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Sample records for extracted human molars

  1. CANINE ECTOPIC TREATMENT WITH FIRST MOLAR EXTRACTION

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    Angelica Margo

    2015-06-01

    Full Text Available Decision to extract or not and the type of tooth must be analyzed carefully in orthodontic treatment. Preferable tooth to be extracted was the tooth with large caries or restoration. Usually the type of tooth to be extracted was second molar (if the third molar appears, incisor, first molar, and combination of several teeth. Orthodontic treatment with molar extraction is more difficult to treat and the result is usually compromise. There are several considerations in extracting first molar such as tooth with large caries or restoration, hypoplasia, periapical disease, large discrepancy, high maxilla-mandibular plane angle, and cases with anterior open bite. Nowadays, orthodontic cases with molar extraction do not prolong the treatment time compared to premolar extraction case, but the anchorage system must be considered carefully. The present case was treated with extraction of first molar to solve anterior crowding with maximum anchorage at the upper jaw and using Nance Holding Appliance.

  2. Does fixed retention prevent overeruption of unopposed mandibular second molars in maxillary first molar extraction cases?

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    Christos Livas

    2016-01-01

    Full Text Available Abstract Background The objective of this study was to investigate whether multistranded fixed retainers prevented overeruption of unopposed mandibular second molars in maxillary first molar extraction cases. Methods The panoramic radiographs of 65 Class II Division 1 Caucasian Whites (28 females, 37 males consecutively treated with bilateral maxillary first molar extraction and the Begg technique, and with records taken after treatment (T1 and in retention (T2, were withdrawn from private practice records. After appliance removal, mandibular second molars were retained with sectional wires till at least T2 in case of lack of occlusal contact with the antagonist. The subjects were assigned to study-retention and control-nonretention groups based on the retention status of mandibular second molars. Radiographic analysis was carried out to determine inclination of mandibular molars and the resulting movement of second molar centroids. Parametric and nonparametric tests were performed to assess the changes between T1 and T2. Results No statistically significant differences in molar inclination were observed between groups and timepoints (P > 0.05. There were no statistically significant differences in molar movement percentages (P > 0.05 irrespective of whether fixed retention had been used or not. Conclusions No significant eruption occurred in unopposed mandibular second molars bonded with fixed sectional retainers compared to molars partially occluded with the antagonists without fixed retention. Given the study limitations, fixed retention should be considered with caution in restricting tooth overeruption in unopposed molars.

  3. Does fixed retention prevent overeruption of unopposed mandibular second molars in maxillary first molar extraction cases?

    NARCIS (Netherlands)

    Livas, Christos; Halazonetis, Demetrios J; Booij, Johan W; Katsaros, Christos; Ren, Yijin

    2016-01-01

    BACKGROUND: The objective of this study was to investigate whether multistranded fixed retainers prevented overeruption of unopposed mandibular second molars in maxillary first molar extraction cases. METHODS: The panoramic radiographs of 65 Class II Division 1 Caucasian Whites (28 females, 37 males

  4. Kissing molars extraction: Case series and review of the literature.

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    Arjona-Amo, Manuel; Torres-Carranza, Eusebio; Batista-Cruzado, Antonio; Serrera-Figallo, Maria-Angeles; Crespo-Torres, Santos; Belmonte-Caro, Rodolfo; Albisu-Andrade, Claudio; Torres-Lagares, Daniel; Gutiérrez-Pérez, José-Luis

    2016-02-01

    Kissing molars are a very rare form of inclusion defined as molars included in the same quadrant, with occlusal surfaces contacting each other within a single dental follicle. We present four cases of this pathology: a 35 year-old male, referred to the Oral and Maxillofacial Surgery Department of the Hospital Virgen del Rocio in Seville, and three females of 24, 26, and 31 years, all of which had kissing molars that were treated by tooth extraction. We have found only 10 cases published in the medical literature in which this type of inclusion is briefly described, none of which elaborate on the surgical technique employed. In these cases, the indication for surgery is established when there is a history of recurring infections or cystic lesions associated with dental inclusions. The extraction of kissing molars requires an exhaustive comprehension of the anatomy of the region involved, sufficiently developed surgical abilities, and an extensive planning process. Impacted molar, kissing molar, surgical extraction.

  5. Effect of socket preservation therapies following tooth extraction in non-molar regions in humans: a systematic review

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    ten Heggeler, J.M.A.G.; Slot, D.E.; van der Weijden, G.A.

    2011-01-01

    Objective: To assess, based on the existing literature, the benefit of socket preservation therapies in patients with a tooth extraction in the anterior or premolar region as compared with no additional treatment with respect to bone level. Material and methods: MEDLINE-PubMed and the Cochrane

  6. Effect of socket preservation therapies following tooth extraction in non-molar regions in humans: a systematic review

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    ten Heggeler, J.M.A.G.; Slot, D.E.; van der Weijden, G.A.

    2011-01-01

    Objective: To assess, based on the existing literature, the benefit of socket preservation therapies in patients with a tooth extraction in the anterior or premolar region as compared with no additional treatment with respect to bone level. Material and methods: MEDLINE-PubMed and the Cochrane Centr

  7. The orthodontic extraction of permanent molars: a literature review.

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    Chua, Emilia S L; Felicita, A Sumathi

    2015-05-01

    The most common cause of dental crowding is the presence of an arch-length--tooth-size discrepancy. Conventional methods of gaining space in orthodontics involve the extraction of teeth, often premolars. However, there are a number of clinical situations in which the extraction of permanent molars might be considered. This paper highlights the indications, advantages, disadvantages and timing of the extraction of the first, second and third permanent molars in the treatment of a crowded malocclusion.

  8. Anxiety before extraction of impacted lower third molars

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    Tarazona, Beatriz; Tarazona-Álvarez, Pablo; Peñarrocha-Oltra, David; Rojo-Moreno, Juan; Peñarrocha-Diago, Maria

    2015-01-01

    Assess levels of trait anxiety, state anxiety and dental anxiety before extraction of lower third molars and check the correlation and reliability of the scales used for the measurement of preoperative anxiety...

  9. Anxiety before extraction of impacted lower third molars

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    Tarazona Álvarez, Beatriz; Tarazona Álvarez, Pablo; Peñarrocha Oltra, David; Rojo Moreno, Juan; Peñarrocha Diago, María

    2015-01-01

    Objetives: Assess levels of trait anxiety, state anxiety and dental anxiety before extraction of lower third molars and check the correlation and reliability of the scales used for the measurement of preoperative anxiety. Study Design: A prospective study of patients treated with extraction of a lower third molar between September 2010 to December 2010 was carried out. A total of 125 patients were included in the study. All of them were patients of the Oral Surgery and Implantology Department...

  10. Influence of unilateral maxillary first molar extraction treatment on second and third molar inclination in Class II subdivision patients

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    Livas, Christos; Pandis, Nikolaos; Booij, Johan Willem; Halazonetis, Demetrios J.; Katsaros, Christos; Ren, Yijin

    2016-01-01

    Objective: To assess the maxillary second molar (M2) and third molar (M3) inclination following orthodontic treatment of Class II subdivision malocclusion with unilateral maxillary first molar (M1) extraction. Materials and Methods: Panoramic radiographs of 21 Class II subdivision adolescents (eight

  11. Anxiety and extraction of third molars in Turkish patients.

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    Garip, Hasan; Abali, Osman; Göker, Kamil; Göktürk, Ulkü; Garip, Yildiz

    2004-12-01

    Preoperative anxiety is widespread and adversely affects a patient's physical and psychological outcome. Extraction of third molars is common, and many patients complain of anxiety and emotional disturbance. We assessed the anxiety of patients in Turkey before extraction of third molars. A total of 120 patients were admitted for removal of one or more third molars under local anaesthesia. The Amsterdam Preoperative Anxiety and Information Scale (APAIS) and Spielberger's State Trait Anxiety Inventory (STAI) were used to evaluate anxiety. The results showed that women were significantly more anxious than men; women who had not had a previous operation were more anxious than other women; there was no difference in the anxiety scores of patients who had previously had a local anaesthetic and those who had not; there were no differences in anxiety as measured by trait scores; patients who wanted a lot of information were more anxious.

  12. Extrações de molares na Ortodontia Molar extractions in orthodontics

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    Marco Antônio Schroeder

    2011-12-01

    Full Text Available O tratamento ortodôntico com extração de molares em pacientes adultos é tecnicamente mais complexo, devido a inúmeros fatores. Em geral, o espaço a ser fechado é maior do que o espaço dos pré-molares e, por isso, a ancoragem é crítica e o tempo de tratamento mais longo. É comum esses casos apresentarem algum grau de comprometimento periodontal por causa da idade dos pacientes e, portanto, necessitam de maior controle da mecânica ortodôntica para reduzir os efeitos colaterais do fechamento do espaço. Por isso, bons resultados de finalização são mais difíceis de ser alcançados. Sendo assim, este artigo tem como objetivo apresentar as razões para a indicação de extração de molares nos tratamentos ortodônticos, as contraindicações, as diferentes fases da mecânica ortodôntica, esclarecer os fatores envolvidos nesse tipo de planejamento e tratamento e apresentar casos clínicos tratados com extração de molares.Orthodontic treatment with extraction of molars in adult patients is technically more complex due to a number of factors. In general, the space to be closed is greater than premolar spaces rendering critical anchorage and longer treatment time. Often such cases exhibit some degree of periodontal involvement due to patient age. Hence, the need for greater control over orthodontic mechanics to reduce the side effects of space closure. Therefore, good finishing results can be more difficult to achieve. Thus, the purpose of this article is to determine the reasons for molar extraction indications, describe different stages of orthodontic mechanics, and explain the issues involved in this kind of planning and treatment. Additionally, it aims at describing some treatments with molar extractions.

  13. DEEP NECK INFECTION AFTER THIRD MOLAR EXTRACTION*

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    Seda YILMAZ

    2015-04-01

    Full Text Available Odontogenic and oropharyngeal infections are relatively common in the cervicofacial region. In rare cases, odontogenic or peritonsillar abscesses may spread through the deep fascial cervical spaces and cause life-threatening complications. Odontogenic infection is the most common cause of deep neck infections and it accounts for 43% of the cases. Early diagnosis, immediate antibiotic treatment, and surgical drainage are the basis of therapeutic success. Deep neck infections are potentially life threatening complications if they are not diagnosed in time and treated quickly. This case report presents clinical, radiological features and treatment of the spread of abscesses through cervical spaces of an unusual case of deep neck infection that was caused by the secondary infection of the root remnants after extraction.

  14. Piezoelectric Versus Conventional Rotary Techniques for Impacted Third Molar Extraction

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    Jiang, Qian; Qiu, Yating; Yang, Chi; Yang, Jingyun; Chen, Minjie; Zhang, Zhiyuan

    2015-01-01

    Abstract Impacted third molars are frequently encountered in clinical work. Surgical removal of impacted third molars is often required to prevent clinical symptoms. Traditional rotary cutting instruments are potentially injurious, and piezosurgery, as a new osteotomy technique, has been introduced in oral and maxillofacial surgery. No consistent conclusion has been reached regarding whether this new technique is associated with fewer or less severe postoperative sequelae after third molar extraction. The aim of this study was to compare piezosurgery with rotary osteotomy techniques, with regard to surgery time and the severity of postoperative sequelae, including pain, swelling, and trismus. We conducted a systematic literature search in the Cochrane Library, PubMed, Embase, and Google Scholar. The eligibility criteria of this study included the following: the patients were clearly diagnosed as having impacted mandibular third molars; the patients underwent piezosurgery osteotomy, and in the control group rotary osteotomy techniques, for removing impacted third molars; the outcomes of interest include surgery time, trismus, swelling or pain; the studies are randomized controlled trials. We used random-effects models to calculate the difference in the outcomes, and the corresponding 95% confidence interval. We calculated the weighted mean difference if the trials used the same measurement, and a standardized mean difference if otherwise. A total of seven studies met the eligibility criteria and were included in our analysis. Compared with rotary osteotomy, patients undergoing piezosurgery experienced longer surgery time (mean difference 4.13 minutes, 95% confidence interval 2.75–5.52, P trismus in the piezosurgery groups. The number of included randomized controlled trials and the sample size of each trial were relatively small, double blinding was not possible, and cost analysis was unavailable due to a lack of data. Our meta-analysis indicates that although

  15. Changes in the Position of Mandibular Third Molars Following Extraction and Non-Extraction Orthodontic Treatments

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    Maryam Poosti

    2013-01-01

    Full Text Available Introduction: Impaction of third molars can be associated with a number of sequelae, each requiring a different type of treatment. The purpose of this study was to assess the changes in the position of lower third molars following extraction and non-extraction orthodontic treatments. Methods: Pre- and post-treatment panoramic radiographs of 26 class I malocclusion patients (4 male and 22 female who were treated on a non-extraction basis were compared with those of 30 class I malocclusion patients (5 male and 25 female who received orthodontic treatment following the extraction of all first premolars. In each group, the lower third molar angulations and their distance from the lower border of the mandible were assessed. Results: Paired t-tests and independent t-tests detected significant changes in the position of the lower third molars in the extraction group. Both the left and right molars became more upright and their distance from the lower border of the mandible increased. Similar changes were only seen in the position of the right mandibular third molars in the non-extraction group. Conclusion: Tooth extractions can bring about favorable changes in the position of mandibular third molars and possibly reduce their incidence of impaction

  16. Changes in the Position of Mandibular Third Molars Following Extraction and Non-Extraction Orthodontic Treatments

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    Maryam Poosti

    2012-09-01

    Full Text Available Introduction: Impaction of third molars can be associated with a number of sequelae, each requiring a different type of treatment. The purpose of this study was to assess the changes in the position of lower third molars following extraction and non-extraction orthodontic treatments. Methods: Pre- and post-treatment panoramic radiographs of 26 class I malocclusion patients (4 male and 22 female who were treated on a non-extraction basis were compared with those of 30 class I malocclusion patients (5 male and 25 female who received orthodontic treatment following the extraction of all first premolars. In each group, the lower third molar angulations and their distance from the lower border of the mandible were assessed. Results: Paired t-tests and independent t-tests detected significant changes in the position of the lower third molars in the extraction group. Both the left and right molars became more upright and their distance from the lower border of the mandible increased. Similar changes were only seen in the position of the right mandibular third molars in the non-extraction group. Conclusion: Tooth extractions can bring about favorable changes in the position of mandibular third molars and possibly reduce their incidence of impaction.

  17. Changes in the Position of Mandibular Third Molars Following Extraction and Non-Extraction Orthodontic Treatments

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    Maryam Poosti; Mohammad Basafa; Mohsen Hosseini; Farnaz Parvizi

    2012-01-01

    Introduction: Impaction of third molars can be associated with a number of sequelae, each requiring a different type of treatment. The purpose of this study was to assess the changes in the position of lower third molars following extraction and non-extraction orthodontic treatments. Methods: Pre- and post-treatment panoramic radiographs of 26 class I malocclusion patients (4 male and 22 female) who were treated on a non-extraction basis were compared with those of 30 class I malocclusion pat...

  18. Anxiety before extraction of impacted lower third molars.

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    Tarazona, Beatriz; Tarazona-Álvarez, Pablo; Peñarrocha-Oltra, David; Rojo-Moreno, Juan; Peñarrocha-Diago, Maria

    2015-03-01

    Assess levels of trait anxiety, state anxiety and dental anxiety before extraction of lower third molars and check the correlation and reliability of the scales used for the measurement of preoperative anxiety. A prospective study of patients treated with extraction of a lower third molar between September 2010 to December 2010 was carried out. A total of 125 patients were included in the study. All of them were patients of the Oral Surgery and Implantology Department (Valencia University Medical and Dental School, Valencia, Spain). Before surgery, patients had to complete a preoperative protocol with 4 scales: the STAI-T (State-Trait Anxiety Inventory-Trait) for measuring trait anxiety, the STAI-S (State-Trait Anxiety Inventory-State) for measuring state anxiety, and DAS (Dental anxiety Scale of N. Corah) and APAIS (Amsterdam Preoperative anxiety and Information Scale) for measuring dental anxiety. Patients undergoing extractions of an impacted lower third molar showed low levels of trait anxiety and moderate levels of state anxiety and dental anxiety. Higher levels of trait anxiety were obtained for older patients. Women had higher mean levels of dental anxiety and state anxiety that men with a statistically significant difference in STAI-S scales, DAS, and APAIS. Patients with higher trait anxiety and state anxiety showed higher levels of dental anxiety. A significant correlation (p ≤ 0.01) (p = 0.00) was found between the four scales used to measure anxiety. The scale showed higher correlation was STAI-S scale. The 4 scales showed high reliability (α of C.> 0.80). Patients with highest levels of trait anxiety and state anxiety, had more dental anxiety. The STAI-T, STAI-S, DAS and APAIS scales provided useful information about anxiety before the extraction of lower impacted third molars. The STAI-S is the scale with highest correlation and reliability.

  19. Extraction of maxillary first molars improves second and third molar inclinations in Class II Division 1 malocclusion

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    Livas, C.; Halazonetis, D.J.; Booij, J.W.; Katsaros, C.

    2011-01-01

    INTRODUCTION: The aim of this study was to assess the changes in inclination of the maxillary second (M2) and third (M3) molars after orthodontic treatment of Class II Division 1 malocclusion with extraction of maxillary first molars. METHODS: Two groups of subjects were studied. The experimental gr

  20. Extraction of maxillary first molars improves second and third molar inclinations in Class II Division 1 malocclusion

    NARCIS (Netherlands)

    Livas, C.; Halazonetis, D.J.; Booij, J.W.; Katsaros, C.

    2011-01-01

    INTRODUCTION: The aim of this study was to assess the changes in inclination of the maxillary second (M2) and third (M3) molars after orthodontic treatment of Class II Division 1 malocclusion with extraction of maxillary first molars. METHODS: Two groups of subjects were studied. The experimental

  1. Dentinal innervation of impacted human third molars.

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    Lilja, J; Fagerberg-Mohlin, B

    1984-12-01

    Five totally impacted third molars were studied in the transmission electron microscope for the presence of nervous structures in the dentin before eruption. In contradiction to earlier studies available, nervous structures were found in the predentin and the dentin of the impacted third molars in different parts of the crown and also in the predentin of the root.

  2. Peripheral facial nerve paralysis after upper third molar extraction.

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    Cakarer, Sirmahan; Can, Taylan; Cankaya, Burak; Erdem, Mehmet Ali; Yazici, Sinem; Ayintap, Emre; Özden, Ali Veysel; Keskin, Cengizhan

    2010-11-01

    Peripheral facial nerve paralysis (PFNP) after mandibular interventions has been reported in the literature. In most cases, paralysis begins immediately after the injection of the mandibular anesthesia, and duration of facial weakness is less than 12 hours. However, there are few documented cases of PFNP after maxillary dental or surgical procedures. A variety of mechanisms have been associated to PFNP, including viral reactivation, demyelination, edema, vasospasm, and trauma. The purpose of this presentation was to report a rare case of facial paralysis that occurred after an upper third molar extraction. The cause of the PFNP and the importance of the multidisciplinary approach in the management are emphasized.

  3. Platelet-rich plasma in periodontal defect treatment after extraction of impacted mandibular third molars

    National Research Council Canada - National Science Library

    Moghe, Swapnil; Saini, Nitin; Moghe, Anjali

    2012-01-01

    .... Platelet-rich plasma (PRP) is a material containing many autologous growth factors that may be used in repairing and preventing periodontal complications at the distal root of the second molar adjacent to the extracted third molar...

  4. Pulpotomies with portland cement in human primary molars

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    Taísa Regina Conti

    2009-02-01

    Full Text Available Two clinical cases in which Portland cement (PC was applied as a medicament after pulpotomy of mandibular primary molars in children are presented. Pulpotomy using PC was carried out in two mandibular first molars and one mandibular second molar, which were further followed-up. At the 3, 6 and 12-month follow-up appointments, clinical and radiographic examinations of the pulpotomized teeth and their periradicular area revealed that the treatments were successful in maintaining the teeth asymptomatic and preserving pulpal vitality. Additionally, the formation of a dentin bridge immediately below the PC could be observed in the three molars treated. PC may be considered as an effective alternative for primary molar pulpotomies, at least in a short-term period. Randomized clinical trials with human teeth are required in order to determine the suitability of PC before unlimited clinical use can be recommended.

  5. MTA pulpotomy of human permanent molars with irreversible pulpitis.

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    Eghbal, Mohammad Jafar; Asgary, Saeed; Baglue, Reza Ali; Parirokh, Masoud; Ghoddusi, Jamileh

    2009-04-01

    The histological success of mineral trioxide aggregate (MTA) pulpotomy for treatment of irreversible pulpitis in human teeth as an alternative treatment was investigated in this study. Fourteen molars which had to be extracted were selected from patients 16-28 years old. The selection criteria include carious pulp exposure with a history of lingering pain. After isolation, caries removal and pulp exposure, MTA was used in pulpotomy treatment. Patients were evaluated for pain after 24 h. Two patients were lost from this study. Twelve teeth were extracted after 2 months and were assessed histologically. Recall examinations confirmed that none of the patients experienced pain after pulpotomy. Histological observation revealed that all samples had dentin bridge formation completely and that the pulps were vital and free of inflammation. Although the results favour the use of MTA as a pulpotomy material, more studies with larger samples and a longer recall period are suggested to justify the use of MTA for treatment of irreversible pulpitis in human permanent teeth.

  6. Does Psychological Profile Influence Third Molar Extraction and Postoperative Pain?

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    González-Martínez, Raquel; Jovani-Sancho, María Del Mar; Cortell-Ballester, Isidoro

    2017-03-01

    Our purposes were to determine the influence of psychological profile on hemodynamic changes in patients who undergo surgical removal of the third molars under intravenous sedation and to evaluate the effect on patients' anxiety and postoperative recovery. We performed a prospective study of 100 patients (American Society of Anesthesiologists classes I and II; aged ≥18 years) seen in the CIMIVClinic (Department of Oral Surgery, Casa de Salud University Hospital, Valencia, Spain) who underwent extractions of all third molars under intravenous sedation. All patients were administered the Symptom Checklist 90 Revised (SCL-90-R). The following parameters were monitored at different times during the surgical interventions: systolic blood pressure, diastolic blood pressure, oxygen saturation, and heart rate. Position and depth of impaction of the tooth (Pell and Gregory classification and Winter classification), surgery duration, and surgical technique also were recorded. Finally, the degree of pain experienced the week after the surgical intervention was measured using a visual analog scale. Patients' anxiety levels preoperatively were significantly higher in patients with psychological distress (P = .023). Postoperative pain significantly decreased from the first day to the seventh day in healthy patients but not in patients with altered psychological conditions (P < .05). Nevertheless, the hemodynamic changes were not correlated with the psychological impairment. Intravenous sedation enables the control of hemodynamic changes in all patients independently of their psychological profile. Patients with psychological distress present with higher levels of dental anxiety and postoperative pain. Future studies are needed to further clarify this interaction. Copyright © 2016 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  7. Comparison of platelet rich plasma and synthetic graft material for bone regeneration after third molar extraction

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    Nathani, Dipesh B.; Sequeira, Joyce; Rao, B. H. Sripathi

    2015-01-01

    Aims: To compare the efficacy of Platelet rich plasma and synthetic graft material for bone regeneration after bilateral third molar extraction. Material and Methods: This study was conducted in 10 patients visiting the outpatient department of Oral & Maxillofacial Surgery, Yenepoya Dental College & Hospital. Patients requiring extraction of bilateral mandibular third molars were taken for the study. Following extraction, PRP (Platelet Rich Plasma) was placed in one extraction socket and synt...

  8. Alveolar Osteitis: Patients′ compliance to post-extraction instructions following extraction of molar teeth

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    Osagie Akpata

    2013-01-01

    Full Text Available Background: To evaluate the effect of various combination of post-extraction regimen administered to patients who had intra-alveolar molar tooth extraction. Patients and Methods: One year prospective study involving 76 consenting patients who came for 1-week post-extraction review. The patients were placed on warm saline mouth rinse with (verbal instruction or without antibiotic and or analgesic therapy (written prescription, after intra-alveolar molar tooth extraction. Information was obtained from the patients through questionnaire and clinical examination. Results: The patients were placed on warm saline mouth rinse (n = 29, 38.2% only, warm saline rinse, antibiotics (Amoxicillin and metronidazole and Paracetamol (n = 31, 40.8%, Paracetamol and warm saline rinse (n = 12, 15.8% and antibiotics (Amoxicillin and metronidazole and Paracetamol (n = 4, 5.3%. A total of 63 (82.9% patients complied with the post-extraction regimen, giving a significant high compliance to the post-extraction instructions (P = 0.001. There were 10 (13.2% cases of post-extraction localised alveolar osteitis, with predilection for the lower molar teeth (n = 6, 7.9% and a significant predilection for females (n = 8, 10.5% [P = 0.005]. Overall, there were five (6.6% cases each of localised alveolar osteitis in the compliant patients (n = 63, 82.9% and non-compliant patients (n = 13, 17.1%, giving a ratio of 1:13 and 1:3, respectively. There was significant association of compliance with post-extraction instruction and the reduced incidence of localized alveolar osteitis (P = 0.015. Conclusion: This study showed a significant patients′ compliance with post-extraction warm saline rinse, prophylactic antibiotics and analgesic and a corresponding significant reduction in the incidence of localised alveolar osteitis following intra-alveolar molar tooth extraction. This study emphasises the need to properly educate patients on the effect of compliance to various combination of

  9. Maxillary sinus pneumatization after maxillary molar extraction assessed with cone beam computed tomography

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    Jung, Yu Hoa; Nah, Kyung Soo; Cho, Bong Hae [Department of Oral and Maxillofacial Radiology, College of Dentistry, Pusan National University, Pusan (Korea, Republic of)

    2009-09-15

    The purpose of this study was to examine the inferior expansion of the maxillary sinus floor following maxillary molar extraction. Cone beam computed tomographic images of 59 subjects were used to evaluate the height difference of the maxillary sinus floor between extraction sites and contralateral dentate sites. The height of the maxillary sinus floor was defined as the vertical distance to the Frankfort plane from the level of the anterior nasal spine to the most inferior point of the sinus floor. We examined the difference in sinus pneumatization according to the number of missing teeth and the vertical relationship of the molar roots to the sinus floor. The inferior expansion of the maxillary sinus floor was 1.20 {+-} 1.86 mm on the maxillary first molar and 1.90 {+-} 2.42 mm on the maxillary second molar. Increased expansion was observed in cases where two proximate molars were extracted. There was no significant difference in sinus pneumatization following extraction according to the vertical relationship of the molar roots to the sinus floor. The results of this study confirm that sinus pneumatization occurs following maxillary molar extraction. In situations where pneumatization can affect treatment after molar extraction, three-dimensional radiography should be considered.

  10. EXTRACTION, QUANTIFICATION, AND MOLAR MASS DETERMINATION OF HYALURONIC ACID EXTRACTED FROM CHICKEN CREST

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    C. S. ROSA

    2008-11-01

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    Hyaluronic acid (HA is part of the connective tissue. The polymer is composed of alternating units of ß-d-glucuronic acid and N-acetyl-ß-d-glucosamine linked, respectively, via 1-3 and 1-4 bonds. The chicken crest is one of the richest tissues in this polysaccharide. Since Brazil is one of the main chicken exporters in the world, the utilization of the crests of abated animals for the HA obtaining is particularly attractive. The present work sought to extract HA from chicken crest and to determine the molar mass of the extracted acid. Extraction was accomplished by proteolytic digestion with papain during 24 h at 60oC, followed by precipitation with cetylpyridinium chloride (CPC. Hexuronic acid content was determined via the carbazole method, the intrinsic viscosity was measured using the ball viscosimeter, and the molar mass was calculated by extrapolating the calibration line to zero. In addition, qualitative infrared spectroscopy was carried out on the sample using the Bomem MB spectrophotometer. The results show that the extraction method was effective: the extracted acid possesses a large molecular mass, and the extract contains a signifi cant amount of HA.

  11. Indications for extraction of third molars: a review of 1763 cases.

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    Adeyemo, W L; James, O; Ogunlewe, M O; Ladeinde, A L; Taiwo, O A; Olojede, A C O

    2008-03-01

    Third molars have been described as different from other teeth in the oral cavity. They have the highest rate of developmental abnormalities and, most importantly, are last in the eruption sequence. We investigated reasons for third molar teeth extraction in patients attending the outpatient oral surgery clinic of the Lagos University Teaching Hospital, Nigeria. A retrospective review of patients who had third molar extractions between January 2001 and June 2006 was conducted. Data retrieved and analysed were: age and sex of patients, indication for extractions, type of teeth extracted, technique of extractions (surgical and non-surgical) and angulations of impaction in case of impacted lower third molars. A total of 1763 patients (mean age +/-SD, 33.74+/- 13.3 years; range 15 - 92 years) had their third molars extracted during the period. Majority (58%) of them were females. Surgical extraction was carried out in 506 (28.7%) patients while 1257 (71.3%) patients had non-surgical extractions. Caries and its sequela (63.2%) was the major reason for teeth extraction followed by recurrent pericoronitis (26.3%) and periodontitis (9.2%). Only 11 (0.6%) cases were removed for prophylactic reason. Patients who had their teeth removed for caries were significantly younger than those for periodontitis (P=0.000) but older than those for pericoronitis (P=0.000). Caries and periodontal diseases occurring in relatively older age group were the major reasons for non-surgical extraction of third molars while recurrent pericoronitis occurring in relatively younger age was the major reason for surgical extraction of impacted third molars. Prophylactic surgical extraction of third molars is not a common practice in our environment.

  12. Which factors are associated with difficult surgical extraction of impacted lower third molars?

    OpenAIRE

    Park, Kyeong-Lok

    2016-01-01

    Objectives The aim of this retrospective study was to investigate factors associated with increased difficulty in the surgical extraction of impacted lower third molars and to improve identification of difficult cases. Materials and Methods A total of 680 patients who required 762 surgical extractions of impacted lower third molars from 2009 to 2014 were enrolled in the study. Demographic factors, clinical factors, radiographic factors, surgical extraction difficulty, and presumed causes of d...

  13. COMPLICATIONS AFTER EXTRACTION OF IMPACTED THIRD MOLARS - LITERATURE REVIEW

    OpenAIRE

    Elitsa G. Deliverska; Milena Petkova

    2016-01-01

    Third molar surgery is the most common procedure performed by oral and maxillofacial surgeons worldwide. This article addresses the incidence of specific complications and, where possible, offers a preventive or management strategy. Complications, such as pain, dry socket, swelling, paresthesia of the lingual or inferior alveolar nerve, bleeding, and infection are most common. Factors thought to influence the incidence of complications after third molar removal include age, gender, medical hi...

  14. COMPLICATIONS AFTER EXTRACTION OF IMPACTED THIRD MOLARS - LITERATURE REVIEW

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    Elitsa G. Deliverska

    2016-07-01

    Full Text Available Third molar surgery is the most common procedure performed by oral and maxillofacial surgeons worldwide. This article addresses the incidence of specific complications and, where possible, offers a preventive or management strategy. Complications, such as pain, dry socket, swelling, paresthesia of the lingual or inferior alveolar nerve, bleeding, and infection are most common. Factors thought to influence the incidence of complications after third molar removal include age, gender, medical history, oral contraceptives, presence of pericoronitis, poor oral hygiene, smoking, type of impaction, relationship of third molar to the inferior alveolar nerve, surgical time, surgical technique, surgeon experience, use of perioperative antibiotics, use of topical antiseptics, use of intra-socket medications, and anaesthetic technique. For the general dental practitioner, as well as the oral and maxillofacial surgeon, it is important to be familiar with all the possible complications after this procedure. This improves patient education and leads to prevention, early recognition and management.

  15. Capacidade seladora proporcionada por alguns materiais quando utilizados em perfurações na região de furca de molares humanos extraídos Sealing capacity produced by some materials when utilized under furcation perforations of extract human molars

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    Ulisses Xavier da Silva Neto

    2003-03-01

    Full Text Available Avaliou-se, in vitro, a capacidade seladora, do MTA-Angelus, ProRoot-MTA, Super-EBA e MBP-c, quando utilizados para selar perfurações na região de furca de molares humanos extraídos; quando aplicados isoladamente ou em associação com uma matriz de gesso Paris, aplicada no fundo do trajeto da perfuração. O selamento produzido pelos materiais foi analisado por meio da infiltração do corante rhodamine B 0,2%. A análise estatística aplicada aos dados obtidos demonstrou que, quando utilizado isoladamente (sem matriz, o cimento MBP-c apresentou os menores índices de infiltração marginal, seguido pelo Super-EBA, apresentando diferença estatística significante (pIt has been evaluated in vitro the sealing capacity of MTA-Angelus, ProRoot-MTA, Super-EBA and MBP-c when sealing furcal perforations of extracted human molars. The experiment took place with materials both isolated as well as in association with a plaster of Paris matrix applied at the bottom of the perfuration path. The materials sealing capacity was analyzed by dye leakage of rhodamine B 0,2%.The statistical analysis revealed that when isolated and tested (without the matrix, the cement MBP-c presented the smallest coefficient of marginal leakage, followed by the Super-EBA. There was a statistical significance between those and the cements ProRoot - MTA e MTA - Angelus (p<0,001. In the presence of the matrix, the cement MBP-c also had a superior performance having statistical significance between those and the cements ProRoot - MTA e MTA - Angelus (p<0,05; The Plaster of Paris matrix had a negative impact with statistical significance regarding the sealing capacity of the cements Super-EBA and the MBP-c (p<0,05. Nevertheless, it avoided the leakage of the respective sealing materials.

  16. Stability of molar relationship after non-extraction Class II malocclusion treatment

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    Darwin Vaz de Lima

    2013-04-01

    Full Text Available OBJECTIVE: This study aimed to evaluate the stability of molar relationship after non-extraction treatment of Class II malocclusion. METHODS: The sample comprised 39 subjects (16 females, 23 males with initial Class II malocclusion treated with no extractions, using fixed appliances. Mean age at the beginning of treatment was 12.94 years, at the end of treatment was 15.14 years and at post-retention stage was 21.18 years. Mean treatment time was 2.19 years and mean time of post-treatment evaluation was 6.12 years. To verify the influence of the severity of initial Class II molar relationship in stability of molar relationship, the sample was divided into two groups, one presenting a ½-cusp or ¾-cusp Class II molar relationship, and the other with full-cusp Class II molar relationship. In dental casts from initial, final and postretention stages, molar, first and second premolars and canine relationships were measured. Data obtained were analyzed by dependent ANOVA, Tukey and Pearson's correlation tests, as well as independent t test between the two groups divided by severity of initial molar relationship. RESULTS: There was a non-statistically significant 0.12 mm relapse of molar relationship. The initial severity of Class II molar relationship was not correlated to relapse in the post-retention period. When compared, the two groups showed no difference in relapse of molar relationship. CONCLUSION: It was concluded that correction of Class II molar relationship is stable and initial severity does not influence relapse of molar relationship.

  17. [Orthodontic partial disimpaction of mandibular third molars prior to surgical extraction].

    Science.gov (United States)

    Derton, Nicolà; Perini, Alessandro; Giordanetto, José; Biondi, Giovanni; Siciliani, Giuseppe

    2009-06-01

    Odontodysplasia of the third molars is a relatively common anomaly. The frequent complications associated with this disorder very often constitute an indication for extraction of the third molar. This surgical treatment can damage the lower alveolar nerve and/or trigger distal bone loss of the second molar, thus jeopardizing the future status of the periodontium. The author presents two case studies treated exclusively with miniscrews with no dental anchorage in order to achieve partial eruption of the third molar moving it away from the lower alveolar nerve and to avoid unwanted impact on other teeth. Following this procedure, the third molar was extracted without complications. In conclusion, this approach can offer an alternative to surgical treatment alone in cases where the proximity of tooth and nerve poses a significant risk.

  18. Unilateral Maxillary First Molar Extraction in Class II Subdivision: An Unconventional Treatment Alternative

    OpenAIRE

    J. W. Booij; Christos Livas

    2016-01-01

    The asymmetrical intra-arch relationship in Class II subdivision malocclusion poses challenges in the treatment planning and mechanotherapy of such cases. This case report demonstrates a treatment technique engaging unilateral extraction of a maxillary first molar and Begg fixed appliances. The outcome stability and the enhancing effect on the eruption of the third molar in the extraction segment were confirmed by a 4-year follow-up examination.

  19. Unilateral Maxillary First Molar Extraction in Class II Subdivision: An Unconventional Treatment Alternative

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    J. W. Booij

    2016-01-01

    Full Text Available The asymmetrical intra-arch relationship in Class II subdivision malocclusion poses challenges in the treatment planning and mechanotherapy of such cases. This case report demonstrates a treatment technique engaging unilateral extraction of a maxillary first molar and Begg fixed appliances. The outcome stability and the enhancing effect on the eruption of the third molar in the extraction segment were confirmed by a 4-year follow-up examination.

  20. Recurrent condylar luxation after third molar extraction: an uncommon condition treated by eminectomy

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    Maiolino Thomaz Fonseca OLIVEIRA

    Full Text Available INTRODUCTION: Extraction of third molars can rarely provoke post-operative complications, such as temporomandibular joint disorders (TMDs. OBJECTIVE: Although the literature presents a series of discussions of the clinical evidence related to this subject, in this report, we present an uncommon case of a patient submitted for the extraction of third molars who presented, in postoperative monitoring, with recurrent bilateral condylar luxation. CONCLUSION: Due to this critical condition, the patient was successfully treated by bilateral eminectomy.

  1. A randomized comparative prospective study of platelet-rich plasma, platelet-rich fibrin, and hydroxyapatite as a graft material for mandibular third molar extraction socket healing

    OpenAIRE

    Dutta, Shubha Ranjan; Passi, Deepak; Singh, Purnima; Sharma, Sarang; Singh, Mahinder; Srivastava, Dhirendra

    2016-01-01

    Aim: The purpose of this study was to compare the efficacy of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and hydroxyapatite (HA) for reduction of pain and swelling, absence of dry socket, soft tissue healing, and bone regeneration after mandibular third molar extraction in human patients. Materials and Methods: Forty patients requiring extraction of mandibular third molars were randomly grouped as control, PRP, PRF, and HA-treated. The patients were assessed for postoperative pai...

  2. Horizontal and vertical changes in anchor molars after extractions in bimaxillary protrusion cases

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    Pratik Chandra

    2016-01-01

    Full Text Available Objective: To evaluate changes in the anchor molar position (horizontal, vertical after retraction in bimaxillary protrusion maximum anchorage cases. Materials and Methods: Thirty patients requiring maximum anchorage after extraction of the first premolars were selected for this study. The second molars were banded in both arches along with trans-palatal arch in the maxillary arch and lingual arch in the mandibular arch. En mass retraction was done using sliding mechanics. Horizontal and vertical positions of the anchor first molars were evaluated cephalometrically before and after orthodontic retraction. Results: In the horizontal plane, maxillary first molars showed net mesial movement of 1.72 mm, and there was a statistical difference between the pre- and post-values (P < 0.001. The mandibular molars showed a net horizontal movement of 2.26 mm, and there was a statistically significant difference between the pre- and post-values (P < 0.001. In the vertical plane, there was vertical movement of the maxillary anchor molars by a net value of 0.95 mm which was statistically significant (P < 0.001. The mandibular anchor molars moved vertically by a net value of 0.45 mm. This difference was statistically not significant. Conclusion: There was anchorage loss seen in both the planes (horizontal, vertical of the maxillary anchor molars. In the mandibular anchor molars, there was anchorage loss seen only in the horizontal plane. No anchorage loss was seen in the vertical plane.

  3. Early extraction: a silver bullet to avoid nerve injury in lower third molar removal?

    Science.gov (United States)

    Zhang, Q-B; Zhang, Z-Q

    2012-10-01

    This retrospective study evaluated the effects of early extraction of immature lower third molar on preventing complications, particularly nerve injury following lower third molar removal. Patients were grouped according to age and radiographic results: group A (518 patients, ≤23 years, immature teeth with apical foramen not closed); group B (532 patients, >23 years, mature teeth with closed apical foramen). Group A included 230 males and 288 females (average age 17 years). In group A, 808 lower mandibular third molars were extracted bilaterally in 290 and unilaterally in 228 patients; the incidence of complications was 2.48% (20/808) (all were temporary), the incidence of nerve injury was 0%. Group B included 250 males and 282 females (average age 39 years). In group B, 810 lower third molars were extracted bilaterally in 278 and unilaterally in 254 patients; the incidence of complications was 10% (81/810), the incidence of nerve injury was 1.6% (13/810). All complications were temporary, except two removals of permanent inferior alveolar nerve numbness (>6 months). In this study, early removal of the lower third molar was effective in avoiding some postoperative complications, especially nerve injury. Early extraction of lower third molar in youngsters is recommended following a team consultation.

  4. Predictive factors of difficulty in lower third molar extraction: A prospective cohort study.

    Science.gov (United States)

    Alvira-González, J; Figueiredo, R; Valmaseda-Castellón, E; Quesada-Gómez, C; Gay-Escoda, C

    2017-01-01

    Several publications have measured the difficulty of third molar removal, trying to establish the main risk factors, however several important preoperative and intraoperative variables are overlooked. A prospective cohort study comprising a total of 130 consecutive lower third molar extractions was performed. The outcome variables used to measure the difficulty of the extraction were operation time and a 100mm visual analogue scale filled by the surgeon at the end of the surgical procedure. The predictors were divided into 4 different groups (demographic, anatomic, radiographic and operative variables). A descriptive, bivariate and multivariate analysis of the data was performed. Patients' weight, the presence of bulbous roots, the need to perform crown and root sectioning of the lower third molar and Pell and Gregory 123 classification significantly influenced both outcome variables (pthird molars.

  5. Extraction the First Permanent Molar With Poor Prognosis in Mixed Dentition Period

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    B Seraj

    2002-02-01

    Full Text Available Whenever the first permanent molar is extracted or its long- term prognosis is poor, before taking any steps, a full clinical and radiographic evaluation associated with patients dental models investigation is necessary to determine the following cases:The quality and quantity of dentition, teeth missing, occlusion, buds position, orthodontic problems, the level of parents and patient cooperation for future long term orthodontic treatment and finally patient's oral hygiene. On the basis of this information, a decision is taken about the first permanent molar extraction with poor prognosis, either of balancing or compensatory type, especially when future orthodontic treatment is improbable. The aim of this article is to explain the principles of time and sequence of first permanent molar extraction.

  6. Patient anxiety and surgical difficulty in impacted lower third molar extractions: a prospective cohort study.

    Science.gov (United States)

    Aznar-Arasa, L; Figueiredo, R; Valmaseda-Castellón, E; Gay-Escoda, C

    2014-09-01

    Encountering patients who are fearful and anxious is common in dental practice and these factors can increase the complexity of dental procedures. A prospective cohort study was performed to assess whether patient anxiety influences the difficulty of impacted lower third molar extraction and to identify other predictive factors of surgical difficulty; 102 extractions done under local anaesthesia were assessed. Several preoperative variables were recorded (demographic, anatomical, and surgical) and patient anxiety was assessed through the use of various questionnaires. Extraction difficulty was measured using the operation time (OT) and a 100-mm visual analogue scale (difficulty VAS) completed by the surgeon. Patients with deep impacted third molars that required bone removal and tooth sectioning showed higher levels of preoperative anxiety. Significant correlations were found between questionnaire scores and the surgical difficulty (OT and difficulty VAS). OT was also related to age, depth of impaction, third molar angulations, proximity of the third molar roots to the mandibular canal, hard and soft tissue coverage, and the need to perform an ostectomy and tooth sectioning. Impacted lower third molar extraction is significantly more difficult in anxious patients. Other demographic, radiological, and surgical factors were also found to be significantly related to the surgical difficulty.

  7. Predictive factors of difficulty in lower third molar extraction: A prospective cohort study

    Science.gov (United States)

    Alvira-González, Joaquín; Valmaseda-Castellón, Eduard; Quesada-Gómez, Carmen; Gay-Escoda, Cosme

    2017-01-01

    Background Several publications have measured the difficulty of third molar removal, trying to establish the main risk factors, however several important preoperative and intraoperative variables are overlooked. Material and Methods A prospective cohort study comprising a total of 130 consecutive lower third molar extractions was performed. The outcome variables used to measure the difficulty of the extraction were operation time and a 100mm visual analogue scale filled by the surgeon at the end of the surgical procedure. The predictors were divided into 4 different groups (demographic, anatomic, radiographic and operative variables). A descriptive, bivariate and multivariate analysis of the data was performed. Results Patients’ weight, the presence of bulbous roots, the need to perform crown and root sectioning of the lower third molar and Pell and Gregory 123 classification significantly influenced both outcome variables (p< 0.05). Conclusions Certain anatomical, radiological and operative variables appear to be important factors in the assessment of surgical difficulty in the extraction of lower third molars. Key words:Third molar, surgical extraction, surgical difficulty. PMID:27918736

  8. Sharp mandibular bone irregularities after lower third molar extraction: Incidence, clinical features and risk factors

    Science.gov (United States)

    Alves-Pereira, Daniela; Valmaseda-Castellón, Eduard; Laskin, Daniel M.; Berini-Aytés, Leonardo; Gay-Escoda, Cosme

    2013-01-01

    Objectives: The purpose of this study was to determine the incidence and clinical symptoms associated with sharp mandibular bone irregularities (SMBI) after lower third molar extraction and to identify possible risk factors for this complication. Study Design: A mixed study design was used. A retrospective cohort study of 1432 lower third molar extractions was done to determine the incidence of SMBI and a retrospective case-control study was done to determine potential demographic and etiologic factors by comparing those patients with postoperative SMBI with controls. Results: Twelve SMBI were found (0.84%). Age was the most important risk factor for this complication. The operated side and the presence of an associated radiolucent image were also significantly related to the development of mandibular bone irregularities. The depth of impaction of the tooth might also be an important factor since erupted or nearly erupted third molars were more frequent in the SMBI group. Conclusions: SMBI are a rare postoperative complication after lower third molar removal. Older patients having left side lower third molars removed are more likely to develop this problem. The treatment should be the removal of the irregularity when the patient is symptomatic. Key words:Third molar, postoperative complication, bone irregularities, age. PMID:23524429

  9. Effect of orthodontic treatment involving first premolar extractions on mandibular third molar angulation and retromolar space

    Science.gov (United States)

    Vaillard-Jiménez, Esther; García-Rocha, Araceli; Bellot-Arcís, Carlos; Paredes-Gallardo, Vanessa

    2017-01-01

    Background Third molars present more problems than other teeth because they are the last teeth to erupt, and so it is important to assess their development when designing an orthodontic treatment plan. The aim of this study was to compare the angulation of the mandibular third molar and retromolar space before and after orthodontic treatment in cases involving first premolar extraction. Material and Methods 76 patients, 59 women (77.63%) and 17 men (22.36%), were recruited from the Orthodontics Clinic at Benemérita Universidad Autónoma de Puebla (Mexico). Panoramic radiographs were analyzed before and after orthodontic treatment that included first premolar extractions, measuring retromolar space (RS) and the angles formed by the intersection of the axes of the third and second molar (α) and the intersection of the axis of the mandibular plane and third molar (β). Results The data obtained underwent statistical analysis. The angle α and β showed statistically significant differences on the left side in women. In men, only the right side α angle showed significant differences. Retromolar space increased significantly on both sides for both sexes. Conclusions Third molar angulation presents different behaviors between men and women, with greater verticalization in women. Key words:Third molar, retromolar space, orthodontics. PMID:28298970

  10. Brodie bite with an extracted mandibular first molar in a young adult: a case report.

    Science.gov (United States)

    Chugh, Vinay K; Sharma, Vijay P; Tandon, Pradeep; Singh, Gyan P

    2010-05-01

    Total buccal crossbites are rare, but, when they occur, they can be extremely difficult to correct, even with surgery and orthodontics. In most patients with in-locking crossbites, the maxillary teeth erupt past their mandibular antagonists, creating severe occlusal difficulties. This article presents an adult patient with scissors-bite or partial telescoping bite bilaterally in the posterior region and an extracted mandibular first molar on the right side. She was treated with expansion of the mandibular arch, and the subsequent open bite was closed with the help of masticatory muscle exercises and high-pull headgear. The second and third molars were uprighted and moved mesially to close the extraction spaces.

  11. Sensibility and taste alterations after impacted lower third molar extractions. A prospective cohort study

    Science.gov (United States)

    Ridaura-Ruiz, Lourdes; Valmaseda-Castellón, Eduard; Berini-Aytés, Leonardo; Gay-Escoda, Cosme

    2012-01-01

    Objectives: To determine the incidence, severity and duration of lingual tactile and gustatory function impairments after lower third molar removal. Study Design: Prospective cohort study with intra-subject measures of 16 patients undergoing lower third molar extractions. Sensibility and gustatory functions were evaluated in each subject preoperatively, one week and one month after the extraction, using Semmes-Weinstein monofilaments and 5 different concentrations of NaCl, respectively. Additionally, all patients filled a questionnaire to assess subjective perceptions. Results: Although patients did not perceive any sensibility impairments, a statistically significant decrease was detected when Semmes-Weinstein monofilaments. This alteration was present at one week after the surgical procedure and fully recovered one month after the extraction. There were no variations regarding the gustatory function. Conclusions: Lower third molar removal under local anesthesia may cause light lingual sensibility impairment. Most of these alterations remain undetected to patients. These lingual nerve injuries are present one week after the extraction and recover one month after surgery. The taste seems to remain unaffected after these procedures. Key words:Lingual nerve, third molar, nerve injury, paresthesia, surgical extraction PMID:22322520

  12. Complication rates in patients using absorbable collagen sponges in third molar extraction sockets: a retrospective study.

    Science.gov (United States)

    Cho, Hoon; Jung, Hwi-Dong; Kim, Bok-Joo; Kim, Chul-Hoon; Jung, Young-Soo

    2015-02-01

    The purpose of this study is to retrospectively evaluate the postoperative complication rates for absorbable type-I collagen sponge (Ateloplug; Bioland) use in third molar extraction. From January to August 2013, 2,697 total patients undergoing third molar extraction and type-I collagen sponge application in the Department of Oral and Maxillofacial Surgery at Yonsei University Dental Hospital (1,163 patients) and Dong-A University Hospital (1,534 patients) were evaluated in a retrospective study using their operation and medical records. A total of 3,869 third molars in 2,697 patients were extracted and the extraction sockets packed with type-I collagen sponges to prevent postoperative complications. As a result, the overall complication rate was 4.52%, with 3.00% experiencing surgical site infection (SSI), 1.14% showing alveolar osteitis, and 0.39% experiencing hematoma. Of the total number of complications, SSI accounted for more than a half at 66.29%. Compared to previous studies, this study showed a relatively low incidence of complications. The use of type-I collagen sponges is recommended for the prevention of complications after third molar extraction.

  13. Human dental age estimation combining third molar(s) development and tooth morphological age predictors.

    Science.gov (United States)

    Thevissen, P W; Galiti, D; Willems, G

    2012-11-01

    In the subadult age group, third molar development, as well as age-related morphological tooth information can be observed on panoramic radiographs. The aim of present study was to combine, in subadults, panoramic radiographic data based on developmental stages of third molar(s) and morphological measurements from permanent teeth, in order to evaluate its added age-predicting performances. In the age range between 15 and 23 years, 25 gender-specific radiographs were collected within each age category of 1 year. Third molar development was classified and registered according the 10-point staging and scoring technique proposed by Gleiser and Hunt (1955), modified by Köhler (1994). The Kvaal (1995) measuring technique was applied on the indicated teeth from the individuals' left side. Linear regression models with age as response and third molar-scored stages as explanatory variables were developed, and morphological measurements from permanent teeth were added. From the models, determination coefficients (R (2)) and root-mean-square errors (RMSE) were calculated. Maximal-added age information was reported as a 6 % R² increase and a 0.10-year decrease of RMSE. Forensic dental age estimations on panoramic radiographic data in the subadult group (15-23 year) should only be based on third molar development.

  14. Prevalence of cervical enamel projection in human molars

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    Maria Regina Lima de Souza

    2014-01-01

    Full Text Available Introduction : One of the developmental anomalies of dental enamel is cervical enamel projection (CEP. The aim of this study was to assess the prevalence of CEP in maxillary and mandibular human teeth. Materials and Methods: A total of 234 human molars obtained from the tooth bank of the State University of Amazonas were used in the present study. CEP was classified as Grade 0 (absence of projection, Grade I (discrete extension of cementoenamel junction toward the furcation, Grade II (closer to furcation without invasion, and Grade III (extending to the furcation area. The evaluation was performed using macroscopic inspection of teeth faces (buccal, lingual/palatal, mesial, and distal with at least one-third of the crown on each face. Results: It was found that 17.1% of the teeth evaluated had CEP, but neither of the projections occurred on the proximal faces. Higher prevalence of CEP was found on the buccal faces and the most commonly grade of CEP found was Grade I (10.3%. Conclusions: It may be concluded that CEP occurs more frequently in mandibular molars and its diagnosis is extremely important since these projections may difficult bacterial plaque removal, leading to an inflammatory process and unnecessary endodontic treatment.

  15. Preparatory information for third molar extraction: does preference for information and behavioral involvement matter?

    NARCIS (Netherlands)

    van Wijk, A.J.; Buchanan, H.; Coulson, N.; Hoogstraten, J.

    2010-01-01

    Objective: The objectives of the present study were to: (1) evaluate the impact of high versus low information provision in terms of anxiety towards third molar extraction (TME) as well as satisfaction with information provision. (2) Investigate how preference for information and behavioral

  16. Cohort study of pain symptoms and management following impacted mandibular third molar extraction.

    Science.gov (United States)

    Motonobu, A; Hidemichi, Y; Eri, U; Takashi, T; Kenichi, K

    2017-01-01

    The aim of this study was to investigate the possibility of intravenous sedation as a useful pain-relieving option for impacted third molar extractions. A prospective cohort study was conducted among patients who underwent bilateral mandibular third molar extractions under local anaesthesia and intravenous sedation (sedation group) and patients who underwent unilateral mandibular third molar extraction under local anaesthesia alone (local anaesthesia group). The frequency of use of postoperative oral analgesia and the intensity of pain assessed using the full cup test were compared between the two groups. The maximum pain intensity (0-100) on postoperative day 1 in the sedation and local anaesthesia groups was 72.8 ± 16.98 and 84.8 ± 15.84, respectively, and the mean pain intensity was 42.2 ± 16.00 and 49.6 ± 18.94. The maximum and mean pain intensities in the sedation group were significantly milder than those in the local anaesthesia group. The number of oral analgesic doses in the sedation group was significantly smaller on the day of surgery and on postoperative day 1 than in the local anaesthesia group. The results of this study suggest that bilateral impacted mandibular third molar extractions under intravenous sedation could be a recommended treatment option. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. Long-term evaluation of Class II subdivision treatment with unilateral maxillary first molar extraction

    NARCIS (Netherlands)

    Livas, Christos; Pandis, Nikolaos; Booij, Johan Willem; Katsaros, Christos; Ren, Yijin

    2015-01-01

    Objective: To evaluate the long-term effects of asymmetrical maxillary first molar (M1) extraction in Class II subdivision treatment. Materials and Methods: Records of 20 Class II subdivision whites (7 boys, 13 girls; mean age, 13.0 years; SD, 1.7 years) consecutively treated with the Begg technique

  18. Maxillary first molar extraction in Class II malocclusion : Follow-up studies on treatment effects

    NARCIS (Netherlands)

    Livas, Christos

    2015-01-01

    This PhD research investigated treatment effects of extraction of one and two maxillary first molars in Class II subdivision and Class II/1 malocclusion cases respectively from a longer time perspective. Private practice records were scrutinized to evaluate aspects of a treatment technique combining

  19. Extraction of maxillary first permanent molars in patients with Class II Division 1 malocclusion.

    NARCIS (Netherlands)

    Stalpers, M.J.; Booij, J.W.; Bronkhorst, E.M.; Kuijpers-Jagtman, A.M.; Katsaros, C.

    2007-01-01

    INTRODUCTION: Our objectives were to assess treatment outcomes in Class II Division 1 patients who were treated orthodontically with extraction of the maxillary first permanent molars and to describe the changes in their facial profiles. METHODS: This was a prospective, longitudinal, 1-group outcome

  20. A treatment method for Class II Division 1 patients with extraction of permanent maxillary first molars.

    NARCIS (Netherlands)

    Booij, J.W.; Kuijpers-Jagtman, A.M.; Katsaros, C.

    2009-01-01

    Throughout the years, various treatment modalities have been presented for the treatment of Class II Division 1 malocclusions. The goal of this paper is to present a treatment approach that involves the extraction of the maxillary first molars followed by use of fixed appliances with low-friction br

  1. Maxillary first molar extraction in Class II malocclusion : Follow-up studies on treatment effects

    NARCIS (Netherlands)

    Livas, Christos

    2015-01-01

    This PhD research investigated treatment effects of extraction of one and two maxillary first molars in Class II subdivision and Class II/1 malocclusion cases respectively from a longer time perspective. Private practice records were scrutinized to evaluate aspects of a treatment technique combining

  2. Extraction of maxillary first permanent molars in patients with Class II Division 1 malocclusion.

    NARCIS (Netherlands)

    Stalpers, M.J.; Booij, J.W.; Bronkhorst, E.M.; Kuijpers-Jagtman, A.M.; Katsaros, C.

    2007-01-01

    INTRODUCTION: Our objectives were to assess treatment outcomes in Class II Division 1 patients who were treated orthodontically with extraction of the maxillary first permanent molars and to describe the changes in their facial profiles. METHODS: This was a prospective, longitudinal, 1-group outcome

  3. Overjet correction and space closure mechanisms for Class II treatment by extracting the maxillary first molars

    NARCIS (Netherlands)

    Booij, J.W.; Goeke, J.; Bronkhorst, E.M.; Pancherz, H.; Ruf, S.; Katsaros, C.

    2011-01-01

    OBJECTIVE: To analyze the mechanism of overjet correction and space closure when treating Class II Division 1 patients by extracting the maxillary first molars. PATIENTS AND METHODS: A total of 100 prospective, consecutively treated Class II Division 1 patients (45 female, 55 male; 10.5-17.2 years

  4. Osteomyelitis following extraction of lower third molar during pregnancy

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    Kim, Sang Bin; Huh, Kyung Hoe; Yi, Won Jin; Lee, Sam Sun [Department of Oral and Maxillofacial Radiology, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul (Korea, Republic of); Kim, Mi Ja [Department of Dentistry, Hangang Sacred Heart Hospital, Hallym University, Seoul (Korea, Republic of)

    2008-09-15

    A 27-year-old female was referred to our hospital postpartum due to rapid aggravation of facial swelling. She was diagnosed with osteomyelitis on clinical, radiological, and histopathological examinations, but the possibility of malignancy was not excluded. Clinical signs and symptoms such as facial swelling and discomfort were improved with four months of antibiotics treatment. This is a case of an osteomyelitis progressed from infected extraction socket in a woman with physiological changes of pregnancy. Decreased immunological response, increased sex hormone and calcium regulatory hormones regulate the response of the bone infection. We report this case for helping the diagnosis of unusual form of osteomyelitis in pregnancy and postpartum.

  5. A comparison of the effects of methylprednisolone and tenoxicam on pain, edema, and trismus after impacted lower third molar extraction.

    Science.gov (United States)

    Ilhan, Ozgur; Agacayak, Kamil Serkan; Gulsun, Belgin; Koparal, Mahmut; Gunes, Nedim

    2014-01-29

    The aim of the present study was to compare the effects of preemptive intravenous tenoxicam and methylprednisolone administrations on extraction of impacted third molars. This was a placebo-controlled, randomized, double-blind, clinical trial. A total of 60 adult patients ages 18-40 years with the complaints of impacted third molar teeth were included in the study. The postoperative swelling ratios (ptrismus than tenoxicam without any significant differences in edema and pain control in impacted third molar teeth extraction.

  6. Size and shape variability in human molars during odontogenesis.

    Science.gov (United States)

    Morita, W; Yano, W; Nagaoka, T; Abe, M; Nakatsukasa, M

    2014-03-01

    Under the patterning cascade model (PCM) of cusp development inspired by developmental genetic studies, it is predicted that the location and the size of later-forming cusps are more variable than those of earlier-forming ones. Here we assessed whether differences in the variability among cusps in total and each particular crown component (enamel-dentin junction [EDJ], outer enamel surface [OES], and cement-enamel junction [CEJ]) could be explained by the PCM, using human maxillary permanent first molars (UM1) and second deciduous molars (um2). Specimens were µCT-scanned, and 3D models of EDJ and OES were reconstructed. Based on these models, landmark-based 3D geometric morphometric analyses were conducted. Size variability in both tooth types was generally consistent with the above prediction, and the differences in size variation among cusps were smaller for the crown components completed in later stages of odontogenesis. With a few exceptions, however, the prediction was unsupported regarding shape variability, and UM1 and um2 showed different patterns. Our findings suggested that the pattern of size variability would be caused by temporal factors such as the order of cusp initiation and the duration from the beginning of mineralization to the completion of crown formation, whereas shape variability may be affected by both topographic and temporal factors.

  7. Unusual treatment of bimaxillary dentoalveolar protrusion via miniscrews and molar extraction.

    Science.gov (United States)

    Al-Fraidi, Ahmad; Afify, Ahmed R

    2012-04-01

    This case report describes the treatment of a Saudi female patient, aged 13 years 8 months at the start of treatment, with a Class I bimaxillary dentoalveolar protrusion and extracted maxillary first molars. Miniscrews were placed bilaterally in the interdental space between both the upper and the lower posterior teeth. The treatment plan consisted of extraction of both lower first permanent molars, distalization of upper and lower premolars using miniscrews followed by en masse retraction of the upper and lower six anterior teeth. The active treatment period was 2 years 8 months. Arch retention was done using upper wrap-around retainer and lower fixed 3-3 retainer. The use of miniscrews helped to resolve the bimaxillary protrusion regardless of extraction pattern used.

  8. A extração de segundos molares superiores para o tratamento da Classe II Extraction of upper second molars for treatment of Angle Class II malocclusion

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    Maurício Barbieri Mezomo

    2010-06-01

    Full Text Available Este trabalho tem por objetivo apresentar uma abordagem alternativa para o tratamento ortodôntico das más oclusões de Classe II. Através de uma revisão da literatura, verificou-se que a extração de segundos molares superiores demonstrou ser uma alternativa viável para o tratamento desse tipo de má oclusão. Essa opção terapêutica possibilita maior rapidez na distalização dos primeiros molares com menor necessidade de cooperação por parte do paciente. Porém, a análise do grau de formação, posição intraóssea e morfologia do terceiro molar deve ser cuidadosamente realizada para proporcionar o correto posicionamento do mesmo no lugar do segundo molar extraído. Dois casos clínicos apresentarão a sequência do diagnóstico e tratamento com essa mecânica, exibindo resultados adequados dos pontos de vista funcional e estético.The purpose of this article is to present an alternative approach to the orthodontic treatment of Angle Class II malocclusion. According to a literature review it was observed that the extraction of upper second molars has proven to be a viable alternative for the treatment of this type of malocclusion. This therapeutic option enables faster first molar retraction and requires less patient compliance. However, the level of development, intraosseous position and morphology of the third molar should be carefully evaluated to ensure its correct positioning in place of the extracted second molar. Two clinical case reports will demonstrate that the sequence of diagnosis and treatment used with this mechanics yields satisfactory functional and aesthetic results.

  9. Inferior Alveolar Nerve Injury after Mandibular Third Molar Extraction: a Literature Review

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    Rafael Sarikov

    2014-12-01

    Full Text Available Objectives: The purpose of this study was to systematically review the comprehensive overview of literature data about injury to the inferior alveolar nerve after lower third molar extraction to discover the prevalence of injury, the risk factors, recovery rates, and alternative methods of treatment. Material and Methods: Literature was selected through a search of PubMed electronic databases. Articles from January 2009 to June 2014 were searched. English language articles with a minimum of 6 months patient follow-up and injury analysis by patient’s reporting, radiographic, and neurosensory testing were selected. Results: In total, 84 literature sources were reviewed, and 14 of the most relevant articles that are suitable to the criteria were selected. Articles were analyzed on men and women. The influence of lower third molar extraction (especially impacted on the inferior alveolar nerve was clearly seen. Conclusions: The incidence of injury to the inferior alveolar nerve after lower third molar extraction was about 0.35 - 8.4%. The injury of the inferior alveolar nerve can be predicted by various radiological signs. There are few risk factors that may increase the risk of injury to the nerve such as patients over the age of 24 years old, with horizontal impactions, and extraction by trainee surgeons. Recovery is preferable and permanent injury is very rare.

  10. Sensibility and taste alterations after impacted lower third molar extractions. A prospective cohort study.

    Science.gov (United States)

    Ridaura-Ruiz, Lourdes; Figueiredo, Rui; Valmaseda-Castellón, Eduard; Berini-Aytés, Leonardo; Gay-Escoda, Cosme

    2012-09-01

    To determine the incidence, severity and duration of lingual tactile and gustatory function impairments after lower third molar removal. Prospective cohort study with intra-subject measures of 16 patients undergoing lower third molar extractions. Sensibility and gustatory functions were evaluated in each subject preoperatively, one week and one month after the extraction, using Semmes-Weinstein monofilaments and 5 different concentrations of NaCl, respectively. Additionally, all patients filled a questionnaire to assess subjective perceptions. Although patients did not perceive any sensibility impairments, a statistically significant decrease was detected when Semmes-Weinstein monofilaments. This alteration was present at one week after the surgical procedure and fully recovered one month after the extraction. There were no variations regarding the gustatory function. Lower third molar removal under local anesthesia may cause light lingual sensibility impairment. Most of these alterations remain undetected to patients. These lingual nerve injuries are present one week after the extraction and recover one month after surgery. The taste seems to remain unaffected after these procedures.

  11. An Unusual Complication After the Extraction of a Maxillary Third Molar: Extensive Subcutaneous Emphysema. A Case Report

    OpenAIRE

    Emrah Soylu

    2016-01-01

    Third molar surgery is one of the most frequently performed procedures in oral and maxillofacial surgery. Various complications including pain, bleeding, infection, edema, hematoma, perforation of the maxillary sinus, and subcutaneous emphysema (SE) can occur after third molar surgery. Cervicofacial subcutaneous emphysema (CSE) most often occurs after the extraction of third molars, especially when using high-speed air-turbine drills and air syringes, or during dental laser treatment or even ...

  12. A rare complication following maxillary third molar extraction: infratemporal fossa abscess

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    Görkem Müftüoğlu

    2012-10-01

    Full Text Available

    Infratemporal fossa abscess formation is a very rare and life threatening condition and also its differential diagnosis is a very difficult process. Infratemporal fossa abscess following the non-infected, asymptomatic, erupted maxillary third molar extraction in a young and healthy patient is an unexpected and unusual complication.

    A 25 years old, male patient with a significant infratemporal fossa abscess and his treatment protocol was presented in this case report.

  13. Effects of localmelatonin application on post-extraction sockets after third molar surgery. A pilot study

    Science.gov (United States)

    Fernández-Tresguerres, Isabel; Ortega-Aranegui, Ricardo; López-Quiles, Juan

    2014-01-01

    Objectives: The purpose of this study was to assess the anti-inflammatory, analgesic and osteogenic early effects of melatonin on post-extraction sockets ofpatients requiring third molars extraction. Study Design: A randomized, triple-blind clinical trial was made using a split-mouth design. Both lower third molars of 10 patients were extracted and 3 mg of local melatonin or placebo were applied. Concentrations of interleukin-6 and nitrotyrosine were determined on samples of the clot from the socket by independent ELISA tests. Radiographic bone density was evaluated by measuring Hounsfield Units in panoramic and cross sections obtained by digital scanner. Statistycal analysis by Kolmogorov-Smirnov test was performed for ELISA data. Bone density was analyzed by Shapiro-Wilk test. Subsequently t test was applied. P<0.05 was considered to be significant. Results: The concentration of interleukin-6 increased with the application of melatonin without statistically significance (361.32 ± 235.22 pg/ml vs 262.58 ± 233.92 pg/ml). Nitrotyrosine concentrations showed values below to the detectability pattern (<0.001 nM) in Optic Density curve. Bone density in panoramic sections at socket after melatonin application showed no significant difference (561.98 ± 105.92 HU vs 598.82 ± 209.03 HU). In cross sections, bone density in the alveolar region showed no significant difference(377.42 ± 125.67 HU vs 347.56 ± 97.02 HU). Conclusions: Within the limitations of this pilot study, no differences with the application of melatonin were found in terms of the concentration of interleukin-6 and bone density in post-extraction socket of retained mandibular third molars. Key words:Melatonin, inflammation, pain, bone density, third molar surgery. PMID:25350595

  14. Two-Step Extraction of the Lower First Molar for Class III Treatment in Adult Patient

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    Kélei Cristina de Mathias Almeida

    2016-01-01

    Full Text Available The aim of this article is to describe a case report of Class III malocclusion treatment with lower first molar extraction. The 27-year-old Caucasian male patient presented a symmetric face with a straight profile, hyperdivergent growth pattern, molar and cuspid Class III relation, and an anterior crossbite as well as a mild crowding on cuspids area, in both upper and lower arches and a tendency to posterior crossbite. The treatment was performed by the use of Haas expansion appliance followed by an initial alignment and leveling of the upper and lower arches with a fixed edgewise appliance, extraction of lower teeth aiming the correction of the incisors proclination and end the treatment with a Class I molar relationship. It resulted in a significant change in the patient’s profile, dentoalveolar Class III correction, upper arch expansion, leveling and alignment of the upper and lower arches, and improvement of tipping of the upper and lowers incisors. In cases of a dentoalveolar compensation in well positioned bone bases the treatment with fixed appliances is an alternative and extraction of lower teeth is considered.

