WorldWideScience

Sample records for extracurricular activity involvement

  1. Adolescent Involvement in Extracurricular Activities: Influences on Leadership Skills

    Science.gov (United States)

    Hancock, Donna; Dyk, Patricia Hyjer; Jones, Kenneth

    2012-01-01

    Study examined adolescents' participation in sports, school, and community extracurricular activities to assess the influence of different involvement roles and adult support on leadership skills. The study found that males and females who perceived their adult support more positively had more positive perceptions of their leadership skills.…

  2. Extracurricular Activity Involvement and Adolescent Self-Esteem

    Science.gov (United States)

    Kort-Butler, Lisa A.

    2012-01-01

    Structured extracurricular activity participation has been linked to self-esteem and other indicators of positive youth development. This article describes the theoretical basis for this relationship, centering on extracurricular activities as a location for identity development. A summary of the empirical evidence points to the importance of…

  3. Extracurricular Activity Involvement and Adolescent Self-Esteem

    Science.gov (United States)

    Kort-Butler, Lisa A.

    2012-01-01

    Structured extracurricular activity participation has been linked to self-esteem and other indicators of positive youth development. This article describes the theoretical basis for this relationship, centering on extracurricular activities as a location for identity development. A summary of the empirical evidence points to the importance of…

  4. A Study of Lipscomb University Students' Internet Use and Involvement in Extracurricular Activities

    Science.gov (United States)

    Smith, Samuel Aarron

    2010-01-01

    The purpose of this study was to analyze Lipscomb University students' Internet use and involvement in extracurricular activities. A survey of students at Lipscomb University was conducted. As confirmed by the data the research was able to determine that the type of extracurricular activity a student participates in most often is related to the…

  5. THE INFLUENCE OF STUDENTS’ INVOLVEMENT IN EXTRACURRICULAR ACTIVITIES ON THE DEVELOPMENT OF SKILLS AND COMPETENCIES

    Directory of Open Access Journals (Sweden)

    Oana DUMITRASCU

    2015-11-01

    Full Text Available The paper aims to demonstrate the influence of students’ involvement in extracurricular activities on the development of skills and competencies by means of the factorial analysis. This research is untertaken by means of the questionnaire method, where 728 respondents from five Romanian universities where questionned. The data is analysed through the statistical programme SPSS. Due to the factorial analysis certain influences of the extracurricular activities on the organisational skills, social competencies, communication skills and integration ability are highlighted.

  6. Beyond participation: the association between school extracurricular activities and involvement in violence across generations of immigration.

    Science.gov (United States)

    Jiang, Xin; Peterson, Ruth D

    2012-03-01

    Participation in extracurricular activities is purported to protect the broad spectrum of youth from a host of behavioral risks. Yet, empirical research on the extent to which this assumption holds for involvement in violence by immigrant youth is limited. Thus, using data for 13,236 (51.8% female) adolescents from the National Longitudinal Study of Adolescent Health, this study explores how the relationship between extracurricular activities and youth violence varies by type of extracurricular activity profile (sports alone, non-sports alone, and a combination of sports and non-sports) and by generations of immigration (first, second, and third-plus). The sample is composed of 9.3% (n = 1,233) first-generation youth, 15.7% (n = 2,080) second generation, and 74.9% (n = 9,923) third-plus generation. The results reveal that adolescents from the third-plus generation (i.e., non-immigrant youth) who participate in non-sports alone or sports plus non-sports have lower odds of involvement in violence than adolescents from the same generation who do not participate in extracurricular activities. However, for first- and second-generation adolescents, participation in extracurricular activities is associated with higher rather than lower odds of violence compared to their non-participating counterparts. These findings challenge the viewpoint that participation in mainstream extracurricular activities as afforded by US schools is equally beneficial for all youth. They also call for additional research that explores why immigrant youth are less likely than non-immigrant youth to gain violence-reducing benefits when they participate in extracurricular activities.

  7. Extracurricular Activity and Parental Involvement Predict Positive Outcomes in Elementary School Children

    Science.gov (United States)

    Lagace-Seguin, Daniel G.; Case, Emily

    2010-01-01

    The main goal of this study was to explore if parental involvement and extracurricular activity participation could predict well-being and academic competence in elementary school children. Seventy-two children (mean age = 10.9 years, SD = 0.85) and their parents participated. Results revealed that parental pressure and support, when paired with…

  8. School-Based Extracurricular Activity Involvement and Adolescent Self-Esteem: A Growth-Curve Analysis

    Science.gov (United States)

    Kort-Butler, Lisa A.; Hagewen, Kellie J.

    2011-01-01

    Research on adolescent self-esteem indicates that adolescence is a time in which individuals experience important changes in their physical, cognitive, and social identities. Prior research suggests that there is a positive relationship between an adolescent's participation in structured extracurricular activities and well-being in a variety of…

  9. School-Based Extracurricular Activity Involvement and Adolescent Self-Esteem: A Growth-Curve Analysis

    Science.gov (United States)

    Kort-Butler, Lisa A.; Hagewen, Kellie J.

    2011-01-01

    Research on adolescent self-esteem indicates that adolescence is a time in which individuals experience important changes in their physical, cognitive, and social identities. Prior research suggests that there is a positive relationship between an adolescent's participation in structured extracurricular activities and well-being in a variety of…

  10. School-based extracurricular activity involvement and adolescent self-esteem: a growth-curve analysis.

    Science.gov (United States)

    Kort-Butler, Lisa A; Hagewen, Kellie J

    2011-05-01

    Research on adolescent self-esteem indicates that adolescence is a time in which individuals experience important changes in their physical, cognitive, and social identities. Prior research suggests that there is a positive relationship between an adolescent's participation in structured extracurricular activities and well-being in a variety of domains, and some research indicates that these relationships may be dependent on the type of activities in which adolescents participate. Building on previous research, a growth-curve analysis was utilized to examine self-esteem trajectories from adolescence (age 14) to young adulthood (age 26). Using 3 waves of data from National Longitudinal Study of Adolescent Health (n = 5,399; 47.8% male), the analysis estimated a hierarchical growth-curve model emphasizing the effects of age and type of school-based extracurricular activity portfolio, including sports and school clubs, on self-esteem. The results indicated that age had a linear relationship with self-esteem over time. Changes in both the initial level of self-esteem and the growth of self-esteem over time were significantly influenced by the type of extracurricular activity portfolio. The findings were consistent across race and sex. The results support the utility of examining the longitudinal impact of portfolio type on well-being outcomes.

  11. An Initial Investigation of Sexual Minority Youth Involvement in School-Based Extracurricular Activities

    OpenAIRE

    Toomey, Russell B.; Russell, Stephen T.

    2012-01-01

    Sexual minority youth are at risk for negative school-based experiences and poor academic outcomes. Yet, little is known about their experiences in positive school-based contexts. Using the National Longitudinal Study of Adolescent Health (1,214 sexual minority and 11,427 heterosexual participants), this study compared participation rates in, predictors of, and outcomes associated with three types of school-based extracurricular activities - sports, arts, and school clubs - by sexual orientat...

  12. Beyond the Classroom: Involving Students with Disabilities in Extracurricular Activities at Levy Middle School.

    Science.gov (United States)

    Walker, Pam; And Others

    Six students in a special education classroom at Levy Middle School (Syracuse, New York) became involved in a variety of after-school activities with nondisabled students. The students participated in the school computer club, cross-country skiing, volleyball, stage crew, intramural basketball, the Spanish Club, and after-school programs at two…

  13. Extracurricular activities in young applicants' résumés: what are the motives behind their involvement?

    Science.gov (United States)

    Roulin, Nicolas; Bangerter, Adrian

    2013-01-01

    Applicants use résumés to demonstrate their knowledge, skills, abilities, and other personal characteristics (KSAOs) to recruiters, through education and job-related or non-job-related experiences. But research suggests that the situation for young applicants is especially competitive, since they increasingly enter the labour market with similar educational credentials and limited job-related experience. They may thus use non-job-related experiences, such as participation in extracurricular activities (ECAs) during their studies, to demonstrate KSAOs to recruiters, but also to add distinction and value to their credentials. ECAs may therefore become more important in the selection of young applicants. Yet few studies have undertaken a comprehensive and systematic analysis of the relationships students have with these activities. The purpose of this study was to investigate to what extent students' involvement in ECAs is due to internal (e.g., passion) or external (e.g., résumé-building) motives, and what factors influence these motives. Results from a study with 197 students suggest that students engage in ECAs mainly out of internal motives. But external motives are stronger for activities started closer to entering the labour market, for students active in associative or volunteering activities (as compared to sports or artistic activities), and for students holding leadership positions in their activities. Our results suggest that labour market pressure may be a key component of applicants' involvement in ECAs. Also, organizations and recruiters may want to consider that students tend not to engage in ECAs purely out of internal motives, but also to add value to their credentials and match employers' expectations. The authors thank Anna Ambrosetti for her help with the data collection.

  14. Keeping Adolescents Busy with Extracurricular Activities

    Science.gov (United States)

    Kao, Tsui-Sui Annie; Salerno, Jennifer

    2014-01-01

    Adolescent participation in academic/extracurricular activities is related to fewer diagnoses of sexually transmitted infections during adolescence. The role parents play in motivating participation in such activities is unclear. This mixed-methods study explored parental influences on academic/extracurricular activity participation, and the…

  15. Effects of Extracurricular Activities on Postsecondary Completion for Students with Disabilities

    Science.gov (United States)

    Palmer, Ashley N.; Elliott, William, III; Cheatham, Gregory A.

    2017-01-01

    The authors focused on participation in extracurricular activities as a way of improving the educational outcomes of children with disabilities. Regarding students in the general population, adolescent involvement in extracurricular activities has been shown to have a positive association with school involvement and adolescent self-esteem,…

  16. Effects of Extracurricular Activities on Postsecondary Completion for Students with Disabilities

    Science.gov (United States)

    Palmer, Ashley N.; Elliott, William, III; Cheatham, Gregory A.

    2017-01-01

    The authors focused on participation in extracurricular activities as a way of improving the educational outcomes of children with disabilities. Regarding students in the general population, adolescent involvement in extracurricular activities has been shown to have a positive association with school involvement and adolescent self-esteem,…

  17. Participation in Extracurricular Physical Activity Programs at Middle Schools.

    Science.gov (United States)

    Powers, Holly S.; Conway, Terry L.; McKenzie, Thomas L.; Sallis, James F.; Marshall, Simon J.

    2002-01-01

    Evaluated student participation in structured, on-campus extracurricular activities at 24 middle schools. Overall, boys and girls participated at similar rates (except for intramurals). All schools offered multiple extracurricular activity programs, but due to low participation rates, the amount of physical activity obtained was minimal.…

  18. Burnout and its association with extracurricular activities among medical students in Saudi Arabia.

    Science.gov (United States)

    Almalki, Sami A; Almojali, Abdullah I; Alothman, Ali S; Masuadi, Emad M; Alaqeel, Meshal K

    2017-04-26

    To assess levels of burnout in medical students, and to explore the influence of extracurricular activities on burnout at a medical school in Saudi Arabia. This cross-sectional study was conducted with first to fourth year medical students at King Saud bin Abdulaziz University for Health Sciences (KSAU-HS) in Riyadh, Saudi Arabia. Socio-demographic, burnout level (the Maslach Burnout Inventory-Student Survey, MBI-SS) and participation in extracurricular activities data were collected using a self-administered questionnaire. Statistical analyses were performed using the Pearson's chi-square test and binary logistic regression. From the 306 medical students approached, 249 (81.4%) completed the questionnaire. The level of high burnout was 67.1% (n=167). The study revealed that the majority (62.3%, n=155) of students had high levels of cynicism, 58.6% (n=146) had high levels of emotional exhaustion, and 60.2% (n=150) had low levels of professional efficacy. Most of the students (73.5%, n=183) participated in extracurricular activities, and 112 (45%) students were organizers of extracurricular activities. No significant association was found between burnout levels and the frequency of involvement in extracurricular activities (χ(2)=2.2, df=2, p=0.333). However, students who were organizing extracurricular activities were less likely to have low professional efficacy (OR=0.51, 95% CI: 0.27- 0.96). High levels of burnout were reported at this medical school. Although the burnout level is not significantly associated with the frequency of involvement in extracurricular activities, leading and organizing extracurricular activities might result in lower levels of burnout. Therefore, improving the students' leadership skills should be considered in curriculum planning, and greater emphasis should be placed on the quality of involvement in extracurricular activities rather than the quantity.

  19. Extracurricular Activities and Bullying Perpetration: Results From a Nationally Representative Sample.

    Science.gov (United States)

    Riese, Alison; Gjelsvik, Annie; Ranney, Megan L

    2015-08-01

    Bullying is a widespread problem for school-aged children and adolescents. Interventions to reduce bullying are not well disseminated. Extracurricular involvement is, however, common. This study aims to examine the relationship between parent-reported participation in extracurricular activities and bullying perpetration. Using the 2011 National Survey of Children's Health, 62,215 interviews with parents of children 6 to 17 were analyzed. Extracurricular categories of sports only, sports + nonsport, nonsport only, and no activities were based on parental response to questions regarding sport teams/lessons, clubs, and organizations. Bullying was derived from report of the child "bullying or being cruel/mean to others." Weighted bivariate analyses, logistic regression, and sex/race/ethnicity/age stratified analyses were conducted. Eighty percent of children participated in extracurricular activities: 8% sports, 48% sports + nonsports, and 24% nonsports. Bullying perpetration was reported in 15% of the sample. Compared with those not participating in extracurricular activities, the odds of bullying were significantly lower for children who participated in sports + nonsports (Adjusted odds ratio (AOR) 0.67, 95% confidence interval [CI] 0.57-0.79) and nonsport only (AOR 0.82, 95% CI 0.70-0.97). Stratified analyses showed attenuated effects of extracurricular activities for boys and for Hispanics. Children who participate in a variety of extracurricular activities exhibit the least frequent bullying perpetration. © 2015, American School Health Association.

  20. Extracurricular Involvement Among Affluent Youth: A Scapegoat for “Ubiquitous Achievement Pressures”?

    OpenAIRE

    Luthar, Suniya S.; Shoum, Karen A.; Brown, Pamela J.

    2006-01-01

    It has been suggested that overscheduling of upper-class youth might underlie the high distress and substance use documented among them. This assumption was tested by considering suburban 8th graders’ involvement in different activities along with their perceptions of parental attitudes toward achievement. Results indicated negligible evidence for deleterious effects of high extracurricular involvement per se. Far more strongly implicated was perceived parent criticism for both girls and boys...

  1. TEACHERS, STUDENTS AND EXTRACURRICULAR ACTIVITIES IN PRIMARY EDUCATION

    Directory of Open Access Journals (Sweden)

    Ankica Antovska

    2016-06-01

    Full Text Available In the scientific public, since long ago, there has been an interest in studying leisure time. Leisure time is a central issue of modern civilization, integral part of the structure of modern society and a significant factor for a person’s development. The goal of this research is to study the modern concept of and the benefits from the introduction of leisure (extracurricular activities in the primary education process. Certain investigations show that teachers, through extracurricular activities, can have a significant role in building and improving the talents of students. From the answers received in the enquiry it can be seen that both teachers and students have different attitudes and interests regarding extracurricular activities. The school has still the best position and the hope for developing students’ skills for the wide world of extracurricular activities.

  2. Physical extracurricular activities in educational child-robot interaction

    NARCIS (Netherlands)

    Davison, Daniel Patrick; Schindler, Louisa; Reidsma, Dennis; Dautenhahn, K.; Baxter, P.; Weiss, A.; Salem, A.

    In an exploratory study on educational child-robot interaction we investigate the effect of alternating a learning activity with an additional shared activity. Our aim is to enhance and enrich the relationship between child and robot by introducing ``physical extracurricular activities''. This

  3. Physical extracurricular activities in educational child-robot interaction

    NARCIS (Netherlands)

    Davison, Daniel Patrick; Schindler, Louisa; Reidsma, Dennis; Dautenhahn, K.; Baxter, P.; Weiss, A.; Salem, A.

    2016-01-01

    In an exploratory study on educational child-robot interaction we investigate the effect of alternating a learning activity with an additional shared activity. Our aim is to enhance and enrich the relationship between child and robot by introducing ``physical extracurricular activities''. This enric

  4. The Impact of High School Extracurricular Involvement on the Postsecondary Outcomes of Deaf and Hard-of-Hearing Youth

    Science.gov (United States)

    Schoffstall, Sarah; Cawthon, Stephanie; Dickson, Duncan; Bond, Mark; Ocuto, Oscar; Ge, Jinjin

    2016-01-01

    Involvement in extracurricular activities provides youth with opportunities to develop important personal skills, abilities, and preferences, and to build meaningful social support networks. Historically, students who are deaf or hard of hearing (SDHH) have had limited access to opportunities for both academic and occupational development,…

  5. THE INFLUENCE OF EXTRACURRICULAR ACTIVITIES ON THE ATTRACTIVENESS OF A UNIVERSITY

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    Oana DUMITRASCU

    2014-12-01

    Full Text Available The main goal of this study is to analyse the influence of the extracurricular offer on the attractiveness of the study location, analysing four universities from Germany. This study aims to determine the involvement of students in extracurricular activities, their awareness, and to formulate recommendations for the University of Applied Sciences Worms. The research focuses on the sports activities offer. The study has been accomplished using the bibliographic study, the methodology of qualitative and quantitative research, using various secondary and primary sources. Using the survey method, data from 699 students from Germany, registered in the university year 2013/2014 were gathered. The collected data were analysed through univariate and bivariate analysis. As a result of the study, specific gaps from each region are identified regarding the extracurricular offer of the analysed universities and recommendations for the University of Applied Sciences Worms are formulated.

  6. Extra-Curricular Activities and Academic Performance in Secondary Students

    Science.gov (United States)

    Moriana, Juan Antonio; Alos, Francisco; Alcala, Rocio; Pino, Maria-Jose; Herruzo, Javier; Ruiz, Rosario

    2006-01-01

    Introduction: In this paper we study the possible influence of extra-curricular activities (study-related and/or sports) on academic performance of first- and second-year pupils in "Educacion Secundaria Obligatoria (ESO)" [N.T. seventh- and eighth-graders]. Method: We randomly selected 12 schools in the city (9 public and 3 private), and…

  7. Extra-Curricular Activities and Academic Performance in Secondary Students

    Science.gov (United States)

    Moriana, Juan Antonio; Alos, Francisco; Alcala, Rocio; Pino, Maria-Jose; Herruzo, Javier; Ruiz, Rosario

    2006-01-01

    Introduction: In this paper we study the possible influence of extra-curricular activities (study-related and/or sports) on academic performance of first- and second-year pupils in "Educacion Secundaria Obligatoria (ESO)" [N.T. seventh- and eighth-graders]. Method: We randomly selected 12 schools in the city (9 public and 3 private), and…

  8. School Physical Education, Extracurricular Sports, and Lifelong Active Living

    Science.gov (United States)

    Bocarro, Jason; Kanters, Michael A.; Casper, Jonathan; Forrester, Scott

    2008-01-01

    The purpose of this article is to examine the role of school-based extracurricular initiatives in facilitating immediate and long-term positive impact on physical activity, healthy behavior, and obesity in children. A critique of the role of various sports-related initiatives that have been developed to address the obesity epidemic currently…

  9. Extra-Curricular Activities and Academic Performance in Secondary Students

    Science.gov (United States)

    Moriana, Juan Antonio; Alos, Francisco; Alcala, Rocio; Pino, Maria-Jose; Herruzo, Javier; Ruiz, Rosario

    2006-01-01

    Introduction: In this paper we study the possible influence of extra-curricular activities (study-related and/or sports) on academic performance of first- and second-year pupils in "Educacion Secundaria Obligatoria (ESO)" [N.T. seventh- and eighth-graders]. Method: We randomly selected 12 schools in the city (9 public and 3 private), and randomly…

  10. Extracurricular Physical Activity Programs in California Private Secondary Schools.

    Science.gov (United States)

    Kahan, David; McKenzie, Thomas L

    2017-09-20

    Interscholastic, intramural, and club physical activity (PA) programs can be important contributors to student PA accrual at schools. Few studies have assessed factors related to the provision of these extracurricular PA programs, especially in private schools. We used a 16-item questionnaire to assess the associations and influences of selected factors relative to extracurricular PA program policies and practices in 450 private California secondary schools. Associations were evaluated using contingency table analyses (i.e., chi-squared, effect size, and post-hoc analyses). Six factors were associated with schools providing extracurricular PA programs: school location, level, enrollment, and religious classification and whether the physical education (PE) program met state PE time standards and was taught by PE specialists. Both static factors (e.g., school location, level, enrollment, and religious affiliation) and modifiable factors (e.g., meeting PE standards and employing specialists) affect the provision of extracurricular PA programs. As education is state-mandated, additional study is recommended to assess the generalizability of these findings to other states and to public schools.

  11. Physical extracurricular activities in educational child-robot interaction

    OpenAIRE

    Davison, Daniel; Schindler, Louisa; Reidsma, Dennis

    2016-01-01

    In an exploratory study on educational child-robot interaction we investigate the effect of alternating a learning activity with an additional shared activity. Our aim is to enhance and enrich the relationship between child and robot by introducing "physical extracurricular activities". This enriched relationship might ultimately influence the way the child and robot interact with the learning material. We use qualitative measurement techniques to evaluate the effect of the additional activit...

  12. [Study of parental participation in curricular and extracurricular activities].

    Science.gov (United States)

    Renaud, L; Mannoni, C

    1997-01-01

    This preliminary study of parental participation in curricular and extra-curricular activities was conducted in the general framework of the healthy heart project in St. Louis du Parc, an underprivileged and multiethnic neighbourhood in Montreal. A general understanding of this type of participation is essential in order to adapt the role parents play in health programs developed in conjunction with the school. A conceptual framework was developed to help understand how and why parents of primary school children participate in school activities. Qualitative analysis of 15 semi-directed interviews conducted with various schools and other organizations revealed those obstacles to parental involvement, and the factors that encourage parents to participate. It was clear that there were wide social and cultural gaps between parents and schools. An important factor is how people perceive those barriers. Teachers stressed organizational and language communications difficulties. Others pointed to the lack of knowledge of the community, to poor communications skills and the parents' lack of those skills that would allow them to participate actively. A more open-minded attitude by school authorities and a more proactive and positive attitude of teachers toward parents are essential requirements, but not the only ones. Further interviews with parents should help provide more details and information.

  13. How to Develop the DARP-Oriented English Extracurricular Activities

    Institute of Scientific and Technical Information of China (English)

    韩美娟

    2007-01-01

    @@ Just like learning any other foreign languages, English learning needs a more authentic and effective language environment, in which learners learn for using instead of learning for learning. But the present situation in China is not very optimistic, especially in the field of middle school English teaching, English extracurricular activities, as effective methods of providing a real English environment, has not yet been taken seriously by many English teachers. Students still learn English in a deaf-and-dumb environment.

  14. Better Education at Ishik University Preparatory School with Extracurricular Activities

    Directory of Open Access Journals (Sweden)

    Yunus Yildiz

    2015-08-01

    Full Text Available It cannot be said that education today in institutions is better than the previous century. Because in the past, students’ mind was not as full of time-consuming things like spending enormous time in front of a computer or a television as today. Subsequently, teachers used to concentrate their job well and students used to focus on the study better because there was nothing serious except studying. On the other hand, it can be said that the youth today are not eager to learn as yesterday. Because of the fact that they are economically free and supported, they do not worry about failing in the exams supposing that they can possess the questions somehow easily and pass the exams. As a result, they think that whatever they want, they can simply gain without pain. Doing homework or extra studies are a torture from students' perspective. As lecturers we are we have to find much more tactics to motivate students in this education era. Hence, extracurricular activities might play a great role for motivating them to study and on their study achievement if they are used in institutions effectively. In my research article educators’ observations and extracurricular activities’ positive impact on weak language learners will be mentioned. Keywords: Extracurricular, impact, observation, education, achievement

  15. REMOTE ORGANIZATION OF EXTRACURRICULAR ACTIVITIES OF CHILDREN DISABILITIES

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    S. N. Begidova

    2016-01-01

    Full Text Available The article deals with the problem of creating a favorable conditions for socialization, development of creative abilities and capacities of children with disabilities who are trained on the individual program through the use of modern educational technologies. The notion of remote organization of extracurricular activities for disabled children. Distance education is seen as a crucial technology of special tasks of correctional character, providing creative development of personality, the formation of socially significant qualities, social adaptation and integration in society of disabled children who can not regularly attend school. The article describes the organization developed by the authors on remote  technology extracurricular  activities  for  disabled children, the purpose of which was the inclusion of this category of children in the active social life of the school community, improving personal self-esteem, development  of  creative  abilities.  The  experimental work   was attended by children with disabilities who are on home-based learning. There were the pupils of 6,7 and 9 classes. All students disabled, have disorders of the musculoskeletal system. The results of the implementation of this technology, which allows stating the positive changes in children  with  disabilities,  so they increased communicative level, determination and self-esteem. Parents noted improvement in the mood of the children, their activity, self-confidence, as well as the marked reduction in feelings of loneliness, uselessness. Thus, the experimental work has confirmed the effectiveness of the technology for the remote organization of extracurricular activities for disabled children.

  16. STUDENT CLUBS AS A FORM OF EXTRACURRICULAR ACTIVITIES IN HIGH SCHOOL

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    Irina Vasilievna Tolstoukhova

    2016-02-01

    Full Text Available The students as a specific stratum of young people actively involved in various associations, movements. Today, more than ever need the support of positive student associations from universities. This paper considers the extracurricular activities of students, and in particular the creation and development of a student club that promotes self-learning, self-education and self-development of students.Purpose: to develop the author a mechanism for the development of activities of the student club «Studio».Methodology: a theoretical analysis of the pedagogical literature on the problems of students ‘ extracurricular activities.Results: the experience of the Tyumen oil and gas University on the organization and management of extracurricular educational work with students. The basis of this system became the tradition and the experience of the music club «Studio». Lists the sessions conducted with students for career mobility, career advancement. In conclusion traces the development of motivation of students, who are members of a student club. The paper presents information from the personal experience of the authors.

  17. The Relationship between Life Satisfaction, Social Interest, and Frequency of Extracurricular Activities among Adolescent Students.

    Science.gov (United States)

    Gilman, Rich

    2001-01-01

    Studied the relationship between life satisfaction, social interest, and participation in extracurricular activities in 321 high school students. Higher social interest was significantly related to higher levels of overall satisfaction, and adolescents who participated in more structured extracurricular activities reported higher school…

  18. A Literature Review of the Impact of Extracurricular Activities Participation on Students' Academic Performance

    Science.gov (United States)

    Seow, Poh-Sun; Pan, Gary

    2014-01-01

    Extracurricular activities (ECA) have become an important component of students' school life and many schools have invested significant resources on extracurricular activities. The authors suggest three major theoretical frameworks (zero-sum, developmental, and threshold) to explain the impact of ECA participation on students' academic…

  19. Australian Adolescents' Extracurricular Activity Participation and Positive Development: Is the Relationship Mediated by Peer Attributes?

    Science.gov (United States)

    Blomfield, Corey; Barber, Bonnie

    2010-01-01

    Adolescent participation in extracurricular activities is associated with numerous positive outcomes, yet the mechanisms underlying this relationship are largely unknown. This study had two goals: to investigate the association between participation in extracurricular activities and indicators of positive and negative development for Australian…

  20. Exploring the Roles of Extracurricular Activity Quantity and Quality in the Educational Resilience of Vulnerable Adolescents: Variable- and Pattern-Centered Approaches.

    Science.gov (United States)

    Peck, Stephen C; Roeser, Robert W; Zarrett, Nicole; Eccles, Jacquelynne S

    2008-01-01

    This longitudinal study examines how extracurricular activity involvement contributes to "educational resilience"-the unexpected educational attainments of adolescents who are otherwise vulnerable to curtailed school success due to personal- and social-level risks. Educationally vulnerable youth characterized by significant risks and an absence of assets were identified during early adolescence (approximately age 14) using measures of academic motivation, achievement, and mental health as well as family, school, and peer contexts. Using a mixture of variable- and pattern-centered analytic techniques, we investigate how both the total amount time that vulnerable youth spent in positive extracurricular activities and the specific pattern of their extracurricular activity involvement during late adolescence (approximately age 17) predict their subsequent enrollment in college during early adulthood (up through approximately age 21). Educational resilience was predicted uniquely by some, but not all, activity patterns. These results suggest that positive extracurricular activity settings afford vulnerable youth developmentally appropriate experiences that promote educational persistence and healthy development.

  1. Exploring intercultural competence through an intercultural extracurricular activity in Taiwan

    Directory of Open Access Journals (Sweden)

    Liu Kai Li

    2016-01-01

    Full Text Available This study used interviews to explore how 10 Taiwanese college students' intercultural competence was developed after participating in an intercultural extracurricular activity designed with a purpose to facilitate foreign students’ study in Taiwan and to promote meaningful home-foreign student contacts. In-depth information and narratives from the interviews were collected as evidence of intercultural competence development. The results showed all the interviewees appreciated the experience and felt it to have been of value to them in terms of building international friendships, heightening their intercultural awareness and the benefits of intercultural competence, and having more appreciation and better understanding of Taiwanese culture. Students also realized that a lack of understanding of Taiwanese culture could exacerbate communication difficulties. Students thus became aware of the importance of describing Taiwanese cultural practices in English and realized that more language practice was necessary for smoother communication. Suggestions for both extracurricular activities and language programs improvement in the future were also provided in the conclusion.

  2. Differences in Academic Achievement of Students Involved in Extracurricular Activities in Seventh-Day Adventist Schools in the United States and Bermuda

    Science.gov (United States)

    Sandiford, Anderson P.

    2013-01-01

    This study examined differences in the academic performance of students in grades 6, 7, 8, 9, and 11 in Seventh-day Adventist schools in the United States and Bermuda based on their level of involvement in school music organizations--band or choir--and school sports--varsity or intramural--as measured by standardized achievement and ability tests.…

  3. USE OF INFORMATION TECHNOLOGIES IN EDUCATIONAL EXTRACURRICULAR ACTIVITIES IN HIGHER TOURIST INSTITUTIONS

    Directory of Open Access Journals (Sweden)

    Yuliia O. Matviyiv-Lozynska

    2013-04-01

    Full Text Available The article deals with extracurricular educational activities in higher educational establishment of tourism profile with information technologies usage. It is known that extracurricular activities of higher educational establishment has an impact on the professional activities of future specialists in the tourism industry, as is in the process of extracurricular activities students can put into practice the obtained knowledge and skills. The task of teachers is to build a learning process, in particular activities outside classrooms as its component, so that the students were interested in it. In the modern world of tourism prosperity it is very difficult to do without the usage of multimedia technologies (internet, media, etc.

  4. Extracurricular Sport Activities and their Importance in Children Socialization and Integration Process

    OpenAIRE

    Pomohaci Marcel; Sopa Ioan Sabin

    2017-01-01

    The importance of sports activities nowadays is showing their effects on the future development of children, extracurricular activities come in the help of the physical education teacher with benefits on children's development. Our study focuses on revealing the importance of extracurricular sports activities in the process of children's socialization and integration. The research sample was formed from two different groups, the experimental group was formed by 25 students (age 10 ± 2.1 years...

  5. Extracurricular activities associated with stress and burnout in preclinical medical students.

    Science.gov (United States)

    Fares, Jawad; Saadeddin, Zein; Al Tabosh, Hayat; Aridi, Hussam; El Mouhayyar, Christopher; Koleilat, Mohamad Karim; Chaaya, Monique; El Asmar, Khalil

    2016-09-01

    This study aims to assess the prevalence of stress and burnout among preclinical medical students in a private university in Beirut, Lebanon, and evaluate the association between extracurricular involvement and stress and burnout relief in preclinical medical students. A cross-sectional survey was conducted on a random sample of 165 preclinical medical students. Distress level was measured using the 12-item General Health Questionnaire (GHQ-12) while that of burnout was measured through the Maslach Burnout Inventory-Student Survey (MBI-SS). The MBI-SS assesses three interrelated dimensions: emotional exhaustion, cynicism, and academic efficacy. Extracurricular activities were divided into four categories: physical exercise, music, reading, and social activities. All selected participants responded. A substantial proportion of preclinical medical students suffered from stress (62%) and burnout (75%). Bivariate and multivariate regression analyses revealed that being a female or a 1st year medical student correlated with higher stress and burnout. Music-related activities were correlated with lower burnout. Social activities or living with parents were associated with lower academic efficacy. The high stress and burnout levels call for action. Addressing the studying conditions and attending to the psychological wellbeing of preclinical medical students are recommendations made in the study.

  6. Bonding, Achievement, and Activities: School Bonding, Academic Achievement, and Participation in Extracurricular Activities

    Science.gov (United States)

    Howard, Anissa K.; Ziomek-Daigle, Jolie

    2009-01-01

    Utilizing a single-group interrupted time series design (Creswell, 2003), this pilot study examined the relationship between academic achievement, school bonding, and the extracurricular activity participation of "uninvolved" students (n=11) who participated in a voluntary support group at a suburban high school in the southeast. Results…

  7. Effects of physical education, extracurricular sports activities, and leisure satisfaction on adolescent aggressive behavior: A latent growth modeling approach

    National Research Council Canada - National Science Library

    Sanghyun Park; Weisheng Chiu; Doyeon Won

    2017-01-01

    The present study aimed to investigate the longitudinal influence of physical education classes, extracurricular sports activities, and leisure satisfaction on aggressive behavior among South Korean adolescents...

  8. About Extra-Curricular Activities of The University Graduate – Student’s Viewpoint

    Directory of Open Access Journals (Sweden)

    Ivanova Veronica

    2017-01-01

    Full Text Available Extra-curricular activity of modern youth, including university students, is considered to reduce. Students are thought to demonstrate consumer’s attitude, unwillingness to participate in any social activity, and as a result, the level of soft skills acquired by university students is decreasing. The study of social activity of 100 Bachelor graduates from TPU, including the program “Electrical engineering”, indicated the role of the university in creation of conditions for competence development during training of students according to individual educational trajectory and development of their social (extra-curricular activity.

  9. Motivation towards extracurricular activities and motivation at school: A test of the generalization effect hypothesis.

    Science.gov (United States)

    Denault, Anne-Sophie; Guay, Frédéric

    2017-01-01

    Participation in extracurricular activities is a promising avenue for enhancing students' school motivation. Using self-determination theory (Deci & Ryan, 2000), the goal of this study was to test a serial multiple mediator model. In this model, students' perceptions of autonomy support from their extracurricular activity leader predicted their activity-based intrinsic and identified regulations. In turn, these regulations predicted their school-based intrinsic and identified regulations during the same school year. Finally, these regulations predicted their school-based intrinsic and identified regulations one year later. A total of 276 youths (54% girls) from disadvantaged neighborhoods were surveyed over two waves of data collection. The proposed mediation model was supported for both types of regulation. These results highlight the generalization effects of motivation from the extracurricular activity context to the school context. Copyright © 2016 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved.

  10. Participation in Extracurricular Activities and Emotional and Behavioral Adjustment in Middle Childhood in Spanish Boys and Girls

    Science.gov (United States)

    Molinuevo, Beatriz; Bonillo, Albert; Pardo, Yolanda; Doval, Eduardo; Torrubia, Rafael

    2010-01-01

    This study examined the concurrent relationship between participation in extracurricular activities and externalizing and internalizing problems and social school behavior in a Spanish community sample, separately for boys (n=439) and girls (n=428), enrolled in 2nd, 4th, or 6th grades. Weekly participation in extracurricular activities was related…

  11. THE ROLE OF EXTRACURRICULAR ACTIVITIES AND THEIR IMPACT ON LEARNING PROCESS

    Directory of Open Access Journals (Sweden)

    Claudia Cordea

    2014-07-01

    In terms of entrepreneurial learning student's engagement in extracurricular activities provides enhanced opportunities for 'learning by doing' through action and experience. The data show that an increased action leads to reflective practice and social learning is important for students. The article highlights the capacity of entrepreneurship education to stimulate entrepreneurial learning; illustrating the value of extracurricular activities and explaining how develop student's enterprising behaviours. Furthermore, it is very important to examine the need to develop a more enterprising approach to learning by adopting an experiential approach.

  12. Participation in Home, Extracurricular, and Community Activities among Children and Young People with Cerebral Palsy

    Science.gov (United States)

    Orlin, Margo N.; Palisano, Robert J.; Chiarello, Lisa A.; Kang, Lin-Ju; Polansky, Marcia; Almasri, Nihad; Maggs, Jill

    2010-01-01

    Aim: Participation in home, extracurricular, and community activities is a desired outcome of rehabilitation services for children and young people with cerebral palsy (CP). The purpose of this study was to investigate the effect of age and gross motor function on participation among children and young people with CP. Method: Five hundred…

  13. Socioemotional Behavior and School Achievement in Relation to Extracurricular Activity Participation in Middle Childhood

    Science.gov (United States)

    Metsapelto, Riitta-Leena; Pulkkinen, Lea

    2012-01-01

    This 3-year longitudinal study investigated the associations of student (aged 9 to 10 years at the beginning of the study; n = 281; 51% girls) participation in extracurricular activities with teacher-rated socioemotional behavior and school achievement. MANOVA results showed that, after controlling for the grade level and the initial level of the…

  14. The formation of logic thinking of junior schoolchildren during some extracurricular activities

    Directory of Open Access Journals (Sweden)

    Efimov Vladimir Fedorovich

    2016-04-01

    Full Text Available The article discusses the topical issue of formation of junior schoolchildren's mathematical abilities, discloses the modern scientific concept of General and specific mathematical abilities. The structure of mathematical abilities, analyzed the specificity of extra-curricular activities, which under favorable conditions accompanies the intellectual development of junior schoolchildren.

  15. Extracurricular Activity Participation of Hispanic Students: Implications for Social Capital Outcomes

    Science.gov (United States)

    Villarreal, Victor; Gonzalez, Jorge E.

    2016-01-01

    The authors investigated whether participation in school-based extracurricular activities would predict social and behavioral outcomes (school membership, peer prosocial orientation, and prosocial behavior) associated with school social capital in a group of Hispanic middle school students from the United States of America. Results of hierarchical…

  16. Possibilities of Extracurricular Activities in the Student's Spiritual and Moral Formation

    Science.gov (United States)

    Sadykova, Saule A.; Yergazina, Aliya A.; Yeshpanov, Vladimir S.; Korvyakov, Valeriy A.; Aitzhanova, Akmaral B.

    2016-01-01

    With the purpose of substantiating pedagogical terms of the student's spiritually-moral becoming it is necessary to expose possibilities of extracurricular activities during this process. Its intrinsic characteristics are described as a factor of the student's spiritually-moral becoming. The main idea of the article is to reveal possibilities of…

  17. Timetabling and Extracurricular Activities: A Study of Teachers' Attitudes towards Preparation Time

    Science.gov (United States)

    Whiteley, Robert F.; Richard, George

    2012-01-01

    Many models of timetabling exist in secondary schools in Western educational jurisdictions. This study examines whether or not teachers teaching a full course load without preparation time during a semester are willing to volunteer to participate in extracurricular activities. This research was conducted in a rural school district in British…

  18. Outsourcing Extra-Curricular Activities: A Management Strategy in a Time of Neoliberal Influence

    Science.gov (United States)

    Ng, Shun Wing; Chan, Tsan Ming Kenneth; Yuen, Wai Kwan Gail

    2017-01-01

    Purpose: The purpose of this paper is to report on an exploratory study designed to illuminate the complexity of outsourcing extra-curricular activities (ECAs) in primary schools in a time of neoliberal influence and to examine the views of teaching professionals on the reasons, issues and considerations of outsourcing ECAs such as the dynamic…

  19. Physical Activity Patterns in Portuguese Adolescents: The Contribution of Extracurricular Sports

    Science.gov (United States)

    Silva, Pedro; Sousa, Michael; Aires, Luisa; Seabra, Andre; Ribeiro, Jose; Welk, Gregory; Mota, Jorge

    2010-01-01

    In Portugal, two sports systems exist, one through schools and the other in community clubs. The purpose was to determine the impact of extra-curricular sports (EC sports) on boys' and girls' moderate to vigorous physical activity (MVPA). 208 adolescents (79 boys and 129 girls), between 12 and 18 years old, wore an accelerometer over seven days.…

  20. Inequity outside the Classroom: Growing Class Differences in Participation in Extracurricular Activities

    Science.gov (United States)

    Snellman, Kaisa; Silva, Jennifer M.; Putnam, Robert D.

    2015-01-01

    In this article, the authors report on research that shows that extracurricular activities help cultivate the skills, connections, and knowledge that prepare children for lifelong success. They add, however, that low-income students are increasingly being excluded from participating. Struggling with budget cuts and deficits, many school districts…

  1. Integration of Active Video Games in Extracurricular Activity at Schools

    Science.gov (United States)

    Lee, Jung Eun; Huang, Charles; Pope, Zachary; Gao, Zan

    2015-01-01

    Active video games require players to be physically active. Dance Dance Revolution (DDR) is an interactive dancing game that requires fast-foot movement coordinated with energetic music and visuals. The Wii and Xbox Kinect games have also become good active video games for the promotion of physical activity participation. These games are much more…

  2. Extracurricular Sport Activities and their Importance in Children Socialization and Integration Process

    Directory of Open Access Journals (Sweden)

    Pomohaci Marcel

    2017-06-01

    Full Text Available The importance of sports activities nowadays is showing their effects on the future development of children, extracurricular activities come in the help of the physical education teacher with benefits on children's development. Our study focuses on revealing the importance of extracurricular sports activities in the process of children's socialization and integration. The research sample was formed from two different groups, the experimental group was formed by 25 students (age 10 ± 2.1 years, 13 boys, and 12 girls, that practiced 2 hours a week physical education and other 2 hours a week extracurricular activities like basketball and volleyball; and the sample group formed by 24 students (age 10 ± 1.8 years, 12 boys and 12 girls, that practiced 2 hours a week classic physical education. The results showed us that extracurricular activities have a good impact on children's socialization and integration, the experimental group improved their coefficient of group cohesion from 0.04 at the initial testing to 0.06 at the final test, and the index of cohesion from 0.02 at the initial test to 0.05 at the final testing. Significant differences were found within the experimental group both in the initial and in the final tests (p < 0.05, also significant differences were discovered between the experimental and the sample group in the final measurement (p < 0.05. Conclusions of this research presented that the experimental group had good improvements of cohesion and social integration of children, showing that extracurricular sport activities have a positive impact on improving socialization, developing cohesion and integrate the marginalized children in the social group.

  3. Associations Between Extracurricular Activity and Self-Regulation: A Longitudinal Study From 5 to 10 Years of Age.

    Science.gov (United States)

    Piché, Geneviève; Fitzpatrick, Caroline; Pagani, Linda S

    2015-01-01

    Health promotion in youth is likely to benefit from enhancing academic achievement and physical activity. The present study examines how kindergarten childhood self-regulation skills and behaviors predict involvement in both structured and unstructured physical and nonphysical extracurricular activities in the fourth grade. As a second objective this study also investigated how kindergarten childhood participation in extracurricular activities predicts classroom engagement, reflective of self-regulation, by the fourth grade. Secondary analyses were conducted using prospective-longitudinal data. The Quebec Longitudinal Study of Child Development, Quebec, Canada. Participants were randomly selected at birth from a stratified sample of 2694 born in Québec, Canada, between 1997 and 1998. Participants were included if they had complete data on teacher ratings of child self-regulation as measured by classroom engagement and parent ratings of sports participation (n = 935). Teachers reported self-regulation skills in children through a measure of classroom engagement. Parents provided reports of child participation extracurricular activities. Ordinary least-squares regressions were conducted. A higher-frequency kindergarten involvement with structured physical activities was associated with fourth-grade classroom engagement (β = .061, 95% confidence interval [CI]: .017, .104). Better kindergarten classroom engagement predicted more frequent participation in fourth-grade structured physical activities (β = .799, 95% CI: .405, 1.192) and team sports (β = .408, 95% CI: .207, .608). Results suggest mutual relations between physical activity and self-regulation from kindergarten to grade four. This suggests strong learning skills indicative of self-regulation and opportunities to participate in supervised physical activities or sports teams may help children develop healthy dispositions and behaviors in emerging adolescence.

  4. Extra-curricular physical activity and socioeconomic status in Italian adolescents.

    Science.gov (United States)

    La Torre, Giuseppe; Masala, Daniele; De Vito, Elisabetta; Langiano, Elisa; Capelli, Giovanni; Ricciardi, Walter

    2006-01-31

    The relationship between physical activity and health status has been thoroughly investigated in several studies, while the relation between physical activity and socio-economic status (SES) is less investigated. The aim of this study was to measure the extra-curricular physical activity of adolescents related to the socio-economic status (SES) of their families. The survey was carried out by submitting an anonymous questionnaire to junior high school students in the following Regions: Lazio, Abruzzo, Molise, Campania, Puglia, during the school year 2002-2003. Extra-curriculum physical activity was evaluated considering whether or not present and hours of activity weekly conducted. 2411 students agreed to participate in the study. Participants were 1121 males (46.5%) and 1290 females (53.5%), aged between 11 and 17 years (median age: 12 years). 71.1% of the students reported to practice extra-curricular physical activity. Parents' educational levels and work activities play an important role in predicting students' physical activity, with the more remunerative activities and higher educational levels being more predictive. The results confirm the relationship between adolescents' physical activity and their families' SES. In particular, a positive relationship between participation in extra-curricular physical activity and their families high SES was found. These data will be useful for school administrators and for politicians in order to reduce the gap between adolescents from the least and most disadvantaged families.

  5. Extra-curricular physical activity and socioeconomic status in Italian adolescents

    Directory of Open Access Journals (Sweden)

    Langiano Elisa

    2006-01-01

    Full Text Available Abstract Background The relationship between physical activity and health status has been thoroughly investigated in several studies, while the relation between physical activity and socio-economic status (SES is less investigated. The aim of this study was to measure the extra-curricular physical activity of adolescents related to the socio-economic status (SES of their families. Methods The survey was carried out by submitting an anonymous questionnaire to junior high school students in the following Regions: Lazio, Abruzzo, Molise, Campania, Puglia, during the school year 2002–2003. Extra-curriculum physical activity was evaluated considering whether or not present and hours of activity weekly conducted. 2411 students agreed to participate in the study. Results Participants were 1121 males (46.5% and 1290 females (53.5%, aged between 11 and 17 years (median age: 12 years. 71.1% of the students reported to practice extra-curricular physical activity. Parents' educational levels and work activities play an important role in predicting students' physical activity, with the more remunerative activities and higher educational levels being more predictive. Conclusion The results confirm the relationship between adolescents' physical activity and their families' SES. In particular, a positive relationship between participation in extra-curricular physical activity and their families high SES was found. These data will be useful for school administrators and for politicians in order to reduce the gap between adolescents from the least and most disadvantaged families.

  6. Brief report: Performing on the stage, the field, or both? Australian adolescent extracurricular activity participation and self-concept.

    Science.gov (United States)

    Blomfield, Corey J; Barber, Bonnie L

    2009-06-01

    The relationship between Australian adolescents' participation in extracurricular activities and their self-concepts was investigated. A total of 1489 adolescents (56% female; mean age 13.8 years) completed measures of social self-concept, academic self-concept, and general self-worth, and reported on their extracurricular activity participation. In general, participation in any type of extracurricular activity was associated with a higher social and academic self-concept, and general self-worth, compared to no participation. Adolescents who participated in both sports and non-sports also reported a more positive social self-concept and general self-worth, compared to those who only participated in one of the activity types. This research provides support for extracurricular activities as a context facilitative of positive self-concept, and demonstrates the importance of a mixed participation profile for an adolescent's self-concept.

  7. Developmental experiences during extracurricular activities and Australian adolescents' self-concept: particularly important for youth from disadvantaged schools.

    Science.gov (United States)

    Blomfield, Corey J; Barber, Bonnie L

    2011-05-01

    Extracurricular activities provide adolescents with a number of positive personal and interpersonal developmental experiences. This study investigated whether developmental experiences that occurred during extracurricular activities were linked to a more positive self-concept for Australian adolescents, and whether this link was particularly salient for youth from disadvantaged schools. Adolescents (N = 1,504, 56% Female) from 26 diverse high schools across Western Australia were surveyed. The findings revealed that adolescents from low socio-economic status schools who participated in extracurricular activities had a more positive general self-worth and social self-concept than adolescents from similar socio-economic schools who did not participate in any extracurricular activities. Furthermore, the positive developmental experiences that occurred during extracurricular activities predicted a more positive general self-worth and social and academic self-concept, and this link was stronger for youth from low SES schools. These findings suggest that the developmental experiences afforded by extracurricular activities may foster positive adolescent development.

  8. Extracurricular Activities Targeted towards Increasing the Number of Engineers Working in the Field of Precision Agriculture

    DEFF Research Database (Denmark)

    Larsen, Leon Bonde; Stark Olsen, Kent; Ahrenkiel, Linda

    in agriculture and agricultural technology. This article presents the results of an interdisciplinary extracurricular activity for first year engineering students carried out in the Fall 2012 at the University of Southern Denmark. The case was based on practical group-work centered around an agricultural mobile......, and some of them have on their own initiative engaged in subsequent educational activities within precision agriculture....... robot. The activity was called Practical experience from day one and its primary objective was to improve the participants’ practical experience and give them a broader view of technology applied in agriculture. Based on evaluation of the activities it is concluded that working with a small...

  9. Education of natural science in the work of the Municipal Center for Extracurricular Activities

    Science.gov (United States)

    Jokin, I.

    2012-04-01

    In the description of my work I presented my own experience in the organizing and carrying out of extracurricular activities with the students, the used modes and methods of work, the obtained results and some good practices in the field of natural sciences. Organizing and carrying out of scientific festivals, participation in joint projects together with scientific organizations. Key words: European dimension, interactive methods, key competences, natural sciences, extracurricular activities. We are witnesses of a fundamental change in the pedagogical culture and practice in our schools to establish the parameters of the quality of training. The good scientific culture is an important part of the students' education. Unfortunately, at the present time the scientific and technological culture is on a low level. One of the contemporary problems and realities of the education in natural science school subjects, as a whole and in particular in the secondary education, is the decreased interest for the training in them and in particular in physics, as well as synchronization of the interrelations: school environment - society. In many countries there is a drop in the orientation of the students towards the science and technology - the problem of Science and Technology (S&T). The training of the young people often creates some problems. The teachers meet with the problem of insufficient motivation of the learners for study and difficulties that they encounter in the process of training. The students find it difficult to apply the mastered knowledge to an applied context. The knowledge is rather academic and rather remote from the context, in which the children live and communicate, which makes it nonfunctional. At present there are not enough extracurricular activities that should meet these necessities of the Bulgarian school. The reasons are various, but they mainly consist in the lack of a material base, an exchange of experience and good practices and motivation

  10. Adolescents' physical activity in physical education, school recess, and extra-curricular sport by motivational profiles.

    Science.gov (United States)

    Mayorga-Vega, Daniel; Viciana, Jesús

    2014-06-01

    The main purpose of this study was to evaluate the differences in adolescents´ objective physical activity levels and perceived effort in physical education, school recess, and extra-curricular organized sport by motivational profiles in physical education. A sample of 102 students 11-16 yr. old completed a self-report questionnaire assessing self-determined motivation toward physical education. Subsequently, students' objective physical activity levels (steps/min., METs, and moderate-to-vigorous physical activity) and perceived effort were evaluated for each situation. Cluster analysis identified a two-cluster structure: "Moderate motivation toward physical education profile" and "High motivation toward physical education profile." Adolescents in the second cluster had higher physical activity and perceived effort values than adolescents in the first cluster, except for METs and moderate-to-vigorous physical activity in extra-curricular sport. These results support the importance of physical education teachers who should promote self-determined motivation toward physical education so that students can reach the recommended physical activity levels.

  11. Another Way to Develop Chinese Students' Creativity: Extracurricular Innovation Activities

    Science.gov (United States)

    Xiao-jiang, Zhao; Xue-ting, Zhao

    2012-01-01

    In China, improving students' creativity is becoming an important goal of modern colleges and universities, especially in the domain of science and technology. The efforts made for this goal can be observed not only in classroom, but also in activities and competitions which were held out-of-school. This paper will firstly give a brief description…

  12. Adolescents' social environment and depression: social networks, extracurricular activity, and family relationship influences.

    Science.gov (United States)

    Mason, Michael J; Schmidt, Christopher; Abraham, Anisha; Walker, Leslie; Tercyak, Kenneth

    2009-12-01

    The present study examined components of adolescents' social environment (social network, extracurricular activities, and family relationships) in association with depression. A total of 332 adolescents presenting for a routine medical check-up were self-assessed for social network risk (i.e., smoking habits of best male and female friends), extracurricular activity level (i.e., participation in organized sports teams, clubs, etc.), family relationship quality (i.e., cohesion and conflict), and symptoms of depression (i.e., minimal, mild, moderate/severe). Results of a forward linear regression modeling indicate that social environment components were associated with a significant proportion of the variance in adolescent depression (Adjusted R (2) = .177, p social network (beta = .107, p relationships (beta = -.368, p social environment plays in adolescent depression, as well as yields new insights into socially-based intervention targets that may ameliorate adolescent depression. These intervention targets may be gender-specific, include positive social network skills training, increase adolescents' engagement in organized activities, and attend to the quality of their family relationships.

  13. Making Meaning of Everyday Practices: Parents' Attitudes toward Children's Extracurricular Activities in the United States and in Italy

    Science.gov (United States)

    Kremer-Sadlik, Tamar; Izquierdo, Carolina; Fatigante, Marilena

    2010-01-01

    This article focuses on children's engagement in extracurricular activities from the perspective of middle-class parents in Rome, Italy, and Los Angeles, California. Analysis of parents' accounts captured in interviews and ethnographic fieldwork reveals that both sets of parents perceive activities as important for children's success. Yet Roman…

  14. Effects of Extracurricular Activities and Their Quality on Primary School-Age Students' Achievement in Mathematics in Switzerland

    Science.gov (United States)

    Schuepbach, Marianne

    2015-01-01

    In this study, we examined the development of mathematics achievement in children attending extracurricular activities intensively in comparison with the development in a control group of children attending only the obligatory hours of school instruction. In addition, we investigated the question of possible effects of intensity of attendance and…

  15. The Effectiveness of Student Extracurricular Activities in Evaluating Violent Behavior among Students in the Preparatory Year at Hail University

    Science.gov (United States)

    Aleid, Alkhamsah Saleh

    2016-01-01

    This study aimed to examine the effectiveness of student extracurricular activities in evaluating violent behavior among students in the preparatory year at Hail University. The researcher used the descriptive analytical method, and used two tools for the purpose of the study, the study sample consisted of 104 (violent) female students from the…

  16. Enhancing Science Education through Extracurricular Activities: A Retrospective Study of "Suzy Science and the Whiz Kids[C]"

    Science.gov (United States)

    Kralina, Linda M.

    2009-01-01

    Extracurricular activities (ECA) are informal settings offering free-choice experiences that are generally voluntary, open-ended, non-sequential, self-directed, hands-on, and evaluation-free. This mixed methods study investigates participation in a high school science ECA by collecting the memories of former student members for their perceptions…

  17. The Impact of Engagement with Extracurricular Activities on the Student Experience and Graduate Outcomes for Widening Participation Populations

    Science.gov (United States)

    Stuart, Mary; Lido, Catherine; Morgan, Jessica; Solomon, Lucy; May, Steve

    2011-01-01

    This research examined extracurricular activity (ECA) effects on students' experiences, outcomes and future job prospects. A survey of diverse undergraduate students, along with alumni and potential employer interviews, revealed differences in students' engagement with ECAs beyond the classroom. Variations between "traditional" and…

  18. School-Based Extracurricular Activities, Personality, Self-Concept, and College Career Development Skills in Chinese Society

    Science.gov (United States)

    Shiah, Yung-Jong; Huang, Ying; Chang, Frances; Chang, Chuan-Feng; Yeh, Lun-Chang

    2013-01-01

    In the present study, we examined in Chinese society the association of school-based extracurricular activities (SBEAs) in both high school and college with students' career development skills in college, as well as with various personality characteristics and self-concept. Each of 281 college students administered the Lai Personality Inventory,…

  19. Ensuring Full Participation in Extra-Curricular Activities for Students Experiencing Homelessness. Best Practices in Homeless Education

    Science.gov (United States)

    National Center for Homeless Education at SERVE, 2010

    2010-01-01

    Extra-curricular school activities, such as sports, music, theater, debate, and clubs, are often a key to engaging children and youth in school. They can provide students with a sense of belonging, stability, pride, and responsibility and strengthen a student's applications for higher education admission and scholarships. Homelessness, however,…

  20. The role of breakfast consumption and participation in extracurricular physical activity in the development of obesity in students from 10 to 12 years old

    National Research Council Canada - National Science Library

    Ioannou Trifon; Mavrovouniotis Fotios; Mavrovouniotis Argirios; Nikitaras Nikitas; Mavrovounioti Chrysi; Fachantidou-Tsiligiroglou Anna; Deligiannis Asterios

    2016-01-01

      The purpose of the present research was to study the role of breakfast consumption and participation in extracurricular physical activity in the development of obesity in Greek students from 10 to 12 years old...

  1. Students’ Extracurricular Activities in Higher Education and Its Effect on Personal Development and Academic Achievement (Case Study In Islamic State University of Suska Riau

    Directory of Open Access Journals (Sweden)

    Amirah Diniaty

    2014-11-01

    Full Text Available This study investigates the students’ extracurricular activities at Islamic State University of Suska Riau concerning the types and forms of their activities, background of the activity, benefits of extracurricular activities, and its effect on students’ self-development and academic achievement. 300 students who enroll at sixth semester in eight Faculties (Tarbiyah and Teacher’s Training, Sharia and Law, Ushuludin, Dakwah and Communication Sciences, Science and Technology, Psychology, Economics and Social Sciences, Agriculture and Animal Husbandry were the sample of this study. The data were then processed statistically using percentages and regression of correlation. It was found that the type of extracurricular followed by the students were leadership (70%, those of whom had the motivation to add insight, knowledge and more critical thinking were (44.67%. Some students followed extra-curricular activity because of their solicitation or to follow the main stream (37%, some students did not get financial benefits from the activity (34.67%. Some other respondents felt that they could follow the extracurricular talents, interests and personal abilities (58.67%, and think about their future after graduating from the college (66%. Things need to be followed up by students and university staffs were the more active students who attend the extracurricular activities were the lower achievement ones. Copyright © 2014 by Al-Ta'lim All right reserved

  2. Students’ Extracurricular Activities in Higher Education and Its Effect on Personal Development and Academic Achievement (Case Study In Islamic State University of Suska Riau

    Directory of Open Access Journals (Sweden)

    Amirah Diniaty

    2014-11-01

    Full Text Available This study investigates the students’ extracurricular activities at Islamic State University of Suska Riau concerning the types and forms of their activities, background of the activity, benefits of extracurricular activities, and its effect on students’ self-development and academic achievement. 300 students who enroll at sixth semester in eight Faculties (Tarbiyah and Teacher’s Training, Sharia and Law, Ushuludin, Dakwah and Communication Sciences, Science and Technology, Psychology, Economics and Social Sciences, Agriculture and Animal Husbandry were the sample of this study. The data were then processed statistically using percentages and regression of correlation. It was found that the type of extracurricular followed by the students were leadership (70%, those of whom had the motivation to add insight, knowledge and more critical thinking were (44.67%. Some students followed extra-curricular activity because of their solicitation or to follow the main stream (37%, some students did not get financial benefits from the activity (34.67%. Some other respondents felt that they could follow the extracurricular talents, interests and personal abilities (58.67%, and think about their future after graduating from the college (66%. Things need to be followed up by students and university staffs were the more active students who attend the extracurricular activities were the lower achievement ones. Copyright © 2014 by Al-Ta'lim All right reserved

  3. Effects of physical education, extracurricular sports activities, and leisure satisfaction on adolescent aggressive behavior: A latent growth modeling approach.

    Science.gov (United States)

    Park, Sanghyun; Chiu, Weisheng; Won, Doyeon

    2017-01-01

    The present study aimed to investigate the longitudinal influence of physical education classes, extracurricular sports activities, and leisure satisfaction on aggressive behavior among South Korean adolescents. Data were drawn from the Korea Youth Panel Survey. We used latent growth curve modeling to explain the growth trajectory of adolescent aggressive behaviors and a multi-group analysis to investigate gender differences in aggressive behavior. The results indicated that adolescents' aggressive behavior significantly changed with age. There were significant gender-based differences in the level of and changes in aggressive behavior over time. Both extracurricular sports activities and leisure satisfaction had significant influences on the changes in adolescents' aggressive behavior with age, whereas physical education classes did not.

  4. It isn't all just fun and games: Collegiate participation in extracurricular activities and risk for generalized and sexual harassment, psychological distress, and alcohol use.

    Science.gov (United States)

    McGinley, Meredith; Rospenda, Kathleen M; Liu, Li; Richman, Judith A

    2016-12-01

    Collegiate extracurricular activities, despite their benefits, may place students at an increased risk for experiencing harassment. This study utilizes multiple waves of data from an online longitudinal survey to examine how participation in college activities (intramural sports, fraternities/sororities, school clubs) relates to experiences of sexual and generalized harassment and outcomes (psychological distress, heavy alcohol use) among undergraduates (N = 1852, 58.6% female, 57.4% White) in the Midwestern United States. Activity participation was related to harassment, but the pattern depended on the activity, harassment type, and sex. Fraternity/sorority involvement was associated with generalized harassment, whereas school club involvement was linked to both generalized and sexual harassment. Female intramural athletes were at an increased risk to experience both harassment types. In addition to direct relations, activity participation was indirectly linked to future psychological distress (depression, anxiety) and heavy alcohol use via harassment. Implications for intervention with this college student population are discussed.

  5. READINESS OF TEACHERS FOR THE ORGANIZATION OF EXTRACURRICULAR ACTIVITIES OF STUDENTS AT HIGHER EDUCATIONAL ESTABLISHMENT OF ECONOMICS

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    L. V. Vishnevetskaja

    2014-09-01

    Full Text Available The urgency of the article, its theoretical and practical importance are caused by the existing contradiction between the state order for the formation of socially active person and lack of scientifically-practical workings out in the field of pedagogical support of the organization of extracurricular socially useful activity of the students. The settlement of the contradiction is possible in the presence of teachers specially prepared for the given process.Purpose. The purpose of the article is the theoretical substantiation and experimental estimation of teachers’ readiness for the organization of extracurricular activities of students in higher education establishments of economics.Methodology, Results. Complex application of theoretical (element and the structural analysis, comparison, generalization and empirical (questioning, testing, the expert estimation, included pedagogical supervision methods has allowed to define the essence of investigated concept as integrative personal formation, to develop criteria and its indicators of its formation and experimentally estimate the level of readiness of teaching staff for the organization of students’ extracurricular activities in higher education establishments of economics.Practical implications. The results of the research can be widely used in the work of curators of academic groups, tutors, teachers-organizers, in the system of training and advanced training of teaching staff.

  6. Extracurricular activities: Investigating the affects of participation-nonparticipation on the Georgia High School Science Graduation Test

    Science.gov (United States)

    Moran, Ray A.

    Student achievement research suggests that participation in extracurricular activities has a positive impact on the academic and developmental outcomes for adolescents. Specifically, several studies reported that adolescents who participate in extra-curricular activities are more likely to experience increases in academic achievement, self-esteem, high school graduation rates, and pro-social behaviors. On the other hand, there is research suggesting that participation in extracurricular activities may distract students from their academic pursuits. The state of Georgia requires all eleventh grade students to participate in the Georgia High School Graduation Test (GHSGT). The GHSGT consists of five separate tests that include (a) English/language arts, (b) math, (c) writing, (d) social studies, and (e) science. Each comprehensive exam is worth 600 points. A high school diploma will be awarded if the student scores at least 500 points on each individual exam. Further, review of student outcomes on the GHSGT revealed that first-time test takers were failing the science portion of the test at a greater percentage than any other subject on the GHSGT. Specifically, the Governor's Office of Student Achievement (GOSA) reported that from 2002 through 2004, a total of 70,451 students or 30.3% of students that were first-time test takers failed the science portion of the GHSGT. As a result, investigating factors that potentially could increase student achievement in science became the impetus for this study. In particular, this study examined the relationships between the levels of student participation in school sponsored extracurricular activities in relation to the level of student achievement in the area of science.

  7. Analysis of Benefits to Young Rail Enthusiasts of Participating in Extracurricular Academic Activities

    Directory of Open Access Journals (Sweden)

    Anna Fraszczyk

    2015-10-01

    Full Text Available The paper examines a number of positive academic outcomes achieved by university students who, as young rail enthusiasts representing over 10 European institutions, took part in extracurricular rail-related academic activities. Analyses presented in the paper are based on the evaluation of the responses to the questionnaire distributed amongst participants of three consecutive editions of an intensive program in rail and logistics which took place in 2012, 2013, and 2014. Also, quotes from follow-up one-to-one interviews with participants are used to support the results presented. The sample for each year varies in terms of numbers, nationalities, academic backgrounds, and male:female ratio. Academic benefits are specifically looked into, which includes teaching and learning activities throughout the three weeks of the program. The analysis of results revealed that young rail enthusiasts who participated in the program benefited from lectures delivered in Week 1, especially those focused on multimodal transport and rail infrastructure in 2013, which received positive mean ratings of 4.45 and 4.53, respectively, on a five-point Likert scale. Moreover, academic benefits were strongly supported by non-technical skills improvements in areas such as English language and communication, both reaching the mean ratings of 4.35 or above in 2014. A number of recommendations for improvements of next editions of similar rail-related programs as well as areas for future research are identified in the paper

  8. The Impact of Extracurricular Activities on Academic Performance for Rural Secondary Students in Indiana

    Science.gov (United States)

    Wilcox, Michael Lee

    2012-01-01

    The purpose of this study was two-fold in nature. First, this study sought to identify whether extracurricular participation for students in a rural, Grades 7-12 building created significant differences when examining academic performance, attendance, gender, lunch status, and student discipline compared to their non-participant peers. Secondly,…

  9. DIAGNOSTICS OF READINESS OF TEACHERS TO THE EXTRACURRICULAR ACTIVITIES OF ELEMENTARY SCHOOL STUDENTS IN ECOLOGICAL AND LOCAL HISTORY

    Directory of Open Access Journals (Sweden)

    Maria Viktorovna Sineva

    2015-05-01

    Full Text Available This paper considers the problem of preparation of the future teacher to the organization of extracurricular activities in environmental (ecological and local history for younger students and dedicated to this aspect in the implementation of the Federal state educational standard of the new generation. It describes the components of professional readiness of the future teacher for this type of activity; analyzes the results of the evaluation of the readiness of the future teachers carried out by the author developed criteria, indicators and diagnostic tools. Materials can be used by teachers of pedagogical universities and teacher training institutes.

