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Sample records for factor vii recombinante

  1. Uso de fator VII recombinante ativado para tratamento e profilaxia de grandes sangramentos Use of recombinant activated factor VII for treatment and prophylaxis of major bleeding

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    Flávio Augusto Henriques Vince

    2009-09-01

    Full Text Available INTRODUÇÃO: O fator VII recombinante ativado (rFVIIa é uma proteína produzida por engenharia genética, cuja estrutura é muito semelhante à do fator VII intrínseco ativado (FVII. Sua ação se baseia no conhecimento do mecanismo de coagulação in vivo agindo na ativação direta do fator X resultando em formação independente de trombina no local da lesão e contribuindo dessa forma para formação de coágulo estável de fibrina sem a ação dos fatores VIII e IX. MÉTODOS: Foi realizada ampla revisão da literatura com o objetivo de determinar os achados recentes relacionados ao uso de fator VII recombinante ativado em pacientes com sangramento severo. RESULTADOS: Constatou-se que o uso de rFVIIa foi iniciado na década de 80 para profilaxia e tratamento de sangramento em pacientes com antecedente de hemofilia A ou B com inibidores de fator VIII e IX, deficiência de fator VII e trombastenia de Glanzmann refratários à reposição plaquetária. Em 1999 seu uso foi ampliado para outras situações clínicas e dessa forma, começaram a ser publicados diversos trabalhos mostrando a eficácia do rFVIIa como agente pró-hemostático em paciente com outras coagulopatias ou pacientes previamente hígidos com história de sangramento agudo de grande monta. Trauma é a principal causa de mortalidade no mundo sendo sangramento incontrolado o principal desafio no atendimento a estes pacientes. É comum a associação de trauma com coagulopatia, necessitando em algumas situações de terapia específica para o tratamento da mesma. Neste momento a terapia adjuvante com rFVIIa deve ser considerada. Outras causas comuns de sangramento são as operações cardíacas e ginecológicas/ obstétricas e doenças envolvendo o fígado. A coagulopatia nesses casos ocorre por deficiência dos fatores dependentes de vitamina K, sendo o FVII o com menor meia-vida. CONCLUSÃO: O uso de rFVIIa tem sido sugerido como opção terapêutica promissora para esses

  2. Tratamiento de sangrado postoperatorio en cirugía cardiaca con Factor VII Recombinante

    OpenAIRE

    Burbano Paredes, Claudia C.; Rincón Riveros, Jose Domingo; Rincón Franco, Ismael

    2010-01-01

    El sangrado posterior a cirugía cardiaca es una complicación importante dada la alta morbimortalidad asociada. La hemotransfusión es la terapia mandatoria, pero la administración masiva de hemoderivados también es un factor de riesgo independiente de morbimortalidad. El factor VIIr se ha propuesto para disminuir las trasfusiones y controlar sangrado. El propósito de este estudio fue determinar si el factor VIIr es una herramienta útil para disminuir el consumo de hemoderivados en sangrado pos...

  3. Factor VII deficiency

    Science.gov (United States)

    ... if one or more of these factors are missing or are not functioning like they should. Factor VII is one such coagulation factor. Factor VII deficiency runs in families (inherited) and is very rare. Both parents must ...

  4. Genetics Home Reference: factor VII deficiency

    Science.gov (United States)

    ... VII deficiency , is caused by mutations in the F7 gene, which provides instructions for making a protein ... about the gene associated with factor VII deficiency F7 Related Information What is a gene? What is ...

  5. Congenital deficiency of factor VII.

    Science.gov (United States)

    Sikka, M; Gomber, S; Madan, N; Rusia, U; Sharma, S

    1996-01-01

    A case of congenital factor VII deficiency in a five-year-old child is reported. The patient, born of a non-consanguineous marriage, presented with repeated bouts of epistaxis since childhood. The prothrombin time (PT) was markedly prolonged with a normal bleeding time (BT), partial thromboplastin time with Kaolin (PTTK) and platelet count. The patient has been on follow up for the last four years and is doing apparently well.

  6. [ELISA method for the determination of factor VII antigen].

    Science.gov (United States)

    Jorquera, J I; Aznar, J A; Monteagudo, J; Montoro, J M; Casaña, P; Pascual, I; Bañuls, E; Curats, R; Llopis, F

    1989-12-01

    The low plasma concentration of clotting factor VII makes it difficult to assay its antigenic fraction by the conventional methods of precipitation with specific antigens. Simple and peroxidase-conjugated antisera are currently available from commercial sources, thus allowing one to determine F VII:Ag by enzyme immunoassay. An ELISA method has been developed in this laboratory which provides sensitivity limits about 0.1% of the plasma concentration of F VII and correlates significantly with its functional activity (r = 0.603, n = 44, p less than 0.001). This technique can be highly helpful in characterising molecular variants of F VII, as well as in detecting acquired deficiencies of this factor.

  7. Plasma factor VII-activating protease is increased by oral contraceptives and induces factor VII activation in-vivo

    DEFF Research Database (Denmark)

    Sidelmann, Johannes J; Skouby, Sven O; Kluft, Cornelis

    2011-01-01

    Oral contraceptive (OC) use influences the hemostatic system significantly and is a risk factor for development of cardiovascular disease. Factor VII-activating protease (FSAP) has potential effects on hemostasis. The 1601GA genotype of the 1601G/A polymorphism in the FSAP gene expresses a FSAP...... alloenzyme with reduced pro-fibrinolytic activity. Presently, we address whether OC use and OC formulation affect FSAP measures in human blood. Healthy women (n=588) were allocated to six cycles of OCs with estrogen contents of 20µg (n=158), 30µg (n=284), 35µg (n=79) or 50µg (n=67) combined with various...... progestins. FSAP genotypes, FSAP and factor VII (FVII) plasma measures were assessed at baseline and after 6 cycles of OC. The 1601GA genotype was present in 49 (8.3%) of the women and was associated with significantly reduced levels of FSAP (P=0.001). OC use increased FSAP antigen by 25% and FSAP activity...

  8. Papel del factor de crecimiento epidérmico recombinante humano “Heberprot-P” en un paciente con Lepra Multibacilar

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    Wilver Velázquez Chacón

    2014-08-01

    Full Text Available Se presenta el caso de un paciente masculino de 59 años de edad, tratado por vías convencionales a causa de lesiones ulcerativas de miembros inferiores, de más de siete años de evolución, sin diagnóstico y tratamiento. La evolución ulterior permitió reconocer la aparición subsecuente de lepra multibacilar. La basiloscopía fue positiva, indicando el diagnóstico de las lesiones ulcerativas, que se trataron con el uso de factor de crecimiento epidérmico recombinante humano en su formulación Heberprot-P, permitiendo la granulación de las lesiones en toda su extensión en un plazo corto, de 16 días, sin que se presentaran eventos adversos graves. Su resultado final fue satisfactorio

  9. Coagulation factor VIIa: prohemostatic drug and biomarker for thrombosis

    NARCIS (Netherlands)

    Schut, Anne Marieke

    2016-01-01

    Stollingsfactor VIIa: prohemostatisch medicijn en biomarker voor trombose Fysiologische activatie van de bloedstolling begint met de activatie van stollings factor VII tot factor VIIa. Een recombinante vorm van factor VIIa (rFVIIa) is ontwikkeld als medicijn om bloedstolling te stimuleren in patiënt

  10. Plasma factor VII-activating protease is increased by oral contraceptives and induces factor VII activation in-vivo

    DEFF Research Database (Denmark)

    Sidelmann, Johannes Jakobsen; Skouby, Sven O.; Kluft, Cornelis

    2011-01-01

    Oral contraceptive (OC) use influences the hemostatic system significantly and is a risk factor for development of cardiovascular disease. Factor VII-activating protease (FSAP) has potential effects on hemostasis. The 1601GA genotype of the 1601G/A polymorphism in the FSAP gene expresses a FSAP...... alloenzyme with reduced pro-fibrinolytic activity. Presently, we address whether OC use and OC formulation affect FSAP measures in human blood. Healthy women (n=588) were allocated to six cycles of OCs with estrogen contents of 20μg (n=158), 30μg (n=284), 35μg (n=79) or 50μg (n=67) combined with various...... progestins. FSAP genotypes, FSAP and factor VII (FVII) plasma measures were assessed at baseline and after 6 cycles of OC. The 1601GA genotype was present in 49 (8.3%) of the women and was associated with significantly reduced levels of FSAP (P≤0.001). OC use increased FSAP antigen by 25% and FSAP activity...

  11. Synthesis and characterization of 18F-labeled active site inhibited factor VII (ASIS)

    DEFF Research Database (Denmark)

    Erlandsson, Maria; Nielsen, Carsten Haagen; Jeppesen, Troels Elmer

    2015-01-01

    Activated factor VII blocked in the active site with Phe-Phe-Arg-chloromethyl ketone (active site inhibited factor VII (ASIS)) is a 50-kDa protein that binds with high affinity to its receptor, tissue factor (TF). TF is a transmembrane glycoprotein that plays an important role in, for example, th...

  12. Coagulation factor VII, serum-triglycerides and the R/Q353 polymorphism: Differences between older men and women

    NARCIS (Netherlands)

    Mennen, L.I.; Maat, M.P.M. de; Schouten, E.G.; Kluft, C.; Jong, P.T.V.M. de; Hofman, A.; Grobbee, D.E.

    1997-01-01

    Coagulation factor VII activity (FVII:C) is a risk indicator for cardiovascular disease. It is related to serum-triglycerides and the R/Q353 polymorphism (alleles R and Q) in the gene coding for factor VII is strongly associated with factor VU[. The association of serum-triglycerides with factor VII

  13. Coagulation factor VII, serum-triglycerides and the R/Q353 polymorphism: Differences between older men and women

    NARCIS (Netherlands)

    Mennen, L.I.; Maat, M.P.M. de; Schouten, E.G.; Kluft, C.; Jong, P.T.V.M. de; Hofman, A.; Grobbee, D.E.

    1997-01-01

    Coagulation factor VII activity (FVII:C) is a risk indicator for cardiovascular disease. It is related to serum-triglycerides and the R/Q353 polymorphism (alleles R and Q) in the gene coding for factor VII is strongly associated with factor VU[. The association of serum-triglycerides with factor VII

  14. Factor VII deficiency impairs cutaneous wound healing in mice.

    Science.gov (United States)

    Xu, Zhi; Xu, Haifeng; Ploplis, Victoria A; Castellino, Francis J

    2010-01-01

    Skin keratinocytes express tissue factor (TF) and are highly associated with skin wound healing. Although it has been demonstrated that perivascular TF expression in granulation tissue formed after dermal injury is downregulated during healing, studies of the mechanism of factor (F) VII, a TF ligand, in skin wound healing are lacking. We reported the use of a dermal punch model to demonstrate that low-expressing FVII mice (approximately 1% of wild type [WT]) exhibited impaired skin wound healing compared with WT controls. These low-FVII mice showed defective reepithelialization and reduced inflammatory cell infiltration at wound sites. This attenuated reepithelialization was associated with diminished expression of the transcription factor early growth response 1 (Egr-1). In vitro, Egr-1 was shown to be essential for the FVIIa-induced regulation of keratinocyte migration and inflammation. Both Egr-1 upregulation and downstream inflammatory cytokine appearance in keratinocytes depended on FVIIa/TF/protease-activated receptor 2 (PAR-2)-induced signaling and did not require subsequent generation of FXa and thrombin. The participation of Egr-1 in FVIIa-mediated regulation of keratinocyte function was confirmed by use of Egr-1-deficient mice, wherein a significant delay in skin wound healing after injury was observed, relative to WT mice. The results from these studies demonstrate an in vivo mechanistic relationship between FVIIa, Egr-1 and the inflammatory response in keratinocyte function during the wound healing process.

  15. Factor VII activating protease. Single nucleotide polymorphisms light the way.

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    Kanse, S M; Etscheid, M

    2011-08-01

    Factor VII activating protease (FSAP) is a circulating serine protease with high homology to fibrinolytic enzymes. A role in the regulation of coagulation and fibrinolysis is suspected based on in vitro studies demonstrating activation of FVII or pro-urokinase plasminogen activator (uPA). However, considering the paucity of any studies in animal models or any correlative studies in humans the role of FSAP in haemostasis remains unclear. In relation to vascular remodeling processes or inflammation it has been convincingly shown that FSAP interacts with growth factors as well as protease activated receptors (PAR). Against this sparse background there are a plethora of studies which have investigated the linkage of single nucleotide polymorphisms (SNP) in the FSAP gene (HABP2) to various diseases. The G534E SNP of FSAP is associated with a low proteolytic activity due to an amino acid exchange in the protease domain. This and other SNPs have been linked to carotid stenosis, stroke as well as thrombosis in the elderly and plaque calcification. These SNP analyses indicate an important role for FSAP in the regulation of the haemostasis system as well as fibroproliferative inflammatory processes.

  16. Recombinant activated factor VII in post partum haemorrhage

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    Navneet Magon

    2013-01-01

    Full Text Available Post-partum haemorrhage (PPH is a life-threatening obstetric complication and the leading cause of maternal death. Any bleeding that results in or could result in haemodynamic instability, if untreated, must be considered as PPH. There is no controversy about the need for prevention and treatment of PPH. The keystone of management of PPH entails first, non-invasive and nonsurgical methods and then invasive and surgical methods. However, mortality remains high. Therefore, new advancements in the treatment are most crucial. One such advancement has been the use of recombinant activated factor VII (rFVIIa in PPH. First used 12 years back in PPH, this universal haemostatic agent has been effectively used in controlling PPH. The best available indicator of rFVIIa efficacy is the arrest of haemorrhage, which is judged by visual evidence and haemodynamic stabilization. It also reduces costs of therapy and the use of blood components in massive PPH. In cases of intractable PPH with no other obvious indications for hysterectomy, administration of rFVIIa should be considered before surgery. We share our experience in a series of cases of PPH, successfully managed using rFVIIa.

  17. Effects of recombinant activated factor VII on coagulation measured by thromboelastography in liver transplantation

    NARCIS (Netherlands)

    Hendriks, HGD; Meijer, K; de Wolf, JTM; Porte, RJ; Klompmaker, IJ; Lip, H; Slooff, MJH; van der Meer, J

    Besides the conventional laboratory tests, thromboelastography (TEG) is used to monitor hemostasis during liver transplantation. A previous pilot study suggested a beneficial effect of recombinant activated factor VII (rFVIIa) on transfusion requirements in liver transplantation. In the present

  18. Effects of different progestin regimens in hormone replacement therapy on blood coagulation factor VII and tissue factor pathway inhibitor

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Skouby, S O.; Andersen, L F;

    2002-01-01

    BACKGROUND: Long-term hormone replacement therapy (HRT) reduces cardiovascular risk, but an early increased risk was reported in women with coronary heart disease. In such women the arterial intima can express tissue factor, and changes in coagulation factor VII (factor VII) and tissue factor...... after progestin intake. The integrated response, AUC, for TFPI was significantly lower in the HRT groups compared with the reference group. CONCLUSION: The observed changes may increase the early thrombotic risk associated with HRT use. Udgivelsesdato: 2002-Dec...

  19. Topical application of recombinant activated factor VII during cesarean delivery for placenta previa.

    Science.gov (United States)

    Schjoldager, Birgit T B G; Mikkelsen, Emmeli; Lykke, Malene R; Præst, Jørgen; Hvas, Anne-Mette; Heslet, Lars; Secher, Niels J; Salvig, Jannie D; Uldbjerg, Niels

    2017-06-01

    During cesarean delivery in patients with placenta previa, hemorrhaging after removal of the placenta is often challenging. In this condition, the extraordinarily high concentration of tissue factor at the placenta site may constitute a principle of treatment as it activates coagulation very effectively. The presumption, however, is that tissue factor is bound to activated factor VII. We hypothesized that topical application of recombinant activated factor VII at the placenta site reduces bleeding without affecting intravascular coagulation. We included 5 cases with planned cesarean delivery for placenta previa. After removal of the placenta, the surgeon applied a swab soaked in recombinant activated factor VII containing saline (1 mg in 246 mL) to the placenta site for 2 minutes; this treatment was repeated once if the bleeding did not decrease sufficiently. We documented the treatment on video recordings and measured blood loss. Furthermore, we determined hemoglobin concentration, platelet count, international normalized ratio, activated partial thrombin time, fibrinogen (functional), factor VII:clot, and thrombin generation in peripheral blood prior to and 15 minutes after removal of the placenta. We also tested these blood coagulation variables in 5 women with cesarean delivery planned for other reasons. Mann-Whitney test was used for unpaired data. In all 5 cases, the uterotomy was closed under practically dry conditions and the median blood loss was 490 (range 300-800) mL. There were no adverse effects of recombinant activated factor VII and we did not measure factor VII to enter the circulation. Neither did we observe changes in thrombin generation, fibrinogen, activated partial thrombin time, international normalized ratio, and platelet count in the peripheral circulation (all P values >.20). This study indicates that in patients with placenta previa, topical recombinant activated factor VII may diminish bleeding from the placenta site without initiation

  20. Polymorphisms in the genes for coagulation factor II,V,VII in patients undergoing coronary angiography

    Institute of Scientific and Technical Information of China (English)

    徐耕; 金国栋; 傅国胜; 马骥; 单江; 王建安

    2003-01-01

    Objective: To determine whether polymorphisms in the genes for coagulation factor II,V, VII could predispose an individual to increase risk for coronary artery disease (CAD) and/or myocardial infarction (MI) in Chinese. Methods: We screened coagulation factor II(G20210A),V(G1691A),VII (R353Q and HVR4) genotype in 374 patients undergoing coronary angiography by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay. Results: The R353Q and HVR4 genotype of the factor VII distribution was in accordance with Hardy-Weinberg equilibrium. The frequencies of FVII genotype or allele did not show statistically significant differences between CAD group and controls or between male and female. The frequencies of the Q allele and (RQ+QQ) genotype were significantly higher among the CAD patients without myocardial infarction (MI) history than among those with MI history (P<0.05). However, HVR4 polymorphism was not significantly different within groups. We only find one normal control of factorII(G20210A) mutation. No coagulation factor V(G1691A) mutation was found in the CAD patients and controls. Conclusion: The factor II(G20210A),V(G1691A) mutation is absent and may not be a major genetic factor for CAD and/or MI; the Q allele of the R353Q polymorphism of the factor VII gene may be a protective genetic factor against myocardial infarction in Chinese.

  1. Dietary effects on coagulation factor VII vary across genotypes of the R/Q353 polymorphism in elderly people

    NARCIS (Netherlands)

    Mennen, L.I.; Maat, M.P.M. de; Schouten, E.G.; Kluft, C.; Witteman, J.C.M.; Hofman, A.; Grobbee, D.E.

    1998-01-01

    The objective of this study was to evaluate the association of factor VII with dietary factors while also considering the R/Q353 polymorphism. Nutrition is an important determinant of coagulation factor VII, which is also genetically determined by the R/Q353 polymorphism. High levels of coagulation

  2. Prophylaxis in congenital factor VII deficiency: indications, efficacy and safety. Results from the Seven Treatment Evaluation Registry (STER).

    Science.gov (United States)

    Napolitano, Mariasanta; Giansily-Blaizot, Muriel; Dolce, Alberto; Schved, Jean F; Auerswald, Guenter; Ingerslev, Jørgen; Bjerre, Jens; Altisent, Carmen; Charoenkwan, Pimlak; Michaels, Lisa; Chuansumrit, Ampaiwan; Di Minno, Giovanni; Caliskan, Umran; Mariani, Guglielmo

    2013-04-01

    Because of the very short half-life of factor VII, prophylaxis in factor VII deficiency is considered a difficult endeavor. The clinical efficacy and safety of prophylactic regimens, and indications for their use, were evaluated in factor VII-deficient patients in the Seven Treatment Evaluation Registry. Prophylaxis data (38 courses) were analyzed from 34 patients with severe factor VII deficiency (plasma-derived factor VII, and ten received fresh frozen plasma. Prophylactic schedules clustered into "frequent" courses (three times weekly, n=23) and "infrequent" courses (≤ 2 times weekly, n=15). Excluding courses for menorrhagia, "frequent" and "infrequent" courses produced 18/23 (78%) and 5/12 (41%) "excellent" outcomes, respectively; relative risk, 1.88; 95% confidence interval, 0.93-3.79; P=0.079. Long term prophylaxis lasted from 1 to >10 years. No thrombosis or new inhibitors occurred. In conclusion, a subset of patients with factor VII deficiency needed prophylaxis because of severe bleeding. Recombinant activated factor VII schedules based on "frequent" administrations (three times weekly) and a 90 μg/kg total weekly dose were effective. These data provide a rationale for long-term, safe prophylaxis in factor VII deficiency.

  3. Hormone therapy affects plasma measures of factor VII-activating protease in younger postmenopausal women

    DEFF Research Database (Denmark)

    Mathiasen, Jørn Sidelmann; Skouby, S.O.; Vitzthum, F.;

    2010-01-01

    Objectives Current reviews indicate that hormone therapy (HT) has a protective role in coronary heart disease (CHD) in younger postmenopausal women, whereas HT contributes to CHD in older women Factor VII-activating protease (FSAP) is a serine protease that accumulates in unstable atherosclerotic...

  4. Factor VII-activating protease in patients with acute deep venous thrombosis

    DEFF Research Database (Denmark)

    Sidelmann, Johannes J; Vitzthum, Frank; Funding, Eva;

    2008-01-01

    Factor VII-activating protease (FSAP) is involved in haemostasis and inflammation. FSAP cleaves single chain urokinase-type plasminogen activator (scu-PA). The 1601GA genotype of the 1601G/A polymorphism in the FSAP gene leads to the expression of a FSAP variant with reduced ability to activate scu...

  5. Coexistence of Factor VII Deficiency and Hereditary Spastic Paraplegia in Two Siblings

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    Hortensia De la Corte-Rodriguez

    2016-01-01

    Full Text Available We present the case of two patients aged 12 years and 7 years who were referred to our hospital for factor VII deficiency inherited in an autosomal recessive pattern, who had suffered from previous multiple joint haemarthroses. They presented with fine motor symptoms and difficulty in walking. During physical examination we observed neurological symptoms (general hypotonia, muscular hypotrophy, exaggerated tendon reflexes, pes cavus, and spastic gait. Given that the symptoms were not justified by the deficiency of coagulation factor VII and on suspicion of hereditary spastic paraplegia (HSP, tests were carried out. Findings from the tests confirmed the diagnosis of HSP (axonal degeneration of the central motor pathway and pyramidal tracts, further complicated by mixed neuropathy. This disease was also inherited in an autosomal recessive pattern with no direct genetic association with factor VII deficiency. Neurological symptoms had gone unnoticed due to a history of multiple joint haemarthrosis; musculoskeletal examination led to a satisfactory differential diagnosis. Haematological prophylaxis was commenced with rFVIIa at 30 mcg/kg, three days per week. A rehabilitation programme was prescribed so that the patient could remain independent for as long as possible, based on orthosis, physiotherapy, and occupational therapy. Response to treatment is currently satisfactory and no new bleeding has presented. As far as we are aware, the coexistence of these two diseases (factor VII deficiency and HSP has not been previously reported in the literature.

  6. PET Imaging of Tissue Factor in Pancreatic Cancer Using 64Cu-Labeled Active Site-Inhibited Factor VII

    DEFF Research Database (Denmark)

    Nielsen, Carsten H; Jeppesen, Troels E; Kristensen, Lotte K

    2016-01-01

    with advanced stage, increased microvessel density, metastasis, and poor overall survival. Imaging of TF expression is of clinical relevance as a prognostic biomarker and as a companion diagnostic for TF-directed therapies currently under clinical development. Factor VII (FVII) is the natural ligand to TF...

  7. Vena porta thrombosis in patient with inherited factor VII deficiency

    DEFF Research Database (Denmark)

    Klovaite, Jolanta; Friis-Hansen, Lennart Jan; Larsen, Fin S;

    2010-01-01

    with inherited FVII deficiency and chronic vena porta thrombosis. She presented at 32 weeks of gestation with spontaneously increased international normalized ratio, severe thrombocytopenia and very few unspecific symptoms. The extensive examination of the patient revealed cavernous transformation of the portal...... vein with well expressed portosystemic collaterals, heterozygosity for three common polymorphisms in FVII gene, associated with reduction in plasma FVII levels, and no other factors predisposing to thrombosis....

  8. Factor VII activating protease (FSAP) promotes the proteolysis and inhibition of tissue factor pathway inhibitor (TFPI)

    Science.gov (United States)

    Kanse, Sandip M.; Declerck, Paul J.; Ruf, Wolfram; Broze, George; Etscheid, Michael

    2013-01-01

    Objectives Factor VII activating protease (FSAP) activates FVII as well as pro-urokinase and inhibits platelet-derived growth factor-BB, thus regulating haemostasis- and remodeling-associated processes in the vasculature. A genetic variant of FSAP (Marburg I polymorphism) results in low enzymatic activity and is associated with an enhanced risk for carotid stenosis and stroke. We postulate that there are additional substrates for FSAP that will help to explain its role in vascular biology and have searched for such a substrate. Results and Methods Using screening procedures to determine the influence of FSAP on various haemostasis-related processes on endothelial cells we discovered that FSAP inhibited tissue factor pathway inhibitor (TFPI), a major anti-coagulant secreted by these cells. Proteolytic degradation of TFPI by FSAP could also be demonstrated by Western blotting and the exact cleavage sites were determined by N-terminal sequencing. The Marburg I variant of FSAP had a diminished ability to inhibit TFPI. A monoclonal antibody to FSAP, that specifically inhibited FSAP binding to TFPI, reversed the inhibitory effect of FSAP on TFPI. Conclusions The identification of TFPI as a sensitive substrate for FSAP increases our understanding of its role in regulating haemostasis and proliferative remodeling events in the vasculature. PMID:22116096

  9. Effect of individual dietary fatty acids on postprandial activation of blood coagulation factor VII and fibrinolysis in healthy young men

    DEFF Research Database (Denmark)

    Tholstrup, T.; Miller, G.J.; Bysted, Anette

    2003-01-01

    Background: Hypertriglyceridemia may represent a procoagulant state involving disturbances to the hemostatic system. Plasminogen activator inhibitor type 1 (PAI-1) is increased in the presence of hypertriglyceridemia. Free fatty acids (FFAs) in plasma may promote factor VII (FVII) activation...

  10. Factors V and VII anticoagulant activities in the salivary glands of feeding Dermacentor andersoni ticks.

    Science.gov (United States)

    Gordon, J R; Allen, J R

    1991-02-01

    The salivary glands of Dermacentor andersoni ticks possess anticoagulant activities that can alter the clotting time of rabbit whole blood. Salivary gland extracts from female ticks inhibit both the intrinsic and extrinsic coagulation systems, and maximal activities against both pathways occur when the ticks attain about 250 mg feeding weight. These anticoagulants are directed against both coagulation factors V and VII, but they do not affect factors II or X. Despite this salivary anticoagulant activity, heavily tick-infested rabbits suffer no visible alteration of their peripheral blood coagulability and have no detectable circulating fibrin degradation products, suggesting that the ticks do not secrete a factor with fibrinolytic activity.

  11. A high affinity monoclonal antibody recognizing the light chain of human coagulating factor VII.

    Science.gov (United States)

    Sarial, Sheila; Asadi, Farzad; Jeddi-Tehrani, Mahmood; Hadavi, Reza; Bayat, Ali Ahmad; Mahmoudian, Jafar; Taghizadeh-Jahed, Masoud; Shokri, Fazel; Rabbani, Hodjattallah

    2012-12-01

    Factor VII (FVII) is a serine protease-coagulating element responsible for the initiation of an extrinsic pathway of clot formation. Here we generated and characterized a high affinity monoclonal antibody that specifically recognizes human FVII. Recombinant human FVII (rh-FVII) was used for the production of a monoclonal antibody using BALB/c mice. The specificity of the antibody was determined by Western blot using plasma samples from human, mouse, sheep, goat, bovine, rabbit, and rat. Furthermore, the antibody was used to detect transiently expressed rh-FVII in BHK21 cell line using Western blot and sandwich ELISA. A mouse IgG1 (kappa chain) monoclonal antibody clone 1F1-B11 was produced against rh-FVII. The affinity constant (K(aff)) of the antibody was calculated to be 6.4×10(10) M(-1). The antibody could specifically recognize an epitope on the light chain of hFVII, with no reactivity with factor VII from several other animals. In addition, transiently expressed rh-FVII in BHK21 cells was recognized by 1F1-B11. The high affinity as well as the specificity of 1F1-B11 for hFVII will facilitate the affinity purification of hFVII and also production of FVII deficient plasma and minimizes the risk of bovine FVII contamination when fetal bovine serum-supplemented media are used for production and subsequent purification of rh-FVII.

  12. Genotype and phenotype correlation in intracranial hemorrhage in neonatal factor VII deficiency among Thai children

    Directory of Open Access Journals (Sweden)

    Traivaree C

    2017-06-01

    Full Text Available Chanchai Traivaree,1 Chalinee Monsereenusorn,1 Arunotai Meekaewkunchorn,2 Premsak Laoyookhong,3 Saranya Suwansingh,4 Boonchai Boonyawat5 1Division of Hematology/Oncology, Department of Pediatrics, Phramongkutklao Hospital and College of Medicine, 2Division of Hematology/Oncology, Department of Pediatrics, 3Division of Neonatology, Department of Pediatrics, Queen Sirikit National Institute of Child Health, Bangkok, 4Division of Hematology/Oncology, Department of Pediatrics, Chiangrai Prachanukroh Hospital, Chiang Rai, 5Division of Genetics, Department of Pediatrics, Phramongkutklao Hospital and College of Medicine, Bangkok, Thailand Abstract: Congenital factor VII (FVII deficiency is a rare inherited coagulopathy. The clinical manifestations and clinical findings vary widely, ranging from asymptomatic to life-threatening bleeding, including intracranial hemorrhage (ICH, with prolonged prothrombin time, normal partial thromboplastin time and normal platelet counts, which are confirmed by the low level of FVII assay. Treatment consists of fresh frozen plasma (FFP, prothrombin complex concentrates (PCCs, and recombinant activated FVII to treat bleeding and prophylactic therapy. Here, we report four patients with FVII levels <5% (severe type who presented ICH during the neonatal period. The IVS6+1G>T was the most common (50% mutation identified in our study, followed by the K376X nonsense mutation (37.5%. In our study, we found that genetic information affected the severity of congenital FVII deficiency with ICH. Keywords: mutation analysis, factor VII deficiency, Thai children

  13. Quantitative PET Imaging of Tissue Factor Expression Using 18F-labled Active Site Inhibited Factor VII

    DEFF Research Database (Denmark)

    Nielsen, Carsten H; Erlandsson, Maria; Jeppesen, Troels E

    2016-01-01

    after the last imaging session, and tumor tissue was preserved for molecular analysis. A blocking experiment was performed in a second set of mice. The expression pattern of TF in the tumors was visualized by immunohistochemistry and the amount of TF in tumor homogenates was measured by ELISA......Tissue factor (TF) is up regulated in many solid tumors and its expression is linked to tumor angiogenesis, invasion, metastasis and prognosis. A non-invasive assessment of tumor TF expression status is therefore of obvious clinical relevance. Factor VII (FVII) is the natural ligand to TF. Here we...

  14. The F7 gene and clotting factor VII levels: dissection of a human quantitative trait locus.

    Science.gov (United States)

    Soria, Jose Manuel; Almasy, Laura; Souto, Juan Carlos; Sabater-Lleal, Maria; Fontcuberta, Jordi; Blangero, John

    2005-10-01

    Localization of human quantitative trait loci (QTLs) is now routine. However, identifying their functional DNA variants is still a formidable challenge. We present a complete dissection of a human QTL using novel statistical techniques to infer the most likely functional polymorphisms of a QTL that influence plasma levels of clotting factor VII (FVII), a risk factor for cardiovascular disease. Resequencing of 15 kb in and around the F7 gene identified 49 polymorphisms, which were then genotyped in 398 people. Using a Bayesian quantitative trait nucleotide (BQTN) method, we identified four to seven functional variants that completely account for this QTL. These variants include both rare coding variants and more common, potentially regulatory polymorphisms in intronic and promoter regions.

  15. Failure of Recombinant Activated Factor VII in Treatment of Diffuse Alveolar Hemorrhage due to Cryoglobulinemic Vasculitis

    Directory of Open Access Journals (Sweden)

    Dania Khoulani

    2014-01-01

    Full Text Available Diffuse alveolar hemorrhage (DAH is a serious complication of the small vessel vasculitis syndromes and carries a high mortality. Recombinant activated factor VII (rFVIIa is used to treat bleeding in patients with hemophilia and antibodies to factor VIII or IX. It is increasingly being used in life-threatening hemorrhage in a variety of other settings in which conventional therapy is unsuccessful. Randomized controlled trials of rFVIIa in DAH are lacking. However, several case reports have described a complete or sustained control of DAH using rFVIIa after patients failed to respond to medical treatment. There are no case reports in the literature describing the use or the failure of rFVIIa in DAH associated with cryoglobulinemic vasculitis. We here report the failure of rFVIIa to control DAH in a patient with CD5+ B-cell non-Hodgkin’s lymphoma and cryoglobulinemic vasculitis.

  16. Elective surgery on factor VIII inhibitor patients using continuous infusion of recombinant activated factor VII: plasma factor VII activity of 10 IU/ml is associated with an increased incidence of bleeding.

    Science.gov (United States)

    Smith, M P; Ludlam, C A; Collins, P W; Hay, C R; Wilde, J T; Grigeri, A; Melsen, T; Savidge, G F

    2001-10-01

    We examined recombinant activated factor VII (rVIIa) administered by continuous infusion to eight patients with inhibitors to factor VIII, undergoing elective surgery. rVIIa was infused at a fixed rate of 16.5 microg/kg/h for a median of 13.5 days (range 1-26). There was effective haemostasis at this infusion rate in only one of two minor procedures and two of six major operations. Three patients experienced excessive bleeding despite plasma factor VII activity around 10 IU/ml. Serious bleeding occurred in two other patients caused by procedural errors unrelated to rVIIa and required re-operation. The median rVIIa clearance on day 1 was 57 ml/h/kg (range 18-100) and on day 3 was 100 ml/h/kg (range 61-200). Clearance on the final infusion day was not significantly different from day 3. The infusion did not induce pathological activation of the coagulation mechanism. The only thrombotic adverse events were two episodes of superficial thrombophlebitis of the infused vein in one subject. In conclusion, the 16.5 microg/kg/h infusion rate reliably achieves plasma factor VII activity levels of 10 IU/ml, but this level does not provide reliable haemostasis.

  17. Plasma concentrations of blood coagulation factor VII measured by immunochemical and amidolytic methods

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Gram, J; Jespersen, J

    2000-01-01

    plasma, in cold activated plasma and in FVII deficient plasma. There was a positive correlation (r=0.96) between FVII:Ag and FVII:Am with slightly but significantly higher values for FVII:Ag (FVII:Ag= 106 U/ml and FVII:Am=100 U/ml; p ...Ever since the coagulant activity of blood coagulation factor VII (FVII:C) was identified as a risk indicator of cardiac death, a large number of studies have measured FVII protein concentrations in plasma. FVII protein concentrations are either measured immunologically with an ELISA method (FVII......:Ag) or estimated with an amidolytic method (FVII:Am). We have investigated whether FVII:Am is a valuable alternative to FVII:Ag. FVII:Ag and FVII:Am were measured in 147 plasma samples from blood donors, patients on oral anticoagulant therapy, postmenopausal women on hormone replacement therapy, in postprandial...

  18. The link between high-fat meals and postprandial activation of blood coagulation factor VII possibly involves kallikrein

    DEFF Research Database (Denmark)

    Larsen, L F; Marckmann, P; Bladbjerg, Else-Marie

    2000-01-01

    Contrary to low-fat meals, high-fat meals are known to cause postprandial factor VII (FVII) activation, but the mechanism is unknown. To study the postprandial FVII activation in detail, 18 young men consumed in randomized order high-fat or low-fat test meals. Fasting and non-fasting blood sample...

  19. Evidence supporting the use of recombinant activated factor VII in congenital bleeding disorders

    Directory of Open Access Journals (Sweden)

    Pär I Johansson

    2010-06-01

    Full Text Available Pär I Johansson, Sisse R OstrowskiCapital Region Blood Bank, Section for Transfusion Medicine, Rigshospitalet, University of Copenhagen, Copenhagen, DenmarkBackground: Recombinant activated factor VII (rFVIIa, NovoSeven® was introduced in 1996 for the treatment of hemophilic patients with antibodies against coagulation factor VIII or IX.Objective: To review the evidence supporting the use of rFVIIa for the treatment of patients with congenital bleeding disorders.Patients and methods: English-language databases were searched in September 2009 for reports of randomized controlled trials (RCTs evaluating the ability of rFVIIa to restore hemostasis in patients with congenital bleeding disorders.Results: Eight RCTs involving 256 hemophilic patients with antibodies against coagulation factors, also known as inhibitors, were identified. The evidence supporting the use of rFVIIa in these patients was weak with regard to dose, clinical setting, mode of administration, efficacy, and adverse events, given the limited sample size of each RCT and the heterogeneity of the studies.Conclusion: The authors suggest that rFVIIa therapy in hemophilic patients with inhibitors should be based on the individual’s ability to generate thrombin and form a clot, and not on the patient’s weight alone. Therefore, assays for thrombin generation, such as whole-blood thromboelastography, have the potential to significantly improve the treatment of these patients.Keywords: hemophilia, inhibitors, coagulation factor VIII, coagulation factor IX, rFVIIa, NovoSeven, FEIBA, hemostasis, RCT

  20. A new automated method for continuous registration of factor VII activation in vitro. Activation is accelerated by the concentration of factor VII and the activity state of the protein

    DEFF Research Database (Denmark)

    Bladbjerg, Else-Marie; Jespersen, J; Gram, J

    1994-01-01

    When a plasma sample is exposed to tissue factor, single-chain factor VII (FVII) is gradually converted to the active two-chain form (FVIIa). In the present study, we have constructed a measurement system, which allows continuous registration of the activation of FVII to FVIIa in vitro....... In this system, FVII activation follows parabolic kinetic after an initial lag-phase. The slope of the linear phase is a measure of the protein concentration of factor VII (FVIItotal), while the length of the non-linear phase represents the velocity of FVII activation. The time required for complete activation...... of FVII is inversely related to both FVIItotal and the relative amount of FVIIa in the sample. In future studies, this new measurement system will make it possible to study the process of FVII activation in different samples, and to examine how varying concentrations of exogenous added components affect...

  1. The use of recombinant-activated factor VII in von Willebrand disease: a case series.

    Science.gov (United States)

    von Depka, Mario; Hassan, Murtada; Blatnŷ, Jan; Smejkal, Petr; Vdovin, Vladimir

    2006-06-01

    Spontaneous and surgery-associated bleeding in patients with von Willebrand disease (vWD) cannot always be controlled with desmopressin or replacement therapy. This paper presents results on the use of recombinant-activated factor VII (rFVIIa) in patients with vWD included in the internet registry Haemostasis.com. Twenty-eight reports on the use of rFVIIa in vWD were identified from the database and included in this analysis. The bleeding episodes were classified as mild (n = 7), moderate (n = 16), or severe (n = 2), and were unspecified in three cases. The median dose of rFVIIa administered was 94 microg/kg body weight (40-127.3 microg/kg). Bleeding stopped in 23 of 27 evaluable patients (85%) and markedly decreased in three patients; the total response rate was 96% (26/27 patients). Response did not correlate with the type of vWD, the site or severity of the initial bleed, or the rFVIIa dose. Other replacement therapies were infrequently used, and their use was similar in the 24 h before and after rFVIIa administration. Eighteen patients also received antifibrinolytic treatment, but its impact on response was not recorded. Only one adverse event (mild fever) was observed. These cases suggest a role for rFVIIa as a safe and effective therapy for vWD.

  2. Non-fasting factor VII coagulant activity (FVII:C) increased by high-fat diet

    DEFF Research Database (Denmark)

    Bladbjerg, Else-Marie; Marckmann, P; Sandström, B

    1994-01-01

    Preliminary observations have suggested that non-fasting factor VII coagulant activity (FVII:C) may be related to the dietary fat content. To confirm this, we performed a randomised cross-over study. Seventeen young volunteers were served 2 controlled isoenergetic diets differing in fat content (20......% or 50% of energy). The 2 diets were served on 2 consecutive days. Blood samples were collected at 8.00 h, 16.30 h and 19.30 h, and analysed for triglycerides, FVII coagulant activity using human (FVII:C) or bovine thromboplastin (FVII:Bt), and FVII amidolytic activity (FVII:Am). The ratio FVII......:Bt/FVII:Am (a measure of FVII activation) increased from fasting levels on both diets, but most markedly on the high-fat diet. In contrast, FVII:Am (a measure of FVII protein) tended to decrease from fasting levels on both diets. FVII:C rose from fasting levels on the high-fat diet, but not on the low-fat diet...

  3. Coagulation factor VII R353Q polymorphism and the risk of puerperal cerebral venous thrombosis.

    Science.gov (United States)

    Kruthika-Vinod, T P; Nagaraja, Dindagur; Christopher, Rita

    2012-01-01

    Puerperal cerebral venous thrombosis (CVT) is a relatively common form of stroke in young women in India. The blood coagulation factor VII (FVII) R353Q polymorphism increases the risk for venous thrombosis. Our aim was to investigate the association of FVII R353Q polymorphism with the risk of puerperal CVT. A total of 100 women with puerperal CVT and 102 age-matched women without postpartum complications were investigated. FVII R353Q genotypes were identified using restriction fragment length polymorphism analysis. Our results showed that the homozygous FVII 353QQ genotype was present in 9% and 8% of patients and controls, respectively; and 42% of patients and 31.4% of controls had the heterozygous 353RQ genotype (odds ratio = 1.55, 95% confidence interval = 0.89-2.70; p = 0.243). Our findings suggest that the FVII R353Q polymorphism is not associated with increased risk for CVT occurring during the puerperal period in Indian women.

  4. Combined congenital dysfibrinogenemia and factor VII deficiency from mutations in the FGB and F7 genes.

    Science.gov (United States)

    Woo, Hye In; Park, In-Ae; Lee, Ki-O; Kim, Sun-Hee; Kim, Hee-Jin

    2012-07-01

    Dysfibrinogenemia and factor VII (FVII) deficiency are rare congenital coagulopathies. In this report, the authors describe a man with both defects confirmed by molecular genetic tests. The patient was a 51-year-old man referred for prolonged prothrombin time (PT) that had been accidentally detected on preoperative screening. He had no history of bleeding tendency even on occasions of surgery. Routine coagulation studies revealed prolonged PT (1.53 INR) and thrombin time (42.2 s), and decreased fibrinogen level (57 mg/dl) and FVII activity (44%). Direct sequencing analyses were performed on FGA, FGB, and FGG genes to confirm dysfibrinogenemia and on the F7 gene to confirm FVII deficiency. As a result, the patient was shown to be heterozygous for a point mutation in exon 8 of the FGB gene (c.1475A > G, p.*492Trpext*12; Fibrinogen Magdeburg II) and for a missense mutation in exon 6 of the F7 gene (c.466G  > A, p.Gly156Ser). To our knowledge, this is the first report on a case of combined dysfibrinogenemia and FVII deficiency confirmed by molecular genetic tests.

  5. Prevalence of factor VII deficiency and molecular characterization of the F7 gene in Brazilian patients.

    Science.gov (United States)

    Rodrigues, Dalva N; Siqueira, Lucia H; Galizoni, Andréa M; Arruda, Valder R; Annichino-Bizzacchi, Joyce M

    2003-04-01

    The prevalence of factor VII (FVII) deficiency in 267 Brazilian patients was estimated to be 4.1%, including one patient with significant bleeding, five with minor bleeding and five patients asymptomatic. Only one novel mutation 8926G F7 polymorphisms and FVII activity were found in these patients, as those with higher levels of FVII activity presented the genotype described in the literature as related to reduced FVII activity. As the R304Q mutation was the most frequent in these patients, and may be associated with an asymptomatic form of the disease, particularly in Blacks, we examined this mutation and FVII activity in 49 Blacks and 49 Caucasian blood donors with no clinical bleeding. None of the individuals showed the R304Q mutation, and FVII activity was normal in all of them, thus indicating that FVII deficiency is not common in normal individuals of these two ethnic groups in Brazil. This is the first study in South America to examine the prevalence and molecular basis of FVII deficiency, including the description of a novel mutation.

  6. First military use of activated Factor VII in an APC-III pelvic fracture.

    Science.gov (United States)

    Williams, D J; Thomas, G O R; Pambakian, S; Parker, P J

    2005-03-01

    A male soldier in shock with an APC-III pelvic fracture was flown to an Air Assault Surgical Group (AASG) of 16 Close Support Medical Regiment at Al Amarah in Iraq. A pelvic external fixator was applied and his condition stabilized. Ultrasound scanning (FAST) showed an absent bladder, and a spreading retroperitoneal haematoma combined with intra-abdominal, free blood. Unfortunately he continued to bleed and required transfusion with the unit's entire stock of type-specific blood. At emergency laparotomy, uncontrollable pelvic bleeding was encountered and the abdominal aorta required clamping above the iliac bifurcation. Branches of the right internal iliac artery were the source and this was ligated. Some bleeding continued post-operatively: administration of activated Factor VII was associated with a marked reduction in the oozing from his fixator pin-sites and an improvement in his pH from 7.1 to 7.3. In total, 25 units of blood were transfused, 8 of which were fresh whole blood donated by individual members of the AASG. The patient survived, returned to the UK, had his injuries reconstructed, and is currently undergoing rehabilitation. This case illustrates the benefits of forward resuscitation surgery in wartime and the need for a multidisciplinary approach to trauma care.

  7. Recombinant-activated factor VII in patients with uncontrolled bleeding: A retrospective observational analysis

    Directory of Open Access Journals (Sweden)

    Said D Abuhasna

    2012-01-01

    Full Text Available Background: Factor VIIa (recombinant has an off-label use to control life-threatening bleeding that is refractory to other measures and was shown to decrease transfusion requirements. Objective: The primary objective of this study was to assess the safety and effectiveness of factor VIIa (recombinant on blood transfusion requirements and coagulation parameters when used in patients whose bleeding was uncorrected by other means. The pharmacoeconomic impact for any discrepancy from our protocol was evaluated. Secondary outcomes included 4-hour and 28-day mortality, as well as safety of this agent in terms of thromboembolic complications. Materials and Methods: We retrospectively evaluated patients who received recombinant-activated factor VII (rFVIIa for uncontrolled bleeding from June 2008 to April 2011. The medical records of 33 patients were evaluated. Coagulation parameters and blood products were determined 24 hours before and 24 hours after administration of rFVIIa, and the results compared. Patients were also screened for any thromboembolic complications. Results: Administration of rFVIIa reduced blood transfusion requirements and improved coagulation parameters significantly (P<0.05. No thromboembolic complications were reported. Most of the dosing was consistent with those recommended in our institutional protocol, with discrepancies resulting in an average cost of $56 058. Moreover, pH was reported in only 67% of patients. All patients treated with rFVIIa survived up to 4 hours after receiving this agent, while the 28-day mortality was 24% (8/33. Conclusion: The use of rFVIIa appears to be safe and effective in promoting hemostasis, as evident from reducing transfusion requirements and improving the coagulation variables.

  8. Management of Pregnancy in a Chilean Patient with Congenital Deficiency of Factor VII and Glanzmann’s Thrombasthenia Variant

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    Nigel P. Murray

    2014-01-01

    Full Text Available Patients with inherited bleeding disorders are rare in obstetric practice but present with prolonged bleeding even after minor invasive procedures. They require a combined approach with obstetric and hematological management of each case, including the neonatal management of a possibly affected fetus. We present the case of a pregnancy in a patient with combined Factor VII deficiency and Glanzmann’s thrombasthenia, the successful obstetric and hematological management of the case, and a review of the literature.

  9. Mutagenesis in sequence encoding of human factor VII for gene therapy of hemophilia

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    B Kazemi

    2009-12-01

    Full Text Available "nBackground: Current treatment of hemophilia which is one of the most common bleeding disorders, involves replacement therapy using concentrates of FVIII and FIX .However, these concentrates have been associated with viral infections and thromboembolic complications and development of antibodies. "nThe use of recombinant human factor VII (rhFVII is effective  for the treatment of patients with  hemophilia A or B, who develop antibodies ( referred as inhibitors against  replacement therapy , because it induces coagulation independent of FVIII and FIX. However, its short half-life and high cost have limited its use. One potential solution to this problem may be the use of FVIIa gene transfer, which would attain continuing therapeutic levels of expression from a single injection. The aim of this study was to engineer a novel hFVII (human FVII gene containing a cleavage site for the intracellular protease and furin, by PCR mutagenesis "nMethods: The sequence encoding light and heavy chains of hFVII, were amplified by using hFVII/pTZ57R and specific primers, separately. The PCR products were cloned in pTZ57R vector. "nResults and discussion: Cloning was confirmed by restriction analysis or PCR amplification using specific primers and plasmid universal primers. Mutagenesis of sequence encoding light and heavy chain was confirmed by restriction enzyme. "nConclusion: In the present study, it was provided recombinant plasmids based on mutant form of DNA encoding light and heavy chains.  Joining mutant form of DNA encoding light chain with mutant heavy chain led to a new variant of hFVII. This variant can be activated by furin and an increase in the proportion of activated form of FVII. This mutant form of hFVII may be used for gene therapy of hemophilia.

  10. The link between high-fat meals and postprandial activation of blood coagulation factor VII possibly involves kallikrein

    DEFF Research Database (Denmark)

    Larsen, L F; Marckmann, P; Bladbjerg, Else-Marie

    2000-01-01

    Contrary to low-fat meals, high-fat meals are known to cause postprandial factor VII (FVII) activation, but the mechanism is unknown. To study the postprandial FVII activation in detail, 18 young men consumed in randomized order high-fat or low-fat test meals. Fasting and non-fasting blood samples...... that triglyceride-rich lipoproteins may activate prokallikrein. Neither plasma triglycerides nor kallikrein and activated FVII were statistically associated. This may suggest that additional factors are involved in the postprandial FVII activation. No clear evidence for a role of tissue factor expression...

  11. Double heterozygous mutations Gln100Leu and His348Gln of the F7 gene in a patient with factor VII deficiency.

    Science.gov (United States)

    Li, Min; Zheng, Fangxiu; Jin, Yanhui; Wang, Mingshan; Zhu, Liqing; Yang, Lihong

    2013-03-01

    A 25-year-old Chinese woman who had a history of easy bruising was admitted to hospital due to uncontrolled epistaxis. She showed factor VII activity level of 2% and factor VII antigen level of 4% of the normal value. We detected a novel missense mutation g.8355 A>T (p.Gln100Leu) in the second epidermal growth factor-like (EGF) domain and a g.11482 T>G (p.His348Gln) in the catalytic domain. Although the Gln100 residue is close to the junction of EGF-2 domain with the serine protease domain, we infer that the substitution of polar negatively charged Gln residue at the position 100 with introduction of nonpolar Leu residue may be likely to perturb proper folding, resulting in decreasing factor VII activity.

  12. R353Q polymorphism in the factor VII gene and cardiovascular risk in Heterozygous Familial Hypercholesterolemia: a case-control study

    Directory of Open Access Journals (Sweden)

    Pérez-Jiménez Francisco

    2011-04-01

    Full Text Available Abstract Background Heterozygous Familial Hypercholesterolemia (FH is a genetic disorder characterized by a high risk of cardiovascular disease. Certain polymorphisms of the factor VII gene have been associated with the development of coronary artery disease and there is a known association between factor VII levels and polymorphic variants in this gene. To date, no study has evaluated the association between factor VII and coronary artery disease in patients with FH. Results This case-control study comprised 720 patients (546 with FH and 174 controls. We determined the prevalence and allele frequencies of the R353Q polymorphism of factor VII, the plasma levels of factor VII antigen (FVII Ag and whether they could be predictive factors for cardiovascular risk. 75% (410 of the patients with FH were RR, 23% (127 RQ and 1.6% (9 QQ; in the control group 75.3% (131 were RR, 21.3% (37 RQ and 3.4% (6 QQ (p = 0.32. No statistically significant associations were observed in the distribution of genotypes and allele frequencies between case (FH and control groups. Nor did we find differences when we evaluated the relationship between the R353Q polymorphism and cardiovascular risk (including coronary disease, ischemic stroke and peripheral arterial disease, either in the univariate analysis or after adjustment for sex, age, arterial hypertension, body mass index, xanthomas, diabetes, smoking, HDLc and LDLc and lipid-lowering treatment. The FVII Ag concentrations behaved in a similar fashion, with no differences for the interaction between controls and those with FH (RR vs. RQ/QQ; p = 0.96. In the subgroup of patients with FH no association was found among cardiovascular disease, genotype and FVII Ag levels (RR vs. RQ/QQ; p = 0.97. Conclusions Our study did not find a direct relationship between cardiovascular risk in patients with Heterozygous Familial Hypercholesterolemia, the R353Q polymorphism of factor VII and FVII Ag levels.

  13. Group VII Ethylene Response Factors Coordinate Oxygen and Nitric Oxide Signal Transduction and Stress Responses in Plants1

    Science.gov (United States)

    Gibbs, Daniel J.; Conde, Jorge Vicente; Berckhan, Sophie; Prasad, Geeta; Mendiondo, Guillermina M.; Holdsworth, Michael J.

    2015-01-01

    The group VII ethylene response factors (ERFVIIs) are plant-specific transcription factors that have emerged as important regulators of abiotic and biotic stress responses, in particular, low-oxygen stress. A defining feature of ERFVIIs is their conserved N-terminal domain, which renders them oxygen- and nitric oxide (NO)-dependent substrates of the N-end rule pathway of targeted proteolysis. In the presence of these gases, ERFVIIs are destabilized, whereas an absence of either permits their accumulation; ERFVIIs therefore coordinate plant homeostatic responses to oxygen availability and control a wide range of NO-mediated processes. ERFVIIs have a variety of context-specific protein and gene interaction partners, and also modulate gibberellin and abscisic acid signaling to regulate diverse developmental processes and stress responses. This update discusses recent advances in our understanding of ERFVII regulation and function, highlighting their role as central regulators of gaseous signal transduction at the interface of ethylene, oxygen, and NO signaling. PMID:25944828

  14. Are olive oil diets antithrombotic? Diets enriched with olive, rapeseed, or sunflower oil affect postprandial factor VII differently

    DEFF Research Database (Denmark)

    Larsen, L.F.; Jespersen, J; Marckmann, P.

    1999-01-01

    BACKGROUND: The incidence of ischemic heart disease (IHD) in Crete was lower than expected on the basis of blood lipid concentrations of participants in the Seven Countries Study. A favorable effect of a high intake of olive oil on thrombogenesis may have contributed to this finding. OBJECTIVE: We...... compared the effects of virgin olive oil with those of rapeseed and sunflower oils on blood coagulation factor VII (FVII), a key factor in thrombogenesis. DESIGN: In a randomized and strictly controlled crossover study, 18 healthy young men consumed diets enriched with 5 g/MJ (19% of total energy) olive...... FVII (FVIIa) were 11.3 +/- 5.1 U/L lower after olive oil than after sunflower oil, an 18% reduction (P Olive oil also tended to cause lower FVIIa peak concentrations than did rapeseed oil (mean difference: 8.6 U/L, a 15% reduction; P = 0.09). There were no significant differences between diets...

  15. Recombinant factor VII (NovoSeven in intraoperative blood saving during neurosurgical treatment of the brain arteriovenous malformation

    Directory of Open Access Journals (Sweden)

    Novak Vesna

    2007-01-01

    Full Text Available Background. Cerebral arteriovenous (AV malformation causes, due to the increased blood flow through a malformation, a massive intraoperative bleeding complicating, so, surgical treatment. The use of intraoperative blood saving apparatus during surgery and a recombinant factor VII-a (NovoSeven significantly reduce complications during surgical treatment. Case report. We reported a case of surgical treatment of the patient with AV malformation of IV stage according to the Spetzler-Martin scale, in the brain. Due to a possible heavy bleeding we used a apparatus for intrasurgical blood recovery, Cell Saver, Sequestra 1 000, Medtronic, U.S.A., and recombinant human factor VIIa (rFVIIa - NovoSeven, NovoNordisk, Denmark to control bleeding and restore an adequate hemostasis. Conclusion. The use of an apparatus for intraoperative blood saving, as well as the NovoSeven preparation in the management of AV malformation of IV stage, showed to be successful.

  16. Evaluation of Aryoseven Safety (Recombinant Activated Factor VII) in Patients with Bleeding Disorders (An Observational Post-Marketing Surveillance Study)

    Science.gov (United States)

    Toogeh, Gholamreza; Abolghasemi, Hassan; Eshghi, Peyman; Managhchi, Mohammadreza; Shaverdi-niasari, Mohammadreza; Karimi, Katayoon; Roostaei, Samin; Emran, Neda; Abdollahi, Alireza

    2016-01-01

    Background: Recombinant activated factor VII induces hemostasis in patients with coagulopathy disorders. AryoSeven™ as a safe Iranian Recombinant activated factor VII has been available on our market. This study was performed to establish the safety of AryoSeven on patients with coagulopathy disorder. Methods: This single-center, descriptive, cross sectional study was carried out in Thrombus and Homeostasis Research Center ValiAsr Hospital during 2013-2014. Fifty one patients with bleeding disorders who received at least one dose of Aryoseven were enrolled. Patients’ demographic data and adverse effect of drug and reaction related to Aryoseven or previous usage of Recombinant activated FVII were recorded in questionnaires. Finally data were analyzed to compare side effects of Aryoseven and other Recombinant activated FVII brands. Results: Aryoseven was prescribed for 51 Patients. Of all participants with mean age 57.18+21.38 yr, 31 cases were male and 26 subjects had past history of recombinant activated FVII usage. Glanzman was the most frequent disorder followed by congenital FVII deficiency, hemophilia with inhibitors, factor 5 deficiency, acquired hemophilia, hemophilia A with inhibitor, and hemophilia A or B with inhibitor. The majority of bleeding episodes had occurred in joints. Three patients (5.9%) complained about adverse effects of Aryoseven vs. 11.5 % about adverse effects of other brands. However this difference was not significant, statistically. Conclusion: Based on monitor patients closely for any adverse events, we concluded that Aryoseven administration under careful weighing of benefit versus potential harm may comparable with other counterpart drugs. PMID:27799968

  17. LA SOMATOTROPINA BOVINA RECOMBINANTE Y LA REPRODUCCIÓN EN BOVINOS, OVINOS Y CAPRINOS

    OpenAIRE

    Joel Hernández-Cerón; C. Guillermo Gutierrez-Aguilar

    2013-01-01

    En vacas, la somatotropina bovina recombinante (rbST) se utiliza para incrementar la producción de leche. La rbST aumenta las concentraciones séricas del factor de crecimiento parecido a la insulina tipo I (IGF-I) y ambas hormonas regulan los procesos fisiológicos para incrementar la lactopoyesis. Además de sus efectos en la lactación, la somatotropina y el IGF-I favorecen la maduración del ovocito, tasa de fertilización, desarrollo embrionario temprano, función del cuerpo lúteo y reconocimie...

  18. Estrategias de obtención de proteínas recombinantes en Escherichia coli

    OpenAIRE

    José García; Zeila Santana; Lourdes Zumalacárregui; Marisel Quintana; Diamilé González; Gustavo Furrazola; Oscar Cruz

    2013-01-01

    La expresión de proteínas recombinantes se ha favorecido con el uso de Escherichia coli debido a su relativo bajo costo, alta densidad de cultivo, su fácil manipulación genética y a las diversas herramientas biotecnológicas disponibles que son compatibles. En este artículo se presentan algunas estrategias para la expresión de Escherichia coli; se destacan factores genéticos y fisiológicos que incluyen: número de copias del vector de expresión, características del gen, estabilidad del ácido ri...

  19. Estrategias de obtención de proteínas recombinantes en Escherichia coli

    OpenAIRE

    José García; Zeila Santana; Lourdes Zumalacárregui; Marisel Quintana; Diamilé González; Gustavo Furrazola; Oscar Cruz

    2013-01-01

    La expresión de proteínas recombinantes se ha favorecido con el uso de Escherichia coli debido a su relativo bajo costo, alta densidad de cultivo, su fácil manipulación genética y a las diversas herramientas biotecnológicas disponibles que son compatibles. En este artículo se presentan algunas estrategias para la expresión de Escherichia coli; se destacan factores genéticos y fisiológicos que incluyen: número de copias del vector de expresión, características del gen, estabilidad del ácido ri...

  20. The protein concentration of blood coagulation factor VII can be measured equally well in plasma and serum

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Overgaard, K; Gram, J

    1995-01-01

    In the Northwick Park Heart Study, the coagulant activity of factor VII (FVII:C) has been identified as a risk marker of ischaemic heart disease. In the fasting state, the protein concentration of FVII (FVII:Ag) might be an even better risk marker, because of the low coefficient of variation...... samples. Results were compared by means of linear regression, where y = 0.984 x +0.770, r = 0.96. No systematic variation existed between FVII:Ag in plasma and serum. The mean difference in FVII:Ag between plasma and serum was -1.17 (SD 11.92) arbitrary units, compared with a mean difference of 0.18 (SD 8.......31) arbitrary units between duplicate measurements of the same plasma dilution. Our findings indicate that there is a good agreement between FVII:Ag in plasma and serum. Udgivelsesdato: 1995-May...

  1. Successful use of rFVIIa for major breast surgery prophylaxis in congenital factor VII deficiency.

    Science.gov (United States)

    Varricchio, Antonio; Schettino, Michela; Ciampa, Antonio; Iannace, Carlo; Lo Conte, Domenico; Donnarumma, Bernadette; Vigorito, Raffaella; Lepore, Mariolina

    2014-06-24

    emorragiche. In questo articolo descriviamo un caso di una paziente di 46 anni con deficit congenito del fattore VII che si è sottoposta a trattamento chirurgico con successo grazie al trattamento con Novoseven prima della procedura. È stato utilizzato il modello riportato sotto per valutare i livelli di PTT e aPTT nel paziente. Per il prelievo ematico il sangue venoso della paziente è stato raccolto in provette di plastica (0.129 M di citrato di sodio, con sistema Vacutainer). Il tempo di protrombina è stato misurato con Recombiplastin (IL, Milano Italy), l’aPTT è stato misurato con l’APTT-SP (IL, Milano Italy). Come primo approccio profilattico alla paziente è stata utilizzata la vitamina K intramuscolo ma con scarsi risultati, poi si è valutato l’uso del Prothromplex, senza però ottenere un effetto significativo. L’ultimo step è stata la somministrazione di una terapia con Novoseven ® 15μg/kg somministrato 30 minuti prima dell’intervento and 4-5 h dopo la prima infusione. L’infusione del farmaco ha risolto i problemi di coagulazione permettendo il trattamento chirurgico, senza rischio nè emorragico nè trombotico per la paziente.

  2. Thrombin generation by activated factor VII on platelet activated by different agonists. Extending the cell-based model of hemostasis

    Directory of Open Access Journals (Sweden)

    Herrera Maria

    2006-04-01

    Full Text Available Abstract Background Platelet activation is crucial in normal hemostasis. Using a clotting system free of external tissue factor, we investigated whether activated Factor VII in combination with platelet agonists increased thrombin generation (TG in vitro. Methods and results TG was quantified by time parameters: lag time (LT and time to peak (TTP, and by amount of TG: peak of TG (PTG and area under thrombin formation curve after 35 minutes (AUC→35min in plasma from 29 healthy volunteers using the calibrated automated thrombography (CAT technique. TG parameters were measured at basal conditions and after platelet stimulation by sodium arachidonate (AA, ADP, and collagen (Col. In addition, the effects of recombinant activated FVII (rFVIIa alone or combined with the other platelet agonists on TG parameters were investigated. We found that LT and TTP were significantly decreased (p 35min were significantly increased (p 35min (but not PTG when compared to platelet rich plasma activated with agonists in the absence of rFVIIa. Conclusion Platelets activated by AA, ADP, Col or rFVIIa triggered TG. This effect was increased by combining rFVIIa with other agonists. Our intrinsic coagulation system produced a burst in TG independent of external tissue factor activity an apparent hemostatic effect with little thrombotic capacity. Thus we suggest a modification in the cell-based model of hemostasis.

  3. Group VII Ethylene Response Factors Coordinate Oxygen and Nitric Oxide Signal Transduction and Stress Responses in Plants.

    Science.gov (United States)

    Gibbs, Daniel J; Conde, Jorge Vicente; Berckhan, Sophie; Prasad, Geeta; Mendiondo, Guillermina M; Holdsworth, Michael J

    2015-09-01

    The group VII ethylene response factors (ERFVIIs) are plant-specific transcription factors that have emerged as important regulators of abiotic and biotic stress responses, in particular, low-oxygen stress. A defining feature of ERFVIIs is their conserved N-terminal domain, which renders them oxygen- and nitric oxide (NO)-dependent substrates of the N-end rule pathway of targeted proteolysis. In the presence of these gases, ERFVIIs are destabilized, whereas an absence of either permits their accumulation; ERFVIIs therefore coordinate plant homeostatic responses to oxygen availability and control a wide range of NO-mediated processes. ERFVIIs have a variety of context-specific protein and gene interaction partners, and also modulate gibberellin and abscisic acid signaling to regulate diverse developmental processes and stress responses. This update discusses recent advances in our understanding of ERFVII regulation and function, highlighting their role as central regulators of gaseous signal transduction at the interface of ethylene, oxygen, and NO signaling. © 2015 American Society of Plant Biologists. All Rights Reserved.

  4. Production of coagulation factor VII in human cell lines Sk-Hep-1 and HKB-11.

    Science.gov (United States)

    Corrêa de Freitas, Marcela Cristina; Bomfim, Aline de Sousa; Mizukami, Amanda; Picanço-Castro, Virgínia; Swiech, Kamilla; Covas, Dimas Tadeu

    2017-09-01

    Recombinant factor VII (rFVII) is the main therapeutic choice for hemophilia patients who have developed inhibitory antibodies against conventional treatments (FVIII and FIX). Because of the post-translational modifications, rFVII needs to be produced in mammalian cell lines. In this study, for the first time, we have shown efficient rFVII production in HepG2, Sk-Hep-1, and HKB-11 cell lines. Experiments in static conditions for a period of 96 h showed that HepG2-FVII produced the highest amounts of rhFVII, with an average of 1843 ng/mL. Sk-hep-1-FVII cells reached a maximum protein production of 1432 ng/mL and HKB-11-FVII cells reached 1468 ng/mL. Sk-Hep-1-rFVII and HKB-11-rFVII were selected for the first step of scale-up. Over 10 days of spinner flask culture, HKB-11 and SK-Hep-1 cells showed a cumulative production of rFVII of 152 μg and 202.6 μg in 50 mL, respectively. Thus, these human cell lines can be used for an efficient production of recombinant FVII. With more investment in basic research, human cell lines can be optimized for the commercial production of different bio therapeutic proteins. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Decreased plasma levels of factor II + VII + X correlate with increased levels of soluble cytokine receptors in patients with malaria and meningococcal infections

    DEFF Research Database (Denmark)

    Bygbjerg, I C; Hansen, M B; Rønn, A M;

    1997-01-01

    The levels of coagulation factors II + VII + X and of blood platelets (thrombocytes) as well as of cytokines and soluble cytokine receptors were studied in the patients with malaria or meningococcal infections. The coagulation factors were decreased particularly in the meningococcal patients, while...... necrosis factor-I (sTNF-RI) in patients with malaria and meningococcal infections. Elevated sIL-2R and sTNF-RI levels and decreased coagulation factors reverted to normal within 3-5 days after initiation of therapy in P. falciparum patients followed consecutively. Estimation of coagulation factors may...

  6. In vitro effects of heparin and tissue factor pathway inhibitor on factor VII assays. possible implications for measurements in vivo after heparin therapy

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Larsen, L F; Ostergaard, P;

    2000-01-01

    The coagulant activity of blood coagulation factor VII (FVII:C) can be lowered by changes in lifestyle and by therapeutic intervention, e.g. heparin infusion. The question is, however, whether FVII:C determined ex vivo is a valid measure of the FVII activity in vivo. We measured plasma FVII......:C, activated FVII (FVIIa), FVII protein (FVII:Ag), tissue factor pathway inhibitor (TFPI), triglycerides, and free fatty acids (FFA) before and 15 min after infusion of a bolus of unfractionated heparin (50 IU/kg body weight) in 12 healthy subjects. Additionally, we conducted in vitro experiments...... to investigate the effect of unfractionated heparin and TFPI, which is released from the endothelium by heparin, on FVII:C, FVIIa, and FVII:Ag. Heparin infusion decreased triglycerides and increased FFA and TFPI. This was accompanied by significant reductions in FVIIa, FVII:C and FVII:Ag. In vitro, anti...

  7. Syndromes, Disorders and Maternal Risk Factors Associated With Neural Tube Defects (VII

    Directory of Open Access Journals (Sweden)

    Chih-Ping Chen

    2008-09-01

    Full Text Available Neural tube defects (NTDs may be associated with syndromes, disorders and maternal risk factors. This article provides a comprehensive review of the syndromes, disorders and maternal risk factors associated with NTDs, including DK phocomelia syndrome (von Voss-Cherstvoy syndrome, Siegel-Bartlet syndrome, fetal warfarin syndrome, craniotelencephalic dysplasia, Czeizel-Losonci syndrome, maternal cocaine abuse, Weissenbacher-Zweymüller syndrome, parietal foramina (cranium bifidum, Apert syndrome, craniomicromelic syndrome, XX-agonadism with multiple dysraphic lesions including omphalocele and NTDs, Fryns microphthalmia syndrome, Gershoni-Baruch syndrome, PHAVER syndrome, periconceptional vitamin B6 deficiency, and autosomal dominant Dandy-Walker malformation with occipital cephalocele. NTDs associated with these syndromes, disorders and maternal risk factors are a rare but important cause of NTDs. The recurrence risk and the preventive effect of maternal folic acid intake in NTDs associated with syndromes, disorders and maternal risk factors may be different from those of nonsyndromic multifactorial NTDs. Perinatal diagnosis of NTDs should alert doctors to the syndromes, disorders and maternal risk factors associated with NTDs, and prompt thorough etiologic investigation and genetic counseling.

  8. Successful Control of Massive Bleeding in a Child with Burkitt’s Lymphoma via a Biosimilar Recombinant Activated Factor VII (AryoSeven™

    Directory of Open Access Journals (Sweden)

    Kourosh Goudarzi Pour

    2016-01-01

    Full Text Available We describe a case of a 4-year-old girl with Burkitt’s lymphoma, who suffered from a massive gastrointestinal hemorrhage 3 days after chemotherapy. In spite of applying the common practice in correction of coagulopathy, thrombocytopenia persisted and bleeding became life-threatening. In the present case report, we report a successful control of bleeding with a single-dose administration of a biosimilar recombinant activated human factor VII (AryoSeven.

  9. Decreased plasma levels of activated factor VII in patients with deep vein thrombosis

    NARCIS (Netherlands)

    Schut, A. M.; Meijers, J. C. M.; Lisman-van Leeuwen, Y.; van Montfoort, M. L.; Roest, M.; de Groot, P. G.; Urbanus, R. T.; Coppens, M.; Lisman, T.

    2015-01-01

    BackgroundThe initiating trigger in the development of deep vein thrombosis (DVT) remains unidentified. It has been suggested that tissue factor (TF)-bearing microparticles play a key role, which indicates a role for the TF pathway in the initiation of DVT. ObjectiveTo assess the role of the TF path

  10. HEREDITARY FACTOR VII DEFICIENCY IN THE ASIAN ELEPHANT (ELEPHAS MAXIMUS) CAUSED BY A F7 MISSENSE MUTATION.

    Science.gov (United States)

    Lynch, Michael; McGrath, Ken; Raj, Karthik; McLaren, Philippa; Payne, Karen; McCoy, Richard; Giger, Urs

    2017-04-01

    Hereditary disorders and genetic predispositions to disease are rarely reported in captive and free-ranging wildlife, and none have been definitively identified and characterized in elephants. A wild-caught, 41-yr-old male Asian elephant ( Elephas maximus ) without an apparent increased bleeding tendency was consistently found to have prolonged prothrombin times (PTs, mean=55±35 s) compared to 17 other elephants (PT=10±2 s). This elephant's partial thromboplastin times (PTT) fell within the normal range of the other elephants (12-30 s). A prolonged PT in the presence of a normal PTT suggests disruption of the extrinsic pathway via deficiency of coagulation Factor VII (FVII). This elephant's plasma FVII activity was very low (2%) compared to that of 15 other elephants (57-80%), but other coagulation factors' activities did not differ from the control elephants. Sequencing of genomic DNA from ethylenediaminetetraacetic acid blood revealed a single homozygous point mutation (c.202A>G) in the F7 gene of the FVII deficient elephant that was not present in unrelated elephants. This mutation causes an amino acid substitution (p.Arg68Gly) that is predicted to be deleterious. Two living offspring of the affected elephant were heterozygous for the mutation and had normal plasma FVII activities and coagulation profiles. Tissue from a third offspring, a deceased calf, was utilized to show that it was also a heterozygote. A DNA test has been developed to enable the screening of additional elephants for this mutation. Consistent with FVII deficiency investigations in other species, the condition did not cause a serious bleeding tendency in this individual elephant.

  11. The unsialylated subpopulation of recombinant activated factor VII binds to the asialo-glycoprotein receptor (ASGPR) on primary rat hepatocytes.

    Science.gov (United States)

    Seested, Torben; Nielsen, Hanne M; Christensen, Erik I; Appa, Rupa S

    2010-12-01

    Recombinant activated factor VII (rFVIIa; NovoSeven®) is a heterogeneously glycosylated serine protease used for treatment of haemophiliacs with inhibitors. The drug substance contains a subpopulation consisting of ~20% of rFVIIa molecules which are unsialylated and consists of carbohydrate moieties with terminally exposed galactose and N-acetyl-D-galactosamine (GalNAc). Recently, data from an in situ perfused liver model showed that a subpopulation of rFVIIa, appearing to be unsialylated rFVIIa, was cleared by the liver, thus suggesting a carbohydrate-moiety mediated mechanism. The parenchymal cells of the liver, hepatocytes, are known to abundantly express functional carbohydrate-specific receptors and in this study we therefore used primary rat hepatocytes to study binding and intracellular fate of rFVIIa at a cellular level. Immunofluorescence microscopy showed that rFVIIa was distributed into distinct intracellular vesicles and electron microscopic autoradiography revealed that radioiodinated rFVIIa distributed only into cytoplasmic free vesicles resembling endosomes and lysosomes. These findings suggest that endocytosis of rFVIIa in hepatocytes could be partly mediated via initial membrane binding to a receptor. Quantitative binding studies showed that the presence of excess unlabelled asialo-orosomucoid, asialo-rFVIIa and GalNAc significantly decreased binding of 125I-rFVIIa. An antibody which specifically binds to the carbohydrate recognition domain of the asialoglycoprotein receptor (ASGPR) significantly decreased binding of asialo-rFVIIa by ~36% and rFVIIa by ~19%. Together our data showed that a receptor-mediated mechanism involving the ASGPR is able to bind a subpopulation of unsialylated rFVIIa, while a hepatic mechanism for binding and clearing sialylated rFVIIa is still unknown.

  12. Impaired secretion of carboxyl-terminal truncated factor VII due to an F7 nonsense mutation associated with FVII deficiency.

    Science.gov (United States)

    Tanaka, Ryoko; Nakashima, Daisuke; Suzuki, Atsuo; Miyawaki, Yuhri; Fujimori, Yuta; Yamada, Takayuki; Takagi, Akira; Murate, Takashi; Yamamoto, Koji; Katsumi, Akira; Matsushita, Tadashi; Naoe, Tomoki; Kojima, Tetsuhito

    2010-03-01

    Factor VII (FVII) is a vitamin K-dependent glycoprotein secreted into the blood circulation from hepatic cells. We investigated the molecular basis of the congenital FVII deficiency found in a Japanese patient. We analyzed the F7 gene of the patient, who was diagnosed with a FVII deficiency at pregnancy. We expressed a carboxyl-terminal truncated FVII (Arg462X FVII) corresponding to the identified mutation in CHO-K1 cells. To study roles of the carboxyl-terminus in the secretion of FVII, we also expressed a series of recombinant FVIIs deleted of limited numbers of carboxyl-terminal amino acids (462Arg-466Pro). We identified a nonsense mutation (c.1384C>T: p.Arg462X) in F7, leading to a lack of five amino acids in the carboxyl-terminus. In expression experiments, Arg462X FVII was undetectable not only by Western blotting, but also by ELISA. A Western blot analysis of the truncated FVIIs revealed that all mutants were expressed in the cells the same as the wild type, but were secreted into the culture medium in lesser amounts than the wild type depending on the length of the deletion, which was confirmed by ELISA. Arg462X FVII did not colocalize with the Golgi on immunofluorescence staining, suggesting that it might be retained in the ER and degraded in the cell. The carboxyl-terminal amino acids of FVII play an important role in its secretion, and the p.Arg462X mutation was likely to have caused the FVII deficiency in this patient. (c) 2009 Elsevier Ltd. All rights reserved.

  13. [Risk assessment of thrombotic events after the use of activated factor VII].

    Science.gov (United States)

    Martínez López, M C; Alcaraz Romero, A J; Martínez López, A B; Fernández-Llamazares, C M; Ramos Navarro, C

    2013-09-01

    The objective of this study was to analyze the incidence of thrombotic complications related to recombinant human factor viia (rFVIIa) therapy for severe postoperative bleeding in cardiac surgery. A retrospective matched case-control study was conducted over two years, including 72 children admitted to intensive care unit and treated with rFVIIa because of a severe bleeding during or after cardiac surgery. A control group of 63 patients was chosen, who were statistically comparable in sex, weight, diagnosis, surgical risk according RASCH-1 score, and surgical characteristics, was chosen. There were no significant differences between cases and controls either in the rate of thrombosis (20% vs 28%, P=.540), or in the mortality rate (16% vs 9.5%, P=.208). In our study, the rFVIIa therapy was shown to be useful in controlling severe operative bleeding in pediatric cardiac surgery, but does not seem to increase the risk of thrombotic complications or mortality rate in the postoperative period. Copyright © 2012 Asociación Española de Pediatría. Published by Elsevier Espana. All rights reserved.

