedy burnout symptoms show significant effects after five weeks.

    Science.gov (United States)

    Brandes, Vera; Terris, Darcey D; Fischer, Claudia; Schuessler, Marc N; Ottowitz, Gernot; Titscher, Georg; Fischer, Joachim E; Thayer, Julian F

    2009-07-01

    Earlier studies have demonstrated that music interventions can lessen symptoms of depression. Depression and burnout are closely related. We hypothesized that specially designed receptive music therapy programs and protocols might reduce the symptoms of burnout. In a four-arm randomized, placebo- and waiting-list-controlled double-blind study, including 150 participants, two specific music programs significantly reduced burnout symptoms after 5 weeks. The effects were maintained over a long time period. This newly developed method of receptive music therapy was also evaluated for the treatment of depression and dysthymia, with significant outcomes.

  15. Fungal communities in wheat grain show significant co-existence patterns among species

    DEFF Research Database (Denmark)

    Nicolaisen, M.; Justesen, A. F.; Knorr, K.

    2014-01-01

    90 samples that were taken from locations across Denmark during three years. These sequences could be grouped into 173 non-singleton operational taxonomic units (OTUs) of which 21 OTUs, identified as belonging to genera such as Fusarium, Alternaria, Cladosporium. Phaeosphaeria and Microdochium, were...... the 21 core OTUs into three clusters: cluster 1 consisting of saprotrophs, cluster 2 consisting mainly of yeasts and saprotrophs and cluster 3 consisting of wheat pathogens. Principal component extraction showed that the Fusarium graminearum group was inversely related to OTUs of clusters 1 and 2....

  16. Strong Showing for AGU Journals in 2009 Impact Factors

    Science.gov (United States)

    Cook, Bill

    2010-06-01

    AGU publishes great science, which is recognized in several ways. One of the most widely recognized is from Thomson Reuters, which provides the Journal Citation Report (JCR) each year as a component of the Web of Science®. JCR reports on several measures of journal usage, including a journal's Eigenfactor score, its Article Influence score, its Impact Factor, and its rank within a cohort of similar journals. According to the 2009 statistics released last week, AGU again has outperformed its larger competitors. For the twelfth time, two different AGU titles hold the top rank in their categories, and AGU titles hold the second spot in two other categories and third in two more.

  17. The genus Drosophila is characterized by a large number of sibling species showing evolutionary significance

    Indian Academy of Sciences (India)

    BASHISTH N. SINGH

    2016-12-01

    Mayr (1942) defined sibling species as sympatric forms which are morphologically very similar or indistinguishable, but which possess specific biological characteristics and are reproductively isolated. Another term, cryptic species has also been used for such species. However, this concept changed later. Sibling species are as similar as twins. This category does not necessarily include phylogenetic siblings as members of a superspecies. Since the term sibling species was defined by Mayr, a large number of cases of sibling species pairs/groups have been reported and thus they are widespread in the animal kingdom.However, they seem to be more common in some groups such as insects. In insects, they have been reported in diptera, lepidoptera, coleoptera, orthoptera, hymenoptera and others. Sibling species are widespread among the dipteran insects and as such are well studied because some species are important medically (mosquitoes), genetically (Drosophila) and cytologically(Sciara and Chironomus). The well-studied classical pairs of sibling species in Drosophila are: D. pseudoobscura and D. persimilis, and D. melanogaster and D. simulans. Subsequently, a number of sibling species have been added to these pairs and a large number of other sibling species pairs/groups in different species groups of the genus Drosophila have been reported in literature. The present review briefly summarizes the cases of sibling species pairs/groups in the genus Drosophila with their evolutionary significance.

  18. Polyphenols from blossoms of Citrus aurantium L. var. amara Engl. show significant anti-complement and anti-inflammatory effects.

    Science.gov (United States)

    Shen, Chun-Yan; Jiang, Jian-Guo; Huang, Chun-Ling; Zhu, Wei; Zheng, Chao-Yang

    2017-09-24

    Citrus aurantium L. var. amara Engl. (CAVA) was traditionally used as a digestant or expectorant in China. Crude polyphenols (CAVAP-W) extracted from blossoms of CAVA were mainly composed of eriocitrin/neoeriocitrin, eriocitrin/neoeriocitrin, rhoifolin, hesperidin, naringin, rutin, veronicastroside, neohesperidin and hesperetin by LC-MS analysis. CAVAP-W showed significant anti-complement and anti-inflammatory effects. Due to the close relationship between anti-complement and anti-inflammatory activity, the anti-inflammatory effect was further investigated and the results showed that CAVAP-W significantly suppressed production of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and mRNA expression of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, IL-1β and cyclooxygenase-2 (COX-2) in lipopolysaccharides-stimulated RAW264.7 cells. Furthermore, CAVAP-W inhibited mitogen-activated protein kinase (MAPK) phosphorylation and NF-κB activation through suppressing nuclear translocation of nuclear factor-kappa B (NF-κB) P65, degradation and phosphorylation of IκBα, phosphorylation of IκKBɑ/ß, c-Jun N-terminal kinase (JNK) and P38, and activation of COX-2, thereby exerting the anti-inflammatory effects.

  19. Significant Factors Determining E-government Adoption in Selangor, Malaysia

    Directory of Open Access Journals (Sweden)

    Siti Hajar Mohd Idris

    2016-06-01

    Full Text Available Studies have shown that low adoption rate among citizens has been hindering the optimization of e-Government services especially in developing countries. Hence, one of the critical measures that has to be undertaken is to identify and overcome possible barriers to further facilitate a higher rate of adoption. A multistage stratified sampling was used in this study to collect data from 1000 respondents, both user and non-user residing in the state of Selangor, Malaysia. This state was chosen as to provide a better understanding of low adoption when issues of basic facilities have been successfully overcome. An exploratory factor analysis was performed to identify latent constructs and seven key factors were identified. A multiple regression model was subsequently used to analyze significant factors in determining the willingness to use e-Government services. The determinants are language barrier, educational level, secure, format, easy to use, enjoyable, reliable, visual appeal and infrastructure. The result shows significant variables that act as barriers to adoption are reliable, enjoyable, easy to use, secure, and language used. The constraints pointed out in the open ended questions mainly focus on the issue of accessibility, ease of use and awareness. Overcoming these obstacles is therefore crucial in order to enhance the usage of e-Government services which consequently will improve the quality of public administration in Malaysia.

  20. MICROENTERPRISES AND SIGNIFICANT RISK FACTORS IN LOAN PROCESS

    National Research Council Canada - National Science Library

    Jaroslav Belás; Sergej Vojtovic; Aleksandr Kljucnikov

    2016-01-01

    .... The aim of this paper was to define and quantify significant risk factors of microenterprises' credit financing in the current dynamic economic environment and to compare the significant position...

  1. Depressive symptoms and pain evaluations among persons with chronic pain: catastrophizing, but not pain acceptance, shows significant effects.

    Science.gov (United States)

    Richardson, Elizabeth J; Ness, Timothy J; Doleys, Daniel M; Baños, James H; Cianfrini, Leanne; Richards, J Scott

    2009-12-15

    Cognitive factors such as catastrophic thoughts regarding pain, and conversely, one's acceptance of that pain, may affect emotional functioning among persons with chronic pain conditions. The aims of the present study were to examine the effects of both catastrophizing and acceptance on affective ratings of experimentally induced ischemic pain and also self-reports of depressive symptoms. Sixty-seven individuals with chronic back pain completed self-report measures of catastrophizing, acceptance, and depressive symptoms. In addition, participants underwent an ischemic pain induction procedure and were asked to rate the induced pain. Catastrophizing showed significant effects on sensory and intensity but not affective ratings of the induced pain. Acceptance did not show any significant associations, when catastrophizing was also in the model, with any form of ratings of the induced pain. Catastrophizing, but not acceptance, was also significantly associated with self-reported depressive symptoms when these two variables were both included in a regression model. Overall, results indicate negative thought patterns such as catastrophizing appear to be more closely related to outcomes of perceived pain severity and affect in persons with chronic pain exposed to an experimental laboratory pain stimulus than does more positive patterns as reflected in measures of acceptance.

  2. Prognostic significance of selected lifestyle factors in urinary bladder cancer.

    Science.gov (United States)

    Wakai, K; Ohno, Y; Obata, K; Aoki, K

    1993-12-01

    To examine the prognostic significance of lifestyle factors in urinary bladder cancer, we conducted a follow-up study of 258 incident bladder cancer patients, who were originally recruited in a case-control study in metropolitan Nagoya. Information on individual survivals was obtained from the computer data-file of the tumor registry of the Nagoya Bladder Cancer Research Group. Univariate analyses revealed significant associations of 5-year survivorship with educational attainment, marital status, drinking habits and consumption of green tea in males, and age at first consultation, histological type and grade of tumor, stage and distant metastasis in both sexes. After adjustment for age, stage, histology (histological type and grade) and distant metastasis by means of a proportional hazards model, drinking of alcoholic beverages was significantly associated with the prognosis of bladder cancer in males. Its adjusted hazard ratio was 0.46 (95% confidence interval: 0.26-0.79), favoring patients who had taken alcoholic beverages. In detailed analysis, ex-drinkers and all levels of current drinkers demonstrated hazard ratios smaller than unity, although no clear dose-response relationship was detected. No prognostic significance was found for such lifestyle factors as smoking habit, uses of artificial sweeteners and hairdye, and consumption of coffee, black tea, matcha (powdered green tea) and cola.

  3. Functionally significant, rare transcription factor variants in tetralogy of Fallot.

    Directory of Open Access Journals (Sweden)

    Ana Töpf

    Full Text Available Rare variants in certain transcription factors involved in cardiac development cause Mendelian forms of congenital heart disease. The purpose of this study was to systematically assess the frequency of rare transcription factor variants in sporadic patients with the cardiac outflow tract malformation tetralogy of Fallot (TOF.We sequenced the coding, 5'UTR, and 3'UTR regions of twelve transcription factor genes implicated in cardiac outflow tract development (NKX2.5, GATA4, ISL1, TBX20, MEF2C, BOP/SMYD1, HAND2, FOXC1, FOXC2, FOXH, FOXA2 and TBX1 in 93 non-syndromic, non-Mendelian TOF cases. We also analysed Illumina Human 660W-Quad SNP Array data for copy number variants in these genes; none were detected. Four of the rare variants detected have previously been shown to affect transactivation in in vitro reporter assays: FOXC1 p.P297S, FOXC2 p.Q444R, FOXH1 p.S113T and TBX1 p.P43_G61del PPPPRYDPCAAAAPGAPGP. Two further rare variants, HAND2 p.A25_A26insAA and FOXC1 p.G378_G380delGGG, A488_491delAAAA, affected transactivation in in vitro reporter assays. Each of these six functionally significant variants was present in a single patient in the heterozygous state; each of the four for which parental samples were available were maternally inherited. Thus in the 93 TOF cases we identified six functionally significant mutations in the secondary heart field transcriptional network.This study indicates that rare genetic variants in the secondary heart field transcriptional network with functional effects on protein function occur in 3-13% of patients with TOF. This is the first report of a functionally significant HAND2 mutation in a patient with congenital heart disease.

  4. Functionally significant, rare transcription factor variants in tetralogy of Fallot.

    Science.gov (United States)

    Töpf, Ana; Griffin, Helen R; Glen, Elise; Soemedi, Rachel; Brown, Danielle L; Hall, Darroch; Rahman, Thahira J; Eloranta, Jyrki J; Jüngst, Christoph; Stuart, A Graham; O'Sullivan, John; Keavney, Bernard D; Goodship, Judith A

    2014-01-01

    Rare variants in certain transcription factors involved in cardiac development cause Mendelian forms of congenital heart disease. The purpose of this study was to systematically assess the frequency of rare transcription factor variants in sporadic patients with the cardiac outflow tract malformation tetralogy of Fallot (TOF). We sequenced the coding, 5'UTR, and 3'UTR regions of twelve transcription factor genes implicated in cardiac outflow tract development (NKX2.5, GATA4, ISL1, TBX20, MEF2C, BOP/SMYD1, HAND2, FOXC1, FOXC2, FOXH, FOXA2 and TBX1) in 93 non-syndromic, non-Mendelian TOF cases. We also analysed Illumina Human 660W-Quad SNP Array data for copy number variants in these genes; none were detected. Four of the rare variants detected have previously been shown to affect transactivation in in vitro reporter assays: FOXC1 p.P297S, FOXC2 p.Q444R, FOXH1 p.S113T and TBX1 p.P43_G61del PPPPRYDPCAAAAPGAPGP. Two further rare variants, HAND2 p.A25_A26insAA and FOXC1 p.G378_G380delGGG, A488_491delAAAA, affected transactivation in in vitro reporter assays. Each of these six functionally significant variants was present in a single patient in the heterozygous state; each of the four for which parental samples were available were maternally inherited. Thus in the 93 TOF cases we identified six functionally significant mutations in the secondary heart field transcriptional network. This study indicates that rare genetic variants in the secondary heart field transcriptional network with functional effects on protein function occur in 3-13% of patients with TOF. This is the first report of a functionally significant HAND2 mutation in a patient with congenital heart disease.

  5. Analysis of significant factors for dengue fever incidence prediction.

    Science.gov (United States)

    Siriyasatien, Padet; Phumee, Atchara; Ongruk, Phatsavee; Jampachaisri, Katechan; Kesorn, Kraisak

    2016-04-16

    Many popular dengue forecasting techniques have been used by several researchers to extrapolate dengue incidence rates, including the K-H model, support vector machines (SVM), and artificial neural networks (ANN). The time series analysis methodology, particularly ARIMA and SARIMA, has been increasingly applied to the field of epidemiological research for dengue fever, dengue hemorrhagic fever, and other infectious diseases. The main drawback of these methods is that they do not consider other variables that are associated with the dependent variable. Additionally, new factors correlated to the disease are needed to enhance the prediction accuracy of the model when it is applied to areas of similar climates, where weather factors such as temperature, total rainfall, and humidity are not substantially different. Such drawbacks may consequently lower the predictive power for the outbreak. The predictive power of the forecasting model-assessed by Akaike's information criterion (AIC), Bayesian information criterion (BIC), and the mean absolute percentage error (MAPE)-is improved by including the new parameters for dengue outbreak prediction. This study's selected model outperforms all three other competing models with the lowest AIC, the lowest BIC, and a small MAPE value. The exclusive use of climate factors from similar locations decreases a model's prediction power. The multivariate Poisson regression, however, effectively forecasts even when climate variables are slightly different. Female mosquitoes and seasons were strongly correlated with dengue cases. Therefore, the dengue incidence trends provided by this model will assist the optimization of dengue prevention. The present work demonstrates the important roles of female mosquito infection rates from the previous season and climate factors (represented as seasons) in dengue outbreaks. Incorporating these two factors in the model significantly improves the predictive power of dengue hemorrhagic fever forecasting

  6. Pomalidomide shows significant therapeutic activity against CNS lymphoma with a major impact on the tumor microenvironment in murine models.

    Science.gov (United States)

    Li, Zhimin; Qiu, Yushi; Personett, David; Huang, Peng; Edenfield, Brandy; Katz, Jason; Babusis, Darius; Tang, Yang; Shirely, Michael A; Moghaddam, Mehran F; Copland, John A; Tun, Han W

    2013-01-01

    Primary CNS lymphoma carries a poor prognosis. Novel therapeutic agents are urgently needed. Pomalidomide (POM) is a novel immunomodulatory drug with anti-lymphoma activity. CNS pharmacokinetic analysis was performed in rats to assess the CNS penetration of POM. Preclinical evaluation of POM was performed in two murine models to assess its therapeutic activity against CNS lymphoma. The impact of POM on the CNS lymphoma immune microenvironment was evaluated by immunohistochemistry and immunofluorescence. In vitro cell culture experiments were carried out to further investigate the impact of POM on the biology of macrophages. POM crosses the blood brain barrier with CNS penetration of ~ 39%. Preclinical evaluations showed that it had significant therapeutic activity against CNS lymphoma with significant reduction in tumor growth rate and prolongation of survival, that it had a major impact on the tumor microenvironment with an increase in macrophages and natural killer cells, and that it decreased M2-polarized tumor-associated macrophages and increased M1-polarized macrophages when macrophages were evaluated based on polarization status. In vitro studies using various macrophage models showed that POM converted the polarization status of IL4-stimulated macrophages from M2 to M1, that M2 to M1 conversion by POM in the polarization status of lymphoma-associated macrophages is dependent on the presence of NK cells, that POM induced M2 to M1 conversion in the polarization of macrophages by inactivating STAT6 signaling and activating STAT1 signaling, and that POM functionally increased the phagocytic activity of macrophages. Based on our findings, POM is a promising therapeutic agent for CNS lymphoma with excellent CNS penetration, significant preclinical therapeutic activity, and a major impact on the tumor microenvironment. It can induce significant biological changes in tumor-associated macrophages, which likely play a major role in its therapeutic activity against CNS

  7. Assessment of postmenopausal women and significant risk factors for osteoporosis.

    Science.gov (United States)

    Schnatz, Peter F; Marakovits, Kimberly A; O'Sullivan, David M

    2010-09-01

    The assessment of osteoporosis risk factors can help guide early intervention. The objective of this study was to analyze numerous potential risk factors to see which were associated with postmenopausal osteoporosis. Women aged 49 or greater presenting for dual-energy x-ray absorptiometry bone scans were recruited from radiology sites in the Hartford, Connecticut, area between January 2007 and March 2009, inclusive. Information was collected regarding primary and secondary risk factors for osteoporosis development, as well as family history and history of pregnancy and breast-feeding. Survey results were subsequently correlated with each woman's dual-energy x-ray absorptiometry scan results. In a sample of 619 women, history of fracture (odds ratio [OR], 12.49), weight less than 127 pounds (OR, 3.50), and use of anticoagulants (OR, 5.40) increased the chance of developing osteoporosis. In contrast, multiparity (OR, 0.45) and history of breast-feeding (OR, 0.38) decreased the development of osteoporosis in postmenopausal women. In women aged 49 to 54, breast-feeding was significantly protective, while low body mass index was most indicative of osteoporosis in women ages 55 to 64. Both previous fracture and low body mass index were associated with osteoporosis in women over age 64. The current results are consistent with other studies suggesting that previous fracture, low body weight, and use of anticoagulants increase the risk of osteoporosis. Our results also suggest that a history of pregnancy and breast-feeding protects against the development of postmenopausal osteoporosis, especially in women aged 49 to 54.

  8. Dimethyl fumarate-associated lymphopenia: Risk factors and clinical significance

    Science.gov (United States)

    Longbrake, Erin E; Naismith, Robert T; Parks, Becky J; Wu, Gregory F; Cross, Anne H

    2015-01-01

    Background Dimethyl fumarate (DMF), a disease-modifying therapy for multiple sclerosis (MS), causes lymphopenia in a fraction of patients. The clinical significance of this is unknown. Several cases of progressive multifocal leukoencephalopathy in lymphopenic fumarate-treated patients have raised concerns about drug safety. Since lymphocytes contribute to MS pathology, lymphopenia may also be a biomarker for response to the drug. Objective The objective of this manuscript is to evaluate risk factors for DMF-induced lymphopenia and drug failure in a real-world population of MS patients. Methods We conducted a retrospective cohort study of 221 patients prescribed DMF at a single academic medical center between March 2013 and February 2015. Results Grade 2–3 lymphopenia developed in 17% of the total cohort and did not resolve during DMF treatment. Older age (>55), lower baseline absolute lymphocyte count and recent natalizumab exposure increased the risk of developing moderate to severe lymphopenia while on DMF. Lymphopenia was not predictive of good clinical response or of breakthrough MS activity on DMF. Conclusions Lymphopenia develops in a significant minority of DMF-treated patients, and if grade 2 or worse, is unlikely to resolve while on the drug. Increased vigilance in lymphocyte monitoring and infection awareness is particularly warranted in older patients and those switching from natalizumab. PMID:26550483

  9. Is pain a significant factor in elder self-neglect?

    Science.gov (United States)

    Pickens, Sabrina; Burnett, Jason; Naik, Aanand D; Holmes, Holly M; Dyer, Carmel Bitondo

    2006-01-01

    Pain is a worldwide health concern leading to cognitive impairments, depression, and decline in activities of daily living when poorly controlled. Self-neglect is also a serious public health issue being the most common allegation reported to Adult Protective Services (APS). The purpose of this analysis is to determine whether self-reported pain is associated with validated cases of self-neglect compared with matched controls. This is a cross-sectional study of 80 (APS) validated cases of self-neglect aged 65 years and older and 80 control participants recruited from a geriatric clinic at Harris County Hospital District. Control participants were matched for age, race, gender, and zip code. Both groups were administered the Wong-Baker FACES rating scale and the Mini-Mental State Examination in their homes by a geriatric nurse practitioner and a research assistant. Self-reported pain was noted in 43% of the self-neglect group compared with only 28% in the control group (X2 = 3.85, df = 1, p = .05). This difference became non-significant after stratifying for MMSE scores of 19 or greater (X2 = 3.38, df = 1, p = .066). The self-neglecting elderly also reported significantly higher levels of pain compared with the matched controls (t = 2.5, df = 143, p = .014). This mean difference remained significant after stratifying by MMSE scores of 19 or greater (t = 2.6, df = 124, p = .009). The data provide preliminary support for the relationship between self-reported pain and elder self-neglect. Research is needed to determine whether pain is a causal or associated factor in self-neglect and whether therapeutic intervention can improve the syndrome of self-neglect.

  10. Immunosuppression using the mammalian target of rapamycin (mTOR) inhibitor everolimus: pilot study shows significant cognitive and affective improvement.

    Science.gov (United States)

    Lang, U E; Heger, J; Willbring, M; Domula, M; Matschke, K; Tugtekin, S M

    2009-12-01

    Immunosuppression using calcineurin inhibitors (CNIs) is accompanied by neuropsychiatric side effects, which counteract longevity and quality of life benefits in 10% to 28% of patients. Following the availability of the mammalian target of rapamycin (mTOR) inhibitors, it became possible to replace CNI without increasing the risk of acute graft rejection. mTOR, a member of the phosphatidyl inositol 3' kinase family, is a downstream target of brain-derived neurotrophic factor, which has been implicated in the pathophysiology and treatment of several psychiatric disorders. Preclinical evidence has implicated the mTOR pathway in synaptic plasticity and fear memory consolidation and reconsolidation. In the present study we prospectively evaluated the psychiatric outcomes of CNI-free immunosuppression in adult maintenance heart transplant recipients (n = 9; age: 66.1 +/- 6.1) using the Wechsler Memory Scale-Revised (WMS-R), Symptom Checklist-90-Revised (SCL-90-R), Beck Depression Inventory (BDI), Trail Making Tests A and B, Digit Span (DS), and Hamilton Depression Scale (HAMD). Four weeks after switching to CNI-free immunosuppression using everolimus, BDI (Z = -1.14; P = .048), Trail Making tests A and B (Z = -2.52; P = .012), WMS-R (Z = 2.37; P = .018), and SCL-90-R (Z = -2.37; P = .018) were all significantly improved while DS (Z = -1.18; P = .236) and HAMD (Z = -0.595; P = .552) remained unchanged. This report describes favorable psychiatric outcome variables using everolimus in maintenance heart transplant recipients. CNI-free immunosuppression with everolimus might provide significant improvement in memory, concentration, and overall psychiatric symptoms among heart transplant recipients.

  11. CD38 Knockout Mice Show Significant Protection Against Ischemic Brain Damage Despite High Level Poly-ADP-Ribosylation.

    Science.gov (United States)

    Long, Aaron; Park, Ji H; Klimova, Nina; Fowler, Carol; Loane, David J; Kristian, Tibor

    2017-01-01

    Several enzymes in cellular bioenergetics metabolism require NAD(+) as an essential cofactor for their activity. NAD(+) depletion following ischemic insult can result in cell death and has been associated with over-activation of poly-ADP-ribose polymerase PARP1 as well as an increase in NAD(+) consuming enzyme CD38. CD38 is an NAD(+) glycohydrolase that plays an important role in inflammatory responses. To determine the contribution of CD38 activity to the mechanisms of post-ischemic brain damage we subjected CD38 knockout (CD38KO) mice and wild-type (WT) mice to transient forebrain ischemia. The CD38KO mice showed a significant amelioration in both histological and neurologic outcome following ischemic insult. Decrease of hippocampal NAD(+) levels detected during reperfusion in WT mice was only transient in CD38KO animals, suggesting that CD38 contributes to post-ischemic NAD(+) catabolism. Surprisingly, pre-ischemic poly-ADP-ribose (PAR) levels were dramatically higher in CD38KO animals compared to WT animals and exhibited reduction post-ischemia in contrast to the increased levels in WT animals. The high PAR levels in CD38 mice were due to reduced expression levels of poly-ADP-ribose glycohydrolase (PARG). Thus, the absence of CD38 activity can not only directly affect inflammatory response, but also result in unpredicted alterations in the expression levels of enzymes participating in NAD(+) metabolism. Although the CD38KO mice showed significant protection against ischemic brain injury, the changes in enzyme activity related to NAD(+) metabolism makes the determination of the role of CD38 in mechanisms of ischemic brain damage more complex.

  12. Mefenamic acid in combination with ribavirin shows significant effects in reducing chikungunya virus infection in vitro and in vivo.

    Science.gov (United States)

    Rothan, Hussin A; Bahrani, Hirbod; Abdulrahman, Ammar Y; Mohamed, Zulqarnain; Teoh, Teow Chong; Othman, Shatrah; Rashid, Nurshamimi Nor; Rahman, Noorsaadah A; Yusof, Rohana

    2016-03-01

    Chikungunya virus (CHIKV) infection is a persistent problem worldwide due to efficient adaptation of the viral vectors, Aedes aegypti and Aedes albopictus mosquitoes. Therefore, the absence of effective anti-CHIKV drugs to combat chikungunya outbreaks often leads to a significant impact on public health care. In this study, we investigated the antiviral activity of drugs that are used to alleviate infection symptoms, namely, the non-steroidal anti-inflammatory drugs (NSAIDs), on the premise that active compounds with potential antiviral and anti-inflammatory activities could be directly subjected for human use to treat CHIKV infections. Amongst the various NSAID compounds, Mefenamic acid (MEFE) and Meclofenamic acid (MECLO) showed considerable antiviral activity against viral replication individually or in combination with the common antiviral drug, Ribavirin (RIBA). The 50% effective concentration (EC50) was estimated to be 13 μM for MEFE, 18 μM for MECLO and 10 μM for RIBA, while MEFE + RIBA (1:1) exhibited an EC50 of 3 μM, and MECLO + RIBA (1:1) was 5 μM. Because MEFE is commercially available and its synthesis is easier compared with MECLO, MEFE was selected for further in vivo antiviral activity analysis. Treatment with MEFE + RIBA resulted in a significant reduction of hypertrophic effects by CHIKV on the mouse liver and spleen. Viral titre quantification in the blood of CHIKV-infected mice through the plaque formation assay revealed that treatment with MEFE + RIBA exhibited a 6.5-fold reduction compared with untreated controls. In conclusion, our study demonstrated that MEFE in combination with RIBA exhibited significant anti-CHIKV activity by impairing viral replication in vitro and in vivo. Indeed, this finding may lead to an even broader application of these combinatorial treatments against other viral infections. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Analysis of a large dataset of mycorrhiza inoculation field trials on potato shows highly significant increases in yield.

    Science.gov (United States)

    Hijri, Mohamed

    2016-04-01

    An increasing human population requires more food production in nutrient-efficient systems in order to simultaneously meet global food needs while reducing the environmental footprint of agriculture. Arbuscular mycorrhizal fungi (AMF) have the potential to enhance crop yield, but their efficiency has yet to be demonstrated in large-scale crop production systems. This study reports an analysis of a dataset consisting of 231 field trials in which the same AMF inoculant (Rhizophagus irregularis DAOM 197198) was applied to potato over a 4-year period in North America and Europe under authentic field conditions. The inoculation was performed using a liquid suspension of AMF spores that was sprayed onto potato seed pieces, yielding a calculated 71 spores per seed piece. Statistical analysis showed a highly significant increase in marketable potato yield (ANOVA, P < 0.0001) for inoculated fields (42.2 tons/ha) compared with non-inoculated controls (38.3 tons/ha), irrespective of trial year. The average yield increase was 3.9 tons/ha, representing 9.5 % of total crop yield. Inoculation was profitable with a 0.67-tons/ha increase in yield, a threshold reached in almost 79 % of all trials. This finding clearly demonstrates the benefits of mycorrhizal-based inoculation on crop yield, using potato as a case study. Further improvements of these beneficial inoculants will help compensate for crop production deficits, both now and in the future.

  14. Microalbuminuria: It's Significance, risk factors and methods of ...

    African Journals Online (AJOL)

    Alasia Datonye

    factors such as obesity , smoking , low birthweight , male. 71 ... found in nondiabetic obese subjects . In obese subjects ... cause increase in the excretion of albumin in urine. Albumin ... color blocks-yellow, light brown, medium brown, brick red,.

  15. the significance of epidermal growth factor receptor and survivin ...

    African Journals Online (AJOL)

    2013-01-01

    Jan 1, 2013 ... SURVIVIN EXPRESSION IN BLADDER CANCER TISSUE AND URINE ... Objective: To assess whether epidermal growth factor receptor (EGFR) and survivin ..... lung cancer by the FDA in 2003 (28) and is currently.

  16. SIGNIFICANT FACTORS CAUSING COST OVERRUNS IN TELECOMMUNICATION PROJECTS IN NIGERIA

    Directory of Open Access Journals (Sweden)

    Oko John Ameh

    2010-12-01

    Full Text Available Information and Communication Technology (ICT provides enormous benefits to economic development. However, cost overruns are a worldwide phenomenon and pose a serious threat to the development of telecommunication infrastructure, which is the platform for ICT. It is imperative to examine the possible factors that could lead to cost overruns, in order to avert the associated catalytic effects on the development of other sectors of the economy. This study involves a questionnaire survey of 42 factors that were identified as having the potential to cause cost overruns in 53 telecommunication projects that are scattered over the six geopolitical zones in Nigeria. The results indicated that construction-related factors top the list of categories that cause cost overruns in telecommunication projects. The following factors were identified as major causes of cost overruns and are ranked in their order of importance: the lack of contractor experience on the telecommunication projects, the high cost of imported materials and the fluctuation in the prices of materials that are necessary for the telecommunication projects. The study recommends that contingency provisions should be put in place to mitigate these factors at the project conception stage.

  17. A large population histology study showing the lack of association between ALT elevation and significant fibrosis in chronic hepatitis B.

    Directory of Open Access Journals (Sweden)

    Wai-Kay Seto

    Full Text Available OBJECTIVE: We determined the association between various clinical parameters and significant liver injury in both hepatitis B e antigen (HBeAg-positive and HBeAg-negative patients. METHODS: From 1994 to 2008, liver biopsy was performed on 319 treatment-naïve CHB patients. Histologic assessment was based on the Knodell histologic activity index for necroinflammation and the Ishak fibrosis staging for fibrosis. RESULTS: 211 HBeAg-positive and 108 HBeAg-negative patients were recruited, with a median age of 31 and 46 years respectively. 9 out of 40 (22.5% HBeAg-positive patients with normal ALT had significant histologic abnormalities (necroinflammation grading ≥ 7 or fibrosis score ≥ 3. There was a significant difference in fibrosis scores among HBeAg-positive patients with an ALT level within the Prati criteria (30 U/L for men, 19 U/L for women and patients with a normal ALT but exceeding the Prati criteria (p = 0.024. Age, aspartate aminotransferase and platelet count were independent predictors of significant fibrosis in HBeAg-positive patients with an elevated ALT by multivariate analysis (p = 0.007, 0.047 and 0.045 respectively. HBV DNA and platelet count were predictors of significant fibrosis in HBeAg-negative disease (p = 0.020 and 0.015 respectively. An elevated ALT was not predictive of significant fibrosis for HBeAg-positive (p = 0.345 and -negative (p = 0.544 disease. There was no significant difference in fibrosis staging among ALT 1-2 × upper limit of normal (ULN and > × 2 ULN for both HBeAg-positive (p = 0.098 and -negative (p = 0.838 disease. CONCLUSION: An elevated ALT does not accurately predict significant liver injury. Decisions on commencing antiviral therapy should not be heavily based on a particular ALT threshold.

  18. 48 CFR 15.304 - Evaluation factors and significant subfactors.

    Science.gov (United States)

    2010-10-01

    ... decision; and (2) Support meaningful comparison and discrimination between and among competing proposals... requirements: (1) Price or cost to the Government shall be evaluated in every source selection (10 U.S.C. 2305... solicitation shall also state, at a minimum, whether all evaluation factors other than cost or price,...

  19. Retinal vascular calibres are significantly associated with cardiovascular risk factors

    DEFF Research Database (Denmark)

    von Hanno, T.; Bertelsen, G.; Sjølie, Anne K.

    2014-01-01

    ) and retinal venular calibre (central retinal vein equivalent) were measured computer-assisted on retinal photographs. Data on blood pressure, body mass index (BMI), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, glycosylated haemoglobin (HbA1c) and smoking were collected....... Association between retinal vessel calibre and the cardiovascular risk factors was assessed by multivariable linear and logistic regression analyses. Results: Retinal arteriolar calibre was independently associated with age, blood pressure, HbA1c and smoking in women and men, and with HDL cholesterol in men...... cardiovascular risk factors were independently associated with retinal vascular calibre, with stronger effect of HDL cholesterol and BMI in men than in women. Blood pressure and smoking contributed most to the explained variance....

  20. Obesity as a significant risk factor for endometrial cancer

    OpenAIRE

    Amer Suskic; Sanela Halilovic Suskic; Dejan Opric; Sinisa Maksimovic

    2016-01-01

    Background: Endometrial cancer is the most developed countries the most common invasive neoplasms of the female reproductive system. The most frequently mentioned risk factors, which are associated with the occurrence of endometrial cancer are obesity, infertility, high blood pressure, diabetes, liver disease, hormone-active tumors of the ovary, and the use of external estrogen. Methods: This paper deals with data of the patients treated for endometrial carcinoma in Public Hospitals in Tra...

  1. Significant association of insulin and proinsulin with clustering of cardiovascular risk factors

    Institute of Scientific and Technical Information of China (English)

    En-Zhi Jia; Xin-Li Li; Hai-Yan Wang; Wen-Zhu Ma; Zhi-Jian Yang; Shi-Wei Chen; Guang-Yao Qi; Chun-Fa You; Jian-Feng Ma; Jing-Xin Zhang; Zhen-Zhen Wang; Wei-Chong Qian

    2005-01-01

    AIM: To investigate the association between true insulin and proinsulin and clustering of cardiovascular risk factors.METHODS: Based on the random stratified sampling principles, 1196 Chinese people (533 males and 663 females,aged 35-59 years with an average age of 46.69 years) were recruited. Biotin-avidin based double monoclonal antibody ELISA method was used to detect the true insulin and proinsulin, and a risk factor score was set to evaluate individuals according to the number of risk factors.RESULTS: The median (quartile range) of true insulin and proinsulin was 4.91 mIu/L (3.01-7.09 mIu/L) and 3.49 pmol/L (2.14-5.68 pmol/L) respectively, and the true insulin level of female subjects was significantly higher than that of male subjects (P = 0.000), but the level of proinsulin displayed no significant difference between males and females (P = 0.566). The results of covariate ANOVA after age and sex were controlled showed that subjects with any of the risk factors had a significantly higher true insulin level (P = 0.002 for hypercholesterolemia, P = 0.021 for high low-density lipoprotein cholesterol, P = 0.003 for low high-density lipoprotein cholesterol, and P = 0.000 for other risk factors) and proinsulin level (P = 0.001 for low high-density lipoprotein cholesterol, and P = 0.000 for other risk factors)than those with no risk factors. Furthermore, subjects with higher risk factor scores had a higher true insulin and proinsulin level than those with lower risk factor scores (P = 0.000). The multiple linear regression models showed that true insulin and proinsulin were significantly related to cardiovascular risk factor scores respectively (P = 0.000).CONCLUSION: True insulin and proinsulin are significantly associated with the clustering of cardiovascular risk factors.

  2. Fourier transform infrared microspectroscopy shows significant differences between spectra of undifferentiated and polynucleated FLG 29.1 dried cells

    Science.gov (United States)

    Romano, Salvatore; Benvenuti, Susanna; Conti, Antonio; Benedetti, Enzo; Bramanti, Emilia; Rossi, Ilaria; Benedetti, Edoardo

    1994-02-01

    In a recent study made on cultures of human leukaemic cells (FLG 29.1 cell line) we were able to detect, by IR microspectroscopy, some significant IR spectroscopic variations following differentiation of cells towards osteoclastic-like behavior. The present study was undertaken on the same cell line in order to monitor biochemical structure variations following fusion induced by polyetilenglycole (PEG), using FTIR microspectroscopy. The finger-print region of all the spectra was retained and normalized according to a new regression procedure. Eleven bands were selected and total band power and mean power per unit frequency were compared with the corresponding reference session bands by a Dunnett's T test. Significant differences were found in both the tested variables only between treated and untreated cells, in 6 bands.

  3. FOXO Transcription Factors: Their Clinical Significance and Regulation

    Directory of Open Access Journals (Sweden)

    Yu Wang

    2014-01-01

    Full Text Available Members of the class O of forkhead box transcription factors (FOXO have important roles in metabolism, cellular proliferation, stress resistance, and apoptosis. The activity of FOXOs is tightly regulated by posttranslational modification, including phosphorylation, acetylation, and ubiquitylation. Activation of cell survival pathways such as phosphoinositide-3-kinase/AKT/IKK or RAS/mitogen-activated protein kinase phosphorylates FOXOs at different sites which regulate FOXOs nuclear localization or degradation. FOXO transcription factors are upregulated in a number of cell types including hepatocytes, fibroblasts, osteoblasts, keratinocytes, endothelial cells, pericytes, and cardiac myocytes. They are involved in a number of pathologic and physiologic processes that include proliferation, apoptosis, autophagy, metabolism, inflammation, cytokine expression, immunity, differentiation, and resistance to oxidative stress. These processes impact a number of clinical conditions such as carcinogenesis, diabetes, diabetic complications, cardiovascular disease, host response, and wound healing. In this paper, we focus on the potential role of FOXOs in different disease models and the regulation of FOXOs by various stimuli.

  4. Rethinking the meaning and significance of journal impact factors.

    Science.gov (United States)

    Ironside, Pamela M

    2007-06-01

    It is not the case that impact factors are unhelpful and that their use should be abolished. Indeed, ISI is continuing to refine existing and develop new databases to provide important information to researchers, administrators, librarians, and editors. Impact factor data do provide useful information for the review process if used judiciously and with an awareness of what these data do and do not indicate. Perhaps it is timely for members of the nursing discipline to think more broadly about the nature of impact and to talk more about the ways in which review processes can account for the many ways the impact of research can be demonstrated. For example, as part of the review process, reviewers might ask each researcher to provide exemplars from teaching or clinical practice settings in which their research is actually being used. Researchers might also be asked to describe the impact that has occurred in ways other than through publication (e.g., presentations, consulting) and how this impact was determined. Creating review processes that allow researchers to describe their decision making related to disseminating their research and how this reflects their ability to influence the discipline may provide a more realistic picture of impact than calculated figures alone.

  5. Even mild cases of paediatric Henoch-Schönlein purpura nephritis show significant long-term proteinuria.

    Science.gov (United States)

    Tudorache, Elena; Azema, Christine; Hogan, Julien; Wannous, Hala; Aoun, Bilal; Decramer, Stéphane; Deschênes, Georges; Ulinski, Tim

    2015-08-01

    Henoch-Schonlein purpura (HSP) is a common cause of paediatric renal disease in children, representing 10-15% of paediatric glomerulonephritis. This study examined the long-term outcome of biopsy-proven HSP nephritis to identify correlations between disease development and treatment. Patients from three French centres were retrospectively analysed. We followed up 142 patients aged from 2 to 10.5 years with HSP nephritis, graded according to the International Study Group of Kidney Disease in Childhood classification. Mean (±SD) age at presentation was 7.6 ± 2.8 years. Nephrotic range proteinuria was present in 28% of patients with Grade II lesions, 60% with Grade III and 90% with Grade IV. Significant proteinuria (>0.5 g/L) was found in nine of 48 patients 3 years after renal biopsy, eight of 25 patients after 5 years and three of 14 patients after 10 years. There was no correlation between the proteinuria risk at 3, 5 or 10 years and the initial histological lesion or treatment modality. Treatment with angiotensin-converting enzyme inhibitors/angiotensin receptor blockers (ACEi/ARB) was linked to lower proteinuria, especially if it was started precociously. Even mild forms of HSP nephritis risk significant long-term proteinuria. Very early introduction of ACEi/ARB may improve the long-term outcome independent of histological lesions. ©2014 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.

  6. Human and animal isolates of Yersinia enterocolitica show significant serotype-specific colonization and host-specific immune defense properties.

    Science.gov (United States)

    Schaake, Julia; Kronshage, Malte; Uliczka, Frank; Rohde, Manfred; Knuuti, Tobias; Strauch, Eckhard; Fruth, Angelika; Wos-Oxley, Melissa; Dersch, Petra

    2013-11-01

    Yersinia enterocolitica is a human pathogen that is ubiquitous in livestock, especially pigs. The bacteria are able to colonize the intestinal tract of a variety of mammalian hosts, but the severity of induced gut-associated diseases (yersiniosis) differs significantly between hosts. To gain more information about the individual virulence determinants that contribute to colonization and induction of immune responses in different hosts, we analyzed and compared the interactions of different human- and animal-derived isolates of serotypes O:3, O:5,27, O:8, and O:9 with murine, porcine, and human intestinal cells and macrophages. The examined strains exhibited significant serotype-specific cell binding and entry characteristics, but adhesion and uptake into different host cells were not host specific and were independent of the source of the isolate. In contrast, survival and replication within macrophages and the induced proinflammatory response differed between murine, porcine, and human macrophages, suggesting a host-specific immune response. In fact, similar levels of the proinflammatory cytokine macrophage inflammatory protein 2 (MIP-2) were secreted by murine bone marrow-derived macrophages with all tested isolates, but the equivalent interleukin-8 (IL-8) response of porcine bone marrow-derived macrophages was strongly serotype specific and considerably lower in O:3 than in O:8 strains. In addition, all tested Y. enterocolitica strains caused a considerably higher level of secretion of the anti-inflammatory cytokine IL-10 by porcine than by murine macrophages. This could contribute to limiting the severity of the infection (in particular of serotype O:3 strains) in pigs, which are the primary reservoir of Y. enterocolitica strains pathogenic to humans.

  7. Fusion of protegrin-1 and plectasin to MAP30 shows significant inhibition activity against dengue virus replication.

    Directory of Open Access Journals (Sweden)

    Hussin A Rothan

    Full Text Available Dengue virus (DENV broadly disseminates in tropical and sub-tropical countries and there are no vaccine or anti-dengue drugs available. DENV outbreaks cause serious economic burden due to infection complications that requires special medical care and hospitalization. This study presents a new strategy for inexpensive production of anti-DENV peptide-fusion protein to prevent and/or treat DENV infection. Antiviral cationic peptides protegrin-1 (PG1 and plectasin (PLSN were fused with MAP30 protein to produce recombinant antiviral peptide-fusion protein (PG1-MAP30-PLSN as inclusion bodies in E. coli. High yield production of PG1-MAP30-PLSN protein was achieved by solubilization of inclusion bodies in alkaline buffer followed by the application of appropriate refolding techniques. Antiviral PG1-MAP30-PLSN protein considerably inhibited DENV protease (NS2B-NS3pro with half-maximal inhibitory concentration (IC50 0.5±0.1 μM. The real-time proliferation assay (RTCA and the end-point proliferation assay (MTT assay showed that the maximal-nontoxic dose of the peptide-fusion protein against Vero cells is approximately 0.67±0.2 μM. The cell-based assays showed considerable inhibition of the peptide-fusion protein against binding and proliferating stages of DENV2 into the target cells. The peptide-fusion protein protected DENV2-challeged mice with 100% of survival at the dose of 50 mg/kg. In conclusion, producing recombinant antiviral peptide-fusion protein by combining short antiviral peptide with a central protein owning similar activity could be useful to minimize the overall cost of short peptide production and take advantage of its synergistic antiviral activities.

  8. Alcohol-related genes show an enrichment of associations with a persistent externalizing factor.

    Science.gov (United States)

    Ashenhurst, James R; Harden, K Paige; Corbin, William R; Fromme, Kim

    2016-10-01

    Research using twins has found that much of the variability in externalizing phenotypes-including alcohol and drug use, impulsive personality traits, risky sex, and property crime-is explained by genetic factors. Nevertheless, identification of specific genes and variants associated with these traits has proven to be difficult, likely because individual differences in externalizing are explained by many genes of small individual effect. Moreover, twin research indicates that heritable variance in externalizing behaviors is mostly shared across the externalizing spectrum rather than specific to any behavior. We use a longitudinal, "deep phenotyping" approach to model a general externalizing factor reflecting persistent engagement in a variety of socially problematic behaviors measured at 11 assessment occasions spanning early adulthood (ages 18 to 28). In an ancestrally homogenous sample of non-Hispanic Whites (N = 337), we then tested for enrichment of associations between the persistent externalizing factor and a set of 3,281 polymorphisms within 104 genes that were previously identified as associated with alcohol-use behaviors. Next, we tested for enrichment among domain-specific factors (e.g., property crime) composed of residual variance not accounted for by the common factor. Significance was determined relative to bootstrapped empirical thresholds derived from permutations of phenotypic data. Results indicated significant enrichment of genetic associations for persistent externalizing, but not for domain-specific factors. Consistent with twin research findings, these results suggest that genetic variants are broadly associated with externalizing behaviors rather than unique to specific behaviors. (PsycINFO Database Record

  9. Takotsubo Cardiomyopathy: A Long Term Follow-up Shows Benefit with Risk Factor Reduction

    Directory of Open Access Journals (Sweden)

    Koroush Khalighi

    2015-11-01

    Full Text Available Only sparse data was available on long-term of Takotusbo Cardiomyopathy (TC. Previous studies suggested prognosis is not necessarily benign. We report the long-term follow-up of 12 TC patients actively managed with risk factor reduction. Retrospective analysis of all patients diagnosed with TC at our hospital between 1998 and 2010. We identified 12 patients with TC among 1651 cases of emergent left heart catheterization over 12 years. Mean follow-up time was 8.3 ± 3.6 years. All were female, 87% had hypertension, 25% had history of Coronary Artery Disease (CAD, 67% had hyperlipidemia, 44% had some preceding emotional trauma, and 44% had some physical/physiological stress. Previous studies have shown that over 50% of TC patients experience future cardiac events, and 10% have a recurrence of TC. Patients were prescribed therapeutic lifestyle changes (TLC and guideline directed medical therapy (GDMT for aggressive risk factor reduction. TLC included diet, exercise, and cardiac rehabilitation. GDMT often included aspirin, beta-blockers, ACE-inhibitors, and statins. Follow-up echocardiograms showed recovery and maintenance of the ejection fraction. There was no cardiac mortality and no recurrences of TC. Aggressive risk factor reduction with TLC and GDMT may be effective in improving the long term outcomes of patients with TC.

  10. Mortality risk factors show similar trends in modern and historic populations exposed to plague.

    Science.gov (United States)

    Rubini, Mauro; Gualdi-Russo, Emanuela; Manzon, Vanessa S; Rinaldo, Natascia; Bianucci, Raffaella

    2016-05-31

    Plague has been responsible for two major historic pandemics (6th-8th century CE; 14th-19th century CE) and a modern one. The recent Malagasy plague outbreaks raised new concerns on the deadly potential of the plague-causing bacteria Yersinia pestis. Between September 2014 and April 2015, outbreaks of bubonic and pneumonic plague hit the Malagasy population. Two hundred and sixty-three cases, including 71 deaths, have been reported in 16 different districts with a case fatality rate of 27%. The scope of our study was to ascertain whether the risk factors for health in modern-day populations exposed to plague and in ancient populations that faced the two historic pandemics varied or remained substantially unaltered. The risk of mortality of the Malagasy population with those obtained from the reconstruction of three samples of European populations exposed to the historic pandemics was contrasted. The evidence shows that the risks of death are not uniform across age neither in modern nor in historic populations exposed to plague and shows precise concentrations in specific age groups (children between five and nine years of age and young adults). Although in the post-antibiotic era, the fatality rates have drastically reduced, both modern and historic populations were exposed to the same risk factors that are essentially represented by a low standard of environmental hygiene, poor nutrition, and weak health systems.

  11. Examining significant factors in micro and small enterprises performance: case study in Amhara region, Ethiopia

    Science.gov (United States)

    Cherkos, Tomas; Zegeye, Muluken; Tilahun, Shimelis; Avvari, Muralidhar

    2017-07-01

    Furniture manufacturing micro and small enterprises are confronted with several factors that affect their performance. Some enterprises fail to sustain, some others remain for long period of time without transforming, and most are producing similar and non-standard products. The main aim of this manuscript is on improving the performance and contribution of MSEs by analyzing impact of significant internal and external factors. Data was collected via a questionnaire, group discussion with experts and interviewing process. Randomly selected eight representative main cities of Amhara region with 120 furniture manufacturing enterprises are considered. Data analysis and presentation was made using SPSS tools (correlation, proximity, and T test) and impact-effort analysis matrix tool. The correlation analysis shows that politico-legal with infrastructure, leadership with entrepreneurship skills and finance and credit with marketing factors are those factors, which result in high correlation with Pearson correlation values of r = 0.988, 0.983, and 0.939, respectively. The study investigates that the most critical factors faced by MSEs are work premises, access to finance, infrastructure, entrepreneurship and business managerial problems. The impact of these factors is found to be high and is confirmed by the 50% drop-out rate in 2014/2015. Furthermore, more than 25% work time losses due to power interruption daily and around 65% work premises problems challenged MSEs. Further, an impact-effort matrix was developed to help the MSEs to prioritize the affecting factors.

  12. Doppler Sonography Confirmation in Patients Showing Calcified Carotid Artery Atheroma in Panoramic Radiography and Evaluation of Related Risk Factors

    Directory of Open Access Journals (Sweden)

    Mahrokh Imanimoghaddam

    2012-02-01

    Full Text Available Background and aims. The purpose of this study was to identify patients at the risk of cerebrovascular attack (CVA by detecting calcified carotid artery atheroma (CCAA in panoramic radiography and evaluating their risk factors. Materials and methods. A total of 960 panoramic radiographs of patients above 40 years old were evaluated. Doppler Sonography (DS was performed for patients who showed calcified carotid artery atheroma (CCAA in panoramic radiography in order to determine the presence of CCAA and the degree of stenosis. Cardiovascular risk factors in both groups of patients with CCAA (12 subjects and without CCAA (3 subjects were compared using a questionnaire filled out by the patients. Statistical analysis including Fisher and independent t-test applied for data analysis. Results. Fifteen patients (30 sides showed calcification in their panoramic radiographs, and underwent DS which revealed CCAA in 16 sides (12 patients. Two patients (13.33% showed stenosis greater than 70%. Among the risk factors, only age showed a significant association with the occurrence of carotid calcified atheroma (P=0.026. Conclusion. Considering the results, dentists should refer especially elderly patients with radiographically identified atheromas for further examinations, as asymptomatic CCAA might be associated with high degrees of stenosis.

  13. Factors secreted from dental pulp stem cells show multifaceted benefits for treating experimental rheumatoid arthritis.

    Science.gov (United States)

    Ishikawa, Jun; Takahashi, Nobunori; Matsumoto, Takuya; Yoshioka, Yutaka; Yamamoto, Noriyuki; Nishikawa, Masaya; Hibi, Hideharu; Ishigro, Naoki; Ueda, Minoru; Furukawa, Koichi; Yamamoto, Akihito

    2016-02-01

    Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial hyperplasia and chronic inflammation, which lead to the progressive destruction of cartilage and bone in the joints. Numerous studies have reported that administrations of various types of MSCs improve arthritis symptoms in animal models, by paracrine mechanisms. However, the therapeutic effects of the secreted factors alone, without the cell graft, have been uncertain. Here, we show that a single intravenous administration of serum-free conditioned medium (CM) from human deciduous dental pulp stem cells (SHED-CM) into anti-collagen type II antibody-induced arthritis (CAIA), a mouse model of rheumatoid arthritis (RA), markedly improved the arthritis symptoms and joint destruction. The therapeutic efficacy of SHED-CM was associated with an induction of anti-inflammatory M2 macrophages in the CAIA joints and the abrogation of RANKL expression. SHED-CM specifically depleted of an M2 macrophage inducer, the secreted ectodomain of sialic acid-binding Ig-like lectin-9 (ED-Siglec-9), exhibited a reduced ability to induce M2-related gene expression and attenuate CAIA. SHED-CM also inhibited the RANKL-induced osteoclastogenesis in vitro. Collectively, our findings suggest that SHED-CM provides multifaceted therapeutic effects for treating CAIA, including the ED-Siglec-9-dependent induction of M2 macrophage polarization and inhibition of osteoclastogenesis. Thus, SHED-CM may represent a novel anti-inflammatory and reparative therapy for RA.

  14. Significant factors for enabling knowledge sharing between government agencies within South Africa

    Directory of Open Access Journals (Sweden)

    Avain Mannie

    2013-07-01

    Full Text Available Background: Globally, organisations have recognised the strategic importance of knowledge management (KM and are increasingly focusing efforts on practices to foster the creation, sharing and integration of knowledge.Objectives: This study aimed to validate the significant factors that influence the effectiveness of KM between government agencies in South Africa. The commonly identified pillars of KM in the extant literature served as a primary framework in establishing these factors.Method: Data were gathered using an electronic survey made available to different national government agencies within the security cluster. Responses were analysed using structural equation modelling.Main findings: Existing literature highlighted organisational culture, learning organisation, collaboration, subject matter experts and trust as being determinants for knowledge management. The first two were identified as the most significant factors for knowledge sharing to succeed.Conclusion: Whilst there is universal consent as to the strategic importance of KM, actionable implementation of knowledge sharing initiatives appears to be lacking. This study emphasised the fact that leaders must instil a knowledge sharing culture either through employee performance contracts or methods such as the balanced score card. The study also showed that it is imperative for leaders to acknowledge that KM is a multi-faceted discipline that offers strategic advantages. Leaders of developing countries should note that they are on a developmental journey. This requires their organisations to be learning organisations, which necessitates a change in the organisational culture and knowledge interventions through their academies of learning.

  15. Self-relation and relations with significant adults as factors of satisfaction with life in adolescents

    Directory of Open Access Journals (Sweden)

    Golovey L.A.

    2017-03-01

    Full Text Available The article is devoted to the study of factors influencing life satisfaction of teenagers raised in families as well as those raised in orphanages. Both groups are studied with regard to their self-attitude, self-confidence and relationships with significant adults. Sample: 96 teenagers aged between 14 and 16 years (46 of them live in or- phanages. Methods: Self-attitude methodology by S.R. Pantileev, Trust questionnaire by T.P. Skripkina, Child-parent relationships questionnaire by O.A. Karabanova and P.V. Troyanskaya, Life Satisfaction scale by E. Diner. The research proved the level of trust in orphanage-raised teenagers to be considerably lower than that of family-raised teenagers. The way teenagers see their relationships with significant adults also turned out to be fairly different in case of orphanage-raised groups due to their caregivers' authoritarian attitude towards them and lack of empathy and communication. The study also shows that the level of life satisfaction in the orphanage group is significantly below the average and substantially lower than in the group from family-raised teenagers. Positive self-attitude, person’s approval of his/ her intellectual capacities, as well as the caregiver's understanding of his pupil's features, are predictors of life satisfaction in the group of teenagers from orphanages. Concerning the family-raised teenagers, much more factors are involved in maintaining their subjective well-being.

  16. [Prognostic significance of serum iron level, hemoglobin and rheumatoid factor titre in rheumatoid arthritis].

    Science.gov (United States)

    Fischer, H; Häntzschel, H; Winiecki, P; Otto, W

    1977-02-01

    On the basis of the results of a five-year examination of the course on 120 patients with rheumatoid arthritis the authors adopt a definite attitude to the prognostic significance of hypersiderinaemia, anaemia and height of the titre of the rheumatoid factor. With the help of the chi2-test and the rank correlation after Spearman the statistical relations to stage, activity, clinical and radiological progressing as well as to the number of the affected joints were examined. In seropositive patients we found a correlation of the titre of rheumatoid factor and stage. Furthermore a clear correlation existed to clinical and radiological progressing as well as to the number of the affected joints. Early highly positive titres of the rheumatoid factor as an expression of high immunologic activity suggest an unfavourable prognosis in the majority of cases. Constant anaemia and hyposiderinaemia as symptoms of a high basis activity of the disease also showed close relations to the progressing. From this result indications for the early use of important therapeutic measures. For the prognostic judgement of the course of the disease of rheumatoid arthritis it is necessary to have at disposal further methodically simply determinable parameters for the recognition of the basis activity and the immunologic activity.

  17. Review of Significant Incidents and Close Calls in Human Spaceflight from a Human Factors Perspective

    Science.gov (United States)

    Silva-Martinez, Jackelynne; Ellenberger, Richard; Dory, Jonathan

    2017-01-01

    This project aims to identify poor human factors design decisions that led to error-prone systems, or did not facilitate the flight crew making the right choices; and to verify that NASA is effectively preventing similar incidents from occurring again. This analysis was performed by reviewing significant incidents and close calls in human spaceflight identified by the NASA Johnson Space Center Safety and Mission Assurance Flight Safety Office. The review of incidents shows whether the identified human errors were due to the operational phase (flight crew and ground control) or if they initiated at the design phase (includes manufacturing and test). This classification was performed with the aid of the NASA Human Systems Integration domains. This in-depth analysis resulted in a tool that helps with the human factors classification of significant incidents and close calls in human spaceflight, which can be used to identify human errors at the operational level, and how they were or should be minimized. Current governing documents on human systems integration for both government and commercial crew were reviewed to see if current requirements, processes, training, and standard operating procedures protect the crew and ground control against these issues occurring in the future. Based on the findings, recommendations to target those areas are provided.

  18. Significance and influence of the ambient temperature as a rate factor of steel reinforcement corrosion

    Indian Academy of Sciences (India)

    V Živica

    2002-10-01

    The rate of corrosion of reinforcement being an electrochemical process, undoubtedly is dependent even on the level of the ambient temperature. Therefore, the ambient temperature seems to be an important factor of the corrosion rate and the durability of the reinforced concrete structures in aggressive environment. The present data on the influence and significance of the ambient temperature in the process of corrosion of reinforcement of the reinforced structures are surprisingly limited and poor. It seems that it is supposed to be a simple increase of corrosion rate when the ambient temperature is increased. The lack of information was a motivation for the present study. It was aimed at the experimental research of the influence of the increase of the ambient temperature on the rate of chloride induced corrosion of steel reinforcement. The results obtained show that the influence of the studied factor is more complex showing an acceleration effect till a temperature of 40°C diversified by the inhibition effects with further increase of the ambient temperature.

  19. Live Reporting in a News / Current Affairs TV Show as a Factor of (Non Credibility

    Directory of Open Access Journals (Sweden)

    Tena Perišin

    2010-12-01

    Full Text Available Only a perfunctory glance at the content of current aff airs/news programs makes it clear that in just a few years time, live reporting on Croatian TV news shows has increased dramatically. Technologicaladvancements, the equipping of TV stations with mobile satellite vans, and the pressure of competition have all resulted in increased instances of live reporting. When investigating news values, pictureand sound, as well as the possibility of immediate, timely reporting represent the key characteristics of TV journalism. In this context, live reports, as part of a news segment, should add to the authenticity and credibility of the program. Currently, however, TV broadcasting houses attempt to best one another in the number of live broadcasts as a means to purport a higher quality of their program. The direct address to the camera turns a reporter from an anonymous bearer of information into the “main star”. The figures accumulated on the extent of this form used in a news show do not determine the professional level of the editorial policy. Live reporting cannot be regarded as news value if other news values are neglected in the process. Recent research shows that live reports, in most cases, have been stripped of the initial notion of reporting on important and recent events. As such, they are becoming less of a justifi ed element in the creation of news.

  20. Natural and anthropogenic factors which inf luence aerosol distribution in Ingleborough Show Cave, UK

    Directory of Open Access Journals (Sweden)

    Smith Andrew C.

    2013-01-01

    Full Text Available Monitoring in Ingleborough Show Cave (N. Yorkshire, UK reveals the inf luence of tourism and cave management techniques on different parameters of the cave atmosphere. Exploratory aerosol monitoring identif ied a 0.015 ± 0.03 mg/m3 (≈70% reduction in airborne particulates within the f irst 75 meters of cave passage and two major aerosol sources within this artif icially ventilated show cave. Autogenic aerosol production was identif ied close to active stream ways (increases of <0.012 mg/m3, suggesting the expulsion of water-borne aerosols during turbulent water f low. The presence of tourist groups also increased aerosol concentrations within the cave (increases of <0.021 mg/m3, either by transporting them from an allogenic source or through the disturbance of particles which had previously been deposited within the cave environment. Exploratory aerosol data is presented alongside more routine analytical monitoring, helping to contextualise the impact of cave management strategies on natural cave atmospherics.

  1. Risk Factors for Clinically Significant Intimate Partner Violence among Active-Duty Members

    Science.gov (United States)

    Smith Slep, Amy M.; Foran, Heather M.; Heyman, Richard E.; Snarr, Jeffery D.

    2011-01-01

    Hypothesized risk factors for men's and women's clinically significant intimate partner violence (CS-IPV) from four ecological levels (i.e., individual, family, workplace, community) were tested in a representative sample of active-duty U.S. Air Force members (N = 42,744). When considered together, we expected only individual and family factors to…

  2. Randomised control trial showed that delayed cord clamping and milking resulted in no significant differences in iron stores and physical growth parameters at one year of age.

    Science.gov (United States)

    Agarwal, Shivam; Jaiswal, Vijay; Singh, Dharamveer; Jaiswal, Prateek; Garg, Amit; Upadhyay, Amit

    2016-11-01

    Placental redistribution has been shown to improve haematological outcomes in the immediate neonatal period and early infancy. This study compared the effects of delayed cord clamping (DCC) and umbilical cord milking (UCM) on haematological and growth parameters at 12 months of age. This was a follow-up study of a randomised control trial, conducted in a tertiary care paediatric centre from August 2013 to August 2014. We studied 200 apparently healthy Indian infants randomised at birth to receive DCC for 60-90 seconds or UCM. The outcome measures were iron status and physical growth parameters at 12 months. Of the 200 babies, 161 completed the follow-up and baseline characteristics were comparable in both groups. The mean haemoglobin in the DCC group (102.2 (17.2) g/L and serum ferritin 16.44 (2.77) μg/L) showed no significant differences to the UCM group (98.6 (17.1) g/L and 18.2 (2.8) μg/L) at one year. In addition, there were no significant differences in weight, height and mid-upper arm circumference in the two groups. Term-born Indian infants who had DCC at 60-90 seconds or UCM showed no significant differences in ferritin and haemoglobin levels and growth parameters at 12 months of age. ©2016 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.

  3. Interdisciplinarity, Debate And Movie Clips As Highly Motivating Factors In Live Shows - Five Years Of Success

    Science.gov (United States)

    Stengler, E.; Sirera, J. M.

    2011-09-01

    A live show on any subject that includes experiments and continuous interaction with the audience is a well known approach for EPO activities that many are carrying out all over. We present such an initiative with some added ingredients such as interdisciplinarity, the use of movie clips, and especially the debate between the two presenters, a debate that is all the more attractive to the public if it not fully staged but closely represents their actual points of view. José Montesinos, from the "Orotava" Canarian Foundation for the History of Science, is and plays the role of the more mature math professor who has grown weary of the overrated value given in science to mathematics and its consequences. This poses a constant challenge to his colleague, Erik Stengler, from the Science Museum of Tenerife, the young down-to-earth hands-on scientist, who defends the usual view that science and technology are to be judged by their achievements, which have brought about the advancement of modern society. With this approach and as a collaboration between our institutions, we have produced and toured highly successful activities on: Einstein and Relativity (from 2005 to 2008, "Einstein Goes To School," including a theatre play); circularity, the number π, forces of inertia and the Newtonian revolution (in 2008/2009, "The Tension Between Circularity and The Straight Line"); and the foundations of modern astronomy (in 2009/2010 "Kepler and Galileo, Messengers of the Stars"). Audiences were very varied - students, adult students, general public, prison inmates, teachers - and all appreciated the presentations as fun, thought-provoking and highly motivating, and valued especially the interdisciplinary character of the activity. Movie clips have shown to be especially useful to recover the attention of the young when they lose the thread due to the short attention spans they presently have.

  4. Gedunin and photogedunin of Xylocarpus granatum show significant anti-secretory effects and protect the gastric mucosa of peptic ulcer in rats.

    Science.gov (United States)

    Lakshmi, V; Singh, N; Shrivastva, S; Mishra, S K; Dharmani, P; Mishra, V; Palit, G

    2010-07-01

    In the present study, the gastroprotective mechanism of Xylocarpus granatum fruit and its active constituents gedunin and photogedunin was investigated. Chloroform fraction (Fr-CHCl(3)) of X. granatum fruit was evaluated against cold restraint (CRU), aspirin (AS), alcohol (AL) and pyloric ligation (PL) induced gastric ulcer models in rats and histamine (HA) induced duodenal ulcer model in guinea pigs. Potential anti-ulcer activity of Fr-CHCl(3) was observed against CRU (58.28%), AS (67.81%), AL (84.38%), PL (65.66%) and HA (61.93%) induced ulcer models. The standard drug omeprazole (10mg/kg, p.o.) showed 68.25% protection against CRU, 57.08% against AS and 69.42% against PL model and 70.79% against HA induced duodenal ulcer. Sucralfate, another standard drug (500 mg/kg, p.o.) showed 62.72% protection in AL induced ulcer model. Fr-CHCl(3) significantly reduced free acidity (51.42%), total acidity (30.76%) and upregulated mucin secretion by 58.37% respectively. Phytochemical investigations of Fr-CHCl(3) yielded gedunin (36%), photogedunin (2%). Further, Fr-CHCl(3) and its compounds gedunin and photogedunin significantly inhibited H(+) K(+)-ATPase activity in vitro with IC(50) of 89.37, 56.86 and 66.54 microg/ml respectively as compared to the IC(50) value of omeprazole (30.24 microg/ml) confirming their anti-secretory activity. Conclusively, Fr-CHCl(3) of Xylocarpus granatum was found to possess anti-ulcerogenic activity which might be due to its anti-secretory activity and subsequent strengthening of the defensive mechanism. This study is the first of its kind to show significant anti-secretory effect of gedunin and photogedunin. Therefore it could act as a potent therapeutic agent against peptic ulcer disease.

  5. Identifying risk factors for clinically significant diabetic macula edema in patients with type 2 diabetes mellitus.

    Science.gov (United States)

    Kamoi, Kyuzi; Takeda, Keiji; Hashimoto, Kaoru; Tanaka, Reiko; Okuyama, Shinya

    2013-05-01

    It is known that clinic blood pressure (BP), gender, cigarette smoking, dyslipidemia, anemia and thiazolidenediones (TZD) treatment are predictors for clinically significant diabetic macula edema (CSDME). We examined a most risky factor for CSDME in Japanese patients with type 2 diabetes mellitus (T2DM) and diabetic retinopathy (DR) confirmed using optical coherence tomography by multiple regression analysis (MRA). As the risk factors, wakening-up BP was added to such factors. Seven diabetic Japanese patients with CSDME (group 1) and 124 subjects without CSDME (group 2) assonated with DR using optical coherence tomography were studied. The durations of T2DM in groups 1 and 2 were 15±10 years and 20±15 years, respectively. There was no statistically difference in means of gender, duration, age, body mass index (BMI), HbA1c, TC, LDL and TC/HDL, serum creatinine, urinary albumin excretion rate, and clinic BP between two groups. Morning systolic home BP (MSHBP), cigarette smoking and foveal thickness were significantly (ppioglitazone as TZD treatment were significantly positive predictors for CSDME, while BMI had a significantly negative predictor. Other variables were not significantly correlated to CSDME. The review summarizes a multiple regression analysis revealed that MSHBP makes an addition to predictive factors for CSDME among risk factors reported previously in patient with T2DM.

  6. Significant blockade of multiple receptor tyrosine kinases by MGCD516 (Sitravatinib), a novel small molecule inhibitor, shows potent anti-tumor activity in preclinical models of sarcoma.

    Science.gov (United States)

    Patwardhan, Parag P; Ivy, Kathryn S; Musi, Elgilda; de Stanchina, Elisa; Schwartz, Gary K

    2016-01-26

    Sarcomas are rare but highly aggressive mesenchymal tumors with a median survival of 10-18 months for metastatic disease. Mutation and/or overexpression of many receptor tyrosine kinases (RTKs) including c-Met, PDGFR, c-Kit and IGF1-R drive defective signaling pathways in sarcomas. MGCD516 (Sitravatinib) is a novel small molecule inhibitor targeting multiple RTKs involved in driving sarcoma cell growth. In the present study, we evaluated the efficacy of MGCD516 both in vitro and in mouse xenograft models in vivo. MGCD516 treatment resulted in significant blockade of phosphorylation of potential driver RTKs and induced potent anti-proliferative effects in vitro. Furthermore, MGCD516 treatment of tumor xenografts in vivo resulted in significant suppression of tumor growth. Efficacy of MGCD516 was superior to imatinib and crizotinib, two other well-studied multi-kinase inhibitors with overlapping target specificities, both in vitro and in vivo. This is the first report describing MGCD516 as a potent multi-kinase inhibitor in different models of sarcoma, superior to imatinib and crizotinib. Results from this study showing blockade of multiple driver signaling pathways provides a rationale for further clinical development of MGCD516 for the treatment of patients with soft-tissue sarcoma.

  7. A dual acting compound releasing nitric oxide (NO) and ibuprofen, NCX 320, shows significant therapeutic effects in a mouse model of muscular dystrophy.

    Science.gov (United States)

    Sciorati, Clara; Miglietta, Daniela; Buono, Roberta; Pisa, Viviana; Cattaneo, Dario; Azzoni, Emanuele; Brunelli, Silvia; Clementi, Emilio

    2011-09-01

    A resolutive therapy for muscular dystrophies, a heterogeneous group of genetic diseases leading to muscular degeneration and in the severe forms to death, is still lacking. Since inflammation and defects in nitric oxide generation are recognized key pathogenic events in muscular dystrophy, we have analysed the effects of a derivative of ibuprofen, NCX 320, belonging to the class of cyclooxygenase inhibiting nitric oxide donator (CINOD), in the α-sarcoglycan null mice, a severe mouse model of dystrophy. NCX 320 was administered daily in the diet for 8months starting 1month from weaning. Muscle functional recovery was evaluated by free wheel and treadmill tests at 8months. Serum creatine kinase activity, as well as the number of diaphragm inflammatory infiltrates and necrotic fibres, was measured as indexes of skeletal muscle damage. Muscle regeneration was evaluated in diaphragm and tibialis anterior muscles, measuring the numbers of centronucleated fibres and of myogenic precursor cells. NCX 320 mitigated muscle damage, reducing significantly serum creatine kinase activity, the number of necrotic fibres and inflammatory infiltrates. Moreover, NCX 320 stimulated muscle regeneration increasing significantly the number of myogenic precursor cells and regenerating fibres. All these effects concurred in inducing a significant improvement of muscle function, as assessed by both free wheel and treadmill tests. These results describe the properties of a new compound incorporating nitric oxide donation together with anti-inflammatory properties, showing that it is effective in slowing muscle dystrophy progression long term. Of importance, this new compound deserves specific attention for its potential in the therapy of muscular dystrophy given that ibuprofen is well tolerated in paediatric patients and with a profile of safety that makes it suitable for chronic treatment such as the one required in muscular dystrophies.

  8. Identification of the potential risk factors for monoclonal gammopathy of undetermined significance of progression.

    Science.gov (United States)

    Li, Rong; Du, Juan; Hou, Jian

    2015-01-01

    Monoclonal gammopathy of undetermined significance (MGUS) is a premalignant plasma cell disorder. The etiology of MGUS progression remains unclear and is a current topic of investigation. This review summarizes the essential features of MGUS and the potential risk factors for MGUS of progression. Many clinical studies have been conducted to identify the critical risk markers that play important roles in progression. Some clinical variables, such as immunophenotypic markers and cytogenetic changes, have been recognized as potential risk factors. In this review, we discuss novel insights from recent studies of potential risk factors, and we propose future directions for clinical management and additional studies.

  9. Detection and significance of inflammatory factors and immunoglobulin in acute stage patients with infantile pneumonia

    Institute of Scientific and Technical Information of China (English)

    Xiao-Zheng Meng; Liang Qiao

    2016-01-01

    Objective:To investigate the detection and significance of inflammatory factors and immunoglobulin in acute stage patients with infantile pneumonia.Methods:A total of 80 cases of acute stage patients with infantile pneumonia were divided into mild group (n=33) and severe group (n=47) according to illness degrees, and 40 cases of healthy children were selected as control group. Serum CRP, Ig A, Ig G and Ig M levels were detected by nephelometry, IL-6 levels were detected by enzyme-linked immunosorbent assay. The levels of inflammatory factors (CRP, IL-6) and immunoglobulin indexes (Ig A, Ig G and Ig M) were compared among 3 groups.Results: The inflammatory factors (CRP, IL-6) in mild and severe group increased significantly compared with control group, CRP and IL-6 levels in severe group were were significantly higher than that in mild group (P<0.05). The levels of Ig A in mild and severe group decreased significantly compared with control group, Ig A level in severe group was significantly lower than that in mild group (P<0.05), the levels of Ig G and Ig M in mild and severe group increased significantly compared with control group, the levels of Ig G and Ig M in severe group were significantly higher than that in mild group (P<0.05). Conclusions:The levels of inflammatory factors are increased while immune function is decreased in acute stage patients with infantile pneumonia. Inflammation is stronger and immune function is worse with the severity of the disease. Detection of inflammatory factors and immunoglobulin levels is helpful to diagnose and treatment of acute stage patients with infantile pneumonia.

  10. Hypertriglyceridemia in an outpatient department--Significance as an atherosclerotic risk factor.

    Science.gov (United States)

    Scholz, M; Tselmin, S; Fischer, S; Julius, U

    2015-05-01

    Although a relationship between elevated triglycerides (TG) and cardiovascular diseases is generally accepted, its extent is still discussed. This retrospective study analyzed the incidence of cardiovascular events (CVE) and pancreatitis as well as the therapeutic regimen in patients being treated for hypertriglyceridemia (HTG) at an outpatient department. The cohort included 183 patients with mild and 49 patients with severe HTG; subgroups were formed and compared according to gender, presence of metabolic vascular syndrome (MVS) and lipid values. Patients in this study seem to have had CVE at younger age than reported event rates in the general population. TG levels, rates of CVE and pancreatitis were reduced in all groups during therapy, which could be linked to use of omega-3 fatty acids and fibrates. Patients with persisting severe HTG as a result of incompliance showed massive risk for pancreatitis. Although no significant association between TG levels and CVE could be established, the combination of HTG and other cardiovascular risk factors such as MVS seems to be especially dangerous. The lipid-lowering drug therapy appeared to be effective with respect to CVE and pancreatitis incidence. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  11. Coping with perceived racism: a significant factor in the development of obesity in African American women?

    Science.gov (United States)

    Mwendwa, Denee T; Gholson, Georica; Sims, Regina C; Levy, Shellie-Anne; Ali, Mana; Harrell, C Jules; Callender, Clive O; Campbell, Alfonso L

    2011-07-01

    African American women have the highest rates of obesity in the United States. The prevalence of obesity in this group calls for the identification of psychosocial factors that increase risk. Psychological stress has been associated with obesity in women; however, there is scant literature that has explored the impact of racism on body mass index (BMI) in African American women. The current study aimed to determine whether emotional responses and behavioral coping responses to perceived racism were associated with BMI in African American women. A sample of 110 African American women participated in a community-based study. Height and weight measurements were taken to calculate BMI and participants completed the Perceived Racism Scale and the Perceived Stress Scale. Hierarchical regression analyses demonstrated a significant relationship between BMI and behavioral coping responses to perceived racism. Findings for emotional responses to perceived racism and appraisal of one's daily life as stressful were nonsignificant. Mean comparisons of BMI groups showed that obese African American women used more behavioral coping responses to perceived racism as compared to normal-weight and overweight women in the sample. Findings suggest that behavioral coping responses better explained increased risk for obesity in African American women. A biobehavioral pathway may explain this finding with a stress-response process that includes cortisol reactivity. Maladaptive behavioral coping responses may also provide insight into obesity risk. Future research is needed to determine which behavioral coping responses place African American women at greater risk for obesity.

  12. Yearly variation of spontaneous somatic mutation frequency in the stamen hairs of Tradescantia clone KU 9 grown outdoors, which showed a significant increase after the Chernobyl accident.

    Science.gov (United States)

    Ichikawa, S; Nakano, A; Kenmochi, M; Yamamoto, I; Murai, M; Takahashi, E; Yamaguchi, A; Watanabe, K; Tomiyama, M; Sugiyama, K; Yogo, A; Yazaki, T; Okumura, M; Shima, N; Satoh, M; Yoshimoto, M; Xiao, L Z

    1996-02-01

    Scoring of spontaneous somatic pink mutation frequency in the stamen hairs of Tradescantia clone KU 9, a heterozygote for flower color (blue/pink; the blue color being dominant), was carried out for 11 years on plants grown outdoors, during the period of May 11-31 (for 3 weeks) in every year from 1982 to 1992. Weekly and yearly variations of the spontaneous mutation frequency were observed, and such variations could mostly be correlated to the difference in temperature. That is, the mutation frequency was generally higher in the weeks and years when the temperature was relatively low, showing the strongest negative correlation with the average minimum temperature. The variations were also correlated to the diurnal temperature difference, the mutation frequency being higher with larger diurnal temperature difference in general. However, the mutation frequency observed in 1986 was exceptionally higher than that expected from the temperature for this year, and was very significantly higher than for other years. The scoring of mutation frequency was thus continued in 1986 for an additional 4 weeks (June 1-28), and it was confirmed that such higher mutation frequencies lasted for 6 weeks in total. The exceptionally high mutation frequency seemed to be related to the radioactive fallout which occurred in early to mid May of 1986, even in Japan, after the serious nuclear reactor accident at Chernobyl, and also to the biological concentrations of radioactive nuclides which subsequently occurred, although it was difficult to conclude this definitely. The mutation frequency in 1987 was second highest, and was also significantly higher than the lowest mutation frequency observed in 1990.