  10. Extracurricular participation and academic outcomes: testing the over-scheduling hypothesis.

    Science.gov (United States)

    Fredricks, Jennifer A

    2012-03-01

    There is a growing concern that some youth are overscheduled in extracurricular activities, and that this increasing involvement has negative consequences for youth functioning. This article used data from the Educational Longitudinal Study (ELS: 2002), a nationally representative and ethnically diverse longitudinal sample of American high school students, to evaluate this hypothesis (N = 13,130; 50.4% female). On average, 10th graders participated in between 2 and 3 extracurricular activities, for an average of 5 h per week. Only a small percentage of 10th graders reported participating in extracurricular activities at high levels. Moreover, a large percentage of the sample reported no involvement in school-based extracurricular contexts in the after-school hours. Controlling for some demographic factors, prior achievement, and school size, the breadth (i.e., number of extracurricular activities) and the intensity (i.e., time in extracurricular activities) of participation at 10th grade were positively associated with math achievement test scores, grades, and educational expectations at 12th grade. Breadth and intensity of participation at 10th grade also predicted educational status at 2 years post high school. In addition, the non-linear function was significant. At higher breadth and intensity, the academic adjustment of youth declined. Implications of the findings for the over-scheduling hypothesis are discussed.

  11. The Parents' and Teachers' Supports Role on Students' Involvement in Scouting Program and Entrepreneurial Values--Longitudinal Studies on Students in Jombang, East Java, Indonesia

    Science.gov (United States)

    Prianto, Agus

    2016-01-01

    Extracurricular activities are those that fall outside the realm of the normal curriculum of school. Extracurricular activities exist for all students. And generally, benefits of extracurricular activities shall be as follows: learning time management and prioritizing; getting involved in diverse interests; learning about long term commitments;…

  12. "Journalism, Poetry, Stand-Up Comedy, and Academic Writing: Mapping the Interplay of Curricular and Extracurricular Literate Activities": Re-Visiting a Theoretical Lens Five Years Later

    Science.gov (United States)

    Roozen, Kevin

    2014-01-01

    Published in a 2008 issue of "Journal of Basic Writing" ("JBW"), "Journalism, Poetry, Stand-Up Comedy, and Academic Writing: Mapping the Interplay of Curricular and Extracurricular Literate Activities" was Kevin Roozen's first single-authored publication. Drawn from data collected for the first case study from…

  13. The Participation of Girls and Boys from Ages 10 to 18 in Structured Sports and Extra-Curricular Activities in the Aspect of Social and Economic Conditions

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    Dmitruk Agnieszka

    2015-03-01

    Full Text Available Introduction. The importance of socio-economic factors in differentiating the physical activities of children and teenagers keeps changing. That is why the goal of this research was to identify certain social variables amongst those listed most often which differentiate the level of targeted physical activity of urban children and youths from the Bialskie district. Material and methods. The research was conducted on 1.084 students between the ages of 10 and 18. Three age groups were chosen: 10 to 12, 13 to 15, and 16 to 18. Information about the attendance of respondents in Physical Education classes and structured extra-curricular sport activities, as well as preferred forms of physical activities were collected via a diagnostic survey. The same method was used in the assessment of the socio-economic status of the families of the respondents. The relations between attendance in extra-curricular sports activities and socio-economic factors was assessed by a multifactoral logistic regression model, and the statistical relevance of the differences was calculated by the Wald test. Results. Attendance in structured extra-curricular physical activity depended mostly on income per family member. In the case of type of work performed by parents, the attendance rate in both boys and girls was higher if the parents performed intellectual work. Parents' education and number of children in the family did not have a high influence on the attendance of respondents in structured extra-curricular sport activities. Conclusions. Increasing disproportions in societal prosperity can be an important factor limiting the attendance of the poorest group of children and youths in structured physical activities. The lack of influence on physical activity of such socio-economic variables as parents' education and the number of children in the family can be connected with the change of meaning of those factors in modern society (depreciation of the importance of higher

  14. Extracurricular activities and teens' alcohol use: The role of religious and secular sponsorship.

    Science.gov (United States)

    Adamczyk, Amy

    2012-03-01

    Much research has found that more religious youth are less likely to engage in riskier health-related behaviors. However, very little research has examined the role that religion may play in shaping the health-related behaviors of secular youth. There is reason to think that more and less religious youth may gain some health-related benefits from involvement with religious organizations through activities such as basketball and volunteering. Using two waves of data from the National Study of Youth and Religion, this study finds that involvement in religion-supported secular activities is associated with less alcohol use for all involved teens. The number of friends who belong to a religious youth group, in part, explains the relationship. Conversely, network overlap between parents and teens, the number of friends who drink or use drugs, and having an adult confidant from a religious group are not mechanisms that mediate the relationship.

  15. The Current Situation of Students’ Participatation in Extracurricular Sports Activities of Private Middle School in Henan Province and the Analysis of Investigation

    Science.gov (United States)

    Zhe, Wang

    By using the methods of document literature, questionnaire survey and mathematical statistics, this paper investigates and analyses the cuurent situation of students' participation in extrucurricular sports activities of 36 private middle schools in Henan province which have legal education procedures through the following aspects: the attitude, motivation, times, duration, selection of programs, and influential factors of participating in extracurricular sports activities. Based on the investigation and analysis, this paper points out the existing problems and puts forward suggestions

  16. Gender Variation in Extracurricular Activity Participation and Perceived Life Chances in Trinidad and Tobago Adolescents Variación por Sexo de la Participación en Actividades Extracurriculares y la Percepción de Oportunidades de Vida en Adolescentes de Trinidad y Tobago

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    Zena R Mello

    2008-11-01

    Full Text Available Participation in extracurricular activities has surfaced as an important context for adolescent development and may be a fruitful avenue for fostering future attitudes, a concept shown to predict adolescent behaviors. In this study of youth from Trinidad and Tobago, we examined gender differences in activity participation and perceived life chances in 1,385 adolescents (59% male. Trinidadian adolescents were highly active, on average, participating in three activities. More males were involved in athletic and organized groups, whereas more females participated in artistic and religious activities. Artistic and athletic activity participation predicted positive perceived life chances, even after controlling for academic achievement. Artistic activities were especially beneficial for predicting future attitudes for males compared to females.La participación en actividades extracurriculares es un importante contexto para el desarrollo adolescente y puede ser una provechosa vía para promover actitudes acerca del futuro, un concepto que permite predecir el comportamiento adolescente. En esta investigación se examinaron las diferencias por sexo de la participación en actividades y la percepción de las oportunidades de vida en 1.385 adolescentes de Trinidad y Tobago (59% varones. Los adolescentes eran muy activos, participando en promedio en tres actividades. Los hombres participaban más en deportes y grupos organizados, mientras las mujeres lo hacían en actividades artísticas y religiosas. La participación en actividades artísticas y deportivas predijo una percepción positiva sobre las oportunidades de vida, aun después de controlar los logros académicos. Las actividades artísticas fueron especialmente beneficiosas para predecir las actitudes de los hombres respecto del futuro.

  17. Actual State of the Sports Club Advisors’ Worries and Burdens in Extracurricular Sports Activities at High School : A Preliminary Investigation for the Development of Stressor Scale

    OpenAIRE

    渋倉, 崇行; Shibukura, Takayuki

    2013-01-01

    The purposes of this study are to clarify the actual state of the sports club advisors’ worries and burdens in extracurricular sports activities at high school and to discuss the selection of the survey items that constitute the stressor scale for sports club advisors at high school. In order to collect data, 42 sports club advisors( 31 males and 11 females) in the public high schools participated in this study. They were requested to complete the questionnaire consisting of free descriptive ...

  18. Interações sociais de crianças pré-escolares com Síndrome de Down durante atividades extracurriculares Interacciones sociales de niños en edad preescolar con síndrome de Down durante actividades extracurriculares Social interactions of preschool children with Down syndrome during extracurricular activities

    Directory of Open Access Journals (Sweden)

    Renata Valdívia Lucisano

    2013-02-01

    ños" tuvieran ocurrencia significativa. Los comportamientos más comunes que se encuentran en este estudio demuestran que el entorno escolar se convierte en un facilitador de la interacción del niño con síndrome de Down con otros niños con desarrollo normal, lo que permite el desarrollo de habilidades sociales esperadas.The aim of this research was to identify the process of social interaction of children with Down Syndrome (DS during extracurricular activities in the regular early childhood education in Ribeirão Preto-SP, Brazil. Six children aged 3-6 years participated in this study. There were two recordings of each child in situations of social interaction during extracurricular activities, and analyzed by 15 behaviors, divided into two categories of social skills: interpersonal and self-expression. The results demonstrate that, in the interpersonal skill category, the higher occurrence was the behavior "occurs interaction with other children". In the self-expression skills category, only the behaviors "smiles" and "imitates other children" have significant occurrence. The behaviors more frequently identified in this study permit to understand that the school environment is a facilitator for the interaction of child with DS with the typical developmental children, allowing him/her to develop the expected social skills.

  19. Enhancing science education through extracurricular activities: A retrospective study of "Suzy Science and the Whiz Kids(c)"

    Science.gov (United States)

    Kralina, Linda M.

    Extracurricular activities (ECA) are informal settings offering free-choice experiences that are generally voluntary, open-ended, non-sequential, self-directed, hands-on, and evaluation-free. This mixed methods study investigates participation in a high school science ECA by collecting the memories of former student members for their perceptions of engagement as well as social positioning. First, this study examines the levels in which the science club engaged these members, particularly females, in science and teaching. Second, the study also ascertains how participation in the club allowed members to explore new identities and fostered the development of new skills, actions and behaviors, expanding possible future trajectories of identification, specifically in science- and education-related career fields. Based on a review of the related literature regarding engagement and identity formation and the reconstructed reality from the memories of these students and sponsor, a theoretical framework has been constructed, based on seven essential elements of informal learning for an engaging as well as a socially constructive high school science ECA. The most significant findings are (1) the high correlation between engagement, specifically, cognitive engagement with social positioning, (2) the important role of emotional engagement in science ECA, (3) the major perception roadblocks to science learning that can be overcome, particularly for females in physical science, and (4) the importance of the teacher-student interactions in science ECA. Articulating a theoretical framework to legitimate the power of informal learning structures may help other educators to understand the potential benefits of science ECA and thus, increase opportunities for such experiential activities in order to enhance engagement and expand positioning of their students in science. More engaging, socially constructive science ECA have the potential to enhance science education.

  20. Building Self-Determination through Inclusive Extracurricular Programs

    Science.gov (United States)

    Vinoski, Erin; Graybill, Emily; Roach, Andrew

    2016-01-01

    Extracurricular activities provide students a range of rich experiences that influence their academic achievement, leadership and communication skills, and career paths. Students with disabilities (SWDs) historically have had limited access to extracurricular programs and thus fewer opportunities for academic, social, and vocational development.…

  1. What Do Mothers Say? Korean Mothers' Perceptions of Children's Participation in Extra-Curricular Musical Activities

    Science.gov (United States)

    Cho, Eun

    2015-01-01

    A recent study of Korean middle-class mothers' perceptions and parenting practices associated with children's participation in musical activities reported unique forms of musical parenting, which closely correspond with previous studies of concerted cultivation in Western middle-class families. Are these unique patterns exclusive to middle-class…

  2. What Do Mothers Say? Korean Mothers' Perceptions of Children's Participation in Extra-Curricular Musical Activities

    Science.gov (United States)

    Cho, Eun

    2015-01-01

    A recent study of Korean middle-class mothers' perceptions and parenting practices associated with children's participation in musical activities reported unique forms of musical parenting, which closely correspond with previous studies of concerted cultivation in Western middle-class families. Are these unique patterns exclusive to middle-class…

  3. Extracurricular Business Planning Competitions: Challenging the Assumptions

    Science.gov (United States)

    Watson, Kayleigh; McGowan, Pauric; Smith, Paul

    2014-01-01

    Business planning competitions [BPCs] are a commonly offered yet under-examined extracurricular activity. Given the extent of sceptical comment about business planning, this paper offers what the authors believe is a much-needed critical discussion of the assumptions that underpin the provision of such competitions. In doing so it is suggested…

  4. 大学生课外科技活动的探索与实践%Exploration and Practice on Extracurricular Scientific and Technological Activities of College Students

    Institute of Scientific and Technical Information of China (English)

    归风铁; 孙俊永; 冷向宇

    2012-01-01

    Based on the extracurricular scientific and technological activities actual of chemistry and chemical engineering majors,the analysis of the academic year thesis writing,community activities,organizing academic competitions,open laboratory,etc.was carried out.The mode,method reform measures of extracurricular scientific and technological activities reform practice on college students' extracurricular activities was discussed.It showed that the extracurricular scientific practice activities developed and exercised the unity and cooperation,teamwork and a good view of the world for the college students,and enhanced the competitiveness of the students in entrepreneurial employment.%结合本校化学化工类专业大学生课外科技活动实际,分析了学年论文写作、社团活动、举办学科竞赛、开放实验室等大学生课外科技活动的改革实践,讨论了大学生课外科技活动内容、模式、方法改革的主要措施。实践表明,课外科技实践活动培养和锻炼了大学生的团结协作能力、团队精神和良好的世界观,提升了学生在创业就业中的竞争力。

  5. Involvement in Physical Activity

    Directory of Open Access Journals (Sweden)

    James Gavin

    2013-04-01

    Full Text Available A total of 1,096 adolescents participated in 123 focus groups regarding the perceived outcomes of their involvement in sports and physical activity (PA. The groups, segmented by grade level, sex, and school types, were conducted in both public and private high schools in Montreal, Quebec. We sought to understand, through the participants’ own words, their perception of the outcome matrix of involvement in sports and PA. Focus group questions emphasized changes that adolescents associated with such engagement. In particular, participants were asked how sports and PA might influence behaviors, emotional states, personal characteristics, and other outcomes. Twelve themes were identified in the responses: Positive Health and Physical Changes (18.5%, Activity-Related Positive Emotions (15.6%, and Personal Learning (11.3% were most prevalent in the discussions. A cluster of deeper personal changes thematically described as Self-Identity, Autonomy, and Positive Character Development accounted for another 16.5% of the responses. Relatively few commentaries emphasized negative effects (7.1%. Converting the proportions of qualitative data into a quantitative index allowed us to analyze potential differences in emphasis according to sex, age, and school type. Though a few significant findings emerged, the larger pattern was of a uniform perceptual map across the variables for this adolescent sample. Implications drawn from this investigation highlight the need to clearly articulate concrete pathways to positive nonphysical changes (e.g., mood states, autonomy, positive character development from engagements in sports and PA.

  6. Research on College Students' Extracurricular Sports Activities Organization and Management Mode%普通高校大学生课外体育活动的组织管理模式研究

    Institute of Scientific and Technical Information of China (English)

    张凯

    2014-01-01

    Thia paper use of literature and questionnaires, site visits and other research methods, the status quo of college stu-dents in extra-curricular sports activities for development in Liaoning Province to investigate the characteristics and advan-tages and disadvantages of the current college extracurricular sports organization and management methods for analysis, summed up the impact of extracurricular activities factor in the development of extra-curricular sports activities of university organization and management model recommendations.%本文运用文献资料法和问卷调查、现场访问等研究方法,对辽宁省高校大学生课外体育活动发展现状进行调查,对目前高校课外体育活动组织管理方式的特点及利弊进行分析,总结出影响高校课外体育活动发展的因素,对高校课外体育活动的组织管理模式提出建议。

  7. Interações sociais de crianças pré-escolares com Síndrome de Down durante atividades extracurriculares Interacciones sociales de niños en edad preescolar con síndrome de Down durante actividades extracurriculares Social interactions of preschool children with Down syndrome during extracurricular activities

    OpenAIRE

    Renata Valdívia Lucisano; Luzia Iara Pfeifer; Maria Paula Panuncio Pinto; Jair Lício Ferreira Santos; Patrícia Páfaro Gomes Anhão

    2013-01-01

    O objetivo desta pesquisa foi identificar o processo de interação social de crianças com Síndrome de Down (SD) durante atividades extracurriculares na rede regular de educação infantil do município de Ribeirão Preto. Participaram da pesquisa seis crianças na faixa etária de 3 a 6 anos. Foram realizadas duas filmagens de cada criança em situações de interação social durante atividades extracurriculares, e analisadas através de quinze comportamentos, distribuídos em duas categorias de habilidad...

  8. Social Movement Oriented Citizenship in Colombia: The Effects of Curriculum, Pedagogy and Extra-Curricular Activities on Student Orientation

    Science.gov (United States)

    Edwards, D. Brent, Jr.

    2012-01-01

    Social movement oriented citizenship (SMOC) centers on peaceful protest, proactive community involvement and participation in activities to support human rights and environmental protection. Research generally on SMOC is extremely limited; even more so is research that analyses the influence of school- and student-level, policy-relevant variables…

  9. Analysis on Extracurricular Sports Activity in Middle School in Lishi Areas%离石区中学课外体育活动调查及分析

    Institute of Scientific and Technical Information of China (English)

    王福平

    2012-01-01

    主要运用问卷调查法对离石区中学生课外体育活动现状进行调查与分析,得出以下结论:离市区中学的学生不同程度地参加了体育活动,但锻炼的质量并不高;在参加课外体育活动时在活动项目形式的选择上都带有明显的性别和年龄特征;大部分学生参加课外体育活动的动机是较为积极的,认识是比较高的,但有少部分学生的活动动机存在问题,我们必须加以正确引导;影响中学生参加课外体育活动主要因素是:运动场所不足,师资力量的不足,运动器材短缺,国家投资力度的缺乏和对体育目的认识等。%Through using the method of questionnaire, this paper makes analysis on extracurricular sports activity of middle school students in Lishi areas. The result shows that middle school students participate in the sports ac- tivities at different degrees, but the exercise quality is not high. The selection of sports events is marked with gen- der and age characteristics in extracurricular sports activity. The motivation of most students participating in extra- curricular sports activity motivation is positive, but a small part of the student' s activity motivation exist problems. We must be correct guidance. The major factors affecting middle school students participating in extracur- ricular sports activities are lack of sports facilities and teachers, shortage of sports equipment, and lack of national investment and the awareness of sports purposes.

  10. Investigation on the Current Situation of Extracurricular Sports Activities of Middle School Students in Shanxi Province of Lucheng%山西省潞城一中学生课外体育活动现状调查研究

    Institute of Scientific and Technical Information of China (English)

    秦海风; 张金桥

    2014-01-01

    Using the questionnaire survey method, the extracurricular sports present situation and the influence factors of students of a middle school in Lucheng were investigated and analyzed. The results showed: the students on the whole to participate in extracurricular sports activities on the attitude is more positive; the number and time of students to participate in physical exercise less; students in the choice of sports, ball, jogging and aerobics are, and demonstrated a significant difference in sex; extracurricular sports activities in the main motivation is to enhance physical fitness, interest hobbies, entertainment; the main reason affecting students participating in extracurricular sports activities is the lack of facilities , academic stress, lack of exercise partner.%运用问卷调查等方法,对潞城一中学生课外体育活动现状及影响因素进行调查分析。结果显示:学生对参加课外体育活动的整体上态度较积极;学生每周参与体育锻炼的次数和时间偏少;学生在体育项目选择方面,以球类、跑步和健美操居多,且在性别上表现出显著差异;课外体育活动的动机主要是增强体质、兴趣爱好、消遣娱乐等;影响学生参加课外体育活动的主要原因是缺少场地器材,学业紧张,缺少运动伙伴等。

  11. Medical Students Participate in Extracurricular Sports Club Activities Investigation%某医科院校大学生参加课外体育社团活动现状调查

    Institute of Scientific and Technical Information of China (English)

    崔玖梅

    2011-01-01

    Objectives To explore the association of medical colleges and extracurricular sports activities related factors, the problems of objective assessment, provide recommendations for policy-making departments to promote the development of extra-curricular sports associations. Methods Literature review, questionnaire, in-depth inter-view, statistics and logical analysis and other methods were used for data collection and analysis. A Survey among university students participating in extracurricular sports associations a medical university in the west was carried out. Results A majority of college students are very more likely to participate in extracurricular or community activities. Exercise time to the evening of the main items in the choice of fitness, form, place an obvious gender characteristics of the time; students to participate in extracurricular sports organizations are motivated by multi-level,lack of sports facilities and the shortage of sports equipment are the main factors to influence students' participating in extracurricular sports associations. Conclusions Strengthening the management and guidance to extracurricular sports club activities have an important role in carrying out the work of sports and sports cultural construction of medical institutions.%目的 探究影响医学院校课外体育社团活动的相关因素,对存在的问题进行客观的评价,为决策部门提供建议,以促进课外体育社团的发展.方法 通过文献资料法、问卷调查法、访谈法、数理统计法和逻辑分析法等方法,对西部某医科院校大学学生参加课外体育社团情况进行现状调查.结果 大部分大学生喜欢或非常喜欢不同程度地参加课外体育社团活动.锻炼的时间以晚上为主,在选择健身项目、形式、场所时带有明显的性别特征;学生参加课外体育社团的动机是多层次的;运动场所的不足和运动器材的短缺是影响学生参加课外体育社团

  12. Promoting Active Involvement in Classrooms

    Science.gov (United States)

    Conderman, Greg; Bresnahan, Val; Hedin, Laura

    2012-01-01

    This article presents a rationale for using active involvement techniques, describes large- and small-group methods based on their documented effectiveness and applicability to K-12 classrooms, and illustrates their use. These approaches include ways of engaging students in large groups (e.g., unison responses, response cards, dry-erase boards,…

  13. "If You Want to Win, You Have to Learn to Get Along:" Youth Talk about Their Participation in Extracurricular Activities

    Science.gov (United States)

    Dworkin, Jodi; Bremer, Karin Lindstrom

    2004-01-01

    Young people report being both emotionally and cognitively engaged in youth activities in ways they are not in other parts of their lives. Research has identified six developmental processes that categorize youths' growth experiences in their activities. These include identity work; developing initiative; learning, emotional competencies;…

  14. 略论普通高校学生课外体育活动的组织管理%On Organization and Management of College Extracurricular Sports Activities

    Institute of Scientific and Technical Information of China (English)

    严华; 李蓉蓉

    2012-01-01

    The essay analyzes and discusses the management model of college students' extracurricular sports activities,way of organization and the content arrangement,and then proposes some measures to carry out these extra-curricular sports activities on the basis of a comprehensive analysis of the management of these activities,the current situation of the students' participation in the activities and the objective and subjective factors which constrain the development of these activities.%在全面分析高校对课外活动的管理、学生参与课外活动的现状以及制约课外活动开展的主客观因素的基础上,结合实际,对高校学生课外体育活动管理模式、组织方式、内容安排等进行了分析和讨论,提出积极开展高校课外体育活动的对策措施。

  15. Randomised feasibility trial of a teaching assistant led extracurricular physical activity intervention for 9 to 11 year olds: Action 3:30.

    Science.gov (United States)

    Jago, Russell; Sebire, Simon J; Davies, Ben; Wood, Lesley; Edwards, Mark J; Banfield, Kathryn; Fox, Kenneth R; Thompson, Janice L; Powell, Jane E; Montgomery, Alan A

    2014-09-11

    Extracurricular programmes could provide a mechanism to increase the physical activity (PA) of primary-school-aged children. The aim of this feasibility study was to examine whether the Action 3:30 intervention, which is delivered by teaching assistants, holds promise as a means of increasing the PA of Year 5 and 6 children. A cluster randomised feasibility trial was conducted in 20 primary schools. Ten schools received the Action 3:30 intervention and 10 schools were allocated to the control arm. The intervention was 40 one-hour sessions, delivered twice a week by teaching assistants. The proportion of participants recruited per school was calculated. Session delivery and session attendance was calculated for intervention schools. Weekday and after-school (3.30 to 8.30 pm) moderate to vigorous intensity physical (MVPA) was assessed by accelerometer at baseline (T0), during the last few weeks of the intervention (T1) and four months after the intervention had ended (T2). The costs of delivering the intervention were estimated. Five intervention schools ran all 40 of the intended sessions. Of the remaining five, three ran 39, one ran 38 and one ran 29 sessions. Mean attendance was 53%. The adjusted difference in weekday MVPA at T1 was 4.3 minutes (95% CI -2.6 to 11.3). Sex-stratified analyses indicated that boys obtained 8.6 more minutes of weekday MVPA than the control group (95% CI 2.8 to 14.5) at T1 with no effect for girls (0.15 minutes, 95% CI -9.7 to 10.0). There was no evidence that participation in the programme increased MVPA once the club sessions ceased (T2). The indicative average cost of this intervention was £2,425 per school or £81 per participating child during its first year and £1,461 per school or £49 per participating child thereafter. The effect of the Action 3:30 intervention was comparable to previous physical activity interventions but further analysis indicated that there was a marked sex difference with a positive impact on boys and no

  16. Understanding affluent adolescent adjustment: The interplay of parental perfectionism, perceived parental pressure, and organized activity involvement.

    Science.gov (United States)

    Randall, Edin T; Bohnert, Amy M; Travers, Lea V

    2015-06-01

    This cross-sectional study examined relations between affluent adolescent adjustment and culturally salient factors within parent-child relationship and extracurricular domain. Bootstrapping techniques evaluated mediated effects among parental perfectionism, perceived parental pressure, intensity of organized activity (OA) involvement, and adolescent adjustment (i.e., depressive and anxiety symptoms, life satisfaction) within a sample of 10th graders and their parents (n = 88 parent-child pairs) from four high schools in affluent communities. Findings indicated that adolescents with more perfectionistic parents perceived more parental pressure and experienced poorer adjustment. Results also demonstrated that affluent adolescents who perceived more parental pressure were more intensely involved in OAs, but that higher OA intensity was linked to better adjustment. Findings highlight the importance of considering parental perfectionism when understanding adolescent behaviors and psychological outcomes, confirm the negative direct effects of parental pressure on adjustment, and corroborate prior research dispelling that highly intense OA involvement is linked to adolescent maladjustment.

  17. Modeling interdisciplinary activities involving Mathematics

    DEFF Research Database (Denmark)

    Iversen, Steffen Møllegaard

    2006-01-01

    In this paper a didactical model is presented. The goal of the model is to work as a didactical tool, or conceptual frame, for developing, carrying through and evaluating interdisciplinary activities involving the subject of mathematics and philosophy in the high schools. Through the terms...... domains (Michelsen, 2001, 2005a, 2005b). Furthermore the theoretical description rest on a series of qualitative interviews with teachers from the Danish high school (grades 9-11) conducted recently. The special case of concrete interdisciplinary activities between mathematics and philosophy is also...

  18. Extracurricular school-based sports as a motivating vehicle for sports participation in youth: a cross-sectional study.

    Science.gov (United States)

    De Meester, An; Aelterman, Nathalie; Cardon, Greet; De Bourdeaudhuij, Ilse; Haerens, Leen

    2014-04-07

    Extracurricular school-based sports are considered to be an ideal means of reaching children who are not active in community sports. The purposes of this study were to examine the extent to which pupils not engaging in community sports do participate in extracurricular school-based sports, and to assess whether extracurricular school-based sports participants are more physically active and/or more autonomously motivated towards sports in daily life than children who do not participate in extracurricular school-based sports. One thousand forty-nine children (53.7% boys; M age = 11.02 years, SD = 0.02) out of 60 classes from 30 Flemish elementary schools, with an extracurricular school-based sports offer, completed validated questionnaires to assess physical activity (Flemish Physical Activity Questionnaire) and motivation (Behavioral Regulations in Physical Education Questionnaire). Multilevel regression analyses were conducted to examine the data generated from these questionnaires. More than three quarters of the children (76%) reported participating in extracurricular school-based sports during the current school year and 73% reported engaging in organized community sports. Almost two third of the children (65%) not participating in community sports stated that they did participate in extracurricular school-based sports. Extracurricular school-based sports participants were significantly more physically active than children not participating in extracurricular school-based sports (β = 157.62, p sports participation × community sports participation) were found for autonomous motivation, with boys engaging in extracurricular school-based sports but not in community sports being significantly more autonomously motivated towards sports than boys not engaging in community or extracurricular school-based sports (β = 0.58, p = 0.003). Such differences were not noted among girls. If extracurricular school-based sports are offered at school, the vast majority of

  19. Modeling interdisciplinary activities involving Mathematics

    DEFF Research Database (Denmark)

    Iversen, Steffen Møllegaard

    2006-01-01

    In this paper a didactical model is presented. The goal of the model is to work as a didactical tool, or conceptual frame, for developing, carrying through and evaluating interdisciplinary activities involving the subject of mathematics and philosophy in the high schools. Through the terms...... of Horizontal Intertwining, Vertical Structuring and Horizontal Propagation the model consists of three phases, each considering different aspects of the nature of interdisciplinary activities. The theoretical modelling is inspired by work which focuses on the students abilities to concept formation in expanded...... domains (Michelsen, 2001, 2005a, 2005b). Furthermore the theoretical description rest on a series of qualitative interviews with teachers from the Danish high school (grades 9-11) conducted recently. The special case of concrete interdisciplinary activities between mathematics and philosophy is also...

  20. 基于制造业的高职职业英语第二课堂教学研究%A Study on the Teaching Strategies of EOP in Extracurricular Activities Based on Manufacturing in Vocational Colleges

    Institute of Scientific and Technical Information of China (English)

    张妍

    2014-01-01

    Manufacturing is the basic industry of national defense as well as economic progress. Under the backgrounds of dynamization, customization and globalization, manufacturing and services have an increasingly closer relationship in development. Improving service quality plays a vital role in manufacturing in China,and EOP in extracurricular activities is one of the necessary media to realize the goal. Vocational colleges located in Northeast China are obliged to do researches for achieving the aim of“Made in China”in the servitization of manufacturing. This paper studies on the main idea of EOP,the present state of English extracurricular activities, and the teaching strategies of EOP in extracurricular activities on the basis of manufacturing and modern service consciousness including local features, industry trend,contest participation,teacher-student relationship,ability development and guarantee mechanism.%制造业是国防建设和经济发展的基础产业。在全球顾客需求动态化、定制化、国际化背景下,制造业与服务业日趋融合发展,提高服务质量对于我国制造业至关重要,而职业英语(EOP)第二课堂是实现这一愿景的必要载体。东北地区的高职院校有责任探索在服务化转型的过程中成就“中国制造”的方略。通过分析EOP内涵,结合高职第二课堂开展现状,从地域特征、行业走向、以赛促学、师生角色、能力养成、保障机制等方面研究基于制造业且具有现代服务意识的高职EOP第二课堂教学策略。

  1. A Study on Extracurricular Activities of College English Learning in the New Media Environment%新媒体环境下大学英语课外活动研究

    Institute of Scientific and Technical Information of China (English)

    金丽

    2016-01-01

    The booming of modern information technology has created a better language environment for college students to learn English after class. College English teachers should design extracurricular activities scientifically by making good use of various kinds of learn⁃ing platform and improve students’ language ability by combining language input and language output activities.%现代信息技术飞速发展,客观上为大学英语课外学习创造了一个良好的环境。教师应充分利用新媒体搭建各种平台,科学合理设计课外学习活动,将语言输入与输出有效结合,从而全面提高学生的语言交际能力。

  2. Research on the Construction of Extracurricular Activities of College Students' Ideological and Political Education at Different Levels%分层分级大学生课外思想政治教育活动的构建研究

    Institute of Scientific and Technical Information of China (English)

    张丹; 柏杨

    2011-01-01

    Constructing extracurricular activities of college students' ideological and political education at different levels is a trend to promote college students' overall development in both commonness and individuality. It is not only an important carrier to foster innovative talents with the concept of people-orientation and scientific development, but also an inevitable approach to build socialist harmonious society. To build such a system, it is necessary to deepen the construction of campus culture, enrich the activities of students' social practice, strengthen the construction of the Party and League organization. Along with an interactive platform of new campus media, and the mental health education and career planning education, efforts are to be made on constructing extracurricular activities of college students' ideological and political education at different levels.%构建分层分级大学生课外思想政治教育活动,是实现大学生共性和个性全面发展的必然趋势。不仅能体现以人为本、科学发展的理念,是培养创新型人才的的重要载体,更是构建社会主义和谐社会的必然要求。构建这样的体系,必须深化大学校园文化建设,丰富大学生社会实践活动形式,加强党团组织建设,建设校园新媒体互动平台,健全心理健康教育和职业生涯规划教育,打造分层分级大学生课外思想政治教育工作合力。

  3. Applying a Contest to Improve Learning in the Information Systems Development - An Interdisciplinary and Extracurricular Approach

    Directory of Open Access Journals (Sweden)

    Marcelo FANTINATO

    2011-10-01

    Full Text Available Contests are usually applied in the academic environment to simulate real professional situations that require from the participants a more pro-active attitude than the one shown in conventional coursework. Although they are commonly applied in the scope of a unique course, the contest described here was an extracurricular experience applied in an Information System undergraduate program. The evaluation of the contest is also presented; the objective was to assess the role of the contest as a tool to bring together interdisciplinary subjects, complementary to the traditional disciplinary structure of the program curriculum. The results indicate that a significant portion of the participants noticed increase in their knowledge after the contest, which is verified by statistical tests. However, students from the first stages received more benefits, probably because such students were more motivated and had more available time to be involved in the contest activities.

  4. A Survey on the Present Situation of Rural Pupils’Extracurricular Activities in the West:A Case Study on a Certain Primary School in Gansu Province%西部农村小学课外活动现状调查研究——以甘肃省某小学为例

    Institute of Scientific and Technical Information of China (English)

    周莉莉

    2012-01-01

    为了了解西部农村小学课外活动的现状,本研究采用调查研究法对甘肃省某农村小学的学生进行调查,调查的结果是:西部农村小学课外活动种类单一,内容贫乏;活动经费不足;缺少安全保障;缺乏有效的组织管理;家长和教师对课外活动缺乏正确的认识。针对这些问题,本研究从加大经费的投入、转变教师和家长的认识、加强课外活动的组织和管理的角度,提出对策建议。%In order to understand the present situation of extra-curricular activities in primary schools in rural areas in the west, this study used survey research methods to investigate a rural primary school students in Gansu province. Survey result:Western rural elementary school extra-curricular activity types single,devoid of matter;Inadequate funding for activities;Lack of safety and security; Lack of effective organization management; Lack of proper understanding of extra-curricular activities for parents and teachers.On the issues,the study from the investment of increased funding, changing teachers and parents awareness, strengthen extra-curricular activities of the Organization and management point of view, put forward suggestions.

  5. The Status Quo on Participation in Extracurricular Sports Activities for Vocational College Students%浅析高职院校学生参与课外体育活动的现状

    Institute of Scientific and Technical Information of China (English)

    赵红艳

    2012-01-01

    Based on the first-year students from five vocational colleges in Tianjin Haihe Educational Park,a survey and analysis on participation in sports activities outside school hours is conducted by questionnaire and mathematical statistics.According to the survey results,it is recommended that colleges start from the increase in sport funding,the reform of physical education and sports management,and actively strengthen the students’ extracurricular sports activities.%以天津市海河教育园五所高职的大一学生为研究对象,采用问卷调查法、数理统计法对五所高职部分大一学生在课外时间里参与体育活动的情况进行了调查和分析。依据调查结果,建议学校从增加体育经费、改革体育教学和加强体育管理等方面入手,积极加强学生课外体育活动工作。

  6. Parental exercise is associated with Australian children's extracurricular sports participation and cardiorespiratory fitness: A cross-sectional study

    Directory of Open Access Journals (Sweden)

    Dwyer Terence

    2005-04-01

    Full Text Available Abstract Background The relationship between parental physical activity and children's physical activity and cardiorespiratory fitness has not been well studied in the Australian context. Given the increasing focus on physical activity and childhood obesity, it is important to understand correlates of children's physical activity. This study aimed to investigate whether parental exercise was associated with children's extracurricular sports participation and cardiorespiratory fitness. Methods The data were drawn from a nationally representative sample (n = 8,484 of 7–15 year old Australian schoolchildren, surveyed as part of the Australian Schools Health and Fitness Survey in 1985. A subset of 5,929 children aged 9–15 years reported their participation in extracurricular sports and their parents' exercise. Cardiorespiratory fitness was measured using the 1.6 km (1-mile run/walk and in addition for children aged 9, 12 or 15 years, using a physical work capacity test (PWC170. Results While the magnitude of the differences were small, parental exercise was positively associated with children's extracurricular sports participation (p p 170 (p = 0.013. In most instances, when only one parent was active, the sex of that parent was not an independent predictor of the child's extracurricular sports participation and cardiorespiratory fitness. Conclusion Parental exercise may influence their children's participation in extracurricular sports and their cardiorespiratory fitness levels. Understanding the correlates of children's extracurricular sport participation is important for the targeting of health promotion and public health interventions, and may influence children's future health status.

  7. Extra-Curricular and out-of-School Education in European Socialist Countries.

    Science.gov (United States)

    Wegerich, Hans-Joachim

    1988-01-01

    Describes extra-curricular activities in East Germany, Bulgaria, Czechoslovakia, Poland, Romania, and the USSR, and provides an annotated bibliography that covers activities in the social sciences; science and technology; natural sciences; arts and culture; sports; tourism; mass media; after school centers; holiday activities; and youth…

  8. “以学生为中心”的课外科技活动在提升中医药院校大学生创新能力的探索%Application of the“Student-centered” Extracurricular Activities of Science and Technology in the Traditional Chinese Medicine Colleges and Universities to Improve the Ability of Innovation

    Institute of Scientific and Technical Information of China (English)

    李玲; 刘慧萍; 喻嵘; 何清湖; 谭佳佳; 张银银; 杨仁义

    2016-01-01

    “以学生为中心”的课外科技活动旨在提供给大学生一个拓宽不同科学领域的平台,帮助大学生合理利用课外时间、培养创新精神、提高科研实践能力,有助于中医药院校大学生成为当下社会需求的综合性高素质人才。我校利用学生课外科技活动这一重要途径,通过设立校级大学生研究性学习和创新性实验计划项目,构建多级别多形式的课外科技活动体系和建立健全大学生课外科技活动运行管理机制等具体措施,积极组织中医药本科学生开展课外科技活动,在学生科研技能、实践能力、专业水平、人文素养、创新精神和创新能力等方面取得了较好效果。%“Students‐centered” extracurricular activities of science and technology is the effective means to cultivate the spirit of innovation and practical abilities of college students ,plays an important role in the growth of college students in Colleges and universities of Chinese medicine .My school extra‐curricular science and technology activities by way of this important ,through the establishment of the college students’ research learning and innovative experiment project ,construction of school ,prov‐ince ,three levels of extracurricular activities in science and technology and the establishment of a sound system of national college students extracurricular activities of science and technology manage‐ment system and other specific measures , and actively organize Chinese medicine undergraduate students extracurricular scientific research the work ,carried on the beneficial exploration to improve the comprehensive quality of students ,and achieved good results .Through the vertical and horizontal comparison ,medical undergraduates participated in extracurricular scientific activities in all aspects of students’ scientific research quality ,practical ability ,professional quality ,humanistic quality ,inno

  9. Supporting Active User Involvment in Prototyping

    DEFF Research Database (Denmark)

    Grønbæk, Kaj

    1990-01-01

    in the development process. But prototyping does not automatically imply active user involvement! Thus a cooperative prototyping approach aiming at involving users actively and creatively in system design is proposed in this paper. The key point of the approach is to involve users in activities that closely couple......The term prototyping has in recent years become a buzzword in both research and practice of system design due to a number of claimed advantages of prototyping techniques over traditional specification techniques. In particular it is often stated that prototyping facilitates the users' involvement...... development of prototypes to early evaluation of prototypes in envisioned use situations. Having users involved in such activities creates new requirements for tool support. Tools that support direct manipulation of prototypes and simulation of behaviour have shown promise for cooperative prototyping...

  10. Pupils' involvement in ecological activities in school

    Directory of Open Access Journals (Sweden)

    Stanišić Jelena

    2009-01-01

    Full Text Available Ecological education gains an increasing importance in the moment when ecological crisis acquires increasingly larger proportions. Education is one of the most powerful social means in fighting problems and challenges of the future. This paper presents the results of the research referring to pupils' attitudes with respect to the activities they prefer to be involved in, the independent variables their readiness for involvement depends on, as well as the relation between the knowledge possessed by pupils and their readiness to be involved in ecological activities. Research was conducted on the sample of 284 pupils in the eighth grade of primary school in urban and rural area and polluted and unpolluted environment. The results indicate that pupils find more interesting the activities taking place in nature, those where they are immediate participants and those where they have a subjective feeling that they are doing something to protect the environment. Besides, the results show that girls from both samples and pupils from rural area are more ready to get involved in ecological activities. Living in polluted or unpolluted environment is not a statistically significant variable which would influence the readiness of pupils to get involved in these activities. As the most interesting result of this research, we consider the finding that a higher level of pupils' ecological knowledge and living in polluted environment do not guarantee higher readiness of pupils to get involved in the activities of environment protection.

  11. Extracurricular Training in Informatics in Ruse

    Directory of Open Access Journals (Sweden)

    Katalina GRIGOROVA

    2010-10-01

    Full Text Available The authors underline their opinion that it is preferable to organise the extracurricular education on city level instead of forming autonomous school groups. Putting together students from different schools forces them to share their knowledge and programming techniques. All this helps them get familiar with new practices and experience and inspires ambitions for growth and progress.

  12. Extracurricular Training in Informatics in Ruse

    Science.gov (United States)

    Grigorova, Katalina; Hristova, Plamenka

    2010-01-01

    The authors underline their opinion that it is preferable to organise the extracurricular education on city level instead of forming autonomous school groups. Putting together students from different schools forces them to share their knowledge and programming techniques. All this helps them get familiar with new practices and experience and…

  13. Learning models of activities involving interacting objects

    DEFF Research Database (Denmark)

    Manfredotti, Cristina; Pedersen, Kim Steenstrup; Hamilton, Howard J.;

    2013-01-01

    We propose the LEMAIO multi-layer framework, which makes use of hierarchical abstraction to learn models for activities involving multiple interacting objects from time sequences of data concerning the individual objects. Experiments in the sea navigation domain yielded learned models that were...... then successfully applied to activity recognition, activity simulation and multi-target tracking. Our method compares favourably with respect to previously reported results using Hidden Markov Models and Relational Particle Filtering....

  14. Investigation to the Status Quo of the Extracurricular Football Activity Carrying out in Shangqiu Normal University%商丘师范学院课外足球运动开展状况调查分析

    Institute of Scientific and Technical Information of China (English)

    朱万文

    2016-01-01

    This paper using methods of literature materials, investigation, mathematical statistics and logi⁃cal reasoning, conducts a research on the existing problems the current extracurricular football activity in Shangqiu normal university, points out that more attention should be paid to the development of extracurric⁃ular football, the teacher's level should be improved, the construction of the stadium facility should be in⁃creased, a football association should be formed to organize football games actively, to make more students participate in football, futsal competition should be popularized, the utilization rate of field should be im⁃proved, students’enthusiasm for football should be promoted and their skills and tactics should be im⁃proved.%运用文献资料法、调查法、数理统计法等研究方法,以商丘师范学院课外足球活动的开展为研究对象,对课外足球活动开展现状以及存在问题进行研究。提出商丘师范学院学应重视课外足球的发展,提高教师水平并加大场地设施的建设,成立足球协会积极组织足球比赛,使更多的学生参与到足球运动中来,推广五人制足球,提高场地利用率,促进学生对足球运动的热情和自身技战术的提高。

  15. The Work and Role of Extracurricular Clubs in Fostering Student Creativity

    Science.gov (United States)

    Kuhar, Kristina; Sabljic, Jakov

    2016-01-01

    Extracurricular activities play an important role in the educational process as they provide students with the opportunity to deepen their knowledge and develop various skills, meet their creative needs and acquire the culture of free time. They also provide space for a closer relationship between the club coordinators and students, for students'…

  16. Youth Activity Involvement, Neighborhood Adult Support, Individual Decision Making Skills, and Early Adolescent Delinquent Behaviors: Testing a Conceptual Model

    Science.gov (United States)

    Crean, Hugh F.

    2012-01-01

    This study examines a cross-sectional structural equation model of participation in youth activities, neighborhood adult support, individual decision making skills, and delinquent behavior in urban middle school youths (n = 2611). Results indicate extracurricular activity participation had both direct and indirect associations with delinquent…

  17. Supporting Active User Involvment in Prototyping

    DEFF Research Database (Denmark)

    Grønbæk, Kaj

    1990-01-01

    development of prototypes to early evaluation of prototypes in envisioned use situations. Having users involved in such activities creates new requirements for tool support. Tools that support direct manipulation of prototypes and simulation of behaviour have shown promise for cooperative prototyping...... activities. Examples of such tools are given and the ways that they support cooperative prototyping within various use domains are discussed.......The term prototyping has in recent years become a buzzword in both research and practice of system design due to a number of claimed advantages of prototyping techniques over traditional specification techniques. In particular it is often stated that prototyping facilitates the users' involvement...

  18. Learning models of activities involving interacting objects

    DEFF Research Database (Denmark)

    Manfredotti, Cristina; Pedersen, Kim Steenstrup; Hamilton, Howard J.;

    2013-01-01

    We propose the LEMAIO multi-layer framework, which makes use of hierarchical abstraction to learn models for activities involving multiple interacting objects from time sequences of data concerning the individual objects. Experiments in the sea navigation domain yielded learned models that were...

  19. Learning models of activities involving interacting objects

    DEFF Research Database (Denmark)

    Manfredotti, Cristina; Pedersen, Kim Steenstrup; Hamilton, Howard J.

    2013-01-01

    We propose the LEMAIO multi-layer framework, which makes use of hierarchical abstraction to learn models for activities involving multiple interacting objects from time sequences of data concerning the individual objects. Experiments in the sea navigation domain yielded learned models that were t...

  20. The Country Council Work on Extracurricular Enlightenment of Adults in the Volga-Ural Region

    Directory of Open Access Journals (Sweden)

    Timur A. Magsumov

    2015-12-01

    Full Text Available The article reveals the activity of the Volga and Ural country councils to create a system of extracurricular enlightenment of adults in the later period of the existence of the Russian empire. The article is based on the statistical and mass sources, including office records from regional archives. The authors, resorting to a comparative multiaspect approach to the study of the country councils’ forms and methods of work to organize extracurricular establishments and popular educational events, make an overview of tendencies and directions, progress and problems in their development. The belated formation of the extracurricular adult education system the authors explain by the country councils; focus on primary education. The study revealed the dominant poison of country councils in the sphere of extracurricular enlightenment, a financial and pedagogical availability of this education form for great masses of population of this education form, combining general knowledge and professional skills. The social and cultural activity of country councils in the late XIXth – early XXth c.c. was profound and diverse as far as enlightenment is concerned, and helped achieve top results within a short period of time.

  1. 中国和瑞典高中生学习负担与课外活动的比较研究%A Comparative Investigation on the Learning Stress and Extracurricular Activities Between Senior High School Students in China and Sweden

    Institute of Scientific and Technical Information of China (English)

    陈传锋; 杨娜; Janine Sages

    2014-01-01

    The learning stress and extracurricular activities for high school students are affected by national economy,culture and social development.Learning about foreign senior high students ’ learning stress and extracurricular activities has important referential significance in reflecting and improving the learning and life situation for the senior high school students in our country. Therefore,this paper,by means of questionnaire investigation and interview method,investigates the situation in learning stress and extracurricular activities between the senor high school students in the city of Ningbo and Sweden,and the result shows that there exist apparent differences between the students of different countries in time table,in class and after class learning,homework and exam,outside school leisure time activities and mental state,namely,the learning stress for the senor high school students in our country is higher than that in Sweden and the extracurricular activities are obviously more monotonous than those in Sweden.%中学生的学习负担与课余活动受国家的经济、文化和社会发展的影响,了解国外高中生的学习负担与课余活动,对反思和改善我国高中生的学习与生活状况具有重要的借鉴意义。因此,运用问卷调查法和访谈法,对我国宁波市和瑞典隆德市高中生的学习负担与课余活动进行了调查,比较分析结果显示:两国高中生在作息时间、课内外学习、学习兴趣、作业考试、校外休闲活动和心理状态等维度上存在显著差异,亦即,我国高中生的学习负担显著高于瑞典高中生,而课余活动明显比瑞典高中生单调。

  2. Research of the Participating in Extracurricular Sports Activities of the Only-Child Common College Students-Take Suzhou University as an Example%独生子女大学生参加课余体育活动现状研究--以宿州学院为例

    Institute of Scientific and Technical Information of China (English)

    田慧丽

    2016-01-01

    The actual situation of Suzhou University students to participate in extra-curricular sports activities are studied by using the method of literature,questionnaire survey method,mathematical statistics method.The re-sults show that these students have different attitudes and motives when they participating extracurricular sports ac-tivities.We find out the subjective and objective factors that affecting their participation in extracurricular sports ac-tivities according to the psychological characteristics of the only-child college students.College should give special care to this group from many aspects.%本文通过文献资料法,问卷调查法,数理统计法等方法研究宿州学院独生子女大学生参加课余体育活动的实际情况以及出现的问题,结果显示:该群体从事课外体育活动的态度和动机各有不一,总的来说意识都相对薄弱,想法和行动存在较大偏差;课余体育活动的参与时间、频度以及方式也存在差异。根据现阶段独生子女的心理特点,找出了影响该群体参与课外体育活动的主客观因素,并提出高校应当从思想和行动等多方面给予该群体大学生特殊关怀等合理化建议。

  3. Helping General Physical Educators and Adapted Physical Educators Address the Office of Civil Rights Dear Colleague Guidance Letter: Part II--What Parents Should Know and Do to Ensure Extracurricular Interscholastic Sport Opportunities for Their Children with Disabilities

    Science.gov (United States)

    Tymeson, Garth

    2013-01-01

    The rights of all students to participate in extracurricular programs are required by current law but are often nonexistent, misunderstood, or even sometimes purposely not shared with parents. This article is addressed to parents regarding the rights of their children with disabilities to participate in extracurricular sport activities. Included…

  4. Helping General Physical Educators and Adapted Physical Educators Address the Office of Civil Rights Dear Colleague Guidance Letter: Part V--Outdoor Pursuits as an Extracurricular Alternative for Addressing Office of Civil Rights Guidance

    Science.gov (United States)

    Davis, Timothy D.; Felix, Manny

    2013-01-01

    The Office of Civil Rights (OCR) recently clarified that schools are required to provide students with disabilities (SWD) equal opportunities to participate in extracurricular activities (U.S. Department of Education [USDE] Office for Civil Rights, 2013). Schools have flexibility to design and expand extracurricular opportunities based on existing…

  5. Adolescent girls' and parents' views on recruiting and retaining girls into an after-school dance intervention: implications for extra-curricular physical activity provision

    Directory of Open Access Journals (Sweden)

    Powell Jane

    2011-08-01

    Full Text Available Abstract Background Many adolescents are not sufficiently active and girls are less active than boys. Physical activity interventions delivered during curriculum time have reported weak effects. More sustained changes in physical activity may be obtained by facilitating participation in enjoyable activities. Dance is the favourite activity of UK girls but there is a shortage of dance provision. Dance sessions delivered after the school day could prove to be an effective means of engaging adolescent girls in physical activity. There is a lack of information about the factors that would affect girls' recruitment and retention in an after-school dance programme. Methods Focus groups were conducted with 65, Year 7 (11-12 year old girls from 4 secondary schools in Bristol. In-depth phone interviews were also conducted with 16 (4 per school of the girls' parents. Interviews and focus groups examined issues that would affect recruitment into the intervention, strategies that could be used to attract girls who have little or no previous experience in dance, any factors that would increase their interest in participating in an after-school dance programme and any factors that would affect retention in the programme. All interviews and focus groups were digitally recorded and thematically analysed. Results Girls reported that a taster session in which they had an opportunity to sample the intervention content and "word of mouth" campaigns by peers, who did not need to be their friends, would encourage them to participate in an after-school dance programme. Sessions that maximised enjoyment and facilitated socialisation opportunities would enhance retention. Parents reported that encouraging groups of friends to join the programme, and stressing the enjoyment of the session would increase participation. Conclusions Recruitment and retention campaigns that focus on enjoyment, socialisation, mastery, goal setting and relating to other girls may be effective

  6. School-Based Extracurricular Astronomy

    Science.gov (United States)

    Stanger, Jeffrey J.

    2010-01-01

    The International Year of Astronomy in 2009 focused considerable public attention on Astronomy and generated valuable resources for educators. These activities are an effective vehicle for promoting Science to students and to the wider school community. The most engaging practical astronomy activities are best delivered with sustained support from…

  7. School-Based Extracurricular Astronomy

    Science.gov (United States)

    Stanger, Jeffrey J.

    2010-01-01

    The International Year of Astronomy in 2009 focused considerable public attention on Astronomy and generated valuable resources for educators. These activities are an effective vehicle for promoting Science to students and to the wider school community. The most engaging practical astronomy activities are best delivered with sustained support from…

  8. Teaching Astronomy in Extracurricular Study Groups of Armenia

    Science.gov (United States)

    Khachatryan, Mher; Grigoryan, Avetik

    2016-12-01

    The report presents the history of activity of Extracurricular Study Groups of Ar¬menia teaching astronomy and related subjects. It mainly refers to the Aerospace Club founded in 1988, which has long been acting as an officially unre¬gis¬tered, but efficiently performing non-governmental organization - Armenian Youth Ae¬ro¬space Society. The Club teaches, provides a truly scientific view of the world, advocates astronomy and other scientific and technical areas, provides interesting lectures and ar¬ticles to schools and mass media, arranges seminars and meetings with renowned experts, publishes scientific ar¬ticles, manuals, books, puts forward important scientific and techno-logical problems and offer students to work together on them, seek for solutions and develop possible appli¬ca¬tions. All this is aimed at maintaining and further development of leading positions of Armenia's scientific potential, particularly in astronomy.

  9. 挖掘学生潜力,实现教学最优化--信息时代背景下对大学英语第二课堂的探究%The Optimization of Teaching by Digging out Students’ potentiality---Exploration of College English Extracurricular Activities in Information Age

    Institute of Scientific and Technical Information of China (English)

    欧旦阳

    2014-01-01

    随着信息时代的到来,社会对大学生的综合素质要求越来越高。高校大力发展英语第二课堂,充分调动大学生学习英语的积极性和自主性,挖掘学生潜力成为实现教学最优化的必经之路。而在信息时代的新形势下,网络第二课堂与有效的、传统的第二课堂相结合,即融多主题英语实践活动、校园英文广播和网络学习为一体的混合式教学才是培养具有自主学习能力、建构能力、创新能力的应用型人才的有效途径。%With the coming of Information Age,society needs graduates with comprehensive skills to build it.It is a necessity that college makes full use of English extracurricular activities to inspire students to study autonomic learning,digging out their potentiality,thus optimizing English teaching. In Information Age where communication looms large,an efficient way of raising creative talent with construction competency is to combine the excellent traditional college English extracurricular activities with the modern network class, which is, the blended teaching approach of multi-topic English activities,campus radio and online study.

  10. Measuring psychological engagement in youth activity involvement.

    Science.gov (United States)

    Ramey, Heather L; Rose-Krasnor, Linda; Busseri, Michael A; Gadbois, Shannon; Bowker, Anne; Findlay, Leanne

    2015-12-01

    Although psychological engagement (e.g., enjoyment, concentration) may be critical in fostering positive outcomes of youth activity participation, too few studies have been conducted to establish its role in development. Furthermore, an established measurement tool is lacking. In the current study, we evaluated a brief engagement measure with two Canadian samples of youth (Sample 1, N = 290, mean age = 16.9 years, 62% female; Sample 2, N = 1827, mean age = 13.1 years, 54% female). We conducted a confirmatory factor analysis with structural equation modeling to examine the hypothesized structure of the model. We also assessed the measure's validity by testing relations between engagement and both perceived outcomes and positive features of activity settings. Psychological engagement was best captured by three latent cognitive, affective, and relational/spiritual factors and a second-order latent factor. Also, as anticipated, psychological engagement was associated with features of the activity setting and perceived impact.

  11. A Study of the Influencing Factors of English Extracurricular Reading for Junior Middle School Students

    Institute of Scientific and Technical Information of China (English)

    张金盼

    2015-01-01

    Extracurricular reading as an extension of the junior middle school English teaching process,which can broaden the students’ horizons.In this dissertation,the influencing factors of the junior high school students’ extracurricular reading are described.According to these factors,corresponding improving measures are put forward to improve the level of learning English through English extracurricular reading.

  12. Improvement of physical culture among children and youth in extracurricular education institutions

    Directory of Open Access Journals (Sweden)

    Natalia Tikhonova

    2014-10-01

    Full Text Available Purpose: is to improve the physical culture extracurricular education institutions. Materials and Methods: the study employed a range of scientific methods, including literary sources and documents analysis; expert evaluation method; methods of system analysis; sociological methods; statistical methods, pedagogical experiment. The study involved 20 managers, 120 coaches from sport organizations, as well as 4 managers of physical culture and sports administration, 20 top-scientists experts in the problem under study. Results: the "complex of arrangements that are supposed to involve children and yourth to extracurricular education institutions " was developed the main problem during the pedagogical experiments. Conclusions: the complex introduction and the decision of its main objectives was to increase financial profit by 33% (UK «Ayumi», the number of involved students increas by 24% in the UK «Ayumi» and by 2.2% for the initial training of "SC Youth School in Aquatics von Yana Klochkova.” The effectiveness of implementing measures confirm the results of expert evaluation scholars and practicians in the physical culture and sport spher. Experts from both groups appreciated the level of expected efficiency (4.6 and 5.0 points out of 5 points.

  13. A cross-cultural comparison of sleep duration between U.S. and Australian adolescents: the effect of school start time, parent-set bedtimes and extracurricular load

    NARCIS (Netherlands)

    M.A. Short; M. Gradisar; L.C. Lack; H.R. Wright; J.F. Dewald; A.R. Wolfson; M.A. Carskadon

    2013-01-01

    Study Objective. To test whether sleep duration on school nights differs between adolescents in Australia and the United States and, if so, whether this difference is explained by cultural differences in school start time, parental involvement in setting bedtimes, and extracurricular commitments. Pa

  14. A cross-cultural comparison of sleep duration between U.S. and Australian adolescents: the effect of school start time, parent-set bedtimes and extracurricular load

    NARCIS (Netherlands)

    Short, M.A.; Gradisar, M.; Lack, L.C.; Wright, H.R.; Dewald, J.F.; Wolfson, A.R.; Carskadon, M.A.

    2013-01-01

    Study Objective. To test whether sleep duration on school nights differs between adolescents in Australia and the United States and, if so, whether this difference is explained by cultural differences in school start time, parental involvement in setting bedtimes, and extracurricular commitments.

  15. Importância dos cursos extracurriculares na formação profissional dos acadêmicos do Curso Técnico em Agropecuária - The importance of extracurricular courses in professional training of students of the Agriculture Technical Course

    Directory of Open Access Journals (Sweden)

    Karina Gutierre

    2015-10-01

    Full Text Available Resumo Cursos extracurriculares são uma oportunidade de praticar e aprender assuntos muitas vezes não abordados no decorrer de um curso. Este trabalho objetivou verificar a percepção dos discentes do início e final do Curso Técnico em Agropecuária do Colégio Politécnico da UFSM em relação aos cursos extracurriculares. Por meio de questionário foi observado que os estudantes consideram os cursos extracurriculares importantes para a formação profissional. Também foi observado que grande parte dos estudantes já participou de atividades semelhantes. Os resultados obtidos demonstram a preocupação dos alunos com o futuro profissional e também o papel fundamental de agências financiadoras de atividades na formação qualificada dos estudantes. Palavras-chave: aprendizagem, práticas, currículo, Pronatec. THE IMPORTANCE OF EXTRACURRICULAR COURSES IN PROFESSIONAL TRAINING OF STUDENTS OF THE AGRICULTURE TECHNICAL COURSE Abstract Extracurricular courses are an opportunity to practice and learn subjects not often addressed in the curriculum. This study aimed to determine the perceptions of students from the beginning and end of the Agriculture Technical Course of Colégio Politécnico of UFSM regarding extracurricular courses. Through questionnaire was observed that students from both semesters consider extracurricular courses an important methodology for the training. In addition was observed that most students have already participated in similar activities. These results indicated interest and the preoccupation of the students with the professional future and the fundamental role of financial agencies in the training of qualified students. Key-words: learning, practices, curriculum, Pronatec.

  16. 大学生课余体育活动与身体素质变化调查与分析--以三江学院为例%Investigation and Analysis of Extracurricular Sports Activities and the Change of Students’ Physical Fitness---Taking Sanjiang University as an Example

    Institute of Scientific and Technical Information of China (English)

    崔楠; 王振亚

    2014-01-01

    采用问卷调查法、逻辑分析法等研究方法,以三江学院学生为研究样本,对大学生课余体育活动与身体素质变化进行了调查与分析。结果表明:大学生普遍认为身体素质有下降,又以二四年级为明显,身体素质下降的主要原因各年级各有侧重,运动缺乏是重要原因之一;课余体育活动项目选择中小球类运动最受欢迎;大部分学生对体育课及参加体育社团对课余体育活动有促进作用持肯定态度。%Through using the method of questionnaire and logical analysis, taking students from Sanjiang University as an example , this paper makes analysis on extracurricular sports activities and the change of students ’ physical fitness.The result shows that students ’ physical fitness are declining , especially for Grade 2 and 4.The main reasons causing the physical fitness declining are lack of exercise .The small ball games are very popular among students after-class sports activities .Most students have positive attitude on after -class sports activity .

  17. Operationalising active involvement in the EU water framework directive

    DEFF Research Database (Denmark)

    Wright, Stuart Anthony Lewis; Fritsch, Oliver

    2011-01-01

    of actively involving non-state actors, which can be summarised as increasing the effectiveness of policy and improving its implementation. Criticising the emerging economic decision-making approach, we argue that economic analyses could result in a missed opportunity to capitalise on the potential benefits...... to comply with the statutory requirements of the Directive, but should strive for active involvement due to the potential for increasing the effectiveness of the Water Framework Directive and improving its implementation......We identify two key stages in the river basin planning process under the Water Framework Directive: the selection of instruments for a programme of measures to achieve the environmental targets, and disproportionate cost analysis to determine whether selected measures involve high costs. Some EU...

  18. Active-involvement principle in dental health education

    DEFF Research Database (Denmark)

    Schou, L

    1985-01-01

    A basic problem in dental health education (DHE) is that the effect usually disappears shortly after the termination of a program. The purpose of the present study was to obtain long-term effect of a DHE-program by emphasizing the active involvement of the participants. The sample comprised...

  19. Activity Involvement in Aging Women: Career Pattern and Retirement.

    Science.gov (United States)

    Holahan, Carole Kovalic

    Some research has found that women's retirement from the labor force produces significant changes in their lives and requires further investigation. The effects of career pattern and retirement on activity involvement and life satisfaction for women who had been in the work force was investigated. Subjects were members of the Terman Study of the…

  20. A Study on the Sports Injuries in Extracurricular Activities among the Students from Xi'an University of Arts and Science%西安文理学院学生课外体育活动中运动损伤的调查研究

    Institute of Scientific and Technical Information of China (English)

    陈昱

    2011-01-01

    We made a field study of the sports injuries among the students from Xi'an University of Arts and Science in their extracurricular sports activities,identified the sports injury frequency and the body parts vulnerable to injury,analyzed the reasons and put forward some prevention measures.The findings show that the sports injuries among college students occur more frequently in the sports featuring strong antagonism and direct physical contact and the injured ankle and knee are more commonly found.The frequency of sports injury and injured body parts are closely related with the sports events,preparations,physical fitness,the level of sports technique and tactics.In light of the results,it is recommended that students' consciousness of injury prevention should be enhanced in teaching and training.Meanwhile,health care instruction is highly needed.%对西安文理学院大学生在课外体育活动中发生的运动损伤进行调查分析.了解大学生运动损伤的发生率及易受损的部位,分析原因并找出预防措施.结果发现大学生运动损伤多发生于对抗性强、有直接身体接触的运动项目,损伤部位以踝部、膝部多见.运动损伤的发生率、发生部位等与运动项目、准备活动、身体素质、技战术水平等相关因素关系密切,在平时的体育教学和训练中必须强化学生预防损伤的意识并加强保健指导.

  1. The Arrangement of Students' Extracurricular Piano Practice Process with the Asynchronous Distance Piano Teaching Method

    Science.gov (United States)

    Karahan, Ahmet Suat

    2015-01-01

    That the students do their extracurricular piano practices in the direction of the teacher's warnings is a key factor in achieving success in the teaching-learning process. However, the teachers cannot adequately control the students' extracurricular practices in the process of traditional piano education. Under the influence of this lack of…

  2. The Arrangement of Students' Extracurricular Piano Practice Process with the Asynchronous Distance Piano Teaching Method

    Science.gov (United States)

    Karahan, Ahmet Suat

    2015-01-01

    That the students do their extracurricular piano practices in the direction of the teacher's warnings is a key factor in achieving success in the teaching-learning process. However, the teachers cannot adequately control the students' extracurricular practices in the process of traditional piano education. Under the influence of this lack of…

  3. Extra-Curricular Social Studies in an Open Air History Museum

    Science.gov (United States)

    Morris, Ronald Vaughan

    2008-01-01

    This article discusses extra-curricular social studies in an Open Air History Museum. Open Air History Museum, Conner Prairie Interpretive Park in Fishers, Indiana, is a cultural institution that encourages and supports talented students as they participate in an extra-curricular program. Ten-to sixteen-year-old youths "apply for jobs"…

  4. Investigation and Survey on University Students' Extracurricular Physical Exercise Awareness and Behavior%大学生课外体育锻炼意识与行为的调查分析

    Institute of Scientific and Technical Information of China (English)

    王健

    2011-01-01

    By using questionnaire, literature and mathematic statistics, investigation and survey on undergraduates' extracurricular physical exercise awareness and behavior are carried out, the characteristics and regularity of university student extracurricular physical exercise activities are discussed which provides strategies for cultivating students' interest in sports and lifelong sports consciousness, thus improving extracurricular activity effect and promoting the development of college physical education.%采用问卷调查法、文献资料法和数据统计法,对地方院校大学生参与课外体育活动现状进行调查,探讨大学生课外体育锻炼活动的特点和规律.为培养大学生体育兴趣和终身体育意识,提高课外活动效果,促进高校体育教育的发展提供对策.

