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Sample records for extract aloe arborescens

  1. Aloe arborescens aqueous gel extract alters the activities of key ...

    African Journals Online (AJOL)

    The present study investigated the antidiabetic activity and the possible mechanisms of action of aqueous extract of Aloe arborescens leaf gel (AALGEt) on normal and alloxan-induced diabetic rats. Diabetes was induced in 12 h fasted rats by intraperitoneal injection of 140 mg/kg body weight of alloxan. Blood glucose ...

  2. Final report on the safety assessment of AloeAndongensis Extract, Aloe Andongensis Leaf Juice,aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice,aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract.

    Science.gov (United States)

    2007-01-01

    Plant materials derived from the Aloe plant are used as cosmetic ingredients, including Aloe Andongensis Extract, Aloe Andongensis Leaf Juice, Aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice, Aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract. These ingredients function primarily as skin-conditioning agents and are included in cosmetics only at low concentrations. The Aloe leaf consists of the pericyclic cells, found just below the plant's skin, and the inner central area of the leaf, i.e., the gel, which is used for cosmetic products. The pericyclic cells produce a bitter, yellow latex containing a number of anthraquinones, phototoxic compounds that are also gastrointestinal irritants responsible for cathartic effects. The gel contains polysaccharides, which can be acetylated, partially acetylated, or not acetylated. An industry established limit for anthraquinones in aloe-derived material for nonmedicinal use is 50 ppm or lower. Aloe-derived ingredients are used in a wide variety of cosmetic product types at concentrations of raw material that are 0.1% or less, although can be as high as 20%. The concentration of Aloe in the raw material also may vary from 100% to a low of 0.0005%. Oral administration of various anthraquinone components results in a rise in their blood concentrations, wide systemic distribution, accumulation in the liver and kidneys, and excretion in urine and feces; polysaccharide components are distributed systemically and metabolized into smaller molecules. aloe-derived material has fungicidal, antimicrobial, and antiviral activities, and has been effective in wound healing and infection treatment in animals. Aloe barbadensis (also known as Aloe vera)-derived ingredients were not toxic

  3. A biochemical and cellular approach to explore the antiproliferative and prodifferentiative activity of Aloe arborescens leaf extract.

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    Di Luccia, Blanda; Manzo, Nicola; Vivo, Maria; Galano, Eugenio; Amoresano, Angela; Crescenzi, Elvira; Pollice, Alessandra; Tudisco, Raffaella; Infascelli, Federico; Calabrò, Viola

    2013-12-01

    Aloe arborescens Miller, belonging to the Aloe genus (Liliaceae family), is one of the main varieties of Aloe used worldwide. Although less characterized than the commonest Aloe vera, Aloe arborescens is known to be richer in beneficial phytotherapeutic, anticancer, and radio-protective properties. It is commonly used as a pharmaceutical ingredient for its effect in burn treatment and ability to increase skin wound healing properties. However, very few studies have addressed the biological effects of Aloe at molecular level. The aim of the research is to provide evidences for the antiproliferative properties of Aloe arborescens crude leaf extract using an integrated proteomic and cellular biological approach. We analysed the composition of an Aloe arborescens leaf extract by gas chromatography-mass spectrometry analysis. We found it rich in Aloe-emodin, a hydroxylanthraquinone with known antitumoral activity and in several compounds with anti-oxidant properties. Accordingly, we show that the Aloe extract has antiproliferative effects on several human transformed cell lines and exhibits prodifferentiative effects on both primary and immortalized human keratinocyte. Proteomic analysis of whole cell extracts revealed the presence of proteins with a strong antiproliferative and antimicrobial activity specifically induced in human keratinocytes by Aloe treatment supporting its application as a therapeutical agent. Copyright © 2013 John Wiley & Sons, Ltd.

  4. Phytochemical analysis and acaricidal activity of Aloe arborescens Mill. extracts against Rhipicephalus (Boophilus microplus

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    Aldair Calistro de Matos

    2017-10-01

    Full Text Available The indiscriminate use of chemical acaricides has allowed Rhipicephalus (Boophilus microplus to develop resistance to several active principles. However, botanical extracts have been tested as an alternative method to control those ticks. This experiment studied the chemical fingerprint and acaricidal effect of fresh and dry Aloe arborescens Mill. extracts on R. (B. microplus. The acaricidal activity of extracts was assessed using in vitro assays with engorged females, and phytochemical characterization was performed by infrared (IR spectroscopy and high-performance liquid chromatography (HPLC. The results showed that fresh and dry A. arborescens extracts prepared with the solvents pure ethanol, ethanol-dichloromethane binary mixture, and ethanol-dichloromethane-acetone ternary mixture, contained water-soluble tannins and had a strong effect on the reproductive parameters of R. (B. microplus demonstrated by a marked decreased in the number of eggs laid and in the larvae hatching rate (p < 0.05, p < 0.01, p < 0.001. In conclusion, A. arborescens Mill. has components with acaricidal activity against R. (B. microplus, and phytotherapy with extracts of this plant may be used as an alternative method of R. (B. microplus control.

  5. Antifungal activity evaluation of Aloe arborescens dry extract against trichosporon genus yeasts

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    João Ricardo Bueno de Morais Borba

    2014-10-01

    Full Text Available The objective of this study was to investigate the antifungal activity of Aloe arborescens dry extract against Trichosporon genus yeast species. Extraction was carried out by means of a longitudinal incision in fresh leaves, which were collected on a vat, and the total volume was frozen and subsequently lyophilized. Then, 40 mg of the dry extract was dissolved in DMSO by gentle inversion in order to obtain a solution whose concentration was 4000 µg mL-1. This solution became limpid and slightly yellowish because the pigment of the latex was attenuated. It was performed serial dilutions from 2,000 to 15.625 µg mL-1 with RPMI-1640 broth. There was already no pigment in the first dilution of 2000 μg mL-1. It was analyzed fifteen strains of Trichosporon spp., and Candida albicans ATCC 10231 was used as control strain. We carried out the reading of microplates in the ELISA reader device at a wavelength of 530 nm, after incubation for 24 and 48 hours, and it was determinated the Minimum Inhibitory Concentration (MIC. The MIC50 value obtained for all Trichosporon species and for C. albicans was 500 µg mL-1. As a result, we concluded that Aloe arborescens dry extract has antifungal activity against Trichosporon yeasts.

  6. Physicochemical and microbiological properties as well as stability of ointments containing aloe extract (Aloe arborescens Mill.) or aloe extract associated to neomycin sulphate.

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    Kodym, A; Bujak, T

    2002-12-01

    The aim of the study was to work out methods of quality assessment of ointments containing dry extract from fresh leaves of Aloe arborescens Mill. (Lilliaceae) and also of ointments containing both of dry extract and neomycin sulphate. The stability of the ointments, stored at 20 degrees C, was studied and the following criteria were considered: chromatographic analysis (TLC), pH of the ointments, the content of the substances in the dry extract converted to aloenin, the content of aloenin and aloin, anti-microbial activity of neomycin in the ointments, the size of the particles of the dry extract and of neomycin sulphate in the ointment suspension and the sterility of the ointments. After two years of storage at 20 degrees C, the ointments prepared with the anhydrous lipophilic base, did not change their physicochemical characteristics and neomycin in those ointments retained almost 100% of starting anti-microbial activity. Water or propylene glycol significantly decreased the stability of the biologically active substances of the dry extract in the ointments. Besides, in the ointments containing the dry extract and neomycin sulphate, the presence of water or propylene glycol induced degradation of the biologically active substances of the dry extract and a decrease in the anti-microbial activity of neomycin in the ointments. Considering the physicochemical and microbiological stability, the most advisable base for the ointments with aloe and neomycin sulphate was composed of white vaseline, liquid paraffin, solid paraffin, cholesterol.

  7. The content of secondary phenol metabolites in pruned leaves of Aloe arborescens, a comparison between two methods: leaf exudates and leaf water extract.

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    Gutterman, Yitzchak; Chauser-Volfson, Elena

    2008-10-01

    Aloe arborescens plants, originating from the deserts of South Africa, are grown in the Introduction Garden at Sede Boker in the Negev Desert of Israel. In previous studies, we developed agro-technical methods to raise the content of secondary phenol metabolites (SPhMs) in the Aloe leaves. Plants that are subjected to repeated leaf pruning respond by increasing the content of their SPhMs. The SPhMs found in Aloe arborescens include barbaloin, aloenin and derivatives of aloeresin. Such compounds are used for many purposes, including human skin protection from sun and fire burns and high radiation, as products of the pharmaceutics and cosmetics industries, and as food supplements for treating stomach ulcers and diabetes. In the current study, the SPhMs were separated from pruned leaves of the same A. arborescens plants at the same time by two methods: (1) exudation by squeezing the tissues of the leaves, (2) immersion of the leaves' pruned cut bottom in water and collection of the extract. The exudates and extract were frozen, freeze-dried to a powder and the SPhMs were then separated by chromatography. The yield of powder from water extraction from pruned leaves was much lower than the yield from the exudates. However, higher percentages of the powder from the water extraction contained SPhMs (between 80 and 92.7%). The content of powder in leaf exudates from pruned leaves was much higher because the SPhMs were squeezed out from the cells and tissues. However, the percentages of SPhMs in this powder were much lower (between 39 and 62%).

  8. Antiviral activity of an aqueous extract derived from Aloe arborescens Mill. against a broad panel of viruses causing infections of the upper respiratory tract.

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    Glatthaar-Saalmüller, B; Fal, A M; Schönknecht, K; Conrad, F; Sievers, H; Saalmüller, A

    2015-09-15

    A number of antiviral therapies have evolved that may be effectively administered to treat respiratory viral diseases. But these therapies are very often of limited efficacy or have severe side effects. Therefore there is great interest in developing new efficacious and safe antiviral compounds e.g. based on the identification of compounds of herbal origin. Since an aqueous extract of Aloe arborescens Mill. shows antiviral activity against viruses causing infections of the upper respiratory tract in vitro we hypothesised that a product containing it such as Biaron C(®) could have an antiviral activity too. Antiviral activity of Bioaron C(®), an herbal medicinal product consisting of an aqueous extract of Aloe arborescens Mill., Vitamin C, and Aronia melanocarpa Elliot. succus, added as an excipient, was tested in vitro against a broad panel of viruses involved in upper respiratory tract infections. These studies included human adenovirus and several RNA viruses and were performed either with plaque reduction assays or with tests for the detection of a virus-caused cytopathic effect. Our studies demonstrated an impressive activity of Bioaron C(®) against members of the orthomyxoviridae - influenza A and influenza B viruses. Replication of both analysed influenza A virus strains - H1N1 and H3N2 - as well as replication of two analysed influenza B viruses - strains Yamagatal and Beiying - was significantly reduced after addition of Bioaron C(®) to the infected cell cultures. In contrast antiviral activity of Bioaron C(®) against other RNA viruses showed a heterogeneous pattern. Bioaron C(®) inhibited the replication of human rhinovirus and coxsackievirus, both viruses belonging to the family of picornaviridae and both representing non-enveloped RNA viruses. In vitro infections with respiratory syncytial virus and parainfluenza virus, both belonging to the paramyxoviridae, were only poorly blocked by the test substance. No antiviral activity of Bioaron C(®) was

  9. Preliminary evaluation: the effects of Aloe ferox Miller and Aloe arborescens Miller on wound healing.

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    Jia, Yimei; Zhao, Guodong; Jia, Jicheng

    2008-11-20

    Genus Aloe has been traditionally utilized for medicinal purpose for decades. Compared with Aloe vera gel, the qualitative assessment for the therapeutic effects of the other two Aloe species, Aloe ferox Miller and Aloe arborescens Miller, for their topical wound healing was less addressed. Therefore, the aim of present study is to provide the positive evidence for Aloe ferox Miller and Aloe arborescens Miller supporting their therapeutic properties for topical treatment of skin wounds. Two types of the whole-leaf juice prepared from either Aloe ferox Miller or Aloe arborescens Miller were used in this study. Incision wound healing was investigated using both the rat and rabbit model. The wound closure rate with and without the topical administration of the whole-leaf juice were monitored. The changes in wound characteristics were traced and wound severity was scored on different days. The anti-microorganism actions of each whole-leaf juice preparation were evaluated by measuring their inhibition growth effects on four bacterial strains and three fungal spores. The toxic influence owing to topical application of Aloe whole-leaf juice on intact and damaged skin was also assessed. Our results indicated that the two types of whole-leaf juice preparations exhibit the therapeutic properties, including facilitation of the healing process, selective inhibition of the microbial growth and zero side-effect on the skin, during the observation period. It is concluded that both of Aloe whole-leaf juice preparations have the positive potential for skin medicinal application.

  10. Chromatographic separation and detection methods of Aloe arborescens Miller constituents: A systematic review.

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    Nazeam, Jilan A; Gad, Haidy A; El-Hefnawy, Hala M; Singab, Abdel-Naser B

    2017-07-15

    Aloe arborescens Miller (Family Asphodelaceae) is a member of genus Aloe, which is used in traditional medicine to cure various diseases. The extracts of the plant have been reported to possess anticancer, immunomodulator, antidiabetic, anti-inflammatory and antioxidant activities. The phytochemical investigations have revealed diverse chemical constituents, including phenolics [anthraquinones, anthrones, pyrones, chromones and coumarins], polysaccharides [arborans [(1-4) linked glucomannans, polysaccharide (A, B and C): (A: a linear (1-6)-O-α-glucan, B: a branching (1-2)-O-l-arabinose with (1-2)-O-d-galactose linkages and C: (1-4)-O-β-mannan with 18% acetyl group)

  11. Neuroprotective potential of Aloe arborescens against copper induced neurobehavioral features of Parkinson's disease in rat.

    Science.gov (United States)

    Abbaoui, Abdellatif; Hiba, Omar El; Gamrani, Halima

    2017-06-01

    Copper (Cu) is an important trace element for the organism survival, which ensures the normal functioning of different biosystems. However, excessive levels of this heavy metal are responsible for profound physiological alterations including the central nervous system. Numerous findings sustain the involvement of heavy metals, as an environmental risk factor such as copper (Cu), in the neuropathology of Parkinson's disease (PD) which is a chronic neurodegenerative disorder that principally affects the motor system. The classic and evident symptoms of PD namely rigidity, tardiness of movement, and difficulty with walking, result from progressive dopaminergic neurons death within substantia nigra. Whereas, few pharmacological trials have shown a beneficial role against Cu neurotoxicity, Aloe arborescens is one of the powerful medicinal plants with an array of therapeutic effects. Thus, we aimed through the present study, to evaluate the impact of acute Cu intoxication (10μg/g B.W. i.p) for 3days on the dopaminergic system and locomotor performance, together with the possible restorative effect of oral administration of aqueous extract of Aloe arborescens gel (AEAAG) (200mg/kg B.W.). By means of immunohistochemistry, we noted, in the Cu intoxicated rats, a significant loss of TH (tyrosine hydroxylase) expression within substantia nigra compacta (SNc), ventral tegmental area (VTA) and the subsequent striatal outputs, those alterations were correlated to behavioral abnormalities such as a severe drop of locomotor performance. While AEAAG administration to Cu intoxicated rats showed a noticeable beneficial effect; this potential was featured by a complete recovery of the TH expression and locomotor behavior deficiencies in the intoxicated rats. The present investigation have brought, on the one hand, an experimental evidence of an altered dopaminergic innervations following Cu intoxication and on the other hand, a new pharmacological property of Aloe arborescens that

  12. Aloe arborescens Polysaccharides: In Vitro Immunomodulation and Potential Cytotoxic Activity.

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    Nazeam, Jilan A; Gad, Haidy A; Esmat, Ahmed; El-Hefnawy, Hala M; Singab, Abdel-Naser B

    2017-05-01

    Different polysaccharides were isolated from the leaves of Aloe arborescens using the gradient power of hydrogen followed by antitumor and immunomodulatory assay. The total polysaccharide content of different fractions, water-soluble polysaccharide (WAP), acid-soluble polysaccharide (ACP), and alkaline-soluble polysaccharide (ALP), was estimated using a phenol-sulfuric acid spectrophotometric method. WAP possessed a higher content of mannose and glucose than either ACP or ALP. In vitro antitumor activity was investigated in three different cancer cell lines, and in vitro immunomodulatory potential was assessed through phagocytosis and lymphocyte transformation assay. The results showed that WAP and ALP exhibited the most significant cytotoxicity against HepG2 human liver cancer cells, with IC 50 values of 26.14 and 21.46 μg/mL, respectively. In contrast, ALP was able to enhance lymphocyte transformation, whereas WAP had the most potent phagocytic activity. Molecular weight, total sugar and uronic acid content, Fourier transform-infrared analysis, and linkage type of bioactive polysaccharides were investigated. These findings revealed that the potential antitumor activity of the natural agents WAP and ALP was through an immunomodulation mechanism, which verifies the use of the plant as adjuvant supplement for cancer patients suffering immunosuppression during chemotherapy.