  15. Two-Step Extraction of the Lower First Molar for Class III Treatment in Adult Patient

    Science.gov (United States)

    Paulin, Ricardo Fabris; Raveli, Taísa Barnabé; Raveli, Dirceu Barnabé

    2016-01-01

    The aim of this article is to describe a case report of Class III malocclusion treatment with lower first molar extraction. The 27-year-old Caucasian male patient presented a symmetric face with a straight profile, hyperdivergent growth pattern, molar and cuspid Class III relation, and an anterior crossbite as well as a mild crowding on cuspids area, in both upper and lower arches and a tendency to posterior crossbite. The treatment was performed by the use of Haas expansion appliance followed by an initial alignment and leveling of the upper and lower arches with a fixed edgewise appliance, extraction of lower teeth aiming the correction of the incisors proclination and end the treatment with a Class I molar relationship. It resulted in a significant change in the patient's profile, dentoalveolar Class III correction, upper arch expansion, leveling and alignment of the upper and lower arches, and improvement of tipping of the upper and lowers incisors. In cases of a dentoalveolar compensation in well positioned bone bases the treatment with fixed appliances is an alternative and extraction of lower teeth is considered. PMID:27699072

  16. Comparison of platelet rich plasma and synthetic graft material for bone regeneration after third molar extraction

    Science.gov (United States)

    Nathani, Dipesh B.; Sequeira, Joyce; Rao, B. H. Sripathi

    2015-01-01

    Aims: To compare the efficacy of Platelet rich plasma and synthetic graft material for bone regeneration after bilateral third molar extraction. Material and Methods: This study was conducted in 10 patients visiting the outpatient department of Oral & Maxillofacial Surgery, Yenepoya Dental College & Hospital. Patients requiring extraction of bilateral mandibular third molars were taken for the study. Following extraction, PRP (Platelet Rich Plasma) was placed in one extraction socket and synthetic graft material in form granules [combination of Hydroxyapatite (HA) and Bioactive glass (BG)] in another extraction socket. The patients were assessed for postoperative pain and soft tissue healing. Radiological assessment of the extraction site was done at 8, 12 and 16 weeks interval to compare the change in bone density in both the sockets. Results: Pain was less on PRP site when compared to HA site. Soft tissue evaluation done using gingival healing index given by Landry et al showed better healing on PRP site when compared to HA site. The evaluation of bone density by radiological assessment showed the grey level values calculated at 4 months at the PRP site were comparatively higher than HA site. Conclusion: The study showed that the platelet rich plasma is a better graft material than synthetic graft material in terms of soft tissue and bone healing. However a more elaborate study with a larger number of clinical cases is very much essential to be more conclusive regarding the efficacy of both the materials. PMID:26981473

  17. Efficiency of gaseous ozone in reducing the development of dry socket following surgical third molar extraction

    Science.gov (United States)

    Ahmedi, Jehona; Ahmedi, Enis; Sejfija, Osman; Agani, Zana; Hamiti, Vjosa

    2016-01-01

    Objective: The objective of this study was to assess the efficacy of ozone gas (O3) on the reduction of dry socket (DS) occurrence following surgical extraction of lower jaw third molars, influence of the indication for the extraction, and the difficulty of extraction on the incidence of DS. Materials and Methods: This study included thirty patients with bilaterally impacted third molars of mandible requiring surgical procedure for extraction. Following extraction, in the control group, saline solution was used for irrigation of extraction sockets and in the experimental group, intra-alveolar O3 was applied for 12 s (Prozone, W and H, UK, Ltd.). The surgeries were performed by the same oral surgeon. The follow-up visits were performed at 48 h and on day seven, postsurgery where the symptoms of DS were evaluated and intensity of pain has been recorded using visual analog scale 0–100. Results: In this pilot study, DS was present in 16.67% and 3.33% of cases in the control and experimental groups, respectively (P = 0.20). Conclusion: The application of O3 may reduce the incidence of DS and accelerates the recovery period after the surgery. Prophylactic use of O3 may be suggested in all patients, especially in the patients at a risk of development of DS. PMID:27403058

  18. Human third molars development: Comparison of 9 country specific populations.

    Science.gov (United States)

    Thevissen, P W; Fieuws, S; Willems, G

    2010-09-10

    The majority of age estimation models based on third molar development are constructed on samples from populations with described and outlined origin. Due to unlike research protocols these studies can rarely be compared for the evaluation of possible geographical or ethnical influences on third molar development. The aim of this study is to evaluate country specific third molar development on standardized collected and analyzed data. On panoramic radiographs selected from subjects out of 9 country specific populations (Belgium, China, Japan, Korea, Poland, Thailand, Turkey, Saudi-Arabia and South-India) the four third molar scores were registered, according to a modified Gleiser and Hunt methodology. To obtain for each subject a (factor) score which represents the degree of third molar development, a generalized linear mixed model for multivariate ordinal data was fitted on the repeated third molar scores. Differences between countries are analyzed using gender-specific regression models for these factor scores with age and country as predictors. Comparisons between countries revealed differences in speed and onset of development. However, although reaching statistical significance, differences in actual value were small and not constant over the considered age range. In all countries, at all ages, males were ahead in third molar development compared to females.

  19. Local Application of Platelet-Rich Fibrin During Lower Third Molar Extraction Improves Treatment Outcomes.

    Science.gov (United States)

    He, Yun; Chen, Junliang; Huang, Yue; Pan, Qin; Nie, Minhai

    2017-06-08

    Application of platelet-rich fibrin (PRF) during tooth extraction is able to accelerate wound healing, stimulate osseous and soft tissue regeneration, and reduce unwanted side effects. The aim of this meta-analysis was to investigate the effect of local application of PRF on controlling postoperative signs and symptoms after the extraction of an impacted lower third molar. A systematic search of PubMed, Web of Science, Embase, and the Cochrane Library was performed to identify all studies published up to October 2016 that investigated the effect of PRF on lower third molar extraction. Pain, swelling, trismus, alveolar osteitis (AO), and osteoblastic activity were extracted to evaluate the effect of PRF. After quality assessment, meta-analysis was performed with RevMan software (version 5.3; Cochrane Library Software, Oxford, UK). After the search and selection process, 10 studies were selected in this meta-analysis, including 468 cases of PRF application and 467 cases of non-PRF application. Of the studies, 9 were randomized controlled trials, including 7 split-mouth studies, and there was 1 retrospective case-control study. The results indicated that PRF significantly relieves pain (P = .01) and 3-day postoperative swelling (P = .03) and reduces the incidence of AO (P third molar extraction is a valid method for relieving pain and 3-day postoperative swelling and reducing the incidence of AO. For patients undergoing complicated surgical extraction, PRF might be a recommendation for local application into the sockets. Copyright © 2017 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  20. Efficacy of platelet-rich plasma applied to post-extraction retained lower third molar alveoli. A systematic review.

    Science.gov (United States)

    Barona-Dorado, C; González-Regueiro, I; Martín-Ares, M; Arias-Irimia, O; Martínez-González, J-M

    2014-03-01

    Dental retentions have a high prevalence among the general population and their removal can involve multiple complications. The use of platelet rich plasma has been proposed in an attempt to avoid these complications, as it contains high growth factors and stimulates diverse biological functions that facilitate the healing of soft and hard tissues. To evaluate the available scientific evidence related to the application of platelet-rich plasma in the post-extraction alveoli of a retained lower third molars. A systematic review of published literature registered in the Medline, EMBASE, Cochrane and NIH databases. The following categories were included: human randomized clinical studies. Key search words were: platelet rich plasma; platelet rich plasma and oral surgery; platelet rich in growth factors and third molar. Of 101 potentially valid articles, seven were selected, of which four were rejected as they failed to meet quality criteria. Three studies fulfilled all selection and quality criteria: Ogundipe et al.; Rutkowski et al.; Haraji et al. The studies all measured osteoblast activity by means of sintigraphy, and also registered pain, bleeding, inflammation, temperature, numbness as perceived by the patients, radiological bone density and the incidence of alveolar osteitis. Scientific evidence for the use of PRP in retained third molar surgery is poor. For this reason randomized clinical trials are needed before recommendations for the clinical application of PRP can be made.

  1. ANALYSIS OF POSTOPERATIVE PAIN AFTER EXTRACTION OF IMPACTED MANDIBULAR THIRD MOLARS AND ADMINISTRATION OF PREEMPTIVE ANALGESIA

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    Deyan Neychev

    2017-09-01

    Full Text Available patients because of the effect of pain symptoms on the healing process and quality of life. The objective of this study is to make a quantitative and qualitative assessment of the postoperative pain in patients to whom preemptive analgesia was administered. Material and Methods: This is a randomised, placebo-controlled study in 80 patients who underwent surgical removal of impacted mandibular third molars. The patients were divided into three groups – with the preemptive administration of placebo, metamizole sodium and nimesulide. The short form of the McGill Pain Questionnaire (SF-MPQ and the Visual Analogue Scale (VAS were used for assessment and analysis of postoperative pain. Results: For the first 24 hours after molar extraction the comparison of the values of the various pain components showed a superior effect of the preemptive analgesia with nimesulide for the alleviation of sensory and total pain compared to metamizole sodium and placebo. In all three groups, pain intensity was highest at postoperative hour 6. Conclusion: Preemptive use of NSAIDs in the extraction of impacted mandibular third molars reduces the perception of postoperative pain and its intensity.

  2. Efficacy of platelet-rich plasma applied to post-extraction retained lower third molar alveoli. A systematic review

    National Research Council Canada - National Science Library

    Barona-Dorado, C; González-Regueiro, I; Martín-Ares, M; Arias-Irimia, O; Martínez-González, J-M

    2014-01-01

    ... the healing of soft and hard tissues. To evaluate the available scientific evidence related to the application of platelet-rich plasma in the post-extraction alveoli of a retained lower third molars...

  3. Comparison of clinical parameters and environmental noise levels between regular surgery and piezosurgery for extraction of impacted third molars

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    Hao-Hueng Chang

    2015-10-01

    Conclusion: The piezosurgery device produced noise levels similar to or lower than those of the high-speed drilling device. However, piezosurgery provides advantages of increased patient comfort during extraction of impacted third molars.

  4. Efficacy of nimesulide versus meloxicam in the control of pain, swelling and trismus following extraction of impacted lower third molar.

    Science.gov (United States)

    De Menezes, S A F; Cury, P R

    2010-06-01

    This study compared the efficacy of nimesulide and meloxicam in the control of pain, swelling and trismus, following the extraction of impacted inferior third molars. Twenty patients with two impacted inferior third molars, in similar positions, were selected. The patients were designated randomly to the meloxicam group (MEL: 7.5mg twice a day for 5 days) or the nimesulide group (NIM: 100mg for 5 days). Following the extractions, swelling was more pronounced in the MEL group than in the NIM group (P0.05). At the 72-h evaluation, reduction was significantly larger in mouth opening in the MEL group compared with the NIM group (Pimpacted lower third molars.

  5. Analysis of potential dynamic concealed factors in the difficulty of lower third molar extraction

    Science.gov (United States)

    Singh, Pradeep; Ajmera, Deepal-Haresh; Xiao, Shui-Sheng; Liu, Xiong; Peng, Bin

    2016-01-01

    Background The purpose of this study was to identify potential concealed variables associated with the difficulty of lower third molar (M3) extractions. Material and Methods To address the research purpose, we implemented a prospective study and enrolled a sample of subjects presenting for M3 removal. Predictor variables were categorized into Group-I and Group-II, based on predetermined criteria. The primary outcome variable was the difficulty of extraction, measured as extraction time. Appropriate univariate and multivariate statistics were computed using ordinal logistic regression. Results The sample comprised of 1235 subjects with a mean age of 29.49 +/- 8.92 years in Group-I and 26.20 +/- 11.55 years in Group-II subjects. The mean operating time per M3 extraction was 21.24 +/- 12.80 and 20.24 +/- 12.50 minutes for Group-I and Group-II subjects respectively. Three linear parameters including B-M2 height (distance between imaginary point B on the inferior border of mandibular body, and M2), lingual cortical thickness, bone density and one angular parameter including Rc-Cs angle (angle between ramus curvature and curve of spee), in addition to patient’s age, profile type, facial type, cant of occlusal plane, and decreased overbite, were found to be statistically associated (P < or = 0.05) with extraction difficulty under regression models. Conclusions In conclusion, our study indicates that the difficulty of lower M3 extractions is possibly governed by morphological and biomechanical factors with substantial influence of myofunctional factors. Practical Implications: Preoperative evaluation of dynamic concealed factors may not only help in envisaging the difficulty and planning of surgical approach but might also help in better time management in clinical practice. Key words:Third molar, impacted, extraction, mandibular, facial type. PMID:27694781

  6. An Unusual Complication After the Extraction of a Maxillary Third Molar: Extensive Subcutaneous Emphysema. A Case Report

    Directory of Open Access Journals (Sweden)

    Emrah Soylu

    2016-09-01

    Full Text Available Third molar surgery is one of the most frequently performed procedures in oral and maxillofacial surgery. Various complications including pain, bleeding, infection, edema, hematoma, perforation of the maxillary sinus, and subcutaneous emphysema (SE can occur after third molar surgery. Cervicofacial subcutaneous emphysema (CSE most often occurs after the extraction of third molars, especially when using high-speed air-turbine drills and air syringes, or during dental laser treatment or even after endodontic treatment. This report presents the diagnosis and treatment protocol of a CSE in a 42-year-old healthy male patient that occurred after extraction of a totally erupted upper third molar with just a straight elevator and extraction forceps.

  7. Is there justification for prophylactic extraction of third molars? A systematic review

    Directory of Open Access Journals (Sweden)

    Moacir Guilherme da Costa

    2013-04-01

    Full Text Available The present systematic review was performed to investigate if there is evidence justifying the prophylactic extraction of third molars, one of the most frequent procedures in oral surgery. A series of searches was carried out for randomized, clinical trials and systematic reviews in seven databases (MEDLINE, BBO, LILACS, Web of Science, EMBASE, BIREME and Cochrane Library, with no restrictions regarding year or language. A supplemental manual search of the references of retrieved articles was also performed. The search strategy resulted in 260 papers. Both the data extracted and the quality of each paper were evaluated independently by two reviewers. After selection based on the preestablished eligibility criteria, four papers qualified for the final analysis. A medium degree of quality and methodological consistency was found in three studies, and low quality was found in one study. No studies showed a high degree of consistency. The most significant flaw was an inadequate sample size. The results of the present review indicate a lack of scientific evidence to justify the indication of the prophylactic extraction of third molars.

  8. The anxiolytic effect of midazolam in third molar extraction: a systematic review.

    Directory of Open Access Journals (Sweden)

    Qi Chen

    Full Text Available To assess the efficacy of midazolam for anxiety control in third molar extraction surgery.Electronic retrievals were conducted in Medline (via PubMed, 1950-2013.12, the Cochrane Central Register of Controlled Trials (CENTRAL (The Cochrane Library 2013, Issue 3, Embase (via OVID 1974-2013.12, and the System for Information on Grey Literature in Europe (SIGLE. The bibliographies of relevant clinical trials were also checked. Randomized controlled trials satisfying the inclusion criteria were evaluated, with data extraction done independently by two well-trained investigators. Disagreements were resolved by discussion or by consultation with a third member of the review team.Ten studies were included, but meta-analysis could not be conducted because of the significant differences among articles. All but one article demonstrated that midazolam could relieve anxiety. One article demonstrated that propofol offered superior anxiolysis, with more rapid recovery than with midazolam. Compared with lorazepam and diazepam, midazolam did not distinctly dominate in its sedative effect, but was safer. Two articles used midazolam in multidrug intravenous sedation and proved it to be more effective than midazolam alone.It was found, by comparison and analysis, that midazolam might be effective for use for anxiety control during third molar extraction and can be safely administered by a dedicated staff member. It can also be used with other drugs to obtain better sedative effects, but the patient's respiratory function must be monitored closely, because multidrug sedation is also more risky.

  9. The Anxiolytic Effect of Midazolam in Third Molar Extraction: A Systematic Review

    Science.gov (United States)

    Chen, Qi; Wang, Lufei; Ge, Lina; Gao, Yuan; Wang, Hang

    2015-01-01

    Purpose To assess the efficacy of midazolam for anxiety control in third molar extraction surgery. Methods Electronic retrievals were conducted in Medline (via PubMed, 1950-2013.12), the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2013, Issue 3), Embase (via OVID 1974-2013.12), and the System for Information on Grey Literature in Europe (SIGLE). The bibliographies of relevant clinical trials were also checked. Randomized controlled trials satisfying the inclusion criteria were evaluated, with data extraction done independently by two well-trained investigators. Disagreements were resolved by discussion or by consultation with a third member of the review team. Results Ten studies were included, but meta-analysis could not be conducted because of the significant differences among articles. All but one article demonstrated that midazolam could relieve anxiety. One article demonstrated that propofol offered superior anxiolysis, with more rapid recovery than with midazolam. Compared with lorazepam and diazepam, midazolam did not distinctly dominate in its sedative effect, but was safer. Two articles used midazolam in multidrug intravenous sedation and proved it to be more effective than midazolam alone. Conclusion It was found, by comparison and analysis, that midazolam might be effective for use for anxiety control during third molar extraction and can be safely administered by a dedicated staff member. It can also be used with other drugs to obtain better sedative effects, but the patient’s respiratory function must be monitored closely, because multidrug sedation is also more risky. PMID:25849859

  10. Antibiotics in third molar extraction; are they really necessary: A non-inferiority randomized controlled trial

    Science.gov (United States)

    Arora, Ankit; Roychoudhury, Ajoy; Bhutia, Ongkila; Pandey, Sandeep; Singh, Surender; Das, Bimal K.

    2014-01-01

    Introduction: Antibiotic resistance is now a serious problem, although it was not so only a few years ago. The need of the hour is to give clear evidence of the efficacy of antibiotic use, or lack thereof, to the surgeon for a procedure as common as mandibular third molar surgery. Aim: This study aimed to evaluate whether postoperative combined amoxicillin and clavulanic acid in mandibular third molar extraction is effective in preventing inflammatory complications. Study and Design: The study was structured as a prospective randomized double-blind placebo-controlled clinical trial. Materials and Methods: A study was designed wherein the 96 units (two bilaterally similar impacted mandibular third molars per head in 48 patients) were randomly assigned to two treatment groups (Group I and Group II). Each patient served as his/her own control. Each patient received 625 mg of combined amoxicillin and clavulanic acid 1 h before surgery. In the case of third molars belonging to Group I, 625 mg of combined amoxicillin and clavulanic acid TDS was continued for 3 days; in Group II, placebo in similar-looking packs was continued for 3 days. The patients were evaluated on the third and seventh postoperative days for signs of clinical infection and for microbial load evaluation. Statistical Analysis: The data between the two groups were statistically analyzed by the two-tailed Fisher's exact test, with a 95% confidence interval. Results: The difference was not statistically significant between the test group and the control group with regard to erythema, dehiscence, swelling, pain, trismus, and infection based on microbial load. The data were statistically significant for alveolar osteitis, with the occurrence of alveolar osteitis (14.58%) in the placebo group. Conclusion: Postoperative antibiotics are recommended only for patients undergoing contaminated, long-duration surgery. PMID:25937728

  11. Maxillary sinus floor extension and posterior tooth inclination in adolescent patients with Class II Division 1 malocclusion treated with maxillary first molar extractions

    NARCIS (Netherlands)

    Livas, Christos; Halazonetis, Demetrios J.; Booij, Johan Willem; Pandis, Nikolaos; Tu, Yu-Kang; Katsaros, Christos

    2013-01-01

    Introduction: Our objective was to investigate potential associations between maxillary sinus floor extension and inclination of maxillary second premolars and second molars in patients with Class II Division 1 malocclusion whose orthodontic treatment included maxillary first molar extractions. Meth

  12. Maxillary sinus floor extension and posterior tooth inclination in adolescent patients with Class II Division 1 malocclusion treated with maxillary first molar extractions

    NARCIS (Netherlands)

    Livas, C.; Halazonetis, D.J.; Booij, J.W.; Pandis, N.; Tu, Y.K.; Katsaros, C.

    2013-01-01

    INTRODUCTION: Our objective was to investigate potential associations between maxillary sinus floor extension and inclination of maxillary second premolars and second molars in patients with Class II Division 1 malocclusion whose orthodontic treatment included maxillary first molar extractions. METH

  13. Maxillary sinus floor extension and posterior tooth inclination in adolescent patients with Class II Division 1 malocclusion treated with maxillary first molar extractions

    NARCIS (Netherlands)

    Livas, Christos; Halazonetis, Demetrios J.; Booij, Johan Willem; Pandis, Nikolaos; Tu, Yu-Kang; Katsaros, Christos

    Introduction: Our objective was to investigate potential associations between maxillary sinus floor extension and inclination of maxillary second premolars and second molars in patients with Class II Division 1 malocclusion whose orthodontic treatment included maxillary first molar extractions.

  14. Maxillary sinus floor extension and posterior tooth inclination in adolescent patients with Class II Division 1 malocclusion treated with maxillary first molar extractions

    NARCIS (Netherlands)

    Livas, C.; Halazonetis, D.J.; Booij, J.W.; Pandis, N.; Tu, Y.K.; Katsaros, C.

    2013-01-01

    INTRODUCTION: Our objective was to investigate potential associations between maxillary sinus floor extension and inclination of maxillary second premolars and second molars in patients with Class II Division 1 malocclusion whose orthodontic treatment included maxillary first molar extractions.

  15. Patients’ satisfaction and prevalence of complications on surgical extraction of third molar

    Directory of Open Access Journals (Sweden)

    Lee CTY

    2015-02-01

    Full Text Available Crystal TY Lee, Shinan Zhang, Yiu Yan Leung, Samantha KY Li, Cissy C Tsang, Chun-Hung Chu Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, People’s Republic of China Objectives: To study patients’ satisfaction and prevalence of complications in surgical extraction of impacted third molar by senior dentists and recently graduated dentists in a university dental clinic. Method: Patients who had impacted third molar extraction in a university dental clinic by two associate dentists who had <2 years of experience and two senior dentists who had >15 years of experience were evaluated in this study. Patients’ age, sex, history of pericoronitis, tooth extracted, and radiographic assessment of the impacted tooth were recorded. Immediately after suture removal, the patients were invited to indicate their satisfaction on a Likert scale of 1–5. Results: A total of 546 patients received extraction, and 251 patients were operated by associate dentists. Patient satisfaction was higher among those who had noncomplicated surgery (P=0.007, short treatment time (P<0.001, and had no postsurgical emergency appointments (P<0.001. The prevalence of seeking postsurgical emergency appointments was 9.2%. The reasons were severe pain (4.8%, swelling (2.6%, bleeding (2.4%, alveolar osteitis (0.9%, paresthesia (0.9, and trismus (0.5%. The prevalence of postsurgical complication(s in associate dentists and senior dentists was 11.6% and 7.1%, respectively (P=0.050. The mean satisfaction scores for associate dentists and senior dentists were 4.17 and 3.95, respectively (P=0.002. Conclusion: Although a higher rate of postsurgical complications was observed among the patients treated by the recently graduated dentists, their patients’ satisfaction scores were higher than that of the senior dentists. Around 9% of patients attended postsurgical emergency appointments, and their common reason was severe pain. Keywords: dental satisfaction, dentistry

  16. Patients’ satisfaction and prevalence of complications on surgical extraction of third molar

    Science.gov (United States)

    Lee, Crystal TY; Zhang, Shinan; Leung, Yiu Yan; Li, Samantha KY; Tsang, Cissy C; Chu, Chun-Hung

    2015-01-01

    Objectives To study patients’ satisfaction and prevalence of complications in surgical extraction of impacted third molar by senior dentists and recently graduated dentists in a university dental clinic. Method Patients who had impacted third molar extraction in a university dental clinic by two associate dentists who had 15 years of experience were evaluated in this study. Patients’ age, sex, history of pericoronitis, tooth extracted, and radiographic assessment of the impacted tooth were recorded. Immediately after suture removal, the patients were invited to indicate their satisfaction on a Likert scale of 1–5. Results A total of 546 patients received extraction, and 251 patients were operated by associate dentists. Patient satisfaction was higher among those who had noncomplicated surgery (P=0.007), short treatment time (Pprevalence of seeking postsurgical emergency appointments was 9.2%. The reasons were severe pain (4.8%), swelling (2.6%), bleeding (2.4%), alveolar osteitis (0.9%), paresthesia (0.9), and trismus (0.5%). The prevalence of postsurgical complication(s) in associate dentists and senior dentists was 11.6% and 7.1%, respectively (P=0.050). The mean satisfaction scores for associate dentists and senior dentists were 4.17 and 3.95, respectively (P=0.002). Conclusion Although a higher rate of postsurgical complications was observed among the patients treated by the recently graduated dentists, their patients’ satisfaction scores were higher than that of the senior dentists. Around 9% of patients attended postsurgical emergency appointments, and their common reason was severe pain. PMID:25709411

  17. Patients' satisfaction and prevalence of complications on surgical extraction of third molar.

    Science.gov (United States)

    Lee, Crystal Ty; Zhang, Shinan; Leung, Yiu Yan; Li, Samantha Ky; Tsang, Cissy C; Chu, Chun-Hung

    2015-01-01

    To study patients' satisfaction and prevalence of complications in surgical extraction of impacted third molar by senior dentists and recently graduated dentists in a university dental clinic. Patients who had impacted third molar extraction in a university dental clinic by two associate dentists who had 15 years of experience were evaluated in this study. Patients' age, sex, history of pericoronitis, tooth extracted, and radiographic assessment of the impacted tooth were recorded. Immediately after suture removal, the patients were invited to indicate their satisfaction on a Likert scale of 1-5. A total of 546 patients received extraction, and 251 patients were operated by associate dentists. Patient satisfaction was higher among those who had noncomplicated surgery (P=0.007), short treatment time (Pprevalence of seeking postsurgical emergency appointments was 9.2%. The reasons were severe pain (4.8%), swelling (2.6%), bleeding (2.4%), alveolar osteitis (0.9%), paresthesia (0.9), and trismus (0.5%). The prevalence of postsurgical complication(s) in associate dentists and senior dentists was 11.6% and 7.1%, respectively (P=0.050). The mean satisfaction scores for associate dentists and senior dentists were 4.17 and 3.95, respectively (P=0.002). Although a higher rate of postsurgical complications was observed among the patients treated by the recently graduated dentists, their patients' satisfaction scores were higher than that of the senior dentists. Around 9% of patients attended postsurgical emergency appointments, and their common reason was severe pain.

  18. Class II treatment by extraction of maxillary first molars or Herbst appliance: dentoskeletal and soft tissue effects in comparison

    NARCIS (Netherlands)

    Booij, J.W.; Goeke, J.; Bronkhorst, E.M.; Katsaros, C.; Ruf, S.

    2013-01-01

    AIM: To compare dentoskeletal and soft tissue treatment effects of two alternative Class II division 1 treatment modalities (maxillary first permanent molar extraction versus Herbst appliance). METHODS: One-hundred-fifty-four Class II division 1 patients that had either been treated with extractions

  19. Class II treatment by extraction of maxillary first molars or Herbst appliance: dentoskeletal and soft tissue effects in comparison

    NARCIS (Netherlands)

    Booij, J.W.; Goeke, J.; Bronkhorst, E.M.; Katsaros, C.; Ruf, S.

    2013-01-01

    AIM: To compare dentoskeletal and soft tissue treatment effects of two alternative Class II division 1 treatment modalities (maxillary first permanent molar extraction versus Herbst appliance). METHODS: One-hundred-fifty-four Class II division 1 patients that had either been treated with extractions

  20. Morphological and anthropological aspects of human triangular deciduous lower first molar teeth.

    Science.gov (United States)

    Kitagawa, Y; Manabe, Y; Oyamada, J; Rokutanda, A

    1996-04-01

    The crown and root morphology, and bilateral occurrence of human deciduous lower first molars that exhibited a triangular occlusal outline, taken from excavated samples of Japanese, Jomonese and Iraqi origin, were investigated. The crowns of triangular teeth had smaller mesiodistal and larger buccolingual diameters than normally shaped deciduous lower first molars. An elongated buccolingual diameter was derived from the buccal projection of the distobuccal cusp and lingual projection of the portion between the metaconid and distolingual cusp. In this analysis, all triangular deciduous lower first molars in which root morphology could be observed were accompanied by additional distolingual roots. Correlation between the right- and left-hand sides of this trait was high.

  1. Human age estimation combining third molar and skeletal development.

    Science.gov (United States)

    Thevissen, P W; Kaur, J; Willems, G

    2012-03-01

    The wide prediction intervals obtained with age estimation methods based on third molar development could be reduced by combining these dental observations with age-related skeletal information. Therefore, on cephalometric radiographs, the most accurate age-estimating skeletal variable and related registration method were searched and added to a regression model, with age as response and third molar stages as explanatory variable. In a pilot set up on a dataset of 496 (283 M; 213 F) cephalometric radiographs, the techniques of Baccetti et al. (2005) (BA), Seedat et al. (2005) (SE), Caldas et al. (2007) and Rai et al. (2008) (RA) were verified. In the main study, data from 460 (208 F, 224 M) individuals in an age range between 3 and 26 years, for which at the same day an orthopantogram and a cephalogram were taken, were collected. On the orthopantomograms, the left third molar development was registered using the scoring system described by Gleiser and Hunt (1955) and modified by Köhler (1994) (GH). On the cephalograms, cervical vertebrae development was registered according to the BA and SE techniques. A regression model, with age as response and the GH scores as explanatory variable, was fitted to the data. Next, information of BA, SE and BA + SE was, respectively, added to this model. From all obtained models, the determination coefficients and the root mean squared errors were calculated. Inclusion of information from cephalograms based on the BA, as well as the SE, technique improved the amount of explained variance in age acquired from panoramic radiographs using the GH technique with 48%. Inclusion of cephalometric BA + SE information marginally improved the previous result (+1%). The RMSE decreased with 1.93, 1.85 and 2.03 years by adding, respectively, BA, SE and BA + SE information to the GH model. The SE technique allows clinically the fastest and easiest registration of the degree of development of the cervical vertebrae. Therefore, the choice of

  2. Comparison of oral versus sublingual piroxicam during postoperative pain management after lower third molar extraction.

    Science.gov (United States)

    Trindade, P A K; Giglio, F P M; Colombini-Ishikiriama, B L; Calvo, A M; Modena, K C S; Ribeiro, D A; Dionísio, T J; Brozoski, D T; Lauris, J R P; Faria, F A C; Santos, C F

    2011-03-01

    In this study, 53 patients received piroxicam, administered orally or sublingually, after undergoing removal of symmetrically positioned lower third molars, during two separate appointments. This study used a randomized, blind, cross-over protocol. Objective and subjective parameters were recorded for comparison of postoperative results for 7 days after surgery. Patients treated with oral or sublingual piroxicam reported low postoperative pain scores. The patients who received piroxicam orally took a similar average amount of analgesic rescue medication compared with patients who received piroxicam sublingually (p>0.05). Patients exhibited similar values for mouth opening measured just before surgery and immediately following suture removal 7 days later (p>0.05), and showed no significant differences between routes of piroxicam administration for swelling control during the second or seventh postoperative days (p>0.05). In summary, pain, trismus and swelling after lower third molar extraction, independent of surgical difficulty, could be controlled by piroxicam 20mg administered orally or sublingually and no significant differences were observed between the route of delivery used in this study.

  3. Volume analysis of heat-induced cracks in human molars: A preliminary study

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    Michael A. Sandholzer

    2014-01-01

    Full Text Available Context: Only a few methods have been published dealing with the visualization of heat-induced cracks inside bones and teeth. Aims : As a novel approach this study used nondestructive X-ray microtomography (micro-CT for volume analysis of heat-induced cracks to observe the reaction of human molars to various levels of thermal stress. Materials and Methods: Eighteen clinically extracted third molars were rehydrated and burned under controlled temperatures (400, 650, and 800°C using an electric furnace adjusted with a 25°C increase/min. The subsequent high-resolution scans (voxel-size 17.7 μm were made with a compact micro-CT scanner (SkyScan 1174. In total, 14 scans were automatically segmented with Definiens XD Developer 1.2 and three-dimensional (3D models were computed with Visage Imaging Amira 5.2.2. The results of the automated segmentation were analyzed with an analysis of variance (ANOVA and uncorrected post hoc least significant difference (LSD tests using Statistical Package for Social Sciences (SPSS 17. A probability level of P < 0.05 was used as an index of statistical significance. Results: A temperature-dependent increase of heat-induced cracks was observed between the three temperature groups (P < 0.05, ANOVA post hoc LSD. In addition, the distributions and shape of the heat-induced changes could be classified using the computed 3D models. Conclusion: The macroscopic heat-induced changes observed in this preliminary study correspond with previous observations of unrestored human teeth, yet the current observations also take into account the entire microscopic 3D expansions of heat-induced cracks within the dental hard tissues. Using the same experimental conditions proposed in the literature, this study confirms previous results, adds new observations, and offers new perspectives in the investigation of forensic evidence.

  4. Manual reduction of articular disc after traumatic extraction of mandibular third molar: a case report

    Directory of Open Access Journals (Sweden)

    Rubens Camino Junior

    2015-10-01

    Full Text Available Introduction: Disc displacement without reduction with limited opening is an intracapsular biomechanical disorder involving the condyle-disc complex. With the mouth closed, the disc is in an anterior position in relation to the condylar head and does not reduce with mouth opening. This disorder is associated with persistent limited mandibular opening.Case report:The patient presented severe limitation to fully open the mouth, interfering in her ability to eat. Clinical examination also revealed maximum assisted jaw opening (passive stretch with less than 40 mm of maximum interincisal opening. Magnetic resonance imaging was the method of choice to identify the temporomandibular disorders.Conclusion: By means of reporting this rare case of anterior disc displacement without reduction with limited opening, after traumatic extraction of a mandibular third molar, in which manual reduction of temporomandibular joint articular disc was performed, it was possible to prove that this technique is effective in the prompt restoration of mandibular movements.

  5. Prevention of trismus with different pharmacological therapies after surgical extraction of impacted mandibular third molar.

    Science.gov (United States)

    Selimović, Edin; Ibrahimagić-Šeper, Lejla; Šišić, Ibrahim; Sivić, Suad; Huseinagić, Senad

    2017-02-01

    Aim To assess prevention and reduction of trismus after surgically extracted impacted mandibular third molars with individual and combined therapy with corticosteroids and anti-inflammatory analgesics. Methods The research included 60 randomly selected patients (3 groups) attended to the Dental Oral Surgery of the Public Institution Healthcare Center Zenica during the period January-December 2008. Patients of both genders, 18-45 years of age, were presented without pain and other inflammatory symptoms at the time of surgery. According to a scheme established in the research protocol, two medications were administered orally: methylprednisolone(corticosteroid) 32 mg and meloxicam (non-steroidal anti-inflammatory analgesic, NSAID) 15 mg as a single drug, or a combination of both drugs. The level of trismus is assessed on the basis of differences of preoperative and postoperative values of interincisal spaces when fully opening the mouth on the second and the seventh post-operative day. The differences between groups of patients were evaluated by means of Tukey's HSD test. Results On the second and on the seventh post-operative day significantly better results were registered in the group that received only corticosteroids and in the group that received both, corticosteroids and NSAIDs compared to the group that received only NSAIDs. A tendency of trismus reduction was present in all patient groups for the second and seventh day after surgery. Conclusion Prevention and control of postoperative trismus after surgical extraction of impacted mandibular third molars with combined therapy is effective and superior comparing to individual therapy with meloxicam-or methylprednisolone alone. Copyright© by the Medical Assotiation of Zenica-Doboj Canton.

  6. Moderate evidence to recommend submucosal injection of dexamethasone in reducing post-operative oedema and pain after third molar extraction.

    Science.gov (United States)

    Freda, Nicolas M; Keenan, Analia Veitz

    2016-06-01

    Data sourcesThe electronic databases searched included: PubMed/MEDLINE, Cochrane Central Register of Controlled Trials (Central) and Web of Science until June 2015. There was no restriction to language and the reference lists from relevant studies were searched for further articles.Study selectionRandomised and prospective controlled trials that compared the effect of submucosal injection of dexamethasone with that of placebo after impacted third molar surgery in humans. Studies involving volunteers with decompensated metabolic disease were excluded.Data extraction and synthesisStudy selection, data extraction and quality assessment (risk of bias) were assessed by two reviewers. All disagreements were resolved through discussion. A meta-analysis was performed for all continuous variables (oedema, pain and trismus) when at least two of the studies analysed the same data type.ResultsEight studies involving a total of 476 patients of which six were included in the meta-analysis. All of the surgical procedures were performed on the lower molars, submucosal injections of dexamethasone were used in concentrations of 4 mg, 8 mg, or 10 mg, and saline was used as a control. Antibiotic medications were administered prophylactically before surgery or by continuous use after the procedure. Seven of the eight studies identified the impactions according to the Pell and Gregory Classification. Oedema was measured using facial contours of pre-established reference points. The meta-analysis presented a mean difference (MD) of -2.20 (95% CI -2.70 to -1.70), with a statistically significant difference favouring dexamethasone (Pincluding a VAS were used for meta-analysis. Pain presented with a MD of -1.79 (95% CI -3.28 to -0.30) and showed a statistically significant difference favouring dexamethasone.ConclusionsThe review found moderate quality evidence that submucosal injections of dexamethasone reduced post-operative oedema and pain compared to a placebo following impacted third

  7. Success Rate of Formocresol Pulpotomy versus Mineral Trioxide Aggregate in Human Primary Molar Tooth

    Directory of Open Access Journals (Sweden)

    S E Jabbarifar

    2004-12-01

    Full Text Available Background: In spite of long time and broad use of formaldehyde derivates (Fixation agent in primary tooth pulp treatment, There is some concerns about these derivates such as variability, inconsistency success rate, mutagenicity, cytotoxicity, alergenicity, and some other potential health hazards of them. Therefore other alternative pulpotomy procedures like Bioactive glass (BAG, Glutaraldehyde (2%, Hydroxyappetite (HA, Bone dried freezed (BDF, ferric sulfate (15%, laser, Electrosurgery (ES, Bone Morphogenic proteins (BMP, recombinant protein-1 (RP1, and Mineral Trioxide Aggregate (MTA have been compared. The purpose of this clinical trial is to assess radiographic and clinical success rate of Formocresol (FC pulpotomy in compare with MTA in human primary molar teeth. Methods: 64 molars were pulpotomized equally and randomly with mineral trioxide Aggregate and Formocresol. Prior to trial, we defined a case as failure, when one or more of the events such as external root resorption, internal root resorption, periapical and furca lucency, pain, swelling, mobility, dental abscess, or early extraction appeared. Every treated tooth was defined as successful, if any noted evident was not shown. Results: Totally, 60 teeth treatment (92.2 percent were successful and 7.8 percent were failed. Failure and success rates for MTA group were 6.3 and 93.7 percent, respectively. Failure and success rates in FC group were 8.4 and 90.2 percent respectively. The difference between MTA and FC treatment methods was not significant (Fisher Exact test. Conclusion: Findings of this study show that mineral trioxide aggregate can be an alternative procedure for FC pulpotomy of primary tooth. Keywords: Mineral trioxide aggregate, formocresol, pulpotomy, success and failure rate.

  8. Surgical extraction of deeply horizontally impacted mandibular second and third molars.

    Science.gov (United States)

    Boffano, Paolo; Gallesio, Cesare; Bianchi, Francesca; Roccia, Fabio

    2010-03-01

    The presence of deeply horizontally impacted lower second and third molars is unusual. The arrested eruption of the lower second and third molars can determine disturbances of mastication and aesthetics. Moreover, an increased risk of caries in the distal side of the first lower molar is possible. Different therapeutic approaches could be proposed for second and third molar impaction and malposition. In this article, we report the management and the outcome of the surgical treatment of a patient with impacted mandibular second and third molars.

  9. Amoxicillin and clavulanic acid vs ceftazidime in the surgical extraction of impacted third molar: a comparative study.

    Science.gov (United States)

    Sisalli, U; Lalli, C; Cerone, L; Maida, S; Manzoli, L; Serra, E; Dolci, M

    2012-01-01

    The objective of this work is to compare the effectiveness and the side effects of two different drugs, amoxicillin and clavulanic acid vs ceftazidime, used as antibiotic prophylaxis in the surgical extraction of third molars and to demonstrate that the use of second choice antibiotic has no significant advantages in comparison with a first choice antibiotic. One hundred and seven patients with impacted third molar were selected and divided into two groups: amoxicillin and clavulanic acid were administered to group 1 and ceftazidime to group 2 for five days after surgery and we observed the postoperative period. The statistical analysis showed no differences between the two groups which lead to the conclusion that there is no indication to routinely administrate intramuscular second-choice antibiotic prophylatic therapy (ceftazidime) in case of surgical extraction of the third molar.

  10. [Radiographic and histological study of a case of apexification in a human molar].

    Science.gov (United States)

    Sahli, C C

    1989-01-01

    A case of apexification in a lower right second molar is described. Radiographs demonstrate apical closure with a different morphological pattern from that of the lower left second molar. Following extraction, after 15 months, serial histologic sections show calcified tissue obturating the apical foramen, well adapted to the initial dentin and cementum walls. Inside some small areas containing connective tissue with capillaries can be observed. The histologic and radiographic observations indicate that apical closure occurs as a result of differentiation of periodontal apical cells.

  11. A Review of In-Office Dynamic Image Navigation for Extraction of Complex Mandibular Third Molars.

    Science.gov (United States)

    Emery, Robert W; Korj, Oxana; Agarwal, Ravi

    2017-08-01

    We performed a retrospective review of in-office removal of complex mandibular third molars with a dynamic image navigation system (DINS). A retrospective review was conducted of cases completed from 2010 to 2014 by a single oral and maxillofacial surgeon. The average age of the patients was 47 years (range, 27 to 72 years). Extraction complexity was classified with Juodzbalys and Daugela's classification system. The included study cases had complexity scores of 9 or greater. Each patient received custom intraoral splints to secure the tracking array and underwent cone beam computed tomography image acquisition. All surgical procedures were performed with a precalibrated tracking straight handpiece under dynamic navigation. All 25 cases were treated successfully with the use of the DINS. Twelve of these cases were associated with pathologic lesions. Three patients were noted to have inferior alveolar nerve paresthesia. One patient sustained a pathologic fracture at week 2. Postoperative infections were noted in 7 cases, 2 of which had a pre-existing infection. One patient reported temporary limitation of mouth opening. A coronectomy was performed in 1 case. We present results using a new technology, the DINS, for removal of complex mandibular third molars. Potential advantages are 1) improved visualization and localization of anatomic structures such as the inferior alveolar nerve, lingual cortical plate, and adjacent roots; 2) improved control during osteotomy; 3) decreased surgical access requirements and reduction in overall bone removal; 4) ability to perform complex procedures successfully in an in-office setting; 5) decreased surgical time resulting from improved visualization; and 6) potential use as a teaching tool. Possible limitations of the use of an in-office DINS include increased cost, increased time attributed to presurgical planning, initial learning curve, and optical array interference by the surgeon or assistants during surgery. Copyright

  12. New methodology to reconstruct in 2-D the cuspal enamel of modern human lower molars.

    Science.gov (United States)

    Modesto-Mata, Mario; García-Campos, Cecilia; Martín-Francés, Laura; Martínez de Pinillos, Marina; García-González, Rebeca; Quintino, Yuliet; Canals, Antoni; Lozano, Marina; Dean, M Christopher; Martinón-Torres, María; Bermúdez de Castro, José María

    2017-08-01

    In the last years different methodologies have been developed to reconstruct worn teeth. In this article, we propose a new 2-D methodology to reconstruct the worn enamel of lower molars. Our main goals are to reconstruct molars with a high level of accuracy when measuring relevant histological variables and to validate the methodology calculating the errors associated with the measurements. This methodology is based on polynomial regression equations, and has been validated using two different dental variables: cuspal enamel thickness and crown height of the protoconid. In order to perform the validation process, simulated worn modern human molars were employed. The associated errors of the measurements were also estimated applying methodologies previously proposed by other authors. The mean percentage error estimated in reconstructed molars for these two variables in comparison with their own real values is -2.17% for the cuspal enamel thickness of the protoconid and -3.18% for the crown height of the protoconid. This error significantly improves the results of other methodologies, both in the interobserver error and in the accuracy of the measurements. The new methodology based on polynomial regressions can be confidently applied to the reconstruction of cuspal enamel of lower molars, as it improves the accuracy of the measurements and reduces the interobserver error. The present study shows that it is important to validate all methodologies in order to know the associated errors. This new methodology can be easily exportable to other modern human populations, the human fossil record and forensic sciences. © 2017 Wiley Periodicals, Inc.

  13. Efficacy of Platelet-Rich Fibrin After Mandibular Third Molar Extraction: A Systematic Review and Meta-Analysis.

    Science.gov (United States)

    Al-Hamed, Faez Saleh; Tawfik, Mohamed Abdel-Monem; Abdelfadil, Ehab; Al-Saleh, Mohammed A Q

    2017-06-01

    To assess the effect of platelet-rich fibrin (PRF) on the healing process of the alveolar socket after surgical extraction of the mandibular third molars. PubMed, the Cochrane Central Register of Controlled Trials, Scopus, and relevant journals were searched using a combination of specific keywords ("platelet-rich fibrin," "oral surgery," and "third molar"). The final search was conducted on November 2, 2015. Randomized controlled clinical trials, as well as controlled clinical trials, aimed at comparing the effect of PRF versus natural healing after extraction of mandibular third molars were included. Five randomized controlled trials and one controlled clinical trial were included. There were 335 extractions (168 with PRF and 167 controls) in 183 participants. Considerable heterogeneity in study characteristics, outcome variables, and estimated scales was observed. Positive results were generally recorded for pain, trismus, swelling, periodontal pocket depth, soft tissue healing, and incidence of localized osteitis, but not in all studies. However, no meta-analysis could be conducted for such variables because of the different measurement scales used. The qualitative and meta-analysis results showed no significant improvement in bone healing with PRF-treated sockets compared with the naturally healing sockets. Within the limitations of the available evidence, PRF seems to have no beneficial role in bone healing after extraction of the mandibular third molars. Future standardized randomized controlled clinical trials are required to estimate the effect of PRF on socket regeneration. Copyright © 2017 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  14. The influence of premature extractions of primary molars on the ultimate root length of their permanent successors.

    Science.gov (United States)

    Brin, I; Koyoumdijsky-Kaye, E

    1981-06-01

    Final root length of lower premolars which succeed prematurely--extracted primary molars is shortened. The proposed explanation focuses on a possible accelerated movement of the permanent tooth bud and undue environmental stress following the premature extraction of its deciduous predecessor. The degree of shortening is different in both sexes and depends on the age at which the premature extraction is performed. Girls are more affected than boys, especially in cases in which the premature extractions are performed before the age of eight yr.

  15. Piezoelectric Versus Conventional Rotary Techniques for Impacted Third Molar Extraction: A Meta-analysis of Randomized Controlled Trials.

    Science.gov (United States)

    Jiang, Qian; Qiu, Yating; Yang, Chi; Yang, Jingyun; Chen, Minjie; Zhang, Zhiyuan

    2015-10-01

    Impacted third molars are frequently encountered in clinical work. Surgical removal of impacted third molars is often required to prevent clinical symptoms. Traditional rotary cutting instruments are potentially injurious, and piezosurgery, as a new osteotomy technique, has been introduced in oral and maxillofacial surgery. No consistent conclusion has been reached regarding whether this new technique is associated with fewer or less severe postoperative sequelae after third molar extraction.The aim of this study was to compare piezosurgery with rotary osteotomy techniques, with regard to surgery time and the severity of postoperative sequelae, including pain, swelling, and trismus.We conducted a systematic literature search in the Cochrane Library, PubMed, Embase, and Google Scholar.The eligibility criteria of this study included the following: the patients were clearly diagnosed as having impacted mandibular third molars; the patients underwent piezosurgery osteotomy, and in the control group rotary osteotomy techniques, for removing impacted third molars; the outcomes of interest include surgery time, trismus, swelling or pain; the studies are randomized controlled trials.We used random-effects models to calculate the difference in the outcomes, and the corresponding 95% confidence interval. We calculated the weighted mean difference if the trials used the same measurement, and a standardized mean difference if otherwise.A total of seven studies met the eligibility criteria and were included in our analysis. Compared with rotary osteotomy, patients undergoing piezosurgery experienced longer surgery time (mean difference 4.13 minutes, 95% confidence interval 2.75-5.52, P included randomized controlled trials and the sample size of each trial were relatively small, double blinding was not possible, and cost analysis was unavailable due to a lack of data.Our meta-analysis indicates that although patients undergoing piezosurgery experienced longer surgery time

  16. The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars.

    Science.gov (United States)

    Chen, Bo; Sun, Hai-Hua; Wang, Han-Guo; Kong, Hui; Chen, Fa-Ming; Yu, Qing

    2012-07-01

    Human platelet lysate (PL) has been suggested as a substitute for fetal bovine serum (FBS) in the large-scale expansion of dental pulp stem cells (DPSCs). However, the biological effects and the optimal concentrations of PL for the proliferation and differentiation of human DPSCs remain unexplored. We isolated and expanded stem cells from the dental pulp of extracted third molars and evaluated the effects of PL on the cells' proliferative capacity and differentiation potential in vitro and in vivo. Before testing, immunocytochemical staining and flow cytometry-based cell sorting showed that the cells derived from human dental pulp contained mesenchymal stem cell populations. Cells were grown on tissue culture plastic or on hydroxyapatite-tricalcium phosphate (HA/TCP) biomaterials and were incubated with either normal or odontogenic/osteogenic media in the presence or absence of various concentrations of human PL for further investigation. The proliferation of DPSCs was significantly increased when the cells were cultured in 5% PL under all testing conditions (P biomaterials and had fully covered the surface of the scaffold with an extensive sheet-like structure 14 d after seeding. In addition, 5% PL showed significantly positive effects on tissue regeneration in two in vivo transplantation models. We conclude that the appropriate concentration of PL enhances the proliferation and mineralized differentiation of human DPSCs both in vitro and in vivo, which supports the use of PL as an alternative to FBS or a nonzoonotic adjuvant for cell culture in future clinical trials. However, the elucidation of the molecular complexity of PL products and the identification of both the essential growth factors that determine the fate of a specific stem cell and the criteria to establish dosing require further investigation.

  17. Compliance of postoperative instructions following the surgical extraction of impacted lower third molars: A randomized clinical trial

    OpenAIRE

    Alvira González, Joaquín; Gay Escoda, Cosme

    2014-01-01

    Objectives: The understanding and adherence to postoperative care instructions are factors that influence the recuperation process after any surgical procedure. The aim of this study was to determine the percentage of patients who strictly follow the postoperative instructions after the extraction of an impacted lower third molar in relation to sociocultural level, preoperative anxiety scores and how postoperative information is provided to the patient. Study Design: Patients were randomly as...

  18. High-resolution dental magnetic resonance imaging of inferior alveolar nerve responses to the extraction of third molars

    Energy Technology Data Exchange (ETDEWEB)

    Kress, B.; Stippich, C.; Sartor, K. [Department of Neurology, Division of Neuroradiology, University of Heidelberg, Medical Center, 69120, Heidelberg (Germany); Gottschalk, A.; Baehren, W. [Department of Radiology, Armed Forces Hospital, Ulm (Germany); Anders, L.; Palm, F. [Department of Dental, Oral and Maxillofacial Surgery, Armed Forces Hospital, Ulm (Germany)

    2004-08-01

    The objective of this study was to assess whether signal changes can be detected in the neurovascular bundle of the mandibular canal after the extraction of a third molar. We retrospectively analyzed MRI scans of 30 test subjects with healthy mandibles and 41 patients who had had a wisdom tooth extracted. Signal intensities were measured at particular sites in the neurovascular bundle, which were defined as regions of interest (ROI) in the sagittal T1-weighted images before and after intravenous administration of a paramagnetic contrast agent. On the basis of the signal intensity increases that were measured after contrast agent administration, we compared the signal increases obtained for the patients who had received surgical treatment with the results obtained for the population of test subjects with unremarkable mandibles (t-test, P<0.05). Compared with the healthy test subjects, patients who had received surgical treatment showed significantly higher signal intensity increases at two measurement sites, i.e., the second molar and the second premolar (P<0.05). We found no significant differences when the measurements were performed at the first molar (P=0.06), the third molar (P=0.47) and in the area of the ascending mandibular ramus (P=0.79). Compared with a population of healthy test subjects, patients who had their third molars surgically removed show higher signal intensity increases in the neurovascular bundle after intravenous contrast agent administration. The underlying cause may be the higher blood flow in the arteries and veins and the perineural plexus, which may give evidence of the pathophysiological mechanism of nerve damage in the narrow canal as a result of osteotomy. (orig.)

  19. A Four-Year Monocentric Study of the Complications of Third Molars Extractions under General Anesthesia: About 2112 Patients

    Directory of Open Access Journals (Sweden)

    A. Guerrouani

    2013-01-01

    Full Text Available Introduction. The aim of this study was to assess the complications resulting from third molar extraction under general anesthesia. Material and Methods. The retrospective study included all patients who underwent impacted third molars extraction from January 2008 until December 2011. 7659 third molars were extracted for 2112 patients. Postoperative complications were retrieved from medical files. Results. No complications were related to general anesthesia. The most frequent postoperative complication was infection (7.15%. Lingual nerve injuries affected 1.8% of the patients. All of them were transient and were not related to tooth section. Inferior alveolar nerve injuries were reported in 0.4% of the cases. 95.8% of these patients were admitted for one-day ambulatory care, and only two patients were readmitted after discharge from hospital. Discussion. This surgical technique offers comfort for both surgeons and patients. Risks are only linked to the surgical procedure as we observed no complication resulting from general anesthesia. One-day hospitalization offers a good balance between comfort, security, and cost. The incidence of complications is in agreement with the literature data, especially regarding pain, edema, and infectious and nervous complications. It is of utmost importance to discuss indications with patients, and to provide them with clear information.

  20. Enamel thickness variation of deciduous first and second upper molars in modern humans and Neanderthals.

    Science.gov (United States)

    Fornai, Cinzia; Benazzi, Stefano; Svoboda, Jiří; Pap, Ildikó; Harvati, Katerina; Weber, Gerhard W

    2014-11-01

    Enamel thickness and dental tissue proportions have been recognized as effective taxonomic discriminators between Neanderthal and modern humans teeth. However, most of the research on this topic focused on permanent teeth, and little information is available for the deciduous dentition. Moreover, although worn teeth are more frequently found than unworn teeth, published data for worn teeth are scarce and methods for the assessment of their enamel thickness need to be developed. Here, we addressed this issue by studying the 2D average enamel thickness (AET) and 2D relative enamel thickness (RET) of Neanderthal and modern humans unworn to moderately worn upper first deciduous molars (dm(1)s) and upper second deciduous molars (dm(2)s). In particular, we used 3D μCT data to investigate the mesial section for dm(1)s and both mesial and buccal sections for dm(2)s. Our results confirmed previous findings of an Neanderthal derived condition of thin enamel, and thinner enamel in dm(1)s than dm(2)s in both Neanderthal and modern humans. We demonstrated that the Neanderthal 2D RET indices are significantly lower than those of modern humans at similar wear stages in both dm(1)s and dm(2)s (p Neanderthal unworn to moderately worn upper deciduous molars.

  1. A randomized comparative prospective study of platelet-rich plasma, platelet-rich fibrin, and hydroxyapatite as a graft material for mandibular third molar extraction socket healing.

    Science.gov (United States)

    Dutta, Shubha Ranjan; Passi, Deepak; Singh, Purnima; Sharma, Sarang; Singh, Mahinder; Srivastava, Dhirendra

    2016-01-01

    The purpose of this study was to compare the efficacy of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and hydroxyapatite (HA) for reduction of pain and swelling, absence of dry socket, soft tissue healing, and bone regeneration after mandibular third molar extraction in human patients. Forty patients requiring extraction of mandibular third molars were randomly grouped as control, PRP, PRF, and HA-treated. The patients were assessed for postoperative pain, swelling, dry socket, and soft tissue healing on the 3(rd), 7(th), and 14(th) day of postoperative periods depending on the standard methods. Radiological assessment of the extraction site was done at 1, 2, and 6 months interval to compare the change in bone density in the sockets in control and treated patients. Pain and swelling were less on PRP and PRF site when compared to HA and control site. PRP and PRF site showed better soft tissue healing when compared to HA and control site. Radiographic assessment showed comparatively lesser bone density values in PRP, PRF, and control site at 1, 2, and 6 months than HA site. Our study showed that PRP and PRF are better graft materials than HA regarding pain, swelling, dry socket, and soft tissue healing. Bone regeneration is induced promptly by HA as compared to other graft materials. However, a more elaborate study with a larger number of clinical cases is very much essential to be more conclusive regarding the efficacy of the graft materials.

  2. Observations on pulpal response to carbon dioxide laser drilling of dentine in healthy human third molars.

    Science.gov (United States)

    Nair, P N R; Baltensperger, M; Luder, H U; Eyrich, G K H

    2005-01-01

    Preservation of pulpal health is the primary prerequisite for successful application of laser systems in the hard tissue management of vital teeth. The purpose of this study was to investigate the short and long-term pulpal effects to cavity preparations in healthy human teeth using carbon dioxide (CO2) laser. A total of seven, healthy, third molars that were scheduled to be removed due to space problems were used. After the laser drilling, the occlusal cavities were closed temporarily, and the teeth were extracted 7 days (n=5) and 3 months (n=2) after the operation. The specimens were fixed, decalcified, subdivided and processed for light and transmission electron microscopy. Seven days postoperatively all the five teeth that had been irradiated with the CO2 laser did not reveal any pathological changes in the pulpo-dentine complex. Three months postoperatively the two teeth that were prepared with the laser showed subtle but distinct apposition of tertiary dentine that was lined with intact odontoblasts. One of the specimens at 3 months revealed the presence of a mild, but very circumscribed, pulpal infiltration of chronic inflammatory cells subjacent to the cavity preparation. The latter is unlikely to be due to a direct effect of the laser irradiation but a possible consequence of microleakage of oral antigens and/or other tissue-irritating molecules through the temporary restoration and the remaining dentine thickness (RDT). Although these preliminary histological results suggest that the CO2 laser under investigation induced only minimal response of the dentine-pulp complex when used as a hard-tissue drilling tool, with specific energy settings, pulse duration within thermal relaxation time and emitting radiations at 9.6 microm of wavelength, larger clinical trials involving various types of teeth are necessary to reach definite conclusions for large-scale clinical application of the laser device.

  3. Correlation of antibiotic prophylaxis and difficulty of extraction with postoperative inflammatory complications in the lower third molar surgery.

    Science.gov (United States)

    Lee, J Y; Do, H S; Lim, J H; Jang, H S; Rim, J S; Kwon, J J; Lee, E S

    2014-01-01

    Our aim was to investigate the correlation among antibiotic prophylaxis, difficulty of extraction, and postoperative complications in the removal of lower 3rd molars. A total of 1222 such extractions in 890 patients between January 2010 and January 2012 were analysed retrospectively. The difficulty of extraction measured by Pederson's index, antibiotic prophylaxis with cefditoren, and postoperative complications were recorded. The difficulty of extraction was significantly associated with postoperative complications (p=0.03). There were no significant associations between antibiotic prophylaxis and postoperative complications in groups of equal difficulty ("easy" group (class I) p=1.00; "moderate" group (class II) p=1.00; and "difficult" group (class III) p=0.65). There was a small but insignificant increase in the number of dry sockets and infections in class III cases. In conclusion, this study provides further evidence that antibiotic prophylaxis for the prevention of postoperative inflammatory complications is unnecessary for extraction of 3rd molars. Copyright © 2013. Published by Elsevier Ltd.

  4. Orthodontic Extraction of High-Risk Impacted Mandibular Third Molars in Close Proximity to the Mandibular Canal: A Systematic Review.

    Science.gov (United States)

    Kalantar Motamedi, Mahmood Reza; Heidarpour, Majid; Siadat, Sara; Kalantar Motamedi, Alimohammad; Bahreman, Ali Akbar

    2015-09-01

    Extraction of mandibular third molars (M3s) in close proximity to the mandibular canal has some inherent risks to adjacent structures, such as neurologic damage to teeth, bone defects distal to the mandibular second molar (M2), or pathologic fractures in association with enlarged dentigerous cysts. The procedure for extrusion and subsequent extraction of high-risk M3s is called orthodontic extraction. This is a systematic review of the available approaches for orthodontic extraction of impacted mandibular M3s in close proximity to the mandibular canal and their outcomes. The PubMed, Scopus, Cochrane Central Register of Controlled Trials (CENTRAL), DOAJ, Google Scholar, OpenGrey, Iranian Science Information Database (SID), Iranmedex, and Irandoc databases were searched using specific keywords up to June 2, 2014. Studies were evaluated based on predetermined eligibility criteria, treatment approaches, and their outcomes. Thirteen articles met the inclusion criteria. A total of 123 impacted teeth were extracted by orthodontic extraction and 2 cases were complicated by transient paresthesia. Three types of biomechanical approaches were used: 1) using the posterior maxillary region as the anchor for orthodontic extrusion of lower M3s, 2) simple cantilever springs attached to the M3 buttonhole, and 3) cantilever springs tied to a bonded orthodontic bracket on the M3 plus multiple-loop spring wire for distal movement of the M3. Osteo-periodontal status of M2s also improved uneventfully. Despite the drawbacks of orthodontic extraction, removal of deeply impacted M3s using the described techniques is safe with regard to mandibular nerve injury and neurologic damage. Orthodontic extraction is recommended for extraction of impacted M3s that present a high risk of postoperative osteo-periodontal defects on the distal surface of the adjacent M2 and those associated with dentigerous cysts. Copyright © 2015 American Association of Oral and Maxillofacial Surgeons. Published by

  5. Efficacy of hyaluronic acid spray on swelling, pain, and trismus after surgical extraction of impacted mandibular third molars.

    Science.gov (United States)

    Koray, M; Ofluoglu, D; Onal, E A; Ozgul, M; Ersev, H; Yaltirik, M; Tanyeri, H

    2014-11-01

    The aim of this study was compare the efficacies of two oral sprays in reducing swelling, pain, and trismus after the extraction of impacted mandibular third molars. This prospective double-blind, randomized, crossover clinical trial included 34 patients with bilateral symmetrically impacted mandibular third molars of similar surgical difficulty. Hyaluronic acid or benzydamine hydrochloride spray was applied (two pumps) to the extraction area, three times daily for 7 days. Swelling was evaluated using a tape measure method, pain with a visual analogue scale (VAS), and trismus by measuring the maximum inter-incisal opening. Assessments were made on the day of surgery and on days 2 and 7 after surgery. Statistically significant differences were detected for the swelling and trismus values between the two treatment groups on the second postoperative day (P=0.002 and P=0.03, respectively). However, there was no statistically significant difference in VAS scores between the two groups. The administration of hyaluronic acid spray was more effective than benzydamine hydrochloride spray in reducing swelling and trismus. Although no evidence of a reduction in pain levels was detected, hyaluronic acid appears to offer a beneficial effect in the management of swelling and trismus during the immediate postoperative period following impacted third molar surgery. Copyright © 2014 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  6. Overjet correction and space closure mechanisms for Class II treatment by extracting the maxillary first molars.

    Science.gov (United States)

    Booij, Johan W; Goeke, Juliane; Bronkhorst, Ewald M; Pancherz, Hans; Ruf, Sabine; Katsaros, Christos

    2011-07-01

    To analyze the mechanism of overjet correction and space closure when treating Class II Division 1 patients by extracting the maxillary first molars. A total of 100 prospective, consecutively treated Class II Division 1 patients (45 female, 55 male; 10.5-17.2 years old). Standardized lateral cephalograms prior to and after treatment were evaluated via a modified sagittal occlusion analysis (SO analysis). The mean degree of overjet correction was 5.2 mm (95% CI = 4.8-5.6 mm) and was on average achieved by means of 1.7 mm skeletal and 3.5 mm dental changes. The relationship between the premolars improved by 4.8 mm toward a Class I relationship, facilitated by 1.7 mm skeletal and 3.1 mm dental changes. The 11.3 mm space closure in the maxillary first-molar extraction area resulted from distalization of the second premolars (1.4 mm) and a mesialization of the second molars (9.9 mm). Overjet correction was essentially achieved by a retrusion of the upper incisors, as well as by ventral growth of the lower jaw and protrusion of the lower incisors. Space closure was only partly achieved by distalization of the premolars.

  7. Effect of a combination of oral midazolam and low-dose ketamine on anxiety, pain, swelling, and comfort during and after surgical extractions of mandibular third molars

    Directory of Open Access Journals (Sweden)

    Rubina Gupta

    2012-01-01

    Conclusions: Premedication with midazolam plus low-dose ketamine prior to surgical extraction of third molars can provide the patient with a comfortable procedure and good postoperative analgesia, with less swelling and significantly less pain.

  8. Formocresol versus calcium hydroxide direct pulp capping of human primary molars: two year follow-up.

    Science.gov (United States)

    Aminabadi, Naser Asl; Farahani, Ramin Mostofi Zadeh; Oskouei, Sina Ghertasi

    2010-01-01

    Clinical and radiographic evaluation of the premedicated direct pulp capping using formocresol (PDC) versus conventional direct pulp capping using calcium hydroxide (CDC) in human carious primary molars. A total of 120 vital primary molars with pinpoint exposure during caries removal in 84 patients aged 4-5 years were selected. In the PDC group (n = 60), 20% Buckley's formocresol solution, and in the CDC group (n = 60), calcium hydroxide powder were applied to the exposure sites followed by placement of zinc oxide-eugenol base. Teeth were restored with preformed stainless steel crowns. Clinical and radiographic evaluations of the treatment outcomes were performed at regular intervals of 6 and 12 months, respectively, for two years post-operatively. The prevalence of spontaneous pain, sensitivity on percussion, and fistula were significantly higher in the CDC group compared to the PDC group (P formocresol premedicated direct pulp capping could safely be used as a substitute for conventional direct pulp capping.

  9. Surgical Protocol and Short-Term Clinical Outcome of Immediate Placement in Molar Extraction Sockets Using a Wide Body Implant

    Directory of Open Access Journals (Sweden)

    Stefan Vandeweghe

    2011-08-01

    Full Text Available Objectives: Implant placement in molar extraction sockets can be difficult due to complex multi-root anatomy and the lack of predictable primary stability. The aim of this study was to evaluate the outcome of an 8 - 9 mm diameter tapered implant, designed to be placed in molar extraction sockets.Material and methods: Patients treated at least 1 year before with a Max® implant (Southern Implants, Irene, South Africa were invited for a clinical examination. Variables collected were surgical and prosthetic protocol, implant dimension and smoking habits. Peri-implant bone level was determined on peri-apical radiographs and compared to baseline, being implant insertion.Results: 98 implants had been placed in 89 patients. One implant had failed. Thirty eight patients representing 47 implants (maxilla 26, mandible 21 were available for clinical examination. Mean bone loss was 0.38 mm (SD 0.48; range - 0.50 – 1.95 after a mean follow-up of 20 months (range 12 - 35. Implant success was 97.9%. Around 30 implants, a bone substitute was used to fill the residual space, but this did not affect the bone loss outcome. Bone loss was only significantly different between maxilla and mandible (0.48 mm vs. 0.27 mm and between the 8 and 9 mm diameter implants (0.23 mm vs. 0.55 mm. A full papilla was present at 71% of the interproximal sites and irrespective of bone loss.Conclusions: The Max® implant demonstrated good primary stability, when placed in molar extraction sockets, with limited bone loss over time.

  10. Diagnostic value of panoramic radiography in predicting inferior alveolar nerve injury after mandibular third molar extraction: a meta-analysis.

    Science.gov (United States)

    Liu, W; Yin, W; Zhang, R; Li, J; Zheng, Y

    2015-06-01

    The aim of this study was to evaluate the predictive value of panoramic radiography on inferior alveolar nerve (IAN) injury after extraction of the mandibular third molar. Relevant studies up to 1 June 2014 that discussed the association of panoramic radiography signs and post-mandibular third molar extraction IAN injury were systematically retrieved from the databases of PubMed, Embase, Springerlink, Web of Science and Cochrane library. The effect size of pooled sensitivity, specificity, positive likelihood ratios (PLR), negative likelihood ratios (NLR) and diagnostic odds ratio (DOR) with their 95% confidence intervals (CI) were statistically analysed with Meta-disc 1.4 software. Nine articles were included in this meta-analysis. The pooled estimates of sensitivity and specificity were 0.56 (95% CI: 0.50-0.61) and 0.86 (95% CI: 0.84-0.87), respectively. The overall PLR was 3.46 (95% CI: 2.02-5.92) and overall NLR was 0.58 (95% CI: 0.45-0.73). The pooled estimate of DOR was 6.49 (95% CI: 2.92-14.44). The area under the summary receiver operating characteristic curve was 0.7143 ± 0.0604. The meta-analysis indicated that interpretation of panoramic radiography based on darkening of the root had a high specificity in predicting IAN injury after mandibular third molar extraction. However, the ability of this panoramic radiography marker to detect true positive IAN injury was not satisfactory. © 2015 Australian Dental Association.

  11. [Systematic review on control of swelling and trismus after extraction of impacted mandibular third molar by dexamethasone pericoronal injection].

    Science.gov (United States)

    Li, Chunjie; Zhao, Hongwei; Li, Longjiang; Li, Shuangjun; Wu, Yuan; Liao, Xuejuan; Pan, Jian

    2013-06-01

    To assess the efficacy of Dexamethasone (DM) pericoronal injection for the control of swelling and trismus caused by impacted mandibular third molars extraction. Cochrane, PUBMED, EMBASE and CBM were searched for eligible studies. Hand-searching included references of the included studies and Chinese dental journals. Risk of bias of the included studies was assessed by two reviewers independently using Cochrane Collaboration's tool, and data extraction was done by them. Meta-analysis was delivered with Revman 5.1. Seven randomized controlled trials, involving 684 participants, were included. Six of them had moderate risk of bias and one had high risk of bias. Meta-analysis showed that DM pericoronal injection could relieve trismus by 6.77 mm (P=0.02) within 1-2 days after the surgery. It could also reduce 51% of the risk of moderate-severe trismus(P0.05). Periodontal injection of 4-5 mg DM could control facial swelling and trismus following impacted mandibular third molar extraction. But more randomized controlled trials are needed.

  12. Comparative Assessment of Preoperative versus Postoperative Dexamethasone on Postoperative Complications following Lower Third Molar Surgical Extraction

    Directory of Open Access Journals (Sweden)

    Hashem M. Al-Shamiri

    2017-01-01

    Full Text Available Aim. To evaluate the effect of preoperative versus postoperative administration of oral Dexamethasone on postoperative complications including pain, edema, and trismus following lower third molar surgery. Methods. 24 patients were divided into two equal groups receiving 8 mg Dexamethasone orally, one group one hour preoperatively and the other group immediately after surgery. Pain was measured using VAS, edema was measured using a graduated tape between 4 fixed points in the face, and the mouth opening was measured using a graduated sliding caliper. Results. In this study pain and trismus records were similar and statistically nonsignificant in both groups. The results had proven that preoperative administration was superior when compared to postoperative administration regarding edema (0.002. Conclusions. Preoperative oral administration of 8 mg Dexamethasone was superior to the postoperative administration of the same dose concerning edema after lower third molar surgery.

  13. Effect of low-level laser therapy after extraction of impacted lower third molars.

    Science.gov (United States)

    Ferrante, Maurizio; Petrini, Morena; Trentini, Paolo; Perfetti, Giorgio; Spoto, Giuseppe

    2013-05-01

    The aim of this study is to evaluate the effectiveness of the low-level laser therapy (LLLT) in the control of pain, swelling, and trismus associated with surgical removal of impacted lower third molars. Thirty patients were randomized into two treatment groups, each with 15 patients-group test (LLLT) and a group control (no-LLLT)-and were told to avoid any analgesics 12 h before the procedure. In group test, the 980-nm diode-laser (G-Laser 25 Galbiati, Italy) was applied, using a 600-μm handpiece, intraorally (lingual and vestibular) at 1 cm from the involved area and extraoral at the insertion point of the masseter muscle immediately after surgery and at 24 h. The group control received only routine management. Parameters used for LLLT were: continuous mode, at 300 mW (0.3 W) for a total of 180 s (60 s × 3) (0.3 W × 180 s=54 J). Group test showed improvement in the interincisal opening and remarkable reduction of trismus, swelling and intensity of pain on the first and the seventh postoperative days. Although LLLT has been reported to prevent swelling and trismus following the removal of impacted third molars, some of these studies reported a positive laser effect while others did not. All references to the use of laser therapy in the postoperative management of third molar surgery employ different methodologies and, in some, explanations as to selection of their respective radiation parameters are not given. This study has demonstrated that LLLT, with these parameters, is useful for the reduction of postoperative discomfort after third-molar surgery.

  14. Surgical extraction of impacted inferior third molars at risk for inferior alveolar nerve injury.

    Science.gov (United States)

    Gallesio, Cesare; Berrone, Mattia; Ruga, Emanuele; Boffano, Paolo

    2010-11-01

    The objective of the study was to prospectively assess the intraoperative findings and the radiographic signs of a study population of patients with impacted third molars at risk of inferior alveolar nerve injury. One hundred thirty-four patients with impacted mandibular third molars at risk for nerve injury were included in the study. Radiographic signs of possible close relationship between the 2 structures and intraoperative exposition or damage of the inferior alveolar nerve were recorded. The follow-up controls comprised clinical examinations and assessment for the sensation of the lower lip and chin. In 24 patients, a real contiguity was encountered between the third molars and nerve. Among these, intraoperative neural exposition was observed in 19 patients. Four patients complained of postoperative temporary hypoesthesia. No deficit of sensibility was found in cases with no exposition of the nerve. The accidental exposition of the inferior alveolar nerve is associated with an increased risk for neural injuries. At panoramic radiograph, the presence of signs of bifid and radiolucent apex, superimposition, and radiolucent root band should be considered at high risk for neural damage.