  14. Abnormal secretion and function of recombinant human factor VII as the result of modification to a calcium binding site caused by a 15-base pair insertion in the F7 gene.

    Science.gov (United States)

    Peyvandi, F; Carew, J A; Perry, D J; Hunault, M; Khanduri, U; Perkins, S J; Mannucci, P M; Bauer, K A

    2001-02-15

    A case of a novel mutation in the F7 gene that results in factor VII coagulant activity (VII:c) of less than 1% and VII antigen (VII:Ag) levels of 10% is presented. DNA analysis revealed a homozygous 15-base pair (bp) in-frame insertion-type mutation at nucleotide 10554. This insertion consisted of a duplication of residues leucine (L)213 to aspartic acid (D)217 (leucine, serine, glutamic acid, histidine, and aspartic acid), probably arising by slipped mispairing between 2 copies of a direct repeat (GCGAGCACGAC) separated by 4 bp. Molecular graphic analyses showed that the insertion is located at the surface of the catalytic domain in an exposed loop stabilized by extensive salt-bridge and hydrogen bond formation at which the calcium binding site is located. The mutation probably interferes with protein folding during VII biosynthesis and/or diminishes functional activity through the loss of calcium binding. In vitro expression studies demonstrated that the levels of VII:Ag in lysates of cells transfected with wild type VII (VIIWT) were equivalent to those with mutant type VII (VIIMT), but the level of secreted VIIMT was 5% to 10% that of VIIWT. Pulse chase studies demonstrated that VIIMT did not accumulate intracellularly, and studies with inhibitors of protein degradation showed that recombinant VIIMT was partially degraded in the pre-Golgi compartment. Accordingly, only small amounts of VIIMT with undetectable procoagulant activity were secreted into conditioned media. These results demonstrate that a combination of secretion and functional defects is the mechanism whereby this insertion causes VII deficiency.

  15. Use of activated recombinant factor VII for severe coagulopathy post ventricular assist device or orthotopic heart transplant

    Directory of Open Access Journals (Sweden)

    Despotis George J

    2007-07-01

    Full Text Available Abstract Background Ventricular assist devices(VAD implantation/removal is a complex surgical procedure with perioperative bleeding complications occurring in nearly half of the cases. Recombinant activated factor VII (rFVIIa has been used off-label to control severe hemorrhage in surgery and trauma. We report here our experience with rFVIIa as a rescue therapy to achieve hemostasis in patients undergoing orthotopic heart transplant (OHT and/or VAD implantation. Methods A retrospective review was conducted from Jan 03 to Aug 05 for patients who received rFVIIa for the management of intractable bleeding unresponsive to standard hemostatic blood component therapy. Blood loss and the quantity of blood products, prior to, and for at least 12 hours after, administration of rFVIIa were recorded. Results Mean patient age was 53, (38–64 yrs, mean dose of rFVIIa administered was 78.3 μg/kg (24–189 μg/kg in 1–3 doses. All patients received the drug either intraoperatively or within 6 hours of arrival in ICU. Mean transfusion requirements and blood loss were significantly reduced after rFVIIa administration (PRBC's; 16.9 ± 13.3 to 7.1 ± 6.9 units, FFP; 13.1 ± 8.2 to 4.1 ± 4.9 units, platelets; 4.0 ± 2.8 to 2.1 ± 2.2 units, p Conclusion In this review, there was a significant decrease in transfusion requirement and blood loss after rFVIIa administration. Although, 5/17 developed thromboembolic complications, these patients may have been at higher risk based on the multiple modality therapy used to manage intractable bleeding. Nevertheless, the exact role of rFVIIa with respect to development of thromboembolic complications cannot be clearly determined. Further investigation is needed to determine rFVIIa's safety and its effectiveness in improving postoperative morbidity and mortality.

  16. An engineered tale-transcription factor rescues transcription of factor VII impaired by promoter mutations and enhances its endogenous expression in hepatocytes.

    Science.gov (United States)

    Barbon, Elena; Pignani, Silvia; Branchini, Alessio; Bernardi, Francesco; Pinotti, Mirko; Bovolenta, Matteo

    2016-06-24

    Tailored approaches to restore defective transcription responsible for severe diseases have been poorly explored. We tested transcription activator-like effectors fused to an activation domain (TALE-TFs) in a coagulation factor VII (FVII) deficiency model. In this model, the deficiency is caused by the -94C > G or -61T > G mutation, which abrogate the binding of Sp1 or HNF-4 transcription factors. Reporter assays in hepatoma HepG2 cells naturally expressing FVII identified a single TALE-TF (TF4) that, by targeting the region between mutations, specifically trans-activated both the variant (>100-fold) and wild-type (20-40-fold) F7 promoters. Importantly, in the genomic context of transfected HepG2 and transduced primary hepatocytes, TF4 increased F7 mRNA and protein levels (2- to 3-fold) without detectable off-target effects, even for the homologous F10 gene. The ectopic F7 expression in renal HEK293 cells was modestly affected by TF4 or by TALE-TF combinations. These results provide experimental evidence for TALE-TFs as gene-specific tools useful to counteract disease-causing promoter mutations.

  17. Safety and efficacy of recombinant activated factor VII: a randomized placebo-controlled trial in the setting of bleeding after cardiac surgery

    DEFF Research Database (Denmark)

    Gill, Ravi; Herbertson, Mike; Vuylsteke, Alain

    2009-01-01

    BACKGROUND: Blood loss is a common complication of cardiac surgery. Evidence suggests that recombinant activated factor VII (rFVIIa) can decrease intractable bleeding in patients after cardiac surgery. Our objective was to investigate the safety and possible benefits of rFVIIa in patients who bleed...... after cardiac surgery. METHODS AND RESULTS: In this phase II dose-escalation study, patients who had undergone cardiac surgery and were bleeding were randomized to receive placebo (n=68), 40 microg/kg rFVIIa (n=35), or 80 microg/kg rFVIIa (n=69). The primary end points were the number of patients...... for treating bleeding after cardiac surgery, but caution should be applied and further clinical trials are required because there is an increase in the number of critical serious adverse events, including stroke, in those patients randomized to receive rFVIIa....

  18. Protein replacement therapy and gene transfer in canine models of hemophilia A, hemophilia B, von willebrand disease, and factor VII deficiency.

    Science.gov (United States)

    Nichols, Timothy C; Dillow, Aaron M; Franck, Helen W G; Merricks, Elizabeth P; Raymer, Robin A; Bellinger, Dwight A; Arruda, Valder R; High, Katherine A

    2009-01-01

    Dogs with hemophilia A, hemophilia B, von Willebrand disease (VWD), and factor VII deficiency faithfully recapitulate the severe bleeding phenotype that occurs in humans with these disorders. The first rational approach to diagnosing these bleeding disorders became possible with the development of reliable assays in the 1940s through research that used these dogs. For the next 60 years, treatment consisted of replacement of the associated missing or dysfunctional protein, first with plasma-derived products and subsequently with recombinant products. Research has consistently shown that replacement products that are safe and efficacious in these dogs prove to be safe and efficacious in humans. But these highly effective products require repeated administration and are limited in supply and expensive; in addition, plasma-derived products have transmitted bloodborne pathogens. Recombinant proteins have all but eliminated inadvertent transmission of bloodborne pathogens, but the other limitations persist. Thus, gene therapy is an attractive alternative strategy in these monogenic disorders and has been actively pursued since the early 1990s. To date, several modalities of gene transfer in canine hemophilia have proven to be safe, produced easily detectable levels of transgene products in plasma that have persisted for years in association with reduced bleeding, and correctly predicted the vector dose required in a human hemophilia B liver-based trial. Very recently, however, researchers have identified an immune response to adeno-associated viral gene transfer vector capsid proteins in a human liver-based trial that was not present in preclinical testing in rodents, dogs, or nonhuman primates. This article provides a review of the strengths and limitations of canine hemophilia, VWD, and factor VII deficiency models and of their historical and current role in the development of improved therapy for humans with these inherited bleeding disorders.

  19. Using a minigene approach to characterize a novel splice site mutation in human F7 gene causing inherited factor VII deficiency in a Chinese pedigree.

    Science.gov (United States)

    Yu, T; Wang, X; Ding, Q; Fu, Q; Dai, J; Lu, Y; Xi, X; Wang, H

    2009-11-01

    Factor VII deficiency which transmitted as an autosomal recessive disorder is a rare haemorrhagic condition. The aim of this study was to identify the molecular genetic defect and determine its functional consequences in a Chinese pedigree with FVII deficiency. The proband was diagnosed as inherited coagulation FVII deficiency by reduced plasma levels of FVII activity (4.4%) and antigen (38.5%). All nine exons and their flanking sequence of F7 gene were amplified by polymerase chain reaction (PCR) for the proband and the PCR products were directly sequenced. The compound heterozygous mutations of F7 (NM_000131.3) c.572-1G>A and F7 (NM_000131.3) c.1165T>G; p.Cys389Gly were identified in the proband's F7 gene. To investigate the splicing patterns associated with F7 c.572-1G>A, ectopic transcripts in leucocytes of the proband were analyzed. F7 minigenes, spanning from intron 4 to intron 7 and carrying either an A or a G at position -1 of intron 5, were constructed and transiently transfected into human embryonic kidney (HEK) 293T cells, followed by RT-PCR analysis. The aberrant transcripts from the F7 c.572-1G>A mutant allele were not detected by ectopic transcription study. Sequencing of the RT-PCR products from the mutant transfectant demonstrated the production of an erroneously spliced mRNA with exon 6 skipping, whereas a normal splicing occurred in the wide type transfectant. The aberrant mRNA produced from the F7 c.572-1G>A mutant allele is responsible for the factor VII deficiency in this pedigree.

  20. Effect of N-acetylcysteine on the accuracy of the prothrombin time assay of plasma coagulation factor II plus VII plus X activity in subjects infused with the drug. Influence of time and temperature

    DEFF Research Database (Denmark)

    Thorsen, S.; Teisner, A.; Jensen, S.A.;

    2009-01-01

    Objectives: The prothrombin time (PT) assay of factor II+VII+X activity is an important predictor of liver damage in paracetamol poisoned patients. It complicates interpretation of results that the antidote, acetylcysteine (NAC) depresses this activity. The aim was to investigate if NAC influences...

  1. Effect of N-acetylcysteine on the accuracy of the prothrombin time assay of plasma coagulation factor II+VII+X activity in subjects infused with the drug. Influence of time and temperature

    DEFF Research Database (Denmark)

    Thorsen, Sixtus; Teisner, Ane; Jensen, Søren Astrup;

    2009-01-01

    OBJECTIVES: The prothrombin time (PT) assay of factor II+VII+X activity is an important predictor of liver damage in paracetamol poisoned patients. It complicates interpretation of results that the antidote, acetylcysteine (NAC) depresses this activity. The aim was to investigate if NAC influences...

  2. Tratamiento con eritropoyetina humana recombinante Human recombinant erythropoietin therapy

    Directory of Open Access Journals (Sweden)

    Hugo Donato

    2006-02-01

    Full Text Available La eritropoyetina recombinante (rHuEPO se ha transformado en la citoquina más utilizada terapéuticamente en el mundo. Luego del éxito obtenido en pacientes con insuficiencia renal terminal, se pudo establecer la utilidad de la terapia con rHuEPO para mejorar otras anemias, incluso en pacientes pediátricos y neonatos. El tratamiento o la prevención de la anemia del prematuro mediante el uso de rHuEPO llevó a una significativa reducción en cantidad de transfusiones y en exposición a dadores. Aún debe establecerse una clara definición sobre cuáles niños prematuros deben recibir tratamiento rutinariamente. Otras indicaciones en período neonatal incluyen anemias hiporregenerativas y hemolíticas. La eficacia de la rHuEPO en niños mayores, con excepción de la insuficiencia renal crónica, no ha sido tan exhaustivamente evaluada como en adultos. Mientras que durante los últimos años se han realizado gran cantidad de estudios en adultos con anemia asociada al cáncer o a infección por HIV, permitiendo establecer conclusiones claras sobre su eficacia, sólo escasa cantidad de estudios con pequeño número de pacientes han sido realizados en niños. Hasta la fecha, los resultados sugieren que la terapia con rHuEPO en niños es tan útil como en adultos, pero la realización de estudios aleatorizados prospectivos incluyendo gran número de pacientes es esencial para alcanzar conclusiones definitivas. Los resultados de estudios dirigidos a evaluar la eficacia de la rHuEpo para mantener una dosis adecuada de ribavirina en pacientes en tratamiento por hepatitis C son alentadores. La utilización potencial de los efectos no hemopoyéticos de la rHuEPO en neonatos es un terreno novedoso y apasionante. El rol de la Epo como citoprotector para sistema nervioso central y mucosa intestinal está bajo investigación exhaustiva.Recombinant human erythropoietin (rHuEpo has become the most widely used cytokine in the world. Following the success of

  3. Fokker D.VII

    Science.gov (United States)

    1923-01-01

    Fokker D.VII: The Fokker D.VII was one of the best fighter aircraft of World War I, and was the only weapon used by the Central Powers specifically mentioned in the Versailles Treaty. The Central Powers surrendered 142 at the close of the war, and the Fokker company sold even more to the U. S. Air Service. Several were flown at Langley Field, but this one was the sole example operated by the NACA.

  4. Estrategias de obtención de proteínas recombinantes en Escherichia coli

    Directory of Open Access Journals (Sweden)

    José García

    2013-08-01

    Full Text Available La expresión de proteínas recombinantes se ha favorecido con el uso de Escherichia coli debido a su relativo bajo costo, alta densidad de cultivo, su fácil manipulación genética y a las diversas herramientas biotecnológicas disponibles que son compatibles. En este artículo se presentan algunas estrategias para la expresión de Escherichia coli; se destacan factores genéticos y fisiológicos que incluyen: número de copias del vector de expresión, características del gen, estabilidad del ácido ribonucleico mensajero, promotor empleado, cepa utilizada, composición del medio de cultivo, parámetros de operación en el fermentador y también se abordan la conservación de cepas y la estrategia de cultivo y purificación.

  5. Estudio de inmunogenicidad para dos vacunas recombinantes contra hepatitis B

    Directory of Open Access Journals (Sweden)

    O. Juliao

    1991-12-01

    Full Text Available Este estudio compara la inmunogenicidad (seroconversión, seroprotección e Hiperrespuesta, producida por dos vacunas recombinantes contra la hepatitis B (Engerix-B de Bélgica y Cubana, en dos esquemas (012 y 016 meses, empleando los métodos de cuantificación para Anti-HBsAg (Abbott y Organón, los cuales fueron también comparados. En el estudio participaron 257 voluntarios,  divididos al azar en 4 grupos (dos vacunas, dos esquemas. Resultados: los dos métodos de Abbon y Organon, no presentan diferencias estadísticas significativas. La vacuna cubana muestra una mayor respuesta inmunogénica para dos dosis de vacuna y para el esquema 012. No hay diferencia entre los esquemas 012 y 016 y en el esquema 016 no se ven diferencias estadísticamente significativas con la vacuna Engerix-B. En esta Última el esquema 016 muestra mejores resultados que el 012.

  6. Genotype–phenotype relationship of F7 R353Q polymorphism and plasma factor VII coagulant activity in Asian Indian families predisposed to coronary artery disease

    Indian Academy of Sciences (India)

    Jayashree Shanker; Ganapathy Perumal; Arindam Maitra; Veena S. Rao; B. K. Natesha; Shibu John; Sridhar Hebbagodi; Vijay V. Kakkar

    2009-12-01

    Elevated factor VII (FVII) level is a risk factor for coronary artery disease (CAD). We investigated the role of R353Q polymorphism in the F7 gene in 139 Indian families with CAD, comprising of 222 affected subjects, 105 unaffected subjects and 126 affected sibling pairs. Plasma per cent FVIIc activity (FVII.c activity) differed significantly across R353Q genotype ($P \\lt 0.0001$). Frequency of subjects with RR and QQ genotypes were higher in 4th quartile and 1st quartile of FVII.c activity, respectively ($P \\lt 0.0001$). F7 R353Q SNP was able to explain up to 7% of variation in FVII.c activity by regression analysis and an additive genetic component of variance of 28.04% by heritability analysis. Quantitative trait loci analysis showed suggestive linkage evidence of F7 SNP with per cent FVII.c activity (LOD score $-1.82$; $P = 0.002$). Individuals with RR and RQ genotypes carried an OR of 2.071 (95% c.i. = 1.506–2.850) and 2.472 (95% c.i. = 1.679–3.641), respectively, towards CAD risk. There was significant correlation of FVII.c activity with lipid markers, particularly among those with RR and RQ genotype after covariate adjustment. In conclusion, the F7 R353Q SNP appears to moderately influence plasma FVII.c activity and risk of CAD in Indians.

  7. Genotype-phenotype relationship of F7 R353Q polymorphism and plasma factor VII coagulant activity in Asian Indian families predisposed to coronary artery disease.

    Science.gov (United States)

    Shanker, Jayashree; Perumal, Ganapathy; Maitra, Arindam; Rao, Veena S; Natesha, B K; John, Shibu; Hebbagodi, Sridhar; Kakkar, Vijay V

    2009-12-01

    Elevated factor VII (FVII) level is a risk factor for coronary artery disease (CAD). We investigated the role of R353Q polymorphism in the F7 gene in 139 Indian families with CAD, comprising of 222 affected subjects, 105 unaffected subjects and 126 affected sibling pairs. Plasma per cent FVIIc activity (FVII.c activity) differed ignificantly across R353Q genotype (P F7 R353Q SNP was able to explain up to 7% of variation in FVII.c activity by regression analysis and an additive genetic component of variance of 28.04% by heritability analysis. Quantitative trait loci analysis showed suggestive linkage evidence of F7 SNP with per cent FVII.c activity (LOD score -1.82; P = 0.002). Individuals with RR and RQ genotypes carried an OR of 2.071 (95% c.i. = 1.506-2.850) and 2.472 (95% c.i. = 1.679-3.641), espectively, towards CAD risk. There was significant correlation of FVII.c activity with lipid markers, particularly among those with RR and RQ genotype after covariate adjustment. In conclusion, the F7 R353Q SNP appears to moderately influence plasma FVII.c activity and risk of CAD in Indians.

  8. Lethal factor VII deficiency due to novel mutations in the F7 promoter: functional analysis reveals disruption of HNF4 binding site.

    Science.gov (United States)

    Giansily-Blaizot, Muriel; Lopez, Estelle; Viart, Victoria; Chafa, Ouerdia; Tapon-Bretaudière, Jacqueline; Claustres, Mireille; Taulan, Magali

    2012-08-01

    Hereditary factor VII (FVII) deficiency is a rare autosomal recessive disorder. Deleterious mutations that prevent the synthesis of any amount of functional FVII have been associated with life-threatening haemorrhage in neonates. Here we report two infants, of Maghrebian origin, who suffered a fatal spontaneous cerebral haemorrhage. Investigation of the molecular basis for their severe FVII deficiency revealed novel mutations in a homozygous state within the F7 gene promoter: a single nucleotide substitution (c.-65G>C) and a 2bp deletion (c.-60_-59delTT). To determine whether these promoter variants were responsible for the FVII deficiency, computer-assisted sequence analyses were performed. The data predicted a disrupted binding of both HNF4 and COUP-TF transcription factors with each variant. Concordantly, experimental results revealed an altered HNF4-induced transactivation in the promoter mutated variants. The execution of functional tests is critical to ensuring a complete understanding of the effect of any promoter mutant on FVII deficiency. Only then can an accurate molecular diagnosis be made and further genetic counselling and prenatal diagnosis be offered.

  9. Estudio en poblaciones seleccionadas de la fiabilidad de nuevos protocolos de detección de consumo de hormonas recombinantes (hgH y EPO)

    OpenAIRE

    Abellán Sánchez, María Rosario

    2006-01-01

    Las hormonas recombinantes eritropoyetina (EPO) y hormona de crecimiento (GH), prácticamente iguales a las endógenas y de corta vida media en circulación, son de difícil detección directa en el control antidopaje. Se determinaron los valores poblacionales de los biomarcadores indirectos EPO, receptor soluble de la transferrina, insulin-like growth factor-I (IGF-I) y procolágeno tipo III péptido (P-III-P), en poblaciones seleccionadas de deportistas, y el efecto del ejercicio y los distintos t...

  10. F7 gene variants modulate protein levels in a large cohort of patients with factor VII deficiency. Results from a genotype-phenotype study.

    Science.gov (United States)

    Quintavalle, Gabriele; Riccardi, Federica; Rivolta, Gianna Franca; Martorana, Davide; Di Perna, Caterina; Percesepe, Antonio; Tagliaferri, Annarita

    2017-08-01

    Congenital factor VII (FVII) deficiency is a rare bleeding disorder caused by mutations in F7 gene with autosomal recessive inheritance. A clinical heterogeneity with poor correlation with FVII:C levels has been described. It was the objective of this study to identify genetic defects and to evaluate their relationships with phenotype in a large cohort of patients with FVII:CF7 mutations and three polymorphic variants and classified according to recently published clinical scores. Forty out of 123 patients (33 %) were symptomatic (43 bleedings). A severe bleeding tendency was observed only in patients with FVII:CF7 international databases. Most mutations (62 %) were missense, large deletions were 6.2 %. Compound heterozygotes/homozygotes for mutations presented lower FVII:C levels compared to the other classes (Chi(2)=43.709, pF7 genotypic groups (Chi(2)=72.289, pF7 mutation and/or polymorphisms and FVII:C levels, without a direct link between FVII:C and bleeding tendency. The results suggest that large deletions are underestimated and that they represent a common mechanism of F7 gene inactivation which should always be investigated in the diagnostic testing for FVII deficiency.

  11. Characterisation of a large complex intragenic re-arrangement in the FVII gene (F7) avoiding misdiagnosis in inherited factor VII deficiency.

    Science.gov (United States)

    Giansily-Blaizot, Muriel; Thorel, Delphine; Khau Van Kien, Philippe; Behar, Catherine; Romey, Marie-Catherine; Mugneret, Francine; Schved, Jean-François; Claustres, Mireille

    2007-08-01

    Inherited factor VII (FVII) deficiency is a rare autosomal recessive bleeding disorder mostly caused by point mutations. Large genomic re-arrangements at F7 locus could account for a fraction of mutant alleles that remain unidentified after DNA sequencing, because they escape conventional polymerase chain reaction (PCR)-based techniques. We report the first systematic screening of F7 for large re-arrangements, by semi-quantitative multiplex PCR of fluorescent fragments targeting the 9 exons and the promoter region. A well-characterised cohort of 43 unrelated patients either apparently homozygous for a F7 point mutation or carrying at least one unidentified F7 mutant allele participated in this study. Two large F7 re-arrangements were identified in two FVII-deficient pedigrees, including a discontinuous deletion involving two distinct portions of F7 whose proximal and distal end junctions were characterised. A simple and efficient method for the routine detection of gross alterations of F7, which accounted for 2.3% of mutant alleles in our sample, is now available in inherited FVII deficiency. This test should complement conventional PCR-based techniques not only in unsolved cases, but also where inheritance pattern analysis is not achievable.

  12. A massive transfusion protocol incorporating a higher FFP/RBC ratio is associated with decreased use of recombinant activated factor VII in trauma patients.

    Science.gov (United States)

    Tan, Josenia N M; Burke, Peter A; Agarwal, Suresh K; Mantilla-Rey, Nelson; Quillen, Karen

    2012-04-01

    We implemented a protocol incorporating a higher fresh frozen plasma (FFP)/RBC ratio for the management of trauma patients requiring massive transfusion in 2007. This study aims to identify issues that affected the effective deployment of the massive transfusion protocol (MTP) and compare outcome variables with a historic cohort. Data from 49 trauma patients who received at least 10 units of packed RBCs within 24 hours were analyzed and compared with a historic massively transfused cohort who had received recombinant activated factor VII (rFVIIa). Of the patients, 28 received an FFP/RBC ratio of 1:1 to 1:2; 12 received a lower ratio of 1:2 to 1:4; 3 received more than 1:1 and 6 had less than 1:4. Compared with the historic cohort, the 1:1-1:2 group received significantly fewer blood components and did not require rescue rFVIIa. An MTP incorporating a higher FFP/RBC ratio of 1:1 to 1:2 is associated with decreased use of blood components and may obviate the need for rFVII.

  13. Proteína recombinante para el diagnostico del síndrome de inmunodeficiencia adquirida (SIDA)

    OpenAIRE

    2004-01-01

    Proteína recombinante para el diagnóstico del síndrome de inmunodeficiencia adquirida (SIDA). La proteína recombinante comprende una proteína que presenta actividad b-galactosidasa en la que se ha insertado al menos una secuencia de aminoácidos que contiene al menos un epítopo del virus de la inmunodeficiencia humana (VIH). La presente invención se refiere a una proteína recombinante de fusión que tiene aplicación para el diagnóstico del síndrome de la inmunodeficiencia adquirida.

  14. Producción de proteínas recombinantes en Bacillus megaterium: estado del arte

    OpenAIRE

    Keyla Tortoló-Cabañas; Antonio Bell-García

    2015-01-01

    Se recoge una actualización del estado del arte del uso de Bacillus megaterium como hospedero para la expresión de proteínas recombinantes, las características de este microorganismo, así como las consideraciones generales y algunos aspectos a tener en cuenta.

  15. Haplotype and genotype effects of the F7 gene on circulating factor VII, coagulation activation markers and incident coronary heart disease in UK men.

    Science.gov (United States)

    Ken-Dror, G; Drenos, F; Humphries, S E; Talmud, P J; Hingorani, A D; Kivimäki, M; Kumari, M; Bauer, K A; Morrissey, J H; Ireland, H A

    2010-11-01

    Evidence for the associations of single nucleotide polymorphisms (SNPs) in the F7 gene and factor (F)VII levels and with risk of coronary heart disease (CHD) is inconsistent. We examined whether F7 tagging SNPs (tSNPs) and haplotypes were associated with FVII levels, coagulation activation markers (CAMs) and CHD risk in two cohorts of UK men. Genotypes for eight SNPs and baseline levels of FVIIc, FVIIag and CAMs (including FVIIa) were determined in 2773 healthy men from the Second Northwick Park Heart Study (NPHS-II). A second cohort, Whitehall II study (WH-II, n = 4055), was used for replication analysis of FVIIc levels and CHD risk. In NPHS-II the minor alleles of three SNPs (rs555212, rs762635 and rs510317; haplotype H2) were associated with higher levels of FVIIag, FVIIc and FVIIa, whereas the minor allele for two SNPs (I/D323 and rs6046; haplotype H5) was associated with lower levels. Adjusted for classic risk factors, H2 carriers had a CHD hazard ratio of 1.34 [95% confidence interval (CI): 1.12-1.59; independent of FVIIc], whereas H5 carriers had a CHD risk of 1.29 (95% CI: 1.01-1.56; not independent of FVIIc) and significantly lower CAMs. Effects of haplotypes on FVIIc levels were replicated in WH-II, as was the association of H5 with higher CHD risk [pooled-estimate odds ratio (OR) 1.16 (1.00-1.36), P = 0.05], but surprisingly, H2 exhibited a reduced risk for CHD.  tSNPs in the F7 gene strongly influence FVII levels. The haplotype associated with low FVIIc level, with particularly reduced functional activity, was consistently associated with increased risk for CHD, whereas the haplotype associated with high FVIIc level was not. © 2010 International Society on Thrombosis and Haemostasis.

  16. Critical appraisal of the role of recombinant activated factor VII in the treatment of hemophilia patients with inhibitors

    Directory of Open Access Journals (Sweden)

    Ampaiwan Chuansumrit

    2010-03-01

    Full Text Available Ampaiwan Chuansumrit1, Pantep Angchaisuksiri2, Nongnuch Sirachainan11Departments of Pediatrics and 2Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University,  Bangkok, ThailandAbstract: Hemophilia patients with inhibitors faced the constraint of inadequate treatment for several years before the era of recombinant factor VIIa (rFVII. Initially, rFVIIa was used in the compassionate-use programs. After a worldwide license was issued, more than 1.5 million doses were administered. Bleeding of joints and muscles was controlled effectively by means of an early home treatment program, with either a standard dose of 90 μg/kg every 2 to 3 hours for a few doses or a single dose of 270 μg/kg. For more serious bleeding episodes or minor surgery, an initial dose of 90 μg/kg was given every 2 hours for 24 to 48 hours followed by increased intervals of 3 to 6 hours according to the severity of bleeding and efficacy of bleeding control. In cases of major surgery such as orthopedic procedures, the same regimen can be applied except for a higher initial dose of 120 to 180 μg/kg. However, increasing the dose should be considered if there are unexpected bleeding complications since the half-life and clearance of rFVIIa differ between individuals. In addition, prophylaxis is administered to a small number of patients. Finally, the reported thromboembolic events found in hemophilia patients with inhibitors receiving rFVIIa are extremely low, much less than 1%.Keywords: bleeding disorder, hemophilia, inhibitor, NovoSeven, recombinant factor VIIa

  17. Ineffective off-label use of recombinant activated factor VII in a case of bone-marrow transplantation-related gastrointestinal bleeding

    Directory of Open Access Journals (Sweden)

    Wiedermann CJ

    2006-01-01

    Full Text Available Abstract Background For patients with a normal coagulation system, who experience serious bleeding, sound evidence for recombinant activated factor VII (rFVIIa as an effective haemostatic agent is only scarcely available so far from controlled clinical trials. In systematic reviews on the clinical use of rFVIIa, treatment failures were only rarely reported. Case presentation We present a 45-year old, Caucasian male with persistent intestinal bleeding due to enterocolitis associated with cytomegalovirus infection and acute graft-versus-host-disease. He had received allogeneic peripheral blood stem cell transplantation from an unrelated HLA-identical donor because of chronic myelogenous leukaemia diagnosed two years earlier. Bleeding started at day 18 after transplantation with bloody diarrhea, which was treated with multiple transfusions of fresh frozen plasma, platelet, and red blood cell concentrates, and continued relentlessly, despite all efforts, including continued transfusions, high-dose prednisolone, broad antibiotic and antiviral coverage, and tranexamic acid. Recombinant FVIIa was started at boluses of 90–120 μg/kg every 4–8 hours. Despite more than 10 doses, recurrent severe bleeding progressed to refractory shock, multiorgan failure and death. Conclusions Little can be concluded from single case reports of clinical improvement, because publication bias in favour of positive effects is likely. Our case suggests that rFVIIa is not a panacea, in particular for severe bleeding after bone-marrow transplantation. As long as rigorous, controlled studies or comprehensive registries are lacking, conventional interventions remain the standard of care in non-haemophilic patients with severe bleeding.

  18. Treatment of bleeding in acquired hemophilia A with the proper administration of recombinant activated factor VII: single-center study of 7 cases

    Science.gov (United States)

    Saito, Makoto; Kanaya, Minoru; Izumiyama, Koh; Mori, Akio; Irie, Tatsuro; Tanaka, Masanori; Morioka, Masanobu; Ieko, Masahiro

    2016-01-01

    Recombinant activated factor VII (rFVIIa) is the bypassing agent used in the first-line hemostatic therapy for acquired hemophilia A (AHA); however, the occurrence of thrombotic complications in rFVIIa-treated AHA patients was recently reported to be 2.9–6.5%. Therefore, the investigation of the proper administration of rFVIIa for AHA is needed. In the present study, we retrospectively investigated the clinical features of AHA with regards to the use of rFVIIa (presence or absence of use and total amount) in 7 AHA patients encountered in our department for 7 years between January 2008 and December 2014. Ages were 63–89 years old (median: 79 years old), and there were 5 male and 2 female patients. The coexistence of cardiovascular risk factors and arteriosclerotic diseases, such as hypertension, diabetes mellitus, and cerebral infarction were present in 6 patients. Anemia progressed to less than 7 g/dL of hemoglobin and required red blood cell transfusion in 5 patients, showing “severe” hemorrhage. Factor VIII inhibitors were removed by immunological treatments in 6 patients. As a hemostatic therapy, rFVIIa was used in 4 patients. rFVIIa was not administered or was administered at a very low dose (20 mg) to 3 and 1 patient, respectively, and bleeding stopped as inhibitor titers decreased and disappeared in these patients. Inhibitors did not disappear in 1 patient and the control of hemostasis became poor and was accompanied by intestinal hemorrhage. Although a large amount of rFVIIa (265 mg in total) was administered, the patient bled to death. Therefore, bleeding may be stopped without the administration of rFVIIa in some AHA cases, while the dose of rFVIIa is not necessarily related to hemostatic effects in other cases. Since the main aim of AHA treatments is the removal of inhibitors, caution is needed to ensure that more than the necessary amount of rFVIIa is not administered. PMID:27843336

  19. [Study of the polymorphism R353Q in the coagulation factor VII gene and the N700S in the thrombospondin-1 gene in young patients with acute myocardial infarction].

    Science.gov (United States)

    Valades-Mejía, María Guadalupe; Domínguez-López, María Lilia; Aceves-Chimal, José Luis; Miranda, Alfredo Leaños; Majluf-Cruz, Abraham; Isordia-Salas, Irma

    2014-01-01

    Antecedentes: el infarto agudo de miocardio es la principal causa de morbilidad y mortalidad en el mundo, y resulta de la combinación de factores modificables y genéticos. Se ha propuesto que el polimorfismo R353Q en el gen del factor VII de la coagulación representa un factor protector en contra del infarto agudo de miocardio, mientras que el polimorfismo N700S en el gen de la trombospondina-1 (TSP- 1) incrementa el riesgo; sin embargo, los resultados aún suscitan controversia. Objetivo: determinar la posible asociación de los polimorfismos R353Q y del N700S con el infarto agudo de miocardio en pacientes mexicanos menores de 45 años. Material y métodos: estudio de casos y controles que incluyó 252 pacientes con diagnóstico de infarto agudo de miocardio y 252 individuos aparentemente sanos sin antecedentes de enfermedad coronaria, pareados por edad y sexo. Los polimorfismos R353Q N700S se determinaron en todos los participantes por medio de PCR-RFLP. Resultados: no se observó diferencia estadística en la distribución genotípica del polimorfismo R353Q del FVII entre los grupos con infarto agudo de miocardio y el grupo control (p = 0.06). Se encontró una distribución genotípica similar del polimorfismo N700S en ambos grupos (p = 0.50). Se identificaron como factores de riesgo independiente para infarto agudo de miocardio: hipertensión arterial, diabetes mellitus, antecedentes heredofamiliares para enfermedad coronaria y dislipidemia. Conclusiones: los polimorfismos R353Q y N700S no representan un factor protector o de riesgo, respectivamente, para infarto agudo de miocardio en pacientes jóvenes mexicanos. Palabras clave: factor VII de la coagulación, trombospondina-1, infarto agudo de miocardio, polimorfismo.

  20. Intelsat VII planning and evolution

    Science.gov (United States)

    Nadkarni, P.; Neyret, P.; Allnutt, J.; Chidambaram, T.

    This paper describes the evolution of the Intelsat VII concept from among a number of spacecraft concepts considered in the planning process. The considerations of greatest importance in this evolution are examined, including the compatibility with small earth stations, available digital services and circuit multiplication techniques, schedule considerations, launch vehicle considerations, and operational flexibility. The roles of demand analysis and of architecture selection in the development of the Intelsat VII concept are addressed.

  1. Somatotropina bovina recombinante en sincronización de estros y prolificidad de ovejas Pelibuey

    OpenAIRE

    2014-01-01

    Con la finalidad de estudiar si la administración de la Somatotropina Bovina Recombinante (bST) dos días antes del retiro del progestágeno, tiene efecto en la sincronización de estros, inicio del estro, fertilidad, prolificidad y tipo de parto en ovejas Pelibuey, a 180 ovejas de 4,0 ± 0,32 años de edad, se les inserto un dispositivo intravaginal impregnado con progesterona (CIDR P4 0,3 g), por nueve días. Dos días antes del retiro del dispositivo, las ovejas se asignaron aleatoriamente a uno ...

  2. Vectores recombinantes basados en el virus Vaccinia modificado de Ankara (MVA) como vacunas contra la leishmaniasis

    OpenAIRE

    Pérez Jiménez, Eva; Larraga, Vicente; Esteban, Mariano

    2005-01-01

    Vectores recombinantes basados en el virus vaccinia modificado de Ankara (MVA) como vacunas contra la leishmaniasis. Los vectores de la invención contienen secuencias codificantes de la proteína LACK, preferentemente insertadas en el locus de hemaglutinina del virus y bajo el control de un promotor que permite su expresión a lo largo del ciclo de infección del virus. Son vectores seguros, estables, que dan lugar a una potente respuesta inmune que confiere protección frente a la leishmaniasis,...

  3. Altered lumbar spine structure, biochemistry and biomechanical properties in a canine model of mucopolysaccharidosis type VII

    Science.gov (United States)

    Smith, Lachlan J; Martin, John T; Szczesny, Spencer E; Ponder, Katherine P; Haskins, Mark E; Elliott, Dawn M

    2010-01-01

    Mucopolysaccharidosis VII (MPS VII) is a lysosomal storage disorder characterized by a deficiency in β-glucuronidase activity, leading to systemic accumulation of poorly degraded glycosaminoglycans (GAG). Along with other morbidities, MPS VII is associated with paediatric spinal deformity. The objective of this study was to examine potential associations between abnormal lumbar spine matrix structure and composition in MPS VII, and spine segment and tissue-level mechanical properties, using a naturally occurring canine model with a similar clinical phenotype to the human form of the disorder. Segments from juvenile MPS VII and unaffected dogs were allocated to: radiography, gross morphology, histology, biochemistry, and mechanical testing. MPS VII spines had radiolucent lesions in the vertebral body epiphyses. Histologically, this corresponded to a GAG-rich cartilaginous region in place of bone, and elevated GAG staining was seen in the annulus fibrosus. Biochemically, MPS VII samples had elevated GAG in the outer annulus fibrosus and epiphyses, low calcium in the epiphyses, and high water content in all regions except the nucleus pulposus. MPS VII spine segments had higher range of motion and lower stiffness than controls. Endplate indentation stiffness and failure loads were significantly lower in MPS VII samples, while annulus fibrosus tensile mechanical properties were normal. Vertebral body lesions in MPS VII spines suggest a failure to convert cartilage to bone during development. Low stiffness in these regions likely contributes to mechanical weakness in motion segments and is a potential factor in the progression of spinal deformity. PMID:19918911

  4. Evaluación inmunológica de proteínas recombinantes de fusión de dengue 1 y dengue 2 en monos

    OpenAIRE

    Bernardo Reyes, Lídice

    2010-01-01

    El desarrollo de subunidades recombinantes que contienen al dominio III de la proteína de la envoltura (E) de los virus del dengue (DEN) es una alternativa para el desarrollo de una vacuna segura contra esta entidad. En este trabajo caracterizamos la respuesta inmune inducida en monos por proteínas recombinantes de fusión de DEN 1 y DEN 2 que contienen al dominio III de la proteína E fusionado a la proteína P64k de Neisseria meningitidis. Se demostró que la proteína recombinante P64k-domin...

  5. Ajateenistust alustab ka viis neidu

    Index Scriptorium Estoniae

    2013-01-01

    Tuleval nädalal alustab ajateenistust üle 1600 noormehe, lisaks alustab juulis Kuperjanovi jala­väepataljonis vabatahtlikuna ajateenistust ka viis neidu. Juulikuise teenistusse kutsumisega alustavad teenistust ajateenijad, kelle ametikoht eeldab 11-kuulist ajateenistust ehk autojuhid, erialaspet­sialistid, allohvitserid ja reservrühmaülemad

  6. Anticuerpos policlonales contra la proteína recombinante NS3 del virus del dengue

    Directory of Open Access Journals (Sweden)

    Liliana Morales

    2017-01-01

    Resultados. Los anticuerpos producidos fueron útiles en ensayos de Western blot e inmunofluorescencia y se reportó por primera vez un anticuerpo policlonal anti-NS3 que permitió la inmunoprecipitación de la proteína viral y la detecta con Western blot sin necesidad de inducir sobreexpresión de NS3 o de usar extractos de células marcados metabólicamente con radioisótopos. Conclusión. Las proteínas recombinantes expresadas y los anticuerpos producidos constituyen herramientas valiosas para estudiar procesos infecciosos del DENV que involucren a la proteína NS3 y evaluar pruebas dirigidas a interferir las funciones de esta proteína.

  7. PRODUCCIÓN DE PROTEÍNAS RECOMBINANTES EN Escherichia coli

    OpenAIRE

    Lara, Alvaro R.

    2011-01-01

    La producción de proteínas recombinantes (PR) es una de las aportaciones más importantes de la biotecnología moderna. El hospedero bacteriano más importante para la producción de PR es Escherichia coli, aunque otros como Bacillus subtilis y Bacillus megaterium están cobrando cada vez más importancia. Debido a su impacto económico y en el sector de la salud humana, el presente trabajo se enfocará principalmente a la producción de PR terapéuticas en E. coli. Se abordarán en general los aspectos...

  8. Positivity VII (Zaanen Centennial Conference)

    CERN Document Server

    Pagter, Ben; Gaans, Onno; Veraar, Mark

    2016-01-01

    This book presents the proceedings of Positivity VII, held from 22-26 July 2013, in Leiden, the Netherlands. Positivity is the mathematical field concerned with ordered structures and their applications in the broadest sense of the word. A biyearly series of conferences is devoted to presenting the latest developments in this lively and growing discipline. The lectures at the conference covered a broad spectrum of topics, ranging from order-theoretic approaches to stochastic processes, positive solutions of evolution equations and positive operators on vector lattices, to order structures in the context of algebras of operators on Hilbert spaces. The contributions in the book reflect this variety and appeal to university researchers in functional analysis, operator theory, measure and integration theory and operator algebras. Positivity VII was also the Zaanen Centennial Conference to mark the 100th birth year of Adriaan Cornelis Zaanen, who held the chair of Analysis in Leiden for more than 25 years and was ...

  9. Produção de proteínas recombinantes por plantas transgénicas

    OpenAIRE

    Filipe, Ana Patrícia da Costa

    2015-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz O desenvolvimento da tecnologia de ADN recombinante permite a introdução de genes exógenos de interesse em células de diferentes sistemas de expressão com o fim de obtenção de características desejáveis. As plantas transgénicas têm sido desenvolvidas para o melhoramento agrícola, para a produção de alimentos funcionais, e mais recentemente, para a produção de proteínas recombinantes. A utiliza...

  10. Uso da eritropoetina recombinante humana no tratamento da anemia associada à insuficiência renal crônica

    OpenAIRE

    Carvalho, Mauricio de

    2013-01-01

    Resumo: A anemia acompanha a insuficiencia renal cronica e esta presente na maioria dos pacientes em dialise. O objetivo deste estudo foi analisar o efeito de seis meses de tratamento com a eritropoetina recombinante humana (EPO-rHu) na anemia associada a insuficiencia renal cronica terminal em vinte pacientes mantidos em programa de hemodialise. Os criterios de inclusao foram: pacientes que estivessem em hemodialise por pelo menos 3 meses, com idade maior que 18 ou menor que 70 anos; hematoc...

  11. Inmunogenicidad de una vacuna recombinante anti-HBs en trabajadores de la salud, del Instituto de Medicina Legal de Colombia

    Directory of Open Access Journals (Sweden)

    Heber Siachoque

    2007-12-01

    Full Text Available Objetivo: Evaluar la inmunogenicidad de la vacuna recombinante Hepavax-Gene para hepatitis B desde la última dosis administrada en trabajadores del INML.Materiales y métodos: Estudio de corte transversal en 603 trabajadores de la salud con mínimo 3 dosis de vacuna recombinante (0, 1, 6 meses donde se midieron los niveles de anticuerpos anti-HBs con la técnica de ELISA entre diciembre de 2000 y enero de 2001 desde la aplicación de la última dosis de la vacuna, que varió entre 1 y 6 años.Resultados: El grupo de estudio lo conformaron 344 hombres y 259 mujeres, con un promedio de edad de 38.8±7.3 años. El nivel de protección fue 90.7% (>10 U/l que disminuyó significativamente con el tiempo de aplicación de la última dosis de la vacuna (p45 (RRI=3.58, IC 95%:1.83, 6.99 con respecto a <40 años. Conclusión: La vacuna recombinante Hepavax-Gene anti-HBs tiene alta efectividad en los trabajadores de la salud (90.7% aunque presenta disminución de protección a mayor tiempo de aplicación de la última dosis y al aumentar la edad del trabajador.

  12. FAKTOR-FAKTOR YANG MEMENGARUHI PRODUKSI GULA PTPN VII (PERSERO

    Directory of Open Access Journals (Sweden)

    Rizky Savitri

    2014-09-01

    Full Text Available ABSTRACTThis study aimed to analyze the influence of the level of sugar cane yield, labor, production process period, and supporting materials on the production of PTPN VII (Persero PG. Cinta Manis, as well as analyzing the elasticity of production and business scale  of PTPN VII (Persero PG. Cinta Manis. The method used to analyze the data were quantitative analysis of Cobb Douglas production function with ordinary least square method to observe variable influences of PG. Cinta Manis sugar production. Cobb Douglas estimation results indicate that the level of sugar cane yield, labor, production process period, and supporting materials affect sugar production in PTPN VII (Persero PG. Cinta Manis. Production elasticity of each production factor shows that the use of production factors is not yet optimal, while the production scale of the company is Increasing Return to Scale (IRTS in which the proportion of additional input will produce output with the larger proportion. Accordingly, company should utilize the factors of production optimally, improve the quality of human resources with various activities to improve skills, increase the productivity of land by having land intensification, and optimize milling period by improving the quality of machine.Keywords: PG. Cinta Manis, increasing return to scale, Cobb Douglas function, PTPN VIIABSTRAKPenelitian ini bertujuan menganalisis pengaruh tingkat rendemen, tenaga kerja, bahan pembantu dan lama lama giling terhadap produksi gula PTPN VII (Persero PG. Cinta Manis, sekaligus menganalisis elastisitas produksi dan skala usaha PTPN VII (Persero PG. Cinta Manis. Metode analisis data yang digunakan adalah  analisis kuantitatif. Analisis kuantitatif pada penelitian ini menggunakan fungsi produksi cobb douglas dengan metode ordinary least square untuk melihat pengaruh variabel-variabel yang telah ditentukan terhadap produksi gula PG. Cinta Manis. Hasil estimasi cobb douglas menunjukkan  bahwa tingkat

  13. Preface: STATPHYS-Kolkata VII

    Science.gov (United States)

    Bhattacharjee, Jayanta Kumar; Chakrabarti, Bikas K.; Inoue, Jun-Ichi; Sen, Parongama

    2011-04-01

    In the past two decades, a series of international conferences on Statistical Physics, going by the name Statphys Kolkata, have been organized in Kolkata (previously Calcutta) at roughly three-year intervals, the first one being held in 1992-93. The seventh of this series, Statphys Kolkata VII (http://www.saha.ac.in/cmp/stat.vii/index.php) was held from 26-30 November 2010. This meeting was organized as part of the Silver Jubilee Celebration of the Satyendra Nath Bose National Centre for Basic Sciences, Kolkata, in collaboration with the Saha Institute of Nuclear Physics, Kolkata. In Statphys Kolkata VII, a few topics of current interest such as Collective behavior and emergent phenomena, Systems far from equilibrium, Soft matter, and Quantum critical phenomena were given special emphasis, while various other issues of Statistical Physics were also addressed. We were happy to note that the conference attracted a large number of participants, and the talk and poster sessions generated a lot of discussions, arguments and collaborations. The articles appearing in this proceedings are based on the invited talks and selected poster presentations. We would like to thank the Journal of Physics Conference Series, IOP, for publishing the proceedings of the conference, and the referees for their prompt and active support. The proceedings of the earlier Statphys Kolkata conferences have appeared in Physica A, vol 384 (2007); Physica A, vol 346 (2005); Physica A, vol 318 (2003); Physica A, vol 270 (1999); Physica A, vol 224 (1996); and Physica A, vol 186 (1992). We would like to take this opportunity to thank all the members of the organizing committee (especially Dr Anjan Kumar Chandra for extensive all-round help), and acknowledge the Centre for Applied Mathematics and Computational Science (CAMCS, Saha Institute of Nuclear Physics, Kolkata) and Satyendra Nath Bose National Centre for Basic Sciences, Kolkata, for their financial support. Jayanta Kumar Bhattacharjee, Bikas K

  14. Dietary factor VII activation does not increase plasma concentrations of prothrombin fragment 1+2 in patients with stable angina pectoris and coronary atherosclerosis

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Münster, A M; Marckmann, P

    2000-01-01

    tissue factor, the cofactor of FVII, to circulating blood; therefore, thrombin formation in response to FVII activation is more likely to occur in such patients. This hypothesis was tested in a randomized crossover study of 30 patients (aged 43 to 70 years) with stable angina pectoris...

  15. Association of protein Z and factor VII gene polymorphisms with risk of cerebral hemorrhage: a case–control and a family-based association study in a Chinese Han pulation

    Indian Academy of Sciences (India)

    YI ZENG; LE ZHANG; ZHIPING HU; QIDONG YANG; MINGMING MA; BAOQIONG LIU; JIAN XIA; HONGWEI XU; YUNHA I LIU; XIAOPING DU

    2016-06-01

    Protein Z (PZ) and factor (F) VII are two important factors in the clotting pathway which have similar structure, linkedfunction and nearby gene sites. The aims of this study were to investigate whether the common variants of PZ and FVII genesare associated with the risk of cerebral hemorrhage (CH) and to explore the combined effects of PZ and FVII polymorphismsfor CH risk. We performed genotyping analysis for two single-nucleotide polymorphisms (SNPs) of FVII (rs510317 andrs6046) and three SNPs of PZ (rs2273971, rs3024718 and rs3024731) both in a population-based case–control study andin a family-based association study. Case–control analysis found no evidence of significant association. But family-basedassociation study revealed that the G allele of PZ rs2273971, and three haplotypes carrying the ‘G’ allele of PZ rs2273971:haplotype GA, CG and CGA of PZ and FVII genes, all had a significant effect on CH susceptibility (Z =1.882,P =0.049;Z =1.922,P =0.044; Z =1.826,P =0.047; Z =1.977,P =0.048, respectively). While, the A allele of PZ rs2273971, andfour haplotypes carrying or crossing the ‘A’ allele of PZ rs2273971: haplotypes CA, ACAA, ACAT and ACAAT of PZ andFVII genes, may confer protection against CH (Z =−1.882,P =0.049; Z =−2.000,P =0.045; Z =−2.319,P =0.020;Z =−2.002,P =0.045; Z =−2.015,P =0.043, respectively). This is a first family-based association study providing geneticevidences that PZ and FVII genes, especially PZ rs2273971 are involved in the development of CH in Han-Chinese families.

  16. Association of protein Z and factor VII gene polymorphisms with risk of cerebral hemorrhage: a case-control and a family-based association study in a Chinese Han population.

    Science.gov (United States)

    Zeng, Yi; Zhang, Le; Hu, Zhiping; Yang, Qidong; Ma, Mingming; Liu, Baoqiong; Xia, Jian; Xu, Hongwei; Liu, Yunhai; Du, Xiaoping

    2016-06-01

    Protein Z (PZ) and factor (F) VII are two important factors in the clotting pathway which have similar structure, linked function and nearby gene sites. The aims of this study were to investigate whether the common variants of PZ and FVII genes are associated with the risk of cerebral hemorrhage (CH) and to explore the combined effects of PZ and FVII polymorphisms for CH risk. We performed genotyping analysis for two single-nucleotide polymorphisms (SNPs) of FVII (rs510317 and rs6046) and three SNPs of PZ (rs2273971, rs3024718 and rs3024731) both in a population-based case-control study and in a family-based association study. Case-control analysis found no evidence of significant association. But family-based association study revealed that the G allele of PZ rs2273971, and three haplotypes carrying the 'G' allele of PZ rs2273971: haplotype GA, CG and CGA of PZ and FVII genes, all had a significant effect on CH susceptibility (Z = 1.882, P = 0.049; Z = 1.922, P = 0.044; Z = 1.826, P = 0.047; Z = 1.977, P = 0.048, respectively). While, the A allele of PZ rs2273971, and four haplotypes carrying or crossing the 'A' allele of PZ rs2273971: haplotypes CA, ACAA, ACAT and ACAAT of PZ and FVII genes, may confer protection against CH (Z= -1.882, P = 0.049; Z= -2.000, P = 0.045; Z= -2.319, P = 0.020; Z= -2.002, P = 0.045; Z= -2.015, P = 0.043, respectively). This is a first family-based association study providing genetic evidences that PZ and FVII genes, especially PZ rs2273971 are involved in the development of CH in Han-Chinese families.

  17. Assessment of individual dose utilization vs. physician prescribing recommendations for recombinant activated factor VII (rFVIIa) in paediatric and adult patients with congenital haemophilia and alloantibody inhibitors (CHwI): the Dosing Observational Study in Hemophilia (DOSE).

    Science.gov (United States)

    Gruppo, R A; Kessler, C M; Neufeld, E J; Cooper, D L

    2013-07-01

    Recent data from the Dosing Observational Study in Hemophilia diary study has described home treatment with recombinant activated factor VII (rFVIIa) in congenital haemophilia with inhibitors (CHwI). The current analysis compares prescribed and patient/caregiver-reported rFVIIa administration in paediatric and adult CHwI patients in this study. Patients with ≥ 4 bleeding episodes within a 3-month period prescribed rFVIIa as first-line therapy for bleeding episodes were eligible. Patients/caregivers completed a diary for ≥ 90 days or until the patient experienced four bleeds. Initial, total and mean rFVIIa doses reported for each bleeding episode were calculated and compared with the physician-prescribed doses. Of 52 enrolled patients (25 children; 27 adults), 39 (75%) completed the study. Children and adults had similar mean durations of bleeding episodes. Both patient groups were administered higher initial rFVIIa doses for joint bleeds than prescribed: median (range) 215.2 (74.1-400.0) mcg kg(-1) vs. 200.0 (61.0-270.0) mcg kg(-1) for children, and 231.3 (59.3-379.7) mcg kg(-1) vs. 123.0 (81.0-289.0) mcg kg(-1) for adults. The median infused dose for joint bleeds was higher in adults than children (175.2 vs. 148.0 mcg kg(-1) ), but children received significantly more doses per joint bleed than adults (median 6.5 vs. 3.0). The median total dose per joint bleed was higher in children than adults (1248.7 vs. 441.6). For children and adults, both initial and additional doses administered for bleeds were higher than prescribed. Children received higher total doses per bleed due to an increased number of infusions per bleed.

  18. Método de producción de repertorios complejos de moléculas recombinantes

    OpenAIRE

    Orzáez, Diego; Julve, José Manuel; Granell, Antonio; Sarrion-Perdigones, Alejandro; Gutiérrez Cabrera, Carlos

    2012-01-01

    La presente invención describe un método de producción de repertorios complejos de moléculas recombinantes de forma consistente y reproducible, mediante la generación de forma transitoria de plantas multi-transgénicas que dan lugar a mosaicos somáticos inducidos por replicones virales que se excluyen entre sí. La presente invención también hace referencia a la planta multi-transgénica o un fragmento de la misma así obtenida, así como a los extractos o fracciones pur...

  19. Tecnología recombinante y el control de la rabia en animales domésticos y salvajes

    OpenAIRE

    Gallo, Guillermo G.

    1999-01-01

    La rabia es todavía un importante problema público en muchas partes del mundo. En 1993 y 1994 la Organización Mundial de la Salud O.M.S. comunicó más de 30.000 casos de rabia humana cada año. Las vacunas de rabia convencionales por vía parenteral han demostrado alta eficacia y seguridad y estas vacunas controlaron la rabia en animales domésticos. Sin embargo, la erradicación no es completa en los reservorios salvajes. Un recombinante del virus de vaccinia fue construido al insertar un gen de ...

  20. Eficacia de la eritropoyetina recombinante humana, sin albúmina, cubana en pacientes en hemodiálisis iterada

    OpenAIRE

    Pérez-Oliva Díaz, Jorge Francisco

    2011-01-01

    La corrección de la anemia renal con eritropoyetina recombinante humana cambió la calidad de vida del enfermo en hemodiálisis. Un producto nacional se emplea desde 1998, en una formulación con albúmina como estabilizante con demostrada eficacia y seguridad. El objetivo de este ensayo clínico fue evaluar un nuevo biosimilar, cubano, sin albúmina, su eficacia y seguridad Método: ensayo clínico, controlado, monocéntrico, a doble ciega y aleatorizado, en enfermos hemodializados en el Instituto de...

  1. Somatotropina bovina recombinante (rbST) en la sincronización de estros y prolificidad de ovejas pelibuey.

    OpenAIRE

    2012-01-01

    El objetivo del presente estudio fue determinar si la administración de la Somatotropina Bovina Recombinante (rbST) dos días antes del retiro del progestágeno, influye en la sincronización de estros y prolificidad en ovejas Pelibuey. Se utilizaron 180 ovejas de 4±0.32 años de edad, a las cuales se les inserto un dispositivo intravaginal impregnado con progesterona (CIDR P4 0.3 g) por nueve días. Dos días antes del retiro del dispositivo, las ovejas se asignaron aleatoriamente a tres tratamie...

  2. Extração de pró-insulina humana recombinante produzida em sementes de milho transgênico

    OpenAIRE

    Cristiane Sanchez Farinas

    2004-01-01

    Resumo: Bioprodutos de aplicação fannacêutica têm sido produzidos em sistemas transgênicos diversos, incluindo células de animais, bactérias e leveduras. No futuro, é esperado que a demanda por novas proteínas terapêuticas cresça substancialmente, sendo que o uso de plantas como bioITeatores tem se mostrado como uma das alternativas economicamente mais atraentes para a produção de proteínas recombinantes em larga escala. O objetivo deste trabalho foi estudar o processo de extração da pró-insu...

  3. Estreptoquinasa recombinante en pacientes con infarto agudo del miocardio. Cardiocentro de Camaguey, 2008

    Directory of Open Access Journals (Sweden)

    Héctor Mariño Cano

    2015-12-01

    Full Text Available Se realizó un estudio descriptivo longitudinal, con el objetivo de valorar la eficacia de la utilización de la Estreptoquinasa Recombinante en pacientes con Infarto Agudo de Miocardio (IMA en el Cardiocentro del Hospital Provincial Docente “Manuel Ascunce Doménech” en la provincia de Camagüey, en el período comprendido de enero a diciembre del 2008. El universo estuvo constituido por 200 pacientes, a los que se les diagnosticó Infarto Agudo de Miocardio y la muestra quedó formada por 80 pacientes que recibieron tratamiento trombolítico, las historias clínicas de los pacientes fueron la principal fuente de obtención de la información, utilizando  las  siguientes  variables: resultados criterio de perfusión, evolución clínica, criterio de reacciones adversas y la influencia que ejerce el tiempo puerta-aguja,  así como los cuidados de enfermería. Al terminar se observó que  el criterio de repercusión de mayor incidencia fue el eléctrico; la mayor cantidad de pacientes tuvieron buena evolución clínica; la hipotensión fue la reacción adversa más frecuente; el tiempo de puerta-aguja  que más incidió  fue el menor, de 3h; a todos los pacientes diagnosticados con infarto, que recibieron tratamiento trombolítico, se les  brindaron  cuidados de enfermería.

  4. Silence of Synaptotagmin VII inhibits release of dense core vesicles in PC12 cells

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Synaptotagmin VII (Syt VII), which has a higher Ca2+ affinity and slower disassembly kinetics with lipid than Syt I and Syt IX, was regarded as being uninvolved in synaptic vesicle (SV) exocytosis but instead possibly as a calcium sensor for the slower kinetic phase of dense core vesicles (DCVs) release. By using high temporal resolution capacitance and amperometry measurements, it was demonstrated that the knockdown of endogenous Syt VII attenuated the fusion of DCV with the plasma membrane, reduced the amplitude of the exocytotic burst of the Ca2+-triggered DCV release without affecting the slope of the sustained component, and blocked the fusion pore expansion. This suggests that Syt VII is the Ca2+ sensor of DCV fusion machinery and is an essential factor for the establishment and maintenance of the pool size of releasable DCVs in PC12 cells.

  5. Canine specific ELISA for coagulation factor VII

    DEFF Research Database (Denmark)

    Knudsen, Tom; Kjelgaard-Hansen, Mads; Tranholm, Mikael;

    2011-01-01

    available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable...

  6. Genetics Home Reference: mucopolysaccharidosis type VII

    Science.gov (United States)

    ... becomes cloudy, which can cause significant vision loss. People with MPS VII may also have recurrent ear infections and hearing loss. Affected individuals may have developmental delay and progressive intellectual disability, although intelligence is unaffected in some people with ...

  7. Extraction of Tc(VII) and Re(VII) on TRU resin

    Energy Technology Data Exchange (ETDEWEB)

    Guerin, Nicolas; Riopel, Remi; Kramer-Tremblay, Sheila [Canadian Nuclear Laboratories (CNL), Chalk River, ON (Canada). Radiobiology and Health Branch; De Silva, Nimal; Cornett, Jack [Ottawa Univ., ON (Canada). Dept. of Earth and Environmental Sciences; Dai, Xiongxin [China Institute for Radiation Protection, Beijing (China)

    2017-06-01

    TRU resin can be used to rapidly and selectively extract Tc(VII) and Re(VII). The retention capacity curves of Tc(VII) and Re(VII) for HNO{sub 3}, HCl, H{sub 2}SO{sub 4} and H{sub 3}PO{sub 4} solutions were studied and prepared. Tc(VII) and Re(VII) were simultaneously extracted in 2 M H{sub 2}SO{sub 4} and 1.5 M H{sub 3}PO{sub 4} and were effectively separated from Mo(VI) and Ru(III). Tc(VII) and Re(VII) remained strongly bonded to the resin even after washing using a large volume of 2 M H{sub 2}SO{sub 4} at a relatively high flow rate. Also, they were both completely eluted from the resin using 15 mL of near boiling water, an eluent directly compatible for ICP-MS instrument measurements.

  8. Establecimiento de un material de referencia de trabajo para interleucina-2 recombinante

    Directory of Open Access Journals (Sweden)

    Maribel Vega

    2005-08-01

    Full Text Available Resumen La interleucina-2 es una glicoproteína humana de 133 aminoácidos que resulta vital para obtener una respuesta inmunológica efectiva. En el Centro de Ingeniería Genética y Biotecnología ha podido obtenerse por vía recombinante a partir de una cepa de Escherichia coli. Para el control de la calidad de cada lote producido se hizo necesaria la elaboración de un material de referencia de trabajo. En esta publicación se describen los pasos seguidos en la obtención del lote candidato y la preparación del material de referencia como tal. Se demostró que el patrón de trabajo preparado es homogéneo para el uso en las técnicas analíticas de control de calidad de la interleucina-2 y se predijo una pérdida de actividad inferior al 5 % anual mediante un estudio de estabilidad acelerada. Se obtuvieron valores adecuados para la pureza del material de referencia, evaluada por electroforesis y para la actividad biológica, que se estableció con trazabilidad al material de referencia internacional para este producto.Establishment of a working reference material for recombinant interleukin-2 Interleukin-2 is a human glycoprotein with 133 aminoacids that is crucial for attaining an effective immunological reaction. In the Center of Genetic Engineering and Biotechnology this protein has been obtained as a recombinant product from Eschericia coli bacteria. For the quality control of every batch produced, it was necessary to establish a working reference material. In this paper we describe the steps taken to obtain a candidate batch and the preparation of the working material as such. It was proved that the working pattern obtained.is homogenous for its use in the analytical techniques of quality control of interleukin-2. A loss of activity lower than an annual 5 % was predicted by an accelerated stability study. Adequate values were attained for the purity of the reference material, evaluated by electrophoresis, and for the biological

  9. Estado actual de la vacuna recombinante contra el virus del papiloma humano

    Directory of Open Access Journals (Sweden)

    Wendy Chan Acón

    2008-12-01

    Full Text Available La infección por el virus del papiloma humano (VPH es una enfermedad de transmisión sexual común. Alrededor del mundo millones de personas están infectadas y el resto de la población en general tiene un riesgo de contraer la infección superior al 50%. El virus se asocia aproximadamente a un 100% de los casos de cáncer cervical; a un 100% de las neoplasias cervicales intraepiteliales grados 1, 2, 3; a un 40% de los casos de cáncer de vulva, vagina y pene, 100% de las verrugas genitales; a un 100% de las papilomatosis respiratorias recurrentes; a un 90% del cáncer anal y a un 12% del cáncer de cabeza y cuello, predominantemente en orofaringe y amígdala. Actualmente, el uso de dos vacunas está aprobado en diversos países: Gardasil® y Cervarix®. Ambas están compuestas por proteínas L1 de VPH, en forma de partículas no infecciosas similares al virus (VLPs producidas por tecnología de ADN recombinante, adsorbidas en adyuvantes que contienen aluminio. La eficacia hallada en diversos estudios en sujetos no expuestos previamente al virus se encuentran en el rango del 98.8% al 100.0% para la prevención de neoplasias cervicales, vulvares y vaginales intraepiteliales, grados 2 y 3, relacionados con el VPH-16/18, además de los adenocarcinomas in situ y verrugas genitales causadas por VPH- 16/18/6/11 en el caso de Gardasil® y una eficacia del 100% en el caso de Cervarix® para la prevención de neoplasias cervicales grado 2 y 3 relacionados con el VPH-16/18. La eficacia de ambas se mantiene alrededor de los 5 años. Hasta el momento no se le ha atribuido a la vacuna ningún efecto terapéutico, solo se administra con fines profilácticos, sin embargo, esta no debe ser considerada como un sustituto de las pruebas de tamizaje para la prevención del cáncer cervical.

  10. European Energy Law. Report VII

    Energy Technology Data Exchange (ETDEWEB)

    Hammer, U.; Roggenkamp, M.M. (eds.)

    2010-04-15

    The European Energy Law Report VII presents in four parts an overview of the most important legal developments in the field of EU and national energy and climate change law as discussed at the 2009 European Energy Law Seminar. The first part examines recent developments in EU climate change regulation. It includes an overview of the revision of the EU Directive governing CO2 emissions trading and a more detailed analysis of the new allocation regime, including the auctioning of emission rights. This is followed by an analysis of the competitiveness of the new EU emissions trading Directive and recent developments in the US regarding emissions trading. The second part focuses on the energy liberalisation process and the Third Energy Package. Following an overview of recent developments in EU competition law and the impact of the Sector Inquiry on the energy sector, this part concentrates particularly on the new Internal Energy Market Directives and most particularly on the legality of the unbundling provisions. Subsequently, the focus is on the role of the TSOs. After an analysis of the need for an increased cooperation of the German TSOs, another chapter discusses the need for more consolidation in the European gas transmission market and the role of regulation therein. Is there a new role for nuclear energy? In order to secure long term energy supply, the focus turns again to nuclear energy. The third part of this report discusses two elements of nuclear liability. First, it analyses the issue of the possible privatisation of the nuclear sector and its impact on long term liability. Although the focus is on the UK nuclear sector, a comparison is made with some other regulatory regimes. Next, the role of nuclear energy is discussed as an instrument to reduce CO2 emissions and, more particularly as a means to reach the 20-20-20 targets. The question is raised whether the international rules on civil liability provide sufficient protection to victims of nuclear

  11. ESTUDO DE METODOLOGIAS PARA AVALIAÇÃO DO HORMÔNIO DA PARATIREÓIDE HUMANO RECOMBINANTE

    OpenAIRE

    Fernanda Pavani Stamm

    2013-01-01

    O hormônio da paratireóide humano (hPTH) é um polipeptídio constituído de 84 aminoácidos e tem função essencial como regulador da homeostase dos íons cálcio e fosfato, e manutenção da estrutura óssea. O hormônio da paratireóide humano recombinante, rhPTH (1-34), é produzido pela tecnologia do DNA em Escherichia coli, apresenta a sequência de aminoácidos responsável pela porção biologicamente ativa do paratormônio natural e é usado clinicamente para o tratamento da osteoporose d...

  12. Eritropoyetina recombinante humana en pacientes con enfermedad renal crónica en etapa de prediálisis

    OpenAIRE

    María del Carmen Popoca Martínez; Iván Henríquez Moreu; Antonio Méndez Durán; Jorge Revilla Beltri; Aarón Molina Pérez

    2008-01-01

    Objetivo: evaluar el efecto de la eritropoyetina recombinante humana en pacientes con anemia y enfermedad renal crónica, en etapa de prediálisis, y su repercusión en la calidad de vida. Material y métodos: se seleccionaron 20 pacientes con enfermedad renal crónica en etapa de prediálisis, a quienes se realizó biometría hemática completa y química sanguínea en cada visita médica; la escala de calidad de vida se aplicó en la visita inicial y final del tratamiento. se administraron 50 Ul/g de er...

  13. INMUNOGENICIDAD DE LA PROTEÍNA RECOMBINANTE ASP1R DE Ancylostoma caninum EN UN MODELO MURINO

    Directory of Open Access Journals (Sweden)

    Maria Giraldo G

    2009-08-01

    Full Text Available Objetivo. Construir un plásmido recombinante que exprese la proteína ASP1r de Ancylostoma caninum y evaluar su capacidad inmunogénica en un modelo murino. Materiales y métodos. Se realizó extracción de ARN de parásitos adultos de Ancylostoma caninum, se amplificó por RT –PCR el gen de la proteína ASP1. Este gen fue insertado en el vector pcDNA3. El inserto fue digerido con Bamh1 y EcoR1 y clonado direccionalmente. Posteriormente, se llevó a cabo transformación y selección de las células de E. coli DH5a competentes con el producto de la ligación. Se realizó un tamizaje por PCR confirmando la presencia del gen ASP1. El vector pcDNA3-ASP1 fue administrado vía intraglandular en la parotida e intramuscular en ratones Balb/c. En estos animales se les realizó determinación de anticuerpos en suero y saliva mediante las técnicas de ELISA e inmunohistoquímica. Resultados. Se determinó que el plásmido pcDNA3-ASP1 fue incorporado y expresado células E. coli DH5a. Este plásmido recombinante indujo la producción de anticuerpos Anti-ASP1 específicos en ratones Balb/c. Conclusiones. Se logró demostrar que la utilización de pcDNA3-ASP1 no produjo reacciones desfavorables en ratones Balb/c, además indujo respuesta humoral contra la proteína pcDNA3-ASP1 de excreción/secreción de Ancylostoma caninum en ratones.

  14. Vectores recombinantes basados en el virus modificado de ankara (MVA) como vacunas preventivas y terapéuticas contra el SIDA

    OpenAIRE

    Jonathan L Heeney; Mooij, Petra; Nájera García, José Luis; Jiménez, Victoria; Esteban, Mariano; Gómez, Carmen E.

    2005-01-01

    Vectores Recombinantes basados en el Virus Modificado de Ankara (MVA) como Vacunas Preventivas y Terapéuticas contra el SIDA. Losvirus recombinantes de la invención contienen secuencias que se encuentran insertadas en el mismo sitio de inserción del MVA y permiten la expresión simultánea de varios antígenos, una proteína Env del VIH-I consistente en una proteína gpl20 carente de secuencias correspondientes a la proteína gp41, y una proteína quiméricade fusión de Gag, Pol y Nef. Son virus esta...

  15. Vectores recombinantes basados en el virus modificado de Ankara (MVA) com vacunas preventivas y terapeúticas contra la hepatitis C

    OpenAIRE

    Esteban, Mariano; Gómez, Carmen E.; Perdiguero, Beatriz

    2014-01-01

    [ES] Los virus recombinantes de la invención contienen secuencias que se encuentran insertadas en el mismo sitio de inserción del MVA y que permiten la expresión simultáneamente de varios antígenos del VHC, concretamente las proteínas maduras estructurales (Core, E1, E2 y p7) y no estructurales (NS2, NS3, NS4A, NS4B, NS5A más los 201 aminoácidos de la región N-terminal de NS5B). Con ello se consiguen virus recombinantes estables, que permiten el desencadenamiento de una respuesta inmune contr...

  16. Toxicidad de δ-endotoxinas recombinantes de Bacillus thuringiensis sobre larvas de la polilla guatemalteca (Tecia solanivora (Lepidóptera: Gelechiidae

    Directory of Open Access Journals (Sweden)

    Javier Hernández-Fernández

    2009-01-01

    Full Text Available Con el objetivo de determinar la actividad tóxica específica de las proteínas recombinantes Cry1Aa, Cry1Ac, Cry1B y Cry1C de Bacillus thuringiensis (Bt, sobre larvas de primer instar de Tecia solanivora se estableció la CL50 para las toxinas. Para este fin se implementó la cría masiva de este insecto bajo condiciones de laboratorio, 58±5% de humedad relativa, 18±5ºC de temperatura y un fotoperiodo de 23 h oscuridad y 1 h luz. Se utilizó una dieta seminatural consistente en láminas de papa variedad parda pastusa autoclavada con solución preservante (ácido ascórbico 7 g/L y metilparabeno 5 g/L, ya que fue estable en el tiempo, garantizó la reproducibilidad de los resultados y fue de fácil evaluación. Las proteínas recombinantes se evaluaron a una concentración de 0,1 μg/cm2. Los resultados obtenidos de porcentaje de mortalidad indicaron que no había diferencias entre las delta-endotoxinas recombinantes Cry1Aa, Cry1Ac, Cry1B y Cry1C de Bt (PPalabras clave: Bacillus thuringiensis; proteínas recombinantes; Tecia solanivora; papa parda pastusa; Bacillus thuringiensis; bioassay; recombinant endotoxin; Tecia solanivora; pastusa potato variety.

  17. Synthesis of a novel organic-inorganic hybrid of polyaniline/titanium phosphate for Re(VII) removal.

    Science.gov (United States)

    Gao, Yang; Chen, Changlun; Chen, He; Zhang, Rui; Wang, Xiangke

    2015-05-21

    The organic-inorganic hybrid material of polyaniline/titanium(IV) (PANI/Ti(HPO4)2) was synthesized by an oxidative polymerization reaction. The PANI/Ti(HPO4)2 was applied to remove Re(VII). The size of Ti(HPO4)2 nanoplates has no obvious effect on the sorption capacity. The effects of various environmental factors (such as pH, extra anions (NO3(-) and MO4(2-)) and temperature) on Re(VII) sorption to PANI/Ti(HPO4)2 were investigated by batch experiments. The sorption kinetics followed a pseudo-second-order model. The nitrogen-containing functional groups of PANI promoted Re(VII) sorption. The PANI/Ti(HPO4)2 exhibited excellent maximum sorption capacity to Re(VII) (47.62 mg g(-1)), which was superior to that of PANI (10.75 mg g(-1)) and much higher than that of many other sorbents. The sorption isotherms of Re(VII) can be well fitted with the Langmuir model. Re(VII) sorption decreased with increasing solution pH at pH > 4.0, which implied that Re(VII) sorption on PANI/Ti(HPO4)2 might be attributed to the outer-sphere complexation between amine and imine groups on the surface of PANI/Ti(HPO4)2 and Re(VII). This study implies that the hybrid material of PANI/Ti(HPO4)2 can be regarded as a potential sorbent to remove Re(VII) and its analogues from large volumes of aqueous solutions.

  18. Clonamiento, expresión y seroreactividad del antígeno recombinante flagelina de Bartonella bacilliformis

    Directory of Open Access Journals (Sweden)

    Karen Gallegos V

    2005-03-01

    Full Text Available Objetivos. Clonar el gen de la flagelina A (flaA de Bartonella bacilliformis, expresar y evaluar preliminarmente la seroreactividad de la proteína recombinante a sueros de pacientes con Bartonelosis por B. bacilliformis. Materiales y Métodos. Se diseñó una pareja de oligonucleótidos iniciadores -BbFlaA1 y BbFlaA2- para la amplificación del gen completo de la flagelina flaA de B. bacilliformis. El producto de amplificación obtenido se clonó en pGEM y luego se subclonó en el vector de expresión pGEX4T-1. Se indujo la expresión de la proteína de fusión rBbFlaA-GST con isopropil tio-β -D-galactosido (IPTG. La proteνna de fusiσn producida fue digerida con trombina para liberarla de GST. Finalmente, una prueba de ELISA fue estandarizada para detectar los anticuerpos IgG contra la proteína de fusión rBbFlaA-GST y rBbflaA libre de GST. Se evaluaron sueros de pacientes con diagnóstico de Bartonelosis por B. Bacilliformis (n= 30, sueros de individuos sanos (n= 20 y sueros de pacientes con otras enfermedades de posible reactividad cruzada; entre ellas, Brucelosis (n= 3, leptospirosis (n= 3 y salmonelosis (n=7. Resultados. Se determinó que para la expresión óptima en E. coli BL21 de la proteína de fusión rBbFlaA se requiere que el cultivo crezca en caldo LB/ampicilina a 30 °C suplementado con 2% de glucosa a partir de un preinóculo de 100 µL (crecido por toda la noche, hasta que alcance una densidad óptica de 1 OD600 y se induzca por dos horas con 2,5 mM de IPTG. Finalmente, el 57,6 % (17 de 30 sueros de pacientes con diagnóstico confirmado de bartonelosis reaccionaron con la proteína recombinante BbFlaA en el formato de ELISA. Conclusiones. Se logró expresar exitosamente en E. coli la proteína recombinante BbFlaA de B. bacilliformis, determinándose un protocolo de expresión y de purificación de rBbFlaA para la producción de esta proteína. Así también, el antígeno rBbFlaA es reconocido por anticuerpos de sueros de

  19. Intelsat VII communications capabilities and performance

    Science.gov (United States)

    Abdel-Nabi, T.; Koh, E.; Kennedy, D.