  13. Biologically active constituents of leaves and roots of Aloe arborescens var. natalensis.

    Science.gov (United States)

    Hirata, T; Suga, T

    1977-01-01

    Several biologically active substances, such as aloenin (1), magnesium lactate, aloe-emodin (4), barbaloin (5), and succinic acid, were found to be contained in the leaf juice of Aloe arborescens Mill. var. natalensis Berger, which has widely been used in domestic medicines. Aloenin (1) and magnesium lactate were elucidated to exhibit an inhibitory action on the gastric juice secretion of rats. Various constituents other than the above bioactive substances were found in the leaves and the roots of the plant.

  14. TECHNOLOGICAL ASPECTS CONCERNING PRODUCTION ON ASEXUAL TRACK OF ORNAMENTAL CULTURE OF ALOE ARBORESCENS

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    Mădălina Doltu

    2012-12-01

    Full Text Available Research conducted in culture of Aloe arborescens Mill., in conditions of protected area (greenhouse, had to watch some of technological aspects to obtain some in pot ornamentals plants, competitive plants for european market. Experience has included plants of Aloe arborescens Mill., aged 1-5 years, obtained by vegetative multiplying, asexual. Production technology was applied to aloe plants (multiplying, transplanting, maintenance, taking into account the ecological requirements of specie and possibilities of using automated artificial factors influencing microclimate (shading, ventilation, cooling of production space. Researches has established technological aspects concerning production of plants, to satisfy requirements of specie, for obtained some specimens by superior decorative quality. The results of experienced technology indicate quality of morphological characters, growth increases important of decorative elements (height, leaves, shoots, appearance of flowering at plants aged two years. Culture of Aloe arborescens Mill. in pot, can be practiced all year, just in conditions of protected areas. This culture, realized in the purpose for obtain decorative plants, is a valuable activity of horticulture.

  15. Inhibition of azoxymethane-induced DNA adduct formation by Aloe arborescens var. natalensis.

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    Shimpo, Kan; Chihara, Takeshi; Beppu, Hidehiko; Ida, Chikako; Kaneko, Takaaki; Hoshino, Motoyuki; Kuzuya, Hiroshi

    2003-01-01

    To clarify the possible mechanisms of inhibition of azoxymethane (AOM)-induced aberrant crypt foci (ACF) in the rat colorectum by freeze-dried whole leaves of Aloe arborescens var. natalensis (Kidachi aloe) (hereinafter referred to as ALOE) and commercial crude aloin (Sigma A-0451; from Curacao aloe) (hereinafter ALOIN), we studied the effects of ALOE and ALOIN on the formation of AOM-induced DNA adducts (O6-methylguanine; O6-MeG) in rats. Male F344 rats (4 weeks old) were fed a basal diet, or experimental diets containing 5%ALOE or 0.25%ALOIN for 5 weeks. All rats were injected s.c. twice with 15 mg/kg AOM, once at the end of week 1, and once at the end of week 2. The animals were sacrificed 6 hours after the second injection to analyze DNA adducts (O6-MeG) in the colorectum. Dietary administration of ALOE significantly inhibited the O6-MeG levels (50% reduction) compared with controls, whereas the O6-MeG levels in the ALOIN-fed rats showed a tendency to decrease (by 30%), although not significantly. In this study, we also measured the enzyme activity and mRNA level of cytochrome (CYP) 2E1, known to be responsible for the activation of AOM, in rat liver. ALOE-fed rats showed significantly reduced CYP2E1 enzymatic activity (27% reduction) compared with controls. On the other hand, the activity in ALOIN-fed rats tended to decrease by 11%, although not significantly. The CYP2E1 mRNA levels in ALOE- and ALOIN-fed rats were slightly reduced (9.7% and 5.2%, respectively). These results may explain, at least in part, the previously observed inhibitory effects of ALOE and ALOIN, especially ALOE on AOM-induced ACF formation in the rat colorectum.

  16. Caracterización morfoanatómica comparativa entre Aloe vera (L. Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae

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    Stella Maris Carpano

    Full Text Available Aloe vera (L. Burm. F. (= Aloe barbadensis Miller conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies do mesmo gênero. Isto é o que ocorre com três espécies de Aloe spp., que se cultivam ou crescem acidentalmente na Argentina: A. arborescens Mill., A. saponaria Haw. e A. ciliaris Haw. (Aloaceae. Estabeleceram-se os caracteres morfoanatômicos de diagnóstico por microscopia óptica e microscopia eletrônica de varredura (apresentam-se desenhos e fotomicrografias dos mesmos. Obtiveram-se valores numéricos dos elementos histológicos de diagnóstico: magnitudes lineares e proporcionais. Realizaram-se reações histoquímicas de identificação dos princípios ativos para sua localização "in situ".

  17. A Systemic Review on Aloe arborescens Pharmacological Profile: Biological Activities and Pilot Clinical Trials.

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    Singab, Abdel-Naser B; El-Hefnawy, Hala M; Esmat, Ahmed; Gad, Haidy A; Nazeam, Jilan A

    2015-12-01

    Since ancient times, plants and herbal preparations have been used as medicine. Research carried out in the last few decades has verified several such claims. Aloe arborescens Miller, belonging to the Aloe genus (Family Asphodelaceae), is one of the main varieties of Aloe used worldwide. The popularity of the plant in traditional medicine for several ailments (antitumor, immunomodulatory, antiinflammatory, antiulcer, antimicrobial and antifungal activity) focused the investigator's interest on this plant. Most importantly, the reported studies have shown the plant effectiveness on various cancer types such as liver, colon, duodenal, skin, pancreatic, intestinal, lung and kidney types. These multiple biological actions make Aloe an important resource for developing new natural therapies. However, the biological activities of isolated compounds such as glycoprotein, polysaccharides, enzyme and phenolics were insufficient. Considering all these, this contribution provides a systematic review outlining the evidence on the biological efficacy of the plant including the pharmacology and the related mechanisms of action, with specific attention to the various safety precautions, and preclinical and clinical studies, indicating the future research prospects of this plant. Copyright © 2015 John Wiley & Sons, Ltd.

  18. Radical-scavenging effects of Aloe arborescens Miller on prevention of pancreatic islet B-cell destruction in rats.

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    Beppu, Hidehiko; Koike, Takaaki; Shimpo, Kan; Chihara, Takeshi; Hoshino, Motoyuki; Ida, Chikako; Kuzuya, Hiroshi

    2003-11-01

    We evaluated the possible scavenging effects of Aloe arborescens Miller var. natalensis Berger (Kidachi aloe in Japanese) on free radicals generated by streptozotocin (Sz) or alloxan (Ax). The components of Kidachi aloe were added to a reaction system in which .OH radicals derived from Sz or Ax as pancreatic islet B-cell toxins and hypoxanthine-xanthine oxidase (HX-XO)-derived O(2) radicals destroy isolated islet B-cells, and we observed its preventive effects. The Kidachi aloe components inhibited the destruction of rat pancreatic islet B-cells by Sz, Ax or HX-XO. These components were prepared in the form of a freeze-dried powder of the boiled leaf skin of Kidachi aloe, and measurement of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity showed higher radical-scavenging activity in this boiled leaf skin powder than the non-boiled leaf skin powder.Furthermore, HPLC chromatograms of the "Boiled leaf skin powder" were similar to those of commercially available aloin (barbaloin content: approximately 20%). Therefore, the main component may be a phenol compound. In addition, the phenolic fraction of the Boiled leaf skin contained large amounts of 2'-O-p-coumaroylaloesin and 2'-O-feruloylaloesin, which have higher DPPH radical-scavenging activity than barbaloin. These results suggest that the action mechanism of Kidachi aloe Boiled leaf skin components, which prevent destruction of the pancreatic islets by specific pancreatic islet toxins such as Sz, Ax, and HX-XO, involves inhibition of free radical-scavenging effects, and may be associated with a thermostable low molecular component. The co-existence of Kidachi aloe-derived 2'-O-p-coumaroylaloesin, 2'-O-feruloylaloesin, and aloin may result in the potentiation of radical-scavenging activity.

  19. [Radio protective drug production from fresh leaves of Aloe arborescens Mill].

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    Bakuridze, A Dzh; Nikolaev, S M; Berashvili, D T; Bakuridze, K A; Tsomaia, I V

    2009-06-01

    Nowadays, phytogenous drugs are wildly used as radio protective substances. The aim of the research was to study radio protective characteristics of aloe juice fraction and to develop new technology for radio protective drug production. Technological scheme for getting the drug in two stages. The first stage - extraction of juice from fresh leaves; the second stage - extracting bagasse have been developed and optimal environment for bagasse extraction are defined: Infusion of bagasse with 96 % ethyl spirit (1:1) during 30 minutes, continuation of extracting with water on correlation to raw materials 10:1 at temperature of 70 degrees C during 30 minutes. For the basis of the first series of balanced loading there are taken the optimal parameters of extracting process, on the basis of which in its turn was developed technological scheme of getting dry extract of aloe. Dry extract is a fine-dispersed reddish-yellow (brownish-yellow) powder, which can be easily dissolved in warm (40-60 degrees C) water. Pharmacological researches were conducted in the Institute of General and Experimental Biology, Siberian Branch, Russian. Academy of Sciences. The remarkable radio protective effect of the drug was revealed.

  20. Ultraviolet light absorption of an ophthalmic formulation with Aloe extracts.

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    Ji, Yimei; Jia, Jicheng

    2009-09-01

    Aloin and polysaccharide present in extracts of Aloe arborescens Miller were formulated into a binary solution to protect eyes from bacterial infection and ultraviolet radiation (UVR). The UVR absorption spectrum was recorded from 190 to 440 nm using a UV spectrometer. The physical properties of the product were examined in terms of its appearance, odor, pH, viscosity, density, refractive index, and stability. The binary solution exhibited three absorption peaks in the UVA, B and C regions, respectively. Such UV absorption capability was attributed to the phenolic chromophores pertaining to aloin. The present study suggested that the formulated binary solution has potential application as an UV absorption agent with built-in antimicrobial activity.

  1. Artemisia arborescens "Powis Castle" extracts and α-thujone prevent fruit infestation by codling moth neonates.

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    Creed, Cory; Mollhagen, Ariel; Mollhagen, Noelle; Pszczolkowski, Maciej A

    2015-01-01

    The codling moth, Cydia pomonella L. (Tortricidae), is a major cosmopolitan pest of the apple. The potential of plant-derived semiochemicals for codling moth control is poorly studied. To evaluate the potential of crude extracts of five plants from the Asteraceae family: Artemisia absinthium L., Artemisia arborescens L. "Powis Castle", Artemisia annua L., and Artemisia ludoviciana Nutt. to prevent apple infestation by C. pomonella larvae and to identify the deterrent(s) in these plants. Artemisia dried leaves were extracted in v/v mixture of 80% ethanol, 10% isopropanol, and 10% of methanol, and the extracts were analyzed using high-performance thin layer chromatography. Preference of fruit treated with test solutions (Artemisia extracts or α-thujone) versus fruit treated with solvent was studied using choice assays. α-Thujone was detected in A. arborescens extract at a concentration of 77.4 ± 2.4 mg/g of dry tissue, localized between Rf 0.75 and 0.79 and was absent from crude extracts of remaining Artemisia species. Material from each extract in the zone between Rf 0.75 and 0.79 was removed from chromatographic plates and tested for feeding deterrence. Only the material from A. arborescens showed feeding deterrent properties. Minimum concentrations that prevented fruit infestation were 10 mg/ml for α-thujone and 1 mg/ml for A. arborescens crude extract. Artemisia arborescens contains chemicals that prevent apple infestation by codling moth neonates. Thujone is one of these chemicals, but it is not the only constituent of A. arborescens crude extract that prevents fruit infestation by codling moth neonates.

  2. Antidiabetic effects of dietary administration of Aloe arborescens Miller components on multiple low-dose streptozotocin-induced diabetes in mice: investigation on hypoglycemic action and systemic absorption dynamics of aloe components.

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    Beppu, Hidehiko; Shimpo, Kan; Chihara, Takeshi; Kaneko, Takaaki; Tamai, Ikuko; Yamaji, Sachiyo; Ozaki, Sayaka; Kuzuya, Hiroshi; Sonoda, Shigeru

    2006-02-20

    We carried out three experimental trials to determine antidiabetic effects of Aloe arborescens Miller components. Firstly, ICR mice which received frequent injections of streptozotocin (Sz) in small doses (low-dose Sz-induced diabetes mice) were fed ad libitum with basal diets supplemented with components of Aloe arborescens Miller var. natalensis Berger (Kidachi aloe) and Aloe vera Linne from 31 days before to 73 days after the Sz injections. Variation in blood glucose levels, incidence rates of insulitis and blood insulin levels were examined during the trial. As a result, groups receiving diets supplemented at the rate of 2% with whole leaf of Kidachi aloe and 10 KDa fraction powder (a fraction with less than 10 KDa molecular weight derived from Kidachi aloe leaf skin juice by ultra filtration) significantly suppressed the elevation of blood sugar as compared to a control group receiving basal diet. In contrast, there was no significant effect with Aloe vera leaf pulp powder. Insulitis emerged at the rate of 87% in the basal diet group. On the contrary, the whole aloe leaf and 10 KDa fraction groups significantly decreased the incidence of insulitis and incidence rates of whole aloe leaf and 10 KDa fraction powder were 51 and 38%, respectively. While insulin levels in the basal diet group averaged at 0.05 ng, more than four times the insulin level was observed in the 10 KDa group relative to the basal diet group. Secondary, the inhibitory effects of test materials on intestinal glucose absorption were observed using the jejunum of rats. A strong inhibitory action on intestinal glucose absorption was observed in the 10 KDa fraction powder group. Thirdly, phenol compounds derived from aloe in the blood serum and organs were quantitatively measured by a HPLC following forced administration of aloe components to rats to determine absorption kinetics of aloe components inside the body. The primary component of aloe phenol compounds is the same component of the 10 KDa

  3. Aloe arborescens aqueous gel extract alters the activities of key ...

    African Journals Online (AJOL)

    Mogale

    2011-05-16

    May 16, 2011 ... be attributed to its effects on plasma insulin levels. Hepatic glycogenolysis and gluconeogenesis are major causes of fasting hyperglycemia seen in both type 1 and type 2 diabetes mellitus (Smith et al., 2005). Inhibition of enzymes involved in gluconeogenesis and/or glycol- genolysis therefore constitutes ...

  4. Análise sazonal do potencial antimicrobiano e teores de flavonoides e quinonas de extratos foliares de Aloe arborescens Mill., Xanthorrhoeaceae

    OpenAIRE

    Fernando Leite Cardoso; Cynthia Murakami; Marco Aurélio Sivero Mayworm; Lucas Miranda Marques

    2010-01-01

    Este trabalho teve como objetivos avaliar o potencial antimicrobiano e os teores de flavonoides e quinonas de extratos foliares de Aloe arborescens Mill., Xanthorrhoeaceae, produzidos em diferentes épocas do ano. Extratos etanólicos e clorofórmicos foram preparados a partir de folhas, os bioensaios de atividade antimicrobiana foram desenvolvidos pelo método de macrodiluição em caldo, e dosagens de flavonoides e quinonas foram realizadas nos extratos. Todos os extratos apresentaram ação inibit...