  5. Helping General Physical Educators and Adapted Physical Educators Address the Office Of Civil Rights Dear Colleague Guidance Letter: Part VI--Addressing Professional Preparation for Serving Students with Disabilities in Extracurricular Athletic Programs

    Science.gov (United States)

    Silliman-French, Lisa; French, Ron

    2013-01-01

    One of the major components in the development of quality extracurricular athletic (ECA) programs that involves the infusion of students who have been classified as educationally disabled is the preparation of effective, high-quality physical educators who will assume coaching positions (U.S. Government Accountability Office, 2012). These coaches…

  6. [Psychoeducational intervention in high ability: intellectual functioning and extracurricular enrichment].

    Science.gov (United States)

    Sastre-Riba, Sylvia

    2014-02-24

    The 'new paradigm' defines the high intellectual ability as a potential that should crystallize progressively throughout development. Its main feature is a high intellectual initial multidimensional potential, which is transformed so that, being a person with high intellectual ability is the result of a developmental process from a neurobiological substrate and the incidence of variables (psychosocial and education) which determines its manifestation more or less stable and optimal to excellence. It is interesting to know the effectiveness of psychoeducational intervention of the extracurricular enrichment programs and their effects on the expression of differential functioning and the optimization of the management of cognitive resources that lead to excellence. An extracurricular enrichment program is described and evaluated through: 1) the stability of the intellectual measures; 2) the satisfaction level of participants and families. Participants are 58 high ability students on the enrichment program and 25 parents. Intellectual profiles are obtained on T1-T2 and calculated their stability by regression analysis, the CSA and CSA-P questionnaires were applied in order to know the participants and families' satisfaction measure. Results show the basic stability of intellectual profiles with five cases of instability among the 58 profiles obtained, and a high satisfaction with the results obtained in the domain of cognitive and personal management among the participants.

  7. Extracurricular enrichment workshops for high ability students

    Directory of Open Access Journals (Sweden)

    Ángela Rojo

    2010-04-01

    Full Text Available The aim of this article is to analyze and explain attention to diversity measures for high ability (gifted and talented students. The model, developed in the Spanish region of Murcia, is based on cognitive psychology and aims to encourage thinking skills. The program is developed as a curriculum extension and the interests, motivations and abilities of children have been considered once these were identified. The article offers a theoretical approach, a set of objectives, and some of the activities that have been done with students.

  8. Active stream segregation specifically involves the left human auditory cortex.

    Science.gov (United States)

    Deike, Susann; Scheich, Henning; Brechmann, André

    2010-06-14

    An important aspect of auditory scene analysis is the sequential grouping of similar sounds into one "auditory stream" while keeping competing streams separate. In the present low-noise fMRI study we presented sequences of alternating high-pitch (A) and low-pitch (B) complex harmonic tones using acoustic parameters that allow the perception of either two separate streams or one alternating stream. However, the subjects were instructed to actively and continuously segregate the A from the B stream. This was controlled by the additional instruction to listen for rare level deviants only in the low-pitch stream. Compared to the control condition in which only one non-separable stream was presented the active segregation of the A from the B stream led to a selective increase of activation in the left auditory cortex (AC). Together with a similar finding from a previous study using a different acoustic cue for streaming, namely timbre, this suggests that the left auditory cortex plays a dominant role in active sequential stream segregation. However, we found cue differences within the left AC: Whereas in the posterior areas, including the planum temporale, activation increased for both acoustic cues, the anterior areas, including Heschl's gyrus, are only involved in stream segregation based on pitch.

  9. Las características laborales del profesorado de las actividades físico-deportivas extraescolares en función de la edad y el sexo, dentro de la Comunidad de Madrid. (The employment characteristics of teachers of extracurricular sport activities according to their age and gender in the Community of Madrid.

    Directory of Open Access Journals (Sweden)

    Jana María Gallardo-Pérez

    2013-01-01

    Full Text Available ResumenEn relación a la población de las personas que desarrollan la función de docencia de las actividades físico-deportivas extraescolares en la Comunidad de Madrid, los objetivos del presente estudio pretenden describir y analizar la relación entre los tipos de contratos, los ingresos y las variables sociodemográficas. Esta investigación sigue una metodología cuantitativa, de corte transversal y descriptiva en una muestra de 350 personas. El muestro ha sido aleatorio por conglomerados aplicándose una entrevista estandarizada por medio de cuestionario. Los resultados del estudio muestran que existe relación significativa entre el tipo de contrato y la edad [x2 (6= 17.462; p= .008; Φ=.616], la experiencia profesional y el sexo [x2 (36 = 79.047; p=.000; Φ= .475], los ingresos con el tipo de centro educativo donde se realiza la actividad [x2 (14 = 185.922; p=.000; Φ=.745] y el sexo [x2 (7= 15.943; p=.026; Φ=.218]. Las conclusión final del estudio indica que la situación profesional de las personas que trabajan en este ámbito es bastante inestable y precaria.AbstractIn regard to the group of people carrying out the function of teaching extracurricular sport activities in Comunidad de Madrid, the aims of this study are to describe and analyse the relationship among contract, salary and demographic variables. The present study used a quantitative method. A cross-sectional study was performed by means of face-to-face interviews to a random sample of 350 teachers that completed a brief questionnaire. The results show a significant relationship between type contract and age [x2 (6= 17.462; p= .008; Φ=.616], professional experience and gender [x2 (36 = 79.047; p=.000; Φ= .475], salary with type of school [x2 (14 = 185.922; p=.000; Φ=.745] and gender [x2 (7= 15.943; p=.026; Φ=.218]. The general conclusión suggests that the professional situation of the people who work in this area is quite unstable and precarious

  10. Thinking about the Value of Combining Regional Cultural Characteristics in Local Colleges Carrying out College Students' Extracurricular Academic Science and Technology and Campus Cultural Activities--Take Wuyi University as an example%基于地方高校在开展青年大学生课外学术科技及校园文化活动中与区域特色文化相结合的价值思考--以五邑大学为例

    Institute of Scientific and Technical Information of China (English)

    何小勋

    2016-01-01

    Living in China's first overseas only a college of Wuyi University, combination of Wuyi Culture of the humani-ties connotation and young college academic science and technology and campus cultural activities, has a very important theoretical and practical significance. Overseas Chinese cultural theory, cultural cohesion and combine teaching function of young college academic science and technology and campus cultural activities has an important guiding significance, Wuyi Culture on the starting point to realize the value of College Students' extracurricular academic science and technology and campus culture activities.%身处中国第一侨乡的唯一一所本科高校的五邑大学来说,将五邑侨乡文化的人文内涵与高校青年学术科技及校园文化活动相结合,具有非常重要的理论和现实意义。侨乡文化的人本理论、文化凝聚力及寓教功能对高校青年学术科技及校园文化活动具有重要的指导意义,是五邑侨乡文化对高校大学生课外学术科技及校园文化活动价值实现的切入点。

  11. 川渝地区大学生参与课余体育锻炼的调查与研究%On Investigation and Analysis of College Students Taking Part in Extracurricular Physical Activities

    Institute of Scientific and Technical Information of China (English)

    吴琼先

    2015-01-01

    采用文献资料法、问卷调查法、访谈法和数理统计法,对川渝地区大学生课余体育锻炼现状进行了分析,结果表明:有85.8%的大学生表示喜欢和喜欢参加体育锻炼,但体育态度和体育行为之间不完全一致;男生喜欢体育锻炼的人数比例比女生高;大学生参与体育锻炼的目的多样化,注重体育功能的全面开发;男生喜欢有激烈对抗、趣味性较强的运动,女生喜欢没有直接身体对抗,较为舒缓的运动;大学生锻炼的时间主要集中在傍晚和晚上,高峰时期,绝大部分高校体育场馆设施不够充足;大学生体育人口约为45%,高于全国平均水平,部分学生体育锻炼的方法欠合理;参加体育锻炼的大学生中,以“学校体育场馆”为主要锻炼场所;体育场馆设施不足和缺乏锻炼伙伴是影响大学生参加体育锻炼的主要障碍;大学生的体育消费意识较好;大学生对毕业后继续参加体育锻炼表现出忧虑情绪.%By means of literature consultation ,questionnaire ,interview and mathematical statistics ,inves‐tigation and analysis have been done to discuss the situation on college students taking part in extracurricu‐lar physical activities .T he result show s that 85 .8% of all the students like sports and take part in physical activities ,14 .2% don't like and don't join ,the attitude and behavior to sports are not always same;the proportion of boys take part in exercise is more than girls ;the aim ,that college students take part in exer‐cise ,which emphasize the whole explore about physics function ,is diversification ;boys like those items with intense resistance and strong interest ,but the girls like the sports without intense resistance of body , soft and slow ;the period when students take part in physical exercise is focus on nightfall and evening , during the peak w hen college join exercise ,most of college stadiums have

  12. The Mode of Extracurricular Physical Education in Private Higher Vocational Colleges%民办高职院校课外体育模式的探析

    Institute of Scientific and Technical Information of China (English)

    闫景宏

    2012-01-01

    本文着重调查分析了陕西省高职院校如何组织大学生参与课外体育的模式,旨在促进高职院校在校学生参加课外体育活动,培养学生体育锻炼的意识,提高学生体质,培养学生终身体育意识.%This article focuses on how to organize students participating in extracurricular sports in Shaanxi higher vocational colleges to promote the students participating in extracurricular sports activities, develop students' consciousness of physical exercises, improve students' physical fitness, and train students' life-long sports consciousness.

  13. 四川省中小学生体育活动与课余活动调查分析--以成都、自贡、达州为例%Analysis of Sports Activities and Extracurricular Activity Survey On Primary and Secondary School Students in Sichuan Province--Taking Chengdu, Zigong and Dazhou as an example

    Institute of Scientific and Technical Information of China (English)

    张艺宏; 孙君志; 李宁; 赵娜; 杜会娟

    2015-01-01

    study based on the work of revising about National Student Physical Health Standard organized by National Department of Education. Result:(1)78.5%of the students like (like very much) the PE (physical education), and more boys than girls, and more primary students than secondary. (2)The top five favorite’s sports programs were, in order, BVF (basketball, volleyball, and football), TB (table tennis, badminton), swimming, and sport game, roller-skating. 60.6%of the students like (like very much) the long distance running. (3)76.4% of the students spend 30minutes to 2 hours on exercise every day. 37.8%of the students spend over one hour, and 18.9%of the students spend less than half an hour for exercise every day. (4) 81%of the students have 2-3 PE classes a week. 65%of the students have a little bit tired in PE. 59%of the students take setting-up exercises only once a day. (5)54.7%of the students have more than 8 hours' sleep and 26.7%have less than 7 hours' sleep. (6)36.1%of the students spend 30minutes to one hour on homework every day and 38.7%of students spend more than one hour on homework. 18.4%of students spend on time on watching TV, playing video game and computer. But 50%of students spend less 30 minutes on that, 31.6%of students spend more than 30 minutes on that. Conclusion: (1)The most of primary and secondary school students like PE in Sichuan province, and their favorites sport are basketball, volleyball, football, table tennis, badminton, swimming, sport game and roller-skating.(2)The most of primary and secondary school students have 2-3 PE classes a week and take setting-up exercises once a day. Less than 40 percent of students spend more than one hour on exercise every day. ( 3 ) The outstanding issues in extracurricular activities are lack of sleep, excessive homework and lack of necessary leisure activities.

  14. Should Ecosystem Management Involve Active Control of Species Abundances?

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    Timothy E. Essington

    2005-12-01

    Full Text Available We review four case studies in which there is a risk of extinction or severe reduction in highly valued species if we ignore either, or both, of two ecosystem control options. “Symptomatic control” implies direct control of extinction risk through direct harvesting or culling of competitors and predators. “Systemic control” implies treating the causes of the problem that led to an unnaturally high abundance in the first place. We demonstrate, with a discussion of historically observed population trends, how surprising trophic interactions can emerge as a result of alterations to a system. Simulation models were developed for two of the case studies as aids to adaptive policy design, to expose possible abundance changes caused by trophic interactions and to highlight key uncertainties about possible responses to ecosystem management policies involving active intervention to control abundances. With reasonable parameter values, these models predict a wide range of possible responses given available data, but do indicate a good chance that active control would reverse declines and reverse extinction risks. We find that controlling seal (Phoca vitulina populations in the Georgia Strait increases juvenile survival rates of commercial salmon (Oncorhynchus spp. species, but that commensurate increases in hake populations from decreased seal predation could be a compensatory source of predation on juvenile salmon. We also show that wolf (Canis lupus control and moose (Alces alces harvest bring about a recovery in caribou (Rangifer tarandus caribou populations, where simple habitat protection policies fail to recover caribou before wolf predation causes severe declines. The results help address a common problem in disturbed ecosystems, where controlling extinction risks can mean choosing between active control of species abundance or establishing policies of protection, and allowing threatened species to recover naturally.

  15. Better Education at Ishik University Preparatory School with Extracurricular Activities

    Science.gov (United States)

    Yildiz, Yunus

    2015-01-01

    It cannot be said that education today in institutions is better than the previous century. Because in the past, students' minds were not as full of time-consuming things like spending enormous time in front of a computer or a television as today. Subsequently, teachers used to concentrate their job well and students used to focus on the study…

  16. The Contribution of Ideational Behavior to Creative Extracurricular Activities

    Science.gov (United States)

    Paek, Sue Hyeon; Park, Hyeri; Runco, Mark A.; Choe, Ho-Seong

    2016-01-01

    The prediction of creative performance from ideation has shown promise, but questions remain. In this study, the relationship of ideational behavior and the creative performance of elementary school children (N = 255) was examined across 6 domains of creative performance: science, mathematics, technology, fine arts, music, and writing.…

  17. Professional Identities of Vocational High School Students and Extracurricular Activities

    Science.gov (United States)

    Altan, Bilge Aslan; Altintas, Havva Ozge

    2017-01-01

    Vocational high schools are one of the controversial topics, and also the hardly touched fields in educational field. Students' profiles of vocational schools, their visions, and professional identity developments are not frequently reflected in the literature. Therefore, the main aim of the study is to research whether vocational high school…

  18. Consumer involvement in research projects: the activities of research funders.

    Science.gov (United States)

    O'Donnell, Máire; Entwistle, Vikki

    2004-08-01

    This paper reports findings from a postal questionnaire survey and in-depth interviews with UK funders of health-related research that explored whether, why and how they promote consumer involvement in research projects. Many UK funders of health-related research are adopting a policy of promoting consumer involvement in research projects. Telephone interviews revealed they have several reasons for doing so, and that they vary in the ways they encourage and support researchers to involve consumers. For some, descriptions of consumer involvement in a research proposal are important for project funding decisions. They recognized a need for flexibility when assessing consumer involvement in different contexts. We suggest that funders should continue to work to clarify what they consider to be the parameters of acceptability in terms of consumer involvement and ensure that 'flexible' criteria are fairly applied. Researchers should be aware of particular funders' views when applying for project funding.

  19. SIGNIFICANT INFLUENCES OF VIOLIN EXTRACURRICULAR ACHIEVEMENT TO EMOTIONAL INTELLIGENCE

    Directory of Open Access Journals (Sweden)

    Nafik

    2014-06-01

    Full Text Available This research aims to find out (1 whether there is an influence between student’s achievements of learning violin toward their emotional intelligence, (2 whether there is a correlation between student’s achievement of learning violin and their emotional intelligence, and (3 how much contribution of student’s achievement of learning violin to their emotional intelligence. It is a qualitative research which is defined as a research method based on positivism philosophy which is used to study particular sample and population. The sample and population are drawn randomly using research instruments to collect data, and the data are analyzed statistically. This aims to examine the hypothesis defined. The finding shows that there is a significant influence between student’s achievement of learning violin and their emotional intelligence about 76.1%, while the rest of it 23.9% is influenced by other factors which are not studied in this research. It proves that learning violin influences student’s emotional intelligence very much and emotional intelligence is influential in increasing student’s achievement. From the data, it shows that most of the students participating in violin extracurricular are able to increase their learning achievement.

  20. 101 Activities for Building More Effective School-Community Involvement.

    Science.gov (United States)

    Rich, Dorothy; Mattox, Beverly

    This booklet contains a collection of more than 100 activities designed to promote school-home and school-community relations. Activities are organized into seven categories: (1) communicating word from home to school, (2) people to people, (3) educational events, (4) volunteers--hands on in the classroom, (5) utilizing community resources, (6)…

  1. A contextual perspective on talented female participants and their development in extracurricular STEM programs.

    Science.gov (United States)

    Stoeger, Heidrun; Schirner, Sigrun; Laemmle, Lena; Obergriesser, Stefanie; Heilemann, Michael; Ziegler, Albert

    2016-08-01

    We advocate a more contextual perspective in giftedness research. In our view, doing so opens up three particularly interesting research areas, which we refer to as the participation issue, the effectiveness issue, and the interaction issue. To illustrate their utility, we examined characteristics of females participating in German high achiever-track secondary education who had applied for participation in a 1-year extracurricular e-mentoring program in science, technology, engineering, and mathematics (STEM) (n = 1237). Their characteristics were compared with male and female random-sample control groups. We assessed the effectiveness of the mentoring program by comparing the developmental trajectories of program participants with those of three control groups: applicants who were randomly chosen for later participation (waiting-list control group) and a female and a male control group. Finally, we examined whether differences in program effectiveness could be partially explained by characteristics of the interaction with the domain. Program applicants possessed more advantageous individual characteristics but, unexpectedly, less advantageous home and school environments than female and male members of the control groups. Program participation affected positive changes in certainty about career goals (independent of STEM) and in the number of STEM activities. The amount of STEM communication partially explained differences in program effectiveness. © 2016 New York Academy of Sciences.

  2. Practical recommendations for the implementation of health technologies to enhance physical fitness of students in extracurricular classes during non-traditional gymnastics

    Directory of Open Access Journals (Sweden)

    Fomenko E.V.

    2014-04-01

    Full Text Available Purpose : to develop practical recommendations for extracurricular classes nontraditional kinds of gymnastics to improve the organization of physical education teachers in schools. Material : in the experiment involved 358 students. Analyzed the available literature data. Results : a comparative analysis of physical fitness of students and practical recommendations for the non-traditional occupations gymnastics. Been a significant interest in physical education classes. Found that the main ways of improving physical education students may be the formation of the need for strengthening health facilities fitness aerobics, shaping, pilates. Conclusions : highlights the need to structure the problems they need and develop appropriate solutions.

  3. Particle emissions from laboratory activities involving carbon nanotubes

    Science.gov (United States)

    Lo, Li-Ming; Tsai, Candace S.-J.; Heitbrink, William A.; Dunn, Kevin H.; Topmiller, Jennifer; Ellenbecker, Michael

    2017-08-01

    This site study was conducted in a chemical laboratory to evaluate nanomaterial emissions from 20-30-nm-diameter bundles of single-walled carbon nanotubes (CNTs) during product development activities. Direct-reading instruments were used to monitor the tasks in real time, and airborne particles were collected using various methods to characterize released nanomaterials using electron microscopy and elemental carbon (EC) analyses. CNT clusters and a few high-aspect-ratio particles were identified as being released from some activities. The EC concentration (0.87 μg/m3) at the source of probe sonication was found to be higher than other activities including weighing, mixing, centrifugation, coating, and cutting. Various sampling methods all indicated different levels of CNTs from the activities; however, the sonication process was found to release the highest amounts of CNTs. It can be cautiously concluded that the task of probe sonication possibly released nanomaterials into the laboratory and posed a risk of surface contamination. Based on these results, the sonication of CNT suspension should be covered or conducted inside a ventilated enclosure with proper filtration or a glovebox to minimize the potential of exposure.

  4. 45 CFR 1177.4 - Claims involving criminal activity or misconduct.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Claims involving criminal activity or misconduct. 1177.4 Section 1177.4 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL... Claims involving criminal activity or misconduct. (a) A debtor whose indebtedness involves...

  5. Dopamine is involved in food-anticipatory activity in mice.

    Science.gov (United States)

    Liu, Yuan-Yuan; Liu, Tian-Ya; Qu, Wei-Min; Hong, Zong-Yuan; Urade, Yoshihiro; Huang, Zhi-Li

    2012-10-01

    When food is available during a restricted and predictable time of the day, mammals exhibit food-anticipatory activity (FAA), an increase in locomotor activity preceding the presentation of food. Although many studies have attempted to locate the food-entrainable circadian oscillator in the central nervous system, the pathways that mediate food entrainment are a matter of controversy. The present study was designed to determine the role of dopaminergic and histaminergic systems on FAA. Mice were given access to food for 2 h (ZT12-ZT14), and FAA was defined as the locomotor activity that occurred 2 h before the availability of food. Dopamine D(1) receptor (R), D(2)R, and histamine H(1)R-specific antagonists were used to clarify the role of dopamine and histamine receptors in FAA induced by food restriction (FR). FAA was monitored by infrared locomotor activity sensors. Mice were sacrificed at ZT12 on the 14th day of FR, and monoamine concentrations were determined by high-performance liquid chromatography coupled to electrochemical detection (HPLC-ECD). The results showed that pretreatment with the D(1)R antagonist SCH23390 at 1, 3, or 10 µg/kg significantly reduced FAA by 19% (p antagonist raclopride at 22, 67, or 200 µg/kg significantly reduced FAA by 16% (p dopamine and its metabolites in the striatum and midbrain were significantly increased during FAA, even with the pretreatment of D(1)R and D(2)R antagonists. However, pretreatment with pyrilamine at 2.5, 5, or 10 mg/kg did not significantly reduce FAA, although it reduced the locomotor activity during the dark period in ad libitum mice. These results strongly indicate that the dopaminergic system plays an essential role in the FAA in mice.

  6. Proposals for risk management in environments with activities involving nanomaterials

    Directory of Open Access Journals (Sweden)

    Luís Renato Balbão Andrade

    2013-11-01

    Full Text Available The handling of nanomaterials presents enormous challenges for risk management in research and production of new materials. However, data on the impacts of these new materials on human health and the environment need to be expanded. Several efforts have been made to mitigate the hardships and offer guidelines for the management of risks associated with nanomaterials. This article aims to provide a broad and comparing view of the main proposals in the literature. The methodology was systematic analysis encompassing 17 proposed risk management with nanomaterials. The results indicate that, although there is no consensus on the metrics used to characterize the risks of na-nomaterials, the adoption of the Precautionary Principle, the control banding approach and stakeholder involvement stands out among the documents analyzed.

  7. Involvement of hypothalamic AMP-activated protein kinase in leptin-induced sympathetic nerve activation.

    Directory of Open Access Journals (Sweden)

    Mamoru Tanida

    Full Text Available In mammals, leptin released from the white adipose tissue acts on the central nervous system to control feeding behavior, cardiovascular function, and energy metabolism. Central leptin activates sympathetic nerves that innervate the kidney, adipose tissue, and some abdominal organs in rats. AMP-activated protein kinase (AMPK is essential in the intracellular signaling pathway involving the activation of leptin receptors (ObRb. We investigated the potential of AMPKα2 in the sympathetic effects of leptin using in vivo siRNA injection to knockdown AMPKα2 in rats, to produce reduced hypothalamic AMPKα2 expression. Leptin effects on body weight, food intake, and blood FFA levels were eliminated in AMPKα2 siRNA-treated rats. Leptin-evoked enhancements of the sympathetic nerve outflows to the kidney, brown and white adipose tissues were attenuated in AMPKα2 siRNA-treated rats. To check whether AMPKα2 was specific to sympathetic changes induced by leptin, we examined the effects of injecting MT-II, a melanocortin-3 and -4 receptor agonist, on the sympathetic nerve outflows to the kidney and adipose tissue. MT-II-induced sympatho-excitation in the kidney was unchanged in AMPKα2 siRNA-treated rats. However, responses of neural activities involving adipose tissue to MT-II were attenuated in AMPKα2 siRNA-treated rats. These results suggest that hypothalamic AMPKα2 is involved not only in appetite and body weight regulation but also in the regulation of sympathetic nerve discharges to the kidney and adipose tissue. Thus, AMPK might function not only as an energy sensor, but as a key molecule in the cardiovascular, thermogenic, and lipolytic effects of leptin through the sympathetic nervous system.

  8. Involvement of hypothalamic AMP-activated protein kinase in leptin-induced sympathetic nerve activation.

    Science.gov (United States)

    Tanida, Mamoru; Yamamoto, Naoki; Shibamoto, Toshishige; Rahmouni, Kamal

    2013-01-01

    In mammals, leptin released from the white adipose tissue acts on the central nervous system to control feeding behavior, cardiovascular function, and energy metabolism. Central leptin activates sympathetic nerves that innervate the kidney, adipose tissue, and some abdominal organs in rats. AMP-activated protein kinase (AMPK) is essential in the intracellular signaling pathway involving the activation of leptin receptors (ObRb). We investigated the potential of AMPKα2 in the sympathetic effects of leptin using in vivo siRNA injection to knockdown AMPKα2 in rats, to produce reduced hypothalamic AMPKα2 expression. Leptin effects on body weight, food intake, and blood FFA levels were eliminated in AMPKα2 siRNA-treated rats. Leptin-evoked enhancements of the sympathetic nerve outflows to the kidney, brown and white adipose tissues were attenuated in AMPKα2 siRNA-treated rats. To check whether AMPKα2 was specific to sympathetic changes induced by leptin, we examined the effects of injecting MT-II, a melanocortin-3 and -4 receptor agonist, on the sympathetic nerve outflows to the kidney and adipose tissue. MT-II-induced sympatho-excitation in the kidney was unchanged in AMPKα2 siRNA-treated rats. However, responses of neural activities involving adipose tissue to MT-II were attenuated in AMPKα2 siRNA-treated rats. These results suggest that hypothalamic AMPKα2 is involved not only in appetite and body weight regulation but also in the regulation of sympathetic nerve discharges to the kidney and adipose tissue. Thus, AMPK might function not only as an energy sensor, but as a key molecule in the cardiovascular, thermogenic, and lipolytic effects of leptin through the sympathetic nervous system.

  9. WRKY Transcription Factors Involved in Activation of SA Biosynthesis Genes

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    Bol John F

    2011-05-01

    Full Text Available Abstract Background Increased defense against a variety of pathogens in plants is achieved through activation of a mechanism known as systemic acquired resistance (SAR. The broad-spectrum resistance brought about by SAR is mediated through salicylic acid (SA. An important step in SA biosynthesis in Arabidopsis is the conversion of chorismate to isochorismate through the action of isochorismate synthase, encoded by the ICS1 gene. Also AVRPPHB SUSCEPTIBLE 3 (PBS3 plays an important role in SA metabolism, as pbs3 mutants accumulate drastically reduced levels of SA-glucoside, a putative storage form of SA. Bioinformatics analysis previously performed by us identified WRKY28 and WRKY46 as possible regulators of ICS1 and PBS3. Results Expression studies with ICS1 promoter::β-glucuronidase (GUS genes in Arabidopsis thaliana protoplasts cotransfected with 35S::WRKY28 showed that over expression of WRKY28 resulted in a strong increase in GUS expression. Moreover, qRT-PCR analyses indicated that the endogenous ICS1 and PBS3 genes were highly expressed in protoplasts overexpressing WRKY28 or WRKY46, respectively. Electrophoretic mobility shift assays indentified potential WRKY28 binding sites in the ICS1 promoter, positioned -445 and -460 base pairs upstream of the transcription start site. Mutation of these sites in protoplast transactivation assays showed that these binding sites are functionally important for activation of the ICS1 promoter. Chromatin immunoprecipitation assays with haemagglutinin-epitope-tagged WRKY28 showed that the region of the ICS1 promoter containing the binding sites at -445 and -460 was highly enriched in the immunoprecipitated DNA. Conclusions The results obtained here confirm results from our multiple microarray co-expression analyses indicating that WRKY28 and WRKY46 are transcriptional activators of ICS1 and PBS3, respectively, and support this in silico screening as a powerful tool for identifying new components of stress

  10. Hemolysis-induced lethality involves inflammasome activation by heme.

    Science.gov (United States)

    Dutra, Fabianno F; Alves, Letícia S; Rodrigues, Danielle; Fernandez, Patricia L; de Oliveira, Rosane B; Golenbock, Douglas T; Zamboni, Dario S; Bozza, Marcelo T

    2014-09-30

    The increase of extracellular heme is a hallmark of hemolysis or extensive cell damage. Heme has prooxidant, cytotoxic, and inflammatory effects, playing a central role in the pathogenesis of malaria, sepsis, and sickle cell disease. However, the mechanisms by which heme is sensed by innate immune cells contributing to these diseases are not fully characterized. We found that heme, but not porphyrins without iron, activated LPS-primed macrophages promoting the processing of IL-1β dependent on nucleotide-binding domain and leucine rich repeat containing family, pyrin domain containing 3 (NLRP3). The activation of NLRP3 by heme required spleen tyrosine kinase, NADPH oxidase-2, mitochondrial reactive oxygen species, and K(+) efflux, whereas it was independent of heme internalization, lysosomal damage, ATP release, the purinergic receptor P2X7, and cell death. Importantly, our results indicated the participation of macrophages, NLRP3 inflammasome components, and IL-1R in the lethality caused by sterile hemolysis. Thus, understanding the molecular pathways affected by heme in innate immune cells might prove useful to identify new therapeutic targets for diseases that have heme release.

  11. FAMILY INVOLVEMENT ACTIVITIES AT PRESCHOOL EDUCATIONAL INSTITUTIONS: CASE OF DENIZLI PROVINCE

    Directory of Open Access Journals (Sweden)

    Hülya GÜLAY OGELMAN

    2014-06-01

    Full Text Available The purpose of this study is to investigate family involvement activities at preschool educational institutions in province of Denizli. 25 female teachers working at preschool educational institutions under Ministry of National Education in city centre of Denizli were included in the study. The results revealed that while a great majority of 32 family involvement activities included in the questionnaire (parents' corner, posters, education board, communication board, brochures, booklets, periodicals-newspapers, newsletters, correspondences, photographs, phone/ internet calls, video/tape records, conferences, home visits, article box, request box, parents' libraries, family involvement to non-classroom activities, diaries, family involvement into management and decision-making process, group meeting weren’t performed, 11 activities were implemented. While 4 of 11 activities were performed by all teachers, there were teachers who didn't perform 7 family involvement activities (parents' meetings, individual interview, development reports, collective files, involvement to classroom activities, invitation of parents to the school in special occasions, activities held at home. Considering the reasons why twenty two family involvement activities were not performed; lack of space, time and possibilities was the most frequent reason. Home visits took place on the top among family involvement activities the teachers had difficulties at most. The individual interviews were among activities conducted by the teachers in the easiest/ comfortable way.

  12. Writing as Embodied, College Football Plays as Embodied: Extracurricular Multimodal Composing

    Science.gov (United States)

    Rifenburg, J. Michael

    2014-01-01

    Recent explorations position multimodality as a largely curricular practice wherein the body typically is not figured as a potential mode of meaning making. Such a projection not only fails to acknowledge extracurricular uses of such a rhetoric but also fails to acknowledge the role of the body in and especially for composing. In hopes of…

  13. Gender Differences on Attitudes and Participation in an Extracurricular Gymnastics Course among Greek University Students

    Science.gov (United States)

    Milosis, Dimitrios; Papaioannou, Athanasios G.; Siatras, Theophanis A.; Proios, Miltiadis; Proios, Michael

    2015-01-01

    The aims of the study were (a) to test the effectiveness of the theory of planned behavior (TPB) to predict Greek university students' voluntary participation in an extracurricular gymnastics course, and (b) to evaluate gender differences. Two hundred sixty-three (127 female, 136 male) students participated in the study. Students' attitudes,…

  14. Australian Primary Students' Motivation and Learning Intentions for Extra-Curricular Music Programmes

    Science.gov (United States)

    Ng, Clarence

    2017-01-01

    What are the motivational differences between students who intend to continue their learning in instrumental and choral music programmes and those who intend to discontinue? Using an achievement-goal perspective, this study investigated motivation and learning intentions of Australian students who had engaged in these extra-curricular music…

  15. A Review on Extra-Curricular English Course in Replacement of Graduation Certification

    Science.gov (United States)

    Lee, Eunpyo; Kim, Eun-Kyung

    2014-01-01

    This study examines an extra-curricular English course that gives a chance for the low-proficient seniors who have not yet met the graduation qualification to graduate upon completion of 90 hours of English program. The subjects were 14 seniors who failed to obtain the minimum requirement scores and had to complete the course in summer of 2014. A…

  16. Gender Differences on Attitudes and Participation in an Extracurricular Gymnastics Course among Greek University Students

    Science.gov (United States)

    Milosis, Dimitrios; Papaioannou, Athanasios G.; Siatras, Theophanis A.; Proios, Miltiadis; Proios, Michael

    2015-01-01

    The aims of the study were (a) to test the effectiveness of the theory of planned behavior (TPB) to predict Greek university students' voluntary participation in an extracurricular gymnastics course, and (b) to evaluate gender differences. Two hundred sixty-three (127 female, 136 male) students participated in the study. Students' attitudes,…

  17. Predicting involvement in prison gang activity: street gang membership, social and psychological factors.

    Science.gov (United States)

    Wood, Jane L; Alleyne, Emma; Mozova, Katarina; James, Mark

    2014-06-01

    The aim of this study was to examine whether street gang membership, psychological factors, and social factors such as preprison experiences could predict young offenders' involvement in prison gang activity. Data were collected via individual interviews with 188 young offenders held in a Young Offenders Institution in the United Kingdom. Results showed that psychological factors such as the value individuals attached to social status, a social dominance orientation, and antiauthority attitudes were important in predicting young offenders' involvement in prison gang activity. Further important predictors included preimprisonment events such as levels of threat, levels of individual delinquency, and levels of involvement in group crime. Longer current sentences also predicted involvement in prison gang activity. However, street gang membership was not an important predictor of involvement in prison gang activity. These findings have implications for identifying prisoners involved in prison gang activity and for considering the role of psychological factors and group processes in gang research.

  18. 5 CFR 1215.24 - Claims involving criminal activity or misconduct.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Claims involving criminal activity or misconduct. 1215.24 Section 1215.24 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES DEBT MANAGEMENT Claims Collection § 1215.24 Claims involving criminal activity or misconduct....

  19. Ego Strength Development of Adolescents Involved in Adult-Sponsored Structured Activities.

    Science.gov (United States)

    Markstrom, Carol A.; Li, Xaioming; Blackshire, Shana L.; Wilfong, Juanita J.

    2005-01-01

    A psychosocial conception of ego strengths is presented in relation to adolescent involvement in adult-sponsored structured youth activities. Five-hundred and seventeen high school students completed measures on their involvement in structured activities and on 8 ego strengths. Gender, age, and SES were controlled in a MANCOVA procedure and it was…

  20. Empathy and Extracurricular Involvement in Emerging Adulthood: Findings from a Longitudinal Study of Undergraduate College Males

    Science.gov (United States)

    Hudson-Flege, Matthew; Thompson, Martie P.

    2017-01-01

    In this study, we analyzed the Interpersonal Reactivity Index Perspective Taking subscale scores for male college students in a 2008-2011 longitudinal study at a large public university in the Southeast. Findings suggest that empathy is amenable to change among college males in the period of emerging adulthood. Through repeated measures analyses…

  1. 微电影在课堂外英语自主学习中的应用%Application of Microfilms in Extra-curricular English Autonomous Learning

    Institute of Scientific and Technical Information of China (English)

    杜秀红

    2015-01-01

    With the cut-down of college English classes and the deepening of the lifelong learning concept,the extracurricular au-tonomous learning is increasingly important. As a new thing in digital era,microfilms are broadcast in via of mobile media and net-works. Its features of“three-micro”,wide citizen involvement and super interactivity correspond with the values of university students. The paper tries to analyze the functions of the microfilm in improving extracurricular autonomous learning capacity of college students from aspects of narrative features and broadcasting characteristics.%随着大学英语学时缩减和终身学习理念深入人心,课堂外自主学习的重要性凸显。作为数字网络时代的新生事物,微电影借助各种移动新媒体和网络平台传播,其三“微”特征、全民参与性和超强互动性等特点符合大学生的价值偏好。从微电影叙事特征和传播特点等方面分析其在提高大学生课堂外自主学习能力的作用。

  2. Factors affecting construction of science discourse in the context of an extracurricular science and technology project

    Science.gov (United States)

    Webb, Horace P.

    Doing and learning science are social activities that require certain language, activities, and values. Both constitute what Gee (2005) calls Discourses. The language of learning science varies with the learning context (Lemke, 2001,1990). Science for All Americans (AAAS, 1990) and Inquiry and the National Science Education Standards (NRC, 2000) endorse inquiry science learning. In the United States, most science learning is teacher-centered; inquiry science learning is rare (NRC, 2000). This study focused on 12 high school students from two suburban high schools, their three faculty mentors, and two engineering mentors during an extracurricular robotics activity with FIRST Robotics Competition (FRC). FRC employed student-centered inquiry focus to teach science principles integrating technology. Research questions were (a) How do science teachers and their students enact Discourses as they teach and learn science? and (b) How does the pedagogical approach of a learning activity facilitate the Discourses that are enacted by students and teachers as they learn and teach science? Using Critical Discourse Analysis (CDA), the study examined participants' language during robotic activities to determine how language used in learning science shaped the learning and vice versa. Data sources included videorecordings of participant language and semi-structured interviews with study participants. Transcribed recordings were coded initially using Gee's (2005) linguistic Building Tasks as a priori codes. CDA was applied to code transcripts, to construct Discourses enacted by the participants, and to determine how context facilitated their enactment. Findings indicated that, for the students, FRC facilitated elements of Science Discourse. Wild About Robotics (W.A.R.) team became, through FRC, part of a community similar to scientists' community that promoted knowledge and sound practices, disseminated information, supported research and development and encouraged interaction of

  3. Parent Involvement in Early Childhood Special Education: Selected Activities for Preschool Teachers.

    Science.gov (United States)

    Carlson, Betty Clark

    The manual is designed to provide special education preschool teachers with a guide for parent involvement activities. The guide presents 32 activities divided into three topical areas: (1) communicating with parents (orientation packet, newsletter, school-home notebook, bulletin board for parents); (2) resource activities (field trips, home…

  4. 提高大学生课外体育锻炼参与度的策略研究%Research on Strategies Improving the Participation of College Students'in Extracurricular Physical Exercise

    Institute of Scientific and Technical Information of China (English)

    安涛

    2015-01-01

    课外体育锻炼是学生参与体育活动的一部分,也是提高大学生身体素质的基础,但各级各类学校落实开展的情况并不乐观,在体育课程开展有限,课外体育锻炼参与度不理想,日益不能满足提高大学生身体素质的情况下,结合课外体育锻炼的目的意义。提出:提高大学生课外锻炼参与度应注意从课外锻炼的可持续发展系统、保障服务系统、动力系统等三个方面入手进行考虑。%Extracurricular physical exercise is a part of students'participation in sports activities as well as the basis of improving their physical quality,but its carrying out in all types of schools at all levels is not optimistic. Faced with limited P.E.classes,weak participation of extracurricular physical exercise and failure to promote physical quality of college students, this paper suggests improving participation of college students 'in extracurricular physical exercise from aspects of the system of sustainable development of extracurricular physical exercise,security service system and motivation system by taking into consideration the goals of extracurricular physical exercise.

  5. Extracurricular scientific production among medical students has increased in the past decade

    DEFF Research Database (Denmark)

    Bech Andersen, Sofie; Østergaard, Lauge; Fosbøl, Philip Loldrup

    2015-01-01

    Introduction: Undergraduate research among medical students is essential in the education of future physicians and scientists. This study aimed to evaluate the scientific yield of extracurricular undergraduate research among medical students. Methods: Medical students at the University of Copenha...... in cardiology (14.1%). Car - diology was also associated with the greatest scientific yield with a median number of 0.8 publications per year after the students concluded their undergraduate research period. Three or more years after concluding their undergraduate research, 32.8% of the students had continued...... with re - search in the context of a PhD programme. c onclus I on: Overall, the number of medical students who engage in extracurricular research follows an increasing trend, and more than two-thirds of these students publish a peer-reviewed paper within three years. Cardiology was the most popular...

  6. 16 CFR 1031.5 - Criteria for Commission involvement in voluntary standards activities.

    Science.gov (United States)

    2010-01-01

    ... Commission involvement in the development of voluntary safety standards for consumer products: (a) The... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Criteria for Commission involvement in voluntary standards activities. 1031.5 Section 1031.5 Commercial Practices CONSUMER PRODUCT...

  7. Autonomous motivation: involvement in physical activity, and perceived sport competence: structural and mediator models.

    Science.gov (United States)

    Bagøien, Tor Egil; Halvari, Hallgeir

    2005-02-01

    Students in upper secondary school (N = 231, M = 16.6 yr., SD = 1.6) were tested on involvement in physical activity, perceived sport competence, using the Perceived Competence Scale of Harter, and motivational regulation on the Self-regulation Questionnaire of Ryan and Connell. Correlations were positive among involvement in physical activity, autonomous motivation, and perceived sport competence. A hypothetical model indicated that autonomous motivation mediates the relation between perceived sport competence and involvement in physical activity. Although LISREL analysis supported this mediation, the best model fit of the data supported a structural model with involvement in physical activity (R2 = .63) to mediate between autonomous motivation and perceived competence (R2 = .47). Results are interpreted and discussed in terms of self-determination theory.

  8. 78 FR 57818 - Commission Participation and Commission Employee Involvement in Voluntary Standards Activities

    Science.gov (United States)

    2013-09-20

    ... From the Federal Register Online via the Government Publishing Office CONSUMER PRODUCT SAFETY COMMISSION 16 CFR Part 1031 Commission Participation and Commission Employee Involvement in Voluntary Standards Activities AGENCY: Consumer Product Safety Commission. ACTION: Notice of proposed...

  9. Ways Discussion and Needs Analysis of Extra--curricular Reading for Higher Vocational College Students%高职学生课余阅读需求分析及途径探讨

    Institute of Scientific and Technical Information of China (English)

    宋小梅

    2012-01-01

    The extra--curricular reading is an important way to acquire knowledge for vocational students. According to analyzing the questionnaire of extra--curricular reading requirements for vocational students, some ways are put forward to improve extra--curricular reading quality for high vocational students to obtain knowledge and get nurtured, which are improving reading level in class teaching, using elective courses to extend reading time, and carrying out campus activities to increase reading.%课余阅读是高职学生获取知识的重要途径,根据调查问卷分析高职学生的课余阅读需求,有针对性地引导高职生的课余阅读,通过在课堂教学中提升阅读水平,利用选修课扩展阅读时间,开展校园活动增加阅读等途径来提高高职学生的课余阅读质量,从而获得知识,得到熏陶。

  10. Ways to Stimulate Students' Interest in Extracurricular Classics Reading%激发学生阅读课外名著兴趣的方法

    Institute of Scientific and Technical Information of China (English)

    周占江

    2016-01-01

    学生的课外阅读面窄而量小,是当前的普遍现象,直接制约了学生语文学习乃至全面发展。培养课外阅读兴趣,是语文教学关键,而落实起来却有许多困难。对此,教师应积极研究培养和激发学生课外阅读兴趣的有效途径和方法,以使学生获得更好发展。%Students' extracurricular reading range is narrow and small, which is the current general phenomenon and directly restricts the students' Chinese study and comprehensive development. To develop extracurricular reading interest, Chinese teaching is the key, but it has many difficulties to implement. As a result, teachers should actively study the effective ways to cultivate and stimulate students' reading interest and methods, in order to make students get a better development.

  11. Health benefits of serious involvement in leisure activities among older Korean adults

    Science.gov (United States)

    Kim, Junhyoung; Yamada, Naoko; Heo, Jinmoo; Han, Areum

    2014-01-01

    The existing literature suggests that serious engagement in leisure activities leads to happiness, life satisfaction, and successful aging among older adults. This qualitative study was used to examine the benefits of serious involvement in leisure activities among older Korean adults who were members of a sports club. Using an analytic data analysis, we identified three main themes associated with the benefits of serious engagement in leisure activities: 1) the experience of psychological benefits, 2) the creation of social support, and 3) the enhancement of physical health. These themes indicate that, through serious involvement in certain physical activities, participants gain various health benefits, which may contribute to successful aging. PMID:25059979

  12. Health benefits of serious involvement in leisure activities among older Korean adults

    Directory of Open Access Journals (Sweden)

    Junhyoung Kim

    2014-07-01

    Full Text Available The existing literature suggests that serious engagement in leisure activities leads to happiness, life satisfaction, and successful aging among older adults. This qualitative study was used to examine the benefits of serious involvement in leisure activities among older Korean adults who were members of a sports club. Using an analytic data analysis, we identified three main themes associated with the benefits of serious engagement in leisure activities: 1 the experience of psychological benefits, 2 the creation of social support, and 3 the enhancement of physical health. These themes indicate that, through serious involvement in certain physical activities, participants gain various health benefits, which may contribute to successful aging.

  13. Avaliação dos estágios extracurriculares de medicina em unidade de terapia intensiva adulto Evaluation of extracurricular internships in the adult's intensive care units

    Directory of Open Access Journals (Sweden)

    Diego Teixeira Nascimento

    2008-12-01

    to detect changes in attitude and interest of students who concluded these internships as well as the most frequent activities developed. METHODS: Descriptive cross-sectional survey conducted with students who did extracurricular internships in adult intensive care units during the second semester of 2006. A self-administered questionnaire was given using objective questions. RESULTS: We evaluated 49 students. Interest in becoming an intensivist was classified as high/very high by 32.7% before internship, after which 61.2% reported increased interest. Before internship, students on a 1 to 5 scale rated the importance of critical care medicine as 4.55 ± 0.70. After internship, 98% felt more confident to refer a patient to the intensive care unit, 95.9% to evaluate with supervision, patients admitted to intensive care units and 89.8% to attend patients in the emergency room. The most common procedures observed were: central venous access (100%, peripheral venous access (91.8% and orotracheal intubation (91.8%. Topics ranked in terms of interest from 1 to 5 were: systemic inflammatory response syndrome/sepsis (4.82 ± 0.48, shock (4.81 ± 0.44 and cardiopulmonary resuscitation (4.77 ± 0.55. CONCLUSIONS: This study showed that internships in adult intensive care units of Salvador (BA, Brazil provided students with greater assurance to evaluate critical patients, increased their interest to follow an intensivist physician career and allowed contact with the main procedures and topics related to critical care medicine.

  14. Activităţile academice extracurriculare, performanţele şcolare şi angajabilitatea masteranzilor

    Directory of Open Access Journals (Sweden)

    Octavian MOLDOVAN

    2013-12-01

    Full Text Available Although universities use Extracurricular Academic Activities (EAA as a marketing technique to attract students and boost their ratings vis-à-vis potential employers, their actual effects (positive or negative are still debated. This research explored (both theoretically and empirically the relationships between EAA, academic performances and employability (the perceived likelihood of graduates to find a job. According to quantitative empirical data, participation in EAA improves academic performances and although participation in EAA requires the same amount of time as normal coursework, the two activities seem to be in accordance with each other. Referring to the future, most respondents believe that universities should continuously develop EAA and are willing to recommend participation to their peers (believing that employers take into account participation in EAA when assessing candidates. The positive opinion regarding how EAA participation contributes toward finding a job can be explained by the perception that participation in EAA increases professional abilities, while EAA might represent a more convincing argument for employers than academic performances.Furthermore, the qualitative data shows that the professional and academic competencies (abilities improved by participating in AAE coincide with the expectations of potential employers and that the overall quality of academic programs could be improved by institutionalizing EAA, multiplying and diversifying these activities, including strategic partners, and so on. 

  15. 五段滚动式病理生理学课外实验教学模式的构建与成效%Innovative five phases and rolling model and extra-curricular scientific activities in pathophysiology experimental course and its achievements

    Institute of Scientific and Technical Information of China (English)

    董伟华; 张常娥; 周萍; 魏东海; 孔天翰; 陆丽; 董晓先; 刘金保; 杨昌山; 赵灿国; 吴卫; 温冠媚

    2010-01-01

    目的 培养学生的科研创新能力,构建创新型病理生理学课外实验教学模式.方法 运用创新型五段滚动式培养方法,将课外实验教学分为动员引导、择优选拔、课题申报、课题实施及课题总结5个阶段.结果 自2002年以来,约300名学生参与五段滚动式教学,获得学院学生科研课题立项36项,发表学术科研论文26篇,参与申报专利2项,学生的科技作品在各级竞赛中获奖31项,其中1项获得"挑战杯"全国大学生课外学术科技作品竞赛二等奖.此外,相关指导教师也获得广州医学院学生课外科研活动指导工作奖励20余项.结论 采用创新型五段滚动式培养方法,成功地构建了创新型病理生理学课外实验教学模式,取得了非常显著的成效.%Objective To cultivate student's innovative scientific ability and to establish an innovative model of extra-curriculum experimental teaching. Methods By using of innovative five phases and rolling training method, extra-curriculum experimental course was divided into five stages, mobilization and guiding, merit-based selection, project application, project implementation and project conclusion. Results Since 2002, there are more than 300 students have taken part in the extra-curriculum experimental course, 36 student' s scientific research projects were completed, 26 papers were published and 2 patents have been applied. The student's academic science and technology works have won 31 awards and one project won the second prize of "The Challenge Cup" national university student extracurricular academic science and technology work competition. Conclusions An innovative model of extra-curriculum experimental teaching in pathophysiology is successfully constructed, and remarkable achievement were acquired by using this innovative five phases and rolling model.

  16. 40 CFR 13.5 - Claims involving criminal activities or misconduct.

    Science.gov (United States)

    2010-07-01

    ... misconduct. 13.5 Section 13.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL CLAIMS COLLECTION STANDARDS General § 13.5 Claims involving criminal activities or misconduct. (a) The Administrator will refer cases of suspected criminal activity or misconduct to the EPA Office of Inspector...

  17. Middle-Class Parental Involvement in the Summer Activities of Four Elementary Students: A Qualitative Study

    Science.gov (United States)

    Ballard, Iva B.; Chappell, Manya; Johnson, Susan; Ngassam, Marlise DePaul

    2013-01-01

    In this study, we explore middle-class parental involvement in summer activities of four elementary students. Many researchers discuss summer programs initiated by institutions, but fail to explain how parents' availability, experiences, and related criteria affect student summer activities. From our interviews, observations, and artifacts, we…

  18. Activities involving aeronautical, space science, and technology support for minority institutions

    Science.gov (United States)

    1993-01-01

    The Final Report addressed the activities with which the Interracial Council for Business Opportunity (ICBO) was involved over the past 12 months. ICBO was involved in the design and development of a CARES Student Tracking System Software (CARES). Cares is intended to provide an effective means of maintaining relevant current and historical information on NASA-funded students through a range of educational program initiatives. ICBP was extensively involved in the formation of a minority university consortium amd implementation of collaborative research activities by the consortium as part of NASA's Mission to Planet Earth/Earth Observing System. ICBO was involved in the formation of an HBCU/MI Consortium to facilitate technology transfer efforts to the small and minority business community in their respective regions.

  19. Involvement of Ca2+ Activated Cl- Channel Ano6 in Platelet Activation and Apoptosis

    Directory of Open Access Journals (Sweden)

    Guoxing Liu

    2015-11-01

    Full Text Available Background/Aims: The ubiquitously expressed Ca2+ Activated Cl- Channel Ano6 participates in the stimulation of cell membrane scrambling. Defective Ano6 underlies the Scott syndrome, an inherited bleeding disorder with impaired scrambling of plasma membrane phospholipids. At least in theory, the bleeding disorder of Scott syndrome may result from impaired platelet function. Activators of platelets include thrombin and collagen related peptide (CRP, which trigger increase of cytosolic Ca2+-activity ([Ca2+]i, production of reactive oxygen species (ROS, degranulation, integrin activation, as well as cell shrinkage and phospholipid scrambling of the cell membrane. The present study thus explored whether Ano6 modifies activation-induced alterations of cytosolic Ca2+-activity ([Ca2+]i, degranulation (P-selectin exposure, integrin activation, phosphatidylserine exposure on the platelet surface and platelet volume. Methods: Platelets from mice lacking Ano6 (ano6-/- were compared to platelets from corresponding wild-type mice (ano6+/+. [Ca2+]i was estimated from Fluo-3 fluorescence, ROS from DCFDA fluorescence, degranulation from P-selectin abundance, integrin activation from αIIbβ3-integrin abundance, phosphatidylserine abundance from annexin-V-binding, and cell volume from forward scatter. Results: Platelet number in blood was slightly higher in ano6-/- mice than in ano6+/+ mice. Without activation [Ca2+]i and volume were similar in ano6-/- and ano6+/+ platelets as well as ROS abundance, P-selectin abundance, αIIbβ3 integrin activation, and phosphatidylserine exposure were negligible in both genotypes. Thrombin (0.01 U/ml and CRP (2 or 5 µg/ml increased [Ca2+]i, ROS abundance, platelet degranulation, αIIbβ3 integrin activation, and triggered annexin-V-binding as well as cell shrinkage, all effects less pronounced in ano6-/- than in ano6+/+ platelets. Conclusions: Genetic knockout of Ano6 blunts thrombin- and CRP-induced activation and apoptosis

  20. Factors Involved in Iranian Women Heads of Household's Health Promotion Activities: A Grounded Theory Study.

    Science.gov (United States)

    Rafii, Forough; Seyedfatemi, Naima; Rezaei, Mahboubeh

    2013-01-01

    We aimed to explore and describe the factors involved in Iranian women heads of household's health promotion activities. Grounded theory was used as the method. Sixteen women heads of household were recruited. Data were generated by semi structured interviews. Our findings indicated that remainder of resources (money, time and energy) alongside perceived severity of health risk were two main factors whereas women's personal and socio-economic characteristics were two contextual factors involved in these women's health promotion activities. To help these women improve their health status, we recommended that the government, non-governmental organizations and health care professionals provide them with required resources and increase their knowledge by holding training sessions.

  1. Active serine involved in the stabilization of the active site loop in the Humicola lanuginosa lipase

    DEFF Research Database (Denmark)

    Peters, Günther H.j.; Svendsen, A.; Langberg, H.;

    1998-01-01

    reveal that the hinges of the active site lid are more flexible in the wild-type Hll than in S146A. In contrast, larger fluctuations are observed in the middle region of the active site loop in S 146A than in Hll. These findings reveal that the single mutation (S146A) of the active site serine leads......We have investigated the binding properties of and dynamics in Humicola lanuginosa lipase (HII) and the inactive mutant S146A (active Ser146 substituted with Ala) using fluorescence spectroscopy and molecular dynamics simulations, respectively. Hll and S146A show significantly different binding......, whereas only small changes are observed for I-Ill suggesting that the active site Lid in the latter opens more easily and hence more lipase molecules are bound to the liposomes. These observations are in agreement with molecular dynamics simulations and subsequent essential dynamics analyses. The results...

  2. Active Involvement of End Users When Developing Web-Based Mental Health Interventions.

    Science.gov (United States)

    de Beurs, Derek; van Bruinessen, Inge; Noordman, Janneke; Friele, Roland; van Dulmen, Sandra

    2017-01-01

    Although many web-based mental health interventions are being released, the actual uptake by end users is limited. The marginal level of engagement of end users when developing these interventions is recognized as an important cause for uptake problems. In this paper, we offer our perceptive on how to improve user engagement. By doing so, we aim to stimulate a discourse on user involvement within the field of online mental health interventions. We shortly describe three different methods (the expert-driven method, intervention mapping, and scrum) that were currently used to develop web-based health interventions. We will focus to what extent the end user was involved in the developmental phase, and what the additional challenges were. In the final paragraph, lessons learned are summarized, and recommendations provided. Every method seems to have its trade-off: if end users are highly involved, availability of end users and means become problematic. If end users are less actively involved, the product may be less appropriate for the end user. Other challenges to consider are the funding of the more active role of technological companies, and the time it takes to process the results of shorter development cycles. Thinking about user-centered design and carefully planning, the involvement of end users should become standard in the field of web-based (mental) health. When deciding on the level of user involvement, one should balance the need for input from users with the availability of resources such as time and funding.

  3. Some Views on Primary School Students' Extracurricular Reading%小学生课外阅读之我见

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

      独立自主的课外阅读对于小学生来说很重要,在传统教育模式下,学生习惯于被动地阅读并接受知识,没有自己的思想和见解。笔者对课外阅读的实际情况,提出几点建议,希望藉此让学生能够对课外阅读产生兴趣。%Independent extracurricular reading is very important for students, in the pattern of traditional education, students are accustomed to passively read and accept the knowledge, and do not have their own ideas and opinions. In view of the actual situ-ation of extracurricular reading, the writer puts forward some suggestions, hoping to let the students interested in extracurricu-lar reading.

  4. Evaluation of Analgesic Activity of Papaver libanoticum Extract in Mice: Involvement of Opioids Receptors

    Directory of Open Access Journals (Sweden)

    Mohamad Ali Hijazi

    2017-01-01

    Full Text Available Papaver libanoticum is an endemic plant to Lebanese region (family Papaveraceae that has not been investigated before. The present study aimed to explore the analgesic activity of dried ethanolic extract of Papaver libanoticum (PLE using tail flick, hot plate, and acetic acid induced writhing models in mice. The involvement of opioid receptors in the analgesic mechanism was investigated using naloxone antagonism. Results demonstrated that PLE exhibited a potent dose dependent analgesic activity in all tested models for analgesia. The analgesic effect involved activation of opioid receptors in the central nervous system, where both spinal and supraspinal components might be involved. The time course for analgesia revealed maximum activity after three hours in both tail flick and hot plate methods, which was prolonged to 24 hours. Metabolites of PLE could be responsible for activation of opioid receptors. The EC50 of PLE was 79 and 50 mg/kg in tail flick and hot plate tests, respectively. The total coverage of analgesia by PLE was double that of morphine in both tests. In conclusion, PLE proved to have opioid agonistic activity with a novel feature of slow and prolonged effect. The present study could add a potential tool in the armaments of opioid drugs as a natural potent analgesic and for treatment of opioid withdrawal syndrome.

  5. Evaluation of Analgesic Activity of Papaver libanoticum Extract in Mice: Involvement of Opioids Receptors

    Science.gov (United States)

    El-Mallah, Ahmed; Aboul-Ela, Maha; Ellakany, Abdalla

    2017-01-01

    Papaver libanoticum is an endemic plant to Lebanese region (family Papaveraceae) that has not been investigated before. The present study aimed to explore the analgesic activity of dried ethanolic extract of Papaver libanoticum (PLE) using tail flick, hot plate, and acetic acid induced writhing models in mice. The involvement of opioid receptors in the analgesic mechanism was investigated using naloxone antagonism. Results demonstrated that PLE exhibited a potent dose dependent analgesic activity in all tested models for analgesia. The analgesic effect involved activation of opioid receptors in the central nervous system, where both spinal and supraspinal components might be involved. The time course for analgesia revealed maximum activity after three hours in both tail flick and hot plate methods, which was prolonged to 24 hours. Metabolites of PLE could be responsible for activation of opioid receptors. The EC50 of PLE was 79 and 50 mg/kg in tail flick and hot plate tests, respectively. The total coverage of analgesia by PLE was double that of morphine in both tests. In conclusion, PLE proved to have opioid agonistic activity with a novel feature of slow and prolonged effect. The present study could add a potential tool in the armaments of opioid drugs as a natural potent analgesic and for treatment of opioid withdrawal syndrome. PMID:28280516

  6. Evaluation of Analgesic Activity of Papaver libanoticum Extract in Mice: Involvement of Opioids Receptors.

    Science.gov (United States)

    Hijazi, Mohamad Ali; El-Mallah, Ahmed; Aboul-Ela, Maha; Ellakany, Abdalla

    2017-01-01

    Papaver libanoticum is an endemic plant to Lebanese region (family Papaveraceae) that has not been investigated before. The present study aimed to explore the analgesic activity of dried ethanolic extract of Papaver libanoticum (PLE) using tail flick, hot plate, and acetic acid induced writhing models in mice. The involvement of opioid receptors in the analgesic mechanism was investigated using naloxone antagonism. Results demonstrated that PLE exhibited a potent dose dependent analgesic activity in all tested models for analgesia. The analgesic effect involved activation of opioid receptors in the central nervous system, where both spinal and supraspinal components might be involved. The time course for analgesia revealed maximum activity after three hours in both tail flick and hot plate methods, which was prolonged to 24 hours. Metabolites of PLE could be responsible for activation of opioid receptors. The EC50 of PLE was 79 and 50 mg/kg in tail flick and hot plate tests, respectively. The total coverage of analgesia by PLE was double that of morphine in both tests. In conclusion, PLE proved to have opioid agonistic activity with a novel feature of slow and prolonged effect. The present study could add a potential tool in the armaments of opioid drugs as a natural potent analgesic and for treatment of opioid withdrawal syndrome.

  7. Molecular genetic analysis of activation-tagged transcription factors thought to be involved in photomorphogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Neff, Michael M.

    2011-06-23

    This is a final report for Department of Energy Grant No. DE-FG02-08ER15927 entitled “Molecular Genetic Analysis of Activation-Tagged Transcription Factors Thought to be Involved in Photomorphogenesis”. Based on our preliminary photobiological and genetic analysis of the sob1-D mutant, we hypothesized that OBP3 is a transcription factor involved in both phytochrome and cryptochrome-mediated signal transduction. In addition, we hypothesized that OBP3 is involved in auxin signaling and root development. Based on our preliminary photobiological and genetic analysis of the sob2-D mutant, we also hypothesized that a related gene, LEP, is involved in hormone signaling and seedling development.

  8. Regrouping: organized activity involvement and social adjustment across the transition to high school.

    Science.gov (United States)

    Bohnert, Amy M; Aikins, Julie Wargo; Arola, Nicole T

    2013-01-01

    Although organized activities (OAs) have been established as important contexts of development, limited work has examined the role of OAs across the high school transition in buffering adolescents' social adjustment by providing opportunities for visibility and peer affiliation. The transition to high school is characterized by numerous changes and OAs may provide an important setting for establishing and maintaining peer relationships during this tumultuous time. This study included 151 8th grade U.S. students (58% male) who were assessed across the transition to high school (spring of 8th and 9th grade). Continuous involvement in academic activities across the transition and becoming involved (i.e., initiation) in community/service activities following the transition was associated with fewer depressive symptoms in the spring of 9th grade. Continuous involvement in sports and initiation of academic activities was associated with having more friendships. In addition, links between OAs and loneliness were only evident among females. There appear to be significant social benefits for OA involvement.

  9. Leisure Activity and Caregiver Involvement in Middle-Aged and Older Adults with Down Syndrome

    Science.gov (United States)

    Mihaila, Iulia; Hartley, Sigan L.; Handen, Benjamin L.; Bulova, Peter D.; Tumuluru, Rameshwari V.; Devenny, Darlynne A.; Johnson, Sterling C.; Lao, Patrick J.; Christian, Bradley, T.

    2017-01-01

    The present study examined leisure activity and its association with caregiver involvement (i.e., residence and time spent with primary caregiver) in 62 middle-aged and older adults with Down syndrome (aged 30-53 years). Findings indicated that middle-aged and older adults with Down syndrome frequently participated in social and passive leisure…

  10. Premature aging in mice activates a systemic metabolic response involving autophagy induction

    NARCIS (Netherlands)

    G. Mariño (Guillermo); A.P. Ugalde (Alejandro); N. Salvador-Montoliu (Natalia); I. Varela (Ignacio); P.M. Quirós (Pedro); J. Cadiñanos (Juan); I. van der Pluijm (Ingrid); J.M.P. Freije (José); C. López-Otín (Carlos)

    2008-01-01

    textabstractAutophagy is a highly regulated intracellular process involved in the turnover of most cellular constituents and in the maintenance of cellular homeostasis. It is well-established that the basal autophagic activity of living cells decreases with age, thus contributing to the accumulation

  11. Active involvement of people with intellectual disabilities in health research - A structured literature review

    NARCIS (Netherlands)

    Frankena, T.K.; Naaldenberg, J.; Cardol, M.; Linehan, C.; Schrojenstein Lantman-de Valk, H.M. van

    2015-01-01

    Actively involving people with intellectual disabilities (ID) in health research, also known as inclusive health research, is increasingly popular. Currently, insight into experiences of this type of research is scarce. To gain insight into this topic, a structured literature review was conducted

  12. Structure and activity of lacustrine sediment bacteria involved in nutrient and iron cycles

    DEFF Research Database (Denmark)

    da Silva Martins, Gilberto Jorge; Terada, Akihiko; Ribeiro, Daniel C;

    2011-01-01

    Knowledge of the bacterial community structure in sediments is essential to better design restoration strategies for eutrophied lakes. In this regard, the aim of this study was to quantify the abundance and activity of bacteria involved in nutrient and iron cycling in sediments from four Azorean ...

  13. Programa de Actividad Física Extracurricular en Adolescentes con Sobrepeso u Obesidad

    OpenAIRE

    Miguel Tortosa-Martínez; Pedro Gil-Madrona; Juan Carlos Pastor-Vicedo; Onofre Contreras-Jordán

    2016-01-01

    Nuestro objetivo en este estudio es comprobar la eficacia de un programa de actividad físico-deportiva (PAF) extracurricular, en un grupo de adolescentes con sobrepeso obesidad, durante un período de 6 meses, respecto al consumo máximo de oxígeno, frecuencia cardíaca de recuperación, presión arterial e índice de grasa cintura/cadera, y ver las diferencias en cuanto al género. El estudio lo realizamos con 38 estudiantes de primer ciclo de secundaria, con un IMC superior al percentil 85. Los re...

