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Sample records for f1 progeny production

  1. Efficient method for establishing F1 progeny from wild populations of Anopheles mosquitoes.

    Science.gov (United States)

    Nepomichene, Thiery N; Andrianaivolambo, Lala; Boyer, Sébastien; Bourgouin, Catherine

    2017-01-09

    The changing malaria situation in Madagascar requires additional knowledge on the physiology and behaviour of local mosquito vectors. However, the absence of established colonies for several anopheline species present in Madagascar constitutes a limiting factor. To avoid labour intensive work and uncertainty for success of establishing Anopheles colonies from Malagasy species, field collections of blood-fed females and in-tube forced oviposition were combined to reliably produce large numbers of F1 progeny. Blood-fed females were captured in zebu stables or open zebu parks. Oviposition was induced by enclosing gravid females in eppendorf tubes as initially described for Anopheles funestus. The effect of cold anaesthesia on inducing in-tube forced oviposition and on egg yield was assessed for five Anopheles species, namely Anopheles coustani, An. funestus, Anopheles mascarensis, Anopheles arabiensis and Anopheles squamosus. The production of eggs from in-tube forced oviposition and standard egg laying in cages was compared. For the five anopheline species studied, the in-tube forced oviposition method had different efficacy ranging from 35.6 to 71.1% females willing to lay eggs in tubes. Interestingly, prior anaesthesia increased significantly the proportion of ovipositing females for An. mascarensis. Prior anaesthesia has a marginal effect on the number of eggs produced. However, the overall yield in eggs collected using the in-tube forced oviposition method largely exceeds the number of eggs that can be produced by females free to oviposit in cages. The efficiency of the method allowed the production of F1 progeny in numbers sufficiently large for developing detailed analyses of the five species tested, including behavioural studies, insecticide resistance assessment and molecular characterization, as well as vector competence studies. It should be applicable to other anopheline species difficult to colonize.

  2. Inheritance of S-genotypes in Paviot × Kabaasi apricot F1 progenies

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    Zehra Tuğba Murathan

    2016-09-01

    Full Text Available Self-incompatibility plays an important role in the fertilization of fruit species such as apricot. Apricot (Prunus armeniaca L. shows gametophytic self-incompatibility, which is controlled by a multi-allelic S-locus. In this study, S-alleles of 77 F1 progenies derived from Paviot, which is one of the French local cultivars, and Kabaasi, one of the most important Turkish dried apricot cultivars, parents were identified by S-RNase intron regions polymerase chain reaction (PCR amplification and DNA sequencing. The results from the S-allele PCR analysis revealed that the Paviot female parent had an ScS2 genotype and the Kabaasi male parent had S1S9 alleles. Forty-three of the F1 progenies showed self-compatibility allele (Sc by having either ScS9 or ScS1 alleles. Thirty-four of the F1 progenies were self-incompatible by having either S2S1 or S2S9 alleles. The distributions of detected alleles in F1 progenies were determined as follows: ScS1 31.2%, S1S2 27.3%, ScS9 24.7% and S2S9 16.8%. The results from the study are relevant for the data obtained in apricot breeding programmes in the selection of crossing combinations and in the establishment of commercial orchards.

  3. Early growth performance of full-sib Acacia auriculiformis x Acacia mangium F1 hybrid progenies at three different sites

    Science.gov (United States)

    Shah Aimin, Atirah Abdullah; Abdullah, Mohd Zaki; Muhammad, Norwati; Ratnam, Wickneswari

    2014-09-01

    Field trials of 14 full sib Acacia auriculiformis x Acacia mangium F1 hybrid progenies were evaluated for growth performance at three sites (Bintulu, Mentakab and Segamat). Results indicated that there were significant differences (p> 0.05) for diameter breast height (Dbh) and total height (Ht) among the progenies and different sites. Superior progenies have been identified for future tree selection and improvement.

  4. 引进印尼种大珠母贝繁育F1母贝植核育珠研究%Pearl Production from the Pearl Oyster Pinctada Maxima Progeny Stocks Produced by the Breeders Introduced from Indonesia

    Institute of Scientific and Technical Information of China (English)

    谢绍河; 谭楚勤; 邓岳文; 符韶; 梁飞龙; 杜晓东; 林伟财; 王钦贵; 邱壮炮; 方昂松

    2013-01-01

    以印度尼西亚引进大珠母贝亲本繁育的子代F1群体进行植核育珠,育珠贝数量8086只,在海区休养20 d后,统计育珠母贝的成活率与留核率,比较3月与4月植核手术育珠贝的育珠性状差异。结果表明:休养期结束后,育珠贝的成活率与留核率分别为97.57%和90.69%;育珠期3个月时(6月17日)育珠贝的成活率为81.22%;4月植核育珠母贝的留核率与成活率明显高于在3月的植核育珠母贝,但两者之间差异不具统计学意义(P>0.05)。%In March and April of 2013, nucleus-inserting operation was conducted with the donor and host oysters sampled from the progeny stocks produced by the introduced breeders from Indonesia. The total number of host oyster was 8086. Retention rate and survival rate were estimated after the host oysters were recuperated for 20 days in the sea. Survival rates were calculated at culturing times. Differences in retention rate and survival rate of the host oysters treated in March and April were also evaluated. The results showed that mean values of retention rate and survival rate were 97.57% and 90.69%, respectively, after host oysters were recuperated for 20 days in the sea. Mean survival rate of the host oysters was 81.22%, when they were cultured for approximately 3months. The host oysters treated in April showed higher retention rate and survival rate than those treated in March. However, the differences were not significant between the two groups (P>0.05). The present results are helpful for pearl production by pearl oyster Pinctada maxima.

  5. GISH Analysis of Subsequent Progeny Crossed with 2n-gametes of F-1 Oriental-Asiatic Interspecific Hybrid in Lily

    NARCIS (Netherlands)

    Chung, M.Y.; Chung, J.D.; Tuyl, van J.M.; Lim, K.B.

    2009-01-01

    This study was carried out to analyze the chromosome composition and homoeologous recombination of subsequent progenies, derived from cross of Oriental-Asiatic (OA) interspecific hybrid producing 2n-gametes, by GISH technique. GISH analysis of F-1 OA interspecific hybrid '2nOA-001', which produced 2

  6. Molecular marker heterozygosities and genetic distances as correlates of production traits in F1 bovine crosses

    Directory of Open Access Journals (Sweden)

    Daniella Tambasco-Talhari

    2005-01-01

    Full Text Available Several studies have investigated the relationship between heterozygosity, genetic distance and production traits. The objective of the present study was to evaluate the influence of the degree of heterozygosity and genetic distance on growth, carcass and reproductive related features in F1 bovine crosses. We tested 10 polymorphic markers in 330 purebred cattle (Nelore, Canchim, Aberdeen Angus and Simental and 256 crossbred cattle belonging to four crossbred groups. Individual heterozygosities (Hi and multilocus genetic similarity (Dm were estimated and used in correlation analysis against individual phenotypic measurements. Significant (p < 0.05 Hi effects occurred for birth weight, 15 to 18 month weight, hot carcass weight and longissimus rib eye area. The extent to which increased heterozygosity (deltaH in F1 crosses can be predicted from the genetic distance of parental breeds was also investigated using Nei's standard genetic distance (Ds and standard heterozygosity (Hs. High correlations were found between deltaHi, deltaHs and the Ds of the parental breeds. Our results suggest that heterozygosity of the ten molecular markers used in this study may affect live weight during at least one growth phase. Parental genetic distance was a suitable predictor of the degree of progeny heterozygosity.

  7. Mated progeny production is a biomarker of aging in Caenorhabditis elegans.

    Science.gov (United States)

    Pickett, Christopher L; Dietrich, Nicholas; Chen, Junfang; Xiong, Chengjie; Kornfeld, Kerry

    2013-12-09

    The relationships between reproduction and aging are important for understanding the mechanisms of aging and evaluating evolutionary theories of aging. To investigate the effects of progeny production on reproductive and somatic aging, we conducted longitudinal studies of Caenorhabditis elegans hermaphrodites. For mated wild-type animals that were not sperm limited and survived past the end of the reproductive period, high levels of cross-progeny production were positively correlated with delayed reproductive and somatic aging. In this group of animals, individuals that generated more cross progeny also reproduced and lived longer than individuals that generated fewer cross progeny. These results indicate that progeny production does not accelerate reproductive or somatic aging. This longitudinal study demonstrated that cumulative cross progeny production through day four is an early-stage biomarker that is a positive predictor of longevity. Furthermore, in mated animals, high levels of early cross progeny production were positively correlated with high levels of late cross progeny production, indicating that early progeny production does not accelerate reproductive aging. The relationships between progeny production and aging were further evaluated by comparing self-fertile hermaphrodites that generated relatively few self progeny with mated hermaphrodites that generated many cross progeny. The timing of age-related somatic degeneration was similar in these groups, suggesting progeny production does not accelerate somatic aging. These studies rigorously define relationships between progeny production, reproductive aging, and somatic aging and identify new biomarkers of C. elegans aging. These results indicate that some mechanisms or pathways control age-related degeneration of both reproductive and somatic tissues in C. elegans.

  8. Measurement of Inclusive $f_1$(1285) and $f_1$(1420) Production in Z Decays with the DELPHI Detector

    CERN Document Server

    Abdallah, J; Adam, W; Adzic, P; Albrecht, T; Alderweireld, T; Alemany-Fernandez, R; Allmendinger, T; Allport, P P; Amaldi, Ugo; Amapane, N; Amato, S; Anashkin, E; Andreazza, A; Andringa, S; Anjos, N; Antilogus, P; Apel, W D; Arnoud, Y; Ask, S; Åsman, B; Augustin, J E; Augustinus, A; Baillon, Paul; Ballestrero, A; Bambade, P; Barbier, R; Bardin, Dimitri Yuri; Barker, G; Baroncelli, A; Battaglia, Marco; Baubillier, M; Becks, K H; Begalli, M; Behrmann, A; Ben-Haim, E; Benekos, N C; Benvenuti, Alberto C; Bérat, C; Berggren, M; Berntzon, L; Bertrand, D; Besançon, M; Besson, N; Bloch, D; Blom, M; Bluj, M; Bonesini, M; Boonekamp, M; Booth, P S L; Borisov, G; Botner, O; Bouquet, B; Bowcock, T J V; Boyko, I; Bracko, M; Brenner, R; Brodet, E; Brückman, P; Brunet, J M; Bugge, L; Buschmann, P; Calvi, M; Camporesi, T; Canale, V; Carena, F; Castro, N; Cavallo, F R; Chapkin, M M; Charpentier, P; Checchia, P; Chierici, R; Shlyapnikov, P; Chudoba, J; Chung, S U; Cieslik, K; Collins, P; Contri, R; Cosme, G; Cossutti, F; Costa, M J; Crawley, B; Crennell, D J; Cuevas-Maestro, J; D'Hondt, J; Dalmau, J; Da Silva, T; Da Silva, W; Della Ricca, G; De Angelis, A; de Boer, Wim; De Clercq, C; De Lotto, B; De Maria, N; De Min, A; De Paula, L S; Di Ciaccio, Lucia; Di Simone, A; Doroba, K; Drees, J; Dris, M; Eigen, G; Ekelöf, T J C; Ellert, M; Elsing, M; Espirito-Santo, M C; Fanourakis, G K; Fassouliotis, D; Feindt, M; Fernández, J; Ferrer, A; Ferro, F; Flagmeyer, U; Föth, H; Fokitis, E; Fulda-Quenzer, F; Fuster, J A; Gandelman, M; García, C; Gavillet, P; Gazis, E N; Gokieli, R; Golob, B; Gómez-Ceballos, G; Gonçalves, P; Graziani, E; Grosdidier, G; Grzelak, K; Guy, J; Haag, C; Hallgren, A; Hamacher, K; Hamilton, K; Hansen, J; Haug, S; Hauler, F; Hedberg, V; Hennecke, M; Herr, H; Hoffman, J; Holmgren, S O; Holt, P J; Houlden, M A; Hultqvist, K; Jackson, J N; Jarlskog, G; Jarry, P; Jeans, D; Johansson, E K; Johansson, P D; Jonsson, P; Joram, C; Jungermann, L; Kapusta, F; Katsanevas, S; Katsoufis, E C; Kernel, G; Kersevan, Borut P; Kiiskinen, A P; King, B T; Kjaer, N J; Kluit, P; Kokkinias, P; Kourkoumelis, C; Kuznetsov, O; Krumshtein, Z; Kucharczyk, M; Lamsa, J; Leder, G; Ledroit, F; Leinonen, L; Leitner, R; Lemonne, J; Lepeltier, V; Lesiak, T; Liebig, W; Liko, D; Lipniacka, A; Lopes, J H; López, J M; Loukas, D; Lutz, P; Lyons, L; MacNaughton, J; Malek, A; Maltezos, S; Mandl, F; Marco, J; Marco, R; Maréchal, B; Margoni, M; Marin, J C; Mariotti, C; Markou, A; Martínez-Rivero, C; Masik, J; Mastroyiannopoulos, N; Matorras, F; Matteuzzi, C; Mazzucato, F; Mazzucato, M; McNulty, R; Meroni, C; Meyer, W T; Migliore, E; Mitaroff, W A; Mjörnmark, U; Moa, T; Moch, M; Mönig, K; Monge, R; Montenegro, J; Moraes, D; Moreno, S; Morettini, P; Müller, U; Münich, K; Mulders, M; Mundim, L M; Murray, W; Muryn, B; Myatt, Gerald; Myklebust, T; Nassiakou, M; Navarria, Francesco Luigi; Nawrocki, K; Nicolaidou, R; Nikolenko, M; Oblakowska-Mucha, A; Obraztsov, V F; Olshevskii, A G; Onofre, A; Orava, Risto; Österberg, K; Ouraou, A; Oyanguren, A; Paganoni, M; Paiano, S; Palacios, J P; Palka, H; Papadopoulou, T D; Pape, L; Parkes, C; Parodi, F; Parzefall, U; Passeri, A; Passon, O; Peralta, L; Perepelitsa, V F; Perrotta, A; Petrolini, A; Piedra, J; Pieri, L; Pierre, F; Pimenta, M; Piotto, E; Podobnik, T; Poireau, V; Pol, M E; Polok, G; Poropat, P; Pozdnyakov, V; Pukhaeva, N; Pullia, Antonio; Rames, J; Ramler, L; Read, A; Rebecchi, P; Rehn, J; Reid, D; Reinhardt, R; Renton, P B; Richard, F; Rídky, J; Rivero, M; Rodríguez, D; Romero, A; Ronchese, P; Rosenberg, E I; Roudeau, Patrick; Rovelli, T; Ruhlmann-Kleider, V; Ryabtchikov, D; Sadovskii, A; Salmi, L; Salt, J; Savoy-Navarro, A; Schwickerath, U; Segar, A; Sekulin, R L; Siebel, M; Sissakian, A N; Smadja, G; Smirnova, O G; Sokolov, A; Sopczak, A; Sosnowski, R; Spassoff, Tz; Stanitzki, M; Stocchi, A; Strauss, J; Stugu, B; Szczekowski, M; Szeptycka, M; Szumlak, T; Tabarelli de Fatis, T; Taffard, A C; Tegenfeldt, F; Timmermans, J; Tkatchev, L G; Tobin, M; Todorovova, S; Tomé, B; Tonazzo, A; Tortosa, P; Travnicek, P; Treille, D; Tristram, G; Trochimczuk, M; Troncon, C; Turluer, M L; Tyapkin, I A; Tyapkin, P; Tzamarias, S; Uvarov, V; Valenti, G; van Dam, P; Van Eldik, J; Van Lysebetten, A; Van Remortel, N; Van Vulpen, I B; Vegni, G; Veloso, F; Venus, W A; Verbeure, F; Verdier, P; Verzi, V; Vilanova, D; Vitale, L; Vrba, V; Wahlen, H; Washbrook, A J; Weiser, C; Wicke, D; Wickens, J H; Wilkinson, G; Winter, M; Witek, M; Yushchenko, O P; Zalewska-Bak, A; Zalewski, Piotr; Zavrtanik, D; Zhuravlov, V; Zimin, N I; Zinchenko, A I; Zupan, M

    2003-01-01

    DELPHI results are presented on the inclusive production of two (K Kbar pi)^0 states in the mass region 1.2-1.6 GeV/c^2 in hadronic Z decays at LEP I. The measured masses (widths) are 1274 +/- 6 MeV/c^2 (29 +/- 12 MeV/c^2) and 1426 +/- 6 MeV/c^2 (51 +/- 14 MeV/c^2) respectively. A partial-wave analysis of the (K Kbar pi)^0 system shows that the first peak is consistent with the I^G(J^{PC})=0^+(1^{++})/(0^{-+}) a_0(980)pi and the second with the I^G(J^{PC})=0^+(1^{++}) K^*(892)Kbar + c.c. assignments. The total hadronic production rates per hadronic Z decay are (0.165 +/- 0.051) and (0.056 +/- 0.012) respectively. These measurements are consistent with the two states being the f_1(1285) and f_1(1420) mesons.

  9. Uji Resistensi Progeni F1 HP 1998 Terhadap Penyakit Gugur Daun Colletotrichum gloeoesporioides (Penz). Sacc Pada Tanaman Karet (Hevea brasiliensis Muell Arg.) di Laboratorium

    OpenAIRE

    Purnomo, Ary

    2011-01-01

    The research was conducted in Laboratory Plant Protection Sungei Putih Rubber Research Center since November 2009 to December 2009. The aims of the research was to know level of resitance of rubber F1 HP 1998 cultivar to fall of leaf C. gloeoesporioides disease. The research used the desigen Complete Random Device (CRD) non factorial with 31 treatmens ( 30 F1 HP 1998 cultivar and 1 control clone) and 3 mutliplication. The rubber F1 HP 1998 cultivar were used is PN No...

  10. Mortality of western corn rootworm larvae on MIR604 transgenic maize roots: field survivorship has no significant impact on survivorship of F1 progeny on MIR604.

    Science.gov (United States)

    Hibbard, Bruce E; Clark, Thomas L; Ellersieck, Mark R; Meihls, Lisa N; El Khishen, Ahmed A; Kaster, Von; Steiner, Henry-York; Kurtz, Ryan

    2010-12-01

    Mortality of western corn rootworm, Diabrotica virgifera virgifera LeConte, larvae due to MIR604 transgenic corn, Zea mays L., expressing the modified Cry3A (mCry3A) protein relative to survivorship on corn with the same genetic background without the gene (isoline corn) was evaluated at three Missouri sites in both 2005 and 2006. We made these comparisons by using wild-type western corn rootworm at three different egg densities (6,000, 3,000, and 1,500 eggs per m) so that the role of density-dependent mortality would be known. The mortality due to the mCry3A protein was 94.88% when averaged across all environments and both years. Fifty percent emergence of beetles was delayed approximately 5.5 d. Beetles were kept alive and their progeny evaluated on MIR604 and isoline corn in the greenhouse to determine whether survivorship on MIR604 in the field for one generation increased survivorship on MIR604 in the greenhouse in the subsequent generation. There was no significant difference in survivorship on MIR604 in greenhouse assays between larvae whose parents survived isoline and larvae whose parents survived MIR604 in the field the previous generation, indicating that many susceptible beetles survived MIR604 in the field the previous season along with any potentially resistant beetles. The data are discussed in terms of rootworm insect resistance management.

  11. In Utero Nutritional Manipulation Provokes Dysregulated Adipocytokines Production in F1 Offspring in Rats

    Directory of Open Access Journals (Sweden)

    Mervat Y. Hanafi

    2016-01-01

    Full Text Available Background. Intrauterine environment plays a pivotal role in the origin of fatal diseases such as diabetes. Diabetes and obesity are associated with low-grade inflammatory state and dysregulated adipokines production. This study aims to investigate the effect of maternal obesity and malnutrition on adipokines production (adiponectin, leptin, and TNF-α in F1 offspring in rats. Materials and Methods. Wistar rats were allocated in groups: F1 offspring of control mothers under control diet (CF1-CD and under high-fat diet (CF1-HCD, F1 offspring of obese mothers under CD (OF1-CD and under HCD (OF1-HCD, and F1 offspring of malnourished mothers under CD (MF1-CD and under HCD (MF1-HCD. Every 5 weeks postnatally, blood samples were obtained for biochemical analysis. Results. At the end of the 30-week follow-up, OF1-HCD and MF1-HCD exhibited hyperinsulinemia, moderate dyslipidemia, insulin resistance, and impaired glucose homeostasis compared to CF1-CD and CF1-HCD. OF1-HCD and MF1-HCD demonstrated low serum levels of adiponectin and high levels of leptin compared to CF1-CD and CF1-HCD. OF1-CD, OF1-HCD, and MF1-HCD had elevated serum levels of TNF-α compared to CF1-CD and CF1-HCD (p<0.05. Conclusion. Maternal nutritional manipulation predisposes the offspring to development of insulin resistance in their adult life, probably via instigating dysregulated adipokines production.

  12. Less is more: reduced catechol production permits Pseudomonas putida F1 to grow on styrene.

    Science.gov (United States)

    George, Kevin W; Hay, Anthony

    2012-11-01

    Pseudomonas putida F1 is unable to grow on styrene due to the accumulation of 3-vinylcatechol, a toxic metabolite that is produced through the toluene degradation (tod) pathway and causes catechol-2,3-dioxygenase (C23O) inactivation. In this study, we characterized a spontaneous F1 mutant, designated SF1, which acquired the ability to grow on styrene and did not accumulate 3-vinylcatechol. Whereas adaptation to new aromatic substrates has typically been shown to involve increased C23O activity or the acquisition of resistance to C23O inactivation, SF1 retained wild-type C23O activity. Surprisingly, SF1 grew more slowly on toluene, its native substrate, and exhibited reduced toluene dioxygenase (TDO) activity (approximately 50 % of that of F1), the enzyme responsible for ring hydroxylation and subsequent production of 3-vinylcatechol. DNA sequence analysis of the tod operon of SF1 revealed a single base pair mutation in todA (C479T), a gene encoding the reductase component of TDO. Replacement of the wild-type todA allele in F1 with todA(C479T) reduced TDO activity to SF1 levels, obviated vinylcatechol accumulation, and conferred the ability to grow on styrene. This novel 'less is more' strategy - reduced catechol production as a means to expand growth substrate range - sheds light on an alternative approach for managing catechol toxicity during the metabolism of aromatic compounds.

  13. Measurement of inclusive f$_{1}$(1285) and f$_{1}$(1420) production in Z decays with the DELPHI detector

    CERN Document Server

    Abdallah, J; Adam, W; Adzic, P; Albrecht, T; Alderweireld, T; Alemany-Fernandez, R; Allmendinger, T; Allport, P P; Amaldi, Ugo; Amapane, N; Amato, S; Anashkin, E; Andreazza, A; Andringa, S; Anjos, N; Antilogus, P; Apel, W D; Arnoud, Y; Ask, S; Åsman, B; Augustin, J E; Augustinus, A; Baillon, Paul; Ballestrero, A; Bambade, P; Barbier, R; Bardin, D; Barker, G; Baroncelli, A; Battaglia, M; Baubillier, M; Becks, K H; Begalli, M; Behrmann, A; Ben-Haim, E; Benekos, N; Benvenuti, A C; Bérat, C; Berggren, M; Berntzon, L; Bertrand, D; Besançon, M; Besson, N; Bloch, D; Blom, M; Bluj, M; Bonesini, M; Boonekamp, M; Booth, P S L; Borisov, G; Botner, O; Bouquet, B; Bowcock, T J V; Boyko, I; Bracko, M; Brenner, R; Brodet, E; Brückman, P; Brunet, J M; Bugge, L; Buschmann, P; Calvi, M; Camporesi, T; Canale, V; Carena, F; Castro, N; Cavallo, F; Chapkin, M; Charpentier, P; Checchia, P; Chierici, R; Chliapnikov, P V; Chudoba, J; Chung, S U; Cieslik, K; Collins, P; Contri, R; Cosme, G; Cossutti, F; Costa, M J; Crawley, B; Crennell, D J; Cuevas-Maestro, J; D'Hondt, J; Da Silva, W; Dalmau, J; De Angelis, A; de Boer, Wim; De Clercq, C; De Lotto, B; De Maria, N; De Min, A; Della Ricca, G; Di Ciaccio, L; Di Simone, A; Doroba, K; Drees, J; Dris, M; Eigen, G; Ekelöf, T J C; Ellert, M; Elsing, M; Fanourakis, G K; Fassouliotis, D; Feindt, M; Fernández, J; Ferrer, A; Ferro, F; Flagmeyer, U; Föth, H; Fokitis, E; Fulda-Quenzer, F; Fuster, J; Gandleman, M; García, C; Gavillet, P; Gazis, E; Gokieli, R; Golob, B; Gómez-Ceballos, G; Gonçalves, P; Graziani, E; Grosdidier, G; Grzelak, K; Guy, J; Haag, C; Hallgren, A; Hamacher, K; Hamilton, K; Hansen, J; Haug, S; Hauler, F; Hedberg, V; Hennecke, M; Herr, H; Hoffman, J; Holmgren, S O; Holt, P J; Houlden, M A; Hultqvist, K; Jackson, J N; Jarlskog, G; Jarry, P; Jeans, D; Johansson, E K; Johansson, P D; Jonsson, P; Joram, C; Jungermann, L; Kapusta, F; Katsanevas, S; Katsoufis, E C; Kernel, G; Kersevan, B P; Kiiskinen, A P; King, B T; Kjaer, N J; Kluit, P; Kokkinias, P; Kourkoumelis, C; Kuznetsov, O; Krumshtein, Z; Kucharczyk, M; Lamsa, J; Leder, G; Ledroit, F; Leinonen, L; Leitner, R; Lemonne, J; Lepeltier, V; Lesiak, T; Liebig, W; Liko, D; Lipniacka, A; Lopes, J H; López, J M; Loukas, D; Lutz, P; Lyons, L; MacNaughton, J; Malek, A; Maltezos, S; Mandl, F; Marco, J; Marco, R; Maréchal, B; Margoni, M; Marin, J C; Mariotti, C; Markou, A; Martínez-Rivero, C; Masik, J; Mastroyiannopoulos, N; Matorras, F; Matteuzzi, C; Mazzucato, F; Mazzucato, M; McNulty, R; Meroni, C; Meyer, W T; Migliore, E; Mitaroff, W A; Mjörnmark, U; Moa, T; Moch, M; Mönig, K; Monge, R; Montenegro, J; Moraes, D; Moreno, S; Morettini, P; Müller, U; Münich, K; Mulders, M; Mundim, L; Murray, W; Muryn, B; Myatt, G; Myklebust, T; Nassiakou, M; Navarria, Francesco Luigi; Nawrocki, K; Nicolaidou, R; Nikolenko, M; Oblakowska-Mucha, A; Obraztsov, V F; Olshevskii, A G; Onofre, A; Orava, R; Österberg, K; Ouraou, A; Oyanguren, A; Paganoni, M; Paiano, S; Palacios, J P; Palka, H; Papadopoulou, T D; Pape, L; Parkes, C; Parodi, F; Parzefall, U; Passeri, A; Passon, O; Peralta, L; Perepelitsa, V F; Perrotta, A; Petrolini, A; Piedra, J; Pieri, L; Pierre, F; Pimenta, M; Piotto, E; Podobnik, T; Poireau, V; Pol, M E; Polok, G; Poropat, P; Pozdnyakov, V; Pukhaeva, N; Pullia, A; Rames, J; Ramler, L; Read, A; Rebecchi, P; Rehn, J; Reid, D; Reinhardt, R; Renton, P B; Richard, F; Rídky, J; Rivero, M; Rodríguez, D; Romero, A; Ronchese, P; Rosenberg, E I; Roudeau, P; Rovelli, T; Ruhlmann-Kleider, V; Ryabtchikov, D; Sadovskii, A; Salmi, L; Salt, J; Espirito-Santo, M C; Savoy-Navarro, A; Schwickerath, U; Segar, A; Sekulin, R L; Siebel, M; Sisakian, A; Smadja, G; Smirnova, O; Sokolov, A; Sopczak, A; Sosnowski, R; Spassoff, Tz; Stanitzki, M; Stocchi, A; Strauss, J; Stugu, B; Szczekowski, M; Szeptycka, M; Szumlak, T; Tabarelli de Fatis, T; Taffard, A C; Tegenfeldt, F; Timmermans, J; Tkatchev, L G; Tobin, M; Todorovova, S; Tomé, B; Tonazzo, A; Tortosa, P; Travnicek, P; Treille, D; Tristram, G; Trochimczuk, M; Troncon, C; Turluer, M L; Tyapkin, I A; Tyapkin, P; Tzamarias, S; Uvarov, V; Valenti, G; van Dam, P; Van Eldik, J; Van Lysebetten, A; Van Vulpen, I; Vegni, G; Veloso, F; Venus, W; Verbeure, F; Verdier, P; Verzi, V; Vilanova, D; Vitale, L; Vrba, V; Wahlen, H; Washbrook, A J; Weiser, C; Wicke, D; Wickens, J; Wilkinson, G; Winter, M; Witek, M; Yushchenko, O P; Zalewska-Bak, A; Zalewski, P; Zavrtanik, D; Zhuravlov, V; Zimin, N I; Zintchenko, A; Zupan, M; Da Silva, T; De Paula, L; Van Remortel, N

    2003-01-01

    DELPHI results are presented on the inclusive production of two (KK pi )/sup 0/ states in the mass region 1.2-1.6 GeV/c/sup 2/ in hadronic Z decays at LEP I. The measured masses (widths) are 1274+or-6 MeV/c/sup 2/ (29+or-12 MeV/c/sup 2/) and 1426+or-6 MeV/c /sup 2/ (51+or-14 MeV/c/sup 2/), respectively. A partial-wave analysis of the (KK pi )/sup 0/ system shows that the first peak is consistent with the I/sup G/(J/sup PC/)=0/sup +/(1/sup ++/)/(0/sup -+ /)a/sub 0/(980) pi and the second with the I/sup G/(J/sup PC/)=0/sup +/(1/sup ++/)K/sup */(892)K+c.c. assignments. The total hadronic production rates per hadronic Z decay are (0.165+or-0.051) and (0.056+or-0.012), respectively. These measurements are consistent with the two states being the f/sub 1/(1285) and f/sub 1/(1420) mesons.

  14. COMPARATIVE PRODUCTIVE PERFORMANCE OF DESI PARENT CHICKENS AND THEIR FIRST PROGENY MAINTAINED ON DEEP LITTER SYSTEM

    Directory of Open Access Journals (Sweden)

    A.W. Sahota, B. M. Bhatti and L. A. Akhtar

    2003-01-01

    Full Text Available This study was undertaken to examine effect of selection process of Desi field chickens on the productive performance of Desi progeny versus their parents maintained under deep litter system. For this purpose, 300 adult pullets (about 30 weeks-old each of Desi parents and their first progeny were maintained in 4 separate pens on floor under optimal managemental conditions for experimental period of one year. The experimental birds were fed ad-libitum a balanced layer’s ration prepared at Nutrition Section of this Institute. They had free access to clean and fresh drinking water. 16 hours lighting schedule giving natural light supplemented with artificial light was followed. The results indicated significant (p<0.01 improvement in egg production, feed conversion efficiency and egg mass of Desi progeny in comparison to their parents which could be attributed to effect of breeding using selection process carried out in Desi parent. However, performance of Desi progeny in respect of egg size and mortality rate varied non-significantly from their parents. Based on the findings of this study it may be concluded that productive performance of Desi native chickens could be improved through breeding using selection technique.

  15. Mapping Quantitative Trait Loci Controlling Milk Production in Dairy Cattle by Exploiting Progeny Testing

    Science.gov (United States)

    Georges, M.; Nielsen, D.; Mackinnon, M.; Mishra, A.; Okimoto, R.; Pasquino, A. T.; Sargeant, L. S.; Sorensen, A.; Steele, M. R.; Zhao, X.; Womack, J. E.; Hoeschele, I.

    1995-01-01

    We have exploited ``progeny testing'' to map quantitative trait loci (QTL) underlying the genetic variation of milk production in a selected dairy cattle population. A total of 1,518 sires, with progeny tests based on the milking performances of > 150,000 daughters jointly, was genotyped for 159 autosomal microsatellites bracketing 1645 centimorgan or approximately two thirds of the bovine genome. Using a maximum likelihood multilocus linkage analysis accounting for variance heterogeneity of the phenotypes, we identified five chromosomes giving very strong evidence (LOD score >/= 3) for the presence of a QTL controlling milk production: chromosomes 1, 6, 9, 10 and 20. These findings demonstrate that loci with considerable effects on milk production are still segregating in highly selected populations and pave the way toward marker-assisted selection in dairy cattle breeding. PMID:7713441

  16. Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing

    Energy Technology Data Exchange (ETDEWEB)

    Georges, M.; Nielsen, D.; Mackinnon, M.; Mishra, A.; Okimoto, R.; Sargeant, L.S.; Steele, M.R.; Zhao, X. [Genmark Inc., Salt Lake City, UT (United States); Pasquino, A.T. [Virginia Polytechnic Institute and State Univ., Blacksburg, VA (United States)

    1995-02-01

    We have exploited {open_quotes}progeny testing{close_quotes} to map quantitative trait loci (QTL) underlying the genetic variation of milk production in a selected dairy cattle population. A total of 1,518 sires, with progeny tests based on the milking performances of >150,000 daughters jointly, was genotyped for 159 autosomal microsatellites bracketing 1645 centimorgan or approximately two thirds of the bovine genome. Using a maximum likelihood multilocus linkage analysis accounting for variance heterogeneity of the phenotypes, we identified five chromosomes giving very strong evidence (LOD score {ge} 3) for the presence of a QTL controlling milk production: chromosomes 1, 6, 9, 10 and 20. These findings demonstrate that loci with considerable effects on milk production are still segregating in highly selected populations and pave the way toward marker-assisted selection in dairy cattle breeding. 44 refs., 4 figs., 3 tabs.

  17. ROS production is essential for the apoptotic function of E2F1 in pheochromocytoma and neuroblastoma cell lines.

    Directory of Open Access Journals (Sweden)

    Lilia Espada

    Full Text Available In this study we demonstrate that accumulation of reactive oxygen species (ROS is essential for E2F1 mediated apoptosis in ER-E2F1 PC12 pheochromocytoma, and SH-SY5Y and SK-N-JD neuroblastoma stable cell lines. In these cells, the ER-E2F1 fusion protein is expressed in the cytosol; the addition of 4-hydroxytamoxifen (OHT induces its translocation to the nucleus and activation of E2F1target genes. Previously we demonstrated that, in ER-E2F1 PC12 cells, OHT treatment induced apoptosis through activation of caspase-3. Here we show that caspase-8 activity did not change upon treatment with OHT. Moreover, over-expression of Bcl-xL arrested OHT-induced apoptosis; by contrast, over-expression of c-FLIP, did not have any effect on OHT-induced apoptosis. OHT addition induces BimL expression, its translocation to mitochondria and activation of Bax, which is paralleled by diminished mitochondrial enrichment of Bcl-xL. Treatment with a Bax-inhibitory peptide reduced OHT-induced apoptosis. These results point out the essential role of mitochondria on the apoptotic process driven by E2F1. ROS accumulation followed E2F1 induction and treatment with the antioxidant N-acetylcysteine, inhibited E2F1-induced Bax translocation to mitochondria and subsequent apoptosis. The role of ROS in mediating OHT-induced apoptosis was also studied in two neuroblastoma cell lines, SH-SY5Y and SK-N-JD. In SH-SY5Y cells, activation of E2F1 by the addition of OHT induced ROS production and apoptosis, whereas over-expression of E2F1 in SK-N-JD cells failed to induce either response. Transcriptional profiling revealed that many of the genes responsible for scavenging ROS were down-regulated following E2F1-induction in SH-SY5Y, but not in SK-N-JD cells. Finally, inhibition of GSK3β blocked ROS production, Bax activation and the down regulation of ROS scavenging genes. These findings provide an explanation for the apparent contradictory role of E2F1 as an apoptotic agent versus a cell

  18. Sex determination in beetles: Production of all male progeny by Parental RNAi knockdown of transformer

    Science.gov (United States)

    Shukla, Jayendra Nath; Palli, Subba Reddy

    2012-01-01

    Sex in insects is determined by a cascade of regulators ultimately controlling sex-specific splicing of a transcription factor, Doublesex (Dsx). We recently identified homolog of dsx in the red flour beetle, Tribolium castaneum (Tcdsx). Here, we report on the identification and characterization of a regulator of Tcdsx splicing in T. castaneum. Two male-specific and one female-specific isoforms of T. castaneum transformer (Tctra) were identified. RNA interference-aided knockdown of Tctra in pupa or adults caused a change in sex from females to males by diverting the splicing of Tcdsx pre-mRNA to male-specific isoform. All the pupa and adults developed from Tctra dsRNA injected final instar larvae showed male-specific sexually dimorphic structures. Tctra parental RNAi caused an elimination of females from the progeny resulting in production of all male progeny. Transformer parental RNAi could be used to produce all male population for use in pest control though sterile male release methods. PMID:22924109

  19. Expression of heterosis for productive traits in F1 eggplant (Solanum melongena L. hybrids

    Directory of Open Access Journals (Sweden)

    João Alencar de Sousa

    1998-03-01

    Full Text Available This study was carried out to obtain estimates of heterosis in crosses between seven eggplant cultivars (Embu = E; Santa Genebra = SG; Viserba = V; Aubergine de Barbentane = AB; Florida Market 10 = FM; Black Beauty = BB, and Melitino = M and two breeding lines (B-14-07 = B1 and B-31-06 = B2. The F1 hybrids used were: E x FM; E x BB; E x M; E x B1; E x B2; SG x FM; SG x BB; SG x M; SG x B1; SG x B2; V x FM; V x B1; V x B2; AB x FM; AB x M; AB x B1; AB x B2 and M x FM. Cultivars, lines and hybrids were evaluated at the ESAL experimental field in Lavras, MG, from February to October 1992. A randomized complete block design with three replications was used. Significant heterosis relative to the parental means was detected for all traits studied. Their values ranged from +41.23% to +113.31% for total fruit yield, from -11.45% to +26.17% for average fruit weight, and from +27.98% to +141.81% for early production. Heterosis relative to the superior parent ranged from +13.89% to +92.51% for total fruit yield. Hybrid pairs: SG x FM and AB x B1, V x FM and AB x FM, E x M and AB x B1 were the most heterotic relative to the parental mean for total fruit production, mean fruit weight and early production, respectively. The hybrids displaying highest heterosis relative to the superior parent for total yield were AB x B1 and SG x FM.Este trabalho visou obter estimativas da heterose em cruzamentos entre sete cultivares de berinjela (Embu = E, Santa Genebra = SG, Viserba = V, Aubergine de Barbentane = AB, Florida Market 10 = FM, Black Beauty = BB e Melitino = M e duas linhagens (B-14-07 = B1 e B-31-06 = B2. Os híbridos F1 utilizados foram: E x FM; E x BB; E x M; E x B1; E x B2; SG x FM; SG x BB; SG x M; SG x B1; SG x B2; V x FM; V x B1; V x B2; AB x FM; AB x M; AB x B1; AB x B2 e M x FM. O trabalho foi conduzido no campus da ESAL em Lavras-MG, de fevereiro a outubro de 1992. O delineamento empregado foi o de blocos casualizados completos com três repeti

  20. Effects on milk production in F1 crossbred of Alpine goat breed (♂ and Albanian goat breed (♀

    Directory of Open Access Journals (Sweden)

    Luan Hajno

    2012-07-01

    Full Text Available About 950,000 goats, farmed mostly in hilly and mountainous areas of Albania, contribute about 8% of the country’s total milk production. In order to increase milk production, farmers are currently using crosses of the local goat breed with exotic breeds, mainly the Alpine breed from France. This study examines milk production data of first lactation from 45 goats of the local breed, 82 goats of the Alpine breed and 58 F1 crosses (♂Alpine breed x ♀local breed. The goats were kept on small-scale farms according to the traditional Albanian system. Milking was carried out in the morning and evening. Kids were weaned at 65 days of age after which milking started. Milk yield was recorded twice with a 15-day interval between the two readings. Total milk yield was calculated using the Fleischmann method. The F1 goats produced 37.8 kg more milk than local breed goats although the lactation length (P<0.05 of F1 goats was six days shorter compared to that of local breed goats (P<0.05. Analysis of variance showed a highly significant effect (P<0.01 of the genotype factor on milk production. The average Cappio-Borlino curves of three genotypes indicated that the lactation curves of local breed and F1 crosses were similar. Although the F1 cross goats had 50% of their genomes from a genetically improved breed they were still able to deal with the difficult conditions that characterize the traditional extensive farming systems in Albania. Breeding pure Alpine breed or its crosses with the local goat breed improved milk production in an extensive traditional system.

  1. Psychoacoustical and ear canal cancellation of (2f1-f2)-distortion products.

    Science.gov (United States)

    Zwicker, E; Harris, F P

    1990-06-01

    Level and phase of the (2f1-f2)-difference tone were measured as a function of primary-tone level using the psychoacoustical method of cancellation and the objective method of emission cancellation for four frequency separations of f1 = 1620 Hz and f2 in four subjects. Differences between hearing- and emission-cancellation levels ranged from 60-33 dB as delta f = f2-f1 increased from 180 to 432 Hz. For smaller separations of the primaries, phase changes for emission cancellation covered a wide range and had sharp "steps," whereas for hearing cancellation, the phase varied only slightly. With wider separations of the primaries, the phase became more varied for hearing cancellation and more homogeneous for emission cancellation. Both emission- and hearing-cancellation level functions were nonmonotonic as a function of constant SL1 and varied SL2. Remarkable phase shifts always appeared near minima in level at all separations of the primaries for emission cancellation. Four sources may be contributing to the differences in results: (a) the frequency-dependent attenuation of the middle-ear transfer function, (b) the frequency-dependent mismatch of the acoustical impedances at the eardrum, (c) the frequency dependence of the microphone's sensitivity mounted within the probe, and (d) the different reaction of active nonlinear cochlear processes on the hearing- and emission-cancellation tones.

  2. On the Hybrid F1 Characteristics of Physiology, Biochemistry, Product Quality and Resistance to Black Shank in Oriental Tobacco

    Directory of Open Access Journals (Sweden)

    Chen X

    2014-12-01

    Full Text Available Experiments were conducted from 1996 to 1998 at the Hefei Institute of Economics and Technology and at the Oriental Experimental Station of the Zhejiang Province (China. Seven F1 hybrids and three parental varieties of Oriental tobaccos were evaluated for the characteristics of photosynthetic and transpiration rates, esterase isozymes, resistance to black shank, quality and product potential from the 1996-1998 growing seasons. Tobacco leaves had higher photosynthetic rates and many differences among genotypes in the early stage of plant vigorous growth compared with more mature leaves. However, transpiration rates were lower in the younger leaves and greater in the more mature leaves. All the entries had four common bands (B1, B3, B4 and B6 of the esterase isoenzymes. Differences between entries resulted from in having or not having the B2 and B5 bands and color intensity differences of all the bands. These differences could be used to identify individual entries. The F1hybrids Samsun X Toy and Samsun X Argjiro, compared with the CK Samsun control, had obvious heterotic vigor in the characteristics of product, for yield, quality and resistance to black shank. The F1 hybrid Samsun X Toy maintained higher photosynthetic and transpiration rates in the two growth stages compared to other entries. However, the F1hybrid Samsun X Argjiro had higher photosynthetic rates and lower transpiration rates in the early growth stage and the two rates were lower in the later stage, but it maintained higher photosynthetic rates for the whole growth stage. Net photosynthetic rates had a significant positive correlation with yield product, quality and resistance to black shank of the Oriental tobacco F1hybrids.

  3. Data on melanin production in B16F1 melanoma cells in the presence of emu oil

    Directory of Open Access Journals (Sweden)

    Minoru Ito

    2016-12-01

    Full Text Available Here, we present data on the effects of emu oil, obtained from emu (Dromaius novaehollandiae fat deposits, on melanogenesis in B16F1 murine melanoma cells. The cells were cultured in media containing different concentrations of emu oil, and the melanin content of these cells was measured using a microplate reader. Next, melanin content was measured for cells cultured with α-melanocyte-stimulating hormone. This article reports the different melanin contents as μg melanin/mg cellular protein, by using bar graphs with error bars. The present data imply that emu oil reduces the cellular melanin production.

  4. Estimating the fraction of progeny virions that must incorporate APOBEC3G for suppression of productive HIV-1 infection

    Energy Technology Data Exchange (ETDEWEB)

    Thangavelu, Pulari U.; Gupta, Vipul; Dixit, Narendra M., E-mail: narendra@chemeng.iisc.ernet.in

    2014-01-20

    The contest between the host factor APOBEC3G (A3G) and the HIV-1 protein Vif presents an attractive target of intervention. The extent to which the A3G–Vif interaction must be suppressed to tilt the balance in favor of A3G remains unknown. We employed stochastic simulations and mathematical modeling of the within-host dynamics and evolution of HIV-1 to estimate the fraction of progeny virions that must incorporate A3G to render productive infection unsustainable. Using three different approaches, we found consistently that a transition from sustained infection to suppression of productive infection occurred when the latter fraction exceeded ∼0.8. The transition was triggered by A3G-induced hypermutations that led to premature stop codons compromising viral production and was consistent with driving the basic reproductive number, R{sub 0}, below unity. The fraction identified may serve as a quantitative guideline for strategies targeting the A3G–Vif axis. - Highlights: • We perform simulations and mathematical modeling of the role of APOBEC3G in suppressing HIV-1 infection. • In three distinct ways, we estimate that when over 80% of progeny virions carry APOBEC3G, productive HIV-1 infection would be suppressed. • Our estimate of this critical fraction presents quantitative guidelines for strategies targeting the APOBEC3G–Vif axis.

  5. Expression of genes encoding F-1-ATPase results in uncoupling of glycolysis from biomass production in Lactococcus lactis

    DEFF Research Database (Denmark)

    Købmann, Brian Jensen; Solem, Christian; Pedersen, M.B.

    2002-01-01

    We studied how the introduction of an additional ATP-consuming reaction affects the metabolic fluxes in Lactococcus lactis. Genes encoding the hydrolytic part of the F-1 domain of the membrane-bound (F1F0) H+-ATPase were expressed from a range of synthetic constitutive promoters. Expression...

  6. Correlation and Principal Component Analysis on Fruit Characters of Malus sieversii f.neidzwetzkyana F1 Progeny%新疆野苹果F1代果实性状的相关分析和主成分分析

    Institute of Scientific and Technical Information of China (English)

    刘大亮; 王进; 张艳敏; 宋君; 高利平; 陈学森

    2013-01-01

    以4年生新疆野苹果×富士杂交F1代群体为试材,对19个果实品质指标进行测定,利用相关分析和主成分分析从功能基因研究和品系选育两个角度对各指标的遗传变异进行考察.结果表明:(1)与栽培苹果相比,F1代在果实质地、花色苷有关性状之间的相关性存在差异:钙与果实质地的3个指标相关性不显著;花色苷与糖含量相关性不显著,但与钙、钾含量相关性极显著.钙与多个指标极显著相关,可作为功能基因研究的重点.(2)从主成分贡献率看,果实质地、矿质元素、果实大小、花色苷含量4个主成分在遗传信息中占的比重较大,是下一步功能基因研究的切入点,也是进行品质综合评价的重点考察对象.加大对果实质地、果实大小、花色苷含量的考察可提高育种效率.(3)对主成分综合得分排序,根据不同育种目标选择综合性状优良的个体,是新疆野苹果在育种中利用保存的有效捷径.%Using 4-year-old F1 hybrid population of Malus sieversii f.neidzwetzkyana and Fuji as materials,nineteen fruit quality indexes were measured to analyze genetic variations from two aespects,functional gene research and strain breeding,by correlation and principal component analysis.The results were as follows.Firstly,compared with cultivated apple,there were differences in correlation of fruit quality and anthocyanin in F1 hybrid:Ca had no significant correlation with 3 fruit quality indexes; anthocyanin had no significant correlation with sugar content,but highly significant correlations with Ca and K content.Because Ca had highly significant correlations with many indexes,it could be as the emphasis of functional gene research.Secondly,according to contribution rates of main compositions,fruit quality,mineral element,fruit size and anthocyanin content took larger proportion in genetic information.They would be the pointcuts in further research of functional genes and the key

  7. A Truncated Nef Peptide from SIVcpz Inhibits the Production of HIV-1 Infectious Progeny

    Directory of Open Access Journals (Sweden)

    Marcela Sabino Cunha

    2016-07-01

    Full Text Available Nef proteins from all primate Lentiviruses, including the simian immunodeficiency virus of chimpanzees (SIVcpz, increase viral progeny infectivity. However, the function of Nef involved with the increase in viral infectivity is still not completely understood. Nonetheless, until now, studies investigating the functions of Nef from SIVcpz have been conducted in the context of the HIV-1 proviruses. In an attempt to investigate the role played by Nef during the replication cycle of an SIVcpz, a Nef-defective derivative was obtained from the SIVcpzWTGab2 clone by introducing a frame shift mutation at a unique restriction site within the nef sequence. This nef-deleted clone expresses an N-terminal 74-amino acid truncated peptide of Nef and was named SIVcpz-tNef. We found that the SIVcpz-tNef does not behave as a classic nef-deleted HIV-1 or simian immunodeficiency virus of macaques SIVmac. Markedly, SIVcpz-tNef progeny from both Hek-293T and Molt producer cells were completely non-infectious. Moreover, the loss in infectivity of SIVcpz-tNef correlated with the inhibition of Gag and GagPol processing. A marked accumulation of Gag and very low levels of reverse transcriptase were detected in viral lysates. Furthermore, these observations were reproduced once the tNef peptide was expressed in trans both in SIVcpzΔNef and HIV-1WT expressing cells, demonstrating that the truncated peptide is a dominant negative for viral processing and infectivity for both SIVcpz and HIV-1. We demonstrated that the truncated Nef peptide binds to GagPol outside the protease region and by doing so probably blocks processing of both GagPol and Gag precursors at a very early stage. This study demonstrates for the first time that naturally-occurring Nef peptides can potently block lentiviral processing and infectivity.

  8. A Truncated Nef Peptide from SIVcpz Inhibits the Production of HIV-1 Infectious Progeny

    Science.gov (United States)

    Sabino Cunha, Marcela; Lima Sampaio, Thatiane; Peterlin, B. Matija; Jesus da Costa, Luciana

    2016-01-01

    Nef proteins from all primate Lentiviruses, including the simian immunodeficiency virus of chimpanzees (SIVcpz), increase viral progeny infectivity. However, the function of Nef involved with the increase in viral infectivity is still not completely understood. Nonetheless, until now, studies investigating the functions of Nef from SIVcpz have been conducted in the context of the HIV-1 proviruses. In an attempt to investigate the role played by Nef during the replication cycle of an SIVcpz, a Nef-defective derivative was obtained from the SIVcpzWTGab2 clone by introducing a frame shift mutation at a unique restriction site within the nef sequence. This nef-deleted clone expresses an N-terminal 74-amino acid truncated peptide of Nef and was named SIVcpz-tNef. We found that the SIVcpz-tNef does not behave as a classic nef-deleted HIV-1 or simian immunodeficiency virus of macaques SIVmac. Markedly, SIVcpz-tNef progeny from both Hek-293T and Molt producer cells were completely non-infectious. Moreover, the loss in infectivity of SIVcpz-tNef correlated with the inhibition of Gag and GagPol processing. A marked accumulation of Gag and very low levels of reverse transcriptase were detected in viral lysates. Furthermore, these observations were reproduced once the tNef peptide was expressed in trans both in SIVcpzΔNef and HIV-1WT expressing cells, demonstrating that the truncated peptide is a dominant negative for viral processing and infectivity for both SIVcpz and HIV-1. We demonstrated that the truncated Nef peptide binds to GagPol outside the protease region and by doing so probably blocks processing of both GagPol and Gag precursors at a very early stage. This study demonstrates for the first time that naturally-occurring Nef peptides can potently block lentiviral processing and infectivity. PMID:27399760

  9. Ipsilateral distortion product otoacoustic emission (2f1-f2) suppression in children with sensorineural hearing loss.

    Science.gov (United States)

    Abdala, Carolina; Fitzgerald, Tracy S

    2003-08-01

    Distortion product otoacoustic emission (DPOAE) ipsilateral suppression has been applied to study cochlear function and maturation in laboratory animals and humans. Although DPOAE suppression appears to be sensitive to regions of specialized cochlear function and to cochlear immaturity, it is not known whether it reflects permanent cochlear damage, i.e., sensorineural hearing loss (SNHL), in a reliable and systematic manner in humans. Eight school-aged children with mild-moderate SNHL and 20 normal-hearing children served as subjects in this study. DPOAE (2f1-f2) suppression data were collected at four f2 frequencies (1500, 3000, 4000, and 6000 Hz) using moderate-level primary tones. Features of the DPOAE iso-suppression tuning curves and suppression growth were analyzed for both subject groups. Results show that DPOAE suppression tuning curves from hearing-impaired subjects can be reliably recorded. DPOAE suppression tuning curves were generally normal in appearance and shape for six out of eight hearing-impaired subjects but showed subtle abnormalities in at least one feature. There was not one single trend or pattern of abnormality that characterized all hearing-impaired subjects. The most prominent patterns of abnormality included: broadened tuning, elevated tip, and downward shift of tip frequency. The unique patterns of atypical DPOAE suppression in subjects with similar audiograms may suggest different patterns of underlying sensory cell damage. This speculation warrants further investigation.

  10. Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle

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    Sonia Zakizadeh

    2015-04-01

    Full Text Available Specific trait candidate genes are sequenced genes with known biological activity. The effects of POU1F1 and STAT5A on milk production traits have been studied in several studies. POU1F1 affects on transcription of prolactin and growth hormone gene, as well as, STAT5A is known as a main mediator of growth hormone action on target genes and intracellular mediator of prolactin signaling. Since these genes are essential for development of mammary system, the aim of this study was to determine association of their polymorphism with milk production breeding values in Brown Swiss cattle. Blood of ninety milking cow were randomly obtained. DNA was extracted from whole blood using modified salting out method, then the desired fragments were PCR amplified and digested by specific restriction endonuclease enzymes. Gene and genotype frequencies, heterozygosity indexes, the real and effective allele number were calculated by PopGene software; and the breeding values of production traits were estimated by DFREML. SAS software was used to analyze association between genotypes and breeding values. The frequency of 'A' and 'C' alleles of POU1F1 and STAT5A were 0.455 and 0.489, respectively. This population was in hardy-weinburg equilibrium for both loci. There was no significant association between genotypes and breeding values, although POU1F1*B tended to produce higher milk and POU1F1*A showed higher fat and protein percent.

  11. Production of intraspecific F1 hybrids between wild and cultivated accessions of cowpea (Vigna unguiculata (L.) walp.) using conventional methods.

    Science.gov (United States)

    Lelou, B; Van Damme, P

    2006-01-01

    Cowpea (Vigna unguiculata (L.) Walp.) is an important food legume in the tropics. It belongs to the Phaseoleae (L.) tribe (Fabaceae family), it is diploid and its chromosome number is 22. Its gene pool includes the cultivated cowpea and its wild relatives, which are connected with Vigna subgenus, Catiang section. Cowpea has a great potential in increasing food legume production. The cowpea varieties, however, are susceptible to a number of insect pests, especially the pod borer Maruca testulalis and a pod sucking-bug complex (e.g.: Clavigralla tomentosicollis, Anoplocnemis curvipes and Riptortus dentipes), which cause severe damage. The crossing programme presented here exploits the variability existing in the wild African germplasm of V. unguiculata and cultivated cowpea. To incorporate the insect pest resistance into the cultivated cowpea economically, reciprocal crosses between wild forms and cowpea varieties were performed, using the stigmatic pollination methods at anthesis. Some barriers were found in these intraspecific crosses. In the majority of reciprocal crosses, the growth of the pollen tubes was arrested in the stigmatic tissue. Only 16.01% of the ovules were fertilised. In these ovules, embryo development was normal at about 20-25 days after pollination. The failure of the intraspecific crosses in about 80.7% of the cases is thus the result of the lack of fertilisation and the unfertilised ovules. There seems to exist considerable incompatibility within the primary cowpea gene pool. The breeding programme carried out under controlled conditions has proved to be less successful in developed cowpea intraspecific F1 hybrids. Further studies should concentrate on germplasm from Africa with documented resistance to major insect pests. In addition, the application of techniques for bypassing barriers to hybridisation of parent genotypes should enable these embryos to grow to plants.

  12. Production of Hybrid F1 Between Avena magna and Avena nuda and It's Identification%四倍体大燕麦×六倍体裸燕麦的杂种F1的产生及鉴定

    Institute of Scientific and Technical Information of China (English)

    赵云云; 周小梅; 杨才

    2003-01-01

    本研究以四倍体大燕麦(Avena magna L.)做母本,六倍体裸燕麦(Avena nuda L.)做父本进行杂交,利用幼胚拯救技术获得了杂种F1,并对其后代形态特征进行了观察;对杂种F1同工酶图谱和DNA指纹图谱进行了分析.杂种F1形态特征偏亲本或介于双亲之间;同工酶研究表明多数F1具有双亲互补酶带;RAPD分析不同引物扩增产物F1呈共显性或偏父、偏母.这些结果表明F1为真杂种.

  13. Eficiência bioeconômica de cordeiros F1 Dorper x Santa Inês para produção de carne Bioeconomic efficiency of F1 Dorper x Santa Inês lambs for meet production

    Directory of Open Access Journals (Sweden)

    Nelson Nogueira Barros

    2005-08-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência bioeconômica de cordeiros F1 Dorper x Santa Inês para produção de carne. Analisou-se o desempenho de ovinos ½ sangue Dorper x ½ sangue Santa Inês, nas fases de cria e de acabamento. A fase de produção das crias foi realizada em caatinga nativa e a fase de acabamento em confinamento. As matrizes foram suplementadas nos últimos 50 dias de prenhez e nos primeiros 30 dias de lactação. As crias foram desmamadas aos 70 dias de idade, divididas em três lotes e confinadas, alimentadas com capim-elefante (Pennisetum purpureum ad libitum e concentrado na proporção de 1,5%, 2,5% e 3,5% do peso vivo, respectivamente. O sexo não exerceu influência sobre os pesos no nascimento, no desmame, nem sobre o ganho em peso até o desmame. Não foi observada influência do sexo sobre os pesos e os ganhos em peso aos 30 e 50 dias de confinamento. Nas fases de produção e acabamento em confinamento, os animais de nascimento simples foram superiores aos de nascimento duplo quanto a essas variáveis. Houve efeito linear significativo para peso e ganho em peso aos 30 e 50 dias de confinamento. Os três níveis de uso de concentrado foram economicamente viáveis. As margens brutas de peso vivo, por kg de cordeiro produzido, foram de R$ 0,26 kg-1, R$ 0,30 kg-1 e R$ 0,36 kg-1 para concentrados a 1,5%, 2,5% e 3,5% do peso vivo, respectivamente. Os melhores resultados econômicos foram obtidos quando o nível de concentrado foi de 3,5% do peso vivo.The objective of this work was to evaluate bioeconomic efficiency of F1 Dorper x Santa Inês lambs for meet production. Performance of F1 Dorper x Santa Inês lambs in raising and fattening phases was analyzed. The production phase of lambs was realized in native pastures ("caatinga" and the finishing phase was in confinement. The ewes were supplemented during the last 50 days of pregnancy and the first 30 days of lactation. Lambs were weaned at 70 days of age

  14. General characteristics and suppression tuning properties of the distortion-product otoacoustic emission 2f1-f2 in the barn owl.

    Science.gov (United States)

    Taschenberger, G; Manley, G A

    1998-09-01

    The distortion-product otoacoustic emission (DPOAE) 2f1-f2 was measured in the ear canal of the barn owl. DPOAE were elicited by primary tones in 11 frequency regions from 1 to 9 kHz. The highest DPOAE output levels and best thresholds were found for f1 frequencies of 4 to 7 kHz and additionally at the lowest f1 frequency investigated. In some cases, the DPOAE sound pressures were only 37 dB below the primary-tone levels (PTL). The optimal primary-tone frequency ratios ranged from 1.05 to 1.45 and varied strongly among the different frequency regions investigated. The largest optimal ratios were measured in the middle frequency range for f1. At lower and higher f1, the optimal ratios decreased. DPOAE levels could be suppressed in a frequency-selective way by adding a third tone. As in other non-mammals, the best suppressive frequencies were near f1, suggesting DPOAE generation near the frequency place of this primary tone. This is in contrast to what is known for mammalian species, where the DPOAE is thought to be generated near f2. To obtain 6 dB of suppression of the DPOAE level, suppressor-tone levels ranging from 13 dB below to 4 dB above the primary-tone level were necessary. The Q10dB-values of suppression tuning curves increased as a function of frequency up to a value of 15.8. This tendency resembled the increase in frequency selectivity of auditory nerve fibers in this species.

  15. 不同群体山女鳟及其杂交F1的生产性能比较%Production Performance of Different Populations of Rainbow Trout Oncorhynchus masou masou and Their Cross-breeding F1

    Institute of Scientific and Technical Information of China (English)

    张玉勇; 张永泉; 贾智英; 贾忠贺; 白庆利

    2013-01-01

    本文系统研究了我国引进的两个山女鳟(Oncorhynchus masou masou)群体及其杂交F1代的生产性能.结果表明,(1)两个杂交组合的早期生产性能,如授精率、发眼率和孵化率等不存在显著优势,自交组合BH 08♀×BH08♂的最终成活率为63.37%,远低于其他三组的成活率(P<0.01);(2)378d的生长对比试验表明,杂交组合与自交组合的体质量、绝对增重率、特定生长率和肥满度等存在一定差异,养殖时间对这些指标的影响达到极显著水平(P<0.01),但组间差异仅为显著水平(P<0.05)或没有差异(P>0.05);(3)378d的生长对比试验结束时,两个杂交组合均表现出一定的杂种优势.方差分析表明,不同生长阶段对杂交组合杂种优势率的影响极显著(P<0.01),不同组合间的杂种优势率也存在极显著差异(P<0.01).%In this paper, the production performance were studied in two populations of rainbow trout Oncorhynchus masou masou and their cross-breeding Fl. The results showed that firstly there were no significant differences in production performance including fertil ization, eyed embryonic rate, and hatching success between the cross-breeding F1 (BH 96 9 ♀×BH 08 6 and BH 08 9 ♂ BH 96 5 ) and the controls (BH 96 ♀× BH 96 6 and BH 08 9 × BH 08 5 (P>0.05), with survival rate of only 63.3% in BH 08 9 ♀× BH 08 ♂ , very significantly lower than in the others (P0.05 ).Thirdly after a 378-day experi ment the cross-breeding Fl appeared some growth heterosis. The analysis of variance showed that the heterosis difference was affected not only by culture-time, but also among different groups (P<0.01).

  16. The Effect of Yerba Mate ( Supplementation on the Productive Performance of Dorper Ewes and Their Progeny

    Directory of Open Access Journals (Sweden)

    Eleonora Po

    2012-07-01

    Full Text Available Yerba Mate (Ilex paraguariensis, a tea known for its high antioxidant content, was supplemented to 30 of 60 ewes for 13 wks to assess its effect on their productive performance. A 2.5% inclusion rate of Yerba Mate (YM in a pelleted concentrate diet decreased feed intake and live weight (LW during the first few weeks post partum (p<0.001. Overall, the YM group ate less (2,092±78 g/d pellet than the control (CTRL one (2,434±83 g/d; similarly, LW was lower in the YM group compared to the CTRL one, 64.9±1.6 kg and 67.3±1.4 kg, respectively. Lambs’ birth weight and growth rates were not affected. At birth, lambs’ LW were similar between the Yerba Mate and control groups (4.2±0.5 kg and 4.1±0.4 kg, respectively. At the end of the trial, Yerba Mate lambs weighed 15.7±0.4 kg while CTRL lambs weighed 16.1±0.4 kg. Average daily growth rate was similar between the two groups and ranged from 176±19 to 234 ±24 g/d. The inclusion of Yerba Mate in a pelleted diet increased milk fat, protein and total solids content while it decreased milk lactose content. Further work is required to investigate the mechanisms by which Yerba Mate supplementation affects feed intake and milk composition.

  17. An Lck-cre transgene accelerates autoantibody production and lupus development in (NZB × NZW)F1 mice.

    Science.gov (United States)

    Nelson, R K; Gould, K A

    2016-02-01

    Lupus is an autoimmune disease characterized by the development of antinuclear autoantibodies and immune complex-mediated tissue damage. T cells in lupus patients appear to undergo apoptosis at an increased rate, and this enhanced T cell apoptosis has been postulated to contribute to lupus pathogenesis by increasing autoantigen load. However, there is no direct evidence to support this hypothesis. In this study, we show that an Lck-cre transgene, which increases T cell apoptosis as a result of T cell-specific expression of cre recombinase, accelerates the development of autoantibodies and nephritis in lupus-prone (NZB × NZW)F1 mice. Although the enhanced T cell apoptosis in Lck-cre transgenic mice resulted in an overall decrease in the relative abundance of splenic CD4(+) and CD8(+) T cells, the proportion of activated CD4(+) T cells was increased and no significant change was observed in the relative abundance of suppressive T cells. We postulate that the Lck-cre transgene promoted lupus by enhancing T cell apoptosis, which, in conjunction with the impaired clearance of apoptotic cells in lupus-prone mice, increased the nuclear antigen load and accelerated the development of anti-nuclear autoantibodies. Furthermore, our results also underscore the importance of including cre-only controls in studies using the cre-lox system.

  18. Efeito da restrição alimentar no desempenho produtivo e econômico de cabritos F1 Boer x Saanen Effect of feed restriction on economical and productive performances of F1-Boer x Saanen goats

    Directory of Open Access Journals (Sweden)

    José Morais Pereira Filho

    2005-02-01

    Full Text Available Objetivou-se, com este trabalho, avaliar o efeito dos níveis de restrição alimentar (0, 30 e 60% sobre o desempenho produtivo e econômico de cabritos F1 Boer x Saanen. Foram utilizados 21 cabritos inteiro, com média de 15,44 ± 0,37 kg de peso vivo (PV. Os animais do nível de restrição 0%, quando atingiram 25 kg de PV, foram submetidos a jejum (sólido de 24 horas e hídrico de 16 horas e abatidos com seus pares. A análise econômica foi referente, exclusivamente, aos custos com alimentação (U$1,00:R$3,70. A eficiência alimentar dos animais submetidos a 0 e 30% de restrição não diferiu. A restrição teve efeito linear decrescente para renda bruta média, quadrática para margem bruta média e rentabilidade média. Houve redução do custo da alimentação por kg de corpo vazio, de carcaça quente e de carcaça fria. A restrição em níveis moderados manteve a eficiência alimentar e reduziu as perdas com alimentação. A criação de cabrito leiteiro para abate entre 20 e 25 kg de PV, com restrição a partir dos 15 kg, aumentou a rentabilidade sobre o investimento em alimentação até o nível de 15,59% e diminuiu os custos com alimentação por kg de carcaça fria até o nível de 16,13% de restrição, caracterizando-se como alternativa ao sacrifício desses animais e à agregação de valor ao sistema de produção. Por outro lado, a compra de cabritos a preço de mercado para terminação até 25 kg de PV caracterizou-se como atividade de risco.The goal of this study was to evaluate the effect of different feed restriction levels (0, 30 and 60% on economical and productive performances of F1-Boer x Saanen goats. Twenty-one non-castrated goats with 15.44 ± 0.37 kg of mean live weight (LW were used. When animals submitted to 0% of feed restriction reached 25 kg of LW they and their pair were slaughtered after 24 h of feed fasting and 16 h of water fasting. Economical analysis was performed considering exclusively feed costs

  19. The use of combining ability analysis to identify elite parents for Artemisia annua F1 hybrid production.

    Directory of Open Access Journals (Sweden)

    Theresa Townsend

    Full Text Available Artemisia annua is an important medicinal crop used for the production of the anti-malarial compound artemisinin. In order to assist in the production of affordable high quality artemisinin we have carried out an A. annua breeding programme aimed at improving artemisinin concentration and biomass. Here we report on a combining ability analysis of a diallel cross to identify robust parental lines for hybrid breeding. The parental lines were selected based on a range of phenotypic traits to encourage heterosis. The general combining ability (GCA values for the diallel parental lines correlated to the positive alleles of quantitative trait loci (QTL in the same parents indicating the presence of beneficial alleles that contribute to parental performance. Hybrids generated from crossing specific parental lines with good GCA were identified as having an increase in both artemisinin concentration and biomass when grown either in glasshouse or experimental field trials and compared to controls. This study demonstrates that combining ability as determined by a diallel cross can be used to identify elite parents for the production of improved A. annua hybrids. Furthermore, the selection of material for breeding using this approach was found to be consistent with our QTL-based molecular breeding approach.

  20. The Effect of Holstein X Meat Cattle Breeds Crosses(F1 on Meat Production Increasing in Small Scale Farms Conditions

    Directory of Open Access Journals (Sweden)

    L. Papa

    2010-01-01

    Full Text Available About 80 % of Albanian small scale farms farming 1-2 dairy cows. The small agriculture land surface on farm property (1-1,5 ha is the main limiting factor for cow’s number increasing. So the farmer interest is not to increasing the number of cows in their farm but to fattening of calf’s born both for nutritive need of the family and market. The main objective of the study was to show the effect of industrial crosses (F1 of Holstein Frison cows with meat breeds like as Piementese(P, Limousine(L, Kianina(C, Sharole(Sh and Markixhana(M on meat production increasing on small scale farms. To carried out this objective, two study methods were used: (i The survey - 284 small scale farms was observed in the Albanian coastal field and (ii Comparative essay of fattening calves (F1 in semi intensive small scale farm condition - 32 calves, F1 crosses of Holstein cows with above mentioned meat breeds were put in fattening in semi intensive small scale farm condition. 6 Holstein breed (H calves of 3-4 months age was used as control group. The feeding of animals was based on forages produced in farm: corn silage 30 % D.M, fresh alpha-alpha and its hay. 35 % of dry matter requirements were supplied by bought concentrate feedstuffs with 15 % crude protein on dry matter bases. The fattening period lasted 8 months . The average daily body weight increasing for each crosses and control group were respectively 889g (PxH, 998g (LxH, 850g (KxH, 1010g( ChxH, 953g (MxH and 702g (HxH. Multivariate analyses according to a linear model with constant factors ( genotype, sex, genotype x sex and covariance “live body weight in the beginning of experiment” showed that: (i The effect of crossbreds on average daily gain is evident under the small-scale farms conditions, also, (ii The average daily gain increases with 23 -38 % , depending on the type of crosses, (iii The crosses of dairy cattle with Limousine and Sharole result as most effective. The differences between F

  1. Gestational N-hexane inhalation alters the expression of genes related to ovarian hormone production and DNA methylation states in adult female F1 rat offspring.

    Science.gov (United States)

    Li, Hong; Zhang, Chenyun; Ni, Feng; Guo, Suhua; Wang, Wenxiang; Liu, Jing; Lu, Xiaoli; Huang, Huiling; Zhang, Wenchang

    2015-12-15

    Research has revealed that n-hexane can disrupt adult female endocrine functions; however, few reports have focused on endocrine changes in adult F1 females after maternal exposure during gestation. In this study, female Wistar rats inhaled 100, 500, 2500, or 12,500 ppm n-hexane for 4 h daily during their initial 20 gestational days. The F1 female offspring exhibited abnormal oestrus cycles. Compared with the controls, the in vitro-cultured ovarian granulosa cells of the 12,500 ppm group showed significantly reduced in vitro progesterone and oestradiol secretion. Elevated progesterone secretion was observed in the 500 ppm group, and decreased and significantly upregulated mRNA expression of the Star, Cyp11a1, Cyp17a1, and Hsd3b genes was observed in the 12,500 ppm and 500 ppm groups, respectively. The protein expression levels were consistent with the mRNA expression levels. Methylation screening of the promoter regions of these genes was performed using MeDIP-chip and confirmed by methylation-sensitive high-resolution melting (MS-HRM), and the observed methylation state changes of the promoter regions were correlated with the gene expression levels. The results suggest that the hormone levels in the female offspring after gestational n-hexane inhalation correspond to the expression levels and DNA methylation states of the hormone production genes.

  2. Fruiting Body Formation of Cordyceps militaris from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains

    Science.gov (United States)

    Shrestha, Bhushan; Han, Sang-Kuk; Sung, Jae-Mo

    2012-01-01

    Interest in commercial cultivation and product development of Cordyceps species has shown a recent increase. Due to its biochemical and pharmacological effects, Cordyceps militaris, commonly known as orange caterpillar fungus, is being investigated with great interest. Cultivation of C. militaris has been practiced on a large scale in order to fulfill a demand for scientific investigation and product development. Isolates of C. militaris can be easily established from both spores and tissue. For isolation of spores, ascospores released from mature stromata are trapped in sterile medium. Multi-ascospore isolates, as well as combinations of single ascospore strains, are used for production of fruiting bodies. Progeny ascospore strains can be isolated from artificial fruiting bodies, thus, the cycle of fruiting body production can be continued for a long period of time. In this study, we examined fruiting body production from multi-ascospore isolates and their progeny strains for three generations. F1 progeny strains generally produced a larger number of fruiting bodies, compared with their mother multi-ascospore isolates; however, F2 and F3 progeny strains produced fewer fruiting bodies. Optimum preservation conditions could help to increase the vitality of the progeny strains. In order to retain the fruiting ability of the strains, further testing of various methods of preservation and different methods for isolation should be performed. PMID:22870051

  3. Evaluation of the immunomodulatory effects of the disinfection by-product, sodium chlorite, in female B6C3F1 mice: a drinking water study.

    Science.gov (United States)

    Karrow, N A; Guo, T L; McCay, J A; Johnson, G W; Brown, R D; Musgrove, D L; Germolec, D R; Luebke, R W; White, K L

    2001-08-01

    Sodium chlorite is an inorganic by-product of chlorine dioxide formed during the chlorination of drinking water. Relatively little is known about the adverse health effects of exposure to sodium chlorite in drinking water. In this study, we evaluated sodium chlorite's immunomodulatory properties using female B6C3F1 mice and a panel of immune assays that were designed to evaluate potential changes in innate and acquired cellular and humoral immune responses. Female B6C3F1 mice were exposed to sodium chlorite in their drinking water (0, 0.1, 1, 5, 15, and 30 mg/L) for 28 days, and then evaluated for immunomodulation. Overall, minimal toxicological and immunological changes were observed after exposure to sodium chlorite. Increases in the percentages of blood reticulocytes, and the relative spleen weights were both observed at different sodium chlorite treatment levels; however, these increases were not dose-dependent. An increasing trend in the number of spleen antibody-forming cells was observed over the range of sodium chlorite concentrations. This increase was not, however, significant at any individual treatment level, and was not reflected by changes in serum IgM levels. A significant increase (26%) in the total number of splenic CD8+ cells was observed in mice treated with 30 mg/L of sodium chlorite, but not at the other concentrations. Splenic mixed leukocyte response and peritoneal macrophage activity were unaffected by sodium chlorite. Lastly, exposure to sodium chlorite did not affect natural killer cell activity, although a decrease in augmented natural killer cell activity (42%) was observed at the lowest sodium chlorite treatment level. These results suggest that sodium chlorite, within the range 0.1-30 mg/L, produces minimal immunotoxicity in mice.