  5. Análise sazonal do potencial antimicrobiano e teores de flavonoides e quinonas de extratos foliares de Aloe arborescens Mill., Xanthorrhoeaceae

    OpenAIRE

    Cardoso, Fernando Leite; Murakami, Cynthia; Mayworm, Marco Aurélio Sivero; Marques, Lucas Miranda

    2010-01-01

    P. 35-40,Jan./Mar. Este trabalho teve como objetivos avaliar o potencial antimicrobiano e os teores de flavonoides e quinonas de extratos foliares de Aloe arborescens Mill., Xanthorrhoeaceae, produzidos em diferentes épocas do ano. Extratos etanólicos e clorofórmicos foram preparados a partir de folhas, os bioensaios de atividade antimicrobiana foram desenvolvidos pelo método de macrodiluição em caldo, e dosagens de flavonoides e quinonas foram realizadas nos extratos. Todos os extratos ap...

  6. Aloe plant extracts as alternative larvicides for mosquito control ...

    African Journals Online (AJOL)

    The larvicidal activity of extracts from Aloe turkanensis, Aloe ngongensis and Aloe fibrosa against the common malaria vector, Anopheles gambie, was determined. Ground Aloe leaves from the three plants were sequentially extracted with hexane, ethyl acetate, chloroform, acetone and methanol. Only the ethyl acetate ...

  7. Aloe plant extracts as alternative larvicides for mosquito control

    African Journals Online (AJOL)

    SERVER

    2008-04-03

    Apr 3, 2008 ... Key words: Aloe, anopheles gambie, larvicidal activity. INTRODUCTION. Extracts from plants in the genus Aloe (Aloeaceae) have been widely used by pharmaceutical and cosmetic industries. Aloe species have long been known as medicinal plants (Cheney, 1970) and Aloe vera species is most widely ...

  8. Chiral separation of hesperidin and naringin and its analysis in a butanol extract of Launeae arborescens.

    Science.gov (United States)

    Belboukhari, Nasser; Cheriti, Abdelkrim; Roussel, Christian; Vanthuyne, Nicolas

    2010-04-01

    Two flavanone glycosides were isolated from the aerial part of Launeae arborescens (Asteraceae), which were identified as hesperidin and naringin. They are the most abundant flavonoids in the edible parts of many species of citrus fruits. In this study, we were interested in the chiral separation and determination of the diastereomerisation barriers of hesperidin and naringin by HPLC methods. The chiral separation HPLC screening of diastereomers of hesperidin and naringin by HPLC methods was accomplished in the normal-phase mode using 11 chiral stationary phases and various n-hexane/alcohol mobile phases. The rate constants and activation energy of diastereomerisation (DeltaG#) of flavanones, naringin and hesperidin were determined, respectively, on Chiralpak IC and Chiralpak IA. The analysis of flavanones isolated in butanol extracts of Launeae arborescens were confirmed by HPLC on Chiralpak IC.

  9. Nephroprotective and antioxidant properties of Artemisia arborescens hydroalcoholic extract against oestroprogestative-induced kidney damages in rats.

    Science.gov (United States)

    Dhibi, Sabah; Bouzenna, Hafsia; Samout, Noura; Tlili, Zied; Elfeki, Abdelfettah; Hfaiedh, Najla

    2016-08-01

    Currently, medicinal plants are found to have biological and pharmacological activities and are used in various domains. This study, carried out on Wistar rats, evaluates the beneficial effects of Artemisia arborscens extract on oestroprogestative-induced damages in kidney. Thirty-six 3-month-old Wistar rats were divided into 4 batches of nine each: a control group, a group of rats receiving oestroprogestative treatment, a group undergoing oestroprogestative treatment after receiving Artemisia arborescens extract in drinking water, and a group that received only Artemisia arborescens. Artemisia arborescens extract was found to optimize many parameters which were shifted to pathological values as a consequence of oestroprogestative toxicity: plasma creatinine and urea levels were decreased, uric acid and proteins were restored to normal values. The alteration of renal architecture was also suppressed. In addition, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities that had been reduced in kidney of the treated group were restored by Aretmisia arborscens-based treatments and, therefore, the lipid peroxidation level was reduced in the renal tissue compared to the control group. The obtained results confirmed that the Artemisia-based treatment allowed efficient protection against oestroprogestative-induced nephrotoxicity by restoring the activities of kidney. The protective effect of Artemisia arborescens was mainly attributed to antioxidant properties as well as the presence of phenolic acids and flavonoids detected by means of HPLC. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  10. Análise sazonal do potencial antimicrobiano e teores de flavonoides e quinonas de extratos foliares de Aloe arborescens Mill., Xanthorrhoeaceae

    Directory of Open Access Journals (Sweden)

    Fernando Leite Cardoso

    Full Text Available Este trabalho teve como objetivos avaliar o potencial antimicrobiano e os teores de flavonoides e quinonas de extratos foliares de Aloe arborescens Mill., Xanthorrhoeaceae, produzidos em diferentes épocas do ano. Extratos etanólicos e clorofórmicos foram preparados a partir de folhas, os bioensaios de atividade antimicrobiana foram desenvolvidos pelo método de macrodiluição em caldo, e dosagens de flavonoides e quinonas foram realizadas nos extratos. Todos os extratos apresentaram ação inibitória sobre os microrganismos testados. O extrato clorofórmico de inverno apresentou a menor CIM (128 µg/mL sobre B. subtilis. Os extratos clorofórmicos de inverno, primavera e verão apresentaram maior atividade antimicrobiana em relação ao extrato clorofórmico de outono. O extrato etanólico de inverno apresentou a menor CIM (256 µg/mL e a menor CMM (512 µg/mL sobre K. pneumoniae. Os extratos etanólicos de verão e outono mostraram baixa atividade antimicrobiana. Os teores de quinonas das folhas foram maiores nos períodos mais quentes de coleta (verão e outono, enquanto os teores de flavonoides foram semelhantes nos quatro períodos de coleta.

  11. Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

    Science.gov (United States)

    Mi Lee, Eun; Bai, Hyoung-Woo; Sik Lee, Seung; Hyun Hong, Sung; Cho, Jae-Young; Yeoup Chung, Byung

    2012-08-01

    Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  12. Caracterización morfoanatómica comparativa entre Aloe vera (L.) Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae) : Comparative morphoanatomical characterization between Aloe vera (L.) Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. and Aloe ciliaris Haw. (Aloaceae)

    OpenAIRE

    Stella Maris Carpano; María Teresa Castro; Etile Dolores Spegazzini

    2009-01-01

    Aloe vera (L.) Burm. F. (= Aloe barbadensis Miller) conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies d...

  13. Prevention of the onset of hyperglycaemia by extracts of Aloe ...

    African Journals Online (AJOL)

    GREGO

    Short Communication. Prevention of the onset of hyperglycaemia by extracts ... Key words: Aloe barbadensis, alloxan, fasting plasma glucose, hyperglycaemia. INTRODUCTION. Aloe barbadensis has been ... between means was determined using the student's t-test and. P<0.05 was considered to represent significant ...

  14. In vivo diabetic wound healing effect and HPLC–DAD–ESI–MS/MS profiling of the methanol extracts of eight Aloe species

    Directory of Open Access Journals (Sweden)

    Abeer M. El Sayed

    Full Text Available Abstract Genus Aloe, Xanthorrhoeaceae, is well distributed all over Egypt, and many species have been used as medicinal plants; mainly reported to prevent cardiovascular diseases, cancer and diabetes. This study attempts to analyze the secondary metabolites in the methanol extract of the leaves of eight Aloe species; A. vera (L. Burm. f., A. arborescens Mill., A. eru A. Berger, A. grandidentata Salm-Dyck, A. perfoliata L., A. brevifolia Mill., A. saponaria Haw. and A. ferox Mill. growing in Egypt. For this aim HPLC–DAD–MS/MS in negative ion mode was used. Although belonging to the same genus, the composition of each species presented different particularities. Seventy one compounds were identified in the investigated Aloe species, of which cis-p-coumaric acid derivaties, 3,4-O-(E caffeoylferuloylquinic acid and caffeoyl quinic acid hexoside were the most common phenolic acids identified. Aloeresin E and isoaloeresin D, 2'-O-feruloylaloesin were the common anthraquinones identified. Lucenin II, vicenin II, and orientin were the common identified flavonoids in the investigated Aloe species. 6'-Malonylnataloin, aloe-emodin-8-O-glucoside, flavone-6,8-di-C-glucosides could be considered as chemotaxonomic markers for the investigated Aloe species. The eight Aloe species had significant anti-inflammatory activity, in addition to the significant acceleration of diabetic wound healing in rats following topical application of the methanol extracts of their leaves. This is the first simultaneous characterization and qualitative determination of multiple phenolic compounds in Aloe species from locally grown cultivars in Egypt using HPLC–DAD–MS/MS, which can be applied to standardize the quality of different Aloe species and the future design of nutraceuticals and cosmetic preparations.

  15. Aloe vera extract activity on human corneal cells.

    Science.gov (United States)

    Woźniak, Anna; Paduch, Roman

    2012-02-01

    Ocular diseases are currently an important problem in modern societies. Patients suffer from various ophthalmologic ailments namely, conjunctivitis, dry eye, dacryocystitis or degenerative diseases. Therefore, there is a need to introduce new treatment methods, including medicinal plants usage. Aloe vera [Aloe barbadensis Miller (Liliaceae)] possesses wound-healing properties and shows immunomodulatory, anti-inflammatory or antioxidant activities. NR uptake, MTT, DPPH• reduction, Griess reaction, ELISA and rhodamine-phalloidin staining were used to test toxicity, antiproliferative activity, reactive oxygen species (ROS) reduction, nitric oxide (NO) and cytokine level, and distribution of F-actin in cells, respectively. The present study analyzes the effect of Aloe vera extracts obtained with different solvents on in vitro culture of human 10.014 pRSV-T corneal cells. We found no toxicity of ethanol, ethyl acetate and heptane extracts of Aloe vera on human corneal cells. No ROS reducing activity by heptane extract and trace action by ethanol (only at high concentration 125 µg/ml) extract of Aloe vera was observed. Only ethyl acetate extract expressed distinct free radical scavenging effect. Plant extracts decreased NO production by human corneal cells as compared to untreated controls. The cytokine (IL-1β, IL-6, TNF-α and IL-10) production decreased after the addition of Aloe vera extracts to the culture media. Aloe vera contains multiple pharmacologically active substances which are capable of modulating cellular phenotypes and functions. Aloe vera ethanol and ethyl acetate extracts may be used in eye drops to treat inflammations and other ailments of external parts of the eye such as the cornea.

  16. [Effects of aloe extracts, aloctin A, on gastric secretion and on experimental gastric lesions in rats].

    Science.gov (United States)

    Saito, H; Imanishi, K; Okabe, S

    1989-05-01

    Effect of aloctin A, glycoprotein isolated from leaves of Aloe arborescens MILL, on gastric secretion and on acute gastric lesions in rats were examined. Aloctin A given intravenously dose-dependently inhibited the volume of gastric juice, acid and pepsin output in pylorus-ligated rats. Aloctin A given intravenously significantly inhibited the development of Shay ulcers and indomethacin-induced gastric lesions in rats. It also inhibited water-immersion stress lesions induced in pylorus-ligated rats.

  17. Topical Aloe Vera (Aloe barbadensis Miller) Extract Does Not Accelerate the Oral Wound Healing in Rats.

    Science.gov (United States)

    Coelho, Fernanda Hack; Salvadori, Gabriela; Rados, Pantelis Varvaki; Magnusson, Alessandra; Danilevicz, Chris Krebs; Meurer, Luise; Martins, Manoela Domingues

    2015-07-01

    The effect of topical application of Aloe Vera (Aloe barbadensis Miller) extract was assessed on the healing of rat oral wounds in an in vivo model using 72 male Wistar rats divided into three groups (n = 24): control, placebo and Aloe Vera (0.5% extract hydroalcoholic). Traumatic ulcers were caused in the dorsum of the tongue using a 3-mm punch tool. The Aloe Vera and placebo group received two daily applications. The animals were sacrificed after 1, 5, 10 and 14 days. Clinical analysis (ulcer area and percentage of repair) and histopathological analysis (degree of re-epithelialization and inflammation) were performed. The comparison of the differences between scores based on group and experimental period, both in quantitative and semi-quantitative analyses, was performed using the Kruskal-Wallis test. The significance level was 5%. On day 1, all groups showed predominantly acute inflammatory infiltrate. On day 5, there was partial epithelialization and chronic inflammatory infiltrate. On the days 10 and 14 total repair of ulcers was observed. There was no significant difference between groups in the repair of mouth ulcers. It is concluded that treatment using Aloe Vera as an herbal formulation did not accelerate oral wound healing in rats. Copyright © 2015 John Wiley & Sons, Ltd.

  18. Prevention of the onset of hyperglycaemia by extracts of Aloe ...

    African Journals Online (AJOL)

    GREGO

    The ability of a home-made aqueous extract of Aloe barbadensis to prevent the onset of alloxan- induced hyperglycaemia was examined and compared with that of a factory-produced gel. Three groups of animals were administered 200 mg/kg body weight of alloxan intraperitoneally. A fourth group of animals was left ...

  19. Prevention of the onset of hyperglycaemia by extracts of Aloe ...

    African Journals Online (AJOL)

    The ability of a home-made aqueous extract of Aloe barbadensis to prevent the onset of alloxaninduced hyperglycaemia was examined and compared with that of a factory-produced gel. Three groups of animals were administered 200 mg/kg body weight of alloxan intraperitoneally. A fourth group of animals was left ...

  20. Consumption of aqueous extract of raw Aloe Vera leaves ...

    African Journals Online (AJOL)

    Forty-five juvenile tilapia and 30 weanling albino rats exposed to water containing 50, 100 and 150ppm of aqueous extract of Aloe Vera leaves for 96 hours and 28 days, respectively were used for this study. Fifteen tilapia and 10 rats exposed to clean water (0 ppm A. vera) served as controls. Clinical signs, mortality, gross ...

  1. The Effects of Crude Aqueous and Alcohol Extracts of Aloe Vera on ...

    African Journals Online (AJOL)

    The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day ...

  2. SYNTHESIS AND CHARACTERIZATION OF BIODEGRDABLE PLASTIC FROM CASAVA STARCH AND ALOE VERA EXTRACT WITH GLYCEROL PLASTICIZER

    OpenAIRE

    Mery Apriyani; Endaruji Sedyadi

    2016-01-01

    Synthesis and characterizations of Biodegradable Plastic made of Cassava Waste Starch, glycerol, acetic acid and Aloe vera extract has done. The aims of this research are to study the influence of addition of aloe vera extract in plastics mechanics properties, water vapor transmission rate and biodegradation. There are five main steps in this research, extraction of aloe vera, cassava starch preparation from cassava waste, preparations, characterization and biodegradability study of biodegrad...

  3. SYNTHESIS AND CHARACTERIZATION OF BIODEGRDABLE PLASTIC FROM CASAVA STARCH AND ALOE VERA EXTRACT WITH GLYCEROL PLASTICIZER

    National Research Council Canada - National Science Library

    Mery Apriyani; Endaruji Sedyadi

    2016-01-01

    .... There are five main steps in this research, extraction of aloe vera, cassava starch preparation from cassava waste, preparations, characterization and biodegradability study of biodegradable plastic...