  13. The changes and significance of serum inflammatory factors and hemodynamics in patients with acute cerebral infarction

    Institute of Scientific and Technical Information of China (English)

    Xiao-Wei Lu

    2016-01-01

    Objective:To investigate the changes of serum inflammatory factors and hemodynamics in patients with acute cerebral infarction and its clinical significance.Methods: A total of 55 cases of acute cerebral infarction (ACI) patients as observation group, and cases of healthy physical examination were selected as the observation group, and 55 healthy persons as control group. ELISA method was used to detect inflammatory cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), C-reactive protein (CRP) and tumor necrosis factor (TNF-α) level, WA-880 heart and brain integrated digital hemodynamic monitor to detect bilateral carotid artery blood flow velocity, blood flow and peripheral resistance.Results:The serum levels of IL-8, CRP, IL-6 and TNF-α were higher in the observation group than in the control group, the difference was statistically significant (P<0.05). The blood flow velocity and blood flow velocity in the observation group were significantly lower than those in the control group. The difference was statistically significant (IL-8). With the increase of infarct size, serum IL-6, CRP,P<0.05 and TNF-α increased significantly (P<0.05).Conclusions:The changes of serum inflammatory factors and hemodynamic indexes can be used to judge the early cerebral infarction and the size of the infarct size of the index, the clinical dynamic monitoring of its changes in patients with acute cerebral infarction and the severity of the prognosis and the prognosis of the important significance of the judgment.

  14. An Ecological-Transactional Model of Significant Risk Factors for Child Psychopathology in Outer Mongolia

    Science.gov (United States)

    Kohrt, Holbrook E.; Kohrt, Brandon A.; Waldman, Irwin; Saltzman, Kasey; Carrion, Victor G.

    2004-01-01

    The present study examined significant risk factors, including child maltreatment, for child psychopathology in a cross-cultural setting. Ninety-nine Mongolian boys, ages 3-10 years, were assessed. Primary caregivers (PCG) completed structured interviews including the Emory Combined Rating Scale (ECRS) and the Mood and Feelings Questionnaire…

  15. The Identification of Risk Factors: the Control of the Significance Level in Multiple Comparisons

    Directory of Open Access Journals (Sweden)

    Stefania Naddeo

    2007-10-01

    Full Text Available A common problem in statistical medical analyses is the identification of risk factors associated with a certain disease. The collected sampled data are used to assess simultaneously more hypotheses, each of which assesses the influence of one factor. It is well known that the simultaneous assessment of two or more hypotheses entails a rise in the probability of rejecting at least one of the true null partial hypotheses. In this paper the rise in this probability is approximately evaluated for different levels of dependance between factors by means of a permutation-based procedure. The paper also proposes a procedure which computes an adjusted p-value for each test of the partial hypotheses in such a way that the global hypothesis that none of the factors have influence is assessed at a prefixed significance level. A simulation study is performed to check the power of the proposed procedure.

  16. The coronary calcium score is a more accurate predictor of significant coronary stenosis than conventional risk factors in symptomatic patients

    DEFF Research Database (Denmark)

    Nicoll, R; Wiklund, U; Zhao, Y;

    2016-01-01

    risk factor assessment, computed tomographic coronary angiogram (CTCA) or conventional angiography and a CT scan for coronary artery calcium (CAC) scoring. 1539 (27.9%) patients had significant stenosis, 5.5% of whom had zero CAC. In 5074 patients, multiple binary regression showed the most important...... predictor of significant stenosis to be male gender (B=1.07) followed by diabetes mellitus (B=0.70) smoking, hypercholesterolaemia, hypertension, family history of CAD and age but not obesity. When the log transformed CAC score was included, it became the most powerful predictor (B=1.25), followed by male...... gender (B=0.48), diabetes, smoking, family history and age but hypercholesterolaemia and hypertension lost significance. The CAC score is a more accurate predictor of >50% stenosis than risk factors regardless of the means of assessment of stenosis. The sensitivity of risk factors, CAC score...

  17. Clinical significance of vascular endothelial growth factor expression and neovascularization in colorectal carcinoma

    Institute of Scientific and Technical Information of China (English)

    Shu Zheng; Ming-Yong Han; Zuo-Xiang Xiao; Jia-Ping Peng; Qi Dong

    2003-01-01

    AIM: To clarify the association of vascular endothelial growth factor (VEGF) and microvascular density (MVD)expression with the angiogenesis and prognosis of colorectal cancer.METHODS: A total of 97 cases of colorectal carcinomas were examined by immunohistochemical staining (SP method), using anti-VEGF and anti-factor CD34+ monoclonal antibodies. RESULTS: VEGF positive staining was obtained in 68 out of 97 cases (70.1%), and observed mainly in the cytoplasm of tumor cells, and also frequently in stromal cells. VEGF expression was more intense in poorly differentiated adenocarcinoma in comparison with others, but there was no significant correlation between VEGF expression and age,sex and stage. A significant correlation was found between the MVD and grades, and there was no significant relationship between the MVD and age, sex, and stage. The MVD in the VEGF positive group (68 cases) was higher than that in the negative group. Upon multivariate analysis, the significant variables were stage, tumor grade and MVD; VEGF expression was not an independent prognostic factor. CONCLUSION: The expression of VEGF has a significant correlation with MVD; MVD expression has prognostic value but VEGF has not in colon cancer.

  18. Research report on the physiological effects of air ions and their significance as environmental factors

    Science.gov (United States)

    Varga, A.

    1978-01-01

    The series of experiments performed have shown that small air ions generated artificially using radioactive materials produced physiological effects in all test subjects, which are described. These results show that the air ions were important climatic factors in the production of comfortable and healthy room climates.

  19. A randomized trial in a massive online open course shows people don’t know what a statistically significant relationship looks like, but they can learn

    Directory of Open Access Journals (Sweden)

    Aaron Fisher

    2014-10-01

    Full Text Available Scatterplots are the most common way for statisticians, scientists, and the public to visually detect relationships between measured variables. At the same time, and despite widely publicized controversy, P-values remain the most commonly used measure to statistically justify relationships identified between variables. Here we measure the ability to detect statistically significant relationships from scatterplots in a randomized trial of 2,039 students in a statistics massive open online course (MOOC. Each subject was shown a random set of scatterplots and asked to visually determine if the underlying relationships were statistically significant at the P < 0.05 level. Subjects correctly classified only 47.4% (95% CI [45.1%–49.7%] of statistically significant relationships, and 74.6% (95% CI [72.5%–76.6%] of non-significant relationships. Adding visual aids such as a best fit line or scatterplot smooth increased the probability a relationship was called significant, regardless of whether the relationship was actually significant. Classification of statistically significant relationships improved on repeat attempts of the survey, although classification of non-significant relationships did not. Our results suggest: (1 that evidence-based data analysis can be used to identify weaknesses in theoretical procedures in the hands of average users, (2 data analysts can be trained to improve detection of statistically significant results with practice, but (3 data analysts have incorrect intuition about what statistically significant relationships look like, particularly for small effects. We have built a web tool for people to compare scatterplots with their corresponding p-values which is available here: http://glimmer.rstudio.com/afisher/EDA/.

  20. A randomized trial in a massive online open course shows people don't know what a statistically significant relationship looks like, but they can learn.

    Science.gov (United States)

    Fisher, Aaron; Anderson, G Brooke; Peng, Roger; Leek, Jeff

    2014-01-01

    Scatterplots are the most common way for statisticians, scientists, and the public to visually detect relationships between measured variables. At the same time, and despite widely publicized controversy, P-values remain the most commonly used measure to statistically justify relationships identified between variables. Here we measure the ability to detect statistically significant relationships from scatterplots in a randomized trial of 2,039 students in a statistics massive open online course (MOOC). Each subject was shown a random set of scatterplots and asked to visually determine if the underlying relationships were statistically significant at the P < 0.05 level. Subjects correctly classified only 47.4% (95% CI [45.1%-49.7%]) of statistically significant relationships, and 74.6% (95% CI [72.5%-76.6%]) of non-significant relationships. Adding visual aids such as a best fit line or scatterplot smooth increased the probability a relationship was called significant, regardless of whether the relationship was actually significant. Classification of statistically significant relationships improved on repeat attempts of the survey, although classification of non-significant relationships did not. Our results suggest: (1) that evidence-based data analysis can be used to identify weaknesses in theoretical procedures in the hands of average users, (2) data analysts can be trained to improve detection of statistically significant results with practice, but (3) data analysts have incorrect intuition about what statistically significant relationships look like, particularly for small effects. We have built a web tool for people to compare scatterplots with their corresponding p-values which is available here: http://glimmer.rstudio.com/afisher/EDA/.

  1. A randomized trial in a massive online open course shows people don’t know what a statistically significant relationship looks like, but they can learn

    Science.gov (United States)

    Fisher, Aaron; Anderson, G. Brooke; Peng, Roger

    2014-01-01

    Scatterplots are the most common way for statisticians, scientists, and the public to visually detect relationships between measured variables. At the same time, and despite widely publicized controversy, P-values remain the most commonly used measure to statistically justify relationships identified between variables. Here we measure the ability to detect statistically significant relationships from scatterplots in a randomized trial of 2,039 students in a statistics massive open online course (MOOC). Each subject was shown a random set of scatterplots and asked to visually determine if the underlying relationships were statistically significant at the P < 0.05 level. Subjects correctly classified only 47.4% (95% CI [45.1%–49.7%]) of statistically significant relationships, and 74.6% (95% CI [72.5%–76.6%]) of non-significant relationships. Adding visual aids such as a best fit line or scatterplot smooth increased the probability a relationship was called significant, regardless of whether the relationship was actually significant. Classification of statistically significant relationships improved on repeat attempts of the survey, although classification of non-significant relationships did not. Our results suggest: (1) that evidence-based data analysis can be used to identify weaknesses in theoretical procedures in the hands of average users, (2) data analysts can be trained to improve detection of statistically significant results with practice, but (3) data analysts have incorrect intuition about what statistically significant relationships look like, particularly for small effects. We have built a web tool for people to compare scatterplots with their corresponding p-values which is available here: http://glimmer.rstudio.com/afisher/EDA/. PMID:25337457

  2. Comprehensive analysis of clinical significance of stem-cell related factors in renal cell cancer

    Directory of Open Access Journals (Sweden)

    Zhou Libin

    2011-10-01

    Full Text Available Abstract Background C-MYC, LIN28, OCT4, KLF4, NANOG and SOX2 are stem cell related factors. We detected whether these factors express in renal cell carcinoma (RCC tissues to study their correlations with the clinical and pathological characteristics. Methods The expressions of c-MYC, LIN28, SOX2, KLF4, OCT4 and NANOG in 30 RCC patients and 5 non-RCC patients were detected with quantitative real-time reverse transcription-PCR (qRT-PCR. The data were analyzed with Wilcoxon signed rank sum test and x2 test. Results In RCC group, c-MYC expression was significantly higher in RCC tissues compared with normal tissues (P 0.05. Also the expression levels of all above factors were not significantly changed in non-RCC group (P > 0.05. Conclusions The present analysis strongly suggests that altered expression of several stem cell related factors may play different roles in RCC. C-MYC may function as an oncogene and OCT4, KLF4, NANOG and SOX2 as tumor suppressors.

  3. Expression and clinical significance of fibroblast growth factor 1 in gastric adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Liu NQ

    2015-03-01

    Full Text Available Naiqing Liu,1,2,* Jingyu Zhang,2,* Shuxiang Sun,2 Liguang Yang,2 Zhongjin Zhou,2 Qinli Sun,2 Jun Niu11Department of General Surgery, Qilu Hospital Affiliated to Shandong University, Jinan, People’s Republic of China; 2Department of General Surgery, Yishui Central Hospital, Linyi, People’s Republic of China*These authors contributed equally to this workBackground: The clinical significance of fibroblast growth factor 1 (FGF1 has been revealed in several cancers, including ovarian cancer, breast cancer, and bladder cancer. However, the clinical significance of FGF1 in gastric adenocarcinoma has not been explored.Patients and methods: In our experiments, we systematically evaluated FGF1 expression in 178 cases of gastric adenocarcinoma with immunohistochemistry, and subsequently analyzed the correlation between FGF1 expression and clinicopathologic features. Moreover, FGF1 expression in tumor tissue and corresponding adjacent tissue was detected and compared by real-time polymerase chain reaction. The Kaplan–Meier method and the Cox-regression model were used with univariate and multivariate analysis, respectively, to evaluate the prognostic value of FGF1 in gastric adenocarcinoma.Results: Higher FGF1 expression rate is 56.7% (101/178 in gastric adenocarcinoma. FGF1 expression in gastric adenocarcinoma was significantly higher than adjacent tissue (P<0.0001. Expression of FGF1 is significantly associated with lymph node invasion (P<0.001, distant metastasis (P=0.013, and differentiation (P=0.015. Moreover, FGF1 overexpression was closely related to unfavorable overall survival rate (P=0.021, and can be identified to be an independent unfavorable prognostic factor (P=0.004.Conclusion: FGF1 is an independent prognostic factor, indicating that FGF1 could be a potential molecular drug target in gastric adenocarcinoma.Keywords: fibroblast growth factor 1, gastric adenocarcinoma, prognosis, biomarker, lymph node, gene fusion

  4. The significance of psychosocial factors of the working environment in the development of sick building syndrome

    Directory of Open Access Journals (Sweden)

    Maja Miškulin

    2014-12-01

    Full Text Available Introduction: Sick building syndrome (SBS is a medical condition in which people in a certain buildings suffer from symptoms of illness or feeling unwell. The aim of this study was to determine the frequency of exposure of the employees of public institutions from the city of Osijek to harmful psychosocial factors of the working environment, to assess whether there is a connection between the exposure to these factors and the incidence of SBS symptoms and to clarify the nature of this connection.Methods: This cross-sectional study was conducted during May 2013 among 178 employees of public institutions in the city of Osijek. An anonymous questionnaire which contained questions relating to demographic data and working status of the participants, their exposure to various harmful psychosocial factors of the working environment and occurrence of certain symptoms of SBS among them was used as a research tool.Results: 96.1 % (171/178 of participants were exposed to harmful psychosocial factors of the working environment. Employees exposed to those factors more frequently expressed symptoms of SBS. The incidence and the number of symptoms of SBS among employees simultaneously grew with the increase of the number of harmful psychosocial factors of the working environment to which they were exposed.Conclusion: The study showed positive connection between the exposure to harmful psychosocial factors of the working environment and the incidence of SBS symptoms, highlighting this issue as a very important subject in the field of occupational medicine and health protection in the workplace.

  5. Significant factors of aviation insurance and risk management strategy: an empirical study of Taiwanese airline carriers.

    Science.gov (United States)

    Lin, Yi Hsin; Chang, Yu Hern

    2008-04-01

    Aviation insurance premiums have become a heavy burden for the airline industry since September 11, 2001. Although the industry must constantly balance its operations between profitability and safety, the reality is that airlines are in the business of making money. Therefore, their ability to reduce cost and manage risk is a key factor for success. Unlike past research, which used subjective judgment methods, this study applied quantitative historical data (1999-2000) and gray relation analysis to identify the primary factors influencing ratemaking for aviation insurance premiums. An empirical study of six airlines in Taiwan was conducted to determine these factors and to analyze the management strategies used to deal with them. Results showed that the loss experience and performance of individual airlines were the key elements associated with aviation insurance premiums paid by each airline. By identifying and understanding the primary factors influencing ratemaking for aviation insurance, airlines will better understand their relative operational strengths and weaknesses, and further help top management identify areas for further improvement. Knowledge of these factors combined with effective risk management strategies, may result in lower premiums and operating costs for airline companies.

  6. PERSONAL AND ENVIRONMENTAL RISK FACTORS SIGNIFICANTLY ASSOCIATED WITH ELEVATED BLOOD LEAD LEVELS IN RURAL THAI CHILDREN.

    Science.gov (United States)

    Swaddiwudhipong, Witaya; Kavinum, Suporn; Papwijitsil, Ratchadaporn; Tontiwattanasap, Worawit; Khunyotying, Wanlee; Umpan, Jiraporn; BoonthuM, Ratchaneekorn; Kaewnate, Yingyot; Boonmee, Sasis; Thongchub, Winai; Rodsung, Thassanee

    2014-11-01

    A community-based study was conducted to determine personal risk factors and environmental sources of lead exposure for elevated blood lead levels (≥ 10 µg/dl, EBLLs) among rural children living at the Thailand-Myanmar border in Tak Province, northwestern Thailand. Six hundred ninety-five children aged 1-14 years old were screened for BLLs. Environmental specimens for lead measurements included samples of water from the streams, taps, and household containers, house floor dust, and foods. Possible lead release from the cooking ware was determined using the leaching method with acetic acid. The overall prevalence of EBLLs was 47.1% and the geometric mean level of blood lead was 9.16 µg/dl. Personal risk factors significantly associated with EBLLs included being male, younger age, anemia, and low weight-for-age. Significant environmental risk factors were exposure to a lead-acid battery of solar energy system and use of a non-certified metal cooking pot. Some families whose children had high BLLs reported production of lead bullets from the used batteries at home. About one-third of the house dust samples taken near batteries contained lead content above the recommended value, compared with none of those taken from other areas and from the houses with no batteries. The metal pots were safe for cooking rice but might be unsafe for acidic food preparation. Both nutritional intervention and lead exposure prevention programs are essential to reduce EBLLs in this population.

  7. Significant factors associated with fatal outcome in emergency open surgery for perforated peptic ulcer

    Institute of Scientific and Technical Information of China (English)

    Mario Testini; Piero Portincasa; Giuseppe Piccinni; Germana Lissidini; Fabio Pellegrini; Luigi Greco

    2003-01-01

    AIM: To evaluate the main factors associated with mortality in patients undergoing surgery for perforated peptic ulcer referred to an academic department of general surgery in a large southern Italian city.METHODS: One hundred and forty-nine consecutive patients (M:F ratio=110:39, mean age 52 yrs, range 16-95) with peptic ulcer disease were investigated for clinical history (including age, sex, previous history of peptic ulcer, associated diseases, delayed abdominal surgery, ulcer site, operation type, shock on admission, postoperative general complications,and intra-abdominal and/or wound infections), serum analyses and radiological findings.RESULTS: The overall mortality rate was 4.0%. Among all factors, an age above 65 years, one or more associated diseases, delayed abdominal surgery, shock on admission,postoperative abdominal complications and/or wound infections, were significantly associated (χ2) with increased mortality in patients undergoing surgery (0.0001<P<0.03).CONCLUSION: Factors such as concomitant diseases, shock on admission, delayed surgery, and postoperative abdominal and wound infections are significantly associated with fatal outcomes and need careful evaluation within the general workup of patients admitted for perforated peptic ulcer.

  8. Significant Factors Influencing Rural Residents’ Well-Being with Regard to Electricity Consumption: An Empirical Analysis in China

    Directory of Open Access Journals (Sweden)

    Sen Guo

    2016-11-01

    Full Text Available The electric universal service policy, which has been implemented for many years in China, aims to meet the basic electricity demands of rural residents. Electricity consumption can facilitate the daily life of rural residents, such as lighting and cooking, which are necessary to their well-being. In practice, the well-being of rural residents due to electricity consumption is influenced by many factors. Therefore, to improve the well-being of rural residents, it is quite necessary to identify and optimize the significant factors that make the electric universal service policy play its prescribed role as well as possible. In this paper, the significant factors influencing rural residents’ well-being obtained from electricity consumption were identified and discussed by employing the Ordered Probit model. The results indicate that: (1 there are six significant factors, of which ‘educational level’, ‘health condition’, ‘each person income of a family per month’, and ‘service time of household appliances’ play positive roles in rural residents’ well-being, while ‘average power interruption times’ and ‘monthly electric charges’ have negative impacts; (2 for significant factors with positive roles, ‘educational level’ and ‘health condition’ show larger marginal effects on rural residents’ well-being; and (3 for significant factors with negative impacts, ‘average power interruption times’ has the greatest marginal effect. Finally, policy implications are proposed for improving rural residents’ well-being, which can also contribute to the effective implementation of the electric universal service policy in China.

  9. Significant gender difference in serum levels of fibroblast growth factor 21 in Danish children and adolescents

    DEFF Research Database (Denmark)

    Bisgaard, Amalie; Sørensen, Kaspar; Johannsen, Trine Holm

    2014-01-01

    INTRODUCTION: Fibroblast Growth Factor 21 (FGF21) is a novel metabolic factor with effect on glucose and lipid metabolism, and shown to be elevated in diseases related to metabolic syndrome. Due to the increasing frequency of metabolic syndrome in the pediatric population, and as FGF21 studies...... in children are limited, we investigated baseline serum levels of FGF21 in healthy children during an oral glucose tolerance test. METHODS: A total of 179 children and adolescents from the COPENHAGEN Puberty Study were included. An OGTT with glucose and insulin measurements, a dual energy X-ray absorptiometry...... (DXA) scan and a clinical examination including pubertal staging were done on all subjects. Serum levels of FGF21, adiponectin, and leptin were determined by immunoassays at baseline. RESULTS: The girls had significantly higher levels of FGF21 compared with boys (155 pg/mL vs. 105 pg/mL, P = 0.04). 38...

  10. Expression and clinical significance of connective tissue growth factor (CTGF) in Graves' ophthalmopathy.

    Science.gov (United States)

    Huang, Yi-Ming; Chang, Pei-Chen; Wu, Shi-Bei; Kau, Hui-Chuan; Tsai, Chieh-Chih; Liu, Catherine Jui-Ling; Wei, Yau-Huei

    2017-05-01

    To examine the expression of connective tissue growth factor (CTGF) in human cultured orbital fibroblasts from patients with Graves' ophthalmopathy (GO) and investigate whether a correlation exists between the presence of CTGF protein and clinical parameters of the disease. The protein expression levels of CTGF were analysed by western blots in cultured orbital fibroblasts from 10 patients with GO and 7 age-matched normal controls. Associations between the protein expression of CTGF and the clinical factors of GO, including clinical demographics, thyroid function, clinical activity score (CAS) and ophthalmopathy index (OI), was evaluated. The mean protein expression levels of CTGF in the GO orbital fibroblasts were significantly higher than those of normal controls (ppathophysiology of GO. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  11. Socio-demographic factors and self-reported funtional status: the significance of social support

    Directory of Open Access Journals (Sweden)

    Vlachonikolis IG

    2002-10-01

    Full Text Available Abstract Background The aim of the present work was to investigate the relative importance of socio-demographic and physical health status factors for subjective functioning, as well as to examine the role of social support. Methods A cross-sectional health survey was carried out in a Greek municipality. 1356 adults of the general population were included in the study. Personal interviews were conducted with house-to-house visits. The response rate was 91.2%. Functioning has been measured by five indexes: 'The Social Roles and Mobility' scale (SORM, 'The Self-Care Restrictions' scale (SCR, 'The Serious Limitations' scale (SL, 'The Minor Self-care Limitations' scale (MSCR and 'The Minor Limitations in Social Roles and Mobility' scale (MSORM. Results Among the two sets of independent variables, the socio-demographic ones had significant influence on the functional status, except for MSORM. Allowing for these variables, the physical health status indicators had also significant effects on all functioning scales. Living arrangements and marital status had significant effects on four out of five indexes, while arthritis, Parkinson's disease, past stroke and kidney stones had significant effects on the SCR and SL scales. Conclusions These results suggest that socio-demographic factors are as important as physical health variables in affecting a person's ability to function normally in their everyday life. Social support appears to play a significant role in explaining differences in subjective functioning: people living alone or only with the spouse, particularly the elderly, seem to be in greater risk for disability problems and should be targeted by preventive programs in the community.

  12. Prevalence and risk factors for clinically significant upper gastrointestinal bleeding in patients with severe acute pancreatitis.

    Science.gov (United States)

    Zhan, Xian Bao; Guo, Xiao Rong; Yang, Jing; Li, Jie; Li, Zhao Shen

    2015-01-01

    To investigate the prevalence and risk factors of upper gastrointestinal bleeding (UGIB) in patients with severe acute pancreatitis (SAP). Altogether 101 patients were admitted to the Department of Gastroenterology, Changhai Hospital, Second Medical Military Hospital from July 2006 to June 2010 due to SAP. Their prevalence and risk factors of UGIB were retrospectively analyzed. In total, 18 (17.8%) patients developed UGIB and 13 received endoscopic examination, which yielded six cases of acute gastric mucosal lesions (AGML), five of peptic ulcers (PU) and two of pancreatic necrotic tissue invading the duodenal bulb and presenting as multilesion, honeycomb-like ulcer. The mortality rate of UGIB patients was much higher than that of non-UGIB patients (44.4% vs 10.8%, P = 0.0021). Univariate analysis revealed that the risk factors for UGIB included the Acute Physiology and Chronic Health Evaluation II (APACHE II) score, computed tomography severity index (CTSI), Ranson score, arterial blood pH and PaO2 , serum blood urea nitrogen and creatinine concentrations, platelet count, shock, sepsis and organ failure, mechanical ventilation, heparinized continuous renal replacement therapy and total parenteral nutrition. Multivariate logistic regression revealed that APACHE II score and CTSI were significant risk factors while PaO2 was the protective factor for UGIB in SAP. UGIB is a common complication with poor prognosis due mainly to PU and AGML. Patients having SAP with high APACHE II scores and CTSI or low PaO2 should be considered to be at high risk for UGIB. © 2014 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.

  13. Mycalamide A Shows Cytotoxic Properties and Prevents EGF-Induced Neoplastic Transformation through Inhibition of Nuclear Factors

    Science.gov (United States)

    Dyshlovoy, Sergey A.; Fedorov, Sergey N.; Kalinovsky, Anatoly I.; Shubina, Larisa K.; Bokemeyer, Carsten; Stonik, Valentin A.; Honecker, Friedemann

    2012-01-01

    Mycalamide A, a marine natural compound previously isolated from sponges, is known as a protein synthesis inhibitor with potent antitumor activity. However, the ability of this compound to prevent malignant transformation of cells has never been examined before. Here, for the first time, we report the isolation of mycalamide A from ascidian Polysincraton sp. as well as investigation of its cancer preventive properties. In murine JB6 Cl41 P+ cells, mycalamide A inhibited epidermal growth factor (EGF)-induced neoplastic transformation, and induced apoptosis at subnanomolar or nanomolar concentrations. The compound inhibited transcriptional activity of the oncogenic nuclear factors AP-1 and NF-κB, a potential mechanism of its cancer preventive properties. Induction of phosphorylation of the kinases MAPK p38, JNK, and ERK was also observed at high concentrations of mycalamide A. The drug shows promising potential for both cancer-prevention and cytotoxic therapy and should be further developed. PMID:22822368

  14. Recidivism in female offenders: PCL-R lifestyle factor and VRAG show predictive validity in a German sample.

    Science.gov (United States)

    Eisenbarth, Hedwig; Osterheider, Michael; Nedopil, Norbert; Stadtland, Cornelis

    2012-01-01

    A clear and structured approach to evidence-based and gender-specific risk assessment of violence in female offenders is high on political and mental health agendas. However, most data on the factors involved in risk-assessment instruments are based on data of male offenders. The aim of the present study was to validate the use of the Psychopathy Checklist Revised (PCL-R), the HCR-20 and the Violence Risk Appraisal Guide (VRAG) for the prediction of recidivism in German female offenders. This study is part of the Munich Prognosis Project (MPP). It focuses on a subsample of female delinquents (n = 80) who had been referred for forensic-psychiatric evaluation prior to sentencing. The mean time at risk was 8 years (SD = 5 years; range: 1-18 years). During this time, 31% (n = 25) of the female offenders were reconvicted, 5% (n = 4) for violent and 26% (n = 21) for non-violent re-offenses. The predictive validity of the PCL-R for general recidivism was calculated. Analysis with receiver-operating characteristics revealed that the PCL-R total score, the PCL-R antisocial lifestyle factor, the PCL-R lifestyle factor and the PCL-R impulsive and irresponsible behavioral style factor had a moderate predictive validity for general recidivism (area under the curve, AUC = 0.66, p = 0.02). The VRAG has also demonstrated predictive validity (AUC = 0.72, p = 0.02), whereas the HCR-20 showed no predictive validity. These results appear to provide the first evidence that the PCL-R total score and the antisocial lifestyle factor are predictive for general female recidivism, as has been shown consistently for male recidivists. The implications of these findings for crime prevention, prognosis in women, and future research are discussed.

  15. EXPRESSION AND SIGNIFICANCE OF BASIC FIBROBLAST GWOWTH FACTOR AND FIBROBLAST GROWTH FACTOR RECEPTOR-1 IN OVARIAN EPITHELIAL NEOPLASM

    Institute of Scientific and Technical Information of China (English)

    高尚风; 杨蓉; 高博; 刘惠喜

    2003-01-01

    fibroblast growth factor (bFGF), fibroblast growth factor receptor-1 (FGFR-1) and carcinogenesis and progression of ovarian epithelial neoplasm. Methods Ten cases of normal ovarian tissues and 75 cases of ovarian epithelial neoplasm tissues were detected by immunohistochemical methods: S-P for bFGF, FGFR-1,double immunohistochemistry Lab-SA for Ki-67 antigen and bFGF. Results The expression level of bFGF, FGFR-1in ovarian epithelium and ovarian epithelial neoplasm showed a step-wise increase in the following order:normal〈benign〈borderline〈malignant; The expression level and intensity of bFGF and FGFR-1 were increased with the decrease of differentiation degree and increase of clinical stage in ovarian carcinoma; There was no statistical difference between the expression of bFGF, FGFR-1 in serous cystadenocarcinoma and that of mucinous cystadenocarcinoma; The expression of bFGF was correlated with that of FGFR-1 in neoplastic tissues; There were positive expression rates of bFGF and Ki-67 antigen in ovarian epithelial neoplasm. Conclusion As an important proliferative factor, bFGF plays an important role in carcinogenisis and progression of ovarian epithelial neoplasm.

  16. Factors controlling reservoir quality in tertiary sandstones and their significance to geopressured geothermal production

    Energy Technology Data Exchange (ETDEWEB)

    Loucks, R.G.; Richmann, D.L.; Milliken, K.L.

    1981-01-01

    Variable intensity of diagenesis is the factor primarily responsible for contrasting regional reservoir quality of Tertiary sandstones from the upper and lower Texas coast. Detailed comparison of Frio sandstone from the Chocolate Bayou/Danbury Dome area, Brazoria County, and Vicksburg sandstones from the McAllen Ranch Field area, Hidalgo County, reveals that extent of diagenetic modification is most strongly influenced by (1) detrital mineralogy and (2) regional geothermal gradients. The regional reservoir quality of Frio sandstones from Brazoria County is far better than that characterizing Vicksburg sandstones from Hidalgo County, especially at depths suitable for geopressured geothermal energy production. However, in predicting reservoir quality on a site-specific basis, locally variable factors such as relative proportions for porosity types, pore geometry as related to permeability, and local depositional environment must also be considered. Even in an area of regionally favorable reservoir quality, such local factors can significantly affect reservoir quality and, hence, the geothermal production potential of a specific sandstone unit.

  17. Human papillomavirus shows highly variable prevalence in esophageal squamous cell carcinoma and no significant correlation to p16INK4a overexpression

    DEFF Research Database (Denmark)

    Michaelsen, Sanne Høxbroe; Larsen, Christian Grønhøj; von Buchwald, Christian

    2014-01-01

    INTRODUCTION: This review investigates the role of p16(INK4a) as a marker of transcriptionally active human papillomavirus (HPV) in esophageal squamous cell carcinoma (ESCC) and the regional prevalence of HPV in ESCC. METHODS: PubMed, EMBASE, and the Cochrane Library were systematically searched...... countries. HPV DNA was detected in 12.0% (n = 161) of 1347 specimens, and p16(INK4a) was detected in 33.9% (n = 209) of 617 specimens. The HPV presence varied from 0% to 70% among the studies. The prevalence of p16(INK4a) overexpression in HPV-positive and HPV-negative specimens demonstrated...... no statistically significant difference, neither for the combined data (p = 0.7507) nor for any individual study, and detection of p16(INK4a) overexpression did not affect the odds of tumors being HPV positive (odds ratio = 1.0666 with 95% confidence interval 0.7040-1.6157). In a pooled analysis, the sensitivity...

  18. Change and significance of nuclear factor-κB in adriamycin induced cardiomyopathy in rats

    Institute of Scientific and Technical Information of China (English)

    LI Hong-li; LIU Bin; ZHOU Ling-wang; YU Wei-han

    2005-01-01

    Background This study aimed at investigating the change and significance of nuclear factor-κB (NF-κB) in cardiomyopathy induced by adriamycin (ADR) in rats.Methods Sixty male Wistar rats were randomly divided into three groups: control, ADR and ADR+pyrrolidine dithiocarbamate (PDTC) groups. After 30-day experiment, myocardial histopathological observation was performed. Location and distribution of NF-κB p50 was examined by immunohistochemical assay. Expression of NF-κB p50 protein was examined by immunobolt assay. Electrophoretic Mobility Shift Assay examined activity of NF-κB; Myocardium p53 gene expression was examined by RT-PCR analysis. Results The myocardial lesions of rats were less pronounced in ADR +PDTC group than in ADR group. Compared with control group, there were many myocardium nucleuses, which expressed NF-κB p50 and distribute under epicardium. Expression of NF-κB p50 protein in nucleus increased significantly in ADR group. The NF-κB binding activity increased significantly in ADR group. Myocardium expressions of p53 mRNA increased in ADR group. Conclusions The NF-κB binding activity increased significantly in cardiomyopathy induced by ADR in rats. Moreover, NF-κB plays an important role in causing degeneration of myocardial tissue and regulating expression of related-apoptosis genes.

  19. The Brazos River (Texas) Sequence Shows Significant Cooling in the Waning Stages of the Tsunami Surges Caused by the Chicxulub Impact

    Science.gov (United States)

    Smit, J.; Vellekoop, J.

    2013-05-01

    The Brazos river K-Pg sequences are among the best preserved and studied in the world, yet any interpretation remains highly controversial. Most researchers, however, agree that the coarse clastic deposits are the direct result of a train of Chicxulub impact triggered tsunami surges. Alternative interpretations such as low stand deposits or (super) storm deposits lack sedimentological support. The entire impact related deposit starts with a strong ground shaking from the impact-induced earthquake, disintegrating unconsolidated uppermost Maastrichtian muds, and opening 0.5m deep and 5 m long fissures filled with spherule-rich debris. The disintegrated debris has been taken up in a coarse mass-flow, just underlying the first coarse tsunami deposit containing impact spherules from Chicxulub. One to four distinct tsunami surges follow the basal surge, each leaving a graded coarse to medium sand deposit assembled from coarse debris strewn on the local seafloor such as glauconitic pellets, fish-debris and near coastal foraminifers. The medium-grained sand layers are typically cross-bedded in linguoid and linguoid-climbing current-ripple sets, indicating a dominant S to SE seaward directed flow. Such climbing ripple-sets are found in most tsunami deposits in NE Mexico. These climbing ripples indicate an extremely high suspension load, quickly settling on the seafloor in the waning tsunami surge backflow-currents. Occasionally, the climbing ripple directions are reversed, showing the upflow direction of the incoming tsunami surge. Such linguoid climbing ripple sets have often been mistaken for storm-wave induced hummocky cross-bedding (HCS), leading to storm-deposit hypotheses. The final phase of settling out of the tsunami surges, may be re-suspension due to impact-triggered storms in the Gulf, is represented by continuously graded, very fine-grained sand to silt deposit. This is initially a hard 10 cm thick silty lime-mudstone layer with plant debris, grading into drab

  20. The prognostic significance of fibroblast growth factor receptor 4 in non-small-cell lung cancer

    Directory of Open Access Journals (Sweden)

    Huang H

    2015-05-01

    Full Text Available Hong-ping Huang, Hui Feng, Hong-bo Qiao, Ze-xiang Ren, Ge-dong ZhuDepartment of General Medicine, Linyi Hospital Affiliated to Shandong University, Linyi City, People’s Republic of China Background: Fibroblast growth factor receptor 4 (FGFR4 has been proved to be correlated with progression and prognosis in many cancers. However, the significance of FGFR4 in non-small-cell lung cancer (NSCLC is still not well elucidated.Methods: In our experiment, we detected FGFR4 expression in 237 samples of NSCLC with immunohistochemistry, and further analyzed the correlation between FGFR4 and clinicopathologic features of NSCLC with chi-square test. Moreover, we evaluated the prognostic value of FGFR4 by Kaplan–Meier survival curve and Cox regression model. By regulating the expression of FGFR4 by overexpression or knockdown, we assessed the role of FGFR4 on NSCLC cell proliferation.Results: FGFR4 expression was high in NSCLC (46.8%, 111/237. FGFR4 expression was significantly associated with tumor diameter (P=0.039. With univariate (P=0.009 and multivariate (P=0.002 analysis, FGFR4 was identified as an independent prognostic factor in NSCLC (P=0.009. Moreover, FGFR4 can promote the proliferation of NSCLC cell lines.Conclusion: FGFR4 is an independent prognostic biomarker in NSCLC. FGFR4 can accelerate the proliferation of NSCLC cell lines, indicating FGFR4 could be a potential drug target of NSCLC.Keywords: fibroblast growth factor 4, non-small-cell lung cancer, prognosis, proliferation

  1. A Newly Emergent Turkey Arthritis Reovirus Shows Dominant Enteric Tropism and Induces Significantly Elevated Innate Antiviral and T Helper-1 Cytokine Responses.

    Directory of Open Access Journals (Sweden)

    Tamer A Sharafeldin

    Full Text Available Newly emergent turkey arthritis reoviruses (TARV were isolated from tendons of lame 15-week-old tom turkeys that occasionally had ruptured leg tendons. Experimentally, these TARVs induced remarkable tenosynovitis in gastrocnemius tendons of turkey poults. The current study aimed to characterize the location and the extent of virus replication as well as the cytokine response induced by TARV during the first two weeks of infection. One-week-old male turkeys were inoculated orally with TARV (O'Neil strain. Copy numbers of viral genes were estimated in intestines, internal organs and tendons at ½, 1, 2, 3, 4, 7, 14 days Post inoculation (dpi. Cytokine profile was measured in intestines, spleen and leg tendons at 0, 4, 7 and 14 dpi. Viral copy number peaked in jejunum, cecum and bursa of Fabricius at 4 dpi. Copy numbers increased dramatically in leg tendons at 7 and 14 dpi while minimal copies were detected in internal organs and blood during the same period. Virus was detected in cloacal swabs at 1-2 dpi, and peaked at 14 dpi indicating enterotropism of the virus and its early shedding in feces. Elevation of IFN-α and IFN-β was observed in intestines at 7 dpi as well as a prominent T helper-1 response (IFN-γ at 7 and 14 dpi. IFN-γ and IL-6 were elevated in gastrocnemius tendons at 14 dpi. Elevation of antiviral cytokines in intestines occurred at 7dpi when a significant decline of viral replication in intestines was observed. T helper-1 response in intestines and leg tendons was the dominant T-helper response. These results suggest the possible correlation between viral replication and cytokine response in early infection of TARV in turkeys. Our findings provide novel insights which help elucidate viral pathogenesis in turkey tendons infected with TARV.

  2. Which oropharyngeal factors are significant risk factors for obstructive sleep apnea? An age-matched study and dentist perspectives

    Directory of Open Access Journals (Sweden)

    Ruangsri S

    2016-07-01

    , narrow lateral pharyngeal wall, and Mallampati class 4. There were two other significant factors associated with having OSA, namely, BMI and Mallampati classification. The adjusted odds ratios (95% confidence interval of these two factors were 1.445 (1.017, 2.052 and 5.040 (1.655, 15.358, respectively. Conclusion: Dentists may play an important role in the detection of OSA in patients with high BMI through careful oropharyngeal examination in routine dental treatment. A large torus mandibularis, Mallampati class 4, and a narrow lateral pharyngeal wall are important anatomical risk factors for OSA. Keywords: obstructive sleep apnea, neck circumference, torus mandibularis, tongue size, ­Mallampati classification, palatal vault, lateral pharyngeal wall, hypertension

  3. Clinically Significant Fatigue: Prevalence and Associated Factors in an International Sample of Adults with Multiple Sclerosis Recruited via the Internet

    OpenAIRE

    Weiland, Tracey J.; Jelinek, George A.; Claudia H. Marck; Emily J Hadgkiss; van der Meer, Dania M.; Pereira, Naresh G.; Taylor, Keryn L.

    2015-01-01

    Background Fatigue contributes a significant burden of disease for people with multiple sclerosis (PwMS). Modifiable lifestyle factors have been recognized as having a role in a range of morbidity outcomes in PwMS. There is significant potential to prevent and treat fatigue in PwMS by addressing modifiable risk factors. Objectives To explore the associations between clinically significant fatigue and demographic factors, clinical factors (health-related quality of life, disability and relapse...

  4. Expression and significance of PTEN, hypoxia-inducible factor-1 alpha in colorectal adenoma and adenocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Ying-An Jiang; Li-Fang Fan; Chong-Qing Jiang; You-Yuan Zhang; He-Sheng Luo; Zhi-Jiao Tang; Dong Xia; Ming Wang

    2003-01-01

    AIM: To investigate the expression and significance of PTEN,hypoxia-inducible factor-1 alpha (HIF-1α), and targeting gene VEGF during colorectal carciogenesis.METHODS: Total 71 cases colorectal neoplasms (9 cases of colorectal adenoma and 62 colorectal adenocarcinoma)were formalin fixed and paraffin-embedded, and all specimens were evaluated for PTEN mRNA, HIF-1α mRNA and VEGF protein expression. PTEN mRNA, HIF-1α mRNA were detected by in situ hybridization. VEGF protein was identified by citrate-microwave SP immunohistochemical method.RESULTS: There were significant differences in PTEN, HIF1α and VEGF expression between colorectal adenomas and colorectal adenocarcinoma (P<0.05). The level of PTEN expression decreased as the pathologic stage increased.Conversely, HIF-1α and VEGF expression increased with the Dukes stage as follows: stage A (0.1029±0.0457:0.1207± 0.0436), stage B (0.1656±0.0329: 0.1572±0.0514),and stage C+D (0.2335±0.0748: 0.2219±0.0803). For PTEN expression, there was a significant difference among Dukes stage A, B, and C+D, and the level of PTEN expression was found to be significant higher in Dukes stage A or B than that of Dukes stage C or D. For HIF-1α expression,there was a significant difference between Dukes stage A and B, and the level of HIF-1α expression was found to be significantly higher in Dukes stage C+D than that of Dukes stage A or B. The VEGF expression had similar results as HIF-1α expression. In colorectal adenocarcinoma,decreased levels of PTEN were significantly associated with increased expression of HIF-1α mRNA (r=-0.36, P<0.05)and VEGF protein (r=-0.48, P<0.05) respectively. The levels of HIF-1 were positively correlated with VEGF expression (r=0.71, P<0.01).CONCLUSION: Loss of PTEN expression and increased levels of HIF-1α and VEGF may play an important role in carcinogenesis and progression of colorectal adenocarcinoma.

  5. EFFECT SIGNIFICANCE ASSESSMENT OF THE THERMODYNAMICAL FACTORS ON THE SOLID OXIDE FUEL CELL OPERATION

    Directory of Open Access Journals (Sweden)

    V. A. Sednin

    2015-01-01

    Full Text Available Technologies of direct conversion of the fuel energy into electrical power are an upcoming trend in power economy. Over the last decades a number of countries have created industrial prototypes of power plants on fuel elements (cells, while fuel cells themselves became a commercial product on the world energy market. High electrical efficiency of the fuel cells allows predictting their further spread as part of hybrid installations jointly with gas and steam turbines which specifically enables achieving the electrical efficiency greater than 70 %. Nevertheless, investigations in the area of increasing efficiency and reliability of the fuel cells continue. Inter alia, research into the effects of oxidizing reaction thermodynamic parameters, fuel composition and oxidation reaction products on effectiveness of the solid oxide fuel cells (SOFC is of specific scientific interest. The article presents a concise analysis of the fuel type effects on the SOFC efficiency. Based on the open publications experimental data and the data of numerical model studies, the authors adduce results of the statistical analysis of the SOFC thermodynamic parameters effect on the effectiveness of its functioning as well as of the reciprocative factors of these parameters and gas composition at the inlet and at the outlet of the cell. The presented diagrams reflect dimension of the indicated parameters on the SOFC operation effectiveness. The significance levels of the above listed factors are ascertained. Statistical analysis of the effects of the SOFC functionning process thermodynamical, consumption and concentration parameters demonstrates quintessential influence of the reciprocative factors (temperature – flow-rate and pressure – flow-rate and the nitrogen N2 and oxygen O2 concentrations on the operation efficiency in the researched range of its functioning. These are the parameters to be considered on a first-priority basis while developing mathematical models

  6. Sequential changes of hypoxia- inducible factor 1 alpha in experimental spinal cord injury and its significance

    Institute of Scientific and Technical Information of China (English)

    鞠延; 贺民; 毛伯镛

    2002-01-01

    Objective: To study the sequential changes of HIF-1α(hypoxia-inducible factor 1 alpha) in experimental spinalcord injury in rats and to analyze its potential effects inSCI.Methods: A static compression model of SCI wasemployed in this study. Expressions of HIF-1α weremeasured with immunohistochemical staining, while flowcytometry was used to determine the apoptotic ratio andbcl-2 expressions.Results: HIF-1α began to increase 1 day after injury,and reached the peak at 3-7 days. Two weeks later, itdeclined significantly. The sequential changes of HIF-1αcoincided well with the alterations of apoptotic ratio andcontents of bel-2.Conclusions: HIF-1α possibly participates in thesecondary ischemic and hypoxic procedures after spinalcord injury, and may mediate the traumatic apoptosis.Further understanding of HIF-1α may provide newtherapeutic regimens for SCI.

  7. [Microbiological colonisation of bovine teat canal--significance and influencing factors].

    Science.gov (United States)

    Paduch, Jan-Hendrik; Krömker, Volker

    2011-01-01

    The teat canal of lactating dairy cattle is of particular importance for the defence of facultative pathogenic and pathogenic microorganisms (e.g., staphylococci) invading the bovine udder. Furthermore the canal is usually colonized with microorganisms, too. In addition to microorganisms inhibiting mastitis pathogens the teat canal is colonized by staphylococci. The microbial colonization can be influenced by the environment of the animals, the care and disinfection of the teat skin and indirectly by the effects of forces being associated with machine milking. Because of vacuum fluctuations occurring under the teat tip microorganisms, which colonize the teat canal, can invade the bovine mammary gland and cause infections there. This paper gives a review of the microbial colonization of the bovine teat duct and of influencing factors on the microbial populations as well as of the significance of the teat canal colonization for the development of mastitis.

  8. Physiological knock-knee in preschool children: prevalence, correlating factors, gait analysis, and clinical significance.

    Science.gov (United States)

    Lin, C J; Lin, S C; Huang, W; Ho, C S; Chou, Y L

    1999-01-01

    Physiological knock-knee (PKK) was categorized by measuring intermalleolar distance (IMD), a clinically simple method, to evaluate the prevalence and correlating factors in 305 preschool children. The prevalence in this cross-sectional study was relatively high, and it was age related (p = 0.002; 64, 44, and 34% for ages 3-4, 4-5, and 5-6 years, respectively). The following factors were correlated with PKK: use of walking chair early (p = 0.0001), independently walked late (p = 0.0005), dependently walked longer (p = 0.0001), concurrence with flatfoot (p = 0.001), and angular deformity (toe in/out, p = 0.03). Gait analysis, with spatiotemporal, kinematics, and kinetics parameters, was performed to evaluate the ambulatory significance. Preschool children with PKK have a shorter stride length (p = 0.02) and a slower walking speed (p = 0.004). Dynamic hyperextension of the knee is noted for 8 degrees during the whole gait cycle (p PKK is a variable that should be considered in the development of mature gait for preschool children.

  9. Treatment of angular cheilitis. The significance of microbial analysis, antimicrobial treatment, and interfering factors.

    Science.gov (United States)

    Ohman, S C; Jontell, M

    1988-10-01

    This prospective study evaluated the significance of microbial analysis and antimicrobial treatment for the cure of angular cheilitis. Furthermore, various etiologic factors were investigated for their relative effect on the healing process. The study included 1) an open trial with 50 patients infected by Candida albicans and/or Staphylococcus aureus, and 2) an intraindividual comparison of eight patients with bilateral lesions infected by Candida albicans as the only detected pathogen. After a base-line examination the patients received ointments containing nystatin and/or fusidic acid, on the basis of the outcome of an initial microbial analysis. The patients were evaluated clinically, photographed, and examined for microorganisms at different time intervals. Ninety-six per cent of the patients who participated in the open trial had no sign of infection after 42 days of treatment. Lesions in the double-blind study, treated with nystatin, were healed after 28 days, whereas lesions that received placebo persisted throughout the treatment period. Increasing age, dry skin, and extended skinfolds at the corner of the mouth were factors closely related to the length of the healing process.

  10. Expression of transcription factor Pokemon in non-small cell lung cancer and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    ZHAO Zhi-hong; WANG Sheng-fa; YU Liang; WANG Ju; CHANG Hao; YAN Wei-li; FU Kai; ZHANG Jian

    2008-01-01

    Background Transcription factor Pokemon,a central regulation gene of the important tumor suppressor ARF gene,exerted its activity by acting upstream of many tumor-suppressing genes and proto-oncogenes.Its expression in non-small cell lung cancer (NSCLC)and its clinical significance remains unclear.The aim of this study was to investigate the expression of Pokemon in NSCLC and to explore its correlation with the clinical pathological characteristics and its influence on patients'prognosis.Methods Fifty-five cases of NSCLC were involved in this study.The expression of Pokemon in the tumor tissue,the corresponding tumor adjacent tissue and the surrounding tissue was detected via reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting,with the aim of investigating the correlation between the expression of Pokemon in tumor tissue of NSCLC and its clinicaI pathological characteristics.Moreover,a prognostic analysis was carried out based upon the immunohistochemical(IHC)detection of the expression of Pokemon gene in archival tumor specimens (5 years ago) of 62 cases of NSCLC.Results Statistical significance of the expression of Pokemon mRNA and protein was determined in the tumor tissue,the tumor adjacent tissue and the surrounding tissue (P<0.05).The expression of Pokemon was determined not to be associated with the patients'sex,age,smoking condition,tumor differentiation degree,histology and lymph node metastasis condition.However,its relationship with TNM staging was established(P<0.05).Furthermore,it was shown that the suwival rate of patients with negative Pokemon expression was significantly higher than that of those with positive Pokemon expression(P=0.004),therefore,the expression of Pokemon is believed to be an independent factor affectinq prognosis (P=0.034).Concluaion Pokemon was over-expressed in NSCLC tissue and the expression of Pokemon might be of clinical significance in non-small cell lung cancer prognostic evaluation.

  11. DEVELOPMENT OF QUESTIONNAIRE FOR MEASURING FACTORS OF SIGNIFICANCE OF AN ELDERLY PERSON AS ANOTHER FOR THE YOUNGER GENERATION

    Directory of Open Access Journals (Sweden)

    Natalia V. KHALINA

    2015-01-01

    Full Text Available Social and political processes of the present time, actualize in the minds of the younger generation of Russians importance for the development of the country. But it would be wrong to assume no significant experience in the elderly and to consider identity apart from family ties. Relevance to this key acts the process of intergenerational transmission, when the younger generation can assimilate the experience of close, meaningful elderly. The paper drew attention to the dedicated A.V. Petrovsky importance of interpersonal factors: credibility (reference, attraction (emotional state, institutionalized role (power status. On the basis of the three-factor model proposed by the author's questionnaire for measuring factors like the importance of an elderly as a significant Another for the younger generation. The authors conducted a tested methodology for determining the significance of an old man for youth. The statistical analysis survey was conducted using a Rasch model, which is an important feature of the subjective objectivity of assessments. It shows the process of correction of the questionnaire based on the theory of measurement of latent variables. Designed questionnaire contains twenty-four judgments, respondents were asked to express the degree of agreement with these judgments in four grades. Thus, twenty-four indicator variables actually measure the latent variable "importance of the elderly as a significant Another for the younger generation", and are compatible with each other, enabling them to measure the factors, the importance of the elderly as a significant Another for the younger generation: the authority of the (reference, attraction (emotional status institutionalized the role of (status of power.

  12. Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis

    Institute of Scientific and Technical Information of China (English)

    Rong-Li Piao; David R Brigstock; Jie Zhu; Man-Li Zhang; Run-Ping Gao

    2012-01-01

    AIM:To determine the utility of connective tissue growth factor (CCN2/CTGF) for assessing hepatic fibrosis in hepatitis B virus (HBV)-induced chronic liver diseases (CLD-B).METHODS:Enzyme-linked immunosorbent assay was used to measure CCN2 in sera from 107 patients with chronic hepatitis B (CHB) and 39 patients with HBVinduced active liver cirrhosis and 30 healthy individuals.Liver samples from 31 patients with CHB,8 patients with HBV-induced liver cirrhosis and 8 HBV carriers with normal liver histology were examined for transforming growth factor β-1 (TGF-β1) or CCN2 mRNA levels by in situ hybridization,and computer image analysis was performed to measure integrated optimal density (IOD) of CCN2 mRNA-positive cells in liver tissues.Histological inflammation grading and fibrosis staging were evaluated by H and E staining and Van Gieson's method.RESULTS:Serum CCN2 concentrations were,respectively,4.0-or 4.9-fold higher in patients with CHB or active liver cirrhosis as compared to healthy individuals (P < 0.01).There was good consistency between the levels of CCN2 in sera and CCN2 mRNA expression in liver tissues (r =0.87,P < 0.01).The levels of CCN2 in sera were increased with the enhancement of histological fibrosis staging in patients with CLD-B (r =0.85,P < 0.01).Serum CCN2 was a reliable marker for the assessment of liver fibrosis,with areas under the receiver operating characteristic (ROC) curves (AUC) of 0.94 or 0.85 for,respectively,distinguishing normal liver controls from patients with F1 stage liver fibrosis or discriminating between mild and significant fibrosis.CONCLUSION:Detection of serum CCN2 in patients with CLD-B may have clinical significance for assessment of severity of hepatic fibrosis.

  13. Epidermal growth factor receptor gene amplification and protein expression in glioblastoma multiforme: prognostic significance and relationship to other prognostic factors.

    Science.gov (United States)

    Layfield, Lester J; Willmore, Carlynn; Tripp, Sheryl; Jones, Claudia; Jensen, Randy L

    2006-03-01

    Epidermal growth factor receptor (EGFR) overexpression occurs in a significant percentage of cases of glioblastoma multiforme (GBM), and amplification has been found in approximately 40% of these neoplasms. Controversy exists as to the prognostic significance of EGFR gene amplification: some reports have indicated that amplification is associated with a poor prognosis, while other authors have reported no relationship between gene amplification and prognosis. Some reports have found a poor prognosis to be associated with amplification of the EGFR gene in patients of all ages with GBM, while other authors have found EGFR amplification to be an independent predictor of prolonged survival in patients with GBM who are older than 60 years of age. The authors studied a series of 34 specimens (32 patients) with histologically proven GBM by immunohistochemistry for the presence of EGFR overexpression and by fluorescence in situ hybridization (FISH) for gene amplification of the EGFR gene. Results of these studies and data on patient age, sex, functional status, therapy, and survival were correlated to determine which variables were predictive of survival. p53 expression was also determined by immunohistochemistry and correlated with the other variables and survival.

  14. Detection and significance of serum inflammatory factors and oxidative stress indicators in diabetic retinopathy

    Institute of Scientific and Technical Information of China (English)

    Wei Gao; Jing Wang; Chao Zhang; Ping Qin

    2017-01-01

    Objective:To determine the serum inflammatory cytokines and oxidative stress parameters of diabetic retinopathy (DR) patients to explore their possible role in the DR.Methods: 116 cases of type 2 diabetic patients were selected from June 2015 to June 2016 in our hospital as research subjects, divided into diabetic Diabetes without retinopathy (NDR group,n = 63) and diabetic with retinopathy patients (DR group,n = 53). And 60 cases of healthy check-ups of the same period in our hospital medical center were selected as normal control group (NC). The VEGF, IL-6, TNF-α , MDA and SOD levels of three groups of patients were detected. Results:The IL-6 levels of NC group, NDR group and DR group were increased gradually, and the difference was statistically significant (P<0.05). The TNF-α levels of NC group, NDR group and DR group were increased gradually, and the difference was statistically significant (P<0.05). The VEGF levels of NC group, NDR group and DR group were increased gradually, and the difference was statistically significant (P<0.05). The malondialdehyde (MDA) levels of NC group, NDR group and DR group increased gradually, and the difference was statistically significant (P<0.05). The superoxide dismutase (SOD) levels of NC group, NDR group and DR group were decreased gradually, and the difference was statistically significant (P<0.05). Conclusions: DR patients express high levels of IL-6, TNF-α and VEGF, and there exists significant oxidative stress in DR, which shows that the inflammation occurrence and oxidative stress state play an important role in the development of DR.

  15. Factors correlating with significant differences between X-ray structures of myoglobin

    Energy Technology Data Exchange (ETDEWEB)

    Rashin, Alexander A., E-mail: alexander-rashin@hotmail.com [BioChemComp Inc., 543 Sagamore Avenue, Teaneck, NJ 07666 (United States); Iowa State University, 112 Office and Lab Bldg, Ames, IA 50011-3020 (United States); Domagalski, Marcin J. [University of Virginia, 1340 Jefferson Park Avenue, Jordan Hall, Room 4223, Charlottesville, VA 22908 (United States); Zimmermann, Michael T. [Iowa State University, 112 Office and Lab Bldg, Ames, IA 50011-3020 (United States); Minor, Wladek [University of Virginia, 1340 Jefferson Park Avenue, Jordan Hall, Room 4223, Charlottesville, VA 22908 (United States); Chruszcz, Maksymilian [University of Virginia, 1340 Jefferson Park Avenue, Jordan Hall, Room 4223, Charlottesville, VA 22908 (United States); University of South Carolina, 631 Sumter Street, Columbia, SC 29208 (United States); Jernigan, Robert L. [Iowa State University, 112 Office and Lab Bldg, Ames, IA 50011-3020 (United States); BioChemComp Inc., 543 Sagamore Avenue, Teaneck, NJ 07666 (United States)

    2014-02-01

    Conformational differences between myoglobin structures are studied. Most structural differences in whale myoglobin beyond the uncertainty threshold can be correlated with a few specific structural factors. There are always exceptions and a search for additional factors is needed. The results might have serious implications for biological insights from conformational differences. Validation of general ideas about the origins of conformational differences in proteins is critical in order to arrive at meaningful functional insights. Here, principal component analysis (PCA) and distance difference matrices are used to validate some such ideas about the conformational differences between 291 myoglobin structures from sperm whale, horse and pig. Almost all of the horse and pig structures form compact PCA clusters with only minor coordinate differences and outliers that are easily explained. The 222 whale structures form a few dense clusters with multiple outliers. A few whale outliers with a prominent distortion of the GH loop are very similar to the cluster of horse structures, which all have a similar GH-loop distortion apparently owing to intermolecular crystal lattice hydrogen bonds to the GH loop from residues near the distal histidine His64. The variations of the GH-loop coordinates in the whale structures are likely to be owing to the observed alternative intermolecular crystal lattice bond, with the change to the GH loop distorting bonds correlated with the binding of specific ‘unusual’ ligands. Such an alternative intermolecular bond is not observed in horse myoglobins, obliterating any correlation with the ligands. Intermolecular bonds do not usually cause significant coordinate differences and cannot be validated as their universal cause. Most of the native-like whale myoglobin structure outliers can be correlated with a few specific factors. However, these factors do not always lead to coordinate differences beyond the previously determined uncertainty

  16. Catchment process affecting drinking water quality, including the significance of rainfall events, using factor analysis and event mean concentrations.

    Science.gov (United States)

    Cinque, Kathy; Jayasuriya, Niranjali

    2010-12-01

    To ensure the protection of drinking water an understanding of the catchment processes which can affect water quality is important as it enables targeted catchment management actions to be implemented. In this study factor analysis (FA) and comparing event mean concentrations (EMCs) with baseline values were techniques used to asses the relationships between water quality parameters and linking those parameters to processes within an agricultural drinking water catchment. FA found that 55% of the variance in the water quality data could be explained by the first factor, which was dominated by parameters usually associated with erosion. Inclusion of pathogenic indicators in an additional FA showed that Enterococcus and Clostridium perfringens (C. perfringens) were also related to the erosion factor. Analysis of the EMCs found that most parameters were significantly higher during periods of rainfall runoff. This study shows that the most dominant processes in an agricultural catchment are surface runoff and erosion. It also shows that it is these processes which mobilise pathogenic indicators and are therefore most likely to influence the transport of pathogens. Catchment management efforts need to focus on reducing the effect of these processes on water quality.

  17. Prognostic significance of fibroblast growth factor receptor 4 polymorphisms on biochemical recurrence after radical prostatectomy in a Chinese population

    Science.gov (United States)

    Chen, Luyao; Lei, Zhengwei; Ma, Xin; Huang, Qingbo; Zhang, Xu; Zhang, Yong; Hao, Peng; Yang, Minggang; Zhao, Xuetao; Chen, Jun; Liu, Gongxue; Zheng, Tao

    2016-01-01

    Fibroblast growth factor receptor 4 (FGFR4) is a transmembrane receptor with ligand-induced tyrosine kinase activity and is involved in various biological and pathological processes. Several polymorphisms of FGFR4 are associated with the incidence and mortality of numerous cancers, including prostate cancer. In this study, we investigated whether the polymorphisms of FGFR4 influence the biochemical recurrence of prostate cancer in Chinese men after radical prostatectomy. Three common polymorphisms (rs1966265, rs2011077, and rs351855) of FGFR4 were genotyped from 346 patients with prostate cancer by using the Sequenom MassARRAY system. Kaplan–Meier curves and Cox proportional hazard models were used for survival analysis. Results showed biochemical recurrence (BCR) free survival was significantly affected by the genotypes of rs351855 but not influenced by rs1966265 and rs2011077. After adjusting for other variables in multivariable analysis, patients with rs351855 AA/AG genotypes showed significantly worse BCR-free survival than those with the GG genotype (HR = 1.873; 95% CI, 1.209–2.901; P = 0.005). Hence, FGFR4 rs351855 could be a novel independent prognostic factor of BCR after radical prostatectomy in the Chinese population. This functional polymorphism may also provide a basis for surveillance programs. Additional large-scale studies must be performed to validate the significance of this polymorphism in prostate cancer. PMID:27640814

  18. About the significance of biological factors affecting pregnancy a married couple

    Directory of Open Access Journals (Sweden)

    V. V. Yarman

    2013-01-01

    Full Text Available A brief review of the literature analysis of the importance and interdependence of the main factors influencing the occurrence of pregnancy as a pair in the treatment of infertility, such as the woman's age, ovarian reserve, the semen of a man and the duration of infertility. The review also presents the data concerning the practical implications of sexual constitution and sexual maturation in men and women in the pubertal period. When evaluating male fertility invariably significant factor in the prediction of pregnancy are, impregnating ability to ejaculate. Infertility is more common in males with delayed development in the pubertal period with a weak type of sexual constitution. Results of treatment of male infertility, the dynamics of the semen and pregnancy of the couple on the background of pathogenetic therapy correlates with the type of sexual constitution. Widely deployed discussion about the existence of the male equivalent of female menopause mainly concerns the problems of men's sexual health. Of exceptional importance for the prediction of treatment of infertility has ovarian reserve, which is closely associated with age women. Formation of menstrual function occurs in the pubertal period of sexual development, in this age of menarche is the leading symptom of type of sexual constitution of women that do not change throughout the life hereafter. The presence of menstrual function and even ovulation does not reflect the reproductive capacity of women. Insufficient information content of the chronological age of a woman, as an indicator of the reproductive capacity of ovarian dictates the necessity of the development of tests that determine individual biological age of a woman, perhaps connected with the type of sexual constitution. In this regard, the study of sexual constitution partners is of great scientific and practical interest.

  19. About the significance of biological factors affecting pregnancy a married couple

    Directory of Open Access Journals (Sweden)

    V. V. Yarman

    2014-11-01

    Full Text Available A brief review of the literature analysis of the importance and interdependence of the main factors influencing the occurrence of pregnancy as a pair in the treatment of infertility, such as the woman's age, ovarian reserve, the semen of a man and the duration of infertility. The review also presents the data concerning the practical implications of sexual constitution and sexual maturation in men and women in the pubertal period. When evaluating male fertility invariably significant factor in the prediction of pregnancy are, impregnating ability to ejaculate. Infertility is more common in males with delayed development in the pubertal period with a weak type of sexual constitution. Results of treatment of male infertility, the dynamics of the semen and pregnancy of the couple on the background of pathogenetic therapy correlates with the type of sexual constitution. Widely deployed discussion about the existence of the male equivalent of female menopause mainly concerns the problems of men's sexual health. Of exceptional importance for the prediction of treatment of infertility has ovarian reserve, which is closely associated with age women. Formation of menstrual function occurs in the pubertal period of sexual development, in this age of menarche is the leading symptom of type of sexual constitution of women that do not change throughout the life hereafter. The presence of menstrual function and even ovulation does not reflect the reproductive capacity of women. Insufficient information content of the chronological age of a woman, as an indicator of the reproductive capacity of ovarian dictates the necessity of the development of tests that determine individual biological age of a woman, perhaps connected with the type of sexual constitution. In this regard, the study of sexual constitution partners is of great scientific and practical interest.

  20. GDF11/BMP11 activates both smad1/5/8 and smad2/3 signals but shows no significant effect on proliferation and migration of human umbilical vein endothelial cells.

    Science.gov (United States)

    Zhang, Yong-Hui; Cheng, Feng; Du, Xue-Ting; Gao, Jin-Lai; Xiao, Xiao-Lin; Li, Na; Li, Shan-Liang; Dong, De Li

    2016-03-15

    GDF11/BMP11, a member of TGF-β superfamily, was reported to rejuvenate heart, skeletal muscle and blood vessel architecture in aged mice. However, the rejuvenative effects of GDF11 were questioned recently. Here, we investigated the effects of GDF11 on smad and non-smad signals in human umbilical vein endothelial cells (HUVECs) and the effects of GDF11 on proliferation and migration of HUVECs and primary rat aortic endothelial cells (RAECs). GDF11 factor purchased from two different companies (PeproTech and R&D Systems) was comparatively studied. Western blot was used to detect the protein expressions. The cell viability and migration were examined by using MTT and wound healing assays. Results showed that GDF11 activated both smad1/5/8 and smad2/3 signals in HUVECs. GDF11 increased protein expression of NADPH oxidase 4(NOX4) in HUVECs. GDF11 showed no significant effect on the protein level of p38, p-p38, ERK, p-ERK, Akt, p-Akt (Ser473) and p-Akt(Thr308), but increased the protein level of p-JNK and p-AMPK in HUVECs, and these increases were inhibited by antioxidant mitoTEMPO treatment. GDF11 slightly increased cell viability after short-term treatment and slightly decreased cell viability after long-term treatment. GDF11 showed no significant effect on cell proliferation and migration. These data indicated that the notion of GDF11 as a rejuvenation-related factor for endothelial cells needs to be cautious.

  1. Factors associated with an increased risk of vertebral fracture in monoclonal gammopathies of undetermined significance.

    Science.gov (United States)

    Piot, J M; Royer, M; Schmidt-Tanguy, A; Hoppé, E; Gardembas, M; Bourrée, T; Hunault, M; François, S; Boyer, F; Ifrah, N; Renier, G; Chevailler, A; Audran, M; Chappard, D; Libouban, H; Mabilleau, G; Legrand, E; Bouvard, B

    2015-08-28

    Monoclonal gammopathies of undetermined significance (MGUS) have been shown to be associated with an increased risk of fractures. This study describes prospectively the bone status of MGUS patients and determines the factors associated with vertebral fracture. We included prospectively 201 patients with MGUS, incidentally discovered, and with no known history of osteoporosis: mean age 66.6±12.5 years, 48.3% women, 51.7% immunoglobulin G (IgG), 33.3% IgM and 10.4% IgA. Light chain was kappa in 64.2% patients. All patients had spinal radiographs and bone mineral density measurement in addition to gammopathy assessment. At least one prevalent non-traumatic vertebral fracture was discovered in 18.4% patients and equally distributed between men and women. Fractured patients were older, had a lower bone density and had also more frequently a lambda light chain isotype. Compared with patients with κ light chain, the odds ratio of being fractured for patients with λ light chain was 4.32 (95% confidence interval 1.80-11.16; P=0.002). These results suggest a high prevalence of non-traumatic vertebral fractures in MGUS associated with lambda light chain isotype and not only explained by low bone density.

  2. Asymptomatic bacteriuria in women with autoimmune rheumatic disease: prevalence, risk factors, and clinical significance.

    Science.gov (United States)

    Georgiadou, Sarah P; Gamaletsou, Maria N; Mpanaka, Ioanna; Vlachou, Aggeliki; Goules, Andreas V; Ziogas, Dimitrios C; Syriou, Vassiliki; Tektonidou, Maria G; Kaltsas, Gregory; Manoussakis, Menelaos N; Sipsas, Nikolaos V

    2015-03-15

    Data regarding the prevalence and clinical significance of asymptomatic bacteriuria (AB) in women with autoimmune rheumatic disease (ARD) are scarce. In this prospective, case-control study, consecutive female outpatients with ARD were screened for AB. For each patient, demographics, type, duration, and treatment of underlying ARD, and risk factors for urinary tract infection (UTI), were recorded. Age-matched women with endocrine disease, without any autoimmune disease, not receiving immunosuppressive agents were used as controls. Subjects were followed up for 1 year for the development of symptomatic UTI. Two hundred sixty patients with ARD (mean age, 52.4 [standard deviation {SD}, 14.6] years) and 238 controls (mean age, 51.2 [SD, 16.5] years) were enrolled. The majority of patients with ARD (93.5%; 95% confidence interval [CI], 89.7%-95.9%) were receiving immunosuppressive agents. AB was detected in 24 patients with ARD (9.2%; 95% CI, 6.2%-13.4%) and in 22 controls (9.2%; 95% CI, 5.5%-12.9%) (P = 1.000). The most prevalent pathogen was Escherichia coli (16/24 [66%]). Independent predictors for AB among patients were diabetes (odds ratio [OR], 6.6; P = .008) and a longer ARD duration (>84 months; OR, 4.3; P = .018). During the 1-year follow-up, 9 patients with baseline AB remained persistently bacteriuric, whereas 11 were intermittently bacteriuric. Symptomatic UTI developed in 4 of 24 patients (16.7%; 95% CI, 6.1%-36.5%) with baseline AB vs 29 of 236 (12.3%; 95% CI, 8.6%-17.1%) without AB (P = .522). In our study, the prevalence of AB among women with ARD was not higher than that of controls, and AB was not associated with higher risk for symptomatic UTI. Risk factors for AB were longer duration of ARD and diabetes. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  3. Tumor necrosis factor-alpha allele 2 shows an association with insulin-dependent diabetes mellitus in Latvians.

    Science.gov (United States)

    Shtauvere-Brameus, A; Dabadghao, P; Rumba, I; Sanjeevi, C B

    2002-04-01

    Insulin-dependent diabetes mellitus (IDDM) is one of the most common chronic diseases. It is an autoimmune disease. Genes contributing the most for development of IDDM are located on chromosome 6p21.3 in the region called the major histocompatibility complex (MHC). HLA-DQ8/DR4 and DQ2/DR3 have shown positive association with IDDM, while DQ6 has negative association with IDDM in most Caucasian populations. The location of the tumor necrosis factor alpha (TNF-alpha) gene in the MHC suggests the role of TNF in the etiology of IDDM as an autoimmune disease. The TNF region contains several polymorphisms that are associated with different levels of TNF-alpha production and susceptibility to autoimmune and infectious diseases. Ninety-two Latvian IDDM patients corresponding to WHO diagnostic criteria and 107 unrelated age- and sex-matched healthy controls were analyzed for the frequency of TNF-alpha alleles to test the hypothesis that TNF-alpha is associated with IDDM. We found that TNF-alpha microsatellite allele 2 is associated with IDDM, 29/92 (32%), versus 14/107 (13%) in healthy controls. The test of the strongest association of the MICA A5 allele and TNF-alpha allele 2 with IDDM showed that both are independently associated with the disease.

  4. Plasma Levels of Macrophage Migration Inhibitory Factor and d-Dopachrome Tautomerase Show a Highly Specific Profile in Early Life

    Science.gov (United States)

    Roger, Thierry; Schlapbach, Luregn J.; Schneider, Anina; Weier, Manuela; Wellmann, Sven; Marquis, Patrick; Vermijlen, David; Sweep, Fred C. G. J.; Leng, Lin; Bucala, Richard; Calandra, Thierry; Giannoni, Eric

    2017-01-01

    Macrophage migration inhibitory factor (MIF) is a pleiotropic, constitutively expressed, pro-inflammatory cytokine and an important regulator of immune responses. d-dopachrome tautomerase (DDT), a newly described member of the MIF protein superfamily, shares sequence homology and biological activities with MIF. We recently reported that high expression levels of MIF sustain innate immune responses in newborns. Here, we elected to further characterize age-dependent MIF expression and to define whether DDT shares a similar expression profile with MIF. Therefore, we delineated the circulating concentrations of MIF and DDT throughout life using a large cohort of 307 subjects including fetuses, newborns, infants, children, and adults. Compared to levels measured in healthy adults (median: 5.7 ng/ml for MIF and 16.8 ng/ml for DDT), MIF and DDT plasma concentrations were higher in fetuses (median: 48.9 and 29.6 ng/ml), increased further at birth (median: 82.6 and 52.0 ng/ml), reached strikingly elevated levels on postnatal day 4 (median: 109.5 and 121.6 ng/ml), and decreased to adult levels during the first months of life. A strong correlation was observed between MIF and DDT concentrations in all age groups (R = 0.91, P < 0.0001). MIF and DDT levels correlated with concentrations of vascular endothelial growth factor, a protein upregulated under low oxygen tension and implicated in vascular and lung development (R = 0.70, P < 0.0001 for MIF and R = 0.65, P < 0.0001 for DDT). In very preterm infants, lower levels of MIF and DDT on postnatal day 6 were associated with an increased risk of developing bronchopulmonary dysplasia and late-onset neonatal sepsis. Thus, MIF and DDT plasma levels show a highly specific developmental profile in early life, supporting an important role for these cytokines during the neonatal period. PMID:28179905

  5. Corticortophin releasing factor 2 receptor agonist treatment significantly slows disease progression in mdx mice

    Directory of Open Access Journals (Sweden)

    Stevens Paula J

    2007-07-01

    Full Text Available Abstract Background Duchenne muscular dystrophy results from mutation of the dystrophin gene, causing skeletal and cardiac muscle loss of function. The mdx mouse model of Duchenne muscular dystrophy is widely utilized to evaluate the potential of therapeutic regimens to modulate the loss of skeletal muscle function associated with dystrophin mutation. Importantly, progressive loss of diaphragm function is the most consistent striated muscle effect observed in the mdx mouse model, which is the same as in patients suffering from Duchenne muscular dystrophy. Methods Using the mdx mouse model, we have evaluated the effect that corticotrophin releasing factor 2 receptor (CRF2R agonist treatment has on diaphragm function, morphology and gene expression. Results We have observed that treatment with the potent CRF2R-selective agonist PG-873637 prevents the progressive loss of diaphragm specific force observed during aging of mdx mice. In addition, the combination of PG-873637 with glucocorticoids not only prevents the loss of diaphragm specific force over time, but also results in recovery of specific force. Pathological analysis of CRF2R agonist-treated diaphragm muscle demonstrates that treatment reduces fibrosis, immune cell infiltration, and muscle architectural disruption. Gene expression analysis of CRF2R-treated diaphragm muscle showed multiple gene expression changes including globally decreased immune cell-related gene expression, decreased extracellular matrix gene expression, increased metabolism-related gene expression, and, surprisingly, modulation of circadian rhythm gene expression. Conclusion Together, these data demonstrate that CRF2R activation can prevent the progressive degeneration of diaphragm muscle associated with dystrophin gene mutation.

  6. Health-related quality of life of patients with rheumatoid arthritis. Which factors are of significance?

    DEFF Research Database (Denmark)

    Linde, L.; Sørensen, J.; Ostergaard, M.

    2008-01-01

    Rheumatoid arthritis (RA) is a chronic inflammatory disease causing joint pain, loss of function and decreased health-related quality of life (HRQoL). HRQoL in RA patients is associated with several risk factors; in this paper the evidence relating to the most important risk factors is reviewed....... Modern medical therapy has improved HRQoL in RA patients, while demographic factors (female sex and older age), low socioeconomic status (in terms of education and position in the work force) and the presence of comorbid conditions appear to be associated with poorer HRQoL Udgivelsesdato: 2008/3/3...

  7. Health-related quality of life of patients with rheumatoid arthritis. Which factors are of significance?

    DEFF Research Database (Denmark)

    Linde, L.; Sørensen, J.; Ostergaard, M.;

    2008-01-01

    . Modern medical therapy has improved HRQoL in RA patients, while demographic factors (female sex and older age), low socioeconomic status (in terms of education and position in the work force) and the presence of comorbid conditions appear to be associated with poorer HRQoL Udgivelsesdato: 2008/3/3......Rheumatoid arthritis (RA) is a chronic inflammatory disease causing joint pain, loss of function and decreased health-related quality of life (HRQoL). HRQoL in RA patients is associated with several risk factors; in this paper the evidence relating to the most important risk factors is reviewed...