  4. The leaf extract of Mallotus japonicus and its major active constituent, rutin,suppressed on melanin production in murine B16F1 melanoma简

    Institute of Scientific and Technical Information of China (English)

    Junsei; Taira; Eito; Tsuchida; Masatsugu; Uehara; Natsuko; Ohhama; Wakana; Ohmine; Takayuki; Ogi

    2015-01-01

    Objective: To find anti-melanogenesis materials used in whitening cosmetics.Methods: The ethanolic leaf extract of Mallotus japonicus(M. japonicus) having an anti-melanogenesis activity was separated by a sephadex LH-20 chromatography. Each fraction was measured for its tyrosinase inhibitory activity together with its polyphenol content using the Folin–Ciocalteu method. The anti-melanogenesis activity of the active fractions was determined by the melanin content in the murine B16F1 melanoma. The active fractions were put together due to similar constituents, and then separated by high performance liquid chromatography using a C-18 ODS column. The major antimelanogenesis compound was identified using1 H and13C-NMR and liquid chromatography-mass spectrometry.Results: The ethanolic leaf extract of M. japonicus showed an anti-tyrosinase activity with a high polyphenol content, resulting in suppression of melanin production in the B16F1 melanoma. The extract was separated and the active compound was identical as rutin based on the1 H,13C-NMR and liquid chromatography-mass spectrometry analysis data. In addition, the rutin treatment with cells reduced the melanin content in a concentration dependent manner without any cell toxicity. The leaf extract of M. japonicus containing rutin would be useful in whitening cosmetics for protection from UV-light exposure to be limiting the accumulation of melanin in skin.Conclusions: The leaf extract of M. japonicus and/or rutin isolated from the extract as a key whitening agent would be useful as a whitening cosmetic material for protecting against disorder skin due to melanin accumulation.

  5. The leaf extract ofMallotus japonicus and its major active constituent, rutin, suppressed on melanin production in murine B16F1 melanoma

    Institute of Scientific and Technical Information of China (English)

    Junsei Taira; Eito Tsuchida; Masatsugu Uehara; Natsuko Ohhama; Wakana Ohmine; Takayuki Ogi

    2015-01-01

    Objective:To find anti-melanogenesis materials used in whitening cosmetics. Methods: The ethanolic leaf extract ofMallotus japonicus (M. japonicus) having an anti-melanogenesis activity was separated by a sephadexLH-20 chromatography. Each fraction was measured for its tyrosinase inhibitory activity together with its polyphenol content using the Folin–Ciocalteu method. The anti-melanogenesis activity of the active fractions was determined by the melanin content in the murine B16F1 melanoma. The active fractions were put together due to similar constituents, and then separated by high performance liquid chromatography using a C-18 ODS column. The major anti-melanogenesis compound was identified using 1Hand13C-NMR and liquid chromatography-mass spectrometry. Results: The ethanolic leaf extract ofM. japonicus showed an anti-tyrosinase activity with a high polyphenol content, resulting in suppression of melanin production in the B16F1 melanoma. The extract was separated and the active compound was identical as rutin based on the1H,13C-NMR and liquid chromatography-mass spectrometry analysis data. In addition, the rutin treatment with cells reduced the melanin content in a concentration dependent manner without any cell toxicity. The leaf extract ofM. japonicus containing rutin would be useful in whitening cosmetics for protection from UV-light exposure to be limiting the accumulation of melanin in skin. Conclusions: The leaf extract ofM. japonicus and/or rutin isolated from the extract as a key whitening agent would be useful as a whitening cosmetic material for protecting against disorder skin due to melanin accumulation.

  6. Andes Hantavirus-Infection of a 3D Human Lung Tissue Model Reveals a Late Peak in Progeny Virus Production Followed by Increased Levels of Proinflammatory Cytokines and VEGF-A.

    Science.gov (United States)

    Sundström, Karin B; Nguyen Hoang, Anh Thu; Gupta, Shawon; Ahlm, Clas; Svensson, Mattias; Klingström, Jonas

    2016-01-01

    Andes virus (ANDV) causes hantavirus pulmonary syndrome (HPS), a severe acute disease with a 40% case fatality rate. Humans are infected via inhalation, and the lungs are severely affected during HPS, but little is known regarding the effects of ANDV-infection of the lung. Using a 3-dimensional air-exposed organotypic human lung tissue model, we analyzed progeny virus production and cytokine-responses after ANDV-infection. After a 7-10 day period of low progeny virus production, a sudden peak in progeny virus levels was observed during approximately one week. This peak in ANDV-production coincided in time with activation of innate immune responses, as shown by induction of type I and III interferons and ISG56. After the peak in ANDV production a low, but stable, level of ANDV progeny was observed until 39 days after infection. Compared to uninfected models, ANDV caused long-term elevated levels of eotaxin-1, IL-6, IL-8, IP-10, and VEGF-A that peaked 20-25 days after infection, i.e., after the observed peak in progeny virus production. Notably, eotaxin-1 was only detected in supernatants from infected models. In conclusion, these findings suggest that ANDV replication in lung tissue elicits a late proinflammatory immune response with possible long-term effects on the local lung cytokine milieu. The change from an innate to a proinflammatory response might be important for the transition from initial asymptomatic infection to severe clinical disease, HPS.

  7. Morphological parameters and genetic diversity of progenies from seed production areas and unimproved stands of teak (Tectona grandis L.f.) in India

    Institute of Scientific and Technical Information of China (English)

    N. Lyngdoh; Geeta Joshi; G. Ravikanth; R. Vasudeva; R. Uma Shaanker

    2013-01-01

    We compared morphological and genetic attributes of prog-eny obtained from three seed production areas (SPAs) and three corre-sponding unimproved stands (UIS) of teak (Tectona grandis L.f) at three broad teak growing regions in the state of Karnataka, India. In general, seed morphological parameters such as seed weight, seed size and seed emptiness were significantly superior in SPAs compared to UISs. Seed germination percentage was also higher in seeds from SPAs. Seedling performance measured at two monthly intervals for six months was ob-served to be superior in SPAs at two of the three regions compared. Although the genetic diversity of progeny of SPAs was lower than UIS at all regions, the values were moderately high. Germination percent was positively correlated with fruit weight and kernel weight per seed, while diversity indices were negatively correlated with fruit weight, fruit di-ameter and kernel weight per seed. SPAs proved important as a source of moderately improved planting material with no severe threat to the ge-netic diversity of future plantations.

  8. Individual differences and the reliability of 2F1-F2 distortion-product otoacoustic emissions: effects of time-of-day, stimulus variables, and gender.

    Science.gov (United States)

    Cacace, A T; McClelland, W A; Weiner, J; McFarland, D J

    1996-12-01

    Distortion product otoacoustic emissions (DPOAEs) measured from the ear canal can be a sensitive tool to detect changes in cochlear function over time. However, if multiple-measurement procedures are to be useful clinically, testing needs to be reliable and sources of variability within individuals should be known. Herein, the influence of time-of-day (TOD), stimulus frequency, stimulus sound pressure level (SPL), and gender were evaluated on 2f1-f2 DPOAE amplitude in 16 adult volunteers with normal hearing. The effects of oral temperature and resting-pulse rate were also assessed. This study demonstrated a TOD main effect, with a period approximating one cycle-per-day. The magnitude of this effect averaged less than one dB and was not dependent on stimulus (frequency or SPL) or participant variables (gender, oral temperature, or resting-pulse rate), nor was it synchronized to a particular point-in-time. Stimulus level and gender effects on DPOAEs across frequency were also observed. Using generalizability theory (GT), DP iso-level/frequency profiles (DPILFPs) were found to be reliable measures within-subjects over a contiguous 24-hour time period. Significant and reliable between-subject differences were also documented. This study demonstrates the influence of stimulus and participant variables, quantifies the within-subject reliability over a 24-hour time period, and confirms that significant and reliable between-subject differences exist on DPOAEs across frequency, SPL, and gender.

  9. 翘嘴鳊及其亲本肌肉营养成分分析%Muscle nutrients of the backcross progeny of female diploid F1 hybrid (blunt snout bream × topmouth culter) × male blunt snout bream and its parents

    Institute of Scientific and Technical Information of China (English)

    何周玲; 刘少军; 肖军; 胡方舟; 文明; 叶利海; 张纯; 徐康; 陶敏

    2014-01-01

    为研究新型杂交鱼翘嘴鳊(二倍体鲂鲌F1♀×团头鲂♂)的肌肉营养价值,实验运用生化方法测定和分析了翘嘴鳊、翘嘴红鲌、团头鲂、二倍体鲂鲌F1(团头鲂早×翘嘴红鲌♂)的肌肉营养成分,包括这些样品的水分、脂肪、蛋白质和灰分含量,以及脂肪酸组成和氨基酸组成.结果表明,翘嘴鳊与其原始亲本相比具有较高的蛋白质和较低的碳水化合物含量;脂肪酸分析发现翘嘴鳊的不饱和脂肪酸,尤其是油酸、DHA等含量显著高于其原始父母本和其他有关鱼类(P<0.05);翘嘴鳊肌肉中具有较高呈味氨基酸比例,该比例高于其父母本的相应比例.研究表明,翘嘴鳊是一种营养价值高、口感好的优质鱼类.

  10. Revising the $f_1(1420)$ resonance

    CERN Document Server

    Debastiani, V R; Liang, Wei-Hong; Oset, E

    2016-01-01

    We have studied the production and decay of the $f_1(1285)$ into $\\pi a_0(980)$ and $K^* \\bar K$ as a function of the mass of the resonance and find a shoulder around 1400 MeV, tied to a triangle singularity, for the $\\pi a_0(980)$ mode, and a peak around 1420 MeV with about 60 MeV width for the $K^* \\bar K$ mode. Both these features agree with the experimental information on which the $f_1(1420)$ resonance is based. In addition, we find that if the $f_1(1420)$ is a genuine resonance, coupling mostly to $K^* \\bar K$ as seen experimentally, one finds unavoidably about a 20\\% fraction for $\\pi a_0(980)$ decay of this resonance, in drastic contradiction with all experiments. Altogether, we conclude that the $f_1(1420)$ is not a genuine resonance, but the manifestation of the $\\pi a_0(980)$ and $K^* \\bar K$ decay modes of the $f_1(1285)$ at higher energies than the nominal one.

  11. Two-dimensional electrophoresis analysis of protein production during growth of Pseudomonas putida F1 on toluene, phenol, and their mixture.

    Science.gov (United States)

    Reardon, Kenneth F; Kim, Kee-Hong

    2002-07-01

    The protein profiles of Pseudomonas putida F1 during growth on toluene, phenol, and their mixture were examined by two-dimensional polyacrylamide gel electrophoresis. Although this bacterium uses the same catabolic pathway for both substrates, P. putida F1 produced specific sets of proteins in response to toluene and phenol as single or mixed substrates. Proteins associated with growth on these substrates could be classified into three categories: ten Group T proteins were associated with the degradation of toluene, seventeen Group P proteins were associated with the degradation of phenol, and one Group M protein was observed to be associated only with toluene-phenol mixture degradation. During growth on the mixture, the protein profile of the cells shifted from Group T proteins to Group P proteins. This correlated well with the substrate consumption pattern, in which toluene was consumed first and growth on phenol did not begin until the medium was nearly depleted of toluene. Individual Group T and Group P protein intracellular concentrations had different transients as the cells grew on the mixture; seven protein levels increased, four decreased, and sixteen reached a maximum and then declined. The Group M protein reached a concentration maximum near the time when growth on phenol began. Variations in the maintenance of these proteins were also noted. These results demonstrate that cells growing on a mixture of substrates undergo significant physiological changes. Further investigation of these changes is expected to shed light on the unusual biodegradation kinetics previously observed with this mixed-substrate system.

  12. Adaptabilidade e estabilidade da produção de borracha e seleção em progênies de seringueira Adaptability and stability of rubber production and selection in rubber tree progenies

    Directory of Open Access Journals (Sweden)

    Cecília Khusala Verardi

    2009-10-01

    Full Text Available Os objetivos deste trabalho foram determinar a interação genótipo x ambiente e a estabilidade e adaptabilidade da produção de borracha em progênies de seringueira (Hevea brasiliensis (Willd. ex A. Juss. Müll. Arg., bem como estimar o ganho genético com a seleção dos materiais mais produtivos. Foram utilizadas três populações de progênies de meios-irmãos de seringueira, cultivadas em três municípios do Estado de São Paulo. Os experimentos foram instalados em delineamento de blocos ao acaso com 22 progênies, 6 repetições e 10 plantas por parcela, com espaçamento de 1,5x1,5 m. Aos três anos de idade, as progênies foram avaliadas quanto à produção de borracha seca utilizando-se o teste precoce de produção Hamaker Morris-Mann. As análises de estabilidade e adaptabilidade foram obtidas por modelos lineares mistos Reml/Blup. A seleção das cinco progênies mais produtivas proporcionou ganho genético de 8,16%. A pequena variação de desempenho das progênies mais produtivas, nos três locais de plantio, indica boa estabilidade fenotípica dos genótipos. As três progênies mais produtivas apresentam superioridade de produção de 14 a 19% sobre a média geral, nos três ambientes. As progênies selecionadas nos três locais podem ser indicadas para utilização na sequência de programas de melhoramento genético e podem levar à maximização na produção de látex.The objectives of this work were to determine the genotype x environment interaction and the stability and adaptability of rubber production in progenies of Hevea brasiliensis (Willd. ex A. Juss. Müll. Arg., as well as to estimate the genetic gain with selection of the most productive materials. Three half-sib progeny populations of rubber were cultivated in three municipalities in the state of São Paulo. The experiments were installed in a randomized complete block design with 22 progenies, 6 replicates and 10 plants per plot, with spacing of 1.5x1.5 m

  13. FOLIAR NUTRIENT CONTENTS AND FRUIT YIELD IN CUSTARD APPLE PROGENIES

    Directory of Open Access Journals (Sweden)

    Paulo Sérgio Lima e Silva

    2009-01-01

    Full Text Available Foliar nutrient contents are evaluated in several fruit trees with many objectives. Leaf analysis constitutes a way of evaluating the nutritional requirements of crops. Due to the positive impact that fertilizers have on crop yields, researchers frequently try to evaluate the correlations between yield and foliar nutrient contents. This work's objective was to present fruit yields from the 4th to the 6th cropping seasons, evaluate foliar nutrient contents (on the 5th cropping season, and estimate the correlations between these two groups of traits for 20 half-sibling custard apple tree progenies. The progenies were evaluated in a random block design with five replicates and four plants per plot. One hundred leaves were collected from the middle third of the canopy (in height of each of four plants in each plot. The leaves were collected haphazardly, i.e., in a random manner, but without using a drawing mechanism. In the analysis of variance, the nutrient concentrations in the leaves from plants of each plot were represented by the average of four plants in the plot. Fruit yield in the various progenies did not depend on cropping season; progeny A4 was the most productive. No Spearman correlation was found between leaf nutrient concentrations and fruit yield. Increased nutrient concentrations in the leaves were progeny-dependent, i.e., with regard to Na (progenies FE5 and JG1, Ca (progeny A4, Mg (progeny SM7, N (progeny A3, P (progeny M, and K contents (progeny JG3. Spearman's correlation was negative between Na-Mg, Na-Ca, and Mg-P contents, and positive between Mg-Ca and N-K contents.

  14. Sterile Insect Technique and F1 Sterility in the European Grapevine Moth, Lobesia botrana

    Science.gov (United States)

    Saour, George

    2014-01-01

    Newly emerged adults of the European grapevine moth, Lobesia botrana (Denis and Schiffermuller) (Lepidoptera: Tortricidae), were irradiated with various doses of gamma radiation and crossed to unirradiated counterparts of the opposite sex. Fecundity was decreased when unirradiated females were mated with either 300- or 350-Gy-irradiated males. Adult males that were irradiated with 400 Gy and mated with unirradiated females retained a residual fertility of 2.7%. The radiation dose at which irradiated females were found to be 100% sterile when mated with unirradiated males was 150 Gy. The inherited effects in the F1 progeny of irradiated male parents were examined at 100, 150, and 200 Gy. Fecundity and fertility of the F1 progeny of males irradiated with 150 Gy and inbred or crossed with irradiated and unirradiated moths were also recorded. A significant reduction in fertility was observed when F1 males mated with either F1 or unirradiated females. According to sterility index, F1 females who mated with F1 males had greater sterility than when F1 females were crossed to 150-Gy-irradiated males. Based upon the results of this study, 150 Gy of gamma radiation would be the optimal dose to use in a sterile insect technique and F1 sterility program against L. botrana. PMID:25373155

  15. Influence of strain of sire on channel x blue F1 hybrid catfish embryo production and fingerling performance under commercial farm conditions.

    Science.gov (United States)

    Channel x blue hybrid catfish is presently the desired aquaculture species in US farm-raised catfish industry. Even though production and performance has improved over the last two decades, several production and performance parameters needs to be improved to maximize the genetic potential of this ...

  16. Regulation of immune responses by I-J gene products. II. Presence of Both I-Jb and I-Jk suppressor factors in (nonsuppressor x nonsuppressor) F1 mice.

    Science.gov (United States)

    Lei, H Y; Dorf, M E; Waltenbaugh, C

    1982-04-01

    Antigen-specific suppression to poly(Glu50-Tyr50) (GT) is under the control of two complementary immune suppressor (Is) genes located in the major histocompatibility (H-2) complex of the mouse. Suppressor strains of mice produce both suppressor T (Ts) cells and Ts-derived suppressor factors (TsF) that bear antigenic determinants of the I-J subregion of the H-2 complex. Nonsuppressor strains of mice, on the other hand, are not suppressed by GT preimmunization. These nonsuppressor mice, however, can be classified according to those that lack the ability to make GT-specific T cell-derived suppressor factor (GT-TsF) after GT injection (i.e., H-2a, I-Jk mice) and those that lack the ability to be suppressed by the appropriate GT-TsF (i.e., H-2b,g2, I-Jb mice). In the present study, we demonstrate that (H-2a x H-2b,g2)F1 hybrid mice produce distinct GT-specific suppressor factors of both parental I-J haplotypes. Moreover, only the I-Jb-bearing GT-TsF derived from these F1 hybrid mice is able to induce second-order suppressor cells (Ts2). This is consistent with the observation that injection of GT-TsF1 derived from C57BL/6 (I-Jb) mice into A/J (I-Jk) mice leads to the production of an antigen-specific I-Jk GT-TsF2. Our results suggest that Is gene complementation occurs through a different cellular mechanism that was previously observed for Ir gene complementation. Further, we show that complementing (non-suppressor X nonsuppressor)F1 hybrid mice produce an I-Jb (and not an I-Jk) GT-TsF1 and an I-Jk (not an I-Jb) GT-TsF2, thus suggesting a heterogeneity of Ia loci within the I-J subregion. Data presented in the present study suggest that there may be even more heterogeneity within the I-J subregion than has has been heretofore reported with regard to I-J expression on Ts.

  17. A Radon Progeny Deposition Model

    CERN Document Server

    Guiseppe, V E; Hime, A; Rielage, K; Westerdale, S

    2011-01-01

    The next generation low-background detectors operating underground aim for unprecedented low levels of radioactive backgrounds. Although the radioactive decays of airborne radon (particularly Rn-222) and its subsequent progeny present in an experiment are potential backgrounds, also problematic is the deposition of radon progeny on detector materials. Exposure to radon at any stage of assembly of an experiment can result in surface contamination by progeny supported by the long half life (22 y) of Pb-210 on sensitive locations of a detector. An understanding of the potential surface contamination from deposition will enable requirements of radon-reduced air and clean room environments for the assembly of low background experiments. It is known that there are a number of environmental factors that govern the deposition of progeny onto surfaces. However, existing models have not explored the impact of some environmental factors important for low background experiments. A test stand has been constructed to depos...

  18. In vitro embryo rescue culture of F1 progenies from crosses between different ploidy grapes.

    Science.gov (United States)

    Ji, W; Li, G R; Luo, Y X; Ma, X H; Wang, M; Ren, R

    2015-12-29

    Crossing different ploidy grapes is an effective way to obtain new seedless cultivars. Although embryo rescue has been extensively applied in breeding seedless and triploid grapes, only a few improved cultivars have been developed. Based on preliminary studies, we set five crosses between tetraploid and diploid grape varieties to obtain new hybrid triploid germplasms. Additionally, we compared two different methods of performing in vitro embryo rescue and sowing in the development of hybrid triploid grape plants. The results showed that the germination rate of hybrid seeds was much lower (0-22.8%) than that of the self-pollinated seeds (50.9-61.2%) obtained though the same method of in vitro culture. Meanwhile, the seed germination rates of all crosses obtain through in vitro culture (0-61.2%) were higher than those obtained through sowing (0-42.0%). Identification of ploidy level confirmed that three lines obtained from the crosses of 'Ruby Seedless (2x) x Black Olympia (4x)' and 'Big black (2x) x Kyoho (4x)' were triploid, and one line from the cross of 'Big black (2x) x Kyoho (4x)' was haploid, and the others were diploid, tetraploid, or aneuploidy plants. Moreover, 4 haploid and 42 triploid surviving grape seedlings were planted in a vineyard after propagation. Therefore, an efficient system of breeding triploid seedless grapes using embryo rescue was established and 42 hybrid triploid germplasms were obtained for use in future studies.

  19. Molecular Marker Assisted Selection for Yield-Enhancing Genes in the Progeny of Minghui63 × O.rufipogon

    Institute of Scientific and Technical Information of China (English)

    WANG Yue-guang; WANG Bin; DENG Qi-yun; LIANG Feng-shan; XING Quan-hua; LI Ji-ming; XONG Yue-dong; SUN Shi-mong; GUO Bao-tai; YUAN Long-ping

    2004-01-01

    Two yield-enhancing genes (yldl.1 and yld2.1) are located on chromosomes 1 and 2 respectively in a weedy relative of cultivated rice, Oryza rufipogon. SSR markers RM9 and RM166 are closely linked with the two loci respectively. Minghui63 (MH63) has been a widely used restoration line in hybrid rice production in China during the past two decades. The F1 of cross "MH63 × O.rufipogon" was backcrossed with MH63 generation by generation. RM9 and RM166 were used to select the plants from the progeny of the backcross populations. The results were as follows: (1) In BC2F1 population, the percentage of the individuals which have RM9 and RM166 amplified bands simultaneously was 12.2%, while in the BC3F1 population, that was 16.3%. (2) Among 400individuals of BC3F1, four yield-promising plants were obtained, with yield being 30% more than that of MH63. (3) The products amplified by primer RM166 in O. rufipogon and MH63 were sequenced. It was found that the DNA fragment sequence amplified by RM166 from MH63 was 101 bp shorter than that from O. rufipogon. The 101 bp sequence is a part of an intron of the PCNA (proliferating cell nuclear antigen) gene.

  20. Effects of pollen selection on progeny vigor in a Cucurbita pepo x C. texana hybrid.

    Science.gov (United States)

    Quesada, M; Winsor, J A; Stephenson, A G

    1996-05-01

    We examined the effects of pollen selection for rapid pollen-tube growth on progeny vigor. First, we crossed a wild gourd (Cucurbita texana) to a cultivated zucchini (Cucurbita pepo cv 'Black Beauty') to produce an F1 and then an F2 generation. Half of the F1 seeds were produced by depositing small loads of C. texana pollen onto the stigmas of C. pepo. These small pollen loads were insufficient to produce a full complement of seeds and, consequently, both the fast- and the slow-growing pollen tubes were permitted to achieve fertilization. An F2 generation was then produced by depositing small loads of F1 pollen onto stigmas of F1 plants. The F2 seeds resulting from two generations of small pollen loads are termed the non-selected line because there was little or no selection for pollen-tube growth rate on these plants. The other half of the F1 and F2 seeds were produced by depositing large pollen loads (>10 000 pollen grains) onto stigmas and then allowing only the first 1% or so of the pollen tubes that entered the ovary to fertilize the ovules. We did this by excising the styles at the ovary at 12-15 h after pollination. The resulting F2 seeds are termed 'the selected line' because they were produced by two generations of selection for only the fastest growing pollen tubes. Small pollen loads from the F2plants, both the selected and the non-selected lines, were then deposited onto stigmas of different C. pepo flowers, and the vigor of the resulting seeds was compared under greenhouse and field conditions. The results showed that the seeds fertilized by pollen from the selected line had greater vegetative vigor as seedlings and greater flower and fruit production as mature plants than the seeds fertilized by pollen from the non-selected line. This study demonstrates that selection for fast pollen-tube growth (selection on the microgametophyte) leads to a correlated increase in sporophyte (progeny) vigor.

  1. Relationship of proton motive force and the F(0)F (1)-ATPase with bio-hydrogen production activity of Rhodobacter sphaeroides: effects of diphenylene iodonium, hydrogenase inhibitor, and its solvent dimethylsulphoxide.

    Science.gov (United States)

    Hakobyan, Lilit; Gabrielyan, Lilit; Trchounian, Armen

    2012-08-01

    Rhodobacter sphaeroides MDC 6521 was able to produce bio-hydrogen (H(2)) in anaerobic conditions under illumination. In this study the effects of the hydrogenase inhibitor-diphenylene iodonium (Ph(2)I) and its solvent dimethylsulphoxide (DMSO) on growth characteristics and H(2) production by R. sphaeroides were investigated. The results point out the concentration dependent DMSO effect: in the presence of 10 mM DMSO H(2) yield was ~6 fold lower than that of the control. The bacterium was unable to produce H(2) in the presence of Ph(2)I. In order to examine the mediatory role of proton motive force (∆p) or the F(0)F(1)-ATPase in H(2) production by R. sphaeroides, the effects of Ph(2)I and DMSO on ∆p and its components (membrane potential (∆ψ) and transmembrane pH gradient), and ATPase activity were determined. In these conditions ∆ψ was of -98 mV and the reversed ∆pH was +30 mV, resulting in ∆p of -68 mV. Ph(2)I decreased ∆ψ in concentrations of 20 μM and higher; lower concentrations of Ph(2)I as DMSO had no valuable effect on ∆ψ. The R. sphaeroides membrane vesicles demonstrated significant ATPase activity sensitive to N,N'-dicyclohexylcarbodiimide. The 10-20 μM Ph(2)I did not affect the ATPase activity, whereas 40 μM Ph(2)I caused a marked inhibition (~2 fold) in ATPase activity. The obtained results provide novel evidence on the involvement of hydrogenase and the F(0)F(1)-ATPase in H(2) production by R. sphaeroides. Moreover, these data indicate the role of hydrogenase and the F(0)F(1)-ATPase in ∆p generation. In addition, DMSO might increase an interaction of nitrogenase with CO(2), decreasing nitrogenase activity and affecting H(2) production.

  2. Production performance, egg quality and biochemical parameters of three way crossbred chickens with reciprocal F1 crossbred chickens in sub-tropical environment

    Directory of Open Access Journals (Sweden)

    Tabinda Khawaja

    2013-03-01

    Full Text Available A total of 225 hens (18 weeks of age of each Rhode Island Red male x Fayoumi female (RIFI, Fayoumi male x Rhode Island Red female (FIRI and White Leghorn male x FIRI female (RLH were maintained on deep litter system for a period of 72 weeks of age. In floor pens, each crossbred chicken was randomly distributed between pens, with 21 to 24 birds of the same breed per pen (2.00 to 2.50 ft2/bird. The results revealed that the age of sexual maturity was lowest in RIFI followed by FIRI and RLH chickens. The highest egg production was obtained by three-way crossbred chickens (RLH as compared with two-way crossbred chickens (RIFI and FIRI. The highest egg weight was observed in RLH than those of FIRI and RIFI crossbred chickens. The internal egg quality parameters included yolk weight, albumen weight, yolk + albumen weight and albumen height were same in all crossbred chickens. There was non-significant (p>0.05 difference in blood glucose, triglyceride, cholesterol, calcium, protein, uric acid and ALP values among all crossbred chickens. It may be concluded that three-way crossbred chickens (RLH showed better egg traits than two-way crossbred chickens (RIFI and FIRI with lower mortality. The RIFI crossbred chickens achieved sexual maturity earlier than both FIRI and RLH crossbred chickens with lower egg traits.

  3. Productividad, consumo y eficiencia biológica en vacas Frisón Neozelandés y F1 (Jersey-Frisón Neozelandés paridas a fines de invierno en la X Región, Chile Productivity, intake and biological efficiency in New Zealand Friesian and F1 (Jersey-New Zealand Friesian cows calved during late winter in the Xth Region, Chile

    Directory of Open Access Journals (Sweden)

    H. González-Verdugo

    2005-01-01

    significativas (P = 0,0001 en favor de F1. Las mayores diferencias se observaron en producción de grasa (24,6% y PL corregida a 4% de materia grasa (20,5%. Los valores de CMS no difirieron significativamente (P = 0,1642. Sin embargo, al expresarlos en relación a 100 kg de PV el valor de F1 tendió a ser de mayor magnitud (P = 0,0615. La PL y sólidos lácteos por unidad de CMS fueron altamente significativas (P = 0,0284 a favor del F1. La mayor superioridad se obtuvo en materia grasa (17,4%, donde el F1 alcanzó un valor de 63,88 g/kg de CMS. El cuociente entre la producción de proteína láctea y proteína cruda ingerida arrojó valores de 32,70 y 29,33% (P = 0,0003. En F1 la valoración energética de la PL diaria representó un 37,13% de la energía metabolizable consumida, aumentando a 41,38% al corregir por el aporte de reservas corporales. Estos valores fueron menores (P = 0,0002 en las FN. Se concluyó que la mayor producción de sólidos lácteos, menor PV y mejor utilización de la proteína cruda y energía metabolizable le otorgan al F1 una mayor eficiencia biológica respecto del FN. Las aptitudes mencionadas le confieren a las mestizas ventajas comparativas en sistemas donde se premia la producción y contenido de sólidos lácteos.At Oromo Experimental Station, University of Chile, Xth Region, Chile (41º08’ S; 73º09’ W, 47 primiparous cows, 23 New Zealand Friesian (NZF and 24 Jersey-NZF (F1, were used to estimate productivity, intake and biological efficiency, under grazing conditions. The information was obtained in 3 experimental periods (21 days each one, at which the cows were at 66, 126 and 220 days of lactation, respectively. The animals were weighed daily in order to estimate mean live weight (LW and live weight changes. Milk production (MP was measured 9 times each period, and weekly, a sample of milk was used to measure fat and protein content. Pasture dry matter intake (PDMI was estimated relating metabolizable energy requirements (MER and

  4. 乌珠穆沁羊(♀)×萨福克羊(♂)杂交F1产肉性能分析%The Performance of Meat Production in Hybrid F1 Sheep of Ujumuqin sheep(♀)×Suffolk Sheep(♂)

    Institute of Scientific and Technical Information of China (English)

    刘锋

    2010-01-01

    @@ 近年来,兴安盟引进萨福克羊与乌珠穆沁羊杂交,杂交F1羊增重效果显著.据2002年与2003年测定,放牧条件下,108只杂交F1羊6月龄平均活重36.25kg,而31只乌珠穆沁羊6月龄平均活重27.60kg,平均增重8.65kg,差异极显著.

  5. Selection of carioca common bean progenies resistant to white mold

    Directory of Open Access Journals (Sweden)

    Renato Sérgio Batista Carvalho

    2013-01-01

    Full Text Available A backcross breeding program between commercial common bean cultivars (VC3 and M20 and sources of resistance(Ex-Rico 23 and G122 was conducted with a view toward selecting carioca (beige with brown stripes progenies resistant to whitemold. Forty-eight progenies (27 F2:6 BC1 and 21 F1:5 BC2 were evaluated for yield, growth habit, grain type and pathogen responseby two methodologies for assessment (“straw test” and “oxalic acid”. The methods were effective in discriminating the progenies,showing differing results, for they may assess different resistance mechanisms. Thus, they should be used together. Simultaneousselection for yield, growth habit, grain type and white mold resistance proved to be viable. The most appropriate strategy was to uselower intensities of selection and prioritize traits such as resistance and grain type, which are essential for commercial acceptance.Two progenies proved their superiority for the breeding program for they combine the traits of resistance and favorable grain type.

  6. F1的学前班

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ 从CART比赛进入F1 美国一直缺少比F1更低级别的方程式比赛,美国(一些其它美洲国家也是这样)车手想要进入F1通常会去欧洲参加比赛或者在美国尝试与F1接近的CART赛车.

  7. IDENTIFIKASI GEN AROMA PADA PROGENI-PROGENI BACKCROSS ANTARA VARIETAS CIHERANG DENGAN PANDAN WANGI

    Directory of Open Access Journals (Sweden)

    Djarot Sasongko Hami Seno

    2011-08-01

    Full Text Available Marker-assisted PCR has been considered as the most potential method for fragrant selection. RM223 is the only suitable marker to identify mutated badh2 gene of Pandan Wangi. This research applies RM223-assisted PCR in the introgression of fragrant gene (mutated badh2 of Pandan Wangi variety, to engineer non-transgenic fragrant variety with good agronomic traits as those of Ciherang. Gene introduction was carried out through site-directed crossing; Pandan Wangi was crossed and backcrossed to Ciherang until heterozygot BC5F1, followed by selfing to obtain homozygot BC5F2. RM223-assisted selection was conducted in each cross and backcross generation. RM223 was able to identify native, mutated and heterozygot badh2 of Ciherang, Pandan Wangi, and their cross/backcross progenies, respectively. Therefore, the introgression of mutated badh2 within progenies were observed, as well as the statues of badh2 gene (native/mutated and alleles (homozygot/heterozygot. Further backcross and selfing to obtain BC5F2 is in progress

  8. 基于体细胞组织培养的甘蓝型油菜核三系保持系绵7MB-1亲本繁殖与F1制种%Reproduction of the Three-line Genic Male Sterile Line Parent Mian 7MB-1 (Brasscia Napus L.) and Seed Production of F1 Based on Somatic Tissue Culture

    Institute of Scientific and Technical Information of China (English)

    蒙大庆; 陈军; 袁代斌; 张跃非; 郭子荣; 胥岚; 李芝凡; 蒲定福; 汤天泽; 贺启川

    2009-01-01

    [Objective] The aim was to study the reproduction of the three-line genic male sterile (GMS) line parent Mian 7MB-1 (B.Napus L.) and the seed production of F1 through somatic tissue culture. [Method] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7%-93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500-15 000 hm2. [Conclusion] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.

  9. QTL analysis of the genetic architecture determining resistance to fire blight in an apple progeny

    NARCIS (Netherlands)

    Calenge, F.; Drouet, D.; Weg, van de W.E.; Brisset, M.N.; Paulin, J.P.; Durel, C.E.

    2004-01-01

    Fire blight, caused by the bacterial pathogen Erwinia amylovora, is one of the most destructive diseases of apple (Malus x domestica). In order to analyse the genetic determinism of resistance to fire blight in apple, a quantitative trait analysis (QTL) approach was used. A F1 progeny of 164

  10. QTL analysis of the genetic architecture determining resistance to fire blight in an apple progeny

    NARCIS (Netherlands)

    Calenge, F.; Drouet, D.; Weg, van de W.E.; Brisset, M.N.; Paulin, J.P.; Durel, C.E.

    2004-01-01

    Fire blight, caused by the bacterial pathogen Erwinia amylovora, is one of the most destructive diseases of apple (Malus x domestica). In order to analyse the genetic determinism of resistance to fire blight in apple, a quantitative trait analysis (QTL) approach was used. A F1 progeny of 164 individ

  11. Biochemical evaluation of triploid progenies of diploid × tetraploid breeding populations of Camellia for genotypes rich in catechin and caffeine.

    Science.gov (United States)

    Das, Sourabh Kumar; Sabhapondit, Santanu; Ahmed, Giasuddin; Das, Sudripta

    2013-06-01

    To verify the quality of triploid varieties of Camellia tea species at the secondary metabolite level, we tested caffeine and catechin profiles of 97 F(1) segregating progenies in two breeding populations with a common tetraploid parent and diploid parents of two geographic and varietal origins. Catechin and caffeine levels of the triploid progenies were quantified and compared against their diploid parent. Some of the progenies showed better performance than their diploid parent. Most of the progenies of the diploid C. sinensis × tetraploid cross showed heterosis for caffeine and EGCG. Progenies of the C. assamica subsp. lasiocalyx × tetraploid cross showed heterosis for +C, EC, EGC, and TC. The genomic contributions of the diploid parent seem to be the main factor in the variation between the two populations. Our studies showed quantitative enhancement of some of the quality-related parameters in tea, providing a platform to refocus on this classical breeding approach for developing quality cultivars in tea.

  12. IZK OLIMP F1 - NEW BULGARIAN TOMATO VARIETY FOR PROCESSING

    Directory of Open Access Journals (Sweden)

    Daniela Ganeva

    2015-12-01

    Full Text Available The hybrid IZK Olimp F1 is developed by a team at the Maritsa Vegetable Crops Research Institute, Plovdiv as a result of hybridization between female line М-441 and male line R-469. The F1 hybrid was tested in the Executive Agency for Variety Testing, Field Inspection and Seed Control in 2009-2010. It was recognised as a new tomato F1 hybrid variety by the Expert commission in 2009 and has a certificate №10987/ 31.08.2012 issued by the Patent Office of Republic of Bulgaria. Hybrid IZK Olimp F1 is a determinate, high-yielding tomato variety for mid-early field production. The total yield and earliness of this F1 hybrid are close to those of the hybrid var. Vodolei F1 and exceeds the direct var. Bela and var. Zhaklin. The fruits are oval-elongated, with an average weight of 55-68 g, uniform red coloured, thick, firm, crack resistant, with small and low pedicle hole. Being with good chemical and technological properties this hybrid is suitable for processing.

  13. Suppression of F1 Male-Specific Lethality in Caenorhabditis Hybrids by cbr-him-8

    Directory of Open Access Journals (Sweden)

    Vaishnavi Ragavapuram

    2016-03-01

    Full Text Available Haldane’s Rule and Darwin’s Corollary to Haldane’s Rule are the observations that heterogametic F1 hybrids are frequently less fit than their homogametic siblings, and that asymmetric results are often obtained from reciprocal hybrid crosses. In Caenorhabditis, Haldane’s Rule and Darwin’s Corollary have been observed in several hybrid crosses, including crosses of Caenorhabditis briggsae and C. nigoni. Fertile F1 females are obtained from reciprocal crosses. However, F1 males obtained from C. nigoni mothers are sterile and F1 males obtained from C. briggsae die during embryogenesis. We have identified cbr-him-8 as a recessive maternal-effect suppressor of F1 hybrid male-specific lethality in this combination of species. This result implicates epigenetic meiotic silencing in the suppression of F1 male-specific lethality. It is also shown that F1 males bearing a C. briggsae X chromosome are fertile. When crossed to C. briggsae hermaphrodites or F1 females derived from C. briggsae hermaphrodites, viable F2 and backcross (B2 progeny were obtained. Sibling males that possessed a C. nigoni X chromosome were sterile. Therefore, the sterility of F1 males bearing a C. nigoni X chromosome must result from dysgenic interactions between the X chromosome of C. nigoni and the autosomes of C. briggsae. The fertility of F1 males bearing a C. briggsae X chromosome provides an opportunity to identify C. nigoni loci that prevent spermatogenesis, and hence hermaphroditic reproduction, in diplo-X hybrids.

  14. Main: E2F1OSPCNA [PLACE

    Lifescience Database Archive (English)

    Full Text Available E2F1OSPCNA S000396 21-May-2002 (last modified) uchi re2f-1 found in the promoter of rice PCN...ividing cells and tissue; E2F; PCNA; meristematic tissue; cell cycle; rice (Oryza sativa); tobacco (Nicotiana tabacum) GCGGGAAA ...

  15. The Resistance of Chinese Wild Vitis to Uncinula necator and its Inheritance in F1 Generation

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jian-xia; WANG Yue-jin; XU Yan

    2002-01-01

    By natural field identification, the resistance of Chinese wild Vitis to Uncinula necator and its inheritance in F1 generation were studied with 35 clones of 9 Chinese wild Vitis species, 171 F1 individuals of 4 inter-species cross between Chinese wild Vitis and Vitis vinifera cultivars, and 16 individuals of selfpollinated Chinese wild Vitis. Results showed that the phenotypes of resistance to Uncinula necator in Chinese wild Vitis and its F1 generation were rich and diverse. Based on the segregation of resisitance to Uncinula necator in the progenies resulted from both interspecific hybridization and self-pollination, of Chinese native wild Vitis species and clones were controlled by polygenes showing dominant independent heredity. Minor resistant genes were also exist in Chinese wild susceptible Vitis species and clones.

  16. Activation of Ftz-F1-Responsive Genes through Ftz/Ftz-F1 Dependent Enhancers

    Science.gov (United States)

    Field, Amanda; Xiang, Jie; Anderson, W. Ray; Graham, Patricia; Pick, Leslie

    2016-01-01

    The orphan nuclear receptor Ftz-F1 is expressed in all somatic nuclei in Drosophila embryos, but mutations result in a pair-rule phenotype. This was explained by the interaction of Ftz-F1 with the homeodomain protein Ftz that is expressed in stripes in the primordia of segments missing in either ftz-f1 or ftz mutants. Ftz-F1 and Ftz were shown to physically interact and coordinately activate the expression of ftz itself and engrailed by synergistic binding to composite Ftz-F1/Ftz binding sites. However, attempts to identify additional target genes on the basis of Ftz-F1/ Ftz binding alone has met with only limited success. To discern rules for Ftz-F1 target site selection in vivo and to identify additional target genes, a microarray analysis was performed comparing wildtype and ftz-f1 mutant embryos. Ftz-F1-responsive genes most highly regulated included engrailed and nine additional genes expressed in patterns dependent on both ftz and ftz-f1. Candidate enhancers for these genes were identified by combining BDTNP Ftz ChIP-chip data with a computational search for Ftz-F1 binding sites. Of eight enhancer reporter genes tested in transgenic embryos, six generated expression patterns similar to the corresponding endogenous gene and expression was lost in ftz mutants. These studies identified a new set of Ftz-F1 targets, all of which are co-regulated by Ftz. Comparative analysis of enhancers containing Ftz/Ftz-F1 binding sites that were or were not bona fide targets in vivo suggested that GAF negatively regulates enhancers that contain Ftz/Ftz-F1 binding sites but are not actually utilized. These targets include other regulatory factors as well as genes involved directly in morphogenesis, providing insight into how pair-rule genes establish the body pattern. PMID:27723822

  17. Plant Regeneration of Tagetes patula Hybrid Progeny F1%孔雀草杂交F1代的植株再生

    Institute of Scientific and Technical Information of China (English)

    刘娟旭; 王静; 余义勋

    2009-01-01

    以孔雀草(Tagetes patula)子叶、下胚轴和叶片为外植体,通过器官直接发生途径诱导形成不定芽,探讨植物生长调节剂组合、AgNO3浓度、蔗糖浓度和外植体类型等因素对植株再生的影响,建立了再生体系.结果表明:MS+6-BA 1.0 mg·L-1 +NAA 0.5 mg·L-1 +AgNO3 1.0 mg·L-1+蔗糖40 g·L-1培养基最适合不定芽的分化和增殖,子叶不定芽分化率达90%以上,平均每外植体分化不定芽数达5.3个.不定芽较适生根培养基为1/2MS+IAA 0.2 mg·L-1+NAA 0.1 mg·L-1,生根率达到90%.

  18. The role of viral infection in inducing variability in virus-free progeny in tomato.

    Science.gov (United States)

    Marii, Liliana; Chiriac, Gheorghe

    2009-05-01

    The effect of virus-host interactions on subsequent generations is poorly understood. The evaluation of the effects of viral infection on inheritance of quantitative traits in the progeny of infected plants and elucidation of a possible relationship between chiasma frequency in the infected plants and variability of traits in the progeny were investigated. The current study involved genotypes of four intraspecific hybrids of tomato (Solanum lycopersicum L.), their parental forms and two additional cultivars. Used as infection were the tobacco mosaic virus (TMV) and potato virus X (PVX). The consequences of the effect of viral infection were evaluated based on chromosome pairing in diakinesis and/or by examining quantitative and qualitative traits in the progeny of the infected tomato plants. Tomato plants infected with TMV + PVX were found to differ in chiasma frequency per pollen mother cell or per bivalent. Deviations have been observed for genotypes of both F(1) hybrids and cultivars. At the same time, differences in mean values of the traits under study have only been found for progeny populations (F(2)-F(4)) derived from virus-infected F(1) hybrids, but not in the case of progeny of the infected cultivars. The rate of recombinants combining traits of both parents increased significantly (2.22-8.24 times) in progeny populations of hybrids infected with TMV + PVX. The above suggests that the observed effects could be the result of modification of recombination frequencies that can be manifested in heterozygous hybrids and make small contributions to variability in cases of 'homozygous' tomato genotypes (i.e. cultivars).

  19. The Role of Viral Infection in Inducing Variability in Virus-Free Progeny in Tomato

    Institute of Scientific and Technical Information of China (English)

    Liliana Marii; Gheorghe Chiriac

    2009-01-01

    The effect of virus-host interactions on subsequent generations is poorly understood. The evaluation of the effects of viral infection on inheritance of quantitative traits in the progeny of infected plants and elucidation of a possible relationship between chiasma frequency in the infected plants and variability of traits in the progeny were investigated. The current study involved genotypes of four intraspecific hybrids of tomato (Solanum lycopersicum L.), their parental forms and two additional cultivars. Used as infection were the tobacco mosaic virus (TMV) and potato virus X (PVX). The consequences of the effect of viral infection were evaluated based on chromosome pairing in diakinesis and/or by examining quantitative and qualitative traits in the progeny of the infected tomato plants. Tomato plants infected with TMV + PVX were found to differ in chiasma frequency per pollen mother cell or per bivalent. Deviations have been observed for genotypes of both F1 hybrids and cultivars. At the same time, differences in mean values of the traits under study have only been found for progeny populations (F2-F4) derived from virus-infected F1 hybrids, but not in the case of progeny of the infected cultivars. The rate of recombinants combining traits of both parents increased significantly (2.22-8.24 times) in progeny populations of hybrids infected with TMV + PVX. The above suggests that the observed effects could be the result of modification of recombination frequencies that can be manifested in heterozygous hybrids and make small contributions to variability in cases of 'homozygous' tomato genotypes (I.e. Cultivars).

  20. Estimation of genetic parameters in rubber progenies

    Directory of Open Access Journals (Sweden)

    Cecília Khusala Verardi

    2012-09-01

    Full Text Available This study was designed to evaluate the genetic variability, the potential for rubber yield and secondary traits of rubber tree progenies at three locations in the state of Sao Paulo. The experiments were conducted in a randomized block design with 22 progenies and 6 replications. At the age of three years, the progenies were evaluated for rubber yield, girth growth and total number of latex vessel rings. The results showed the existence of genetic variability among progenies for each location separately as well as between locations, with differences in the progeny performance for the traits. The individual heritabilities calculated for rubber yield, girth growth and total number of latex vessel rings (0.30, 0.63 and 0.29, respectively, associated with high genetic gains with selection for the traits studied at each site, showed that the populations can be considered suitable for the rubber breeding program, provided that an appropriate selection procedure is used.

  1. The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in F1 Hybrids

    Directory of Open Access Journals (Sweden)

    Tomoko Takamiya

    2009-01-01

    Full Text Available We analyzed inheritance of DNA methylation in reciprocal F1 hybrids (subsp. japonica cv. Nipponbare × subsp. indica cv. Kasalath of rice (Oryza sativa L. using restriction landmark genome scanning (RLGS, and detected differing RLGS spots between the parents and reciprocal F1 hybrids. MspI/HpaII restriction sites in the DNA from these different spots were suspected to be heterozygously methylated in the Nipponbare parent. These spots segregated in F1 plants, but did not segregate in selfed progeny of Nipponbare, showing non-Mendelian inheritance of the methylation status. As a result of RT-PCR and sequencing, a specific allele of the gene nearest to the methylated sites was expressed in reciprocal F1 plants, showing evidence of biased allelic expression. These results show the applicability of RLGS for scanning of non-Mendelian inheritance of DNA methylation and biased allelic expression.

  2. Reference: E2F1OSPCNA [PLACE

    Lifescience Database Archive (English)

    Full Text Available E2F1OSPCNA Kosugi S, Ohashi Y E2F sites that can interact with E2F proteins cloned from rice are require...d for meristematic tissue-specific expression of rice and tobacco proliferating cell nuclear antigen promoters Plant J 29: 45-59 (2002) PubMed: 12060226; ...

  3. Strategies for improving quality inspection of silkworm F1 hybrids egg products in Guangxi%广西桑蚕一代杂交种成品卵质量检验改进措施探讨

    Institute of Scientific and Technical Information of China (English)

    罗梅兰; 黄旭华; 安春梅; 胡文娟; 甘丽红

    2012-01-01

    [目的]探索桑蚕一代杂交种成品卵质量检验新方法,提高成品卵质量检验的准确性、高效性及可操作性.[方法]分别采用直接计数蚁蚕法、称量计算蚁蚕法、直接计数良卵法,进行蚕种孵化率和良卵数调查,并比较3种方法的调查效率.[结果]采用称量法计算蚁蚕头数与直接计数蚁蚕头数的结果无显著性差异,采用直接计数良卵数与实际良卵孵化值误差达到极显著水平;在效率方面,直接计数良卵法耗时是直接计数蚁蚕法的56.75%,而称量计算蚁蚕法耗时仅是直接计数蚁蚕法的21.83%,节省约4/5的调查时间,工作效率极大提高.[结论]将称量计算蚁蚕法应用于良卵数和孵化率调查是可行的,且具有操作性好、省时、省工、效率高,准确性高等优点.%[Objective]The present study was conduced to develop a new, accurate and efficient method for inspecting quality of silkworm F1 hybrids egg products. [ Method ] Hatching rate and number of good eggs were determined by direct counting of newly-hatched larva, weighing newly-hatched larva and direct counting of good eggs. These three methods were compared with each other for their efficiency. [Result]There was no significant difference between weighing and direct counting on newly-hatched larva, while significant difference occurred between the number of good eggs using direct counting method and the actual number of newly-hatched larva. For efficiency of the method, the time-consuming for direct counting of good eggs was 56.75% of direct counting of newly-hatched larva, while that of weighing method was only 21.83% of direct counting of newly-hatched larva, indicating that approximately 4/5 investigation time was saved so to enhance the work efficiency enormously. [Conclusion]Weighing newly-hatched larva method was found time and labor-saving, highly effective and accurate, and easy to be operated, hence it is feasible to apply weighing method to

  4. Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.

    Directory of Open Access Journals (Sweden)

    Diogo Teruo Hashimoto

    Full Text Available Juvenile fish trade monitoring is an important task on Brazilian fish farms. However, the identification of juvenile fish through morphological analysis is not feasible, particularly between interspecific hybrids and pure species individuals, making the monitoring of these individuals difficult. Hybrids can be erroneously identified as pure species in breeding facilities, which might reduce production on farms and negatively affect native populations due to escapes or stocking practices. In the present study, we used a multi-approach analysis (molecular and cytogenetic markers to identify juveniles of three serrasalmid species (Colossoma macropomum, Piaractus mesopotamicus and Piaractus brachypomus and their hybrids in different stocks purchased from three seed producers in Brazil. The main findings of this study were the detection of intergenus backcrossing between the hybrid ♀ patinga (P. mesopotamicus×P. brachypomus×♂ C. macropomum and the occurrence of one hybrid triploid individual. This atypical specimen might result from automixis, a mechanism that produces unreduced gametes in some organisms. Moreover, molecular identification indicated that hybrid individuals are traded as pure species or other types of interspecific hybrids, particularly post-F1 individuals. These results show that serrasalmid fish genomes exhibit high genetic heterogeneity, and multi-approach methods and regulators could improve the surveillance of the production and trade of fish species and their hybrids, thereby facilitating the sustainable development of fish farming.

  5. Measurements of size distributions of radon progeny for improved quantification of the lung cancer risk emanating from exposure to radon decay products; Messungen der Groessenverteilungen von Radon-Folgeprodukten zur Verbesserung der Quantifizierung des durch Radonexposition verursachten Lungenkrebsrisikos

    Energy Technology Data Exchange (ETDEWEB)

    Haninger, T.

    1997-12-31

    A major issue in radiation protection is to protect the population from the harmful effects of exposure to radon and radon progeny. Quantification of the lung cancer risk emanating from exposure to radon decay products in residential and working environments poses problems, as epidemiologic studies yield information deviating from the results obtained by the indirect method of assessment based on dosimetric respiratory tract models. One important task of the publication here was to characterize the various exposure conditions and to quantify uncertainties that may result from application of the ``dose conversion convention``. A special aerosol spectrometer was therefore designed and built in order to measure the size distributions of the short-lived radon decay products in the range between 0.5 nm and 10 000 nm. The aerosol spectrometer consists of a three-step diffusion battery with wire nets, an 11-step BERNER impactor, and a detector system with twelve large-surface proportional detectors. From the measured size distributions, dose conversion coefficients, E/P{sup eq}, were calculated using the PC software RADEP; the RADEP program was developed by BIRCHALL and JAMES and is based on the respiratory tract model of the ICRP. The E/P{sup eq} coefficients indicate the effective dose E per unit exposure P{sup eq} to radon decay products. (orig./CB) [Deutsch] Eines der groessten Probleme des Strahlenschutzes ist der Schutz der Bevoelkerung vor einer Strahlenexposition durch Radon und seine Folgeprodukte. Die Quantifizierung des Lungenkrebsrisikos, das durch Radonexpositionen in Wohnungen und an Arbeitsplaetzen verursacht wird, ist ein grosses Problem, weil epidemiologische Studien ein anderes Ergebnis liefern, als die indirekte Methode der Abschaetzung mit dosimetrischen Atemtrakt-Modellen. Eine wichtige Aufgabe der vorliegenden Arbeit war es, unterschiedliche Expositionsbedingungen zu charakterisieren und die Unsicherheiten zu quantifizieren, die sich aus der

  6. Measurements of size distributions of radon progeny for improved quantification of the lung cancer risk emanating from exposure to radon decay products; Messungen der Groessenverteilungen von Radon-Folgeprodukten zur Verbesserung der Quantifizierung des durch Radonexposition verursachten Lungenkrebsrisikos

    Energy Technology Data Exchange (ETDEWEB)

    Haninger, T.

    1997-12-31

    A major issue in radiation protection is to protect the population from the harmful effects of exposure to radon and radon progeny. Quantification of the lung cancer risk emanating from exposure to radon decay products in residential and working environments poses problems, as epidemiologic studies yield information deviating from the results obtained by the indirect method of assessment based on dosimetric respiratory tract models. One important task of the publication here was to characterize the various exposure conditions and to quantify uncertainties that may result from application of the ``dose conversion convention``. A special aerosol spectrometer was therefore designed and built in order to measure the size distributions of the short-lived radon decay products in the range between 0.5 nm and 10 000 nm. The aerosol spectrometer consists of a three-step diffusion battery with wire nets, an 11-step BERNER impactor, and a detector system with twelve large-surface proportional detectors. From the measured size distributions, dose conversion coefficients, E/P{sup eq}, were calculated using the PC software RADEP; the RADEP program was developed by BIRCHALL and JAMES and is based on the respiratory tract model of the ICRP. The E/P{sup eq} coefficients indicate the effective dose E per unit exposure P{sup eq} to radon decay products. (orig./CB) [Deutsch] Eines der groessten Probleme des Strahlenschutzes ist der Schutz der Bevoelkerung vor einer Strahlenexposition durch Radon und seine Folgeprodukte. Die Quantifizierung des Lungenkrebsrisikos, das durch Radonexpositionen in Wohnungen und an Arbeitsplaetzen verursacht wird, ist ein grosses Problem, weil epidemiologische Studien ein anderes Ergebnis liefern, als die indirekte Methode der Abschaetzung mit dosimetrischen Atemtrakt-Modellen. Eine wichtige Aufgabe der vorliegenden Arbeit war es, unterschiedliche Expositionsbedingungen zu charakterisieren und die Unsicherheiten zu quantifizieren, die sich aus der

  7. Linkage analysis and map construction in genetic populations of clonal F1 and double cross.

    Science.gov (United States)

    Zhang, Luyan; Li, Huihui; Wang, Jiankang

    2015-01-15

    In this study, we considered four categories of molecular markers based on the number of distinguishable alleles at the marker locus and the number of distinguishable genotypes in clonal F1 progenies. For two marker loci, there are nine scenarios that allow the estimation of female, male, and/or combined recombination frequencies. In a double cross population derived from four inbred lines, five categories of markers are classified and another five scenarios are present for recombination frequency estimation. Theoretical frequencies of identifiable genotypes were given for each scenario, from which the maximum likelihood estimates of one or more of the three recombination frequencies could be estimated. If there was no analytic solution, then Newton-Raphson method was used to acquire a numerical solution. We then proposed to use an algorithm in Traveling Salesman Problem to determine the marker order. Finally, we proposed a procedure to build the two haploids of the female parent and the two haploids of the male parent in clonal F1. Once the four haploids were built, clonal F1 hybrids could be exactly regarded as a double cross population. Efficiency of the proposed methods was demonstrated in simulated clonal F1 populations and one actual maize double cross. Extensive comparisons with software JoinMap4.1, OneMap, and R/qtl show that the methodology proposed in this article can build more accurate linkage maps in less time.

  8. Behavior of radon progeny in low frequency electromagnetic fields

    CERN Document Server

    Oda, K; Yamamoto, T

    1999-01-01

    Whether the electro-magnetic (EM) fields are carcinogenic or not still remains to be discussed from scientific point of view. Recently a possibility was pointed out that increased deposition of radon progeny in the EM-fields should enhance exposure dose to internal body. We investigated the behavior of charged sup 2 sup 2 sup 2 Rn progeny and aerosols containing them by measuring the pattern and the magnitude of the deposition rate of decay products on both CR-39 track detectors and imaging plates under various conditions. We concluded that the attachment to wire cables should be increased mainly by electric component of low frequency EM-fields and possibly by electric field induced by strong changing magnetic ones.

  9. The Meat Production Performance of the F1 Generation Hybrid Sheep of German Mutton Merino Sheep and Local Sheep%德国肉用美利奴羊×本地绵羊杂种羊产肉性能的研究

    Institute of Scientific and Technical Information of China (English)

    早热古丽·热合曼; 阿娜古丽·艾山; 阿力木江·斯拉木; 美丽坎木·依明; 阿瓦古丽·依明

    2015-01-01

    Abatract:In this study,the meat production performance of the F1 generation hybrid sheep of German Mutton Merino sheep and local sheep was evaluated. The result showed that the hybrid sheep have the heterosis in terms of adaptability to the local confinedness condition, growth and development and meat production performance in 2013 and 2014. The average weight of F 1 generation of the hybrid sheep in 6 months old was 44.24 kg and was significantly higher than that of the control group (P<0.01), and lean meat percentage was higher and mutton quality was better in 2014. The carcass weight of F1 generation in 6 months old was 6.28 kg higher than that of control group, and the meat percentage and dressing percentage of the F1 generation was 5.11%and 6.92%higher than those of the control group respectively (P<0.05).%对德国肉用美利奴羊(父本)与本地绵羊二元杂交后代羔羊的生长发育、产肉性能以及多胎基因等进行了研究。结果表明,2013年和2014年德×本F1杂交羔羊在当地的舍饲适应性、生长发育和产肉方面均表现出杂种优势,尤其是2014年6月龄德×本F1平均体重达到44.24 kg,比对照组高7.02 kg,差异极显著(P<0.01),且其瘦肉率高、羊肉品质好;经过短期育肥的6月龄杂种羔羊胴体重比对照组提高了6.28 kg,净肉率、屠宰率分别比对照组提高了5.11%和6.92%,差异显著(P<0.05)。

  10. Regulation of the thermoalkaliphilic F1-ATPase from Caldalkalibacillus thermarum

    Science.gov (United States)

    Ferguson, Scott A.; Cook, Gregory M.; Montgomery, Martin G.; Leslie, Andrew G. W.

    2016-01-01

    The crystal structure has been determined of the F1-catalytic domain of the F-ATPase from Caldalkalibacillus thermarum, which hydrolyzes adenosine triphosphate (ATP) poorly. It is very similar to those of active mitochondrial and bacterial F1-ATPases. In the F-ATPase from Geobacillus stearothermophilus, conformational changes in the ε-subunit are influenced by intracellular ATP concentration and membrane potential. When ATP is plentiful, the ε-subunit assumes a “down” state, with an ATP molecule bound to its two C-terminal α-helices; when ATP is scarce, the α-helices are proposed to inhibit ATP hydrolysis by assuming an “up” state, where the α-helices, devoid of ATP, enter the α3β3-catalytic region. However, in the Escherichia coli enzyme, there is no evidence that such ATP binding to the ε-subunit is mechanistically important for modulating the enzyme’s hydrolytic activity. In the structure of the F1-ATPase from C. thermarum, ATP and a magnesium ion are bound to the α-helices in the down state. In a form with a mutated ε-subunit unable to bind ATP, the enzyme remains inactive and the ε-subunit is down. Therefore, neither the γ-subunit nor the regulatory ATP bound to the ε-subunit is involved in the inhibitory mechanism of this particular enzyme. The structure of the α3β3-catalytic domain is likewise closely similar to those of active F1-ATPases. However, although the βE-catalytic site is in the usual “open” conformation, it is occupied by the unique combination of an ADP molecule with no magnesium ion and a phosphate ion. These bound hydrolytic products are likely to be the basis of inhibition of ATP hydrolysis. PMID:27621435

  11. EVALUATION OF THE REACTION OF WATERMELON PARENT AND F1 PLANTS TO Meloidogyne enterolobii

    Directory of Open Access Journals (Sweden)

    LÉIA SANTOS DAMACENO

    2016-01-01

    Full Text Available The aim of this study was to evaluate the performance of progenies from Citrullus lanatus var. lanatus (cultivated watermelons when crossed with progenies from C. lanatus var. citroides (fodder watermelon with a historic of resistance to the nematode Meloidogyne enterolobii. The parents and their F1s were evaluated for resistance to this nematode. In the initial stages of eleven treatments, watermelon seedlings plantlets were transplanted to plastic bags of six kilograms once the first leaves developed. Ten inoculated plants with 5,200 eggs in the soil near the stem of the plant and four non-inoculated ones were used in each treatment, in a complete block design. Sixty-two days after sowing, the following characteristics were evaluated: the length of the aerial part of the plant (LAP, in m, fresh mass of the aerial part (FMAP, in g, root fresh mass (RFM, in g, egg number (EN and reproduction factor (RF. A comparison between the averages of inoculated and non-inoculated plants was performed using Scott-Knott test at 5% and the diallelic analysis was performed using the GENES program. The morphological characteristics did not allow for the identification of the parent plants or the F1s with respect to nematode resistance, but the variables EN and RF were useful for such identification. The analyses of the general and specific combining abilities indicate highly significant effects with respect to this resistance, showing additive gene effects as well as dominance and epistatic gene effects, allowing for identification of parents and F1s that can be used in watermelon breeding programs to improve resistance to the M. enterolobii.

  12. Analysis list: Pou3f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pou3f1 Pluripotent stem cell + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou3f1....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou3f1.5.tsv http://dbarchive.biosc...iencedbc.jp/kyushu-u/mm9/target/Pou3f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou3f1.Plu

  13. Phytochemical profile of morphologically selected yerba-mate progenies

    Directory of Open Access Journals (Sweden)

    Alice Teresa Valduga

    2016-02-01

    Full Text Available ABSTRACT Yerba-mate (Ilex paraguariensis St. Hil is a native South American species. Plant progenies are populations that differ in terms of their productivity, morphology and phytochemical profile. This study aimed to determine the concentration of primary and secondary metabolites, such as antioxidants, in leaves, of yerba-mate progenies selected based on morphological characteristics. We evaluated the centesimal composition of secondary metabolites in the leaves of five yerba-mate plants. Methylxanthines and phenolic compounds were determined by UPLC-PDA, and antioxidant activity by measuring DPPH scavenging. Significant differences were found in centesimal composition and the contents of caffeine, theobromine, rutin and chlorogenic acid, as well as antioxidant activities, in selected progenies. The IC50 values were correlated with the chlorogenic acid levels (r2 = 0.5242 and soluble content (r2 = 0.7686. The morphological characteristics observed in yerba-mate leaves can be used as a tool for plant selection, to obtain matrices with different phytochemical profiles as a genetic material source.

  14. Selection of arabica coffee progenies tolerant to heat stress

    Directory of Open Access Journals (Sweden)

    Alexsandro Lara Teixeira

    2015-07-01

    Full Text Available Due to high temperatures, practically all coffee farms in the state of Rondonia are of the C. canephora species. Thus, importing arabica coffee from other states becomes necessary for composition of blends, as well as for the specialty or gourmet coffee market. The purpose of this study was to select arabica coffee genotypes that exhibit satisfactory agronomic performance under high temperature conditions. The experiment was conducted in OuroPreto do Oeste, RO, Brazil, with mean annual temperature of 25.8°C and mean annual rainfall of 2300mm year-1. The experiment was composed of 114 arabica coffee genotypes, with 103 progenies and eleven control cultivars, provided by EPAMIG. A randomized block experimental design was used with three replications, spacing of 3.0x1.0 meters and five plants per plot. All the crop seasons showed significant difference for the green coffee yield trait. In joint analysis, significant differences were detected among progenies and control cultivars. In the average of the four harvests, green coffee yield was 32.38 bags ha-1. The cultivars 'CatuaíVermelho IAC 15', 'Obatã IAC 1669-20' and 'Catucaí Amarelo 2SLCAK' stood out, achieving yields greater than 40 bags ha-1. The gain obtained from selection was 14.33 bags ha-1, which is equivalent to an increase of 44.04% in production of green coffee. The progeny H514-7-10-6-2-3-9 stood out with an average yield of 51.20 bags ha-1. In regard to maturation cycle, 56% of the progenies were classified as early maturity and 44% as medium maturity. Late maturity genotypes were not observed

  15. Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding

    Directory of Open Access Journals (Sweden)

    Lorena V. Armando

    2017-09-01

    Full Text Available Panicum coloratum var. makarikariense, a perennial grass native to Africa, is adapted to a wide range of soil and climatic conditions with potential to be used as forage in tropical and semi-arid regions around the world. Our objective was to understand how the pollination mode affects viable seed production and further survival of the progeny. We evaluated self- and open-pollinated progenies from different accessions by measuring the seed production of the parents and their germination performance, germination rate and seedling survival. Parents and progeny were also fingerprinted with Simple Sequence Repeats (SSR. Progeny produced through open-pollination resulted in significantly more filled seeds and superior seedling survival than self-pollination. These results indicate that accessions studied here rely heavily on cross-pollination, whereas the contribution of self-pollinated offspring to the population is likely to be low. SSR profiles showed that, on average, 85% of the progeny (arising from cross-pollination possessed paternal specific markers and 100% of them were genetically different from the maternal genotype. All plants examined had 4x = 36 chromosomes. Overall, our findings indicate that var. makarikariense is able to generate highly polymorphic progeny through segregation and recombination. This study provides reference information for the formulation of appropriate strategies for pasture germplasm management, conservation and development of breeding programs. 

  16. Single Low-Dose Ionizing Radiation Induces Genotoxicity in Adult Zebrafish and its Non-Irradiated Progeny.

    Science.gov (United States)

    Lemos, J; Neuparth, T; Trigo, M; Costa, P; Vieira, D; Cunha, L; Ponte, F; Costa, P S; Metello, L F; Carvalho, A P

    2017-02-01

    This study investigated to what extent a single exposure to low doses of ionizing radiation can induce genotoxic damage in irradiated adult zebrafish (Danio rerio) and its non-irradiated F1 progeny. Four groups of adult zebrafish were irradiated with a single dose of X-rays at 0 (control), 100, 500 and 1000 mGy, respectively, and couples of each group were allowed to reproduce following irradiation. Blood of parental fish and whole-body offspring were analysed by the comet assay for detection of DNA damage. The level of DNA damage in irradiated parental fish increased in a radiation dose-dependent manner at day 1 post-irradiation, but returned to the control level thereafter. The level of DNA damage in the progeny was directly correlated with the parental irradiation dose. Results highlight the genotoxic risk of a single exposure to low-dose ionizing radiation in irradiated individuals and also in its non-irradiated progeny.

  17. Analysis list: Pou5f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pou5f1 Embryonic fibroblast,Pluripotent stem cell + mm9 http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/target/Pou5f1.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou5f1.5.tsv h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou5f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou5f1....Embryonic_fibroblast.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou5f1

  18. Charmless hadronic $B \\to (f_1(1285),f_1(1420)) P$ decays in the perturbative QCD approach

    CERN Document Server

    Liu, Xin; Li, Jing-Wu; Zou, Zhi-Tian

    2014-01-01

    We study twenty charmless hadronic $B \\to f_1 P$ decays, with $f_1$ representing axial-vector mesons $f_1(1285)$ and $f_1(1420)$ that resulting from a mixing of quark-flavor $f_{1q}$ and $f_{1s}$ states with the angle $\\phi_{f_1}$, in the perturbative QCD(pQCD) formalism. The estimations of branching ratios and CP asymmetries of the considered $B \\to f_1 P$ decays are presented in the pQCD approach with $\\phi_{f_1} \\sim 24^\\circ$ from recently measured $B_{d/s} \\to J/\\psi f_1(1285)$ decays. It is found that (a) the tree dominant $B^+ \\to f_1 \\pi^+$ and the penguin dominant $B^+ \\to f_1 K^+$ decays with large branching ratios[${\\cal O}(10^{-6})$] and large direct CP violations(around $14\\% \\sim 28\\%$ in magnitude) simultaneously are believed to be clearly measurable at the LHCb and Super-B factory experiments; (b) the nearly pure penguin-dominated $B_d \\to f_1 K_S^0$ and $B_s \\to f_1 (\\eta, \\eta')$ modes with safely negligible tree pollution also have large decay rates in the order of $10^{-6} \\sim 10^{-5}$, w...

  19. 26 CFR 1.415(f)-1 - Aggregating plans.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Aggregating plans. 1.415(f)-1 Section 1.415(f)-1...) INCOME TAXES Pension, Profit-Sharing, Stock Bonus Plans, Etc. § 1.415(f)-1 Aggregating plans. (a) In general. Except as provided in paragraph (g) of this section (regarding multiemployer plans), and taking...

  20. Photoproduction of the $f_1(1285)$ Meson

    CERN Document Server

    Dickson, R

    2016-01-01

    The $f_1(1285)$ meson with mass $1281.0 \\pm 0.8$ MeV/$c^2$ and width $18.4 \\pm 1.4$ MeV (FWHM) was measured for the first time in photoproduction from a proton target using CLAS at Jefferson Lab. Differential cross sections were obtained via the $\\eta\\pi^{+}\\pi^{-}$, $K^+\\bar{K}^0\\pi^-$, and $K^-K^0\\pi^+$ decay channels from threshold up to a center-of-mass energy of 2.8 GeV. The mass, width, and an amplitude analysis of the $\\eta\\pi^{+}\\pi^{-}$ final-state Dalitz distribution are consistent with the axial-vector $J^P=1^+$ $f_1(1285)$ identity, rather than the pseudoscalar $0^-$ $\\eta(1295)$. The production mechanism is more consistent with $s$-channel decay of a high-mass $N^*$ state, and not with $t$-channel meson exchange. Decays to $\\eta\\pi\\pi$ go dominantly via the intermediate $a_0^\\pm(980)\\pi^\\mp$ states, with the branching ratio $\\Gamma(a_0\\pi \\text{ (no} \\bar{K} K\\text{)}) / \\Gamma(\\eta\\pi\\pi \\text{(all)}) = 0.74\\pm0.09$. The branching ratios $\\Gamma(K \\bar{K} \\pi)/\\Gamma(\\eta\\pi\\pi) = 0.216\\pm0.033$...

  1. Main: O2F1BE2S1 [PLACE

    Lifescience Database Archive (English)

    Full Text Available quences of be2S1 promoter; F1 is hybrid C/G box; O2; opaque-2; be2S1; F1; seed; Brazil nut tree (Bertholletia excelsa); TCCACGTCGA ... ...O2F1BE2S1 S000162 17-May-1998 (last modified) kehi opaque-2 recognition site F1 in Bertholletia excelsa (Bra...zil nut tree) 2S storage protein gene (be2S1); O2 protein binds to F1, F2 and F3 se

  2. Analysis list: E4f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available E4f1 Embryonic fibroblast + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E4f1....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E4f1.5.tsv http://dbarchive.biosciencedb...c.jp/kyushu-u/mm9/target/E4f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/E4f1.Embryonic_fibr

  3. Analysis list: Gtf2f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Gtf2f1 Blood + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Gtf2f1.1.t...sv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Gtf2f1.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Gtf2f1....10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Gtf2f1.Blood.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Blood.gml ...

  4. Analysis list: Pou2f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pou2f1 Blood + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou2f1.1.t...sv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou2f1.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou2...f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou2f1.Blood.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Blood.gml ...