    This paper describes the general characteristics of the Intelsat VII communications payload and analyzes the suitability of its design for digital transmission, for efficient incorporation of small earth stations into the Intelsat network, and for a relatively straightforward transition from the current Intelsat VA/VI configuration. An overview is presented of the comunications performance of the Intelsat VII satellite for the more important Intelsat digital and analog services, and earth stations standards. Specifically, the advantages, in terms of performance and capabilities, of the improved transponder linearity characteristics associated with the C-Band SSPA's and the K(u)-Band linearized TWTA's are addressed. The enhanced ability of this spacecraft to provide bandwidth where required from several possible orbital locations is discussed.

  20. Producción de la proteína recombinante JAK2 de ratón

    Directory of Open Access Journals (Sweden)

    Ruth E. Garzón

    2000-03-01

    Full Text Available El presente trabajo describe la producción de la proteina recombinante JAK2, empleando un sistema de vectores de expresión baculovirales. La infección de células Sf9, derivadas del tejido de ovario de Spodoptera frugiperda, permitió establecer las condiciones óptimas de dilución del virus en 10 pllml de medio y de tiempo de recolección en 72 horas postinfección. Se recolectaron y ultrafiltraron extractos correspondientes a la infección de 5 x 10' células, aproximadamente, y se sometieron a cromatografia de intercambio iónico (DEAE-Sephacel, lográndose purificar parcialmente la proteina JAK2 por medio de un gradiente continuo de fuerza iónica (NaCI 10-500 mM. La presencia de JAK2 se verificó por Western blot, empleando un anticuerpo policlonal producido en conejo contra los residuos 758-776 de JAK2 de ratón. Los resultados indicaron que la proteína obtenida por este sistema de expresión se sintetiza en forma fosforiladalactivada.

  1. Expresión y sororreactividad de la lipoproteína recombinante de 43-kda de Bartonella bacilliformis

    Directory of Open Access Journals (Sweden)

    Carlos Padilla R

    2006-07-01

    Full Text Available Objetivo: La lipoproteína de 43-kDa de Bartonella bacilliformis fue obtenida en su forma recombinante (rBbLppB y purificada para evaluar su serorreactividad mediante ELISA. Materiales y métodos: Los niveles de anticuerpos IgG e IgM humanos en los sueros de pacientes con Bartonelosis confirmada y sueros de otras enfermedades (salmonelosis, Brucelosis y leptospirosis frente a rBbLppB fueron evaluados por ELISA, se utilizó sueros de personas sanas como controles. Resultados: La sensibilidad y la especificidad del ELISA IgG fueron 70,4% y 90% respectivamente. Asimismo, la sensibilidad y especificidad de ELISA IgM fueron 85,2% y 90% respectivamente. Conclusiones: Estos resultados demuestran que el ELISA usando rBbLppB tiene una buena sensibilidad y especificidad y puede ser considerada como un buen antígeno para el diagnóstico de Bartonelosis causada por B. bacilliformis.

  2. Association of factor VII protein concentration with lifestyle factors

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Møller, L; Jespersen, J

    1998-01-01

    -sectional study examined the association between several behavioural variables (body mass index, physical activity, tobacco or alcohol consumption) or physiological variables (total cholesterol, HDL-cholesterol, triglycerides, glucose, insulin, fibrinogen, resting pulse, systolic blood pressure, bleeding time......) and FVII:Ag in 439 51-y-old Danish men. In the multivariate analyses, body mass index (BMI), low physical activity, total cholesterol, short bleeding time, and insulin showed an independent positive association with FVII:Ag. The strongest independent association with FVII:Ag was found for total cholesterol...

  3. Proteínas recombinantes útiles para el diagnóstico, prevención y estudios patogénicos de la leptospirosis : Estudio de mecanismos patogénicos involucrados

    OpenAIRE

    Prêtre, Gabriela

    2011-01-01

    Objetivo general El propósito general de este trabajo es: 1. Generar herramientas moleculares que sean útiles contra la leptospirosis, ya sea para su diagnóstico, prevención o un mejor conocimiento de su patogénesis. 2. Estudiar mecanismos patogénicos involucrados en la leptospirosis. Objetivos específicos 1. Clonado, expresión y purificación de proteínas recombinantes. 2. Producción de antisuero contra las proteínas recombinantes....

  4. Investigating the air oxidation of V(II) ions in a vanadium redox flow battery

    Science.gov (United States)

    Ngamsai, Kittima; Arpornwichanop, Amornchai

    2015-11-01

    The air oxidation of vanadium (V(II)) ions in a negative electrolyte reservoir is a major side reaction in a vanadium redox flow battery (VRB), which leads to electrolyte imbalance and self-discharge of the system during long-term operation. In this study, an 80% charged negative electrolyte solution is employed to investigate the mechanism and influential factors of the reaction in a negative-electrolyte reservoir. The results show that the air oxidation of V(II) ions occurs at the air-electrolyte solution interface area and leads to a concentration gradient of vanadium ions in the electrolyte solution and to the diffusion of V(II) and V(III) ions. The effect of the ratio of the electrolyte volume to the air-electrolyte solution interface area and the concentrations of vanadium and sulfuric acid in an electrolyte solution is investigated. A higher ratio of electrolyte volume to the air-electrolyte solution interface area results in a slower oxidation reaction rate. The high concentrations of vanadium and sulfuric acid solution also retard the air oxidation of V(II) ions. This information can be utilized to design an appropriate electrolyte reservoir for the VRB system and to prepare suitable ingredients for the electrolyte solution.

  5. Pesquisa de IgA contra o antígeno recombinante HspX de Mycobacterium tuberculosis no diagnóstico de tuberculose pleural

    OpenAIRE

    Limongi,Loanda Carvalho Sant' Ana; Olival,Liliane; Conde, Marcus Barreto; Junqueira-Kipnis, Ana Paula

    2011-01-01

    OBJETIVO: Avaliar a acurácia da dosagem de IgA contra o antígeno recombinante HspX no líquido pleural e no soro de pacientes com derrame pleural para o diagnóstico de tuberculose pleural. MÉTODOS: Estudo transversal de teste diagnóstico. Amostras de líquido pleural e de soro de pacientes com derrame pleural e suspeita de tuberculose pleural foram avaliadas para a determinação da densidade óptica de IgA contra HspX utilizando ELISA indireto. RESULTADOS: Foram avaliadas amostras de líquido pleu...

  6. Alternativa terapeutica en la isquemia cerebral empleando la Eritropoyetina humana recombinante (rHu-EPO CIM como neuroprotector.

    Directory of Open Access Journals (Sweden)

    Iliana Sosa Testé

    2005-01-01

    Full Text Available Las enfermedades vasculares constituyen las terceras causas de muerte en Cuba. Su variante isquémica es causa de invalidez y perdida de la calidad de la vida. La búsqueda de agentes neuroprotectores es muy importante sí tenemos en cuenta que nuestra población está envejecida. Muchos son las razones por la que una molécula con propiedades neuroprotectoras en los modelos animales no tenga los resultados en ensayos clínicos en humanos. Este trabajo analiza y discute como mejorar estos aspectos. La Eritropoyetina humana recombinante (rHu EPO, conocido por su papel en la diferenciación del eritrocito, ha mostrado ser neuroprotector durante la isquemia cerebral en los modelos de animales adultos. De esta molécula se exponen los mecanismos de acción, que son las bases de los resultados positivos alcanzaron en las investigaciones preclínicas y los ensayos clínicos, que lo proponen como el neuroprotector ideal. Finalmente se comparan los resultados alcanzados por los autores en las investigaciones con la rHu EPO producida por el Centro Molecular de Inmunología de Cuba (CIM. El efecto de la aplicación de rHu EPO se evidenció por la disminución del edema cerebral, preservación de la conducta exploratoria y la disminución de la incidencia de señales clínicas de infarto cerebral y alteraciones patológicas. El grupo de resultados demostró las diferencias significantes entre los animales controles y tratados con el rHu EPO con los no tratados que sugieren que el rHu EPO debe ser considerado como una alternativa terapéutica contra el infarto cerebral en su fase aguda.

  7. 40 CFR Appendixes I-Vii to Part 85 - [Reserved

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false I Appendixes I-VII to Part 85 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF AIR POLLUTION FROM MOBILE SOURCES Appendixes I-VII to Part 85...

  8. Physics and engineering design for Wendelstein VII-X

    Energy Technology Data Exchange (ETDEWEB)

    Beidler, C.; Grieger, G.; Herrnegger, F.; Harmeyer, E.; Kisslinger, J.; Lotz, W.; Maassberg, H.; Merkel, P.; Nuhrenberg, J.; Rau, F.; Sapper, J.; Sardei, F.; Scardovell, R.; Schluter, A.; Woblig, H. (Max-Planck-Institut fuer Plasmaphysik, Garching (Germany, F.R.))

    1990-01-01

    This paper reports on the development of the future experiment Wendelstein VII-X. A helical advanced stellarator configuration has been chosen. The goals of W VII-X are to continue the development of the modular stellarator, to demonstrate the reactor capability of this stellarator line, and to achieve quasi-steady-state operation in a temperature regime > 5 keV.

  9. Re(VII) and Tc(VII) trioxo complexes stabilized by tridentate ligands and their potential use as radiopharmaceuticals

    NARCIS (Netherlands)

    Hahn, Eva M.; Casini, Angela; Kuehn, Fritz E.

    2014-01-01

    Tc(VII) and Re(VII) trioxo complexes are currently arousing interest because of their potential use as radiopharmaceuticals due to their hydrophilic character and stability in a biological environment. The radioactive isotopes Tc-99m and Re-188 are readily obtained from commercially available Mo-99/

  10. Aplicación del análisis de riesgo a la producción de proteínas recombinantes expresadas en Escherichia coli

    Directory of Open Access Journals (Sweden)

    José García

    2012-04-01

    Full Text Available En este trabajo se aplicó el análisis de riesgo, empleando la metodología de análisis de modos y efectos de fallas a los procesos de fermentación que utilizan la bacteria Esherichia coli como hospedero, para obtener proteínas recombinantes con fines terapéuticos, vacunales o diagnósticos. Se realizó el análisis del tipo y probabilidad de ocurrencia de las fallas en el proceso fermentativo, la evaluación del impacto en la calidad del mismo y la probabilidad de detección de dichas fallas. Se evaluó la severidad, probabilidad de ocurrencia y probabilidad de detección y se calculó el número de probabilidades de riesgo. Además, se emplearon técnicas utilizadas para el aseguramiento de la calidad como: tormenta de ideas y diagrama causa-efecto. Se concluye que las causas potenciales que tienen mayor influencia en las fallas de un proceso fermentativo de E. coli recombinante son: la inadecuada manipulación durante la inoculación, la presencia de fagos y el personal no calificado. Se proponen acciones a tomar para minimizar el riesgo.

  11. DESARROLLO DE UN POXVIRUS RECOMBINANTE QUE EXPRESA LA GLICOPROTEÍNA D DEL HERPESVIRUS BOVINO-1 Development of a Recombinant Poxvirus Expressing Bovine Herpesvirus-1 Glycoprotein D

    Directory of Open Access Journals (Sweden)

    JULIÁN RUIZ SÁENZ

    2012-12-01

    Full Text Available El herpesvirus bovino-1 (BHV-1 es un virus de genoma DNA perteneciente a la familia Herpesviridae, el cual afecta al bovino en el que provoca un amplio espectro de manifestaciones clínicas y pérdidas económicas. El principal componente inmunogénico de su envoltura es la glicoproteína D (gD, la cual ha sido caracterizada y utilizada como inmunógeno en distintos sistemas de expresión. El objetivo de este trabajo fue generar un poxvirus recombinante (Raccoonpox [RCN] que expresara una versión truncada de la gD del BHV-1 para ser usado como inmunógeno. Para ello, se amplificó el gen que codifica para la versión truncada de la gD, la cual se clonó en el plásmido de transferencia pTK/ IRES/tpa que posee sitios de homología a la timidinakinasa del poxvirus, un sitio interno de entrada al ribosoma (IRES y una señal secretoria (tPA, generando el constructo pTK/gD/IRES/tpa. Para generar el RCN recombinante, se tomaron células BSC-1, se infectaron con una cepa Silvestre del RCN (CDC/V71-I-85A a un índice de multiplicidad de infección de 0,05 y se transfectaron con el constructo pTK/gD/IRES/tpa; generándose diferentes poblaciones virales con y sin el gen de interés. Para seleccionar los virus recombinantes que expresaban el gen de interés, se realizó una selección de recombinantes negativos para timidina kinasa y positivos para la gD por tres rondas de purificación de placas en monocapas de células RAT-2 las cuales son mutantes para timidina kinasa y en presencia de bromodeoxiuridina. Los virus recombinantes se confirmaron por PCR y secuenciación de nucleótidos y se denominaron RCN-gD.Bovine Herpesvirus-1 is a DNA virus belonging to the family Herpesviridae, which affects cattle, causing a wide spectrum of clinical manifestations and economic losses. The main immunogenic component is its envelope glycoprotein D (gD, which has been characterized and used as immunogen in different expression systems. The aim of this work was to

  12. Respuesta de IFN-y e Il-4 en ratones inoculados con una proteína G recombinante del virus de la rabia

    Directory of Open Access Journals (Sweden)

    Edith Rojas-Anaya

    2015-01-01

    Full Text Available El objetivo de este estudio fue comparar el comportamiento de IFN-  e IL-4 en ratones inoculados con proteína G recombinante del virus de la rabia, que se expresa en maíz transgénico o baculovirus. Para este pr opósito, grupos de ratones se inocularon de la siguiente manera: Grupo 1, vacuna contra el virus de la rabia inactivado vía im; Grupo 2, proteína G recombinante deri vada de plantas por vía oral; Grupo 3, proteína G expresada en baculovirus vía im; Grupo 4, proteína G expresada en baculovirus por vía oral; Grupo 5, maíz no tran sformado por vía oral. Los niveles de IFN-  e IL-4 y los anticuerpos específicos se evaluaron cada 15 días. El desafío se realizó a los 60 días post inoculación (pi. Los grupos 1 y 3 promovieron un a mejor respuesta humoral. Por otro lado, los resultados demostraron que los mismos grupos mostraron los mejores niveles de IFN -  en el día 10 pi; mientras que la IL-4 se detectó en el día 15 pi. Para el estudio de supervivencia, el 83 % de los ratones inmunizados con vacuna inactivada, maíz y los inoculados im con extractos de baculovirus sob revivieron la infección viral. La proteína G de la rabia recombinante expresada en baculovirus promovió IFN-  y los anticuerpos de una manera similar a la vacuna de rabia inactivada. A pesar de esto los ratones alime ntados con maíz transgénico sobrevivieron al desafío en el mismo porcentaje que el grupo 3.

  13. Seguridad de la terapia de interferón alfa 2b recombinante más ribavirina en la hepatitis crónica C

    Directory of Open Access Journals (Sweden)

    Yoan Antonio Sánchez Rodríguez

    2011-03-01

    Full Text Available INTRODUCCIÓN: la hepatitis crónica C ha adquirido rango de pandemia. El virus de la hepatitis C se ha convertido en la causa principal de hepatitis crónica, cirrosis hepática, hepatocarcinoma, y trasplante de hígado a nivel mundial. OBJETIVO: identificar los efectos adversos asociados a la terapia combinada interferón alfa 2b recombinante más ribavirina durante la evolución del tratamiento y hasta 8 semanas después de finalizado, así como los principales efectos asociados a salidas temporales o definitivas de esta terapia. MÉTODOS: estudio de farmacovigilancia cuya serie estuvo conformada por 122 pacientes con hepatitis crónica C atendidos en el Instituto de Gastroenterología desde mayo de 2001 hasta mayo de 2006. Se utilizó interferón alfa 2b recombinante (3 millones de unidades 3 veces por semana más ribavirina (1 000 o 1 200 mg diarios en dependencia del peso corporal durante 48 semanas. RESULTADOS: el 88,5 % del total de casos presentó efectos adversos; de ellos el 79,5 % correspondió al síndrome seudogripal, seguido de manifestaciones hematológicas, neuropsiquiátricas, gastrointestinales, entre otras menos frecuentes. El 6,6 % de la serie presentó salidas temporales del tratamiento por efecto adverso distinto de la anemia y 4 pacientes, salidas definitivas del estudio, tres por anemia hemolítica severa y uno con hipertiroidismo no controlable. CONCLUSIONES: la terapia combinada interferón alfa 2b recombinante más ribavirina resulta segura, donde el mayor número de casos presentó síndrome seudogripal como efecto adverso más frecuente. Las manifestaciones hematológicas asociadas a las salidas definitivas del estudio permitieron recomendar seguimiento estricto de la hemoglobina y profundizar en el diagnóstico y tratamiento de los principales efectos adversos presentes en otros sistemas y asociados a esta terapia.

  14. TEH võrgustik - viis aastat Eestis / Marika Kusnets

    Index Scriptorium Estoniae

    Kusnets, Marika

    2005-01-01

    Eestis on tervist edendavate haiglate (TEH) võrgustik tegutsenud juba viis aastat, sellega on liitunud 22 haiglat. Tänavust konverentsi peeti teemal "Tervise edendamine ja kvaliteedi tagamine haiglas: standardid ja praktika"

  15. TEH võrgustik - viis aastat Eestis / Marika Kusnets

    Index Scriptorium Estoniae

    Kusnets, Marika

    2005-01-01

    Eestis on tervist edendavate haiglate (TEH) võrgustik tegutsenud juba viis aastat, sellega on liitunud 22 haiglat. Tänavust konverentsi peeti teemal "Tervise edendamine ja kvaliteedi tagamine haiglas: standardid ja praktika"

  16. Settling Title VII Disputes: A Role for the Arbitrator

    Science.gov (United States)

    Owens, Stephen D.

    1976-01-01

    Selected arbitration cases illustrate the dilemma facing the arbitrator when employment practices that violate both the collective bargaining agreement and Title VII must be resolved. Problems in dealing with race and sex discrimination are discussed. (LBH)

  17. Meenutusi VII Riigikogu päevilt / Helle Ruusing

    Index Scriptorium Estoniae

    Ruusing, Helle, 1966-

    2003-01-01

    Katkendeid Riigikogu Kantselei 2002. aastal gümnaasiumiõpilastele korraldatud VII Riigikogu liikmete mälestuste kogumise võistlusele laekunud töödest. Eesti taasiseseisvumisaja esimese parlamendi, VII Riigikogu töökorraldust, töötingimusi Toompea lossis, tööd fraktsioonides ja komisjonides meenutavad Juhan Aare, Avo Kiir, Kalle Jürgenson, Kalev Raave, Ants Käärma, Paul-Olev Mõtsküla, Tiit Sinissaar, Peeter Lorents

  18. Análise de innterferon humano recombinante presente em formulações farmacêuticas por espectrometria de massa

    OpenAIRE

    Andrade, Sinéa Mendes de

    2010-01-01

    Os interferons (INFs) são proteínas produzidas por células animais em resposta a infecções virais. Essas substâncias possuem vários efeitos farmacológicos, incluindo antiviral, antitumoral e imunomodulatório. Com o advento da tecnologia do DNA recombinante, vários interferons passaram a ser produzidos e comercializados como Biofármacos. O mais importante é o α2b, que é uma das bases do tratamento das hepatites virais. A única norma oficial que regulamenta a produção de INF-α2b é a Farmacopéia...

  19. Relato do primeiro paciente brasileiro com a forma infantil da doença de Pompe tratado com alfa-glicosidase recombinante humana

    OpenAIRE

    2008-01-01

    Objetivo: Relatar o primeiro caso de forma infantil da doença de Pompe tratado no Brasil. Descrição: Trata-se de doença de depósito lisossomal que se caracteriza por defeitos da enzima alfa-glicosidase ácida, com acúmulo intracelular de glicogênio, principalmente nos músculos. São descritas a forma infantil e tardia. Desde 2006, está disponível tratamento com enzima recombinante humana. Descreve-se o primeiro caso de forma infantil da doença tratado no Brasil. Trata-se de menina com 2,5 meses...

  20. Obtención de anticuerpos monoclonales de ratón contra proteasa de cisteína 5 recombinante de Entamoeba histolytica

    Directory of Open Access Journals (Sweden)

    Juanita Trejos S.

    2012-08-01

    Full Text Available Objetivo. Obtener anticuerpos monoclonales de ratón contra la proteasas de cisteína 5 (EhCP5 de Entamoeba histolytica. Materiales y métodos. Se inmunizaron ratones BALB/c por vía intraperitoneal con adyuvante de Freund completo e incompleto con la proteína recombinante EhCP5 obtenida a partir del cultivo de E.coli DH5α trasfectada con el vector recombinante pJC45 que expresa dicha proteína. Se seleccionó el animal con mejor respuesta de anticuerpos. Al cual se le extrajo su bazo como fuente de linfocitos B, los cuales se fusionaron utilizando PEG con células de mieloma de ratón SP2-0/Ag14. Se procedió a selección de los hibridomas y a la evaluación de los sobrenadantes de las colonias que crecieron a los 7 días mediante ELISA. Los hibridomas con valores más altos de anticuerpos específicos contra la proteína EhCP5r se seleccionaron, y los clones obtenidos por diluciones limitantes fueron expandidos. Resultados. A partir de un clon secretor estable se purifico el anticuerpo monoclonal anti EhCP5r del isotipo IgG1 por cromatografía de afinidad con proteína G. Los clones fueron expandidos in vivo e in vitro. Con el anticuerpo purificado se diseñaron tres sistemas de captura para evaluar la aplicabilidad del anticuerpo monoclonal anti EhCP5r como método inmunodiagnóstico. Conclusiones. Se logro la producción de un anticuerpo monoclonal específico contra EhCP5r que permite diferenciar Entamoeba histolytica de Entamoeba dispar.

  1. Experimental evidence for competitive growth advantage of genotype VII over VI: implications for foot-and-mouth disease virus serotype A genotype turnover in nature.

    Science.gov (United States)

    Mohapatra, J K; Subramaniam, S; Singh, N K; Sanyal, A; Pattnaik, B

    2012-04-01

    In India, systematic genotype replacement has been observed for serotype A foot-and-mouth disease virus. After a decade of co-circulation of genotypes VI and VII, genotype VII emerged as the single dominant genotype since 2001. To derive possible explanations for such epochal evolution dynamics, in vitro intergenotype growth competition experiments involving both co- and superinfection regimes were conducted. Coinfection of BHK-21 cells demonstrated abrupt loss in the genotype VI viral load with commensurate increase in the load of genotype VII as measured by the genotype differentiating ELISA, RT-PCR and real-time RT-PCR. The superinfection dynamics was shaped by temporal spacing of infection, where the invading genotype VII took more number of passages than coinfection to eventually overtake the resident genotype VI. It was speculated that such superior replicative fitness of genotype VII could have been a possible factor for the ultimate dominance of genotype VII in nature. Copyright © 2011 Elsevier Ltd. All rights reserved.

  2. Estado actual de la vacuna recombinante contra el virus del papiloma humano State of the art on human papillomavirus vaccine

    Directory of Open Access Journals (Sweden)

    Wendy Chan Acón

    2008-12-01

    Full Text Available La infección por el virus del papiloma humano (VPH es una enfermedad de transmisión sexual común. Alrededor del mundo millones de personas están infectadas y el resto de la población en general tiene un riesgo de contraer la infección superior al 50%. El virus se asocia aproximadamente a un 100% de los casos de cáncer cervical; a un 100% de las neoplasias cervicales intraepiteliales grados 1, 2, 3; a un 40% de los casos de cáncer de vulva, vagina y pene, 100% de las verrugas genitales; a un 100% de las papilomatosis respiratorias recurrentes; a un 90% del cáncer anal y a un 12% del cáncer de cabeza y cuello, predominantemente en orofaringe y amígdala. Actualmente, el uso de dos vacunas está aprobado en diversos países: Gardasil® y Cervarix®. Ambas están compuestas por proteínas L1 de VPH, en forma de partículas no infecciosas similares al virus (VLPs producidas por tecnología de ADN recombinante, adsorbidas en adyuvantes que contienen aluminio. La eficacia hallada en diversos estudios en sujetos no expuestos previamente al virus se encuentran en el rango del 98.8% al 100.0% para la prevención de neoplasias cervicales, vulvares y vaginales intraepiteliales, grados 2 y 3, relacionados con el VPH-16/18, además de los adenocarcinomas in situ y verrugas genitales causadas por VPH- 16/18/6/11 en el caso de Gardasil® y una eficacia del 100% en el caso de Cervarix® para la prevención de neoplasias cervicales grado 2 y 3 relacionados con el VPH-16/18. La eficacia de ambas se mantiene alrededor de los 5 años. Hasta el momento no se le ha atribuido a la vacuna ningún efecto terapéutico, solo se administra con fines profilácticos, sin embargo, esta no debe ser considerada como un sustituto de las pruebas de tamizaje para la prevención del cáncer cervical.Genital human papillomavirus (HPV is one of the most common sexually transmitted infections worldwide. Millions of persons are now infected and the lifetime risk of HPV

  3. Benchmarking ENDF/B-VII.0

    Science.gov (United States)

    van der Marck, Steven C.

    2006-12-01

    The new major release VII.0 of the ENDF/B nuclear data library has been tested extensively using benchmark calculations. These were based upon MCNP-4C3 continuous-energy Monte Carlo neutronics simulations, together with nuclear data processed using the code NJOY. Three types of benchmarks were used, viz., criticality safety benchmarks, (fusion) shielding benchmarks, and reference systems for which the effective delayed neutron fraction is reported. For criticality safety, more than 700 benchmarks from the International Handbook of Criticality Safety Benchmark Experiments were used. Benchmarks from all categories were used, ranging from low-enriched uranium, compound fuel, thermal spectrum ones (LEU-COMP-THERM), to mixed uranium-plutonium, metallic fuel, fast spectrum ones (MIX-MET-FAST). For fusion shielding many benchmarks were based on IAEA specifications for the Oktavian experiments (for Al, Co, Cr, Cu, LiF, Mn, Mo, Si, Ti, W, Zr), Fusion Neutronics Source in Japan (for Be, C, N, O, Fe, Pb), and Pulsed Sphere experiments at Lawrence Livermore National Laboratory (for 6Li, 7Li, Be, C, N, O, Mg, Al, Ti, Fe, Pb, D 2O, H 2O, concrete, polyethylene and teflon). For testing delayed neutron data more than thirty measurements in widely varying systems were used. Among these were measurements in the Tank Critical Assembly (TCA in Japan) and IPEN/MB-01 (Brazil), both with a thermal spectrum, and two cores in Masurca (France) and three cores in the Fast Critical Assembly (FCA, Japan), all with fast spectra. In criticality safety, many benchmarks were chosen from the category with a thermal spectrum, low-enriched uranium, compound fuel (LEU-COMP-THERM), because this is typical of most current-day reactors, and because these benchmarks were previously underpredicted by as much as 0.5% by most nuclear data libraries (such as ENDF/B-VI.8, JEFF-3.0). The calculated results presented here show that this underprediction is no longer there for ENDF/B-VII.0. The average over 257

  4. Clinical course of sly syndrome (mucopolysaccharidosis type VII)

    Science.gov (United States)

    Montaño, Adriana M; Lock-Hock, Ngu; Steiner, Robert D; Graham, Brett H; Szlago, Marina; Greenstein, Robert; Pineda, Mercedes; Gonzalez-Meneses, Antonio; Çoker, Mahmut; Bartholomew, Dennis; Sands, Mark S; Wang, Raymond; Giugliani, Roberto; Macaya, Alfons; Pastores, Gregory; Ketko, Anastasia K; Ezgü, Fatih; Tanaka, Akemi; Arash, Laila; Beck, Michael; Falk, Rena E; Bhattacharya, Kaustuv; Franco, José; White, Klane K; Mitchell, Grant A; Cimbalistiene, Loreta; Holtz, Max; Sly, William S

    2016-01-01

    Background Mucopolysaccharidosis VII (MPS VII) is an ultra-rare disease characterised by the deficiency of β-glucuronidase (GUS). Patients’ phenotypes vary from severe forms with hydrops fetalis, skeletal dysplasia and mental retardation to milder forms with fewer manifestations and mild skeletal abnormalities. Accurate assessments on the frequency and clinical characteristics of the disease have been scarce. The aim of this study was to collect such data. Methods We have conducted a survey of physicians to document the medical history of patients with MPS VII. The survey included anonymous information on patient demographics, family history, mode of diagnosis, age of onset, signs and symptoms, severity, management, clinical features and natural progression of the disease. Results We collected information on 56 patients from 11 countries. Patients with MPS VII were classified based on their phenotype into three different groups: (1) neonatal non-immune hydrops fetalis (NIHF) (n=10), (2) Infantile or adolescent form with history of hydrops fetalis (n=13) and (3) Infantile or adolescent form without known hydrops fetalis (n=33). Thirteen patients with MPS VII who had the infantile form with history of hydrops fetalis and survived childhood, had a wide range of clinical manifestations from mild to severe. Five patients underwent bone marrow transplantation and one patient underwent enzyme replacement therapy with recombinant human GUS. Conclusions MPS VII is a pan-ethnic inherited lysosomal storage disease with considerable phenotypical heterogeneity. Most patients have short stature, skeletal dysplasia, hepatosplenomegaly, hernias, cardiac involvement, pulmonary insufficiency and cognitive impairment. In these respects it resembles MPS I and MPS II. In MPS VII, however, one unique and distinguishing clinical feature is the unexpectedly high proportion of patients (41%) that had a history of NIHF. Presence of NIHF does not, by itself, predict the eventual severity

  5. Estudo da toxicidade de proteínas (Cry) recombinantes de Bacillus thuringiensis, utilizando o sistema de expressão baseado em baculovírus e células de inseto

    OpenAIRE

    Aguiar, Raimundo Wagner de Souza

    2007-01-01

    Os genes cry1Ca, cry2Ab e cry10Aa de diferentes estirpes brasileiras de Bacillus thuringiensis foram amplificados por PCR, clonados em um vetor de clonagem e seqüenciados. As análises das seqüências mostraram alta identidade com outros genes cry já descritos. Os genes foram removidos dos vetores de clonagem e introduzidos em um plasmídeo vetor de transferência (pSynXIVVI+X3) para construção de baculovírus recombinantes por recombinação homóloga. Os vírus recombinantes foram purificados por di...

  6. VII International Congress of Engineering Physics

    Science.gov (United States)

    2015-01-01

    In the frame of the fortieth anniversary celebration of the Universidad Autónoma Metropolitana and the Physics Engineering career, the Division of Basic Science and Engineering and its Departments organized the "VII International Congress of Physics Engineering". The Congress was held from 24 to 28 November 2014 in Mexico City, Mexico. This congress is the first of its type in Latin America, and because of its international character, it gathers experts on physics engineering from Mexico and all over the globe. Since 1999, this event has shown research, articles, projects, technological developments and vanguard scientists. These activities aim to spread, promote, and share the knowledge of Physics Engineering. The topics of the Congress were: • Renewable energies engineering • Materials technology • Nanotechnology • Medical physics • Educational physics engineering • Nuclear engineering • High precision instrumentation • Atmospheric physics • Optical engineering • Physics history • Acoustics This event integrates lectures on top trending topics with pre-congress workshops, which are given by recognized scientists with an outstanding academic record. The lectures and workshops allow the exchange of experiences, and create and strengthen research networks. The Congress also encourages professional mobility among all universities and research centres from all countries. CIIF2014 Organizing and Editorial Committee Dr. Ernesto Rodrigo Vázquez Cerón Universidad Autónoma Metropolitana - Azcapotzalco ervc@correo.azc.uam.mx Dr. Luis Enrique Noreña Franco Universidad Autónoma Metropolitana - Azcapotzalco lnf@correo.azc.uam.mx Dr. Alberto Rubio Ponce Universidad Autónoma Metropolitana - Azcapotzalco arp@correo.azc.uam.mx Dr. Óscar Olvera Neria Universidad Autónoma Metropolitana - Azcapotzalco oon@correo.azc.uam.mx Professor Jaime Granados Samaniego Universidad Autónoma Metropolitana - Azcapotzalco jgs@correo.azc.uam.mx Dr. Roberto Tito Hern

  7. Recombinant activated factor VII for uncontrolled bleeding postcardiac surgery

    Directory of Open Access Journals (Sweden)

    Aly Makram Habib

    2016-10-01

    Conclusion: In this analysis, rFVIIa succefully reduced the chest tube bleeding and blood products transfused during severe post cardiac surgical bleeding. However, safety of rFVIIa remains unclear. Prospective controlled trials are still needed to confirm the role of rFVIIa.

  8. (111)Indium Labelling of Recombinant Activated Coagulation Factor VII

    DEFF Research Database (Denmark)

    Nalla, Amarnadh; Buch, Inge; Sigvardt, Maibritt

    2012-01-01

    . administration of 1.6-1.8 MBq (111)In-DTPA-rFVIIa up to 120-130 min. Five min after administration of (111)In-DTPA-rFVIIa, percentage of (111)In activity was 6.0% in the cardiac region and 24.5% in the liver region. After 2 hours activity was decreased to 3.3% in heart while it had increased to 42...

  9. Apollonius de Perge, Coniques tome 4, livres VI et VII

    CERN Document Server

    Rashed, Roshdi

    2009-01-01

    Book VI of the Konika is essentially devoted to the question of the identity and similarity of two conic sections, or two parts of conic sections. In Book VII Apollonius deals with the various relationships between the lengths of diameters and conjugate diameters. The results are applied to the exposition of a number of problems, as well as to some problems which Apollonius indicates will be demonstrated and solved in Book VIII, which was lost in Antiquity. Books VI and VII have only survived in an Arabic translation, and are presented here in a critical edition, together with a faithful trans

  10. Conciliar politics and administration in the reign of Henry VII

    OpenAIRE

    Ford, Lisa L.

    2001-01-01

    Since Elton's commentary on the absence of critical study of the early Tudor council in 1964, some progress has been made towards a wider, fuller, more detailed understanding of Henry VII's council and where it fits-or does not-into the development of council under the Tudors. However, the early Tudor council remains something of an enigma. Added to that is recent interest by late medieval historians in just how much power Henry VII exercised in the operation of his councils. W...

  11. 40 CFR Appendix Vii to Part 86 - Standard Bench Cycle (SBC)

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 19 2010-07-01 2010-07-01 false Standard Bench Cycle (SBC) VII.... VII Appendix VII to Part 86—Standard Bench Cycle (SBC) 1. The standard bench aging durability... bench cycle (SBC) described in this appendix. 2. The SBC requires use of an aging bench with an...

  12. Incorporação do hormônio do crescimento humano recombinante (rhGH em matriz de polímero biodegradável

    Directory of Open Access Journals (Sweden)

    Ricardo Fernandes Garcia

    Full Text Available ResumoObjetivoIncorporar o hormônio de crescimento recombinante humano em um polímero biodegradável (PLGA.Material e métodoAs matrizes foram confeccionadas através da técnica de evaporação de solventes. Foi feita uma mistura do polímero (poli ácido glicólico lático e do hormônio do crescimento humano recombinante (Saizen® Merck Serono S.A. Aubonne, Suíça. Essa mistura foi vertida em moldes de silicone circulares de 1 cm de diâmetro e aproximadamente 2 mm de espessura, e levada para secagem em uma câmara de evaporação de solvente por 48 horas. Após esse período, as matrizes foram imersas em PBS e passaram por um banho termostatizado (ensaio de degradação hidrolítica, in vitro, à temperatura de 37°C. As amostras foram retiradas do banho no intervalo de 1, 2, 3, 4, 7, 10 e 14 dias. Foram aferidas a perda de massa, a variação do pH e a concentração do hormônio liberado em função do tempo.ResultadoA concentração do hormônio liberado em função do tempo foi aumentando até o terceiro dia. No quarto dia, houve uma queda e, no sétimo, ocorreu um aumento do hormônio liberado, estendendo-se até o décimo dia; no 14° dia, houve queda novamente. O pH teve uma queda brusca de 7,4 para 3,2 no primeiro dia, mantendo uma pequena queda até o 14° dia. A perda de massa foi gradual em relação ao tempo, como já era esperado.ConclusãoO PLGA é um bom biomaterial para confecção de matrizes com hormônio do crescimento. Revelou-se possível incorporar o rhGH nessa matriz, de modo a, então, desenvolver-se um substituto ósseo.

  13. Somatotropina bovina recombinante (rBST sobre o desempenho e a digestibilidade aparente de novilhas (½ Nelore x ½ Red Angus em confinamento

    Directory of Open Access Journals (Sweden)

    Nascimento Willian Gonçalves do

    2003-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da somatotropina bovina recombinante (rBST - análogo do BST, obtido comercialmente pela técnica do DNA recombinante, sobre ganho médio diário (GMD, ingestão de matéria seca (IMS, matéria orgânica (IMO, energia metabolizável (IEM e fibra em detergente neutro (IFDN, ingestão de matéria seca em relação ao peso vivo (IMS/PV, conversão alimentar (CA, rendimento de carcaça (RC e digestibilidade aparente da matéria seca (DAMS, matéria orgânica (DAMO, proteína bruta (DAPB, energia bruta (DAEB e fibra em detergente neutro (DAFDN. Foram utilizadas 24 novilhas mestiças (½ Nelore x ½ Red Angus, com aproximadamente 18 meses de idade e peso médio 255 kg. Os animais foram alimentados com uma dieta contendo silagem de milho, como volumoso, e polpa de citrus e farelo de soja, como concentrado, durante 84 dias. Essa dieta foi utilizada para os três tratamentos, que se diferenciaram pela aplicação de 250 mg de rBST, por via subcutânea, na fossa ísqueo-retal, onde: tratamento 1 controle (aplicação de dois mL de solução salina; 2 dose única e 3 uma dose a cada 14 dias. Os animais foram distribuídos em delineamento inteiramente casualizado, com três tratamentos e oito repetições. O GMD foi determinado a cada 14 dias. A ingestão de alimentos e a conversão da MS foram determinadas ao final do experimento. A digestibilidade aparente (DA foi determinada durante um período de sete dias com uso de indicador interno. Não houve diferença entre animais tratados ou não com rBST sobre o GMD, IMS, IPB, IEM, IMO e IFDN, CAMS e RC. No entanto, a DA da MS, MO, PB, EB e FND foi superior para as novilhas que receberam rBST a cada 14 dias.

  14. Viis armastuslugu suurel ekraanil / Tristan Priimägi

    Index Scriptorium Estoniae

    Priimägi, Tristan, 1976-

    2008-01-01

    Viis näidet filmiajaloost, kus režissööri ja staari vahel on tunded : Roger Vadim ja Tema Naised, Werner Herzog ja Klaus Kinski, Federico Fellini ja Anita Ekberg, Quentin Tarantino ja Uma Thurman, Rainer Werner Fassbinder ja Mr/Mrs X

  15. Viis aastakümmet raamatukogunduse radadel / Anne Veinberg

    Index Scriptorium Estoniae

    Veinberg, Anne

    2008-01-01

    Arvustus: Kikas, Konrad. Verba volant, scripta manent : viis aastakümmet raamatukogunduse ja infoteaduse radadel = Verba volant, scripta manent : five decades on the field of librarianship and information science. - Tallinn : Tallinna Tehnikaülikooli Kirjastus, 2008. - 366 lk. : ill. portr. - (Tallinna Tehnikaülikooli Raamatukogu töid. A ; 5)

  16. Kasuperest viidi viis last ja algatati kriminaalmenetlus piinamises / Rasmus Rekand

    Index Scriptorium Estoniae

    Rekand, Rasmus

    2011-01-01

    Lugu asenduskodu teenust pakkuvast perekonnast, kelle hoole all oli lisaks pere enda 3 lapsele 6 võõrast last ning kuidas lastega mittehakkama saamine viis olukorrani, kus lapsed viidi kasupere juurest ära ja lisaks algatati uurimine keelatud võtete kasutamise kohta kasvatamistegevuses

  17. Viis armastuslugu suurel ekraanil / Tristan Priimägi

    Index Scriptorium Estoniae

    Priimägi, Tristan, 1976-

    2008-01-01

    Viis näidet filmiajaloost, kus režissööri ja staari vahel on tunded : Roger Vadim ja Tema Naised, Werner Herzog ja Klaus Kinski, Federico Fellini ja Anita Ekberg, Quentin Tarantino ja Uma Thurman, Rainer Werner Fassbinder ja Mr/Mrs X

  18. Enrico VII e l’Italia. Un primo bilancio

    Directory of Open Access Journals (Sweden)

    Gian Maria Varanini

    2014-06-01

    Full Text Available Il testo introduce i saggi pubblicati nel fasc. 1 (2014 di “Reti Medievali - Rivista”, traccia un sintetico bilancio delle ricerche recenti sulla spedizione di Enrico VII di Lussemburgo in Italia (1310-1313 e propone alcune questioni interpretative.

  19. Subjective Criteria in Employment Decisions Under Title VII

    Science.gov (United States)

    Stacy, Donald R.

    1976-01-01

    Since higher echelon jobs have been drawn into litigation under Title VII, subjective criteria have been employed. The legal ramifications of assessing noncognitive traits such as leadership, aggressiveness, and attitude are discussed. Available from: the University of Georgia School of Law, Athens, Georgia 30602. (LBH)

  20. Viis aastakümmet raamatukogunduse radadel / Anne Veinberg

    Index Scriptorium Estoniae

    Veinberg, Anne

    2008-01-01

    Arvustus: Kikas, Konrad. Verba volant, scripta manent : viis aastakümmet raamatukogunduse ja infoteaduse radadel = Verba volant, scripta manent : five decades on the field of librarianship and information science. - Tallinn : Tallinna Tehnikaülikooli Kirjastus, 2008. - 366 lk. : ill. portr. - (Tallinna Tehnikaülikooli Raamatukogu töid. A ; 5)

  1. Speciation analysis of Mn(II)/Mn(VII) using Fe3O4@ionic liquids-β-cyclodextrin polymer magnetic solid phase extraction coupled with ICP-OES.

    Science.gov (United States)

    Chen, Songqing; Qin, Xingxiu; Gu, Weixi; Zhu, Xiashi

    2016-12-01

    Ionic liquids-β-cyclodextrin polymer (ILs-β-CDCP) was attached on Fe3O4 nanoparticles to prepare magnetic solid phase extraction agent (Fe3O4@ILs-β-CDCP). The properties and morphology of Fe3O4@ILs-β-CDCP were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction(XRD), size distribution and magnetic analysis. A new method of magnetic solid phase extraction (MSPE) coupled to ICP-OES for the speciation of Mn(II)/Mn(VII) in water samples was established. The results showed that Mn(VII) and total manganese [Mn(II)+Mn(VII)] were quantitatively extracted after adjusting aqueous sample solution to pH 6.0 and 10.0, respectively. Mn(II) was calculated by subtraction of Mn(VII) from total manganese. Fe3O4@ILs-β-CDCP showed a higher adsorption capacity toward Mn(II) and Mn(VII). Several factors, such as the pH value, extraction temperature and sample volume, were optimized to achieve the best extraction efficiency. Moreover, the adsorption ability of Fe3O4@ILs-β-CDCP would not be significantly lower after reusing of 10 times. The accuracy of the developed method was confirmed by analyzing certified reference materials (GSB 07-1189-2000), and by spiking spring water, city water and lake water samples. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. Calidad de las actividades de la asignatura medicina general integral VII en los diferentes escenarios docentes

    Directory of Open Access Journals (Sweden)

    Maricel Felicia Castellanos González

    2012-06-01

    Full Text Available Fundamento: en el proceso de enseñanza-aprendizaje que se desarrolla en los diferentes escenarios docentes, se deben tener en cuenta los contenidos ya impartidos, para logar una integración entre los conocimientos teóricos con la práctica médica. Objetivo: evaluar los escenarios docentes según la calidad de las actividades desarrolladas en la asignatura medicina general integral VII, en el contexto del policlínico universitario. Método: se realizó una investigación cuanti-cualitativa según el cumplimiento de indicadores de calidad mediante la aplicación de una guía de observación en los escenarios docentes de la asignatura medicina general integral VII, en el policlínico universitario. Los escenarios docentes evaluados fueron: consultorios, vacunatorios, círculos infantiles, hogares maternos y consulta de planificación familiar. Resultados: el consultorio, el vacunatorio, la consulta de planificación familiar, la escuela y círculo infantil fueron evaluados de regular, solo obtuvieron categoría de bien el hogar materno y el escenario cuerpo de guardia. Las actividades docentes con mayores dificultades fueron: la vigilancia epidemiológica para la pesquisa de factores de riesgo, el control de foco para enfermedades transmisibles y la presentación del análisis de la situación de salud a la comunidad. Conclusiones: el nivel escenario docente en la asignatura medicina general integral VII, de forma general fue evaluado de regular, por tener: el escenario consultorio, el vacunatorio, consulta de planificación familiar, la escuela y círculo infantil evaluados de regular, el hogar materno y el escenario cuerpo de guardia evaluados de bien.

  3. EVALUACIÓN DE LA PRODUCCIÓN DE ETANOL UTILIZANDO CEPAS RECOMBINANTES DE Saccharomyces cerevisiae A PARTIR DE MELAZA DE CAÑA DE AZÚCAR

    Directory of Open Access Journals (Sweden)

    CAROLINA PEÑA

    2009-01-01

    Full Text Available Se evaluó la producción de etanol, el crecimiento celular y el consumo de sustrato de tres cepas de Saccharomyces cerevisiae: CBS8066 (control y dos recombinantes desarrolladas en la Unidad de Biotecnología Vegetal de CIB, GG570-CIBI y GG570-CIBII. Dichas cepas estuvieron bajo el efecto de dos concentraciones de sacarosa (170 y 250g/L y dos sustratos (industrial con melaza caña azúcar y sintético con sacarosa. Durante la fermentación en sustrato industrial se obtuvo mayor producción de etanol a concentración de 250g sacarosa/L. Bajo estas condiciones, la cepa GG570-CIBII produjo en promedio 2,34g etanol/L mas con respecto a la cepa control y en adición, a las 10h, produjo 8,02g/L por encima de la cepa control. Por otro lado, la cepa GG570- CIBI produjo 3,46g etanol/L menos que la cepa control. De esta forma, se encontró que la cepa GG570-CIBII es tolerante a alta concentración de sacarosa, y además, es capaz de producir una concentración mayor de etanol con respecto a la cepa control en melaza caña azúcar con 250g/L sacarosa.

  4. Fermentation of sweet whey by recombinant Escherichia coli K011 Fermentação de soro de leite por Escherichia coli KO11 recombinante

    Directory of Open Access Journals (Sweden)

    Amarildo Ricardo Leite

    2000-09-01

    Full Text Available The production of ethanol from sweet whey using the recombinant Escherichia coli KO11, in batch fermentation, was tested. The maximum ethanol yield was reached after 96h, representing only 38% of the theoretical yield. The supplementation of whey with components of LB broth increased the maximum yield to 96% in 72h. The addition of 0.5% yeast extract to whey resulted in maximum yield of 74% at 36h and it increased to over 100% when yeast extract and trace metals solution (Fe++, Mn++ and Zn++ were added.A produção de etanol a partir de soro de leite empregando a cepa Escherichia coli KO11 recombinante, em fermentação de batelada, foi testada. O rendimento máximo de etanol foi obtido em 96h, representando apenas 38% do rendimento teórico. A suplementação do soro com os componentes do caldo LB aumentou o rendimento para 96% em 72h. A adição de 0,5% de extrato de levedura ao soro resultou em um rendimento máximo de 74% em 36h que aumentou para acima de 100% quando se adicionou extrato de levedura e uma solução de metais traço (Fe++, Mn++ e Zn++.

  5. Vanillin production by recombinant strains of Escherichia coli Produção de vanilina por linhagens recombinantes de Escherichia coli

    Directory of Open Access Journals (Sweden)

    Attilio Converti

    2003-11-01

    Full Text Available Vanillin production from ferulate was studied using different recombinant strains of Escherichia coli. To prevent the occurrence of aerobic conditions and then possible product oxidation, tests were performed in Erlenmeyer flasks under mild mixing (150 rpm. Among other transformants, E. coli JM109(pBB1 appeared to be the best vanillin producer, being able to convert no less than 95% of starting ferulate to the product within 1h. This yield decreased down to 72% after 72h, likely because of a non-specific oxidase activity responsible for vanillin oxidation to vanillate.A produção de vanilina a partir de ácido ferúlico foi estudada utilizando-se diferentes linhagens recombinantes de Escherichia coli. Para prevenir a ocorrência de condições de aerobiose e a possível oxidação do produto, os ensaios foram realizados em frascos Erlenmeyer sob agitação moderada (150 rpm. E. coli JM109 (pBBI mostrou-se o melhor produtor de vanilina entre os demais agentes transformantes, sendo capaz de converter 95% do ácido ferúlico inicial em produto após 1h, rendimento este que decresceu para 72% após 72h, provavelmente devido à atividade de uma oxidase não-específica responsável pela oxidação de vanilina a ácido vanílico.

  6. 40 CFR Appendix Vii to Part 266 - Health-Based Limits for Exclusion of Waste-Derived Residues*

    Science.gov (United States)

    2010-07-01

    ... Waste-Derived Residues* VII Appendix VII to Part 266 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR THE MANAGEMENT OF SPECIFIC HAZARDOUS WASTES AND SPECIFIC TYPES OF HAZARDOUS WASTE MANAGEMENT FACILITIES Pt. 266, App. VII Appendix VII to Part 266—Health...

  7. The Geometrical Structure of the Tolman VII solution

    CERN Document Server

    Raghoonundun, Ambrish M

    2016-01-01

    The Tolman~VII solution, an exact analytic solution to the spherically symmetric, static Einstein equations with a perfect fluid source, has many characteristics that make it interesting for modelling high density physical astronomical objects. Here we supplement those characteristics with the geometrical tensors that this solution possess, and find that the Weyl, Riemann, and Ricci tensor components show unexpected mathematical behaviour that change depending on physically motivated parameters, even though the mass of the modelled objects is fixed. We show these features firstly through tensor components, and then through the scalars in the null tetrad formalism of Newmann and Penrose. The salient conclusion of this analysis is the intimate relationship between the Tolman~VII solution and the constant density Schwarzschild interior solution: the former being a straight forward generalization of the latter while eschewing the unphysical constant density.

  8. Diagnóstico de ABPA em pacientes portadores de fibrose cística: utilidade clínica da pesquisa de IgE específica contra alérgenos recombinantes do Aspergillus fumigatus ABPA diagnosis in cystic fibrosis patients: the clinical utility of IgE specific to recombinant Aspergillus fumigatus allergens

    Directory of Open Access Journals (Sweden)

    Marina B. Almeida

    2006-06-01

    Full Text Available OBJETIVO: A aspergilose broncopulmonar alérgica (ABPA é um fator complicador da fibrose cística que pode determinar uma combinação devastadora na evolução da doença pulmonar. A sobreposição de sinais e sintomas das duas enfermidades dificulta o diagnóstico, mesmo aplicando critérios padronizados. O objetivo deste trabalho foi identificar, em grupo de portadores de fibrose cística, os casos de ABPA através da detecção de IgE específica contra os alérgenos recombinantes do Aspergillus fumigatus e confrontar esse método com os critérios preconizados pela Cystic Fibrosis Foundation. MÉTODOS: Cinqüenta e quatro pacientes de 2 a 20 anos, com características que poderiam estar isoladamente presentes na ABPA, foram avaliados sistematicamente, incluindo: dados clínicos, tomografia computadorizada de tórax, teste cutâneo de hipersensibilidade imediata para A. fumigatus; dosagem de IgE sérica total, RAST para A. fumigatus, e IgE sérica específica para alérgenos recombinantes r Asp f1, f2, f3, f4 e f6. RESULTADOS: Foram elegíveis para o estudo 39 pacientes. Destes, 32 foram investigados. Houve sensibilização ao A. fumigatus em 34%. Ambos os métodos, o critério da Cystic Fibrosis Foundation e a pesquisa de IgE específica contra antígenos recombinantes, determinaram três casos de ABPA; entretanto, o diagnóstico foi concordante em apenas dois pacientes. CONCLUSÃO: A detecção de IgE específica contra antígenos recombinantes do A. fumigatus foi ferramenta útil para detecção precoce da sensibilização e diagnóstico de ABPA. No entanto, a confirmação diagnóstica não pôde ser desvinculada da condição clínica, e sua utilização para diagnóstico, detecção de recidivas e critério de cura ainda requer estudos longitudinais, envolvendo maior número de pacientes.OBJECTIVE: Allergic bronchopulmonary aspergillosis (ABPA is a complicating factor of cystic fibrosis which can result in a devastating combination as

  9. Validación de un ELISA para la cuantificación de las impurezas proteicas de la cepa hospedera en el principio activo de la vacuna recombinante cubana contra la hepatitis B

    OpenAIRE

    Eliana Pérez; Susset Valderrama; Alain Baxera; Yunaisy Jiménez; Gerardo García; Lourdes Costa; Marisel Quintana

    2014-01-01

    Se validó un inmunoensayo tipo sandwich de doble anticuerpo para cuantificar las impurezas proteicas de la cepa hospedera que pueden estar presentes en el principio activo de la vacuna cubana contra la hepatitis B. Se prepararon los reactivos biológicos empleados en el ELISA. Las proteínas de la cepa hospedera se obtuvieron bajo las mismas condiciones que el proceso de producción de la proteína recombinante hasta un paso de semipurificación o purificación primaria. Los antisueros dirigidos co...

  10. Comparación de la antigenicidad de dos péptidos sintéticos y una proteína recombinante de la región de transmembrana (gp21 del HTLV-I

    Directory of Open Access Journals (Sweden)

    Milenen Hernández Marin

    2007-01-01

    Full Text Available El virus de la leucemia de las células T humanas tipo I (HTLV-I fue el primer retrovirus linfotrópico aislado de pacientes humanos, en 1980. El gen env codifica la síntesis de la proteína precursora, gp62, que es degradada por una proteasa del virus en dos proteínas: gp21 y gp46, las cuales son expresadas en la superficie de la célula. La proteína de la transmembrana gp21 resulta una de las proteínas más antigénicas del virus del HTLV. Esta proteína, obtenida por la tecnología del ADN recombinante o por síntesis química, es ampliamente usada como antígeno en el diagnóstico del HTLV-I. En el presente trabajo, se evaluó la antigenicidad de dos péptidos sintéticos gp21 (M-1 (377-400 y gp21 (13 (361-404 y una proteína recombinante de la región de la transmembrana (gp21 del HTLV-I, con 23 muestras positivas del Panel PRP-205 (Boston Biomedica Inc y cinco muestras de pacientes seropositivos cubanos al HTLV-I, todas las muestras utilizadas en el estudio fueron confirmadas por el ensayo de Western Blot. La especificidad de estos antígenos se evaluó con 30 muestras de sueros de donantes sanos de un banco de sangre. Se obtuvo 62 % y 92 % de reactividad para los péptidos sintéticos gp21 (M-1, gp21 (13 y un 85 % para la proteína recombinante, respectivamente. La especificidad de los antígenos fue de 100 %. Este estudio demostró la alta reactividad de los péptidos sintéticos comparado con la proteína recombinante.

  11. Efecto de la hormona de crecimiento recombinante humana en la regeneración de nervio periférico: Trabajo experimental en el nervio cubital de la rata

    OpenAIRE

    Saceda Gutiérrez, Javier Manuel

    2016-01-01

    Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Cirugía. Fecha de lectura: Junio - 2016 Cuando se producen lesiones en los nervios periféricos se pueden utilizar diferentes sustancias o productos para intentar mejorar su crecimiento y reparación. En el presente trabajo valoramos la efectividad de la hormona de crecimiento recombinante humana (Somatropina, GH) en la lesión del nervio periférico de la rata Wistar. Mediante técnic...

  12. Vectores recombinantes basados en el virus modificado de Ankara (MVA), con deleción en el gen C6L, como vacunas contra el VIH/SIDA y otras enfermedades

    OpenAIRE

    García-Arriaza, J.; Gómez, Carmen E.; Esteban, Mariano

    2011-01-01

    La presente invención se engloba dentro de los campos de la biología molecular y de la biotecnología. Específicamente se refiere a virus recombinantes basados en el virus modificado de Ankara (MVA) que expresan los antígenos gp120 y Gag-Pol-Nef del virus de la inmunodeficiencia humana (VIH-1) de subtipo B (MVA-B), sobre los que se ha delecionado el gen de vaccinia C6L, y que han sido diseñados para utilizarse como vacunas contra el VIH/SIDA y otras enfermedades.

  13. Vectores recombinantes basados en el virus modificado de ankara (MVA), con deleción en el gen C6L, como vacunas contra el VIH/SIDA y otras enfermedades

    OpenAIRE

    García-Arriaza, J.; Gómez, Carmen E.; Esteban, Mariano

    2011-01-01

    [ES] La presente invención engloba dentro de los campos de la biología molecular y de la biotecnología. Especificamente se refiere a virus recombinantes basados en el virus modificado de Ankara (MVA) que expresan los antigenos gp120 y Gag-Pol-Nef del virus de la inmunodeficienciahumana (VIH-1) de subtipo B (MVA-B), sobre los que see ha delecionado el gen de vaccinia C6L, y que han sido diseñados para utilizarse como vacunas contra el VIH/SIDA y otras enfermedades.

  14. CROSS SECTION EVALUATIONS FOR ENDF/B-VII.

    Energy Technology Data Exchange (ETDEWEB)

    HERMAN, M.; ROCHMAN, D.; OBLOZINSKY, P.

    2006-06-05

    This is the final report of the work performed under the LANL contract on neutron cross section evaluations for ENDF/B-VII (April 2005-May 2006). The purpose of the contract was to ensure seamless integration of the LANL neutron cross section evaluations in the new ENDF/B-VII library. The following work was performed: (1) LANL evaluated data files submitted for inclusion in ENDF/B-VII were checked and, when necessary, formal formatting errors were corrected. As a consequence, ENDF checking codes, run on all LANL files, do not report any errors that would rise concern. (2) LANL dosimetry evaluations for {sup 191}Ir and {sup 193}Ir were completed to match ENDF requirements for the general purpose library suitable for transport calculations. A set of covariances for both isotopes is included in the ENDF files. (3) Library of fission products was assembled and successfully tested with ENDF checking codes, processed with NJOY-99.125 and simple MCNP calculations. (4) KALMAN code has been integrated with the EMPIRE system to allow estimation of covariances based on the combination of measurements and model calculations. Covariances were produced for 155,157-Gd and also for 6 remaining isotopes of Gd.

  15. Impact of ENDF/B-VII.0 for AECL applications

    Energy Technology Data Exchange (ETDEWEB)

    Kozier, Ken S.; Altiparmakov, Dimitar V. [AECL - Chalk River Laboratories, Chalk River (Canada)

    2008-07-01

    This paper examines the impact of the new evaluated nuclear data library ENDF/B-VII.0 on selected reactor physics applications at AECL. The twin objectives are to provide feedback to the nuclear data community concerning the practical impact of their work and preliminary guidance to end-users. This work is based on comparison of the results of MCNP simulations with critical measurements involving both the ZED-2 zero power reactor and the MAPLE dedicated isotope production reactors at the Chalk River Laboratories. Significant improvement in the reactivity agreement with the measurements is obtained with ENDF/B-VII.0 for the specific ZED-2 measurements analysed; however, improvements associated with the thermal scattering law data for UO{sub 2} that had been observed initially were subsequently determined to be fortuitous, due to the inadvertent omission of the elastic neutron scattering component. Additionally, the net reactivity impact of major changes to the {sup 90}Zr and {sup 91}Zr capture cross sections with ENDF/B-VII.0 is examined in the MAPLE reactor context and found to be modest due primarily to the offsetting effects of the specific nuclides involved. (authors)

  16. Potassium chloride-bearing ice VII and ice planet dynamics

    Science.gov (United States)

    Frank, Mark R.; Scott, Henry P.; Aarestad, Elizabeth; Prakapenka, Vitali B.

    2016-02-01

    Accurate modeling of planetary interiors requires that the pressure-volume-temperature (PVT) properties of phases present within the body be well understood. The high-pressure polymorphs of H2O have been studied extensively due to the abundance of ice phases in icy moons and, likely, vast number of extra-solar planetary bodies, with only select studies evaluating impurity-laden ices. In this study, ice formed from a 1.6 mol percent KCl-bearing aqueous solution was studied up to 32.89 ± 0.19 GPa and 625 K, and the incorporation of K+ and Cl- ionic impurities into the ice VII structure was documented. The compression data at 295 K were fit with a third order Birch-Murnaghan equation of state and yielded a bulk modulus (KT0), its pressure derivative (KT0‧), and zero pressure volume (V0) of 24.7 ± 0.9 GPa, 4.44 ± 0.09, and 39.2 ± 0.2 Å3, respectively. The impurity-laden ice was found to be 6-8% denser than ice VII formed from pure H2O. Thermal expansion coefficients were also determined for several isothermal compression curves at elevated temperatures, and a PVT equation of state was obtained. The melting curve of ice VII with incorporated K+ and Cl- was estimated by fitting experimental data up to 10.2 ± 0.4 GPa, where melting occurred at 625 K, to the Simon-Glatzel equation. The melting curve of this impurity-laden ice is systematically depressed relative to that of pure H2O by approximately 45 K and 80 K at 4 and 11 GPa, respectively. A portion of the K+ and Cl- contained within the ice VII structure was observed to exsolve with increasing temperature. This suggests that an internal differentiating process could concentrate a K-rich phase deep within H2O-rich planets, and we speculate that this could supply an additional source of heat through the radioactive decay of 40K. Our data illustrate ice VII can incorporate significant concentrations of K+ and Cl- and increasing the possibility of deep-sourced and solute-rich plumes in moderate to large sized H2O

  17. Optimización de una técnica de medida de disrupción endocrina por medio de Saccharomyces cerevisiae recombinantes

    Directory of Open Access Journals (Sweden)

    Karen Keel

    2011-05-01

    Full Text Available Los disruptores endocrinos son sustancias químicas, exógenas al organismo, capaces de alterar la homeostasis del sistema endocrino-reproductivo. Un grupo de ellos, los compuestos estrogénicos, pueden ocasionar efectos negativos sobre la capacidad reproductiva de algunos peces, disminución de la fertilidad, la aptitud sexual y la producción de esperma y feminización de los machos. En este trabajo se describe el desempeno analítico de un método para la cuantificación de estrogenicidad en aguas. Se utilizó la técnica Yeast Estrogen Screen (YES con Saccharomyces cerevisiae recombinante, una levadura modificada genéticamente que expresa el receptor de estrógenos humano y un plasmido de expresión con el gen reportero lacZ, bajo el control de elementos de respuesta a estrógenos. Dicho gen codifica para la ƒÀ-galactosidasa, que se secreta al medio y metaboliza un sustrato cromogénico, observandose un cambio de color que se mide a 420 nm. Se utilizaron estándares de 17 ƒÀ-estradiol para realizar una curva de calibración en el rango de concentraciones entre 1.75 ng/l y 7.5 ƒÊg/l. Las curvas sigmoides dosis.respuesta obtenidas se ajustaron mediante la función de Hill y los r2 fueron mayores a 0,95. El limite de detección fue de 55 ng/l. La estrogenicidad para las muestras ensayadas fue no detectable.

  18. Teste intradérmico com proteínas recombinantes de Mycobacterium bovis como antígenos em Cavia porcellus

    Directory of Open Access Journals (Sweden)

    Elaine S.P. Melo

    2014-10-01

    Full Text Available O teste intradérmico para o diagnóstico da tuberculose bovina utiliza derivados proteicos purificados (PPD de Mycobacterium bovis que são capazes de induzir reações de hipersensibilidade em animais infectados. No entanto, apresenta baixa especificidade devido à ocorrência de reações cruzadas com outras micobactérias. Neste sentido, o objetivo desse trabalho foi produzir proteínas recombinantes (ESAT-6, PE13, PE5 e ESX-1 de Mycobacterium bovis e avaliá-las como antígenos em teste intradérmico utilizando Cavia porcellus como modelo, e verificar se as condições empregadas na purificação (nativa ou desnaturante interferem no desempenho antigênico dessas proteínas. As proteínas foram testadas em Cavia porcellus previamente sensibilizados com cepa M. bovis AN5 inativada, individualmente (160 µg ou combinadas na forma de um coquetel (40 µg cada. O coquetel de proteínas induziu reações de hipersensibilidade nos animais sensibilizados significativamente superiores (p=0,002 as observadas nos animais não sensibilizados, possibilitando diferenciação. No entanto, as proteínas isoladamente não foram capazes de promover essa diferenciação. As condições de solubilização e purificação influenciaram o desempenho antigênico da proteína ESAT-6, pois, quando produzida em condição desnaturante desencadeou reações inespecíficas nos animais não sensibilizados, enquanto que aquela produzida em condições nativas e aplicada em concentrações de 6, 12, 24 e 48µg induziu reações significativas apenas nos animais sensibilizados, confirmando o seu potencial como antígeno.

  19. Alternative splicing isoforms of synaptotagmin VII in the mouse, rat and human.

    Science.gov (United States)

    Fukuda, Mitsunori; Ogata, Yukie; Saegusa, Chika; Kanno, Eiko; Mikoshiba, Katsuhiko

    2002-07-01

    Synaptotagmin VII (Syt VII) has been proposed to regulate several different types of Ca2+-dependent exocytosis, but its subcellular localization (lysosome or plasma membrane) and the number of alternative splicing isoforms of Syt VII (single or multiple forms) are matters of controversy. In the present study, we show by reverse transcriptase-PCR analysis that mouse Syt VII has one major isoform (Syt VIIalpha), the original Syt VII, and two minor isoforms (Syt VIIbeta and Syt VIIgamma), which contain unique insertions (of 44 and 116 amino acids respectively) in the spacer domain between the transmembrane and C2 domains of Syt VIIalpha. Similar results were obtained with respect to rat and human Syt VII mRNA expression. An antibody against the N-terminal domain of mouse Syt VII [anti-(Syt VII-N)], which specifically recognized recombinant Syt VII but not other Syt isoforms expressed in COS-7 cells, recognized two major, closely co-migrating bands (p58 and p60) and minor bands of approx. 65 kDa in mouse brain. Immunoaffinity purification of proteins that bind the anti-(Syt VII-N) antibody, and peptide sequence analysis revealed that: (i) the major p58 and p60 bands are identified as adenylate cyclase-associated protein 2; (ii) actin-binding protein is localized at the plasma membrane; and (iii) Syt VIIalpha (65 kDa) is the major Syt VII isoform, but with a much lower expression level than previously thought. It was also shown that FLAG-Syt VII-green-fluorescence-protein fusion protein stably expressed in PC12 cells is localized in the perinuclear region (co-localization with TGN38 protein, even after brefeldin A treatment) and in the tips of neurites (co-localization with Syt I), and not in the plasma membrane.

  20. Uudised : Baltimaade suurim, XII "Jazzkaar". VII rahvusvaheline koorifestival. Leipzigis improviseeriti edukalt

    Index Scriptorium Estoniae

    2001-01-01

    23.-29. apr. toimuvast festivalist "Jazzkaar 2001". VII rahvusvahelisest koorifestivalist "Tallinn "2001". 6. ja 7. apr. Leipzigis toimunud V rahvusvahelisest improvisatsioonikonkursist, kus osalesid ka eesti interpreedid

  1. Uudised : Baltimaade suurim, XII "Jazzkaar". VII rahvusvaheline koorifestival. Leipzigis improviseeriti edukalt

    Index Scriptorium Estoniae

    2001-01-01

    23.-29. apr. toimuvast festivalist "Jazzkaar 2001". VII rahvusvahelisest koorifestivalist "Tallinn "2001". 6. ja 7. apr. Leipzigis toimunud V rahvusvahelisest improvisatsioonikonkursist, kus osalesid ka eesti interpreedid

  2. Result of Rendezvous Docking Experiment of ETS.35zw .5zw .25mmVII

    Science.gov (United States)

    Kawano, Isao; Mokuno, Masaaki; Suzuki, Takashi; Koyama, Hiroshi; Kunugi, Makoto

    ETS VII is a test satellite to perform in-orbit demonstration of autonomous rendezvous docking (RVD) technology, which will be necessary for advanced space activities in the early 21st century. ETS VII performed three RVD experiment flights, and verified all technical items. ETS VII demonstrated first autonomous RVD between unmanned vehicles, and remote piloted rendezvous flight position accuracy at docking was about 1cm, and acceleration was less than 1.5mG (low impact docking). In the second RVD experiment flight, ETS VII detected attitude anomaly and executed disable abort for safety insurance. We present the results and evaluation of three RVD experiment flights in this paper.

  3. Pengembangan Vaksin Inaktif Tetelo Genotipe VII Isolat Lokal pada Kondisi Laboratorium. (DEVELOPMENT OF TETELO INACTIVATED VACCINE GENOTYPE VII LOCAL ISOLATE IN LABORATORY CONDITION

    Directory of Open Access Journals (Sweden)

    Risa Indriani

    2016-11-01

    Full Text Available Tetelo/Newcastle disease (ND inactive vaccine of genotipe VII virus local isolate have been developedin laboratory condition and compared with commercial ND vaccine. A total of 200 commercial layer chickenat 4 weeks age were divided into four groups, that were (1 vaccinated with ND genotype VII Indonesia/GTT/11, (2 vaccinated with commercial ND vaccine genotype VII, (3 vaccinated with commercial genotypeVI and (4 unvaccinated as control group. After two weeks post vaccination, 10 chicken from each groupwere sellected randomly and challenged with 105 EID50 per 0,1 mL of ND virus genotype VII Indonesian/GTT/11 by intramuscular. Chicken were observed, and swab were collected from oropharyngeal and cloacaat 2, 5, 7, 12 and 14 days post challenge. The result of this study showed inactived vaccine genotype VIIIndonesia/GTT/11 can induced a good antibody titer response to vaccinated chicken with mean titer 7.30log2 and CI 6.3 to 7.8, while commercial ND vaccine genotipe VII was 5.30 log2 with CI 3.8-6.7, andcommercial genotype VI was 4.8 log2 with CI 4.1-5.4. The level of protection which determined by noclinical signs, mortality and viral shedding showed 100% protection in chicken vaccinated with Indonesia/GTT/11 and commercial genotype VII were 100%, compared with control chicken, and vaccined commercialND vaccine genotype VII, compared with control chicken. While in chicken vaccinated commercial NDvaccine genotype VI showed viral shedding on day two post challenge, but there were no clinical sign andmortality. Based on this results, Indonesia/GTT/11 genotype VII ND vaccine could be used as an alternativeND vaccine to protect chicken from infection of ND virus genotype VII in the field.

  4. Stellarator Wendelstein VII-AS; Physics and engineering design

    Energy Technology Data Exchange (ETDEWEB)

    Sapper, J.; Renner, H. (Max-Planck-Institut fuer Plasmaphysik, Garching (Germany, F.R.))

    1990-01-01

    The advanced stellarator Wendelstein VII-AS is a medium-sized experimental machine with the following properties and aims: a broadly optimized magnetic configuration with improved plasma equilibrium and smaller neoclassical transport losses compared to classical stellarators; operation with net-current-free plasma; field generation with a modular set of coils instead of the conventional helix/toroidal field coil; system; and good access for plasma heating methods, e.g., neutral beam injection, electron cyclotron heating, and ion cyclotron heating. The physics characteristics and the engineering design of the experiment as well as results from the initial operation period are reported.

  5. Methods for Processing ENDF/B-VII with NJOY

    Science.gov (United States)

    MacFarlane, R. E.; Kahler, A. C.

    2010-12-01

    The NJOY Nuclear Data Processing System is widely used to convert evaluations in the Evaluated Nuclear Data Files (ENDF) format into forms useful for practical applications such as fission and fusion reactor analysis, stockpile stewardship calculations, criticality safety, radiation shielding, nuclear waste management, nuclear medicine procedures, and more. This paper provides a description of the system's capabilities, summary descriptions of the methods used, and information on how to use the code to process the modern evaluated nuclear data files from ENDF/B-VII. It begins with the generation of pointwise libraries, including reaction and resonance reconstruction, Doppler broadening, radiation heating and damage, thermal scattering data, unresolved resonance data, and gas production. It then reviews the production of libraries for the continuous-energy Monte Carlo code MCNP, multigroup neutron, photon, and particle cross sections and matrices, and photon interaction data. The generation of uncertainty information for ENDF data is discussed, including new capabilities for calculating covariances of resonance data, angular distributions, energy distributions, and radioactive nuclide production. NJOY's ability to prepare thermal scattering data evaluations for bound moderators (which was used during the preparation of the ENDF/B-VII library) is described. The strong plotting capabilities of NJOY are summarized. Many examples of black&white and color Postscript plots are included throughout the paper. The capabilities of NJOY to output multigroup data in several different formats to suit various applications is reviewed. Finally, a section is included that summarizes the history of the development of the NJOY system over the last 37 years.

  6. Delayed presentation of traumatic facial nerve (CN VII) paralysis.

    Science.gov (United States)

    Napoli, Anthony M; Panagos, Peter

    2005-11-01

    Facial nerve paralysis (Cranial Nerve VII, CN VII) can be a disfiguring disorder with profound impact upon the patient. The etiology of facial nerve paralysis may be congenital, iatrogenic, or result from neoplasm, infection, trauma, or toxic exposure. In the emergency department, the most common cause of unilateral facial paralysis is Bell's palsy, also known as idiopathic facial paralysis (IFP). We report a case of delayed presentation of unilateral facial nerve paralysis 3 days after sustaining a traumatic head injury. Re-evaluation and imaging of this patient revealed a full facial paralysis and temporal bone fracture extending into the facial canal. Because cranial nerve injuries occur in approximately 5-10% of head-injured patients, a good history and physical examination is important to differentiate IFP from another etiology. Newer generation high-resolution computed tomography (CT) scans are commonly demonstrating these fractures. An understanding of this complication, appropriate patient follow-up, and early involvement of the Otolaryngologist is important in management of these patients. The mechanism as well as the timing of facial nerve paralysis will determine the proper evaluation, consultation, and management for the patient. Patients with total or immediate paralysis as well as those with poorly prognostic audiogram results are good candidates for surgical repair.

  7. Dolor lumbar por estreptoquinasa recombinante: Presentación de un caso Lumbar pain caused by recombinant streptokinase: A case presentation

    Directory of Open Access Journals (Sweden)

    María Julia Cigales Reyes

    2009-08-01

    Full Text Available La Heberkinasa® (estreptoquinasa recombinante, es un trombolítico utilizado en el tratamiento del infarto agudo del miocardio, presentada en forma de liofilizado en bulbos estériles, de conocidos y probados efectos de reperfusión coronaria y reducción del tamaño del infarto, además de otras aplicaciones como en la trombosis venosa profunda, trombosis de acceso vascular permanente de pacientes con insuficiencia renal crónica terminal tratados por hemodiálisis periódicas, disfunción de prótesis valvulares cardíacas por trombos y en el tromboembolismo pulmonar; se asocian reacciones adversas durante el tratamiento, frecuentes y menos frecuentes, dentro de las que se encuentra el dolor lumbar. Se presenta a una paciente con el diagnóstico de un infarto agudo del miocardio, de cara diafragmática, a quien se le aplicó la trombolisis con Heberkinasa y durante esta presentó dolor lumbar agudo, intenso, que cedió con la reducción de goteo de la infusión y esta se pudo continuar sin más problemas.The Heberkinase® (recombinant streptokinase is a thrombolytic agent used in treatment of acute myocardial infarction, presented as sterile bulbs, of known and proved effects of coronary reperfusion and reduction of infarction dimension, besides of other applications e.g. the deep venous thrombosis, permanent vascular access thrombosis in patients presenting with terminal chronic renal insufficiency treated by periodical hemodialysis, dysfunction of cardiac vascular prostheses by thrombi, and in case of pulmonary thromboembolism; there are adverse reactions associated during treatment, frequent and less frequent including those of lumbar pain. Authors present a case of a woman diagnosed with acute myocardial infarction of diaphragmatic side undergoing thrombolysis with Heberkinase, and during it she had intense and acute lumbar pain improving with reduction of dripping infusion without subsequent problems.

  8. Title VII Funding and Physician Practice in Rural or Low-Income Areas

    Science.gov (United States)

    Krist, Alex H.; Johnson, Robert E.; Callahan, David; Woolf, Steven H.; Marsland, David

    2005-01-01

    Whether Title VII funding enhances physician supply in underserved areas has not clearly been established. The purpose was to determine the relation between Title VII funding in medical school, residency, or both, and the number of family physicians practicing in rural or low-income communities. A retrospective cross sectional analysis was carried…

  9. Adenovirus Core Protein pVII Is Translocated into the Nucleus by Multiple Import Receptor Pathways†

    Science.gov (United States)

    Wodrich, Harald; Cassany, Aurelia; D'Angelo, Maximiliano A.; Guan, Tinglu; Nemerow, Glen; Gerace, Larry

    2006-01-01

    Adenoviruses are nonenveloped viruses with an ∼36-kb double-stranded DNA genome that replicate in the nucleus. Protein VII, an abundant structural component of the adenovirus core that is strongly associated with adenovirus DNA, is imported into the nucleus contemporaneously with the adenovirus genome shortly after virus infection and may promote DNA import. In this study, we evaluated whether protein VII uses specific receptor-mediated mechanisms for import into the nucleus. We found that it contains potent nuclear localization signal (NLS) activity by transfection of cultured cells with protein VII fusion constructs and by microinjection of cells with recombinant protein VII fusions. We identified three NLS-containing regions in protein VII by deletion mapping and determined important NLS residues by site-specific mutagenesis. We found that recombinant protein VII and its NLS-containing domains strongly and specifically bind to importin α, importin β, importin 7, and transportin, which are among the most abundant cellular nuclear import receptors. Moreover, these receptors can mediate the nuclear import of protein VII fusions in vitro in permeabilized cells. Considered together, these data support the hypothesis that protein VII is a major NLS-containing adaptor for receptor-mediated import of adenovirus DNA and that multiple import pathways are utilized to promote efficient nuclear entry of the viral genome. PMID:16973564

  10. Adenovirus core protein pVII is translocated into the nucleus by multiple import receptor pathways.

    Science.gov (United States)

    Wodrich, Harald; Cassany, Aurelia; D'Angelo, Maximiliano A; Guan, Tinglu; Nemerow, Glen; Gerace, Larry

    2006-10-01

    Adenoviruses are nonenveloped viruses with an approximately 36-kb double-stranded DNA genome that replicate in the nucleus. Protein VII, an abundant structural component of the adenovirus core that is strongly associated with adenovirus DNA, is imported into the nucleus contemporaneously with the adenovirus genome shortly after virus infection and may promote DNA import. In this study, we evaluated whether protein VII uses specific receptor-mediated mechanisms for import into the nucleus. We found that it contains potent nuclear localization signal (NLS) activity by transfection of cultured cells with protein VII fusion constructs and by microinjection of cells with recombinant protein VII fusions. We identified three NLS-containing regions in protein VII by deletion mapping and determined important NLS residues by site-specific mutagenesis. We found that recombinant protein VII and its NLS-containing domains strongly and specifically bind to importin alpha, importin beta, importin 7, and transportin, which are among the most abundant cellular nuclear import receptors. Moreover, these receptors can mediate the nuclear import of protein VII fusions in vitro in permeabilized cells. Considered together, these data support the hypothesis that protein VII is a major NLS-containing adaptor for receptor-mediated import of adenovirus DNA and that multiple import pathways are utilized to promote efficient nuclear entry of the viral genome.