  8. Structure shows that a glycosaminoglycan and protein recognition site in factor H is perturbed by age-related macular degeneration-linked single nucleotide polymorphism.

    Science.gov (United States)

    Herbert, Andrew P; Deakin, Jon A; Schmidt, Christoph Q; Blaum, Bärbel S; Egan, Claire; Ferreira, Viviana P; Pangburn, Michael K; Lyon, Malcolm; Uhrín, Dusan; Barlow, Paul N

    2007-06-29

    A common single nucleotide polymorphism in the factor H gene predisposes to age-related macular degeneration. Factor H blocks the alternative pathway of complement on self-surfaces bearing specific polyanions, including the glycosaminoglycan chains of proteoglycans. Factor H also binds C-reactive protein, potentially contributing to noninflammatory apoptotic processes. The at risk sequence contains His (rather than Tyr) at position 402 (384 in the mature protein), in the seventh of the 20 complement control protein (CCP) modules (CCP7) of factor H. We expressed both His(402) and Tyr(402) variants of CCP7, CCP7,8, and CCP6-8. We determined structures of His(402) and Tyr(402) CCP7 and showed them to be nearly identical. The side chains of His/Tyr(402) have similar, solvent-exposed orientations far from interfaces with CCP6 and -8. Tyr(402) CCP7 bound significantly more tightly than His(402) CCP7 to a heparin affinity column as well as to defined-length sulfated heparin oligosaccharides employed in gel mobility shift assays. This observation is consistent with the position of the 402 side chain on the edge of one of two glycosaminoglycan-binding surface patches on CCP7 that we inferred on the basis of chemical shift perturbation studies with a sulfated heparin tetrasaccharide. According to surface plasmon resonance measurements, Tyr(402) CCP6-8 binds significantly more tightly than His(402) CCP6-8 to immobilized C-reactive protein. The data support a causal link between H402Y and age-related macular degeneration in which variation at position 402 modulates the response of factor H to age-related changes in the glycosaminoglycan composition and apoptotic activity of the macula.

  9. Identifying significant genetic regulatory networks in the prostate cancer from microarray data based on transcription factor analysis and conditional independency

    Directory of Open Access Journals (Sweden)

    Yeh Cheng-Yu

    2009-12-01

    Full Text Available Abstract Background Prostate cancer is a world wide leading cancer and it is characterized by its aggressive metastasis. According to the clinical heterogeneity, prostate cancer displays different stages and grades related to the aggressive metastasis disease. Although numerous studies used microarray analysis and traditional clustering method to identify the individual genes during the disease processes, the important gene regulations remain unclear. We present a computational method for inferring genetic regulatory networks from micorarray data automatically with transcription factor analysis and conditional independence testing to explore the potential significant gene regulatory networks that are correlated with cancer, tumor grade and stage in the prostate cancer. Results To deal with missing values in microarray data, we used a K-nearest-neighbors (KNN algorithm to determine the precise expression values. We applied web services technology to wrap the bioinformatics toolkits and databases to automatically extract the promoter regions of DNA sequences and predicted the transcription factors that regulate the gene expressions. We adopt the microarray datasets consists of 62 primary tumors, 41 normal prostate tissues from Stanford Microarray Database (SMD as a target dataset to evaluate our method. The predicted results showed that the possible biomarker genes related to cancer and denoted the androgen functions and processes may be in the development of the prostate cancer and promote the cell death in cell cycle. Our predicted results showed that sub-networks of genes SREBF1, STAT6 and PBX1 are strongly related to a high extent while ETS transcription factors ELK1, JUN and EGR2 are related to a low extent. Gene SLC22A3 may explain clinically the differentiation associated with the high grade cancer compared with low grade cancer. Enhancer of Zeste Homolg 2 (EZH2 regulated by RUNX1 and STAT3 is correlated to the pathological stage

  10. The genome-wide binding profile of the Sulfolobus solfataricus transcription factor Ss-LrpB shows binding events beyond direct transcription regulation.

    Science.gov (United States)

    Nguyen-Duc, Trong; van Oeffelen, Liesbeth; Song, Ningning; Hassanzadeh-Ghassabeh, Gholamreza; Muyldermans, Serge; Charlier, Daniel; Peeters, Eveline

    2013-11-25

    Gene regulatory processes are largely resulting from binding of transcription factors to specific genomic targets. Leucine-responsive Regulatory Protein (Lrp) is a prevalent transcription factor family in prokaryotes, however, little information is available on biological functions of these proteins in archaea. Here, we study genome-wide binding of the Lrp-like transcription factor Ss-LrpB from Sulfolobus solfataricus. Chromatin immunoprecipitation in combination with DNA microarray analysis (ChIP-chip) has revealed that Ss-LrpB interacts with 36 additional loci besides the four previously identified local targets. Only a subset of the newly identified binding targets, concentrated in a highly variable IS-dense genomic region, is also bound in vitro by pure Ss-LrpB. There is no clear relationship between the in vitro measured DNA-binding specificity of Ss-LrpB and the in vivo association suggesting a limited permissivity of the crenarchaeal chromatin for transcription factor binding. Of 37 identified binding regions, 29 are co-bound by LysM, another Lrp-like transcription factor in S. solfataricus. Comparative gene expression analysis in an Ss-lrpB mutant strain shows no significant Ss-LrpB-mediated regulation for most targeted genes, with exception of the CRISPR B cluster, which is activated by Ss-LrpB through binding to a specific motif in the leader region. The genome-wide binding profile presented here implies that Ss-LrpB is associated at additional genomic binding sites besides the local gene targets, but acts as a specific transcription regulator in the tested growth conditions. Moreover, we have provided evidence that two Lrp-like transcription factors in S. solfataricus, Ss-LrpB and LysM, interact in vivo.

  11. THE EXPRESSIONS AND SIGNIFICANCES OF p15,p16 AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) IN GASTRIC CARCINOMA

    Institute of Scientific and Technical Information of China (English)

    王天宝; 高鹏; 曲延刚; 陈咸增; 李兆亭

    2000-01-01

    Objective:To investigate the relationships between the expressions of p15,p16 and vascular endothelial growth factor (VEGF) and gastric carcinoma(GC).Methods: Using immunohistochemical staining to examine the expressions of p15, p16and VEGF in archival wax-embedded specimens of 80 GC and 20 gastric benign disease(GBD). Results: The positive expression rate (PER) of p15 was significantly lower in GC than in GBD (43.75% VS. 69.23%, P<0.05). No relationship was found between PER of p15 and clinicopathologic factors. PER of p16 was 20% in GC, 55% in GBD (P<0.01).PER of p16 wasn't significantly different in gross types, histological types, with or without distant metastasis and pTNM stages. PER of p16 was 71.43% in invasive mucosa or suomucosa group, 17. 24% in invasive muscle group and 13. 64% in invoive serosa group (P<0.01); 12.96% in GC with lymph nodes metastasis, 34.62% in GC without lymph node metastasis (P<0.05). PER of VEGF in GC was 75. 00%, in GBD 7.69% (P<0. 001), in ulcerative type of GC and infiltrating type of GC were 81.97% and 40. 00%, respectively (P <0.05), in GC of invasive serosa was 95.45%, in GC of invasive muscle 51.72%(P<0.001), in GC of invasive mucosa or sulomucosa 42.86% (P<0.001). PER of VEGF in GC with lymph node metastasis was 82. 8%, without lymph node metastasis 54. 6%(P<0.05), in GC accompanied with distant metastasis was 100%, in GC without distant metastasis71.1% (P<0.05). PER of VEGF in pTNM Ⅰ and Ⅱ was 53.13%, in Ⅲ and IV 89.56% (P<0. 001). The expression of p15 correlated significantly With that of VEGF (P<0.001) and with that of p16 (P<0.01) in GC. Conclusion: p15 expression down-regulation has relationship with GC, but on relationship with the progress. p16 expression downregulation and VEGF expression up-regulation show significant relationships with clinicopathologic factors. There are significant relations between p15 and p16 negative expressionsand between p15 expression down-regulation and VEGF expression up-regulation.

  12. [Triglycerides--a long known risk factor for cardiovascular disease. Subgroup analysis shows the importance after acute coronary syndrome].

    Science.gov (United States)

    Olsson, Anders

    2015-09-09

    An increased blood concentration of triglycerides (TG) has long been recognized as an important risk factor for cardiovascular disease. Through competition from HDL cholesterol and the arrival of statin treatment for high LDL cholesterol the importance of TG as risk factor was largely forgotten. A high concentration of TG indicates high blood levels of TG-rich lipoproteins including cholesterol rich remnant particles. Studies using Mendelian randomizations have demonstrated that a low HDL cholesterol does not carry a direct atherogenic function and that remnant particles do so. New efforts should be exercised in order to diminish residual cardiovascular risk during statin treatment through decreasing TG rich lipoproteins.

  13. A Cyclodissipativity Condition for Power Factor Improvement under Nonsinusoidal Source with Significant Impedance

    NARCIS (Netherlands)

    Puerto-Flores, Dunstano del; Ortega, Romeo; Scherpen, Jacquelien M.A.

    2010-01-01

    The main contribution of this paper is an extension of a recent result that reformulates and solves the power factor compensation for nonlinear loads under nonsinusoidal regime in terms of cyclodissipativity. In the aforementioned result the generator was assumed to be ideal, that is, with negligibl

  14. Risk Factors for Persistent Fatigue With Significant School Absence in Children and Adolescents

    NARCIS (Netherlands)

    Bakker, Robert J.; van de Putte, Elise M.; Kuis, Wietse; Sinnema, Gerben

    2009-01-01

    OBJECTIVE: To assess children and adolescents with severe fatigue who are referred to pediatricians and to examine whether factors can be identified at their first visit that predict worse outcomes at 1 year. METHODS: Ninety-one patients, aged 8 to 18 years completed questionnaires about sleep, soma

  15. Related factors and prognostic significance of intradialytic blood pressure variability in patients on maintenance hemodialysis

    Institute of Scientific and Technical Information of China (English)

    谷立杰

    2013-01-01

    Objective To evaluate intradialytic blood pressure variability (BPV) in patients on maintenance hemodialysis (MHD) ,and to investigate the correlated factors of BPV in MHD process and its correlation with prognosis.Methods Patients with end stage renal disease on MHD before January 1,2009 were enrolled and analyzed retro-

  16. Exploring matrix factorization techniques for significant genes identification of Alzheimer’s disease microarray gene expression data

    Directory of Open Access Journals (Sweden)

    Hu Xiaohua

    2011-07-01

    Full Text Available Abstract Background The wide use of high-throughput DNA microarray technology provide an increasingly detailed view of human transcriptome from hundreds to thousands of genes. Although biomedical researchers typically design microarray experiments to explore specific biological contexts, the relationships between genes are hard to identified because they are complex and noisy high-dimensional data and are often hindered by low statistical power. The main challenge now is to extract valuable biological information from the colossal amount of data to gain insight into biological processes and the mechanisms of human disease. To overcome the challenge requires mathematical and computational methods that are versatile enough to capture the underlying biological features and simple enough to be applied efficiently to large datasets. Methods Unsupervised machine learning approaches provide new and efficient analysis of gene expression profiles. In our study, two unsupervised knowledge-based matrix factorization methods, independent component analysis (ICA and nonnegative matrix factorization (NMF are integrated to identify significant genes and related pathways in microarray gene expression dataset of Alzheimer’s disease. The advantage of these two approaches is they can be performed as a biclustering method by which genes and conditions can be clustered simultaneously. Furthermore, they can group genes into different categories for identifying related diagnostic pathways and regulatory networks. The difference between these two method lies in ICA assume statistical independence of the expression modes, while NMF need positivity constrains to generate localized gene expression profiles. Results In our work, we performed FastICA and non-smooth NMF methods on DNA microarray gene expression data of Alzheimer’s disease respectively. The simulation results shows that both of the methods can clearly classify severe AD samples from control samples, and

  17. [Chlamydia trachomatis proteasome protein as one of the significant pathogenicity factors of exciter].

    Science.gov (United States)

    Davydov, D Iu; Zigangirova, N A

    2014-01-01

    Sex-related infections are a global problem. Such infections may lead to acute or chronic diseases. Chlamydia trachomatis is a dangerous and widespread pathogenicity factor that is not sensitive to conventional drugs and has no obvious symptoms. Protein CPAF is leading factor of pathogenesis. This protein inhibits the signaling pathways of host cell and supports long survival of the pathogen in the host cell. The goal of this work was to review general properties of the proteasome Chlamydia protein CPAF, its functions, and role in pathology. The role of protein CPAF in the anti-chlamydia immune reaction is discussed. The prospects of the development of promising anti-chlamydia vaccine, as well as new effective anti-chlamydia drugs are also discussed.

  18. ORGANIZATIONAL CULTURE AS SIGNIFICANT FACTOR IN IMPLEMENTATION OF TQM - EXPERIENCE IN SERBIAN ECONOMY

    Directory of Open Access Journals (Sweden)

    Vladimir Nikolić

    2010-03-01

    Full Text Available The theory has confirmed the view that the appropriate organizational culture enables success in implementation of Total Quality Management (TQM. Conducted research points on characteristics of organizational culture in the relevant sample of enterprises in Serbia. The factors of the organization that affect on shaping the organizational culture of the business system were separated by using analytical approach. This creates the basis for the designing model of necessary changes in the organizational culture that ensures successful implementation of TQM.

  19. [Early risk factors of eating disorders--do events of prenatal and perinatal periods bear significance?].

    Science.gov (United States)

    Raevuori, Anu; Niemelä, Solja; Keski-Rahkonen, Anna; Sourander, Andre

    2009-01-01

    Evidence of the relation of complications occurring in the pregnancy, delivery and neonatal periods to the risk of contracting a subsequent eating disorder has been obtained during recent years. Factors associated with parturition and neonatal period seem to predict both anorexia and bulimia, whereas disorders during pregnancy are more clearly associated with the descendant's anorexia. This difference may play an important role in the pathogenesis of the disorders.

  20. Which factors are significant for obtaining business intelligence success in the public sector?

    DEFF Research Database (Denmark)

    Gaardboe, Rikke

    2017-01-01

    The objective of this paper is to present a brief introduction to the doctoral thesis of the author. The content of the thesis identifies the critical success factors for obtaining business intelligence success, measured as use, user satisfaction, net benefits and individual impact from an end user...... and the perceived individual impact of using business intelligence in the public sector. This PhD bridges a gap in obtaining an understanding of what tasks and quality fit the use of business intelligence....

  1. Suicide Risk Assessments: Which Suicide Risk Factors Psychiatric Residents Consider Significant?

    OpenAIRE

    Wang, Sheng-Min; Hwang, Sunyoung; Yeon, Bora; Choi, Kyoung Ho; Oh, Youngmin; Lee, Hae-Kook; Kweon, Yong-Sil; Lee, Chung Tai; Lee, Kyoung-Uk

    2015-01-01

    Objective Patients visiting the emergency department (ED) after a suicide attempt are generally assessed for suicide risk by psychiatric residents. Psychiatric residents' competence in evaluating the risk posed by the patients who attempted suicide is critical to preventing suicide. Methods We investigated factors considered important by psychiatric residents when evaluating suicide risk. This study included 140 patients admitted to the ED after attempting suicide. Psychiatric residents rated...

  2. THE IMAGE OF SIGNIFICANT OTHER AS A FACTOR OF ELDERLY PEOPLE’S LIFE SATISFACTION

    OpenAIRE

    Natalya Vladimirovna Khalina

    2013-01-01

    Purpose. The article looks at the phenomenon of significant Other as an element of self-consciousness and its correlation with life satisfaction of elderly people. The author of the article draws special attention to the interrelation of life satisfaction and the Image of significant Other for elderly people which is of current interest in connection with the importance of developing co-existence relationships with the Others.Research Methods: theoretical study method; techniques of data proc...

  3. Identifying principal risk factors for the initiation of adolescent smoking behaviors: the significance of psychological reactance.

    Science.gov (United States)

    Miller, Claude H; Burgoon, Michael; Grandpre, Joseph R; Alvaro, Eusebio M

    2006-01-01

    An in-school youth survey for a major state anti-tobacco media campaign was conducted with 1,831 students (Grades 6-12) from 70 randomly selected classrooms throughout the state. Tobacco users accounted for nearly 25% of the sample. Pretest questionnaires assessed demographic variables, tobacco use, and various other risk factors. Several predictors of adolescents' susceptibility to tobacco use, including prior experimentation with tobacco, school performance, parental smoking status, parents' level of education, parental communication, parental relationship satisfaction, best friend's smoking status, prevalence of smokers in social environment, self-perceived potential to smoke related to peer pressure, and psychological reactance, were examined using discriminant analysis and logistic regression to identify the factors most useful in classifying adolescents as either high-risk or low-risk for smoking uptake. Results corroborate findings in the prevention literature indicating that age, prior experimentation, and having friends who smoke are among the principal predictors of smoking risk. New evidence is presented indicating that psychological reactance also should be considered as an important predictor of adolescent smoking initiation. The utility of producing antismoking messages informed by an awareness of the key risk factors-particularly psychological reactance-is discussed both in terms of the targeting and design of anti-tobacco campaigns.

  4. Obesity is a significant risk factor for breast cancer in Arab women.

    Science.gov (United States)

    Elkum, Naser; Al-Tweigeri, Taher; Ajarim, Dahish; Al-Zahrani, Ali; Amer, Suad M Bin; Aboussekhra, Abdelilah

    2014-10-29

    Breast cancer (BC) is the most common malignancy and the leading cause of cancer-related death amongst women worldwide. The risk factors of this disease are numerous, and their prevalence varies between racial and ethnic groups as well as geographical regions. Therefore, we sought to delineate the association of socio-demographic, reproductive and life-style related risk factors with breast cancer in the Arab population. Unmatched case-control study was conducted in the kingdom of Saudi Arabia using 534 cases of histologically confirmed breast cancer and 638 controls. Controls were randomly selected from primary health care visits and were free of breast cancer. Unconditional logistic regression analysis was performed to estimate odds ratios (ORs) and to examine the predictive effect of each factor on risk for BC. All study participants were interviewed by trained interviewers at hospital (cases) or at primary health care centers (controls). A total of 1172 women were eligible for this study, of which 281 (24.0%) were aged ≤35 years, 22.9% illiterate, 43.6% employed, 89.5% married, and 38.1% were obese. Grade III tumors constituted 38.4% of cases. Tumor stage I was 7.5%; II, 50.7%; II, 30.9%; IV, 11.1%. We have shown strong association between breast cancer among Arab females and obesity (OR =2.29, 95% CI 1.68-3.13), positive family history of breast cancer (OR =2.31, 95% CI 1.60 - 3.32), the use of hormonal replacement therapy (OR =2.25, 95% CI 1.65 - 3.08), post-menopause (OR =1.72, 95% CI 1.25 - 2.38), lack of education (OR =9.09, 95% CI 5.88 - 14.29), and never breastfeed (OR =1.89, 95% CI 1.19 - 2.94). These results indicate the presence of classical risk factors established in the western countries, and also some specific ones, which may result from genetic and/or environmental factors. Thereby, these findings will be of great value to establish adequate evidence-based awareness and preventative measures in the Arab world.

  5. EARLY ALLOGRAFT DYSFUNCTION AND ACUTE KIDNEY INJURY AFTER LIVER TRANSPLANTATION: DEFINITIONS, RISK FACTORS AND CLINICAL SIGNIFICANCE

    Directory of Open Access Journals (Sweden)

    L. Y. Moysyuk

    2012-01-01

    Full Text Available This review discusses issues related to intensive care in recipients of transplanted liver in the early postoperative period, with an emphasis on contemporary conditions and attitudes that are specific for this group of patients. Early allograft dysfunction (EAD requires immediate diagnosis and appropriate treatment in case. The causes of the EAD and therapeutic tactics are discussed. Acute kidney injury (AKI and renal failure are common in patients after transplantation. We consider etiology, risk factors, diagnosis and treatment guidelines for AKI. The negative impact of EAD and AKI on the grafts survival and recipients is demonstrated. 

  6. [Factors significant for cerebral circulacion in patients with supratentorial brain tumors].

    Science.gov (United States)

    Sboev, A Yu; Dolgih, V T; Larkin, V I

    2013-01-01

    Using the Doppler ultrasonography method the condition of brain blood circulation of 90 patients with supratentorial brain tumors (gliomas--43, meningiomas--34, metastasis--9) during pre-surgical period was studied. The factors changing brain blood circulation at patients with with supratentorial brain tumors were brain displacement, increase of intracranial pressure, histologic structure and the first symptoms duration of illness. Localization (for an exception of an occipital lobe) and the size of a tumor directly didn't render influence on blood circulation parameters.

  7. Advancing the science of nursing education: rethinking the meaning and significance of impact factors.

    Science.gov (United States)

    Ironside, Pamela M

    2007-01-01

    It is not the case that impact factors are unhelpful and that their use should be abolished. Indeed, ISI is continuing to refine existing and develop new databases to provide important information to researchers, administrators, librarians, and editors. Impact factor data do provide useful information for the review process if used judiciously and with an awareness of what these data do and do not indicate. Perhaps it is timely for members of the nursing discipline to think more broadly about the nature of impact and to talk more about the ways in which review processes can account for the many ways the impact of research can be demonstrated. For example, as part of the review process, reviewers might ask each researcher to provide exemplars from teaching or clinical practice settings in which their research is actually being used. Researchers might also be asked to describe the impact that has occurred in ways other than through publication (e.g., presentations, consulting) and how this impact was determined. Creating review processes that allow researchers to describe their decision making related to disseminating their research and how this reflects their ability to influence the discipline may provide a more realistic picture of impact than calculated figures alone.

  8. The significance of oral and systemic factors in Australian and Croatian patients with oral lichen planus.

    Science.gov (United States)

    Vučićević Boras, Vanja; Savage, Neil William; Brailo, Vlaho; Škrinjar, Ivana; Valter, Kruna; Alajbeg, Iva; Dulčić, Nikša; Vidović Juras, Danica

    2014-01-01

    Oral lichen planus (OLP) is an immunologically T cell-mediated disease caused by an unknown stimulus. Despite intensive investigation its pathogenesis still remains unknown. A few possible associations between OLP and certain diseases such as thyroid and malignant diseases as well as specific medication intake have been proposed in the literature with inconsistent findings. We aimed to investigate the profile of 163 Australian and 163 Croatian OLP patients with special regard to their systemic diseases, medication intake (with special regard to the drugs that metabolize through Cytochrome P450), OLP type and localization, as well as involvement of other body surfaces with lichen. We did not find any statistical significance with regard to the OLP presence and thyroid and malignant diseases. As expected, the reticular type of OLP was most prevalent, as well as involvement of the both buccal mucosas. There was no significant association with other oral diseases such as labial herpes. Simultaneous involvement of other body surfaces in patients with OLP does not seem to be prevalent. None of the medical conditions which were investigated had significant correlation with OLP neither in Australian nor in Croatian patients with OLP. Furthermore, the use of drugs which metabolize through Cytochrome P450 (CYP450) was not significantly correlated with OLP in either studied population. Therefore, we conclude that patients with OLP are not to be routinely screened for any systemic conditions.

  9. THE IMAGE OF SIGNIFICANT OTHER AS A FACTOR OF ELDERLY PEOPLE’S LIFE SATISFACTION

    Directory of Open Access Journals (Sweden)

    Natalya Vladimirovna Khalina

    2013-10-01

    Full Text Available Purpose. The article looks at the phenomenon of significant Other as an element of self-consciousness and its correlation with life satisfaction of elderly people. The author of the article draws special attention to the interrelation of life satisfaction and the Image of significant Other for elderly people which is of current interest in connection with the importance of developing co-existence relationships with the Others.Research Methods: theoretical study method; techniques of data processing, Fisher’s ratio test, Spearman rank correlation analysis.Results. Elderly people’s life satisfaction is connected with the Image of significant Other: for satisfied with their life elderly people – with self-respect, for dissatisfied – with self-confidence.  Self-respect and self-confidence of the significant Other increases elderly people’s life satisfaction. Moreover, the vision of values of the Other (communication values, self-actualization, business is a crucial parameter linked to elderly people’s life satisfaction.Results Application. The study findings can be used to develop courses on psychology of personality, developmental psychology, gerontopsychology, counseling and psychocorrection, the findings can be also useful for social service centers providing aid for the elderly.DOI: http://dx.doi.org/10.12731/2218-7405-2013-7-33

  10. Obesity is a significant risk factor for prostate cancer at the time of biopsy.

    Science.gov (United States)

    Freedland, Stephen J; Wen, Joanne; Wuerstle, Melanie; Shah, Amy; Lai, Dominic; Moalej, Bita; Atala, Christina; Aronson, William J

    2008-11-01

    Studies suggest obesity is associated with decreased prostate cancer risk. We hypothesized obesity is biologically associated with increased risk, although this is obscured owing to hemodilution of prostate-specific antigen (PSA) and larger prostate size. We retrospectively studied 441 consecutive men undergoing prostate biopsy between 1999 and 2003 at two equal access centers within the Veterans Affairs Greater Los Angeles Healthcare System. We estimated the association between obesity (body mass index >or= 30 kg/m(2)) and positive biopsy and Gleason >or=4+3 using logistic regression analysis adjusting for multiple clinical characteristics. A total of 123 men (28%) were obese and 149 men (34%) had cancer. Median PSA and age were 5.7 ng/mL and 63.9 years, respectively. Obese men had significantly lower PSA concentrations (P = .02) and larger prostate volumes (P = .04). Obesity was not significantly related to age (P = .19) or race (P = .37). On univariate analysis, obesity was not associated with prostate cancer risk (odds ratio [OR] 1.13, 95% confidence interval [CI] 0.73-1.75, P = .58). However, after adjusting for multiple clinical characteristics, obesity was associated with significantly increased prostate cancer risk (OR 1.98, 95% CI 1.17-3.32, P = .01). After multivariable adjustment, there was no significant association between obesity and high-grade disease (P = .18). Without adjustment for clinical characteristics, obesity was not significantly associated with prostate cancer risk in this equal-access, clinic-based population. However, after adjusting for the lower PSA levels and the larger prostate size, obesity was associated with a 98% increased prostate cancer risk. These findings support the fact that current prostate cancer screening practices may be biased against obese men.

  11. Clinical Significance of Tissue Factor-Exposing Microparticles in Arterial and Venous Thrombosis.

    Science.gov (United States)

    van Es, Nick; Bleker, Suzanne; Sturk, Auguste; Nieuwland, Rienk

    2015-10-01

    Microparticles (MP) are small extracellular vesicles (30-1,000 nm) that are released from activated cells or platelets. Exposure of negatively charged phospholipids and tissue factor (TF) renders MP procoagulant. Normal plasma levels of intravascular TF-exposing MP (TFMP) are low, but their number may rise in pathological conditions, including cancer and infectious disease. Emerging evidence indicates an important role for these circulating TFMP in the pathogenesis of thrombotic complications such as venous thromboembolism and disseminated intravascular coagulation, whereas their contribution to arterial thrombosis is less studied. Despite serious limitations of the currently available assays for measuring TFMP levels or the procoagulant activity associated with TFMP with respect to sensitivity and specificity, the scientific interest in TFMP is rapidly growing because their application as prognostic biomarkers for thrombotic complications is promising. Future advances in detection methods will likely provide more insight into TFMP and eventually improve their clinical utility.

  12. Clinical significance of vascular endothelial growth factor expression in patients with carcinoma of the mouth floor and tongue

    Directory of Open Access Journals (Sweden)

    Broćić Miroslav

    2009-01-01

    Full Text Available Background/Aim. Although there are several types of malignant oral cancers, more than 90% of all diagnosed oral cancers are squamous cell carcinoma (OSCC. Angiogenesis is a cascade-like mechanism which is essential for tumor growth and metastasis. Therefore, vascular endothelial growth factor (VEGF expression in OSCC and its effect on clinicopathological characteristics and prognosis is of major interest. So far researches have shown that increased expression of this gene, in other words enhanced synthesis of this protein (VEGF, independently on other factors, increases a chance for local relapse, and distant metastasis. Consequently, patients with OSCC have poor disease-free survival, as well as poor overall survival. The aim of the study was to determine clinical significance of VEGF expression in patients with stage II and III OSCC. Methods. This retrospective study analyzed 40 patients who had been operated for OSCC of their tongue and the mouth floor. Of these patients, some had stage II and III OSCC with histological grade, G1-G3 and nuclear grade Ng1-Ng3. Two high quality tissue samples were obtained and immunohistochemical expression of VEGF was quantitatively determined by using high microscope amplification. The value of VEGF expression of 20% was rated as significant expression, whereas tumor cells reactivation less than 20% was considered very low or no expression at all. The patients were followed up for a 3-year period. Results. The obtained results showed that 11 (17.5% patients had VEGF expression less than 20% and 29 (82.5% above 20%. A statistical significance was immanent with positive nodal status (p < 0.05 and disease stage (p < 0.05. No statistical correlation was found between the level of VEGF expression and histological and nuclear grade, tumor size, disease relapse or patients overall survival. Conclusion. In spite the controversy about the prognostic relevance of VEGF our results as well as the results of previous

  13. Expression of early growth response factor-1 in rats with cerulein-induced acute pancreatitis and its significance

    Institute of Scientific and Technical Information of China (English)

    Lan-Bo Gong; Li He; Yang Liu; Xue-Qing Chen; Bo Jiang

    2005-01-01

    AIM: To observe the expressions of early growth response factor-1 (Egr-L) and tissue factor (TF) in rats with cerulein-induced acute pancreatitis and to explore its significance.METHODS: A large dose of cerulein was used to create the experimental acute pancreatitis model in rats. The changes of Egr-1 mRNA and protein in rats were observed during 30 min to 4 h after the treatment and immunohistochemical method was used to observe the localized expression of Egr-1 in tissues. In addition to the mRNA expression of Egr-1 target gene, TF was also observed. A blank control group, and a bombesinadministered group were used for comparison.RESULTS: After the stimulation of a large dose of cerulein,the rats showed typical inflammatory changes of acute pancreatitis. Thirty minutes after the stimulation, the mRNA expression of Egr-1 in the pancreatic tissue reached its peak and then declined, while the expression of Egr-1protein reached its peak 2 h after the stimulation.Histologically, 2 h after the stimulation, almost all pancreatic acinar cells had the expression of Egr-1 protein,which was focused in the nuclei. The mRNA expression of TF occurred 1 h after the stimulation and gradually increased within 4 h. However, a large dose of bombesin only stimulated the pancreatic tissue to produce a little mRNA expression of Egr-1 and no mRNA expression of Egr-1 protein and TF.CONCLUSION: Egr-1 as a pro-inflammatory transcription factor may play an important role in the pathogenesis of acute pancreatitis by modulating the expression of TF.

  14. Parameters Obtained by Hepatobiliary Scintigraphy Have Significant Correlation with Biochemical Factors Early After Liver Transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Kansoul, H.A.; Axelsson, R.; Yamamoto, S.; Savicheva, I.; Aspelin, P.; Ericzon, B.G.; Gjertsen, H. [Div. of Transplantation Surgery and Div. of Radiology, Dept. of Clinical Science, Intervention, and Technology, Karolinska Inst., Karolinska Univ. Hospital, Stockholm (Sweden)

    2007-08-15

    Background: Early postoperative hepatobiliary scintigraphy after liver transplantation is performed worldwide, but data on its significance for graft function are currently limited. Purpose: To examine the correlation between the result of early postoperative hepatobiliary scintigraphy and pre- and postoperative biochemical parameters in liver transplantation (LTx) patients. Material and Methods: Six parameters of hepatobiliary scintigraphy using {sup 99m}Tc mebrofenin were statistically analyzed in 108 LTx patients: 1) half-life of the activity of elimination of mebrofenin from the blood; 2) total clearance of mebrofenin from the blood due to all possible routes; 3) half-life of the activity due to liver uptake; 4) clearance of mebrofenin from the blood due to liver uptake; 5) time to maximal uptake in the liver; and 6) the hepatic extraction fraction (HEF) and biochemical data. Analysis between patients with preoperative normal liver function, familial amyloid polyneuropathy (FAP), and end-stage liver disease (non-FAP) was also performed. Results: Univariate and multivariate analysis revealed that total bilirubin postoperative day 3 correlated with all three scintigraphic parameters, and peak aspartate aminotransferase and alanine aminotransferase correlated with HEF. The analysis between patients with FAP and non-FAP revealed no significant difference of scintigraphic data between the two groups. Conclusion: A significant correlation between early postoperative scintigraphic results and biochemical parameters was demonstrated.

  15. Factors of Significant Impact on Proficiency Levels of Adult ESL Learners within Post-Secondary Education in Puerto Rico

    Science.gov (United States)

    Perez, Ramon

    2011-01-01

    This qualitative case study seeks to identify factors that have a significant impact on the second language proficiency levels of adult English as a Second Language (ESL) learners at a four-year university in Puerto Rico. Current data indicate that a significant percentage of adult ESL learners encounter major difficulties within the process of…

  16. The Impact of Stress on Tumor Growth; the Significance of Peripheral Corticotropin Releasing Factor

    Science.gov (United States)

    2009-05-01

    visualize the architecture of actin in the cell. Cells treated with CRF showed more intense staining compared to the untreated controls, most extensively...time points. Tumor growth was monitored every 4 days using a Fluorescent Molecular Tomography approach ( FMT ) (13) as shown in Figure 12. During the... FMT analysis of the tumors revealed that in mice not exposed to stress, administration of antalarmin resulted in reduced tumor burden. Upon stress

  17. Factors regulating the Great Calcite Belt in the Southern Ocean and its biogeochemical significance

    Science.gov (United States)

    Balch, William M.; Bates, Nicholas R.; Lam, Phoebe J.; Twining, Benjamin S.; Rosengard, Sarah Z.; Bowler, Bruce C.; Drapeau, Dave T.; Garley, Rebecca; Lubelczyk, Laura C.; Mitchell, Catherine; Rauschenberg, Sara

    2016-08-01

    The Great Calcite Belt (GCB) is a region of elevated surface reflectance in the Southern Ocean (SO) covering 16% of the global ocean and is thought to result from elevated, seasonal concentrations of coccolithophores. Here we describe field observations and experiments from two cruises that crossed the GCB in the Atlantic and Indian sectors of the SO. We confirm the presence of coccolithophores, their coccoliths, and associated optical scattering, located primarily in the region of the subtropical, Agulhas, and Subantarctic frontal regions. Coccolithophore-rich regions were typically associated with high-velocity frontal regions with higher seawater partial pressures of CO2 (pCO2) than the atmosphere, sufficient to reverse the direction of gas exchange to a CO2 source. There was no calcium carbonate (CaCO3) enhancement of particulate organic carbon (POC) export, but there were increased POC transfer efficiencies in high-flux particulate inorganic carbon regions. Contemporaneous observations are synthesized with results of trace-metal incubation experiments, 234Th-based flux estimates, and remotely sensed observations to generate a mandala that summarizes our understanding about the factors that regulate the location of the GCB.

  18. Peat decomposition - shaping factors, significance in environmental studies and methods of determination; a literature review

    Science.gov (United States)

    Drzymulska, Danuta

    2016-03-01

    A review of literature data on the degree of peat decomposition - an important parameter that yields data on environmental conditions during the peat-forming process, i.e., humidity of the mire surface, is presented. A decrease in the rate of peat decomposition indicates a rise of the ground water table. In the case of bogs, which receive exclusively atmospheric (meteoric) water, data on changes in the wetness of past mire surfaces could even be treated as data on past climates. Different factors shaping the process of peat decomposition are also discussed, such as humidity of the substratum and climatic conditions, as well as the chemical composition of peat-forming plants. Methods for the determination of the degree of peat decomposition are also outlined, maintaining the division into field and laboratory analyses. Among the latter are methods based on physical and chemical features of peat and microscopic methods. Comparisons of results obtained by different methods can occasionally be difficult, which may be ascribed to different experience of researchers or the chemically undefined nature of many analyses of humification.

  19. Calibration of Local Area Weather Radar—Identifying significant factors affecting the calibration

    Science.gov (United States)

    Pedersen, Lisbeth; Jensen, Niels Einar; Madsen, Henrik

    2010-07-01

    A Local Area Weather Radar (LAWR) is an X-band weather radar developed to meet the needs of high resolution rainfall data for hydrological applications. The LAWR system and data processing methods are reviewed in the first part of this paper, while the second part of the paper focuses on calibration. The data processing for handling the partial beam filling issue was found to be essential to the calibration. LAWR uses a different calibration process compared to conventional weather radars, which use a power-law relationship between reflectivity and rainfall rate. Instead LAWR uses a linear relationship of reflectivity and rainfall rate as result of the log transformation carried out by the logarithmic receiver as opposed to the linear receiver of conventional weather radars. Based on rain gauge data for a five month period from a dense network of nine gauges within a 500 × 500 m area and data from a nearby LAWR, the existing calibration method was tested and two new methods were developed. The three calibration methods were verified with three external gauges placed in different locations. It can be concluded that the LAWR calibration uncertainties can be reduced by 50% in two out of three cases when the calibration is based on a factorized 3 parameter linear model instead of a single parameter linear model.

  20. Commonly studied polymorphisms in inflammatory cytokine genes show only minor effects on mortality and related risk factors in nonagenarians.

    Science.gov (United States)

    Dato, Serena; Krabbe, Karen S; Thinggaard, Mikael; Pedersen, Bente K; Christensen, Kaare; Bruunsgaard, Helle; Christiansen, Lene

    2010-03-01

    Systemic low-grade inflammation is consistently associated with functional status, cognitive functioning, multimorbidity, and survival in oldest olds. If inflammation is either a cause or a consequence of age-related pathology, genetic determinants of late-life survival can reside in cytokine genes polymorphisms, regulating inflammatory responses. The aim of this study was to test associations between commonly studied polymorphisms in interleukin (IL)6, IL10, IL15, and IL18, and tumor necrosis factor-alpha genes and late-life survival in a longitudinal cohort of nonagenarians: the Danish 1905 cohort. Additionally, associations were investigated between inflammatory markers and major predictors of mortality as cognitive and functional status. Modest sex-specific associations were found with survival, cognitive functioning, and handgrip strength. Evaluation of combined genotypes indicated that, in nonagenarian men, the balance of pro- and anti-inflammatory activity at IL18 and IL10 loci is protective against cognitive decline. In conclusion, in this large study with virtually complete follow-up, commonly studied polymorphisms in cytokine genes do not have a major impact on late-life survival or associated risk phenotypes.

  1. Field Studies Show That In Situ Greenhouse Gas Emission Factors for East African Agriculture Are Less Than IPCC Values

    Science.gov (United States)

    Pelster, D.; Butterbach-Bahl, K.; Rufino, M.; Rosenstock, T. S.; Wanyama, G.

    2015-12-01

    Greenhouse gas (GHG) emissions from African agricultural systems are thought to comprise a large portion of total emissions from the continent, however these estimates have been calculated using emission factors (EF) from other regions due to the lack of field studies in Africa, which results in large uncertainties for these estimates. Field measurements from western Kenya calculating emissions over a year in 59 different sites found that GHG emissions from typical smallholder farms ranged from 2.8 to 15.0 Mg CO2-C ha-1, -6.0 to 2.4 kg CH4-C ha-1 and -0.1 to 1.8 kg N2O-N ha-1, and were not affected by management intensity. The lack of a response in N2O emissions to N fertilization suggests that the EF currently used in national inventories overestimates N2O emissions from typical smallholder agriculture. Another study measuring N2O and CH4 emissions from manure deposited by grazing cattle found that the N2O EF ranged from 0.1 to 0.2%, while the CH4 EF ranged from 0.04 to 0.14 Kg CH4-C per 173 kg animal. These suggest that the current IPCC EF overestimate agricultural soil and manure GHG emissions for Kenya, and likely for much of East Africa.

  2. Changes and significances of islet β-cell function, oxidative stress and adipocyte factor in gestational diabetes mellitus

    Institute of Scientific and Technical Information of China (English)

    Guang-Yu Sun

    2016-01-01

    Objective:To investigate the changes and significances of islet β-cell function, oxidative stress and adipocyte factor in gestational diabetes mellitusthe. Methods:A total of 60 cases of gestational diabetes mellitus (GDM) patients were regarded as GDM group, a total of 60 cases of normal pregnant women were regarded as pregnant group, and a total of 60 cases of healthy women were regarded as control group. Isletβ-cell function, oxidative stress and adipocyte factor were measured and compared in the three groups. Results:For isletβ-cell function, the levels of FBG, FINS and HOMA-IR in GDM group significantly increased and the levels of HOMA-β and ISI in GDM group significantly decreased compared with control group and pregnant group. For oxidative stress, the level of MDA in GDM group significantly increased and the levels of SOD, GSH and TAOC in GDM group significantly decreased compared with control group and pregnant group. For adipocyte factor, the levels of adiponectin and visfatin in GDM group significantly decreased and the levels of leptin and resistin in GDM group significantly increased compared with control group and pregnant group. Conclusion:Gestational diabetes mellitus could result in impairment of islet β-cell function, decrease of insulin, oxidative stress and abnormality of adipocyte factor .

  3. Expression and significance of transforming growth factor β-activated kinase 1 in uterine myoma tissue

    Institute of Scientific and Technical Information of China (English)

    Xiao-Ping Chen; Li Zhao

    2016-01-01

    Objective:To compare the expression of TAK1 in normal myometrium and uterine leiomyoma tissue, and explore the relationship between these molecules and pathogenesis of uterine leiomyoma. Methods:Formaldehyde-fixed, paraffin-embedded specimen from 76 patients with uterine leiomyoma and normal myometrium adjacent were obtained from our hospital. The expression of TAK1 was detected by immunohistochemical staining and PCR analysis. Results:The expression of protein and mRNA levels TAK1 in uterine leiomyoma tissues were significantly increased when compared with normal myometrium, but not related to the number of leiomyoma. Furthermore, TAK1 expression was associated with the size of uterine leiomyoma. Conclusion:The present results suggest that the abundant expression of TAK1 protein may have a molecular basis characteristic of leiomyomas in the human uterus, and TAK1 may be a therapeutic target for the treatment of uterine leiomyoma.

  4. Expression and Clinical Significance of Vascular Endothelial Growth Factor in Benign and Malignant Tissues of Breast

    Institute of Scientific and Technical Information of China (English)

    XU Wei-gu; LIU Yang; WANG Gang; SONG Ji-ning; YANG Xiao-qing; WANG Wen-ya

    2008-01-01

    Objective:To detect the expression of vascular endothelial growth factor(VEGF)and microvessel density(MVD)count in breast benign affection,breast atypical hyperplasia and breast carcinoma in situ,and to clarify the relationship between VEGF expression,MVD and the clinicopathological features of these diseases. Methods:The expression of VEGF and MVD count in 115 cases breast benign diseases(including 40 breast fibroid tumor,40 breast cystic hyperplasia and 35 intraductal papilloma,19 breast atypical hyperplasias and 32 breast carcinomas in situ were examined by immunohistochemistry staining(SP-method). Results:The positive rate of VEGF in breast benign diseases,breast atypical hyperplasia and breast carcinoma in situ were 21.74%(25/115)、31.58.%(6/19)and 53.13%(17/32)respectively.It was the lowest in breast benign affection group,and was the highest breast carcinoma in situ group.The expression of VEGF increased gradually in the three groups(P<0.05).The MVD count of the three groups were 14.41±2.59,18.89±4.47 and 21.13±4.12 respectively,It was the lowest in breast benign affection group,and was the highest breast carcinoma in situ group.The MVD count of the three groups increased gradually(P<0.05).In VEGF positive group,MVD count was 19.41±4.78;In VEGF negative group,MVD count was 14.91±3.15.The MVD count was higher in VEGF positive group than that in VEGF negative group(P<0.05). Conclusion:The results of this study suggested that VEGF could promote microvessel growth in breast tumors.The occurrence and progression of breast cancer might be related with the expression of VEGF.

  5. Flow cytometric detection of growth factor receptors in autografts and analysis of growth factor concentrations in autologous stem cell transplantation: possible significance for platelet recovery

    DEFF Research Database (Denmark)

    Schiødt, I; Jensen, Charlotte Harken; Kjaersgaard, E

    2000-01-01

    In order to improve prediction of hematopoietic recovery, we conducted a pilot study, analyzing the significance of growth factor receptor expression in autografts as well as endogenous growth factor levels in blood before, during and after stem cell transplantation. Three early acting (stem cell...

  6. Show Time

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    <正> Story: Show Time!The whole class presents the story"Under the Sea".Everyone is so excited and happy.Both Leo and Kathy show their parentsthe characters of the play."Who’s he?"asks Kathy’s mom."He’s the prince."Kathy replies."Who’s she?"asks Leo’s dad."She’s the queen."Leo replieswith a smile.

  7. Snobbish Show

    Institute of Scientific and Technical Information of China (English)

    YIN PUMIN

    2010-01-01

    @@ The State Administration of Radio,Film and Television (SARFT),China's media watchdog,issued a new set of mles on June 9 that strictly regulate TV match-making shows,which have been sweeping the country's primetime programming. "Improper social and love values such as money worship should not be presented in these shows.Humiliation,verbal attacks and sex-implied vulgar content are not allowed" the new roles said.

  8. Determination of Serum Interleukin-13 and Nerve Growth Factor in Patients with Systemic Lupus Erythematosus and Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    XU Zusen; CHEN Yanlei

    2005-01-01

    Summary: The changes in the levels of serum interleukin-13 (IL-13) and nerve growth factor (NGF) in patients with systemic lupus erythematosus (SLE) and their clinical significance were investigated. Sandwich ELISA was used to determine the levels of serum IL-13 and NGF in 35 SLE patients and 15 normal controls. The results showed that the levels of serum IL-13 (92.69±9.87 pg/ml) and NGF (339.69±25.60 pg/ml) in active SLE patients were significantly higher than those in inactive SLE patients (IL-13, 54.22±9.31 pg/ml; NGF, 300.89±33.51 pg/ml)(P<0.01). The inactive patients also had significantly increased serum levels of IL-13 and NGF as compared with normal controls (IL-13, 35.20±12.70 pg/ml; NGF, 111.40±32.54 pg/ml; P<0.05 and P<0.01, respectively). Spearman correlation analysis revealed that the serum IL-13 levels were correlated with disease activity index of SLE (SLEDAI), ESR and serum levels of C3 (r= 0.813, 0.504, -0.605, respectively). The serum NGF levels were also correlated with above markers (r=0.442, 0.338, -0.463, respectively). The serum levels of IL-13 and NGF had a positive correlation (r=0.506, P<0.01). It was suggested that IL-13 and NGF might be involved in the pathogenesis of SLE and closely correlated with disease activity.

  9. Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight.

    Directory of Open Access Journals (Sweden)

    Charalambos Demetriou

    Full Text Available CONTEXT: Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight. OBJECTIVE: The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight. DESIGN: Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS (n = 260 collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors. RESULTS: Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively. No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7. INTERPRETATION: Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth.

  10. Prevalence of conventional risk factors and lipid profiles in patients with acute coronary syndrome and significant coronary disease

    Science.gov (United States)

    González-Pacheco, Héctor; Vargas-Barrón, Jesús; Vallejo, Maite; Piña-Reyna, Yigal; Altamirano-Castillo, Alfredo; Sánchez-Tapia, Pedro; Martínez-Sánchez, Carlos

    2014-01-01

    Background Among patients with coronary artery disease (CAD), 80%–90% present at least one conventional risk factor. On the other hand, lipid profile modification after a cardiovascular event related to acute coronary syndrome (ACS) has been recognized. The prevalence of conventional risk factors and the lipid profile at the time of admission in patients with ACS and significant CAD (stenosis ≥50%) determined through coronary angiography is not well described. Methods We studied 3,447 patients with a diagnosis of ACS and significant CAD with stenosis ≥50%, as shown o n angiography. We recorded the presence of conventional risk factors, including smoking, hypertension, dyslipidemia, and diabetes. In addition, we analyzed the lipid profiles within the first 24 hours of admission. We analyzed the studied population and compared findings according to sex. Results Most patients (81.7%) were male. ST-elevation myocardial infarction was present in 51.3% of patients, and non-ST-elevation acute coronary syndrome was present in 48.7%. The most frequent risk factor was smoking, which was present in 68% of patients, followed by hypertension (57.8%), dyslipidemia (47.5%), and diabetes (37.7%). In women, the most frequent risk factors were hypertension, diabetes, and dyslipidemia, whereas in men, smoking was the most frequent. We identified at least one risk factor in 95.7% of all patients, two or three risk factors in 62%, and four risk factors in 8.6% of patients. The lipid profile analysis revealed that 85.1% of patients had some type of dyslipidemia, and the most frequent was low levels of high-density lipoprotein cholesterol (68.6% of cases). Conclusion We found at least one conventional risk factor in 95.7% of patients with ACS and significant CAD. The lipid profile analysis revealed that two thirds of cases had low high-density lipoprotein cholesterol levels. PMID:25328397

  11. EROBATIC SHOW

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    Visitors look at plane models of the Commercial Aircraft Corp. of China, developer of the count,s first homegrown large passenger jet C919, during the Singapore Airshow on February 16. The biennial event is the largest airshow in Asia and one of the most important aviation and defense shows worldwide. A number of Chinese companies took part in the event during which Okay Airways, the first privately owned aidine in China, signed a deal to acquire 12 Boeing 737 jets.

  12. Risk stratification in multiple myeloma, part 2: the significance of genetic risk factors in the era of currently available therapies.

    Science.gov (United States)

    Biran, Noa; Jagannath, Sundar; Chari, Ajai

    2013-01-01

    Multiple myeloma (MM) is a heterogeneous disease, and a variety of risk factors at the time of initial diagnosis can be used to stratify patients. In the first part of this 2-part series, we reviewed the currently identified prognostic factors, characterized by disease burden, host factors, tumor biology, and depth of response to therapy. However, these risk factors cannot be interpreted independently of therapies. Novel therapies have the potential to worsen or improve outcomes compared with conventional therapy in high-risk patients, or actually overcome the high-risk status, thereby resulting in reclassification as standard risk. For example, thalidomide (Thalomid, Celgene) is associated with worse outcomes in patients with high-risk cytogenetic abnormalities, such as deletion of chromosomes 13 and 17p, whereas proteasome inhibitors appear to overcome t(4;14). The second part of this series reviews the significance of various genetic risks in the era of novel therapies for MM.

  13. Prevalence of conventional risk factors and lipid profiles in patients with acute coronary syndrome and significant coronary disease

    Directory of Open Access Journals (Sweden)

    González-Pacheco H

    2014-10-01

    Full Text Available Héctor González-Pacheco,1 Jesús Vargas-Barrón,2 Maite Vallejo,2 Yigal Piña-Reyna,3 Alfredo Altamirano-Castillo,1 Pedro Sánchez-Tapia,1 Carlos Martínez-Sánchez1 1Coronary Care Unit, National Institute of Cardiology in Mexico City, Mexico City, Mexico; 2Department of Clinical Research, National Institute of Cardiology in Mexico City, Mexico City, Mexico; 3Catheterization Laboratory, National Institute of Cardiology in Mexico City, Mexico City, Mexico Background: Among patients with coronary artery disease (CAD, 80%–90% present at least one conventional risk factor. On the other hand, lipid profile modification after a cardiovascular event related to acute coronary syndrome (ACS has been recognized. The prevalence of conventional risk factors and the lipid profile at the time of admission in patients with ACS and significant CAD (stenosis ≥50% determined through coronary angiography is not well described. Methods: We studied 3,447 patients with a diagnosis of ACS and significant CAD with stenosis ≥50%, as shown on angiography. We recorded the presence of conventional risk factors, including smoking, hypertension, dyslipidemia, and diabetes. In addition, we analyzed the lipid profiles within the first 24 hours of admission. We analyzed the studied population and compared findings according to sex.Results: Most patients (81.7% were male. ST-elevation myocardial infarction was present in 51.3% of patients, and non-ST-elevation acute coronary syndrome was present in 48.7%. The most frequent risk factor was smoking, which was present in 68% of patients, followed by hypertension (57.8%, dyslipidemia (47.5%, and diabetes (37.7%. In women, the most frequent risk factors were hypertension, diabetes, and dyslipidemia, whereas in men, smoking was the most frequent. We identified at least one risk factor in 95.7% of all patients, two or three risk factors in 62%, and four risk factors in 8.6% of patients. The lipid profile analysis revealed that

  14. An integrated model of transcription factor diffusion shows the importance of intersegmental transfer and quaternary protein structure for target site finding.

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    Hugo G Schmidt

    Full Text Available We present a computational model of transcription factor motion that explains both the observed rapid target finding of transcription factors, and how this motion influences protein and genome structure. Using the Smoldyn software, we modelled transcription factor motion arising from a combination of unrestricted 3D diffusion in the nucleoplasm, sliding along the DNA filament, and transferring directly between filament sections by intersegmental transfer. This presents a fine-grain picture of the way in which transcription factors find their targets two orders of magnitude faster than 3D diffusion alone allows. Eukaryotic genomes contain sections of nucleosome free regions (NFRs around the promoters; our model shows that the presence and size of these NFRs can be explained as their acting as antennas on which transcription factors slide to reach their targets. Additionally, our model shows that intersegmental transfer may have shaped the quaternary structure of transcription factors: sequence specific DNA binding proteins are unusually enriched in dimers and tetramers, perhaps because these allow intersegmental transfer, which accelerates target site finding. Finally, our model shows that a 'hopping' motion can emerge from 3D diffusion on small scales. This explains the apparently long sliding lengths that have been observed for some DNA binding proteins observed in vitro. Together, these results suggest that transcription factor diffusion dynamics help drive the evolution of protein and genome structure.

  15. Clinically significant fatigue: prevalence and associated factors in an international sample of adults with multiple sclerosis recruited via the internet.

    Directory of Open Access Journals (Sweden)

    Tracey J Weiland

    Full Text Available Fatigue contributes a significant burden of disease for people with multiple sclerosis (PwMS. Modifiable lifestyle factors have been recognized as having a role in a range of morbidity outcomes in PwMS. There is significant potential to prevent and treat fatigue in PwMS by addressing modifiable risk factors.To explore the associations between clinically significant fatigue and demographic factors, clinical factors (health-related quality of life, disability and relapse rate and modifiable lifestyle, disease-modifying drugs (DMD and supplement use in a large international sample of PwMS.PwMS were recruited to the study via Web 2.0 platforms and completed a comprehensive survey measuring demographic, lifestyle and clinical characteristics, including health-related quality of life, disability, and relapse rate.Of 2469 participants with confirmed MS, 2138 (86.6% completed a validated measure of clinically significant fatigue, the Fatigue Severity Scale. Participants were predominantly female from English speaking countries, with relatively high levels of education, and due to recruitment methods may have been highly pro-active about engaging in lifestyle management and self-help. Approximately two thirds of our sample (1402/2138; 65.6% (95% CI 63.7-67.7 screened positive for clinically significant fatigue. Bivariate associations were present between clinically significant fatigue and several demographic, clinical, lifestyle, and medication variables. After controlling for level of disability and a range of stable socio-demographic variables, we found increased odds of fatigue associated with obesity, DMD use, poor diet, and reduced odds of fatigue with exercise, fish consumption, moderate alcohol use, and supplementation with vitamin D and flaxseed oil.This study supports strong and significant associations between clinically significant fatigue and modifiable lifestyle factors. Longitudinal follow-up of this sample may help clarify the contribution

  16. Hip dysplasia: a significant risk factor for the development of hip osteoarthritis. A cross-sectional survey

    DEFF Research Database (Denmark)

    Jacobsen, Steffen; Sonne-Holm, Stig

    2005-01-01

    joint space width (JSW) /=60 yr of age. Of factors entered into logistic regression analyses, only age (P LT 0.001 for right hips and P LT 0.001 for left hips) and hip dysplasia (P LT 0.001 for right hips and P = 0.004 for left hips) were significantly associated with hip OA prevalence in women. In men......, only hip dysplasia was associated with hip OA prevalence, P LT 0.001 in right hips and P = 0.001 in left hips. CONCLUSIONS: Of the individual risk factors investigated in this study, only age and hip dysplasia were associated with the development of hip osteoarthritis.......OBJECTIVES: The aim of this cross-sectional survey of 2232 women and 1336 men (age range 20-91 yr) was to investigate individual risk factors for hip joint osteoarthritis (OA). METHODS: Standardized, weight-bearing pelvic radiographs were evaluated. Radiological hip joint OA was defined as minimum...

  17. Significant factors in the technological profile of innovative companies in the return of capital in technological innovation

    Directory of Open Access Journals (Sweden)

    Eloisa de Moura Lopes

    2013-12-01

    Full Text Available  Aim to analyze the significance of factors of technological profile in innovative companies that contribute to the return of capital in innovation (ROEI. The method used was design of experiments, by the structure of non-balanced factorial design. The field research was done with seventy companies. The main return of the capital applied in innovation, should support the open innovation system, and exclusively systematic studies of ideas for new products.  

  18. Mouse Dac, a novel nuclear factor with homology to Drosophila dachshund shows a dynamic expression in the neural crest, the eye, the neocortex, and the limb bud.

    Science.gov (United States)

    Caubit, X; Thangarajah, R; Theil, T; Wirth, J; Nothwang, H G; Rüther, U; Krauss, S

    1999-01-01

    Dac is a novel nuclear factor in mouse and humans that shares homology with Drosophila dachshund (dac). Alignment with available sequences defines a conserved box of 117 amino acids that shares weak homology with the proto-oncogene Ski and Sno. Dac expression is found in various neuroectodermal and mesenchymal tissues. At early developmental stages Dac is expressed in lateral mesoderm and in neural crest cells. In the neural plate/tube Dac expression is initially seen in the prosencephalon and gets gradually restricted to the presumptive neocortex and the distal portion of the outgrowing optic vesicle. Furthermore, Dac transcripts are detected in the mesenchyme underlying the Apical Ectodermal Ridge (AER) of the extending limb bud, the dorsal root ganglia and chain ganglia, and the mesenchyme of the growing genitalia. Dac expression in the Gli 3 mutant extra toes (Xt/Xt) shows little difference compared to the expression in wild-type limb buds. In contrast, a significant expansion of Dac expression are observed in the anterior mesenchyme of the limb buds of hemimelic extra toes (Hx/+) mice. FISH analysis reveals that human DAC maps to chromosome 13q22.3-23 and further fine-mapping defined a position of the DAC gene at 54cM or 13q21.1, a locus that associates with mental retardation and skeletal abnormalities.

  19. Comparison of the levels of the growth factors in umbilical cord serum and human milk and its clinical significance.

    Science.gov (United States)

    Patki, Satish; Patki, Ujjwala; Patil, Rajendra; Indumathi, S; Kaingade, Pankaj; Bulbule, Akshata; Nikam, Amar; Pishte, Amit

    2012-08-01

    The process of the growth of the fetus begins in the uterus and gets further accelerated following the birth, especially during initial few months. The role of the growth factors in the physiology of the cellular growth is already well established. Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) seem to be imperative for angiogenesis, cell development and proliferation as well as maintenance of the tissues. The levels of these factors in the maternal serum during pregnancy as well as during postpartum period are insignificant. Consequently, we hypothesized that the fetus receives moderate supply of these growth factors from the placenta during its stay in the uterus. This supply gets further augmented during the postpartum period through the different source, i.e. mother's milk. To study this physiological transition of the source of the growth factors from the placenta to the breast milk, the concentrations of VEGF and HGF in the cord serum of full term neonates and that in the breast milk of the corresponding mothers were analyzed during ELISA. The human milk, especially the colostrum revealed significantly higher levels of VEGF and HGF (1541.759 ± 119.349 pg/ml and 7129.249 ± 273.472 pg/ml) than cord serum (16.632 ± 0.773 pg/ml and 2581.6 ± 108.275 pg/ml) respectively. The multifold higher levels of VEGF observed in colostrum probably correlates with its high neonatal requirement for the maturation of the gastrointestinal epithelium following birth. The higher levels of both the growth factors in the breast milk than those observed in the cord serum probably explain their higher needs by the neonates for immunological protection, protein synthesis and neurocognitive development. The observations of the present study strengthen the policy of the colostrum feeding, which is promoted by organizations like World Health Organization (WHO). This study further documents the fact that the commercial milk formulae cannot replace the human

  20. A unique modification of the eukaryotic initiation factor 5A shows the presence of the complete hypusine pathway in Leishmania donovani.

    Directory of Open Access Journals (Sweden)

    Bhavna Chawla

    Full Text Available Deoxyhypusine hydroxylase (DOHH catalyzes the final step in the post-translational synthesis of an unusual amino acid hypusine (N(€-(4-amino-2-hydroxybutyl lysine, which is present on only one cellular protein, eukaryotic initiation factor 5A (eIF5A. We present here the molecular and structural basis of the function of DOHH from the protozoan parasite, Leishmania donovani, which causes visceral leishmaniasis. The L. donovani DOHH gene is 981 bp and encodes a putative polypeptide of 326 amino acids. DOHH is a HEAT-repeat protein with eight tandem repeats of α-helical pairs. Four conserved histidine-glutamate sequences have been identified that may act as metal coordination sites. A ~42 kDa recombinant protein with a His-tag was obtained by heterologous expression of DOHH in Escherichia coli. Purified recombinant DOHH effectively catalyzed the hydroxylation of the intermediate, eIF5A-deoxyhypusine (eIF5A-Dhp, in vitro. L. donovani DOHH (LdDOHH showed ~40.6% sequence identity with its human homolog. The alignment of L. donovani DOHH with the human homolog shows that there are two significant insertions in the former, corresponding to the alignment positions 159-162 (four amino acid residues and 174-183 (ten amino acid residues which are present in the variable loop connecting the N- and C-terminal halves of the protein, the latter being present near the substrate binding site. Deletion of the ten-amino-acid-long insertion decreased LdDOHH activity to 14% of the wild type recombinant LdDOHH. Metal chelators like ciclopirox olamine (CPX and mimosine significantly inhibited the growth of L. donovani and DOHH activity in vitro. These inhibitors were more effective against the parasite enzyme than the human enzyme. This report, for the first time, confirms the presence of a complete hypusine pathway in a kinetoplastid unlike eubacteria and archaea. The structural differences between the L. donovani DOHH and the human homolog may be exploited for

  1. A unique modification of the eukaryotic initiation factor 5A shows the presence of the complete hypusine pathway in Leishmania donovani.

    Science.gov (United States)

    Chawla, Bhavna; Kumar, Ravi Ranjan; Tyagi, Nidhi; Subramanian, Gowri; Srinivasan, N; Park, Myung Hee; Madhubala, Rentala

    2012-01-01

    Deoxyhypusine hydroxylase (DOHH) catalyzes the final step in the post-translational synthesis of an unusual amino acid hypusine (N(€)-(4-amino-2-hydroxybutyl) lysine), which is present on only one cellular protein, eukaryotic initiation factor 5A (eIF5A). We present here the molecular and structural basis of the function of DOHH from the protozoan parasite, Leishmania donovani, which causes visceral leishmaniasis. The L. donovani DOHH gene is 981 bp and encodes a putative polypeptide of 326 amino acids. DOHH is a HEAT-repeat protein with eight tandem repeats of α-helical pairs. Four conserved histidine-glutamate sequences have been identified that may act as metal coordination sites. A ~42 kDa recombinant protein with a His-tag was obtained by heterologous expression of DOHH in Escherichia coli. Purified recombinant DOHH effectively catalyzed the hydroxylation of the intermediate, eIF5A-deoxyhypusine (eIF5A-Dhp), in vitro. L. donovani DOHH (LdDOHH) showed ~40.6% sequence identity with its human homolog. The alignment of L. donovani DOHH with the human homolog shows that there are two significant insertions in the former, corresponding to the alignment positions 159-162 (four amino acid residues) and 174-183 (ten amino acid residues) which are present in the variable loop connecting the N- and C-terminal halves of the protein, the latter being present near the substrate binding site. Deletion of the ten-amino-acid-long insertion decreased LdDOHH activity to 14% of the wild type recombinant LdDOHH. Metal chelators like ciclopirox olamine (CPX) and mimosine significantly inhibited the growth of L. donovani and DOHH activity in vitro. These inhibitors were more effective against the parasite enzyme than the human enzyme. This report, for the first time, confirms the presence of a complete hypusine pathway in a kinetoplastid unlike eubacteria and archaea. The structural differences between the L. donovani DOHH and the human homolog may be exploited for structure

  2. Children born extremely preterm show significant lower cognitive, language and motor function levels compared with children born at term, as measured by the Bayley-III at 2.5 years.

    Science.gov (United States)

    Månsson, J; Stjernqvist, K

    2014-05-01

    To assess developmental outcomes of children aged 2.5 years born extremely preterm. As a part of the population-based Extremely Preterm Infants in Sweden Study (EXPRESS), 399 children born before 27 weeks of gestation and 366 control children born at term were assessed with the Bayley Scales of Infant and Toddler Development, third edition (Bayley-III), assigning scores for cognition, receptive and expressive communication, fine and gross motor functions. Based on control group means, prevalences of developmental delay in the preterm group were calculated. Mean score differences between subtests constituting the overall Bayley-III indices were analysed within both groups. After controlling for socio-demographic, child and assessment variables, analyses showed significantly lower performances of the preterm group compared with the control group on the Bayley-III subtests. Prevalence of moderate-severe delay was 10.8% in cognitive, 14.9% in receptive communication, 14.5% in expressive communication, 12.4% in fine motor and 7.0% in gross motor functions. Significant differences between performances on subtests included in the same indices were detected. Extremely preterm children show significant lower cognitive, communicative and motor function levels at 2.5 years compared with children born at term. Bayley-III assessments permit the acquisition of nuanced information about development following extreme prematurity. ©2014 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.

  3. Expression and significance of myeloid differentiation factor 88 in marrow dendritic cells in asthmatic rats with cigarette smoke exposure

    Institute of Scientific and Technical Information of China (English)

    LI Yi; DU Yong-cheng; XU Jian-ying; HU Xiao-yun

    2012-01-01

    Background Smoking causes frequent asthma attacks,leading to a rapid decline in lung function in patients with asthma,and it can also reduce the therapeutic effect of glucocorticeids in patients with asthma.Therefore,the present study aimed to investigate the effect of cigarette smoke on the expression of myeloid differentiation factor 88 (MyD88) in marrow dendritic cells (DCs) in asthmatic rats,and to explore the molecular mechanism of cigarette smoke exposure on asthma by DCs.Methods Forty Wistar rats were randomly divided into the following groups:control,smoke exposure,asthma,and asthma combined with smoke exposure.The animal model was established,and then rat bone marrow-derived DCs were collected.Additionally,rat spleen lymphocytes and bone marrow-derived DCs were cultured together for mixed lymphocyte responses.Interferon (IFN)-gamma and interleukin (IL)-4,IL-10,and IL-12 expressions were determined by enzyme-linked immunosorbent assay (ELISA).MyD88 expression was determined by Western blotting.The proliferation of lymphocytes was examined with methyl thiazolyl tetrazolium (MTT) colorimetric assay.Results MyD88 expression was decreased in the asthma combined with smoke exposure group compared to the asthma group (P<0.01),and IL-10 and IL-12 expressions were decreased in the asthma combined with smoke exposure group compared to control group (P<0.01).In addition,DCs stimulating activity on allogeneic lymphocytes were significantly decreased in the smoke exposure combined with asthma group compared to the control and asthma groups (P<0.01).After allogeneic mixed lymphocyte responses,IL-4 expression was increased and IFN-gamma was decreased in the asthma group and the asthma combined with smoke exposure group compared to control group (P<0.01).IL-4expression was increased and IFN-gamma was decreased in the asthma combined with smoke exposure group compared to the asthma group (P<0.01).The study also showed that MyD88 expression was positively

  4. The clinical significance of tumor necrosis factor-alpha plasma level in patients having chronic lymphocytic leukemia.

    Science.gov (United States)

    Ferrajoli, Alessandra; Keating, Michael J; Manshouri, Taghi; Giles, Francis J; Dey, Amanda; Estrov, Zeev; Koller, Charles A; Kurzrock, Razelle; Thomas, Deborah A; Faderl, Stefan; Lerner, Susan; O'Brien, Susan; Albitar, Maher

    2002-08-15

    Tumor necrosis factor-alpha (TNF-alpha), a cytokine possessing pleiotropic biological activities, is produced by leukemic lymphocytes in patients with chronic lymphocytic leukemia (CLL) and acts as an autocrine and paracrine growth factor in this disease. In this study, TNF-alpha levels were determined in 150 patients with CLL and correlated with disease characteristics, prognostic factors, and survival. The mean TNF-alpha plasma concentration in the patients with CLL was significantly higher than in the healthy control population (16.4 versus 8.7 pg/mL; P <.0001). Patients having an elevated TNF-alpha level had more advanced Rai and Binet stage disease, higher serum beta(2)-microglobulin (beta(2)M) levels, a greater percentage of cells expressing CD38, and lower hemoglobin and platelet levels. Patients having chromosomal abnormalities such as 11q deletion, trisomy 12, and chromosome 17 aberrations had a higher mean TNF-alpha level (27.5 pg/mL) than patients having a diploid karyotype or other miscellaneous cytogenetic abnormalities (14.2 pg/mL; P <.001). The TNF-alpha level was a predictor of survival when the Cox proportional hazards model was used with TNF-alpha entered as a continuous variable (P =.0001). Also, patients having a TNF-alpha level above the mean value of 14 pg/mL had significantly shorter survival duration (P =.00001). The TNF-alpha level remained predictive of survival in Cox multivariate analysis independent of Rai staging and beta(2)M, hemoglobin, prior therapy, white cell count, and platelet level (P =.005). We conclude that the TNF-alpha level serves as a prognostic factor in patients with CLL and that inhibition of TNF-alpha in these patients could have therapeutic importance.

  5. Significance of specificity of Tinetti B-POMA test and fall risk factor in third age of life.

    Science.gov (United States)

    Avdić, Dijana; Pecar, Dzemal

    2006-02-01

    As for the third age, psychophysical abilities of humans gradually decrease, while the ability of adaptation to endogenous and exogenous burdens is going down. In 1987, "Harada" et al. (1) have found out that 9.5 million persons in USA have difficulties running daily activities, while 59% of them (which is 5.6 million) are older than 65 years in age. The study has encompassed 77 questioned persons of both sexes with their average age 71.73 +/- 5.63 (scope of 65-90 years in age), chosen by random sampling. Each patient has been questioned in his/her own home and familiar to great extent with the methodology and aims of the questionnaire. Percentage of questioned women was 64.94% (50 patients) while the percentage for men was 35.06% (27 patients). As for the value of risk factor score achieved conducting the questionnaire and B-POMA test, there are statistically significant differences between men and women, as well as between patients who fell and those who never did. As for the way of life (alone or in the community), there are no significant statistical differences. Average results gained through B-POMA test in this study are statistically significantly higher in men and patients who did not provide data about falling, while there was no statistically significant difference in the way of life. In relation to the percentage of maximum number of positive answers to particular questions, regarding gender, way of life and the data about falling, there were no statistically significant differences between the value of B-POMA test and the risk factor score (the questionnaire).

  6. STUDY ON THE CLINICAL-PATHOLOGICAL SIGNIFICANCE OF MICROVESSEL DENSITY AND VASCULAR ENDOTHILIAL GROWTH FACTOR EXPRESSION IN PRIMARY LIVER CANCER

    Institute of Scientific and Technical Information of China (English)

    XIA Jing-lin; YANG Bing-hui; YE Sheng-long; LIN Zhi-ying; MA Zeng-chen; ZHOU Xin-da; WU Zhi-quan; TANG Zhao-you

    1999-01-01

    Objective: To evaluate the clinical-pathological significance of intratumoral microvessel density (MVD) and Vascular Endothelial Growth Factor (VEGF) expression in primary liver cancer (PLC). Methods: A retrospective study from 63 postoperative patients all with small PLC (diameter ≤ 5 cm) was done. One group of 29 patients developed recurrence or metastasis within 2 years. The other group of 34 patients had no evidence of recurrence or metastasis within 2 years. Three sections were taken from each patient. One for H.E. staining, the other two for VEGF and Bio-UEA-Ⅰ immunohistochemical staining respectively.MVD was counted by endothelial cells, which were highlighted by Bio-UEA-Ⅰ. Results: The MVD of the recurrence (or metastasis) group (49.6±29.7) were significantly greater than the other group (22.7±28.2) (P<0.01); The VEGF positive rate of the recurrence group was 86.2% (25/29), the rate of the other group was 47.1% (16/34). The difference between the 2 groups was stafistically significant (P<0.01). The stage of the tumor, the positive rate of satellite nodules and the positive rate of the portal vein embolus were all significantly different between the 2 groups. Conclusion: Besides tumor stage, satellite nodule and portal vein embolus, the MVD and VEGF are also of prognostic significance.

  7. Event-related potentials to tones show differences between children with multiple risk factors for dyslexia and control children before the onset of formal reading instruction.

    Science.gov (United States)

    Hämäläinen, Jarmo A; Lohvansuu, Kaisa; Ervast, Leena; Leppänen, Paavo H T

    2015-02-01

    Multiple risk factors can affect the development of specific reading problems or dyslexia. In addition to the most prevalent and studied risk factor, phonological processing, auditory discrimination problems have also been found in children and adults with reading difficulties. The present study examined 37 children between the ages of 5 and 6, 11 of which had multiple risk factors for developing reading problems. The children participated in a passive oddball EEG experiment with sinusoidal sounds with changes in sound frequency, duration, or intensity. The responses to the standard stimuli showed a negative voltage shift in children at risk for reading problems compared to control children at 107-215 ms in frontocentral areas corresponding to P1 offset and N250 onset. Source analyses showed that the difference originated from the left and right auditory cortices. Additionally, the children at risk for reading problems had a larger late discriminative negativity (LDN) response in amplitude for sound frequency change than the control children. The amplitudes at the P1-N250 time window showed correlations to letter knowledge and phonological identification whereas the amplitudes at the LDN time window correlated with verbal short-term memory and rapid naming. These results support the view that problems in basic auditory processing abilities precede the onset of reading instruction and can act as one of the risk factors for dyslexia.

  8. Expression and clinical significance of CD73 and hypoxia-inducible factor-1α in gastric carcinoma.

    Science.gov (United States)

    Lu, Xiao-Xia; Chen, Yi-Tian; Feng, Bing; Mao, Xiao-Bei; Yu, Bo; Chu, Xiao-Yuan

    2013-03-28

    To investigate the expression of CD73 and hypoxia-inducible factor-1α (HIF-1α) in human gastric carcinoma, and explore their clinical significance and prognostic value. CD73 and HIF-1α expressions were detected by immunohistochemistry in consecutive sections of tissue samples from 68 gastric carcinoma patients. The peritumor tissues 2 cm away from the tumor were obtained and served as controls. The presence of CD73 and HIF-1α was analyzed by immunohistochemistry using the Envision technique. CD73 and HIF-1α expressions in gastric carcinoma were significantly higher than those in gastric mucosal tissues as control (P gastric carcinoma by both multivariate Cox regression analysis (P = 0.021) and receiver operating characteristic curves (P = 0.001). CD73 expression correlates closely with HIF-1α expression in gastric carcinoma. CD73 could be an independent prognostic indicator for gastric carcinoma.

  9. Contributory factors to the results of gravity-assisted pivot-shift test for anterior cruciate ligament injury: the significance of muscle torque around the knee.

    Science.gov (United States)

    Hiraoka, Hisatada; Yashiki, Motohisa; Sakai, Hiroya

    2008-03-01

    Gravity-assisted pivot-shift (GAPS) test is a newly advocated test for anterior cruciate ligament (ACL) injury. It induces anterolateral rotatory instability with valgus stress to the knee applied by gravitational force during patient's active knee motion. We investigated prospectively the relationships between the results of the GAPS test and the possible contributory factors and sought to clarify the determinant factors of the GAPS test. A total of 54 knee joints of 54 patients with unilateral ACL injury (29 males, 25 females, average 23.4 +/- 9.0 years old) were enrolled in this study and were divided into two groups, i.e., positive GAPS test group and negative GAPS test group. Muscle torque around the knee joints measured before surgery, configuration of the femoral condyle and tibial posterior slope angle measured on lateral radiograph, and other clinical factors were compared between the two groups using Mann-Whitney U test or chi-square test. According to the results of these analyses, factors having a statistically significant difference were additionally evaluated using multiple logistic regression analysis to reveal items with strong relevance to a positive GAPS test. The results of the multiple logistic regression analysis showed that the flexor/extensor peak torque ratio of contralateral uninjured knees and sex had a significant correlation with the results of the GAPS test. The relatively less flexor muscle torque compared with extensor muscle torque, and being a female patient were considered to be the determinant factors of a positive GAPS test.

  10. 从礼貌原则探寻访谈节目成功的因素%Explore the Factors of Successful Talk Show from the Politeness Principle

    Institute of Scientific and Technical Information of China (English)

    杨欣榕

    2014-01-01

    "A Date with Luyu"is a successful talk show. This article aims to find out the key factors of its success from the perspective of Politeness Principle.%《鲁豫有约》是一档成功的访谈节目,本文从礼貌原则角度分析这档访谈节目成功的因素。

  11. Barley plants over-expressing the NAC transcription factor gene HvNAC005 show stunting and delay in development combined with early senescence

    DEFF Research Database (Denmark)

    Christiansen, Michael W.; Matthewman, Colette; Podzimska-Sroka, Dagmara Agata;

    2016-01-01

    -expressing plants showed up-regulation of genes involved with secondary metabolism, hormone metabolism, stress, signalling, development, and transport. Up-regulation of senescence markers and hormone metabolism and signalling genes supports a role of HvNAC005 in the cross field of different hormone and signalling......The plant-specific NAC transcription factors have attracted particular attention because of their involvement in stress responses, senescence, and nutrient remobilization. The HvNAC005 gene of barley encodes a protein belonging to subgroup NAC-a6 of the NAC family. This study shows that HvNAC005...