  4. The efficacy of crude extract of Aloe secundiflora on Candida Albicans

    African Journals Online (AJOL)

    In- vitro studies on the efficacy of crude extracts of Aloe secundiflora on Candida albicans was conducted. Five mature leaves of Aloe secundiflora were collected and the crude extract was prepared, then autoclaved. The extract was then tested on Candida albicans grown on solid media. The results from these studies ...

  5. Antidiabetic effects of Aloe ferox and Aloe greatheadii var. davyana leaf gel extracts in a low-dose streptozotocin diabetes rat model

    Directory of Open Access Journals (Sweden)

    Lisa Botes

    2011-07-01

    Full Text Available The medicinal use and commercialisation of the plants Aloe ferox and Aloe greatheadii are primarily based on research done on Aloe vera and Aloe arborescens. Consequently, in this study we investigated the possible antidiabetic effects of ethanol extracts of A. ferox and A. greatheadii var. davyana leaf gel in a streptozotocin (STZ-induced type 2 diabetes rat model. Fifty male Wistar rats, weighing 200 g – 250 g, were randomly divided into five groups of n = 10: normal control rats, diabetic control rats, diabetic rats receiving A. ferox leaf gel extract (300 mg/kg, diabetic rats receiving A. greatheadii leaf gel extract (300 mg/kg, and diabetic rats receiving glibenclamide (600 μg/kg. Diabetes was induced by a single intraperitoneal injection of STZ (40 mg/kg. Rats were sacrificed 5 weeks after injection, following a 12-hour fast, and blood and tissue samples were collected. Compared to the normal control group, STZ significantly increased relative liver and kidney weights, end-point plasma glucose, fructosamine, oxidative stress, liver enzymes, total cholesterol (TC, triglycerides, very low density lipoprotein-cholesterol and TC: high density lipoprotein-cholesterol (HDL-C values and reduced serum insulin levels. Treatment with A. greatheadii moderately increased serum insulin and HDL-C levels and moderately reduced end-point plasma glucose and liver alkaline phosphatase (ALP and significantly decreased TC:HDL-C ratios. A. ferox supplementation similarly resulted in moderately increased serum insulin, accompanied by slight corrections in ALP and HDL-C, without any change to end-point plasma glucose values. A. greatheadii and, to a lesser extent, A. ferox, resulted in a clinically relevant improved diabetic state (indicated by moderate to high effect sizes, suggesting that these Aloe species may show promise

  6. Anticonvulsant activity of Aloe vera leaf extract in acute and chronic models of epilepsy in mice.

    Science.gov (United States)

    Rathor, Naveen; Arora, Tarun; Manocha, Sachin; Patil, Amol N; Mediratta, Pramod K; Sharma, Krishna K

    2014-03-01

    The effect of Aloe vera in epilepsy has not yet been explored. This study was done to explore the effect of aqueous extract of Aloe vera leaf powder on three acute and one chronic model of epilepsy. In acute study, aqueous extract of Aloe vera leaf (extract) powder was administered in doses 100, 200 and 400 mg/kg p.o. Dose of 400 mg/kg of Aloe vera leaf extract was chosen for chronic administration. Oxidative stress parameters viz. malondialdehyde (MDA) and reduced glutathione (GSH) were also estimated in brain of kindled animals. In acute study, Aloe vera leaf (extract) powder in a dose-dependent manner significantly decreased duration of tonic hind limb extension in maximal electroshock seizure model, increased seizure threshold current in increasing current electroshock seizure model, and increased latency to onset and decreased duration of clonic convulsion in pentylenetetrazole (PTZ) model as compared with control group. In chronic study, Aloe vera leaf (extract) powder prevented progression of kindling in PTZ-kindled mice. Aloe vera leaf (extract) powder 400 mg/kg p.o. also reduced brain levels of MDA and increased GSH levels as compared to the PTZ-kindled non-treated group. The results of study showed that Aloe vera leaf (extract) powder possessed significant anticonvulsant and anti-oxidant activity. © 2013 Royal Pharmaceutical Society.

  7. The effect of aqueous Aloe vera gel extract on serum mineral ...

    African Journals Online (AJOL)

    This study was conducted to determine the effect of Aloe vera gel extract on the serum mineral compositions of Red Sokoto bucks. Blood samples were collected from 30 bucks before the commencement of administration of Aloe vera gel extract for serum minerals and these served as control group. The bucks were now ...

  8. Effects of Aloe vera Extract on the Haemostatic Mechanism of Albino ...

    African Journals Online (AJOL)

    Plants and plant extracts have been found very useful for purposes of treatment of diseases among other benefits. Aloe vera is one such plants, whose effects on the haemostatic mechanism of albino wistar rats were studied. Results showed that Aloe vera did not increase the haemoglobin concentration, and packed cell ...

  9. Effect of Different Aloe Fractions on the Growth of Lactic Acid Bacteria.

    Science.gov (United States)

    Chiodelli, Giulia; Pellizzoni, Marco; Ruzickova, Gabriela; Lucini, Luigi

    2017-01-01

    Several foods on the market, such as yogurt and fermented milk, include mixtures of prebiotics and probiotic microorganisms effective in promoting the proliferation and equilibrium of intestinal bacteria, thus improving gut health. Particularly, researchers and the public have shown increasing interest in the combination of probiotics with natural substances that promote health or that can act as substrates to promote bacterial growth. The aim of this study is to evaluate the effects of different extracts of Aloe barbadensis and Aloe arborescens in fermented milk, taking into account both the prebiotic effect of aloe polysaccharides and the antimicrobial activity of several secondary metabolites. The results demonstrate a beneficial effect of 5% aloe inner gel on Lactobacillus growth and confirm the antimicrobial activity of the phenolic compounds peculiar of green rind extracts. © 2016 Institute of Food Technologists®.

  10. Mechanism of growth inhibitory effect of cape aloe extract in ehrlich ascites tumor cells.

    Science.gov (United States)

    Kametani, Saeda; Oikawa, Tomoko; Kojima-Yuasa, Akiko; Kennedy, David Opare; Norikura, Toshio; Honzawa, Mayumi; Matsui-Yuasa, Isao

    2007-12-01

    Cape aloe (Aloe ferox Miller) has been a herb well known for its cathartic properties and has also been used popularly as a health drink (juice, tea and tonic) in the United States and in Europe. Cape aloe extract also has been reported to possess several pharmacological effects, such as anti-inflammatory, anti-bacterial, anti-fungal and protective effect against liver injury. However, the investigations on an anti-tumor activity in cape aloe extract are very few and subsequent mechanisms have not been well elucidated. In this study, we examined the effect of the selective growth inhibitory activity of cape aloe extract and found that the cape aloe extract, especially the dichloromethane (CH(2)Cl(2)) extract, caused a dose-dependent growth inhibitory effect in Ehrlich ascites tumor cells (EATC), but not in mouse embryo fibroblast (NIH3T3) cells, which was used as a normal cell model. Furthermore, the CH(2)Cl(2) extract caused an accumulation of cells in the G1 phase and a decrease of cells in the S and G2/M phase of the cell cycle and inhibited DNA synthesis in a dose-dependent manner. In addition, other results suggest that cell cycle arrest and inhibition of proliferation in EATC by the CH(2)Cl(2 )extract are associated with decreased retinoblastoma protein (Rb) phosphorylation.

  11. Comparative study of antimicrobial activities of Aloe vera extracts ...

    African Journals Online (AJOL)

    Plants are of relevance to dermatology for both their beneficial and adverse effects on skin and skin disorders. One of the medicinal plants, Aloe vera (Aloe barbadensis Miller), is reputed to have medicinal properties. For centuries, it has been used for an array of ailments such as mild fever, wounds and burns, ...

  12. Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study.

    Science.gov (United States)

    Rezazadeh, Fahimeh; Moshaverinia, Maryam; Motamedifar, Mohammad; Alyaseri, Montazer

    2016-03-01

    Herpes simplex virus (HSV) infection is one of the most common and debilitating oral diseases; yet, there is no standard topical treatment to control it. The extract of Aloe vera leaves has been previously reported to have anti-inflammatory, antibacterial, and also antiviral effects. There is no data on anti-Herpes simplex virus type 1 (HSV-1) activity of Aloe vera gel. This study aimed to evaluate the anti-HSV-1 activity of Aloe vera gel in Vero cell line. In this study, gel extraction and cytotoxicity of various increasing concentrations of Aloe vera gel (0.2, 0.5, 1, 2, and 5%) was evaluated in Dulbecco's Modified Eagle Medium (DMEM) containing 2% fetal bovine serum (FBS). Having been washed with phosphate buffered saline, 50 plaque-forming units (PFU) of HSV-1 was added to each well. After 1 hour of incubation at 37°C, cell monolayers in 24 well plates were exposed to different increasing concentrations of Aloe vera gel. The anti-HSV-1 activity of Aloe vera gel in different concentrations was assessed by plaque reduction assays. Data were analyzed by using One-way ANOVA. The cytotoxicity assay showed that Aloe vera in prearranged concentrations was cell-compatible. The inhibitory effect of various concentrations of Aloe vera was observed one hour after the Vero cell was infected with HSV-1. However, there was no significant difference between two serial concentrations (p> 0.05). One-way ANOVA also revealed no significant difference between the groups. The findings indicated a dose-dependent antiviral effect of Aloe vera. The findings showed significant inhibitory effect of 0.2-5% Aloe vera gel on HSV-1 growth in Vero cell line. Therefore, this gel could be a useful topical treatment for oral HSV-1 infections without any significant toxicity.

  13. SYNTHESIS AND CHARACTERIZATION OF BIODEGRDABLE PLASTIC FROM CASAVA STARCH AND ALOE VERA EXTRACT WITH GLYCEROL PLASTICIZER

    Directory of Open Access Journals (Sweden)

    Mery Apriyani

    2016-05-01

    Full Text Available Synthesis and characterizations of Biodegradable Plastic made of Cassava Waste Starch, glycerol, acetic acid and Aloe vera extract has done. The aims of this research are to study the influence of addition of aloe vera extract in plastics mechanics properties, water vapor transmission rate and biodegradation. There are five main steps in this research, extraction of aloe vera, cassava starch preparation from cassava waste, preparations, characterization and biodegradability study of biodegradable plastic. The addition variations of aloe vera extract that used in this research are 0.01; 0.03; 0.05; 0.07 and 0.14 grams. Results showed that the addition of aloe Vera tends to increased biodegrable plastic thickness to 0.01 mm and elongation to 32.07%. However, biodegradable plastic tensile strength tends to decreased to 23.95 Mpa. Optimum tensile strength is 3.90 Mpa and elongation is 34.43%. Optimum water vapor transmission rate is 2.40 g/m2hours. Biodegradation study of biodegradable plastic showed that addition of aloe vera extract doesn’t significantly influence in plastic degradations.

  14. New flavonoids from bioactive extract of Algerian medicinal plant Launeae arborescens

    OpenAIRE

    Khaled Sekkoum; Nasser Belboukhari; Abdelkrim Cheriti

    2014-01-01

    Objective: To investigate the butanol fraction of the water/acetone extract and isolate of the new flavonoids from Launeae arboescens. Methods: The compounds were isolated by liquid chromatographic methods and their structures were identified by using spectroscopic analysis. Results: The isolated compounds were identified as: 7-O-[α-rhamnopyranosyl 4',5,6-Trihydroxy flavone 1,4′,5′-Di-Methoxy 7-(5′-Me Hexan)1-oyl flavanone 2, 3”-isopropyl pyrano [1”:7,4”:6] 3′,4′,5′,5-Tetrahydroxy flava...

  15. New flavonoids from bioactive extract of Algerian medicinal plant Launeae arborescens.

    Science.gov (United States)

    Sekkoum, Khaled; Belboukhari, Nasser; Cheriti, Abdelkrim

    2014-04-01

    To investigate the butanol fraction of the water/acetone extract and isolate of the new flavonoids from Launeae arboescens. The compounds were isolated by liquid chromatographic methods and their structures were identified by using spectroscopic analysis. THE ISOLATED COMPOUNDS WERE IDENTIFIED AS: 7-O-[α-rhamnopyranosyl 4',5,6-Trihydroxy flavone 1,4',5'-Di-Methoxy 7-(5″-Me Hexan)1-oyl flavanone 2, 3″-isopropyl pyrano [1″:7,4″:6] 3',4',5',5-Tetrahydroxy flavanone 3,5,4',5'-Tri-Hydroxy 7-(3″-Me butan) -yl flavanone 4, 5,7-Dihydroxy-2',4',5' -trimethoxy-isoflavanone 5,5,6,7,4'-tetrahydroxy flavonol 6,7-O-[α-rhamnopyranosyl-(1->6)-β-glucopyranosyl]- 4',5,7-tri-hydroxy-flavanone 7,7-O-[α-rhamnopyranosyl-(1->6)-β-glucopyranosyl] 3',5-Dihydroxy 4'-Methoxy flavanone 8. The presence of different types of bioactive flavonoids in Launeae arboescens extract can explain the large ethnopharmacological uses and the potential activity of this medicinal plant.

  16. New flavonoids from bioactive extract of Algerian medicinal plant Launeae arborescens

    Science.gov (United States)

    Sekkoum, Khaled; Belboukhari, Nasser; Cheriti, Abdelkrim

    2014-01-01

    Objective To investigate the butanol fraction of the water/acetone extract and isolate of the new flavonoids from Launeae arboescens. Methods The compounds were isolated by liquid chromatographic methods and their structures were identified by using spectroscopic analysis. Results The isolated compounds were identified as: 7-O-[α-rhamnopyranosyl 4′,5,6-Trihydroxy flavone 1,4′,5′-Di-Methoxy 7-(5″-Me Hexan)1-oyl flavanone 2, 3″-isopropyl pyrano [1″:7,4″:6] 3′,4′,5′,5-Tetrahydroxy flavanone 3,5,4′,5′-Tri-Hydroxy 7-(3″-Me butan) -yl flavanone 4, 5,7-Dihydroxy-2′,4′,5′ –trimethoxy-isoflavanone 5,5,6,7,4′-tetrahydroxy flavonol 6,7-O-[α-rhamnopyranosyl-(1->6)-β-glucopyranosyl]- 4′,5,7-tri-hydroxy-flavanone 7,7-O-[α-rhamnopyranosyl-(1->6)-β-glucopyranosyl] 3′,5-Dihydroxy 4′-Methoxy flavanone 8. Conclusions The presence of different types of bioactive flavonoids in Launeae arboescens extract can explain the large ethnopharmacological uses and the potential activity of this medicinal plant. PMID:25182549

  17. [Bacteriostatic and/or bactericidal extract of Aloe vera gel on cultures of Listeria monocytogenes].

    Science.gov (United States)

    Ramírez Mérida, Luis Guillermo; Morón de Salim, Alba; Catinella, Rosangela; Castillo, Luis

    2012-03-01

    Listeria monocytogenes is a bacteria responsible for food borne diseases (FBD). The effect of Aloe vera gel extract as a possible bacteriostatic and/or bactericidal against Listeria monocytogenes, was checked by determined the minimum inhibitory concentration (MIC), the time of minimum inhibition (TMI) and minimum bactericidal concentration (MBC) solutions extract of Aloe vera gel in different concentrations on cultures of Listeria monocytogenes ATCC 7635. We applied the agar diffusion method, using solutions of extract of Aloe vera gel at concentrations of 0 to 100% for the MIC. The TMI was determined by growth curves in trypticase soy broth with an initial inoculum of Listeria monocytogenes ATCC 7635 of 108 CFU/mL in each solution. It was determined that the MIC was 10% extract of Aloe vera gel and TMI was 5 hours at concentrations of 10%, 20% and 30% of Aloe vera, while concentrations of 50, 80, 90 and 100%, the time was 8 hours. It was found that indeed the Aloe vera gel is bacteriostatic power on Listeria monocytogenes (p < 0.001), but yet, no bactericidal effect was obtained in our study.