  14. Salamander limb regeneration involves the activation of a multipotent skeletal muscle satellite cell population.

    Science.gov (United States)

    Morrison, Jamie I; Lööf, Sara; He, Pingping; Simon, András

    2006-01-30

    In contrast to mammals, salamanders can regenerate complex structures after injury, including entire limbs. A central question is whether the generation of progenitor cells during limb regeneration and mammalian tissue repair occur via separate or overlapping mechanisms. Limb regeneration depends on the formation of a blastema, from which the new appendage develops. Dedifferentiation of stump tissues, such as skeletal muscle, precedes blastema formation, but it was not known whether dedifferentiation involves stem cell activation. We describe a multipotent Pax7+ satellite cell population located within the skeletal muscle of the salamander limb. We demonstrate that skeletal muscle dedifferentiation involves satellite cell activation and that these cells can contribute to new limb tissues. Activation of salamander satellite cells occurs in an analogous manner to how the mammalian myofiber mobilizes stem cells during skeletal muscle tissue repair. Thus, limb regeneration and mammalian tissue repair share common cellular and molecular programs. Our findings also identify satellite cells as potential targets in promoting mammalian blastema formation.

  15. Efectos de un programa de actividad física extracurricular en niños de primer ciclo de ESO con sobrepeso y obesidad

    Directory of Open Access Journals (Sweden)

    Juan Carlos Pastor Vicedo

    2012-01-01

    Full Text Available Obesity and its impact are increasingly obvious in Spanish society. Thus, the purpose of this study was to investigate the effectiveness of an extracurricular intervention of physical and sports activities in order to improve students' body composition and fitness level between two groups of adolescences with overweight or obesity for a six-month period. Moreover, a comparison based on gender was also targeted in this intervention. The sample consisted of 38 students, seventeen boys and twenty-one girls, with BMI above the 85th percentile. Several fitness tests and anthropometric measurements were performed. The results showed significant improvements in the experimental group regarding their physical condition and body fat percentage compared to the control group who did not show any significant achievements. According to the comparison based on gender, within the experimental group, girls achieved more significant results both in their physical fitness and in their body fat percentage.

  16. Personal Involvement with Learning Disability Children: Activities Groups Can Do for Personal Involvement with Learning Disability Children thru Movement Education.

    Science.gov (United States)

    Smith, Elizabeth I.

    Described are perceptual motor activities in the areas of coordination, agility, strength, balance, and endurance for use with learning disabled children. Provided are a rationale for movement education and definitions of 10 terms such as laterality and endurance. A sequence of activities is provided for the following skills: ball bouncing, rope…

  17. THE EXTERNAL FACTORS OF STUDENTS’ INVOLVEMENT IN SPEAKING ACTIVITY AT SMP PROGRESIF BUMI SHALAWAT

    Directory of Open Access Journals (Sweden)

    M.A. Nurul Haikal

    2016-07-01

    Full Text Available This research is conducted to know what factors that influence the students’ involvement in speaking activity in order to practice their speaking skill and what strategies that the teacher used to encourage those external factors. This research uses descriptive qualitative method. There are two instruments used for this research, namely, class observation and interview. Based on the results of class observation and interview, the researcher concludes that teacher factor gives the greatest impact on students’ involvement and the appropriate strategies can support those external factors.

  18. Embedding a Recovery Orientation into Neuroscience Research: Involving People with a Lived Experience in Research Activity.

    Science.gov (United States)

    Stratford, Anthony; Brophy, Lisa; Castle, David; Harvey, Carol; Robertson, Joanne; Corlett, Philip; Davidson, Larry; Everall, Ian

    2016-03-01

    This paper highlights the importance and value of involving people with a lived experience of mental ill health and recovery in neuroscience research activity. In this era of recovery oriented service delivery, involving people with the lived experience of mental illness in neuroscience research extends beyond their participation as "subjects". The recovery paradigm reconceptualises people with the lived experience of mental ill health as experts by experience. To support this contribution, local policies and procedures, recovery-oriented training for neuroscience researchers, and dialogue about the practical applications of neuroscience research, are required.

  19. Definition of a Bidirectional Activity-Dependent Pathway Involving BDNF and Narp

    Directory of Open Access Journals (Sweden)

    Abigail Mariga

    2015-12-01

    Full Text Available One of the cardinal features of neural development and adult plasticity is the contribution of activity-dependent signaling pathways. However, the interrelationships between different activity-dependent genes are not well understood. The immediate early gene neuronal-activity-regulated pentraxin (NPTX2 or Narp encodes a protein that has been associated with excitatory synaptogenesis, AMPA receptor aggregation, and the onset of critical periods. Here, we show that Narp is a direct transcriptional target of brain-derived neurotrophic factor (BDNF, another highly regulated activity-dependent gene involved in synaptic plasticity. Unexpectedly, Narp is bidirectionally regulated by BDNF. Acute BDNF withdrawal results in downregulation of Narp, whereas transcription of Narp is greatly enhanced by BDNF. Furthermore, our results show that BDNF directly regulates Narp to mediate glutamatergic transmission and mossy fiber plasticity. Hence, Narp serves as a significant epistatic target of BDNF to regulate synaptic plasticity during periods of dynamic activity.

  20. Isolation of genes (nif/hup cosmids) involved in hydrogenase and nitrogenase activities in Rhizobium japonicum.

    Science.gov (United States)

    Hom, S S; Graham, L A; Maier, R J

    1985-03-01

    Recombinant cosmids containing a Rhizobium japonicum gene involved in both hydrogenase (Hup) and nitrogenase (Nif) activities were isolated. An R. japonicum gene bank utilizing broad-host-range cosmid pLAFR1 was conjugated into Hup- Nif- R. japonicum strain SR139. Transconjugants containing the nif/hup cosmid were identified by their resistance to tetracycline (Tcr) and ability to grow chemoautotrophically (Aut+) with hydrogen. All Tcr Aut+ transconjugants possessed high levels of H2 uptake activity, as determined amperometrically. Moreover, all Hup+ transconjugants tested possessed the ability to reduce acetylene (Nif+) in soybean nodules. Cosmid DNAs from 19 Hup+ transconjugants were transferred to Escherichia coli by transformation. When the cosmids were restricted with EcoRI, 15 of the 19 cosmids had a restriction pattern with 13.2-, 4.0-, 3.0-, and 2.5-kilobase DNA fragments. Six E. coli transformants containing the nif/hup cosmids were conjugated with strain SR139. All strain SR139 transconjugants were Hup+ Nif+. Moreover, one nif/hup cosmid was transferred to 15 other R. japonicum Hup- mutants. Hup+ transconjugants of six of the Hup- mutants appeared at a frequency of 1.0, whereas the transconjugants of the other nine mutants remained Hup-. These results indicate that the nif/hup gene cosmids contain a gene involved in both nitrogenase and hydrogenase activities and at least one and perhaps other hup genes which are exclusively involved in H2 uptake activity.

  1. Premature aging in mice activates a systemic metabolic response involving autophagy induction.

    Science.gov (United States)

    Mariño, Guillermo; Ugalde, Alejandro P; Salvador-Montoliu, Natalia; Varela, Ignacio; Quirós, Pedro M; Cadiñanos, Juan; van der Pluijm, Ingrid; Freije, José M P; López-Otín, Carlos

    2008-07-15

    Autophagy is a highly regulated intracellular process involved in the turnover of most cellular constituents and in the maintenance of cellular homeostasis. It is well-established that the basal autophagic activity of living cells decreases with age, thus contributing to the accumulation of damaged macromolecules during aging. Conversely, the activity of this catabolic pathway is required for lifespan extension in animal models such as Caenorhabditis elegans and Drosophila melanogaster. In this work, we describe the unexpected finding that Zmpste24-null mice, which show accelerated aging and are a reliable model of human Hutchinson-Gilford progeria, exhibit an extensive basal activation of autophagy instead of the characteristic decline in this process occurring during normal aging. We also show that this autophagic increase is associated with a series of changes in lipid and glucose metabolic pathways, which resemble those occurring in diverse situations reported to prolong lifespan. These Zmpste24(-/-) mice metabolic alterations are also linked to substantial changes in circulating blood parameters, such as leptin, glucose, insulin or adiponectin which in turn lead to peripheral LKB1-AMPK activation and mTOR inhibition. On the basis of these results, we propose that nuclear abnormalities causing premature aging in Zmpste24(-/-) mice trigger a metabolic response involving the activation of autophagy. However, the chronic activation of this catabolic pathway may turn an originally intended pro-survival strategy into a pro-aging mechanism and could contribute to the systemic degeneration and weakening observed in these progeroid mice.

  2. Barriers to involvement in physical activities of persons with mental illness.

    Science.gov (United States)

    Shor, Ron; Shalev, Anat

    2016-03-01

    Participating in physical activities could be essential for reducing the multiple risk factors for health problems that persons with severe mental illness (SMI) may suffer. However, people with SMI are significantly less active than the general population. To develop knowledge about factors related to the perceived barriers hindering this population's participation in physical activities and the benefits this participation would have, a study was conducted in Israel with 86 people with mental illness living in community mental health facilities prior to their participation in a health promotion program. A mixed method was implemented and included: a scale designed to measure participants' perceptions of the barriers to and benefits of involvement in physical activities; instruments focusing on bio-psycho-social factors that may affect the level of barriers experienced; and personal interviews. The findings revealed high ranking for accessibility barriers hindering the participation in physical activities. Bio-psycho-social factors stemming from the participants' mental health, such as level of depression, were correlated with higher ranking of accessibility barriers. Bio-psycho-social factors reflecting positive mental health and health, such as positive appraisal of body weight, were correlated with lower ranking of accessibility barriers. Other barriers may include organizational and broader systemic barriers in the mental health facilities where the participants reside. These findings illuminate the need to consider the unique challenges that persons with mental illness may face in any attempt to advance their involvement in physical activity.

  3. College/school of pharmacy affiliation and community pharmacies' involvement in public health activities.

    Science.gov (United States)

    Westrick, Salisa C; Mount, Jeanine; Watcharadamrongkun, Suntaree

    2009-11-12

    To examine the relationship between pharmacy college/school affiliation and community pharmacies' involvement in immunization and emergency preparedness activities. Telephone interviews were completed with 1,704 community pharmacies randomly sampled from 17 states to determine the pharmacies' involvement in immunization promotion, vaccine distribution, in-house immunization delivery, and health emergency preparedness and response, affiliation with college/school of pharmacy, and selected pharmacy and public health-related characteristics. Pharmacy college/school-affiliated community pharmacies were more likely than non-affiliated pharmacies to participate in immunization and emergency preparedness when controlling for pharmacy characteristics. College/school affiliation generally became nonsignificant, however, when public health-related characteristics were included in the analysis. Affiliation with a college/school of pharmacy was related to community pharmacies' involvement in immunization and emergency preparedness.

  4. Suppression of pancreatic carcinoma growth by activating peroxisome proliferator-activated receptor γ involves angiogenesis inhibition

    Institute of Scientific and Technical Information of China (English)

    Yu-Wei Dong; Xing-Peng Wang; Kai Wu

    2009-01-01

    AIM: To study the possible actions and mechanisms of peroxisome proliferator-activated receptor γ (PPARγ), a ligand-activated transcription factor, in pancreatic carcinogenesis,especially in angiogenesis.METHODS: Expressions of PPARγ and retinoid acid receptor (RXRα) were examined by reverse-transcription polymerase chain reaction (RT-PCR) with immunocytochemical staining. Pancreatic carcinoma cells, PANC-1,were treated either with 9-cis-RA, a ligand of RXRα,or with 15-deoxy-Δ12,14 prostaglandin J2(15d-PGJ2), a ligand of PPARγ, or both. Antiproliferative effect was evaluated by cell viability using methyltetrazolium (MTT) assay. A pancreatic carcinoma xenograft tumor model of nude mice was established by inoculating PANC-1 cells subcutaneously. Rosiglitazone, a specific ligand of PPARγ, was administered via water drinking in experimental group of nude mice. After 75 d, all mice were sacrificed. Expression of proliferating cell nuclear antigen (PCNA) in tumor tissue was examined with immunohistochemical staining. Expression of vascular endothelial growth factor (VEGF) mRNA in PANC-1 cells, which were treated with 15d-PGJ2 or 9-cis-RA at variousconcentrations or different duration, was detected by semi-quantitative RT-PCR. Effects of Rosiglitazone on changes of microvascular density (MVD) and VEGF expression were investigated in xenograft tumor tissue. Neovasculature was detected with immunohistochemistry staining labeled with anti-Ⅳ collagen antibody, and indicated by MVD.RESULTS: RT-PCR and immunocytochemical staining showed that PPARγ and RXRα were expressed in PANC-1 cells at both transcription level and translation level. MTT assay demonstrated that 15d-PGJ2, 9-cis-RA and their combination inhibited the growth of PANC-1 cells in a dose-dependent manner. 9-cis-RA had a combined inhibiting action with 15d-PGJ2 on the growth of pancreatic carcinoma. In vivo studies revealed that Rosiglitazone significantly suppressed the growth of pancreatic carcinoma

  5. Benefits of extracurricular participation in dissection in a prosection-based medical anatomy program.

    Science.gov (United States)

    Whelan, Alexander; Leddy, John J; Ramnanan, Christopher J

    2017-09-07

    The purpose of this study was to evaluate the extracurricular cadaveric dissection program available to medical students at an institution with a modern (time-compressed, student-centered, and prosection-based) approach to medical anatomy education. Quantitative (Likert-style questions) and qualitative data (thematic analysis of open-ended commentary) were collated from a survey of three medical student cohorts who had completed preclerkship. Perceived benefits of dissection included the hands-on learning style and the development of anatomy expertise, while the main barrier that limited participation was the time-intensive nature of dissection. Despite perceived benefits, students preferred that dissection remain optional. Analysis of assessments for the MD2016 cohort revealed that dissection participation was associated with enhanced performance on anatomy items in each systems-based unit examination, with the largest benefits observed on discriminating items that assessed knowledge application. In conclusion, this study revealed that there are academic and perceived benefits of extracurricular participation in dissection. While millennial medical students recognized these benefits, these students also indicated strong preference for having flexibility and choice in their anatomy education, including the choice to participate in cadaveric dissection. Anat Sci Educ. © 2017 American Association of Anatomists. © 2017 American Association of Anatomists.

  6. Active Involvement of End Users When Developing Web-Based Mental Health Interventions

    Directory of Open Access Journals (Sweden)

    Derek de Beurs

    2017-05-01

    Full Text Available BackgroundAlthough many web-based mental health interventions are being released, the actual uptake by end users is limited. The marginal level of engagement of end users when developing these interventions is recognized as an important cause for uptake problems. In this paper, we offer our perceptive on how to improve user engagement. By doing so, we aim to stimulate a discourse on user involvement within the field of online mental health interventions.MethodsWe shortly describe three different methods (the expert-driven method, intervention mapping, and scrum that were currently used to develop web-based health interventions. We will focus to what extent the end user was involved in the developmental phase, and what the additional challenges were. In the final paragraph, lessons learned are summarized, and recommendations provided.ResultsEvery method seems to have its trade-off: if end users are highly involved, availability of end users and means become problematic. If end users are less actively involved, the product may be less appropriate for the end user. Other challenges to consider are the funding of the more active role of technological companies, and the time it takes to process the results of shorter development cycles.ConclusionThinking about user-centered design and carefully planning, the involvement of end users should become standard in the field of web-based (mental health. When deciding on the level of user involvement, one should balance the need for input from users with the availability of resources such as time and funding.

  7. Constitutively activated ERK sensitizes cancer cells to doxorubicin: Involvement of p53-EGFR-ERK pathway

    Indian Academy of Sciences (India)

    RATNA KUMARI; SURBHI CHOUHAN; SNAHLATA SINGH; RISHI RAJ CHHIPA; AMRENDRA KUMAR AJAY; MANOJ KUMAR BHAT

    2017-03-01

    The tumour suppressor gene p53 is mutated in approximately 50% of the human cancers. p53 is involved in genotoxicstress-induced cellular responses. The role of EGFR and ERK in DNA-damage-induced apoptosis is well known. Weinvestigated the involvement of activation of ERK signalling as a consequence of non-functional p53, in sensitivity ofcells to doxorubicin. We performed cell survival assays in cancer cell lines with varying p53 status: MCF-7 (wild-typep53, WTp53), MDA MB-468 (mutant p53, MUTp53), H1299 (absence of p53, NULLp53) and an isogenic cell lineMCF-7As (WTp53 abrogated). Our results indicate that enhanced chemosensitivity of cells lacking wild-type p53function is because of elevated levels of EGFR which activates ERK. Additionally, we noted that independent of p53status, pERK contributes to doxorubicin-induced cell death.

  8. Carbohydrate in the mouth enhances activation of brain circuitry involved in motor performance and sensory perception.

    Science.gov (United States)

    Turner, Clare E; Byblow, Winston D; Stinear, Cathy M; Gant, Nicholas

    2014-09-01

    The presence of carbohydrate in the human mouth has been associated with the facilitation of motor output and improvements in physical performance. Oral receptors have been identified as a potential mode of afferent transduction for this novel form of nutrient signalling that is distinct from taste. In the current study oral exposure to carbohydrate was combined with a motor task in a neuroimaging environment to identify areas of the brain involved in this phenomenon. A mouth-rinsing protocol was conducted whilst carbohydrate (CHO) and taste-matched placebo (PLA) solutions were delivered and recovered from the mouths of 10 healthy volunteers within a double-blind, counterbalanced design. This protocol eliminates post-oral factors and controls for the perceptual qualities of solutions. Functional magnetic resonance imaging of the brain was used to identify cortical areas responsive to oral carbohydrate during rest and activity phases of a hand-grip motor task. Mean blood-oxygen-level dependent signal change experienced in the contralateral primary sensorimotor cortex was larger for CHO compared with PLA during the motor task when contrasted with a control condition. Areas of activation associated with CHO exclusively were observed over the primary taste cortex and regions involved in visual perception. Regions in the limbic system associated with reward were also significantly more active with CHO. This is the first demonstration that oral carbohydrate signalling can increase activation within the primary sensorimotor cortex during physical activity and enhance activation of neural networks involved in sensory perception. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Mechanisms involved in VPAC receptors activation and regulation: lessons from pharmacological and mutagenesis studies.

    Directory of Open Access Journals (Sweden)

    Ingrid eLanger

    2012-10-01

    Full Text Available VIP plays diverse and important role in human physiology and physiopathology and their receptors constitute potential targets for the treatment of several diseases such as neurodegenerative disorder, asthma, diabetes and inflammatory diseases. This article reviews the current knowledge regarding the two VIP receptors, VPAC1 and VPAC2, with respect to mechanisms involved in receptor activation, G protein coupling, signaling, regulation and oligomerization.

  10. The influence of motivational involvement in physical activity level improving using formative evaluation procedures

    OpenAIRE

    Gonçalves, Francisco

    2014-01-01

    The current work was planned to build up an original motivational involvement, based on Self-Determination Theory and Social Cognitive Theory, to improving physical activity in disadvantaged adolescents. The theoretical structure has three main issues: social context (perceived independence, perceived social sustain, input and enjoyment); cognitive mediators (perceived option and self-efficacy); and motivational course (intrinsic motivation, commitment and positive self-concept). ...

  11. Activity and recovery cycles of National Rugby League matches involving higher and lower ranked teams.

    Science.gov (United States)

    Gabbett, Tim J

    2013-06-01

    This study investigated the influence of ladder position on ball-in-play and recovery periods in elite National Rugby League (NRL) competitive matches. Video recordings of 192 NRL matches and 18 NRL finals matches played over 2 competitive seasons were coded for activity and recovery cycles. Time when the ball was continuously in play was considered activity, whereas any stoppages during the match (e.g., for scrums, penalties, line drop outs, tries, and video referee decisions) were considered recovery. In comparison to matches involving lower standard teams, there was a greater proportion (effect size [ES] = 0.37-0.67) of long duration (>91 seconds) and a smaller proportion (ES = 0.49-0.68) of short duration (<45 seconds) ball-in-play periods when Top 4 teams were competing against other Top 4 teams. No meaningful differences were found between teams of different ladder positions for the proportion of short (ES = 0.04-0.16) and long (ES = 0.06-0.28) recovery periods. In comparison to fixture matches involving the top 4 teams, finals matches had a smaller proportion (ES = 0.56) of long duration activity periods, and a greater proportion (ES = 0.54) of short duration activity periods. Only small differences were found between finals matches and matches involving the Top 4 teams for the proportion of short (ES = 0.42) and long (ES = 0.41) recovery periods. These findings suggest that the competitive advantage of the best NRL teams is closely linked to their ability to maintain a higher playing intensity than less successful teams. Furthermore, long ball-in-play periods in high-standard fixture matches (i.e., involving the top 4 teams) ensure that players are adequately prepared for the ball-in-play demands of finals matches.

  12. Aluminum hydroxide adjuvant differentially activates the three complement pathways with major involvement of the alternative pathway.

    Science.gov (United States)

    Güven, Esin; Duus, Karen; Laursen, Inga; Højrup, Peter; Houen, Gunnar

    2013-01-01

    Al(OH)3 is the most common adjuvant in human vaccines, but its mode of action remains poorly understood. Complement involvement in the adjuvant properties of Al(OH)3 has been suggested in several reports together with a depot effect. It is here confirmed that Al(OH)3 treatment of serum depletes complement components and activates the complement system. We show that complement activation by Al(OH)3 involves the three major pathways by monitoring complement components in Al(OH)3-treated serum and in Al(OH)3-containing precipitates. Al(OH)3 activation of complement results in deposition of C3 cleavage products and membrane attack complex (MAC) and in generation of the anaphylatoxins C3a and C5a. Complement activation was time dependent and inhibited by chelation with EDTA but not EGTA+Mg(2+). We thus confirm that Al(OH)3 activates the complement system and show that the alternative pathway is of major importance.

  13. Aluminum hydroxide adjuvant differentially activates the three complement pathways with major involvement of the alternative pathway.

    Directory of Open Access Journals (Sweden)

    Esin Güven

    Full Text Available Al(OH3 is the most common adjuvant in human vaccines, but its mode of action remains poorly understood. Complement involvement in the adjuvant properties of Al(OH3 has been suggested in several reports together with a depot effect. It is here confirmed that Al(OH3 treatment of serum depletes complement components and activates the complement system. We show that complement activation by Al(OH3 involves the three major pathways by monitoring complement components in Al(OH3-treated serum and in Al(OH3-containing precipitates. Al(OH3 activation of complement results in deposition of C3 cleavage products and membrane attack complex (MAC and in generation of the anaphylatoxins C3a and C5a. Complement activation was time dependent and inhibited by chelation with EDTA but not EGTA+Mg(2+. We thus confirm that Al(OH3 activates the complement system and show that the alternative pathway is of major importance.

  14. Aluminum Hydroxide Adjuvant Differentially Activates the Three Complement Pathways with Major Involvement of the Alternative Pathway

    Science.gov (United States)

    Güven, Esin; Duus, Karen; Laursen, Inga; Højrup, Peter; Houen, Gunnar

    2013-01-01

    Al(OH)3 is the most common adjuvant in human vaccines, but its mode of action remains poorly understood. Complement involvement in the adjuvant properties of Al(OH)3 has been suggested in several reports together with a depot effect. It is here confirmed that Al(OH)3 treatment of serum depletes complement components and activates the complement system. We show that complement activation by Al(OH)3 involves the three major pathways by monitoring complement components in Al(OH)3-treated serum and in Al(OH)3-containing precipitates. Al(OH)3 activation of complement results in deposition of C3 cleavage products and membrane attack complex (MAC) and in generation of the anaphylatoxins C3a and C5a. Complement activation was time dependent and inhibited by chelation with EDTA but not EGTA+Mg2+. We thus confirm that Al(OH)3 activates the complement system and show that the alternative pathway is of major importance. PMID:24040248

  15. MR imaging in adults with Gaucher disease type I: evulation of marrow involvement and disease activity

    Energy Technology Data Exchange (ETDEWEB)

    Hermann, G. (Dept. of Radiology, Mount Sinai Medical Center, City Univ. of New York, NY (United States)); Shaprio, R.S. (Dept. of Radiology, Mount Sinai Medical Center, City Univ. of New York, NY (United States)); Abdelwahab, I.F. (Dept. of Radiology, Mount Sinai Medical Center, City Univ. of New York, NY (United States)); Grabowski, G. (Dept. of Pediatrics, Mount Sinai Medical Center, City Univ. of New York, NY (United States))

    1993-05-01

    An investigation was conducted to determine the usefulness of magnetic resonance imaging (MRI) in the evaluation of bone marrow involvement in patients with Gaucher disease type I. T1- and T2-weighted images were obtained of the lower extremities of 29 adult patients. Patients were classified into one of three groups based on marrow signal patterns on T1- and T2-weighted images as well as change in signal intensity from T1- to T2-weighted images. An increase in signal intensity from T1- to T2-weighted images was the criterion for an 'active process' within the bone marrow. Classification of the 29 patients produced the following results: Group A: Normal, 4 patients; group B: Marrow infiltration, 16 patients; group C: Marrow infiltration plus active marrow process, 9 patients. Correlation with clinical findings revealed that all nine patients with evidence of an active marrow process on MRI (group C) had acute bone pain. Conversely, only one of the remaining 20 patients (groups A and B) had bone pain. There was no correlation between disease activity and findings on conventional radiographs. We conclude the MRI provides an excellent noninvasive assessment of the extent and activity of marrow involvement in type I Gaucher disease. (orig.)

  16. Use of mass-media and active involvement in a national dental health campaign in Scotland

    DEFF Research Database (Denmark)

    Schou, L

    1987-01-01

    This paper describes the evaluation of a Dental Health Mass-Media Campaign directed at 5-7-yr-old children and their mothers. It aimed at increasing knowledge and awareness of dental health by making use of three different components: inserts in women's magazines; television commercial; material ...... that future national health education campaigns combine the mass-media approach to increase health awareness with active involvement activities to stimulate behavioural changes.......This paper describes the evaluation of a Dental Health Mass-Media Campaign directed at 5-7-yr-old children and their mothers. It aimed at increasing knowledge and awareness of dental health by making use of three different components: inserts in women's magazines; television commercial; material......, distinguished from the other two components by demanding an active involvement of the participants, had the largest impact. The television commercial, merely demanding a passive involvement of the participants, was less well remembered, and the magazine insert had the lowest recollection. It is suggested...

  17. Promoting Single-Parent Family Children's Attitudes toward Science and Science Performance through Extracurricular Science Intervention in Taiwan

    Science.gov (United States)

    Hong, Zuway-R.; Lin, Huann-shyang; Lawrenz, Frances

    2008-01-01

    This study investigated the efficacy of extracurricular science intervention in promoting students' science learning performance and attitudes toward science. The Junior High School Student Questionnaire (JSSQ) was used to measure attitudes toward science, sexist attitudes and perceptions of the classroom learning environment. Twenty-eight eighth…

  18. Relationship between Admission, Extracurricular Services and Academic Achievement in Secondary Schools: A Partial Least Square (PLS) Approach

    Science.gov (United States)

    Suleiman, Yusuf; Hanaf, Zahyah Bt; Bin Taslikhan, Muhajir

    2016-01-01

    This study investigates the nexus between two dimensions of student personnel services (Admission Services, Extracurricular Services) academic achievement in secondary schools with the particular interest to know the significant impact of these services on students' academic achievement. It is believed that the provision of these services are…

  19. Oatmeal Facials and Sock Wrestling: The Perils and Promises of Extra-Curricular Strategies for "Fixing" Boys' Education

    Science.gov (United States)

    Weaver-Hightower, Marcus B.

    2010-01-01

    Advocates for boys' education have forwarded numerous "fixes" for boys' supposed educational woes, including both academic and non-academic programs and strategies. This article focuses on the lesser-discussed non-academic, extra-curricular means suggested, specifically outdoor education. Using qualitative analysis of an Australian school's…

  20. Involvement of serotonin 2A receptor activation in modulating medial prefrontal cortex and amygdala neuronal activation during novelty-exposure

    DEFF Research Database (Denmark)

    Hervig, Mona El-Sayed; Jensen, Nadja Cecilie Hvid; Rasmussen, Nadja Bredo

    2017-01-01

    -field arena affects medial PFC activation and basolateral amygdala (BLA) reactivity. We used c-Fos immunoreactivity (IR) as a marker of neuronal activation and stereological quantification for obtaining the total number of c-Fos-IR neurons as a measure of regional activation. We further examined the impact...... of 5-HT2AR blockade on the striatal-projecting BLA neurons. Systemic administration of ketanserin (0.5 mg/kg) prior to novel open-field exposure resulted in reduced total numbers of c-Fos-IR cells in dorsomedial PFC areas and the BLA. Moreover, there was a positive correlation between the relative time......-treated animals, upholding its involvement in modulating averseness. Ketanserin did not affect the number of activated striatal-projecting BLA neurons (measured by number of Cholera Toxin b (CTb) retrograde labelled neurons also being c-Fos-IR) following CTb injection in the ventral striatum. These results...

  1. Cholinesterase Activity in Health Workers Involved in Handling and Spraying of Organophosphorous Insecticides

    Directory of Open Access Journals (Sweden)

    Himanshu Madaan

    2011-04-01

    Full Text Available Background: Recent era of agricultural boon is partially the result of extensive use of insecticides and pesticides. But these compounds also have potential to significantly alter the ecosystem and can cause acute poisonings as well as long term detrimental health effects in humans. These compounds can cause toxicity through all routes of exposure. They exert their effect mainly by the inhibition of Acetylcholinesterase (AC hE, which functions by removing acetylcholine (AC h from its postsynaptic receptors. Aims and Objectives: Human exposure to Organophosphorous (OP pesticides can develop lowered cholinesterase levels. The aim of the present study was to estimate the plasma levels of AC hE in personnel involved in handling of these compounds, during the period of active spraying operations. Methodology: The present study was conducted on 38 subjects working as Multipurpose Health Workers (MPHW GROUP-1 and Field Workers (FW GROUP-2 involved in the actual spraying of the insecticides, in District Rohtak, Haryana, India to see the effect of exposure to OP insecticides. Plasma cholinesterase activity was measured by a kinetic method based on hydrolysis of butrylthiocholine. The data was statistically analyzed using SPSS statistical package (SPSS version 5.0. Results and interpretation: The mean cholinesterase activity in group-2 was lower by 27.76% than that in group-1 and this difference was statistically significant (p < 0.05. The workers involved in actual spraying of the insecticides had a more marked reduction in the levels of Plasma AC hE, compared to the workers involved only in the handling of the insecticides indicating that this group is at a bigger risk.

  2. Antinociceptive Activity of Methanol Extract of Muntingia calabura Leaves and the Mechanisms of Action Involved

    Directory of Open Access Journals (Sweden)

    M. H. Mohd. Sani

    2012-01-01

    Full Text Available Muntingia calabura L. (family Elaeocarpaceae has been traditionally used to relieve various pain-related ailments. The present study aimed to determine the antinociceptive activity of methanol extract of M. calabura leaves (MEMC and to elucidate the possible mechanism of antinociception involved. The in vivo chemicals (acetic acid-induced abdominal constriction and formalin-, capsaicin-, glutamate-, serotonin-induced paw licking test and thermal (hot plate test models of nociception were used to evaluate the extract antinociceptive activity. The extract (100, 250, and 500 mg/kg was administered orally 60 min prior to subjection to the respective test. The results obtained demonstrated that MEMC produced significant (P<0.05 antinociceptive response in all the chemical- and thermal-induced nociception models, which was reversed after pretreatment with 5 mg/kg naloxone, a non-selective opioid antagonist. Furthermore, pretreatment with L-arginine (a nitric oxide (NO donor, NG-nitro-L-arginine methyl esters (L-NAME; an inhibitor of NO synthase (NOS, methylene blue (MB; an inhibitor of cyclic-guanosine monophosphate (cGMP pathway, or their combination also caused significant (P<0.05 change in the intensity of the MEMC antinociception. In conclusion, the MEMC antinociceptive activity involves activation of the peripheral and central mechanisms, and modulation via, partly, the opioid receptors and NO/cGMP pathway.

  3. O estágio extracurricular na formação profissional: a opinião dos estudantes de fisioterapia The academic training for the professional education: the opinion of the Physiotherapy students

    Directory of Open Access Journals (Sweden)

    Ramon Távora Viana

    2012-12-01

    students affirmed to have participated of extracurricular internship. Out of these, 55 (90.2% were motivated by the necessity of acquiring experience; 53 (86.9% were performing therapeutic interventions in patients; and 13 (21.3% had 15 to 20 working hours. However, 36 academics (59.0% did work in disagreement with the rules of the Federal Council of Physical Therapy and Occupational Therapy. The students considered the extracurricular internship as a tool of improvement of their professional formation, because it allowed the practice of common physical therapist daily life activities procedures and techniques. There is a necessity for competent organizations to inspect these kind of activities, so that the students can practice tasks accordingly their qualification level.

  4. Evidence that intramolecular interactions are involved in masking the activation domain of transcriptional activator Leu3p.

    Science.gov (United States)

    Wang, D; Hu, Y; Zheng, F; Zhou, K; Kohlhaw, G B

    1997-08-01

    The Leu3 protein of Saccharomyces cerevisiae regulates the expression of genes involved in branched chain amino acid biosynthesis and in ammonia assimilation. It is modulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis. In the presence of alpha-isopropylmalate, Leu3p is a transcriptional activator. In the absence of the signal molecule, the activation domain is masked, and Leu3p acts as a repressor. The recent discovery that Leu3p retains its regulatory properties when expressed in mammalian cells (Guo, H., and Kohlhaw, G. B. (1996) FEBS Lett. 390, 191-195) suggests that masking and unmasking of the activation domain occur without the participation of auxiliary proteins. Here we present experimental support for this notion and address the mechanism of masking. We show that modulation of Leu3p is exceedingly sensitive to mutations in the activation domain. An activation domain double mutant (D872N/D874N; designated Leu3-dd) was constructed that has the characteristics of a permanently masked activator. Using separately expressed segments containing either the DNA binding domain-middle region or the activation domain of wild type Leu3p (or Leu3-dd) in a modified yeast two-hybrid system, we provide direct evidence for alpha-isopropylmalate-dependent interaction between these segments. Finally, we use the phenotype of Leu3-dd-containing cells (slow growth in the absence of added leucine) to select for suppressor mutations that map to the middle region of Leu3-dd. The properties of nine such suppressors further support the idea that masking is an intramolecular process and suggest a means for mapping the surface involved in masking.

  5. Acoustic input and efferent activity regulate the expression of molecules involved in cochlear micromechanics

    Directory of Open Access Journals (Sweden)

    Verónica eLamas

    2015-01-01

    Full Text Available Electromotile activity in auditory outer hair cells (OHCs is essential for sound amplification. It relies on the highly specialized membrane motor protein prestin, and its interactions with the cytoskeleton. It is believed that the expression of prestin and related molecules involved in OHC electromotility may be dynamically regulated by signals from the acoustic environment. However little is known about the nature of such signals and how they affect the expression of molecules involved in electromotility in OHCs. We show evidence that prestin oligomerization is regulated, both at short and relatively long term, by acoustic input and descending efferent activity originating in the cortex, likely acting in concert. Unilateral removal of the middle ear ossicular chain reduces levels of trimeric prestin, particularly in the cochlea from the side of the lesion, whereas monomeric and dimeric forms are maintained or even increased in particular in the contralateral side, as shown in Western blots. Unilateral removal of the auditory cortex (AC, which likely causes an imbalance in descending efferent activity on the cochlea, also reduces levels of trimeric and tetrameric forms of prestin in the side ipsilateral to the lesion, whereas in the contralateral side prestin remains unaffected, or even increased in the case of trimeric and tetrameric forms. As far as efferent inputs are concerned, unilateral ablation of the AC up-regulates the expression of α10 nAChR transcripts in the cochlea. Limited, unbalanced efferent activity after unilateral AC removal, also affects prestin and β-actin mRNA levels. These findings support that the concerted action of acoustic and efferent inputs to the cochlea is needed to regulate the expression of major molecules involved in OHC electromotility, both at the transcriptional and posttranscriptional levels.

  6. Anticancer activities of pterostilbene-isothiocyanate conjugate in breast cancer cells: involvement of PPARγ.

    Directory of Open Access Journals (Sweden)

    Kumar Nikhil

    Full Text Available Trans-3,5-dimethoxy-4'-hydroxystilbene (PTER, a natural dimethylated analog of resveratrol, preferentially induces certain cancer cells to undergo apoptosis and could thus have a role in cancer chemoprevention. Peroxisome proliferator-activated receptor γ (PPARγ, a member of the nuclear receptor superfamily, is a ligand-dependent transcription factor whose activation results in growth arrest and/or apoptosis in a variety of cancer cells. Here we investigated the potential of PTER-isothiocyanate (ITC conjugate, a novel class of hybrid compound (PTER-ITC synthesized by appending an ITC moiety to the PTER backbone, to induce apoptotic cell death in hormone-dependent (MCF-7 and -independent (MDA-MB-231 breast cancer cell lines and to elucidate PPARγ involvement in PTER-ITC action. Our results showed that when pre-treated with PPARγ antagonists or PPARγ siRNA, both breast cancer cell lines suppressed PTER-ITC-induced apoptosis, as determined by annexin V/propidium iodide staining and cleaved caspase-9 expression. Furthermore, PTER-ITC significantly increased PPARγ mRNA and protein levels in a dose-dependent manner and modulated expression of PPARγ-related genes in both breast cancer cell lines. This increase in PPARγ activity was prevented by a PPARγ-specific inhibitor, in support of our hypothesis that PTER-ITC can act as a PPARγ activator. PTER-ITC-mediated upregulation of PPARγ was counteracted by co-incubation with p38 MAPK or JNK inhibitors, suggesting involvement of these pathways in PTER-ITC action. Molecular docking analysis further suggested that PTER-ITC interacted with 5 polar and 8 non-polar residues within the PPARγ ligand-binding pocket, which are reported to be critical for its activity. Collectively, our observations suggest potential applications for PTER-ITC in breast cancer prevention and treatment through modulation of the PPARγ activation pathway.

  7. Involvement of Ca2+-activated K+ Channels in Receptor-Regulated Sperm Motility in Rats

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Previous voltage-clamp studies have demonstrated the modulation of sperm Ca2+-activated K+ (KCa) channels expressed in Xenopus oocytes by angiotensin Ⅱ (Ang Ⅱ)and extracellular ATP via AT1 receptor and P2U receptor, respectively. In the presentstudy, we investigated the involvement of KCa channels in receptor-regulated spermmotility of the rat using a computer-aided sperm analysis system, HTM-IVOS, in con-junction with Ca2+-mobilizing agents, receptor agonists/antagonists and KCa channelsblockers.The percentage of motile sperm was increased by ionomycin (0. 5 μmol/L), whichcould be inhibited by K+ channel blockers, tetraethylammonium (TEA 1 μmol/L ) orcharybdotoxin (ChTX, 300 nmol/L) indicating the presence of KCa channels. AngⅡ, at low concentration, 10 nmol/L, was found to increase motility, however, athigher concentration, 1 μmol/L, percentage of motility was found to be suppressed.Both stimulatory and inhibitory effects of Ang Ⅱ could be reversed by losartan, aspecific antagonist of AT 1 receptors, but not AT 2 antagonist PD123177, indicating theinvolvement of AT1 but not AT2 receptor in mediating both effects. ChTX also abol-ished both stimulatory and inhibitory effects of Ang H, suggesting the involvement ofKCa channels. The percentage of motility was also enhanced by extracellular ATP, afactor known to be involved in sperm activation. The ATP-enhanced sperm motilitywas mimicked by UTP , and inhibited by ChTX and reactive blue, an antagonist of P2receptor, indicating the involvement of both P2U and KCa channels. RT-PCR studywas also conducted to confirm the expression of KCa channels, AT1 receptors and P2Ureceptor, but not AT2 receptor, in rat caudal epididymal sperm. The present findingssuggest an important role of KCa channels in the regulation of sperm motility by AT1and P 2U receptors.

  8. Antimicrobial activity of Streptococcus salivarius K12 on bacteria involved in oral malodour.

    Science.gov (United States)

    Masdea, L; Kulik, E M; Hauser-Gerspach, I; Ramseier, A M; Filippi, A; Waltimo, T

    2012-08-01

    To investigate the antimicrobial activity of the bacteriocin-producing strain Streptococcus salivarius K12 against several bacteria involved in halitosis. The inhibitory activity of S. salivarius K12 against Solobacterium moorei CCUG39336, four clinical S. moorei isolates, Atopobium parvulum ATCC33793 and Eubacterium sulci ATCC35585 was examined by a deferred antagonism test. Eubacterium saburreum ATCC33271 and Parvimonas micra ATCC33270, which have been tested in previous studies, served as positive controls, and the Gram-negative strain Bacteroides fragilis ZIB2800 served as a negative control. Additionally, the occurrence of resistance in S. moorei CCUG39336 to S. salivarius K12 was analysed by either direct plating or by passage of S. moorei CCUG39336 on chloroform-inactived S. salivarius K12-containing agar plates. S. salivarius K12 suppressed the growth of all Gram-positive bacteria tested, but the extent to which the bacteria were inhibited varied. E. sulci ATCC35585 was the most sensitive strain, while all five S. moorei isolates were inhibited to a lesser extent. Natural resistance seems to be very low in S. moorei CCUG39336, and there was only a slight decrease in sensitivity after exposure to S. salivarius K12 over 10 passages. Our studies demonstrate that S. salivarius K12 has antimicrobial activity against bacteria involved in halitosis. This strain might be an interesting and valuable candidate for the development of an antimicrobial therapy for halitosis. Copyright © 2012 Elsevier Ltd. All rights reserved.

  9. Bacteroides gingivalis antigens and bone resorbing activity in root surface fractions of periodontally involved teeth

    Energy Technology Data Exchange (ETDEWEB)

    Patters, M.R.; Landsberg, R.L.; Johansson, L.A.; Trummel, C.L.; Robertson, P.R. (Department of Periodontology, University of Connecticut, School of Dental Medicine, Farmington, Connecticut, U.S.A.)

    1982-01-01

    Bone resorbing activity and the presence of antigens of Bacteroides gingivalis were assessed in plaque, calculus, cementum, and dentin obtained from roots of teeth previously exposed to periodontitis. Each fraction was obtained by scaling the root surface. The fraction were extracted by stirring and sonication, and the soluble centrifuged, sterilized, dialyzed, and adjusted to equivalent protein concentrations. Cementum and dentin extracts from impacted teeth were prepared similarly and served as controls. Stimulation of bone resorption by each extract was assessed in organ cultures of fetal rat bones by measurement of release of previously-incorporated /sup 45/Ca from the bone into the medium. In some groups of teeth, calculus and cementum were treated with acid prior to scaling. Citric acid washes were recovered and dialyzed. An enzyme-linked immunosorbent assay (ELISA) was used to assess the extracts for the presence of antigens reactive with an antiserum to B. gingivalis. Significant stimulation of bone resorption was found in all calculus and periodontally-involved cementum preparations. ELISA showed significant levels of B.gingivalis antigens in plaque, calculus, and cementum of periodontally-involved teeth, but not in involved dentin nor in cementum or dentin of impact teeth. Treatment with citric acid removed essentially all B.gingivalis antigens from cementum but not calculus. The results suggest that substances which stimulate bone resorption and substances which react with B. gingivalis antiserum are present in surface plaque, calculus, and cementum or periodontally-involved teeth. These substances are not present in cementum and dentin of impacted teeth nor in dentin of periodontally-involved teeth. Treatment by both scaling and citric demineralization will remove most of these substances from cementum of teeth previously exposed to periodontitis.

  10. Involvement of TACE in colon inflammation: a novel mechanism of regulation via SIRT-1 activation.

    Science.gov (United States)

    Sharma, Manoranjan; Mohapatra, Jogeswar; Wagh, Akshaya; Patel, Hiren M; Pandey, Dheerendra; Kadam, Shekhar; Argade, Anil; Deshpande, Shrikalp S; Shah, Gourang B; Chatterjee, Abhijit; Jain, Mukul R

    2014-03-01

    TNF-α converting enzyme (TACE) processes the membrane TNF-α to release the bioactive soluble TNF-α. Several evidences suggest the involvement of TNF-α and TACE in inflammatory bowel disease (IBD). Tissue inhibitor of metalloproteinase (TIMP)-3, an endogenous inhibitor of TACE, is positively associated with silent information regulator (SIRT)-1. We aimed to study the expression of TACE, TIMP-3 and SIRT-1 at different stages of colitis and how TACE is regulated in response to SIRT-1 activation. Acute colitis was induced by 3.5% dextran sulfate sodium (DSS) in drinking water for 5days and levels of cytokines and mRNA expression of TACE, TIMP-3 and SIRT-1 were measured in colon at different time intervals. Next, the effect of SIRT-1 activator (resveratrol) or a selective TACE inhibitor (compound 11p) treatment was evaluated. Elevated levels of TNF-α, interleukin (IL)-6, IL-1β, interferon (IFN)-γ and IL-17 were observed during DSS exposure phase which restored to the normal level after DSS removal. A significant increase in TACE and suppression in TIMP-3 and SIRT-1 mRNA level was observed during DSS exposure phase which reverts back to normal towards the remission phase. Treatment with resveratrol significantly elevated SIRT-1 and TIMP-3 and suppressed TACE mRNA expression and was associated with amelioration of disease. Furthermore, treatment with selective TACE inhibitor significantly suppressed body weight loss, disease activity index, colonic myeloperoxidase activity and the elevated levels of cytokines after DSS challenge. These results strongly emphasize the involvement of TACE in colon inflammation and inhibition of TACE directly or indirectly via SIRT-1 activation ameliorates colitis.

  11. Lipopolysaccharide-induced Lung Injury Involves the Nitration-mediated Activation of RhoA*

    Science.gov (United States)

    Rafikov, Ruslan; Dimitropoulou, Christiana; Aggarwal, Saurabh; Kangath, Archana; Gross, Christine; Pardo, Daniel; Sharma, Shruti; Jezierska-Drutel, Agnieszka; Patel, Vijay; Snead, Connie; Lucas, Rudolf; Verin, Alexander; Fulton, David; Catravas, John D.; Black, Stephen M.

    2014-01-01

    Acute lung injury (ALI) is characterized by increased endothelial hyperpermeability. Protein nitration is involved in the endothelial barrier dysfunction in LPS-exposed mice. However, the nitrated proteins involved in this process have not been identified. The activation of the small GTPase RhoA is a critical event in the barrier disruption associated with LPS. Thus, in this study we evaluated the possible role of RhoA nitration in this process. Mass spectroscopy identified a single nitration site, located at Tyr34 in RhoA. Tyr34 is located within the switch I region adjacent to the nucleotide-binding site. Utilizing this structure, we developed a peptide designated NipR1 (nitration inhibitory peptide for RhoA 1) to shield Tyr34 against nitration. TAT-fused NipR1 attenuated RhoA nitration and barrier disruption in LPS-challenged human lung microvascular endothelial cells. Further, treatment of mice with NipR1 attenuated vessel leakage and inflammatory cell infiltration and preserved lung function in a mouse model of ALI. Molecular dynamics simulations suggested that the mechanism by which Tyr34 nitration stimulates RhoA activity was through a decrease in GDP binding to the protein caused by a conformational change within a region of Switch I, mimicking the conformational shift observed when RhoA is bound to a guanine nucleotide exchange factor. Stopped flow kinetic analysis was used to confirm this prediction. Thus, we have identified a new mechanism of nitration-mediated RhoA activation involved in LPS-mediated endothelial barrier dysfunction and show the potential utility of “shielding” peptides to prevent RhoA nitration in the management of ALI. PMID:24398689

  12. Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA.

    Science.gov (United States)

    Rafikov, Ruslan; Dimitropoulou, Christiana; Aggarwal, Saurabh; Kangath, Archana; Gross, Christine; Pardo, Daniel; Sharma, Shruti; Jezierska-Drutel, Agnieszka; Patel, Vijay; Snead, Connie; Lucas, Rudolf; Verin, Alexander; Fulton, David; Catravas, John D; Black, Stephen M

    2014-02-21

    Acute lung injury (ALI) is characterized by increased endothelial hyperpermeability. Protein nitration is involved in the endothelial barrier dysfunction in LPS-exposed mice. However, the nitrated proteins involved in this process have not been identified. The activation of the small GTPase RhoA is a critical event in the barrier disruption associated with LPS. Thus, in this study we evaluated the possible role of RhoA nitration in this process. Mass spectroscopy identified a single nitration site, located at Tyr(34) in RhoA. Tyr(34) is located within the switch I region adjacent to the nucleotide-binding site. Utilizing this structure, we developed a peptide designated NipR1 (nitration inhibitory peptide for RhoA 1) to shield Tyr(34) against nitration. TAT-fused NipR1 attenuated RhoA nitration and barrier disruption in LPS-challenged human lung microvascular endothelial cells. Further, treatment of mice with NipR1 attenuated vessel leakage and inflammatory cell infiltration and preserved lung function in a mouse model of ALI. Molecular dynamics simulations suggested that the mechanism by which Tyr(34) nitration stimulates RhoA activity was through a decrease in GDP binding to the protein caused by a conformational change within a region of Switch I, mimicking the conformational shift observed when RhoA is bound to a guanine nucleotide exchange factor. Stopped flow kinetic analysis was used to confirm this prediction. Thus, we have identified a new mechanism of nitration-mediated RhoA activation involved in LPS-mediated endothelial barrier dysfunction and show the potential utility of "shielding" peptides to prevent RhoA nitration in the management of ALI.

  13. Involvement of multiple elements in FXR-mediated transcriptional activation of FGF19.

    Science.gov (United States)

    Miyata, Masaaki; Hata, Tatsuya; Yamakawa, Hiroki; Kagawa, Tatehiro; Yoshinari, Kouichi; Yamazoe, Yasushi

    2012-10-01

    The intestinal endocrine hormone human fibroblast growth factor 19 (FGF19) is involved in the regulation of not only hepatic bile acid metabolism but also carbohydrate and lipid metabolism. In the present study, bile acid/farnesoid X receptor (FXR) responsiveness in the FGF19 promoter region was investigated by a reporter assay using the human colon carcinoma cell line LS174T. The assay revealed the presence of bile acid/FXR-responsive elements in the 5'-flanking region up to 8.8 kb of FGF19. Deletion analysis indicated that regions from -1866 to -1833, from -1427 to -1353, and from -75 to +262 were involved in FXR responsiveness. Four, four, and two consecutive half-sites of nuclear receptors were observed in the three regions, respectively. An electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay revealed FXR/retinoid X receptor α (RXRα) heterodimer binding in these three regions. EMSA and reporter assays using mutated constructs indicated that the nuclear receptor IR1, ER2, and DR8 motifs in the 5'-flanking region were involved in FXR responsiveness of FGF19. Lithocholic acid (LCA) (10 μM), chenodeoxycholic acid (CDCA) (10 μM), or GW4064 (0.1 μM) treatment increased reporter activity in a construct including the three motifs under FXR-expressing conditions whereas LCA and not CDCA or GW4064 treatment increased the reporter activity under pregnane X receptor (PXR)-expressing conditions. These results suggest that FGF19 is transcriptionally activated through multiple FXR-responsive elements in the promoter region.

  14. Zymomonas mobilis Levan is Involved in Metalloproteinases Activation in Healing of Wounded and Burned Tissues

    Directory of Open Access Journals (Sweden)

    Cristina Sturzoiu

    2011-05-01

    Full Text Available Healing of burn tissue is a complete process involving reepitelization, granulation tissue formation and extracellular matrix remodeling. Thermal injury produces profound systemic changes, such as oligemic shock, anemia, renal failure and metabolic disorders. This causes direct tissue damages: inflammation and infection reactions. The tissue lesion also leads to increased oxidative stress in cells, as it has been observed by the low activity of endogenous antioxidant enzymatic and nonenzymatic systems. In this context, tissue matrix metalloproteinases (MMP plays a key role in normal physiology of conjunctive tissue during its development, morphogenesis or wound healing, having an irregular activity and being involved in the patho-physiological processes. The analysis of biological samples, MMP profiles contribute to the characterization of some processes involving tissue remodeling, processes related to wound or burn healing, possibly to the development of new therapies. In this context we studied the proliferative effect of levan, a polysaccharide produced by Gram negative bacteria, Zymomonas mobilis, a microorganism that plays an important role in modern biotechnology to produce substances of great interest in biotechnology, food industry or in biomedicine. Our studies focused on analysis of tissue MMPs profiles from Wistar rats with lesions caused by mechanic processes on skin (wounds and thermal (burn, treated by hallotherapy inCacica and Dej salt mines, before and after the treatment with levan. The results indicate that levan, a natural polysaccharide produced by wild type Z. mobilis NCIB 11163, as well as other bacterial strains, seems to have real value in the management of wounds and burns, applied individually or in combination with natural or artificial haloteraphy. The way that levan participates in the healing process is unknown, probably by activating the tissue metalloproteinases.

  15. Longitudinal relationships between perceived stress, exercise self-regulation and exercise involvement among physically active adolescents.

    Science.gov (United States)

    Gerber, Markus; Lindwall, Magnus; Brand, Serge; Lang, Christin; Elliot, Catherine; Pühse, Uwe

    2015-01-01

    Stress exposure may undermine exercisers' capability to self-regulate their exercise behaviour. This longitudinal study examined the interplay between perceived stress, exercise self-regulation (assessment of action and coping planning) and participation in vigorous exercise in vocational students. Moreover, this study examined whether high exercise self-regulation moderates the assumed negative relationship between stress and exercise. A sample of 580 physically active vocational students ([Formula: see text] ± s 17.8 ± 1.3 years, 33.8% girls) was assessed. All participants completed two identical validated questionnaires assessing stress, exercise self-regulation and exercise with a span of 10 months in between survey completion periods. The cross-sectional analyses show that high exercise self-regulation attenuated the assumed negative relationship between stress and exercise. In the longitudinal analyses, however, only a non-significant trend was found. Significant longitudinal relationships existed between exercise self-regulation and exercise involvement. Latent difference score models revealed that a drop in the exercise self-regulation was associated with a concurrent decrease in exercise participation. Cross-lagged panel analyses showed that high exercise self-regulation levels positively predicted exercise behaviour, but an inverse relationship was not supported. The findings suggested that higher exercise self-regulation levels were positively associated with future exercise involvement in currently active adolescents. While partial support was found that exercise self-regulation moderated the influence of stress on exercise, the findings demonstrated that higher exercise self-regulation levels had a positive impact on future exercise involvement in already active individuals.

  16. Engagement in school and community civic activities among rural adolescents.

    Science.gov (United States)

    Ludden, Alison Bryant

    2011-09-01

    Involvement in civic and community activities is a core part of positive youth development. Adolescents involved in voluntary civic activities have greater academic engagement, enhanced well-being, less involvement in problem behaviors, and they are more likely to value connections to their community than those who are not involved. The current research examined involvement in school and community civic activities as well as religious youth groups among 8th and 9th graders (N = 679, 61.7% female, 85.9% White) from small, rural schools in the Midwest U.S. and linked involvement to religiosity, well-being, problem behavior, academic engagement, and perceptions of parents and peers. Half of the adolescents in the sample reported involvement in civic activities or, more commonly, in religious youth groups. Adolescents who participated in religious youth groups reported more extracurriculars, less problem behavior, higher grades and motivation, and more support from parents and friends than adolescents who did not. The most frequently reported school civic activities were student council and Future Farmers of America, and 4-H was the most popular community civic activity. Those who were involved in school- and community-based civic activities reported more religiosity, academic engagement, and positive perceptions of parents and peers than uninvolved youth. The results support and extend research on rural youth by documenting civic activities across contexts and examining how involvement is associated with positive youth development.

  17. Helminth 2-Cys peroxiredoxin drives Th2 responses through a mechanism involving alternatively activated macrophages

    Science.gov (United States)

    Donnelly, Sheila; Stack, Colin M.; O'Neill, Sandra M.; Sayed, Ahmed A.; Williams, David L.; Dalton, John P.

    2008-01-01

    During helminth infections, alternatively activated macrophages (AAMacs) are key to promoting Th2 responses and suppressing Th1-driven inflammatory pathology. Th2 cytokines IL-4 and/or IL-13 are believed to be important in the induction and activation of AAMacs. Using murine models for the helminth infections caused by Fasciola hepatica (Fh) and Schistosoma mansoni (Sm), we show that a secreted antioxidant, peroxiredoxin (Prx), induces alternative activation of macrophages. These activated, Ym1-expressing macrophages enhanced the secretion of IL-4, IL-5, and IL-13 from naive CD4+ T cells. Administration of recombinant FhPrx and SmPrx to wild-type and IL-4−/− and IL-13−/− mice induced the production of AAMacs. In addition, Prx stimulated the expression of markers of AAMacs (particularly, Ym1) in vitro, and therefore can act independently of IL-4/IL-13 signaling. The immunomodulatory property of Prx is not due to its antioxidant activity, as an inactive recombinant variant with active site Cys residues replaced by Gly could also induce AAMacs and Th2 responses. Immunization of mice with recombinant Prx or passive transfer of anti-Prx antibodies prior to infection with Fh not only blocked the induction of AAMacs but also the development of parasite-specific Th2 responses. We propose that Prx activates macrophages as an initial step in the induction of Th2 responses by helminth parasites and is thereby a novel pathogen-associated molecular pattern.—Donnelly, S., Stack, C. M., O'Neill, S. M., Sayed, A. A., Williams, D. L., Dalton, J. P. Helminth 2-Cys peroxiredoxin drives Th2 responses through a mechanism involving alternatively activated macrophages. PMID:18708590

  18. Inhibition of fast axonal transport by pathogenic SOD1 involves activation of p38 MAP kinase.

    Directory of Open Access Journals (Sweden)

    Gerardo A Morfini

    Full Text Available Dying-back degeneration of motor neuron axons represents an established feature of familial amyotrophic lateral sclerosis (FALS associated with superoxide dismutase 1 (SOD1 mutations, but axon-autonomous effects of pathogenic SOD1 remained undefined. Characteristics of motor neurons affected in FALS include abnormal kinase activation, aberrant neurofilament phosphorylation, and fast axonal transport (FAT deficits, but functional relationships among these pathogenic events were unclear. Experiments in isolated squid axoplasm reveal that FALS-related SOD1 mutant polypeptides inhibit FAT through a mechanism involving a p38 mitogen activated protein kinase pathway. Mutant SOD1 activated neuronal p38 in mouse spinal cord, neuroblastoma cells and squid axoplasm. Active p38 MAP kinase phosphorylated kinesin-1, and this phosphorylation event inhibited kinesin-1. Finally, vesicle motility assays revealed previously unrecognized, isoform-specific effects of p38 on FAT. Axon-autonomous activation of the p38 pathway represents a novel gain of toxic function for FALS-linked SOD1 proteins consistent with the dying-back pattern of neurodegeneration characteristic of ALS.

  19. Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity

    Institute of Scientific and Technical Information of China (English)

    Byung Joo Kim; Jae Yeoul Jun; Insuk So; Ki Whan Kim

    2006-01-01

    AIM: Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity in cultured interstitial cells of Cajal.METHODS: Enzymatic digestions were used todissociate ICCs from the small intestine of a mouse. The whole-cell patch-clamp configuration was used to record membrane currents (voltage clamp) and potentials (current clamp) from cultured ICCs.RESULTS: Clonazepam and CGP37157 inhibited the pacemaking activity of ICCs in a dose-dependent manner.Clonazepam from 20 to 60 μmol/L and CGP37157 from 10 to 30 μmol/L effectively inhibited Ca2+ efflux from mitochondria in pacemaking activity of ICCs. The IC50S of clonazepam and CGP37157 were 37.1 and 18.2 μmol/L, respectively. The addition of 20 μmol/L NiCl2 to the internal solution caused a "wax and wane" phenomenon of pacemaking activity of ICCs.CONCLUSION: These results suggest that mitochondrial Na+-Ca2+ exchange has an important role in intestinal pacemaking activity.

  20. Involvement of phosphoinositide 3-kinases in neutrophil activation and the development of acute lung injury.

    Science.gov (United States)

    Yum, H K; Arcaroli, J; Kupfner, J; Shenkar, R; Penninger, J M; Sasaki, T; Yang, K Y; Park, J S; Abraham, E

    2001-12-01

    Activated neutrophils contribute to the development and severity of acute lung injury (ALI). Phosphoinositide 3-kinases (PI3-K) and the downstream serine/threonine kinase Akt/protein kinase B have a central role in modulating neutrophil function, including respiratory burst, chemotaxis, and apoptosis. In the present study, we found that exposure of neutrophils to endotoxin resulted in phosphorylation of Akt, activation of NF-kappaB, and expression of the proinflammatory cytokines IL-1beta and TNF-alpha through PI3-K-dependent pathways. In vivo, endotoxin administration to mice resulted in activation of PI3-K and Akt in neutrophils that accumulated in the lungs. The severity of endotoxemia-induced ALI was significantly diminished in mice lacking the p110gamma catalytic subunit of PI3-K. In PI3-Kgamma(-/-) mice, lung edema, neutrophil recruitment, nuclear translocation of NF-kappaB, and pulmonary levels of IL-1beta and TNF-alpha were significantly lower after endotoxemia as compared with PI3-Kgamma(+/+) controls. Among neutrophils that did accumulate in the lungs of the PI3-Kgamma(-/-) mice after endotoxin administration, activation of NF-kappaB and expression of proinflammatory cytokines was diminished compared with levels present in lung neutrophils from PI3-Kgamma(+/+) mice. These results show that PI3-K, and particularly PI3-Kgamma, occupies a central position in regulating endotoxin-induced neutrophil activation, including that involved in ALI.

  1. Histamine and spontaneous motor activity: biphasic changes, receptors involved and participation of the striatal dopamine system.

    Science.gov (United States)

    Chiavegatto, S; Nasello, A G; Bernardi, M M

    1998-01-01

    The time- and dose-related effects of exogenous histamine on spontaneous motor activity and receptors involved were evaluated in male rats. Intracerebroventricular administration of histamine (5.4 and 54.3 nmol) produced a biphasic effect with initial transitory hypoactivity and later hyperactivity expressed by locomotion frequency in an open-field. The rearing frequencies were only reduced by all doses of histamine used. The histamine-induced hypoactivity was inhibited by the H3-antagonist thioperamide and was also induced by the H3-agonist N-alpha-methylhistamine. The histamine-induced hyperactivity phase was blocked by the H1-antagonist mepyramine. The H2-antagonist ranitidine increased locomotion and rearing frequencies. The participation of other neurotransmitters in the persistent hypokinetic effect induced by 135.8 nmol of histamine was determined by HPLC in the striatum and hypothalamus as counter-proof. A decreased DOPAC/DA ratio was observed only in the striatum. In the hypothalamus, low levels of 5HT were detected, probably not correlated with motor activity. In conclusion, the present results suggest that the exogenous histamine-induced hypoactivity response is probably due to activation of H3-receptors as heteroreceptors reducing the activity of the striatal dopaminergic system. This effect can partially overlap with the expression of the hyperactivity induced by H1-receptor activation. The participation of H2-receptors requires further investigation.

  2. Changes in the activities of enzymes involved in the degradation of butylbenzyl phthalate by Pleurotus ostreatus.

    Science.gov (United States)

    Hwang, Soon-Seok; Kim, Hyoun-Young; Ka, Jong-Ok; Song, Hong-Gyu

    2012-02-01

    Degradation of butylbenzyl phthalate (BBP) by the white rot fungus Pleurotus ostreatus and the activities of some degrading enzymes were examined in two different media containing 100 mg/l of the compound. P. ostreatus pregrown for 7 days in complex YMG medium was able to completely degrade BBP within an additional 24 h but degraded only 35 mg/l of BBP in 5 days of incubation in minimal medium. Fungal cell mass in the culture in YMG medium was higher in the presence than in the absence of BBP. The esterase activity of the fungal culture in YMG medium was higher than that in minimal medium and increased with the addition of BBP. On the contrary, laccase activity was higher in minimal medium and it did not increase upon the addition of BBP. General peroxidase activity increased for a few days after the addition of BBP to both media. The degradation of BBP and its metabolites by P. ostreatus thus may be attributed mostly to esterase rather than lignin-degrading laccase. In addition, the activities of the enzymes involved in BBP degradation and their changes varied significantly in the different media and culture conditions.

  3. The benefits of in-group contact through physical activity involvement for health and well-being among Korean immigrants

    Directory of Open Access Journals (Sweden)

    Junhyoung Kim

    2014-05-01

    Full Text Available This qualitative study is designed to examine the benefits of physical activity involvement with members of the same ethnic group. For this study, Korean immigrants who were members of Korean physical activity clubs such as badminton and tennis were selected as participants. Using a constructive grounded theory methodology, three themes were identified as benefits of physical activity involvement: (1 the experience of psychological well-being, (2 the creation of a unique cultural world, and (3 the facilitation of physical activity involvement. The findings of this study suggest that Korean immigrant participants gained various social, cultural, and psychological benefits by engaging in activities with other Korean immigrants.

  4. The benefits of in-group contact through physical activity involvement for health and well-being among Korean immigrants

    Science.gov (United States)

    Kim, Junhyoung; Heo, Jinmoo; Kim, Jun

    2014-01-01

    This qualitative study is designed to examine the benefits of physical activity involvement with members of the same ethnic group. For this study, Korean immigrants who were members of Korean physical activity clubs such as badminton and tennis were selected as participants. Using a constructive grounded theory methodology, three themes were identified as benefits of physical activity involvement: (1) the experience of psychological well-being, (2) the creation of a unique cultural world, and (3) the facilitation of physical activity involvement. The findings of this study suggest that Korean immigrant participants gained various social, cultural, and psychological benefits by engaging in activities with other Korean immigrants. PMID:24875239

  5. El papel del maestro y de las actividades extracurriculares en la formacion de jovenes investigadores

    Directory of Open Access Journals (Sweden)

    Luis Fernando García M

    1996-04-01

    Full Text Available Este artículo presenta el texto de una conferencia dictada a un grupo de jóvenes universitarios identificados como investigadores promisorios dentro de las actividades de la Misión de Ciencia, Educación y Desarrollo. Se enfatiza, a partir de experiencias del autor, la importancia de que los jóvenes tengan contacto directo con verdaderos maestros de ciencia y con investigadores activos que les sirvan como modelos de identificación y paradigmas de vida. Igualmente se resalta la importancia de las actividades extracurriculares en la formación de los jóvenes con potencial investigativo, tanto a nivel de la educación secundaria como universitaria.