  12. Clinical Significance of Tissue Factor and CD13 Double-Positive Microparticles in Sirs Patients with Trauma and Severe Sepsis.

    Science.gov (United States)

    Matsumoto, Hisatake; Yamakawa, Kazuma; Ogura, Hiroshi; Koh, Taichin; Matsumoto, Naoya; Shimazu, Takeshi

    2017-04-01

    Activated immune cells such as monocytes are key factors in systemic inflammatory response syndrome (SIRS) following trauma and sepsis. Activated monocytes induce almost all tissue factor (TF) expression contributing to inflammation and coagulation. TF and CD13 double-positive microparticles (TF/CD13MPs) are predominantly released from these activated monocytes. This study aimed to evaluate TF/CD13MPs and assess their usefulness as a biomarker of pathogenesis in early SIRS following trauma and sepsis. This prospective study comprising 24 trauma patients, 25 severe sepsis patients, and 23 healthy controls was conducted from November 2012 to February 2015. Blood samples were collected from patients within 24 h after injury and diagnosis of severe sepsis and from healthy controls. Numbers of TF/CD13MPs were measured by flow cytometry immediately thereafter. Injury Severity Score (ISS) and Acute Physiology and Chronic Health Evaluation (APACHE) II and Sequential Organ Failure Assessment (SOFA) scores were calculated at patient enrollment. APACHE II and SOFA scores and International Society of Thrombosis and Haemostasis (ISTH) overt disseminated intravascular coagulation (DIC) diagnostic criteria algorithm were calculated at the time of enrollment of severe sepsis patients. Numbers of TF/CD13MPs were significantly increased in both trauma and severe sepsis patients versus controls and correlated significantly with ISS and APACHE II score in trauma patients and with APACHE II and ISTH DIC scores in severe sepsis patients. Increased numbers of TF/CD13MPs correlated significantly with severities in the acute phase in trauma and severe sepsis patients, suggesting that TF/CD13MPs are important in the pathogenesis of early SIRS following trauma and sepsis.

  13. A candidate gene study of serotonergic pathway genes and pain relief during treatment with escitalopram in patients with neuropathic pain shows significant association to serotonin receptor2C (HTR2C)

    DEFF Research Database (Denmark)

    Brasch-Andersen, Charlotte; Møller, Malik U; Christiansen, Lene

    2011-01-01

    the association between polymorphisms in genes involved in the serotonergic pathway and the effect of escitalopram on peripheral neuropathic pain. METHODS: We genotyped 34 participants from a placebo-controlled trial of escitalopram in peripheral neuropathic pain for polymorphisms in five genes: the serotonin.......047), with 75% carrying the C allele being responders. The same tendency was seen in women. Similarly, carriership of the C allele at rs6318 was associated with better pain relief during treatment with escitalopram [odds ratio (OR) 15.5, p = 0.014)] Furthermore, there was a tendency of better relief...... with increasing number of short alleles for the 5-HTTLPR polymorphism of the serotonin transporter (OR 5.7, p = 0.057). None of the other polymorphisms showed a significant association with treatment response to escitalopram. CONCLUSION: This study indicates that variation in the HTR2C gene is associated...

  14. Expression of Vascular Endothelial Growth Factor and Cyclooxygenase-2 in Laryngeal Squamous Cell Carcinoma and Its significance

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    In order to study the expressions of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) in human laryngeal squamous cell carcinoma (LSCC) and its significance,the expression of VEGF mRNA and COX-2 mRNA in 62 cases of LSCC and 54 adjacent noncancerous laryngeal tissues and 9 normal human laryngeal mucous tissues was detected by using techniques of semi-quantitative RT-PCR. It was found that the expression level of VEGF and COX-2 mRNA was significantly increased in LSCC as compared with that in the normal human laryngeal mucous tissues (both P<0.01), and the expression level of VEGF and COX-2 mRNA were significantly increased in stage Ⅲ + Ⅳ tissues of LSCC as compared with the stage Ⅰ + Ⅱ tissues of LSCC (P <0. 01). There was a high positive correlation between VEGF and COX-2 expression in LSCC (r=0. 756,P<0.01). These data raise the possibility that VEGF and COX-2 may play key roles in the growth, invasion and metastasis of LSCC.

  15. The crystal structure of the streptococcal collagen-like protein 2 globular domain from invasive M3-type group A Streptococcus shows significant similarity to immunomodulatory HIV protein gp41.

    Science.gov (United States)

    Squeglia, Flavia; Bachert, Beth; De Simone, Alfonso; Lukomski, Slawomir; Berisio, Rita

    2014-02-21

    The arsenal of virulence factors deployed by streptococci includes streptococcal collagen-like (Scl) proteins. These proteins, which are characterized by a globular domain and a collagen-like domain, play key roles in host adhesion, host immune defense evasion, and biofilm formation. In this work, we demonstrate that the Scl2.3 protein is expressed on the surface of invasive M3-type strain MGAS315 of Streptococcus pyogenes. We report the crystal structure of Scl2.3 globular domain, the first of any Scl. This structure shows a novel fold among collagen trimerization domains of either bacterial or human origin. Despite there being low sequence identity, we observed that Scl2.3 globular domain structurally resembles the gp41 subunit of the envelope glycoprotein from human immunodeficiency virus type 1, an essential subunit for viral fusion to human T cells. We combined crystallographic data with modeling and molecular dynamics techniques to gather information on the entire lollipop-like Scl2.3 structure. Molecular dynamics data evidence a high flexibility of Scl2.3 with remarkable interdomain motions that are likely instrumental to the protein biological function in mediating adhesive or immune-modulatory functions in host-pathogen interactions. Altogether, our results provide molecular tools for the understanding of Scl-mediated streptococcal pathogenesis and important structural insights for the future design of small molecular inhibitors of streptococcal invasion.

  16. U.S. Volunteering in the Aftermath of the Great Recession: Were African Americans a Significant Factor?

    Directory of Open Access Journals (Sweden)

    Vernon B. Carter

    2016-05-01

    Full Text Available The Great Recession weakened U.S. families’ abilities to make charitable gifts. Although African Americans are generally especially hard hit by these types of economic crises, they have a long and distinctive history of volunteerism and mutual assistance. Consequently, the purpose of this study is to examine African American volunteering in nonprofit organizations in the aftermath of the 2008–2009 recession. Specifically, we examined race as well as other factors with the potential to influence volunteering in four categories of organizations: poverty organizations, senior service agencies, social action groups, and religious affiliated organizations. Using the Panel Study of Income Dynamics (PSID data, this secondary analysis produced significant findings regarding volunteerism among African Americans in these community-based organizations.

  17. Extrinsic factors significantly affect patterns of disease in free-ranging and captive cheetah (Acinonyx jubatus) populations.

    Science.gov (United States)

    Munson, Linda; Terio, Karen A; Worley, Michael; Jago, Mark; Bagot-Smith, Arthur; Marker, Laurie

    2005-07-01

    The cheetah (Acinonyx jubatus) has been considered a paradigm for disease vulnerability due to loss of genetic diversity. This species monomorphism has been suspected to be the basis for their general poor health and dwindling populations in captivity. North American and South African captive populations have high prevalences of hepatic veno-occlusive disease, glomerulosclerosis, gastritis, and systemic amyloidosis, diseases that are rare in other species. Unusually severe inflammatory reactions to common infectious agents have also been documented in captive cheetahs. The current study compared disease prevalences in free-ranging Namibian cheetahs with those in two captive populations of similar ages. The occurrence of diseases in the free-ranging population was determined from 49 necropsies and 27 gastric biopsies obtained between 1986 and 2003 and compared with prevalences in 147 North American and 80 South African captive cheetahs. Except for two cheetahs, the free-ranging population was in robust health with only mild lesions present, in contrast with significantly higher prevalences in the captive populations. Despite widespread heavy Helicobacter colonization in wild cheetahs, only 3% of the free-ranging population had moderate to severe gastritis, in contrast with 64% of captive cheetahs. No severe inflammatory reactions to viral infections were detected in the free-ranging animals. Because free-ranging Namibian cheetahs are as genetically impoverished as captive cheetahs, these findings caution against attributing loss of fitness solely to genetic factors and attest to the fundamental importance of extrinsic factors in wildlife health.

  18. Expression and Prognostic Significance of Human Epidermal Growth Factor Receptors 1 and 3 in Gastric and Esophageal Adenocarcinoma.

    Directory of Open Access Journals (Sweden)

    Charlotta Hedner

    Full Text Available Gastric and esophageal adenocarcinomas are major global cancer burdens. These cancer forms are characterized by a poor prognosis and a modest response to chemo- radio- and targeted treatment. Hence there is an obvious need for further enhanced diagnostic and treatment strategies. The aim of this study was to examine the expression and prognostic impact of human epidermal growth factor receptor 1 (HER1/EGFR and 3 (HER3, as well as the occurrence of EGFR and KRAS mutations in gastric and esophageal adenocarcinoma.Immunohistochemical expression of EGFR and HER3 was analysed in all primary tumours and a subset of lymph node metastases in a consecutive cohort of 174 patients with adenocarcinoma of the stomach, cardia and esophagus. The anti-HER3 antibody used was validated by siRNA-mediated knockdown, immunohistochemistry and quantitative real-time PCR. EGFR and KRAS mutation status was analysed by pyrosequencing tecchnology.High EGFR expression was an independent risk factor for shorter overall survival (OS, whereas high HER3 expression was associated with a borderline significant trend towards a longer OS. KRAS mutations were present in only 4% of the tumours and had no prognostic impact. All tumours were EGFR wild-type. These findings contribute to the ongoing efforts to decide on the potential clinical value of different HERs and druggable mutations in gastric and esophageal adenocarcinomas, and attention is drawn to the need for more standardised investigational methods.

  19. Photocatalytic degradation kinetics of naphthenic acids in oil sands process-affected water: Multifactorial determination of significant factors.

    Science.gov (United States)

    Leshuk, Tim; de Oliveira Livera, Diogo; Peru, Kerry M; Headley, John V; Vijayaraghavan, Sucharita; Wong, Timothy; Gu, Frank

    2016-12-01

    Oil sands process-affected water (OSPW) is generated as a byproduct of bitumen extraction in Canada's oil sands. Due to the water's toxicity, associated with dissolved acid extractable organics (AEO), especially naphthenic acids (NAs), along with base-neutral organics, OSPW may require treatment to enable safe discharge to the environment. Heterogeneous photocatalysis is a promising advanced oxidation process (AOP) for OSPW remediation, however, predicting treatment efficacy can be challenging due to the unique water chemistry of OSPW from different tailings ponds. The objective of this work was to study various factors affecting the kinetics of photocatalytic AEO degradation in OSPW. The rate of photocatalytic treatment varied significantly in two different OSPW sources, which could not be accounted for by differences in AEO composition, as studied by high resolution mass spectrometry (HRMS). The effects of inorganic water constituents were investigated using factorial and response surface experiments, which revealed that hydroxyl (HO) radical scavenging by iron (Fe(3+)) and bicarbonate (HCO3(-)) inhibited the NA degradation rate. The effects of NA concentration and temperature on the treatment kinetics were also evaluated in terms of Langmuir-Hinshelwood and Arrhenius models; pH and temperature were identified as weak factors, while dissolved oxygen (DO) was critical to the photo-oxidation reaction. Accounting for all of these variables, a general empirical kinetic expression is proposed, enabling prediction of photocatalytic treatment performance in diverse sources of OSPW.

  20. Clinical significance of factor V leiden and prothrombin G20210A-mutations in cerebral venous thrombosis - comparison with arterial ischemic stroke.

    Science.gov (United States)

    Beye, Aida; Pindur, Gerhard

    2017-08-28

    Cerebrovascular diseases are considered in a different way concerning their etiology with regard to arterial and venous occlusion. The role of thrombophilia in this context remains undetermined. For this reason, a case-control study was conducted including a total of 202 patients (154 females, 48 males) aged from 18 to 76 years (mean: 39.8 years) suffering either from cerebral sinus venous thrombosis (n = 101) or from arterial ischemic stroke (n = 101). Study groups were evaluated on the basis of age- and gender-matched pairs. Gene mutations of factor V-1691 (factor V Leiden) and prothrombin-20210 being considered as the most common thrombophilia markers were analyzed in this study. Factor V Leiden-mutations were found in 16.8% of patients with cerebral sinus venous thrombosis (CVT) and in 17.8% of patients with arterial ischemic stroke (AIS), which was significantly more frequent than in controls at a rate of 4.95% (ORs: 3.89 and 4.16). Prothrombin-mutations were significantly more frequent in CVT at a rate of 14.9% versus 2.97% in controls (OR: 5.70). This does not apply for AIS showing a rate of 4.95% prothrombin-mutations. Rates of factor V Leiden-mutations are not different in CVT compared with AIS. In contrast, however, prothrombin-mutations were significantly more frequent in CVT than in AIS with a rate of 14.9% versus 4.95% (OR 3.35). Furthermore, 3 cases with combined heterozygosity of factor V Leiden- and prothrombin-mutation have been identified in CVT, but not in AIS or controls. All of the above mentioned mutations were exclusively heterozygous. We conclude from these data that thrombophilia in terms of factor V Leiden genotype is a risk factor for both CVT and AIS in equal measure. In contrast, prothrombin-20210-mutations were different playing a significant role in the pathogenesis of cerebral sinus vein- thrombosis, but not in arterial ischemic stroke. Also, the combined occurrence of heterozygous prothrombin- and factor V Leiden

  1. Preoperative Plasma Fibrinogen Level as a Significant Prognostic Factor in Patients With Localized Renal Cell Carcinoma After Surgical Treatment.

    Science.gov (United States)

    Lee, Hakmin; Lee, Sang Eun; Byun, Seok-Soo; Kim, Hyeon Hoe; Kwak, Cheol; Hong, Sung Kyu

    2016-01-01

    We sought to investigate the association of preoperative fibrinogen levels with clinicopathologic outcomes after surgical treatment of nonmetastatic renal cell carcinoma. We reviewed the records of 1511 patients who had their fibrinogen levels measured preceding surgery. The associations between preoperative fibrinogen level and risk of adverse clinicopathologic outcomes were tested using the multivariate logistic regression and multiple Cox-proportional hazards model, respectively. Based on plasma fibrinogen levels, we stratified the patients into 2 groups with a cut-off value of 328  mg/dL. Kaplan-Meier analysis showed significantly inferior survival outcomes in progression-free (P fibrinogen level (≥328  mg/dL) was significantly related to a higher Fuhrman grade (hazard ratio [HR] 1.374, P = 0.006) and a larger tumor size (≥7  cm) (HR 2.364, P fibrinogen level is a significant predictor for poor disease progression (HR 1.857, P fibrinogen levels were significantly associated with poor pathological features and worse survival outcomes in patients with nonmetastatic renal cell carcinoma after surgical treatment. Further evaluations such as prospective randomized trials are needed to understand the underlying mechanism for these associations.

  2. Members of the barley NAC transcription factor gene family show differential co-regulation with senescence-associated genes during senescence of flag leaves

    DEFF Research Database (Denmark)

    Christiansen, Michael W; Gregersen, Per L.

    2014-01-01

    The senescence process of plants is important for the completion of their life cycle, particularly for crop plants, it is essential for efficient nutrient remobilization during seed filling. It is a highly regulated process, and in order to address the regulatory aspect, the role of genes...... in the NAC transcription factor family during senescence of barley flag leaves was studied. Several members of the NAC transcription factor gene family were up-regulated during senescence in a microarray experiment, together with a large range of senescence-associated genes, reflecting the coordinated...... activation of degradation processes in senescing barley leaf tissues. This picture was confirmed in a detailed quantitative reverse transcription–PCR (qRT–PCR) experiment, which also showed distinct gene expression patterns for different members of the NAC gene family, suggesting a group of ~15 out of the 47...

  3. Clinical significance of serum levels of vascular endothelial growth factor and its receptor in biliary disease and carcinoma

    Institute of Scientific and Technical Information of China (English)

    Munechika Enjoji; Hajime Nawata; Makoto Nakamuta; Koji Yamaguchi; Kazuhiro Kotoh; Marie Fukushima; Masami Kuniyoshi; Masao Tanaka

    2005-01-01

    AIM: To investigate the clinical significance of serum vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (VEGFR1/Flt-1) (sVEGFR1) levels in biliary diseases.METHODS: We analyzed the serum levels of these proteins in patients with acute cholangitis (group 1), biliary malignancies (group 2), and primary biliary cirrhosis or primary sclerosing cholangitis (group 3), and in healthy donors (group 4). The influence of inflammation was also analyzed. Serum VEGF levels were expressed as VEGF per platelet (VEGF/PLT, pg/106) in order to exclude the influence of platelet counts.RESULTS: sVEGFR1 levels were significantly higher in groups 1 and 2 than in the control group, but did not correlate with inflammatory markers. VEGF/PLT levels were generally higher in patients with active inflammation than in those with carcinoma. C-reactive protein strongly correlated with the levels of serum VEGF independently of platelet and leukocyte counts, even in cancer patients. In cancer patients, VEGF/PLT and sVEGFR1 levels might be indicators for evaluating the effect of medical treatment or the disease progression.CONCLUSION: Serum VEGF and VEGFR1 might be useful markers for gauging the clinical effect of various treatments on patients.

  4. Expressions of cysteine-rich61, connective tissue growth factor and Nov genes in hepatocellular carcinoma and their clinical significance

    Institute of Scientific and Technical Information of China (English)

    Zhi-Jun Zeng; Lian-Yue Yang; Xiang Ding; Wei Wang

    2004-01-01

    AIM: To investigate the expression of cysteine-rich61 (Cyr61),connective tissue growth factor (CTGF) and nephroblastoma overexpressed gene (Nov) in hepatocellular carcinoma (HCC),and to evaluate the relationship between Cyr61, CTGF and Nov genes expression with invasion and metastasis of HCC.METHODS: Thirty-one HCC specimens were divided into small hepatocellular carcinoma (SHCC), nodular hepatocellular carcinoma (NHCC), solitary large hepatocellular carcinoma (SLHCC) according to their diameter and number of nodes. Reverse transcription polymerse chain reaction (RT-PCR) was used to detect the mRNA expression levels of Cyr61, CTGF and Nov genes in 31 resected specimens of hepatocellular carcinoma and para-cancerous normal liver tissues semi-quantitatively and the relation between their expression levels and clinical pathological parameters were compared.RESULTS: The expressions of Cyr61 and CTGF mRNA in carcinoma tissues were significantly higher than those in para-cancerous normal liver tissues (P<0.01). The expressions of Cyr61 and CTGF mRNA in HCC with venous invasion were higher than those in HCC without venous invasion. CTGF expression in HCC Edmondson's grade Ⅲ-Ⅳ was significantly higher than that in HCC Edmondson's grade Ⅰ-Ⅱ (P = 0.022). There was no obvious correlation between Nov mRNA and clinical-pathological features.Compared to NHCC, SLHCC had better cell differentiation,easier capsule formation, less microscopic venous invasion,milder liver cirrhosis. The expressions of Cyr61 and CTGF mRNA in NHCC were significantly higher than those in SLHCC and SHCC.CONCLUSION: Cyr61 and CTGF genes may play an important role in hepatocellular carcinogenesis and correlate with recurrence and metastasis of hepatocellular carcinoma.SLHCC has better biological behaviors than NHCC.

  5. The prognostic significance of hypoxia inducing factor 1-α in oropharyngeal cancer in relation to human papillomavirus status.

    Science.gov (United States)

    Hong, Angela; Zhang, Mei; Veillard, Anne-Sophie; Jahanbani, Jahanfar; Lee, C Soon; Jones, Deanna; Harnett, Gerald; Clark, Jonathan; Elliott, Michael; Milross, Chris; Rose, Barbara

    2013-04-01

    This study examines the prognostic significance of hypoxia inducing factor-1α (HIF-1α) expression in relation to human papillomavirus (HPV) status in oropharyngeal squamous cell carcinoma (SCC). Clinical details on 233 oropharyngeal SCCs were extracted from institutional databases. Recurrence in any form or death from any cause was recorded for a median of 51 months after diagnosis. HIF-1α expression was evaluated by semiquantitative immunohistochemistry and HPV status was determined by HPV E6-targeted multiplex real-time PCR and p16 immunohistochemistry. Determinants of recurrence and mortality hazards were modeled using Cox regression with censoring at dates of last follow-up. The HIF-1α positivity rate was 58.8%. HIF-1α positivity was associated with higher T category (T3/T4 vs. T1/T2, 64.2% vs. 48.4%, p=0.001) and lower grade (Grade 1-2 vs. 3, 62% vs. 46.9%, p=0.001). There was no significant association between HIF-1α expression and HPV status. After adjustment for clinico-pathological variables, HPV status but not HIF-1α was a strong predictor of outcome. The combination of HPV and HIF-1α was not a prognostic variable but the worst outcomes were seen in those with HPV negative and HIF-1α positive cancers. There was no statistically significant evidence of an interaction between HPV and HIF-1α. The degree of hypoxia as measured by HIF-1α expression does not differ between HPV positive and HPV negative cancers. The role of hypoxia in HPV negative oropharyngeal cancer warrants further investigation. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

  6. Prognostic significance of changes of tumor epidermal growth factor receptor expression after neoadjuvant chemoradiation in patients with rectal adenocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Dvorak, J.; Sirak, I.; Petera, J. [Charles Univ. Medical School and Teaching Hospital, Hradec Kralove (Czech Republic). Dept. of Oncology and Radiotherapy; Sitorova, V.; Ryska, A.; Hatlova, J. [Charles Univ. Medical School and Teaching Hospital, Hradec Kralove (Czech Republic). Dept. of Pathology; Richter, I. [Regional Hospital Liberec, Liberec (Czech Republic); Charles Univ. Medical School, Hradec Kralove (Czech Republic); Ferko, A. [Charles Univ. Medical School and Teaching Hospital, Hradec Kralove (Czech Republic). Dept. of Surgery; Melichar, B. [Palacky Univ. Medical School and Teaching Hospital, Olomouc (Czech Republic). Dept. of Oncology

    2012-09-15

    Purpose: The aim of the present study was to examine the effect of neoadjuvant chemoradiation on tumor epidermal growth factor receptor (EGFR) expression in patients with locally advanced rectal adenocarcinoma. Patients and methods: A total of 53 patients with rectal adenocarcinoma (clinical stages II and III) were studied. Neoadjuvant treatment consisted of 50.4 Gy/28 fractions external radiation with concomitant continuous 5-fluorouracil. Surgical resection was performed 4-6 weeks after the chemoradiation. EGFR expression in the pretreatment biopsies and in the resected specimens was assessed with immunohistochemistry. Results: Patients with an increase of EGFR expression during chemoradiation had significantly shorter disease-free survival (DFS; p = 0.003) and overall survival (OS; p = 0.005) compared to patients with either no change or decrease in EGFR expression. The 5-year DFS in patients with increased EGFR expression was only 29% compared to 61% in patients without an increase of EGFR expression. Similarly, the 5-year OS of the patients with increased EGFR expression was 29% compared to 66% in patients without an increase of EGFR expression. All recurrences in patients who had an increase of EGFR expression occurred within the first 2 years after the treatment. The increase in EGFR expression was the only significant predictor of DFS (p = 0.007) and OS (p = 0.04) using multivariate Cox regression analysis. Conclusion: An increase of EGFR expression during chemoradiation may be associated with significantly shorter DFS and OS. The increase of EGFR could identify a population of patients in whom the effect of the treatment with anti-EGFR therapy should be studied. (orig.)

  7. Expression and clinical significance of dendritic cell and transforming growth factor-beta 1 in cervical cancer

    Institute of Scientific and Technical Information of China (English)

    Zhao; Shan; Rong; Fengnian

    2006-01-01

    Objective:To explore the density and mature status of Dendritic cell(DC) in cervical cancer and correlation with the expression of transforming growth factor-beta 1(TGF-β1).Methods:Streptavidin-peroxidase(SP) immunohistochemistry methods were used to detect S-100 DC and the expression of TGF-β1 in 20 normal cervical tissues and 53 cervical cancer tissues without any sort of chemotherapy or radiation therapy prior to resection.Medical records were reviewed,clinicopathological variables were retrieved and used for analysis.Results:Two types of DC were observed under the microscope.The expression of DC in cervical cancer was significantly higher than that in normal tissues(23.34 cells/mm2 vs 29.91 cells/mm2,P<0.05),and significantly higher in early stage than that in advanced stage(P<0.05).The expression of TGF-β1 was significantly higher in cervical cancer than that in normal tissues (P<0.025).However,there was no correaction between TGF-β1 and lymph nodes metastasis.The index of DC in cervical cancer was negatively correlated to the expression of TGF-β1 in tumor cells (r=-0.8875,P=0.0001).Conclusion:Maturation of DC in cervical cancer is inhibited.The decreased number of DC and the higher expression of TGF-β1 are due to the failure of the immunity,these may play an important role in the development of the cervical cancer.

  8. Significant Correlation between Retinal Venous Tortuosity and Aqueous Vascular Endothelial Growth Factor Concentration in Eyes with Central Retinal Vein Occlusion.

    Science.gov (United States)

    Yasuda, Shunsuke; Kachi, Shu; Kondo, Mineo; Ueno, Shinji; Kaneko, Hiroki; Terasaki, Hiroko

    2015-01-01

    To determine whether the degree of venous tortuosity is significantly correlated with the aqueous vascular endothelial growth factor (VEGF) concentration in eyes with a central retinal vein occlusion (CRVO). We reviewed the medical records of 32 eyes of 32 patients who had macular edema due to a CRVO. All of the patients were examined at the Nagoya University Hospital and were scheduled to receive an intravitreal injection of bevacizumab (IVB) or ranibizumab (IVR) within 12 weeks of the onset of the CRVO to treat the macular edema. Aqueous humor was collected just before the IVB or IVR, and the VEGF concentration was determined by enzyme-linked immunosorbent assay (ELISA). The venous tortuosity index was calculated by dividing the length of the retinal veins by the chord length of the same segment. The correlation between the mean tortuosity index of the inferotemporal and supratemporal branches of the retinal vein and the aqueous VEGF concentration was determined. The mean aqueous VEGF concentration was 384 ± 312 pg/ml with a range of 90 to 1077 pg/ml. The degree of venous tortuosity was significantly correlated with the VEGF concentration in the aqueous. (r = 0.49, P = 0.004), with the foveal thickness (r = 0.40, P = 0.02), and with the best-corrected visual acuity (r = 0.38, P = 0.03). The significant correlation between the aqueous VEGF concentration and the venous tortuosity indicates that the degree of retinal venous tortuosity can be used to identify eyes that are at a high risk of developing neovascularization.

  9. A case report of motor neuron disease in a patient showing significant level of DDTs, HCHs and organophosphate metabolites in hair as well as levels of hexane and toluene in blood

    Energy Technology Data Exchange (ETDEWEB)

    Kanavouras, Konstantinos [Department of Neurology, Medical School, University of Crete, Heraklion, Crete (Greece); Tzatzarakis, Manolis N. [Center of Toxicology Science and Research, University of Crete, Heraklion, Crete (Greece); Mastorodemos, Vasileios; Plaitakis, Andreas [Department of Neurology, Medical School, University of Crete, Heraklion, Crete (Greece); Tsatsakis, Aristidis M., E-mail: aris@med.uoc.gr [Center of Toxicology Science and Research, University of Crete, Heraklion, Crete (Greece)

    2011-11-15

    Motor neuron disease is a devastating neurodegenerative condition, with the majority of sporadic, non-familial cases being of unknown etiology. Several epidemiological studies have suggested that occupational exposure to chemicals may be associated with disease pathogenesis. We report the case of a patient developing progressive motor neuron disease, who was chronically exposed to pesticides and organic solvents. The patient presented with leg spasticity and developed gradually clinical signs suggestive of amyotrophic lateral sclerosis, which was supported by the neurophysiologic and radiological findings. Our report is an evidence based case of combined exposure to organochlorine (DDTs), organophosphate pesticides (OPs) and organic solvents as confirmed by laboratory analysis in samples of blood and hair confirming systematic exposure. The concentration of non-specific dialkylphosphates metabolites (DAPs) of OPs in hair (dimethyphopshate (DMP) 1289.4 pg/mg and diethylphosphate (DEP) 709.4 pg/mg) and of DDTs (opDDE 484.0 pg/mg, ppDDE 526.6 pg/mg, opDDD 448.4 pg/mg, ppDDD + opDDT 259.9 pg/mg and ppDDT 573.7 pg/mg) were considerably significant. Toluene and n-hexane were also detected in blood on admission at hospital and quantified (1.23 and 0.87 {mu}g/l, respectively), while 3 months after hospitalization blood testing was found negative for toluene and n-hexane and hair analysis was provided decrease levels of HCHs, DDTs and DAPs. -- Highlights: Black-Right-Pointing-Pointer Exposure to pesticides and organic solvents might be a risk factor for sporadic MND. Black-Right-Pointing-Pointer We report a patient who developed progressive upper and lower motor neuron disease. Black-Right-Pointing-Pointer The patient had a history of occupational exposure to pesticides and solvents. Black-Right-Pointing-Pointer High DDTs' levels and increased levels of DMP and DEP were measured in his hair. Black-Right-Pointing-Pointer The patients' exposure to chemicals might

  10. The significance of biological, environmental, and social risk factors for prostate cancer in a cohort study in Brazil

    Directory of Open Access Journals (Sweden)

    Frederico R. Romero

    2012-12-01

    Full Text Available Purpose To evaluate the significance of several risk factors for prostate cancer in a cohort of Brazilian men. Subjects and methods: Men ≥ 40 years-old participating in a prostate cancer screening program between December 2006 and April 2011 in the city of Curitiba, Brazil, were evaluated to determine the prevalence, relative risk (RR and 95% CI of prostate cancer according to age, race, ethnicity, family history of prostate cancer, educational level, and history of vasectomy, increased blood pressure, diabetes mellitus, and urethritis. Results In 2121 men included in this study, prostate cancer prevalence was 0.6% for men between 40-49 years versus 2.0% (adjusted RR = 2.58, 7.7% (adjusted RR = 5.76, and 8.4% (adjusted RR = 4.88 for men 50-59 years, 60-69 years, and ≥ 70 years, respectively (p 0.05; 6.1% in African descendants, in comparison to 3.0% in non-African descendants (adjusted RR = 3.17, p 0.05; and 4.8% in participants with incomplete elementary school level or lower, compared to 2.2% in men with complete elementary school level or higher education (adjusted RR = 1.85, p > 0.05. Men with/without history of vasectomy, increased blood pressure, diabetes, and urethritis had a prostate cancer prevalence of 0.8%/3.0% (adjusted RR = 0.23, p > 0.05, 3.8%/2.2% (adjusted RR = 1.16, p > 0.05, 3.7%/2.6% (adjusted RR = 1.39, p > 0.05, and 2.6%/2.6% (adjusted RR = 0.99, p > 0.05, respectively. Conclusions Risk factors associated with an increased prevalence of prostate cancer in this cohort included increasing age and African ethnicity.

  11. Factors significantly related to science achievement of Malaysian middle school students: An analysis of TIMSS 1999 data

    Science.gov (United States)

    Mokshein, Siti Eshah

    The importance of science and technology in the global economy has led to growing emphasis on math and science achievement all over the world. In this study, I seek to identify variables at the student-level and school-level that account for the variation in science achievement of the eighth graders in Malaysia. Using the Third International Math and Science Study (TIMSS) 1999 for Malaysia, a series of HLM analysis was performed. Results indicate that (1) variation in overall science achievement is greater between schools than within schools; (2) both the selected student-level and school-level factors are Important in explaining the variation in the eight graders' achievement In science; (3) the selected student-level variables explain about 13% of the variation in students' achievement within schools, but as an aggregate, they account for a much larger proportion of the between-school variance; (4) the selected school-level variables account for about 55% of the variation between schools; (5) within schools, the effects of self-concept In science, awareness of the social implications of science, gender, and home educational resources are significantly related to achievement; (6) the effects of self-concept in science and awareness of social implications of science are significant even after controlling for the effects of SES; (7) between schools, the effects of the mean of home educational resources, mean of parents' education, mean of awareness of the social implications of science, and emphasis on conducting experiments are significantly related to achievement; (8) the effects of SES variables explain about 50% of the variation in the school means achievement; and (9) the effects of emphasis on conducting experiments on achievement are significant even after controlling for the effects of SES. Since it is hard to change the society, it is recommended that efforts to Improve science achievement be focused more at the school-level, concentrating on variables that

  12. Expression of transforming growth factor-α and hepatitis B surface antigen in human hepatocellular carcinoma tissues and its significance

    Institute of Scientific and Technical Information of China (English)

    Jing Zhang; Wen-Liang Wang; Qing Li; Qing Qiao

    2004-01-01

    AIM: To evaluate the expression of transforming growth factor-alpha (TGF-α) and hepatitis B surface antigen (HBsAg) in human hepatocellular carcinoma (HCC) tissues and its significance. METHODS: Seventy specimens of HCC tissues were detected by immunohistochemical method. Five specimens of normal human liver tissues were used as control. RESULTS: The TGF-α positive expression rates in HCC and its surrounding tissues were 74.3%(52/70) and 88. t%(52/59), respectively. TGF-α positive granules were mainly in the cytoplasm and fewer existed on the karyotheca. The TGF-α positive expressing rate in well differentiated HCC was significantly higher than that in moderately and poorly differentiated HCC (P<0.05). The TGF-α positive expression also was observed in intrahepatic bile ducts (part of those were hyperplastic ducts). The HBsAg positive expression rates in HCC and its surrounding tissues were 21.4%(15/70) and 79.7%(47/59), respectively. HBsAg positive granules were in the cytoplasm, inclusion and on the karyotheca. There was a prominent positive correlation between TGF-α and HBsAg expression in HCC surrounding tissues (P<0.05, γ=0.34). TGF-α was usually existed with HBsAg in regenerated and/or dysplastic liver cells. In the five normal liver tissues, TGF-α and HBsAg were not detectable in hepatocytes and bile ducts.CONCLUSION: Hepatitis B virus infection is closely related with hepatocarcinogenesis. The overexpression of TGF-α in the liver seems to be associated with the regeneration of hepatocytes injured by HBsAg. The continued expression of TGF-α might lead to dysplasia of liver cells and development of HCC. Furthermore, TGF-α might play a role in morphogenesis and regeneration of intrahepatic bile ducts.

  13. Detection and clinical significance of rheumatoid factor, anti -CCP antibody, immunoglobulin and cytokines in serum of patients with rheumatoid arthritis

    Institute of Scientific and Technical Information of China (English)

    Hui-Ya Zhu; Qian Feng

    2016-01-01

    Objective:To detect the content of rheumatoid factor, ring citrulline peptide antibody (CCP), immunoglobulin (Ig) and cytokines in serum of patients with rheumatoid arthritis (RA) and explore the clinical application value of serum indexes for RA diagnosis, treatment and prognostic.Methods: A total of 98 patients with RA were selected, of which 52 cases in the activity and 46 patients in remission, 65 healthy people as controls. Contents of serum RF and Ig (IgM, IgG, IgA) in every group were detected by methods of immune scattering turbidimetry respectively; the content of serum anti-CCP antibody and cytokines IL-1β, TNF-α, IFN-γ and IL-4 were measured by methods of enzyme-linked immunosorbent respectively. Change of detection index levels between groups were compared and the correlation between cytokines and RF, anti CCP, Ig and DAS28 score in patients with RA were analyzed.Results:The contents of serum RF, CCP antibody, Ig (IgM and IgG, IgA) of RA group (activity and remission) were respectively significantly higher than that of the control group (P0.05). The levels of cytokine IL-1β, TNF-α, IFN-γ were positively correlated with RF, CCP antibody, Ig and DAS28 scores in RA activity while which were only positively correlated with DAS28 scores of RA in remission, and the IL-4 level had no significant correlation with other indicators. Conclusions:The detection of levels of RF, CCP antibody, Ig and cytokine for the diagnosis, treatment and prognosis of RA has important reference value.

  14. Significance of plasma von Willebrand factor level and von Willebrand factor-cleaving protease activity in patients with chronic renal diseases

    Institute of Scientific and Technical Information of China (English)

    LU Guo-yuan; SHEN Lei; WANG Zhao-yue; GUO Xiao-fang; BAI Xia; SU Jian; RUAN Chang-geng

    2008-01-01

    Background yon Willebrand factor(vWF)mediates the initial capture of platelets to vascular subendothelium and is essential for platelet aggregation under high fluid shear stress as in arteriaI stenosis.On release frOm endothelial cells,vWF is rapidly cleaved by ADAMTSl 3/vWF-cleaving protease (vWF-CP).We investigated the clinical significance of changes in plasma vWF and vWF-CP activities in chronic renal disease.Methods Plasma vWF and vWF-CP activities were measured using enzyme-linked immunosorbent assay(ELISA)and residual collagen binding assay respectively in patients with lupus nephritis(n=31),primary nephritic syndrome(n=25),diabetic nephropathy(n=45),chronic glomerulonephritis(n=38)and 40 normal controls.The reIation of their levels with pathological and renal status was analyzed.Results In all diseased patients the levels of vWF were significantly higher and vWF-CP activity significantly lower than the controls(both P<0.01).vWF in the four subgroups did not correlate with the stage of disease but correlated negatively with vWF-CP activity.vWF-CP activity was not changed two weeks after renal transplantation.Renal biopsy demonstrated that the vWF level in stage Ⅳ was higher than in stages Ⅱ and Ⅲ while vWF-CP activity was lower in patients with lupus nephritis.After eight-week treatment,the vWF level significantly decreased and the vWF-CP activity significantly increased in systemic lupus erythema,disease activity index<9,but not with index≥9.Even though the vWF-CP activity was significantly lower in membranous nephropathy than in minimal change disease,mesangial proliferative glomerulonephritis or IgA glomerulonephritis,the vWF level was not significantly different.Conclusions The alterations of plasma vWF and vWF-CP activities were associated with different renal pathologies.Injury to endothelial cells and autoantibodies against vWF-CP activity may result in higher vWF Ievel and Iower vWF-CP activity in chronic renaI disease and thus a

  15. The significance of life-events as contributing factors in childhood recurrent abdominal pain in an urban community in Malaysia.

    Science.gov (United States)

    Boey, C C; Goh, K L

    2001-10-01

    This study aimed to look at the link between childhood recurrent abdominal pain (RAP) and the presence of recent life-events in an urban community in Malaysia. School children aged from 9 to 15 years in the city of Petaling Jaya were randomly selected to fill in a questionnaire and to be interviewed. The prevalence of RAP among 1488 school children studied was 9.6% (95% confidence interval (CI), 8.18-11.25). Higher prevalences of RAP were found in children who had experienced the following life-events in the previous year: loss of a family member through death (Pbullying at school (P=.001). Following logistic regression analysis, five life-events remain significant: hospitalisation of a family member (P=.038), the child's own hospitalisation (P=.034), change in occupation of an immediate family member (P=.049), examination failure (P=.001) and bullying at school (P=.028). This study strongly suggests that recent stressful life-events are important risk-factors for RAP.

  16. Climatic fluctuations as a significant contributing factor for volcanic collapses. Evidence from Mexico during the Late Pleistocene

    Science.gov (United States)

    Capra, L.; Bernal-Uruchurtu, J. P.; Carrasco, G.

    2013-05-01

    Climate oscillations have significantly contributed to the planet's evolution, including volcanic activity. Major glaciations have been considered not only as a triggering mechanism for large magmatic eruptions but also inducing volcano instability. Generally, volcano instability can be inferred from detailed volcanological and structural studies of a volcano and its associated depositional sequence, but the triggering mechanism has been always difficult to infer. In this paper, we present evidence of how climatic variations during the Late Pleistocene could have forced sector collapses of the main Mexican stratovolcanoes and enhanced the mobility of associated massive flows inducing the transformation of debris avalanche into debris flows. In particular, the climatic record based on atmospheric moisture content from robustly dated lake record from Guatemala and a U/Th dated speleothem from New Mexico are used here as indicators of summer and winter precipitation. Depositional sequences associated with Late Pleistocene sector collapses of Volcan de Colima, Nevado de Toluca, Citlaltepetl (Pico de Orizaba) and Cofre de Perote volcanoes are here analyzed. Comparing the timing of the event with the climatic record, a combination of summer and/or winter pluvial conditions could have forced and triggered the failure of already unstable volcanoes, even during glacier advances (as for the Citlaltepetl event). Independently of the main cause of the volcano instability (magmatic or tectonic) it is important to highlight that the climatic factor played an important role in enhancing the volcano instability and promoted the lateral transformation of debris avalanches, which under dry conditions would have affected more limited areas.

  17. Prevalence of JAK2V617F mutation in deep venous thrombosis patients and its clinical significance as a thrombophilic risk factor: Indian perspective.

    Science.gov (United States)

    Singh, Neha; Sharma, Amit; Sazawal, Sudha; Ahuja, Ankur; Upadhyay, Ashish; Mahapatra, Manoranjan; Saxena, Renu

    2015-09-01

    Venous thromboembolism is known to be a complex interaction of genetic and acquired factors leading to thrombosis. JAK2V617F mutation is believed to contribute to a thrombophilic phenotype, possibly through enhanced leukocyte-platelet interactions in myeloproliferative neoplasms (MPNs). Several studies have focused on the importance of screening for JAK2V617F mutation in patients with splanchnic venous thrombosis (VT) for the detection of nonovert MPNs. The role of JAK2V617F mutation in VT outside the splanchnic region is still widely unsettled. The primary aim of this study was to find out the prevalence of JAK2V617F mutation in patients with deep venous thrombosis (DVT), its clinical significance as a prothrombotic risk factor, and its possible interactions with other genetic thrombophilic risk factors. A total of 148 patients with idiopathic, symptomatic DVT were evaluated. Median age of presentation was 32 years (range 15-71 years) with a sex ratio of 1.3:1. Overall, the most common genetic prothrombotic factor was factor V Leiden mutation, found in 10.8% (16 of 148) of patients who also showed strong association with increased risk of thrombosis (odds ratio 5.94, confidence interval 1.33-26.4, P = .019). Deficiencies in protein C, protein S, and antithrombin were seen in 8 (5.4%), 10 (6.7%), and 8 (5.4%) patients, respectively. It was observed that the frequency of JAK2V617F mutation was lower in Indian patients, and it also showed weaker association with risk of thrombosis, at least in cases of venous thrombosis outside the splanchnic region.

  18. The clinical significance and risk factors of anti-platelet factor 4/heparin antibody on maintenance hemodialysis patients: a two-year prospective follow-up.

    Directory of Open Access Journals (Sweden)

    Delong Zhao

    Full Text Available BACKGROUND: Heparin-induced thrombocytopenia is an immune response mediated by anti-PF4/heparin antibody, which is clinically characterized by thrombocytopenia and thromboembolic events. In this study, a prospective and multi-center clinical investigation 1 determined the positive rate of anti-PF4/heparin antibody in maintenance hemodialysis patients in China, 2 identified the related risk factors, and 3 further explored the effect of the anti-PF4/heparin antibody on bleeding, thromboembolic events, and risk of death in the patients. METHODS: The serum anti-PF4/heparin antibody was measured in 661 patients from nine hemodialysis centers, detected by IgG-specific ELISA and followed by confirmation with excess heparin. Risk factors of these patients were analyzed. Based on a two-year follow-up, the association between the anti-PF4/heparin antibody and bleeding, thromboembolic events, and risk of death in the patients was investigated. RESULTS: 1 The positivity rate of the anti-PF4/heparin antibody in maintenance hemodialysis patients was 5.6%. With diabetes as an independent risk factor, the positivity rate of the anti-PF4/heparin antibody decreased in the patients undergoing weekly dialyses ≥3 times. 2 The positivity rate of the anti-PF4/heparin antibody was not related to the occurrence of clinical thromboembolic events and was not a risk factor for death within two years in maintenance hemodialysis patients. 3 Negativity for the anti-PF4/heparin antibody combined with a reduction of the platelet count or combined with the administration of antiplatelet drugs yielded a significant increase in bleeding events. However, the composite determination of the anti-PF4/heparin antibody and thrombocytopenia, as well as the administration of antiplatelet drugs, was not predictive for the risk of thromboembolic events in the maintenance hemodialysis patients. CONCLUSIONS: A single detection of the anti-PF4/heparin antibody did not predict the occurrence

  19. [A Case of Crohn's Disease Showing Favorable Response to Induction and Maintenance Therapy with Methotrexate after Failure of Anti-tumor Necrosis Factor Therapy].

    Science.gov (United States)

    Choi, Jung Ran; Yun, Gak Won; Park, Yoo Mi; Kim, Jie Hyun; Youn, Young Hoon; Park, Hyo Jin; Park, Jae Jun

    2015-10-01

    Thanks to the introduction of immumomodulators and biologics, therapeutic approaches in Crohn's disease have changed significantly during the past decade. Although new biologic therapy has dramatically improved the treatment of Crohn's disease, a substantial number of patients are refractory to these therapies or lose their initial response. Methotrexate (MTX) is a structural analogue of folic acid that can competitively inhibit the binding of dihydrofolic acid to the enzyme dihydrofolate reductase and has been widely used as immunomodulator in rheumatology area for patients with rheumatoid arthritis and psoriasis. Although MTX has also been shown to be an effective agent for remission induction and maintenance of remission in Crohn's disease, the use of MTX in Crohn's disease has not yet been reported in Korea. Herein, we report a case of Crohn's disease patient who was successfully treated with MTX after treatment failure with thiopurine and anti-tumor necrosis factor.

  20. Significance of changes in transforming growth factor-β mRNA levels in autogenous vein grafts

    Institute of Scientific and Technical Information of China (English)

    尤文俊; 萧明第; 袁忠祥

    2004-01-01

    Background This study was designed to investigate changes in mRNA levels of transforming growth factor-β(TGF-β), collagen Ⅰ, and collagen Ⅲ in autogenous vein grafts. Methods Twenty-four New Zealand rabbits were randomly divided into 4 groups with 6 rabbits each. The external jugular veins of the New Zealand rabbits were harvested and grafted into the ipsilateral carotid artery. All rabbits were fed with a standard diet. After the operation, the rabbits were sacrificed at 1, 2, 3, or 4 weeks. TGF-β, collagen Ⅰ, and collagen Ⅲ mRNA levels in the venous grafts were measured by semiquantitative methods at every time point. The contralateral external jugular veins were also harvested and analyzed as controls. Glyceraldehyde-3-phosphate dehydrogenase was used as an internal standard to normalize all samples for potential variations in mRNA content. In order to observe the expression of TGF-β protein, immunohistochemical SABC methods were used. Results One week postoperation, the mRNA level of TGF-β was upregulated to 1.73±0.19 in the vein graft and 1.21±0.16 in the control vein (P<0.01). High mRNA levels were maintained until week 4 postoperation. The mRNA levels of collagen Ⅰ and collagen Ⅲ were also significantly increased to 2.18±0.21 versus 1.12±0.24 and 1.08±0.13 versus 0.83±0.12, respectively (P<0.05). Immunohistochemical staining revealed a higher density of TGF-β expression in the vein grafts.Conclusions An uninterrupted increase in mRNA levels of TGF-β, collagen Ⅰ, and collagen Ⅲ is observed in autogenous vein grafts. This increase may be the major cause of intimal hyperplasia, sclerosis, and even graft failure.

  1. A Variant Form of the Human Deleted in Malignant Brain Tumor 1 (DMBT1) Gene Shows Increased Expression in Inflammatory Bowel Diseases and Interacts with Dimeric Trefoil Factor 3 (TFF3)

    DEFF Research Database (Denmark)

    Madsen, Jens; Sorensen, Grith Lykke; Nielsen, Ole Stig;

    2013-01-01

    The protein deleted in malignant brain tumors (DMBT1) and the trefoil factor (TFF) proteins have all been proposed to have roles in epithelial cell growth and cell differentiation and shown to be up regulated in inflammatory bowel diseases. A panel of monoclonal antibodies was raised against human......-1 showed no reaction. Hyb213-6 showed strong cytoplasmic staining in epithelial cells of both the small and large intestine whereas no staining was seen with Hyb213-1. The number of DMBT1(gp340) positive epithelial cells, stained with Hyb213-6, was significantly up regulated in inflammatory colon tissue...

  2. Post-migration living difficulties as a significant risk factor for PTSD in immigrants: a primary care study

    Directory of Open Access Journals (Sweden)

    Massimilano Aragona

    2012-09-01

    Full Text Available

    Background: recent research shows that severe/very severe post-migration living difficulties (PMLD have a negative impact on the mental health and social integration of refugees and asylum seekers. This study focuses on the role of PMLD in primary care “ordinary” immigrants.

    Methods: 443 primary care immigrants were asked to complete a self-administered questionnaire measuring the number and severity of pre-migratory potentially traumatic events (PTE, PMLD, and the current prevalence of a post-traumatic stress disorder (PTSD. The frequency of PMLD was assessed in the whole sample and compared in patients with and without PTSD. The effect of the number of PMLD on the risk of having a PTSD was studied by means of a regression analysis, adjusted by the number of PTE.

    Results: 391 patients completed the questionnaire and were enrolled into the study. The prevalence of PTSD was 10.2%. In the whole sample the most frequent PMLD were “no permission to work” (38.6% and “poverty” (34.5%. All PMLD (except “communication difficulties” were more frequent in patients with a PTSD. The number of PMLD significantly increased the likelihood to have a PTSD independently from PTE. Conclusions: severe/very severe post-migration living difficulties (PMLD increase significantly the risk of PTSD in primary care “ordinary” migrants. Our hypothesis is that they have a retraumatizing effect on individuals who are already vulnerable and with a low capacity to handle resettlement stress due to their previous traumatic history. The implications in clinical practice and for immigration policies are discussed.

  3. Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease.

    Science.gov (United States)

    Liu, Xin; Yang, Lihua; Zhou, Xianyao; Zhou, Miaoping; Lu, Yan; Ma, Lingjian; Ma, Hongxiang; Zhang, Zengyan

    2013-05-01

    The disease take-all, caused by the fungus Gaeumannomyces graminis, is one of the most destructive root diseases of wheat worldwide. Breeding resistant cultivars is an effective way to protect wheat from take-all. However, little progress has been made in improving the disease resistance level in commercial wheat cultivars. MYB transcription factors play important roles in plant responses to environmental stresses. In this study, an R2R3-MYB gene in Thinopyrum intermedium, TiMYB2R-1, was cloned and characterized. The gene sequence includes two exons and an intron. The expression of TiMYB2R-1 was significantly induced following G. graminis infection. An in vitro DNA binding assay proved that TiMYB2R-1 protein could bind to the MYB-binding site cis-element ACI. Subcellular localization assays revealed that TiMYB2R-1 was localized in the nucleus. TiMYB2R-1 transgenic wheat plants were generated, characterized molecularly, and evaluated for take-all resistance. PCR and Southern blot analyses confirmed that TiMYB2R-1 was integrated into the genomes of three independent transgenic wheat lines by distinct patterns and the transgene was heritable. Reverse transcription-PCR and western blot analyses revealed that TiMYB2R-1 was highly expressed in the transgenic wheat lines. Based on disease response assessments for three successive generations, the significantly enhanced resistance to take-all was observed in the three TiMYB2R-1-overexpressing transgenic wheat lines. Furthermore, the transcript levels of at least six wheat defence-related genes were significantly elevated in the TiMYB2R-1 transgenic wheat lines. These results suggest that engineering and overexpression of TiMYB2R-1 may be used for improving take-all resistance of wheat and other cereal crops.

  4. Hypoxia related growth factors and p53 in preoperative sera from patients with colorectal cancer--evaluation of the prognostic significance of these agents.

    Science.gov (United States)

    Sulkowski, Stanislaw; Wincewicz, Andrzej; Zalewski, Bogdan; Famulski, Waldemar; Lotowska, Joanna Maria; Koda, Mariusz; Sobaniec-Lotowska, Maria Elzbieta; Mysliwiec, Michal; Baltaziak, Marek; Pawlak, Krystyna; Sulkowska, Mariola

    2009-01-01

    Insulin-like growth factor-I (IGF-I) and vascular endothelial growth factor (VEGF) belong to a group of hypoxia related proteins. IGF-I induces expression of VEGF and decomposes wild type p53 in cancer cell lines. The goal of our study was to evaluate serum IGF-I, VEGF and p53 with respect to overall and disease free survival of patients with colorectal cancer (CRC) patients compared with healthy volunteers. Preoperative blood samples from 125 patients with CRC and 16 healthy volunteers were examined using ELISA for serum IGF-I, p53 and VEGF concentrations. Concentrations of p53 and VEGF were significantly higher in CRC patients than in controls (p<0.0006 and p<0.0001, respectively). IGF-I was not statistically different between both groups. Serum IGF-I showed negative correlation with p53 in CRC patients (p<0.04, r=-0.193). IGF-I and VEGF showed negative correlation in poorly differentiated cancers (G3) (p<0.03, r=-0.339). Patients with VEGF concentrations that were above average for the cancer population survived for a shorter period of time (p=0.065 in evaluation of overall survival and 0.071 in estimation of disease-free survival during a 3-year follow-up) compared with patients with serum VEGF lower than the highest values seen in controls. Comparisons between serum IGF-I and p53 appear to confirm the metabolism of p53 by IGF-I. Serum VEGF showed prognostic significance in our study. Serum concentrations of IGF-I and VEGF did not show positive correlation, as expected due to IGF-I induction of VEGF in malignant colon cell lines.

  5. Detection and significance of serum interleukin-6, tumor necrosis factor-α and interleukin-1β in first-episode schizophrenia patients

    Institute of Scientific and Technical Information of China (English)

    Tao Yan; Chao Cheng; Hui-Xia Wang; Yuan-Yuan Li; Shuai-Bin Zhang

    2015-01-01

    Objective:To detect changes of serum interleukin-6, tumor necrosis factor-α and interleukin-1β in first-episode and different subtypes, and to analyze the correlation between pathological and immune mechanism.Methods:90 first-episode schizophrenia patients admitted to our hospital were enrolled as observation group, and were subdivided into four groups including negative and positive , family type and distributing types. During the same period, 35 physical healthy volunteers were enrolled as control group. Serum interleukin-6, tumor necrosis factor-αand interleukin-1β in both observation group and control group were detected with enzyme-linked immunosorbent (ELISA), correlation between these factors and mental symptoms were analyzed with pearson correlation analysis.Results:The levels of serum IL-6, TNF-αα, IL-1β of the observation group before and after the treatment were significantly higher than those of control group, the level of IL-1β in observation group after treatment was significantly lower than that before the treatment (P<0.05). Before treatment, the level of IL-6 in family type was significantly higher than that in distributing type, the level of TNF-αα in negative type was significantly higher than that in positive type. After treatment the level of TNF-αα was significantly reduced in family type and negative type, the IL-1β was significantly reduced in four subtypes. Pearson correlation analysis showed that TNF-αα lever was positively correlated with symptoms of negative subtype. IL-1β level and PANSS scores, symptoms of both positive and negative types were positively correlated.Conclusion:The levels of IL-6, TNF-αα, IL-1β levels are significantly increases in patients with first-episode schizophrenia, immune activation may be activated and IL-6 level is closely related to the family type of the disease which is genetic influenced, TNF-αα is associated with symptoms of negative type. IL-1β level may be a certain extent

  6. The Arabidopsis homologs of CCR4-associated factor 1 show mRNA deadenylation activity and play a role in plant defence responses

    Institute of Scientific and Technical Information of China (English)

    Wenxing Liang; Changbao Li; Fang Liu; Hongling Jiang; Shuyu Li; Jiaqiang Sun; Xiaoyan Wu; Chuanyou Li

    2009-01-01

    Messenger RNA (mRNA) turnover in eukaryotic cells begins with shortening of the poly (A) tail at the 3' end, a process called deadenylation. In yeast, the deadenylation reaction is predominantly mediated by CCR4 and CCR4-associated factor 1 (CAF1), two components of the well-characterised protein complex named CCR4-NOT. We re-port here that AtCAFla and AtCAFlb, putative Arabidopsis homologs of the yeast CAFI gene, partially complement the growth defect of the yeast cafl mutant in the presence of caffeine or at high temperatures. The expression of At-CAFla and AtCAFlb is induced by multiple stress-related hormones and stimuli. Both AtCAFIa and AtCAFIb show deadenylation activity in vitro and point mutations in the predicted active sites disrupt this activity. T-DNA inser-tion mutants disrupting the expression of AtCAFla and/or AtCAFlb are defective in deadenylation of stress-related mRNAs, indicating that the two AtCAFI proteins are involved in regulated mRNA deadenylation in vivo. Interest-ingly, the single and double mutants of AtCAFla and AtCAFlb show reduced expression of pathogenesis-related (PR) genes PR1 and PR2 and are more susceptible to Pseudomonas syringae pv tomato DC3000 (Pst DC3000) infection, whereas transgenic plants over-expressing AtCAFla show elevated expression of PR1 and PR2 and increased resis-tance to the same pathogen. Our results suggest roles of the AtCAF1 proteins in regulated mRNA deadenylation and defence responses to pathogen infections.

  7. Reversal of apixaban induced alterations in hemostasis by different coagulation factor concentrates: significance of studies in vitro with circulating human blood.

    Directory of Open Access Journals (Sweden)

    Gines Escolar

    Full Text Available Apixaban is a new oral anticoagulant with a specific inhibitory action on FXa. No information is available on the reversal of the antihemostatic action of apixaban in experimental or clinical settings. We have evaluated the effectiveness of different factor concentrates at reversing modifications of hemostatic mechanisms induced by moderately elevated concentrations of apixaban (200 ng/ml added in vitro to blood from healthy donors (n = 10. Effects on thrombin generation (TG and thromboelastometry (TEM parameters were assessed. Modifications in platelet adhesive, aggregating and procoagulant activities were evaluated in studies with blood circulating through damaged vascular surfaces, at a shear rate of 600 s(-1. The potential of prothrombin complex concentrates (PCCs; 50 IU/kg, activated prothrombin complex concentrates (aPCCs; 75 IU/kg, or activated recombinant factor VII (rFVIIa; 270 μg/kg, at reversing the antihemostatic actions of apixaban, were investigated. Apixaban interfered with TG kinetics. Delayed lag phase, prolonged time to peak and reduced peak values, were improved by the different concentrates, though modifications in TG patterns were diversely affected depending on the activating reagents. Apixaban significantly prolonged clotting times (CTs in TEM studies. Prolongations in CTs were corrected by the different concentrates with variable efficacies (rFVIIa≥aPCC>PCC. Apixaban significantly reduced fibrin and platelet interactions with damaged vascular surfaces in perfusion studies (p<0.05 and p<0.01, respectively. Impairments in fibrin formation were normalized by the different concentrates. Only rFVIIa significantly restored levels of platelet deposition. Alterations in hemostasis induced by apixaban were variably compensated by the different factor concentrates investigated. However, effects of these concentrates were not homogeneous in all the tests, with PCCs showing more efficacy in TG, and rFVIIa being more effective

  8. Versatile reporter systems show that transactivation by human T-cell leukemia virus type 1 Tax occurs independently of chromatin remodeling factor BRG1.

    Science.gov (United States)

    Zhang, Ling; Liu, Meihong; Merling, Randall; Giam, Chou-Zen

    2006-08-01

    Potent activation of human T-cell leukemia virus type 1 (HTLV-1) gene expression is mediated by the virus-encoded transactivator protein Tax and three imperfect 21-bp repeats in the viral long terminal repeats. Each 21-bp repeat contains a cAMP-responsive-element core flanked by 5' G-rich and 3' C-rich sequences. Tax alone does not bind DNA. Rather, it interacts with basic domain-leucine zipper transcription factors CREB and ATF-1 to form ternary complexes with the 21-bp repeats. In the context of the ternary complexes, Tax contacts the G/C-rich sequences and recruits transcriptional coactivators CREB-binding protein (CBP)/p300 to effect potent transcriptional activation. Using an easily transduced and chromosomally integrated reporter system derived from a self-inactivating lentivirus vector, we showed in a BRG1- and BRM1-deficient adrenal carcinoma cell line, SW-13, that Tax- and 21-bp repeat-mediated transactivation does not require BRG1 or BRM1 and is not enhanced by BRG1. With a similar reporter system, we further demonstrated that Tax- and tumor necrosis factor alpha-induced NF-kappaB activation occurs readily in SW-13 cells in the absence of BRG1 and BRM1. These results suggest that the assembly of stable multiprotein complexes containing Tax, CREB/ATF-1, and CBP/p300 on the 21-bp repeats is the principal mechanism employed by Tax to preclude nucleosome formation at the HTLV-1 enhancer/promoter. This most likely bypasses the need for BRG1-containing chromatin-remodeling complexes. Likewise, recruitment of CBP/p300 by NF-kappaB may be sufficient to disrupt histone-DNA interaction for the initiation of transcription.

  9. Follow-up of women with cervical cytological abnormalities showing atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion: a nationwide cohort study.

    Science.gov (United States)

    Sundström, Karin; Lu, Donghao; Elfström, K Miriam; Wang, Jiangrong; Andrae, Bengt; Dillner, Joakim; Sparén, Pär

    2017-01-01

    Atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion in abnormal cervical cytology among young women in cervical cancer screening is an increasing health burden, and comparative effectiveness studies of different management options for such diagnoses are needed. The objective of the study was to compare the incidence of invasive cervical cancer, following different management options pursued after an atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesion index smear. In this nationwide cohort study, we included all women aged 22-50 years and resident in Sweden 1989-2011 and with at least 1 cervical smear registered during the study period (n = 2,466,671). Follow-up of a first atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesion cytological diagnosis within 25 months was classified as repeat cytology, colposcopy/biopsy, or without further assessment. Incidence rate ratios and 95% confidence intervals of subsequent cervical cancer within 6.5 years following atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesion were estimated using Poisson regression by age group and management strategy. Women managed with repeat cytology within 6 months after atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion cytology had a similar risk of cervical cancer compared with colposcopy/biopsy (incidence rate ratio, 1.1, 95% confidence interval, 0.5-2.5, and incidence rate ratio, 2.0, 95% confidence interval, 0.6-6.5, respectively) among women aged 22-27 years. For women aged 28 years and older, women managed with repeat cytology had a higher risk for cervical cancer than women managed with colposcopy/biopsy. Our findings suggest that women with a first cytological diagnosis of atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesion up to

  10. A significant breakthrough in the incidence of childhood cancers and evaluation of its risk factors in Southern Iran

    Directory of Open Access Journals (Sweden)

    Asma Erjaee

    2017-01-01

    Full Text Available Background and Objective: This study investigates epidemiologic and practical information about the incidence and risk factors of childhood cancer in a population of Southern Iranian children. Materials and Methods: A total number of 300 cancer patients along with 600 age- and gender-matched healthy control were interviewed by a trained physician regarding their demographic characteristics, and major family-associated risk factors, childhood malignancies. Results: The average annual percentage change for cancers in the studied population is calculated as 45%. Our study indicated that possible risk factors which could contribute to the development of childhood cancer are maternal oral contraceptive pill use during pregnancy, exposure to radiation during pregnancy, parental smoking, residence near high voltage electricity lines, exposure to pesticides and fertilizers, patient allergy, contact with domestic animals and father's educational degree. Furthermore, new ecological risk factors such as air pollution due to nonstandard petroleum or toxic inhalant particles, nonhealthy food consumption, and satellite jamming are other predisposing factors. Conclusion: Our study reported a higher average annual percentage change of childhood cancers in our area, compared to the existing literature. In conclusion, detection and prevention of the consistent and possible new environmental risk factors such as nonstandard petroleum or satellite jamming from all around the country should be taking into consideration.

  11. A Significant Breakthrough in the Incidence of Childhood Cancers and Evaluation of its Risk Factors in Southern Iran.

    Science.gov (United States)

    Erjaee, Asma; Niknam, Maryam; Sadeghi, Ahmadreza; Dehghani, Maryam; Safaei, Zeinab; Teshnizi, Saeed Hosseini; Karimi, Mehran

    2017-01-01

    This study investigates epidemiologic and practical information about the incidence and risk factors of childhood cancer in a population of Southern Iranian children. A total number of 300 cancer patients along with 600 age- and gender-matched healthy control were interviewed by a trained physician regarding their demographic characteristics, and major family-associated risk factors, childhood malignancies. The average annual percentage change for cancers in the studied population is calculated as 45%. Our study indicated that possible risk factors which could contribute to the development of childhood cancer are maternal oral contraceptive pill use during pregnancy, exposure to radiation during pregnancy, parental smoking, residence near high voltage electricity lines, exposure to pesticides and fertilizers, patient allergy, contact with domestic animals and father's educational degree. Furthermore, new ecological risk factors such as air pollution due to nonstandard petroleum or toxic inhalant particles, nonhealthy food consumption, and satellite jamming are other predisposing factors. Our study reported a higher average annual percentage change of childhood cancers in our area, compared to the existing literature. In conclusion, detection and prevention of the consistent and possible new environmental risk factors such as nonstandard petroleum or satellite jamming from all around the country should be taking into consideration.

  12. Late-Life Environmental Enrichment Induces Acetylation Events and Nuclear Factor κB-Dependent Regulations in the Hippocampus of Aged Rats Showing Improved Plasticity and Learning.

    Science.gov (United States)

    Neidl, Romain; Schneider, Anne; Bousiges, Olivier; Majchrzak, Monique; Barbelivien, Alexandra; de Vasconcelos, Anne Pereira; Dorgans, Kevin; Doussau, Frédéric; Loeffler, Jean-Philippe; Cassel, Jean-Christophe; Boutillier, Anne-Laurence

    2016-04-13

    Aging weakens memory functions. Exposing healthy rodents or pathological rodent models to environmental enrichment (EE) housing improves their cognitive functions by changing neuronal levels of excitation, cellular signaling, and plasticity, notably in the hippocampus. At the molecular level, brain derived-neurotrophic factor (BDNF) represents an important player that supports EE-associated changes. EE facilitation of learning was also shown to correlate with chromatin acetylation in the hippocampus. It is not known, however, whether such mechanisms are still into play during aging. In this study, we exposed a cohort of aged rats (18-month-old) to either a 6 month period of EE or standard housing conditions and investigated chromatin acetylation-associated events [histone acetyltranferase activity, gene expression, and histone 3 (H3) acetylation] and epigenetic modulation of the Bdnf gene under rest conditions and during learning. We show that EE leads to upregulation of acetylation-dependent mechanisms in aged rats, whether at rest or following a learning challenge. We found an increased expression of Bdnf through Exon-I-dependent transcription, associated with an enrichment of acetylated H3 at several sites of Bdnf promoter I, more particularly on a proximal nuclear factor κB (NF-κB) site under learning conditions. We further evidenced p65/NF-κB binding to chromatin at promoters of genes important for plasticity and hippocampus-dependent learning (e.g., Bdnf, CamK2D). Altogether, our findings demonstrate that aged rats respond to a belated period of EE by increasing hippocampal plasticity, together with activating sustained acetylation-associated mechanisms recruiting NF-κB and promoting related gene transcription. These responses are likely to trigger beneficial effects associated with EE during aging. Aging weakens memory functions. Optimizing the neuronal circuitry required for normal brain function can be achieved by increasing sensory, motor, and

  13. [Factors influencing survival and recurrence and potential significance of postoperative radiotherapy and adjuvant chemotherapy for stage ⅢA-N2 non-small cell lung cancer].

    Science.gov (United States)

    Han, W; Song, Y Z; He, M; Li, J; Zhang, R; Qiao, X Y

    2016-11-23

    Objective: To investigate the survival, recurrence patterns and risk factors in patients with stage ⅢA-N2 NSCLC treated with curative surgery and adjuvant chemotherapy and to explore the significance of postoperative radiation therapy. Methods: The clinical data of 290 patients with pathologically diagnosed stage ⅢA-N2 NSCLC after curative resection and adjuvant chemotherapy from January 2010 to December 2014 at our department were retrospectively analyzed. The survival and recurrence patterns were observed, and the factors affecting locoregional recurrence were analyzed. Results: The median survival time was 31.5 months. The 1-, 3-and 5-year survival rates were 88.3%, 46.0% and 33.2%, respectively. The median locoregional control time was 38.5 months. The 1-, 3-and 5-year locoregional control rates were 78.6%, 55.2% and 41.0%, respectively. The median distant metastasis-free survival was 26.8 months. The 1-, 3-and 5-year distant metastasis-free survival rates were 76.4%, 45.5% and 39.5%, respectively. The median progression-free survival was 19.1 months. The 1-, 3-and 5-year progression-free survival rates were 64.1%, 32.5% and 23.8%, respectively. Univariate analysis showed that clinical N status, histological type, pathological T stage, operation mode, the number of positive N2 lymph nodes and the number of positive N2 lymph node stations had a significant influence on overall survival; clinical N status, histological type, the number of positive N2 lymph nodes and the number of positive N2 lymph node stations had a significant influence on locoregional control. Multivariate analysis demonstrated that the number of N2 positive lymph nodes (P= 0.017) was an independent factor for overall survival of stage ⅢA-N2 patients; the number of N2 positive lymph nodes (P=0.009) and histological type (P=0.005) were independent factors for locoregional recurrence. For left-sided lung cancer, the lymph node station failure sites were mostly in 2R, 4R, 5, 6 and 7, and

  14. Is high T-1 slope a significant risk factor for developing interlaminar bony fusion after cervical laminoplasty? A retrospective cohort study.

    Science.gov (United States)

    Oichi, Takeshi; Oshima, Yasushi; Oka, Hiroyuki; Taniguchi, Yuki; Chikuda, Hirotaka; Matsubayashi, Yoshitaka; Takeshita, Katsushi; Tanaka, Sakae

    2017-09-15

    in flexion, and had a significantly lower preoperative cervical ROM than those in the nonfusion group. A high T-1 slope was significantly more frequent in the fusion group. Multivariate analysis revealed that the significant risk factors for postoperative interlaminar bony fusion were high T-1 slope (odds ratio 4.81; p = 0.0015) and older age (odds ratio 1.05; p = 0.025). The Japanese Orthopaedic Association recovery rate in patients with interlaminar bony fusion did not differ significantly from those without bony fusion (45% vs 48%; p = 0.73). However, patients with bony fusion showed significantly reduced postoperative cervical ROM compared with those without bony fusion (50% vs 25%; p < 0.001). CONCLUSIONS High T-1 slope and older age were significant risk factors for developing interlaminar bony fusion after cervical laminoplasty in patients with CSM. Interlaminar bony fusion was associated with reduced postoperative cervical ROM, but it did not affect neurological outcomes.

  15. A novel, selective inhibitor of fibroblast growth factor receptors that shows a potent broad spectrum of antitumor activity in several tumor xenograft models.

    Science.gov (United States)

    Zhao, Genshi; Li, Wei-Ying; Chen, Daohong; Henry, James R; Li, Hong-Yu; Chen, Zhaogen; Zia-Ebrahimi, Mohammad; Bloem, Laura; Zhai, Yan; Huss, Karen; Peng, Sheng-Bin; McCann, Denis J

    2011-11-01

    The fibroblast growth factor receptors (FGFR) are tyrosine kinases that are present in many types of endothelial and tumor cells and play an important role in tumor cell growth, survival, and migration as well as in maintaining tumor angiogenesis. Overexpression of FGFRs or aberrant regulation of their activities has been implicated in many forms of human malignancies. Therefore, targeting FGFRs represents an attractive strategy for development of cancer treatment options by simultaneously inhibiting tumor cell growth, survival, and migration as well as tumor angiogenesis. Here, we describe a potent, selective, small-molecule FGFR inhibitor, (R)-(E)-2-(4-(2-(5-(1-(3,5-Dichloropyridin-4-yl)ethoxy)-1H-indazol-3yl)vinyl)-1H-pyrazol-1-yl)ethanol, designated as LY2874455. This molecule is active against all 4 FGFRs, with a similar potency in biochemical assays. It exhibits a potent activity against FGF/FGFR-mediated signaling in several cancer cell lines and shows an excellent broad spectrum of antitumor activity in several tumor xenograft models representing the major FGF/FGFR relevant tumor histologies including lung, gastric, and bladder cancers and multiple myeloma, and with a well-defined pharmacokinetic/pharmacodynamic relationship. LY2874455 also exhibits a 6- to 9-fold in vitro and in vivo selectivity on inhibition of FGF- over VEGF-mediated target signaling in mice. Furthermore, LY2874455 did not show VEGF receptor 2-mediated toxicities such as hypertension at efficacious doses. Currently, this molecule is being evaluated for its potential use in the clinic.

  16. The cultural dissemination significance of TV reality show--Tang Poetry as example%浅析电视真人秀节目的文化传播意义--以《唐诗风云会》为例

    Institute of Scientific and Technical Information of China (English)

    陈丹

    2016-01-01

    “真人秀”节目从西方电视媒体开始,经历的高度集中的发展,以惊人的速度在中国电视上发展壮大。在当今中国真人秀节目高度密集的情况下,如何利用真人秀节目进行对外文化传播,传播中国传统文化。文章通过对陕西卫视《唐诗风云会》节目的分析,总结了节目成功的基本要素。%The "reality show" program starts from the Western TV media, has experienced a high concentration of development, with amazing speed in the development of China's television. Chinese reality show highly intensive, how to use the reality show to carry out foreign cultural communication, spread traditional Chinese culture. This paper analyzes the Shaanxi satelite TV's "Tang poetry" program, summarizes the basic elements of the success of the program.

  17. The colorectal carcinoma prognosis factors: Significance of diagnosis delay Factores pronósticos en carcinoma colorrectal: Importancia de la demora diagnóstica

    Directory of Open Access Journals (Sweden)

    E. Gómez-Domínguez

    2006-05-01

    Full Text Available Introduction: detection of early-stage colorectal carcinoma (CRC -( Dukes' A or B- provides better survival rates in these patients. Thus, the effectiveness of screening programs in asymptomatic patients or of early diagnosis in symptomatic individuals has been postulated. The aim of this study was to establish whether a delay in diagnosis or other factors are related to CRC stage. Patients and methods: a retrospective study was performed on 96 patients with CRC. Age at diagnosis, gender distribution, intestinal disorders, diagnosis delay, primary sign and -regarding CRC- localization, stage (Dukes' and grade of differentiation (well differentiated; non-well differentiated; poorly differentiated were recorded. Results: diagnosis delay was 185 ± 190 days. Patients delay in obtaining a diagnosis was 119 ± 158 days. In 40% of patients CRC was diagnosed at an early stage (Dukes' A or B, and in 13% CRC was poorly differentiated. The only factor with an independent effect on Dukes' stage was tumor differentiation (p: 0.0012. Distal location was associated with less advanced tumors without statistical significance (p: 0.156. Conclusion: based on the presented data, a greater effort regarding screening programs for healthy people seems warranted, as improved survival has been demonstrated when diagnosis delay is reduced, particularly in patients with the highest mean delay.Introducción: el diagnóstico precoz del cáncer colorrectal (estadios A y B de Dukes consigue mejorar las tasas de supervivencia de estos pacientes. Con este objetivo se ha propuesto como estrategia acelerar el diagnóstico de enfermos sintomáticos o realizar cribados en enfermos asintomáticos. El objetivo de este trabajo es identificar los factores que influyen en la extensión tumoral del carcinoma colorrectal, especialmente la demora en el diagnóstico. Material y métodos: estudio prospectivo de una serie de 99 pacientes diagnosticados de carcinoma colorrectal en los que se

  18. Gefitinib, an epidermal growth factor receptor blockade agent, shows additional or synergistic effects on the radiosensitivity of esophageal cancer cells in vitro.

    Directory of Open Access Journals (Sweden)

    Taira,Naruto

    2006-02-01

    Full Text Available

    Human esophageal cancers have been shown to express high levels of epidermal growth factor receptor (EGFR and a relationship between high EGFR expression and local advance, the number of lymph node metastases, life expectancy, and sensitivity to chemo-radiotherapy has been demonstrated. We examined the use of gefitinib, an orally active EGFR-selective tyrosine kinase inhibitor, as a new strategy for treatment of esophageal carcinoma. The effects of gefitinib were evaluated in monotherapy and in combination with radiotherapy in human esophageal carcinoma cell lines. Gefitinib produced a dose-dependent inhibition of cellular proliferation in all of the 8 esophageal carcinoma cell lines examined, with IC50 values ranging from 5.7 microM to 36.9 microM. In combination, gefitinib and radiotherapy showed a synergistic effect in 2 human esophageal carcinoma cell lines and an additive effect in 5 cell lines. Western blotting demonstrated that gefitinib blocked activation of the EGFR-extracellular signal-regulated kinase (Erk pathway and the EGFR-phosphoinositide-3 kinase (PI3K-Akt pathway after irradiation. These results suggest that further evaluation of EGFR blockade as a treatment for esophageal cancer should be performed, and that radiotherapy combined with EGFR blockade may enhance the response of esophageal carcinoma to therapy.

  19. Hip dysplasia: a significant risk factor for the development of hip osteoarthritis. A cross-sectional survey

    DEFF Research Database (Denmark)

    Jacobsen, Steffen; Sonne-Holm, Stig

    2005-01-01

    OBJECTIVES: The aim of this cross-sectional survey of 2232 women and 1336 men (age range 20-91 yr) was to investigate individual risk factors for hip joint osteoarthritis (OA). METHODS: Standardized, weight-bearing pelvic radiographs were evaluated. Radiological hip joint OA was defined as minimu...

  20. A case report of motor neuron disease in a patient showing significant level of DDTs, HCHs and organophosphate metabolites in hair as well as levels of hexane and toluene in blood.