  15. Retention and extraction of the third molar in orthodontic clinic%正畸和第三磨牙的保留与拔除

    Institute of Scientific and Technical Information of China (English)

    崔敏娟; 常新

    2015-01-01

    Impaction of the third molar especially the mandibular third molar is commonly seen in the dental clinic.Or-thodontic treatment can affect the condition of the third molar, and then the choice of retention or extraction of the third mo-lar.Orthodontic treatment can upright impacted third molar and achieve the retention of third molar.On the other hand, or-thodontic treatment also can be an auxiliary to the extraction of impacted third molar.This paper summarizes the research progress of the relationship between orthodontics and the retention or extraction of the third molar.%临床上第三磨牙尤其是下颌第三磨牙常见阻生。正畸治疗能对第三磨牙的状态产生影响,进而影响对第三磨牙保留与拔除的选择。临床上可以通过正畸治疗直立第三磨牙,实现第三磨牙的保留,也可以通过正畸的方法辅助第三磨牙的拔除。本文就正畸和第三磨牙的保留与拔除之间的关系的研究进展进行综述。

  16. Effectiveness of a personalized device in the evaluation of mandibular second molar periodontal healing after surgical extraction of adjacent third molar

    National Research Council Canada - National Science Library

    Pippi, Roberto

    2013-01-01

    The primary aim of the present study was to validate the effectiveness of a personalized device able to guide periodontal probing in evaluation of second molar periodontal healing after adjacent third...

  17. Dental caries, restorations and extractions by dental caries in first permanent molars. Clinical and radiographic study; Lesoes cariosas, restauracoes e extracoes por processo carioso de primeiros molares permanentes. Estudo clinico e radiografico

    Energy Technology Data Exchange (ETDEWEB)

    Aguiar, Sandra Maria H.C. Avila de; Santos Pinto, Ruy dos [UNESP, Aracatuba, SP (Brazil). Faculdade de Odontologia

    1996-07-01

    This research analyse by clinical and radiographic study, dental caries, restorations and extractions in 1.600 first permanent molars, from 400 children, both sexes, aged 5 to 13 years old, assisted in the Children`s Clinic, Faculdade de Odontologia de Aracatuba, UNESP, in 1994. (author). 3 refs., 4 figs.

  18. Clinician-related factors behind the decision to extract an asymptomatic lower third molar. A cross-sectional study based on Spanish and Portuguese dentists.

    Science.gov (United States)

    Alves-Pereira, D; Pereira-Silva, D; Figueiredo, R; Gay-Escoda, C; Valmaseda-Castellón, E

    2017-09-01

    Scientific literature estimates that around 18 to 40 % of asymptomatic third molars are extracted. The aims of the present study were to determine the indications for extraction of asymptomatic lower third molars in a sample of Spanish and Portuguese dentists, and to relate these indications to the clinicians' training and professional experience. A survey consisting of 15 cases of asymptomatic lower third molars was emailed to Portuguese and Spanish dentists. The clinicians were asked to assess the level of difficulty of the extractions and to make a reasoned recommendation based on the panoramic radiographs, gender and age of the patients. 381 clinicians filled in the questionnaires. Most of the professionals had over 13 years of clinical experience. The number of Spanish clinicians with postgraduate degrees in Oral Surgery was significantly higher. On average, 42% of respondents recommended extraction of asymptomatic third molars. The indication for extraction was significantly higher among Portuguese dentists. Clinical experience was negatively correlated with the perceived extraction difficulty (pPortuguese dentists were more in favour of removing asymptomatic lower third molars than the Spanish dentists, although the latter had a higher proportion of professionals with postgraduate studies in Oral Surgery.

  19. Clinical and radiographic evaluation of the effects of guided tissue regeneration using resorbable membranes after extraction of impacted mandibular third molars.

    Science.gov (United States)

    Aimetti, Mario; Pigella, Enrico; Romano, Federica

    2007-02-01

    The aim of this investigation was to evaluate the healing following guided tissue regeneration of bone defects distal to the mandibular second molars after surgical extraction of soft tissue-impacted, mesioangularly inclined third molars. After the impacted third molars were extracted, 15 sites were treated with polyglycolic acid/polylactic acid bioresorbable membranes plus scaling and root planing (SRP) of the distal surface of the second molars (test sites); 15 sites were treated with SRP alone (control sites) according to a split-mouth design. At 12 months after surgery, the placement of barrier material was shown to have resulted in statistically significant differences in clinical attachment level and bone gain compared to control sites.

  20. 拔除病变第一磨牙正畸治疗的临床体会%Clinical effect of orthodontic treatment with first molar extraction

    Institute of Scientific and Technical Information of China (English)

    吴欣; 刘国元

    2011-01-01

    27 patients with severe lesion in first molar were received first molar extraction followed by orthodontic treatment.Satisfactory effects were obtained.%27 例伴有第一磨牙严重病变的正畸拔牙患者,通过拔除病变第一磨牙代替常规拔牙进行治疗,取得了良好矫治效果,总结该类患者的治疗要点.

  1. Influence of two different surgical techniques on the difficulty of impacted lower third molar extraction and their post-operative complications

    OpenAIRE

    Mavrodi, Alexandra; Ohanyan, Ani; Kechagias, Nikos; Antonis TSEKOS; Konstantinos VAHTSEVANOS

    2015-01-01

    Background Post-operative complications of various degrees of severity are commonly observed in third molar impaction surgery. For this reason, a surgical procedure that decreases the trauma of bone and soft tissues should be a priority for surgeons. In the present study, we compare the efficacy and the post-operative complications of patients to whom two different surgical techniques were applied for impacted lower third molar extraction. Material and Methods Patients of the first group unde...

  2. Maxillary sinus floor extension and posterior tooth inclination in adolescent patients with Class II Division 1 malocclusion treated with maxillary first molar extractions.

    Science.gov (United States)

    Livas, Christos; Halazonetis, Demetrios J; Booij, Johan Willem; Pandis, Nikolaos; Tu, Yu-Kang; Katsaros, Christos

    2013-04-01

    Our objective was to investigate potential associations between maxillary sinus floor extension and inclination of maxillary second premolars and second molars in patients with Class II Division 1 malocclusion whose orthodontic treatment included maxillary first molar extractions. The records of 37 patients (18 boys, 19 girls; mean age, 13.2 years; SD, 1.62 years) treated between 1998 and 2004 by 1 orthodontist with full Begg appliances were used in this study. Inclusion criteria were white patients with Class II Division 1 malocclusion, sagittal overjet of ≥4 mm, treatment plan including extraction of the maxillary first permanent molars, no missing teeth, and no agenesis. Maxillary posterior tooth inclination and lower maxillary sinus area in relation to the palatal plane were measured on lateral cephalograms at 3 time points: at the start and end of treatment, and on average 2.5 years posttreatment. Data were analyzed for the second premolar and second molar inclinations by using mixed linear models. The analysis showed that the second molar inclination angle decreased by 7° after orthodontic treatment, compared with pretreatment values, and by 11.5° at the latest follow-up, compared with pretreatment. There was evidence that maxillary sinus volume was negatively correlated with second molar inclination angle; the greater the volume, the smaller the inclination angle. For premolars, inclination increased by 15.4° after orthodontic treatment compared with pretreatment, and by 8.1° at the latest follow-up compared with baseline. The volume of the maxillary sinus was not associated with premolar inclination. We found evidence of an association between maxillary second molar inclination and surface area of the lower sinus in patients treated with maxillary first molar extractions. Clinicians who undertake such an extraction scheme in Class II patients should be aware of this potential association and consider appropriate biomechanics to control root

  3. Evaluation of the indication for surgical extraction of third molars according to the oral surgeon and the primary care dentist. Experience in the Master of Oral Surgery and Implantology at Barcelona University Dental School

    OpenAIRE

    Fuster Torres, M. Angeles; Gargallo Albiol, Jordi; Berini Aytés, Leonardo; Gay Escoda, Cosme

    2008-01-01

    Introduction: Third molar extraction is the most frequent procedure in oral surgery. The present study evaluates the indication of third molar extraction as established by the primary care dentist (PCD) and the oral surgeon, and compares the justification for extraction with the principal reason for patient consultation. Patients and method: A descriptive study was made of 319 patients subjected to surgical removal of a third molar in the context of the Master of Oral Surgery and Implantology...

  4. [Hyperthyroidism in molar pregnancy].

    Science.gov (United States)

    Boufettal, H; Mahdoui, S; Noun, M; Hermas, S; Samouh, N

    2014-03-01

    Hyperthyroidism is a rare complication of molar pregnancy. We report a 39-year-old woman who presented a thyrotoxic syndrome accompanying a molar pregnancy. Serum thyroid hormones were elevated and returned to normal level after uterine evacuation of a molar pregnancy. The authors detail the role of thyroid stimulating property of human gonadotropin chorionic hormone and its structural changes during the gestational trophoblastic diseases. These changes give the latter the thyroid stimulating properties and signs of hyperthyroidism. Molar pregnancy may be a cause of hyperthyroidism. The diagnosis of molar pregnancy should be a mention to thyrotoxicosique syndrome in a woman of childbearing age. Copyright © 2013. Published by Elsevier SAS.

  5. Má oclusão Classe I de Angle tratada com extrações de primeiros molares permanentes Angle Class I malocclusion treated with extraction of first permanent molars

    Directory of Open Access Journals (Sweden)

    Ivan Tadeu Pinheiro da Silva

    2010-08-01

    Full Text Available A má oclusão Classe I de Angle é caracterizada por uma relação anteroposterior normal nos molares, que pode ou não estar acompanhada por alterações esqueléticas - nos planos vertical ou transverso - ou dentárias. A biprotrusão, revelada pela inclinação acentuada dos incisivos superiores e inferiores para vestibular, somada ao trespasse horizontal excessivo, faz com que o paciente fique mais exposto a traumas dentários, além de causar comprometimento estético. A escolha dos dentes a serem extraídos para a correção, geralmente, recai sobre os primeiros ou segundos pré-molares, devido à sua posição na arcada. Contudo, a extração do primeiro molar permanente, em função de algum comprometimento, por cárie ou restauração extensa, pode ser uma alternativa, proporcionando a manutenção de um dente hígido em detrimento de outro já manipulado. O presente caso, tratado de maneira incomum, pela extração dos quatro primeiros molares permanentes, foi apresentado à Diretoria do Board Brasileiro de Ortodontia e Ortopedia Facial (BBO, representando a categoria 2, como parte dos requisitos para a obtenção do título de diplomado pelo BBO.Angle Class I malocclusion is characterized by normal anteroposterior molar relationship, which may or may not be accompanied by skeletal changes-in the vertical or transverse planes-or dental changes. Bimaxillary dental protrusion, characterized by pronounced labial inclination of maxillary and mandibular incisors combined with excessive overjet, expose patients to dental trauma and compromise aesthetics. In deciding which teeth to extract for Class I correction the first or second premolars are usually selected due to their location in the dental arch. However, the extraction of a first permanent molar compromised by caries or extensive restoration may be an alternative that ensures the preservation of a healthy tooth instead of one that has already been manipulated. This case, treated in

  6. In vitro evaluation of a passive radio frequency identification microchip implanted in human molars subjected to compression forces, for forensic purposes of human identification

    National Research Council Canada - National Science Library

    Moreno, Freddy; Vallejo, Diego; Garzón, Herney; Moreno, Sandra

    2013-01-01

    To evaluate the in vitro behavior of a passive Radio Frequency Identification (RFID) microchip implanted in human molars subjected to compression forces to determine its technical and clinical viability...

  7. Significance of Radiological Variables Studied on Orthopantamogram to Pridict Post-Operative Inferior Alveoler Nerve Paresthesia After Third Molar Extraction

    OpenAIRE

    Pathak, Sachin; Mishra, Nitin; Rastogi, Madhur Kant; Sharma, Shalini

    2014-01-01

    Context: Removal of impacted third molar is a procedure that is often associated with post-operative complications. The rate of complications is somewhat high because of its proximity to the vital structures. Inferior alveolar nerve paresthesia is one of the common complications of impacted their molar surgery. This is due to intimate relationship between roots of mandibular third molar and inferior alveolar canal. To access the proximity of inferior alveolar canal to third molar many diagnos...

  8. Effects of ionizing radiation on wound healing of alveolar bone socket after extraction of rat maxillary molars

    Energy Technology Data Exchange (ETDEWEB)

    Iwata, Hiroshi; Yosue, Takashi [Nippon Dental Univ., Tokyo (Japan). School of Dentistry; Nasu, Masanori

    2001-05-01

    The purpose of this study was to examine the effects of radiation on the healing process of tooth extraction wounds. X-ray doses of 10 Gy or 20 Gy were delivered, once, to the maxillofacial area of Wistar-strain rats. Then, 24 hours after irradiation, the maxillary first molars were extracted bilaterally. The animals were sacrificed 3, 7, 10, 14, 21, 42, and 84 days after tooth extraction, and the maxilla were sliced, to make thin sections. These specimens were then double stained with alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP). The ratio of bone area to socket area (bone formation ratio), the ratio of bone length to ALP positive area length (ALP positive ratio), and the number of TRAP-positive cells, were evaluated. The results showed: The bone formation ratios at days 3 and 7 after tooth extraction were significantly low in both irradiation groups, compared with those for the non-irradiation group. The ALP positive reaction ratio peaked 7 days after in the non-irradiation group. In both irradiation groups, the ratios that were worked out at 3 days and 7 days after were significantly lower than those in the non-irradiation group. There was no significant difference in the number of TRAP-positive cells between the non-irradiation group and the 10 Gy irradiation group. In the 20 Gy irradiation group, the TRAP-positive cell count plummeted to a significantly low level at 3 days after tooth extraction, compared with that in the non-irradiation group. (author)

  9. Efficacy of a single dose of low-level laser therapy in reducing pain, swelling, and trismus following third molar extraction surgery.

    Science.gov (United States)

    Landucci, A; Wosny, A C; Uetanabaro, L C; Moro, A; Araujo, M R

    2016-03-01

    The clinical efficacy of low-level laser therapy (LLLT) for the reduction of pain, swelling, and trismus following the surgical extraction of third molars was evaluated. Mandibular third molars, with similar radiographic positions on two distinct sections, were extracted from 22 patients. Immediately after extraction from the randomly selected right or left side, LLLT was applied (study group). The same extraction procedure was performed 21 days later on the other third molar, without the application of LLLT (control group). LLLT was applied at 10 points: four intraoral in close proximity to the socket and six extraoral along the masseter muscle. Pain intensity was assessed using a visual analogue scale, swelling was measured as the distance from the tragus to the median base of the mentum, and trismus was assessed by the extent of mouth opening. Data were collected at four time points: before surgery, immediately after surgery, 48h postoperatively, and 7 days postoperatively. Compared with the control group, the study group showed significant reductions in pain, swelling, and trismus at 48h and 7 days postoperatively. In conclusion, a single dose of LLLT was effective at reducing the postoperative discomforts (pain, swelling, and trismus) associated with third molar extraction surgery. Copyright © 2015 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  10. Paediatric necrotizing fasciitis complicating third molar extraction: report of a case.

    Science.gov (United States)

    Ricalde, P; Engroff, S L; Jansisyanont, P; Ord, R A

    2004-06-01

    Necrotizing fasciitis is an uncommon but well-described entity. In the paediatric population compromising risk factors are frequently absent. We describe the successful treatment of a case of cervicofacial necrotizing fasciitis in a healthy 14-year-old male following routine extraction of an uninfected wisdom tooth for orthodontic purposes.

  11. Avaliação radiográfica do comportamento dos terceiros molares inferiores em pacientes tratados ortodonticamente com e sem extrações de primeiros pré-molares Radiographic evaluation of the behavior of lower third molars in patients that were orthodontically treated with and without first premolars extractions

    Directory of Open Access Journals (Sweden)

    Reinaldo Roberto Hauy

    2007-12-01

    Full Text Available OBJETIVO: o presente trabalho objetivou avaliar as alterações ocorridas nas posições dos terceiros molares inferiores em pacientes tratados ortodonticamente com extrações dos primeiros pré-molares. METODOLOGIA: utilizou-se 80 ortopantomografias, obtidas no pré e pós-tratamento ortodôntico corretivo de 40 pacientes, divididos em dois grupos. O grupo 1 constou de 20 pacientes (13 femininos e 7 masculinos que se submeteram a tratamento ortodôntico com extrações de primeiros pré-molares. O grupo 2 foi constituído por 20 pacientes (13 femininos e 7 masculinos tratados ortodonticamente sem extrações. Foram estabelecidas medidas angulares, com o objetivo de avaliar as inclinações e medidas lineares, para analisar as modificações no sentido vertical dos terceiros molares. Todos os dados foram mensurados duas vezes, cujos valores médios foram submetidos dos testes t emparelhado e teste t independente. RESULTADOS: os resultados mostraram diferenças estatisticamente significantes (pAIM: The aim of this study is to evaluate the positional changes occurred with lower third molars in patients treated orthodontically with extractions of first premolars. METHODS: A total of 80 panoramic radiographs obtained at pre and post-treatment periods from 40 patients, were divided into 2 groups: group 1 were composed by 20 patients (13 female and 7 male that were submitted to orthodontic treatment with extraction of first premolars; group 2 were composed by 20 patients (13 female and 7 male that were submitted to orthodontic treatment without extractions. Angular measurements were established to evaluate the tipping and linear measurements were established to analyze the vertical changes of the lower third molars. All data were measured twice and averaged, then submitted to paired and unpaired test. RESULTS: Significant differences were found between the initial and final angular measurements of group 1 (p<0.01. CONCLUSIONS: It was concluded that

  12. Clinical evaluation of piroxicam-FDDF and azithromycin in the prevention of complications associated with impacted lower third molar extraction.

    Science.gov (United States)

    Graziani, F; Corsi, L; Fornai, M; Antonioli, L; Tonelli, M; Cei, S; Colucci, R; Blandizzi, C; Gabriele, M; Del Tacca, M

    2005-12-01

    Combined treatments with non-steroidal anti-inflammatory drugs and antibiotics may offer significant benefits in the prevention of pain and infections associated with oral surgery. In this study, piroxicam and azithromycin were administered to patients undergoing dental extraction to examine the efficacy of piroxicam in the prevention of post-operative pain and inflammatory complications, either in the absence or in the presence of a concomitant antibiotic treatment. Thirty patients were randomly assigned to three groups and treated for 3 days, before impacted lower third molar removal, as follows: (1) sublingual piroxicam-FDDF (fast dissolving dosage formulation) 20 mg/day; (2) oral azithromycin 500 mg/day; (3) piroxicam-FDDF 20 mg/day plus azithromycin 500 mg/day. Oral acetaminophen (500 mg tablets) was allowed as rescue analgesic medication. Pain intensity was evaluated on a 100-mm visual-analogue scale after dental extraction (day 1), and at days 2, 3, 7 after surgery. Edema and trismus were estimated at days 2 and 7. At days 1 and 2, pain intensity was significantly lower in patients treated with piroxicam-FDDF, either alone (p operative period, piroxicam-FDDF effectively counteracts post-surgical pain and inflammatory reactions in oral tissues. Upon combined treatment with piroxicam-FDDF and azithromycin, the macrolide antibiotic may reduce the influence of piroxicam on post-operative inflammation, without affecting its beneficial effect on surgical pain.

  13. Pulp tissue response to Portland cement associated with different radio pacifying agents on pulpotomy of human primary molars.

    Science.gov (United States)

    Marques, N; Lourenço Neto, N; Fernandes, A P; Rodini, C; Hungaro Duarte, M; Rios, D; Machado, M A; Oliveira, T

    2015-12-01

    The objective of this research was to evaluate the response of Portland cement associated with different radio pacifying agents on pulp treatment of human primary teeth by clinical and radiographic exams and microscopic analysis. Thirty mandibular primary molars were randomly divided into the following groups: Group I - Portland cement; Group II - Portland cement with iodoform (Portland cement + CHI3 ); Group III - Portland cement with zirconium oxide (Portland cement + ZrO2 ); and treated by pulpotomy technique (removal of a portion of the pulp aiming to maintain the vitally of the remaining radicular pulp tissue using a therapeutic dressing). Clinical and radiographic evaluations were recorded at 6, 12 and 24 months follow-up. The teeth at the regular exfoliation period were extracted and processed for histological analysis. Data were tested using statistical analysis with a significance level of 5%. The microscopic findings were descriptively analysed. All treated teeth were clinically and radiographically successful at follow-up appointments. The microscopic analysis revealed positive response to pulp repair with hard tissue barrier formation and pulp calcification in the remaining roots of all available teeth. The findings of this study suggest that primary teeth pulp tissue exhibited satisfactory biological response to Portland cement associated with radio pacifying agents. However, further studies with long-term follow-up are needed to determine the safe clinical indication of this alternative material for pulp therapy of primary teeth.

  14. Una rara complicación en la extracción del tercer molar inferior incluido: Caso clínico A rare complication during the extraction of the included inferior third molar: Case report

    Directory of Open Access Journals (Sweden)

    S. Aboul-Hosn Centenero

    2009-10-01

    Full Text Available Presentamos una rara complicación ocurrida tras el intento de extracción del tercer molar incluido en una consulta odontológica bajo anestesia local. El paciente requirió ingreso en el servicio de urgencias para la extracción, de forma urgente, de la pieza a través de un abordaje cervical bajo anestesia general e intubación nasaotraqueal por desplazamiento de la pieza hacia la fosa submandibular durante el intento de extracción ambulatoria. El paciente presentó signos evidentes de inflamación en la región submandibular con desplazamiento de la vía aérea hacia el lado opuesto. Las complicaciones derivadas de la cirugía del tercer molar son, en algunos casos, mortales y pueden aparecer tras un acto quirúrgico aparentemente de bajo riesgo. Estas complicaciones han sido ampliamente descritas en la literatura. Aún así consideramos importante el presentar complicaciones poco frecuentes en la cirugía del tercer molar ya que esto nos permitirá conocerlas y ofrecer la mejor solución en cada caso.We present a rare complication that appeared after an attempt to extract the included third molar with local anaesthesia at the odontologist office. The patient was admitted to the emergency room to have the tooth removed cervically under general anaesthesia and nasotracheal tubation because of its movement towards the submandibular fossa during the ambulatory extraction attempt. The patient showed evident signs of swelling in submandibular region and the airway was displaced towards the opposite side. The resulting complications from the third molar surgery are, in some cases, mortal and can occur after an apparently low risk surgery. These complications have been thoroughly described in literature. Still, we consider it important to describe infrequent complications of third molar surgery because it will allow us to know more about them and offer the best solution in every case.

  15. An in vitro analysis of separation of multi-use ProTaper Universal and ProTaper Next instruments in extracted mandibular molar teeth.

    Science.gov (United States)

    Ertas, Huseyin; Capar, Ismail Davut

    2015-01-01

    This study investigated the separation incidence of reused ProTaper Universal and ProTaper Next rotary instruments and identified the location of separated fragments. The root canals of extracted human mandibular molars were prepared with 10 assorted sets of ProTaper Universal and ProTaper Next instruments. After each preparation, instrument sets were autoclaved. This arrangement was repeated until an instrument fractured. The number of prepared teeth until fracture occurred was recorded for each instrument set. Teeth in which the instruments fractured were analyzed to determine the separation grade, apical relation, and coronal position. Fracture surfaces of the instruments were examined with scanning electron microscope. ProTaper Universal instruments fractured after application to a mean of 7.3 teeth, and ProTaper Next instruments after application to a mean of 5.7 teeth (p > 0.05). In the ProTaper Universal and ProTaper Next groups, F2 and X1 were the most commonly fractured instruments, respectively. Torsional and cyclic failures were evenly distributed in both the groups. The mean lengths of the fractured fragments of the instruments showed no statistically significant difference. The distance between the tip of the fractured instruments and apical constriction was similar (p > 0.05). However, the mean distance between the root canal orifice and coronal part of the fractured instrument was shorter in the ProTaper Next group (p ProTaper Universal and ProTaper Next instruments was the same for preparation of mandibular molar teeth. None of the instruments were fractured in the first usage.

  16. Molar Pregnancy

    Science.gov (United States)

    ... may check for other medical problems, including: Preeclampsia Hyperthyroidism Anemia A molar pregnancy can't continue as a normal viable pregnancy. To prevent complications, the molar tissue must be removed. Treatment usually consists of one or more of the ...

  17. MOLAR UPRIGHTING

    Directory of Open Access Journals (Sweden)

    Eka Erwansyah

    2006-04-01

    Full Text Available The mesial tipping of molar is frequently found in orthodontic cases. This molar malposition must be corrected since it may cause periodontal disorders, occlusal interferences, and temporomandibular joint dysfunction, and is often needed in planning a fixed bridge. This paper is a literature study to discuss about appliance designs, indication, and contraindications, and complication and treatment protocols of molar uprighting by fixed orthodontic appliances. By knowing the techniques of molar uprighting, the moments mentioned above can be avoided.

  18. Fluorescent probes sensitive to changes in the cholesterol-to-phospholipids molar ratio in human platelet membranes during atherosclerosis

    Science.gov (United States)

    Posokhov, Yevgen

    2016-09-01

    Environment-sensitive fluorescent probes were used for the spectroscopic visualization of pathological changes in human platelet membranes during cerebral atherosclerosis. It has been estimated that the ratiometric probes 2-(2‧-hydroxyphenyl)-5-phenyl-1,3,4-oxadiazole and 2-phenyl-phenanthr[9,10]oxazole can detect changes in the cholesterol-to-phospholipids molar ratio in human platelet membranes during the disease.

  19. Lingual split versus surgical bur technique in the extraction of impacted mandibular third molars: a systematic review.

    Science.gov (United States)

    Steel, Ben

    2012-09-01

    The relative merits of different surgical techniques to extract impacted mandibular third molars have been debated for many years. A simple classification is those using a bur and those using a chisel. This article seeks to identify any differences in postoperative outcomes between the surgical bur and lingual split techniques. Using inclusion criteria allowing randomized controlled trials only, 5 studies are identified of which 4 are used in the analysis. The following outcomes are investigated: pain, swelling, trismus, bleeding, delayed healing/infection, and disturbance to lingual and inferior alveolar nerve function. The limited analysis allowed by the number and size of the studies leads to tentative conclusions of no difference between postoperative pain and swelling, and some evidence of less trismus for the lingual split technique. There is some weak evidence of a similar incidence of neurological sequelae between the 2 techniques, but this is not a strong conclusion owing to the small size of the included studies. There are inadequate data regarding bleeding and delayed healing/infection for analysis. Copyright © 2012 Elsevier Inc. All rights reserved.

  20. Cervical and crown outline analysis of worn Neanderthal and modern human lower second deciduous molars.

    Science.gov (United States)

    Benazzi, Stefano; Fornai, Cinzia; Buti, Laura; Toussaint, Michel; Mallegni, Francesco; Ricci, Stefano; Gruppioni, Giorgio; Weber, Gerhard W; Condemi, Silvana; Ronchitelli, Annamaria

    2012-12-01

    Despite the general increase in digital techniques for dental morphometric analyses, only a few methods are available to study worn teeth. Moreover, permanent dentitions are studied much more frequently than deciduous teeth. In this study, we address both issues by providing a taxonomic classification of Neanderthal and modern human (MH) lower second deciduous molars (dm(2) s) through the analysis of crown and cervical outlines. Crown and cervical outlines were obtained from a three-dimensional (3D) digital sample of uniformly oriented dm(2) s. Both outlines were centered on the centroid of their area and represented by 16 pseudolandmarks obtained by equiangularly spaced radial vectors out of the centroid. We removed size information from the oriented and centered outlines with a uniform scaling of the pseudolandmark configurations to unit Centroid Size. Group shape variation was evaluated separately for the dm(2) crown and cervical outlines through a shape-space principal component (PC) analysis. Finally, quadratic discriminant analysis of a subset of PCs was used to classify the specimens. Our results demonstrate that both outlines successfully separate the two groups. Neanderthals showed a buccodistal expansion and convex lingual outline shape, whilst MHs have buccodistal reduction and straight lingual outline shape. Therefore, we confirmed that the cervical outline represents an effective parameter for distinguishing between the two taxa when dealing with worn or damaged dm(2) s.

  1. Effects of Nd:YAG laser pulpotomy on human primary molars.

    Science.gov (United States)

    Liu, Jeng-fen

    2006-05-01

    The purpose of this study was to compare the effects of Nd:YAG laser pulpotomy to formocresol pulpotomy on human primary teeth. Patients with a primary tooth that required pulpotomy because of pulpal exposure to caries, were selected for this study. After removal of coronal pulpal tissue, Nd:YAG laser at 2 W, 20 Hz, 100 mJ or a 1:5 dilution of formocresol was introduced into the canal orifice for complete hemostasis. IRM paste was then placed over the pulp stump, and the tooth was restored either with composite resin or stainless steel crown. Sixty-eight teeth were treated with Nd:YAG laser and followed up for 6 to 64 months. Clinical success was achieved in 66 out of the 68 teeth (97 %), and 94.1 % were radiographically successful. In the control group, 69 primary molars were treated with formocresol and followed up for 9 to 66 months; 85.5 and 78.3% achieved clinical and radiographic success, respectively. The success rate of Nd:YAG laser pulpotomy was significantly higher than that of formocresol pulpotomy. The permanent successors of the laser-treated teeth erupted without any complications.

  2. Immunohistochemical Analysis of Human Homologue of Drosophila Patched (PTCH) in Dental Follicles of Impacted Third Molars

    OpenAIRE

    de OLIVEIRA, David Moraes; Ferreira da Silveira, Marcia Maria; de Souza Andrade, Emanuel Savio; Veras Sobral, Ana Paula; Saquete Martins-Filho, Paulo Ricardo; SANTOS, Thiago de Santana; Amorim de Oliveira, Patricia Leimig; Peixoto, Aline Carvalho; Santana de Souza Santos, Jadson Alipio; PIVA, MARTA RABELLO

    2012-01-01

    This study investigated the immunodetection of PTCH in epithelial components of dental follicles associated with impacted third molars without radiographic signs of pathosis. One hundred and five specimens of dental follicles associated with impacted third molars with incomplete rhizogenesis (between Nolla's stage 6 and 9) were surgically removed from 56 patients. Epithelial cell proliferation was determined by using immunohistochemical labeling. Statistical analysis was performed using Fishe...

  3. 下颌阻生智齿拔除仿头模模型的建立%Establishment of a simulation model for mandibular third molar extraction

    Institute of Scientific and Technical Information of China (English)

    陈慧敏; 江泳; 许桐楷; 王恩博

    2016-01-01

    目的:使用口腔常用材料制作可反复使用的下颌阻生智齿拔除仿头模模型,并将其应用于临床前教学。方法:根据口腔常用材料特点、下颌阻生智齿的解剖特点及拔除的技术特点,设计并制作可反复使用的下颌阻生智齿拔除模型;将该模型固定于仿头模内,由口腔颌面外科临床医师及实习学生试用,采用问卷调查的方法对使用效果进行评估。结果:成功设计并制作出了下颌阻生智齿拔除模型;问卷调查结果显示该仿头模模型很好的模拟了临床下颌阻生智齿的拔除。结论:使用口腔常用材料可以成功的制作出下颌阻生智齿拔除模型,有助于临床实际操作。%Objective:To establish a model of mandibular impact third molar extraction with common dental materials for pre-clinical trai-ning.Methods:Based on the characteristics of the common dental materials,the anatomy and extraction skill of mandibular impact third molar,the dental model for mandibular impact third molar extraction was designed and made.Then,this dental model was placed in the head-simulation model as required,and used by the dentists and the students.The questionnaire was designed and used to evalu-ate the effects of the model.Results:A new method of designing and making a dental model for mandibular impact third molar extraction with the common dental materials was established successfully.The questionnaire results showed that all the dentists and students agreed that this model could simulate the mandibular third molar extraction procedure.Conclusion:Simulation model of the mandibular third molar extraction can help students for the following clinical practice.

  4. False-negative urine human chorionic gonadotropin in molar pregnancy: " The high-dose hook effect" !

    Directory of Open Access Journals (Sweden)

    Sujata Narendra Datti

    2015-01-01

    Full Text Available Failure to detect pregnancy in the emergency situations can have important consequences. These include missing of ectopic pregnancy (the leading cause of first-trimester pregnancy-related maternal death, administration of medications contraindicated in pregnancy, fetal radiation exposure, and medico legal problems. This in turn has led to the dictum to check for pregnancy in all women of child-bearing age group. Urine pregnancy (human chorionic gonadotropin [hCG] test is the commonly used test to rule out pregnancy and has been reported by Griffey et al. in their study to achieve 100% sensitivity and 99.2% specificity in a clinical setting, resulting in a positive predictive value of 98.3% and a negative predictive value of nearly 100%. However, the sensitivity is influenced not only by the quantity of β hCG but on its variants that vary with different weeks of pregnancy. β hCG is present in several variant forms that change in their concentrations at different stages of pregnancy. In spite of its high sensitivity, in the presence of molar pregnancy that is associated with very high levels of β hCG it fails to detect the antigen (β hCG. This is explained by the phenomenon known as "high-dose hook effect" which further leads to delay in diagnosis and treatment. This can be overcome by dilution of the sample. In such cases, diagnosis will be made by serum β hCG and ultrasound (USG. Here, we present a case of gravida 2 para 1 living 1 with 2΍ months amenorrhea with bleeding p/v and pain abdomen of 20 days duration whose urine β hCG was repeatedly negative and diagnosis was made by serum β hCG and USG.

  5. Using occlusal wear information and finite element analysis to investigate stress distributions in human molars.

    Science.gov (United States)

    Benazzi, Stefano; Kullmer, Ottmar; Grosse, Ian R; Weber, Gerhard W

    2011-09-01

    Simulations based on finite element analysis (FEA) have attracted increasing interest in dentistry and dental anthropology for evaluating the stress and strain distribution in teeth under occlusal loading conditions. Nonetheless, FEA is usually applied without considering changes in contacts between antagonistic teeth during the occlusal power stroke. In this contribution we show how occlusal information can be used to investigate the stress distribution with 3D FEA in lower first molars (M(1)). The antagonistic crowns M(1) and P(2)-M(1) of two dried modern human skulls were scanned by μCT in maximum intercuspation (centric occlusion) contact. A virtual analysis of the occlusal power stroke between M(1) and P(2)-M(1) was carried out in the Occlusal Fingerprint Analyser (OFA) software, and the occlusal trajectory path was recorded, while contact areas per time-step were visualized and quantified. Stress distribution of the M(1) in selected occlusal stages were analyzed in strand7, considering occlusal information taken from OFA results for individual loading direction and loading area. Our FEA results show that the stress pattern changes considerably during the power stroke, suggesting that wear facets have a crucial influence on the distribution of stress on the whole tooth. Grooves and fissures on the occlusal surface are seen as critical locations, as tensile stresses are concentrated at these features. Properly accounting for the power stroke kinematics of occluding teeth results in quite different results (less tensile stresses in the crown) than usual loading scenarios based on parallel forces to the long axis of the tooth. This leads to the conclusion that functional studies considering kinematics of teeth are important to understand biomechanics and interpret morphological adaptation of teeth.

  6. The effects of a topical gel containing chitosan, 0,2% chlorhexidine, allantoin and despanthenol on the wound healing process subsequent to impacted lower third molar extraction

    OpenAIRE

    Madrazo Jiménez, Marta; Rodríguez Caballero, Ángela; Serrera Figallo, María Angeles; Garrido Serrano, Roberto; Gutiérrez Corrales, Aida; Gutiérrez Pérez, José Luis; Torres-Lagares, Daniel

    2016-01-01

    Background Despite efforts to prevent postoperative discomfort, there are still many immediate side effects associated with the surgical extraction of impacted lower third molars. Cicatrization is a physiological process through which the loss of integrity of oral mucosa is recovered and damaged tissues are repaired. Bexident Post (ISDIN, Spain) is a topical gel that contains chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol. While this gel has many clinical indications, there are no p...

  7. Effect of oral contraceptive use on the incidence of dry socket in females following impacted mandibular third molar extraction: a meta-analysis.

    Science.gov (United States)

    Xu, J-L; Sun, L; Liu, C; Sun, Z-H; Min, X; Xia, R

    2015-09-01

    The aim of this comprehensive meta-analysis was to provide evidence-based data to test whether oral contraceptive (OC) use can promote the incidence of dry socket (DS) in females following impacted mandibular third molar extraction. PubMed, the Cochrane Library, and Elsevier Science Direct databases were searched. The pooled risk ratio (RR) with 95% confidence interval (CI) was calculated using fixed-effects or random-effects model analysis. Heterogeneity among studies was evaluated with the Cochran test and I(2) statistic. Study quality was assessed with the Newcastle-Ottawa scale. Of 70 articles identified in the search, 12 reporting 16 clinical controlled trials were included in this study. The incidence of DS was significantly greater in the OC groups than in the control groups (RR 1.80, 95% CI 1.33-2.43). Subgroup analyses showed that the unit assessed (tooth or patient), the region in which the study was conducted, and the intervention were not related to the incidence of DS in females taking OC after impacted mandibular third molar extraction. The sensitivity analysis showed no significant change when any one study was excluded. Publication bias was also not detected. This study suggests that OC use may promote the incidence of DS in females following impacted mandibular third molar extraction.

  8. Clinical Analysis of Orthodontic Treatmeat with First Molar Extraction%拔除第一磨牙的正畸治疗临床分析

    Institute of Scientific and Technical Information of China (English)

    胡林华; 黎秀萍; 黄纯; 林晓瑜; 邱翠旋

    2011-01-01

    Objective To determine the normal principle of treatment plans, appropriate methods of space closure and clinical effects in orthodontic treatment cases with first molar extractions. Methods Forty-two cases with first molar extractions in the past five year were included. The cases with normal premolar extractions served as control group. All cases were treated with MBT. The treatment time and clinical effects were compared. Results The average treatment time of first molar extractions was ( 22 ± 5.6 ) months, the control group was ( 20 ± 8.9 ) months. There was no significant difference between two groups. All the cases were finished with paralleling root, satisfying molar relationship, normal overbite and overjet. Conclusion With the development of orthodontic technique, the same clinical effects can be achieved in the cases with first molar extractions and premolar extractions.%目的 探讨正畸拔除第一磨牙矫治的临床疗效.方法 试验组42例拔除第一恒磨牙(含既拔除磨牙,也拔除前磨牙)后进行正畸治疗;对照组42例常规拔除前磨牙后进行正畸治疗.所有患者均采用直丝弓矫治器矫治,比较2组的疗程和疗效.结果 试验组平均疗程为(22±5.6)个月,对照组平均疗程为(20±8.9)个月, 2组疗程差异无统计学意义(t=1.232,P>0.05).所有患者均取得较好疗效,牙根平行,磨牙达尖窝关系,前牙覆牙合、覆盖正常,软组织侧貌形态良好.结论 拔除第一磨牙进行矫治,在精心设计、合理应用支抗的情况下,可取得理想的矫治效果.

  9. Effect of the local administration of betamethasone on pain, swelling and trismus after impacted lower third molar extraction. A randomized, triple blinded, controlled trial

    Science.gov (United States)

    Marques, José; Pié-Sánchez, Jordi; Valmaseda-Castellón, Eduard; Gay-Escoda, Cosme

    2014-01-01

    Objectives: The aim of this study is to compare the analgesic and anti-inflammatory effects of the local postoperative administration of a single 12-mg dose of betamethasone after the surgical removal of impacted lower third molars. Study Design: A split-mouth, triple-blind, randomized, placebo-controlled clinical trial of 25 patients requiring the surgical removal of symmetrical lower third molars was performed. In the experimental side, a 12-mg dose of betamethasone was administered submucosally after the surgical procedure, while in the control side a placebo (sterile saline solution) was injected in the same area. To assess postoperative pain, visual analogue scales and the consumption of rescue analgesic were used. The facial swelling and trismus were evaluated by measuring facial reference distances and maximum mouth opening. Results: There were no significant differences between the two study groups regarding postoperative pain, facial swelling and trismus. Conclusions: The injection of a single dose of betamethasone does not seem to reduce pain, facial swelling and trismus after impacted lower third molar removal when compared to placebo. Key words:Third molar extraction, corticosteroids, betamethasone. PMID:24121915

  10. ["Molar-incisor hypomineralization"].

    Science.gov (United States)

    Kellerhoff, Nadja-Marina; Lussi, Adrian

    2004-01-01

    Hypocalcification of the enamel is the most common developmental disorder observed in teeth. The prevalence of this kind of hypomineralisation is about 10-19%. These molars are often referred to as cheese molars, because the lesions clinically resemble cheese in color and consistency. Other descriptions are: idiopathic enamel hypomineralisation in the permanent first molars, idiopathic enamel opacities in the permanent first molars, non fluoride enamel hypomineralisation in the permanent first molars, non-endemic mottling of enamel in the permanent first molars. Molar-Incisor Hypomineralisation is today the proposed expression for this disease. Occlusal surfaces of the first permanent molar are most commonly affected. The lesions are more frequent in the upper jaw than in the lower jaw. The incisors are affected to a lesser degree than the molars. Several aetiological factors can cause these defects. Some studies show a relation between intake of dioxins via mother's milk after prolonged breast feeding and developmental defects of the child's teeth. Because the ameloblasts are very sensitive to oxygen supply, complications involving oxygen shortages during birth or respiratory diseases such as asthma or bronchitis and pneumonia are discussed as further aetiological factors. Renal insufficiency, hypoparothyroidism, diarrhoea, malabsorption and malnutrition and high-fever diseases can be other reasons for the occurrence of these defects. Defective enamel can be a locus of lowered resistance for caries. Histologically there are areas of porosity of varying degrees. The affected teeth can be very sensitive to air, cold, warm and mechanical stimuli. Toothbrushing may create toothache in these teeth. We therefore suggest that these patients receive intensified prevention with fluoride varnish, a fissure sealing, GIZ, composits, stainless steel crowns or implants. In some cases an interdisciplinary approach with an orthodontist can result in the extraction of the molars

  11. Brief communication: two human fossil deciduous molars from the Sangiran dome (Java, Indonesia): outer and inner morphology.

    Science.gov (United States)

    Zanolli, Clément; Bondioli, Luca; Mancini, Lucia; Mazurier, Arnaud; Widianto, Harry; Macchiarelli, Roberto

    2012-03-01

    Currently, the human deciduous dental record from the Pleistocene deposits of the Sangiran Dome, Java, consists of only eight specimens. Here we report two deciduous crowns collected near the village of Pucung. While their precise geo-chronological context remains unknown, a provenance from the Early-Middle Pleistocene Kabuh Formation, or from the Early Pleistocene "Grenzbank Zone," is very likely. These isolated specimens consist of an upper first molar (PCG.1) and a lower second molar (PCG.2). Taxonomic discrimination of the Indonesian tooth record is difficult because of the convergence in crown size and appearance between Pongo and Homo. Accordingly, as PCG.2 still bears a concretion masking most of its features, we coupled the outer analysis of the two specimens with an investigation of their inner morphology. In addition to external characteristics, virtual imaging and quantitative assessment of inner morphology and tissue proportions support an attribution to the taxon Homo, and we preliminary allocate both specimens toH. erectus.

  12. The horizontal inclination angle is associated with the risk of inferior alveolar nerve injury during the extraction of mandibular third molars.

    Science.gov (United States)

    Ishii, S; Abe, S; Moro, A; Yokomizo, N; Kobayashi, Y

    2017-07-29

    The extraction of mandibular third molars can lead to injury to the inferior alveolar nerve. Hence, it is important to assess the proximity of the root to the inferior alveolar canal before extraction. The classification system of Pell and Gregory and the Winter classification are commonly used to evaluate the positional relationship of the third molar based on radiographs. This retrospective study involving 105 mandibular third molars was performed to assess whether these systems reflect the proximity of the root to the canal (based on computed tomography images), and to identify risk factors for nerve injury. Regarding the prediction of computed tomography-verified canal invasion, the sensitivity, specificity, and positive and negative predictive values were high for each Pell and Gregory category when there was radiographic evidence. The mean distance of invasion was significantly greater in class III than in class I. However, there were no significant differences between the Winter inclination categories. The mean distance differed significantly between a horizontal inclination angle to the buccal side of >5° and an angle of ≤5°. Thus, a horizontal inclination angle >5° represents a novel risk factor for nerve injury. Copyright © 2017 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  13. Efficacy of amoxicillin and amoxicillin/clavulanic acid in the prevention of infection and dry socket after third molar extraction. A systematic review and meta-analysis

    Science.gov (United States)

    Arteagoitia, María-Iciar; Barbier, Luis; Santamaría, Gorka; Ramos, Eva

    2016-01-01

    Background Prophylactic use of amoxicillin and amoxicillin/clavulanic acid, although controversial, is common in routine clinical practice in third molar surgery. Material and Methods Our objective was to assess the efficacy of prophylactic amoxicillin with or without clavulanic acid in reducing the incidence of dry socket and/or infection after third molar extraction. We conducted a systematic review and meta-analysis consulting electronic databases and references in retrieved articles. We included double-blind placebo-controlled randomized clinical trials published up to June 2015 investigating the efficacy of amoxicillin with or without clavulanic acid on the incidence of the aforementioned conditions after third molar extraction. Relative risks (RRs) were estimated with a generic inverse-variance approach and a random effect model using Stata/IC 13 and Review Manager Version 5.2. Stratified analysis was performed by antibiotic type. Results We included 10 papers in the qualitative review and in the quantitative synthesis (1997 extractions: 1072 in experimental groups and 925 in controls, with 27 and 74 events of dry socket and/or infection, respectively). The overall RR was 0.350 (p< 0.001; 95% CI 0.214 to 0.574). We found no evidence of heterogeneity (I2=0%, p=0.470). The number needed to treat was 18 (95% CI 13 to 29). Five studies reported adverse reactions (RR=1.188, 95% CI 0.658 to 2.146, p =0.567). The RRs were 0.563 for amoxicillin (95% CI 0.295 to 1.08, p=0.082) and 0.215 for amoxicillin/clavulanic acid (95% CI 0.117 to 0.395, p<0.001). Conclusions Prophylactic use of amoxicillin does not significantly reduce the risk of infection and/or dry socket after third molar extraction. With amoxicillin/clavulanic acid, the risk decreases significantly. Nevertheless, considering the number needed to treat, low prevalence of infection, potential adverse reactions to antibiotics and lack of serious complications in placebo groups, the routine prescription of

  14. Comparison of buccal infiltration of 4% articaine with 1 : 100,000 and 1 : 200,000 epinephrine for extraction of maxillary third molars with pericoronitis: a pilot study

    National Research Council Canada - National Science Library

    Lima, Jr, José Lacet; Dias-Ribeiro, Eduardo; Ferreira-Rocha, Julierme; Soares, Ramon; Costa, Fábio Wildson Gurgel; Fan, Song; Sant'ana, Eduardo

    2013-01-01

    We compared the buccal infiltration of 4% articaine with 1 : 100,000 or 1 : 200,000 epinephrine without a palatal injection for the extraction of impacted maxillary third molars with chronic pericoronitis...

  15. Light-cured calcium hydroxide vs formocresol in human primary molar pulpotomies: a randomized controlled trial.

    Science.gov (United States)

    Zurn, Derek; Seale, N Sue

    2008-01-01

    The purpose of this prospective study was to compare light-cured calcium hydroxide (Ca(OH)2) with diluted formocresol (FC) for its success as a primary molar pulpotomy medicament Selection criteria included at least 2 matching, asymptomatic, contralateral primary molars requiring vital pulpotomies. Matched teeth in each patient were randomized to receive either Ca(OH)2 or FC as a pulpotomy medicament. All teeth were restored with prefabricated metal crowns. Twenty patients (34 pairs of teeth) were followed clinically and radiographically for > or =1 year. Two blinded, standardized, and calibrated examiners evaluated and scored each radiograph for signs of pathology, based upon a modified scale previously proposed. Findings were grouped in: (a) 0 - 6; (b) 7 - 12; and (c) 13 - 24 month intervals. Radiographic scoring favored the FC group of the 7- to 12- and 13- to 24-month intervals (Pformocresol as a pulpotomy agent.

  16. Hawthorn extract inhibits human isolated neutrophil functions.

    Science.gov (United States)

    Dalli, Ernesto; Milara, Javier; Cortijo, Julio; Morcillo, Esteban J; Cosín-Sales, Juan; Sotillo, José Francisco

    2008-06-01

    Hawthorn extract is a popular herbal medicine given as adjunctive treatment for chronic heart failure. In contrast to the cardiac properties of hawthorn extract, its anti-inflammatory effect has been scarcely investigated. This study examines the effects of a dry extract of leaves and flowers of Crataegus laevigata on various functional outputs of human neutrophils in vitro. Incubation of human neutrophils obtained from peripheral blood of healthy donors with C. laevigata extract (0.75-250 microg/ml) inhibited N-formyl-Met-Leu-Phe (FMLP)-induced superoxide anion generation, elastase release and chemotactic migration with potency values of 43.6, 21.9, and 31.6 microg/ml, respectively. By contrast, serum-opsonized zymosan-induced phagocytosis was unaltered by plant extract. C. laevigata extract (125 microg/ml) reduced FMLP-induced leukotriene B(4) production and lipopolysaccharide-induced generation of tumour necrosis factor-alpha and interleukin-8. Extract inhibited FMLP-induced intracellular calcium signal with potency of 17.4 microg/ml. Extract also markedly inhibited the extracellular calcium entry into calcium-depleted neutrophils, and the thapsigargin-induced intracellular calcium response. In conclusion, C. laevigata extract inhibited various functional outputs of activated human neutrophils which may be relevant to the pathophysiology of cardiac failure.

  17. How will mandibular third molar surgery affect mandibular second molar periodontal parameters?

    Directory of Open Access Journals (Sweden)

    Reza Tabrizi

    2013-01-01

    Conclusion: Unlike plenty of researches that have shown improvement of periodontal parameters of the second molar after extraction of impacted third molar, our study showed a significant increase in PD at the distal aspect of the second molar. Further follow-up on clinical and radiological parameters are required for more profound understanding of the long-term effects of third molar extraction on the periodontal parameters of the adjacent second molar.

  18. The effects of a topical gel containing chitosan, 0,2% chlorhexidine, allantoin and despanthenol on the wound healing process subsequent to impacted lower third molar extraction

    Science.gov (United States)

    Madrazo-Jiménez, Marta; Rodríguez-Caballero, Ángela; Serrera-Figallo, María-Ángeles; Garrido-Serrano, Roberto; Gutiérrez-Corrales, Aida; Gutiérrez-Pérez, José-Luis

    2016-01-01

    Background Despite efforts to prevent postoperative discomfort, there are still many immediate side effects associated with the surgical extraction of impacted lower third molars. Cicatrization is a physiological process through which the loss of integrity of oral mucosa is recovered and damaged tissues are repaired. Bexident Post (ISDIN, Spain) is a topical gel that contains chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol. While this gel has many clinical indications, there are no published clinical trials evaluating its use in impacted mandibular third molar surgery. This study aims to clinically evaluate the efficacy of a gel containing chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol on wound healing and reduction of postoperative side effects and complications after extraction of an impacted mandibular third molar. Material and Methods A split-mouth design study was carried out on a total of 50 bilaterally and symmetrically impacted third molar extractions, which were randomly placed into either a control group (CG=25) or an experimental group (EG=25). Patients were all informed of the purpose of the study and provided written consent. All procedures were carried out by the same dental practitioner, in accordance with standard surgical protocol. A different dental practitioner, unaware of which treatment had been applied, provided follow-up care. The EG applied 10 ml of topical gel composed of chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol to the surgical wound three times a day for 10 days, patients in the CG did not apply any gel. Results The groups were homogeneous insofar as potentially confounding variables. No significant findings were found regarding postoperative swelling and pain. Neither of the groups displayed poor healing or infectious complications of the wound during the postoperative period. In all the recorded follow-ups (Day 7 p=0.001, and Day 14 p=0.01), the wound’s aesthetic appearance was better in the EG

  19. Third molar infections.

    Science.gov (United States)

    Gutiérrez-Pérez, José Luis

    2004-01-01

    Pericoronitis is an infectious disease often associated with the eruption of a third molar. It can be either acute (serous and suppurative) or chronic. Pain is usually the predominant symptom in acute stages, whereas chronic forms of the disease may display very few symptoms. Both present exudate. The infection is multimicrobial, predominantly caused strictly by betalactamase-producing anaerobeic microorganisms. Treatment measures are symptomatic, antimicrobial and surgical. Antimicrobial treatment is indicated for preoperative prophylaxis when there is a high risk of postoperative infection and, during the acute stages of suppurative pericoronitis when surgery must be postponed. First-line treatment in this case consists of amoxicillin with associated clavulanic acid. Although surgical treatment of pericoronitis presenting at the third molar is indicated as a Grade C recommendation for extraction, it is the most common indication for extraction of a retained third molar, owing to the improved quality of life it can offer the patient.

  20. Efficacy of amoxicillin and amoxicillin/clavulanic acid in the prevention of infection and dry socket after third molar extraction. A systematic review and meta-analysis.

    Science.gov (United States)

    Arteagoitia, M-I; Barbier, L; Santamaría, J; Santamaría, G; Ramos, E

    2016-07-01

    Prophylactic use of amoxicillin and amoxicillin/clavulanic acid, although controversial, is common in routine clinical practice in third molar surgery. Our objective was to assess the efficacy of prophylactic amoxicillin with or without clavulanic acid in reducing the incidence of dry socket and/or infection after third molar extraction. We conducted a systematic review and meta-analysis consulting electronic databases and references in retrieved articles. We included double-blind placebo-controlled randomized clinical trials published up to June 2015 investigating the efficacy of amoxicillin with or without clavulanic acid on the incidence of the aforementioned conditions after third molar extraction. Relative risks (RRs) were estimated with a generic inverse-variance approach and a random effect model using Stata/IC 13 and Review Manager Version 5.2. Stratified analysis was performed by antibiotic type. We included 10 papers in the qualitative review and in the quantitative synthesis (1997 extractions: 1072 in experimental groups and 925 in controls, with 27 and 74 events of dry socket and/or infection, respectively). The overall RR was 0.350 (p<0.001; 95% CI 0.214 to 0.574). We found no evidence of heterogeneity (I2=0%, p=0.470). The number needed to treat was 18 (95% CI 13 to 29). Five studies reported adverse reactions (RR=1.188, 95% CI 0.658 to 2.146, p =0.567). The RRs were 0.563 for amoxicillin (95% CI 0.295 to 1.08, p=0.082) and 0.215 for amoxicillin/clavulanic acid (95% CI 0.117 to 0.395, p<0.001). Prophylactic use of amoxicillin does not significantly reduce the risk of infection and/or dry socket after third molar extraction. With amoxicillin/clavulanic acid, the risk decreases significantly. Nevertheless, considering the number needed to treat, low prevalence of infection, potential adverse reactions to antibiotics and lack of serious complications in placebo groups, the routine prescription of amoxicillin with or without clavulanic acid is not

  1. A clinical evaluation of a bioresorbable membrane and porous hydroxyapatite in the treatment of human molar class II furcations

    Directory of Open Access Journals (Sweden)

    K Gita Malathi

    2013-01-01

    Full Text Available Background: The ultimate goal of periodontal therapy is predictable regeneration of a functional attachment apparatus destroyed as a result of periodontitis. Reconstructive procedures have been used with varying success during the past decades to accomplish this goal. Aim: To evaluate whether the use of porous hydroxyapatite alone or a bioresorbable membrane alone would enhance the clinical results in the treatment of class II furcation defects in human lower molars. Materials and Methods: Fifteen patients with chronic periodontitis, aged between 39 and 49 years, with a pair of similar bilateral class II furcation defects (classification of Hamp et al. in mandibular first molars were selected. A split-mouth design was incorporated and the selected 30 furcation defects were assigned to one of the two treatment groups, i.e., Group I treated with a bioresorbable membrane from bovine-derived collagen guided tissue regeneration membrane and Group II treated using porous hydroxyapatite bone graft material on the contralateral sides. Evaluation of clinical parameters, probing depths and attachment levels, and radiographs was done preoperatively and 6 months postoperatively. Results: Both the groups showed statistically significant mean reduction in probing depths and gain in clinical attachment levels and linear bone fill. Comparison between Group I and Group II showed insignificant difference. Conclusion: Within the limits of this study, both the treatment modalities are beneficial for the treatment of human mandibular class II furcation defects.

  2. [Lincomycin applied to the alveolus on TCP carrier and its effect on wound healing after surgical extraction of a third molar].

    Science.gov (United States)

    Wiśniewska, Izabela; Slósarczyk, Anna; Myśliwiec, Leszek; Sporniak-Tutak, Katarzyna

    2009-01-01

    The dental surgeon is often confronted by complications particularly after extraction ofunerupted lower third molars. The most common complication is alveolar periostitis. The healing process after extraction is accompanied by physiologic atrophy of the alveolus involving on the average 30% of bone tissue. Beta-tricalcium phosphate (TCP) is a synthetic material used in medicine to fill up bone defects caused by pathologic processes. The properties of TCP are appropriate for the material to be used as a carrier for drugs, in particular antibiotics. This study was undertaken to determine whether lincomycin applied to the alveolus on TCP carrier can be used to accelerate wound healing and reduce inflammation after surgical extraction of a third molar. We enrolled 80 patients (males and females between the age of 18 and 50 years) who underwent extraction of a third molar at the Department of Dental Surgery, Pomeranian Medical University in Szczecin. Surgical difficulty in the patients according to the Pederson scale corresponded to grade 2 or 3 (medium or high difficulty). The study group consisted of 40 patients who received lincomycin on TCP. Beta-tricalcium phosphate (300-700 microm pores) obtained from the Department of Technology of Ceramics and Refractories, AGH University of Science and Technology in Cracow, was soaked with 500 mg of lincomycin in solution and applied to the dental alveolus after tooth extraction. The alveolus was tightly sutured. The control group comprised 40 patients not treated with lincomycin. The patients reappeared for examination on the first, third, and seventh day after surgery. Attention during follow-up was directed to alveolar periostitis, pain, and trismus. Pain intensity was assessed with the 10-degree Visual Analog Scale (VAS). We analyzed the subjective pain intensity reported during follow-up by the patients. In the study group, 20 patients reported no pain 24 hours after extraction. On the third day after surgery, alveolar

  3. The effects of a topical gel containing chitosan, 0,2% chlorhexidine, allantoin and despanthenol on the wound healing process subsequent to impacted lower third molar extraction.

    Science.gov (United States)

    Madrazo-Jiménez, M; Rodríguez-Caballero, Á; Serrera-Figallo, M-Á; Garrido-Serrano, R; Gutiérrez-Corrales, A; Gutiérrez-Pérez, J-L; Torres-Lagares, D

    2016-11-01

    Despite efforts to prevent postoperative discomfort, there are still many immediate side effects associated with the surgical extraction of impacted lower third molars. Cicatrization is a physiological process through which the loss of integrity of oral mucosa is recovered and damaged tissues are repaired. Bexident Post (ISDIN, Spain) is a topical gel that contains chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol. While this gel has many clinical indications, there are no published clinical trials evaluating its use in impacted mandibular third molar surgery. This study aims to clinically evaluate the efficacy of a gel containing chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol on wound healing and reduction of postoperative side effects and complications after extraction of an impacted mandibular third molar. A split-mouth design study was carried out on a total of 50 bilaterally and symmetrically impacted third molar extractions, which were randomly placed into either a control group (CG=25) or an experimental group (EG=25). Patients were all informed of the purpose of the study and provided written consent. All procedures were carried out by the same dental practitioner, in accordance with standard surgical protocol. A different dental practitioner, unaware of which treatment had been applied, provided follow-up care. The EG applied 10 ml of topical gel composed of chitosan, 0.2% chlorhexidine, allantoin and dexpanthenol to the surgical wound three times a day for 10 days, patients in the CG did not apply any gel. The groups were homogeneous insofar as potentially confounding variables. No significant findings were found regarding postoperative swelling and pain. Neither of the groups displayed poor healing or infectious complications of the wound during the postoperative period. In all the recorded follow-ups (Day 7 p=0.001, and Day 14 p=0.01), the wound's aesthetic appearance was better in the EG. Overall treatment tolerance was satisfactory

  4. A comparison of the effects of midazolam/fentanyl and midazolam/tramadol for conscious intravenous sedation during third molar extraction.

    Science.gov (United States)

    Göktay, Ozgen; Satilmiş, Tülin; Garip, Hasan; Gönül, Onur; Göker, Kamil

    2011-06-01

    This study evaluated the effects of fentanyl and tramadol, used in combination, as sedation for third molar surgical extraction. This prospective, randomized, double-blind, placebo-controlled study included 60 patients undergoing extraction of a horizontal third molar with an Amsterdam Preoperative Anxiety and Information Scale score above 10 points. All of the patients were first given a 0.03-mg/kg bolus of midazolam, and then they were randomized into 3 groups: group A, midazolam only; group B, midazolam and 1-μg/kg fentanyl; and group C, midazolam and 1-mg/kg tramadol. The vital signs were recorded. Patients were assessed for postoperative pain and adverse effects, and patient and surgeon satisfaction was assessed. No differences were found in the heart rate among groups (P > .05). The mean blood pressure was also similar until the 40th minute, after which the mean blood pressure in the patients in group A was lower than that in the other 2 groups (P scale scores of the patients in group C were lower than those in the other 2 groups in the first postoperative hour (P < .05). The time at which the first rescue analgesic was taken in groups A, B, and C was 3, 3.5, and 5 hours postoperatively, respectively, and was significantly later in group C (P < .01). No difference was found in patient/surgeon satisfaction among the groups. Tramadol has a better analgesic effect in third molar surgery than fentanyl and placebo. Copyright © 2011 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  5. Automated Image Analysis in Undetermined Sections of Human Permanent Third Molars

    DEFF Research Database (Denmark)

    Bjørndal, Lars; Darvann, Tron Andre; Bro-Nielsen, Morten

    1997-01-01

    A computerized histomorphometric analysis was made of Karnovsky-fixed, hydroxethylmethacrylate embedded and toluidine blue/pyronin-stained sections to determine: (1) the two-dimensional size of the coronal odontoblasts given by their cytoplasm:nucleus ratio; (2) the ratio between the number...... of coronal odontoblasts and dentinal tubules; and (3) the relation between odontoblast size and adjacent predentine. All conditions were measured in relation to three well-defined sectioning profiles of the dentinal tubules. The sections were randomly taken from 10 unerupted and erupted third-molar crowns...... with respect to odontoblast cell nuclei, cytoplasm and background was computed by statistical discriminant analysis. In longitudinal profiles of the dentinal tubules the cytoplasm:nucleus ratio in erupted teeth was 3.1 +or- 0.54, and the mean of the odontoblast cell:dentinal tubule ratio was 1.19 +or- 0...

  6. Comparing gray mineral trioxide aggregate and diluted formocresol in pulpotomized human primary molars.

    Science.gov (United States)

    Zealand, Cameron M; Briskie, Daniel M; Botero, Tatiana M; Boynton, James R; Hu, Jan C C

    2010-01-01

    The purpose of this multisite, multioperator, prospective, randomized, controlled clinical trial was to evaluate the 6-month outcomes of diluted formocresol (DFC) compared to gray mineral trioxide aggregate (GMTA) as pulpotomy medicament. Determined by a power analysis, 252 molars of 152 children were recruited. The teeth were randomly assigned to receive GMTA or DFC. At the 6-month follow-up, 118 children with 203 treated teeth were evaluated. Four blinded and calibrated evaluators scored each radiograph for pathologies. Clinical success was similar for DFC (97%) and GMTA (100%), (P<.09). Radiographic success differed significantly (P<.04) for DFC (86%) and GMTA (95%). Pulp canal obliteration was radiographically observed in 25% of the DFC group and in 37% of the GMTA group (P=.07). Dentin bridging was observed in 22% of the GMTA group but was not found in the DFC group (P<.01). Teeth treated with GMTA showed more favorable radiographic outcomes than DFC at 6 months post-treatment.

  7. Is The Late Mandibular Fracture From Third Molar Extraction a Risk Towards Malpractice? Case Report with the Analysis of Ethical and Legal Aspects

    Directory of Open Access Journals (Sweden)

    Weuler dos Santos Silva

    2017-06-01

    Full Text Available Objectives: The present study reports a case of late mandibular fracture due to third molar extraction and highlights the inherent clinical, ethical and legal aspects related to this surgical complication. Material and Methods: A female patient underwent surgical procedure for the extraction of the mandibular right third molar. Two days after the surgery the patient reported pain and altered occlusion in the right side of the mandible. After clinical and radiographic re-examination, the diagnosis of late mandibular fracture was established. A second surgery, under general anaesthesia, was performed for the fixation of the mandibular bone. Results: The fractured parts were reduced and fixed with locking plate systems and 2 mm screws following load-sharing principles. The masticatory function showed optimal performance within 7 and 21 days after the surgery. Complete bone healing was observed within 1 year of follow-up. Conclusions: For satisfactory surgical outcomes, adequate surgical planning and techniques must be performed. Signed informed consents explaining the risks and benefits of the treatment must be used to avoid ethical and legal disputes in dentistry.

  8. Clinical comparative study of the effectiveness of two dosages of Dexamethasone to control postoperative swelling, trismus and pain after the surgical extraction of mandibular impacted third molars.

    Science.gov (United States)

    Laureano Filho, Jose Rodrigues; Maurette, Paul Edward; Allais, Marvis; Cotinho, Milane; Fernandes, Caio

    2008-02-01

    The aim of this study was to evaluate the effect of two different concentrations (4 and 8 mg) of dexamethasone to decrease the swelling and trismus after the surgical extraction of mandibular impacted third molars. This randomized clinical trial comprised thirty (30) adult patients of both genders with no local or systemic problems, with bilateral impacted lower third molars in similar position, where surgical extraction had been indicated. They were given 4 mg and 8 mg of dexamethasone 1 hour before the surgical procedure at the first or second surgery. The choice of which side to operate first and the amount of concentration of medication to use was made randomly and double-blindly. Postoperative pain was evaluated using a visual analog scale (VAS) and the degree of swelling was evaluated through facial reference points' variation. The presence of trismus was analyzed through measurement of the interincisal distance (IID). These assessments were obtained before the operation and 24h and 48 h after the surgery. Based on statistic analysis (pared t-student and Wilcoxon tests), the results showed a significant difference in the measurements of the degree of swelling and trismus of the treated sample. 8 mg of dexamethasone promoted a greater reduction of symptoms than 4 mg of dexamethasone The administration of 8 mg of the dexamethasone was more effective than 4 mg of the dexamethasone to reduce the degree of swelling and trismus. However, it had no effect on pain control.

  9. Comparing the incidence of trismus following mandibular third molar extraction after administration of chlorhexidine and normal saline

    Directory of Open Access Journals (Sweden)

    M. Jafari

    1998-04-01

    Full Text Available   In this double blind clinical survey, 40 healthy candidates of mandibular thir molar surgery were selected and randomly allocated into two groups of 20. First group was asked to administer Chlorhexidine 0.2% mouth wash prior to the operation while the other group used normal saline for 60 seconds before the surgery. Mouth opening was measured before surgery and in 1st, 3rd, 7th days following the operation. Statistical analysis revealed significantly more mouth opening (lower level of trismus in patients utilizing chlorhexidine before surgery.

  10. Antimicrobial activity of Calendula officinalis, Camellia sinensis and chlorhexidine against the adherence of microorganisms to sutures after extraction of unerupted third molars

    Directory of Open Access Journals (Sweden)

    Raquel Lourdes Faria

    2011-10-01

    Full Text Available OBJECTIVE: The objective of this study was to compare the antimicrobial effect of mouthwashes containing Calendula officinalis L., Camellia sinensis (L. Kuntze and 0.12% chlorhexidine digluconate on the adherence of microorganisms to suture materials after extraction of unerupted third molars. MATERIAL AND METHODS: Eighteen patients with unerupted maxillary third molars indicated for extraction were selected (n=6 per mouthwash. First, the patients were subjected to extraction of the left tooth and instructed not to use any type of antiseptic solution at the site of surgery (control group. After 15 days, the right tooth was extracted and the patients were instructed to use the Calendula officinalis, Camellia sinensis or chlorhexidine mouthwash during 1 week (experimental group. For each surgery, the sutures were removed on postoperative day 7 and placed in sterile phosphate-buffered saline. Next, serial dilutions were prepared and seeded onto different culture media for the growth of the following microorganisms: blood agar for total microorganism growth; Mitis Salivarius bacitracin sucrose agar for mutans group streptococci; mannitol agar for Staphylococcus spp.; MacConkey agar for enterobacteria and Pseudomonas spp., and Sabouraud dextrose agar containing chloramphenicol for Candida spp. The plates were incubated during 24-48 h at 37ºC for microorganism count (CFU/mL. RESULTS: The three mouthwashes tested reduced the number of microorganisms adhered to the sutures compared to the control group. However, significant differences between the control and experimental groups were only observed for the mouthwash containing 0.12% chlorhexidine digluconate. CONCLUSIONS: Calendula officinalis L. and Camellia sinensis (L. Kuntze presented antimicrobial activity against the adherence of microorganisms to sutures but were not as efficient as chlorhexidine digluconate.

  11. Antimicrobial activity of Calendula officinalis, Camellia sinensis and chlorhexidine against the adherence of microorganisms to sutures after extraction of unerupted third molars

    Science.gov (United States)

    FARIA, Raquel Lourdes; CARDOSO, Lincoln Marcelo Lourenço; AKISUE, Gokithi; PEREIRA, Cristiane Aparecida; JUNQUEIRA, Juliana Campos; JORGE, Antonio Olavo Cardoso; SANTOS JÚNIOR, Paulo Villela

    2011-01-01

    Objective The objective of this study was to compare the antimicrobial effect of mouthwashes containing Calendula officinalis L., Camellia sinensis (L.) Kuntze and 0.12% chlorhexidine digluconate on the adherence of microorganisms to suture materials after extraction of unerupted third molars. Material and Methods Eighteen patients with unerupted maxillary third molars indicated for extraction were selected (n=6 per mouthwash). First, the patients were subjected to extraction of the left tooth and instructed not to use any type of antiseptic solution at the site of surgery (control group). After 15 days, the right tooth was extracted and the patients were instructed to use the Calendula officinalis, Camellia sinensis or chlorhexidine mouthwash during 1 week (experimental group). For each surgery, the sutures were removed on postoperative day 7 and placed in sterile phosphate-buffered saline. Next, serial dilutions were prepared and seeded onto different culture media for the growth of the following microorganisms: blood agar for total microorganism growth; Mitis Salivarius bacitracin sucrose agar for mutans group streptococci; mannitol agar for Staphylococcus spp.; MacConkey agar for enterobacteria and Pseudomonas spp., and Sabouraud dextrose agar containing chloramphenicol for Candida spp. The plates were incubated during 24-48 h at 37ºC for microorganism count (CFU/mL). Results The three mouthwashes tested reduced the number of microorganisms adhered to the sutures compared to the control group. However, significant differences between the control and experimental groups were only observed for the mouthwash containing 0.12% chlorhexidine digluconate. Conclusions Calendula officinalis L. and Camellia sinensis (L.) Kuntze presented antimicrobial activity against the adherence of microorganisms to sutures but were not as efficient as chlorhexidine digluconate. PMID:21986652

  12. The efficacy of 2 different doses of dexamethasone to control postoperative swelling, trismus, and pain after third molar extractions.