  6. EL PAPEL DEL MAESTRO Y DE LAS ACTIVIDADES EXTRACURRICULARES EN LA FORMACION DE JOVENES INVESTIGADORES

    Directory of Open Access Journals (Sweden)

    Luis Fernando García M.

    1996-01-01

    Full Text Available Este artículo presenta el texto de una conferencia dictada a un grupo de jóvenes universitarios identificados como investigadores promisorios dentro de las actividades de la Misión de Ciencia, Educación y Desarrollo. Se enfatiza, a partir de experiencias del autor, la importancia de que los jóvenes tengan contacto directo con verdaderos maestros de ciencia y con investigadores activos que les sirvan como modelos de identificación y paradigmas de vida. Igualmente se resalta la importancia de las actividades extracurriculares en la formación de los jóvenes con potencial investigativo, tanto a nivel

  7. Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C

    Institute of Scientific and Technical Information of China (English)

    Da-lei ZHANG; Kai-ming WANG; Cai-qiao ZHANG

    2009-01-01

    The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice.Spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA).After 72-h culture,Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached.Spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression.Ginsenosides (1.0~10 μg/ml) significantly stimulated proliferation of spermatogonia.Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10-8 to 107 mol/L and the PKC inhibitor H7 inhibited this effect.Likewise,ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H7.These results indicate that the proliferating effect ofginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway.

  8. Activation of an AMP-activated protein kinase is involved in post-diapause development of Artemia franciscana encysted embryos

    Directory of Open Access Journals (Sweden)

    Dai Jie-Qiong

    2009-03-01

    Full Text Available Abstract Background Cysts of Artemia can remain in a dormant state for long periods with a very low metabolic rate, and only resume their development with the approach of favorable conditions. The post-diapause development is a very complicated process involving a variety of metabolic and biochemical events. However, the intrinsic mechanisms that regulate this process are unclear. Results Herein we report the specific activation of an AMP-activated protein kinase (AMPK in the post-diapause developmental process of Artemia. Using a phospho-AMPKα antibody, AMPK was shown to be phosphorylated in the post-diapause developmental process. Results of kinase assay analysis showed that this phosphorylation is essential for AMPK activation. Using whole-mount immunohistochemistry, phosphorylated AMPK was shown to be predominantly located in the ectoderm of the early developed embryos in a ring shape; however, the location and shape of the activation region changed as development proceeded. Additionally, Western blotting analysis on different portions of the cyst extracts showed that phosphorylated AMPKα localized to the nuclei and this location was not affected by intracellular pH. Confocal microscopy analysis of immunofluorescent stained cyst nuclei further showed that AMPKα localized to the nuclei when activated. Moreover, cellular AMP, ADP, and ATP levels in developing cysts were determined by HPLC, and the results showed that the activation of Artemia AMPK may not be associated with cellular AMP:ATP ratios, suggesting other pathways for regulation of Artemia AMPK activity. Conclusion Together, we report evidence demonstrating the activation of AMPK in Artemia developing cysts and present an argument for its role in the development-related gene expression and energy control in certain cells during post-diapause development of Artemia.

  9. NRF2 activation is involved in ozonated human serum upregulation of HO-1 in endothelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Pecorelli, Alessandra [Department of Molecular and Developmental Medicine, University of Siena (Italy); Child Neuropsychiatry Unit, University Hospital, AOUS, Siena (Italy); Bocci, Velio [Department of Physiology, University of Siena (Italy); Acquaviva, Alessandra [Department of Molecular and Developmental Medicine, University of Siena (Italy); Belmonte, Giuseppe [Department of Biomedical Sciences, University of Siena (Italy); Gardi, Concetta [Department of Molecular and Developmental Medicine, University of Siena (Italy); Virgili, Fabio [INRAN, Rome (Italy); Ciccoli, Lucia [Department of Molecular and Developmental Medicine, University of Siena (Italy); Valacchi, Giuseppe, E-mail: giuseppe.valacchi@unife.it [Department of Life Sciences and Biotechnology, University of Ferrara (Italy); Department of Food and Nutrition, Kyung Hee University, Seoul (Korea, Republic of)

    2013-02-15

    During the last decade, it has been shown that the activation of NRF2 and the binding to electrophile-responsive element (EpREs), stimulates the expression of a great number of genes responsible for the synthesis of phase I and phase II proteins, including antioxidants enzymes and heme oxygenase-1 (HO-1). This critical cell response occurs in cardiovascular, degenerative and chronic infective diseases aggravated by a chronic oxidative stress. In our previous reports we have shown that ozonated plasma is able to up-regulate HO-1 expression in endothelial cells. In the present work we investigated a candidate mechanism involved in this process. After treatment with increasing doses of ozonated serum (20, 40 and 80 μg/mL O{sub 3} per mL of serum), a clear dose dependent activation of NRF2 and the subsequent induction of HO-1 and NAD(P)H quinone oxidoreductase 1(NQO1) was observed. This effect was also present when cells were treated with serum and hydrogen peroxide (H{sub 2}O{sub 2}) or serum and 4-hydroxynonenal (4HNE). Moreover, the treatment with ozonated serum was associated with a dose-dependent activation of extracellular-signal-regulated kinases (ERK1/2) and p38 MAP kinases (p38), not directly involved in NRF2 activation. These data, provide a new insight on the mechanism responsible for the induction of HO-1 expression by ozonated serum in the endothelium, and have a practical importance as an expedient approach to the treatment of patients with both effective orthodox drugs and ozonated autohemotherapy, targeted to the restoration of redox homeostasis. - Highlights: ► Endothelial HO1 is upregulated by ozonated plasma ► This activation is induced by NRF2 and it is ERK independent. ► 4HNE and H{sub 2}O{sub 2} are the main molecules involved in this process. ► Ozonated plasma induced a hormetic effect ► Combination of orthodox medicine and ozonated plasma can be a useful treatment.

  10. Cissus sicyoides: Pharmacological Mechanisms Involved in the Anti-Inflammatory and Antidiarrheal Activities

    Science.gov (United States)

    Beserra, Fernando Pereira; de Cássia Santos, Raquel; Périco, Larissa Lucena; Rodrigues, Vinicius Peixoto; de Almeida Kiguti, Luiz Ricardo; Saldanha, Luiz Leonardo; Pupo, André Sampaio; da Rocha, Lúcia Regina Machado; Dokkedal, Anne Lígia; Vilegas, Wagner; Hiruma-Lima, Clélia Akiko

    2016-01-01

    The objective of this study was to evaluate the pharmacological mechanisms involved in anti-inflammatory and antidiarrheal actions of hydroalcoholic extract obtained from the leaves of Cissus sicyoides (HECS). The anti-inflammatory effect was evaluated by oral administration of HECS against acute model of edema induced by xylene, and the mechanisms of action were analysed by involvement of arachidonic acid (AA) and prostaglandin E2 (PGE2). The antidiarrheal effect of HECS was observed and we analyzed the motility and accumulation of intestinal fluid. We also analyzed the antidiarrheal mechanisms of action of HECS by evaluating the role of the opioid receptor, α2 adrenergic receptor, muscarinic receptor, nitric oxide (NO) and PGE2. The oral administration of HECS inhibited the edema induced by xylene and AA and was also able to significantly decrease the levels of PGE2. The extract also exhibited significant anti-diarrheal activity by reducing motility and intestinal fluid accumulation. This extract significantly reduced intestinal transit stimulated by muscarinic agonist and intestinal secretion induced by PGE2. Our data demonstrate that the mechanism of action involved in the anti-inflammatory effect of HECS is related to PGE2. The antidiarrheal effect of this extract may be mediated by inhibition of contraction by acting on the intestinal smooth muscle and/or intestinal transit. PMID:26805827

  11. Transmitting Sport Values: The Importance of Parental Involvement in Children’s Sport Activity

    Science.gov (United States)

    Danioni, Francesca; Barni, Daniela; Rosnati, Rosa

    2017-01-01

    The transmission of positive values between parents and children is generally considered to be the hallmark of successful socialization. As this issue has been widely discussed but surprisingly little researched - especially with reference to core sport values - in this study we aimed to: 1) analyze adolescent athletes’ acceptance of the sport values their parents want to transmit to them (i.e., parental socialization values) and 2) examine the relationship between parental involvement in children’s sportive activity and adolescents’ acceptance of their parents’ socialization values. One hundred and seventy-two Italian adolescents (48.3% male, 51.7% female) who regularly practice team sports were asked to fill out a questionnaire which included the Youth Sport Values Questionnaire – 2 and the Parental Involvement in Sport Questionnaire. The dyadic correlations revealed that young athletes are in general willing to accept their parents’ socialization values in regards to sport. Moreover, from the relative weight analysis (a relatively new data analysis strategy), it emerged that parental involvement characterized by praise and understanding is the most important predictor of adolescents’ willingness to accept their parents’ sport values. Implications of these results and further expansion of the study are discussed. PMID:28344676

  12. Cissus sicyoides: Pharmacological Mechanisms Involved in the Anti-Inflammatory and Antidiarrheal Activities

    Directory of Open Access Journals (Sweden)

    Fernando Pereira Beserra

    2016-01-01

    Full Text Available The objective of this study was to evaluate the pharmacological mechanisms involved in anti-inflammatory and antidiarrheal actions of hydroalcoholic extract obtained from the leaves of Cissus sicyoides (HECS. The anti-inflammatory effect was evaluated by oral administration of HECS against acute model of edema induced by xylene, and the mechanisms of action were analysed by involvement of arachidonic acid (AA and prostaglandin E2 (PGE2. The antidiarrheal effect of HECS was observed and we analyzed the motility and accumulation of intestinal fluid. We also analyzed the antidiarrheal mechanisms of action of HECS by evaluating the role of the opioid receptor, α2 adrenergic receptor, muscarinic receptor, nitric oxide (NO and PGE2. The oral administration of HECS inhibited the edema induced by xylene and AA and was also able to significantly decrease the levels of PGE2. The extract also exhibited significant anti-diarrheal activity by reducing motility and intestinal fluid accumulation. This extract significantly reduced intestinal transit stimulated by muscarinic agonist and intestinal secretion induced by PGE2. Our data demonstrate that the mechanism of action involved in the anti-inflammatory effect of HECS is related to PGE2. The antidiarrheal effect of this extract may be mediated by inhibition of contraction by acting on the intestinal smooth muscle and/or intestinal transit.

  13. Polyamines and transglutaminase activity are involved in compatible and self-incompatible pollination of Citrus grandis.

    Science.gov (United States)

    Gentile, Alessandra; Antognoni, Fabiana; Iorio, Rosa Anna; Distefano, Gaetano; Las Casas, Giuseppina; La Malfa, Stefano; Serafini-Fracassini, Donatella; Del Duca, Stefano

    2012-02-01

    Pollination of pummelo (Citrus grandis L. Osbeck) pistils has been studied in planta by adding compatible and self-incompatible (SI) pollen to the stigma surface. The pollen germination has been monitored inside the pistil by fluorescent microscopy showing SI altered morphologies with irregular depositions of callose in the tube walls, and heavy callose depositions in enlarged tips. The polyamine (PA) content as free, perchloric acid (PCA)-soluble and -insoluble fractions and transglutaminase (TGase) activity have been analyzed in order to deepen their possible involvement in the progamic phase of plant reproduction. The conjugated PAs in PCA-soluble fraction were definitely higher than the free and the PCA-insoluble forms, in both compatible and SI pollinated pistils. In pistils, pollination caused an early decrease of free PAs and increase of the bound forms. The SI pollination, showed highest values of PCA-soluble and -insoluble PAs with a maximum in concomitance with the pollen tube arrest. As TGase mediates some of the effects of PAs by covalently binding them to proteins, its activity, never checked before in Citrus, was examined with two different assays. In addition, the presence of glutamyl-PAs confirmed the enzyme assay data and excluded the possibility of a misinterpretation. The SI pollination caused an increase in TGase activity, whereas the compatible pollination caused its decrease. Similarly to bound PAs, the glutamyl-PAs and the enzyme activity peaked in the SI pollinated pistils in concomitance with the observed block of the pollen tube growth, suggesting an involvement of TGase in SI response.

  14. CIPK23 is involved in iron acquisition of Arabidopsis by affecting ferric chelate reductase activity.

    Science.gov (United States)

    Tian, Qiuying; Zhang, Xinxin; Yang, An; Wang, Tianzuo; Zhang, Wen-Hao

    2016-05-01

    Iron deficiency is one of the major limiting factors affecting quality and production of crops in calcareous soils. Numerous signaling molecules and transcription factors have been demonstrated to play a regulatory role in adaptation of plants to iron deficiency. However, the mechanisms underlying the iron deficiency-induced physiological processes remain to be fully dissected. Here, we demonstrated that the protein kinase CIPK23 was involved in iron acquisition. Lesion of CIPK23 rendered Arabidopsis mutants hypersensitive to iron deficiency, as evidenced by stronger chlorosis in young leaves and lower iron concentration than wild-type plants under iron-deficient conditions by down-regulating ferric chelate reductase activity. We found that iron deficiency evoked an increase in cytosolic Ca(2+) concentration and the elevated Ca(2+) would bind to CBL1/CBL9, leading to activation of CIPK23. These novel findings highlight the involvement of calcium-dependent CBL-CIPK23 complexes in the regulation of iron acquisition. Moreover, mutation of CIPK23 led to changes in contents of mineral elements, suggesting that CBL-CIPK23 complexes could be as "nutritional sensors" to sense and regulate the mineral homeostasis in Arabisopsis.

  15. An update on renal involvement in hemophagocytic syndrome (macrophage activation syndrome).

    Science.gov (United States)

    Esmaili, Haydarali; Mostafidi, Elmira; Mehramuz, Bahareh; Ardalan, Mohammadreza; Mohajel-Shoja, Mohammadali

    2016-01-01

    Hemophagocytic syndrome (HPS) is mainly characterized by massive infiltration of bone marrow by activated macrophages and often presents with pancytopenia. Thrombotic microangiopathy (TMA) is also present with thrombocytopenia and renal involvement. Both conditions could coexist with each other and complicate the condition. Directory of Open Access Journals (DOAJ), EMBASE, Google Scholar, PubMed, EBSCO, and Web of Science with keywords relevant to; Hemophagocytic syndrome, macrophage activation syndrome, interferon-gamma and thrombotic microangiopathy, have been searched. Viral infection, rheumatologic disease and malignancies are the main underlying causes for secondary HPS. calcineurin inhibitors and viral infections are also the main underlying causes of TMA in transplant recipients. In this review, we discussed a 39-year-old male who presented with pancytopenia and renal allograft dysfunction. With the diagnosis of HPS induced TMA his renal condition and pancytopenia improved after receiving intravenous immunoglobulin (IVIG) and plasmapheresis therapy. HPS is an increasingly recognized disorder in the realm of different medical specialties. Renal involvement complicates the clinical picture of the disease, and this condition even is more complex in renal transplant recipients. We should consider the possibility of HPS in any renal transplant recipient with pancytopenia and allograft dysfunction. The combination of HPS with TMA future increases the complexity of the situation.

  16. Differences between Depressed and Non-Depressed Residents of Nursing Homes on Measures of Daily Activity Involvement and Affect.

    Science.gov (United States)

    Voelkl, Judith E.; Mathieu, Mary A.

    1993-01-01

    This study examined how depressed and nondepressed nursing home residents differed on measures of frequency of daily activity involvement and accompanying affect. Interviews indicated the groups differed significantly on frequency of activity involvement and affect. Depressed subjects spent large portions of time watching television. Nondepressed…

  17. Anticonvulsant activity of Dorema ammoniacum gum: evidence for the involvement of benzodiazepines and opioid receptors

    Science.gov (United States)

    Motevalian, Manijeh; Mehrzadi, Saeed; Ahadi, Samira; Shojaii, Asie

    2017-01-01

    This study investigated the anticonvulsant activity and possible mechanism of action of an aqueous solution of Dorema ammoniacum gum (DAG) which has been used traditionally in the treatment of convulsions. In this study, the anticonvulsant activity of DAG was examined using the pentylentetrazole (PTZ) model in mice. Thirty male albino mice were divided randomly and equally to 5 groups, and pretreated with normal saline, diazepam, or various doses of DAG (500, 700, and 1000 mg/kg, i.p.), prior to the injection of PTZ (60 mg/kg, i.p.). The latency and duration of seizures were recorded 30 min after PTZ injection. Pretreatments with naloxone and flumazenil in different groups were studied to further clarify the mechanisms of the anticonvulsant action. Phytochemical screening and thin layer chromatography (TLC) fingerprinting of ammoniacum gum was also determined. DAG showed significant anticonvulsant activity at all doses used. The gum delayed both the onset and the duration of seizures induced by PTZ. Treatment with flumazenil before DAG (700 mg/kg) inhibited the effect of gum on seizure duration and latency to some extent and administration of naloxone before DAG also significantly inhibited changes in latency and duration of seizure produced by DAG. The percentage inhibition was greater with naloxone than with flumazenil. This study showed that DAG had significant anticonvulsant activity in PTZ-induced seizures, and GABAergic and opioid systems may be involved. More studies are needed to further investigate its detailed mechanism. PMID:28255314

  18. Mitochondrial dysfunction is involved in the toxic activity of boric acid against Saprolegnia.

    Directory of Open Access Journals (Sweden)

    Shimaa E Ali

    Full Text Available There has been a significant increase in the incidence of Saprolegnia infections over the past decades, especially after the banning of malachite green. Very often these infections are associated with high economic losses in salmonid farms and hatcheries. The use of boric acid to control the disease has been investigated recently both under in vitro and in vivo conditions, however its possible mode of action against fish pathogenic Saprolegnia is not known. In this study, we have explored the transformation in Saprolegnia spores/hyphae after exposure to boric acid (1 g/L over a period 4-24 h post treatment. Using transmission electron microscopy (TEM, early changes in Saprolegnia spores were detected. Mitochondrial degeneration was the most obvious sign observed following 4 h treatment in about 20% of randomly selected spores. We also investigated the effect of the treatment on nuclear division, mitochondrial activity and function using confocal laser scanning microscopy (CLSM. Fluorescence microscopy was also used to test the effect of treatment on mitochondrial membrane potential and formation of reactive oxygen species. Additionally, the viability and proliferation of treated spores that correlated to mitochondrial enzymatic activity were tested using an MTS assay. All obtained data pointed towards changes in the mitochondrial structure, membrane potential and enzymatic activity following treatment. We have found that boric acid has no effect on the integrity of membranes of Saprolegnia spores at concentrations tested. It is therefore likely that mitochondrial dysfunction is involved in the toxic activity of boric acid against Saprolegnia spp.

  19. Mitochondrial dysfunction is involved in the toxic activity of boric acid against Saprolegnia.

    Science.gov (United States)

    Ali, Shimaa E; Thoen, Even; Evensen, Øystein; Wiik-Nielsen, Jannicke; Gamil, Amr A A; Skaar, Ida

    2014-01-01

    There has been a significant increase in the incidence of Saprolegnia infections over the past decades, especially after the banning of malachite green. Very often these infections are associated with high economic losses in salmonid farms and hatcheries. The use of boric acid to control the disease has been investigated recently both under in vitro and in vivo conditions, however its possible mode of action against fish pathogenic Saprolegnia is not known. In this study, we have explored the transformation in Saprolegnia spores/hyphae after exposure to boric acid (1 g/L) over a period 4-24 h post treatment. Using transmission electron microscopy (TEM), early changes in Saprolegnia spores were detected. Mitochondrial degeneration was the most obvious sign observed following 4 h treatment in about 20% of randomly selected spores. We also investigated the effect of the treatment on nuclear division, mitochondrial activity and function using confocal laser scanning microscopy (CLSM). Fluorescence microscopy was also used to test the effect of treatment on mitochondrial membrane potential and formation of reactive oxygen species. Additionally, the viability and proliferation of treated spores that correlated to mitochondrial enzymatic activity were tested using an MTS assay. All obtained data pointed towards changes in the mitochondrial structure, membrane potential and enzymatic activity following treatment. We have found that boric acid has no effect on the integrity of membranes of Saprolegnia spores at concentrations tested. It is therefore likely that mitochondrial dysfunction is involved in the toxic activity of boric acid against Saprolegnia spp.

  20. Neuronal processes involved in subjective feeling emergence: oscillatory activity during an emotional monitoring task.

    Science.gov (United States)

    Dan Glauser, Elise S; Scherer, Klaus R

    2008-06-01

    Subjective feeling, defined as the conscious experience of emotion and measured by self-report, is generally used as a manipulation check in studying emotional processes, rather than being the primary focus of research. In this paper, we report a first investigation into the processes involved in the emergence of a subjective feeling. We hypothesized that the oscillatory brain activity presumed to underlie the emergence of a subjective feeling can be measured by electroencephalographic (EEG) frequency band activity, similar to what has been shown in the literature for the conscious representation of objects. Emotional reactions were induced in participants using static visual stimuli. Episodes for which participants reported a subjective feeling were compared to those that did not lead to a conscious emotional experience, in order to identify potential differences between these two kinds of reactions at the oscillatory level. Discrete wavelet transforms of the EEG signal in gamma (31-63 Hz) and beta (15-31 Hz) bands showed significant differences between these two types of reactions. In addition, whereas beta band activities were widely distributed, differences in gamma band activity were predominantly observed in the frontal and prefrontal regions. The results are interpreted and discussed in terms of the complexity of the processes required to perform the affective monitoring task. It is suggested that future work on coherent mental representation of multimodal reaction patterns leading to the emergence of conscious emotional experience should include modifications in the time window examined and an extension of the frequency range to be considered.

  1. 长春市高校女大学生参加课外体育锻炼的现状及对策%Current Situation and Countermeasures of Female College Students Parti-cipating in Extracurricular Physical Exercise in Changchun Universities

    Institute of Scientific and Technical Information of China (English)

    张媛

    2016-01-01

    The female university students in Changchun university participate in extracurricular sports activities as the object, in the form of a questionnaire about Changchun City, female college students to participate in extracurricular sports activities in the frequency, motivation, attitude and influencing factors, gave the feasible countermeasures in view of the present situ-ation, in order to provide a theoretical basis for improving the extracurricular physical exercise of female college students in Colleges and universities in Changchun City, and to provide a basis for college sports curriculum theory research.%文章以长春高校女大学生参与课外体育活动作为对象,以调查问卷形式了解长春市高校女大学生参与课外体育活动的频度、动机、态度及影响因素,针对现状给予可行对策,为提高长春市高校女大学生课外体育锻炼提供理论依据,为高校体育课程理论研究提供依据。

  2. High School Sports Involvement Diminishes the Association Between Childhood Conduct Disorder and Adult Antisocial Behavior.

    Science.gov (United States)

    Samek, Diana R; Elkins, Irene J; Keyes, Margaret A; Iacono, William G; McGue, Matt

    2015-07-01

    Life course-persistent antisocial behavior manifests as a display of aggressive and antisocial behavior beginning in childhood (conduct disorder [CD]) and lasting through adulthood (adult antisocial personality disorder). This study aimed to build on prior research by evaluating whether involvement in high school sports helped attenuate the association between CD and subsequent adult antisocial behavior (AAB). A prospective sample of 967 male and female adolescents (56% adopted) was used. Structured interviews were used to assess CD (symptoms before the age of 15 years), involvement in sports during high school, and past-year adult antisocial personality disorder symptoms in young adulthood (M age = 22.4 years). As expected, the association between CD and AAB was significantly less for those involved in sports (β = .28; p sports (β = .49; p behavior in the model (age, gender, adoption status), and results were consistent across males and females. Involvement in other extracurricular activities (e.g., student government, plays, clubs) did not significantly moderate the relationship between CD and AAB. Although selection effects were evident (those with more CD symptoms were less likely to be involved in sports), findings nevertheless suggest high school sports involvement may be a notable factor related to disrupting persistent antisocial behavior beginning in childhood and adolescence and lasting through young adulthood. Implications are discussed. Copyright © 2015 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.

  3. UMA EXPERIÊNCIA EXTRACURRICULAR DE MATEMÁTICA COM CRIANÇAS CARENCIADAS. AN EXTRACURRICULAR EXPERIENCE OF MATHEMATICS WITH DEPRIVED CHILDREN

    Directory of Open Access Journals (Sweden)

    Carreira, Adelaide

    2012-05-01

    Full Text Available Descrevemos a concepção e a aplicação em Portugal de um projeto extracurricular – “O Continhas” – concebido e orientado simultaneamente para os alunos da pré-escola e dos quatro primeiros anos de escolaridade e para os seus respetivos docentes, o qual, em ambiente lúdico e descontraído, transmitisse às crianças participantes ideias, conceitos e métodos da matemática, procurando encorajar nelas a criação de pensamento lógico-abstrato e, em paralelo, interessá-las e aproximá-las, de maneira rigorosa, mas divertida, de uma disciplina que tanta resistência e rejeição costuma provocar. Instrumentos centrais do projeto são os seus “Objetos de Aprendizagem”, idealizados visando a aquisição de conhecimento conceptual e formal de matemática e o espertar da oralidade, do raciocínio abstrato e da capaciade de cálculo. Relatamos particularmente a experiência da aplicação do projeto em uma escola pública, inserida em uma zona ocupada por uma população imigrante de baixa renda. Em uma instituição com um aproveitamento escolar bastante inferior à media nacional, a matemática era alvo de um amplo desapreço; em um meio com sérias carências, comuns à instituição e ao alunado, os comportamentos desviantes faziam parte da experiência quotidiana. A aplicação do projeto promoveu alterações positivas nos procedimentos individuais e inter-pessoais dos alunos, implicando uma série de melhorias perceptíveis na sua conduta social e no seu dsempenho escolar global. We describe the conception and application in Portugal of an extra-curricular project – “O Continhas” – conceived and oriented for pre-school and primary school students and their respective teachers, which in a playful and relaxed atmosphere, transfers mathematical ideas, concepts and methods to the participating children, aiming the stimulation of their logical and abstract thinking, and, simultaneously, engaging their interest in and bringing

  4. Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains.

    Science.gov (United States)

    Sainz, Florencia; Jesús Torija, María; Matsutani, Minenosuke; Kataoka, Naoya; Yakushi, Toshiharu; Matsushita, Kazunobu; Mas, Albert

    2016-01-01

    Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the desired product composition. Moreover, the sequences of these genes were used to construct phylogenetic trees. According to the sequence of gcd, gene coding for mGDH, Acetobacter and Komagataeibacter

  5. Determination of dehydrogenase activities involved in D-glucose oxidation in Gluconobacter and Acetobacter strains

    Directory of Open Access Journals (Sweden)

    Florencia Sainz

    2016-08-01

    Full Text Available Acetic acid bacteria (AAB are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane bound dehydrogenases. In the present study, the enzyme activity of the membrane bound dehydrogenases (membrane-bound PQQ-glucose dehydrogenase (mGDH, D-gluconate dehydrogenase (GADH and membrane-bound glycerol dehydrogenase (GLDH involved in the oxidation of D-glucose and D-gluconic acid (GA was determined in six strains of three different species of AAB (three natural and three type strains. Moreover, the effect of these activities on the production of related metabolites (GA, 2-keto-D-gluconic acid (2KGA and 5-keto-D-gluconic acid (5KGA was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the A. malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h, which coincided with glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of G. oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the desired product composition.Moreover, the sequences of these genes were used to construct phylogenetic trees. According to the sequence of gcd, gene coding for mGDH, Acetobacter and Komagataeibacter were

  6. Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains

    Science.gov (United States)

    Sainz, Florencia; Jesús Torija, María; Matsutani, Minenosuke; Kataoka, Naoya; Yakushi, Toshiharu; Matsushita, Kazunobu; Mas, Albert

    2016-01-01

    Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the desired product composition. Moreover, the sequences of these genes were used to construct phylogenetic trees. According to the sequence of gcd, gene coding for mGDH, Acetobacter and Komagataeibacter

  7. Eph receptors are involved in the activity-dependent synaptic wiring in the mouse cerebellar cortex.

    Directory of Open Access Journals (Sweden)

    Roberta Cesa

    Full Text Available Eph receptor tyrosine kinases are involved in many cellular processes. In the developing brain, they act as migratory and cell adhesive cues while in the adult brain they regulate dendritic spine plasticity. Here we show a new role for Eph receptor signalling in the cerebellar cortex. Cerebellar Purkinje cells are innervated by two different excitatory inputs. The climbing fibres contact the proximal dendritic domain of Purkinje cells, where synapse and spine density is low; the parallel fibres contact the distal dendritic domain, where synapse and spine density is high. Interestingly, Purkinje cells have the intrinsic ability to generate a high number of spines over their entire dendritic arborisations, which can be innervated by the parallel fibres. However, the climbing fibre input continuously exerts an activity-dependent repression on parallel fibre synapses, thus confining them to the distal Purkinje cell dendritic domain. Such repression persists after Eph receptor activation, but is overridden by Eph receptor inhibition with EphA4/Fc in neonatal cultured cerebellar slices as well as mature acute cerebellar slices, following in vivo infusion of the EphA4/Fc inhibitor and in EphB receptor-deficient mice. When electrical activity is blocked in vivo by tetrodotoxin leading to a high spine density in Purkinje cell proximal dendrites, stimulation of Eph receptor activation recapitulates the spine repressive effects of climbing fibres. These results suggest that Eph receptor signalling mediates the repression of spine proliferation induced by climbing fibre activity in Purkinje cell proximal dendrites. Such repression is necessary to maintain the correct architecture of the cerebellar cortex.

  8. Involvement of BLT1 endocytosis and Yes kinase activation in leukotriene B4-induced neutrophil degranulation.

    Science.gov (United States)

    Gaudreault, Eric; Thompson, Charles; Stankova, Jana; Rola-Pleszczynski, Marek

    2005-03-15

    One of the important biological activities of human neutrophils is degranulation, which can be induced by leukotriene B4 (LTB4). Here we investigated the intracellular signaling events involved in neutrophil degranulation mediated by the high affinity LTB4 receptor, BLT1. Peripheral blood neutrophils as well as the promyeloid PLB-985 cell line, stably transfected with BLT1 cDNA and differentiated into a neutrophil-like cell phenotype, were used throughout this study. LTB4-induced enzyme release was inhibited by 50-80% when cells were pretreated with the pharmacological inhibitors of endocytosis sucrose, Con A and NH4Cl. In addition, transient transfection with a dominant negative form of dynamin (K44A) resulted in approximately 70% inhibition of ligand-induced degranulation. Pretreating neutrophils or BLT1-expressing PLB-985 cells with the Src family kinase inhibitor PP1 resulted in a 30-60% inhibition in BLT1-mediated degranulation. Yes kinase, but not c-Src, Fgr, Hck, or Lyn, was found to exhibit up-regulated kinase activity after LTB4 stimulation. Moreover, BLT1 endocytosis was found to be necessary for Yes kinase activation in neutrophils. LTB4-induced degranulation was also sensitive to inhibition of PI3K. In contrast, it was not affected by inhibition of the mitogen-activated protein kinase MEK kinase, the Janus kinases, or the receptor tyrosine kinase epidermal growth factor receptor or platelet-derived growth factor receptor. Taken together, our results suggest an essential role for BLT1 endocytosis and Yes kinase activation in LTB4-mediated degranulation of human neutrophils.

  9. Humor Appreciation Involves Parametric and Synchronized Activity in the Medial Prefrontal Cortex and Hippocampus.

    Science.gov (United States)

    Iidaka, Tetsuya

    2016-10-18

    Humor perception is a ubiquitous phenomenon in human societies. In theories of humor perception, three factors, non-seriousness, social context, and incongruity, have been implicated in humor. In another theory, however, elaboration and reinterpretation of contexts are considered to play a role in eliciting humor. Although the neural correlates of humor appreciation have been investigated using neuroimaging methods, only a few studies have conducted such experiments under natural conditions. In the present study, two functional magnetic resonance imaging experiments, using a comedy movie as a stimulus, were conducted to investigate the neural correlates of humor under natural conditions. The subjects' brain activity was measured while watching and enjoying a movie. In experiment 1, a parametric analysis showed that the medial prefrontal cortex (MPFC) and hippocampus/amygdala had a positive relationship with the subjective rating of funniness. In experiment 2, intersubject correlation was analyzed to investigate synchronized activity across all participants. Signal synchronization that paralleled increased funniness ratings was observed in the MPFC and hippocampus. Thus, it appears that both parametric and synchronized activity in the MPFC and hippocampus are important during humor appreciation. The present study has revealed the brain regions that are predominantly involved in humor sensation under natural condition. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  10. Astragaloside IV enhances diabetic wound healing involving upregulation of alternatively activated macrophages.

    Science.gov (United States)

    Luo, Xiaochun; Huang, Ping; Yuan, Baohong; Liu, Tao; Lan, Fang; Lu, Xiaoyan; Dai, Liangcheng; Liu, Yunjun; Yin, Hui

    2016-06-01

    Astragaloside IV (AS-IV), one of the major active compounds extracted from Astragali Radix, has been used experimentally for its potent antiinflammatory and immunoregulatory activities. In this study, we further investigate the potential efficacy of AS-IV on impaired wound healing in streptozotocin-induced diabetic mice. A full-thickness skin wound was produced on the back of diabetic mice and treated with AS-IV or vehicle topically. Our results showed that AS-IV application promoted diabetic wound repair with wounds gaping narrower and exhibiting augmented reepithelialization. AS-IV enhanced the collagen deposition and the expression of extracellular matrix (ECM)-related genes such as fibronectin and collagen IIIa, which implies a direct effect of AS-IV on matrix synthesis. AS-IV also improved the new blood vessel formation in wound tissue with increased numbers of endothelial cells and enhanced expression of VEGF and vWF. Moreover, the beneficial effect of AS-IV was related to the development of polarized alternatively activated macrophages, which involved in resolution of inflammation and facilitation of wound repair. All together, these findings suggest that AS-IV may play a potential effect on maintenance of cutaneous homeostasis and acceleration of diabetic wound healing.

  11. Protein receptor for activated C kinase 1 is involved in morphine reward in mice.

    Science.gov (United States)

    Wan, L; Su, L; Xie, Y; Liu, Y; Wang, Y; Wang, Z

    2009-07-07

    Opiate addiction is associated with upregulation of cAMP signaling in the brain. cAMP-responsive element binding protein (CREB), a nuclear transcription factor, is a downstream component of the extracellular signal-regulated protein kinase (ERK) pathway, which has been shown to regulate different physiological and psychological responses of drug addiction. RACK1, the protein receptor for activated C kinase 1, is a multifunctional scaffolding protein known to be a key regulator of various signaling cascades in the CNS. RACK1 functions specifically in integrin mediated activation of ERK cascade and targets active ERK. We examined if RACK1 is involved in the mechanism of drug addiction by regulating CREB in mouse hippocampus and prefrontal cortex. Several expressions were observed. Chronic administration of morphine made the expression of RACK1 and CREB mRNA increase in hippocampus and prefrontal cortex. The expression of RACK1 and CREB protein was strongly positive in CA1, CA3 and dentate gyrus (DG) of the hippocampus of morphine-treated mice brain, especially the pyramidal neurons in the DG of the hippocampus. Using the small interfering RNA technology, we determined that the expression of CREB mRNA was decreased in hippocampus and prefrontal cortex of morphine-treated mice. The expression of RACK1 and CREB protein was negative in CA1, CA3 and DG of hippocampus. These findings suggest that morphine reward can influence the expression of RACK1 in mouse hippocampus and prefrontal cortex through regulating CREB transcription.

  12. Involvement in sports clubs and informal sport activities of primary and secondary school children in Liechtenstein

    Directory of Open Access Journals (Sweden)

    Jürgen Kühnis

    2016-04-01

    Full Text Available Sport involvement among children and adolescents has been a central field of research in sport science since years. This paper documents the participation of 11- to 15-year-olds in sport clubs and informal sport activities in Liechtenstein and examines possible gender- and age-specific differences. The analysis is based on four cross-sectional studies from 2004 to 2015 and includes the data of 1’262 children in primary (5th grade and secondary (7th and 9th grades school. According to our findings sports and exercise are considered to be one of the main leisure-time activities for all school levels (irrespective of gender. The percentage of fully sport-abstinent adolescents by 11- and 13-year-olds is about 5 %; by 15-year-olds is around 10 %. The culmination of sports club membership (with current 84.7 % appears to be at the age of 11 (5th grade. After the switch to secondary school the sports club commitment tends to decrease, while the high attendance of the informal sport activities (>85 % shows relatively stable age development. In contrast to other child and youth studies, our data indicates a levelling tendency and dissolution of classic gender differences not only in sports club commitment but also in informal sports among girls and boys.

  13. Involvement of mitogen-activated protein kinase pathways in N-methyl-D-aspartate-induced excitotoxicity

    Institute of Scientific and Technical Information of China (English)

    Xiaorong Yang; Ping Sun; Huaping Qin; Rui Wang; Ye Wang; Ruihong Shi; Xin Zhao; Ce Zhang

    2011-01-01

    Previous studies have shown that mitogen-activated protein kinase (MAPK) signaling pathways are involved in N-methyl-D-aspartate (NMDA)-mediated excitotoxicity. However, a systematic observation or analysis of the role of these various MAPK pathways in excitotoxicity processes does not exist. The present study further evaluated the role and contribution of three MAPK pathways extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK in an NMDA-mediated excitotoxicity model using MAPK-specific inhibitor. Results demonstrated that c-Jun N-terminal kinase inhibitor SP600125 and/or p38 MAPK inhibitor SB203580 inhibited NMDA-induced reduction in cell viability, as well as reduced NMDA-induced lactate dehydrogenase leakage and reactive oxygen species production. However, PD98059, an inhibitor of extracellular signal-regulated kinase, did not influence this model. Results demonstrated an involvement of c-Jun N-terminal kinase and p38 MAPK, but not extracellular signal-regulated kinase, in NMDA-mediated excitotoxicity in cortical neurons.

  14. Activation of GPR15 and its involvement in the biological effects of smoking.

    Science.gov (United States)

    Kõks, Sulev; Kõks, Gea

    2017-06-01

    Smoking is one of the most significant modifiable environmental risk factors for many diseases. Smoking causes excessive mortality worldwide. Despite decades of long research, there has not been a clear understanding regarding the molecular mechanism that makes smoking harmful to health. Some recent studies have found that smoking influences most significantly the expression and methylation of GPR15. GPR15 is an orphan receptor that is involved in the regulation of the innate immunity and the T-cell trafficking in the intestinal epithelium. Further studies have confirmed that GPR15 is very strongly involved in smoking and smoking-induced molecular changes. Therefore, the altered expression and epigenetic regulation of GPR15 could have a significant role in the health impact of smoking. Impact statement The review describes an orphan receptor GPR15 that has recently been found to be influenced by smoking. This makes GPR15 very sensitive and adequate biomarker for smoking and smoking studies. Also, activation of GPR15 by smoking could help to explain its effects on health.

  15. Molecular Mechanisms Involved in the Antitumor Activity of Cannabinoids on Gliomas: Role for Oxidative Stress

    Energy Technology Data Exchange (ETDEWEB)

    Massi, Paola [Department of Pharmacology, Chemotherapy and Toxicology, University of Milan, Via Vanvitelli 32, 20129 Milan (Italy); Valenti, Marta; Solinas, Marta; Parolaro, Daniela, E-mail: daniela.parolaro@uninsubria.it [Department of Structural and Functional Biology, Section of Pharmacology, Center of Neuroscience, University of Insubria, Via A. da Giussano 10, 20152 Busto Arsizio, Varese (Italy)

    2010-05-26

    Cannabinoids, the active components of Cannabis sativa, have been shown to exert antiproliferative and proapoptotic effects on a wide spectrum of tumor cells and tissues. Of interest, cannabinoids have displayed great potency in reducing the growth of glioma tumors, one of the most aggressive CNS tumors, either in vitro or in animal experimental models curbing the growth of xenografts generated by subcutaneous or intrathecal injection of glioma cells in immune-deficient mice. Cannabinoids appear to be selective antitumoral agents as they kill glioma cells without affecting the viability of non-transformed cells. This review will summarize the anti-cancer properties that cannabinoids exert on gliomas and discuss their potential action mechanisms that appear complex, involving modulation of multiple key cell signaling pathways and induction of oxidative stress in glioma cells.

  16. Molecular Mechanisms Involved in the Antitumor Activity of Cannabinoids on Gliomas: Role for Oxidative Stress

    Directory of Open Access Journals (Sweden)

    Paola Massi

    2010-05-01

    Full Text Available Cannabinoids, the active components of Cannabis sativa, have been shown to exert antiproliferative and proapoptotic effects on a wide spectrum of tumor cells and tissues. Of interest, cannabinoids have displayed great potency in reducing the growth of glioma tumors, one of the most aggressive CNS tumors, either in vitro or in animal experimental models curbing the growth of xenografts generated by subcutaneous or intrathecal injection of glioma cells in immune-deficient mice. Cannabinoids appear to be selective antitumoral agents as they kill glioma cells without affecting the viability of non-transformed cells. This review will summarize the anti-cancer properties that cannabinoids exert on gliomas and discuss their potential action mechanisms that appear complex, involving modulation of multiple key cell signaling pathways and induction of oxidative stress in glioma cells.

  17. Getting involved in international development activities: UK initiatives and hidden benefits.

    Science.gov (United States)

    Cheeseborough, Jackie; Godbolt, Shane; Grant, Maria J

    2015-03-01

    Jackie Cheeseborough and Shane Godbolt describe the role that UK health information professionals have in global health and in supporting colleagues from developing countries to continue to develop as a provision. They give an overview of a range of organisations working to improve access to health information in developing countries and in particular Sub-Saharan Africa including Book Aid International, HIFA, INASP, ITOCA, Phi, TALC, THET and Research4Life. Even in a recession, many UK health librarians are choosing to get involved in international development activities in low-resource countries by volunteering, and discovering hidden benefits for their own organisations, and their own continuing professional development. © 2015 Health Libraries Journal.

  18. [Perception of fathers as for their involvement in activities with their children].

    Science.gov (United States)

    da Silva, Mara Regina Santos; Bueno, Maria Emilia Nunes; Ribeiro, Juliane Portella

    2014-03-01

    This is an exploratory, descriptive study with a quantitative approach and the aim to identzfy the perception of fathers regarding their children's needs; strategies used by fathers to get closer to their children as well as to analyze the influence of household chores and children's education in their fathers' lives. Study subjects were 92 men with six-year-old children, residing in the city of Rio Grande, state of Rio Grande do Sul (RS). The results revealed fathers who are more engaged in activities with their children, valuing confidence, safety, as well as proximity to family as children's main needs, having prioritized the progress children make as well as attention to listening and conversation. Children's education does not aggregate more problems than they imagined, managing to plan life the way they seek to. These findings show that, in the sample studied, fathers have been able to develop more affectionate actions aimed at being more involved with their children.

  19. Protein Folding Activity of the Ribosome is involved in Yeast Prion Propagation

    Science.gov (United States)

    Blondel, Marc; Soubigou, Flavie; Evrard, Justine; Nguyen, Phu hai; Hasin, Naushaba; Chédin, Stéphane; Gillet, Reynald; Contesse, Marie-Astrid; Friocourt, Gaëlle; Stahl, Guillaume; Jones, Gary W.; Voisset, Cécile

    2016-01-01

    6AP and GA are potent inhibitors of yeast and mammalian prions and also specific inhibitors of PFAR, the protein-folding activity borne by domain V of the large rRNA of the large subunit of the ribosome. We therefore explored the link between PFAR and yeast prion [PSI+] using both PFAR-enriched mutants and site-directed methylation. We demonstrate that PFAR is involved in propagation and de novo formation of [PSI+]. PFAR and the yeast heat-shock protein Hsp104 partially compensate each other for [PSI+] propagation. Our data also provide insight into new functions for the ribosome in basal thermotolerance and heat-shocked protein refolding. PFAR is thus an evolutionarily conserved cell component implicated in the prion life cycle, and we propose that it could be a potential therapeutic target for human protein misfolding diseases. PMID:27633137

  20. Protein Folding Activity of the Ribosome is involved in Yeast Prion Propagation.

    Science.gov (United States)

    Blondel, Marc; Soubigou, Flavie; Evrard, Justine; Nguyen, Phu Hai; Hasin, Naushaba; Chédin, Stéphane; Gillet, Reynald; Contesse, Marie-Astrid; Friocourt, Gaëlle; Stahl, Guillaume; Jones, Gary W; Voisset, Cécile

    2016-09-16

    6AP and GA are potent inhibitors of yeast and mammalian prions and also specific inhibitors of PFAR, the protein-folding activity borne by domain V of the large rRNA of the large subunit of the ribosome. We therefore explored the link between PFAR and yeast prion [PSI(+)] using both PFAR-enriched mutants and site-directed methylation. We demonstrate that PFAR is involved in propagation and de novo formation of [PSI(+)]. PFAR and the yeast heat-shock protein Hsp104 partially compensate each other for [PSI(+)] propagation. Our data also provide insight into new functions for the ribosome in basal thermotolerance and heat-shocked protein refolding. PFAR is thus an evolutionarily conserved cell component implicated in the prion life cycle, and we propose that it could be a potential therapeutic target for human protein misfolding diseases.

  1. A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments

    KAUST Repository

    Rodrigo, María J.

    2013-09-04

    Citrus is the first tree crop in terms of fruit production. The colour of Citrus fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C30 apocarotenoids, mainly ?-citraurin (3-hydroxy-?-apo-8?-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in Citrus fruits, little is known about the formation of C30 apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C30 apocarotenoids. In silico data mining revealed a new family of five CCD4-type genes in Citrus. One gene of this family, CCD4b1, was expressed in reproductive and vegetative tissues of different Citrus species in a pattern correlating with the accumulation of C30 apocarotenoids. Moreover, developmental processes and treatments which alter Citrus fruit peel pigmentation led to changes of ?-citraurin content and CCD4b1 transcript levels. These results point to the involvement of CCD4b1 in ?-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and ?-cryptoxanthin. Functional analysis of CCD4b1 by in vitro assays unequivocally demonstrated the asymmetric cleavage activity at the 7?,8? double bond in zeaxanthin and ?-cryptoxanthin, confrming its role in C30 apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7?,8? double bond of cyclic C40 carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C30 apocarotenoids in Citrus which are key pigments in fruit coloration. The Author 2013.

  2. Activation of Alveolar Macrophages after Plutonium Oxide Inhalation in Rats: Involvement in the Early Inflammatory Response

    Energy Technology Data Exchange (ETDEWEB)

    Van der Meeren, A.; Tourdes, F.; Gremy, O.; Grillon, G.; Abram, M.C.; Poncy, J.L.; Griffiths, N. [CEA, DSV, DRR, SRCA, Centre DAM Ile de France, F-91297 Bruyeres Le Chatel, Arpajon (France)

    2008-07-01

    Alveolar macrophages play an important role in the distribution, clearance and inflammatory reactions after particle inhalation, which may influence long-term events such as fibrosis and tumorigenesis. The objectives of the present study were to investigate the early inflammatory events after plutonium oxide inhalation in rats and involvement of alveolar macrophages. Lung changes were studied from 3 days to 3 months after inhalation of PuO{sub 2} or different isotopic compositions (70% or 97% {sup 239}Pu) and initial lung deposits (range 2.1 to 43.4 kBq/rat). Analyses of bronchoalveolar lavages showed early increases in the numbers of granulocytes, lymphocytes and multi-nucleated macrophages. The activation of macrophages was evaluated ex vivo by measurement of inflammatory mediator levels in culture supernatants. TNF-alpha and chemokine MCP-1, MIP-2 and CINC-1 production was elevated from 7 days after inhalation and remained so up to 3 months. In contrast, IL-1 beta, IL-6 and IL-10 production was unchanged. At 6 weeks, pulmonary macrophage numbers and activation state were increased as observed from an immunohistochemistry study of lung sections with anti-ED1. Similarly, histological analyses of lung sections also showed evidence of inflammatory responses. In conclusion, our results indicate early inflammatory changes in the lungs of PuO{sub 2}-contaminated animals and the involvement of macrophages in this process. A dose-effect relationship was observed between the amount of radionuclide inhaled or retained at the time of analysis and inflammatory mediator production by alveolar macrophages 14 days after exposure. For similar initial lung deposits, the inflammatory manifestation appears higher for 97% {sup 239}Pu than for 70% {sup 239}Pu. (authors)

  3. Involvement of ER stress and activation of apoptotic pathways in fisetin induced cytotoxicity in human melanoma.

    Science.gov (United States)

    Syed, Deeba N; Lall, Rahul K; Chamcheu, Jean Christopher; Haidar, Omar; Mukhtar, Hasan

    2014-12-01

    The prognosis of malignant melanoma remains poor in spite of recent advances in therapeutic strategies for the deadly disease. Fisetin, a dietary flavonoid is currently being investigated for its growth inhibitory properties in various cancer models. We previously showed that fisetin inhibited melanoma growth in vitro and in vivo. Here, we evaluated the molecular basis of fisetin induced cytotoxicity in metastatic human melanoma cells. Fisetin treatment induced endoplasmic reticulum (ER) stress in highly aggressive A375 and 451Lu human melanoma cells, as revealed by up-regulation of ER stress markers including IRE1α, XBP1s, ATF4 and GRP78. Time course analysis indicated that the ER stress was associated with activation of the extrinsic and intrinsic apoptotic pathways. Fisetin treated 2-D melanoma cultures displayed autophagic response concomitant with induction of apoptosis. Prolonged treatment (16days) with fisetin in a 3-D reconstituted melanoma model resulted in inhibition of melanoma progression with significant apoptosis, as evidenced by increased staining of cleaved Caspase-3 in the treated constructs. However, no difference in the expression of autophagic marker LC-3 was noted between treated and control groups. Fisetin treatment to 2-D melanoma cultures resulted in phosphorylation and activation of the multifunctional AMP-activated protein kinase (AMPK) involved in the regulation of diverse cellular processes, including autophagy and apoptosis. Silencing of AMPK failed to prevent cell death indicating that fisetin induced cytotoxicity is mediated through both AMPK-dependent and -independent mechanisms. Taken together, our studies confirm apoptosis as the primary mechanism through which fisetin inhibits melanoma cell growth and that activation of both extrinsic and intrinsic pathways contributes to fisetin induced cytotoxicity.

  4. The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity.

    Science.gov (United States)

    Gordillo, Miriam; Vega, Hugo; Trainer, Alison H; Hou, Fajian; Sakai, Norio; Luque, Ricardo; Kayserili, Hülya; Basaran, Seher; Skovby, Flemming; Hennekam, Raoul C M; Uzielli, Maria L Giovannucci; Schnur, Rhonda E; Manouvrier, Sylvie; Chang, Susan; Blair, Edward; Hurst, Jane A; Forzano, Francesca; Meins, Moritz; Simola, Kalle O J; Raas-Rothschild, Annick; Schultz, Roger A; McDaniel, Lisa D; Ozono, Keiichi; Inui, Koji; Zou, Hui; Jabs, Ethylin Wang

    2008-07-15

    Roberts syndrome/SC phocomelia (RBS) is an autosomal recessive disorder with growth retardation, craniofacial abnormalities and limb reduction. Cellular alterations in RBS include lack of cohesion at the heterochromatic regions around centromeres and the long arm of the Y chromosome, reduced growth capacity, and hypersensitivity to DNA damaging agents. RBS is caused by mutations in ESCO2, which encodes a protein belonging to the highly conserved Eco1/Ctf7 family of acetyltransferases that is involved in regulating sister chromatid cohesion. We identified 10 new mutations expanding the number to 26 known ESCO2 mutations. We observed that these mutations result in complete or partial loss of the acetyltransferase domain except for the only missense mutation that occurs in this domain (c.1615T>G, W539G). To investigate the mechanism underlying RBS, we analyzed ESCO2 mutations for their effect on enzymatic activity and cellular phenotype. We found that ESCO2 W539G results in loss of autoacetyltransferase activity. The cellular phenotype produced by this mutation causes cohesion defects, proliferation capacity reduction and mitomycin C sensitivity equivalent to those produced by frameshift and nonsense mutations associated with decreased levels of mRNA and absence of protein. We found decreased proliferation capacity in RBS cell lines associated with cell death, but not with increased cell cycle duration, which could be a factor in the development of phocomelia and cleft palate in RBS. In summary, we provide the first evidence that loss of acetyltransferase activity contributes to the pathogenesis of RBS, underscoring the essential role of the enzymatic activity of the Eco1p family of proteins.

  5. Antidepressant-like effect of creatine in mice involves dopaminergic activation.

    Science.gov (United States)

    Cunha, Mauricio P; Machado, Daniele G; Capra, Juliano C; Jacinto, Jardel; Bettio, Luis Eb; Rodrigues, Ana Lúcia S

    2012-11-01

    Creatine has been shown to play a significant role in health and disease. However, studies concerning its effect on mood are scarce. This study investigated the effect of creatine (p.o.) in the tail suspension test, a predictive test of antidepressant activity. Creatine reduced the immobility time in the tail suspension test (0.1-1000 mg/kg, male and female mice), without affecting locomotor activity. Furthermore, the involvement of the dopaminergic system in creatine-induced antidepressant-like effect in male mice in the tail suspension test was investigated. The anti-immobility effect of creatine (1 mg/kg) was prevented by the pre-treatment of mice with haloperidol (0.2 mg/kg, intraperitoneal (i.p.) route, non-selective dopamine receptor antagonist), (R)-(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride (SCH23390; 0.05 mg/kg, subcutaneous (s.c.) route, dopamine D₁ receptor antagonist) and sulpiride (50 mg/kg, i.p., dopamine D₂ receptor antagonist). Creatine (0.01 mg/kg, sub-effective dose) in combination with sub-effective doses of (1-phenyl-7,8-dihydroxy-2,3,4,5-tetrahydro-1H-3-benzazepine) hydrochloride (SKF38393; 0.1 mg/kg, s.c., dopamine D₁ receptor agonist), apomorphine (0.5 µg/kg, i.p., preferential dopamine D₂ receptor agonist) or bupropion (1 mg/kg, p.o., dopamine reuptake inhibitor with subtle activity on noradrenergic reuptake) reduced the immobility time in the tail suspension test as compared with either drug alone. These results indicate that the antidepressant-like effect of creatine is likely mediated by an activation of dopamine D₁ and D₂ receptors.

  6. Practice and Exploration on Multi-faceted Construction of Journalism Students Extracurricular Practice Platform%多方位构建新闻专业学生课外实践平台的实践与探索

    Institute of Scientific and Technical Information of China (English)

    陈臻; 龚洁宁

    2012-01-01

    围绕大学生创新精神和实践能力培养这一根本育人目标,以"倡导专业实践,提高学习兴趣"为基本出发点,以"多方面提高学生的媒介素养和社会责任感"为基本原则,以建构实践能力延展型课外路径为探索目标,在社团活动平台、文化素质平台、课外社会实践平台构建方面进行了一些实践与探索,取得了良好的效果。%Culture of the fundamental goals of educating people around the college students' innovative spirit and practical ability to construct a practical ability to "promote professional practice,and increase interest in learning the basic starting point" many ways to improve students' media literacy and social responsibility" as a basic principle,stretched extracurricular paths to explore the target platform of community activities,and cultural qualities of the platform,extracurricular social practice platform build some practice and exploration,and achieved good results.

  7. CaMKKβ is involved in AMP-activated protein kinase activation by baicalin in LKB1 deficient cell lines.

    Directory of Open Access Journals (Sweden)

    Ying Ma

    Full Text Available AMP-activated protein kinase (AMPK plays an important role in mediating energy metabolism and is controlled mainly by two upstream kinases, LKB1 or Ca(2+/calmodulin-dependent protein kinase kinase-β (CaMKKβ. Previously, we found that baicalin, one of the major flavonoids in a traditional Chinese herb medicine, Scutellaria baicalensis, protects against the development of hepatic steatosis in rats feeding with a high-fat diet by the activation of AMPK, but, the underlying mechanism for AMPK activation is unknown. Here we show that in two LKB1-deficient cells, HeLa and A549 cells, baicalin activates AMPK by α Thr-172 phosphorylation and subsequent phosphorylation of its downstream target, acetyl CoA carboxylase, at Ser-79, to a similar degree as does in HepG2 cells (that express LKB1. Pharmacologic inhibition of CaMKKβ by its selective inhibitor STO-609 markedly inhibits baicalin-induced AMPK activation in both HeLa and HepG2 cells, indicating that CaMKKβ is the responsible AMPK kinase. We also show that treatment of baicalin causes a larger increase in intracellular Ca(2+ concentration ([Ca(2+](i, although the maximal level of [Ca(2+](i is lower in HepG2 cells compared to HeLa cells. Chelation of intracellular free Ca(2+ by EDTA and EGTA, or depletion of intracellular Ca(2+ stores by the endoplasmic reticulum Ca(2+-ATPase inhibitor thapsigargin abrogates baicalin-induced activation of AMPK in HeLa cells. Neither cellular ATP nor the production of reactive oxygen species is altered by baicalin. Finally, in HeLa cells, baicalin treatment no longer decreases intracellular lipid accumulation caused by oleic acid after inhibition of CaMKKβ by STO-609. These results demonstrate that a potential Ca(2+/CaMKKβ dependent pathway is involved in the activation of AMPK by baicalin and suggest that CaMKKβ likely acts as an upstream kinase of AMPK in response to baicalin.

  8. Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases

    Directory of Open Access Journals (Sweden)

    Shaltiel Shmuel

    2003-07-01

    Full Text Available Abstract Background The plasminogen activator inhibitor-1 (PAI-1 spontaneously converts from an inhibitory into a latent form. Specificity of PAI-1 is mainly determined by its reactive site (Arg346-Met347, which interacts with serine residue of tissue-type plasminogen activator (tPA with concomitant formation of SDS-stable complex. Other sites may also play roles in determining the specificity of PAI-1 toward serine proteases. Results To understand more about the role of distal hinge for PAI-1 specificities towards serine proteases and for its conformational transition, wild type PAI-1 and its mutants were expressed in baculovirus system. WtPAI-1 was found to be about 12 fold more active than the fibrosarcoma PAI-1. Single site mutants within the Asp355-Arg356-Pro357 segment of PAI-1 yield guanidine activatable inhibitors (a that can still form SDS stable complexes with tPA and urokinase plasminogen activator (uPA, and (b that have inhibition rate constants towards plasminogen activators which resemble those of the fibrosarcoma inhibitor. More importantly, latency conversion rate of these mutants was found to be ~3–4 fold faster than that of wtPAI-1. We also tested if Glu351 is important for serine protease specificity. The functional stability of wtPAI-1, Glu351Ala, Glu351Arg was about 18 ± 5, 90 ± 8 and 14 ± 3 minutes, respectively, which correlated well with both their corresponding specific activities (84 ± 15 U/ug, 112 ± 18 U/ug and 68 ± 9 U/ug, respectively and amount of SDS-stable complex formed with tPA after denatured by Guanidine-HCl and dialyzed against 50 mM sodium acetate at 4°C. The second-order rate constants of inhibition for uPA, plasmin and thrombin by Glu351Ala and Glu351Arg were increased about 2–10 folds compared to wtPAI-1, but there was no change for tPA. Conclusion The Asp355-Pro357 segment and Glu351 in distal hinge are involved in maintaining the inhibitory conformation of PAI-1. Glu351 is a specificity

  9. Adolescents’ Sense of Community and Involvement in Playground Activities: Panacea to Ameliorate Social Vices and Delinquencies

    Directory of Open Access Journals (Sweden)

    Oluwagbemiga Paul Agboola

    2017-05-01

    Full Text Available Researchers have identified defects in the physical environments in which they interact and play, which has resulted in a decline the expected values initiated in both the social, physical and psychological developments. The role of playground in the development of adolescent’s health, moral and social standard has attracted lower interest in the recent time. The adolescent sense of community relates to a positive experience in the community open space setting such as playground and social well-being with their peers in general. Over time, little efforts have been initiated by the researchers towards these phenomena. This current study fills the gap by examining the adolescents’ sense of community through a quantitative survey via appraisal of the quality of community playground, emotional connection and effects of their participation in playground activities on ameliorating the delinquents’ behavior and social vices. Completed survey questionnaires retrieved from a total number of 69 purposive respondents who are adolescents from three towns and analyzed through relative importance index (RII via Likert scale. Results from the analysis indicated that adolescents’ positive attitudinal changes and reduction in social vices and delinquent’s behavior could be achieved through their involvements in quality and well-equipped playgrounds. Similarly, the significant role of sense of community in enhancing adolescent social participation in playground activities contributes to a major role in increasing their social well-being and togetherness. Thus, the study recommends appropriate future planning, design, and management of neighborhood playgrounds in Nigeria.

  10. Characterization of a specific arabinosyltransferase activity involved in mycobacterial arabinan biosynthesis.

    Science.gov (United States)

    Khasnobis, Shampa; Zhang, Jian; Angala, Shiva K; Amin, Anita G; McNeil, Michael R; Crick, Dean C; Chatterjee, Delphi

    2006-07-01

    Mycobacterium smegmatis strains that contain inactivated EmbA or EmbB proteins are unable to synthesize terminal Arabeta1-->2Araalpha1-->5(Arabeta1-->2Araalpha1-->3)Araalpha1-->5Araalpha1-->(Ara(6)) motif in the cell wall polysaccharide arabinogalactan. Instead, the mutants contain a linear Arabeta1-->2Araalpha1-->5Araalpha1-->5Araalpha1-->(Ara(4)) motif, suggesting that these proteins are involved in the synthesis or transfer of the disaccharide Arabeta1-->2Araalpha1--> to an internal 5-linked Ara. Therefore, we synthesized a linear Arabeta1-->2Araalpha1-->5Araalpha1-->5Araalpha1-->5Araalpha1--> with an octyl aglycon as an arabinosyl acceptor in cell-free assays. A facile assay was developed using the chemically synthesized glycan, membrane, and particulate cell wall as the enzyme source, and 5-phosphoribose diphosphate pR[(14)C]pp as the arabinose donor. The results unequivocally show that two arabinofuranosyl residues were added at the tertiary -->5Araalpha1--> of the synthetic glycan. This activity was undetectable in strains of M. smegmatis where embB or embA had been genetically disrupted. Normal activity could be restored only in the presence of both EmbA and EmbB proteins.

  11. Involvement of JNK and Caspase Activation in Hoiamide A-Induced Neurotoxicity in Neocortical Neurons

    Directory of Open Access Journals (Sweden)

    Zhengyu Cao

    2015-02-01

    Full Text Available The frequent occurrence of Moorea producens (formerly Lyngbya majuscula blooms has been associated with adverse effects on human health. Hoiamide A is a structurally unique cyclic depsipeptide isolated from an assemblage of the marine cyanobacteria M. producens and Phormidium gracile. We examined the influence of hoiamide A on neurite outgrowth in neocortical neurons and found that it suppressed neurite outgrowth with an IC50 value of 4.89 nM. Further study demonstrated that hoiamide A stimulated lactic acid dehydrogenase (LDH efflux, nuclear condensation and caspase-3 activity with EC50 values of 3.66, 2.55 and 4.33 nM, respectively. These data indicated that hoiamide A triggered a unique neuronal death profile that involves both necrotic and apoptotic mechanisms. The similar potencies and similar time-response relationships between LDH efflux and caspase-3 activation/nuclear condensation suggested that both necrosis and apoptosis may derive from interaction with a common molecular target. The broad-spectrum caspase inhibitor, Z-VAD-FMK completely inhibited hoiamide A-induced neurotoxicity. Additionally, hoiamide A stimulated JNK phosphorylation, and a JNK inhibitor attenuated hoiamide A-induced neurotoxicity. Collectively, these data demonstrate that hoiamide A-induced neuronal death requires both JNK and caspase signaling pathways. The potent neurotoxicity and unique neuronal cell death profile of hoiamide A represents a novel neurotoxic chemotype from marine cyanobacteria.

  12. Nisin and lysostaphin activity against preformed biofilm of Staphylococcus aureus involved in bovine mastitis.

    Science.gov (United States)

    Ceotto-Vigoder, H; Marques, S L S; Santos, I N S; Alves, M D B; Barrias, E S; Potter, A; Alviano, D S; Bastos, M C F

    2016-07-01

    The biofilm produced by Staphylococcus aureus isolates involved in clinical or subclinical bovine mastitis and the activity of nisin and lysostaphin against the preformed biofilm produced by these strains were investigated. Eighteen strains were tested and all produced biofilm. Eight strains with distinct biofilm composition were selected for the antimicrobial activity assays. The minimal inhibitory concentration of each bacteriocin was determined against the planktonic cells and ranged from 15·6 to 500 μg ml(-1) for nisin, and from 3·9 to 50 μg ml(-1) , for lysostaphin. Lysostaphin treatment (0·4 μg ml(-1) ) for 4 h caused a strong Staph. aureus 4181 biofilm detachment and death of the majority of the sessile cells, while nisin treatment (100 μg ml(-1) ) for the same time caused only a great reduction in cell viability. Additionally, combination of both bacteriocins for 4 h resulted in significant death of the sessile cells but no biofilm detachment. The treatment with lysostaphin alone or in combination with nisin was effective in killing most biofilm sessile cells. The action of lysostaphin, either alone or in combination with nisin, against established staphylococcal biofilm may represent an alternative to bovine mastitis control. However, the duration of the treatment should be considered for its application so that the best effectiveness can be achieved. © 2016 The Society for Applied Microbiology.

  13. Association of father involvement and neighborhood quality with kindergartners' physical activity: a multilevel structural equation model.