  5. Segregation of a major gene influencing ovulation in progeny of Lacaune meat sheep

    Science.gov (United States)

    Bodin, Loys; SanCristobal, Magali; Lecerf, Frédéric; Mulsant, Philippe; Bibé, Bernard; Lajous, Daniel; Belloc, Jean-Pierre; Eychenne, Francis; Amigues, Yves; Elsen, Jean-Michel

    2002-01-01

    Inheritance of the ovulation rate (OR) in the Lacaune meat breed was studied through records from a small nucleus of 36 hyper-prolific ewes screened on farms on the basis of their natural litter size, and from progeny data of three selected Lacaune sires. These sires were chosen at the AI centre according to their breeding values estimated for the mean and the variability of their daughters' litter size. Non-carrier Lacaune dairy ewes were inseminated to produce 121 F1 daughters and 27 F1 sons. Twelve sons (four from each sire) were used in turn to inseminate non-carrier Lacaune dairy ewes providing 260 BC progeny ewes. F1 and BC progeny were brought from private farms and gathered after weaning on an experimental farm where ovulation rates were recorded in the first and second breeding seasons. With an average of 6.5 records each, the mean OR of hyper-prolific ewes was very high (5.34), and 38.4% of records showed a rate of 6 or more. F1 data showed high repeatability of OR (r = 0.54) within ewe, with significant variability among ewes. High OR (≥ 4) were observed in each family. A segregation analysis provided a significant likelihood ratio and classified the three founders as heterozygous. BC ewes also displayed high repeatability of OR (r = 0.47) and the mean OR varied considerably between families (from 1.24 to 1.78). Seven of the 12 BC families presented high-ovulating ewes (at least one record ≥ 4) and segregation analysis yielded a highly significant likelihood ratio as compared to an empirical test distribution. The high variability of the mean ovulation rate shown by a small group of daughters of BC ewes inseminated by putative carrier F1 rams, and the very high ovulation rate observed for some of these ewe lambs, confirmed the segregation of a major gene with two co-dominant alleles borne by an autosome. The difference between homozygous non-carriers and heterozygous ewes was about one ovulation on the observed scale and 2.2 standard deviations on

  6. Segregation of a major gene influencing ovulation in progeny of Lacaune meat sheep

    Directory of Open Access Journals (Sweden)

    Amigues Yves

    2002-07-01

    Full Text Available Abstract Inheritance of the ovulation rate (OR in the Lacaune meat breed was studied through records from a small nucleus of 36 hyper-prolific ewes screened on farms on the basis of their natural litter size, and from progeny data of three selected Lacaune sires. These sires were chosen at the AI centre according to their breeding values estimated for the mean and the variability of their daughters' litter size. Non-carrier Lacaune dairy ewes were inseminated to produce 121 F1 daughters and 27 F1 sons. Twelve sons (four from each sire were used in turn to inseminate non-carrier Lacaune dairy ewes providing 260 BC progeny ewes. F1 and BC progeny were brought from private farms and gathered after weaning on an experimental farm where ovulation rates were recorded in the first and second breeding seasons. With an average of 6.5 records each, the mean OR of hyper-prolific ewes was very high (5.34, and 38.4% of records showed a rate of 6 or more. F1 data showed high repeatability of OR (r = 0.54 within ewe, with significant variability among ewes. High OR (≥ 4 were observed in each family. A segregation analysis provided a significant likelihood ratio and classified the three founders as heterozygous. BC ewes also displayed high repeatability of OR (r = 0.47 and the mean OR varied considerably between families (from 1.24 to 1.78. Seven of the 12 BC families presented high-ovulating ewes (at least one record ≥ 4 and segregation analysis yielded a highly significant likelihood ratio as compared to an empirical test distribution. The high variability of the mean ovulation rate shown by a small group of daughters of BC ewes inseminated by putative carrier F1 rams, and the very high ovulation rate observed for some of these ewe lambs, confirmed the segregation of a major gene with two co-dominant alleles borne by an autosome. The difference between homozygous non-carriers and heterozygous ewes was about one ovulation on the observed scale and 2

  7. Seleção de progênies de erva-mate (Ilex paraguariensis St. Hill. para a produtividade, estabilidade e adaptabilidade temporal de massa foliar Selection of Erva-mate Progenies for Leaf Weight Productivity, Temporal Stability and Adaptability

    Directory of Open Access Journals (Sweden)

    José Alfredo Sturion

    2011-03-01

    Full Text Available O presente trabalho teve por objetivos selecionar progênies de erva-mate (Ilex paraguariensis St. Hil.  em Ivaí - PR, simultaneamente para estabilidade, adaptabilidade e produtividade de massa foliar,  colhidas em três safras, com base na média harmônica a performance relativa dos valores genéticos (MHPRVG, adicionalmente, comparar os resultados  com aqueles obtidos pelos métodos de Annicchiarico e  de Lin & Binns. O material genético consistiu de 25  progênies de meios-irmãos plantadas no delineamento  de blocos ao acaso, com dez repetições  e parcelas lineares de seis plantas no espaçamento de  3m x 2m. O peso de folhas foi avaliado, por árvore,  aos dois, quatro e seis anos após o plantio. Houve  uma alteração no ordenamento das progênies para a produção de massa foliar ao longo das safras. Os  métodos de Annicchiarico, Lin & Binns e MHPRVG  selecionaram, praticamente, as mesmas progênies para os três atributos considerados. Contudo, o método  HPRVG apresenta a vantagem de fornecer  resultados na própria escala e medição do caráter, os quais podem ser interpretados diretamente como  valores genéticos.
    This paper aimed to select progenies of “erva-mate” (Ilex paraguariensis St. HiI. in Ivai - PR based on leaf weight productivity, stability and adaptability across three harvests. This can be achieved by the method MHPRVG (harmonic average of relative performance of genetic values which is comparable with the methods by Annichiaricco and Lin & Binns. The genetic material was constituted by 25 half sib progenies evaluated in a complete block design with ten replicates and six plants per plot, in a 3 x 2 meters spacing. Leaf weight per tree  was evaluated at ages two, four and six years  after planting. There were some changes in progenies  ranking across harvests. The methods MHPRVG, Annichiaricco and Lin & Binns led to selection of the  same progenies, considering the three

  8. Tomato progeny inherit resistance to the nematode Meloidogyne javanica linked to plant growth induced by the biocontrol fungus Trichoderma atroviride

    Science.gov (United States)

    Medeiros, Hugo Agripino de; Araújo Filho, Jerônimo Vieira de; Freitas, Leandro Grassi de; Castillo, Pablo; Rubio, María Belén; Hermosa, Rosa; Monte, Enrique

    2017-01-01

    Root-knot nematodes (RKN) are major crop pathogens worldwide. Trichoderma genus fungi are recognized biocontrol agents and a direct activity of Trichoderma atroviride (Ta) against the RKN Meloidogyne javanica (Mj), in terms of 42% reduction of number of galls (NG), 60% of number of egg masses and 90% of number of adult nematodes inside the roots, has been observed in tomato grown under greenhouse conditions. An in vivo split-root designed experiment served to demonstrate that Ta induces systemic resistance towards Mj, without the need for the organisms to be in direct contact, and significantly reduces NG (20%) and adult nematodes inside tomato roots (87%). The first generation (F1) of Ta-primed tomato plants inherited resistance to RKN; although, the induction of defenses occurred through different mechanisms, and in varying degrees, depending on the Ta-Mj interaction. Plant growth promotion induced by Ta was inherited without compromising the level of resistance to Mj, as the progeny of Ta-primed plants displayed increased size and resistance to Mj without fitness costs. Gene expression results from the defense inductions in the offspring of Ta-primed plants, suggested that an auxin-induced reactive oxygen species production promoted by Ta may act as a major defense strategy during plant growth. PMID:28071749

  9. Behavior toxicity to Caenorhabditis elegans transferred to the progeny after exposure to sulfamethoxazole at environmentally relevant concentrations

    Institute of Scientific and Technical Information of China (English)

    Zhenyang Yu; Lei Jiang; Daqiang Yin

    2011-01-01

    Sulfamethoxazole (SMX) is one of the most common detected antibiotics in the environment. In order to study whether SMX can affect behavior and growth and whether these effects could be transferred to the progeny, Caenorhabditis elegans was exposed at environmentally relevant concentrations for 24, 48, 72 and 96 hr, respectively. After exposure, the exposed parent generation (Po) was measured for behavior and growth indicators, which were presented as percentage of controls (POC). Then their corresponding unexposed progeny (F1) was separated and measured for the same indicators. The lowest POC for Po after 96 hr-exposure at 100 mg/L were 37.8%, 12.7%, 45.8% and 70.1% for body bending frequency (BBF), reversal movement (RM), Omega turns (OT) and body length (BL), respectively. And F1 suffered defects with the lowest POC as 55.8%, 24.1%, 48.5% and 60.7% for BBF, RM, OT and BL, respectively. Defects in both Po and F1 showed a time- and concentration-dependent fashion and behavior indicators showed better sensitivity than growth indicator. The observed effects on F1 demonstrated the transferable properties of SMX. Defects of SMX at environmental concentrations suggested that it is necessary to perform further systematical studies on its ecological risk in actual conditions.

  10. E2F1-mediated human POMC expression in ectopic Cushing's syndrome.

    Science.gov (United States)

    Araki, Takako; Liu, Ning-Ai; Tone, Yukiko; Cuevas-Ramos, Daniel; Heltsley, Roy; Tone, Masahide; Melmed, Shlomo

    2016-11-01

    Cushing's syndrome is caused by excessive adrenocorticotropic hormone (ACTH) secretion derived from pituitary corticotroph tumors (Cushing disease) or from non-pituitary tumors (ectopic Cushing's syndrome). Hypercortisolemic features of ectopic Cushing's syndrome are severe, and no definitive treatment for paraneoplastic ACTH excess is available. We aimed to identify subcellular therapeutic targets by elucidating transcriptional regulation of the human ACTH precursor POMC (proopiomelanocortin) and ACTH production in non-pituitary tumor cells and in cell lines derived from patients with ectopic Cushing's syndrome. We show that ectopic hPOMC transcription proceeds independently of pituitary-specific Tpit/Pitx1 and demonstrate a novel E2F1-mediated transcriptional mechanism regulating hPOMC We identify an E2F1 cluster binding to the proximal hPOMC promoter region (-42 to +68), with DNA-binding activity determined by the phosphorylation at Ser-337. hPOMC mRNA expression in cancer cells was upregulated (up to 40-fold) by the co-expression of E2F1 and its heterodimer partner DP1. Direct and indirect inhibitors of E2F1 activity suppressed hPOMC gene expression and ACTH by modifying E2F1 DNA-binding activity in ectopic Cushing's cell lines and primary tumor cells, and also suppressed paraneoplastic ACTH and cortisol levels in xenografted mice. E2F1-mediated hPOMC transcription is a potential target for suppressing ACTH production in ectopic Cushing's syndrome. © 2016 Society for Endocrinology.

  11. Analysis on Heritability of Genetic Traits in Progeny of Peach%桃杂交后代基因性状遗传分析

    Institute of Scientific and Technical Information of China (English)

    韩玉虎; 董冰; 田歌; 王璐; 田建保; 安·尼古接

    2011-01-01

    Heritability of quantitative genetic baits like full bloom date, fruit ripening date, fruit size/ color/ flavor of peach (Prunus persica L.) were analyzed on the base of the records collected during 5 years 1987 to 1991 from 1 361 peach seedlings of 12 crosses including 6 standard, 5 semi-dwarf and 2 dwarf varieties and selections at the Fruit Experiment Institute of Rome, Italy. The heritability of the other 7 traits, such as fruit shape, flesh firmness and color, productivity, flesh adhesion, plant size and fruit quality were studied as well. Full bloom date, fruit ripening date, fruit size, fruit color were moderately heritable traits (H2=0.3 ~ 05 ), while fruit flavor was relatively low heritable. As for fruit shape, round fruit was dominant over elliptic, ovate and oblate ones at a ratio of 76 :12 :8 : 4. Parents in the crosses tended to produce -0.2 (Max. 5) grade less firm fruits averagely in their progenies. Although all the parents had yellow fruit flesh, their progenies segregated broadly into three types of flesh color with an absolute dominance of yellow over white and green fruit flesh. The productivity in progeny were 1 grade lower than the average value of their parents, high cropping seedlings were however obtained in the progeny of the crosses, such as, 7310828 (J.H.Hale x Bonanza) x Bonanza, and Fortyniner x 7310902 (J.H.Hale x Bonanza). Free stone was dominant over semi-free and clingstone except the progeny from the cross of 7310828 x Bonanza. Averagely, fruit quality declined for about 2 (Max. 5) grade in the progenies,,hut very large-fruit selections with bright external color and orange flesh were obtained in the progenies of the crosses Fortyniner × 7310828 and O'Henry(op.). Of all the progenies, standard plant size was significantly dominant over semi-dwarf and dwarf plant (82 : 14:4). Pillar peach could be one of the suitable parents for getting vigorous upright seedlings.%分析了12个桃杂交组合(杂交亲本包括6个普通型、5

  12. Characterization of interspecific hybrids and backcross progenies from a cross between Oryza minuta and Oryza sativa

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Oryza minuta, a tetraploid wild relative of cultivated rice, is an important source for the genetic improvement. Interspecific hybrids were obtained from the cross of O. sativa L. (IR24) and O. minuta (Acc. No. 101133) with 5.58% crossability, which ranged from 0.11% to 1.62% in the backcross generations. The chromosome numbers of the backcross progenies were 24 to 48. Seven yield-related traits of the parents, hybrid F1, and backcross progenies were evaluated. Simple sequence repeat markers analysis showed that the polymorphism ratio of SSR bands between IR24 and Acc. No. 101133 was 93.2%. The average donor segment number, length, donor genome size, and percentage of donor genome of 92 BC3F1 plants (2n=24) were 24.1, 17.8 cM, 438.4 cM and 26.2%, respectively. They were complex variation and uneven among the chromosomes. These introgression lines could be used to identify the favorable genes of O. minuta and provide a new platform for the genetic improvement of cultivated rice.

  13. Comparison of remote consequences in Taraxacum officinale seed progeny collected in radioactively or chemically contaminated areas.

    Science.gov (United States)

    Pozolotina, Vera N; Antonova, Elena V; Bezel, Victor S

    2012-10-01

    We carried out a comparative study of seed progeny taken from the dandelion (Taraxacum officinale s.l.) coenopopulations exposed for a long time to radioactive or chemical contamination originated from the East-Ural radioactive trace zone (EURT) or Nizhniy Tagil metallurgical combine impact zone (NTMC), respectively. Coenopopulations from EURT, NTMC and background areas significantly differ from each other with respect to the qualitative and quantitative composition of allozyme phenes. An analysis of clonal diversity showed the uniqueness of all coenopopulations in terms of their phenogenetics. P-generation seed viability was found to decrease in a similar manner as all types of the industrial stress increased. Studies of F (1)-generation variability in radio- and metal resistance by family analysis showed that seed progeny from EURT impact zone possessed high viability that, however, was accompanied by development of latent injuries resulting in low resistance to additional man-caused impacts. In F (1)-generation originated from NTMC zone, high seed viability was combined with increased resistance to provocative heavy metal and radiation exposure. No significant differences in responses to 'habitual' and 'new' factors, i.e. pre-adaptation effect, were found in samples from the contaminated areas.

  14. Analysis list: E2f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available E2f1 Blood,Liver + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E2f1.1....tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E2f1.5.tsv http://dbarchive.biosciencedbc.jp/kyus...hu-u/mm9/target/E2f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/E2f1.Blood.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/colo/E2f1.Liver.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/colo/Blood.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Liver.gml ...

  15. Analysis of F1F0-ATPase from Helicobacter pylori.

    OpenAIRE

    1997-01-01

    The adaptive mechanisms that permit Helicobacter species to survive within the gastric mucosa are not well understood. The proton-translocating F1F0-ATPase is an important enzyme for regulating intracellular pH or synthesizing ATP in many other enteric bacteria; therefore, we used degenerate primers derived from conserved bacterial F1F0-ATPase sequences to PCR amplify and clone the gene (atpD) encoding the H. pylori F1F0-ATPase beta subunit. The deduced amino acid sequences of the F1F0-ATPase...

  16. Analysis list: POU5F1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available POU5F1 Epidermis,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyu...archive.biosciencedbc.jp/kyushu-u/hg19/target/POU5F1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/POU5F1.Epidermis...U5F1.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Epidermis.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Pluripotent_stem_cell.gml ...

  17. Predição de parâmetros e valores genéticos para caracteres de crescimento e produção de látex em progênies de seringueira Genetic parameters and values prediction for growth and latex production traits in rubber tree progenies

    Directory of Open Access Journals (Sweden)

    Reginaldo Brito da Costa

    2010-01-01

    Full Text Available O trabalho objetivou estimar parâmetros e valores genéticos para os caracteres altura, diâmetro, produção de látex e produtividade de progênies de seringueira. As progênies meio-irmãos foram estabelecidas sob delineamento de blocos ao acaso, com 28 tratamentos (progênies, cinco repetições e dez plantas por parcela. Aos três anos de idade, as progênies foram avaliadas quanto aos caracteres: a altura total (cm; b diâmetro (mm; c Produção de borracha seca (g e produtividade (g cm-2. As estimativas dos coeficientes de herdabilidades individuais (0,33; 0,24 e 0,51 para os caracteres altura, diâmetro e produtividade, respectivamente, foram consideradas expressivas. Para o caráter produção de borracha seca, a estimativa do coeficiente de herdabilidade individual, no sentido restrito (9%, embora de baixa magnitude, revela excelente possibilidade de seleção, pois conduziram a estimativa da herdabilidade, em nível de médias de família, a um valor equivalente a 68%. A acurácia entre os valores genéticos preditos e os verdadeiros foram de 0,80 para altura, 0,76 para diâmetro, 0,62 para produção de borracha seca e 0,86 para produtividade. As estimativas de herdabilidades individuais associadas às de médias de progênies podem maximizar os ganhos genéticos com a seleção na população. A inclusão do caráter produtividade foi promissora e deve ser utilizada na seqüência das avaliações, subsidiando o programa de melhoramento genético da espécie no Estado de Mato Grosso do Sul.This study aimed to estimate genetic values and parameters for plant height, diameter, rubber yield and productivity (g cm-2 of rubber tree progenies. Half-sib progenies were established in a complete randomized block, with 28 treatments, five replicates and 10 plants per plot. At three years of life, the progenies were evaluated regarding the following characters: diameter, rubber production and productivity (g cm². The estimative coefficients

  18. RAPD linkage mapping in a longleaf pine x slash pine F1 family.

    Science.gov (United States)

    Kubisiak, T L; Nelson, C D; Nance, W L; Stine, M

    1995-06-01

    Random amplified polymorphic DNAs (RAPDs) were used to construct linkage maps of the parent of a longleaf pine (Pinus palustris Mill.) slash pine (Pinus elliottii Englm.) F1 family. A total of 247 segregating loci [233 (1∶1), 14 (3∶1)] and 87 polymorphic (between parents), but non-segregating, loci were identified. The 233 loci segregating 1∶1 (testcross configuration) were used to construct parent-specific linkage maps, 132 for the longleaf-pine parent and 101 for the slash-pine parent. The resulting linkage maps consisted of 122 marker loci in 18 groups (three or more loci) and three pairs (1367.5 cM) for longleaf pine, and 91 marker loci in 13 groups and six pairs for slash pine (952.9 cM). Genome size estimates based on two-point linkage data ranged from 2348 to 2392 cM for longleaf pine, and from 2292 to 2372 cM for slash pine. Linkage of 3∶1 loci to testcross loci in each of the parental maps was used to infer further linkages within maps, as well as potentially homologous counterparts between maps. Three of the longleaf-pine linkage groups appear to be potentially homologous counterparts to four different slash-pine linkage groups. The number of heterozygous loci (previously testcross in parents) per F1 individual, ranged from 96 to 130. With the 87 polymorphic, but non-segregating, loci that should also be heterozygous in the F1 progeny, a maximum of 183-217 heterozygous loci could be available for mapping early height growth (EHG) loci and for applying genomic selection in backcross populations.

  19. ARTICLE - Inbreeding depression in castor bean (Ricinus communis L. progenies

    Directory of Open Access Journals (Sweden)

    Milton Krieger

    2012-12-01

    Full Text Available The purpose of this study was to investigate inbreeding depression (DE in castor bean. From a population derived from the Guarani cultivar, 60 mother plants were sampled. Three types of progenies were obtained from each one: from self-pollination (AU, from crosses (CR and from open pollination (PL. Grain yield of the progenies was evaluated in two locations. There was a strong interaction of progenies x locations, which led to obtaining estimates within each location. Broad variation was observed in inbreeding depression, with mean values of 6.7% and 13.4%, comparing AU progenies with PL progenies. It was observed that the population has high potential for selecting promising inbred lines. The frequency of mother plants generating progenies with simultaneous high general combination capacity and low inbreeding depression was low. Recurrent selection will increase the occurrence of parent plants associating these two properties, which is necessary for obtaining superior synthetic varieties.

  20. Progress of the NTSC-F1 primary frequency standard

    Institute of Scientific and Technical Information of China (English)

    RUAN; Jun; WANG; Xinliang; LIU; Dandan; GUAN; Yong; ZHANG; Hui; CHEN; Jiang; LIN; Rui; YU; Fengxiang; SHI; Junru; ZHANG; Shougang

    2015-01-01

    The SI "second"is realized by caesium primary frequency standards( PFSs) using laser cooled atoms in a fountain configuration. Four sub systems and operation procedure of the NTSC-F1 primary frequency standard are introduced in the paper.The frequency stability of NTSC-F1 is 3.0×10-13/ τ-1 / 2compared to hydrogen maser. Four terms of frequency shift and uncertainty including second order Zeeman frequency shift,cold collision shift,gravity shift and blackbody shift are evaluated. The improvement of NTSC-F1 is introduced.

  1. General formulae for f1 -> f2 γ

    Science.gov (United States)

    Lavoura, L.

    2003-07-01

    At one-loop level the decay f_1 to f_2 γ, where f1 and f2 are two spin-1/2 particles with the same electric charge, is mediated by a boson B and a spin-1/2 fermion F. The boson B may have either spin - interacting with the fermions through the Dirac matrices 1 and γ_5 - or spin 1 - with V+ A and V- A couplings to the fermions. I give general formulae for the one-loop electroweak amplitude of f_1 to f_2 γ in all these cases.

  2. General formulae for f1 --> f2 gamma

    CERN Document Server

    Lavoura, L

    2003-01-01

    At one-loop level the decay f1 --> f2 gamma, where f1 and f2 are two spin-1/2 particles with the same electric charge, is mediated by a boson B and a spin-1/2 fermion F. The boson B may have either spin 0 - interacting with the fermions through Dirac matrices 1 and gamma5 - or spin 1 - with V+A and V-A couplings to the fermions. I give general formulae for the one-loop electroweak amplitude of f1 --> f2 gamma in all these cases.

  3. Estimation of human dose to radon progeny

    Energy Technology Data Exchange (ETDEWEB)

    Shimo, Michikuni [Gifu Coll. of Medical Technology, Sekiichi, Gifu (Japan)

    1996-12-01

    The aim of the paper is the estimation of the effective dose due to radon progeny for Japanese population. The estimation was performed by a modified UNSCEAR equation. The equation was needed the radon concentration annual occupancy time and the tidal volume on Japanese people and the dose conversion coefficient are needed. Furthermore, not only these figures but also unattached fraction and aerosol distribution data obtained in Japan and the factor related to the Japanese living style were used in the calculation. We used following figures as representative value in Japan; radon concentration: 13(6 - 25) Bq/m{sup 3} indoors and 6.7(3.5 - 13) Bq/m{sup 3} outdoors; the equilibrium factor: 0.45(0.35 - 0.57) indoors and 0.70(0.50 - 0.90) outdoors; the occupancy factor: 0.87 indoors, 0.09 outdoors and 0.04 in vehicle for male and 0.91 indoors, 0.06 outdoors and 0.03 in vehicle for female; the tidal volume: 7,000 (4,000 - 8,000) m{sup 3} for male, 6,200 (3,500 - 7,500) m{sup 3} for female. The effective doses due to radon progeny were estimated to be 0.45 mSv/y for male and 0.40 mSv/y for female, and the variance was -80 - +130%. These values were 1/2 - 1/3 as small as values shown by UNSCEAR 1993 Report and estimated by ICRP Publication 65. (author)

  4. Induction og 2n gametes for overcoming F1-sterility in lily and tulip

    NARCIS (Netherlands)

    Barba Gonzalez, R.; Miller, C.T.; Ramanna, M.S.; Tuyl, van J.M.

    2006-01-01

    For overcoming F1-sterility in interspecific hybrids, mitotic and meiotic polyploidisation is applied in lily and can result in fertile allopolyploids. The mechanism of viable pollen production of mitotic and meiotic polyploidisation is quite different. Mitotic polyploids are obtained by artificial

  5. 78 FR 69538 - Attestation Process for Employers Using F-1 Students in Off-Campus Work

    Science.gov (United States)

    2013-11-20

    ... workers, Employment, Employment and training, Enforcement, Forest and forest products, Fraud, Health professions, Immigration, Labor, Longshore and harbor work, Migrant workers, Nonimmigrant workers, Passports... employers seeking to hire F-1 foreign students as part-time workers off-campus. These subparts...

  6. Applications of a silicon photodiode detector for radon progeny measurements

    CERN Document Server

    Voytchev, M; Chambaudet, A; Georgiev, G; Iovtchev, M

    1999-01-01

    An application of our developed silicon photodiode detector for radon progeny measurements is presented in this paper. It was determined the deposition velocity for free (3.6+-0.7)x10 sup - sup 3 m s sup - sup 1 and attached (1.0+-0.5)x10 sup - sup 5 m s sup - sup 1 fraction of short living radon progeny.

  7. Recent advances in Progeny testing program for Nili Ravi buffalo in Pakistan

    OpenAIRE

    A. Ghaffar; M.A Khan

    2010-01-01

    Progeny testing program was started in Nili-Ravi buffalo at Livestock Production and Research Institute (LPRI), Bahadurnagar, Okara in 1979-80. Initially buffalo bulls used at Livestock Experiment Station (LES) Qadirabad and Rakh Gulaman during 1964-78 were evaluated. Five out of ten at Rakh Gulaman and 10 out of 22 at Qadirabad bulls were positive. As none of these bulls were available, therefore, sons and grandsons of these bulls were selected for production of future candidate bulls. All t...

  8. Integrating Bioengineered F1 Motors into Nano-Structured Surfaces

    Science.gov (United States)

    2013-04-23

    Cindy Berrie, Fei Gao. Insertion of a Rigid Structural Element into the Regulatory Domain of the Chloroplast F1-ATPase Gamma Subunit for Rotational...Studies., 15th International Photosynthesis Congress. 2010/08/22 01:00:00, . : , 12/27/2011 3.00 . The Mutation E242K in the chloroplast ATP synthase... chloroplast F1-ATPase gamma subunit for rotational studies. Proceedings of the 15th International Congress on Photosynthesis, 2011, pp.123-126. 2. Colvert

  9. RADON PROGENY AS AN EXPERIMENTAL TOOL FOR DOSIMETRY OF NANOAEROSOLS

    Energy Technology Data Exchange (ETDEWEB)

    Ruzer, Lev; Ruzer, Lev S.; Apte, Michael G.

    2008-02-25

    The study of aerosol exposure and dosimetry measurements and related quantitation of health effects are important to the understanding of the consequences of air pollution, and are discussed widely in the scientific literature. During the last 10 years the need to correlate aerosol exposure and biological effects has become especially important due to rapid development of a new, revolutionary industry ?-- nanotechnology. Nanoproduct commerce is predicted to top $1 trillion by 2015. Quantitative assessment of aerosol particle behavior in air and in lung deposition, and dosimetry in different parts of the lung, particularly for nanoaerosols, remains poor despite several decades of study. Direct measurements on humans are still needed in order to validate the hollow cast, animal studies, and lung deposition modeling. We discuss here the use of nanoscale radon decay products as an experimental tool in the study of local deposition and lung dosimetry for nanoaerosols. The issue of the safe use of radon progeny in such measurements is discussed based on a comparison of measured exposure in 3 settings: general population, miners, and in a human experiment conducted at the Paul Scherer Institute (PSI) in Switzerland. One of the properties of radon progeny is that they consist partly of 1 nm radioactive particles called unattached activity; having extremely small size and high diffusion coefficients, these particles can be potentially useful as radioactive tracers in the study of nanometer-sized aerosols. We present a theoretical and experimental study of the correlation between the unattached activity and aerosol particle surface area, together with a description of its calibration and method for measurement of the unattached fraction.

  10. Aneuploid progenies of triploid hybrids between diploid and tetraploid loach Misgurnus anguillicaudatus in China.

    Science.gov (United States)

    Li, Ya-Juan; Gao, Yang-Chun; Zhou, He; Ma, Hai-Yan; Lin, Zhong-Qiao; Ma, Tian-Yu; Sui, Yi; Arai, Katsutoshi

    2016-10-01

    Triploid Chinese loach, Misgurnus anguillicaudatus, hybrids between tetraploids from Hubei Province and diploids from Liaoning Province were mated with either diploid wild-type or triploid hybrids to analyze viability and ploidy of the resultant progenies. Both triploid males and females generated fertile gametes, but progenies from the crosses using gametes of triploid hybrids did not survive beyond the larval stages. In crosses between wild-type diploid females and triploid hybrid males, embryos ranging from 2.2n to 2.6n were predominant with a mode of either 2.4n (chromosome numbers 59, 60, 61) or 2.5n (chromosome numbers 62, 63). Those from the crosses between triploid hybrid females and diploid males gave a modal ploidy level at approximately 2.5n in one case, but a shift to a higher ploidy level was observed in other embryos. In the progenies between triploid hybrid females and males, the ploidy level at approximately 3.0n (chromosome numbers 74, 75, 76) was most frequent. The cytogenetic results of the progenies suggest the production of aneuploid gametes with a modal ploidy level at approximately 1.5n in triploid hybrids. However, a shift to higher chromosome numbers in gametes was observed in certain cases, suggesting the involvement of mortality selection of gametes and/or zygotes with lower chromosome numbers.

  11. Degree of inheritance and heritability of yield in parental genotypes and F1 hybrids of tobacco

    Directory of Open Access Journals (Sweden)

    Aleksoski Jane A.

    2011-01-01

    Full Text Available The mode and level of inheritance of green and dry mass yield per stalk were investigated in four parental genotypes (Burley - B 2/93, Suchum - S1, Suchum - S2 and Prilep - P-84 and in their six diallel F1 hybrids. The trial was set up in 2007, 2008 and 2009 in the field of Tobacco Institute-Prilep in a randomized block design with four replications. The aim of the investigation was to estimate the heritability as an indicator of the inheritance of the yield as one of the most important quantitative characters of tobacco, in order to give suggestions for the selection of parental genotypes and directions for the creation of new varieties. The mode of inheritance was estimated according to the test - significance of the mean value of F1 progeny compared to the parental average. Narrow-sense heritability was estimated after Allard (1960, while broad-sense heritability and genetic components were estimated after Mather and Jinks (1974. The mode of inheritance in the hybrids was different. Positive heterosis for green and dry mass yields per stalk was recorded in S1 x S2. Negative heterosis for green mass yields per stalk was recorded in S1 x P-84 and S2 x P-84, while for dry mass yield it was recorded in S1 x P-84. Inheritance of the characters during the three years of investigation was identical. The higher heritability index of both types was recorded for dry mass yield. As regards inheritance of the yield, the values of broad-sense heritability were higher than those of narrow-sense heritability.

  12. Comparative Study on Meat Production between Dorset Sheep and Lamb of Small-tail Han Fl, Suffolk Sheep and Lamb of Small-tail Han F1 and Lamb of Small -tail Han Sheep%陶赛特、萨福克与小尾寒羊杂交产肉性能的改良效果

    Institute of Scientific and Technical Information of China (English)

    孙俊峰

    2010-01-01

    以萨福克羊、陶赛特羊为父本与小尾寒羊进行杂交,在同等饲养管理条件下,其萨寒F1、陶寒F1与小尾寒羊间在90 d育肥期内日增重差异极显著(P0.05).实验表明:萨寒F1、陶寒F1生长速度快、产肉性能高的优点已在其杂交后代身上体现出来,杂种优势显著.

  13. Evaluation of Antioxidant Compounds and Total Sugar Content in a Nectarine [Prunus persica (L. Batsch] Progeny

    Directory of Open Access Journals (Sweden)

    Yolanda Gogorcena

    2011-10-01

    Full Text Available Epidemiological studies suggest that consumption of fruit rich in phenolic compounds is associated with health-protective effects due to their antioxidant properties. For these reasons quality evaluation has become an important issue in fruit industry and in breeding programs. Phytochemical traits such as total phenolics, flavonoids, anthocyanins, L-ascorbic acid, sugar content and relative antioxidant capacity (RAC were analyzed over four years in flesh fruit of an F1 population “Venus” × “Big Top” nectarines. Other traits such as harvesting date, yield, fruit weight, firmness, soluble solids concentration (SSC, pH, titratable acidity (TA and ripening index (RI were also determined in the progeny. Results showed high variability among genotypes for all analyzed traits. Total phenolics and flavonoids showed significant positive correlations with RAC implying that both are important antioxidant bioactive compounds in peaches. We found genotypes with enhanced antioxidant capacity and a better performance than progenitors, and in consequence the best marketability.

  14. Dosimetry of radium-223 and progeny

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, D.R. [Pacific Northwest National Lab., Richland, WA (United States); Sgouros, G. [Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    1999-01-01

    Radium-223 is a short-lived (11.4 d) alpha emitter with potential applications in radioimmunotherapy of cancer. Radium-223 can be complexed and linked to protein delivery molecules for specific tumor-cell targeting. It decays through a cascade of short-lived alpha- and beta-emitting daughters with emission of about 28 MeV of energy through complete decay. The first three alpha particles are essentially instantaneous. Photons associated with Ra-223 and progeny provide the means for tumor and normal-organ imaging and dosimetry. Two beta particles provide additional therapeutic value. Radium-223 may be produced economically and in sufficient amounts for widescale application. Many aspects of the chemistry of carrier-free isotope preparation, complexation, and linkage to the antibody have been developed and are being tested. The radiation dosimetry of a Ra-223-labeled antibody shows favorable tumor to normal tissue dose ratios for therapy. The 11.4-d half-life of Ra-223 allows sufficient time for immunoconjugate preparation, administration, and tumor localization by carrier antibodies before significant radiological decay takes place. If 0.01 percent of a 37 MBq (1 mCi) injection deposits in a one gram tumor mass, and if the activity is retained with a typical effective half-time (75 h), the absorbed dose will be 163 mGy MBq{sup {minus}1} (600 rad mCi{sup {minus}1}) administered. 49 refs., 5 figs., 2 tabs.

  15. Analysis of the meiosis in the F1 hybrids of Longiflorum x Asiatic(LA) of lilies (Lilium) using genomic in situ hybridization

    Institute of Scientific and Technical Information of China (English)

    Shujun Zhou; Munikote S. Ramanna; Richard G.F.Visser; Jaap M. van Tuyl

    2008-01-01

    Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaeeae are two important groups of modern lily eultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups have been obtained. However, the F1hybrids are highly sterile or some of them could produce a small number of 2n gametes, and their BC1 progenies are usually triploids. Dutch lily breeders have selected many cultivars from these BC1 progenies based on their variation. It is presumably suggested that such variation could be caused by intergenomic recombination and abnormal meiosis during gamete formation in F1 hybrids of Longiflorum x Asiatic (LA) hybrids in Lilium. Therefore, the meiotic process of ten F1 LA hybrids was cytologically investigated using genomic in situ hybridization and traditional cytological methods in the present research.The results showed that: at metaphase I, the homoeologous chromosome pairing among different F1hybrids ranged from 2.0 to 11.4 bi-valents formed by homoeologous chromosomes per pollen mother cell (PMC), and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all biva-lents were disjoined and most univalents were divided. Both the disjoined bivalents (half-bivalents) and the divided univalents (sister chromatids) moved to the opposite poles, and then formed two groups of chromosomes; because the two resulting half-bivalents retained their axes in the cell undisturbed, many crossover types, including single crossovers, three strand double crossovers, four strand double crossovers, four strand triple crossovers, and four strand multiple crossovers between the non-sister chromatids in the tetrads of bivalents,were clearly inferred by analyzing the breakpoints on the disjoined bivalents. The present investigation not only

  16. F1 hybrids of BALB/c and C57BL/6 mouse strains respond differently to low-dose ionizing radiation exposure

    Indian Academy of Sciences (India)

    Sanjay Mukherjee; K. B. Sainis; Deepti D. Deobagkar

    2014-12-01

    There are evidences to show that response to ionizing radiations have genetic influence. To investigate this further, reciprocal F1 hybrids were genereted by crossbreeding the radiation-susceptible BALB/c mouse strain with resistant C57BL/6 in a sex-specific manner (BALB/c♂×C57BL/6♀ = B6BcF1; C57BL/6♂× BALB/c♀ =BcB6F1). These hybrids were compared with each other and to the parental strains with respect to transcriptional responses to low-dose ionizing radiation exposure (LDIR). The two F1 hybrids showed drastic differences in their gene expression profiles to ionizing radiation exposure particularly in case of the genes involved in DNA damage response and repair process. Also, the inheritance pattern of the gene expression was found to be complex and could not be explained solely on the basis of parental expression pattern. It was concluded that there is a differential transmission of susceptible trait alleles from the parents to F1 progeny which is dependent on the sex of the parent mouse strain used to set up the crosses and other environmental factors.

  17. Melhoramento do cafeeiro: XII - Variabilidade em linhas puras de café Coffee breeding: XII - Variability within isogenic coffee progenies

    Directory of Open Access Journals (Sweden)

    H. Antunes Filho

    1957-01-01

    1934 apresentou menor quantidade de sementes moca do que as linhas puras e significativamente menos que a progênie 357-21 Dp. Quanto à variabilidade, a do híbrido se mostrou intermediária, aproximando-se da linha pura com variância maior. As variâncias das outras progênies estudadas são bem mais elevadas, notando-se tendência de aumento com as autofecundações sucessivas. Com relação às sementes concha, a porcentagem média para o híbrido não difere das linhas puras e o valor da variância é intermediário. Para as demais progênies analisadas os valores das variâncias são maiores e mostram tendência de redução com as autofecundações. O valor da peneira média correspondente ao tamanho das sementes não difere para as três linhas isogênicas, a variabilidade sendo, porém, menor para o híbrido. Para as outras progênies não se notou redução do tamanho como efeito da autofecundação e as suas variâncias não mostram tendência de aumento ou redução nas gerações estudadas. Dos itens analisados, destacam-se como mais promissores do ponto de vista da produção e altura das plantas (a qual pode representar o vigor vegetativo a progénie de Bourbon Vermelho 959 e a de Bourbon Amarelo J 24ex. Dependendo do característico analisado, notou-se que a variância entre plantas nos canteiros do híbrido F1 pode ser igual, intermediária ou menor do que a das linhas puras. Em nenhum caso, porém, a variância do híbrido se mostrou maior que a das linhas puras.Three isogenic progenies of Red Bourbon coffee (Coffea arabica L.var. bourbon (B. Rodr. Choussy were investigated in relation to several characteristics in a balanced lattice experiment with 16 entries, five replications, each plot with four individual plants. Two of these isogenic progenies were obtained by artificial self-pollination of two plants which were derived from haploid sporophytes after their chromosomes were duplicated (357-21 and RP 13. The third isogenic progeny is the F1 of

  18. Melhoramento do cafeeiro XV: variabilidade observada em progênies de café Coffee breeding XV: variability observed in coffee progenies

    Directory of Open Access Journals (Sweden)

    A. Carvalho

    1959-01-01

    Full Text Available As análises das produções de cafeeiros constituintes de diversas progênies, principalmente de café Bourbon Vermelho, com produções controladas por 12 a 15 anos. mostraram acentuada variabilidade na produção, o mesmo ocorrendo entre cafeeiros F1 derivados de hibridações entre plantas selecionadas. A fim de avaliar as causas dessa variação foram escolhidas, em quatro progenies S1 e em três híbridos F1, cafeeiros com as mais altas e as mais baixas produções, bem como uma planta em cada progênie com produção correspondente à classe mediana, a fim de servirem de plantas matrizes para derivação de progênies correspondentes às gerações S2 e F2. Estas foram plantadas, em 1953, em um ensaio comparativo, onde também foram incluídas novas progenies S1 e F1 dos mesmos cafeeiros, bem como progênies selecionadas de outras variedades comerciais como Bourbon Amarelo e Mundo Novo. A análise dos resultados das quatro primeiras produções (1955 a 1958 bem como da altura e vigar dos cafeeiros indicou, em todos os conjuntos de progênies examinadas, que a grande variação observada entre as plantas de uma mesma progênie de Bourbon é principalmente devida a influências do ambiente, desde que não se notaram diferenças na produção das progênies S2 e F2 derivadas de plantas S1 e F1 com as mais altas e as mais baixas produções. A mesma condição se verificou em relação à altura das plantas. Os valores das variâncias da produção para as progênies S1 e S2 em cada grupo de progenies não indicaram nenhum efeito específico da autofecundação, reduzindo-os ou aumentando-os. Nas gerações F2 as variâncias se mostraram mais uniformes, porém as diferenças entre as variâncias das gerações F1 e F2 não foram significativas. Pode-se concluir dos dados dêste ensaio que a seleção do café Bourbon Vermelho deve ser baseada na produção média das progenies e que a seleção dentro da progênie não conduz a nenhum

  19. Genetic Variation Among Open-Pollinated Progeny of Eastern Cottonwood

    Science.gov (United States)

    R. E. Farmer

    1970-01-01

    Improvement programs in eastern cottonwood (Populus deltoides Bartr.) are most frequently designed to produce genetically superior clones for direct commercial use. This paper describes a progeny test to assess genetic variability on which selection might be based.

  20. f(1)、f(0)、f(-1)表示f(x)=ax2+bx+c解竞赛题

    Institute of Scientific and Technical Information of China (English)

    严启国; 陈纯亮

    2003-01-01

    @@ 设函数f(x)=ax2+bx+c(-1≤x≤1),则f(1)=a+b+c,f(0)=c,f(-1)=a-b+c,解得a=1/2f(1)+1/2f(-1)-f(0),b=1/2f(1)-1/2f(-1),c=f(0),从而有f(x)=[1/2f(1)+1/2f(-1)-f(0)]x2+[1/2f(1)-1/2f(-1)]x+f(0),利用这一表示形式可以解下列竞赛题.

  1. Multivariate and Clustering Analysis in Sweet Tamarind Seedling Progenies

    OpenAIRE

    D. Saraladevi, V. Ponnuswami, R.M. Vijayakumar and S.Chitra

    2010-01-01

    Seventy eight seedling progenies of a sweet tamarind type collected from Dindigul region were planted at HorticulturalCollege and Research Institute, Periyakulam during September 2005. These seedling progenies in pre bearing stagewere evaluated to assess the variability existing in respect of growth parameters viz., tree height, girth and canopyspread. Among the three characters studied, canopy spread exhibited highest correlation with girth (0.61) than treeheight (0.21). Tree height having h...

  2. M2-F1 on lakebed with pilot Milt Thompson

    Science.gov (United States)

    1963-01-01

    NASA Flight Research Pilot Milt Thompson, shown here on the lakebed with the M2-F1 lifting body, was an early backer of R. Dale Reed's lifting-body proposal. He urged Flight Research Center director Paul Bikle to approve the M2-F1's construction. Thompson also made the first glide flights in both the M2-F1 and its successor, the heavyweight M2-F2. The wingless, lifting body aircraft design was initially conceived as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, NASA Flight Research Center (later Dryden Flight Research Center, Edwards, CA) management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and racing slicks, the Pontiac could tow the 1,000-pound M2-F1 110 miles per hour in 30 seconds. It proved

  3. Robustness of the rotary catalysis mechanism of F1-ATPase.

    Science.gov (United States)

    Watanabe, Rikiya; Matsukage, Yuki; Yukawa, Ayako; Tabata, Kazuhito V; Noji, Hiroyuki

    2014-07-11

    F1-ATPase (F1) is the rotary motor protein fueled by ATP hydrolysis. Previous studies have suggested that three charged residues are indispensable for catalysis of F1 as follows: the P-loop lysine in the phosphate-binding loop, GXXXXGK(T/S); a glutamic acid that activates water molecules for nucleophilic attack on the γ-phosphate of ATP (general base); and an arginine directly contacting the γ-phosphate (arginine finger). These residues are well conserved among P-loop NTPases. In this study, we investigated the role of these charged residues in catalysis and torque generation by analyzing alanine-substituted mutants in the single-molecule rotation assay. Surprisingly, all mutants continuously drove rotary motion, even though the rotational velocity was at least 100,000 times slower than that of wild type. Thus, although these charged residues contribute to highly efficient catalysis, they are not indispensable to chemo-mechanical energy coupling, and the rotary catalysis mechanism of F1 is far more robust than previously thought.

  4. Supercongruences satisfied by coefficients of 2F1 hypergeometric series

    CERN Document Server

    Chan, Heng Huat; Krattenthaler, Christian; Osburn, Robert

    2009-01-01

    Recently, Chan, Cooper and Sica conjectured two congruences for coefficients of classical 2F1 hypergeometric series which also arise from power series expansions of modular forms in terms of modular functions. We prove these two congruences using combinatorial properties of the coefficients.

  5. The transcription factor E4F1 coordinates CHK1-dependent checkpoint and mitochondrial functions.

    Science.gov (United States)

    Rodier, Geneviève; Kirsh, Olivier; Baraibar, Martín; Houlès, Thibault; Lacroix, Matthieu; Delpech, Hélène; Hatchi, Elodie; Arnould, Stéphanie; Severac, Dany; Dubois, Emeric; Caramel, Julie; Julien, Eric; Friguet, Bertrand; Le Cam, Laurent; Sardet, Claude

    2015-04-14

    Recent data support the notion that a group of key transcriptional regulators involved in tumorigenesis, including MYC, p53, E2F1, and BMI1, share an intriguing capacity to simultaneously regulate metabolism and cell cycle. Here, we show that another factor, the multifunctional protein E4F1, directly controls genes involved in mitochondria functions and cell-cycle checkpoints, including Chek1, a major component of the DNA damage response. Coordination of these cellular functions by E4F1 appears essential for the survival of p53-deficient transformed cells. Acute inactivation of E4F1 in these cells results in CHK1-dependent checkpoint deficiency and multiple mitochondrial dysfunctions that lead to increased ROS production, energy stress, and inhibition of de novo pyrimidine synthesis. This deadly cocktail leads to the accumulation of uncompensated oxidative damage to proteins and extensive DNA damage, ending in cell death. This supports the rationale of therapeutic strategies simultaneously targeting mitochondria and CHK1 for selective killing of p53-deficient cancer cells.

  6. The Transcription Factor E4F1 Coordinates CHK1-Dependent Checkpoint and Mitochondrial Functions

    Directory of Open Access Journals (Sweden)

    Geneviève Rodier

    2015-04-01

    Full Text Available Recent data support the notion that a group of key transcriptional regulators involved in tumorigenesis, including MYC, p53, E2F1, and BMI1, share an intriguing capacity to simultaneously regulate metabolism and cell cycle. Here, we show that another factor, the multifunctional protein E4F1, directly controls genes involved in mitochondria functions and cell-cycle checkpoints, including Chek1, a major component of the DNA damage response. Coordination of these cellular functions by E4F1 appears essential for the survival of p53-deficient transformed cells. Acute inactivation of E4F1 in these cells results in CHK1-dependent checkpoint deficiency and multiple mitochondrial dysfunctions that lead to increased ROS production, energy stress, and inhibition of de novo pyrimidine synthesis. This deadly cocktail leads to the accumulation of uncompensated oxidative damage to proteins and extensive DNA damage, ending in cell death. This supports the rationale of therapeutic strategies simultaneously targeting mitochondria and CHK1 for selective killing of p53-deficient cancer cells.

  7. Assessing hybrid sterility in Oryza glaberrima x O. sativa hybrid progenies by PCR marker analysis and crossing with wide compatibility varieties.

    Science.gov (United States)

    Heuer, Sigrid; Miézan, Kouamé M

    2003-09-01

    Interspecific crossing of the African indigenous rice Oryza glaberrima with Oryza sativa cultivars is hindered by crossing barriers causing 100% spikelet sterility in F(1) hybrids. Since hybrids are partially female fertile, fertility can be restored by back crossing (BC) to a recurrent male parent. Distinct genetic models on spikelet sterility have been developed predicting, e.g., the existence of a gamete eliminator and/or a pollen killer. Linkage of sterility to the waxy starch synthase gene and the chromogen gene C, both located on chromosome 6, have been demonstrated. We selected a segregating BC(2)F(3) population of semi-sterile O. glaberrima x O. sativa indica hybrid progenies for analyses with PCR markers located at the respective chromosome-6 region. These analyses revealed that semi-sterile plants were heterozygous for a marker (OSR25) located in the waxy promoter, whereas fertile progenies were homozygous for the O. glaberrima allele. Adjacent markers showed no linkage to spikelet sterility. Semi-sterility of hybrid progenies was maintained at least until the F(4) progeny generation, suggesting the existence of a pollen killer in this plant material. Monitoring of reproductive plant development showed that spikelet sterility was at least partially due to an arrest of pollen development at the microspore stage. In order to address the question whether genes responsible for F(1) sterility in intraspecific hybrids ( O. sativa indica x japonica) also cause spikelet sterility in interspecific hybrids, crossings with wide compatibility varieties (WCV) were performed. WCV accessions possess "neutral" S-loci ( S(n)) improving fertility in intraspecific hybrids. This experiment showed that the tested S(n)-loci had no fertility restoring effect in F(1) interspecific hybrids. Pollen development was completely arrested at the microspore stage and grains were never obtained after selfing. This suggests that distinct or additional S-loci are responsible for sterility

  8. Genetic Analysis of Heterosis for Number of Spikelets per Panicle and Panicle Length of F1 Hybrids in japonica Rice Hybrids

    Institute of Scientific and Technical Information of China (English)

    ZHU Xu-dong; HONG De-Lin; SHEN Ying; LENG Yan; Henri ADREIT; Julien FROUIN; Didier HARREAU

    2004-01-01

    Genetic analysis of heterosis for number of spikelets per panicle and panicle length of F1 hybrid was conducted by using japonica rice varieties Bing 8979, C Bao, their F1, F2 and triple test cross (TTC) progenies. The two traits, panicle length and number of spikelets per panicle were controlled by polygenes, which were dispersed in the two parents. The dispersion of these polygenes was the genetic basis for the heterosis. Genetic variation in panicle length was mainly due to additive and dominant effects, and the dominant component played a determinative role. For number of spikelets per panicle, the effect of non-allelic genes was highly significant (1% probability level), and there existed epistasis including effects of additive × additive, additive×dominant, and dominant × dominant.

  9. Internal steel structure of M2-F1

    Science.gov (United States)

    1963-01-01

    The internal steel structure for the M2-F1 was built at the Flight Research Center (predecessor of the Dryden Flight Research Center, Edwards, CA) in a section of the calibration hangar dubbed 'Wright Bicycle Shop.' Visible are the stick, rudder pedals, and ejection seat. The external wooden shell was attached to the steel structure. The wingless, lifting body aircraft design was initially conceived as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, Dryden management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and racing slicks, the Pontiac could tow the 1,000-pound M2-F1 110 miles per hour in 30 seconds. It proved adequate for the roughly 400 car tows that got the M2-F1 airborne to prove it could fly

  10. [Study of the effects of gamma-irradiation of common wheat F1 seeds using gliadins as genetic markers].

    Science.gov (United States)

    Kozub, N O; Sozinov, I O; Blium, Ia B; Sozinov, O O

    2013-01-01

    Effects of irradiation of dry F1 seeds with gamma rays in the dose of 200 Gy were studied. Hybrids between near-isogenic lines on the basis of the variety Bezostaya 1 served as the material of investigation. Irradiation markedly reduced productivity traits of F1 plants and did not affect the survival of F1 plants under the given growth conditions. A significant relative increase in the frequency of pollen grains with the 1BL/1RS translocation that formed F2 seeds in comparison with the control was one of the effects of irradiation of F1 seeds. Irradiation with gamma-rays induced mutations at gliadin loci with the frequency of 7,4 % (at 0,5 % in the control).

  11. Assessment of radon and its progeny concentration in Brazilian underground mines

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Talita O.; Rocha, Zildete; Araujo, Gabriela B.D. de, E-mail: talitaolsantos@yahoo.com.br, E-mail: rochaz@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Gouvea, Vandir A.; Cruz, Paulo; Siqueira, Joao B., E-mail: vgouvea@cnen.gov.br, E-mail: pcruz@cnen.gov.br, E-mail: jbsiquei@cnen.gov.br [Comissao Nacional de Energia Nuclear (CNEN-RJ), Rio de Janeiro, RJ (Brazil); Oliveira, Arno H., E-mail: arnoheeren@gmail.com [Universidade Federal de Minas Gerais (DEN/UFMG), Belo Horizonte, MG (Brazil). Departamento de Engenharia Nuclear

    2013-07-01

    Rock, soil and water contain {sup 238}U, {sup 232}Th and their decay products. The distribution of these radionuclides differs in terms of activity concentration depending on the mineral type and origin. All ore processing releases long and short half-life radionuclides, mainly radon and its progeny. It is important to monitor this gas and its products in underground mines in order to assess the radiological hazards of the exposed workers. On this concern, the present work outlines the characterization of the radon and its progeny concentration in the brazilian underground mines. The radon concentration was measured by using E-PERM Electrets Ion Chamber, AlphaGUARD and CR-39 track etch detectors. The radon progeny was determined by using DOSEman detector. The equilibrium state between radon and its progeny was calculated. Based on these data, the annual effective dose for miners was estimated according to UNSCEAR 2000 methodology. Moreover, the contribution from the main sources to the radon level inside mines was evaluated. For this, the following detectors were used: concentration measurements of the soil gas radon were carried out by AlphaGUARD detector; {sup 226}Ra ({sup 214}Bi) specific activity in ore and soil samples were determined by gamma-ray spectrometry HPGe detector; and radon concentration in groundwater samples was performed by RAD7 detector. The radon concentration ranged from 113 to 8171 Bq.m{sup -3} and the Equilibrium Equivalent Concentration varied from 76 to 1174 Bq.m{sup -3}. The general measurement results and the estimated effective doses will be used as bases to prepare the brazilian regulatory standards. (author)

  12. Assessment of dose due to exposure to indoor radon and thoron progeny

    Directory of Open Access Journals (Sweden)

    Prasad Ganesh

    2010-01-01

    Full Text Available The components of the effective dose through inhalation from radon and its progeny are important for human health since they contribute to more than 50% of the total radiation dose from natural sources. As a consequence, radon has been identified as the second leading cause of lung cancer after smoking. Radon and its short lived decay products (218Po, 214Pb, 214Bi, 214Po present in dwellings are a radiation hazard, particularly if such sources are concentrated in the enclosed areas like poorly ventilated houses and underground mines. The indoor radon, thoron, and progeny concentrations were measured in a small hilly town of Budhakedar and the surrounding area of Tehri Garhwal, India, by using LR-115 Type II plastic track detector in a twin cup radon dosimeter. The concentrations of radon progeny were measured as the highest in winter and the lowest in summer while the thoron progeny concentration was found maximum in rainy season and minimum in autumn. The annual exposure to the potential alpha energy of radon and thoron were found to vary from 0.04 WLM to 0.69 WLM with an average value of 0.29 WLM, and 0.03 WLM to 0.37 WLM with an aver- age value of 0.16 WLM, respectively. The annual effective dose due to the exposure to indoor radon and progeny in Budhakedar homes was found to vary from 0.16 mSv to 2.72 mSv with an average value of 1.14 mSv and the effective dose due to the exposure to thoron and progeny was found to vary from 0.18 mSv to 2.49 mSv with an average value of 1.05 mSv. The results of systematic study have been obtained by considering the room as a space in which the radon and thoron levels are directly related to the dynamic and static parameters.

  13. Identification of cocoa trees combining high yield potential and resistance to diseases in segregating progenies In Ecuador

    Science.gov (United States)

    Diseases and low yielding planting material are the main factors limiting production of “fine” or “flavour” cocoa in Ecuador. This makes it necessary to develop modern varieties capable of overcoming these limitations. During the 1960s and 1970s INIAP tested several progenies from selected crosses...

  14. F1FO ATPase vesicle preparation and technique for performing patch clamp recordings of submitochondrial vesicle membranes.

    Science.gov (United States)

    Sacchetti, Silvio; Alavian, Kambiz N; Lazrove, Emma; Jonas, Elizabeth A

    2013-05-04

    Mitochondria are involved in many important cellular functions including metabolism, survival(1), development and, calcium signaling(2). Two of the most important mitochondrial functions are related to the efficient production of ATP, the energy currency of the cell, by oxidative phosphorylation, and the mediation of signals for programmed cell death(3). The enzyme primarily responsible for the production of ATP is the F1FO-ATP synthase, also called ATP synthase(4-5). In recent years, the role of mitochondria in apoptotic and necrotic cell death has received considerable attention. In apoptotic cell death, BCL-2 family proteins such as Bax enter the mitochondrial outer membrane, oligomerize and permeabilize the outer membrane, releasing pro-apoptotic factors into the cytosol(6). In classic necrotic cell death, such as that produced by ischemia or excitotoxicity in neurons, a large, poorly regulated increase in matrix calcium contributes to the opening of an inner membrane pore, the mitochondrial permeability transition pore or mPTP. This depolarizes the inner membrane and causes osmotic shifts, contributing to outer membrane rupture, release of pro-apoptotic factors, and metabolic dysfunction. Many proteins including Bcl-xL(7) interact with F1FO ATP synthase, modulating its function. Bcl-xL interacts directly with the beta subunit of F1FO ATP synthase, and this interaction decreases a leak conductance within the F1FOATPasecomplex, increasing the net transport of H+ by F1FO during F1FO ATPase activity(8) and thereby increasing mitochondrial efficiency. To study the activity and modulation of the ATP synthase, we isolated from rodent brain submitochondrial vesicles (SMVs) containing F1FO ATPase. The SMVs retain the structural and functional integrity of the F1FO ATPase as shown in Alavian et al. Here, we describe a method that we have used successfully for the isolation of SMVs from rat brain and we delineate the patch clamp technique to analyze channel activity (ion

  15. Heterogeneity of linalool chemotypes of Lippia alba (Mill. N.E.Br., based on clonal half-sib progenies

    Directory of Open Access Journals (Sweden)

    Marcos Ribeiro Bottignon

    2011-08-01

    Full Text Available Lippia alba (Mill. N.E.Br. is an aromatic and medicinal shrub native to the American continent. Despite its potential as a source of essential oil for the pharmaceutical and cosmetics industries, few selection and genetic improvement studies have been carried out. The aim of this study was to provide genetic information on this species for breeding programs, showing its selection potential, by investigating clonal half-sib progenies. The following characteristics were evaluated per plant: leaf dry mass (LDM, total dry mass (TDM, leaf yield (LY, essential oil yield (EOY and oil production (OP. Estimates were made for the several genetic parameters including absolute genetic gain at 30% selection intensity, correlations and relative contribution of additive and environmental effects to phenotypic correlation. Two experimental trials on 30 progenies were conducted: one in Campinas, state of São Paulo (SP, Brazil, with two harvests of the aerial part, and one in Monte Alegre do Sul, SP, Brazil, with only one harvest. The trials were conducted in a randomized block design consisting of subplots with three replications, each plot (progeny consisting of 8 to 15 clonally-replicated plants with subplot harvesting. Variations were detected between progenies and harvests, as well as progeny/harvest interactions in the split plot experiment. High heritability and genetic gains were obtained at both sites for LDM, TDM and OP. The lowest variations among progenies were obtained for LY and EOY, highlighting selection problems. Negative additive genetic correlations were obtained for EOY × LDM, EOY × TDM, LY × TDM and LY × LDM. Selection for LDM resulted in increased oil production per plant (OP, even where there was a negative correlation between LDM × EOY.

  16. The Appell function F1 and Regge string scattering amplitudes

    Directory of Open Access Journals (Sweden)

    Jen-Chi Lee

    2014-12-01

    Full Text Available We show that each 26D open bosonic Regge string scattering amplitude (RSSA can be expressed in terms of one single Appell function F1 in the Regge limit. This result enables us to derive infinite number of recurrence relations among RSSA at arbitrary mass levels, which are conjectured to be related to the known SL(5,C dynamical symmetry of F1. In addition, we show that these recurrence relations in the Regge limit can be systematically solved so that all RSSA can be expressed in terms of one amplitude. All these results are dual to high energy symmetries of fixed angle string scattering amplitudes discovered previously [4–8].

  17. Recent advances in Progeny testing program for Nili Ravi buffalo in Pakistan

    Directory of Open Access Journals (Sweden)

    A. Ghaffar

    2010-02-01

    Full Text Available Progeny testing program was started in Nili-Ravi buffalo at Livestock Production and Research Institute (LPRI, Bahadurnagar, Okara in 1979-80. Initially buffalo bulls used at Livestock Experiment Station (LES Qadirabad and Rakh Gulaman during 1964-78 were evaluated. Five out of ten at Rakh Gulaman and 10 out of 22 at Qadirabad bulls were positive. As none of these bulls were available, therefore, sons and grandsons of these bulls were selected for production of future candidate bulls. All the buffaloes at LPRI were evaluat`ed on Most Probable Producing Ability. The elite herd thus formed was used for production for future candidate bulls. Since then a continuous program is in operation at Govt. Livestock Farms and expanded to 27 field sub-centers involving private farmers in four districts Okara, Sahiwal, Faisalabad and Pakpattan. Since the initiation of this program in 1980’s, about 174 bulls have been put under progeny testing program and 137 bulls were evaluated by Daughter-Dam Comparison, out of which sixty eight buffalo bulls contributed positively in milk production of daughters. It is worth mentioning here that milk production of registered buffalo was recorded on monthly intervals in the field along with other necessary reproduction data required to compute the different traits of economic importance.Progeny testing program was started in Nili-Ravi buffalo at Livestock Production and Research Institute (LPRI, Bahadurnagar, Okara in 1979-80. Initially buffalo bulls used at Livestock Experiment Station (LES Qadirabad and Rakh Gulaman during 1964-78 were evaluated. Five out of ten at Rakh Gulaman and 10 out of 22 at Qadirabad bulls were positive. As none of these bulls were available, therefore, sons and grandsons of these bulls were selected for production of future candidate bulls. All the buffaloes at LPRI were evaluat`ed on Most Probable Producing Ability. The elite herd thus formed was used for production for future candidate bulls

  18. Rotation and structure of FoF1-ATP synthase.

    Science.gov (United States)

    Okuno, Daichi; Iino, Ryota; Noji, Hiroyuki

    2011-06-01

    F(o)F(1)-ATP synthase is one of the most ubiquitous enzymes; it is found widely in the biological world, including the plasma membrane of bacteria, inner membrane of mitochondria and thylakoid membrane of chloroplasts. However, this enzyme has a unique mechanism of action: it is composed of two mechanical rotary motors, each driven by ATP hydrolysis or proton flux down the membrane potential of protons. The two molecular motors interconvert the chemical energy of ATP hydrolysis and proton electrochemical potential via the mechanical rotation of the rotary shaft. This unique energy transmission mechanism is not found in other biological systems. Although there are other similar man-made systems like hydroelectric generators, F(o)F(1)-ATP synthase operates on the nanometre scale and works with extremely high efficiency. Therefore, this enzyme has attracted significant attention in a wide variety of fields from bioenergetics and biophysics to chemistry, physics and nanoscience. This review summarizes the latest findings about the two motors of F(o)F(1)-ATP synthase as well as a brief historical background.

  19. Mortality of western corn rootworm larvae on MIR604 transgenic maize roots: field survivorship has no impact on survivorship of F1 progeny on MIR604

    Science.gov (United States)

    Mortality of western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae due to MIR604 transgenic corn (Zea mays L.) expressing the modified Cry3A (mCry3A) protein relative to survivorship on corn with the same genetic background without the gene (isoline corn) was evaluated at three Misso...

  20. Transfer of serum and cells from Yersinia ruckeri vaccinated double-haploid Hot Creek Rainbow trout into outcross F1 progeny elucidates mechanisms of vaccine-induced protection

    Science.gov (United States)

    Yersinia ruckeri is a well-established bacterial pathogen for many salmonid species, against which a formalin-killed bacterin vaccine has been effective in reducing disease outbreaks. Previous studies have reported conflicting results about the protective value of the circulating humoral response to...

  1. Measurements of thoron and radon progeny concentrations in Beijing, China

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Lei; Liu Cuihong; Guo Qiuju [State Key Laboratory of Nuclear Physics and Technology, School of Physics, Peking University, Beijing 100871 (China)], E-mail: qjguo@pku.edu.cn

    2008-12-15

    It has been reported that thoron levels in China are above the world average and may therefore make a significant contribution to the natural background radiation dose. We therefore conducted a pilot study of concentrations of both thoron and radon progeny during the spring of 2006 in the Beijing area, China. A new type of portable 24 h integrating monitor with a CR-39 detector was used during the survey. Seventy dwellings and eight outdoor sites were measured during the survey. For country houses built of red bricks and slurry, the average equilibrium equivalent concentrations (EEC) of thoron and radon were 1.02 {+-} 0.48 and 16.41 {+-} 9.02 Bq m{sup -3}, respectively, whereas for city dwellings built of cement blocks and floor slabs, the results were 0.48 {+-} 0.47 and 11.50 {+-} 6.99 Bq m{sup -3} for thoron and radon, respectively. For outdoor air, concentrations of thoron and radon progeny were 0.29 {+-} 0.28 and 7.05 {+-} 2.68 Bq m{sup -3}, respectively. Radiation exposures from thoron and radon progeny were also evaluated; the ratio of dose contribution from thoron progeny to that of radon progeny was evaluated to be 28% and 17% in country houses and city dwellings, respectively. (note)

  2. Campylobacter epidemiology from breeders to their progeny in Eastern Spain.

    Science.gov (United States)

    Ingresa-Capaccioni, S; Jiménez-Trigos, E; Marco-Jiménez, F; Catalá, P; Vega, S; Marin, C

    2016-03-01

    While horizontal transmission is a route clearly linked to the spread of Campylobacter at the farm level, few studies support the transmission of Campylobacter spp. from breeder flocks to their offspring. Thus, the present study was carried out to investigate the possibility of vertical transmission. Breeders were monitored from the time of housing day-old chicks, then throughout the laying period (0 to 60 wk) and throughout their progeny (broiler fattening, 1 to 42 d) until slaughter. All samples were analyzed according with official method ISO 10272:2006. Results revealed that on breeder farms, Campylobacter isolation started from wk 16 and reached its peak at wk 26, with 57.0% and 93.2% of positive birds, respectively. After this point, the rate of positive birds decreased slightly to 86.0% at 60 wk. However, in broiler production all day-old chicks were found negative for Campylobacter spp, and the bacteria was first isolated at d 14 of age (5.0%), with a significant increase in detection during the fattening period with 62% of Campylobacter positive animals at the end of the production cycle. Moreover, non-positive sample was determined from environmental sources. These results could be explained because Campylobacter may be in a low concentration or in a non-culturable form, as there were several studies that successfully detected Campylobacter DNA, but failed to culture. This form can survive in the environment and infect successive flocks; consequently, further studies are needed to develop more modern, practical, cost-effective and suitable techniques for routine diagnosis. © 2016 Poultry Science Association Inc.

  3. Pre-selecting young bulls before progeny-testing with marker-assisted BLUP

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Suppose that a QTL affecting milk production trait of dairy cattle has been identified, and the relative distances between the QTL and the markers are known without error. The relative efficiency of pre-selection of young bulls before progeny-testing with marker-assisted BLUP (MBLUP) over conventional progeny-testing (i.e. random selection before progeny-testing) for five generations was studied using Monte Carlo simulation. Different numbers of QTL alleles (two or infinite), initial frequencies of the favorable QTL allele (0.1 and 0.5, when the QTL has two alleles), distances between the adjacent markers (5 cM or 20 cM), and ratios of the QTL variance to the genetic variance (10%, 25%, and 50%) were considered in the simulation. The results showed that the relative efficiency of MBLUP was higher when the marker distance was 5 cM than that when the distance was 20 cM. The larger the QTL allelic effect, the lower the MBLUP efficiency. The relative superiority of MBLUP was higher when the QTL had two alleles and the frequency of the favorable QTL allele was low in the base population. The relative superiority of MBLUP decreased with the progress of generation in all cases and the decreasing rate was getting higher with the increase of marker density and QTL effect.

  4. Multivariate analysis to determine the genetic distance among backcross papaya (Carica papaya) progenies.

    Science.gov (United States)

    Ramos, H C C; Pereira, M G; Gonçalves, L S A; Berilli, A P C G; Pinto, F O; Ribeiro, E H

    2012-05-14

    Morpho-agronomic and molecular (RAPD and ISSR markers) data were used to evaluate genetic distances between papaya backcross progenies in order to help identify agronomically superior genotypes. Thirty-two papaya progenies were evaluated based on 15 morpho-agronomic characteristics, 20 ISSR and 19 RAPD primers. Manhattan, Jaccard and Gower distances were used to estimate differences based on continuous and binary data and combined analyses, respectively. Except for production, there were significant differences in the continuous variables among the genotypes. The molecular analysis revealed 193 dominant markers (ISSR and RAPD), being 53 polymorphic loci. Among the various clusters that were generated, the one based on a combined analysis of morpho-agronomic and molecular data gave the highest cophenetic correlation (0.72) compared to individual analysis, consistently allocating the progenies into six groups. We found that the Gower algorithm was more coherent in the discrimination of the genotypes, demonstrating that a combination of molecular and agronomic data is valuable for studies of genetic dissimilarity in papaya.

  5. f~(-1)[f(x)]=x和f[f~(-1)(x)]=x(高一、高二、高三)

    Institute of Scientific and Technical Information of China (English)

    曾家骏

    2002-01-01

    请看下面两个题目: 1.已知函数f(x)=(3x-2)/(2x+7),则f[f-1(x)]=_______; 2.若g(x)=2x3+4,则g1[g(x)]=_______. 这是一本高中数学辅导书上的两个练习题,原解答是先分别求出反函数f-1(x)和g-1(x)后,再代入复合计算得出结果,答案都是x.其实,这些计算都是多余的,无论f(x)和

  6. Morphological and physiological responses of two coffee progenies to soil water availability.

    Science.gov (United States)

    Dias, Paulo C; Araujo, Wagner L; Moraes, Gustavo A B K; Barros, Raimundo S; DaMatta, Fábio M

    2007-12-01

    Drought is a major environmental constraint affecting growth and production of coffee. The effects of water supply on growth, biomass allocation, water relations, and gas exchange in two coffee progenies representing drought-tolerant (Siriema) and drought-sensitive (Catucaí) genotypes were compared. They were grown in 12-L pots until 4-months old, when they were submitted to two watering treatments for 60 d: plants receiving either 100% transpired water (control plants) or a fraction (about 40%) of the amount of water transpired by control plants (drought-stressed plants). Under control conditions, Siriema grew faster than Catucaí. Regardless of the watering regimes and progenies, relative growth rate (RGR) was positively correlated both with net assimilation rate (NAR) and long-term water-use efficiency (WUE), but not with differences in biomass allocation. Both progenies responded to drought stress through (i) similar decreases in both RGR and NAR with marginal, if any, changes in allocation; (ii) decreases in leaf water potential, which occurred to a greater extent in Catucaí than in Siriema, even though they have showed similar abilities to adjust osmotically and elastically; (iii) similar reductions in net photosynthesis due mainly to nonstomatal factors; and (iv) decreases in transpiration rate coupled with increased long-term WUE. However, the lower transpiration rate and the higher long-term WUE as found in Siriema relative to Catucaí under control conditions persisted under drought conditions. Overall, the major differences between these progenies were largely associated with differences in plant water use, which was likely related to the improved water status of Siriema. The possible implications of selecting coffee genotypes for high WUE are discussed.

  7. Piceatannol, a stilbene phytochemical, inhibits mitochondrial F0F1-ATPase activity by targeting the F1 complex.

    Science.gov (United States)

    Zheng, J; Ramirez, V D

    1999-08-02

    Piceatannol is a stilbene phytochemical from the seeds of Euphorbia lagascae, previously identified as an antileukemic principle. Piceatannol is considered an inhibitor of several tyrosine kinases. We recently reported that resveratrol, another stilbene phytoalexin from grape seeds, was an inhibitor of ATP synthase. Here, we demonstrated that piceatannol potently inhibited the rat brain mitochondrial F0F1-ATPase activity in both solubilized and submitochondrial preparations (IC50 of 8-9 microM), while having relatively small effect on the Na(+), K(+)-ATPase activity of porcine cerebral cortex (no effect up to 7 microM). Piceatannol inhibited the ATPase activity of the purified rat liver F1 with IC50 of about 4 microM, while resveratrol was slightly less active (IC50 of about 14 microM). Our results indicate that piceatannol and resveratrol inhibit the F-type ATPase by targeting the F1 sector, which is located to the inner membrane of mitochondria and plasma membrane of normal endothelial cells and several cancer cell lines. This mechanism could potentially contribute to the multiple effects of these chemopreventive phytochemicals. Copyright 1999 Academic Press.

  8. Observation of {sup 222}Rn progeny-and {sup 220}Rn progeny-loaded aerosols by atomic force microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Leung, J.K.C.; Tso, M.Y.W.; Lam, J.H.C. [The Univ., of Hong Kong, Hong Kong (China); Zhau, Q.F. [Ministry of Health, Beijing (China)

    2002-07-01

    Atomic force microscopy is becoming a powerful tool for the study of nuclear tracks in materials such as CR-39. Coupled with its capability of observing near nm aerosol particles, we have utilized the AFM to observe the radon progeny-loaded aerosol particles deposited on surfaces of CR-39 and to observe the corresponding etch pits produced by the {alpha} -particles emitted from the radon progenies. A special platform was built so that after the aerosol particles on the CR-39 have been scanned and recorded, the CR-39 can be etched and then scanned for the etch pits at the same location. Both {sup 222}Rn and {sup 220}Rn progenies were used in the study. The progenies were generated by the appropriate radon sources and mixed with aerosol particles generated by aerosol generators. The aerosol size distributions were analyzed by a scanning mobility particle sizer. Some of the limitations and difficulties of the technique will be described. The results enable us to examine the attachment process including multiple attachments of radon progenies on aerosols.

  9. Non-leptonic decays of $B \\to ( f_1(1285),f_1(1420) ) V$ in the perturbative QCD approach

    CERN Document Server

    Liu, Xin; Zou, Zhi-Tian

    2016-01-01

    We investigate the branching ratios, the polarization fractions, the direct CP-violating asymmetries, and the relative phases in 20 non-leptonic decay modes of $B \\to f_1 V$ within the framework of perturbative QCD approach at leading order with $f_1$ including two $^3\\!P_1$-axial-vector states $f_1(1285)$ and $f_1(1420)$. Here, $B$ denotes $B^+$, $B^0$, and $B_s^0$ mesons and $V$ stands for the lightest vector mesons $\\rho$, $K^*$, $\\omega$, and $\\phi$ , respectively. The $B_s^0 \\to f_1 V$ decays are studied theoretically for the first time in the literature. Together with the angle $\\phi_{f_1} \\approx (24^{+3.2}_{-2.7})^\\circ$ extracted from the measurement through $B_{d/s} \\to J/\\psi f_1(1285)$ modes for the $f_1(1285)-f_1(1420)$ mixing system, it is of great interest to find phenomenologically that some modes such as the tree-dominated $B^+ \\to f_1 \\rho^+$ and the penguin-dominated $B^{+,0} \\to f_1 K^{*+,0}, B_s^0 \\to f_1 \\phi$ with large branching ratios around ${\\cal O}(10^{-6})$ or even ${\\cal O}(10^{-...

  10. Effects of pedigree and exotic genetic inheritance on semen production traits of dairy bulls

    Institute of Scientific and Technical Information of China (English)

    VijethaBajjalliThippeswamy; SiddarthaShankar Layek; ArumugamKumaresan; TusharKumar Mohanty; Ashok Kumar Gupta; Atish Kumar Chakravarty; Ayyasamy Manimaran; Shiv Prasad

    2014-01-01

    Objective:To study the effects of different levels of exotic inheritance on ejaculate quality in bulls and its passage through different generations.Methods:Data on semen production traits and ejaculate quality were obtained for38 crossbred bulls and grandsire-sire-progeny relationship in relation to semen quality was studied.The bulls were classified into three groups based on the level of exotic inheritance viz.F1,50.0%-62.5% exotic germplasm and >75% exotic germplasm.Results:Results of the present study indicated that about40% of the ejaculates obtained from the crossbred bulls were rejected from further processing due to poor ejaculate quality.TheF1 bulls produced significantly higher proportions(57.00±10.00) of poor quality ejaculates compared to the interse mated bulls.The age at first semencollection in crossbred bulls ranged from567 to1010 days with an average of738.89±18.18 days while the mean age at first semen freezing was865.72±34.60 days.Conclusions:It may be inferred that the“acceptable quality semen producing ability” decreased from grandsire through sire to male progeny and among the increasing exotic genetic levels ofCB cattle,F1 bulls produced significantly higher“low grade ejaculates” that were unfit for cryopreservation.