  11. 15 CFR Appendix Vii to Subpart P... - Areas To Be Avoided Boundary Coordinates

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Areas To Be Avoided Boundary Coordinates VII Appendix VII to Subpart P of Part 922 Commerce and Foreign Trade Regulations Relating to... Avoided Boundary Coordinates In The Vicinity of the Florida Keys Point Latitude Longitude 1 25°45.00′N...

  12. 20 CFR 211.12 - Compensation credited for title VII benefits.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Compensation credited for title VII benefits... RETIREMENT ACT CREDITABLE RAILROAD COMPENSATION § 211.12 Compensation credited for title VII benefits... as compensation only for the month in which the employee first filed an application for benefits...

  13. Evaluación inmunoenzimática del antígeno recombinante SAPA en perros infectados naturalmente por Trypanosoma cruzi Immunoenzymatic evaluation of the recombinant SAPA protein of Trypanosoma cruzi in naturally infected dogs

    Directory of Open Access Journals (Sweden)

    Rubén O. Cimino

    2012-09-01

    Full Text Available En este trabajo evaluamos el antígeno recombinante SAPA (Shed Acute Phase Antigen para la detección de anticuerpos anti-Trypanosoma cruzi en sueros de perros infectados naturalmente. La técnica utilizada fue el ELISA y los antígenos utilizados fueron un homogenato de parásitos de T. cruzi (ELISA-H y el recombinante SAPA (ELISA-SAPA. Se analizaron 93 sueros de perros por ELISA-H y ELISA-SAPA, los que fueron agrupados de la siguiente manera: grupo 1 (G1, 11 sueros controles negativos de la ciudad de Salta; grupo 2 (G2, 11 sueros controles positivos, pertenecientes a perros infectados naturalmente con T. cruzi; y grupo 3 (G3, 71 sueros de perros pertenecientes a zona endémica de infección chagásica. La sensibilidad y la especificidad del ELISA-SAPA fueron del 100 %. El índice kappa entre ELISA-H y ELISA-SAPA fue de 0,85. Estos resultados confirman que es adecuado el uso del antígeno recombinante SAPA para el diagnóstico de la infección por T. cruzi en perros.We evaluated the recombinant antigen SAPA (Shed Acute Phase Antigen for the detection of Trypanosoma cruzi antibodies in sera from naturally infected dogs. The technique used was ELISA and the antigens were a homogenate of parasite T. cruzi (ELISA-H and the recombinant SAPA (ELISA-SAPA. We analyzed 93 sera from dogs by ELISA-H and ELISA-SAPA, which were grouped as follows: G1: 11 negative control sera from the city of Salta, G2: 11 positive control sera from dogs naturally infected with T. cruzi and G3: 71 samples of dogs belonging to a Chagas disease-endemic area. The sensitivity and specificity of ELISA-SAPA were 100 %. The kappa index between ELISA-H and ELISA-SAPA was 0,85. These results confirm the use of SAPA antigen in the diagnosis of infection with T. cruzi in dogs.

  14. Produção de antissoro policlonal utilizando a proteína capsidial recombinante do Rupestris stem pitting-associated virus Production of polyclonal antiserum using recombinant coat protein of Rupestris stem pitting -associated virus

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    Marcos Fernando Basso

    2010-11-01

    Full Text Available O Rupestris stem pitting-associated virus (RSPaV é o agente causal das caneluras do lenho da videira. Este trabalho teve como objetivo produzir antissoro policlonal a partir da proteína capsidial (CP recombinante do RSPaV e avaliar a sua especificidade e sensibilidade. O gene da CP do RSPaV, com 780pb, foi previamente caracterizado. Esse gene foi subclonado no sítio de restrição EcoRI, no vetor de expressão pRSET-B e o plasmídeo recombinante foi utilizado para induzir a expressão da CP em Escherichia coli. A CP, ligada a uma cauda de seis histidinas, foi purificada por meio de cromatografia de afinidade em coluna de Ni-NTA a partir do extrato de proteínas totais extraídas de E. coli. A identidade da proteína purificada foi confirmada em SDS-PAGE e Western blot, utilizando-se anticorpos comerciais contra a cauda de seis histidinas. A CP recombinante expressada in vitro apresentou massa molecular de cerca de 31kDa. A proteína purificada foi quantificada e 2,55mg foram utilizados para a imunização de um coelho. O antissoro policlonal obtido reagiu com diferentes isolados deste vírus, extraídos de videiras em ELISA indireto.RSPaV is the causal agent of pitting in the grapevine woody cylinder. The aim of this research was to produce polyclonal antiserum against recombinant RSPaV coat protein (CP and evaluate its specificity and sensibility. The CP gene (780bp of RSPaV was previously characterized. This gene was subcloned into the EcoRI site of the pRSET-B expression vector and the recombinant plasmid was used to induce the expression of the CP in E. coli cells. The CP, fused to a 6-His-tag, was purified from E. coli total protein extract by affinity chromatography using Ni-NTA resin. Identity of the purified protein was confirmed by SDS-PAGE and Western blot, using antibodies against the histidine tail. The in vitro-expressed recombinant CP presented a MW of ca. 31kDa. The purified protein was quantified and 2.55mg used for the

  15. Evaluación serológica de una proteína recombinante a partir de una cepa aislada del virus de la fiebre amarilla en el Perú: un estudio piloto

    Directory of Open Access Journals (Sweden)

    Carlos Yábar V

    2003-10-01

    Full Text Available Objetivo: Evaluar serológicamente una proteína recombinante de 66 kDa (Er66 de una cepa del virus de la fiebre amarilla aislada en el Perú usando anticuerpos inmunoreactivos. Material y métodos: La proteína Er66 fue expresada in vitro en la bacteria Escherichia coli y enfrentada a títulos de 1/100, 1/50 y 1/25 de anticuerpos monoclonales, y a sueros con anticuerpos IgM e IgG inmunoreactivos contra el virus de la fiebre amarilla mediante ensayos de Western blot (WB. Asimismo, se evaluaron proteínas totales de las cepas de referencia del virus dengue (DEN y fiebre amarilla como controles de antigenicidad. Resultados: La proteína recombinante Er66 presentó antigenicidad contra títulos de 1/50 y 1/25 de anticuerpo monoclonal (MAB8701; sin embargo, no se observó inmunoreactividad en sueros positivos para fiebre amarilla y dengue. Los ensayos con extractos crudos de la cepa de fiebre amarilla Asibi 17D (PTFA revelaron que la antigenicidad de las proteínas virales comprendidas entre 60 y 80 kDa fue afectada negativamente a temperaturas desnaturalizantes de 100°C. En cambio, tratamientos con ß-mercaptoetanol sin calor generó un aumento de la antigenicidad de dichas proteínas. Siguiendo este mismo principio, la proteína Er66 fue sometida a tratamientos desnaturalizantes con ß-mercaptoetanol sin incluir calor y fue enfrentada nuevamente a los sueros reactivos. El resultado final reveló que la proteína Er66 generó inmunoreactividad frente a sueros con altos títulos de anticuerpos IgM e IgG para fiebre amarilla mientras que en otras muestras se observó una débil señal. Conclusiones: Los datos obtenidos en este trabajo demuestran que la Er66 pudo ser reconocida por anticuerpos IgM e IgG específicos para fiebre amarilla presentes en sueros de pacientes infectados. Los datos presentados en este artículo sugieren el uso de proteínas recombinantes como posibles candidatas de valor diagnóstico para la FA.

  16. Quality of Activities of General Medicine Subjects VII Teachers in the Different Scenarios Calidad de las actividades de la asignatura medicina general integral VII en los diferentes escenarios docentes

    Directory of Open Access Journals (Sweden)

    Maricel Felicia Castellanos González

    2012-06-01

    Full Text Available Background: as part of the educational process developed in consultation rooms or in any other setting, previously taught contents should be taken into account in order to attain an integration of theoretical knowledge and clinical practice. Objective: to assess teaching settings according to the quality of the activities developed in the subject General Medicine VII, in the context of University Polyclinics. Methods: we conducted a quantitative and qualitative research as to compliance with quality indicators by applying an observation guide in teaching settings of the subject General Medicine VII in the University Polyclinics. Teaching settings evaluated included consultation rooms, vaccination areas, kindergardens, maternity homes, and family planning consultation rooms. Results: consultation rooms, vaccination areas, family planning consultation rooms, schools and kindergardens were assessed as medium proficiency. Only maternity homes and emergency rooms obtained a satisfactory category. Teaching activities with the greatest difficulties were: epidemiological surveillance for the investigation of risk factors, control of communicable disease outbreak, and presentation of the analysis of general health condition of the community. Conclusions: the educational setting was generally assessed as medium proficiency in the subject General Medicine VII. This was caused by the fact that consultation rooms, vaccination areas, family planning consultation rooms, schools and kindergardens were assessed as medium proficiency while only maternity homes and emergency rooms obtained a satisfactory category.Fundamento: en el proceso de enseñanza-aprendizaje que se desarrolla en los diferentes escenarios docentes, se deben tener en cuenta los contenidos ya impartidos, para logar una integración entre los conocimientos teóricos con la práctica m

  17. Observations on the nucleation of ice VII in compressed water

    Science.gov (United States)

    Stafford, Samuel J. P.; Chapman, David J.; Bland, Simon N.; Eakins, Daniel E.

    2017-01-01

    Water can freeze upon multiple shock compression, but the window material determines the pressure of the phase transition. Several plate impact experiments were conducted with liquid targets on a single-stage gas gun, diagnosed simultaneously using photonic doppler velocimetry (PDV) and high speed imaging through the water. The experiments investigated why silica windows instigate freezing above 2.5 GPa whilst sapphire windows do not until 7 GPa. We find that the nucleation of ice occurs on the surfaces of windows and can be affected by the surface coating suggesting the surface energy of fused silica, likely due to hydroxyl groups, encourages nucleation of ice VII crystallites. Aluminium coatings prevent nucleation and sapphire surfaces do not nucleate until approximately 6.5 GPa. This is believed to be the threshold pressure for the homogeneous nucleation of water.

  18. Obtención y evaluación preliminar de un virus canarypox recombinante como candidato a vacuna antirrábica Development and preliminary assessment of a recombinant canarypox virus as an antirabic vaccine candidate

    Directory of Open Access Journals (Sweden)

    Flavia Zanetti

    2012-06-01

    Full Text Available En la Argentina, la rabia está circunscripta a algunas provincias del norte. La disponibilidad de nuevas vacunas que eliminen la manipulación del virus rábico y que permitan el control de la enfermedad es de importancia estratégica nacional y regional. Las vacunas basadas en poxvirus recombinantes se han utilizado con éxito como vacunas antirrábicas a nivel mundial. SI bien estos sistemas no están disponibles comercialmente, la plataforma de obtención de virus canarypox (CNPV recombinantes ya ha sido implementada en nuestro laboratorio. El objetivo de este trabajo fue obtener y evaluar un candidato a vacuna antirrábica basado en CNPV recombinantes que expresan la glicoproteína G (RG del virus rábico (RV. Se construyó un virus recombinante que expresa la secuencia codificante de RG (CNPV-RG. La inoculación de ratones con este virus indujo altos títulos de anticuerpos seroneutralizantes de RV (3,58 y 9,76 Ul/ml después de una o dos inmunizaciones, respectivamente y protegió al 78 % de los animales desafiados intracerebralmente con RV. Además, se determinó que el CNPV-RG posee una potencia relativa de 3,5 Ul/ml. Los resultados obtenidos constituyen la primera etapa en la evaluación del CNPV-RG como candidato a vacuna antirrábica. Se requerirán nuevos ensayos para confirmar su utilidad en especies de interés veterinario.In Argentina, rabies is limited to some northern provinces. Availability of new vaccines abolishing the handling of the rabies virus and allowing disease control has regional and national strategic importance. Vaccines based on recombinant poxviruses have been successfully used as antirabic vaccines worldwide. Although these systems are not commercially available, the platform to obtain recombinant canarypox viruses (CNPV has been previously set up in our laboratory. The aim of this work was the development and evaluation of an antirabic vaccine candidate based on recombinant CNPV expressing the rabies virus (RV

  19. 99Tc(VII) Retardation, Reduction, and Redox Rate Scaling in Naturally Reduced Sediments

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Yuanyuan; Liu, Chongxuan; Kukkadapu, Ravi K.; McKinley, James P.; Zachara, John M.; Plymale, Andrew E.; Miller, Micah D.; Varga, Tamas; Resch, Charles T.

    2015-10-15

    Abstract: An experimental and modeling study was conducted to investigate pertechnetate (Tc(VII)) retardation, reduction, and rate scaling in three sediments from Ringold formation at U.S. Department of Energy’s Hanford site, where 99Tc is a major contaminant in groundwater. Tc(VII) was reduced in all the sediments in both batch reactors and diffusion columns, with a faster rate in a sediment containing a higher concentration of HCl-extractable Fe(II). Tc(VII) migration in the diffusion columns was reductively retarded with retardation degrees correlated with Tc(VII) reduction rates. The reduction rates were faster in the diffusion columns than those in the batch reactors, apparently influenced by the spatial distribution of redox-reactive minerals along transport paths that supplied Tc(VII). X-ray computed tomography and autoradiography were performed to identify the spatial locations of Tc(VII) reduction and transport paths in the sediments, and results generally confirmed the newly found behavior of reaction rate changes from batch to column. The results from this study implied that Tc(VII) migration can be reductively retarded at Hanford site with a retardation degree dependent on reactive Fe(II) content and its distribution in sediments. This study also demonstrated that an effective reaction rate may be faster in transport systems than that in well-mixed reactors.

  20. The effect of neonatal gene therapy on skeletal manifestations in mucopolysaccharidosis VII dogs after a decade.

    Science.gov (United States)

    Xing, Elizabeth M; Knox, Van W; O'Donnell, Patricia A; Sikura, Tracey; Liu, Yuli; Wu, Susan; Casal, Margret L; Haskins, Mark E; Ponder, Katherine P

    2013-06-01

    Mucopolysaccharidosis (MPS) VII is a lysosomal storage disease due to deficient activity of β-glucuronidase (GUSB), and results in glycosaminoglycan accumulation. Skeletal manifestations include bone dysplasia, degenerative joint disease, and growth retardation. One gene therapy approach for MPS VII involves neonatal intravenous injection of a gamma retroviral vector expressing GUSB, which results in stable expression in liver and secretion of enzyme into blood at levels predicted to be similar or higher to enzyme replacement therapy. The goal of this study was to evaluate the long-term effect of neonatal gene therapy on skeletal manifestations in MPS VII dogs. Treated MPS VII dogs could walk throughout their lives, while untreated MPS VII dogs could not stand beyond 6 months and were dead by 2 years. Luxation of the coxofemoral joint and the patella, dysplasia of the acetabulum and supracondylar ridge, deep erosions of the distal femur, and synovial hyperplasia were reduced, and the quality of articular bone was improved in treated dogs at 6 to 11 years of age compared with untreated MPS VII dogs at 2 years or less. However, treated dogs continued to have osteophyte formation, cartilage abnormalities, and an abnormal gait. Enzyme activity was found near synovial blood vessels, and there was 2% as much GUSB activity in synovial fluid as in serum. We conclude that neonatal gene therapy reduces skeletal abnormalities in MPS VII dogs, but clinically-relevant abnormalities remain. Enzyme replacement therapy will probably have similar limitations long-term.

  1. Producción de anticuerpos policlonales IgG contra la proteina iduronato-2-sulfato sulfatasa y desarrollo de un sistema de detección para IDS humana recombinante.

    Directory of Open Access Journals (Sweden)

    Olga Peña

    2005-06-01

    Full Text Available Introducción. La enfermedad de Hunter es un trastorno lisosómico caracterizado por la deficiencia de la enzima iduronato-2-sulfato sulfatasa (IDS (EC 3.1.6.13. Esta enfermedad, al igual que muchos trastornos metabólicos, son patologías intratables mediante la terapéutica convencional; sin embargo, existe la posibilidad de ser tratada alternativamente mediante terapia génica o terapia de reemplazo enzimático. Objetivo. El Instituto de Errores Innatos del Metabolismo (IEIM ha desarrollado un sistema de expresión de sulfatasas para producir IDS humana recombinante (IDShr en Escherichia coli y Pichia pastoris, con resultados favorables. El objetivo principal de este trabajo fue desarrollar un sistema de detección de IDS humana recombinante. Materiales y métodos. Para el efecto, se inmunizaron con IDS comercial de TKT (Cambridge, MA dos conejos de raza Nueva Zelanda blanca y los anticuerpos purificados a partir del suero se utilizaron en el desarrollo de una técnica semicuantitativa por dot-blot. Diferentes muestras de extractos crudos de fermentaciones con P. pastoris y E. coli se procesaron con el fin de poder determinar la presencia de la enzima. Resultados. Se demostró que los anticuerpos eran específicos en el reconocimiento de la IDS sin presentar reactividad cruzada con proteínas contaminantes de los extractos crudos. Conclusión. Por consiguiente, los anticuerpos se podrán usar en el desarrollo de una técnica ELISA tipo sandwich como método de detección y cuantificación de la enzima y en procesos de purificación de la misma mediante cromatografía de afinidad.

  2. Construcción y expresión de una proteína recombinante de la envoltura del virus linfotrópico humano tipo I (HTLV-I.

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    Paula Ordóñez

    2009-11-01

    Full Text Available El virus linfotrópico humano tipo I de las células T (HTLV-I es un oncorretrovirus asociado con la leucemia de células T adultas (ATL y la Paraparesia Espástica Tropical (PET. El gen env del virus codifica la glicoproteína precursora gp62, que mediante procesamiento produce la gp21 (transmembranal y la gp46 (superficie. Se ha demostrado la alta inmunorreactividad de la gp46 en sueros de individuos infectados. Con el propósito de obtener una proteína recombinante útil en diagnóstico y en una potencial vacuna, se clonó en el vector de expresión de Baculovirus “bácmido” pBlueBac4.5His el segmento de 144bp obtenido mediante reacción en cadena de la polimerasa (PCR con cebadores que amplifican la región 5622-5765 nt. El construido pBlueBacRENV fue transfectado en células del insecto Mamestrae brassicae (clon MB 8003 recuperándose la proteína recombinante. La proteína RENV reaccionó específicamente con suero de personas infectadas con HTLV-I. Los resultados obtenidos mostraron que la proteína RENV puede ser utilizada para el diagnóstico serológico de la infección por HTLV-I y en la evaluación de nuevas vacunas.

  3. Clinical and Epidemiological behavior of Acute Dentoalveolar Abscess in Patients of the Health Area VII in Cienfuegos

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    Ana Belkys Hernández Millán

    2015-02-01

    Full Text Available Background: acute dentoalveolar abscess is a dental emergency and one of the major condition affecting the population; however, there are few studies on the subject. Objective: to describe the clinical and epidemiological characteristics of acute dentoalveolar abscess in patients of the health area VII in Cienfuegos. Methods: a descriptive study was conducted from January to December 2013 in the Health Area VII, Cienfuegos. The universe consisted of 672 patients and the sample included 374 individuals selected by simple random sampling. Primary data recording and data collection was obtained from medical records prior informed consent of the patients. The main variables were age, sex, pulp irritating agents. Results: females were more affected with 55, 35% and the 19-34 year age group with 33.69%. The main pulp irritator was microbial, 59.36%. Among the iatrogenic factors, the remains of decayed tissues were significant with 32.35%. Conclusions: there is a high number of patients with acute dentoalveolar abscess, thus, as a dental emergency, the dentist should know the characteristics and factors that develop the disease to promote a comprehensive job in terms of health promotion, prevention, treatment and rehabilitation of affected patients.

  4. Perfil hormonal sexual de mulheres em programa crônico de diálise em tratamento com eritropoetina humana recombinante Sex hormones in women on dialysis

    Directory of Open Access Journals (Sweden)

    Rosina Conceição Graçaplena Dal Maso

    2003-01-01

    Full Text Available A IRC (insuficiência renal crônica terminal é acompanhada por alterações sistêmicas, inclusive do eixo hipotálamo-hipofisário-gonadal. Após o aparecimento da EPO (eritropoetina humana recombinante, surgiram relatos de melhora da disfunção sexual em homens, havendo relativa escassez de dados sobre dosagens hormonais e função sexual das mulheres. No Brasil, há poucos dados sobre o perfil hormonal de mulheres em programa crônico de diálise. OBJETIVOS: Avaliação e comparação do perfil hormonal sexual, através das dosagens de FSH (hormônio folículo estimulante, LH (hormônio luteinizante, PRL (prolactina e E2 (estradiol de mulheres em programa crônico de diálise, sob terapêutica com EPO. MÉTODOS: Foram avaliadas 47 mulheres (uma em diálise peritoneal intermitente, quatro em diálise peritoneal ambulatorial contínua e 42 em hemodiálise, subdivididas em três grupos: (1 Grupo I, de mulheres com menos de 48 anos de idade e menstruando regularmente; (2 Grupo II, de mulheres com menos de 48 anos de idade e amenorreicas; (3 Grupo III, de mulheres com mais de 48 anos de idade e amenorreicas. Os grupos foram comparados entre si através do teste de Mann-Whitney para duas amostras independentes. RESULTADOS: Todos os grupos mostraram valores normais das dosagens séricas de FSH, LH e E2, e não houve diferença estatisticamente significante nos níveis desses hormônios entre as mulheres que menstruam e as amenorreicas com idade inferior a 48 anos. Os níveis de PRL encontravam-se acima da normalidade em todos os grupos, independentemente da idade e do padrão menstrual, não havendo diferenças estatísticas significantes entre os grupos. As pacientes do grupo III apresentaram perfis hormonais compatíveis com a menopausa, ou seja, níveis séricos de FSH e LH elevados e de E2 diminuídos.OBJECTIVE: The end stage renal disease has accompaniments in body systems, including hormonal changes. The present study was designed to

  5. Expresión y purificación de las proteínas recombinantes estructurales VP5, VP6 y VP8 del rotavirus / Expression and purification of recombinant structural rotavirus proteins VP5, VP6 and VP8

    OpenAIRE

    Moreno Rueda, Luz Yurany

    2010-01-01

    La identificación de posibles receptores del rotavirus y su participación durante la unión y penetración del virus en células suceptibles a la infección han requerido la expresión y purificación de las proteínas virales. Por tanto, el objetivo general de este trabajo consistió en la expresión de las proteínas estructurales VP5 y VP8 del rotavirus en un sistema bacteriano y VP6 en células MA104 mediante la infección con el virus vaccinia recombinante. Las proteínas recombinantes fueron purific...

  6. ICSBEP Criticality Benchmark Eigenvalues with ENDF/B-VII.1 Cross Sections

    Energy Technology Data Exchange (ETDEWEB)

    Kahler, Albert C. III [Los Alamos National Laboratory; MacFarlane, Robert [Los Alamos National Laboratory

    2012-06-28

    We review MCNP eigenvalue calculations from a suite of International Criticality Safety Benchmark Evaluation Project (ICSBEP) Handbook evaluations with the recently distributed ENDF/B-VII.1 cross section library.

  7. Design and engineering aspects of the main components for the Wendelstein VII-AS stellarator experiment

    Energy Technology Data Exchange (ETDEWEB)

    Mathis, R.; Sapper, J. (Max-Planck-Institut fuer Plasmaphysik, Garching (Germany, F.R.))

    1990-02-01

    In this paper the design of the essential components of the newly constructed Wendelstein VII-AS stellarator experiment at Garching are described, and the first technical and experimental results are reported. (orig.).

  8. Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: Efficacy of vaccination

    Science.gov (United States)

    Here we describe the characterization a virulent genotype VII Newcastle disease virus (NDV) from Venezuela and evaluate the efficacy of heterologous genotype commercial vaccination under field and controlled rearing conditions. Biological pathotyping and molecular analysis were applied. Results sh...

  9. The Trigeminal (V) and Facial (VII) Cranial Nerves: Head and Face Sensation and Movement

    OpenAIRE

    Sanders, Richard D.

    2010-01-01

    There are close functional and anatomical relationships between cranial nerves V and VII in both their sensory and motor divisions. Sensation on the face is innervated by the trigeminal nerves (V) as are the muscles of mastication, but the muscles of facial expression are innervated mainly by the facial nerve (VII) as is the sensation of taste. This article briefly reviews the anatomy of these cranial nerves, disorders of these nerves that are of particular importance to psychiatry, and some ...

  10. Onset of ice VII phase during ps laser pulse propagation through liquid water

    Science.gov (United States)

    Kumar, V. Rakesh; Kiran, P. Prem

    2017-01-01

    Water dominantly present in liquid state on earth gets transformed to crystalline polymorphs under different dynamic loading conditions. Out of different crystalline phases discovered till date, ice VII is observed to be stable over wide pressure (2-63 GPa) and temperature (>273 K) ranges. The formation of ice VII crystalline structure has been vastly reported during high pressure static compression using diamond anvil cell and propagation of high energy (>50 mJ/pulse) nanosecond laser pulse induced dynamic high pressures through liquid water. We present the onset of ice VII phase at low threshold of 2 mJ/pulse during 30 ps (532 nm, 10 Hz) laser pulse induced shock propagating through liquid water. Role of input pulse energy on the evolution of Stoke's and anti-Stoke's Raman shift of the dominant A1g mode of ice VII, filamentation, free-electrons, plasma shielding is presented. The H-bond network rearrangement, electron ion energy transfer time coinciding with the excitation pulse duration supported by the filamentation and plasma shielding of the ps laser pulses reduced the threshold of ice VII structure formation. Filamentation and the plasma shielding have shown the localized creation and sustenance of ice VII structure in liquid water over 3 mm length and 50 μm area of cross-section.

  11. Energy levels and radiative rates for transitions in Ti VII

    CERN Document Server

    Aggarwal, KM

    2013-01-01

    We report calculations of energy levels, radiative rates, oscillator strengths and line strengths for transitions among the lowest 231 levels of Ti VII. The general-purpose relativistic atomic structure package ({\\sc grasp}) and flexible atomic code ({\\sc fac}) are adopted for the calculations. Radiative rates, oscillator strengths and line strengths are provided for all electric dipole (E1), magnetic dipole (M1), electric quadrupole (E2) and magnetic quadrupole (M2) transitions among the 231 levels, although calculations have been performed for a much larger number of levels (159,162). In addition, lifetimes for all 231 levels are listed. Comparisons are made with existing results and the accuracy of the data is assessed. In particular, the most recent calculations reported by Singh {\\em et al} [Can J. Phys. {\\bf 90} (2012) 833] are found to be unreliable, with discrepancies for energy levels of up to 1 Ryd and for radiative rates of up to five orders of magnitude for several transitions, particularly the we...

  12. Collagen VII plays a dual role in wound healing.

    Science.gov (United States)

    Nyström, Alexander; Velati, Daniela; Mittapalli, Venugopal R; Fritsch, Anja; Kern, Johannes S; Bruckner-Tuderman, Leena

    2013-08-01

    Although a host of intracellular signals is known to contribute to wound healing, the role of the cell microenvironment in tissue repair remains elusive. Here we employed 2 different mouse models of genetic skin fragility to assess the role of the basement membrane protein collagen VII (COL7A1) in wound healing. COL7A1 secures the attachment of the epidermis to the dermis, and its mutations cause a human skin fragility disorder coined recessive dystrophic epidermolysis bullosa (RDEB) that is associated with a constant wound burden. We show that COL7A1 is instrumental for skin wound closure by 2 interconnected mechanisms. First, COL7A1 was required for re-epithelialization through organization of laminin-332 at the dermal-epidermal junction. Its loss perturbs laminin-332 organization during wound healing, which in turn abrogates strictly polarized expression of integrin α6β4 in basal keratinocytes and negatively impacts the laminin-332/integrin α6β4 signaling axis guiding keratinocyte migration. Second, COL7A1 supported dermal fibroblast migration and regulates their cytokine production in the granulation tissue. These findings, which were validated in human wounds, identify COL7A1 as a critical player in physiological wound healing in humans and mice and may facilitate development of therapeutic strategies not only for RDEB, but also for other chronic wounds.

  13. Burn wound angiogenesis is increased by exogenously administered recombinant leptin in rats A administração exógena de leptina recombinante induz à angiogênese em queimaduras cutâneas provocadas em ratos

    Directory of Open Access Journals (Sweden)

    Ioannis Liapakis

    2008-04-01

    Full Text Available BACKGROUND: Leptin is a potent direct angiogenic factor that stimulates endothelial cell migration and activation in vitro and angiogenesis in vivo. In addition, leptin has been discussed to play an important role in angiogenesis, as it promotes the formation of new blood vessels. PURPOSE: The effect of exogenously administered leptin on the healing process of a full tissue burn wound model. METHODS: Sixty-three Sprague-Dawley male rats were used. Full tissue burn wound was created by electrocautery. The width of the pin was 0.3 cm; its length was 2 cm and was used at the "cut" modulation. Rats were divided into seven groups of nine animals each. Burn wounds were injected with murine recombinant leptin and the rats were sacrificed 3, 7 and 9 days after surgery. Every group had obtained three animals for the three different days of sacrifice. Three different leptin doses of 250 pg/ml, 500 pg/ml and 1000 pg/ml were used in different animal groups (A, B and C. For every one of the three leptin doses used, another animal group was evaluated by using the combined injection of leptin and antileptin (A1, B1, and C1, in order to study the inhibitory effect to the leptin factor. Nine rats were served as controls. These were injected with 0.3 ml water for injection solution and sacrificed at the same time intervals. After sacrifice of the animals, the skin was grossly determined by its appearance, colour and texture. Full thickness burn wounds were dissected for histological examination. A qualitative analysis of angiogenesis in the burn wound was conducted following a standard hematoxylin and eosin stain. The wound tissue samples from each experimental group underwent immunohistochemical evaluation of microvessel density by endothelial cell staining with mouse anti-rat CD 34 monoclonal antibody. RESULTS: The most impressive growth of new blood vessels appeared seven and nine days after treatment with the highest leptin doses. There were no significant

  14. Technetium (VII) fixation in synthetic oxides under aerobic conditions; Fijacion de tecnecio (VII) en oxidos sinteticos en condiciones aerobicas

    Energy Technology Data Exchange (ETDEWEB)

    Arizpe M, L. G. [Instituto Tecnologico de Toluca, Av. Tecnologico s/n, Fraccionamiento La Virgen, 52149 Metepec, Estado de Mexico (Mexico); Badillo A, V. E., E-mail: letygame_06@hotmail.com [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)

    2012-10-15

    The retention of fission products as the technetium in different materials to observe the behavior of anionic species, present in the spent fuel, is of vital importance in the evaluation of the safety of a radioactive waste storage. The radionuclide {sup 99}Tc is one of the fission products of long half life of 2.11 X 10{sup 5} years, which could be liberated in the underground waters that surround to a geologic warehouse of high level wastes, in the chemical form TcO{sub 4}{sup -}, which prevails in aerated solutions since is extremely soluble. In this work was evaluated the use of synthetic oxides as potential adsorbents of the radioactive technetium and to study the importance of the superficial properties of these solids in the radionuclide migration. Two synthetic oxides were studied, the titanium and aluminium oxide. The results demonstrate that the retention of Tc (VII) under aerobic conditions of the solution is worthless in the interval of studied ph of 4 at 9 for both solids; however when the ph of the solution diminishes to ph values of 3, important kD are obtained in the case of the titanium oxide, what shows the importance of the surface properties of these oxides to interpret the radionuclide retention. (Author)

  15. In silico dissection of Type VII Secretion System components across bacteria: New directions towards functional characterization

    Indian Academy of Sciences (India)

    Chandrani Das; Tarini Shankar Ghosh; Sharmila S Mande

    2016-03-01

    Type VII Secretion System (T7SS) is one of the factors involved in virulence of Mycobacteriun tuberculosis H37Rv. Numerous research efforts have been made in the last decade towards characterizing the components of this secretion system. An extensive genome-wide analysis through compilation of isolated information is required to obtain a global view of diverse characteristics and pathogenicity-related aspects of this machinery. The present study suggests that differences in structural components (of T7SS) between Actinobacteria and Firmicutes, observed earlier in a few organisms, is indeed a global trend. A few hitherto uncharacterized T7SS-like clusters have been identified in the pathogenic bacteria Enterococcus faecalis, Saccharomonospora viridis, Streptococcus equi, Streptococcuss gordonii and Streptococcus sanguinis. Experimental verification of these clusters can shed lights on their role in bacterial pathogenesis. Similarly, verification of the identified variants of T7SS clusters consisting additional membrane components may help in unraveling new mechanism of protein translocation through T7SS. A database of various components of T7SS has been developed to facilitate easy access and interpretation of T7SS related data.

  16. Intensive Farmaco- Surveillance of Interferon Alpha 2b Recombinant in Multiple Sclerosis. Farmacovigilancia intensiva al interferón alfa 2b recombinante en la esclerosis múltiple.

    Directory of Open Access Journals (Sweden)

    Hailen Bobillo López

    recombinant was safe in the treatment of the multiple sclerosis in these patients.

     

    Fundamento: El interferón alfa 2b recombinante, de producción nacional, se utiliza en el tratamiento de diferentes enfermedades, entre ellas la esclerosis múltiple. Para su comercialización se necesita conocer su margen de seguridad. Objetivo: Evaluar las reacciones adversas del interferón alfa 2b recombinante en el tratamiento de la esclerosis múltiple. Método: Durante el período comprendido entre 1996 y 2006, se revisaron las 70 historias clínicas y cuadernos de recogida de datos de los pacientes incluidos en el ensayo clínico nacional, fase IV aleatorizado, a doble ciegas, desarrollado en la Clínica de Esclerosis Múltiple del Hospital ¨Dr. Gustavo Aldereguía Lima¨ de Cienfuegos. Con respecto al total de reacciones adversas manifestadas, se analizó tipo, tiempo de duración, empleo de tratamiento o no para contrarrestarlas, grado de intensidad (leve, moderada, grave o mortal y de causalidad (definitiva, probable, posible, condicional o no relacionada. Resultados: Del total de pacientes, 53 presentaron 207 reacciones adversas al interferón. Las más frecuentes fueron: fiebre, cefalea, escalofríos, artralgia, astenia y mialgia, siendo la mayoría efectos colaterales moderados y de carácter definitivo. En 197 pacientes el desenlace fue favorable. Conclusiones: El uso del interferón alfa 2β recombinante fue seguro en el tratamiento de la esclerosis múltiple en estos pacientes.

  17. Seguridad de la terapia de interferón alfa 2b recombinante más ribavirina en la hepatitis crónica C Safety of recombinant interferon alpha 2b plus ribavirin in chronic hepatitis C

    Directory of Open Access Journals (Sweden)

    Yoan Antonio Sánchez Rodríguez

    2011-03-01

    Full Text Available INTRODUCCIÓN: la hepatitis crónica C ha adquirido rango de pandemia. El virus de la hepatitis C se ha convertido en la causa principal de hepatitis crónica, cirrosis hepática, hepatocarcinoma, y trasplante de hígado a nivel mundial. OBJETIVO: identificar los efectos adversos asociados a la terapia combinada interferón alfa 2b recombinante más ribavirina durante la evolución del tratamiento y hasta 8 semanas después de finalizado, así como los principales efectos asociados a salidas temporales o definitivas de esta terapia. MÉTODOS: estudio de farmacovigilancia cuya serie estuvo conformada por 122 pacientes con hepatitis crónica C atendidos en el Instituto de Gastroenterología desde mayo de 2001 hasta mayo de 2006. Se utilizó interferón alfa 2b recombinante (3 millones de unidades 3 veces por semana más ribavirina (1 000 o 1 200 mg diarios en dependencia del peso corporal durante 48 semanas. RESULTADOS: el 88,5 % del total de casos presentó efectos adversos; de ellos el 79,5 % correspondió al síndrome seudogripal, seguido de manifestaciones hematológicas, neuropsiquiátricas, gastrointestinales, entre otras menos frecuentes. El 6,6 % de la serie presentó salidas temporales del tratamiento por efecto adverso distinto de la anemia y 4 pacientes, salidas definitivas del estudio, tres por anemia hemolítica severa y uno con hipertiroidismo no controlable. CONCLUSIONES: la terapia combinada interferón alfa 2b recombinante más ribavirina resulta segura, donde el mayor número de casos presentó síndrome seudogripal como efecto adverso más frecuente. Las manifestaciones hematológicas asociadas a las salidas definitivas del estudio permitieron recomendar seguimiento estricto de la hemoglobina y profundizar en el diagnóstico y tratamiento de los principales efectos adversos presentes en otros sistemas y asociados a esta terapia.INTRODUCTION: chronic hepatitis C has reached the category of pandemic. The hepatitis C virus is the

  18. Immunogenicity and antigenicity of the recombinant EMA-1 protein of Theileria equi expressed in the yeast Pichia pastoris Imunogenicidade e antigenicidade da proteína recombinante EMA-1 de Theileria equi expressa em Pichia pastoris

    Directory of Open Access Journals (Sweden)

    Leandro Q. Nizoli

    2009-06-01

    Full Text Available The equine piroplasmosis caused by Theileria equi is one of the most important parasitic diseases of the equine, causing damage to animal health and economic losses. In T. equi, 2 merozoite surface proteins, equi merozoite antigen EMA-1 and EMA-2, have been identified as the most immunodominant antigens. This suggests that these antigens might be used as immunobiological tools. The EMA-1 of Theileria equi was cloned and expressed in the yeast Pichia pastoris. The transformed yeast was grown at high cell density, expressing up to 389 mg.L-1 of recombinant protein. The protein was concentrated and detected in Dot blot. The recombinant product was antigenically similar to the native protein as determined using monoclonal antibodies, and polyclonal antibodies obtained from equines naturally infected with T. equi. The immunogenicity of rEMA-1 protein was demonstrated by IFAT using sera from recombinant-protein-immunized mice using aluminum hydroxide as adjuvant. All animals vaccinated with rEMA-1 developed a high specific antibody response. This results suggest that rEMA-1expressed in P. pastoris might be a strong candidate to be used as an antigen for immune diagnostics as well as a vaccine antigen.A piroplasmose equina causada por Theileria equi é uma das mais importantes doenças parasitárias de equídeos, causando danos a saúde animal e perdas econômicas. Em T. equi, 2 proteínas de superfície de merozoítos, equi merozoite antigen EMA-1 e EMA-2, têm sido identificadas como antígenos imunodominantes. Sugerindo que estes antígenos possam ser usados como produtos imunobiológicos. O gene EMA-1 de T. equi foi clonado e expressado na levedura Pichia pastoris. As leveduras transformadas foram cultivadas a altas densidades celulares expressando 389 mg.L-1 de proteína recombinante. A proteína foi concentrada e detectada em Dot blot. O produto recombinante foi antigenicamente similar à proteína nativa quando determinado usando anticorpo

  19. Radiographic Evaluation of Bones and Joints in Mucopolysaccharidosis I and VII Dogs After Neonatal Gene Therapy

    Science.gov (United States)

    Herati, Ramin Sedaghat; Knox, Van W.; O’Donnell, Patricia; D’Angelo, Marina; Haskins, Mark E.; Ponder, Katherine P.

    2009-01-01

    Mucopolysaccharidosis I (MPS I) and MPS VII are due to deficient activity of the glycosaminoglycan-degrading lysosomal enzymes α-L-iduronidase and β-glucuronidase, respectively, and result in abnormal bones and joints. Here, the severity of skeletal disease in MPS I and MPS VII dogs and the effects of neonatal gene therapy were evaluated. For untreated MPS VII dogs, the lengths of the second cervical vertebrae (C2) and the femur were only 56% and 84% of normal, respectively, and bone dysplasia and articular erosions, and joint subluxation were severe. Previously, we reported that neonatal intravenous injection of a retroviral vector (RV) with the appropriate gene resulted in expression in liver and blood cells, and high serum enzyme activity. In this study, we demonstrate that C2 and femurs of RV-treated MPS VII dogs were longer at 82% and 101% of normal, respectively, and there were partial improvements of qualitative abnormalities. For untreated MPS I dogs, the lengths of C2 and femurs (91% and 96% of normal, respectively) were not significantly different from normal dogs. Qualitative changes in MPS I bones and joints were generally modest and were partially improved with RV treatment, although cervical spine disease was severe and was difficult to correct with gene therapy in both models. The greater severity of skeletal disease in MPS VII than in MPS I dogs may reflect accumulation of chondroitin sulfate in cartilage in MPS VII, or could relate to the specific mutations. Neonatal RV-mediated gene therapy ameliorates, but does not prevent, skeletal disease in MPS I and MPS VII dogs. PMID:18707908

  20. Desenvolvimento e padronização do Dot-ELISA usando peptídeos recombinantes para o diagnóstico sorológico de Neospora caninum

    Directory of Open Access Journals (Sweden)

    Rafael D. Blanco

    2014-08-01

    Full Text Available A neosporose é reconhecida como uma das maiores causas de aborto e perdas neonatais em bovinos de leite e corte em todo o mundo. Nos últimos anos esta doença tem atraído o interesse de pesquisadores com foco na epidemiologia e métodos eficazes de diagnóstico desta doença. No presente estudo objetivou-se desenvolver e padronizar um teste Dot-ELISA para o diagnóstico sorológico de Neospora caninum com um peptídeo recombinate como antígeno, visando o desenvolvimento de um kit para diagnóstico a campo. O peptídeo recombinante (rNcGRA1 foi desenhado com base na metodologia de genética reversa de epítopos antigênicos originados de uma proteína de grânulos densos de N. caninum, e sintetizado pela GenScript (USA. Produzido mediante o processo fermentativo em leveduras Pichia pastoris KM71. Para a padronização do Dot-ELISA, membranas de nitrocelulose de 0.22µm foram sensibilizadas com 1µL do antígeno e posteriormente os soros foram diluídos em solução de lavagem e incubados durante 1 hora. A revelação foi feita mediante a adição de Proteína G marcada com peroxidase por 30 minutos, seguido da solução reveladora a base de 3,3’-Diaminobenzidine (DAB. Logo após a padronização foram testados 44 soros bovinos diagnosticados por imunofluorescência indireta (RIFI, obtendo-se uma concordância nos resultados do teste de 95,5% e uma sensibilidade e especificidade de 100% e 92% respectivamente. Quanto ao Kit para diagnóstico a campo na Plataforma Tecnológica RapidFlow-Through Miriad®, o peptídeo rNcGRA1 apresentou marcações visíveis ao reagir com os soros positivos, e não apresentou marcações usando os soros negativos. Este estudo é o primeiro a utilizar peptídeos recombinantes e mostrar-se eficiente para o diagnóstico sorológico de bovinos naturalmente infetados por N. caninum.

  1. Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima

    Science.gov (United States)

    Larrea, Andres A.; Pedroso, Ilene M.; Malhotra, Arun; Myers, Richard S.

    2008-01-01

    Exonuclease VII was first identified in 1974 as a DNA exonuclease that did not require any divalent cations for activity. Indeed, Escherichia coli ExoVII was identified in partially purified extracts in the presence of EDTA. ExoVII is comprised of two subunits (XseA and XseB) that are highly conserved and present in most sequenced prokaryotic genomes, but are not seen in eukaryotes. To better understand this exonuclease family, we have characterized an ExoVII homolog from Thermotoga maritima. Thermotoga maritima XseA/B homologs TM1768 and TM1769 were co-expressed and purified, and show robust nuclease activity at 80°C. This activity is magnesium dependent and is inhibited by phosphate ions, which distinguish it from E. coli ExoVII. Nevertheless, both E. coli and T. maritima ExoVII share a similar putative active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. We show that these residues, Asp235 and Asp240, are essential for the nuclease activity of T. maritima ExoVII. We hypothesize that the ExoVII family of nucleases can be sub-divided into two sub-families based on EDTA resistance and that T. maritima ExoVII is the first member of the branch that is characterized by EDTA sensitivity and inhibition by phosphate. PMID:18812402

  2. Reversal of established bone pathology in MPS VII mice following lentiviral-mediated gene therapy.

    Science.gov (United States)

    Derrick-Roberts, Ainslie L K; Panir, Kavita; Pyragius, Carmen E; Zarrinkalam, Krystyna H; Atkins, Gerald J; Byers, Sharon

    2016-11-01

    Severe, progressive skeletal dysplasia is a major symptom of multiple mucopolysaccharidoses (MPS) types. While a gene therapy approach initiated at birth has been shown to prevent the development of bone pathology in different animal models of MPS, the capacity to correct developed bone disease is unknown. In this study, ex vivo micro-computed tomography was used to demonstrate that bone mass and architecture of murine MPS VII L5 vertebrae were within the normal range at 1month of age but by 2months of age were significantly different to normal. The difference between normal and MPS VII BV/TV increased with age reaching a maximal difference at approximately 4months of age. In mature MPS VII bone BV/TV is increased (51.5% versus 21.5% in normal mice) due to an increase in trabecular number (6.2permm versus 3.8permm in normal mice). The total number of osteoclasts in the metaphysis of MPS VII mice was decreased, as was the percentage of osteoclasts attached to bone. MPS VII osteoblasts produced significantly more osteoprotegerin (OPG) than normal osteoblasts and supported the production of fewer osteoclasts from spleen precursor cells than normal osteoblasts in a co-culture system. In contrast, the formation of osteoclasts from MPS VII spleen monocytes was similar to normal in vitro, when exogenous RANKL and m-CSF was added to the culture medium. Administration of murine β-glucuronidase to MPS VII mice at 4months of age, when bone disease was fully manifested, using lentiviral gene delivery resulted in a doubling of osteoclast numbers and a significant increase in attachment capacity (68% versus 29.4% in untreated MPS VII animals). Bone mineral volume rapidly decreased by 39% after gene therapy and fell within the normal range by 6months of age. Collectively, these results indicate that lentiviral-mediated gene therapy is effective in reversing established skeletal pathology in murine MPS VII. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Technetium (VII) Co-precipitation with Framework Aluminosilicates

    Energy Technology Data Exchange (ETDEWEB)

    Harsh, James B. [Washington State Univ., Pullman, WA (United States); Dickson, Johnbull Otah [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Pierce, Eric M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bargar, John [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2015-07-13

    Technetium-99 (99Tc), a long-lived radionuclide, is one of the most widespread contaminants within the Hanford subsurface. At some depths, it is only extractable with strong acids, suggesting incorporation into a solid phase. We hypothesized that Tc may have coprecipitated with feldspathoid aluminosilicates under waste tanks that had leaked caustic solutions into the vadose zone. Our objectives were to determine if Tc could be incorporated into the feldspathoids cancrinite and sodalite and under what conditions coprecipitation could occur. Our hypothesis was that sodalite was more likely to incorporate and retain Tc. Our approach was to use known methods of feldspathoid formation in solutions resembling those in Hanford waste tanks contacting sediments in terms of major ion (Na, NO3, OH, Al(OH)4, and Si(OH)4 concentrations. In some cases, Al and Si were supplied from zeolite. We used perrhenate (ReO4) as a surrogate for pertechnetate (TcO4) to avoid the radioactivity. The major findings of this study were 1) ReO4 could be incorporated into either sodalite or cancrinite but the concentration in the solid was < 1% of the competing ion Cl, NO3, or NO2. 2) The small amount of ReO4 incorporated was not exchangeable with NO3 or NO2. 3) In sodalite, NO3 was highly preferred over ReO4 but significant Re-sodalite was formed when the mole fraction in solution (Re/Re+N) exceeded 0.8. 4) A nonlinear relation between the unit cell parameter and amount of Re incorporated suggested that a separate Re-sodalite phase was formed rather than a solid solution. 5) We determined that sodalite preference for sodalite in the presence of different anions increased with the ionic size of the competing anion: Cl < CO3 < NO3 < SO4 < MnO4 < WO4 and significant incorporation did not occur unless the difference in anion radii was less than 12%. 6) Re(VII) was not significantly reduced to Re(IV) under the conditions of this experiment and Re appeared to be a good surrogate for Tc under oxidizing

  4. Aortic Root Dilatation in Mucopolysaccharidosis I–VII

    Directory of Open Access Journals (Sweden)

    Meena Bolourchi

    2016-11-01

    Full Text Available The prevalence of aortic root dilatation (ARD in mucopolysaccharidosis (MPS is not well documented. We investigated aortic root measurements in 34 MPS patients at the Children’s Hospital of Orange County (CHOC. The diagnosis, treatment status, age, gender, height, weight and aortic root parameters (aortic valve annulus (AVA, sinuses of Valsalva (SoV, and sinotubular junction (STJ were extracted by retrospective chart review and echocardiographic measurements. Descriptive statistics, ANOVA, and paired post-hoc t-tests were used to summarize the aortic dimensions. Exact binomial 95% confidence intervals (CIs were constructed for ARD, defined as a z-score greater than 2 at the SoV. The patient age ranged from 3.4–25.9 years (mean 13.3 ± 6.1, the height from 0.87–1.62 meters (mean 1.24 ± 0.21, and the weight from 14.1–84.5 kg (mean 34.4 ± 18.0. The prevalence of dilation at the AVA was 41% (14/34; 95% CI: 25%–59%; at the SoV was 35% (12/34; 95% CI: 20%–54%; and at the STJ was 30% (9/30; 95% CI: 15%–49%. The highest prevalence of ARD was in MPS IVa (87.5%. There was no significant difference between mean z-scores of MPS patients who received treatment with hematopoietic stem cell transplantation (HSCT or enzyme replacement therapy (ERT vs. untreated MPS patients at the AVA (z = 1.9 ± 2.5 vs. z = 1.5 ± 2.4; p = 0.62, SoV (z = 1.2 ± 1.6 vs. z = 1.3 ± 2.2; p = 0.79, or STJ (z = 1.0 ± 1.8 vs. z = 1.2 ± 1.6; p = 0.83. The prevalence of ARD was 35% in our cohort of MPS I–VII patients. Thus, we recommend screening for ARD on a routine basis in this patient population.

  5. Aortic Root Dilatation in Mucopolysaccharidosis I-VII.

    Science.gov (United States)

    Bolourchi, Meena; Renella, Pierangelo; Wang, Raymond Y

    2016-11-29

    The prevalence of aortic root dilatation (ARD) in mucopolysaccharidosis (MPS) is not well documented. We investigated aortic root measurements in 34 MPS patients at the Children's Hospital of Orange County (CHOC). The diagnosis, treatment status, age, gender, height, weight and aortic root parameters (aortic valve annulus (AVA), sinuses of Valsalva (SoV), and sinotubular junction (STJ)) were extracted by retrospective chart review and echocardiographic measurements. Descriptive statistics, ANOVA, and paired post-hoc t-tests were used to summarize the aortic dimensions. Exact binomial 95% confidence intervals (CIs) were constructed for ARD, defined as a z-score greater than 2 at the SoV. The patient age ranged from 3.4-25.9 years (mean 13.3 ± 6.1), the height from 0.87-1.62 meters (mean 1.24 ± 0.21), and the weight from 14.1-84.5 kg (mean 34.4 ± 18.0). The prevalence of dilation at the AVA was 41% (14/34; 95% CI: 25%-59%); at the SoV was 35% (12/34; 95% CI: 20%-54%); and at the STJ was 30% (9/30; 95% CI: 15%-49%). The highest prevalence of ARD was in MPS IVa (87.5%). There was no significant difference between mean z-scores of MPS patients who received treatment with hematopoietic stem cell transplantation (HSCT) or enzyme replacement therapy (ERT) vs. untreated MPS patients at the AVA (z = 1.9 ± 2.5 vs. z = 1.5 ± 2.4; p = 0.62), SoV (z = 1.2 ± 1.6 vs. z = 1.3 ± 2.2; p = 0.79), or STJ (z = 1.0 ± 1.8 vs. z = 1.2 ± 1.6; p = 0.83). The prevalence of ARD was 35% in our cohort of MPS I-VII patients. Thus, we recommend screening for ARD on a routine basis in this patient population.

  6. Aortic Root Dilatation in Mucopolysaccharidosis I–VII

    Science.gov (United States)

    Bolourchi, Meena; Renella, Pierangelo; Wang, Raymond Y.

    2016-01-01

    The prevalence of aortic root dilatation (ARD) in mucopolysaccharidosis (MPS) is not well documented. We investigated aortic root measurements in 34 MPS patients at the Children’s Hospital of Orange County (CHOC). The diagnosis, treatment status, age, gender, height, weight and aortic root parameters (aortic valve annulus (AVA), sinuses of Valsalva (SoV), and sinotubular junction (STJ)) were extracted by retrospective chart review and echocardiographic measurements. Descriptive statistics, ANOVA, and paired post-hoc t-tests were used to summarize the aortic dimensions. Exact binomial 95% confidence intervals (CIs) were constructed for ARD, defined as a z-score greater than 2 at the SoV. The patient age ranged from 3.4–25.9 years (mean 13.3 ± 6.1), the height from 0.87–1.62 meters (mean 1.24 ± 0.21), and the weight from 14.1–84.5 kg (mean 34.4 ± 18.0). The prevalence of dilation at the AVA was 41% (14/34; 95% CI: 25%–59%); at the SoV was 35% (12/34; 95% CI: 20%–54%); and at the STJ was 30% (9/30; 95% CI: 15%–49%). The highest prevalence of ARD was in MPS IVa (87.5%). There was no significant difference between mean z-scores of MPS patients who received treatment with hematopoietic stem cell transplantation (HSCT) or enzyme replacement therapy (ERT) vs. untreated MPS patients at the AVA (z = 1.9 ± 2.5 vs. z = 1.5 ± 2.4; p = 0.62), SoV (z = 1.2 ± 1.6 vs. z = 1.3 ± 2.2; p = 0.79), or STJ (z = 1.0 ± 1.8 vs. z = 1.2 ± 1.6; p = 0.83). The prevalence of ARD was 35% in our cohort of MPS I–VII patients. Thus, we recommend screening for ARD on a routine basis in this patient population. PMID:27916847

  7. DEVELOPMENT OF ENDF/B-VII.1 AND ITS COVARIANCE COMPONENT

    Energy Technology Data Exchange (ETDEWEB)

    Herman, M.

    2010-04-30

    The US nuclear data community, coordinated by CSEWG, is preparing release of the ENDF/B-VII.1 library. This new release will address deficiencies identified in ENDF/B-VII.0, include improved evaluations for some 50-60 materials and provide covariances for more than 110 materials. The major players in this undertaking are LANL, BNL, ORNL, and LLNL. We summarize deficiencies in the ENDF/B-VII.0 and outline development of the new library. We concentrate on the BNL activities which aim in providing covariances for the materials important for the design of the innovative reactors. Finally we outline a futuristic approach, known as assimilation that tries to link nuclear reaction theory and integral experiments.

  8. Validación de un ELISA para la cuantificación de las impurezas proteicas de la cepa hospedera en el principio activo de la vacuna recombinante cubana contra la hepatitis B

    Directory of Open Access Journals (Sweden)

    Eliana Pérez

    2014-04-01

    Full Text Available Se validó un inmunoensayo tipo sandwich de doble anticuerpo para cuantificar las impurezas proteicas de la cepa hospedera que pueden estar presentes en el principio activo de la vacuna cubana contra la hepatitis B. Se prepararon los reactivos biológicos empleados en el ELISA. Las proteínas de la cepa hospedera se obtuvieron bajo las mismas condiciones que el proceso de producción de la proteína recombinante hasta un paso de semipurificación o purificación primaria. Los antisueros dirigidos contra las proteínas de la cepa hospedera se generaron en conejos por un proceso de inmunización en cascada. Para la validación se analizaron los siguientes parámetros: linealidad, límite de detección y cuantificación, exactitud, rango y precisión. El ensayo establecido resultó específico, con una exactitud entre 89% y 109% para todos los tampones estudiados; se demostró un ajuste parabólico y un rango de trabajo entre 0,63 y 1,25 ng/mL, la repetibilidad y la precisión intermedia mostraron coeficientes de variación inferiores al 10 y 20% respectivamente.

  9. Establecimiento y validación de un ensayo inmunoenzimático tipo ELISA, empleado en el control de calidad del Interferón alfa 2b humano recombinante

    Directory of Open Access Journals (Sweden)

    Susset Valderrama

    2009-04-01

    Full Text Available Se estableció y validó un ELISA para la cuantificación del Interferón alfa 2b humano recombinante en el Ingrediente Farmacéutico Activo, definiéndose los criterios de validez del ensayo y basado en el cumplimiento de las Buenas Prácticas de Laboratorio. Se evaluaron los parámetros de: linealidad, especificidad, exactitud, rango, precisión y robustez. El ensayo establecido resultó específico, con una exactitud entre 84,3% y 102,4%, se demostró un ajuste cuadrático y un rango de trabajo entre 1 y 10 ng/mL; la repetibilidad y la precisión intermedia mostraron coeficientes de variación inferiores al 10% y 20% respectivamente, y se comprobó la robustez del método con respecto al efecto borde y la estabilidad de las soluciones.

  10. Recombinant human erythropoietin, and myocardial and cerebral acute ischemia: implications for clinical use. Eritropoyetina humana recombinante e isquemia aguda miocárdica y cerebral: sus implicaciones para el uso clínico

    Directory of Open Access Journals (Sweden)

    Maritza Cabrera Zamora

    2010-07-01

    Full Text Available Recombinant human erythropoietin has been used for more than two decades in clinical practice with promising results in the treatment of anemia associated with chronic renal insufficiency and in patients with cancer. Recent evidence has uncovered new nonhematopoietic functions of this protein and have brought new hope in the treatment of diseases with ischemic component. In the present review is rife with detail about these new features in the light of new discoveries and explores the therapeutic opportunities offered by these new scientific evidence.La eritropoyetina humana recombinante se ha empleado por más de dos décadas en la práctica clínica con resultados alentadores en el tratamiento de la anemia asociada a la insuficiencia renal crónica y en los pacientes con cáncer. Recientes evidencias han puesto al descubierto nuevas funciones no hematopoyéticas de esta proteína y han abierto nuevas esperanzas en la terapéutica de las enfermedades con componente isquémico. En la presente revisión se abunda con detalle sobre estas nuevas funciones, a la luz de los nuevos descubrimientos y se profundiza en las oportunidades terapéuticas que ofrecen estas nuevas evidencias científicas.

  11. Infecção experimental de bezerros com recombinantes do herpesvírus bovino tipo 5 defectivos na glicoproteína E (gE, timidina quinase (TK e ambos, gE/TK

    Directory of Open Access Journals (Sweden)

    C.M.B. Santos

    2011-04-01

    Full Text Available Este artigo descreve uma investigação da virulência/atenuação de recombinantes do herpesvírus bovino tipo 5 (BoHV-5 com deleções nos genes da glicoproteína E (BoHV-5gEΔ, timidina quinase (BoHV-5TKΔ, e ambos gE e TK (BoHV-5gEΔTKΔ. Bezerros soronegativos (80-90 dias de idade inoculados com o vírus parental SV-507/99 (n=5 excretaram o vírus em secreções nasais por até 15 dias (média de 10,8 dias. Nos animais inoculados com os recombinantes, a duração da excreção viral foi de 11 dias (BoHV-5gEΔ, 9,6 dias (BoHV-5TKΔ e 6,2 dias (BoHV-5gEΔTKΔ. Os maiores títulos foram observados entre os dias 1 e 6 pós-inoculação (pi, sendo de 10(6,8TCID50/mL para o SV-507/99, 10(5,1TCID50/mL (BoHV-5gEΔ, 10(5,9TCID50/mL (BoHV-5TKΔ e 10(4,7TCIΔ50/mL (BoHV-5gEΔTKΔ. Os bezerros inoculados com o vírus parental apresentaram anorexia e apatia; três deles mostraram apatia profunda e perda da condição corporal. Dois bezerros foram eutanasiados in extremis nos dias 10 e 11 pi, respectivamente e o vírus foi isolado de várias regiões do encéfalo. Já os bezerros inoculados com os recombinantes permaneceram saudáveis; alguns apresentaram uma secreção nasal serosa transitória. Administração de dexametasona (Dx no dia 42 pi resultou em excreção viral por todos os bezerros inoculados com o vírus parental (duração média de 3,7 dias, por 2 de 5 bezerros dos grupos BoHV-5TKΔ (dois dias e BoHV-5gEΔ (um dia. Os bezerros inoculados com o duplo mutante BoHV-5gEΔTKΔ não excretaram o vírus após o tratamento com Dx. Pesquisa de DNA viral por PCR no dia 30 pós-Dx revelou uma ampla distribuição do DNA do vírus parental no encéfalo; poucas seções (3/30 foram positivas no encéfalo dos animais do grupo BoHV-5gEΔ, e não detectou-se DNA latente no encéfalo dos animais dos grupos BoHV-5TKΔ e BoHV-5gEΔTKΔ. Esses resultados demonstram que os mutantes simples (gE and tk-deletados são atenuados para bezerros e estabelecem e

  12. Dietary changes in fasting levels of factor VII coagulant activity (FVII:C) are accompanied by changes in factor VII protein and other vitamin K-dependent proteins

    DEFF Research Database (Denmark)

    Bladbjerg, Else-Marie; Tholstrup, T; Marckmann, P

    1995-01-01

    each. Fasting blood samples were collected before and after the dietary regimen and analysed for triglycerides, FVII:C, and protein concentrations of FVII, FII, FX, protein C, CRP, albumin, fibrinogen, and F1 + 2. FVII:C was significantly reduced on diet S compared with diet ML. This was accompanied...... by a decrease in FVII protein, F1 + 2 and the vitamin K-dependent proteins FII, FX, and protein C. In contrast, no changes were observed in triglycerides, FVII:C/FVII:Ag, albumin and CRP. Fibrinogen was increased on diet S compared with diet ML. Our findings suggest that the change in fasting FVII:C was part...... of a general change in concentrations of vitamin K-dependent proteins. Udgivelsesdato: 1995-Feb...

  13. Chondroitin 6-Sulfate as a Novel Biomarker for Mucopolysaccharidosis IVA and VII.

    Science.gov (United States)

    Shimada, Tsutomu; Tomatsu, Shunji; Yasuda, Eriko; Mason, Robert W; Mackenzie, William G; Shibata, Yuniko; Kubaski, Francyne; Giugliani, Roberto; Yamaguchi, Seiji; Suzuki, Yasuyuki; Orii, Kenji; Orii, Tadao

    2014-01-01

    Chondroitin 6-sulfate (C6S), a glycosaminoglycan (GAG), is distributed mainly in the growth plates, aorta, and cornea; however, the physiological function of C6S is not fully understood. One of the limitations is that no rapid, accurate quantitative method to measure C6S has been established. Mucopolysaccharidosis IVA and VII (MPS IVA and VII) are caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase and β-D-glucuronidase, respectively, resulting in accumulation of C6S and other GAG(s). While levels of keratan sulfate (KS), heparan sulfate, and dermatan sulfate in samples from MPS patients are well described, this is the first report of quantitative analysis of C6S levels in samples from MPS IVA and VII patients.We developed a method to digest polymeric C6S and measure resultant disaccharides using liquid chromatography-tandem mass spectrometry (LC-MS/MS). C6S levels were measured in the blood from control subjects and patients with MPS IVA and VII aged from 0 to 58 years of age. We also assayed KS levels in the same samples for comparison with C6S.Levels of C6S in the blood decreased with age and were significantly elevated in patients with MPS IVA and VII, compared with age-matched controls. Levels of KS in patients with MPS IVA were also higher than those in age-matched controls, although differences were less pronounced than with C6S. Combining KS and C6S data, discriminated patients with MPS IVA from age-matched control subjects were better than either C6S or KS levels alone.In conclusion, this first report showing that blood levels of C6S are quantitatively evaluated in patients with MPS IVA and VII indicates that C6S could be a useful biomarker for these metabolic disorders.

  14. Dove sta andando l'analisi musicale? Riflessioni sul VII EuroMac

    Directory of Open Access Journals (Sweden)

    Mario Baroni

    2014-01-01

    Full Text Available Come tutti i lettori della Rivista ormai sapranno, si è svolto a Roma dal 29 settembre al 2 ottobre di quest’anno, presso il Conservatorio di S. Cecilia, il settimo convegno europeo di analisi musicale (VII European Music Analysis Conference ossia VII EuroMac. Nel convegno, organizzato dal GATM e diretto da Giorgio Sanguinetti, sono state presentate 197 relazioni e svolti otto laboratori. Tutti i materiali sono stati riassunti in un volume (Programme and Abstract Book, Iter Edizioni di Subiaco curato da Egidio Pozzi e Antonio Cascelli.

  15. ESEA Title VII Bilingual/Bicultural Education Program; Programa de Educacion Bilingue/Bicultural 1975-1976.

    Science.gov (United States)

    Milwaukee Public Schools, WI. Dept. of Educational Research and Program Assessment.

    This is the annual evaluation report of the Title VII components of the Milwaukee Bilingual/Bicultural Education Program funded by Title I and Title VII of the Elementary and Secondary Education Act of 1967 and the Milwaukee Public Schools. The results of the 1975-76 assessment of pupil progress toward achievement of the program's academic and…

  16. Conserved water-mediated hydrogen bond network between TM-I, -II, -VI, and -VII in 7TM receptor activation

    DEFF Research Database (Denmark)

    Nygaard, Rie; Hansen, Louise Valentin; Mokrosinski, Jacek;

    2010-01-01

    Five highly conserved polar residues connected by a number of structural water molecules together with two rotamer micro-switches, TrpVI:13 and TyrVII:20, constitute an extended hydrogen bond network between the intracellular segments of TM-I, -II, -VI, and -VII of 7TM receptors. Molecular dynamics...... to apparently function as a catching trap for water molecules. Mutational analysis of the beta2-adrenergic receptor demonstrated that the highly conserved polar residues of the hydrogen bond network were all important for receptor signaling but served different functions, some dampening constitutive activity...... (AsnI:18, AspII:10, and AsnVII:13), whereas others (AsnVII:12 and AsnVII:16) located one helical turn apart and sharing a water molecule were shown to be essential for agonist-induced signaling. It is concluded that the conserved water hydrogen bond network of 7TM receptors constitutes an extended...

  17. Purification and characterization of adenovirus core protein VII: a histone-like protein that is critical for adenovirus core formation.

    Science.gov (United States)

    Sharma, Gaurav; Moria, Nithesh; Williams, Martin; Krishnarjuna, Bankala; Pouton, Colin W

    2017-07-01

    Adenovirus protein VII is a highly cationic core protein that forms a nucleosome-like structure in the adenovirus core by condensing DNA in combination with protein V and mu. It has been proposed that protein VII could condense DNA in a manner analogous to mammalian histones. Due to the lack of an expression and purification protocol, the interactions between protein VII and DNA are poorly understood. In this study we describe methods for the purification of biologically active recombinant protein VII using an E. coli expression system. We expressed a cleavable fusion of protein VII with thioredoxin and established methods for purification of this fusion protein in denatured form. We describe an efficient method for resolving the cleavage products to obtain pure protein VII using hydroxyapatite column chromatography. Mass spectroscopy data confirmed its mass and purity to be 19.4 kDa and >98 %, respectively. Purified recombinant protein VII spontaneously condensed dsDNA to form particles, as shown by dye exclusion assay, electrophoretic mobility shift assay and nuclease protection assay. Additionally, an in vitro bioluminescence assay revealed that protein VII can be used to enhance the transfection of mammalian cells with lipofectamine/DNA complexes. The availability of recombinant protein VII will facilitate future studies of the structure of the adenovirus core. Improved understanding of the structure and function of protein VII will be valuable in elucidating the mechanism of adenoviral DNA condensation, defining the morphology of the adenovirus core and establishing the mechanism by which adenoviral DNA enters the nucleus.

  18. Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum Isolamento de linhagens recombinantes com maior produção de pectinases por meio de fusão de protoplastos entre Penicillium expansum e Penicillium griseoroseum

    Directory of Open Access Journals (Sweden)

    Maurilio Antonio Varavallo

    2007-03-01

    Full Text Available Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+. Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum parental strain, presented a considerable increase in polygalacturonase (3-fold and pectin lyase production (1.2-fold.Fusões de protoplastos entre linhagens mutantes auxotróficas e morfológicas complementares de Penicillium griseoroseum e P. expansum foram induzidas por polietilenoglicol e íons cálcio (Ca2+. Fusionantes foram obtidos em meio mínimo e uma linhagem prototrófica, possivelmente diplóide, foi selecionada para a haploidização com o fungicida benomil. Diferentes linhagens recombinantes foram isoladas e caracterizadas quanto à presença de mutações auxotróficas e a produção de enzimas pectinolíticas. O fusionante prototrófico não apresentou maior atividade de pectinases em relação às linhagens parentais, entretanto, entre 29 recombinantes analisados, quatro apresentaram maiores atividades enzimáticas. O recombinante RGE27, o qual possui as mesmas mutações auxotróficas e morfológicas que a linhagem parental de P. griseoroseum, apresentou um aumento considerável na produção de poligalacturonase (3 vezes e de pectina liase (1,2 vezes.

  19. FUNDAMENTALS OF AMHARIC (REVISED), UNITS IV-VII (LESSONS 16-35).

    Science.gov (United States)

    BARTON, DONALD K.; AND OTHERS

    THIS IS THE SECOND OF A THREE-VOLUME COURSE IN BASIC AMHARIC. FOLLOWING THE SAME FORMAT AS THE FIRST VOLUME, EACH LESSON IN UNITS IV-VII CONTAINS A DIALOG, GRAMMATICAL NOTES, PATTERN DRILLS, EXERCISES, AND A VOCABULARY LIST. ENGLISH-AMHARIC AND AMHARIC-ENGLISH GLOSSARIES ARE APPENDED. THIS COURSE WILL PREPARE THE STUDENT TO PERFORM AT A…

  20. Anticancer Effects of Sinocrassulosides VI/VII from Silene viscidula on HeLa Cells

    Directory of Open Access Journals (Sweden)

    Hang Chen

    2017-01-01

    Full Text Available Natural products are becoming increasingly important in chemoprevention and for cancer therapy. Silene viscidula (S. viscidula, a traditional Chinese herb, has long been used as an anti-inflammatory and neuroleptic agent. However, the anticancer activity of S. viscidula has remained unclear. In this study, 16 compounds were extracted from S. viscidula. Among those compounds, sinocrassulosides VI/VII, an inseparable isomer mixture, possess the strongest inhibitory activity on HeLa cells with the IC50 value of 2.37 μM. Mechanism studies found that sinocrassulosides VI/VII downregulated the expression of cyclin D1 and decreased retinoblastoma (Rb phosphorylation, which arrested HeLa cells in the G1 phase. Also, sinocrassulosides VI/VII could induce senescence via the upregulation of p16 and a significant increase of β-galactosidase (β-gal staining. Our results suggest that sinocrassulosides VI/VII may be a new therapeutic potential agent for cervical cancer. In addition, we explored the structure-activity relationships of three groups of the configurational isomer with similar chemical structure from S. viscidula. We first demonstrated that the length of the ester chains linked to the carboxyl group of the glucuronic acid residue could affect the potent cytotoxicity. This finding will open new avenues for developing effective anticancer compounds by modifying the components derived from plants in nature.

  1. Anticancer Effects of Sinocrassulosides VI/VII from Silene viscidula on HeLa Cells.

    Science.gov (United States)

    Chen, Hang; Ma, Qian; Xu, Wei; Li, Wan-Ming; Yuan, De-Zheng; Wu, Jia-Mei; Li, Yu-Shan; Fang, Jin

    2017-01-01

    Natural products are becoming increasingly important in chemoprevention and for cancer therapy. Silene viscidula (S. viscidula), a traditional Chinese herb, has long been used as an anti-inflammatory and neuroleptic agent. However, the anticancer activity of S. viscidula has remained unclear. In this study, 16 compounds were extracted from S. viscidula. Among those compounds, sinocrassulosides VI/VII, an inseparable isomer mixture, possess the strongest inhibitory activity on HeLa cells with the IC50 value of 2.37 μM. Mechanism studies found that sinocrassulosides VI/VII downregulated the expression of cyclin D1 and decreased retinoblastoma (Rb) phosphorylation, which arrested HeLa cells in the G1 phase. Also, sinocrassulosides VI/VII could induce senescence via the upregulation of p16 and a significant increase of β-galactosidase (β-gal) staining. Our results suggest that sinocrassulosides VI/VII may be a new therapeutic potential agent for cervical cancer. In addition, we explored the structure-activity relationships of three groups of the configurational isomer with similar chemical structure from S. viscidula. We first demonstrated that the length of the ester chains linked to the carboxyl group of the glucuronic acid residue could affect the potent cytotoxicity. This finding will open new avenues for developing effective anticancer compounds by modifying the components derived from plants in nature.

  2. Vittorio De Sica viis meistritööd / Aare Ermel

    Index Scriptorium Estoniae

    Ermel, Aare, 1957-2013

    2008-01-01

    DVD-st "Vittorio De Sica Masterpiece Collection", kus on peal itaalia filmirežissööri Vittorio De Sica viis teost: "Jalgratavargad" ("Ladri di biciclette", 1948), "Ime Milanos" ("Miraclo a milano", 1951), "Umberto D." (1952), "Eile täna, homme" ("Ieri, oggi, domani", 1963), "Ajatu filmikunst" ("Cinema senza tempo: Cosi e la vita", 2001)

  3. Dos Idiomas, Un Mundo. Dual Language Project. Title VII Biennial Evaluation Report, 1995-97.

    Science.gov (United States)

    Ernest, Harishini M.; Gonzalez, Rosa M.

    This is an evaluation of the first 2 years of a 5-year comprehensive Bilingual Education grant funded by Title VII Part A of the Improving America's Schools Act of 1994 in the Austin Independent School District (AISD) (Texas). The grant awarded to the AISD funds a program of Developmental Bilingual Education at two elementary schools where more…

  4. Quantitative clinical characteristics of 53 patients with MPS VII: a cross-sectional analysis.

    Science.gov (United States)

    Zielonka, Matthias; Garbade, Sven F; Kölker, Stefan; Hoffmann, Georg F; Ries, Markus

    2017-04-06

    The main purpose of the study was to provide quantitative data regarding survival and diagnostic delay. Mucopolysaccharidosis (MPS) type VII (OMIM 253220) is a progressive neurometabolic disorder caused by deficiency of the lysosomal enzyme β-glucuronidase (GUS). Hard clinical end points have not been quantitatedMethods:We quantitatively analyzed published cases with MPS VII (N = 53/88 with sufficient data). Main outcome measures were onset of disease and survival. The role of biomarkers such as GUS residual enzyme activity and levels of storage material assessed as urinary excretion of glucosaminoglycans (GAG) as potential predictors of clinical outcomes were investigated. The analysis was conducted according to STROBE criteria. Median survival of the postnatally diagnosed population was 42 months. Median age of disease onset was the first day of life; median age at diagnosis was 11 months. Hydrops fetalis was frequent. Patients with residual GUS activity in fibroblasts more than 1.4% or urinary GAG excretion less than 602% of normal survived longer than patients with GUS enzyme activity below or GAG excretion above these thresholds. MPS VII has its disease onset prenatally. In the absence of a prenatal diagnosis, most cases are clinically apparent at birth. Our data corroborate a phenotype-biomarker association in MPS VII. The survival data characterize the natural history with important implications for therapeutic studies.Genet Med advance online publication 06 April 2017Genetics in Medicine (2017); doi:10.1038/gim.2017.10.

  5. Presidendi teenetemärgi pälvis viis spordiinimest

    Index Scriptorium Estoniae

    2008-01-01

    Vt. ka Linnaleht : Tartu 7. veebr., lk. 7. Eesti riigile osutatud teenete eest pälvisid president Toomas Hendrik Ilveselt teenetemärgi viis spordieluga seotud inimest: jalgpallikoondise ekstreenerid Jelle Goes ja Arno Pijpers, kergejõustikutreener Vésteinn Hafsteinsson, sõudetreener Tatjana Jaanson ning spordielu edendaja Nora Kutti. Juuresoleval fotol Jelle Goes

  6. 78 FR 36011 - Region VII Regulatory Fairness Board; Federal Regulatory Enforcement Fairness Hearing

    Science.gov (United States)

    2013-06-14

    ... ADMINISTRATION Region VII Regulatory Fairness Board; Federal Regulatory Enforcement Fairness Hearing AGENCY: U.S... Business Regulatory Fairness Board. SUMMARY: The (SBA) Office of the National Ombudsman is issuing this notice to announce the location, date and time of the Regional Small Business Regulatory Fairness hearing...

  7. MERLIN and VLA Observations of VII Zw 19: Distant Cousin of M82

    Science.gov (United States)

    Beck, Sara C.; Garrington, S. T.; Turner, Jean L.; Van Dyk, Schuyler D.