    Science.gov (United States)

    Beets, Michael W; Foley, John T

    2008-01-01

    Examine the effects of father-child involvement and neighborhood characteristics with young children's physical activity (PA) within a multilevel framework. Cross-sectional analysis of the Early Childhood Longitudinal Study-Kindergarten Cohort 1998. Nationally representative sample. Data were available for 10,694 kindergartners (5-6 years; 5240 girls) living in 1053 neighborhoods. Parental report of child's PA level, father characteristics (e.g., time spent with child, age, education, socioeconomic status, hours worked), family time spent doing sports/ activities together, and neighborhood quality (e.g., safety, presence of crime violence, garbage). Child weight status, motor skills, ethnicity, and television viewing were used as covariates. Multilevel structural equation modeling with children nested within neighborhoods. At the child level father-child time and family time doing sports together were positively associated with children's PA. At the neighborhood level parental perception of a neighborhood's safety for children to play outside fully mediated the effect of neighborhood quality on children's PA. Overall 19.1% and 7.6% of the variance in PA was explained at the child and neighborhood levels, respectively. Family-based interventions for PA should consider father-child time, with this contributing to a child's overall PA level. Further, neighborhood quality is an important predictor of PA only to the extent by which parents perceive it to be unsafe for their child to play outdoors.

  14. Mastitis and fertility in cattle - possible involvement of inflammation or immune activation in embryonic mortality.

    Science.gov (United States)

    Hansen, Peter J; Soto, Paolete; Natzke, Roger P

    2004-04-01

    Causes for pre-implantation embryo loss, which can be as high as 50% or more of fertilized embryos, are multifactorial and largely undescribed. Studies in cattle using mastitis as a model indicate that one cause of early embryonic loss is infectious disease or activation of immune responses at sites outside the reproductive tract. Infection of the mammary gland in dairy cattle is associated with a reduction in pregnancy rate (proportion of inseminated cows that become pregnant) and an increase in the number of inseminations required to establish pregnancy. Also, intravenous challenge with bacterial peptidoglycan and polysaccharide at approximately days 3-5 after breeding reduced subsequent pregnancy rate in sheep that had been previously immunized against the same material. The mechanism by which extrauterine activation of immune and inflammatory responses leads to embryonic loss is not clear although cytokines probably play a crucial role. Effects could be exerted at the level of the hypothalamic-pituitary axis, ovary, reproductive tract or embryo. Interferon (IFN)-alpha, for example, which can reduce pregnancy rate in cattle when injected around 13-19 days after breeding, increases body temperature, inhibits secretion of luteinizing hormone, and reduces circulating concentrations of progesterone. Other cytokines or products of cytokine activation could cause embryonic loss by causing hyperthermia (as elevated temperature blocks oocyte function and embryonic development), exerting toxic effects on the corpus luteum [for example, IFN-gamma, tumor necrosis factor-alpha (TNF-alpha) and prostaglandin F(2alpha)], stimulating endometrial prostaglandin synthesis [TNF-alpha and interleukin(IL)-1beta], reducing endometrial cell proliferation (IL-1beta), and interfering with oocyte maturation and embryonic development (TNF-alpha, nitric oxide, and prostaglandin F(2alpha)). Although largely neglected by reproductive immunologists, study of the involvement of the immune

  15. Modulation of NCC activity by low and high K+ intake: insights into the signaling pathways involved

    Science.gov (United States)

    Castañeda-Bueno, María; Cervantes-Perez, Luz Graciela; Rojas-Vega, Lorena; Arroyo-Garza, Isidora; Vázquez, Norma; Moreno, Erika

    2014-01-01

    Modulation of Na+-Cl− cotransporter (NCC) activity is essential to adjust K+ excretion in the face of changes in dietary K+ intake. We used previously characterized genetic mouse models to assess the role of Ste20-related proline-alanine-rich kinase (SPAK) and with-no-lysine kinase (WNK)4 in the modulation of NCC by K+ diets. SPAK knockin and WNK4 knockout mice were placed on normal-, low-, or high-K+-citrate diets for 4 days. The low-K+ diet decreased and high-K+ diet increased plasma aldosterone levels, but both diets were associated with increased phosphorylation of NCC (phospho-NCC, Thr44/Thr48/Thr53) and phosphorylation of SPAK/oxidative stress responsive kinase 1 (phospho-SPAK/OSR1, Ser383/Ser325). The effect of the low-K+ diet on SPAK phosphorylation persisted in WNK4 knockout and SPAK knockin mice, whereas the effects of ANG II on NCC and SPAK were lost in both mouse colonies. This suggests that for NCC activation by ANG II, integrity of the WNK4/SPAK pathway is required, whereas for the low-K+ diet, SPAK phosphorylation occurred despite the absence of WNK4, suggesting the involvement of another WNK (WNK1 or WNK3). Additionally, because NCC activation also occurred in SPAK knockin mice, it is possible that loss of SPAK was compensated by OSR1. The positive effect of the high-K+ diet was observed when the accompanying anion was citrate, whereas the high-KCl diet reduced NCC phosphorylation. However, the effect of the high-K+-citrate diet was aldosterone dependent, and neither metabolic alkalosis induced by bicarbonate, nor citrate administration in the absence of K+ increased NCC phosphorylation, suggesting that it was not due to citrate-induced metabolic alkalosis. Thus, the accompanying anion might modulate the NCC response to the high-K+ diet. PMID:24761002

  16. Modulation of NCC activity by low and high K(+) intake: insights into the signaling pathways involved.

    Science.gov (United States)

    Castañeda-Bueno, María; Cervantes-Perez, Luz Graciela; Rojas-Vega, Lorena; Arroyo-Garza, Isidora; Vázquez, Norma; Moreno, Erika; Gamba, Gerardo

    2014-06-15

    Modulation of Na(+)-Cl(-) cotransporter (NCC) activity is essential to adjust K(+) excretion in the face of changes in dietary K(+) intake. We used previously characterized genetic mouse models to assess the role of Ste20-related proline-alanine-rich kinase (SPAK) and with-no-lysine kinase (WNK)4 in the modulation of NCC by K(+) diets. SPAK knockin and WNK4 knockout mice were placed on normal-, low-, or high-K(+)-citrate diets for 4 days. The low-K(+) diet decreased and high-K(+) diet increased plasma aldosterone levels, but both diets were associated with increased phosphorylation of NCC (phospho-NCC, Thr(44)/Thr(48)/Thr(53)) and phosphorylation of SPAK/oxidative stress responsive kinase 1 (phospho-SPAK/OSR1, Ser(383)/Ser(325)). The effect of the low-K(+) diet on SPAK phosphorylation persisted in WNK4 knockout and SPAK knockin mice, whereas the effects of ANG II on NCC and SPAK were lost in both mouse colonies. This suggests that for NCC activation by ANG II, integrity of the WNK4/SPAK pathway is required, whereas for the low-K(+) diet, SPAK phosphorylation occurred despite the absence of WNK4, suggesting the involvement of another WNK (WNK1 or WNK3). Additionally, because NCC activation also occurred in SPAK knockin mice, it is possible that loss of SPAK was compensated by OSR1. The positive effect of the high-K(+) diet was observed when the accompanying anion was citrate, whereas the high-KCl diet reduced NCC phosphorylation. However, the effect of the high-K(+)-citrate diet was aldosterone dependent, and neither metabolic alkalosis induced by bicarbonate, nor citrate administration in the absence of K(+) increased NCC phosphorylation, suggesting that it was not due to citrate-induced metabolic alkalosis. Thus, the accompanying anion might modulate the NCC response to the high-K(+) diet. Copyright © 2014 the American Physiological Society.

  17. Do Public Involvement Activities in Biomedical Research and Innovation Recruit Representatively? A Systematic Qualitative Review.

    Science.gov (United States)

    Lander, Jonas; Hainz, Tobias; Hirschberg, Irene; Bossert, Sabine; Strech, Daniel

    2016-01-01

    Public involvement activities (PIAs) may contribute to the governance of ethically challenging biomedical research and innovation by informing, consulting with and engaging the public in developments and decision-making processes. For PIAs to capture a population's preferences (e.g. on issues in whole genome sequencing, biobanks or genome editing), a central methodological requirement is to involve a sufficiently representative subgroup of the general public. While the existing literature focusses on theoretical and normative aspects of 'representation', this study assesses empirically how such considerations are implemented in practice. It evaluates how PIA reports describe representation objectives, the recruitment process and levels of representation achieved. PIA reports were included from a systematic literature search if they directly reported a PIA conducted in a relevant discipline such as genomics, biobanks, biotechnology or others. PIA reports were analyzed with thematic text analysis. The text analysis was guided by an assessment matrix based on PIA-specific guidelines and frameworks. We included 46 relevant reports, most focusing on issues in genomics. 27 reports (59%) explicitly described representation objectives, though mostly without adjusting eligibility criteria and recruiting methods to the specific objective. 11 reports (24%) explicitly reported to have achieved the intended representation; the rest either reported failure or were silent on this issue. Representation of study samples in PIAs in biomedical research and innovation is currently not reported systematically. Improved reporting on representation would not only improve the validity and value of PIAs, but could also contribute to PIA results being used more often in relevant policy and decision-making processes. © 2016 S. Karger AG, Basel.

  18. Mothers' and Fathers' Involvement in Home Activities with Their Children: Psychosocial Factors and the Role of Parental Self-Efficacy

    Science.gov (United States)

    Giallo, Rebecca; Treyvaud, Karli; Cooklin, Amanda; Wade, Catherine

    2013-01-01

    Parent involvement in play, learning, and everyday home activities is important for promoting children's cognitive and language development. The aims of the study were to (a) examine differences between mothers' and fathers' self-reported involvement with their children, (b) explore the relationship between child, parent and family factors, and…

  19. A mass spectrometric method to determine activities of enzymes involved in polyamine catabolism

    Energy Technology Data Exchange (ETDEWEB)

    Moriya, Shunsuke; Iwasaki, Kaori [Department of Molecular Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-kitazawa, Setagaya-ku, Tokyo 156-8506 (Japan); Samejima, Keijiro, E-mail: samejima-kj@igakuken.or.jp [Department of Molecular Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-kitazawa, Setagaya-ku, Tokyo 156-8506 (Japan); Takao, Koichi [Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295 (Japan); Kohda, Kohfuku [Research Institute of Pharmaceutical Sciences, Musashino University, 1-1-20 Shinmachi, Nishitokyo, Tokyo 202-8585 (Japan); Hiramatsu, Kyoko; Kawakita, Masao [Department of Molecular Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-kitazawa, Setagaya-ku, Tokyo 156-8506 (Japan)

    2012-10-20

    Highlights: Black-Right-Pointing-Pointer Compounds in polyamine catabolic pathway were determined by a column-free ESI-TOF MS. Black-Right-Pointing-Pointer N{sup 1}- and N{sup 8}-acetylspermidine were determined by a column-free ESI-MS/MS. Black-Right-Pointing-Pointer The method was applied to determine activities of APAO, SMO, and SSAT in the pathway. Black-Right-Pointing-Pointer The assay method contained stable isotope-labeled natural substrates. Black-Right-Pointing-Pointer It is applicable to biological samples containing natural substrate and product. - Abstract: An analytical method for the determination of three polyamines (putrescine, spermidine, and spermine) and five acetylpolyamines [N{sup 1}-acetylspermidine (N{sup 1}AcSpd), N{sup 8}-acetylspermidine (N{sup 8}AcSpd), N{sup 1}-acetylspermine, N{sup 1},N{sup 8}-diacetylspermidine, and N{sup 1},N{sup 12}-diacetylspermine] involved in the polyamine catabolic pathway has been developed using a hybrid tandem mass spectrometer. Heptafluorobutyryl (HFB) derivatives of these compounds and respective internal standards labeled with stable isotopes were analyzed simultaneously by TOF MS, based on peak areas appearing at appropriate m/z values. The isomers, N{sup 1}AcSpd and N{sup 8}AcSpd were determined from their fragment ions, the acetylamidopropyl and acetylamidobutyl groups, respectively, using MS/MS with {sup 13}C{sub 2}-N{sup 1}AcSpd and {sup 13}C{sub 2}-N{sup 8}AcSpd which have the {sup 13}C{sub 2}-acetyl group as an internal standard. The TOF MS method was successfully applied to measure the activity of enzymes involved in polyamine catabolic pathways, namely N{sup 1}-acetylpolyamine oxidase (APAO), spermine oxidase (SMO), and spermidine/spermine N{sup 1}-acetyltransferase (SSAT). The following natural substrates and products labeled with stable isotopes considering the application to biological samples were identified; for APAO, [4,9,12-{sup 15}N{sub 3}]-N{sup 1}-acetylspermine and [1,4,8-{sup 15}N{sub 3

  20. Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement

    KAUST Repository

    He, Li-Sheng

    2012-10-24

    The barnacle Balanus ( = Amphibalanus) amphitrite is a major marine fouling animal. Understanding the molecular mechanism of larval settlement in this species is critical for anti-fouling research. In this study, we cloned one isoform of p38 MAPK (Bar-p38 MAPK) from this species, which shares the significant characteristic of containing a TGY motif with other species such as yeast, Drosophila and humans. The activation of p38 MAPK was detected by an antibody that recognizes the conserved dual phosphorylation sites of TGY. The results showed that phospho-p38 MAPK (pp38 MAPK) was more highly expressed at the cyprid stage, particularly in aged cyprids, in comparison to other stages, including the nauplius and juvenile stages. Immunostaining showed that Bar-p38 MAPK and pp38 MAPK were mainly located at the cyprid antennules, and especially the third and fourth segments, which are responsible for substratum exploration during settlement. The expression and localization patterns of Bar-p38 MAPK suggest its involvement in larval settlement. This postulation was also supported by the larval settlement bioassay with the p38 MAPK inhibitor SB203580. Behavioral analysis by live imaging revealed that the larvae were still capable of exploring the surface of the substratum after SB203580 treatment. This shows that the effect of p38 MAPK on larval settlement might be by regulating the secretion of permanent proteinaceous substances. Furthermore, the level of pp38 MAPK dramatically decreased after full settlement, suggesting that Bar-p38 MAPK maybe plays a role in larval settlement rather than metamorphosis. Finally, we found that Bar-p38 MAPK was highly activated when larvae confronted extracts of adult barnacle containing settlement cues, whereas larvae pre-treated with SB203580 failed to respond to the crude adult extracts.

  1. PRESS40: a project for involving students in active seismic risk mitigation

    Science.gov (United States)

    Barnaba, Carla; Contessi, Elisa; Rosa Girardi, Maria

    2016-04-01

    To memorialize the anniversary of the 1976 Friuli earthquake, the Istituto Statale di Istruzione Superiore "Magrini Marchetti" in Gemona del Friuli (NE Italy), with the collaboration of the Istituto Nazionale di Oceanografia e di Geofisica Sperimentale (OGS), has promoted the PRESS40 Project (Prevenzione Sismica nella Scuola a 40 anni dal terremoto del Friuli, that in English sounds like "Seismic Prevention at School 40 years later the Friuli earthquake"). The project has developed in the 2015-2016 school year, starting from the 40th anniversary of the Friuli earthquake, and it aims to disseminate historical memory, seismic culture and awareness of seismic safety in the young generations, too often unconscious of past experiences, as recent seismic hazard perception tests have demonstrated. The basic idea of the PRESS40 Project is to involve the students in experimental activities to be active part of the seismic mitigation process. The Project is divided into two main parts, the first one in which students learn-receive knowledge from researchers, and the second one in which they teach-bring knowledge to younger students. In the first part of the project, 75 students of the "Magrini Marchetti" school acquired new geophysical data, covering the 23 municipalities from which they come from. These municipalities represent a wide area affected by the 1976 Friuli earthquake. In each locality a significant site was examined, represented by a school area. At least, 127 measurements of ambient noise have been acquired. Data processing and interpretation of all the results are still going on, under the supervision of OGS researchers.The second part of the project is planned for the early spring, when the students will present the results of geophysical survey to the younger ones of the monitored schools and to the citizens in occasion of events to commemorate the 40th anniversary of the Friuli earthquake.

  2. Evidence for the involvement of p38 MAPK activation in barnacle larval settlement.

    Directory of Open Access Journals (Sweden)

    Li-Sheng He

    Full Text Available The barnacle Balanus ( = Amphibalanus amphitrite is a major marine fouling animal. Understanding the molecular mechanism of larval settlement in this species is critical for anti-fouling research. In this study, we cloned one isoform of p38 MAPK (Bar-p38 MAPK from this species, which shares the significant characteristic of containing a TGY motif with other species such as yeast, Drosophila and humans. The activation of p38 MAPK was detected by an antibody that recognizes the conserved dual phosphorylation sites of TGY. The results showed that phospho-p38 MAPK (pp38 MAPK was more highly expressed at the cyprid stage, particularly in aged cyprids, in comparison to other stages, including the nauplius and juvenile stages. Immunostaining showed that Bar-p38 MAPK and pp38 MAPK were mainly located at the cyprid antennules, and especially the third and fourth segments, which are responsible for substratum exploration during settlement. The expression and localization patterns of Bar-p38 MAPK suggest its involvement in larval settlement. This postulation was also supported by the larval settlement bioassay with the p38 MAPK inhibitor SB203580. Behavioral analysis by live imaging revealed that the larvae were still capable of exploring the surface of the substratum after SB203580 treatment. This shows that the effect of p38 MAPK on larval settlement might be by regulating the secretion of permanent proteinaceous substances. Furthermore, the level of pp38 MAPK dramatically decreased after full settlement, suggesting that Bar-p38 MAPK maybe plays a role in larval settlement rather than metamorphosis. Finally, we found that Bar-p38 MAPK was highly activated when larvae confronted extracts of adult barnacle containing settlement cues, whereas larvae pre-treated with SB203580 failed to respond to the crude adult extracts.

  3. Deregulated Cdk5 Activity Is Involved in Inducing Alzheimer’s Disease

    Science.gov (United States)

    Shukla, Varsha; Skuntz, Susan; Pant, Harish C.

    2012-01-01

    Alzheimer’s disease (AD), the most devastating chronic neurodegenerative disease in adults, causes dementia and eventually, death of the affected individuals. Clinically, AD is characterized as late-onset, age-dependent cognitive decline due to loss of neurons in cortex and hippocampus. The pathologic corollary of these symptoms is the formation of senile plaques and neurofibrillary tangles. Senile plaques are formed due to accumulation of oligomeric amyloid beta (Aβ) forming fibrillary plaques. This occurs due to the amyloidogenic processing of the amyloid precursor protein (APP) by various secretases. On the other hand, neurofibrillary tangles are formed due to hyperphosphorylation of cytoskeleton proteins like tau and neurofilament. Both are hyperphosphorylated by cyclin-dependent kinase-5 (Cdk5) and are part of the paired helical filament (PHF), an integral part of neurofibrillary tangles. Unlike other cyclin-dependent kinases, Cdk5 plays a very important role in the neuronal development. Cdk5 gets activated by its neuronal activators p35 and p39. Upon stress, p35 and p39 are cleaved by calpain resulting in truncated products as p25 and p29. Association of Cdk5/p25 is longer and uncontrolled causing aberrant hyperphosphorylation of various substrates of Cdk5 like APP, tau and neurofilament, leading to neurodegenerative pathology like AD. Additionally recent evidence has shown increased levels of p25, Aβ, hyperactivity of Cdk5, phosphorylated tau and neurofilament in human AD brains. This review briefly describes the above-mentioned aspects of involvement of Cdk5 in the pathology of AD and at the end summarizes the advances in Cdk5 as a therapeutic target. PMID:23142263

  4. Cocaine-mediated microglial activation involves the ER stress-autophagy axis.

    Science.gov (United States)

    Guo, Ming-Lei; Liao, Ke; Periyasamy, Palsamy; Yang, Lu; Cai, Yu; Callen, Shannon E; Buch, Shilpa

    2015-01-01

    Cocaine abuse leads to neuroinflammation, which, in turn, contributes to the pathogenesis of neurodegeneration associated with advanced HIV-1 infection. Autophagy plays important roles in both innate and adaptive immune responses. However, the possible functional link between cocaine and autophagy has not been explored before. Herein, we demonstrate that cocaine exposure induced autophagy in both BV-2 and primary rat microglial cells as demonstrated by a dose- and time-dependent induction of autophagy-signature proteins such as BECN1/Beclin 1, ATG5, and MAP1LC3B. These findings were validated wherein cocaine treatment of BV-2 cells resulted in increased formation of puncta in cells expressing either endogenous MAP1LC3B or overexpressing GFP-MAP1LC3B. Specificity of cocaine-induced autophagy was confirmed by treating cells with inhibitors of autophagy (3-MA and wortmannin). Intriguingly, cocaine-mediated induction of autophagy involved upstream activation of 2 ER stress pathways (EIF2AK3- and ERN1-dependent), as evidenced by the ability of the ER stress inhibitor salubrinal to ameliorate cocaine-induced autophagy. In vivo validation of these findings demonstrated increased expression of BECN1, ATG5, and MAP1LC3B-II proteins in cocaine-treated mouse brains compared to untreated animals. Increased autophagy contributes to cocaine-mediated activation of microglia since pretreatment of cells with wortmannin resulted in decreased expression and release of inflammatory factors (TNF, IL1B, IL6, and CCL2) in microglial cells. Taken together, our findings suggest that cocaine exposure results in induction of autophagy that is closely linked with neuroinflammation. Targeting autophagic proteins could thus be considered as a therapeutic strategy for the treatment of cocaine-related neuroinflammation diseases.

  5. Activation of Holliday junction recognizing protein involved in the chromosomal stability and immortality of cancer cells.

    Science.gov (United States)

    Kato, Tatsuya; Sato, Nagato; Hayama, Satoshi; Yamabuki, Takumi; Ito, Tomoo; Miyamoto, Masaki; Kondo, Satoshi; Nakamura, Yusuke; Daigo, Yataro

    2007-09-15

    We identified a novel gene HJURP (Holliday junction-recognizing protein) whose activation seemed to play a pivotal role in the immortality of cancer cells. HJURP was considered a possible downstream target for ataxia telangiectasia mutated signaling, and its expression was increased by DNA double-strand breaks (DSB). HJURP was involved in the homologous recombination pathway in the DSB repair process through interaction with hMSH5 and NBS1, which is a part of the MRN protein complex. HJURP formed nuclear foci in cells at S phase and those subjected to DNA damage. In vitro assays implied that HJURP bound directly to the Holliday junction and rDNA arrays. Treatment of cancer cells with small interfering RNA (siRNA) against HJURP caused abnormal chromosomal fusions and led to genomic instability and senescence. In addition, HJURP overexpression was observed in a majority of lung cancers and was associated with poor prognosis as well. We suggest that HJURP is an indispensable factor for chromosomal stability in immortalized cancer cells and is a potential novel therapeutic target for the development of anticancer drugs.

  6. Water-soluble chlorophyll protein is involved in herbivore resistance activation during greening of Arabidopsis thaliana

    Science.gov (United States)

    Boex-Fontvieille, Edouard; Rustgi, Sachin; von Wettstein, Diter; Reinbothe, Steffen; Reinbothe, Christiane

    2015-01-01

    Water-soluble chlorophyll proteins (WSCPs) constitute a small family of unusual chlorophyll (Chl)-binding proteins that possess a Kunitz-type protease inhibitor domain. In Arabidopsis thaliana, a WSCP has been identified, named AtWSCP, that forms complexes with Chl and the Chl precursor chlorophyllide (Chlide) in vitro. AtWSCP exhibits a quite unexpected expression pattern for a Chl binding protein and accumulated to high levels in the apical hook of etiolated plants. AtWSCP expression was negatively light-regulated. Transgenic expression of AtWSCP fused to green fluorescent protein (GFP) revealed that AtWSCP is localized to cell walls/apoplastic spaces. Biochemical assays identified AtWSCP as interacting with RD21 (RESPONSIVE TO DESICCATION 21), a granulin domain-containing cysteine protease implicated in stress responses and defense. Reconstitution experiments showed tight interactions between RD21 and WSCP that were relieved upon Chlide binding. Laboratory feeding experiments with two herbivorous isopod crustaceans, Porcellio scaber (woodlouse) and Armadillidium vulgare (pillbug), identified the apical hook as Achilles’ heel of etiolated plants and that this was protected by RD21 during greening. Because Chlide is formed in the apical hook during seedling emergence from the soil, our data suggest an unprecedented mechanism of herbivore resistance activation that is triggered by light and involves AtWSCP. PMID:26016527

  7. Residual NADPH Oxidase Activity and Isolated Lung Involvement in X-Linked Chronic Granulomatous Disease

    Directory of Open Access Journals (Sweden)

    Maria J. Gutierrez

    2012-01-01

    Full Text Available Chronic granulomatous disease (CGD is characterized by inherited immune defects resulting from mutations in the NADPH oxidase complex genes. The X-linked type of CGD is caused by defects in the CYBB gene that encodes gp91-phox, a fundamental component of the NADPH oxidase complex. This mutation originates the most common and severe form of CGD, which typically has absence of NADPH oxidase function and aggressive multisystemic infections. We present the case of a 9-year-old child with a rare CYBB mutation that preserves some NADPH oxidase activity, resulting in an atypical mild form of X-linked CGD with isolated lung involvement. Although the clinical picture and partially preserved oxidase function suggested an autosomal recessive form of CGD, genetic testing demonstrated a mutation in the exon 3 of CYBB gene (c.252 G>A, p.Ala84Ala, an uncommon X-linked CGD variant that affects splicing. Atypical presentation and diagnostic difficulties are discussed. This case highlights that the diagnosis of mild forms of X-linked CGD caused by rare CYBB mutations and partially preserved NADPH function should be considered early in the evaluation of atypical and recurrent lung infections.

  8. Current practice of public involvement activities in biomedical research and innovation: a systematic qualitative review.

    Directory of Open Access Journals (Sweden)

    Jonas Lander

    Full Text Available BACKGROUND: A recent report from the British Nuffield Council on Bioethics associated 'emerging biotechnologies' with a threefold challenge: 1 uncertainty about outcomes, 2 diverse public views on the values and implications attached to biotechnologies and 3 the possibility of creating radical changes regarding societal relations and practices. To address these challenges, leading international institutions stress the need for public involvement activities (PIAs. The objective of this study was to assess the state of PIA reports in the field of biomedical research. METHODS: PIA reports were identified via a systematic literature search. Thematic text analysis was employed for data extraction. RESULTS: After filtering, 35 public consultation and 11 public participation studies were included in this review. Analysis and synthesis of all 46 PIA studies resulted in 6 distinguishable PIA objectives and 37 corresponding PIA methods. Reports of outcome translation and PIA evaluation were found in 9 and 10 studies respectively (20% and 22%. The paper presents qualitative details. DISCUSSION: The state of PIAs on biomedical research and innovation is characterized by a broad range of methods and awkward variation in the wording of objectives. Better comparability of PIAs might improve the translation of PIA findings into further policy development. PIA-specific reporting guidelines would help in this regard. The modest level of translation efforts is another pointer to the "deliberation to policy gap". The results of this review could inform the design of new PIAs and future efforts to improve PIA comparability and outcome translation.

  9. Exit of Plasmodium sporozoites from oocysts is an active process that involves the circumsporozoite protein.

    Directory of Open Access Journals (Sweden)

    2005-09-01

    Full Text Available Plasmodium sporozoites develop within oocysts residing in the mosquito midgut. Mature sporozoites exit the oocysts, enter the hemolymph, and invade the salivary glands. The circumsporozoite (CS protein is the major surface protein of salivary gland and oocyst sporozoites. It is also found on the oocyst plasma membrane and on the inner surface of the oocyst capsule. CS protein contains a conserved motif of positively charged amino acids: region II-plus, which has been implicated in the initial stages of sporozoite invasion of hepatocytes. We investigated the function of region II-plus by generating mutant parasites in which the region had been substituted with alanines. Mutant parasites produced normal numbers of sporozoites in the oocysts, but the sporozoites were unable to exit the oocysts. In in vitro as well, there was a profound delay, upon trypsin treatment, in the release of mutant sporozoites from oocysts. We conclude that the exit of sporozoites from oocysts is an active process that involves the region II-plus of CS protein. In addition, the mutant sporozoites were not infective to young rats. These findings provide a new target for developing reagents that interfere with the transmission of malaria.

  10. Postnatal changes of local neuronal circuits involved in activation of jaw-closing muscles.

    Science.gov (United States)

    Inoue, Tomio; Nakamura, Shiro; Takamatsu, Junichi; Tokita, Kenichi; Gemba, Akiko; Nakayama, Kiyomi

    2007-04-01

    Feeding behaviour in mammals changes from suckling to mastication during postnatal development and the neuronal circuits controlling feeding behaviour should change in parallel to the development of orofacial structures. In this review we discuss the location of excitatory premotor neurons for jaw-closing motoneurons (JCMNs) and postnatal changes of excitatory synaptic transmission from the supratrigeminal region (SupV) to JCMNs. We show that neurons located in SupV and the reticular formation dorsal to the facial nucleus most likely excite JCMNs. Excitatory inputs from SupV to JCMNs are mediated by activation of glutamate and glycine receptors in neonatal rats, whereas glycinergic inputs from SupV to JCMNs become inhibitory with age. We also show that the incidence of post-spike afterdepolarization increases during postnatal development, whereas the amplitude and half-duration of the medium-duration afterhyperpolarization decrease with age. Such postnatal changes in synaptic transmission from SupV to JCMNs and membrane properties of JCMNs might be involved in the transition from suckling to mastication.

  11. Current practice of public involvement activities in biomedical research and innovation: a systematic qualitative review.

    Science.gov (United States)

    Lander, Jonas; Hainz, Tobias; Hirschberg, Irene; Strech, Daniel

    2014-01-01

    A recent report from the British Nuffield Council on Bioethics associated 'emerging biotechnologies' with a threefold challenge: 1) uncertainty about outcomes, 2) diverse public views on the values and implications attached to biotechnologies and 3) the possibility of creating radical changes regarding societal relations and practices. To address these challenges, leading international institutions stress the need for public involvement activities (PIAs). The objective of this study was to assess the state of PIA reports in the field of biomedical research. PIA reports were identified via a systematic literature search. Thematic text analysis was employed for data extraction. After filtering, 35 public consultation and 11 public participation studies were included in this review. Analysis and synthesis of all 46 PIA studies resulted in 6 distinguishable PIA objectives and 37 corresponding PIA methods. Reports of outcome translation and PIA evaluation were found in 9 and 10 studies respectively (20% and 22%). The paper presents qualitative details. The state of PIAs on biomedical research and innovation is characterized by a broad range of methods and awkward variation in the wording of objectives. Better comparability of PIAs might improve the translation of PIA findings into further policy development. PIA-specific reporting guidelines would help in this regard. The modest level of translation efforts is another pointer to the "deliberation to policy gap". The results of this review could inform the design of new PIAs and future efforts to improve PIA comparability and outcome translation.

  12. La inclusió de l’alumnat nouvingut mitjançant la pràctica d’activitats físicoesportives extraescolars The inclusion of immigrant students through the practice of extracurricular physico-sports activities La inclusión del alumnado inmigrante mediante la práctica de actividades físico-deportivas extraescolares

    Directory of Open Access Journals (Sweden)

    Miguel Ángel Torralba i Jordan

    2007-01-01

    Full Text Available Una de les conseqüències directes del fenomen de la diversitat cultural és l’elevat nombre d’alumnat nouvingut als centres educatius. Davant d’aquest fet, les activitats físicoesportives poden esdevenir una bona estratègia per afrontar amb eficàcia el repte d’assolir una educació inclusiva i per a tothom. Aquest article presenta un estudi dut a terme als centres educatius reconeguts oficialment com a centres amb aula d’acollida. Concretament, mostra els resultats obtinguts en l’aplicació d’un qüestionari complimentat pels educadors (professorat, mestres i altres agents educatius amb la intenció de copsar el valor inclusiu de les activitats físicoesportives dutes a terme en horari no-lectiu. _________________________________________________________________________________________________________________________________________________________________________________________ L’une des conséquences directes du phénomène de la diversité culturelle est le grand nombre d’élèves récemment arrivés dans les centres éducatifs. Face à ce fait, les activités physico- sportives peuvent se convertir en une bonne stratégie pour relever de manière efficace le défi qui consiste à atteindre une éducation inclusive et pour tous. Cet article présente une étude réalisée dans les centres éducatifs reconnus officiellement comme des centres ayant des classes d’accueil. Concrètement, elle montre les résultats obtenus de l’application d’un questionnaire destiné aux éducateurs (instituteurs, professeurs et autres agents éducatifs dans le but d’insister sur et de démontrer la valeur inclusive des activités physico-sportives réalisées en dehors des horaires d’enseignement.One direct consequence of the cultural diversity phenomenon is the huge influx of students to educational centres. In view of this fact, physico-sports activities can be used as a good strategy to effectively address the challenge of

  13. 师范教育数学专业数学课外阅读的思考%Reflections on Mathematics Extracurricular Reading in Normal Education

    Institute of Scientific and Technical Information of China (English)

    唐越桥

    2013-01-01

    At the same time to develop the necessary ideas, to adapt to the future engaged in mathematics education teaching needs.In normal colleges and universities mathematics education professional Settings of the humanities and social science course serious shortage situation, through the designated bibliography, arrange extracurricular reading task, the introduction of evaluation mechanism, organization reading activities and the reading activity of mathematical culture quality education is an important supplement.as well as enhance mathematical prowess and humanistic awareness, improve the mathematical competence in research and education, and contribute towards cultivating the qualified math teacher.%在师范院校数学教育专业设置的人文社会科学课程严重不足的情况下,通过指定书目,安排课外阅读任务,引入考核评价机制,组织阅读交流活动等课外阅读活动是对数学文化素质教育的重要补充,可以丰富学生的数学文化素养和人文素养,提高学生的数学教育研究能力和教学能力,有助于培养优秀的数学教师。

  14. Signalling pathways involved in the activation of dendritic cells by layered double hydroxide nanoparticles.

    Science.gov (United States)

    Li, Ang; Qin, Lili; Zhu, Di; Zhu, Rongrong; Sun, Jing; Wang, Shilong

    2010-02-01

    Layered double hydroxide (LDH) nanoparticles are attractive as potential drug vectors for the targeting not only of tissues, but also of intracellular organelles, and particularly the acidic endolysosomes created after cell endocytosis. The purpose of this study was to investigate the ability of LDH nanoparticles designed as vectors to activate dendritic cells (DCs), as measured by various cellular functions. The study also explored the possible signaling pathway through which the LDH nanoparticles exerted their effects on the cellular functions of DCs. First, LDH nanoparticles with different ratios of Mg(OH)(2) to Al(OH)(3) (1:1, 2:1 and 3:1, called R1, R2 and R3 respectively) were optimized and had a hydrodynamic diameter of 57 nm with a zeta potential of +35 mV. Then, the efficient endocytosis of the optimized LDH nanoparticles by bone marrow-derived dendritic cells (MDDCs) was monitored by fluorescence-activated cell sorting. The effect of R1, R2 and R3 on the expression of the pro- and anti-inflammatory cytokines (TNF-alpha, IL-6, and IL-12) and the co-stimulatory molecules (CD40, CD80, CD86, and MHC class II) in MDDCs was examined. The exposure of R1 caused a dose-dependent increase in the expression of TNF-alpha, IL-12, CD86 and CD40, while R2 and R3 did not up-regulate these cytokines and co-stimulatory molecules. Migration assays showed that R1 could increase the migration capacity of DCs to CCL21 and up-regulate the expression of CCR7. Furthermore, we found that R1 significantly increased the NF-kappaB expression in the nucleus (in a dose-dependent manner) and promoted the degradation of total IkappaBalpha levels, indicating that the NF-kappaB signaling pathway might involve in an R1-induced DC activation. Our results suggested that LDH nanoparticles, in the future, may function as a useful vector for ex vivo engineering to promote vaccine delivery in immune cells.

  15. Parasympathetic activation is involved in reducing epileptiform discharges when listening to Mozart music.

    Science.gov (United States)

    Lin, Lung-Chang; Chiang, Ching-Tai; Lee, Mei-Wen; Mok, Hin-Kiu; Yang, Yi-Hsin; Wu, Hui-Chuan; Tsai, Chin-Lin; Yang, Rei-Cheng

    2013-08-01

    discharges in children with epilepsy. The majority of these patients showed an increase in parasympathetic tone during music exposure. Our results suggested that Mozart music stimuli induced parasympathetic activation which may be involved in the effect of music in reducing epileptiform discharges and the recurrence rate of seizures. Copyright © 2013 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.

  16. Programa de Actividad Física Extracurricular en Adolescentes con Sobrepeso u Obesidad

    Directory of Open Access Journals (Sweden)

    Miguel Tortosa-Martínez

    2016-01-01

    Full Text Available Nuestro objetivo en este estudio es comprobar la eficacia de un programa de actividad físico-deportiva (PAF extracurricular, en un grupo de adolescentes con sobrepesoobesidad, durante un período de 6 meses, respecto al consumo máximo de oxígeno, frecuencia cardíaca de recuperación, presión arterial e índice de grasa cintura/cadera, y ver las diferencias en cuanto al género. El estudio lo realizamos con 38 estudiantes de primer ciclo de secundaria, con un IMC superior al percentil 85. Los resultados reflejan mejoras significativas en el grupo experimental (GE, tras la realización del PAF, comparado con el grupo control (GC. Los componentes del GE mejoran en las variables fisiológicas y antropométricas, y también en función del género. Por otro lado, los datos obtenidos por el GC, reflejan una leve mejora en cuanto al VO2max, pero no en el resto de variables. Estos resultados sugieren la importancia de un adecuado programa de actividad físico-deportiva como contribución a mejorar parámetros cardiovasculares en adolescentes.

  17. Programa de Actividad Física Extracurricular en Adolescentes con Sobrepeso u Obesidad

    Directory of Open Access Journals (Sweden)

    Miguel Tortosa Martínez

    2016-02-01

    Full Text Available Nuestro objetivo en este estudio es comprobar la eficacia de un programa de actividad físico-deportiva (PAF extracurricular, en un grupo de adolescentes con sobrepeso obesidad, durante un período de 6 meses, respecto al consumo máximo de oxígeno, frecuencia cardíaca de recuperación, presión arterial e índice de grasa cintura/cadera, y ver las diferencias en cuanto al género. El estudio lo realizamos con 38 estudiantes de primer ciclo de secundaria, con un IMC superior al percentil 85. Los resultados reflejan mejoras significativas en el grupo experimental (GE, tras la realización del PAF, comparado con el grupo control (GC. Los componentes del GE mejoran en las variables fisiológicas y antropométricas, y también en función del género. Por otro lado, los datos obtenidos por el GC, reflejan una leve mejora en cuanto al VO2max, pero no en el resto de variables. Estos resultados sugieren la importancia de un adecuado programa de actividad físico-deportiva como contribución a mejorar parámetros cardiovasculares en adolescentes.

  18. Extracurricular factors influence perceived stress in a large cohort of Colombian dental students.

    Science.gov (United States)

    Divaris, Kimon; Polychronopoulou, Argy; Villa-Torres, Laura; Mafla, Ana Cristina; Moya, Gloria Aranzazu; González-Martínez, Farith; Vila-Sierra, Luis Armando; Fortich-Mesa, Natalia; Gómez-Scarpetta, Ruth Ángela; Duque-Restrepo, Liliana María

    2014-02-01

    The aim of this study was to investigate the association of extracurricular factors including socioeconomic status and career choice with perceived stress in dental school in a large cohort of Colombian dental students. Participants in the study were 5,700 students enrolled in seventeen Colombian dental schools. The study employed a Spanish adaptation of the Dental Environment Stressors (DES30-Sp) questionnaire and recorded an array of demographic, socioeconomic, career choice, and dental studies-related information. Data analyses relied on descriptive, bivariate, and multivariate methods based on multi-level mixed-effects linear regression and post hoc estimation of predictive margins. "Fear of failing a course or year" emerged as the highest ranked item. Male students consistently reported less perceived stress than females, and stress scores were higher among seniors. Independent of gender, age, and study year, having dentistry as one's first career choice, relying on financial support, and belonging to higher socioeconomic strata were associated with lower stress levels. Academic environment interventions aimed to improve students' educational well-being will need to account for the individual heterogeneity among them. These data from a robust cohort of predoctoral dental students underscore the importance of considering students' educational experiences in a broader social and economic context.

  19. Activated Rho kinase mediates diabetes-induced elevation of vascular arginase activation and contributes to impaired corpora cavernosa relaxation: possible involvement of p38 MAPK activation.

    Science.gov (United States)

    Toque, Haroldo A; Nunes, Kenia P; Yao, Lin; Liao, James K; Webb, R Clinton; Caldwell, Ruth B; Caldwell, R William

    2013-06-01

    Activated RhoA/Rho kinase (ROCK) has been implicated in diabetes-induced erectile dysfunction. Earlier studies have demonstrated involvement of ROCK pathway in the activation of arginase in endothelial cells. However, signaling pathways activated by ROCK in the penis remain unclear. We tested whether ROCK and p38 MAPK are involved in the elevation of arginase activity and subsequent impairment of corpora cavernosal (CC) relaxation in diabetes. Eight weeks after streptozotocin-induced diabetes, vascular functional studies, arginase activity assay, and protein expression of RhoA, ROCK, phospho-p38 MAPK, p38 MAPK, phospho-MYPT-1(Thr850), MYPT-1 and arginase levels were assessed in CC tissues from nondiabetic wild type (WT), diabetic (D) WT (WT + D), partial ROCK 2(+/-) knockout (KO), and ROCK 2(+/-) KO + D mice. The expression of RhoA, ROCK 1 and 2, phosphorylation of MYPT-1(Thr850) and p38 MAPK, arginase activity/expression, endothelial- and nitrergic-dependent relaxation of CC was assayed. Diabetes significantly reduced maximum relaxation (Emax ) to both endothelium-dependent acetylcholine (WT + D: Emax; 61 ± 4% vs. WT: Emax; 75 ± 2%) and nitrergic nerve stimulation. These effects were associated with increased expression of active RhoA, ROCK 2, phospho-MYPT-1(Thr850), phospho-p38 MAPK, arginase II, and activity of corporal arginase (1.6-fold) in WT diabetic CC. However, this impairment in CC of WT + D mice was absent in heterozygous ROCK 2(+/-) KO + D mice for acetylcholine (Emax : 80 ± 5%) and attenuated for nitrergic nerve-induced relaxation. CC of ROCK 2(+/-) KO + D mice showed much less ROCK activity, did not exhibit p38 MAPK activation, and had reduced arginase activity and arginase II expression. These findings indicate that ROCK 2 mediates diabetes-induced elevation of arginase activity. Additionally, pretreatment of WT diabetic CC with inhibitors of arginase (ABH) or p38 MAPK (SB203580) partially prevented impairment of ACh- and nitrergic nerve

  20. Residues involved in the pore-forming activity of the Clostridium perfringens iota toxin.

    Science.gov (United States)

    Knapp, Oliver; Maier, Elke; Waltenberger, Eva; Mazuet, Christelle; Benz, Roland; Popoff, Michel R

    2015-02-01

    Clostridium perfringens iota toxin is a binary toxin that is organized into enzyme (Ia) and binding (Ib) components. Ib forms channels in lipid bilayers and mediates the transport of Ia into the target cells. Here we show that Ib residues 334-359 contain a conserved pattern of alternating hydrophobic and hydrophilic residues forming two amphipathic β-strands involved in membrane insertion and channel formation. This stretch of amino acids shows remarkable structural and functional analogies with the β-pore-forming domain of C. perfringens epsilon toxin. Several mutations within the two amphipathic β-strands affected pore formation, single-channel conductance and ion selectivity (S339E-S341E, Q345H N346E) confirming their involvement in channel formation. F454 of Ib corresponds to the Φ-clamp F427 of anthrax protective antigen and F428 of C2II binary toxins. The mutation F454A resulted in a loss of cytotoxicity and strong increase in single-channel conductance (500 pS as compared with 85 pS in 1 M KCl) with a slight decrease in cation selectivity, indicating that the Φ-clamp is highly conserved and crucial for binary toxin activity. In contrast, the mutants Q367D, N430D, L443E had no or only minor effects on Ib properties, while T360I, T360A and T360W caused a dramatic effect on ion selectivity and single-channel conductance, indicating gross disturbance of the oligomer structure. This suggests that, at least in the iota toxin family, T360 has a structural role in the pore organization. Moreover, introduction of charged residues within the channel (S339E-S341E) or in the vestibule (Q367D, N430D and L443E) had virtually no effect on chloroquine or Ia binding, whereas F454A, T360I, T360A and T360W strongly decreased the chloroquine and Ia affinity to Ib. These results support that distinct residues within the vestibule interact with chloroquine and Ia or are responsible for channel structure, while the channel lining amino acids play a less important role.

  1. Rural Schooling in Georgia: The Experiences of a Minority Community Service Organization Involved in Local School Decision-Making Activities

    Science.gov (United States)

    Lowe, Cynthia Louise Altman

    2010-01-01

    This dissertation study was a descriptive case study of a minority community service organization whose members were actively involved in local school decision-making and activities in a rural Northeast Georgia community. Rural schools face unique challenges in light of current educational trends. To address the challenges, rural schools must…

  2. Rural Schooling in Georgia: The Experiences of a Minority Community Service Organization Involved in Local School Decision-Making Activities

    Science.gov (United States)

    Lowe, Cynthia Louise Altman

    2010-01-01

    This dissertation study was a descriptive case study of a minority community service organization whose members were actively involved in local school decision-making and activities in a rural Northeast Georgia community. Rural schools face unique challenges in light of current educational trends. To address the challenges, rural schools must…

  3. Antifungal Activity of Apple Cider Vinegar on Candida Species Involved in Denture Stomatitis.

    Science.gov (United States)

    Mota, Ana Carolina Loureiro Gama; de Castro, Ricardo Dias; de Araújo Oliveira, Julyana; de Oliveira Lima, Edeltrudes

    2015-06-01

    To evaluate the in vitro antifungal activity of apple cider vinegar on Candida spp. involved in denture stomatitis. The microdilution technique was used to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of apple cider vinegar containing 4% maleic acid, and nystatin (control). Further tests of microbial kinetics and inhibition of adherence to acrylic resin were performed testing different concentrations (MIC, MICx2, MICx4) of the products at time intervals of 0, 30, 60, 120 and 180 minutes. A roughness meter was used to measure the changes in surface roughness; color change of the acrylic resin specimens exposed to the test products in different concentrations and time intervals were also evaluated. Apple cider vinegar (4%) showed MIC of 2500 μg/ml and MFC of 2500, 5000, and 10,000 μg/ml depending on the strain tested. Nystatin showed MIC of 3.125 μg/ml and strain-dependent MFC values ranging from 3.125 to 12.5 μg/ml. The microbial kinetic assay showed a statistical difference between apple cider vinegar and nystatin (p cider vinegar showed fungicidal effect at MICx4, whereas nystatin maintained its fungistatic effect. Apple cider vinegar showed greater inhibition of adherence (p cider vinegar did not significantly alter the surface roughness of the acrylic resin specimens compared to nystatin (p > 0.05), and both had no influence on their color. Apple cider vinegar showed antifungal properties against Candida spp., thus representing a possible therapeutic alternative for patients with denture stomatitis. © 2014 by the American College of Prosthodontists.

  4. Active involvement of people with intellectual disabilities in health research - A structured literature review.

    Science.gov (United States)

    Frankena, Tessa Kim; Naaldenberg, Jenneken; Cardol, Mieke; Linehan, Christine; van Schrojenstein Lantman-de Valk, Henny

    2015-01-01

    Actively involving people with intellectual disabilities (ID) in health research, also known as inclusive health research, is increasingly popular. Currently, insight into experiences of this type of research is scarce. To gain insight into this topic, a structured literature review was conducted focussing on (1) existing theories, (2) inclusive methods, (3) added value and (4) barriers and facilitators. Literature published between January 2000 and January 2014 was included covering keywords related to ID and inclusive health research. Searches were performed in Pubmed, CINAHL, PsycINFO, EMBASE and MEDLINE databases, resulting in 26 included papers. Papers were quality assessed and analysed using qualitative data analysis software. Four theories were often simultaneously addressed: participatory research, emancipatory research, inclusive research and Arnstein's ladder. Barriers and facilitators could be divided into preparing, undertaking and finalising phases of research. Authors indicated that their motivation to conduct inclusive health research was based on demands by policy and funding bodies or was based on ethical considerations (i.e., ethical notions and giving people with ID a voice). Upon completion, authors perceived increased quality and validity of their research and several benefits for stakeholders (i.e., people with ID, researchers and healthcare professionals). Overall, there was consistency in their perception of the most important aspects of inclusive health research. Based on the analysis of included papers, four recommendations of inclusive health research with people with ID were found. Inclusive health research should be: (1) tailoring to the specific study; (2) anticipating all stakeholders; (3) considering its added value; and (4) providing insight into its process.

  5. Mothers' and fathers' involvement with school-age children's care and academic activities in Navajo Indian families.

    Science.gov (United States)

    Hossain, Ziarat; Anziano, Michael C

    2008-04-01

    This exploratory study examined mothers' and fathers' reports of time involvement in their school-age children's care and academic activities. The study also explored the relationship between parents' socioeconomic status (SES) variables (age, education, income, work hours, and length of marriage) and their relative involvement with children. Mother and father dyads from 34 two-parent Navajo (Diné) Indian families with a second- or third-grade child participated in the study. Repeated measures analysis of variance showed that mothers invested significantly more time in children's care on demand and academic activities than fathers, but the differences in maternal and paternal perceptions of time involvement in routine care were not significant. The gender of the child did not influence the amount of time parents invested in children's care and academic activities. Mothers' involvement with children was not related to any of the SES variables. Fathers' involvement was significantly associated with work hours and length of marriage, and work hours produced significant interaction with fathers' involvement with children. Findings are discussed in light of gender role differences in parental involvement with children within Navajo families.

  6. Correlation of Pulmonary Involvement with Serum Anti-CCP Antibodies and Disease Activity in Patients with Rheumatoid Arthritis

    Directory of Open Access Journals (Sweden)

    Songül Çilda¤

    2010-12-01

    Full Text Available Aim: Lung involvement substantially increases the morbidity and mortality rates in patients with rheumatoid arthritis (RA, thus, the early detection of lung involvement is essential for proper management. Several recent reports revealed that anti-CCP is an important parameter in the early diagnosis of RA and is closely related with the extraarticular manifestations of the disease. The aim of this study was to determine the relationship between serum anti-CCP antibodies and disease activity score-28 (DAS28, using the Stanford Health Assessment Questionnaire (HAQ in patients with RA, whose pulmonary involvement was detected by computed tomography (CT. Methods: According to the high-resolution CT findings, the patients were divided into two groups - with pulmonary involvement (24 patients, and without a pulmonary disease (25 patients. Results: Statistically significant association was not found between pulmonary involvement and anti-CCP antibodies. There was no significant correlation between pulmonary involvement and disease activity evaluated by the HAQ. Conclusion: The disease activity determined by the DAS28 was significantly negatively correlated with pulmonary involvement (The Medical Bulletin of Haseki 2010; 48: 142-5

  7. Dexamethasone enhances necrosis-like neuronal death in ischemic rat hippocampus involving μ-calpain activation.

    Science.gov (United States)

    Müller, Georg Johannes; Hasseldam, Henrik; Rasmussen, Rune Skovgaard; Johansen, Flemming Fryd

    2014-11-01

    Transient forebrain ischemia (TFI) leads to hippocampal CA1 pyramidal cell death which is aggravated by glucocorticoids (GC). It is unknown how GC affect apoptosis and necrosis in cerebral ischemia. We therefore investigated the co-localization of activated caspase-3 (casp-3) with apoptosis- and necrosis-like cell death morphologies in CA1 of rats treated with dexamethasone prior to TFI (DPTI). In addition, apoptosis- (casp-9, casp-3, casp-3-cleaved PARP and cleaved α-spectrin 145/150 and 120kDa) and necrosis-related (calpain-specific casp-9 cleavage, μ-calpain upregulation and cleaved α-spectrin 145/150kDa) cell death mechanisms were investigated by Western blot analysis. DPTI expedited CA1 neuronal death from day 4 to day 1 and increased the magnitude of CA1 neuronal death from 66.2% to 91.3% at day 7. Furthermore, DPTI decreased the overall (days 1-7) percentage of dying neurons displaying apoptosis-like morphology from 4.7% to 0.3% and, conversely, increased the percentage of neurons with necrosis-like morphology from 95.3% to 99.7%. In animals subjected to TFI without dexamethasone (ischemia-only), 7.4% of all dying CA1 neurons were casp-3-immunoreactive (IR), of which 3.1% co-localized with apoptosis-like and 4.3% with necrosis-like changes. By contrast, DPTI decreased the percentage of dying neurons with casp-3 IR to 1.4%, of which 0.3% co-localized with apoptosis-like changes and 1.1% with necrosis-like changes. Western blot analysis from DPTI animals showed a significant elevation of μ-calpain, a calpain-produced necrosis-related casp-9 fragment (25kDa) and cleavage of α-spectrin into 145/150kDa fragments at day 4, whereas in ischemia-only animals a significant increase of casp-3-cleaved PARP, cleavage of α-spectrin into 145/150 and 120kDa fragments was detected at day 7. We conclude that DPTI, in addition to augmenting and expediting CA1 neuronal death, causes a shift from apoptosis-like cell death to necrosis involving μ-calpain activation.

  8. Family involvement in music impacts participation of children with cochlear implants in music education and music activities

    Science.gov (United States)

    Driscoll, Virginia; Gfeller, Kate; Tan, Xueli; See, Rachel L.; Cheng, Hsin-Yi; Kanemitsu, Mikiko

    2014-01-01

    Objective Children with cochlear implants (CIs) participate in musical activities in school and daily lives. Considerable variability exists regarding the amount of music involvement and enjoyment. Using the Music Engagement Questionnaire-Preschool/Elementary (MEQ-P/E), we wanted to determine patterns of musical participation and the impact of familial factors on engagement. Methods Parents of 32 children with CIs (16 preschool, 16 elementary) completed a questionnaire regarding the musical involvement of their child with an implant and a normal-hearing (NH) sibling (if one existed). We compared CI children's involvement to that of their NH siblings as well as across groups of children with and without CIs. Correlations between parent ratings of music importance, demographic factors, and involvement of CI and NH children were conducted within and across groups. Results No significant differences were found between children with CIs and NH siblings, meaning children from the same family showed similar levels of musical involvement. When compared at the same developmental stage, no significant differences were found between preschool children with and without CIs. Parents who rated the importance of music as “low” or “middle” had children (NH and CI) who were less involved in music activities. Children whose parents rated music importance as “high” were involved in monthly to weekly music activities with 81.25% reporting daily music listening. Conclusion Despite a less-than-ideal auditory signal for music, preschool and school-aged CI children enjoy and are involved in musical experiences. Families who enjoy and spend a greater amount of time involved in music tend to have children who also engage more actively in music. PMID:25431978

  9. Family involvement in music impacts participation of children with cochlear implants in music education and music activities.

    Science.gov (United States)

    Driscoll, Virginia; Gfeller, Kate; Tan, Xueli; See, Rachel L; Cheng, Hsin-Yi; Kanemitsu, Mikiko

    2015-05-01

    Objective Children with cochlear implants (CIs) participate in musical activities in school and daily lives. Considerable variability exists regarding the amount of music involvement and enjoyment. Using the Music Engagement Questionnaire-Preschool/Elementary (MEQ-P/E), we wanted to determine patterns of musical participation and the impact of familial factors on engagement. Methods Parents of 32 children with CIs (16 preschool and 16 elementary) completed a questionnaire regarding the musical involvement of their child with an implant and a normal-hearing (NH) sibling (if one existed). We compared CI children's involvement to that of their NH siblings as well as across groups of children with and without CIs. Correlations between parent ratings of music importance, demographic factors, and involvement of CI and NH children were conducted within and across groups. Results No significant differences were found between children with CIs and NH siblings, meaning children from the same family showed similar levels of musical involvement. When compared at the same developmental stage, no significant differences were found between preschool children with and without CIs. Parents who rated the importance of music as 'low' or 'middle' had children (NH and CI) who were less involved in music activities. Children whose parents rated music importance as 'high' were involved in monthly to weekly music activities with 81.25% reporting daily music listening. Conclusion Despite a less-than-ideal auditory signal for music, preschool and school-aged CI children enjoy and are involved in musical experiences. Families who enjoy and spend a greater amount of time involved in music tend to have children who also engage more actively in music.

  10. Differential Involvement of Amygdala and Cortical NMDA Receptors Activation upon Encoding in Odor Fear Memory

    Science.gov (United States)

    Hegoburu, Chloé; Parrot, Sandrine; Ferreira, Guilaume; Mouly, Anne-Marie

    2014-01-01

    Although the basolateral amygdala (BLA) plays a crucial role for the acquisition of fear memories, sensory cortices are involved in their long-term storage in rats. However, the time course of their respective involvement has received little investigation. Here we assessed the role of the glutamatergic N-methyl-D-aspartate (NMDA) receptors in the…

  11. Differential Involvement of Amygdala and Cortical NMDA Receptors Activation upon Encoding in Odor Fear Memory

    Science.gov (United States)

    Hegoburu, Chloé; Parrot, Sandrine; Ferreira, Guilaume; Mouly, Anne-Marie

    2014-01-01

    Although the basolateral amygdala (BLA) plays a crucial role for the acquisition of fear memories, sensory cortices are involved in their long-term storage in rats. However, the time course of their respective involvement has received little investigation. Here we assessed the role of the glutamatergic N-methyl-D-aspartate (NMDA) receptors in the…

  12. Enhancing Information Usefulness by Line Managers’ Involvement in Cross-Unit Activities

    DEFF Research Database (Denmark)

    Foss, Kirsten; Rodgers, Waymond

    2011-01-01

    of line managers’ prior involvement with Corporate Audit using Throughput Modeling. This model allows us to understand how line managers’ cross-unit involvement influenced the way they process information received from Corporate Audit. Our results show that managers’ cross-unit involvement positively...... influences their assessment of information from Corporate Audit in a way that influences their propensity to use information from that unit. The results indicate that cross-unit involvement is more than an effective means of transmitting information – it can also be used as a means of building boundary......Organization and management scholars have long advocated that efficient use of information is critical for firms to compete successfully in the modern marketplace. This study examines whether the use of managerial cross-unit involvement in an organization enhances managers’ propensity to use useful...

  13. The theory of active involvement: processes underlying interventions that engage adolescents in message planning and/or production.

    Science.gov (United States)

    Greene, Kathryn

    2013-01-01

    Adolescence is a time of increased risk taking, and recent intervention strategies have included adolescents planning or producing antirisk messages for their peers. Although these projects may generate enthusiasm, we know little about message planning or production as a strategy for changing adolescent decision-making and behavior. This article articulates the Theory of Active Involvement (TAI) to describe and explain the processes through which these active involvement interventions influence adolescents. TAI is based on social cognitive theory's notion of self-regulation and examines multiple perspective taking and activating the self-reflection processes. The theory specifically describes the process of cognitive changes experienced by participants in active involvement interventions. The sequence is conceptualized as starting when engagement with the intervention (arousal and involvement) produces skill and knowledge gains (immediate outcomes) that lead to reflection (perceived discrepancy) and then other cognitions (expectancies, norms, intentions), with the ultimate outcome being behavior change. Engaging the target audience in a process of self-reflection is conceptualized as the crucial ingredient for meaningful and sustainable change in cognitions and behavior. This article provides valuable insight into how active involvement strategies function and how to best design these interventions, particularly those targeting adolescents.

  14. The link between high-fat meals and postprandial activation of blood coagulation factor VII possibly involves kallikrein

    DEFF Research Database (Denmark)

    Larsen, L F; Marckmann, P; Bladbjerg, Else-Marie

    2000-01-01

    Contrary to low-fat meals, high-fat meals are known to cause postprandial factor VII (FVII) activation, but the mechanism is unknown. To study the postprandial FVII activation in detail, 18 young men consumed in randomized order high-fat or low-fat test meals. Fasting and non-fasting blood samples...... that triglyceride-rich lipoproteins may activate prokallikrein. Neither plasma triglycerides nor kallikrein and activated FVII were statistically associated. This may suggest that additional factors are involved in the postprandial FVII activation. No clear evidence for a role of tissue factor expression...

  15. Extracurricular activity participation moderates impact of family and school factors on adolescents’ disruptive behavioural problems

    OpenAIRE

    Driessens, Corine M.E.F.

    2015-01-01

    Background The prevalence of problem behaviours among British adolescents has increased in the past decades. Following Erikson’s psychosocial developmental theory and Bronfenbrenner’s developmental ecological model, it was hypothesized that youth problem behaviour is shaped in part by social environment. The aim of this project was to explore potential protective factors within the social environment of British youth’s for the presentation of disruptive behavioural problems. Method This study...

  16. Extracurricular Activities and the Development of Social Skills in Children with Intellectual and Specific Learning Disabilities

    Science.gov (United States)

    Brooks, B. A.; Floyd, F.; Robins, D. L.; Chan, W. Y.

    2015-01-01

    Background: Children with intellectual disability and specific learning disabilities often lack age-appropriate social skills, which disrupts their social functioning. Because of the limited effectiveness of classroom mainstreaming and social skills training for these children, it is important to explore alternative opportunities for social skill…

  17. Are Students More Engaged When Schools Offer Extracurricular Activities? PISA in Focus. No. 18

    Science.gov (United States)

    OECD Publishing (NJ1), 2012

    2012-01-01

    Are students more engaged and do they perform better in science if their school encourages them to work on science projects, participate in science fairs, belong to a science-related club or go on science-related field trips--in addition to teaching them the mandatory science curriculum? To find out, PISA (Programme for International Student…

  18. Extracurricular activity participation moderates impact of family and school factors on adolescents’ disruptive behavioural problems

    OpenAIRE

    Driessens, Corine M.E.F.

    2015-01-01

    Background The prevalence of problem behaviours among British adolescents has increased in the past decades. Following Erikson’s psychosocial developmental theory and Bronfenbrenner’s developmental ecological model, it was hypothesized that youth problem behaviour is shaped in part by social environment. The aim of this project was to explore potential protective factors within the social environment of British youth’s for the presentation of disruptive behavioural problems. Method This study...

  19. Personal involvement is related to increased search motivation and associated with activity in left BA44 - a pilot study

    OpenAIRE

    Michael eSchaefer; Franziska eRumpel; Abdolkarim eSadrieh; Martin eReimann; Claudia eDenke

    2015-01-01

    Numerous studies explore consumer perception of brands in a more or less passive way. This may still be representative for many situations or decisions we make each day. Nevertheless, sometimes we often actively search for and use information to make informed and reasoned choices, thus implying a rational and thinking consumer. Researchers suggested describing this distinction as low relative to high involvement consumer behavior. Although the involvement concept has been widely used to expla...

  20. Personal involvement is related to increased search motivation and associated with activity in left BA44 - a pilot study

    Directory of Open Access Journals (Sweden)

    Michael eSchaefer

    2015-03-01

    Full Text Available Numerous studies explore consumer perception of brands in a more or less passive way. This may still be representative for many situations or decisions we make each day. Nevertheless, sometimes we often actively search for and use information to make informed and reasoned choices, thus implying a rational and thinking consumer. Researchers suggested describing this distinction as low relative to high involvement consumer behavior. Although the involvement concept has been widely used to explain consumer behavior, behavioral and neural correlates of this concept are poorly understood. The current study aims to describe a behavioral measure that is associated with high involvement, the length of search behavior. A second aim of this study was to explore brain activations associated with involvement by employing functional magnetic resonance imaging (fMRI. We presented participants information cues for different products and told them that they had to answer questions with respect to these products at the end of the experiment. Participants were free to stop the information search if they think they gathered enough information or to continue with collecting information. Behavioral results confirmed our hypothesis of a relationship between searching behavior and personal involvement by demonstrating that the length of search correlated significantly with the degree of personal involvement of the participants. FMRI data revealed that personal involvement was associated with activation in BA44. Since this brain region is known to be involved in semantic memory, the results of this pilot study suggest that high involvement consumer behavior may be linked to cognitive load and attention towards a product.

  1. Personal involvement is related to increased search motivation and associated with activity in left BA44-a pilot study.

    Science.gov (United States)

    Schaefer, Michael; Rumpel, Franziska; Sadrieh, Abdolkarim; Reimann, Martin; Denke, Claudia

    2015-01-01

    Numerous studies explore consumer perception of brands in a more or less passive way. This may still be representative for many situations or decisions we make each day. Nevertheless, sometimes we often actively search for and use information to make informed and reasoned choices, thus implying a rational and thinking consumer. Researchers suggested describing this distinction as low relative to high involvement consumer behavior. Although the involvement concept has been widely used to explain consumer behavior, behavioral and neural correlates of this concept are poorly understood. The current study aims to describe a behavioral measure that is associated with high involvement, the length of search behavior. A second aim of this study was to explore brain activations associated with involvement by employing functional magnetic resonance imaging (fMRI). We presented participants information cues for different products and told them that they had to answer questions with respect to these products at the end of the experiment. Participants were free to stop the information search if they think they gathered enough information or to continue with collecting information. Behavioral results confirmed our hypothesis of a relationship between searching behavior and personal involvement by demonstrating that the length of search correlated significantly with the degree of personal involvement of the participants. fMRI data revealed that personal involvement was associated with activation in BA44. Since this brain region is known to be involved in semantic memory, the results of this pilot study suggest that high involvement consumer behavior may be linked to cognitive load and attention towards a product.

  2. Male Involvement in Child Care Activities: A Review of the Literature ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    cultural stereotypes and harmonization of customary and common laws. (Afr J Reprod ... and men's gender roles and advocated for men to take more .... In Botswana, the current data ..... data collection and analysis of male involvement in.

  3. We decide what we eat: Active involvement of students in developing school health policies

    DEFF Research Database (Denmark)

    Jensen, Bjarne Bruun

    2003-01-01

    The article explores different forms of participation related to youngs people's involvement in among other nutrition projects. Furthermore, the notion of action-oriented knowledge is discussed on the basis of a model.......The article explores different forms of participation related to youngs people's involvement in among other nutrition projects. Furthermore, the notion of action-oriented knowledge is discussed on the basis of a model....