  11. Behavior of {sup 220}Rn progeny in diffusion chamber

    Energy Technology Data Exchange (ETDEWEB)

    Nikezic, D. [Faculty of Science, University of Kragujevac, R. Domanovic 12, 34000 Kragujevac (Serbia)]. E-mail: nikezic@kg.ac.yu; Stevanovic, N. [Faculty of Science, University of Kragujevac, R. Domanovic 12, 34000 Kragujevac (Serbia)

    2007-01-01

    The distribution of {sup 220}Rn (thoron) within the cylindrical diffusion chamber decreases exponentially with the distance from the entry filter. The first {sup 220}Rn progeny {sup 216}Po follows its parent due to its very short half life. Other {sup 220}Rn progeny are almost completely deposited, but the deposition is not uniform. Deposition occurs on all internal surfaces of cylinder including vertical wall as well as its bases. Deposition on the upper and lower base is enhanced in the middle and drops toward to the end while the deposition on 'vertical' wall is larger closer to the entry membrane.

  12. Current international intercomparison measurement on radon and its progeny

    Energy Technology Data Exchange (ETDEWEB)

    Yamasaki, Keizo [Kyoto Univ., Kumatori, Osaka (Japan). Research Reactor Inst.

    1996-12-01

    The international intercomparison measurement on radon and its progeny was held between the EML of USDOE and several Japanese organisations, using the radon test chamber installed in EML. Japanese results of radon concentration by the active method using the ionization chamber or scintillation cell and the passive method using the solid track detector were about 5% small compared to that of EML. On the results of radon progeny, there were not any large systematic differences between EML and Japanese participants in spite of wide range of deviation except for the results at the condition of low aerosol density. (author)

  13. Comparisons of Photosynthetic Characteristics in Relation to Lint Yield Among F1 Hybrids, Their F2 Descendants and Parental Lines of Cotton

    Institute of Scientific and Technical Information of China (English)

    FENG Guo-yi; GAN Xiu-xia; YAO Yan-di; LUO Hong-hai; ZHANG Ya-li; ZHANG Wang-feng

    2014-01-01

    We compared the photosynthetic characteristics in relation to yield of two F1 cotton hybrids (Shiza 2-F1 and Xinluzao 43-F1), their parental lines (NT2, H2 and 4-14) and their F2 descendants at different growth and development stages. The two F1 exhibited heterobeltiosis in net photosynthetic rate (Pn) by 8.1-52.1%, canopy apparent photosynthetic rate (CAP) by 8.2-57.6% and canopy respiration rate (CR) by 3.0-78.7% during the growing season. They also exhibited mid-parent heterosis by 2.0-5.2% in leaf chlorophyll content (SPAD) during the late growth and development stages. Regression analysis showed that both parents contributed to increase in Pn, SPAD and CAP in the F1. A low CR in the F1 matched a low CR of the parental line. Photosynthetic characteristics in the F2 were mainly dependent upon the magnitude and degeneration rate of the F1. Mid-parent heterosis in CAP and in CR during the late growth and development stage reduced the degeneration of the F2. Average dry matter accumulation was 10.7-34.7% higher in the parental lines of Xinluzao 43-F1 than in the parental lines of Shiza 2-F1. Heterobeltiosis in dry matter accumulation was 7.0-23.1% greater for Xinluzao 43-F1 than for Shiza 2-F1. Dry matter accumulation in the F1 was affected by either the dry matter accumulation of parents or heterobeltiosis. Dry matter accumulation in the F2 was mainly inlfuenced by dry matter accumulation in the F1. The yields of the two F1 were 39.1-46.3% higher than their respective parents and 26.4-45.9% higher than that of the conventional cultivar Xinluzao 33. The yields of the two F2 were 9.2-12.8% higher than the parents and 14.9-27.4% higher than that of Xinluzao 33. The photosynthetic production and yield of the F1 and F2were higher than that of their parents. The increases in Pn and CAP of the F1 and F2 were dependent on the photosynthetic characteristics of their parents. It is thus concluded that the photosynthetic performance, light use efifciency and yield of the F1

  14. Cleanup Verification Package for the 118-F-1 Burial Ground

    Energy Technology Data Exchange (ETDEWEB)

    E. J. Farris and H. M. Sulloway

    2008-01-10

    This cleanup verification package documents completion of remedial action for the 118-F-1 Burial Ground on the Hanford Site. This burial ground is a combination of two locations formerly called Minor Construction Burial Ground No. 2 and Solid Waste Burial Ground No. 2. This waste site received radioactive equipment and other miscellaneous waste from 105-F Reactor operations, including dummy elements and irradiated process tubing; gun barrel tips, steel sleeves, and metal chips removed from the reactor; filter boxes containing reactor graphite chips; and miscellaneous construction solid waste.

  15. On fundamental groups related to the Hirzebruch surface F1

    Institute of Scientific and Technical Information of China (English)

    Michael; FRIEDMAN; Mina; TEICHER

    2008-01-01

    Given a projective surface and a generic projection to the plane,the braid monodromy factorization(and thus,the braid monodromy type)of the complement of its branch curve is one of the most important topological invariants,stable on deformations.From this factorization,one can compute the fundamental group of the complement of the branch curve,either in C2 or in CP2.In this article,we show that these groups,for the Hirzebruch surface F1,(a,b),are almost-solvable.That is, they are an extension of a solvable group,which strengthen the conjecture on degeneratable surfaces.

  16. On fundamental groups related to the Hirzebruch surface F1

    Institute of Scientific and Technical Information of China (English)

    Michael FRIEDMAN; Mina TEICHER

    2008-01-01

    Given a.projective surface and a generic projection to the plane,the braid monodromy factorization (and thus,the braid monodromy type) of the complement of its branch curve is one of the most important topological invariants,stable on deformations.From this factorization,one can compute the fundamental group of the complement of the branch curve,either in C2 or in CP2.In this article,we show that these groups,for the Hirzebruch surface F1,(a,b),are almost-solvable.That is,they are an extension of a solvable group,which strengthen the conjecture on degeneratable surfaces.

  17. E2F-1 as an anticancer drug target

    Directory of Open Access Journals (Sweden)

    Joseph R. Bertino

    2011-12-01

    Full Text Available Mounting evidence indicates that the E2F transcription factors play an essential role in all aspects of cellular functions. Many human malignancies have been shown to overexpress one or more of the ‘‘activating’’ E2Fs. In some circumstances, down regulation as well as overexpression of E2F-1, leads to inhibition of cell growth. The emphasis in this review is placed on new data implicating microRNAs in the regulation of E2F activity and the efforts thus far to target this activity in order to cause tumor regression.

  18. Data of evolutionary structure change: 1COBB-1F1GD [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1COBB-1F1GD 1COB 1F1G B D ATKAVCVLKGDGPVQGTIHFEAKG--DTVVVTGSITGLTE-GDHGFHVHQFGD...NTQGCTSAGPHFNPLSKKHGGPKDEERHVGDLGNVTADKNGVAIVDIVDPLISLSGEYSIIGRTMVVHEKPDDLGRGGNEESTKTGNAGSRLAC...GVIGIAK -VQAVAVLKGDAGVSGVVKFEQASESEPTTVSYEIAGNSPNAERGFHIHEFGDATNGCVSAGPHFNPFKKTHGAPTDEVRHVGDMGN...VKTDENGVAKGSFKDSLIKLIGPTSVVGRSVVIHAGQDDLGKGDTEESLKTGNAGPRPACGVIGLTN ...pdbID>1F1G D 1F1GD FEQASESEPTTV

  19. cDNA library Table: F1mg [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available F1mg NA F1mg F1 (J150 x J203) midgut fourth instar larval stage D2 mixed pBluescrip...t SK- EcoR1 for 5' Xho1for 3' sequenced from T3 primer (5' -> 3') BY917461-BY918785,BY925786-BY927071 E_ET_F1mg_[number]_F_0,E_ET_F1mg_[number]_R_0 ...

  20. Studies on Morphology of F1 Hybrid and Systematic Evolution of Epimedium Species%淫羊藿属植物杂交F1代植株形态和种间系统演化研究

    Institute of Scientific and Technical Information of China (English)

    杜明凤; 陈庆富

    2013-01-01

    淫羊藿属植物多数种类在形态分类性状上存在连续过渡的形态变异现象,导致种间的鉴别困难.本研究对粗毛淫羊藿、巫山淫羊藿、天平淫羊藿,3个物种合计29个居群之间的种间杂交F1代植株及其亲本形态学进行了比较研究.结果表明:AC×MY、AC×WU、WU×MY 3种组合杂交F1代的叶片形状表现为双亲过渡形态,控制淫羊藿花瓣距短于内萼片的基因可能是部分显性,E.acuminatum的花瓣和内萼片颜色属于显性遗传.所得结果为淫羊藿属植物分类学、种间系统关系及其起源与进化研究提供了新的资料和线索.%It is known that there are continuous variations of morphological characteristics for taxonomy among most Epimedium species,leading to difficulties of species identification.The morphology of interspecific F1 hybrid plants among 29 populations of E.acuminatum,E.wushanese and E.myrianthum was studied comparatively.The results shows that the length of flower calcar shorter than the inner sepal may be controlled by partly-dominant gene,and the leaf shape of hybrid F1 progenies are the middle type between the parents.The colour of the inner sepal and flower calcar in E.acuminatum is controlled by dominant genes.These above results are useful on interspecific hybridization and variety improvement on Epimedium.

  1. E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity.

    Science.gov (United States)

    Sahin, Fikret; Sladek, Todd L

    2010-07-04

    E2F-1 is the major cellular target of pRB and is regulated by pRB during cell proliferation. Interaction between pRB and E2F-1 is dependent on the phosphorylation status of pRB. Despite the fact that E2F-1 and pRB have antagonistic activities when they are overexpressed, the role of the E2F-1-pRB interaction in cell growth largely remains unknown. Ideally, it would be better to study the properties of a pRB mutant that fails to bind to E2F, but retains all other activities. To date, no pRB mutation has been characterized in sufficient detail to show that it specifically eliminates E2F binding but leaves other interactions intact. An alternative approach to this issue is to ask whether mutations that change E2F proteins binding affinity to pRB are sufficient to change cell growth in aspect of cell cycle and tumor formation. Therefore, we used the E2F-1 mutants including E2F-1/S332-7A, E2F-1/S375A, E2F-1/S403A, E2F-1/Y411A and E2F-1/L132Q that have different binding affinities for pRB to better understand the roles of the E2F-1 phosphorylation and E2F-1-pRB interaction in the cell cycle, as well as in transformation and gene expression. Data presented in this study suggests that in vivo phosphorylation at amino acids 332-337, 375 and 403 is important for the E2F-1 and pRB interaction in vivo. However, although E2F-1 mutants 332-7, 375 and 403 showed similar binding affinity to pRB, they showed different characteristics in transformation efficiency, G(0) accumulation, and target gene experiments.

  2. Thoron and thoron progeny measurements in German clay houses.

    Science.gov (United States)

    Gierl, S; Meisenberg, O; Feistenauer, P; Tschiersch, J

    2014-07-01

    In recent years, elevated thoron concentrations were found in houses built of unfired clay. In this study experiments were carried out in 17 traditional and modern clay houses in Germany to obtain an overview of indoor thoron in such houses. Long-term measurements over an 8-week period were performed using a newly developed Unattended Battery-Operated Progeny Measurement Device (UBPM) for measuring thoron progeny. This instrument uses a high-voltage electric field to precipitate radon and thoron progeny on nuclear track detectors. Additional active and passive measurements of radon, thoron and their progeny were performed. The equilibrium equivalent thoron concentration was found to be between 2 and 10 Bq m(-3). Gas concentrations were found to be between 20 and 160 Bq m(-3) for radon and between 10 and 90 Bq m(-3) for thoron 20 cm from the wall. The thoron exposure contributes significantly to the inhalation dose of the dwellers (0.6-4 mSv a(-1)).

  3. Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis.

    Science.gov (United States)

    Czernicka, Małgorzata; Pławiak, Jarosław; Muras, Piotr

    2014-01-01

    Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent--B. nana and 12 hybrids and cluster II grouped the male parent--B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.

  4. Variations in wood anatomical properties and specific gravity of half sib progenies of Populus deltoides

    Institute of Scientific and Technical Information of China (English)

    P. K. Pande; R. C. Dhiman

    2012-01-01

    We studied radial and inter-progeny variations in the dimensions of the wood elements and specific gravity of 21 half sib progenies of Populus deltoides Bartr.ex Marsh.The female parents of half sib progenies were G48 and S7C13 clones.Variance ratio (F) test indicated that inter-progeny variations in the dimensions of wood elements and specific gravity were significant for all wood traits while variations were significant for radial location for specific gravity only.Hierarchical cluster analysis was done by Squared Euclidean Distance for all of 21 progenies considering six wood traits.21 progenies were grouped into 4 clusters.Cluster 1 was the largest cluster with 11 progenies,whereas cluster 3 had only one progeny.Selected progenies in dusters 3 (progeny 155,male) and 4 (progeny 108,196,both female) were highly divergent from the other progenies so they were used in combinations as parents of hybrids to develop new clones with desired characters.Progeny 155 showed higher growth,fiber dimensions,and specific gravity,thus,should be used for the development of new clones.

  5. Defining the nature of human pluripotent stem cell progeny

    Institute of Scientific and Technical Information of China (English)

    Michaela Patterson; David N Chan; Iris Ha; Dana Case; Yongyan Cui; Ben Van Handel; Hanna KA Mikkola; William E Lowry

    2012-01-01

    While it is clear that human pluripotent stem cells (hPSCs) can differentiate to generate a panoply of various cell types,it is unknown how closely in vitro development mirrors that which occurs in vivo.To determine whether human embryonic stem cells (hESCs) and human-induced pluripotent stem cells (hiPSCs) make equivalent progeny,and whether either makes cells that are analogous to tissue-derived cells,we performed comprehensive transcriptome profiling of purified PSC derivatives and their tissue-derived counterparts.Expression profiling demonstrated that hESCs and hiPSCs make nearly identical progeny for the neural,hepatic,and mesenchymal lineages,and an absence of re-expression from exogenous reprogramming factors in hiPSC progeny.However,when compared to a tissuederived counterpart,the progeny of both hESCs and hiPSCs maintained expression of a subset of genes normally associated with early mammalian development,regardless of the type of cell generated.While pluripotent genes (OCT4,SOX2,REX1,and NANOG) appeared to be silenced immediately upon differentiation from hPSCs,genes normally unique to early embryos (LIN28A,LIN28B,DPPA4,and others) were not fully silenced in hPSC derivatives.These data and evidence from expression patterns in early human fetal tissue (3-16 weeks of development) suggest that the differentiated progeny of hPSCs are reflective of very early human development (< 6 weeks).These findings provide support for the idea that hPSCs can serve as useful in vitro models of early human development,but also raise important issues for disease modeling and the clinical application of hPSC derivatives.

  6. Maternal diet supplemented with methyl-donors protects against atherosclerosis in F1 ApoE(-/- mice.

    Directory of Open Access Journals (Sweden)

    Colin Delaney

    Full Text Available Atherosclerosis is an inflammatory condition of the arterial wall mediated by cells of both innate and adaptive immunity. T lymphocytes play an important role in orchestrating the pathogenic immune response involved in the acceleration of atherosclerosis. Previously, we have shown that a prenatal methyl-donor supplementation diet (MS, when fed to dams during pregnancy and lactation, decreased the T cell-mediated pro-inflammatory cytokine and chemokine response in F1 mice. In the current study, we report feeding Apolipoprotein E (ApoE(-/- deficient dams with the MS diet during pregnancy reduces atherosclerotic plaques in F1 mice that were fed high fat diet (HFD after weaning. F1 mice from dams on the MS diet exhibited increased global T cell DNA methylation. T-cell chemokines and their receptors (in particular CCR2, CCR5, and CXCR3 play important roles in the inflammatory cell recruitment to vascular lesions. MS diet significantly reduced Ccr2 mRNA and protein expression in CD3+ T cells but not in CD11b+ monocytes in MS F1 mice relative to controls. F1 litter size, HFD consumption, body weight, and body fat were similar between control and MS diet groups. Moreover, serum thiol metabolite levels were similar between the two groups. However, MS diet is associated with significantly higher serum HDL and lower LDL+VLDL levels in comparison to F1 mice from dams on the control diet. Inflammatory cytokines (IL-17, TNF-α, IL-6 were also lower in MS F1 mice serum and conditioned media from T-cell culture. Altogether, these data suggest that the MS diet ameliorates development of atherosclerosis by inhibiting the T-cell Ccr2 expression, reducing inflammatory cytokines production and increasing serum HDL:LDL ratio.

  7. Segregation ratios of colored grains in F1 hybrid wheat

    Directory of Open Access Journals (Sweden)

    Zifeng Guo

    2012-01-01

    Full Text Available Nutritious and functional foods from wheat have received great attention in recent years. Colored-grain wheat contains a large number of nutrients such as anthocyanins and hence the breeding is interesting. In this work, colored-grained wheat lines of mixed pollination of einkorn wheat (Triticum boeoticum, AA and French rye (French Secale cereale, RR were used as male parents and wheat line Y1642 (derived from common wheat and Agropyron elongatum, AABBDD was used as the female parent. These colored wheat were used for diallel cross to study the segregation ratios of F1 colored grains. Results show that the color inheritance of purple-grained wheat follows a maternal inheritance pattern and that the blue-grained wheat expresses xenia in most cases. In some circumstances, the grains with different color shades appear in the same spike.

  8. Single molecule energetics of F1-ATPase motor.

    Science.gov (United States)

    Muneyuki, Eiro; Watanabe-Nakayama, Takahiro; Suzuki, Tetsuya; Yoshida, Masasuke; Nishizaka, Takayuki; Noji, Hiroyuki

    2007-03-01

    Motor proteins are essential in life processes because they convert the free energy of ATP hydrolysis to mechanical work. However, the fundamental question on how they work when different amounts of free energy are released after ATP hydrolysis remains unanswered. To answer this question, it is essential to clarify how the stepping motion of a motor protein reflects the concentrations of ATP, ADP, and P(i) in its individual actions at a single molecule level. The F(1) portion of ATP synthase, also called F(1)-ATPase, is a rotary molecular motor in which the central gamma-subunit rotates against the alpha(3)beta(3) cylinder. The motor exhibits clear step motion at low ATP concentrations. The rotary action of this motor is processive and generates a high torque. These features are ideal for exploring the relationship between free energy input and mechanical work output, but there is a serious problem in that this motor is severely inhibited by ADP. In this study, we overcame this problem of ADP inhibition by introducing several mutations while retaining high enzymatic activity. Using a probe of attached beads, stepping rotation against viscous load was examined at a wide range of free energy values by changing the ADP concentration. The results showed that the apparent work of each individual step motion was not affected by the free energy of ATP hydrolysis, but the frequency of each individual step motion depended on the free energy. This is the first study that examined the stepping motion of a molecular motor at a single molecule level with simultaneous systematic control of DeltaG(ATP). The results imply that microscopically defined work at a single molecule level cannot be directly compared with macroscopically defined free energy input.

  9. The F1-F2 vowel chart for Czech whispered vowels a, e, i, o, u.

    Science.gov (United States)

    Grepl, Michal; Furst, Tomas; Pesak, Josef

    2007-12-01

    The aim of this contribution is to present the formant chart of the Czech vowels a, e, i, o, u and show that this can be achieved by means of digital methods of sound processing. A group of 35 Czech students of the Pedagogical Faculty of Palacky University was tested and a record of whispered vowels was taken from each of them. The record was digitalized and processed by the Discrete Fourier Trasform. The result is the power spectrum of the individual vocals - the graphic output consists of a plot of the relative power of individual frequencies in the original sound. The values of the first two maxima which represent the first and the second formants were determined from the graph. The values were plotted on a formant chart. Altogether, 175 spectral analyses of individual vowels were performed. In the resulting power spectrum, the first and the second formant frequencies were identified. The first formant was plotted against the second one and pure vocal formant regions were identified. Frequency bands for the Czech vowel "a" were circumscribed between 850 and 1150 Hz for first formant (F1) and between 1200 and 2000 Hz for second formant (F2). Similarly, borders of frequency band for vowel "e" they were 700 and 950 Hz for F1 and 1700 and 3000 Hz for F2. For vowel "i" 300 and 450 Hz for F1 and 2000 and 3600 Hz for F2, for vowel "o" 600 and 800 Hz for F1 and 600 and 1400 Hz for F2, for vowel "u" 100 and 400 Hz for F1 and 400 and 1200 Hz for F2. At low frequencies it is feasible to invoke the source-filter model of voice production and associate vowel identity with frequencies of the first two formants in the voice spectrum. On the other hand, subject to intonation, singing or other forms of exposed voice (such as emotional speech, focused speech), the formant regions tend to spread. In spectral analysis other frequencies dominate, so specific formant frequency bands are not easily recognizable. Although the resulting formant map is not much different from the formant

  10. Comparative analysis on natural spawning of F1 meagre, Argyrosomus regius, with wild broodstock spawns in Portugal.

    Science.gov (United States)

    Soares, Florbela; Ribeiro, Laura; Gamboa, Margarida; Duarte, Sílvia; Mendes, Ana Candeias; Castanho, Sara; Barata, Marisa; Lourenço, Tânia Margarida; Pousão-Ferreira, Pedro

    2015-12-01

    The purpose of this study was to describe and compare the reproductive success and egg and larvae quality between wild and first-generation (F1) breeders of Argyrosomus regius. Wild broodstock were adapted to captivity, and good-quality spawns were obtained in 2009-2010, after GnRH treatment. In 2012, the F1 meagre (3 years old) spawned naturally at IPMA's Aquaculture Research Station facilities. From each spawning event, the following parameters were determined: number of floating and non-floating eggs, egg size, hatching success and larval total length. Eggs size and percentage of hatching obtained from F1 breeders (1.04 ± 0.10 mm and 90.5 ± 6.4%) were significantly higher when compared with wild breeders (0.97 ± 0.13 mm and 17.0 ± 12.7%). Although wild breeder spawns exhibited 2.7 ± 0.2 mm for larval total length, F1 breeder spawns presented 2.6 ± 0.2 mm. The wild and F1 breeder spawns exhibit a good egg and larval quality, indicating a promising starting point for a successful meagre hatchery production.

  11. The F(0F(1-ATP synthase complex contains novel subunits and is essential for procyclic Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Alena Zíková

    2009-05-01

    Full Text Available The mitochondrial F(0F(1 ATP synthase is an essential multi-subunit protein complex in the vast majority of eukaryotes but little is known about its composition and role in Trypanosoma brucei, an early diverged eukaryotic pathogen. We purified the F(0F(1 ATP synthase by a combination of affinity purification, immunoprecipitation and blue-native gel electrophoresis and characterized its composition and function. We identified 22 proteins of which five are related to F(1 subunits, three to F(0 subunits, and 14 which have no obvious homology to proteins outside the kinetoplastids. RNAi silencing of expression of the F(1 alpha subunit or either of the two novel proteins showed that they are each essential for the viability of procyclic (insect stage cells and are important for the structural integrity of the F(0F(1-ATP synthase complex. We also observed a dramatic decrease in ATP production by oxidative phosphorylation after silencing expression of each of these proteins while substrate phosphorylation was not severely affected. Our procyclic T. brucei cells were sensitive to the ATP synthase inhibitor oligomycin even in the presence of glucose contrary to earlier reports. Hence, the two novel proteins appear essential for the structural organization of the functional complex and regulation of mitochondrial energy generation in these organisms is more complicated than previously thought.

  12. An ester extract of Cochinchina momordica seeds induces differentiation of melanoma B16 F1 cells via MAPKs signaling.

    Science.gov (United States)

    Zhao, Lian-Mei; Han, Li-Na; Ren, Feng-Zhi; Chen, Shu-Hong; Liu, Li-Hua; Wang, Ming-Xia; Sang, Mei-Xiang; Shan, Bao-En

    2012-01-01

    Cochinchina momordica seeds (CMS) have been widely used due to antitumor activity by Mongolian tribes of China. However, the details of the underlying mechanisms remain unknown. In the present study, we found that an EtOAc (ethyl ester) extract of CMS (CMSEE) induced differentiation and caused growth inhibition of melanoma B16 F1 cells. CMSEE at the concentration of 5-200 μg/ml exhibited strongest anti-proliferative effects on B16 F1 cells among other CMS fractions (water or petroleum ether). Moreover, CMSEE induced melanoma B16 F1 cell differentiation, characterized by dendrite-like outgrowth, increasing melanogenesis production, as well as enhancing tyrosinase activity. Western blot analysis showed that sustained phosphorylation of p38 MAP accompanied by decrease in ERK1/2 and JNK dephosphorylation were involved in CMSEE-induced B16 F1 cell differentiation. Notably, 6 compounds that were isolated and identified may be responsible for inducing differentiation of CMSEE. These results indicated that CMSEE contributes to the differentiation of B16 F1 cells through modulating MAPKs activity, which may throw some light on the development of potentially therapeutic strategies for melanoma treatment.

  13. Complete kinetic and thermodynamic characterization of the unisite catalytic pathway of Escherichia coli F1-ATPase. Comparison with mitochondrial F1-ATPase and application to the study of mutant enzymes.

    Science.gov (United States)

    Al-Shawi, M K; Senior, A E

    1988-12-25

    A complete analysis is presented of the component rate constants of the "unisite" reaction pathway in normal Escherichia coli F1-ATPase. Gibbs free energy profiles of the unisite reaction pathway were constructed for both normal E. coli F1 and bovine-heart mitochondrial F1, and comparison indicated that E. coli F1 is an ancestral form of the mitochondrial enzyme. Similar kinetic and thermodynamic analyses of the unisite reaction pathway were done for mutant beta-Asn-242 and beta-Val-242 E. coli F1-ATPases. Both mutations affected unisite binding and hydrolysis of MgATP but had little effect on release of products or binding of MgADP. It was apparent that a primary effect of the mutations was on the interaction between the catalytic nucleotide-binding domain and the substrate MgATP. The catalytic transition state [F1-ATP]++ was the most destabilized step in the reaction sequence. Measurements of delta delta G[F1.ATP]++ and linear free energy plots for the catalytic step were consistent with the view that, in normal enzyme, residue beta-Asp-242 accepts an H-bond from the transition-state substrate in order to facilitate catalysis. Both mutations impaired positive catalytic cooperativity. This was caused by energetic destabilization of the catalytic transition state and was an indirect effect, not a direct effect on signal transmission per se between catalytic nucleotide-binding domains on beta-subunits. Therefore, impairment of unisite catalysis and of positive catalytic cooperativity appeared to be linked. This may provide a unifying explanation as to why a series of other, widely separated mis-sense mutations within the catalytic nucleotide-binding domain on F1-beta-subunit, which have been reported to affect unisite catalysis, also impair positive catalytic cooperativity. Linear free energy plots for the ATP-binding step of unisite catalysis demonstrated that beta-Asn-242 and beta-Val-242 mutant enzymes did not suffer any gross disruptive change in structure of

  14. Effect of indoor-generated airborne particles on radon progeny dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Trassierra, C. Vargas [Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Cassino, FR (Italy); Stabile, L., E-mail: l.stabile@unicas.it [Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Cassino, FR (Italy); Cardellini, F.; Morawska, L. [National Institute of Ionizing Radiation Metrology (INMRI-ENEA), Rome (Italy); Buonanno, G. [Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Cassino, FR (Italy); International Laboratory for Air Quality and Health, Queensland University of Technology, Brisbane (Australia)

    2016-08-15

    Highlights: • Investigation of the interaction between particles and radon progeny dynamics. • Measurements of particles emitted by different indoor sources. • Tests performed in a controlled radon chamber. • Particle size strongly influences the radon progeny dynamics. • Particle surface area concentration is the key parameter of the radon-particle interaction. - Abstract: In order to investigate the interaction between radon progeny and particles, an experimental campaign was carried out in a radon chamber at the Italian National Institute of Ionizing Radiation Metrology, quantifying the amount of attached and unattached radon daughters present in air, as well as the equilibrium factor in the presence of particles generated through indoor sources. A fixed radon concentration was maintained, while particles were generated using incense sticks, mosquito coils and gas combustion. Aerosols were characterized in terms of particle concentrations and size distributions. Simultaneously, radon concentration and attached/unattached potential alpha energy concentration in the air were continuously monitored by two different devices, based on alpha spectroscopy techniques. The presence of particles was found to affect the attached fraction of radon decay products, in such a way that the particles acted as a sink for radionuclides. In terms of sources which emit large particles (e.g. incense, mosquito coils), which greatly increase particle surface area concentrations, the Equilibrium Factor was found to double with respect to the background level before particle generation sessions. On the contrary, the radon decay product dynamics were not influenced by gas combustion processes, mainly due to the small surface area of the particles emitted.

  15. Construction and comparative analyses of highly dense linkage maps of two sweet cherry intra-specific progenies of commercial cultivars.

    Directory of Open Access Journals (Sweden)

    Carolina Klagges

    Full Text Available Despite the agronomical importance and high synteny with other Prunus species, breeding improvements for cherry have been slow compared to other temperate fruits, such as apple or peach. However, the recent release of the peach genome v1.0 by the International Peach Genome Initiative and the sequencing of cherry accessions to identify Single Nucleotide Polymorphisms (SNPs provide an excellent basis for the advancement of cherry genetic and genomic studies. The availability of dense genetic linkage maps in phenotyped segregating progenies would be a valuable tool for breeders and geneticists. Using two sweet cherry (Prunus avium L. intra-specific progenies derived from crosses between 'Black Tartarian' × 'Kordia' (BT×K and 'Regina' × 'Lapins'(R×L, high-density genetic maps of the four parental lines and the two segregating populations were constructed. For BT×K and R×L, 89 and 121 F(1 plants were used for linkage mapping, respectively. A total of 5,696 SNP markers were tested in each progeny. As a result of these analyses, 723 and 687 markers were mapped into eight linkage groups (LGs in BT×K and R×L, respectively. The resulting maps spanned 752.9 and 639.9 cM with an average distance of 1.1 and 0.9 cM between adjacent markers in BT×K and R×L, respectively. The maps displayed high synteny and co-linearity between each other, with the Prunus bin map, and with the peach genome v1.0 for all eight LGs (LG1-LG8. These maps provide a useful tool for investigating traits of interest in sweet cherry and represent a qualitative advance in the understanding of the cherry genome and its synteny with other members of the Rosaceae family.

  16. Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars

    Science.gov (United States)

    Quero-García, José; Guzmán, Alejandra; Mansur, Levi; Gratacós, Eduardo; Silva, Herman; Rosyara, Umesh R.; Iezzoni, Amy; Meisel, Lee A.; Dirlewanger, Elisabeth

    2013-01-01

    Despite the agronomical importance and high synteny with other Prunus species, breeding improvements for cherry have been slow compared to other temperate fruits, such as apple or peach. However, the recent release of the peach genome v1.0 by the International Peach Genome Initiative and the sequencing of cherry accessions to identify Single Nucleotide Polymorphisms (SNPs) provide an excellent basis for the advancement of cherry genetic and genomic studies. The availability of dense genetic linkage maps in phenotyped segregating progenies would be a valuable tool for breeders and geneticists. Using two sweet cherry (Prunus avium L.) intra-specific progenies derived from crosses between ‘Black Tartarian’ × ‘Kordia’ (BT×K) and ‘Regina’ × ‘Lapins’(R×L), high-density genetic maps of the four parental lines and the two segregating populations were constructed. For BT×K and R×L, 89 and 121 F1 plants were used for linkage mapping, respectively. A total of 5,696 SNP markers were tested in each progeny. As a result of these analyses, 723 and 687 markers were mapped into eight linkage groups (LGs) in BT×K and R×L, respectively. The resulting maps spanned 752.9 and 639.9 cM with an average distance of 1.1 and 0.9 cM between adjacent markers in BT×K and R×L, respectively. The maps displayed high synteny and co-linearity between each other, with the Prunus bin map, and with the peach genome v1.0 for all eight LGs (LG1–LG8). These maps provide a useful tool for investigating traits of interest in sweet cherry and represent a qualitative advance in the understanding of the cherry genome and its synteny with other members of the Rosaceae family. PMID:23382953

  17. F1t3 RECEPTOR EXPRESSION ON THE SURFACE OF MALIGNANT HEMATOPOIETIC CELLS AND RESPONSES TO F1t3 LIGAND STIMULATION

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective: To investigate the F1t3 receptor expression on the surface of malignant hematopoietic cells, the effect of TNFa and dexamethasone (DXM) on its expression and the responses of those cells to recombinant human F1t3 ligand (rhFL). Methods: Eighteen malignant hematopoietic cell lines were determined for the F1t3 receptor expression by flow cytometric analysis. The effect of rhFL on the proliferation of malignant hematopoietic cells in vitro was measured using MTT assay. Results: The expressions of F1t3 receptor on the surface of Raji, Daudi, HL-60, 8266 and XG-6 cells were detected by flow cytometric analysis. Following incubation with 20 ng/ml TNFa for 24h, the number of F1t3 receptor positive cells decreased in Raji and 8266, increased in HL-60 and XG-6, and no difference in Daudi cells. After incubation with 10-6 mol/L DXM for 24h, the number of F1t3 receptor positive cells decreased in all the 5 F1t3 receptor positive cell lines. rhFL stimulated the proliferation of HL-60 and Raji cells. Conclusion: For most of the malignant hematopoietic cells, there was neither the expression of F1t3 receptor nor the response to rhFL. DXM may be useful to reduce the effect of FL on the proliferation of some F1t3 receptor positive malignant hematopoietic cells in vitro and in vivo.

  18. Selection of Arabic coffee progenies with rust resistance

    Directory of Open Access Journals (Sweden)

    Gustavo Hiroshi Sera

    2014-07-01

    Full Text Available The aim of this study was to select high-yielding coffee progenies with resistance to coffee leaf rust for the State of Paraná (Brazil. Field experiments in a randomized block design were carried out in Itaguajé and Congonhinhas. Yield, vegetative vigor and rust resistance of nine progenies of Arabic coffee and three check cultivars were evaluated. Many genotypes derived from “Sarchimor” and “Catucaí” were susceptible. Three coffee genotypes of Sarchimor germplasm and the F6 generation of genotype “Catuaí x (Catuaí x BA-10 coffee” were selected to advance generations and are promising to become new cultivars for being higher-yielding than ‘IAPAR 59’ and ‘Tupi IAC 1669-33’ and having a large number of plants with complete rust resistance.

  19. Prediction of genetic gains from selection in Arabica coffee progenies.

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Baião de Oliveira

    2011-01-01

    Full Text Available Gains from selection for yield were estimated in Arabica coffee progenies carrying rust-resistance genes. Theexperiment in augmented block design was installed in Três Pontas, state of Minas Gerais. Three blocks were established with sixplants per plot, spaced 3.50 x 0.90 m, in 96 regular (F2 progenies and two control treatments. The plant response to rust wasevaluated on a grade scale in 2008. Yield (bags per hectare was estimated in the growing seasons 2005 to 2008. Significantdifferences between treatments for yield were observed in all harvests, except 2005. The presence of genetic variability amongprogenies allowed significant gain from selection for yield. Under the experimental conditions of this study, selection for yield can beperformed in the first high-yield year, without major losses compared to genetic gain from selection for yield when based on the meanof four harvests.

  20. Mg2+ coordination in catalytic sites of F1-ATPase.

    Science.gov (United States)

    Weber, J; Hammond, S T; Wilke-Mounts, S; Senior, A E

    1998-01-13

    Coordination of the Mg2+ ion in Mg-nucleotide substrates by amino acid residue side chains in the catalytic site of Escherichia coli F1-ATPase was investigated. From the X-ray structure of the mitochondrial enzyme [Abrahams, J. P., Leslie, A. G. W., Lutter, R., and Walker, J. E. (1994) Nature 370, 621-628], it may be inferred that the hydroxyl of betaThr-156 is a direct ligand of Mg2+, whereas the carboxyls of betaGlu-181, betaGlu-185, and betaAsp-242 might contribute via intervening water molecules. Elimination of each respective functional group by site-directed mutagenesis, followed by determination of Mg-nucleotide and uncomplexed nucleotide binding affinities using a tryptophan probe, showed that betaThr-156, betaGlu-185, and betaAsp-242 are all involved in Mg2+ coordination, whereas betaGlu-181 is not. A derived structural model for the octahedral coordination around the Mg2+ ion is presented. The results indicate that the ADP-containing site in the X-ray structure is the catalytic site of highest affinity. Correct Mg2+ coordination is required for catalytic activity at physiological rates. Elimination of any one of the Mg2+-coordinating residues led to complete loss of Mg2+-dependent nucleotide binding cooperativity of the catalytic sites.

  1. The biomechanical properties of F1C pili

    CERN Document Server

    Castelain, Mickaël; Klinth, Jeanna; Lindberg, Stina; Andersson, Magnus; Uhlin, Bernt Eric; Axner, Ove

    2014-01-01

    Uropathogenic Escherichia coli (UPEC) express various kinds of organelles, so-called pili or fimbriae, that mediate adhesion to host tissue in the urinary tract through specific receptor-adhesin interactions. The biomechanical properties of these pili have been considered important for the ability of bacteria to withstand shear forces from rinsing urine flows. Force measuring optical tweezers have been used to characterize individual organelles of F1C type expressed by UPEC bacteria with respect to such properties. Qualitatively, the force-vs.-elongation response was found to be similar to that of other types of helix-like pili expressed by UPEC, i.e. type 1, P, and S, with force-induced elongation in three regions of which one represents the important uncoiling mechanism of the helix-like quaternary structure. Quantitatively, the steady-state uncoiling force was assessed to 26.4(1.4) pN, which is similar to those of other pili (which range from 21 pN for SI to 30 pN for type 1). The corner velocity for dynam...

  2. Dedifferentiated adipocyte-derived progeny cells (DFAT cells)

    OpenAIRE

    Wei, Shengjuan; Zan, Linsen; Hausman, Gary J.; Rasmussen, Theodore P; Bergen, Werner G.; Dodson, Michael V.

    2013-01-01

    Analyses of mature adipocytes have shown that they possess a reprogramming ability in vitro, which is associated with dedifferentiation. The subsequent dedifferentiated fat cells (DFAT cells) are multipotent and can differentiate into adipocytes and other cell types as well. Mature adipocytes can be easily obtained by biopsy, and the cloned progeny cells are homogeneous in vitro. Therefore, DFAT cells (a new type of stem cell) may provide an excellent source of cells for tissue regeneration, ...

  3. Comparison of f2/f1 ratio functions in rabbit and gerbil: Ear-canal DPOAEs vs noninvasively inferred intracochlear DPs

    Science.gov (United States)

    Martin, Glen K.; Stagner, Barden B.; Dong, Wei; Lonsbury-Martin, Brenda L.

    2015-12-01

    The properties of distortion product otoacoustic emissions (DPOAEs), i.e., distortion products (DPs) measured in the ear canal, have been thoroughly described. However, considerably less is known about the behavior of intracochlear DPs (iDPs). Detailed comparisons of DPOAEs to iDPs would provide valuable insights on the extent to which ear-canal DPOAEs mirror iDPs. Prior studies described a technique whereby the behavior of iDPs could be inferred by interacting a probe tone (f3) with the iDP of interest to produce a `secondary' DPOAE (DPOAÉ). The behavior of DPOAÉ was then used to deduce the characteristics of the iDP. In the present study, this method was used in rabbits and gerbils to simultaneously compare DPOAE f2/f1-ratio functions to their iDP counterparts. The 2f1-f2 and 2f2-f1 DPOAEs were collected with f1 and f2 primary-tone levels varied from 35-75 dB SPL, and with a 50-dB SPL f3 placed at a DP/f3 ratio of 1.25 to evoke a DPOAÉ at 2f3-(2f1-f2) or 2f3-(2f2-f1). Control experiments demonstrated little effect of the f3-probe tone on DPOAE-ratio functions. Substitution experiments were performed to determine any suppressive effects of the f1 and f2 primaries on the generation of DPOAÉ, as well as to infer the intracochlear level of the iDP once the DPOAÉ was corrected for suppression. Results showed that at low primary-tone levels, 2f1-f2 DPOAE f2/f1-ratio functions peaked around f2/f1=1.25, and exhibited an inverted U-shaped function. In contrast, simultaneously measured 2f1-f2 iDP-ratio functions peaked at f2/f1≈1. Similar growth of the inferred iDP was obtained for higher-level primaries when the ratio functions were corrected for suppressive effects. At these higher levels, DPOAE-ratio functions leveled off and no longer showed the steep reduction at narrow f2/f1 ratios. Overall, noninvasive estimates of 2f1-f2 iDP-ratio functions agreed with reports of similar functions directly measured for 2f1-f2 DPs on the basilar membrane (BM) or in

  4. Progeny Clustering: A Method to Identify Biological Phenotypes

    Science.gov (United States)

    Hu, Chenyue W.; Kornblau, Steven M.; Slater, John H.; Qutub, Amina A.

    2015-01-01

    Estimating the optimal number of clusters is a major challenge in applying cluster analysis to any type of dataset, especially to biomedical datasets, which are high-dimensional and complex. Here, we introduce an improved method, Progeny Clustering, which is stability-based and exceptionally efficient in computing, to find the ideal number of clusters. The algorithm employs a novel Progeny Sampling method to reconstruct cluster identity, a co-occurrence probability matrix to assess the clustering stability, and a set of reference datasets to overcome inherent biases in the algorithm and data space. Our method was shown successful and robust when applied to two synthetic datasets (datasets of two-dimensions and ten-dimensions containing eight dimensions of pure noise), two standard biological datasets (the Iris dataset and Rat CNS dataset) and two biological datasets (a cell phenotype dataset and an acute myeloid leukemia (AML) reverse phase protein array (RPPA) dataset). Progeny Clustering outperformed some popular clustering evaluation methods in the ten-dimensional synthetic dataset as well as in the cell phenotype dataset, and it was the only method that successfully discovered clinically meaningful patient groupings in the AML RPPA dataset. PMID:26267476

  5. {sup 220}Rn and its progeny in dwellings of Korea

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Chang-Kyu [International Atomic Energy Agency, Agency' s Laboratories, A-1400, Vienna (Austria)], E-mail: chang.kyu.kim@iaea.org; Kim, Young-Jae; Lee, Hwa-Yong; Chang, Byung-Uck [Korea Institute of Nuclear Safety, P.O. Box 114, Yusong, Daejeon 305-338 (Korea, Republic of); Tokonami, Shinji [Radon Research Group, National Institute of Radiological Sciences (Japan)

    2007-09-15

    Concentrations of {sup 220}Rn and its progeny were measured in 450 dwellings from 2002 to 2004 using two kinds of simple passive {sup 222}Rn and {sup 220}Rn monitors, Radopot{sup TM} and {sup 220}Rn progeny monitor. The annual arithmetic mean (AM) and geometric mean (GM) of {sup 220}Rn concentrations were 40.4 and 10.7Bqm{sup -3}, respectively. The annual arithmetic mean (AM) of EEC{sub Tn} concentration was 0.89{+-}0.70Bqm{sup -3}, and the annual geometric mean (GM) was 0.60Bqm{sup -3} (95% confidence interval: 0.43-0.78Bqm{sup -3}). The concentrations of {sup 220}Rn in Korean-style houses built with mud block were about two times higher than those in apartments. The average annual effective dose due to inhalation exposure to {sup 220}Rn and its progeny in dwellings was 0.25mSvy{sup -1}.

  6. Maternal Exposure to Cadmium and Manganese Impairs Reproduction and Progeny Fitness in the Sea Urchin Paracentrotus lividus

    Science.gov (United States)

    Migliaccio, Oriana; Castellano, Immacolata; Cirino, Paola; Romano, Giovanna; Palumbo, Anna

    2015-01-01

    Metal contamination represents one of the major sources of pollution in marine environments. In this study we investigated the short-term effects of ecologically relevant cadmium and manganese concentrations (10-6 and 3.6 x 10-5 M, respectively) on females of the sea urchin Paracentrotus lividus and their progeny, reared in the absence or presence of the metal. Cadmium is a well-known heavy metal, whereas manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. The effects of these agents were examined on both P. lividus adults and their offspring following reproductive state, morphology of embryos, nitric oxide (NO) production and differential gene expression. Here, we demonstrated that both metals differentially impaired the fertilization processes of the treated female sea urchins, causing modifications in the reproductive state and also affecting NO production in the ovaries. A detailed analysis of the progeny showed a high percentage of abnormal embryos, associated to an increase in the endogenous NO levels and variations in the transcriptional expression of several genes involved in stress response, skeletogenesis, detoxification, multi drug efflux processes and NO production. Moreover, we found significant differences in the progeny from females exposed to metals and reared in metal-containing sea water compared to embryos reared in non-contaminated sea water. Overall, these results greatly expanded previous studies on the toxic effects of metals on P. lividus and provided new insights into the molecular events induced in the progeny of sea urchins exposed to metals. PMID:26125595

  7. Maternal Exposure to Cadmium and Manganese Impairs Reproduction and Progeny Fitness in the Sea Urchin Paracentrotus lividus.

    Directory of Open Access Journals (Sweden)

    Oriana Migliaccio

    Full Text Available Metal contamination represents one of the major sources of pollution in marine environments. In this study we investigated the short-term effects of ecologically relevant cadmium and manganese concentrations (10(-6 and 3.6 x 10(-5 M, respectively on females of the sea urchin Paracentrotus lividus and their progeny, reared in the absence or presence of the metal. Cadmium is a well-known heavy metal, whereas manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. The effects of these agents were examined on both P. lividus adults and their offspring following reproductive state, morphology of embryos, nitric oxide (NO production and differential gene expression. Here, we demonstrated that both metals differentially impaired the fertilization processes of the treated female sea urchins, causing modifications in the reproductive state and also affecting NO production in the ovaries. A detailed analysis of the progeny showed a high percentage of abnormal embryos, associated to an increase in the endogenous NO levels and variations in the transcriptional expression of several genes involved in stress response, skeletogenesis, detoxification, multi drug efflux processes and NO production. Moreover, we found significant differences in the progeny from females exposed to metals and reared in metal-containing sea water compared to embryos reared in non-contaminated sea water. Overall, these results greatly expanded previous studies on the toxic effects of metals on P. lividus and provided new insights into the molecular events induced in the progeny of sea urchins exposed to metals.

  8. Maternal Exposure to Cadmium and Manganese Impairs Reproduction and Progeny Fitness in the Sea Urchin Paracentrotus lividus.

    Science.gov (United States)

    Migliaccio, Oriana; Castellano, Immacolata; Cirino, Paola; Romano, Giovanna; Palumbo, Anna

    2015-01-01

    Metal contamination represents one of the major sources of pollution in marine environments. In this study we investigated the short-term effects of ecologically relevant cadmium and manganese concentrations (10(-6) and 3.6 x 10(-5) M, respectively) on females of the sea urchin Paracentrotus lividus and their progeny, reared in the absence or presence of the metal. Cadmium is a well-known heavy metal, whereas manganese represents a potential emerging contaminant, resulting from an increased production of manganese-containing compounds. The effects of these agents were examined on both P. lividus adults and their offspring following reproductive state, morphology of embryos, nitric oxide (NO) production and differential gene expression. Here, we demonstrated that both metals differentially impaired the fertilization processes of the treated female sea urchins, causing modifications in the reproductive state and also affecting NO production in the ovaries. A detailed analysis of the progeny showed a high percentage of abnormal embryos, associated to an increase in the endogenous NO levels and variations in the transcriptional expression of several genes involved in stress response, skeletogenesis, detoxification, multi drug efflux processes and NO production. Moreover, we found significant differences in the progeny from females exposed to metals and reared in metal-containing sea water compared to embryos reared in non-contaminated sea water. Overall, these results greatly expanded previous studies on the toxic effects of metals on P. lividus and provided new insights into the molecular events induced in the progeny of sea urchins exposed to metals.

  9. Close phylogenetic relationship between Angolan and Romanian HIV-1 subtype F1 isolates

    Science.gov (United States)

    Guimarães, Monick L; Vicente, Ana Carolina P; Otsuki, Koko; da Silva, Rosa Ferreira FC; Francisco, Moises; da Silva, Filomena Gomes; Serrano, Ducelina; Morgado, Mariza G; Bello, Gonzalo

    2009-01-01

    Background Here, we investigated the phylogenetic relationships of the HIV-1 subtype F1 circulating in Angola with subtype F1 strains sampled worldwide and reconstructed the evolutionary history of this subtype in Central Africa. Methods Forty-six HIV-1-positive samples were collected in Angola in 2006 and subtyped at the env-gp41 region. Partial env-gp120 and pol-RT sequences and near full-length genomes from those env-gp41 subtype F1 samples were further generated. Phylogenetic analyses of partial and full-length subtype F1 strains isolated worldwide were carried out. The onset date of the subtype F1 epidemic in Central Africa was estimated using a Bayesian Markov chain Monte Carlo approach. Results Nine Angolan samples were classified as subtype F1 based on the analysis of the env-gp41 region. All nine Angolan sequences were also classified as subtype F1 in both env-gp120 and pol-RT genomic regions, and near full-length genome analysis of four of these samples confirmed their classification as "pure" subtype F1. Phylogenetic analyses of subtype F1 strains isolated worldwide revealed that isolates from the Democratic Republic of Congo (DRC) were the earliest branching lineages within the subtype F1 phylogeny. Most strains from Angola segregated in a monophyletic group together with Romanian sequences; whereas South American F1 sequences emerged as an independent cluster. The origin of the subtype F1 epidemic in Central African was estimated at 1958 (1934–1971). Conclusion "Pure" subtype F1 strains are common in Angola and seem to be the result of a single founder event. Subtype F1 sequences from Angola are closely related to those described in Romania, and only distantly related to the subtype F1 lineage circulating in South America. Original diversification of subtype F1 probably occurred within the DRC around the late 1950s. PMID:19386115

  10. Mechanistic Basis for Differential Inhibition of the F1Fo-ATPase by Aurovertin

    OpenAIRE

    2009-01-01

    The mitochondrial F1Fo-ATPase performs the terminal step of oxidative phosphorylation. Small molecules that modulate this enzyme have been invaluable in helping decipher F1Fo-ATPase structure, function, and mechanism. Aurovertin is an antibiotic that binds to the β subunits in the F1 domain and inhibits F1Fo-ATPase-catalyzed ATP synthesis in preference to ATP hydrolysis. Despite extensive study and the existence of crystallographic data, the molecular basis of the differential inhibition and ...

  11. Close phylogenetic relationship between Angolan and Romanian HIV-1 subtype F1 isolates

    Directory of Open Access Journals (Sweden)

    Serrano Ducelina

    2009-04-01

    Full Text Available Abstract Background Here, we investigated the phylogenetic relationships of the HIV-1 subtype F1 circulating in Angola with subtype F1 strains sampled worldwide and reconstructed the evolutionary history of this subtype in Central Africa. Methods Forty-six HIV-1-positive samples were collected in Angola in 2006 and subtyped at the env-gp41 region. Partial env-gp120 and pol-RT sequences and near full-length genomes from those env-gp41 subtype F1 samples were further generated. Phylogenetic analyses of partial and full-length subtype F1 strains isolated worldwide were carried out. The onset date of the subtype F1 epidemic in Central Africa was estimated using a Bayesian Markov chain Monte Carlo approach. Results Nine Angolan samples were classified as subtype F1 based on the analysis of the env-gp41 region. All nine Angolan sequences were also classified as subtype F1 in both env-gp120 and pol-RT genomic regions, and near full-length genome analysis of four of these samples confirmed their classification as "pure" subtype F1. Phylogenetic analyses of subtype F1 strains isolated worldwide revealed that isolates from the Democratic Republic of Congo (DRC were the earliest branching lineages within the subtype F1 phylogeny. Most strains from Angola segregated in a monophyletic group together with Romanian sequences; whereas South American F1 sequences emerged as an independent cluster. The origin of the subtype F1 epidemic in Central African was estimated at 1958 (1934–1971. Conclusion "Pure" subtype F1 strains are common in Angola and seem to be the result of a single founder event. Subtype F1 sequences from Angola are closely related to those described in Romania, and only distantly related to the subtype F1 lineage circulating in South America. Original diversification of subtype F1 probably occurred within the DRC around the late 1950s.

  12. TpF1 from Treponema pallidum activates inflammasome and promotes the development of regulatory T cells.

    Science.gov (United States)

    Babolin, Chiara; Amedei, Amedeo; Ozolins, Dzintars; Zilevica, Aija; D'Elios, Mario Milco; de Bernard, Marina

    2011-08-01

    Human syphilis is a multistage disease, with diverse and wide-ranging manifestations caused by Treponema pallidum. Despite the fact that a cell-mediated immune response takes part in the course of syphilis, T. pallidum often manages to evade host immunity and, in untreated individuals, may trigger chronic infection. With this study, we demonstrate for the first time, to our knowledge, that Treponema pallidum induces a regulatory T (Treg) response in patients with secondary syphilis and we found that the miniferritin TpF1, produced by the bacterium, is able to expand this response and promote the production of TGF-β. Accordingly, TpF1 stimulates monocytes to release IL-10 and TGF-β, the key cytokines in driving Treg cell differentiation. Interestingly, we also found that TpF1 stimulates monocytes to synthesize and release several proinflammatory cytokines, such as TNF-α, IL-6, and IL-1β, the latter following the activation of the multiprotein complex inflammasome. Collectively, these data strongly support a central role for TpF1 both in the inflammation process, which occurs in particular during the early stage of syphilis, and in the long-term persistence of the spirochete within the host by promoting Treg response and TGF-β production.

  13. 17 CFR 240.12f-1 - Applications for permission to reinstate unlisted trading privileges.

    Science.gov (United States)

    2010-04-01

    ... reinstate unlisted trading privileges. 240.12f-1 Section 240.12f-1 Commodity and Securities Exchanges... Rules and Regulations Under the Securities Exchange Act of 1934 Unlisted Trading § 240.12f-1 Applications for permission to reinstate unlisted trading privileges. (a) An application to reinstate...

  14. E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity

    Directory of Open Access Journals (Sweden)

    Fikret Sahin, Todd L. Sladek

    2010-01-01

    Full Text Available E2F-1 is the major cellular target of pRB and is regulated by pRB during cell proliferation. Interaction between pRB and E2F-1 is dependent on the phosphorylation status of pRB. Despite the fact that E2F-1 and pRB have antagonistic activities when they are overexpressed, the role of the E2F-1-pRB interaction in cell growth largely remains unknown. Ideally, it would be better to study the properties of a pRB mutant that fails to bind to E2F, but retains all other activities. To date, no pRB mutation has been characterized in sufficient detail to show that it specifically eliminates E2F binding but leaves other interactions intact. An alternative approach to this issue is to ask whether mutations that change E2F proteins binding affinity to pRB are sufficient to change cell growth in aspect of cell cycle and tumor formation. Therefore, we used the E2F-1 mutants including E2F-1/S332-7A, E2F-1/S375A, E2F-1/S403A, E2F-1/Y411A and E2F-1/L132Q that have different binding affinities for pRB to better understand the roles of the E2F-1 phosphorylation and E2F-1-pRB interaction in the cell cycle, as well as in transformation and gene expression. Data presented in this study suggests that in vivo phosphorylation at amino acids 332-337, 375 and 403 is important for the E2F-1 and pRB interaction in vivo. However, although E2F-1 mutants 332-7, 375 and 403 showed similar binding affinity to pRB, they showed different characteristics in transformation efficiency, G0 accumulation, and target gene experiments.

  15. Modulation of the E2F1-driven cancer cell fate by the DNA damage response machinery and potential novel E2F1 targets in osteosarcomas

    DEFF Research Database (Denmark)

    Liontos, Michalis; Niforou, Katerina; Velimezi, Georgia

    2009-01-01

    Osteosarcoma is the most common primary bone cancer. Mutations of the RB gene represent the most frequent molecular defect in this malignancy. A major consequence of this alteration is that the activity of the key cell cycle regulator E2F1 is unleashed from the inhibitory effects of pRb. Studies...... in animal models and in human cancers have shown that deregulated E2F1 overexpression possesses either "oncogenic" or "oncosuppressor" properties, depending on the cellular context. To address this issue in osteosarcomas, we examined the status of E2F1 relative to cell proliferation and apoptosis...... in a clinical setting of human primary osteosarcomas and in E2F1-inducible osteosarcoma cell line models that are wild-type and deficient for p53. Collectively, our data demonstrated that high E2F1 levels exerted a growth-suppressing effect that relied on the integrity of the DNA damage response network...

  16. Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding

    Directory of Open Access Journals (Sweden)

    Gous Miah

    2015-03-01

    Full Text Available The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast and Pongsu Seribu 1 (resistant to blast. The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers were found as polymorphic between the parents related to blast resistant genes (Pi-genes. Among the selected blast resistant linked markers, two markers RM6836 and RM8225 showed expected testcross ratio (1:1 for single-gene model in the BC2F1 population with the association between resistant and susceptible progeny. A total of 333-BC2F1 plants were challenged with the most virulent pathotype P7.2 of Magnaporthe oryzae. Chi-square (χ2 analysis for phenotypic segregation in single-gene model showed goodness of fit (P = 0.4463 to the expected segregation ratio (1:1. In marker segregation analysis, two polymorphic markers (RM6836 and RM8225 clearly showed goodness of fit to the expected segregation testcross ratio (1:1 for the single-gene model. The marker RM8225 and RM6836 showed significant R2 values higher than 10 for the trait of the blast lesions degree (BLD. The positions of RM6836 and RM8225 markers on rice chromosome 6 and the distance between these two markers is 0.2 cM. We conclude that single dominant gene control the blast resistance in Pongsu Seribu 1 located on chromosome 6, which is linked to RM8225 and RM6836 microsatellite markers. This information could be useful in marker-assisted selection for blast resistance in rice breeding involving Pongsu Seribu 1.

  17. Little qualitative RNA misexpression in sterile male F1 hybrids of Drosophila pseudoobscura and D. persimilis

    Directory of Open Access Journals (Sweden)

    Noor Mohamed AF

    2002-09-01

    Full Text Available Abstract Background Although the genetics of hybrid sterility has been the subject of evolutionary studies for over sixty years, no one has shown the reason(s why alleles that operate normally within species fail to function in another genetic background. Several lines of evidence suggest that failures in normal gene transcription contribute to hybrid dysfunctions, but genome-wide studies of gene expression in pure-species and hybrids have not been undertaken. Here, we study genome-wide patterns of expression in Drosophila pseudoobscura, D. persimilis, and their sterile F1 hybrid males using differential display. Results Over five thousand amplifications were analyzed, and 3312 were present in amplifications from both of the pure species. Of these, 28 (0.5% were not present in amplifications from adult F1 hybrid males. Using product-specific primers, we were able to confirm one of nine of the transcripts putatively misexpressed in hybrids. This transcript was shown to be male-specific, but without detectable homology to D. melanogaster sequence. Conclusion We tentatively conclude that hybrid sterility can evolve without widespread, qualitative misexpression of transcripts in species hybrids. We suggest that, if more misexpression exists in sterile hybrids, it is likely to be quantitative, tissue-specific, and/ or limited to earlier developmental stages. Although several caveats apply, this study was a first attempt to determine the mechanistic basis of hybrid sterility, and one potential candidate gene has been identified for further study.

  18. Response of resistant breeding lines of tomato germplasm and their progenies with Seedathip3 to Tomato Yellow Leaf Curl Virus, Thailand isolate (TYLCTHV-[2

    Directory of Open Access Journals (Sweden)

    Ornubol Chomdej

    2007-11-01

    Full Text Available Tomato germplasm accessions; FLA456-4, FLA591-15, H24, CLN2443A, CLN2443B, CLN2443C, TLB111, TLB182-1, TLB111-F6-4-1, TLB130-F6-3-1 and TLB134-F6-8-1 from the Asian Vegetable Research Development Center (AVRDC, Taiwan, were screened for resistance to the Tomato Yellow Leaf Curl Virus, Thailand isolate (TYLCTHV-[2]. The accessions expressing the resistant genotype were then crossed to the TYLCV-susceptible female parent, Seedathip3 (SD3, to produce F1 hybrids. Tomato parents and their F1 progenies were inoculated with TYLCTHV-[2] at 3 weeks of seedling age using viruliferous whitefly (Bemisia tabaci as the inoculation vector. Disease response of the seedling was rated according to the incidence and severity of the development of yellowing and curling symptoms. The presence of TYLCTHV-[2] in the inoculated plants was confirmed by Enzyme-Linked Immunosorbent Assay (ELISA. AVRDC tomato parental lines: H24, FLA591-15 and FLA456-4 expressed mild or no symptoms after one month inoculation. Progeny of crosses between the AVRDC donor parental lines and susceptible Thai cultivars showed intermediate tolerance to TYLCTHV-[2] infection. This indicated that resistance was incompletely dominant.

  19. F1大奖赛中国站与上海城市旅游发展%F1 Chinese Grand Prix and the Urban Tourism Development in Shanghai

    Institute of Scientific and Technical Information of China (English)

    马洁; 黄嬛; 黄海燕

    2011-01-01

    On the basis of stating the inner consistency of sports events and urban tourism,the paper gives a brief introduction of F1 Grand Prix and urban tourism in some foreign countries.It sums up the role of F1 Chinese Grand Prix on the development of urban tourism in Shanghai.The event accelerates the economic development of the tourism-related industries in Shanghai,strengthens the tourism interaction between Shanghai and its neighboring regions,enriches the varieties of Shanghai tourist products and betters the image of tourism in Shanghai.%在理论分析了体育赛事和城市旅游业内在一致性的基础上,介绍了国外F1大奖赛与城市旅游概况。概述了F1大奖赛中国站对上海城市旅游发展的作用:带动了上海旅游相关产业的经济发展;加强上海与周边区域的旅游互动;丰富了上海旅游产品的种类;改善了上海市旅游形象。

  20. Produção de Madeira de Progênies de Segunda Geração de Grevílea na Região de Avaré, SP Second Generation Half-sib Progenies Wood Production of Grevillea in the Region of Avaré - SP

    Directory of Open Access Journals (Sweden)

    Emerson Gonçalves Martins

    2011-03-01

    Full Text Available

    A grevílea (Grevillea robusta A. Cunn é uma espécie utilizada para o sombreamento de cafezais e de outras culturas agrícolas, áreas de pastagens e apicultura, paisagismo e como cortina quebra- ventos. Sua madeira também é utilizada para desdobro, em serrarias. Um teste com 28 progênies de meios-irmãos de segunda geração foi instalado no Município de Avaré, São Paulo. O objetivo principal foi avaliar e selecionar o material genético mais produtivo em volume de madeira. Dados de altura e diâmetro à altura do peito (DAP foram coletados na área experimental, na idade de três anos. Baseando-se nos dados estimados de volume cilíndrico com casca, as seguintes conclusões foram obtidas: em Avaré, SP, a progênie de segunda geração mais produtiva e com maior taxa de sobrevivência pertencente à procedência australiana de Boyd River (NSW. A progênie de segunda geração mais produtiva, em termos de volume cilíndrico de madeira com casca, foi 138 % superior à media geral do teste. Considerando o valor médio de volume de madeira para apenas as doze progênies de segunda geração mais produtivas, foi possível observar um aumento de 32 % em relação à média geral do experimento. 
    The Grevillea robusta A. Cunn is utilized for coffee plantation shading, crops growing and pasture shading, apiculture, ornamentation and wind breaks. It is also used to produce sawn wood. One test of 28 second generation half-sib progenies was planted in Forestry Institute of São Paulo, with 20°03’S latitude and 48°54’W logitude geographic coordenates and 630m of altitude. The main objectives of this paper were to select genetic material capable to produce more volume of wood. The total heigth and diameter at breast height (DBH were measured on the three yars old second generation half-sib progenies test. The volume was calculated and the results led to the following conclusion: The best

  1. Genetic determinism of anatomical and hydraulic traits within an apple progeny.

    Science.gov (United States)

    Lauri, Pierre-Éric; Gorza, Olivier; Cochard, Hervé; Martinez, Sébastien; Celton, Jean-Marc; Ripetti, Véronique; Lartaud, Marc; Bry, Xavier; Trottier, Catherine; Costes, Evelyne

    2011-08-01

    The apple tree is known to have an isohydric behaviour, maintaining rather constant leaf water potential in soil with low water status and/or under high evaporative demand. However, little is known on the xylem water transport from roots to leaves from the two perspectives of efficiency and safety, and on its genetic variability. We analysed 16 traits related to hydraulic efficiency and safety, and anatomical traits in apple stems, and the relationships between them. Most variables were found heritable, and we investigated the determinism underlying their genetic control through a quantitative trait loci (QTL) analysis on 90 genotypes from the same progeny. Principal component analysis (PCA) revealed that all traits related to efficiency, whether hydraulic conductivity, vessel number and area or wood area, were included in the first PC, whereas the second PC included the safety variables, thus confirming the absence of trade-off between these two sets of traits. Our results demonstrated that clustered variables were characterized by common genomic regions. Together with previous results on the same progeny, our study substantiated that hydraulic efficiency traits co-localized with traits identified for tree growth and fruit production.

  2. A Sequential Quantitative Trait Locus Fine-Mapping Strategy Using Recombinant-Derived Progeny

    Institute of Scientific and Technical Information of China (English)

    Qin Yang; Dongfeng Zhang; Mingliang Xu

    2012-01-01

    A thorough understanding of the quantitative trait loci (QTLs) that underlie agronomically important traits in crops would greatly increase agricultural productivity.Although advances have been made in QTL cloning,the majority of QTLs remain unknown because of their low heritability and minor contributions to phenotypic performance.Here we summarize the key advantages and disadvantages of current QTL fine-mapping methodologies,and then introduce a sequential QTL fine-mapping strategy based on both genotypes and phenotypes of progeny derived from recombinants.With this mapping strategy,experimental errors could be dramatically diminished so as to reveal the authentic genetic effect of target QTLs.The number of progeny required to detect QTLs at various R2 values was calculated,and the backcross generation suitable to start QTL fine-mapping was also estimated.This mapping strategy has proved to be very powerful in narrowing down QTL regions,particularly minor-effect QTLs,as revealed by fine-mapping of various resistance QTLs in maize.Application of this sequential QTL mapping strategy should accelerate cloning of agronomically important QTLs,which is currently a substantial challenge in crops.

  3. The regional lung deposition of thoron progeny attached to the particulate phase of environmental tobacco smoke

    Energy Technology Data Exchange (ETDEWEB)

    Strong, J.C.; Black, A.; Knight, D.A.; Dickens, C.J.; McAughey, J. (AEA Technology, Harwell (United Kingdom))

    1994-01-01

    The [gamma] emitting isotope of lead (([sup 212]Pb), a decay product of thoron ([sup 220]Rn)), has been used to radiolabel the particulate phase of aged and diluted sidestream tobacco smoke in situ. The radiolabelled aerosol is suitable for studies with human volunteers and as a marker for lung deposition and clearance of the attached fraction of thoron progeny, as well as environmental tobacco smoke (ETS) in a variety of environmental situations. Total mean deposition values for nine male volunteers varied from 22% to 59% under different breathing conditions, including nasal as against mouth breathing. These data are higher than previously reported values for environmental tobacco smoke but are consistent with models of particle deposition in the lung. Data for regional deposition and clearance show deposition principally in the pulmonary region of the lung. Preliminary studies using radon ([sup 222]Rn) to determine the degree of association of progeny in the presence of ambient particulate or environmental tobacco smoke confirm that the degree of attachment rises with increasing particle concentration, and is therefore likely to influence both the magnitude and site of deposition in the lung. (author).

  4. The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease

    Directory of Open Access Journals (Sweden)

    Abdelmonim Ali Ahmad

    2014-07-01

    Full Text Available In this study, filamentous phage XacF1, which can infect Xanthomonas axonopodis pv. citri (Xac strains, was isolated and characterized. Electron microscopy showed that XacF1 is a member of the family Inoviridae and is about 1000 nm long and 16 nm in width. The genome of XacF1 is 7325 nucleotides in size, containing 13 predicted open reading frames (ORFs, some of which showed significant homology to Ff-like phage proteins such as ORF1 (pII, ORF2 (pV, ORF6 (pIII, and ORF8 (pVI. XacF1 showed a relatively wide host range, infecting seven out of 11 strains tested in this study. Frequently, XacF1 was found to be integrated into the genome of Xac strains. This integration occurred at the host dif site (attB and was mediated by the host XerC/D recombination system. The attP sequence was identical to that of Xanthomonas phage Cf1c. Interestingly, infection by XacF1 phage caused several physiological changes to the bacterial host cells, including lower levels of extracellular polysaccharide production, reduced motility, slower growth rate, and a dramatic reduction in virulence. In particular, the reduction in virulence suggested possible utilization of XacF1 as a biological control agent against citrus canker disease.

  5. Dale Reed with model in front of M2-F1

    Science.gov (United States)

    1967-01-01

    Briegleb Glider Company. The budget was $30,000. NASA craftsmen and engineers built the tubular steel interior frame. Its mahogany plywood shell was hand-made by Gus Briegleb and company. Ernie Lowder, a NASA craftsman who had worked on the Howard Hughes 'Spruce Goose,' was assigned to help Briegleb. The prototype of a 21st Century spacecraft required the fabrication of hundreds of small wooden parts meticulously nailed and glued together. It was a product of craftsmanship that was nearly obsolete in the 1940s. Final assembly of the remaining components (including aluminum tail surfaces, push rod controls, and landing gear from a Cessna 150) was done back at the NASA facility. In the meantime, other NASA engineers devised a special M2-F1 flight simulator, and a hot rod shop near Long Beach souped-up a Pontiac convertible to be used as the lifting body ground-tow vehicle. The M2-F1 did not have ailerons. Instead, it had elevons which were attached to each of the two rudders. A large flap on the trailing edge of the body acted as an elevator. This unconventional arrangement prompted the engineers to rethink the flight control system as well. They eventually devised two schemes. One system was fairly traditional. It used rudder pedal inputs to move the rudders for yaw control, and stick inputs to provide differential deflections of the elevons for roll. The other system used stick inputs to control the rudders for yaw, while rudder pedal deflections moved the elevons for roll. Milt Thompson tried both systems in the simulator and surprised the design team when he said he preferred system number two. He reasoned that although sideslip delayed roll (which was a result of dihedral effect), the roll rate was twice as high using the rudders instead of the elevons. He said he would rather have the higher roll rates available to him if needed, while the slip could be overcome with proper piloting technique. This was the system that Thompson practiced on the simulator, and he used it

  6. A recombinant raccoon poxvirus vaccine expressing both Yersinia pestis F1 and truncated V antigens protects animals against lethal plague.

    Science.gov (United States)

    Rocke, Tonie E.; Kingstad-Bakke, B; Berlier, W; Osorio, J.E.

    2014-01-01

    In previous studies, we demonstrated in mice and prairie dogs that simultaneous administration of two recombinant raccoon poxviruses (rRCN) expressing Yersinia pestis antigens (F1 and V307-a truncated version of the V protein) provided superior protection against plague challenge compared to individual single antigen constructs. To reduce costs of vaccine production and facilitate implementation of a sylvatic plague vaccine (SPV) control program for prairie dogs, a dual antigen construct is more desirable. Here we report the construction and characterization of a novel RCN-vectored vaccine that simultaneously expresses both F1 and V307 antigens. This dual antigen vaccine provided similar levels of protection against plague in both mice and prairie dogs as compared to simultaneous administration of the two single antigen constructs and was also shown to protect mice against an F1 negative strain of Y. pestis.. The equivalent safety, immunogenicity and efficacy profile of the dual RCN-F1/V307 construct warrants further evaluation in field efficacy studies in sylvatic plague endemic areas.

  7. Correlation Analysis between Body Size and Slaughter Performance in F-1 Hybrid Offspring of Princess Chicken and Kirin Chicken

    Institute of Scientific and Technical Information of China (English)

    Li; Naibin; Du; Bingwang; Yang; Fenxia; Tao; Lin; Chen; Jiebo

    2014-01-01

    In order to study the meat development value of princess chicken,the body size traits and slaughter performance of 12-week-old F1 hybrid offspring of princess chicken(♂) and kirin chicken(♀) were measured and the correlations between different traits were analyzed. The results showed that body length,keel length and shank length of male F1 hybrid offspring were significantly higher than those of female chickens(P < 0. 05). The live weight,carcass weight,semi-eviscerated weight,semi-eviscerated ratio,eviscerated weight,chest muscle weight,the leg muscle weight and heart weight of male chickens were extremely significantly higher than that of female chickens(P < 0. 01),and the leg muscle ratio and wings weight were significantly higher than that of female chickens(P < 0. 05),but sebum thickness of male chickens was extremely significantly lower than that of female chickens(P < 0. 01). Other indicators failed to reach the significant difference level. There were extremely significant or significant correlations between the slaughter performance and body size in F1 hybrid offspring. The regression equations between different indicators were identified and developed. The results provided a certain theoretical reference to predict slaughter performance indicators through a living body size measurement,and revealed an improved production performance of F1 hybrid offspring.

  8. Production of a primary trisomic from progeny of cabbage-Chinese cabbage allotriploid backcrossing with cabbage%结球甘蓝-大白菜异源三倍体与结球甘蓝回交获得初级三体的研究

    Institute of Scientific and Technical Information of China (English)

    李艳霞; 管志坤; 赵建军; 申书兴; 王彦华

    2012-01-01

    In this study, an aneuploid plant with 2n + l chromosome was obtained from the progeny of cabbage-Chinese cabbage allotriploid backcrossing with cabbage. Cytological and SSR analysis showed that the aneuploid plant was a primary trisomic - 8 of cabbage. Furthermore, the chromosome number of the self ing and backcrossing progenies of the primary triso-mic-8 plant was identified, and the transfer rate of female and male gametes of extra-chromosome was clarified as 34. 74% and 6. 17%, respectively.%采用结球甘蓝-大白菜异源三倍体与二倍体结球甘蓝回交的方式,从回交后代BC2中获得了1株2n+1非整倍体植株.经细胞学和SSR鉴定分析,确认该2n+1植株为结球甘蓝8号初级三体.通过对8号初级三体植株自交和回交后代染色体数目的测定,明确了其额外染色体雌、雄配子的传递率分别为34.74%和6.17%.

  9. Carcinogenicity study of cochineal in B6C3F1 mice.

    Science.gov (United States)

    Mori, H; Iwata, H; Tanaka, T; Morishita, Y; Mori, Y; Kojima, T; Okumura, A

    1991-09-01

    The carcinogenicity of cochineal, a red colouring used in food and other products, was studied in a 2-yr bioassay in B6C3F1 mice. Groups of 50-55 mice of each sex were given 0, 3 or 6% cochineal in the diet for 2 yr. Mice of all groups developed tumours including hepatocellular adenomas or carcinomas, pulmonary adenomas or adenocarcinomas and lymphomas or lymphatic leukaemias, and the incidences of these tumours were not significantly different in treated and control groups. The results indicate that cochineal lacks carcinogenicity in mice and are consistent with those of in vitro short-term assays of cochineal and of carminic acid, an active principle of cochineal.

  10. Performance of Nellore and F1 (Red Angus x Nellore raised on pasture in southern Bahia

    Directory of Open Access Journals (Sweden)

    Amauri Arias Wenceslau

    2010-02-01

    Full Text Available Twenty Nelore and twenty F1 cattle (½ Red Angus, ½ Nelore, average age 12 months were compared in pastured raised at southern Bahia, in relation to production characteristics: initial weight (IW, final weight (FW, weight gain, average daily gain (ADG and total weight gain (WG, infection by endoparasites and heat tolerance (HT. To analyze the effect of genetic group, it was stabilished effects that could influence these characteristics and performed variance analysis using the program SAS. The results showed that IW, FW, ADG and WG of crossbred were statistically different in comparison to zebu cattle. Related to infection by endoparasites and heat tolerance there were no statistical differences between genetic groups.