    2004-10-01

    We have observed the starburst dwarf galaxy VII Zw 19 at subarcsecond resolution with MERLIN and MERLIN+European VLBI Network (EVN) at 18 cm, with MERLIN at 6 cm, and with the Very Large Array (VLA) in the A configuration at 6 and 2 cm. The galaxy is detected at all VLA wavelengths and is resolved. It is also resolved at 18 cm by MERLIN but is not detected by the EVN or by MERLIN at 6 cm. VII Zw 19 has a complex structure of nonthermal radio emission at 18 cm extended over ~1200-1800 pc (4"-6"). That the EVN did not detect this emission indicates that there are no obvious point sources that could be radio supernovae. The 2 cm emission, predominantly thermal free-free emission, has a markedly different spatial distribution from the nonthermal emission. The radio colors show that the galaxy contains numerous supernova remnants, as well as many young H II regions, many of which are optically thick at 6 cm. Two extended regions of 2 cm emission have little 18 cm flux and are probably emission from young star clusters. VII Zw 19 resembles M82 in its radio and infrared spectrum, but the starburst region in VII Zw 19 is twice the size of the one in M82 and twice as luminous.

  8. Vittorio De Sica viis meistritööd / Aare Ermel

    Index Scriptorium Estoniae

    Ermel, Aare, 1957-2013

    2008-01-01

    DVD-st "Vittorio De Sica Masterpiece Collection", kus on peal itaalia filmirežissööri Vittorio De Sica viis teost: "Jalgratavargad" ("Ladri di biciclette", 1948), "Ime Milanos" ("Miraclo a milano", 1951), "Umberto D." (1952), "Eile täna, homme" ("Ieri, oggi, domani", 1963), "Ajatu filmikunst" ("Cinema senza tempo: Cosi e la vita", 2001)

  9. Correction of murine mucopolysaccharidosis VII by a human. beta. -glucuronidase transgene

    Energy Technology Data Exchange (ETDEWEB)

    Kyle, J.W.; Vogler, C.; Hoffmann, J.W.; Sly, W.S. (St. Louis Univ. School of Medicine, MO (USA)); Birkenmeier, E.H.; Gwynn, B. (Jackson Laboratory, Bar Harbor, ME (USA))

    1990-05-01

    The authors recently described a murine model for mucopolysaccharidosis VII in mice that have an inherited deficiency of {beta}-glucuronidase. Affected mice, of genotype gus{sup mps}/gus{sup mps}, present clinical manifestations similar to those of humans with mucopolysaccharidosis VII (Sly syndrome) and are shown here to have secondary elevations of other lysosomal enzymes. The mucopolysaccharidosis VII phenotype in both species includes dwarfism, skeletal deformities, and premature death. Lysosome storage is visualized within enlarged vesicles and correlates biochemically with accumulation of undegraded and partially degraded glycosaminoglycans. In this report they describe the consequences of introducing the human {beta}-glucuronidase gene, GUSB, into gus{sup mps}/gus{sup mps} mice that produce virtually no murine {beta}-glucuronidase. Transgenic mice homozygous for the mucopolysaccharidosis VII mutation expressed high levels of human {beta}-glucuronidase activity in all tissues examined and were phenotypically normal. Biochemically, both the intralysosomal storage of glycosaminoglycans and the secondary elevation of other acid hydrolases were corrected. These findings demonstrate that the GUSB transgene is expressed in gus{sup mps}/gus{sup mps} mice and that human {beta}-glucuronidase corrects the murine mucopolysaccharidosis storage disease.

  10. VVER-440 and VVER-1000 reactor dosimetry benchmark - BUGLE-96 versus ALPAN VII.0

    Energy Technology Data Exchange (ETDEWEB)

    Duo, J. I. [Radiation Engineering and Analysis, Westinghouse Electric Company LLC, 1000 Westinghouse Dr., Cranberry Township, PA 16066 (United States)

    2011-07-01

    Document available in abstract form only, full text of document follows: Analytical results of the vodo-vodyanoi energetichesky reactor-(VVER-) 440 and VVER-1000 reactor dosimetry benchmarks developed from engineering mockups at the Nuclear Research Inst. Rez LR-0 reactor are discussed. These benchmarks provide accurate determination of radiation field parameters in the vicinity and over the thickness of the reactor pressure vessel. Measurements are compared to calculated results with two sets of tools: TORT discrete ordinates code and BUGLE-96 cross-section library versus the newly Westinghouse-developed RAPTOR-M3G and ALPAN VII.0. The parallel code RAPTOR-M3G enables detailed neutron distributions in energy and space in reduced computational time. ALPAN VII.0 cross-section library is based on ENDF/B-VII.0 and is designed for reactor dosimetry applications. It uses a unique broad group structure to enhance resolution in thermal-neutron-energy range compared to other analogous libraries. The comparison of fast neutron (E > 0.5 MeV) results shows good agreement (within 10%) between BUGLE-96 and ALPAN VII.O libraries. Furthermore, the results compare well with analogous results of participants of the REDOS program (2005). Finally, the analytical results for fast neutrons agree within 15% with the measurements, for most locations in all three mockups. In general, however, the analytical results underestimate the attenuation through the reactor pressure vessel thickness compared to the measurements. (authors)

  11. Structural studies on metal-containing enzymes. T4 endonuclease VII and D. gigas formate dehydrogenase

    NARCIS (Netherlands)

    Raaijmakers, H.C.A.

    2001-01-01

    Many biological processes require metal ions, and many of these metal-ion functions involve metalloproteins. The metal ions in metalloproteins are often critical to the protein's function, structure, or stability. This thesis focuses on two of these proteins, bacteriophage T4 endonuclease VII (EndoV

  12. Presidendi teenetemärgi pälvis viis spordiinimest

    Index Scriptorium Estoniae

    2008-01-01

    Vt. ka Linnaleht : Tartu 7. veebr., lk. 7. Eesti riigile osutatud teenete eest pälvisid president Toomas Hendrik Ilveselt teenetemärgi viis spordieluga seotud inimest: jalgpallikoondise ekstreenerid Jelle Goes ja Arno Pijpers, kergejõustikutreener Vésteinn Hafsteinsson, sõudetreener Tatjana Jaanson ning spordielu edendaja Nora Kutti. Juuresoleval fotol Jelle Goes

  13. Prevalence of collagen VII-specific autoantibodies in patients with autoimmune and inflammatory diseases

    Directory of Open Access Journals (Sweden)

    Licarete Emilia

    2012-04-01

    Full Text Available Abstract Background Autoimmunity to collagen VII is typically associated with the skin blistering disease epidermolysis bullosa acquisita (EBA, but also occurs occasionally in patients with systemic lupus erythematosus or inflammatory bowel disease. The aim of our present study was to develop an accurate immunoassay for assessing the presence of autoantibodies against collagen VII in large cohorts of patients and healthy donors. Methods Based on in silico antigenic analysis and previous wetlab epitope mapping data, we designed a chimeric collagen VII construct containing all collagen VII epitopes with higher antigenicity. ELISA was performed with sera from patients with EBA (n = 50, Crohn's disease (CD, n = 50, ulcerative colitis (UC, n = 50, bullous pemphigoid (BP, n = 76, and pemphigus vulgaris (PV, n = 42 and healthy donors (n = 245. Results By ELISA, the receiver operating characteristics analysis yielded an area under the curve of 0.98 (95% CI: 0.9638-1.005, allowing to set the cut-off at 0.32 OD at a calculated specificity of 98% and a sensitivity of 94%. Running the optimized test showed that serum IgG autoantibodies from 47 EBA (94%; 95% CI: 87.41%-100%, 2 CD (4%; 95% CI: 0%-9.43%, 8 UC (16%; 95% CI: 5.8%-26%, 2 BP (2.63%; 95% CI: 0%-6.23%, and 4 PV (9.52%; 95% CI: 0%-18.4% patients as well as from 4 (1.63%; 95% CI: 0%-3.21% healthy donors reacted with the chimeric protein. Further analysis revealed that in 34%, 37%, 16% and 100% of sera autoantibodies of IgG1, IgG2, IgG3, and IgG4 isotype, respectively, recognized the recombinant autoantigen. Conclusions Using a chimeric protein, we developed a new sensitive and specific ELISA to detect collagen specific antibodies. Our results show a low prevalence of collagen VII-specific autoantibodies in inflammatory bowel disease, pemphigus and bullous pemphigoid. Furthermore, we show that the autoimmune response against collagen VII is dominated by IgG4 autoantibodies. The new immunoassay should

  14. Neutron Thermal Cross Sections, Westcott Factors, Resonance Integrals, Maxwellian Averaged Cross Sections and Astrophysical Reaction Rates Calculated from the ENDF/B-VII.1, JEFF-3.1.2, JENDL-4.0, ROSFOND-2010, CENDL-3.1 and EAF-2010 Evaluated Data Libraries

    Science.gov (United States)

    Pritychenko, B.; Mughabghab, S. F.

    2012-12-01

    We present calculations of neutron thermal cross sections, Westcott factors, resonance integrals, Maxwellian-averaged cross sections and astrophysical reaction rates for 843 ENDF materials using data from the major evaluated nuclear libraries and European activation file. Extensive analysis of newly-evaluated neutron reaction cross sections, neutron covariances, and improvements in data processing techniques motivated us to calculate nuclear industry and neutron physics quantities, produce s-process Maxwellian-averaged cross sections and astrophysical reaction rates, systematically calculate uncertainties, and provide additional insights on currently available neutron-induced reaction data. Nuclear reaction calculations are discussed and new results are presented. Due to space limitations, the present paper contains only calculated Maxwellian-averaged cross sections and their uncertainties. The complete data sets for all results are published in the Brookhaven National Laboratory report.

  15. Neutron Thermal Cross Sections, Westcott Factors, Resonance Integrals, Maxwellian Averaged Cross Sections and Astrophysical Reaction Rates Calculated from the ENDF/B-VII.1, JEFF-3.1.2, JENDL-4.0, ROSFOND-2010, CENDL-3.1 and EAF-2010 Evaluated Data Libraries

    Energy Technology Data Exchange (ETDEWEB)

    Pritychenko, B. [National Nuclear Data Center, Brookhaven National Laboratory, Upton, NY 11973-5000 (United States); Mughabghab, S.F. [National Nuclear Data Center, Brookhaven National Laboratory, Upton, NY 11973-5000 (United States)

    2012-12-15

    We present calculations of neutron thermal cross sections, Westcott factors, resonance integrals, Maxwellian-averaged cross sections and astrophysical reaction rates for 843 ENDF materials using data from the major evaluated nuclear libraries and European activation file. Extensive analysis of newly-evaluated neutron reaction cross sections, neutron covariances, and improvements in data processing techniques motivated us to calculate nuclear industry and neutron physics quantities, produce s-process Maxwellian-averaged cross sections and astrophysical reaction rates, systematically calculate uncertainties, and provide additional insights on currently available neutron-induced reaction data. Nuclear reaction calculations are discussed and new results are presented. Due to space limitations, the present paper contains only calculated Maxwellian-averaged cross sections and their uncertainties. The complete data sets for all results are published in the Brookhaven National Laboratory report.

  16. Results of thrombolytic treatment with recombinant streptokinase in elderly and non elderly patients. Resultados del tratamiento trombolítico con estreptoquinasa recombinante en pacientes ancianos y no ancianos.

    Directory of Open Access Journals (Sweden)

    Marcos D. Iraola Ferrer

    resultados del tratamiento con estreptoquinasa recombinante, en ancianos y no ancianos con infarto agudo del miocadio. Métodos: Estudio prospectivo observacional de 325 pacientes ingresados con infarto agudo del miocardio en el Hospital Provincial Universitario ¨Dr. Gustavo Aldereguìa Lima¨, de Cienfuegos, que recibieron terapéutica trombolítica con estreptoquinasa recombinante ( Heberquinasa a la dosis de 1 500 000 unidades, y tratamiento conjunto con 325 mg de ácido acetilsalicìlico. Resultados: La presencia de infarto fue más frecuente en el sexo masculino (68,31 %; asociado en mayor porcentaje al tabaquismo, la hipertensión arterial, la hipercolesteronemia en los pacientes menores de 74 años ; la hipertensión arterial y la diabetes en los mayores de 75 años; la cara anterior (44,6 %, fue la más afectada y la terapia trombolítica se realizó con una media de 211, 76 minutos. Los fallecidos aumentaron con la edad. El hecho de estar clasificado hemodinámicamente en un Killip-Kimball (K-K grado III –IV, haber tenido arritmias graves y rotura cardiaca fueron la variables que se asociaron de forma significativa con la mortalidad.

  17. Genetic diversity of the genotype VII Newcastle disease virus: identification of a novel VIIj sub-genotype.

    Science.gov (United States)

    Xue, Cong; Cong, Yanlong; Yin, Renfu; Sun, Yixue; Ding, Chan; Yu, Shengqing; Liu, Xiufan; Hu, Shunlin; Qian, Jing; Yuan, Qianliang; Yang, Mingxi; Wang, Chunfeng; Ding, Zhuang

    2017-02-01

    Newcastle disease (ND) is a highly contagious disease of poultry caused by Newcastle disease virus (NDV). Multiple genotypes of NDV have been circulating worldwide and NDV is continuously evolving, resulting into more diversity. Of multiple viral genotypes, VII is particularly important given that it had been associated with most recent ND outbreaks worldwide. In this study, an epidemiological investigation performed in northeastern China during 2014-2015 showed that 11 genotype VII isolates amounted to 55 percent in a total number of NDV isolates. Therefore, to evaluate the genetic diversity worldwide and epidemiological distribution in China of genotype VII NDV, a phylogenetic analysis based on the 1255 complete F gene sequences showed that VII is the most predominant genotype worldwide. A further detailed characterization on genotype VII was conducted based on the 477 complete F gene sequences from 11 isolates and 466 reference viruses available in GenBank. The results demonstrated that VII can be further divided into 8 sub-genotypes (VIIb, VIId-VIIj), indicating its complex genetic diversity. It is worthy of note that the isolation rate of VIIj is increasing recently. It emphasizes the necessity to pay close attention to the epidemiological dynamic of genotype VII NDV and highlights the importance of vaccination program.

  18. Role of lysine and tryptophan residues in the biological activity of toxin VII (Ts gamma) from the scorpion Tityus serrulatus.

    Science.gov (United States)

    Hassani, O; Mansuelle, P; Cestèle, S; Bourdeaux, M; Rochat, H; Sampieri, F

    1999-02-01

    Toxin VII (TsVII), also known as Ts gamma, is the most potent neurotoxin in the venom of the Brazilian scorpion Tityus serrulatus. It has been purified to homogeneity using a new fast and efficient method. Chemical modification of TsVII with the tryptophan-specific reagent o-nitrophenylsulfenyl chloride yielded three modified derivatives (residues Trp39, Trp50 and Trp54). Acetylation of TsVII mostly generated the monoacetylated Lys12 derivative. No side reactions were detected, as indicated by endoproteinase Lys-C peptide mapping, Edman degradation and electrospray mass spectrometry. Circular dichroism and fluorimetric measurements showed that none of the chemical modifications altered the overall structure of the derivatives. The acetylation of Lys12 or the sulfenylation of Trp39 or Trp54 led to a loss of both toxicity in mice and apparent binding affinity for rat brain and cockroach synaptosomal preparations. Sulfenylation of Trp50, however, moderately affected the toxicity of TsVII in mice and had almost no effect on its binding properties. A 3-dimensional model of TsVII was constructed by homology modeling. It suggests that the most reactive residues (Lys12 and Trp39 and Trp54) are all important in the functional disruption of neuronal sodium channels by TsVII, and are close to each other in the hydrophobic conserved region.

  19. Riesgo de exposición a Fasciola hepática en ganado vacuno en extensivo de Uruguay y Portugal determinado mediante ELISA y un antígeno recombinante

    Directory of Open Access Journals (Sweden)

    J Sanchís

    2015-01-01

    Full Text Available Para evaluar el riesgo de exposición a Fasciola hepatica del ganado vacuno en extensivo se tomaron muestras de sangre de dos zonas climáticas diferentes, Uruguay (clima subtropical húmedo, 1.192 muestras y Portugal (clima mediterráneo, 473 muestras, que se analizaron con un ELISA y un antígeno recombinante de superficie de F. hepatica (FhrAPS. Los datos obtenidos se analizaron de acuerdo con la raza, edad sexo y aptitud. La seroprevalencia global en Uruguay resultó del 56% (Intervalo de Confianza, IC 95%: 53-59 mediante FhrAPS-ELISA, con porcentajes significativamente más altos en vacas lecheras Holstein de 2-4 años. En Portugal, el porcentaje de sensibilización fue del 47% (IC 95%: 43-52 con los valores más elevados en bovinos resultado de cruces de distintas razas, destinados a la obtención de carne y con edad también comprendida entre 2 y 4 años. Se concluyó que el riesgo de exposición a F. hepatica del ganado vacuno en extensivo es mayor en las zonas con clima subtropical húmedo que en las de clima mediterráneo. Los terneros presentan un alto riesgo de exposición al trematodo hepático con independencia de las condiciones climáticas. Los resultados ponen de manifiesto la necesidad de adoptar medidas frente a las formas del parásito que se encuentran en el medio y combinarlas con programas de desparasitación eficaces.

  20. Caracterização de linhagens endogâmicas recombinantes e mapeamento de locos de características quantitativas associados a ciclo e produtividade do feijoeiro-comum

    Directory of Open Access Journals (Sweden)

    Faleiro Fábio Gelape

    2003-01-01

    Full Text Available O objetivo deste trabalho foi caracterizar 154 linhagens endogâmicas recombinantes por meio da avaliação de características quantitativas, morfológicas, moleculares e de resistência a doenças e mapear locos de características quantitativas associados a ciclo e produtividade do feijoeiro-comum. Adotando o valor do limite de detecção (LOD de 4,0 e uma freqüência máxima de recombinação de 0,40, foram mapeados 43 marcadores em nove grupos de ligação cobrindo uma distância de recombinação total de 247,8 cM. A distância entre marcadores adjacentes variou entre 0 e 28 cM, com média de 7,3 cM. Os grupos de ligação variaram em tamanho de 2,3 a 61,2 cM. Os genes de resistência à ferrugem e à antracnose ficaram localizados no mesmo grupo de ligação. Foram mapeados locos associados às oito características quantitativas estudadas, e a explicação da variância fenotípica pelos marcadores variou de 14,03% a 40,14%. Os resultados encontrados lançam bases para o desenvolvimento de mapas específicos saturados e de utilidade em programas de melhoramento do feijoeiro-comum.

  1. Procedimiento para la producción de sacarosa sintasa recombinante, su uso en la fabricación de kits de determinación de sacarosa, método de producción de ADPglucosa y método de obtención de plantas transgénicas cuyas hojas y órganos de reserva acumulen alto contenido en ADPglucosa y almidón

    OpenAIRE

    Baroja-Fernández, Edurne; Muñoz Pérez, Francisco José; Pozueta Romero, Javier; Morán Zorzano, María Teresa; Alonso Casajús, Nora

    2004-01-01

    Procedimiento para la producción de sacarosa sintasa recombinante, su uso en la fabricación de kits de determinación de sacarosa, método de producción de ADPglucosa y método de obtención de plantas transgénicas cuyas hojas y órganos de reserva acumulen alto contenido en ADPglucosa y almidón.

  2. Phase Relations and Properties of Salty ices VI and VII: Implications for Solar System Ices

    Science.gov (United States)

    Daniel, I.; Manning, C. E.

    2008-12-01

    Ice VI and ice VII may be important in the interiors of Europa, Ganymede, Callisto and Titan. Oceans and interior pore waters in these bodies likely contain dissolved salts. To address the role of salt on ice VI and ice VII, we investigated phase equilibria in the system H2O -NaCl at 1 molal (5.5 wt%) NaCl in an externally heated diamond-anvil cell. Phase identifications were made by optical microscopy combined with Raman spectroscopy. Experiments were conducted at 22-150°C and up to 5 GPa by allowing the cell to thermally equilibrate at a given temperature and then varying pressure isothermally while observing phase changes. The liquidus curves of ice VI and ice VII in a 5.5 wt% NaCl solution were determined. Melting was observed from 22 to 80°C (ice VI) and from 35 to 150°C (ice VII). Both melting curves are steeper than the respective NaCl-free curves, indicating that the freezing-point depression at this bulk composition increases with pressure. The intersection of the two liquidus curves indicates that VI-VII-liquid triple point is shifted toward lower T and higher P relative to pure H2O. The 5.5 wt% NaCl bulk composition crystallizes into a single solid phase of NaCl-bearing ice VI or ice VII solid solution over the investigated T range (the subscript 'ss' indicates solid solution). Large single crystals of ice VIss or ice VIIss can also be grown by slow compression of the cell from near-liquidus conditions to the solidus. Raman spectra of these crystals clearly show zoning in these crystals. The zoning persists for days at 22°C, indicating relatively slow Na+ and Cl- diffusivity. The large depression of the freezing point in a 1 molal NaCl solution has important implications for the oceans and interiors of the icy satellites of Jupiter and Saturn. Salty fluids may remain stable to much greater depth than expected. This would promote extensive hydrothermal metamorphism of the silicate interiors. If not limited to ice VI and VII, this behavior may suppress

  3. Type VII Secretion Systems in Gram-Positive Bacteria

    NARCIS (Netherlands)

    Bottai, Daria; Gröschel, Matthias I.; Brosch, Roland; Bagnoli, Fabio; Rappuoli, Rino

    2017-01-01

    Bacterial secretion systems are sophisticated molecular machines that fulfil a wide range of important functions, which reach from export/secretion of essential proteins or virulence factors to the implication in conjugation processes. In contrast to the widely distributed Sec and Twin Arginine

  4. A thymidine kinase-deleted bovine herpesvirus 5 establishes latent infection but reactivates poorly in a sheep model Recombinante do herpesvírus bovino tipo 5 com deleção na timidina quinase estabelece infecção latente porém reativa ineficientemente em ovinos

    Directory of Open Access Journals (Sweden)

    Gustavo C. Cadore

    2013-03-01

    Full Text Available The ability of thymidine kinase (tk-deleted recombinant bovine herpesvirus 5 (BoHV-5tkΔ to establish and reactivate latent infection was investigated in lambs. During acute infection, the recombinant virus replicated moderately in the nasal mucosa, yet to lower titers than the parental strain. At day 40 post-infection (pi, latent viral DNA was detected in trigeminal ganglia (TG of all lambs in both groups. However, the amount of recombinant viral DNA in TGs was lower (9.7-fold less than that of the parental virus as determined by quantitative real time PCR. Thus, tk deletion had no apparent effect on the frequency of latent infection but reduced colonization of TG. Upon dexamethasone (Dx administration at day 40 pi, lambs inoculated with parental virus shed infectious virus in nasal secretions, contrasting with lack of infectivity in secretions of lambs inoculated with the recombinant virus. Nevertheless, some nasal swabs from the recombinant virus group were positive for viral DNA by PCR, indicating low levels of reactivation. Thus, BoHV-5 TK activity is not required for establishment of latency, but seems critical for efficient virus reactivation upon Dx treatment.A capacidade de um recombinante do herpesvírus bovino tipo 5 com deleção no gene da timidina quinase (BoHV-5tk∆ em estabelecer e reativar infecção latente foi investigada em cordeiros. Durante a infecção aguda, o vírus recombinante replicou na mucosa nasal em títulos moderados, porém menores do que os da cepa parental. Aos 40 dias pós-infecção (pi DNA viral latente foi detectado no gânglio trigêmeo (TG de todos os cordeiros em ambos os grupos. No entanto, a quantidade de DNA do vírus recombinante nos TGs foi 9,7 vezes menor do que do vírus parental, segundo determinação por PCR em tempo real. Assim, a deleção do gene tk (timidina quinase não produziu efeito aparente sobre a frequência da infecção latente, porém reduziu a colonização do TG. Após a

  5. Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters

    NARCIS (Netherlands)

    Vorobyev, Artem; Ujiie, Hideyuki; Recke, Andreas; Buijsrogge, Jacqueline J. A.; Jonkman, Marcel F.; Pas, Hendrikus; Iwata, Hiroaki; Hashimoto, Takashi; Kim, Soo-Chan; Kim, Jong Hoon; Groves, Richard; Samavedam, Unni; Gupta, Yask; Schmidt, Enno; Zillikens, Detlef; Shimizu, Hiroshi; Ludwig, Ralf J.

    Epidermolysis bullosa acquisita (EBA) is an autoimmune blistering disease of the skin and mucous membranes, characterized by autoantibodies against type VII collagen (COL7), a major component of anchoring fibrils. Different clinical EBA phenotypes are described, including mechanobullous and

  6. Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters

    NARCIS (Netherlands)

    Vorobyev, Artem; Ujiie, Hideyuki; Recke, Andreas; Buijsrogge, Jacqueline J. A.; Jonkman, Marcel F.; Pas, Hendrikus; Iwata, Hiroaki; Hashimoto, Takashi; Kim, Soo-Chan; Kim, Jong Hoon; Groves, Richard; Samavedam, Unni; Gupta, Yask; Schmidt, Enno; Zillikens, Detlef; Shimizu, Hiroshi; Ludwig, Ralf J.

    2015-01-01

    Epidermolysis bullosa acquisita (EBA) is an autoimmune blistering disease of the skin and mucous membranes, characterized by autoantibodies against type VII collagen (COL7), a major component of anchoring fibrils. Different clinical EBA phenotypes are described, including mechanobullous and inflamma

  7. Cultura simbolica y fiestas borbonicas en Nueva Granada. De las exequias de Luis I a la proclamacion de Fernando VII

    National Research Council Canada - National Science Library

    Rodriguez Moya, Inmaculada; Minguez Cornelles, Victor

    2012-01-01

    ...: las exequias de Luis I en Santa Fe de Bogota en 1724 y la jura de Fernando VII en San Bartolome de Honda en 1808, centrandonos especialmente en el analisis de las fuentes y la cultura emblematica...

  8. Path-integral simulation of ice VII: Pressure and temperature effects

    CERN Document Server

    Herrero, Carlos P

    2015-01-01

    Effects of pressure and temperature on structural and thermodynamic properties of ice VII have been studied by using path-integral molecular dynamics (PIMD) simulations. Temperatures between 25 and 450 K, as well as pressures up to 12 GPa were considered. Interatomic interactions were modeled by using the effective q-TIP4P/F potential for flexible water. We analyze the pressure dependence of the molar volume, bulk modulus, interatomic distances, kinetic energy, and atomic delocalization at various temperatures. Results of PIMD simulations are compared with those derived from a quasi-harmonic approximation (QHA) of vibrational modes, which helps to assess the importance of anharmonic effects, as well as the influence of the different modes on the properties of ice VII. The accuracy of the QHA for describing this high-pressure phase decreases for rising temperature, but this approximation becomes more reliable as pressure grows, since anharmonicity becomes less relevant. Comparisons with low-pressure cubic ice ...

  9. XMM-Newton discovery of O VII emission from warm gas in clusters of galaxies

    CERN Document Server

    Kaastra, J S; Tamura, T; Paerels, F B S; Den Herder, J W A

    2003-01-01

    XMM-Newton recently discovered O VII line emission from ~2 million K gas near the outer parts of several clusters of galaxies. This emission is attributed to the Warm-Hot Intergalactic Medium. The original sample of clusters studied for this purpose has been extended and two more clusters with a soft X-ray excess have been found. We discuss the physical properties of the warm gas, in particular the density, spatial extent, abundances and temperature.

  10. Redox reactions of Tc{sup VII} with magnetite and mackinawite. Solving an old enigma

    Energy Technology Data Exchange (ETDEWEB)

    Yalcintas, E.; Gaona, X.; Altmeier, M. [Karlsruhe Institute of Technology (KIT), Karlsruhe (Germany). Inst. for Nuclear Waste Disposal; Scheinost, Andreas C. [Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden (Germany). Molecular Structures

    2017-06-01

    While the reduction of heptavalent technetium, Tc(VII), by magnetite and mackinawite under anoxic conditions is well known, the identification of the resulting Tc(IV) species shows substantial disagreement between different studies. Here we use a systematic variation of initial Tc concentration, loading and pH to decipher the Tc species by EXAFS spectroscopy and to shine light on their formation pathways.

  11. Desain Pembelajaran Materi Belah Ketupat Menggunakan Kain Jumputan Palembang untuk Siswa Kelas VII

    Directory of Open Access Journals (Sweden)

    Fauziyah Fauziyah

    2016-06-01

    Full Text Available Desain pembelajaran materi belah ketupat menggunakan kain Jumputan Palembang untuk siswa kelas VII. Penelitian ini bertujuan untuk melihat bagaimana penggunaan kain Jumputan Palembang dalam membangun pemahaman siswa mengenai belah ketupat. Siswa akan membandingkan luas daerah dua buah kain Jumputan, merancang dan membuat belah ketupat kemudian menggunting serta menyusunnya kembali menjadi suatu bentuk baru, menentukan luas daerah belah ketupat, membandingkan keliling dua buah kain Jumputan dan menentukan keliling belah ketupat. Subjek penelitian ini adalah 30 siswa kelas VII SMP Pusri Palembang. Penelitian ini menggunakan design research yang terdiri dari tiga tahap : preparing for the experiment, experiment in the classroom (pilot experiment dan  teaching experiment, dan retrospective analysis. Pada tahap preparing for the experiment, beberapa aktifitas dibuat menggunakan pendekatan Pendidikan Matematika Realistik Indonesia (PMRI. Hasil dari penelitian menunjukkan kain Jumputan Palembang dapat digunakan sebagai starting point dalam pembelajaran belah ketupat untuk siswa kelas VII SMP.The design learning rhombus using kain Jumputan Palembang for VII grade students. This research aims to investigate how to use Kain Jumputan Palembang to build student’s understanding on rhombus. Students were required to comparing the area of two cloths, designing and making rhombus then reshaping to new shapes, determining the area of rhombus, comparing the perimeter of two cloths and determining the perimeter of rhombus. Subjects were 30 first grade students of SMP Pusri Palembang. The study used a design research method consisting of three stages : preparing for the experiment, experiment in the classroom (pilot experiment and teaching experiment, and retrospective analysis. During the preparing for the experiment stage, instructional activities was designing utilizing of Indonesian Realistic Mathematics Education (PMRI approach. The result of experiments

  12. The Architecture of EssB, an Integral Membrane Component of the Type VII Secretion System

    OpenAIRE

    Zoltner, Martin; Norman, David G.; Fyfe, Paul K.; El Mkami, Hassane; Palmer, Tracy; Hunter, William N.

    2013-01-01

    Supported by the Biotechnology and Biological Sciences Research Council (H007571), the Medical Research Council (UK) (G117/519), and the Wellcome Trust (grants 082596, 083481, 094090, and 099149). The membrane-bound EssB is an integral and essential component of the bacterial type VII secretion system that can contribute to pathogenicity. The architecture of Geobacillus thermodenitrificans EssB has been investigated by combining crystallographic and EPR spectroscopic methods. The protein f...

  13. Release of the ENDF/B-VII.1 Evaluated Nuclear Data File

    Energy Technology Data Exchange (ETDEWEB)

    Brown, David

    2012-06-30

    The Cross Section Evaluation Working Group (CSEWG) released the ENDF/B-VII.1 library on December 22, 2011. The ENDF/B-VII.1 library is CSEWG's latest recommended evaluated nuclear data file for use in nuclear science and technology applications, and incorporates advances made in the five years since the release of ENDF/B-VII.0, including: many new evaluation in the neutron sublibrary (423 in all and over 190 of these contain covariances), new fission product yields and a greatly improved decay data sublibrary. This summary barely touches on the five years worth of advances present in the ENDF/B-VII.1 library. We expect that these changes will lead to improved integral performance in reactors and other applications. Furthermore, the expansion of covariance data in this release will allow for better uncertainty quantification, reducing design margins and costs. The ENDF library is an ongoing and evolving effort. Currently, the ENDF data community embarking on several parallel efforts to improve library management: (1) The adoption of a continuous integration system to provide evaluators 'instant' feedback on the quality of their evaluations and to provide data users with working 'beta' quality libraries in between major releases. (2) The transition to new hierarchical data format - the Generalized Nuclear Data (GND) format. We expect GND to enable new kinds of evaluated data which cannot be accommodated in the legacy ENDF format. (3) The development of data assimilation and uncertainty propagation techniques to enable the consistent use of integral experimental data in the evaluation process.

  14. Laparoscopic Liver Resection Using the Lateral Approach from Intercostal Ports in Segments VI, VII, and VIII.

    Science.gov (United States)

    Inoue, Yoshihiro; Suzuki, Yusuke; Fujii, Kensuke; Kawaguchi, Nao; Ishii, Masatsugu; Masubuchi, Shinsuke; Yamamoto, Masashi; Hirokawa, Fumitoshi; Hayashi, Michihiro; Uchiyama, Kazuhisa

    2017-07-31

    Laparoscopic liver resection (LLR) has been developed as a minimally invasive surgery. However, challenges such as difficulty securing visibility and limited control of forceps make it difficult to complete LLR in hepatic segments VI, VII, and VIII. To overcome these challenges, we devised a surgical technique using intercostal ports. We termed this approach the lateral approach. This work describes our experience performing LLR using this approach and discusses the safety and effectiveness of this approach. Between April 2011 and December 2016, data from 91 patients who underwent LLR with or without the intercostal port at a single institution were retrospectively analyzed regarding surgical outcomes, safety, and utility. LLR was performed for 32 patients with the intercostal port and for 59 patients without the intercostal port. The conversion rates to open surgery with and without intercostal ports were 3.1 and 25.4% (P = 0.008). In hepatic segments VII and VIII, the rates of conversion to open surgery were significantly lower for cases involving intercostal ports (6.7 vs. 42.9 and 0 vs. 38.9%; P = 0.035 and 0026, respectively); however, there were no differences in hepatic segment VI (0 vs. 7.4%; P = 0.563). There were no differences in operative time, blood loss volume, surgical margin, curative resection rate, or postoperative complication rate for LLR in all segments (VI, VII, and VIII). No adverse events due to placement of the intercostal port were observed in this set of patients. LLR using the lateral approach and intercostal ports for hepatic segments VII and VIII resulted in a significant decrease in conversion rates to open surgery.

  15. Environmental evaluation of the PIES Trendlong Mid-Mid Scenario: Federal Region VII

    Energy Technology Data Exchange (ETDEWEB)

    1979-07-01

    Findings of the environmental impact evaluation of the PIES TRENDLONG MID-MID Scenario for Federal Region VII (Iowa, Kansas, Nebraska, and Missouri) are reported. Projected coal-fired utility expansion could be constrained in several areas because of projected TSP and SO/sub 2/ National Ambient Air Quality Standards (NAAQS) violations. Problems will be most pronounced in Iowa and Kansas, but could be incurred in any state in the region. Large coal and nuclear facilities projected for parts of Region VII may experience water availability problems. The Smokey Hill River in Kansas, the Skunk River in Iowa, and the Platte River in Nebraska may have limited water supplies for operating the projected increases in fossil fuel and nuclear generating facilities. Coal surface mining in Missouri (21,000 acres may be disturbed from 1975 to 1990) may create land-use conflicts. Region VII may not have the facilities or legal framework for mitigating the negative socioeconomic impacts that are projected to occur from the proposed energy development. Therefore, the potential for severe and pervasive socioeconomic impacts is great.

  16. VII Zw 403: HI Structure in a Blue Compact Dwarf Galaxy

    CERN Document Server

    Simpson, Caroline E; Nordgren, Tyler E; Brinks, Elias; Elmegreen, Bruce G; Ashley, Trisha; Lynds, Roger; McIntyre, Vince J; O'Neil, Earl J; Östlin, Göran; Westpfahl, David J; Wilcots, Eric M

    2011-01-01

    We present optical (UBVJ), ultraviolet (FUV, NUV), and high resolution atomic hydrogen (HI) observations of the nearby blue compact dwarf (BCD), VII Zw 403. We find that VII Zw 403 has a relatively high HI mass-to-light ratio for a BCD. The rotation velocity is nominally 10-15 km/s, but rises to ~20 km/s after correction for the ~8-10 km/s random motions present in the gas. The velocity field is complex; including a variation in the position angle of the major axis going from the NE to the SW parts of the galaxy. Our high resolution HI maps reveal structure in the central gas, including a large, low-density HI depression or hole between the southern and northern halves of the galaxy, coincident with an unresolved x-ray source. Although interactions have been proposed as the triggering mechanism for the vigorous star formation occurring in BCDs, VII Zw 403 does not seem to have been tidally triggered by an external interaction, as we have found no nearby possible perturbers. It also doesn't appear to fall in t...

  17. Immobilization of 99-Technetium (VII) by Fe(II)-Goethite and Limited Reoxidation

    Energy Technology Data Exchange (ETDEWEB)

    W Um; H Chang; J Icenhower; W Lukens; R Serne; N Qafoku; J Westsik Jr.; E Buck; S Smith

    2011-12-31

    During the nuclear waste vitrification process volatilized {sup 99}Tc will be trapped by melter off-gas scrubbers and then washed out into caustic solutions, and plans are currently being contemplated for the disposal of such secondary waste. Solutions containing pertechnetate [{sup 99}Tc(VII)O{sub 4}{sup -}] were mixed with precipitating goethite and dissolved Fe(II) to determine if an iron (oxy)hydroxide-based waste form can reduce Tc(VII) and isolate Tc(IV) from oxygen. The results of these experiments demonstrate that Fe(II) with goethite efficiently catalyzes the reduction of technetium in deionized water and complex solutions that mimic the chemical composition of caustic waste scrubber media. Identification of the phases, goethite + magnetite, was performed using XRD, SEM and TEM methods. Analyses of the Tc-bearing solid products by XAFS indicate that all of the Tc(VII) was reduced to Tc(IV) and that the latter is incorporated into goethite or magnetite as octahedral Tc(IV). Batch dissolution experiments, conducted under ambient oxidizing conditions for more than 180 days, demonstrated a very limited release of Tc to solution (2-7 {micro}g Tc/g solid). Incorporation of Tc(IV) into the goethite lattice thus provides significant advantages for limiting reoxidation and curtailing release of Tc disposed in nuclear waste repositories.

  18. MENDF71x. Multigroup Neutron Cross Section Data Tables Based upon ENDF/B-VII.1

    Energy Technology Data Exchange (ETDEWEB)

    Conlin, Jeremy Lloyd [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Parsons, Donald Kent [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Gardiner, Steven J. [Univ. of California, Davis, CA (United States); Gray, Mark Girard [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Lee, Mary Beth [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); White, Morgan Curtis [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-12-17

    A new multi-group neutron cross section library has been released along with the release of NDI version 2.0.20. The library is named MENDF71x and is based upon the evaluations released in ENDF/B-VII.1 which was made publicly available in December 2011. ENDF/B-VII.1 consists of 423 evaluations of which ten are excited states evaluations and 413 are ground state evaluations. MENDF71x was created by processing the 423 evaluations into 618-group, downscatter only NDI data tables. The ENDF/B evaluation files were processed using NJOY version 99.393 with the exception of 35Cl and 233U. Those two isotopes had unique properties that required that we process the evaluation using NJOY version 2012. The MENDF71x library was only processed to room temperature, i.e., 293.6 K. In the future, we plan on producing a multi-temperature library based on ENDF/B-VII.1 and compatible with MENDF71x.

  19. MERLIN and VLA Observations of VII Zw 19: Distant Cousin of M82

    CERN Document Server

    Beck, S C; Turner, J L; Van Dyk, S D

    2004-01-01

    We have observed the starburst dwarf galaxy VII Zw 19 at subarcsecond resolution with the MERLIN and MERLIN+EVN networks at 18 cm, with MERLIN at 6 cm, and with the VLA in A array at 6 and 2 cm. The galaxy is detected at all VLA wavelengths and is resolved. It is also resolved at 18 cm by MERLIN, but is not detected by the EVN nor by MERLIN at 6 cm. VII Zw 19 has a complex structure of nonthermal radio emission at 18 cm extended over ~1200-1800 pc (4-6''). That the EVN did not detect this emission indicates that there are no obvious point sources that could be radio supernovae. The 2 cm emission, predominantly thermal free-free emission, has a markedly different spatial distribution from the nonthermal emission. The radio colors show that the galaxy contains numerous supernova remnants, as well as many young HII regions, many of which are optically thick at 6 cm. Two extended regions of 2 cm emission have little 18 cm flux and are probably emission from young star clusters. VII Zw 19 resembles M82 in its radi...

  20. Revealing O VII from stacked X-ray grating spectra of clusters, groups and elliptical galaxies

    CERN Document Server

    Sanders, J S

    2010-01-01

    We stack 4.6 Ms of high spectral resolution XMM-Newton Reflection Grating Spectrometer spectra from galaxy clusters, groups of galaxies and elliptical galaxies. For those objects with a central temperature of less than 1 keV, we detect O VII for the first time, with a probability of false detection of 2.5x10^-4. The flux ratio of the O VII to Fe XVII lines is 1/4 to 1/8 of the emission expected for isobaric radiative cooling in the absence of heating. There is either a process preventing cooling below 0.5 keV, anomalous O/Fe abundance ratios, absorbing material around the coolest X-ray emitting gas or non-radiative cooling taking place. The mean N VII emission line is strong in the sub-keV sample. As the ratio of the hydrogenic N and O lines is largely independent of temperature, we measure a mean N/O ratio of 4.0 +- 0.6 Solar. Although the continuum around the C VI lines is difficult to measure we can similarly estimate that the C/O ratio is 0.9 +- 0.3 Solar.

  1. Equations of state of ice VI and ice VII at high pressure and high temperature.

    Science.gov (United States)

    Bezacier, Lucile; Journaux, Baptiste; Perrillat, Jean-Philippe; Cardon, Hervé; Hanfland, Michael; Daniel, Isabelle

    2014-09-14

    High-pressure H2O polymorphs among which ice VI and ice VII are abundant in the interiors of large icy satellites and exo-planets. Knowledge of the elastic properties of these pure H2O ices at high-temperature and high-pressure is thus crucial to decipher the internal structure of icy bodies. In this study we assess for the first time the pressure-volume-temperature (PVT) relations of both polycrystalline pure ice VI and ice VII at high pressures and temperatures from 1 to 9 GPa and 300 to 450 K, respectively, by using in situ synchrotron X-ray diffraction. The PVT data are adjusted to a second-order Birch-Murnaghan equation of state and give V0 = 14.17(2) cm(3) mol(-1), K0 = 14.05(23) GPa, and α0 = 14.6(14) × 10(-5) K(-1) for ice VI and V0 = 12.49(1) cm(3) mol(-1), K0 = 20.15(16) GPa, and α0 = 11.6(5) × 10(-5) K(-1) for ice VII.

  2. Application of Corn Cob as a Natural Adsorbent for the Removal of Mn (Vii Ions from Aqueous Solutions

    Directory of Open Access Journals (Sweden)

    Fariba Norozi

    2016-08-01

    Full Text Available In this Work, We have studied the adsorption of the isotherm of Mn(VII, on Corn cob from aqueous solutions. The effects of pH, initial metal ion concentration, contact time, Corn cobdosage and temperature on the adsorption performance of Corn cob for Mn(VII ions were examined by batch method. Increasing the Mn(VII initial concentration declines the Mn(VII adsorption rate investigated through adsorption Mn(VII removal at pH=4 condition for tC=60 min in equilibrium-batch mode system. The adsorption equilibrium isotherms were fitted by Freundlich and Langmuir models. It was found that the Langmuir model described the adsorption process better than the Freundlich isotherm,(R2=0.9854, thus indicating the applicability of mono layer coverage of Mn(VII ion on Corn cob surface.The relationship between thermodynamic parameters was used to predict the absorption process. Thermodynamic analysis showed that the adsorption process was endothermic and spontaneous in nature.

  3. A high-fat meal does not activate blood coagulation factor VII in minipigs

    DEFF Research Database (Denmark)

    Olsen, A K; Larsen, L F; Bladbjerg, E-M;

    2001-01-01

    , 3.5, 4, 5, and 6 h after the first fat load. Triglycerides, activated FVII (FVIIa), FVII coagulant activity (FVIIc), FVII amidolytic activity (FVIIam) and prothrombin fragment I + 2 (F1 + 2) were analysed in plasma samples. Median plasma triglycerides were significantly raised from 0.67 mmol....../l (baseline) to 2.56 mmol/l 5 h postprandially (P high-fat meal does not seem...

  4. Efficacy and safety of recombinant activated factor VII for acute intracerebral hemorrhage

    DEFF Research Database (Denmark)

    Mayer, Stephan A; Brun, Nikolai C; Begtrup, Kamilla;

    2008-01-01

    patients with intracerebral hemorrhage to receive placebo (268 patients), 20 microg of rFVIIa per kilogram of body weight (276 patients), or 80 microg of rFVIIa per kilogram (297 patients) within 4 hours after the onset of stroke. The primary end point was poor outcome, defined as severe disability...... or death according to the modified Rankin scale 90 days after the stroke. RESULTS: Treatment with 80 microg of rFVIIa per kilogram resulted in a significant reduction in growth in volume of the hemorrhage. The mean estimated increase in volume of the intracerebral hemorrhage at 24 hours was 26......% in the placebo group, as compared with 18% in the group receiving 20 microg of rFVIIa per kilogram (P=0.09) and 11% in the group receiving 80 microg (P

  5. Effects of dietary fat quality and quantity on postprandial activation of blood coagulation factor VII

    DEFF Research Database (Denmark)

    Larsen, L F; Bladbjerg, E-M; Jespersen, J

    1997-01-01

    , monounsaturated, or polyunsaturated fats differed regarding postprandial activation of FVII. Eighteen healthy young men participated in the study. On 6 separate days each participant consumed two meals (times, 0 and 1 3/4 hours) enriched with 70 g (15 and 55 g) of either rapeseed oil, olive oil, sunflower oil......, palm oil, or butter (42% of energy from fat) or isoenergetic low-fat meals (6% of energy from fat). Fasting and series of nonfasting blood samples (the last at time 8 1/2 hours) were collected. Plasma triglycerides, FVIIc, FVIIa, and free fatty acids were analyzed. There were marked effects of the fat...

  6. Plasma concentrations of blood coagulation factor VII measured by immunochemical and amidolytic methods

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Gram, J; Jespersen, J

    2000-01-01

    plasma, in cold activated plasma and in FVII deficient plasma. There was a positive correlation (r=0.96) between FVII:Ag and FVII:Am with slightly but significantly higher values for FVII:Ag (FVII:Ag= 106 U/ml and FVII:Am=100 U/ml; p ... omitting the data from patients on oral anticoagulant therapy, with mean values of 113 U/ml for FVII:Ag and 110 U/ml for FVII:Am (p

  7. A high fat meal activates blood coagulation factor VII in rats

    DEFF Research Database (Denmark)

    Olsen, Aage K; Bladbjerg, Else M; Hansen, Axel K

    2002-01-01

    the LEW/Mol rat. We gavaged 3 mL of a fat emulsion (n = 42) or 3 mL isotonic glucose (n = 42). Blood was sampled by heart puncture 2, 4 and 6 h (n = 14/group at each time) after the fat/glucose load. Furthermore, blood was sampled from 16 untreated rats to determine the baseline levels. Triglyceride...

  8. Photocontrol of Anthocyanin Synthesis: VII. Factors Affecting the Spectral Sensitivity of Anthocyanin Synthesis in Young Seedlings.

    Science.gov (United States)

    Mancinelli, A L; Walsh, L

    1979-05-01

    Light-dependent anthocyanin synthesis is a typical high irradiance response (HIR) of plant photomorphogenesis. The spectral sensitivity of this response in young seedlings of cabbage and tomato is strongly affected by the length and mode of application of the light treatments. This observation suggests that the different experimental conditions, used in different action spectroscopy studies, might have been responsible, at least in part, for some of the reported differences in the characteristics of the HIR action spectra of different response-system combinations. In both cabbage and tomato, the values of the far red/blue, far red/red, and blue/red action ratios increase with increasing durations of the light treatments; this finding is in agreement with hypotheses of K. M. Hartmann (1966, 1967) and E. Schäfer (1975) for phytochrome action in the HIR. The similarity in the trend of change of the values of the action ratios suggests the possibility that the photomorphogenic pigment system, involved in the photoregulation of anthocyanin synthesis, may be the same in cabbage and tomato, even though there are some differences in the spectral sensitivity of the response between the two species.

  9. Contribution of coagulation factor VII R353Q polymorphism to the ...

    African Journals Online (AJOL)

    Hanan Azzam

    2016-12-05

    Dec 5, 2016 ... also modulate its response to environmental stimuli [6]. Thus, they could ... work was carried out in accordance with The Code of Ethics of the World ... and then the 312 bp was digested with Msp1 restriction enzyme. (Thermo ...

  10. Effects of dietary fat quality and quantity on postprandial activation of blood coagulation factor VII

    DEFF Research Database (Denmark)

    Larsen, L F; Bladbjerg, E-M; Jespersen, J

    1997-01-01

    , palm oil, or butter (42% of energy from fat) or isoenergetic low-fat meals (6% of energy from fat). Fasting and series of nonfasting blood samples (the last at time 8 1/2 hours) were collected. Plasma triglycerides, FVIIc, FVIIa, and free fatty acids were analyzed. There were marked effects of the fat...... quantity on postprandial responses of plasma triglycerides, FVII, and free fatty acids. The high-fat meals caused, in contrast to the low-fat meals, considerable increases in plasma triglycerides. Plasma levels of FVIIc and FVIIa peaks were 7% and 60% higher after consumption of high-fat meals than after...... consumption of low-fat meals. The five different fat qualities caused similar postprandial increases in plasma triglycerides, FVIIc, and FVIIa. These findings indicate that high-fat meals may be prothrombotic, irrespective of their fatty acid composition. The postprandial FVII activation was not associated...

  11. Factor VII-activating protease: Mechanism and regulation of nucleosome release from dead cells

    NARCIS (Netherlands)

    F. Stephan

    2014-01-01

    Cell death is indispensable for tissue homeostasis and is a fundamental principle of inflammation and lymphocyte generation. Rapid removal and destruction of cellular corpses is critical and insufficient removal may lead to the release of cellular content into the environment. These immunogenic "hos

  12. Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System

    Directory of Open Access Journals (Sweden)

    Sina Mirzaahmadi

    2011-01-01

    Full Text Available The variety of recombinant protein expression systems have been developed as a resource of FVII gene expression. In the current study, the authors used a novel protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host for expression of FVII. Plasmid containing cDNA encoding full-length human FVII was introduced into Lizard Leishmania and positive transfectants were analyzed by SDS-PAGE and Western blot analysis. Furthermore, biological activity of purified protein was detected by PT assay. The recombinant strain harboring a construct was analyzed for expression of FVII at the mRNA and protein level. Purified rFVII was obtained and in order to confirm the purified compound was in fact rFVII. Western blot analysis was carried out. Clotting time in PT assay was reduced about 30 seconds with the purified rFVII. In Conclusion, this study has demonstrated, for the first time, that Leishmania cells can be used as an expression system for producing recombinant FVII.

  13. Exonuclease VII is involved in "reckless" DNA degradation in UV-irradiated Escherichia coli.

    Science.gov (United States)

    Repar, Jelena; Briški, Nina; Buljubašić, Maja; Zahradka, Ksenija; Zahradka, Davor

    2013-01-20

    The recA mutants of Escherichia coli exhibit an abnormal DNA degradation that starts at sites of double-strand DNA breaks (DSBs), and is mediated by RecBCD exonuclease (ExoV). This "reckless" DNA degradation occurs spontaneously in exponentially growing recA cells, and is stimulated by DNA-damaging agents. We have previously found that the xonA and sbcD mutations, which inactivate exonuclease I (ExoI) and SbcCD nuclease, respectively, markedly suppress "reckless" DNA degradation in UV-irradiated recA cells. In the present work, we show that inactivation of exonuclease VII (ExoVII) by an xseA mutation contributes to attenuation of DNA degradation in UV-irradiated recA mutants. The xseA mutation itself has only a weak effect, however, it acts synergistically with the xonA or sbcD mutations in suppressing "reckless" DNA degradation. The quadruple xseA xonA sbcD recA mutants show no sign of DNA degradation during post-irradiation incubation, suggesting that ExoVII, together with ExoI and SbcCD, plays a crucial role in regulating RecBCD-catalyzed chromosome degradation. We propose that these nucleases act on DSBs to create blunt DNA ends, the preferred substrates for the RecBCD enzyme. In addition, our results show that in UV-irradiated recF recA(+) cells, the xseA, xonA, and sbcD mutations do not affect RecBCD-mediated DNA repair, suggesting that ExoVII, ExoI and SbcCD nucleases are not essential for the initial targeting of RecBCD to DSBs. It is possible that the DNA-blunting activity provided by ExoVII, ExoI and SbcCD is required for an exchange of RecBCD molecules on dsDNA ends during ongoing "reckless" DNA degradation.

  14. Contamination monitoring and control on ASML MS-VII 157-nm exposure tool

    Science.gov (United States)

    Okoroanyanwu, Uzodinma; Gronheid, Roel; Coenen, Jan; Hermans, Jan; Ronse, Kurt G.

    2004-05-01

    This paper presents results of monitoring and control of contaminants in an ASML MS-VII 157nm full-field exposure tool at IMEC, as verified lithographically in terms of field uniformity, lens transmission, CD uniformity, and scattered light. The daily contamination monitoring system utilizes in-line photo-ionization detector, oxygen and moisture analyzers, as well as chemiluminescent detector, and gas chromatograph that is coupled to a mass spectrometer. On a monthly basis, contamination monitoring was performed with thermal desorption-gas chromatographi/mass spectrometric techniques. The following four locations within the optical path of the MS-VII are monitored: source optic assembly, condenser lens optic, 1X relay station, and projection optics box. Contamination control is realized in the system with an on-board purge control unit, which is equipped with gas purifiers that remove contaminants such as H2O, O2, CO, CO2, hydrocarbons, H2, and sulfur compounds. All the observed contaminants have been trending within expected values and no contamination-related tool performance degradation has been observed. The excursions observed in the contaminant concentrations are coincident with tool downtime/maintenance events. Siloxane levels appear to be consistently below 50 ppt in all the monitored locations within the optical path of the tool, except on one occasion when it reached 90 ppt in the projection optics. Volatile organic compounds (VOCs) concentration within the MS-VII enclosure show a stable background level of around 10-25 ppb during weekends and levels of 45-60 ppb (during working days). VOCs concentration variations inside the MS-VII enclosure during the working days correlate well with activities inside the clean room. Air recirculation and low intake of fresh air inside the MS-VII tend to slow down the speed with which the VOCs levels decreases to stable background level, whenever there was a major upward excursion in their concentration. Average light

  15. Dinâmica folicular em cabras da raça Toggenburg em lactação tratadas ou não com somatotropina bovina recombinante Follicular dynamics of lactating Toggenburg does treated with recombinant bovine somatotropin

    Directory of Open Access Journals (Sweden)

    E.A.M. Amorim

    2007-12-01

    Full Text Available Avaliou-se o efeito da injeção de somatotropina bovina recombinante (r-bST sobre o ciclo estral de cabras. Foram utilizadas 24 fêmeas da raça Toggenburg, distribuídas em dois tratamentos: T1 (n=12, cabras tratadas com r-bST; e T2 (n=12, cabras tratadas com solução salina (controle. A partir da primeira injeção do tratamento, acompanhou-se o ciclo dos animais em estro até o estro subseqüente. O comprimento médio do ciclo estral e o período interovulatório não diferiram (P>0,05 entre os tratamentos. Foram verificados ciclos estrais de duas, três e quatro ondas de crescimento folicular. Não houve diferença (P>0,05 entre os tratamentos quanto ao número de ondas do ciclo estral. O número de folículos detectados com diâmetro >3mm nas cabras do T1 foi maior (P0,05. A r-bST não alterou a dinâmica folicular de cabras lactantes da raça Toggenburg, mas promoveu o aumento do número de folículos recrutados ³3mm durante o ciclo estral.The effect of the r-bST injection was evaluated on the goats estrous cycles. Twenty-four Toggenburg does were used in two treatments: T1 (n=12 treated with r-bST; and T2 (n=12 treated with saline solution (control. After the first r-bST injection, the estrous cycles were checked between two estrus. The estrous cycle length and interovulatory period of the goats did not differ between treatments (P>0.05. Estrous cycles with two, three and four waves of follicular growth were observed. The number of waves during the estrous cycle were not affected by the r-bST treatment (P>0.05. The number of 3mm follicles was different between T1 and T2 (P0.05 between treatments. The r-bST did not affect the ovarian response during the estrous cycle. The r-bST not affected the follicular dynamic of Toggenburg lactating does, but increased the number of emerging follicles >3mm during the estrous cycle.

  16. Caracterização da imunogenicidade das proteínas recombinantes Virb9, Virb10 e fator termo instável de Elongação de Peptídeos de Anaplasma marginale em camundongos

    Directory of Open Access Journals (Sweden)

    Ana Beatriz Canevari Castelão

    2012-10-01

    Full Text Available Considerando as limitações dos atuais métodos de controle contra a anaplasmose bovina, o desenvolvimento de uma vacina efetiva se faz necessário. A partir do advento da análise genômica e proteômica, novas proteínas de membrana de Anaplasma marginale foram identificadas como possíveis candidatas a componentes de uma vacina, tais como, VirB9,VirB10 e Fator Termo Instavél de Elongação de Peptídeos (EF-Tu. Embora estas proteínas ainda não estejam bem caracterizadas na membrana de A. marginale, a produção destas na forma recombinante (rVirB 9, rVirB10 e rEF-Tutem sido realizada, mas as mesmas ainda não foram exploradas em formulações vacinais. Neste trabalho, avaliou-se ouso de rVirB9, rVirB10 e rEF-Tu emulsionadas em adjuvante Montanide em camundongos. Nas condições testadas, verificou-se a indução de forte resposta imune humoral com a produção de IgG1 e IgG2a, sendo que as proporções dos níveis de produção destas subclasses indicam predomínio de IgG1. Entretanto, esplenócitos de animais, que foram injetados com rVirB9 ou rVirB10, produziram interferon-gama acima do limite de detecção do ensaio após estimulação in vitro, sinalizando assim resposta celular específica. Assim, novas avaliações serão realizadas com a finalidade de modular o perfil de resposta imune obtido em bovinos e avaliar a proteção contra A. marginale.

  17. Serum zinc and hormonal profile in male dialysis patients receiving human recombinant erythropoietin Zinco sérico e perfil hormonal de pacientes do sexo masculino submetidos à hemodiálise em uso de eritropoetina humana recombinante

    Directory of Open Access Journals (Sweden)

    Maria Mouranilda Schleicher

    2005-08-01

    Full Text Available INTRODUCTION: Treatment with recombinant human erythropoietin (rHuEpo is associated with an improvement in well-being and quality of life in patients submitted to maintenance hemodialysis (HD. OBJECTIVES: The goal of this work was to evaluate the levels of sex hormones, hematocrit, albumin and zinc in HD patients with rHuEpo therapy and compare them with those observed in patients without rHuEpo treatment. MATERIAL AND METHODS: Two groups of twelve male HD patients each were selected for a transversal study; one did not receive rHuEpo (group 1 whereas the other one did (group 2. Levels of hematocrit, albumin, zinc, luteinizing hormone (LH, follicle-stimulating hormone (FSH, prolactin, and testosterone were determined. RESULTS: Group 2 patients showed significantly higher medians (p INTRODUÇÃO: Em pacientes submetidos à hemodiálise crônica (HD, o tratamento com eritropoetina humana recombinante (rHuEpo está associado a melhora no bem-estar geral e na qualidade de vida. OBJETIVOS: O objetivo do presente trabalho foi avaliar os níveis dos hormônios sexuais e do zinco em pacientes sob HD e em uso de rHuEpo em comparação com pacientes sem tratamento com essa droga. MATERIAL E MÉTODOS: Dois grupos de doze pacientes do sexo masculino cada um, submetidos à HD, sendo um deles sem uso de rHuEpo (grupo 1 e o outro utilizando a droga (grupo 2, foram selecionados para um estudo transversal, comparando-se os níveis séricos do zinco, da albumina, dos hormônios FSH, LH, prolactina, testosterona e do hematócrito. RESULTADOS: No grupo 2, os valores de testosterona (4,65 vs. 3,5ng/ml, hematócrito (30,5 vs. 22%, albumina (3,9 vs. 3,7g/dl e zinco (62,5 vs. 50,5microg/dl foram significativamente maiores do que no grupo 1 (p < 0,05. DISCUSSÃO: Sugere-se que, em pacientes recipientes da rHuEpo, os níveis mais altos de hematócrito, zinco, albumina e testosterona possam ser fatores que contribuam para melhorar a disfunção sexual e a qualidade de

  18. Combination of recombinant factor VIIa and fibrinogen corrects clot formation in primary immune thrombocytopenia at very low platelet counts

    DEFF Research Database (Denmark)

    Larsen, Ole H; Stentoft, Jesper; Radia, Deepti

    2013-01-01

    Haemostatic treatment modalities alternative to platelet transfusion are desirable to control serious acute bleeds in primary immune thrombocytopenia (ITP). This study challenged the hypothesis that recombinant activated factor VII (rFVIIa) combined with fibrinogen concentrate may correct whole b...

  19. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism.

    Science.gov (United States)

    Solomonson, Matthew; Setiaputra, Dheva; Makepeace, Karl A T; Lameignere, Emilie; Petrotchenko, Evgeniy V; Conrady, Deborah G; Bergeron, Julien R; Vuckovic, Marija; DiMaio, Frank; Borchers, Christoph H; Yip, Calvin K; Strynadka, Natalie C J

    2015-03-03

    Mycobacterium tuberculosis (Mtb) uses the ESX-1 type VII secretion system to export virulence proteins across its lipid-rich cell wall, which helps permeabilize the host's macrophage phagosomal membrane, facilitating the escape and cell-to-cell spread of Mtb. ESX-1 membranolytic activity depends on a set of specialized secreted Esp proteins, the structure and specific roles of which are not currently understood. Here, we report the X-ray and electron microscopic structures of the ESX-1-secreted EspB. We demonstrate that EspB adopts a PE/PPE-like fold that mediates oligomerization with apparent heptameric symmetry, generating a barrel-shaped structure with a central pore that we propose contributes to the macrophage killing functions of EspB. Our structural data also reveal unexpected direct interactions between the EspB bipartite secretion signal sequence elements that form a unified aromatic surface. These findings provide insight into how specialized proteins encoded within the ESX-1 locus are targeted for secretion, and for the first time indicate an oligomerization-dependent role for Esp virulence factors.

  20. Assessing the Subject General Medicine VII in the University Polyclinic Evaluación de la asignatura Medicina General Integral VII en el policlínico universitario

    Directory of Open Access Journals (Sweden)

    Cynthia Olivia Morales Pérez

    2012-06-01

    Full Text Available Background: the subject General Medicine VII is part of the guiding discipline in Medicine studies. It ensures that when Medicine Doctors obtain their degrees they have acquired all the knowledge that allows them to perform multiple tasks in health care services. Objective: to assess the subject General Medicine VII, in the university polyclinic as part of the educational process. Methods: we conducted a mixed research using quantitative and qualitative methods through a methodology that included the level of satisfaction of students, teachers, managers, and knowledge levels obtained by students. This methodology was applied in the fourth year of Medicine studies and in the four university polyclinics in the municipality of Cienfuegos, inserted into the new training model. Results: managers referred insufficient chances for exchanging with subordinates as well as for the profits of methodological activities, teachers were dissatisfied because of insufficient educational preparation and high work load and students reported difficulties with the availability and on-time delivery of textbooks, in addition to not having the necessary instrumentation for skills appropriation. Conclusions: we found that managers were dissatisfied with organizational and functional aspects, teachers with planning and implementation of the teaching process and students with the literature and the necessary tools while in teaching practice.Fundamento: la asignatura Medicina General Integral VII es parte de la disciplina rectora de la carrera de Medicina, que garantiza que una vez graduado el Médico General, posea conocimientos que le permitan desempeñar múltiples tareas en los servicios de atención médica. Objetivo: evaluar la asignatura Medicina General Integral VII en el policlínico universitario como parte del proceso docente educativo.

  1. Late results of catheter-directed recombinant tissue plasminogen activator fibrinolytic therapy of iliofemoral deep venous thrombosis Resultados de longo prazo do tratamento fibrinolítico da trombose venosa iliacofemoral por infusão seletiva do ativador de plasminogênio tissular recombinante em baixas doses

    Directory of Open Access Journals (Sweden)

    Ivan Benaduce Casella

    2007-02-01

    Full Text Available PURPOSE: To evaluate the efficacy of catheter-directed low-dose recombinant tissue-type plasminogen activator infusion in the treatment of iliofemoral deep venous thrombosis and prevention of post-thrombotic syndrome. METHOD: Eighteen patients (out of 260 evaluated with acute iliofemoral deep venous thrombosis and no previous evidence of venous insufficiency were prospectively selected for thrombolytic therapy. Catheter-directed low-dose recombinant tissue-type plasminogen activator (1 mg/h was infused into the thrombotic segments. RESULTS: Effective fibrinolysis was achieved in 14 of 18 cases, with correlation between effective fibrinolysis and major/complete resolution of acute signs and symptoms (P OBJETIVOS: Avaliar a eficácia da infusão seletiva por cateter do ativador de plasminogênio tecidual recombinante em baixas doses no tratamento da trombose venosa iliacofemoral e na prevenção da síndrome pós-trombótica. MÉTODO: Dezoito pacientes (de 260 avaliados portadores de trombose venosa profunda iliacofemoral sem evidência prévia de insuficiência venosa foram selecionados para terapia fibrinolítica e submetidos a infusão seletiva por cateter do ativador de plasminogênio tecidual recombinante na dose de 1mg/dl nos segmentos venosos trombóticos. RESULTADOS: Quatorze pacientes apresentaram fibrinólise efetiva; observamos correlação entre o grau de melhora clínica observado e a redução percentual do volume trombótico (P<.01. Não houve episódios de complicações graves. Quatro pacientes apresentaram retrombose precoce (1 a 8 semanas. Os pacientes foram seguidos por um período de até 131 semanas (média 85.2. A incidência de sinais e sintomas clínicos de insuficiência venosa e os achados ecográficos de refluxo valvular foram significativamente menores nos pacientes em que a terapia fibrinolítica foi efetiva e a perviedade mantida ao longo do período de seguimento, na comparação com os casos de falha aguda ou de

  2. A novel deletion and two recurrent substitutions on type VII collagen gene in seven Iranian patients with epidermolysis bullosa.

    Science.gov (United States)

    Hamidi, Armita Kakavand; Moghaddam, Mohammad; Hatamnejadian, Nasim; Ebrahimi, Ahmad

    2016-08-01

    Epidermolysis bullosa is one of the most important series of mechano-bullous heritable skin disorders which is categorized into four major types according to the layer that bullae forms within basement membrane zone. In dystrophic form of the disease, blisters are made in the sublamina densa zone, at the level of type VII collagen protein which produce anchoring fibrils. Type VII collagen gene is the only responsible gene for this form. The aim of this study was to survey causative mutations of type VII collagen gene among Iranian patients with epidermolysis bullosa. For this purpose, exons 73-75 were investigated by polymerase chain reaction followed by direct sequencing. In current study, we found three different point mutations in type VII collagen alleles in 7 out of 50 patients. Four patients were homozygous for a new deletion which resulted in frame shift (p.Pro2089fs). Two patients were homozygous for a recurrent glycine substitution (p.G2031S) and one patient was detected with an allele carrying a substitution (p.R2069C). The results emphasized heterogeneity in the type VII collagen gene and will provide a sign for early diagnosis and future study of the disease pathogenesis.

  3. Optimizing Cr(VI) and Tc(VII) remediation through nano-scale biomineral engineering

    Energy Technology Data Exchange (ETDEWEB)

    Cutting, R. S.; Coker, V. S.; Telling, N. D.; Kimber, R. L.; Pearce, C. I.; Ellis, B.; Lawson, R; van der Laan, G.; Pattrick, R.A.D.; Vaughan, D.J.; Arenholz, E.; Lloyd, J. R.

    2009-09-09

    To optimize the production of biomagnetite for the bioremediation of metal oxyanion contaminated waters, the reduction of aqueous Cr(VI) to Cr(III) by two biogenic magnetites and a synthetic magnetite was evaluated under batch and continuous flow conditions. Results indicate that nano-scale biogenic magnetite produced by incubating synthetic schwertmannite powder in cell suspensions of Geobacter sulfurreducens is more efficient at reducing Cr(VI) than either biogenic nano-magnetite produced from a suspension of ferrihydrite 'gel' or synthetic nano-scale Fe{sub 3}O{sub 4} powder. Although X-ray Photoelectron Spectroscopy (XPS) measurements obtained from post-exposure magnetite samples reveal that both Cr(III) and Cr(VI) are associated with nanoparticle surfaces, X-ray Magnetic Circular Dichroism (XMCD) studies indicate that some Cr(III) has replaced octahedrally coordinated Fe in the lattice of the magnetite. Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) measurements of total aqueous Cr in the associated solution phase indicated that, although the majority of Cr(III) was incorporated within or adsorbed to the magnetite samples, a proportion ({approx}10-15 %) was released back into solution. Studies of Tc(VII) uptake by magnetites produced via the different synthesis routes also revealed significant differences between them as regards effectiveness for remediation. In addition, column studies using a {gamma}-camera to obtain real time images of a {sup 99m}Tc(VII) radiotracer were performed to visualize directly the relative performances of the magnetite sorbents against ultra-trace concentrations of metal oxyanion contaminants. Again, the magnetite produced from schwertmannite proved capable of retaining more ({approx}20%) {sup 99m}Tc(VII) than the magnetite produced from ferrihydrite, confirming that biomagnetite production for efficient environmental remediation can be fine-tuned through careful selection of the initial Fe(III) mineral

  4. POINT 2011: ENDF/B-VII.1 Beta2 Temperature Dependent Cross Section Library

    Energy Technology Data Exchange (ETDEWEB)

    Cullen, D E

    2011-04-07

    This report is one in the series of 'POINT' reports that over the years have presented temperature dependent cross sections for the then current version of ENDF/B. In each case I have used my personal computer at home and publicly available data and codes. I have used these in combination to produce the temperature dependent cross sections used in applications and presented in this report. I should mention that today anyone with a personal computer can produce these results. The latest ENDF/B-VII.1 beta2 data library was recently and is now freely available through the National Nuclear Data Center (NNDC), Brookhaven National Laboratory. This release completely supersedes all preceding releases of ENDF/B. As distributed the ENDF/B-VII.1 data includes cross sections represented in the form of a combination of resonance parameters and/or tabulated energy dependent cross sections, nominally at 0 Kelvin temperature. For use in our applications the ENDF/B-VII.1 library has been processed into cross sections at eight neutron reactor like temperatures, between 0 and 2100 Kelvin, in steps of 300 Kelvin (the exception being 293.6 Kelvin, for exact room temperature at 20 Celsius). It has also been processed to five astrophysics like temperatures, 1, 10, 100 eV, 1 and 10 keV. For reference purposes, 300 Kelvin is approximately 1/40 eV, so that 1 eV is approximately 12,000 Kelvin. At each temperature the cross sections are tabulated and linearly interpolable in energy. All results are in the computer independent ENDF-6 character format [R2], which allows the data to be easily transported between computers. In its processed form the POINT 2011 library is approximately 16 gigabyte in size and is distributed on one compressed DVDs (see, below for the details of the contents of each DVD).

  5. ENDF/B-VII.1 library - current and future trends in reaction data

    Science.gov (United States)

    Herman, Michal

    2011-10-01

    The ENDF/B-VII.0 library, made available in December 2006, has been the first major release of the US nuclear reaction data library in 16 years. Intensive validation proved generally good performance of the library but a number of deficiencies were discerned. In 2008 CSEWG decided to undertake multi-laboratory effort leading to VII.1 version, which is scheduled for release in December 2011. Highlights of the new library include (i) a consistent set of covariance data, for over 180 materials, targeting explicit needs of the Advanced Fuel Cycle Initiative, (ii) new R-matrix based evaluations for several light nuclei, (iii) evaluations for reactions on structural materials in both the fast neutron region and the resonance region, (iv) improvements of resonances regions and thermal cross sections for certain fission products and neutron absorber materials (Cd, Gd), (v) improvements in minor actinide evaluations for isotopes of U, Np, Pu, and Am, (vi) adoption of JENDL4.0 evaluations for the Cm, Bk, Cf, Es, Fm, and some other minor actinides, (vii) fission product yield advances for fission-spectrum neutrons and 14 MeV neutrons incident on 239Pu, and (viii) a new Decay Data sub-library. No significant changes are expected for the major actinides 235,8U and 239Pu, except reverting delayed neutron data to ENDF/B-VI.8 and adding covariances. Current update concerns only the most important neutron, fission yields, and decay data sub-libraries leaving the remaining 11 sub-libraries unchanged. Open issues and trends for the future releases of the ENDF/B will be discussed.

  6. PENGEMBANGAN LKS BERBASIS PROJECT BASED LEARNING UNTUK PEMBELAJARAN MATERI SEGITIGA DI KELAS VII

    Directory of Open Access Journals (Sweden)

    Dian Novita

    2016-06-01

    Full Text Available Penelitian ini merupakan penelitian pengembangan yang bertujuan untuk (1mengetahui karakteristik LKS berbasis Project Based Learning (PjBL yang valid dan praktis dalam pembelajaran materi segitiga di kelas VII; (2mengetahui efek potensial penggunaan LKS berbasis PjBL terhadap hasil belajar siswa dalam pembelajaran materi segitiga di kelas VII. Metode penelitian pengembangan yang digunakan adalah model  ADDIE (Analysis, Design, Development, Implementation, and Evaluation. Teknik pengumpulan data yang digunakan adalah walkthrough, tes hasil belajar dan angket. Dari tes hasil belajar, diperoleh bahwa sebesar 82,5% siswa telah mencapai kategori tuntas dan 17,5% belum tuntas dengan KKM 75. Hasil angket respon siswa menunjukkan bahwa untuk pelaksanaan pembelajaran menggunakan LKS berbasis PjBL telah mencapai kriteria kepraktisan. Kemudian untuk LKS berbasis PjBL yang telah didesain, divalidasi oleh beberapa pakar, kemudian direvisi berdasarkan saran dari para pakar tersebut. LKS yang telah direvisi tersebut dianggap telah memenuhi kriteria kevalidan dan kepraktisan sehingga dapat digunakan dalam pembelajaran materi segitiga di kelas VII. Kesimpulan dari penelitian ini adalah (1 LKS bebasis PjBL yang dikembangkan valid, tergambar dari konten (sesuai dengan kurikulum untuk materi segitiga, konstruk (sesuai dengan prinsip PjBL, dan bahasa (sesuai dengan ejaan yang disempurnakan (EYD. Kepraktisan tergambar dari proses kerja siswa dalam melaksanakan LKS dan hasil angket respon siswa terhadap pembelajaran menggunakan LKS berbasis PjBL; (2 dari hasil latihan yang dikerjakan oleh siswa, LKS berbasis PjBL memiliki efek potensial terhadap hasil belajar, yakni sebanyak 33 siswa termasuk kategori tuntas (82,5% dan 7 orang belum tuntas (17,5%, dengan KKM 75. Kata Kunci : LKS, Project Based Learning DOI: http://dx.doi.org/10.22342/jpm.10.2.3626.1-12

  7. In vitro amylolysis of pulse and hylon VII starches explained in terms of their composition, morphology, granule architecture and interaction between hydrolysed starch chains.

    Science.gov (United States)

    Maaran, S; Hoover, R; Vamadevan, V; Waduge, R N; Liu, Q

    2016-02-01

    The objective of this study was to understand the factors underlying the susceptibility of pulse (lablab bean, navy bean, rice bean, tepary bean, velvet bean, and wrinkled pea) and hylon VII starches towards in vitro hydrolysis by the combined action of pancreatin and amyloglucosidase. The time taken to reach an equivalent level of hydrolysis (50%) varied significantly among the starches. Changes to molecular order, crystallinity, double helical content, radial orientation of starch chains (polarized light), enthalpy and apparent amylose content during the progress of hydrolysis showed that rate and extent of hydrolysis were influenced both by the structure of the native starches at different levels (molecular, mesoscopic, microscopic) of granule organization, and by the extent of retrogradation between hydrolysed starch chains.

  8. Eksplorasi dan Pengamatan Intensitas Serangan Hama Penting Tanaman Tebu di PTPN VII, Cinta Manis Sumatera Selatan

    Directory of Open Access Journals (Sweden)

    Dewi Meidalima

    2015-03-01

    Full Text Available Penelitian bertujunan mengamati serangga-serangga hama penting yang menyerang tanaman tebu di PTPN VII, Cinta Manis Sumatera Selatan, dilakukan dari bulan Februari sampai September 2012. Metode survei langsung ke pertanaman dengan mengikuti jadual early warning system (EWS PTPN VII. Hasil penelitian menemukan serangga hama penting tanaman tebu ialah penggerek batang bergaris (Chilo saccharipaghus, penggerek batang berkilat (Chilo auricilius, dan penggerek pucuk (Scirpophaga nivella. Gejala serangan penggerek batang dan pucuk tebu ditemukanpada umur 2 bulan, serangan tinggi pada umur 3-5 bulan, hal ini berkaitan dengan cuaca. Pada saat penelitian dilakukan serangan tertinggi terjadi pada bulan Mei sampai Juli, dengan suhu, curah hujan, jumlah hari hujan, dan kelembaban nisbi berturut-turut adalah 26,6OC, 245,5 mm, 17 hari dan 98%. Serangan pada tanaman muda, menyebabkan kematian. Pada tanaman yang sudah membentuk ruas, gejala penggerek batang jelas terlihat dari luar jika daun tebu “diklentek”. Gejala serangan penggerek pucuk terlihat pada helai daun yang berlubang.Intensitas serangan penggerek batang tertinggi pada umur tebu 3 bulan (6,69%, sedangkan intensitas serangan (2,97% dan populasi penggerek pucuk (44,60 larva tertinggi pada umur 3,5 bulan. The research aimed to observe the important pest insects attacking sugarcane in PTPN VII, Cinta Manis,South Sumatra. Direct Survey methodto the crop by following the schedule ofearly warning system (EWS of PTPN VII, conducted from February to September 2012. The results found that important insect pests attacking sugarcane werestriped stem borer (Chilo saccharipaghus, shiny stem borer (Chilo auricilius, and shoot borers (Scirpophaga nivella. Symptoms attack of shotand stemborer of sugarcane were found at 2 months of sugarcane age, high attack at the age of 3-5 months, it is highly related to weather.The highest attack occurred in May and July, with the temperature, precipitation, number of rainy

  9. Procopius about the supreme god of the Slavs (Bella VII 14, 23: Two critical remarks

    Directory of Open Access Journals (Sweden)

    Loma Aleksandar

    2004-01-01

    Full Text Available A passage from Procopius, Bella VII 14, 23 is reconsidered, in which the beliefs of the heathen Slavs are described. It is shown to be corrupted. The main emendation proposed here consists in separating the word dçmioyrgón from the preceding tçz ástrapçz and connecting it with the following ápántõn: consequently, the supreme god of the Slavs is said not to be the producer of lightning, but the creator of the universe. .