  18. Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Cho, Jae Young; Chung, Byung Yeoup [Chonbuk National University, Jeonju (Korea, Republic of)

    2011-10-15

    The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, {beta}-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

  19. Reproductive potential of male catfish treated with gel extract of Aloe ...

    African Journals Online (AJOL)

    The reproductive potential of male catfish treated with gel extract of Aloe vera plant was studied using twelve male fish weighing 500-560g.The fish were divided into 3 groups; A, B and C with four fish in each group. Group A was treated with 2% Aloe vera gel while group B was treated with 3% and Group C the control was ...

  20. Holistic Healing Through Herbs: Effectiveness of Aloe Vera on Post Extraction Socket Healing.

    Science.gov (United States)

    Nimma, Vijaya Lakshmi; Talla, Harsha Vardhan; Bairi, Jaya Krishna; Gopaldas, Madhulatha; Bathula, Haritha; Vangdoth, Sandeep

    2017-03-01

    Advances in the field of alternative medicine has encouraged the use of various natural products for multiple uses in the field of dentistry for treatment of various oral diseases. A natural herbal product is Aloe vera, which has number of benefits with no reported side effects and gaining considerable importance in clinical research. The aim of this cross-sectional randomized interventional study was to evaluate the effectiveness of Aloe vera in the healing of post extraction sockets. A sample size of 40 patients undergoing atraumatic extraction was divided into two groups. Group A-patients were prescribed only analgesics and followed for seven consecutive days and socket healing assessment was done. Group B-Patients were given Aloe vera soaked gel foams, followed up to the third and seventh day by two observers and the socket healing was assessed using the standardized index by Landry, Turnbull and Howley. In addition to healing the common complaint associated with extraction wound, the pain was assessed using numerical rating scale. Data obtained was statistically analysed using Mann-whitney U test, Wilcoxon Signed ranks test and Spearman's rank correlation method. Control group on the third and the seventh day showed healing of 60% and 70% respectively and the Aloe vera group showed a better result having a healing potential of 70% on the third day and 90% on the seventh day which was statistically significant with a (p-valueAloe vera group showed a significant decrease in pain after two hours on the day of extraction followed by second, third and seventh day which was statistically significant (p-valueAloe vera has been proved to have a unique property that is implicated in better healing than other group without any side effects. Aloe vera is economical, effective, powerful nutritional supplement and antioxidant that protects and promotes wound healing.

  1. The Use of Aloe Vera Extract as a Novel Storage Media for the Avulsed Tooth

    Directory of Open Access Journals (Sweden)

    Samaneh Badakhsh

    2014-07-01

    Full Text Available Background: Tooth avulsion is one of the most severe dental traumas which most often occur in children. When immediate replantation is not possible, storage in a proper media may lead to a prolonged survival rate. Aloe Vera is a cactus like plant with green, tapered leaves that are filled with a transparent viscous gel. This medicinal plant has significant anti-inflammatory, antioxidant, antibacterial and antifungal effects. The purpose of this study was to assess the effectiveness of different concentrations of Aloe Vera extract compared to DMEM (cell culture medium and egg white. Methods: The periodontal ligament (PDL cells were cultured and certain number of cells were treated with Aloe Vera extract (in four different concentrations, egg white and culture media for 1, 3, 6, and 9 hours. Cell viability was determined by using the (3-[4, 5-dimethylthiazolyl-2]-2, 5-diphenyltetrazolium bromide assay. Moreover, One-way ANOVA and post hoc (LSD test were used for analyzing the study groups. Results: The results indicate that culture media and Aloe Vera extract (10, 30, and 50% concentration were statistically similar and significantly preserved more PDL cells compared to other experimental storage media. Conclusion: Aloe Vera 10, 30, and 50% may be recommended as a suitable storage media for avulsed teeth.

  2. The use of aloe vera extract as a novel storage media for the avulsed tooth.

    Science.gov (United States)

    Badakhsh, Samaneh; Eskandarian, Tahereh; Esmaeilpour, Tahereh

    2014-07-01

    Tooth avulsion is one of the most severe dental traumas which most often occur in children. When immediate replantation is not possible, storage in a proper media may lead to a prolonged survival rate. Aloe Vera is a cactus like plant with green, tapered leaves that are filled with a transparent viscous gel. This medicinal plant has significant anti-inflammatory, antioxidant, antibacterial and antifungal effects. The purpose of this study was to assess the effectiveness of different concentrations of Aloe Vera extract compared to DMEM (cell culture medium) and egg white. The periodontal ligament (PDL) cells were cultured and certain number of cells were treated with Aloe Vera extract (in four different concentrations), egg white and culture media for 1, 3, 6, and 9 hours. Cell viability was determined by using the (3-[4, 5-dimethylthiazolyl-2]-2, 5-diphenyltetrazolium bromide) assay. Moreover, One-way ANOVA and post hoc (LSD) test were used for analyzing the study groups. The results indicate that culture media and Aloe Vera extract (10, 30, and 50% concentration) were statistically similar and significantly preserved more PDL cells compared to other experimental storage media. Aloe Vera 10, 30, and 50% may be recommended as a suitable storage media for avulsed teeth.

  3. Dye-sensitized solar cells using Aloe Vera and Cladode of Cactus extracts as natural sensitizers

    Science.gov (United States)

    Ganta, D.; Jara, J.; Villanueva, R.

    2017-07-01

    The purpose of this study is to develop dye-sensitized solar cells (DSSCs) from natural plant-based dyes, extracted from the Cladode (nopal) of the Thornless Prickly Pear Cactus (Opuntia ficus-indica), the gel of Aloe Vera (Aloe barbadensis miller), and the combination of Cladode and Aloe Vera extracts on side-by-side configuration. Optical properties were analyzed using UV-Vis Absorption and Fourier Transform Infrared Spectroscopy. Open circuit voltages (Voc) varied from 0.440 to 0.676 V, fill factors (FF) were greater than 40%, short-circuit photocurrent densities (Jsc) ranged from 0.112 to 0.290 mA/cm2 and highest conversion efficiency of 0.740% was reported for the Cladode DSSC.

  4. The effects of crude aqueous and alcohol extracts of Aloe vera on growth and abdominal viscera of suckling rats.

    Science.gov (United States)

    Beya, Wabeya; Davidson, Bruce; Erlwanger, Kennedy H

    2012-01-01

    The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day old Sprague-Dawley rats were gavaged with aqueous or alcohol extracts of Aloe vera (low dose 50mg. kg⁻¹ or high dose 500mg. kg⁻¹) daily for eight days. All data were expressed as mean ± SD and analyzed by one way ANOVA. Pups receiving high doses of either extract had a significantly higher body mass gain than the group receiving lower dose (p Aloe vera extracts resulted in growth promotion, enhanced hepatic storage of metabolic substrates, increased ALP possibly in relation to bone growth and caused hypertrophy of the caecum of neonatal rats. These effects need to be explored further to enhance animal production and health.

  5. The Genus Aloe: Phytochemistry and Therapeutic Uses Including Treatments for Gastrointestinal Conditions and Chronic Inflammation.

    Science.gov (United States)

    Cock, I E

    2015-01-01

    Plants of the genus Aloe have perhaps the longest recorded history of medicinal usage and are amongst the most widely used plants for traditional medicinal purposes worldwide. Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, but many other species are also used for their therapeutic properties. The Aloes have been used since ancient times, particularly for the treatment of microbial infections, gastrointestinal disorders and inflammatory conditions. In addition to their myriad uses in traditional therapeutics, the Aloes have also been used as components of cosmetic formulations, and in the food and beverage industries. Despite their wide acceptance, studies from different laboratories often report wide variations in the therapeutic bioactivities from within the same Aloe species, even when the same extraction procedures are used. Furthermore, leaves from individual Aloe plants within the same species may have widely varying levels of the bioactive phytochemicals. Phytochemical analyses have shown that many Aloe species contain various carbohydrate polymers (notably glucomannans) and a range of other low molecular weight phenolic compounds including alkaloids, anthraquinones, anthrones, benzene and furan derivatives, chromones, coumarins, flavonoids, phytosterols, pyrans and pyrones. There has been a wealth of information published about the phytochemistry and therapeutic potential of the Aloes (especially Aloe vera). Much of this has been contradictory. Intra- and interspecies differences in the redox state of the individual Aloe components and in the ratios of these components may occur between individual plants. These factors may all affect the physiological properties of Aloe extracts. Due to the structure and chemical nature of many of the Aloe phytochemicals, it is likely that many of the reported medicinal properties are due to antioxidant or prooxidant effects. The antioxidant/prooxidant activities of many Aloe

  6. The effect of Aloe vera extract on humoral and cellular immune ...

    African Journals Online (AJOL)

    tonukari sunday

    2011-06-13

    Jun 13, 2011 ... Some plant polysaccharides are well known to possess immunostimulatory effects. Aloe vera possesses confirmed curative or healing actions. The aim of this study was to evaluate the effect of the administration of A. vera plant extract on cellular and humoral immune response in rabbits. 20 healthy.

  7. The effect of Aloe vera extract on humoral and cellular immune ...

    African Journals Online (AJOL)

    Some plant polysaccharides are well known to possess immunostimulatory effects. Aloe vera possesses confirmed curative or healing actions. The aim of this study was to evaluate the effect of the administration of A. vera plant extract on cellular and humoral immune response in rabbits. 20 healthy male New Zealand white ...

  8. Anti-Sickling Potential of Aloe Vea Extract Iii: Gas Chromatography ...

    African Journals Online (AJOL)

    The neutral metabolite obtained from ethanol extract of Aloe vera was characterized using the Gas Chromatography/Mass Spectroscopy. The results showed that the oil contained five different compounds, which included hexadecanoic acid, ethyl ester; phytol; 2-methyloctadeca-3,13-diene-1-ol; cotadeca-9,12,15-trienoic ...

  9. In vitro activity of Allium sativum and Aloe vera extract against ...

    African Journals Online (AJOL)

    The rapid increase of antibiotic resistance needs to be taken as a threat to both animals and human being. In this study the arbitrary concentration of 25%, 50% and 75% of individual and combined Allium sativum and Aloe vera were tested against Salmonella Gallinarum. The antimicrobials were extracted using aqueous ...

  10. Mechanism of Aloe Vera extract protection against UVA: shelter of lysosomal membrane avoids photodamage.

    Science.gov (United States)

    Rodrigues, Daniela; Viotto, Ana Cláudia; Checchia, Robert; Gomide, Andreza; Severino, Divinomar; Itri, Rosangela; Baptista, Maurício S; Martins, Waleska Kerllen

    2016-03-01

    The premature aging (photoaging) of skin characterized by wrinkles, a leathery texture and mottled pigmentation is a well-documented consequence of exposure to sunlight. UVA is an important risk factor for human cancer also associated with induction of inflammation, immunosuppression, photoaging and melanogenesis. Although herbal compounds are commonly used as photoprotectants against the harmful effects of UVA, the mechanisms involved in the photodamage are not precisely known. In this study, we investigated the effects of Aloe Vera (Aloe barbadensis mil) on the protection against UVA-modulated cell killing of HaCaT keratinocytes. Aloe Vera exhibited the remarkable ability of reducing both in vitro and in vivo photodamage, even though it does not have anti-radical properties. Interestingly, the protection conferred by Aloe Vera was associated with the maintenance of membrane integrity in both mimetic membranes and intracellular organelles. The increased lysosomal stability led to a decrease in lipofuscinogenesis and cell death. This study explains why Aloe Vera extracts offer protection against photodamage at a cellular level in both the UV and visible spectra, leading to its beneficial use as a supplement in protective dermatological formulations.

  11. Aloe vera Aqueous Extract Effect on Morphine Withdrawal Syndrome in Morphine-Dependent Female Rats.

    Science.gov (United States)

    Shahraki, Mohammad Reza; Mirshekari, Hamideh; Sabri, Azame

    2014-09-01

    Aloe vera is a medicinal herb used as an anti-inflammatory and sedative agent. The current study aimed to evaluate the effect of Aloe vera aqueous extract on morphine withdrawal symptoms in morphine-dependent female rats. The current research was performed on 40 female Wista-Albino rats which were made dependent on morphine using Houshyar protocol and were randomly divided into five groups (A, B, C, D, and E). Group A did not receive any agent in the period of handling but other groups (B, C, D and E) received 5, 10, 20 and 40 mg/kg of Aloe vera aqueous extract by gavage, three times daily for a week, respectively. Withdrawal symptoms, stool form, agitation, disparity, floppy eyelids, and body mass variations were checked for 10 days. The obtained data were analyzed using SPSS v.11 software, and Friedman, Kruskal-Wallis, and Mann-Whitney statistical tests. Statistical difference was considered significant (P Aloe vera aqueous extract had various effects on morphine withdrawal syndrome in morphine-dependent female rats .

  12. Acemannan, an extracted polysaccharide from Aloe vera: A literature review.

    Science.gov (United States)

    Sierra-García, Gerardo Daniel; Castro-Ríos, Rocío; González-Horta, Azucena; Lara-Arias, Jorge; Chávez-Montes, Abelardo

    2014-08-01

    In this review, the composition, actions, and clinical applications of acemannan in medicine and its effectiveness as an adjunct in the treatment of diseases are presented. An electronic literature search was performed up to January 2014 for studies and research presenting data to validate the efficacy of acemannan. A total of 50 titles, abstracts and full-text studies were selected and reviewed. Acemannan has various medicinal properties like osteogenic, anti-inflammatory, and antibacterial, which accelerate healing of lesions. Also, acemannan is known to have antiviral and antitumor activities in vivo through activation of immune responses. It was concluded that Aloe vera has immense potential as a therapeutic agent. Even though the plant is a promising herb with various clinical applications in medicine and dentistry, more clinical research needs to be undertaken to validate and explain the action of acemannan in healing, so that it can be established in the field of medicine and a more precise understanding of the biological activities of these is required to develop Aloe vera as a pharmaceutical source.

  13. The ultra-structural survey of Aloe Vera extract on photoreceptor layer after methanol intoxication

    Directory of Open Access Journals (Sweden)

    M Bahramian

    2015-12-01

    Full Text Available Background & aim: The photoreceptor layer is a main part of the retina. The Aloe Vera has antioxidant, antibacterial and antidiabetic effects. The aim of this study was to investigate the ultra-structure of the photoreceptor layer of male rats under the effect of methanol intoxication and protective effects of Aloe Vera extract against the methanol toxicity. Methods: The present experimental study was conducted on 30 adult male wistar rats in three groups of ten: as control, methanol intoxication, methanol intoxication receiving 400 mg/kg Aloe Vera extract for 30 days. Methanol intoxication was induced by intraperitoneal injection of 4gr/kg of methanol for 30 days. At the end of experiment, the eyes were removed and retina was separated near the optic disc and immersed in gluteraldehyde 4%. The retinal tissue was rinsed with buffer and fixed in osmium tetroxide 1% and dehydrated through a graded alcohol series. Then, the tissues were placed in a mixture of propylene oxide and resin and embedded in pure resin. Semi thin and ultrathin sections prepared and studied by transmission electron and light microscopes. Using One Way ANOVA and Tukey test data were analyzed. Result: Results showed that revealed decrement of the pathological sign in Aloe Vera-methanol group in comparison to methanol intoxication group. The outer segment loss, highly vacuolization in inner segment and condense and pyknotic nuclei were seen in methanol intoxication group. But the moderate outer segment loss, lightly vacuolization in inner segment and condense nuclei were observed in Aloe Vera-methanol group. Morphometric observations showed that the thickness of photoreceptor layer decreased in methanol intoxication group and little reduction in Aloe Vera-methanol group. The decrement of thickness in this group has significant difference with control group. But the decrement of thickness of photoreceptor layer in Aloe Vera-methanol group has no significant difference with

  14. Comparison of healing effect of aloe vera extract and silver sulfadiazine in burn injuries in experimental rat model.