  11. Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line

    DEFF Research Database (Denmark)

    Saito, M; Helin, K; Valentine, M B;

    1995-01-01

    and overexpressed in HEL erythroleukemia cells and translocated to other chromosomes in several established human leukemia cell lines. This study provides the first evidence of gene amplification involving a member of the E2F family of transcription factors. We propose that E2F1 overexpression in erythroid......The E2F transcription factor plays an important regulatory role in cell proliferation, mediating the expression of genes whose products are essential for inducing resting cells to enter the cell cycle and synthesize DNA. To investigate the possible involvement of E2F in hematopoietic malignancies...... progenitors may stimulate abnormal cell proliferation by overriding negative regulatory signals mediated by tumor suppressor proteins such as pRb....

  12. The nonlinear chemo-mechanic coupled dynamics of the F 1 -ATPase molecular motor.

    Science.gov (United States)

    Xu, Lizhong; Liu, Fang

    2012-03-01

    The ATP synthase consists of two opposing rotary motors, F0 and F1, coupled to each other. When the F1 motor is not coupled to the F0 motor, it can work in the direction hydrolyzing ATP, as a nanomotor called F1-ATPase. It has been reported that the stiffness of the protein varies nonlinearly with increasing load. The nonlinearity has an important effect on the rotating rate of the F1-ATPase. Here, considering the nonlinearity of the γ shaft stiffness for the F1-ATPase, a nonlinear chemo-mechanical coupled dynamic model of F1 motor is proposed. Nonlinear vibration frequencies of the γ shaft and their changes along with the system parameters are investigated. The nonlinear stochastic response of the elastic γ shaft to thermal excitation is analyzed. The results show that the stiffness nonlinearity of the γ shaft causes an increase of the vibration frequency for the F1 motor, which increases the motor's rotation rate. When the concentration of ATP is relatively high and the load torque is small, the effects of the stiffness nonlinearity on the rotating rates of the F1 motor are obvious and should be considered. These results are useful for improving calculation of the rotating rate for the F1 motor and provide insight about the stochastic wave mechanics of F1-ATPase.

  13. Comparison of outdoor activity size distributions of {sup 220}Rn and {sup 222}Rn progeny

    Energy Technology Data Exchange (ETDEWEB)

    Mohamed, A. [Physics Department, Faculty of Science, El-Minia University (Egypt)]. E-mail: amermohamed6@hotmail.com; El-Hussein, A. [Physics Department, Faculty of Science, El-Minia University (Egypt)

    2005-06-01

    Inhalation of {sup 222}Rn and {sup 220}Rn progeny from the domestic environment contributes the greatest fraction of the natural radiation exposure to the public. Dosimetric models are most often used in the assessment of human lung doses due to inhaled radioactivity because of the difficulty in making direct measurements. These models require information about the parameters of activity size distributions of thoron and radon progeny. The present study presents measured data on the attached and unattached activity size distributions of thoron and radon progeny in outdoor air in El-Minia, Egypt. The attached fraction was collected using a low-pressure Berner cascade impactor technique. A screen diffusion battery was used for collecting the unattached fraction. Most of the attached activities for {sup 222}Rn and {sup 220}Rn progeny were associated with aerosol particles of the accumulation mode. The activity size distribution of thoron progeny was found to be shifted to slightly smaller particle size compared to radon progeny.

  14. Mate choice and polyandry benefit reproduction and progeny fitness in the ladybird Hippodamia variegata (Goeze

    Directory of Open Access Journals (Sweden)

    Ahmad Pervez

    2011-12-01

    Full Text Available The effect of mate choice and polyandry on the reproductive performance and progeny fitness of a predaceous ladybird, Hippodamia variegata Goeze, was studied. The following mating combinations were used: (i virgin female paired with the same and initially unmated male once per day (monogamy with limited mating, (ii virgin female paired once daily with an unmated male (polyandry with no mate choice and (iii virgin female kept with five unmated males (polyandry with mate choice, with the food in each case an ad libitum supply of Aphis craccivora. Polyandrous females that had a free choice of mates were significantly more fecund (585.50 ± 13.80 eggs and higher percentage of their eggs hatched (95.32 ± 1.99% than was the case for polyandrous females with no choice of mates (495.20 ± 14.25 eggs and 88.81 ± 3.29% followed by monogamous females (405.00 ± 16.20 eggs and 83.13 ± 2.07%. Thus, mate choice and multiple mating by females is advantageous in terms of progeny production. The larvae that hatched from the eggs laid by these females were reared to maturity and their survival, duration of development and fitness determined. The fitness of offspring of polyandrous females (0.4787 ± 0.01 was better than that of polyandrous females that did not choose their mates (0.4418 ± 0.02 and monogamous (0.4057 ± 0.01 females. In addition, a greater percentage of the offspring of polyandrous females that chose their mates survived and developed faster than those of monogamous females with limited mating. These results reveal that polyandry with mate choice results in increased reproduction and better offspring quality. Hence, if one is interested increasing the production of H. variegata it is recommended that systems of production are developed that enable the females to be polyandrous and to select their mates.

  15. Sudetic larch in Germany - Results of provenance and progeny research

    Energy Technology Data Exchange (ETDEWEB)

    Weisgerber, H. [Forest Centre for Management Planning, Research and Ecology, Hann Muenden (Germany)

    1995-12-31

    There are only a few older sources of Sudetic larch in Germany. They distinguish themselves by outstanding growth and low susceptibility to canker. This impression was confirmed by results of provenance research. The Sudetic larch tested in comparison with numerous other provenances proved to be fast-growing, site-tolerant, to a large extent insusceptible to canker, with straight but also slightly to moderately curved stems. The Sudetic provenances behave remarkably uniformly as regards these characteristics. In addition to provenance research investigations have been going on for a long time in Germany into individual differences within the Sudetic larch populations. A report is given on the results of progeny tests from free and controlled pollination, using the example of a seed orchard consisting of 54 clones. We point also to possibilities for improving stem quality by selection steps. The results of provenance and progeny research on Sudetic larch are in the meantime being put to use to a large extent in practical forestry. The forest administrations of various federal lands recommend the use of reproductive material of Sudetic origin and from seed orchards. 20 refs, 3 figs

  16. New tools for radon progeny hazard prevention in mines

    Energy Technology Data Exchange (ETDEWEB)

    Skowronek, J.; Kajdasz, R.; Strzesniewicz, Z. [Central Mining Institute, Katowice (Poland). Laboratory of Radiometry

    2001-07-01

    The described project is an example of prevention actions performed under real conditions in one of Polish coal mine. This project, titled Prevention Of The Short Lived Radon Progeny Hazard was carried out by 'Nadwislanska Splka Weglowa' S.A., 'Ziemowit' Colliery and Central Mining Institute. The scientific part of the research was financed by Scientific Research Committee in Warsaw and the institutional part by Mining Plant 'Ziemowit'. The aim of the project was to give the mining industry a set of instruments what would enable it to influence the level of the radon progeny hazard on every stage of work, starting from planning the exploitation, prevention of radon secretion from orogene to the elimination of radon from the air by means of filtration. The project was completed successfully and its results have been instituted in Nadwislanska Splka Weglowa S.A. and Mining Plant 'Ziemowit'. The research has been made during 1994-6, and investments during 1996-7. 8 refs., 1 tab.

  17. Unattached fraction of radon progeny in Polish coal mines

    Energy Technology Data Exchange (ETDEWEB)

    Skubacz, K.; Chalupnik, S. [Central Mining Institute, Katowice (Poland). Laboratory of Radiometry

    2001-07-01

    The system of the monitoring of the radiation hazard in Polish underground mines is based on the monitoring of the workplaces. As the routine instrument, a device called ALFA-31is used to measure the radon progeny concentration in air. This unit proceeds together with the cyclone, the separator of the respirable fraction in the dust sampler. The cyclone causes the cut-off of unattached fraction of the short lived radon progeny that can play a very important role in the investigations of the adequate dose from this source of radiation hazard. Measurements of the unattached fraction were done with help of the alpha spectroscopy system and special kind of metal grids called diffusion battery screens that are designated to separate the unattached fraction. Results of the survey show that the average ratio of unattached to attached fractions is at level 3-5% in Polish coal mines. But it may cause the significant increase in the dose equivalent, due to these calculations at least 15-20% in comparison with dose equivalent caused by attached fraction 13 refs., 3 figs., 4 tabs.

  18. Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower

    Directory of Open Access Journals (Sweden)

    Terzić Sreten

    2006-01-01

    Full Text Available Phenotype, chromosomes pairing and pollen vitality were compared between parental populations and F1 hybrids of interspecific cross between Helianthus annuus L. and cultivated sunflower. The investigation of the simple sequence repeats (SSR polymorphism was also used to test the hybrid nature of F1 populations. The phenotypic traits of F1 hybrid plants were either closer to the wild species or intermediate. Irregular chromosome pairing was found in only 0 to 10% of meiocytes in the meiosis of F1 hybrid plants. Interspecific crosses were confirmed with SSR markers in all hybrid combinations. Alleles that were not present in parental DNA were frequently observed in F1 hybrids. That is additional evidence that those hybrid combinations were not produced by self-fertilization. The results suggest that SSR markers can be efficiently used for the F1 hybrid characterization in crosses between closely related species, in which, the changes of phenotype, meiosis and pollen vitality are not always significant.

  19. THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE

    Directory of Open Access Journals (Sweden)

    Satoto Satoto

    2016-10-01

    Full Text Available Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2 of fivetransgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. Data  resulted from PCR of F2 population were analysed using a Chi Square test. The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of genetic sources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer or  separately in rice breeding programs whenever

  20. The tumor suppressor gene hypermethylated in cancer 1 is transcriptionally regulated by E2F1

    DEFF Research Database (Denmark)

    Jenal, Mathias; Trinh, Emmanuelle; Britschgi, Christian;

    2009-01-01

    The Hypermethylated in Cancer 1 (HIC1) gene encodes a zinc finger transcriptional repressor that cooperates with p53 to suppress cancer development. We and others recently showed that HIC1 is a transcriptional target of p53. To identify additional transcriptional regulators of HIC1, we screened...... to the HIC1 promoter was shown by chromatin immunoprecipitation assays in human TIG3 fibroblasts expressing tamoxifen-activated E2F1. In agreement, activation of E2F1 in TIG3-E2F1 cells markedly increased HIC1 expression. Interestingly, expression of E2F1 in the p53(-/-) hepatocellular carcinoma cell line...

  1. E. coli F1-ATPase: site-directed mutagenesis of the beta-subunit.

    Science.gov (United States)

    Parsonage, D; Wilke-Mounts, S; Senior, A E

    1988-05-09

    Residues beta Glu-181 and beta Glu-192 of E. coli F1-ATPase (the DCCD-reactive residues) were mutated to Gln. Purified beta Gln-181 F1 showed 7-fold impairment of 'unisite' Pi formation from ATP and a large decrease in affinity for ATP. Thus the beta-181 carboxyl group in normal F1 significantly contributes to catalytic site properties. Also, positive catalytic site cooperativity was attenuated from 5 X 10(4)- to 548-fold in beta Gln-181 F1. In contrast, purified beta Gln-192 F1 showed only 6-fold reduction in 'multisite' ATPase activity. Residues beta Gly-149 and beta Gly-154 were mutated to Ile singly and in combination. These mutations, affecting residues which are strongly conserved in nucleotide-binding proteins, were chosen to hinder conformational motion in a putative 'flexible loop' in beta-subunit. Impairment of purified F1-ATPase ranged from 5 to 61%, with the double mutant F1 less impaired than either single mutant. F1 preparations containing beta Ile-154 showed 2-fold activation after release from membranes, suggesting association with F0 restrained turnover on F1 in these mutants.

  2. Clustering of conformational IgE epitopes on the major dog allergen Can f 1.

    Science.gov (United States)

    Curin, Mirela; Weber, Milena; Hofer, Gerhard; Apostolovic, Danijela; Keller, Walter; Reininger, Renate; Swoboda, Ines; Spitzauer, Susanne; Focke-Tejkl, Margit; van Hage, Marianne; Valenta, Rudolf

    2017-09-22

    Immunoglobulin E (IgE)-associated allergy affects more than 25% of the population. Can f 1 is the major dog allergen associated with respiratory symptoms but the epitopes recognized by allergic patients IgE on Can f 1 are unknown. To characterize IgE epitopes of Can f 1 recognized by dog allergic patients, six overlapping peptides spanning the Can f 1 sequence were synthesized. In direct IgE epitope mapping experiments peptides were analyzed for IgE reactivity by dot blot and Enzyme-linked immunosorbent assay (ELISA) with sera from dog allergic patients. For indirect epitope-mapping, rabbits were immunized with the peptides to generate specific IgG antibodies which were used to inhibit allergic patients' IgE binding to Can f 1. IgE binding sites were visualized on a model of the Can f 1 three-dimensional structure. We found that Can f 1 does not contain any relevant sequential IgE epitopes. However, IgE inhibition experiments with anti-peptide specific IgGs showed that Can f 1 N- and C-terminal portion assembled a major conformational binding site. In conclusion, our study is the first to identify the major IgE epitope-containing area of the dog allergen Can f 1. This finding is important for the development of allergen-specific treatment strategies.

  3. E2F1 transcription factor and its impact on growth factor and cytokine signaling.

    Science.gov (United States)

    Ertosun, Mustafa Gokhan; Hapil, Fatma Zehra; Osman Nidai, Ozes

    2016-10-01

    E2F1 is a transcription factor involved in cell cycle regulation and apoptosis. The transactivation capacity of E2F1 is regulated by pRb. In its hypophosphorylated form, pRb binds and inactivates DNA binding and transactivating functions of E2F1. The growth factor stimulation of cells leads to activation of CDKs (cyclin dependent kinases), which in turn phosphorylate Rb and hyperphosphorylated Rb is released from E2F1 or E2F1/DP complex, and free E2F1 can induce transcription of several genes involved in cell cycle entry, induction or inhibition of apoptosis. Thus, growth factors and cytokines generally utilize E2F1 to direct cells to either fate. Furthermore, E2F1 regulates expressions of various cytokines and growth factor receptors, establishing positive or negative feedback mechanisms. This review focuses on the relationship between E2F1 transcription factor and cytokines (IL-1, IL-2, IL-3, IL-6, TGF-beta, G-CSF, LIF), growth factors (EGF, KGF, VEGF, IGF, FGF, PDGF, HGF, NGF), and interferons (IFN-α, IFN-β and IFN-γ).

  4. Oncolytic reovirus induces intracellular redistribution of Ras to promote apoptosis and progeny virus release.

    Science.gov (United States)

    Garant, K A; Shmulevitz, M; Pan, L; Daigle, R M; Ahn, D-G; Gujar, S A; Lee, P W K

    2016-02-11

    Reovirus is a naturally oncolytic virus that preferentially replicates in Ras-transformed cells and is currently undergoing clinical trials as a cancer therapeutic. Ras transformation promotes reovirus oncolysis by enhancing virion disassembly during entry, viral progeny production, and virus release through apoptosis; however, the mechanism behind the latter is not well understood. Here, we show that reovirus alters the intracellular location of oncogenic Ras to induce apoptosis of H-RasV12-transformed fibroblasts. Reovirus infection decreases Ras palmitoylation levels and causes accumulation of Ras in the Golgi through Golgi fragmentation. With the Golgi being the site of Ras palmitoylation, treatment of target cells with the palmitoylation inhibitor, 2-bromopalmitate (2BP), prompts a greater accumulation of H-RasV12 in the Golgi, and a dose-dependent increase in progeny virus release and subsequent spread. Conversely, tethering H-RasV12 to the plasma membrane (thereby preventing its movement to the Golgi) allows for efficient virus production, but results in basal levels of reovirus-induced cell death. Analysis of Ras downstream signaling reveals that cells expressing cycling H-RasV12 have elevated levels of phosphorylated JNK (c-Jun N-terminal kinase), and that Ras retained at the Golgi body by 2BP increases activation of the MEKK1/MKK4/JNK signaling pathway to promote cell death. Collectively, our data suggest that reovirus induces Golgi fragmentation of target cells, and the subsequent accumulation of oncogenic Ras in the Golgi body initiates apoptotic signaling events required for virus release and spread.

  5. Acrylonitrile is a multisite carcinogen in male and female B6C3F1 mice.

    Science.gov (United States)

    Ghanayem, Burhan I; Nyska, Abraham; Haseman, Joseph K; Bucher, John R

    2002-07-01

    Acrylonitrile is a heavily produced unsaturated nitrile, which is used in the production of synthetic fibers, plastics, resins, and rubber. Acrylonitrile is a multisite carcinogen in rats after exposure via gavage, drinking water, or inhalation. No carcinogenicity studies of acrylonitrile in a second animal species were available. The current studies were designed to assess the carcinogenicity of acrylonitrile in B6C3F1 mice of both sexes. Acrylonitrile was administered by gavage at 0, 2.5, 10, or 20 mg/kg/day, 5 days per week, for 2 years. Urinary thiocyanate and N-acetyl-S-(2-cyanoethyl)-L-cysteine were measured as markers of exposure to acrylonitrile. In general, there were dose-related increases in urinary thiocyanate and N-acetyl-S-(2-cyanoethyl)-L-cysteine concentrations in all dosed groups of mice and at all time points. Survival was significantly (p acrylonitrile-dosed groups. In female mice, the incidence of benign or malignant granulosa cell tumors (combined) in the ovary in the 10 mg/kg dose group was greater than that in the vehicle control group, but because of a lack of dose response, this was considered an equivocal finding. In addition, the incidences of atrophy and cysts in the ovary of the 10 and 20 mg/kg dose groups were significantly increased. The incidences of alveolar/bronchiolar adenoma or carcinoma (combined) were significantly increased in female mice treated with acrylonitrile at 10 mg/kg/day for 2 years. This was also considered an equivocal result. In conclusion, these studies demonstrated that acrylonitrile causes multiple carcinogenic effects after gavage administration to male and female B6C3F1 mice for 2 years.

  6. Carcinogenicity of bisphenol-A in Fischer rats and B6C3F1 mice.

    Science.gov (United States)

    Huff, J

    2001-11-01

    Bisphenol-A (BP-A; 4,4'-isopropylidenediphenol) is a monomer of plastics commonly used in various consumer products, and is used as an intermediate in the manufacture of epoxy, polycarbonate, and polyester-styrene resins. A National Toxicology Program carcinogenesis bioassay of BP-A (>98% pure) was conducted by feeding diets containing 0, 1000, or 2000 ppm BP-A to groups of 50 male and 50 female Fischer (F)344 rats; 0, 1000, or 5000 ppm to groups of 50 male B6C3F1 mice; and 0, 5000, or 10,000 ppm to groups of 50 female B6C3F1 mice for 103 weeks. The mean body weights of the low- and high-dose rats and of female mice and high-dose male mice were lower than those of the controls throughout much of the study. Lower body weight gains in rats were likely caused by reduced food consumption. Survivals were comparable among groups. Regarding neoplasia, leukemias occurred at increased incidences in BP-A-dosed rats of both sexes: male, 13/50 controls vs 12/50 low-dose and 23/50 high-dose (P Toxicology Program concluded that there was no convincing evidence that BP-A was carcinogenic for rats or mice. However, the marginal increases in leukemias in male and female rats, along with increases in the combined incidence of lymphomas and leukemias in male mice, suggest that BP-A may be associated with increased cancers of the hematopoietic system. Increases in interstitial-cell tumors of the testes in rats were also evidence of carcinogenesis, as was the unusual occurrence of mammary gland fibroadenomas in male rats.

  7. The polar domain of the b subunit of Escherichia coli F1F0-ATPase forms an elongated dimer that interacts with the F1 sector.

    Science.gov (United States)

    Dunn, S D

    1992-04-15

    A soluble form of the b subunit of the F0 sector of the F1F0-ATPase of Escherichia coli has been produced, purified, and characterized. In this form of the protein, designated bsol, residues 25-146 (the carboxyl terminus) of b have been fused to an amino-terminal octapeptide extension derived from the vector pUC8. The inferred subunit molecular weight of bsol is 15,459. bsol protein was expressed in E. coli as a soluble cytoplasmic protein and was readily purified to homogeneity by conventional methods. The molecular weight of bsol, determined by sedimentation equilibrium, was 31,200, indicating that the protein is dimeric. Chemical cross-linking studies supported this conclusion. However, bsol sedimented with a coefficient of just 1.8 S and behaved on size exclusion chromatography with an apparent molecular weight of 80,000-85,000. These results indicate that the protein exists in solution as a highly elongated dimer. The circular dichroism spectrum indicated that bsol is highly alpha-helical. Binding of bsol to F1-ATPase was directly demonstrated by size exclusion chromatography. bsol also inhibited the binding of F1-ATPase to F1-depleted membrane vesicles, as measured by reconstitution of energy-dependent quinacrine fluorescence quenching. This result implies that bsol and F0 compete for binding to the same site on F1. The apparently normal interaction of bsol with F1-ATPase strongly suggests that the recombinant protein assumes the correct structure. No substantial effects of bsol on the ATPase activity of purified F1 were observed.

  8. All eggs are not equal: the maternal environment affects progeny reproduction and developmental fate in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Simon C Harvey

    Full Text Available BACKGROUND: Maternal effects on progeny traits are common and these can profoundly alter progeny life history. Maternal effects can be adaptive, representing attempts to appropriately match offspring phenotype to the expected environment and are often mediated via trade-offs between progeny number and quality. Here we have investigated the effect of maternal food availability on progeny life history in the free-living nematode Caenorhabditis elegans. METHODOLOGY/PRINCIPAL FINDINGS: The maternal environment affects both reproductive traits and progeny development. Comparisons of the progeny of worms from high and low maternal food environments indicates that low maternal food availability reduces progeny reproduction in good environments, increases progeny reproduction in poor environments and decreases the likelihood that progeny will develop as dauer larvae. These analyses also indicate that the effects on progeny are not a simple consequence of changes in maternal body size, but are associated with an increase in the size of eggs produced by worms at low maternal food availabilities. CONCLUSIONS/SIGNIFICANCE: These results indicate that the maternal environment affects both progeny reproduction and development in C. elegans and therefore that all progeny are not equal. The observed effects are consistent with changes to egg provisioning, which are beneficial in harsh environments, and of changes to progeny development, which are beneficial in harsh environments and detrimental in benign environments. These changes in progeny life history suggest that mothers in poor quality environments may be producing larger eggs that are better suited to poor conditions.

  9. 棉酚降解菌株F-1的安全性检验及产芽孢条件优化%Safety testing and condition optimization of producing spore of gossypol degradation strain F-1

    Institute of Scientific and Technical Information of China (English)

    许净净; 李佳; 李卓伟; 朱宝成; 袁洪水

    2013-01-01

      以实验室分离的F-1菌株为出发菌株,将F-1菌株的发酵液进行了小白鼠的毒性试验。试验组小鼠生长发育良好,各个脏器的色泽与大小与对照组小鼠相比无明显差别,证明此菌株为安全菌株。为了将此菌株制备成棉籽饼脱毒菌剂应用于实际生产,对F-1菌株的产芽孢条件进行了系统的研究,采用单因素试验和正交试验的方法确定了F-1的最佳培养基组成为:蔗糖0.5%、牛肉浸膏5%、FeCl30.1%、MgSO4·7H2O 0.05%、NaH2PO4·H2O 0.03%、K2HPO40.05%、MnSO4·H2O 0.02%。在此基础上的最佳培养条件为:pH值7.2、装液量50 ml/250 ml、发酵时间48 h、接种量4%,经优化后芽孢产率达到96.5%。%As a mold mushroom, the toxicity test of strain F-1 was carried out. The mice that fed strain F-1 were all in good health. The results show that compared with the control, the vis⁃cera of the mice that fed strain F-1 had no obvious difference. So it can be proved that the F-1 is a safe strain. To prepare the detoxification agents of cottonseed cake and application in the actual production, the fermentation conditions were optimized. The optimum fermentation medi⁃um and optimum fermentation conditions for the produce spores of F-1 were optimized by using the single factor test and orthogonal test. The results showed that the optimum fermentation me⁃dium was as following: sucrose 0.5%, beef extract 5%, FeCl3 0.1%, MgSO4 · 7H2O 0.05%, NaH2PO4·H2O 0.03%, K2HPO4 0.05%, MnSO4·H2O 0.02%. For the optimum fermentation condi⁃tions, pH value was 7.2, volume was 50 ml/250 ml, fermentation time was 48 h and inoculation amount was 4%. After optimization, the rate of producing spore was up to 96.5%.

  10. Linkage of E2F1 transcriptional network and cell proliferation with respiratory chain activity in breast cancer cells.

    Science.gov (United States)

    Mori, Kazunori; Uchida, Tetsu; Fukumura, Motonori; Tamiya, Shigetoshi; Higurashi, Masato; Sakai, Hirosato; Ishikawa, Fumihiro; Shibanuma, Motoko

    2016-07-01

    Mitochondria are multifunctional organelles; they have been implicated in various aspects of tumorigenesis. In this study, we investigated a novel role of the basal electron transport chain (ETC) activity in cell proliferation by inhibiting mitochondrial replication and transcription (mtR/T) using pharmacological and genetic interventions, which depleted mitochondrial DNA/RNA, thereby inducing ETC deficiency. Interestingly, mtR/T inhibition did not decrease ATP levels despite deficiency in ETC activity in different cell types, including MDA-MB-231 breast cancer cells, but it severely impeded cell cycle progression, specifically progression during G2 and/or M phases in the cancer cells. Under these conditions, the expression of a group of cell cycle regulators was downregulated without affecting the growth signaling pathway. Further analysis suggested that the transcriptional network organized by E2F1 was significantly affected because of the downregulation of E2F1 in response to ETC deficiency, which eventually resulted in the suppression of cell proliferation. Thus, in this study, the E2F1-mediated ETC-dependent mechanism has emerged as the regulatory mechanism of cell cycle progression. In addition to E2F1, FOXM1 and BMYB were also downregulated, which contributed specifically to the defects in G2 and/or M phase progression. Thus, ETC-deficient cancer cells lost their growing ability, including their tumorigenic potential in vivo. ETC deficiency abolished the production of reactive oxygen species (ROS) from the mitochondria and a mitochondria-targeted antioxidant mimicked the deficiency, thereby suggesting that ETC activity signaled through ROS production. In conclusion, this novel coupling between ETC activity and cell cycle progression may be an important mechanism for coordinating cell proliferation and metabolism.

  11. Structure of a thermophilic F1-ATPase inhibited by an ε-subunit: deeper insight into the ε-inhibition mechanism.

    Science.gov (United States)

    Shirakihara, Yasuo; Shiratori, Aya; Tanikawa, Hiromi; Nakasako, Masayoshi; Yoshida, Masasuke; Suzuki, Toshiharu

    2015-08-01

    F1-ATPase (F1) is the catalytic sector in F(o)F1-ATP synthase that is responsible for ATP production in living cells. In catalysis, its three catalytic β-subunits undergo nucleotide occupancy-dependent and concerted open-close conformational changes that are accompanied by rotation of the γ-subunit. Bacterial and chloroplast F1 are inhibited by their own ε-subunit. In the ε-inhibited Escherichia coli F1 structure, the ε-subunit stabilizes the overall conformation (half-closed, closed, open) of the β-subunits by inserting its C-terminal helix into the α3β3 cavity. The structure of ε-inhibited thermophilic F1 is similar to that of E. coli F1, showing a similar conformation of the ε-subunit, but the thermophilic ε-subunit stabilizes another unique overall conformation (open, closed, open) of the β-subunits. The ε-C-terminal helix 2 and hook are conserved between the two structures in interactions with target residues and in their positions. Rest of the ε-C-terminal domains are in quite different conformations and positions, and have different modes of interaction with targets. This region is thought to serve ε-inhibition differently. For inhibition, the ε-subunit contacts the second catches of some of the β- and α-subunits, the N- and C-terminal helices, and some of the Rossmann fold segments. Those contacts, as a whole, lead to positioning of those β- and α- second catches in ε-inhibition-specific positions, and prevent rotation of the γ-subunit. Some of the structural features are observed even in IF1 inhibition in mitochondrial F1.

  12. Maternal-engineered nanomaterial exposure disrupts progeny cardiac function and bioenergetics.

    Science.gov (United States)

    Hathaway, Quincy A; Nichols, Cody E; Shepherd, Danielle L; Stapleton, Phoebe A; McLaughlin, Sarah L; Stricker, Janelle C; Rellick, Stephanie L; Pinti, Mark V; Abukabda, Alaeddin B; McBride, Carroll R; Yi, Jinghai; Stine, Seth M; Nurkiewicz, Timothy R; Hollander, John M

    2017-03-01

    Nanomaterial production is expanding as new industrial and consumer applications are introduced. Nevertheless, the impacts of exposure to these compounds are not fully realized. The present study was designed to determine whether gestational nano-sized titanium dioxide exposure impacts cardiac and metabolic function of developing progeny. Pregnant Sprague-Dawley rats were exposed to nano-aerosols (~10 mg/m(3), 130- to 150-nm count median aerodynamic diameter) for 7-8 nonconsecutive days, beginning at gestational day 5-6 Physiological and bioenergetic effects on heart function and cardiomyocytes across three time points, fetal (gestational day 20), neonatal (4-10 days), and young adult (6-12 wk), were evaluated. Functional analysis utilizing echocardiography, speckle-tracking based strain, and cardiomyocyte contractility, coupled with mitochondrial energetics, revealed effects of nano-exposure. Maternal exposed progeny demonstrated a decrease in E- and A-wave velocities, with a 15% higher E-to-A ratio than controls. Myocytes isolated from exposed animals exhibited ~30% decrease in total contractility, departure velocity, and area of contraction. Bioenergetic analysis revealed a significant increase in proton leak across all ages, accompanied by decreases in metabolic function, including basal respiration, maximal respiration, and spare capacity. Finally, electron transport chain complex I and IV activities were negatively impacted in the exposed group, which may be linked to a metabolic shift. Molecular data suggest that an increase in fatty acid metabolism, uncoupling, and cellular stress proteins may be associated with functional deficits of the heart. In conclusion, gestational nano-exposure significantly impairs the functional capabilities of the heart through cardiomyocyte impairment, which is associated with mitochondrial dysfunction.NEW & NOTEWORTHY Cardiac function is evaluated, for the first time, in progeny following maternal nanomaterial inhalation. The

  13. Transgenerational effects of neonatal hypoxia-ischemia in progeny.

    Science.gov (United States)

    Infante, Smitha K; Rea, Harriett C; Perez-Polo, J R

    2013-10-01

    Neonatal hypoxia-ischemia (HI) affects 60% of low birth weight infants and up to 40% of preterm births. Cell death and brain injury after HI have been shown to cause long-lasting behavioral deficits. By using a battery of behavioral tests on second generation 3-week-old rodents, we found that neonatal HI is associated with behavioral outcomes in the progeny of HI-affected parents. Our results suggest an epigenetic transfer mechanism of some of the neurological symptoms associated with neonatal HI. Elucidating the transfer of brain injury to the next generation after HI calls attention to the risks associated with HI injury and the need for proper treatment to reverse these effects. Assessing the devastating extent of HI's reach serves as a cautionary tale to the risks associated with neonatal HI, and provides an incentive to create improved therapeutic measures to treat HI.

  14. First detection of radon progeny recoil tracks by MIMAC

    Science.gov (United States)

    Riffard, Q.; Santos, D.; Guillaudin, O.; Bosson, G.; Bourrion, O.; Bouvier, J.; Descombes, T.; Fourel, C.; Muraz, J.-F.; Lebreton, L.; Maire, D.; Colas, P.; Ferrer-Ribas, E.; Giomataris, I.; Busto, J.; Fouchez, D.; Brunner, J.; Tao, C.

    2017-06-01

    The MIMAC experiment is a μ-TPC project for directional dark matter search. Directional detection strategy is based on the measurement of the WIMP flux anisotropy due to the solar system motion with respect to the dark matter halo. The main purpose of MIMAC project is the measurement of nuclear recoil energy and 3D direction from the WIMP elastic scattering on target nuclei. Since June 2012 a bi-chamber prototype is operating at the Modane underground laboratory. In this paper, we report the first ionization energy and 3D track observations of NRs produced by the radon progeny. This measurement shows the capability of the MIMAC detector and opens the possibility to explore the low energy recoil directionality signature.

  15. First detection of tracks of radon progeny recoils by MIMAC

    CERN Document Server

    Riffard, Q; Bosson, G; Bourrion, O; Descombes, T; Fourel, C; Guillaudin, O; Muraz, J -F; Colas, P; Ferrer-Ribas, E; Giomataris, I; Busto, J; Fouchez, D; Tao, C; Lebreton, L; Maire, D

    2015-01-01

    The MIMAC experiment is a $\\mu$-TPC matrix project for directional dark matter search. Directional detection is a strategy based on the measurement of the WIMP flux anisotropy due to the solar system motion with respect to the dark matter halo. The main purpose of MIMAC project is the measurement of the energy and the direction of nuclear recoils in 3D produced by elastic scattering of WIMPs. Since June 2012 a bi-chamber prototype is operating at the Modane underground laboratory. In this paper, we report the first ionization energy and 3D track observations of nuclear recoils produced by the radon progeny. This measurement shows the capability of the MIMAC detector and opens the possibility to explore the low energy recoil directionality signature.

  16. Thoron progeny size distribution in monazite storage facility.

    Science.gov (United States)

    Rogozina, Marina; Zhukovsky, Michael; Ekidin, Aleksey; Vasyanovich, Maksim

    2014-11-01

    Field experiments in the atmosphere of monazite warehouses with a high content of (220)Rn progeny concentration were conducted. Size distribution of aerosol particles was measured with the combined use of diffusion battery with varied capture elements and cascade impactor. Four (212)Pb aerosol modes were detected-three in the ultrafine region (aerosol median thermodynamic diameters ∼0.3, 1 and 5 nm) and one with an aerosol median aerodynamic diameter of 500 nm. The activity fraction of aerosol particles with the size <10 nm is nearly 20-25 %. The dose conversion factor for EEC₂₂₀Rn exposure, obtained on the basis of the aerosol size distribution and existing research data on lung absorption types of (212)Pb aerosols, is close to 180 nSv per Bq h m(-3).

  17. Dedifferentiated adipocyte-derived progeny cells (DFAT cells)

    Science.gov (United States)

    Wei, Shengjuan; Zan, Linsen; Hausman, Gary J; Rasmussen, Theodore P; Bergen, Werner G; Dodson, Michael V

    2013-01-01

    Analyses of mature adipocytes have shown that they possess a reprogramming ability in vitro, which is associated with dedifferentiation. The subsequent dedifferentiated fat cells (DFAT cells) are multipotent and can differentiate into adipocytes and other cell types as well. Mature adipocytes can be easily obtained by biopsy, and the cloned progeny cells are homogeneous in vitro. Therefore, DFAT cells (a new type of stem cell) may provide an excellent source of cells for tissue regeneration, engineering and disease treatment. The dedifferentiation of mature adipocytes, the multipotent capacity of DFAT cells and comparisons and contrasts with mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPS) are discussed in this review. PMID:23991357

  18. Radon progeny deposition in track-detection diffusion chambers

    CERN Document Server

    Pressyanov, D; Simeonov, G

    1999-01-01

    The sensitivity dependence for cylindrical diffusion chambers that are used for radon track-detection measurements on the deposition fraction of radon progeny atoms has been theoretically studied (sensitivity is the ratio: area track density/integrated sup 2 sup 2 sup 2 Rn activity concentration). Experimentally, the sensitivity values of both the metal- and plastic-made chambers were determined. Results indicate that the experimental sensitivity for metal chambers is in accordance with the theoretical model while a deviation of 15% is observed for plastic chambers. The uncertainty in the sensitivity values that is related to possible variations of the diffusion coefficient for sup 2 sup 1 sup 8 Po atoms was estimated to be less than 10%. (author)

  19. Characterization of an F1 Deletion Mutant of Yersinia pestis CO92, Pathogenic Role of F1 Antigen in Bubonic and Pneumonic Plague, and Evaluation of Sensitivity and Specificity of F1 Antigen Capture-Based Dipsticks▿

    Science.gov (United States)

    Sha, Jian; Endsley, Janice J.; Kirtley, Michelle L.; Foltz, Sheri M.; Huante, Matthew B.; Erova, Tatiana E.; Kozlova, Elena V.; Popov, Vsevolod L.; Yeager, Linsey A.; Zudina, Irina V.; Motin, Vladimir L.; Peterson, Johnny W.; DeBord, Kristin L.; Chopra, Ashok K.

    2011-01-01

    We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipsticks, an in-frame F1-deficient mutant of CO92 (Δcaf) was generated by homologous recombination and used as a negative control. Based on genetic, antigenic/immunologic, and electron microscopic analyses, the Δcaf mutant was devoid of a capsule. The growth rate of the Δcaf mutant generally was similar to that of the wild-type (WT) bacterium at both 26 and 37°C, although the mutant's growth dropped slightly during the late phase at 37°C. The Δcaf mutant was as virulent as WT CO92 in the pneumonic plague mouse model; however, it was attenuated in developing bubonic plague. Both dipsticks had similar sensitivities, requiring a minimum of 0.5 μg/ml of purified F1 antigen or 1 × 105 to 5 × 105 CFU/ml of WT CO92 for positive results, while the blood samples were negative for up to 1 × 108 CFU/ml of the Δcaf mutant. Our studies demonstrated the diagnostic potential of two plague dipsticks in detecting capsular-positive strains of Y. pestis in bubonic and pneumonic plague. PMID:21367990

  20. 26 CFR 5c.44F-1 - Leases and qualified research expenses.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 14 2010-04-01 2010-04-01 false Leases and qualified research expenses. 5c.44F-1 Section 5c.44F-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) TEMPORARY INCOME TAX REGULATIONS UNDER THE ECONOMIC RECOVERY TAX ACT OF 1981 § 5c...

  1. Catalytic properties of Escherichia coli F1-ATPase depleted of endogenous nucleotides.

    Science.gov (United States)

    Senior, A E; Lee, R S; al-Shawi, M K; Weber, J

    1992-09-01

    Nucleotide-depleted Escherichia coli F1 was prepared by the procedure of Wise et al. (1983, Biochem. J. 215, 343-350). This enzyme had high rates of steady-state ATPase and GTPase activity. When "unisite" ATP hydrolysis was measured using an F1/ATP concentration ratio of 10, all of the substoichiometric ATP became bound to the high-affinity catalytic site and none became bound to noncatalytic sites. The association rate constant for ATP binding was 7 x 10(5) M-1 s-1 and the KdATP was 7.9 x 10(-10) M, as compared to values of 3.8 x 10(5) M-1 s-1 and 1.9 x 10(-10) M, respectively, in native (i.e., nucleotide-replete) F1. Rate constants for bound ATP hydrolysis, ATP resynthesis, and P(i) release, and the reaction equilibrium constant, were similar in nucleotide-depleted and native F1. Therefore, we conclude that occupancy of the noncatalytic sites is not required for formation of the high-affinity catalytic site of F1 and has no significant effect on unisite catalysis. In further experiments we looked for the occurrence of inhibitory, catalytic-site-bound MgADP in E. coli F1. Such an entity has been reported for chloroplast and mitochondrial F1. However, our experiments gave no indication for inhibitory MgADP in E. coli F1.

  2. E2F-1-Induced p53-independent apoptosis in transgenic mice

    DEFF Research Database (Denmark)

    Holmberg, Christian Henrik; Helin, K.; Sehested, M.;

    1998-01-01

    involving increased apoptosis in the germinal epithelium. This effect was potentiated by simultaneous overexpression of DP-1. Testicular atrophy as a result of overexpression of E2F-1 and DP-1 is independent of functional p53, since p53-nullizygous transgenic mice overexpressing E2F-1 and DP-1 also suffered...

  3. 26 CFR 1.665(f)-1A - Undistributed capital gain.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Undistributed capital gain. 1.665(f)-1A Section... Beginning on Or After January 1, 1969 § 1.665(f)-1A Undistributed capital gain. (a) Domestic trusts. (1) The term undistributed capital gain means (in the case of a trust other than a foreign trust created by a...

  4. 26 CFR 1.514(f)-1 - Definition of business lease.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Definition of business lease. 1.514(f)-1 Section... § 1.514(f)-1 Definition of business lease. (a) In general. The term business lease means any lease...) if at the close of the organization's taxable year there is a business lease indebtedness as...

  5. 77 FR 20038 - Employment Authorization for Syrian F-1 Nonimmigrant Students Experiencing Severe Economic...

    Science.gov (United States)

    2012-04-03

    ... minimum course load requirement, unless the student's course of study is in a language study program. See... maintains his or her TPS, then the student maintains F-1 status and TPS concurrently. Under the second... SECURITY RIN 1653-ZA04 Employment Authorization for Syrian F-1 Nonimmigrant Students Experiencing...

  6. 26 CFR 301.6501(f)-1 - Personal holding company tax.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Personal holding company tax. 301.6501(f)-1... Collection § 301.6501(f)-1 Personal holding company tax. If a corporation which is a personal holding company... time during the last half of such taxable year, more than 50 percent in value of the...

  7. Chromosomal Constitution and Karyotype of Intergeneric F1 Hybrids Between Saccharum spp.and Erianthus fulvus Based on GISH%基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析

    Institute of Scientific and Technical Information of China (English)

    王先宏; 杨清辉; 李富生; 何丽莲; 何顺长

    2011-01-01

    [Objective] The objective of current experiment was to study the chromosomal constitution and karyotype of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and Erianthusfulvus. [Method] Both monocolor and bicolor GISH (Genomic in situ hybridization) were performed using digoxigenin-labeled E. fulvus DNA and biotin-labeled female parental DNA as probes, the karyotypes of 3 hybrids were analyzed based on the result of GISHs. [ Result ] The results of GISHs revealed the transmission of 7 to 10 E. fulvus chromosomes into progenies. The chromosomes of E. fulvus were broken because of the female parents prevented alien genetic materials from introgressing during distant hybridization and resulted in elimination and fragmentation of E. fulvus chromosomes. The GISH result on nuclear of progeny showed the chromatins came from male and female parent are not intermixed and stays at different regions. The karyotype formulas of 3 hybrids were 2n=92=80m (4SAT)+12sm.2n=88=82m+6sm and 2n=88=78m(2SAT)+10sm, and their karyotypes were 2C, 2C and 2B, respectively. [Conclusion]Chromosomal constitution of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and E. fulvus were n+n and 2n+n. The results of karpotype analysis showed that the chromosomes of these three progenies are primitive.%[目的]探讨甘蔗(Saccharum spp.)与蔗茅(Erianthus fulvus)杂交F1材料的染色体组成及核型差异.[方法]以甘蔗与蔗茅间杂交所获得的16份F1材料为研究对象进行单色及双色基因组原位杂交(genomic in situ hybridization,GISH)研究,在GISH的基础上对3份材料的中期染色体进行核型分析.[结果]GISH的研究结果表明,子代材料中具有7-10条不等的父本染色体; 由于母本遗传物质对父本遗传物质的排斥作用引起的染色体消减及片段化导致父本染色体在于代中有断裂的现象;研究结果表明,在间期核中亲本染色质

  8. Arsenate reduction and methylation in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 investigated with X-ray absorption near edge structure

    Energy Technology Data Exchange (ETDEWEB)

    Su, S.M., E-mail: shimingsu@163.com [Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing (China); Zeng, X.B., E-mail: zengxb@ieda.org.cn [Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing (China); Li, L.F.; Duan, R.; Bai, L.Y. [Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing (China); Li, A.G.; Wang, J.; Jiang, S. [Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai (China)

    2012-12-15

    Highlights: Black-Right-Pointing-Pointer Three fungal strains are capable of As(V) reduction and methylation. Black-Right-Pointing-Pointer As(V) reduction might be more easily processed than the methylation in fungal cells. Black-Right-Pointing-Pointer As sequestration and speciation transformation might be the detoxification processes. - Abstract: Synchrotron radiation-based X-ray absorption near edge structure (XANES) was introduced to directly analysis chemical species of arsenic (As) in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 capable of As accumulation and volatilisation. After exposure to As(V) of 500 mg L{sup -1} for 15 days, a total of 60.5% and 65.3% of the accumulated As in the cells of T. asperellum SM-12F1 and P. janthinellum SM-12F4, respectively, was As(III), followed by 31.3% and 32.4% DMA (dimethylarsinic acid), 8.3% and 2.3% MMA (monomethylarsonic acid), respectively. However, for F. oxysporum CZ-8F1, 54.5% of the accumulated As was As(III), followed by 37.8% MMA and 7.7% As(V). The reduction and methylation of As(V) formed As(III), MMA, and DMA as the primacy products, and the reduction of As(V) might be more easily processed than the methylation. These results will help to understanding the mechanisms of As detoxification and its future application in bioremediation.

  9. Observation of $\\bar{B}^0_{(s)}\\rightarrow J/\\psi f_1(1285)$ decays and measurement of the $f_1(1285)$ mixing angle

    CERN Document Server

    Aaij, R; Adinolfi, M; Adrover, C; Affolder, A; Ajaltouni, Z; Albrecht, J; Alessio, F; Alexander, M; Ali, S; Alkhazov, G; Alvarez Cartelle, P; Alves Jr, A A; Amato, S; Amerio, S; Amhis, Y; Anderlini, L; Anderson, J; Andreassen, R; Andreotti, M; Andrews, J E; Appleby, R B; Aquines Gutierrez, O; Archilli, F; Artamonov, A; Artuso, M; Aslanides, E; Auriemma, G; Baalouch, M; Bachmann, S; Back, J J; Badalov, A; Baesso, C; Balagura, V; Baldini, W; Barlow, R J; Barschel, C; Barsuk, S; Barter, W; Batozskaya, V; Bauer, Th; Bay, A; Beddow, J; Bedeschi, F; Bediaga, I; Belogurov, S; Belous, K; Belyaev, I; Ben-Haim, E; Bencivenni, G; Benson, S; Benton, J; Berezhnoy, A; Bernet, R; Bettler, M -O; van Beuzekom, M; Bien, A; Bifani, S; Bird, T; Bizzeti, A; Bjørnstad, P M; Blake, T; Blanc, F; Blouw, J; Blusk, S; Bocci, V; Bondar, A; Bondar, N; Bonivento, W; Borghi, S; Borgia, A; Bowcock, T J V; Bowen, E; Bozzi, C; Brambach, T; van den Brand, J; Bressieux, J; Brett, D; Britsch, M; Britton, T; Brook, N H; Brown, H; Bursche, A; Busetto, G; Buytaert, J; Cadeddu, S; Calabrese, R; Callot, O; Calvi, M; Calvo Gomez, M; Camboni, A; Campana, P; Campora Perez, D; Carbone, A; Carboni, G; Cardinale, R; Cardini, A; Carranza-Mejia, H; Carson, L; Carvalho Akiba, K; Casse, G; Castillo Garcia, L; Cattaneo, M; Cauet, Ch; Cenci, R; Charles, M; Charpentier, Ph; Cheung, S -F; Chiapolini, N; Chrzaszcz, M; Ciba, K; Cid Vidal, X; Ciezarek, G; Clarke, P E L; Clemencic, M; Cliff, H V; Closier, J; Coca, C; Coco, V; Cogan, J; Cogneras, E; Collins, P; Comerma-Montells, A; Contu, A; Cook, A; Coombes, M; Coquereau, S; Corti, G; Couturier, B; Cowan, G A; Craik, D C; Cruz Torres, M; Cunliffe, S; Currie, R; D'Ambrosio, C; David, P; David, P N Y; Davis, A; De Bonis, I; De Bruyn, K; De Capua, S; De Cian, M; De Miranda, J M; De Paula, L; De Silva, W; De Simone, P; Decamp, D; Deckenhoff, M; Del Buono, L; Déléage, N; Derkach, D; Deschamps, O; Dettori, F; Di Canto, A; Dijkstra, H; Dogaru, M; Donleavy, S; Dordei, F; Dosil Suárez, A; Dossett, D; Dovbnya, A; Dupertuis, F; Durante, P; Dzhelyadin, R; Dziurda, A; Dzyuba, A; Easo, S; Egede, U; Egorychev, V; Eidelman, S; van Eijk, D; Eisenhardt, S; Eitschberger, U; Ekelhof, R; Eklund, L; El Rifai, I; Elsasser, Ch; Falabella, A; Färber, C; Farinelli, C; Farry, S; Ferguson, D; Fernandez Albor, V; Ferreira Rodrigues, F; Ferro-Luzzi, M; Filippov, S; Fiore, M; Fiorini, M; Fitzpatrick, C; Fontana, M; Fontanelli, F; Forty, R; Francisco, O; Frank, M; Frei, C; Frosini, M; Furfaro, E; Gallas Torreira, A; Galli, D; Gandelman, M; Gandini, P; Gao, Y; Garofoli, J; Garosi, P; Garra Tico, J; Garrido, L; Gaspar, C; Gauld, R; Gersabeck, E; Gersabeck, M; Gershon, T; Ghez, Ph; Gibson, V; Giubega, L; Gligorov, V V; Göbel, C; Golubkov, D; Golutvin, A; Gomes, A; Gorbounov, P; Gordon, H; Grabalosa Gándara, M; Graciani Diaz, R; Granado Cardoso, L A; Graugés, E; Graziani, G; Grecu, A; Greening, E; Gregson, S; Griffith, P; Grillo, L; Grünberg, O; Gui, B; Gushchin, E; Guz, Yu; Gys, T; Hadjivasiliou, C; Haefeli, G; Haen, C; Hafkenscheid, T W; Haines, S C; Hall, S; Hamilton, B; Hampson, T; Hansmann-Menzemer, S; Harnew, N; Harnew, S T; Harrison, J; Hartmann, T; He, J; Head, T; Heijne, V; Hennessy, K; Henrard, P; Hernando Morata, J A; van Herwijnen, E; Heß, M; Hicheur, A; Hicks, E; Hill, D; Hoballah, M; Hombach, C; Hulsbergen, W; Hunt, P; Huse, T; Hussain, N; Hutchcroft, D; Hynds, D; Iakovenko, V; Idzik, M; Ilten, P; Jacobsson, R; Jaeger, A; Jans, E; Jaton, P; Jawahery, A; Jing, F; John, M; Johnson, D; Jones, C R; Joram, C; Jost, B; Kaballo, M; Kandybei, S; Kanso, W; Karacson, M; Karbach, T M; Kenyon, I R; Ketel, T; Khanji, B; Kochebina, O; Komarov, I; Koopman, R F; Koppenburg, P; Korolev, M; Kozlinskiy, A; Kravchuk, L; Kreplin, K; Kreps, M; Krocker, G; Krokovny, P; Kruse, F; Kucharczyk, M; Kudryavtsev, V; Kurek, K; Kvaratskheliya, T; La Thi, V N; Lacarrere, D; Lafferty, G; Lai, A; Lambert, D; Lambert, R W; Lanciotti, E; Lanfranchi, G; Langenbruch, C; Latham, T; Lazzeroni, C; Le Gac, R; van Leerdam, J; Lees, J -P; Lefèvre, R; Leflat, A; Lefrançois, J; Leo, S; Leroy, O; Lesiak, T; Leverington, B; Li, Y; Li Gioi, L; Liles, M; Lindner, R; Linn, C; Liu, B; Liu, G; Lohn, S; Longstaff, I; Lopes, J H; Lopez-March, N; Lu, H; Lucchesi, D; Luisier, J; Luo, H; Luppi, E; Lupton, O; Machefert, F; Machikhiliyan, I V; Maciuc, F; Maev, O; Malde, S; Manca, G; Mancinelli, G; Maratas, J; Marconi, U; Marino, P; Märki, R; Marks, J; Martellotti, G; Martens, A; Martín Sánchez, A; Martinelli, M; Martinez Santos, D; Martins Tostes, D; Martynov, A; Massafferri, A; Matev, R; Mathe, Z; Matteuzzi, C; Maurice, E; Mazurov, A; McCann, M; McCarthy, J; McNab, A; McNulty, R; McSkelly, B; Meadows, B; Meier, F; Meissner, M; Merk, M; Milanes, D A; Minard, M -N; Molina Rodriguez, J; Monteil, S; Moran, D; Morawski, P; Mordà, A; Morello, M J; Mountain, R; Mous, I; Muheim, F; Müller, K; Muresan, R; Muryn, B; Muster, B; Naik, P; Nakada, T; Nandakumar, R; Nasteva, I; Needham, M; Neubert, S; Neufeld, N; Nguyen, A D; Nguyen, T D; Nguyen-Mau, C; Nicol, M; Niess, V; Niet, R; Nikitin, N; Nikodem, T; Nomerotski, A; Novoselov, A; Oblakowska-Mucha, A; Obraztsov, V; Oggero, S; Ogilvy, S; Okhrimenko, O; Oldeman, R; Onderwater, G; Orlandea, M; Otalora Goicochea, J M; Owen, P; Oyanguren, A; Pal, B K; Palano, A; Palutan, M; Panman, J; Papanestis, A; Pappagallo, M; Parkes, C; Parkinson, C J; Passaleva, G; Patel, G D; Patel, M; Patrick, G N; Patrignani, C; Pavel-Nicorescu, C; Pazos Alvarez, A; Pearce, A; Pellegrino, A; Penso, G; Pepe Altarelli, M; Perazzini, S; Perez Trigo, E; Pérez-Calero Yzquierdo, A; Perret, P; Perrin-Terrin, M; Pescatore, L; Pesen, E; Pessina, G; Petridis, K; Petrolini, A; Phan, A; Picatoste Olloqui, E; Pietrzyk, B; Pilař, T; Pinci, D; Playfer, S; Plo Casasus, M; Polci, F; Polok, G; Poluektov, A; Polycarpo, E; Popov, A; Popov, D; Popovici, B; Potterat, C; Powell, A; Prisciandaro, J; Pritchard, A; Prouve, C; Pugatch, V; Puig Navarro, A; Punzi, G; Qian, W; Rachwal, B; Rademacker, J H; Rakotomiaramanana, B; Rangel, M S; Raniuk, I; Rauschmayr, N; Raven, G; Redford, S; Reichert, S; Reid, M M; dos Reis, A C; Ricciardi, S; Richards, A; Rinnert, K; Rives Molina, V; Roa Romero, D A; Robbe, P; Roberts, D A; Rodrigues, A B; Rodrigues, E; Rodriguez Perez, P; Roiser, S; Romanovsky, V; Romero Vidal, A; Rotondo, M; Rouvinet, J; Ruf, T; Ruffini, F; Ruiz, H; Ruiz Valls, P; Sabatino, G; Saborido Silva, J J; Sagidova, N; Sail, P; Saitta, B; Salustino Guimaraes, V; Sanmartin Sedes, B; Santacesaria, R; Santamarina Rios, C; Santovetti, E; Sapunov, M; Sarti, A; Satriano, C; Satta, A; Savrie, M; Savrina, D; Schiller, M; Schindler, H; Schlupp, M; Schmelling, M; Schmidt, B; Schneider, O; Schopper, A; Schune, M -H; Schwemmer, R; Sciascia, B; Sciubba, A; Seco, M; Semennikov, A; Senderowska, K; Sepp, I; Serra, N; Serrano, J; Seyfert, P; Shapkin, M; Shapoval, I; Shcheglov, Y; Shears, T; Shekhtman, L; Shevchenko, O; Shevchenko, V; Shires, A; Silva Coutinho, R; Sirendi, M; Skidmore, N; Skwarnicki, T; Smith, N A; Smith, E; Smith, E; Smith, J; Smith, M; Sokoloff, M D; Soler, F J P; Soomro, F; Souza, D; Souza De Paula, B; Spaan, B; Sparkes, A; Spradlin, P; Stagni, F; Stahl, S; Steinkamp, O; Stevenson, S; Stoica, S; Stone, S; Storaci, B; Straticiuc, M; Straumann, U; Subbiah, V K; Sun, L; Sutcliffe, W; Swientek, S; Syropoulos, V; Szczekowski, M; Szczypka, P; Szilard, D; Szumlak, T; T'Jampens, S; Teklishyn, M; Tellarini, G; Teodorescu, E; Teubert, F; Thomas, C; Thomas, E; van Tilburg, J; Tisserand, V; Tobin, M; Tolk, S; Tomassetti, L; Tonelli, D; Topp-Joergensen, S; Torr, N; Tournefier, E; Tourneur, S; Tran, M T; Tresch, M; Tsaregorodtsev, A; Tsopelas, P; Tuning, N; Ubeda Garcia, M; Ukleja, A; Ustyuzhanin, A; Uwer, U; Vagnoni, V; Valenti, G; Vallier, A; Vazquez Gomez, R; Vazquez Regueiro, P; Vázquez Sierra, C; Vecchi, S; Velthuis, J J; Veltri, M; Veneziano, G; Vesterinen, M; Viaud, B; Vieira, D; Vilasis-Cardona, X; Vollhardt, A; Volyanskyy, D; Voong, D; Vorobyev, A; Vorobyev, V; Voß, C; Voss, H; Waldi, R; Wallace, C; Wallace, R; Wandernoth, S; Wang, J; Ward, D R; Watson, N K; Webber, A D; Websdale, D; Whitehead, M; Wicht, J; Wiechczynski, J; Wiedner, D; Wiggers, L; Wilkinson, G; Williams, M P; Williams, M; Wilson, F F; Wimberley, J; Wishahi, J; Wislicki, W; Witek, M; Wormser, G; Wotton, S A; Wright, S; Wu, S; Wyllie, K; Xie, Y; Xing, Z; Yang, Z; Yuan, X; Yushchenko, O; Zangoli, M; Zavertyaev, M; Zhang, F; Zhang, L; Zhang, W C; Zhang, Y; Zhelezov, A; Zhokhov, A; Zhong, L; Zvyagin, A

    2014-01-01

    Decays of $\\bar{B}^0_(s)$ and $\\bar{B}^0$ mesons into $J/\\psi \\pi^+\\pi^-\\pi^+\\pi^-$ final states, produced in $pp$ collisions at the LHC, are investigated using data corresponding to an integrated luminosity of 3 fb$^{-1}$ collected with the LHCb detector. $\\bar{B}^0_{(s)}\\to J/\\psi f_1(1285)$ decays are seen for the first time, and the branching fractions are measured. Using these rates, the $f_1(1285)$ mixing angle between strange and non-strange components of its wave function in the $q\\overline{q}$ structure model is determined to be $\\pm(24.0^{\\,+3.1\\,+0.6}_{\\,-2.6\\,-0.8})^{\\circ}$. Implications on the possible tetraquark nature of the $f_1(1285)$ are discussed.

  10. Observation of B(s)(0) → J/ψ f1(1285) decays and measurement of the f1(1285) mixing angle.

    Science.gov (United States)

    Aaij, R; Adeva, B; Adinolfi, M; Adrover, C; Affolder, A; Ajaltouni, Z; Albrecht, J; Alessio, F; Alexander, M; Ali, S; Alkhazov, G; Alvarez Cartelle, P; Alves, A A; Amato, S; Amerio, S; Amhis, Y; Anderlini, L; Anderson, J; Andreassen, R; Andreotti, M; Andrews, J E; Appleby, R B; Aquines Gutierrez, O; Archilli, F; Artamonov, A; Artuso, M; Aslanides, E; Auriemma, G; Baalouch, M; Bachmann, S; Back, J J; Badalov, A; Baesso, C; Balagura, V; Baldini, W; Barlow, R J; Barschel, C; Barsuk, S; Barter, W; Batozskaya, V; Bauer, Th; Bay, A; Beddow, J; Bedeschi, F; Bediaga, I; Belogurov, S; Belous, K; Belyaev, I; Ben-Haim, E; Bencivenni, G; Benson, S; Benton, J; Berezhnoy, A; Bernet, R; Bettler, M-O; van Beuzekom, M; Bien, A; Bifani, S; Bird, T; Bizzeti, A; Bjørnstad, P M; Blake, T; Blanc, F; Blouw, J; Blusk, S; Bocci, V; Bondar, A; Bondar, N; Bonivento, W; Borghi, S; Borgia, A; Bowcock, T J V; Bowen, E; Bozzi, C; Brambach, T; van den Brand, J; Bressieux, J; Brett, D; Britsch, M; Britton, T; Brook, N H; Brown, H; Bursche, A; Busetto, G; Buytaert, J; Cadeddu, S; Calabrese, R; Callot, O; Calvi, M; Calvo Gomez, M; Camboni, A; Campana, P; Campora Perez, D; Carbone, A; Carboni, G; Cardinale, R; Cardini, A; Carranza-Mejia, H; Carson, L; Carvalho Akiba, K; Casse, G; Castillo Garcia, L; Cattaneo, M; Cauet, Ch; Cenci, R; Charles, M; Charpentier, Ph; Cheung, S-F; Chiapolini, N; Chrzaszcz, M; Ciba, K; Cid Vidal, X; Ciezarek, G; Clarke, P E L; Clemencic, M; Cliff, H V; Closier, J; Coca, C; Coco, V; Cogan, J; Cogneras, E; Collins, P; Comerma-Montells, A; Contu, A; Cook, A; Coombes, M; Coquereau, S; Corti, G; Couturier, B; Cowan, G A; Craik, D C; Cruz Torres, M; Cunliffe, S; Currie, R; D'Ambrosio, C; David, P; David, P N Y; Davis, A; De Bonis, I; De Bruyn, K; De Capua, S; De Cian, M; De Miranda, J M; De Paula, L; De Silva, W; De Simone, P; Decamp, D; Deckenhoff, M; Del Buono, L; Déléage, N; Derkach, D; Deschamps, O; Dettori, F; Di Canto, A; Dijkstra, H; Dogaru, M; Donleavy, S; Dordei, F; Dosil Suárez, A; Dossett, D; Dovbnya, A; Dupertuis, F; Durante, P; Dzhelyadin, R; Dziurda, A; Dzyuba, A; Easo, S; Egede, U; Egorychev, V; Eidelman, S; van Eijk, D; Eisenhardt, S; Eitschberger, U; Ekelhof, R; Eklund, L; El Rifai, I; Elsasser, Ch; Falabella, A; Färber, C; Farinelli, C; Farry, S; Ferguson, D; Fernandez Albor, V; Ferreira Rodrigues, F; Ferro-Luzzi, M; Filippov, S; Fiore, M; Fiorini, M; Fitzpatrick, C; Fontana, M; Fontanelli, F; Forty, R; Francisco, O; Frank, M; Frei, C; Frosini, M; Furfaro, E; Gallas Torreira, A; Galli, D; Gandelman, M; Gandini, P; Gao, Y; Garofoli, J; Garosi, P; Garra Tico, J; Garrido, L; Gaspar, C; Gauld, R; Gersabeck, E; Gersabeck, M; Gershon, T; Ghez, Ph; Gibson, V; Giubega, L; Gligorov, V V; Göbel, C; Golubkov, D; Golutvin, A; Gomes, A; Gorbounov, P; Gordon, H; Grabalosa Gándara, M; Graciani Diaz, R; Granado Cardoso, L A; Graugés, E; Graziani, G; Grecu, A; Greening, E; Gregson, S; Griffith, P; Grillo, L; Grünberg, O; Gui, B; Gushchin, E; Guz, Yu; Gys, T; Hadjivasiliou, C; Haefeli, G; Haen, C; Hafkenscheid, T W; Haines, S C; Hall, S; Hamilton, B; Hampson, T; Hansmann-Menzemer, S; Harnew, N; Harnew, S T; Harrison, J; Hartmann, T; He, J; Head, T; Heijne, V; Hennessy, K; Henrard, P; Hernando Morata, J A; van Herwijnen, E; Heß, M; Hicheur, A; Hicks, E; Hill, D; Hoballah, M; Hombach, C; Hulsbergen, W; Hunt, P; Huse, T; Hussain, N; Hutchcroft, D; Hynds, D; Iakovenko, V; Idzik, M; Ilten, P; Jacobsson, R; Jaeger, A; Jans, E; Jaton, P; Jawahery, A; Jing, F; John, M; Johnson, D; Jones, C R; Joram, C; Jost, B; Kaballo, M; Kandybei, S; Kanso, W; Karacson, M; Karbach, T M; Kenyon, I R; Ketel, T; Khanji, B; Kochebina, O; Komarov, I; Koopman, R F; Koppenburg, P; Korolev, M; Kozlinskiy, A; Kravchuk, L; Kreplin, K; Kreps, M; Krocker, G; Krokovny, P; Kruse, F; Kucharczyk, M; Kudryavtsev, V; Kurek, K; Kvaratskheliya, T; La Thi, V N; Lacarrere, D; Lafferty, G; Lai, A; Lambert, D; Lambert, R W; Lanciotti, E; Lanfranchi, G; Langenbruch, C; Latham, T; Lazzeroni, C; Le Gac, R; van Leerdam, J; Lees, J-P; Lefèvre, R; Leflat, A; Lefrançois, J; Leo, S; Leroy, O; Lesiak, T; Leverington, B; Li, Y; Li Gioi, L; Liles, M; Lindner, R; Linn, C; Liu, B; Liu, G; Lohn, S; Longstaff, I; Lopes, J H; Lopez-March, N; Lu, H; Lucchesi, D; Luisier, J; Luo, H; Luppi, E; Lupton, O; Machefert, F; Machikhiliyan, I V; Maciuc, F; Maev, O; Malde, S; Manca, G; Mancinelli, G; Maratas, J; Marconi, U; Marino, P; Märki, R; Marks, J; Martellotti, G; Martens, A; Martín Sánchez, A; Martinelli, M; Martinez Santos, D; Martins Tostes, D; Martynov, A; Massafferri, A; Matev, R; Mathe, Z; Matteuzzi, C; Maurice, E; Mazurov, A; McCann, M; McCarthy, J; McNab, A; McNulty, R; McSkelly, B; Meadows, B; Meier, F; Meissner, M; Merk, M; Milanes, D A; Minard, M-N; Molina Rodriguez, J; Monteil, S; Moran, D; Morawski, P; Mordà, A; Morello, M J

    2014-03-01

    Decays of B(s)(0) and B(0) mesons into J/ψ π+π-π+π- final states, produced in pp collisions at the LHC, are investigated using data corresponding to an integrated luminosity of 3 fb-1 collected with the LHCb detector. B(s)(0) → J/ψ f1(1285) decays are seen for the first time, and the branching fractions are measured. Using these rates, the f1(1285) mixing angle between strange and nonstrange components of its wave function in the qq structure model is determined to be ±(24.0-2.6-0.8+3.1+0.6)°. Implications on the possible tetraquark nature of the f1(1285) are discussed.

  11. Relation of Zn, Fe, Cu and Mn between progenies of mate-tree and soil

    Directory of Open Access Journals (Sweden)

    Josiane Cava Guimarães

    2014-06-01

    Full Text Available The mate tea tree (Ilex Paraguariensis St. Hil. has considerable growth in acid a low fertility soils. The knowledge of soil and plant relation will contribute to genetic improvement programs, as highly capable progenies in nutrient acquisition may be selected. The objective of this study was to evaluate the interference of provenance and, or progenies, in relations established among the extractable contents of Zn, Fe, Cu and Mn, via Mehlich-1 (1:10; and EDTA (1%, with the corresponding contents in the mate tea tree leaves. In the experiment two provenances, Ivaí-PR and Barão de Cotegipe-RS, with five progenies each considered as treatments. The samples were distributed in four randomized blocks, totalizing 120 plant leaf samples, related to 120 soil samples of a Red Distrophic Latosol. The results were analyzed and fitted in regression equations. In Ivaí provenance Zn and Mn from the soil correlated with their contents in the leaves for progeny 04, for both extracts. For provenances 08 and 10, soil Mn via EDTA correlated with leaf Mn contents, while via Mehlich-1 only for progeny 10. In the provenance of Barão de Cotegipe, the correlations between soil and leaves for Zn, Fe and Cu occurred for the EDTA extract in the progenies 61, 65 and 69 respectively. For Mn and Cu, via Melich-1 the correlations occurred for progenies 53 and 69 respectively, and still for Cu, via EDTA, for progeny 53.

  12. Prediction of barley progeny performance in the presence of genotype-environment interaction

    NARCIS (Netherlands)

    Schut, J.W.; Dourleijn, C.J.

    2000-01-01

    Twenty recombinant inbred line (RIL) populations of European two-row spring barley and their parents were tested in six environments in the Netherlands to investigate the prediction of progeny yield level, yield variance, stability level and stability variance, based on parent information. Progeny y

  13. Deposition pattern of inhaled radon progeny size distribution in human lung

    Directory of Open Access Journals (Sweden)

    Amer Mohamed

    2014-07-01

    Full Text Available One of the important factors controlling the distribution of radiation dose to the different portions of the human respiratory tract is the deposition pattern of radon progeny containing aerosol. Based on the activity size distribution parameters of radon progeny, which were measured in Minia University, the deposition behavior of radon progeny (attached and unattached has been studied by using a stochastic deposition model. The attached fraction was collected using a low pressure Berner cascade impactor technique. A screen diffusion battery was used for collecting the unattached fraction. Most of the attached activities for 222Rn progeny were associated with aerosol particles of the accumulation mode. The bronchial deposition fraction of particles in the size range of attached radon progeny was found to be lower than those of unattached progeny. The effect of radon progeny deposition by adult male has been also studied for various levels of physical exertion. An increase in the breathing rate was found to decrease the fraction with which inhaled progeny were deposited in the bronchi. As the ventilation rate increases from 0.54 to 1.5 m3 h−1, the average deposition fraction of airway generation 1 through 8 are expected to decrease by 22% for 1.4 nm particles and by 38% for 150 nm particles.

  14. CDK4, pRB and E2F1: connected to insulin

    Directory of Open Access Journals (Sweden)

    Blanchet Emilie

    2010-02-01

    Full Text Available Abstract Pancreatic β-cells are metabolic sensors involved in the control of glucose homeostasis. This particular cell type controls insulin secretion through a fine-tuned process, which dregulation have important pathological consequences, such as observed during type 2 diabetes. We recently implicated E2F1 in the control of glucose homeostasis. First we showed that E2f1-/- mice have decreased pancreatic size, as the result of impaired postnatal pancreatic growth. We observed in this study that E2F1 was highly expressed in non-proliferating pancreatic β-cells, suggesting that E2F1, besides the control of β-cell number could have a role in pancreatic β-cell function. We demonstrate in our recent study, both in vitro and in vivo that E2F1 directly regulates the expression of Kir6.2, a key component of the KATP channel involved in the regulation of glucose-induced insulin secretion in pancreatic β-cells. Expression of Kir6.2 is lost in pancreas of E2f1-/- mice, resulting in insulin secretion defects in these mice. Furthermore, we demonstrated by in tissue chromatin immunoprecipitation analysis that regulation of Kir6.2 expression by E2F1 follows the same regulatory pathway that the classical E2F1 target genes, implicating the participation of CDK4 and retinoblastoma protein. Moreover, in this context, E2F1 transcriptional activity is regulated by glucose and insulin through the CDK4-dependent inactivation of the pRB protein. In summary we provide evidence that the CDK4-pRB-E2F1 regulatory pathway is involved in glucose homeostasis. In our recent study we decipher a new function for these factors in the control of insulin secretion and open up new avenues for the treatment of metabolic diseases, in particular type 2 diabetes.

  15. Multicentric evaluation of a new assay for prothrombin fragment F1+2 determination.

    Science.gov (United States)

    Bruhn, H D; Conard, J; Mannucci, M; Monteagudo, J; Pelzer, H; Reverter, J C; Samama, M; Tripodi, A; Wagner, C

    1992-10-01

    A multicenter study of a recently developed ELISA for the determination of prothrombin fragment F1+2 was performed in order to evaluate analytical and clinical aspects. Mean intra-assay and inter-assay reproducibility were found to be 11.0 and 12.6%, respectively. The measuring range covered by the calibration curve reaches from 0.04 to 10.0 nM/l F1+2. Testing 133 healthy subjects a reference range of 0.37 to 1.11 nM/l F1+2 (2.5-97.5 percentile) with a median of 0.66 nM/l F1+2 was calculated. Minor difficulties with blood sampling (venous occlusion for 2 min) did not affect F1+2 plasma concentrations. Significantly increased F1+2 levels were measured in patients with leukemia (p < 0.0001), severe liver disease (p < 0.005) and after myocardial infarction (p < 0.01). Elevated F1+2 concentration before the beginning of heparin therapy (1.25 nM/l) decreased to 0.77 nM/l (p < 0.0001) after 1 day of therapy. For patients in the stable phase of oral anticoagulant therapy decreasing F1+2 concentrations were measured with increasing INR. F1+2 levels were already significantly reduced in patients with INR < 2.0 (0.56 nM/l; p = 0.0005). Thus F1+2 determination may be helpful in identifying activation processes as well as in monitoring anticoagulant therapy.

  16. The Polymorphism of Pituitary Factor 1 (POU1F1 in Cattle

    Directory of Open Access Journals (Sweden)

    Teodora Crina Carsai

    2012-05-01

    Full Text Available The development and function of mammary gland is mainly controlled by growth hormone and prolactin, twoprotein hormones secreted by the anterior pituitary gland. Their synthesis is under regulatory influence of pituitaryfactor 1 (PIT1 or POU1F1, a protein factor produced in hypothalamic nuclei. In cattle, it was shown that a HinfIpolymorphism located in exon 6 of PIT1 gene may have significant influence on milk quantity. In particular A allelewas associated with a higher milk yield and could be a valuable genetic marker for improving milk quantity in cattle.In an effort to better understand the possible influence of this polymorphism on mammary gland development andfunction in cattle, we have studied the frequency this polymorphism in Romanian Black and White breed, a highmilk production cattle breed versus Romanian Grey Steppe breed, a primitive breed with very low milk production.In both breeds the frequency of B allele is much higher as compared with the frequency of A allele. The study ofPIT1 polymorphism in Romanian cattle breeds is a part of a more complex study targeting several key genesinvolved in mammary gland function.

  17. Use of hybridization (F1 in forage sorghum (Sorghum bicolor (L. Moench breeding

    Directory of Open Access Journals (Sweden)

    Pataki Imre

    2010-01-01

    Full Text Available In plants with bisexual flowers, the development of hybrids and F1 seed production is only possible by using cytoplasmatic male sterility. The discovery of such sterility and the maintainers has made it possible to utilize the phenomenon of heterosis to improve yields and yield components in forage sorghum. It has been shown that the best way to develop forage sorghum hybrids is to cross grain sorghum as the female parent and Sudan grass as the male. The objective of this study was to develop a forage sorghum hybrid for the production of green matter to be used either fresh or for silage. The sorghum hybrid developed in these efforts (Siloking is intended for multiple cutting, as the basal nodes produce buds and regrowth takes place. The performance of the new hybrid with respect to yield and quality was compared to that of the forage sorghum cultivar NS Džin. In a two-year study conducted under different growing conditions in four locations, Siloking produced an average green matter yield of 86.29 t ha-1 (two cuts, a dry matter yield of 25.34 t ha-1, and a crude protein content of 11.85 %. Siloking outperformed NS Džin in terms of yield and quality. .