  10. Underground Test Area Subproject Phase I Data Analysis Task. Volume VII - Tritium Transport Model Documentation Package

    Energy Technology Data Exchange (ETDEWEB)

    None

    1996-12-01

    Volume VII of the documentation for the Phase I Data Analysis Task performed in support of the current Regional Flow Model, Transport Model, and Risk Assessment for the Nevada Test Site Underground Test Area Subproject contains the tritium transport model documentation. Because of the size and complexity of the model area, a considerable quantity of data was collected and analyzed in support of the modeling efforts. The data analysis task was consequently broken into eight subtasks, and descriptions of each subtask's activities are contained in one of the eight volumes that comprise the Phase I Data Analysis Documentation.

  11. Aspectos literarios de la obra de don Joan de Castellanos : Capítulo VII

    Directory of Open Access Journals (Sweden)

    Mario Germán Romero

    1966-02-01

    Full Text Available Capitulo VII. Los discursos de Colón Es sabido que los escritores antiguos; principalmente los historiógrafos, introducían supuestos discursos en sus obras. Salustio consigna cuatro en sus Historias. Tito Livio los elabora con perfección y los pone en boca de políticos y generales importantes; en los treinta y cinco libros que se conservan de este autor encontramos unos cuatrocientos discursos, breves o extensos, que como los de Salustio fueron recopilados y editados para fines retóricos.

  12. Henry VII and the governement of the Italian cities (1310-1313

    Directory of Open Access Journals (Sweden)

    Gian Maria Varanini (a cura di

    2014-04-01

    Full Text Available The political project pursued by the emperor Henry VII to achieve the reconciliation and the reaffirmation of imperial sovereignty during his Italian expedition (1310-1313, could not be carried out apart from the decisive role of a small but influential group of employees trained in a typically Italian political culture. They are on one hand the notaries who oversee the writing of imperial documents, and on the other hand the heterogeneous group of vicars to whom the emperor entrusted the government of the city. The contributions collected here deepen various aspects concerning this political group, and show the ephemeral successes and failures of the Emperor's politics.

  13. Vald jagab tunnustusena välja esimesed viis aumärki / Anu Bollverk

    Index Scriptorium Estoniae

    Bollverk, Anu

    2007-01-01

    Türi valla 2. aastapäeva pidulikul tähistamisel jagab vald välja esimesed viis aumärki, avaldades austust ja tunnustust kahele silmapaistvale naisele ja kolmele noorele, kes on teinud koduvalla nime tuntuks ka kaugemal. Üks autasustatutest on parlamendiliige Kaia Iva, kes saab valla aumärgi kui Lõuna-Järvamaa nelja omavalitsuse ühinemisprotsessi aktiivseim eestvedaja. Aumärgi pälvisid ka flötist Mari Meentalo ja laulja Mikk Dede

  14. Interacción genotipo-ambiente de 135 líneas endogámicas recombinantes de sorgo para producción de biocombustibles G*E interaction of 135 recombinant inbred lines of sorghum for biofuel production

    Directory of Open Access Journals (Sweden)

    Armando Rodríguez García

    2012-02-01

    Full Text Available El sorgo (Sorghum bicolor (L Moench, es uno de los alimentos básicos para la población más pobre del mundo, que es también la que padece una situación de mayor inseguridad alimentaria. Este cultivo se adapta a un entorno agroecológico cálido y seco, donde es difícil cultivar otros cereales alimenticios. La formación de genotipos sobresalientes requiere evaluar los materiales genéticos en diferentes ambientes y determinar su interacción genotipo-ambiente. El objetivo del trabajo fue detectar y analizar la interacción genotipo-ambiente de un grupo de líneas endogámicas recombinantes y seleccionar las de mayor potencial, para ser utilizadas en la producción de biocombustibles. Se evaluaron 135 líneas endogámicas recombinantes de sorgo en diferentes localidades durante los años 2006, 2007 y 2008. Los datos de siete variables se analizaron como corresponde a un experimento en diseño de bloques al azar, en cada uno de varios ambientes. La interacción genotipo-ambiente, se analizó mediante el modelo AMMI. Fue altamente significativa, calificando las líneas endogámicas recombinantes por su magnitud y seleccionando las de menor magnitud, se determinó el mejor ambiente para el desarrollo adecuado de las líneas endogámicas recombinantes y se seleccionaron 13 líneas con mayor contenido de azúcar medido en °Bx.Sorghum (Sorghum bicolor (L Moench is one of the main basic foods forthe poorest people in the world, suffering from a situation of increased food insecurity. This crop is adapted to a warmer and drier agro-ecological environment, where it's difficult to grow other cereals. The formation of outstanding genotypes requires to evaluating the genetic materials in different environments and determines their genotype-environment interaction. The objective was to detect and analyze the genotype-environmentofagroup of recombinant inbred lines and select the highest potential to be used in the production of biofuels. 135 recombinant

  15. Comportamiento clínico epidemiológico del absceso dentoalveolar agudo en pacientes pertenecientes al área VII de Cienfuegos

    Directory of Open Access Journals (Sweden)

    Ana Belkys Hernández Millán

    2015-02-01

    Full Text Available Fundamento: el absceso dentoalveolar agudo es una de las urgencias estomatológicas y una de las principales enfermedades que afectan a la población, sin embargo existen muy pocos estudios sobre el tema.Objetivo: describir comportamiento clínico epidemiológico del absceso dentoalveolar agudo en pacientes pertenecientes al área VII, de Cienfuegos. Métodos: investigación observacional, descriptiva realizada de enero a diciembre de 2013 en el Área VII, de Cienfuegos. El universo fue de 672 pacientes y la muestra de 374, escogida mediante muestreo probabilístico aleatorio simple. El registro primario de datos y recolección de la información se obtuvo mediante la historia clínica individual previo consentimiento informado a los pacientes. Las principales variables fueron: edad, sexo, irritantes pulpares.Resultados: el sexo más afectado fue el femenino con 55,35 % y el grupo de edad el de 19 a 34 con 33,69 %.El irritante pulpar predominante fue el microbiano 59,36 %. Dentro de los factores iatrogénicos, los restos de tejidos cariados se destacaron con un 32,35 %.Conclusiones: existe un número elevado de pacientes con absceso dentoalveolar agudo, por lo que, como urgencia estomatológica, debe ser una preocupación del estomatólogo conocer las características, comportamiento y factores que desarrollan la enfermedad, para propiciar un trabajo integral en cuanto a la promoción de salud, prevención, curación y rehabilitación de los pacientes afectados.

  16. Cloning, periplasmic expression, purification and structural characterization of human ribosomal protein L10; Clonagem, expressao, purificacao e caracterizacao estrutural da proteina ribossomal L10 humana recombinante

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Larissa Miranda

    2009-07-01

    The ribosomal protein L10 (RP L10) is a strong candidate to be included in the class of tumor suppressor proteins. This protein, also denominated as QM, is known to participate in the binding of ribosomal subunits 60S and 40S and the translation of mRNAs. It has a molecular weight that varies between 24 and 26 kDa and an isoelectric point of (pI) 10.5. The sequence of the protein QM is highly conserved in mammals, plants, invertebrates, insects and yeast which indicates its critical functions in a cell. As a tumor suppressor, RP L10 has been studied in strains of Wilm's tumor (WT-1) and tumor cells in the stomach, where was observed a decrease in the amount of its mRNA. More recently, the RP L10 was found in low amounts in the early stages of prostate adenoma and showed some mutation in ovarian cancer, what indicates its role as a suppressor protein in the development of these diseases. It has also been described that this protein interacts with c-Jun and c-Yes inhibiting growth factors and consequently, cell division. This work has an important role on the establishment of soluble expression of QM to give base information for further studies on expression that aim to evaluate the specific regions where it acts binding the 60S and 40S ribosomal subunits and translation, as well as its binding to proto-oncogenes. The cDNA for QM protein was amplified by PCR and cloned into periplasmic expression vector p3SN8. The QM protein was expressed in E. coli BL21 (DE3) in the region of cytoplasm and periplasm, the best condition was obtained from the expression of the recombinant plasmid QM p1813{sub Q}M at 25 degree C or 30 degree C, the soluble protein was obtained with small amounts of contaminants. The assays of secondary structure showed that the QM protein is predominantly alpha-helix, but when it loses the folding, this condition changes and the protein is replaced by {beta}- sheet feature. (author)

  17. PENGARUH KETERAMPILAN MENGELOLA KELAS DAN GAYA MENGAJAR GURU TERHADAP KEAKTIFAN BELAJAR SISWA KELAS VII MATA PELAJARAN IPS TERPADU DI SMP NEGERI SE-KECAMATAN WEDUNG KABUPATEN DEMAK

    Directory of Open Access Journals (Sweden)

    Rifanah

    2014-06-01

    Full Text Available Tujuan Penelitian ini adalah mengetahui pengaruh keterampilan mengelola kelas, dan gaya mengajar guru terhadap keaktifan belajar siswa kelas VII SMP Negeri se-Kecamatan Wedung. Dalam penelitian ini populasi yang digunakan adalah seluruh siswa kelas VII SMP Negeri se-Kecamatan Wedung yang berjumlah 296 siswa. Penelitian ini menggunakan sampel yang berjumlah 81 sampel. Teknik pengambilan sampel menggunakan proportional random sampling. Variabel dalam penelitian ini adalah variabel bebas yaitu keterampilan mengelola kelas (XI, gaya mengajar guru (X2, dan keaktifan belajar siswa (Y sebagai variabel terikat. Metode pengumpulan data: dokumentasi dan angket. Metode analisis yaitu analisi deskriptif persentase. Hasil penelitian diperoleh persamaan regresi Y = 1,873+ 0,260 X1 + 0,670 X2. Secara deskriptif penelitian menunjukkan bahwa keterampilan mengelola kelas, dan gaya mengajar guru adalah baik. Pengaruh keterampilan mengelola kelas terhadap keaktifan belajar siswa sebesar 84,5%, gaya mengajar guru terhadap keaktifan belajar siswa sebesar 92,8%, pengarunya positif dan signifikan. Sedangkan pengaruh keterampilan mengelola kelas dan gaya mengajar guru secara bersama berpengaruh terhadap keaktifan belajar siswa sebesar 89,7% dan sisanya sebesar 10,3% dipengaruhi oleh faktor lain yang tidak diteliti. The purpose of this study was to determine the effect of classroom management skills, and the teaching style of teachers to students of class VII activity of SMP Wedung throughout the District. In this study population used is the entire Junior High School seventh grade students throughout the District Wedung totaling 296 students. This study used a sample of 81 samples. Sampling technique using proportional random sampling. The variable in this study is the independent variable classroom management skills (XI, the teacher's teaching style (X2, and the activity of student learning (Y as the dependent variable. Methods of data collection: documentation and

  18. [Chemical tests with Marrubium species. Official data on Marubii herba in Pharmacopoeia Hungarica VII].

    Science.gov (United States)

    Telek, E; Tõth, L; Botz, L; Máthé, I

    1997-01-01

    About 40 species of the Marrubium genus (Lamiaceae) are known of which 2 species (M. vulgare L. and M. peregrinum L.) and one hybrid (M. x paniculatum Desr.) can be found as native plants in Hungary. The above-ground parts of M. vulgare L. are official in Hungarian Pharmacopoeia VII. Active substances in Marrubii herba are labdane-structured bitter materials. Although the presence of furanic labdane diterpenes in the plant is known, the pharmacopoeia gives only microscopic tests, qualitative tests (for other parts of the plant and foreign organic matter) for the bitter value of Marrubii herba. We have examined the main terpenoid substances isolated with column, gel and preparative layer chromatography. Structure elucidations were performed by means of UV, mass and NMR spectroscopy. We have compared the changes in terpenoid-type compounds (premarrubiin and marrubiin) in plants during the vegetation period; in different Marrubium species and in the different extractions of horehound by means of thin layer chromatography and densitometry. By reason of our results we propose qualitative and quantitative chemical tests for the paragraph of Marrubii herba in Pharmacopoeia Hungarica VII.

  19. The HI Chronicles of LITTLE THINGS BCDs: VII Zw 403’s External Gas Cloud

    Science.gov (United States)

    Ashley, Trisha L.; Simpson, Caroline E.; Elmegreen, Bruce; Johnson, Megan C.; Pokhrel, Nau Raj

    2017-01-01

    Blue compact dwarf (BCD) galaxies are characterized by their concentrated bursts of star formation. Yet, for many BCDs, it is unclear what has triggered this activity. VII Zw 403 is a well-known BCD that is relatively isolated from other galaxies. Using the high angular and velocity resolution Very Large Array (VLA) atomic hydrogen (HI) data from the LITTLE THINGS1 survey, we study the detailed kinematics and morphology of VII Zw 403’s HI gas. High sensitivity HI Green Bank Telescope (GBT) observations were also used to search the surrounding area for companion galaxies and extended HI emission, but they did not result in detections of either. The VLA data show a kinematically and morphologically disturbed HI disk. From the VLA HI data cubes, we have separated out most of the emission from what is likely an external gas cloud that is in the line of sight of the HI disk. This external gas cloud appears to be accreting onto the disk and could trigger a future burst of star formation. 1Local Irregulars That Trace Luminosity Extremes, The HI Nearby Galaxy Survey; https://science.nrao.edu/science/surveys/ littlethings

  20. The helical phase of chiral nematic liquid crystals as the Bianchi VII(0) group manifold

    CERN Document Server

    Gibbons, G W

    2011-01-01

    We show that the optical structure of the helical phase of a chiral nematic is naturally associated with the Bianchi VII(0) group manifold. The Joets-Ribotta metric governing propagation of the extraordinary rays is invariant under the simply transitive action of the universal cover of the three dimensional Euclidean group of two dimensions. Thus extraordinary light rays are geodesics of a left-invariant metric on this Bianchi type VII(0) group. We are able to solve by separation of variables both the wave equation and the Hamilton-Jacobi equation for this metric. The former reduces to Mathieu's equation and the later to the quadrantal pendulum equation. We further discuss Maxwell's equations for uniaxial optical materials where the configuration is invariant under a group action. The material is not assumed to be impedance matched, thus going beyond the usual scope of transformation optics. We show that for a chiral nematic in its helical phase Maxwell's equations reduce to a generalised Mathieu equation. Ou...

  1. PENGEMBANGAN PROGRAM MULTIMEDIA UNTUK MENINGKATKAN PENGUASAAN KOSAKATA BAHASA INGGRIS SISWA KELAS VII SMP DI BANJARBARU

    Directory of Open Access Journals (Sweden)

    Rahmad Hidayat

    2014-05-01

    Full Text Available Penelitian ini bertujuan menghasilkan program multimedia interaktif berbasis Flash untuk meningkatkan penguasaan kosakata siswa kelas VII SMP Kota Banjarbaru. Penelitian ini meng-ukur kualitas program ditinjau dari validasi ahli, penilaian pengguna dan hasil pretest-posttest. Pengembangan dilakukan melalui lima tahapan, yaitu: (1 analisis awal, (2 desain, (3 pengem-bangan program, (4 evaluasi, dan (5 pengemasan produk akhir. Subjek uji coba adalah siswa dan guru Bahasa Inggris kelas VII di SMP Negeri 11 Banjarbaru. Instrumen yang digunakan dalam pengumpulan data adalah lembar validasi, angket guru dan siswa, dan pretest-posttest. Hasil penelitian menunjukkan: (1 kualitas program dari segi materi dan media termasuk dalam kategori sangat baik dengan rerata skor 4,30 dan 4,44; (2 kualitas program dari penilaian guru dan siswa masuk dalam kategori baik dengan rerata skor 3,79 (guru dan 4,18 (siswa; dan (3 terdapat peningkatan antara rerata skor hasil pretest (61,33 dengan posttest (85,2. Dengan demikian program multimedia yang dikembangkan cocok dan layak untuk digunakan dalam pembelajaran Bahasa Inggris di kelas. Kata kunci: pengembangan program, multimedia, kosakata, Flash

  2. VizieR Online Data Catalog: Effective collision strengths of Si VII (Sossah+, 2014)

    Science.gov (United States)

    Sossah, A. M.; Tayal, S. S.

    2017-08-01

    The purpose of present work is to calculate more accurate data for Si VII by using highly accurate target descriptions and by including a sufficient number of target states in the close-coupling expansion. We also included fine-structure effects in the close-coupling expansions to account for the relativistic effects. We used the B-spline Breit-Pauli R-matrix (BSR) codes (Zatsarinny 2006CoPhC.174..273Z) in our scattering calculations. The present method utilizes the term-dependent non-orthogonal orbital sets for the description of the target wave functions and scattering functions. The collisional and radiative parameters have been calculated for all forbidden and allowed transitions between the lowest 92 LSJ levels of 2s22p4, 2s2p5, 2p6, 2s22p33s, 2s22p33p, 2s22p33d, and 2s2p43s configurations of Si VII. (3 data files).

  3. On the recent history of star formation in the BCD galaxy VII Zw403

    CERN Document Server

    Silich, S A; Cairos, L M; Muñoz-Tunón, C

    2002-01-01

    The recent history of star formation in the BCD galaxy VII Zw403, is analysed with accounts for the dynamics of the remnant generated either by an instantaneous burst or by a continuous star formation event. We have re-observed VII Zw403 with a better sensitivity corresponding to the threshold H$_{\\alpha}$ flux $8.15 \\times 10^{-17}$ erg cm$^{-2}$ s$^{-1}$. The total H$_{\\alpha}$ luminosity derived from our data is much larger than reported before, and presents a variety of ionized filaments and incomplete shells superimposed on the diffuse H$_{\\alpha}$ emission. This result has a profound impact on the predicted properties of the starburst blown superbubble. Numerical calculations based on the previous H$_{\\alpha}$ data, predict two different scenarios of star formation able to match simultaneously all observed parameters. These are an instantaneous burst of star formation with a total mass of $5 \\times 10^5$ M$_{\\odot}$ and a star-forming event with a constant SFR = $4 \\times 10^{-3}$ M$_{\\odot}$ yr$^{-1}$,...

  4. Long circulating enzyme replacement therapy rescues bone pathology in mucopolysaccharidosis VII murine model.

    Science.gov (United States)

    Rowan, Daniel J; Tomatsu, Shunji; Grubb, Jeffrey H; Haupt, Bisong; Montaño, Adriana M; Oikawa, Hirotaka; Sosa, Angela C; Chen, Anping; Sly, William S

    2012-09-01

    Mucopolysaccharidosis (MPS) type VII is a lysosomal storage disease caused by deficiency of the lysosomal enzyme β-glucuronidase (GUS), leading to accumulation of glycosaminoglycans (GAGs). Enzyme replacement therapy (ERT) effectively clears GAG storage in the viscera. Recent studies showed that a chemically modified form of GUS (PerT-GUS), which escaped clearance by mannose 6-phosphate and mannose receptors and showed prolonged circulation, reduced CNS storage more effectively than native GUS. Clearance of storage in bone has been limited due to the avascularity of the growth plate. To evaluate the effectiveness of long-circulating PerT-GUS in reducing the skeletal pathology, we treated MPS VII mice for 12 weeks beginning at 5 weeks of age with PerT-GUS or native GUS and used micro-CT, radiographs, and quantitative histopathological analysis for assessment of bones. Micro-CT findings showed PerT-GUS treated mice had a significantly lower BMD. Histopathological analysis also showed reduced storage material and a more organized growth plate in PerT-GUS treated mice compared with native GUS treated mice. Long term treatment with PerT-GUS from birth up to 57 weeks also significantly improved bone lesions demonstrated by micro-CT, radiographs and quantitative histopathological assay. In conclusion, long-circulating PerT-GUS provides a significant impact to rescue of bone lesions and CNS involvement.

  5. Usefulness of recombinant human TSH-aided radioiodine doses administered in patients with differentiated thyroid carcinoma Administración de dosis terapéuticas de radioyodo luego de TSH recombinante en pacientes con carcinoma diferenciado de tiroides

    Directory of Open Access Journals (Sweden)

    Fabián Pitoia

    2006-04-01

    Full Text Available The published studies confirming the safety and efficacy of rhTSH for diagnostic purposes have led to an increased interest in its use for preparation for radioiodine (RI dose administration in patients with recurrent or persistent differentiated thyroid carcinoma (DTC. In order to establish the efficacy of RI therapy after rhTSH, we have reviewed 39 rhTSH-aided radioiodine treatment in a series of 28 DTC patients. Patients were divided into two groups: GI (n=17, with previous thyroid bed uptake and undetectable thyroglobulin (Tg levels under levothyroxine treatment and GII (n=11, with proven metastatic local or distant disease. Median follow-up after the first rhTSH-aided radioiodine treatment was 32 ± 13 months (range 8 to 54 months. Sixteen patients (94% in GI were rendered disease free and one patient was shown to have persistent disease. In GII, the post therapy whole body scan showed pathological uptakes in all cases: in four patients in lungs, in four in mediastinum and in three in lateral neck. In two patients with mediastinum uptake, Tg levels were undetectable after rhTSH. In the follow-up, two patients with lateral neck uptake were rendered disease free, four patients died (three due to thyroid cancer and five out of the remaining patients have persistent disease. In conclusion, rhTSH aided therapy was helpful to eliminate normal thyroid bed remnants in 16/17 (94% patients (GI. rhTSH stimulated Tg was undetectable in two patients with mediastinal metastasis. We believe that rhTSH is a good alternative to levothyroxine withdrawal for the treatment of DTC with radioactive iodine, increasing the quality of life in these patients. Caution should be recommended in the follow-up of unselected DTC patients only with stimulated Tg levels.Los estudios publicados que confirman la seguridad y eficacia de la TSH recombinante (rhTSH llevaron a un incremento en el interés para su uso como adyuvante terapéutico en el CDT (ablación o tratamiento

  6. Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women Sorologia utilizando o antígeno recombinante ROP2 no diagnóstico da toxoplasmose em mulheres grávidas

    Directory of Open Access Journals (Sweden)

    Miriam de Souza Macre

    2009-10-01

    Full Text Available Toxoplasma gondii causes severe fetal disease during acute infection in pregnant women, thus demanding early diagnosis for effective treatment and fetus preservation. Fetal tests are inefficient and risky, and diagnosis is based on maternal IgM serology, which had weak screening ability due to increased sensitivity, with alternative IgG avidity tests. Here, we performed ELISA and avidity assays using a recombinant T. gondii antigen, rROP2, in samples from 160 pregnant women screened from a large public hospital who were referred due to positive IgM assays. IgG serology and avidity assays were compared using whole T. gondii extract or rROP2. ELISA IgG detection with rROP2 showed good agreement with assays performed with T. gondii extract, but rROP2 IgG avidity assays were unrelated to whole extract antigen IgG avidity, regardless of the chaotrope used. These data show that avidity maturation is specific to individual antigen prevalence and immune response during infection. ELISA rROP2 IgG assays may be an alternative serological test for the diagnosis of toxoplasmosis during pregnancy, although our data do not support their use in avidity assays.A toxoplasmose causa grave doença fetal durante a infecção aguda da gestante, assim demanda diagnóstico precoce para tratamento efetivo e preservação fetal. Os testes fetais são de baixa eficiência e o diagnóstico é baseado na sorologia para IgM materna, que tem fraca capacidade de triagem devido a sensibilidade crescente dos testes, sendo uma alternativa os testes de avidez de IgG. Apresentamos aqui um ELISA e ensaio de avidez de IgG usando antígeno recombinante de T. gondii, rROP2, em amostras de 162 gestantes triadas para um grande hospital público por uma sorologia positiva para IgM. O ELISA IgG e o ensaio de avidez de IgG foram comparados usando o antígeno rROP2 ou extrato total de T. gondii. O ELISA usando rROP2 mostrou excelente concordância com os ensaios usando extrato de T. gondii

  7. Efeitos da eritropoetina recombinante humana em recém-nascidos pré-termo com doenças infecciosas Effects of recombinant human erythropoietin in preterm newborns with infectious diseases

    Directory of Open Access Journals (Sweden)

    Iara Flávia de Vasconcelos P. Aguiar

    2007-02-01

    Full Text Available OBJETIVO: Analisar os efeitos da eritropoetina recombinante humana (rHuEpo em recém-nascidos pré-termo com doenças infecciosas graves. MÉTODOS: Foi realizado um estudo controlado, não randomizado, em 34 recém-nascidos com diagnóstico de patologias infecciosas graves, peso de nascimento igual ou inferior a 1500 g, idade gestacional inferior a 35 semanas e estabilidade clínica. Os recém-nascidos designados para o tratamento com rHuEpo receberam a eritropoetina ß na dose de 400 UI/kg, duas vezes por semana, por via subcutânea. A suplementação oral com ferro foi iniciada quando os níveis de ferritina sérica foram inferiores a 60 mcg/L. O estudo foi realizado durante seis semanas ou até a alta hospitalar do paciente. Foram avaliados a eritropoese, o número de transfusões, o número de neutrófilos, a contagem de plaquetas e os episódios de novas infecções durante o tratamento com o hormônio. RESULTADOS: Houve aumento significativo do número de reticulócitos no grupo tratado; entretanto, não houve impacto sobre o número ou volume de transfusões. Não foram observadas alterações no número de neutrófilos ou plaquetas. CONCLUSÃO: O uso de rHuEpo em RNPT com doenças infecciosas, na dose de 800 UI/Kg/semana, foi efetivo para induzir eritropoese, sem ocorrerem alterações significativas sobre o número de neutrófilos ou plaquetas. Essa estratégia, associada ao controle rigoroso do volume de sangue retirado para exames, poderá ser benéfica na prevenção da anemia em RNPT com infecção grave.OBJECTIVE: To study the effects of recombinant human erythropoietin (rHuEpo in preterm newborns (PTNs with serious infectious diseases. METHODS: A not randomized case-control study was carried out in 34 preterm newborns with diagnosis of serious infectious pathologies, gestational age up to 35 weeks, birth weight less than 1500 g and clinical stability. Newborns selected for treatment with rHuEpo received 400 U/kg erythropoietin

  8. Bovine immunoprotection against Rhipicephalus (Boophilus microplus with recombinant Bm86-Campo Grande antigen Imunoproteção de bovinos contra Rhipicephalus (Boophilus microplus com antígeno recombinante Bm86-Campo Grande

    Directory of Open Access Journals (Sweden)

    Rodrigo Casquero Cunha

    2012-09-01

    Full Text Available The southern cattle fever tick, Rhipicephalus (Boophilus microplus, is no doubt the most economically important ectoparasite of cattle globally. The inappropriate use of chemical acaricides has driven the evolution of resistance in populations of R. (B. microplus. Anti-tick vaccines represent a technology that can be combined with acaricides in integrated control programs to mitigate the impact of R. (B. microplus. The recombinant form of Bm86 antigen from the Campo Grande (rBm86-CG strain of R. (B. microplus was produced using the Pichiapastoris expression system to test its ability to immunoprotect cattle against tick infestation. Secretion of rBm86-CG by P. pastoris through the bioprocess reported here simplified purification of the antigen. A specific humoral immune response was detected by ELISA in vaccinated cattle. Immunoblot results revealed that polyclonal antibodies from vaccinated cattle recognized a protein in larval extracts with a molecular weight corresponding to Bm86. The rBm86-CG antigen showed 31% efficacy against the Campo Grande strain of R. (B. microplus infesting vaccinated cattle. The rBm86-CG is an antigen that could be used in a polyvalent vaccine as part of an integrated program for the control of R. (B. microplus in the region that includes Mato Grosso do Sul.O carrapato Rhipicephalus (Boophilus microplus é, sem dúvidas, o ectoparasito economicamente mais importante para o gado a nível mundial. A utilização inadequada de acaricidas tem impulsionado a evolução da resistência em populações de R. (B. microplus. Vacinas contra o carrapato representam uma tecnologia que pode ser combinada com acaricidas em programas de controle integrado para diminuir o impacto de R. (B. microplus. A forma recombinante da Bm86 da cepa Campo Grande (rBm86-CG de R. (B. microplus foi produzido utilizando o sistema de expressão em Pichia pastoris para testar sua capacidade de imunoproteção ao gado contra a infestação de

  9. Low sensitivity of type VII collagen enzyme-linked immunosorbent assay in epidermolysis bullosa acquisita : serration pattern analysis on skin biopsy is required for diagnosis

    NARCIS (Netherlands)

    Terra, J. B.; Jonkman, M. F.; Diercks, G. F. H.; Pas, H. H.

    BackgroundThe type VII collagen (coll VII) enzyme-linked immunosorbent assay (ELISA) has been reported to have high sensitivity (>93%) and specificity (>96%) for diagnosing epidermolysis bullosa acquisita (EBA) in patients who are seropositive on indirect immunofluorescence on salt-split skin (SSS).

  10. Heterogeneity in ess transcriptional organization and variable contribution of the Ess/Type VII protein secretion system to virulence across closely related Staphylocccus aureus strains

    NARCIS (Netherlands)

    Kneuper, Holger; Cao, Zhen Ping; Twomey, Kate B; Zoltner, Martin; Jäger, Franziska; Cargill, James S; Chalmers, James; van der Kooi - Pol, Magda; van Dijl, Jan Maarten; Ryan, Robert P; Hunter, William N; Palmer, Tracy

    2014-01-01

    The Type VII protein secretion system, found in Gram-positive bacteria, secretes small proteins, containing a conserved W-x-G amino acid sequence motif, to the growth medium. Staphylococcus aureus has a conserved Type VII secretion system, termed Ess, which is dispensable for laboratory growth but r

  11. Heterogeneity in ess transcriptional organization and variable contribution of the Ess/Type VII protein secretion system to virulence across closely related Staphylocccus aureus strains

    NARCIS (Netherlands)

    Kneuper, Holger; Cao, Zhen Ping; Twomey, Kate B; Zoltner, Martin; Jäger, Franziska; Cargill, James S; Chalmers, James; van der Kooi - Pol, Magda; van Dijl, Jan Maarten; Ryan, Robert P; Hunter, William N; Palmer, Tracy

    The Type VII protein secretion system, found in Gram-positive bacteria, secretes small proteins, containing a conserved W-x-G amino acid sequence motif, to the growth medium. Staphylococcus aureus has a conserved Type VII secretion system, termed Ess, which is dispensable for laboratory growth but

  12. Low sensitivity of type VII collagen enzyme-linked immunosorbent assay in epidermolysis bullosa acquisita : serration pattern analysis on skin biopsy is required for diagnosis

    NARCIS (Netherlands)

    Terra, J. B.; Jonkman, M. F.; Diercks, G. F. H.; Pas, H. H.

    2013-01-01

    BackgroundThe type VII collagen (coll VII) enzyme-linked immunosorbent assay (ELISA) has been reported to have high sensitivity (>93%) and specificity (>96%) for diagnosing epidermolysis bullosa acquisita (EBA) in patients who are seropositive on indirect immunofluorescence on salt-split skin (SSS).

  13. Industrial Sector Technology Use Model (ISTUM): industrial energy use in the United States, 1974-2000. Volume 1: primary model documentation, Book 2, Chapters IV, V, VI, and VII

    Energy Technology Data Exchange (ETDEWEB)

    Bohn, R.E.; Herod, J.S.

    1978-06-19

    The Industrial Sector Technology Use Model provides a single framework for integrating all of the information and analysis into a set of predictions of industrial energy use. Given this volume of information which must be considered, this requires a set of computerized engineering-economic models. The design of these models is the subject of Chapter IV. The environmental factors in ISTUM are developed and presented in Chapter V. Chapter VI discusses the work accomplished by DATA Resources, Inc. in support of the ISTUM. A glossary, briefly defining special terms used in the ISTUM documentation, is given in Chapter VII. (MCW)

  14. The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145.

    Science.gov (United States)

    Ruzzi, M; Marconi, A; Saliola, M; Fabiani, L; Montebove, F; Frontali, L

    1997-03-30

    We report the sequence of a 8,061 bp fragment of Saccharomyces cerevisiae chromosome VII. Five open reading frames (ORFs) of at least 100 amino acids were identified. Three show similarities to the amino-acid sequence of known gene products. ORF G9374 corresponds to the gene coding for the yTAFII145 protein: a TBP-associated factor whose amino-acid sequence was previously reported (Reese et al., 1994). The remaining ORF does not display similarities to known sequences.

  15. PENGARUH MOTIVASI BELAJAR DAN KOMPETENSI PROFESIONAL GURU TERHADAP PRESTASI BELAJAR MATA PELAJARAN IPS EKONOMI SISWA KELAS VII SMP NEGERI 3 MAGELANG TAHUN PELAJARAN 2011/2012

    Directory of Open Access Journals (Sweden)

    Yunik Sulistyowati,

    2012-10-01

    Full Text Available Prestasi belajar merupakan indikator yang penting untuk mengukur keberhasilan proses belajar mengajar. Prestasi belajar dipengaruhi oleh banyak faktor, di antaranya motivasi belajar dan kompetensi profesional guru. Permasalahan dalam penelitian ini adalah adakah pengaruh motivasi belajar dan kompetensi profesional guru terhadap prestasi belajar IPS ekonomi siswa kelas VII SMP Negeri 3 Magelang Tahun Ajaran 2011/2012 baik secara simultan maupun parsial.Populasi penelitian ini adalah siswa kelas VII SMP Negeri 3 Magelang dengan sampel berjumlah 133 siswa. Variabel penelitian terdiri dari variabel bebas yaitu motivasi belajar (X1 dan kompetensi profesional guru (X2, dan variabel terikat yaitu prestasi belajar (Y. Metode pengumpulan data menggunakan angket dan dokumentasi. Hasil penelitian deskriptif persentase menunjukkan bahwa motivasi belajar siswa dalam kategori tinggi, kompetensi profesional guru� dalam kategori baik, dan prestasi belajar siswa dalam kategori tuntas. Hasil penelitian diperoleh persamaan regresi yaitu ?= 48,387 + 0,607(X1 + 0,101(X2. Dari hasil uji F diperoleh nilai signifikan sebesar 0,000 dan menunjukkan bahwa secara simultan ada pengaruh yang signifikan antara motivasi belajar dan kompetensi profesional guru terhadap prestasi belajar IPS ekonomi sebesar 47,7% sedangkan sisanya 52,3% dipengaruhi oleh faktor lain yang tidak dikaji dalam penelitian ini. Secara parsial menunjukkan bahwa ada pengaruh antara motivasi belajar terhadap prestasi belajar sebesar 22,09%, dan ada pengaruh kompetensi profesional guru terhadap prestasi belajar sebesar 28,40%. Learning achievement is an indicator that it is important to measure the success of the process of teaching and learning. Learning achievement are influenced by many factors, such as learning motivation and teacher�s professional competence.The problem in this study is whether there is influence of learning motivation and teacher�s professional competence toward learning

  16. Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV.

    Science.gov (United States)

    Roohani, Kiarash; Tan, Sheau Wei; Yeap, Swee Keong; Ideris, Aini; Bejo, Mohd Hair; Omar, Abdul Rahman

    2015-01-01

    A Newcastle disease virus (NDV) isolate designated IBS002 was isolated from a commercial broiler farm in Malaysia. The virus was characterised as a virulent strain based on the multiple basic amino acid motif of the fusion (F) cleavage site (112)RRRKGF(117) and length of the C-terminus extension of the hemagglutinin-neuraminidase (HN) gene. Furthermore, IBS002 was classified as a velogenic NDV with mean death time (MDT) of 51.2 h and intracerebral pathogenicity index (ICPI) of 1.76. A genetic distance analysis based on the full-length F and HN genes showed that both velogenic viruses used in this study, genotype VII NDV isolate IBS002 and genotype VIII NDV isolate AF2240-I, had high genetic variations with genotype II LaSota vaccine. In this study, the protection efficacy of the recombinant genotype VII NDV inactivated vaccine was also evaluated when added to an existing commercial vaccination program against challenge with velogenic NDV IBS002 and NDV AF2240-I in commercial broilers. The results indicated that both LaSota and recombinant genotype VII vaccines offered full protection against challenge with AF2240-I. However, the LaSota vaccine only conferred partial protection against IBS002. In addition, significantly reduced viral shedding was observed in the recombinant genotype VII-vaccinated chickens compared to LaSota-vaccinated chickens.

  17. 75 FR 36099 - Legislative Changes to Primary Care Loan Program Authorized Under Title VII of the Public Health...

    Science.gov (United States)

    2010-06-24

    ... Program Authorized Under Title VII of the Public Health Service Act AGENCY: Health Resources and Services... allopathic or osteopathic medicine. Below are details on how the ACA changes Section 723 of the Public Health... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH...

  18. GluVII:06--a highly conserved and selective anchor point for non-peptide ligands in chemokine receptors

    DEFF Research Database (Denmark)

    Rosenkilde, Mette M; Schwartz, Thue W

    2006-01-01

    to be crucially important for the binding and action of a number of non-peptide ligands in for example the CCR1, CCR2 and CCR5 receptors. It is proposed that in chemokine receptors in general GluVII:06 serves as a selective anchor point for the centrally located, positively charged nitrogen of the small molecule...

  19. 34 CFR 364.2 - What is the purpose of the programs authorized by chapter 1 of title VII?

    Science.gov (United States)

    2010-07-01

    ... SILS and CIL programs authorized by chapter 1 of title VII of the Act is to promote a philosophy of independent living (IL), including a philosophy of consumer control, peer support, self-help, self... integration and full inclusion of individuals with significant disabilities into the mainstream of...

  20. Title VII Enhancement Project for Compartiendo Culturas/Sharing Cultures 1995-96. Research Report on Educational Programs.

    Science.gov (United States)

    Houston Independent School District, TX. Dept. of Research and Evaluation.

    Compartiendo Culturas/Sharing Cultures, a Title VII Two-Way Developmental Bilingual Education Program at the Gary L. Herod Elementary School in the Houston Independent School District (Texas) was designed to end the isolation typically experienced by language minority students in traditional bilingual education and to provide language majority…

  1. 75 FR 51429 - Definitions Contained in Title VII of Dodd-Frank Wall Street Reform and Consumer Protection Act

    Science.gov (United States)

    2010-08-20

    ... floor; (III) A rate cap; (IV) A rate collar; (V) A cross-currency rate swap; (VI) A basis swap; (VII) A currency swap; (VIII) A foreign exchange swap; (IX) A total return swap; (X) An equity index swap; (XI) An... agreements, contracts, or transactions in foreign currency pursuant to section 2(c)(2).'' Security-Based......

  2. Marine Mammals, Coastal and River Issues. Alaska Sea Week Curriculum Series VII. Alaska Sea Grant Report 84-8.

    Science.gov (United States)

    Mickelson, Belle

    This curriculum guide is the last (Series VII) in a six-volume set that comprises the Sea Week Curriculum Series developed in Alaska. The guide lends itself to the sixth-grade curriculum but can be adapted to preschool, secondary, and adult education. Eight units contain 43 activities with worksheets that cover the following topics: (1) the values…

  3. Title VII Enhancement Project for Compartiendo Culturas/Sharing Cultures 1995-96. Research Report on Educational Programs.

    Science.gov (United States)

    Houston Independent School District, TX. Dept. of Research and Evaluation.

    Compartiendo Culturas/Sharing Cultures, a Title VII Two-Way Developmental Bilingual Education Program at the Gary L. Herod Elementary School in the Houston Independent School District (Texas) was designed to end the isolation typically experienced by language minority students in traditional bilingual education and to provide language majority…

  4. Free energy models for ice VII and liquid water derived from pressure, entropy, and heat capacity relations

    Science.gov (United States)

    Myint, Philip C.; Benedict, Lorin X.; Belof, Jonathan L.

    2017-08-01

    We present equations of state relevant to conditions encountered in ramp and multiple-shock compression experiments of water. These experiments compress water from ambient conditions to pressures as high as about 14 GPa and temperatures of up to several hundreds of Kelvin. Water may freeze into ice VII during this process. Although there are several studies on the thermodynamic properties of ice VII, an accurate and analytic free energy model from which all other properties may be derived in a thermodynamically consistent manner has not been previously determined. We have developed such a free energy model for ice VII that is calibrated with pressure-volume-temperature measurements and melt curve data. Furthermore, we show that liquid water in the pressure and temperature range of interest is well-represented by a simple Mie-Grüneisen equation of state. Our liquid water and ice VII equations of state are validated by comparing to sound speed and Hugoniot data. Although they are targeted towards ramp and multiple-shock compression experiments, we demonstrate that our equations of state also behave reasonably well at pressures and temperatures that lie somewhat beyond those found in the experiments.

  5. ENDF/B-VII.1 Nuclear Data for Science and Technology: Cross Sections, Covariances, Fission Product Yields and Decay Data

    Energy Technology Data Exchange (ETDEWEB)

    G. Palmiotti

    2011-12-01

    The ENDF/B-VII.1 library is our latest recommended evaluated nuclear data file for use in nuclear science and technology applications, and incorporates advances made in the five years since the release of ENDF/B-VII.0. These advances focus on neutron cross sections, covariances, fission product yields and decay data, and represent work by the US Cross Section Evaluation Working Group (CSEWG) in nuclear data evaluation that utilizes developments in nuclear theory, modeling, simulation, and experiment. The principal advances in the new library are: (1) An increase in the breadth of neutron reaction cross section coverage, extending from 393 nuclides to 418 nuclides; (2) Covariance uncertainty data for 185 of the most important nuclides, as documented in companion papers in this edition; (3) R-matrix analyses of neutron reactions on light nuclei, including isotopes of He, Li, and Be; (4) Resonance parameter analyses at lower energies and statistical high energy reactions at higher energies for isotopes of F, Cl, K, Ti, V, Mn, Cr, Ni, Zr and W; (5) Modifications to thermal neutron reactions on fission products (isotopes of Mo, Tc, Rh, Ag, Cs, Nd, Sm, Eu) and neutron absorber materials (Cd, Gd); (6) Improved minor actinide evaluations for isotopes of U, Np, Pu, and Am (we are not making changes to the major actinides 235,238U and 239Pu at this point, except for delayed neutron data, and instead we intend to update them after a further period of research in experiment and theory), and our adoption of JENDL-4.0 evaluations for isotopes of Cm, Bk, Cf, Es, Fm, and some other minor actinides; (7) Fission energy release evaluations; (8) Fission product yield advances for fission-spectrum neutrons and 14 MeV neutrons incident on 239Pu; and (9) A new Decay Data sublibrary. Integral validation testing of the ENDF/B-VII.1 library is provided for a variety of quantities: For nuclear criticality, the VII.1 library maintains the generally-good performance seen for VII.0 for a wide

  6. VII Seminario Internacional de Investigación en Odontología

    Directory of Open Access Journals (Sweden)

    Carolina Torres Rodríguez

    2016-07-01

    Full Text Available El VII Seminario de investigación en odontología, organizado por el programa de Maestría en Odontología, es un espacio de encuentro de estudiantes, investigadores nacionales e internacionales y asistentes, con el fin de compartir conocimientos y entablar redes de colaboración. También es una estrategia de comunicación, difusión, apropiación y transferencia de conocimiento y de los resultados de la investigación de los diferentes grupos con la comunidad científica nacional y/o internacional y un método para dar a conocer, promover e impulsar la investigación a nivel nacional, todo esto con el fin de brindar soluciones a las diferentes problemáticas de la profesión y de la salud oral y general.

  7. The architecture of EssB, an integral membrane component of the type VII secretion system.

    Science.gov (United States)

    Zoltner, Martin; Norman, David G; Fyfe, Paul K; El Mkami, Hassane; Palmer, Tracy; Hunter, William N

    2013-04-02

    The membrane-bound EssB is an integral and essential component of the bacterial type VII secretion system that can contribute to pathogenicity. The architecture of Geobacillus thermodenitrificans EssB has been investigated by combining crystallographic and EPR spectroscopic methods. The protein forms a dimer that straddles the cytoplasmic membrane. A helical fold is observed for the C-terminal segment, which is positioned on the exterior of the membrane. This segment contributes most to dimer formation. The N-terminal segment displays a structure related to the pseudokinase fold and may contribute to function by recognizing substrates or secretion system partners. The remaining part of EssB may serve as an anchor point for the secretion apparatus, which is embedded in the cytoplasmic membrane with the C-terminal domain protruding out to interact with partner proteins or components of peptidoglycan.

  8. Fission Product Yields from Fission Spectrum n+ 239Pu for ENDF/B-VII.1

    Science.gov (United States)

    Chadwick, M. B.; Kawano, T.; Barr, D. W.; Mac Innes, M. R.; Kahler, A. C.; Graves, T.; Selby, H.; Burns, C. J.; Inkret, W. C.; Keksis, A. L.; Lestone, J. P.; Sierk, A. J.; Talou, P.

    2010-12-01

    We describe a new cumulated fission product yield (FPY) evaluation for fission spectrum neutrons on plutonium that updates the ENDF/B-VI evaluation by England and Rider, for the forthcoming ENDF/B-VII.1 database release. We focus on FPs that are needed for high accuracy burnup assessments; that is, for inferring the number of fissions in a neutron environment. Los Alamos conducted an experiment in the 1970s in the Bigten fast critical assembly to determine fission product yields as part of the Interlaboratory Reaction Rate (ILRR) collaboration, and this has defined the Laboratory's fission standard to this day. Our evaluation includes use of the LANL-ILRR measurements (not previously available to evaluators) as well as other Laboratory FPY measurements published in the literature, especially the high-accuracy mass spectrometry data from Maeck and others. Because the measurement database for some of the FPs is small — especially for 99Mo — we use a meta-analysis that incorporates insights from other accurately-measured benchmark FP data, using R-value ratio measurements. The meta-analysis supports the FP measurements from the LANL-ILRR experiment. Differences between our new evaluations and ENDF/B-VI are small for some FPs (less than 1-2%-relative for 95Zr, 140Ba, 144Ce), but are larger for 99Mo (4%-relative) and 147Nd (5%-relative, at 1.5 MeV) respectively. We present evidence for an incident neutron energy dependence to the 147Nd fission product yield that accounts for observed differences in the FPY at a few-hundred keV average energy in fast reactors versus measurements made at higher average neutron energies in Los Alamos' fast critical assemblies. Accounting for such FPY neutron energy dependencies is important if one wants to reach a goal of determining the number of fissions to accuracies of 1-2%. An evaluation of the energy-dependence of fission product yields is given for all A values based on systematical trends in the measured data, with a focus on

  9. MCDHF Calculations and Beam-Foil EUV Spectra of Boron-Like Sodium Ions (Na VII

    Directory of Open Access Journals (Sweden)

    Per Jönsson

    2015-06-01

    Full Text Available Atomic data, such as wavelengths and line identifications, are necessary for many applications, especially in plasma diagnostics and for interpreting the spectra of distant astrophysical objects. The number of valence shell electrons increases the complexity of the computational problem. We have selected a five-electron ion, Na6+ (with the boron-like spectrum Na VII, for looking into the interplay of measurement and calculation. We summarize the available experimental work, perform our own extensive relativistic configuration interaction (RCI computations based on multi-configuration Dirac–Hartree–Fock (MCDHF wave functions, and compare the results to what is known of the level structure. We then discuss problems with databases that have begun to combine observations and computations.

  10. Pengembangan Perangkat Pembelajaran Strategi IDEAL Problem Solving Berbantuan Puzzquare Materi Luas Daerah Segiempat Kelas VII

    Directory of Open Access Journals (Sweden)

    Eny Susiana

    2011-12-01

    Full Text Available This research is classified as the developmental research. It develops learning materials using IDEAL problem solving strategy supported by Puzzquare for quadrilateral area for grade VII. The goals of this research are the development of learning materials using IDEAL problem solving strategy supported by Puzzquare is valid and learning process using that materials is effective. The development of learning materials on this research used 4-D model (Thiagarajan, Semmel and Semmel: (1 Define, (2 Design, (3 Develop, and (4 Disseminate. The developed learning-materials are (1 syllabus (2 lesson plan, (3 handbook, (4 student activity sheet, and (5 problem solving ability test. Keyword: The Development of Learning Material, IDEAL Problem Solving, Puzzquare

  11. Una lista de autores literarios en Plinio el Viejo: Naturalis Historia VII 107-117

    Directory of Open Access Journals (Sweden)

    Susana González Marín

    2003-06-01

    Full Text Available In NH VII 107-117 the Elder Pliny offers a selection of Greek and Roman notabilities who achieved prominence through their ingenium. This selection follows the model set by περὶ τεχνιτῶν treatises, especially Varro's De Poetis. Pliny's selection is based on two considerations: the glory achieved by each person; the opinions expressed by an authority, in most cases a politician. According to Pliny, the four Roman authors selected (Ennius, Virgil, Varro, Cicero served as paragons of excellence for the Roman people. Through his selection, Pliny defines his own attitude about the literary tradition and the intellectual elite in Rome: his idea of literature as a public service, the practical value of his work, his preference for prose and the increasingly strong influence exerted by experts on Roman Society.

  12. Holographic fermions at strong translational symmetry breaking: a Bianchi-VII case study

    CERN Document Server

    Bagrov, A; Krikun, A; Schalm, K; Zaanen, J

    2016-01-01

    It is presently unknown how strong lattice potentials influence the fermion spectral function of the holographic strange metals predicted by the AdS/CFT correspondence. This embodies a crucial test for the application of holography to condensed matter experiments. We show that for one particular momentum direction this spectrum can be computed for arbitrary strength of the effective translational symmetry breaking potential of the so-called Bianchi-VII geometry employing ordinary differential equations. Deep in the strange metal regime we find rather small changes to the single-fermion response computed by the emergent quantum critical IR, even when the potential becomes relevant in the infra-red. However, in the regime where holographic quasi-particles occur, defining a Fermi surface in the continuum, they acquire a finite lifetime at any finite potential strength. At the transition from irrelevancy to relevancy of the Bianchi potential in the deep infra-red the quasi-particle remnants disappear completely a...

  13. Testing of ENDF71x: A new ACE-formatted neutron data library based on ENDF/B-VII.1

    Energy Technology Data Exchange (ETDEWEB)

    Gardiner, S. J.; Conlin, J. L.; Kiedrowski, B. C.; Lee, M. B.; Parsons, D. K.; White, M. C. [Los Alamos National Laboratory, Los Alamos, NM 87544 (United States)

    2013-07-01

    The ENDF71x library [1] is the most thoroughly tested set of ACE-format data tables ever released by the Nuclear Data Team at Los Alamos National Laboratory (LANL). It is based on ENDF/B-VII. 1, the most recently released set of evaluated nuclear data files produced by the US Cross Section Evaluation Working Group (CSEWG). A variety of techniques were used to test and verify the ENDF7 1x library before its public release. These include the use of automated checking codes written by members of the Nuclear Data Team, visual inspections of key neutron data, MCNP6 calculations designed to test data for every included combination of isotope and temperature as comprehensively as possible, and direct comparisons between ENDF71x and previous ACE library releases. Visual inspection of some of the most important neutron data revealed energy balance problems and unphysical discontinuities in the cross sections for some nuclides. Doppler broadening of the total cross sections with increasing temperature was found to be qualitatively correct. Test calculations performed using MCNP prompted two modifications to the MCNP6 source code and also exposed bad secondary neutron yields for {sup 231,233}Pa that are present in both ENDF/B-VII.1 and ENDF/B-VII.0. A comparison of ENDF71x with its predecessor ACE library, ENDF70, showed that dramatic changes have been made in the neutron cross section data for a number of isotopes between ENDF/B-VII.0 and ENDF/B-VII.1. Based on the results of these verification tests and the validation tests performed by Kahler, et al. [2], the ENDF71x library is recommended for use in all Monte Carlo applications. (authors)

  14. Keefektifan Strategi Pembelajaran React Pada Kemampuan Siswa Kelas VII Aspek Komunikasi Matematis

    Directory of Open Access Journals (Sweden)

    A.T. Arifin

    2014-06-01

    Full Text Available AbstrakTujuan penelitian ini adalah mengetahui pembelajaran dengan strategi REACT efektif ter-hadap kemampuan komunikasi matematis siswa. Metode pengumpulan data dilakukan de-ngan metode dokumentasi, tes, dan observasi. Hasil uji proporsi menunjukkan bahwa hasil belajar siswa kelas eksperimen pada aspek kemampuan komunikasi matematis telah men-capai ketuntasan klasikal, mencapai lebih dari 80 % yaitu sebesar 96,7%. Dilihat dari nilai rata-rata tes kemampuan komunikasi matematis kelas eksperimen  adalah 83,61 sedangkan kelas kontrol adalah 73,79 dapat disimpulkan bahwa kemampuan komunikasi matematis siswa kelas eksperimen lebih baik daripada kemampuan komunikasi matematis siswa kon-trol. Hasil penelitian menunjukkan bahwa penerapan strategi pembelajaran REACT efektif terhadap kemampuan komunikasi matematis siswa materi segiempat kelas VII SMP Negeri 1 Gembong. Kata kunci:      keefektifan, kemampuan komunikasi matematis, Relating Experiencing Applying Cooperating Transferring (REACT  AbstractThe purpose of this study was to determine the effectiveness of the application of REACT learning strategy approach to mathematic communication ability of students. Methods of data collection is done by the method of documentation, testing, and observation. The test results showed that the proportion of student learning outcomes in the experimental class with the aspects of mathematic communication ability has reached the classical completeness, reached more than 80% is equal to 96.7%. Judging from the value of the average test learners' ability to mathematic communication experimental class was 83.61 while the control class is 73.79 it can be concluded that the mathematic communication skills of learners experimental classes are better than mathematic communication abilities of learners control class. The results showed that the application of REACT learning strategy approach effective to mathematic communication abilities of students of class VII

  15. Aspects of haemostatic function in healthy subjects with microalbuminuria--a potential atherosclerotic risk factor

    DEFF Research Database (Denmark)

    Jensen, J S; Myrup, B; Borch-Johnsen, K

    1995-01-01

    .6 micrograms/min): Coagulation factors: blood platelet count and mean volume, plasma Factor VII antigen concentration and coagulant activity, and plasma concentrations of prothrombin fragment 1 + 2, thrombin-antithrombin III complexes, fibrinogen, and fibrinopeptide A; fibrinolytic and endothelial factors...

  16. Type VII Collagen is Enriched in the Enamel Organic Matrix Associated with the Dentin-Enamel Junction of Mature Human Teeth

    Science.gov (United States)

    McGuire, Jacob D.; Walker, Mary P.; Mousa, Ahmad; Wang, Yong; Gorski, Jeff P.

    2014-01-01

    The inner enamel region of erupted teeth is known to exhibit higher fracture toughness and crack growth resistance than bulk phase enamel. However, an explanation for this behavior has been hampered by the lack of compositional information for the residual enamel organic matrix. Since enamel-forming ameloblasts are known to express type VII collagen and type VII collagen null mice display abnormal amelogenesis, the aim of this study was to determine whether type VII collagen is a component of the enamel organic matrix at the dentin-enamel junction (DEJ) of mature human teeth. Immunofluorescent confocal microscopy of demineralized tooth sections localized type VII collagen to the organic matrix surrounding individual enamel rods near the DEJ. Morphologically, immunoreactive type VII collagen helical-bundles resembled the gnarled-pattern of enamel rods detected by Coomassie Blue staining. Western blotting of whole crown or enamel matrix extracts also identified characteristic Mr=280 and 230 kDa type VII dimeric forms, which resolved into 75 and 25 kDa bands upon reduction. As expected, the collagenous domain of type VII collagen was resistant to pepsin digestion, but was susceptible to purified bacterial collagenase. These results demonstrate the inner enamel organic matrix in mature teeth contains macromolecular type VII collagen. Based on its physical association with the DEJ and its well-appreciated capacity to complex with other collagens, we hypothesize that enamel embedded type VII collagen fibrils may contribute not only to the structural resilience of enamel, but may also play a role in bonding enamel to dentin. PMID:24594343

  17. Diseño y construcción de vectores de transferencia para la obtención de virus vaccinia Ankara modificado (MVA recombinantes Design and construction of transfer vectors in order to obtain recombinant modified vaccinia virus Ankara (MVA

    Directory of Open Access Journals (Sweden)

    M. F. Ferrer

    2007-09-01

    Full Text Available El virus vaccinia Ankara modificado (MVA constituye un buen candidato para el desarrollo de vectores virales de expresión no replicativos porque no replica en la mayoría de las células de mamíferos. Para la producción de MVA recombinantes es fundamental disponer de vectores de transferencia que, por recombinación homóloga con el genoma viral, permitan introducir los genes de interés en regiones no esenciales para la replicación in vitro. En este trabajo se diseñaron y obtuvieron los vectores de transferencia denominados VT-MHA y VT-MTK que portan las regiones correspondientes a las posiciones 1-303 y 608-948 del gen MVA165R y 1-244 y 325-534 del gen MVA086R, respectivamente, las que flanquean un sitio de clonado múltiple para la inserción de los genes foráneos. En dichos vectores se clonaron los casetes para la expresión de los genes lac Z o uid A, y la actividad de las enzimas marcadoras b-galactosidasa y b-glucuronidasa se confirmó in situ. Además, utilizando el vector denominado VT-MTK-GUS, se obtuvieron y aislaron MVA recombinantes puros que portan y expresan el gen uid A. Los resultados obtenidos constituyen las herramientas básicas para establecer la metodología de obtención de MVA recombinantes, con el propósito de desarrollar localmente vectores virales no replicativos candidatos a vacunas.Modified Vaccinia virus Ankara (MVA constitutes a good candidate for the development of non-replicative expression viral vectors because it does not replicate in most of mammalian cells. It is essential, for the production of recombinant MVA, the availability of transfer vectors which allow the introduction of desired genes into non-essential regions for in vitro viral replication, by homologous recombination with the viral genome. In the present work, the transfer vectors named VT-MHA and VT-MTK were designed and obtained. They carried genomic regions corresponding to 1- 303 and 608-948 positions of the MVA165R gene and 1-244 and

  18. ENDF/B-VII.1 Neutron Cross Section Data Testing with Critical Assembly Benchmarks and Reactor Experiments

    Science.gov (United States)

    Kahler, A. C.; MacFarlane, R. E.; Mosteller, R. D.; Kiedrowski, B. C.; Frankle, S. C.; Chadwick, M. B.; McKnight, R. D.; Lell, R. M.; Palmiotti, G.; Hiruta, H.; Herman, M.; Arcilla, R.; Mughabghab, S. F.; Sublet, J. C.; Trkov, A.; Trumbull, T. H.; Dunn, M.

    2011-12-01

    The ENDF/B-VII.1 library is the latest revision to the United States' Evaluated Nuclear Data File (ENDF). The ENDF library is currently in its seventh generation, with ENDF/B-VII.0 being released in 2006. This revision expands upon that library, including the addition of new evaluated files (was 393 neutron files previously, now 423 including replacement of elemental vanadium and zinc evaluations with isotopic evaluations) and extension or updating of many existing neutron data files. Complete details are provided in the companion paper [M. B. Chadwick et al., "ENDF/B-VII.1 Nuclear Data for Science and Technology: Cross Sections, Covariances, Fission Product Yields and Decay Data," Nuclear Data Sheets, 112, 2887 (2011)]. This paper focuses on how accurately application libraries may be expected to perform in criticality calculations with these data. Continuous energy cross section libraries, suitable for use with the MCNP Monte Carlo transport code, have been generated and applied to a suite of nearly one thousand critical benchmark assemblies defined in the International Criticality Safety Benchmark Evaluation Project's International Handbook of Evaluated Criticality Safety Benchmark Experiments. This suite covers uranium and plutonium fuel systems in a variety of forms such as metallic, oxide or solution, and under a variety of spectral conditions, including unmoderated (i.e., bare), metal reflected and water or other light element reflected. Assembly eigenvalues that were accurately predicted with ENDF/B-VII.0 cross sections such as unmoderated and uranium reflected 235U and 239Pu assemblies, HEU solution systems and LEU oxide lattice systems that mimic commercial PWR configurations continue to be accurately calculated with ENDF/B-VII.1 cross sections, and deficiencies in predicted eigenvalues for assemblies containing selected materials, including titanium, manganese, cadmium and tungsten are greatly reduced. Improvements are also confirmed for selected

  19. ENDF/B-VII.1 Neutron Cross Section Data Testing with Critical Assembly Benchmarks and Reactor Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Kahler, A.C.; Herman, M.; Kahler,A.C.; MacFarlane,R.E.; Mosteller,R.D.; Kiedrowski,B.C.; Frankle,S.C.; Chadwick,M.B.; McKnight,R.D.; Lell,R.M.; Palmiotti,G.; Hiruta,H.; Herman,M.; Arcilla,R.; Mughabghab,S.F.; Sublet,J.C.; Trkov,A.; Trumbull,T.H.; Dunn,M.

    2011-12-01

    The ENDF/B-VII.1 library is the latest revision to the United States Evaluated Nuclear Data File (ENDF). The ENDF library is currently in its seventh generation, with ENDF/B-VII.0 being released in 2006. This revision expands upon that library, including the addition of new evaluated files (was 393 neutron files previously, now 423 including replacement of elemental vanadium and zinc evaluations with isotopic evaluations) and extension or updating of many existing neutron data files. Complete details are provided in the companion paper [M. B. Chadwick et al., 'ENDF/B-VII.1 Nuclear Data for Science and Technology: Cross Sections, Covariances, Fission Product Yields and Decay Data,' Nuclear Data Sheets, 112, 2887 (2011)]. This paper focuses on how accurately application libraries may be expected to perform in criticality calculations with these data. Continuous energy cross section libraries, suitable for use with the MCNP Monte Carlo transport code, have been generated and applied to a suite of nearly one thousand critical benchmark assemblies defined in the International Criticality Safety Benchmark Evaluation Project's International Handbook of Evaluated Criticality Safety Benchmark Experiments. This suite covers uranium and plutonium fuel systems in a variety of forms such as metallic, oxide or solution, and under a variety of spectral conditions, including unmoderated (i.e., bare), metal reflected and water or other light element reflected. Assembly eigenvalues that were accurately predicted with ENDF/B-VII.0 cross sections such as unmoderated and uranium reflected {sup 235}U and {sup 239}Pu assemblies, HEU solution systems and LEU oxide lattice systems that mimic commercial PWR configurations continue to be accurately calculated with ENDF/B-VII.1 cross sections, and deficiencies in predicted eigenvalues for assemblies containing selected materials, including titanium, manganese, cadmium and tungsten are greatly reduced. Improvements are also

  20. Polimorfismos FVII IVS7, FVII R353Q en el gen del Factor VII y FXIII Val34Leu y su asociación con el riesgo de infarto agudo del miocardio en pacientes costarricenses FVII polymorphisms IVS7, FVII R353Q in FVII gene and FXIII Val34Leu and its association with the risk of acute myocardial infartion in Costa Rica Patients

    Directory of Open Access Journals (Sweden)

    Lizbeth Salazar-Sánchez

    2012-12-01

    Full Text Available Resumen Los Niveles aumentados de los factores de la coagulación como el FVII y FXIII se han asociado con infarto agudo del Miocardio (IAM. Los estudios de biología molecular han permitido detectar varias mutaciones en los genes del FVII y FXIII que se han asociado al riesgo de enfermedad coronaria. Métodos: Se estudiaron 186 pacientes que sufrieron infarto agudo del miocardio y 201 controles sanos. Se determinaron los polimorfismos FXIII (Val34Leu; FVII, IV(S7, FVII (R353Q, según las técnicas descritas. Cabe destacar que esta investigación siguió los lineamientos de bioética. Resultados: La edad de los pacientes fue de 46,2 años (147 hombres/39 mujeres, y la de los controles fue de 46 años (141 hombres/ 60 mujeres. La prevalencia de las mutaciones obtenidas tanto en pacientes como controles fue: FVII IVS7 OR: 0,60 (0,26-1,38 p=0,193 y FVIIR353Q OR: 0,81(0,61-1,08 p=0,729; respectivamente. El fenotipo Leu/Leu tiene más elevada prevalencia en los casos controles que en los pacientes infartados, al hacer el ajuste de factores de riesgo cardiovascular, se demostró que este fenotipo es un factor protector para el desarrollo del IAM OR: 0,66(0,47-0,93 p=0,01. Los factores de riesgo tradicionales fueron estadísticamente significativos. En el FVII, y en el FVII IVS se encontraron nuevas variantes (4 y 8, no descritas previamente. Conclusión: El FXIII Val34Leu se presenta como factor protector contra el IAM, y ninguno de los polimorfismos del FVII se encontró asociados como factor de riesgo para IAM. El FXIII Val34Leu ha sido descrito como un facilitador de la activación del factor XIII, durante la fase final de la coagulación, incrementando y acelerando la estabilización de la fibrina, confiriendo más resistencia ante la fibrinólisis. Se incrementan el interés de este polimorfismo en el IAM y en especial para los pacientes que fueran sometidos a terapia antifibrinolítica.Increased levels of coagulation factors such as FVII and

  1. ANALYSIS OF Treponema pallidum RECOMBINANT ANTIGENS FOR DIAGNOSIS OF SYPHILIS BY WESTERN BLOTTING TECHNIQUE Análise de antígenos recombinantes de Treponema pallidum no diagnóstico da sífilis utilizando a técnica de Western Blotting

    Directory of Open Access Journals (Sweden)

    Neuza Satomi SATO

    1999-03-01

    Full Text Available Three GST fusion recombinant antigen of Treponema pallidum, described as GST-rTp47, GST-rTp17 and GST-rTp15 were analyzed by Western blotting techniques. We have tested 53 serum samples: 25 from patients at different clinical stages of syphilis, all of them presenting anti-treponemal antibody, 25 from healthy blood donors and three from patients with sexually transmitted disease (STD other than syphilis. Almost all samples from patients with syphilis presented a strong reactivity with GST-rTp17 antigen. Some samples were non-reactive or showed a weak reaction with GST-rTp47 and/or GST-rTp15, and apparently there was no correlation with the stage of disease. There was no seropositivity among blood donors. No sample reacted with purified GST. We concluded that due to their specificity these recombinant antigens can be used as GST fusion protein for development of syphilis diagnostic assays.Os antígenos recombinantes de Treponema pallidum GST-rTp47, GST-rTp17 e GST-rTp15, produzidos em fusão com glutationa S-transferase (GST em E. coli, foram analisados quanto ao potencial diagnóstico da sífilis pela técnica de Western blotting. Foram testadas 53 amostras, sendo 25 de pacientes em diferentes estágios clínicos da sífilis, com resultados positivos no teste treponêmico clássico; 25 amostras procedentes de doadores de banco de sangue, com sorologia negativa e 3 de pacientes com doença sexualmente transmissível não relacionado à sífilis. Todas as amostras de pacientes com sífilis apresentaram alta reatividade com o antígeno GST-rTp17. Quanto aos antígenos GST-rTp47 e GST-Tp15 verificou-se uma variação na presença ou na intensidade da reação em diferentes amostras de pacientes com sífilis, sem mostrar correlação com o estágio da doença. Nenhuma reatividade contra quaisquer desses antígenos foi observada com as amostras do grupo controle. Nenhuma das amostras testadas apresentaram reatividade com a GST purificada. A

  2. Reporte de dos casos de linfadenitis supurada causada por bacilo de Calmette-Guerin tratados con interferón gamma recombinante Report of two cases of suppurated lymphadenitis caused by Bacillus Calmette-Guerin treated with recombinant γ-interferon

    Directory of Open Access Journals (Sweden)

    Gladys Abreu Suárez

    2008-09-01

    Full Text Available La linfadenitis supurada es una complicación poco frecuente que sigue a la vacunación con bacilo de Calmette-Guerin. Se describen los casos de dos niños con reacciones adversas graves inducidas por esta vacuna, en ambos casos, linfadenitis regional supurada y abscedada, un mes después de nacidos. Después de cursos infructuosos de cirugía y quimioterapia, ambos recibieron interferón gamma recombinante por vía intramuscular, en una dosis inicial de 50 000 UI/kg (máximo: 1 000 000 UI, diariamente durante las primeras 4 semanas, y se disminuyó luego la frecuencia de administración. Esta citoquina fue bien tolerada, solo se presentaron complicaciones con fiebre, que fueron controladas bien con antipiréticos. El interferón gamma recombinante puede constituir una nueva y efectiva alternativa terapéutica para el tratamiento de la linfadenitis supurada causada por este bacilo.Suppurative lymphadenitis is a non frequent complication following Bacillus Calmette-Guerin (BCG vaccination. Two paediatric patients with adverse reactions induced by the BCG vaccine are presented, both with suppurative and abscessed regional lymphadenitis, one month after birth. After failed courses of surgery and chemotherapy, they were treated with 50 000 IU/Kg (maximum: 1 000 000 IU of recombinant interferon (IFN gamma, intramuscularly, daily during 4 weeks and 3 or 2 tpw afterwards. The first case, a nursing girl with family history of tuberculosis, had a rapid involution of the lesions since the first month of treatment, with drainage ceasing and gradual disappearance of the inflammatory signs. At the end of the 6 months of treatment, residues of the lesions were imperceptible and new adenopathies or relapses were not detected during 4 years of follow up. The second case, a boy without family history of tuberculosis, presented more lesions. The signs of marked improvement were observed in the whole affected region one year after IFN gamma started. Their

  3. Activity of recombinant factor VIIa under different conditions in vitro

    DEFF Research Database (Denmark)

    Bladbjerg, Else-Marie; Jespersen, Jørgen

    2008-01-01

    , but no effects on clotting time indicating that haemodilution does not affect clot formation, but the clot formed at high haemodilution may not be so firm. In conclusion, the activity of recombinant activated factor VII was affected in vitro by pH, temperature, and haemodilution. Additional studies are necessary...... investigated the in-vitro effects of pH, temperature, and haemodilution on the activity of recombinant activated factor VII. Samples from eight healthy volunteers were spiked with recombinant activated factor VII (final concentration 1.7 microg/ml) and adjusted to pH 6.0, 6.5, 7.0, and 7.4 or analysed at 30...... activity in plasma. Significant effects of pH were observed for clotting time, clot formation time, maximum clot firmness, and factor VII coagulant activity in the direction of longer clot formation times and less firm clots with decreasing pH. Temperature had significant effects on clotting time, clot...

  4. Publication and dating of Parts IV-VII of Brauer & Bergenstamm's Die Zweiflügler des Kaiserlichen Museums zu Wien (1889-1894).

    Science.gov (United States)

    Evenhuis, Neal L

    2014-04-22

    Based on new evidence, the dates of publication of the journal and separate versions of parts IV-VII of Brauer & Bergenstamm's Die Zweiflügler des Kaiserlichen Museums zu Wien are given, correcting those dates previously published.

  5. Isolation of a membrane protein complex for type VII secretion in Staphylococcus aureus.

    Science.gov (United States)

    Aly, Khaled A; Anderson, Mark; Ohr, Ryan Jay; Missiakas, Dominique

    2017-09-05

    The ESAT6-like secretion system (ESS) of Staphylococcus aureus promotes effector protein transport across the bacterial envelope. Genes in the ESS cluster are required for S. aureus establishment of persistent abscess lesions and the modulation of immune responses during blood stream infections. The biochemical functions of most of the ESS gene products, specifically the identity of secretion machine components, are however unknown. Earlier work demonstrated that deletion of essB, which encodes a membrane protein, abolishes S. aureus ESS secretion. Loss of function mutations truncating the essB gene product cause dominant-negative phenotypes on ESS secretion, suggesting that EssB may be a central component of the secretion machinery. To test this prediction, we purified native and affinity-tagged EssB from staphylococcal membranes via dodecyl-maltoside extraction, identifying a complex assembled from five proteins, EsaA, EssA, EssB, EssD and EsxA. All five proteins are essential for secretion, as knock-out mutations in the corresponding genes abolish ESS transport. Biochemical and bacterial two-hybrid analyses revealed a direct interaction between EssB and EsaA that, by engaging a mobile machine component, EsxA, may also recruit EssA and EssD.IMPORTANCE Type VII secretion systems support the lifestyle of Gram-positive bacteria, including important human pathogens such as Bacillus anthracis, Mycobacterium tuberculosis, and Staphylococcus aureus Genes encoding SpoIIIE-FtsK-like ATPases and WXG100 secreted products are conserved features of type VII secretion pathways, however most of the genes in T7SS clusters are not conserved between different bacterial species. Here we isolate a complex of proteins from the membranes of S. aureus that appears to represent the core secretion machinery, designated ESS (ESAT-6-like secretion system). These results suggest that three membrane proteins, EsaA, EssB and EssA form a secretion complex that associates with EssC, the Spo

  6. ENDF/B-VII.0: Next Generation Evaluated Nuclear Data Library for Nuclear Science and Technology

    Energy Technology Data Exchange (ETDEWEB)

    Chadwick, M B; Oblozinsky, P; Herman, M; Greene, N M; McKnight, R D; Smith, D L; Young, P G; MacFarlane, R E; Hale, G M; Haight, R C; Frankle, S; Kahler, A C; Kawano, T; Little, R C; Madland, D G; Moller, P; Mosteller, R; Page, P; Talou, P; Trellue, H; White, M; Wilson, W B; Arcilla, R; Dunford, C L; Mughabghab, S F; Pritychenko, B; Rochman, D; Sonzogni, A A; Lubitz, C; Trumbull, T H; Weinman, J; Brown, D; Cullen, D E; Heinrichs, D; McNabb, D; Derrien, H; Dunn, M; Larson, N M; Leal, L C; Carlson, A D; Block, R C; Briggs, B; Cheng, E; Huria, H; Kozier, K; Courcelle, A; Pronyaev, V; der Marck, S

    2006-10-02

    We describe the next generation general purpose Evaluated Nuclear Data File, ENDF/B-VII.0, of recommended nuclear data for advanced nuclear science and technology applications. The library, released by the U.S. Cross Section Evaluation Working Group (CSEWG) in December 2006, contains data primarily for reactions with incident neutrons, protons, and photons on almost 400 isotopes. The new evaluations are based on both experimental data and nuclear reaction theory predictions. The principal advances over the previous ENDF/B-VI library are the following: (1) New cross sections for U, Pu, Th, Np and Am actinide isotopes, with improved performance in integral validation criticality and neutron transmission benchmark tests; (2) More precise standard cross sections for neutron reactions on H, {sup 6}Li, {sup 10}B, Au and for {sup 235,238}U fission, developed by a collaboration with the IAEA and the OECD/NEA Working Party on Evaluation Cooperation (WPEC); (3) Improved thermal neutron scattering; (4) An extensive set of neutron cross sections on fission products developed through a WPEC collaboration; (5) A large suite of photonuclear reactions; (6) Extension of many neutron- and proton-induced reactions up to an energy of 150 MeV; (7) Many new light nucleus neutron and proton reactions; (8) Post-fission beta-delayed photon decay spectra; (9) New radioactive decay data; and (10) New methods developed to provide uncertainties and covariances, together with covariance evaluations for some sample cases. The paper provides an overview of this library, consisting of 14 sublibraries in the same, ENDF-6 format, as the earlier ENDF/B-VI library. We describe each of the 14 sublibraries, focusing on neutron reactions. Extensive validation, using radiation transport codes to simulate measured critical assemblies, show major improvements: (a) The long-standing underprediction of low enriched U thermal assemblies is removed; (b) The {sup 238}U, {sup 208}Pb, and {sup 9}Be reflector

  7. ENDF/B-VII.0: Next Generation Evaluated Nuclear Data Library for Nuclear Science and Technology

    Science.gov (United States)

    Chadwick, M. B.; Obložinský, P.; Herman, M.; Greene, N. M.; McKnight, R. D.; Smith, D. L.; Young, P. G.; MacFarlane, R. E.; Hale, G. M.; Frankle, S. C.; Kahler, A. C.; Kawano, T.; Little, R. C.; Madland, D. G.; Moller, P.; Mosteller, R. D.; Page, P. R.; Talou, P.; Trellue, H.; White, M. C.; Wilson, W. B.; Arcilla, R.; Dunford, C. L.; Mughabghab, S. F.; Pritychenko, B.; Rochman, D.; Sonzogni, A. A.; Lubitz, C. R.; Trumbull, T. H.; Weinman, J. P.; Brown, D. A.; Cullen, D. E.; Heinrichs, D. P.; McNabb, D. P.; Derrien, H.; Dunn, M. E.; Larson, N. M.; Leal, L. C.; Carlson, A. D.; Block, R. C.; Briggs, J. B.; Cheng, E. T.; Huria, H. C.; Zerkle, M. L.; Kozier, K. S.; Courcelle, A.; Pronyaev, V.; van der Marck, S. C.