    Science.gov (United States)

    Akhoondinasab, Mohammad Reza; Akhoondinasab, Motahhare; Saberi, Mohsen

    2014-01-01

    Wound healing is widely discussed in the medical literature. This study compared the healing effect of aloe vera extract and silver sulfadiazine in burn injuries in experimental rat model. Sixteen rats were randomly assigned to one of two groups, each group 8 rats. A deep second-degree burn on the lower back and 3(rd) degree burn on upper back of each rat were created with a standard burning procedure. Burns were dressed daily with aloe vera extract in group 2 and silver sulfadiazine in group 1. Response to treatment was assessed by digital photography during treatment until day 32. Histological parameters (PMN, epithelialization, fibrosis and angiogenesis) were assessed after biopsy of scar at the end of research. Wound healing was more visible in aloe vera group. Also the speed of healing in aloe vera group was better than silver sulfadiazine group. Based on our findings, aloe vera can be a therapy of choice for burn injuries.

  15. Mosquito larvicidal activity of Aloe vera (Family: Liliaceae) leaf extract and Bacillus sphaericus, against Chikungunya vector, Aedes aegypti

    National Research Council Canada - National Science Library

    Subramaniam, Jayapal; Kovendan, Kalimuthu; Mahesh Kumar, Palanisamy; Murugan, Kadarkarai; Walton, William

    2012-01-01

    The bio-efficacy of Aloe vera leaf extract and bacterial insecticide, Bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of Aedes aegypti, under the laboratory conditions...

  16. Anticoccidial effects of Aloe secundiflora leaf extract against Eimeria tenella in broiler chicken.

    Science.gov (United States)

    Kaingu, Fredrick; Liu, Dandan; Wang, Lele; Tao, Jianping; Waihenya, Rebecca; Kutima, Helen

    2017-04-01

    Anticoccidial effects of Aloe secundiflora crude leaf extract was tested in broiler chickens following oral infection with Eimeria tenella. Sixty 22-day-old birds were divided into six groups of ten birds each. Three treatment groups A, B, and C were fed with the extract (100, 250, and 500 mg/day, respectively) mixed in feed for 10 days, and three control groups: group D (drug control) administered 300 mg/l of sulfachloropyrazine sodium soluble powder in drinking water for 5 days, group E (infected/non-medicated positive control), and group F (uninfected/non-medicated negative control). Except for group F, all groups were orally inoculated with 75,000 sporulated oocysts of E. tenella. The effects of the extract on E. tenella infection were evaluated by severity of bloody diarrhea, body weight (BW) gain, oocyst output, and lesion score. No bird in the treated groups died of coccidiosis, and severity of bloody diarrhea was milder than in the positive control group. BW gains in the treated groups were significantly higher than in group E (p Aloe dosage. The findings of this study suggest that Aloe secundiflora extract presents an alternative anticoccidial agent for the control of avian coccidiosis.

  17. Aloe Vera Extract Effect on Sperm Quality and Testicular Tissue of Rats Induced by Cadmium Chloride

    Directory of Open Access Journals (Sweden)

    F Farhangdoost

    2014-04-01

    Full Text Available Background & aim: A lot of physical and chemical factors cause infertility disorders. Cadmium is a chemical agent which damages the cell structure of the reproductive system. For reducing the effects of various factors, new traditional methods have been used. The aim of this study was to investigate the effects of Aloe vera extract on testicular tissue of rats induced by cadmium chloride. Methods: In this experimental study, 40 male Wistar rats (180-200 gr were randomly divided into four groups. Groups 1 and 2 received Cadmium chloride (1/5 mg / kg/ IP. Mice induced by cadmium chloride were treated with Aloe vera. Control and normal rats were treated with 400 mg/kg of Aloe vera extracts. After 25 days, these rats were weighed and then anesthetized using ether. Blood samples were collected from each individual to assess the level of testosterone and then the animals were debriefed. The testes were removed and transferred to 10% formalin solution. After tissue processing, 5 micron sections were prepared and stained with heamatoxillin-eosin and investigated by light microscope. Data were analyzed by one-way ANOVA test. Results: Mean seminiferous tubular diameter, number of spermatogonia, Leydig and Sertoli cell of cadmium control group compared to the healthy control group showed a significant decrease (p<0.05. The mean sperm count and sperm motility in extract cadmium group and healthy control group was close to normal and displayed a significant difference (p< 0.05. Conclusion: Hydroalcoholic extract of Aloe vera increases the number of spermatogonia, Leydig and Sertoli testicular tissue of mice contaminated with cadmium chloride

  18. A comparison of the leaf gel extracts of Aloe ferox and Aloe vera in the topical treatment of atopic dermatitis in Balb/c mice.

    Science.gov (United States)

    Finberg, M J; Muntingh, G L; van Rensburg, C E J

    2015-12-01

    Aloe vera gel is widely used in the treatment of an array of disturbances, especially skin disorders. The wound-healing effects have been attributed to its moisturizing and anti-inflammatory effects as well as its beneficial effect on the maturation of collagen. The aim of the present study is to compare the effects of topically applied extracts of Aloe ferox with that of Aloe vera on the symptoms as well as IgE levels of a mouse model of atopic dermatitis (AD). Mice were sensitized and challenged with 2,4-dinitrochlorobenzene and treated afterwards for 10 consecutive days with the gels of either A. ferox or A. vera applied topically to the affected areas. A placebo gel was used for the control mice. Blood was collected at the beginning and end of the treatment period to measure serum IgE levels. Although the gels of both the Aloe species inhibited the cutaneous inflammatory response as well as serum IgE levels in the rats, the extracts of A. ferox were superior to that of A. vera in reducing IgE levels. The gels of A. ferox and A. vera, applied topically, may be a safe and useful alternative to antihistamines and topical corticosteroids, for the treatment of patients suffering from recurring chronic AD.

  19. Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis.

    Science.gov (United States)

    Dotta, G; Ledic-Neto, J; Gonçalves, E L T; Brum, A; Maraschin, M; Martins, M L

    2015-05-01

    This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.

  20. Clear Evidence of Carcinogenic Activity by a Whole-Leaf Extract of Aloe barbadensis Miller (Aloe vera) in F344/N Rats

    Science.gov (United States)

    Boudreau, Mary D.

    2013-01-01

    Aloe barbadensis Miller (Aloe vera) is an herbal remedy promoted to treat a variety of illnesses; however, only limited data are available on the safety of this dietary supplement. Drinking water exposure of F344/N rats and B6C3F1 mice to an Aloe vera whole-leaf extract (1, 2, and 3%) for 13 weeks resulted in goblet cell hyperplasia of the large intestine in both species. Based upon this observation, 2-year drinking water studies were conducted to assess the carcinogenic potential of an Aloe vera whole-leaf extract when administered to F344/N rats (48 per sex per group) at 0.5, 1, and 1.5%, and B6C3F1 mice (48 per sex per group) at 1, 2, and 3%. Compared with controls, survival was decreased in the 1.5% dose group of female rats. Treatment-related neoplasms and nonneoplastic lesions in both species were confined primarily to the large intestine. Incidences of adenomas and/or carcinomas of the ileo-cecal and cecal-colic junction, cecum, and ascending and transverse colon were significantly higher than controls in male and female rats in the 1 and 1.5% dose groups. There were no neoplasms of the large intestine in mice or in the 0 or 0.5% dose groups of rats. Increased incidences of mucosa hyperplasia of the large intestine were observed in F344/N rats, and increased incidences of goblet cell hyperplasia of the large intestine occurred in B6C3F1 mice. These results indicate that Aloe vera whole-leaf extract is an intestinal irritant in F344/N rats and B6C3F1 mice and a carcinogen of the large intestine in F344/N rats. PMID:22968693

  1. Clear evidence of carcinogenic activity by a whole-leaf extract of Aloe barbadensis miller (aloe vera) in F344/N rats.

    Science.gov (United States)

    Boudreau, Mary D; Mellick, Paul W; Olson, Greg R; Felton, Robert P; Thorn, Brett T; Beland, Frederick A

    2013-01-01

    Aloe barbadensis Miller (Aloe vera) is an herbal remedy promoted to treat a variety of illnesses; however, only limited data are available on the safety of this dietary supplement. Drinking water exposure of F344/N rats and B6C3F1 mice to an Aloe vera whole-leaf extract (1, 2, and 3%) for 13 weeks resulted in goblet cell hyperplasia of the large intestine in both species. Based upon this observation, 2-year drinking water studies were conducted to assess the carcinogenic potential of an Aloe vera whole-leaf extract when administered to F344/N rats (48 per sex per group) at 0.5, 1, and 1.5%, and B6C3F1 mice (48 per sex per group) at 1, 2, and 3%. Compared with controls, survival was decreased in the 1.5% dose group of female rats. Treatment-related neoplasms and nonneoplastic lesions in both species were confined primarily to the large intestine. Incidences of adenomas and/or carcinomas of the ileo-cecal and cecal-colic junction, cecum, and ascending and transverse colon were significantly higher than controls in male and female rats in the 1 and 1.5% dose groups. There were no neoplasms of the large intestine in mice or in the 0 or 0.5% dose groups of rats. Increased incidences of mucosa hyperplasia of the large intestine were observed in F344/N rats, and increased incidences of goblet cell hyperplasia of the large intestine occurred in B6C3F1 mice. These results indicate that Aloe vera whole-leaf extract is an intestinal irritant in F344/N rats and B6C3F1 mice and a carcinogen of the large intestine in F344/N rats.

  2. Effects of dietary Aloe vera crude extracts on digestive enzyme ...

    African Journals Online (AJOL)

    Gabriel Gabriel

    2017-10-16

    Oct 16, 2017 ... El-Dakar (2015) reported that basil (Ocimum basilicum) extracts significantly increased serum lipase activity in Sparus aurata fingerlings, while protease activity was reported to increase significantly in. Labeo rohita fingerlings after being fed with Mucuna pruriens ethanolic extracts (Ojha et al., 2014).

  3. Extracts from Aloe ferox and Withania somnifera inhibit Candida ...

    African Journals Online (AJOL)

    Withania somnifera are among the southern African plants commonly used for the treatment of (STIs). This paper reports on the in vitro antimicrobial activities of water and methanol extracts from the two plants on Neisseria gonorrhoea and Candida albicans, common causes of STIs in rural South Africa. Extracts from both ...

  4. Toxicology and carcinogenesis studies of a nondecolorized [corrected] whole leaf extract of Aloe barbadensis Miller (Aloe vera) in F344/N rats and B6C3F1 mice (drinking water study).

    Science.gov (United States)

    Boudreau, M D; Beland, F A; Nichols, J A; Pogribna, M

    2013-08-01

    Extracts from the leaves of the Aloe vera plant (Aloe barbadensis Miller) have long been used as herbal remedies and are also now promoted as a dietary supplement, in liquid tonics, powders or tablets, as a laxative and to prevent a variety of illnesses. We studied the effects of Aloe vera extract on rats and mice to identify potential toxic or cancer-related hazards. We gave solutions of nondecolorized extracts of Aloe vera leaves in the drinking water to groups of rats and mice for 2 years. Groups of 48 rats received solutions containing 0.5%, 1% or 1.5% of Aloe vera extract in the drinking water, and groups of mice received solutions containing 1%, 2%, or 3% of Aloe vera extract. Similar groups of animals were given plain drinking water and served as the control groups. At the end of the study tissues from more than 40 sites were examined for every animal. In all groups of rats and mice receiving the Aloe vera extract, the rates of hyperplasia in the large intestine were markedly increased compared to the control animals. There were also increases in hyperplasia in the small intestine in rats receiving the Aloe vera extract, increases in hyperplasia of the stomach in male and female rats and female mice receiving the Aloe vera extract, and increases in hyperplasia of the mesenteric lymph nodes in male and female rats and male mice receiving the Aloe vera extract. In addition, cancers of the large intestine occurred in male and female rats given the Aloe vera extract, though none had been seen in the control groups of rats for this and other studies at this laboratory. We conclude that nondecolorized Aloe vera caused cancers of the large intestine in male and female rats and also caused hyperplasia of the large intestine, small intestine, stomach, and lymph nodes in male and female rats. Aloe vera extract also caused hyperplasia of the large intestine in male and female mice and hyperplasia of the mesenteric lymph node in male mice and hyperplasia of the stomach

  5. Semen characteristics and sperm morphological studies of the West African Dwarf Buck treated with Aloe vera gel extract

    Directory of Open Access Journals (Sweden)

    Oyeyemi Matthew Olugbenga

    2011-01-01

    Full Text Available Background: Aloe vera (Aloe barbadensis miller is an evergreen perennial plant widely used in modern herbal practice and is often available in proprietary herbal preparations.Objective: This study was designed to investigate the semen picture and spermatozoa morphology of West African Dwarf (WAD bucks treated with Aloe vera extract.Materials and Methods: Twelve sexually matured WAD bucks, weighing between 11 and 15 kg, were used for the study. The bucks were first used as control (pre-treatment and later as two groups of six animals each. The first six bucks received 10 mls of the 3% extract while the other six received 10 mls of the 4% of the extract for a 7 day period. Semen was collected from both the 3 and 4% extract treated bucks for the control (pre-treatment, on days eight (first week post-treatment and fifteen (second week post-treatment in each case using the electroejaculation method. The spermiogram of the bucks were investigated using standard procedures. Data obtained were analyzed using two way ANOVA and significance reported at p<0.05.Results: The continuous administration of Aloe vera extract significantly (p<0.05 reduced sperm concentration, motility and percentage livability and resulted in increased sperm abnormalities in the WAD buck.Conclusion: Aloe vera adversely affected the spermiogram of bucks. The plant can reduce fertility in male animals and is therefore not recommended for medicinal purpose in male animals especially those used for breeding.

  6. Semen characteristics and sperm morphological studies of the West African Dwarf Buck treated with Aloe vera gel extract

    Science.gov (United States)

    Olugbenga, Oyeyemi Matthew; Olukole, Samuel Gbadebo; Adeoye, Ajayi Tolulope; Adejoke, Adeniji Deborah

    2011-01-01

    Background: Aloe vera (Aloe barbadensis miller) is an evergreen perennial plant widely used in modern herbal practice and is often available in proprietary herbal preparations. Objective: This study was designed to investigate the semen picture and spermatozoa morphology of West African Dwarf (WAD) bucks treated with Aloe vera extract. Materials and Methods: Twelve sexually matured WAD bucks, weighing between 11 and 15 kg, were used for the study. The bucks were first used as control (pre-treatment) and later as two groups of six animals each. The first six bucks received 10 mls of the 3% extract while the other six received 10 mls of the 4% of the extract for a 7 day period. Semen was collected from both the 3 and 4% extract treated bucks for the control (pre-treatment), on days eight (first week post-treatment) and fifteen (second week post-treatment) in each case using the electroejaculation method. The spermiogram of the bucks were investigated using standard procedures. Data obtained were analyzed using two way ANOVA and significance reported at p<0.05. Results: The continuous administration of Aloe vera extract significantly (p<0.05) reduced sperm concentration, motility and percentage livability and resulted in increased sperm abnormalities in the WAD buck. Conclusion: Aloe vera adversely affected the spermiogram of bucks. The plant can reduce fertility in male animals and is therefore not recommended for medicinal purpose in male animals especially those used for breeding. PMID:25587252

  7. Effectiveness of the aqueous extract of aloe in the treatment of oral alveolitis

    Directory of Open Access Journals (Sweden)

    Isidro de Jesús Nápoles González

    2015-07-01

    Full Text Available Background: alveolitis is a complication that may appear after the extraction of a tooth. It is considered a dental emergency due to the intense pain. Its prevalence is worrisome for the dental services for the complications and disability that it can provoke in patients.Objective: to evaluate the effectiveness of the aqueous extract of aloe with pharmaceutical use in the treatment of alveolitis.Method: an experimental study was carried out with 100 patients that visited the Provincial Teaching Dental Clinic, “La Vigía” Clinic, and “Julio Antonio Mella” Polycilinic of Camaguey in 2014, and that were diagnosed with alveolitis. The sample was made up of 50 patients in the control group treated with Alvogyl and 50 patients in the study group treated with aloe.Results: most of the patients belonged to the 26 to 45 age group. At 24 hours of treatment the results on pain relief were similar for both groups, with an 18 % for the study group and a 16 % for the control group. At 48 hours the best results were found in the control group, with 62 % of patients with no pain, and only a 30 % in the study group. At 72 hours this tendency was kept with 90 % in the control group and 72 % in the study group. In the study group an 84 % of the patients with wet alveolitis felt pain relief.Conclusions: the aqueous extract of aloe is a natural drug which is effective in the treatment of alveolitis, mainly in wet alveolitis and those affecting the maxillary bones. There were no adverse reactions with none of the used medications.