  18. Optimizing the number of progenies and replications in plant breeding experiments

    Directory of Open Access Journals (Sweden)

    João Luís da Silva Filho

    2013-01-01

    Full Text Available A determination criterion was proposed for the number of replications, r, and of evaluated progenies, Nr, given P experimentalplots, with Nr=P/r, and n progenies to be selected; its application was discussed in the selection of progenies of bulk populations,derived from two homozygous parents. For a known heritability at the plot level, h20, there is a critical n below which the gain isgreater with selection evaluating P/(r+1 progenies in r+1 than P/r progenies in r replications. Different h20 scenarios were simulatedin the F2 and F∞ generations, assuming no dominance. It was demonstrated that at any h20 , if n > 18.5% of P, larger gains are obtainedby assuming Nr = P, showing that the augmented block design could be used in the early stages of breeding programs. The higher h20,the higher must be the selection intensity to justify the use of additional replications.

  19. Genetic variability in progenies of Eucalyptus dunnii Maiden for resistance to Puccinia psidii

    Directory of Open Access Journals (Sweden)

    Cleber da Silva Pinto

    2014-10-01

    Full Text Available This study investigated the genetic variability in progenies of Eucalyptus dunnii Maiden for resistance against rust (Puccinia psidii. Field experiments were installed in two regions with different soil-climatic conditions. Open-pollinated progenies were established in a randomized complete block design. Sixty and 48 progenies were evaluated under field conditions at two sites, respectively, with six replications and eight trees per plot. In another experiment in a controlled environment, 53 progenies were evaluated in randomized blocks with six replications and nine plants per plot. The following traits were evaluated: plant height, severity of pest attack and the most susceptible stage to the leaf disease. The genetic variability for rust resistance in the E. dunnii population under study was high, with a genetic coefficient of variation of 36.07%; 7% of the evaluated progenies were rust-resistant. It indicates a high potential for selection and breeding of the species.

  20. Treponema pallidum (syphilis) antigen TpF1 induces angiogenesis through the activation of the IL-8 pathway.

    Science.gov (United States)

    Pozzobon, Tommaso; Facchinello, Nicola; Bossi, Fleur; Capitani, Nagaja; Benagiano, Marisa; Di Benedetto, Giulietta; Zennaro, Cristina; West, Nicole; Codolo, Gaia; Bernardini, Marialina; Baldari, Cosima Tatiana; D'Elios, Mario Milco; Pellegrini, Luca; Argenton, Francesco; de Bernard, Marina

    2016-01-05

    Over 10 million people every year become infected by Treponema pallidum and develop syphilis, a disease with broad symptomatology that, due to the difficulty to eradicate the pathogen from the highly vascularized secondary sites of infection, is still treated with injections of penicillin. Unlike most other bacterial pathogens, T. pallidum infection produces indeed a strong angiogenic response whose mechanism of activation, however, remains unknown. Here, we report that one of the major antigen of T. pallidum, the TpF1 protein, has growth factor-like activity on primary cultures of human endothelial cells and activates specific T cells able to promote tissue factor production. The growth factor-like activity is mediated by the secretion of IL-8 but not of VEGF, two known angiogenic factors. The pathogen's factor signals IL-8 secretion through the activation of the CREB/NF-κB signalling pathway. These findings are recapitulated in an animal model, zebrafish, where we observed that TpF1 injection stimulates angiogenesis and IL-8, but not VEGF, secretion. This study suggests that the angiogenic response observed during secondary syphilis is triggered by TpF1 and that pharmacological therapies directed to inhibit IL-8 response in patients should be explored to treat this disease.

  1. Reproductive effects in F1 adult females exposed in utero to moderate to high doses of mono-2-ethylhexylphthalate (MEHP).

    Science.gov (United States)

    Moyer, Benjamin; Hixon, Mary L

    2012-08-01

    Phthalates are widely used as plasticizers in everyday products. Yet, studies on the effects of phthalates on female reproductive health are limited. In this study, pregnant C57/Bl6 mice were exposed via oral gavage to corn oil, 100, 500, or 1000mg/kg MEHP from gestational days 17-19. Reproductive lifespan was decreased by one month in the highest F1 exposure group (9.8±0.4 versus 11.1±0.6 months in control F1 females). F1 females exhibited delayed estrous onset at the two higher exposures and prolonged estrus was observed in all MEHP-exposed females. Serum FSH and estradiol were significantly elevated at the highest exposure and altered mRNA expression was found for the steroidogenic genes LHCGR, aromatase, and StAR. At one year of age, mammary gland hyperplasia was observed in high dose MEHP-exposed females. In summary, late gestational exposure to MEHP leads to multiple latent reproductive effects throughout murine life resulting in premature ovarian senescence and mammary hyperplasia.

  2. Development of an integrated sampler based on direct {sup 222}Rn/{sup 220}Rn progeny sensors in flow-mode for estimating unattached/attached progeny concentration

    Energy Technology Data Exchange (ETDEWEB)

    Mishra, Rosaline, E-mail: rosaline@barc.gov.i [Radiological Physics and Advisory Division, Health, Safety and Environment Group, Bhabha Atomic Research Centre, Mumbai 400 085 (India); Sapra, B.K.; Mayya, Y.S. [Radiological Physics and Advisory Division, Health, Safety and Environment Group, Bhabha Atomic Research Centre, Mumbai 400 085 (India)

    2009-11-15

    A flow-mode integrated sampler consisting of a wire-mesh and filter-paper array along with passive solid state nuclear track detectors has been developed for estimating unattached and attached fraction of {sup 222}Rn/{sup 220}Rn progeny concentration. The essential element of this sampler is the direct {sup 222}Rn/{sup 220}Rn progeny sensor (DRPS/DTPS), which is an absorber-mounted-LR115 type nuclear track detector that selectively registers the alpha particles emitted from the progeny deposited on its surface. During sampling at a specified flow-rate, the unattached progeny is captured on the wire-mesh; while the attached progeny gets transmitted and is captured on the filter-paper. The alpha particles emitted by the deposited progeny atoms are registered on the sensors placed at a specified distance facing the wire-mesh and the filter-paper, respectively. The various steps involved in the development of this flow-mode direct progeny sampler such as the optimization of the sampling rate and the distance between the sensor and the deposition substrate are discussed. The sensitivity factor of the DTPS-loaded sampler for {sup 220}Rn progeny deposited on the wire-mesh and filter-paper is found to be 23.77 +- 0.64 (track cm{sup -2} h{sup -1}) (Bq m{sup -3}){sup -1} and 22.30 +- 0.18 (track cm{sup -2} h{sup -1}) (Bq m{sup -3}){sup -1}, respectively; while that of DRPS-loaded sampler for {sup 222}Rn progeny deposition, is 3.03 +- 0.14 (track cm{sup -2} h{sup -1}) (Bq m{sup -3}){sup -1} and 2.08 +- 0.07 (track cm{sup -2} h{sup -1}) (Bq m{sup -3}){sup -1}, respectively. The highlight of this flow-mode sampler is its high sensitivity and that it utilizes the passive technique for estimating the unattached and attached progeny concentration, thus doing away with the alpha counting procedures.

  3. Alteration of mitochondrial genomes containing atpA genes in the sexual progeny of cybrids between Raphanus sativus cms line and Brassica napus cv. Westar.

    Science.gov (United States)

    Sakai, T; Imamura, J

    1992-09-01

    We have investigated the fate of the mitochondrial genomes of cybrids derived from "donor-recipient" protoplast fusion between X-irradiated Raphanus sativus (cms line) and iodoacetamide-treated Brassica napus cv. Westar. Two out of ten fusion products were male-sterile with the diploid chromosome number of B. napus. The mitochondrial (mt) genomes of the cybrids and their progeny were further analyzed by DNA-DNA hybridizaion using the pea mitochondrial ATPase subunit gene (atpA) as a probe. One cybrid, 18-3, had a 3.0 kb fragment characteristic of B. napus and a 2.0 kb non-parental fragment when the BamHI-digested DNA was hybridized with the probe. In the first-backcrossed progeny of this cybrid, the hybridization pattern was not stably inherited. A 4.0 kb radish fragment, not detectable in the cybrid, appeared in one of the BC1 generation siblings, and the 2.0 kb non-parental fragment was lost in another. The hybridization patterns in BC1 progeny siblings of cybrid 12-9 were also varied. The alteration of mtDNA in the cybrid progeny continued to the BC2 generation. There was no clear evidence of a heteroplasmic state or of sub-stoichiometric molecules in the mt genome of cybrid 18-3. A possible cause of the observed alteration in the mt genome is discussed.

  4. Rotation of subunits during catalysis by Escherichia coli F1-ATPase.

    Science.gov (United States)

    Duncan, T M; Bulygin, V V; Zhou, Y; Hutcheon, M L; Cross, R L

    1995-11-21

    During oxidative and photo-phosphorylation, F0F1-ATP synthases couple the movement of protons down an electrochemical gradient to the synthesis of ATP. One proposed mechanistic feature that has remained speculative is that this coupling process requires the rotation of subunits within F0F1. Guided by a recent, high-resolution structure for bovine F1 [Abrahams, J. P., Leslie, A. G., Lutter, R. & Walker, J. E. (1994) Nature (London) 370, 621-628], we have developed a critical test for rotation of the central gamma subunit relative to the three catalytic beta subunits in soluble F1 from Escherichia coli. In the bovine F1 structure, a specific point of contact between the gamma subunit and one of the three catalytic beta subunits includes positioning of the homolog of E. coli gamma-subunit C87 (gamma C87) close to the beta-subunit 380DELSEED386 sequence. A beta D380C mutation allowed us to induce formation of a specific disulfide bond between beta and gamma C87 in soluble E. coli F1. Formation of the crosslink inactivated beta D380C-F1, and reduction restored full activity. Using a dissociation/reassembly approach with crosslinked beta D380C-F1, we incorporated radiolabeled beta subunits into the two noncrosslinked beta-subunit positions of F1. After reduction of the initial nonradioactive beta-gamma crosslink, only exposure to conditions for catalytic turnover results in similar reactivities of unlabeled and radiolabeled beta subunits with gamma C87 upon reoxidation. The results demonstrate that gamma subunit rotates relative to the beta subunits during catalysis.

  5. Absence of pRb facilitates E2F1-induced apoptosis in breast cancer cells.

    Science.gov (United States)

    Sun, Baohua; Wingate, Hannah; Swisher, Stephen G; Keyomarsi, Khandan; Hunt, Kelly K

    2010-03-15

    The transcription factor E2F1 is known for its interaction with pRb, controlling cell proliferation; however, E2F1 also has a pivotal role in regulating apoptosis.  The relationship between pRb and E2F1 balances cell proliferation and apoptosis giving pRb tumor suppressive properties. The intricacies of the pRb/E2F1 relationship and thus the regulation of cell fate is cell context dependent. To explore the role of pRb in the E2F1-induced apoptosis of human breast cancer cells, we examined cell growth and apoptosis induction in isogenic cell systems of immortalized breast epithelial cells lacking either pRb (76NE7) or p53 (76NE6). We found that E2F1 caused accumulation of cells in G2 and S phases of the cell cycle along with apoptosis in 76NE7 but not 76NE6 cells.  Variants of 76NE6 cells with functional p53 did not rescue the apoptotic response in these cells, whereas knocking down pRb resulted in significant E2F1-induced apoptosis. We also determined that the effect of E2F1 overexpression in two breast cancer cell lines, MDA-MB-436 and MDA-MB-468, which lack pRb and functional p53, was accumulation of cells in G2/S phase and apoptosis. However, E2F did not cause apotosis  in MCF-7 cells which harbor a functional pRb. Therefore, we conclude that in the absence of Rb, E2F1 overexpression results in apoptosis, not proliferation, and that this effect is independent of p53.

  6. 17 CFR 270.18f-1 - Exemption from certain requirements of section 18(f)(1) (of the Act) for registered open-end...

    Science.gov (United States)

    2010-04-01

    ... requirements of section 18(f)(1) (of the Act) for registered open-end investment companies which have the right... which have the right to redeem in kind. (a) A registered open-end investment company which has the right... to pay in cash all requests for redemption by any shareholder of record, limited in amount...

  7. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2004-130

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 1607-F1 Sanitary Sewer System (124-F-1), consisted of a septic tank, drain field, and associated pipelines that received sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office via the 100-F-26:8 pipelines. The septic tank required remedial action based on confirmatory sampling. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River.

  8. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2004-130

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 1607-F1 Sanitary Sewer System (124-F-1), consisted of a septic tank, drain field, and associated pipelines that received sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office via the 100-F-26:8 pipelines. The septic tank required remedial action based on confirmatory sampling. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River.

  9. Selecting elephant grass families and progenies to produce bioenergy through mixed models (REML/BLUP).

    Science.gov (United States)

    Rodrigues, E V; Daher, R F; Dos Santos, A; Vivas, M; Machado, J C; Gravina, G do A; de Souza, Y P; Vidal, A K; Rocha, A Dos S; Freitas, R S

    2017-05-18

    Brazil has great potential to produce bioenergy since it is located in a tropical region that receives high incidence of solar energy and presents favorable climatic conditions for such purpose. However, the use of bioenergy in the country is below its productivity potential. The aim of the current study was to select full-sib progenies and families of elephant grass (Pennisetum purpureum S.) to optimize phenotypes relevant to bioenergy production through mixed models (REML/BLUP). The circulating diallel-based crossing of ten elephant grass genotypes was performed. An experimental design using the randomized block methodology, with three repetitions, was set to assess both the hybrids and the parents. Each plot comprised 14-m rows, 1.40 m spacing between rows, and 1.40 m spacing between plants. The number of tillers, plant height, culm diameter, fresh biomass production, dry biomass rate, and the dry biomass production were assessed. Genetic-statistical analyses were performed through mixed models (REML/BLUP). The genetic variance in the assessed families was explained through additive genetic effects and dominance genetic effects; the dominance variance was prevalent. Families such as Capim Cana D'África x Guaçu/I.Z.2, Cameroon x Cuba-115, CPAC x Cuba-115, Cameroon x Guaçu/I.Z.2, and IAC-Campinas x CPAC showed the highest dry biomass production. The family derived from the crossing between Cana D'África and Guaçu/I.Z.2 showed the largest number of potential individuals for traits such as plant height, culm diameter, fresh biomass production, dry biomass production, and dry biomass rate. The individual 5 in the family Cana D'África x Guaçu/I.Z.2, planted in blocks 1 and 2, showed the highest dry biomass production.

  10. Rotation of subunits during catalysis by Escherichia coli F1-ATPase.

    OpenAIRE

    1995-01-01

    During oxidative and photo-phosphorylation, F0F1-ATP synthases couple the movement of protons down an electrochemical gradient to the synthesis of ATP. One proposed mechanistic feature that has remained speculative is that this coupling process requires the rotation of subunits within F0F1. Guided by a recent, high-resolution structure for bovine F1 [Abrahams, J. P., Leslie, A. G., Lutter, R. & Walker, J. E. (1994) Nature (London) 370, 621-628], we have developed a critical test for rotation ...

  11. Optical pumping effect in absorption imaging of F=1 atomic gases

    CERN Document Server

    Kim, Sooshin; Noh, Heung-Ryoul; Shin, Y

    2016-01-01

    We report our study of the optical pumping effect in absorption imaging of $^{23}$Na atoms in the $F=1$ hyperfine spin states. Solving a set of rate equations for the spin populations under a probe beam, we obtain an analytic expression for the optical signal of the $F=1$ absorption imaging. Furthermore, we verify the result by measuring the absorption spectra of $^{23}$Na Bose-Einstein condensates prepared in various spin states with different probe beam pulse durations. The analytic result can be used in quantitative analysis of $F=1$ spinor condensate imaging and readily applied to other alkali atoms with $I=3/2$ nuclear spin such as $^{87}$Rb.

  12. ESR-spektroskopische Untersuchungen der F0F1-ATP-Synthase aus Escherichia coli

    OpenAIRE

    Motz, Christian

    1999-01-01

    Die FoF1-ATP-Synthase katalysiert die Synthese von ATP aus ADP und Pi bei der oxidativen bzw. Photophosphorylierung. Der ATP-Synthase-Komplex läßt sich in zwei funktionelle Einheiten unterteilen: Fo ist ein integraler Membranproteinkomplex, der den Protonenkanal bildet. F1 hingegen ist ein wasserlöslicher Proteinkomplex, der die Nukleotidbindungsstellen trägt. Die ATP-Synthase aus Escherichia coli hat die Zusammensetzung alpha3beta3gamma delta epsilon für die F1 und ab2c9-12 für den Fo-Teil. ...

  13. Measurment of radon, thoron and their progeny in indoor environment of Mohali, Punjab, Northern India, using pinhole dosimeters

    Directory of Open Access Journals (Sweden)

    Mehta Vimal

    2016-01-01

    Full Text Available The health hazards of radon and its decay products above certain levels are well known. However, for any preventive measures to be taken, we have to be aware of radon levels of that particular area. Measurement of radon and its decay products in indoor environments is an important aspect of assessing indoor air quality and health conditions associated with it. Keeping this in mind, measurements of radon, thoron and their progeny concentrations were carried out in Mohali, Northern India, using pinhole-based twin cup dosimeters. Radon exhalation rates of soil samples in the dwellings/areas were measured via an active technique of a continuous radon monitor. The indoor radon concentration in Mohali varied from 15.03 ± 0.61 Bq/m3 to 39.21 ± 1.46 Bq/m3 with an average of 26.95 Bq/m3 ,while thoron concentration in the same dwellings varied from 9.62 ± 0.54 Bq/m3 to 52.84 ± 2.77 Bq/m3 with an average of 31.09 Bq/m3. Radon progeny levels in dwellings under study varied from 1.63 to 4.24 mWL, with an average of 2.94 mWL, while thoron progeny levels varied from 0.26 to 1.43 mWL , with an average of 0.84 mWL. The annual dose received by the inhabitants of dwellings under study varied from 0.78 to 2.36 mSv, with an average of 1.61 mSv. The in situ gamma dose rate varied from 0.12 to 0.32 mSv/h.

  14. Characterization of an F1 deletion mutant of Yersinia pestis CO92, pathogenic role of F1 antigen in bubonic and pneumonic plague, and evaluation of sensitivity and specificity of F1 antigen capture-based dipsticks.

    Science.gov (United States)

    Sha, Jian; Endsley, Janice J; Kirtley, Michelle L; Foltz, Sheri M; Huante, Matthew B; Erova, Tatiana E; Kozlova, Elena V; Popov, Vsevolod L; Yeager, Linsey A; Zudina, Irina V; Motin, Vladimir L; Peterson, Johnny W; DeBord, Kristin L; Chopra, Ashok K

    2011-05-01

    We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipsticks, an in-frame F1-deficient mutant of CO92 (Δcaf) was generated by homologous recombination and used as a negative control. Based on genetic, antigenic/immunologic, and electron microscopic analyses, the Δcaf mutant was devoid of a capsule. The growth rate of the Δcaf mutant generally was similar to that of the wild-type (WT) bacterium at both 26 and 37 °C, although the mutant's growth dropped slightly during the late phase at 37 °C. The Δcaf mutant was as virulent as WT CO92 in the pneumonic plague mouse model; however, it was attenuated in developing bubonic plague. Both dipsticks had similar sensitivities, requiring a minimum of 0.5 μg/ml of purified F1 antigen or 1 × 10(5) to 5 × 10(5) CFU/ml of WT CO92 for positive results, while the blood samples were negative for up to 1 × 10(8) CFU/ml of the Δcaf mutant. Our studies demonstrated the diagnostic potential of two plague dipsticks in detecting capsular-positive strains of Y. pestis in bubonic and pneumonic plague.

  15. Selection of cowpea progenies with enhanced drought-tolerance traits using principal component analysis.

    Science.gov (United States)

    Sousa, C C; Damasceno-Silva, K J; Bastos, E A; Rocha, M M

    2015-12-07

    Vigna unguiculata (L.) Walp (cowpea) is a food crop with high nutritional value that is cultivated throughout tropical and subtropical regions of the world. The main constraint on high productivity of cowpea is water deficit, caused by the long periods of drought that occur in these regions. The aim of the present study was to select elite cowpea genotypes with enhanced drought tolerance, by applying principal component analysis to 219 first-cycle progenies obtained in a recurrent selection program. The experimental design comprised a simple 15 x 15 lattice with 450 plots, each of two rows of 10 plants. Plants were grown under water-deficit conditions by applying a water depth of 205 mm representing one-half of that required by cowpea. Variables assessed were flowering, maturation, pod length, number and mass of beans/pod, mass of 100 beans, and productivity/plot. Ten elite cowpea genotypes were selected, in which principal components 1 and 2 encompassed variables related to yield (pod length, beans/pod, and productivity/plot) and life precocity (flowering and maturation), respectively.

  16. Levels of thoron and progeny in high background radiation area of southeastern coast of Odisha, India.

    Science.gov (United States)

    Ramola, R C; Gusain, G S; Rautela, B S; Sagar, D V; Prasad, G; Shahoo, S K; Ishikawa, T; Omori, Y; Janik, M; Sorimachi, A; Tokonami, S

    2012-11-01

    Exposure to radon, (222)Rn, is assumed to be the most significant source of natural radiation to human beings in most cases. It is thought that radon and its progeny are major factors that cause cancer. The presence of thoron, (220)Rn, was often neglected because it was considered that the quantity of thoron in the environment is less than that of radon. However, recent studies have shown that a high thoron concentration was found in some regions and the exposure to (220)Rn and its progeny can equal or several time exceed that of (220)Rn and its progeny. The results of thoron and its progeny measurements in the houses of high background radiation area (HBRA) of the southeastern coast of Odisha, India presented here. This area is one of the high background radiation areas in India with a large deposit of monazite sand which is the probable source of thoron. Both active and passive methods were employed for the measurement of thoron and its progeny in cement, brick and mud houses in the study area. Thoron concentration was measured using RAD-7 and Raduet. A CR-39 track detector was employed for the measurement of environmental thoron progeny, both in active and passive modes. Thoron and its progeny concentrations were found to be comparatively high in the area. A comparison between the results obtained with various techniques is presented in this paper.

  17. Parental Drought and Defoliation Effect on Yield, Grains Biochemical Aspects and Drought Performance of Sorghum Progeny

    Directory of Open Access Journals (Sweden)

    Ibrahim, A. H.

    2013-02-01

    Full Text Available This study was designed to evaluate the effect of drought stress and drought with defoliation on yield parameters of three sorghum varieties (Giza 15, Dorado and Hybrid 113. Also, the effect of these parental stress treatments on drought performance of progeny of the most drought tolerant variety was investigated. Application of drought stress in the vegetative stage non significantly affected panicles number, grain yield and harvest index of all cultivars. Drought stress in the reproductive stage of Giza 15 and Hybrid 113 cultivars caused a two fold increase in length of lateral branch and panicles number. However, grain yield and total panicles weight were significantly reduced in all cultivars due to this stress. Application of drought with defoliation in the vegetative stage reduced shoot and straw weights, and grain yield in sorghum in comparison with drought stress only. Protein-N and polysaccharides content were decreased in parent grains in response to water stress.The stress intensity index (SII of progeny from drought- subjected parents was about 30-fold greater than SII of progeny from control parents. Further, SII of progeny from parents exposed to drought stress in the reproductive stage was higher than the SII of progeny from parents subjected to drought stress in the vegetative stage . A strong negative correlation appeared between the stress intensity index of the progeny and polysaccharides content of parent grains. Based on our research parental defoliation did not improve the drought resistance of sorghum progeny.

  18. EVALUATION OF THE IMMUNOMODULATORY EFFECTS OF THE DISINFECTION BYPRODUCT, SODIUM CHLORITE, IN FEMALE B6C3F1 MICE: A DRINKING WATER STUDY

    Science.gov (United States)

    Evaluation of the Immunomodulatory Effects of the Disinfection By-product, Sodium chlorite, in Female B6C3f1 mice: A Drinking Water Study. Niel A. Karrow, Tal, L. Guo, J. Ann McCay, Greg W. Johnson, Ronnetta D. Brown, Debrorah L. Musgrove, Dori R. Germolec, Robert W. Lueb...

  19. Effect of type and concentration of water hardness on the fertilization and hatching success of channel X blue F1 hybrid catfish eggs

    Science.gov (United States)

    Consistent and improved performance of channel x blue F1 hybrid catfish fingerlings in production ponds in the US farm raised catfish industry has prompted an increase in demand of hybrid catfish fingerlings even at higher prices compared to commonly raised channel catfish. Hybrid catfish fry are e...

  20. Zinc distribution and speciation in Arabidopsis halleri x Arabidops is lyrata progenies presenting various zinc accumulation capacities

    Energy Technology Data Exchange (ETDEWEB)

    Sarret, Geraldine; Willems, Glenda; Isaure, Marie-Pierre; Marcus, Matthew A.; Fakra, Sirine C.; Frerot, Helene; Pairis, Sebastien; Geoffroy, Nicolas; Manceau, Alain; Saumitou-Laprade, Pierre

    2010-04-08

    - The purpose of this study was to investigate the relationship between the chemical form and localization of zinc (Zn) in plant leaves and their Zn accumulationcapacity. - An interspecific cross between Arabidopsis halleri sp. halleri and Arabidopsis lyrata sp. petrea segregating for Zn accumulation was used. Zinc (Zn) speciation and Zn distribution in the leaves of the parent plants and of selected F1 and F2 progenies were investigated by spectroscopic and microscopic techniques and chemical analyses. - A correlation was observed between the proportion of Zn being in octahedral coordination complexed to organic acids and free in solution (Zn?OAs + Znaq) and Zn content in the leaves. This pool varied between 40percent and 80percent of total leaf Zn depending on the plant studied. Elemental mapping of the leaves revealed different Zn partitioning between the veins and the leaf tissue. The vein : tissue fluorescence ratio was negatively correlated with Zn accumulation. - The higher proportion of Zn?OAs + Znaq and the depletion of the veins in the stronger accumulators are attributed to a higher xylem unloading and vacuolar sequestration in the leaf cells. Elemental distributions in the trichomes were also investigated, and results support the role of carboxyl and⁄ or hydroxyl groups as major Zn ligands in these cells.

  1. Phenotypic variation of F1 and F2 populations from three species of ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-07-18

    Jul 18, 2008 ... Phenotypic variation of F1 and F2 populations from ... Key words: Solanum, genome, phenotype, taxonomy, evolution, interspecific hybridization, pollen viability, ..... This development affirms the views of .... The origins of.

  2. Maternal Diet and Insulin-Like Signaling Control Intergenerational Plasticity of Progeny Size and Starvation Resistance.

    Directory of Open Access Journals (Sweden)

    Jonathan D Hibshman

    2016-10-01

    Full Text Available Maternal effects of environmental conditions produce intergenerational phenotypic plasticity. Adaptive value of these effects depends on appropriate anticipation of environmental conditions in the next generation, and mismatch between conditions may contribute to disease. However, regulation of intergenerational plasticity is poorly understood. Dietary restriction (DR delays aging but maternal effects have not been investigated. We demonstrate maternal effects of DR in the roundworm C. elegans. Worms cultured in DR produce fewer but larger progeny. Nutrient availability is assessed in late larvae and young adults, rather than affecting a set point in young larvae, and maternal age independently affects progeny size. Reduced signaling through the insulin-like receptor daf-2/InsR in the maternal soma causes constitutively large progeny, and its effector daf-16/FoxO is required for this effect. nhr-49/Hnf4, pha-4/FoxA, and skn-1/Nrf also regulate progeny-size plasticity. Genetic analysis suggests that insulin-like signaling controls progeny size in part through regulation of nhr-49/Hnf4, and that pha-4/FoxA and skn-1/Nrf function in parallel to insulin-like signaling and nhr-49/Hnf4. Furthermore, progeny of DR worms are buffered from adverse consequences of early-larval starvation, growing faster and producing more offspring than progeny of worms fed ad libitum. These results suggest a fitness advantage when mothers and their progeny experience nutrient stress, compared to an environmental mismatch where only progeny are stressed. This work reveals maternal provisioning as an organismal response to DR, demonstrates potentially adaptive intergenerational phenotypic plasticity, and identifies conserved pathways mediating these effects.

  3. Characterization of the myelotoxicity of chloramphenicol succinate in the B6C3F1 mouse.

    Science.gov (United States)

    Turton, John A; Fagg, Rajni; Sones, William R; Williams, Thomas C; Andrews, C Michael

    2006-04-01

    Chloramphenicol (CAP) is haemotoxic in man, inducing two types of toxicity. First, a dose-related, reversible anaemia with reticulocytopenia, sometimes seen in conjunction with leucopenia and thrombocytopenia; this form of toxicity develops during drug treatment. The second haemotoxicity is aplastic anaemia (AA) which is evident in the blood as severe pancytopenia. AA development is not dose-related and occurs weeks or months after treatment. We wish, in the longer term, to investigate CAP-induced AA in the busulphan-pretreated mouse. However, as a prelude to that study, we wanted to characterize in detail the reversible haemotoxicity of CAP succinate (CAPS), administered at high dose levels in the mouse, and follow the recovery of the bone marrow in the post-dosing period. Female B6C3F1 mice were gavaged with CAPS at 0, 2500 and 3500 mg/kg, daily, for 5 days and sampled (n = 5) at 1, 7, 14 and 21 days post-dosing. Blood, bone marrow and spleen samples were analysed and clonogenic assays carried out. At day 1 post-dosing, at both CAPS dose levels, decreases were seen in erythrocytes and erythrocyte precursors; marrow erythroid cells were reduced. Reductions were also evident in splenic nucleated cell counts, blood high fluorescence ratio (HFR) reticulocyte counts and total reticulocyte counts; burst-forming units-erythroid and colony-forming units-erythroid showed decreases. At day 7 post-dosing (2500 mg/kg CAPS), there was regeneration of erythrocyte production, with marked splenic erythropoietic activity, and raised blood HFR reticulocytes. At day 7, at 3500 mg/kg CAPS, erythrocyte and reticulocyte parameters remained depressed. At 14 days post-dosing (2500 mg/kg CAPS), many erythrocyte parameters had returned to normal; at 3500 mg/kg CAPS, there was erythroid regeneration. By 21 days post-dosing, at both CAPS dose levels, most erythrocytic parameters were equivalent to control values. For leucocyte parameters, there was some depression at day 1 post-dosing (at

  4. Arabidopsis RabF1 (ARA6) Is Involved in Salt Stress and Dark-Induced Senescence (DIS)

    Science.gov (United States)

    Yin, Congfei; Karim, Sazzad; Zhang, Hongsheng; Aronsson, Henrik

    2017-01-01

    Arabidopsis small GTPase RabF1 (ARA6) functions in endosomal vesicle transport and may play a crucial role in recycling and degradation of molecules, thus involved in stress responses. Here we have reported that complementary overexpression lines RabF1OE (overexpression), GTPase mutants RabF1Q93L (constitutively active) and RabF1S47N (dominant negative) lines show longer root growth than wild-type, rabF1 knockout and N-myristoylation deletion (Δ1−29, N-terminus) complementary overexpression mutant plants under salt induced stress, which indicates that N-myristoylation of RabF1 is indispensable for salt tolerance. Moreover, RabF1 is highly expressed during senescence and RabF1OE lines were more tolerant of dark-induced senescence (DIS) than wild-type and rabF1. PMID:28157156

  5. Repression of androgen receptor transcription through the E2F1/DNMT1 axis.

    Directory of Open Access Journals (Sweden)

    Conrad David Valdez

    Full Text Available Although androgen receptor (AR function has been extensively studied, regulation of the AR gene itself has been much less characterized. In this study, we observed a dramatic reduction in the expression of androgen receptor mRNA and protein in hyperproliferative prostate epithelium of keratin 5 promoter driven E2F1 transgenic mice. To confirm an inhibitory function for E2F1 on AR transcription, we showed that E2F1 inhibited the transcription of endogenous AR mRNA, subsequent AR protein, and AR promoter activity in both human and mouse epithelial cells. E2F1 also inhibited androgen-stimulated activation of two AR target gene promoters. To elucidate the molecular mechanism of E2F-mediated inhibition of AR, we evaluated the effects of two functional E2F1 mutants on AR promoter activity and found that the transactivation domain appears to mediate E2F1 repression of the AR promoter. Because DNMT1 is a functional intermediate of E2F1 we examined DNMT1 function in AR repression. Repression of endogenous AR in normal human prostate epithelial cells was relieved by DNMT1 shRNA knock down. DNMT1 was shown to be physically associated within the AR minimal promoter located 22 bps from the transcription start site; however, methylation remained unchanged at the promoter regardless of DNMT1 expression. Taken together, our results suggest that DNMT1 operates either as a functional intermediary or in cooperation with E2F1 inhibiting AR gene expression in a methylation independent manner.

  6. 26 CFR 31.3121(f)-1 - American vessel and aircraft.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 15 2010-04-01 2010-04-01 false American vessel and aircraft. 31.3121(f)-1... § 31.3121(f)-1 American vessel and aircraft. (a) The term “American vessel” means any vessel which is...”, see § 31.3121 (e)-1.) (b) The term “American aircraft” means any aircraft registered under the laws of...

  7. 26 CFR 1.669(f)-1A - Character of capital gain.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Character of capital gain. 1.669(f)-1A Section 1... Before January 1, 1969 § 1.669(f)-1A Character of capital gain. Amounts distributed as a capital gain... the gain had with respect to the trust. Thus, a capital gain that was taxed to the trust as a...

  8. 新西兰Hulme F1公路超级跑车

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    新西兰Kiwi公司开发的 F1公路超级跑车将于今年登场。该车以1967年F1大奖赛冠军得主新西兰人Denny Hulme名字命名为Hulme。它用了两年时间进行设计和打造。

  9. Occurrence of S and F1C/S-related fimbrial determinants and their expression in Escherichia coli strains isolated from extraintestinal infections.

    Science.gov (United States)

    Sokolowska-Köhler, W; Schönian, G; Bollmann, R; Schubert, A; Parschau, J; Seeberg, A; Presber, W

    1997-05-01

    The presence of S and F1C/S-related fimbrial determinants was determined in 462 E. coli strains obtained from different extraintestinal infections and in 162 control isolates of E. coli by using two different DNA probes: an oligonucleotide probe consisting of three oligonucleotides that bind specifically to the S adhesin gene and a polynucleotide probe which is not able to distinguish between S, F1C, and S-related sequences. The expression of S and F1C phenotypes was tested by dot enzyme immunoassay with the corresponding monoclonal antibodies. S fimbriae genotypes were observed more frequently in septic (25%) and urinary (12%) isolates of E. coli than in faecal and water isolates (1%) and often occurred together with O2, O6, O18 and O83 antigens. F1C/S-related fimbrial DNA was detected with a higher frequency in UTI isolates (26%) than in septic (16%) and faecal (10%) isolates and was most frequently associated with O4, O6, and O75 serotypes. Since the production of S and F1C fimbriae was comparatively rare in all clinical and control isolates of E. coli, DNA hybridization assays which allow the sensitive and specific detection of fimbrial determinants even in the absence of their expression are preferable to phenotypic assays.

  10. Methods for Simultaneous Evaluation of Productivity, Adaptability and Genotypic Stability in Eucalyptus grandis Progeny Trials in the State of Sao Paulo Avaliação Simultânea de Produtividade, Adaptabilidade e Estabilidade Genotípica de Eucalyptus grandis em Distintos Ambientes do Estado de São Paulo

    Directory of Open Access Journals (Sweden)

    José Elidney Pinto Júnior

    2011-03-01

    Full Text Available

    The estimated Harmonic Mean for Genetic Values (MHVG, Relative Performance of Genetic Values (PRVG and the Harmonic Mean of Relative Performance of Genetic Values (MHPRVG were used as measures to provide and facilitate the interpretation of genotypic stability and adaptability of Eucalyptus grandis W. Hill ex Maiden. The different criteria were applied as alternatives to the simulation of selection for productivity, stability, adaptability, as well as for the joint value of these attributes together. The estimates were generated by using the mixed model, assuming genetic effects to be random. The software SELEGEN-REML/BLUP was used to evaluate progeny trials and to estimate statistical parameters. The best trees to be used in seedling and clonal orchards were identified. Fifty three half-sib families from three Australian provenances were tested in the municipalities of Mogi Guaçu, Boa Esperança do Sul and Caçapava, in the State of São Paulo. Compact family blocks designs were used, involving variable numbers of replicates, with six-trees linear plots, in a 3 x 2 m spacing. Two types of data transformation were tried in order to evaluate their efficiency in the reduction of the genetic, environmental and phenotypic variances. MHVG, PRVG and MHPRVG statistics proved to be useful in simulations for the selection of individuals with the attributes considered, providing options for choosing strategies and criteria by considering different values for the estimated genetic gains. The joint analysis of experiments in different locations confirmed that substantial gains can be obtained in DBH through individual selection based on their genetic values. This can be achieved by either thinning the trial, which leads to the formation of a seedling seed orchard or vegetative propagation of the best individuals, in order to establish a clonal seed orchard. The genetic correlation between locations indicated that a

  11. NR2F1 controls tumor cell dormancy via SOX9 and RARβ driven quiescence programs

    Science.gov (United States)

    Sosa, Maria Soledad; Parikh, Falguni; Maia, Alexandre Gaspar; Estrada, Yeriel; Bosch, Almudena; Bragado, Paloma; Ekpin, Esther; George, Ajish; Zheng, Yang; Lam, Hung-Ming; Morrissey, Colm; Chung, Chi-Yeh; Farias, Eduardo F.; Bernstein, Emily; Aguirre-Ghiso, Julio A.

    2014-01-01

    Metastases can originate from disseminated tumor cells (DTCs), which may be dormant for years before reactivation. Here we find that the orphan nuclear receptor NR2F1 is epigenetically upregulated in experimental HNSCC dormancy models and in DTCs from prostate cancer patients carrying dormant disease for 7–18 years. NR2F1-dependent dormancy is recapitulated by a co-treatment with the DNA demethylating agent 5-Aza-C and retinoic acid across various cancer types. NR2F1-induced quiescence is dependent on SOX9, RARβ and CDK inhibitors. Intriguingly, NR2F1 induces global chromatin repression and the pluripotency gene NANOG, which contributes to dormancy of DTCs in the bone marrow. When NR2F1 is blocked in vivo, growth arrest or survival of dormant DTCs is interrupted in different organs. We conclude that NR2F1 is a critical node in dormancy induction and maintenance by integrating epigenetic programs of quiescence and survival in DTCs. PMID:25636082

  12. Phylodynamics of HIV-1 subtype F1 in Angola, Brazil and Romania.

    Science.gov (United States)

    Bello, Gonzalo; Afonso, Joana Morais; Morgado, Mariza G

    2012-07-01

    The HIV-1 subtype F1 is exceptionally prevalent in Angola, Brazil and Romania. The epidemiological context in which the spread of HIV occurred was highly variable from one country to another, mainly due to the existence of a long-term civil war in Angola and the contamination of a large number of children in Romania. Here we apply phylogenetic and Bayesian coalescent-based methods to reconstruct the phylodynamic patterns of HIV-1 subtype F1 in such different epidemiological settings. The phylogenetic analyses of HIV-1 subtype F1 pol sequences sampled worldwide confirmed that most sequences from Angola, Brazil and Romania segregated in country-specific monophyletic groups, while most subtype F1 sequences from Romanian children branched as a monophyletic sub-cluster (Romania-CH) nested within sequences from adults. The inferred time of the most recent common ancestor of the different subtype F1 clades were as follow: Angola=1983 (1978-1989), Brazil=1977 (1972-1981), Romania adults=1980 (1973-1987), and Romania-CH=1985 (1978-1989). All subtype F1 clades showed a demographic history best explained by a model of logistic population growth. Although the expansion phase of subtype F1 epidemic in Angola (mid 1980s to early 2000s) overlaps with the civil war period (1975-2002), the mean estimated growth rate of the Angolan F1 clade (0.49 year(-1)) was not exceptionally high, but quite similar to that estimated for the Brazilian (0.69 year(-1)) and Romanian adult (0.36 year(-1)) subtype F1 clades. The Romania-CH subtype F1 lineage, by contrast, displayed a short and explosive dissemination phase, with a median growth rate (2.47 year(-1)) much higher than that estimated for adult populations. This result supports the idea that the AIDS epidemic that affected the Romanian children was mainly caused by the spread of the HIV through highly efficient parenteral transmission networks, unlike adult populations where HIV is predominantly transmitted through sexual route.

  13. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2005-004

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 100-F-26:8 waste site consisted of the underground pipelines that conveyed sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office to the 1607-F1 septic tank. The site has been remediated and presently exists as an open excavation. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling demonstrated that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also showed that residual contaminant concentrations are protective of groundwater and the Columbia River.

  14. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2005-004

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 100-F-26:8 waste site consisted of the underground pipelines that conveyed sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office to the 1607-F1 septic tank. The site has been remediated and presently exists as an open excavation. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling demonstrated that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also showed that residual contaminant concentrations are protective of groundwater and the Columbia River.

  15. Development of Yersinia pestis F1 antigen-loaded microspheres vaccine against plague

    Directory of Open Access Journals (Sweden)

    Huang SS

    2014-02-01

    Full Text Available Shih-shiung Huang,1 I-Hsun Li,2,3 Po-da Hong,1 Ming-kung Yeh1,2,41Biomedical Engineering Program, Graduate Institute of Engineering, Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, Republic of China; 2School of Pharmacy, 3Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China; 4Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan, Republic of ChinaAbstract: Yersinia pestis F1 antigen-loaded poly(DL-lactide-co-glycolide/polyethylene glycol (PEG (PLGA/PEG microspheres were produced using a water-in-oil-in-water emulsion/solvent extraction technique and assayed for their percent yield, entrapment efficiency, surface morphology, particle size, zeta potential, in vitro release properties, and in vivo animal protect efficacy. The Y. pestis F1 antigen-loaded microspheres (mean particle size 3.8 µm exhibited a high loading capacity (4.5% w/w, yield (85.2%, and entrapment efficiency (38.1%, and presented a controlled in vitro release profile with a low initial burst (18.5%, then continued to release Y. pestis F1 antigen over 70 days. The distribution (% of Y. pestis F1 on the microspheres surface, outer layer, and core was 3.1%, 28.9%, and 60.7%, respectively. A steady release rate was noticed to be 0.55 µg Y. pestis F1 antigen/mg microspheres/day of Y. pestis F1 antigen release maintained for 42 days. The cumulative release amount at the 1st, 28th, and 42nd days was 8.2, 26.7, and 31.0 µg Y. pestis F1 antigen/mg microspheres, respectively. The 100 times median lethal dose 50% (LD50 of Y. pestis Yokohama-R strain by intraperitoneal injection challenge in mice test, in which mice received one dose of 40 µg F1 antigen content of PLGA/PEG microspheres, F1 antigen in Al(OH3, and in comparison with F1 antigen in Al(OH3 vaccine in two doses, was evaluated after given by subcutaneous

  16. MEASUREMENT OF RADON, THORON AND THEIR PROGENY CONCENTRATIONS IN THE DWELLINGS OF PAURI GARHWAL, UTTARAKHAND, INDIA.

    Science.gov (United States)

    Joshi, Veena; Dutt, Sanjay; Yadav, Manjulata; Mishra, Rosaline; Ramola, R C

    2016-10-01

    It is well known that inhalation of radon, thoron and their progeny contributes more than 50 % of natural background radiation dose to human being. The time-integrated passive measurements of radon, thoron and their progeny concentrations were carried out in the dwellings of Pauri Garhwal, Uttarakhand, India. The measurements of radon and thoron concentrations were performed by LR-115 detector-based single-entry pin-hole dosemeter, while for the measurement of progeny concentrations, LR-115 deposition-based direct radon and thoron progeny sensors technique was used. The experimental techniques and results obtained are discussed in detail. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  17. Radon and thoron progeny concentration variability in relation to meteorological conditions at Bucharest (Romania)

    Energy Technology Data Exchange (ETDEWEB)

    Baciu, Adriana Celestina [National Commission for Nuclear Activities Control, Section for Radioprotection and Radiological Emergencies, Libertatii 14 Blvd, Bucharest 5 (Romania)]. E-mail: adriana.baciu@cncan.ro

    2005-07-01

    The paper presents results of natural radioactivity levels in the atmosphere obtained for a 5 years period (1994-1999) at the Bucharest Environmental Radioactivity Surveillance Station (BERSS). The variability of radon and thoron progeny activity concentrations is analysed in relation to the local dynamics of the meteorological parameters (wind speed, air temperature, air pressure, cloud cover, relative humidity). The radon and thoron progeny concentrations display a daily and seasonal variation, with the highest values in the early morning and the lowest values in the afternoon. The outdoor radon progeny concentrations show maximum values in autumn and minimum values in spring-summer. The outdoor thoron progeny concentrations display maximum values in autumn and minimum values in winter. Significant statistical correlations with the meteorological parameters were obtained. The study on the temporal variability of natural atmospheric radioactivity near Bucharest is a starting point for further assessment of the radiological consequences resulting from human activities.

  18. [Genetic polymorphism of clones and their seed progeny in the scotch pine clone plantation].

    Science.gov (United States)

    Korshikov, I I; Demkovich, A E

    2010-01-01

    Genetic variation at 12 allozyme loci (10 of them being polymorphic ones) has been studied in the archive-clone plantation of 23 Pinus sylvestris plus-trees and their seed progeny in the south-east of Ukraine. More than a half of clones had 4-8 heterozygous loci, whereas their seed progeny was marked by a lower variation than maternal trees. Seed progeny was obtained at a high outcrossing rate (t(m) = 95%). The clone progeny was characterized by a high percentage of abnormal allele segregation in megagametophytes. There was also a high frequency of significant deviation in distribution of seed embryo genotypes from the theoretically expected one according to the Hardy-Weinberg law.

  19. Changes to DNA methylation and homologous recombination frequency in the progeny of stressed plants.

    Science.gov (United States)

    Migicovsky, Zoë; Kovalchuk, Igor

    2013-02-01

    Plants undergo changes in response to biotic and abiotic stresses that help them adjust and survive. Some of these changes may even be passed on to progeny and eventually lead to adaptive evolution. Transgenerational changes in response to stress include alterations in DNA methylation and changes in homologous recombination frequency (HRF). The progeny of plants that were stressed often show elevated HRF as well as genomic hypermethylation, although specific loci that are beneficial in times of stress may be hypomethylated. One of the possible mechanisms responsible for passing the memory to the progeny involves small interfering RNAs; Dicer-like proteins, DCL2 and DCL3, are in part required for this process. However, while epigenetic modifications are often present in the untreated progeny of stressed plants, they are not usually sustained for multiple unexposed generations. Still, transgenerational inheritance of such changes has already begun to provide evidence for an important role of epigenetics in enhancing stress resistance.

  20. Evaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis.

    Science.gov (United States)

    Jiang, Chuanhao; Zhao, Feijun; Xiao, Jinhong; Zeng, Tiebing; Yu, Jian; Ma, Xiaohua; Wu, Haiying; Wu, Yimou

    2013-10-01

    Syphilis is a chronic infection caused by Treponema pallidum subsp. pallidum, and diagnosis with sensitive and specific methods is a challenging process that is important for its prevention and treatment. In the present study, we established a recombinant protein TpF1-based indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and a Western blot assay for human and rabbit sera. The 20-kDa recombinant protein TpF1 was detected by Western blotting performed with sera from rabbits immunized with recombinant TpF1 and infected with the T. pallidum Nichols strain and T. pallidum clinical isolates but was not detected by Western blotting with sera from uninfected rabbits. The sensitivity of the recombinant protein was determined by screening sera from individuals with primary, secondary, latent, and congenital syphilis (n = 82). The specificity of the recombinant protein was determined by screening sera from uninfected controls (n = 30) and individuals with potentially cross-reactive infections, including Lyme disease (n = 30) and leptospirosis (n = 5). The sensitivities of TpF1-based ELISAs were 93.3%, 100%, 100%, and 100% for primary, secondary, latent, and congenital syphilis, respectively, and the specificities were all 100% for sera from uninfected controls and individuals with potentially cross-reactive infections. In Western blot assays, the sensitivities and specificities of TpF1 for human sera were all 100%. The reactivities of TpF1 with syphilitic sera were proportional to the titers of the T. pallidum particle agglutination (TPPA) assay. These data indicate that the recombinant protein TpF1 is a highly immunogenic protein in human and rabbit infections and a promising marker for the screening of syphilis.

  1. Cooperative activation of tissue-specific genes by pRB and E2F1.

    Science.gov (United States)

    Flowers, Stephen; Xu, Fuhua; Moran, Elizabeth

    2013-04-01

    The retinoblastoma tumor suppressor protein pRB is conventionally regarded as an inhibitor of the E2F family of transcription factors. Conversely, pRB is also recognized as an activator of tissue-specific gene expression along various lineages including osteoblastogenesis. During osteoblast differentiation, pRB directly targets Alpl and Bglap, which encode the major markers of osteogenesis alkaline phosphatase and osteocalcin. Surprisingly, p130 and repressor E2Fs were recently found to cooccupy and repress Alpl and Bglap in proliferating osteoblast precursors before differentiation. This raises the further question of whether these genes convert to E2F activation targets when differentiation begins, which would constitute a remarkable situation wherein pRB and E2F would be cotargeting genes for activation. Chromatin immunoprecipitation analysis in an osteoblast differentiation model shows that Alpl and Bglap are indeed targeted by an activator E2F, i.e., is E2F1. Promoter occupation of Alpl and Bglap by E2F1 occurs specifically during activation, and depletion of E2F1 severely impairs their induction. Mechanistically, promoter occupation by E2F1 and pRB is mutually dependent, and without this cooperative effect, activation steps previously shown to be dependent on pRB, including recruitment of RNA polymerase II, are impaired. Myocyte- and adipocyte-specific genes are also cotargeted by E2F1 and pRB during differentiation along their respective lineages. The finding that pRB and E2F1 cooperate to activate expression of tissue-specific genes is a paradigm distinct from the classical concept of pRB as an inhibitor of E2F1, but is consistent with the observed roles of these proteins in physiological models.

  2. Scavenger receptors mediate the role of SUMO and Ftz-f1 in Drosophila steroidogenesis.

    Directory of Open Access Journals (Sweden)

    Ana Talamillo

    2013-04-01

    Full Text Available SUMOylation participates in ecdysteroid biosynthesis at the onset of metamorphosis in Drosophila melanogaster. Silencing the Drosophila SUMO homologue smt3 in the prothoracic gland leads to reduced lipid content, low ecdysone titers, and a block in the larval-pupal transition. Here we show that the SR-BI family of Scavenger Receptors mediates SUMO functions. Reduced levels of Snmp1 compromise lipid uptake in the prothoracic gland. In addition, overexpression of Snmp1 is able to recover lipid droplet levels in the smt3 knockdown prothoracic gland cells. Snmp1 expression depends on Ftz-f1 (an NR5A-type orphan nuclear receptor, the expression of which, in turn, depends on SUMO. Furthermore, we show by in vitro and in vivo experiments that Ftz-f1 is SUMOylated. RNAi-mediated knockdown of ftz-f1 phenocopies that of smt3 at the larval to pupal transition, thus Ftz-f1 is an interesting candidate to mediate some of the functions of SUMO at the onset of metamorphosis. Additionally, we demonstrate that the role of SUMOylation, Ftz-f1, and the Scavenger Receptors in lipid capture and mobilization is conserved in other steroidogenic tissues such as the follicle cells of the ovary. smt3 knockdown, as well as ftz-f1 or Scavenger knockdown, depleted the lipid content of the follicle cells, which could be rescued by Snmp1 overexpression. Therefore, our data provide new insights into the regulation of metamorphosis via lipid homeostasis, showing that Drosophila Smt3, Ftz-f1, and SR-BIs are part of a general mechanism for uptake of lipids such as cholesterol, required during development in steroidogenic tissues.

  3. Effects of depleted uranium on the reproductive success and F1 generation survival of zebrafish (Danio rerio)

    Energy Technology Data Exchange (ETDEWEB)

    Bourrachot, Stéphanie [Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France); Brion, François [Institut National de l’Environnement Industriel et des Risques (INERIS), Unité d’évaluation des risques écotoxicologiques, BP2, 60550 Verneuil-en-Halatte (France); Pereira, Sandrine; Floriani, Magali; Camilleri, Virginie; Cavalié, Isabelle [Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France); Palluel, Olivier [Institut National de l’Environnement Industriel et des Risques (INERIS), Unité d’évaluation des risques écotoxicologiques, BP2, 60550 Verneuil-en-Halatte (France); Adam-Guillermin, Christelle, E-mail: christelle.adam-guillermin@irsn.fr [Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, Saint-Paul-lez-Durance 13115 (France)

    2014-09-15

    Highlights: • The effect of depleted uranium on zebrafish reproduction was studied. • An inhibition of egg production and an increase of F1 embryo mortality were observed. • Decreased circulating concentration of vitellogenin was observed in females. • Increased DNA damages were observed in parent gonads and in embryos. • U environmental concentration impairs reproduction and genetic integrity of fish. - Abstract: Despite the well-characterized occurrence of uranium (U) in the aquatic environment, very little is known about the chronic exposure of fish to low levels of U and its potential effect on reproduction. Therefore, this study was undertaken to investigate the effects of environmental concentrations of depleted U on the reproductive output of zebrafish (Danio rerio) and on survival and development of the F1 embryo-larvae following parental exposure to U. For that purpose, sexually mature male and female zebrafish were exposed to 20 and 250 μg/L of U for 14 days and allowed to reproduce in clean water during a further 14-day period. At all sampling times, whole-body vitellogenin concentrations and gonad histology were analyzed to investigate the effects of U exposure on these reproductive endpoints. In addition, accumulation of U in the gonads and its genotoxic effect on male and female gonad cells were quantified. The results showed that U strongly affected the capability of fish to reproduce and to generate viable individuals as evidenced by the inhibition of egg production and the increased rate of mortality of the F1 embryos. Interestingly, U exposure resulted in decreased circulating concentrations of vitellogenin in females. Increased concentrations of U were observed in gonads and eggs, which were most likely responsible for the genotoxic effects seen in fish gonads and in embryos exposed maternally to U. Altogether, these findings highlight the negative effect of environmentally relevant concentrations of U which alter the reproductive

  4. The Influence of Radon (Gas and Progeny) and Weather Conditions on Ambient Dose Equivalent Rate.

    Science.gov (United States)

    Márquez, J L; Benito, G; Saez, J C; Navarro, N; Alvarez, A; Quiñones, J

    2016-08-13

    The purpose of this study is to identify the influence of radon (gas and progeny) on the ambient dose equivalent rate measured at the reference station ESMERALDA, where continuous measurements of the ambient dose equivalent rate (every 10 min) combined with activity concentration measurements of radon gas and radon progeny as well as meteorological parameters have been collected. This study has been performed using a correlation study based on a principal components analysis and the Spearman's rank correlation coefficient.

  5. The hESC line Envy expresses high levels of GFP in all differentiated progeny.

    Science.gov (United States)

    Costa, Magdaline; Dottori, Mirella; Ng, Elizabeth; Hawes, Susan M; Sourris, Koula; Jamshidi, Pegah; Pera, Martin F; Elefanty, Andrew G; Stanley, Edouard G

    2005-04-01

    Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and their differentiated progeny readily in transplantation experiments will facilitate the analysis of hESC potential and function in vivo. We have generated a hESC line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny.

  6. High Correlated Paternity Leads to Negative Effects on Progeny Performance in Two Mediterranean Shrub Species

    Science.gov (United States)

    Nora, Sofia; Aparicio, Abelardo; Albaladejo, Rafael G.

    2016-01-01

    Anthropogenic habitat deterioration can promote changes in plant mating systems that subsequently may affect progeny performance, thereby conditioning plant recruitment for the next generation. However, very few studies yet tested mating system parameters other than outcrossing rates; and the direct effects of the genetic diversity of the pollen received by maternal plants (i.e. correlated paternity) has often been overlooked. In this study, we investigated the relation between correlated paternity and progeny performance in two common Mediterranean shrubs, Myrtus communis and Pistacia lentiscus. To do so, we collected open-pollinated progeny from selected maternal plants, calculated mating system parameters using microsatellite genotyping and conducted sowing experiments under greenhouse and field conditions. Our results showed that some progeny fitness components were negatively affected by the high correlated paternity of maternal plants. In Myrtus communis, high correlated paternity had a negative effect on the proportion and timing of seedling emergence in the natural field conditions and in the greenhouse sowing experiment, respectively. In Pistacia lentiscus, seedling emergence time under field conditions was also negatively influenced by high correlated paternity and a progeny survival analysis in the field experiment showed greater mortality of seedlings from maternal plants with high correlated paternity. Overall, we found effects of correlated paternity on the progeny performance of Myrtus communis, a self-compatible species. Further, we also detected effects of correlated paternity on the progeny emergence time and survival in Pistacia lentiscus, an obligate outcrossed species. This study represents one of the few existing empirical examples which highlight the influence that correlated paternity may exert on progeny performance in multiple stages during early seedling growth. PMID:27835658

  7. Nickel sulfate induces numerous defects in Caenorhabditis elegans that can also be transferred to progeny

    Energy Technology Data Exchange (ETDEWEB)

    Wang Dayong [Department of Genetics and Developmental Biology, Southeast University, Nanjing 210009 (China); Key Laboratory of Developmental Genes and Human Disease, Ministry of Education (China)], E-mail: dayongw@seu.edu.cn; Wang Yang [Department of Genetics and Developmental Biology, Southeast University, Nanjing 210009 (China); Key Laboratory of Developmental Genes and Human Disease, Ministry of Education (China)

    2008-02-15

    Whether the multiple biological toxicities from nickel exposure could be transferred to progeny has not been clarified. In this report, we explored the Caenorhabditis elegans to analyze the multiple toxicities of nickel and their possibly transferable properties. The nickel toxicity caused multiple biological defects in a concentration-dependent manner. Moreover, most of these toxicities could be transferred and could be only partially rescued in progeny. Some specific phenotypes in progeny were also found to exhibit no obvious rescue phenotypes or to show even more severe defects than their parents. The defects caused by nickel exposure could be classified into four groups according to their transferring properties. That is, the defects caused by nickel exposure could be largely, or partially, or unable to be rescued, or became even more severe in progeny animals. Therefore, most of the nickel exposure-caused defects can be transferred from parents to their progeny to different degrees in C. elegans. - Nickel exposure can cause multi-biological toxicities and these defects can be transferred from parents to their progeny in C. elegans.

  8. Meiotic behavior in apomictic Brachiaria ruziziensis × B. brizantha (Poaceae progenies

    Directory of Open Access Journals (Sweden)

    Veridiana Aparecida Fuzinatto

    2012-12-01

    Full Text Available Hybrids combining desirable traits from divergent parents are the main objective of some Brachiaria (Syn. Urochloa P. Beauv. breeding programs. There is great interest in the development of apomictic hybrid cultivars that combine desirable genes such as resistance to spittlebugs, high nutritive value, and tolerance to acid soils. Microsporogenesis of six apomictic progenies resulting from a tetraploid (2n = 4x = 36 cross between B. ruziziensis × B. brizantha was evaluated under light microscopy. Genetic recombination, ensured by multivalent chromosome association and crossing-over at prophase occurred in low frequency among progenies, and in one, recombination was almost nonexistent. The percentage of meiocytes with meiotic abnormalities among progenies ranged from 16.6 % to 85.6 %. Besides an observed irregular chromosome segregation typical of polyploid hybrids in these five progenies, putative meiotic mutations characterized as desynapsis and divergent spindle organization occurred in three progenies. These anomalies caused frequent fractionation of the genome into several microspores of different sizes. In Brachiaria, new cultivars must be apomictic to fix the genotype. However, Brachiaria is a pseudogamous apomict, and viable gametes are necessary to produce viable seeds. Considering meiotic behavior, only two progenies are promising for advancement in the breeding program.

  9. Selection of inbred maize (Zea mays L.) progenies by topcrosses conducted in contrasting environments.

    Science.gov (United States)

    Rodrigues, C S; Pacheco, C A P; Guedes, M L; Pinho, R G V; Castro, C R

    2016-09-23

    The aim of this study was to identify inbred progenies of S0:1 maize (Zea mays L.) plants that were efficient at a low level of technology and responsive at a high level of technology through the use of topcrosses. Two contrasting environments were created using two levels of base fertilization and topdressing, so that the levels of nitrogen, phosphorus, and potassium were applied four times higher in one environment than in the other. We used S0:1 progenies derived from commercial hybrids in topcrosses with two testers (an elite line from the flint heterotic group and an elite line from the dent heterotic group). The progenies and three controls were evaluated in an augmented block design in Nossa Senhora das Dores, SE, Brazil in the 2010 crop season. The average grain yield in the high-technological level was 21.44% greater than that in the low-technological level. There were no changes in progeny behavior in the two technological levels for grain yield. The testers did not differ in the average grain yield of the progenies at the two technological levels. Therefore, it is possible to select progenies derived from commercial hybrids that have an efficient response to fertilization.

  10. Prediction of direct and indirect genetic gains and genotypic correlations in rubber tree progenies

    Directory of Open Access Journals (Sweden)

    Cecília Khusala Verardi

    2011-09-01

    Full Text Available The objective of this work was to estimate the genetic parameters, genotypic and phenotypic correlations, and direct and indirect genetic gains among and within rubber tree (Hevea brasiliensis progenies. The experiment was set up at the Municipality of Jaú, SP, Brazil. A randomized complete block design was used, with 22 treatments (progenies, 6 replicates, and 10 plants per plot at a spacing of 3x3 m. Three‑year‑old progenies were assessed for girth, rubber yield, and bark thickness by direct and indirect gains and genotypic correlations. The number of latex vessel rings showed the best correlations, correlating positively and significantly with girth and bark thickness. Selection gains among progenies were greater than within progeny for all the variables analyzed. Total gains obtained were high, especially for girth increase and rubber yield, which were 93.38 and 105.95%, respectively. Young progeny selection can maximize the expected genetic gains, reducing the rubber tree selection cycle.

  11. M2-F1 on lakebed with pilots Milt Thompson, Chuck Yeager, Don Mallick, and Bruce Peterson

    Science.gov (United States)

    1963-01-01

    -made by Gus Briegleb and company. Ernie Lowder, a NASA craftsman who had worked on the Howard Hughes 'Spruce Goose,' was assigned to help Briegleb. The prototype of a 21st Century spacecraft required the fabrication of hundreds of small wooden parts meticulously nailed and glued together. It was a product of craftsmanship that was nearly obsolete in the 1940s. Final assembly of the remaining components (including aluminum tail surfaces, push rod controls, and landing gear from a Cessna 150) was done back at the NASA facility. In the meantime, other NASA engineers devised a special M2-F1 flight simulator, and a hot rod shop near Long Beach souped-up a Pontiac convertible to be used as the lifting body ground-tow vehicle. The M2-F1 did not have ailerons. Instead, it had elevons which were attached to each of the two rudders. A large flap on the trailing edge of the body acted as an elevator. This unconventional arrangement prompted the engineers to rethink the flight control system as well. They eventually devised two schemes. One system was fairly traditional. It used rudder pedal inputs to move the rudders for yaw control, and stick inputs to provide differential deflections of the elevons for roll. The other system used stick inputs to control the rudders for yaw, while rudder pedal deflections moved the elevons for roll. Milt Thompson tried both systems in the simulator and surprised the design team when he said he preferred system number two. He reasoned that although sideslip delayed roll (which was a result of dihedral effect), the roll rate was twice as high using the rudders instead of the elevons. He said he would rather have the higher roll rates available to him if needed, while the slip could be overcome with proper piloting technique. This was the system that Thompson practiced on the simulator, and he used it during the initial auto tows. Auto tows were done using a 1000 foot rope fastened to the NASA Pontiac. Rogers Dry Lake provided miles of unobstructed

  12. Molecular cloning of chicken FTZ-F1-related orphan receptors.

    Science.gov (United States)

    Kudo, T; Sutou, S

    1997-09-15

    FTZ-F1 is a member of the orphan nuclear receptors, which belongs to the steroid hormone receptor superfamily, and plays a role in the blastoderm and nervous system development in Drosophila. Recently, several FTZ-F1 family genes have been cloned in several species. SF-1/Ad4BPs have been identified as master regulators controlling steroidogenic P-450 genes in mammals and are considered to be the mammalian homologues of FTZ-F1. Moreover, SF-1/Ad4BP plays a critical role in the sexual differentiation of gonads in mammals. In vertebrates, except for mammals, the functional homologue of SF-1/Ad4BP has not been identified before. Herein, we cloned two chicken cDNAs (OR2.0 and OR2.1), which encode putative FTZ-F1 family receptors, by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). OR2.1 consists of 3255 bp, is expressed in the adrenal glands and gonads, and is considered to be the chicken counterpart of mammalian SF-1/Ad4BP. However, OR2.0 consists of 2945 bp, is expressed in the livers and the adrenal glands, and is considered to be the chicken counterpart of mouse LRH-1, which is a member of the FTZ-F1 family in mammals.

  13. Elasticity, friction, and pathway of γ-subunit rotation in FoF1-ATP synthase.

    Science.gov (United States)

    Okazaki, Kei-ichi; Hummer, Gerhard

    2015-08-25

    We combine molecular simulations and mechanical modeling to explore the mechanism of energy conversion in the coupled rotary motors of FoF1-ATP synthase. A torsional viscoelastic model with frictional dissipation quantitatively reproduces the dynamics and energetics seen in atomistic molecular dynamics simulations of torque-driven γ-subunit rotation in the F1-ATPase rotary motor. The torsional elastic coefficients determined from the simulations agree with results from independent single-molecule experiments probing different segments of the γ-subunit, which resolves a long-lasting controversy. At steady rotational speeds of ∼ 1 kHz corresponding to experimental turnover, the calculated frictional dissipation of less than k(B)T per rotation is consistent with the high thermodynamic efficiency of the fully reversible motor. Without load, the maximum rotational speed during transitions between dwells is reached at ∼ 1 MHz. Energetic constraints dictate a unique pathway for the coupled rotations of the Fo and F1 rotary motors in ATP synthase, and explain the need for the finer stepping of the F1 motor in the mammalian system, as seen in recent experiments. Compensating for incommensurate eightfold and threefold rotational symmetries in Fo and F1, respectively, a significant fraction of the external mechanical work is transiently stored as elastic energy in the γ-subunit. The general framework developed here should be applicable to other molecular machines.

  14. Induction of DNA synthesis and apoptosis are separable functions of E2F-1

    DEFF Research Database (Denmark)

    Phillips, A C; Bates, S; Ryan, K M;

    1997-01-01

    The family of E2F transcription factors have an essential role in mediating cell cycle progression, and recently, one of the E2F protein family, E2F-1, has been shown to participate in the induction of apoptosis. Cooperation between E2F and the p53 tumor suppressor protein in this apoptotic...... response had led to the suggestion that cell cycle progression induced by E2F-1 expression provides an apoptotic signal when placed in conflict with an arrest to cell cycle progression, such as provided by p53. We show here that although apoptosis is clearly enhanced by p53, E2F-1 can induce significant...... apoptosis in the absence of p53. Furthermore, this apoptotic function of E2F-1 is separable from the ability to accelerate entry into DNA synthesis. Analysis of E2F-1 mutants indicates that although DNA-binding is required, transcriptional transactivation is not necessary for the induction of apoptosis by E...

  15. Spatial reference memory in normal aging Fischer 344 × Brown Norway F1 hybrid rats.

    Science.gov (United States)

    McQuail, Joseph A; Nicolle, Michelle M

    2015-01-01

    Fischer 344 × Brown Norway F1 (F344 × BN-F1) hybrid rats express greater longevity with improved health relative to aging rodents of other strains; however, few behavioral reports have thoroughly evaluated cognition across the F344 × BN-F1 lifespan. Consequently, this study evaluated spatial reference memory in F344 × BN-F1 rats at 6, 18, 24, or 28 months of age in the Morris water maze. Reference memory decrements were observed between 6 and 18 months and 18 and 24 months. At 28 months, spatial learning was not worse than 24 months, but swim speed was significantly slower. Reliable individual differences revealed that ∼50% of 24- to 28-month-old rats performed similarly to 6 months, whereas others were spatial learning impaired. Aged rats were impaired at learning within daily training sessions but not impaired at retaining information between days of training. Aged rats were also slower to learn to escape onto the platform, regardless of strategy. In summary, these data clarify the trajectory of cognitive decline in aging F344 × BN-F1 rats and elucidate relevant behavioral parameters.

  16. E2F1 in renal cancer: Mr Hyde disguised as Dr Jekyll?

    Science.gov (United States)

    Tian, Weihua; Cui, Fenggong; Esteban, Miguel A

    2013-10-01

    The transcription factor E2F1 has both oncogenic and tumour suppressor properties, depending on the context. Clarifying the function of E2F1 in different types of cancer is relevant because in those situations in which it acts as an oncogene there may be a route for therapeutic interference. Renal cell carcinoma is the most frequent form of kidney cancer in adults and inactivation of the von Hippel-Lindau (VHL) gene underlies most cases. This malignancy represents a challenge for standard therapies due to drug- and radio-resistance, effects that fit well within the scope of functions of E2F1. A new report by Mans et al postulates that up-regulation of E2F1 in VHL-defective renal cell carcinoma induces cell senescence and can thus be considered a good prognostic factor. Here we discuss these findings in a wider context and propose that E2F1 may actually not play a uniform role in renal cell carcinoma but rather an ambiguous one whose deeper understanding could have practical implications. Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

  17. Synergistic cooperation of MDM2 and E2F1 contributes to TAp73 transcriptional activity

    Energy Technology Data Exchange (ETDEWEB)

    Kasim, Vivi, E-mail: vivikasim78@gmail.com [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Huang, Can; Zhang, Jing; Jia, Huizhen; Wang, Yunxia [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Yang, Li [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); The 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Miyagishi, Makoto [Molecular Composite Medicine Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566 (Japan); Wu, Shourong, E-mail: shourongwu@hotmail.com [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); The 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044 (China)

    2014-07-04

    Highlights: • MDM2 is a novel positive regulator of TAp73 transcriptional activity. • MDM2 colocalizes together and physically interacts with E2F1. • Synergistic cooperation of MDM2 and E2F1 is crucial for TAp73 transcription. • MDM2 regulates TAp73 transcriptional activity in a p53-independent manner. - Abstract: TAp73, a structural homologue of p53, plays an important role in tumorigenesis. E2F1 had been reported as a transcriptional regulator of TAp73, however, the detailed mechanism remains to be elucidated. Here we reported that MDM2-silencing reduced the activities of the TAp73 promoters and the endogenous TAp73 expression level significantly; while MDM2 overexpression upregulated them. We further revealed that the regulation of TAp73 transcriptional activity occurs as a synergistic effect of MDM2 and E2F1, most probably through their physical interaction in the nuclei. Furthermore, we also suggested that MDM2 might be involved in DNA damage-induced TAp73 transcriptional activity. Finally, we elucidated that MDM2-silencing reduced the proliferation rate of colon carcinoma cells regardless of the p53 status. Our data show a synergistic effect of MDM2 and E2F1 on TAp73 transcriptional activity, suggesting a novel regulation pathway of TAp73.

  18. The CYP51F1 Gene of Leptographium qinlingensis: Sequence Characteristic, Phylogeny and Transcript Levels

    Directory of Open Access Journals (Sweden)

    Lulu Dai

    2015-05-01

    Full Text Available Leptographium qinlingensis is a fungal associate of the Chinese white pine beetle (Dendroctonus armandi and a pathogen of the Chinese white pine (Pinus armandi that must overcome the terpenoid oleoresin defenses of host trees. L. qinlingensis responds to monoterpene flow with abundant mechanisms that include export and the use of these compounds as a carbon source. As one of the fungal cytochrome P450 proteins (CYPs, which play important roles in general metabolism, CYP51 (lanosterol 14-α demethylase can catalyze the biosynthesis of ergosterol and is a target for antifungal drug. We have identified an L. qinlingensis CYP51F1 gene, and the phylogenetic analysis shows the highest homology with the 14-α-demethylase sequence from Grosmannia clavigera (a fungal associate of Dendroctonus ponderosae. The transcription level of CYP51F1 following treatment with terpenes and pine phloem extracts was upregulated, while using monoterpenes as the only carbon source led to the downregulation of CYP5F1 expression. The homology modeling structure of CYP51F1 is similar to the structure of the lanosterol 14-α demethylase protein of Saccharomyces cerevisiae YJM789, which has an N-terminal membrane helix 1 (MH1 and transmembrane helix 1 (TMH1. The minimal inhibitory concentrations (MIC of terpenoid and azole fungicides (itraconazole (ITC and the docking of terpenoid molecules, lanosterol and ITC in the protein structure suggested that CYP51F1 may be inhibited by terpenoid molecules by competitive binding with azole fungicides.

  19. Suppression of fabB Mutation by fabF1 Is Mediated by Transcription Read-through in Shewanella oneidensis.

    Science.gov (United States)

    Li, Meng; Meng, Qiu; Fu, Huihui; Luo, Qixia; Gao, Haichun

    2016-11-15

    As type II fatty acid synthesis is essential for the growth of Escherichia coli, its many components are regarded as potential targets for novel antibacterial drugs. Among them, β-ketoacyl-acyl carrier protein (ACP) synthase (KAS) FabB is the exclusive factor for elongation of the cis-3-decenoyl-ACP (cis-3-C10-ACP). In our previous study, we presented evidence to suggest that this may not be the case in Shewanella oneidensis, an emerging model gammaproteobacterium renowned for its respiratory versatility. Here, we identified FabF1, another KAS, as a functional replacement for FabB in S. oneidensis In fabB(+) or desA(+) (encoding a desaturase) cells, which are capable of making unsaturated fatty acids (UFA), FabF1 is barely produced. However, UFA auxotroph mutants devoid of both fabB and desA genes can be spontaneously converted to suppressor strains, which no longer require exogenous UFAs for growth. Suppression is caused by a TGTTTT deletion in the region upstream of the fabF1 gene, resulting in enhanced FabF1 production. We further demonstrated that the deletion leads to transcription read-through of the terminator for acpP, an acyl carrier protein gene immediately upstream of fabF1 There are multiple tandem repeats in the region covering the terminator, and the TGTTTT deletion, as well as others, compromises the terminator efficacy. In addition, FabF2 also shows an ability to complement the FabB loss, albeit substantially less effectively than FabF1.

  20. 26 CFR 1.167(f)-1 - Reduction of salvage value taken into account for certain personal property.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Reduction of salvage value taken into account for certain personal property. 1.167(f)-1 Section 1.167(f)-1 Internal Revenue INTERNAL REVENUE SERVICE... for Individuals and Corporations § 1.167(f)-1 Reduction of salvage value taken into account for...

  1. 40 CFR Table F-1 to Subpart F of... - Performance Specifications for PM2.5 Class II Equivalent Samplers

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 5 2010-07-01 2010-07-01 false Performance Specifications for PM2.5 Class II Equivalent Samplers F Table F-1 to Subpart F of Part 53 Protection of Environment ENVIRONMENTAL..., Subpt. F, Table F-1 Table F-1 to Subpart F of Part 53—Performance Specifications for PM2.5 Class...

  2. Conditional E2F1 activation in transgenic mice causes testicular atrophy and dysplasia mimicking human CIS

    DEFF Research Database (Denmark)

    Agger, Karl; Santoni-Rugiu, Eric; Holmberg, Christian;

    2005-01-01

    E2F1 is a crucial downstream effector of the retinoblastoma protein (pRB) pathway. To address the consequences of short-term increase in E2F1 activity in adult tissues, we generated transgenic mice expressing the human E2F1 protein fused to the oestrogen receptor (ER) ligand-binding domain...