    Science.gov (United States)

    Kanavouras, Konstantinos; Tzatzarakis, Manolis N; Mastorodemos, Vasileios; Plaitakis, Andreas; Tsatsakis, Aristidis M

    2011-11-01

    Motor neuron disease is a devastating neurodegenerative condition, with the majority of sporadic, non-familial cases being of unknown etiology. Several epidemiological studies have suggested that occupational exposure to chemicals may be associated with disease pathogenesis. We report the case of a patient developing progressive motor neuron disease, who was chronically exposed to pesticides and organic solvents. The patient presented with leg spasticity and developed gradually clinical signs suggestive of amyotrophic lateral sclerosis, which was supported by the neurophysiologic and radiological findings. Our report is an evidence based case of combined exposure to organochlorine (DDTs), organophosphate pesticides (OPs) and organic solvents as confirmed by laboratory analysis in samples of blood and hair confirming systematic exposure. The concentration of non-specific dialkylphosphates metabolites (DAPs) of OPs in hair (dimethyphopshate (DMP) 1289.4 pg/mg and diethylphosphate (DEP) 709.4 pg/mg) and of DDTs (opDDE 484.0 pg/mg, ppDDE 526.6 pg/mg, opDDD 448.4 pg/mg, ppDDD+opDDT 259.9 pg/mg and ppDDT 573.7 pg/mg) were considerably significant. Toluene and n-hexane were also detected in blood on admission at hospital and quantified (1.23 and 0.87 μg/l, respectively), while 3 months after hospitalization blood testing was found negative for toluene and n-hexane and hair analysis was provided decrease levels of HCHs, DDTs and DAPs.

  1. Preoperative PET/CT FDG standardized uptake value of pelvic lymph nodes as a significant prognostic factor in patients with uterine cervical cancer

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Hyun Hoon [Seoul National University College of Medicine, Department of Obstetrics and Gynecology, Cancer Research Institute, Seoul (Korea, Republic of); Seoul National University College of Medicine, Department of Obstetrics and Gynecology, Seoul (Korea, Republic of); Cheon, Gi Jeong; Kang, Keon Wook [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul (Korea, Republic of); Kim, Jae Weon; Park, Noh-Hyun [Seoul National University College of Medicine, Department of Obstetrics and Gynecology, Cancer Research Institute, Seoul (Korea, Republic of); Song, Yong Sang [Seoul National University College of Medicine, Department of Obstetrics and Gynecology, Cancer Research Institute, Seoul (Korea, Republic of); Seoul National University, WCU Biomodulation Major, Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul (Korea, Republic of)

    2014-04-15

    Using integrated PET/CT, we evaluated the prognostic relevance in uterine cervical cancer of preoperative pelvic lymph node (LN) [{sup 18}F]FDG uptake. Patients with FIGO stage IB to IIA uterine cervical cancer were imaged with FDG PET/CT before radical surgery. We used Cox proportional hazards regression to examine the relationship between recurrence and the FDG maximum standardized uptake value (SUV{sub max}) in the pelvic LN (SUV{sub LN}) on PET/CT. Clinical data, treatment modalities, and results in 130 eligible patients were reviewed. The median postsurgical follow-up was 34 months (range 6 to 109 months). Receiver operating characteristic analysis identified SUV{sub LN} 2.36 as the most significant cut-off value for predicting recurrence. SUV{sub LN} was correlated with SUV{sub tumour} (P = 0.002), primary tumour size (P = 0.004), and parametrial invasion (P = 0.013). Univariate analyses showed significant associations between recurrence and SUV{sub LN} (P = 0.001), SUV{sub tumour} (P = 0.007), pelvic LN metastasis (P = 0.002), parametrial invasion (P < 0.001), primary tumour size (P = 0.007), suspected LN metastasis on MRI (P = 0.024), and FIGO stage (P = 0.026). Multivariate analysis identified SUV{sub LN} (P = 0.013, hazard ratio, HR, 4.447, 95 % confidence interval, CI, 1.379 - 14.343) and parametrial invasion (P = 0.013, HR 6.728, 95 % CI 1.497 - 30.235) as independent risk factors for recurrence. Patients with SUV{sub LN} ≥2.36 and SUV{sub LN} <2.36 differed significantly in terms of recurrence (HR 15.20, P < 0.001). Preoperative pelvic LN FDG uptake showed a strong significant association with uterine cervical cancer recurrence. (orig.)

  2. Impact of MET inhibition on small-cell lung cancer cells showing aberrant activation of the hepatocyte growth factor/MET pathway.

    Science.gov (United States)

    Taniguchi, Hirokazu; Yamada, Tadaaki; Takeuchi, Shinji; Arai, Sachiko; Fukuda, Koji; Sakamoto, Shuichi; Kawada, Manabu; Yamaguchi, Hiroyuki; Mukae, Hiroshi; Yano, Seiji

    2017-07-01

    Small-cell lung cancer (SCLC) accounts for approximately 15% of all lung cancers, and is characterized as extremely aggressive, often displaying rapid tumor growth and multiple organ metastases. In addition, the clinical outcome of SCLC patients is poor due to early relapse and acquired resistance to standard chemotherapy treatments. Hence, novel therapeutic strategies for the treatment of SCLC are urgently required. Accordingly, several molecular targeted therapies were evaluated in SCLC; however, they failed to improve the clinical outcome. The receptor tyrosine kinase MET is a receptor for hepatocyte growth factor (HGF), and aberrant activation of HGF/MET signaling is known as one of the crucial mechanisms enabling cancer progression and invasion. Here, we found that the HGF/MET signaling was aberrantly activated in chemoresistant or chemorelapsed SCLC cell lines (SBC-5, DMS273, and DMS273-G3H) by the secretion of HGF and/or MET copy number gain. A cell-based in vitro assay revealed that HGF/MET inhibition, induced either by MET inhibitors (crizotinib and golvatinib), or by siRNA-mediated knockdown of HGF or MET, constrained growth of chemoresistant SCLC cells through the inhibition of ERK and AKT signals. Furthermore, treatment with either crizotinib or golvatinib suppressed the systemic metastasis of SBC-5 cell tumors in natural killer cell-depleted SCID mice, predominantly through cell cycle arrest. These findings reveal the therapeutic potential of targeting the HGF/MET pathway for inhibition, to constrain tumor progression of SCLC cells showing aberrant activation of HGF/MET signaling. We suggest that it would be clinically valuable to further investigate HGF/MET-mediated signaling in SCLC cells. © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

  3. Whole genome association analysis shows that ACE is a risk factor for Alzheimer's disease and fails to replicate most candidates from Meta-analysis.

    Science.gov (United States)

    Webster, Jennifer; Reiman, Eric M; Zismann, Victoria L; Joshipura, Keta D; Pearson, John V; Hu-Lince, Diane; Huentelman, Matthew J; Craig, David W; Coon, Keith D; Beach, Thomas; Rohrer, Kristen C; Zhao, Alice S; Leung, Doris; Bryden, Leslie; Marlowe, Lauren; Kaleem, Mona; Mastroeni, Diego; Grover, Andrew; Rogers, Joseph; Heun, Reinhard; Jessen, Frank; Kölsch, Heike; Heward, Christopher B; Ravid, Rivka; Hutton, Michael L; Melquist, Stacey; Petersen, Ron C; Caselli, Richard J; Papassotiropoulos, Andreas; Stephan, Dietrich A; Hardy, John; Myers, Amanda

    2010-01-01

    For late onset Alzheimer's disease (LOAD), the only confirmed, genetic association is with the apolipoprotein E (APOE) locus on chromosome 19. Meta-analysis is often employed to sort the true associations from the false positives. LOAD research has the advantage of a continuously updated meta-analysis of candidate gene association studies in the web-based AlzGene database. The top 30 AlzGene loci on May 1(st), 2007 were investigated in our whole genome association data set consisting of 1411 LOAD cases and neuropathoiogicaiiy verified controls genotyped at 312,316 SNPs using the Affymetrix 500K Mapping Platform. Of the 30 "top AlzGenes", 32 SNPs in 24 genes had odds ratios (OR) whose 95% confidence intervals that did not include 1. Of these 32 SNPs, six were part of the Affymetrix 500K Mapping panel and another ten had proxies on the Affymetrix array that had >80% power to detect an association with α=0.001. Two of these 16 SNPs showed significant association with LOAD in our sample series. One was rs4420638 at the APOE locus (uncorrected p-value=4.58E-37) and the other was rs4293, located in the angiotensin converting enzyme (ACE) locus (uncorrected p-value=0.014). Since this result was nominally significant, but did not survive multiple testing correction for 16 independent tests, this association at rs4293 was verified in a geographically distinct German cohort (p-value=0.03). We present the results of our ACE replication aiongwith a discussion of the statistical limitations of multiple test corrections in whole genome studies.

  4. Prognostic significance of the co-expression of nucleophosmin and trefoil factor 3 in postoperative gastric cancer patients.

    Science.gov (United States)

    Li, Yong; Sun, Zhenqing; Liu, Kewei; Qiu, Wensheng; Yao, Ruyong; Feng, Tongtong; Xin, Chao; Yue, Lu

    2014-11-01

    Although a number of studies have indicated that the positive expression of nucleophosmin (NPM) and trefoil factor 3 (TFF3) is associated with oncogenesis and poor prognosis in several tumor types, the prognostic value of the co-expression of NPM and TFF3 in gastric cancer (GC) has not been fully elucidated. Therefore, in this study, we aimed to investigate the role of NPM and TFF3 in GC and determine their prognostic value. We retrospectively reviewed 108 patients who had undergone radical gastric tumor resection. The expression of NPM and TFF3 was detected by immunohistochemistry and the association of NPM and TFF3 with clinicopathological characteristics was investigated using the Chi-square test. Furthermore, univariate and multivariate analyses were conducted to determine the prognostic value of these markers. Of the 108 samples, NPM was positive in 57 (53%) and TFF3 was positive in 54 samples (50%). The positive expression of NPM was correlated with advanced tumor stage and recurrence (P=0.0333 and PTFF3 was associated with larger tumor size (P=0.0005), poor differentiation (P=0.0435), lymph node metastasis (P=0.0116), advanced tumor stage (P=0.0244) and recurrence (P=0.0116). The univariate analysis revealed that the expression of NPM, the expression of TFF3 and the co-expression of the two were associated with poor survival (P=0.0004, 0.0028 and 0.0020, respectively). By multivariate analysis, all three factors were identified as independent prognostic factors in postoperative GC patients (hazard ratio = 1.970, 2.021 and 2.339, respectively). In conclusion, the expression of NPM and TFF3 and, particularly, the co-expression of the two, may serve as independent prognostic factors in postoperative GC patients.

  5. Growth-differentiation factor-8 (GDF-8 in the uterus: its identification and functional significance in the golden hamster

    Directory of Open Access Journals (Sweden)

    O Wai Sum

    2009-11-01

    Full Text Available Abstract Transforming growth factor-beta superfamily regulates many aspects of reproduction in the female. We identified a novel member of this family, growth-differentiation factor 8 (GDF-8 in the 72 h post coital uterine fluid of the golden hamster by proteomic techniques. Uterine GDF-8 mRNA decreased as pregnancy progressed while its active protein peaked at 72 h post coitus (hpc and thereafter stayed at a lower level. At 72 hpc, the GDF-8 transcript was localized to the endometrial epithelium while its protein accumulated in the stroma. Exogenous GDF-8 slowed down proliferation of primary cultures of uterine smooth muscle cells (SMC and endometrial epithelial cells (EEC. In addition, GDF-8 attenuated the release of LIF (leukaemia inhibiting factor by EEC. As for the embryo in culture, GDF-8 promoted proliferation of the trophotoderm (TM and hatching but discouraged attachment. Our study suggests that GDF-8 could regulate the behavior of preimplantation embryos and fine-tune the physiology of uterine environment during pregnancy.

  6. Growth-differentiation factor-8 (GDF-8) in the uterus: its identification and functional significance in the golden hamster.

    Science.gov (United States)

    Wong, Chun Lung; Huang, Ya Yu; Ho, Wing Kei; Poon, Hong Kit; Cheung, Pui Lai; O, Wai Sum; Chow, Pak Ham

    2009-11-25

    Transforming growth factor-beta superfamily regulates many aspects of reproduction in the female. We identified a novel member of this family, growth-differentiation factor 8 (GDF-8) in the 72 h post coital uterine fluid of the golden hamster by proteomic techniques. Uterine GDF-8 mRNA decreased as pregnancy progressed while its active protein peaked at 72 h post coitus (hpc) and thereafter stayed at a lower level. At 72 hpc, the GDF-8 transcript was localized to the endometrial epithelium while its protein accumulated in the stroma. Exogenous GDF-8 slowed down proliferation of primary cultures of uterine smooth muscle cells (SMC) and endometrial epithelial cells (EEC). In addition, GDF-8 attenuated the release of LIF (leukaemia inhibiting factor) by EEC. As for the embryo in culture, GDF-8 promoted proliferation of the trophotoderm (TM) and hatching but discouraged attachment. Our study suggests that GDF-8 could regulate the behavior of preimplantation embryos and fine-tune the physiology of uterine environment during pregnancy.

  7. Significant risk factors for occurrence of cancer after renal transplantation: a single center cohort study of 1265 cases.

    Science.gov (United States)

    Bichari, W; Bartiromo, M; Mohey, H; Afiani, A; Burnot, A; Maillard, N; Sauron, C; Thibaudin, D; Mehdi, M; Mariat, C; Alamartine, E; Berthoux, F

    2009-03-01

    Occurrence of cancer after renal transplantation remains a major problem, and the second cause of death. We performed a retrospective analysis of first cancer, first skin cancer, and first organ cancer (including posttransplant lymphoproliferative disease [PTLD]) among 1265 cases from 1979 to 2006. The occurrence of cancer was clearly a time-dependent event justifiying the use of Kaplan-Meier survival and Cox regression methods. The 10-year cumulative incidences of first cancer, first skin cancer, and first organ cancer were 24.6%, 14.5%, and 14.5%, respectively. Recipient age was a major, independent risk factor for the 3 endpoints with a 6% increased relative risk for each year increment (P < .0001). Female gender was also a major, independent risk factor, but only for skin cancer (P = .0002). We could not demonstrate any difference between the immunosuppressive drugs used for induction or maintenance therapy, especially between antithymocyte globulin (ATG) vs anti-CD25, cyclosporine vs tacrolimus, and azathioprine vs mycophenolate mofetil. Large cohorts are needed with strict stratifications for recipient age and gender to detect any difference, if any, among the drugs.

  8. "We Are in Transylvania, and Transylvania Is Not England": Location as a Significant Factor in International School Micropolitics

    Science.gov (United States)

    Caffyn, Richard

    2010-01-01

    This article looks at research undertaken into two international schools focusing on micropolitics and the importance of school location. Location appears to have a significant impact on the behaviour of those associated with each school, such as reactions to physical environment, local culture and clientele. These affect the micropolitical…

  9. Identifying factors significant to continuity in basic palliative hospital care-from the perspective of patients with advanced cancer

    DEFF Research Database (Denmark)

    Soelver, Lisbeth; Rydahl-Hansen, Susan; Oestergaard, Birte;

    2014-01-01

    . The interviews were based on selected problems that patients found significant. The results are distilled into the core category disheartening interactions and four categories: falling outside the professional framework, not being seen as a person, unidentified/unmet need for guidance and involvement...

  10. Determination of vascular endothelial growth factor (VEGF) in circulating blood: significance of VEGF in various leucocytes and platelets

    DEFF Research Database (Denmark)

    Werther, K; Christensen, Ib Jarle; Nielsen, Hans Jørgen

    2002-01-01

    . In corresponding blood samples, automated complete blood count was performed, and the number of each cell type was correlated to VEGF concentrations in plasma, serum and lysed whole blood. Finally, the impact of increasing clotting time on the release of VEGF to serum was analysed. RESULTS: Isolated neutrophils......AIM: The sources of increased vascular endothelial growth factor (VEGF) concentrations in peripheral blood from cancer patients are not known in detail. The aim of the present study was to evaluate correlations between the VEGF content in isolated leucocyte subpopulations and VEGF concentrations...... in plasma, serum and lysed whole blood. METHODS: In 51 colorectal cancer (CRC) patients, circulating T-lymphocytes, B-lymphocytes, monocytes, and granulocytes were isolated by means of immunomagnetic separation. Subsequently, the isolated cells were lysed and VEGF contents in the lysates were determined...

  11. Central Hypothyroidism and Its Replacement Have a Significant Influence on Cardiovascular Risk Factors in Adult Hypopituitary Patients

    DEFF Research Database (Denmark)

    Klose, M; Marina, Djordje; Hartoft-Nielsen, M-L

    2013-01-01

    Context: Thyroid dysfunction may have detrimental effects on patient outcomes. Few studies have assessed this issue in patients with secondary hypothyroidism. Objective: Our objective was to test the hypothesis that thyroid hormone status has an impact on cardiovascular risk factors in adult...... collected at baseline and a median 4.1 years after commencement of GH. Results: At baseline, fT4 was negatively associated with BMI and waist circumference, but positively with high-density lipoprotein, independent of age, gender, and IGF-I (SD score). Only first-tertile TSH-deficient patients had higher...... cholesterol persisted only in first-tertile TSH-deficient patients. Conclusion: This single-center study over a 20-year period has strengthened the importance of improved awareness of thyroid status and optimal thyroid replacement of hypopituitary patients to reduce cardiovascular risks in hypopituitary...

  12. [Recording cervical and ocular vestibular evoked myogenic potentials. Part 2: influencing factors, evaluation of findings and clinical significance].

    Science.gov (United States)

    Walther, L E; Hörmann, K; Pfaar, O

    2010-11-01

    VEMP measurements are subject to various influencing factors: patient age, threshold, sound intensity and frequency. Using air (AC) and bone conduction (BC) the vestibular receptors and afferents of the otolith organs can be activated to varying degrees. Recordings of cervical (cVEMP) and ocular VEMP (oVEMP) are clinically possible. AC-cVEMP are primarily an indicator of the sacculocollic reflex pathway. Together with findings on the vestibuloocular reflex (VOR) and complimentary otolith tests, VEMP enable otolith function analysis of each side separately. In addition, the distinction between combined or isolated canal and otolith dysfunction in terms of subtyping and patterns of damage in mono- and bilateral disorders, such as vestibular neuritis or bilateral vestibulopathy, is possible. Moreover, VEMP is relevant in terms of prognostic and therapeutic considerations as well as expert assessments.

  13. DEVELOPMENT OF QUESTIONNAIRE FOR MEASURING FACTORS OF SIGNIFICANCE OF AN ELDERLY PERSON AS ANOTHER FOR THE YOUNGER GENERATION

    OpenAIRE

    KHALINA Natalia V.; Stanislav A. POZDNIAKOV

    2015-01-01

    Social and political processes of the present time, actualize in the minds of the younger generation of Russians importance for the development of the country. But it would be wrong to assume no significant experience in the elderly and to consider identity apart from family ties. Relevance to this key acts the process of intergenerational transmission, when the younger generation can assimilate the experience of close, meaningful elderly. The paper drew attention to the dedicated A.V. Petrov...

  14. Expression and significance of fat mass and obesity associated gene and forkhead transcription factor O1 in non-alcoholic fatty liver disease

    Institute of Scientific and Technical Information of China (English)

    Zhang Jielei; Li Shan; Li Jingyi; Han Chao; Wang Zhifang; Li Chong; Wang Xiaoman

    2014-01-01

    Background Non-alcoholic fatty liver disease (NAFLD) is a complex disorder and has been closely linked to obesity.The fat mass and obesity-associated (FTO) gene is a newly discovered gene related to obesity,which enhances oxidative stress and tipogenesis in NAFLD.The forkhead transcription factor O1 (FoxO1) is another important gene involved in NAFLD,which causes lipid disorders when insulin resistance appears in the liver.However,the interactions between FTO and FoxO1 during the pathogenesis of NAFLD have not been fully elucidated.This study was designed to identify the relationship between these two factors that are involved in the development of NAFLD.Methods This study includes two parts referred to as animal and cell experiments.Twelve female SPF C57BL/6 mice were fed a high-fat diet to serve as an NAFLD animal model.Aspartate aminotransferase (AST),alanine aminotransferase (ALT),total triglyceride (TG),total cholesterol (TC),alkaline phosphatase (ALP),high-density lipoprotein (HDL),and low-density lipoprotein (LDL) were measured.Immunohistochemical analysis was used to detect the expression and histological localization of FTO,FoxO1,and adenosine monophosphate (AMP)-activated protein kinase (AMPK).The L02 cells were exposed to high fat for 24,48,or 72 hours.Oil red O staining was used to detect intracellular lipid droplets.Reverse transcription-polymerase chain reaction was used for analyzing the levels of FTO and FoxO1 mRNA.Results At the end of 10 weeks,ALP,ALT,AST,and LDL were significantly increased (P <0.01),while TC and TG were also significantly higher (P <0.05).In addition,HDL was significantly decreased (P <0.05).The FTO and FoxO1 proteins were weakly expressed in the control group,but both FTO and FoxO1 were expressed significantly higher (P <0.01) in the experimental group,and the expression of the two factors was significantly correlated.AMPK in the high-fat group showed a low level of correlation with FTO,but not with FoxO1.Oil Red O

  15. [Expression and clinical significance of kisspeptin-1, matrix metalloproteinase-2 and vascular endothelial growth factor in tissue of colon cancer].

    Science.gov (United States)

    Wang, Wenhui; Qi, Yuanling; Xu, Qian; Ren, Haipeng

    2016-03-01

    To detect the expression of kisspeptin-1 (KISS-1), matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) in the tissue of colon cancer, and analyze the relativity between KISS-1, MMP-2, VEGF and pathological characteristics of colon cancer. A total of 60 colon cancer patients and 60 patients with benign colorectal disease who received surgical treatment in our hospital from January 2009 to June 2010 were selected as observation group and control group respectively. The cancer tissue samples and excision samples collected from them were used to detect KISS-1, MMP-2 and VEGF with immunohistochemistry. The positive rates of KISS-1, MMP-2 and VEGF were 31.7%, 58.3% and 78.3% in observation group, and 73.3%, 16.7% and 33.3% in control group. The positive rate of KISS-1 in observation group was lower than that in control group (χ(2)=23.489, PTNM stage of colon cancer (χ(2)=8.997, P=0.011; χ(2)=6.163, P=0.013; χ(2)=8.519, P=0.014; χ(2)=9.160, P=0.002; χ(2)=16.577, Pcolon cancer and provide evidence for clinical diagnosis and prognosis prediction by detecting KISS-1, MMP-2 and VEGF.

  16. Pigment epithelium-derived factor: clinical significance in estrogen-dependent tissues and its potential in cancer therapy

    Science.gov (United States)

    Franco-Chuaire, María Liliana; Ramírez-Clavijo, Sandra; Chuaire-Noack, Lilian

    2015-01-01

    Pigment epithelium-derived factor (PEDF) is a glycoprotein that belongs to the family of non-inhibitory serpins. The broad spectrum of PEDF biological activity is evident when considering its effects in promoting cell survival and proliferation, as well as its antiangiogenic, antitumor, and anti-metastatic properties. Although the structural domains of the PEDF gene that mediate such diverse effects and their mechanisms of action have not been completely elucidated, there is a large body of evidence describing their diverse range of activities; this evidence combined with the regulation of PEDF expression by sex steroids and their receptors have led to the idea that PEDF is not only a diagnostic and prognostic marker for certain diseases such as cancer, but is also a potential therapeutic target. In this manner, this paper aims to generally review the regulation of PEDF expression and PEDF interactions, as well as the findings that relate PEDF to the role of estrogens and estrogen receptors. In addition, this manuscript will review major advances toward potential therapeutic applications of PEDF. PMID:26523216

  17. Pigment epithelium-derived factor: clinical significance in estrogen-dependent tissues and its potential in cancer therapy

    Directory of Open Access Journals (Sweden)

    María Liliana Franco-Chuaire

    2015-09-01

    Full Text Available Pigment epithelium-derived factor (PEDF is a glycoprotein that belongs to the family of non-inhibitory serpins. The broad spectrum of PEDF biological activity is evident when considering its effects in promoting cell survival and proliferation, as well as its antiangiogenic, antitumor, and anti-metastatic properties. Although the structural domains of the PEDF gene that mediate such diverse effects and their mechanisms of action have not been completely elucidated, there is a large body of evidence describing their diverse range of activities; this evidence combined with the regulation of PEDF expression by sex steroids and their receptors have led to the idea that PEDF is not only a diagnostic and prognostic marker for certain diseases such as cancer, but is also a potential therapeutic target. In this manner, this paper aims to generally review the regulation of PEDF expression and PEDF interactions, as well as the findings that relate PEDF to the role of estrogens and estrogen receptors. In addition, this manuscript will review major advances toward potential therapeutic applications of PEDF.

  18. Preoperative thrombocytosis is a significant unfavorable prognostic factor for patients with resectable non-small cell lung cancer.

    Science.gov (United States)

    Kim, Miso; Chang, Hyun; Yang, Hee Chul; Kim, Yu Jung; Lee, Choon-Taek; Lee, Jae-Ho; Jheon, Sanghoon; Kim, Kwhanmien; Chung, Jin-Haeng; Lee, Jong Seok

    2014-02-12

    Previous studies have reported that pretreatment thrombocytosis is associated with poor outcomes in several cancer types. This study was designed to evaluate the prognostic significance of preoperative thrombocytosis in patients with non-small cell lung cancer (NSCLC) who undergo surgery. We retrospectively reviewed the records of 199 patients who underwent R0 resection for NSCLC between May 2003 and July 2006 at Seoul National University Bundang Hospital, Seongnam, Korea. The frequency of preoperative thrombocytosis was 7.5% (15/199). Patients with preoperative thrombocytosis had shorter overall survival (OS, P = 0.003) and disease-free survival (DFS, P = 0.005) than those without thrombocytosis. In multivariable analysis, patients with preoperative thrombocytosis had a significantly greater risk of death and recurrence than those without preoperative thrombocytosis (risk of death: hazard ratio (HR) 2.98, 95% confidence interval (CI) 1.39 to 6.37, P = 0.005; risk of recurrence: HR 2.47, 95% CI 1.22 to 5.01, P = 0.012). A tendency towards a shorter OS and DFS was observed in three patients with persistent thrombocytosis during the follow-up period when compared with those of patients who recovered from thrombocytosis after surgery. Preoperative thrombocytosis was valuable for predicting the prognosis of patients with NSCLC. Special attention should be paid to patients with preoperative and postoperative thrombocytosis.

  19. Expression of nuclear factor-kappa B in hepatic tissue and its significance in rats after repeated and sustained +10Gz exposure

    Directory of Open Access Journals (Sweden)

    Lei LIU

    2015-07-01

    Full Text Available Objective To observe the injury to liver cells, and to investigate the changes in nuclear factor-kappa B (NF-κB in the liver tissue after exposure to positive ten acceleration (+10Gz. Methods Twenty-four adult male Wistar rats were randomly assigned to four groups: blank control, exposure groups A, B, and C. The rats of three exposure groups were subjected to centrifugal force of +10Gz for 0.5, 24, and 48h respectively, which was carried out in an Air Force Institute of Aviation Medicine. Plasma aspartate aminotransferase (AST and alanine aminotransferase (ALT were determined. Liver tissue was harvested for immunohistochemistry, and Western blotting was performed for expression of NF-κB. Results After +10 Gz exposure, rats of the group A showed shaggy hair, apathetic to food, and laid still, while group B rats showed shaggy hair, took a little food with lowering of activity level. The rats in group C showed normal activities. Both the levels of serum AST and ALT were elevated, and they were higher in group B than that of groups A and C (P0.05. Western blotting and immunohistochemical staining showed that the NF-κB expression levels were significantly higher in exposure groups than in control group (P0.05. Immunohistochemical examination revealed NF-κBpositive products were localized predominantly in the hepatocytes, and also in the inflammatory cells and Kupffer cells. These products could be divided into three types: cytosolic type, karyotype, and nuclear-cytoplasm type, and they existed alone or in combination. Conclusion Exposure to +10Gz, especially in the early stage, acts as a stress to induce the activation of NF-κB, suggesting that NF-κB plays an important role in the cascade reactions and adaptation to the acceleration stress. DOI: 10.11855/j.issn.0577-7402.2015.07.17

  20. Caregiving and Developmental Factors Differentiating Young At-Risk Urban Children Showing Resilient Versus Stress-Affected Outcomes: A Replication and Extension.

    Science.gov (United States)

    Wyman, Peter A.; And Others

    1999-01-01

    Tested hypotheses from an organizational-developmental model for childhood resilience among 7- to 9-year olds. Found that caregiving factors and early development differentiated children with resilient and stress-affected adaptations. Variables reflecting emotionally responsive, competent parenting were direct, proximal predictors of resilience…

  1. Persistent depression is a significant risk factor for the development of arteriosclerosis in middle-aged Japanese male subjects.

    Science.gov (United States)

    Satoh, Hiroki; Fujii, Satoshi; Tsutsui, Hiroyuki

    2015-01-01

    Depression often coexists with hypertension and various cardiovascular diseases. However, the relationship between depression and the development of arteriosclerosis has not been fully established. We assessed depression and brachial-ankle pulse wave velocity (baPWV) in 828 middle-aged Japanese male subjects at baseline and during 3 years of follow-up. Depression was assessed using Center for Epidemiological Studies-Depression questionnaires, and the study subjects were divided into the following three groups: persistent depression, transient depression and no depression. The number (%) of subjects with persistent, transient and no depression were 104 (12.6), 76 (9.2) and 648 (78.2), respectively. Blood pressure and baPWV did not differ among the three groups at baseline. The changes in the baPWV values (⊿baPWV) correlated significantly and positively with age, body mass index, baseline systolic blood pressure and persistent depression (r = 0.32, P arteriosclerosis in middle-aged Japanese male subjects.

  2. Nonpermissive HLA-DPB1 disparity is a significant independent risk factor for mortality after unrelated hematopoietic stem cell transplantation.

    Science.gov (United States)

    Crocchiolo, Roberto; Zino, Elisabetta; Vago, Luca; Oneto, Rosi; Bruno, Barbara; Pollichieni, Simona; Sacchi, Nicoletta; Sormani, Maria Pia; Marcon, Jessica; Lamparelli, Teresa; Fanin, Renato; Garbarino, Lucia; Miotti, Valeria; Bandini, Giuseppe; Bosi, Alberto; Ciceri, Fabio; Bacigalupo, Andrea; Fleischhauer, Katharina

    2009-08-13

    The importance of donor-recipient human leukocyte antigen (HLA)-DPB1 matching for the clinical outcome of unrelated hematopoietic stem cell transplantation (HSCT) is controversial. We have previously described an algorithm for nonpermissive HLA-DPB1 disparities involving HLA-DPB1*0901,*1001,*1701,*0301,*1401,*4501, based on T-cell alloreactivity patterns. By revisiting the immunogenicity of HLA-DPB1*02, a modified algorithm was developed and retrospectively tested in 621 unrelated HSCTs facilitated through the Italian Registry for oncohematologic adult patients. The modified algorithm proved to be markedly more predictive of outcome than the original one, with significantly higher Kaplan-Meier probabilities of 2-year survival in permissive compared with nonpermissive transplantations (55% vs 39%, P = .005). This was the result of increased adjusted hazards of nonrelapse mortality (hazard ratio [HR] = 1.74; confidence interval [CI], 1.19-2.53; P = .004) but not of relapse (HR = 1.02; CI, 0.73-1.42; P = .92). The increase in the hazards of overall mortality by nonpermissive HLA-DPB1 disparity was similar in 10 of 10 (HR = 2.12; CI, 1.23-3.64; P = .006) and 9 of 10 allele-matched transplantations (HR = 2.21; CI, 1.28-3.80; P = .004), both in early-stage and in advanced-stage disease. These data call for revisiting current HLA matching strategies for unrelated HSCT, suggesting that searches should be directed up-front toward identification of HLA-DPB1 permissive, 10 of 10 or 9 of 10 matched donors.

  3. Significance of peak height velocity as a predictive factor for curve progression in patients with idiopathic scoliosis

    Science.gov (United States)

    2015-01-01

    progression in patients with IS. Conclusions These findings indicate that 31.5 degrees of spinal curvature when patients are at PHV is a significant predictive indicator for progression of the curve to a magnitude requiring surgery. We suggest that the curve-progression risk assessment in patients with IS should include PHV, along with measures of skeletal and non-skeletal maturities. PMID:25815057

  4. Factores significativos del rendimiento excelente: PISA y otros estudios Fatores significativos no rendimento excelente: PISA e outros estudos Significant factors in the yield excellent: PISA and other studies

    Directory of Open Access Journals (Sweden)

    Carmen Jiménez Fernández

    2012-12-01

    Full Text Available Se centra en tres investigaciones sobre los alumnos con rendimiento excelente. El estudio de PISA Top of de class, que analiza los factores subyacentes al rendimiento excelente de los alumnos de 15-16 años; el estudio de una muestra de alumnos españoles que han obtenido Premio Extraordinario de Bachillerato (PEB y un tercer estudio sobre alumnos que han obtenido Beca de Excelencia (BEpara estudiar en la universidad de Madrid. Se concluye que aunque la procedencia social no es determinante, la mayor parte de los alumnos con rendimiento excelente proceden de ambientes culturalmente favorecidos si bien el centro escolar ejerce mayor influencia que la clase social; la cantidad de horas semanales que el currículo dedica a la enseñanza de las materias fundamentales y la cantidad de horas que el alumno dedica al estudio son factores importantes del rendimiento excelente; los centros con mejor rendimiento atraen más a los alumnos con rendimiento excelente y, a su vez, hay más alumnos con rendimiento excelente en los centros más elitistas; en el estudio de PISA no hay diferencias de género; en los estudios sobre alumnos con PEB y BE persisten reductos sexistas.O presente trabalho se centra em três pesquisas sobre alunos com rendimento excelente. O estudo de PISA Top of de class, que analisa os fatores subjacentes ao rendimento excelente de alunos entre 15 e16 anos; o estudo de uma amostra de alunos espanhóis que obtiveram o Premio Extraordinário de Ensino Médio e um terceiro estudo sobre alunos que obtiveram Bolsa de Excelência para estudar na Universidade de Madrid. Chega-se à conclusão que embora a procedência social não seja determinante, a maior parte dos alunos com rendimento excelente procedem de ambientes culturalmente favorecidos, ainda que o centro escolar exerce maior influência que a classe social; a quantidade de horas semanais que o currículo dedica ao ensino das disciplinas fundamentais e a quantidade de horas que o aluno

  5. Caregiving and developmental factors differentiating young at-risk urban children showing resilient versus stress-affected outcomes: a replication and extension.

    Science.gov (United States)

    Wyman, P A; Cowen, E L; Work, W C; Hoyt-Meyers, L; Magnus, K B; Fagen, D B

    1999-01-01

    This study tested hypotheses from an organizational-developmental model for childhood resilience. In this model resilience reflects a child's mastery of age-salient objectives, in the face of substantial adversity, by drawing on internal and external resources that enhance processes of adaptation specific to each developmental stage. Interviews were conducted with parents of 122 7- to 9-year-old urban children exposed to multiple risk factors, 69 classified as resilient and 53 as maladjusted. Consistent with predictions generated by the model: (1) characteristics of a child's caregiving system and early development differentiated children with resilient and stress-affected adaptations; and (2) variables reflecting emotionally responsive, competent parenting were direct, proximal predictors of resilient status and mediators of other caregiver resources such as education, mental health, and relational history. Identified predictors of resilient status, including competent parenting and caregiver psychosocial resources, largely replicated findings from a prior study with sociodemographically comparable 9- to 12-year-old children.

  6. Arterial and venous thrombosis in patients with monoclonal gammopathy of undetermined significance: incidence and risk factors in a cohort of 1491 patients.

    Science.gov (United States)

    Za, Tommaso; De Stefano, Valerio; Rossi, Elena; Petrucci, Maria Teresa; Andriani, Alessandro; Annino, Luciana; Cimino, Giuseppe; Caravita, Tommaso; Pisani, Francesco; Ciminello, Angela; Torelli, Fabio; Villivà, Nicoletta; Bongarzoni, Velia; Rago, Angela; Betti, Silvia; Levi, Anna; Felici, Stefano; Gentilini, Fabiana; Calabrese, Elisabetta; Leone, Giuseppe

    2013-03-01

    Monoclonal gammopathy of undetermined significance (MGUS) has been associated with an increased risk of thrombosis. We carried out a retrospective multicentre cohort study on 1491 patients with MGUS. In 49 patients (3.3%) MGUS was diagnosed after a thrombotic event. Follow-up details for a period of at least 12 months after diagnosis of MGUS were obtained in 1238 patients who had no recent history of thrombosis (thrombosis, with an incidence of 2.5 arterial events and 1.9 venous events per 1000 patient-years. Multivariate analysis showed increased risks of arterial thrombosis in patients with cardiovascular risk factors [hazard ratio (HR) 4.92, 95%confidence interval (CI) 1.42-17.04], and of venous thrombosis in patients with a serum monoclonal (M)-protein level >16 g/l at diagnosis (HR 3.08, 95%CI 1.01-9.36). No thrombosis was recorded in patients who developed multiple myeloma (n = 50) or other neoplastic diseases (n = 21). The incidence of arterial or venous thrombosis in patients with MGUS did not increase relative to that reported in the general population for similarly aged members. Finally, the risk of venous thrombosis did increase when the M-protein concentration exceeded >16 g/l.

  7. Significant effects of birth-related biological factors on pre-adolescent nutritional status among rural Sundanese in West Java, Indonesia.

    Science.gov (United States)

    Sekiyama, Makiko; Ohtsuka, Ryutaro

    2005-07-01

    The Sundanese inhabiting West Java, the second largest ethnic group in Indonesia, are characterized by a high prevalence of child malnutrition, together with high fertility. Based on an anthropometric measurement and interview survey of 310 children aged 5-12 years in a rural Sundanese village, this study examined the relative significance of the effects of eight biological, eight socioeconomic status (SES) and four health behavioural factors on their Z scores for height-for-age (HAZ) and weight-for-age (WAZ) in particular. Three biological factors, i.e. birth interval, birth weight and mother's body weight, and one SES factor, i.e. mother's occupation, were selected as the predictors of the two Z scores by regression analysis, indicating more significant effects of the biological factors than the other factors. This pattern is judged to occur in less-developed and high-fertility populations. Since these two Z scores were worse in the subject children than in the under-5-year-old children from the same village, more attention should be paid to the long-lasting effects of birth-related biological factors up to pre-adolescent ages, as an insufficient nutritional status tends to damage growth and health in adolescence and adulthood.

  8. Specific complex of non-radiation risk factors for socially significant pathologies could affect the liquidators of Chernobyl nuclear power plant accident

    Directory of Open Access Journals (Sweden)

    Koterov A.N.

    2014-12-01

    Full Text Available The review considers the complex of non-radiation factors that could affect the liquidators of the Chernobyl accident: the demographic, social and professional group heterogeneity to warrant differentiation of risk, the effects of heavy metals, 'hot particles', chemicals, psychogenic stress, social dislocation in the post-perestroika period, alcohol abuse, smoking, and the effect of screening. All these factors tend to have a significant intensity, unlike the radiation exposure for the majority of subjects. It is concluded that the increased frequency and severity of some large socially significant pathologies in contingent liquidators may be due to a unique set of predominantly non-radiation factors associated, however, with a particular radiation accident.

  9. Administrators' Perceptions of Factors Related to Student Retention at Colleges with a Significant Black Student Enrollment Affiliated with the Association for Biblical Higher Education

    Science.gov (United States)

    Wilson, Wesley B.

    2013-01-01

    This study described and explored the factors perceived as relevant to student retention by administrators at colleges and universities with significant Black student populations. The sample was 31 institutions affiliated with the Association for Biblical Higher Education (ABHE) that had Black student enrollment of 20% or more. The study sought to…

  10. Expression and clinical significance of vascular endothelial growth factor-C and nm23-H1 in stage Ⅱ and Ⅲ colorectal carcinomas

    Institute of Scientific and Technical Information of China (English)

    耿倩倩

    2013-01-01

    Objective To discuss the expression and clinical significance of VEGF-C and nm23-H1 in stageⅡandⅢcolorectal carcinomas.Methods SP immunohistochemical staining was employed to determine the expression of vascular endothelial growth factor-C (VEGF-C) and nm23-H1 in the tumor tissues of 110 cases of stageⅡ

  11. Numerical study identifying the factors causing the significant underestimation of the specific discharge estimated using the modified integral pumping test method in a laboratory experiment.

    Science.gov (United States)

    Sun, Kerang

    2015-09-01

    A three-dimensional finite element model is constructed to simulate the experimental conditions presented in a paper published in this journal [Goltz et al., 2009. Validation of two innovative methods to measure contaminant mass flux in groundwater. Journal of Contaminant Hydrology 106 (2009) 51-61] where the modified integral pumping test (MIPT) method was found to significantly underestimate the specific discharge in an artificial aquifer. The numerical model closely replicates the experimental configuration with explicit representation of the pumping well column and skin, allowing for the model to simulate the wellbore flow in the pumping well as an integral part of the porous media flow in the aquifer using the equivalent hydraulic conductivity approach. The equivalent hydraulic conductivity is used to account for head losses due to friction within the wellbore of the pumping well. Applying the MIPT method on the model simulated piezometric heads resulted in a specific discharge that underestimates the true specific discharge in the experimental aquifer by 18.8%, compared with the 57% underestimation of mass flux by the experiment reported by Goltz et al. (2009). Alternative simulation shows that the numerical model is capable of approximately replicating the experiment results when the equivalent hydraulic conductivity is reduced by an order of magnitude, suggesting that the accuracy of the MIPT estimation could be improved by expanding the physical meaning of the equivalent hydraulic conductivity to account for other factors such as orifice losses in addition to frictional losses within the wellbore. Numerical experiments also show that when applying the MIPT method to estimate hydraulic parameters, use of depth-integrated piezometric head instead of the head near the pump intake can reduce the estimation error resulting from well losses, but not the error associated with the well not being fully screened.

  12. The chimeric repressor version of an Ethylene Response Factor (ERF) family member, Sl-ERF.B3, shows contrasting effects on tomato fruit ripening.

    Science.gov (United States)

    Liu, Mingchun; Diretto, Gianfranco; Pirrello, Julien; Roustan, Jean-Paul; Li, Zhengguo; Giuliano, Giovanni; Regad, Farid; Bouzayen, Mondher

    2014-07-01

    Fruit ripening involves a complex interplay between ethylene and ripening-associated transcriptional regulators. Ethylene Response Factors (ERFs) are downstream components of ethylene signaling, known to regulate the expression of ethylene-responsive genes. Although fruit ripening is an ethylene-regulated process, the role of ERFs remains poorly understood. The role of Sl-ERF.B3 in tomato (Solanum lycopersicum) fruit maturation and ripening is addressed here using a chimeric dominant repressor version (ERF.B3-SRDX). Over-expression of ERF.B3-SRDX results in a dramatic delay of the onset of ripening, enhanced climacteric ethylene production and fruit softening, and reduced pigment accumulation. Consistently, genes involved in ethylene biosynthesis and in softening are up-regulated and those of carotenoid biosynthesis are down-regulated. Moreover, the expression of ripening regulators, such as RIN, NOR, CNR and HB-1, is stimulated in ERF.B3-SRDX dominant repressor fruits and the expression pattern of a number of ERFs is severely altered. The data suggest the existence of a complex network enabling interconnection between ERF genes which may account for the pleiotropic alterations in fruit maturation and ripening. Overall, the study sheds new light on the role of Sl-ERF.B3 in the transcriptional network controlling the ripening process and uncovers a means towards uncoupling some of the main ripening-associated processes.

  13. Use of a serum-free epidermal culture model to show deleterious effects of epidermal growth factor on morphogenesis and differentiation.

    Science.gov (United States)

    Chen, C S; Lavker, R M; Rodeck, U; Risse, B; Jensen, P J

    1995-01-01

    The presence of serum has limited the utility of many culture models for the study of cytokine effects because its complexity and variability can confound the interpretation of data. In the present study, a serum-free skin co-culture model was used to investigate the effect of exogenous epidermal growth factor (EGF) on epidermal proliferation and differentiation. Human keratinocytes cultured on collagen rafts at the air-liquid interface produced a well-differentiated epithelium that resembled normal epidermis. Keratin filaments, membrane-coating granules, and keratohyalin granules were all observed. Epidermal differentiation markers keratin K1/K10, involucrin, and transglutaminase were localized in most of the suprabasal layers, whereas profilaggrin/filaggrin was confined to the granular layers and stratum corneum. In the continual presence of 10-20 ng/mL EGF, the epidermis was less organized, thinner, and less proliferative. EGF also depressed several indicators of differentiation: The number of keratohyalin granules and membrane-coating granules was greatly decreased; antigen expression of profilaggrin/filaggrin appeared diminished by immunocytochemical staining; frequent nuclear retention was noted in the relatively thickened stratum corneum-like layers. As detected by immunohistochemical staining, the expression of EGF receptor in the epidermis was reduced by exogenous EGF. These data illustrate that EGF cannot be considered a simple mitogen. Our findings also underscore the importance of using sophisticated culture models to assess complex cytokine effects that may be dependent on the architecture of a differentiating epidermis.

  14. A proteomic study showing differential regulation of stress, redox regulation and peroxidase proteins by iron supply and the transcription factor FER.

    Science.gov (United States)

    Brumbarova, Tzvetina; Matros, Andrea; Mock, Hans-Peter; Bauer, Petra

    2008-04-01

    Plants need to mobilize iron in the soil, and the basic helix-loop-helix transcription factor FER is a central regulator of iron acquisition in tomato roots. FER activity is controlled by iron supply. To analyse to what extent FER influences Fe-regulated protein expression, we investigated the root proteome of wild-type tomato, the fer mutant and a transgenic FER overexpression line under low-iron conditions versus sufficient and generous iron supply. The root proteomes were analysed by two-dimensional gel electrophoresis with three technical and three biological replicates. Statistical analysis identified 39 protein spots that were differentially regulated in selected pairwise comparisons of experimental conditions. Of these, 24 were correlated with expression clusters revealed by principal component analysis. The 39 protein spots were analysed by MALDI-TOF and nanoLC-MS/MS to deduce their possible functions. We investigated the functional representation in the identified expression clusters, and found that loss of FER function in iron-cultured plants mimicked an iron-deficiency status. The largest identified protein expression cluster was upregulated by iron deficiency and in the fer mutant. Two iron-regulated proteins required FER activity for induction by iron deficiency. Few proteins were suppressed by iron deficiency. The differentially expressed proteins belonged predominantly to the functional categories 'stress', 'redox regulation' and 'miscellaneous peroxidases'. Hence, we were able to identify distinct expression clusters of proteins with distinct functions.

  15. Protective factors in patients aged over 65 with stroke treated by physiotherapy, showing cognitive impairment, in the Valencia Community. Protection Study in Older People (EPACV

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    Gil-Guillen Vicente

    2012-10-01

    Full Text Available Abstract Background Family function may have an influence on the mental health deterioration of the caregivers of dependent family members and it could have a varying importance on the care of dependents. Little attention has been paid to the preparation of minor stroke survivors for the recovery trajectory or the spouse for the caregiving role. Therefore, this study protocol intends to analyze the influence of family function on the protection of patients with stroke sequels needing physiotherapy in the family environment. Methods/Design This is an analytical observational design, prospective cohort study and using a qualitative methodology by means of data collected in the “interviews of life”. The study will be carried out by the Rehabilitation Service at Hospital of Elda in the Valencia Community. All patients that have been diagnosed with stroke and need physiotherapy treatment, having a dependency grade assigned and consent to participate in the study, will undergo a monitoring of one year in order to assess the predictive factors depending on the dependence of the people affected. Discussion Our research aims to analyze the perception of caregivers, their difficulties to work, and the influence of family function. Moreover, it aims to register the perception of the patients with stroke sequel over the care received and whether they feel protected in their family environment.

  16. Treatment of rheumatoid arthritis with tumor necrosis factor inhibitors may predispose to significant increase in tuberculosis risk: a multicenter active-surveillance report.

    Science.gov (United States)

    Gómez-Reino, Juan J; Carmona, Loreto; Valverde, Vicente Rodríguez; Mola, Emilio Martín; Montero, Maria Dolores

    2003-08-01

    The long-term safety of therapeutic agents that neutralize tumor necrosis factor (TNF) is uncertain. Recent evidence based on spontaneous reporting shows an association with active tuberculosis (TB). We undertook this study to determine and describe the long-term safety of 2 of these agents, infliximab and etanercept, in rheumatic diseases based on a national active-surveillance system following the commercialization of the drugs. We analyzed the safety data actively collected in the BIOBADASER (Base de Datos de Productos Biológicos de la Sociedad Española de Reumatología) database, which was launched in February 2000 by the Spanish Society of Rheumatology. For the estimation of TB risk, the annual incidence rate in patients treated with these agents was compared with the background rate and with the rate in a cohort of patients with rheumatoid arthritis (RA) assembled before the era of anti-TNF treatment. Seventy-one participating centers sent data on 1,578 treatments with infliximab (86%) or etanercept (14%) in 1,540 patients. Drug survival rates (reported as the cumulative percentage of patients still receiving medication) for infliximab and etanercept pooled together were 85% and 81% at 1 year and 2 years, respectively. Instances of discontinuation were essentially due to adverse events. Seventeen cases of TB were found in patients treated with infliximab. The estimated incidence of TB associated with infliximab in RA patients was 1,893 per 100,000 in the year 2000 and 1,113 per 100,000 in the year 2001. These findings represent a significant increased risk compared with background rates. In the first 5 months of 2002, after official guidelines were established for TB prevention in patients treated with biologics, only 1 new TB case was registered (in January). Therapy with infliximab is associated with an increased risk of active TB. Proper measures are needed to prevent and manage this adverse event.

  17. The first double-blind, randomised, parallel-group certolizumab pegol study in methotrexate-naive early rheumatoid arthritis patients with poor prognostic factors, C-OPERA, shows inhibition of radiographic progression.

    Science.gov (United States)

    Atsumi, Tatsuya; Yamamoto, Kazuhiko; Takeuchi, Tsutomu; Yamanaka, Hisashi; Ishiguro, Naoki; Tanaka, Yoshiya; Eguchi, Katsumi; Watanabe, Akira; Origasa, Hideki; Yasuda, Shinsuke; Yamanishi, Yuji; Kita, Yasuhiko; Matsubara, Tsukasa; Iwamoto, Masahiro; Shoji, Toshiharu; Okada, Toshiyuki; van der Heijde, Désirée; Miyasaka, Nobuyuki; Koike, Takao

    2016-01-01

    To evaluate efficacy and safety of combination therapy using certolizumab pegol (CZP) and methotrexate (MTX) as first-line treatment for MTX-naive, early rheumatoid arthritis (RA) with poor prognostic factors, compared with MTX alone. MTX-naive, early RA patients with ≤12 months persistent disease, high anti-cyclic citrullinated peptide, and either rheumatoid factor positive and/or presence of bone erosions were enrolled in this multicentre, double-blind, randomised placebo (PBO)-controlled study. Patients were randomised 1:1 to CZP+MTX or PBO+MTX for 52 weeks. Primary endpoint was inhibition of radiographic progression (change from baseline in modified Total Sharp Score (mTSS CFB)) at week 52. Secondary endpoints were mTSS CFB at week 24, and clinical remission rates at weeks 24 and 52. 316 patients randomised to CZP+MTX (n=159) or PBO+MTX (n=157) had comparable baseline characteristics reflecting features of early RA (mean disease duration: 4.0 vs 4.3 months; Disease Activity Score 28-joint assessment (DAS28)) (erythrocyte sedimentation rate (ESR)): 5.4 vs 5.5; mTSS: 5.2 vs 6.0). CZP+MTX group showed significantly greater inhibition of radiographic progression relative to PBO+MTX at week 52 (mTSS CFB=0.36 vs 1.58; p<0.001) and week 24 (mTSS CFB=0.26 vs 0.86; p=0.003). Clinical remission rates (Simple Disease Activity Index, Boolean and DAS28 (ESR)) of the CZP+MTX group were significantly higher compared with those of the PBO+MTX group, at weeks 24 and 52. Safety results in both groups were similar, with no new safety signals observed with addition of CZP to MTX. In MTX-naive early RA patients with poor prognostic factors, CZP+MTX significantly inhibited structural damage and reduced RA signs and symptoms, demonstrating the efficacy of CZP in these patients. (NCT01451203). Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  18. Statistics Related Self-Efficacy A Confirmatory Factor Analysis Demonstrating a Significant Link to Prior Mathematics Experiences for Graduate Level Students

    Directory of Open Access Journals (Sweden)

    Karen Larwin

    2014-02-01

    Full Text Available The present study examined students' statistics-related self-efficacy, as measured with the current statistics self-efficacy (CSSE inventory developed by Finney and Schraw (2003. Structural equation modeling was used to check the confirmatory factor analysis of the one-dimensional factor of CSSE. Once confirmed, this factor was used to test whether a significant link to prior mathematics experiences exists. Additionally a new post-structural equation modeling (SEM application was employed to compute error-free latent variable score for CSSE in an effort to examine the ancillary effects of gender, age, ethnicity, department, degree level, hours completed, expected course grade, number of college-level math classes, current GPA on students' CSSE scores. Results support the one-dimensional construct and as expected, the model demonstrated a significant link between CSSE scores and prior mathematics experiences to CSSE. Additionally the students' department, expected grade, and number of prior math classes were found to have a significant effect on student's CSSE scores.

  19. A variant form of the human deleted in malignant brain tumor 1 (DMBT1) gene shows increased expression in inflammatory bowel diseases and interacts with dimeric trefoil factor 3 (TFF3).

    Science.gov (United States)

    Madsen, Jens; Sorensen, Grith Lykke; Nielsen, Ole; Tornøe, Ida; Thim, Lars; Fenger, Claus; Mollenhauer, Jan; Holmskov, Uffe

    2013-01-01

    The protein deleted in malignant brain tumors (DMBT1) and the trefoil factor (TFF) proteins have all been proposed to have roles in epithelial cell growth and cell differentiation and shown to be up regulated in inflammatory bowel diseases. A panel of monoclonal antibodies was raised against human DMBT1(gp340). Analysis of lung washings and colon tissue extracts by Western blotting in the unreduced state, two antibodies (Hyb213-1 and Hyb213-6) reacted with a double band of 290 kDa in lung lavage. Hyb213-6, in addition, reacted against a double band of 270 kDa in colon extract while Hyb213-1 showed no reaction. Hyb213-6 showed strong cytoplasmic staining in epithelial cells of both the small and large intestine whereas no staining was seen with Hyb213-1. The number of DMBT1(gp340) positive epithelial cells, stained with Hyb213-6, was significantly up regulated in inflammatory colon tissue sections from patients with ulcerative colitis (pTFF3 in a calcium dependent manner (pTFF3, TFF2 or glycosylated TFF2. This implies a role for DMBT1 and TFF3 together in inflammatory bowel disease.

  20. Significance of platelet activating factor receptor expression in pancreatic tissues of rats with severe acute pancreatitis and effects of BN52021

    Institute of Scientific and Technical Information of China (English)

    Shi-Hai Xia; Chun-Xiu Hu; Zhi-Ling Zhao; Guo-Dong Xia; Yao Di

    2007-01-01

    AIM: To investigate the dynamic changes and significance of platelet activating factor receptor (PAF-R)mRNA and protein in pancreatic tissues of rats with severe acute pancreatitis (SAP) and effects of BN52021(Ginkgolide B).METHODS: Wistar male rats were randomly assigned to the negative control group (NC group), SAP model group (SAP group), and BN52051-remedy group (BN group), and each of the groups was divided into 6 subgroups at different time points after operation (1 h,2 h, 3 h, 6 h, 12 h, and 24 h) (n = 10 in each). PT-PCR and Western blot methods were used to detect PAFRmRNA and protein expression in pancreatic tissues of rats respectively. Pathological examination of pancreatic tissues was performed and the serum amylase change was detected.RESULTS: Serum amylase and pathological results showed the that SAP model was successfully prepared,BN52021 was able to decrease serum amylase, and the pathological ratings in BN group at 3 h, 6 h, and 12 h significantly decreased compared with those in the SAP group (8.85 ± 0.39 vs 5.95 ± 0.19, 9.15 ± 0.55 vs 5.55 ± 0.36, 10.10 ± 0.65 vs 6.72 ± 0.30, P < 0.05). The result of PAF-mRNA showed dynamic changes in SAP and BN groups, which increased gradually in early stage,reached a peak at 3 h (0.71 ± 0.14 vs 0.54 ± 0.14,0.69 ± 0.13 vs 0.59 ± 0.04, P < 0.05), and decreased gradually later. There were significant differences at each time point except 1 h and 2 h, when compared with those in the NC group (0.71 ± 0.14 or 0.69 ± 0.13 vs 0.47 ± 0.10, 0.38 ± 0.08 or 0.59 ± 0.04 vs 0.47 ± 0.09, 0.25 ± 0.07 or 0.29 ± 0.05 vs 0.46 ± 0.10, 0.20 ± 0.06 or 0.20 ± 0.04 vs 0.43 ± 0.09, P < 0.05), whereas there was no significant difference between BN and SAP groups at each time point. The result of PAF-R protein showed that the change of PAF-R protein in the SAP group and the BN group was consistent with that of PAF-R mRNA.There were significant differences at each time point except 1 h, when compared with

  1. A variant form of the human deleted in malignant brain tumor 1 (DMBT1 gene shows increased expression in inflammatory bowel diseases and interacts with dimeric trefoil factor 3 (TFF3.

    Directory of Open Access Journals (Sweden)

    Jens Madsen

    Full Text Available The protein deleted in malignant brain tumors (DMBT1 and the trefoil factor (TFF proteins have all been proposed to have roles in epithelial cell growth and cell differentiation and shown to be up regulated in inflammatory bowel diseases. A panel of monoclonal antibodies was raised against human DMBT1(gp340. Analysis of lung washings and colon tissue extracts by Western blotting in the unreduced state, two antibodies (Hyb213-1 and Hyb213-6 reacted with a double band of 290 kDa in lung lavage. Hyb213-6, in addition, reacted against a double band of 270 kDa in colon extract while Hyb213-1 showed no reaction. Hyb213-6 showed strong cytoplasmic staining in epithelial cells of both the small and large intestine whereas no staining was seen with Hyb213-1. The number of DMBT1(gp340 positive epithelial cells, stained with Hyb213-6, was significantly up regulated in inflammatory colon tissue sections from patients with ulcerative colitis (p<0.0001 and Crohn's disease (p = 0.006 compared to normal colon tissue. Immunohistochemical analysis of trefoil factor TFF1, 2 and 3 showed that TFF1 and 3 localized to goblet cells in both normal colon tissue and in tissue from patients with ulcerative colitis or Crohn's disease. No staining for TFF2 was seen in goblet cells in normal colon tissue whereas the majority of tissue sections in ulcerative colitis and Crohn's disease showed sparse and scattered TFF2 positive goblet cells. DMBT1 and TFF proteins did therefore not co-localize in the same cells but localized in adjacent cells in the colon. The interaction between DMBT1(gp340 and trefoil TFFs proteins was investigated using an ELISA assay. DMBT1(gp340 bound to solid-phase bound recombinant dimeric TFF3 in a calcium dependent manner (p<0.0001 but did not bind to recombinant forms of monomeric TFF3, TFF2 or glycosylated TFF2. This implies a role for DMBT1 and TFF3 together in inflammatory bowel disease.

  2. Standardized uptake value in para-aortic lymph nodes is a significant prognostic factor in patients with primary advanced squamous cervical cancer

    Energy Technology Data Exchange (ETDEWEB)

    Yen, Tzu-Chen [Chang Gung Memorial Hospital Linkou Medical Center, Molecular Imaging Center and Department of Nuclear Medicine, Taoyuan (China); See, Lai-Chu [Chang Gung Memorial Hospital Linkou Medical Center, Biostatistics Consulting Center, Department of Public Health, Taoyuan (China); Lai, Chyong-Huey; Chao, Angel; Chang, Ting-Chang [Chang Gung Memorial Hospital Linkou Medical Center, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Taoyuan (China); Tsai, Chien-Sheng; Hong, Ji-Hong [Chang Gung Memorial Hospital Linkou Medical Center, Departments of Radiation Oncology, Taoyuan (China); Hsueh, Swei [Chang Gung Memorial Hospital Linkou Medical Center, Department of Pathology, Taoyuan (China); Ng, Koon-Kwan [Chang Gung Memorial Hospital Linkou Medical Center and Chang Gung University, Department of Medical Imaging and Intervention, Taoyuan (China)

    2008-03-15

    We sought to identify prognostic factors - including positron emission tomography (PET) parameters - in patients with previously untreated squamous carcinoma of the uterine cervix and MRI- or CT-defined pelvic or para-aortic lymph node (PLN or PALN) metastasis. Patients with untreated squamous cell cervical cancer and PLN or PALN metastasis detected by CT/MRI were enrolled. FDG-PET scans were performed for primary staging. Prognostic variables were investigated by univariate and multivariate analyses. Five-year recurrence-free and 5-year overall survivals (RFS and OS) were evaluated using the Kaplan-Meier method. A total of 70 patients [54 patients with International Federation of Gynecology and Obstetrics (FIGO) stage I or II, and 16 patients with stage III or IV] were eligible. Follow-up ranged from 26.1 to 71.6 months. In multivariate analysis, FIGO stage {>=}III (5-year RFS, p = 0.008; 5-year OS, p = 0.008) was a significant prognostic factor for both RFS and OS. In addition, SUV{sub max} for PALN (dichotomized by 3.3) was significantly associated with OS (p = 0.012) and marginally with RFS (p = 0.078). The presence of SUV{sub max} {>=} 3.3 at PALN or FIGO stage {>=}III were significantly associated with both recurrence [5-year RFS; HR = 4.52, 95% confidence interval (CI) = 1.73-11.80] and death (5-year OS; HR = 6.04, 95% CI = 1.97-18.57). SUV{sub max} {>=} 3.3 for PALN and FIGO stage {>=}III were significant adverse factors in patients with primary squamous cervical carcinoma and PLN or PALN metastasis detected by CT/MRI. (orig.)

  3. Detection and significance of serum insulin-like growth factor-1 in patients with type 2 diabetes, osteoporosis and type 2 diabetic osteoporosis

    Institute of Scientific and Technical Information of China (English)

    Yan-Rong Kang; Pei-Li Gu

    2016-01-01

    Objective:To investigate the content of insulin-like growth factor-1 (IGF-1) in serum and the relationship with type 2 diabetes, osteoporosis and type 2 diabetic osteoporosis.Methods:A total of 86 cases of patients with type 2 diabetes, 82 cases of patients with osteoporosis, 79 cases of patients with type 2 diabetic osteoporosis and 86 cases of healthy person were selected, the levels of IGF-1, diabetes related factors (fasting plasma c-peptide, FIN, HbA1c, GLU) and osteoporosis related factors (BMP, osteocalcin,β-CTx, P1NP, lumbar vertebra BMD) were detected, the relationship between the above indicators were compared with those of the disease.Results: In each group, content change of IGF-1 was not statistically significant; content changes of IGF-1, BMP and osteocalcin were control group>type 2 diabetes group>osteoporosis group>type 2 diabetic osteoporosis group. Diabetic osteoporosis enhanced the decrease of IGF-1 content. The contents ofβ-CTx and P1NP in osteoporosis group and diabetic osteoporosis group were similar, which were significantly lower than that in control group and type 2 diabetes group. The level of lumbar vertebra BMD in osteoporosis group and diabetic osteoporosis group were the lowest. Fasting plasma c-peptide in diabetes group and diabetic osteoporosis group were significantly lower than that in control group and osteoporosis group, and the content of fasting plasma c-peptide in diabetic osteoporosis group was the lowest. The contents of FIN, HbA1c and GLU in type 2 diabetes group and type 2 diabetic osteoporosis group were significantly higher than that in control group and osteoporosis group.Conclusion:IGF-1 was related with type 2 diabetes, osteoporosis and type 2 diabetic osteoporosis, and could offer help for predicting type 2 diabetes and osteoporosis in the future.

  4. Moderate Weight Reduction in an Outpatient Obesity Intervention Program Significantly Reduces Insulin Resistance and Risk Factors for Cardiovascular Disease in Severely Obese Adolescents

    Directory of Open Access Journals (Sweden)

    J. Grulich-Henn

    2011-01-01

    Full Text Available Background. Metabolic risk factors like insulin resistance and dyslipidemia are frequently observed in severly obese children. We investigated the hypothesis that moderate weight reduction by a low-threshold intervention is already able to reduce insulin resistance and cardiovascular risk factors in severely obese children. Methods. A group of 58 severely obese children and adolescents between 8 and 17 years participating in a six-month-long outpatient program was studied before and after treatment. The program included behavioral treatment, dietary education and specific physical training. Metabolic parameters were measured in the fasting state, insulin resistance was evaluated in an oral glucose tolerance test. Results. Mean standard deviation score of the body mass index (SDS-BMI in the study group dropped significantly from +2.5 ± 0.5 to 2.3 ± 0.6 (P<0.0001 after participation in the program. A significant decrease was observed in HOMA (6.3 ± 4.2 versus 4.9 ± 2.4, P<0.03, and in peak insulin levels (232.7 ± 132.4 versus 179.2 ± 73.3 μU/mL, P<0.006. Significant reductions were also observed in mean levels of hemoglobin A1c, total cholesterol and LDL cholesterol. Conclusions. These data demonstrate that already moderate weight reduction is able to decrease insulin resistance and dyslipidemia in severely obese children and adolescents.

  5. Clinical significance of tumor necrosis factor-α inhibitors in the treatment of sciatica: a systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Yun Fu Wang

    Full Text Available Currently, no satisfactory treatment is available for sciatica caused by herniated discs and/or spinal stenosis. The objective of this study is to assess the value of tumor necrosis factor (TNF-α inhibitors in the treatment of sciatica.Without language restrictions, we searched PubMed, OVID, EMBASE, the Web of Science, the Clinical Trials Registers, the Cochrane Central Register of Controlled Trials and the China Academic Library and Information System. We then performed a systematic review and meta-analysis on the enrolled trials that met the inclusion criteria.Nine prospective randomized controlled trials (RCTs and two before-after controlled trials involving 531 patients met our inclusion criteria and were included in this study. Our systematic assessment and meta-analysis demonstrated that in terms of the natural course of the disease, compared with the control condition, TNF-α inhibitors neither significantly relieved lower back and leg pain (both p > 0.05 nor enhanced the proportion of patients who felt overall satisfaction (global perceived effect (satisfaction or were able to return to work (return to work (combined endpoint; p > 0.05 at the short-term, medium-term and long-term follow-ups. In addition, compared with the control condition, TNF-α inhibitors could reduce the risk ratio (RR of discectomy or radicular block (combined endpoint; RR = 0.51, 95% CI 0.26 to 1.00, p = 0.049 at medium-term follow-up, but did not decrease RR at the short-term (RR = 0.64, 95% CI 0.17 to 2.40, p = 0.508 and long-term follow-ups (RR = 0.64, 95% CI 0.40 to 1.03, p = 0.065.The currently available evidence demonstrated that other than reducing the RR of discectomy or radicular block (combined endpoint at medium-term follow-up, TNF-α inhibitors showed limited clinical value in the treatment of sciatica caused by herniated discs and/or spinal stenosis.

  6. Low bone mineral density is a significant risk factor for low-energy distal radius fractures in middle-aged and elderly men: A case-control study

    Directory of Open Access Journals (Sweden)

    Johnsen Villy

    2011-04-01

    Full Text Available Abstract Background In general there is a lack of data on osteoporosis and fracture in men; this also includes low-energy distal radius fractures. The objectives of this study were to examine BMD and identify factors associated with distal radius fractures in male patients compared with controls recruited from the background population. Methods In a 2-year period, 44 men 50 years or older were diagnosed with low-energy distal radius fractures, all recruited from one hospital. The 31 men who attended for osteoporosis assessment were age-matched with 35 controls. Demographic and clinical data were collected and BMD at femoral neck, total hip and spine L2-4 was assessed by dual energy X-ray absorptiometry. Results Apart from weight and living alone, no significant differences were found between patient and controls for demographic variables (e.g. height, smoking and clinical variables (e.g. co-morbidity, use of glucocorticoids, osteoporosis treatment, falls and previous history of fracture. However, BMD expressed as T-score was significant lower in patients than in controls at all measurement sites (femoral neck: -2.24 vs. -1.15, p Conclusion The results from our study indicate that reduced BMD is an important risk factor for low-energy distal radius fracture in men. This suggests that improvement of BMD by both pharmacological and non-pharmacological initiatives may be a strategy to reduce fracture risk in men.

  7. Interactions outside the proteinase-binding loop contribute significantly to the inhibition of activated coagulation factor XII by its canonical inhibitor from corn.

    Science.gov (United States)

    Korneeva, Vera A; Trubetskov, Mikhail M; Korshunova, Alena V; Lushchekina, Sofya V; Kolyadko, Vladimir N; Sergienko, Olga V; Lunin, Vladimir G; Panteleev, Mikhail A; Ataullakhanov, Fazoil I

    2014-05-16

    Activated factor XII (FXIIa) is selectively inhibited by corn Hageman factor inhibitor (CHFI) among other plasma proteases. CHFI is considered a canonical serine protease inhibitor that interacts with FXIIa through its protease-binding loop. Here we examined whether the protease-binding loop alone is sufficient for the selective inhibition of serine proteases or whether other regions of a canonical inhibitor are involved. Six CHFI mutants lacking different N- and C-terminal portions were generated. CHFI-234, which lacks the first and fifth disulfide bonds and 11 and 19 amino acid residues at the N and C termini, respectively, exhibited no significant changes in FXIIa inhibition (Ki = 3.2 ± 0.4 nm). CHFI-123, which lacks 34 amino acid residues at the C terminus and the fourth and fifth disulfide bridges, inhibited FXIIa with a Ki of 116 ± 16 nm. To exclude interactions outside the FXIIa active site, a synthetic cyclic peptide was tested. The peptide contained residues 20-45 (Protein Data Bank code 1BEA), and a C29D substitution was included to avoid unwanted disulfide bond formation between unpaired cysteines. Surprisingly, the isolated protease-binding loop failed to inhibit FXIIa but retained partial inhibition of trypsin (Ki = 11.7 ± 1.2 μm) and activated factor XI (Ki = 94 ± 11 μm). Full-length CHFI inhibited trypsin with a Ki of 1.3 ± 0.2 nm and activated factor XI with a Ki of 5.4 ± 0.2 μm. Our results suggest that the protease-binding loop is not sufficient for the interaction between FXIIa and CHFI; other regions of the inhibitor also contribute to specific inhibition.

  8. Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold

    Directory of Open Access Journals (Sweden)

    RS Nirmal

    2013-11-01

    Full Text Available Stem cell based tissue engineering has emerged as a promising strategy for articular cartilage regeneration. Foetal derived mesenchymal stem cells (MSCs with their ease of availability, pluripotency and high expansion potential have been demonstrated to be an attractive cell source over adult MSCs. However, there is a need for optimisation of chondrogenic signals to direct the differentiation of these multipotent MSCs to chondrogenic lineage. In this study we have demonstrated the in vitro chondrogenesis of human umbilical cord matrix MSCs in three dimensional PVA-PCL (polyvinyl alcohol-polycaprolactone scaffolds in the presence of the individual growth factors TGFβ1, TGFβ3, IGF, BMP2 and their combination with BMP2. Gene expression, histology and immunohistology were evaluated after 28 d culture. The induced cells showed the feature of chondrocytes in their morphology and expression of typical chondrogenic extracellular matrix molecules. Moreover, the real-time PCR assay has shown the expression of gene markers of chondrogenesis, SOX9, collagen type II and aggrecan. The expression of collagen type I and collagen type X was also evaluated. This study has demonstrated the successful chondrogenic induction of human umbilical cord MSCs in 3D scaffolds. Interestingly, the growth factor combination of TGF-β3 and BMP-2 was found to be more effective for chondrogenesis as shown by the real-time PCR studies. The findings of this study suggest the importance of using growth factor combinations for successful chondrogenic differentiation of umbilical cord MSCs.

  9. Presence of a cerebral factor showing summer-morph-producing hormone activity in the brain of the seasonal non-polyphenic butterflies Vanessa cardui, V. indica and Nymphalis xanthomelas japonica (Lepidoptera: Nymphalidae)

    Institute of Scientific and Technical Information of China (English)

    Ayuki Tanaka; Moeko Inoue; Katsuhiko Endo; Chisato Kitazawa; Akira Yamanaka

    2009-01-01

    Three speciesofnymphalidbutterflies, Vanessacardui, V. indica and Nymphalis xanthomelas japonica, do not exhibit seasonal polyphenism in wing coloration. To deter-mine whether seasonal non-polyphenic butterflies possess a cerebral factor affecting wing coloration, we used a Polygonia c-aureum female short-day pupal assay for detection of summer-morph-producing hormone (SMPH) activity in P. c-aureum. When 2% NaCl extracts of 25 brain-equivalents prepared from the pupal brains of V. cardui, V. indica or N. xanthomelas japonica were injected into Polygonia female short-day pupae, all recipients developed into summer-morph adults with dark-yellow wings, and the average grade score (AGS) of summer morphs showing SMPH activity was 3.8, 3.7 and 4.0, respectively. In contrast, when acetone or 80% ethanol extracts prepared from pupal brains were injected into Polygonia pupae, all recipients developed into autumn-morph adults with a dark-brown coloration and each exhibited an AGS of less than 0.5. Our results indicate that a cerebral factor showing SMPH activity is present in the pupal brain of seasonal non-polyphenic nymphalid butterflies, suggesting that a SMPH and cerebral factor showing SMPH activity occur widely among butterfly species. This finding will improve our understanding of the presence of cerebral factors showing interspecific actions of SHPH.

  10. [The significance of social factors in choice of legal abortion. A social-medical study of 531 women applying for abortion and 285 pregnant control patients].

    Science.gov (United States)

    Wohlert, M; Larsen, F M

    1978-07-24

    Sociomedical factors were compared for a group for 531 women seeking abortion and 285 pregnant women, in order to give a differentiated picture of the social conditions which may motivate a woman to seek abortion and the stress which bearing a child can cause. Married women tended to want to carry the pregnancy to term in all age groups. Among the abortion seekers there was a significant overrepresentation of unskilled workers and students. The abortion frequency increased with the number of children. Each woman was classified according to whether her situation was characterized by a chronic social stress, an imminent social stress, or no social stress. The group designated by imminent social stress was significantly predominated by single women, the other two by married women. This group also had a significantly higher representation of students and unskilled workers. (p .0005) The chronic social stress group more often lived in apartments and had more children living at home. Among the abortion seekers, those with chronic situations most often gave as a reason for applying for abortion that they had enough children, that they were alone or had marital problems. Among the imminent stress group the reasons most often given were that the woman was too young or still pursuing her education. Among the group with no social stress, the most frequent reasons for seeking abortion were that the woman was too old or had enough children. It was concluded that bearing a child would cause actual social stress among ca. 50% of the women studied.

  11. Hypoxia-inducible factor-1α and semaphorin4D genes involved with tumor-associated macrophage-induced metastatic behavior and clinical significance in colon cancer.

    Science.gov (United States)

    Mu, Linjun; Wang, Jinshen; Chen, Yuezhi; Li, Leping; Guo, Xiaobo; Zheng, Sheng; Jing, Changqing

    2014-01-01

    Hypoxia promotes tumor angiogenesis and hypoxia-inducible factor-1 alpha (HIF-1α) plays a pivotal role in this process. Recently identified pro-angiogenic factor, semaphorin4D (Sema4D) also promotes angiogenesis and enhances invasive proliferation in some tumors. Furthermore, tumor-associated macrophages (TAMs) can increase the expression of HIF-1α and Sema4D in cancer cells and thus influence tumor growth and progression. The purpose of this study was to evaluate the effect of TAMs on the expression of Sema4D and HIF-1α and the impact of biologic behavior in colon cancer cells. Immunohistochemistry was used to analyze HIF-1α and Sema4D expression in 86 curatively resected colon cancer samples and 52 normal colon tissues samples. The relationship between their expression and clinicopathological factors was analyzed. Furthermore, macrophage-tumor cell interactions, such as metastasis, angiogenesis, were also studied using in vitro co-culture systems. Statistical analysis was performed using SPSS 17.0 software (SPSS Inc., USA). Differences between two groups were analyzed with Student's t test. HIF-1α (58%) and Sema4D (60%) were expressed at a significantly higher level in tumors than in normal tissues (P TNM stages (P 0.05). Sema4D expression was correlated with that of HIF-1α (r = 0.567, P colon cancer cells and subsequently increased their migration and invasion. HIF-1α and Sema4D expression are closely related to lymphatic metastasis, specific histological types and TNM stages in colon cancer. Furthermore, TAMs promote migration and invasion of colon cancer cells and endothelial tube formation, possibly through up-regulation of HIF-1α and Sema4D.

  12. Expression of vascular endothelial growth factor (VEGF) under hypoxia in placenta with intrahepatic cholestasis of pregnancy and its clinically pathological significance.

    Science.gov (United States)

    Zhang, Rong; Pan, Xiao-Hong; Xiao, Li

    2015-01-01

    The expression of vascular endothelial growth factor (VEGF) under hypoxia in the placenta with intrahepatic cholestasis of pregnancy (ICP) was observed, and mechanisms of ICP fetal distress were discussed. Different culturing times were established in hypoxia incubator, and protein expressions of VEGF in placental tissue were observed using immunohistochemical S-P method. After 4 h hypoxic culture, VEGF protein expression in ICP group was higher than the normal group with significant difference (P < 0.05). With the extension of hypoxic exposure, VEGF protein expression in both groups was suppressed, but no distinction in-between. Regression analyses indicated a noticeable effect of CG on VEGF expression, the higher the CG was, the lower the VEGF protein expression was (P < 0.05). Short term hypoxia induces up-regulation of VEGF expression in ICP placenta, and this adaptive change is probably a protective mechanism of fetus in ICP.

  13. Phosphorus, nitrogen and chlorophyll-a are significant factors controlling ciliate communities in summer in the northern Beibu Gulf, South China Sea.