    Science.gov (United States)

    Agostinho, Cicero Newton Lemos Felicio; da Silva, Vanessa Camila; Maia Filho, Etevaldo Matos; Cruz, Maria Luiza; Bastos, Eider Guimaraes

    2014-01-01

    This article addresses the effect of 2 different concentrations (4 and 12 mg) of dexamethasone to control pain, swelling, and trismus after third molar surgery. A clinical study was conducted with 27 male and female patients, all presenting with bilaterally displaced mandibular third molars. The treatment protocol required a surgical removal of each tooth in 2 separate operations. The patients were given a preoperative dose of dexamethasone-4 mg for one surgery, 12 mg for the other. The choice of which side would be operated on first and which dose of dexamethasone would be taken was performed randomly, under double-blind conditions. The trismus was assessed by measuring the interincisal distance. Pain intensity was measured both by the amount of painkillers (acetaminophen 750 mg) taken postsurgery and by the Visual Analogue Pain Scale. Data were collected 1 hour preoperative, then at 24 and 48 hours postoperative. A statistical analysis (student's t, Wilcoxon and Friedman tests) of the results showed no significant differences (α = 0.05) between the analyzed variables for the 2 doses of dexamethasone (4 and 12 mg).

  13. The efficacy of chlorhexidine gel in the prevention of alveolar osteitis after mandibular third molar extraction: a systematic review and meta-analysis.

    Science.gov (United States)

    Teshome, Amare

    2017-05-19

    Alveolar osteitis is a very painful and distressing condition for a patient who has recently undergone a tooth extraction and has led dental professionals to search for preventive measures. The aim of this meta-analysis to determine the effect of chlorhexidine (CHX) gel on the incidence of alveolar osteitis after mandibular third molar extraction. Studies were searched for on electronic search engines using Medline (PubMed), Cochrane central, Scopus and advanced Google Scholar from May 2015 to December 2015. Randomized controlled trial studies with a history of mandibular third molar extraction, along with the administration of topical chlorhexidine gel were included. The risk of bias of the selected articles was assessed using the Cochrane risk of bias assessment tool. RevMan 5.3 Software was used to analyze the pooled effect. I(2) was calculated to determine heterogeneity and a funnel plot was used to check the risk of bias. Subgroup analysis was also done based on the presence of confounding factors (smoking, oral contraceptive etc.) and on split mouth design. Out of 52 articles, ten met the inclusion criteria. 862 participants were involved in the selected studies with a mean age range from 24.15 ± 5.02 to 36.65 ± 11. The overall RR was 0.43 (95% CI: 0.32, 0.58, p < 0.00001). Three studies used a split-mouth design to check the effect of chlorhexidine gel in the prevention of alveolar osteitis incidence. There was a pooled effect of 0.29 (95% CI: 0.16, 0.50) for the intervention group in the split mouth design studies. A stratified analysis was done to check the effect of CHX gel in patients with confounding factors and a significant reduction of AO incidence was found; 0.60 (95% CI: 0.41, 0.87; p = 0.05) in the intervention. There was no reported adverse reaction. The heterogeneity (I(2)) was 40%. The funnel plot showed that there was no significant publication bias. This meta-analysis suggests that CHX gel is superior to a placebo in

  14. Effect of hypnosis on induction of local anaesthesia, pain perception, control of haemorrhage and anxiety during extraction of third molars: a case-control study.

    Science.gov (United States)

    Abdeshahi, Seyyed Kazem; Hashemipour, Maryam Alsadat; Mesgarzadeh, Vahid; Shahidi Payam, Akbar; Halaj Monfared, Alireza

    2013-06-01

    Systemic conditions are considered limiting factors for surgical procedures under local anaesthesia in the oral cavity. All the pharmacological methods to control pain in patients have some disadvantages, such as side effects and extra costs for rehabilitation. Therefore, in such cases alternative treatment modalities are considered, such as hypnosis in dentistry. The aim of the present study was to evaluate the effect of hypnosis on haemorrhage, pain and anxiety during the extraction of third molars. In this case-control study, 24 female and male volunteers were included. The subjects had been referred to the Department of Oral and Maxillofacial Surgery, Kerman University of Medical Sciences, for extraction of third molars. Demographic data for all the subjects were recorded. Patients with chronic medical conditions were excluded. The patients were used as their own controls, with the third molars on one side being removed under hypnosis and on the opposite side under local anaesthetic. Hypnosis was induced by one of the two methods, either fixing the gaze on one point or Chiasson's technique; both these methods are appropriate for patients in the dental chair. The Spielberger State-Trait Anxiety Inventory was used to determine patient anxiety levels before hypnosis and anaesthesia. Pain was scored using VAS (visual analogue scale). After surgery the patient was asked to bite on a sterile gauze pad over the surgical site for 30 min when haemorrhage from the area was evaluated. If there was no haemorrhage the patient was discharged. If haemorrhage persisted, the gauze pad was left in place for another 30 min and the area was re-evaluated. Any active oozing from the area after 30 min was considered haemorrhage. Haemorrhage, anxiety and pain were compared between the two groups. Data was analyzed using the t-test, McNemar's test and Wilcoxon's signed rank test using SPSS 18 statistical software. Twenty-four patients were evaluated; there were 14 males (58.3%) and 10

  15. Comparing Gray Mineral Trioxide Aggregate and Diluted Formocresol in Pulpotomized Human Primary Molars

    Science.gov (United States)

    Zealand, Cameron M.; Briskie, Daniel M.; Botero, Tatiana M.; Boynton, James R.; Hu, Jan C.C.

    2016-01-01

    Purpose The purpose of this multisite, multioperator, prospective, randomized, controlled clinical trial was to evaluate the 6-month outcomes of diluted formocresol (DFC) compared to gray mineral trioxide aggregate (GMTA) as pulpotomy medicament. Methods Determined by a power analysis, 252 molars of 152 children were recruited. The teeth were randomly assigned to receive GMTA or DFC. At the 6-month follow-up, 118 children with 203 treated teeth were evaluated. Results Four blinded and calibrated evaluators scored each radiograph for pathologies. Clinical success was similar for DFC (97%) and GMTA (100%), (P<.09). Radiographic success differed significantly (P<.04) for DFC (86%) and GMTA (95%). Pulp canal obliteration was radiographically observed in 25% of the DFC group and in 37% of the GMTA group (P=.07). Dentin bridging was observed in 22% of the GMTA group but was not found in the DFC group (P<.01). Conclusion Teeth treated with GMTA showed more favorable radiographic outcomes than DFC at 6 months post-treatment. PMID:21070705

  16. Plasma variations in stress markers: Clinical trial of two anesthetics used in regional block in the extraction of impacted inferior third molars

    Science.gov (United States)

    Arteagoitia, Iciar; Zumarraga, Mercedes; Dávila, Ricardo; Barbier, Luis; Santamaría, Gorka

    2014-01-01

    Objectives: Was to evaluate the effect of different regional anesthetics (articaine with epinephrine versus prilocaine with felypressin) on stress in the extraction of impacted lower third molars in healthy subjects. Sutdy Desing: A prospective single-blind, split-mouth cross-over randomized study was designed, with a control group. The experimental group consisted of 24 otherwise healthy male volunteers, with two impacted lower third molars which were surgically extracted after inferior alveolar nerve block (regional anesthesia), with a fortnight’s interval: the right using 4% articaine with 1:100.000 epinephrine, and the left 3% prilocaine with 1:1.850.000 felypressin. Patients were randomized for the first surgical procedure. To analyze the variation in four stress markers, homovanillic acid, 3-methoxy-4-hydroxyphenylglycol, prolactin and cortisol, 10-mL blood samples were obtained at t = 0, 5, 60, and 120 minutes. The control group consisted of 12 healthy volunteers, who did not undergo either extractions or anesthetic procedures but from whom blood samples were collected and analyzed in the same way. Results: Plasma cortisol increased in the experimental group (multiple range test, P<0.05), the levels being significantly higher in the group receiving 3% prilocaine with 1:1.850,000 felypressin (signed rank test, p<0.0007). There was a significant reduction in homovanillic acid over time in both groups (multiple range test, P<0.05). No significant differences were observed in homovanillic acid, 3-methoxy-4-hydroxyphenylglycol or prolactin concentrations between the experimental and control groups. Conclusions: The effect of regional anesthesia on stress is lower when 4% articaine with 1:100,000 epinephrine is used in this surgical procedure. Key words:Stress markets, epinephrine versus felypressin. PMID:24316704

  17. Mandibular Third Molar Impaction: Review of Literature and a Proposal of a Classification

    Directory of Open Access Journals (Sweden)

    Gintaras Juodzbalys

    2013-06-01

    Full Text Available Objectives: The purpose of present article was to review impacted mandibular third molar aetiology, clinical anatomy, radiologic examination, surgical treatment and possible complications, as well as to create new mandibular third molar impaction and extraction difficulty degree classification based on anatomical and radiologic findings and literature review results.Material and Methods: Literature was selected through a search of PubMed, Embase and Cochrane electronic databases. The keywords used for search were mandibular third molar, impacted mandibular third molar, inferior alveolar nerve injury third molar, lingual nerve injury third molar. The search was restricted to English language articles, published from 1976 to April 2013. Additionally, a manual search in the major anatomy and oral surgery journals and books was performed. The publications there selected by including clinical and human anatomy studies.Results: In total 73 literature sources were obtained and reviewed. Impacted mandibular third molar aetiology, clinical anatomy, radiographic examination, surgical extraction of and possible complications, classifications and risk factors were discussed. New mandibular third molar impaction and extraction difficulty degree classification based on anatomical and radiologic findings and literature review results was proposed. Conclusions: The classification proposed here based on anatomical and radiological impacted mandibular third molar features is promising to be a helpful tool for impacted tooth assessment as well as for planning for surgical operation. Further clinical studies should be conducted for new classification validation and reliability evaluation.

  18. Mandibular third molar impaction: review of literature and a proposal of a classification.

    Science.gov (United States)

    Juodzbalys, Gintaras; Daugela, Povilas

    2013-07-01

    The purpose of present article was to review impacted mandibular third molar aetiology, clinical anatomy, radiologic examination, surgical treatment and possible complications, as well as to create new mandibular third molar impaction and extraction difficulty degree classification based on anatomical and radiologic findings and literature review results. Literature was selected through a search of PubMed, Embase and Cochrane electronic databases. The keywords used for search were mandibular third molar, impacted mandibular third molar, inferior alveolar nerve injury third molar, lingual nerve injury third molar. The search was restricted to English language articles, published from 1976 to April 2013. Additionally, a manual search in the major anatomy and oral surgery journals and books was performed. The publications there selected by including clinical and human anatomy studies. In total 75 literature sources were obtained and reviewed. Impacted mandibular third molar aetiology, clinical anatomy, radiographic examination, surgical extraction of and possible complications, classifications and risk factors were discussed. New mandibular third molar impaction and extraction difficulty degree classification based on anatomical and radiologic findings and literature review results was proposed. The classification proposed here based on anatomical and radiological impacted mandibular third molar features is promising to be a helpful tool for impacted tooth assessment as well as for planning for surgical operation. Further clinical studies should be conducted for new classification validation and reliability evaluation.

  19. Efficacy of naproxen with or without esomeprazole for pain and inflammation in patients after bilateral third molar extractions: A double blinded crossover study

    Science.gov (United States)

    Weckwerth, Giovana M.; Simoneti, Luis F.; Zupelari-Gonçalves, Paulo; Calvo, Adriana M.; Brozoski, Daniel T.; Dionísio, Thiago J.; Torres, Elza A.; Lauris, José-Roberto P.; Faria, Flávio-Augusto C.

    2017-01-01

    Background Using a double-blinded randomized crossover design, this study aimed to evaluate acute postoperative pain management, swelling and trismus in 46 volunteers undergoing extractions of the two lower third molars, in similar positions, at two different appointments who consumed a tablet of either NE (naproxen 500 mg + esomepraz ole 20 mg) or only naproxen (500 mg) every 12 hours for 4 days. Material and Methods Parameters were analyzed: self-reported pain intensity using a visual analog scale (VAS) pre- and postoperative mouth opening; incidence, type and severity of adverse reactions; total quantity consumed of rescue medication; and pre- and postoperative swelling. Results Female volunteers reported significantly more postoperative pain at 1, 1.5, 2, 3 and 4hrs after surgery while also taking their first rescue medication at a time significantly earlier when consuming NE when compared to naproxen (3.7hrs and 6.7hrs). Conversely, no differences were found between each drug group in males. Conclusions In conclusion, throughout the entire study, pain was mild after using either drug in both men and women with pain scores on average well below 40mm (VAS), although in women naproxen improved acute postoperative pain management when compared to NE. Key words:Oral surgery, third molar, pain, naproxen, esomeprazole, NSAIDs. PMID:27918744

  20. Efficacy of 2% mepivacaine and 2% lignocaine in the surgical extraction of mesioangular angulated bilaterally impacted third molars: A double-blind, randomized, clinical trial.

    Science.gov (United States)

    Barath, S; Triveni, V S S; Sai Sujai, G V Naga; Harikishan, G

    2015-08-01

    The purpose of this prospective, randomized, double-blind study was to compare the anesthetic efficacy of 2% mepivacaine and 2% lidocaine (both with 1:80,000 epinephrine) for inferior alveolar nerve block in mesioangular bilaterally impacted third molar extraction. Forty patients with mesioangular bilaterally impacted third molars were taken for the study; either 2% mepivacaine or 2% lidocaine is given in a double-blind manner. Surgery started 5 min after solution deposition. Success was defined as no or mild discomfort (visual analog scale [VAS] recordings) during the surgical procedure. The mean time for onset period 4.2 min and 4.6 min (P = 0.018). The mean duration anesthesia 177.17 min 166.71 min (P = 0.085). No significant difference between the scores of pain reported by the patients by VAS and venovenous bypass treated with mepivacaine and lidocaine (P = 0.000). Slight increased postoperative analgesics required for mepivacaine group (4.000 tablets) and lidocaine group (4.170 tablets) (P = 0.335). The sharp increase of pulse rate with respect to both the solutions at 5 min after postinjection of local anesthetics. However, there was no statically significant difference in systolic and diastolic blood (P = 0.681) and (P = 0.270). Lidocaine and mepivacaine with the same vasoconstrictor have similar action and both solutions are effective in surgical procedures. There were also no significant differences between them in relation to the intensity of postoperative pain.

  1. Comparison of dental measurement systems for taxonomic assignment of Neanderthal and modern human lower second deciduous molars.

    Science.gov (United States)

    Benazzi, Stefano; Fornai, Cinzia; Bayle, Priscilla; Coquerelle, Michael; Kullmer, Ottmar; Mallegni, Francesco; Weber, Gerhard W

    2011-09-01

    Traditional morphometric approaches for taxonomic assignment of Neanderthal and modern human dental remains are mainly characterized by caliper measurements of tooth crowns. Several studies have recently described differences in dental tissue proportions and enamel thickness between Neanderthal and modern human teeth. At least for the lower second deciduous molar (dm(2)), a three-dimensional lateral relative enamel thickness index has been proposed for separating the two taxa. This index has the advantage over other measurements of being applicable to worn teeth because it ignores the occlusal aspect of the crown. Nevertheless, a comparative evaluation of traditional crown dimensions and lateral dental tissue proportion measurements for taxonomic assignment of Neanderthal and modern human dm(2)s has not yet been performed. In this study, we compare various parameters gathered from the lateral aspects of the crown. These parameters include crown diameters, height of the lateral wall of the crown (lateral crown height = LCH), lateral enamel thickness, and dentine volume of the lateral wall, including the volume of the coronal pulp chamber (lateral dentine plus pulp volume = LDPV), in a 3D digital sample of Neanderthal and modern human dm(2)s to evaluate their utility in separating the two taxa. The LDPV and the LCH allow us to discriminate between Neanderthals and modern humans with 88.5% and 92.3% accuracy, respectively. Though our results confirm that Neanderthal dm(2)s have lower relative enamel thickness (RET) index compared with modern humans (p = 0.005), only 70% of the specimens were correctly classified on the basis of the RET index. We also emphasize that results of the lateral enamel thickness method depend on the magnitude of the interproximal wear. Accordingly, we suggest using the LCH or the LDPV to discriminate between Neanderthal and modern human dm(2)s. These parameters are more independent of interproximal wear and loss of lateral enamel.

  2. In vitro evaluation of a passive radio frequency identification microchip implanted in human molars subjected to compression forces, for forensic purposes of human identification

    Directory of Open Access Journals (Sweden)

    Freddy Moreno

    2013-01-01

    Full Text Available Objective: To evaluate the in vitro behavior of a passive Radio Frequency Identification (RFID microchip implanted in human molars subjected to compression forces to determine its technical and clinical viability. Materials and Methods: I n vitro experimental study to evaluate the physical behavior of a passive RFID microchip (VeriChip™ implanted in human molars through resin restoration (Filtek P90™ Silorane 3M-ESPE ® to determine the clinical and technical possibilities of the implant and the viability to withstand compression forces exerted by the stomatognathic system during mastication. Results: Through the ANOVA test, it was found that the teeth on which a microchip was implanted show great resistance to compressive forces. It was also evident that teeth with microchips implanted in Class V cavities are more resistant than those implanted in Class I cavities. Conclusions: Although microchip dimensions are big, requiring a sufficiently large cavity, from the biomechanical point of view it is plausible to implant a microchip in a Class V cavity employing restoration material based on resin for forensic purposes of human identification.

  3. Selenium and mercury molar ratios in saltwater fish from New Jersey: Individual and species variability complicate use in human health fish consumption advisories☆

    Science.gov (United States)

    Burger, Joanna; Gochfeld, Michael

    2014-01-01

    Balancing risk versus benefits to humans and other organisms from consuming fish is a national concern in the USA, as well as in many other parts of the world. Protecting public health is both a federal and state responsibility, and states respond by issuing fish consumption advisories, particularly for mercury. Recently it has been emphasized that the protective role of selenium against mercury toxicity depends on their molar ratios, which should be evaluated as an indication of selenium’s protective capacity, and incorporated in risk assessments for fish consumption. However, there is no single “protective” ratio agreed upon. In this paper we examine the selenium:mercury (Se:Hg) molar ratios in a wide range of saltwater fish caught and eaten by recreational fishers along the New Jersey coast. We were particularly interested in interspecific and intraspecific variability, and whether the molar ratios were consistent within a species, allowing for its use in managing risk. The selenium–mercury molar ratio showed significant variation among and within fish species. The molar ratio decreased with the size of the fish species, decreased with the mercury levels, and within a fish species, the selenium:mercury ratio decreased with fish size. As an essential element, selenium undergoes some homeostatic regulation, but it is also highly toxic. Within species, mercury level tends to increase with size, accounting for the negative relationship between size and ratio. This variability may make it difficult to use the selenium:mercury molar ratio in risk assessment, risk management, and risk communication at this time, and more information is needed on how mercury and selenium actually interact and on the relationship between the molar ratios and health outcomes. PMID:22405995

  4. Efficacy of platelet-rich plasma applied to post-extraction retained lower third molar alveoli. A systematic review

    OpenAIRE

    Barona Dorado, Cristina; González Regueiro, Iria; Martín Ares, María; Arias Irimia, Óscar; Martínez González, José María

    2014-01-01

    Dental retentions have a high prevalence among the general population and their removal can involve multiple complications. The use of platelet rich plasma has been proposed in an attempt to avoid these complications, as it contains high growth factors and stimulates diverse biological functions that facilitate the healing of soft and hard tissues. Objectives: To evaluate the available scientific evidence related to the application of platelet-rich plasma in the post-extraction alveoli of a r...

  5. Comparison of the efficacy and safety of 2% lidocaine HCl with different epinephrine concentration for local anesthesia in participants undergoing surgical extraction of impacted mandibular third molars: A multicenter, randomized, double-blind, crossover, phase IV trial.

    Science.gov (United States)

    Karm, Myong-Hwan; Park, Fiona Daye; Kang, Moonkyu; Kim, Hyun Jeong; Kang, Jeong Wan; Kim, Seungoh; Kim, Yong-Deok; Kim, Cheul-Hong; Seo, Kwang-Suk; Kwon, Kyung-Hwan; Kim, Chul-Hwan; Lee, Jung-Woo; Hong, Sung-Woon; Lim, Mi Hyoung; Nam, Seung Kwan; Cho, Jae Min

    2017-05-01

    The most commonly impacted tooth is the third molar. An impacted third molar can ultimately cause acute pain, infection, tumors, cysts, caries, periodontal disease, and loss of adjacent teeth. Local anesthesia is employed for removing the third molar. This study aimed to evaluate the efficacy and safety of 2% lidocaine with 1:80,000 or 1:200,000 epinephrine for surgical extraction of bilateral impacted mandibular third molars. Sixty-five healthy participants underwent surgical extraction of bilateral impacted mandibular third molars in 2 separate visits while under local anesthesia with 2% lidocaine with different epinephrine concentration (1:80,000 or 1:200,000) in a double-blind, randomized, crossover trial. Visual analog scale pain scores obtained immediately after surgical extraction were primarily evaluated for the 2 groups receiving different epinephrine concentrations. Visual analog scale pain scores were obtained 2, 4, and 6 hours after administering an anesthetic. Onset and duration of analgesia, onset of pain, intraoperative bleeding, operator's and participant's overall satisfaction, drug dosage, and hemodynamic parameters were evaluated for the 2 groups. There were no statistically significant differences between the 2 groups in any measurements except hemodynamic factors (P >.05). Changes in systolic blood pressure and heart rate following anesthetic administration were significantly greater in the group receiving 1:80,000 epinephrine than in that receiving 1:200,000 epinephrine (P ≤.01). The difference in epinephrine concentration between 1:80,000 and 1:200,000 in 2% lidocaine liquid does not affect the medical efficacy of the anesthetic. Furthermore, 2% lidocaine with 1:200,000 epinephrine has better safety with regard to hemodynamic parameters than 2% lidocaine with 1:80,000 epinephrine. Therefore, we suggest using 2% lidocaine with 1:200,000 epinephrine rather than 2% lidocaine with 1:80,000 epinephrine for surgical extraction of impacted

  6. Painless minimally invasive surgery versus chisel osteotomy in the extraction of impacted mandibular third molars%无痛微创法与凿骨劈冠法拔除下颌阻生智齿的临床观察

    Institute of Scientific and Technical Information of China (English)

    李然; 唐小剑; 杨震; 朱正宏

    2012-01-01

    目的 观察无痛微创法和凿骨劈冠法拔除下颌阻生智齿的临床疗效.方法 306颗下颌阻生智齿随机分成2组,分别采用无痛微创法(实验组)和凿骨劈冠法(对照组)拔除患牙,记录2组平均拔除时间、术后肿胀、疼痛、张口受限、颞颌关节痛、干槽症等并发症发生情况及对拔牙的畏惧感.结果 实验组与对照组相比,平均拔除时间明显缩短(P<0.01);术后疼痛、肿胀、张口受限、颞颌关节痛、干槽症等并发症发生率较对照组明显降低(P<0.01);术前畏惧在术后的消失率和术前不畏惧在术后出现的畏惧率,均表明实验组优于对照组(P<0.001).结论 随着社会-心理-生物医学模式的不断完善,拔牙手术正由传统的凿骨劈冠法向着无痛、微创、安全、规范、舒适、愉悦等人性化方向发展.%Objective To compare the clinical effects of minimally invasive surgery ( MIS) with those of traditional chisel osteotomy in the extraction of impacted mandibular third molars. Methods We extracted 306 impacted mandibular third molars by MIS (experimental group) and traditional chisel osteotomy (control group) , respectively, and compared the two groups in the operation time, patients'fear of tooth extraction and postoperative complications, such as swelling, pain, limitation of mouth opening, sore temporoman-dibular joint and dry socket. Results Compared with the controls, the experimental group showed significantly shorter operation time, lower rates of pain, swelling, limitation of mouth opening, sore temporomandibular joint, dry socket, and less fear of tooth extraction (P <0. 01). Conclusions MIS is evidently superior over chisel osteotomy in the extraction of impacted mandibular third molars. With the development of the model of socio-psycho-bio-medicine, tooth extraction is assuming more humanized characteristics as pain-lessness, invasiveness, safety, comfort and normalization.

  7. Modeling and remodeling of human extraction sockets.

    Science.gov (United States)

    Trombelli, Leonardo; Farina, Roberto; Marzola, Andrea; Bozzi, Leopoldo; Liljenberg, Birgitta; Lindhe, Jan

    2008-07-01

    The available studies on extraction wound repair in humans are affected by significant limitations and have failed to evaluate tissue alterations occurring in all compartments of the hard tissue defect. To monitor during a 6-month period the healing of human extraction sockets and include a semi-quantitative analysis of tissues and cell populations involved in various stages of the processes of modeling/remodeling. Twenty-seven biopsies, representative of the early (2-4 weeks, n=10), intermediate (6-8 weeks, n=6), and late phase (12-24 weeks, n=11) of healing, were collected and analysed. Granulation tissue that was present in comparatively large amounts in the early healing phase of socket healing, was in the interval between the early and intermediate observation phase replaced with provisional matrix and woven bone. The density of vascular structures and macrophages slowly decreased from 2 to 4 weeks over time. The presence of osteoblasts peaked at 6-8 weeks and remained almost stable thereafter; a small number of osteoclasts were present in a few specimens at each observation interval. The present findings demonstrated that great variability exists in man with respect to hard tissue formation within extraction sockets. Thus, whereas a provisional connective tissue consistently forms within the first weeks of healing, the interval during which mineralized bone is laid down is much less predictable.

  8. Thermal And Spectroscopic Analyses Of Next Generation Caustic Side Solvent Extraction Solvent Contacted With 3, 8, And 16 Molar Nitric Acid

    Energy Technology Data Exchange (ETDEWEB)

    Fondeur, F. F.; Fink, S. D.

    2011-12-07

    A new solvent system referred to as Next Generation Solvent or NGS, has been developed at Oak Ridge National Laboratory for the removal of cesium from alkaline solutions in the Caustic Side Solvent Extraction process. The NGS is proposed for deployment at MCU{sup a} and at the Salt Waste Processing Facility. This work investigated the chemical compatibility between NGS and 16 M, 8 M, and 3 M nitric acid from contact that may occur in handling of analytical samples from MCU or, for 3 M acid, which may occur during contactor cleaning operations at MCU. This work shows that reactions occurred between NGS components and the high molarity nitric acid. Reaction rates are much faster in 8 M and 16 M nitric acid than in 3 M nitric acid. In the case of 16 M and 8 M nitric acid, the nitric acid reacts with the extractant to produce initially organo-nitrate species. The reaction also releases soluble fluorinated alcohols such as tetrafluoropropanol. With longer contact time, the modifier reacts to produce a tarry substance with evolved gases (NO{sub x} and possibly CO). Calorimetric analysis of the reaction product mixtures revealed that the organo-nitrates reaction products are not explosive and will not deflagrate.

  9. Size variation in early human mandibles and molars from Klasies River, South Africa: comparison with other middle and late Pleistocene assemblages and with modern humans.

    Science.gov (United States)

    Royer, Danielle F; Lockwood, Charles A; Scott, Jeremiah E; Grine, Frederick E

    2009-10-01

    Previous studies of the Middle Stone Age human remains from Klasies River have concluded that they exhibited more sexual dimorphism than extant populations, but these claims have not been assessed statistically. We evaluate these claims by comparing size variation in the best-represented elements at the site, namely the mandibular corpora and M(2)s, to that in samples from three recent human populations using resampling methods. We also examine size variation in these same elements from seven additional middle and late Pleistocene sites: Skhūl, Dolní Vestonice, Sima de los Huesos, Arago, Krapina, Shanidar, and Vindija. Our results demonstrate that size variation in the Klasies assemblage was greater than in recent humans, consistent with arguments that the Klasies people were more dimorphic than living humans. Variation in the Skhūl, Dolní Vestonice, and Sima de los Huesos mandibular samples is also higher than in the recent human samples, indicating that the Klasies sample was not unusual among middle and late Pleistocene hominins. In contrast, the Neandertal samples (Krapina, Shanidar, and Vindija) do not evince relatively high mandibular and molar variation, which may indicate that the level of dimorphism in Neandertals was similar to that observed in extant humans. These results suggest that the reduced levels of dimorphism in Neandertals and living humans may have developed independently, though larger fossil samples are needed to test this hypothesis.

  10. Mineral content in teeth with Deciduous Molar Hypomineralisation (DMH)

    NARCIS (Netherlands)

    Elfrink, M.E.C.; ten Cate, J.M.; van Ruijven, L.J.; Veerkamp, J.S.J.

    2013-01-01

    Objectives We report the mineral (hydroxyapatite) density of sound and opaque areas in DMH molars with sound parts of (carious) deciduous teeth serving as controls. Methods Twenty-nine extracted second primary molars obtained from 15 children were studied. Thirteen of these molars were DMH molars

  11. A Systematic Review on Effect of Single-Dose Preoperative Antibiotics at Surgical Osteotomy Extraction of Lower Third Molars

    DEFF Research Database (Denmark)

    Marcussen, Karoline Brørup; Laulund, Anne Sofie; Jørgensen, Henrik L

    2016-01-01

    surgical extraction implying osteotomy. MATERIALS AND METHODS: The Medline, Cochrane Library, and Embase databases were searched for RCTs until August 2015. The primary outcome measure was postoperative inflammatory reactions, with a subgroup analysis of surgical site infection (SSI) and alveolar osteitis....... A risk-of-bias assessment of the included trials was done according to the Cochrane guidelines. RESULTS: A total of 53 RCTs were identified; however, only 10 could be included in the present review. A meta-analysis of the 10 trials showed a statistically significant reduction in SSI and alveolar osteitis...... when antibiotics had been used (odds ratio [OR] = 0.30; 95% confidence interval [CI], 0.19 to 0.47; P ≤ .00001). A subgroup meta-analysis of 6 trials showed that preoperative administration of antibiotics perorally or intravenously significantly reduced the incidence of SSI (OR = 0.19; 95% CI, 0...

  12. Extraction of Chemical Speciation and Molar Absorption Coeficients with Well-posed Solutions of Beer's Law

    Energy Technology Data Exchange (ETDEWEB)

    Boily, Jean F.; Suleimenov, Oleg M.

    2006-06-01

    The ability of two different approaches in retrieving thermodynamic and spectroscopic properties of chemical species, using Beer’s law, was investigated on a suite of synthetic uv-vis spectra generated at various degrees of random error. Analytical and numerical solutions to direct solutions to Beer’s law were compared to solutions starting from orthogonalised absorbance matrices filtered from random noise by the method of Singular Value Decomposition. Solutions making use of absorbance matrices filtered from random noise were the most successful at retrieving properties of chemical species at larger errors. Repeated automated simulation however indicated such solutions to display a number of occurrences associated to poor fits to the synthetic data while direct solutions to Beer’s law produced more consistent sets of values. A bootstrap statistical analysis of the repeated simulations showed the median quality of the fit (e.g. sum-of-squares of the deviations) to be nonetheless far superior for solutions making use of error/noise filtration than those of direct solutions to Beer’s law. These former solutions are therefore recommended for the extraction of spectroscopic and thermodynamic/kinetic properties of chemical species from spectroscopic data, however not without a rigorous validation of the model.

  13. Programa de extrações seriadas: variáveis relacionadas com a extração de pré-molares Serial extraction: variables associated to the extraction of premolars

    Directory of Open Access Journals (Sweden)

    Tulio Silva Lara

    2011-10-01

    Full Text Available OBJETIVO: verificar o percentual de pacientes que necessitaram extração de dentes permanentes, pré-molares, dentre aqueles tratados com extração de dentes decíduos para correção do apinhamento primário na dentição mista, bem como analisar as possíveis variáveis relacionadas. MÉTODOS: a amostra foi composta por documentações ortodônticas de 70 pacientes na dentição permanente, cujo tratamento iniciou-se na dentição mista com planejamento de um programa de extrações seriadas (PES. Todos os prontuários foram analisados por um único examinador, no intuito de verificar se o PES havia sido cumprido com a extração de dentes permanentes ou se havia sido realizada apenas extração de dentes decíduos. Verificou-se a associação entre a extração de dentes permanentes e as variáveis padrão facial; relação sagital entre as arcadas dentárias; IMPA; proporção tamanho do segundo molar permanente inferior/espaço retromolar; mecânica de controle de espaço e discrepância de modelo (teste exato de Fisher para as variáveis categóricas e modelo de regressão logística para as variáveis numéricas. Os resultados foram considerados para pOBJECTIVE: To verify the amount of patients who had to undergo extraction of permanent teeth (premolars in a population of patients treated with extraction of deciduous teeth for correction of incisor crowding in the mixed dentition, as well as to analyze the possibly related variables. METHODS: The sample was composed of orthodontic records of 70 patients in the permanent dentition whose treatment included the serial extraction program (SEP in the mixed dentition. All records were analyzed by a single examiner in order to verify whether the SEP had been carried out with extraction of either permanent teeth or deciduous teeth only. The association between extraction of permanent teeth and the variables lateral facial pattern, sagittal relationship between the dental arches, IMPA

  14. Deciduous molar hypomineralization and molar incisor hypomineralization

    NARCIS (Netherlands)

    Elfrink, M.E.C.; ten Cate, J.M.; Jaddoe, V.W.V.; Hofman, A.; Moll, H.A.; Veerkamp, J.S.J.

    2012-01-01

    This study was embedded in the Generation R Study, a population-based prospective cohort study from fetal life until young adulthood. This study focused on the relationship between Deciduous Molar Hypomineralization (DMH) and Molar Incisor Hypomineralization (MIH). First permanent molars develop dur

  15. Effects of pre-operative submucosal dexamethasone injection on the postoperative swelling and trismus following surgical extraction of mandibular third molar.

    Science.gov (United States)

    Ehsan, Afeefa; Ali Bukhari, Syed Gulzar; Ashar; Manzoor, Arslan; Junaid, Muhammad

    2014-07-01

    To determine the effects of pre-operative submucosal dexamethasone injection on postoperative swelling and trismus following surgical extraction of mandibular third molar. Randomized controlled trial. Department of Oral and Maxillofacial Surgery, Armed Forces Institute of Dentistry (AFID), Rawalpindi, from October 2009 to March 2010. A total of 100 patients aged 18 - 40 years with good periodontal health and mesioangular impaction were divided in two treatment groups (50 in each group). Group-A received prophylactic 4 mg submucosal dexamethasone intraoral injection and Group-B acted as control group. Facial swelling and trismus were assessed at baseline, 2nd and 7th postoperative days. Data was analyzed using SPSS-10. There were 35 (70%) males and 15 (30%) females in group-A and 34 (68%) males and 16 (32%) females in group-B. Surgical time ranged from 30 - 50 minutes (mean = 40.62 ± 4.886 minutes) for group-A and 33 - 50 minutes (mean = 42.12 ± 4.543 minutes) for group-B. Administration of dexamethasone had statistically significant effect in reduction of swelling and trismus on second postoperative day (p trismus.

  16. Comparative Evalution of G bone (Hydroxyapatite) and G-Graft (Hydroxyapatite with Collagen) as Bone Graft Material in Mandibular III Molar Extraction Socket.

    Science.gov (United States)

    Panday, Vijayendra; Upadhyaya, Vivekananda; Berwal, Vikas; Jain, Kapil; Sah, Nupur; Sarathi, Partha; Swami, Pushp Chander

    2015-03-01

    Bone grafting is a dynamic phenomenon. It is a surgical procedure that replaces missing bone with material either from patient's own body, or, an artificial, synthetic or natural substitute. A successful bone graft when applied, heals, becomes incorporated, re-vascularises and eventually assumes the form desired. The main purpose of this present study was to radiologically assess and compare the regenerative potential of hydroxyapatite with Collagen (G-Graft) and hydroxyapatite (G-Bone) and to evaluate the clinical usefulness of these materials to enhance bone healing in third molar extraction sites through bone formation. The study was carried out in the Department of Oral & Maxillofacilal Surgery, patients were divided into three groups. The rationale for assigning the patients to the groups was strictly random: Group I - G-Graft (Hydroxyapatite with Collagen) was used as Bone graft material, Group II - Bone graft material used was G-Bone (Hydroxyapatite), Group III-control group (no grafts was used). Orthopentomogram(OPG) images were taken intra-operatively, just after extraction in the Group III (control), after extraction but before graft placement in Group I & II (study groups) and post-operatively at the end of first month and third month. Bone density of the post-extraction sockets was measured at four random areas through 'densitometric analysis' software in the OPG program (Kodak 8000C Digital Panoramic System, Eastman Kodak Company) and an average value was recorded at each review. The percentage increase in bone density between 1(st) month & 3(rd) month was 7.55± 12.43 in Group I (G Graft), 4.41± 5.4859 in Group II (G Bone), while that Group III (control) was found to be -0.82 ± 3.96. The bone density increase was found to be statistically highly significant (phealing without provoking any significant inflammatory process. The study also indicates that defects treated with G-Graft attain more density initially and that G-Graft enhances bone healing

  17. Impacted lower third molars and distal caries in the mandibular second molar. Is prophylactic removal of lower third molars justified?

    Science.gov (United States)

    Marques, José; Montserrat-Bosch, Marta; Vilchez-Pérez, Miguel-Angel; Valmaseda-Castellón, Eduard; Gay-Escoda, Cosme

    2017-01-01

    Background The objective of this study was to evaluate the association between the presence of mandibular third molars and the occurrence of carious lesions in the distal aspect of the mandibular second molar. Material and Methods A retrospective cohort study comprising 327 lower third molars extracted in the Oral Surgery and Implantology Master’s Degree program of the School of Dentistry of the University of Barcelona (Barcelona, Spain) was carried out. A descriptive and bivariate analysis was made. The diagnosis of caries in the second molar and the position of the mandibular third molar were evaluated through panoramic radiographies. Results The sample included 203 patients, 94 males (46.3%) and 109 females (53.7%), with a mean age of 26,8 years and 327 lower third molars. The prevalence of second molar distal caries was 25.4% (95% CI= 20.6% to 30.2%). This pathology was significantly more frequent when the third molar was in a horizontal position (27.7%), when the contact point was at (45,8%) or below (47.0%) the cementoenamel junction (CEJ), and when the distal CEJ of the mandibular second molar and the mesial CEJ of the third molar was 7 to 12 mm apart. Conclusions Horizontal lower third molars with contact points at or below the CEJ are more likely to produce distal caries in the mandibular second molars. Due to the high prevalence of this pathology (20.6% to 30.2%), a prophylactic removal of lower third molars with the above-mentioned features might be advisable. Key words:Second molar, caries, third molar, prophylactic removal. PMID:28638558

  18. 下颌阻生第三磨牙拔除术对下颌第二磨牙远中牙周组织的影响%The influence of the impacted mandibular third molar extraction on the distal periodontal tissue of the mandibu- lar second molar

    Institute of Scientific and Technical Information of China (English)

    许竞

    2016-01-01

    下颌阻生第三磨牙拔除后可能遗留下颌第二磨牙远中根的牙周组织缺损,其发生及预后受年龄、第三磨牙的阻生状态、术前局部的感染情况、术前邻牙状况等因素的影响. 拔牙时年龄较大、低位阻生、术前存在冠周感染或反复食物嵌塞、邻牙龋坏等因素将使这种牙周骨质缺损加剧且预后不佳. 从预防的角度,尽可能在小于25岁的低龄期拔牙,有利于阻止这种不良反应的发生或加剧;进行骨质移植可以有效促进术后第二磨牙远中牙周骨质缺损的修复.%The periodontal bone defect might be formed on the distal surface of the second molar root followed the ex-traction of the mandibular third molar. The occurrence and prognosis of the complication would be influenced by the age of extraction, the impaction level of the third molar, local inflammation, health state of adjacent tooth, etc. The perio-dontal bone defect of the second molar would be severer and had poorer prognosis in the case of extraction for the older patients, deeper impaction of the third molar, local inflammation, food impaction, adjacent tooth caries. From the proph-ylactics, it was beneficial for the prevention of the complication or reducing severity of the complication when the extrac-tion of lower third molar was performed before the age of 25 years old. The periodontal bone defect could be effectively re-paired by transplanting bone graft.

  19. Effects of second premolar extractions on third molar angulations%正畸拔除第二前磨牙后第三磨牙倾斜度的变化

    Institute of Scientific and Technical Information of China (English)

    陈志明

    2009-01-01

    近年来,正畸的研究在口腔医学界日益受到重视,该文总结了拔牙矫治和非拔牙矫治,拔除前磨牙正畸治疗能够改善第三磨牙倾斜度,期望对相关临床和科研人员有所帮助.%In recent years, the study of orthodontics was achieved more attentions in stomatology. The extraction or non-extraction orthodontic treatment were summarized. Extraction of premolars orthodontic treatment can improve the third molar inclination. This might be useful in clinical application.

  20. POSTOPERATIVE HEMOSTASIS AFTER EXTRACTION OF RETENTION DECIDUOUS INCISORS AND MOLARS%滞留乳切牙与乳磨牙拔除术后止血状况观察

    Institute of Scientific and Technical Information of China (English)

    姜松磊; 王耀钟; 卢晓虹

    2012-01-01

    目的 观察滞留乳切牙与乳磨牙拔除术后止血状况.方法 选取滞留乳磨牙病儿60例,滞留乳切牙病儿60例,观察滞留乳切牙与乳磨牙拔除术后止血状况.结果 术后30 min,滞留乳切牙创口出血率低于乳磨牙组,差异有显著性(x2 =6.56,P<0.05).结论 滞留乳磨牙较滞留乳切牙拔除术后不易止血.%Objective To observe the hetnostasis after extraction of retention deciduous incisors and molars. Methods Hemostasis after tooth extraction was observed in 60 children with retention deciduous incisors and 60 with retention molars. Results Hemorrhage rate 30 min after tooth extraction was higher in molars group than the incisors group (x2=6. 56,P<0. 05). Conclusion Bleeding after extraction of retention morals is not easy to stop.

  1. How will mandibular third molar surgery affect mandibular second molar periodontal parameters?

    Science.gov (United States)

    Tabrizi, Reza; Arabion, Hamidreza; Gholami, Mehdi

    2013-07-01

    Several conflicting findings have been published in the previous literature regarding the effects of impacted third molar surgery on the periodontal parameters of the adjacent second molar; some authors have shown improvement of periodontal health distal to the adjacent second molar, whilst others have demonstrated loss of attachment level (AL) and reduction of alveolar bone height. The purpose of this study was to evaluate the changes in periodontal health parameters distal to the adjacent second molar following extraction of an impacted third molar. Out of 50 patients participated in the study, 42 patients completed the study. The mean age of the sample was 20.9 (range, 18-25) years. All teeth were mesioangular impacted mandibular third molars categorized at C1 class based on the Pell and Gregory classification. All surgeries were performed by one surgeon and the same surgeon recorded the pre-operative and post-operative measurements of probing depth (PD) and AL on the distobuccal aspect of the second molars. Data analysis were carried out with the SPSS software (version 19), using the paired-samples t-test and one sample t-test. Surgical extraction of impacted mandibular third molar resulted in a significant increase of PD on the distobuccal aspect of the second molars, whereas AL was decreased significantly after surgery (P molar after extraction of impacted third molar, our study showed a significant increase in PD at the distal aspect of the second molar. Further follow-up on clinical and radiological parameters are required for more profound understanding of the long-term effects of third molar extraction on the periodontal parameters of the adjacent second molar.

  2. A randomized, controlled, dose-ranging study investigating single doses of GW406381, naproxen sodium, or placebo in patients with acute pain after third molar tooth extraction.

    Science.gov (United States)

    Varner, Julie; Lomax, Mark; Blum, David; Quessy, Steve

    2009-09-01

    To evaluate the efficacy and tolerability of 4 doses of GW406381, a cyclooxygenase-2 inhibitor, compared with placebo in a standard model of acute inflammatory pain. This randomized, double-blind, placebo-controlled, single-center study compared single doses of GW406381 (10 to 70 mg) or naproxen sodium 550 mg with placebo in patients after extraction of 2 or more partially bony impacted third molar teeth. A total of 300 patients were randomized (50 per group). The primary efficacy variable was the pain relief intensity difference score at each time point, which was calculated as the sum of the pain intensity difference and pain relief categorical scores at each time point. Each treatment was compared with placebo at each time point using an ordered hierarchical approach with closed testing procedures and last observation carried forward imputation methods. Pain relief intensity differences from placebo were statistically significant beginning at 1.5 hours postdosing for GW406381 70 and 50 mg and at 2-hour postdosing for GW406381 25 and 10 mg. The median time to onset of analgesia was 71 minutes for GW406381 50 mg, 72 minutes for GW406381 70 mg, and 36 minutes for naproxen. The median duration of analgesia was 5.9 hours for GW406381 50 mg, 7.9 hours for GW406391 70 mg, and 11.3 hours for naproxen. All treatments were well tolerated. GW406381 50 and 70 mg demonstrated clinically meaningful analgesia in this acute pain setting, although the onset of analgesia was greater than 1 hour.

  3. Clinical effect observation of unhealthy first molar extraction in orthodontic treatment%正畸治疗并拔除病变第一磨牙的临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    钟巧芬; 马玉霞

    2011-01-01

    目的 探讨拔除病变第一恒磨牙的正畸治疗效果.方法 对16例第一磨牙严重龋坏和缺失的错(牙合)畸形患者,拔除1~3颗第一磨牙,采用直丝弓矫治技术及传统支抗技术进行矫治,做矫治结果的临床分析.结果 16例患者矫治时间16~31个月,平均22.6个月.治疗后前牙覆(牙合)覆盖正常,中线对齐,后牙尖窝交错关系,拔牙间隙关闭,牙根平行状况良好,侧貌协调.结论 在拔除第一磨牙矫治病例中,精心设计,力量合适和方法得当可获得良好的临床效果.%Objective To discuss the effectiveness of orthodontic treatment with first molar extraction. Methods Extract 1 ~ 3 first molar for malocclusion sixteen patients with unhealthy or missing first molar, treated with straight wire and traditional anchorage technique as clinical analysis of treatment effectiveness. Results The treatment duration of the sixteen patients was 16 ~ 31 months and the average duration was 22. 6 months. After treatment, anterior crossbite was corrected, normal overjet and overbite achieved,the teeth roots were paralled well, side facial appearance was good, extraction spaces were closed. Conclusion Carefully design,proper strength and methods can obtain satisfactory clinical effect in the first molar extraction case.

  4. Comparison of mineral trioxide aggregate and diluted formocresol in pulpotomized human primary molars: 42-month follow-up and survival analysis.

    Science.gov (United States)

    Mettlach, Sarah E; Zealand, Cameron M; Botero, Tatiana M; Boynton, James R; Majewski, Robert F; Hu, Jan ChingChun

    2013-01-01

    The purpose of this study was to test the hypothesis that there is no significant difference in the clinical and radiographic outcomes of diluted formocresol (DFC) compared to gray mineral trioxide aggregate (GMTA) pulpotomy in human primary molars. A total of 152 children with 252 primary molars met selection criteria. Of those, 119 and 133 teeth were randomly assigned to the GMTA and DFC groups, respectively. Periapical radiographs, taken pre- and/or postoperatively and at each 6-month follow-up, were digitized and evaluated by three blinded and calibrated examiners. Over a 42-month period, a total of 865 clinical and radiographic evaluations were conducted. There was no significant difference in clinical success, with the cumulative proportion of GMTA-treated teeth surviving at 0.98 vs DFC-treated teeth at 0.95 (P>.05). Radiographic success, however, was significantly greater for GMTA vs DFC, with the cumulative proportion of GMTA-treated teeth surviving at 0.90 vs DFC-treated teeth at 0.47 (Pformocresol when used as a medicament for primary molar pulpotomies.

  5. Calcium-enriched mixture pulpotomy of a human permanent molar with irreversible pulpitis and condensing apical periodontitis

    Directory of Open Access Journals (Sweden)

    Saeed Asgary

    2011-01-01

    Full Text Available This case report describes a pulpotomy treatment of a permanent mature molar tooth with established irreversible pulpitis and condensing apical periodontitis, using calcium-enriched mixture (CEM cement. Clinical examination revealed that the first right lower molar had a large carious lesion with history of spontaneous/lingering pain; radiographic examination showed condensing apical periodontitis. Pulpotomy was opted as the treatment; cervical pulpotomy was carried out. Following hemostasis, the radicular pulp stumps were covered with ~2-mm-thick layer of the prepared CEM cement; the tooth was restored with amalgam. Two-year follow-up showed that the treated tooth had been symptomless, and there were no clinical signs/symptoms of either inflammation or infection. Radiographically, the periradicular lesion was completely healed with reconstruction of bone structures to normal appearance; the root canals were not calcified. According to chemical, physical, and biological properties of the CEM cement, this novel biomaterial may be suitable for endodontic treatment.

  6. A Comparison between Different Coronetomy Techniques in Impacted Third Molar Extraction%不同方式分冠法拔除下颌阻生第三磨牙的临床观察

    Institute of Scientific and Technical Information of China (English)

    梁荣奇; 余飞

    2015-01-01

    Objective: To compare the clinical effects of 3 different techniques in the practice of impacted mandibular third molar surgery. Methods:210 impacted mandibular third molars (186 patients provided informed consent) were di-vided into 3 groups. Group A used “T+V” shape of cutting crown by high speed turbine handpiece, combining with“C”type of cervical increasing gap to extract the impacted mandibular third molars. Group B used “T”shape of dividing crown. Group C used dental chisel to split the impacted molar for coronectomy. The operative time length, intraoperative and postoperative complications were recorded and compared. Results: The operation time length in group A was statistically shorter than those in group B and group C (P0.05)。 A组术中断根、舌侧骨板U折断发生率较B组、C组低(P0.05)。结论:高速涡轮机配专用长裂钻或球钻进行阻生牙拔除术,采用“T+V”型分冠结合C型颈部去骨法,拔牙时间较短,术中断根率、舌侧骨板折断率较低,术后疼痛、肿胀较少。

  7. 拔除下颌第三磨牙对下颌角区形态影响的CBCT研究%Morphological Influence of the Mandibular Third Molar Extraction to the Angulusmandibulae Using Cone-Beam Computed Tomography

    Institute of Scientific and Technical Information of China (English)

    邓蓉霞; 丁寅; 孙伯阳; 郭涛; 鱼敏

    2012-01-01

    Objective: To explore the influence of the mandibular third molar extraction on the morphological changes of gonia angle. Methods: 30 adult orthodontic patients whose mandibular third molars need to be extracted for treatment had taken CBCT imaging before the teeth extracted, then 6 months later all the patients received the second CBCT scanning. We measured the width, height and length of the alveolar bone of the mandibular third molar through Mimics 10.01 software and compared the data using SPSS18.0 statistical software. Results: After the extraction of the mandibular third molar, the width and height of alveolar bone located in the gonial angle reducedand the difference had statistical significance, while the length had no conspicuous change. Conclusion: Extraction of mandibular third molar can reduce the width and height of angulusmandibulae bone to some extent, but the saggital length has no significant changes.%目的:探讨下颌第三磨牙的拔除会否造成下颌角区骨骼形态的变化以及这种变化对面型的影响.方法:30例因矫治需要拔除下颌第三磨牙的成人正畸患者,在拔牙前和拔牙后6个月拍摄CBCT片,采用三维测量软件mimics10.01测量下颌第三磨牙所在的下颌角区牙槽骨骨质的宽度、高度和长度,利用SPSS18.0统计软件分析前后数值有无差异.结果:下颌第三磨牙拔除前后下颌角区骨质宽度和高度减小,差异有统计学意义(P<0.05),下颌角区骨质前后向长度未见明显差异.结论:拔除下颌第三磨牙能从一定程度上减小下颌角区骨质高度和宽度,但是前后向长度度基本没有变化.

  8. Clinical experience of orthodontic treatment combined with extraction treatment for decayed first molar%病变第一磨牙拔除行矫治错颌畸形的临床体会

    Institute of Scientific and Technical Information of China (English)

    苟乃正; 董作英; 杨鑫

    2014-01-01

    目的:探讨拔除病变第一磨牙进行正畸治疗的可行性和矫治效果。方法:收集35例拔除病变第一磨牙同时需要进行正畸治疗的病例,平均年龄16.5岁,包括18颗上颌第一磨牙和29颗下颌第一磨牙。全部病例均采用直丝弓矫治技术,常规粘接托槽,顺序更换弓丝,待牙列排齐整平后,利用滑动法关闭拔牙间隙,精细调整咬合。结果:33例患者都达到良好的矫治效果,第一磨牙间隙完全关闭,后牙咬合关系良好,达到了改善咬合功能和美观的目的。2个陈旧性缺损病例改为修复治疗。结论:拔除龋坏第一磨牙进行错颌畸形矫治,获得良好临床疗效。%Objective:To study the feasibility and effects of orthodontic treatment combined with extraction treatment for maloc-clusion and decayed first molar. Methods:Thirty-five patients with malocclusion and decayed first molars were selected, whose aver-age age was 15. 6 years old. Eighteen upper first molars and twenty-nine lower first molars were included. All patients were treated with straight wire appliance. The procedure included bonding brackets, putting arch wire, leveling dentition, closing extraction space and adjusting occlusal relationships routinely. Results:When the treatment was finished, thirty-three cases gained satisfactory results, molar space was completely closed, occlusion was perfect, and the occlusion and facial profile were improved. However, two patients with old defects accepted prosthodontic treatment finally. Conclusions:The orthodontic treatment combined with extraction treatment for the malocclusion and decayed first molar could retain more healthy teeth, gain satisfactory orthodontic results and improve the occlu-sion.

  9. Maxillary second molar impaction in the adjacent ectopic third molar: Report of five rare cases

    Directory of Open Access Journals (Sweden)

    Bernardo Q Souki

    2015-01-01

    Full Text Available Maxillary second molar impaction in the adjacent ectopic third molar is a rare condition that practitioners might face in the field of pediatric dentistry and orthodontics. The early diagnosis and extraction of the adjacent ectopic third molar have been advocated, and prior research has reported a high rate of spontaneous eruption following third molar removal. However, some challenges in the daily practice are that the early diagnosis of this type of tooth impaction is difficult with conventional radiographic examination, and sometimes the early surgical removal of the maxillary third molar must be postponed because of the risks of damaging the second molar. The objective of this study is to report a case series of five young patients with maxillary second molar impaction and to discuss the difficulty of early diagnosis with the conventional radiographic examination, and unpredictability of self-correction.

  10. Comparison of buccal infiltration of 4% articaine with 1 : 100,000 and 1 : 200,000 epinephrine for extraction of maxillary third molars with pericoronitis: a pilot study.

    Science.gov (United States)

    Lima, José Lacet; Dias-Ribeiro, Eduardo; Ferreira-Rocha, Julierme; Soares, Ramon; Costa, Fábio Wildson Gurgel; Fan, Song; Sant'ana, Eduardo

    2013-01-01

    We compared the buccal infiltration of 4% articaine with 1 : 100,000 or 1 : 200,000 epinephrine without a palatal injection for the extraction of impacted maxillary third molars with chronic pericoronitis. This prospective, double-blind, controlled clinical trial involved 30 patients between the ages of 15 and 46 years who desired extraction of a partially impacted upper third molar with pericoronitis. Group 1 (15 patients) received 4% articaine with 1 : 100,000 epinephrine and group 2 (15 patients) received 4% articaine with 1 : 200,000 epinephrine by buccal infiltration. None of the patients in group 1 reported pain, but 3 patients in group 2 reported pain, which indicated a need for a supplementary palatal injection. The palatal injections were all successful in eliminating the pain. Two additional patients in group 2 experienced pain when the suture needle penetrated their palatal mucosa. Based on these results, 4% articaine with 1 : 100,000 epinephrine was found to be more effective for the removal of upper third molars in the presence of pericoronitis than 4% articaine hydrochloride with 1 : 200,000 epinephrine when only a buccal infiltration was used.

  11. Human dental age estimation using third molar developmental stages: Accuracy of age predictions not using country specific information.

    Science.gov (United States)

    Thevissen, P W; Alqerban, A; Asaumi, J; Kahveci, F; Kaur, J; Kim, Y K; Pittayapat, P; Van Vlierberghe, M; Zhang, Y; Fieuws, S; Willems, G

    2010-09-10

    Unquestionable forensic age investigations are based on statistical models constructed on a sample containing subjects of identical origin as the examined individual. In cases where corresponding models are unavailable, the established report has to describe the possible effects of this unrelated information on the predicted age outcome. The aim of this study is to collect country specific databases of third molar development and to verify how the related dental age estimations are influenced if we were to use dental developmental information only from Belgium or from all collected countries together. Data containing third molar developmental stages scored following Gleiser and Hunt (modified by Köhler) were collected from 9 country specific populations (Belgium, China, Japan, Korea, Poland, Thailand, Turkey, Saudi-Arabia and South-India). Age predictions were obtained from a training dataset and validated on a test dataset. Bayes rule using the repeated third molar scores is applied to get age predictions and prediction intervals. Three age predictions were compared for males and females separately. For the first prediction, the training dataset contains only Belgian subjects. For the second prediction, the training dataset for each country consists only of subjects of the country itself. For the final prediction, subjects from all countries are pooled into one common training dataset. Besides the (absolute) difference between the chronological age and the predicted age, specific interest lies in the juvenile-adult distinction. In the age range from 16 to 22 years 6982 subjects (3189 male and 3793 female) were analyzed. Using information on third molar development from Belgium compared to information from the country specific databases hardly increased the mean absolute differences (MAD) and mean squared errors (MSE): the MAD and MSE increased on average with 0.5 and 2.5 months with maximal increases of, respectively 1.6 and 7.3 months. Using information from all

  12. The iontophoresis of lignocaine with epinephrine into carious dentine for pain control during cavity preparation in human molars.

    Science.gov (United States)

    Smitayothin, The Late Korporn; Vongsavan, Kadkao; Rirattanapong, Praphasri; Kraivaphan, Petcharat; Vongsavan, Noppakun; Matthews, Bruce

    2015-08-01

    To determine the effectiveness of the iontophoretic delivery of lignocaine with epinephrine through carious dentine for pain control during cavity preparation. The experiments were carried out on 56 carious molars that required class I restorations in 42 subjects (aged 15-20 years). The overhanging enamel and soft caries were removed then the sensitivity of the exposed dentine was tested with drilling, probing and air blast stimuli. The subject indicated the intensity of any pain produced by marking a visual analogue scale (VAS). The cavity was then filled with 20% w/v lidocaine with 0.1% w/v epinephrine and a 200 μA iontophoretic current applied for 2 min after which the sensitivity of the dentine was re-tested. If the dentine was not anaesthetized, the treatment and testing were repeated up to 6 times. The total duration (min) of iontophoresis required to anaesthetize the dentine was: 2 in 7 teeth, 4 in 17 teeth, 6 in 14 teeth, 8 in 4 teeth, and 10 in 7 teeth. The remaining 7 teeth were not anaesthetized even after 14 min of iontophoresis. The iontophoretic delivery of lignocaine with epinephrine anaesthetized dentine for cavity preparation in 49 of 56 (87.5%) of carious molars. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Positional changes of the third molar in orthodontically treated patients

    OpenAIRE

    2013-01-01

    Objective and Rationale. Over the years, the effects of the third molars eruption on the dental arches have been studied extensively. Still, literature provides less data regarding the effects of the orthodontic treatment on the third molars position. The aim of our study was to assess the positional changes of the third molars relative to the occlusal plane and to the second molar long axis, changes occurred during orthodontic treatment performed with or without premolar extractions. Method....

  14. Deciduous molar hypomineralization and molar incisor hypomineralization.

    Science.gov (United States)

    Elfrink, M E C; ten Cate, J M; Jaddoe, V W V; Hofman, A; Moll, H A; Veerkamp, J S J

    2012-06-01

    This study was embedded in the Generation R Study, a population-based prospective cohort study from fetal life until young adulthood. This study focused on the relationship between Deciduous Molar Hypomineralization (DMH) and Molar Incisor Hypomineralization (MIH). First permanent molars develop during a period similar to that of second primary molars, with possible comparable risk factors for hypomineralization. Children with DMH have a greater risk of developing MIH. Clinical photographs of clean, moist teeth were taken with an intra-oral camera in 6,161 children (49.8% girls; mean age 74.3 mos, SD ± 5.8). First permanent molars and second primary molars were scored with respect to DMH or MIH. The prevalence of DMH and MIH was 9.0% and 8.7% at child level, and 4.0% and 5.4% at tooth level. The Odds Ratio for MIH based on DMH was 4.4 (95% CI, 3.1-6.4). The relationship between the occurrence of DMH and MIH suggests a shared cause and indicates that, clinically, DMH can be used as a predictor for MIH.

  15. Regenerative Endodontic Treatment of an Immature Necrotic Molar with Arrested Root Development by Using Recombinant Human Platelet-derived Growth Factor: A Case Report.

    Science.gov (United States)

    Zhujiang, Annie; Kim, Sahng G

    2016-01-01

    Regenerative endodontic treatment has provided a treatment option that aims to allow root maturation. The present report describes the regenerative endodontic treatment of a necrotic, immature molar by using recombinant human platelet-derived growth factor (rhPDGF-BB) and shows the continued root maturation in the tooth with arrested root development. A regenerative endodontic procedure that used a growth factor was performed for a necrotic molar with arrested root formation in a 20-year-old patient. Thorough disinfection by using mechanical instrumentation and copious irrigation of antimicrobial agents as well as intracanal medication with calcium hydroxide was performed throughout the first 2 appointments. At the third appointment, the root canals were irrigated with an antimicrobial solution and 17% EDTA, and bleeding was evoked by passing sterile paper points beyond the apex in each canal. Small pieces of a collagen membrane saturated with rhPDGF-BB solution from GEM 21S were packed into each canal. Mineral trioxide aggregate was placed, and Cavit and composite resin were used to restore the tooth. Complete root maturation and resolution of a periapical radiolucency were observed at the 15-month follow-up. The present report presents a regenerative endodontic procedure that uses rhPDGF-BB for a necrotic molar with arrested root development. The finding of continued root development in the present case suggests that regenerative endodontic treatment may be able to resume the root maturation process in teeth with arrested root formation. Further clinical studies are required to investigate the efficacy of rhPDGF-BB in regenerative endodontic treatment. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  16. Comparative Study of Pulp Vitality in Primary and Young Permanent Molars in Human Children with Pulse Oximeter and Electric Pulp Tester

    Science.gov (United States)

    Sood, PB; Sharma, Arun; Madan, Manish; Shahi, Nishat; Gandhi, Geetanjali

    2015-01-01

    ABSTRACT Aim and objective: The purpose of this study was to compare the pulp testing methods (pulse oximetry and electric pulp test) in primary and young permanent teeth of children. Materials and methods: The study included a total of 155 children aged 4 to 15 years. Twenty children formed control group I. Study group included all healthy, 85 primary 2nd molars in group II and 85 permanent 1st molars in group III. Fifty children needing endodontics treatment formed test group IV. The readings were recorded as true positive (TP), false positive (FP), true negative (TN), false negative (FN). Based on this, the sensitivity, specificity, positive predictive value and negative predictive value were calculated for each method. The results were statistically analyzed using Chi-square test. Results: On comparing pulse oximetry with electric pulp test ‘p-value’ was found to be 0.487 and 1.00 for groups 1 and 2 respectively and was statistically not significant. Whereas ‘p-value’ for groups 3 and 4 was < 0.0001 and 0.003 respectively and was statistically highly significant. Conclusion: The present study indicates that pulse oximetry can be used as a routine method for assessing the pulp vitality in primary, young permanent and mature permanent teeth. How to cite this article: Shahi P, Sood PB, Sharma A, Madan M, Shahi N, Gandhi G. Comparative Study of Pulp Vitality in Primary and Young Permanent Molars in Human Children with Pulse Oximeter and Electric Pulp Tester. Int J Clin Pediatr Dent 2015;8(2):94-98. PMID:26379374

  17. Antibacterial activity of mangrove leaf extracts against human pathogens

    Digital Repository Service at National Institute of Oceanography (India)

    Sahoo, G.; Mulla, N.S.S.; Ansari, Z.A.; Mohandass, C.

    The antibacterial activity of leaf extract of mangroves, namely, Rhizophora mucronata, Sonneratia alba and Exoecaria agallocha from Chorao island, Goa was investigated against human bacterial pathogens Staphylococcus aureus, Streptococcus sp...

  18. Evaluation of CGF on the Quality of Life after Impacted Lower Third Molar Extraction%CGF对下颌阻生第三磨牙拔除术后生活质量的影响

    Institute of Scientific and Technical Information of China (English)

    龚仁国; 阙佳佳; 肖海鹰; 陈茜

    2016-01-01

    Objective To evaluate the impaction of CGF on the quality of life after lower impacted third molar extraction. Methods 100 patients with impacted third molar were divided into two groups at random with 50 in each group. In group 1 the CGF was put into the socket after tooth extraction. Group 2 was the black control. PoSSe scale was used to evaluate the quality of life after treatment. Results CGF score of PoSSe group was lower than that of control group, the difference was statistically significant (P<0.05). Conclusions CGF can improve the quality of life after lower third molar extraction.%目的:评价浓缩生长因子(CGF)对下颌阻生第三磨牙拔除术后患者生活质量的影响。方法选择2014年9月~2015年10月到我院接受拔除单颗下颌中位阻生第三磨牙的100例患者,将其随机分为CGF组与对照组,拔牙后CGF组向牙槽窝中置入CGF,对照组为空白对照,应用术后症状严重度量表(PoSSe)对患者术后生活质量进行综合评价。结果 CGF组的PoSSe分值低于对照组,差异有统计学意义(P<0.05)。结论 CGF可有效提高下颌阻生第三磨牙拔除术后患者的生活质量。

  19. Pulpectomy procedures in primary molar teeth

    Directory of Open Access Journals (Sweden)

    Hany Mohamed Aly Ahmed

    2014-01-01

    Full Text Available Premature loss of primary molars can cause a number of undesirable consequences including loss of arch length, insufficient space for erupting premolars and mesial tipping of the permanent molars. Pulpectomy of primary molar teeth is considered as a reasonable treatment approach to ensure either normal shedding or a long-term survival in instances of retention. Despite being a more conservative treatment option than extraction, efficient pulpectomy of bizarre and tortuous root canals encased in roots programmed for physiologic resorption that show close proximity to developing permanent tooth buds presents a critical endodontic challenge. This article aims to provide an overview of this treatment approach, including partial and total pulpectomy, in primary molar teeth. In addition, the recommended guidelines that should be followed, and the current updates that have been developed, while commencing total pulpectomy in primary molars are discussed.

  20. 下颌水平阻生智齿拔牙术的护理配合%The Care with Mandibular Horizontal Third Molar Extraction Surgery

    Institute of Scientific and Technical Information of China (English)

    范萍

    2014-01-01

    随着人类咀嚼器官的逐渐退化,阻生智齿的发生率越来越多,而下颌智齿阻生的发生率较高,并常引起冠周炎、邻牙龋坏、间隙感染及骨髓炎等并发症,因此常需拔除。%With the gradual degradation of the human masticating apparatus, the incidence of impacted wisdom teeth is increasing,and mandible impacted wisdom teeth have a higher rate,it often cause some complications like pericoronitis,caries of adjacent teeth,space infections and osteomyelitis,so the wisdom teeth usual y require to be extracted.

  1. 拔除智齿矫治成人Ⅲ类骨性错(牙合)1例%Treatment for adult skeletal class Ⅲ malocclusion by the extraction of the 4 third molars: A case report

    Institute of Scientific and Technical Information of China (English)

    郭丛丛; 包柏成

    2012-01-01

    通过拔除第三磨牙,利用第二磨牙远中与上颌结节后缘/下领升支前缘之间的剩余间隙,最终解除了上牙弓的Ⅱ度拥挤及上下牙弓矢状关系的不调.%By extraction of the 4 third molars, the spaces from the posterior dental arch were available to relieve moderate maxillary crowding and correct Class HI malocclusion, effective treatment was carried out in 1 ease.

  2. Efficacy of low-level laser therapy in the management of pain, facial swelling, and postoperative trismus after a lower third molar extraction. A preliminary study.

    Science.gov (United States)

    López-Ramírez, Marta; Vílchez-Pérez, Miguel Angel; Gargallo-Albiol, Jordi; Arnabat-Domínguez, Josep; Gay-Escoda, Cosme

    2012-05-01

    Pain, swelling, and trismus are the most common complications after surgical removal of impacted lower third molars. The aim of this study was to evaluate the analgesic and anti-inflammatory effects of a low-level laser therapy (Laser Smile™, Biolase®, San Clemente, USA) applied to the wound appeared after the surgical removal of impacted lower third molars. A prospective, randomized, and double-blind study was undertaken in 20 healthy patients with two symmetrically impacted lower third molars. The application of a low-level laser was made randomly on one of the two sides after surgery. The experimental side received 5 J/cm(2) of energy density, a wavelength of 810 nm, and an output power of 0.5 W. On the control side, a handpiece was applied intraorally, but the laser was not activated. Evaluations of postoperative pain, trismus, and swelling were made. The sample consisted of 11 women and nine men, and mean age was 23.35 years (18-37). The pain level in the first hours after surgery was lower in the experimental side than in the placebo side, although without statistically significant differences (p = 0.258). Swelling and trismus at the 2nd and 7th postoperative days were slightly higher in the control side, although not statistically significant differences were detected (p > 0.05). The application of a low-level laser with the parameters used in this study did not show beneficial affects in reducing pain, swelling, and trismus after removal of impacted lower third molars.

  3. Diagnostic accuracy of cone-beam CT compared with panoramic images in predicting retromolar canal during extraction of impacted mandibular third molars

    OpenAIRE

    Sisman, Yildiray; Sekerci, Ahmet Ercan; Payveren Arıkan, Mehtap; Sahman, Halil

    2015-01-01

    Objectives: The clinical significance of the existence of a retromolar canal and of its neurovascular content is not yet clear.The aim of the present study was to assess the visibility, diameter and course of the mandibular retromolarcanal (MRC) using cone beam computed tomography (CBCT) scan -had been taken for pre-operative radiographic evaluation of impacted mandibular third molars- compared to panoramic radiographs. Study Design: Subjects eligible for study enrollment were those who under...

  4. The effect of cigarette smoking on the severity of pain, swelling and trismus after the surgical extraction of impacted mandibular third molar

    Science.gov (United States)

    2013-01-01

    Objective: The study objective was to investigate the effect of cigarette smoking on the severity of pain, swelling and trismus on male after the surgical removal of impacted lower third molar. Material and Methods: This prospective comparative study was conducted for 150 male in two groups of patients, smokers and non-smokers. Each group consisted of 75 patients; smoking patient were the ones who smoke more than twenty cigarettes per day for more than one year of continuous smoking. Postoperative pain was evaluated using a visual analog scale (VAS) and the degree of swelling was evaluated through facial reference points’ variation. The presence of trismus was analyzed through measurement of the interincisal distance (IID). Result: Clinical and radiographic examinations were carried out. Data regarding the age, gender, angulations type, depth and width of impactions were evaluated and analyzed The severity of pain, swelling and trismus on the 1st, 2nd , 5th and 7thday postoperatively was estimated. In both groups the pain and trismus were reported to be in peak level during the first post-operative day while post-operative swelling reaches its peak level in the second postoperative day. Conclusion: Cigarettes smoking do not have any significant relationship with the severity of pain, swelling and trismus after surgical removal of lower third molar on male gender. Key words:Cigarettes smoking, pain, swelling, trismus, impacted lower third molars. PMID:24455065

  5. Effect of the local administration of betamethasone on pain, swelling and trismus after impacted lower third molar extraction. A randomized, triple blinded, controlled trial.

    Science.gov (United States)

    Marques, José; Pié-Sánchez, Jordi; Figueiredo, Rui; Valmaseda-Castellón, Eduard; Gay-Escoda, Cosme

    2014-01-01

    The aim of this study is to compare the analgesic and anti-inflammatory effects of the local postoperative administration of a single 12-mg dose of betamethasone after the surgical removal of impacted lower third molars. A split-mouth, triple-blind, randomized, placebo-controlled clinical trial of 25 patients requiring the surgical removal of symmetrical lower third molars was performed. In the experimental side, a 12-mg dose of betamethasone was administered submucosally after the surgical procedure, while in the control side a placebo (sterile saline solution) was injected in the same area. To assess postoperative pain, visual analogue scales and the consumption of rescue analgesic were used. The facial swelling and trismus were evaluated by measuring facial reference distances and maximum mouth opening. There were no significant differences between the two study groups regarding postoperative pain, facial swelling and trismus. The injection of a single dose of betamethasone does not seem to reduce pain, facial swelling and trismus after impacted lower third molar removal when compared to placebo.