  3. File list: Oth.ALL.50.Pou2f1.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.50.Pou2f1.AllCell mm9 TFs and others Pou2f1 All cell types SRX877595,SRX877...592 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.50.Pou2f1.AllCell.bed ...

  4. MEASUREMENT OF RADON, THORON AND THEIR PROGENY IN DIFFERENT TYPES OF DWELLING IN ALMORA DISTRICT OF KUMAUN HIMALAYAN REGION.

    Science.gov (United States)

    Singh, Kuldeep; Semwal, Poonam; Pant, Preeti; Gusain, G S; Joshi, Manish; Sapra, B K; Ramola, R C

    2016-10-01

    The indoor concentrations of radon ((222)Rn), thoron ((220)Rn) and their daughter products were measured in the dwellings of Almora district in Kumaun Himalaya, India using pin-hole dosemeters and deposition progeny sensors. The measurements were made in the residential houses built of mud, stone with cement plaster and cemented house during winter season. Average [geometric mean (GM) values] radon and thoron concentrations for all dwellings were found to be 99.82 and 79.70 Bq m(-3), respectively, while average equilibrium equivalent radon concentration and equilibrium equivalent thoron concentration (measured for the first time for this region) were measured at 35.22 and 2.52 Bq m(-3), respectively. Radon concentration (GM values) was found to be 110.73, 97.00 and 93.85 Bq m(-3) for mud houses, stone with cemented plaster houses and cemented houses, respectively. On the other hand, thoron concentration values were 87.10, 75.79 and 75.68 Bq m(-3) for cemented houses, mud houses and stone with cemented plaster houses, respectively. Interpretations have been made on the basis of measured radon/thoron and progeny concentration values with respect to the difference of construction material of the dwellings. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. Decreased dietary protein or energy intake and plasma growth hormone levels of the pregnant pig, its fetuses and developing progeny.

    Science.gov (United States)

    Atinmo, T; Baldijrao, C; Pond, W G; Barnes, R H

    1976-07-01

    The effects of low protein diets on plasma growth hormone were studied in pregnant pigs, fetuses and the developing progeny. Pregnant pigs were fed 18%, 3% or 0.5% protein diet throughout the gestation period. At 10, 13 and 15 week of gestation, fetuses were removed from the uterus after the dam had been bled to death. Plasma samples were used for growth hormone determinations. In a second experiment, 2-day old pigs from another set of pregnant pigs fed the diet containing 18%, 3% or 0.5% protein during gestation were cross-fostered to control nursing dams and weaned at 4 weeks of age to a standard diet. Plasma obtained at regular intervals was used for growth hormone determination. Plasma growth hormone was significantly higher in dams fed 0.5% protein after week 13 of gestation. High growth hormone (ten times the dam GH level) was observed in all fetuses irrespective of maternal dietary manipulation. Offspring of severely protein deprived pits (0.5% protein) had significantly elevated growth hormone levels up to 12 weeks of age in spite of cross fostering to a control dam after birth. The data suggest that there is little or no effect of maternal protein restriction on fetal growth hormone levels but the persistent high growth hormone levels in the progeny of severely malnourished pigs indicate a possible impairment of the production, release or catabolism of growth hormone and/or its releasing factor.

  6. Search for major genes with progeny test data to accelerate the development of genetically superior loblolly pine

    Energy Technology Data Exchange (ETDEWEB)

    NCSU

    2003-12-30

    This research project is to develop a novel approach that fully utilized the current breeding materials and genetic test information available from the NCSU-Industry Cooperative Tree Improvement Program to identify major genes that are segregating for growth and disease resistance in loblolly pine. If major genes can be identified in the existing breeding population, they can be utilized directly in the conventional loblolly pine breeding program. With the putative genotypes of parents identified, tree breeders can make effective decisions on management of breeding populations and operational deployment of genetically superior trees. Forest productivity will be significantly enhanced if genetically superior genotypes with major genes for economically important traits could be deployed in an operational plantation program. The overall objective of the project is to develop genetic model and analytical methods for major gene detection with progeny test data and accelerate the development of genetically superior loblolly pine. Specifically, there are three main tasks: (1) Develop genetic models for major gene detection and implement statistical methods and develop computer software for screening progeny test data; (2) Confirm major gene segregation with molecular markers; and (3) Develop strategies for using major genes for tree breeding.

  7. BMW Sauber F1 Team 车队专用——BMW X PUMA

    Institute of Scientific and Technical Information of China (English)

    Brian

    2007-01-01

    Puma x F1.第一时间会想起Puma与法拉利车队合作的法拉利系列.还有,Puma更与法拉利签下长期合作协议。可是.最近却杀出个程咬金-BMW Sauber F1 Team车队,说的是Puma与宝马车队联合设计的新系.当中最注目的有这双Kart Cat Ⅱ F1赛车鞋.鞋形延用Puma Cat一贯的流线形设计,除了用上宝马车队专用的宝蓝色外.中底更用上Puma CeⅡ蜂巢缓震系统,算是向法拉利车队示威吧!

  8. Optical pumping effect in absorption imaging of F =1 atomic gases

    Science.gov (United States)

    Kim, Sooshin; Seo, Sang Won; Noh, Heung-Ryoul; Shin, Y.

    2016-08-01

    We report our study of the optical pumping effect in absorption imaging of 23Na atoms in the F =1 hyperfine spin states. Solving a set of rate equations for the spin populations in the presence of a probe beam, we obtain an analytic expression for the optical signal of the F =1 absorption imaging. Furthermore, we verify the result by measuring the absorption spectra of 23Na Bose-Einstein condensates prepared in various spin states with different probe-beam pulse durations. The analytic result can be used in the quantitative analysis of F =1 spinor condensate imaging and readily applied to other alkali-metal atoms with I =3 /2 nuclear spin such as 87Rb.

  9. Types of tree growth and fruit setting in F1 apple hybrids

    Directory of Open Access Journals (Sweden)

    Radu E. SESTRAS

    1998-08-01

    Full Text Available 1656 F1 hybrid apple seedlings, belonging to 127 combinations, have been screened according to their growing and fruit setting types, as it was phenotypically expressed. LESPINASSE (1977; 1992 amalgamated these two traits into a single one which was named ";ideotype";. The screened F1 individuals have been considered as resembling one of the following four architectural ideotypes of the trees indicated by Lespinasse: columnar, spur, standard and weeping. Different ratios of spur, standard and columnar F1 individuals were obtained depending on genitors and on the fact that a certain genitor had been used as a maternal or paternal partner in direct/reciprocal crosses. The monogenic inheritance of the columnar ideotype, proposed by Kelsey and Brown (1992; Lane (1992, does not seem to be the only genetic mechanism involved in the inheritance of this trait. Our experimental results suggest the polygenic determination of this ideotype as more probable than the monogenic one.

  10. 墨西哥考虑2010年重返F1

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    F1最早一次造访墨西哥是1962年,中间反复了两次,最后一次承办是1992年。墨西哥此后一直嚷嚷要重新承办F1,但这次是非常认真的,计划在2010年重返F1赛历。据来自墨西哥的消息称,有很多地方考虑新建赛道,其中呼声最高的是位于尤卡坦半岛的坎昆,另外两个地点是Puebla和Tijuana。

  11. Studies on the electrical characteristics of thoron ({sup 220}Rn) progeny

    Energy Technology Data Exchange (ETDEWEB)

    Maniyan, C.G.; Louiz, J.; Pillai, P.M.B.; Khan, A.H

    2003-07-01

    Electrical characteristics of thoron ({sup 220}Rn) progeny under various low ampere (<1 mA) DC voltages applied to a calibration facility (CF) has been studied. About 30 y old thorium hydroxide was used as the source for the generation of thoron and its decay products. The study has revealed that under a low ventilation rate (about 1 air change per hour) and at high equilibrium equivalent concentration (EER) of thoron, above 275 Bq m{sup -3} (>1 WL), a considerable fraction of {sup 212}Pb nuclides are negatively charged. It was also concluded that {sup 212}Pb has lesser charge (per ion) than {sup 212}Bi and is more susceptible to an electric field. There is a linear relationship between the activity collected and the voltage applied and the activity collected is proportional to the area of the collecting surface. Under the experimental conditions, when a voltage of {+-}2500 V is simultaneously applied, the activity concentration can be reduced by a factor of 100. (author)

  12. Norethindrone-induced masculinization and progeny testing in guppy, Poecilia reticulata (Peters 1859).

    Science.gov (United States)

    Basavaraja, N; Chandrashekhara, B H; Ahamad, Rather Mansoor

    2014-03-01

    Norethindrone(NE) was evaluated for its efficacy on alteration of sex ratio of P. reticulata. Either the young fry or the brooders and the resultant fry were fed a commercial diet incorporated with NE at 0, 25, 50, 75 and 100 mg kg(-1) diet (ppm) for 30-40 d in rectangular glass aquaria; this was followed by 40-60 d rearing on NE-free diet in out-door concrete tanks. In general, the androgen treatment altered sex ratio, leading to the production of a dose dependent increase in the percentage of males. The oral administration of the steroid at 75 ppm for 40 d or 100 ppmfor 30 or 40 d to first feeding fry, yielded 100% males. On the other hand, NE administration to brooders before parturition and the resultant fry also produced an all-male population of guppy. The sex ratio of the untreated control was almost 1:1. The survival of fish in all the trials was high, ranging between 67 and 100%. Mating masculinized males ("XX" male) with normal female resulted in an all-female progeny, while crossing normal male (XY) from treatment groups with normal female sired normal sex ratio (1:1), elucidating XX-XY sex determination system in the guppy.

  13. Cat (Fel d 1) and dog (Can f 1) allergen levels in cars, dwellings and schools.

    Science.gov (United States)

    Niesler, A; Ścigała, G; Łudzeń-Izbińska, B

    Pets are an important source of indoor allergens. The aim of the study was to compare cat and dog allergen levels in cars, schools and homes. The study was carried out in 17 cars, 14 classrooms and 19 dwellings located in the highly industrialized and urbanized region of Poland. Dust and air samples were analyzed for Fel d 1 and Can f 1 using a double monoclonal ELISA assay. The highest amounts of cat and dog allergens (Fel d 1: 1169 μg/g; Can f 1: 277 μg/g) were found in dwellings with pets. Allergen concentrations were correlated with the number of animals kept at home. Although concentrations on automobile seats were lower, Fel d 1 levels exceeded 8 μg/g in 23.5 % of cars and high levels of Can f 1 (>10 μg/g) were found in 17.6 % of cars. The study revealed that cars of pet owners may be reservoirs of cat and dog allergens even when animals are not transported in them. In schools, concentrations of pet allergens did not reach high levels, but the moderate levels of Fel d 1 (≥1-8 μg/g) and Can f 1 (≥2-10 μg/g) were detected in 42.9 and 7.1 % of the investigated classrooms. Concentrations of cat and dog allergen in schools were higher than in homes without pets. While airborne Fel d 1 and Can f 1 levels were found low, residential allergen concentrations in settled dust and air were correlated. The study results suggest that classrooms and cars of pet owners may be important sites of exposure to cat and dog allergens, though the highest concentrations of Fel d 1 and Can f 1 are found in homes of pet owners.

  14. 番茄新品种浙杂806的选育%A New Tomato F1 Hybrid — ‘Zheza 806’

    Institute of Scientific and Technical Information of China (English)

    杨悦俭; 马忠翼; 周国智

    2001-01-01

    Zheza 806 is a new indeterminate F1 hybrid. Its female parent line is T217 that selected from the progenies of the cross between processing variety Zhehong 20 and Manapal Tm-2nv introduced from US. T217 has the gene to resist TMV and double recessive characteristics — yellowish leaf and green stem for easy to distinguish false F1 seedlings. Male parent,87-122-4,is selected from Xiaojixin,a Shanghai local determinate variety. This hybrid F1 suits plastic house cultivation in winter and early spring season,as well as open field in spring and highland in summer. It has very good quality fruit with red color and high-resistance to TMV and mid-resistance to CMV. Its average yield is about 75 t*hm-2. The total plant acreage is around 1 000 hm2 in Zhejiang and some area nearby.%浙杂806的母本为品质极佳的加工品种浙红20和美国引进材料Manapal Tm-2nv的杂交后代经多代株系选育而成的T217,具有高抗TMV的Tm-2nv基因和黄绿叶、绿茎双隐性标记性状,可方便剔除假杂种。父本87-122-4是从上海地方品种小鸡心中系选而成。该杂种一代为无限生长类型,适合长江流域冬春大棚栽培,尤适春季露地和夏秋高山栽培,高抗TMV、中抗CMV,单果质量220 g,果实大红色、高圆形,可溶性固形物4.6 %,品质风味佳,每667 m2产量在5 000 kg左右,高产可达7 000 kg以上。在浙江、福建等地已推广1000 hm2。

  15. Operating principles of rotary molecular motors: differences between F1 and V1 motors.

    Science.gov (United States)

    Yamato, Ichiro; Kakinuma, Yoshimi; Murata, Takeshi

    2016-01-01

    Among the many types of bioenergy-transducing machineries, F- and V-ATPases are unique bio- and nano-molecular rotary motors. The rotational catalysis of F1-ATPase has been investigated in detail, and molecular mechanisms have been proposed based on the crystal structures of the complex and on extensive single-molecule rotational observations. Recently, we obtained crystal structures of bacterial V1-ATPase (A3B3 and A3B3DF complexes) in the presence and absence of nucleotides. Based on these new structures, we present a novel model for the rotational catalysis mechanism of V1-ATPase, which is different from that of F1-ATPases.

  16. An approximation for zero-balanced Appell function $F_1$ near $(1,1)$

    OpenAIRE

    Karp, D.

    2007-01-01

    We suggest an approximation for the zero-balanced Appell hypergeometric function $F_1$ near the singular point $(1,1)$. Our approximation can be viewed as a generalization of Ramanujan's approximation for zero-balanced ${_2F_1}$ and is expressed in terms of ${_3F_2}$. We find an error bound and prove some basic properties of the suggested approximation which reproduce the similar properties of the Appell function. Our approximation reduces to the approximation of Carlson-Gustafson when the Ap...

  17. HMGA2 induces pituitary tumorigenesis by enhancing E2F1 activity

    DEFF Research Database (Denmark)

    Fedele, Monica; Visone, Rosa; De Martino, Ivana

    2006-01-01

    HMGA2 gene amplification and overexpression in human prolactinomas and the development of pituitary adenomas in HMGA2 transgenic mice showed that HMGA2 plays a crucial role in pituitary tumorigenesis. We have explored the pRB/E2F1 pathway to investigate the mechanism by which HMGA2 acts. Here we......2 mice. Thus, HMGA2-mediated E2F1 activation is a crucial event in the onset of these tumors in transgenic mice and probably also in human prolactinomas....

  18. Engineering a light-controlled F1 ATPase using structure-based protein design

    OpenAIRE

    2016-01-01

    The F1 sub-complex of ATP synthase is a biological nanomotor that converts the free energy of ATP hydrolysis into mechanical work with an astonishing efficiency of up to 100% (Kinosita et al., 2000). To probe the principal mechanics of the machine, I re-engineered the active site of E.coli F1 ATPase with a structure-based protein design approach: by incorporation of a site-specific, photoswitchable crosslinker, whose end-to-end distance can be modulated by illumination with light of two diffe...

  19. In vivo and ex vivo phagocytic potential of macrophages from progeny of breeder hens kept on ochratoxin A (OTA)-contaminated diet.

    Science.gov (United States)

    Zahoor-ul-Hassan; Khan, Muhammad Zargham; Khan, Ahrar; Javed, Ijaz; Noreen, Mnaza

    2012-01-01

    This study aimed to investigate the phagocytic potential of macrophages in progeny of breeder hens kept on an OTA-contaminated diet. For this purpose, 84 White Leghorn (WL) layer breeder hens (40-weeks-of-age) were divided into seven groups (A-G). Hens in Group A were fed a commercial layer ration while those in Groups B-G were kept on a diet amended with 0.1, 0.5, 1.0, 3.0, 5.0, or 10.0 mg OTA/kg, respectively, for up to 3 weeks (n = 12/treatment group; n = 4/time sub-group/treatment group). Fertile eggs were set for hatching on a weekly basis to get the progeny of each week separately. Hatched chicks (n = 10 from each group) were injected with India ink at day 14-of-age to study the in vivo phagocytosis of carbon particles. At day 30, abdominal macrophages were collected from 15 chicks/group and were used to assess their ex vivo/in vitro phagocytic potential against sheep red blood cells (SRBC) as well as for nitrite production upon challenge with lipopolysaccharide (LPS). The phagocytic indices of the reticuloendothelial system of all three sets of progeny (chicks obtained from hens fed OTA for 7, 14, and 21 days) were significantly lower than values seen with Group A chicks. The number of macrophages that were actively phagocytic, the number of SRBC internalized per macrophage, and the extent of nitrite production after stimulation with LPS were each significantly lower in the cells obtained from chicks of breeder hens that had been maintained on the OTA-contaminated diets. The findings of this study clearly showed that there are immunosuppressive effects-in terms of depressed in vivo and in vitro macrophage functionality-in progeny of OTA-fed breeder hens.

  20. An optical sensor for hydrogen sulfide detection in open path using WMS-2 f/1 f technique

    Science.gov (United States)

    Song, Li-mei; Liu, Li-wen; Yang, Yan-gang; Guo, Qing-hua; Xi, Jiang-tao

    2016-11-01

    An optical hydrogen sulfide (H2S) sensor based on wavelength modulation spectroscopy with the second harmonic (2 f) corrected by the first harmonic (1 f) signal (WMS-2 f/1 f) is developed using a distributed feedback (DFB) laser emitting at 1.578 μm and a homemade gas cell with 1-m-long optical path length. The novel sensor is constructed by an electrical cabinet and an optical reflecting and receiving end. The DFB laser is employed for targeting a strong H2S line at 6 336.62 cm-1 in the fundamental absorption band of H2S. The sensor performance, including the minimum detection limit and the stability, can be improved by reducing the laser intensity drift and common mode noise by means of the WMS-2 f/1 f technique. The experimental results indicate that the linearity and response time of the sensor are 0.999 26 and 6 s (in concentration range of 15.2—45.6 mg/m3), respectively. The maximum relative deviation for continuous detection (60 min) of 30.4 mg/m3 H2S is 0.48% and the minimum detection limit obtained by Allan variance is 79 μg/m3 with optimal integration time of 32 s. The optical H2S sensor can be applied to environmental monitoring and industrial production, and it has significance for real-time online detection in many fields.

  1. Chromosomal Behavior during Meiosis in the Progeny of Triticum timopheevii × Hexaploid Wild Oat.

    Directory of Open Access Journals (Sweden)

    Hongzhou An

    Full Text Available The meiotic behavior of pollen mother cells (PMCs of the F2 and F3 progeny from Triticum timopheevii × hexaploid wild oat was investigated by cytological analysis and sequential C-banding-genomic in situ hybridization (GISH in the present study. A cytological analysis showed that the chromosome numbers of the F2 and F3 progeny ranged from 28 to 41. A large number of univalents, lagging chromosomes, chromosome bridges and micronuclei were found at the metaphase I, anaphase I, anaphase II and tetrad stages in the F2 and F3 progeny. The averages of univalents were 3.50 and 2.73 per cell, and those of lagging chromosomes were 3.37 and 1.87 in the F2 and F3 progeny, respectively. The PMC meiotic indices of the F2 and F3 progeny were 12.22 and 20.34, respectively, indicating considerable genetic instability. A sequential C-banding-GISH analysis revealed that some chromosomes and fragments from the hexaploid wild oat were detected at metaphase I and anaphase I in the progeny, showing that the progeny were of true intergeneric hybrid origin. The alien chromosomes 6A, 7A, 3C and 2D were lost during transmission from F2 to F3. In addition, partial T. timopheevii chromosomes appeared in the form of univalents or lagging chromosomes, which might result from large genome differences between the parents, and the wild oat chromosome introgression interfered with the wheat homologues' normally pairing.

  2. Inhalation dose due to radon, thoron, and progenies in dwellings of a hill station.

    Science.gov (United States)

    Sivakumar, R

    2017-02-01

    The general public spends a major portion of their time in an indoor environment and hence receives a considerable amount of radiation. Knowledge about indoor radiation is important in order to arrive at the actual effective dose received by residents. The indoor radon, thoron, and progeny concentrations observed in the present study were found to vary with seasons of a given year. The highest and lowest indoor average radon, thoron, and progeny levels were observed during winter and summer seasons, respectively. The concentrations of indoor radon, thoron, and progenies were found to vary with the type of houses. The highest (222)Rn, (220)Rn, and progeny concentrations were observed in mud houses and the lowest values were recorded in wooden houses. The indoor (222)Rn concentration correlated well with concentration of its grandparent (238)U in underlying soil with a correlation coefficient of 0.87. The correlation between indoor (220)Rn and (232)Th in the underlying soil was found to be 0.64. The estimated effective doses received by the general public in the present study due to indoor radon and thoron were 1.49 ± 0.49 and 1.30 ± 0.53 mSv/year, respectively. The annual effective doses due to radon and thoron progenies were estimated as 0.76 ± 0.27 and 0.47 ± 0.23 mSv/year, respectively. The contributions from (222)Rn, (220)Rn, and corresponding progenies to the annual effective doses received were 37, 32, 19, and 12%, respectively. The general public living in the study area receives an inhalation dose of 4.02 mSv/year due to indoor radon, thoron, and progenies, which were found to be less than the action limit of ICRP 2009.

  3. Improvement in genetic characteristics and oil yield of selected soybean progenies from octuple crosses

    Directory of Open Access Journals (Sweden)

    Hamawaki Osvaldo Toshiyuki

    2000-01-01

    Full Text Available The objective of the present study was to evaluate 44 soybean octuple crosses in the F4:3[8] and F5:3[8] generations in order to select progenies superior for seed oil yield (OY and other important agronomic characteristics. Octuple crosses were hybridized in a chain mating system. In one group, crosses were carried out for three generations with the adapted x exotic parents until octuple crosses with 75% adapted genes and 25% exotic genes were obtained. In a second group, hybridization of adapted x adapted parents originated crosses with 100% adapted genes. During the growing season 1994/95, the progenies F4:3[8] were evaluated by using the augmented block design. The progenies F5:3[8] were evaluated during the growing season 1995/96 in three experiments using augmented block design without repetition. The octuple crosses gave origin to superior progenies for all the characters studied. In the C22 cross, OY values were 707 kg/ha. The estimates of heritability in relation to the crosses average resulted in the following mean, minimum and maximum values, respectively: number of days to maturity (52.35%, 3.71%, 84.23%; agronomic value (26.69%, 1.62%, 61.28% and grain yield (29.28%, 1.52%, 61.06%. The observed genetic gains for grain yield in the early, intermediate and late F5:3[8] progenies were superior to the expected genetic gains and the observed genetic gains for OY were more expressive in the early and late F5:[8] progenies. The genetic variability remaining in the selected progenies of some crosses suggests that further genetic gains for grain yield and OY might be possible with advanced selection cycles.

  4. E2F1-Mediated Induction of NFYB Attenuates Apoptosis via Joint Regulation of a Pro-Survival Transcriptional Program.

    Directory of Open Access Journals (Sweden)

    Xiaolei Jiang

    Full Text Available The E2F1 transcription factor regulates cell proliferation and apoptosis through the control of a considerable variety of target genes. Previous work has detailed the role of other transcription factors in mediating the specificity of E2F function. Here we identify the NF-YB transcription factor as a novel direct E2F1 target. Genome-wide expression analysis of the effects of NFYB knockdown on E2F1-mediated transcription identified a large group of genes that are co-regulated by E2F1 and NFYB. We also provide evidence that knockdown of NFYB enhances E2F1-induced apoptosis, suggesting a pro-survival function of the NFYB/E2F1 joint transcriptional program. Bioinformatic analysis suggests that deregulation of these NFY-dependent E2F1 target genes might play a role in sarcomagenesis as well as drug resistance.

  5. Measurement of growth curve in F1 generation of Rongshui miniature pig%融水小型猪 F1代生长研究

    Institute of Scientific and Technical Information of China (English)

    施赫赫; 陈淦; 刘运忠; 刘科; 邝少松; 任海涛; 余细勇; 唐小江

    2015-01-01

    目的:测定融水小型猪F1代体重和体尺。方法选取F1代融水小型猪83头(雌性48头,雄性35头),测定初生至12月龄的体重、体长、体高、胸围、胸宽、胸深、管围、腿围、嘴裂长度共9个生长发育指标,并应用SPSS统计软件和Logistic非线性生长模型进行分析。结果融水小型猪F1代的初生体重雌雄分别为0.61±0.14 kg和0.55±0.13 kg,6月龄体重雌雄分别为17.21±5.20 kg和16.35±5.23 kg,12月龄体重雌雄分别为26.97±6.49 kg和26.53±5.65 kg。雌雄比较,9项指标所测结果接近,除了初生体重和体长、10月龄胸宽有差异( P <0.05),其余指标同月龄雌雄之间均无明显差异。应用Logistic模型分析,体重生长拐点在5~6月龄间,体长和腿围生长拐点在2~3月龄间,体高、胸围、胸宽、胸深、管围和嘴裂长度的生长拐点在1~2月龄间。结论融水小型猪F1代成年体重轻,性情温顺,具备培养成实验用小型猪基本条件。%Objective To measure the body weight and body size of the F1 generation in Rongshui miniature pig ( RMP ).Methods 83 F1 generations of RMPs (48 females and 35 males) were selected randomly.9 traits included body-weight, body-length, body-height, chest-circumference, chest-breadth, chest-depth, circum of pastern, girth of leg and rictus were measured, and analyzed statistically by SPSS statistical software and Logistic nonlinear growth analysis model.Results In the F1 generations of RMP, the weights of birth day、6thmonth and 12th month of female and male were 0.61 ±0.14 kg and 0.55 ±0.13 kg, 17.21 ±5.20 kg and 16.35 ±5.23 kg, 26.97 ±6.49 kg and 26.53 ±5.65 kg respectively.There was no difference significantly between the genders of the 9 measured traits except for born-weight, born-length and chest-breadth in 10th month ( P <0.05 ).According to the analysis in Logistic model, body-weight inflection point was between 5th -6th month, body length

  6. Structures of the thermophilic F1-ATPase epsilon subunit suggesting ATP-regulated arm motion of its C-terminal domain in F1.

    Science.gov (United States)

    Yagi, Hiromasa; Kajiwara, Nobumoto; Tanaka, Hideaki; Tsukihara, Tomitake; Kato-Yamada, Yasuyuki; Yoshida, Masasuke; Akutsu, Hideo

    2007-07-03

    The epsilon subunit of bacterial and chloroplast F(o)F(1)-ATP synthases modulates their ATP hydrolysis activity. Here, we report the crystal structure of the ATP-bound epsilon subunit from a thermophilic Bacillus PS3 at 1.9-A resolution. The C-terminal two alpha-helices were folded into a hairpin, sitting on the beta sandwich structure, as reported for Escherichia coli. A previously undescribed ATP binding motif, I(L)DXXRA, recognizes ATP together with three arginine and one glutamate residues. The E. coli epsilon subunit binds ATP in a similar manner, as judged on NMR. We also determined solution structures of the C-terminal domain of the PS3 epsilon subunit and relaxation parameters of the whole molecule by NMR. The two helices fold into a hairpin in the presence of ATP but extend in the absence of ATP. The latter structure has more helical regions and is much more flexible than the former. These results suggest that the epsilon C-terminal domain can undergo an arm-like motion in response to an ATP concentration change and thereby contribute to regulation of F(o)F(1)-ATP synthase.

  7. Cleanup Verification Package for the 126-F-1, 184-F Powerhouse Ash Pit

    Energy Technology Data Exchange (ETDEWEB)

    S. W. Clark and H. M. Sulloway

    2007-09-26

    This cleanup verification package documents completion of remedial action for the 126-F-1, 184-F Powerhouse Ash Pit. This waste site received coal ash from the 100-F Area coal-fired steam plant. Leakage of process effluent from the 116-F-14 , 107-F Retention Basins flowed south into the ash pit, contaminating the northern portion.

  8. 76 FR 28997 - Extension of Employment Authorization for Haitian F-1 Nonimmigrant Students Experiencing Severe...

    Science.gov (United States)

    2011-05-19

    ... earthquake. See 75 FR 3476. Haiti has limited resources to cope with a natural disaster like this earthquake... students whose country of citizenship is Haiti and who are experiencing severe economic hardship as a... requirements governing on-campus and off-campus employment for F-1 nonimmigrant students whose country...

  9. F1 rotary motor of ATP synthase is driven by the torsionally-asymmetric drive shaft

    Science.gov (United States)

    Kulish, O.; Wright, A. D.; Terentjev, E. M.

    2016-06-01

    F1F0 ATP synthase (ATPase) either facilitates the synthesis of ATP in a process driven by the proton moving force (pmf), or uses the energy from ATP hydrolysis to pump protons against the concentration gradient across the membrane. ATPase is composed of two rotary motors, F0 and F1, which compete for control of their shared γ -shaft. We present a self-consistent physical model of F1 motor as a simplified two-state Brownian ratchet using the asymmetry of torsional elastic energy of the coiled-coil γ -shaft. This stochastic model unifies the physical concepts of linear and rotary motors, and explains the stepped unidirectional rotary motion. Substituting the model parameters, all independently known from recent experiments, our model quantitatively reproduces the ATPase operation, e.g. the ‘no-load’ angular velocity is ca. 400 rad/s anticlockwise at 4 mM ATP. Increasing the pmf torque exerted by F0 can slow, stop and overcome the torque generated by F1, switching from ATP hydrolysis to synthesis at a very low value of ‘stall torque’. We discuss the motor efficiency, which is very low if calculated from the useful mechanical work it produces - but is quite high when the ‘useful outcome’ is measured in the number of H+ pushed against the chemical gradient.

  10. E. coli F1-ATPase interacts with a membrane protein component of a proton channel.

    Science.gov (United States)

    Walker, J E; Saraste, M; Gay, N J

    1982-08-26

    The ATP synthases of bacteria, mitochondria and chloroplasts, which use the energy of a transmembrane proton gradient to power the synthesis of ATP, consist of an integral membrane component F0--thought to contain a proton channel--and a catalytic component, F1. To help investigate the way F0 and F1 are coupled, we have sequenced the b-subunit of the Escherichia coli F0, which seems to be the counterpart of a thermophilic bacteria F0 subunit thought to be essential for F1 binding. We report here that its sequence is remarkable, being hydrophobic around the N-terminus and highly charged in the remainder. We propose that the N-terminal segment lies in the membrane and the rest outside. The extramembranous section contains two adjacent stretches of 31 amino acids where the sequence is very similar: in the second of these stretches there is further internal homology. These duplicated stretches of the polypeptide probably fold into two alpha-helices which have many common features able to make contact with F1 subunits. Thus protein b occupies a central position in the enzyme, where it may be involved in proton translocation. It is possibly also important in biosynthetic assembly.

  11. Nucleotide occupancy of F1-ATPase catalytic sites under crystallization conditions.

    Science.gov (United States)

    Löbau, S; Weber, J; Senior, A E

    1997-03-03

    Using site-directed tryptophan fluorescence we studied nucleotide occupancy of the catalytic sites of Escherichia coli F1-ATPase, under conditions used previously for crystallization and X-ray structure analysis of the bovine mitochondrial enzyme [Abrahams et al. (1994) Nature 370, 621-628]. We found that only two of the three catalytic sites were filled in the E. coli enzyme under these conditions (250 microM MgAMPPNP plus 5 microM MgADP), consistent with what was reported in the bovine F1 X-ray structure. However, subsequent addition of a physiological concentration of MgATP readily filled the third catalytic site. Therefore the enzyme form seen in the X-ray structure results from the fact that it is obtained under sub-saturating nucleotide conditions. The data show that the X-ray structure is compatible with a catalytic mechanism in which all three F1-ATPase catalytic sites must fill with MgATP to initiate steady-state hydrolysis [e.g. Weber and Senior (1996) Biochim. Biophys. Acta 1275, 101-104]. The data further demonstrate that the site-directed tryptophan fluorescence technique can provide valuable support for F1 crystallography studies.

  12. Localization of Magic-F1 Transgene, Involved in Muscular Hypertrophy, during Early Myogenesis

    Science.gov (United States)

    Ronzoni, Flavio; Bongio, Matilde; Conte, Silvio; Vercesi, Luigi; Cassano, Marco; Tribioli, Carla; Galli, Daniela; Bellazzi, Riccardo; Magenes, Giovanni; Cusella De Angelis, Maria Gabriella; Sampaolesi, Maurilio

    2011-01-01

    We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5 dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5–10.5–11.5 dpc) and cryostat sections for later stages (11.5–13.5–15.5–17.5 dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells. PMID:22187527

  13. Chromosomal rearrangements directly cause underdominant F1 pollen sterility in Mimulus lewisii-Mimulus cardinalis hybrids.

    Science.gov (United States)

    Stathos, Angela; Fishman, Lila

    2014-11-01

    Chromosomal rearrangements can contribute to the evolution of postzygotic reproductive isolation directly, by disrupting meiosis in F1 hybrids, or indirectly, by suppressing recombination among genic incompatibilities. Because direct effects of rearrangements on fertility imply fitness costs during their spread, understanding the mechanism of F1 hybrid sterility is integral to reconstructing the role(s) of rearrangements in speciation. In hybrids between monkeyflowers Mimulus cardinalis and Mimulus lewisii, rearrangements contain all quantitative trait loci (QTLs) for both premating barriers and pollen sterility, suggesting that they may have facilitated speciation in this model system. We used artificial chromosome doubling and comparative mapping to test whether heterozygous rearrangements directly cause underdominant male sterility in M. lewisii-M. cardinalis hybrids. Consistent with a direct chromosomal basis for hybrid sterility, synthetic tetraploid F1 s showed highly restored fertility (83.4% pollen fertility) relative to diploids F1 s (36.0%). Additional mapping with Mimulus parishii-M. cardinalis and M. parishii-M. lewisii hybrids demonstrated that underdominant male sterility is caused by one M. lewisii specific and one M. cardinalis specific reciprocal translocation, but that inversions had no direct effects on fertility. We discuss the importance of translocations as causes of reproductive isolation, and consider models for how underdominant rearrangements spread and fix despite intrinsic fitness costs.

  14. M2-F1 in flight over lakebed on tow line

    Science.gov (United States)

    1963-01-01

    Following the first M2-F1 airtow flight on 16 August 1963, the Flight Research Center used the vehicle for both research flights and to check out new lifting-body pilots. These included Bruce Peterson, Don Mallick, Fred Haise, and Bill Dana from NASA. Air Force pilots who flew the M2-F1 included Chuck Yeager, Jerry Gentry, Joe Engle, Jim Wood, and Don Sorlie, although Wood, Haise, and Engle only flew on car tows. In the three years between the first and last flights of the M2-F1, it made about 400 car tows and 77 air tows. The wingless, lifting body aircraft design was initially concieved as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, Dryden management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and

  15. KERAGAAN PERTUMBUHAN DAN VARIASI GENETIK ABALON Haliotis squamata Reeve (1846 HASIL SELEKSI F-1

    Directory of Open Access Journals (Sweden)

    Gusti Ngurah Permana

    2015-12-01

    Full Text Available Produksi benih abalon Haliotis squamata skala massal di hatcheri telah berhasil dilakukan di Balai Besar Penelitian dan Pengembangan Budidaya Laut Gondol, Bali. Permasalahan utama dalam budidaya abalon adalah pertumbuhan yang lambat. Keadaan tersebut diduga karena pengaruh faktor genetik dan lingkungan. Penelitian ini bertujuan mengetahui keragaan pertumbuhan dan variasi genetik abalon tumbuh cepat hasil seleksi individu. Hasil penelitian ini diketahui bahwa pembentukan populasi F-1 mempunyai pertumbuhan yang lebih baik dengan F-1 kontrol. Peningkatan bobot yang dicapai 22,15 g atau 17,93% lebih baik dibandingkan F-1 kontrol. Keragaman genetik F-1 terseleksi yang ditunjukkan dari nilai heterozigositas adalah (Ho. 0,023 terjadi penurunan 21,7% jika dibandingkan F-0. Hal ini dapat terjadi karena hilangnya beberapa allele dalam proses seleksi. Terdapat hubungan antara jumlah heterozigot pada lokus tertentu dengan pertumbuhan abalon. Hasil ini diharapkan dapat mendukung upaya meningkatkan produksi benih yang mempunyai performa fenotipe dan genotipe unggul sehingga dapat mendukung kegiatan budidaya abalon yang berkelanjutan.

  16. Data of evolutionary structure change: 1BU1F-1OOTA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1BU1F-1OOTA 1BU1 1OOT F A -IIVVALYDYEAIHHEDLSFQKGDQMVVLEES---GEWW...4> 1OOT A 1OOTA ...in> -2.4820001125335693 10.661999702453613 46.814998626708984 ...8 -0.6930000185966492 -0.30300000309944153 tion> 2.081804037094116 A 1OOTA WTGRV--NGREG

  17. E2F1 is crucial for E2F-dependent apoptosis

    DEFF Research Database (Denmark)

    Lazzerini Denchi, Eros; Helin, Kristian

    2005-01-01

    Loss of the retinoblastoma protein, pRB, leads to apoptosis, and several results have suggested that this is dependent on the E2F transcription factors. However, so far, the ability of the different E2F family members to contribute to apoptosis is controversial. Here, we show that ectopic...... expression of E2F3 results in apoptosis in both primary mouse fibroblasts and transgenic mice. Apoptosis induced by E2F3 is associated with the accumulation of E2F1 and, strikingly, we found that E2F3-induced apoptosis is dependent on E2F1. On the basis of these results, we propose that the accumulation...... of crucial levels of E2F1 activity, and not total E2F activity, is essential for the induction of apoptosis in response to a deregulated pRB pathway. These results are consistent with previous findings that E2F1, but not other E2Fs, can have tumour-suppressing activities....

  18. E2F1-mediated transcriptional inhibition of the plasminogen activator inhibitor type 1 gene

    DEFF Research Database (Denmark)

    Koziczak, M; Müller, H; Helin, K;

    2001-01-01

    -sensitive retinoblastoma protein (pRB), a shift to a permissive temperature induced PAI-1 mRNA expression. In U2OS cells stably expressing an E2F1-estrogen receptor chimeric protein that could be activated by tamoxifen, PAI-1 gene transcription was markedly reduced by tamoxifen even in the presence of cycloheximide...

  19. Rooting pattern and nitrogen uptake of three cauliflower (Brassica oleracea var. botrytis) F1-hbrids

    NARCIS (Netherlands)

    Rather, K.; Schenk, M.K.; Everaarts, A.P.; Vethman, S.

    2000-01-01

    In a two-year field trial at the sites Ruthe (Germany, loess soil, Orthic Luvisol) and Schermer (The Netherlands, marine clay soil, Eutric Fluvisol) the cauliflower F1-hybrids Marine, Lindurian and Linford were compared in their efficiency of N use from limiting and optimum supplies of N. Limiting N

  20. A clinical trial to evaluate the safety and immunogenicity of the LEISH-F1+MPL-SE vaccine for use in the prevention of visceral leishmaniasis.

    Science.gov (United States)

    Chakravarty, Jaya; Kumar, Subodh; Trivedi, Sonali; Rai, Vijay K; Singh, Anup; Ashman, Jill A; Laughlin, Elsa M; Coler, Rhea N; Kahn, Stuart J; Beckmann, Anna Marie; Cowgill, Karen D; Reed, Steven G; Sundar, Shyam; Piazza, Franco M

    2011-04-27

    Healthy Indian adult volunteers, with or without a history of leishmaniasis, were evaluated for evidence of previous infection with Leishmania donovani based on the direct agglutination test (DAT). Three cohorts of 6 DAT-negative and 6 DAT-positive subjects were enrolled in an open-label, dose-escalating, uncontrolled clinical trial and received three injections of the LEISH-F1+MPL-SE vaccine (consisting of 5μg, 10μg, or 20μg recombinant Leishmania polyprotein LEISH-F1 antigen+25μg MPL®-SE adjuvant). The study injections were given subcutaneously on days 0, 28, and 56, and the subjects were followed through day 168 for safety and immunological endpoints. The vaccine was safe and well-tolerated in DAT-negative and DAT-positive subjects and induced T-cell production of IFN-γ and other cytokines in response to stimulation with the LEISH-F1 antigen. This clinical trial shows that the LEISH-F1+MPL-SE vaccine is safe and immunogenic in healthy subjects with and without history of previous infection with Leishmania donovani. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. Daphnetin inhibits inflammation in the NZB/W F1 systemic lupus erythematosus murine model via inhibition of NF-κB activity

    Science.gov (United States)

    Li, Min; Shi, Xiaowei; Chen, Fangru; Hao, Fei

    2017-01-01

    Daphnetin is a compound extracted from Chinese medicinal herbs, which exerts analgesic and anti-inflammatory effects. The present study aimed to investigate the potential therapeutic effect of daphnetin on inflammation in the NZB/W F1 systemic lupus erythematosus (SLE) murine model. Female NZB/WF1 mice (age, 16–18 weeks) were intraperitoneally injected with daphnetin once a day for 12 weeks. It was revealed that daphnetin treatment significantly increased animal survival rates, reduced renal damage and blood urea nitrogen levels, and suppressed serum autoantibody production in the SLE-prone NZB/W F1 mice. In addition, daphnetin treatment significantly decreased the serum levels of tumor necrosis factor-α and interleukin-6, inhibited nuclear factor (NF)-κB activity, suppressed the protein expression of nuclear factor of activated T-cells and promoted A20 protein expression in SLE-prone NZB/W F1 mice. In conclusion, daphnetin inhibited inflammation in the NZB/W F1 murine SLE model via inhibition of NF-κB mediated by upregulation of A20.

  2. General combining ability for volume growth of open-pollinated progenies of Korean Pine (Pinus koraiensis

    Directory of Open Access Journals (Sweden)

    S.U. Han

    2013-12-01

    Full Text Available Genetic quality of plus trees selected phenotypically is generally determined by progeny tests in a breeding program. For the progeny test, there are a few limitations; all plus trees are not selected in the same year, seed collection does not occur in the same year due to flowering irregularity, unequal availability of progenies among families, and so on. Such limitations reflect spatial and temporal variation in the establishment of progeny test trials. Thus, one should develop a method of standardization in order to apply genetic information (i.e., general combining ability from the progeny tests into genetic thinning or establishment of improved seed orchard. In this study, we developed a method for standardization to estimate general combining ability (GCA based on the standard-site adjustment system. The method developed took the age of progeny and the number of test sites into account, which was based on the standard deviation of volume index (Height x DBH2 among different ages, sites and replications. Also, we used different weight values depending on the number of test sites and replications, and the age of trials. This method might give a general base on which to generalize the spatial and temporal variation in progeny tests. On the basis of the method, we estimated theGCA of 244 open-pollinated families of Pinus koraiensis that were tested at four sites (Gunpo, Chuncheon, Kangryuong and Chungwonestablished from 1975 to 1994. Among 244 families, KW15 (0115 family showed the highest GCA value, while KW28 (0128 familyrevealed the lowest GCA value. Each family had different GCA values depending on the age of progenies as well as sites. Although there are some needs to consider on gene-ecology for accurate results, the method we reported could be useful to estimate the GCA values of progenies tested under different ages and sites.

  3. General combining ability for volume growth of open-pollinated progenies of Korean Pine (Pinus koraiensis

    Directory of Open Access Journals (Sweden)

    S.U. Han

    2013-12-01

    Full Text Available Genetic quality of plus trees selected phenotypically is generally determined by progeny tests in a breeding program. For the progeny test, there are a few limitations; all plus trees are not selected in the same year, seed collection does not occur in the same year due to flowering irregularity, unequal availability of progenies among families, and so on. Such limitations reflect spatial and temporal variation in the establishment of progeny test trials. Thus, one should develop a method of standardization in order to apply genetic information (i.e., general combining ability from the progeny tests into genetic thinning or establishment of improved seed orchard. In this study, we developed a method for standardization to estimate general combining ability (GCA based on the standard-site adjustment system. The method developed took the age of progeny and the number of test sites into account, which was based on the standard deviation of volume index (Height x DBH2 among different ages, sites and replications. Also, we used different weight values depending on the number of test sites and replications, and the age of trials. This method might give a general base on which to generalize the spatial and temporal variation in progeny tests. On the basis of the method, we estimated theGCA of 244 open-pollinated families of Pinus koraiensis that were tested at four sites (Gunpo, Chuncheon, Kangryuong and Chungwonestablished from 1975 to 1994. Among 244 families, KW15 (0115 family showed the highest GCA value, while KW28 (0128 familyrevealed the lowest GCA value. Each family had different GCA values depending on the age of progenies as well as sites. Although there are some needs to consider on gene-ecology for accurate results, the method we reported could be useful to estimate the GCA values of progenies tested under different ages and sites.

  4. Measurement of the deposited activity of the short-lived radon progeny in the human respiratory tract

    Energy Technology Data Exchange (ETDEWEB)

    Vezzu, G.; Butterweck-Dempewolf, G.; Schuler, C. [Paul Scherrer Inst., Villigen (Switzerland). Div. for Radiation Protection and Waste Management

    1998-12-31

    Volunteers were exposed in the radon chamber at Paul Scherrer Institut to an atmosphere enriched with highly unattached radon progeny. The deposited radon progeny activity in the respiratory tract of the volunteers was determined using a low level in-vivo counter. The detector arrangement and its calibration for the measurement of deposited radon progeny activity is described and the results for a mouth and a nose breathing volunteer are presented. For the nose breathing volunteer 55% of the deposited radon progeny activity was located in the head and the remaining 45% in the chest whereas for the mouth breathing volunteer 25% was located in the head and the remaining 75% in the chest. A mean clearance half-life for the deposited radon progeny from the respiratory tract of (2{+-}1) h was obtained from the analyses of the temporal behaviour of the deposited radon progeny activity in the head. (orig.)

  5. Assessment of the unattached fraction of indoor radon progeny and its contribution to dose: a pilot study in China.

    Science.gov (United States)

    Guo, Qiuju; Zhang, Lei; Guo, Lu

    2012-12-01

    The unattached fraction of radon progeny (f(p)) is one of the most important factors for accurate evaluation of the effective dose from a unit of radon exposure, and it may vary greatly in different environments. For precise evaluation of the indoor radon exposure dose and the influence of unattached radon progeny, a pilot survey of f(p) in different environments was carried out in China with a portable and integrating monitor. The dose conversion factors for radon progeny are calculated with LUDEP(®) code, and the dose contributions from the unattached and the attached radon progenies were simultaneously evaluated based on the results of field measurements. The results show that even though the concentrations of radon progeny vary significantly among different indoor environments, the variations of f(p) seem relatively small (9.3-16.9%). The dose contribution from unattached radon progeny is generally larger (30.2-46.2%) in an indoor environment.

  6. Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus).

    Science.gov (United States)

    Kwon, Hyuk Woo; Choi, Min Ah; Yun, Yeo Hong; Oh, Youn-Lee; Kong, Won-Sik; Kim, Seong Hwan

    2015-03-01

    To promote the selection of promising monokaryotic strains of button mushroom (Agaricus bisporus) during breeding, 61 progeny strains derived from basidiospores of two different lines of dikaryotic parental strains, ASI1038 and ASI1346, were analyzed by nucleotide sequencing of the intergenic spacer I (IGS I) region in their rDNA and by extracellular enzyme assays. Nineteen different sizes of IGS I, which ranged from 1,301 to 1,348 bp, were present among twenty ASI1346-derived progeny strains, while 15 different sizes of IGS I, which ranged from 700 to 1,347 bp, were present among twenty ASI1038-derived progeny strains. Phylogenetic analysis of the IGS sequences revealed that different clades were present in both the ASI10388- and ASI1346-derived progeny strains. Plating assays of seven kinds of extracellular enzymes (β-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease) also revealed apparent variation in the ability to produce extracellular enzymes among the 40 tested progeny strains from both parental A. bisporus strains. Overall, this study demonstrates that characterization of IGS I regions and extracellular enzymes is useful for the assessment of the substrate-degrading ability and heterogenicity of A. bisporus monokaryotic strains.

  7. Variability of Norway spruce morphometric characteristics in progeny tests in Bosnia and Herzegovina

    Directory of Open Access Journals (Sweden)

    Cvjetković Branislav

    2016-01-01

    Full Text Available The paper presents the results of an analysis of morphometric characteristics of Norway spruce seedlings in the progeny tests in Bosnia and Herzegovina, localities: Drinić and Srebrenica. Progeny tests were established by using seedlings originating from six natural populations from Bosnia and Herzegovina. Seedling height, root collar and whorl distance from the ground, which represents seedling heights in 2011, 2012 and 2013 were measured. The results indicate statistically significant differences between the analyzed characteristics of the observed population. Especially interesting are the populations Potoci and Olovo, which showed the highest growth during the observed period in both progeny tests. The impact of the drought in 2013 can be seen in the progeny test in Srebrenica where height growth in 2013 was lower by more than 50% in comparison to 2012. The impact of drought in the progeny test in Drinić was not observed. The research provides the baseline when selecting the starting population, where the rapid growth of seedlings is set as the main goal.

  8. Dose estimation derived from the exposure to radon, thoron and their progeny in the indoor environment

    Science.gov (United States)

    Ramola, R. C.; Prasad, Mukesh; Kandari, Tushar; Pant, Preeti; Bossew, Peter; Mishra, Rosaline; Tokonami, S.

    2016-08-01

    The annual exposure to indoor radon, thoron and their progeny imparts a major contribution to inhalation doses received by the public. In this study, we report results of time integrated passive measurements of indoor radon, thoron and their progeny concentrations that were carried out in Garhwal Himalaya with the aim of investigating significant health risk to the dwellers in the region. The measurements were performed using recently developed LR-115 detector based techniques. The experimentally determined values of radon, thoron and their progeny concentrations were used to estimate total annual inhalation dose and annual effective doses. The equilibrium factors for radon and thoron were also determined from the observed data. The estimated value of total annual inhalation dose was found to be 1.8 ± 0.7 mSv/y. The estimated values of the annual effective dose were found to be 1.2 ± 0.5 mSv/y and 0.5 ± 0.3 mSv/y, respectively. The estimated values of radiation doses suggest no important health risk due to exposure of radon, thoron and progeny in the study area. The contribution of indoor thoron and its progeny to total inhalation dose ranges between 13–52% with mean value of 30%. Thus thoron cannot be neglected when assessing radiation doses.

  9. Phenotypic and Behavioral Defects Induced by Iron Exposure Can Be Transferred to Progeny in Caenorhabditis elegans

    Institute of Scientific and Technical Information of China (English)

    YA-OU HU; YANG WANG; BO-PING YE; DA-YONG WANG

    2008-01-01

    Previous work has showed that excess iron accumulation is harmful to reproduction and even promotes death;however,whether the multiple biological toxicity of iron (Fe) exposure could be transferred to progeny remains unknown.The present study used Caenorhabditis elegans to analyze the multiple toxicities of iron exposure and their possible transferable properties.Methods Three concentrations of iron sulfate solution (2.5μmol/L,75μmol/L,and 200 lamol/L) were used.The endpoints of lifespan,body size,generation time,brood size,head thrash and body bend frequencies,and chemotaxis plasticity were selected to investigate Fe toxicity and its effect on progeny in Caenorhabditis elegans.Results The Fe toxicity could cause multiple biological defects in a dose-dependent manner by affecting different endpoints in nematodes.Most of the multiple biological defects and behavior toxicities could be transferred from Fe-exposed Caenorhabditis elegans to their progeny.Compared to the parents,no recovery phenotypes were observed for some of the defects in the progeny,such as body bend frequency and life span.We further summarized the defects caused by Fe exposure into 2 groups according to their transferable properties.Conclusion Our results suggest that Fe exposure could cause multiple biological defects,and most of these severe defects could be transferred from Fe exposed nematodes to their progeny.

  10. Dose estimation derived from the exposure to radon, thoron and their progeny in the indoor environment

    Science.gov (United States)

    Ramola, R. C.; Prasad, Mukesh; Kandari, Tushar; Pant, Preeti; Bossew, Peter; Mishra, Rosaline; Tokonami, S.

    2016-01-01

    The annual exposure to indoor radon, thoron and their progeny imparts a major contribution to inhalation doses received by the public. In this study, we report results of time integrated passive measurements of indoor radon, thoron and their progeny concentrations that were carried out in Garhwal Himalaya with the aim of investigating significant health risk to the dwellers in the region. The measurements were performed using recently developed LR-115 detector based techniques. The experimentally determined values of radon, thoron and their progeny concentrations were used to estimate total annual inhalation dose and annual effective doses. The equilibrium factors for radon and thoron were also determined from the observed data. The estimated value of total annual inhalation dose was found to be 1.8 ± 0.7 mSv/y. The estimated values of the annual effective dose were found to be 1.2 ± 0.5 mSv/y and 0.5 ± 0.3 mSv/y, respectively. The estimated values of radiation doses suggest no important health risk due to exposure of radon, thoron and progeny in the study area. The contribution of indoor thoron and its progeny to total inhalation dose ranges between 13–52% with mean value of 30%. Thus thoron cannot be neglected when assessing radiation doses. PMID:27499492

  11. Direct measurement of attachment of {sup 220}Rn progeny on aerosols by atomic force microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Leung, J.K.C. E-mail: jkcleung@hku.hk; Tso, M.Y.W.; Lam, J.H.C.; Zhau, Q.F

    2003-08-11

    Atomic force microscopy (AFM) is becoming a powerful tool for the study of nuclear tracks in materials such as CR-39. Coupled with its capability of observing near nm aerosol particles, we have utilized the AFM to observe the radon progeny-loaded aerosol particles deposited on surfaces of CR-39 and to observe the corresponding etch pits produced by the {alpha}-particles emitted from the radon progenies. A special platform was built so that after the aerosol particles on the CR-39 have been scanned and recorded, the CR-39 can be etched and then scanned for the etch pits at the same location. Both {sup 222}Rn and {sup 220}Rn progenies were used in the study. The progenies were generated by the appropriate radon sources and mixed with aerosol particles generated by aerosol generators. The aerosol size distributions were analyzed by a scanning mobility particle sizer. Some of the limitations and difficulties of the technique will be described. The results enable us to examine the attachment process including multiple attachments of radon progenies on aerosols.

  12. Properties of F1-ATPase from the uncD412 mutant of Escherichia coli.

    Science.gov (United States)

    Wise, J G; Duncan, T M; Latchney, L R; Cox, D N; Senior, A E

    1983-11-01

    Properties of purified F1-ATPase from Escherichia coli mutant strain AN484 (uncD412) have been studied in an attempt to understand why the amino acid substitution in the beta-subunit of this enzyme causes a tenfold reduction from normal MgATP hydrolysis rate. In most properties that were studied, uncD412 F1-ATPase resembled normal E. coli F1-ATPase. Both enzymes were found to contain a total of six adenine-nucleotide-binding sites, of which three were found to be non-exchangeable and three were exchangeable (catalytic) sites. Binding of the non-hydrolysable substrate analogue adenosine 5'-[beta gamma-imido]triphosphate (p[NH]ppA) to the three exchangeable sites showed apparent negative co-operativity. The binding affinities for p[NH]ppA, and also ADP, at the exchangeable sites were similar in the two enzymes. Both enzymes were inhibited by efrapeptin, aurovertin and p[NH]ppA, and were inactivated by dicyclohexylcarbodi-imide, 4-chloro-7-nitrobenzofurazan and p-fluorosulphonyl-benzoyl-5'-adenosine. Km values for CaATP and MgATP were similar in the two enzymes. uncD412 F1-ATPase was abnormally unstable at high pH, and dissociated into subunits readily with consequent loss of activity. The reason for the impairment of catalysis in uncD412 F1-ATPase cannot be stated with certainty from these studies. However we discuss the possibility that the mutation interrupts subunit interaction, thereby causing a partial impairment in the site-site co-operativity which is required for 'promotion' of catalysis in this enzyme.

  13. Study on the polymorphism of POU1F1 gene in sheep

    Directory of Open Access Journals (Sweden)

    Jun Yan Bai

    Full Text Available ABSTRACT In this study, POU1F1 gene polymorphism was detected in five sheep populations (large-tailed Han, small-tailed Han, Yuxi fat-tailed, Lanzhou large-tailed, and Mongolian sheep, using DNA pooling and sequencing, to provide theoretical basis for the breeding of excellent sheep varieties. Three single-nucleotide polymorphism (SNP loci of POU1F1 gene were detected in five sheep populations, namely C355T (C/T, C71G (C/G, and C330G (C/G. C and T frequencies of C355T were 0.67/0.33, 0.81/0.19, 0.67/0.33, 1.00/0.00, and 0.93/0.07, respectively, in large-tailed Han, small-tailed Han, Yuxi fat-tailed, Mongolian, and Lanzhou large-tailed sheep. C of C355T locus was the dominant allele in five sheep populations. C and G allele frequencies of C330G locus were detected in Yuxi fat-tailed sheep; their frequencies were 0.75 and 0.25, respectively. C and G allele of C71G locus were only detected in Yuxi fat-tailed and large-tailed Han sheep; their frequencies were 0.87/0.13 and 0.87/0.13, respectively. The cluster analysis based on POU1F1 gene sequence showed that bactrian camel, dromedary, and wild camel clustered first, and dolphin and killer whales clustered according to taxonomy. Although the four species Tibetan antelope, buffalo, goat, and sheep were alone, they got close and the relative genetic relationship was intimate according to the dendrogram. The mutation site analysis of the POU1F1 gene in five sheep populations in this study would be favorable for uncovering the function of POU1F1 gene deeply.

  14. Resveratrol enhances the radiosensitivity of nasopharyngeal carcinoma cells by downregulating E2F1.

    Science.gov (United States)

    Tan, Yuhui; Wei, Xianli; Zhang, Wenyin; Wang, Xiaolan; Wang, Kun; Du, Biaoyan; Xiao, Jianyong

    2017-03-01

    Identification of safe, effective radiosensitizing agents is urgently needed to improve the outcome of radiotherapy in nasopharyngeal cancer (NPC). In this study, we assessed the ability of the polyphenol resveratrol to act as a radiosensitizer in vitro and in vivo. CNE-1 cells were treated with 50 µM resveratrol for 24 h, then irradiated. E2F transcription factor 1 (E2F1) was stably knocked down and overexpressed using lentiviruses. A xenograft model of NPC was established in nude mice using CNE-1 cells. Compared to control DMSO‑treated CNE-1 cells, resveratrol inhibited colony-forming ability and induced G1 phase cell cycle arrest. Radiation survival curves confirmed resveratrol significantly sensitized CNE-1 cells, and resveratrol in combination with 2 Gy irradiation synergistically increased apoptosis. Immunoblotting showed resveratrol dose- and time-dependently downregulated E2F1 and phospho-AKT (p-AKT). Knockdown of E2F1 significantly increased radiosensitivity and downregulated p-AKT; overexpression of E2F1 reversed resveratrol-induced radiosensitivity and upregulated p-AKT. In vivo, 50 mg/kg/day resveratrol and 4 Gy irradiation led to significantly lower tumor volume and tumor weight compared to resveratrol or irradiation alone. Our findings show that resveratrol increases the radiosensitivity of NPC cells by downregulating E2F1 and inhibiting p-AKT, and therefore has potential as a radiosensitizer for NPC.

  15. Varietal differences in photosynthetic characters and chlorophyll fluorescence induction kinetics parameters among intergeneric progeny derived from Oryza×Sorghum, its parents, and hybrid rice

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A comparative study on the photosynthetic parameters among intergeneric progenies derived from Oryza sativa L.×Sorghum vulgare L., its maternal parent Gui 630 and commercial 3-line hybrid rice Shanyou 63 in pot experiment in greenhouse was conducted. The morphological and photosynthetic characters of canopy leaves and chlorophyll fluorescence kinetic parameters including Fv/Fm, Fv/F0, photochemical quenching coefficient and non-photochemical coefficient of canopy leaves of 3 varieties were measured. The results showed the progeny, Yuanyou 1, derived from an intergeneric cross of rice and sorghum possesses better canopy spatial architecture with thicker, heavier and bigger canopy leaf than its maternal parent Gui 630. Higher photosynthetic rate due to higher chlorophyll content, higher primary energy transformation efficiency, potential of PSII and non-photochemical quenching coefficient (qE) were also measured in Yuanyou 1. These explain partly why the intergeneric progeny has higher biomass production, and better tolerance to adverse conditions and higher field yields even under stress conditions.

  16. Varietal differences in photosynthetic characters and chlorophyll fluorescence induction kinetics parameters among intergeneric progeny derived from Oryza×Sorghum, its parents, and hybrid rice

    Institute of Scientific and Technical Information of China (English)

    KatsuyoshiShimizu; 唐建军; 陈欣

    2002-01-01

    A comparative study on the photosynthetic parameters among intergeneric progenies derived from Oryza sativa L.× Sorghum vulgare L. , its maternal parent Gui 630 and commercial 3-line hybrid rice Shanyou 63 in pot experiment in greenhouse was conducted. The morphological and photosynthetic characters of canopy leaves and chlorophyll fluorescence kinetic pm'mneters including Fv/Fm, Fv/F0, photochemical quenching coefficient and non-photochemical coefficient of canopy leaves of 3 varieties were measured. The results showed the progeny, Yuanyou 1, derived from an intergeneric cross of rice and sorghum possesses better canopy spatial architecture with thicker, heavier and bigger canopy leaf than its maternal parent Gui 630.Higher photosynthetic rate due to higher chlorophyll content, higher primary energy transformation efficiency,potential of PSII and non-photochemieal quenching coefficient (qE) were also measured in Yuanyou 1. These explain partly why the intergeneric progeny has higher biomass production, and better tolerance to adverse conditions and higher field yields even under stress conditions.

  17. Development of a genome-wide multiple duplex-SSR protocol and its applications for the identification of selfed progeny in switchgrass

    Directory of Open Access Journals (Sweden)

    Liu Linglong

    2012-10-01

    Full Text Available Abstract Background Switchgrass (Panicum virgatum is a herbaceous crop for the cellulosic biofuel feedstock development in the USA and Europe. As switchgrass is a naturally outcrossing species, accurate identification of selfed progeny is important to producing inbreds, which can be used in the production of heterotic hybrids. Development of a technically reliable, time-saving and easily used marker system is needed to quantify and characterize breeding origin of progeny plants of targeted parents. Results Genome-wide screening of 915 mapped microsatellite (simple sequence repeat, SSR markers was conducted, and 842 (92.0% produced clear and scorable bands on a pooled DNA sample of eight switchgrass varieties. A total of 166 primer pairs were selected on the basis of their relatively even distribution in switchgrass genome and PCR amplification quality on 16 tetraploid genotypes. Mean polymorphic information content value for the 166 markers was 0.810 ranging from 0.116 to 0.959. From them, a core set of 48 loci, which had been mapped on 17 linkage groups, was further tested and optimized to develop 24 sets of duplex markers. Most of (up to 87.5% targeted, but non-allelic amplicons within each duplex were separated by more than 10-bp. Using the established duplex PCR protocol, selfing ratio (i.e., selfed/all progeny x100% was identified as 0% for a randomly selected open-pollinated ‘Kanlow’ genotype grown in the field, 15.4% for 22 field-grown plants of bagged inflorescences, and 77.3% for a selected plant grown in a growth chamber. Conclusions The study developed a duplex SSR-based PCR protocol consisting of 48 markers, providing ample choices of non-tightly-linked loci in switchgrass whole genome, and representing a powerful, time-saving and easily used method for the identification of selfed progeny in switchgrass. The protocol should be a valuable tool in switchgrass breeding efforts.

  18. E2F1 promote the aggressiveness of human colorectal cancer by activating the ribonucleotide reductase small subunit M2

    Energy Technology Data Exchange (ETDEWEB)

    Fang, Zejun [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China); Gong, Chaoju [Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058 (China); Liu, Hong [Zhejiang Normal University – Jinhua People' s Hospital Joint Center for Biomedical Research, Jinhua, Zhejiang, 321004 (China); Zhang, Xiaomin; Mei, Lingming [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China); Song, Mintao [Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS), School of Basic Medicine, Peking Union Medical College (PUMC), Beijing, 100005 (China); Qiu, Lanlan; Luo, Shuchai; Zhu, Zhihua; Zhang, Ronghui; Gu, Hongqian [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China); Chen, Xiang, E-mail: sychenxiang@126.com [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China)

    2015-08-21

    As the ribonucleotide reductase small subunit, the high expression of ribonucleotide reductase small subunit M2 (RRM2) induces cancer and contributes to tumor growth and invasion. In several colorectal cancer (CRC) cell lines, we found that the expression levels of RRM2 were closely related to the transcription factor E2F1. Mechanistic studies were conducted to determine the molecular basis. Ectopic overexpression of E2F1 promoted RRM2 transactivation while knockdown of E2F1 reduced the levels of RRM2 mRNA and protein. To further investigate the roles of RRM2 which was activated by E2F1 in CRC, CCK-8 assay and EdU incorporation assay were performed. Overexpression of E2F1 promoted cell proliferation in CRC cells, which was blocked by RRM2 knockdown attenuation. In the migration and invasion tests, overexpression of E2F1 enhanced the migration and invasion of CRC cells which was abrogated by silencing RRM2. Besides, overexpression of RRM2 reversed the effects of E2F1 knockdown partially in CRC cells. Examination of clinical CRC specimens demonstrated that both RRM2 and E2F1 were elevated in most cancer tissues compared to the paired normal tissues. Further analysis showed that the protein expression levels of E2F1 and RRM2 were parallel with each other and positively correlated with lymph node metastasis (LNM), TNM stage and distant metastasis. Consistently, the patients with low E2F1 and RRM2 levels have a better prognosis than those with high levels. Therefore, we suggest that E2F1 can promote CRC proliferation, migration, invasion and metastasis by regulating RRM2 transactivation. Understanding the role of E2F1 in activating RRM2 transcription will help to explain the relationship between E2F1 and RRM2 in CRC and provide a novel predictive marker for diagnosis and prognosis of the disease. - Highlights: • E2F1 promotes RRM2 transactivation in CRC cells. • E2F1 promotes the proliferation of CRC cells by activating RRM2. • E2F1 promotes the migration and

  19. Hybrid Weakness Found in the Backcross Progeny of an Inter-Specific Cross of Oryza Sativa / O. Glumaepatula

    Directory of Open Access Journals (Sweden)

    Sobrizal

    2009-01-01

    Full Text Available Hybrid breakdown expressed as sterility or weakness or both in F2 or later generations is considered to be a reproductive barrier between two distantly related taxa. Hybrid breakdown that showed poor growth habit with fertile seeds was found in the backcross progenies (BC4F2 which were derived from a cross between a cultivated rice Oryza sativa L. cv. Taichung 65 and a wild rice Oryza glumaepatula Steud (Acc. IRGC 105668 where Taichung 65 was used as a recurrent parent. Through segregation analysis of BC4F2 population, a novel gene for weakness was identified, and this gene was designated as hwf1 (hybrid weakness f-1. RFLP (Restriction Fragment Length Polymorphism analysis was employed to determine the chromosomal location of hwf1 gene. The result revealed that hwf1 was located between G3006 and C933 RFLP markers, with map distances of 0.5 cM and 1.9 cM, respectively, and co-segregated with C708, C820, and R288 on the short arm of chromosome 4. Since both O. glumaepatula (Acc. IRGC 105668 and Taichung 65 that was used as parents showed normal growth habit, the weakness in this study was assumed to be controlled by a set of complementary recessive genes. One of the complementary genes is hwf1 from O. glumaepatula, and another gene should be from Taichung 65, because the genetic background of the BC4F2 populations that were used for analysis is Taichung 65. The gene from Taichung 65 has not been identified yet, therefore, it is necessary to conduct the experiment for finding out the complement of hwf1 gene in near future.

  20. Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna.

    Directory of Open Access Journals (Sweden)

    Nur Syafiqah Mohamad Ishak

    Full Text Available Identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. Orthologs of Drosophila orphan receptor Fushi tarazu factor-1 (Ftz-F1 are known to function in genetic sex determination. In contrast, their roles in environmental sex determination remain unknown. In this study, we have cloned and characterized the Ftz-F1 ortholog in the branchiopod crustacean Daphnia magna, which produces males in response to environmental stimuli. Similar to that observed in Drosophila, D. magna Ftz-F1 (DapmaFtz-F1 produces two splicing variants, αFtz-F1 and βFtz-F1, which encode 699 and 777 amino acids, respectively. Both isoforms share a DNA-binding domain, a ligand-binding domain, and an AF-2 activation domain and differ only at the A/B domain. The phylogenetic position and genomic structure of DapmaFtz-F1 suggested that this gene has diverged from an ancestral gene common to branchiopod crustacean and insect Ftz-F1 genes. qRT-PCR showed that at the one cell and gastrulation stages, both DapmaFtz-F1 isoforms are two-fold more abundant in males than in females. In addition, in later stages, their sexual dimorphic expressions were maintained in spite of reduced expression. Time-lapse imaging of DapmaFtz-F1 RNAi embryos was performed in H2B-GFP expressing transgenic Daphnia, demonstrating that development of the RNAi embryos slowed down after the gastrulation stage and stopped at 30-48 h after ovulation. DapmaFtz-F1 shows high homology to insect Ftz-F1 orthologs based on its amino acid sequence and exon-intron organization. The sexually dimorphic expression of DapmaFtz-F1 suggests that it plays a role in environmental sex determination of D. magna.

  1. Purification, crystallization, and properties of F1-ATPase complexes from the thermoalkaliphilic Bacillus sp. strain TA2.A1.

    Science.gov (United States)

    Stocker, Achim; Keis, Stefanie; Cook, Gregory M; Dimroth, Peter

    2005-11-01

    Recently, we reported the cloning of the atp operon encoding for the F(1)F(0)-ATP synthase from the extremely thermoalkaliphilic bacterium Bacillus sp. strain TA2.A1. In this study, the genes encoding the F(1) moiety of the enzyme complex were cloned from the atp operon into the vector pTrc99A and expressed in Escherichia coli in two variant complexes, F(1)-wt consisting of subunits alpha(3)beta(3)gammadeltaepsilon and F(1)Deltadelta lacking the entire delta-subunit as a prerequisite for overproduction and crystallization trials. Both F(1)-wt and F(1)Deltadelta were successfully overproduced in E. coli and purified in high yield and purity. F(1)Deltadelta was crystallized by micro-batch screening yielding three-dimensional crystals that diffracted to a resolution of 3.1A using a synchrotron radiation source. After establishing cryo and dehydrating conditions, a complete set of diffraction data was collected from a single crystal. No crystals were obtained with F(1)-wt. Data processing of diffraction patterns showed that F(1)Deltadelta crystals belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell parameters of a=121.70, b=174.80, and c=223.50A, alpha, beta, gamma=90.000. The asymmetric unit contained one molecule of bacterial F(1)Deltadelta with a corresponding volume per protein weight (V(M)) of 3.25A(3) Da(-1) and a solvent content of 62.1%. Silver staining of single crystals of F(1)Deltadelta analyzed by SDS-PAGE revealed four bands alpha, beta, gamma, and epsilon with identical M(r)-values as those found in the native F(1)F(0)-ATP synthase isolated from strain TA2.A1 membranes. ATPase assays of F(1)Deltadelta crystals exhibited latent ATP hydrolytic activity that was highly stimulated by lauryldimethylamine oxide, a hallmark of the native enzyme.

  2. Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna.

    Science.gov (United States)

    Mohamad Ishak, Nur Syafiqah; Kato, Yasuhiko; Matsuura, Tomoaki; Watanabe, Hajime

    2016-01-01

    Identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. Orthologs of Drosophila orphan receptor Fushi tarazu factor-1 (Ftz-F1) are known to function in genetic sex determination. In contrast, their roles in environmental sex determination remain unknown. In this study, we have cloned and characterized the Ftz-F1 ortholog in the branchiopod crustacean Daphnia magna, which produces males in response to environmental stimuli. Similar to that observed in Drosophila, D. magna Ftz-F1 (DapmaFtz-F1) produces two splicing variants, αFtz-F1 and βFtz-F1, which encode 699 and 777 amino acids, respectively. Both isoforms share a DNA-binding domain, a ligand-binding domain, and an AF-2 activation domain and differ only at the A/B domain. The phylogenetic position and genomic structure of DapmaFtz-F1 suggested that this gene has diverged from an ancestral gene common to branchiopod crustacean and insect Ftz-F1 genes. qRT-PCR showed that at the one cell and gastrulation stages, both DapmaFtz-F1 isoforms are two-fold more abundant in males than in females. In addition, in later stages, their sexual dimorphic expressions were maintained in spite of reduced expression. Time-lapse imaging of DapmaFtz-F1 RNAi embryos was performed in H2B-GFP expressing transgenic Daphnia, demonstrating that development of the RNAi embryos slowed down after the gastrulation stage and stopped at 30-48 h after ovulation. DapmaFtz-F1 shows high homology to insect Ftz-F1 orthologs based on its amino acid sequence and exon-intron organization. The sexually dimorphic expression of DapmaFtz-F1 suggests that it plays a role in environmental sex determination of D. magna.

  3. M2-F1 in flight during low-speed car tow

    Science.gov (United States)

    1963-01-01

    The M2-F1 shown in flight during a low-speed car tow runs across the lakebed. Such tests allowed about two minutes to test the vehicle's handling in flight. NASA Flight Research Center (later redesignated the Dryden Flight Research Center) personnel conducted as many as 8 to 14 ground-tow flights in a single day either to test the vehicle in preparation for air tows or to train pilots to fly the vehicle before they undertook air tows. The wingless, lifting body aircraft design was initially concieved as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, Dryden management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and racing slicks, the Pontiac could tow the 1,000-pound M2-F1 110 miles per hour in 30

  4. A New Approach to Modify Plant Microbiomes and Traits by Introducing Beneficial Bacteria at Flowering into Progeny Seeds.

    Science.gov (United States)

    Mitter, Birgit; Pfaffenbichler, Nikolaus; Flavell, Richard; Compant, Stéphane; Antonielli, Livio; Petric, Alexandra; Berninger, Teresa; Naveed, Muhammad; Sheibani-Tezerji, Raheleh; von Maltzahn, Geoffrey; Sessitsch, Angela

    2017-01-01

    The microbial component of healthy seeds - the seed microbiome - appears to be inherited between plant generations and can dynamically influence germination, plant performance, and survival. As such, methods to optimize the seed microbiomes of major crops could have far-reaching implications for plant breeding and crop improvement to enhance agricultural food, feed, and fiber production. Here, we describe a new approach to modulate seed microbiomes of elite crop seed embryos and concomitantly design the traits to be mediated by seed microbiomes. Specifically, we discovered that by introducing the endophyte Paraburkholderia phytofirmans PsJN to the flowers of parent plants we could drive its inclusion in progeny seed microbiomes, thereby inducing vertical inheritance to the offspring generation. We demonstrated the introduction of PsJN to seeds of monocot and dicot plant species and the consequential modifications to seed microbiome composition and growth traits in wheat, illustrating the potential role of novel seed-based microbiomes in determining plant traits.