  4. Psychological Health Education in Extracurricular Reading%如何在初中课外阅读中实现心理健康教育

    Institute of Scientific and Technical Information of China (English)

    毛瑞兰

    2016-01-01

    For junior middle school after-class reading is thoughtful, humanistic and comprehensive, so it is easy to promote students' mental health. There is a connection between text experience and mind cultivation in extracurricular reading. How to promote students' mental health in extracurricular reading is an important proposition. Now the author mainly discusses how to realize mental health education in students' extracurricular reading.%课外阅读在文本体验和培育心灵之间存在着一种必然的联系。在课外阅读中如何促进学生心理健康是一个值得探究的命题。现在我就借自己指导学生课外阅读的过程来谈谈实现心理健康教育的途径。

  5. Neuron-Glia Crosstalk and Neuropathic Pain: Involvement in the Modulation of Motor Activity in the Orofacial Region.

    Science.gov (United States)

    Hossain, Mohammad Zakir; Unno, Shumpei; Ando, Hiroshi; Masuda, Yuji; Kitagawa, Junichi

    2017-09-26

    Neuropathic orofacial pain (NOP) is a debilitating condition. Although the pathophysiology remains unclear, accumulating evidence suggests the involvement of multiple mechanisms in the development of neuropathic pain. Recently, glial cells have been shown to play a key pathogenetic role. Nerve injury leads to an immune response near the site of injury. Satellite glial cells are activated in the peripheral ganglia. Various neural and immune mediators, released at the central terminals of primary afferents, lead to the sensitization of postsynaptic neurons and the activation of glia. The activated glia, in turn, release pro-inflammatory factors, further sensitizing the neurons, and resulting in central sensitization. Recently, we observed the involvement of glia in the alteration of orofacial motor activity in NOP. Microglia and astroglia were activated in the trigeminal sensory and motor nuclei, in parallel with altered motor functions and a decreased pain threshold. A microglial blocker attenuated the reduction in pain threshold, reduced the number of activated microglia, and restored motor activity. We also found an involvement of the astroglial glutamate-glutamine shuttle in the trigeminal motor nucleus in the alteration of the jaw reflex. Neuron-glia crosstalk thus plays an important role in the development of pain and altered motor activity in NOP.

  6. Propuesta Educativa de Enriquecimiento Extracurricular y su Implementación: PEEEI

    Directory of Open Access Journals (Sweden)

    Krissia Morales Chacón

    2014-09-01

    Full Text Available http://dx.doi.org/10.5902/1984686X14322Este trabajo contiene un Plan de intervención psicoeducacional para niños superdotados con un abordaje multidimensional de acuerdo con el que la noción de inteligencia está integrada por múltiples componentes que son relativamente independientes pero que son esenciales para un desarrollo ideal de niños superdotados. Su objetivo general es implementar una propuesta educativa de enriquecimiento extracurricular – EPEEI dirigido al cuidado integral de niños superdotados en edades tempranas, a través de su aplicación en el campo. El método utilizado se desarrolló en dos etapas. La primera etapa estaba compuesta de tres campos de intervención: a Programas de psicología individual para incentivar a los niños de acuerdo a sus fortalezas; b Actividades de apoyo y capacitación para padres sobre el tema de niños superdotados; c Orientación y capacitación docente. La segunda etapa consistió en probar el plan durante un período de aproximadamente 6 meses. De acuerdo a los resultados, se comprobó que los niños superdotados en nuestro contexto, carecen de oportunidades psicopedagógicas que atiendan genuinamente a sus necesidades educacionales especiales. Tanto las escuelas como las familias carecen de la información y capacitación necesarias para los casos identificados. Ellos demuestran desorientación, frustración e ignorancia de los posibles servicios y recursos que podrían ofrecerles apoyo en esa tarea (Por ejemplo: Tecnologias de Información y comunicación.

  7. A disease activity score for ENT involvement in granulomatosis with polyangiitis (Wegener's)

    DEFF Research Database (Denmark)

    Del Pero, Marcos Martinez; Chaudhry, Afzal; Rasmussen, Niels

    2013-01-01

    Accurate assessment of disease activity in patients with otorhinolaryngological manifestations of granulomatosis with polyangiitis (Wegener's) (ENT/GPA) is necessary for treatment decisions and clinical trials. We have designed a disease activity score (ENT/GPA DAS) for this purpose....

  8. Individual differences in dispositional mindfulness and brain activity involved in reappraisal of emotion

    NARCIS (Netherlands)

    Modinos, Gemma; Ormel, Johan; Aleman, Andre

    2010-01-01

    The regulation of negative emotion through reappraisal has been shown to induce increased prefrontal activity and decreased amygdala activity. Individual differences in dispositional mindfulness reflect differences in typical recognition, detachment and regulation of current experience, thought to a

  9. Conditions of Dance in Morocco : Interviews and observations of Adults and Children involved in Dance activities in Marrakech

    OpenAIRE

    Karlsson, Linda

    2009-01-01

    The overall purpose of this study was to deepen our knowledge about dance in general and about dance activities for children in particular, in Morocco. Consequently, we attempted to gain insight and understanding of the social and political premises to dance in this context. We further intended to study how children were involved in dance activities. A qualitative study was carried out during a stay of eight weeks in Marrakech. Data was mainly collected through participant observations in the...

  10. Strategies for Effective Faculty Involvement in Online Activities Aimed at Promoting Critical Thinking and Deep Learning

    Science.gov (United States)

    Abdul Razzak, Nina

    2016-01-01

    Highly-traditional education systems that mainly offer what is known as "direct instruction" usually result in graduates with a surface approach to learning rather than a deep one. What is meant by deep-learning is learning that involves critical analysis, the linking of ideas and concepts, creative problem solving, and application…

  11. Aluminum hydroxide adjuvant differentially activates the three complement pathways with major involvement of the alternative pathway

    DEFF Research Database (Denmark)

    Güven, Esin; Duus, Karen; Laursen, Inga

    2013-01-01

    Al(OH)3 is the most common adjuvant in human vaccines, but its mode of action remains poorly understood. Complement involvement in the adjuvant properties of Al(OH)3 has been suggested in several reports together with a depot effect. It is here confirmed that Al(OH)3 treatment of serum depletes c...

  12. Strategies for Effective Faculty Involvement in Online Activities Aimed at Promoting Critical Thinking and Deep Learning

    Science.gov (United States)

    Abdul Razzak, Nina

    2016-01-01

    Highly-traditional education systems that mainly offer what is known as "direct instruction" usually result in graduates with a surface approach to learning rather than a deep one. What is meant by deep-learning is learning that involves critical analysis, the linking of ideas and concepts, creative problem solving, and application…

  13. Dilemmas of involvement in land management – Comparing an active (Dutch) and a passive (German) approach

    NARCIS (Netherlands)

    Thomas, Hartmann; Spit, Tejo

    2015-01-01

    What is the role of spatial planners in urban development? Planners can be very involved in the realization of the land-use plans or they can take a more passive role in development processes. Which role they take depends on the particular institutional arrangements for land management in each count

  14. Neural networks involved in adolescent reward processing: An activation likelihood estimation meta-analysis of functional neuroimaging studies.

    Science.gov (United States)

    Silverman, Merav H; Jedd, Kelly; Luciana, Monica

    2015-11-15

    Behavioral responses to, and the neural processing of, rewards change dramatically during adolescence and may contribute to observed increases in risk-taking during this developmental period. Functional MRI (fMRI) studies suggest differences between adolescents and adults in neural activation during reward processing, but findings are contradictory, and effects have been found in non-predicted directions. The current study uses an activation likelihood estimation (ALE) approach for quantitative meta-analysis of functional neuroimaging studies to: (1) confirm the network of brain regions involved in adolescents' reward processing, (2) identify regions involved in specific stages (anticipation, outcome) and valence (positive, negative) of reward processing, and (3) identify differences in activation likelihood between adolescent and adult reward-related brain activation. Results reveal a subcortical network of brain regions involved in adolescent reward processing similar to that found in adults with major hubs including the ventral and dorsal striatum, insula, and posterior cingulate cortex (PCC). Contrast analyses find that adolescents exhibit greater likelihood of activation in the insula while processing anticipation relative to outcome and greater likelihood of activation in the putamen and amygdala during outcome relative to anticipation. While processing positive compared to negative valence, adolescents show increased likelihood for activation in the posterior cingulate cortex (PCC) and ventral striatum. Contrasting adolescent reward processing with the existing ALE of adult reward processing reveals increased likelihood for activation in limbic, frontolimbic, and striatal regions in adolescents compared with adults. Unlike adolescents, adults also activate executive control regions of the frontal and parietal lobes. These findings support hypothesized elevations in motivated activity during adolescence.

  15. Expectations of Rock Music Consumption for Entertainment and Information Relative to the Active Involvement of the User.

    Science.gov (United States)

    Rouner, Donna; Noyes, Amy

    Before examining potentially negative effects of rock music on adolescents, it is necessary to demonstrate links between adolescent motivations for consuming rock music and active involvement relative to that use and also to consider how much rock listeners rely on rock music as a source for information about values, beliefs, and social…

  16. 30 CFR 57.4660 - Work in shafts, raises, or winzes and other activities involving hazard areas.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Work in shafts, raises, or winzes and other activities involving hazard areas. 57.4660 Section 57.4660 Mineral Resources MINE SAFETY AND HEALTH... non-fire-retardant wood. Thawing pipes electrically, except with heat tape More than 10 pounds...

  17. Parental Involvement in School Activities and Reading Literacy: Findings and Implications from PIRLS 2011 Data. Policy Brief No. 3

    Science.gov (United States)

    Mirazchiyski, Plamen; Klemencic, Eva

    2014-01-01

    This policy brief presents evidence demonstrating a positive association between parental involvement in school activities and student performance in the Progress in International Reading Literacy Study (PIRLS) 2011. This association, which was evident in most of the 54 education systems analyzed, indicates that students enrolled in schools with…

  18. High inorganic triphosphatase activities in bacteria and mammalian cells: identification of the enzymes involved.

    Directory of Open Access Journals (Sweden)

    Gregory Kohn

    Full Text Available BACKGROUND: We recently characterized a specific inorganic triphosphatase (PPPase from Nitrosomonas europaea. This enzyme belongs to the CYTH superfamily of proteins. Many bacterial members of this family are annotated as predicted adenylate cyclases, because one of the founding members is CyaB adenylate cyclase from A. hydrophila. The aim of the present study is to determine whether other members of the CYTH protein family also have a PPPase activity, if there are PPPase activities in animal tissues and what enzymes are responsible for these activities. METHODOLOGY/PRINCIPAL FINDINGS: Recombinant enzymes were expressed and purified as GST- or His-tagged fusion proteins and the enzyme activities were determined by measuring the release of inorganic phosphate. We show that the hitherto uncharacterized E. coli CYTH protein ygiF is a specific PPPase, but it contributes only marginally to the total PPPase activity in this organism, where the main enzyme responsible for hydrolysis of inorganic triphosphate (PPP(i is inorganic pyrophosphatase. We further show that CyaB hydrolyzes PPP(i but this activity is low compared to its adenylate cyclase activity. Finally we demonstrate a high PPPase activity in mammalian and quail tissue, particularly in the brain. We show that this activity is mainly due to Prune, an exopolyphosphatase overexpressed in metastatic tumors where it promotes cell motility. CONCLUSIONS AND GENERAL SIGNIFICANCE: We show for the first time that PPPase activities are widespread in bacteria and animals. We identified the enzymes responsible for these activities but we were unable to detect significant amounts of PPP(i in E. coli or brain extracts using ion chromatography and capillary electrophoresis. The role of these enzymes may be to hydrolyze PPP(i, which could be cytotoxic because of its high affinity for Ca(2+, thereby interfering with Ca(2+ signaling.

  19. Growth hormone-induced insulin resistance in human subjects involves reduced pyruvate dehydrogenase activity

    DEFF Research Database (Denmark)

    Nellemann, Birgitte; Vendelbo, Mikkel H; Nielsen, Thomas S

    2014-01-01

    Insulin resistance induced by growth hormone (GH) is linked to promotion of lipolysis by unknown mechanisms. We hypothesized that suppression of the activity of pyruvate dehydrogenase in the active form (PDHa) underlies GH-induced insulin resistance similar to what is observed during fasting....

  20. The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity

    DEFF Research Database (Denmark)

    Gordillo, M.; Vega, H.; Trainer, A.H.;

    2008-01-01

    on enzymatic activity and cellular phenotype. We found that ESCO2 W539G results in loss of autoacetyltransferase activity. The cellular phenotype produced by this mutation causes cohesion defects, proliferation capacity reduction and mitomycin C sensitivity equivalent to those produced by frameshift...

  1. Optical Topography of Evoked Brain Activity during Mental Tasks Involving Whole Number Operations

    Science.gov (United States)

    Ortiz, Enrique

    2014-01-01

    Students start to memorize arithmetic facts from early elementary school mathematics activities. Their fluency or lack of fluency with these facts could affect their efforts as they carry out mental calculations as adults. This study investigated participants' levels of brain activation and possible reasons for these levels as they solved…

  2. Teacher-Involved Conversations with Young Children during Small Group Activity

    Science.gov (United States)

    Durden, Tonia; Dangel, Julie Rainer

    2008-01-01

    This qualitative study examines the conversations of two preschool teachers with two- and three-year-old children during small-group activity settings in two high-quality child development centers. Using interviews, observations and videotaping of small-group activities, the conversations are characterized in terms of the kind and function of…

  3. A qualitative study of the activities performed by people involved in clinical decision support: recommended practices for success.

    Science.gov (United States)

    Wright, Adam; Ash, Joan S; Erickson, Jessica L; Wasserman, Joe; Bunce, Arwen; Stanescu, Ana; St Hilaire, Daniel; Panzenhagen, Morgan; Gebhardt, Eric; McMullen, Carmit; Middleton, Blackford; Sittig, Dean F

    2014-01-01

    To describe the activities performed by people involved in clinical decision support (CDS) at leading sites. We conducted ethnographic observations at seven diverse sites with a history of excellence in CDS using the Rapid Assessment Process and analyzed the data using a series of card sorts, informed by Linstone's Multiple Perspectives Model. We identified 18 activities and grouped them into four areas. Area 1: Fostering relationships across the organization, with activities (a) training and support, (b) visibility/presence on the floor, (c) liaising between people, (d) administration and leadership, (e) project management, (f) cheerleading/buy-in/sponsorship, (g) preparing for CDS implementation. Area 2: Assembling the system with activities (a) providing technical support, (b) CDS content development, (c) purchasing products from vendors (d) knowledge management, (e) system integration. Area 3: Using CDS to achieve the organization's goals with activities (a) reporting, (b) requirements-gathering/specifications, (c) monitoring CDS, (d) linking CDS to goals, (e) managing data. Area 4: Participation in external policy and standards activities (this area consists of only a single activity). We also identified a set of recommendations associated with these 18 activities. All 18 activities we identified were performed at all sites, although the way they were organized into roles differed substantially. We consider these activities critical to the success of a CDS program. A series of activities are performed by sites strong in CDS, and sites adopting CDS should ensure they incorporate these activities into their efforts.

  4. H2O2-Activated Up-Regulation of Glutathione in Arabidopsis Involves Induction of Genes Encoding Enzymes Involved in Cysteine Synthesis in the Chloroplast

    Institute of Scientific and Technical Information of China (English)

    Guillaume Queval; Dorothée Thominet; Hélène Vanacker; Myroslawa Miginiac-Maslow; Bertrand Gakière; Graham Noctor

    2009-01-01

    Glutathione is a key player in cellular redox homeostasis and, therefore, in the response to H2O2, but the factors regulating oxidation-activated glutathione synthesis are still unclear. We investigated H2O2-induced glutathione synthesis in a conditional Arabidopsis catalase-deficient mutant (cat2). Plants were grown from seed at elevated CO2 for 5 weeks, then transferred to air in either short-day or long-day conditions. Compared to cat2 at elevated CO2 or wild-type plants in any condition, transfer of cat2 to air in both photoperiods caused measurable oxidation of the leaf glutathione pool within hours. Oxidation continued on subsequent days and was accompanied by accumulation of glutathione. This effect was stronger in cat2 transferred to air in short days, and was not linked to appreciable increases in the extractable activities of or transcripts encoding enzymes involved in the committed pathway of glutathione synthesis. In contrast, it was accompanied by increases in serine, O-acetylserine, and cysteine. These changes in metabolites were accompanied by induction of genes encoding adenosine phosphosulfate reductase (APR), particularly APR3, as well as a specific serine acetyltransferase gene (SAT2.1) encoding a chloroplastic SAT. Marked induction of these genes was only observed in cat2 transferred to air in short-day conditions, where cysteine and glutathione accumulation was most dramatic. Unlike other SAT genes, which showed negligible induction in cat2, the relative abundance of APR and SAT2.1 transcripts was closely correlated with marker transcripts for H2O2 signaling. Together, the data underline the importance of cysteine synthesis in oxidant-induced up-regulation of glutathione synthesis and suggest that the chloroplast makes an important contribution to cysteine production under these circumstances.

  5. Involvement of lipid rafts in adhesion-induced activation of Met and EGFR

    Directory of Open Access Journals (Sweden)

    Lu Ying-Che

    2011-10-01

    Full Text Available Abstract Background Cell adhesion has been shown to induce activation of certain growth factor receptors in a ligand-independent manner. However, the mechanism for such activation remains obscure. Methods Human epidermal carcinoma A431 cells were used as a model to examine the mechanism for adhesion-induced activation of hepatocyte growth factor receptor Met and epidermal growth factor receptor (EGFR. The cells were suspended and replated on culture dishes under various conditions. The phosphorylation of Met at Y1234/1235 and EGFR at Y1173 were used as indicators for their activation. The distribution of the receptors and lipid rafts on the plasma membrane were visualized by confocal fluorescent microscopy and total internal reflection microscopy. Results We demonstrate that Met and EGFR are constitutively activated in A431 cells, which confers proliferative and invasive potentials to the cells. The ligand-independent activation of Met and EGFR in A431 cells relies on cell adhesion to a substratum, but is independent of cell spreading, extracellular matrix proteins, and substratum stiffness. This adhesion-induced activation of Met and EGFR cannot be attributed to Src activation, production of reactive oxygen species, and the integrity of the cytoskeleton. In addition, we demonstrate that Met and EGFR are independently activated upon cell adhesion. However, partial depletion of Met and EGFR prevents their activation upon cell adhesion, suggesting that overexpression of the receptors is a prerequisite for their self-activation upon cell adhesion. Although Met and EGFR are largely distributed in 0.04% Triton-insoluble fractions (i.e. raft fraction, their activated forms are detected mainly in 0.04% Triton-soluble fractions (i.e. non-raft fraction. Upon cell adhesion, lipid rafts are accumulated at the cell surface close to the cell-substratum interface, while Met and EGFR are mostly excluded from the membrane enriched by lipid rafts. Conclusions

  6. Activation of Hepatic Lipase Expression by Oleic Acid: Possible Involvement of USF1

    Directory of Open Access Journals (Sweden)

    Adrie J. M. Verhoeven

    2009-10-01

    Full Text Available Polyunsaturated fatty acids affect gene expression mainly through peroxisome proliferator-activated receptors (PPARs and sterol regulatory element binding proteins (SREBPs, but how monounsaturated fatty acids affect gene expression is poorly understood. In HepG2 cells, oleate supplementation has been shown to increase secretion of hepatic lipase (HL. We hypothesized that oleate affects HL gene expression at the transcriptional level. To test this, we studied the effect of oleate on HL promoter activity using HepG2 cells and the proximal HL promoter region (700 bp. Oleate increased HL expression and promoter activity 1.3–2.1 fold and reduced SREBP activity by 50%. Downregulation of SREBP activity by incubation with cholesterol+25-hydroxycholesterol had no effect on HL promoter activity. Overexpression of SREBP2, but not SREBP1, reduced HL promoter activity, which was effected mainly through the USF1 binding site at -307/-312. Oleate increased the nuclear abundance of USF1 protein 2.7 ± 0.6 fold, while USF1 levels were reduced by SREBP2 overexpression. We conclude that oleate increases HL gene expression via USF1. USF1 may be an additional fatty acid sensor in liver cells.

  7. Measuring the Activity of Leucine-Rich Repeat Kinase 2: A Kinase Involved in Parkinson's Disease

    Science.gov (United States)

    Lee, Byoung Dae; Li, Xiaojie; Dawson, Ted M.; Dawson, Valina L.

    2015-01-01

    Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson's disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated in the pathogenesis of Parkinson's disease. Developing an assay to understand the mechanisms of LRRK2 kinase activity is important for the development of pharmacologic and therapeutic applications. Here, we describe how to measure in vitro LRRK2 kinase activity and its inhibition. PMID:21960214

  8. Directory of DOE and contractor personnel involved in non-government standards activities

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    This document contains a listing of DOE employees and DOE contractors who have submitted form DOE F 1300.2, Record of Non-Government Standards Activity. Additional names were added from rosters supplied by non-Government standards bodies.

  9. Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway

    Energy Technology Data Exchange (ETDEWEB)

    Nagane, Masaki; Yasui, Hironobu; Sakai, Yuri; Yamamori, Tohru [Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818 (Japan); Niwa, Koichi [Laboratory of Biochemistry, Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493 (Japan); Hattori, Yuichi [Department of Molecular and Medical Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194 (Japan); Kondo, Takashi [Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194 (Japan); Inanami, Osamu, E-mail: inanami@vetmed.hokudai.ac.jp [Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818 (Japan)

    2015-01-02

    Highlights: • eNOS activity is increased in BAECs exposed to X-rays. • ATM is involved in this increased eNOS activity. • HSP90 modulates the radiation-induced activation of ATM and eNOS. - Abstract: In this study, the involvement of ataxia telangiectasia mutated (ATM) kinase and heat shock protein 90 (HSP90) in endothelial nitric oxide synthase (eNOS) activation was investigated in X-irradiated bovine aortic endothelial cells. The activity of nitric oxide synthase (NOS) and the phosphorylation of serine 1179 of eNOS (eNOS-Ser1179) were significantly increased in irradiated cells. The radiation-induced increases in NOS activity and eNOS-Ser1179 phosphorylation levels were significantly reduced by treatment with either an ATM inhibitor (Ku-60019) or an HSP90 inhibitor (geldanamycin). Geldanamycin was furthermore found to suppress the radiation-induced phosphorylation of ATM-Ser1181. Our results indicate that the radiation-induced eNOS activation in bovine aortic endothelial cells is regulated by ATM and HSP90.

  10. Aesthetic activities and aesthetic attitudes: influences of education, background and personality on interest and involvement in the arts.

    Science.gov (United States)

    McManus, I C; Furnham, A

    2006-11-01

    There have been few studies of why some people are frequently involved in aesthetic activities such as going to the theatre, reading or playing musical instruments, whereas others are less involved. This study assesses the broad roles of education, personality and demographic factors such as social class, age and sex. More aesthetic activity was associated with music and art education, whereas science education had a substantial negative relationship with aesthetic activity, both directly and also indirectly via reduced art education. More aesthetic activity was particularly related to higher scores on the personality factor of openness, and also to lower scores on agreeableness and conscientiousness. Higher parental social class was also associated with more aesthetic activity, as also was lower age. Sex had no relationship to aesthetic activity, as neither did masculinity-femininity. Positive aesthetic attitudes were also related moderately to aesthetic activity, but were particularly strongly related to openness to experience, and somewhat less to extraversion. Class, age and sex had no direct relationship to aesthetic attitudes.

  11. Involvement of endoplasmic reticulum stress in albuminuria induced inflammasome activation in renal proximal tubular cells.

    Directory of Open Access Journals (Sweden)

    Li Fang

    Full Text Available Albuminuria contributes to the progression of tubulointerstitial fibrosis. Although it has been demonstrated that ongoing albuminuria leads to tubular injury manifested by the overexpression of numerous proinflammatory cytokines, the mechanism remains largely unknown. In this study, we found that the inflammasome activation which has been recognized as one of the cornerstones of intracellular surveillance system was associated with the severity of albuminuria in the renal biopsies specimens. In vitro, bovine serum albumin (BSA could also induce the activation of NLRP3 inflammasome in the cultured kidney epithelial cells (NRK-52E. Since there was a significant overlap of NLRP3 with the ER marker calreticulin, the ER stress provoked by BSA seemed to play a crucial role in the activation of inflammasome. Here, we demonstrated that the chemical chaperone taurine-conjugated ursodeoxycholic acid (TUDCA which was proved to be an enhancer for the adaptive capacity of ER could attenuate the inflammasome activation induced by albuminuria not only in vitro but also in diabetic nephropathy. Taken together, these data suggested that ER stress seemed to play an important role in albuminuria-induced inflammasome activation, elimination of ER stress via TUDCA might hold promise as a novel avenue for preventing inflammasome activation ameliorating kidney epithelial cells injury induced by albuminuria.

  12. Leadership, management and teamwork learning through an extra-curricular project for medical students: descriptive study.

    Science.gov (United States)

    Jorge, Maria Lucia da Silva Germano; Coelho, Izabel Cristina Meister; Paraizo, Mariana Martins; Paciornik, Ester Fogel

    2014-01-01

    Professionalism in medicine requires preparation for the globalized world. Our objective was to describe a project that introduces medical students to the community, hospital and laboratory activities, thereby allowing them to gain experience in people management, leadership and teamwork. Descriptive study of the process applied at a philanthropic medical school in Curitiba, Paraná. Inclusion of management and leadership practices as part of the medical degree program. The study groups consisted of fifteen students. After six months, any of the participants could be elected as a subcoordinator, with responsibility for managing tasks and representing the team in hospital departments and the community. The activities required increasing levels of responsibility. In medical schools, students' involvement in practical activities is often limited to observation. They are not required to take responsibilities or to interact with other students and stakeholders. However, they will become accountable, which thus has an adverse effect on all involved. The learning space described here aims to fill this gap by bringing students closer to the daily lives and experiences of healthcare professionals. Being a physician requires not only management and leadership, but also transferrable competencies, communication and critical thinking. These attributes can be acquired through experience of teamwork, under qualified supervision from teaching staff. Students are thus expected to develop skills to deal with and resolve conflicts, learn to share leadership, prepare others to help and replace them, adopt an approach based on mutual responsibility and discuss their performance.

  13. Telmisartan prevention of LPS-induced microglia activation involves M2 microglia polarization via CaMKKβ-dependent AMPK activation.

    Science.gov (United States)

    Xu, Yuan; Xu, Yazhou; Wang, Yurong; Wang, Yunjie; He, Ling; Jiang, Zhenzhou; Huang, Zhangjian; Liao, Hong; Li, Jia; Saavedra, Juan M; Zhang, Luyong; Pang, Tao

    2015-11-01

    Brain inflammation plays an important role in the pathophysiology of many psychiatric and neurological diseases. During brain inflammation, microglia cells are activated, producing neurotoxic molecules and neurotrophic factors depending on their pro-inflammatory M1 and anti-inflammatory M2 phenotypes. It has been demonstrated that Angiotensin II type 1 receptor blockers (ARBs) ameliorate brain inflammation and reduce M1 microglia activation. The ARB telmisartan suppresses glutamate-induced upregulation of inflammatory genes in cultured primary neurons. We wished to clarify whether telmisartan, in addition, prevents microglia activation through polarization to an anti-inflammatory M2 phenotype. We found that telmisartan promoted M2 polarization and reduced M1 polarization in LPS-stimulated BV2 and primary microglia cells, effects partially dependent on PPARγ activation. The promoting effects of telmisartan on M2 polarization, were attenuated by an AMP-activated protein kinase (AMPK) inhibitor or AMPK knockdown, indicating that AMPK activation participates on telmisartan effects. Moreover, in LPS-stimulated BV2 cells, telmisartan enhancement of M2 gene expression was prevented by the inhibitor STO-609 and siRNA of calmodulin-dependent protein kinase kinase β (CaMKKβ), an upstream kinase of AMPK. Furthermore, telmisartan enhanced brain AMPK activation and M2 gene expression in a mouse model of LPS-induced neuroinflammation. In addition, telmisartan reduced the LPS-induced sickness behavior in this in vivo model, and this effect was prevented by prior administration of an AMPK inhibitor. Our results indicate that telmisartan can be considered as a novel AMPK activator, suppressing microglia activation by promoting M2 polarization. Telmisartan may provide a novel, safe therapeutic approach to treat brain disorders associated with enhanced inflammation.

  14. Involvement of CD244 in regulating CD4+ T cell immunity in patients with active tuberculosis.

    Directory of Open Access Journals (Sweden)

    Bingfen Yang

    Full Text Available CD244 (2B4 is a member of the signaling lymphocyte activation molecule (SLAM family of immune cell receptors and it plays an important role in modulating NK cell and CD8(+ T cell immunity. In this study, we investigated the expression and function of CD244/2B4 on CD4(+ T cells from active TB patients and latent infection individuals. Active TB patients had significantly elevated CD244/2B4 expression on M. tuberculosis antigen-specific CD4(+ T cells compared with latent infection individuals. The frequencies of CD244/2B4-expressing antigen-specific CD4(+ T cells were significantly higher in retreatment active TB patients than in new active TB patients. Compared with CD244/2B4-dull and -middle CD4(+ T cells, CD244/2B4-bright CD4(+ T cell subset had significantly reduced expression of IFN-γ, suggesting that CD244/2B4 expression may modulate IFN-γ production in M. tuberculosis antigen-responsive CD4(+ T cells. Activation of CD244/2B4 signaling by cross-linking led to significantly decreased production of IFN-γ. Blockage of CD244/2B4 signaling pathway of T cells from patients with active TB resulted in significantly increased production of IFN-γ, compared with isotype antibody control. In conclusion, CD244/2B4 signaling pathway has an inhibitory role on M. tuberculosis antigen-specific CD4(+ T cell function.

  15. Anticonvulsant activity of Citrus aurantium blossom essential oil (neroli): involvment of the GABAergic system.

    Science.gov (United States)

    Azanchi, Taravat; Shafaroodi, Hamed; Asgarpanah, Jinous

    2014-11-01

    Citrus aurantium L. blossoms are an important medicinal plant part in Iran and some other countries. It is used in traditional medicine as an antiseizure and anticonvulsant natural agent. Early in vitro research of the anticonvulsant activity of the blossom extracts were done but there has been no investigation focused on the blossom essential oil and its anticonvulsant activity. The anticonvulsant activity of the essential oil of C. aurantium blossoms (neroli) was investigated. The anticonvulsant activity of neroli was assessed in pentylenetetrazole (PTZ)-induced convulsion by i.v. and i.p. methods and maximal electroshock (MES) in mice, with diazepam as the standard drug. While mechanistic studies were conducted using flumazenil, a GABA A-benzodiazepine receptor complex site antagonist. Neroli produced protection against clonic by i.v adminiatration of PTZ at 20 and 40 mg/kg, compared with protection with benzodiazepine. The mean onset and percentage protection against convulsion in neroli-treated mice were reduced by flumazenil. Intraperitonaeal PTZ also decreased the latency of clonic seizure in the neroli (40 mg/kg) treated group. We also showed that neroli (20 and 40 mg/kg), exhibited inhibition of the tonic convulsion induced by MES and decreased the mortality rate. Neroli was analyzed by GC and GC-MS and twenty three constituents, representing 91.0 % of the chromatographical oil were identified. The major components of neroli were characterized as linalool (28.5%), linalyl acetate (19.6%), nerolidol (9.1%) E,E-farnesol (9.1%), α-terpineol (4.9%) and limonene (4.6%) which might be responsible for the anticonvulsant activity. The results suggest that neroli possesses biologically active constituent(s) that have anticonvulsant activity which supports the ethnomedicinal claims of the use of the plant in the management of seizure.

  16. No evidence for a putative involvement of platelet-activating factor in systemic lupus erythematosus without active nephritis

    Directory of Open Access Journals (Sweden)

    Yves Denizot

    2003-01-01

    Full Text Available Background: Platelet-activating factor (PAF seems to be implicated in systemic lupus erythematosus (SLE patients with associated renal diseases.Aims: In this study, we ensured the role of PAF in SLE patients without renal complications.

  17. Tumor cell alpha-N-acetylgalactosaminidase activity and its involvement in GcMAF-related macrophage activation.

    Science.gov (United States)

    Mohamad, Saharuddin B; Nagasawa, Hideko; Uto, Yoshihiro; Hori, Hitoshi

    2002-05-01

    Alpha-N-acetyl galactosaminidase (alpha-NaGalase) has been reported to accumulate in serum of cancer patients and be responsible for deglycosylation of Gc protein, which is a precursor of GcMAF-mediated macrophage activation cascade, finally leading to immunosuppression in advanced cancer patients. We studied the biochemical characterization of alpha-NaGalase from several human tumor cell lines. We also examined its effect on the potency of GcMAF to activate mouse peritoneal macrophage to produce superoxide in GcMAF-mediated macrophage activation cascade. The specific activity of alpha-NaGalases from human colon tumor cell line HCT116, human hepatoma cell line HepG2, and normal human liver cells (Chang liver cell line) were evaluated using two types of substrates; GalNAc-alpha-PNP (exo-type substrate) and Gal-beta-GalNAc-alpha-PNP (endo-type substrate). Tumor-derived alpha-NaGalase having higher activity than normal alpha-NaGalase, had higher substrate specificity to the exo-type substrate than to the endo-type substrate, and still maintained its activity at pH 7. GcMAF enhance superoxide production in mouse macrophage, and pre-treatment of GcMAF with tumor cell lysate reduce the activity. We conclude that tumor-derived alpha-NaGalase is different in biochemical characterization compared to normal alpha-NaGalase from normal Chang liver cells. In addition, tumor cell-derived alpha-NaGalase decreases the potency of GcMAF on macrophage activation.

  18. Participant and Public Involvement in Refining a Peer-Volunteering Active Aging Intervention: Project ACE (Active, Connected, Engaged).

    Science.gov (United States)

    Withall, Janet; Thompson, Janice L; Fox, Kenneth R; Davis, Mark; Gray, Selena; de Koning, Jolanthe; Lloyd, Liz; Parkhurst, Graham; Stathi, Afroditi

    2016-12-07

    Evidence for the health benefits of a physically active lifestyle among older adults is strong, yet only a small proportion of older people meet physical activity recommendations. A synthesis of evidence identified "best bet" approaches, and this study sought guidance from end-user representatives and stakeholders to refine one of these, a peer-volunteering active aging intervention. Focus groups with 28 older adults and four professional volunteer managers were conducted. Semi-structured interviews were conducted with 9 older volunteers. Framework analysis was used to gauge participants' views on the ACE intervention. Motives for engaging in community groups and activities were almost entirely social. Barriers to participation were lack of someone to attend with, lack of confidence, fear of exclusion or "cliquiness" in established groups, bad weather, transport issues, inaccessibility of activities, ambivalence, and older adults being "set in their ways". Motives for volunteering included "something to do," avoiding loneliness, the need to feel needed, enjoyment, and altruism. Challenges included negative events between volunteer and recipient of volunteering support, childcare commitments, and high volunteering workload. Peer-volunteering approaches have great potential for promotion of active aging. The systematic multistakeholder approach adopted in this study led to important refinements of the original ACE intervention. The findings provide guidance for active aging community initiatives highlighting the importance of effective recruitment strategies and of tackling major barriers including lack of motivation, confidence, and readiness to change; transport issues; security concerns and cost; activity availability; and lack of social support. © The Author 2016. Published by Oxford University Press on behalf of The Gerontological Society of America.

  19. Synchronized network activity in developing rat hippocampus involves regional hyperpolarization-activated cyclic nucleotide-gated (HCN) channel function

    OpenAIRE

    Bender, Roland A.; Galindo, Rafael; Mameli, Manuel; Gonzalez-Vega, Rebeca; Valenzuela, C. Fernando; Tallie Z. Baram

    2005-01-01

    The principal form of synchronized network activity in neonatal hippocampus consists of low frequency ‘giant depolarizing potentials’ (GDPs). Whereas contribution of both GABA and glutamate to their generation has been demonstrated, full understanding of the mechanisms underlying these synchronized activity bursts remains incomplete. A contribution of the h-current, conducted by HCN channels, to GDPs has been a topic of substantial interest. Here we focus on HCN1, the prevalent HCN channel is...

  20. IL-37 Confers Protection against Mycobacterial Infection Involving Suppressing Inflammation and Modulating T Cell Activation

    Science.gov (United States)

    Yang, Hua; He, Xin; Ji, Qun; Bai, Wenjuan; Chen, Hao; Chen, Jianxia; Peng, Wenxia; Liu, Siyu; Liu, Zhonghua; Ge, Baoxue

    2017-01-01

    Interleukin-37 (IL-37), a novel member of the IL-1 family, plays fundamental immunosuppressive roles by broadly reducing both innate inflammation and acquired immunity, but whether it is involved in the pathogenesis of tuberculosis (TB) has not been clearly elucidated. In this study, single nucleotide polymorphism (SNP) analysis demonstrated an association of the genetic variant rs3811047 of IL-37 with TB susceptibility. In line with previous report, a significant elevated IL-37 abundance in the sera and increased expression of IL-37 protein in the peripheral blood mononuclear cells (PBMC) were observed in TB patients in comparison to healthy controls. Moreover, release of IL-37 were detected in either macrophages infected with Mycobacterium tuberculosis (Mtb) or the lung of BCG-infected mice, concurrent with reduced production of proinflammatory cytokines including IL-6 and TNF-α. Furthermore, in contrast to wild-type mice, BCG-infected IL-37-Tg mice manifested with reduced mycobacterial burden and tissue damage in the lung, accompanied by higher frequency of Th1 cell and less frequencies of regulatory T cells and Th17 cells in the spleen. Taken together, our findings demonstrated that IL-37 conferred resistance to Mtb infection possibly involving suppressing detrimental inflammation and modulating T cell responses. These findings implicated that IL-37 may be employed as a new molecular target for the therapy and diagnosis of TB. PMID:28076390

  1. Clinical and psychological aspects of adolescent involvement in extremist and terrorist activities

    Directory of Open Access Journals (Sweden)

    Oshevsky D.S.

    2017-07-01

    Full Text Available The article deals with the clinical and psychological aspects of including minors in terrorist and extremist activities. In the historical perspective, it was traced how the views on the role of mental disorders in the genesis of such crimes changed. It is shown that terrorist and extremist activity must be viewed as a complex multi-factor phenomenon, in which socio-psychological components play a leading role. It is noted that the psychopathological process can act as a prerequisite for inclusion in such radical groups. Psychoanalytic, sociological, cognitive approaches, theories of social learning and the concept of diffuse ego-identity making attempts to explain the mechanisms of terrorist and extremist activity in minors are analyzed. The problem of insufficient study of the influence of the Internet and social networks on the formation of readiness for admission to adolescents in radical organizations is posed.

  2. Involvement of Activating NK Cell Receptors and Their Modulation in Pathogen Immunity

    Directory of Open Access Journals (Sweden)

    Francesco Marras

    2011-01-01

    Full Text Available Natural Killer (NK cells are endowed with cell-structure-sensing receptors providing inhibitory protection from self-destruction (inhibitory NK receptors, iNKRs, including killer inhibitory receptors and other molecules and rapid triggering potential leading to functional cell activation by Toll-like receptors (TLRs, cytokine receptors, and activating NK cell receptors including natural cytotoxicity receptors (NCRs, i.e., NKp46, NKp46, and NKp44. NCR and NKG2D recognize ligands on infected cells which may be endogenous or may directly bind to some structures derived from invading pathogens. In this paper, we address the known direct or indirect interactions between activating receptors and pathogens and their expression during chronic HIV and HCV infections.

  3. Facilitation handlings induce increase in electromyographic activity of muscles involved in head control of cerebral palsy children.

    Science.gov (United States)

    Simon, Anelise de Saldanha; do Pinho, Alexandre Severo; Grazziotin Dos Santos, Camila; Pagnussat, Aline de Souza

    2014-10-01

    This study aimed to investigate the electromyographic (EMG) activation of the main cervical muscles involved in the head control during two postures widely used for the facilitation of head control in children with Cerebral Palsy (CP). A crossover trial involving 31 children with clinical diagnosis of CP and spastic quadriplegia was conducted. Electromyography was used to measure muscular activity in randomized postures. Three positions were at rest: (a) lateral decubitus, (b) ventral decubitus on the floor and (c) ventral decubitus on the wedge. Handlings for facilitating the head control were performed using the hip joint as key point of control in two postures: (a) lateral decubitus and (b) ventral decubitus on wedge. All children underwent standardized handlings, performed by the same researcher with experience in the neurodevelopmental treatment. EMG signal was recorded from muscles involved in the head control (paraspinal and sternocleidomastoid muscles) in sagittal, frontal and transverse planes, at the fourth cervical vertebra (C4), tenth thoracic vertebra (T10) and sternocleidomastoid muscle (SCM) levels. The results showed a significant increase in muscle activation when handling was performed in the lateral decubitus at C4 (Pcontrol, as evaluated by the activity of cervical and upper trunk muscles. Handling performed in lateral decubitus may induce a slightly better facilitation of head control. These findings contribute to evidence-based physiotherapy practice for the rehabilitation of severely spastic quadriplegic CP children.

  4. Peripheral Neuropathy in the Twitcher Mouse Involves the Activation of Axonal Caspase 3

    Directory of Open Access Journals (Sweden)

    Benjamin Smith

    2011-09-01

    Full Text Available Infantile Krabbe disease results in the accumulation of lipid-raft-associated galactosylsphingosine (psychosine, demyelination, neurodegeneration and premature death. Recently, axonopathy has been depicted as a contributing factor in the progression of neurodegeneration in the Twitcher mouse, a bona fide mouse model of Krabbe disease. Analysis of the temporal-expression profile of MBP (myelin basic protein isoforms showed unexpected increases of the 14, 17 and 18.5 kDa isoforms in the sciatic nerve of 1-week-old Twitcher mice, suggesting an abnormal regulation of the myelination process during early postnatal life in this mutant. Our studies showed an elevated activation of the pro-apoptotic protease caspase 3 in sciatic nerves of 15- and 30-day-old Twitcher mice, in parallel with increasing demyelination. Interestingly, while active caspase 3 was clearly contained in peripheral axons at all ages, we found no evidence of caspase accumulation in the soma of corresponding mutant spinal cord motor neurons. Furthermore, active caspase 3 was found not only in unmyelinated axons, but also in myelinated axons of the mutant sciatic nerve. These results suggest that axonal caspase activation occurs before demyelination and following a dying-back pattern. Finally, we showed that psychosine was sufficient to activate caspase 3 in motor neuronal cells in vitro in the absence of myelinating glia. Taken together, these findings indicate that degenerating mechanisms actively and specifically mediate axonal dysfunction in Krabbe disease and support the idea that psychosine is a pathogenic sphingolipid sufficient to cause axonal defects independently of demyelination.

  5. Peripheral neuropathy in the Twitcher mouse involves the activation of axonal caspase 3

    Directory of Open Access Journals (Sweden)

    Ernesto R Bongarzone

    2011-10-01

    Full Text Available Infantile Krabbe disease results in the accumulation of lipid-raft-associated galactosylsphingosine (psychosine, demyelination, neurodegeneration and premature death. Recently, axonopathy has been depicted as a contributing factor in the progression of neurodegeneration in the Twitcher mouse, a bona fide mouse model of Krabbe disease. Analysis of the temporal-expression profile of MBP (myelin basic protein isoforms showed unexpected increases of the 14, 17 and 18.5 kDa isoforms in the sciatic nerve of 1-week-old Twitcher mice, suggesting an abnormal regulation of the myelination process during early postnatal life in this mutant. Our studies showed an elevated activation of the pro-apoptotic protease caspase 3 in sciatic nerves of 15- and 30-day-old Twitcher mice, in parallel with increasing demyelination. Interestingly, while active caspase 3 was clearly contained in peripheral axons at all ages, we found no evidence of caspase accumulation in the soma of corresponding mutant spinal cord motor neurons. Furthermore, active caspase 3 was found not only in unmyelinated axons, but also in myelinated axons of the mutant sciatic nerve. These results suggest that axonal caspase activation occurs before demyelination and following a dying-back pattern. Finally, we showed that psychosine was sufficient to activate caspase 3 in motor neuronal cells in vitro in the absence of myelinating glia. Taken together, these findings indicate that degenerating mechanisms actively and specifically mediate axonal dysfunction in Krabbe disease and support the idea that psychosine is a pathogenic sphingolipid sufficient to cause axonal defects independently of demyelination.

  6. Involving the public in general practice in an urban district: levels and type of activity and perceptions of obstacles.

    Science.gov (United States)

    Brown, Ian

    2000-07-01

    This paper reports on a study of the level and type of activity used to involve the public in general practice in a city district in the north of England. The association of these activities with features of the general practice organisation and environment were studied. Service providers' perceptions of obstacles were also studied. Data were collected in a survey of all general practice organisations in the district using a postal questionnaire completed by a practice manager. Interviews were conducted with health service managers responsible for primary care development in the district. The study showed that the district had a good track record for innovation in primary care development and in giving emphasis to developing public involvement. However, it also showed that it was difficult to translate policy rhetoric into practical initiatives at the general practice level without evidence of models of best practice, and with limited resources. The survey had a high response of over 84%. It showed that levels of activity were low across the district and only a small minority of general practice teams had undertaken a range of activities to involve the public. The socio-economic environment did not appear to be a factor, but small practices (one or two partners and/or practice population under 3000) were much less likely to develop activities. Pressures of existing workload, lack of resources and public apathy were given as among the main obstacles by survey respondents. The study indicates the challenges faced by Primary Care Groups in developing strands of public involvement. Primary care teams need a clear strategic framework, models of best practice, and adequate resources to manage, change and develop initiatives.

  7. Studies on the biotin-binding site of avidin. Tryptophan residues involved in the active site.

    Science.gov (United States)

    Gitlin, G; Bayer, E A; Wilchek, M

    1988-01-01

    Egg-white avidin was modified with the tryptophan-specific reagent 2-hydroxy-5-nitrobenzyl bromide. The complete loss of biotin-binding activity was achieved upon modification of an average of one tryptophan residue per avidin subunit. The identity of the modified residues was determined by isolating the relevant tryptic and chymotryptic peptides from CNBr-cleaved avidin fragments. The results demonstrate that Trp-70 and Trp-110 are modified in approximately equivalent proportions. It is believed that these residues are located in the active site of avidin and take part in the binding of biotin. PMID:3355517

  8. Studies on the biotin-binding site of avidin. Tryptophan residues involved in the active site.

    Science.gov (United States)

    Gitlin, G; Bayer, E A; Wilchek, M

    1988-02-15

    Egg-white avidin was modified with the tryptophan-specific reagent 2-hydroxy-5-nitrobenzyl bromide. The complete loss of biotin-binding activity was achieved upon modification of an average of one tryptophan residue per avidin subunit. The identity of the modified residues was determined by isolating the relevant tryptic and chymotryptic peptides from CNBr-cleaved avidin fragments. The results demonstrate that Trp-70 and Trp-110 are modified in approximately equivalent proportions. It is believed that these residues are located in the active site of avidin and take part in the binding of biotin.

  9. Use of Curricular and Extracurricular Assessments to Predict Performance on the United States Medical Licensing Examination (USMLE) Step 1: A Multi-Year Study

    Science.gov (United States)

    Gandy, Robyn A.; Herial, Nabeel A.; Khuder, Sadik A.; Metting, Patricia J.

    2008-01-01

    This paper studies student performance predictions based on the United States Medical Licensure Exam (USMLE) Step 1. Subjects were second-year medical students from academic years of 2002 through 2006 (n = 711). Three measures of basic science knowledge (two curricular and one extracurricular) were evaluated as predictors of USMLE Step 1 scores.…

  10. Online Mentoring as an Extracurricular Measure to Encourage Talented Girls in STEM (Science, Technology, Engineering, and Mathematics): An Empirical Study of One-on-One versus Group Mentoring

    Science.gov (United States)

    Stoeger, Heidrun; Hopp, Manuel; Ziegler, Albert

    2017-01-01

    Online mentoring provides an effective means of extracurricular gifted education for talented girls in science, technology, engineering, and mathematics (STEM). Comparative studies on the effectiveness of one-on-one versus group mentoring are lacking, however. The authors investigated this question in the context of a Germany-wide online mentoring…

  11. An After-School, high-intensity, interval physical activity programme improves health-related fitness in children

    Directory of Open Access Journals (Sweden)

    Sergio Reloba Martínez

    Full Text Available Abstract Health problems related to a low level of physical activity (PA in children and adolescents have prompted research into extracurricular PA programs. This study was designed to determine the effects of two different levels of PA on the health-related fitness of school children. Ninety-four girls and boys (7-9 years were randomly assigned to a control group (CG or intervention group (IG. Over a 12 week study period, children in the CG participated in a similar PA program to that of a standard school physical education program while those in the IG completed a high intensity interval training (HIIT program. Both programs involved two 40 minute extracurricular sessions per week. Our findings indicate that the HIIT intervention improved motor capacity (speed/agility, Vpeak, VO2 max and excess post-exercise oxygen consumption (EPOC (p < 0.05 along with the musculoskeletal capacity of the lower trunk (mean propulsive velocity and standing long jump, p < 0.05. The PA program had no effect on anthropometric variables or hand-grip strength. The data indicate that a 12 week strength training program using workloads adapted to children may significantly improve several markers of health and physical fitness compared to a standard school PA program.

  12. Involvement of the neuronal phosphotyrosine signal adaptor N-Shc in kainic acid-induced epileptiform activity.

    Science.gov (United States)

    Baba, Shiro; Onga, Kazuko; Kakizawa, Sho; Ohyama, Kyoji; Yasuda, Kunihiko; Otsubo, Hiroshi; Scott, Brian W; Burnham, W McIntyre; Matsuo, Takayuki; Nagata, Izumi; Mori, Nozomu

    2016-06-08

    BDNF-TrkB signaling is implicated in experimental seizures and epilepsy. However, the downstream signaling involved in the epileptiform activity caused by TrkB receptor activation is still unknown. The aim of the present study was to determine whether TrkB-mediated N-Shc signal transduction was involved in kainic acid (KA)-induced epileptiform activity. We investigated KA-induced behavioral seizures, epileptiform activities and neuronal cell loss in hippocampus between N-Shc deficient and control mice. There was a significant reduction in seizure severity and the frequency of epileptiform discharges in N-Shc deficient mice, as compared with wild-type and C57BL/6 mice. KA-induced neuronal cell loss in the CA3 of hippocampus was also inhibited in N-Shc deficient mice. This study demonstrates that the activation of N-Shc signaling pathway contributes to an acute KA-induced epileptiform activity and neuronal cell loss in the hippocampus. We propose that the N-Shc-mediated signaling pathway could provide a potential target for the novel therapeutic approaches of epilepsy.

  13. Peroxiredoxin-3 Is Involved in Bactericidal Activity through the Regulation of Mitochondrial Reactive Oxygen Species

    Science.gov (United States)

    Lee, Sena; Wi, Sae Mi; Min, Yoon

    2016-01-01

    Peroxiredoxin-3 (Prdx3) is a mitochondrial protein of the thioredoxin family of antioxidant peroxidases and is the principal peroxidase responsible for metabolizing mitochondrial hydrogen peroxide. Recent reports have shown that mitochondrial reactive oxygen species (mROS) contribute to macrophage-mediated bactericidal activity in response to Toll-like receptors. Herein, we investigated the functional effect of Prdx3 in bactericidal activity. The mitochondrial localization of Prdx3 in HEK293T cells was confirmed by cell fractionation and confocal microscopy analyses. To investigate the functional role of Prdx3 in bactericidal activity, Prdx3-knockdown (Prdx3KD) THP-1 cells were generated. The mROS levels in Prdx3KD THP-1 cells were significantly higher than those in control THP-1 cells. Moreover, the mROS levels were markedly increased in response to lipopolysaccharide. Notably, the Salmonella enterica serovar Typhimurium infection assay revealed that the Prdx3KD THP-1 cells were significantly resistant to S. Typhimurium infection, as compared with control THP-1 cells. Taken together, these results indicate that Prdx3 is functionally important in bactericidal activity through the regulation of mROS. PMID:28035213

  14. The ability of thapsigargin and thapsigargicin to activate cells involved in the inflammatory response

    DEFF Research Database (Denmark)

    Ali, H; Christensen, S B; Foreman, J C;

    1985-01-01

    The ability of thapsigargin and thapsigargicin to activate mast cells and leukocytes has been investigated. The thapsigargin-induced histamine release from rat peritoneal mast cells was found to be dependent on the concentration of thapsigargin, the purity of the mast cell preparations, and the n...

  15. Fantasy Activity and the Televiewing Event: Considerations for an Information Processing Construct of Involvement.

    Science.gov (United States)

    Lindlof, Thomas R.

    The similarities between television viewing and fantasy activity (daydreaming, reverie, mind-wandering, internal dialogue) more than warrant the building of a theoretical construct, especially in the context of recent empirical research on television viewing consequences. A construct of the television viewing process, based on cognitive theories…

  16. Listening and Speaking to Help Students Become Involved Listeners and Active Speakers

    Institute of Scientific and Technical Information of China (English)

    DingAiyun

    2004-01-01

    This paper intends to reveal the potential obstacles that may hinder students from understanding a listening text, to discuss the right procedures and techniques that a teacher should adopt in class and to suggest some post-listening activities that can be turned into oral practice.

  17. A Multi-Institutional Examination of the Relationships between High School Activity Involvement and Leadership Characteristics

    Science.gov (United States)

    Simonsen, Jon C.; Velez, Jonathan J.; Foor, Ryan M.; Birkenholz, Robert J.; Foster, Daniel D.; Wolf, Kattlyn J.; Epps, Rebekah B.

    2014-01-01

    Developing leaders is vital to the future of our nation. As institutions of higher education address the need for leadership through courses and experiences, there is a need to recognize and acknowledge the potential impact of high school activity participation on leadership development. This descriptive-correlational study surveyed first-time…

  18. [Activity involvement and extraversion as predictors of psychological wellbeing in older people].

    Science.gov (United States)

    Rubio, Laura; Dumitrache, Cristina G; Rubio-Herrera, Ramona

    2016-01-01

    The relationship between extraversion and wellbeing has been discussed in the literature, however, the impact that this trait has on the wellbeing of older people has been studied to a lesser extent. The relationship between extraversion, participation in activities and psychological wellbeing in older people is analysed in this study. The sample comprised 139 individuals over 55 years from rural and urban areas of the province of Granada who completed the extraversion subscale of the NEO-FFI and the Ryff Scales of the Psychological Wellbeing, as well as responding to questions that evaluated their social participation. A greater social participation was found in rural areas and among women. The activities more frequently performed by the participants were educational and religious activities, walking, everyday chores, crafts, and home improvements. A low positive correlation between extraversion and wellbeing was observed. The multiple regression analysis revealed that extraversion explained 19.9% of the variance in psychological wellbeing, which increased to 25.3% when social participation, gender, and the origin of the sample were considered. Psychological wellbeing appears to be associated with personality traits, such as extraversion. In addition this personality trait is linked to the number and type of activities the elderly perform which also contributes to wellbeing in old age. Copyright © 2015 SEGG. Published by Elsevier Espana. All rights reserved.

  19. Families and School Personnel Involved in a Literacy and Physical Activity Partnership

    Science.gov (United States)

    Richardson, James A.; Richardson, Maurine V.; Sacks, Mary Kathleen

    2006-01-01

    A traditional part of American education has been to include the families in the educational process of their children. The needs and complexities of today's families and classrooms have never been greater. Programs and activities used with families, and school interactions 10 to 15 years ago are not effective for today's complex families and…

  20. GBA2-encoded β-glucosidase activity is involved in the inflammatory response to Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Nicoletta Loberto

    Full Text Available Current anti-inflammatory strategies for the treatment of pulmonary disease in cystic fibrosis (CF are limited; thus, there is continued interest in identifying additional molecular targets for therapeutic intervention. Given the emerging role of sphingolipids (SLs in various respiratory disorders, including CF, drugs that selectively target the enzymes associated with SL metabolism are under development. Miglustat, a well-characterized iminosugar-based inhibitor of β-glucosidase 2 (GBA2, has shown promise in CF treatment because it reduces the inflammatory response to infection by P. aeruginosa and restores F508del-CFTR chloride channel activity. This study aimed to probe the molecular basis for the anti-inflammatory activity of miglustat by examining specifically the role of GBA2 following the infection of CF bronchial epithelial cells by P. aeruginosa. We also report the anti-inflammatory activity of another potent inhibitor of GBA2 activity, namely N-(5-adamantane-1-yl-methoxypentyl-deoxynojirimycin (Genz-529648. In CF bronchial cells, inhibition of GBA2 by miglustat or Genz-529648 significantly reduced the induction of IL-8 mRNA levels and protein release following infection by P. aeruginosa. Hence, the present data demonstrate that the anti-inflammatory effects of miglustat and Genz-529648 are likely exerted through inhibition of GBA2.

  1. Dexamethasone enhances necrosis-like neuronal death in ischemic rat hippocampus involving μ-calpain activation

    DEFF Research Database (Denmark)

    Müller, Georg Johannes; Hasseldam, Henrik; Rasmussen, Rune Skovgaard;

    2014-01-01

    Transient forebrain ischemia (TFI) leads to hippocampal CA1 pyramidal cell death which is aggravated by glucocorticoids (GC). It is unknown how GC affect apoptosis and necrosis in cerebral ischemia. We therefore investigated the co-localization of activated caspase-3 (casp-3) with apoptosis- and ...

  2. Activation of hepatic lipase expression by oleic acid: possible involvement of USF1.

    NARCIS (Netherlands)

    D. van Deursen (Diederik); M. van Leeuwen (Marije); D. Akdogan (Deniz); H. Adams (Hadie); H. Jansen (Hans); A.J.M. Verhoeven (Adrie)

    2009-01-01

    textabstractPolyunsaturated fatty acids affect gene expression mainly through peroxisome proliferator-activated receptors (PPARs) and sterol regulatory element binding proteins (SREBPs), but how monounsaturated fatty acids affect gene expression is poorly understood. In HepG2 cells, oleate supplemen

  3. The Development of Spatial Skills through Interventions Involving Block Building Activities

    Science.gov (United States)

    Casey, Beth M.; Andrews, Nicole; Schindler, Holly; Kersh, Joanne E.; Samper, Alexandra; Copley, Juanita

    2008-01-01

    This study investigated the use of block-building interventions to develop spatial-reasoning skills in kindergartners. Two intervention conditions and a control condition were included to determine, first, whether the block building activities themselves benefited children's spatial skills, and secondly, whether a story context further improved…

  4. In vitro activity of aminosterols against yeasts involved in blood stream infections.

    Science.gov (United States)

    Alhanout, Kamel; Djouhri, Lamia; Vidal, Nicolas; Brunel, Jean Michel; Piarroux, Renaud; Ranque, Stéphane

    2011-02-01

    Squalamine and other aminosterols have demonstrated interesting antimicrobial activities against clinical bacterial isolates and a limited number of reference yeast strains. We aimed to test whether squalamine and a synthetic aminosterol derivative (ASD) display any in vitro activity comparable to currently available systemic antifungals, an acceptable safety index, as well as to provide insights into their mechanism of action. The minimum inhibitory concentrations (MICs) of squalamine, ASD and available antifungals were determined against 21 yeast isolates that were recovered from cases of fungemia. Remarkably, homogeneous MICs ranging from 8-16 mg/L and from 1-2 mg/L were noted for squalamine and ASD, respectively, as opposes the heterogeneous in vitro activity of available systemic antifungals. Aminosterols induced haemolysis, a surrogate for toxic effects to mammalian cells, at concentrations high above their MICs. In time-kill studies, killing was as fast with ASD as with amphotericin B. Both aminosterols induced a time-dependent disruption of yeast membrane, as evidenced by gradual increase of ATP efflux. In conclusion, our preliminary data indicate that aminosterols have the potential to be further developed as antifungals. Additional work is warranted to assess their toxicity and activity in experimental models.

  5. Ethanol Effects Involve Non-canonical Unfolded Protein Response Activation in Yeast Cells

    Science.gov (United States)

    Navarro-Tapia, Elisabet; Pérez-Torrado, Roberto; Querol, Amparo

    2017-01-01

    The unfolded protein response (UPR) is a conserved intracellular signaling pathway that controls transcription of endoplasmic reticulum (ER) homeostasis related genes. Ethanol stress has been recently described as an activator of the UPR response in yeast Saccharomyces cerevisiae, but very little is known about the causes of this activation. Although some authors ensure that the UPR is triggered by the unfolded proteins generated by ethanol in the cell, there are studies which demonstrate that protein denaturation occurs at higher ethanol concentrations than those used to trigger the UPR. Here, we studied UPR after ethanol stress by three different approaches and we concluded that unfolded proteins do not accumulate in the ER under. We also ruled out inositol depletion as an alternative mechanism to activate the UPR under ethanol stress discarding that ethanol effects on the cell decreased inositol levels by different methods. All these data suggest that ethanol, at relatively low concentrations, does not cause unfolded proteins in the yeasts and UPR activation is likely due to other unknown mechanism related with a restructuring of ER membrane due to the effect of ethanol. PMID:28326077

  6. The amrG1 gene is involved in the activation of acetate in Corynebacterium glutamicum

    Institute of Scientific and Technical Information of China (English)

    RUAN; Hong; R.; Gerstmeir; S.; Schnicke; B.J.; Eikmanns

    2005-01-01

    During growth of Corynebacterium glutamicum on acetate as its carbon and energy source, the expression of the pta-ack operon is induced, coding for the acetate-activating enzymes, which are phosphotransacetylase (PTA) and acetate kinase (AK). By transposon rescue, we identified the two genes amrG1 and amrG2 found in the deregulated transposon mutant C. glutamicum G25. The amrG1 gene (NCBI-accession: AF532964) has a size of 732 bp, encoding a polypeptide of 243 amino acids and apparently is partially responsible for the regulation of acetate metabolism in C. glutamicum. We constructed an in-frame deletion mutant and an overexpressing strain of amrG1 in the C. glutamicum ATCC13032 wildtype. The strains were then analyzed with respect to their enzyme activities of PTA and AK during growth on glucose, acetate and glucose or acetate alone as carbon sources. Compared to the parental strain, the amrG1 deletion mutant showed higher specific AK and PTA activities during growth on glucose but showed the same high specific activities of AK and PTA on medium containing acetate plus glucose and on medium containing acetate. In contrast to the gene deletion, overexpression of the amrG1 gene in C. glutamicum 13032 had the adverse regulatory effect. These results indicate that the amrG1 gene encodes a repressor or co-repressor of the pta-ack operon.

  7. Marketing Informal Education Institutions in Israel: The Centrality of Customers' Active Involvement in Service Development

    Science.gov (United States)

    Oplatka, Izhar

    2004-01-01

    The current paper outlines a unique marketing perspective that prevails in some informal education institutions in Israel parallel with "traditional modes of marketing", such as promotion, public relations and the like. Based on a case study research in five community centres, a service development based on active participation of the…

  8. Everyday Curators: Collecting as Literate Activity

    Science.gov (United States)

    Rohan, Liz

    2010-01-01

    In Mary Louise Pratt's oft-cited essay, "Arts of the Contact Zone," she argues that her son Sam's extracurricular hobby as a baseball card collector taught him about economics, racism, and American history, constituting literate activity that enabled him to hold his own in conversations with adults. Sam was also playing baseball at the time in…

  9. Everyday Curators: Collecting as Literate Activity

    Science.gov (United States)

    Rohan, Liz

    2010-01-01

    In Mary Louise Pratt's oft-cited essay, "Arts of the Contact Zone," she argues that her son Sam's extracurricular hobby as a baseball card collector taught him about economics, racism, and American history, constituting literate activity that enabled him to hold his own in conversations with adults. Sam was also playing baseball at the time in…

  10. Dopamine receptor-mediated mechanisms involved in the expression of learned activity of primate striatal neurons.

    Science.gov (United States)

    Watanabe, K; Kimura, M

    1998-05-01

    To understand the mechanisms by which basal ganglia neurons express acquired activities during and after behavioral learning, selective dopamine (DA) receptor antagonists were applied while recording the activity of striatal neurons in monkeys performing behavioral tasks. In experiment 1, a monkey was trained to associate a click sound with a drop of reward water. DA receptor antagonists were administered by micropressure using a stainless steel injection cannula (300 microm ID) through which a Teflon-coated tungsten wire for recording neuronal activity had been threaded. Responses to sound by tonically active neurons (TANs), a class of neurons in the primate striatum, were recorded through a tungsten wire electrode during the application of either D1- or D2-class DA receptor antagonists (total volume one of the surrounding barrels. SCH23390 (10 mM, pH 4.5) and (-)-sulpiride (10 mM, pH 4.5) were used. The effects of iontophoresis of both D1- and D2-class antagonists were examined in 40 TANs. Of 40 TANs from which recordings were made, responses were suppressed exclusively by the D2-class antagonist in 19 TANs, exclusively by the D1-class antagonist in 3 TANs, and by both D1- and D2-class antagonists in 7 TANs. When 0.9% NaCl, saline, was applied by pressure (<1 microl) or by iontophoresis (<30 nA) as a control, neither the background discharge rates nor the responses of TANs were significantly influenced. Background discharge rate of TANs was also not affected by D1- or D2-class antagonists applied by either micropressure injection or iontophoresis. It was concluded that the nigrostriatal DA system enables TANs to express learned activity primarily through D2-class and partly through D1-class receptor-mediated mechanisms in the striatum.

  11. Oncogene activation in human benign tumors of the skin (keratoacanthomas): Is HRAS involved in differentiation as well as proliferation

    Energy Technology Data Exchange (ETDEWEB)

    Corominas, M.; Kamino, Hideko; Leon, J.; Pellicer, A. (New York Univ. Medical Center, New York, NY (USA))

    1989-08-01

    In vitro DNA amplification followed by oligonucleotide mismatch hybridization was used to study the frequency of HRAS mutations in the benign self-regressing skin tumors keratoacanthomas and in squamous cell carcinomas. The authors used freshly obtained keratoacanthomas as well as Formalin-fixed paraffin-embedded tissues from both types of tumors. DNA from 50 samples of each tumor type was analyzed for activating mutations involving codons 12 and 61. A relatively high percentage (30%) of HRAS mutations was found in the keratoacanthomas compared with 13% in the squamous cell carcinomas. The most frequent mutation identified is the A{center dot}T-to-T{center dot}A transversion in the second position of codon 61. The present findings demonstrate the involvement of the HRAS oncogene in human benign tumors. Moreover, they indicate that an activated HRAS oncogene is not sufficient to maintain a neoplastic phenotype and argue against a role of HRAS in the progression of skin tumorigenesis.

  12. Involvement of activation of NADPH oxidase and extracellular signal-regulated kinase (ERK) in renal cell injury induced by zinc.