    Directory of Open Access Journals (Sweden)

    Yibo Wang

    Full Text Available Ciliates (protozoa are ubiquitous components of plankton community and play important roles in aquatic ecosystems in regards of their abundance, biomass, diversity and energy turnover. Based on the stratified samples collected from the northern Beibu Gulf in August 2011, species composition, abundance, biomass, diversity and spatial pattern of planktonic ciliates were studied. Furthermore the main environmental factors controlling ciliate communities were determined. A total of 101 species belonging to 44 genera and 7 orders (i.e., Oligotrichida, Haptorida, Euplotida, Sessilida, Pleurostomatida, Scuticociliatida and Tintinnida were identified. The variation of ciliate communities was significant at horizontal level, but that was not at vertical level. Based on cluster analysis, ciliate communities were divided into three main groups. Redundancy analysis (RDA revealed that Group A, existing in the waters with higher concentration of phosphorus and nitrogen, was dominated by Tintinnidium primitivum. Group B in the waters with lower temperature and chlorophyll-a concentration, was dominated by Leegaardiella ovalis. Group C, existing in the waters with higher temperature and chlorophyll-a concentration, was dominated by large Strombidium spp. and Mesodinium rubrum. Combining multiple analytic methods, our results strongly supported that phosphorus, nitrogen and chlorophyll-a were the most significant factors affecting the ciliate communities in the northern Beibu Gulf in summer. Concentration of phosphorus and nitrogen primarily influenced ciliate biomass, implying a potential impact of eutrophication on ciliate growth. The correlation with chlorophyll-a concentration, on one hand indicate the response of ciliates to the food availability, and on the other hand, the ciliates containing chloroplasts or endosymbionts may contribute greatly to the chlorophyll-a.

  14. The expression of hypoxia-inducible factor-1α and its clinical significance in lung cancer: a systematic review and meta-analysis.

    Science.gov (United States)

    Ren, Weiwei; Mi, Denghai; Yang, Kehu; Cao, Nong; Tian, Jinhui; Li, Zheng; Ma, Bin

    2013-09-06

    Hypoxia-inducible factor-1α (HIF-1α) plays an important role in tumour progression and metastasis through activation of many target genes that are especially involved in pivotal aspects of cancer biology. However, the prognostic role of HIF-1α has been controversial in primary patients with lung cancer. This meta-analysis was performed to systematically evaluate whether HIF-1α expression is associated with the clinical outcomes in lung cancer patients. We retrieved relevant articles from Cochrane library, PubMed, EMbase, CNKI, CBM, VIP and Wan Fang Databases from inception to May 2012. Studies were selected using specific inclusion and exclusion criteria. A systematic review and meta-analysis was performed on the association between HIF-1α expression and clinical outcomes in lung cancer patients. All analyses were performed using the Revman 5.1 software. A total of 30 studies were identified as eligible for the systematic review and meta-analysis. The expression of HIF-1α was significantly higher than those in normal lung tissue; and III-IV stage, lymph node metastasis, poorly differentiation, squamous cell carcinoma and small cell lung cancer (SCLC) were significantly higher than those in I-II stage, no lymph node metastasis, well differentiation, adenocarcinomas and non small cell lung cancer (NSCLC), respectively (odds ratio (OR) = 19.00, 95% confidence interval (CI):12.12-29.78, p combines other proteins, such as vascular endothelial growth factor (VEGF) or CA IX, might serve as important parameters in evaluating biological behaviour and prognosis of lung cancer; it will be of benefit to clinical treatment and prognostic evaluation.

  15. Phosphorus, nitrogen and chlorophyll-a are significant factors controlling ciliate communities in summer in the northern Beibu Gulf, South China Sea.

    Science.gov (United States)

    Wang, Yibo; Zhang, Wenjing; Lin, Yuanshao; Cao, Wenqing; Zheng, Lianming; Yang, Jun

    2014-01-01

    Ciliates (protozoa) are ubiquitous components of plankton community and play important roles in aquatic ecosystems in regards of their abundance, biomass, diversity and energy turnover. Based on the stratified samples collected from the northern Beibu Gulf in August 2011, species composition, abundance, biomass, diversity and spatial pattern of planktonic ciliates were studied. Furthermore the main environmental factors controlling ciliate communities were determined. A total of 101 species belonging to 44 genera and 7 orders (i.e., Oligotrichida, Haptorida, Euplotida, Sessilida, Pleurostomatida, Scuticociliatida and Tintinnida) were identified. The variation of ciliate communities was significant at horizontal level, but that was not at vertical level. Based on cluster analysis, ciliate communities were divided into three main groups. Redundancy analysis (RDA) revealed that Group A, existing in the waters with higher concentration of phosphorus and nitrogen, was dominated by Tintinnidium primitivum. Group B in the waters with lower temperature and chlorophyll-a concentration, was dominated by Leegaardiella ovalis. Group C, existing in the waters with higher temperature and chlorophyll-a concentration, was dominated by large Strombidium spp. and Mesodinium rubrum. Combining multiple analytic methods, our results strongly supported that phosphorus, nitrogen and chlorophyll-a were the most significant factors affecting the ciliate communities in the northern Beibu Gulf in summer. Concentration of phosphorus and nitrogen primarily influenced ciliate biomass, implying a potential impact of eutrophication on ciliate growth. The correlation with chlorophyll-a concentration, on one hand indicate the response of ciliates to the food availability, and on the other hand, the ciliates containing chloroplasts or endosymbionts may contribute greatly to the chlorophyll-a.

  16. Please Show Yourself

    Institute of Scientific and Technical Information of China (English)

    刘艳丽; 孙俐

    2002-01-01

    We have experienced the social life since we were born. In the past days I have met a lot of people and affairs All those made me realize that there are masses of laughters and tears in our daily life Confidence, hardworking and determination will be the most three important factors if you like to get a shining future.

  17. KPC-producing Klebsiella pneumoniae enteric colonization acquired during intensive care unit stay: the significance of risk factors for its development and its impact on mortality.

    Science.gov (United States)

    Papadimitriou-Olivgeris, Matthaios; Marangos, Markos; Fligou, Fotini; Christofidou, Myrto; Sklavou, Christina; Vamvakopoulou, Sophia; Anastassiou, Evangelos D; Filos, Kriton S

    2013-10-01

    A prospective observational study of 226 intensive care unit (ICU) patients was conducted during a 25-month period. Rectal samples were taken at day 1, 4, and 7 and, afterwards, once weekly. Klebsiella pneumoniae was identified using standard techniques, whereas the presence of bla(KPC) gene was confirmed by PCR. During ICU stay, 72.6% of the patients were colonized with Klebsiella pneumoniae carbapenemases (KPC)-producing K. pneumoniae (KPC-Kp). Male gender, prior bed occupants, and patients in nearby beds colonized with KPC-Kp, tracheotomy, number of invasive catheters inserted, and number of antibiotics administered were the major risk factors for KPC-Kp colonization. ICU mortality (35.4%) was significantly related to Simplified Acute Physiology II score and respiratory insufficiency upon admission, cortisone administration, aminoglycoside administration, confirmed KPC-Kp infection, and severe sepsis or septic shock. The high prevalence of KPC-Kp enteric carriage in ICU patients and the significant mortality associated with KPC-Kp infection dictate the importance of early identification and isolation of such carriers.

  18. Prognostic Significance of Hypoxia-Inducible Factor Expression in Renal Cell Carcinoma: A PRISMA-compliant Systematic Review and Meta-Analysis.

    Science.gov (United States)

    Fan, Yang; Li, Hongzhao; Ma, Xin; Gao, Yu; Chen, Luyao; Li, Xintao; Bao, Xu; Du, Qingshan; Zhang, Yu; Zhang, Xu

    2015-09-01

    The prognostic value of hypoxia-inducible factor (HIF) in renal cell carcinoma (RCC) has been evaluated in a large number of studies, but the reports were inconsistent and remained inconclusive. Therefore, we conducted a systematic review and meta-analysis to clarify the significance of HIF expression in RCC prognosis. PubMed, Embase, Web of Science, Cochrane Library, EBSCO, Cumulative Index to Nursing and Allied Health Literature (CINAHL), and Biological Abstracts were searched for eligible studies. Hazard ratio (HR) data for overall survival (OS), cancer-specific survival (CSS), and progression-free survival (PFS) with 95% confidence interval (CI) related to the expression status of HIF-1α or HIF-2α detected by immunohistochemistry were all extracted. Data were combined using a random- or fixed-effects model based on the corresponding inter-study heterogeneity. Subgroup analyses were also performed. A total of 14 studies composed of 1258 patients for HIF-1α evaluation and 619 patients for HIF-2α evaluation were included for further analysis. When initially analyzed as a whole, the HIF-1α expression was not significantly correlated with OS (HR 1.637, 95% CI 0.898-2.985, P = 0.108), CSS (HR 1.110, 95% CI 0.595-2.069, P = 0.744), and PFS (HR 1.113, 95% CI 0.675-1.836, P = 0.674). Similarly, HIF-2α expression was not significantly correlated with CSS (HR 1.597, 95% CI 0.667-3.824, P = 0.293) and PFS (HR 0.847, 95% CI 0.566-1.266, P = 0.417). However, subgroup analyses concerning subcellular localization of HIFs revealed that the high nuclear expression of HIF-1α was significantly associated with poor OS (HR 2.014, 95% CI 1.206-3.363, P = 0.007) and the high cytoplasmic expression of HIF -2α was significantly associated with poor CSS (HR 2.356, 95% CI 1.629-3.407, P = 0.000). The increased nuclear expression of HIF-1α and cytoplasmic expression of HIF-2α indicate unfavorable prognosis in RCC patients, which may serve as biomarkers

  19. The expression of osteopontin and vascular endothelial growth factor in correlation with angiogenesis in monoclonal gammopathy of undetermined significance and multiple myeloma.

    Science.gov (United States)

    Babarović, Emina; Valković, Toni; Budisavljević, Ivana; Balen, Ivan; Štifter, Sanja; Duletić-Načinović, Antica; Lučin, Ksenija; Jonjić, Nives

    2016-06-01

    Several studies have shown a gradual increase in the extent of bone marrow angiogenesis in various stages of proliferative plasma cell disorders, from monoclonal gammopathy of undetermined significance (MGUS) to active multiple myeloma (MM). The main aim of this study was to evaluate tumor angiogenesis parameters in detail and to correlate them with the expression of osteopontin (OPN) and vascular endothelial growth factor (VEGF) in the bone marrow of patients with MGUS and MM. In addition, we wanted to determine their prognostic significance in active MM. Ninety-five patients were enrolled in the study: 14 diagnosed with MGUS, 13 with asymptomatic myeloma (AMM) and 68 with active MM. Computer assisted image analysis was used to determine the angiogenesis parameters, the quantity of microvessels per 1mm(2) (MVD), the area occupied by microvessels per 1mm(2) and the percentage of microvessel area in total section area (TVA). Double immunohistochemical methods CD138+VEGF and CD138+OPN were used to evaluate expression of these proteins in plasma cells, and OPN was also analyzed for its interstitial expression (iOPN). A significant positive correlation was determined between VEGF and iOPN with angiogenic parameters in the MGUS stage of the disease. In advanced stages of the disease, a significant negative correlation was recorded between OPN and iOPN with parameters of angiogenesis. Overall survival was significantly shorter for patients with negative iOPN (p=0.002) and higher angiogenic parameters, MVD (p=0.009), TVA (p=0.008) and area of microvessels per 1mm(2) (p=0.02). Positive VEGF expression in our model predicted a better three-year survival of patients with active MM (OR: 5.25, p=0.03; HR: 0.44, p=0.04). The results of our study suggested a possible key role of VEGF and OPN in the induction of angiogenesis in early-stage disease. Copyright © 2016 Elsevier GmbH. All rights reserved.

  20. Preoperative PET/CT standardized FDG uptake values of pelvic lymph nodes as a significant prognostic factor in patients with endometrial cancer

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Hyun Hoon; Kim, Hee Seung; Kim, Jae Weon; Park, Noh-Hyun [Seoul National University College of Medicine, Department of Obstetrics and Gynecology, Cancer Research Institute, 101 Daehak-ro, Jongno-gu, Seoul (Korea, Republic of); Cheon, Gi Jeong [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul (Korea, Republic of); Song, Yong Sang [Seoul National University College of Medicine, Department of Obstetrics and Gynecology, Cancer Research Institute, 101 Daehak-ro, Jongno-gu, Seoul (Korea, Republic of); Seoul National University, WCU Biomodulation Major, Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul (Korea, Republic of)

    2014-09-15

    Using integrated PET/CT, we evaluated the prognostic relevance of preoperative pelvic lymph node (LN) {sup 18}F-FDG uptake in endometrioid endometrial cancer. We retrospectively reviewed patients with pathologically proven endometrial cancer who underwent preoperative {sup 18}F-FDG PET/CT scans to evaluate the prognostic significance of PET/CT parameters and other clinicopathological variables. We used Cox proportional hazards regression to examine the relationship between recurrence and the maximum standardized uptake value (SUV{sub max}) in pelvic LNs (SUV{sub LN}) on FDG PET/CT. Clinical data, treatment modalities and results were reviewed in 70 eligible patients. The median postsurgical follow-up was 29 months (range 6 to 95 months). Receiver-operating characteristic analysis identified the significant SUV{sub LN} cut-off value as 15. The SUV{sub LN} correlated with FIGO stage (P < 0.001), LN metastasis (P < 0.001), lymphovascular space invasion (P < 0.001), SUV{sub tumour} (P = 0.001), metastatic LN size (P = 0.004), primary tumour size (P = 0.012), tumour grade (P = 0.015) and depth of tumour invasion (P = 0.035). Regression analysis showed a statistically significant association between recurrence and SUV{sub LN} (P = 0.002). Recurrence differed significantly (P < 0.001) between patients with SUV{sub LN} >15 and those with SUV{sub LN} ≤15. Preoperative pelvic LN FDG uptake exhibited a strong significant association with recurrence of endometrioid endometrial cancer. (orig.)

  1. The Health Show

    OpenAIRE

    Swann, David

    2011-01-01

    Dr David Swann interviewed on The Health Show, Series 1, Episode 5, 2011 for BBC World about the award-winning 21st Century Nursing Bag. BBC World News reaches 241million people every week, available in 296 million homes, 1.8 million hotel rooms and has the highest average viewership on a weekday of any international news channel. The Health Show is a new 26-part series for BBC World News covering the most important news stories from around the world.

  2. Lack of significant skin inflammation during elimination by apoptosis of large numbers of mouse cutaneous mast cells after cessation of treatment with stem cell factor.

    Science.gov (United States)

    Maurer, Marcus; Galli, Stephen J

    2004-12-01

    We previously reported that subcutaneous (s.c.) administration of stem cell factor (SCF), the ligand for the c-Kit receptor, to the back skin of mice promotes marked local increases in the numbers of cutaneous mast cells (MCs), and that cessation of SCF treatment results in the rapid reduction of cutaneous MC populations by apoptosis. In the present study, we used the 125I-fibrin deposition assay, a very sensitive method for quantifying increased vascular permeability, to assess whether the clearance of large numbers of apoptotic MCs is associated with significant cutaneous inflammation. The s.c. injection of rrSCF164 (30 or 100 microg/kg/day) or rrSCF164-peg (polyethylene glycol-treated SCF, 30 or 100 microg/kg/day) for 23 days increased the numbers of dermal MCs at skin injection sites from 5.1+/-0.7 MCs/mm2 to 36.4+/-4.1, 34.7+/-9.7, 52.5+/-5.8, and 545+/-97 MCs/mm2, respectively. In contrast, MC numbers were markedly lower in mice that had been treated with SCF for 21 days, followed by 2 days of injection with the vehicle alone. Notably, when tested during the period of rapid reduction of skin MCs,125I-fibrin deposition in the skin was very similar to that in mice receiving continuous treatment with SCF or vehicle. We conclude that the rapid elimination of even very large populations of MCs by apoptosis, which also results in the clearance of the considerable quantities of proinflammatory products stored by these cells, does not lead to significant local cutaneous inflammatory responses.

  3. Systemically administered interleukin-10 reduces tumor necrosis factor-alpha production and significantly improves functional recovery following traumatic spinal cord injury in rats.

    Science.gov (United States)

    Bethea, J R; Nagashima, H; Acosta, M C; Briceno, C; Gomez, F; Marcillo, A E; Loor, K; Green, J; Dietrich, W D

    1999-10-01

    In these studies, we examined the neuroprotective effects of the potent antiinflammatory cytokine interleukin-10 (IL-10) following spinal cord injury (SCI). Neuroprotection was assessed by using behavioral and morphological end points. We hypothesized that injury-induced inflammation contributes to the resulting neuropathology and subsequent loss of function. Therefore, by attenuating injury-induced inflammation, we should promote functional recovery. The New York University device was used to induce moderate SCI and study the resulting inflammatory response and functional consequences of inhibiting this response in rats. We determined that SCI induces the expression of tumor necrosis factor-alpha (TNF-alpha) in the spinal cord and by SCI-activated monocytes isolated from the peripheral circulation. IL-10 (5.0 microg) administered 30 minutes after-injury significantly reduced the expression of TNF-alpha protein in the spinal cord and in vitro by SCI-activated monocytes. Next, we investigated whether IL-10 would improve functional recovery after SCI. Randomized, double-blinded studies demonstrated that a single injection of IL-10 significantly improves hind limb motor function 2 months after injury, as determined by the Basso, Beattie and Bresnahan (BBB) open-field behavioral test. IL-10-treated animals had a mean BBB score of 18.0+/-0.5 (SEM, n = 9) compared with a score of 12.9+/-0.6 (SEM, n = 9) for the saline-treated controls. Morphological analysis demonstrated that IL-10 reduces lesion volume by approximately 49% 2 months after injury. These data suggest that acute administration of IL-10 reduces TNF-alpha synthesis in the spinal cord and by activated macrophages, is neuroprotective, and promotes functional recovery following SCI.

  4. Nab-paclitaxel Efficacy in the Orthotopic Model of Human Breast Cancer Is Significantly Enhanced By Concurrent Anti–Vascular Endothelial Growth Factor A Therapy

    Directory of Open Access Journals (Sweden)

    Lisa D. Volk

    2008-06-01

    Full Text Available Nab-paclitaxel is an albumin-bound 130-nm particle form of paclitaxel that has shown an improved efficacy in experimental tumor models and clinical studies compared with solvent-based paclitaxel. Anti–vascular endothelial growth factor A (VEGF-A antibody bevacizumab is known to enhance antitumor activity of cytotoxic drugs. This study evaluated the effects of combined nab-paclitaxel and bevacizumab therapy on growth and metastatic spread of orthotopic breast tumors. Cytotoxic and clonogenic assays measured VEGF-A–dependent modulation of nabpaclitaxel toxicity on cultured tumor cells. Antitumor effects were assessed in mice with luciferase-tagged, wellestablished MDA-MB-231 tumors (250–310 mm3 treated with one, two, or three cycles of nab-paclitaxel (10 mg/ kg, daily for five consecutive days, bevacizumab (2–8 mg/kg, twice a week, or with combination of both drugs. VEGF-A protected MDA-MB-231 cells against nab-paclitaxel cytotoxicity, whereas bevacizumab sensitized cells to the effect of the drug. Combined bevacizumab and nab-paclitaxel treatment synergistically inhibited tumor growth and metastasis resulting in up to 40% of complete regressions of well-established tumors. This therapy also decreased the incidence of lymphatic and pulmonary metastases by 60% and 100%, respectively. The significant increase in the cure of tumor-bearing mice in the nab-paclitaxel/bevacizumab combined group compared with mice treated with single drugs strongly advocates for implementing such strategy in clinics.

  5. Factors controlling reservoir quality in tertiary sandstones and their significance to geopressured geothermal production. Annual report, May 1, 1979-May 31, 1980

    Energy Technology Data Exchange (ETDEWEB)

    Loucks, R.G.; Richmann, D.L.; Milliken, K.L.

    1980-07-01

    Differing extents of diagenetic modification is the factor primarily responsible for contrasting regional reservoir quality of Tertiary sandstones from the Upper and Lower Texas Gulf Coast. Detailed comparison of Frio sandstones from the Chocolate Bayou/Danbury Dome area, Brazoria County, and Vicksburg sandstones from the McAllen Ranch Field area, Hidalgo County, reveals that extent of diagenetic modification is most strongly influenced by (1) detrital mineralogy and (2) regional geothermal gradients. Vicksburg sandstones from the McAllen Ranch Field area are less stable, chemically and mechanically, than Frio sandstones from the Chocolate Bayou/Danbury dome area. Vicksburg sandstones are mineralogically immature and contain greater proportions of feldspars and rock fragments than do Frio sandstones. Thr reactive detrital assemblage of Vicksubrg sandstones is highly susceptible to diagenetic modification. Susceptibility is enhanced by higher than normal geothermal gradients in the McAllen Ranch Field area. Thus, consolidation of Vicksburg sandstones began at shallower depth of burial and precipitation of authigenic phases (especially calcite) was more pervasive than in Frio sandstones. Moreover, the late-stage episode of ferroan calcite precipitation that occluded most secondary porosity in Vicksburg sandstones did not occur significantly in Frio sandstones. Therefore, regional reservoir quality of Frio sandstones from Brazoria County is far better than that characterizing Vicksburg sandstones from Hidalgo County, especially at depths suitable for geopressured geothermal energy production.

  6. A Fashion Show

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    <正>Story: The yearly fashion show day.The children take turns to walk on the stage and show the class their favorite clothes.Now it’s Joe’s and Phoebe’s turn.Joe walks on the stage and says,“My shorts are blue.Do you like my blue shorts?”On the other side of the stage, Phoebe is wearing her favorite pink skirt.“My skirt is pink.Do you like my pink skirt?”asks

  7. 转录因子Snail在乳腺癌中的表达及意义%Expression and Significance of Transcription Factor Snail in Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    赵典朋; 申安; 李世平; 刘桂玲; 尹崇高

    2011-01-01

    目的 检测转录因子Snail mRNA及蛋白在乳腺癌中的表达,探讨Snail在乳腺癌发生发展中的作用.方法 采用免疫组织化学、RT-PCR的方法检测乳腺癌、癌旁相对正常乳腺组织(>5 cm)中转录因子Snail mRNA及蛋白的表达,并分析其与临床病理参数之间的关系.结果 乳腺癌组织中Snail mRNA的阳性表达率高于癌旁相对正常乳腺组织(P<0.05);乳腺癌组织中Snail mRNA的阳性表达量高于癌旁相对正常乳腺组织(P<0.05).乳腺癌组织中Snail蛋白的阳性表达率高于癌旁相对正常乳腺组织(P<0.05),且癌组织中Snail蛋白阳性表达率与淋巴结转移有关(P<0.05),而与TNM分期、分化程度无关.结论 乳腺癌的癌变、侵袭、转移可能与Snail蛋白、mRNA的高表达有关.%Objective To detect the expression of transcription factor Snail mRNA in breast cancer tissues and its role in the development of breast cancer. Methods The expression of Snail protein and mRNA in breast cancer and normal breast tissue were detected by immunohistochemistry and RT-PCR. The relatives of Snail and clink-olhological parameters were analyzed. Results The positive rate of Snail mRNA were significantly higher in cancer tissues than that in normal breast tissues(P <0.05); The expression of Snail mRNA were significantly higher in cancer tissues than that in normal breast tissues (P <0.05). The positive rate of Snail protein were significantly higher in cancer tissues than that in normal breast tissues ( P < 0.05). The expression of Snail in cancer tissue were correlated with lymphnode metastasis(P <0.05) and were not correlated with TNM stage and differentiation grade. Conclusion The carcinogenesis, invasion and metastasis of breast cancer may be related to overexpression of Snail protein and mRNA.

  8. On not showing scalps

    DEFF Research Database (Denmark)

    Marselis, Randi Lorenz

    2016-01-01

    proposed by Janet Marstine, the editor of the Routledge Companion to Museum Ethics, I show how the museum succeeded in engaging users in questions of museum ethics. However, this specific debate on human remains in museums developed into an encounter between a global, museological discourse...

  9. Violence and TV Shows

    OpenAIRE

    ÖZTÜRK, Yrd. Doç. Dr. Şinasi

    2008-01-01

    This study aims to discuss theories on theviolent effects of TV shows on viewers, especiallyon children. Therefore, this study includes a briefdiscussion of definitions of violence, discussionof violence theories, main results of researcheson televised violence, measuring TV violence,perception of televised violence, individualdifferences and reactions to TV violence,aggressiveness and preferences for TV violence.

  10. Honored Teacher Shows Commitment.

    Science.gov (United States)

    Ratte, Kathy

    1987-01-01

    Part of the acceptance speech of the 1985 National Council for the Social Studies Teacher of the Year, this article describes the censorship experience of this honored social studies teacher. The incident involved the showing of a videotape version of the feature film entitled "The Seduction of Joe Tynan." (JDH)

  11. A Visionary Show

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Seduction. Distinction. Relax. Pulsation. These are the "style universes" on display at Première Vision, heralded as "The World’s Premiere Fabric Show." Started more than 35 years ago by 15 French weavers, Première Vision has expanded beyond its

  12. Honored Teacher Shows Commitment.

    Science.gov (United States)

    Ratte, Kathy

    1987-01-01

    Part of the acceptance speech of the 1985 National Council for the Social Studies Teacher of the Year, this article describes the censorship experience of this honored social studies teacher. The incident involved the showing of a videotape version of the feature film entitled "The Seduction of Joe Tynan." (JDH)

  13. Shanghai Shows Its Heart

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The city known as China’s economic powerhouse showed a more caring face as host of the Special Olympic Games Between October 2 and 11,the Special Olympics Summer Games were hosted in Shanghai,the first time the 40-year-old athletic com- petition for people with intellectual disabilities came to a developing country. This Special Olympics was also larger than all previous games in temps of the number of athletes.

  14. Stunting, poor iron status and parasite infection are significant risk factors for lower cognitive performance in Cambodian school-aged children.

    Directory of Open Access Journals (Sweden)

    Marlene Perignon

    Full Text Available BACKGROUND: Nutrition is one of many factors affecting the cognitive development of children. In Cambodia, 55% of children <5 y were anemic and 40% stunted in 2010. Currently, no data exists on the nutritional status of Cambodian school-aged children, or on how malnutrition potentially affects their cognitive development. OBJECTIVE: To assess the anthropometric and micronutrient status (iron, vitamin A, zinc, iodine of Cambodian schoolchildren and their associations with cognitive performance. METHODS: School children aged 6-16 y (n = 2443 from 20 primary schools in Cambodia were recruited. Anthropometry, hemoglobin, serum ferritin, transferrin receptors, retinol-binding protein and zinc concentrations, inflammation status, urinary iodine concentration and parasite infection were measured. Socio-economic data were collected in a sub-group of children (n = 616. Cognitive performance was assessed using Raven's Colored Progressive Matrices (RCPM and block design and picture completion, two standardized tests from the Wechsler Intelligence Scale for Children (WISC-III. RESULTS: The prevalence of anemia, iron, zinc, iodine and vitamin A deficiency were 15.7%; 51.2%, 92.8%, 17.3% and 0.7% respectively. The prevalence of stunting was 40.0%, including 10.9% of severe stunting. Stunted children scored significantly lower than non-stunted children on all tests. In RCPM test, boys with iron-deficiency anemia had lower scores than boys with normal iron status (-1.46, p<0.05. In picture completion test, children with normal iron status tended to score higher than iron-deficient children with anemia (-0.81; p = 0.067 or without anemia (-0.49; p = 0.064. Parasite infection was associated with an increase in risk of scoring below the median value in block design test (OR = 1.62; p<0.05, and with lower scores in other tests, for girls only (both p<0.05. CONCLUSION: Poor cognitive performance of Cambodian school-children was multifactorial and

  15. Is Hemoglobin Level in Patients with Nasopharyngeal Carcinoma Still a Significant Prognostic Factor in the Era of Intensity-Modulated Radiotherapy Technology?

    Science.gov (United States)

    Guo, Shan-Shan; Tang, Lin-Quan; Chen, Qiu-Yan; Zhang, Lu; Liu, Li-Ting; Huang, Pei-Yu; Cao, Ka-Jia; Guo, Ling; Mo, Hao-Yuan; Guo, Xiang; Hong, Ming-Huang; Zeng, Mu-Sheng; Qian, Chao-Nan; Mai, Hai-Qiang

    2015-01-01

    Hemoglobin (Hb) levels are regarded as an important determinant of outcome in a number of cancers treated with radiotherapy. However, for patients treated with intensity modulated radiotherapy (IMRT), information regarding the prognostic value of hemoglobin level is scarce. A total of 650 patients with nasopharyngeal carcinoma (NPC), enrolled between May, 2005, and November, 2012, were included in this study. The prognostic significance of hemoglobin level (anemia or no-anemia) at three different time points was investigated, including before treatment, during treatment and at the last week of treatment. Univariate and multivariate analyses were conducted using the log-rank test and the Cox proportional hazards model, respectively. The 5-year OS (overall survival) rate of patients who were anemia and no-anemia before treatment were 89.1%, and 80.7% (P = 0.01), respectively. The 5-year DMFS (distant metastasis-free survival) rate of patients who were anemia and no-anemia before treatment were 88.9%, and 78.2% (P = 0.01), respectively. The 5-year OS rate of patients who were anemia and no-anemia during treatment were 91.7% and 83.3% (P = 0.004). According to multivariate analysis, the pre-treatment Hb level predicted a decreased DMFS (P = 0.007, HR = 2.555, 95% CI1.294-5.046). Besides, the mid-treatment Hb level predicted a decreased OS (P = 0.013, HR = 2.333, 95% CI1.199-4.541). Hemoglobin level is a useful prognostic factor in NPC patients receiving IMRT. It is important to control the level of hemoglobin both before and during chemoradiotherapy.

  16. Not a "reality" show.

    Science.gov (United States)

    Wrong, Terence; Baumgart, Erica

    2013-01-01

    The authors of the preceding articles raise legitimate questions about patient and staff rights and the unintended consequences of allowing ABC News to film inside teaching hospitals. We explain why we regard their fears as baseless and not supported by what we heard from individuals portrayed in the filming, our decade-long experience making medical documentaries, and the full un-aired context of the scenes shown in the broadcast. The authors don't and can't know what conversations we had, what documents we reviewed, and what protections we put in place in each televised scene. Finally, we hope to correct several misleading examples cited by the authors as well as their offhand mischaracterization of our program as a "reality" show.

  17. Analysis of Anemia Factors and Significances in Crohn's Disease%克罗恩病患者贫血因素分析及其临床意义

    Institute of Scientific and Technical Information of China (English)

    刘嫦钦; 吴维; 邬瑞金; 陈腾飞; 翁韵; 赵玉洁; 夏玉敬; 汤茂春; 刘占举

    2013-01-01

    Background: The incidence of Crohn' s disease ( CD ) in China is increasing, and its accompanied disease such as anemia has received more attention. Aims: To investigate the anemia factors and their significances in CD patients. Methods: Forty CD patients from June 2011 to August 2012 at Shanghai Tenth People' s Hospital of Tongji University were enrolled. The levels of hemoglobin ( Hb ), serum iron, total iron binding capacity, folic acid, vitamin B12 between different subtypes of CD according to Montreal classification were compared, and the relationship of Crohn' s disease activity index ( CDAI )/CRP with Hb/serum iron was analyzed. Results: Of the 40 patients, anemia occurred in 34 patients ( 85.0% ). No significant differences in Hb, serum iron, total iron binding capacity, folic acid were found between patients with different subtypes of CD according to Montreal classification ( P = 0. 2061, P = 0. 2449, P = 0. 8007, P = 0. 8804 ), whereas significant difference was found in vitamin B12( P =0.0072 ). A negative correlation was found between CDAI/CRP and Hb/serum iron ( P < 0. 05 ). Conclusions: Patients with type LI and type L3 CD of Montreal classification have vitamin B12 -associated anemia, and the decrease of Hb and serum iron is correlated with the increase of inflammatory activity.%背景:我国克罗恩病(CD)的发病率日益增长,其伴随的疾病如贫血等正逐渐受到关注.目的:探讨CD患者贫血因素及其临床意义.方法:纳入2011年6月~2012年8月同济大学附属第十人民医院40例CD患者,比较不同蒙特利尔分型的患者之间血红蛋白(Hb)、血清铁、总铁结合力、叶酸、维生素B12水平,并分析克罗恩病活动指数(CDAI)、CRP水平与Hb、血清铁的相关性.结果:34例(85.0%)CD患者伴有贫血.不同蒙特利尔分型的CD患者之间Hb、血清铁、总铁结合力、叶酸水平无明显差异(P=0.2061,P=0.2449,P=0.8007,P=0.8804),而维生素B12水平差异有统计学意义(P=0

  18. Showing Value (Editorial

    Directory of Open Access Journals (Sweden)

    Denise Koufogiannakis

    2009-06-01

    Full Text Available When Su Cleyle and I first decided to start Evidence Based Library and Information Practice, one of the things we agreed upon immediately was that the journal be open access. We knew that a major obstacle to librarians using the research literature was that they did not have access to the research literature. Although Su and I are both academic librarians who can access a wide variety of library and information literature from our institutions, we belong to a profession where not everyone has equal access to the research in our field. Without such access to our own body of literature, how can we ever hope for practitioners to use research evidence in their decision making? It would have been contradictory to the principles of evidence based library and information practice to do otherwise.One of the specific groups we thought could use such an open access venue for discovering research literature was school librarians. School librarians are often isolated and lacking access to the research literature that may help them prove to stakeholders the importance of their libraries and their role within schools. Certainly, school libraries have been in decline and the use of evidence to show value is needed. As Ken Haycock noted in his 2003 report, The Crisis in Canada’s School Libraries: The Case for Reform and Reinvestment, “Across the country, teacher-librarians are losing their jobs or being reassigned. Collections are becoming depleted owing to budget cuts. Some principals believe that in the age of the Internet and the classroom workstation, the school library is an artifact” (9. Within this context, school librarians are looking to our research literature for evidence of the impact that school library programs have on learning outcomes and student success. They are integrating that evidence into their practice, and reflecting upon what can be improved locally. They are focusing on students and showing the impact of school libraries and

  19. Arabidopsis Plants Having Defects in Nonsense-mediated mRNA Decay Factors UPF1,UPF2, and UPF3 Show Photoperiod-dependent Phenotypes in Development and Stress Responses

    Institute of Scientific and Technical Information of China (English)

    Chuan Shi; lan T. Baldwin; Jianqiang Wu

    2012-01-01

    Nonsense-mediated mRNA decay (NMD) is an important mRNA quality surveillance pathway in all eukaryotes that eliminates aberrant mRNAs derived from various sources.Three NMD factor proteins,UPF1,UPF2,and UPF3 are required for the NMD process and were found to be also involved in certain stress responses in mammalian and yeast cells.Using Arabidopsis thaliana mutants of UPF1 and UPF3 and UPF2-silenced lines (irUPF2),we examined the involvement of UPF1,UPF2,and UPF3 in development and in response to stresses,wounding and infection by Pseudomonas syringae pv.tomato strain DC3000.Under the long (16 h) photoperiod condition,Arabidopsis having a defect in NMD factors exhibited altered morphologies of various organs,disturbed homeostasis of wounding-induced jasmonic acid and pathogen-elicited salicylic acid,and abnormal wounding- and methyl jasmonate-induced changes in the transcript levels of two defense-related genes,LOX2 and VSP2.Importantly,when plants were cultivated under the short (10 h) photoperiod condition,mutants of UPF1 and UPF3 and irUPF2 showed smaller differences from the wild-type plants in growth and stress-induced responses.These data suggest a complex regulatory network,likely composed of light signaling and NMD factor-mediated pathways,in influencing plant development and adaption to environmental stresses.

  20. Public medical shows.

    Science.gov (United States)

    Walusinski, Olivier

    2014-01-01

    In the second half of the 19th century, Jean-Martin Charcot (1825-1893) became famous for the quality of his teaching and his innovative neurological discoveries, bringing many French and foreign students to Paris. A hunger for recognition, together with progressive and anticlerical ideals, led Charcot to invite writers, journalists, and politicians to his lessons, during which he presented the results of his work on hysteria. These events became public performances, for which physicians and patients were transformed into actors. Major newspapers ran accounts of these consultations, more like theatrical shows in some respects. The resultant enthusiasm prompted other physicians in Paris and throughout France to try and imitate them. We will compare the form and substance of Charcot's lessons with those given by Jules-Bernard Luys (1828-1897), Victor Dumontpallier (1826-1899), Ambroise-Auguste Liébault (1823-1904), Hippolyte Bernheim (1840-1919), Joseph Grasset (1849-1918), and Albert Pitres (1848-1928). We will also note their impact on contemporary cinema and theatre.

  1. The Great Cometary Show

    Science.gov (United States)

    2007-01-01

    its high spatial and spectral resolution, it was possible to zoom into the very heart of this very massive star. In this innermost region, the observations are dominated by the extremely dense stellar wind that totally obscures the underlying central star. The AMBER observations show that this dense stellar wind is not spherically symmetric, but exhibits a clearly elongated structure. Overall, the AMBER observations confirm that the extremely high mass loss of Eta Carinae's massive central star is non-spherical and much stronger along the poles than in the equatorial plane. This is in agreement with theoretical models that predict such an enhanced polar mass-loss in the case of rapidly rotating stars. ESO PR Photo 06c/07 ESO PR Photo 06c/07 RS Ophiuchi in Outburst Several papers from this special feature focus on the later stages in a star's life. One looks at the binary system Gamma 2 Velorum, which contains the closest example of a star known as a Wolf-Rayet. A single AMBER observation allowed the astronomers to separate the spectra of the two components, offering new insights in the modeling of Wolf-Rayet stars, but made it also possible to measure the separation between the two stars. This led to a new determination of the distance of the system, showing that previous estimates were incorrect. The observations also revealed information on the region where the winds from the two stars collide. The famous binary system RS Ophiuchi, an example of a recurrent nova, was observed just 5 days after it was discovered to be in outburst on 12 February 2006, an event that has been expected for 21 years. AMBER was able to detect the extension of the expanding nova emission. These observations show a complex geometry and kinematics, far from the simple interpretation of a spherical fireball in extension. AMBER has detected a high velocity jet probably perpendicular to the orbital plane of the binary system, and allowed a precise and careful study of the wind and the shockwave

  2. Significance of pretreatment cardiovascular morbidity as a risk factor during treatment with parenteral oestrogen or combined androgen deprivation of 915 patients with metastasized prostate cancer

    DEFF Research Database (Denmark)

    Johansson, Robert; Damber, Jan Erik; Hagerman, Inger

    2011-01-01

    This study aimed to evaluate prognostic risk factors for cardiovascular events during treatment of metastatic prostate cancer patients with high-dose parenteral polyoestradiol phosphate (PEP, Estradurin®) or combined androgen deprivation (CAD) with special emphasis on pretreatment cardiovascular...

  3. C-reactive protein, established risk factors and social inequalities in cardiovascular disease – the significance of absolute versus relative measures of disease

    Directory of Open Access Journals (Sweden)

    Hedblad Bo

    2008-06-01

    Full Text Available Abstract Background The widespread use of relative scales in socioepidemiological studies has recently been criticized. The criticism is based mainly on the fact that the importance of different risk factors in explaining social inequalities in cardiovascular disease (CVD varies, depending on which scale is used to measure social inequalities. The present study examines the importance of established risk factors, as opposed to low-grade inflammation, in explaining socioeconomic differences in the incidence of CVD, using both relative and absolute scales. Methods We obtained information on socioeconomic position (SEP, established risk factors (smoking, hypertension, and hyperlipidemia, and low-grade inflammation as measured by high-sensitive (hs C-reactive protein (CRP levels, in 4,268 Swedish men and women who participated in the Malmö Diet and Cancer Study (MDCS. Data on first cardiovascular events, i.e., stroke or coronary event (CE, was collected from regional and national registers. Social inequalities were measured in relative terms, i.e., as ratios between incidence rates in groups with lower and higher SEP, and also in absolute terms, i.e., as the absolute difference in incidence rates in groups with lower and higher SEP. Results Those with low SEP had a higher risk of future CVD. Adjustment for risk factors resulted in a rather small reduction in the relative socioeconomic gradient, namely 8% for CRP (≥ 3 mg/L and 21% for established risk factors taken together. However, there was a reduction of 18% in the absolute socioeconomic gradient when looking at subjects with CRP-levels Conclusion C-reactive protein and established risk factors all contribute to socioeconomic differences in CVD. However, conclusions on the importance of "modern" risk factors (here, CRP, as opposed to established risk factors, in the association between SEP and CVD depend on the scale on which social inequalities are measured. The one-sided use of the relative

  4. Back-calculation method shows that within-flock transmission of highly pathogenic avian influenza (H7N7) virus in the Netherlands is not influenced by housing risk factors.

    Science.gov (United States)

    Bos, Marian E H; Nielen, Mirjam; Koch, Guus; Bouma, Annemarie; De Jong, Mart C M; Stegeman, Arjan

    2009-04-01

    To optimize control of an avian influenza outbreak knowledge of within-flock transmission is needed. This study used field data to estimate the transmission rate parameter (beta) and the influence of risk factors on within-flock transmission of highly pathogenic avian influenza (HPAI) H7N7 virus in the 2003 epidemic in The Netherlands. The estimation is based on back-calculation of daily mortality data to fit a susceptible-infectious-dead format, and these data were analysed with a generalized linear model. This back-calculation method took into account the uncertainty of the length of the latent period, the survival of an infection by some birds and the influence of farm characteristics. After analysing the fit of the different databases created by back-calculation, it could be concluded that an absence of the latency period provided the best fit. The transmission rate parameter (beta) from these field data was estimated at 4.50 per infectious chicken per day (95% CI: 2.68-7.57), which was lower than what was reported from experimental data. In contrast to general belief, none of the studied risk factors (housing system, flock size, species, age of the birds in weeks and date of depopulation) had significant influence on the estimated beta.

  5. Diabetes mellitus, metabolic syndrome and obesity are not significant risk factors for hepatocellular carcinoma in an HBV- and HCV-endemic area of Southern Taiwan

    Directory of Open Access Journals (Sweden)

    Chao-Tung Chen

    2013-08-01

    Full Text Available A prominent factor in hepatocellular carcinoma (HCC is chronic infection with hepatitis B virus (HBV and hepatitis C virus (HCV. Diabetes mellitus (DM, metabolic syndrome (MetS, and obesity have also been implicated in HCC development, but these associations are not observed in all HBV- and HCV-endemic areas. We attempted to clarify the role of these factors in HCC development in an HBV- and HCV-endemic area in southern Taiwan. A community-based health examination was conducted in 2004 in Tainan County. After individuals with incomplete data and those with known HCC were excluded, there were 56,231 participants who were over 40 years of age. A further 262 HCC cases were identified from the National Cancer Registration Database records from 2005 to 2007. The hepatitis B surface antigen (HBsAg seropositivity, anti-HCV seropositivity, platelet count, serum biochemical data, blood pressure, sociodemographic information, and anthropometric measurements were analyzed. Survival analyses were used to identify the associations between these factors and HCC. For the 262 HCC cases, male gender and age greater than 65 years were risk factors. Furthermore, a high alanine aminotransferase level, chronic HBV and/or HCV infection, and liver cirrhosis were also risk factors for HCC. However, DM, MetS and obesity were not associated with HCC development in the non-HBV-/non-HCV-infected, HBV, HCV, or dual B/C groups. In this HBV- and HCV- endemic area, DM, MetS and obesity were not risk factors for developing HCC.

  6. The PNPLA3 rs738409 148M/M genotype is a risk factor for liver cancer in alcoholic cirrhosis but shows no or weak association in hepatitis C cirrhosis.

    Directory of Open Access Journals (Sweden)

    Hans Dieter Nischalke

    Full Text Available BACKGROUND: An isoleucine>methionine mutation at position 148 in the PNPLA3 gene (p.I148M, rs738409 has recently been identified as a susceptibility factor for liver damage in steatohepatitis. Here, we studied whether the PNPLA3 rs738409 polymorphism also affects predisposition to hepatocellular carcinoma (HCC. METHODS: We compared distributions of PNPLA3 genotypes in 80 and 81 Caucasian patients with alcoholic and hepatitis C virus (HCV-associated HCC to 80 and 81 age- and sex-matched patients with alcohol-related and HCV-related cirrhosis without HCC, respectively. PNPLA3 genotypes in 190 healthy individuals from the same population served as reference. Potential confounders obesity, diabetes, HCV genotype and HBV co-infection were controlled by univariate and multivariate logistic regression with forward variable selection. RESULTS: PNPLA3 genotypes were in Hardy-Weinberg equilibrium for all study groups. The frequency of the 148M allele was significantly (p<0.001 increased in alcoholic cirrhosis with (53.7% and without HCC (36.2% but was not different between healthy controls (22.9% and patients with cirrhosis (25.3%; p = 0.545 and HCC (30.2%; p = 0.071 due to hepatitis C. HCC risk was highest in 148M/M homozygous patients with alcoholic liver disease (odds ratio (OR 16.8 versus healthy controls; 95% confidence interval (CI 6.68-42.43, p<0.001. Finally, multivariate regression confirmed 148M/M homozygosity (OR 2.8; 95%-CI: 1.24-6.42; p = 0.013 as HCC risk factor in alcoholic cirrhosis. In HCV-related cirrhosis only HCV genotype 1 was confirmed as a HCC risk factor (OR 4.2; 95%-CI: 1.50-11.52; p = 0.006. CONCLUSION: The PNPLA3 148M variant is a prominent risk factor for HCC in patients with alcoholic cirrhosis, while its effects are negligible in patients with cirrhosis due to HCV. This polymorphism provides an useful tool to identify individuals with particularly high HCC risk in patients with alcoholic liver disease that

  7. Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors

    DEFF Research Database (Denmark)

    Sorkin, A; Helin, K; Waters, C M

    1992-01-01

    The role of epidermal growth factor (EGF) receptor autophosphorylation sites in the regulation of receptor functions has been studied using cells transfected with mutant EGF receptors. Simultaneous point mutation of 4 tyrosines (Y1068, Y1086, Y1148, Y1173) to phenylalanine, as well as removal of ...

  8. Intra-myocardial injection of both growth factors and heart derived Sca-1+/CD31- cells attenuates post-MI LV remodeling more than does cell transplantation alone: neither intervention enhances functionally significant cardiomyocyte regeneration.

    Directory of Open Access Journals (Sweden)

    Xiaohong Wang

    Full Text Available Insulin-like growth factor 1 (IGF-1 and hepatocyte growth factor (HGF are two potent cell survival and regenerative factors in response to myocardial injury (MI. We hypothesized that simultaneous delivery of IGF+HGF combined with Sca-1+/CD31- cells would improve the outcome of transplantation therapy in response to the altered hostile microenvironment post MI. One million adenovirus nuclear LacZ-labeled Sca-1+/CD31- cells were injected into the peri-infarction area after left anterior descending coronary artery (LAD ligation in mice. Recombinant mouse IGF-1+HGF was added to the cell suspension prior to the injection. The left ventricular (LV function was assessed by echocardiography 4 weeks after the transplantation. The cell engraftment, differentiation and cardiomyocyte regeneration were evaluated by histological analysis. Sca-1+/CD31- cells formed viable grafts and improved LV ejection fraction (EF (Control, 54.5+/-2.4; MI, 17.6+/-3.1; Cell, 28.2+/-4.2, n = 9, P<0.01. IGF+HGF significantly enhanced the benefits of cell transplantation as evidenced by increased EF (38.8+/-2.2; n = 9, P<0.01 and attenuated adverse structural remodeling. Furthermore, IGF+HGF supplementation increased the cell engraftment rate, promoted the transplanted cell survival, enhanced angiogenesis, and minimally stimulated endogenous cardiomyocyte regeneration in vivo. The in vitro experiments showed that IGF+HGF treatment stimulated Sca-1+/CD31- cell proliferation and inhibited serum free medium induced apoptosis. Supperarray profiling of Sca-1+/CD31- cells revealed that Sca-1+/CD31- cells highly expressed various trophic factor mRNAs and IGF+HGF treatment altered the mRNAs expression patterns of these cells. These data indicate that IGF-1+HGF could serve as an adjuvant to cell transplantation for myocardial repair by stimulating donor cell and endogenous cardiac stem cell survival, regeneration and promoting angiogenesis.

  9. Distribution of nitric oxide synthase, nerve growth factor receptor and interstitial cells of Cajal in hirschsprung s disease and its significance

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Objective To investigate the distribution of nitric oxide synthase (NOS), nerve growth factor receptor (NGFR) and interstitial cells of Cajal (ICCs) in Hirschsprung's disease (HD). Methods The distribution of NOS, NGFR and ICCs was studied by using NADPH diaphorase histochemistry, immunohistochemistry with a monoclonal antibody to human NGFR and the specific polyclonal antibody against c-kit in 8 normal controls and 10 cases of HD. Results NOS and NGFR were abundantly present in the myenteric plexus and in ...

  10. Significance of Haemodynamic and Haemostatic Factors in the Course of Different Manifestations of Cerebral Small Vessel Disease: The SHEF-CSVD Study—Study Rationale and Protocol

    Directory of Open Access Journals (Sweden)

    Jacek Staszewski

    2013-01-01

    Full Text Available Rationale. This paper describes the rationale and design of the SHEF-CSVD Study, which aims to determine the long-term clinical and radiological course of cerebral small vessel disease (CSVD and to evaluate haemostatic and haemodynamic prognostic factors of the condition. Design. This single-centre, prospective, non-interventional cohort study will follow 150 consecutive patients with different clinical manifestations of CSVD (lacunar ischaemic stroke, vascular dementia, vascular parkinsonism or spontaneous deep, intracerebral haemorrhage and 50 age- and sex-matched controls over a period of 24 months. The clinical and radiological course will be evaluated basing on a detailed neurological, neuropsychological and MRI examinations. Haemodynamic (cerebral vasoreactivity, 24 h blood pressure control and haemostatic factors (markers of endothelial and platelet dysfunction, brachial artery flow-mediated dilatation test will be determined. Discussion. The scheduled study will specifically address the issue of haemodynamic and haemostatic prognostic factors and their course over time in various clinical manifestations of CSVD. The findings may aid the development of prophylactic strategies and individualised treatment plans, which are critical during the early stages of the disease.

  11. Genome-Wide Chromatin Immunoprecipitation Sequencing Analysis Shows that WhiB Is a Transcription Factor That Cocontrols Its Regulon with WhiA To Initiate Developmental Cell Division in Streptomyces

    Directory of Open Access Journals (Sweden)

    Matthew J. Bush

    2016-04-01

    Full Text Available WhiB is the founding member of a family of proteins (the WhiB-like [Wbl] family that carry a [4Fe-4S] iron-sulfur cluster and play key roles in diverse aspects of the biology of actinomycetes, including pathogenesis, antibiotic resistance, and the control of development. In Streptomyces, WhiB is essential for the process of developmentally controlled cell division that leads to sporulation. The biochemical function of Wbl proteins has been controversial; here, we set out to determine unambiguously if WhiB functions as a transcription factor using chromatin immunoprecipitation sequencing (ChIP-seq in Streptomyces venezuelae. In the first demonstration of in vivo genome-wide Wbl binding, we showed that WhiB regulates the expression of key genes required for sporulation by binding upstream of ~240 transcription units. Strikingly, the WhiB regulon is identical to the previously characterized WhiA regulon, providing an explanation for the identical phenotypes of whiA and whiB mutants. Using ChIP-seq, we demonstrated that in vivo DNA binding by WhiA depends on WhiB and vice versa, showing that WhiA and WhiB function cooperatively to control expression of a common set of WhiAB target genes. Finally, we show that mutation of the cysteine residues that coordinate the [4Fe-4S] cluster in WhiB prevents DNA binding by both WhiB and WhiA in vivo.

  12. Human NR5A1/SF-1 mutations show decreased activity on BDNF (brain-derived neurotrophic factor), an important regulator of energy balance: testing impact of novel SF-1 mutations beyond steroidogenesis.

    Science.gov (United States)

    Malikova, Jana; Camats, Núria; Fernández-Cancio, Mónica; Heath, Karen; González, Isabel; Caimarí, María; del Campo, Miguel; Albisu, Marian; Kolouskova, Stanislava; Audí, Laura; Flück, Christa E

    2014-01-01

    Human NR5A1/SF-1 mutations cause 46,XY disorder of sex development (DSD) with broad phenotypic variability, and rarely cause adrenal insufficiency although SF-1 is an important transcription factor for many genes involved in steroidogenesis. In addition, the Sf-1 knockout mouse develops obesity with age. Obesity might be mediated through Sf-1 regulating activity of brain-derived neurotrophic factor (BDNF), an important regulator of energy balance in the ventromedial hypothalamus. To characterize novel SF-1 gene variants in 4 families, clinical, genetic and functional studies were performed with respect to steroidogenesis and energy balance. 5 patients with 46,XY DSD were found to harbor NR5A1/SF-1 mutations including 2 novel variations. One patient harboring a novel mutation also suffered from adrenal insufficiency. SF-1 mutations were studied in cell systems (HEK293, JEG3) for impact on transcription of genes involved in steroidogenesis (CYP11A1, CYP17A1, HSD3B2) and in energy balance (BDNF). BDNF regulation by SF-1 was studied by promoter assays (JEG3). Two novel NR5A1/SF-1 mutations (Glu7Stop, His408Profs*159) were confirmed. Glu7Stop is the 4th reported SF-1 mutation causing DSD and adrenal insufficiency. In vitro studies revealed that transcription of the BDNF gene is regulated by SF-1, and that mutant SF-1 decreased BDNF promoter activation (similar to steroid enzyme promoters). However, clinical data from 16 subjects carrying SF-1 mutations showed normal birth weight and BMI. Glu7Stop and His408Profs*159 are novel SF-1 mutations identified in patients with 46,XY DSD and adrenal insufficiency (Glu7Stop). In vitro, SF-1 mutations affect not only steroidogenesis but also transcription of BDNF which is involved in energy balance. However, in contrast to mice, consequences on weight were not found in humans with SF-1 mutations.

  13. Human NR5A1/SF-1 mutations show decreased activity on BDNF (brain-derived neurotrophic factor, an important regulator of energy balance: testing impact of novel SF-1 mutations beyond steroidogenesis.

    Directory of Open Access Journals (Sweden)

    Jana Malikova

    Full Text Available Human NR5A1/SF-1 mutations cause 46,XY disorder of sex development (DSD with broad phenotypic variability, and rarely cause adrenal insufficiency although SF-1 is an important transcription factor for many genes involved in steroidogenesis. In addition, the Sf-1 knockout mouse develops obesity with age. Obesity might be mediated through Sf-1 regulating activity of brain-derived neurotrophic factor (BDNF, an important regulator of energy balance in the ventromedial hypothalamus.To characterize novel SF-1 gene variants in 4 families, clinical, genetic and functional studies were performed with respect to steroidogenesis and energy balance.5 patients with 46,XY DSD were found to harbor NR5A1/SF-1 mutations including 2 novel variations. One patient harboring a novel mutation also suffered from adrenal insufficiency.SF-1 mutations were studied in cell systems (HEK293, JEG3 for impact on transcription of genes involved in steroidogenesis (CYP11A1, CYP17A1, HSD3B2 and in energy balance (BDNF. BDNF regulation by SF-1 was studied by promoter assays (JEG3.Two novel NR5A1/SF-1 mutations (Glu7Stop, His408Profs*159 were confirmed. Glu7Stop is the 4th reported SF-1 mutation causing DSD and adrenal insufficiency. In vitro studies revealed that transcription of the BDNF gene is regulated by SF-1, and that mutant SF-1 decreased BDNF promoter activation (similar to steroid enzyme promoters. However, clinical data from 16 subjects carrying SF-1 mutations showed normal birth weight and BMI.Glu7Stop and His408Profs*159 are novel SF-1 mutations identified in patients with 46,XY DSD and adrenal insufficiency (Glu7Stop. In vitro, SF-1 mutations affect not only steroidogenesis but also transcription of BDNF which is involved in energy balance. However, in contrast to mice, consequences on weight were not found in humans with SF-1 mutations.

  14. An Evaluation of the Significance of Work-Related Influence Factors on Fitness and the Development of Medical and Orthopaedic Conditions in Military Executives

    Science.gov (United States)

    Becker, Michael; Finze, Susanne; Holtherm, Christoph; Hinder, Jens; Lison, Andreas

    2016-01-01

    Occupational health promotion is an effective tool to improve the state of health of employees. As part of occupational health promotion in the German Bundeswehr, top-ranking military executives are offered a medical examination and training programme. Health-related data is collected as a basis for training and lifestyle counselling. This data was subjected to a retrospective evaluation in order to identify occupational risk factors and their correlation with cardiovascular resilience, trunk strength, and the development of orthopaedic and internal disorders. A total of 122 military executives (all male, age 54.6 ± 4.2 years) answered a questionnaire aimed at evaluating private and occupational stress factors. The medical history was followed by a medical and orthopaedic examination involving a lactate performance test (treadmill or bicycle ergometry) and an isometric trunk strength measurement. The data obtained was then statistically evaluated. For military executives, work-related travelling and commuting involve a high risk of medical and orthopaedic conditions. Regular exercise leads to improved fitness levels. In order to prevent medical problems, military executives working long hours should regularly take part in fitness and weight training under professional instructions.

  15. An Evaluation of the Significance of Work-Related Influence Factors on Fitness and the Development of Medical and Orthopaedic Conditions in Military Executives

    Directory of Open Access Journals (Sweden)

    Christoph Schulze

    2016-01-01

    Full Text Available Occupational health promotion is an effective tool to improve the state of health of employees. As part of occupational health promotion in the German Bundeswehr, top-ranking military executives are offered a medical examination and training programme. Health-related data is collected as a basis for training and lifestyle counselling. This data was subjected to a retrospective evaluation in order to identify occupational risk factors and their correlation with cardiovascular resilience, trunk strength, and the development of orthopaedic and internal disorders. A total of 122 military executives (all male, age 54.6±4.2 years answered a questionnaire aimed at evaluating private and occupational stress factors. The medical history was followed by a medical and orthopaedic examination involving a lactate performance test (treadmill or bicycle ergometry and an isometric trunk strength measurement. The data obtained was then statistically evaluated. For military executives, work-related travelling and commuting involve a high risk of medical and orthopaedic conditions. Regular exercise leads to improved fitness levels. In order to prevent medical problems, military executives working long hours should regularly take part in fitness and weight training under professional instructions.

  16. Monoclonal gammopathy of undetermined significance (MGUS) and smoldering (asymptomatic) multiple myeloma: IMWG consensus perspectives risk factors for progression and guidelines for monitoring and management

    NARCIS (Netherlands)

    R. Kyle (Robert); B.G.M. Durie (Brian); S.V. Rajkumar (Vincent); O. Landgren; J. Bladé (Joan); G. Merlini; N. Kröger; H. Einsele (Hermann); D. Vesole (David); M.A. Dimopoulos (Meletios); J.F. San Miguel (Jesús Fernando); H. Avet-Loiseau; R. Hajek (Roman); W. Chen (Wei); K.C. Anderson (Kenneth Carl); H. Ludwig (Heinz); P. Sonneveld (Pieter); S. Pavlovsky; A. Palumbo (Antonio); P.G. Richardson; B. Barlogie (Bart); P. Greipp (Philip); R. Vescio (Robert); I. Turesson; J. Westin (Johan); M. Boccadoro (Mario)

    2010-01-01

    textabstractMonoclonal gammopathy of undetermined significance (MGUS) was identified in 3.2% of 21 463 residents of Olmsted County, Minnesota, 50 years of age or older. The risk of progression to multiple myeloma, Waldenstrom's macroglobulinemia, AL amyloidosis or a lymphoproliferative disorder is a

  17. Monoclonal gammopathy of undetermined significance (MGUS) and smoldering (asymptomatic) multiple myeloma: IMWG consensus perspectives risk factors for progression and guidelines for monitoring and management

    NARCIS (Netherlands)

    R. Kyle (Robert); B.G.M. Durie (Brian); S.V. Rajkumar (Vincent); O. Landgren; J. Bladé (Joan); G. Merlini; N. Kröger; H. Einsele (Hermann); D. Vesole (David); M.A. Dimopoulos (Meletios); J.F. San Miguel (Jesús Fernando); H. Avet-Loiseau; R. Hajek (Roman); W. Chen (Wei); K.C. Anderson (Kenneth Carl); H. Ludwig (Heinz); P. Sonneveld (Pieter); S. Pavlovsky; A. Palumbo (Antonio); P.G. Richardson; B. Barlogie (Bart); P. Greipp (Philip); R. Vescio (Robert); I. Turesson; J. Westin (Johan); M. Boccadoro (Mario)

    2010-01-01

    textabstractMonoclonal gammopathy of undetermined significance (MGUS) was identified in 3.2% of 21 463 residents of Olmsted County, Minnesota, 50 years of age or older. The risk of progression to multiple myeloma, Waldenstrom's macroglobulinemia, AL amyloidosis or a lymphoproliferative disorder is a

  18. Monoclonal gammopathy of undetermined significance (MGUS) and smoldering (asymptomatic) multiple myeloma: IMWG consensus perspectives risk factors for progression and guidelines for monitoring and management

    NARCIS (Netherlands)

    R. Kyle (Robert); B.G.M. Durie (Brian); S.V. Rajkumar (Vincent); O. Landgren; J. Bladé (Joan); G. Merlini; N. Kröger; H. Einsele (Hermann); D. Vesole (David); M.A. Dimopoulos (Meletios); J.F. San Miguel (Jesús Fernando); H. Avet-Loiseau; R. Hajek (Roman); W. Chen (Wei); K.C. Anderson (Kenneth Carl); H. Ludwig (Heinz); P. Sonneveld (Pieter); S. Pavlovsky; A. Palumbo (Antonio); P.G. Richardson; B. Barlogie (Bart); P. Greipp (Philip); R. Vescio (Robert); I. Turesson; J. Westin (Johan); M. Boccadoro (Mario)

    2010-01-01

    textabstractMonoclonal gammopathy of undetermined significance (MGUS) was identified in 3.2% of 21 463 residents of Olmsted County, Minnesota, 50 years of age or older. The risk of progression to multiple myeloma, Waldenstrom's macroglobulinemia, AL amyloidosis or a lymphoproliferative disorder is

  19. Granulocyte colony-stimulating factor-producing undifferentiated carcinoma of the colon mimicking a pulmonary giant cell carcinoma: a case showing overexpression of CD44 along with highly proliferating nestin-positive tumor vessels.

    Science.gov (United States)

    Tajima, Shogo; Waki, Michihiko; Tsuchiya, Tomonori; Hoshi, Shoji

    2014-01-01

    Granulocyte colony-stimulating factor (G-CSF)-producing tumors are known for their aggressive behavior. Only four cases of G-CSF-producing colorectal carcinoma have been previously reported. Herein, we present a case of an undifferentiated carcinoma of the descending colon showing G-CSF production and giant cell carcinoma morphology in a 93-year-old woman. A tumor with a diameter of 80 mm was identified in the descending colon via computed tomography. Descending colectomy was performed involving the abdominal wall where tumor invasion was observed. The white blood cell count, which was elevated before resection, decreased to normal levels after intervention. However, local recurrence at the resected site was detected 39 days after surgery. Upon recurrence, increased white blood cell counts and serum G-CSF were seen. The patient died because of respiratory failure 98 days after colectomy. By using immunohistochemistry, G-CSF expression was detected in tumor cells in the resected specimen, along with overexpression of CD44 and highly proliferating nestin-positive tumor vessels. The poor clinical outcome of this patient is consistent with previous reports that the expression of these three molecules predict poor prognosis. While G-CSF can be a therapeutic target considering its auto/paracrine function to induce tumor growth via the G-CSF receptor, CD44 and nestin may also be possible candidate therapeutic targets. Further studies are required to assess the efficacy of treatments targeting these three molecules.

  20. Stunting, poor iron status and parasite infection are significant risk factors for lower cognitive performance in Cambodian school-aged children

    DEFF Research Database (Denmark)

    Perignon, Marlene; Fiorentino, Marion; Khov, Kuong

    2014-01-01

    using Raven's Colored Progressive Matrices (RCPM) and block design and picture completion, two standardized tests from the Wechsler Intelligence Scale for Children (WISC-III). RESULTS: The prevalence of anemia, iron, zinc, iodine and vitamin A deficiency were 15.7%; 51.2%, 92.8%, 17.3% and 0......BACKGROUND: Nutrition is one of many factors affecting the cognitive development of children. In Cambodia, 55% of children children, or on how malnutrition potentially affects...... their cognitive development. OBJECTIVE: To assess the anthropometric and micronutrient status (iron, vitamin A, zinc, iodine) of Cambodian schoolchildren and their associations with cognitive performance. METHODS: School children aged 6-16 y (n = 2443) from 20 primary schools in Cambodia were recruited...

  1. Expression and significance of E-cadherin, N-cadherin, transforming growth factor-β1 and Twist in prostate cancer

    Institute of Scientific and Technical Information of China (English)

    Ge-Liang Liu; Han-Jie Yang; Tian Liu; Yun-Zhao Lin

    2014-01-01

    Objective: To study the expression of E-cadherin, N-cadherin, TGF-1 and Twist protein and investigate its significance in the occurrence and development of prostate cancer. Methods:The expression of E-cadherin, N-cadherin, TGF-1 and Twist protein in 59 prostate cancer tissues and 21 adjacent tissues were detected by immunohistochemical SABC staining, and the correlation with clinicopathological features was analyzed. Results: Positive rates of E-cadherin, N-cadherin, TGF-1 and Twist were 32.2%, 54.2%, 71.2% and 74.6%, respectively, in prostate cancer tissues and 85.7%, 9.52%, 19.0% and 9.52%, respectively, in cancer-adjacent tissues, with significant differences between the two groups (P20g/L group, but the positive expression of Twist was not significant between groups. The expression of E-cadherin was highly negatively correlated with that of N-cadherin and also highly negatively correlated with that of Twist. The expression of TGF-1 was correlated with those of E-cadherin, N-cadherin and Twist. Conclusions: The reduced expression of E-cadherin, abnormal expression of N-cadherin, transformation form E-cadherin to N-cadherin and the increased expression of TGF-1 and Twist play an important role in the occurrence and development of prostate cancer.

  2. Fragile X premutation is a significant risk factor for premature ovarian failure: the International Collaborative POF in Fragile X study--preliminary data.

    Science.gov (United States)

    Allingham-Hawkins, D J; Babul-Hirji, R; Chitayat, D; Holden, J J; Yang, K T; Lee, C; Hudson, R; Gorwill, H; Nolin, S L; Glicksman, A; Jenkins, E C; Brown, W T; Howard-Peebles, P N; Becchi, C; Cummings, E; Fallon, L; Seitz, S; Black, S H; Vianna-Morgante, A M; Costa, S S; Otto, P A; Mingroni-Netto, R C; Murray, A; Webb, J; Vieri, F

    1999-04-01

    The preliminary results of an international collaborative study examining premature menopause in fragile X carriers are presented. A total of 760 women from fragile X families was surveyed about their fragile X carrier status and their menstrual and reproductive histories. Among the subjects, 395 carried a premutation, 128 carried a full mutation, and 237 were noncarriers. Sixty-three (16%) of the premutation carriers had experienced menopause prior to the age of 40 compared with none of the full mutation carriers and one (0.4%) of the controls. Based on these preliminary data, there is a significant association between fragile X premutation carrier status and premature menopause.

  3. Sterol regulatory element binding transcription factor 1 expression and genetic polymorphism significantly affect intramuscular fat deposition in the longissimus muscle of Erhualian and Sutai pigs.

    Science.gov (United States)

    Chen, J; Yang, X J; Xia, D; Chen, J; Wegner, J; Jiang, Z; Zhao, R Q

    2008-01-01

    Two experiments were performed to elucidate the role of sterol regulatory element binding transcription factor 1 (SREBF1) in i.m. fat (IMF) deposition in pigs. In Exp. 1, LM samples were removed from 4 male and 4 female Erhualian piglets at 3, 20, and 45 d of age, and SREBF1 mRNA expression level and IMF content were measured. Intramuscular fat content and expression of SREBF1 mRNA was greater (P Single-strand conformation polymorphism (SSCP) analysis of the reverse transcription PCR products of the SREBF1 gene revealed 3 genotypes in Sutai pigs with frequencies of 50% for AA, 36% for AB, and 14% for BB, respectively. Both SREBF1 mRNA level and IMF content in muscle were greater (P < 0.05) in AB and BB animals than in AA animals, whereas no difference in backfat thickness was observed among the 3 genotypes. Sequencing analysis identified 2 SNP at T1006C and C1033T within the open reading frame of the SREBF1 gene (NM_214157). Although both are silent mutations, they affected the secondary structure of SREBF1 mRNA. These results suggest that SREBF1 might play an important role in regulation of muscle fat deposition during postnatal growth of pigs. The SNP identified in the SREBF1 gene suggest that it could be used as a genetic marker to improve IMF content in pigs.

  4. Significance of yeasts in bloodstream infection: Epidemiology and predisposing factors of Candidaemia in adult patients at a university hospital (2010-2014).

    Science.gov (United States)

    Pongrácz, Júlia; Juhász, Emese; Iván, Miklós; Kristóf, Katalin

    2015-09-01

    The incidence of Candida bloodstream infection (BSI) has increased during the past decades. Species distribution is changing worldwide, and non-albicans Candida spp. are becoming more prevalent. Acquired resistance to antifungal agents has been documented in several reports. The aim of our study was to assess the epidemiology and antifungal susceptibility of Candida isolates from BSI at our institute. The incidence of Candida BSI increased during the first four years of our investigation, from 1.7 to 3.5 episodes / 10 000 admissions, then dropped to 2.66 episodes / 10 000 admissions in the last year. The most frequently isolated species was C. albicans (63%), followed by C. glabrata (13%), C. parapsilosis (10.2%), C. tropicalis (9.3%), and C. krusei (3.7%). One isolate each of C. kefyr, C. fabianii and C. inconspicua were detected. The percentage of C. albicans remained stable throughout the study period. The most frequent risk factors of Candida BSI in our patient population were intensive care treatment (60.4%), abdominal surgery (52.5%), and solid malignancy (30.7%). All isolates were wild-type organisms, no acquired antifungal resistance was detected.

  5. 大肠癌中Pokemon的表达及其临床意义%Expression of transcription factor Pokemon in colorectal cancer and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    王鹏飞; 吴静

    2009-01-01

    目的:探讨原癌基因Pokemon(POK erythroid mye-loid ontogenic)在大肠癌组织中的表达及其与临床特征之间的关系.方法:采用逆转录酶聚合酶链反应(RT-PCR)方法检测47例大肠癌患者癌、癌旁组织、癌以远大肠组织中Pokemon mRNA的表达水平,分析其与临床特征之间的关系;采用免疫组化ABC法检测53例大肠癌组织中Pokemon蛋白的表达,结合随访资料分析其与患者生存时间的关系.结果:大肠癌癌组织和癌旁组织中Pokemon mRNA水平显著高于正常大肠组织(P<0.05),而癌与癌旁组织中的表达无显著性差异;Pokemon mRNA的表达水平与大肠癌Dukes分期、分化程度及淋巴结转移相关(P<0.05),而与患者的性别、年龄、吸烟、饮酒、肿瘤位置、大小及浸润深度无关;Pokemon表达阴性的大肠癌患者存活率明显高于阳性表达患者(P<0.05).结论:Poke-inon在大肠癌和癌旁组织中高表达,并对大肠癌的预后评价有重要意义,且可能成为大肠癌治疗中有效的新的靶基因.%AIM: To investigate the expression and significance of Pokemon(POK erythroid myeloid ontogenic) proto-oncogene in colorectal cancer. METHODS: The expression of Pokemon in the tumor tissues, corresponding tumor adjacent tissues and the surrounding tissues collected from 47 operation-treated cases of coloreetal cancers was detected by reverse transcription-polymerase chain reaction(RT-PCR), and the clinical and histopathologic records were retrospectively analyzed. The expression of Pokemon protein was detected by immunohistochemical(IHC) techniques in 53 cases of colorectal cancer to explore its relation with the survival time of the patients. RESULTS: The expression of Pokemort mRNA in coloreetal tumor and corresponding tumor adjacent tissues, with no significant difference between them (P=0.054), was markedly higher than that in the surrounding tissues (P<0.05). It was correlated with Dukes staging, tumor differentiation

  6. Epithelial - Mesenchymal Interactions in Tooth Development and the Significant Role of Growth Factors and Genes with Emphasis on Mesenchyme - A Review.

    Science.gov (United States)

    Puthiyaveetil, Jaya Sekharan Vannadil; Kota, Kasim; Chakkarayan, Roopesh; Chakkarayan, Jithesh; Thodiyil, Abdul Kabeer Padinhare

    2016-09-01

    The recent advancements in medical research field mainly highlights the genetic and molecular aspects of various disease processes and related treatment options, in a specialized "custom-made" approach. The medical and dental field has made tremendous progress in providing even with the smallest insight into pathological entities, thus, making patient management more fruitful. But, short comings have occurred in dental treatments involving odontogenic lesions mainly due to poor understanding of the developmental cycle involved during early stages of developmental process. Multiple numbers of interactions take place during embryo formation and further proliferation of tissue. One such important step is the interaction between epithelium and mesenchyme which tantamount to functional requirements of an individual tooth. The role of extra cellular molecules and genes has to be studied in depth to assess the impact and significance attached to it as the synergistic function of various elements underlines the complex process of development.

  7. Epithelial – Mesenchymal Interactions in Tooth Development and the Significant Role of Growth Factors and Genes with Emphasis on Mesenchyme – A Review

    Science.gov (United States)

    Kota, Kasim; Chakkarayan, Roopesh; Chakkarayan, Jithesh; Thodiyil, Abdul Kabeer Padinhare

    2016-01-01

    The recent advancements in medical research field mainly highlights the genetic and molecular aspects of various disease processes and related treatment options, in a specialized “custom-made” approach. The medical and dental field has made tremendous progress in providing even with the smallest insight into pathological entities, thus, making patient management more fruitful. But, short comings have occurred in dental treatments involving odontogenic lesions mainly due to poor understanding of the developmental cycle involved during early stages of developmental process. Multiple numbers of interactions take place during embryo formation and further proliferation of tissue. One such important step is the interaction between epithelium and mesenchyme which tantamount to functional requirements of an individual tooth. The role of extra cellular molecules and genes has to be studied in depth to assess the impact and significance attached to it as the synergistic function of various elements underlines the complex process of development. PMID:27790596

  8. Identification of the significant factors in food safety using global sensitivity analysis and the accept-and-reject algorithm: application to the cold chain of ham.

    Science.gov (United States)

    Duret, Steven; Guillier, Laurent; Hoang, Hong-Minh; Flick, Denis; Laguerre, Onrawee

    2014-06-16

    Deterministic models describing heat transfer and microbial growth in the cold chain are widely studied. However, it is difficult to apply them in practice because of several variable parameters in the logistic supply chain (e.g., ambient temperature varying due to season and product residence time in refrigeration equipment), the product's characteristics (e.g., pH and water activity) and the microbial characteristics (e.g., initial microbial load and lag time). This variability can lead to different bacterial growth rates in food products and has to be considered to properly predict the consumer's exposure and identify the key parameters of the cold chain. This study proposes a new approach that combines deterministic (heat transfer) and stochastic (Monte Carlo) modeling to account for the variability in the logistic supply chain and the product's characteristics. The model generates a realistic time-temperature product history , contrary to existing modeling whose describe time-temperature profile Contrary to existing approaches that use directly a time-temperature profile, the proposed model predicts product temperature evolution from the thermostat setting and the ambient temperature. The developed methodology was applied to the cold chain of cooked ham including, the display cabinet, transport by the consumer and the domestic refrigerator, to predict the evolution of state variables, such as the temperature and the growth of Listeria monocytogenes. The impacts of the input factors were calculated and ranked. It was found that the product's time-temperature history and the initial contamination level are the main causes of consumers' exposure. Then, a refined analysis was applied, revealing the importance of consumer behaviors on Listeria monocytogenes exposure.

  9. [Predictive factors of clinically significant drug-drug interactions among regimens based on protease inhibitors, non-nucleoside reverse transcriptase inhibitors and raltegravir].

    Science.gov (United States)

    Cervero, Miguel; Torres, Rafael; Jusdado, Juan José; Pastor, Susana; Agud, Jose Luis

    2016-04-15

    To determine the prevalence and types of clinically significant drug-drug interactions (CSDI) in the drug regimens of HIV-infected patients receiving antiretroviral treatment. retrospective review of database. Centre: Hospital Universitario Severo Ochoa, Infectious Unit. one hundred and forty-two participants followed by one of the authors were selected from January 1985 to December 2014. from their outpatient medical records we reviewed information from the last available visit of the participants, in relation to HIV infection, comorbidities, demographics and the drugs that they were receiving; both antiretroviral drugs and drugs not related to HIV infection. We defined CSDI from the information sheet and/or database on antiretroviral drug interactions of the University of Liverpool (http://www.hiv-druginteractions.org) and we developed a diagnostic tool to predict the possibility of CSDI. By multivariate logistic regression analysis and by estimating the diagnostic performance curve obtained, we identified a quick tool to predict the existence of drug interactions. Of 142 patients, 39 (29.11%) had some type of CSDI and in 11.2% 2 or more interactions were detected. In only one patient the combination of drugs was contraindicated (this patient was receiving darunavir/r and quetiapine). In multivariate analyses, predictors of CSDI were regimen type (PI or NNRTI) and the use of 3 or more non-antiretroviral drugs (AUC 0.886, 95% CI 0.828 to 0.944; P=.0001). The risk was 18.55 times in those receiving NNRTI and 27,95 times in those receiving IP compared to those taking raltegravir. Drug interactions, including those defined as clinically significant, are common in HIV-infected patients treated with antiretroviral drugs, and the risk is greater in IP-based regimens. Raltegravir-based prescribing, especially in patients who receive at least 3 non-HIV drugs could avoid interactions. Copyright © 2016 Elsevier España, S.L.U. All rights reserved.

  10. Chicken granulosa cells show differential expression of epidermal growth factor (EGF) and luteinizing hormone (LH) receptor messenger RNA and differential responsiveness to EGF and LH dependent upon location of granulosa cells to the germinal disc.