    2006-12-01

    We describe the next generation general purpose Evaluated Nuclear Data File, ENDF/B-VII.0, of recommended nuclear data for advanced nuclear science and technology applications. The library, released by the U.S. Cross Section Evaluation Working Group (CSEWG) in December 2006, contains data primarily for reactions with incident neutrons, protons, and photons on almost 400 isotopes, based on experimental data and theory predictions. The principal advances over the previous ENDF/B-VI library are the following: (1) New cross sections for U, Pu, Th, Np and Am actinide isotopes, with improved performance in integral validation criticality and neutron transmission benchmark tests; (2) More precise standard cross sections for neutron reactions on H, 6Li, 10B, Au and for 235,238U fission, developed by a collaboration with the IAEA and the OECD/NEA Working Party on Evaluation Cooperation (WPEC); (3) Improved thermal neutron scattering; (4) An extensive set of neutron cross sections on fission products developed through a WPEC collaboration; (5) A large suite of photonuclear reactions; (6) Extension of many neutron- and proton-induced evaluations up to 150 MeV; (7) Many new light nucleus neutron and proton reactions; (8) Post-fission beta-delayed photon decay spectra; (9) New radioactive decay data; (10) New methods for uncertainties and covariances, together with covariance evaluations for some sample cases; and (11) New actinide fission energy deposition. The paper provides an overview of this library, consisting of 14 sublibraries in the same ENDF-6 format as the earlier ENDF/B-VI library. We describe each of the 14 sublibraries, focusing on neutron reactions. Extensive validation, using radiation transport codes to simulate measured critical assemblies, show major improvements: (a) The long-standing underprediction of low enriched uranium thermal assemblies is removed; (b) The 238U and 208Pb reflector biases in fast systems are largely removed; (c) ENDF/B-VI.8 good

  8. PENGARUH KEMANDIRIAN BELAJAR DAN PENGGUNAAN UANG SAKU TERHADAP PRESTASI BELAJAR KOMPETENSI DASAR EKONOMI KELAS VII DAN VIII

    Directory of Open Access Journals (Sweden)

    Apri Dwi Astuti

    2012-10-01

    Full Text Available Kemandirian belajar membantu siswa untuk bertanggung jawab dan menumbuhkan kesadaran belajar. Penggunaan uang saku menumbuhkan jiwa mandiri, jujur dan komitmen. Adapun hasil observasi awal menunjukkan masih ada siswa kels VII dan VIII SMP Semesta untuk kompetensi dasar ekonomi dibawah KKM. Tujuan penelitian ini untuk mengetahui pengaruh kemandirian belajar dn penggunaan uang saku terhadap prestasi belajar kompetensi dasar ekonomi kelas VII dan VIII SMP Semesta. Populasi penelitian ini adalah semua siswa kelas VII dan VIII SMP Semesta Boarding School Semarang. Variabel penelitian ini adalah kemandirian belajar dan penggunaan uang saku sebagai variabel bebas, sedangkan untuk� variabel terikat adalah prestasi belajar kompetensi dasar ekonomi. Data diambil dengan metode wawancara, kuesioner dan dokumentasi guru. Analisis data menggunakan analisis regresi linear berganda.Berdasarkan penelitian secara simultan menunjukkan adanya pengaruh antara kemandirian belajar dan penggunaan uang saku terhadap prestasi belajar kompetensi dasar ekonomi sebesar 51,3%. Secara parsial, pengaruh kemandirian belajar terhadap prestasi belajar kompetensi dasar ekonomi sebesar 66,2% dan pengaruh penggunaan uang saku terhadap prestasi belajar kompetensi dasar ekonomi sebesar 62,5%.Berdasarkan hasil penelitian diatas disimpulkan bahwa kemandirian belajar dan penggunaan uang saku berpengaruh positif baik secara simultan maupun parsial terhadap prestasi belajar kompetensi dasar ekonomi kelas VII dan VIII SMP Semesta Boarding school semarang tahun pelajaran 2011/2012. Saran yang berkaitan dengan penelitian ini yaitu : 1 Sekolah disarankan untuk memberikan batasan siswa pulang ke rumah agar dapat membantu siswa beradaptasi dengan cepat dan mudah tinggal di asrama. 2 Orang tua disarankan untuk memberikan uang saku yang disesuaikan dengan kebutuhan siswa. 3 Siswa disarankan untuk meningkatkan kemandirian dalam aspek belajar terutama bagi mata pelajaran yang masih kurang dari

  9. ENDF/B-VII.1 Nuclear Data for Science and Technology: Cross Sections, Covariances, Fission Product Yields and Decay Data

    Energy Technology Data Exchange (ETDEWEB)

    Chadwick, M.B.; Herman, M.; Author(s): Chadwick,M.B.; Herman,M.; Oblozinsky,P.; Dunn,M.E.; Danon,Y.; Kahler,A.C.; Smith,D.L.; Pritychenko,B.; Arbanas,G.; Arcilla,R.; Brewer,R.; Brown,D.A.; Capote,R.; Carlson,A.D.; Cho,Y.S.; Derrien,H.; Guber,K.; Hale,G.M.; Hoblit,S.; Holloway,S.: Johnson,T.D.; Kawano,T.; Kiedrowski,B.C.; Kim,H.; Kunieda,S.; Larson,N.M.; Leal,L.; Lestone,J.P.; Little,R.C.; McCutchan,E.A.; MacFarlane,R.E.; MacInnes,M.; Mattoon,C.M.; McKnight,R.D.; Mughabghab,S.F.; Nobre,G.P.A.; Palmiotti,G.; Palumbo,A.; Pigni,M.T.; Pronyaev,V.G.; Sayer,R.O.; Sonzogni,A.A.; Summers,N.C.; Talou,P.; Thompson,I.J.; Trkov,A.; Vogt,R.L.; van der Marck,S.C.; Wallner,A.; White,M.C.; Wiarda,D.; Young,P.G.

    2011-12-01

    The ENDF/B-VII.1 library is our latest recommended evaluated nuclear data file for use in nuclear science and technology applications, and incorporates advances made in the five years since the release of ENDF/B-VII.0. These advances focus on neutron cross sections, covariances, fission product yields and decay data, and represent work by the US Cross Section Evaluation Working Group (CSEWG) in nuclear data evaluation that utilizes developments in nuclear theory, modeling, simulation, and experiment. The principal advances in the new library are: (1) An increase in the breadth of neutron reaction cross section coverage, extending from 393 nuclides to 423 nuclides; (2) Covariance uncertainty data for 190 of the most important nuclides, as documented in companion papers in this edition; (3) R-matrix analyses of neutron reactions on light nuclei, including isotopes of He, Li, and Be; (4) Resonance parameter analyses at lower energies and statistical high energy reactions for isotopes of Cl, K, Ti, V, Mn, Cr, Ni, Zr and W; (5) Modifications to thermal neutron reactions on fission products (isotopes of Mo, Tc, Rh, Ag, Cs, Nd, Sm, Eu) and neutron absorber materials (Cd, Gd); (6) Improved minor actinide evaluations for isotopes of U, Np, Pu, and Am (we are not making changes to the major actinides {sup 235,238}U and {sup 239}Pu at this point, except for delayed neutron data and covariances, and instead we intend to update them after a further period of research in experiment and theory), and our adoption of JENDL-4.0 evaluations for isotopes of Cm, Bk, Cf, Es, Fm, and some other minor actinides; (7) Fission energy release evaluations; (8) Fission product yield advances for fission-spectrum neutrons and 14 MeV neutrons incident on {sup 239}Pu; and (9) A new decay data sublibrary. Integral validation testing of the ENDF/B-VII.1 library is provided for a variety of quantities: For nuclear criticality, the VII.1 library maintains the generally-good performance seen for VII.0

  10. ENDF/B-VII.1 Nuclear Data for Science and Technology: Cross Sections, Covariances, Fission Product Yields and Decay Data

    Science.gov (United States)

    Chadwick, M. B.; Herman, M.; Obložinský, P.; Dunn, M. E.; Danon, Y.; Kahler, A. C.; Smith, D. L.; Pritychenko, B.; Arbanas, G.; Arcilla, R.; Brewer, R.; Brown, D. A.; Capote, R.; Carlson, A. D.; Cho, Y. S.; Derrien, H.; Guber, K.; Hale, G. M.; Hoblit, S.; Holloway, S.; Johnson, T. D.; Kawano, T.; Kiedrowski, B. C.; Kim, H.; Kunieda, S.; Larson, N. M.; Leal, L.; Lestone, J. P.; Little, R. C.; McCutchan, E. A.; MacFarlane, R. E.; MacInnes, M.; Mattoon, C. M.; McKnight, R. D.; Mughabghab, S. F.; Nobre, G. P. A.; Palmiotti, G.; Palumbo, A.; Pigni, M. T.; Pronyaev, V. G.; Sayer, R. O.; Sonzogni, A. A.; Summers, N. C.; Talou, P.; Thompson, I. J.; Trkov, A.; Vogt, R. L.; van der Marck, S. C.; Wallner, A.; White, M. C.; Wiarda, D.; Young, P. G.

    2011-12-01

    The ENDF/B-VII.1 library is our latest recommended evaluated nuclear data file for use in nuclear science and technology applications, and incorporates advances made in the five years since the release of ENDF/B-VII.0. These advances focus on neutron cross sections, covariances, fission product yields and decay data, and represent work by the US Cross Section Evaluation Working Group (CSEWG) in nuclear data evaluation that utilizes developments in nuclear theory, modeling, simulation, and experiment. The principal advances in the new library are: (1) An increase in the breadth of neutron reaction cross section coverage, extending from 393 nuclides to 423 nuclides; (2) Covariance uncertainty data for 190 of the most important nuclides, as documented in companion papers in this edition; (3) R-matrix analyses of neutron reactions on light nuclei, including isotopes of He, Li, and Be; (4) Resonance parameter analyses at lower energies and statistical high energy reactions for isotopes of Cl, K, Ti, V, Mn, Cr, Ni, Zr and W; (5) Modifications to thermal neutron reactions on fission products (isotopes of Mo, Tc, Rh, Ag, Cs, Nd, Sm, Eu) and neutron absorber materials (Cd, Gd); (6) Improved minor actinide evaluations for isotopes of U, Np, Pu, and Am (we are not making changes to the major actinides 235,238U and 239Pu at this point, except for delayed neutron data and covariances, and instead we intend to update them after a further period of research in experiment and theory), and our adoption of JENDL-4.0 evaluations for isotopes of Cm, Bk, Cf, Es, Fm, and some other minor actinides; (7) Fission energy release evaluations; (8) Fission product yield advances for fission-spectrum neutrons and 14 MeV neutrons incident on 239Pu; and (9) A new decay data sublibrary. Integral validation testing of the ENDF/B-VII.1 library is provided for a variety of quantities: For nuclear criticality, the VII.1 library maintains the generally-good performance seen for VII.0 for a wide range

  11. Investigation of inconsistent ENDF/B-VII.1 independent and cumulative fission product yields with proposed revisions

    Energy Technology Data Exchange (ETDEWEB)

    Pigni, Marco T [ORNL; Francis, Matthew W [ORNL; Gauld, Ian C [ORNL

    2015-01-01

    A recent implementation of ENDF/B-VII. independent fission product yields and nuclear decay data identified inconsistencies in the data caused by the use of updated nuclear scheme in the decay sub-library that is not reflected in legacy fission product yield data. Recent changes in the decay data sub-library, particularly the delayed neutron branching fractions, result in calculated fission product concentrations that are incompatible with the cumulative fission yields in the library, and also with experimental measurements. A comprehensive set of independent fission product yields was generated for thermal and fission spectrum neutron induced fission for 235,238U and 239,241Pu in order to provide a preliminary assessment of the updated fission product yield data consistency. These updated independent fission product yields were utilized in the ORIGEN code to evaluate the calculated fission product inventories with experimentally measured inventories, with particular attention given to the noble gases. An important outcome of this work is the development of fission product yield covariance data necessary for fission product uncertainty quantification. The evaluation methodology combines a sequential Bayesian method to guarantee consistency between independent and cumulative yields along with the physical constraints on the independent yields. This work was motivated to improve the performance of the ENDF/B-VII.1 library in the case of stable and long-lived cumulative yields due to the inconsistency of ENDF/B-VII.1 fission p;roduct yield and decay data sub-libraries. The revised fission product yields and the new covariance data are proposed as a revision to the fission yield data currently in ENDF/B-VII.1.

  12. Thermal expansivity, bulk modulus, and melting curve of H2O-ice VII to 20 GPa

    Science.gov (United States)

    Fei, Yingwei; Mao, Ho-Kwang; Hemley, Russell J.

    1993-01-01

    Equation of state properties of ice VII and fluid H2O at high pressures and temperatures have been studied experimentally from 6 to 20 GPa and 300-700 K. The techniques involve direct measurements of the unit-cell volume of the solid using synchrotron X-ray diffraction with an externally heated diamond-anvil cell. The pressure dependencies of the volume and bulk modulus of ice VII at room temperature are in good agreement with previous synchrotron X-ray studies. The thermal expansivity was determined as a function of pressure and the results fit to a newly proposed phenomenological relation and to a Mie-Gruneisen equation of state formalism. The onset of melting of ice VII was determined directly by X-ray diffraction at a series of pressures and found to be in accord with previous volumetric determinations. Thermodynamic calculations based on the new data are performed to evaluate the range of validity of previously proposed equations of state for fluid water derived from static and shock-wave compression experiments and from simulations.

  13. Self-diffusion of protons in H2O ice VII at high pressures: Anomaly around 10 GPa.

    Science.gov (United States)

    Noguchi, Naoki; Okuchi, Takuo

    2016-06-21

    The self-diffusion of ice VII in the pressure range of 5.5-17 GPa and temperature range of 400-425 K was studied using micro Raman spectroscopy and a diamond anvil cell. The diffusion was monitored by observing the distribution of isotope tracers: D2O and H2 (18)O. The diffusion coefficient of hydrogen reached a maximum value around 10 GPa. It was two orders of magnitude greater at 10 GPa than at 6 GPa. Hydrogen diffusion was much faster than oxygen diffusion, which indicates that protonic diffusion is the dominant mechanism for the diffusion of hydrogen in ice VII. This mechanism is in remarkable contrast to the self-diffusion in ice Ih that is dominated by an interstitial mechanism for the whole water molecule. An anomaly around 10 GPa in ice VII indicates that the rate-determining process for the proton diffusion changes from the diffusion of ionic defects to the diffusion of rotational defects, which was suggested by proton conductivity measurements and molecular dynamics simulations.

  14. ORNL Resolved Resonance Covariance Generation for ENDF/B-VII.1

    Science.gov (United States)

    Leal, L.; Guber, K.; Wiarda, D.; Arbanas, G.; Derrien, H.; Sayer, R.; Larson, N.; Dunn, M.

    2012-12-01

    Resonance-parameter covariance matrix (RPCM) evaluations in the resolved resonance regionwere done at the Oak Ridge National Laboratory (ORNL) for the chromium isotopes, titanium isotopes, 19F, 58Ni, 60Ni, 35Cl, 37Cl, 39K, 41K, 55Mn, 233U, 235U, 238U, and 239Pu using the computer code SAMMY. The retroactive approach of the code SAMMY was used to generate the RPCMs for 233U. For 235U, the approach used for covariance generation was similar to the retroactive approach with the distinction that real experimental data were used as opposed to data generated from the resonance parameters. RPCMs for 238U and 239Pu were generated together with the resonance parameter evaluations. The RPCMs were then converted in the ENDF format using the FILE32 representation. Alternatively, for computer storage reasons, the FILE32 was converted in the FILE33 cross section covariance matrix (CSCM). Both representations were processed using the computer code PUFF-IV. This paper describes the procedures used to generate the RPCM and CSCM in the resonance region for ENDF/B-VII.1. The impact of data uncertainty in nuclear reactor benchmark calculations is also presented.

  15. Testing New Actinide Cross Sections Proposed for ENDF/B-VII

    Science.gov (United States)

    MacFarlane, Robert E.

    2005-05-01

    Our Nuclear Physics Group has worked over the last several years to improve the ENDF/B actinide cross sections, especially as measured against the famous series of fast-spectrum critical experiments performed at Los Alamos under such names as Godiva, Jezebel, the Flattops, and Bigten. The new evaluations include changes in the high-energy fission cross sections, nubar, elastic scattering, inelastic scattering, fission spectra, and delayed neutrons that combine to significantly improve the calculated results for the Los Alamos critical assemblies. As a happy byproduct of this work, we found that the new evaluations also removed about half of a long-standing discrepancy in calculations of thermal-reactor critical experiments using lattices of low-enriched uranium oxide rods. In the meantime, work at the Oak Ridge National Laboratory has resulted in new proposed resonance parameters for U238. When these low-energy data are combined with the new high-energy data from Los Alamos, they have the effect of removing most of the rest of the problem for thermal lattices. Although more work will be done at both Los Alamos and Oak Ridge, including making the evaluations consistent with the new standards, the results presented in this talk provide promise that ENDF/B-VII will result in good improvements for users at both high and low neutron energies.

  16. Outer Children Marriages Status After Constitutional Court Decision No: 46/PUU-VII/2010

    Directory of Open Access Journals (Sweden)

    Sri Budi Purwaningsih

    2014-01-01

    Full Text Available The decision of the Constitutional Court of the Republic of Indonesia No.46/PUU-VIII / 2010 dated 17 February 2012, granted the judicial review of Article 43 (1 of Law No. 1 of 1974 on Marriage by deciding that the article should read "Children who are born outside of marriage just had a civil relationship with her mother and her mother's family as well as with men as a father who can be proved based on science and technology and / or evidence, has blood ties according to law, including a civil relationship with his father's family". This Indonesian Constitutional Court's decision bring Juridical consequence that illegitimate children not only have a legal relationship with her mother, but also has a legal relationship with the father (biological and his father's family, as long as it is proven with science and technology. The Constitutional Court's decision is a starting point in the legal protection of illegitimate children, namely the "right alignment" between the illegitimate child with the legitimate son. Illegitimate children have the rights to demand their civil rights toward their father (biological as the same rights obtained by the legitimate son. How To Cite: Purwaningsih, S. (2016. Outer Children Marriages Status After Constitutional Court Decision No: 46/PUU-VII/2010. Rechtsidee, 1(1, 119-130. doi:http://dx.doi.org/10.21070/jihr.v1i1.99

  17. MT71x: Multi-Temperature Library Based on ENDF/B-VII.1

    Energy Technology Data Exchange (ETDEWEB)

    Conlin, Jeremy Lloyd [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Parsons, Donald Kent [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Gray, Mark Girard [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Lee, Mary Beth [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); White, Morgan Curtis [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-12-16

    The Nuclear Data Team has released a multitemperature transport library, MT71x, based upon ENDF/B-VII.1 with a few modifications as well as additional evaluations for a total of 427 isotope tables. The library was processed using NJOY2012.39 into 23 temperatures. MT71x consists of two sub-libraries; MT71xMG for multigroup energy representation data and MT71xCE for continuous energy representation data. These sub-libraries are suitable for deterministic transport and Monte Carlo transport applications, respectively. The SZAs used are the same for the two sub-libraries; that is, the same SZA can be used for both libraries. This makes comparisons between the two libraries and between deterministic and Monte Carlo codes straightforward. Both the multigroup energy and continuous energy libraries were verified and validated with our checking codes checkmg and checkace (multigroup and continuous energy, respectively) Then an expanded suite of tests was used for additional verification and, finally, verified using an extensive suite of critical benchmark models. We feel that this library is suitable for all calculations and is particularly useful for calculations sensitive to temperature effects.

  18. PENGEMBANGAN MEDIA PEMBELAJARAN KOSAKATA BAHASA INGGRIS BERBANTUAN KOMPUTER UNTUK SISWA SMP KELAS VII

    Directory of Open Access Journals (Sweden)

    Bastani Bastani

    2014-05-01

    Full Text Available Penelitian ini bertujuan: (1 menghasilkan media pembelajaran kosakata bahasa Inggris berbantuan komputer untuk siswa SMP kelas VII; dan (2 mengetahui kelayakan media pada aspek materi, pemrograman, dan tampilan. Penelitian ini adalah penelitian dan pengembangan dengan menggunakan model pengembangan Borg & Gall dan Alessi & Trollip’s yang telah dimodifikasi. Penelitian ini dilaksanakan melalui tiga tahap, yaitu perencanaan, desain, dan pengembangan. Tahap perencanaan meliputi penetapan tujuan dan penganalisisan kebutuhan. Tahap desain meliputi penyelenggaraan focus group discussion, pembuatan flowchart, storyboard, penyiapan materi, dan bahan pendukung. Tahap pengembangan meliputi pembuatan aplikasi berdasarkan flowchart dan storyboard, validasi ahli dan validasi pengguna. Instrumen penelitian yang digunakan adalah kuesioner/angket dan tes. Hasil penelitian dan pengembangan ini adalah: (1 produk media pembelajaran kosakata bahasa Inggris berbantuan komputer dalam bentuk CD pembelajaran dan print out produk; dan (2 kelayakan media ditinjau dari aspek materi termasuk kriteria sangat layak dengan rata-rata skor 4,47 pada skala lima, aspek pemrograman termasuk kriteria sangat layak dengan rata-rata skor 4,21 dan aspek tampilan termasuk kriteria sangat layak dengan rata-rata skor 4,44. Kata kunci: pengembangan, media pembelajaran, kosakata, pembelajaran berbantuan komputer.

  19. PWR ENDF/B-VII cross-section libraries for ORIGEN-ARP

    Energy Technology Data Exchange (ETDEWEB)

    McGraw, C. [Dept. of Nuclear Engineering, Texas A and M Univ., 3133 TAMU, College Station, TX 77843-3133 (United States); Ilas, G. [Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831-6172 (United States)

    2012-07-01

    New pressurized water reactor (PWR) cross-section libraries were generated for use with the ORIGEN-ARP depletion sequence in the SCALE nuclear analysis code system. These libraries are based on ENDF/B-VII nuclear data and were generated using the two-dimensional depletion sequence, TRITON/NEWT, in SCALE 6.1. The libraries contain multiple burnup-dependent cross-sections for seven PWR fuel designs, with enrichments ranging from 1.5 to 6 wt% {sup 235}U. The burnup range has been extended from the 72 GWd/MTU used in previous versions of the libraries to 90 GWd/MTU. Validation of the libraries using radiochemical assay measurements and decay heat measurements for PWR spent fuel showed good agreement between calculated and experimental data. Verification against detailed TRITON simulations for the considered assembly designs showed that depletion calculations performed in ORIGEN-ARP with the pre-generated libraries provide similar results as obtained with direct TRITON depletion, while greatly reducing the computation time. (authors)

  20. Physics and Algorithm Enhancements for a Validated MCNP/X Monte Carlo Simulation Tool, Phase VII

    Energy Technology Data Exchange (ETDEWEB)

    McKinney, Gregg W [Los Alamos National Laboratory

    2012-07-17

    Currently the US lacks an end-to-end (i.e., source-to-detector) radiation transport simulation code with predictive capability for the broad range of DHS nuclear material detection applications. For example, gaps in the physics, along with inadequate analysis algorithms, make it difficult for Monte Carlo simulations to provide a comprehensive evaluation, design, and optimization of proposed interrogation systems. With the development and implementation of several key physics and algorithm enhancements, along with needed improvements in evaluated data and benchmark measurements, the MCNP/X Monte Carlo codes will provide designers, operators, and systems analysts with a validated tool for developing state-of-the-art active and passive detection systems. This project is currently in its seventh year (Phase VII). This presentation will review thirty enhancements that have been implemented in MCNPX over the last 3 years and were included in the 2011 release of version 2.7.0. These improvements include 12 physics enhancements, 4 source enhancements, 8 tally enhancements, and 6 other enhancements. Examples and results will be provided for each of these features. The presentation will also discuss the eight enhancements that will be migrated into MCNP6 over the upcoming year.

  1. EFEKTIVITAS PENDEKATAN OPEN-ENDED DAN CTL DITINJAU DARI BERPIKIR KREATIF SISWA KELAS VII

    Directory of Open Access Journals (Sweden)

    Yeni Rahmawati ES

    2016-06-01

    Full Text Available This research aimed to describe the effectiveness of the open-ended and CTL approach in terms of creative thinking. This research was quasi-experimental study design with nonequivalent pretest-posttest design goup. The research population was all students in class VII SMP Negeri 1 Sekampung which consisted of five classes. The research sample consisted of two classes is determined randomly. First experimental group was treated the open-ended and experimental group II were treated CTL approach. The one sample t-test carried out to investigate the effectiveness of the open-ended and CTL approach. Data collection techniques used were tests. Engineering tests used to measure student creative thinking on basic competencies: 1 identify the properties of a rectangle, square, trapezoid, parallelogram, rhombus and a kite; 2 calculate the circumference and area of a rectangle up and use it in problem solving both before and after treatment. The results of the research show that the open-ended and CTL approach is effective in terms of creative thinking. Keywords: open-ended, contextual teaching and learning, creative thinking

  2. Standardization of an ELISA test using a recombinant nucleoprotein from the Junin virus as the antigen and serological screening for arenavirus among the population of Nova Xavantina, State of Mato Grosso Padronização de um teste de ELISA utilizando a nucleoproteína recombinante de vírus Junin como antígeno e triagem sorológica para Arenavírus na população de Nova Xavantina, Estado do Mato Grosso

    Directory of Open Access Journals (Sweden)

    Alex Martins Machado

    2010-06-01

    Full Text Available INTRODUCTION: Arenavirus hemorrhagic fever is a severe emerging disease. METHODS: Considering that the levels of antibodies against arenavirus in the Brazilian population are completely unknown, we have standardized an ELISA test for detecting IgG antibodies using a recombinant nucleoprotein from the Junin virus as the antigen. This protein was obtained by inserting the gene of the Junin virus nucleoprotein into the genome of Autographa californica nucleopolyhedrovirus, using the Bac-to-Bac baculovirus expression system. This recombinant baculovirus was used to infect S. frugiperda cells (SF9. RESULTS: The infection resulted in synthesis of high concentrations of recombinant protein. This protein was detected on 12.5% polyacrylamide gel and by means of Western blot. Using the standardized ELISA test, 343 samples from the population of Nova Xavantina were analyzed. We observed that 1.4% of the serum samples (five samples presented antibody titers against arenavirus. CONCLUSIONS: These results show the population studied may present exposure to arenavirus infection.INTRODUÇÃO: A febre hemorrágica por Arenavirus é uma severa doença emergente. MÉTODOS: Considerando que os níveis de anticorpos contra Arenavirus na população brasileira é totalmente desconhecido, nos padronizamos um teste de ELISA para detecção de anticorpos IgG usando uma nucleoproteína recombinante do vírus Junin como antígeno. Esta proteína foi obtida pela inserção do gene da nucleoproteína do vírus Junin no genoma do vírus Autographa californica nucleopolyhedrovirus, utilizando o sistema de expressão em Baculovírus, Bac-To-Bac. Este baculovirus recombinante foi utilizado para infecção de células de S. frugiperda (Sf9. RESULTADOS: A infecção resultou na produção de altas concentrações de proteína recombinante. Esta proteína foi detectada em gel de poliacrilamida 12,5%, e em Western blot. Utilizando o teste de ELISA padronizado, foram analizadas 343

  3. Field evaluation of safety during gestation and horizontal spread of a recombinant differential bovine herpesvirus 1 (BoHV-1 vaccine Avaliação a campo da segurança para vacas prenhes e capacidade de disseminação horizontal de uma vacina diferencial recombinante contra o Herpes-vírus Bovino tipo 1 (BoHV-1

    Directory of Open Access Journals (Sweden)

    Fernando R. Spilki

    2005-03-01

    indicate that, under the conditions of the present study, the gE- vaccine virus did not cause any noticeable harmful effect on pregnant dams and on its offspring and did not spread horizontally among cattle.Infecções pelo herpesvírus bovino tipo 1 (BoHV-1 são importantes causas de doença respiratória, reprodutiva e abortos em bovinos. A vacinação é freqüentemente empregada para minimizar as perdas produzidas pela infecção. Todavia, a imunização de vacas durante a prenhez com algumas vacinas contendo vírus vivo modificado (MLV pode ocasionalmente causar abortos. Em trabalho prévio, nosso grupo desenvolveu uma vacina recombinante de BoHV-1 construída a partir de um isolado brasileiro de BoHV-1 (Franco et al., 2002a do qual o gene que codifica para a glicoproteína E (gE foi artificialmente deletado. Tal recombinante (gE- vem sendo avaliado como vacina diferencial, isto é, capaz de permitir a diferenciação entre animais vacinados e infectados. No presente estudo, o potencial de disseminação do vírus recombinante foi avaliado em um rebanho de gado de corte, em condições de campo. Para tanto, a segurança da vacina gE- quando aplicada durante a prenhez foi avaliada pela inoculação intramuscular de 10(7,4 doses infectantes para 50% dos cultivos celulares (DICC50 do vírus em 22 fêmeas prenhes (14 previamente soronegativas e 8 previamente soropositivas para BoHV-1 em diferentes fases da gestação. Outras 15 vacas prenhes foram mantidas como controles não-vacinados. Não ocorreram abortos, natimortos ou anormalidades fetais em nenhum dos grupos. Soroconversão foi observada nas fêmeas vacinadas previamente soronegativas. Em um segundo experimento, 4 novilhas foram inoculadas pela via intranasal com 10(7,6 DICC50 do vírus recombinante, sendo mantidos em contato com 16 novilhas em uma área de campo, a uma densidade de 1 animal por hectare. Os animais foram monitorados quanto à presença de sinais clínicos; amostras de soro foram coletadas nos dias

  4. Use of Recombinant Factor VIIa in US Military Casualties for a Five-Year Period

    Science.gov (United States)

    2010-08-01

    Recombinant factor VIIa as adjunctive therapy for bleeding control in severely injured trauma patients: two parallel randomized, placebo-controlled...particularly Factor VII level, in order that all of us could help learn the ideal plasma to red cell ratio by science rather than by some gestalt ? Dr. Slate

  5. First human treatment with investigational rhGUS enzyme replacement therapy in an advanced stage MPS VII patient.

    Science.gov (United States)

    Fox, Joyce E; Volpe, Linda; Bullaro, Josephine; Kakkis, Emil D; Sly, William S

    2015-02-01

    Mucopolysaccharidosis type VII (MPS VII, Sly syndrome) is a very rare lysosomal storage disease caused by a deficiency of the enzyme β-glucuronidase (GUS), which is required for the degradation of three glycosaminoglycans (GAGs): dermatan sulfate, heparan sulfate, and chondroitin sulfate. Progressive accumulation of these GAGs in lysosomes leads to increasing dysfunction in numerous tissues and organs. Enzyme replacement therapy (ERT) has been used successfully for other MPS disorders, but there is no approved treatment for MPS VII. Here we describe the first human treatment with recombinant human GUS (rhGUS), an investigational therapy for MPS VII, in a 12-year old boy with advanced stage MPS VII. Despite a tracheostomy, nocturnal continuous positive airway pressure, and oxygen therapy, significant pulmonary restriction and obstruction led to oxygen dependence and end-tidal carbon dioxide (ETCO2) levels in the 60-80mmHg range, eventually approaching respiratory failure (ETCO2 of 100mmHg) and the need for full-time ventilation. Since no additional medical measures could improve his function, we implemented experimental ERT by infusing rhGUS at 2mg/kg over 4h every 2 weeks for 24 weeks. Safety was evaluated by standard assessments and observance for any infusion associated reactions (IARs). Urinary GAG (uGAG) levels, pulmonary function, oxygen dependence, CO2 levels, cardiac valve function, liver and spleen size, and growth velocity were assessed to evaluate response to therapy. rhGUS infusions were well tolerated. No serious adverse events (SAEs) or IARs were observed. After initiation of rhGUS infusions, the patient's uGAG excretion decreased by more than 50%. Liver and spleen size were reduced within 2 weeks of the first infusion and reached normal size by 24 weeks. Pulmonary function appeared to improve during the course of treatment based on reduced changes in ETCO2 after off-ventilator challenges and a reduced oxygen requirement. The patient regained the

  6. ENDF/B-VII.1 Neutron Cross Section Data Testing with Critical Assembly Benchmarks and Reactor Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Kahler, A. [Los Alamos National Laboratory (LANL); Macfarlane, R E [Los Alamos National Laboratory (LANL); Mosteller, R D [Los Alamos National Laboratory (LANL); Kiedrowski, B C [Los Alamos National Laboratory (LANL); Frankle, S C [Los Alamos National Laboratory (LANL); Chadwick, M. B. [Los Alamos National Laboratory (LANL); Mcknight, R D [Argonne National Laboratory (ANL); Lell, R M [Argonne National Laboratory (ANL); Palmiotti, G [Idaho National Laboratory (INL); Hiruta, h [Idaho National Laboratory (INL); Herman, Micheal W [Brookhaven National Laboratory (BNL); Arcilla, r [Brookhaven National Laboratory (BNL); Mughabghab, S F [Brookhaven National Laboratory (BNL); Sublet, J C [Culham Science Center, Abington, UK; Trkov, A. [Jozef Stefan Institute, Slovenia; Trumbull, T H [Knolls Atomic Power Laboratory; Dunn, Michael E [ORNL

    2011-01-01

    The ENDF/B-VII.1 library is the latest revision to the United States' Evaluated Nuclear Data File (ENDF). The ENDF library is currently in its seventh generation, with ENDF/B-VII.0 being released in 2006. This revision expands upon that library, including the addition of new evaluated files (was 393 neutron files previously, now 423 including replacement of elemental vanadium and zinc evaluations with isotopic evaluations) and extension or updating of many existing neutron data files. Complete details are provided in the companion paper [1]. This paper focuses on how accurately application libraries may be expected to perform in criticality calculations with these data. Continuous energy cross section libraries, suitable for use with the MCNP Monte Carlo transport code, have been generated and applied to a suite of nearly one thousand critical benchmark assemblies defined in the International Criticality Safety Benchmark Evaluation Project's International Handbook of Evaluated Criticality Safety Benchmark Experiments. This suite covers uranium and plutonium fuel systems in a variety of forms such as metallic, oxide or solution, and under a variety of spectral conditions, including unmoderated (i.e., bare), metal reflected and water or other light element reflected. Assembly eigenvalues that were accurately predicted with ENDF/B-VII.0 cross sections such as unrnoderated and uranium reflected (235)U and (239)Pu assemblies, HEU solution systems and LEU oxide lattice systems that mimic commercial PWR configurations continue to be accurately calculated with ENDF/B-VII.1 cross sections, and deficiencies in predicted eigenvalues for assemblies containing selected materials, including titanium, manganese, cadmium and tungsten are greatly reduced. Improvements are also confirmed for selected actinide reaction rates such as (236)U; (238,242)Pu and (241,243)Am capture in fast systems. Other deficiencies, such as the overprediction of Pu solution system critical

  7. ENDF/B-VII.1 Nuclear Data for Science and Technology: Cross Sections, Covariances, Fission Product Yields and Decay Data

    Energy Technology Data Exchange (ETDEWEB)

    Chadwick, M. B. [Los Alamos National Laboratory (LANL); Herman, Micheal W [Brookhaven National Laboratory (BNL); Oblozinsky, Pavel [Brookhaven National Laboratory (BNL); Dunn, Michael E [ORNL; Danon, Y. [Rensselaer Polytechnic Institute (RPI); Kahler, A. [Los Alamos National Laboratory (LANL); Smith, Donald L. [Argonne National Laboratory (ANL); Pritychenko, B [Brookhaven National Laboratory (BNL); Arbanas, Goran [ORNL; Arcilla, r [Brookhaven National Laboratory (BNL); Brewer, R [Los Alamos National Laboratory (LANL); Brown, D A [Brookhaven National Laboratory (BNL); Capote, R. [International Atomic Energy Agency (IAEA); Carlson, A. D. [National Institute of Standards and Technology (NIST); Cho, Y S [Korea Atomic Energy Research Institute; Derrien, Herve [ORNL; Guber, Klaus H [ORNL; Hale, G. M. [Los Alamos National Laboratory (LANL); Hoblit, S [Brookhaven National Laboratory (BNL); Holloway, Shannon T. [Los Alamos National Laboratory (LANL); Johnson, T D [Brookhaven National Laboratory (BNL); Kawano, T. [Los Alamos National Laboratory (LANL); Kiedrowski, B C [Los Alamos National Laboratory (LANL); Kim, H [Korea Atomic Energy Research Institute; Kunieda, S [Los Alamos National Laboratory (LANL); Larson, Nancy M [ORNL; Leal, Luiz C [ORNL; Lestone, J P [Los Alamos National Laboratory (LANL); Little, R C [Los Alamos National Laboratory (LANL); Mccutchan, E A [Brookhaven National Laboratory (BNL); Macfarlane, R E [Los Alamos National Laboratory (LANL); MacInnes, M [Los Alamos National Laboratory (LANL); Matton, C M [Lawrence Livermore National Laboratory (LLNL); Mcknight, R D [Argonne National Laboratory (ANL); Mughabghab, S F [Brookhaven National Laboratory (BNL); Nobre, G P [Brookhaven National Laboratory (BNL); Palmiotti, G [Idaho National Laboratory (INL); Palumbo, A [Brookhaven National Laboratory (BNL); Pigni, Marco T [ORNL; Pronyaev, V. G. [Institute of Physics and Power Engineering (IPPE), Obninsk, Russia; Sayer, Royce O [ORNL; Sonzogni, A A [Brookhaven National Laboratory (BNL); Summers, N C [Lawrence Livermore National Laboratory (LLNL); Talou, P [Los Alamos National Laboratory (LANL); Thompson, I J [Lawrence Livermore National Laboratory (LLNL); Trkov, A. [Jozef Stefan Institute, Slovenia; Vogt, R L [Lawrence Livermore National Laboratory (LLNL); Van der Marck, S S [Nucl Res & Consultancy Grp, Petten, Netherlands; Wallner, A [University of Vienna, Austria; White, M C [Los Alamos National Laboratory (LANL); Wiarda, Dorothea [ORNL; Young, P C [Los Alamos National Laboratory (LANL)

    2011-01-01

    The ENDF/B-VII.1 library is our latest recommended evaluated nuclear data file for use in nuclear science and technology applications, and incorporates advances made in the five years since the release of ENDF/B-VII.0. These advances focus on neutron cross sections, covariances, fission product yields and decay data, and represent work by the US Cross Section Evaluation Working Group (CSEWG) in nuclear data evaluation that utilizes developments in nuclear theory, modeling, simulation, and experiment. The principal advances in the new library are: (1) An increase in the breadth of neutron reaction cross section coverage, extending from 393 nuclides to 423 nuclides; (2) Covariance uncertainty data for 190 of the most important nuclides, as documented in companion papers in this edition; (3) R-matrix analyses of neutron reactions on light nuclei, including isotopes of He; Li, and Be; (4) Resonance parameter analyses at lower energies and statistical high energy reactions for isotopes of Cl; K; Ti, V, Mn, Cr, Ni, Zr and W; (5) Modifications to thermal neutron reactions on fission products (isotopes of Mo, Tc, Rh, Ag, Cs, Nd, Sm, Eu) and neutron absorber materials (Cd, Gd); (6) Improved minor actinide evaluations for isotopes of U, Np, Pu, and Am (we are not making changes to the major actinides (235,238)U and (239)Pu at this point, except for delayed neutron data and covariances, and instead we intend to update them after a further period of research in experiment and theory), and our adoption of JENDL-4.0 evaluations for isotopes of Cm, Bk, Cf, Es; Fm; and some other minor actinides; (7) Fission energy release evaluations; (8) Fission product yield advances for fission-spectrum neutrons and 14 MeV neutrons incident on (239)Pu; and (9) A new decay data sublibrary. Integral validation testing of the ENDF/B-VII.1 library is provided for a variety of quantities: For nuclear criticality, the VII.1 library maintains the generally-good performance seen for VII.0 for a wide

  8. Transition of an X-ray binary to the hard ultraluminous state in the blue compact dwarf galaxy VII Zw 403

    CERN Document Server

    Brorby, Matthew; Feng, Hua

    2015-01-01

    We examine the X-ray spectra of VII Zw 403, a nearby low-metallicity blue compact dwarf (BCD) galaxy. The galaxy has been observed to contain an X-ray source, likely a high mass X-ray binary (HMXB), with a luminosity of 1.3-23x10^38 erg s^-1 in the 0.3-8 keV energy range. A new Suzaku observation shows a transition to a luminosity of 1.7x10^40 erg s^-1 [0.3-8 keV], higher by a factor of 7-130. The spectra from the high flux state are hard, best described by a disk plus Comptonization model, and exhibit curvature at energies above 5 keV. This is consistent with many high-quality ultraluminous X-ray source spectra which have been interpreted as stellar mass black holes (StMBH) accreting at super-Eddington rates. However, this lies in contrast to another HMXB in a low-metallicity BCD, I Zw 18, that exhibits a soft spectrum at high flux, similar to Galactic black hole binaries and has been interpreted as a possible intermediate mass black hole. Determining the spectral properties of HMXBs in BCDs has important im...

  9. Contextos cerámicos de los siglos V-VII en Cartagena

    Directory of Open Access Journals (Sweden)

    Ramallo Asensio, Sebastián F.

    1996-12-01

    Full Text Available The excavations realized in the Roman theatre of Cartagena have documented a wide stratigraphie sequence with covers levels from IInd century B.C. to today. The material contexts of Vth to VIIth B.C. are specially interesting because they were associated with considerable removals of the town planning. The construction of a comercial site in the second half of the Vth century, markets and stores, built on a big exedra with portico and 15 divisions in line, represents the total use of the theatre, the structures of which became fossilized, in part, in a new shape. A village of Byzantin age was built over both constructions, characterized with quadrangular rooms which alternates with opened public spaces and irregular streets adjusted to the artificial and inclined topography dependent on the precedent constructions.Las excavaciones realizadas en el teatro romano de Cartagena han documentado una amplia secuencia estratigráfica que abarca desde niveles del siglo II a. de C. hasta nuestros días. Especialmente interesantes son los contextos materiales de los siglos V al VII, asociados a profundas remociones de carácter urbanístico. La construcción en la segunda mitad del siglo V de un complejo comercial, mercado/almacén, estructurado sobre una gran exedra porticada y una serie de 15 compartimentos alineados, representa la amortización total del edificio del teatro cuyas estructuras en parte fosiliza el nuevo conjunto. Encima de ambas construcciones se construyó un barrio de época bizantina caracterizado por habitaciones de forma cuadrangular que alternan con espacios comunes abiertos y calles de trazado irregular adaptadas a la topografía artificial y en pendiente impuesta por las construcciones precedentes.

  10. A novel family VII esterase with industrial potential from compost metagenomic library

    Directory of Open Access Journals (Sweden)

    Oh Tae-Kwang

    2011-05-01

    Full Text Available Abstract Background Among the vast microbial genomic resources now available, most microbes are unculturable in the laboratory. A culture-independent metagenomic approach is a novel technique that circumvents this culture limitation. For the screening of novel lipolytic enzymes, a metagenomic library was constructed from compost, and the clone of estCS2 was selected for lipolytic properties on a tributyrin-containing medium. Results The estCS2 sequence encodes a protein of 570 amino acid residues, with a predicted molecular mass of 63 kDa, and based on amino acid identity it most closely matches (45% the carboxylesterase from Haliangium ochraceum DSM 14365. EstCS2 belong to family VII, according to the lipolytic enzyme classification proposed by Arpigny and Jaeger, and it retains the catalytic triad Ser245-Glu363-His466 that is typical of an α/β hydrolase. The Ser245 residue in the catalytic triad of EstCS2 is located in the consensus active site motif GXSXG. The EstCS2 exhibits strong activity toward p-nitrophenyl caproate (C6, and it is stable up to 60°C with an optimal enzymatic activity at 55°C. The maximal activity is observed at pH 9, and it remains active between pH 6-10. EstCS2 shows remarkable stability in up to 50% (v/v dimethyl sulfoxide (DMSO or dimethylformamide (DMF. The enzyme has the ability to cleave sterically hindered esters of tertiary alcohol, as well as to degrade polyurethanes, which are widely used in various industries. Conclusions The high stability of EstCS2 in organic solvents and its activity towards esters of ketoprofen and tertiary alcohols, and in polyurethane suggests that it has potential uses for many applications in biotransformation and bioremediation.

  11. Basement membrane zone remodeling during appendageal development in human fetal skin. The absence of type VII collagen is associated with gelatinase-A (MMP2) activity.

    Science.gov (United States)

    Karelina, T V; Bannikov, G A; Eisen, A Z

    2000-02-01

    Epithelial cell adhesion, migration, and differentiation are controlled by interactions at the basement membrane zone (BMZ). Type VII collagen is the major collagenous component of anchoring fibrils that are essential for the attachment of the epidermis to the dermis. Gelatinase A (MMP-2) is believed to be necessary for the degradation of type VII collagen. In this study we have examined the in vivo distribution of type VII collagen and gelatinase A (Gel A) in the developing human epidermis and its appendages. At 13-15 wk of gestation a marked decrease in type VII collagen immunoreactivity was seen in the BMZ surrounding invading appendageal buds; however, type VII collagen mRNA was strongly expressed in the budding epidermal keratinocytes adjacent to the BMZ. At these stages, Gel A-positive mesenchymal-like cells were found scattered throughout the stroma with numerous Gel A-containing cells in direct contact with the developing appendageal buds. In situ zymography was used to show Gel A-activity in vivo. Gel A-mediated lysis was present at the interface between the appendageal buds and the underlying BMZ. By 20-25 wk of gestational age, immunostaining for type VII collagen protein was absent from the BMZ surrounding the distal portion of invading appendageal epithelial cords of both hair follicles and sweat glands. In contrast, type VII collagen mRNA was present in the basal keratinocytes adjacent to the BMZ surrounding the distal portion of these invading appendageal epithelial cords. At these stages Gel A-positive cells were present in the stroma directly adjacent to the distal portion of developing appendageal cords that lacked type VII collagen. In situ zymography showed zones of Gel A-mediated stromal lysis at the distal portion of developing appendageal cords. Interestingly, no differences were seen in the distribution of type IV collagen in the BMZ of both budding and resting fetal epidermis. These observations suggest that the absence of type VII collagen

  12. Bovine adenovirus 3 core protein precursor pVII localizes to mitochondria, and modulates ATP synthesis, mitochondrial Ca2+ and mitochondrial membrane potential.

    Science.gov (United States)

    Anand, Sanjeev K; Gaba, Amit; Singh, Jaswant; Tikoo, Suresh K

    2014-02-01

    Viruses modulate the functions of mitochondria by translocating viral proteins to the mitochondria. Subcellular fractionation and sensitivity to proteinase K/Triton X-100 treatment of mitochondrial fractions of bovine adenovirus (BAdV)-3-infected/transfected cells suggested that core protein pVII localizes to the mitochondria and contains a functional mitochondrial localization signal. Moreover, mitochondrial localization of BAdV-3 pVII appears to help in the retention of mitochondrial Ca(2+), inducing a significant increase in the levels of ATP and maintaining the mitochondrial membrane potential (MMP) in transfected cells. In contrast, mitochondrial localization of BAdV-3 pVII has no significant effect on the levels of cytoplasmic Ca(2+) and reactive oxygen species production in the transfected cells. Consistent with these results, expression of pVII in transfected cells treated with staurosporine decreased significantly the activation of caspase-3. Our results suggested that BAdV-3 pVII localizes to mitochondria, and interferes with apoptosis by inhibiting loss of the MMP and by increasing mitochondrial Ca(2+) and ATP production.

  13. Magnetic anomaly patterns over crustal blocks of the King Edward VII Peninsula, Marie Byrd Land, West Antarctica

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    M. Spano

    2000-06-01

    Full Text Available Within the framework of the GITARA II project an aeromagnetic survey was performed during the GANOVEXVII expedition (1992/1993 over the King Edward VII Peninsula in northwestern Marie Byrd Land (West Antarctica. This region which may represent the eastern flank of the Ross Sea rift system had previously been explored only at reconnaissance level. New total field and upward continued (10 km magnetic anomaly maps are produced and interpreted here to map and discuss the crustal structure of the Edward VII Peninsula. Tworound-shaped, high-amplitude magnetic anomalies are recognised over the Alexandra Mountains block. The anomalies are difficult to interpret since susceptibility data indicate the prevalence of non-magnetic rocks at the surface. A possible interpretation is that the anomalies are due to Cretaceous mafic intrusives distinct from weakly magnetic Byrd Coast Granite of the adjacent Rockefeller Mountains block. Alternatively the anomalies could be related to buried pluton-sized Devonian Ford Granodiorite intruded by dikes. If Cretaceous in age, the former intrusives revealed from the magnetics could also be responsible for contact metamorphism of the adjacent Alexandra Mountains migmatites. Lower amplitude circular anomalies over the Central Plateau and Prestrud Inlet are likely to be caused by unexposed Devonian Ford Granodiorite which crops out in the Ford Ranges. Elongated high-frequency anomalies of the Sulzberger Bay are similar to those recognised over seismically constrained Cenozoic rift-related volcanics of the Ross Sea. A broad magnetic low over the Sulzberger Ice Shelf may be indicative of a fault bounded graben-like basin with sedimentary infill. Overall recognition of magnetic anomaly patterns and trends reveals segmentation of the Edward VII Peninsula and of the adjacent marine areas in distinct crustal blocks. Faults may separate these blocks and they are interpreted to reflect multiple Cretaceous and maybe Cenozoic crustal

  14. Comparison of student's learning achievement through realistic mathematics education (RME) approach and problem solving approach on grade VII

    Science.gov (United States)

    Ilyas, Muhammad; Salwah

    2017-02-01

    The type of this research was experiment. The purpose of this study was to determine the difference and the quality of student's learning achievement between students who obtained learning through Realistic Mathematics Education (RME) approach and students who obtained learning through problem solving approach. This study was a quasi-experimental research with non-equivalent experiment group design. The population of this study was all students of grade VII in one of junior high school in Palopo, in the second semester of academic year 2015/2016. Two classes were selected purposively as sample of research that was: year VII-5 as many as 28 students were selected as experiment group I and VII-6 as many as 23 students were selected as experiment group II. Treatment that used in the experiment group I was learning by RME Approach, whereas in the experiment group II by problem solving approach. Technique of data collection in this study gave pretest and posttest to students. The analysis used in this research was an analysis of descriptive statistics and analysis of inferential statistics using t-test. Based on the analysis of descriptive statistics, it can be concluded that the average score of students' mathematics learning after taught using problem solving approach was similar to the average results of students' mathematics learning after taught using realistic mathematics education (RME) approach, which are both at the high category. In addition, It can also be concluded that; (1) there was no difference in the results of students' mathematics learning taught using realistic mathematics education (RME) approach and students who taught using problem solving approach, (2) quality of learning achievement of students who received RME approach and problem solving approach learning was same, which was at the high category.

  15. Membrane interactions and self‐association of components of the Ess/Type VII secretion system of Staphylococcus aureus

    OpenAIRE

    Jäger, Franziska; Zoltner, Martin; Kneuper, Holger; Hunter, William N.; Palmer, Tracy

    2016-01-01

    The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected i...

  16. Changes of androgen receptor expression in stages VII-VIII seminiferous tubules of rat testis after exposure to methamphetamine

    OpenAIRE

    Sutita Nudmamud-Thanoi; Nareelak Tangsrisakda; Samur Thanoi

    2016-01-01

    Methamphetamine (METH) is toxic not only to the central nervous system but also to the reproductive system. This study aimed to investigate the effect of METH administration on androgen receptor (AR) expression in the seminiferous tubules (stages VII-VIII) and Leydig cells. 24 Sprague-Dawley rats were equally divided into 4 groups; control, acute doseMETH binge (AB-METH), escalating dose-METH (ED-METH) and escalating dose-METH binge (ED-METH binge) groups. The expressions of AR we...

  17. An effective treatment of severe intractable bleeding after valve repair by one single dose of activated recombinant factor VII

    NARCIS (Netherlands)

    Hendriks, HGD; van der Maaten, JMAA; de Wolf, J; Slooff, MJH; van der Meer, J; Waterbolk, T

    Excessive bleeding (>2 L) after cardiac surgery is encountered in 3% to 7% of patients (1), and necessitates reexploration in 3.6% (2) to 4.2% (3) of cases. A variety of hemostatic defects may contribute and are probably induced by cardiopulmonary bypass (CPB), i.e., impaired platelet function,

  18. 111Indium Labelling of Recombinant Activated Coagulation Factor VII: In Vitro and Preliminary In Vivo Studies in Healthy Rats

    Directory of Open Access Journals (Sweden)

    Amarnadh Nalla

    2012-01-01

    Full Text Available The aim of this study is to investigate whether 111Indium-labelled recombinant FVIIa (rFVIIa could be a potential radiopharmaceutical for localization of bleeding sources. DTPA-conjugated rFVIIa was radiolabelled with 111In chloride. In vitro binding efficiency of 111In-DTPA-rFVIIa to F1A2-Mab-sepharose was 99% in buffer, while it was 88–82% in serum. The binding efficiency of 111In-DTPA-rFVIIa to TF (1–209-sepharose was 48% in buffer whereas 39%–36% in serum, respectively. In vivo experiment was conducted in healthy rats, and gamma camera images were taken immediately after iv. administration of 1.6–1.8 MBq 111In-DTPA-rFVIIa up to 120–130 min. Five min after administration of 111In-DTPA-rFVIIa, percentage of 111In activity was 6.0% in the cardiac region and 24.5% in the liver region. After 2 hours activity was decreased to 3.3% in heart while it had increased to 42.0% in the liver. The 111In-DTPA-rFVIIa might be a potential radiopharmaceutical for visualisation of tissues with significant TF expression such as acute bleeding lesions in the gastrointestinal tract.

  19. Lower extremity compartment syndrome in the setting of iliofemoral deep vein thrombosis, phlegmasia cerulea dolens and factor VII deficiency.

    Science.gov (United States)

    Abdul, Wahid; Hickey, Ben; Wilson, Chris

    2016-04-25

    Acute compartment syndrome requires urgent fasciotomies to prevent irreversible muscle damage. We present a case of massive iliofemoral deep vein thrombosis (DVT) presenting as acute compartment syndrome. A healthy 21-year-old man presented with a 2-day history of worsening left leg pain with swelling and bluish discolouration. Clinical diagnosis of compartment syndrome secondary to phlegmasia cerulea dolens (PCD) was made and he underwent emergency fasciotomies. Postoperative venous duplex confirmed a massive iliofemoral DVT and intravenous heparin was started. Following skin grafting, the patient made a good recovery. Massive iliofemoral DVT is an uncommon cause of compartment syndrome and has been reported in lower limbs, secondary to PCD. Failure to treat early carries a high degree of morbidity, with amputation rates up to 50% and mortality rates between 25% and 40%. It is important to recognise compartment syndrome as an acute presentation of PCD. Urgent fasciotomies can prevent limb amputation and mortality. 2016 BMJ Publishing Group Ltd.

  20. Factor VII Activating Protease Polymorphism (G534E) Is Associated with Increased Risk for Stroke and Mortality.

    Science.gov (United States)

    Trompet, Stella; Pons, Douwe; Kanse, Sandip M; de Craen, Anton J M; Ikram, M Arfan; Verschuren, Jeffrey J W; Zwinderman, Aeilko H; Doevendans, Pieter A F M; Tio, René A; de Winter, Robbert J; Slagboom, P Eline; Westendorp, Rudi G J; Jukema, J Wouter

    2011-01-01

    Introduction. The FSAP-Marburg I polymorphism (1704G > A), which reduces FSAP activity, is associated with late complications of carotid stenosis in humans. Therefore, this study examines the influence of the Marburg I polymorphism and the closely linked Marburg II polymorphism (1280G > C) on various cardiovascular outcomes in two large independent study populations. Methods. The two Marburg polymorphisms in the HABP2 gene encoding FSAP were genotyped in a large population of elderly patients at risk for vascular disease (the PROSPER-study, n = 5804) and in a study population treated with a percutaneous coronary intervention (the GENDER-study, n = 3104). Results. In the PROSPER study, the Marburg I polymorphism was associated with an increased risk of clinical stroke (HR: 1.60, 95% CI: 1.13-2.28) and all-cause mortality (HR: 1.33, 95% CI: 1.04-1.71). In the GENDER study carriers of this variant seemed at lower risk of developing restenosis (HR: 0.59, 95% CI: 0.34-1.01). The Marburg II polymorphism showed similar but weaker results. Conclusion. The increase in stroke risk in Marburg I carriers could be due to differential effects on smooth muscle cells and on matrix metalloproteinases, thereby influencing plaque stability. The possible protective effect on restenosis could be the result of reduced activation of zymogens, which are involved in hemostasis and matrix remodeling.

  1. Factor VII Activating Protease Polymorphism (G534E Is Associated with Increased Risk for Stroke and Mortality

    Directory of Open Access Journals (Sweden)

    Stella Trompet

    2011-01-01

    Full Text Available Introduction. The FSAP-Marburg I polymorphism (1704G > A, which reduces FSAP activity, is associated with late complications of carotid stenosis in humans. Therefore, this study examines the influence of the Marburg I polymorphism and the closely linked Marburg II polymorphism (1280G > C on various cardiovascular outcomes in two large independent study populations. Methods. The two Marburg polymorphisms in the HABP2 gene encoding FSAP were genotyped in a large population of elderly patients at risk for vascular disease (the PROSPER-study, n=5804 and in a study population treated with a percutaneous coronary intervention (the GENDER-study, n=3104. Results. In the PROSPER study, the Marburg I polymorphism was associated with an increased risk of clinical stroke (HR: 1.60, 95% CI: 1.13–2.28 and all-cause mortality (HR: 1.33, 95% CI: 1.04–1.71. In the GENDER study carriers of this variant seemed at lower risk of developing restenosis (HR: 0.59, 95% CI: 0.34–1.01. The Marburg II polymorphism showed similar but weaker results. Conclusion. The increase in stroke risk in Marburg I carriers could be due to differential effects on smooth muscle cells and on matrix metalloproteinases, thereby influencing plaque stability. The possible protective effect on restenosis could be the result of reduced activation of zymogens, which are involved in hemostasis and matrix remodeling.

  2. Uso do estimulante de colônia de granulócitos nas neutropenias em cães e gatos Granulocyte colony-stimulating factor use in neutropenias in dogs and cats

    Directory of Open Access Journals (Sweden)

    Cynthia de Assumpção Lucidi

    2007-06-01

    Full Text Available As neutropenias persistentes podem ser decorrentes de alterações na granulopoiese, causadas por efeitos supressivos ou tóxicos à medula óssea, predispõem o paciente a infecções comprometendo sua sobrevida. As neutropenias intensas decorrentes de toxicidade por quimioterápicos podem requerer a suspensão temporária ou permanente do medicamento, podendo gerar resistência das células neoplásicas ao tratamento. O uso de fatores de crescimento hematopoiético recombinantes em animais tem aumentado muito nos últimos anos, devido a sua crescente disponibilidade na medicina humana. O fator estimulante de colônia para granulócitos recombinante humano (rhG-CSF age aumentando o número de neutrófilos circulantes e possui grande potencial para amenizar ou reverter quadros de neutropenia associada a condições de mielotoxicidade e mielosupressão em cães e gatos.Persistent neutropenias can occur after granulopoiesis disturbances caused by myelosupressive or myelotoxic effects, and predispose patients to infections and impairs their survival. Furthermore, severe chemotherapy-induced neutropenias must require temporary or definitive treatment interruption, what may lead to drug-resistance of neoplastic cells. The use of recombinant stem cell factors in animals has been increasing due to its bigger disponibility for human beings. The human recombinant granulocyte colony-stimulating factor (rhG-CSF increases neutrophil numbers in peripheral blood and has great potential to alleviate or revert myelotoxic or myelosupression neutropenias in dogs and cats.

  3. Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep Comparação entre ELISA baseado no antígeno recombinante NcSRS2 e RIFI para detecção de anticorpos de Neospora caninum em ovinos

    Directory of Open Access Journals (Sweden)

    Renato Andreotti

    2009-06-01

    Full Text Available Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05. Serological analysis showed that HisG tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep.Neospora caninum é um parasito Apicomplexa que pode causar abortos e é reconhecido como agente importante responsável por perdas econômicas e reprodutivas. Este estudo avaliou a proteína recombinante NcSRS2 como antígeno para ELISA indireto na determinação de resposta imune para N. caninum em ovinos. 441 amostras de soro foram analisadas por IFAT e ELISA indireto com rNcSRS2 e ambos os testes revelaram comportamento similar. A sensibilidade e especificidade de ELISA indireto foram 98,6 e 98,3%, respectivamente. O índice kappa mostrou uma concordância entre os dois testes com valor de 0,98, que é considerado excelente. Prevalências de 30,8 e 32,0% detectadas por IFAT e ELISA indireto, respectivamente, mostraram que os testes não diferiram significativamente nesse aspecto (P

  4. Somatotropina bovina recombinante (rBST nos aspectos hematológicos e metabólitos do sangue de novilhas (½ Nelore x ½ Red Angus em confinamento Recombinant bovine somatotropin (rBST on hematologic aspects and metabolites of heifers (½ Nellore x ½ Red Angus blood, in feedlot

    Directory of Open Access Journals (Sweden)

    Ivanor Nunes do Prado

    2003-04-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da somatotropina bovina recombinante (rBST - análogo do BST, obtido comercialmente pela técnica do DNA recombinante, sobre os aspectos hematológicos (hematócrito, eritrócitos, hemoglobina, leucócitos, neutrófilos, eosinófilos, linfócitos e monócitos e metabólitos (glicose, insulina, IGF-I, triglicérides, colesterol total e uréia do sangue de novilhas confinadas. Foram utilizadas 24 novilhas mestiças (½ Nelore x ½ Red Angus, com aproximadamente 18 meses de idade e peso médio 255 kg. Os animais foram alimentados com uma dieta contendo silagem de milho como volumoso e polpa de citrus peletizada e farelo de soja, como concentrado, durante 84 dias. Essa dieta foi utilizada para os três tratamentos, que se diferenciaram pela aplicação de 250 mg de rBST, por via subcutânea, na fossa ísqueo-retal, onde: 1 controle (aplicação de dois mL de solução salina; 2 dose única e 3 uma dose a cada 14 dias. Os animais foram distribuídos em delineamento inteiramente casualizado, com três tratamentos e oito repetições. Coleta de sangue foi realizada no início do experimento e nos dias 28, 56 e 84 do experimento, para as determinações dos aspectos hematológicos e metabólitos. Não houve influência da aplicação de rBST sobre os aspectos hematológicos do sangue no início e final do experimento. Todavia, independentemente do tratamento, o dia de coleta teve um efeito linear positivo sobre os níveis de hematócrito, linear negativo sobre os níveis de glicose e triglicérides e quadrático positivo sobre os níveis de IGF-I e uréia. Ainda, não foi observado efeito do dia de coleta sobre os níveis de insulina e colesterol total.This work was carried out to evaluate the recombinant bovine somatotropin effect (rBST - BST analogous, commercially obtained by recombinant DNA technique, on hematological aspects (hematocrit, erythrocyte, hemoglobin, leukocyte, neutrophil, eosinophil

  5. Experimental infection of rabbits with a recombinant bovine herpesvirus type 5 (BoHV-5 gI, gE and US9-negative Infecção experimental de coelhos com um recombinante do herpesvírus bovino tipo 5 defectivo na gI, gE e US9

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    Alessandra D'Avila Silva

    2009-11-01

    Full Text Available Bovine herpesvirus type 5 (BoHV-5 is a major cause of viral meningoencephalitis in cattle. The expression of different viral proteins has been associated with BoHV-5 neuropathogenesis. Among these, gI, gE and US9 have been considered essential for the production of neurological disease in infected animals. To evaluate the role of gI, gE and US9 in neurovirulence, a recombinant from which the respective genes were deleted (BoHV-5 gI-/gE-/US9- was constructed and inoculated in rabbits of two age groups (four and eight weeks-old. When the recombinant virus was inoculated through the paranasal sinuses of four weeks-old rabbits, neurological disease was observed and death was the outcome in 4 out of 13 (30.7 % animals, whereas clinical signs and death were observed in 11/13 (84.6% of rabbits infected with the parental virus. In eight weeks-old rabbits, the BoHV-5 gI-/gE-/US9- did not induce clinically apparent disease and could not be reactivated after dexamethasone administration, whereas wild type BoHV-5 caused disease in 55.5% of the animals and was reactivated. These findings reveal that the simultaneous deletion of gI, gE and US9 genes did reduce but did not completely abolish the neurovirulence of BoHV-5 in rabbits, indicating that other viral genes may also play a role in the induction of neurological disease.O herpesvírus bovino tipo 5 é uma das principais causas de meningoencefalite viral em bovinos. A expressão de diferentes proteínas virais tem sido associada à neuropatogenia do BoHV-5. Entre estas, a gI, gE e US9 têm sido consideradas essenciais para a indução de sinais neurológicos nos animais infectados. Para avaliar o papel das proteínas gI, gE e US9 na neurovirulência, construiu-se um recombinante no qual os genes que codificam estas proteínas foram deletados, denominado BoHV-5 gI-/gE-/US9-. Este vírus foi inoculado em coelhos de idades diferentes (quatro e oito semanas de idade. Quando o vírus recombinante foi inoculado

  6. Stellar laboratories. VII. New Kr IV - VII oscillator strengths and an improved spectral analysis of the hot, hydrogen-deficient DO-type white dwarf RE0503-289

    CERN Document Server

    Rauch, T; Hoyer, D; Werner, K; Richter, P; Kruk, J W; Demleitner, M

    2016-01-01

    For the spectral analysis of high-resolution and high-signal-to-noise (S/N) spectra of hot stars, state-of-the-art non-local thermodynamic equilibrium (NLTE) model atmospheres are mandatory. These are strongly dependent on the reliability of the atomic data that is used for their calculation. New of Kr IV - VII oscillator strengths for a large number of lines allow to construct more detailed model atoms for our NLTE model-atmosphere calculations. This enables us to search for additional Kr lines in observed spectra and to improve Kr abundance determinations. We calculated Kr IV - VII oscillator strengths to consider radiative and collisional bound-bound transitions in detail in our NLTE stellar-atmosphere models for the analysis of Kr lines exhibited in high-resolution and high-S/N ultraviolet (UV) observations of the hot white dwarf RE 0503-289. We reanalyzed the effective temperature and surface gravity and determined Teff = 70 000 +/- 2000 K and log (g / cm/s**2) = 7.5 +/- 0.1. We newly identified ten Kr V...

  7. Stellar laboratories. VIII. New Zr IV - VII, Xe IV - V, and Xe VII oscillator strengths and the Al, Zr, and Xe abundances in the hot white dwarfs G191-B2B and RE0503-289

    CERN Document Server

    Rauch, T; Quinet, P; Loebling, L; Hoyer, D; Werner, K; Kruk, J W; Demleitner, M

    2016-01-01

    For the spectral analysis of high-resolution and high-signal-to-noise spectra of hot stars, state-of-the-art non-local thermodynamic equilibrium (NLTE) model atmospheres are mandatory. These are strongly dependent on the reliability of the atomic data that is used for their calculation. To search for Zr and Xe lines in the ultraviolet (UV) spectra of G191-B2B and RE0503-289, new Zr IV-VII, Xe IV-V, and Xe VIII oscillator strengths were calculated. This allows for the first time, determination of the Zr abundance in white dwarf (WD) stars and improvement of the Xe abundance determinations. We calculated Zr IV-VII, Xe IV-V, and Xe VIII oscillator strengths to consider radiative and collisional bound-bound transitions of Zr and Xe in our NLTE stellar-atmosphere models for the analysis of their lines exhibited in UV observations of the hot WDs G191-B2B and RE0503-289. We identified one new Zr IV, 14 new Zr V, and ten new Zr VI lines in the spectrum of RE0503-289. Zr was detected for the first time in a WD. We mea...

  8. Stellar laboratories . VIII. New Zr iv-vii, Xe iv-v, and Xe vii oscillator strengths and the Al, Zr, and Xe abundances in the hot white dwarfs G191-B2B and RE 0503-289

    Science.gov (United States)

    Rauch, T.; Gamrath, S.; Quinet, P.; Löbling, L.; Hoyer, D.; Werner, K.; Kruk, J. W.; Demleitner, M.

    2017-03-01

    Context. For the spectral analysis of high-resolution and high-signal-to-noise spectra of hot stars, state-of-the-art non-local thermodynamic equilibrium (NLTE) model atmospheres are mandatory. These are strongly dependent on the reliability of the atomic data that is used for their calculation. Aims: To search for zirconium and xenon lines in the ultraviolet (UV) spectra of G191-B2B and RE 0503-289, new Zr iv-vii, Xe iv-v, and Xe vii oscillator strengths were calculated. This allows, for the first time, determination of the Zr abundance in white dwarf (WD) stars and improvement of the Xe abundance determinations. Methods: We calculated Zr iv-vii, Xe iv-v, and Xe vii oscillator strengths to consider radiative and collisional bound-bound transitions of Zr and Xe in our NLTE stellar-atmosphere models for the analysis of their lines exhibited in UV observations of the hot WDs G191-B2B and RE 0503-289. Results: We identified one new Zr iv, 14 new Zr v, and ten new Zr vi lines in the spectrum of RE 0503-289. Zr was detected for the first time in a WD. We measured a Zr abundance of -3.5 ± 0.2 (logarithmic mass fraction, approx. 11 500 times solar). We identified five new Xe vi lines and determined a Xe abundance of -3.9 ± 0.2 (approx. 7500 times solar). We determined a preliminary photospheric Al abundance of -4.3 ± 0.2 (solar) in RE 0503-289. In the spectra of G191-B2B, no Zr line was identified. The strongest Zr iv line (1598.948 Å) in our model gave an upper limit of -5.6 ± 0.3 (approx. 100 times solar). No Xe line was identified in the UV spectrum of G191-B2B and we confirmed the previously determined upper limit of -6.8 ± 0.3 (ten times solar). Conclusions: Precise measurements and calculations of atomic data are a prerequisite for advanced NLTE stellar-atmosphere modeling. Observed Zr iv-vi and Xe vi-vii line profiles in the UV spectrum of RE 0503-289 were simultaneously well reproduced with our newly calculated oscillator strengths. Based on observations

  9. Remineralizing efficacy of silver diamine fluoride and glass ionomer type VII for their proposed use as indirect pulp capping materials - Part II (A clinical study

    Directory of Open Access Journals (Sweden)

    N Sinha

    2011-01-01

    Full Text Available Aim: To evaluate in vivo the remineralizing efficacy of silver diamine fluoride (SDF, glass ionomer Type VII (GC VII and calcium hydroxide (Dycal. Materials and Methods: 60 subjects in the age group of 18-35 years, matching the inclusion criteria and having deep carious lesions in the permanent first and second molars were selected. The teeth were aseptically opened under rubber dam and after gross caries removal, approximately 0.4mg of soft discolored dentin was removed with a sharp spoon excavator from the mesial or distal aspect of the cavity. The test material was randomly selected and applied in a thickness of 1.5-2mm and the cavity sealed with cavit. The patients were followed up at regular intervals with radiographic evaluation at 12 weeks. At 3 months the temporary restoration was removed and dentin samples were collected from the other half of the cavity which was left in the first appointment. Atomic absorption spectrophotometry, Colorimetric test using UV-vis spectrometer and potentiometric titration were used for determining calcium, phosphorous and fluoride respectively. Results: Almost equivalent rise in the percentage of calcium level was seen in GC VII and Ca(OH 2 groups, followed by SDF group. Highest percentage rise in phosphate ions was seen in GC VII group followed by SDF group and Ca(OH 2 group. Highest percentage of fluoride rise was seen in GC VII group followed by SDF group and Ca(OH 2 group. Conclusions: The results indicated that both GC VII and SDF can be potential indirect pulp capping materials.

  10. Analyses of iron and concrete shielding experiments at JAEA/TIARA with JENDL/HE-2007, ENDF/B-VII.1 and FENDL-3.0

    Energy Technology Data Exchange (ETDEWEB)

    Konno, Chikara, E-mail: konno.chikara@jaea.go.jp; Ochiai, Kentaro; Sato, Satoshi; Ohta, Masayuki

    2015-10-15

    IAEA released a new Fusion Evaluated Nuclear Data Library, FENDL-3.0, in 2012. FENDL-3.0 extends the neutron energy range from 20 MeV to greater than 60 MeV. Now there is increasing interest in nuclear data above 20 MeV. Thus we have analyzed the iron and concrete shielding experiments with the 40 and 65 MeV neutron sources at TIARA in Japan Atomic Energy Agency with the latest high-energy nuclear data libraries, JENDL/HE-2007, ENDF/B-VII.1 and FENDL-3.0. The Monte Carlo code MCNP-5 and ACE files of JENDL/HE-2007, ENDF/B-VII.1 and FENDL-3.0, which are supplied from JAEA, LANL and IAEA, respectively, were used for this analysis. The collimated neutron beam and test shields were modeled in the analysis. The measured source neutron data were adopted in the analysis. The followings are found out from the results; (1) Iron experiments: The calculation result with FENDL-3.0 agrees with the measured one best. That with JENDL/HE-2007 fairly agrees with the measured one. On the contrary that with ENDF/B-VII.1 drastically overestimates the measured one. It is confirmed that this overestimation is due to the smaller non-elastic scattering data of {sup 56}Fe in ENDF/B-VII.1. (2) Concrete experiments: The calculation result with ENDL/HE-2007 agrees with the measured one best, while those with FENDL-3.0 and ENDF/B-VII.1 drastically overestimate the measured one. It is confirmed that this overestimation is due to both the larger elastic and smaller non-elastic scattering data of {sup 16}O in FENDL-3.0 and ENDF/B-VII.1.

  11. Bianchi type-I, -VII{sub 0} and -V cosmological models of Weyssenhoff fluid with magnetic moment

    Energy Technology Data Exchange (ETDEWEB)

    Lu, H. Q.; Shen, L. M.; Sun, N. J.; Ji, G. F. [Shangai Univ., Shangai (China). Dept. of Physics

    2001-07-01

    It is derived the system of field equations for a Weyssenhoff fluid including magnetic interaction among the spinning particles prevailing in spatially homogenous, but anisotropic cosmological models of Bianchi types I, VII{sub 0} and V based on the Einstein-Cartan theory. In this work are analyzed the field equations in three different equations of states specified by p=1/3 {rho}, p={rho} and p=0. It has obtained singular and non-singular solutions for Bianchi modes of types I and VII{sub 0} under the state p={rho} but have found that the non-singular solutions are unphysical. During the radiation epoch (p=1/3 {rho}) and the matter epoch (p=0), the analytical solutions found are non-singular for all the stated three Bianchi models, provided that the combined energy arising from matter spin and magnetic interaction among particles overcomes the anisotropy energy in the Universe. It has deduced that the minimum particle numbers for radiation and matter epochs are, respectively, 10{sup 88} and 10{sup 108}, leading to the conclusion that it must be considered the existence of neutrinos and other creation of particles and anti-particles under torsion and strong gravitational field in the early Universe.

  12. Evaluación de la asignatura Medicina General Integral VII en el policlínico universitario

    Directory of Open Access Journals (Sweden)

    Cynthia Olivia Morales Pérez

    2012-06-01

    Full Text Available Background: the subject General Medicine VII is part of the guiding discipline in Medicine studies. It ensures that when Medicine Doctors obtain their degrees they have acquired all the knowledge that allows them to perform multiple tasks in health care services. Objective: to assess the subject General Medicine VII, in the university polyclinic as part of the educational process. Methods: we conducted a mixed research using quantitative and qualitative methods through a methodology that included the level of satisfaction of students, teachers, managers, and knowledge levels obtained by students. This methodology was applied in the fourth year of Medicine studies and in the four university polyclinics in the municipality of Cienfuegos, inserted into the new training model. Results: managers referred insufficient chances for exchanging with subordinates as well as for the profits of methodological activities, teachers were dissatisfied because of insufficient educational preparation and high work load and students reported difficulties with the availability and on-time delivery of textbooks, in addition to not having the necessary instrumentation for skills appropriation. Conclusions: we found that managers were dissatisfied with organizational and functional aspects, teachers with planning and implementation of the teaching process and students with the literature and the necessary tools while in teaching practice.

  13. Identification of novel Newcastle disease virus sub-genotype VII-(j) based on the fusion protein.

    Science.gov (United States)

    Esmaelizad, Majid; Mayahi, Vafa; Pashaei, Maryam; Goudarzi, Hossein

    2017-04-01

    Newcastle disease virus (NDV) is believed to be the cause of fatal poultry disease worldwide. The fusion (F) protein plays a key role in virus pathogenesis, and it is also used for Newcastle disease virus classification. In this study, we determined the complete coding sequence of the F gene in new velogenic NDV isolates with an intravenous pathogenicity index (IVPI) of 1.8 and a mean death time (MDT) of 72 or 48 h. Complete sequences of the F genes of new Iranian isolates were amplified and sequenced in both directions. These isolates were compared to 195 nucleotide sequences from GenBank (available as of 07/17/2016). A phylogenetic tree was constructed for the F gene, using MEGA6 software with statistical analysis based on 500 bootstrap replicates. Evolutionary distances revealed that the new virulent isolates from Iran belonged to genotype VII in a new distinct sub-genotype named VII-(j). This new sub-genotype showed 3% divergence from genotype VIId. Recombination analysis showed that these new isolates were not recombinant NDVs.

  14. Soft X-ray emissions of highly charged Si VII--Si XII in cool star--Procyon

    CERN Document Server

    Liang, G Y

    2007-01-01

    Different observation data for cool star--Procyon (Obs\\_IDs of 63, 1461 and 1224) available from {\\it Chandra Data Public Archive} were co-added and analyzed. Emissivities of emission lines of highly charged silicon ions (Si VII--Si XII) were calculated over temperatures by adopting the published data of Liang et al. (2007, {\\it Atom. Data and Nucl. Data Tables}, {\\bf 93}, 375). Using the emission measure derived by Raassen et al. (2002, A&A, {\\bf 389}, 228), the theoretical line fluxes are predicted, and the theoretical spectra are constructed by assuming the Gaussian profile with instrumental broadening (0.06 \\AA). By detailed comparison between observation and predictions, several emissions lines are identified firstly such as emissions at 43.663 \\AA (Si XI), 45.550 \\AA (Si XII), 46.179 \\AA (Si VIII), 50.874 \\AA (Si X), 64.668 \\AA (Si IX), and 73.189 \\AA (Si VII) etc. Several emission lines are re-assigned in this work, such as the emission line at 52.594 \\AA to Si X (52.612 \\AA), at 69.641 \\AA to the ...