  6. Sextant of Sapphires for Molar Distalization

    Science.gov (United States)

    Palla, Yudistar Venkata; Ganugapanta, Vivek Reddy

    2016-01-01

    Introduction Space analysis quantifies the amount of crowding within the arches estimating the severity of space discrepancy. The space gaining procedures include extraction and non-extraction procedures like expansion, proximal stripping and molar distalization. Aim To identify features seen in molar distalization cases. Materials and Methods The sample size comprised 20 patients in whom molar distalization was decided as the treatment plan. The study models and lateral cephalograms of all the patients were taken. Occlusograms were obtained. Model analysis and cephalometric analysis were performed. Descriptive statistical analysis like mean, standard deviation, standard error and mode were done. Results The parameters in Question gave following results. The Bolton analysis showed anterior mandibular excess with mean value of 1.56mm±1.07. The first order discrepancy between maxillary central and lateral incisors was 5±1.95. The premolar rotation showed mean value of 16.58±5.12. The molar rotation showed the value of 7.66±2.26. The nasolabial angle showed the mean of 101.25±8.7 IMPA of 101.4±5.74. Conclusion The six features studied in molar distalization cases [First order discrepancy between upper central and lateral incisors; Rotation of premolars and molars; Bolton’s discrepancy in anterior dentition; Average to horizontal growth pattern; Proclined lower incisors and Obtuse nasolabial angle] can be taken as patterns seen in molar distalization cases and considered as a valid treatment plan. PMID:27656572

  7. Third molars: A review

    Directory of Open Access Journals (Sweden)

    E Siva Prasad Reddy

    2009-01-01

    Full Text Available Background: Third molars are teeth that have little functional value and a relatively high rate of associated pain and disease. Their value, as part of the dentition of modern people, is dubious. Types of Studies Reviewed: The authors review the evolution, development. moribidity and treatment of third molars. They access the value of third molars in the 21st century and describe the risks these teeth pose when they develop in the dentition. Conclusions: There is a mandate for the dental profession to improve health outcomes and quality of life. The prevention of third molar-related morbidity should be included in dental research efforts. The authors suggest that novel preventive methodologies be developed to alleviate the problems third molars pose. One potential methodology suggested is intentional therapeutic agenesis of this tooth. Clinical Implications: Prevention of third molar development early in life, even before tooth bud initiation, could dramatically improve healthcare outcomes for millions of peoples.

  8. Third molars: A review

    OpenAIRE

    E Siva Prasad Reddy; Y Narendranath Reddy

    2009-01-01

    Background: Third molars are teeth that have little functional value and a relatively high rate of associated pain and disease. Their value, as part of the dentition of modern people, is dubious. Types of Studies Reviewed: The authors review the evolution, development. moribidity and treatment of third molars. They access the value of third molars in the 21st century and describe the risks these teeth pose when they develop in the dentition. Conclusions: There is a mandate for the dental prof...

  9. Removal of Deeply Impacted Mandibular Molars by Sagittal Split Osteotomy

    Directory of Open Access Journals (Sweden)

    Erol Cansiz

    2016-01-01

    Full Text Available Mandibular third molars are the most common impacted teeth. Mandibular first and second molars do not share the same frequency of occurrence. In rare cases the occlusal surfaces of impacted molars are united by the same follicular space and the roots pointing in opposite direction; these are called kissing molars. In some cases, a supernumerary fourth molar can be seen as unerupted and, in this case, such a supernumerary, deeply impacted fourth molar is seen neighboring kissing molars. The extraction of deeply impacted wisdom molars from the mandible may necessitate excessive bone removal and it causes complications such as damage to the inferior alveolar nerve and iatrogenic fractures of the mandible. This case report describes the use of the sagittal split osteotomy technique to avoid extensive bone removal and protect the inferior alveolar nerve during surgical extruction of multiple impacted teeth.

  10. The apo E/apo CIII molar ratio affects removal of cholesterol ester from modified human lipoproteins injected into cebus monkeys.

    Science.gov (United States)

    Stephan, Z F; Gibson, J C; Hayes, K C

    1986-04-14

    The removal of postprandial (PP) and postabsorptive (PA) human LDL and HDL cholesterol was examined in cebus monkeys (Cebus albifrons) following in vitro labelling of these lipoproteins by 3H-cholesterol in the presence or absence of DTNB. The removal of LDL cholesteryl ester was 3.5 and 2 times greater than that of HDL in male and female monkeys, respectively. Incubation with DTNB reduced cholesteryl ester removal by 45 and 52% for LDL and HDL, respectively. Cholesteryl ester from PA lipoproteins was removed 80% faster than that PP particles only when plasma was incubated without DTNB. Cholesterol removal from these lipoproteins was positively (r = 0.941) and significantly (P less than 0.001) correlated with the molar apo E/apo CIII ratio. The data suggest that density of lipoproteins was less important than their apoprotein composition in dictating their removal from circulation.

  11. Local vs general anaesthesia in the development of neurosensory disturbances after mandibular third molars extraction: A retrospective study of 534 cases

    Science.gov (United States)

    Biasotto, Matteo; Maglione, Michele; Di Lenarda, Roberto

    2016-01-01

    Background The choice of the anaesthetic modality is one of the primary steps during planning of third molar surgery. The aim of the present study was to compare the risk of developing neurological injures of the inferior alveolar nerve (IAN) and lingual nerve (LN) in patients treated for wisdom teeth removal under general anaesthesia (GA) with a group treated under local anaesthesia (LA). Material and Methods This is an observational retrospective, unicentric study; between September 2013 and September 2014, 534 patients underwent third molar surgery, 194 (36,3%) under GA and 340 (63,7%) under LA by the same oral surgeon. Differences in the incidence of IAN and LN injures between groups have been statistically analyzed with Fisher exact test and estimated odd ratio for development of such complications has been calculated. Results In GA patients the incidence of IAN and LN injures was 4.6% and 2.1%, respectively while in the LA group it was and 0.3% and 0%, respectively. A significant difference in IAN and LN involvement between groups was observed (IAN lesion: Fisher exact test, p<0.001; LN lesions: Fisher exact test, p<0.05). The estimated odd ratio for development of IAN injures after GA was 16.49 (95% CI: 2.07-131.19) and was not calculable for LN injures because no cases were observed in the LA group. Conclusions Since GA is a perioperative variable that seems to significantly increase the risk of developing IAN and LN lesions, when treating patients that request GA, they must be adequately informed that an higher incidence of post-surgical sensory disturbances is expected. Key words:Third molars, general anaesthesia, local anaesthesia, inferior alveolar nerve, lingual nerve. PMID:27694783

  12. Inflammatory process decrease by gallium-aluminium-arsenide (GaAlAs) low intensity laser irradiation on postoperative extraction of impacted lower third molar; Reducao de processo inflamatorio com aplicacao de laser de arseneto de galio aluminio ({lambda}=830 nm) em pos-operatorio de exodontia de terceiros molares inferiores inclusos ou semi-inclusos

    Energy Technology Data Exchange (ETDEWEB)

    Atihe, Mauricio Martins

    2002-07-01

    This study aimed the observation of inflammatory process decrease by the use of GaAlAs Low Intensity Laser ({lambda}=830 nm; 40 mW) irradiation. Five patients were selected and submitted to surgery of impacted lower third molars, both right and left sides at different occasions. On a first stage, a tooth of a random chosen side - right or left - was extracted by conventional surgery, without LILT. The inflammatory process was measured at postoperative on the first, third and seventh days. This side was then called 'control side'. After 21 days, period in which the inflammatory process of the first surgery was terminated, the other side surgery took place, this time using LILT (4 J at four spots) at postoperative, first and third days. As the previous surgery, the inflammatory process was also measured at postoperative on the first, third and seventh days. This side was called 'experimental or lased side'. The inflammatory process was evaluated by measuring its four characteristic signs: swelling, pain, color and temperature. It was clearly observed a decrease for swelling, pain and color on the lased side which presented significant inference and descriptive statistics. It can be concluded that GaAlAs Low Intensity Laser ({lambda}=830 nm) can surely be used as an additional and important anti-inflammatory source on impacted lower third molar surgeries. (author)

  13. Silhouette extraction from human gait images sequence using cosegmentation

    Science.gov (United States)

    Chen, Jinyan; Zhang, Yi

    2012-11-01

    Gait based human identification is very useful for automatic person recognize through visual surveillance and has attracted more and more researchers. A key step in gait based human identification is to extract human silhouette from images sequence. Current silhouette extraction methods are mainly based on simple color subtraction. These methods have a very poor performance when the color of some body parts is similar to the background. In this paper a cosegmentation based human silhouette extraction method is proposed. Cosegmentation is typically defined as the task of jointly segmenting "something similar" in a given set of images. We can divide the human gait images sequence into several step cycles and every step cycle consist of 10-15 frames. The frames in human gait images sequence have following similarity: every frame is similar to the next or previous frame; every frame is similar to the corresponding frame in the next or previous step cycle; every pixel can find similar pixel in other frames. The progress of cosegmentation based human silhouette extraction can be described as follows: Initially only points which have high contrast to background are used as foreground kernel points, the points in the background are used as background kernel points, then points similar to foreground points will be added to foreground points set and the points similar to background points will be added to background points set. The definition of the similarity consider the context of the point. Experimental result shows that our method has a better performance comparing to traditional human silhouette extraction methods. Keywords: Human gait

  14. PENARIKAN GIGI MOLAR IMPAKSI PADA RAHANG BAWAH (Laporan Kasus

    Directory of Open Access Journals (Sweden)

    Debora Hidayat

    2015-08-01

    Full Text Available Impacted mandibular molars often caused by locking of the adjacent teeth, lack of space and many other reasons. Surgical extraction used to be the first choice in treating the severely impacted molars. In this article, firstly a horizontally impacted mandibular first molar and a mandibular second molar were diagnosed radiographically. By surgical crown exposure, combined with elastic traction, the teeth can be pulled occlusally into proper position. However, a thorough observation to control the position of the impacted molars during traction is still necessary.

  15. Displacement of maxillary third molar into the lateral pharyngeal space.

    Science.gov (United States)

    Lee, Doksa; Ishii, Syoichiro; Yakushiji, Noboru

    2013-10-01

    Iatrogenic tooth displacement is a rare complication during extraction of impacted molars, but displacement of a maxillary third molar into the maxillary sinus, infratemporal fossa, buccal space, pterygomandibular space, and lateral pharyngeal space has been reported. Currently, 6 published reports describe third molar displacement into the lateral pharyngeal space, only 1 of which involved the loss of a maxillary third molar into this area, which occurred after an attempted self-extraction by the patient. There have been no reported cases of iatrogenic displacement of the maxillary third molar during an extraction procedure. This article describes the recovery, under general anesthesia, of a maxillary third molar from the lateral pharyngeal space after an iatrogenic displacement.

  16. Influence of treatment including second molars on final and postretention molar angulation

    Directory of Open Access Journals (Sweden)

    Luiz Filiphe Gonçalves Canuto

    2013-10-01

    Full Text Available OBJECTIVE: Evaluate axial mesiodistal inclinations of the mandibular molars in orthodontically treated cases, analyzing whether inclusion of second mandibular molars in treatment mechanics has any influence on final and postretention molars angulations. METHODS: The sample comprised 150 panoramic radiographs of 50 patients. Patients were treated with extraction of four first premolars and divided into 2 groups: Group 1 comprised 25 subjects without inclusion of mandibular second molars during orthodontic treatment, whereas Group 2 comprised 25 subjects with inclusion of mandibular second molars. Panoramic radiographs at three observation times were evaluated: pretreatment, posttreatment and postretention. The statistical analysis included one-way analysis of variance (ANOVA for intragroup evaluation and independent t-tests for intergroup comparisons. RESULTS: Intragroup analysis demonstrated significant uprighting of mandibular first and second molars during treatment in Group 2, which remained stable during the postretention stage. Intergroup comparison demonstrated that Group 2 presented first and second molars significantly more uprighted in relation to Group 1 in both post-treatment and postretention stages. CONCLUSIONS: It was concluded that inclusion of mandibular second molars in the orthodontic mechanics is relevant not only to correct the angulation of these teeth, but also to aid mandibular first molars uprighting.

  17. Corticoesteroides y cirugía del tercer molar inferior: Revisión de la literatura Corticoesteroids and impacted lower third molar surgery: Literature review

    Directory of Open Access Journals (Sweden)

    I Prieto

    2005-10-01

    Full Text Available El objetivo de este trabajo es buscar la evidencia disponible con respecto al uso, justificación, efectos secundarios y eficacia de corticosteroides para reducir el dolor e inflamación postoperatorios en la extracción quirúrgica de terceros molares. Queremos saber si en pacientes adultos, es la prescripción de antiinflamatorios esteroideos además de los antiinflamatorios no esteroideos (AINES, en comparación con la administración única de AINES, más efectiva para mejorar, aliviar o eliminar los síntomas de inflamación y dolor después de la extracción quirúrgica de terceros molares inferiores. En la revisión de la literatura se ha utilizando la base de datos Medline con las palabras clave "esteroides" y "terceros molares" o "Muelas del juicio" y "Cirugía". La búsqueda fue limitada también a estudios en humanos, lengua inglesa y revistas dentales.The aim of this study is searching the available evidence with respect to the use, justification, side effects and efficacy of corticosteroids in reducing pain and swelling after the surgical extraction of impacted lower third molars. We want to investigate if steroidal anti-inflammatories prescription in addition to non-steroidal anti-inflammatory drugs (NSAID, when compared with the exclusive administration of NSAID, is more effective improving, relieving or eradicating symptoms of swelling and pain after the extraction of the impacted lower third molar. We have utilize in this literature review the Medline database with the keywords "steroids" and "Third molars" or "wisdom molars" and "surgery". The search was also limited to humans, English and dental journals.

  18. Minimally invasive surgery versus chisel osteotomy in the extraction of impacted mandibular third molars%微创法与锤凿法拔除下颌阻生智齿的临床研究

    Institute of Scientific and Technical Information of China (English)

    肖平; 卢向阳; 余尚龙; 吕美玲; 陈芝

    2012-01-01

    Objective: To assess the application value of minimally invasive surgery (MIS) in the extraction of impacted mandibular third molars. Methods: A total of 342 patients with impacted mandibular third molars to be extracted were randomly assigned to an experimental group and a control group to be performed tooth extraction by MIS and traditional chisel osteotomy, respectively. We compared the two groups in the operation time and postoperative complications, such as swelling, pain, limitation of mouth opening, sore temporomandibular joint and dry socket. Results: Compared with the controls, the experimental group showed significantly shorter operation time (P<0.001), lower rates of postoperative pain, swelling, limitation of mouth opening, dry socket and sore temporomandibular joint (P<0.005). Conclusion: MIS, with its evident superiority to chisel osteotomy, would gradually take place of the latter in the extraction of impacted mandibular third molars.%目的:评估微创拔牙技术在阻生牙拔除术中的应用价值.方法:选择342例需拔除下颌阻生智齿患者,随机分成2组,分别采用微创法和锤凿法拔除患牙,记录两种方法平均拔除时间、术后肿胀、疼痛、张口受限、颞颌关节痛、干槽症等并发症发生情况.结果:实验组与对照组相比,平均拔除时间明显缩短(P<0.001);术后疼痛、肿胀、张口受限、干槽症、颞颌关节痛等并发症发生率较对照组明显降低(P<0.005).结论:随着微创医学的发展及新型拔牙器械的推出,微创拔牙理念和技术将逐渐取代传统的锤凿法.

  19. Analysis of the therapeutic effects of orthodontic therapy with mandibular first molar extraction%拔除下颌第一磨牙矫治错牙合畸形的疗效分析

    Institute of Scientific and Technical Information of China (English)

    吴颖; 彭国光; 朱双林

    2014-01-01

    目的:评价拔除下颌第一磨牙治疗错!畸形的疗效。方法:根据拔牙方式将77例错!畸形患者分为常规拔除第一前磨牙组30例、单侧拔除第一磨牙组27例和双侧拔除第一磨牙组20例;测量矫治前后OGS各指数变化情况并进行统计学分析。结果:3组正畸治疗后牙!均有显著的改善(P<0.01),但3组的改变量无明显差异(P>0.05);3组正畸后OGS指数有显著差异(P<0.05),双侧拔除第一前磨牙组优于双侧拔除第一磨牙组(P<0.01),双侧拔除第一磨牙组优于单侧拔除第一磨牙组(P<0.01)。结论:拔除第一磨牙矫治能取得较好的矫治效果,但拔除第一前磨牙在后牙!的矫治中效果更好。%Objective:To evaluate the therapeutic effects of orthodontic therapy with mandibular first molar extraction.Methods:77 cases of malocclusion were divided into 3 groups:Bilateral mandibular first premolar extraction group(A,30 cases),unilateral man-dibular first molar extraction group(B,27cases)and bilateral mandibular first molar extraction group(C,20 cases).The OGS indexes were measured before and after orthodontic treatment and statistically analysed.Results:Remarkable occlusal improvement was seen in all three groups after treatment(P0.05).OGS indexes among the three groups after treatment were significantly different(P<0.05).The improvement in group A was more significant than that in group C(P<0.01)and the improvement in group C was more significant than that in group B(P<0.01). Conclusion:Orthodontic therapy with bilateral mandibular first molar extraction is effective in orthodontic treatment of malocclusion, but is not as effective as that with bilateral mandibular first premolar extraction.

  20. Fusión de un tercer molar mandibular con un cuarto molar supernumerario Fusion of mandibular third molar with supernumerary fourth molar

    Directory of Open Access Journals (Sweden)

    C. López Carriches

    2008-10-01

    Full Text Available La fusión dental es la unión de dos gérmenes dentales normalmente separados, mientras que la geminación se define como el intento de división de un único germen dental. La fusión y geminación de molares es poco frecuente en la dentición permanente. Describimos un caso clínico de un tercer molar inferior derecho fusionado a un cuarto molar supernumerario en un paciente varón de 36 años que ha presentado repetidos episodios de pericoronaritis. Tras el estudio radiológico se realiza la exodoncia del cordal semiincluido bajo anestesia local. Llevamos a cabo una revisión bibliográfica al respecto.Dental fusion is the union of two tooth buds that normally are separated, while gemination is defined as an attempt by a single tooth bud to divide. The fusion and gemination of molars is uncommon in permanent teeth. We report a clinical case of a right lower third molar fused to a supernumerary fourth molar in a 36-year-old male patient with repeated episodes of inflammation. After the radiologic study, the semi-impacted third molar was extracted under local anesthesia. The literature was reviewed.

  1. Inflammation and wound healing in cats with chronic gingivitis/stomatitis after extraction of all premolars and molars were not affected by feeding of two diets with different omega-6/omega-3 polyunsaturated fatty acid ratios.

    Science.gov (United States)

    Corbee, R J; Booij-Vrieling, H E; van de Lest, C H A; Penning, L C; Tryfonidou, M A; Riemers, F M; Hazewinkel, H A W

    2012-08-01

    Feline chronic gingivitis/stomatitis (FCGS) is a painful inflammatory disease in cats. Extraction of teeth, including all premolars and molars, has been shown to be the therapy of choice in cats not responding sufficiently to home care (e.g. tooth brushing) and/or medical treatment (corticosteroids and/or antibiotics). In this study, we hypothesize that a cat food with an omega-6 polyunsaturated fatty acid (ω6 PUFA) to ω3 PUFA ratio of 10:1 reduces inflammation of the FCGS and accelerates soft tissue wound healing of the gingiva after dental extractions, compared to a cat food with a ω6:ω3 PUFA ratio of 40:1. The cats were fed diets with chicken fat and fish oil as sources of fatty acids. In one diet, part of the fish oil was replaced by safflower oil, resulting in two diets with ω6:ω3 PUFA ratios of 10:1 and 40:1. This double-blinded study in two groups of seven cats revealed that dietary fatty acids influence the composition of plasma cholesteryl esters and plasma levels of inflammatory cytokines. The diet with the 10:1 ratio lowered PGD(2) , PGE(2) and LTB(4) plasma levels significantly, compared to the diet with the 40:1 ratio (p = 0.05, p = 0.04, and p = 0.02 respectively). However, feeding diets with dietary ω6:ω3 PUFA ratios of 10:1 and 40:1, given to cats with FCGS for 4 weeks after extraction of all premolars and molars, did not alter the degree of inflammation or wound healing.

  2. Aloe vera extract activity on human corneal cells.

    Science.gov (United States)

    Woźniak, Anna; Paduch, Roman

    2012-02-01

    Ocular diseases are currently an important problem in modern societies. Patients suffer from various ophthalmologic ailments namely, conjunctivitis, dry eye, dacryocystitis or degenerative diseases. Therefore, there is a need to introduce new treatment methods, including medicinal plants usage. Aloe vera [Aloe barbadensis Miller (Liliaceae)] possesses wound-healing properties and shows immunomodulatory, anti-inflammatory or antioxidant activities. NR uptake, MTT, DPPH• reduction, Griess reaction, ELISA and rhodamine-phalloidin staining were used to test toxicity, antiproliferative activity, reactive oxygen species (ROS) reduction, nitric oxide (NO) and cytokine level, and distribution of F-actin in cells, respectively. The present study analyzes the effect of Aloe vera extracts obtained with different solvents on in vitro culture of human 10.014 pRSV-T corneal cells. We found no toxicity of ethanol, ethyl acetate and heptane extracts of Aloe vera on human corneal cells. No ROS reducing activity by heptane extract and trace action by ethanol (only at high concentration 125 µg/ml) extract of Aloe vera was observed. Only ethyl acetate extract expressed distinct free radical scavenging effect. Plant extracts decreased NO production by human corneal cells as compared to untreated controls. The cytokine (IL-1β, IL-6, TNF-α and IL-10) production decreased after the addition of Aloe vera extracts to the culture media. Aloe vera contains multiple pharmacologically active substances which are capable of modulating cellular phenotypes and functions. Aloe vera ethanol and ethyl acetate extracts may be used in eye drops to treat inflammations and other ailments of external parts of the eye such as the cornea.

  3. Incidence of distal caries in mandibular second molars due to impacted third molars: Nonintervention strategy of asymptomatic third molars causes harm? A retrospective study.

    Science.gov (United States)

    Srivastava, Nikhil; Shetty, Akshay; Goswami, Rahul Dev; Apparaju, Vijay; Bagga, Vivek; Kale, Saurabh

    2017-01-01

    Removal of impacted third molars is the most common oral surgical procedure. Many investigators have questioned the necessity of removal in patients who are free of symptoms or associated pathologies. The aim of this retrospective study was to evaluate the incidence of caries on distal aspect of mandibular second molars in patients referred for corresponding third molar assessment and to identify its association with angular position and depth of the impacted mandibular third molars based on the classification of Pell and Gregory. Records of 150 patients with impacted third molar presenting to the Department of Oral and Maxillofacial Surgery, Sri Rajiv Gandhi College of Dental Sciences and Hospital, were assessed retrospectively. The radiographic angulation and depth of mandibular third molar impaction were determined and compared to determine the relationship with incidence of caries on the distal surface of the second molar. According to this study results, 37.5% cases show caries on the distal aspect of mandibular second molars. The incidence of caries with mesioangular impacted third molars was 55%. A majority of these mesioangular cases were Level B and Class I as per the Pell and Gregory classification. The prophylactic extraction of mandibular third molars is indicated if the angulation is between 30° and 70° and is justified by incidence of distal caries in the second molars.

  4. Incidence of distal caries in mandibular second molars due to impacted third molars: Nonintervention strategy of asymptomatic third molars causes harm? A retrospective study

    Science.gov (United States)

    Srivastava, Nikhil; Shetty, Akshay; Goswami, Rahul Dev; Apparaju, Vijay; Bagga, Vivek; Kale, Saurabh

    2017-01-01

    Background: Removal of impacted third molars is the most common oral surgical procedure. Many investigators have questioned the necessity of removal in patients who are free of symptoms or associated pathologies. Aim: The aim of this retrospective study was to evaluate the incidence of caries on distal aspect of mandibular second molars in patients referred for corresponding third molar assessment and to identify its association with angular position and depth of the impacted mandibular third molars based on the classification of Pell and Gregory. Methodology: Records of 150 patients with impacted third molar presenting to the Department of Oral and Maxillofacial Surgery, Sri Rajiv Gandhi College of Dental Sciences and Hospital, were assessed retrospectively. The radiographic angulation and depth of mandibular third molar impaction were determined and compared to determine the relationship with incidence of caries on the distal surface of the second molar. Results: According to this study results, 37.5% cases show caries on the distal aspect of mandibular second molars. The incidence of caries with mesioangular impacted third molars was 55%. A majority of these mesioangular cases were Level B and Class I as per the Pell and Gregory classification. Conclusion: The prophylactic extraction of mandibular third molars is indicated if the angulation is between 30° and 70° and is justified by incidence of distal caries in the second molars. PMID:28251102

  5. Effect of submucosal or oral administration of prednisolone on postoperative sequelae following surgical extraction of impacted mandibular third molar: A randomized controlled study

    Directory of Open Access Journals (Sweden)

    Adebayo Aremu Ibikunle

    2016-01-01

    Full Text Available Background: The aim of the study was to evaluate the effect of preoperatively administered submucosal and oral prednisolone on postoperative pain, facial swelling, and trismus following third molar surgery. Patients and Methods: This was a randomized controlled trial in which subjects were randomly distributed into three groups. Group A consisted of subjects who received 40 mg oral prednisolone; Group B consisted of subjects who received 40 mg submucosal injection of prednisolone while Group C consisted of subjects who did not receive prednisolone. Each group had 62 subjects. Measurements for facial width/facial swelling, pain, and mouth opening were recorded preoperatively and postoperatively. The postoperative evaluation points were postoperative days 1, 3, and 7. These measurements were compared with the preoperative values both within and among the groups. Results: Most of the subjects were in their third decade of life. A considerable increase in the mean postoperative values for pain, facial width and trismus was observed. Notably, subjects who did not receive prednisolone showed comparatively higher values for the measured parameters throughout the postoperative evaluation period. Subjects who received submucosal injection of prednisolone showed overall lower values compared to those who received oral prednisolone. Conclusion: The results of this study indicate that the administration of prednisolone has a significantly beneficial effect in ameliorating the postoperative sequelae of the third molar surgery. In addition, the effect of submucosally injected prednisolone is comparable to the orally administered prednisolone; indeed it shows superiority to the latter in a number of dimensions. Submucosal injection of prednisolone offers a simple, effective, easy, safe, and minimally invasive option to existing therapeutic methods of reducing these postoperative sequelae.

  6. Effect of submucosal or oral administration of prednisolone on postoperative sequelae following surgical extraction of impacted mandibular third molar: A randomized controlled study

    Science.gov (United States)

    Ibikunle, Adebayo Aremu; Adeyemo, Wasiu Lanre; Ladeinde, Akinola Ladipo

    2016-01-01

    Background: The aim of the study was to evaluate the effect of preoperatively administered submucosal and oral prednisolone on postoperative pain, facial swelling, and trismus following third molar surgery. Patients and Methods: This was a randomized controlled trial in which subjects were randomly distributed into three groups. Group A consisted of subjects who received 40 mg oral prednisolone; Group B consisted of subjects who received 40 mg submucosal injection of prednisolone while Group C consisted of subjects who did not receive prednisolone. Each group had 62 subjects. Measurements for facial width/facial swelling, pain, and mouth opening were recorded preoperatively and postoperatively. The postoperative evaluation points were postoperative days 1, 3, and 7. These measurements were compared with the preoperative values both within and among the groups. Results: Most of the subjects were in their third decade of life. A considerable increase in the mean postoperative values for pain, facial width and trismus was observed. Notably, subjects who did not receive prednisolone showed comparatively higher values for the measured parameters throughout the postoperative evaluation period. Subjects who received submucosal injection of prednisolone showed overall lower values compared to those who received oral prednisolone. Conclusion: The results of this study indicate that the administration of prednisolone has a significantly beneficial effect in ameliorating the postoperative sequelae of the third molar surgery. In addition, the effect of submucosally injected prednisolone is comparable to the orally administered prednisolone; indeed it shows superiority to the latter in a number of dimensions. Submucosal injection of prednisolone offers a simple, effective, easy, safe, and minimally invasive option to existing therapeutic methods of reducing these postoperative sequelae. PMID:27833246

  7. 反角高速涡轮机拔除低位下颌阻生齿的效果观察%Contra-angle High Speed Turbine Handpiece in the Extraction of Impacted Mandibular Third Molars

    Institute of Scientific and Technical Information of China (English)

    刘荫; 刘唯; 李红玖; 杨安; 程由勇

    2015-01-01

    Objective: To discuss the clinical efficacy of contra-angle high speed turbine handpiece in the extraction of impacted mandibular third molar. Methods: Extraction of 194 impacted mandibular wisdom teeth were randomly divided into two groups:in experimental group (107 cases), mandibular impacted wisdom teeth were extracted by contra-angle high speed turbine handpiece, and in control group (87 cases), traditional chisels were used. The postoperative situations between two groups were compared. Results:The time of extraction in two groups were similar(P>0.05). The postoperative swelling, limited mouth opening, postoperative pain levels of experimental group were lower than in the control group, the difference was statistically significant (P0.05),涡轮机组术后肿胀程度、张口受限情况、术后疼痛程度均小于对照组,差异有统计学意义(P<0.05)。结论:使用反角高速手机拔除下颌低位阻生智齿,操作简便,相对于传统劈冠法,术后反应较轻,减轻了患者的痛苦,值得推广。

  8. Rapid Extraction of Human DNA Containing Humic Acid

    OpenAIRE

    Sutlović, Davorka; Definis Gojanović, Marija; Anđelinović, Šimun

    2007-01-01

    The identification process of dead bodies or human remains is nowadays conducted in numerous fields of forensic science, archeology and other judicial cases. A particular problem is the isolation and DNA typing of human remains found in mass graves, due to the degradation process, as well as post mortal DNA contamination with bacteria, fungi, humic acids, metals, etc. In this study, the influence of humic acid (HA) on the DNA extraction and typing is investigated. If present in...

  9. Ruptured tubal molar pregnancy

    African Journals Online (AJOL)

    2011-05-19

    May 19, 2011 ... disorders (invasive mole, placental site trophoblastic tumor ... Molar pregnancies in most instances develop within the uterine cavity, but may occur at any site. ... closed cervix and positive cervical excitation tenderness.

  10. Autotransplantation of Mandibular Third Molar: A Case Report

    Directory of Open Access Journals (Sweden)

    Pabbati Ravi kumar

    2012-01-01

    Full Text Available Autogenous transplantation is a feasible, fast, and economical option for the treatment of nonsalvageable teeth when a suitable donor tooth is available. This paper presents successful autotransplantation of a mature mandibular left third molar (38 without anatomical variances is used to replace a mandibular left second molar (37. The mandibular second molar was nonrestorable due to extensive root caries and resorption of distal root. After extraction of mandibular second and third molars, root canal therapy was done for the third molar extraorally, and the tooth was reimplanted into the extracted socket of second molar site. After one year, clinical and radiographic examination revealed satisfactory outcome with no signs or symptoms suggestive of pathology. In selected cases, autogenous tooth transplantation, even after complete root formation of the donor tooth, may be considered as a practical treatment alternative to conventional prosthetic rehabilitation or implant treatment.

  11. A randomized, controlled study to investigate the analgesic efficacy of single doses of the cannabinoid receptor-2 agonist GW842166, ibuprofen or placebo in patients with acute pain following third molar tooth extraction.

    Science.gov (United States)

    Ostenfeld, Thor; Price, Jeffrey; Albanese, Massimo; Bullman, Jonathan; Guillard, Fiona; Meyer, Ingo; Leeson, Rachel; Costantin, Cristina; Ziviani, Luigi; Nocini, Pier Francesco; Milleri, Stefano

    2011-10-01

    To evaluate the postoperative analgesic efficacy of GW842166, a noncannabinoid CB2 agonist, in patients undergoing third molar tooth extraction. This randomized, double-blind, placebo-controlled study compared the analgesic efficacy of single doses of GW842166 (100 or 800 mg) or ibuprofen with placebo in patients undergoing extraction of at least 1 fully or partially impacted third molar tooth. Eligible participants were dosed preoperatively within 1 hour of surgery. Participants allocated to active comparator received a second dose of ibuprofen (400 mg), 4 hours after the first 800 mg dose. Participants in the GW842166 and placebo groups received placebo at 4 hours. Procedures for the assessment of efficacy included a visual analog scale and verbal rating scale for scoring pain up to 10 hours postsurgery, duration of analgesia, patient global evaluation, proportion of patients requiring rescue medication, and elapsed time to rescue analgesia. Analysis of covariance was used to compare efficacy variables. Patient global evaluation was analyzed using Wilcoxon rank-sum tests and time to data was analyzed using the log-rank test. Ibuprofen was significantly more effective than placebo across all endpoints. Trends for an improvement in pain scores for GW842166 800 mg failed to be of either clinical or statistical significance. GW842166 100 mg showed little separation from placebo. There was no evidence for any beneficial adjunctive effect after coadministration of rescue analgesia with GW842166. All treatments were well tolerated. In comparison to ibuprofen, single doses of GW842166 (100 and 800 mg) failed to demonstrate clinically meaningful analgesia in the setting of acute dental pain.

  12. Fracture Resistance of Pulpotomized Primary Molar Restored with Extensive Class II Amalgam Restorations

    Directory of Open Access Journals (Sweden)

    F. Mazhari

    2008-06-01

    Full Text Available Objective: The aim of the present study was to evaluate fracture resistance of pulpoto-mized primary molar teeth restored with extensive multisurface amalgam restorations.Materials and Methods: Eighty extracted carious human primary molar teeth were se-lected forpresent study. Teeth were divided in to eight groups of ten. Mesio- or disto-occlusal and Mesio-occluso-distal cavities with different cavity wall thickness (1.5 or 2.5mm were prepared in both first and second primary molar teeth. After restoring teeth with amalgam, all specimens were stored in distilled water at 37°C for 7 days. Then samples were thermocycled for 1000 cycles from 5°C to 55°C. The specimens then were subjected to a compressive load in a universal testing machine at a crosshead speed of 0.5 mm min-1. ANOVA and t-test were used for statistical analysis.Results: Mean fracture resistance of first and second molar teeth were 975.5 N (SD=368.8 and 1049.2 N (SD=540.1 respectively. In the first molar group, fracture resis-tance of two-surface cavities was significantly more than three-surface cavities (P<0.001, however this difference was not statistically significant in the second molar group. In both first and second molar group, fracture resistance incavities with 2.5 mm wall thickness, was significantly more than the group with 1.5 mm wall thickness.Conclusion: The mean fracture resistance in pulpotomized primary molar restored with amalgam restorations was higher than reported maximum bite force in primary teeth even in extensive multi-surface restorations. Therefore, the teeth with large proximal carious lesions in schoolchildren could be restored with amalgam.

  13. [New surgical approach in apicoectomy of maxillary molars' palatal root].

    Science.gov (United States)

    Hrusztics, Aminett; Bogdán, Sándor; Fellegi, Veronika; Szabó, György

    2003-06-01

    The term of apicectomy has been well-known for more than 200 years, nevertheless it is not performed frequently on molars. As a result of this a lot of molars became extracted. The aim of the authors was to present the new surgical technique which is described in detail. The radicular cyst localised on the palatal root of the first maxillary molar was operated on. Uneventful healing was obtained. This surgical technique is recommended in some special cases.

  14. Sterilisation of extracted human teeth for educational use

    Directory of Open Access Journals (Sweden)

    Kumar M

    2005-01-01

    Full Text Available Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10% formalin for seven days, group 2 teeth were immersed in 3% hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6% sodium hypochlorite for seven days, group 4 teeth were boiled in water at 100°C for 20 minutes, group 5 teeth were autoclaved at 121°C at 15 lbs psi for 30 minutes, and group 6 teeth were immersed in normal saline for seven days. After the treatment, the teeth were individually inoculated into trypticase soy broth and incubated for 48 hours. A questionnaire survey was also conducted to determine the awareness of dental students regarding infection due to extracted human teeth and the common disinfection/sterilisation methods used. Autoclaving at 121°C, 15 lbs psi for 30 minutes and immersion in 10% formalin for seven days were effective in disinfecting/sterilising extracted human teeth. Chemicals such as 2.6% sodium hypochlorite, 3% hydrogen peroxide and boiling in water were not effective. The results indicate that autoclaving for 30 minutes or immersion in 10% formalin for seven days could be effectively used for disinfection/sterilisation of extracted human teeth.

  15. Analysis of human muscle extracts by proton NMR

    Energy Technology Data Exchange (ETDEWEB)

    Venkatasubramanian, P.N.; Barany, M.; Arus, C.

    1986-03-01

    Perchloric acid extracts were prepared from pooled human muscle biopsies from patients diagnosed with scoliosis (SCOL) and cerebral palsy (CP). After neutralization with KOH and removal of perchlorate, the extracts were concentrated by freeze drying and dissolved in /sup 2/H/sub 2/O to contain 120 O.D. units at 280 nm per 0.5 ml. /sup 1/H NMR spectroscopy was performed with the 5 mm probe of a Varian XL300 instrument. Creatine, lactate, carnosine, and choline were the major resonances in the one-dimensional spectra of both extracts. With creatine as reference, 2.5-fold more lactate was found in SCOL than in CP, and a much smaller difference was also found in their carnosine content. Two-dimensional COSY comparison revealed several differences between the two extracts. Taurine, N-acetyl glutamate, glycerophosphoryl choline (or phosphoryl choline) and an unidentified spot were present only in the extract from SCOL but not in that from CP. On the other hand, aspartate, hydroxy-proline, carnitine and glycerophosphoryl ethanolamine were only present in CP but absent in SCOL. Alanine, cysteine, lysine and arginine appeared in both extracts without an apparent intensity difference.

  16. Comparison of different extractions of impacted mandible third molars%两种方法在拔除下颌阻生第三磨牙中的疗效比较

    Institute of Scientific and Technical Information of China (English)

    刘鹏; 赵枫; 袁伟; 徐建立; 李东临; 付崇建; 朱国雄

    2013-01-01

    目的:观察和比较两种拔除下颌阻生第三磨牙的临床疗效.方法:门诊200例下颌阻生第三磨牙随机分为两组,各100颗.分别采用传统锤凿去骨法和涡轮机去骨法拔除,记录手术时间、术后疼痛、张口受限等情况并进行统计学分析.结果:两种手术方法在手术时间、术后疼痛、张口受限、肿胀程度等方面有统计学差异(P<0.05),传统锤凿去骨组出现干槽症与下唇麻木分别为5例和1例,涡轮机去骨组分别为1例和0例,组间比较差异无统计学意义(P>0.05).结论:与传统锤凿去骨法相比,采用涡轮机去骨法拔除下颌阻生第三磨牙手术时间短,术后并发症较少,值得临床推广.%Objective To observe and compare the outcomes of impacted mandibular third molar extraction by 2 kinds of methods. Methods 200 impacted mandibular third molars were randomly divided into two groups: 100 teeth in traditional hammer and chisel extraction group and 100 teeth in high-speed turbine extraction group.The operating time, postoperative complications were observed. Results There was significantly difference between two groups in the operating time,the ache of postoperative, a reduced mouth opening and regional swelling(P<0.05).While there was no significant difference in the incidence of dry socket and numbness of lower lip. Conclusion Compared with trational hammer and chisel technique, high-speed turbine extraction could shorten operating time and reduce the postoperaive complications.

  17. Second mesiobuccal canal detection in maxillary first molars using microscopy and ultrasonics.

    Science.gov (United States)

    Alaçam, Tayfun; Tinaz, Ali Cemal; Genç, Ozgür; Kayaoglu, Guven

    2008-12-01

    The aim of this study was to investigate whether the use of operating microscope in combination with ultrasonics increased the rate of second mesiobuccal (MB2) canal detection in permanent maxillary first molar teeth. A hundred extracted human maxillary first molars were assessed. After location of the main canals, the MB2 canal was sought in all teeth first without microscopy, then with the aid of the operating microscope and finally with the combined use of the operating microscope and ultrasonics. With these techniques, the MB2 canal was detected in 62%, 67% and 74% of the teeth, respectively. The combination of the operating microscope and ultrasonics detected significantly more MB2 canals than when no microscopy was utilized (P ultrasonics increased the detection of MB2 canals in maxillary first permanent molars.

  18. The Application of CBCT in the Extraction of the Third Impacted Mandible Molar%CBCT 在下颌第三磨牙阻生拔除术中的应用

    Institute of Scientific and Technical Information of China (English)

    赵国廷; 李伟; 姚毅章

    2014-01-01

    Objectives :This paper compares and analyzes the extraction of third impacted molar under the CBCT and mediated turbine drill in our department and relevant complications by the way of conven -tional X - ray .What’s more ,it also analyzes the likeliest causes and put forward some preventive meth-ods ,meanwhile it helps us to understand the subjective satisfaction of patients after operation .Methods :This paper has analyzed 54 cases of the extraction of the third impacted mandible molars under the CBCT , conventional X - ray ,panoramic radiographs and mediated turbine drill . There are 12 cases of surgery complications during and after operation .At last ,it evaluates the subjective satisfaction of patients before and after the operation .Results :These 26 extraction cases with CBCT mediated by turbine drill ,among which there are 3 cases with surgery complications during and after the operation .It accounts for 11 .5% ;while other 28 extraction cases under the conventional X - ray or panoramic radiographs of turbine drill , there are 9 cases of surgery complications before or after the operation ,which accounts for 32% .The pa-tients’ percentage of subjective satisfaction with CBCT mediated operation was much higher than that of conventional ones .Conclusion :The applications of CBCT mediated turbine drill in extraction of the impact -ed third mandible molar can decrease the relevant surgery complications .In this way ,it can make the pa-tients feel satisfactory .%目的:对比分析我科在 CBCT 介导下涡轮机钻法与常规 X 线介导下涡轮机钻法下颌第三磨牙阻生拔除术中及术后出现并发症的情况,分析其可能原因及预防方法,并了解患者对手术主观满意度.方法:分析在 CBCT 介导下和常规 X 线片或曲面断层片介导涡轮机钻法拔除下颌第三磨牙阻生54例,出现术中或术后并发症的12例.术后患者对手术前、后主观满意度评价.结果:采用 CBCT 介

  19. New quantitative classification of the anatomical relationship between impacted third molars and the inferior alveolar nerve

    OpenAIRE

    Wang, Wei-Quan; Chen, Michael Y.C.; Huang, Heng-Li; Fuh, Lih-Jyh; Tsai, Ming-Tzu; Hsu, Jui-Ting

    2015-01-01

    Background Before extracting impacted lower third molars, dentists must first identify the spatial relationship between the inferior alveolar nerve (IAN) and an impacted lower third molar to prevent nerve injury from the extraction. Nevertheless, the current method for describing the spatial relationship between the IAN and an impacted lower third molar is deficient. Therefore, the objectives of this study were to: (1) evaluate the relative position between impacted lower third molars and the...

  20. Rapid extraction and preservation of genomic DNA from human samples.

    Science.gov (United States)

    Kalyanasundaram, D; Kim, J-H; Yeo, W-H; Oh, K; Lee, K-H; Kim, M-H; Ryew, S-M; Ahn, S-G; Gao, D; Cangelosi, G A; Chung, J-H

    2013-02-01

    Simple and rapid extraction of human genomic DNA remains a bottleneck for genome analysis and disease diagnosis. Current methods using microfilters require cumbersome, multiple handling steps in part because salt conditions must be controlled for attraction and elution of DNA in porous silica. We report a novel extraction method of human genomic DNA from buccal swab and saliva samples. DNA is attracted onto a gold-coated microchip by an electric field and capillary action while the captured DNA is eluted by thermal heating at 70 °C. A prototype device was designed to handle four microchips, and a compatible protocol was developed. The extracted DNA using microchips was characterized by qPCR for different sample volumes, using different lengths of PCR amplicon, and nuclear and mitochondrial genes. In comparison with a commercial kit, an equivalent yield of DNA extraction was achieved with fewer steps. Room-temperature preservation for 1 month was demonstrated for captured DNA, facilitating straightforward collection, delivery, and handling of genomic DNA in an environment-friendly protocol.

  1. Water extraction on Mars for an expanding human colony.

    Science.gov (United States)

    Ralphs, M; Franz, B; Baker, T; Howe, S

    2015-11-01

    In-situ water extraction is necessary for an extended human presence on Mars. This study looks at the water requirements of an expanding human colony on Mars and the general systems needed to supply that water from the martian atmosphere and regolith. The proposed combination of systems in order to supply the necessary water includes a system similar to Honeybee Robotics' Mobile In-Situ Water Extractor (MISWE) that uses convection, a system similar to MISWE but that directs microwave energy down a borehole, a greenhouse or hothouse type system, and a system similar to the Mars Atmospheric Resource Recovery System (MARRS). It is demonstrated that a large water extraction system that can take advantage of large deposits of water ice at site specific locations is necessary to keep up with the demands of a growing colony.

  2. Curative effect comparison between two different methods of extracting mandibular third molars%两种方法拔除下颌第三磨牙的疗效比较

    Institute of Scientific and Technical Information of China (English)

    李传祝

    2014-01-01

    Objective To compare the clinical effects of traditional chisel method and high-speed air turbine drill method of extrac-ting impacted mandibular wisdom teeth,and to evaluate the clinical feasibility and superiority of the high-speed air turbine drill method. Methods 500 patients with impacted mandibular third molars were randomly divided into 2 grouptest group and control group.260 cases (test group)used the high-speed air turbine drill and new instruments to extract the impacted mandibular third molars while other 240 cases (control group)used the bone chiseling and dental crown hacking method to extract the impacted mandibular third molars. The operation time and post-operative complications were observed and recorded to evaluate the effects of the two methods.Results The data showed that the operation time of the test group was obviously shorter than the control group (P<0.05).There was signifi-cant difference in the degree of pain and limitation of mouth opening degree(P<0.05)after the operation between two groups.The in-cidence of alveolalgia of the test group was apparently lower than that of the control group.Conclusions The high-speed air turbine drill and new instruments method of extracting impacted mandibular wisdom teeth can efficiently shorten the operation time,reduce the incidence of complications,so it is worthy to be promoted and widely used.%目的:对比传统的凿骨劈冠法和改良涡轮机拔除法拔除下颌阻生第三磨牙时的临床效果,评价改良涡轮机拔除法的可行性和优越性。方法选择500例下颌阻生第三磨牙病例,其中260例(实验组)使用改良涡轮机拔除,另240例(对照组)用传统凿骨劈冠法拔除,对手术时间、术后并发症进行分析观察和统计分析。结果实验组手术时间明显比对照组短(P<0.05),两组患者术后疼痛程度和张口受限程度的构成比存在明显性差异(P<0.05),两组干槽症的发

  3. Comparison of piezosurgery and chisel osteotomy in the extraction of mandibular impacted third molars%超声骨刀与凿骨劈冠法拔除下颌阻生智齿的比较

    Institute of Scientific and Technical Information of China (English)

    高永波; 蒋瑷; 李伯友; 杨立明

    2011-01-01

    目的 探讨超声骨刀拔除下颌阻生智齿的效果.方法 根据下颌阻生第三磨牙拔除难度判断方案选择拔牙难度判断为较难的228颗下颌阻生智齿,分成实验组和对照组,每组114颗,实验组应用超声骨刀法拔牙,对照组采用凿骨劈冠法拔牙.对2组的拔牙时间、术后疼痛、张口受限、面部肿胀情况进行分析比较.结果 实验组平均拔牙时间为(16+5.2)min,对照组平均拔牙时间为(30±8.7)min.实验组的拔牙时间、术后疼痛、面部肿胀率及张口受限均低于对照组,两组间的差异均具有统计学意义(P<0.05).结论 超声骨刀法拔除下颌阻生智齿较凿骨劈冠法手术时间短,术后并发症轻.%Objective To study the effect of piezosurgery in the extraction of mandibular impacted third molars. Methods 228 mandibular impacted third molars with relative difficulty for extraction according to the scheme of de gree of difficulty for extraction were included in the study, and were divided into two groups (114 teeth each group): Test group (treated by piezosurgery), control group (treated by traditional chisel osteotomy). The surgery time and pain, restriction of mouth opening and facial swelling after surgery, were evaluated in both groups. Results The average sur gery time was (16±5.2) min in test group and (30±8.7) min in control group. The surgery time, pain, facial swelling rate, and restriction of mouth opening were lower than control group (P<0.05). Conclusion Compared with traditional chisel osteotomy, the average surgery time with piezosurgery was significantly shorter and the complications are obviously reduced.

  4. Placebo-controlled randomized clinical trial of the effect two different low-level laser therapies (LLLT)--intraoral and extraoral--on trismus and facial swelling following surgical extraction of the lower third molar.

    Science.gov (United States)

    Aras, Mutan Hamdi; Güngörmüş, Metin

    2010-09-01

    in the placebo group (29.0 +/- 6.2 mm). This study demonstrates that extraoral LLLT is more effective than intraoral LLLT for the reduction of postoperative trismus and swelling after extraction of the lower third molar.

  5. Bilateral Transverse Mandibular Second Molars: A Case Report

    Directory of Open Access Journals (Sweden)

    James Puryer

    2016-11-01

    Full Text Available Impaction of mandibular second permanent molars is a rare occurrence, with prevalence rates reported to be between 0.65% and 2.0%. In the absence of systemic conditions, impactions are usually unilateral. There appears to be no consensus as to the optimal treatment for impacted mandibular second molars and treatment plans will be based upon the individual case. Treatment may involve orthodontics and/or various surgical techniques, and early diagnosis is important. This paper presents an unusual case of bilateral transverse impaction of both mandibular second and third molars that was diagnosed at 18 years of age. All impacted molars were extracted.

  6. Trinucleotide repeat expansions catalyzed by human cell-free extracts

    Institute of Scientific and Technical Information of China (English)

    Jennifer R Stevens; Elaine E Lahue; Guo-Min Li; Robert S Lahue

    2013-01-01

    Trinucleotide repeat expansions cause 17 heritable human neurological disorders.In some diseases,somatic expansions occur in non-proliferating tissues such as brain where DNA replication is limited.This finding stimulated significant interest in replication-independent expansion mechanisms.Aberrant DNA repair is a likely source,based in part on mouse studies showing that somatic expansions are provoked by the DNA repair protein MutSβ (Msh2-Msh3complex).Biochemical studies to date used cell-free extracts or purified DNA repair proteins to yield partial reactions at triplet repeats.The findings included expansions on one strand but not the other,or processing of DNA hairpin structures thought to be important intermediates in the expansion process.However,it has been difficult to recapitulate complete expansions in vitro,and the biochemical role of MutSβ remains controversial.Here,we use a novel in vitro assay to show that human cell-free extracts catalyze expansions and contractions of trinucleotide repeats without the requirement for DNA replication.The extract promotes a size range of expansions that is similar to certain diseases,and triplet repeat length and sequence govern expansions in vitro as in vivo.MutSβ stimulates expansions in the extract,consistent with aberrant repair of endogenous DNA damage as a source of expansions.Overall,this biochemical system retains the key characteristics of somatic expansions in humans and mice,suggesting that this important mutagenic process can be restored in the test tube.

  7. Clinical significance of computed tomography assessment for third molar surgery

    Institute of Scientific and Technical Information of China (English)

    Kenji; Nakamori; Kei; Tomihara; Makoto; Noguchi

    2014-01-01

    Surgical extraction of the third molar is the most commonly performed surgical procedure in the clinical practice of oral surgery.Third molar surgery is warranted when there is inadequate space for eruption,malpositioning,or risk for cyst or odontogenic tumor formation.Preoperative assessment should include a detailed morphologic analysis of the third molar and its relationship to adjacent structures and surrounding tissues.Due to developments in medical engineering technology,computed tomography(CT)now plays a critical role in providing the clear images required for adequate assessment prior to third molar surgery.Removal of the maxillary third molar is associated with a risk for maxillary sinus perforation,whereas removal of the mandibular third molar can put patients at risk for a neurosensory deficit from damage to the lingual nerve or inferior alveolar nerve.Multiple factors,including demographic,anatomic,and treatment-related factors,influence the incidence of nerve injury during or following removal of the third molar.CT assessment of the third molar prior to surgery can identify some of these risk factors,such as the absence of cortication between themandibular third molar and the inferior alveolar canal,prior to surgery to reduce the risk for nerve damage.This topic highlight presents an overview of the clinical significance of CT assessment in third molar surgery.

  8. Clinical significance of computed tomography assessment for third molar surgery.

    Science.gov (United States)

    Nakamori, Kenji; Tomihara, Kei; Noguchi, Makoto

    2014-07-28

    Surgical extraction of the third molar is the most commonly performed surgical procedure in the clinical practice of oral surgery. Third molar surgery is warranted when there is inadequate space for eruption, malpositioning, or risk for cyst or odontogenic tumor formation. Preoperative assessment should include a detailed morphologic analysis of the third molar and its relationship to adjacent structures and surrounding tissues. Due to developments in medical engineering technology, computed tomography (CT) now plays a critical role in providing the clear images required for adequate assessment prior to third molar surgery. Removal of the maxillary third molar is associated with a risk for maxillary sinus perforation, whereas removal of the mandibular third molar can put patients at risk for a neurosensory deficit from damage to the lingual nerve or inferior alveolar nerve. Multiple factors, including demographic, anatomic, and treatment-related factors, influence the incidence of nerve injury during or following removal of the third molar. CT assessment of the third molar prior to surgery can identify some of these risk factors, such as the absence of cortication between the mandibular third molar and the inferior alveolar canal, prior to surgery to reduce the risk for nerve damage. This topic highlight presents an overview of the clinical significance of CT assessment in third molar surgery.

  9. Prevention of Alveolar Osteitis After Third Molar Surgery ...

    African Journals Online (AJOL)

    2017-05-16

    May 16, 2017 ... of alveolar osteitis after third molar surgery: Comparative study of the effect of warm saline ... recovery after dental extractions as evidenced by reports across the ... routine use after oral surgical procedures not to be common.

  10. Evaluation of the analgesic effect of a partial closure after the impacted mandibular third molar extraction%下颌阻生牙拔除后两种镇痛方法效果的观察

    Institute of Scientific and Technical Information of China (English)

    高巍; 姜霞

    2011-01-01

    目的 探讨下颌阻生牙拔除后两种镇痛方法的效果.方法 将62例患者分为两组,试验组(32例)拔牙后用甲哌卡因局部封闭,对照组(30例)术后2h服用布洛芬缓释胶囊0.3g,此后每隔6h口服一次,至术后48 h结束.比较两种方法术后镇痛效果.结果 试验组术后2h平均疼痛评分(2.5±1.1)低于对照组(2.8±1.1),但两组患者术后2~48 h疼痛程度差异无统计学意义(P>0.05),疼痛评分与年龄、性别、分组均无关,仅与术后时间有关(P<0.05).结论 下颌阻生牙拔除术后局部封闭可取得与服用布洛芬缓释胶囊相同的镇痛效果.%Objective The study is aimed to compare the analgesic effect of two preemptive analgesia programs (partial closure / fenbid po.) in patients undergoing extraction of the impacted mandibular third molar.Methods Sixty-two patients who visited our outpatient center from December 2009 to September 2010 with impacted mandibular third molar were randomly divided into two groups.Patients after extraction were given 3% mepivacaine injecting immediately under the periosteum for partial closure in the experimental group,while them in the control group,fenbid 300 mg were administrated at 2 hours after operation and once more every 6 hours.Pain sores at 2 h,4 h,8 h,12 h,24 h,48 h after operation were recorded with VAS (visual analogue scale) and parallel groups repeated measures design of longitudinal studies was carried out for statistical comparison between the two groups:studies in which individuals were measured repeatedly through after surgical operation time (2 h,4 h,… ).Results At the time of 2 hours after the operation,the average pain scores of the mepivacaine group (2.5 ± 1.1 ) was lower than the pain scores of the fenbid group (2.8 ± 1.1 ).But there was no significant difference in pain scores between two groups within 2 - 48 h after operation ( P > 0.05 ).Also no statistically relationship between the pain scores and the factors

  11. 应用PAR指数评价拔除龋坏第一恒磨牙的矫治效果%Evaluation of orthodontic treatment with carious first molars extraction by PAR index

    Institute of Scientific and Technical Information of China (English)

    田玉楼; 王鑫; 王坤; 刘芳

    2012-01-01

    目的 应用PAR指数评价拔除龋坏第一恒磨牙正畸治疗的临床效果.方法 对20例拔除1~4颗龋坏第一恒磨牙进行矫治的病例做回顾性分析研究.按照PAR指数的规则对20例患者治疗前后的石膏模型进行测量、评估、分析.结果 20例病例PAR总分值矫治前、后为29.50±7.05、2.00±2.00,减少27.50±6.39,差异有极显著性(P<0.01);加权PAR总分值矫治前、后为24.00±9.25、1.94±2.54,减少22.06±9.75,差异有显著性意义(P<0.01);PAR指数各项指标中包括牙齿排列、颊侧(牙合)关系、覆盖、覆(牙合)等矫治前后分值差异均有极显著性(P<0.01),中线矫治前后分值差异有显著性(P<0.05).PAR总分值减少百分率为(93.63±6.02)%;加权PAR总分值减少百分率为(87.90±24.69)%.结论 应用PAR指数评价认为拔除龋坏第一恒磨牙进行正畸治疗能够取得理想的临床效果.%Objective To assess the clinical effects of orthodontic treatment with carious firat molars extraction by PAR ( Peer Assessment Rating) index. The aim of this research was to provide reference for clinical plan of orthodontic treatment. Methods 20 cases treated with carious first molars extraction were chosen. There were 6 males and 14 females,aged from 12 to 25 years with an average age of 14.5 years. All of them received orthodontic treatment with 1 lo 4 carious first molars extracted. Pretrealment and posttreat-mettf study models of 20 cases were assessed by PAR index. Results The PAR score of 20 cases was 29.50 ±7.05 before treatment, and was 2.00 ± 2.00 after the treatment, which decreased significantly after orthodontic treatment (P < 0. 01). The weighted PAR score of 20 cases was 24.00 ±9.25 before treatment,and was 1.94 ±2.54 after treatment,which decreased significantly (P<0.01). The PAR scores of the teeth alignment,buccal occlusion,overjet.overbite were significantly different after orthodontic treatment (P<0. 01). Centerline

  12. A Clinical Study of Extracting Third Molar Tooth Germ with the use of Piezosurgery and Turbine Dental Drill%超声骨刀高速牙钻拔除第3磨牙牙胚

    Institute of Scientific and Technical Information of China (English)

    郑杰; 王雷; 黄天鉴; 刘力佳; 杜玉芳; 陈红

    2012-01-01

      To explore the apply of the use of Piezosurgery and turbine dental drill in extracting third molar tooth germ. Methods: The data of 160 tooth extractions were analyzed, which include experimental group and control group.Results:In experimental group, operation complications were 1 example, after operation complications were 9 example. Adopt another method, operation complications were 11 example, after operation complications were 22 example.Two sets of method were significantly different (P<0.01) . the operating time of two method were no different. Conclusions:To pull out third molar tooth germ use the piezosurgery and turbine dental drill, the wound is small, apply accurate, be easy to a protection, can lower complications.%  目的:探讨超声骨刀与高速涡轮牙钻在第3磨牙牙胚拔除术中的应用效果.方法:选取需拔除的第3磨牙牙胚160例,随机分为超声骨刀+高速涡轮牙钻的实验组与高速涡轮牙钻对照组.对其术中、术后并发症产生的原因进行分析.结果:实验组术中并发症1例,术后并发症9例.对照组术中并发症11例,术后并发症22例,2组方法差异显著(P<0.01).手术完成时间实验组为38.71±19.58分钟,对照组为37.56±18.69分钟,2组无显著差异.结论:超声骨刀+高速涡轮牙钻拔除下颌阻生智齿,手术创伤小、神经及软组织保护性好、施力准确、易于控制,可大大降低术中、术后并发症.

  13. Iatrogenic Mandibular Fracture Associated with Third Molar Removal

    Directory of Open Access Journals (Sweden)

    Abdulkadir Burak Cankaya, Mehmet Ali Erdem, Sirmahan Cakarer, Muhsin Cifter, Cuneyt Korhan Oral

    2011-01-01

    Full Text Available Third molar extraction is one of the most common procedures performed in oral and maxillofacial surgery units. It is sometimes accompanied by complications such as alveolar osteitis, secondary infection, hemorrhage, dysesthesia and, most severely, iatrogenic fracture. This article describes two mandibular angle fractures that occurred in two patients during the surgical extraction of one erupted and one unerupted third molar, including a brief review of the literature.

  14. Iatrogenic Mandibular Fracture Associated with Third Molar Removal

    OpenAIRE

    Abdulkadir Burak Cankaya, Mehmet Ali Erdem, Sabri Cemil Isler, Sabit Demircan, Merva Soluk, Cetin Kasapoglu, Cuneyt Korhan Oral

    2011-01-01

    Third molar extraction is one of the most common procedures performed in oral and maxillofacial surgery units. It is sometimes accompanied by complications such as alveolar osteitis, secondary infection, hemorrhage, dysesthesia and, most severely, iatrogenic fracture. This article describes two mandibular angle fractures that occurred in two patients during the surgical extraction of one erupted and one unerupted third molar, including a brief review of the literature.

  15. Hypomineralized Second Primary Molars as Predictor of Molar Incisor Hypomineralization

    OpenAIRE

    Negre-Barber, A.; Montiel-Company, J. M.; Boronat-Catalá, M.; M. Catalá-Pizarro; Almerich-Silla, J. M.

    2016-01-01

    Molar incisor hypomineralization (MIH) is a developmental defect of dental enamel that shares features with hypomineralized second primary molars (HSPM). Prior to permanent tooth eruption, second primary molars could have predictive value for permanent molar and incisor hypomineralization. To assess this possible relationship, a cross-sectional study was conducted in a sample of 414 children aged 8 and 9 years from the INMA cohort in Valencia (Spain). A calibrated examiner (linear-weighted Ka...

  16. Investigation of dental pulp stem cells isolated from discarded human teeth extracted due to aggressive periodontitis.

    Science.gov (United States)

    Sun, Hai-Hua; Chen, Bo; Zhu, Qing-Lin; Kong, Hui; Li, Qi-Hong; Gao, Li-Na; Xiao, Min; Chen, Fa-Ming; Yu, Qing

    2014-11-01

    Recently, human dental pulp stem cells (DPSCs) isolated from inflamed dental pulp tissue have been demonstrated to retain some of their pluripotency and regenerative potential. However, the effects of periodontal inflammation due to periodontitis and its progression on the properties of DPSCs within periodontally compromised teeth remain unknown. In this study, DPSCs were isolated from discarded human teeth that were extracted due to aggressive periodontitis (AgP) and divided into three experimental groups (Groups A, B and C) based on the degree of inflammation-induced bone resorption approaching the apex of the tooth root before tooth extraction. DPSCs derived from impacted or non-functional third molars of matched patients were used as a control. Mesenchymal stem cell (MSC)-like characteristics, including colony-forming ability, proliferation, cell cycle, cell surface antigens, multi-lineage differentiation capability and in vivo tissue regeneration potential, were all evaluated in a patient-matched comparison. It was found that STRO-1- and CD146-positive DPSCs can be isolated from human teeth, even in very severe cases of AgP. Periodontal inflammation and its progression had an obvious impact on the characteristics of DPSCs isolated from periodontally affected teeth. Although all the isolated DPSCs in Groups A, B and C showed decreased colony-forming ability and proliferation rate (P biomaterials were transplanted directly into an ectopic transplantation model. However, when cell-seeded scaffolds were placed in the root fragments of human teeth, all the cells formed significant dentin- and pulp-like tissues. The ability of DPSCs to generate dental tissues decreased when the cells were isolated from periodontally compromised teeth (P < 0.05). Again, increased periodontal destruction was not necessarily followed by a decrease in the amount of dentin- and pulp-like tissue formed. These findings provide preliminary evidence that periodontally compromised teeth might

  17. Chestnut extract induces apoptosis in AGS human gastric cancer cells.

    Science.gov (United States)

    Lee, Hyun Sook; Kim, Eun Ji; Kim, Sun Hyo

    2011-06-01

    In Korea, chestnut production is increasing each year, but consumption is far below production. We investigated the effect of chestnut extracts on antioxidant activity and anticancer effects. Ethanol extracts of raw chestnut (RCE) or chestnut powder (CPE) had dose-dependent superoxide scavenging activity. Viable numbers of MDA-MD-231 human breast cancer cells, DU145 human prostate cancer cells, and AGS human gastric cancer cells decreased by 18, 31, and 69%, respectively, following treatment with 200 µg/mL CPE for 24 hr. CPE at various concentrations (0-200 µg/mL) markedly decreased AGS cell viability and increased apoptotic cell death dose and time dependently. CPE increased the levels of cleaved caspase-8, -7, -3, and poly (ADP-ribose) polymerase in a dose-dependent manner but not cleaved caspase-9. CPE exerted no effects on Bcl-2 and Bax levels. The level of X-linked inhibitor of apoptosis protein decreased within a narrow range following CPE treatment. The levels of Trail, DR4, and Fas-L increased dose-dependently in CPE-treated AGS cells. These results show that CPE decreases growth and induces apoptosis in AGS gastric cancer cells and that activation of the death receptor pathway contributes to CPE-induced apoptosis in AGS cells. In conclusion, CPE had more of an effect on gastric cancer cells than breast or prostate cancer cells, suggesting that chestnuts would have a positive effect against gastric cancer.

  18. Third molar development: measurements versus scores as age predictor.

    Science.gov (United States)

    Thevissen, P W; Fieuws, S; Willems, G

    2011-10-01

    Human third molar development is widely used to predict chronological age of sub adult individuals with unknown or doubted age. For these predictions, classically, the radiologically observed third molar growth and maturation is registered using a staging and related scoring technique. Measures of lengths and widths of the developing wisdom tooth and its adjacent second molar can be considered as an alternative registration. The aim of this study was to verify relations between mandibular third molar developmental stages or measurements of mandibular second molar and third molars and age. Age related performance of stages and measurements were compared to assess if measurements added information to age predictions from third molar formation stage. The sample was 340 orthopantomograms (170 females, 170 males) of individuals homogenously distributed in age between 7 and 24 years. Mandibular lower right, third and second molars, were staged following Gleiser and Hunt, length and width measurements were registered, and various ratios of these measurements were calculated. Univariable regression models with age as response and third molar stage, measurements and ratios of second and third molars as predictors, were considered. Multivariable regression models assessed if measurements or ratios added information to age prediction from third molar stage. Coefficients of determination (R(2)) and root mean squared errors (RMSE) obtained from all regression models were compared. The univariable regression model using stages as predictor yielded most accurate age predictions (males: R(2) 0.85, RMSE between 0.85 and 1.22 year; females: R(2) 0.77, RMSE between 1.19 and 2.11 year) compared to all models including measurements and ratios. The multivariable regression models indicated that measurements and ratios added no clinical relevant information to the age prediction from third molar stage. Ratios and measurements of second and third molars are less accurate age predictors

  19. 术前单独咨询对第三磨牙拔除焦虑水平的影响%Effect of Separate Consultation before Surgery on the Level of Anxiety in the Third Molar Extraction

    Institute of Scientific and Technical Information of China (English)

    任迅; 彭敏

    2016-01-01

    目的:评价术前单独咨询对第三磨牙拔除焦虑水平的影响。方法52名患者随机纳入试验组与对照组,每组26例。试验组患者在拔牙术前一周接受拔除第三磨牙相关信息的单独咨询,对照组在术前即时接受相同咨询。两组患者均填写焦虑与疼痛问卷,医师填写患者依从性和疗效评估量表,对结果进行统计分析。结果患者焦虑水平与拔牙后疼痛程度和治疗效果间存在明显相关。试验组患者在焦虑和疼痛程度上显著低于对照组,试验组患者对单独咨询接受度高,且对于麻醉和术中操作的依从性好,整体治疗效果好于对照组,差异均有统计学意义(P<0.05)。结论口腔医生可考虑对拔牙患者进行术前单独咨询。%Objective To evaluate the effect of separate consultation before surgery on the level of anxiety in the third molar extraction. Methods 52 patients were randomly divided into the experimental group and the control group,26 cases in each group. Patients in the experimental group were received the individual consultation on the relevant information of the third molar ex-traction a week before the extraction,while the control group were received the same advice immediately before surgery. The two groups of patients filled with anxiety and pain questionnaire and the doctors filled out the patient compliance and therapeutic effect assessment scale. The results were statistically analyzed. Results There was a significant correlation between the level of anxiety and pain after tooth extraction and treatment effect. The pain and anxiety of patients in the experimental group were significantly lower than that of the control group. Patients in the experimental group received a high degree of acceptance of separate consulta-tion,the compliance of anesthesia and surgery was good,and the overall treatment effect was better than that of the control group. And all of the differences were

  20. Programa de extrações seriadas: variáveis relacionadas com a extração de pré-molares Serial extraction: variables associated to the extraction of premolars

    OpenAIRE

    Tulio Silva Lara; Cibelle Cristina Oliveira dos Santos; Omar Gabriel da Silva Filho; Daniela Gamba Garib; Francisco Antônio Bertoz

    2011-01-01

    OBJETIVO: verificar o percentual de pacientes que necessitaram extração de dentes permanentes, pré-molares, dentre aqueles tratados com extração de dentes decíduos para correção do apinhamento primário na dentição mista, bem como analisar as possíveis variáveis relacionadas. MÉTODOS: a amostra foi composta por documentações ortodônticas de 70 pacientes na dentição permanente, cujo tratamento iniciou-se na dentição mista com planejamento de um programa de extrações seriadas (PES). Todos os pro...

  1. Metabolic fate of extracted glucose in normal human myocardium.

    OpenAIRE

    Wisneski, J A; Gertz, E W; Neese, R A; Gruenke, L D; D. L. Morris; Craig, J. C.

    1985-01-01

    Glucose is an important substrate for myocardial metabolism. This study was designed to determine the effect of circulating metabolic substrates on myocardial glucose extraction and to determine the metabolic fate of glucose in normal human myocardium. Coronary sinus and arterial catheters were placed in 23 healthy male volunteers. [6-14C]Glucose was infused as a tracer in 10 subjects. [6-14C]Glucose and [U-13C]lactate were simultaneously infused in the other 13 subjects. Simultaneous blood s...

  2. MicroCT study on the enamel mineral density of primary molars

    NARCIS (Netherlands)

    Elfrink, M.E.C.; Kalin, K.; van Ruijven, L.J.; ten Cate, J.M.; Veerkamp, J.S.J.

    2016-01-01

    Aim The aim of this study is to report on the mineral density of the enamel of primary molars related to the age of the child and to compare the mineral density of sound and carious enamel in those molars. Materials and methods This study included 23 children and 41 extracted primary molars. The

  3. An oral retractor designed for extraction of impacted mandibular third molar using turbine drilling method%自制拉钩在涡轮钻拔除下颌阻生智齿中的应用

    Institute of Scientific and Technical Information of China (English)

    黄伟; 李祖兵; 陈永吉; 李薇; 冷卫东

    2011-01-01

    During the extraction of impacted mandibular third molar using turbine drilling method, buccal tissue and distal flap likely to be embroiled by drill or damaged by the impact of air, causing complications such as postoperative swelling and pain. An oral retractor was designed and remade from local dental tray by author. The retractor significantly reduced the surgical trauma and complications. It can isolate distal and buccal soft tissue from the turbine drill, it is superior to the traditional cheek retractors.%涡轮钻法因创伤较小已逐渐取代传统的锤凿法在下颌智齿拔除术中得到提倡和应用.但使用涡轮钻时智齿远中和颊侧的软组织易卷入车针或受气流冲击造成损伤,引起术后肿胀、疼痛等并发症.为此,笔者将口腔局部托盘改装成拉钩,与传统的颊部拉钩相比可将涡轮钻与远中、颊侧软组织完全隔离,以保护软组织避免损伤.临床应用取得了较好的效果.