  8. Hypoglycemic and Hypolipidemic Effects of Aloe vera Extract Preparations: A Review.

    Science.gov (United States)

    Pothuraju, Ramesh; Sharma, Raj Kumar; Onteru, Suneel Kumar; Singh, Satvinder; Hussain, Shaik Abdul

    2016-02-01

    Obesity is considered to be an epidemic disease, and it is associated with several metabolic disorders. Pharmacological treatments currently available are not effective for prolonged treatment duration. So, people are looking toward new therapeutic approach such as herbal ingredients. Since ancient periods, different herbs have been used for remedy purposes such as anti-obesity, antidiabetes, and antiinflammatory. Among the several herbal ingredients, Aloe vera (Aloe barbadensis Miller) is widely used to curb the metabolic complications. Till date, reports are not available for the side effects of A. vera. Several researchers are used to different solvents such as aqueous solution, alcohol, ethanol, and chloroform for the A. vera extract preparations and studied their hypoglycemic and hypolipidemic effects in animal and human studies. Furthermore, little information was recorded with the active compounds extracted from the A. vera and their anti-obesity and antidiabetic effects in clinical studies. In this review, we made an attempt to compile all the available literature by using different search engines (PubMed, Scopus, and Google Scholar) on the A. vera extract preparations and the possible mechanism of action involved in carbohydrate and lipid metabolism. Copyright © 2015 John Wiley & Sons, Ltd.

  9. Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques.

    Science.gov (United States)

    Dal'Belo, Susi Elaine; Gaspar, Lorena Rigo; Maia Campos, Patrícia Maria Berardo Gonçalves

    2006-11-01

    The polysaccharide-rich composition of Aloe vera extracts (Aloe barbadensis Miller), often used in cosmetic formulations, may impart moisturizing properties to the product. The aim of this study was to evaluate the effect of cosmetic formulations containing different concentrations of freeze-dried Aloe vera extract on skin hydration, after a single and a 1- and 2-week period of application, by using skin bioengineering techniques. Stable formulations containing 5% (w/w) of a trilaureth-4 phosphate-based blend were supplemented with 0.10%, 0.25% or 0.50% (w/w) of freeze-dried Aloe vera extract and applied to the volar forearm of 20 female subjects. Skin conditions in terms of the water content of the stratum corneum and of transepidermal water loss (TEWL) (Corneometer CM 825 and Tewameter TM 210) were analysed before and after a single and 1- and 2-week period of daily application. After a single application, only formulations supplemented with 0.25% and 0.50% (w/w) of Aloe vera extract increased the water content of the stratum corneum, while after the 2-week period application, all formulations containing the extract (0.10%, 0.25% and 0.50%) had the same effect, in both cases as compared with the vehicle. TEWL was not modified after a single and after 1- and 2-week period of application, when compared with the vehicle. Our results show that freeze-dried Aloe vera extract is a natural effective ingredient for improving skin hydration, possibly through a humectant mechanism. Consequently, it may be used in moisturizing cosmetic formulations and also as a complement in the treatment of dry skin.

  10. [The therapeutic properties of aloe].

    Science.gov (United States)

    Iena, Ia M

    1993-01-01

    Data are reported on the use of aloes in research and folk medicine. Aloes is used in the form of dry juice of aloe leaves, fluid extract, juice, ointments. The author discusses indications and contraindications to the use of aloe. Recipes are given of mixtures with aloe which may be used in domestic conditions for increasing the defensive forces of the body during radiation lesions.

  11. Microwave-assisted fibrous decoration of mPE surface utilizing Aloe vera extract for tissue engineering applications.

    Science.gov (United States)

    Balaji, Arunpandian; Jaganathan, Saravana Kumar; Supriyanto, Eko; Muhamad, Ida Idayu; Khudzari, Ahmad Zahran Md

    2015-01-01

    Developing multifaceted, biocompatible, artificial implants for tissue engineering is a growing field of research. In recent times, several works have been reported about the utilization of biomolecules in combination with synthetic materials to achieve this process. Accordingly, in this study, the ability of an extract obtained from Aloe vera, a commonly used medicinal plant in influencing the biocompatibility of artificial material, is scrutinized using metallocene polyethylene (mPE). The process of coating dense fibrous Aloe vera extract on the surface of mPE was carried out using microwaves. Then, several physicochemical and blood compatibility characterization experiments were performed to disclose the effects of corresponding surface modification. The Fourier transform infrared spectrum showed characteristic vibrations of several active constituents available in Aloe vera and exhibited peak shifts at far infrared regions due to aloe-based mineral deposition. Meanwhile, the contact angle analysis demonstrated a drastic increase in wettability of coated samples, which confirmed the presence of active components on glazed mPE surface. Moreover, the bio-mimic structure of Aloe vera fibers and the influence of microwaves in enhancing the coating characteristics were also meticulously displayed through scanning electron microscopy micrographs and Hirox 3D images. The existence of nanoscale roughness was interpreted through high-resolution profiles obtained from atomic force microscopy. And the extent of variations in irregularities was delineated by measuring average roughness. Aloe vera-induced enrichment in the hemocompatible properties of mPE was established by carrying out in vitro tests such as activated partial thromboplastin time, prothrombin time, platelet adhesion, and hemolysis assay. In conclusion, the Aloe vera-glazed mPE substrate was inferred to attain desirable properties required for multifaceted biomedical implants.

  12. Enhanced biocompatibility of ZnS:Mn quantum dots encapsulated with Aloe vera extract for therapeutic applications

    Science.gov (United States)

    Anilkumar, M.; Bindu, K. R.; Sneha Saj, A.; Anila, E. I.

    2016-08-01

    Toxicity of nanoparticles remains to be a major issue in their application to the biomedical field. Aloe vera (AV) is one of the most widely exploited medicinal plants that have a multitude of amazing properties in the field of medicine. Methanol extract of Aloe vera can be used as a novel stabilising agent for quantum dots to reduce toxicity. We report the synthesis, structural characterization, antibacterial activity and cytotoxicity studies of ZnS:Mn quantum dots synthesized by the colloidal precipitation method, using methanol extract of Aloe vera (AVME) as the capping agent. The ZnS:Mn quantum dots capped with AVME exhibit superior performances in biocompatibility and antibacterial activity compared with ZnS:Mn quantum dots without encapsulation. Project supported by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India.

  13. Aloe vera extract as a promising treatment for the quality maintenance of minimally-processed table grapes

    Directory of Open Access Journals (Sweden)

    Giuseppina Rosaria Antonella Alberio

    2015-06-01

    Full Text Available The effect of an edible film obtained from a commercial Aloe vera extract, on the quality maintenance of minimally processed grapes belonging to three different cultivars (Sugar One, Victoria and Black Magic was evaluated by enzymatic (PPO, PME, β-GAL, physicochemical (pH, acidity, °Brix, and sensorial methods. All the analyzed parameters were measured in extracts obtained from minimally processed grapes packaged in ordinary atmosphere and stored at 4 °C for 15 days. Samples dipped into Aloe vera showed significant differences (p≤0.05 compared to untreated ones. The determination of such parameters and the evaluation of consumer acceptability were helpful to determine the effectiveness of the post-harvest treatment with Aloe vera for a storage period of 15 days.

  14. Comparison of Healing Effect of Aloe Vera Extract and Silver Sulfadiazine in Burn Injuries in Experimental Rat Model

    Science.gov (United States)

    Akhoondinasab, Mohammad Reza; Akhoondinasab, Motahhare; Saberi, Mohsen

    2014-01-01

    BACKGROUND Wound healing is widely discussed in the medical literature. This study compared the healing effect of aloe vera extract and silver sulfadiazine in burn injuries in experimental rat model. METHODS Sixteen rats were randomly assigned to one of two groups, each group 8 rats. A deep second-degree burn on the lower back and 3rd degree burn on upper back of each rat were created with a standard burning procedure. Burns were dressed daily with aloe vera extract in group 2 and silver sulfadiazine in group 1. Response to treatment was assessed by digital photography during treatment until day 32. Histological parameters (PMN, epithelialization, fibrosis and angiogenesis) were assessed after biopsy of scar at the end of research. RESULTS Wound healing was more visible in aloe vera group. Also the speed of healing in aloe vera group was better than silver sulfadiazine group. CONCLUSIONS Based on our findings, aloe vera can be a therapy of choice for burn injuries. PMID:25489521

  15. Phytochemical Contents and Antioxidant Capacities of Two Aloe greatheadii var. davyana Extracts

    Directory of Open Access Journals (Sweden)

    Du Toit Loots

    2008-09-01

    Full Text Available Aloe greatheadii var. davyana (Asphodelaceae is used among rural South African communities to treat arthritis, skin cancer, burns, eczema, psoriasis, digestive problems, high blood pressure and diabetes, despite very little supporting scientific evidence. Due to increased interest by both the scientific community and industry regarding the medicinal uses of this plant species, we identified, quantified and compared the phytochemical contents and antioxidant capacities of two extracts of A. greatheadii; a leaf gel extract (LGE and a 95 % aqueous ethanol leaf gel extract (ELGE, using various modified extraction procedures, GC-MS and spectrophotometry. Apart from extensively characterizing this medicinal plant with regards to its organic acid, polyphenols/phenolic acid, alcohol, aldehyde, ketone, alkane, pyrimidine, indole, alkaloid, phytosterol, fatty acid and dicarboxylic acid contents and antioxidant capacities, we describe a modified extraction procedure for the purpose of general phytochemical characterization, and compare this to a 95 % aqueous ethanol extraction technique. From the results it is clear that A. greatheadii contains a variety of compounds with confirmed antioxidant capacity and other putative health benefits (such as blood glucose, cholesterol and cortisol lowering properties relating to the prevention or treatment of diabetes, cardiovascular disease, cancer and hypertension. The results also indicate that separate ethyl acetate/diethyl ether and hexane extractions of the LGE, better serve for general phytochemical characterization purposes, and 95 % aqueous ethanol extraction for concentrating selective groups of health related compounds, hence justifying its use for biological in vivo efficacy studies.

  16. Antifungal effect of Malaysian ;Aloe vera' leaf extract on selected fungal species of pathogenic otomycosis species in in vitro culture medium

    National Research Council Canada - National Science Library

    Saniasiaya, Jeyasakthy; Salim, Rosdan; Mohamad, Irfan; Harun, Azian

    2017-01-01

    ...: This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian 'Aloe vera' leaf was prepared in ethanol and solutions via the Soxhlet extraction method...

  17. Mosquitocidal and water purification properties of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus leaf extracts.

    Science.gov (United States)

    Ethanolic extracts of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus were tested for toxicity to 3rd instar Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti. Median lethal concentrations (LC50) were, respectively, 0.44%, 0.51%, 0.59% and 0.68%. Cynodon dactylon...

  18. Caragana arborescens Lam.: Siberian peashrub

    Science.gov (United States)

    Donald R. Dietz; Paul E. Slabaugh; Franklin T. Bonner

    2008-01-01

    Siberian peashrub - Caragana arborescens Lam. - is one of the most hardy small deciduous trees or shrubs planted on the northern Great Plains (George 1953; Rehder 1940). Introduced into North America in 1752 (Rehder 1940), Siberian peashrub is native to Siberia and Manchuria and occurs from southern Russia to China (Graham 1941). Varieties include the dwarf (C. a. nana...

  19. Encapsulation of Aloe Vera extract into natural Tragacanth Gum as a novel green wound healing product.

    Science.gov (United States)

    Ghayempour, Soraya; Montazer, Majid; Mahmoudi Rad, Mahnaz

    2016-12-01

    Application of natural materials in wound healing is an interest topic due to effective treatment with no side effects. In this paper, Aloe Vera extract was encapsulated into Tragacanth Gum through a sonochemical microemulsion process to prepare a wound healing product. FESEM/EDX and FT-IR proved the successfully formation of the nanocapsules with spherical shape by cross-linking aluminum ions with Tragacanth Gum. The therapeutic characteristics of the prepared wound healing product were investigated using antimicrobial, cytotoxicity and wound healing assays. Relative high antimicrobial activities with the microbial reduction of 84, 91 and 80% against E. coli, S. aureus and C. albicans, a cell viability of 98% against human fibroblast cells and a good wound healing activity with considerable migration rate of fibroblast cells are the important advantages of the new formed wound healing product. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. A novel topical association with zinc oxide, chamomile and aloe vera extracts - stability and safety studies

    Directory of Open Access Journals (Sweden)

    Catarina Reis

    2015-12-01

    Full Text Available Currently, natural products show an enormous potential for pharmaceutical and cosmetic industries. The goals of this study were to formulate and to characterise a novel combination of natural products. Formulations were 1 an oil-in-water emulsion, 2 a water-in-oil emulsion and 3 a cleansing solution. Zinc oxide was chosen as an active ingredient due to its healing properties, and chamomile and aloe vera extracts were chosen due to their antiseptic, anti-inflammatory and tissue regenerating properties. Organoleptic characteristics, pH, viscosity and in vitro efficacy for the most common bacteria and yeast of human skin were evaluated. Preliminary and accelerated stability studies and safety tests were also performed. All optimized products were stable, smooth in texture, effective against bacteria and yeast, and safe, justifying further studies. Results suggest that these novel products might be a promising source of natural compounds with soothing and regenerative properties for skin care.

  1. The analysis of different processes of extraction: yield of extracts obtained from Aloe Vera (Aloe barbadensis Miller and Sweet Bay (Laurus nobilis L. and the exergy analysis of applied processes

    Directory of Open Access Journals (Sweden)

    Jasna Ivanović

    2009-10-01

    Full Text Available The present study was aimed to investigate and compare the efficiency of different methods for the isolation extract from a plant material. Extracts from dried leaves of Aloe vera (A. barbadensis Mill. and Sweet Bay (Laurus nobilis L. were obtained applying the following techniques: supercritical carbon dioxide (SC CO2 extraction at 30 MPa and 100 °C, a combined ultrasonic extraction and pre-treatment using SC CO2 (US-SCCO2, and a conventional ultrasonic extraction (US with 96% ethanol. US-SCCO2 resulted in much higher yields of extracts of aloe and bay compared to SC CO2 extraction. Determined extract’s yield was used for calculating the maximum useful work and exergy loss as a measure of irreversibility of the extraction process. Calculated maximum useful work per 1 kg of the extract was ranged from 0.7 to 226 kJ while estimated values of the exergy loss were from 48 to 416 kJ per 1 kg of the extract. The maximum useful work and exergy loss have the largest value for SCE much more than for other processes of extraction (US and US-SCCO2. The pre-treatment of the plant material with SC CO2 before an ultrasound-assisted extraction or the decrease of a particle size used for ultrasound-assisted extraction of Aloe vera has led to the decrease of maximum useful work and exergy loss. Unlike, pre-treatment with SC CO2 followed by ultrasound-assisted extraction increased the exergy loss when bay was used as the plant material.