  15. Changes of androgen receptor expression in stages VII-VIII seminiferous tubules of rat testis after exposure to methamphetamine

    Directory of Open Access Journals (Sweden)

    Sutita Nudmamud-Thanoi

    2016-06-01

    Full Text Available Methamphetamine (METH is toxic not only to the central nervous system but also to the reproductive system. This study aimed to investigate the effect of METH administration on androgen receptor (AR expression in the seminiferous tubules (stages VII-VIII and Leydig cells. 24 Sprague-Dawley rats were equally divided into 4 groups; control, acute doseMETH binge (AB-METH, escalating dose-METH (ED-METH and escalating dose-METH binge (ED-METH binge groups. The expressions of AR were examined using immunohistochemical staining. AR expressions in round and elongated spermatids showed significant decreases in ED-METH binge and AB-METH groups. In Leydig’s cells, AR expressions were significantly decreased in both ED-METH and ED-METH binge groups. These results could reflect the effect of METH on the expressions of AR in both round and elongated spermatids of stages VII-VIII seminiferous tubule and Leydig’s cells. Decreases of AR expressions in those cells of METH-treated animals may interrupt spermatogenesis at these stages.

  16. A broad-group cross-section library based on ENDF/B-VII.0 for fast neutron dosimetry Applications

    Energy Technology Data Exchange (ETDEWEB)

    Alpan, F.A. [Westinghouse Electric Company, 1000 Westinghouse Drive, Cranberry Township, PA 16066 (United States)

    2011-07-01

    A new ENDF/B-VII.0-based coupled 44-neutron, 20-gamma-ray-group cross-section library was developed to investigate the latest evaluated nuclear data file (ENDF) ,in comparison to ENDF/B-VI.3 used in BUGLE-96, as well as to generate an objective-specific library. The objectives selected for this work consisted of dosimetry calculations for in-vessel and ex-vessel reactor locations, iron atom displacement calculations for reactor internals and pressure vessel, and {sup 58}Ni(n,{gamma}) calculation that is important for gas generation in the baffle plate. The new library was generated based on the contribution and point-wise cross-section-driven (CPXSD) methodology and was applied to one of the most widely used benchmarks, the Oak Ridge National Laboratory Pool Critical Assembly benchmark problem. In addition to the new library, BUGLE-96 and an ENDF/B-VII.0-based coupled 47-neutron, 20-gamma-ray-group cross-section library was generated and used with both SNLRML and IRDF dosimetry cross sections to compute reaction rates. All reaction rates computed by the multigroup libraries are within {+-} 20 % of measurement data and meet the U. S. Nuclear Regulatory Commission acceptance criterion for reactor vessel neutron exposure evaluations specified in Regulatory Guide 1.190. (authors)

  17. EFEKTIVITAS PERSYARATAN HYGIENE SANITASI TERHADAP USAHA RUMAH MAKAN DAN RESTORAN BERDASARKAN KEPMENKES RI NOMOR 1098/MENKES/SK/VII/2003

    Directory of Open Access Journals (Sweden)

    Gusti Ayu Dita Nomia Sari

    2016-09-01

    Full Text Available Tourists who visited tourism destination in addition to the cultural and natural environment, food and drinks being the main attraction. Lack of awareness of the restaurant business to maintain cleanliness and sanitation are not uncommon cause of food poisoning. Legal issues raised in this study are: a How obligation of entrepreneur restaurants and legal protection for consumers under the Consumer Protection Act; b how the effectiveness of sanitation hygiene is based on Kepmenkes No. 1098 / Menkes / SK / VII / 2003 against the restaurants in the Ubud area. This study uses empirical legal research . The data is processed by analyzing qualitative descriptive analytical. Wisatawan yang mengunjungi destinasi pariwisata selain budaya dan keindahan alam, makanan dan minuman menjadi daya tarik utama lainnya. Kurangnya kesadaran pengusaha restoran untuk menjaga kebersihan dan sanitasi yang tidak jarang menyebabkan terjadinya keracunan makanan. Isu hukum yang diangkat dalam penelitian ini adalah: a Bagaimana kewajiban pelaku usaha rumah makan dan restoran dan perlindungan hukum bagi konsumen menurut UU Perlindungan Konsumen; b bagaimana efektivitas hygiene sanitasi restoran berdasarkan Kepmenkes No. 1098 / Menkes / SK / VII / 2003 terhadap rumah makan dan restoran di kawasan ubud. Penelitian ini menggunakan penelitian hukum empiris . Data diolah dengan menganalisis deskriptif kualitatif analitis.

  18. Characterization of Staphylococcus aureus EssB, an integral membrane component of the Type VII secretion system: atomic resolution crystal structure of the cytoplasmic segment

    OpenAIRE

    Zoltner, Martin; Fyfe, Paul K.; Palmer, Tracy; Hunter, William N.

    2012-01-01

    The Type VII protein translocation/secretion system, unique to Gram-positive bacteria, is a key virulence determinant in Staphylococcus aureus. We aim to characterize the architecture of this secretion machinery and now describe the present study of S. aureus EssB, a 52 kDa bitopic membrane protein essential for secretion of the ESAT-6 (early secretory antigenic target of 6 kDa) family of proteins, the prototypic substrate of Type VII secretion. Full-length EssB was heterologously expressed i...

  19. Comportamiento clínico epidemiológico del absceso dentoalveolar agudo en pacientes pertenecientes al área VII de Cienfuegos

    OpenAIRE

    Ana Belkys Hernández Millán; Zenia Lizette Hernández Millán; Regla Martínez de la Cotera Molina; Mercedes Verónica Diego Cobelo; Diosky Ferrer Vilches; Nora Sexto Delgado

    2015-01-01

    Fundamento: el absceso dentoalveolar agudo es una de las urgencias estomatológicas y una de las principales enfermedades que afectan a la población, sin embargo existen muy pocos estudios sobre el tema.Objetivo: describir comportamiento clínico epidemiológico del absceso dentoalveolar agudo en pacientes pertenecientes al área VII, de Cienfuegos. Métodos: investigación observacional, descriptiva realizada de enero a diciembre de 2013 en el Área VII, de Cienfuegos. El universo fue de 672 pacien...

  20. Estimation of the Dose and Dose Rate Effectiveness Factor

    Science.gov (United States)

    Chappell, L.; Cucinotta, F. A.

    2013-01-01

    Current models to estimate radiation risk use the Life Span Study (LSS) cohort that received high doses and high dose rates of radiation. Transferring risks from these high dose rates to the low doses and dose rates received by astronauts in space is a source of uncertainty in our risk calculations. The solid cancer models recommended by BEIR VII [1], UNSCEAR [2], and Preston et al [3] is fitted adequately by a linear dose response model, which implies that low doses and dose rates would be estimated the same as high doses and dose rates. However animal and cell experiments imply there should be curvature in the dose response curve for tumor induction. Furthermore animal experiments that directly compare acute to chronic exposures show lower increases in tumor induction than acute exposures. A dose and dose rate effectiveness factor (DDREF) has been estimated and applied to transfer risks from the high doses and dose rates of the LSS cohort to low doses and dose rates such as from missions in space. The BEIR VII committee [1] combined DDREF estimates using the LSS cohort and animal experiments using Bayesian methods for their recommendation for a DDREF value of 1.5 with uncertainty. We reexamined the animal data considered by BEIR VII and included more animal data and human chromosome aberration data to improve the estimate for DDREF. Several experiments chosen by BEIR VII were deemed inappropriate for application to human risk models of solid cancer risk. Animal tumor experiments performed by Ullrich et al [4], Alpen et al [5], and Grahn et al [6] were analyzed to estimate the DDREF. Human chromosome aberration experiments performed on a sample of astronauts within NASA were also available to estimate the DDREF. The LSS cohort results reported by BEIR VII were combined with the new radiobiology results using Bayesian methods.

  1. Angioma cavernoso do meato acústico interno envolvendo o complexo VII e VIII nervos cranianos: relato de caso Cavernous hemangioma of the internal auditory canal encasing the VII and VIII cranial nerve complex: case report

    Directory of Open Access Journals (Sweden)

    Roberto Leal Silveira

    2005-03-01

    Full Text Available Relata-se o caso de homem de 21 anos, leucoderma, portador de angioma cavernoso do meato acústico interno direito envolvendo o complexo VII e VIII nervos cranianos tratado cirurgicamente. Apenas 18 casos de angiomas cavernosos dessa localização foram relatados na literatura. São comentados os aspectos clínicos, o diagnóstico diferencial e o tratamento.We report the surgically treated case of a 21-year-old caucasian male harboring a cavernous hemangioma of the right internal auditory canal encasing the seventh and eighth cranial nerves complex. Only 18 cases of cavernous hemangiomas of this location have been previously reported. The clinical features, the differential diagnosis and the treatment are discussed.

  2. CONSTRAINING THE MILKY WAY'S HOT GAS HALO WITH O VII AND O VIII EMISSION LINES

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Matthew J.; Bregman, Joel N., E-mail: mjmil@umich.edu, E-mail: jbregman@umich.edu [Department of Astronomy, University of Michigan, Ann Arbor, MI 48104 (United States)

    2015-02-10

    The Milky Way hosts a hot (≈2 × 10{sup 6} K), diffuse, gaseous halo based on detections of z = 0 O VII and O VIII absorption lines in quasar spectra and emission lines in blank-sky spectra. Here we improve constraints on the structure of the hot gas halo by fitting a radial model to a much larger sample of O VII and O VIII emission line measurements from XMM-Newton/EPIC-MOS spectra compared to previous studies (≈650 sightlines). We assume a modified β-model for the halo density distribution and a constant-density Local Bubble from which we calculate emission to compare with the observations. We find an acceptable fit to the O VIII emission line observations with χ{sub red}{sup 2} (dof) = 1.08 (644) for best-fit parameters of n{sub o}r{sub c}{sup 3β}=1.35±0.24 cm{sup –3} kpc{sup 3β} and β = 0.50 ± 0.03 for the hot gas halo and negligible Local Bubble contribution. The O VII observations yield an unacceptable χ{sub red}{sup 2} (dof) = 4.69 (645) for similar best-fit parameters, which is likely due to temperature or density variations in the Local Bubble. The O VIII fitting results imply hot gas masses of M(<50 kpc) = 3.8{sub −0.3}{sup +0.3}×10{sup 9} M{sub ⊙} and M(<250 kpc) = 4.3{sub −0.8}{sup +0.9}×10{sup 10} M{sub ⊙}, accounting for ≲50% of the Milky Way's missing baryons. We also explore our results in the context of optical depth effects in the halo gas, the halo gas cooling properties, temperature and entropy gradients in the halo gas, and the gas metallicity distribution. The combination of absorption and emission line analyses implies a sub-solar gas metallicity that decreases with radius, but that also must be ≥0.3 Z {sub ☉} to be consistent with the pulsar dispersion measure toward the Large Magellanic Cloud.

  3. Uudised : Don Juan jõuab Estonia lavale. VII Rudolf Tobiase päevad Hiiumaal. Esineb Laine Leichteri koolkond / Liina Teder

    Index Scriptorium Estoniae

    Teder, Liina

    1999-01-01

    3.juunil Estonias esietenduvast Mozarti ooperist "Don Giovanni". 28.-29.05 toimuvatest VII R. Tobiase päevadest, mis on pühendatud helilooja 126. sünniaastapäevale. 3.06 Mustpeade Majas toimuvast L. Leichteri juubelikontserdist

  4. Retsensioon : Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare. Peatoimetaja Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. 326 lk. / Mart Nutt

    Index Scriptorium Estoniae

    Nutt, Mart, 1962-

    2004-01-01

    Arvustus: Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare / peatoim. Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. - Lisatud: Ott Heinapuu tutvustav sõnum retsenseeritud väljaande kohta lk. 193. - Vt. ka: http://www.riigikogu.ee/rito/index.php?id=11357

  5. Are Churches above the Law? The Application of the Fair Labor Standards Act and the Equal Pay Provisions of Title VII to Religious Organizations.

    Science.gov (United States)

    Beardsley, Alberta

    1979-01-01

    Shows that application of the Fair Labor Standards Act and Title VII to religious organizations and lay employees does not violate the free exercise clause, whereas a blanket exemption might run afoul of the establishment clause. Available from University of Pittsburgh Law Review, 3900 Forbes Avenue, Pittsburgh, PA 15260; single copies $2.50.…

  6. PENINGKATAN AKTIVITAS DAN HASIL BELAJAR MENGGUNAKAN METODE EKSPERIMEN PADA SISWA KELAS VII SMP NEGERI 1 PURBOLINGGO TAHUN PELAJARAN 2013/2014

    Directory of Open Access Journals (Sweden)

    Endang Kurniawati

    2014-03-01

    Full Text Available The purposeofthis studyis: to describethe use ofexperimental method sin enhancingthe activity oflearning physicsclass VII students of SMPN1Purbolinggoandto describethe use ofexperimentalmethodsin improvingstudentlearning outcomesphysicsclass VII SMP N 1 Purbolinggo.This study uses a Class Action Research and 4-cycle stages: planning, action, observation and reflection for further action. The study lasted 2 cycles each cycle consisting of 2 meetings. As the subjects in this study were VII.F grade students of SMP N 1 Purbolinggo of 40 people. Means of collecting data in the form of sheets of observations and tests. From the analysis and the results of this study concluded that the use of experimental methods in the learning process can improve the activity and student learning outcomes VII.F SMP N 1 Purbolinggo on the material and temperature measurements with an average of 11.56% increase in activity. Average learning outcomes increased by 55%. Thus it can be advisable for teachers to improve the activity and student learning outcomes using experimental methods.

  7. Integrating Title I and Title VII: The Evolving Model of Dearborn Public Schools, Michigan. NCBE Program Information Guide Series No. 21.

    Science.gov (United States)

    Arraf, Shereen; And Others

    This report offers insights on the experiences and techniques used in the Dearborn Public Schools effort to integrate Title VII and Chapter 1 programs prior to their reauthorization. The school system is located near metropolitan Detroit, Michigan, and has over 14,500 students representing 24 languages, many from the Middle East, Romania, and…

  8. 29 CFR 1605.2 - Reasonable accommodation without undue hardship as required by section 701(j) of title VII of the...

    Science.gov (United States)

    2010-07-01

    ... BECAUSE OF RELIGION § 1605.2 Reasonable accommodation without undue hardship as required by section 701(j... address other obligations under title VII not to discriminate on grounds of religion, nor other provisions... intended to provide guidance for statutes which require accommodation on bases other than religion such...

  9. Dos Idiomas, Un Mundo Dual Language Project Title VII Third-Year Evaluation Report, 1997-98. Publication Number 97.21.

    Science.gov (United States)

    Liberty, Paul; Gonzalez, Rosa Maria

    An evaluation was conducted of the third year of a 5-year comprehensive bilingual education grant funded by Title VII of the Improving America's Schools Act of 1994. The grant funds a program of developmental bilingual education at two elementary schools in the Austin Independent School District (Texas). More than 25% of the students at each…

  10. Sex Discrimination and Hair-Length Requirements Under Title VII of the Civil Rights Act of 1964 -- the Long and Short of It

    Science.gov (United States)

    Golden, H. Bruce

    1974-01-01

    Since the passage of Title VII of the Civil Rights Act of 1964, an employer's rights to establish dress and appearance standards for employees has been challenged with increasing frequency as a discriminatory employment practice based upon sex. The majority of such cases have concerned the right of male employees to wear their hair as long as that…

  11. Caminos Bilingues al Exito=Bilingual Pathways to Success: A Title VII Bilingual Education Program 1995-96. Research Report on Educational Grants.

    Science.gov (United States)

    Houston Independent School District, TX. Dept. of Research and Evaluation.

    Caminos Bilingues al Exito (Bilingual Pathways to Success), a comprehensive Title VII Bilingual Education program was established in 1995 to serve the 17 schools of the North Area Management District of the Houston Independent School District (Texas). Through teacher training, staff development, parental involvement, and instructional program…

  12. Caminos Bilingues al Exito=Bilingual Pathways to Success: A Title VII Bilingual Education Program 1995-96. Research Report on Educational Grants.

    Science.gov (United States)

    Houston Independent School District, TX. Dept. of Research and Evaluation.

    Caminos Bilingues al Exito (Bilingual Pathways to Success), a comprehensive Title VII Bilingual Education program was established in 1995 to serve the 17 schools of the North Area Management District of the Houston Independent School District (Texas). Through teacher training, staff development, parental involvement, and instructional program…

  13. X Congreso Argentino de Paleontología y Bioestratigrafía y VII Congreso Latinoamericano de Paleontología : Resúmenes

    OpenAIRE

    Ballent, Sara; Analía E. Artabe; Tortello, M. Franco

    2010-01-01

    Resúmenes de los trabajos presentados en el X Congreso Argentino de Paleontología y Bioestratigrafía y VII Congreso Latinoamericano de Paleontología realizado en la ciudad de La Plata los días 20 al 24 de septiembre de 2010

  14. Retsensioon : Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare. Peatoimetaja Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. 326 lk. / Mart Nutt

    Index Scriptorium Estoniae

    Nutt, Mart, 1962-

    2004-01-01

    Arvustus: Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare / peatoim. Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. - Lisatud: Ott Heinapuu tutvustav sõnum retsenseeritud väljaande kohta lk. 193. - Vt. ka: http://www.riigikogu.ee/rito/index.php?id=11357

  15. Hypoperfusion in severely injured trauma patients is associated with reduced coagulation factor activity.

    Science.gov (United States)

    Jansen, Jan O; Scarpelini, Sandro; Pinto, Ruxandra; Tien, Homer C; Callum, Jeannie; Rizoli, Sandro B

    2011-11-01

    Recent studies have shown that acute traumatic coagulopathy is associated with hypoperfusion, increased plasma levels of soluble thrombomodulin, and decreased levels of protein C but with no change in factor VII activity. These findings led to the hypothesis that acute traumatic coagulopathy is primarily due to systemic anticoagulation, by activated protein C, rather than decreases in serine protease activity. This study was designed to examine the effect of hypoperfusion secondary to traumatic injury on the activity of coagulation factors. Post hoc analysis of prospectively collected data on severely injured adult trauma patients presenting to a single trauma center within 120 minutes of injury. Venous blood was analyzed for activity of factors II, V, VII, VIII, IX, X, and XI. Base deficit from arterial blood samples was used as a marker of hypoperfusion. Seventy-one patients were identified. The activity of factors II, V, VII, IX, X, and XI correlated negatively with base deficit, and after stratification into three groups, based on the severity of hypoperfusion, a statistically significant dose-related reduction in the activity of factors II, VII, IX, X, and XI was observed. Hypoperfusion is also associated with marked reductions in factor V activity levels, but these appear to be relatively independent of the degree of hypoperfusion. The activity of factor VIII did not correlate with base deficit. Hypoperfusion in trauma patients is associated with a moderate, dose-dependent reduction in the activity of coagulation factors II, VII, IX, X, and XI, and a more marked reduction in factor V activity, which is relatively independent of the severity of shock. These findings suggest that the mechanisms underlying decreased factor V activity--which could be due to activated protein C mediated cleavage, thus providing a possible link between the proposed thrombomodulin/thrombin-APC pathway and the serine proteases of the coagulation cascade--and the reductions in factors

  16. KEEFEKTIFAN PEMBELAJARAN KETERAMPILAN MENULIS CERPEN DENGAN MODEL INVESTIGASI KELOMPOK DAN MODEL STAD BERDASARKAN TIPE KEPRIBADIAN PESERTA DIDIK KELAS VII

    Directory of Open Access Journals (Sweden)

    Agus Pujianto

    2015-03-01

    Full Text Available Tujuan penelitian ini adalah menguji keefektifan pembelajaran keterampilan menulis cerpen dengan model investigasi kelompok dan model STAD berdasarkan tipe kepribadian peserta didik kelas VII. Keterampilan menulis cerpen membutuhkan kreativitas dalam pelaksanaan pembelajaran. Melalui keterampilan menulis cerpen siswa dapat berkreasi untuk mengemukakan ide atau gagasan yang ingin disampaikan kepada pembaca. Ide atau gagasan juga berpengaruh terhadap hasil dalam menulis cerpen. Selain itu, pengetahuan tentang cerpen juga penting dalam pembelajaran keterampilan menulis cerpen. Siswa perlu berpikir kritis untuk memunculkan ide atau gagasan yang menarik dan untuk pemahaman mengenai unsur-unsur cerpen. Tipe kepribadian yang berbeda juga perlu membutuhkan cara pengajaran yang berbeda. Oleh karena itu, dibutuhkan model pembelajaran yang cocok. Model pembelajaran yang dipilih adalah model investigasi kelompok dan model STAD.

  17. Abstracts of contributions presented in the VII. International Conference on the Conservation of the Eastern Imperial Eagle, Bratislava, Slovakia, 2013

    Directory of Open Access Journals (Sweden)

    Deutschová Lucia

    2014-01-01

    Full Text Available VII. International Conference on the Conservation of the Eastern Imperial Eagle (Aquila heliaca was held on October 2-5, 201 3 in Barónka hotel in Bratislava, Slovakia and it was organised by Raptor Protection of Slovakia (RPS in cooperation with the Czech Society for Ornithology and MME/BirdLife Hungary, under the auspices of the Ministry of Environment, supported by the International Visegrad Fund. Results of 43 experts from ten countries, including three countries of Visegrad region (Slovakia, Czech Republic and Hungary were presented in 20 presentations and five posters. Six contributions are published in Slovak Raptor Journal volume 8, issue 1 (2014 as full papers, further twelve contributions are published here as conference abstracts.

  18. Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus.

    Science.gov (United States)

    Jäger, Franziska; Zoltner, Martin; Kneuper, Holger; Hunter, William N; Palmer, Tracy

    2016-02-01

    The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.

  19. Motor palsies of cranial nerves (excluding VII) after vaccination: reports to the US Vaccine Adverse Event Reporting System.

    Science.gov (United States)

    Woo, Emily Jane; Winiecki, Scott K; Ou, Alan C

    2014-01-01

    We reviewed cranial nerve palsies, other than VII, that have been reported to the US Vaccine Adverse Event Reporting System (VAERS). We examined patterns for differences in vaccine types, seriousness, age, and clinical characteristics. We identified 68 reports of cranial nerve palsies, most commonly involving the oculomotor (III), trochlear (IV), and abducens (VI) nerves. Isolated cranial nerve palsies, as well as palsies occurring as part of a broader clinical entity, were reported. Forty reports (59%) were classified as serious, suggesting that a cranial nerve palsy may sometimes be the harbinger of a broader and more ominous clinical entity, such as a stroke or encephalomyelitis. There was no conspicuous clustering of live vs. inactivated vaccines. The patient age range spanned the spectrum from infants to the elderly. Independent data may help to clarify whether, when, and to what extent the rates of cranial nerve palsies following particular vaccines may exceed background levels.

  20. Calculation of Maxwellian-averaged cross sections and their uncertainties using ENDF/B-VII.1 evaluated neutron library

    Science.gov (United States)

    Pritychenko, Boris

    2011-10-01

    Present contribution represents a first application of ENDF/B-VII.1 neutron library for calculation of Maxwellian-averaged cross sections and astrophysical reaction rates. Recent improvements in neutron cross section evaluations and more extensive utilization of covariance files, by the CSEWG collaboration, allowed us to perform complete calculations and provide additional insights on all currently available neutron-induced reaction data. Nuclear reaction calculations using ENDF libraries and current Java technologies will be discussed and new results will be presented. This work was sponsored by the Office of Nuclear Physics, Office of Science of the U.S. Department of Energy, under Contract No. DE-AC02-98CH10886 with Brookhaven Science Associates, LLC.

  1. Complete plasmid sequence carrying type IV-like and type VII secretion systems from an atypical mycobacteria strain.

    Science.gov (United States)

    Morgado, Sergio Mascarenhas; Marín, Michel Abanto; Freitas, Fernanda S; Fonseca, Erica Lourenço; Vicente, Ana Carolina Paulo

    2017-07-01

    The genus Mycobacterium is highly diverse and ubiquitous in nature, comprehending fast- and slow-growing species with distinct impact in public health. The plasmid-mediated horizontal gene transfer represents one of the major events in bacteria evolution. Here, we report the complete sequence of a 160,489 bp circular plasmid (pCBMA213_2) from an atypical and fast-growing environmental mycobacteria. This is a unique plasmid, in comparison with the characterised mycobacteria plasmids, harboring a type IV-like and ESX-P2 type VII secretion systems. pCBMA213_2 can be further explored for evolutionary and conjugation studies as well as a tool to manipulate DNA within this bacteria genus.

  2. Lealtad y fidelidad: la proclamación del rey Fernando VII en Cartago, provincia de Costa Rica, 1809

    Directory of Open Access Journals (Sweden)

    Guillermo Brenes Tencio

    2009-01-01

    Full Text Available Durante el período colonial, las fiestas reales convierten a las ciudades hispanoamericanas en el escenario público en que se representa el fascinante espectáculo del imaginario monárquico. Todas las colonias del Imperio español en América proclamaban a un monarca físicamente ausente, pero materializado simbólicamente a través de representaciones discursivas e iconográficas. En este escrito se describen y analizan los aspectos más interesantes de la proclamación o ceremonia de jura del rey Fernando VII, en la ciudad colonial de Cartago, entre los días 15 y 23 de enero de 1809.

  3. Assessment of bone dysplasia by micro-CT and glycosaminoglycan levels in mouse models for mucopolysaccharidosis type I, IIIA, IVA, and VII

    Science.gov (United States)

    Rowan, Daniel J.; Tomatsu, Shunji; Grubb, Jeffrey H.; Montaño, Adriana M.; Sly, William S.

    2012-01-01

    Summary Mucopolysaccharidoses (MPS) are a group of lysosomal storage diseases caused by mutations in lysosomal enzymes involved in degradation of glycosaminoglycans (GAGs). Patients with MPS grow poorly and become physically disabled due to systemic bone disease. While many of the major skeletal effects in mouse models for MPS have been described, no detailed analysis that compares GAGs levels and characteristics of bone by micro-CT has been done. The aims of this study were to assess severity of bone dysplasia among four MPS mouse models (MPS I, IIIA, IVA and VII), to determine the relationship between severity of bone dysplasia and serum keratan sulfate (KS) and heparan sulfate (HS) levels in those models, and to explore the mechanism of KS elevation in MPS I, IIIA, and VII mouse models. Clinically, MPS VII mice had the most severe bone pathology; however, MPS I and IVA mice also showed skeletal pathology. MPS I and VII mice showed severe bone dysplasia, higher bone mineral density, narrowed spinal canal, and shorter sclerotic bones by micro-CT and radiographs. Serum KS and HS levels were elevated in MPS I, IIIA, and VII mice. Severity of skeletal disease displayed by micro-CT, radiographs and histopathology correlated with the level of KS elevation. We showed that elevated HS levels in MPS mouse models could inhibit N-acetylgalactosamine-6-sulfate sulfatase enzyme. These studies suggest that KS could be released from chondrocytes affected by accumulation of other GAGs and that KS could be useful as a biomarker for severity of bone dysplasia in MPS disorders. PMID:22971960

  4. Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus.

    Science.gov (United States)

    Rasoli, Mehdi; Yeap, Swee Keong; Tan, Sheau Wei; Moeini, Hassan; Ideris, Aini; Bejo, Mohd Hair; Alitheen, Noorjahan Banu Mohamed; Kaiser, Pete; Omar, Abdul Rahman

    2014-01-01

    Newcastle disease (ND) is a highly contagious avian disease and one of the major causes of economic losses in the poultry industry. The emergence of virulent NDV genotypes and repeated outbreaks of NDV in vaccinated chickens have raised the need for fundamental studies on the virus-host interactions. In this study, the profiles of B and T lymphocytes and macrophages and differential expression of 26 immune-related genes in the spleen of specific-pathogen-free (SPF) chickens, infected with either the velogenic genotype VII NDV strain IBS002 or the genotype VIII NDV strain AF2240, were evaluated. A significant reduction in T lymphocyte population and an increase in the infiltration of IgM+ B cells and KUL01+ macrophages were detected in the infected spleens at 1, 3 and 4 days post-infection (dpi) (P<0.05). The gene expression profiles showed an up-regulation of CCLi3, CXCLi1, CXCLi2 (IL-8), IFN-γ, IL-12α, IL-18, IL-1β, IL-6, iNOS, TLR7, MHCI, IL-17F and TNFSF13B (P<0.05). However, these two genotypes showed different cytokine expression patterns and viral load. IBS002 showed higher viral load than AF2240 in spleen at 3 and 4dpi and caused a more rapid up-regulation of CXCLi2, IFN-γ, IL-12α, IL-18, IL-1β, iNOS and IL-10 at 3dpi. Meanwhile, the expression levels of CCLI3, CXCLi1, IFN-γ, IL-12α, IL-1β and iNOS genes were significantly higher in AF2240 at 4dpi. In addition, the expression levels of IL-10 were significantly higher in the IBS002-infected chickens at 3 and 4dpi. Hence, infection with velogenic genotype VII and VIII NDV induced different viral load and production of cytokines and chemokines associated with inflammatory reactions.

  5. Un protector laminado de brazo (manica procedente del campamento de la legio VII Gemina en León

    Directory of Open Access Journals (Sweden)

    Aurrecoechea, Joaquín

    2008-12-01

    Full Text Available At the archaeological excavations carried out during 1998 in Santa Marina street, near the internal face of the Late Roman wall of Leon, an important building of the legio VII gemina fortress was discovered. It was identified as a magazine with a central courtyard, erected by this legionary unit at the end of the 1st c. AD. Inside one of the constructions a fragment of a manica or a laminated arm protector was found. This element consists of 11 overlapping plates of different sizes, four of them trapezoid and seven rectangular and of greater size. Some fragments were preserved, which could have been leather. This ensemble became deformed by crushing. This protector was abandoned around the last quarter of the 3rd c. AD in a room which was possibly a storage area and was crushed by the collapse of the roof of the building.Las excavaciones arqueológicas desarrolladas durante 1998 en un solar de la calle Santa Marina, inmediato a la cara interna de la muralla bajoimperial de León, revelaron un gran edificio perteneciente al campamento de la legio VII gemina, que ha sido identificado como un almacén con patio central, levantado a finales del siglo I d. C. En el interior de una de las estancias se encontró un fragmento de manica o protector laminado de brazo. Este elemento está constituido por 11 láminas imbricadas de distinto tamaño, cuatro trapezoidales y siete rectangulares de mayor tamaño. Se conservan algunos restos de lo que pudiera ser cuero. Este conjunto se encuentra deformado por aplastamiento. El protector fue abandonado hacia el último cuarto del siglo III d. C. en una estancia destinada posiblemente a almacenamiento y quedó aplastado por el derrumbe del techo del edificio.

  6. Scale Factor Study for 1:30 Local Scour Model

    Science.gov (United States)

    2016-08-01

    Sedimentation engineering : ASCE manuals and reports on engineering practice, No. 54. Reston, VA: American Society of Civil Engineers ...Jeremy A. Sharp, Ronald E. Heath, Howard E. Park, and Tate O. McAlpin PURPOSE: This Coastal and Hydraulics Engineering Technical Note (CHETN... Engineer Research and Development Center, Vicksburg, MS. ERDC/CHL CHETN-VII-15 August 2016 8 For implementation, the scale factor was applied to the

  7. Relato do primeiro paciente brasileiro com a forma infantil da doença de Pompe tratado com alfa-glicosidase recombinante humana Report of the first Brazilian infantile Pompe disease patient to be treated with recombinant human acid alpha-glucosidase

    Directory of Open Access Journals (Sweden)

    Sandra J. Pereira

    2008-06-01

    Full Text Available Objetivo: Relatar o primeiro caso de forma infantil da doença de Pompe tratado no Brasil. Descrição: Trata-se de doença de depósito lisossomal que se caracteriza por defeitos da enzima alfa-glicosidase ácida, com acúmulo intracelular de glicogênio, principalmente nos músculos. São descritas a forma infantil e tardia. Desde 2006, está disponível tratamento com enzima recombinante humana. Descreve-se o primeiro caso de forma infantil da doença tratado no Brasil. Trata-se de menina com 2,5 meses de idade e progressão rápida da doença, com perda dos movimentos dos membros, miocardiopatia hipertrófica e insuficiência respiratória aos 7 meses de idade. Após 10 meses de tratamento, apresentou boa resposta clínica, com remissão da insuficiência respiratória, recuperação parcial dos movimentos dos membros e melhora importante do quadro cardiológico. Comentários: Apesar de pouco freqüente, a forma infantil da doença de Pompe é letal. A disponibilidade de tratamento eficaz aumenta a necessidade de conhecimento e diagnóstico precoce da doença.Objective: To describe the first case of infantile Pompe disease to be treated in Brazil. Description: Pompe disease is a glycogen storage disease related to defects in the acid alpha-glucosidase enzyme, leading to an intracellular accumulation of glycogen, mainly in muscles. Two forms are described: infantile and juvenile. Since 2006, treatment with recombinant human acid alpha-glucosidase has been available. This article describes the first case of infantile Pompe disease treated in Brazil. A girl presented at 2.5 months of age with rapid disease progression, exhibiting severe hypotonia, loss of movements in both upper and lower limbs and hypertrophic cardiomyopathy, progressing to respiratory failure by the age of 7 months. After 10 months of treatment, she exhibited a good clinical response, with remission of the respiratory failure, partial recovery of arm and leg movements and

  8. Efeito da utilização da somatotropina bovina recombinante (bST sobre a produção de leite em búfalas Effect of recombinant bovine somatotropin (bST utilization on milk production from buffaloes

    Directory of Open Access Journals (Sweden)

    André Mendes Jorge

    2002-06-01

    Full Text Available O objetivo deste trabalho foi estudar o resultado da utilização da somatotropina bovina recombinante (bST atuando na produção de leite em búfalas da raça Murrah. Empregaram-se 28 búfalas multíparas da raça Murrah, divididas em dois grupos homogêneos de 14 animais, recebendo os seguintes tratamentos: Grupo 1 ¾ Controle (solução salina e Grupo 2 ¾ 500 mg de bST/cabeça a cada 14 dias, durante 7 meses. Búfalas tratadas com bST exibiram incrementos de 48,52%, 32,80% e 32,80% nas produções total de leite, corrigida, depois, para 4% de gordura e média diária, respectivamente. A somatotropina elevou a produção total de gordura sem alterar a porcentagem dela no leite. A administração de bST não afetou a porcentagem de proteína do leite todavia, a produção total de proteína foi aumentada. Quanto à duração da lactação, o tratamento com bST diferiu do controle, o que demonstra a maior persistência da lactação de búfalas tratadas com bST.The objective of this work was to study the effect of recombinant bovine somatotropin (bST utilization on milk production from Murrah buffaloes. Twenty eight multiparous Murrah buffaloes were used and divided into two homogeneous groups of 14 animals, receiving the following treatments: Group 1 ¾ Control (salt solution and Group 2 ¾ 500 mg of bST/head every 14 days during 7 months. Buffaloes treated with bST presented increase of 48.52%, 32.80% and 32.80% on total milk yield, adjusted to 4% of fat and average daily milk yield, respectively. Somatotropin increased total fat milk yield without alter fat percentage of milk. Administration of bST did not affect protein percentage of milk while total protein milk yield increased. As for the lactating period, the treatment with bST differed of the control, what might have denoted in larger persistence of the lactation from buffaloes treated with bST.

  9. Pesquisa de IgA contra o antígeno recombinante HspX de Mycobacterium tuberculosis no diagnóstico de tuberculose pleural Determination of levels of specific IgA to the HspX recombinant antigen of Mycobacterium tuberculosis for the diagnosis of pleural tuberculosis

    Directory of Open Access Journals (Sweden)

    Loanda Carvalho Sant' Ana Limongi

    2011-06-01

    Full Text Available OBJETIVO: Avaliar a acurácia da dosagem de IgA contra o antígeno recombinante HspX no líquido pleural e no soro de pacientes com derrame pleural para o diagnóstico de tuberculose pleural. MÉTODOS: Estudo transversal de teste diagnóstico. Amostras de líquido pleural e de soro de pacientes com derrame pleural e suspeita de tuberculose pleural foram avaliadas para a determinação da densidade óptica de IgA contra HspX utilizando ELISA indireto. RESULTADOS: Foram avaliadas amostras de líquido pleural e de soro de 132 pacientes: 97 com tuberculose pleural (grupo de estudo e 35 com derrame pleural por outras causas (grupo controle. A dosagem de IgA em líquido pleural foi capaz de discriminar os pacientes com tuberculose pleural dos controles. A sensibilidade do teste em líquido pleural e em soro foi, respectivamente, de 69% e 30%, enquanto a especificidade foi de 83% e 84%, respectivamente. CONCLUSÕES: Os dados sugerem o potencial da utilização deste teste no diagnóstico de tuberculose pleural. Estudos com amostras maiores e em diferentes cenários epidemiológicos são necessáriosOBJECTIVE: To evaluate the accuracy of determining specific IgA to HspX recombinant antigen in pleural fluid and serum samples for the diagnosis of pleural tuberculosis in patients with pleural effusion. METHODS: This was a cross-sectional study. Serum and pleural fluid samples of patients with pleural effusion and suspected of having pleural tuberculosis were tested with indirect ELISA in order to determine the optical density of specific IgA to HspX. RESULTS: We evaluated serum and pleural fluid samples from 132 patients: 97 diagnosed with pleural tuberculosis (study group and 35 diagnosed with pleural effusion due to other causes (control group. The determination of IgA in pleural fluid satisfactorily discriminated between pleural tuberculosis patients and control patients. The sensitivity of the test in pleural fluid and in serum was 69% and 30

  10. Evaluation of the immune response to CRA and FRA recombinant antigens of Trypanosoma cruzi in C57BL/6 mice Avaliação da resposta imune em camundongos C57BL/6 imunizados com os antígenos recombinantes CRA e FRA de Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Valéria Rêgo Alves Pereira

    2003-07-01

    Full Text Available Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [³H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.As respostas imune humoral e celular foram avaliadas em 44 camundongos C57Bl/6 imunizados com os antígenos recombinantes CRA e FRA de Trypanosoma cruzi. Ambos antígenos induziram reação de hipersensibilidade do tipo imediato. Os níveis de IgG1, IgG2a, IgG2b e IgG3 foram elevados nos camundongos imunizados com CRA. IgG3 foi o isotipo predominante. Nenhuma diferença nos níveis de anticorpos foi observada em camundongos imunizados com FRA em relação aos animais controle. No entanto, ambos antígenos foram capazes de induzir proliferação de linfócitos em camundongos imunizados. Diferenças significativas foram observadas entre a incorporação da timidina - [³H] pelas células esplênicas estimuladas com CRA ou FRA e o grupo controle. Esses resultados sugerem que CRA e FRA poderão estar envolvidos nos mecanismos de resistência à infecção pelo Trypanosoma cruzi.

  11. Safety and efficacy of a single bolus administration of recombinant factor VIIa in liver transplantation due to chronic liver disease

    NARCIS (Netherlands)

    Planinsic, RM; van der Meer, J; Testa, G; Grande, L; Candela, A; Porte, RJ; Ghobrial, RM; Isoniemi, H; Schelde, PB; Erhardtsen, E; Klintmalm, G; Emre, S

    Orthotopic liver transplantation (OLT) can be associated with excessive blood loss. As a result, there may be increased risk of adverse outcomes. Activated recombinant factor VII (rFVIIa) has demonstrated the ability to improve hemostasis in a variety of disorders; however there has been a limited

  12. Safety and efficacy of a single bolus administration of recombinant factor VIIa in liver transplantation due to chronic liver disease

    NARCIS (Netherlands)

    Planinsic, RM; van der Meer, J; Testa, G; Grande, L; Candela, A; Porte, RJ; Ghobrial, RM; Isoniemi, H; Schelde, PB; Erhardtsen, E; Klintmalm, G; Emre, S

    2005-01-01

    Orthotopic liver transplantation (OLT) can be associated with excessive blood loss. As a result, there may be increased risk of adverse outcomes. Activated recombinant factor VII (rFVIIa) has demonstrated the ability to improve hemostasis in a variety of disorders; however there has been a limited a

  13. Factor VIIa response to a fat-rich meal does not depend on fatty acid composition: A randomized controlled trial

    NARCIS (Netherlands)

    Mennen, L.; Maat, M. de; Meijer, G.; Zock, P.; Grobbee, D.; Kok, F.; Kluft, C.; Schouten, E.

    1998-01-01

    A fat-rich meal increases activated factor VII (FVIIa), but it is not clear whether this increase depends on the fatty acid composition of the meal. Therefore, we studied the FVIIa response to fat-rich meals with different fatty acid composition in a randomized controlled crossover trial and investi

  14. Partial agonism through a zinc-Ion switch constructed between transmembrane domains III and VII in the tachykinin NK(1) receptor

    DEFF Research Database (Denmark)

    Holst, B; Elling, C E; Schwartz, T W

    2000-01-01

    Partly due to lack of detailed knowledge of the molecular recognition of ligands the structural basis for partial versus full agonism is not known. In the beta(2)-adrenergic receptor the agonist binding site has previously been structurally and functionally exchanged with an activating metal......-ion site located between AspIII:08-or a His residue introduced at this position in transmembrane domain (TM)-III-and a Cys residue substituted for AsnVII:06 in TM-VII. Here, this interhelical, bidentate metal-ion site is without loss of Zn(2+) affinity transferred to the tachykinin NK(1) receptor....... In contrast to the similarly mutated beta(2)-adrenergic receptor, signal transduction-i.e., inositol phosphate turnover-could be stimulated by both Zn(2+) and by the natural agonist, Substance P in the mutated NK(1) receptor. The metal-ion acted as a 25% partial agonist through binding to the bidentate zinc...

  15. The high-energy multi-group HEST1.0 library based on ENDF/B-VII.0: development, verification and preliminary application

    Science.gov (United States)

    Wu, Jun; Chen, Yi-Xue; Wang, Wei-Jin; Yin, Wen; Liang, Tian-Jiao; Jia, Xue-Jun

    2012-03-01

    ENDF/B-VII.0, which was released by the USA Cross Section Evaluation Working Group (CSEWG) in December 2006, was demonstrated to perform much better than previous ENDF evaluations over a broad range of benchmark experiments. A high-energy (up to 150 MeV) multi-group library set named HEST1.0 with 253-neutron and 48-photon groups has been developed based on ENDF/B-VII.0 using the NJOY code. This paper provides a summary of the procedure to produce the library set and a detailed description of the verification of the multi-group library set by several shielding benchmark devices, in particular for high-energy neutron data. In addition, the first application of HEST1.0 to the shielding design of the China Spallation Neutron Source (CSNS) is demonstrated.

  16. Juan Bautista Vázquez “el Viejo” y Gaspar Núñez Delgado al servicio del VII duque de Medina Sidonia (1575-1576

    Directory of Open Access Journals (Sweden)

    Cruz Isidoro, Fernando

    2012-09-01

    Full Text Available For the chapels in his palaces in Seville and San Lucar, the VII duke of Medina Sidonia ordered several works from the sculptors Juan Bautista Vázquez “the Elder” and Gaspar Núñez Delgado, documented as having been a skilled craftsman in Vázquez’s studio. Both worked on a huge Nativity and other sculptures for the chapels.

    Se documenta la labor de mecenazgo escultórico del VII duque de Medina Sidonia para su capilla de los palacios sevillano y sanluqueño, con el encargo de diversas obras a los escultores Juan Bautista Vázquez “el Viejo” y Gaspar Núñez Delgado, documentado este como oficial en su taller. Ambos realizan un nacimiento monumental y otras obras para las capillas.

  17. DNA-polymorphisms and plasma levels of vascular disease risk factors in Greenland Inuit--is there a relation with the low risk of cardiovascular disease in the Inuit?

    DEFF Research Database (Denmark)

    Maat, M de; Bladbjerg, E-M; Johansen, L G

    1999-01-01

    Greenland Inuit are a population with a low risk of cardiovascular disease. Recently, we stated that frequencies of potentially high risk alleles of the apolipoproteins, fibrinogen, factor V, glycoprotein IIIa and factor VII (FVII) genes have different allele frequencies in the Inuit when compare...

  18. Technological organization of the first Neanderthal societies in the Cantabrian region. Interpreting the lithic industry and fauna from the lowermost levels (V, VI and VII of Lezetxiki cave

    Directory of Open Access Journals (Sweden)

    Talía LAZUÉN

    2012-07-01

    Full Text Available This article studies the technological organization of the first Neanderthal societies that inhabited the Cantabrian region. We use the information provided by the lithic industry excavated in the three lowermost levels (V, VI and VII of Lezetxiki cave. We also explore the other related evidences, especially those inferred from faunal remains. The analysis and explanation of the technical activities fits in a general perspective centered in the production-management-function subsystem.

  19. Mono- and di-halogenated histamine, histidine and carnosine derivatives are potent carbonic anhydrase I, II, VII, XII and XIV activators.

    Science.gov (United States)

    Saada, Mohamed-Chiheb; Vullo, Daniela; Montero, Jean-Louis; Scozzafava, Andrea; Supuran, Claudiu T; Winum, Jean-Yves

    2014-09-01

    Mono- and di-halogenated histamines, l-histidine methyl ester derivatives and carnosine derivatives incorporating chlorine, bromine and iodine were prepared and investigated as activators of five carbonic anhydrase (CA, EC 4.2.1.1) isoforms, the cytosolic hCA I, II and VII, and the transmembrane hCA XII and XIV. All of them were activated in a diverse manner by the investigated compounds, with a distinct activation profile.

  20. Thermal conductivity of ice VII using the time-domain thermo-reflectance method in the diamond anvil cell: Implications for the icy planetary bodies

    Science.gov (United States)

    Chen, B.; Cahill, D. G.; Bartov, G.; Li, J.

    2008-12-01

    As a planetary body ages, the heat trapped and generated in its interior escapes to the surface. Thermal conductivity is a fundamental parameter that governs the thermal evolution and internal dynamics of the planetary body. Due to exceedingly small sample size under high pressure, measuring the thermal conductivity of compressed solids is challenging. Here we report new experimental data on the thermal conductivity of liquid H2O and ice VII up to 11 GPa and at 300 K, using the time-domain thermo- reflectance technique (TDTR) and the diamond anvil cell. The measurements were carried out at the Material Research Laboratory, University of Illinois. We load ruby balls as the pressure marker and a mica sheet as a thermal insulating layer. A thin film of aluminum (Al) is coated on the mica sheet and served as a transducer (Antonelli et al., 2006). A short optical pump pulse with duration ~ 100 fs and energy ~ 1 nJ is focused to a ~10 micron-diameter spot on the Al film, raising its temperature by several degrees Kelvin, which in turn causes a slight change in reflectivity. Over the next few nanoseconds following the absorption of the pulse, the Al film cools via heat conduction through the film itself, across the interface, and into the sample. From time-dependent measurement of reflectivity, we can extract the value of the thermal conductivity of the sample by modeling one-dimensional heat flow. With increasing pressure, liquid H2O crystallized into multiple grains of tetragonal ice VI. Upon further compression to ~ 3.3 GPa, the grain boundaries disappeared due to the formation of the cubic ice VII. We have determined the effect of pressure on the thermal conductivity of ice VII between 3 and 11 GPa. We will discuss the implications of our data for the thermal evolution of icy planetary bodies where ice VII may be a significant component.

  1. 77 FR 29633 - Alta Wind VII, LLC, Alta Wind IX, LLC, Alta Wind X, LLC, Alta Wind XI, LLC, Alta Wind XII, LLC...

    Science.gov (United States)

    2012-05-18

    ... [Federal Register Volume 77, Number 97 (Friday, May 18, 2012)] [Notices] [Page 29633] [FR Doc No: 2012-12075] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL12-68-000] Alta Wind VII, LLC, Alta Wind IX, LLC, Alta Wind X, LLC, Alta Wind XI, LLC, Alta Wind XII, LLC, Alta Wind XIII, LLC, Alta Wind XIV, LLC, Alta Wind XV, LLC...

  2. ENDF-6 Formats Manual Data Formats and Procedures for the Evaluated Nuclear Data File ENDF/B-VI and ENDF/B-VII

    Energy Technology Data Exchange (ETDEWEB)

    Herman, M.; Members of the Cross Sections Evaluation Working Group

    2009-06-01

    In December 2006, the Cross Section Evaluation Working Group (CSEWG) of the United States released the new ENDF/B-VII.0 library. This represented considerable achievement as it was the 1st major release since 1990 when ENDF/B-VI has been made publicly available. The two libraries have been released in the same format, ENDF-6, which has been originally developed for the ENDF/B-VI library. In the early stage of work on the VII-th generation of the library CSEWG made important decision to use the same formats. This decision was adopted even though it was argued that it would be timely to modernize the formats and several interesting ideas were proposed. After careful deliberation CSEWG concluded that actual implementation would require considerable resources needed to modify processing codes and to guarantee high quality of the files processed by these codes. In view of this the idea of format modernization has been postponed and ENDF-6 format was adopted for the new ENDF/B-VII library. In several other areas related to ENDF we made our best to move beyond established tradition and achieve maximum modernization. Thus, the 'Big Paper' on ENDF/B-VII.0 has been published, also in December 2006, as the Special Issue of Nuclear Data Sheets 107 (1996) 2931-3060. The new web retrieval and plotting system for ENDF-6 formatted data, Sigma, was developed by the NNDC and released in 2007. Extensive paper has been published on the advanced tool for nuclear reaction data evaluation, EMPIRE, in 2007. This effort was complemented with release of updated set of ENDF checking codes in 2009. As the final item on this list, major revision of ENDF-6 Formats Manual was made. This work started in 2006 and came to fruition in 2009 as documented in the present report.

  3. Technological organization of the first Neanderthal societies in the Cantabrian region. Interpreting the lithic industry and fauna from the lowermost levels (V, VI and VII) of Lezetxiki cave

    OpenAIRE

    Talía LAZUÉN; Altuna, Jesús

    2011-01-01

    This article studies the technological organization of the first Neanderthal societies that inhabited the Cantabrian region. We use the information provided by the lithic industry excavated in the three lowermost levels (V, VI and VII) of Lezetxiki cave. We also explore the other related evidences, especially those inferred from faunal remains. The analysis and explanation of the technical activities fits in a general perspective centered in the production-management-function subsystem. En...

  4. La seconda volta. Arte e artisti attorno a Carlo V e Clemente VII a Bologna nel 1532-33

    Directory of Open Access Journals (Sweden)

    Giovanni Sassu

    2012-06-01

    Full Text Available Dopo l’incoronazione del 1530, Clemente VII e Carlo V scelgono di nuovo Bologna, seconda città dello Stato Pontificio, come sede di un nuovo incontro tra il 1532 ed il 1533. In questo contesto, le arti figurative, attraverso personalità come Alfonso Lombardi e Tiziano Vecellio, giocano un ruolo di eccezionale rilevanza, chiamate a dare forma alle nuove esigenze rappresentative del potere asburgico.Après le couronnement de 1530, Clément VII et Charles-Quint choisissent à nouveau Bologne, seconde ville des États pontificaux, comme lieu d’une autre rencontre, en 1532/33. Dans ce contexte, les arts figuratifs, à travers des personnalités tels Alfonso Lombardi et Tiziano Vecellio, jouent un rôle d’une importance exceptionnelle, car ils sont appelés à donner forme aux nouvelles exigences représentatives du pouvoir habsbourgeois.Después de la coronación del 1530, Clemente VII y Carlos V eligen de nuevo Bolonia, segunda ciudad del Estado pontificio, como sede de otro encuentro, en 1532/33. En este contexto, las artes figurativas, mediante personalidades como Alfonso Lombardi y Tiziano Vecellio, desempeñan un papel destacado, ya que les corresponderá dar forma a las nuevas exigencias representativas del poder Habsburgo.

  5. The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum.

    Science.gov (United States)

    Gandía, Mónica; Harries, Eleonora; Marcos, Jose F

    2014-06-01

    Chitin is an essential component of the fungal cell wall and a potential target in the development of new antifungal compounds, due to its presence in fungi and not in plants or vertebrates. Chitin synthase genes (chs) constitute a complex family in filamentous fungi and are involved in fungal development, morphogenesis, pathogenesis and virulence. In this study, additional chs genes in the citrus postharvest pathogen Penicillium digitatum have been identified. Comparative analyses included each PdChs in each one of the classes I to VII previously established, and support the grouping of these into three divisions. Disruption of the gene coding PdChsVII, which contains a short version of a myosin motor domain, has been achieved by using Agrobacterium tumefaciens-mediated transformation and revealed its role in the life cycle of the fungus. Disruption strains were viable but showed reduced growth and conidia production. Moreover, Pdchs mutants developed morphological defects as balloon-like enlarged cells and increased chitin content, indicative of an altered cell wall structure. Gene disruption also increased susceptibility to antifungal compounds such as calcofluor white (CFW), sodium dodecyl sulfate (SDS), hydroxide peroxide (H2O2) and commercial fungicides, but significantly no change was observed in the sensitivity to antifungal peptides. The PdchsVII mutants were able to infect citrus fruit and produced tissue maceration, although had reduced virulence and most importantly were greatly impaired in the production of visible mycelium and conidia on the fruit.

  6. The O VII X-ray forest toward Markarian 421: Consistency between XMM-Newton and Chandra

    CERN Document Server

    Kaastra, J S; Den Herder, J W A; Paerels, F B S; De Plaa, J; Rasmussen, A P; De Vries, C P

    2006-01-01

    Recently the first detections of highly ionised gas associated with two Warm-Hot Intergalactic Medium (WHIM) filaments have been reported. The evidence is based on X-ray absorption lines due to O VII and other ions observed by Chandra towards the bright blazar Mrk 421. We investigate the robustness of this detection by a re-analysis of the original Chandra LETGS spectra, the analysis of a large set of XMM-Newton RGS spectra of Mrk 421, and additional Chandra observations. We address the reliability of individual spectral features belonging to the absorption components, and assess the significance of the detection of these components. We also use Monte Carlo simulations of spectra. We confirm the apparent strength of several features in the Chandra spectra, but demonstrate that they are statistically not significant. This decreased significance is due to the number of redshift trials that are made and that are not taken into account in the original discovery paper. Therefore these features must be attributed t...

  7. La sacralización del rey. Fernando VII, la insurgencia novohispana y el derecho divino de los reyes

    Directory of Open Access Journals (Sweden)

    Landavazo, Marco Antonio

    2001-04-01

    Full Text Available This article analyzes what the author called the «process of sacralization» of King Ferdinand VII’s image in New Spain, and its political and ideological consequences in the Mexican Independence War. The people’s believes, values and attitudes towards the Monarchy were an essential part of the local political culture. These played a decisive role in the course of political and social events of the period.

    En este artículo se describe y analiza lo que he llamado el proceso de sacralización de la figura del rey Fernando VII en Nueva España y las implicaciones políticas e ideológicas que ello tuvo en el curso de la guerra de independencia mexicana, como una forma de mostrar que las creencias, los valores y las actitudes que los novohispanos poseían en torno a la institución monárquica jugaban un papel fundamental en la definición de la cultura política de la época, y condicionaron de hecho, en buena medida, el curso de los acontecimientos más relevantes del periodo.

  8. PENGARUH MEDIA PEMBELAJARAN ADOBE FLASH PLAYER DAN INFOKUS TERHADAP PEMAHAMAN KONSEP MATEMATIS SISWA KELAS VII SMP PGRI 11 PALEMBANG

    Directory of Open Access Journals (Sweden)

    Destinar Destinar

    2016-08-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui ada tidaknya pengaruh media pembelajaran Adobe Flash Player dan Infokus terhadap pemahaman konsep matematis siswa Kelas VII. Metode yang digunakan dalam penelitian ini adalah metode eksperimen, yang terdiri dari 3 kelompok yaitu kelompok kelas yang diajar dengan Adobe Flash Player dan Infokus, kelompok kelas yang diajar hanya dengan infokus dan kelompok kelas yang diajar tanpa media. Setelah itu ketiga kelompok tersebut diberikan tes akhir untuk mengetahui pemahaman konsep matematis dari masing-masing kelompok. Dari hasil tes akhir diperoleh Dari hasil tes akhir diperoleh rata-rata pemahaman konsep matematis kelompok yang diajar dengan Adobe Flash Player dan Infokus secara signifikan lebih tinggi dari pada rata-rata pemahaman konsep matematis kelompok yang diajar dengan Infokus. Rata-rata pemahaman konsep matematis kelompok yang diajar dengan Adobe Flash Player dan Infokus secara signifikan lebih tinggi dari pada rata-rata pemahaman konsep matematis kelompok yang diajar dengan tanpa media. Rata-rata pemahaman konsep matematis kelompok yang diajar dengan Infokus secara signifikan lebih tinggi dari pada rata-rata pemahaman konsep matematis kelompok yang diajar dengan tanpa media. Sehingga dapat disimpulkan terdapat pengaruh yang signifikan pemahaman konsep matematis siswa ditinjau dari media pembelajaran yang digunakan.  Kata Kunci : Adobe Flash Player, Infokus, Pemahaman Konsep Matematis. 

  9. In-body tissue-engineered aortic valve (Biovalve type VII) architecture based on 3D printer molding.

    Science.gov (United States)

    Nakayama, Yasuhide; Takewa, Yoshiaki; Sumikura, Hirohito; Yamanami, Masashi; Matsui, Yuichi; Oie, Tomonori; Kishimoto, Yuichiro; Arakawa, Mamoru; Ohmuma, Kentaro; Tajikawa, Tsutomu; Kanda, Keiichi; Tatsumi, Eisuke

    2015-01-01

    In-body tissue architecture--a novel and practical regeneration medicine technology--can be used to prepare a completely autologous heart valve, based on the shape of a mold. In this study, a three-dimensional (3D) printer was used to produce the molds. A 3D printer can easily reproduce the 3D-shape and size of native heart valves within several processing hours. For a tri-leaflet, valved conduit with a sinus of Valsalva (Biovalve type VII), the mold was assembled using two conduit parts and three sinus parts produced by the 3D printer. Biovalves were generated from completely autologous connective tissue, containing collagen and fibroblasts, within 2 months following the subcutaneous embedding of the molds (success rate, 27/30). In vitro evaluation, using a pulsatile circulation circuit, showed excellent valvular function with a durability of at least 10 days. Interposed between two expanded polytetrafluoroethylene grafts, the Biovalves (N = 3) were implanted in goats through an apico-aortic bypass procedure. Postoperative echocardiography showed smooth movement of the leaflets with minimal regurgitation under systemic circulation. After 1 month of implantation, smooth white leaflets were observed with minimal thrombus formation. Functional, autologous, 3D-shaped heart valves with clinical application potential were formed following in-body embedding of specially designed molds that were created within several hours by 3D printer. © 2014 Wiley Periodicals, Inc.

  10. MCNP Super Lattice Method for VHTR ORIGEN2.2 Nuclear Library Improvement Based on ENDF/B-VII

    Energy Technology Data Exchange (ETDEWEB)

    G. S. Chang; J. R. Parry

    2010-10-01

    The advanced Very High Temperature gas-cooled Reactor (VHTR) achieves simplification of safety through reliance on innovative features and passive systems. One of the VHTRs innovative features is the reliance on ceramic-coated fuel particles to retain the fission products under extreme accident conditions. The effect of the random fuel kernel distribution in the fuel prismatic block creates a double-heterogeneous lattice, which needs to be addressed through the use of the newly developed prismatic super Kernel-by-Kernel Fuel (KbKF) lattice model method. Based on the new ENDF/B-VII nuclear cross section evaluated data, the developed KbKF super lattice model was then used with MCNP to calculate the material isotopes neutron reaction rates, such as, (n,?); (n,n’); (n,2n’); (n,f); (n,p); (n,?). Then, the MCNP-calculated results are rearranged to generate a set of new libraries “VHTRXS.lib,” for the ORIGEN2.2 isotopes depletion and build-up analysis code. The libraries contain one group cross section data for the structural light elements, actinides, and fission products that can be applied in the VHTR related fuel burnup and material transmutation analysis codes. The efficiency and ease of use of the MCNP method to generate and update the ORIGEN2.2 one-group spectrum weighed cross section library for VHTR was demonstrated.

  11. Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters.

    Science.gov (United States)

    Vorobyev, Artem; Ujiie, Hideyuki; Recke, Andreas; Buijsrogge, Jacqueline J A; Jonkman, Marcel F; Pas, Hendri H; Iwata, Hiroaki; Hashimoto, Takashi; Kim, Soo-Chan; Hoon Kim, Jong; Groves, Richard; Samavedam, Unni; Gupta, Yask; Schmidt, Enno; Zillikens, Detlef; Shimizu, Hiroshi; Ludwig, Ralf J

    2015-06-01

    Epidermolysis bullosa acquisita (EBA) is an autoimmune blistering disease of the skin and mucous membranes, characterized by autoantibodies against type VII collagen (COL7), a major component of anchoring fibrils. Different clinical EBA phenotypes are described, including mechanobullous and inflammatory variants. Most EBA patients' sera react with epitopes located within the non-collagenous 1 (NC1) domain of human COL7. However, it has remained unclear whether antibody binding to these different epitopes is pathogenically relevant. To address this issue, we generated recombinant proteins covering the entire NC1 domain. IgG reactivity with these proteins was analyzed in sera of 69 EBA patients. Most recognized clusters of epitopes throughout the NC1 domain. No correlation was detected between antibody specificity and clinical phenotype. To study the pathogenicity of antibodies specific to different NC1 subdomains, rabbit antibodies were generated. All these antibodies caused dermal-epidermal separation ex vivo. Antibodies against two of these subdomains were injected into mice carrying null mutations of mouse COL7 and the human COL7 transgene and induced subepidermal blisters. We here document that autoantibodies to COL7, independent of the targeted epitopes, induce blisters both ex vivo and in vivo. In addition, using COL7-humanized mice, we provide in vivo evidence of pathogenicity of autoantibodies binding to human COL7.

  12. Population dynamics of the ions SVI, ClVII and ArVIII interacting with solid surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Nedeljkovic, N.N. [Faculty of Physics, University of Belgrade, P.O. Box 368, Belgrade (Serbia)], E-mail: hekata@ff.bg.ac.yu; Majkic, M.D.; Galijas, S.M.D.; Mitrovic, S.B. [Faculty of Physics, University of Belgrade, P.O. Box 368, Belgrade (Serbia)

    2008-08-30

    We used the recently developed time-symmetrized, two-state vector model to investigate the intermediate stages of the electron capture into the Rydberg states (n{sub A}>>1,l{sub A}=0-3, m{sub A}=0) of multiply charged ions SVI, ClVII and ArVIII, with polarized core charges Z=6, 7 and 8, respectively, escaping solid surfaces at low velocities. Within the framework of the model, the two wave functions are used to describe the system at intermediate stages; the corresponding probabilities and rates are based on the calculation of the mixed flux. The simple analytical formulae derived for the neutralization rates enable us to analyze the localization of the process and to calculate the neutralization distances. In the pointlike core approximation, the rates are comparable with the available coupled-angular-mode results. The neutralization distances follow the classical predictions for all considered Rydberg states with exception of the states with critical quantum numbers n{sub A}=n{sub c}, mainly populated via tunneling mechanism; the corresponding values are larger than it is predicted by the classical overbarrier model. Inclusion of core polarization significantly reduces the neutralization distances.

  13. Finding strategic communication & diverse leadership in the ancient world: The case of Queen Cleopatra VII, the last pharaoh of Egypt

    Directory of Open Access Journals (Sweden)

    Shannon A. Bowen

    2016-12-01

    Full Text Available The reign of Queen Cleopatra VII of Egypt offers a rare historical glimpse into ancient communication activities and how communication was used by the world’s last pharaoh. This historical research reveals how Cleopatra used public relations and strategic communication activities to advance both her reign as a lone woman sovereign and the interests of the Egyptian empire. Cleopatra was perhaps the first woman sovereign in history to rule alone for a period of over a decade. Her leadership showed skilled use of public communication and diplomacy with which she forestalled the fall of Egypt to Rome. A grounded theory approach allowed data to emerge naturally, surrounding communication activities, and then for a theoretical framework to be imposed that could explain the common themes in that data. This research compares Cleopatra’s activities with public relations theory using the classic four models of public relations to classify public communication. Findings show that Cleopatra used research in asymmetrical public relations and symmetrical relationship building, also including public diplomacy, public information, and press agentry/pseudo-event mastery. Careful examination of literature sources led to the conclusion that Cleopatra was a truly advanced, research-based, relationship-focused, and international policy-oriented lone woman ruler who was a supremely strategic communicator.

  14. STUDIES ON PERMEABILITY OF MEMBRANES : VII. CONDUCTIVITY OF ELECTROLYTES WITHIN THE MEMBRANE.

    Science.gov (United States)

    Green, A A; Weech, A A; Michaelis, L

    1929-01-20

    Two methods of measuring the electrical conductivity of the dried collodion membrane in contact with an electrolyte solution are described and the results of such measurements with different electrolytes in different ranges of concentration recorded. Some of the difficulties encountered in making these measurements are outlined. Of special interest was the fact that each membrane with each electrolyte showed a maximum level of resistance at a certain point in the dilution scale, a level which was not surpassed by further dilution. It is believed that this level was fixed by the collodion itself rather than by the contiguous electrolyte solution. Its existence limited the results available for reasonable interpretation. In relatively concentrated solutions the conductivity was shown to be approximately proportional to the concentration. With different electrolytes in the same concentration it was shown that the conductivities varied much more than in simple solutions without a membrane and that they fell in the order HCl > KCl > NaCl > LiCl. A method was described whereby the electrolyte content of a membrane in contact with different chloride solutions could be determined. It was shown that a membrane saturated with either 0.5 N HCl or 0.5 N KCl had practically the same total electrolyte content whereas the same membrane in contact with 0.5 N LiCl contained only half the quantity. These results were used in interpreting the conductivity data, the evidence presented strongly suggesting that two factors are operative in causing the widely divergent conductivities recorded with different electrolytes. The first factor depended on the quantity of electrolyte which can enter the membrane pores, a quantity dependent on the size of the pores and the volume of the larger of the two hydrated ions of the electrolyte. This factor was the chief one in determining the difference in conductivity between KCl and LiCl. The second factor was concerned with differences in the

  15. Experimental arterial thrombosis in genetically or diet induced hyperlipidemia in rats--role of vitamin K-dependent clotting factors and prevention by low-intensity oral anticoagulation.

    Science.gov (United States)

    De Curtis, A; D'Adamo, M C; Amore, C; Polishchuck, R; Castelnuovo, A D; Donati, M B; Iacoviello, L

    2001-12-01

    To investigate the relationship among lipids, coagulation and thrombosis in the absence of atherosclerosis, spontaneous or dietary-induced hyperlipidemic (FHL) rats were studied. FHL showed higher levels of coagulation factors VII, IX, X, VIII and XII and a shortening of the occlusion time (OT) of an artificial arterial prosthesis as compared with normolipidemic (FNL) animals. Damage of abdominal aorta of FHL was followed by increased fibrin deposition in the vascular intima as compared to FNL. After 5 months of cholesterol-rich diet FNL showed increased cholesterol, triglycerides and factor II, VII, IX, X, XII levels. A significant shortening of the OT and increased fibrin deposition was also observed. Two-month diet withdrawal restored the initial condition. Warfarin treatment, at a dose decreasing vitamin K-dependent factor to levels found in FNL, prolonged the OT and reduced fibrin deposition, without modifying F XII or changing lipid profile. An increase in the activated form of F VII was observed. In contrast, no difference was found in F VII clearance. High lipid levels favour the process of thrombus formation by increasing the activation of vitamin K-dependent coagulation factors. Low-dose warfarin treatment reverts the prothrombotic effect of hyperlipidemia.

  16. Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins

    Directory of Open Access Journals (Sweden)

    Ken-ichi eLee

    2015-02-01

    Full Text Available GI-VII-6 is a chromosomally integrated multidrug resistance genomic island harbored by a specific clone of Salmonella enterica serovar Typhimurium (S. Typhimurium. It contains a gene encoding CMY-2 β-lactamase (blaCMY-2, and therefore contributes to extended-spectrum cephalosporin resistance. To elucidate the significance of GI-VII-6 on adaptive evolution, spontaneous mutants of S. Typhimurium strain L-3553 were selected on plates containing cefotaxime (CTX. The concentrations of CTX were higher than its minimum inhibition concentration to the parent strain. The mutants appeared on the plates containing 12.5 and 25 μg/ml CTX at a frequency of 10−6 and 10−8, respectively. No colonies were observed at higher CTX concentrations. The copy number of blaCMY-2 increased up to 85 per genome in the mutants, while the parent strain contains one copy of that in the chromosome. This elevation was accompanied by increased amount of transcription. The blaCMY-2 copy number in the mutants drastically decreased in the absence of antibiotic selection pressure. Southern hybridization analysis and short-read mapping indicated that the entire 125 kb GI-VII-6 or parts of it were tandemly amplified. GI-VII-6 amplification occurred at its original position, although it also transposed to other locations in the genome in some mutants, including an endogenous plasmid in some of the mutants, leading to the amplification of GI-VII-6 at different loci. Insertion sequences were observed at the junction of the amplified regions in the mutants, suggesting their significant roles in the transposition and amplification. Plasmid copy number in the selected mutants was 1.4 to 4.4 times higher than that of the parent strain. These data suggest that transposition and amplification of the blaCMY-2-containing region, along with the copy number variation of the plasmid, contributed to the extensive amplification of blaCMY-2 and increased resistance to CTX.

  17. PRODUCCIÓN DE LA PROTEÍNA DE CHOQUE TÉRMICO HSC70 RECOMBINANTE EN Escherichia Coli BL21(DE3 PARA GENERAR ANTICUERPOS POLICLONALES PRODUCTION OF RECOMBINANT HEAT SHOCK PROTEIN HSC70 IN Escherinchia coli BL21(DE3 TO OBTAIN POLYCLONAL ANTIBODIES

    Directory of Open Access Journals (Sweden)

    Rocio Cely Castro

    2006-07-01

    Full Text Available Antecedentes. Los organismos vivos responden al estrés aumentando la síntesis de proteínas. El estrés por choque térmico ha sido el más estudiado y las proteínas que se inducen, se han denominado genéricamente como proteínas de choque térmico. Objetivo.En este trabajo se establecieron las condiciones óptimas de producción de la proteína HSC70, expresada en E.coli BL21 (DE3 y de los anticuerpos policlonales que permitan identificarla. Material y métodos. Mediante varios ensayos se establecieron las concentraciones óptimas del agente inductor isopropil- -D-thiogalacto­piranosido (PTG, del inóculo bacteriano, de empleo de los plásmidos de expresión pET-3a y pET-28a (+; y el método más eficiente para la recuperación de las formas soluble o insoluble de la proteína y de anticuerpos policlonales que la identifiquen. Resultados. Encontramos que el inóculo de cinco colonias con IPTG (2mM en tubos con cinco mililitros de medio modificado e incubadas por 24 horas a 37°C con agitación constante (200 r.p.m y tratamiento de sonicación, produce el mejor rendimiento de HSC70. La calidad de la proteína inducida se estableció mediante “Western blotting”. Conclusión. La proteína recombinante así obtenida permitió, generar anticuerpos policlonales que a su vez permiten detectar la proteína HSC70 natural en la membrana citoplasmática de diferentes células por inmunofluorescencia, en ELISA, en Western Blot y en pruebas de bloqueo de infección de rotavirus.Background. Live organisms respond against stressing conditions raising the production of stress proteins. The heat shock stress is a very well kwon condition that produces heat shock proteins. Objective. In this work we determined the optimum conditions to produce HSC70 protein in E. coli BL12 (DE3 under stress conditions, and the best way to obtain its polyclonal antibodies. Materials and methods. The best concen­trations for isopropyl- ß -D

  18. Pro-hepcidina, su relación con indicadores del metabolismo del hierro y de inflamación en pacientes hemodializados tratados o no con eritropoyetina recombinante Pro-hepcidin, its relation with indicators of iron metabolism and of inflammation in patients hemodialyzed treated or not with recombinant erythropoietin

    Directory of Open Access Journals (Sweden)

    Y. Barrios

    2010-08-01

    Full Text Available La hepcidina, péptido antimicrobiano, cuya síntesis se encuentra regulada por el estado del hierro e inflamación, juega un importante papel en la homeostasis del hierro en pacientes hemodializados (HD. Es medida a través de la prohepcidina sérica. Objetivo: Determinar los niveles séricos de prohepcidina y su relación con ferritina sérica, proteína C reactiva (PCR y albúmina en pacientes HD tratados o no con eritropoyetina recombinante (EPO, que asistieron a un Centro de Salud del Estado Carabobo-Venezuela. Metodología: Esta investigación es de tipo descriptiva, correlacional y de campo, cuya muestra estuvo conformada por 71 pacientes HD; 57 de éstos tratados con EPO. Se determinó prohepcidina sérica, ferritina, hemoglobina, hematocrito, PCR y albúmina. Se definió anemia (hemoglobina 10 mg/L. Resultados: El promedio para la prohepcidina fue de 397,5 ng/mL. Se observó un alto porcentaje de anemia (87,3% y un 22,5% de pacientes con ferritina sérica baja. No se observaron diferencias estadísticamente significativas para ferritina, albúmina, PCR y prohepcidina, entre pacientes con y sin tratamiento con EPO. Solo la variable PCR se correlaciona significativamente (rho = 0,276; p =0,020, con prohepcidina. Conclusión: Pacientes HD presentan niveles elevados de prohepcidina, esto pudiera deberse al proceso inflamatorio frecuentemente observado en estos pacientes y no al estado de hierro medido a través de los niveles de ferritina sérica.Hepcidin, an antimicrobial peptide which synthesis is regulated by iron status and inflammation, plays an important role in iron homeostasis in hemodialysed (HD patients. It is measured by measuring serum prohepcidin. Objective: To determine serum prohepcidin levels and their relationship with serum ferritin, C reactive protein (CRP, and albumin in HD patients treated or not with recombinant erythropoietin (EPO that attended the Health Centre of the Carabobo State in Venezuela. Methodology: This is

  19. Leptospirosis serosurvey in bovines from Brazilian Pantanal using IGG ELISA with recombinant protein LipL32 and microscopic agglutination test Sorodiagnóstico de leptospirose em bovinos do Pantanal brasileiro utilizando ELISA IgG com proteína recombinante LipL32 e soroaglutinação microscópica

    Directory of Open Access Journals (Sweden)

    Renata Graça Pinto Tomich

    2007-12-01

    Full Text Available This investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region's climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutination test (MAT and additionally contribute to the knowledge of the distribution of the one of most important worldwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an important ecological preserved area, where cattle constitute not only the most important economic resource but also the major activity compatible of the conservation of natural resource of the region. Out of 282 samples of cattle serum analyzed, 143 (50.71% were positive in MAT. The serovar Hardjo (genotypic Hardjoprajitno and Hardjobovis, Wolffi and Ballum showed the largest frequency of reactive samples. In the IgG ELISA rLipL32, 161 samples (57.09% were positive. This result was higher than obtained by MAT (pEste estudo foi realizado no Pantanal brasileiro: região que apresenta importante biodiversidade. As condições de clima, hidrologia e geomorfologia dessa região, bem como a existência de grande variedade de espécies animais silvestres, favorecem a manutenção da Leptospira no meio ambiente. O objetivo desse estudo foi avaliar o ELISA IgG com proteína recombinante LipL32 em comparação com a soroaglutinação microscópica (SAM para o diagnóstico sorológico de Leptospira. Adicionalmente, contribuir para o conhecimento da distribuição da leptospirose bovina, uma das mais importantes zoonoses mundialmente distribuída. Foi avaliada a soropositividade para essa bactéria em rebanhos bovinos de corte da região do Pantanal, uma área onde o bovino constitui não apenas o recurso econômico mais importante

  20. Resposta superovulatória e viabilidade de embriões de doadoras Nelore submetidas a pré-tratamento com Somatotropina Recombinante Bovina (rbST Superovulatory response and embryo viability of Nelore donors undergo the pre-treatment with Bovine Somatrotopin Recombinant (rbST

    Directory of Open Access Journals (Sweden)

    Bruno Henrique Araújo Andrade

    2011-12-01

    Full Text Available Objetivou-se com este estudo avaliar a influência da associação de duas doses contínuas da Somatotropina Recombinante Bovina (rbST a um protocolo de superovulação sobre a quantidade e a qualidade dos embriões produzidos, além da taxa de gestação de embriões provenientes de doadoras tratadas com a rbST. Foram usadas seis doadoras distribuídas em três tratamentos experimentais. O primeiro, tratamento I (TI, recebeu uma dose de 500mg de rbST no dia 0 do protocolo de sincronização da onda folicular; o segundo, tratamento II (TII, recebeu duas doses de 500mg rbST, a primeira dose 14 dias antes do início do protocolo e a outra no dia do início do protocolo e o terceiro, tratamento zero (T0, serviu de controle. As médias do número de estruturas colhidas, embriões viáveis, taxa de viabilidade, estádio de desenvolvimento e qualidade embrionária não diferiram estatisticamente entre os tratamentos. As taxas de gestação das receptoras foram: TI=55,0% (22/40, TII=60,0% (27/45 e T0=34,4% (11/32. Houve diferença significativa entre o T0 e TII. Conclui-se que a rbST melhorou as taxas de gestação de embriões provenientes de doadoras tratadas com rbST 14 dias antes do início do protocolo e no dia do início do tratamento.The purpose of this study was to evaluate the association of two continuous doses of Recombinant Bovine Somatotropin (rbST, in a protocol of superovulation on the quantity and quality of embryos produced and to evaluate the pregnancy rate of embryos from donors treated with the rbST. Six donors were used divided into three experimental treatments. The first, treatment I (TI, received a dose of 500mg of rbST on day 0 of the protocol of synchronization of follicular wave; the second, treatment II (TII, received two doses of 500mg rbST, the first dose 14 days before the start of the protocol and the other on the start of the protocol and the third, treatment zero (T0, served as control. The means number of structures