  4. Effect of serrapeptase on postoperative complication of mandibular complicated third molar extraction%舍雷肽酶对预防复杂下颌阻生智齿拔除术后并发症的疗效观察

    Institute of Scientific and Technical Information of China (English)

    刘奕; 林松杉; 章禾; 周明; 杨瑞华

    2012-01-01

    目的:观察应用舍雷肽酶预防下颌复杂阻生智齿拔除术后并发症的疗效.方法:选取临床需拔除复杂下颌阻生智齿的患者60例,随机分为实验组和对照组,各30例.实验组术后给予舍雷肽酶肠溶片10mg,tid,7d和甲硝唑片0.2,tid,3d,阿莫西林胶囊0.5,tid,3d.对照组仅给予甲硝唑片0.2,tid,3d,阿莫西林胶囊0.5,tid,3d作为预防性抗感染治疗.观察两组术后第1、2、7d的局部肿胀,疼痛及张口受限情况,并作统计学分析.结果:术后第2天实验组的局部肿胀程度为(9.17±4.17)mm,明显低于对照组(13.97±6.10)mm,差异有统计学意义(P<0.01);实验组的疼痛评分2.32±0.98与对照组3.50±1.26相比差异有统计学意义(P<0.01).张口受限情况两组间差异无统计学意义(P>0.05).结论:舍雷肽酶对于减轻下颌阻生智齿拔除术后疼痛与肿胀反应具有明显疗效.%Objective: To investigate the ability of serrapeptase to reduce postoperative swelling, pain and trismus after mandibular complicated wisdom teeth extraction. Methods: Sixty patients underwent impacted mandibular third molar extraction were involved and randomly divided into the treatment group which was given serrapeptase 1 0mg, tid, 7d and the control group which was given no serrapeptase. All the patients were given amoxicillin 0.5, tid, 3d and metronidazole 0.2, tid, 3d to prevent infection. The extent of check swelling, pain and interincisal distance were observed and measured on the 1st, 2nd and 7th postoperative days. Results: On the 2nd day after the tooth extraction, the value of swelling in treatment group(9.17± 4.17)mm was obviously lower than that of control group (13.97± 6.10) mm, with statistically significant difference (P0.05). Conclusion: The use of serrapeptase was effective to depress the postoperative negative reactions of the mandibular complicated third molar extraction.

  5. A preliminary study on 3D reconstruction and extracting mandibular first molar by dint of high-speed air turbine handpiece based on rapid prototyping%数控拔牙技术的实验研究

    Institute of Scientific and Technical Information of China (English)

    刘小明; 李健俫; 王建民; 张志光; 陈隆福; 陈贵丰; 黄湘穗

    2012-01-01

    目的 探讨牙颌数字化三维重建技术与快速成型技术、数控技术相结合,实现微创和自动化拔牙的可行性.方法 锥形束CT(cone beam computed tomography,CBCT)扫描下颌牙颌标本,获得数据.采用Mimics软件重建牙颌三维实体模型,Magics软件进行数据修补.选取左侧下颌第一磨牙三维立体数据,快速成型软件切片、UG软件数据转换,导入自行研制的数控拔牙机,严格坐标定位后磨除左侧下颌第一磨牙.结果 建立了良好的三维牙颌模型,用数控拔牙机成功磨除左侧下颌第一磨牙.结论 牙颌数字化三维重建技术与快速成型技术、数控技术相结合,应用于数控拔牙机拔除标本牙是可行的.%Objective A new technique combined with digital dental teeth, 3D reconstruction from cone beam computed tomography ( CBCT) data and rapid prototyping is presented, in order to solve the difficulties of tooth extracting by traditional chisel. Methods Software Mimics and Magics were used to reconstruct three dimensional model from CT image scanned by DCTPRo CBCT with 0.2 mm slice thickness. The achievement can be applied to accomplish tooth extracting by high-speed air turbine handpiece based on rapid prototyping. Results Mandibular first molar was extracted by dint of high-speed air turbine handpiece based on rapid prototyping. Conclusion The new technique combined with digital dental teeth and rapid prototyping can be used to solve the difficulties of the tooth extracting by traditional chisel.

  6. Histopathological and Immunohistochemical Study of the Discal Remodelling of Temporomandibular Joint Caused by Extraction of Molars%后牙缺失引起关节盘改建的组织学及免疫组化的研究

    Institute of Scientific and Technical Information of China (English)

    毛勇; 何惠明; 段小红

    2001-01-01

    Objective:To determine the characteristic of cells andextracellular matrix of disc of temporomandibular joint (TMJ) after loss of teeth.Methods:The left lower molars of adult rabbits were extracted,and immunohistochemical methods were used to determine the localization and quantities change of type Ⅰ and type Ⅱ and type Ⅲ collagen in the disc of both side after two weeks,one month and three months.Results:The disc of the non-extraction side became fibrosis,calcified,vacularized and deformed;while the opposite disc only showed calcified and disoriented of the fiber.The expression of type Ⅰ and Ⅱ collagen decreased after the extraction of the teeth,furthermore type Ⅰ collagen distributed unevenly.The immunostaining for type Ⅲ collagen was stronger than the control group.All these changes were more obvious in the disc of non-extraction side than in the opposite disc.Conclusion:The resistance and elasticity of disc in adult rabbits would impair after loss of the teeth for a long time, and the pathological changes of disc in the masticatory side were more obvious.%目的:研究一侧后牙缺失后双侧颞下颌关节关节盘细胞及细胞外基质的变化规律。方法:拔除成年家兔左侧下颌磨牙,用免疫组化方法检测Ⅰ、Ⅱ、Ⅲ型胶原在术后2周、1个月和3个月双侧关节盘的分布及含量变化。结果:非拔牙侧关节盘出现钙化、纤维化、血管侵入、形变等,而拔牙侧只有钙化、纤维走行改变;双侧Ⅰ、Ⅱ型胶原的表达均明显减少,并且Ⅰ型胶原出现不均衡分布;Ⅲ型胶原的表达术后明显增多,以非拔牙侧更明显。结论:后牙缺失过久会导致成年家兔关节盘抗压抗拉特性下降,脆性增加;单侧后牙缺失时,有牙侧关节盘的病变更显著。

  7. Immunocytochemical localization of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 of the human deciduous molar tooth germ development in the human fetus.

    Science.gov (United States)

    Miwa, Yoko; Fujita, Toshiya; Sunohara, Masataka; Sato, Iwao

    2008-01-01

    Vascular endothelial growth factor (VEGF) is a key regulator of blood vessel endothelial development. We used immunohistochemical methods to demonstrate the localization of VEGF and its receptors, showing the specific expression pattern of VEGF and VEGF receptor in the human deciduous tooth from the cap to late bell stages in the human fetus. Immunoreactivity to VEGF and its receptor VEGF receptor-2 (VEGFR-2) was intensely positive in the inner enamel epithelium at the cap stage and ranged from negative to moderately positive in the bell stage. At the late bell stage, VEGF immunoreactivity was mainly positive but weak for VEGFR-2. The intensity of VEGF and VEGFR-2 in odontoblasts increases from cap stage to late bell stage. We postulate that the dissimilar expression of VEGF in inner enamel epithelium, ameloblast and odontoblast during each stage of human tooth development may affect tooth germ formation.

  8. Distal caries of the second molar in the presence of a mandibular third molar - a prevention protocol.

    Science.gov (United States)

    Toedtling, V; Coulthard, P; Thackray, G

    2016-09-23

    Objectives The objectives of the prospective study were to establish the prevalence of distal caries (DC) in the mandibular second molar and to assess the outcomes of these diseased teeth in our population. Further aims were to identify associated risk factors and to design a protocol for prevention.Methods Clinical and radiographic data from 210 consecutive patients were ascertained over a three-month period. The sample population included all patients who had been referred to a hospital oral surgery department for a lower wisdom tooth assessment.Results A total of 224 mandibular third molars were included and assessed. The prevalence of caries affecting the distal aspect of the second molar was 38% (n = 85) in this population. In 18% of patients there was evidence of early enamel caries. Fifty-eight percent of caries was managed with restorative treatment but 11% of patients required second molar extraction and 13% of patients required the removal of the second and third molars. The prevalence of distal caries was significantly higher in patients with partially erupted wisdom teeth positioned below the amelocemental junction (P molar and in patients who presented with mesioangular impactions (P molars are useful disease predictors which can be used to indicate the likelihood of a caries process occurring on the distal aspect of the second mandibular molar. If patients' third molar teeth are not removed then consideration needs to be given to prevention and regular monitoring.

  9. Extraction of Natural Antioxidants from the Thelephora ganbajun Mushroom by an Ultrasound-Assisted Extraction Technique and Evaluation of Antiproliferative Activity of the Extract against Human Cancer Cells.

    Science.gov (United States)

    Xu, Dong-Ping; Zheng, Jie; Zhou, Yue; Li, Ya; Li, Sha; Li, Hua-Bin

    2016-10-01

    The Thelephora ganbajun mushroom has been found to be a potential rich source of natural antioxidants. In this study, an ultrasound-assisted extraction (UAE) technique together with GRAS (generally recognized as safe) solvents (ethanol and water) was used to maximize the extraction of antioxidants from Thelephora ganbajun. Five extraction parameters (ethanol concentration, solvent to solid ratio, extraction time, temperature and ultrasound power) were investigated by single-factor experiments, and then a central composite rotatable design was employed to study interaction of three key extraction parameters. The optimum conditions were as follows: 57.38% ethanol, 70.15 mL/g solvent to solid ratio, 10.58 min extraction time, 40 °C extraction temperature and 500 W ultrasound power. Under the optimum conditions, the antioxidant activity obtained was 346.98 ± 12.19 µmol Trolox/g DW, in accordance with the predicted value of 344.67 µmol Trolox/g DW. Comparison of UAE with conventional maceration and Soxhlet extraction, the UAE method showed stronger extract efficiency in a shorter extraction time. These results showed that UAE was an effective technique to extract antioxidants from Thelephora ganbajun. Furthermore, the extracts obtained under the optimized conditions exhibited antiproliferative activities toward human lung (A549), breast (MCF-7), liver (HepG2) and colon (HT-29) cancer cells, especially for liver and lung cancer cells. In addition, rutin, 2-hydrocinnamic acid and epicatechin were identified in the extract, which might contribute to antioxidant and antiproliferative activities.

  10. Extraction of Impacted Mandibular Third Molars Using Hydrogen Peroxide Preventive Effect of Dry Socket Observation%拔除下颌阻生智齿使用双氧水预防干槽症的疗效观察

    Institute of Scientific and Technical Information of China (English)

    李志升

    2014-01-01

    目的观察3%双氧水在拔除下颌阻生智齿中预防干槽症的临床效果。方法随机应用于需要拔除的下颌阻生智齿240颗。实验组拔牙后创面用干槽症发生率为1.67%,非实验组为5.83%。两组干槽症发生率统计学分析显著性差异(约0.05)。结果实验组120例发生干槽症2例发生率为1.67%。对照组发生干槽症7例发生率为5.83%。结论拔除下颌阻生智齿使用3%的双氧水冲洗能有效预防发生干槽症。同时防止感染,注意口内卫生等是防止发生干槽症的关键。%Objective To observe the clinical ef ect of 3% hydrogen peroxide in the prevention of dry socket impacted mandibular wisdom teeth in the. Methods Randomly used in extraction of impacted mandibular impacted wisdom teeth need 240. The experimental group after tooth extraction wound dry socket incidence rate was 1.67%, the non experimental group 5.83%. The two groups the incidence of dry socket statistical y significant difference ( <0.05). Results The experimental group 120 cases of dry socket incidence was 1.67% in 2 cases. Group of dry socket incidence was 5.83% in 7 cases. Conclusion The extraction of impacted mandibular third molars with 3% hydrogen peroxide can ef ectively prevent the dry socket. At the same time, prevent infection, pay attention to oral hygiene is the key to prevent the occurrence of dry socket.

  11. Management of third molar teeth from an endodontic perspective

    Directory of Open Access Journals (Sweden)

    Hany Mohamed Aly Ahmed

    2012-01-01

    Full Text Available Third molar teeth are subject to many dental complications because of their most posterior location, aberrant occlusal anatomy and abnormal eruption patterns. Owing to these anatomical limitations, their extraction remains the treatment of choice for many dental practitioners. Despite being a common dental procedure, minimum intervention and retaining every functional component of the dental arch are of prime importance in contemporary dental practice. As such, this review aims to discuss the application of this conservative approach on third molar teeth from an endodontic perspective. The internal and external root anatomy of maxillary and mandibular third molars and their relation to the surrounding vital structures are described. These anatomical landmarks are then correlated to the decision making for endodontic treatment strategies of third molars. In addition, the recommended guidelines that should be followed while commencing endodontic treatment in third molars are outlined.

  12. Outcomes of impacted mandibular third molar extraction with different tooth separation techniques%不同分牙方式拔除低位阻生下颌第三磨牙的临床效果分析

    Institute of Scientific and Technical Information of China (English)

    董建辉; 朱亚琴

    2011-01-01

    Objective To observe and compare the outcomes of impacted mandibular third molar extraction with different tooth separation techniques. Methods One hundred and fifty low and medial impacted mandibular third molars in 150 patients were selected, and were extracted with traditional hammer and chisel technique ( n = 50), hammer and chisel + "T"typed tooth separation technique ( n = 50) and turbine + "T" typed tooth separation technique ( n = 50), respectively. The incidences of postoperative reactions ( regional swelling and pain) and complications were compared among groups. Results The incidence of severe postoperative regional swelling in patients treated with turbine + "T" typed tooth separation technique was significantly lower than that in those treated with traditional hammer and chisel technique (16% vs 34%, P < 0.05).The incidences of postoperative Ⅲ degree pain in patients with traditional hammer and chisel technique, hammer and chisel +"T" typed tooth separation technique and turbine + "T" typed tooth separation technique were 36%, 20% and 4%,respectively, and there were significant differences among patients treated with different techniques ( P < 0. 05). The incidences of postoperative complications such as numbness of lower lip, extraction of lorum together with dental root and fever in patients treated with hammer and chisel + "T" typed tooth separation technique and turbine + "T" typed tooth separation technique were significantly lower than those in patients treated with traditional hammer and chisel technique (P < 0.05). Conclusion Compared with traditional hammer and chisel technique, turbine + "T" typed tooth separation technique works better in extraction of impacted mandibular third molars with less postoperative reactions and complications.%目的 观察和比较以不同分牙方式拔除低位阻生下颌第三磨牙的临床效果.方法 选取第三磨牙低位水平、近中阻生或完全骨埋伏阻生的150例患者作

  13. Cortisol extraction through human skin by reverse iontophoresis.

    Science.gov (United States)

    Ventura, Stephanie A; Heikenfeld, Jason; Brooks, Tiffany; Esfandiari, Leyla; Boyce, Steven; Park, Yoonjee; Kasting, Gerald B

    2017-04-01

    Continuous monitoring of cortisol at the surface of the skin would advance the diagnosis and treatment of cortisol-related diseases, or of elevated cortisol levels related to stress in otherwise healthy populations. Reliable and accurate detection of cortisol at the skin surface remains a limiting factor in real-time monitoring of cortisol. To address this limitation, cortisol extraction through excised human skin by reverse iontophoresis was studied in vitro in side-by-side diffusion cells using a radiolabeled probe. The skin was subjected to four direct current regimens (0, 28, 56, 113μAcm(-2)) with the anode in the donor chamber and the cumulative cortisol concentrations recorded in the receiver chamber. The 56 and 113μAcm(-2) regimens significantly increased transport of (3)H-cortisol through the skin, and current density correlated directly with transcutaneous transport of (3)H-cortisol. The threshold of detection of electroosmotic versus passive diffusion of cortisol through the skin was between 28 and 56μAcm(-2). The results of this study are significant in examining how lipophilic analytes found in the bloodstream respond to reverse iontophoresis across the skin. In addition, a device integration technique is presented which illustrates how continuous cortisol extraction and sensing could potentially be achieved in a conventional wearable format.

  14. An Improved AAM Method for Extracting Human Facial Features

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    Tao Zhou

    2012-01-01

    Full Text Available Active appearance model is a statistically parametrical model, which is widely used to extract human facial features and recognition. However, intensity values used in original AAM cannot provide enough information for image texture, which will lead to a larger error or a failure fitting of AAM. In order to overcome these defects and improve the fitting performance of AAM model, an improved texture representation is proposed in this paper. Firstly, translation invariant wavelet transform is performed on face images and then image structure is represented using the measure which is obtained by fusing the low-frequency coefficients with edge intensity. Experimental results show that the improved algorithm can increase the accuracy of the AAM fitting and express more information for structures of edge and texture.

  15. Pharmacokinetic Comparison of Soy Isoflavone Extracts in Human Plasma.

    Science.gov (United States)

    Rodríguez-Morató, Jose; Farré, Magí; Pérez-Mañá, Clara; Papaseit, Esther; Martínez-Riera, Roser; de la Torre, Rafael; Pizarro, Nieves

    2015-08-12

    The soy isoflavones daidzein and genistein produce several biological activities related to health benefits. A number of isoflavone extracts are commercially available, but there is little information concerning the specific isoflavone content of these products or differences in their bioavailability and pharmacokinetics. This study describes the development and validation of an analytical method to detect and quantify daidzein, genistein, and equol in human plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The method was applied in a crossover, randomized, bioavailability study. Twelve healthy volunteers were administered the same total isoflavones dose from two isoflavone supplement preparations (Super-Absorbable Soy Isoflavones (Life Extension, USA) and Fitoladius (Merck, Spain)). The pharmacokinetic parameters (AUC0-24/dose and Cmax/dose) of the isoflavones from the two preparations differed significantly. Such differences in bioavailability and kinetics may have relevant effects on the health benefits derived from their intake.

  16. Surgical approach to impacted mandibular third molars--operative classification.

    Science.gov (United States)

    Abu-El Naaj, Imad; Braun, Refael; Leiser, Yoav; Peled, Micha

    2010-03-01

    The aim of the present study is to suggest a convenient way to classify the position of the impacted third mandibular molar relative to the mandibular canal and to suggest indications for the use of each surgical approach for mandibular third molar extraction. The presented new typing system, Third Molar Classification (TMC), is a simple and easy-to-apply method for the surgical management of mandibular third molars and can be extended for any ectopic or impacted mandibular tooth. There are 3 major types of third molar positions. The second type is subdivided further into 2 subtypes. In the present study, 9 patients with high-risk mandibular third molars were treated according to the present classification and are presented and discussed. Patients typed as TMC IIb were treated with a sagittal split osteotomy approach and patients typed as TMC III were treated with an extraoral approach. The operative classification was successfully implemented in very rare cases of deeply impacted mandibular third molars. In 3 of 9 cases (33%) minor complications included some degree of hypoesthesia using the extraoral approach; these complications resolved spontaneously without the need for any intervention. The present study describes the use of a new surgical classification system for treatment planning in all types of mandibular third molar extractions. We believe that the present classification could help the oral and maxillofacial surgeon in decision-making and limit the possible risks that are present when attempting to extract impacted mandibular third molars. Copyright (c) 2010 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  17. Permanent molar pulpotomy with a new endodontic cement: A case series

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    Asgary Saeed

    2009-01-01

    Full Text Available The aim of this case series was to determine the clinical and radiographic success rate of pulpotomy, with new endodontic cement (NEC, in human mature permanent molar teeth. Twelve molars with established irreversible pulpitis were selected from patients 14 - 62 years old. The selection criteria included carious pulp exposure with a positive history of lingering pain. After isolation, caries removal, and pulp exposure, pulpotomy with NEC was performed and a permanent restoration was immediately placed. At the first recall (+1 day no patients reported postoperative pain. One wisdom tooth had been extracted after two months because of failure in coronal restoration. Eleven patients were available for the second recall, with a mean time of 15.8 months. Clinical and radiographic examination revealed that all teeth were functional and free of signs and symptoms. Histological examination of the extracted teeth revealed complete dentin bridge formation and a normal pulp. Although the results favored the use of NEC, more studies with larger samples and a longer recall period were suggested, to justify the use of this novel material for treatment of irreversible pulpitis in human permanent molar teeth.

  18. MANAGEMENT OF MANDIBULAR THIRD MOLAR SURGERY TO PRESERVE PERIODONTAL HEALTH OF SECOND MOLAR

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    Elitsa G. Deliverska

    2017-06-01

    Full Text Available Background: Extraction of impacted third molar violates surrounding soft and bony tissues. Various surgical approaches and surgical technics have an important impact on the periodontal health of the adjacent second molar. Purpose: The aim of this review is to analyse the causes that can affect postoperative periodontal outcomes for the mandibular second molars (LM2 adjacent to the impacted/ semi impacted mandibular third molars (LM3. Material and Methods: Electronic searches were conducted through the MEDLINE (PubMed, Scopus, etc. databases to screen all relevant articles published from inception to April 2017. Results: Different flap techniques had no significant impact on the probing depth reduction or on the clinical attachment level of LM2. Szmyd and paramarginal flap designs may be the most effective in reducing the probing depth after third molar surgery, and the envelope flap may be the least effective. Use of bone substitutes and guided tissue regeneration therapy has been proposed, to eliminate or prevent these periodontal defects, but there is still no consensus on their predictability or clinical benefit. Higher costs and the risk of postoperative inflammatory complications should also be taken into proper account, as with any surgical procedure. “Orthodontic extraction” is indicated for that impacted M3 that present a high risk of postoperative periodontal defects at the distal aspect of adjacent M2. Conclusion: Risk factors associated with bone loss following lower third molar extraction includes age, the direction of the eruption, preoperative bone defects, and resorbtion of the LM2 root surface. Prevention of such periodontal defects continues to challenge clinicians.

  19. External root resorption of the second molar associated with third molar impaction: comparison of panoramic radiography and cone beam computed tomography.

    Science.gov (United States)

    Oenning, Anne Caroline Costa; Neves, Frederico Sampaio; Alencar, Phillipe Nogueira Barbosa; Prado, Rodrigo Freire; Groppo, Francisco Carlos; Haiter-Neto, Francisco

    2014-08-01

    The aim of the present study was to compare panoramic radiography and cone beam computed tomography (CBCT) for the assessment of external root resorption (ERR) of second molars associated with impacted third molars. In addition, the prevalence of ERR in second molars and the inclinations of the third molars more associated with ERR were investigated in both imaging methods. The sample consisted of 66 individuals with maxillary and mandibular impacted third molars (n = 188) seen on panoramic radiographs and CBCT images. The presence of ERR on the adjacent second molar was investigated, and the position of the third molar was determined using Winter's classification (vertical, horizontal, mesioangular, distoangular, and transverse). Statistical analysis was performed using the χ(2) test, Fisher exact test, and 2-proportion Z test (the significance level was set at 5%). A significantly greater number of cases of ERR (P third molars in mesioangular and horizontal inclinations were more likely to cause resorption of the adjacent teeth. CBCT should be indicated for the diagnosis of ERR in second molars when direct contact between the mandibular second and third molars has been observed on panoramic radiographs, especially in mesioangular or horizontal impactions. Furthermore, considering the propensity of these teeth to cause ERR in second molars, third molar prophylactic extraction could be suggested. Copyright © 2014 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  20. Molar Uprighting Using Mini-Screws after Distalization by the Pendulum Appliance: A Case Report

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    Amir Moradi

    2013-12-01

    Full Text Available A 16-year-old Class II female patient was treated without tooth extraction. The upper first molars were distalized by the Pendulum appliance. After six months, the molars tipped significantly to the distal. To correct this side effect, we decided to upright the molars using skeletal anchorage. On each side, a mini-screw was inserted between first and second premolars in the buccal cortical plate. An auxiliary spring was placed between the mini-screw head and the molar buccal tube. The resultant moment made the first molar upright. In addition, the side effects of this mechanic, i.e. molar intrusion and molar buccal tipping, counteract the extrusion and medial movement caused by the Pendulum Appliance. The aim of this case report was to present an innovative method for molar uprighting using skeletal anchorage.

  1. Molar Incisor Hypomineralization.

    Science.gov (United States)

    Rao, Murali H; Aluru, Srikanth C; Jayam, Cheranjeevi; Bandlapalli, Anila; Patel, Nikunj

    2016-07-01

    Molar incisor hypomineralization (MIH) is a developmental defect affecting teeth. High prevalence rates of MIH and its clinical implications are significant for both the patients and clinicians. A wide variation in defect prevalence (2.4-40.2%) is reported. It seems to differ with regions and various birth cohorts. Some of the recent prevalence studies are tabulated. Patient implications include hypersensitive teeth, rapid progression of caries, mastication impairment due to rapid attrition, and esthetic repercussions. Implications for clinicians include complexity in treatment planning and treatment implementation, poor prognosis of the restorations, difficulty in achieving pain control during treatment, and behavior management problems. Intention of this paper is to review the etio-pathogenesis, prevalence, clinical features, diagnostic features, and eventually present a sequential treatment approach, i.e., in accordance with current clinical practice guidelines.

  2. [The relation of pericoronitis to the position of the mandibular third molar].

    Science.gov (United States)

    Lee, D K; Kim, B J

    1989-02-01

    Pericoronitis is the most commonly encountered pathologic condition involving the mandibular third molar. Because of the dangers associated with mandibular third molar pericoronitis, prophylactic extraction of third molar at high risk has been recommended. We studied 411 patients with mandibular third molar pericoronitis by clinical symptoms and radiographic measurement of mandibular third molar height, wideth and angulation. The results were as follows: 1. Mandibular third molar pericoronitis is frequently seen in third decade and there are no sexual difference significantely. 2. In inflammatory type of mandibular third molar pericoronitis, chronic pericoronitis occured more frequently than acute type. 3. In relation to angulation and height, mandibular third molar most likely to be afflicted with pericoronitis is vertical eruption at occlusal plane of the second molar. 4. In relation to angulation and width, it appears that the position of the mandibular third molar most likely to be afflicted with pericoronitis is in a vertically erupted tooth of which the space between the ramus and the distal side of the second molar is less than the mesiodistal diameter of crown. (Class II). 5. In relation to height and width, it appears that the position of the mandibular third molar most likely to be afflicted with pericoronitis is class II width (described above)at occlusal plane of the second molar.

  3. A Clinical Observation of Col oidal Silver Gelatin Sponge on Preventing Dry Socket Caused by Extraction of Impacted Mandibular Third Molar%胶质银明胶海绵在下颌阻生智齿拔牙术后干槽症的临床观察

    Institute of Scientific and Technical Information of China (English)

    李明; 于素平

    2012-01-01

      目的观察胶质银止血明胶海绵预防阻生智齿拔牙后干槽症的效果。方法分组对256例下颌第三磨牙拔除术后进行不同的拔牙创口处理,观察术后发生干槽症的情况,分析病因。结果拔牙后创口常规处理发生干槽症的机率为11%,而创口置入胶质银止血明胶海绵,干槽症发生率1%。结论拔牙后创口用胶质银止血明胶海绵,可以大大减少干槽症发病机率。%  Objective To study the clinical effect of colloidal silver gelatin sponge on preventing dry socket of mandibular third molar extraction. Methods Group of 256 cases of impacted mandibular third molar extraction different after the extraction wound treatment, observation of dry socket occurred after the situation, analyze causes. Results Conventional extraction results after wound treatment of dry socket in the probability of 11%, and wound implantation colloidal silver gelatin sponge, dry socket incidence of 1%. Conclusion After extraction with colloidal silver gelatin sponge,which can greatly reduce the incidence rate of dry socket.

  4. Calendula extract: effects on mechanical parameters of human skin.

    Science.gov (United States)

    Akhtar, Naveed; Zaman, Shahiq Uz; Khan, Barkat Ali; Amir, Muhammad Naeem; Ebrahimzadeh, Muhammad Ali

    2011-01-01

    The aim of this study was to evaluate the effects of newly formulated topical cream of Calendula officinalis extract on the mechanical parameters of the skin by using the cutometer. The Cutometer 580 MPA is a device that is designed to measure the mechanical properties of the skin in response to the application of negative pressure. This non-invasive method can be useful for objective and quantitative investigation of age related changes in skin, skin elasticity, skin fatigue, skin hydration, and evaluation of the effects of cosmetic and antiaging topical products. Two creams (base and formulation) were prepared for the study. Both the creams were applied to the cheeks of 21 healthy human volunteers for a period of eight weeks. Every individual was asked to come on week 1, 2, 3, 4, 5, 6, 7, and 8 and measurements were taken by using Cutometer MPA 580 every week. Different mechanical parameters of the skin measured by the cutometer were; R0, R1, R2, R5, R6, R7, and R8. These were then evaluated statistically to measure the effects produced by these creams. Using ANOVA, and t-test it was found that R0, and R6 were significant (p 0.05). The instrumental measurements produced by formulation reflected significant improvements in hydration and firmness of skin.

  5. Extraction of Natural Antioxidants from the Thelephora ganbajun Mushroom by an Ultrasound-Assisted Extraction Technique and Evaluation of Antiproliferative Activity of the Extract against Human Cancer Cells

    Science.gov (United States)

    Xu, Dong-Ping; Zheng, Jie; Zhou, Yue; Li, Ya; Li, Sha; Li, Hua-Bin

    2016-01-01

    The Thelephora ganbajun mushroom has been found to be a potential rich source of natural antioxidants. In this study, an ultrasound-assisted extraction (UAE) technique together with GRAS (generally recognized as safe) solvents (ethanol and water) was used to maximize the extraction of antioxidants from Thelephora ganbajun. Five extraction parameters (ethanol concentration, solvent to solid ratio, extraction time, temperature and ultrasound power) were investigated by single-factor experiments, and then a central composite rotatable design was employed to study interaction of three key extraction parameters. The optimum conditions were as follows: 57.38% ethanol, 70.15 mL/g solvent to solid ratio, 10.58 min extraction time, 40 °C extraction temperature and 500 W ultrasound power. Under the optimum conditions, the antioxidant activity obtained was 346.98 ± 12.19 µmol Trolox/g DW, in accordance with the predicted value of 344.67 µmol Trolox/g DW. Comparison of UAE with conventional maceration and Soxhlet extraction, the UAE method showed stronger extract efficiency in a shorter extraction time. These results showed that UAE was an effective technique to extract antioxidants from Thelephora ganbajun. Furthermore, the extracts obtained under the optimized conditions exhibited antiproliferative activities toward human lung (A549), breast (MCF-7), liver (HepG2) and colon (HT-29) cancer cells, especially for liver and lung cancer cells. In addition, rutin, 2-hydrocinnamic acid and epicatechin were identified in the extract, which might contribute to antioxidant and antiproliferative activities. PMID:27706082

  6. Extraction of Natural Antioxidants from the Thelephora ganbajun Mushroom by an Ultrasound-Assisted Extraction Technique and Evaluation of Antiproliferative Activity of the Extract against Human Cancer Cells

    Directory of Open Access Journals (Sweden)

    Dong-Ping Xu

    2016-10-01

    Full Text Available The Thelephora ganbajun mushroom has been found to be a potential rich source of natural antioxidants. In this study, an ultrasound-assisted extraction (UAE technique together with GRAS (generally recognized as safe solvents (ethanol and water was used to maximize the extraction of antioxidants from Thelephora ganbajun. Five extraction parameters (ethanol concentration, solvent to solid ratio, extraction time, temperature and ultrasound power were investigated by single-factor experiments, and then a central composite rotatable design was employed to study interaction of three key extraction parameters. The optimum conditions were as follows: 57.38% ethanol, 70.15 mL/g solvent to solid ratio, 10.58 min extraction time, 40 °C extraction temperature and 500 W ultrasound power. Under the optimum conditions, the antioxidant activity obtained was 346.98 ± 12.19 µmol Trolox/g DW, in accordance with the predicted value of 344.67 µmol Trolox/g DW. Comparison of UAE with conventional maceration and Soxhlet extraction, the UAE method showed stronger extract efficiency in a shorter extraction time. These results showed that UAE was an effective technique to extract antioxidants from Thelephora ganbajun. Furthermore, the extracts obtained under the optimized conditions exhibited antiproliferative activities toward human lung (A549, breast (MCF-7, liver (HepG2 and colon (HT-29 cancer cells, especially for liver and lung cancer cells. In addition, rutin, 2-hydrocinnamic acid and epicatechin were identified in the extract, which might contribute to antioxidant and antiproliferative activities.

  7. Iatrogenic displacement of impacted third molar. Case report

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    Konstantinos TSIKLAKIS

    2013-08-01

    Full Text Available Peri-operative complications may occur during the surgical extraction of impacted mandibular third molars such as the iatrogenic displacement of the whole tooth or a tooth fragment in to the adjacent anatomical structures. The purpose of this case report is to present the diagnosis and treatment planning, as well as the surgical management required for the removal of a 3rd molar displaced in the soft tissues of the floor of the mouth. A 38-year old male patient presented to the Dental School, complaining of pain around the left submandibular area that started three months after the extraction of the impacted mandibular left third molar. At the radiographic examination the tooth was detected in the soft tissues of the floor of the mouth and in close proximity to the lingual plate. It is noteworthy that the patient was under the assumption that the tooth had been extracted successfully. Radiographically the tooth appeared at an 180° turnaround from its original position. Surgical extraction of the displaced third molar took place under local anaesthesia. No post-operative complications were reported. Thorough clinical and radiographic examination, as well as competency at surgical procedures are prerequisites for the appropriate surgical management of impacted third molars.

  8. [Comparing and evaluating six methods of extracting human genomic DNA from whole blood].

    Science.gov (United States)

    Chang, Jing-Jing; Zhang, Su-Hua; Li, Li

    2009-04-01

    Comparing the differences in purity and yield among six methods of extracting human genomic DNA from whole blood, which included Classic Phenol-chloroform extraction, modified combined technique composed of improved Phenol-chloroform extraction and Chelex-100 extraction, Chelex-100 extraction, IQ, Qiagen and SP. Ten samples of intravenous whole blood (5 mL/sample) were collected and human genomic DNA was extracted with these six methods. The purity and concentration of the DNA products were detected by ultraviolet spectrophotometry and fluorescent quantitation technique, the yield was calculated and tested with statistical software. The Chelex-100 extraction was inferior in DNA purity to other methods while the other five methods showed no statistical difference. Modified combined technique was the poorest and IQ was the best in yield among the six methods of extraction. Statistical result showed that the extraction with high quality kits was better than that with classic Phenol-chloroform extraction, Chelex-100 extraction and modified combined technique composed of improved Phenol-chloroform. There was statistical difference between them. Comparing to Phenol-chloroform extraction and Chelex-100 extraction, high quality kits are more useful in DNA extraction from forensic materials.

  9. The Effects in Extraction of Impacted Mandibular Third Molars with High-speed Turbine Drill a Meta-analysis%高速涡轮钻法拔除下颌阻生智齿术后效果meta分析

    Institute of Scientific and Technical Information of China (English)

    刘茁; 李璇; 李守宏; 庞光明

    2012-01-01

    目的:评价高速涡轮钻拔除下颌阻生智齿术后效果.方法:计算机检索1979-2012年CNKI、万方、维普、CBM中文数据库高速涡轮钻法拔除下颌阻生智齿的随机对照试验.采用cochrane协作网推荐的方法进行质量评价,对手术时间、术后疼痛、术后肿胀、张口受限、干槽症及颞颌关节疼痛等并发症进行系统分析,运用Revman 5.0 Tutorial软件分析,计算比值比(OR)或均值差(MD)和95%可信区间(CI).结果:本研究共纳入高速涡轮钻法1313例和传统法1314例,涡轮钻法手术时间MD值为-15.24 min(95%CI =-21.12-9.35);涡轮钻法Ⅰ、Ⅱ、Ⅲ度疼痛OR值分别为2.31、0.45、0.36(95% CI=1.37-3.92,95%CI =0.37-0.55,95%CI=0.36-0.61);涡轮钻法Ⅰ、Ⅱ、Ⅲ度肿胀OR值分别为1.36、1.11、0.33(95%CI =0.53-3.51,95%CI =0.47-2.61,95%CI =0.23-0.48);涡轮钻法Ⅰ、Ⅱ、Ⅲ度张口受限OR值分别为2.11、0.38、0.17(95%CI =1.08-4.10,95%CI =0.30-0.48,95%CI =0.11-0.26);涡轮钻法干槽症OR值为0.29(95%CI =0.21-0.41);涡轮钻法术后颞下颌关节疼痛发生率低或无.结论:高速涡轮钻拔牙法能缩短拔牙时间,降低术后疼痛、肿胀、张口受限、干槽症和颞下颌关节疼痛发生率.%Objective: To evaluate of the effects in extraction of impacted mandibular third molars with Highspeed turbine drill. Methods: The CNKK1979 -2012) , CIP ,wanfang and CBM library database were searched and supplemented by review of conference proceedings and publication bibliographies. All original single institution studies reporting outcomes in extraction of impacted mandibular third molars with High-speed turbine drill(HSTD) were considered. Odds ratios (OR) and 95% confidence intervals (CD based on random effects models were calculated. Results: Fifteen publications, all RCT studies, involving 1313 HSTD and 1314 traditional approach, were included. The MD of Operation time of HSTD was -15. 24 minutes (95% CI = -21. 12-9. 35

  10. An assessment on the effects of mandibular impacted third molar surgery on the periodontium of the adjacent molar

    Directory of Open Access Journals (Sweden)

    Ramezanian M

    2003-06-01

    Full Text Available Statement of Problem: One of the important indications for the extraction of mandibular impacted third"nmolar is to preserve the periodontal health of the adjacent second molar from the destructive effects of"nplaque accumulation, acute and chronic inflammation."nAim: The purpose of this study was to determine the effect of mandibular third molar surgery on the"nPDL of the adjacent molar."nMaterials and Methods: Thirty patients, referred to the department of maxillofacial surgery, Tehran"nUniversity of Medical Sciences, were selected, based on the desirable conditions. They had no history of"nsystemic diseases and their adjacent molar was healthy. Probing depth (PD at seven points and"nattachment level (AL at three points were measured preoperatively. Standardized periapical"nradiographics were taken regularly for all patients. All the above-mentioned procedures repeated after"nthree months of surgery. Findings were analyzed by Paired t-test."nResults: A significant difference in probing depth (PD was observed before and after surgery, meaning"nthat probing depth decreased after mandibular third molar surgery. Attachment level was also decreased"npost operatively; moreover. Intrabony defects (IBD showed healing after surgery."nConclusion: Considering the limitations of the present study, impacted third molar surgery is suggested"nto prevent periodontal problems of the adjacent molar.

  11. Antibiotic therapy in impacted third molar surgery.

    Science.gov (United States)

    Monaco, G; Staffolani, C; Gatto, M R; Checchi, L

    1999-12-01

    The use of routine antibiotic therapy in patients undergoing surgical third molar extraction is controversial. The efficacy of antibiotic therapy in preventing postoperative complications following surgical third molar extractions was evaluated in 141 patients. In the test group (66 patients), the protocol utilized a regimen of 2 g of amoxicillin orally daily for 5 d postoperatively, starting at the completion of surgery. In the control group (75 patients), no antibiotic therapy was given. No significant difference was found between the test group and the control group in the incidence of postoperative sequelae, i.e. fever, pain, swelling and alveolar osteitis. A statistically significant association between smoking, habitual drinking and increased postoperative pain and fever was found. Patient age > or = 18 yr was positively correlated with an increased incidence of alveolar osteitis. Swelling was found to be gender-related, in that female patients experienced more swelling than male patients. No correlation was found between the time required for surgery or difficulty of extraction and post-operative pain. In conclusion, no difference was found between patients receiving postoperative amoxicillin and the control group in the incidence of postoperative sequelae.

  12. In Vitro Effect of Ananas Comosus Extract on Human Sperm

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    Mélany Uribe-Clavijo

    2012-06-01

    Full Text Available Spermicides has been used for birth control, but its chemical components are harmful for the vaginal epithelium, thus other studies have been done, given the possibility to use different extracts from plants as spermicidal agents that avoid vaginal tissue damages. The aim of this study was to evaluate the Ananas comosus extract as spermicidal. Semen samples were incubated with pineapple extract, and motility and viability was evaluated after contact. In addition the cytotoxic effect was evaluated using MTS assay on HeLa cells. Motility (p<0.001 and viability (p<0.001 decreased after treatment with 50% dilution. However, extract had a cytotoxic effect on epithelial cell line. In conclusion, despite the cytotoxic effect in the same concentrations that spermicidal activity was observed, further studies determining the fractions of the extract have spermicidal properties are necessary.

  13. 第三磨牙拔除同期超声治疗对第二磨牙远中牙周组织的影响%Effect of lower third molar extraction with simultaneous scaling on periodontal tissue of the lower second molar

    Institute of Scientific and Technical Information of China (English)

    陈荣锐; 和璐

    2013-01-01

    Objective To invesligale the effect of lower third molar exlraclion with simultaneous scaling on periodonlal tissue of the lower second molar. Methods Forly-three palienls who underwent exlraclion of bilaleral mandibular mesioangular impacted ihird molars were included in this study. Digilal X-ray was taken and the alveolar bone heighl measured on the dislal side of the lower second molar before and 6 monlhs after exlraclion of the ihird molar. In the test sides, ullrasonic scaling was performed before and afler exlraclion, and in the conlrol sides the sockel was only rinsed wilh 0. 9% saline. Clinical parameters including plaque index(PLI) , bleeding index(BI) , periodonlal pockel deplh(PD) were recorded before and afler exlraclion. Results PD, BI and PLI of the dislal side of the lower second molar were significantly reduced ( P < 0. 001) six monlhs afler exlraclion compared wilh those before the exlraclion. The reduction of PD and gain of bone heighl were more apparent in the test sides than in the conlrol sides(P<0. 05). Conclusion The lower ihird molar exlraclion wilh simultaneous scaling was more beneficial to the recovery of the periodonlal lissue.%目的 观察第三磨牙(M3)拔除同期超声洁治和刮治对第二磨牙(M2)远中牙周指标的影响.方法采用自身对照的分口设计.选择要拔除下颌双侧近中倾斜阻生智牙的患者43人,随机分配其一侧为超声治疗侧,在拔牙前后对M2远中面进行超声洁治和刮治,另一侧则为对照侧,只对M2远中面在拔牙前后以0.9%生理盐水冲洗.记录拔牙前和拔牙后6个月M2远中的探诊深度、出血指数、菌斑指数,并测量拔牙前和拔牙后6个月X线片显示的M2近远中牙槽嵴顶到相应釉牙骨质界的距离.结果 M3拔除后6个月无论超声治疗侧还是对照侧,第二磨牙远中面的探诊深度、出血指数、菌斑指数都较拔牙前显著降低,有统计学差别(P<0.001).6个月后超声治疗侧比对照侧在探诊

  14. Inhibition of human low-density lipoprotein oxidation in vitro by ginger extracts.

    Science.gov (United States)

    Gunathilake, K D Prasanna P; Rupasinghe, H P Vasantha

    2014-04-01

    Oxidative modification of low-density lipoprotein (LDL) is thought to play a key role in atherosclerotic plaque formation. Currently, there is a renewed interest in ginger because of its antioxidants and cardioprotective properties. The effects of ethanol, methanol, ethyl acetate, and hexane solvent extracts of ginger and pure major ginger constituents on Cu(2+)-induced oxidation of human LDL in vitro were examined. The LDL oxidation inhibition by ethanol, methanol, ethyl acetate, and hexane extracts of ginger was 71%, 76%, 67%, and 67%, respectively, at their optimum extraction conditions. Inhibition of LDL oxidation by water extracts of ginger, which was prepared by ultrasonic-assisted extraction conditions of 52°C for 15 min, was about 43%. Phenolic bioactives of ginger-6-gingerols, 8-gingerols, 10-gingerols, and 6-shogaol-seem to be strong inhibitors of Cu(+2)-induced LDL oxidation. Overall, ginger extracts, including the water extract possess the antioxidant activities to inhibit human LDL oxidation in vitro.

  15. Gelatamp colloidal silver gelatin sponge can effectively prevent complications caused by extraction of impacted mandibular third molar%Gelatamp胶质银明胶海绵能有效预防下颌阻生智齿拔除术后并发症

    Institute of Scientific and Technical Information of China (English)

    万桂芹

    2011-01-01

    Objective:To observe effects of Gelatamp colloidal silver gelatin sponge in preventing complications caused by extraction of impacted mandibular third molar. Methods;Total 582 patients with impacted mandibular third molars were quasi-randomly assigned into two groups with 297 in the test group and 285 in the control group. In test group, Gelatamp colloidal silver gelatin sponge was put into the socket after the extraction of impacted mandibular third molar, while nothing was put into the socket after the extraction in control group. Patients visited at 0.5 h,3 d and 7 d after the extraction. Complications were observed and the incidence rate was calculated. Results;The incidence rate of postoperative complications in test group was lower than that in control group,and there were significant differences between the test group and the control group (P < 0.05) Conclusion;Gelatamp colloidal silver gelatin sponge can effectively prevent complications caused by extraction of impacted mandibular third molar. Therefore it is recommendable in clinical practice.%目的:观察Gelatamp胶质银明胶海绵预防下颌阻生智齿拔除术后并发症的效果.方法:选择需要行下颌阻生智齿拔除术的患者582例,按半随机方法分为2组,实验组297例,拔牙后清理干净牙槽窝,植入Gelatamp胶质银明胶海绵,咬无菌棉球30 min,常规口服头孢类药物3d;对照组285例,拔牙后牙槽窝内不放置任何填充物,其余同对照组.拔牙后嘱患者半小时、第3天、第7天复诊,按本研究评判标准判断两组术后并发症的发生情况,并计算其发生率.结果:实验组下颌阻生智齿拔除术后并发症的发生率低于对照组,经统计学分析处理两者有显著差异(P<0.05).结论:Gelatamp胶质银明胶海绵能有效预防下颌阻生智齿拔除术后并发症的发生,疗效肯定,值得临床推广应用.

  16. Analysis of PAHs in human serum using cloud point extraction and LC/MS

    Energy Technology Data Exchange (ETDEWEB)

    Sirimanne, S.R.; Ma, Li; McClure, C. [National Center for Health, Environmental Health and Lab. Sciences, Atlanta, GA (United States)

    1995-12-31

    In these laboratories, the authors assess human exposure to environmental toxicants by quantifying the levels of target toxicants in serum of exposed individuals. The levels of target analytes are often in trace levels and hence preconcentration and cleanup are prerequisites to the analysis of these trace toxicants. The authors have identified cloud point extraction as an impressive alternative to conventional solvent extraction due its beneficial properties and the greater extraction efficiencies. The authors report for the first time, extraction of PAHs from human serum using cloud point technology and analysis by HPLC.

  17. Lipid-lowering and antioxidant functions of bottle gourd (Lagenaria siceraria) extract in human dyslipidemia.

    Science.gov (United States)

    Katare, Charu; Saxena, Sonali; Agrawal, Supriya; Joseph, Anish Zacharia; Subramani, Senthil Kumar; Yadav, Dhananjay; Singh, Nita; Bisen, Prakash Singh; Prasad, G B K S

    2014-04-01

    The study validated the antidyslipidemic, antioxidant, and antihyperglycemic effects of Lagenaria siceraria fruit extract in human subjects with dyslipidemia along with subjects of normal health. A total of 200 mL of freshly prepared Lagenaria siceraria fruit extract was administered daily on empty stomach for 90 days. Significant reductions (P Lagenaria siceraria fruit extract exhibited significant antioxidant activity in dyslipidemic subjects as evident from elevations in SOD (P Lagenaria siceraria fruit extract serves as dietary adjunct in treatment of human dyslipidemia and cardiovascular disease.

  18. Non-compliance Appliances for Upper Molar Distalization: An Overview.

    Science.gov (United States)

    Noorollahian, Saeed; Alavi, Shiva; Shirban, Farinaz

    2015-01-01

    Tooth Size Arch-length Discrepancy (TSALD) is a common problem in orthodontics. Its clinical signs are tooth crowding, impaction and incisor proclination. The treatment options are dental arch expansion or tooth mass reduction (stripping or extraction). The "extraction versus non-extraction" controversy has been widely debated in the orthodontic literature. Distalization is a kind of arch expansion in anetro-posterior dimension. Several studies have evaluated both the therapeutic effectiveness and the side effects of the appliances for this method of space gaining. In some cases molar distalization is preferred, e.g., a patient with acceptable profile and skeletal pattern and half cusp Class II molar malocclusion or even less. In some cases molar distalization is the only way, e.g., the patient with previous upper premolar extraction and excessive overijet, or a skeletal Class III patient with previous upper premolar extraction needed upper anterior teeth retraction to create reverse overjet aspre surgical orthodontic decompensation. In this review article, we described non-compliance upper molar distalizing appliances.

  19. Studies of biological properties of Uncaria tomentosa extracts on human blood mononuclear cells.

    Science.gov (United States)

    Bors, Milena; Michałowicz, Jaromir; Pilarski, Radosław; Sicińska, Paulina; Gulewicz, Krzysztof; Bukowska, Bożena

    2012-08-01

    Uncaria tomentosa (Willd.) DC is a lignified climbing plant from South and Central America, which (under the name of "vilcacora" or "cat's claw") has become highly popular in many countries due to its proven immunostimmulatory and anti-inflammatory activities and also with respect to its anticancer and antioxidative effects. There are insufficient data on the mechanism of U. tomentosa action on normal blood mononuclear cells. The aim of the study was to analyze the impact of ethanol and aqueous extracts from bark and leaves of Uncaria tomentosa on the structure and function of human mononuclear cells and to find out whether the kind of extractant used modulates biological activity of the extracts studied. Plant material consisted of four different extracts: (1) ethanol extract from leaves, (2) aqueous extract from leaves, (3) ethanol extract from bark and (4) aqueous extract from bark. The effect of these extracts on protein damage as well as on free-radical formation in human peripheral blood mononuclear cells was analyzed. Moreover, changes in viability, size, and granularity as well as apoptotic alterations in human blood mononuclear cells exposed to U. tomentosa extracts were investigated. The oxidative changes were observed in mononuclear blood cells exposed to both ethanol and aqueous extracts obtained from bark and leaves. Moreover, in the cells studied the extracts from U. tomentosa induced apoptosis and a decrease in viability of mononuclear blood cells, with the exception of aqueous extract from leaves. Additionally, no statistically significant changes in the cell size were observed both for aqueous extracts from leaves and bark. Changes in the blood mononuclear cell granularity were observed at 250 μg/mL for all extracts examined. The strongest changes were observed for the ethanol extract of the bark, which increased cell granularity at 50 μg/mL and changed cell size at 100 μg/mL. The conducted research showed differences in biological activity

  20. Radiographic findings on 3rd molars removed in 20-year-old men.

    Science.gov (United States)

    Rajasuo, Ari; Peltola, Jaakko; Ventä, Irja; Murtomaa, Heikki

    2003-10-01

    In this study we assess radiographic findings characteristic of mandibular 3rd molars that had required either routine or surgical extraction. X-ray findings relating to acute pericoronitis were also examined. The material was collected by investigating patient records and rotational panoramic radiographs of 20-year-old Finnish male conscripts (n = 738) treated during military service because of 3rd-molar-related problems. The follicle around the crown of mandibular 3rd molars with acute pericoronitis was enlarged in 19% of cases and in 13% of chronic symptom-free pericoronitis cases (not statistically significant difference). Mandibular 3rd molars extracted surgically were more often mesially inclined than those extracted routinely (61% vs. 23%; P pericoronitis [around 27% vs. 39% of the teeth routinely extracted (OR 0.5, 95% CI 0.3-0.8)]. In 86% of cases the space between 2nd molar and ramus of the mandible was narrower than the 3rd molar extracted surgically, whereas this was 62% in routine extraction cases (P < 0.001). We conclude that there are some typical 3rd-molar findings in rotational panoramic radiographs that show a need for surgical extraction.

  1. Fate of third molar in line of mandibular angle fracture - Retrospective study

    Directory of Open Access Journals (Sweden)

    Preetha Balaji

    2015-01-01

    Conclusion: The present study fails to identify any concrete factors that would predict the failure of the retained third molar that were involved in the line of mandibular fractures. The proof presented here, especially with low complication rates indicate that all impacted third molar along the line of fracture be removed and unless necessary, the partially erupted teeth would also be extracted. In such a situation, the rate of infection and survival of the third molar would have been entirely different.

  2. Management of Unilaterally Impacted Permanent Mandibular First, Second and Third Molars

    Directory of Open Access Journals (Sweden)

    Rohit Mehrotra

    2014-01-01

    Full Text Available This case report describes the treatment of a teenage patient with simultaneous impaction of all three right mandibular permanent molars. The impacted first and second mandibular molars were surgically exposed and orthodontically erupted into good alignment and occlusion while the impacted third molar in the same quadrant was extracted. The unique clinical presentation, various treatment alternatives, the decision making process in finalizing the treatment plan and its clinical implementation are discussed.

  3. A curva de regressão da gonadotrofina coriônica humana é útil no diagnóstico precoce da neoplasia trofoblástica gestacional pós-molar? Are curves of human chorionic gonadotropin useful in the early diagnosis of post-molar trophoblastic neoplasia?

    Directory of Open Access Journals (Sweden)

    Lúcia Regina Marques Gomes Delmanto

    2007-10-01

    Full Text Available OBJETIVO: avaliar a utilidade da curva de regressão normal da gonadotrofina coriônica humana (hCG no diagnóstico precoce de neoplasia trofoblástica gestacional pós-molar (NTG. MÉTODOS: estudo longitudinal, incluindo 105 pacientes com mola hidatiforme completa (MHC acompanhadas no Centro de Doenças Trofoblásticas de Botucatu, entre 1998 e 2005. Os títulos da hCG sérica foram mensurados quinzenalmente em todas as pacientes. Curvas individuais de regressão da hCG das 105 pacientes foram estabelecidas. A comparação entre a curva de regressão normal estabelecida em nosso serviço com as curvas individuais da hCG foi usada no rastreamento e diagnóstico (platô/ascensão de NTG. O número de semanas pós-esvaziamento quando a hCG excedeu o limite normal foi comparado com o número semanas em que a hCG apresentou platô/ascensão. RESULTADOS: das 105 pacientes com MHC, 80 apresentaram remissão espontânea (RE e 25 desenvolveram NTG. Das 80 pacientes com RE, 7 (8,7% apresentaram, inicialmente, dosagem da hCG acima do normal, mas, no devido tempo, alcançaram a remissão. Todas as 25 pacientes com NTG apresentaram desvio da curva normal da hCG em 3,8±2,5 semanas e mostraram platô ou ascensão em 8,4±2,9 semanas (pPURPOSE: to evaluate the usefulness of the normal human chorionic gonadotropin (hCG regression curve in the early diagnosis of post-molar trophoblastic neoplasia (GTN. METHODS: a longitudinal study including 105 patients with complete hydatidiform mole (CHM followed up at the Botucatu Center of Trophoblastic Diseases from 1998 to 2005. Serial serum hCG titers were measured fortnightly in all patients. Individual curves of the 105 patients were built. Comparison between the normal regression curve established at our center with individual hCG curves was used to screen and diagnose (plateau/rise GTN. The number of weeks postevacuation when hCG levels exceeded the normal limits was compared with the number of weeks when h

  4. The expressions of NF-kb and TGFb-1 on odontoblast-like cells of human dental pulp injected with propolis extracts

    Directory of Open Access Journals (Sweden)

    Ira Widjiastuti

    2014-03-01

    Full Text Available Background: Propolis is known to have beneficial effects, namely anti- bacterial, anti-viral, anti-inflammatory, antioxidant, and immunomodulatory. Propolis extracts with anti-inflammatory properties are expected to be useful in treating inflamed pulp tissue with a diagnosis of reversible pulpitis. The inflammation of pulp tissue is caused by bacteria, namely Lactobacillus acidophilus. This research used odontoblast like cells derived from pulp tissue of human third molars. Odontoblast like cells exposed to Lactobacillus achidophilus were used as a model of proinflammatory cytokine signaling. This research examined the effects of propolis extracts on odontoblast like cells exposed to Lactobacillus acidophilus. Purpose: This research was aimed to determine the effectiveness of propolis extracts on the activities of odontoblast-like cells exposed to Lactobacillus acidophillus by measuring the expressions of NFkb and TGF- b1. Methods: First, pulp odontoblast cultures were derived from human dental pulp tissues of impacted third molars removed by using digestion method. Next, odontoblast-like cells exposed to inactive Lactobacillus acidophilus bacteria were given propolis extract. Finally, the activities of odontoblast-like cells were monitored by measuring the expressions of NF-kb and TGFb-1 with immunocytochemistry technique. Results: A decline NF-kb expression and on increase of TGFb-1 expression on odontoblast like cells exposed to inactive Lactobacillus acidophilus. Conclusion: Propolis extracts inhibit the expression of NF-kb, and increase the expression of TGF-b1 in pulp odontoblast-like cells exposed to inactive Lactobacillus acidophillus.Latar belakang: Propolis dilaporkan mempunyai efek menguntungkan yaitu bersifat anti bakteri, anti virus, anti inflamasi, anti oksidan, dan imunomodulator. Ekstrak propolis dengan sifat anti inflamasi diharapkan bermanfaat untuk mengobati jaringan pulpa yang mengalami inflamasi dengan diagnosis pulpitis

  5. Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

    DEFF Research Database (Denmark)

    Vårdal, Linda; Gjelstad, Astrid; Huang, Chuixiu

    2017-01-01

    AIM: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to invest...

  6. Parallel artificial liquid membrane extraction of acidic drugs from human plasma

    DEFF Research Database (Denmark)

    Roldan-Pijuan, Mercedes; Pedersen-Bjergaard, Stig; Gjelstad, Astrid

    2015-01-01

    The new sample preparation concept “Parallel artificial liquid membrane extraction (PALME)” was evaluated for extraction of the acidic drugs ketoprofen, fenoprofen, diclofenac, flurbiprofen, ibuprofen, and gemfibrozil from human plasma samples. Plasma samples (250 μL) were loaded into individual...

  7. Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

    Directory of Open Access Journals (Sweden)

    Nurfariza Ahmad Roslen

    2014-07-01

    Conclusions: The extracts from leaves and flowers of M. malabathricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50 at 7.14 μg/mL while the extracts from stems showed less growth inhibition activity.

  8. Strategies for Prospecting and Extracting Water on Mars for Long-Term Human Exploration

    Science.gov (United States)

    Rolley, R. J.; Saikia, S. J.

    2017-02-01

    We aim to develop a specific set of criteria to classify water reserves on Mars, and to design water prospecting and extraction systems for various human landing sites using a requirements-driven framework.

  9. Extraction of human genomic DNA from whole blood using a magnetic microsphere method.

    Science.gov (United States)

    Gong, Rui; Li, Shengying

    2014-01-01

    With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time-consuming, and phenol and chloroform are toxic reagents that endanger health. Therefore, it is necessary to find a more convenient and efficient method for obtaining human genomic DNA. In this study, we developed urea-formaldehyde resin magnetic microspheres and magnetic silica microspheres for extraction of human genomic DNA. First, a magnetic microsphere suspension was prepared and used to extract genomic DNA from fresh whole blood, frozen blood, dried blood, and trace blood. Second, DNA content and purity were measured by agarose electrophoresis and ultraviolet spectrophotometry. The human genomic DNA extracted from whole blood was then subjected to polymerase chain reaction analysis to further confirm its quality. The results of this study lay a good foundation for future research and development of a high-throughput and rapid extraction method for extracting genomic DNA from various types of blood samples.

  10. Erupted complex odontoma delayed eruption of permanent molar.

    Science.gov (United States)

    Ohtawa, Yumi; Ichinohe, Saori; Kimura, Eri; Hashimoto, Sadamitsu

    2013-01-01

    Odontomas, benign tumors that develop in the jaw, rarely erupt into the oral cavity. We report an erupted odontoma which delayed eruption of the first molar. The patient was a 10-year-old Japanese girl who came to our hospital due to delayed eruption of the right maxillary first molar. All the deciduous teeth had been shed. The second premolar on the right side had erupted, but not the first molar. Slight inflammation of the alveolar mucosa around the first molar had exposed a tooth-like, hard tissue. Panoramic radiography revealed a radiopaque mass indicating a lesion approximately 1 cm in diameter. The border of the image was clear, and part of the mass was situated close to the occlusal surface of the first molar. The root of the maxillary right first molar was only half-developed. A clinical diagnosis of odontoma was made. The odontoma was subsequently extracted, allowing the crown of the first molar to erupt almost 5 months later. The dental germ of the permanent tooth had been displaced by the odontoma. However, after the odontoma had been extracted, the permanent tooth was still able to erupt spontaneously, as eruptive force still remained. When the eruption of a tooth is significantly delayed, we believe that it is necessary to examine the area radiographically. If there is any radiographic evidence of a physical obstruction that might delay eruption, that obstruction should be removed before any problems can arise. Regular dental checkups at schools might improve our ability to detect evidence of delayed eruption earlier.

  11. Tissue engineered bone scaffold material in restoration of alveolar socket after extraction of lower impacted third molar%骨组织工程支架材料充填下颌阻生智牙拔牙窝的临床效果

    Institute of Scientific and Technical Information of China (English)

    陈志方

    2011-01-01

    PURPOSE: To investigate the effects of tissue engineered bone scaffold material in the restoration of alveolar socket after extraction of lower impacted third molar. METHODS: Thirteen patients were immediately implanted with Bio-oss or PerioGlas~ in the alveolar cavity after impacted mandibular third molar extraction. Clinical observation and Xray were taken I to 12 weeks after operation. Paired t test was used for statistical analysis by SPSS10.0 software package.RESULTS: Thirteen patients did not experience postoperative complications. The distal alveolar height of the second molar and the gingival attachment did decrease significantly 1 to 12 weeks after operation (P<0.05). CONCLUSION:Tissue engineered bone scaffold material is helpful in the restoration of alveolar socket after impacted third molar extraction.%目的:探讨下颌阻生智牙拔除后,用骨组织工程支架材料充填拔牙窝,修复第二磨牙远中牙槽骨高度的临床效果.方法:13例患者在阻生智牙拔除后,即刻植入组织工程骨微粒(小牛无机松质骨Bio-oss、倍骼生PerioGlasf?)于牙槽窝内,术后定期随访,并从临床和X线影像检查比较术后1、12周时,第二磨牙远中牙龈附着水平和牙槽嵴高度的变化.采用SPSS10.0软件包对数据进行配对样本t检验.结果:所有患者术后未出现并发症,术后12周时,第二磨牙远中牙槽嵴高度显著降低(P<0.05).结论:下颌阻生智牙拔除术后即刻植入组织工程骨支架材料,有利于保持第二磨牙远中牙槽嵴的高度.

  12. Cytotoxic activities of Coriolus versicolor (Yunzhi) extracts on human ...

    African Journals Online (AJOL)

    four human liver cancer (7703, HepG2, 7721, PLC) and four human breast cancer (Bcap37, ZR75-30, MCF-7, T-47D) cell lines using a MTT cytotoxicity assay. The results showed that the CVP ... Article Metrics. Metrics Loading ... Metrics ...

  13. ASTHMATIC HUMAN SERUM IGE-REACTIVITY WITH MOLD EXTRACTS

    Science.gov (United States)

    Although molds have demonstrated the ability to induce allergic asthma-like responses in mouse models, their role in human disease is unclear. This study was undertaken to provide insight into the prevalence of human IgE-reactivity and identify the target mold protein(s). The st...

  14. Complications of third molar surgery.

    Science.gov (United States)

    Bouloux, Gary F; Steed, Martin B; Perciaccante, Vincent J

    2007-02-01

    This article addresses the incidence of specific complications and, where possible, offers a preventive or management strategy. Injuries of the inferior alveolar and lingual nerves are significant issues that are discussed separately in this text. Surgical removal of third molars is often associated with postoperative pain, swelling, and trismus. Factors thought to influence the incidence of complications after third molar removal include age, gender, medical history, oral contraceptives, presence of pericoronitis, poor oral hygiene, smoking, type of impaction, relationship of third molar to the inferior alveolar nerve, surgical time, surgical technique, surgeon experience, use of perioperative antibiotics, use of topical antiseptics, use of intra-socket medications, and anesthetic technique. Complications that are discussed further include alveolar osteitis, postoperative infection, hemorrhage, oro-antral communication, damage to adjacent teeth, displaced teeth, and fractures.

  15. Human colon cancer HT-29 cell death responses to doxorubicin and Morus Alba leaves flavonoid extract.

    Science.gov (United States)

    Fallah, S; Karimi, A; Panahi, G; Gerayesh Nejad, S; Fadaei, R; Seifi, M

    2016-03-31

    The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. MFE and doxorubicin exert a cytotoxic effect on human colon cancer HT-29 cell line by probably promoting or induction of apoptosis.

  16. Comparative Analysis of High Speed Turbine Drill and Traditional Chisel Method of Extracting Impacted Mandibular Third Molar in Clinic%高速涡轮钻法与传统骨凿法拔除下颌阻生齿临床比较分析

    Institute of Scientific and Technical Information of China (English)

    赵东强; 韩磊; 刘宾

    2015-01-01

    Objective Comparative analysis to study on the clinical application of high speed turbine to crown extraction method and traditional chisel method of extracting impacted mandibular third molar. Methods In our hospital for 120 cases of mandibular impacted tooth requirements of patients, they are randomly divided into two groups:group of experiments to crown in 60 cases using high speed turbine after extraction of mandibular impacted teeth, 60 cases in the control group using the traditional method of extracting impacted mandibular third molar chisel, by recording the operation time, postoperative mouth by degree, degree of pain and incidence of dry socket. Results In the observation group, operation time, postoperative pain, mouth by the degree and incidence of dry socket is significantly lower than the control group, the dif erence was statistical y significant ( <0.05). Conclusion Using high speed turbine dril ing method for extraction of mandibular impacted third molar is low, operation time and trauma decreased significantly, and reduce the incidence of postoperative complications, So it is worth to be promoted and popularized in clinical practice.%目的:探讨高速涡轮机去冠拔牙法与传统骨凿法拔除下颌阻生齿的临床应用效果的比较分析。方法选取我:因下颌阻生齿要求拔牙的患者120例,随机分为两组:实验组60例应用高速涡轮机去冠后拔除下颌阻生齿,对照组60例应用传统骨凿法拔除下颌阻生齿,通过记录手术时间、术后张口受程度、疼痛程度及干槽症发病情况,进行两组临床对比观察。结果观察组手术时间、术后张口受程度、疼痛程度及干槽症发病情况为显著低于对照组,差异有统计学意义(<0.05)。结论使用高速涡轮钻法拔除下颌低阻生智齿,手术时间及创伤明显减少,降低术后并发症的发生,具有临床使用及推广价值。

  17. Unusual anatomical detection of a third molar in the infratemporal fossa.

    Science.gov (United States)

    Corega, C; Vaida, L; Festila, D; Bertossi, D

    2013-01-01

    Third molar presence in the infratemporal fossa is a rare event and it has been reported previously only two times in the literature, except for the cases which arise from complications occurring during the extraction of the impacted upper third molar. Due to the presence of important vessel bundles and nerves in this area, third molar removal requires a correct surgical management in order to avoid many possible serious side effects. We report an unusual case of upper third molar detected in the infratemporal fossa, which has been thoroughly investigated radiologically and removed through a safe surgical approach.

  18. Development and validation of an extraction method for the analysis of perfluoroalkyl substances in human hair.

    Science.gov (United States)

    Kim, Da-Hye; Oh, Jeong-Eun

    2017-02-16

    Human hair has many advantages as a non-invasive sample; however, analytical methods for detecting perfluoroalkyl substances (PFASs) in human hair are still in the development stage. Therefore, the aim of this study was to develop and validate a method for monitoring 11 PFASs in human hair. Solid-phase extraction (SPE), ion-pairing extraction (IPE), a combined method (SPE+IPE) and solvent extraction with ENVI-carb clean-up were compared to develop an optimal extraction method using two types of hair sample (powder and piece forms). Analysis of PFASs was performed using liquid chromatography and tandem mass spectrometry. Among the four different extraction procedures, the SPE method using powdered hair showed the best extraction efficiency and recoveries ranged from 85.8 to 102%. The method detection limits for the SPE method were 0.114-0.796 ng/g and good precision (below 10%) and accuracy (66.4-110%) were obtained. In light of these results, SPE is considered the optimal method for PFAS extraction from hair. It was also successfully used to detect PFASs in human hair samples.

  19. Hypomineralized Second Primary Molars as Predictor of Molar Incisor Hypomineralization.

    Science.gov (United States)

    Negre-Barber, A; Montiel-Company, J M; Boronat-Catalá, M; Catalá-Pizarro, M; Almerich-Silla, J M

    2016-08-25

    Molar incisor hypomineralization (MIH) is a developmental defect of dental enamel that shares features with hypomineralized second primary molars (HSPM). Prior to permanent tooth eruption, second primary molars could have predictive value for permanent molar and incisor hypomineralization. To assess this possible relationship, a cross-sectional study was conducted in a sample of 414 children aged 8 and 9 years from the INMA cohort in Valencia (Spain). A calibrated examiner (linear-weighted Kappa 0.83) performed the intraoral examinations at the University of Valencia between November 2013 and 2014, applying the diagnostic criteria for MIH and HSPM adopted by the European Academy of Paediatric Dentistry. 100 children (24.2%) presented MIH and 60 (14.5%) presented HSPM. Co-occurrence of the two defects was observed in 11.1% of the children examined. The positive predictive value was 76.7% (63.9-86.6) and the negative predictive value 84.7% (80.6-88.3). The positive likelihood ratio (S/1-E) was 10.3 (5.9-17.9) and the negative likelihood ratio (1-S/E) 0.57 (0.47-0.68). The odds ratio was 18.2 (9.39-35.48). It was concluded that while the presence of HSPM can be considered a predictor of MIH, indicating the need for monitoring and control, the absence of this defect in primary dentition does not rule out the appearance of MIH.

  20. The Role of Mandibular Third Molars on Lower Anterior Teeth Crowding and Relapse after Orthodontic Treatment: A Systematic Review

    Directory of Open Access Journals (Sweden)

    Khalid H. Zawawi

    2014-01-01

    Full Text Available Aims. To evaluate the role of third molars in the development of crowding or relapse after orthodontic treatment in the anterior segment of the dental arch. Methods. PubMed search of the literature was performed selecting all the articles relevant to the topic and limiting the studies to controlled trials on humans and written in English language. Systematic review was conducted according to the PRISMA (preferred reporting items for systematic reviews and meta-analyses statement. Results. A total of 12 clinical studies were included in the review. A high risk of bias was found in most of the articles, either because the relative items assessed were inadequate or because they were unclearly described. The third molars were not correlated with more severe anterior tooth crowding in most of the studies. However, four of them described a different outcome. Conclusion. Definitive conclusions on the role of the third molars in the development of anterior tooth crowding cannot be drawn. A high risk of bias was found in most of the trials, and the outcomes were not consistent. However, most of the studies do not support a cause-and-effect relationship; therefore, third molar extraction to prevent anterior tooth crowding or postorthodontic relapse is not justified.