  5. Comparison of noble fir progeny from US Pacific Northwest collection regions and Denmark for Christmas tree traits

    DEFF Research Database (Denmark)

    Landgren, Chal; Nielsen, Ulrik Braüner; Chastagner, Gary

    2017-01-01

    positively correlated while colour showed little correlation with either trait. Provenances from the Oregon Coast and Willapa Hills, Washington provided the highest potential gain for Christmas tree market value. Provenances from Denmark exhibited superior colour and slow growth. For breeding purposes......Noble fir is the leading Christmas tree produced in the US Pacific Northwest (PNW). This study is based on evaluations of 24,721 trees collected from five native collection regions in Oregon and Washington plus imported seed from Denmark. The major collection areas were further subdivided...... into provenances utilizing 16 in the PNW and 6 from Denmark. A total of 22 test sites were evaluated from progeny trials planted between 1996 and 2007. Traits important for commercial Christmas tree production were evaluated and include commercial height, tree grade, value and colour. Tree height and quality were...

  6. Study of the atmospheric chemistry of radon progeny in laboratory and real indoor atmospheres

    Energy Technology Data Exchange (ETDEWEB)

    Hopke, P.K.

    1992-07-01

    This report covers the second year of the 28 month grant current grant to Clarkson University to study the chemical and physical behavior of the polonium 218 atom immediately following its formation by the alpha decay of radon. Because small changes in size for activity result in large changes in the delivered dose per unit exposure, this behavior must be understood if the exposure to radon progeny and it dose to the cells in the respiratory tract are to be fully assessed. Two areas of radon progeny behavior are being pursued; laboratory studies under controlled conditions to better understand the fundamental physical and chemical process that affect the progeny's atmospheric behavior and studies in actual indoor environments to develop a better assessment of the exposure of the occupants of that space to the size and concentration of the indoor radioactive aerosol. This report describes the progress toward achieving these objectives.

  7. Comparative study of various techniques for environmental radon, thoron and progeny measurements.

    Science.gov (United States)

    Ramola, R C; Prasad, Mukesh; Rawat, Mukesh; Dangwal, Anoop; Gusain, G S; Mishra, Rosaline; Sahoo, S K; Tokonami, S

    2015-11-01

    Long-term average concentrations of radon, thoron and progeny were measured in normal and high background radiation areas in India using different techniques. Radon, thoron and progeny concentrations were measured using Raduet, Pin-Hole dosimeter, deposition-based CR-39 and deposition-based direct radon/thoron progeny sensor (DRPS/DTPS) detector system. All these techniques were used at a same time inside an individual dwelling. Radon concentration was recorded higher than thoron concentration in Garhwal Homes (NBRA) while thoron concentration was found relatively higher in the houses of Chhatarpur area (HBRA) in Odisha, India. The values measured with the CR-39 detector-based technique were found comparable with the values measured with the LR-115 detector-based technique. The comparisons of results using various techniques and their usefulness in radiation measurements are discussed in detail.

  8. Further examination of seventeen mutations in Escherichia coli F1-ATPase beta-subunit.

    Science.gov (United States)

    Senior, A E; al-Shawi, M K

    1992-10-25

    Seventeen mutations in beta-subunit of Escherichia coli F1-ATPase which had previously been characterized in strain AN1272 (Mu-induced mutant) were expressed in strain JP17 (beta-subunit gene deletion). Six showed unchanged behavior, namely: C137Y; G142D; G146S; G207D; Y297F; and Y354F. Five failed to assemble F1F0 correctly, namely: G149I; G154I; G149I,G154I; G223D; and P403S,G415D. Six assembled F1F0 correctly, but with membrane ATPase lower than in AN1272, namely: K155Q; K155E; E181Q; E192Q; D242N; and D242V. AN1272 was shown to unexpectedly produce a small amount of wild-type beta-subunit; F1-ATPase activities reported previously in AN1272 were referable to hybrid enzymes containing both mutant and wild-type beta-subunits. Purified F1 was obtained from K155Q; K155E; E181Q; E192Q; and D242N mutants in JP17. Vmax ATPase values were lower, and unisite catalysis rate and equilibrium constants were perturbed to greater extent, than in AN1272. However, general patterns of perturbation revealed by difference energy diagrams were similar to those seen previously, and the new data correlated well in linear free energy relationships for reaction steps of unisite catalysis. Correlation between multisite and unisite ATPase activity was seen in the new enzymes. Overall, the data give strong support to previously proposed mechanisms of unisite catalysis, steady-state catalysis, and energy coupling in F1-ATPases (Al-Shawi, M. K., Parsonage, D. and Senior, A. E. (1990) J. Biol. Chem. 265, 4402-4410). The K155Q, K155E, D242N, and E181Q mutations caused 5000-fold, 4000-fold, 1800-fold, and 700-fold decrease, respectively, in Vmax ATPase, implying possibly direct roles for these residues in catalysis. Experiments with the D242N mutant suggested a role for residue beta D242 in catalytic site Mg2+ binding.

  9. AFLP marking and polymorphism among progenies of Gymnema sylvestre: an important medicinal plant of India.

    Science.gov (United States)

    Osman, Magda Abbaker; Dhawan, Sunita Singh; Bahl, Janak Raj; Darokar, Mahendra P; Khanuja, Suman P S

    2011-11-01

    The level of polymorphism among twelve selected progenies of Gymnema sylvestre was investigated through AFLP markers by multiplexing PCR reactions using 64 (8x8) primer combinations. Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 progenies with these 14 primer pairs produced 1689 fragments of which 972 (57.5%) were polymorphic and 485 (28.7%) were unique to a particular genotype. The number of fragments produced by individual primer pairs was in the range of 55 to 225. Out of these, polymorphic fragments were in the range of 34 (E-ACC/M-CAC) to 157 (E-AGG/M-CAG) and unique bands observed were 8 (E-ACC / M-CAC) to 69 (E-AGG/M-CAC). Different primer combinations detected different levels of polymorphism, ranging from 33% (E-AGG/ M-CAC) to 69.8% (E-AGG/ M-CAC). From the observations, it appears that the primer combinations E-AGG/M-CAC, E-AGG/CTG, E-AGG/CAG and E-ACA/CAT were the most informative for the detection of polymorphism among the progenies compared with others, since they produced a high number of unique fragments. The similarity coefficient ranged from 0.212 to 0.731. High similarity was observed between progeny S8 and S9 (73%) and high divergence between progenies S3 and S11. Among the selected progeny, S9 was found to be the most similar to the parent (63%), while genotype S11 was the most distant (36.9%).

  10. Male reproductive toxicity of CrVI: In-utero exposure to CrVI at the critical window of testis differentiation represses the expression of Sertoli cell tight junction proteins and hormone receptors in adult F1 progeny rats.

    Science.gov (United States)

    Kumar, Kathiresh M; Aruldhas, Mariajoseph Michael; Banu, Sheerin L; Sadasivam, Balaji; Vengatesh, Ganapathy; Ganesh, Karthik M; Navaneethabalakrishnan, Shobana; Navin, Ajith Kumar; Michael, Felicia Mary; Venkatachalam, Sankar; Stanley, Jone A; Ramachandran, Ilangovan; Banu, Sakhila K; Akbarsha, Mohammad Abdulkader

    2017-02-10

    The effect of gestational exposure to CrVI (occupational/environmental pollutant and target to Sertoli cells(SC)) was tested in a rat model during the testicular differentiation from the bipotential gonad may interrupt spermatogenesis by disrupting SC tight junctions(TJ) and it's proteins and hormone receptors. Pregnant Wistar rats were exposed to 50/100/200ppm CrVI through drinking water during embryonic days 9-14. On Postnatal day 120, testes were subjected to ion exchange chromatographic analysis and revealed increased level of CrIII in SCs and germ cells, serum and testicular interstitial fluid(TIF). Microscopic analyses showed seminiferous tubules atrophy and disruption of SC TJ, which also recorded decreased testosterone in TIF. mRNA and Protein expression analyses attested decreased level of Fshr, Ar, occludin and claudin-11 in SCs. Immunofluorescent detection revealed weak signal of TJ proteins. Taken together, we concluded that gestational exposure to CrVI interferes with the expression of SC TJ proteins due to attenuated expression of hormone receptors.

  11. {alpha} grain refining and metallurgical study of alloyed uranium, Sicral F1, used for fuel elements; Affinage du grain {alpha} et etude metallurgique de l'alliage d'uranium sicral F1 pour elements combustibles

    Energy Technology Data Exchange (ETDEWEB)

    Magnier, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1969-07-01

    This study was made to know more about grain refining in low alloyed uranium of composition not very different from SICRAL F 1. Alpha grain refining of fuel elements made of these alloys was studied after casting and quenching by the methods used for mass production. The author describes the effect: - of the metallurgical history before quenching: - casting - purity - rate of solidification - of quenching parameters: - annealing temperature before quenching - annealing time - quenching rate - of the composition of the alloy. For the graphite gas fuel elements of various dimensions, he suggests some modifications to give a better adaptation of fabrication to size. He describes the grain refining made during quenching and the {beta} -> {alpha} and {gamma} -> {alpha} transformation types. He proposes the use of a U-Fe-Si especially useful from the point of view of grain refining. (author) [French] Le but de l'etude est de determiner les facteurs metallurgiques favorables a l'affinage du grain {alpha} des alliages d'uranium a tres faibles teneurs en elements d'addition voisins du SICRAL F 1 au cours du cycle de fabrication et de trempe industrielle des elements combustibles nucleaires prepares avec ces alliages. L'auteur met en evidence l'influence: - de l'histoire metallurgique avant trempe: - coulee - teneur en impuretes - vitesse de solidification - des parametres de la trempe: - temperature de trempe - temps et maintien a cette temperature - vitesse de trempe - des variations de composition de l'alliage. Il envisage les modifications a apporter au cycle de fabrication du SICRAL F 1 de facon a l'adapter aux differentes geometries des elements combustibles des reacteurs de la filiere graphite-gaz. L'auteur presente a cette occasion les mecanismes de l'affinage du grain {alpha} par trempe dans les alliages d'uranium et les modes de transformation {beta} -> {alpha} et {gamma} -> {alpha} au cours de la trempe

  12. A family business: stem cell progeny join the niche to regulate homeostasis.

    Science.gov (United States)

    Hsu, Ya-Chieh; Fuchs, Elaine

    2012-01-23

    Stem cell niches, the discrete microenvironments in which the stem cells reside, play a dominant part in regulating stem cell activity and behaviours. Recent studies suggest that committed stem cell progeny become indispensable components of the niche in a wide range of stem cell systems. These unexpected niche inhabitants provide versatile feedback signals to their stem cell parents. Together with other heterologous cell types that constitute the niche, they contribute to the dynamics of the microenvironment. As progeny are often located in close proximity to stem cell niches, similar feedback regulations may be the underlying principles shared by different stem cell systems.

  13. Recombinant bovine heart mitochondrial F1-ATPase inhibitor protein: overproduction in Escherichia coli, purification, and structural studies.

    Science.gov (United States)

    Van Heeke, G; Deforce, L; Schnizer, R A; Shaw, R; Couton, J M; Shaw, G; Song, P S; Schuster, S M

    1993-09-28

    A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein was recovered from the bacterial cells by osmotic shock and was purified to near homogeneity in a single cation-exchange chromatography step. The recombinant inhibitor protein was shown to inhibit bovine mitochondrial F1-ATPase in a pH-dependent manner, as well as Saccharomyces cerevisiae mitochondrial F1-ATPase. Thorough analysis of the amino acid sequence revealed a potential coiled-coil structure for the C-terminal portion of the protein. Experimental evidence obtained by circular dichroism analyses supports this prediction and suggests F1I to be a highly stable, mainly alpha-helical protein which displays C-terminal alpha-helical coiled-coil intermolecular interaction.

  14. Mona F1: New pepper (Capsicum annuum L. hybrid in the Centre for Vegetable Crops

    Directory of Open Access Journals (Sweden)

    Cvikić Dejan

    2007-01-01

    Full Text Available The planted area various ways of pepper consumption (fresh or processed, make pepper one of the most important cultivars in vegetable breeding. In our country, up until now, the producers have usually grown varieties and domestic populations of pepper, while in more developed countries the usage of F1 hybrids is much more popular. The first pepper hybrids have been created in the Centre for Vegetable Crops by crossing new lines with male sterility gene ms-3 and selected genotypes from pepper collection. Created hybrids have higher yield, quality fruits and early ripening. This paper is the result of comparative trial in controlled conditions. Pepper varieties Župska rana, Zlatna medalja, Palanačka kapija and Duga bela, as well as new hybrid Mona F1 were the research matherial in order to observe the most important pepper traits.

  15. E2F1 and p53 Transcription Factors as Accessory Factors for Nucleotide Excision Repair

    Directory of Open Access Journals (Sweden)

    David G. Johnson

    2012-10-01

    Full Text Available Many of the biochemical details of nucleotide excision repair (NER have been established using purified proteins and DNA substrates. In cells however, DNA is tightly packaged around histones and other chromatin-associated proteins, which can be an obstacle to efficient repair. Several cooperating mechanisms enhance the efficiency of NER by altering chromatin structure. Interestingly, many of the players involved in modifying chromatin at sites of DNA damage were originally identified as regulators of transcription. These include ATP-dependent chromatin remodelers, histone modifying enzymes and several transcription factors. The p53 and E2F1 transcription factors are well known for their abilities to regulate gene expression in response to DNA damage. This review will highlight the underappreciated, transcription-independent functions of p53 and E2F1 in modifying chromatin structure in response to DNA damage to promote global NER.

  16. Proximity effects in superconducting triplet spin-valve F2/F1/S

    Energy Technology Data Exchange (ETDEWEB)

    Deminov, R.G., E-mail: Raphael.Deminov@kpfu.ru [Institute of Physics, Kazan Federal University, Kazan 420008 (Russian Federation); Tagirov, L.R. [Institute of Physics, Kazan Federal University, Kazan 420008 (Russian Federation); Institut für Physik, Universität Augsburg, Augsburg D-86159 (Germany); Gaifullin, R.R. [Institute of Physics, Kazan Federal University, Kazan 420008 (Russian Federation); Karminskaya, T.Yu.; Kupriyanov, M.Yu. [Skobeltsyn Institute of Nuclear Physics, Moscow State University, Moscow 119992 (Russian Federation); Fominov, Ya.V. [Landau Institute for Theoretical Physics RAS, Moscow 119334 (Russian Federation); Golubov, A.A. [Faculty of Science and Technology and MESA+ Institute of Nanotechnology, University of Twente, P.O. Box 217, Enschede 7500 AE (Netherlands)

    2015-01-01

    We investigate the critical temperature T{sub c} of F2/F1/S trilayers (Fi is a ferromagnetic metal and S is a singlet superconductor), where the long-range triplet superconducting component is generated at noncollinear magnetizations of the F layers. In this paper we demonstrate a possibility of the spin-valve effect mode selection (standard switching effect, the triplet spin-valve effect or reentrant T{sub c}(α) dependence) by the variation of the F2/F1 interface transparency. - Highlights: • T{sub c} of FFS trilayer as a function of angle between magnetizations is calculated. • T{sub c} of FFS structure for arbitrary FF interface transparencies γ{sub B} is calculated. • Possibility of the spin-valve effect mode selection by the variation of γ{sub B} is shown.

  17. Purification and Activity of Antibacterial Substances Derived from Soil Streptomyces sp.CaiF1

    Institute of Scientific and Technical Information of China (English)

    Hui YANG; Guixiang PENG; Jianmin ZENG; Zhiyuan TAN

    2012-01-01

    [Objective] This study aimed to separate and purify antibacterial sub- stances from soil Streptomyces sp. CaiF1, and to explore the activities of this sub- stance. [Method] The antibacterial substances were separated and purified by Ethyl acetate extraction, macroporous adsorptive resin, silica gel chromatography and preparative high performance liquid chromatography (HPLC), and powdery mildew were taken as the indicating bacterial to study their activities. [Result] Antibacterial substances were purified and the stability analysis of the extracts from Streptomyces CaiF1 fermentation broth showed very stable at pH 2.0-pH 10.0, 100 ~C and changed very little under UV treatment for 24 h. Inhibition rate of powdery mildew was 69.7%. [Conclusion] The purified antibacterial substances showed good stability, which provided theoretical foundation for their structural identifications and future ap- plications.

  18. Effects of Nonylphenol on Brain Gene Expression Profiles in F1 Generation Rats

    Institute of Scientific and Technical Information of China (English)

    YIN-YIN XIA; PING ZHANG; YANG WANG

    2008-01-01

    Objective To explore the effects of nonylphenol on brain gene expression profiles in F1 generation rats by microarray technique.Methods mRNA was extracted from the brain of 2-day old F1 generation male rats Whose F0 female generation was either exposed to nonylphenol or free from nonylphenol exposure,and then it was reversely transcribed to cDNA hbeled with cy5 and cy3 fluorescence.Subsequently,cDNA probes were hybridized to two BiostarR-40S cDNA gene chips and fluorescent signals of cy5 and cy3 were scanned and analyzed. Results Two genes were differentially down-regulated.Conclusion Nonylphenol may disturb the neurcendocrine function of male rats when administered perinatally.

  19. Oral vaccination with salmonella simultaneously expressing Yersinia pestis F1 and V antigens protects against bubonic and pneumonic plague.

    Science.gov (United States)

    Yang, Xinghong; Hinnebusch, B Joseph; Trunkle, Theresa; Bosio, Catharine M; Suo, Zhiyong; Tighe, Mike; Harmsen, Ann; Becker, Todd; Crist, Kathryn; Walters, Nancy; Avci, Recep; Pascual, David W

    2007-01-15

    The gut provides a large area for immunization enabling the development of mucosal and systemic Ab responses. To test whether the protective Ags to Yersinia pestis can be orally delivered, the Y. pestis caf1 operon, encoding the F1-Ag and virulence Ag (V-Ag) were cloned into attenuated Salmonella vaccine vectors. F1-Ag expression was controlled under a promoter from the caf1 operon; two different promoters (P), PtetA in pV3, PphoP in pV4, as well as a chimera of the two in pV55 were tested. F1-Ag was amply expressed; the chimera in the pV55 showed the best V-Ag expression. Oral immunization with Salmonella-F1 elicited elevated secretory (S)-IgA and serum IgG titers, and Salmonella-V-Ag(pV55) elicited much greater S-IgA and serum IgG Ab titers than Salmonella-V-Ag(pV3) or Salmonella-V-Ag(pV4). Hence, a new Salmonella vaccine, Salmonella-(F1+V)Ags, made with a single plasmid containing the caf1 operon and the chimeric promoter for V-Ag allowed the simultaneous expression of F1 capsule and V-Ag. Salmonella-(F1+V)Ags elicited elevated Ab titers similar to their monotypic derivatives. For bubonic plague, mice dosed with Salmonella-(F1+V)Ags and Salmonella-F1-Ag showed similar efficacy (>83% survival) against approximately 1000 LD(50) Y. pestis. For pneumonic plague, immunized mice required immunity to both F1- and V-Ags because the mice vaccinated with Salmonella-(F1+V)Ags protected against 100 LD(50) Y. pestis. These results show that a single Salmonella vaccine can deliver both F1- and V-Ags to effect both systemic and mucosal immune protection against Y. pestis.

  20. Direct analysis of airborne mite allergen (Der f1) in the residential atmosphere by chemifluorescent immunoassay using bioaerosol sampler.

    Science.gov (United States)

    Miyajima, Kumiko; Suzuki, Yurika; Miki, Daisuke; Arai, Moeka; Arakawa, Takahiro; Shimomura, Hiroji; Shiba, Kiyoko; Mitsubayashi, Kohji

    2014-06-01

    Dermatophagoides farinae allergen (Der f1) is one of the most important indoor allergens associated with allergic diseases in humans. Mite allergen Der f1 is usually associated with particles of high molecular weight; thus, Der f1 is generally present in settled dust. However, a small quantity of Der f1 can be aerosolized and become an airborne component. Until now, a reliable method of detecting airborne Der f1 has not been developed. The aim of this study was to develop a fiber-optic chemifluorescent immunoassay for the detection of airborne Der f1. In this method, the Der f1 concentration measured on the basis of the intensity of fluorescence amplified by an enzymatic reaction between the labeled enzyme by a detection antibody and a fluorescent substrate. The measured Der f1 concentration was in the range from 0.49 to 250 ng/ml and a similar range was found by enzyme-linked immunosorbent assay (ELISA). This method was proved to be highly sensitive to Der f1 compared with other airborne allergens. For the implementation of airborne allergen measurement in a residential environment, a bioaerosol sampler was constructed. The airborne allergen generated by a nebulizer was conveyed to a newly sampler we developed for collecting airborne Der f1. The sampler was composed of polymethyl methacrylate (PMMA) cells for gas/liquid phases and some porous membranes which were sandwiched in between the two phases. Der f1 in air was collected by the sampler and measured using the fiber-optic immunoassay system. The concentration of Der f1 in aerosolized standards was in the range from 0.125 to 2.0 mg/m(3) and the collection rate of the device was approximately 0.2%.

  1. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation

    DEFF Research Database (Denmark)

    Helin, K; Wu, C L; Fattaey, A R

    1993-01-01

    homolog of DP-1. Human DP-1 and E2F-1 associate both in vivo and in vitro, and this interaction leads to enhanced binding to E2F DNA-binding sites. The association of E2F-1 and DP-1 leads to cooperative activation of an E2F-responsive promoter. Finally, we demonstrate that E2F-1 and DP-1 association...

  2. Agraphia and acalculia after a left prefrontal (F1, F2) infarction.

    OpenAIRE

    Tohgi, H; Saitoh, K.; S. Takahashi(Kobe University, J-657-8501 Kobe, Japan); Takahashi, H; Utsugisawa, K; Yonezawa, H.; Hatano, K.; Sasaki, T.

    1995-01-01

    A patient presented with agraphia and acalculia associated with a left frontal (F1, F2) infarction. He made mainly phonological but also lexical errors in writing (syllabograms), but his ability to write kanji (morphograms) was relatively preserved. Although he could add and subtract numbers, he could neither multiply nor divide them because of a difficulty in retrieving the multiplication tables and calculation procedures. Positron emission tomography showed decreased cerebral blood flow and...

  3. VIIRS F1 "best" relative spectral response characterization by the government team

    Science.gov (United States)

    Moeller, Chris; McIntire, Jeff; Schwarting, Tom; Moyer, Dave

    2011-10-01

    The VIIRS Flight 1 (F1) instrument completed sensor level testing, including relative spectral response (RSR) characterization in 2009 and is moving forward towards a launch on the NPP platform late in 2011. As part of its mandate to produce analyses of F1 performance essentials, the VIIRS Government Team, consisting of NASA, Aerospace Corp., and MIT/Lincoln Lab elements, has produced an independent (from that of industry) analysis of F1 RSR. The test data used to derive RSR for all VIIRS spectral bands was collected in the TVAC environment using the Spectral Measurement Assembly (SpMA), a dual monochromator system with tungsten and ceramic glow bar sources. These spectrally contiguous measurements were analyzed by the Government Team to produce a complete in-band + out-of-band RSR for 21 of the 22 VIIRS bands (exception of the Day-Night Band). The analysis shows that VIIRS RSR was well measured in the pre-launch test program for all bands, although the measurement noise floor is high on the thermal imager band I5. The RSR contain expected detector to detector variation resulting from the VIIRS non-telecentric optical design, and out-of-band features are present in some bands; non-compliances on the integrated out-of-band spectral performance metric are noted in M15 and M16A,B bands and also for several VisNIR bands, though the VisNIR non-compliances were expected due to known scattering in the VisNIR integrated filter assembly. The Government Team "best" RSR have been released into the public domain for use by the science community in preparation for the post-launch era of VIIRS F1.

  4. Mitogenic Sonic hedgehog signaling drives E2F1-dependent lipogenesis in progenitor cells and Medulloblastoma

    OpenAIRE

    Bhatia, Bobby; Hsieh, Michael; Kenney, Anna Marie; Nahlé, Zaher

    2010-01-01

    Deregulation of the Rb/E2F tumor suppressor complex and aberrantion of Sonic hedgehog (Shh) signaling are documented across the spectrum of human malignancies. Exaggerated de novo lipid synthesis is also found in certain highly proliferative, aggressive tumors. Here, we show that in Shh-driven medulloblastomas, Rb is inactivated and E2F1 is up-regulated, promoting lipogenesis. Extensive lipid accumulation and elevated levels of the lipogenic enzyme FASN mark those tumors. In primary cerebella...

  5. Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.

    OpenAIRE

    Zylstra, G J; Wackett, L P; Gibson, D T

    1989-01-01

    Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroethylene degradation by the recombinant organism depended on the cell concentration and the concentration of trichloroethylene. A linear rate of trichloroethylene degradation was o...

  6. Effects of pedigree and exotic genetic inheritance on semen production traits of dairy bulls

    Directory of Open Access Journals (Sweden)

    Vijetha Bajjalli Thippeswamy

    2014-03-01

    Conclusions: It may be inferred that the “acceptable quality semen producing ability” decreased from grandsire through sire to male progeny and among the increasing exotic genetic levels of CB cattle, F1 bulls produced significantly higher “low grade ejaculates” that were unfit for cryopreservation.

  7. Studies of carcinogenicity of sodium chlorite in B6C3F1 mice.

    Science.gov (United States)

    Yokose, Y; Uchida, K; Nakae, D; Shiraiwa, K; Yamamoto, K; Konishi, Y

    1987-01-01

    The carcinogenic activities of sodium chlorite in B6C3F1 mice were examined. Sodium chlorite was given at concentrations of 0 (control), 0.025% (low dose), or 0.05% (high dose) in the drinking water of 150 female and 150 male mice for 80 weeks, after which time the animals were returned to distilled water without sodium chlorite. All mice were sacrificed 85 weeks from the beginning of the experiment. The incidence of tumor-bearing animals was 32% (control), 34% (low dose), and 26% (high dose) in female mice, and 46% (control), 57% (low dose), and 53% (high dose) in male mice. The types and incidence of neoplasms that occurred frequently in each group of both sexes were similar to those observed spontaneously in B6C3F1 mice. The incidence of lymphomas/leukemias in the high dose group of females (2%), however, was lower than that in the control group (15%). Furthermore, the incidence of pulmonary adenomas in the high dose group of males (12%) was higher than that in the control group (0%), but neither dose-related increases in the adenoma incidences nor increased incidences of the adenocarcinomas were observed. These results indicated no clear evidence of a carcinogenic potential of sodium chlorite in B6C3F1 mice. PMID:3447900

  8. Depigmenting Effect of Kojic Acid Esters in Hyperpigmented B16F1 Melanoma Cells

    Science.gov (United States)

    Lajis, Ahmad Firdaus B.; Hamid, Muhajir; Ariff, Arbakariya B.

    2012-01-01

    The depigmenting effect of kojic acid esters synthesized by the esterification of kojic acid using Rhizomucor miehei immobilized lipase was investigated in B16F1 melanoma cells. The depigmenting effect of kojic acid and kojic acid esters was evaluated by the inhibitory effect of melanin formation and tyrosinase activity on alpha-stimulating hormone- (α-MSH-) induced melanin synthesis in B16F1 melanoma cells. The cellular tyrosinase inhibitory effect of kojic acid monooleate, kojic acid monolaurate, and kojic acid monopalmitate was found similar to kojic acid at nontoxic doses ranging from 1.95 to 62.5 μg/mL. However, kojic acid monopalmitate gave slightly higher inhibition to melanin formation compared to other inhibitors at doses ranging from 15.63 to 62.5 μg/mL. Kojic acid and kojic acid esters also show antioxidant activity that will enhance the depigmenting effect. The cytotoxicity of kojic acid esters in B16F1 melanoma cells was significantly lower than kojic acid at high doses, ranging from 125 and 500 μg/mL. Since kojic acid esters have lower cytotoxic effect than kojic acid, it is suggested that kojic acid esters can be used as alternatives for a safe skin whitening agent and potential depigmenting agents to treat hyperpigmentation. PMID:23091364

  9. Properties of kojic acid and curcumin: Assay on cell B16-F1

    Science.gov (United States)

    Sugiharto, Ariff, Arbakariya; Ahmad, Syahida; Hamid, Muhajir

    2016-03-01

    Ultra violet (UV) exposure and oxidative stress are casually linked to skin disorders. They can increase melanin synthesis, proliferation of melanocytes, and hyperpigmentation. It is possible that antioxidants or inhibitors may have a beneficial effect on skin health to reduce hyperpigmentation. In the last few years, a huge number of natural herbal extracts have been tested to reduce hyperpigmentation. The objective of this study was to determine and to compare of kojic acid and curcumin properties to viability cell B16-F1. In this study, our data showed that the viability of cell B16-F1 was 63.91% for kojic acid and 64.12% for curcumin at concentration 100 µg/ml. Further investigation assay of antioxidant activities, indicated that IC50 for kojic acid is 63.8 µg/ml and curcumin is 16.05 µg/ml. Based on the data, kojic acid and curcumin have potential antioxidant properties to reduce hyperpigmentation with low toxicity effect in cell B16-F1.

  10. Quaternary structure of V1 and F1 ATPase: significance of structural homologies and diversities.

    Science.gov (United States)

    Svergun, D I; Konrad, S; Huss, M; Koch, M H; Wieczorek, H; Altendorf, K; Volkov, V V; Grüber, G

    1998-12-22

    The V1 ATPase from the tobacco hornworm Manduca sexta and the Escherichia coli F1 ATPase were characterized by small-angle X-ray scattering (SAXS). The radii of gyration (Rg) of the complexes were 6.2 +/- 0.1 and 4.7 +/- 0.02 nm, respectively. The shape of the M. sexta V1 ATPase was determined ab initio from the scattering data showing six masses, presumed to be the A and B subunits, arranged in an alternating manner about a 3-fold axis. A seventh mass with a length of about 11.0 nm extends perpendicularly to the center of the hexameric unit. This central mass is presumed to be the stalk that connects V1 with the membrane domain (V(O)) in the intact V1V(O)-ATPase. In comparison, the shape of the F1 ATPase from E. coli possesses a quasi-3-fold symmetry over the major part of the enzyme. The overall asymmetry of the structure is given by a stem, assumed to include the central stalk subunits. The features of the V1 and F1 ATPase reveal structural homologies and diversities of the key components of the complexes.

  11. Complementation of Escherichia coli unc mutant strains by chloroplast and cyanobacterial F1-ATPase subunits.

    Science.gov (United States)

    Lill, H; Burkovski, A; Altendorf, K; Junge, W; Engelbrecht, S

    1993-10-04

    The genes encoding the five subunits of the F1 portion of the ATPases from both spinach chloroplasts and the cyanobacterium Synechocystis sp. PCC 6803 were cloned into expression vectors and expressed in Escherichia coli. The recombinant subunits formed inclusion bodies within the cells. Each particular subunit was expressed in the respective unc mutant, each unable to grow on non-fermentable carbon sources. The following subunits restored growth under conditions of oxidative phosphorylation: alpha (both sources, cyanobacterial subunit more than spinach subunit), beta (cyanobacterial subunit only), delta (both spinach and Synechocystis), and epsilon (both sources), whereas no growth was achieved with the gamma subunits from both sources. Despite a high degree of sequence homology the large subunits alpha and beta of spinach and cyanobacterial F1 were not as effective in the substitution of their E. coli counterparts. On the other hand, the two smallest subunits of the E. coli ATPase could be more effectively replaced by their cyanobacterial or chloroplast counterparts, although the sequence identity or even similarity is very low. We attribute these findings to the different roles of these subunits in F1: The large alpha and beta subunits contribute to the catalytic centers of the enzyme, a function rendering them very sensitive to even minor changes. For the smaller delta and epsilon subunits it was sufficient to maintain a certain tertiary structure during evolution, with little emphasis on the conservation of particular amino acids.

  12. Depigmenting Effect of Kojic Acid Esters in Hyperpigmented B16F1 Melanoma Cells

    Directory of Open Access Journals (Sweden)

    Ahmad Firdaus B. Lajis

    2012-01-01

    Full Text Available The depigmenting effect of kojic acid esters synthesized by the esterification of kojic acid using Rhizomucor miehei immobilized lipase was investigated in B16F1 melanoma cells. The depigmenting effect of kojic acid and kojic acid esters was evaluated by the inhibitory effect of melanin formation and tyrosinase activity on alpha-stimulating hormone- (α-MSH- induced melanin synthesis in B16F1 melanoma cells. The cellular tyrosinase inhibitory effect of kojic acid monooleate, kojic acid monolaurate, and kojic acid monopalmitate was found similar to kojic acid at nontoxic doses ranging from 1.95 to 62.5 μg/mL. However, kojic acid monopalmitate gave slightly higher inhibition to melanin formation compared to other inhibitors at doses ranging from 15.63 to 62.5 μg/mL. Kojic acid and kojic acid esters also show antioxidant activity that will enhance the depigmenting effect. The cytotoxicity of kojic acid esters in B16F1 melanoma cells was significantly lower than kojic acid at high doses, ranging from 125 and 500 μg/mL. Since kojic acid esters have lower cytotoxic effect than kojic acid, it is suggested that kojic acid esters can be used as alternatives for a safe skin whitening agent and potential depigmenting agents to treat hyperpigmentation.

  13. Somatic development and embryo yield in crossbred F1 mice generated by different mating strategies

    Directory of Open Access Journals (Sweden)

    RITP. Batista

    Full Text Available The aim of this study was to evaluate different mating strategies among endogamic strains to create F1 populations of mice, minimising the effect of inbreeding depression on somatic development and embryo yield. Females from the strains Swiss, CBA and C57Bl/6 were divided in nine experimental mate arrangements. The total numbers of pups born alive per dam and somatic development, estimated by weighing and measuring the crown-rump length, were recorded. Superovulation response was evaluated in outbreed females. Litter size differed among endogamic dams, irrespective of the sire. Somatic development results suggest heterosis and imprinting phenomena, once a differential parental effect was demonstrated. There was no difference in corpora lutea, ova or embryos recovered (P > 0.05, but recovery and viability rates differ among F1 groups (P < 0.05. The association of dam prolificity with somatic development and superovulation response of the pups should be considered for experimental F1 populations establishment. The use of outbreed animals, however, did not reduce response variability to hormone treatment.

  14. Nanosized free-energy transducer F1-ATPase achieves 100% efficiency at finite time operation

    CERN Document Server

    Toyabe, Shoichi

    2012-01-01

    The free-energy transduction at 100% efficiency is not prohibited by thermodynamic laws. However, it is usually reached only at the quasi-static limit such as the macroscopic piston pulled or pushed at the infinitely slow velocity. If we operate the piston quickly, turbulence is inevitable and irreversible heat dissipates through the microscopic degrees of freedom. Here, we evaluated the work performed by the nano-sized biological free-energy transducer F1-ATPase by single-molecule experiments on the basis of nonequilibrium theory. We show that the F1-ATPase achieves a nearly 100% free-energy conversion efficiency even far from quasistatic process for both the mechanical-to-chemical and chemical-to-mechanical transductions. Such a high efficiency at a finite-time operation is not expected for macroscopic engines and highlights a remarkable property of the nano-sized engines working in the energy scale of k_{B}T. Some of the microscopic degrees of freedom may not be hidden but accessible to the F1-ATPase. Henc...

  15. Simultaneous F 0-F 1 modifications of Arabic for the improvement of natural-sounding

    Science.gov (United States)

    Ykhlef, F.; Bensebti, M.

    2013-03-01

    Pitch (F 0) modification is one of the most important problems in the area of speech synthesis. Several techniques have been developed in the literature to achieve this goal. The main restrictions of these techniques are in the modification range and the synthesised speech quality, intelligibility and naturalness. The control of formants in a spoken language can significantly improve the naturalness of the synthesised speech. This improvement is mainly dependent on the control of the first formant (F 1). Inspired by this observation, this article proposes a new approach that modifies both F 0 and F 1 of Arabic voiced sounds in order to improve the naturalness of the pitch shifted speech. The developed strategy takes a parallel processing approach, in which the analysis segments are decomposed into sub-bands in the wavelet domain, modified in the desired sub-band by using a resampling technique and reconstructed without affecting the remained sub-bands. Pitch marking and voicing detection are performed in the frequency decomposition step based on the comparison of the multi-level approximation and detail signals. The performance of the proposed technique is evaluated by listening tests and compared to the pitch synchronous overlap and add (PSOLA) technique in the third approximation level. Experimental results have shown that the manipulation in the wavelet domain of F 0 in conjunction with F 1 guarantees natural-sounding of the synthesised speech compared to the classical pitch modification technique. This improvement was appropriate for high pitch modifications.

  16. Bioleaching of heavy metals from a contaminated soil using indigenous Penicillium chrysogenum strain F1.

    Science.gov (United States)

    Deng, Xinhui; Chai, Liyuan; Yang, Zhihui; Tang, Chongjian; Tong, Haixia; Yuan, Pingfu

    2012-09-30

    Bioleaching of heavy metals from contaminated soil using Penicillium chrysogenum strain F1 was investigated. Batch experiments were performed to compare leaching efficiencies of heavy metals between one-step and two-step processes and to determine the transformation of heavy metal fractions before and after bioleaching. The results showed that two-step process had higher leaching efficiencies of heavy metals than one-step process. When the mass ratio of soil to culture medium containing P. chrysogenum strain F1 was 5% (w/v), 50%, 35%, 9% and 40% of Cd, Cu, Pb and Zn were removed in one-step process, respectively. The two-step process had higher removals of 63% Cd, 56% Cu, 14% Pb and 54% Zn as compared with one-step process. The results of the sequential extraction showed that the metals remaining in the soil were mainly bonded in stable fractions after bioleaching. The results of TEM and SEM showed that during bioleaching process, although the mycelium of P. chrysogenum was broken into fragments, no damage was obviously observed on the surface of the living cell except for thinner cell wall, smaller vacuoles and concentrated cytoplasm. The result implied that P. chrysogenum strain F1 can be used to remove heavy metals from polluted soil.

  17. F0F1-ATP-Synthase aus Escherichia coli: Untersuchung verschiedener Proteinsysteme mit ESR-Spektroskopie

    OpenAIRE

    Kraft, Gerhard

    1999-01-01

    Das Enzym F0F1-ATP-Synthase katalysiert die Phosphorylierung von ADP zu ATP unter Ausnutzung des durch die Atmungskette entstehenden Protonengradienten an Membranen. Hierbei pumpt der membranintegrale F0-Teil des Proteins Protonen durch die Membran und induziert die ATP-Synthese, welche auf dem peripheren, wasserlöslichen F1-Teil des Proteins (F1-ATPase) stattfindet. F0 besteht aus drei Proteinuntereinheiten der Stöchiometrie a b_2 c_9-12, während F1 aus fünf Untereinheiten der Stöchiometrie ...

  18. Evaluation of Dorset, Finnsheep, Romanov, Texel, and Montadale breeds of sheep: III. Wool characteristics of F1 ewes.

    Science.gov (United States)

    Lupton, C J; Freking, B A; Leymaster, K A

    2004-08-01

    An experiment was designed to evaluate the effects of five sire breeds (Dorset, Finnsheep, Romanov, Texel, and Montadale), two dam breeds (Composite III [CIII] and northwestern whiteface [WF]), and three shearing seasons (December, February, and April, corresponding to August, October, and December breeding seasons) and their interactions on wool and other characteristics of F1 ewes. Fleeces were collected and characterized from six 2-yr-old F1 ewes representing each of the 90 sire breed x dam breed x shearing season x year (three) subclasses. Characteristics measured objectively were grease and clean fleece weights, clean yield, mean fiber diameter and SD, and mean staple length and SD. Visual assessments of fleece color were also made. Data collected on the F1 ewes were analyzed using a mixed model analysis of variance procedure. The model included fixed effects of year of birth, sire breed, dam breed, shearing season, six two-way interactions, and the three-way interaction of sire breed x dam breed x shearing season. The random effect of individual sire within year of birth x sire breed was also fitted. Texel- and Montadale-sired ewes produced more clean wool (P wool (28.7 microm) (P wool (0.15 kg and 2.7 microm) than ewes from CIII dams (P wool (P wool production is opposite to that in conception rate (reported previously). Romanov-sired ewes produced the lowest percentage of white fleeces (62.6%), whereas Dorset-sired ewes produced the most (P Estimates of heritability were calculated for grease and clean fleece weights (0.36), percentage of clean yield (0.31), average fiber diameter and SD (0.86 and 0.42, respectively), and average staple length and SD (0.49 and 0.00, respectively). Although necessary for a thorough evaluation of these 10 types of crossbred ewes, it is estimated that wool income would only constitute a small portion (1 to 5%) of overall income from sheep of this type.

  19. Mapping of Hd6-f1 Gene on Rice Heading Date%水稻抽穗期基因 Hd6-f1的定位

    Institute of Scientific and Technical Information of China (English)

    周鹏飞; 柳絮; 张华; 宣宁; 李军; 杨永义; 李广贤; 姚方印; 刘开启

    2015-01-01

    本试验以明恢63(供体)/早熟豫6(受体)的BC4 F2分离群体为材料,采用BSA ( Bulked segrega-tion analysis)法,对控制水稻抽穗期分离的基因进行SSR分子标记定位。 BC4 F2出现抽穗期分离,早抽穗植株为253株,晚抽穗植株为657株,χ2=3.66<P0.05=3.84,符合1∶3的孟德尔分离比,表明F2群体的抽穗期分离受一对等位基因控制,晚抽穗性状为显性。以明恢63×早熟豫6的BC4 F2分离群体的253株隐性单株为定位群体,将该抽穗基因(暂时命名为Hd6-f1)定位在水稻第6染色体分子标记RM19771与RM527之间,遗传距离分别为0.2 cM和2.9 cM,与RM19780共分离。%Using the BC4F2 segregation populations of Minghui 63(donor) and Zaoshuyu 6(receptor) as materials, the SSR markers were located for the gene on rice heading date by the bulked segregation analysis (BSA).The BC4F2 population had 253 early heading plants and 657 late heading plants( χ2 =3.66f1 was mapped on chromosome 6 between SSR markers RM19771 and RM527 with the genetic distances as 0.2 cM and 2.9 cM respectively, and co-segregated with RM19780 .

  20. Desempenho agronômico de híbridos F1 de tomate de mesa Agronomic attributes of F1 fresh market tomato hybrids

    Directory of Open Access Journals (Sweden)

    Fabrício Franco B dos Santos

    2011-09-01

    Full Text Available Objetivou-se avaliar e selecionar híbridos experimentais F1 de tomate de mesa do grupo salada quanto ao desempenho agronômico em condições de campo. Conduziu-se o experimento na Estação Experimental da Nunhems do Brasil, em Paulinia-SP, de fevereiro a junho de 2008. Obtiveram-se híbridos experimentais entre dez linhagens do BAG de tomate da Nunhems. Utilizou-se o delineamento experimental blocos casualizados com 36 tratamentos, quatro repetições e dez plantas por parcela. Com base no agrupamento de médias pelo teste de Scott-Knott, os resultados do desempenho agronômico dos híbridos experimentais para sete características avaliadas mostraram grande variabilidade entre os genótipos para comprimento de fruto e largura de fruto, com quatro grupos de médias. Os híbridos mostraram boa variabilidade para produção de frutos por planta e altura de planta, com três agrupamentos cada e, para as características número de frutos por planta, número de pencas por planta e distância da primeira penca do solo, mostraram-se menos divergentes, com dois grupos de médias. HE-38 e HE-14, com valores heteróticos positivos, destacaram-se como os mais produtivos em relação ao híbrido-padrão Aplauso.The study aims to obtain and select F1 resistant hybrids of tomato for fresh market and evaluate their agronomic attributes in field conditions. The work was carried out at Nunhems Experimental Station, located in Paulínia, São Paulo State, Brazil, from February to June 2008. The experimental design was completely randomized blocks with 36 treatments, four replications and ten plants per plot. Based on the grouping of means by Scott-Knott test, the performance of hybrids for seven agronomic traits showed great variability among genotypes for fruit length and width with four groups of means. There was good variability for yield per plant, and plant height with three groups of means for each character. The traits fruit number per plant, cluster

  1. Comportamento de progênies F4 obtidas por cruzamentos de 'Icatu' com 'Catimor' Behavior of F4 progenies obtained from crossing of Icatu with Catimor

    Directory of Open Access Journals (Sweden)

    Gladyston Rodrigues Carvalho

    2009-02-01

    Full Text Available Conduziu-se este trabalho com o objetivo de avaliar progênies de cafeeiro resultantes do cruzamento entre 'Icatu' x 'Catimor', quanto à produtividade e vigor vegetativo e selecionar progênies superiores para avançar no programa de melhoramento dessa população. O delineamento utilizado foi em blocos casualizados com quatro repetições, 30 tratamentos (29 progênies do cruzamento mais a cultivar Rubi MG 1192, utilizada como testemunha. Para análise de variância dos dados adotou-se o esquema de parcela subdividida no tempo. Foram analisados a produção em sacas beneficiadas.ha-1 de seis colheitas 2001/2002 a 2006/2007 e o vigor vegetativo avaliado após a colheita 2006/2007. Os resultados obtidos permitem verificar a presença da variabilidade genética para produção entre as progênies avaliadas, fato confirmado pela alta estimativa da maioria das progênies estudadas, demonstraram que é possível selecionar progênies superiores na população estudada.The objective of this research was to evaluate coffee progenies resulted from the crossing of 'Icatu' with 'Catimor' in relation to their production and plant vigor and to select higher quality progenies to advance the breeding program of this population. For this purpose, a randomized complete block design was carried out with four replications and for the analysis of variance, the Split plot temporal arrangement was adopted. Twenty nine progenies plus the Rubi MG 1192 cultivar, used as a control, were evaluated. The yield of beans in bags.ha-1 of six harvests from 2001/2002 to 2006/2007 was evaluated and the plant vigor after the harvest was assessed. The results indicated that the evaluated progenies showed variability mainly for grain yield confirmed by heritability. This condition associated to high bean yields, which reached 62.0 bags.ha-1 and high plant vigor, showed the possibility of selecting outstanding progenies in the studied population.

  2. Tail asymptotics for the total progeny of the critical killed branching random walk

    CERN Document Server

    Aidekon, Elie

    2009-01-01

    We consider a branching random walk on $\\mathbb{R}$ with a killing barrier at zero. At criticality, the process becomes eventually extinct, and the total progeny $Z$ is therefore finite. We show that the tail distribution of $Z$ displays a typical behaviour in $(n\\ln^2(n))^{-1}$, which confirms the prediction of Addario-Berry and Broutin.

  3. Parental partners effects on progenies characteristics on hybridization within the Larix genus

    Energy Technology Data Exchange (ETDEWEB)

    Sindelar, J.; Frydl, J. [Forestry and Game Management Research Inst., Jiloviste (Czech Republic)

    1995-12-31

    Larch progenies of maternal clone No. 52-4-11 (Larix decidua Mill.) combined with the series of paternal clones of the same species and further of the Larix leptolepis Gord. and L. gmelini (Rupr.) Ledeb. species were evaluated on three research plots with larch (Larix sp.). Growth in height and thickness as well as stem forming were monitored at the age of 11 and 22 years. There was found out a significant share of variance falling on the action of mother, hence the existence of extra-nuclear heredity can be anticipated, particularly in interspecific hybrids. Comparing with the control in interspecific hybrids mostly luxuriant growth is displayed. On one of the studied plots there was found out the assumed growth depression in the quantitative characters of progenies originated from self-pollination. Some results of the research can be used as an auxiliary criterion in the selection of progenies and trees within the progenies for the establishment of seed orchards to produce hybrid seed of F2 generation. 24 refs, 6 figs, 6 tabs

  4. Dietary manipulation of the sow milk does not influence the lipid absorption capacity of the progeny

    DEFF Research Database (Denmark)

    Lauridsen, Charlotte; Hedemann, Mette Skou; Pierzynowski, Stefan

    2007-01-01

    A control diet without supplemental fat and four diets containing 8% of coconut oil, rapeseed oil, fish oil or sunflower oil were fed to lactating sows in order to investigate the lipid absorption capacity of their progeny in terms of pancreatic enzyme activity, hormonal regulation, and bile salt...

  5. Development of calibration facility for radon and its progenies at NIM (China).

    Science.gov (United States)

    Liang, J C; Zheng, P H; Yang, Z J; Liu, H R; Zhang, M; Li, Z S; Zhang, L; Guo, Q J

    2015-11-01

    Accurate measurement of radon and its progenies is the basis to control the radon dose and reduce the risk of lung cancer caused. The precise calibration of measuring instrument is an important part of the quality control of measurements of the concentration of radon and radon progenies. To establish Chinese national standards and realise reliable calibrations of measuring instrument for radon and its progenies, a radon chamber with regulation capability of environmental parameters, aerosol and radon concentrations was designed and constructed at National Institute of Metrology (NIM). The chamber has a total volume of ∼20 m(3) including an exposure volume of 12.44 m(3). The radon concentration can be controlled from 12 Bq m(-3) to the maximum of 232 kBq m(-3). The regulation range of temperature, relative humidity and aerosol are 0.66 -44.39°C, 16.4 -95 %RH and 10(2) -10(6) cm(-3), respectively. The main advantages of the NIM radon chamber with respect to maintaining a stable concentration and equilibrium factor of radon progenies in a wide range through automatic regulation and control of radon and aerosol are described.

  6. Cytogenetic Analysis of the Primary Amphidiploid Derived from Interspecific Hybridization in Cucumis and Its Selfed Progenies

    Institute of Scientific and Technical Information of China (English)

    CHEN Jin-feng; QIAN Chun-tao; J E Staub; LUO Xiang-dong; ZHUANG Fei-yun

    2005-01-01

    Studies on the reproduction and cytogenetic characterization of a primary amphidiploid Cucumis species C. hytivus Chen and Kirkbride (2n = 4x = 38) indicated that a more comprehensive cytogenetic analysis of this species and its first selfed progeny would increase its potential utility in cucumber improvement. With tendrils used as source materials for mitotic analysis, chromosome numbers in all selfed progenies were 2n = 38, confirming chromosomal stability in this synthetic amphidiploid species. Detailed meiotic processes were described by comparing the primary and the selfed amphidiploids.Meiotic abnormalities, such as chromosome lagging, unequal separation, chromosome multi-polarization and polyads were observed frequently in all amphidiploid plants except for the selfed no.8, in which meiosis was arrested prior to metaphase I. Generally, the frequency of multivalents was higher and the configurations were more complex in the selfed progenies, demonstrating a more extensive genetic exchange between cucumber and C. hystrix Chakr. Genome separation between cucumber and C. hystrix was observed through prophase ! to anaphase I in both generations of the amphidiploids. Consequently, in addition to n = 19, a new gamete with n = 7 was produced, which was confirmed by the chromosome counts 2n = 14 in the backcrossing progenies from cucumber × amphidiploid mating. Fertility varied among the selfed amphidiploid plants. The selfed plant no. 1 was found to have an improved fertility (e.g., pollen staining ability 40.8% and 25.6 seeds per fruit) and then was used as source germplasm in further introgression and gene exchange experiments.

  7. Effects of activity size distribution on dose conversion factor for radon progeny

    Energy Technology Data Exchange (ETDEWEB)

    Ishikawa, Tetsuo; Tokonami, Shinji; Yonehara, Hidenori; Fukutsu, Kumiko; Yamada, Yuji [National Inst. of Radiological Sciences, Chiba (Japan)

    2001-12-01

    To estimate dose from radon progeny, the effective dose per unit exposure to radon progeny (dose conversion factor, DCF) is needed. A dominant parameter related to DCF is the activity size distribution of radon progeny. In the present study, the DCF was calculated in the wide range of particle diameters (0.5-20 nm [AMTD] and 20-5,000 nm [AMAD]), using a dosimetric approach. The calculations were based on a computer program, LUDEP, which implements an ICRP66 respiratory tract model. The calculated results showed that the DCF is sensitive to particle size distribution. The DCFs calculated for reference conditions in mines and homes were 13.7 mSv WLM{sup -1} and 14.3 mSv WLM{sup -1}, respectively. These values were in good agreement with those reported in a few references. The DCF calculated in the present study is useful for the dose assessment of radon progeny in places that have different aerosol characteristics. (author)

  8. Frequency of Aneuploids in Progenies of Autotriploid Barley, Hordeum Vulgare L

    DEFF Research Database (Denmark)

    Sandfær, J.

    1979-01-01

    Chromosome counts of 863 progeny plants originating from 68 autotriploid barley plants revealed a considerable variation in chromosome numbers ranging from the diploid number (2n= 14) to 2n= 39. The most frequent groups were plants with 15 and 16 chromosomes each constituting about 27% of all pro...

  9. New strategy for evaluating grain cooking quality of progenies in dry bean breeding programs

    Directory of Open Access Journals (Sweden)

    Bruna Line Carvalho

    2017-04-01

    Full Text Available The methodology available for evaluating the cooking quality of dry beans is impractical for assessing a large number of progenies. The aims of this study were to propose a new strategy for evaluating cooking quality of grains and to estimate genetic and phenotypic parameters using a selection index. A total of 256 progenies of the 13thcycle of a recurrent selection program were evaluated at three locations for yield, grain type, and cooked grains. Samples of grains from each progeny were placing in a cooker and the percentage of cooked grains was assessed. The new strategy for evaluating cooking quality was efficient because it allowed a nine-fold increase in the number of progenies evaluated per unit time in comparison to available methods. The absence of association between grain yield and percentage of cooked grains or grain type indicated that it is possible to select high yielding lines with excellent grain aspect and good cooking properties using a selection index.

  10. {sup 222}Rn, {sup 220}Rn and their progeny concentrations in offices in Hong Kong

    Energy Technology Data Exchange (ETDEWEB)

    Yu, K.N. E-mail: appetery@city.edu.hk; Cheung, T.; Guan, Z.J.; Mui, B.W.N.; Ng, Y.T

    2000-04-01

    An active sampling system using charcoal canisters and an HPGe {gamma}-spectrometer was employed to survey {sup 222}Rn and {sup 220}Rn concentrations in 65 offices in Hong Kong, and a traditional method was used to record simultaneously the potential {alpha}-energy concentrations (PAEC) of {sup 222}Rn and {sup 220}Rn progeny at the same sites. The mean values of gas concentration, PAEC and equilibrium factor for {sup 222}Rn were 48{+-}32 Bq m{sup -3}, 5.2{+-}5.1 mWL and 0.38{+-}0.13, respectively, and the corresponding values for {sup 220}Rn were 14{+-}7 Bq m{sup -3}, 2.7{+-}2.1 mWL and 0.050{+-}0.016. These values were in general higher than those in dwellings in Hong Kong, which was due to the poorer fresh air exchange in offices. Factors affecting the concentrations of {sup 222}Rn, {sup 220}Rn and their progeny were also studied. The type of air conditioners and the indoor and outdoor temperature difference show some effects on {sup 222}Rn, {sup 220}Rn and progeny concentrations, while rainfall and relative humidity affect only the progeny concentrations.

  11. Long-term measurements of radon, thoron and their airborne progeny in 25 schools in Republic of Srpska.

    Science.gov (United States)

    Ćurguz, Z; Stojanovska, Z; Žunić, Z S; Kolarž, P; Ischikawa, T; Omori, Y; Mishra, R; Sapra, B K; Vaupotič, J; Ujić, P; Bossew, P

    2015-10-01

    This article reports results of the first investigations on indoor radon, thoron and their decay products concentration in 25 primary schools of Banja Luka, capital city of Republic Srpska. The measurements have been carried out in the period from May 2011 to April 2012 using 3 types of commercially available nuclear track detectors, named: long-term radon monitor (GAMMA 1)- for radon concentration measurements (C(Rn)); radon-thoron discriminative monitor (RADUET) for thoron concentration measurements (C(Tn)); while equilibrium equivalent radon concentration (EERC) and equilibrium equivalent thoron concentrations (EETC) measured by Direct Radon Progeny Sensors/Direct Thoron Progeny Sensors (DRPS/DTPS) were exposed in the period November 2011 to April 2012. In each school the detectors were deployed at 10 cm distance from the wall. The obtained geometric mean concentrations were C(Rn) = 99 Bq m(-3) and C(Tn) = 51 Bq m(-3) for radon and thoron gases respectively. Those for equilibrium equivalent radon concentration (EERC) and equilibrium equivalent thoron concentrations (EETC) were 11.2 Bq m(-3) and 0.4 Bq m(-3), respectively. The correlation analyses showed weak relation only between C(Rn) and C(Tn) as well as between C(Tn) and EETC. The influence of the school geographical locations and factors linked to buildings characteristic in relation to measured concentrations were tested. The geographical location and floor level significantly influence C(Rn) while C(Tn) depend only from building materials (ANOVA, p ≤ 0.05). The obtained geometric mean values of the equilibrium factors were 0.123 for radon and 0.008 for thoron.

  12. 牛、羊POU1F1基因的研究进展%The Advanced Progress of POU1F1 Gene in Bovine and Caprine

    Institute of Scientific and Technical Information of China (English)

    胡志焕

    2008-01-01

    POU1F1基因是 POU 基因家族的重要成员.POU1F1蛋白在哺乳动物胚胎分化和生长发育过程中起着关键作用.本文综述POU1F1基因的结构特征与功能,POU1F1蛋白的结构特征与作用方式,POU1F1蛋白的调控机制以及近年来对牛、羊POUlFl基因遗传变异及其与经济性状关系研究的最新进展.

  13. Variability and performance evaluation of introgressed Nigerian dura x Deli dura oil palm progenies.

    Science.gov (United States)

    Noh, A; Rafii, M Y; Mohd Din, A; Kushairi, A; Norziha, A; Rajanaidu, N; Latif, M A; Malek, M A

    2014-04-03

    Twelve introgressed oil palm (Elaeis guineensis) progenies of Nigerian dura x Deli dura were evaluated for bunch yield, yield attributes, bunch quality components and vegetative characters at the Malaysian Palm Oil Board Research Station, in Keratong, Pahang, Malaysia. Analysis of variance revealed significant to highly significant genotypic differences, indicating sufficient genetic variability among the progenies for bunch yield and its attributes, vegetative characters and bunch quality components, except fruit to bunch ratio. Fresh fruit bunch yield ranged from 167 kg·palm(-1)·year(-1) in PK1330 to 212 kg·palm(-1)·year(-1) in PK1351, with a mean yield of 192 kg·palm(-1)·year(-1). Among the progeny, PK1313 had the highest oil to bunch ratio (19.36%), due to its high mesocarp to fruit ratio, fruit to bunch ratio and low shell to fruit ratio. Among the progenies, PK1313 produced the highest oil yield of 31.4 kg·palm(-1)·year(-1), due to a high mesocarp to fruit ratio (61.2%) and a low shell to fruit ratio (30.7%), coupled with high fruit to bunch ratio (65.6%). PK1330 was found promising for selection, as it had desirable vegetative characters, including smaller petiole cross section (27.15 cm2), short rachis length (4.83 m), short palm height (1.85 m), and the lowest leaf number (164.6), as these vegetative characters are prerequisites for selecting palms for high density planting and high yield per hectare. The genetic variability among the progenies was found to be high, indicating ample scope for further breeding, followed by selection.

  14. Measurements of 222Rn and 220Rn with a Large Size Collector of Radon Progeny

    Institute of Scientific and Technical Information of China (English)

    Wu Qifan; Jia Wenyi; Fang Fang; Wang Jun; Cheng Jianping; Liu Guilin; Zhu Li

    2003-01-01

    Radon concentrations in high background radiation areas in the south are higher than those of others in China, especially 220Rn concentration is significantly high. Therefore, measurements of 222Rn and 220 Rn concentrations should be carried out there. This paper introduces a large size collector of radon progeny and its applications. The collector is a sheet of polyvinyl chloride fiber with electrostatics of (-500 V) - ( -700 V). Its size (60 mm in diameter) is larger than those of others (26 mm in diameter) that work with the same principle. The collector is more effective to adsorb radon progeny than most of others. The equipment of ZnS(Ag) Scintillation Counting System is available for large size collectors to detect radon progeny. Therefore, its sensitivity of measurement is higher than that of others.According to the different half lives of radon progeny, and based on both theory and experiments, a formula for discrimination and calculation of 222 Rn and 220Rn concentrations is deduced. The 222Rn and 220Rn concentrations were surveyed with electrostatic collectors of radon progeny on the campus of com-mercial school and some other areas in Hainan, southern China. Neither 222Rn nor 220Rn concentration was found significantly high. However, several faults underground were delineated. The collector is also used to study radon transportation. Results indicate that radon changes regularly with date when it has transported for a certain distance. Velocities of radon migration in the four media are quite different.Radon migrates more quickly in vertical tube than in the horizontal tube.

  15. 17 CFR 240.17f-1 - Requirements for reporting and inquiry with respect to missing, lost, counterfeit or stolen...

    Science.gov (United States)

    2010-04-01

    ... inquiry with respect to missing, lost, counterfeit or stolen securities. 240.17f-1 Section 240.17f-1... and inquiry with respect to missing, lost, counterfeit or stolen securities. (a) Definitions. For... not represented by an instrument and the transfer of which is registered upon books maintained...

  16. Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε.

    Science.gov (United States)

    Roy, Ankoor; Hutcheon, Marcus L; Duncan, Thomas M; Cingolani, Gino

    2012-10-01

    The bacterial ATP synthase (F(O)F(1)) of Escherichia coli has been the prominent model system for genetics, biochemical and more recently single-molecule studies on F-type ATP synthases. With 22 total polypeptide chains (total mass of ∼529 kDa), E. coli F(O)F(1) represents nature's smallest rotary motor, composed of a membrane-embedded proton transporter (F(O)) and a peripheral catalytic complex (F(1)). The ATPase activity of isolated F(1) is fully expressed by the α(3)β(3)γ 'core', whereas single δ and ε subunits are required for structural and functional coupling of E. coli F(1) to F(O). In contrast to mitochondrial F(1)-ATPases that have been determined to atomic resolution, the bacterial homologues have proven very difficult to crystallize. In this paper, we describe a biochemical strategy that led us to improve the crystallogenesis of the E. coli F(1)-ATPase catalytic core. Destabilizing the compact conformation of ε's C-terminal domain with a phosphomimetic mutation (εS65D) dramatically increased crystallization success and reproducibility, yielding crystals of E. coli F(1) that diffract to ∼3.15 Å resolution.

  17. The Role of a Novel Nucleolar Protein in Regulation of E2F1 in Breast Cancer

    Science.gov (United States)

    2009-09-01

    copresence of RRP1B and E2F1 on specific E2F1 targets (see below), We would now like to discuss progress in the research program. Task 1...A similar assay was performed identically except that cells were grown in media containing 2% fetal bovine serum. Real time and semiquantitative

  18. Positive and negative regulation of cell proliferation by E2F-1: influence of protein level and human papillomavirus oncoproteins

    DEFF Research Database (Denmark)

    Melillo, R M; Helin, K; Lowy, D R;

    1994-01-01

    E2F-1 is a member of a family of transcription factors implicated in the activation of genes required for the progression through the S phase of the cell cycle. We have examined the biological activities of E2F-1 with short-term colony-forming assays and long-term immortalization assays. High lev...

  19. Response of PS Ⅱ photochemistry activity of F1 progeny of transgenic tobacco seedlings with 2-Cys Prx to drought stress%转2-Cys Prx基因烟草F1幼苗叶片光系统Ⅱ光化学活性对干旱胁迫的响应

    Institute of Scientific and Technical Information of China (English)

    胡举伟; 张会慧; 逄好胜; 孙广玉

    2014-01-01

    以转2-Cys Prx基因烟草(龙江911)为材料,测定和分析了干旱胁迫下转基因烟草的光化学活性.结果表明:干旱胁迫下,转2-Cys Prx基因烟草叶片OJIP曲线上K点和J点的相对荧光强度增加量明显低于非转基因烟草,而2 ms时有活性反应中心的开放程度(Ψo)明显高于非转基因烟草;增强2-Cys Prx基因的相对表达,增加了干旱胁迫下烟草叶片PS Ⅱ电子供体侧OEC的电荷分离能力和受体侧QA向QB的电子传递能力;转2-Cys Prx基因烟草叶片在干旱胁迫下,吸收光能用于QA-以后的电子传递的能量比例(φEo)明显大于非转基因烟草,而非光化学猝灭的最大量子产额(φDo).2-Cys Prx基因的表达可以提高烟草幼苗叶片的光化学活性,并改变光能的分配来增强其抗旱能力.

  20. y=f(x+1)与y=f-1(x+1)互为反函数吗?

    Institute of Scientific and Technical Information of China (English)

    邓先会; 鲁荣光

    2002-01-01

    @@ 从图象上看y=f(x+1)与y=f-1(x+1)的图象是分别将y=f(x)与y=f-1(x)的图象向左平移一个单位所得,因y=f(x)与y=f-1(x)图象关于y=x对称,将y=x向左平移一个单位得y=x+1,所以函数y=f(x+1)与y=f-1(x+1)的图象关于y=x+1对称,因而y=f(x+1)与y=f-1(x+1)不一定互为反函数.

  1. Lack of ability of trypsin-treated mitochondrial F1-ATPase to bind the oligomycin-sensitivity conferring protein (OSCP).

    Science.gov (United States)

    Hundal, T; Norling, B; Ernster, L

    1983-10-03

    Soluble beef-heart mitochondrial F1-ATPase modified in its alpha-subunit by mild trypsin treatment (alpha'-F1) can no longer bind oligomycin-sensitivity conferring protein (OSCP) but is still capable of binding to F1-depleted submitochondrial particles, giving rise to a maximally oligomycin-sensitive ATPase, provided the particles contain their native complement of OSCP. When OSCP is removed from the particles, alpha'-F1 can still bind to the particles, but added OSCP induces only a low degree of oligomycin sensitivity. The possible role of OSCP in the functional coupling of the catalytic (F1) and H+-translocating (Fo) moieties of mitochondrial ATPase is discussed. The results suggest a functional similarity between the OSCP component of mitochondrial ATPase and the delta-subunit of E. coli ATPase, which is in accordance with the structural homology recently found to exist between the two polypeptides.

  2. 紫贻贝和厚壳贻贝杂交及F1代杂交优势初探%A primary study on hybridization of Mytilus galloprovincialis, Mytilus coruscus, heterosis of F1 generation

    Institute of Scientific and Technical Information of China (English)

    常抗美; 刘慧慧; 李家乐; 沈玉帮

    2008-01-01

    2005-2007年对紫贻贝(Mytilus gallo provincialis)和厚壳贻贝(Mytilus coruscus)种间进行了杂交生产性试验,通过亲贝强化培育、确认雌雄亲贝、改革育苗水体交换方法、投喂混合单细胞藻类、流水培养附着稚贝等技术,获得F1代.结果表明,正交F1代幼虫的成活率明显高于反交F1代和厚壳贻贝,在幼虫培育前期与紫贻贝的成活率相当,但随着幼虫的发育有高于紫贻贝的趋势.正交F1代幼虫的壳长和壳高在培育阶段的前期生长与紫贻贝相当,低于厚壳贻贝,高于反交F1代;但后期的生长明显增快,并高于反交F1代和其他两种贻贝.各试验组贻贝海区养殖的成活率在90%以上,正交F1代最高,显著高于反交F1代.各试验组生长特性,正交F1代紫贻贝厚壳贻贝反交F1代.其中正交F1代获附着稚贝3.77×109 ind.正交F1除在孵化率上低于紫贻贝、厚壳贻贝外,在壳长、壳高、成活率等方面指标具有一定杂交优势,而反交F1代的杂交优势不明显.

  3. Surface Plasmon Resonance Analysis of Histidine-Tagged F1-ATPase Surface Adsorption

    Science.gov (United States)

    Tucker, Jenifer K.; Richter, Mark L.; Berrie, Cindy L.

    2015-11-01

    Studies of the rotational activity of the enzymatic core (α3β3γ) of the F1-ATPase motor protein have relied on binding the enzyme to NTA-coated glass surfaces via polyhistidine tags engineered into the C-termini of each of the three α or β subunits. Those studies revealed the rotational motion of the central γ subunit by monitoring the motion of attached micron-long actin filaments or spherical nanoparticles. However, only a small percentage of the attached filaments or particles were observed to rotate, likely due, at least in part, to non-uniform surface attachment of the motor proteins. In this study, we have applied surface plasmon resonance to monitor the kinetics and affinity of binding of the His-tagged motor protein to NTA-coated gold sensor surfaces. The binding data, when fit to a heterogeneous binding model, exhibit two sets of adsorption-desorption rate constants with two dissociation constants of 4.0 × 10-9 M and 8.6 × 10-11 M for 6His-α3β3γ binding to the nickel ion-activated NTA surface. The data are consistent with mixed attachment of the protein via two (bimodal) and three (trimodal) NTA/Ni2+-His-tag interactions, respectively, with the less stable bimodal interaction dominating. The results provide a partial explanation for the low number of surface-attached F1 motors previously observed in rotation studies and suggest alternative approaches to uniform F1 motor surface attachment for future fabrication of motor-based nanobiodevices and materials.

  4. Red nucleus and rubrospinal tract disorganization in the absence of Pou4f1

    Science.gov (United States)

    Martinez-Lopez, Jesus E.; Moreno-Bravo, Juan A.; Madrigal, M. Pilar; Martinez, Salvador; Puelles, Eduardo

    2015-01-01

    The red nucleus (RN) is a neuronal population that plays an important role in forelimb motor control and locomotion. Histologically it is subdivided into two subpopulations, the parvocellular RN (pRN) located in the diencephalon and the magnocellular RN (mRN) in the mesencephalon. The RN integrates signals from motor cortex and cerebellum and projects to spinal cord interneurons and motor neurons through the rubrospinal tract (RST). Pou4f1 is a transcription factor highly expressed in this nucleus that has been related to its specification. Here we profoundly analyzed consequences of Pou4f1 loss-of-function in development, maturation and axonal projection of the RN. Surprisingly, RN neurons are specified and maintained in the mutant, no cell death was detected. Nevertheless, the nucleus appeared disorganized with a strong delay in radial migration and with a wider neuronal distribution; the neurons did not form a compacted population as they do in controls, Robo1 and Slit2 were miss-expressed. Cplx1 and Npas1, expressed in the RN, are transcription factors involved in neurotransmitter release, neuronal maturation and motor function processes among others. In our mutant mice, both transcription factors are lost, suggesting an abnormal maturation of the RN. The resulting altered nucleus occupied a wider territory. Finally, we examined RST development and found that the RN neurons were able to project to the spinal cord but their axons appeared defasciculated. These data suggest that Pou4f1 is necessary for the maturation of RN neurons but not for their specification and maintenance. PMID:25698939

  5. None of the Rotor Residues of F1-ATPase Are Essential for Torque Generation

    Science.gov (United States)

    Chiwata, Ryohei; Kohori, Ayako; Kawakami, Tomonari; Shiroguchi, Katsuyuki; Furuike, Shou; Adachi, Kengo; Sutoh, Kazuo; Yoshida, Masasuke; Kinosita, Kazuhiko

    2014-01-01

    F1-ATPase is a powerful rotary molecular motor that can rotate an object several hundred times as large as the motor itself against the viscous friction of water. Forced reverse rotation has been shown to lead to ATP synthesis, implying that the mechanical work against the motor’s high torque can be converted into the chemical energy of ATP. The minimal composition of the motor protein is α3β3γ subunits, where the central rotor subunit γ turns inside a stator cylinder made of alternately arranged α3β3 subunits using the energy derived from ATP hydrolysis. The rotor consists of an axle, a coiled coil of the amino- and carboxyl-terminal α-helices of γ, which deeply penetrates the stator cylinder, and a globular protrusion that juts out from the stator. Previous work has shown that, for a thermophilic F1, significant portions of the axle can be truncated and the motor still rotates a submicron sized bead duplex, indicating generation of up to half the wild-type (WT) torque. Here, we inquire if any specific interactions between the stator and the rest of the rotor are needed for the generation of a sizable torque. We truncated the protruding portion of the rotor and replaced part of the remaining axle residues such that every residue of the rotor has been deleted or replaced in this or previous truncation mutants. This protrusionless construct showed an unloaded rotary speed about a quarter of the WT, and generated one-third to one-half of the WT torque. No residue-specific interactions are needed for this much performance. F1 is so designed that the basic rotor-stator interactions for torque generation and control of catalysis rely solely upon the shape and size of the rotor at very low resolution. Additional tailored interactions augment the torque to allow ATP synthesis under physiological conditions. PMID:24853745

  6. Binding and hydrolysis of TNP-ATP by Escherichia coli F1-ATPase.

    Science.gov (United States)

    Weber, J; Senior, A E

    1996-02-16

    It had previously been suggested that Vmax hydrolysis rate of 2', 3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP) by F1-ATPase required filling of only two catalytic sites on the enzyme (Grubmeyer, C., and Penefsky, H. S. (1981) J. Biol. Chem. 256, 3718-3727), whereas recently it was shown that Vmax rate of ATP hydrolysis requires that all three catalytic sites are filled (Weber, J., Wilke-Mounts, S., Lee, R. S. F., Grell, E., and Senior, A. E. (1993) J. Biol. Chem. 268, 20126-20133). To resolve this apparent discrepancy, we measured equilibrium binding and hydrolysis of MgTNP-ATP under identical conditions, using betaY331W mutant Escherichia coli F1-ATPase, in which the genetically engineered tryptophan provides a direct fluorescent probe of catalytic site occupancy. We found that MgTNP-ATP hydrolysis at Vmax rate did require filling of all three catalytic sites, but in contrast to the situation with MgATP, "bisite hydrolysis" of MgTNP-ATP amounted to a substantial fraction (approximately 40%) of Vmax. Binding of MgTNP-ATP to the three catalytic sites showed strong binding cooperativity (Kd1 e. in presence of EDTA) bound to all three catalytic sites with lower affinity but was not hydrolyzed. These data emphasize that the presence of Mg2+ is critical for cooperativity of substrate binding, formation of the very high affinity first catalytic site, and hydrolytic activity in F1-ATPases and that these three properties are strongly correlated.

  7. Classifying genotype F of hepatitis B virus into F1 and F2 subtypes

    Institute of Scientific and Technical Information of China (English)

    Hideaki Kato; Takanobu Kato; Yuzo Miyakawa; Masashi Mizokami; Kei Fujiwara; Robert G. Gish; Hiroshi Sakugawa; Hiroshi Yoshizawa; Fuminaka Sugauchi; Etsuro Orito; Ryuzo Ueda; Yasuhito Tanaka

    2005-01-01

    AIM: To explore the propriety of providing hepatitis B virus(HBV) genotypes F and H with two distinct genotypes.METHODS: Eleven HBV isolates of genotype F (HBV/F)were recovered from patients living in San Francisco,Japan, Panama, and Venezuela, and their full-length sequences were determined. Phylogenetic analysis was carried out among them along with HBV isolates previously reported.RESULTS: Seven of them clustered with reported HBV/F isolates in the phylogenetic tree constructed on the entire genomic sequence. The remaining four flocked on another branch along with three HBV isolates formerly reported as genotype H. These seven HBV isolates, including the four in this study and the three reported, had a sequence divergence of 7.3-9.5% from the other HBV/F isolates,and differed by > 13.7% from HBV isolates of the other six genotypes (A-E and G). Based on a marked genomic divergence, falling just short of >8% separating the seven genotypes, these seven HBV/F isolates were classified into F2 subtype and the former seven into F1 subtype provisionally. In a pairwise comparison of the S-gene sequences among the 7 HBV/F2 isolates and against 47HBV/F1 isolates as well as 136 representing the other six genotypes (A-E and G), two clusters separated by distinct genetic distances emerged.CONCLUSION: Based on these analyses, classifying HBV/F isolates into two subtypes (F1 and F2) would be more appropriate than providing them with two distinct genotypes (F and H).

  8. New orbit recalculations of comet C/1890 F