    Science.gov (United States)

    Matsunaga, Yoshiko; Kawai, Yoshiko; Kohda, Yuka; Gemba, Munekazu

    2005-05-01

    Zinc is employed as a supplement; however, zinc-related nephropathy is not generally known. In this study, we investigated zinc-induced renal cell injury using a pig kidney-derived cultured renal epithelial cell line, LLC-PK(1), with proximal kidney tubule-like features, and examined the involvement of free radicals and extracellular signal-regulated kinase (ERK) in the cell injury. The LLC-PK(1) cells showed early uptake of zinc (30 microM), and the release of lactate dehydrogenase (LDH), an index of cell injury, was observed 24 hr after uptake. Three hours after zinc exposure, generation of reactive oxygen species (ROS) was increased. An antioxidant, N, N'-diphenyl-p-phenylenediamine (DPPD), inhibited a zinc-related increase in ROS generation and zinc-induced renal cell injury. An NADPH oxidase inhibitor, diphenyleneiodonium (DPI), inhibited a zinc-related increase in ROS generation and cell injury. We investigated translocation from the cytosol fraction of the p67(phox) subunit, which is involved in the activation of NADPH oxidase, to the membrane fraction, and translocation was induced 3 hr after zinc exposure. We examined the involvement of ERK1/2 in the deterioration of zinc-induced renal cell injury, and the association between ERK1/2 and an increase in ROS generation. Six hours after zinc exposure, the activation (phosphorylation) of ERK1/2 was observed. An antioxidant, DPPD, inhibited the zinc-related activation of ERK1/2. An MAPK/ERK kinase (MEK1/2) inhibitor, U0126, almost completely inhibited zinc-related cell injury (the release of LDH), but did not influence ROS generation. These results suggest that early intracellular uptake of zinc by LLC-PK(1) cells causes the activation of NADPH oxidase, and that ROS generation by the activation of the enzyme leads to the deterioration of renal cell injury via the activation of ERK1/2.

  13. QUANTITATIVE STUDY ON THE INVOLVEMENT OF BRICOLAGE COMPANIES IN SOCIAL RESPONSIBILITY ACTIVITIES

    Directory of Open Access Journals (Sweden)

    Mihai-Cosmin FANARU

    2016-07-01

    Full Text Available The main purpose of this paper is to analyze the influence of social responsibility actions of the main bricolage companies doing business in Romania on their value, and mutually the impact these activities have on various social sectors. CSR (Corporate Social Responsibility is a concept related to the contribution that companies need to have to the development of modern society. Over time, this contribution has been theorized by many different schools of thought. "Responsible" initiatives of companies have been named by a variety of terms: corporate citizenship, corporate philanthropy, corporate societal marketing, community affairs, community development etc. Consequently, currently, to demonstrate that it is "socially responsible", a company must understand the principles of CSR that are internationally promoted and regularly report about the integration of these principles in its activities.

  14. QUANTITATIVE STUDY ON THE INVOLVEMENT OF BRICOLAGE COMPANIES IN SOCIAL RESPONSIBILITY ACTIVITIES

    Directory of Open Access Journals (Sweden)

    Mihai-Cosmin FANARU

    2016-07-01

    Full Text Available The main purpose of this paper is to analyze the influence of social responsibility actions of the main bricolage companies doing business in Romania on their value, and mutually the impact these activities have on various social sectors. CSR (Corporate Social Responsibility is a concept related to the contribution that companies need to have to the development of modern society. Over time, this contribution has been theorized by many different schools of thought. "Responsible" initiatives of companies have been named by a variety of terms: corporate citizenship, corporate philanthropy, corporate societal marketing, community affairs, community development etc. Consequently, currently, to demonstrate that it is "socially responsible", a company must understand the principles of CSR that are internationally promoted and regularly report about the integration of these principles in its activities.

  15. Isolation of a gene that is involved in Campylobacter jejuni 81116 cytotoxin activation.

    Science.gov (United States)

    Liu, Kaiyan; Fry, Benjamin N; Coloe, Peter J

    2007-02-01

    Cytotoxin fractions were isolated from Campylobacter jejuni 81116 and semi-purified by size-exclusion liquid chromatography. The fraction showing the strongest toxicity was injected into mice to produce antiserum. The antiserum was used to screen a C. jejuni 81116 cosmid library. Nine genes were identified in overlapping cosmid inserts that induced reactivity with the antiserum. One of these genes showed high similarity to a periplasmic protein of unknown function and its isogenic mutant showed decreased toxicity compared to the C. jejuni 81116 wild type. This gene contains a Gram-negative bacterial RTX toxin-activating protein C signature, which suggests it may play a role in C. jejuni 81116 cytotoxin activation.

  16. Sexual attitudes and activities in women with borderline personality disorder involved in romantic relationships.

    Science.gov (United States)

    Bouchard, Sébastien; Godbout, Natacha; Sabourin, Stéphane

    2009-01-01

    Women with Borderline Personality Disorder (BPD) are prone to have sexual relationship difficulties and dysfunctional attitudes toward sexuality. A sample of 34 heterosexual couples composed of women meeting BPD criteria was compared to a sample of dating or married women from the general population. A short form of the Sexual Activities and Attitudes Questionnaire (SAAQ) was used to measure six types of sexual attitudes. Women diagnosed with BPD did not differ from controls on the frequency of three types of sexual activities in the last year but their subjective sexual experiences differed: they showed stronger negative attitudes, felt sexually pressured by their partners, and expressed ambivalence toward sexuality. Regression analyses suggest that anxious attachment mediates the association between BPD and feeling pressured to engage in sex.

  17. Factors involved in the regulation of a ligninase activity in Phanerochaete chrysosporium

    Energy Technology Data Exchange (ETDEWEB)

    Faison, B.D.; Kikr, T.K.

    1985-02-01

    The regulation of an H/sub 2/O/sub 2/-dependent ligninolytic activity was examined in the wood decay fungus Phanerochaete chrysosporium. The ligninase appears in cultures upon limitation of nitrogen or carbohydrate and is suppressed by excess nutrients, by cycloheximide, or by culture agitation. Activity is increased by idiophasic exposure of cultures to 100% O/sub 2/. Elevated levels of ligninase and in some cases, of extracellular H/sub 2/O/sub 2/ production are detected after brief incubation of cultures with lignins or lignin substructure models, with the secondary metabolite veratryl alcohol, or with other related compounds. It is concluded that lignin degradation (lignin to CO/sub 2/) by this organism is regulated in part at the level of the ligninase, which is apparently inducible by its substrates or their degradation products. 29 references.

  18. 'Co-active coaching' could help HIV patients. New type of counseling involves goal-setting.

    Science.gov (United States)

    Garfinkel, M; Blumenthal, E

    2001-08-01

    A counseling technique that takes an action-oriented approach to helping people make major life changes, much used by business executives and other professionals in recent years, now appears to offer some value to HIV patients. Co-active coaching could be a solution to mild depression and inertia for some HIV-infected patients who have difficulty making decisions about how to spend a life-time living with the disease.

  19. Involvements of chloride ion in decolorization of Acid Orange 7 by activated peroxydisulfate or peroxymonosulfate oxidation

    Institute of Scientific and Technical Information of China (English)

    Ping Wang; Shiying Yang; Liang Shan; Rui Niu; Xueting Shao

    2011-01-01

    The effects of chloride anion (C1-) (up to 1.0 mol/L) on the decolorization of a model compound,azo dye Acid Orange 7 (AO7),by sulfate radical (SO4-*) based-peroxydisulfate (PS) or peroxymonosulfate (PMS) oxidation under various activated conditions (UV254 nm/PS,Thermal (70℃/PS,UV254 nm/PMS,Co2+/PMS) were investigated.Methanol and NH4+ were used as quenching reagents to determine the contributions of active chlorine species (dichloride radical (Cl2-*) and hypochlorous acid (HClO)).The results indicated that the effects of Cl- on the reaction mechanism were different under various activated conditions.For UV/PS and Thermal/PS,the inhibition tendency became more clear as the Cl-concentration increased,probably due to the reaction between Cl- and SO4-* and the generation of Cl2-* or HClO.For UV/PMS,Cl- did not exhibit inhibition when the concentration was below 0.1 mol/L.As Cl-concentration reached to 1.0 mol/L,the decolorization rate of AO7 was,however,accelerated,possibly because PMS directly reacts with C1- to form HC1O.For Co2+/PMS,Cl- exhibited a significant inhibiting effect even at low concentration (≤ 0.01 mol/L).When Cl- concentration exceeded 0.1 mol/L,the activation of PMS by Co2+ was almost completely inhibited.Under this condition,HClO maybe played a major role in decolorization of AO7.The results implicated that chloride ion is an important factor in SO4-*-based degradation of organic contamination in chloride-containing water.

  20. Studies on the biotin-binding site of avidin. Tryptophan residues involved in the active site.

    OpenAIRE

    Gitlin, G; Bayer, E A; Wilchek, M

    1988-01-01

    Egg-white avidin was modified with the tryptophan-specific reagent 2-hydroxy-5-nitrobenzyl bromide. The complete loss of biotin-binding activity was achieved upon modification of an average of one tryptophan residue per avidin subunit. The identity of the modified residues was determined by isolating the relevant tryptic and chymotryptic peptides from CNBr-cleaved avidin fragments. The results demonstrate that Trp-70 and Trp-110 are modified in approximately equivalent proportions. It is beli...

  1. Effects of three types of physical activity on reduction of metabolic parameters involved in cardiovascular risk

    OpenAIRE

    Petrović-Oggiano Gordana; Damjanov Vlasta; Vučić Vesna; Debeljak-Martačić Jasmina; Pavlović Mirjana; Glibetić Marija

    2009-01-01

    The aim of present study was to investigate the effects of three different types of physical activity on reduction of the metabolic parameters mainly responsible for cardiovascular diseases. This prospective-intervention study was performed at the 'ČIGOTA' Thyroid Institute on Mt. Zlatibor (Serbia) between August 2004 and June 2006. Sixty-eight overweight/obese patients aged 40-70 years with hyperlipidemia were divided into three groups according to their weight and overall health. The progra...

  2. Administrator Interest is Perceived to Encourage Faculty and Librarian Involvement in Open Access Activities

    OpenAIRE

    Eamon C. Tewell

    2014-01-01

    A Review of: Reinsfelder, T.L., & Anderson, J.A. (2013). Observations and perceptions of academic administrator influence on open access initiatives. Journal of Academic Librarianship, 39(6): 481-487. http://dx.doi.org/10.1016/j.acalib.2013.08.014 Abstract Objective – To better understand the roles and influence of senior-level academic administrators, such as provosts, on open access (OA) activities at the institutional level, including whether librarians perform these activit...

  3. Estrogen receptor-mediated transcription involves the activation of multiple kinase pathways in neuroblastoma cells.

    Science.gov (United States)

    Clark, Sara; Rainville, Jennifer; Zhao, Xing; Katzenellenbogen, Benita S; Pfaff, Donald; Vasudevan, Nandini

    2014-01-01

    While many physiological effects of estrogens (E) are due to regulation of gene transcription by liganded estrogen receptors (ERs), several effects are also mediated, at least in part, by rapid non-genomic actions of E. Though the relative importance of rapid versus genomic effects in the central nervous system is controversial, we showed previously that membrane-limited effects of E, initiated by an estradiol bovine serum albumin conjugate (E2-BSA), could potentiate transcriptional effects of 17β-estradiol from an estrogen response element (ERE)-reporter in neuroblastoma cells. Here, using specific inhibitors and activators in a pharmacological approach, we show that activation of phosphatidylinositol-3-phosphate kinase (PI3K) and mitogen activated protein kinase (MAPK) pathways, dependent on a Gαq coupled receptor signaling are important in this transcriptional potentiation. We further demonstrate, using ERα phospho-deficient mutants, that E2-BSA mediated phosphorylation of ERα is one mechanism to potentiate transcription from an ERE reporter construct. This study provides a possible mechanism by which signaling from the membrane is coupled to transcription in the nucleus, providing an integrated view of hormone signaling in the brain.

  4. Involvement of urokinase-type plasminogen activator system in cancer: an overview.

    Science.gov (United States)

    Mekkawy, Ahmed H; Pourgholami, Mohammad H; Morris, David L

    2014-09-01

    Currently, there are several studies supporting the role of urokinase-type plasminogen activator (uPA) system in cancer. The association of uPA to its receptor triggers the conversion of plasminogen into plasmin. This process is regulated by the uPA inhibitors (PAI-1 and PAI-2). Plasmin promotes degradation of basement membrane and extracellular matrix (ECM) components as well as activation of ECM latent matrix metalloproteases. Degradation and remodeling of the surrounding tissues is crucial in the early steps of tumor progression by facilitating expansion of the tumor mass, release of tumor growth factors, activation of cytokines as well as induction of tumor cell proliferation, migration, and invasion. Hence, many tumors showed a correlation between uPA system component levels and tumor aggressiveness and survival. Therefore, this review summarizes the structure of the uPA system, its contribution to cancer progression, and the clinical relevance of uPA family members in cancer diagnosis. In addition, the review evaluates the significance of uPA system in the development of cancer-targeted therapies.

  5. Involvement of Activated Cdc42 Kinase1 in Colitis and Colorectal Neoplasms.

    Science.gov (United States)

    Lv, Chaolan; Zhao, Xinmei; Gu, Hongxiang; Huang, Liyun; Zhou, Sanxi; Zhi, Fachao

    2016-12-07

    BACKGROUND Activated Cdc42 kinase1 (ACK1) is a non-receptor tyrosine kinase which is critical for cell survival, proliferation, and migration. Genomic amplification of ACK1 has been reported in multiple human cancers. We aimed to investigate ACK1 protein expression in colorectal mucosa with inflammation and neoplasm, and to evaluate its correlation with disease activity and severity. MATERIAL AND METHODS A total of 250 individuals who underwent total colonoscopy were collected randomly from January 2007 to May 2013 in Nanfang Hospital, Guangzhou, China. Colorectal mucosal biopsy specimens were obtained by endoscopy from 78 patients with ulcerative colitis (UC), 22 with Crohn's disease (CD), 20 with infectious colitis, 26 with non-IBD and noninfectious colitis, 16 with sporadic adenomas, 4 with dysplasia-associated lesions or masses, 10 with sporadic colorectal cancer (CRC), 4 with UC-related CRC, 10 with hyperplastic polyps, and 60 without colonic abnormalities. ACK1 protein levels were determined immunohistochemically. The correlations of ACK1 expression with disease activity and severity were also evaluated. RESULTS Significantly increased ACK1 expression was observed in epithelial cells of colorectal mucosa with inflammation and dysplasia compared to controls (P0.05). CONCLUSIONS ACK1 protein is increased extensively in colitis and colorectal dysplasia. ACK1 overexpression may play a role in colorectal inflammation and neoplasms.

  6. Production of lysophosphatidic acid in blister fluid: involvement of a lysophospholipase D activity.

    Science.gov (United States)

    Mazereeuw-Hautier, Juliette; Gres, Sandra; Fanguin, Madie; Cariven, Clotilde; Fauvel, Josette; Perret, Bertrand; Chap, Hugues; Salles, Jean-Pierre; Saulnier-Blache, Jean-Sébastien

    2005-09-01

    Lysophosphatidic acid (LPA) is present in abundance in serum resulting from platelet activation and is also found in other biological fluids. LPA controls numerous cellular responses and plays a role in specific functions such as wound healing, especially in the skin. Nevertheless, its presence in the skin has never been investigated. Since re-epithelialization occurs after blister rupture, we tested the presence of endogenous LPA in blister fluid and investigated a possible mechanism for its biosynthesis and biological functions. Using a radioenzymatic assay, LPA was detected in 33 blister fluids originating from 24 bullous dermatoses, and at higher concentrations than in plasma. In parallel, blister fluids contained a lysophospholipase D (LPLD) activity but no detectable phospholipase A2 activity. The expressions of the LPLD autotaxin (ATX) and of LPA1-receptor (LPA1-R) were greatly increased in blister skin when compared with normal skin. Finally, LPA was found to have a positive effect on the migration of cultured keratinocytes. These results show that LPA is present in blister fluid synthesized by the LPLD ATX. Due to its ability to enhance keratinocyte migration, LPA in blister fluid could, via the LPA1-R, play an important role in re-epithelialization occurring after blister rupture.

  7. Involvement of Fungal Pectin Methylesterase Activity in the Interaction Between Fusarium graminearum and Wheat.

    Science.gov (United States)

    Sella, Luca; Castiglioni, Carla; Paccanaro, Maria Chiara; Janni, Michela; Schäfer, Wilhelm; D'Ovidio, Renato; Favaron, Francesco

    2016-04-01

    The genome of Fusarium graminearum, the causal agent of Fusarium head blight of wheat, contains two putative pectin methylesterase (PME)-encoding genes. However, when grown in liquid culture containing pectin, F. graminearum produces only a single PME, which was purified and identified. Its encoding gene, expressed during wheat spike infection, was disrupted by targeted homologous recombination. Two Δpme mutant strains lacked PME activity but were still able to grow on highly methyl-esterified pectin even though their polygalacturonase (PG) activity showed a reduced capacity to depolymerize this substrate. The enzymatic assays performed with purified F. graminearum PG and PME demonstrated an increase in PG activity in the presence of PME on highly methyl-esterified pectin. The virulence of the mutant strains was tested on Triticum aestivum and Triticum durum spikes, and a significant reduction in the percentage of symptomatic spikelets was observed between 7 and 12 days postinfection compared with wild type, demonstrating that the F. graminearum PME contributes to fungal virulence on wheat by promoting spike colonization in the initial and middle stages of infection. In contrast, transgenic wheat plants with increased levels of pectin methyl esterification did not show any increase in resistance to the Δpme mutant, indicating that the infectivity of the fungus relies only to a certain degree on pectin degradation.

  8. Expression and activation of Daphnia pulex Caspase-3 are involved in regulation of aging.

    Science.gov (United States)

    Tong, Qiaoqiong; Zhang, Mengmeng; Cao, Xiao; Xu, Shanliang; Wang, Danli; Zhao, Yunlong

    2017-11-15

    Death-mediating proteases such as Caspases have been implicated in aging. Remarkably, active Caspase-3 can trigger widespread damage and degeneration, playing a key role in causing cell death. In order to explore the relationship between Caspase-3 and aging in Daphnia pulex, we cloned and analyzed the full-length cDNA sequence of its Caspase-3 gene. Both mRNA expression and activity of D. pulex Caspase-3 increased with age. Moreover, different forms of Caspase-3 appeared with aging. The expression of casp3-L was higher and decreased with age, while that of casp3-S was weak and increased with age, consistent with the trend in Caspase-3 activity. Mhc mRNA expression declined over time and was negatively correlated with age and Caspase-3. In situ hybridization results showed that Caspase-3 mRNA was expressed in different growth and reproduction stages, and its expression levels in embryos and larva were lower than that in adult D. pulex. Western blot analysis revealed the presence of Caspase-3 in the form of zymogens with a molecular weight of ~36kDa. Overall, this study explored age-associated gene regulation to provide a basis for the molecular mechanism of D. pulex reproductive conversion. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Relations of proliferative activities of gastric carcinoma cells to lymphatic involvement, venous invasion and prognosis

    Institute of Scientific and Technical Information of China (English)

    吴云飞; 徐惠绵; 陈峻青

    2004-01-01

    Background This study was to evaluate bivariate bromodeoxyuridine(BrdUrd)/DNA flow cytometric analysis in detection of gastric carcinoma and to study the relations of cellular BrdUrd labeling indices (LI), G2/M-phase fraction(G2/MPF) and DNA ploidy pattern to lymphatic involvement, venous invasion and prognosis.Methods Fresh tumor samples from 60 patients with gastric carcinoma were analyzed by bivariate BrdUrd/DNA flow cytometry. The results were correlated with lymphatic vessel invasion, lymphatic node metastasis, the number of matastatic lymphatic nodes, and venous invasion. Propidium iodide (PI) was used as a fluorescent probe for total cellular DNA, and a monoclonal antibody against BrdUrd was used as a probe for BrdUrd incorporated into DNA. Fluorescent-labeled goat anti-mouse antibody was used as a second antibody. S-phase fractions were measured by in vitro BrdUrd labeling, and DNA ploidy and G2/MPF were also measured. Comparison of survival was performed with the log-rank test, the Chi-square test for qualitative data, and Student's t test for quantu data. Results BrdUrd LI and G2/MPF values were significantly higher in tumors with lymphatic vessel invasion than in those without invasion respectively (P<0.01); the patients who had tumors with lymphatic vessel invasion showed a significantly poor prognosis (P<0.01). Both BrdUrd LI and G2/MPF values were significantly higher in tumors with lymphatic node metastasis than in those without metastasis (P<0.01). A statistical significant difference was noted in the 5-year survival rates between the patients with lymph node metastasis and those without metastasis. Compared with diploid carcinoma, the incidence of lymph node metastasis was significantly higher in aneuploid carcinoma (P<0.05), and the patients with aneuploid carcinoma showed a significantly poor prognosis (P<0.05). BrdUrd LI was significantly higher in patients with more than 5 metastatic lymph nodes than those with 1-4 metastatic lymph nodes (P<0

  10. Using sculpturing during speech therapy sessions in order to involve a child with ASD in joint activities

    Directory of Open Access Journals (Sweden)

    Vlasova L.I.

    2016-06-01

    Full Text Available Children with autism spectrum disorders often have particular hardships in participating in joint activities with an adult. This makes interventions more problematic and draws back the achievement of planned educational goals. At the early stages of speech therapy, the important goal of individual sessions with the majority of children with autism spectrum disorders is not speech development in itself but developing motivation for joint activities. To ensure successful interaction between an educator and a child, different techniques can be used, including those that are not typical for speech therapy sessions, for example, sculpturing as a preferred activity with enjoyable sensory input. The article describes tips for developing rapport with children with autistic disorders, and a strategy for involving them in joint activities and further development of their speech.

  11. Involvement of glutamate 97 in ion influx through photo-activated channelrhodopsin-2.

    Science.gov (United States)

    Tanimoto, Saki; Sugiyama, Yuka; Takahashi, Tetsuo; Ishizuka, Toru; Yawo, Hiromu

    2013-01-01

    The light absorption of a channelrhodopsin-2 (ChR2) is followed by conformational changes to the molecule, which allows the channel structure to become permeable to cations. Previously, a single point mutation in ChR2, which replaces glutamate residue 97 with a nonpolar alanine (E97A), was found to attenuate the photocurrent, suggesting that the E97 residue is involved in ion flux regulation. Here, the significance of E97 and its counterpart ChR1 (E136) were extensively studied by mutagenesis, whereby we replaced these glutamates with aspartate (D), glutamine (Q) or arginine (R). We found that the charge at this position strongly influences ion permeation and that the photocurrents were attenuated in the order of ChR2>E97D≈E97Q>E97R. We observed similar results with our chimeric/synthetic/artificial construct, ChR-wide receiver (ChRWR), which contains the first to fifth transmembrane helices of ChR1. The E-to-Q or E-to-R mutations, but not the E-to-D mutation, strongly retarded the sensitivity to the Gd(3+)-dependent blocking of the ChR1 or ChR2 channels. Our results suggest that the glutamate residue at this position lies in the outer pore, where it interacts with a cation to facilitate dehydration, and that this residue is the primary binding target of Gd(3+).

  12. The gastroprotective effect of menthol: involvement of anti-apoptotic, antioxidant and anti-inflammatory activities.

    Directory of Open Access Journals (Sweden)

    Ariane Leite Rozza

    Full Text Available The aim of this research was to investigate the anti-apoptotic, antioxidant and anti-inflammatory properties of menthol against ethanol-induced gastric ulcers in rats. Wistar rats were orally treated with vehicle, carbenoxolone (100 mg/kg or menthol (50 mg/kg and then treated with ethanol to induce gastric ulcers. After euthanasia, stomach samples were prepared for histological slides and biochemical analyses. Immunohistochemical analyses of the cytoprotective and anti-apoptotic heat-shock protein-70 (HSP-70 and the apoptotic Bax protein were performed. The neutrophils were manually counted. The activity of the myeloperoxidase (MPO was measured. To determine the level of antioxidant functions, the levels of glutathione (GSH, glutathione peroxidase (GSH-Px, glutathione reductase (GR and superoxide dismutase (SOD were measured using ELISA. The levels of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α and interleukin-6 (IL-6 and the anti-inflammatory cytokine interleukin-10 (IL-10 were assessed using ELISA kits. The menthol treated group presented 92% gastroprotection compared to the vehicle-treated group. An increased immunolabeled area was observed for HSP-70, and a decreased immunolabeled area was observed for the Bax protein in the menthol treated group. Menthol treatment induced a decrease in the activity of MPO and SOD, and the protein levels of GSH, GSH-Px and GR were increased. There was also a decrease in the levels of TNF-α and IL-6 and an increase in the level of IL-10. In conclusion, oral treatment with menthol displayed a gastroprotective activity through anti-apoptotic, antixidant and anti-inflammatory mechanisms.

  13. The gastroprotective effect of menthol: involvement of anti-apoptotic, antioxidant and anti-inflammatory activities.

    Science.gov (United States)

    Rozza, Ariane Leite; Meira de Faria, Felipe; Souza Brito, Alba Regina; Pellizzon, Cláudia Helena

    2014-01-01

    The aim of this research was to investigate the anti-apoptotic, antioxidant and anti-inflammatory properties of menthol against ethanol-induced gastric ulcers in rats. Wistar rats were orally treated with vehicle, carbenoxolone (100 mg/kg) or menthol (50 mg/kg) and then treated with ethanol to induce gastric ulcers. After euthanasia, stomach samples were prepared for histological slides and biochemical analyses. Immunohistochemical analyses of the cytoprotective and anti-apoptotic heat-shock protein-70 (HSP-70) and the apoptotic Bax protein were performed. The neutrophils were manually counted. The activity of the myeloperoxidase (MPO) was measured. To determine the level of antioxidant functions, the levels of glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and superoxide dismutase (SOD) were measured using ELISA. The levels of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and the anti-inflammatory cytokine interleukin-10 (IL-10) were assessed using ELISA kits. The menthol treated group presented 92% gastroprotection compared to the vehicle-treated group. An increased immunolabeled area was observed for HSP-70, and a decreased immunolabeled area was observed for the Bax protein in the menthol treated group. Menthol treatment induced a decrease in the activity of MPO and SOD, and the protein levels of GSH, GSH-Px and GR were increased. There was also a decrease in the levels of TNF-α and IL-6 and an increase in the level of IL-10. In conclusion, oral treatment with menthol displayed a gastroprotective activity through anti-apoptotic, antixidant and anti-inflammatory mechanisms.

  14. Spiroketal formation and modification in avermectin biosynthesis involves a dual activity of AveC.

    Science.gov (United States)

    Sun, Peng; Zhao, Qunfei; Yu, Futao; Zhang, Hua; Wu, Zhuhua; Wang, Yinyan; Wang, Yan; Zhang, Qinglin; Liu, Wen

    2013-01-30

    Avermectins (AVEs), which are widely used for the treatment of agricultural parasitic diseases, belong to a family of 6,6-spiroketal moiety-containing, macrolide natural products. AVE biosynthesis is known to employ a type I polyketide synthase (PKS) system to assemble the molecular skeleton for further functionalization. It remains unknown how and when spiroketal formation proceeds, particularly regarding the role of AveC, a unique protein in the pathway that shares no sequence homology to any enzyme of known function. Here, we report the unprecedented, dual function of AveC by correlating its activity with spiroketal formation and modification during the AVE biosynthetic process. The findings in this study were supported by characterizing extremely unstable intermediates, products and their spontaneous derivative products from the simplified chemical profile and by comparative analysis of in vitro biotransformations and in vivo complementations mediated by AveC and MeiC (the counterpart in biosynthesizing the naturally occurring, AVE-like meilingmycins). AveC catalyzes the stereospecific spiroketalization of a dihydroxy-ketone polyketide intermediate and the optional dehydration to determine the regiospecific saturation characteristics of spiroketal diversity. These reactions take place between the closures of the hexene ring and 16-membered macrolide and the formation of the hexahydrobenzofuran unit. MeiC can replace the spirocyclase activity of AveC, but it lacks the independent dehydratase activity. Elucidation of the generality and specificity of AveC-type proteins allows for the rationalization of previously published results that were not completely understood, suggesting that enzyme-mediated spiroketal formation was initially underestimated, but is, in fact, widespread in nature for the control of stereoselectivity.

  15. Regulation of ERK1/2 activity by ghrelin-activated growth hormone secretagogue receptor 1A involves a PLC/PKCɛ pathway

    Science.gov (United States)

    Mousseaux, Delphine; Le Gallic, Lionel; Ryan, Joanne; Oiry, Catherine; Gagne, Didier; Fehrentz, Jean-Alain; Galleyrand, Jean-Claude; Martinez, Jean

    2006-01-01

    The growth hormone secretagogue receptor 1a (GHSR-1a) is a G-protein coupled receptor, involved in the biological actions of ghrelin by triggering inositol phosphates and calcium intracellular second messengers. It has also been reported that ghrelin could activate the 44- and 42-kDa extracellular signal-regulated protein kinases (ERK1/2) in different cell lines, but it is not clear whether this regulation is GHSR-1a dependent or not. To provide direct evidence for the coupling of GHSR-1a to ERK1/2 activation, this pathway has been studied in a heterologous expression system. Thus, in Chinese hamster ovary (CHO) cells we showed that ghrelin induced, via the human GHSR-1a, a transient and dose-dep endent activation of ERK1/2 leading to activation of the transcriptional factor Elk1. We then investigated the precise mechanisms involved in GHSR-1a-mediated ERK1/2 activation using various specific inhibitors and dominant-negative mutants and found that internalization of GHSR-1a was not necessary. Our results also indicate that phospholipase C (PLC) was involved in GHSR-1a-mediated ERK1/2 activation, however, pathways like tyrosine kinases, including Src, and phosphoinositide 3-kinases were not found to be involved. GHSR-1a-mediated ERK1/2 activation was abolished both by a general protein kinase C (PKC) inhibitor, Gö6983, and by PKC depletion using overnight pretreatment with phorbol ester. Moreover, the calcium chelator, BAPTA-AM, and the inhibitor of conventional PKCs, Gö6976, had no effect on the GHSR-1a-mediated ERK1/2 activation, suggesting the involvement of novel PKC isoforms (ɛ, δ), but not conventional or atypical PKCs. Further analyses suggest that PKCɛ is required for the activation of ERK1/2. Taken together, these data suggest that ghrelin, through GHSR-1a, activates the Elk1 transcriptional factor and ERK1/2 by a PLC- and PKCɛ-dependent pathway. PMID:16582936

  16. Leadership, management and teamwork learning through an extra-curricular project for medical students: descriptive study

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    Maria Lucia da Silva Germano Jorge

    Full Text Available CONTEXT AND OBJECTIVE: Professionalism in medicine requires preparation for the globalized world. Our objective was to describe a project that introduces medical students to the community, hospital and laboratory activities, thereby allowing them to gain experience in people management, leadership and teamwork.DESIGN AND SETTING: Descriptive study of the process applied at a philanthropic medical school in Curitiba, Paraná.METHOD: Inclusion of management and leadership practices as part of the medical degree program.RESULTS: The study groups consisted of fifteen students. After six months, any of the participants could be elected as a subcoordinator, with responsibility for managing tasks and representing the team in hospital departments and the community. The activities required increasing levels of responsibility. In medical schools, students' involvement in practical activities is often limited to observation. They are not required to take responsibilities or to interact with other students and stakeholders. However, they will become accountable, which thus has an adverse effect on all involved. The learning space described here aims to fill this gap by bringing students closer to the daily lives and experiences of healthcare professionals.CONCLUSION: Being a physician requires not only management and leadership, but also transferrable competencies, communication and critical thinking. These attributes can be acquired through experience of teamwork, under qualified supervision from teaching staff. Students are thus expected to develop skills to deal with and resolve conflicts, learn to share leadership, prepare others to help and replace them, adopt an approach based on mutual responsibility and discuss their performance.

  17. Adolescent pathways to adulthood drinking: sport activity involvement is not necessarily risky or protective.

    Science.gov (United States)

    Peck, Stephen C; Vida, Mina; Eccles, Jacquelynne S

    2008-05-01

    Use pattern-centered methods to examine how adolescents' alcohol use and sports activities are related both to childhood sport and problem behavior and to heavy drinking in early adulthood. The data used in this study come from four waves of the Michigan Study of Adolescent Life Transitions (MSALT) that began in 1983, when participants were approximately age 12, and continued into early adulthood, when participants were approximately age 28. Sixty per cent of the approximately 1000 MSALT youth living in south-eastern Michigan were females and 97% were European American. Approximately 28% of one or both parents held at least a college degree, and 45% held a high school diploma or lower. Pattern-centered analyses revealed that the relation between adolescent sport activity and age 28 heavy alcohol use obtained primarily for sport participants who were also using more than the average amount of alcohol and other drugs at age 18. Similarly, children who were characterized by relatively high levels of sport participation, aggression and other problem behavior at age 12 were more likely than expected by chance to become sport participants who used more than the average amount of alcohol and other drugs at age 18. The results indicate that childhood problem behavior and adolescent sport participation can, but do not necessarily, presage heavy drinking in adulthood and that pattern-centered analytical techniques are useful for revealing such theoretically generated predictions.

  18. Acclimation of microalgae to wastewater environments involves increased oxidative stress tolerance activity.

    Science.gov (United States)

    Osundeko, Olumayowa; Dean, Andrew P; Davies, Helena; Pittman, Jon K

    2014-10-01

    A wastewater environment can be particularly toxic to eukaryotic microalgae. Microalgae can adapt to these conditions but the specific mechanisms that allow strains to tolerate wastewater environments are unclear. Furthermore, it is unknown whether the ability to acclimate microalgae to tolerate wastewater is an innate or species-specific characteristic. Six different species of microalgae (Chlamydomonas debaryana, Chlorella luteoviridis, Chlorella vulgaris, Desmodesmus intermedius, Hindakia tetrachotoma and Parachlorella kessleri) that had never previously been exposed to wastewater conditions were acclimated over an 8-week period in secondary-treated municipal wastewater. With the exception of C. debaryana, acclimation to wastewater resulted in significantly higher growth rate and biomass productivity. With the exception of C. vulgaris, total chlorophyll content was significantly increased in all acclimated strains, while all acclimated strains showed significantly increased photosynthetic activity. The ability of strains to acclimate was species-specific, with two species, C. luteoviridis and P. kessleri, able to acclimate more efficiently to the stress than C. debaryana and D. intermedius. Metabolic fingerprinting of the acclimated and non-acclimated microalgae using Fourier transform infrared spectroscopy was able to differentiate strains on the basis of metabolic responses to the stress. In particular, strains exhibiting greater stress response and altered accumulation of lipids and carbohydrates could be distinguished. The acclimation to wastewater tolerance was correlated with higher accumulation of carotenoid pigments and increased ascorbate peroxidase activity.

  19. Omega-3 Polyunsaturated Fatty Acids Attenuate Fibroblast Activation and Kidney Fibrosis Involving MTORC2 Signaling Suppression

    Science.gov (United States)

    Zeng, Zhifeng; Yang, Haiyuan; Wang, Ying; Ren, Jiafa; Dai, Yifan; Dai, Chunsun

    2017-01-01

    Epidemiologic studies showed the correlation between the deficiency of omega-3 polyunsaturated fatty acids (n-3 PUFAs) and the progression of chronic kidney diseases (CKD), however, the role and mechanisms for n-3 PUFAs in protecting against kidney fibrosis remain obscure. In this study, NRK-49F cells, a rat kidney interstitial fibroblast cell line, were stimulated with TGFβ1. A Caenorhabditis elegans fat-1 transgenic mouse model in which n-3 PUFAs are endogenously produced from n-6 PUFAs owing to the expression of n-3 fatty acid desaturase were deployed. Docosahexaenoic acid (DHA), one member of n-3 PUFAs family, could suppress TGFβ1-induced fibroblast activation at a dose and time dependent manner. Additionally, DHA could largely inhibit TGFβ1-stimulated Akt but not S6 or Smad3 phosphorylation at a time dependent manner. To decipher the role for n-3 PUFAs in protecting against kidney fibrosis, fat-1 transgenic mice were operated with unilateral ureter obstruction (UUO). Compared to the wild types, fat-1 transgenics developed much less kidney fibrosis and inflammatory cell accumulation accompanied by less p-Akt (Ser473), p-Akt (Thr308), p-S6 and p-Smad3 in kidney tissues at day 7 after UUO. Thus, n-3 PUFAs can attenuate fibroblast activation and kidney fibrosis, which may be associated with the inhibition of mTORC2 signaling. PMID:28393852

  20. Effects of three types of physical activity on reduction of metabolic parameters involved in cardiovascular risk

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    Petrović-Oggiano Gordana

    2009-01-01

    Full Text Available The aim of present study was to investigate the effects of three different types of physical activity on reduction of the metabolic parameters mainly responsible for cardiovascular diseases. This prospective-intervention study was performed at the 'ČIGOTA' Thyroid Institute on Mt. Zlatibor (Serbia between August 2004 and June 2006. Sixty-eight overweight/obese patients aged 40-70 years with hyperlipidemia were divided into three groups according to their weight and overall health. The program of physical workout included: group I - fast walking; group II - gymnastic exercises and specially chosen exercises in the swimming pool; and group III - combined physical training of higher intensity and greater length. All patients were also on a special reduced diet of 1000 kcal per day, the AHA step-2 diet. We monitored the body mass index, body composition, glucose, cholesterol (total, LDL-, and HDL-, and triglycerides before, during, and after the intervention. After 2 and particularly 12 weeks of intervention, a significant improvement of all metabolic parameters was achieved in all three groups of patients. Although most patients completed the study with normal values of all parameters, the most desirable results were achieved in group III (combined exercises with an average energy expenditure of 900 kcal per day. Our research indicates that a specially conceived program of physical activity and diet intervention resulted in significant reduction of cardiovascular risk factors.

  1. GM1 ganglioside is involved in epigenetic activation loci of neuronal cells

    Science.gov (United States)

    Tsai, Yi-Tzang; Itokazu, Yutaka; Yu, Robert K.

    2015-01-01

    Gangliosides are sialic acid-containing glycosphingolipids that are most abundant in the nerve tissues. The quantity and expression pattern of gangliosides in brain change drastically throughout development and are mainly regulated through stage-specific expression of glycosyltransferase (ganglioside synthase) genes. We previously demonstrated that acetylation of histones H3 and H4 on the N-acetylgalactosaminyltransferase I (GalNAcT, GA2/GM2/GD2/GT2-synthase) gene promoter resulted in recruitment of trans-activation factors. In addition, we reported that epigenetic activation of the GalNAcT gene was also detected as accompanied by an apparent induction of neuronal differentiation in neural stem cells responding to an exogenous supplement of ganglioside GM1. Here, we present evidence supporting the concept that nuclear GM1 is associated with gene regulation in neuronal cells. We found that nuclear GM1 binds acetylated histones on the promoters of the GalNAcT and NeuroD1 genes in differentiated neurons. Our study demonstrates for the first time that GM1 interacts with chromatin via acetylated histones at the nuclear periphery of neuronal cells. PMID:26498762

  2. Griflola frondosa (GF) produces significant antidepressant effects involving AMPA receptor activation in mice.

    Science.gov (United States)

    Bao, Hongkun; Ran, Pengzhan; Sun, Lijuan; Hu, Weihong; Li, Hongliang; Xiao, Chunjie; Zhu, Keming; Du, Jing

    2017-12-01

    Griflola frondosa (Fr) S.F. Gray (Meripilaceae) (GF) is a medical mushroom, and its regulation of the immune system is of interest for the treatment of mood disorders. α-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors are the central mediator for the treatment of depression. This study examines the antidepressant effects of GF and the role of AMPA in these antidepressant effects. The CD-1 mice were fed with GF- or Pleurotus ostreatus [(Jacq.: Fr) Kumm (Pleurotaceae)] (PO)-containing food for 1 day or 5 days. The antidepressant effects was determined in the tail suspension test (TST), forced swim test (FST), and open field test (OFT). The involvement of AMPA receptors was determined by the application of the AMPA-specific blocker GYKI 52466. Treatments with 20%, 33% or 50% of GF-containing food significantly decreased the immobility time (63.6, 56.9, and 52.0% in TST; and 50.8, 43.2, and 38.2% in FST) after 1 day and (62.3, 51.8, and 52.8% in TST; and 49.5, 45.1, and 40.3% in FST) after 5 days. GF-containing food did not cause hyperactive effects in the OFT. The antidepressant effects of the 33% of GF-containing food (down-to 51.3% in 1-day TST and 46.8% in 5-day FST) were significantly stronger than that of the 33% of PO-containing food (down-to 85.5% in 1-day TST and 82.0% in 5-day FST). AMPA-specific blocker GYKI 52466 was able to block the antidepressant effects of the GF-containing food. GF demonstrated the potential as a safe medical food supplement for the patient with depression.

  3. Activation of Akt by advanced glycation end products (AGEs: involvement of IGF-1 receptor and caveolin-1.

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    Su-Jung Yang

    Full Text Available Diabetes is characterized by chronic hyperglycemia, which in turn facilitates the formation of advanced glycation end products (AGEs. AGEs activate signaling proteins such as Src, Akt and ERK1/2. However, the mechanisms by which AGEs activate these kinases remain unclear. We examined the effect of AGEs on Akt activation in 3T3-L1 preadipocytes. Addition of AGEs to 3T3-L1 cells activated Akt in a dose- and time-dependent manner. The AGEs-stimulated Akt activation was blocked by a PI3-kinase inhibitor LY 294002, Src inhibitor PP2, an antioxidant NAC, superoxide scavenger Tiron, or nicotinamide adenine dinucleotide phosphate (NAD(PH oxidase inhibitor DPI, suggesting the involvement of Src and NAD(PH oxidase in the activation of PI3-kinase-Akt pathway by AGEs. AGEs-stimulated Src tyrosine phosphorylation was inhibited by NAC, suggesting that Src is downstream of NAD(PH oxidase. The AGEs-stimulated Akt activity was sensitive to Insulin-like growth factor 1 receptor (IGF-1R kinase inhibitor AG1024. Furthermore, AGEs induced phosphorylation of IGF-1 receptorβsubunit (IGF-1Rβ on Tyr1135/1136, which was sensitive to PP2, indicating that AGEs stimulate Akt activity by transactivating IGF-1 receptor. In addition, the AGEs-stimulated Akt activation was attenuated by β-methylcyclodextrin that abolishes the structure of caveolae, and by lowering caveolin-1 (Cav-1 levels with siRNAs. Furthermore, addition of AGEs enhanced the interaction of phospho-Cav-1 with IGF-1Rβ and transfection of 3T3-L1 cells with Cav-1 Y14F mutants inhibited the activation of Akt by AGEs. These results suggest that AGEs activate NAD(PH oxidase and Src which in turn phosphorylates IGF-1 receptor and Cav-1 leading to activation of IGF-1 receptor and the downstream Akt in 3T3-L1 cells. AGEs treatment promoted the differentiation of 3T3-L1 preadipocytes and addition of AG1024, LY 294002 or Akt inhibitor attenuated the promoting effect of AGEs on adipogenesis, suggesting that IGF-1

  4. Severity of nicotine dependence modulates cue-induced brain activity in regions involved in motor preparation and imagery.

    Science.gov (United States)

    Smolka, Michael N; Bühler, Mira; Klein, Sabine; Zimmermann, Ulrich; Mann, Karl; Heinz, Andreas; Braus, Dieter F

    2006-03-01

    In nicotine-dependent subjects, cues related to smoking elicit activity in brain regions linked to attention, memory, emotion and motivation. Cue-induced brain activation is associated with self-reported craving but further correlates are widely unknown. This study was conducted to investigate whether brain activity elicited by smoking cues increases with severity of nicotine dependence and intensity of cue-elicited craving. Ten healthy male smokers whose degree of nicotine dependence ranged from absent to severe were investigated. Visual smoking cues and neutral stimuli were presented in a block design during functional magnetic resonance imaging (fMRI). Using multiple linear regression analysis, the blood oxygen level dependent (BOLD) response to smoking cues was correlated with severity of nicotine dependence assessed with the Fagerström Test of Nicotine Dependence (FTND) and with cue-induced craving. Significant positive correlations between the BOLD activity and FTND scores were found in brain areas related to visuospatial attention (anterior cingulate cortex, parietal cortex, parahippocampal gyrus and cuneus) and in regions involved in motor preparation and imagery (primary and premotor cortex, supplementary motor area). Intensity of cue-induced craving was significantly associated with greater BOLD activation in mesocorticolimbic areas engaged in incentive motivation and in brain regions related to episodic memory. Our study suggests that severity of nicotine dependence and intensity of craving are independently associated with cue-induced brain activation in separate neuronal networks. The observed association between severity of dependence and brain activity in regions involved in allocation of attention, motor preparation and imagery might reflect preparation of automated drug taking behavior thereby facilitating cue-induced relapse.

  5. Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells

    Science.gov (United States)

    Hire, Rupali R.; Srivastava, Shalini; Davis, Melissa B.; Kumar Konreddy, Ananda; Panda, Dulal

    2017-01-01

    Crocin, a component of saffron spice, is known to have an anticancer activity. However, the targets of crocin are not known. In this study, crocin was found to inhibit the proliferation of HCC70, HCC1806, HeLa and CCD1059sk cells by targeting microtubules. Crocin depolymerized both the interphase and mitotic microtubules of different cancer cells, inhibited mitosis and induced multipolar spindle formation in these cells. In vitro, crocin inhibited the assembly of pure tubulin as well as the assembly of microtubule-associated protein rich tubulin. Electron microscopic analysis showed that crocin inhibited microtubule assembly while it induced aggregation of tubulin at higher concentrations. Crocin co-eluted with tubulin suggesting that it binds to tubulin. Vinblastine inhibited the binding of crocin to tubulin while podophyllotoxin did not inhibit the crocin binding indicating that crocin binds at the vinblastine site on tubulin. The results suggested that crocin inhibited cell proliferation mainly by disrupting the microtubule network. PMID:28337976

  6. Neuroprotective activity of stiripentol with a possible involvement of voltage-dependent calcium and sodium channels.

    Science.gov (United States)

    Verleye, Marc; Buttigieg, Dorothée; Steinschneider, Rémy

    2016-02-01

    A growing body of data has shown that recurrent epileptic seizures may be caused by an excessive release of the excitatory neurotransmitter glutamate in the brain. Glutamatergic overstimulation results in massive neuronal influxes of calcium and sodium through N-methyl-D-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, and kainic acid glutamate subtype receptors and also through voltage-gated calcium and sodium channels. These persistent and abnormal sodium and calcium entry points have deleterious consequences (neurotoxicity) for neuronal function. The therapeutic value of an antiepileptic drug would include not only control of seizure activity but also protection of neuronal tissue. The present study examines the in vitro neuroprotective effects of stiripentol, an antiepileptic compound with γ-aminobutyric acidergic properties, on neuronal-astroglial cultures from rat cerebral cortex exposed to oxygen-glucose deprivation (OGD) or to glutamate (40 µM for 20 min), two in vitro models of brain injury. In addition, the affinity of stiripentol for the different glutamate receptor subtypes and the interaction with the cell influx of Na(+) and of Ca(2+) enhanced by veratridine and NMDA, respectively, are assessed. Stiripentol (10-100 µM) included in the culture medium during OGD or with glutamate significantly increased the number of surviving neurons relative to controls. Stiripentol displayed no binding affinity for different subtypes of glutamate receptors (IC50  >100 µM) but significantly blocked the entry of Na(+) and Ca(2+) activated by veratridine and NMDA, respectively. These results suggest that Na(+) and Ca(2+) channels could contribute to the neuroprotective properties of sitiripentol.

  7. Administrator Interest is Perceived to Encourage Faculty and Librarian Involvement in Open Access Activities

    Directory of Open Access Journals (Sweden)

    Eamon C. Tewell

    2014-09-01

    Full Text Available A Review of: Reinsfelder, T.L., & Anderson, J.A. (2013. Observations and perceptions of academic administrator influence on open access initiatives. Journal of Academic Librarianship, 39(6: 481-487. http://dx.doi.org/10.1016/j.acalib.2013.08.014 Abstract Objective – To better understand the roles and influence of senior-level academic administrators, such as provosts, on open access (OA activities at the institutional level, including whether librarians perform these activities regardless of administrative interest. Design – Web-based survey questionnaire combined with multiple regression analysis. Settings – The research was conducted online using surveys emailed to potential participants at not-for-profit public and private academic institutions in the United States with a FTE of greater than 1000. Subjects – Academic library directors at selected colleges and universities. Methods – Using directory information from the National Center for Education Statistics (NCES and filtering institutions according to not-for-profit status, size, and special focus, a survey sample of 1135 colleges and universities was obtained. Library websites were used to acquire contact information for library directors. In summer 2012 the 43-item survey questionnaire was distributed to respondents online using Qualtrics. The four primary variables were each comprised of multiple questionnaire items and validated using factor analysis, and the data was explored using multiple regression. Main Results – The survey received 298 respondents for a 26% response rate, though the number of incomplete responses is not stated. Among four stakeholder groups (faculty, publishers, librarians, and senior academic administrators, library directors perceived librarians as having the greatest influence in regards to the adoption of open access (mean = .7056, followed by faculty (.3792, administrators (.1881, and publishers as having a negative impact (–.3684. A positive

  8. Peroxidase activity and involvement in the oxidative stress response of roseobacter denitrificans truncated hemoglobin.

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    Yaya Wang

    Full Text Available Roseobacter denitrificans is a member of the widespread marine Roseobacter genus. We report the first characterization of a truncated hemoglobin from R. denitrificans (Rd. trHb that was purified in the heme-bound form from heterologous expression of the protein in Escherichia coli. Rd. trHb exhibits predominantly alpha-helical secondary structure and absorbs light at 412, 538 and 572 nm. The phylogenetic classification suggests that Rd. trHb falls into group II trHbs, whereas sequence alignments indicate that it shares certain important heme pocket residues with group I trHbs in addition to those of group II trHbs. The resonance Raman spectra indicate that the isolated Rd. trHb contains a ferric heme that is mostly 6-coordinate low-spin and that the heme of the ferrous form displays a mixture of 5- and 6-coordinate states. Two Fe-His stretching modes were detected, notably one at 248 cm-1, which has been reported in peroxidases and some flavohemoglobins that contain an Fe-His-Asp (or Glu catalytic triad, but was never reported before in a trHb. We show that Rd. trHb exhibits a significant peroxidase activity with a (kcat/Km value three orders of magnitude higher than that of bovine Hb and only one order lower than that of horseradish peroxidase. This enzymatic activity is pH-dependent with a pKa value ~6.8. Homology modeling suggests that residues known to be important for interactions with heme-bound ligands in group II trHbs from Mycobacterium tuberculosis and Bacillus subtilis are pointing toward to heme in Rd. trHb. Genomic organization and gene expression profiles imply possible functions for detoxification of reactive oxygen and nitrogen species in vivo. Altogether, Rd. trHb exhibits some distinctive features and appears equipped to help the bacterium to cope with reactive oxygen/nitrogen species and/or to operate redox biochemistry.

  9. Calmodulin Involvement in Stress-Activated Nuclear Localization of Albumin in JB6 Epithelial Cells.

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    Weber, Thomas J.; Negash, Sewite; Smallwood, Heather S.; Ramos, Kenneth S.; Thrall, Brian D.; Squier, Thomas C.

    2004-06-15

    We report that in response to oxidative stress, albumin is translocated to the nucleus where it binds in concert with known transcription factors to an antioxidant response element (ARE), which controls the expression of glutathione-S-transferase and other antioxidant enzymes, functioning to mediate adaptive cellular responses. To investigate the mechanisms underlying this adaptive cell response, we have identified linkages between calcium signaling and the nuclear translocation of albumin in JB6 epithelial cells. Under resting conditions, albumin and the calcium regulatory protein, calmodulin (CaM), co-immunoprecipitate using antibodies against either protein, indicating a tight association. Calcium activation of CaM disrupts the association between CaM and albumin, suggesting that transient increases in cytosolic calcium levels function to mobilize intracellular albumin to facilitate its translocation into the nucleus. Likewise, nuclear translocation of albumin is induced by exposure of cells to hydrogen peroxide or a phorbol ester, indicating a functional linkage between reactive oxygen species, calcium, and PKC-signaling pathways. Inclusion of an antioxidant enzyme (i.e., superoxide dismutase) blocks nuclear translocation, suggesting that the oxidation of sensitive proteins functions to coordinate the adaptive cellular response. These results suggest that elevated calcium transients, and associated increases in reactive oxygen species, contribute to adaptive cellular responses through the mobilization and nuclear translocation of cellular albumin to mediate the transcriptional regulation of antioxidant responsive elements.

  10. Microwave synthesis, spectral, thermal, and antimicrobial activities of some transition metal complexes involving 5-bromosalicylaldehyde moiety

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    Rajendra K. Jain

    2012-07-01

    Full Text Available The coordination complexes of Co(II, Ni(II and Cu(II derived from 5-bromosalicylidene-3,4-dimethylaniline (BSMA and 5-bromosalicylidene-3,4-dichloroaniline (BSCA have been synthesized by conventional as well as microwave methods. These compounds have been characterized by elemental analysis, FT-IR, molar conductance, electronic spectra, 1H-NMR, FAB-mass, ESR, magnetic susceptibility, electrical conductivity and thermal analysis. The complexes are coloured and stable in air. Analytical data revealed that all the complexes exhibited 1:2 (metal: ligand ratio with coordination number 4 or 6. IR data shows that the ligand coordinates with the metal ions in a bidentate manner through the phenolic oxygen and azomethine nitrogen. FAB-mass and thermal data show degradation pattern of the complexes. Solid state electrical conductivity studies reflect semiconducting nature of the complexes. The Schiff base and metal complexes show a good activity against the Gram-positive bacteria; Staphylococcus aureus and Gram-negative bacteria; Escherichia coli and fungi Aspergillus niger and Candida albicans.

  11. Betacoronavirus Adaptation to Humans Involved Progressive Loss of Hemagglutinin-Esterase Lectin Activity.

    Science.gov (United States)

    Bakkers, Mark J G; Lang, Yifei; Feitsma, Louris J; Hulswit, Ruben J G; de Poot, Stefanie A H; van Vliet, Arno L W; Margine, Irina; de Groot-Mijnes, Jolanda D F; van Kuppeveld, Frank J M; Langereis, Martijn A; Huizinga, Eric G; de Groot, Raoul J

    2017-03-08

    Human beta1-coronavirus (β1CoV) OC43 emerged relatively recently through a single zoonotic introduction. Like related animal β1CoVs, OC43 uses 9-O-acetylated sialic acid as receptor determinant. β1CoV receptor binding is typically controlled by attachment/fusion spike protein S and receptor-binding/receptor-destroying hemagglutinin-esterase protein HE. We show that following OC43's introduction into humans, HE-mediated receptor binding was selected against and ultimately lost through progressive accumulation of mutations in the HE lectin domain. Consequently, virion-associated receptor-destroying activity toward multivalent glycoconjugates was reduced and altered such that some clustered receptor populations are no longer cleaved. Loss of HE lectin function was also observed for another respiratory human coronavirus, HKU1. This thus appears to be an adaptation to the sialoglycome of the human respiratory tract and for replication in human airways. The findings suggest that the dynamics of virion-glycan interactions contribute to host tropism. Our observations are relevant also to other human respiratory viruses of zoonotic origin, particularly influenza A virus. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. IL-33 accelerates cutaneous wound healing involved in upregulation of alternatively activated macrophages.

    Science.gov (United States)

    Yin, Hui; Li, Xiangyong; Hu, Shilian; Liu, Tao; Yuan, Baohong; Gu, Hongbiao; Ni, Qian; Zhang, Xiaofan; Zheng, Fang

    2013-12-01

    IL-33 is a recently recognized member of the IL-1 family and has been best identified as a potent inducer of Th2-type immune responses. Increasing evidence, however, indicates that IL-33 also represents an important mediator of mucosal healing and epithelial restoration and repair. In this study, we further explore the potential effect of IL-33 in cutaneous wound healing. A full-thickness skin wound was generated on the back of mice and treated with IL-33 or vehicle intraperitoneally. Our results revealed that the levels of IL-33 mRNA and protein were significantly enhanced in incisional wound skin. Meantime, administration of IL-33 obviously accelerated wound healing with wounds gaping narrower and exhibiting enhanced reepithelialization. IL-33 upregulation also promoted the collagen deposition and the expression of extracellular matrix (ECM)-associated genes such as fibronectin and collagen IIIa, which implies a direct effect of IL-33 on matrix synthesis. Furthermore, IL-33 facilitated the development of alternatively activated macrophages (AAM) in incisional wound tissue, which closely related to resolution of inflammation and promotion of wound repair. Taken together, these findings suggest that IL-33 may play a pivotal role in maintenance of cutaneous homeostasis and acceleration of normal wound healing.

  13. Environmental parameters altered by climate change affect the activity of soil microorganisms involved in bioremediation.

    Science.gov (United States)

    Alkorta, Itziar; Epelde, Lur; Garbisu, Carlos

    2017-09-15

    Bioremediation, based on the use of microorganisms to break down pollutants, can be very effective at reducing soil pollution. But the climate change we are now experiencing is bound to have an impact on bioremediation performance, since the activity and degrading abilities of soil microorganisms are dependent on a series of environmental parameters which are themselves being altered by climate change, such as soil temperature, moisture, amount of root exudates, etc. Many climate-induced effects on soil microorganisms occur indirectly through changes in plant growth and physiology derived from increased atmospheric CO2 concentrations and temperatures, the alteration of precipitation patterns, etc., with a concomitant effect on rhizoremediation performance (i.e. the plant-assisted microbial degradation of pollutants in the rhizosphere). But these effects are extremely complex and mediated by processes such as acclimation and adaptation. Besides, soil microorganisms form complex networks of interactions with a myriad of organisms from many taxonomic groups which will also be affected by climate change, further complicating data interpretation. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  14. The mix of laws involved in the activity of the companies in the aerospace field

    Directory of Open Access Journals (Sweden)

    Aurelian Virgil BALUTA

    2017-03-01

    Full Text Available The life of companies, including the aerospace companies, depends on the business cycle. The paper presents the trends of law in ascending and descending period of the business cycle. A point of the paper is the separation of military and civil law in aerospace, public and private law, national and corporate security systems. Also the laws to be apply in relation with public authorities, private organizations, citizens are approched. In the paper are included some keys for interpretation such as the hierarchy of social values. In modern times, the humans life, rights and property must be the main protected values. The paper shows the methods to be accepted for the analyse/analysis of law in aerospace field: logical analysis, hystorical method, comparative method, social research, experimental method. In the aerospace field each of them has some particularities. The classification of laws depending of economic impact in the aerospace field is an other section. There are presented implications on cost, income, receipts, payments, duration of the activities, other restrictions.

  15. Anti-Candida activity of geraniol involves disruption of cell membrane integrity and function.

    Science.gov (United States)

    Sharma, Y; Khan, L A; Manzoor, N

    2016-09-01

    Candidiasis is a major problem in immunocompromised patients. Candida, an opportunistic fungal pathogen, is a major health concern today as conventional drugs are highly toxic with undesirable side effects. Their fungistatic nature is responsible for drug resistance in continuously evolving strains. Geraniol, an acyclic monoterpene alcohol, is a component of several plant essential oils. In the present study, an attempt has been made to understand the antifungal activity of geraniol at the cell membrane level in three Candida species. With an MIC of 30-130μg/mL, this natural compound was fungicidal at concentrations 2×MIC. There was complete suppression of fungal growth at MIC values (growth curves) and encouragingly geraniol is non-toxic even at the concentrations approaching 5×MIC (hemolysis assay). Exposed cells showed altered morphology, wherein the cells appeared either broken or shrivelled up (SEM studies). Significant reduction was seen in ergosterol levels at sub-MIC and glucose-induced H(+) efflux at concentrations>MIC values. Our results suggest that geraniol disrupts cell membrane integrity by interfering with ergosterol biosynthesis and inhibiting the very crucial PM-ATPase. It may hence be used in the management and treatment of both superficial and invasive candidiasis but further studies are required to elaborate its mode of action.

  16. Collaborative modelling for active involvement of stakeholders in urban flood risk management

    Directory of Open Access Journals (Sweden)

    M. Evers

    2012-09-01

    Full Text Available This paper presents an approach to enhance the role of local stakeholders in dealing with urban floods. The concept is based on the DIANE-CM project (Decentralised Integrated Analysis and Enhancement of Awareness through Collaborative Modelling and Management of Flood Risk of the 2nd ERANET CRUE funding initiative. The main objective of the project was to develop and test an advanced methodology for enhancing the resilience of local communities to flooding. Through collaborative modelling, a social learning process was initiated that enhances the social capacity of the stakeholders due to the interaction process. The other aim of the project was to better understand how data from hazard and vulnerability analyses and improved maps, as well as from the near real-time flood prediction, can be used to initiate a public dialogue (i.e. collaborative mapping and planning activities in order to carry out more informed and shared decision-making processes and to enhance flood risk awareness. The concept of collaborative modelling was applied in two case studies: (1 the Cranbrook catchment in the UK, with focus on pluvial flooding; and (2 the Alster catchment in Germany, with focus on fluvial flooding. As a result of the interactive and social learning process, supported by sociotechnical instruments, an understanding of flood risk was developed amongst the stakeholders and alternatives for flood risk management for the respective case study area were jointly developed and ranked as a basis for further planning and management.

  17. Immunological identification of candidate proteins involved in regulating active shape changes of outer hair cells.

    Science.gov (United States)

    Knipper, M; Zimmermann, U; Köpschall, I; Rohbock, K; Jüngling, S; Zenner, H P

    1995-06-01

    By employing immunological methods, it has been demonstrated that myosin, myosin light chain (MLC) and myosin light chain kinase (MLCK) proteins in outer hair cells (OHC) are immunologically different from isoforms in platelets, smooth muscle and heart muscle, and are probably more related to isoforms found in red blood cells (RBC). Moreover, proteins related to band 3 protein (b3p) and protein 4.1 (p 4.1), ankyrin as well as fodrin and spectrin, but not glycophorin, have been identified in isolated OHCs. Both OHCs and RBC differ from other motile non-muscle cells in their lack of smooth muscle isoforms of actin, their common high levels of spectrin-, ankyrin- and band 3-like proteins, as well as the expression of the 80 kDa protein 4.1 isoform. The data support the notion that motility of OHC may be based upon regulation of the b3p/p 4.1/ankyrin complex, and thus may be reminiscent to the active shape changes in RBC.

  18. Hydralazine-induced vasodilation involves opening of high conductance Ca2+-activated K+ channels

    DEFF Research Database (Denmark)

    Bang, Lone; Nielsen-Kudsk, J E; Gruhn, N

    1998-01-01

    The purpose of this study was to investigate whether high conductance Ca2+-activated K+ channels (BK(Ca)) are mediating the vasodilator action of hydralazine. In isolated porcine coronary arteries, hydralazine (1-300 microM), like the K+ channel opener levcromakalim, preferentially relaxed...... contractions induced by K+ (20 mM) compared with K+ (80 mM). In addition, concentration-relaxation curves for hydralazine (pD2 = 5.38 +/- 0.06; Emax = 85.9 +/- 3.6%) were shifted 10-fold to the right by the BK(Ca) blockers tetraethylammonium (1 mM) and iberiotoxin (0.1 microM). In contrast, nimodipine (a Ca2......+-entry blocker), relaxed contractions induced by K+ (20 mM) and K+ (80 mM) equally and nimodipine-induced relaxations were neither antagonized by tetraethylammonium nor by iberiotoxin. In isolated perfused rat hearts, hydralazine (1 microM) increased coronary flow by 28.8 +/- 2.7%. Iberiotoxin (0.1 micro...

  19. Involvement of activation-induced cytidine deaminase in skin cancer development.

    Science.gov (United States)

    Nonaka, Taichiro; Toda, Yoshinobu; Hiai, Hiroshi; Uemura, Munehiro; Nakamura, Motonobu; Yamamoto, Norio; Asato, Ryo; Hattori, Yukari; Bessho, Kazuhisa; Minato, Nagahiro; Kinoshita, Kazuo

    2016-04-01

    Most skin cancers develop as the result of UV light-induced DNA damage; however, a substantial number of cases appear to occur independently of UV damage. A causal link between UV-independent skin cancers and chronic inflammation has been suspected, although the precise mechanism underlying this association is unclear. Here, we have proposed that activation-induced cytidine deaminase (AID, encoded by AICDA) links chronic inflammation and skin cancer. We demonstrated that Tg mice expressing AID in the skin spontaneously developed skin squamous cell carcinoma with Hras and Trp53 mutations. Furthermore, genetic deletion of Aicda reduced tumor incidence in a murine model of chemical-induced skin carcinogenesis. AID was expressed in human primary keratinocytes in an inflammatory stimulus-dependent manner and was detectable in human skin cancers. Together, the results of this study indicate that inflammation-induced AID expression promotes skin cancer development independently of UV damage and suggest AID as a potential target for skin cancer therapeutics.

  20. Phytochemicals from Ruta graveolens Activate TAS2R Bitter Taste Receptors and TRP Channels Involved in Gustation and Nociception

    Directory of Open Access Journals (Sweden)

    Giuseppe Mancuso

    2015-10-01

    Full Text Available Ruta graveolens (rue is a spontaneous plant in the Mediterranean area with a strong aroma and a very intense bitter taste, used in gastronomy and in folk medicine. From the leaves, stems and fruits of rue, we isolated rutin, rutamarin, three furanocoumarins, two quinolinic alkaloids, a dicoumarin and two long chain ketones. Bitter taste and chemesthetic properties have been evaluated by in vitro assays with twenty receptors of the TAS2R family and four TRP ion channels involved in gustation and nociception. Among the alkaloids, skimmianine was active as a specific agonist of T2R14, whereas kokusaginin did not activate any of the tested receptors. The furanocoumarins activates TAS2R10, 14, and 49 with different degrees of selectivity, as well as the TRPA1 somatosensory ion channel. Rutamarin is an agonist of TRPM5 and TRPV1 and a strong antagonist of TRPM8 ion channels.