  1. A preliminary study of effects of immediate bone grafting at mandibular first molar fresh extraction socketon maintaining alveolar bone height in adult orthodontic patients%成人正畸患者减数下颌第一恒磨牙即刻植骨对于牙槽嵴保持作用的初步研究

    Institute of Scientific and Technical Information of China (English)

    陈启兴; 周治波; 周彦恒

    2014-01-01

    Objective To evaluate the effect of immediate bone graftingat fresh mandibular first molar extraction socket on maintain alveolar bone height during orthodontic treatment.Methods 32 adult orthodontic patients withmandibular first molars extraction treatment plan were selected.Thetotal of 40 molar extraction sites were divided into graft group and non-graft group randomly.All patients received orthodontic treatment 2weeks after extraction (with or without bone graft).Cone beam CT were taken before (T0)and one year after extraction(T1).Compare the changes of alveolar height and width of two groups by Invivo Dental 5.0 software.Results One year after orthodontic treatment,extraction spacesingraft group and non-graft group decreased by an average of 2.85 mm and 3.50 mm respectively.In graft group,the alveolar width at 3 mm and 6mrn level from alveolarcrest decreased by 0.71 mm and 0.39 mm.In non-graft group,the alveolar width at 3 mm,6 mm and 9 mm level from alveolarcrest decreased by 4.1 mm,2.4 mm and 1.1 mm.Paired t-test showed significant changes (P<0.01).The alveolar height in non-graft group reduced by 1.72 rnm,the difference is significant (P<0.01),while graft group has no significant difference.Conclusions For orthodontic patients with mandibular first molar extraction plan,imeediatebone grafting after extraction can delaythe resorption of the alveolar bone crest,facilitating long-distance space closure.%目的 评价减数下颌第一恒磨牙后即刻植骨对正畸治疗中牙槽嵴的保持效果.方法 选取32名需要减数下颌第一恒磨牙的成年正畸患者,共40个牙位.随机分成拔牙后即刻植骨与非植骨两组.拔牙(植骨)后,患者均进行常规正畸治疗.拍摄拔牙前(T0)、拔牙后一年(T1)的锥体束CT,采用Invivo Dental 5.0软件进行三维测量,比较两组T0~T1牙槽嵴高度、宽度的变化.结果 植骨组在拔牙后一年,距离牙槽嵴顶3mm、6mm处牙槽嵴宽度分别减小了0.71 mm,0.39 mm

  2. Inhibitory effects of pomegranate extracts on recombinant human maltase-glucoamylase.

    Science.gov (United States)

    Kawakami, Kayoko; Li, Peng; Uraji, Misugi; Hatanaka, Tadashi; Ito, Hideyuki

    2014-09-01

    α-Glucosidase inhibitors are currently used in the treatment of type 2 diabetes. In this study, we investigated the inhibitory activities of aril and pericarp extracts from pomegranates obtained various regions against recombinant human maltase-glucoamylase (MGAM). The inhibitory activities of the aril extracts tended to be stronger than those of the pericarp extracts. The Iranian aril extract was the most effective inhibitor. We investigated the polyphenol content of the pomegranate extracts using the Folin-Ciocalteu method. Among the aril extracts, the Iranian aril extract showed the highest polyphenol content. We further evaluated inhibitory activity against α-glucosidase from the rat small intestine. Pomegranate extract used in this study showed slightly different inhibitory activities according to α-glucosidase origin. Iranian aril extract was the most effective inhibitor of α-glucosidases, especially recombinant human MGAM. Bioassay-guided fractionation of the pomegranate arils led to identification of punicalagin and oenothein B as potent inhibitors of α-glucosidase. Oenothein B showed inhibitory activity with a half-maximal inhibitory concentration (IC(50)) value of 174 μM. Its potency was comparable to that of the α-glucosidase inhibitor acarbose with an IC(50) value of 170 μM. Dixon plot kinetic analysis of oenothein B showed a noncompetitive inhibition with a K(i) value of 102 μM. These results suggest that pomegranate arils would be useful for suppressing postprandial hyperglycemia.

  3. Apoptosis induction of Persicae Semen extract in human promyelocytic leukemia (HL-60) cells.

    Science.gov (United States)

    Kwon, Hee-Young; Hong, Seon-Pyo; Hahn, Dong-Hoon; Kim, Jeong Hee

    2003-02-01

    The major ingredient of Persicae Semen is a cynogenic compound, amygdalin (D-mandelonitrile-beta-gentiobioside). Controversial results on the anticancer activity of amygdalin were reported due to its conversion to its inactive isomer, neoamygdalin. In order to inhibit the epimerization of amygdalin, we used newly developed simple acid boiling method in preparation of Persicae Semen extract. HPLC analysis revealed most of amygdalin in Persicae Semen extract was active D-form. Persicae Semen extract was used to analyze its effect on cell proliferation and induction of apoptosis in human promyelocytic leukemia (HL-60) cells. Persicae Semen extract was cytotoxic to HL-60 cells with IC50 of 6.4 mg/mL in the presence of 250 nM of beta-glucosidase. The antiproliferative effects of Persicae Semen extract appear to be attributable to its induction of apoptotic cell death, as Persicae Semen extract induced nuclear morphology changes and internucleosomal DNA fragmentation.

  4. Human Cytosolic Extracts Stabilize the HIV-1 Core

    Science.gov (United States)

    Fricke, Thomas; Brandariz-Nuñez, Alberto; Wang, Xiaozhao; Smith, Amos B.

    2013-01-01

    The stability of the HIV-1 core in the cytoplasm is crucial for productive HIV-1 infection. Mutations that stabilize or destabilize the core showed defects on HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure the stability of in vitro-assembled HIV-1 CA-NC complexes. The assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core. Interestingly, stabilization of in vitro-assembled HIV-1 CA-NC complexes is not due solely to macromolecular crowding, suggesting the presence of specific cellular factors that stabilize the HIV-1 core. By using our novel assay, we measured the abilities of different drugs, such as PF74, CAP-1, IXN-053, cyclosporine, Bi2 (also known as BI-2), and the peptide CAI, to modulate the stability of in vitro-assembled HIV-1 CA-NC complexes. Interestingly, we found that PF74 and Bi2 strongly stabilized HIV-1 CA-NC complexes. On the other hand, the peptide CAI destabilized HIV-1 CA-NC complexes. We also found that purified cyclophilin A destabilizes in vitro-assembled HIV-1 CA-NC complexes in the presence of cellular extracts in a cyclosporine-sensitive manner. In agreement with previous observations using the fate-of-the-capsid assay, we also demonstrated the ability of recombinant CPSF6 to stabilize HIV-1 CA-NC complexes. Overall, our findings suggested that cellular extracts specifically stabilize the HIV-1 core. We believe that our assay can be a powerful tool to assess HIV-1 core stability in vitro. PMID:23885082

  5. Structure-activity analysis of histatin, a potent wound healing peptide from human saliva: cyclization of histatin potentiates molar activity 1000-fold

    NARCIS (Netherlands)

    Oudhoff, M.J.; Kroeze, K.L.; Nazmi, K.; van den Keijbus, P.A.M.; van 't Hof, W.; Fernandez-Borja, M.; Hordijk, P.L.; Gibbs, S.; Bolscher, J.G.M.; Veerman, E.C.I.

    2009-01-01

    Wounds in the mouth heal faster and with less scarification and inflammation than those in the skin. Saliva is thought to be essential for the superior oral wound healing, but the involved mechanism is still unclear. We have previously discovered that a human-specific peptide, histatin, might be imp

  6. Etiology, Classification and Management of Ectopic Eruption of Permanent First Molars.

    Science.gov (United States)

    Hsiao, Chu-Chun; Boynton, James R

    2016-01-01

    Ectopic eruption of a permanent tooth involves abnormal resorption of a portion or all of the adjacent primary tooth. Among the most commonly ectopically erupted teeth are the permanent first molars. Ectopically erupting molars may require intervention to allow for full eruption, or they may spontaneously self-correct and erupt into occlusion. Decisions regarding the necessity of intervention, its ideal timing, and intervention type are multifactorial. Treatment options for the ectopically erupting permanent first molar include the elastomeric separator, brass wire, pre-fabricated clip separator, custom made appliances (Humphrey appliance, Halterman appliance), or extraction of the primary molar. Early intervention when indicated can ensure proper full eruption of the permanent first molar and prevent mesial angulation, arch perimeter loss, tooth impaction and ankylosis. Two cases are described that manage ectopic eruption of the permanent first molar.

  7. Mechanism of cytotoxicity by Psoralea corylifolia extract in human breast carcinoma cells.

    Science.gov (United States)

    Rajan, Vasumathy; Tripathi, Jyoti; Variyar, Prasad; Pandey, Badri Narain

    2014-01-01

    Psoralea corylifolia has been widely used in herbal medicine, and a few studies show its anticancer activity. However, the detailed mechanism of the anticancer activity of P. corylifolia seed extract (PC extract) was not studied. This study evaluates the anticancer activity and underlying mechanism of PC extract in a human breast cancer cell line (MCF7). PC extract caused a concentration-dependent decrease in the proliferation of MCF7 cells and an increase in apoptotic death as measured by annexin-V-FITC and TUNEL assays. Increased cleavage of poly(ADP-ribose) polymerase in cells treated with PC extract further confirmed the apoptotic mode of cell death. There was a decrease (~2-fold) of mitochondrial membrane potential in cells treated with PC extract. In cells treated with PC extract, an increase in intracellular reactive oxygen species (ROS) and a decrease in mitochondrial ROS was observed. A significant decrease in ATP (~1.8-fold) was observed in extract-treated cells. Moreover, MCF7 cells treated with extract showed cleavage of caspase-9 and caspase-7, upregulation of Bax, release of cytochrome-c, and loss of mitochondrial integrity. Taken together, these results suggest the involvement of the mitochondrial pathway in PC extract-induced apoptosis in MCF7 cells.

  8. EXTRACT

    DEFF Research Database (Denmark)

    Pafilis, Evangelos; Buttigieg, Pier Luigi; Ferrell, Barbra

    2016-01-01

    The microbial and molecular ecology research communities have made substantial progress on developing standards for annotating samples with environment metadata. However, sample manual annotation is a highly labor intensive process and requires familiarity with the terminologies used. We have the...... and text-mining-assisted curation revealed that EXTRACT speeds up annotation by 15-25% and helps curators to detect terms that would otherwise have been missed.Database URL: https://extract.hcmr.gr/....

  9. Non‑third molar related pericoronitis in a sub‑urban Nigeria ...

    African Journals Online (AJOL)

    2013-02-28

    Feb 28, 2013 ... that extraction of a third molar as a result of pericoronitis may precipitate upper ... were treated with antibiotics (amoxicillin), analgesic. (paracetamol) .... low prevalence of these micro‑organisms in the oral flora of children may ...

  10. Molar incisor hypomineralization, prevalence, and etiology.

    Science.gov (United States)

    Allazzam, Sulaiman Mohammed; Alaki, Sumer Madani; El Meligy, Omar Abdel Sadek

    2014-01-01

    Aim. To evaluate the prevalence and possible etiological factors associated with molar incisor hypomineralization (MIH) among a group of children in Jeddah, Saudi Arabia. Methods. A group of 8-12-year-old children were recruited (n = 267) from the Pediatric Dental Clinics at the Faculty of Dentistry, King Abdulaziz University. Children had at least one first permanent molar (FPM), erupted or partially erupted. Demographic information, children's medical history, and pregnancy-related data were obtained. The crowns of the FPM and permanent incisors were examined for demarcated opacities, posteruptive breakdown (PEB), atypical restorations, and extracted FPMs. Children were considered to have MIH if one or more FPM with or without involvement of incisors met the diagnostic criteria. Results. MIH showed a prevalence of 8.6%. Demarcated opacities were the most common form. Maxillary central incisors were more affected than mandibular (P = 0.01). The condition was more prevalent in children with history of illnesses during the first four years of life including tonsillitis (P = 0.001), adenoiditis (P = 0.001), asthma (P = 0.001), fever (P = 0.014), and antibiotics intake (P = 0.001). Conclusions. The prevalence of MIH is significantly associated with childhood illnesses during the first four years of life including asthma, adenoid infections, tonsillitis, fever, and antibiotics intake.

  11. Molar Incisor Hypomineralization, Prevalence, and Etiology

    Directory of Open Access Journals (Sweden)

    Sulaiman Mohammed Allazzam

    2014-01-01

    Full Text Available Aim. To evaluate the prevalence and possible etiological factors associated with molar incisor hypomineralization (MIH among a group of children in Jeddah, Saudi Arabia. Methods. A group of 8-12-year-old children were recruited (n=267  from the Pediatric Dental Clinics at the Faculty of Dentistry, King Abdulaziz University. Children had at least one first permanent molar (FPM, erupted or partially erupted. Demographic information, children’s medical history, and pregnancy-related data were obtained. The crowns of the FPM and permanent incisors were examined for demarcated opacities, posteruptive breakdown (PEB, atypical restorations, and extracted FPMs. Children were considered to have MIH if one or more FPM with or without involvement of incisors met the diagnostic criteria. Results. MIH showed a prevalence of 8.6%. Demarcated opacities were the most common form. Maxillary central incisors were more affected than mandibular (P=0.01. The condition was more prevalent in children with history of illnesses during the first four years of life including tonsillitis (P=0.001, adenoiditis (P=0.001, asthma (P=0.001, fever (P=0.014, and antibiotics intake (P=0.001. Conclusions. The prevalence of MIH is significantly associated with childhood illnesses during the first four years of life including asthma, adenoid infections, tonsillitis, fever, and antibiotics intake.

  12. Onset of molar incisor hypomineralization (MIH).

    Science.gov (United States)

    Fagrell, Tobias G; Salmon, Phil; Melin, Lisa; Norén, Jörgen G

    2013-01-01

    The etiological factors and timing of the onset of molar incisor hypomineralization (MIH) are still not clear. The aim of this study was to examine ground radial and sagittal sections from teeth diagnosed with MIH using light microscopy, polarized light microscopy and X-ray micro-computed tomography (XMCT) and to estimate the onset and timing of the MIH and to relate the hypomineralized enamel to the incremental lines. Thirteen extracted permanent first molars diagnosed MIH, were analyzed with light microscopy and XMCT. The hypomineralized areas were mainly located in the mesio-buccal cusps, starting at the enamel-dentin-junction and continuing towards the enamel surface. In a relative gray scale analysis the values decreased from the EDJ towards the enamel surface. The findings indicate that the ameloblasts in the hypomineralized enamel are capable of forming an enamel of normal thickness, but with a substantial reduction of their capacity for maturation of enamel. Chronologically, it is estimated that the timing of the disturbance is at a period during the first 6-7 months of age.

  13. Agenesis of all third molars in two half siblings

    Directory of Open Access Journals (Sweden)

    Jamie A Kaufer

    2015-04-01

    Full Text Available Tooth agenesis is one of the most common dental anomalies and is influenced by factors including patient genetics. Although there are several specific genes associated with certain patterns of agenesis, there does not seem to be a genetic pattern identified to date that is associated with isolated complete third molar agenesis. This report presents two half-siblings who both express complete agenesis of third molars despite the fact that their shared parent does not express the same phenotype. The case discussion focuses on addressing the potential genetic possibilities including autosomal dominant inheritance and variable expressivity of a mutation.  There remains an uncertainty to the exact gene involved. Potential options include WNT10A and PAX9. The affected individuals are not needing extractions or experiencing complications due to the presence of third molars.  However, the concern remains that there is the potential for more severe expression of the mutation in future generations.

  14. Use of sevoflurane inhalation sedation for outpatient third molar surgery.

    Science.gov (United States)

    Ganzberg, S; Weaver, J; Beck, F M; McCaffrey, G

    1999-01-01

    This study attempted to determine if sevoflurane in oxygen inhaled via a nasal hood as a sole sedative agent would provide an appropriate level of deep sedation for outpatient third molar surgery. Twenty-four patients scheduled for third molar removal were randomly assigned to receive either nasal hood inhalation sevoflurane or an intravenous deep sedation using midazolam and fentanyl followed by a propofol infusion. In addition to measuring patient, surgeon, and dentist anesthesiologist subjective satisfaction with the technique, physiological parameters, amnesia, and psychomotor recovery were also assessed. No statistically significant difference was found between the sevoflurane and midazolam-fentanyl-propofol sedative groups in physiological parameters, degree of amnesia, reported quality of sedation, or patient willingness to again undergo a similar deep sedation. A trend toward earlier recovery in the sevoflurane group was identified. Sevoflurane can be successfully employed as a deep sedative rather than a general anesthetic for extraction of third molars in healthy subjects.

  15. First permanent molar root development arrest associated with compound odontoma.

    Science.gov (United States)

    Gunda, Sachin A; Patil, Anil; Varekar, Aniruddha

    2013-07-04

    Trauma or infection to the primary tooth may have deleterious effects on the underlying developing tooth buds. Anatomically the root apices of primary teeth are in close proximity to the developing permanent tooth buds; hence spread of infection originating from pulp necrosis of primary tooth may not only affect the underlying tooth bud but may also affect the adjacent tooth buds. The extent of malformation depends on the developmental stage of tooth or the age of patient. Presented here is a rare case of complete arrest of maxillary first permanent molar root growth due to spread of periapical infection originating from second primary molar leading to failure of its eruption and finally extraction. Histopathlogical analysis revealed compound odontoma associated with maxillary first permanent molar.

  16. Comparison of micro-computerized tomography and cone-beam computerized tomography in the detection of accessory canals in primary molars

    Energy Technology Data Exchange (ETDEWEB)

    Acar, Buket; Kamburoglu, Kivanc [Dept. of Dentomaxillofacial Radiology, Faculty of Dentistry, Ankara University, Ankara (Turkmenistan); Tatar, Ilkan [Dept. of Anatomy, Faculty of Medicine, Hacettepe University, Ankara (Turkmenistan); Arikan, Volkan [Dept. of Anatomy, Faculty of Medicine, Hacettepe University, Ankara (Turkmenistan); Celik, Hakan Hamid [Dept. of Pediatric Dentistry, Faculty of Dentistry, Kirikkale Unversity, Ankara (Turkmenistan); Yuksel, Selcen [Dept. of Biostatistics, Faculty of Medicine, Yildirim Beyazit University, Ankara (Turkmenistan); Ozen, Tuncer [Dept. of Dentomaxillofacial Radiology, Gulhane Military Hospital, Dental Clinics, Ankara (Turkmenistan)

    2015-12-15

    This study was performed to compare the accuracy of micro-computed tomography (CT) and cone-beam computed tomography (CBCT) in detecting accessory canals in primary molars. Forty-one extracted human primary first and second molars were embedded in wax blocks and scanned using micro-CT and CBCT. After the images were taken, the samples were processed using a clearing technique and examined under a stereomicroscope in order to establish the gold standard for this study. The specimens were classified into three groups: maxillary molars, mandibular molars with three canals, and mandibular molars with four canals. Differences between the gold standard and the observations made using the imaging methods were calculated using Spearman's rho correlation coefficient test. The presence of accessory canals in micro-CT images of maxillary and mandibular root canals showed a statistically significant correlation with the stereomicroscopic images used as a gold standard. No statistically significant correlation was found between the CBCT findings and the stereomicroscopic images.Although micro-CT is not suitable for clinical use, it provides more detailed information about minor anatomical structures. However, CBCT is convenient for clinical use but may not be capable of adequately analyzing the internal anatomy of primary teeth.

  17. Study of the Major Components of the PluriNet Network Expression in Dental Pulp Stem Cells Isolated from Human Third Molar Teeth

    Directory of Open Access Journals (Sweden)

    Behnam Ebrahimi

    2010-01-01

    Full Text Available Objective: The objective of this study was to isolate and culture human dental pulp stemcells to study important stem cell markers in them.Materials and Methods: Dental stem cells were isolated from human pulp and culturedin alpha-modified eagle’s medium (α-MEM supplemented with 20% fetal bovine serum(FBS in a 37°C incubator with 5% CO2 and photographed under inverted microscope.The expressions of the important stem cell markers were analyzed by reverse transcriptionpolymerase chain reaction (RT-PCR and agarose gel electrophoresis in the cells atdifferent passages.Results: Cells isolated from dental pulp showed a high rate of proliferation and werecultured to more than 15 passages in vitro. The study of gene expression by RT-PCRshowed that these cells expressed nucleostemin, cyclin D1, Oct-4 and nanog (major componentsof the PluriNet in different passages as well as under serum-free conditions.Conclusion: Cells isolated from dental pulp are genuine pluripotent stem cells with highpotential for self-renewal. The expression of the stem cell markers in human dental pulpstem cells indicate that they have a great potential for cell therapy and regenerative medicine,even they were isolated from adult teeth.

  18. Subcutaneous emphysema during third molar surgery: a case report.

    Science.gov (United States)

    Romeo, Umberto; Galanakis, Alexandros; Lerario, Francesco; Daniele, Gabriele Maria; Tenore, Gianluca; Palaia, Gaspare

    2011-01-01

    Extraction of third molars is the most common surgical procedure performed in oral surgery on a daily basis and, despite surgical skills and expertise, complications may occur. Complications observed during or after third molar removal may include pain, swelling, bleeding, infection, sinus perforation and nerve damage. Fortunately, with a proper management and a good surgical technique, the incidence of such events is low. Subcutaneous emphysema associated with dental extraction occurs when the air from the high-speed dental handpiece is forced into the soft tissue through the reflected flap and invades the adjacent tissues, leading to swelling, crepitus on palpation and occasionally spreading through the tissue spaces of the fascial planes. Although rare, iatrogenic subcutaneous emphysema can have serious and potentially life-threatening consequences. Care should be taken when using air-driven handpieces. The access of air into the facial tissues is not limited to tooth extractions, but may also occur through other portals of entrance, such as endodontically treated teeth, periodontium and lacerations of intraoral soft tissues. When subcutaneous emphysema occurs, it must be quickly diagnosed and properly managed to reduce the risk of further complications. This report presents a case of subcutaneous emphysema occurred during extraction of a mandibular third molar extraction with the use of an air turbine handpiece. Case management is described and issues relative to the diagnosis and prevention of this surgical complication are discussed.

  19. DNA extraction from fresh-frozen and formalin-fixed, paraffin-embedded human brain tissue.

    Science.gov (United States)

    Wang, Jian-Hua; Gouda-Vossos, Amany; Dzamko, Nicolas; Halliday, Glenda; Huang, Yue

    2013-10-01

    Both fresh-frozen and formalin-fixed, paraffin-embedded (FFPE) human brain tissues are invaluable resources for molecular genetic studies of central nervous system diseases, especially neurodegenerative disorders. To identify the optimal method for DNA extraction from human brain tissue, we compared methods on differently-processed tissues. Fragments of LRRK2 and MAPT (257 bp and 483 bp/245 bp) were amplified for evaluation. We found that for FFPE samples, the success rate of DNA extraction was greater when using a commercial kit than a laboratory-based method (successful DNA extraction from 76% versus 33% of samples). PCR amplicon size and storage period were key factors influencing the success rate of DNA extraction from FFPE samples. In the fresh-frozen samples, the DNA extraction success rate was 100% using either a commercial kit (QIAamp DNA Micro) or a laboratory-based method (sample boiling in 0.1 mol/L NaOH, followed by proteinase K digestion, and then DNA extraction using Chelex-100) regardless of PCR amplicon length or tissue storage time. Although the present results demonstrate that PCR-amplifiable genomic DNA can be extracted from both fresh-frozen and FFPE samples, fresh brain tissue is recommended for DNA extraction in future neuropathological studies.

  20. Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles

    OpenAIRE

    Park, Gyeong-Hun; Park, Ki-young; Cho, Hong-il; Lee, Sang-Min; Han, Ji Su; Won, Chong Hyun; Chang, Sung Eun; Lee, Mi Woo; Choi, Jee Ho; Moon, Kee Chan; Shin, Hyoseung; KANG, YONG JUNG; Lee, Dong Hun

    2015-01-01

    Ginseng has been shown to promote hair growth in several recent studies. However, its effects on human hair follicles and its mechanisms of action have not been sufficiently elucidated. This study aimed to investigate the hair growth-promoting effects of red ginseng extract (RGE) and its ginsenosides. The proliferative activities of cultured human hair follicles treated with RGE and ginsenoside-Rb1 were assessed using Ki-67 immunostaining. Their effects on isolated human dermal papilla cells ...

  1. Variation in Cuspal Morphology in Maxillary First Permanent Molar with Report of 3 Cusp Molar- A Prevalence Study

    Science.gov (United States)

    2016-01-01

    Introduction Human teeth has always been known for morphological variations in both the crown and root structures. The corono-morphological variations can be in the form of extra cusp or missing cusp. Permanent maxillary first molars are the biggest teeth in maxillary arch and have a high anchorage value and are known for their four cusp and five cusp patterns, if present with cusp of Carebelli. Aim The aim of this study was to determine the prevalence of cuspal variations and quantification of cusps of permanent maxillary first molar in Malwa population. Materials and Methods A total of 1249 individuals were studied at Government College of Dentistry, Indore, Madhya Pradesh, India, to evaluate the number of cusps in permanent maxillary first molars. Results Of the studied 1249 individuals, permanent maxillary first molars had five cusps in 407 (32.6%) cases while 838 (67.08%) cases had four cusp and four (0.32%) cases had three cusps. The four cases having three cusp permanent maxillary first molars were present unilaterally and only in females. Conclusion This article emphasizes the presence of permanent maxillary first molar with only three cusps in the Malwa population of India. It also reviews the literature in respect to this rare anomaly and calls for continuous and close monitoring to report such cases in the future. PMID:27790576

  2. A comparative study of two methods for the isolation of human leucocytes for DNA extraction.

    Science.gov (United States)

    Lim, L H; Ton, S H; Cheong, S K

    1990-06-01

    The 'Dextran' and the 'Buffy-coat' methods for isolation of human leucocytes for DNA extraction were compared on the basis of DNA yield from the same amounts (10 ml) of blood. Human leucocytes from a total of 11 samples were isolated using both methods for each sample after which DNA was extracted. Extracted DNA samples were treated with ribonucleases and proteinase K after which the yields were quantitated by measuring absorbance at 260 nm. The 'Buffy-coat' method yielded a mean concentration of DNA of 476.7 micrograms/ml (range: 212 to 700 micrograms/ml) while the 'Dextran' method yielded 188.4 micrograms/ml (range: 64 to 340 micrograms/ml). The difference was confirmed by subjecting the extracted DNA samples to agarose gel electrophoresis.

  3. Effect of Baliospermum montanum root extract on phagocytosis by human neutrophils

    Directory of Open Access Journals (Sweden)

    Patil Kalpana

    2009-01-01

    Full Text Available Aqueous extract of roots of Baliospermum montanum was evaluated on preliminary basis for immunomodulatory activity by studying neutrophil phagocytic function. The different concentration of (25, 50, 100 mg/ml of aqueous extract of roots of Baliospermum montanum was subjected to study its effect on different in vitro methods of phagocytosis such as neutrophil locomotion, chemotaxis, immunostimulant activity of phagocytosis of killed Candida albicans and qualitative nitroblue tetrazolium test by using human neutrophils. This preliminary study revealed that Baliospermum montanum extract has stimulated chemotactic, phagocytic and intracellular killing potency of human neutrophils at the different concentration. From the results obtained it can be observed that the aqueous extract of Baliospermum montanum stimulate cell-mediated immune system by increasing neutrophil function.

  4. 正畸牵引法辅助拔除接触下牙槽神经管的埋伏阻生牙1例报告%Orthodontic-assisted extraction of impacted lower third molar compressing to the inferior alveolar nerve: A case report

    Institute of Scientific and Technical Information of China (English)

    谢千阳; 马志贵; 钱文涛; 徐光宙; 蔡协艺; 杨驰

    2012-01-01

    Extraction of impacted lower third molar is a common procedure. Damage of the inferior alveolar nerve (IAN) is one of the severe complications, in that the roots of the tooth are often close to the nerve canal when it is impacted at a low position. There are mainly three approaches: coronectomy, partial odontoectomy and orthodontic extraction to minimize neurological risks. A case of mesioangular impacted third molar treated with improved orthodontic extraction was reported in this paper. The orthodontic treatment consisted 2 stages, up-righting and extrusion. Supported by 2011 Medical Research Project of Health Bureau of Pudong New District of Shanghai Mumcipality(PW2011D-4).%阻生下颌第三磨牙拔除是口腔颌面外科的一种常见手术.由于低位阻生的下颌第三磨牙牙根位置常与下牙槽神经(inferior alveolar nerve,IAN)关系密切,神经损伤是该手术的严重并发症之一.目前,国际上主要有3种避免IAN损伤的方法:截冠法、部分牙冠切除术和正畸牵引法.本文报告1例下颌第三磨牙近中斜位低位阻生的患者,采用改良正畸牵引法,分两步将牙根牵离神经管,牵引完成后顺利拔除患牙.

  5. Trifolium pallidum and Trifolium scabrum extracts in the protection of human plasma components.

    Science.gov (United States)

    Kolodziejczyk-Czepas, Joanna; Olas, Beata; Malinowska, Joanna; Wachowicz, Barbara; Moniuszko-Szajwaj, Barbara; Kowalska, Iwona; Oleszek, Wieslaw; Stochmal, Anna

    2013-02-01

    Clovers (genus: Trifolium) have been used in traditional medicine by many cultures, but the biological activity of the most of these plants still remains unknown. The aim of our in vitro study was to assess the antioxidative action of phenolic extracts from aerial parts of Trifolium scabrum and Trifolium pallidum in human blood plasma, exposed to oxidative stress. In the present study we also demonstrate, for the first time the effects of the tested extracts on coagulative properties and fibrinolytic activity of blood plasma. The protective properties of the examined extracts (0.5-50 μg/ml) against peroxynitrite-induced oxidative stress were estimated by the measurements of 3-nitrotyrosine, thiol groups and the thiobarbituric acid-reactive substances levels. The extracts considerably prevented the oxidative and nitrative damage to plasma proteins. Even the lowest doses of the Trifolium extracts (0.5 μg/ml) were able to markedly reduce 3-nitrotyrosine formation (by about 50%) and to increase the level of -SH groups (by about 30%), in comparison to the plasma exposed to ONOO(-) in the absence of the extracts. The protective action of all the used concentrations of the Trifolium extracts in the prevention of lipid peroxidation was also found. The tested extracts influenced neither the coagulative properties nor fibrinolytic activity of plasma. Moreover, the extracts were able to significantly reduce the inhibitory effect of ONOO(-) on fibrinolytic activity of plasma (assessed with the use of a chromogenic substrate for plasmin).

  6. Induction of sister chromatid exchanges by coal dust and tobacco snuff extracts in human peripheral lymphocytes

    Energy Technology Data Exchange (ETDEWEB)

    Tucker, J.D.; Ong, T.

    1985-01-01

    The organic solvent extracts of sub-bituminous coal dust and tobacco snuff, both together and separately, were tested for the induction of sister chromatid exchanges (SCEs) in human peripheral lymphocytes. The results indicate that these extracts induced SCEs, and that when tested together synergistically induced SCEs in two of three donors. Studies with the organic solvent extracts of all five ranks of coal indicate that the extracts of bituminous, lignite, and peat, but not anthracite, induced SCEs. Similar experiments conducted with water extracts, induced SCEs, and that anthracite was equivocal. To determine whether individuals differed in their SCE responses to coal dust extracts, lymphocytes from five donors were tested with organic solvent extracts of bituminous and sub-bituminous coal. An analysis of variance indicates that the SCE response was significantly influenced by the donor and each of the two coal extracts. The findings presented here suggest that coal dust, with or without tobacco snuff, may play a role in the elevated incidence of gastric cancer in coal miners. Because water extracts of some ranks of coal induced SCEs, there exists the possibility of adverse environmental effects due to coal leachates.

  7. Extracting Three Dimensional Surface Model of Human Kidney from the Visible Human Data Set using Free Software

    CERN Document Server

    P, Kirana Kumara

    2013-01-01

    Three dimensional digital model of a representative human kidney is needed for a surgical simulator that is capable of simulating a laparoscopic surgery involving kidney. Buying a three dimensional computer model of a representative human kidney, or reconstructing a human kidney from an image sequence using commercial software, both involve (sometimes significant amount of) money. In this paper, author has shown that one can obtain a three dimensional surface model of human kidney by making use of images from the Visible Human Data Set and a few free software packages (ImageJ, ITK-SNAP, and MeshLab in particular). Images from the Visible Human Data Set, and the software packages used here, both do not cost anything. Hence, the practice of extracting the geometry of a representative human kidney for free, as illustrated in the present work, could be a free alternative to the use of expensive commercial software or to the purchase of a digital model.

  8. Occlusal migration of the maxillary first primary molars subsequent to the loss of antagonists.

    Science.gov (United States)

    Yonezu, T; Machida, Y

    1997-08-01

    The purpose of this study was to observe the vertical changes in unopposed maxillary first primary molars longitudinally. The subjects of this study were 17 children whose lower first primary molars had to be extracted. Space closure were prevented by crown-loop space maintainers for all these children. Plaster casts were made every 4 months for 16 to 24 months after the extraction. These series of casts were standardized, and vertical changes of the maxillary first primary molars against the occlusal plane were measured using a micro-reader. The mean changes indicated that maxillary first primary molars without intercuspidation tend to drift toward the extraction space. On the control side with antagonists, the maxillary first molars seemed to move reversed to apical throughout the observation period. Accordingly, the results of this study showed new trends after the premature loss of primary molars. The vertical changes toward extraction space varied from -0.40 mm to 1.43 mm at the 16 months after extraction. Mean changes were small, but there were some individual differences in reaction, giving negative values. However, we should always be concerned about loss of arch length and also occlusal drift of unopposed teeth, because the vertical changes were greater than 1 mm at 16 months after extraction in some cases.

  9. The effect of Lamium album extract on cultivated human corneal epithelial cells (10.014 pRSV-T

    Directory of Open Access Journals (Sweden)

    Roman Paduch

    2015-01-01

    Conclusion: Selected Lamium album extracts influence human corneal epithelial cells. Generally, while not toxic, they modulate pro-inflammatory and anti-inflammatory cytokines levels, and decrease NO release by cells; moreover, ethanol and ethyl acetate extracts reduce ROS levels.

  10. 下颌第三磨牙拔除术后疼痛的临床分析及预防%Clinical analysis and prevention of the pain after extraction of mandibular third molar surgery

    Institute of Scientific and Technical Information of China (English)

    马儒

    2015-01-01

    目的:探索并分析下颌第三磨牙拔除术后疼痛的临床相关因素以及总结预防疼痛的方法,有效缓解下颌第三磨牙拔除术后疼痛。方法将该院2014年2—7月收治的120例需行下颌第三磨牙拔除术患者作为研究对象,随机分为观察组和对照组,每组60例,对照组患者进行常规的手术治疗方法以及基础护理方法,观察组患者,在常规治疗方法的基础上,实施相关的临床路径干预办法(包括术前干预、护理干预及药物干预),探索下颌第三磨牙拔除术后疼痛的相关性因素,并比较两组患者的干预前后的疼痛评分情况,最后进行分析评价。结果术后1d患者的疼痛与手术切口的局部感染因素(牙齿龋坏等)以及女性月经周期(月经期后2周内)相关;分别对两组患者术后当天、1 d、2 d、3 d的疼痛情况分别进行VAS评分,经对比后,观察组的评分均明显低于对照组,且两组比较差异有统计学意义(P<0.05)。结论对于以疼痛为主要术后症状的下颌第三磨牙拔除术,通过术前干预、药物及护理干预方法,可有效降低患者的VAS评分,缓解患者术后疼痛,提高患者术后生活质量,对改善患者预后具有积极作用,因此上述的干预方法可在临床路径中推广使用。%Objective To explore the post-operative pain related medical factors in the mandibular third molar surgery and sum-marizes the prevention of pain, then effectively relieve the postoperative pain after the mandibular third molar surgery. Methods Choose 120 cases required for mandibular third molar surgery patients as the research object in my hospital ,whose entered the hospital from February to July in 2014 .Then the patients were randomly divided into observation group and control group and 60 cases in each group. The control group were operated by the routine treatment and nursing methods, the observation group

  11. Cytotoxic effect of Argentine medicinal plant extracts on human hepatocellular carcinoma cell line.

    Science.gov (United States)

    Ruffa, M J; Ferraro, G; Wagner, M L; Calcagno, M L; Campos, R H; Cavallaro, L

    2002-03-01

    Methanolic extracts from Achyrocline satureioides (Dc.) Lam, Aristolochia macroura Gomez, Lithraea molleoides (Vell.) Engl., Schinus molle L., unlike those from Celtis spinosa Spreng, Chenopodium ambrosioides L., Petiveria alliacea L., and Plantago major L. showed cytotoxic activity against a human hepatocellular carcinoma cell line, Hep G2. Schinus molle L. was the most active (IC50=50+/-7 microg/ml). These results call for further studies of these extracts.

  12. Oxygen-independent killing of Bacteroides fragilis by granule extracts from human polymorphonuclear leukocytes.

    OpenAIRE

    Wetherall, B L; Pruul, H; McDonald, P J

    1984-01-01

    Granule proteins from human neutrophils were prepared by extraction with acetate, and their antibacterial activity against Bacteroides fragilis was determined. Activity was highly dependent on pH; greatest killing occurred at the most acid pH tested (pH 5.0). Optimum activity was observed at physiological ionic strength and low bacterial numbers. Killing was inhibited by incubation temperatures of less than 37 degrees C. Eight times more extract was required to kill 50% of stationary-phase ba...

  13. White Tea extract induces lipolytic activity and inhibits adipogenesis in human subcutaneous (pre-adipocytes

    Directory of Open Access Journals (Sweden)

    Wenck Horst

    2009-05-01

    Full Text Available Abstract Background The dramatic increase in obesity-related diseases emphasizes the need to elucidate the cellular and molecular mechanisms underlying fat metabolism. To investigate how natural substances influence lipolysis and adipogenesis, we determined the effects of White Tea extract on cultured human subcutaneous preadipocytes and adipocytes. Methods For our in vitro studies we used a White Tea extract solution that contained polyphenols and methylxanthines. Utilizing cultured human preadipocytes we investigated White Tea extract solution-induced inhibition of triglyceride incorporation during adipogenesis and possible effects on cell viability. In vitro studies on human adipocytes were performed aiming to elucidate the efficacy of White Tea extract solution to stimulate lipolytic activity. To characterize White Tea extract solution-mediated effects on a molecular level, we analyzed gene expression of essential adipogenesis-related transcription factors by qRT-PCR and determined the expression of the transcription factor ADD1/SREBP-1c on the protein level utilizing immunofluorescence analysis. Results Our data show that incubation of preadipocytes with White Tea extract solution significantly decreased triglyceride incorporation during adipogenesis in a dose-dependent manner (n = 10 without affecting cell viability (n = 10. These effects were, at least in part, mediated by EGCG (n = 10, 50 μM. In addition, White Tea extract solution also stimulated lipolytic activity in adipocytes (n = 7. Differentiating preadipocytes cultivated in the presence of 0.5% White Tea extract solution showed a decrease in PPARγ, ADD1/SREBP-1c, C/EBPα and C/EBPδ mRNA levels. Moreover, the expression of the transcription factor ADD1/SREBP-1c was not only decreased on the mRNA but also on the protein level. Conclusion White Tea extract is a natural source that effectively inhibits adipogenesis and stimulates lipolysis-activity. Therefore, it can be utilized to

  14. Space loss following premature loss of primary second molars.

    Science.gov (United States)

    Alnahwi, Hassan H; Donly, Kevin J; Contreras, Claudia I

    2015-01-01

    This study was designed to evaluate the amount of space loss (SL) caused by premature loss of primary second molars, determine whether the eruption status of permanent first molars is an important factor in the amount of SL, and evaluate the effectiveness of space maintainers (SMs) in SL prevention. SL associated with 100 prematurely extracted primary second molars was evaluated in 87 healthy patients. Teeth were divided into groups based on the use of SMs (36 with SM and 64 without SM). Bitewing and periapical radiographs taken before extraction and 6, 12, 24, 36, and 48 months after extraction were used to determine the amount of SL. Not every patient attended every recall appointment, so the sample size varied at different evaluation times. The most significant amount of SL occurred in the first 12 months after extraction. In patients who did not use an SM, at 6 months there was a mean SL of 2.12 mm (SD, 1.65 mm) and at 12 months there was a mean of 4.02 mm (SD, 1.65), with significantly more SL in the first 6 months (P 0.05). When patients without an SM were grouped by the eruption status of the permanent first molar, there was significantly more SL in the groups with unerupted first molars than there was in the groups with erupted first molars at both 6 months (P < 0.001) and 12 months (P < 0.05). At both 6 and 12 months, the amount of SL in patients who had an SM (n = 13 and n = 14, respectively) was not significantly different from the amount of SL in those who did not have an SM (n = 33 and n = 23, respectively). SMs should be placed as soon as possible following tooth extraction to prevent undue SL. Placement of an SM a year or more after extraction has minimal benefit, since most SL takes place within the first year. SL does occur even when SMs are used.

  15. Extraction of DNA from human embryos after long-term preservation in formalin and Bouin's solutions.

    Science.gov (United States)

    Nagai, Momoko; Minegishi, Katsura; Komada, Munekazu; Tsuchiya, Maiko; Kameda, Tomomi; Yamada, Shigehito

    2016-05-01

    The "Kyoto Collection of Human Embryos" at Kyoto University was begun in 1961. Although morphological analyses of samples in the Kyoto Collection have been performed, these embryos have been considered difficult to genetically analyze because they have been preserved in formalin or Bouin's solution for 20-50 years. Owing to the recent advances in molecular biology, it has become possible to extract DNA from long-term fixed tissues. The purpose of this study was to extract DNA from wet preparations of human embryo samples after long-term preservation in fixing solution. We optimized the DNA extraction protocol to be suitable for tissues that have been damaged by long-term fixation, including DNA-protein crosslinking damage. Diluting Li2 CO3 with 70% ethanol effectively removed picric acid from samples fixed in Bouin's solution. Additionally, 20.0 mg/mL proteinase was valuable to lyse the long-term fixed samples. The extracted DNA was checked with PCR amplification using several sets of primers and sequence analysis. The PCR products included at least 295- and 838-bp amplicons. These results show that the extracted DNA is applicable for genetic analyses, and indicate that old embryos in the Kyoto Collection should be made available for future studies. The protocol described in this study can successfully extract DNA from old specimens and, with improvements, should be applicable in research aiming to understand the molecular mechanisms of human congenital anomalies. © 2015 Japanese Teratology Society.

  16. 局部应用地塞米松预防下颌阻生智齿拔除术后并发症的疗效观察%Effects of local injection of dexamethasone in treatment of complications after extraction of mandibular third molars

    Institute of Scientific and Technical Information of China (English)

    赖军; 董正谋; 杜忠洪

    2014-01-01

    目的:观察局部注射地塞米松预防下颌第三磨牙阻生拔除术后面部肿胀度和疼痛程度的临床疗效。方法:筛选2010年3月至2014年2月102例18~25周岁的、需拔除的下颌阻生第三磨牙患者,分成实验组和对照组,每组51例,实验组在拔牙区软组织内局部注射地塞米松。术后第1天分别对两组患者观察面部肿胀度和测试疼痛程度。结果:实验组患者拔牙术后面部肿胀率明显低于对照组。术后两组的疼痛程度没有显著差异。结论:术前术区局部注射地塞米松,可以有效减少下颌阻生第三磨牙拔除术后的面部肿胀程度,有较好的临床疗效。%Objective:To investigate the effects of dexamethasone injection preoperatively on facial swelling and pain af-ter lower impacted third molar surgery. Method:102 mandibular impacted third molars needed surgical extraction were di-vided into experimental group and control group from March 2010 to February 2014. Patients in experimental group received partially injection of dexamethasone on soft tissue before the surgical extraction. Clinical assessment of the pain and facial swelling was measured at first day postoperatively. Result:Facial swelling score were lower obviously in experimental group, but there were not significant differences in assessment of the pain between experimental group and control groups. Con-clusion:With partially injection of dexamethasone on soft tissue before the surgical extraction,it is effective to improve the complication of facial swelling after mandibular impacted third molar surgery.

  17. Neuroprotective Effect of Human Adipose Stem Cell-Derived Extract in Amyotrophic Lateral Sclerosis.

    Science.gov (United States)

    Jeon, Gye Sun; Im, Wooseok; Shim, Yu-Mi; Lee, Mijung; Kim, Myung-Jin; Hong, Yoon-Ho; Seong, Seung-Yong; Kim, Manho; Sung, Jung-Joon

    2016-04-01

    Amyotrophic lateral sclerosis (ALS) is a devastating human neurodegenerative disease. The precise pathogenic mechanisms of the disease remain uncertain, and as of yet, there is no effective cure. Human adipose stem cells (hASC) can be easily obtained during operative procedures. hASC have a clinically feasible potential to treat neurodegenerative disorders, since cytosolic extract of hASC contain a number of essential neurotrophic factors. In this study, we investigated effects of hASC extract on the SOD1 G93A mouse model of ALS and in vitro test. Administration of hASC extract improved motor function and prolonged the time until symptom onset, rotarod failure, and death in ALS mice. In the hASC extracts group, choline acetyltransferase immunostaining in the ventral horn of the lumbar spinal cord showed a large number of motor neurons, suggesting normal morphology. The neuroprotective effect of hASC extract in ALS mice was also suggested by western blot analysis of spinal cord extract from ALS mice and in vitro test. hASC extract treatment significantly increased expression of p-Akt, p-CREB, and PGC-1α in SOD1 G93A mouse model and in vitro test. Our results indicated that hASC extract reduced apoptotic cell death and recovered mutant SOD1-induced mitochondrial dysfunction. Moreover, hASC extract reduced mitochondrial membrane potential. In conclusion, we have demonstrated, for the first time, that hASC extract exert a potential therapeutic action in the SOD1 G93A mouse model of ALS and in vitro test. These findings suggest that hASC hold promise as a novel therapeutic strategy for treating ALS.

  18. Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

    Institute of Scientific and Technical Information of China (English)

    Nurfariza; Ahmad; Roslen; Nur; Aizura; Mat; Alewi; Hadji; Ahamada; Mohammad; Syaiful; Bahari; Abdull; Rasad

    2014-01-01

    Objective:To screen the cytotoxic activity of Melasloma malabathricum(M,malubathricum)against human breast carreer cell line(MCF-7)in vitro.Methods:A three steps extraction protocol using n-hexane,chloroform and methanol as the solvents systems was carried out on leaves,stems and flowers of M.nalabathricum.Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations(100μg/mL,50μg/mL,25μg/mL,123μg/rnL and 6.25μg/mL).The evaluation of cell growth was determined using methylene blue assay.Results:Methanol extract from the leaves showed significant anticancer activity against MCF-7cell lines with the TC50value of 7.14μg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line,with the IC50value of 33.63μg/mL and 45.76μg/mL respectively after 72 h of treatment.Conclusions:The extracts from leaves and flowers of M.nulabatkricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50at 7.14μg/mL while the extracts from stems showed less growth inhibition activity.

  19. Effects of Two Different Rhodiola rosea Extracts on Primary Human Visceral Adipocytes

    Directory of Open Access Journals (Sweden)

    Elena Pomari

    2015-05-01

    Full Text Available Rhodiola rosea (Rro has been reported to have various pharmacological properties, including anti-fatigue, anti-stress and anti-inflammatory activity. It is also known to improve glucose and lipid metabolism, but the effects of Rhodiola rosea on adipocyte differentiation and metabolism are not still elucidated. In this study the anti-adipogenic and lipolytic activity of two extracts of Rhodiola rosea, containing 3% salidroside (RS or 1% salidroside and 3% rosavines (RR on primary human visceral adipocytes was investigated. Pre-adipocytes were analyzed after 10 and 20 days of treatment during differentiation and after 7 days of treatment when they reached mature shape. The RS extract significantly induced higher apoptosis and lipolysis in comparison to control cells and to RR extract. In contrast, RR extract significantly reduced triglyceride incorporation during maturation. Differentiation of pre-adipocytes in the presence of RS and RR extracts showed a significant decrease in expression of genes involved in adipocyte function such as SLC2A4 and the adipogenic factor FGF2 and significant increase in expression of genes involved in inhibition of adipogenesis, such as GATA3, WNT3A, WNT10B. Furthermore RR extract, in contrast to RS, significantly down-regulates PPARG, the master regulator of adipogenesis and FABP4. These data support the lipolytic and anti-adipogenetic activity of two different commercial extracts of Rhodiola rosea in primary human visceral pre-adipocytes during differentiation.

  20. Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

    Institute of Scientific and Technical Information of China (English)

    Nurfariza Ahmad Roslen; Nur Aizura Mat Alewi; Hadji Ahamada

    2014-01-01

    Objective: To screen the cytotoxic activity of Melastoma malabathricum (M. malabathricum) against human breast cancer cell line (MCF-7) in vitro. Methods: A three steps extraction protocol using n-hexane, chloroform and methanol as the solvents systems was carried out on leaves, stems and flowers of M. malabathricum. Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations (100 µg/mL, 50 µg/mL, 25 µg/mL, 12.5 µg/mL and 6.25 µg/mL). The evaluation of cell growth was determined using methylene blue assay.Results:Methanol extract from the leaves showed significant anticancer activity against MCF-7 cell lines with the IC50 value of 7.14 µg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line with the IC50 value of 33.63 µg/mL and 45.76 µg/mL respectively after 72 h of treatment.Conclusions:The extracts from leaves and flowers of M. malabathricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50 at 7.14 µg/mL while the extracts from stems showed less growth inhibition activity.

  1. Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation

    Directory of Open Access Journals (Sweden)

    Dehelean Cristina A

    2012-11-01

    Full Text Available Abstract Background Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition. Results New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%. Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma, A2780 (ovarian carcinoma, HeLa (cervix adenocarcinoma and MCF7 (breast adenocarcinoma, by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice. Conclusions The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.

  2. Platelet-Derived Growth Factor-Mediated Guided Bone Regeneration in Immediate Implant Placement in Molar Sites with Buccal Bone Defects.

    Science.gov (United States)

    Santana, Ronaldo B; Santana, Carolina Mm; Dibart, Serge

    2015-01-01

    This study compared the clinical outcomes of recombinant human platelet-derived growth factor BB and beta-tricalcium phosphate (rhPDGF-BB/βTCP) with guided bone regeneration (GBR) in immediate implant placement in molar extraction sockets with buccal bone defects versus conventional implant placement. Twenty-eight implants were placed in fourteen patients. Clinical and radiographic evaluations assessed peri-implant soft and hard tissue parameters after 12 months. No implants were lost during the 1-year observation period, yielding a survival rate of 100%. Similar clinical and radiographic parameters were observed for both treatment groups. Use of rhPDGF-BB/βTCP and GBR in immediate implants in molars was as successful as conventional implant placement in fully healed extraction sites.

  3. Curcuminoids extract, hydrolyzed collagen and green tea extract synergically inhibit inflammatory and catabolic mediator's synthesis by normal bovine and osteoarthritic human chondrocytes in monolayer.

    Science.gov (United States)

    Comblain, Fanny; Sanchez, Christelle; Lesponne, Isabelle; Balligand, Marc; Serisier, Samuel; Henrotin, Yves

    2015-01-01

    The main objective of this study was to assess the in vitro effects of curcuminoids extract, hydrolyzed collagen and green tea extract in normal bovine chondrocytes and osteoarthritic human chondrocytes cultured in monolayer. This study also investigated the synergic or additive effects of these compounds. Enzymatically isolated primary bovine or human chondrocytes were cultured in monolayer until confluence and then incubated for 24 hours or 48 hours in the absence or in the presence of interleukin-1β and with or without curcuminoids extract, hydrolyzed collagen or green tea extract, added alone or in combination, at different concentrations. Cell viability was neither affected by these compounds, nor by interleukin 1β. In the absence of interleukin-1β, compounds did not significantly affect bovine chondrocytes metabolism. In human chondrocytes and in the absence of interleukin 1β, curcuminoids extract alone or in combination with hydrolyzed collagen and green tea extract significantly inhibited matrix metalloproteinase-3 production. In interleukin-1β-stimulated bovine chondrocytes, interleukin-6, inducible nitric oxide synthase, cyclooxygenase2, matrix metalloproteinase 3, a disintegrin and metalloproteinase with thrombospondin type I motifs 4 and a disintegrin and metalloproteinase with thrombospondin type I motifs 5 expressions were decreased by curcuminoids extract alone or in combination with hydrolyzed collagen and green tea extract. The combination of the three compounds was significantly more efficient to inhibit interleukin-1β stimulated matrix metalloproteinase-3 expression than curcuminoids extract alone. In interleukin-1β-stimulated human chondrocytes, nitric oxide, interleukin-6 and matrix metalloproteinase 3 productions were significantly reduced by curcuminoids extract alone or in combination with hydrolyzed collagen and green tea extract. These findings indicate that a mixture of curcuminoids extract, hydrolyzed collagen and green tea

  4. Third molar complications requiring hospitalization.

    Science.gov (United States)

    Kunkel, Martin; Morbach, Thomas; Kleis, Wilfried; Wagner, Wilfried

    2006-09-01

    The aim of this study was to describe demographic and clinical patterns of subjects hospitalized with complications associated with third molars (M3). The investigation was designed as a prospective cohort study composed of subjects admitted to hospital for management of M3-associated complications. The predictor variable was "clinical status of the M3" defined as (A) prophylactic M3 removal, (B) nonelective M3 removal, or (C) M3 present at the time of admission. Outcome variables were infection parameters, treatment costs, length of hospital stay, and days of disability. Postoperative complications (A and B) were compared to complications based on pericoronitis (C). Complications due to prophylactic removal (A) were compared to those arising from pericoronitis or from the removal of symptomatic teeth (B and C). From January 2003 to December 2004, 45 deep space infections, 6 mandibular fractures, 2 lingual nerve injuries, 1 parapharyngeal tooth luxation, and 1 osteomyelitis were noticed. Fifteen complications resulted from prophylactic surgery (A), 25 from nonelective removal (B), and 15 from pericoronitis (C). Direct treatment costs were 147,000 euro (A: 42,000 euro; B: 74,000 euro; C: 31,000 euro). In 10 of the 15 patients of group C, deep space involvement resulted immediately from the first episode of pericoronitis. Neither clinical markers of infection nor economic parameters showed significant differences between the groups. Within the catchment area of our institution, the majority of third molar-related hospitalizations resulted from diseased third molars or their removal.

  5. [Determination of amphetamines in human urine using microwave extraction-gas chromatography].

    Science.gov (United States)

    Wang, Jifen; Sun, Hongfeng; Ye, Nengsheng; Gu, Xuexin; Li, Wenjun; Li, Ying

    2008-03-01

    A method has been developed for the determination of methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) in human urine using microwave extraction-gas chromatography(GC). To improve the extraction efficiency, experimental parameters of the extraction, including extraction solvent and its amount, pH value of the urine sample, extraction time and temperature were investigated. The optimal conditions were as follows: the pH value of urine sample at pH 12, cyclohexane as extraction solvent, extraction at 40 degrees C for 10 min. The average recoveries of MA, MDA and MDMA with this extraction method were 92.25%, 85.94% and 91.50%, the relative standard deviations were 5.5%, 5.5% and 6.1%(n = 5), and the limits of detection were 10, 20 and 20 ng/mL, respectively. Using this method, MA, MDA and MDMA need not be derivatized and can be separated from the matrix. The results indicate that the developed method is rapid, accurate and sensitive, and can be used for the simultaneous determination of MA, MDA and MDMA in urine samples.

  6. Evaluation of antimicrobial properties of four plant extracts against human pathogens

    Institute of Scientific and Technical Information of China (English)

    Anjali Rawani; Sudin Pal; GoutamChandra

    2011-01-01

    Objective: To investigate the antibacterial activity of the extracts of Alternanthera philoxeroides (A. philoxeroides), Plumeria obtusa (P. obtusa), Polyalthia cerasoides (P. cerasoides) and Ixoraacuminate (I. acuminate) against human pathogens. Methods: Aqueous and chloroform: methanol (1:1) extracts of the dried leaf of A. philoxeroides, flowers of P. obtusa, fruits of P. cerasoides and flowers of I. acuminate were tested in vitro by the disk diffusion method against four bacterial strains, namely, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonasaeruginosa. Susceptibility of four reference bacterial strains to some antibiotics in nutrient agar was also tested. Minimal inhibitory concentration (MIC) values were determined and qualitative phytochemical analysis of the crude extract of the tested plant parts was done. Results: Both the aqueous and the chloroform: methanol (1:1) extracts of P. cerasoides showed the strongest activity, followed by flowers of P. obtusa, leaves of A. philoxeroides and flowers of I. acuminate. Aqueous extracts of all the plant parts appeared to have less antibacterial activity than the chloroform:methanol (1:1) extracts. The result of phytochemical analysis of the crude extract of the tested plants showed that flavonoid was absent from all plant parts whereas steroid was present in all tested plant parts. Conclusions: The results support that these plant extracts can be used for the treatment of bacterial diseases.

  7. Wound healing potential of Spirulina platensis extracts on human dermal fibroblast cells

    Science.gov (United States)

    Syarina, Pauzi Nur Aimi; Karthivashan, Govindarajan; Abas, Faridah; Arulselvan, Palanisamy; Fakurazi, Sharida

    2015-01-01

    Blue-green alga (Spirulina platensis) is a well renowned nutri-supplement due to its high nutritional and medicinal properties. The aim of this study was to examine the wound healing efficiency of Spirulina platensis at various solvent extracts using in vitro scratch assay on human dermal fibroblast cells (HDF). Various gradient solvent extracts (50 μg/ml of methanolic, ethanolic and aqueous extracts) from Spirulina platensis were treated on HDF cells to acquire its wound healing properties through scratch assay and in this investigation we have used allantoin, as a positive control to compare efficacy among the phytoextracts. Interestingly, aqueous extract were found to stimulate proliferation and migration of HDF cells at given concentrations and enhanced closure rate of wound area within 24 hours after treatment. Methanolic and ethanolic extracts have shown proliferative effect, however these extracts did not aid in the migration and closure of wound area when compared to aqueous extract. Based on phytochemical profile of the plant extracts analyzed by LC-MS/MS, it was shown that compounds supposedly involved in accelerating wound healing are cinnamic acid, narigenin, kaempferol, temsirolimus, phosphatidylserine isomeric derivatives and sulphoquinovosyl diacylglycerol. Our findings concluded that blue-green algae may pose potential biomedical application to treat various chronic wounds especially in diabetes mellitus patients. PMID:27004048

  8. Interaction mechanism between green tea extract and human α-amylase for reducing starch digestion.

    Science.gov (United States)

    Miao, Ming; Jiang, Bo; Jiang, Huan; Zhang, Tao; Li, Xingfeng

    2015-11-01

    This study evaluated the inhibitory effects of the green tea extract on human pancreatic α-amylase activity and its molecular mechanism. The green tea extract was composed of epicatechin (59.2%), epigallocatechin gallate (14.6%) and epicatechin gallate (26.2%) as determined by HPLC analysis. Enzyme activity measurement showed that % inhibition and IC50 of the green tea extract (10%, based on starch) were 63.5% and 2.07 mg/ml, respectively. The Michaelis-Menten constant remained unchanged but the maximal velocity decreased from 0.43 (control) to 0.07 mg/(ml × min) (4 mg/ml of the green tea extract), indicating that the green tea extract was an effective inhibitor against α-amylase with a non-competitive mode. The fluorescence data revealed that the green tea extract bound with α-amylase to form a new complex with static quenching mechanism. Docking study showed the epicatechin gallate in the green tea extract presented stronger affinity than epigallocatechin gallate, with more number of amino acid residues involved in amylase binding with hydrogen bonds and Van der Waals forces. Thus, the green tea extract could be used to manipulate starch digestion for potential health benefits.

  9. Dual effects of Ginkgo biloba leaf extract on human red blood cells.

    Science.gov (United States)

    He, Jing; Lin, Juan; Li, Jing; Zhang, Jian-Hong; Sun, Xue-Min; Zeng, Cheng-Ming

    2009-02-01

    Extracts from the leaves of Ginkgo biloba have been used in Chinese medicine for thousands of years. Today, various standardized preparations from G. biloba leaf extract have been developed. G. biloba leaf extract, which contains flavonoids and terpenoids as the major biologically active components, has become one of the most popular and commonly used herbal remedies due to its wide spectrum of beneficial effects on health. In this study, we investigated the effects of G. biloba leaf extract on the properties of human red blood cells in the presence and absence of amyloid peptide (Abeta25-35), peroxide and hypotonic stress. The results suggest that G. biloba leaf extract has a dual action, both protective and disruptive, on red blood cells, depending on whether an exogenous stress is present. G. biloba leaf extract has a protective role on red blood cells against Abeta- and hypotonic pressure-induced haemolysis, peroxide-induced lipoperoxidation, as well as glutathione consumption and methaemoglobin formation. On the other hand, G. biloba leaf extract also exhibited damage to red blood cells by increasing cell fragility, changing cellular morphology and inducing glutathione consumption and methaemoglobin formation, especially when applied at high doses. These anti- and pro-oxidative activities of polyphenolic substances are thought to be involved in the dual function of G. biloba leaf extract. The results of this study suggest that high doses of herbal remedies and dietary supplements can be toxic to cells.

  10. Wound healing potential of Spirulina platensis extracts on human dermal fibroblast cells.

    Science.gov (United States)

    Syarina, Pauzi Nur Aimi; Karthivashan, Govindarajan; Abas, Faridah; Arulselvan, Palanisamy; Fakurazi, Sharida

    2015-01-01

    Blue-green alga (Spirulina platensis) is a well renowned nutri-supplement due to its high nutritional and medicinal properties. The aim of this study was to examine the wound healing efficiency of Spirulina platensis at various solvent extracts using in vitro scratch assay on human dermal fibroblast cells (HDF). Various gradient solvent extracts (50 μg/ml of methanolic, ethanolic and aqueous extracts) from Spirulina platensis were treated on HDF cells to acquire its wound healing properties through scratch assay and in this investigation we have used allantoin, as a positive control to compare efficacy among the phytoextracts. Interestingly, aqueous extract were found to stimulate proliferation and migration of HDF cells at given concentrations and enhanced closure rate of wound area within 24 hours after treatment. Methanolic and ethanolic extracts have shown proliferative effect, however these extracts did not aid in the migration and closure of wound area when compared to aqueous extract. Based on phytochemical profile of the plant extracts analyzed by LC-MS/MS, it was shown that compounds supposedly involved in accelerating wound healing are cinnamic acid, narigenin, kaempferol, temsirolimus, phosphatidylserine isomeric derivatives and sulphoquinovosyl diacylglycerol. Our findings concluded that blue-green algae may pose potential biomedical application to treat various chronic wounds especially in diabetes mellitus patients.

  11. Methanolic Extract of Plumbago Zeylanica - A Remarkable Antibacterial Agent Against Many Human and Agricultural Pathogens

    Directory of Open Access Journals (Sweden)

    Mukesh Kumar Singh

    2017-03-01

    Full Text Available Objectives: The current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of Plumbago zeylanica. Methods: The stems, leaves, and whole plants were air dried and extracted with methanol by using a Soxhlet extractor for 72 hours at 55 - 60°C. The antimicrobial activities were determined from the zones of inhibition, which were measured by using the agar well diffusion method, and the cytotoxicity assays were performed using the 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay method. Results: The methanolic extracts of the stem and the leaves of Plumbago zeylanica were tested against six bacterial species and nine fungal species, and both extracts showed antimicrobial activity in a dose-dependent manner. The leaf extract of Plumbago zeylanica showed maximum antimicrobial activity against both Staphylococcus aureus sub sp aureus and Fusarium oxysporum. The stem extract was found to be more antimicrobial against the Pseudomonas aeruginosa and the Penicillium expansum species. MTT assays were used to test the cytotoxicity of the whole plant extract in the HCT-116 and the K-562 cell lines, and that extract was shown to have weak cytotoxicity in both cell lines. Conclusion: In the present study, the methanolic stem extracts of Plumbago zeylanica were found to possess remarkable antibacterial activities against many human and agricultural pathogens. The extracts were also found to possess significant antifungal activities, but the antifungal activities were less than the antibacterial activities. Finally, the extracts were found to have weak cytotoxicities in the HCT-116 and the K-562 cell lines.

  12. Methanolic Extract of Plumbago Zeylanica - A Remarkable Antibacterial Agent Against Many Human and Agricultural Pathogens

    Science.gov (United States)

    Pandey, Ajit; Sawarkar, Hemant; Gupta, Anshita; Gidwani, Bina; Dhongade, Hemant; Tripathi, Dulal Krishna

    2017-01-01

    Objectives: The current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of Plumbago zeylanica. Methods: The stems, leaves, and whole plants were air dried and extracted with methanol by using a Soxhlet extractor for 72 hours at 55 - 60°C. The antimicrobial activities were determined from the zones of inhibition, which were measured by using the agar well diffusion method, and the cytotoxicity assays were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. Results: The methanolic extracts of the stem and the leaves of Plumbago zeylanica were tested against six bacterial species and nine fungal species, and both extracts showed antimicrobial activity in a dose-dependent manner. The leaf extract of Plumbago zeylanica showed maximum antimicrobial activity against both Staphylococcus aureus sub sp aureus and Fusarium oxysporum. The stem extract was found to be more antimicrobial against the Pseudomonas aeruginosa and the Penicillium expansum species. MTT assays were used to test the cytotoxicity of the whole plant extract in the HCT-116 and the K-562 cell lines, and that extract was shown to have weak cytotoxicity in both cell lines. Conclusion: In the present study, the methanolic stem extracts of Plumbago zeylanica were found to possess remarkable antibacterial activities against many human and agricultural pathogens. The extracts were also found to possess significant antifungal activities, but the antifungal activities were less than the antibacterial activities. Finally, the extracts were found to have weak cytotoxicities in the HCT-116 and the K-562 cell lines.

  13. New quantitative classification of the anatomical relationship between impacted third molars and the inferior alveolar nerve.

    Science.gov (United States)

    Wang, Wei-Quan; Chen, Michael Y C; Huang, Heng-Li; Fuh, Lih-Jyh; Tsai, Ming-Tzu; Hsu, Jui-Ting

    2015-12-07

    Before extracting impacted lower third molars, dentists must first identify the spatial relationship between the inferior alveolar nerve (IAN) and an impacted lower third molar to prevent nerve injury from the extraction. Nevertheless, the current method for describing the spatial relationship between the IAN and an impacted lower third molar is deficient. Therefore, the objectives of this study were to: (1) evaluate the relative position between impacted lower third molars and the IAN; and (2) investigate the relative position between impacted lower third molars and the IAN by using a cylindrical coordinate system. From the radiology department's database, we selected computed tomography images of 137 lower third molars (from 75 patients) requiring removal and applied a Cartesian coordinate system by using Mimics, a medical imaging software application, to measure the distribution between impacted mandibular third molars and the IAN. In addition, the orientation of the lower third molar to the IAN was also measured, but by using a cylindrical coordinate system with the IAN as the origin. According to the Cartesian coordinate system, most of the IAN runs through the inferior side of the third molar (78.6 %), followed by the lingual side (11.8 %), and the buccal side (8.9 %); only 0.7 % is positioned between the roots. Unlike the Cartesian coordinate system, the cylindrical coordinate system clearly identified the relative position, r and θ, between the IAN and lower third molar. Using the cylindrical coordinate system to present the relationship between the IAN and lower third molar as (r, θ) might provide clinical practitioners with a more explicit and objective description of the relative position of both sites. However, comprehensive research and cautious application of this system remain necessary.

  14. In vitro anti-proliferative activity of clove extract on human gastric carcinoma

    Directory of Open Access Journals (Sweden)

    A. Karimi

    2017-10-01

    Full Text Available Background and objectives: Cancer cell resistance to common chemotherapy agents is on rise. Plants are considered valuable sources of herbal drugs for cancer therapy. The present study was conducted to investigate the in vitro antioxidant, anti-proliferative, and apoptosis-inducing properties of clove (Syzygium aromaticum L. extract in human gastric carcinoma (AGS. Methods: Crude ethanol extract of S. aromaticum dried buds was prepared and  in vitro anti-proliferative effects of the extract on AGS and normal Human dermal fibroblasts (HDF cell lines were studied by MTT assay. To examine apoptosis induction, AGS cells were incubated with IC50 concentrations of the extract, stained with propidium iodide (PI and annexin V-fluorescein isothiocyanate (FITC, and analyzed by flow cytometry. Antioxidant activity and total phenolics and flavonoids contents were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH assay, Folin-Ciocalteu method, and aluminum chloride colorimetric method, respectively. Results: The IC50 of DPPH and total phenolics and flavonoids contents of the extract were 10.05±1.93 μg/mL, 225.6±40 mg GAE/g, and 29.30±2.35 mgRUT/g, respectively. The IC50 of the extract against HDFs was 649 µg/mL, higher than AGS cells, which was 118.7 g/mL at 48 h after treatment. Flow cytometric analysis showed that the extract induced cell apoptosis. Conclusions: Crude ethanol S. aromaticum extract had high total phenolics content, and suppressed the proliferation of human gastric cancer cells, likely due to apoptosis induction. Further studies should be conducted to determine the mechanisms of its anticancer effects.

  15. Preventive effects of tamarind seed coat extract on UVA-induced alterations in human skin fibroblasts.

    Science.gov (United States)

    Phetdee, Khemjira; Rakchai, Racharat; Rattanamanee, Kwanchai; Teaktong, Thanasak; Viyoch, Jarupa

    2014-01-01

    One of the most damaging actions on skin is from solar radiation, particularly from its ultraviolet (UV) component, through the formation of oxidative species. Thus, an antioxidant strategy that prevents the formation of these oxidants could form the basis of an efficacious cutaneous protectant. Many herbal materials contain antioxidant polyphenols, and this study assessed the possibility that tamarind seed coat extract could fulfill this role. An alcoholic extract of the tamarind (Tamarindus indica L.) seed coat showed stronger antioxidant activity (2,2-diphenyl-1-picrylhydrazyl inhibition, EC(50) = 12.9 μg/ml) than L-ascorbic acid (EC(50) = 22.9 μg/ml) and α-tocopherol (EC(50) = 29.3 μg/ml). In cultured fibroblasts taken from human skin, hydrogen peroxide (100-1000 μM) damaged 62-92% of the cells compared to only 35-47% when the cells were preincubated in extract (200 μg/ml) for 24 h. UVA (40 J/cm2) irradiation of human fibroblasts damaged 25% of the cells but the death rate was reduced to 10% with extract. UV irradiation increased the proportion of cells arrest in G(0)/G(1) phase (from 59% to 78%) but this was largely prevented by the extract (64%), according to flow cytometry. Intracellular total glutathione of UVA-irradiated cells pretreated with the extract increased to 10-25% compared to the non-pretreated group at 24-72 h after irradiation. Fibroblasts typically increased matrix metalloproteinase-1 secretion after photodamage, and this is prevented by the extract. This is the first report showing that tamarind seed coat extract is an antioxidant and can protect human skin fibroblasts from cellular damage produced by UVA and thus may form the foundation for an antiaging cosmetic.