    Science.gov (United States)

    Yao, H H; Bahr, J M

    2001-06-01

    Granulosa cells in the chicken follicle exhibit different phenotypes according to their location relative to the germinal disc (GD). Granulosa cells proximal to the GD (referred to as proximal granulosa cells) are more proliferative, whereas granulosa cells distal to the GD (referred to as distal granulosa cells) are more differentiated. We have shown that epidermal growth factor (EGF) derived from the GD stimulated proliferation of granulosa cells proximal to the GD, whereas extraovarian LH promoted differentiation. We tested the hypothesis that phenotypic differences of granulosa cells are the result of differential responsiveness of granulosa cells to EGF and LH. We found that both granulosa and theca layers of chicken preovulatory follicles expressed mRNA for EGF receptor (EGFr) by polymerase chain reaction (PCR) analysis. However, only the granulosa layer showed differential expression of EGFr and LH receptor (LHr) mRNA. Competitive reverse transcription-PCR revealed that proximal granulosa cells expressed more EGFr mRNA but less LHr mRNA than distal granulosa cells. In addition, proximal granulosa cells proliferated more in response to EGF than their distal counterparts. We further demonstrated that EGF decreased LHr mRNA expression by granulosa cells in a dose-dependent manner, whereas EGF and LH had no effect on EGFr mRNA expression except at one dose of LH (15 ng/ml) that stimulated EGFr mRNA expression. Our findings suggest that EGF derived from the GD influences the phenotypes of granulosa cells. Granulosa cells proximal to the GD exhibit a proliferative phenotype possibly because they are exposed to and are more responsive to GD-derived EGF. Furthermore, GD-derived EGF decreases LHr mRNA expression by proximal granulosa cells and therefore results in less differentiated granulosa cell phenotype. In contrast, granulosa cells distal to the GD are not under the influence of EGF and exhibit a more differentiated phenotype.

  11. The timing of bowel preparation before colonoscopy determines the quality of cleansing, and is a significant factor contributing to the detection of flat lesions: A randomized study

    Institute of Scientific and Technical Information of China (English)

    Adolfo Parra-Blanco; David Nicolás-Pérez; Antonio Gimeno-García; Bego(n)a Grosso; Alejandro Jiménez; Juan Ortega; Enrique Quintero

    2006-01-01

    AIM: To compare the cleansing quality of polyethylene glycol electrolyte solution and sodium phosphate with different schedules of administration, and to evaluate whether the timing of the administration of bowel preparation affects the detection of polyps.METHODS: One hundred and seventy-seven consecutive outpatients scheduled for colonoscopy were randomized in one of four groups to receive polyethylene glycol electrolyte solution or oral sodium phosphate with two different timing schedules. Quality of cleansing, polyp detection, and tolerance were evaluated.RESULTS: Patients receiving polyethylene glycol or sodium phosphate on the same day as the colonoscopy,obtained good to excellent global cleansing scores more frequently than patients who received polyethylene glycol or sodium phosphate on the day prior to the procedure (P< 0.001). Flat lesions, but not flat adenomas, were more frequent in patients prepared on the same day (P = 0.02).CONCLUSION: The quality of colonic cleansing and the detection of flat lesions are significantly improved when the preparation is taken on the day of the colonoscopy.

  12. Expression and significance of nuclear factor κB p65 in colon tissues of rats with TNBS-induced colitis

    Institute of Scientific and Technical Information of China (English)

    Jun-Hua Li; Jie-Ping Yu; Hong-Gang Yu; Xi-Ming Xu; Liang-Liang Yu; Shi-Quan Liu

    2005-01-01

    AIM: To investigate the role of NF-κB in the pathogenesis of TNBS-induced colitis in rats.METHODS: Thirty-two healthy adult Sprague-Dawley (SD)rats were randomly divided into four groups of eight each:normal, NS, model I, model Ⅱ groups in our study. Rat colitis model was established through 2-,4-,6-trinitrobenzene sulfonic acid (TNBS) enema. At the end of four weeks,the macroscopical and histological changes of the colon were examined and mucosa myeloperoxidase (MPO)activities assayed. NF-κB p65 expression was determined by Western blot assessment in cytoplasmic and nuclear extracts of colon tissue, and the expressions of TNF-αand ICAM-1 protein in colon tissue were examined by immunohistochemistry. The relativities between expression of NF-κBp65 and other parameters were analyzed.RESULTS: TNBS enema resulted in pronounced pathological changes of colonic mucosa in model Ⅱ group (macroscopic and histological injury indices 6.25±1.39 and 6.24±1.04,respectively), which were in accordance with the significantly elevated MPO activity (1.69±0.11). And the nuclear level of NF-κB and expression of TNF-α, ICAM-1 in rats of model Ⅱ group were higher than that of normal control (9.7±1.96 vs1.7±0.15, 84.09±14.52 vs16.03±6.21,77.69±8.09 vs13.41±4.91 P<0.01), Linear correlation analysis revealed that there were strong correlations between the nuclear level of NF-κB and the tissue positive expression of TNF-α and ICAM-1, MPO activities,macroscopical and histological indices in TNBS-induced colitis, respectively (r = 0.8235, 0.8780, 0.8572, 0.9152,0.8247; P<0.05).CONCLUSION: NF-κB plays a pivotal role in the pathogenesis of ulcerative colitis, which might account for the up-regulation the expression of TNF-α and ICAM-1.

  13. Response to Ponamgi et al. Comments on Khalighi et al. Takotsubo Cardiomyopathy: A Long Term Follow-up Shows Benefit with Risk Factor Reduction. J. Cardiovasc. Dev. Dis., 2015, 2, 273–281.

    Directory of Open Access Journals (Sweden)

    Mohammad Umar Farooq

    2016-01-01

    Full Text Available We appreciate the thorough response given by Ponagmi et al. [1], who rightly point out that the pathophysiology and modifiable risk factors of Takotsubo Cardiomyopathy (TC have yet to be unequivocally established. [...

  14. Change and significance of serum fat factors and inflammatory cytokines in children with metabolic syndrome%儿童代谢综合征血清脂肪因子和炎症因子的变化及意义

    Institute of Scientific and Technical Information of China (English)

    宫相翠; 李玉芬

    2016-01-01

    Objective To explore the change and significance of serum fat factors and inflammatory cytokines in children with metabolic syndrome (MS). Methods In this study 50 metabolic syndrome children (MS group), 50 simple obesity children (simple obesity group) and 50 normal children having physical examination (normal control group) were included. They all accepted regular physical examination and biochemical examination. The serum fat factors including adiponectin and leptin and serum inflammatory factors including tumor necrosis factor alpha (TNF-α), hypersensitive C-reactive protein (hs-CRP) and interleukin 6 (IL-6) were checked with double resistant sandwich enzyme-linked immunosorbent assay (ELISA). The correlations of serum fat factors and inflammatory factors with physical and biochemical test results were analyzed. Results The levels of FBG, TC, TG, LDL-C, SBP, DBP, BMI, TNF-α, hs-CRP and IL-6, and waist-to hip ratio in the MS group were significantly higher while the levels of HDL-C and adiponectin were significantly lower than those of the simple obesity group and the normal control group ( 0.05). Correlation analysis showed that the serum hs-CRP was positively correlated to TC, SBP, BMI and waist-to-hip ratio; the serum IL-6 was positively correlated to TC, DBP, BMI and waist-to-hip ratio; and the adiponectin was negatively correlated to TC and LDL-C. Conclusions Serum fat factors and inflammatory factors can be used as the indicators of occurrence and development of MS in children.%目的:探讨儿童代谢综合征(MS)血清脂肪因子和炎症因子的变化及意义。方法收集50例MS患儿(MS组),50例单纯性肥胖儿童(单纯性肥胖组)和50例体格检查正常儿童(正常对照组),对3组儿童进行常规体格检查和生化检查,双抗夹心酶联免疫吸附法测定血清脂肪因子包括脂联素和瘦素以及血清炎症因子包括肿瘤坏死因子-α(TNF-α)、超敏C反应蛋白(hs- CRP

  15. Insulin-like growth factor-binding protein 2-driven glioma progression is prevented by blocking a clinically significant integrin, integrin-linked kinase, and NF-κB network

    Science.gov (United States)

    Holmes, Kristen M.; Annala, Matti; Chua, Corrine Y. X.; Dunlap, Sarah M.; Liu, Yuexin; Hugen, Niek; Moore, Lynette M.; Cogdell, David; Hu, Limei; Nykter, Matti; Hess, Kenneth; Fuller, Gregory N.; Zhang, Wei

    2012-01-01

    Insulin-like growth factor-binding protein 2 (IGFBP2) is increasingly recognized as a glioma oncogene, emerging as a target for therapeutic intervention. In this study, we used an integrative approach to characterizing the IGFBP2 network, combining transcriptional profiling of human glioma with validation in glial cells and the replication-competent ASLV long terminal repeat with a splice acceptor/tv-a glioma mouse system. We demonstrated that IGFBP2 expression is closely linked to genes in the integrin and integrin-linked kinase (ILK) pathways and that these genes are associated with prognosis. We further showed that IGFBP2 activates integrin β1 and downstream invasion pathways, requires ILK to induce cell motility, and activates NF-κB. Most significantly, the IGFBP2/integrin/ILK/NF-κB network functions as a physiologically active signaling pathway in vivo by driving glioma progression; interfering with any point in the pathway markedly inhibits progression. The results of this study reveal a signaling pathway that is both targetable and highly relevant to improving the survival of glioma patients. PMID:22345562

  16. Significance of vascular endothelial growth factor and CD31 and morphometric analysis of microvessel density by CD31 receptor expression as an adjuvant tool in diagnosis of psoriatic lesions of skin.

    Science.gov (United States)

    Chawla, Nitika; Kataria, Sant Prakash; Aggarwal, Kamal; Chauhan, Pardeep; Kumar, Dinesh

    2017-01-01

    Pathogenesis of psoriasis is a debated issue. Several mechanisms have been proposed to identify the etiology and pathogenesis so that specific treatments can be given to patients with psoriasis. (1) To compare pattern and distribution of vascular endothelial growth factor (VEGF) and CD31 in patients with psoriasis and other psoriasiform lesions of skin. (2) To study the correlation between VEGF and CD31 expression, clinical severity, and histopathology of psoriasiform lesions of skin. (3) Evaluation of microvessel density (MVD) by using computer-assisted quantitative image analysis in psoriatic skin lesions. This study was conducted on eighty cases, out of which forty were diagnosed cases of psoriasis and forty cases of clinically suspected psoriasiform lesions, submitted in the Department of Pathology, Pt. B.D. Sharma, University of Health Sciences, Rohtak, for histopathological examination. Histopathological sections were stained by routine hematoxylin and eosin staining, and these biopsies were further subjected to immunohistochemical staining with VEGF and CD31 as per standard technique. Assessment of various histopathological features revealed strong correlation between epidermal hyperplasia, suprapapillary thinning, and elongation of rete ridges. Suprabasilar keratinocytes in psoriatic lesions stained intensely for VEGF. The difference for number of microvessels and MVD in psoriasis and psoriasiform lesions was statistically significant. Correlation between intensity of VEGF staining by suprabasilar keratinocytes and MVD was found to be highly significant in psoriatic lesions. The present study concluded that psoriatic lesions exhibit potent angiogenic activity. Early lesions show increased MVD along with other histomorphological parameters such as hypogranulosis, parakeratosis and Munro's microabscesses. Overexpression of VEGF by suprabasilar keratinocytes correlated with increased MVD in papillary dermis.

  17. Clinical significance of nuclear factor κB and chemokine receptor CXCR4 expression in patients with diffuse large B-cell lymphoma who received rituximab-based therapy.

    Science.gov (United States)

    Shin, Ho Cheol; Seo, Jongwon; Kang, Byung Woog; Moon, Joon Ho; Chae, Yee Soo; Lee, Soo Jung; Lee, Yoo Jin; Han, Seoae; Seo, Sang Kyung; Kim, Jong Gwang; Sohn, Sang Kyun; Park, Tae-In

    2014-11-01

    This study investigated the expression of nuclear factor κB (NF-κB) and the chemokine receptor (CXCR4) in patients with diffuse large B-cell lymphoma (DLBCL) who received rituximab-based therapy. Seventy patients with DLBCL and treated with rituximab-CHOP (R-CHOP) were included, and immunohistochemistry was performed to determine the expression of NF-κB (IκB kinase α, p50, and p100/p52) and CXCR4. To classify DLBCL cases as germinal center B-cell-like (GCB) and non-GCB, additional immunohistochemical expression of CD10, bcl-6, or MUM1 was used in this study. The expression was divided into two groups according to the intensity score (negative, 0 or 1+; positive, 2+ or 3+). The median age of the patients was 66 years (range, 17 to 87), and 58.6% were male. Twenty-seven patients (38.6%) had stage III or IV disease at diagnosis. Twenty-three patients (32.9%) were categorized as high or high-intermediate risk according to their International Prognostic Indexs (IPIs). The overall incidence of bone marrow involvement was 5.7%. Rates of positive NF-κB and CXCR4 expression were 84.2% and 88.6%, respectively. High NF-κB expression was associated with CXCR4 expression (p = 0.002), and 56 patients (80.0%) showed coexpression. However, the expression of NF-κB or CXCR4 was not associated with overall survival and EFS. On multivariate analysis that included age, gender, performance status, stage, and the IPI, no significant association between the grade of NF-κB or CXCR4 expression and survival was observed. The current study suggests that the tissue expression of NF-κB and CXCR4 may not be an independent prognostic marker in DLBCL patients treated with R-CHOP.

  18. Depot medroxyprogesterone acetate in combination with a twice-daily lopinavir-ritonavir-based regimen in HIV-infected women showed effective contraception and a lack of clinically significant interactions, with good safety and tolerability: results of the ACTG 5283 study.

    Science.gov (United States)

    Luque, Amneris E; Cohn, Susan E; Park, Jeong-Gun; Cramer, Yoninah; Weinberg, Adriana; Livingston, Elizabeth; Klingman, Karin L; Aweeka, Francesca; Watts, D Heather

    2015-04-01

    We conducted an open-label, steady-state pharmacokinetic (PK) study of drug-drug interactions between depot medroxyprogesterone acetate (DMPA) and twice-daily lopinavir (LPV) plus low-dose ritonavir (RTV) (LPV/r) among 24 HIV-infected women and compared the results to those for HIV-infected women receiving DMPA while on no antiretroviral therapy or on nucleosides only (n = 14 subjects from the control arm of AIDS Clinical Trials Group [ACTG] study 5093). The objectives of the study were to address the effect of LPV/r on DMPA and to address the effect of DMPA on LPV/r therapy. PK parameters were estimated using noncompartmental analysis with between-group comparisons of medroxyprogesterone acetate (MPA) PKs and within-subject comparisons of LPV and RTV PKs before and 4 weeks after DMPA dosing. Plasma progesterone concentrations were measured every 2 weeks after DMPA dosing through week 12. Although the MPA area under the concentration-time curve and maximum concentration of drug in plasma were statistically significantly increased in the study women on LPV/r compared to those in the historical controls, these increases were not considered clinically significant. There were no changes in LPV or RTV exposure after DMPA. DMPA was well tolerated, and suppression of ovulation was maintained. (This study has been registered at ClinicalTrials.gov under registration no. NCT01296152.). Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  19. A new case of Carney triad: gastrointestinal stromal tumours and leiomyoma of the oesophagus do not show activating mutations of KIT and platelet‐derived growth factor receptor α

    Science.gov (United States)

    Knop, S; Schupp, M; Wardelmann, E; Stueker, D; Horger, M S; Kanz, L; Einsele, H; Kroeber, S M

    2006-01-01

    The Carney triad is a rare syndrome of unknown aetiology, with synchronous or metachronous appearance of rare neoplasms: gastrointestinal stromal tumours (GISTs), pulmonary chondromas and extra‐adrenal paragangliomas. In most cases, the Carney triad is incomplete. The combination encountered typically, GISTs and pulmonary chondromas, was also seen in our patient, a 22‐year‐old woman. She was diagnosed with the triad after Billroth II gastrectomy for histologically proved gastric GISTs. The diagnosis of pulmonary chondromas was confirmed by transthoracic, computed tomography‐guided needle biopsy. An oesophageal leiomyoma was resected 2 years after the initial diagnosis, on suspicion of paraganglioma. The clinical course of the patient has been uneventful since. The last follow‐up was carried out 6 years after the initial diagnosis. On histological examination, the cells of gastric GIST were partly positive for CD34, whereas CD117 was expressed in all areas in variable intensity and S‐100 protein was negative. The oesophageal tumour was classified as leiomyoma due to strong immunopositivity for smooth muscle actin and desmin, being negative for CD34 and CD117. Two different gastric GIST lesions as well as the oesophageal leiomyoma and normal tissue were analysed for activating mutations in common hot spots of KIT (exon 9 and 11) and platelet‐derived growth factor receptor α (exon 18), but in all probes wild‐type sequences were found. These results are in accordance with the first published analyses of GIST lesions from Carney patients. PMID:17021135

  20. A new case of Carney triad: gastrointestinal stromal tumours and leiomyoma of the oesophagus do not show activating mutations of KIT and platelet-derived growth factor receptor alpha.

    Science.gov (United States)

    Knop, S; Schupp, M; Wardelmann, E; Stueker, D; Horger, M S; Kanz, L; Einsele, H; Kroeber, S M

    2006-10-01

    The Carney triad is a rare syndrome of unknown aetiology, with synchronous or metachronous appearance of rare neoplasms: gastrointestinal stromal tumours (GISTs), pulmonary chondromas and extra-adrenal paragangliomas. In most cases, the Carney triad is incomplete. The combination encountered typically, GISTs and pulmonary chondromas, was also seen in our patient, a 22-year-old woman. She was diagnosed with the triad after Billroth II gastrectomy for histologically proved gastric GISTs. The diagnosis of pulmonary chondromas was confirmed by transthoracic, computed tomography-guided needle biopsy. An oesophageal leiomyoma was resected 2 years after the initial diagnosis, on suspicion of paraganglioma. The clinical course of the patient has been uneventful since. The last follow-up was carried out 6 years after the initial diagnosis. On histological examination, the cells of gastric GIST were partly positive for CD34, whereas CD117 was expressed in all areas in variable intensity and S-100 protein was negative. The oesophageal tumour was classified as leiomyoma due to strong immunopositivity for smooth muscle actin and desmin, being negative for CD34 and CD117. Two different gastric GIST lesions as well as the oesophageal leiomyoma and normal tissue were analysed for activating mutations in common hot spots of KIT (exon 9 and 11) and platelet-derived growth factor receptor alpha (exon 18), but in all probes wild-type sequences were found. These results are in accordance with the first published analyses of GIST lesions from Carney patients.

  1. A novel fully-human cytolytic fusion protein based on granzyme B shows in vitro cytotoxicity and ex vivo binding to solid tumors overexpressing the epidermal growth factor receptor.

    Science.gov (United States)

    Niesen, Judith; Hehmann-Titt, Grit; Woitok, Mira; Fendel, Rolf; Barth, Stefan; Fischer, Rainer; Stein, Christoph

    2016-05-01

    Human cytolytic fusion proteins (hCFPs) offer a promising immunotherapeutic approach for the treatment of solid tumors, avoiding the immunogenicity and undesirable side-effects caused by immunotoxins derived from plants or bacteria. The well-characterized human serine protease granzyme B has already been used as a therapeutic pro-apoptotic effector domain. We therefore developed a novel recombinant hCFP (GbR201K-scFv1711) consisting of an epidermal growth factor receptor-specific human antibody fragment and a granzyme B point mutant (R201K) that is insensitive to serpin B9 (PI9), a natural inhibitor of wild-type granzyme B that is often expressed in solid tumors. We found that GbR201K-scFv1711 selectively bound to epidermoid cancer and rhabdomyosarcoma cells and was rapidly internalized by them. Nanomolar concentrations of GbR201K-scFv1711 achieved the specific killing of epidermoid cancer cells by inducing apoptosis, and similar effects were observed in rhabdomyosarcoma cells when GbR201K-scFv1711 was combined with the endosomolytic substance chloroquine. The novel hCFP was stable in serum and bound to human rhabdomyosarcoma tissue ex vivo. These data confirm that GbR201K-scFv1711 is a promising therapeutic candidate suitable for further clinical investigation.

  2. Risk Factors of Diabetic Foot Ulcer and Clinical Significance of its Preventive Testing%糖尿病足溃疡的危险因素及预防性检查的临床意义

    Institute of Scientific and Technical Information of China (English)

    李鸿燕; 赵萍; 安欣儒

    2013-01-01

    Objective To investigate the risk factors and clinical values of preventive testing for diabetic foot ulcer, and to provide evidences for the clinical practice. Methods Fifty patients with diabetes mellitus hospitalized in the Affiliated Hospital of Hebei University from October 2009 to October 2012 were selected to serve as the research objects. They were divided into diabetic foot group and non - diabetic foot group (each n= 25). 20 physical examinees were simultaneously chosen as controls. The coagulation indexes, lower limb EMG and ultrasound results of all objects were observed. Results The diabetes patients' peripheral vascular disease, peripheral neuropathy and the changes of foot biomechanics were all risk factors for diabetic foot ulcers. The prothrombin time (PT) and activated partial thromboplastin time (APTT), fibrinogen (FIB) of diabetes patients were all significantly increased, there were statistically significant differences in above indicators between diabetes patients and healthy controls (P<0.05) as well as between diabetic foot group and non-diabetic foot group (P<0.05). The abnormal proportions of lower limb electromyography and ultrasound in diabetic foot patients were 84. 0% and 92.0% , respectively, which were both significantly higher than those of other two groups, and the differences were statistically significant (P<0.05). Conclusions Peripheral vascular disease, peripheral neuropathy and changes in foot biomechanics are risk factors for occurring foot ulcer in diabetic patients. The coagulation indexes, lower limb EMG and ultrasound results show clinical values in the diagnosis and treatment of diabetic foot ulcer, which can be used as the preventive examination items.%目的 分析糖尿病足溃疡的危险因素及预防性检查的临床情况,为临床工作提供参考依据.方法 选择河北大学附属医院2009年10月-2012年10月收治的糖尿病患者50例作为研究对象,其中糖尿病足及非糖尿病足者各25

  3. Clinical significance of serum vascular endothelial growth factor and b-fibroblast growth factor before and after chemotherapy in patients with small cell lung cancer%小细胞肺癌化疗前后血清VEGF和b-FGF的变化及临床意义

    Institute of Scientific and Technical Information of China (English)

    姜伟; 曹京燕; 潘博; 于雁

    2013-01-01

      目的:探讨化疗前后小细胞肺癌(SCLC)患者血清血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(b-FGF)水平的变化及临床意义。方法:用酶联免疫吸附法测定34例SCLC患者化疗前后VEGF和b-FGF的浓度变化,并与34例健康志愿者做对照。结果:化疗前肺癌组VEGF及b-FGF水平均明显高于健康对照组,差异有统计学意义(P0.05);Kaplan-Meier生存分析证实VEGF和b-FGF低浓度组的平均生存期均明显长于高浓度组(P0.05). No statistically significant relationship was found between the serum VEGF and the b-FGF levels. Considering the median con-centration of serum VEGF and b-FGF as a cut-off, the correlation analysis showed that the elevated VEGF level was correlated only with poor efficacy of chemotherapy and the increase in neuron-specific enolase (P0.05). Results from the Kaplan-Meier survival analysis showed that patients with low-level VEGF or b-FGF had significantly longer over-all survival time than those with high-level VEGF or b-FGF (P<0.01). Conclusion:Significant correlation was found between the survival of patients with SCLC and the serum VEGF or b-FGF level. Therefore, serum VEGF and b-FGF may be used as prognostic factors for survival and as indicators in predicting the efficacy of chemotherapy in patients with SCLC.

  4. Tomato Fruits Show Wide Phenomic Diversity but Fruit Developmental Genes Show Low Genomic Diversity.

    Science.gov (United States)

    Mohan, Vijee; Gupta, Soni; Thomas, Sherinmol; Mickey, Hanjabam; Charakana, Chaitanya; Chauhan, Vineeta Singh; Sharma, Kapil; Kumar, Rakesh; Tyagi, Kamal; Sarma, Supriya; Gupta, Suresh Kumar; Kilambi, Himabindu Vasuki; Nongmaithem, Sapana; Kumari, Alka; Gupta, Prateek; Sreelakshmi, Yellamaraju; Sharma, Rameshwar

    2016-01-01

    Domestication of tomato has resulted in large diversity in fruit phenotypes. An intensive phenotyping of 127 tomato accessions from 20 countries revealed extensive morphological diversity in fruit traits. The diversity in fruit traits clustered the accessions into nine classes and identified certain promising lines having desirable traits pertaining to total soluble salts (TSS), carotenoids, ripening index, weight and shape. Factor analysis of the morphometric data from Tomato Analyzer showed that the fruit shape is a complex trait shared by several factors. The 100% variance between round and flat fruit shapes was explained by one discriminant function having a canonical correlation of 0.874 by stepwise discriminant analysis. A set of 10 genes (ACS2, COP1, CYC-B, RIN, MSH2, NAC-NOR, PHOT1, PHYA, PHYB and PSY1) involved in various plant developmental processes were screened for SNP polymorphism by EcoTILLING. The genetic diversity in these genes revealed a total of 36 non-synonymous and 18 synonymous changes leading to the identification of 28 haplotypes. The average frequency of polymorphism across the genes was 0.038/Kb. Significant negative Tajima'D statistic in two of the genes, ACS2 and PHOT1 indicated the presence of rare alleles in low frequency. Our study indicates that while there is low polymorphic diversity in the genes regulating plant development, the population shows wider phenotype diversity. Nonetheless, morphological and genetic diversity of the present collection can be further exploited as potential resources in future.

  5. Planning a Successful Tech Show

    Science.gov (United States)

    Nikirk, Martin

    2011-01-01

    Tech shows are a great way to introduce prospective students, parents, and local business and industry to a technology and engineering or career and technical education program. In addition to showcasing instructional programs, a tech show allows students to demonstrate their professionalism and skills, practice public presentations, and interact…

  6. Expression and its significance of embryonic stem cell-associated factor Nanog in human gliomas%胚胎干细胞关键因子Nanog在人胶质瘤中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    郭雨霁; 邴鲁军; 刘尚明; 郝爱军

    2013-01-01

    Objective To explore the expression of embryonic stem cell-associated factor Nanog in human gliomas and its significance. Methods Forty cases of human gliomas were examined. The expression of Nanog was analysed by immunohistochemistry, reverse transcription polymerase chain reaction and Western blotting. Double immunofluorescence staining was used to investigate the coexpression of Nanog and markers of cancer stem cells or embryonic stem cell pluripotency-related gene Oct4. Results We found a positive correlation between the expression levels of Nanog and tumour malignancy. Immunohistochemistry showed that Nanogexpressed in both the nuclei and the cytoplasm of glioma cells. Double immunofluorescence staining revealed that more than 50% of Nanog-positive cells coexpressed the putative CSC markers CD133 and Nestin. More than 95% Nanog positive cells also expressed embryonic stem cell pluripotency-related gene Oct4. Conclusion The result suggests that Nanog is mainly expressed in cancer stem cells in gliomas and positively correlated with the malignancy of gliomas. Nanog may play an important role in the progress of gliomas.%目的 探讨胚胎干细胞关键因子Nanog在人胶质瘤中的表达及意义.方法 通过免疫荧光双标技术,分析40例胶质瘤组织内胚胎干细胞关键因子Nanog与肿瘤干细胞相关基因Nestin、CD133及胚胎干细胞全能性相关基因Oct4的共表达情况,并通过RT-PCR、Western blotting技术分析胶质瘤组织内Nanog与脑胶质瘤恶性程度之间的关系.结果 Nanog在胶质瘤组织中的表达随着胶质瘤病理级别增高而升高,而且超过50%的Nanog阳性细胞同时表达Nestin和CD133.95%以上的Nanog阳性细胞都同时表达胚胎干细胞全能性相关基因Oct4.结论 胶质瘤组织中Nanog多表达在肿瘤干细胞中,与胶质瘤的恶性程度呈正相关,在胶质瘤的发生、发展过程中起着重要作用,为研究胶质瘤的起源及胶质瘤的诊断和预后判断提供帮助.

  7. 血清FGF-21标记物在线粒体疾病的应用价值及临床意义%Diagnostic Value and Clinical Significance of Fibroblast Growth Factor 2 1 in Mitochondrial Disease

    Institute of Scientific and Technical Information of China (English)

    谷伟; 杨继雪; 崔玉环; 赵宝民

    2014-01-01

    Objective To investigate the value of serum fibroblast growth factor21(FGF-21)as an early diagnostic marker for mitochondrial disease.Methods Serum FGF-2 1 ,lactate and crea-tine kinase levels and lactate to pyruvate(L/P)ratio were determined in 16 patients with mito-chondrial disease,31 patients without mitochondrial disease and 41 normal subjects between Sep-tember 2009 and October 2013 to confirm FGF-21 as an effective and reliable biomarker for mito-chondrial disease.The sensitivity,odds ratio(OR)and reliability of FGF-21 for diagnosing mito-chondrial disease were analyzed.Results Compared with patients without mitochondrial disease or normal subj ects,serum FGF-2 1 concentrations significantly increased in patients with mito-chondrial disease.Furthermore,FGF-2 1 showed a markedly higher diagnostic OR than other bio-markers(45.7,P<0.000 1).The sensitivity and receiver operating characteristic curve analysis showed that serum FGF-2 1 was the best predictor of mitochondrial disease.After control for po-tential confounders,multivariate logistic regression analysis showed that only serum FGF-2 1 could predict and assess mitochondrial disease.Conclusion Serum FGF-2 1 is the most sensitive marker for diagnosing mitochondrial disease.Compared with lactate,creatine kinase and L/P rati-o,serum FGF-2 1 is the best predictor of mitochondrial disease.%目的:探讨血清 FGF-21标记物对线粒体疾病早期诊断价值。方法收集2009年9月至2013年10月间16例线粒体疾病患者,31例非线粒体疾病患者(疾病对照组),41例正常成人(正常对照组),比较血清 FGF-21、乳酸盐、肌酸激酶及乳酸盐丙酮酸盐,明确血清 FGF-21是诊断线粒体疾病有效及可靠性的生物标记物,通过统计学分析血清 FGF-21标记物诊断线粒体疾病的敏感性、优势比及可靠性。结果在线粒体疾病血清 FGF-21浓度明显增高,所有实验组血清 FGF-21明显表达不同,与其他生

  8. Expressions and clinical significances of nuclear factor-κB and hypoxia inducible factor-1α in prostate cancer%核转录因子-κB和缺氧诱导因子-1α在前列腺癌中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    李星; 何朝宏; 许长宝; 李云鹏

    2013-01-01

    目的:研究核转录因子-κB (nuclear factor kappa B,NF-κB)和缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)在前列腺癌中的表达及其临床意义.方法:采用逆转录聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫组织化学光谱纯试剂(spectrum pure,SP)法检测62例前列腺癌组织及17例良性前列腺增生组织中NF-κB和HIF-1α的mRNA和蛋白的表达.结果:(1)RT-PCR结果显示NF-κB和HIF-1α的mRNA在前列腺癌组中的相对表达量明显高于前列腺增生(P<0.05).免疫组织化学结果显示NF-κB和HIF-1α蛋白在前列腺癌组中的阳性表达率分别为62.90%和66.13%,明显高于前列腺增生组11.77%和5.88%(P<0.05).(2)NF-κB和HIF-1α的阳性表达均与前列腺癌的Gleason分级、临床分期有关(P<0.05),而与年龄无关(P>0.05).(3)NF-κB和HIF-1α的表达呈正相关(rs=0.368,P<0.05).结论:NF-κB和HIF-1α可能与前列腺癌的发生、发展密切相关,并可能在前列腺癌的浸润和转移中起重要作用.%Objective:To study expressions and clinical significances of nuclear factor-κB (NF-κB) and hypoxia inducible factor-1α (HIF-1α) in prostate cancer.Methods:RT-PCR and immunohistochemistry SP method were used to detect NF-κB and HIF-1α mRNA and protein expressions in 62 cases of prostate cancer tissues and 17 cases of prostatic hyperplasia tissues.Results:(1)RT-PCR results showed that NF-κB and HIF-1α mRNA expression levels were relatively significantly higher in prostate cancer group than in prostatic hyperplasia group,with statistical differences(P<0.05).Immunohistochemical results showed that positive expression rates of NF-κB and HIF-1α proteins in prostate cancer group were 62.90% and 66.13%,8.33% and 5.88%,significantly higher than those in prostatic hyperplasia group with statistically significant differences (P<0.05).(2) Expressions of NF-κB and HIF-1 α were correlated with Gleason grade

  9. 解剖学因素在退变性腰椎滑脱发生中的意义%The significance of the anatomical factors in the occurrence of degenerative lumbar spondylolisthesis

    Institute of Scientific and Technical Information of China (English)

    张剑刚; 丁文元; 申勇; 徐佳欣; 安志辉; 杨少坤

    2012-01-01

    Objectives: To analyzed the risk factors of degenerative lumbar spondylolisthesis(DLS). Methods: Retrospectively reviewed 60 cases of DLS patients as the case group, from July 2009 to September 2010. Male 20 cases, female 40 cases, age 51-75 years old, averaged 65.3±7.8 years old. Enrolled 60 cases age-and Bex-matched volunteers without DLS as the control group. The lumber lordosis angle(LLA), sacral horizontal angle(SHA), lumbar index(LI), disc angle(DA), disc height(DH) were measured on the lateral radiograph film and the length of transverse process of L5(TPL), the width of transverse process of L5(TPW) were measured on the anterior-posterior radiograph film. The LI, DA, DH of the control group were the parameter of vertebral and disc, which were corresponding to the slippage level of the case group. Examined the differences of these variables between groups and the association between DIS and these variables. Results: LLA, SHA. LI, DA, DH of the case group were 45.83°±10.42°, 28.35°±11.16°, 0.85±0.13, 7.24°±3.83°, 9.12± 2.73mm, and the control group were 47.48°±10.75°, 23.16°±10.68°, 0.96±0.10, 9.68°±5.54°, 10.54±2.48mm. SHA, LI, DA, DH had significant differences between groups(P<0.05). TPL, TPW of the case group were 2.15±0.43cm, 1.64±0.41cm, and TPL, TPW of the control group were 2.06±0.39cm, 1.57±0.32cm. There was no significant difference between groups(P>0.05). Logistic regression analysis showed statistically significant associations between DLS and LI, DA. The regression coefficients of LI and DA were -1.693,-1.406. Conclusions: DLS is significantly correlated with LI and DA. The decline of LI and DA may be the risk factors of DIS.%目的:探讨退变性腰椎滑脱(degenerative lumbar spondylolisthesis,DlS)的解剖学危险因素.方法:以2009年7月-2010年9月在我院确诊的60例DLS患者为观察对象(滑脱组),男20例,女40例,年龄51~75岁,平均65.3±7.8岁,L4滑脱42例,L5滑脱18例.

  10. Risk Aversion in Game Shows

    DEFF Research Database (Denmark)

    2008-01-01

    We review the use of behavior from television game shows to infer risk attitudes. These shows provide evidence when contestants are making decisions over very large stakes, and in a replicated, structured way. Inferences are generally confounded by the subjective assessment of skill in some games......, and the dynamic nature of the task in most games. We consider the game shows Card Sharks, Jeopardy!, Lingo, and finally Deal Or No Deal. We provide a detailed case study of the analyses of Deal Or No Deal, since it is suitable for inference about risk attitudes and has attracted considerable attention....

  11. Measuring performance at trade shows

    DEFF Research Database (Denmark)

    Hansen, Kåre

    2004-01-01

    Trade shows is an increasingly important marketing activity to many companies, but current measures of trade show performance do not adequately capture dimensions important to exhibitors. Based on the marketing literature's outcome and behavior-based control system taxonomy, a model is built...... that captures a outcome-based sales dimension and four behavior-based dimensions (i.e. information-gathering, relationship building, image building, and motivation activities). A 16-item instrument is developed for assessing exhibitors perceptions of their trade show performance. The paper presents evidence...

  12. Different histopathological subtypes of Hodgkin lymphoma show significantly different levels of FDG uptake

    DEFF Research Database (Denmark)

    Loft, Annika; Hutchings, Martin; Jakobsen, Annika Loft;

    2006-01-01

    HL underwent staging FDG-PET/CT after lymph node biopsy. Maximum SUV in each patient (SUV(max/total)) and in each affected region or organ (SUV(max)) were recorded. Mean SUV(max/total) was 9.3 g/ml in seven nodular lymphocyte predominance (NLP) patients, 16.3 g/ml in 38 nodular sclerosis (NS......) patients, 20.8 g/ml in 11 mixed cellularity (MC) patients, and 19.5 g/ml in four patients with unclassified classical HL (CHL-NOS), (ANOVA, p = 0.011). Out of 780 sites (600 lymph node regions plus 180 organs), 208 sites were found to be affected with HL. Mean SUV(max) was 8.3 g/ml in the 12 sites with NLP...

  13. Small Molecules Showing Significant Protection of Mice against Botulinum Neurotoxin Serotype A

    Science.gov (United States)

    2010-04-13

    phenyl-1H-indole-6- car - bonyl)-2-(4-hydroxyphenyl)thiophen-3-yl)-N-hydroxyacetamide (F4H). To a stirred solution of 3x (34 mg, 0.049 mmol) in THF/ MeOH...indole-6- car - bonyl)-2-(3-aminophenyl)thiophen-3-yl)-N-hydroxyacetamide (F3A). To a stirred solution of 3y (15 mg, 0.022 mmol) in THF/ MeOH (0.5 mL...Application of the multimolecule and multiconformational resp methodology to biopolymers : charge derivation for DNA, RNA, and proteins. J Comput Chem

  14. Big data integration shows Australian bush-fire frequency is increasing significantly

    Science.gov (United States)

    Dutta, Ritaban; Das, Aruneema; Aryal, Jagannath

    2016-01-01

    Increasing Australian bush-fire frequencies over the last decade has indicated a major climatic change in coming future. Understanding such climatic change for Australian bush-fire is limited and there is an urgent need of scientific research, which is capable enough to contribute to Australian society. Frequency of bush-fire carries information on spatial, temporal and climatic aspects of bush-fire events and provides contextual information to model various climate data for accurately predicting future bush-fire hot spots. In this study, we develop an ensemble method based on a two-layered machine learning model to establish relationship between fire incidence and climatic data. In a 336 week data trial, we demonstrate that the model provides highly accurate bush-fire incidence hot-spot estimation (91% global accuracy) from the weekly climatic surfaces. Our analysis also indicates that Australian weekly bush-fire frequencies increased by 40% over the last 5 years, particularly during summer months, implicating a serious climatic shift. PMID:26998312

  15. Tokyo Motor Show 2003; Tokyo Motor Show 2003

    Energy Technology Data Exchange (ETDEWEB)

    Joly, E.

    2004-01-01

    The text which follows present the different techniques exposed during the 37. Tokyo Motor Show. The report points out the great tendencies of developments of the Japanese automobile industry. The hybrid electric-powered vehicles or those equipped with fuel cells have been highlighted by the Japanese manufacturers which allow considerable budgets in the research of less polluting vehicles. The exposed models, although being all different according to the manufacturer, use always a hybrid system: fuel cell/battery. The manufacturers have stressed too on the intelligent systems for navigation and safety as well as on the design and comfort. (O.M.)

  16. Risk Aversion in Game Shows

    DEFF Research Database (Denmark)

    Andersen, Steffen; Lau, Morten I.; Rutström, E. Elisabet

    2008-01-01

    , and the dynamic nature of the task in most games. We consider the game shows Card Sharks, Jeopardy!, Lingo, and finally Deal Or No Deal. We provide a detailed case study of the analyses of Deal Or No Deal, since it is suitable for inference about risk attitudes and has attracted considerable attention....

  17. Phyllodes tumor showing intraductal growth.

    Science.gov (United States)

    Makidono, Akari; Tsunoda, Hiroko; Mori, Miki; Yagata, Hiroshi; Onoda, Yui; Kikuchi, Mari; Nozaki, Taiki; Saida, Yukihisa; Nakamura, Seigo; Suzuki, Koyu

    2013-07-01

    Phyllodes tumor of the breast is a rare fibroepithelial lesion and particularly uncommon in adolescent girls. It is thought to arise from the periductal rather than intralobular stroma. Usually, it is seen as a well-defined mass. Phyllodes tumor showing intraductal growth is extremely rare. Here we report a girl who has a phyllodes tumor with intraductal growth.

  18. Pembrolizumab Shows Promise for NSCLC.

    Science.gov (United States)

    2015-06-01

    Data from the KEYNOTE-001 trial show that pembrolizumab improves clinical outcomes for patients with advanced non-small cell lung cancer, and is well tolerated. PD-L1 expression in at least 50% of tumor cells correlated with improved efficacy.

  19. Create a Polarized Light Show.

    Science.gov (United States)

    Conrad, William H.

    1992-01-01

    Presents a lesson that introduces students to polarized light using a problem-solving approach. After illustrating the concept using a slinky and poster board with a vertical slot, students solve the problem of creating a polarized light show using Polya's problem-solving methods. (MDH)

  20. Reality show: um paradoxo nietzschiano

    Directory of Open Access Journals (Sweden)

    Ilana Feldman

    2011-01-01

    Full Text Available

    O fenômeno dos reality shows - e a subseqüente relação entre imagem e verdade - assenta-se sobre uma série de paradoxos. Tais paradoxos podem ser compreendidos à luz do pensamento do filósofo alemão Friedrich Nietzsche, que, através dos usos de formulações paradoxais, concebia a realidade como um mundo de pura aparência e a verdade como um acréscimo ficcional, como um efeito. A ficção é então tomada, na filosofia de Nietzsche, não em seu aspecto falsificante e desrealizador - como sempre pleiteou nossa tradição metafísica -, mas como condição necessária para que certa espécie de invenção possa operar como verdade. Sendo assim, a própria expressão reality show, através de sua formulação paradoxal, engendra explicitamente um mundo de pura aparência, em que a verdade, a parte reality da proposição, é da ordem do suplemento, daquilo que se acrescenta ficcionalmente - como um adjetivo - a show. O ornamento, nesse caso, passa a ocupar o lugar central, apontando para o efeito produzido: o efeito-de-verdade. Seguindo, então, o pensamento nietzschiano e sua atualização na contemporaneidade, investigaremos de que forma os televisivos “shows de realidade” operam paradoxalmente, em consonância com nossas paradoxais práticas culturais.

  1. Picasso on Show in Shanghai

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    A staff member of the National Picasso Museum of France checks one of the great Spanish artist Pablo Picasso’s works at the China Pavilion inside the site of the 2010 World Expo in Shanghai on October 12.Sixty-two priceless paintings and statues selected from the works of the renowned artist have been brought to the pavilion for an upcoming exhibition to premiere on October 18.Besides these representative masterpieces,50 valuable photographs showing the artist’s whole life will also be presented.The exhibition’s estimated value is 678 million euros ($934 million).It will be held until January 10,2012.

  2. Long-term omeprazole and esomeprazole treatment does not significantly increase gastric epithelial cell proliferation and epithelial growth factor receptor expression and has no effect on apoptosis and p53 expression

    Institute of Scientific and Technical Information of China (English)

    Istvan Hritz; Laszlo Herszenyi; Bela Molnar; Zsolt Tulassay; Laszlo Pronai

    2005-01-01

    AIM: To study the effect of proton pump inhibitor (PPI)treatment on patients with reflux esophagitis and its in vivo effect on apoptosis, p53- and epidermal growth factor receptor (EGFR) expression.METHODS: After informed consent was obtained, gastric biopsies of the antrum were taken from patients with reflux oesophagitis prior to and after 6 mo of 20 mg omeprazole (n = 14) or 40 mg esomeprazole (n = 12) therapy.Patients did not take any other medications known to affect the gastric mucosa. All patients were Helicobacter pylori negative as confirmed by rapid urease test and histology,respectively. Cell proliferation, apoptosis, EGFR, and p53expression were measured by immunohistochemical techniques. At least 600 glandular epithelial cells were encountered and results were expressed as percentage of total cells counted. Was considered statistically significant.RESULTS: Although there was a trend towards increase of cell proliferation and EGFR expression both in omeprazole and esomeprazole treated group, the difference was not statistically significant. Neither apoptosis nor p53 expression was affected.CONCLUSION: Long-term PPI treatment does not significantly increase gastric epithelial cell proliferation and EGFR expression and has no effect on apoptosis and p53 expression.

  3. Reality shows: uma abordagem psicossocial

    Directory of Open Access Journals (Sweden)

    Marília Pereira Bueno Millan

    Full Text Available Desde os primórdios da civilização, o ser humano mostra necessidade de representar cenicamente seus dramas pessoais e vicissitudes existenciais. O "reality show" é uma das versões pós-modernas da encenação da vida humana. Este artigo, por meio de uma pesquisa bibliográfica, analisa criticamente as relações existentes entre o "reality show" e aspectos psicossociais do comportamento humano. Conclui-se que tais programas televisivos são o retrato da contemporaneidade, ou seja, revelam a morte do sujeito, a fugacidade das experiências vividas, a desvalorização da história e o culto à imagem e à superficialidade. Por meio da sedução do espectador, mobilizam-se aspectos primitivos de seu psiquismo, fazendo com que ele se sinta narcisicamente poderoso e onipotente e se acredite dono do destino dos participantes do programa. Sugerem-se novos estudos que contribuam para a reflexão crítica e maior conscientização.

  4. "Medicine show." Alice in Doctorland.

    Science.gov (United States)

    1987-01-01

    This is an excerpt from the script of a 1939 play provided to the Institute of Social Medicine and Community Health by the Library of Congress Federal Theater Project Collection at George Mason University Library, Fairfax, Virginia, pages 2-1-8 thru 2-1-14. The Federal Theatre Project (FTP) was part of the New Deal program for the arts 1935-1939. Funded by the Works Progress Administration (WPA) its goal was to employ theater professionals from the relief rolls. A number of FTP plays deal with aspects of medicine and public health. Pageants, puppet shows and documentary plays celebrated progress in medical science while examining social controversies in medical services and the public health movement. "Medicine Show" sharply contrasts technological wonders with social backwardness. The play was rehearsed by the FTP but never opened because funding ended. A revised version ran on Broadway in 1940. The preceding comments are adapted from an excellent, well-illustrated review of five of these plays by Barabara Melosh: "The New Deal's Federal Theatre Project," Medical Heritage, Vol. 2, No. 1 (Jan/Feb 1986), pp. 36-47.

  5. Graphene Oxides Show Angiogenic Properties.

    Science.gov (United States)

    Mukherjee, Sudip; Sriram, Pavithra; Barui, Ayan Kumar; Nethi, Susheel Kumar; Veeriah, Vimal; Chatterjee, Suvro; Suresh, Kattimuttathu Ittara; Patra, Chitta Ranjan

    2015-08-05

    Angiogenesis, a process resulting in the formation of new capillaries from the pre-existing vasculature plays vital role for the development of therapeutic approaches for cancer, atherosclerosis, wound healing, and cardiovascular diseases. In this report, the synthesis, characterization, and angiogenic properties of graphene oxide (GO) and reduced graphene oxide (rGO) have been demonstrated, observed through several in vitro and in vivo angiogenesis assays. The results here demonstrate that the intracellular formation of reactive oxygen species and reactive nitrogen species as well as activation of phospho-eNOS and phospho-Akt might be the plausible mechanisms for GO and rGO induced angiogenesis. The results altogether suggest the possibilities for the development of alternative angiogenic therapeutic approach for the treatment of cardiovascular related diseases where angiogenesis plays a significant role.

  6. Casimir experiments showing saturation effects

    CERN Document Server

    Sernelius, Bo E

    2009-01-01

    We address several different Casimir experiments where theory and experiment disagree. First out is the classical Casimir force measurement between two metal half spaces; here both in the form of the torsion pendulum experiment by Lamoreaux and in the form of the Casimir pressure measurement between a gold sphere and a gold plate as performed by Decca et al.; theory predicts a large negative thermal correction, absent in the high precision experiments. The third experiment is the measurement of the Casimir force between a metal plate and a laser irradiated semiconductor membrane as performed by Chen et al.; the change in force with laser intensity is larger than predicted by theory. The fourth experiment is the measurement of the Casimir force between an atom and a wall in the form of the measurement by Obrecht et al. of the change in oscillation frequency of a 87 Rb Bose-Einstein condensate trapped to a fused silica wall; the change is smaller than predicted by theory. We show that saturation effects can exp...

  7. Anthropological significance of phenylketonuria.

    Science.gov (United States)

    Saugstad, L F

    1975-01-01

    The highest incidence rates of phenylketonuria (PKU) have been observed in Ireland and Scotlant. Parents heterozygous for PKU in Norway differ significantly from the general population in the Rhesus, Kell and PGM systems. The parents investigated showed an excess of Rh negative, Kell plus and PGM type 1 individuals, which makes them similar to the present populations in Ireland and Scotlant. It is postulated that the heterozygotes for PKU in Norway are descended from a completely assimilated sub-population of Celtic origin, who came or were brought here, 1ooo years ago. Bronze objects of Western European (Scottish, Irish) origin, found in Viking graves widely distributed in Norway, have been taken as evidence of Vikings returning with loot (including a number of Celts) from Western Viking settlements. The continuity of residence since the Viking age in most habitable parts of Norway, and what seems to be a nearly complete regional relationship between the sites where Viking graves contain western imported objects and the birthplaces of grandparents of PKUs identified in Norway, lend further support to the hypothesis that the heterozygotes for PKU in Norway are descended from a completely assimilated subpopulation. The remarkable resemblance between Iceland and Ireland, in respect of several genetic markers (including the Rhesus, PGM and Kell systems), is considered to be an expression of a similar proportion of people of Celtic origin in each of the two countries. Their identical, high incidence rates of PKU are regarded as further evidence of this. The significant decline in the incidence of PKU when one passes from Ireland, Scotland and Iceland, to Denmark and on to Norway and Sweden, is therefore explained as being related to a reduction in the proportion of inhabitants of Celtic extraction in the respective populations.

  8. Natural stochasticity vs. management effort: use of year-to-year variance for disentangling significance of two mutually confounding factors affecting water quality of a Norwegian cold dimictic lake

    Directory of Open Access Journals (Sweden)

    A. T. Romarheim

    2014-11-01

    Full Text Available Natural stochasticity can pose challenges in managing the quality of the environment, or hinder understanding of the system structure. It is problematic because unfavourable stochastic event cancels the costly management effort and because favourable stochastic event overestimates success of the management effort. This paper presents a variance-based modelling method that can be used to quantify the extent to which the natural stochasticity can affect the target environment. We use a case study of a lake water quality assessment in a Norwegian lake of Årungen, together with a lake model MyLake, in order to present the method, and how this method could assist in answering scientific and managerial questions. Specifically, the case study's goal was to disentangle the respective significance of nutrient loading (management and weather (the confounding natural stochasticity. Many scientifically and managerially relevant understandings have been revealed. For example, variation in runoff volume was most prevalent during autumn and winter, while variation in phosphorus inflow was most extensive from late winter to early spring. Thermal related properties in the lake were mostly determined by weather conditions, whereas loading was the most important factor for phytoplankton biomass and water transparency. Mild winters and greater inputs of suspended matter and phosphorus were followed by increased phytoplankton biomass and light attenuation. These findings suggest also that future changes in the global climate may have important implications for local water management decision-making. The present method of disentangling mutually confounding factors is not limited to lake water quality studies and therefore should provide certain utility in other application field of modelling.

  9. 腹膜透析清除钠的相关因素分析及其临床意义%Factors relating to sodium clearance in peritoneal dialysis and their clinical significance

    Institute of Scientific and Technical Information of China (English)

    单亦升; 丁小强; 吉俊

    2011-01-01

    Objective To explore the factors relating to sodium clearance in peritoneal dialysis patients.Methods A total of 156 maintenance peritoneal dialysis patients who had completed the modified peritoneal equilibrium test (PET) and dialysis adequacy test were enrolled in this study.Multifactor regression analysis was used for data analysis.Results This study firstly disclosed the influence factors relating to the peritoneal sodium removal,including infusion volume and ultrafiltration every day, sodium dip during modified PET, and sodium concentration difference between serum and flesh dialysate.The influence factors relating to total sodium clearance included ultrafiltration every day, urine volume every day, sodium dip during modified PET, and sodium concentration difference between serum and fresh dialysate.Multifactor regression analysis indicated the presence of significant relationships.Conclusions (1) Sufficient fluid removal is necessary for adequate sodium removal when traditional dialysate is used; (2) Low sodium dialysate may be useful for the relief of sodium retention; (3) Index of sodium clearance should be included in the evaluation of dialysis adequacy.%目的 揭示腹膜透析患者钠清除的相关因素.方法 156例维持性腹膜透析患者,行改良腹膜平衡试验和透析充分性检查,对结果 做多元线性回归分析.结果 揭示了腹膜对钠清除的影响因素有透析剂量、每日净超滤量、血钠与新鲜透析液钠浓度差值和钠沉降率,总钠清除的影响因素有每日净超滤量、每日尿量、血钠与新鲜透析液钠浓度差值和钠沉降率.多元线性回归分析显示有显著相关性(P<0.01).结论 使用传统的腹膜透析液和足够的液体清除量是保证足够的钠清除量的最重要条件;使用低钠透析液应该是解决钠潴留的有效办法;评价透析充分性应包含钠清除的指标.

  10. In Vivo Imaging Reveals Significant Tumor Vascular Dysfunction and Increased Tumor Hypoxia-Inducible Factor-1α Expression Induced by High Single-Dose Irradiation in a Pancreatic Tumor Model.

    Science.gov (United States)

    Maeda, Azusa; Chen, Yonghong; Bu, Jiachuan; Mujcic, Hilda; Wouters, Bradly G; DaCosta, Ralph S

    2017-01-01

    To investigate the effect of high-dose irradiation on pancreatic tumor vasculature and microenvironment using in vivo imaging techniques. A BxPC3 pancreatic tumor xenograft was established in a dorsal skinfold window chamber model and a subcutaneous hind leg model. Tumors were irradiated with a single dose of 4, 12, or 24 Gy. The dorsal skinfold window chamber model was used to assess tumor response, vascular function and permeability, platelet and leukocyte adhesion to the vascular endothelium, and tumor hypoxia for up to 14 days after 24-Gy irradiation. The hind leg model was used to monitor tumor size, hypoxia, and vascularity for up to 65 days after 24-Gy irradiation. Tumors were assessed histologically to validate in vivo observations. In vivo fluorescence imaging revealed temporary vascular dysfunction in tumors irradiated with a single dose of 4 to 24 Gy, but most significantly with a single dose of 24 Gy. Vascular functional recovery was observed by 14 days after irradiation in a dose-dependent manner. Furthermore, irradiation with 24 Gy caused platelet and leukocyte adhesion to the vascular endothelium within hours to days after irradiation. Vascular permeability was significantly higher in irradiated tumors compared with nonirradiated controls 14 days after irradiation. This observation corresponded with increased expression of hypoxia-inducible factor-1α in irradiated tumors. In the hind leg model, irradiation with a single dose of 24 Gy led to tumor growth delay, followed by tumor regrowth. Irradiation of the BxPC3 tumors with a single dose of 24 Gy caused transient vascular dysfunction and increased expression of hypoxia-inducible factor-1α. Such biological changes may impact tumor response to high single-dose and hypofractionated irradiation, and further investigations are needed to better understand the clinical outcomes of stereotactic body radiation therapy. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Expression and significance of nerve growth factor in glioma and cerebrospinal fluid%神经生长因子在人脑胶质瘤及脑脊液中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    陆新宇; 陆培松; 李巧玉; 杨勇

    2012-01-01

    Objective: To study the expression and clinical significance of nerve growth factor( NGF ) in glioma and cerebral spinal fluid( CSF ). Methods: A total of 86 cases of glioma and CSF were determined for NGF expression by immunohistochemical method( Envision ) and ELISA. Ten cases of tissue samples from patients with traumatic brain injury who had received decompression surgery and 10 cases of CSF samples from patients underwent spinal anesthesia without nervous system disease were taken as the corresponding control groups. Results: In glioma and CSF cases, the NGF expression levels was higher than that in corresponding two control groups, the difference was statistically significant( P <0. 05 ). There was significant difference in NGF expression between different grades of glioma( P <0.05 );the expression of NGF increased in parallel with the increase of pathological grade of glioma( P <0. 05 ); and there was also significant difference in glioma incidence between the supratentorial tumour and infratentorial tumour( P <0.05 ). Conclusion: NGF expression was a consistent feature of cerebral tumor, especially in glioma, and its expression was correlated with the genesis and development of glioma.%目的:研究神经生长因子(nerve growth factor,NGF)在脑星形胶质瘤组织及脑脊液中的表达特点,探讨其与肿瘤病理分级、实体肿瘤部位的关系,以及在肿瘤发生过程中的作用.方法:收集86例星形胶质瘤组织及术前脑脊液标本,取同期10例严重脑外伤行减压术患者的脑组织及10例无神经系统疾病史因其他原因而行腰麻手术患者的脑脊液分别作为对照组脑组织和对照组脑脊液.通过免疫组织化学染色观察NGF在胶质瘤细胞及对照组脑组织细胞中的表达,并采用ELISA法检测NGF在胶质瘤患者及对照组脑组织和脑脊液中的表达水平.结果:胶质瘤组织及脑脊液中NGF表达水平明显高于对照组(P<0.05),高级别胶质瘤中NGF表达

  12. Significant Scales in Community Structure

    CERN Document Server

    Traag, V A; Van Dooren, P

    2013-01-01

    Many complex networks show signs of modular structure, uncovered by community detection. Although many methods succeed in revealing various partitions, it remains difficult to detect at what scale some partition is significant. This problem shows foremost in multi-resolution methods. We here introduce an efficient method for scanning for resolutions in one such method. Additionally, we introduce the notion of "significance" of a partition, based on subgraph probabilities. Significance is independent of the exact method used, so could also be applied in other methods, and can be interpreted as the gain in encoding a graph by making use of a partition. Using significance, we can determine "good" resolution parameters, which we demonstrate on benchmark networks. Moreover, optimizing significance itself also shows excellent performance. We demonstrate our method on voting data from the European Parliament. Our analysis suggests the European Parliament has become increasingly ideologically divided and that nationa...

  13. Influencing Factors and Significance of Portal Hypertensive Gastropathy in Liver Cirrhosis%影响门静脉高压性胃病的多因素回归分析

    Institute of Scientific and Technical Information of China (English)

    赵智; 罗俊卿; 刘小利; 段泽星

    2015-01-01

    【目的】探讨肝硬化门脉高压性胃病(portal hypertensive gastropathy ,PHG)的影响因素及其意义。【方法】将158例肝硬化患者分为无 PHG 组、轻度 PHG 组与重度 PHG 组,比较各组终末期肝病模型(MELD)评分、血氨、血清白蛋白、腹水、食管静脉曲张程度、脾脏大小、门静脉内径、胃蛋白酶原比值(PGI/PGII ,PGR)、幽门螺杆菌(Hp)感染差异,随后进行多因素 Logistic 回归分析。【结果】不同组别肝硬化患者的 MELD 评分、血清白蛋白、食管静脉曲张程度、门静脉内径、PGR 比较差异具有统计学意义;多因素 Logistic 回归分析表明 MELD 评分、食管静脉曲张程度、门静脉内径、PGR 与 PHG 有较高相关度。【结论】肝硬化患者的 MELD 评分、食管静脉曲张程度、门静脉内径是 PHG 的危险因素,而 PGR 是 PHG 的保护因素,对于指导治疗及判断预后有一定帮助。%Objective] To explore the influencing factors and significance of portal hypertensive gastropathy (PHG) in liver cirrhosis .[Methods] A total of 158 cases of liver cirrhosis were divided into non‐PHG ,mild PHG and severe PHG groups .And the scores of model for end‐stage liver disease (MELD) were assessed .The profiles of blood ammonia ,serum albumin ,ascites ,esophageal varices ,spleen size ,diameter of main portal vein ,pepsino‐gen ratio (PGR) and HP infection were compared among three groups .Multivariate Logistic regression analyses were performed .[Results] The differences were statistically significant for MELD score ,serum albumin ,esopha‐geal varices ,diameter of main portal vein and PGR among three groups .Multivariate logistic regression analysis indicated that MELD score ,esophageal varices ,diameter of main portal vein and PGR were highly correlated with PHG .[Conclusion] MELD score ,esophageal varices and diameter of main portal vein are the risk factors .How‐ever ,PGR is a

  14. 文学接受环节中文本意义表达及非人性因素%The Expression of the Significance of Text and Impersonal Factor in the Process of Literary Reception

    Institute of Scientific and Technical Information of China (English)

    黄雅婷

    2016-01-01

    20世纪后,文学理论的研究呈现出多重结构的模式,文学性逐渐淡化,文学文本成为文学消费的产物,一个新的媒体和文本作者形成更密切的关系。伴随着研究上下文的转移,文学接受研究的联系也产生了新的变化。文本中的意义表达水平的表达式出现更多的非人为因素,新媒体和网络已成为文学生产和传播的新途径。%After twenty century the study of literary theory presents a pattern of multi structure literaturenity was gradually dilution literary text became the products of literary consumption a new media and text author forming closer ties. Accompanied by the study of the context of the transfer literature reception research link also produced a new change. Text in the expression of meaning significance expression level appears imper⁃sonal factor. New media and network has become a new way for the production and dissemination of litera⁃ture.

  15. ER、EGFR在分化型甲状腺癌中的表达及其临床意义%Expression and Clinical Significance of Estrogen Receptor and Epidermal Growth Factor Receptor in the Differentiated Thyroid Carcinoma

    Institute of Scientific and Technical Information of China (English)

    刘菊林; 吴跃龙; 李静; 卢国丰; 陈军

    2013-01-01

    Objective: To investigate the expression and significance of estrogen receptor (ER) and epidermal growth factor receptor (EGFR) in the differentiated thyroid carcinoma (DTC). Methods: The expression of ER and EGFR was detected in 45 cases of DTC with paraffin-embedded by S-P immunohistochemical technique. Results: The positive percentage of ER expression in DTC was 37.3 %, and it is significantly higher than that in thyroid adenoma and normal thyroid (P<0.05); The positive percentage of EGFR expression of was 57.7 % in DTC, and it is significantly higher than that in others (P<0.05). The expression of ER was associated with the gender and age, and the expression of EGFR was associated with lymph node metastasis. Conclusion: The expression of ER and EGFR may be served as markers in identifying thyroid cancer and thyroid innocuous node. EGFR positive expression is predictive of poor prognosis.%目的:探讨雌激素受体(ER)和表皮生长因子受体(EGFR)在分化型甲状腺癌(DTC)中的表达及其意义.方法:用免疫组化SP法检测45例(DTC)组织中ER和EGFR的表达.结果:ER在DTC组中的阳性率为37.3%,明显高于良性腺瘤和正常组(P<0.05);EGFR在DTC组中的阳性率为57.7%,明显高于两对照组(P<0.05).ER在DTC中的表达与性别和年龄有关;EGFR的表达与DTC中的淋巴结转移有关.结论:ER和EGFR的表达可以作为鉴别甲状腺肿瘤良恶性的指标EGFR可作为甲状腺癌患者预后的指标.

  16. 染矽尘小鼠早期肺组织bFGF与FGFR-1的表达及意义%Expression and Significance of Basic Fibroblast Growth Factor and Fibroblast Growth Factor Receptor-1 in Early Stage Lung Tissues of Silica Exposed Mice

    Institute of Scientific and Technical Information of China (English)

    李丹; 向军俭; 王宏; 陶俊; 邓宁; 钟雪云

    2011-01-01

    Objective To investigate the expression of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor-1 (FGPR-1) in early stage lung tissues of silica exposed mice and to probe into the significance of bFGF in occurrence of silicosis. Methods The mice were instilled with silicon dioxide through trachea to establish modeling (0.2 g/kg). Samples of lung tissue were collected at the 7 th, 14 th, 21 st and 28 th day after injection.RT-PCR was used to detect the gene transcription of bFGF. Immunohistochemistry was used to analyze the expression of bFGF and FGFR-1 in lung tissue from silicotic and control mice. Results The results of RT-PCR showed that the gray scales of bFGF gene transcription in the silicotic group were 32.08% ± 0.05 % ~ 49.07 % ±14.37%, and in the control group were 34.79% ± 15.50%. The immunohistochemistry result showed the protein expression of bFGF was mainly in vascular smooth muscle cell, macrophage and bronchial epithelial cells and the expression had no difference between the silicotic group and the control group at the 7th, 14th and 21st day after injection of silicon dioxide (P>0.05). Nodular lesions in the lungs began to express small mount of bFGF at the 28th day after injection. The expression of FGFR-1 was significantly elevated from the 7th day to 28th day (IOD:16157.63 ± 359.13 vs. 45 873. 24±359. 13~145 030.70±577.49,P<0.05). And it was expressed by vascular smooth muscle cell, macrophage and mesenchymal cell. Conclusions No increase in the expression of bFGF is seen at the early stage of silicosis in mice, but FGFR-1 is highly expressed in a variety of lung cells. The results show that bFGF might play a role in early infection of silicosis through highly expressed FGFR-1, or play no role in silicosis.%目的 观察染矽尘小鼠早期肺组织碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)及成纤维细胞生长因子受体-1(fibroblast growth factor receptor-1,FGFR-1

  17. The VITRO Score (Von Willebrand Factor Antigen/Thrombocyte Ratio as a New Marker for Clinically Significant Portal Hypertension in Comparison to Other Non-Invasive Parameters of Fibrosis Including ELF Test.

    Directory of Open Access Journals (Sweden)

    Stephanie Hametner

    Full Text Available Clinically significant portal hypertension (CSPH, defined as hepatic venous pressure gradient (HVPG ≥10 mmHg, causes major complications. HVPG is not always available, so a non-invasive tool to diagnose CSPH would be useful. VWF-Ag can be used to diagnose. Using the VITRO score (the VWF-Ag/platelet ratio instead of VWF-Ag itself improves the diagnostic accuracy of detecting cirrhosis/ fibrosis in HCV patients.This study tested the diagnostic accuracy of VITRO score detecting CSPH compared to HVPG measurement.All patients underwent HVPG testing and were categorised as CSPH or no CSPH. The following patient data were determined: CPS, D'Amico stage, VITRO score, APRI and transient elastography (TE.The analysis included 236 patients; 170 (72% were male, and the median age was 57.9 (35.2-76.3; 95% CI. Disease aetiology included ALD (39.4%, HCV (23.4%, NASH (12.3%, other (8.1% and unknown (11.9%. The CPS showed 140 patients (59.3% with CPS A; 56 (23.7% with CPS B; and 18 (7.6% with CPS C. 136 patients (57.6% had compensated and 100 (42.4% had decompensated cirrhosis; 83.9% had HVPG ≥10 mmHg. The VWF-Ag and the VITRO score increased significantly with worsening HVPG categories (P<0.0001. ROC analysis was performed for the detection of CSPH and showed AUC values of 0.92 for TE, 0.86 for VITRO score, 0.79 for VWF-Ag, 0.68 for ELF and 0.62 for APRI.The VITRO score is an easy way to diagnose CSPH independently of CPS in routine clinical work and may improve the management of patients with cirrhosis.

  18. Significant NRC Enforcement Actions

    Data.gov (United States)

    Nuclear Regulatory Commission — This dataset provides a list of Nuclear Regulartory Commission (NRC) issued significant enforcement actions. These actions, referred to as "escalated", are issued by...

  19. The absolute lymphocyte/monocyte ratio recovery during ABVD treatment cycles is not significantly impacted by the use of myeloid growth factors and predicts clinical outcomes in classical Hodgkin lymphoma regardless of their use

    Directory of Open Access Journals (Sweden)

    Kaufman GP

    2014-07-01

    Full Text Available Gregory P Kaufman,1 Kay M Ristow,1,2 Svetomir N Markovic,1,2 Luis F Porrata1,2 1Department of Internal Medicine, 2Division of Hematology, Mayo Clinic, Rochester, MN, USA Abstract: Risk stratification of patients with classical Hodgkin lymphoma (cHL remains suboptimal. The ratio of the absolute lymphocyte count (ALC to absolute monocyte count (AMC both at diagnosis and during subsequent recovery from serial cycles of chemotherapy predicts survival in cHL, and possesses advantages over other commonly used prognostic markers. Myeloid growth factors (MGFs, while not strongly recommended for use in adriamycin, bleomycin, vinblastine, and dacarbazine (ABVD treatment cycles, are not uncommonly used to prevent the negative consequences of neutropenia. The effect that MGFs have on the ALC/AMC ratio during ABVD treatment cycles, if any, remains unclear. We retrospectively evaluated 208 patients with cHL, who were diagnosed, treated, and followed at Mayo Clinic Rochester between 1990 and 2014, and who had quantifiable records for the use of MGFs during ABVD treatment cycles. Having an ALC/AMC ratio <1.1 during all treatment cycles was confirmed as being a negative predictor of overall and progression free survival (hazard ratio [HR] 0.06, 95% confidence interval [CI] 0.03–0.14 and HR 0.08, 95% CI 0.04–0.17, respectively. Data on both the ALC/AMC ratio and use of MGFs were available for 1,979 half treatment cycles. When stratified to whether or not MGFs were given, the change in the ALC/AMC ratio as compared to the prior half cycle was found to be statistically insignificant (P=0.3445. No survival advantage was found with the administration of MGFs in any cycle of therapy (log rank P=0.5713. Our data validate the prognostic significance of having an ALC/AMC ratio of ≥1.1 regardless of the use of MGFs. Keywords: myeloid growth factors, classical Hodgkin lymphoma, survival ALC/AMC ratio, ABVD chemotherapy

  20. 心理社会因素在应对重大突发疾病中的作用%The Role of Psychosocial Factors in the Significant Emergencies Disease Responding

    Institute of Scientific and Technical Information of China (English)

    傅义宁; 罗晓

    2011-01-01

    With the rapid development of human society and constantly changing spectrum of disease, medical model has been changed from a single biological model for the Biological-Social-Psychological Model. From the SARS pandemic in April 2002, a large debris flow occurred in Zhouqu County of Gansu Province in August 2010,psychosocial factors have being played a significant role on the bodys immune function in a potential approach. Playing a role in the psycho - social intervention system approach, regulating the bodys stress response, strengthening the immune system to respond to major emergencies diseases have become urgent issues. From the initial spontaneous psycho-social intervention system to play a role in the SARS period, the long-term emergency system and a network type of psycho-social intervention system based on the present, the role of psychosocial factors has become increasingly prominent in response to major sudden illness.%随着人类社会的快速发展和疾病谱的不断变化,医学模式已经从单一的生物模式转变为生物心理社会模式,从2002年4月我国SARS的大流行,到2010年8月甘肃舟曲巨大泥石流灾害的发生,心理社会因素已经以一种潜在的方式对人体的免疫功能发挥巨大作用.发挥心理-社会干预系统的作用.调节人体应激反应,增强免疫力来应对重大突发疾病已成为刻不容缓的课题.通过SARS期间自发式的心理一社会干预系统初步发挥作用,到目前建立长效的应急体系和心理-社会干预网络,心理社会因素在应对重大突发疾病中的作用日益突出.

  1. Choosing Outcomes of Significance.

    Science.gov (United States)

    Spady, William G.

    1994-01-01

    Outcomes are high-quality, culminating demonstrations of significant learning in context. The High Success Network uses the "Demonstration Mountain" to differentiate among three major "learning zones" and six different forms of learning demonstrations that increase in complexity, generalizability, and significance, along with…

  2. Expression and significance of leukemia inhibitory factor in placental tissues of patients with preeclampsia%白血病抑制因子在子痫前期患者胎盘组织中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    王海燕; 杨海澜; 马惠静; 冯敏; 索明莉

    2011-01-01

    目的:测定白血病抑制因子(LIF)在子痫前期患者胎盘组织中的表达变化,探讨其在子痫前期发病过程中的作用.方法:选取子痛前期(子痫前期轻度组和重度组)和正常妊娠孕妇作为研究组和对照组,采用免疫组化法测定其胎盘组织中LIF的表达,并通过BI2000医学图像分析系统对染色结果进行定量检测并作比较.结果:LIF在3组中均有表达,且主要表达于滋养细胞胞质中;子痛前期轻度组及重度组与正常妊娠组相比,胎盘组织中LIF的表达明显减弱,差异有统计学意义(P0.05).结论:LIF对正常妊娠的维持起到了一定的保护作用;LIF在胎盘组织中的低表达影响了滋养细胞的浸润能力,可能是子痛前期发病的病因之一.%Objective: To detect the changes of leukemia inhibitory factor (LIF) expression in placental tissues of patients with preeclampsia, explore its value in pathogenesis of preeclampsia. Methods: The women with preeclampsia and normal pregnant women were selected as study group ( mild preeclampsia group and severe preeclampsia group) and control group, respectively; immunohistochemical method was used to detect the expression levels of LIF in placental tissues of the two groups; BI2000 medical image analysis system was used for quantitative detection and comparison. Results: LIF expressed in the three groups, mostly locating in cytoplasma of trophoblast cells; compared with control group, the expression level of LIF in study group decreased significantly (P <0. 01 ); there was no significant difference in the expression level of LIF between mild precclampsia group and severe preeclampsia group ( P > 0. 05 ) . Conclusion: LIF may play a protective role in the maintenance of normal pregnancy; the low expression of LIF in placental tissues affects the invasive ability of trophoblast cells, which may be one of the pathogenesis of preeclampsia.

  3. 揭開日本學生傑出表現背後的秘密:教學研究 The Most Significant Factor Underlying Japanese Students’ Academic Success: Lesson Study

    Directory of Open Access Journals (Sweden)

    黃源河Yuanher Robin Hwang

    2011-12-01

    Full Text Available 1995年日本中小學生在國際數理競賽(Trends in International Mathematics and Science Study, TIMSS)傲人的表現,引發國際學者的好奇,紛紛探究其背後的原因。結果發現,日本教師專業發展模式──「教學研究」居功厥偉。雖然最近兩次國際數理競賽,日本學生的表現略微下滑,但其實施近百年,頗具特色的「教學研究」仍值得各國關注。本研究旨在探討日本的教學研究如何聚焦於學生學習,以增進教師專業成長。全文先介紹日本的課堂教學,其次說明其幕後推手「教學研究」,並舉出運作實例,接著進行學理分析,最後討論對我國師資培育與中小學教育的啟示。 The outstanding performance of Japanese students in the Trends in International Mathematics and Science Study (TIMSS in 1995 attracted significant international attention. Numerous education researchers have attempted to determine what factors or practices underlie Japanese students’ success. They found that lesson study, a specialized model of teacher professional development used in Japan for over 100 years, can be credited with significant contributions to the improvement of instructional practices, resulting in excellent student performance and academic achievement. This study investigates how lesson study furthers teacher professional development and improves student learning, and examines and analyzes the underlying rationale. Finally, we present the practical implications of this investigation and analysis to inform current discussions on education and teacher professional development in Taiwan.

  4. Restrictive Factors and Historical Significance of Rectification Movement in the Later Anti-Japanese War%抗战后期敌后整风的制约因素与历史意义

    Institute of Scientific and Technical Information of China (English)

    向伟

    2015-01-01

    抗战后期,中共在全党开展整风学习运动。处在敌后的各根据地,尽管也都在规定时间内完成整风,但从当时的情况来看,这些根据地的整风受到诸多因素的制约,如党员干部对整风的重要性缺乏认识、敌后环境异常艰苦、党员与干部知识水平较低以及整风脱离实际等。分析敌后整风情况,无论从认识还是实践上,对于如今的党建工作也有一定的启发意义。%In the latter part of the Anti-Japanese War, the Chinese Communists Party carried out the rectification movement. Although the rectification was completed in a short time, from the point of view of the situation at that time, the rectification movement was restricted by many factors , such as the lack of understanding of importance of Party members and cadres ,the difficult environment in the enemy’s rear area, low level of knowledge and rectification from the reality etc. Analysis of rectification situation both from behind enemy lines, understanding and practice, also have certain enlightening significance for today ’s rectification learning.

  5. Significantly Higher Peripheral Insulin-Like Growth Factor-1 Levels in Patients With Major Depressive Disorder or Bipolar Disorder Than in Healthy Controls: A Meta-Analysis and Review Under Guideline of PRISMA.

    Science.gov (United States)

    Tu, Kun-Yu; Wu, Ming-Kung; Chen, Yen-Wen; Lin, Pao-Yen; Wang, Hung-Yu; Wu, Ching-Kuan; Tseng, Ping-Tao

    2016-01-01

    An increasing amount of research has focused on insulin-like growth factor-1 (IGF-1) because of multiple neurotrophic effects, including neurogenesis, remyelination, and synaptogenesis. In addition, IGF-1 can mediate an antidepressant effect in patients with major affective disorder, and its levels in the cerebrospinal fluid have been found to vary with antidepressant treatment. Furthermore, it has been proven to crossover the blood-brain barrier, with a reciprocal feedback loop being the central effect. However, recent studies have reported inconclusive findings about the role of IGF-1 in major affective disorder. The aim of the current study was to conduct a thorough meta-analysis of changes in peripheral IGF-1 levels in patients with major depressive disorder (MDD) or bipolar disorder (BD). We conducted a thorough literature search and compared peripheral IGF-1 levels in patients with MDD or BD and in healthy controls, and investigated clinical variables through meta-regression. Electronic research was conducted through platform of PubMed. We used inclusion criteria as clinical trials discussing comparisons of peripheral IGF-1 protein levels in patients with MDD or BD and those in healthy controls. We analyzed the cases from 9 studies with the random-effect model. The main finding was that peripheral IGF-1 levels in the patients were significantly higher than in the healthy controls (P data with growth hormone in current studies are the main limitations of this meta-analysis. Our results indicated that peripheral IGF-1 levels may not be an indicator of disease severity, but may be a disease trait marker or an indicator of cognition. However, further investigations on the correlation between cognitive function and peripheral IGF-1 levels are needed to explore the role of IGF-1 in the pathophysiology of MDD and BD.