  16. Influence of low temperature storage on the properties of human placenta extracts

    Directory of Open Access Journals (Sweden)

    Ekaterina D. Rozanova

    2012-06-01

    Full Text Available Objective: This research represents the results of comparative investigations of the composition and biological activity of aqueous-saline extracts, obtained from fresh and stored at –196ºC human placental tissues. Methods: The studying of quantitative composition of proteins in human placenta extract (HPE was performed with gel-chromatography method. Content of lipid peroxidation products and catalase activity in the extracts were evaluated using spectrophotometric assay. Biological activity of extracts was evaluated by studying the modifications of structural and functional parameters of erythrocytes after exposure with the extracts. Results: Our researches have shown that properties of HPE from the stored at –196ºС tissues slightly differ from those of fresh tissues. In extract of frozen tissues the content of high-molecular proteins is relatively higher. Content of malonyldialdehyde in extracts of the tissues stored at –196ºC does not change. Catalase activity decreases. For evaluation of biological effect of the extract on erythrocytes we have analyzed the following structural and functional parameters of red blood cells: spontaneous hemolysis, osmotic, and low-pH resistances, which characterize mechanical properties and stability of cellular membranes; relative content of main forms of hemoglobin (oxy-, deoxy-, and met-Hb, which elucidate the functioning of biochemical systems; and cytosol microviscosity, which reflects structure-dynamical state, determined, first of all, by interaction of cytosol and components of cytoskeleton with membranes. Conclusion: It was shown that distinctions between the effects of extracts on erythrocytes were mainly manifested at cellular membrane level, not affecting main vital parameters. [J Exp Integr Med 2012; 2(3.000: 213-217

  17. Assessing the impact of protein extraction methods for human gut metaproteomics.

    Science.gov (United States)

    Zhang, Xu; Li, Leyuan; Mayne, Janice; Ning, Zhibin; Stintzi, Alain; Figeys, Daniel

    2017-07-10

    Metaproteomics is a promising methodology for the functional characterizations of the gut microbiome. However, the performance of metaproteomic analysis is affected by protein extraction protocols in terms of the amount of protein recovered and the relative abundance of different bacteria observed in microbiome. Currently, there is a lack of consistency on protein extraction methods in published metaproteomics studies. Here we evaluated the effects of different protein extraction methods on human fecal metaproteome characterizations. We found that sodium dodecyl sulfate (SDS)-based lysis buffer obtained higher protein yields and peptide/protein group identifications compared to urea and the non-ionic detergent-based B-Per buffer. The addition of bead beating to any of the extraction buffers increased both protein yields and protein identifications. As well, bead beating led to a significant increase of the relative abundances of Firmicutes and Actinobacteria. We also demonstrated that ultrasonication, another commonly used mechanical disruption approach, performed even better than bead beating for gut microbial protein extractions. Importantly, proteins of the basic metabolic pathways showed significantly higher relative abundances when using ultrasonication. Overall, these results demonstrate that protein extraction protocols markedly impact the metaproteomic results and recommend a protein extraction protocol with both SDS and ultrasonication for metaproteomic studies. The gut microbiome is emerging as an important factor influencing human health. Metaproteomics is promising for advancing the understanding of the functional roles of the microbiome in disease. However, metaproteomics suffers from a lack of consistent sample preparation procedures. In the present study, protein extraction protocols for fecal microbiome samples were evaluated for their effects on protein yields, peptide identifications, protein group identifications, taxonomic compositions and

  18. EXTRACTING HUMAN BEHAVIORAL PATTERNS BY MINING GEO-SOCIAL NETWORKS

    Directory of Open Access Journals (Sweden)

    M. Forghani

    2014-10-01

    Full Text Available Accessibility of positioning technologies such as GPS offer the opportunity to store one’s travel experience and publish it on the web. Using this feature in web-based social networks and considering location information shared by users as a bridge connecting the users’ network to location information layer leads to the formation of Geo-Social Networks. The availability of large amounts of geographical and social data on these networks provides rich sources of information that can be utilized for studying human behavior through data analysis in a spatial-temporal-social context. This paper attempts to investigate the behavior of around 1150 users of Foursquare network by making use of their check-ins. The authors analyzed the metadata associated with the whereabouts of the users, with an emphasis on the type of places, to uncover patterns across different temporal and geographical scales for venue category usage. The authors found five groups of meaningful patterns that can explore region characteristics and recognize a number of major crowd behaviors that recur over time and space.

  19. 微创与传统拔牙法拔除下颌阻生第三磨牙术后愈合情况比较研究%Comparison study of atraumatic and traditional extraction for impacted mandibular third molar

    Institute of Scientific and Technical Information of China (English)

    冷颖; 邬琪; 段昌华; 莫振飞

    2013-01-01

    目的:评价微创与传统拔牙法拔除下颌阻生第三磨牙的术后愈合情况,进一步验证微创拔牙法的优越性。方法选取2010年9月至2012年10月在佛山市禅城区向阳医院·禅城区口腔医院就诊,且需要拔除中位、前倾阻生下颌第三磨牙的男性患者100例,随机分为试验组和对照组(各50例)。试验组采用微创拔牙法,对照组采用传统拔牙法。比较2组患者术后发生水肿、疼痛、张口受限等情况的差异,并记录2组患者牙槽窝骨愈合过程中的骨密度曲线图。结果试验组患者术后水肿、疼痛的发生率低于对照组(P0.05)。结论微创拔牙术有利于牙槽窝的愈合,值得临床推广应用。%Objective To research the healing of impacted mandibular third molar through atraumatic extraction so as to further verify the advantages of atraumatic extraction. Methods Totally 100 cases of male patients with median-leaning forward impacted mandibular third molar from September 2010 to October 2012 in Chancheng District Xiangyang Hospi-tal of Foshan were selected as experiment group and control group at random. Each group had 50 cases. For the experi-ment group,we adopted atraumatic extraction. For the control group,we used traditional extraction. Then we compared the differences in postoperative edema,pain and limited mouth opening of two groups,and observed two groups density curve of the alveolar bone in the healing process. Results For the experiment group,postoperative edema and pain rates were lower than the control group(P0.05). Conclusion Atraumatic ex-traction is good for the healing of tooth socket,which is worthy of clinical popularization and application.

  20. In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens

    Institute of Scientific and Technical Information of China (English)

    Ruban P; Gajalakshmi K

    2012-01-01

    Objective: To access the in vitro antibacterial activity of Hibiscus rosa-sinensis (H. rosa- sinensis) flower extract against human pathogens. Methods: Antibacterial activity was evaluated by using disc and agar diffusion methods. The protein was run through poly acrylmide gel electrophoresis to view their protein profile. Results: The results showed that the cold extraction illustrates a maximum zone of inhibition against Bacillus subtillis (B. subtillis), Escherichia coli (E. coli) viz., (17.00 ± 2.91), (14.50 ± 1.71) mm, followed by hot extraction against, E. coli, Salmonella sp. as (11.66 ± 3.14), (10.60 ± 3.09) mm. In methanol extraction showed a highest zone of inhibition recorded against B. subtillis, E. coli as (18.86 ± 0.18), (18.00 ± 1.63) mm pursued by ethanol extraction showed utmost zone of inhibition recorded against Salmonella sp. at (20.40 ± 1.54) mm. The crude protein from flower showed a maximum inhibitory zone observed against Salmonella sp., E. coliviz., (16.55 ± 1.16), (14.30 ± 2.86) mm. The flower material can be taken as an alternative source of antibacterial agent against the human pathogens. Conclusions: The extracts of the H. rosa-sinensis are proved to have potential antibacterial activity, further studies are highly need for the drug development.

  1. Withania somnifera Root Extract Has Potent Cytotoxic Effect against Human Malignant Melanoma Cells.

    Science.gov (United States)

    Halder, Babli; Singh, Shruti; Thakur, Suman S

    2015-01-01

    In Ayurveda, Withania somnifera is commonly known as Ashwagandha, its roots are specifically used in medicinal and clinical applications. It possesses numerous therapeutic actions which include anti-inflammatory, sedative, hypnotic and narcotic. Extracts from this plant have been reported for its anticancer properties. In this study we evaluated for the first time, the cytotoxic effect of Withania root extract on human malignant melanoma A375 cells. The crude extract of Withania was tested for cytotoxicity against A375 cells by MTT assay. Cell morphology of treated A375 cells was visualized through phase contrast as well as fluorescence microscopy. Agarose gel electrophoresis was used to check DNA fragmentation of the crude extract treated cells. Crude extract of Withania root has the potency to reduce viable cell count in dose as well as time dependent manner. Morphological change of the A375 cells was also observed in treated groups in comparison to untreated or vehicle treated control. Apoptotic body and nuclear blebbing were observed in DAPI stained treated cells under fluorescence microscope. A ladder of fragmented DNA was noticed in treated cells. Thus it might be said that the crude water extract of Withania somnifera has potent cytotoxic effect on human malignant melanoma A375 cells.

  2. Anti-Endometriotic Effects of Pueraria Flower Extract in Human Endometriotic Cells and Mice

    OpenAIRE

    Ji‐Hyun Kim; Jeong‐Hwa Woo; Hye Mi Kim; Myung Sook Oh; Dae Sik Jang; Jung‐Hye Choi

    2017-01-01

    Pueraria flowers have been used as a vegetable and an ingredient for tea and jelly. In this study, we investigated the effects of Pueraria flower extract (PFE) on endometriosis, a common gynaecological disease characterised by local sterile inflammation of peritoneal cavity. PFE suppressed the adhesion of human endometriotic cells 11Z and 12Z to human mesothelial Met5A cells. In addition, PFE significantly inhibited the migration of 11Z and 12Z cells as shown by woundhealing and transwel...

  3. A influência do protocolo de extração de dois pré-molares superiores na estabilidade oclusal do tratamento da Classe II Influence of the extraction protocol of two maxillary premolars on the occlusal stability of Class II treatment

    Directory of Open Access Journals (Sweden)

    Leonardo Tavares Camardella

    2010-08-01

    Full Text Available OBJETIVO: com o propósito de avaliar a influência da extração de dois pré-molares superiores na estabilidade oclusal do tratamento da má oclusão de Classe II completa, foi realizada uma comparação com o protocolo de tratamento sem extrações. MÉTODOS: selecionou-se, a partir das documentações do arquivo da Disciplina de Ortodontia da Faculdade de Odontologia de Bauru, uma amostra composta pelas documentações de 59 pacientes com má oclusão de Classe II completa. Em seguida, dividiu-se essa amostra em dois grupos, apresentando as seguintes características: Grupo 1, constituído por 29 pacientes, tratados sem extrações; e Grupo 2, composto por 30 pacientes, tratados com extrações de dois pré-molares superiores. Os modelos ao início do tratamento, ao final do tratamento e em um período mínimo de 2,4 anos após o tratamento foram medidos e avaliados por meio dos índices oclusais IPT e PAR. As condições oclusais ao final do tratamento e no estágio pós-tratamento, o percentual de recidiva e as alterações oclusais pós-tratamento foram comparados por meio do teste t. RESULTADOS: os resultados demonstraram que os protocolos de tratamento sem extração e com extrações de dois pré-molares superiores não apresentaram, em nenhuma das variáveis avaliadas, diferenças estatisticamente significativas em relação à estabilidade oclusal do tratamento da má oclusão de Classe II completa. CONCLUSÃO: a extração de dois pré-molares superiores no tratamento da má oclusão de Classe II completa não influenciou a estabilidade dos resultados oclusais alcançados ao final da correção ortodôntica. Portanto, terminar o tratamento com uma relação molar em Classe II ou em Classe I proporciona estabilidade semelhante.OBJECTIVE: With the purpose of evaluating the influence of two upper premolar extraction on the occlusal stability of full cusp Class II malocclusion treatment, a comparison was performed with a non-extraction

  4. In vitro anticancer activity of extracts of Mentha Spp. against human cancer cells.

    Science.gov (United States)

    Sharma, Vikas; Hussain, Shabir; Gupta, Moni; Saxena, Ajit Kumar

    2014-10-01

    In vitro anticancer potential of methanolic and aqueous extracts of whole plants of Mentha arvensis, M. longifolia, M. spicata and M. viridis at concentration of 100 μg/ml was evaluated against eight human cancer cell lines--A-549, COLO-205, HCT-116, MCF-7, NCI-H322, PC-3, THP-1 and U-87MG from six different origins (breast, colon, glioblastoma, lung, leukemia and prostate) using sulphorhodamine blue (SRB) assay. Methanolic extracts of above-mentioned Mentha Spp. displayed anti-proliferative effect in the range of 70-97% against four human cancer cell lines, namely COLO-205, MCF-7, NCI-H322 and THP-1; however, aqueous extracts were found to be active against HCT-116 and PC-3. The results indicate that Mentha Spp. contain certain constituents with cytotoxic properties which may find use in developing anticancer agents.

  5. Protective Effect of Strawberry Extract against Inflammatory Stress Induced in Human Dermal Fibroblasts

    Directory of Open Access Journals (Sweden)

    Massimiliano Gasparrini

    2017-01-01

    Full Text Available A protracted pro-inflammatory state is a major contributing factor in the development, progression and complication of the most common chronic pathologies. Fruit and vegetables represent the main sources of dietary antioxidants and their consumption can be considered an efficient tool to counteract inflammatory states. In this context an evaluation of the protective effects of strawberry extracts on inflammatory stress induced by E. coli LPS on human dermal fibroblast cells was performed in terms of viability assays, ROS and nitrite production and biomarkers of oxidative damage of the main biological macromolecules. The results demonstrated that strawberry extracts exerted an anti-inflammatory effect on LPS-treated cells, through an increase in cell viability, and the reduction of ROS and nitrite levels, and lipid, protein and DNA damage. This work showed for the first time the potential health benefits of strawberry extract against inflammatory and oxidative stress in LPS-treated human dermal fibroblast cells.

  6. Antimutagenic effect of aqueous extract from Agaricus brasiliensis on culture of human lymphocytes.

    Science.gov (United States)

    Gameiro, Paula H; Nascimento, José S; Rocha, Beatriz H G; Piana, Clause F B; Santos, Raquel A; Takahashi, Catarina S

    2013-02-01

    The mushroom Agaricus brasiliensis (sun mushroom), native from the southeast of Brazil, is well known by its medicinal properties that include effects on diabetes, cholesterol levels, and osteoporosis. The antimutagenic effects of A. brasiliensis has been investigated recently and revealed some controversial results depending on the temperature by which the A. brasiliensis tea is obtained. In the present study, we evaluated the effect of the A. brasiliensis extract prepared in two different temperatures, 4°C and 25°C, on the doxorubicin-induced DNA strand breaks and chromosomal aberrations (CAs) in human lymphocytes. The results demonstrated that A. brasiliensis was able to reduce the DXR-induced DNA damage in both temperatures; however, the CA test was more sensitive to demonstrate a better reduction when the cells were treated with an extract obtained at 25°C. A. brasiliensis extract obtained in different temperatures exhibited antigenotoxic and anticlastogenic effects in human lymphocytes.

  7. Water soluble nanocurcumin extracted from turmeric challenging the microflora from human oral cavity.

    Science.gov (United States)

    Gopal, Judy; Muthu, Manikandan; Chun, Se-Chul

    2016-11-15

    Water soluble nanocurcumin prepared from commercial turmeric powders was compared against ethanol extracted curcumin particles. The oral microflora from five different human volunteers was collected and the efficacy of solvent extracted curcumin versus water extracted nanocurcumin was demonstrated. Nanocurcumin activity against oral microflora confirms its antimicrobial potency. Confocal laser scanning microscopic results revealed the enhanced entry of nanocurcumin particles into microbial cells. The nanosized nature of nanocurcumin appears to have led to increased cellular interaction and thereby efficient destruction of microbial cells in the mouth. In addition, solubility of nanocurcumin is also believed to be a crucial factor behind its successful antimicrobial activity. This study proves that the bioactivity of a compound is greatly influenced by its solubility in water. This work recommends the use of water soluble nanocurcumin (extracted from turmeric) as potent substitute for curcumin in dental formulations.

  8. Hypocholesterolemic property of Yucca schidigera and Quillaja saponaria extracts in human body.

    Science.gov (United States)

    Kim, Sang-Woo; Park, Sang-Kyu; Kang, Sung-Il; Kang, Han-Chul; Oh, Han-Jin; Bae, Chul-Young; Bae, Dong-Ho

    2003-12-01

    This study was undertaken to observe the effects of the blend of partially purified Yucca schidigera and Quillaja saponaria extracts on cholesterol levels in the human's blood and gastrointestinal functions, and to determine if a new cholesterol-lowering drug can be developed by the further purification of the extracts. Ultrafiltration and sequential diafiltration increased the amounts of steroidal saponin in aqueous yucca extract and terpenoid saponin in aqueous quillaja extract from 9.3% and 21.4% to 17.2% and 61.8%, respectively. Taking 0.9 mg of the blend (6:4, v:v) of the resulting filtrates a day for 4 weeks resulted in the decreases in total and LDL cholesterol levels in blood plasma of hyper-cholesterolemic patients with enhancement in gastrointestinal symptoms of patients.

  9. The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cellss.

    Science.gov (United States)

    Chen, Lihong; Liu, Lu; Wu, Chengtie; Yang, Ruiqi; Chang, Jiang; Wei, Xi

    2017-09-02

    Biomaterials have a profound effect on tissue engineering and regenerative medicine, but few studies have reported the role of extracts from bioceramics in the regulation of stem cell pluripotency. The present study investigated the effects of bioceramics extracts, including silicate bredigite (Ca7 MgSi4 O16 ) and conventional β-tricalcium phosphate (β-TCP), on the pluripotency and the multilineage differentiation potential of human dental pulp cells (hDPCs). Basic fibroblast growth factor (bFGF), which is a known regulator of hDPCs pluripotency, was used as a reference. Bredigite extracts significantly promoted cell growth, proliferation, TERT expression and maintained hDPCs in a presenescent state. The extracts of bredigite significantly up-regulated the expression of pluripotency-related genes such as Stro1, Oct4 and Sox2, and further promoted the multilineage differentiation of hDPCs after odontogenic/adipogenic induction. The stimulation of bredigite extracts on hDPCs pluripotency was comparable to that of bFGF, whereas β-TCP extracts lacked these properties. Our results suggested for the first time that bredigite extracts enhance the pluripotency of dental-derived stem cells, paving the way for extended applications in regenerative medicine. This article is protected by copyright. All rights reserved. © 2017 Wiley Periodicals, Inc.

  10. Vinegar as a disinfectant of extracted human teeth for dental educational use

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    Manisha Tijare

    2014-01-01

    Full Text Available Context: Extracted human teeth are routinely used in dentistry to learn technical and preclinical skills. Centers for Disease Control and Prevention (CDC has adopted guidelines for infection control of extracted teeth used for research and teaching, requiring that teeth be sterilized before use. Many of the proposed disinfection methods starting from use of formalin, sodium hypochlorite and to autoclaving have their own drawbacks and may not be practical. Aim: To assess the effectiveness of vinegar for disinfection/sterilization of extracted human teeth. Materials and Methods: A total of 80 extracted non-carious human teeth were kept in seven disinfectant media-10% formalin, 3% hydrogen peroxide, 5.25% sodium hypochlorite, 70% alcohol, vinegar, neem extract and normal saline. Ten samples were placed in each disinfectant individually for a period of 7 days, at room temperature. In all, 10 teeth were treated with microwave irradiation at 650 W for 3 min. Later, teeth from each group were placed individually in separate test tubes containing 10 ml of tryptic soy broth at 37°C for 48 h to observe the evidence of growth of microorganisms. Semiquantitative analysis of all the samples was done in Clade agar at 37°C for 48 h. Statistical Analysis Used: The number of teeth disinfected in each group was compared using Chi square test. Results: 10% formalin, 3% hydrogen peroxide and vinegar were totally effective. The result was statistically significant with a Chi square value of 61.414 and P < 0.001. Conclusions: Vinegar can be used as an effective disinfectant medium for extracted human teeth.

  11. Quality of life following third molar removal under conscious sedation

    Science.gov (United States)

    Sancho-Puchades, Manuel; Berini-Aytés, Leonardo; Gay-Escoda, Cosme

    2012-01-01

    Aim: The aim of this study was to assess quality of life (QoL) and degree of satisfaction among outpatients subjected to surgical extraction of all four third molars under conscious sedation. A second objective was to describe the evolution of self-reported pain measured in a visual analogue scale (VAS) in the 7 days after extraction. Study design: Fifty patients received a questionnaire assessing social isolation, working isolation, eating and speaking ability, diet modifications, sleep impairment, changes in physical appearance, discomfort at suture removal and overall satisfaction at days 4 and 7 after surgery. Pain was recorded by patients on a 100-mm pain visual analogue scale (VAS) every day after extraction until day 7. Results: Thirty-nine patients fulfilled correctly the questionnaire. Postoperative pain values suffered small fluctuations until day 5 (range: 23 to 33 mm in a 100-mm VAS), when dicreased significantly. A positive association was observed between difficult ranked surgeries and higher postoperative pain levels. The average number of days for which the patient stopped working was 4.9. Conclusion: The removal of all third molars in a single appointment causes an important deterioration of the patient’s QoL during the first postoperative week, especially due to local pain and eating discomfort. Key words:Third molar removal, quality of life, sedation. PMID:22926461

  12. The anesthetic effects of Gow-Gates technique of inferior alveolar nerve block in impacted mandibular third molar extraction%Gow-Gates法下牙槽神经阻滞麻醉在下颌阻生第三磨牙拔除术中的麻醉效果研究

    Institute of Scientific and Technical Information of China (English)

    杨介平; 刘伟; 高庆红

    2013-01-01

    目的 通过临床随机对照试验的方法评价Gow-Gates法下牙槽神经阻滞麻醉在下颌阻生第三磨牙拔除术中的麻醉有效性和安全性.方法 使用左右半口设计,32例患者的左右下颌阻生第三磨牙分别随机采用Gow-Gates法和传统注射法进行下牙槽神经阻滞麻醉,并拔除下颌阻生第三磨牙,记录麻醉效果及不良事件.结果 所有患者均完成研究.Gow-Gates法的麻醉成功率为96.9%,传统注射法的麻醉成功率为90.6%,二者的麻醉成功率无统计学差异(P=0.317).在麻醉程度上,Gow-Gates法麻醉程度为A和B级的比率为96.9%,明显好于传统注射法的78.1%(P=0.034).Gow-Gates法的回抽出血率明显低于传统注射法(P=0.025),2种注射方法均未出现血肿.结论 Gow-Gates法下牙槽神经阻滞麻醉在下颌阻生第三磨牙拔除术中的麻醉效果好且较为安全,可以作为传统注射法的有效补充.%Objective To evaluate the anesthetic effects and safety of Gow-Gates technique of inferior alveolar nerve block in impacted mandibular third molar extraction.Methods A split-mouth study was designed.The bilateral impacted mandibular third molar of 32 participants were divided into Gow-Gates technique of inferior alveolar nerve block (Gow-Gates group) and conventional technique of inferior alveolar nerve block (conventional group) randomly with third molar extracted.The anesthetic effects and adverse events were recorded.Results All the participants completed the research.The anesthetic success rate was 96.9% in Gow-Gates group and 90.6% in conventional group with no statistical difference(P=0.317); but when comparing the anesthesia grade,Gow-Gates group had a 96.9% of grade A and B,and conventional group had a rate of 78.1%(P=0.034).And the Gow-Gates group had a much lower withdrawn bleeding than conventional group(P=0.025).Two groups had no hematoma.Conclusion Gow-Gates technique had a reliable anesthesia effects and safety in

  13. 碘纺纱条对下颌近中低位阻生牙拔除术后干槽症的预防作用%Explore The Ef ect Of Iodoform Gauze On Prevention Of Dry Socket Syndrome Caused By Low Mesial Impacted Mandibular Third Molar Extraction

    Institute of Scientific and Technical Information of China (English)

    刘瑾

    2015-01-01

    Objective:This study was to explore the ef ect of iodoform gauze on prevention of dry socket syndrome caused by low mesial impacted mandibular third molar extraction.Meth-ods:100 low mesial impacted mandibular third molars which were extracted were divided into two groups:iodoform gauze group and simple suture group.In the iodoform group ,the teeth extrac-tion sockets were fil ed with iodoform gauze based on tight suture.In the Simple suture group,the teeth extraction sockets were fil ed with nothing.Incidence of dry socket syndrome of two groups was compared.Results:In the iodoform gauze group,there is no case of dry socket(0%).However,there were 5cases of dry socket in the simple suture group(10%).The results showed the in-cidence of dry socket syndrome was significantly lower in the iodoform gauze group compared with simple suture group.The dif erence was significant .Conclusions:Iodoform gauze has a good pre-ventive action for dry socket syndrome.%目的:探讨碘纺纱条对下颌近中低位阻生牙拔出后干槽症的预防作用。方法100颗下颌近中低位阻生牙随机分为碘仿纱条组和单纯缝合组。碘仿纱条组在严密缝合的基础上用碘仿纱条填塞拔牙创,单纯缝合组拔牙创内未放置任何药物。对两组干槽症的发生率进行对比研究。结果碘仿纱条组无干槽症发生,单纯缝合组出现5例干槽症病例(10%)。碘纺纱条组干槽症的发生率明显低于单纯缝合组,差异具有显著性(P<0.05)。结论碘仿纱条对干槽症有良好的预防作用。

  14. The molecular mechanisms of Curcuma Wenyujin extract-mediated inhibitory effects on human esophageal carcinoma cells in Vitro

    Institute of Scientific and Technical Information of China (English)

    景钊

    2012-01-01

    Objective To study the molecular mechanisms of Curcuma Wenyujin extract-mediated inhibitory effects on human esophageal carcinoma cells. Methods The Curcuma Wenyujin extract was obtained by supercritical carbon dioxide extraction. TE-1 cells were divided into 4 groups after adherence.

  15. Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells

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    Xiao J.B.

    2006-01-01

    Full Text Available The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299 and liver carcinoma (HepG2 cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 µg/mL, cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. The extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. The ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and liver carcinoma cells with IC50 values of 100 and 30 µg/mL, respectively. A high concentration of ethyl acetate extract (100 µg/mL rapidly reduced the number of H1299 cells. At lower concentrations of ethyl acetate extract (15, 30, and 40 µg/mL, the numbers of HepG2 cells started to decrease markedly. Gas chromatography-mass spectrometry analysis of the ethyl acetate extract revealed the presence of several compounds such as phytol (23.42%, 1,2,4-tripropylbenzene (13.09%, 9-cedranone (12.75%, ledene oxide (7.22%, caryophyllene (1.82%, and caryophyllene oxide (1.15%. HPLC analysis result showed that there were no flavonoids in ethyl acetate extract, but flavonoids are abundant in n-butanol extract. Further studies are needed regarding the identification, toxicity, and mechanism of action of active compounds.

  16. Cytotoxic activity of kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines

    Institute of Scientific and Technical Information of China (English)

    Yu Hua Wong; Wai Yan Tan; Chin Ping Tan; Kamariah Long; Kar Lin Nyam

    2014-01-01

    Objective: To examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, human breast cancer, human colon cancer and human lung cancer cell lines.Methods:kenaf seed oil on human cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope.Results:The in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected.Conclusions:KSE and KSO could be potential sources of natural anti-cancer agents. Further The kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the investigations on using kenaf seeds for anti-proliferative properties are warranted.

  17. Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces.

    Science.gov (United States)

    Nechvatal, Jordan M; Ram, Jeffrey L; Basson, Marc D; Namprachan, Phanramphoei; Niec, Stephanie R; Badsha, Kawsar Z; Matherly, Larry H; Majumdar, Adhip P N; Kato, Ikuko

    2008-02-01

    Feces contain intestinal bacteria and exfoliated epithelial cells that may provide useful information concerning gastrointestinal tract health. Intestinal bacteria that synthesize or metabolize potential carcinogens and produce anti-tumorigenic products may have relevance to colorectal cancer, the second most common cause of cancer deaths in the USA. To facilitate epidemiological studies relating bacterial and epithelial cell DNA and RNA markers, preservative/extraction methods suitable for self-collection and shipping of fecal samples at room temperature were tested. Purification and PCR amplification of fecal DNA were compared after preservation of stool samples in RNAlater (R) or Paxgene (P), or after drying over silica gel (S) or on Whatman FTA cards (W). Comparisons were made to samples frozen in liquid nitrogen (N2). DNA purification methods included Whatman (accompanying FTA cards), Mo-Bio Fecal (MB), Qiagen Stool (QS), and others. Extraction methods were compared for amount of DNA extracted, DNA amplifiable in a real-time SYBR-Green quantitative PCR format, and the presence of PCR inhibitors. DNA can be extracted after room temperature storage for five days from W, R, S and P, and from N2 frozen samples. High amounts of total DNA and PCR-amplifiable Bacteroides spp. DNA (34%+/-9% of total DNA) with relatively little PCR inhibition were especially obtained with QS extraction applied to R preserved samples (method QS-R). DNA for human reduced folate carrier (SLC19A1) genomic sequence was also detected in 90% of the QS-R extracts. Thus, fecal DNA is well preserved by methods suitable for self-collection that may be useful in future molecular epidemiological studies of intestinal bacteria and human cancer markers.

  18. Differential Effects of Tea Extracts on Growth and Cytokine Production by Normal and Leukemic Human Leukocytes

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    Diana Bayer

    2012-04-01

    Full Text Available Background: Tea is one of the world’s most highly consumed beverages, second only to water. It is affordable and abundant and thus has great potential for improving health of those in both developed and developing areas. Green, oolong, and black teas differ in the extent of fermentation and types of bioactive polyphenols produced. Green tea and its major polyphenol decrease growth of some cancer cells and effect production of immune system cytokines. This study compares the effects of different types of tea extracts on viability and cytokine production by normal and leukemic human T lymphocytes. Generation of the toxic reactive oxygen species H2O2 by extracts was also examined.Methods: The Jurkat T lymphoblastic leukemia cells and mitogen-stimulated normal human peripheral blood mononuclear cells were used in this study. Cell viability was determined by (3-4,5-dimethylthiamizol-2-yl-diphenyltetrazolium bromide assay and production of interleukin-2 by Enzyme-Linked ImmunoSorbent Assay. Levels of H2O2 generated by tea extracts were determined using the xylenol-orange method.Results: We found that green, oolong, and black tea extracts differentially effect the growth and viability of T lymphoblastic leukemia cells and normal peripheral blood mononuclear cells, substantially decreasing both growth and viability of leukemic T lymphocytes and having much lesser effects on their normal counterparts. Tea extracts also had differential effects on the production of the T lymphocyte growth factor interleukin-2, significantly decreasing production by leukemic cells while having only minor effects on normal cells. All three extracts induced H2O2 generation, with green and oolong tea extracts having the greatest effect. Leukemic cells were much more susceptible to growth inhibition and killing by H2O2 than normal lymphocytes.Functional Foods in Health and Disease 2012, 2(4:72-85 Conclusions: The three tea extracts studied altered leukemic T lymphocyte

  19. A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma.

    Science.gov (United States)

    McDonald, Jeffrey G; Smith, Daniel D; Stiles, Ashlee R; Russell, David W

    2012-07-01

    We describe the development of a method for the extraction and analysis of 62 sterols, oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-MS. Deuterated standards are added to 200 μl of human plasma. Bulk lipids are extracted with methanol:dichloromethane, the sample is hydrolyzed using a novel procedure, and sterols and secosteroids are isolated using solid-phase extraction (SPE). Compounds are resolved on C₁₈ core-shell HPLC columns and by GC. Sterols and oxysterols are measured using triple quadrupole mass spectrometers, and lathosterol is measured using GC-MS. Detection for each compound measured by HPLC-MS was ∪ 1 ng/ml of plasma. Extraction efficiency was between 85 and 110%; day-to-day variability showed a relative standard error of <10%. Numerous oxysterols were detected, including the side chain oxysterols 22-, 24-, 25-, and 27-hydroxycholesterol, as well as ring-structure oxysterols 7α- and 4β-hydroxycholesterol. Intermediates from the cholesterol biosynthetic pathway were also detected, including zymosterol, desmosterol, and lanosterol. This method also allowed the quantification of six secosteroids, including the 25-hydroxylated species of vitamins D₂ and D₃. Application of this method to plasma samples revealed that at least 50 samples could be extracted in a routine day.

  20. Antioxidant and genoprotective effects of spent coffee extracts in human cells.

    Science.gov (United States)

    Bravo, Jimena; Arbillaga, Leire; de Peña, M Paz; Cid, Concepcion

    2013-10-01

    Spent coffee has been shown as a good source of hydrophilic antioxidant compounds. The ability of two spent coffee extracts rich in caffeoylquinic acids, mainly dicaffeoylquinic acids, and caffeine (Arabica filter and Robusta espresso) to protect against oxidation and DNA damage in human cells (HeLa) was evaluated at short (2 h) and long (24 h) exposure times. Cell viability (MTT) was not affected by spent coffee extracts (>80%) up to 1000 μg/mL after 2 h. Both spent coffee extracts significantly reduced the increase of ROS level and DNA strand breaks (29-73% protection by comet assay) induced by H₂O₂. Pretreatment of cells with robusta spent coffee extract also decreased Ro photosensitizer-induced oxidative DNA damage after 24 h exposure. The higher effectiveness of Robusta spent coffee extract, with less caffeoylquinic acids and melanoidins, might be due to other antioxidant compounds, such as caffeine and other Maillard reaction products. This work evidences the potential antioxidant and genoprotective properties of spent coffee in human cells. Copyright © 2013 Elsevier Ltd. All rights reserved.

  1. Estudo cefalométrico das alterações das alturas faciais anterior e posterior em pacientes leucodermas, com má oclusão de classe II, 1ª divisão de Angle, tratados com e sem extração de quatro primeiros pré-molares The influence of orthodontic extraction and nonextraction treatment on anterior and posterior facial heights

    Directory of Open Access Journals (Sweden)

    Daniele Teixeira Machado

    2005-12-01

    Full Text Available OBJETIVO: este estudo cefalométrico longitudinal visou avaliar, comparativamente, as alterações das alturas faciais, provenientes do tratamento da má oclusão de Classe II, 1ª divisão de Angle, pela Técnica de "Edgewise", associado à ancoragem extrabucal cervical, com e sem extração de quatro primeiros pré-molares. METODOLOGIA: a amostra constituiu-se de 116 telerradiografias em norma lateral obtidas de 56 jovens, de ambos os gêneros. Destes jovens, 22 foram tratados ortodonticamente com extração de quatro primeiros pré-molares e apresentavam idade média inicial de 12,30 e final de 14,87 anos, constituindo o grupo I. Outros 22 foram tratados ortodonticamente sem extração e apresentavam idade média inicial de 12,53 e idade média final de 14,73 anos, formando o grupo II. Os demais 14 jovens com idade média inicial de 11,50 e final de 13,63 anos, não foram submetidos a nenhuma intervenção ortodôntica, formando assim o grupo controle (grupo III. RESULTADOS E CONCLUSÕES: os resultados demonstraram que não houve influência significante da terapia ortodôntica sobre as alterações das alturas faciais. Os primeiros molares superiores e inferiores desenvolveram-se no sentido vertical nos três grupos. As alterações horizontais dentoalveolares evidenciaram uma mesialização dos primeiros molares superiores e inferiores em todos os grupos, contudo o maior deslocamento para mesial ocorreu no grupo tratado com extração de quatro primeiros pré-molares e no grupo controle. Este fato prova que, no grupo tratado sem extração, a Classe II foi corrigida não pela distalização dos primeiros molares superiores, mas pela restrição da mesialização dos mesmos.AIM: the cephalometric changes of anterior and posterior facial heights, in the Class II, Division 1 treatment, were compared among three different groups of patients: 4-premolar extraction, non-extraction and control. METHODS: each test sample consisted of 22 patients

  2. Molar incisor hypomineralization: review and recommendations for clinical management.

    Science.gov (United States)

    William, Vanessa; Messer, Louise B; Burrow, Michael F

    2006-01-01

    Molar incisor hypomineralization (MIH) describes the clinical picture of hypomineralization of systemic origin affecting one or more first permanent molars (FPMs) that are associated frequently with affected incisors. Etiological associations with systemic conditions or environmental insults during the child's first 3 years have been implicated. The complex care involved in treating affected children must address their behavior and anxiety, aiming to provide a durable restoration under pain-free conditions. The challenges include adequate anaesthesia, suitable cavity design, and choice of restorative materials. Restorations in hypomineralized molars appear to fail frequently; there is little evidence-based literature to facilitate clinical decisions on cavity design and material choice. A 6-step approach to management is described: (1) risk identification; (2) early diagnosis; (3) remineralization and desensitization; (4) prevention of caries and posteruption breakdown; (5) restorations and extractions; and (6) maintenance. The high prevalence of MIH indicates the need for research to clarify etiological factors and improve the durability of restorations in affected teeth. The purpose of this paper was to describe the diagnosis, prevalence, putative etiological factors, and features of hypomineralized enamel in molar incisor hypomineralization and to present a sequential approach to management.

  3. Toxicological and melanin synthesis effects of Polygonum multiflorum root extracts on zebrafish embryos and human melanocytes

    Directory of Open Access Journals (Sweden)

    Thanh Thi Hoai Dang

    2016-09-01

    Full Text Available Polygonum multiflorum (PM has been commmonly used as folk medicine for treatment of various conditions, such as early graying of hair in humans. However, there have been limited studies which have evaluated the toxicological and biological effects of PM in vitro as well as in vivo. In this study, PM root extracts in ethyl acetate (PM-E and in distilled water (PM-W were examined for their effects on the development of teratogenic defects/deaths. Additionally, they were evaluated for their effects on melanin formation in human melanocytes and pigmentation in embryos/larvae of wild type strain AB zebrafish (Danio rerio. Our results showed that PM root extracts at concentrations of 40 mg/L and 105 mg/L induced the development of teratogenic defects, including yolk sac edema (or heart edema, hemovascular defects, necrosis and abnormal trunk in zebrafish embryos at 4 days post fertilization; teratogenic indexes (TIs were 1.43 and 0.63 for ethyl acetate extract and distilled water extract, respectively. Our results also demonstrated that PM-W significantly increased the pigmentation level of embryos/larvae and induced melanin formation in human melanocytes. The amount of melanin in PM-W-exposed embryos/larvae was 2.2-fold and 1.71-fold greater than those in the control embryos/larvae and control melanocytes, respectively. Our study also showed that the increased level of pigmentation in PM-W embryos/larvae or melanin biosynthesis in melanocytes were both regulated by activation of tyrosinase. Conclusively, our study suggests that PM root extracts could be used as potential agents for treatment of early hair graying as well as various other diseases related to loss of pigmentation. However, these PM root extracts may also have some negative effects on embryos; therefore it should be careful when using for women during pregnancy. [Biomed Res Ther 2016; 3(9.000: 808-818

  4. An improved high-throughput lipid extraction method for the analysis of human brain lipids.

    Science.gov (United States)

    Abbott, Sarah K; Jenner, Andrew M; Mitchell, Todd W; Brown, Simon H J; Halliday, Glenda M; Garner, Brett

    2013-03-01

    We have developed a protocol suitable for high-throughput lipidomic analysis of human brain samples. The traditional Folch extraction (using chloroform and glass-glass homogenization) was compared to a high-throughput method combining methyl-tert-butyl ether (MTBE) extraction with mechanical homogenization utilizing ceramic beads. This high-throughput method significantly reduced sample handling time and increased efficiency compared to glass-glass homogenizing. Furthermore, replacing chloroform with MTBE is safer (less carcinogenic/toxic), with lipids dissolving in the upper phase, allowing for easier pipetting and the potential for automation (i.e., robotics). Both methods were applied to the analysis of human occipital cortex. Lipid species (including ceramides, sphingomyelins, choline glycerophospholipids, ethanolamine glycerophospholipids and phosphatidylserines) were analyzed via electrospray ionization mass spectrometry and sterol species were analyzed using gas chromatography mass spectrometry. No differences in lipid species composition were evident when the lipid extraction protocols were compared, indicating that MTBE extraction with mechanical bead homogenization provides an improved method for the lipidomic profiling of human brain tissue.

  5. Evaluation of methods for the extraction and purification of DNA from the human microbiome.

    Directory of Open Access Journals (Sweden)

    Sanqing Yuan

    Full Text Available BACKGROUND: DNA extraction is an essential step in all cultivation-independent approaches to characterize microbial diversity, including that associated with the human body. A fundamental challenge in using these approaches has been to isolate DNA that is representative of the microbial community sampled. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we statistically evaluated six commonly used DNA extraction procedures using eleven human-associated bacterial species and a mock community that contained equal numbers of those eleven species. These methods were compared on the basis of DNA yield, DNA shearing, reproducibility, and most importantly representation of microbial diversity. The analysis of 16S rRNA gene sequences from a mock community showed that the observed species abundances were significantly different from the expected species abundances for all six DNA extraction methods used. CONCLUSIONS/SIGNIFICANCE: Protocols that included bead beating and/or mutanolysin produced significantly better bacterial community structure representation than methods without both of them. The reproducibility of all six methods was similar, and results from different experimenters and different times were in good agreement. Based on the evaluations done it appears that DNA extraction procedures for bacterial community analysis of human associated samples should include bead beating and/or mutanolysin to effectively lyse cells.

  6. Simultaneous transdermal extraction of glucose and lactate from human subjects by reverse iontophoresis

    Directory of Open Access Journals (Sweden)

    Tak S Ching

    2008-06-01

    Full Text Available Tak S Ching1, Patricia Connolly21Asia University, Taiwan; 2Bioengineering Unit, University of Strathclyde, Glasgow, Scotland, UKAbstract: This study investigated the possibility of simultaneously extracting glucose and lactate from human subjects, at the same skin location, using transdermal reverse iontophoresis. Transdermal monitoring using iontophoresis is made possible by the skin’s permeability to small molecules and the nanoporous and microporous nature of the structure of skin. The study was intended to provide information which could be used to develop a full, biosensor-based, monitoring system for multiple parameters from transdermal extraction. As a precursor to the human study, in vitro reverse iontophoresis experiments were performed in an artificial skin system to establish the optimum current waveforms to be applied during iontophoresis. In the human study, a bipolar DC current waveform (with reversal of the electrode current direction every 15 minutes was applied to ten healthy volunteers via skin electrodes and utilized for simultaneous glucose and lactate transdermal extraction at an applied current density of 300 µA/cm2. Glucose and lactate were successfully extracted through each subject’s skin into the conducting gel that formed part of each iontophoresis electrode. The results suggest that it will be possible to noninvasively and simultaneously monitor glucose and lactate levels in patients using this approach and this could have future applications in diagnostic monitoring for a variety of medical conditions.Keywords: transdermal, iontophoresis, glucose, lactate, diagnostic monitoring

  7. Simultaneous transdermal extraction of glucose and lactate from human subjects by reverse iontophoresis.

    Science.gov (United States)

    Ching, Tak S; Connolly, Patricia

    2008-01-01

    This study investigated the possibility of simultaneously extracting glucose and lactate from human subjects, at the same skin location, using transdermal reverse iontophoresis. Transdermal monitoring using iontophoresis is made possible by the skin's permeability to small molecules and the nanoporous and microporous nature of the structure of skin. The study was intended to provide information which could be used to develop a full, biosensor-based, monitoring system for multiple parameters from transdermal extraction. As a precursor to the human study, in vitro reverse iontophoresis experiments were performed in an artificial skin system to establish the optimum current waveforms to be applied during iontophoresis. In the human study, a bipolar DC current waveform (with reversal of the electrode current direction every 15 minutes) was applied to ten healthy volunteers via skin electrodes and utilized for simultaneous glucose and lactate transdermal extraction at an applied current density of 300 microA/cm2. Glucose and lactate were successfully extracted through each subject's skin into the conducting gel that formed part of each iontophoresis electrode. The results suggest that it will be possible to noninvasively and simultaneously monitor glucose and lactate levels in patients using this approach and this could have future applications in diagnostic monitoring for a variety of medical conditions.

  8. Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells

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    B Gordanian

    2013-07-01

    Full Text Available Abstract Background & aim: Several studies have reported anti-cancer properties of sagebrush plain. The aim of this study was to evaluate the cytotoxicity of the methanol extract of sagebrush plain on human breast cancer MCF7 cells. Methods: In the present experimental study, the toxic effects of methanol extracts of flowers, leaves, stems and roots of sagebrush plain from of Khorassan and Esfahan province were tested on human breast cancer cells MCF-7 and normal cells HEK293 . Plant samples were extracted by methanol and their toxic effects on normal and breast cancer cells at concentrations of 5.62, 125, 250 and 500 µg/ml was determined by MTT. Both breast cancer cells MCF-7 and normal HEK293 cells were cultured in RPMI-1640 medium and DMEM containing 10% fetal calf serums were cultured. Data were analyzed by one-way ANOVA. Results: The methanol extract of sagebrush showed toxicity on MCF7 cells. The extract of Khorasan showed higher toxicity than Esfahan province. IC50 of sagebrush plant for all parts of the plant were obtained more than 500 µg/ml, but the IC50 of sagebrush plant of Khorasan region in leaf and flower were 205 ± 1.3 and 213 ± 5.3µg respectively. The leaves and flowers in both cases had the highest cytotoxicity. Plant extracts in both regions did not show significant cytotoxicity on normal HEK293 cells. Conclusion: The extract of the sagebrush plain region of Khorasan region showed greater cytotoxicity than Esfahan. It seems that different environmental conditionshas considerable cytotoxicity. Keywords: Sagebrush Plain, MTT, Breast Cancer

  9. Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens

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    D.A Mahmoud

    2011-09-01

    Full Text Available This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of some human pathogens (Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Candida albicans and Microsporum gypseum in vitro. Different concentrations (5, 10, 15 and 20% prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. The 20% ethyl acetate extract gave the strongest inhibition compared with the activity obtained by the same concentration of the other extracts. High Performance Liquid Chromatography (HPLC analysis of ethyl acetate extract showed the presence of a main component (nimonol which was purified and chemically confirmed by Nuclear Magnetic Resonance (NMR spectroscopic analysis. The 20% ethyl acetate extract lost a part of its antifungal effect after pooling out the nimonol and this loss in activity was variable on test pathogens. The purified nimonol as a separate compound did not show any antifungal activity when assayed against all the six fungal pathogens.

  10. Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens

    Science.gov (United States)

    Mahmoud, D.A.; Hassanein, N.M.; Youssef, K.A.; Abou Zeid, M.A.

    2011-01-01

    This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of some human pathogens (Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Candida albicans and Microsporum gypseum) in vitro. Different concentrations (5, 10, 15 and 20%) prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. The 20% ethyl acetate extract gave the strongest inhibition compared with the activity obtained by the same concentration of the other extracts. High Performance Liquid Chromatography (HPLC) analysis of ethyl acetate extract showed the presence of a main component (nimonol) which was purified and chemically confirmed by Nuclear Magnetic Resonance (NMR) spectroscopic analysis. The 20% ethyl acetate extract lost a part of its antifungal effect after pooling out the nimonol and this loss in activity was variable on test pathogens. The purified nimonol as a separate compound did not show any antifungal activity when assayed against all the six fungal pathogens. PMID:24031718

  11. Determination of triamterene in human plasma and urine after its cloud point extraction

    Directory of Open Access Journals (Sweden)

    Ahad Bavili Tabrizi

    2014-01-01

    Full Text Available A new analytical approach was developed involving cloud point extraction (CPE and spectrofluorimetric determination of triamterene (TM in biological fluids. A urine or plasma sample was prepared and adjusted to pH 7, then TM was quickly extracted using CPE, using 0.05% (w/v of Triton X-114 as the extractant. The main factors that affected the extraction efficiency (the pH of the sample, the Triton X-114 concentration, the addition of salt, the extraction time and temperature, and the centrifugation time and speed were studied and optimized. The method gave calibration curves for TM with good linearities and correlation coefficients (r higher than 0.99. The method showed good precision and accuracy, with intra- and inter-assay precisions of less than 8.50% at all concentrations. Standard addition recovery tests were carried out, and the recoveries ranged from 94.7% to 114%. The limits of detection and quantification were 3.90 and 11.7 µg L-1, respectively, for urine and 5.80 and 18.0 µg L-1, respectively, for plasma. The newly developed, environmentally friendly method was successfully used to extract and determine TM in human urine samples.

  12. Molecular events underlying maggot extract promoted rat in vivo and human in vitro skin wound healing.

    Science.gov (United States)

    Li, Pei-Nan; Li, Hong; Zhong, Li-Xia; Sun, Yuan; Yu, Li-Jun; Wu, Mo-Li; Zhang, Lin-Lin; Kong, Qing-You; Wang, Shou-Yu; Lv, De-Cheng

    2015-01-01

    Maggot extracts promote wound healing, but their bioactive part(s) and molecular effects on the regenerating tissues/cells remain largely unclear. These issues are addressed here by treating rat skin wounds, human keratinocyte line/HaCat and fibroblasts with maggot secretion/excretion, and the extracts of maggots without and with secretion/excretion. The wound closure rates, cell proliferation activities, and statuses of wound healing-related signaling pathways (STAT3, Notch1, Wnt2, NF-κB, and TGF-beta/Smad3) and their downstream gene expression (c-Myc, cyclin D1, and VEGF) are evaluated by multiple approaches. The results reveal that the maggot extracts, especially the one from the maggots without secretion/excretion, show the best wound healing-promoting effects in terms of quicker wound closure rates and more rapid growth of keratinocytes and fibroblasts. Of the five signaling pathways checked, the ones mediated by TGF-beta/Smad3, and STAT3 are activated in the untreated wounds and become further enhanced by the maggot extracts, accompanied with c-Myc, VEGF, and cyclin D1 up-regulation. Our results thus show (1) that both body extract and secretion/excretion of maggots contain favorable wound healing elements and (2) that the enhancement of TGF-beta/Smad3 and STAT3 signaling activities may be the main molecular effects of maggot extracts on the wound tissues.

  13. A salting out and resin procedure for extracting Schistosoma mansoni DNA from human urine samples

    Directory of Open Access Journals (Sweden)

    Rodrigues Nilton B

    2010-04-01

    Full Text Available Abstract Background In this paper a simple and cheap salting out and resin (InstaGene matrix® resin - BioRad DNA extraction method from urine for PCR assays is introduced. The DNA of the fluke Schistosoma mansoni was chosen as the target since schistosomiasis lacks a suitable diagnostic tool which is sensitive enough to detect low worm burden. It is well known that the PCR technique provides high sensitivity and specificity in detecting parasite DNA. Therefore it is of paramount importance to take advantage of its excellent performance by providing a simple to handle and reliable DNA extraction procedure, which permits the diagnosis of the disease in easily obtainable urine samples. Findings The description of the extraction procedure is given. This extraction procedure was tested for reproducibility and efficiency in artificially contaminated human urine samples. The reproducibility reached 100%, showing positive results in 5 assay repetitions of 5 tested samples each containing 20 ng DNA/5 ml. The efficiency of the extraction procedure was also evaluated in a serial dilution of the original 20 ng DNA/5 ml sample. Detectable DNA was extracted when it was at a concentration of 1.28 pg DNA/mL, revealing the high efficiency of this procedure. Conclusions This methodology represents a promising tool for schistosomiasis diagnosis utilizing a bio-molecular technique in urine samples which is now ready to be tested under field conditions and may be applicable to the diagnosis of other parasitic diseases.

  14. Parallel artificial liquid membrane extraction as an efficient tool for removal of phospholipids from human plasma

    DEFF Research Database (Denmark)

    Ask, Kristine Skoglund; Bardakci, Turgay; Parmer, Marthe Petrine

    2016-01-01

    Generic Parallel Artificial Liquid Membrane Extraction (PALME) methods for non-polar basic and non-polar acidic drugs from human plasma were investigated with respect to phospholipid removal. In both cases, extractions in 96-well format were performed from plasma (125μL), through 4μL organic solv...... matrix effects were investigated with fluoxetine, fluvoxamine, and quetiapine as model analytes. No signs of matrix effects were observed. Finally, PALME was evaluated for the aforementioned drug substances, and data were in accordance with European Medicines Agency (EMA) guidelines....

  15. Lectins in extracts of certain Polygonaceae seed precipitate animal and human serums.

    Science.gov (United States)

    Hanan, E B; Spindler, J W

    1968-06-28

    Seeds of four species of Polygonaceae were tested for lectins that precipitate human and animal serums. Rumex crispus, Polygonum convolvulus, and Polygonum pennsylvanicum developed specific precipitate bands on double diffusion on agar gel plates. These bands were enhanced and increased in number when extracts were tested against serums from patients with certain diseases. When tested against lyophilized serum, no precipitate bands developed. The active substance cannot be dialyzed through cellulose membrane against running tap water for 16 hours, and it is heat stable. Extracts from Fagopyrum esculentum developed no precipitate bands.

  16. 高速涡轮气钻法与凿骨劈冠法在拔除下颌水平埋伏阻生智齿中的对比研究%Comparison of High-speed Air Turbine and Traditional Chisel Technique on Extracting Impacted Mandible Third Molars

    Institute of Scientific and Technical Information of China (English)

    林静; 卢保全; 陈永锋; 王英

    2013-01-01

    Objective:To compare the clinical effects of high -speed air turbine drill and traditional chisel on removal of impacted mandible third molars .Methods:The clinical datas of the patients with 120 lower impacted mandible third molar were retrospectively analyzed .In witch high-speed air drill group(60) and chisel group(60)to be performed tooth extraction by high -speed air drill and traditional chisel osteotomy respectively .Then the duration of extraction and the intraoperative and postoperative complications were observed and recorded to assess the two methods .Results:The duration of extraction of drill group was shorter than chisel extraction group , which had statisti-cal differences between the two groups (P<0.05).The drill group had less postoperative complication such as ache , swelling, limited opening of mouth than those of the chisel extraction group (P<0.05).Occurrence of the inferior alveolar nerve injury , mandibular fracture , adjacent tooth damage and dry sock were lower than chisel extraction group (P<0.05).Conclusions:High-speed air tutbine drill with its evident superiority to traditional chisel , when applied in the extraction of low impacted teeth , can efficiently cut down duration of extraction , reduce the incidence of the intraoperative and postoperative complication and is therefore recommendable in clinical practice .%目的:对比高速涡轮气钻法与凿骨劈冠法拔除下颌水平埋伏阻生智齿的临床疗效。方法:回顾分析口腔门诊120颗低位水平阻生智齿患者的临床资料,其中采用传统的凿骨劈冠法拔除60颗为锤凿组,采用高速涡轮气钻拔除60颗为涡轮钻组,对2组手术时间及术中、术后并发症发生情况进行观察和对比分析。结果:涡轮钻组拔牙时间短于锤凿组,差异有统计学意义( P<0.05)。涡轮钻组术中邻牙损伤、下齿槽神经损伤和下颌角骨折的发生率以及术后颊面部的肿胀、疼痛程度、张口

  17. Management of Chronic Hyperplastic Pulpitis in Mandibular Molars of Middle Aged Adults- A Multidisciplinary Approach

    Science.gov (United States)

    Lingeswaran, Somiya; Ari, Geetha; Thyagarajan, Ramakrishnan; Logaranjani, Anitha

    2016-01-01

    The molar tooth of children and young adults is a common site for chronic hyperplastic pulpitis (pulp polyp). It rarely occurs in middle aged adults. This condition is usually characterized by extensive involvement of the pulp, dictating the extraction of involved tooth. Extraction of permanent molars can lead to transient or permanent malocclusion, aesthetic, phonetic and functional problems. Here we report a case of pulp polyp in mandibular first molar of a 33-year-old woman that grew into the carious cavity. The aim of this case report is to describe the diagnosis of a chronic hyperplastic pulpitis involving the permanent molar as well as to describe its management in order to preserve them as a functional unit of the dentition. PMID:26894192

  18. Effects of ozone therapy on pain, swelling, and trismus following third molar surgery.

    Science.gov (United States)

    Kazancioglu, H O; Kurklu, E; Ezirganli, S

    2014-05-01

    The aim of this study was to evaluate the efficacy of therapeutic ozone application in the management of pain, swelling, and trismus associated with the surgical removal of impacted mandibular third molars. Sixty consecutive patients with asymptomatic bilateral impacted mandibular third molars were recruited into the study. Randomly, by use of envelops, the molar on one side was extracted and ozone therapy was given (study side); the molar on the other side was extracted 2 weeks later and sham ozone therapy was given (negative control side). The mean age of the 60 patients was 22.6±2.3 years (range 18-25 years). No differences were found between the two sides for mouth opening or swelling. The degree of pain and the number of analgesic tablets taken was significantly lower for the study side. This study showed ozone therapy to have a positive effect on OHIP-14 questionnaire results.

  19. Accidental Displacement of Third Molar into the Sublingual Space: a Case Report

    Directory of Open Access Journals (Sweden)

    Rubens Jorge

    2014-10-01

    Full Text Available Background: Successful extraction of third molars depends on preoperative diagnosis and planning. Gold standard preoperative examinations are performed through computed tomography, decreasing risks and avoiding potential accidents. The present report highlights the value of preoperative examinations in face of accidentally displaced third molars. Methods: An 18-years-old female patient underwent a third mandibular molar extraction with a general dentist. Accidentally, the mandibular left third molar was displaced into the sublingual space, making necessary a second surgical step. The surgery was interrupted and the patient was referred to an expert in maxillofacial surgery. Results: After 21 days awaiting an asymptomatic health status, the second surgical step was successfully performed using multislice computed tomography as preoperative imaging guide. Conclusions: The present case report highlights the clinical usefulness of imaging planning and informed consents in face of legal and ethic potential complaints.

  20. Recombinant human proinsulin from transgenic corn endosperm: solvent screening and extraction studies

    Directory of Open Access Journals (Sweden)

    C. S. Farinas

    2007-09-01

    Full Text Available Recombinant pharmaceutical proteins are being produced in different systems such as bacteria and mammalian cell cultures. The use of transgenic plants as bioreactors has recently arisen as an alternative system offering many practical and economic advantages. However, finding an optimum strategy for the downstream processing (DSP of recombinant proteins from plants still remains a challenge. In this work, we studied the extraction of recombinant human proinsulin (rhProinsulin produced in the endosperm of transgenic corn seeds. An efficient extraction solvent was selected and the effects of temperature, solvent-to-solid ratio, time, and impeller rotational speed on the extraction were evaluated using an experimental design. After an extraction kinetics study, temperature was further evaluated to maximize rhProinsulin concentration in the extracts and to minimize the native corn components carbohydrates, phenolic compounds, and proteins. A high efficiency condition for extracting rhProinsulin with the selected solvent - 50 mM sodium bicarbonate buffer pH 10.0 and 5 mM DTT - was an extraction time of 2 h at a solvent-to-solid ratio of 10:1 and 25º C. The maximum rhProinsulin concentration in the extracts at that condition was 18.87 mg l-1 or 0.42% of the total soluble protein. These values are within the range in which the production of pharmaceutical proteins in plants can be competitive with other expression systems. The results presented provide information for the development of an additional production platform for the hormone insulin.

  1. Parallel artificial liquid membrane extraction of acidic drugs from human plasma.

    Science.gov (United States)

    Roldán-Pijuán, Mercedes; Pedersen-Bjergaard, Stig; Gjelstad, Astrid

    2015-04-01

    The new sample preparation concept "Parallel artificial liquid membrane extraction (PALME)" was evaluated for extraction of the acidic drugs ketoprofen, fenoprofen, diclofenac, flurbiprofen, ibuprofen, and gemfibrozil from human plasma samples. Plasma samples (250 μL) were loaded into individual wells in a 96-well donor plate and diluted with HCl to protonate the acidic drugs. The acidic drugs were extracted as protonated species from the individual plasma samples, through corresponding artificial liquid membranes each comprising 2 μL of dihexyl ether, and into corresponding acceptor solutions each comprising 50 μL of 25 mM ammonia solution (pH 10). The liquid membranes and the acceptor solutions were located in a 96-well filter plate, which was sandwiched with the 96-well donor plate during extraction. Parallel extraction of several samples was performed for 15 to 60 min, followed by high-performance liquid chromatography-ultraviolet detection of the individual acceptor solutions. Important PALME parameters including the chemical composition of the liquid membrane, extraction time, and sample pH were optimized, and the extraction performance was evaluated. Except for flurbiprofen, exhaustive extraction was accomplished from plasma. Linearity was obtained for all six drugs in the range 0.025-10 μg/mL, with r (2) values ranging between 0.998 and 1.000. Precision data were in the range 3-22% RSD, and accuracy data were within 72-130% with spiked plasma samples. Based on the current experiences, PALME showed substantial potential for future high-throughput bioanalysis of non-polar acidic drugs.

  2. 下颌第三磨牙拔除时下牙槽动脉出血的风险评估及急救处理%Risk assessment and first aid treatment for bleeding of inferior alveolar artery during extraction of mandibular third molar

    Institute of Scientific and Technical Information of China (English)

    兰雪松; 韩泽民

    2015-01-01

    Obsjective To assess the risk of and first aid treatment for bleeding of inferior alveolar artery during extraction ofmandibular third molar surgery through panoramic radiographic findings,and summarize bleeding. Methods Three hundred and thirty cases with impacted mandibular third molars underwent pantomography to analyze the relationship between tooth root and the inferior alveolar canal (IAC). The cases were divided into 4 types using the IAC as a reference and the bleeding cases were recorded during the third molar surgry. Results Out of the 330 cases 20 suffered from bleeding of inferior alveolar artery during surgery(20/330, 6.1%). The bleeding rate of the type 1 group was 3.0%(5/167)and the bleedinbg rate of the type 2 group was 5.7%( 6/105), both significantly lower than that of the type 3 group ,and 9 cases in the group of type 3(9/54).No bleeding cases was found in the group of type 4.Statiscal analysis showed there was significant differenc between type 1 and type 3 and type 2 and type 3(χ2 test,(P0.05).Conclusion At panoramic radiograph,the nearer the tooth root of mandibular third molars is to the IAC,the more easily inferior alveolar artery bleed during mandibular third molar surgery.It is important to analyse the adiographic signs of patients with impacted mandibular third molars before extraction and take preparation for bleeding of of inferior alveolar artery during mandibular third molar surgery.%目的:通过术前曲面体层片检查,评估下颌第三磨牙拔除术中发生下牙槽动脉出血的几率,概述出血发生时的急救处理。方法收集330例术前行曲面体层片检查的下颌阻生第三磨牙拔除患者,通过曲面体层片分析牙根与下颌管的位置关系,以下颌管为参照,将阻生牙分为4类,统计各类术中发生下牙槽动脉出血的例数,应用SPSS 20.0软件包对数据进行χ2检验,分析易导致下牙槽动脉出血的阻生类型。结果共有20例发生下牙槽

  3. Sex assessment by molar odontometrics in North Indian population

    Directory of Open Access Journals (Sweden)

    Ramandeep Singh Narang

    2015-01-01

    Full Text Available Introduction: Human identification is based on scientific principles, mainly involving dental records, fingerprints, estimation of age, postmortem reports, differentiation by blood groups, and DNA comparisons. Sex assessment is one of the prime factors employed to assist with the identification of an individual. Aims and Objective: To investigate univariate sex differences in the dimensions of permanent first molars and to assess sex, based on buccolingual (BL and mesiodistal (MD dimensions of permanent first molars in a population of north India. In addition, the study intended to evaluate the reliability of dimensional variation of these teeth in assessment of sex among the population. Materials and Methods: The study sample consists of 410 adult individuals (200 males and 210 females, from a north Indian population. The BL and MD diameters of the permanent first molars were measured using digital vernier callipers. Results: It was observed statistically significant difference between males and females with P < 0.05, in maxillary casts in both BL and MD dimensions; but only in the MD dimension in mandibular casts. A high level of sexual dimorphism of 7.7% was found in the BL dimension of the maxillary right first molar. The accuracy of sex assessment by each dimension was deliberated by univariate analyses with an overall accuracy ranging from 67.5 to 88% for various dimensions. Conclusion: Sexual dimorphism of teeth is population specific and among north Indian population, BL and MD dimensions in maxillary first molar and MD dimension in mandibular first molar can be used for sex assessment.

  4. Automatic Extraction of Destinations, Origins and Route Parts from Human Generated Route Directions

    Science.gov (United States)

    Zhang, Xiao; Mitra, Prasenjit; Klippel, Alexander; Maceachren, Alan

    Researchers from the cognitive and spatial sciences are studying text descriptions of movement patterns in order to examine how humans communicate and understand spatial information. In particular, route directions offer a rich source of information on how cognitive systems conceptualize movement patterns by segmenting them into meaningful parts. Route directions are composed using a plethora of cognitive spatial organization principles: changing levels of granularity, hierarchical organization, incorporation of cognitively and perceptually salient elements, and so forth. Identifying such information in text documents automatically is crucial for enabling machine-understanding of human spatial language. The benefits are: a) creating opportunities for large-scale studies of human linguistic behavior; b) extracting and georeferencing salient entities (landmarks) that are used by human route direction providers; c) developing methods to translate route directions to sketches and maps; and d) enabling queries on large corpora of crawled/analyzed movement data. In this paper, we introduce our approach and implementations that bring us closer to the goal of automatically processing linguistic route directions. We report on research directed at one part of the larger problem, that is, extracting the three most critical parts of route directions and movement patterns in general: origin, destination, and route parts. We use machine-learning based algorithms to extract these parts of routes, including, for example, destination names and types. We prove the effectiveness of our approach in several experiments using hand-tagged corpora.

  5. Chemical composition of lucerne leaf extract (EFL) and its applications as a phytobiotic in human nutrition.

    Science.gov (United States)

    Gaweł, Eliza

    2012-01-01

    Lucerne, a valuable plant grown mainly for animal feed, is rich in protein, minerals (Ca, Cu, Fe, Mg, Mn, P, Zn, Si), vitamins (A, B, C, D, E, K, U), phytochemical substances (carotene, chlorophyll, coumarins, isoflavones, alkaloids, saponins), contains secondary metabolites of plants (phytoestrogens: isoflavones and coumestrol), and antinutritional components (phytates, L-canavanine, saponins). It may be used as a dietary supplement in human nutrition. The proteins found in lucerne are comprised of numerous exogenous and semi-exogenous amino acids which are desirable for human body. Extract from the leaves of alfalfa (EFL - l'Extrait Foliaire de Luzerne) practically does not contain mycotoxins and pesticide pollutants. It is a completely natural product, safe even in a long-term supplementation. Extract from the leaves of alfalfa has a positive, multidirectional impact on the human body. It increases the level of estrogen, prevents atherosclerosis, helps blood circulation and strenghtens immunity, protects against the development of dangerous diseases of the digestive tract, combats anemia and many other health ailments. The results of preclinical studies indicate that alfalfa leaf extract enriched with vitamin C (EFL) can be a dietary supplement supporting the human body in fighting malnutrition, ischemic diseases, and various disorders of digestive tract. It also strengthens and enhances immunity.

  6. Chemical composition of lucerne leaf extract (EFL and its applications as a phytobiotic in human nutrition

    Directory of Open Access Journals (Sweden)

    Eliza Gaweł

    2012-09-01

    Full Text Available   Lucerne, a valuable plant grown mainly for animal feed, is rich in protein, minerals (Ca, Cu, Fe, Mg, Mn, P, Zn, Si, vitamins (A, B, C, D, E, K, U, phytochemical substances (carotene, chlorophyll, coumarins, isoflavones, alkaloids, saponins, contains secondary metabolites of plants (phytoestrogens: isoflavones and coumestrol, and antinutritional components (phytates, L-canavanine, saponins. It may be used as a dietary supplement in human nutrition. The proteins found in lucerne are comprised of numerous exogenous and semi-exogenous amino acids which are desirable for human body. Extract from the leaves of alfalfa (EFL – l’Extrait Foliaire de Luzerne practically does not contain mycotoxins and pesticide pollutants. It is a completely natural product, safe even in a long-term supplementation. Extract from the leaves of alfalfa has a positive, multidirectional impact on the human body. It increases the level of estrogen, prevents atherosclerosis, helps blood circulation and strenghtens immunity, protects against the development of dangerous diseases of the digestive tract, combats anemia and many other health ailments. The results of preclinical studies indicate that alfalfa leaf extract enriched with vitamin C (EFL can be a dietary supplement supporting the human body in fighting malnutrition, ischemic diseases, and various disorders of digestive tract. It also strengthens and enhances immunity.  

  7. 必兰浸润麻醉与利多卡因阻滞麻醉在下颌阻生齿微创拔牙术中的临床对照研究%A comparison of the clinical anesthetic efficacy of articaine infiltration and lidocaine blocking for microport extraction of impacted mandibular molar

    Institute of Scientific and Technical Information of China (English)

    黄元丁; 夏辉; 李晓东; 杨小竺; 裴仲秋; 夏熹

    2011-01-01

    Objective To compare the clinical efficacy of the infiltration anesthesia with primacaine and the nerve blocking anesthesia with lidocaine for microport extraction of impacted lower third molar. Methods 104 chosen patients had both sides of impacted lower third molars extracted in this study. Patients were given local anesthesia with either primacaine or lidocaine randomly at each side, and then underwent microport extraction. Clinical factors including effective proportion (EP), effecting time point (ETP), visual analogue scale of pain (VASp), alteration of systolic pressures (ASP) and analgesia duration (AD) were evaluated statistically by means of paired t-test. Results The EP of experimental group was higher than the control group (P=0.024). The ETP of soft tissue and alveoli-dental pulp was (1.04±0.21), (2.44±2.60)min in the experimental group, and much earlier than that of the control group(P=0.002,P=0.032). The VASp and ASP of experimental group were lower than the control group(P=0.041, P=0.018). AD was (103.6±35.5) min, and higher than the control group (P=0.04). Conclusion The infiltration anesthesia with primacaine has been proven to be a easier, reliable and quick-acting method. We suggest it an alternative method replacing the 2% lidocaine blocking during microport extraction of impacted lower third molar.%目的 比较必兰浸润麻醉与利多卡因阻滞麻醉法在下颌第三磨牙阻生齿微创拔牙术中的疗效.方法 选择双侧阻生齿拔除患者104名,在同一患者双侧下颌分别行必兰浸润麻醉或2%利多卡因阻滞麻醉,并行高速涡轮机微创拔牙,比较2组的麻醉显效率、麻醉显效时间、术中疼痛程度和术前后收缩压差及无痛持续时间,按配对样本t检验法行统计分析.结果 实验组麻醉显效率高于对照组(P=0.024).实验组软组织麻醉显效时间和牙槽突、牙髓麻醉显效时间分别为(1.04±0.21)、(2.44±2.60)min,较对照组均有降低(P=0.002,P=0

  8. Clinical Evaluation of the Extraction of Impacted Mandibular Third Molar by High-speed Turbine Assisted by Intraoral Camera System%口腔内镜辅助高速涡轮钻拔除阻生智齿的临床研究

    Institute of Scientific and Technical Information of China (English)

    黄田河; 刘继华

    2012-01-01

    Objective To observe the clinical curative effects of high - speed air turbine in extraction of impacted mandibular third molar assisted by intraoral camera system. Methods A total of 280 impacted mandibular third molars included in this study were randomly assigned to two groups:the experimental group and the control group,to which the high - speed turbine assisted by intraoral camera system method and high - speed turbine method were applied respectively. The average extraction time and postoperative complications such as swelling, pain, limition of mouth opening, dry socket ect, were compared between the two groups. Chi - square test and ( test were usedto statistical analysis. Results No sinigficant difference was noted in distribution mandibular third molar between two groups (P >0.05). The average extraction time was shorter, and the incidence of postoperative complications except dry socket was lower in experimental group than in control, and the difference was significant ( P <0. 05) . Conclusion The high-speed turbine assisted by intraoral camera system can sinigficantly shorten operative time and reduce postoperative complications during the extraction of wisdom teeth,which is worthy to be generalized in clinic.%目的 探讨口腔内镜辅助高速涡轮牙钻法拔除下颌阻生智齿的临床疗效.方法 随机选取280例需拔除下颌阻生智齿的患者,分为口腔内镜辅助高速涡轮钻(实验组,130例)和单纯高速涡轮牙钻法(对照组,150例).对比观察两组平均手术时间及术后肿胀、疼痛、张口受限及干槽症等并发症的发生情况.结果 实验组与对照组患者下颌阻生智齿分布比较,差异无统计学意义(P>0.05).实验组患者下颌阻生智齿平均拔除时间较对照组明显缩短,差异有统计学意义(P<0.05).实验组术后肿胀、疼痛及张口受限等并发症发生率明显低于对照组,差异有统计学意义(P<0.05).实验组与对照组干槽症发生

  9. A Comparison of the Clinical Efficacy of Extraction of Impacted Mandibular Third Molars Using High-speed ;Air-turbine Drill and the Traditional Dental Method%两种方式拔除下颌第三磨牙阻生的对比研究

    Institute of Scientific and Technical Information of China (English)

    钟守鹏

    2016-01-01

    Objective To evaluate the feasibility and safety of using high-speed air-turbine drill to extract medium and low impacted mandibular third molars. Methods 369 cases were randomly divided into control group and experiment group. Tra-ditional hammer chisel splitting method was used in the control group and high-speed air-turbine drill in the experiment group. Lim-ited mouth opening, postoperative pain and dry sockets were observed. Results The duration of tooth extraction in the experiment group is significantly shorter than that in the control group(P<0. 05). The pain score and limited mouth opening between the two groups demonstrated significant difference(P<0. 05). Also postoperative complications such as dry sockets is obviously lower in the experiment group(P<0. 05). Conclusion The high-speed air-turbine drill shows obvious advantages in the extraction of low and medium impacted mandibular