  6. Oxidative stress as a significant factor for development of an adaptive response in irradiated and nonirradiated human lymphocytes after inducing the bystander effect by low-dose X-radiation

    Energy Technology Data Exchange (ETDEWEB)

    Ermakov, Aleksei V., E-mail: avePlato@mail.ru [Research Centre for Medical Genetics, Russian Academy of Medical Science, ul. Moskvorechye, 1, Moscow 115478 (Russian Federation); Konkova, Marina S.; Kostyuk, Svetlana V.; Egolina, Natalya A.; Efremova, Liudmila V.; Veiko, Natalya N. [Research Centre for Medical Genetics, Russian Academy of Medical Science, ul. Moskvorechye, 1, Moscow 115478 (Russian Federation)

    2009-10-02

    X-radiation (10 cGy) was shown to induce in human lymphocytes transposition of homologous chromosomes loci from the membrane towards the centre of the nucleus and activation of the chromosomal nucleolus-forming regions (NFRs). These effects are transmitted by means of extracellular DNA (ecDNA) fragments to nonirradiated cells (the so-called bystander effect, BE). We demonstrated that in the development of the BE an important role is played by oxidative stress (which is brought about by low radiation doses and ecDNA fragments of the culture medium of the irradiated cells), by an enzyme of apoptosis called caspase-3, and by DNA-binding receptors of the bystander cells, presumably TLR9. Proposed herein is a scheme of the development of an adaptive response and the BE on exposure to radiation. Ionizing radiation induces apoptosis of the radiosensitive fraction of cells due to the development of the 'primary' oxidative stress (OS). DNA fragments of apoptotic cells are released into the intercellular space and interact with the DNA-binding receptors of the bystander cells. This interaction activates in lymphocytes signalling pathways associated with synthesis of the reactive oxygen species and nitrogen species, i.e., induces secondary oxidative stress accompanied by apoptosis of part of the cells, etc. Hence, single exposure to radiation may be followed by relatively long-lasting in the cellular population oxidative stress contributing to the development of an adaptive response. We thus believe that ecDNA of irradiated apoptotic lymphocytes is a significant factor of stress-signalling.

  7. Significance Testing Without Truth

    Science.gov (United States)

    2012-07-27

    ICES REPORT 12-34 August 2012 Significance testing without truth by William Perkins, Mark Tygert, and Rachel Ward The Institute for Computational...testing without truth , ICES REPORT 12-34, The Institute for Computational Engineering and Sciences, The University of Texas at Austin, August 2012...2. REPORT TYPE 3. DATES COVERED 00-00-2012 to 00-00-2012 4. TITLE AND SUBTITLE Significance testing without truth 5a. CONTRACT NUMBER 5b

  8. 断层超声显像诊断左肾静脉压迫综合征的意义%The significance of the related factors in diagnosis of left renal vein entrapment syndrome by tomographic ultrasound imaging

    Institute of Scientific and Technical Information of China (English)

    黄枢; 闫建平; 项金凤

    2012-01-01

    Objective To explore the significance of related factors in diagnosis of left renal vein entrapment syndrome by tomographic ultrasound imaging. Methods We randomly collected 70 patients with left renal vein entrapment syndrome and use ultrasound technology to image them,compute the corresponding ratio of LRV diameters of the distended to the narrowed portions and the aortomesenteric angle (A MA, the angle between the aorta and the superior mesenteric artery) .then make 70 randomly selected normal people as control group. Results The fast display rate was 65% by two dimensional ultrasound, which was 100% by TUI. The correlation coefficient between the AMA and the ratio of the inner diameter for patients was - 0. 277 (P < 0.05 ). The inner diameter and the AMA are significantly related. The bigger the ratio of the inner diameter was, the bigger the AMA was. Conclusion Measuring the inner diameter ratio and the AMA is significant to diagnose the left renal vein entrapment syndrome,which is better than two dimensional ultrasound.%目的 探讨断层超声显像(tomographic ultrasound imaging,TUI)诊断左肾静脉压迫综合征相关指标的意义.方法 对70例左肾静脉压迫综合征患者(实验组)进行断层超声诊断并利用相关软件进行定量分析,得出相应的左肾静脉扩张段内径与狭窄段内径比值及肠系膜上动脉与腹主动脉之间的夹角,与对照组进行比较分析.结果 应用二维超声技术快速显示占65%,应用TUI技术快速显示占100%.实验组肠系膜上动脉与腹主动脉之间的角度为(23.58°±3.29°),与对照组角度比差异有统计学意义(P<0.01);实验组内径比值为(4.02 +0.73),与对照组内径比差异有统计学意义(P<0.01).实验组角度与内径比值相关系数(r)为-0.277(P<0.05),表明实验组角度与内径比之间存在负相关,即肠系膜上动脉与腹主动脉之间的角度越小,左肾静脉内径比值越大.结论 利用TUI测量左肾静脉

  9. Expression and significance of transcription factor Ets-1 in type 2 diabetic nephropathy%转录因子Ets-1在2型糖尿病肾病中的表达及其意义

    Institute of Scientific and Technical Information of China (English)

    沈京群; 刘殿阁; 陈涵枝; 丁弘; 汪湜; 周建东

    2012-01-01

    Objective: Ets-1 proto-oncogene is a member of the transcriptional factor family and was identified by homology to the V-ets oncogene. It was recently demonstrated that Ets-l protein interacts with the promoter region of the genes coding for proteinases, including matrix metalloproteinases ( MMPs ), suggesting that it may play an important role in the regulation of MMPs expression. The role of the Ets-l proto-oncogene in diabetic nephropathy ( DN ), where extracellular matrix ( ECM ) accumulation is observed, remains undefined. To elucidate the role of Ets-l in DN,we studied the expression pattern of Ets-l,Matrix metaloproteinase-2 ( MMP-2 ), Tissue Inhibitors of Metalloproteinase-2 ( TIMP-2 ), α- smooth muscle action ( α- SMA ) and type IV collagen ( Col IV ) in matrix remodeling in Type 2 DN. Methods: 25 cases of renal biopsy sections diagnosed as DN were selected. Type 2 diabetes was defined according to Standards of Medical Care in diabetes. 2007/ADA. Meanwhile, 20 cases of renal biopsy specimens diagnosed as minor glomerular abnormalities ( minimal change disease, MCD ) were simultaneously studied as controls. Pathological classifications were made according to the WHO criteria of 1982 for renal pathology or the modified WHO criteria of 1995. Histopathological findings were identified under light microscopy. Using immunohistochemistry and double immunostaining, we investigated the expression of Ets- 1 , MMP-2, TIMP-2, α- SMA, vimentin and collagen type Ⅳ( Col Ⅳ )in type 2 DN. The experimental data was statistically analyzed and statistical significance was achieved at corrected P < 0. 05. Results: 25 DN cases ( male 15, female 10 ) between the ages of 35 and 67 years were observed. 20 MCD patients ( male 12, female 8 ), aged 18-46 years, were noted. By immunnohistochemistry, the expression of Ets-1 in glomeruli and tubulointerstitium was significantly increased in the kidneys obtained from DN group compared with MCD groups ( P < 0. 05 ). Compared to MCD

  10. A Significant Play

    Institute of Scientific and Technical Information of China (English)

    梁海光; 陈明

    2002-01-01

    Yesterday evening, I went to see a play. It was really significant. It was about Zheng Xiaoyue, a very clever and diligent middle school student. Unfortunately, her mother died when she and her brother were very young. Her father was out of work and,

  11. Show Horse Welfare: Horse Show Competitors' Understanding, Awareness, and Perceptions of Equine Welfare.

    Science.gov (United States)

    Voigt, Melissa A; Hiney, Kristina; Richardson, Jennifer C; Waite, Karen; Borron, Abigail; Brady, Colleen M

    2016-01-01

    The purpose of this study was to gain a better understanding of stock-type horse show competitors' understanding of welfare and level of concern for stock-type show horses' welfare. Data were collected through an online questionnaire that included questions relating to (a) interest and general understanding of horse welfare, (b) welfare concerns of the horse show industry and specifically the stock-type horse show industry, (c) decision-making influences, and (d) level of empathic characteristics. The majority of respondents indicated they agree or strongly agree that physical metrics should be a factor when assessing horse welfare, while fewer agreed that behavioral and mental metrics should be a factor. Respondent empathy levels were moderate to high and were positively correlated with the belief that mental and behavioral metrics should be a factor in assessing horse welfare. Respondents indicated the inhumane practices that most often occur at stock-type shows include excessive jerking on reins, excessive spurring, and induced excessive unnatural movement. Additionally, respondents indicated association rules, hired trainers, and hired riding instructors are the most influential regarding the decisions they make related to their horses' care and treatment.

  12. 转录因子Snail在非小细胞肺癌中的表达及意义%Expression and significance of transcription factor Snail in the non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    赵晓华; 张明月; 崔红学; 都彩菊; 刘宝堂

    2011-01-01

    Objective To detect the expression of transcription factor Snail mRNA and protein, and investigate the role of Snail in the development of non-small cell lung cancer (NSCLC). Methods The expression of Snail protein and mRNA in NSCLC and normal lung tissue was detected by immunohistochem-istry and RT-PCR. The relationship of Snail and clinicothological parameters was analyzed. Results The positive rate of Snail mRNA were significantly higher in cancer tissues (82. 7%) than that in normal lung tissues (23. 1%, P 0. 05). Conclusion The carcinogenesis, invasion and metastasis of NSCLC may be correlated to overex-pression of Snail protein and mRNA.%目的 检测转录因子Snail mRNA及蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,探讨Snail在NSCLC发生发展中的作用.方法 采用免疫组织化学、RT-PCR的方法检测NSCLC、癌旁相对正常肺组织(>5cm)中转录因子Snail蛋白及mRNA的表达,并分析其与临床病理参数之间的关系.结果 NSCLC组织中Snail mRNA的阳性表达率(82.7%)高于癌旁相对正常肺组织(23.1%,P<0.05);NSCLC组织中Snail mRNA的阳性表达量(1.368±0.614)高于癌旁相对正常肺组织(0.579±0.217,P<0.05).NSCLC组织中Snail 蛋白的阳性表达率(80.8%)高于癌旁相对正常肺组织(23.1%,P<0.05),且癌组织中Snail蛋白阳性表达率与TNM分期、淋巴结转移有关(P<0.05),而与组织分型、分化程度、吸烟史无关(P>0.05).结论 NSCLC的癌变、侵袭、转移可能与Snail蛋白、mRNA的高表达有关.

  13. Anti-CCP和RF联合检测在诊断RA中的意义%Significance of detecting anti-cyclic citrullinated peptide and rheumatoid factor in the diagnosis of rheumatoid arthritis

    Institute of Scientific and Technical Information of China (English)

    郭玉娟

    2012-01-01

    目的 分析比较抗环瓜氨酸肽抗体(Anti-CCP)和类风湿因子(RF)在类风湿关节炎(RA)中的灵敏度和特异性,探讨将两种指标联合检测在RA诊断中的价值.方法 利用酶联免疫吸附试验和乳胶增强免疫透射比浊法分别测定76例RA 患者,56例非RA 患者血清中的Anti-CCP和RF.结果 Anti-CCP和RF在RA组的阳性率明显高于非RA组.特异度和灵敏度RF分别为76.8%、78.9%,Anti-CCP分别为96.4%、60.5%,将两者联合检测则分别为86.6%、73.1%.结论 Anti-CCP在诊断RA中具有较高的特异性和灵敏度,可作为诊断RA的新的血清学指标.将Anti-CCP和RF联合检测有助于RA的临床诊断.%Objective To analyse the specificily and sensilivily of anli-cyclic cilrullinaled peplide (Anli-CCP) and rheumatoid factor (RF) in the diagnosis of rheumatoid arlhrilis (RA) to invesligale the value when the Iwo anlibodies were used togelher. Methods ELISA and Lalex enhanced immune lurbidimelric were used to deled serum levels of Anli-CCP and RF in 76 palienls wilh RA and 56 non- RA palienls. Results The positive rale of Anli-CCP and RF of palienls wilh RA was significantly higher lhan lhal of non-RA palienls. The diagnostic specificily of RF for RA was 76. 8% and the sensilivily was 78. 9% , whereas the diagnostic specificily of Anti-CCP for RA was 96. 4% and the sensitivity was 60. 5%. The diagnostic specificily of the Anli-CCP and RF was 86. 6% and the sensitivity was 73. 1%. Conclusion The Anti-CCP might have high specificity and sensitivity, which might be used as the new serum sign for the diagnosis of RA. Combined wilh the detection of Anti-CCP and RF , the sensitivity will be higher.

  14. Are studies reporting significant results more likely to be published?

    Science.gov (United States)

    Koletsi, Despina; Karagianni, Anthi; Pandis, Nikolaos; Makou, Margarita; Polychronopoulou, Argy; Eliades, Theodore

    2009-11-01

    Our objective was to assess the hypothesis that there are variations of the proportion of articles reporting a significant effect, with a higher percentage of those articles published in journals with impact factors. The contents of 5 orthodontic journals (American Journal of Orthodontics and Dentofacial Orthopedics, Angle Orthodontist, European Journal of Orthodontics, Journal of Orthodontics, and Orthodontics and Craniofacial Research), published between 2004 and 2008, were hand-searched. Articles with statistical analysis of data were included in the study and classified into 4 categories: behavior and psychology, biomaterials and biomechanics, diagnostic procedures and treatment, and craniofacial growth, morphology, and genetics. In total, 2622 articles were examined, with 1785 included in the analysis. Univariate and multivariate logistic regression analyses were applied with statistical significance as the dependent variable, and whether the journal had an impact factor, the subject, and the year were the independent predictors. A higher percentage of articles showed significant results relative to those without significant associations (on average, 88% vs 12%) for those journals. Overall, these journals published significantly more studies with significant results, ranging from 75% to 90% (P = 0.02). Multivariate modeling showed that journals with impact factors had a 100% increased probability of publishing a statistically significant result compared with journals with no impact factor (odds ratio [OR], 1.99; 95% CI, 1.19-3.31). Compared with articles on biomaterials and biomechanics, all other subject categories showed lower probabilities of significant results. Nonsignificant findings in behavior and psychology and diagnosis and treatment were 1.8 (OR, 1.75; 95% CI, 1.51-2.67) and 3.5 (OR, 3.50; 95% CI, 2.27-5.37) times more likely to be published, respectively. Journals seem to prefer reporting significant results; this might be because of authors

  15. Significance analysis of prognostic signatures.

    Directory of Open Access Journals (Sweden)

    Andrew H Beck

    Full Text Available A major goal in translational cancer research is to identify biological signatures driving cancer progression and metastasis. A common technique applied in genomics research is to cluster patients using gene expression data from a candidate prognostic gene set, and if the resulting clusters show statistically significant outcome stratification, to associate the gene set with prognosis, suggesting its biological and clinical importance. Recent work has questioned the validity of this approach by showing in several breast cancer data sets that "random" gene sets tend to cluster patients into prognostically variable subgroups. This work suggests that new rigorous statistical methods are needed to identify biologically informative prognostic gene sets. To address this problem, we developed Significance Analysis of Prognostic Signatures (SAPS which integrates standard prognostic tests with a new prognostic significance test based on stratifying patients into prognostic subtypes with random gene sets. SAPS ensures that a significant gene set is not only able to stratify patients into prognostically variable groups, but is also enriched for genes showing strong univariate associations with patient prognosis, and performs significantly better than random gene sets. We use SAPS to perform a large meta-analysis (the largest completed to date of prognostic pathways in breast and ovarian cancer and their molecular subtypes. Our analyses show that only a small subset of the gene sets found statistically significant using standard measures achieve significance by SAPS. We identify new prognostic signatures in breast and ovarian cancer and their corresponding molecular subtypes, and we show that prognostic signatures in ER negative breast cancer are more similar to prognostic signatures in ovarian cancer than to prognostic signatures in ER positive breast cancer. SAPS is a powerful new method for deriving robust prognostic biological signatures from clinically

  16. TrkB和BDNF在胃癌组织中的表达及意义%Expression and significance of tyrosine kinase receptor B and brain-derived neurotrophic factor in gastric carcinoma

    Institute of Scientific and Technical Information of China (English)

    刘天卿; 任海军; 温爽; 张众

    2008-01-01

    目的 观察酪氨酸激酶受体B(TrkB)及其配体脑源性神经营养因子(BDNF)在胃癌组织、癌旁组织和正常胃黏膜组织中的表达情况,探讨TrkB和BDNF与胃癌临床病理参数的关系.方法 采用免疫组织化学sP法检测64例原发性胃癌组织、癌旁组织,34例有淋巴结转移患者中对应的阳性淋巴结和20例正常胃黏膜组织中TrkB和BDNF的表达,分析其与临床病理特征的关系.结果 胃癌组织中TrkB和BDNF阳性表达率分别为60.9%、59.4%,而癌旁组织和正常胃黏膜组织中无一例表达.TrkB和BDNF表达与患者性别、年龄、肿瘤分化程度等无关,而与浸润深度、淋巴结转移和TNM分期有关.浸润至胃壁全层、有淋巴结转移和TNM分期Ⅲ~Ⅳ期的TrkB和BDNF阳性表达率明显高于未浸润至胃壁全层、无淋巴结转移和TNM分期Ⅰ~Ⅱ期(P0.05).结论 TrkB和BDNF表达与胃癌发生、发展密切相关,二者的表达增强提示肿瘤可能已发生局部侵袭和远处转移.%Objective To investigate the expressions of tyrosine kinase receptor (Trk)B and brain-derived neurotrophic factor (BDNF) protein in human gastric careinoma and compare them with those in epithelial cells of normal mucous, in order to evaluate their clinicopathological significance. Method The expressions of TrkB and BDNF protein in tumor tissues, matched with para-tumor mueosal tissues from 64 cases with gastric carcinoma and normal mucous of 20 cases were observed immunohistochemically and related to some of the clinicopathological parameters. Results The positive rates of TrkB and BDNF protein in tumor tissues were 60.9% and 59.4% respectively, but there was negative in matched para-tumor mueosal tissues and normal mucosal tissues. TrkB and BDNF protein expressions were related to invasive depth,lymph node metastasis and TNM stage of cancer, but not to sex, age and degrees of cancerous differentiation.The positive rates of TrkB and BDNF protein in cases with

  17. Significant Tsunami Events

    Science.gov (United States)

    Dunbar, P. K.; Furtney, M.; McLean, S. J.; Sweeney, A. D.

    2014-12-01

    Tsunamis have inflicted death and destruction on the coastlines of the world throughout history. The occurrence of tsunamis and the resulting effects have been collected and studied as far back as the second millennium B.C. The knowledge gained from cataloging and examining these events has led to significant changes in our understanding of tsunamis, tsunami sources, and methods to mitigate the effects of tsunamis. The most significant, not surprisingly, are often the most devastating, such as the 2011 Tohoku, Japan earthquake and tsunami. The goal of this poster is to give a brief overview of the occurrence of tsunamis and then focus specifically on several significant tsunamis. There are various criteria to determine the most significant tsunamis: the number of deaths, amount of damage, maximum runup height, had a major impact on tsunami science or policy, etc. As a result, descriptions will include some of the most costly (2011 Tohoku, Japan), the most deadly (2004 Sumatra, 1883 Krakatau), and the highest runup ever observed (1958 Lituya Bay, Alaska). The discovery of the Cascadia subduction zone as the source of the 1700 Japanese "Orphan" tsunami and a future tsunami threat to the U.S. northwest coast, contributed to the decision to form the U.S. National Tsunami Hazard Mitigation Program. The great Lisbon earthquake of 1755 marked the beginning of the modern era of seismology. Knowledge gained from the 1964 Alaska earthquake and tsunami helped confirm the theory of plate tectonics. The 1946 Alaska, 1952 Kuril Islands, 1960 Chile, 1964 Alaska, and the 2004 Banda Aceh, tsunamis all resulted in warning centers or systems being established.The data descriptions on this poster were extracted from NOAA's National Geophysical Data Center (NGDC) global historical tsunami database. Additional information about these tsunamis, as well as water level data can be found by accessing the NGDC website www.ngdc.noaa.gov/hazard/

  18. “以小说见才学者”辨正及其小说史叙述意义--兼及“才学小说”的概念使用%Reviewing Lu Xun’s Concept of“Showing Caixue Through Fiction Writing”and Its Significance for Constructing Chinese Fiction History:With a View on How to Apply the Concept of Caixue Novels

    Institute of Scientific and Technical Information of China (English)

    温庆新

    2014-01-01

    In his Zhonguo xiaoshuo shilue中国小说史略,Lu Xun鲁迅 devotes a special chapter to these four novels:Yesou puyan野叟曝言,Jinghua yuan镜花缘,Yanshan waishi燕山外史,and Yinshi鑘史,and identifies them as novels“showing caixue才学[academic and artistic achievements] through fiction writing”. Although this identification is not based on generic and stylistic criteria and those novels are still classified into the types of Shenmo神魔novels and Shiqing世情 novels respec-tively,what Lu Xun really wants to do here is to highlight the commonality in the creative motivation shared by their authors who manage to get their own aspirations and feelings expressed between the lines in their novels;and is to explore the significance of these novels for constructing a history of Chi-nese fiction. This is the direct result from his construction of the law governing the evolution of Chinese fiction:“novels,which were started as a by-product of literary creation,become one of the profession-al literary pursuits.”There is,however,some tension between the rationale behind and the exemplifi-cation for Lu Xun’s identification of novels“showing caixue through fiction writing”,which is inevita-bly skewed towards focusing on the novelists’ writing intentions. Researchers misunderstood Lu Xun’s concept of“showing caixue through fiction writing”merely as“caixue novels”.%《中国小说史略》专篇讨论《野叟曝言》、《镜花缘》、《燕山外史》及《鑘史》四部小说并名之“以小说见才学者”时,并非基于类型归纳或文体判断而言。这些小说依旧被归入“神魔小说”或才子佳人型的“世情小说”中。鲁迅此举实为强调此类小说写作者“自寓”、“寄慨”的创作倾向,突出“以小说见才学者”创作现象于小说史的叙述意义,是其建构“从无意为小说向有意为小说演进”的小说史演进规律之目的使然。这些意图及

  19. A Significant Step Forward

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Australia officially ratified the Kyoto Protocol on December 3,the first act of its new government under Prime Minister Kevin Rudd.Rudd signed the instrument of ratification the very day he was sworn in by Australia’s Governor General Michael Jeffery. This is a significant step in Australia’s efforts to fight climate change domestically and with the international community,Rudd said in a statement.The Australian Government will do everything in its power to help Australia meet its Kyoto obligations,he added.

  20. On significance of VLBI/Gaia offsets

    CERN Document Server

    Petrov, L

    2016-01-01

    We have cross matched the Gaia Data Release 1 secondary dataset that contains positions of 1.14 billion objects against the most complete to date catalogue of VLBI positions of 11.4 thousand sources, almost exclusively active galactic nuclei. We found 6,064 matches, i.e. 53% radio objects. The median uncertainty of VLBI positions is a factor of 4 smaller than the median uncertainties of their optical counterparts. Our analysis shows that the distribution of normalized arc lengths significantly deviates from Rayleighian shape with an excess of objects with small normalized arc lengths and with a number of outliers. We found that 8% matches have radio optical offsets significant at 99% confidence level. Therefore, we conclude there exists a population of objects with genuine offsets between centroids of radio and optical emission.

  1. 甲状腺转录因子-1在肺癌组织中的表达及临床意义%Expression and Clinical Significance of Thyroid Transcription Factor 1 in Lung Cancer Tissues

    Institute of Scientific and Technical Information of China (English)

    黄克锋; 冼磊

    2014-01-01

    Objective To investigate the expression and diagnostic significance of thyroid transcription factor 1( TTF-1) in lung cancer tissues .Methods The expression of TTF-1 was detected by immunohistochemical staining in 50 cases of primary lung cancer ( including 23 cases of adenocarcinoma ,14 cases of squamous carcinoma ,9 cases of small-cell lung cancer ,and 4 cases of large-cell lung cancer ) and para-carcinoma tissues as well as 21 cases of metastatic lung adenocarcinomas from digestive tract and ovaries .The diagnostic and differential diagnostic values of TTF-1 in lung cancer were assessed .Results The positive expression rates of TTF-1 were 73 .9%( 17/23 ) in adenocarcinoma ,0 in squamouse cell carcinoma,77.8%(7/9)in small-cell lung cancer tissues and 50.0%(2/4)in large-cell lung cancer tissues,0 in metastatic lung adenocarcinomas ,respectively.The positive expression rates of TTF-1 in adenocarcinoma and small-cell lung cancer tissues were higher than that in squamouse cell carcinoma tissues (P0.05).Conclusion TTF-1 can be used as a specific indicator for the differential diagnosis of primary or secondary lung adenocarcinomas ,and it provides some references for the judgement of histological type of primary lung cancer .%目的:探讨甲状腺转录因子-1(TTF-1)在肺癌组织中表达情况其临床诊断意义。方法采用免疫组织化学法分别检测50例原发性肺癌(腺癌23例、鳞癌14例、肺小细胞癌9例、肺大细胞癌4例)组织、癌旁组织及21例来源于胃肠道或卵巢转移性肺腺癌组织标本中TTF-1的表达情况,并分析TTF-1在肺癌诊断与鉴别诊断中的意义。结果腺癌、鳞癌、肺小细胞癌、肺大细胞癌、转移性肺腺癌组织TTF-1表达阳性率分别为73.9%(17/23)、0、77.8%(7/9)、50.0%(2/4)、0,腺癌、肺小细胞癌组织 TTF-1表达阳性率较鳞癌高( P<0.05)。原发肺癌的癌旁组织中,正常或增生肺泡上皮大部

  2. 成纤维细胞生长因子2、成纤维细胞生长因子受体4蛋白在人甲状腺乳头状癌中的表达及意义%Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-4 in human papillary thyroid carcinoma and their significances

    Institute of Scientific and Technical Information of China (English)

    李倩; 李莉; 郭淑芹; 张云良; 孔凡强; 李菲; 康军朋; 吴靖芳; 高福禄

    2014-01-01

    Objective To investigate the expression of fibroblast growth factor-2 (FGF-2) and fibroblast growth factor receptor-4 ( FGFR-4 ) in the papillary thyroid carcinomas ( PTC ) and clinical significance . Methods Immunohistochemistry and Western blotting for the expression of FGF-2 and FGFR-4 were performed in 89 cases of PTC and 30 cases of normal thyroid tissues ( NTT) adjacent to the tumors .Results Immunohistochemistry results showed that , FGF-2 and FGFR-4 expressions were high in thyroid carcinoma (P0.05).Analyzed by Western blotting technique ,FGF-2 and FGFR-4 expressions in thyroid carcinoma were significantly higher than that in normal tissue ,with decrease of cancer degree of tissue differentiation and significantly up regulated expression (P<0.05).Expressions of FGF-2 and FGFR-4 were in a positive linear correlation in the disease (rs=0.434,P<0.01).Conclusion The expressions of FGF-2 and FGFR-4 are correlated with papillary thyroid cancer and they participated in the process of invasion and metastasis , both of which have a positive synergistic effect .The degree of malignancy and biological behavior are meaningful and comprehensive indicators ,which provide a theoretical basis for the subsequent experimental studies of cellular and molecular biology .%目的:探讨甲状腺乳头状癌( PTC)组织中成纤维细胞生长因子2( FGF-2)和成纤维细胞生长因子受体4(FGFR-4)的表达及其是否存在相关性。方法收集89例甲状腺乳头状癌及30例癌旁正常甲状腺组织标本,采用免疫组织化学和免疫印迹法( Western blotting )检测FGF-2和FGFR-4蛋白在标本中的表达,并进行统计学分析。结果免疫组织化学方法结果显示,与癌旁正常组织相比,FGF-2及FGFR-4在人类甲状腺乳头状癌组织中均高表达(P<0.01, P<0.01),两者差异有统计学意义;FGF-2和FGFR-4在甲状腺乳头状癌中的表达与淋巴结转移(χ2=14.798,P<0.01;χ2

  3. [Hypertriglyceridemia: concept and clinical significance].

    Science.gov (United States)

    Hirano, Tsutomu

    2013-09-01

    Hypertriglyceridemia is a common lipid disorder as well as hypercholesterolemia. However clinical significance of hypertriglyceridemia is not fully understood because of its heterogeneous lipoprotein phenotypes and complex etiology. Severe hypertriglyceridemia increases the risk for pancreatitis, whereas mild or moderate hypertriglyceridemia may be a risk factor for cardiovascular disease. Patients with hypertriglyceridemia are usually accompanied by other cardiovascular related disorders, such as central obesity, type 2 diabetes, and liver steatosis. Ectopic fat accumulation is often seen in hypertriglyceridemic subjects and various organ injuries are developed by the lipotoxicity. Hypertriglyceridemia is strongly associated with remnant lipoprotein accumulation, increased small dense LDL, and low HDL-cholesterol. All these lipid abnormalities are recognized as cardiovascular risk factors. The pathophysiology of lipoprotein metabolism related to the hypertriglyceridemia is summarized in this brief review.

  4. Predicting significant torso trauma.

    Science.gov (United States)

    Nirula, Ram; Talmor, Daniel; Brasel, Karen

    2005-07-01

    Identification of motor vehicle crash (MVC) characteristics associated with thoracoabdominal injury would advance the development of automatic crash notification systems (ACNS) by improving triage and response times. Our objective was to determine the relationships between MVC characteristics and thoracoabdominal trauma to develop a torso injury probability model. Drivers involved in crashes from 1993 to 2001 within the National Automotive Sampling System were reviewed. Relationships between torso injury and MVC characteristics were assessed using multivariate logistic regression. Receiver operating characteristic curves were used to compare the model to current ACNS models. There were a total of 56,466 drivers. Age, ejection, braking, avoidance, velocity, restraints, passenger-side impact, rollover, and vehicle weight and type were associated with injury (p < 0.05). The area under the receiver operating characteristic curve (83.9) was significantly greater than current ACNS models. We have developed a thoracoabdominal injury probability model that may improve patient triage when used with ACNS.

  5. 类风湿性关节炎中肿瘤坏死因子α的表达及意义%The Expression and Significance of Tumor Necrosis Factor-α in Rheumatoid Arthritis

    Institute of Scientific and Technical Information of China (English)

    陈如平

    2011-01-01

    类风湿性关节炎(RA)是一种慢性炎症性疾病,它的发病与多种细胞因子有关,其中肿瘤坏死因子(TNF)是引起该病的最主要的因子之一.研究表明,TNF-α在RA的发病机制中起着重要作用,是导致炎性反应的持续发生和软骨与骨的破坏的重要促炎性细胞因子.抗TNF-α药物在临床上治疗RA有良好的效果,对控制病情和改善预后非常重要.%Rheumatoid arthritis( RA )is a chronic inflammation disease, its morbidity related with many kinds of cell factors,the tumor necrosis factor( TNF )is one of the most main factors which causes this sickness. Many researches indicated that TNF-α is playing the influential role in rheumatic arthritis' pathogenesis,it is an important inflammatotry cell factor which causes the inflammation occurs continually and presses the cartilage and bone's destruction. The anti-TNF-α medicine has a good effect in treating rheumatic arthritis on clinical,it is important to control the condition and the improvement prognosis of RA.

  6. 核因子κB与支气管哮喘发病关系及其临床意义%Relationship between nuclear factor-κB and pathogenesy of bronchial asthma and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    顾丹; 白春学

    2008-01-01

    核因子κB(nuclear factor-κB,NF-κB)是一种调控基因表达的蛋白质核转录因子.它可以调控与炎症免疫反应有关的炎症因子、细胞因子、氧化应激相关酶等的基因表达,从而参与肺部的炎症及免疫反应,在支气管哮喘(简称哮喘)的发病机制中发挥着重要作用.NF-κB抑制剂通过阻断NF-κB激活通路上的多个环节,以达到治疗哮喘的目的,将会成为哮喘治疗研究的热点.%Nuclear factor-κB(NF-κB)is a nuclear transcription factor which regulates gene expression.It can regulate gene expression of inflammatory and immune response,such as inflammatory factor,cytokine,enzymes related to oxidative stress,et al.Thus it joins in the imflammatory and immune response of lung and takes an active part in the pathogenesy of bronchial asthma(asthma).NF-κB inhibitor treats asthma by blocking the multiple links of NF-κB activation.It will soon become the hot spot of research on asthma treatment.

  7. MMP-9和 TGFβ1在宫颈鳞癌中的表达和意义%The expression and significance of matrixmetalloproteinase-9 (MMP-9) andtransforming growth factorβ1 (TGF-βl) incervical cancer Abstract

    Institute of Scientific and Technical Information of China (English)

    沈浩明; 杨友义; 吴白平; 向延娥

    2015-01-01

    目的:分别检测宫颈分泌物、血液和宫颈病变组织中的基质金属蛋白酶-9(MMP-9)和转化生长因子β1(TGF-βl)的表达情况,探讨其在宫颈病变形成及演进过程中的意义。方法:应用 ELISA 法检测66例病人(其中慢性宫颈炎组织10例、CINcervicalintraepithelialneoplasia 宫颈上皮内瘤变24例,宫颈鳞癌32例)的宫颈分泌物和血清中的 TGF-βl 和 MMP-9的表达水平。应用免疫组织化学 SP 法检测相对应病人石蜡包埋的不同病变宫颈组织中TGF-βl 和 MMP-9的表达。结果:1.在血清和宫颈分泌物中 TGF-βl 和 MMP-9的浓度与宫颈癌密切相关:慢性宫颈炎组、宫颈上皮内瘤变(CIN)组和宫颈鳞癌(CxCa)组的样本中 TGF-βl 和 MMP-9的浓度逐级升高,提示随着宫颈癌恶性程度的增加,TGF-βl 和 MMP-9的表达明显增强。血清和宫颈分泌物中 MMP-9和 TGF-β1与其切片免疫组化结果相吻合。随着 MMP-9在宫颈分泌物和血清中表达的增强,TGF-β1的表达也明显增高,两者呈正相关。2.TGF-βl 和 MMP-9表达与宫颈癌的病理和临床分类的关系:在宫颈癌组织中有淋巴结转移的 MMP-9和 TGFβ1的表达阳性率显著高于无淋巴结转移的。在宫颈癌≥Ⅱ b 期的 MMP-9和 TGF-βl 的表达阳性率显著高于≤Ⅱ a 期的。MMP-9和 TGF-βl 与宫颈癌肿瘤的大小和组织病理学分级无显著性差异。结论:TGF-βl 和 MMP-9在宫颈病变、宫颈分泌物和血清的中表达与病变良恶性有关,TGF-βl 和 MMP-9高表达是宫颈恶性肿瘤的表现之一,并且 TGF-βl和 MMP-9在宫颈分泌物或血清中的表达具有显著相关性。%Objesctive The expression of matrix metalloproteinase-9 (MMP-9) and transforming growth factorβ1 (TGF-βl) were detectedin cervical secretions, blood andcervical lesions, in order to investigate the significance in thedevelopment ofcervical lesions. Methods 66 cases (including 10

  8. Meaning and significance of

    Directory of Open Access Journals (Sweden)

    Ph D Student Roman Mihaela

    2011-05-01

    Full Text Available The concept of "public accountability" is a challenge for political science as a new concept in this area in full debate and developement ,both in theory and practice. This paper is a theoretical approach of displaying some definitions, relevant meanings and significance odf the concept in political science. The importance of this concept is that although originally it was used as a tool to improve effectiveness and eficiency of public governance, it has gradually become a purpose it itself. "Accountability" has become an image of good governance first in the United States of America then in the European Union.Nevertheless,the concept is vaguely defined and provides ambiguous images of good governance.This paper begins with the presentation of some general meanings of the concept as they emerge from specialized dictionaries and ancyclopaedies and continues with the meanings developed in political science. The concept of "public accontability" is rooted in economics and management literature,becoming increasingly relevant in today's political science both in theory and discourse as well as in practice in formulating and evaluating public policies. A first conclusin that emerges from, the analysis of the evolution of this term is that it requires a conceptual clarification in political science. A clear definition will then enable an appropriate model of proving the system of public accountability in formulating and assessing public policies, in order to implement a system of assessment and monitoring thereof.

  9. Baryon form factors

    CERN Document Server

    Kubis, B; Meißner, Ulf G; Mei{\\ss}ner, Ulf-G.

    1999-01-01

    We calculate the form factors of the baryon octet in the framework of heavy baryon chiral perturbation theory. The calculated charge radius of the show that kaon loop effects can play a significant role in the neutron electric form factor. Furthermore. we derive generalized Caldi-Pagels relations between various charge radii which are free of chiral loop effects.

  10. Risk factors and clinical significance of severe mycoplasma pneumoniae pneumonia%重症肺炎支原体肺炎危险因素分析及临床意义

    Institute of Scientific and Technical Information of China (English)

    荆小袁

    2016-01-01

    Mycoplasma pneumoniae pneumonias are common in children.In recent years,the incidence of refractory mycoplasma pneumonia has been increasing,and some of the severe children have a poor response to macrolide antibiotics.However,the effect of glucoconicoid therapy may have unexpected results.The recognition of severe mycoplasma pneumonia risk factors may contribute to intervent with glucocorticoid early,reduce the occurrence of severe mycoplasma pneumonia,and improve the prognosis.Clinical studies have found that clinical manifestations,imaging findings,laboratory tests and other factors are related to the occurrence of severe mycoplasma pneumonia.In this paper,the risk factors that may be associated with accurrence of severe mycoplasma pneumonias are reviewed.%肺炎支原体肺炎在儿童中较为常见.近年来难治性肺炎支原体肺炎日趋增多,其中部分重症患几虽经大环内酯类抗生素治疗仍难见效.然而糖皮质激素治疗有意想不到的效果.对重症肺炎支原体肺炎危险因素的识别,可能有助于早期予以激素干预,改善预后.临床研究发现,临床表现、影像学表现及实验室检查等多种指标与重症肺炎支原体肺炎的发生相关.该文对目前国内外研究所涉及的、可能与重症肺炎支原体肺炎发生相关的因素进行综述.

  11. Social significance of community structure: statistical view.

    Science.gov (United States)

    Li, Hui-Jia; Daniels, Jasmine J

    2015-01-01

    Community structure analysis is a powerful tool for social networks that can simplify their topological and functional analysis considerably. However, since community detection methods have random factors and real social networks obtained from complex systems always contain error edges, evaluating the significance of a partitioned community structure is an urgent and important question. In this paper, integrating the specific characteristics of real society, we present a framework to analyze the significance of a social community. The dynamics of social interactions are modeled by identifying social leaders and corresponding hierarchical structures. Instead of a direct comparison with the average outcome of a random model, we compute the similarity of a given node with the leader by the number of common neighbors. To determine the membership vector, an efficient community detection algorithm is proposed based on the position of the nodes and their corresponding leaders. Then, using a log-likelihood score, the tightness of the community can be derived. Based on the distribution of community tightness, we establish a connection between p-value theory and network analysis, and then we obtain a significance measure of statistical form . Finally, the framework is applied to both benchmark networks and real social networks. Experimental results show that our work can be used in many fields, such as determining the optimal number of communities, analyzing the social significance of a given community, comparing the performance among various algorithms, etc.

  12. Social significance of community structure: Statistical view

    Science.gov (United States)

    Li, Hui-Jia; Daniels, Jasmine J.

    2015-01-01

    Community structure analysis is a powerful tool for social networks that can simplify their topological and functional analysis considerably. However, since community detection methods have random factors and real social networks obtained from complex systems always contain error edges, evaluating the significance of a partitioned community structure is an urgent and important question. In this paper, integrating the specific characteristics of real society, we present a framework to analyze the significance of a social community. The dynamics of social interactions are modeled by identifying social leaders and corresponding hierarchical structures. Instead of a direct comparison with the average outcome of a random model, we compute the similarity of a given node with the leader by the number of common neighbors. To determine the membership vector, an efficient community detection algorithm is proposed based on the position of the nodes and their corresponding leaders. Then, using a log-likelihood score, the tightness of the community can be derived. Based on the distribution of community tightness, we establish a connection between p -value theory and network analysis, and then we obtain a significance measure of statistical form . Finally, the framework is applied to both benchmark networks and real social networks. Experimental results show that our work can be used in many fields, such as determining the optimal number of communities, analyzing the social significance of a given community, comparing the performance among various algorithms, etc.

  13. Clinical Significance of Determination of Serum Cystatin C (Cys C),Transforming Growth Factor (TGF-β) and Hepatocyte Growth Factor (HGF) Levels in Patients with DM2 Complicated with Nephropathy%DN患者血清Cys C、TGF-β1和HGF检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    武幸福; 陈立侠

    2011-01-01

    目的:探讨了2型糖尿病并发肾病(DN)患者血清胱抑素C(Cys C)、转化生长因子(TGF-β1)和肝细胞因子(HGF)水平的变化及临床意义.方法:对102例2型糖尿病(DM2)患者(其中并发DN 33例,未并发DN 69例)应用酶联法和免疫比浊法进行了Cys C、TGF-β1和HGF水平测定,并与35名正常健康人作比较.结果:DN组血清CysC、TGF-β1水平非常显著地高于正常人组(P<0.01),而HGF水平又非常显著地低于正常人组(P<0.01).血清HGF水平与Cys C、TGF-β1水平呈负相关(r=-0.482、-0.450,P<0.01).结论:检测DM2患者血清Cys C,TGF-β1和HGF水平的变化对早期DN的发生和病情发展程度有重要的临床意义.%0bjective To study the clinical significance of determination of serum Cystatin C, transforming growth factor and hepatocyte growth factor levels in patients with DM2 complicated with nephropathy. Methods Serum TGF-β, HGF (with ELISA), Cys C (with immunoturbidimetry) levels were determined in 102 patients with type2 diabetis (33 with diabetic nephropathy and 69 without nephropathy) and 35controls. Results The serum levels of Cys C ,TGF-β contents in patients with nephropathy were significantly higher than those in the controls (P<0. 01), while the serum HGF levels were significantly lower than those in controls (P <0.01). Serum HGF levels were negativiely correlated with serum Cys C and TGF-β1 levels (r= -0.482, -0.450, P<0. 01). Conclusion Serum Cys C, TGF-β1 and HGF could be used as sensitive markers for early diagnosis of development of diabetic nephropathy.

  14. Human error: A significant information security issue

    Energy Technology Data Exchange (ETDEWEB)

    Banks, W.W.

    1994-12-31

    One of the major threats to information security human error is often ignored or dismissed with statements such as {open_quotes}There is not much we can do about it.{close_quotes} This type of thinking runs counter to reality because studies have shown that, of all systems threats, human error has the highest probability of occurring and that, with professional assistance, human errors can be prevented or significantly reduced Security analysts often overlook human error as a major threat; however, other professionals such as human factors engineers are trained to deal with these probabilistic occurrences and mitigate them. In a recent study 55% of the respondents surveyed considered human error as the most important security threat. Documentation exists to show that human error was a major cause of the consequences suffered at Three Mile Island, Chernobyl, Bhopal, and the Exxon tanker, Valdez. Ironically, causes of human error can usually be quickly and easily eliminated.

  15. [The prognostic significance of brain-derived neurotrophic factor (BDNF) for phobic anxiety disorders, vegetative and cognitive impairments during conservative treatment including adaptol of some functional and organic diseases of nervous system].

    Science.gov (United States)

    Zhivolupov, S A; Samartsev, I N; Marchenko, A A; Puliatkina, O V

    2012-01-01

    We have studied the efficacy of adaptol in the treatment of 45 patients with somatoform dysfunction of the autonomic nervous system and 30 patients with closed head injury. The condition of patients during the treatment was evaluated with clinical and neuropsychological scales. The serum level of BDNF before and after the treatment has been studied as well. Adaptol has been shown to enhance the production of BDNF, reduce significantly the intensity of anxiety, autonomic disorders and improve intellectual processes. The dose-dependent effect of the drug has been demonstrated. In conclusion, adaptol can be recommended for treatment of diseases that demand stimulation of neuroplasticity in the CNS.

  16. Reticulocyte production index as a predictor of clinically significant anemia in chronic hepatitis C patients receiving pegylated interferon combination therapy

    Directory of Open Access Journals (Sweden)

    Sheng-Lei Yan

    2016-03-01

    Conclusion: Besides old age and low pretreatment hemoglobin level, our study showed that a reticulocyte production index < 0.9% at Week 4 was a significant factor associated with clinically significant anemia during pegylated interferon combination treatment.

  17. Expression and significance of heparin binding-epidermal growth factor-like growth factor in paclitaxel-resistant ovarian cancer%肝素结合表皮生长因子在紫杉醇耐药卵巢上皮性癌细胞和组织中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    汤小晗; 卢美松; 李翠萍; 邓锁; 李萌

    2014-01-01

    均有统计学意义(P<0.01)。(2)体内实验结果:接种A2780和A2780/Taxol细胞的裸鼠移植瘤组织中HB-EGF蛋白的表达水平分别为(5.0±2.2)、(10.8±3.3)分,两者比较,差异有统计学意义(P<0.01)。A2780/Taxol+CRM197组肿瘤体积和肿瘤质量分别为(546±85)mm3和(0.56±0.09)g,明显低于A2780/Taxol组的(1840±145)mm3和(1.76±0.23)g,A2780+CRM197组肿瘤体积和肿瘤质量分别为(818±63)mm3和(0.74±0.15)g,明显低于A2780组的(1355±119)mm3和(1.31±0.27)g,分别比较,差异均有统计学意义(P<0.05)。A2780/Taxol+CRM197组的抑瘤率为68%,高于A2780+CRM197组的43%;A2780/Taxol+CRM197组的抑瘤率为68%,高于A2780+CRM197组的43%,分别比较,差异均有统计学意义(P<0.01)。结论紫杉醇耐药的卵巢癌细胞和组织中HB-EGF蛋白均高表达,与卵巢癌对紫杉醇的耐药性相关。抑制HB-EGF蛋白的表达可有效促进紫杉醇耐药卵巢癌细胞凋亡,抑制其生长。%Objective To examine the expression of heparin binding-epidermal growth factor-like growth factor (HB-EGF) in paclitaxel-resistant ovarian cancer and elucidate the relationship between HB-EGF and the resistance of ovarian cancer to paclitaxel. Methods The human ovarian carcinoma cell line A2780 and the paclitaxel-resistant human ovarian carcinoma cell line A2780/Taxol were cultured in vitro. Western blot was used to dectect the expression of HB-EGF protein in A2780 and A2780/Taxol groups. The A2780 cells were treated with cross-reacting material 197 (CRM197 and A2780 + CRM197 group) or dimethyl sulphoxide (DMSO;A2780 group), while the A2780/Taxol cells were treated with CRM197 (A2780/Taxol+CRM197 group) or DMSO (A2780/Taxol group). The effects of CRM197 on growth and proliferation was tested by methyl thiazolyl tetrazolium( MTT) and the results were showed as absorbance (A).The effects of CRM197 on cell cycles was tested by flow cytometry, while the effects of

  18. Expression and Clinical Significance of Transforming Growth Factor β1 and Transforming Growth Factorβ2 in Middle Ear Effusions in Secretory Otitis Media%TGF -β1、TGF -β2在分泌性中耳炎患者中耳积液中的表达和临床意义

    Institute of Scientific and Technical Information of China (English)

    张帆; 袁琨; 肖伯奎; 胡姚; 蔡菁; 张晨星

    2015-01-01

    Objective To investigate the role of transforming growth factor β1( TGF - β1)and transforming growth factor β2(TGF - β2)in the pathogenesis of secretory otitis media(SOM). Methods The expression levels of TGF - β1 and TGF - β2 in middle ear effusions(MEEs)and blood plasma were measured in 56 patients(61 ears)with SOM as well as 32 normal volunteers using enzyme linked immunosorbent assay(ELISA). Results The positive rates of TGF - β1 and TGF - β2 in MEEs and plasma in SOM patients as well as normal plasma were all 100% . The con-centration of TGF - β1 in MEEs in patients with acute SOM was significantly lower than that in patients with chronic SOM(P ﹤ 0. 05). The concentration of TGF - β2 in MEEs in patients with acute SOM was significantly higher than that in patients with chronic SOM(P ﹤ 0. 05). There was no significant difference in the concentration of TGF - β1 in plasma between patients with acute SOM and normal volunteers(P ﹥ 0. 05). There was significant difference in the concentration of TGF - β2 in plasma between patients with acute SOM and normal volunteers(P ﹤ 0. 05). There was significant difference in the concentration of TGF - β1 in plasma between patients with chronic SOM and normal vol-unteers(P ﹤ 0. 05). There was no significant difference in the concentration of TGF - β2 in plasma between patients with acute SOM and normal volunteers(P ﹥ 0. 05). Conclusion TGF - β1 and TGF - β2 both participate in patho-genesis of SOM. TGF - β1 may play a role in the chronic course of SOM and TGF - β2 may be an important role in the early stage of SOM.%目的:探讨转化生长因子-β1(transforming growth factor β1,TGF -β1)和转化生长因子-β2(transforming growth factor β2,TGF -β2)在分泌性中耳炎(secretory otitis media,SOM)发病过程中的作用。方法采用双抗体夹心酶联免疫吸附试验法( enzyme linked immunosorbent assay ELISA)检测56例(61只耳) SOM 患者中

  19. Influence and Significance of Venlafaxine on Basic Fibroblast Growth Factor(bFGF),Vascular Endothelialgrowth Factor (VEGF),Matrix Metalloproteinases-9(MMP-9)Levels in the First-episode Patients with Major Depressive Disorder%文拉法辛对首发抑郁障碍患者血清bFGF、VEGF、MMP-9的影响及意义

    Institute of Scientific and Technical Information of China (English)

    韩毅; 陈涛平; 王丽莉; 左津淮

    2015-01-01

    目的探讨文拉法辛对首发抑郁障碍患者血清碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、血管内皮细胞生长因子(vascular endothelialgrowth factor,VEGF)、基质金属蛋白酶-9(matrix metal oproteinases-9,MMP)水平的影响及意义。方法采用酶联免疫(ELISA)方法检测38例抑郁患者文拉法辛治疗前及治疗4w后和34名正常对照bFGF、VEGF、MMP-9水平;采用24项汉密尔顿抑郁量表(HAMD24)、汉密尔顿焦虑量表(HMMA)评定治疗前及治疗4w后的抑郁、焦虑症状,应用TESS副反应量表记录药物副反应。结果实验组血清bFGF、VEGF、MMP-9水平治疗前及治疗4w后差异无统计学意义(跃0.05)但均高于对照组,差异有统计学意义(0.05). The cor elation coef icients between the serum bFGF,VEGF,MMP-9 in level and total scores of HAMD-24 and HAMA in patients were not significant ( >0.05). Conclusion bFGF, VEGF, MMP-9 may be involved in the pathophysiology of depression.

  20. 呼出气冷凝液中pH测定的影响因素及pH检测的临床意义%Influential factors and clinical significance of pH in exhaled breath condensate

    Institute of Scientific and Technical Information of China (English)

    余苏云; 刘翱

    2009-01-01

    呼出气冷凝液检测技术为一项新型的呼吸道炎症状态检测方法.近年来国外对其研究进展很快,临床应用得到了很大发展,而其中以pH值检测技术应用最为广泛,目前广泛应用于哮喘、慢性阻塞性肺疾病、睡眠呼吸暂停综合征、肺癌、肺间质纤维化、结核、支气管扩张、囊性纤维化等多种呼吸系统疾病.本文把握国外相关研究的最新进展,重点综述了呼出气冷凝液标本收集、贮存、检测等环节对pH值的影响凶素及其在常见呼吸系统疾病中的临床应用.%The technique of exhaled breath condensate is a new detective means for respiratory tract inflammation.It is more and more extensively applied in clinic because of the quick study abroad recently.However,the detectivc technique of pH is the most extensive one in exhaled breath condensate,and it is widely applied for asthma,chronic obstructive pulmonary disease,sleep apnea syndrome,lung cancer,pulmonary interstitial fibrosis,tuberculosis,bronchiectasis,cystic fibrosis and others.This article grasps the latest progress of relative study,reviews the influential factors of pH in exhaled breath condensate,including specimen collection,storage and detection,and the clinical application in the common diseases of respiratory system.

  1. The expression and significance of Th1/Th2 cell factor in the pre-eclampsia%Th1/Th2细胞因子在子痫前期的表达变化及意义

    Institute of Scientific and Technical Information of China (English)

    龚护民; 施蕾

    2011-01-01

    目的:研究Th1/Th2细胞因子在子痫前期的表达变化,并分析其与子痫前期之间的关系.方法:随机搜集2008年3月~2009年10月住院孕妇共60例,其中20例为正常妊娠妇女(对照组),40例为子痫前期患者(子痫前期组,轻度20例,重度20例),采用ELISA法检测孕妇外周静脉血清中细胞因子IFN-γ、IL-4水平.结果:轻度子痫前期组、重度子痫前期组IFN-γ表达水平高于对照组(P<0.01),重度子痫前期组IFN-γ表达水平高于轻度组(P<0.01);轻度子痫前期组、重度子痫前期组IL-4表达水平低于对照组(P<0.01),重度子痫前期组IL-4表达水平低于轻度组(P<0.01).IFN-γ与IL-4水平呈负相关(γ=-0.74,P<0.05);IFN-γ与胎盘重量呈负相关(γ=-0.58,P<0.05);IFN-γ与新生儿体重呈负相关(γ=-0.83,P<0.05);IL-4与胎盘重量呈正相关(γ=0.78,P<0.05);IL-4与新生儿体重呈正相关(γ=0.63,P<0.05).结论:Th1/Th2细胞因子失衡,可能与子痫前期的发生有关.%Objective: To investigate the expression and change of Th1/Th2 cell factor in the pre -eclampsia , and analyze the relationship between cell factor and pre - eclampsia.Methods: 60 hospital pregnant women were randomly selected in our hospital from March 2008 to October 2009, Of which 20 cases were normal pregnant women ( control group), and 40 cases were the pre - eclampsia ( pre - eclampsia group, mild by 20 cases and severe by 20 cases).The level of Th1 -type cytokines IFN-γ, Th2 -type cytokines IL-4 were detected by enzyme- linked immunosorbent assay.Results: The expression of IFN -γin the mild pre -eclampsia group and severe pre -eclampsia group was higher than in the control group ( P < 0.01 ); the expression of IFN - γin the severe pre - eclampsia group was higher than in the mild pre - eclampsia group ( P <0.01 ); the expression of IL - 4 in the mild pre - eclampsia group and severe pre - eclampsia group was lower than in the control group ( P <0.01 ); the

  2. Engineering Significant of Swelling Soils

    Directory of Open Access Journals (Sweden)

    Behzad Kalantari

    2012-08-01

    Full Text Available This study describes some of the most important swelling characters of expansive soils when used as foundation materials to support various types of civil engineering structures. Expansive soils are considered among difficult foundation materials and expand upon wetting and shrink upon losing moisture. They are considered problematic soils for architectural and civil engineers. These types of soils may cause minor to major structural damages to pavements as well as buildings. It is therefore essential to detect swelling soils from non-problematic foundation soils before any civil engineering projects are constructed over or adjacent to them. The study begins with definition of expansive soils and shows its distributions in the world as well as the basic causes for swelling potential that these type of soils poses. It is also shown that, the most probable depth of expansion to check for possible swelling potential for swelling soils is soil’s active zone. This zone is the most upper depth of expansive soil and it may extend up to 20 ft. (6 m below ground level. The moisture content of soil through active zone varies during different seasons while in lower part of expansive soil the moisture content stays constant during hot and cold season. Among various methods to check for swelling potential, plastic index and liquid limits are two most crucial factors, as these factors tend to increase, the swelling potential increase as well.

  3. Creating Significant Learning Experiences across Disciplines

    Science.gov (United States)

    Levine, Laura E.; Fallahi, Carolyn R.; Nicoll-Senft, Joan M.; Tessier, Jack T.; Watson, Cheryl L.; Wood, Rebecca M.

    2008-01-01

    The purpose of this study was to use Fink's (2003) taxonomy of significant learning to redesign courses and assess student learning. Significant improvements were found across the semester for students in the six courses, but there were differences in which taxa showed improvement in each course. The meta-analysis showed significant, positive…

  4. Studies Show Curricular Efficiency Can Be Attained.

    Science.gov (United States)

    Walberg, Herbert J.

    1987-01-01

    Reviews the nine factors contributing to educational productivity, the effectiveness of instructional techniques (mastery learning ranks high and Skinnerian reinforcement has the largest overall effect), and the effects of psychological enviroments on learning. Includes references and a table. (MD)

  5. 基质细胞衍生因子-1在人肝细胞癌中的表达及临床意义%Expression and clinical significance of stromal cell-derived factor-1 in human hepatocellular cancer

    Institute of Scientific and Technical Information of China (English)

    陆健朴; 周栋; 黄志明

    2012-01-01

    目的 观察基质细胞衍生因子-1(SDF-1)在肝细胞肝癌(HCC)中的表达,探讨SDF-1和HCC的临床、病理特点及术后复发的关系.方法 应用免疫组织化学技术检测SDF-1在41例HCC癌组织、癌旁组织和11例正常肝组织中的表达;分析临床、病理资料,并对上述患者进行随访,根据HCC患者术后复发时间进行生存分析.结果 SDF-1在HCC癌组织、癌旁组织及正常肝组织中的表达阳性率分别为68.29%、46.34%、和18.18%(P<0.01).SDF-1表达强度与肿瘤病理分级呈正相关(低分化为66.67%,高分化为43.48%;转移组为52%,未转移组31.25%),差异有统计学意义(P<0.05).SDF-1表达阳性、阴性患者12月内的复发率分别为48.1%和14.3%(P<0.01).结论 SDF-1在HCC中表达上调,SDF-1的高表达在肝癌发生过程中发挥重要作用.SDF-1可作为一种新的判断HCC患者术后复发和预后的指标.%Objective To investigate the expression of stromal cell-derived factor-1 (SDF-1) in hepatocellular cancer (HCC) and its relationship between clinieopathologic features and postoperative recurrences of HCC. Methods Immunohistoetiemistry technique was used to detect the expression of SDF-1 in 4leases of HCC tissues and adjacent tissues and 1 1 cases of normal liver tissues. The clinical and pathologic data were analyzed. Survival analysis was performed according to the recurrent time of postoperative recurrence. Results The positive expression rates of SDF-1 in HCC tissues and adjacent tissues and normal liver tissues were 68.29%, 46.34% and 18.18%, respectively (P<0.01). The expression intensity of SDF-1 was positive correlated with the pathological grade of tumors (The positive expression rate in poorly differentiated group, well-differentiated group, metastasis group and non-metastasis group were 66.67%, 43.48%, 52% and 31.25% respectively) (P<0.05). The recurrence rate of patients in groups positive or negative for SDF-1 was 48.1% and 14.3% respectively within 12

  6. THE SIGNIFICANCE OF STRESS HORMONES IN PSORIASIS

    Directory of Open Access Journals (Sweden)

    F Z Zangeneh

    2008-12-01

    Full Text Available "nPsoriasis is a chronic, non-contagious skin condition characterized by inflamed and scaly lesions of skin. Whilst the pathogenesis of psoriasis is not known, psychological stress has been implicated as a potential trigger in the onset and exacerbation of the condition. Psychiatric and psychological factors play an important role in at least 30% of dermatologic disorder and pathophysiologic link between psychological stress (PS and disease expression remains unclear. Recent studies demonstrated PS-induced alterations in permeability barrier homeostasis, mediated by increased endogenous glucocorticoids. As activation of the hypothalamic pituitary adrenal axis (HPA is critical to a successful stress response, we investigated this in patients with psoriasis. This study was performed on 55 patients (40 females and 15 males visited our clinic for treatment of psoriasis in pharmacology department. We measured the rate of activation of HPA by hormonal changes. These patients displayed higher fasting blood sugar (FBS, epinephrine (Ep, adrenocorticotropin hormone (ACTH, aldosterone, prolactin, growth hormone and estradiol hormones value but diminished cortisol and corticotropin releasing factor (CRF. These results show that HPA and psychoneuroendocrine hormones have a significant role in psoriasis.

  7. Expressions of Wnt-signaling upstream and downstream factors in liver cancer tissue and their clinical significance%肝癌组织中Wnt通路上下游因子的表达及其临床意义

    Institute of Scientific and Technical Information of China (English)

    胡曼; 曾维政; 蒋明德; 吴晓玲

    2013-01-01

    目的 探讨Wnt通路Wif-1、C-myc、Cycling-D1mRNA及蛋白在正常肝组织、肝硬化及肝癌组织中的表达及其与肝癌临床病理参数的相关性.方法 运用免疫组织化学SP法与原位杂交技术检测100份肝癌组织、50份肝硬化组织、50例正常肝组织中Wif-1、C-myc、Cycling-D1蛋白及mRNA的表达,并测定肝癌组织中的Wif-1、C-myc和Cycling-D1的阳性率、敏感性及特异性.结果 肝硬化与肝癌组织中Wif-1 mRNA及蛋白的阳性细胞表达率明显低于正常肝组织(P<0.05),C-myc和Cycling-D1mRNA及蛋白的阳性细胞表达率明显高于正常肝组织(P<0.05).肝癌组织中Wif-l、C-myc和Cycling-D1 mRNA及蛋白的阳性表达均与HBV分级、TNM分期及β-catenin异质表达显著相关(P<0.05).Wif-1、C-myc和Cycling-D1mRNA及蛋白在肝癌组织中的表达呈正相关(rWff-1=0.892,rCmyc=0.354,rcyding-D1=0.378).肝癌组织中mRNA及蛋白的敏感性:Wif-1:9.09%、11.11%,C-myc:91.38%、89.66%,Cycling-D1:91.67%、82.5%,特异性:Wif-1:97.75%、95.6%,C-myc:82.5%、85.71%,Cycling-D1:92.86%、81.82%.结论 肝癌的侵润和β-catenin的异质表达均与Wif-1下降,C-myc及Cycling-D1上调相关.%Objective To investigate the expressions of the mRNA and protein of Wif-1, C-myc and Cycling-Di of Wnt signal pathway in normal liver, cirrhosis and hepatocellular carcinoma tissues as well as their relationship to clinical pathological parameters in hepatocellular carcinoma (HCC). Methods The expressions of Wif-1, C-myc and Cycling-D1 mRNAs and proteins in 100 samples of HCC, 50 samples of liver cirrhosis and 50 samples of normal liver tissue were determined by immunohistochemical assay and in situ hybridization. In addition, the positive rates, sensitivity and specificity of Wif-1, C-myc and Cycling-D1 in HCC were determined. Results The expression rates of Wif-1 mRNA and protein in liver cirrhosis and HCC tissues were significantly lower, while those of C-myc and

  8. MRI shows clodronate-liposomes attenuating liverinjuryinratswithsevereacutepancreatitis

    Institute of Scientific and Technical Information of China (English)

    Jian-Xin Zhang; Sheng-Chun Dang; Yong Zhang; Xin Sha; Li-Rong Zhang; Chuan-She Wei; Min Chen; De-Li Jiang

    2010-01-01

    BACKGROUND: Studies have revealed that macrophages play an important role in the development of severe acute pancreatitis (SAP). Activated macrophages can lead to a systemic inlfammatory response, induce lipid peroxidation, impair membrane structure, result in injury to the liver and the other extrahepatic organs, and eventually result in multiple organ dysfunction syndrome by promoting excessive secretion of cytokines. Liver injury can further aggravate the systemic inlfammatory response and increase mortality by affecting the metabolism of toxins and the release of excessive inlfammatory mediators. Clodronate is a synthetic bisphosphonate, which is often used for treating bone changes caused by osteoporosis and other factors. In the current study, we created liposomes containing superparamagnetic iron oxide particles (SPIOs) for macrophage labeling and magnetic resonance imaging, using a novel method that can bind the clodronate to induce apoptosis and deplete macrophages. METHODS: Superparamagnetic Fe3O4 nanoparticles were prepared by chemical coprecipitation. SPIO-containing liposomes and SPIO-clodronate-containing liposomes were prepared by the thin iflm method. SAP models were prepared by injection of sodium taurocholate (2 ml/kg body weight) into the subcapsular space of the pancreas. Sprague-Dawley rats were randomly divided into a control group, a SAP plus SPIO-liposome group, and a SAP plus SPIO-clodronate-containing group. Two and six hours after SAP models were available, T2-weighted MRI scans (in the same plane) of the livers of rats in each group were performed. At the end of the scans, 2 ml of blood was taken from the superior mesenteric vein to measure the levels of serum amylase, ALT, AST, TNF-α, and IL-6. Pathological changes in the liver and pancreas were assessed. RESULTS: Transmission electron microscopy showed that the liposomes had a uniform size. No pathological changes in the pancreata of rats in the control group were noted. The

  9. 干细胞转录因子Foxd3在宫颈癌组织中的临床表达%Expression and significance of stem cell transfer Factor - Foxd3 in cervical carcinoma

    Institute of Scientific and Technical Information of China (English)

    王旭; 张阳

    2012-01-01

    目的:探讨干细胞转录因子Foxd3在宫颈癌组织中的表达情况及意义.方法:采用荧光实时定量(RT - PCR)方法检测22例宫颈癌组织和12例正常宫颈组织中Foxd3 mRNA的表达水平.采用组织芯片及免疫组织化学技术检测Foxd3的蛋白表达.结果:Foxd3 mRNA在宫颈癌组织与正常组织中均有表达,宫颈癌组织中表达水平显著高于正常组.Foxd3蛋白在正常组织中没有检测到,在宫颈癌中表达水平为90.9%,在宫颈癌组织中的表达水平显著高于正常组织.结论:宫颈癌组织中存在着Foxd3蛋白,可以作为宫颈癌治疗的一个新靶点.%Objective: To study the expression of Foxd3 in cervical carcinoma and its significance. Methods: Reverse transcription PCR ( RT - PCR) was employed to detect Foxd3 mRNA in 22 cases of cervical carcinoma and 12 cases of normal cervix tissues, and tissue array and immunohistochemistry were used to analyze Foxd3 protein expression in cervical carcinoma and normal tissue. Results: The mRNA level of Foxd3 was higher in cervical carcinoma than that in normal cervix, and most of cervical carcinoma 90. 9% than normal cervix expressed Foxd3 protein. Conclusion: There is Foxd3 in cervical carcinoma tissues, it may be a therapeutic target of cervical carcinoma

  10. 组织因子及其抑制物在妊娠期高血压疾病患者胎盘中的表达及临床意义%Expression and clinical significance of placental tissue factor and tissue factor pathway inhibitor in pregnant women with hypertensive disorders

    Institute of Scientific and Technical Information of China (English)

    王晓丹; 韩翠欣; 班玲; 刘雅丽; 剧蕴慧; 梁霞; 田甜

    2016-01-01

    目的:探讨妊娠期高血压疾病患者胎盘组织中组织因子( TF)及其抑制物———组织因子途径抑制物( TFPI)的表达变化,探讨妊娠期高血压疾病的发生机制。方法采用免疫组化法检测妊娠期高血压患者、子痫前期患者及正常妊娠妇女胎盘组织中TF和TFPI的水平。结果与正常妊娠组相比,妊娠期高血压组胎盘表达TF有升高趋势,但差异无统计学意义( P>0.05);与正常妊娠组和妊娠期高血压组相比,子痫前期组胎盘表达TF明显升高,差异有统计学意义( P<0.05)。妊娠期高血压组与正常妊娠组相比,TFPI表达有下降趋势,但差异无统计学意义;子痫前期组胎盘表达TFPI明显下降,与正常妊娠组和妊娠期高血压组相比差异有统计学意义(P<0.05)。结论 TF、TFPI值的变化及其比例失调导致了胎盘内凝血功能的异常,可能与妊娠期高血压疾病的发生、发展有关。%Objective It is to explore whether hypertensive disorders complicating pregnancy was associated with changes of the maternal placental concentrations as well as its inhibitor-tissue factor pathway inhibitor (TFPI).Methods Placental tissue factor( TF) and TFPI expression from gestational hypertension patients, preeclampsia patients and healthy pregnant controls were determined by immunohystochemistry.Results Comparing to healthy pregnant women,TF placental levels in gestational hypertension patients increased but had no difference.TF placental levels were significantly higher in preeclampsia patients than that in gestational hypertension patients and the healthy controls.Comparing to gestational hypertension patients and healthy pregnant women, TFPI placental levels in preeclampsia patients were significantly lower,but the difference between the gestational hypertension patients and the healthy controls was not significantly.Conclusion The changes of plasma TF and TF

  11. 食管癌血管形成相关因子的表达及意义%Expression of Transcription Factor Correlated with Angiogenesis and Their Clinical Significance in Esophageal Carcinoma

    Institute of Scientific and Technical Information of China (English)

    许林平; 庞雅青; 买玲

    2011-01-01

    Objective To investigate the expression of NF-κBVEGF-CMVD and their relationships with esophageal carcinoma of different constitution. Methods Envision immunohistochemical method was used to detect the expression of NF-κB, VEGF-C and MVD in 47 cases of squamous cell esophageal carcinoma ,41 cases of the adenocarcinoma of esophagus and 34 cases of the small cell carcinoma of the esophagus. Results NF-κB positive rate was 59. 57% in squamous cell carcinoma of esophagus,and in adenocarcinoma of esophagus and small cell carcinoma of the esophagus, the rates were 36. 59% and 67. 65 % (P <0. 05),respectively. The positive rates of VEGF-C were 65. 96% 、85. 37% 、79. 41% (P>0. 05), respectively. Conclusion The positive rate of VEGF-C in adenocarcinoma of esophagus was higher than that in squamous cell carcinoma of esophagus and small cell carcinoma of the esophagus. But the NF-κB positive rate was the highest in small cell carcinoma of the esophagus. MVD results showed no difference due to possible different mechanism of angiogenesis. Further investigation will be necessary in order to understand the concrete mechanism.%目的 探讨不同组织类型食管癌中NF-KB、VEGF-C、MVD的表达及与临床特征的关系.方法 采用免疫组织化学二步法检测47例食管鳞癌,41例食管腺癌及34例食管小细胞癌中NF-icB,VEGF-C的表达情况,并对微血管密度(MVD)进行计数.采用RT-PCR检测50例食管癌及20例正常食管组织相关基因.结果 NF-KB在食管鳞癌、腺癌和小细胞癌的阳性表达率分别为59.57%、36.59%和67.65 % (P<0.05);VEGF-C在食管鳞癌、腺癌和小细胞癌阳性表达率为65.96%、85.37%和79.41%(P>0.05).结论 VEGF-C和NF-KB在食管癌发生发展中起着一定的作用,VEGF-C在食管腺癌中表达较高,NF-KB食管小细胞癌中表达较高.MVD在三者之间无差异.食管鳞癌、腺癌和小细胞癌形成微血管的机制可能稍有不同,深入研究其具体的形成

  12. Clinical significance of serum transforming growth factor-β,tumor necrosis factor-α and interferon-γlevels in patients with pre-eclampsia%子痫前期患者血清转化生长因子β、肿瘤坏死因子α和干扰素γ水平变化及其临床意义

    Institute of Scientific and Technical Information of China (English)

    朱卓青; 黄鹰; 杨伶俐; 程妤奋; 古航; 倪鑫

    2015-01-01

    目的:探讨子痫前期患者血清转化生长因子β( TGF-β)、肿瘤坏死因子α( TNF-α)和干扰素γ( IFN-γ)水平变化及其临床意义。方法选取子痫前期产妇32例(轻度子痫前期组22例、重度子痫前期组10例)、妊娠期高血压疾病30例(妊娠期高血压疾病组)和正常产妇30例(正常产妇组)做为研究对象。检测分娩前血清TGF-β、TNF-α和IFN-γ浓度,分析不良妊娠结局与上述指标的关系。结果重度子痫前期组血清TGF-β[(90.4±23.4)μg/L]、TNF-α[(84.5±13.6)μg/L]和IFN-γ[(146.5±13.4)μg/L]水平显著高于正常产妇组[(11.3±3.7)μg/L、(5.6 ± 1.2)μg/L、(82.5±19.4)μg/L]、妊娠期高血压疾病组[(35.3±8.4)μg/L、(10.4±2.9)μg/L 、(96.4 ± 15.8)μg/L]、轻度子痫前期组[(76.5±15.4)μg/L、(26.5± 3.2)μg/L、(120.4±20.5)μg/L],差异有统计学意义(F 值分别为11.363、15.982、7.431,P均﹤0.001);轻度子痫前期组血清TGF-β、TNF-α和IFN-γ水平显著高于正常产妇组、妊娠期高血压疾病组( P均﹤0.05)。TGF-β、TNF-α、IFN-γ高表达患者早产的发生率显著高于低表达患者(7例与2例,χ2=4.037,P=0.044)。TGF-β、TNF-α高表达患者胎儿生长受限的发生率显著高于低表达患者(6例与1例,χ2=4.969,P=0.025)。结论检测妊娠孕妇血清各项细胞因子的变化可在一定程度上评估孕妇子痫前期的严重程度及对不良妊娠结局进行预测。%Objective To investigate the clinical significance of serum transforming growth factor-β( TGF-β),tumor necrosis factor-α( TNF-α) and interferon-γ( IFN-γ) levels in patients with pre-eclampsia. Methods Thirty-two cases of maternal pre-eclampsia( 22 cases were mild pre-eclampsia,10 cases were severe pre-eclampsia),30 cases of gestational hypertension and 30 cases of normal mothers were selected as our

  13. Oilsands for the USA : while environmental groups ask for a shutdown, new study shows significant resulting economic benefits in America

    Energy Technology Data Exchange (ETDEWEB)

    Cook, D.L.

    2010-02-15

    The United States is beginning to appreciate the value of having massive oil sands resources located in relatively close proximity to their northern border. This article discussed a recent study conducted by the Canadian Energy Research Institute (CERI) to assess the impact of Canada's oil sands development on the economy of the United States. The study forecasted that the demand for oil sands-related goods and services from American companies will continue to increase as the industry expands. The top national-level goods and services impacts will be derived from increases in manufacturing; finance; insurance; real estate; and professional, scientific, and technical services. Accommodation and food services in the United States will also benefit from the growth of the oil sands industry. The United States may not risk pushing ahead with strict carbon-cutting legislation targeting the oil sands when policy-makers consider the potential impacts of Canada selling its resources to China. 1 fig.

  14. Robust Simulations and Significant Separations

    CERN Document Server

    Fortnow, Lance

    2010-01-01

    We define and study a new notion of "robust simulations" between complexity classes which is intermediate between the traditional notions of infinitely-often and almost-everywhere, as well as a corresponding notion of "significant separations". A language L has a robust simulation in a complexity class C if there is a language in C which agrees with L on arbitrarily large polynomial stretches of input lengths. There is a significant separation of L from C if there is no robust simulation of L in C. The new notion of simulation is a cleaner and more natural notion of simulation than the infinitely-often notion. We show that various implications in complexity theory such as the collapse of PH if NP = P and the Karp-Lipton theorem have analogues for robust simulations. We then use these results to prove that most known separations in complexity theory, such as hierarchy theorems, fixed polynomial circuit lower bounds, time-space tradeoffs, and the theorems of Allender and Williams, can be strengthened to signifi...

  15. Significance of biofilms in dentistry.

    Science.gov (United States)

    Wróblewska, Marta; Strużycka, Izabela; Mierzwińska-Nastalska, Elżbieta

    2015-01-01

    In the past decades significant scientific progress has taken place in the knowledge about biofilms. They constitute multilayer conglomerates of bacteria and fungi, surrounded by carbohydrates which they produce, as well as substances derived from saliva and gingival fluid. Modern techniques showed significant diversity of the biofilm environment and a system of microbial communication (quorum sensing), enhancing their survival. At present it is believed that the majority of infections, particularly chronic with exacerbations, are a result of biofilm formation, particularly in the presence of biomaterials. It should be emphasised that penetration of antibiotics and other antimicrobial agents into deeper layers of a biofilm is poor, causing therapeutic problems and necessitating sometimes removal of the implant or prosthesis. Biofilms play an increasing role in dentistry as a result of more and more broad use in dental practice of plastic and implantable materials. Biofilms are produced on the surfaces of teeth as dental plaque, in the para-nasal sinuses, on prostheses, dental implants, as well as in waterlines of a dental unit, constituting a particular risk for severely immunocompromised patients. New methods of therapy and prevention of infections linked to biofilms are under development.

  16. MICROENTERPRISES AND SIGNIFICANT RISK FACTORS IN LOAN PROCESS

    National Research Council Canada - National Science Library

    Jaroslav Belás; Sergej Vojtovic; Aleksandr Kljucnikov

    2016-01-01

      Microenterprises - small companies with their own characteristic features - are a major component of the economic system, which brings positive effects on employment, increases the supply of goods...

  17. Testis cancer. Ichthyosis constitutes a significant risk factor

    DEFF Research Database (Denmark)

    Lykkesfeldt, G; Bennett, P; Lykkesfeldt, A E

    1991-01-01

    Testis cancer and ichthyosis are both relatively rare diseases. Hence the finding of six individuals with both these conditions in a small population with testicular cancer is highly conspicuous and indicates some kind of connection among such persons. Despite the identical clinical appearances...... of their ichthyoses, three of the ichthyotic subjects had no measurable activity of the enzyme, steroid sulfatase (STS) in leucocytes, a distinct characteristic of recessive X-linked ichthyosis (RXLI). However, the remaining three subjects had normal STS activity, a strong indicator of autosomal dominant ichthyosis...... (ADI). The STS activity in patients with testicular cancer who do not have ichthyosis (N = 30) was also within the normal range. The patients with testicular cancer with no skin disease had elevated serum levels of 4-androstenedione (4-AD), follicle stimulating hormone (FSH), and luteinizing hormone...

  18. Drug and Alcohol Use -- A Significant Risk Factor for HIV

    Science.gov (United States)

    ... Anabolic) Synthetic Cannabinoids (K2/Spice) Synthetic Cathinones (Bath Salts) Tobacco/Nicotine Other Drugs Related Topics Addiction Science Adolescent Brain Comorbidity College-Age & Young Adults ...

  19. Fabrics China Creation