  2. Antimutagenicity and Anticarcinogenic Effects of Gel and Latex Extracts of Aloe Vera Cultivated: A Comparative Study in Two Cities, Iran

    Directory of Open Access Journals (Sweden)

    Kheiri A

    2012-03-01

    Full Text Available Background: Nowadays, cancer is one of the main causes of mortality in the world and many mutagens are the cause of death in millions of patients. Due to the side effects of anticancer drugs, scientists are in search of natural drugs with fewer side effects and more therapeutic efficacy. This study aims to, firstly, investigate the antimutgenic effects of different Aloe vera gel and latex extracts on mutated Salmonella typhimurium bacterium by using Ames test and to, secondly, study the probable effects of the habitat conditions on the antimutagenic effects of the plant.Methods: After preparing different Aloe vera gel and latex extracts, the antimutagenic effects of the extracts were evaluated by Ames test. In this test, a mutated strain of S. typhimurium was grown on culture media containing a minimum of salt and glucose in the presence of a mutagen substance (NaN3. Subsequently, only those bacteria that had turned HIS+ by reverse mutation formed colonies. As different alcoholic and aqueous extracts of Aloe vera reduced reversed mutations, the difference between the means of revertant mutants per plate was calculated by one-way ANOVA using SPSS software (version 18. Results: The ethanol extracts of latex from Karaj had a maximum (91% and aqueous extract from Dezfoul had a minimum (42% percentage of inhibition. Conclusion: Maximum percentage of inhibition was observed in the extracts of the plant cultivated in Karaj reflecting the impact of environmental conditions on the construction of antioxidant compounds in plants.

  3. Magnetic nanoporous carbon as an adsorbent for the extraction of phthalate esters in environmental water and aloe juice samples.

    Science.gov (United States)

    Liu, Li; Hao, Yunhui; Ren, Yiqian; Wang, Chun; Wu, Qiuhua; Wang, Zhi

    2015-05-01

    In this work, magnetic nanoporous carbon with high surface area and ordered structure was synthesized using cheap commercial silica gel as template and sucrose as the carbon source. The prepared magnetic nanoporous carbon was firstly used as an adsorbent for the extraction of phthalate esters, including diethyl phthalate, diallyl phthalate, and di-n-propyl-phthalate, from lake water and aloe juice samples. Several parameters that could affect the extraction efficiency were optimized. Under the optimum conditions, the limit of detection of the method (S/N = 3) was 0.10 ng/mL for water sample and 0.20 ng/mL for aloe juice sample. The linearity was observed over the concentration range of 0.50-150.0 and 1.0-200.0 ng/mL for water and aloe juice samples, respectively. The results showed that the magnetic nanoporous carbon has a high adsorptive capability toward the target phthalate esters in water and aloe juice samples. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. The Anti-Nociceptive Effect of Aloe. Vera Aqueous Extract in Fructose-Fed Male Rats

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    Mohammad Reza Shahraki

    2010-05-01

    Full Text Available A B S T R A C T Introduction: Aloe Vera extract is used as an anti-inflammatory and anti-bradikinin agent in laboratory animals. The aim of this survey was to evaluate the ant-nociceptive effect of A. Vera aqueous extract in fructose-fed male rats. Methods: Forty-five Wistar-Albino male rats were equally and randomly divided into five groups including sham operated and four test groups. Sham operated group consumed tap water and the test groups consumed fructoseenriched water. Test groups 2, 3 and 4 additionally received, 0, 100, 150 and 200 mg/kg of A. Vera extract, respectively, whereas the other test group received distilled water daily. Tail flick reaction time, serum glucose and oral glucose tolerance test (OGTT were measured. The results were analyzed by SPSS software using ANOVA and Tukey tests. Results were expressed as mean ± SD. Statistical differences were considered significant at p<0.05. Results: The results showed that tail flick reaction time significantly increased in test group 3 which received 200 mg/kg A. Vera extract comparing with that of sham operated group. However, OGTT and serum glucose value were significantly increased in all fructose-fed male rats comparing with those of sham operated group. Discussion: These results indicated that A. Vera aqueous extract can affect tail flick reaction time in fructose-fed male rats. Further studies are required to show the exact mechanism of anti-nociceptive effect of A. Vera extract.

  5. Prevention of tri-nitrobenzene of sulfonic acid-induced colitis in chicken by using extract of Aloe vera

    Directory of Open Access Journals (Sweden)

    Motamed Elsayed Mahmoud

    Full Text Available Aim: Aloe vera, species of succulent plant in the genus Aloe, has multiple clinical activities and used routinely to accelerate wound healing. The present study was designed to investigate the anti-inflammatory effect of Aloe vera extracts (AVE in vitro and in vivo. Materials and Methods: The effect of crude AVE on inducible nitric oxide production by LPS/IFNg-stimulated cultured macrophages was evaluated. The therapeutic effect of administering crude Aloe vera extracts (100 mg/kg b.w. on the development of tri-nitrobenzene of sulfonic acid (TNBS-induced colitis (40 mg/kg b. w. in chicken was also investigated. Chicken is a valuable model for this purpose because it showed preference to bitter taste of Aloe vera. Diverse clinical pictures of the colitis including weight loss, diarrhea and histopathological changes were evaluated. Results: Nitrite production by LPS/IFNg-stimulated macrophages was maximally reduced by adding of AVE (100 μg/ml. This result suggests a direct inhibitory effect of AVE on the inflammatory cells. Chicks treated orally with AVE showed improvement of the histological signs with no inflammatory cell infiltrates and reduction of myeloperoxidase (MPO activities when compared with colitis control group. AVE pretreatment ameliorated significantly the clinical and histopathological severity of the TNBS-induced colitis; decreased body weight loss and diarrhea and increased survival. Conclusion: It was concluded that oral administration of AVE represents a valuable therapeutic approach for the treatment of colitis in chicken. [Vet. World 2012; 5(8.000: 469-476

  6. Mosquito larvicidal activity of Aloe vera (Family: Liliaceae) leaf extract and Bacillus sphaericus, against Chikungunya vector, Aedes aegypti

    OpenAIRE

    Subramaniam, Jayapal; Kovendan, Kalimuthu; Mahesh Kumar, Palanisamy; Murugan, Kadarkarai; Walton, William

    2012-01-01

    The bio-efficacy of Aloe vera leaf extract and bacterial insecticide, Bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of Aedes aegypti, under the laboratory conditions. The plant material was shade dried at room temperature and powdered coarsely. A. vera and B. sphaericus show varied degrees of larvicidal activity against various instars larvae of A. aegypti. The LC50 of A. vera against the first to fourth instars larvae were 162.74, 201.43, 253...

  7. Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity.

    Science.gov (United States)

    Logaranjan, Kaliyaperumal; Raiza, Anasdass Jaculin; Gopinath, Subash C B; Chen, Yeng; Pandian, Kannaiyan

    2016-12-01

    Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses and further supported by surface-enhanced Raman spectroscopy/Raman scattering (SERS) study. UV-visible spectrum has shown a sharp peak at 420 nm and further evidenced by FTIR peak profile (at 1587.6, 1386.4, and 1076 cm -1 with corresponding compounds). The main band position with SERS was noticed at 1594 cm -1 (C-C stretching vibration). When samples were heated under microwave radiation, AgNP with octahedron shapes with 5-50 nm were found and this method can be one of the easier ways to synthesis anisotropic AgNP, in which the plant extract plays a vital role to regulate the size and shape of the nanoparticles. Enhanced antibacterial effects (two- to fourfold) were observed in the case of Aloe vera plant protected AgNP than the routinely synthesized antibiotic drugs. Shape and size-controlled synthesis of silver nanoparticles using Aloe vera plant extract.

  8. Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium

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    Jeyasakthy Saniasiaya

    2017-01-01

    Full Text Available Objectives: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian Aloe vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans. Methods: This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC. Results: For A. niger, a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts of Aloe vera leaf for C. albicans. The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone of inhibition was obtained for C. albicans the MIC was not determined. Conclusions: The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the aqueous extract for A. niger (p < 0.001. Malaysian Aloe vera has a significant antifungal effect towards A. niger.

  9. Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium.

    Science.gov (United States)

    Saniasiaya, Jeyasakthy; Salim, Rosdan; Mohamad, Irfan; Harun, Azian

    2017-01-01

    Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian Aloe vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans. This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC). For A. niger, a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts of Aloe vera leaf for C. albicans. The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone of inhibition was obtained for C. albicans the MIC was not determined. The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the aqueous extract for A. niger (p Malaysian Aloe vera has a significant antifungal effect towards A. niger.

  10. Plasma-assisted deposition of microcapsule containing Aloe vera extract for cosmeto-textiles

    Science.gov (United States)

    Nascimento do Carmo, S.; Zille, A.; Souto, A. P.

    2017-10-01

    Dielectric Barrier Discharge (DBD) atmospheric-pressure plasma was employed to enhance the deposition of commercial microcapsules (MCs) containing Aloe vera extract onto a cotton/polyester (50:50) fabric. DBD conditions were optimized in term of energy dosage and contact angle. The MCs were applied by padding and printing methods and the coatings were characterized in terms of SEM and FTIR. MCs display a spherical shape with size between 2 and 8 μm with an average wall thickness of 0.5 μm. The MCs applied by printing and pretreated with a plasma dosage of 1.6 kW m2 min-1 showed the best results with an increased adhesion of 200% and significant penetration of MCs into the fibres network. Plasma printed fabric retained 230% more MCs than untreated fabric after 10 washing cycles. However, the coating resistance between unwashed and washed samples was only improved by 5%. Considering the fact that no binder or crosslinking agents were used, the DBD plasma-assisted deposition of MCs revealed to be a promising environmental safe and low cost coating technology.

  11. Lipoma arborescens: diagnosis and image; Lipoma arborescens: diagnostico e imagem

    Energy Technology Data Exchange (ETDEWEB)

    Goncalves, Marcela; Len, Claudio Arnaldo; Terreri, Maria Teresa Ramos Ascencao [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Setor de Reumatologia Pediatrica; Fernandes, Artur da Rocha Correa [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. de Diagnostico por Imagem; Hilario, Maria Odete Esteves [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Alergia, Imunologia e Reumatologia]. E-mail: odetehilario@terra.com.br

    2004-08-01

    Lipoma arborescens is an intraarticular lesion of unknown etiology, consisting of a chronic villous fat proliferation of the synovial membrane. The disease has occasionally been associated with diabetes mellitus, degenerative diseases, juvenile rheumatoid arthritis and also rheumatoid arthritis. The diagnosis relies on magnetic resonance imaging evaluation and synovial biopsy. We report a case of a 8-year-old girl with a two year history of bilateral swelling of the knees and elbows. The patient had improvement of the arthritis after starting treatment with conventional drugs. (author)

  12. Evaluation of wound healing treated with latex derived from rubber trees and Aloe Vera extract in rats.

    Science.gov (United States)

    Brandão, Marcelo Luiz; Reis, Paulo Roberto Melo; Araújo, Lilhian Alves de; Araújo, Ana Carolina Vicente; Santos, Maisa Helena de Abreu Silva; Miguel, Marina Pacheco

    2016-09-01

    To compare the use of latex derivative and Aloe vera extract to wound healing. Twenty one rats were randomly divided into three groups and each one had a wound made by incision. The treatment consisted in: derivative of latex (GL), Aloe vera extract (GA) and saline solution (GC). The wound area was measured on the 7th, 14th and 21st days and macroscopic and microscopic evaluation were done. The comparison between the measurements of the wounds presented statistical difference in GC and GA from the 7th day of evaluation and GL from the 14th day. The extent of the wound was significantly smaller by the 7th day in GL. Histologically, in GL, the neovascularization was significant on the 7th, 14th and 21st days. On the 21st day the scar was large and little mature. In GA and GC, the findings were similar on the 7th, 14th and 21st days with a slight better organization of skin and collagen on the 21st in GA. Statistical analysis did not allow for the definition of the best topical agent. The latex had the highest angiogenesis, but a possible foreign body granuloma. Aloe vera has revealed a healing process adequated temporally in histology.

  13. Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress

    Directory of Open Access Journals (Sweden)

    Fábio Sabbadin Zanuzzo

    2012-10-01

    Full Text Available This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus, to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS only at 0.1, 0.2, 0.5 and 1 mg/L. Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested.

  14. [Ultracytochemical studies of aloin in Aloe arborescens leaves].

    Science.gov (United States)

    Liao, Hai Min; Shen, Zong Gen; Sheng, Xian Yong; Hu, Zheng Hai

    2006-02-01

    Lead acetate precipitation method was used for ultracytochemical localization of aloin. The processes of aloin production, transport and storage were studied by transmission electron microscope. Results showed that aloin was produced in the plastids of the assimilating tissue. The aloin was transported through the plastid membrane to the surrounding endoplasmic reticulum and enveloped in the vesicles by the endoplasmic reticulum elements, the vesicles approached and later fused with the plasmalemma. Some vesicles of the plastid membrane directly fused with the plasmalemma. The vesicles released their contents into the apoplast through exocytosis, and finally reached the vascular bundle sheath by apoplastic translocation. Aloin was transported to the internal tangential wall of vascular bundle sheath cell through endoplasmic reticulum vesicles, and reached the cytoplasm of aloin cell by means of plasmodesmata. Finally, aloin was stored in the vacuoles of aloin cell.

  15. Preliminary studies on the efficacy of aloe vera ( Aloe barbadensis ...

    African Journals Online (AJOL)

    Extracts of Aloe vera were administered to experimentally infected mice with the Nigeria strain of Trypanosoma brucei brucei. The thirty-two (32) mice involved in the study were split into eight (8) groups of 4 mice each and treated intraperitoneally with the aqueous and ethanolic Aloe vera extracts of 40, 80, 20 mg/Kg body ...

  16. Assessment of the safety of aqueous extract of Aloe vera on ...

    African Journals Online (AJOL)

    Aghomotsegin

    2015-07-29

    Jul 29, 2015 ... Aloe vera is used both traditionally and packaged commercially in many regions of the world for several medicinal and or cosmetic purposes. It is claimed to have rejuvenating, moisturizing, healing or soothing properties on the skin and gastrointestinal tract. This study focused on assessment of the safety of ...

  17. Assessment of the Safety of Aqueous Extract of Aloe vera on ...

    African Journals Online (AJOL)

    Aloe vera is used both traditionally and packaged commercially in many regions of the world for several medicinal and or cosmetic purposes. It is claimed to have rejuvenating, moisturizing, healing or soothing properties on the skin and gastrointestinal tract. This study focused on assessment of the safety of A. vera on blood ...

  18. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

    Science.gov (United States)

    Lee, Yun Sun; Ju, Hyun Kyoung; Kim, Yeon Jeong; Lim, Tae-Gyu; Uddin, Md Romij; Kim, Yeon Bok; Baek, Jin Hong; Kwon, Sung Won; Lee, Ki Won; Seo, Hak Soo; Park, Sang Un; Yang, Tae-Jin

    2013-01-01

    Aloe vera (Asphodeloideae) is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  19. Dietary aloe vera gel powder and extract inhibit azoxymethane- induced colorectal aberrant crypt foci in mice fed a high- fat diet.

    Science.gov (United States)

    Chihara, Takeshi; Shimpo, Kan; Kaneko, Takaaki; Beppu, Hidehiko; Higashiguchi, Takashi; Sonoda, Shigeru; Tanaka, Miyuki; Yamada, Muneo; Abe, Fumiaki

    2015-01-01

    Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

  20. Enhancement of Anti-Inflammatory Activity of Aloe vera Adventitious Root Extracts through the Alteration of Primary and Secondary Metabolites via Salicylic Acid Elicitation

    Science.gov (United States)

    Lee, Yun Sun; Ju, Hyun Kyoung; Kim, Yeon Jeong; Lim, Tae-Gyu; Uddin, Md Romij; Kim, Yeon Bok; Baek, Jin Hong; Kwon, Sung Won; Lee, Ki Won; Seo, Hak Soo; Park, Sang Un; Yang, Tae-Jin

    2013-01-01

    Aloe vera (Asphodeloideae) is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10–11 and 5–13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment. PMID:24358188

  1. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

    Directory of Open Access Journals (Sweden)

    Yun Sun Lee

    Full Text Available Aloe vera (Asphodeloideae is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  2. An evaluation of the impact of aloe vera and licorice extracts on the course of experimental pigeon paramyxovirus type 1 infection in pigeons.

    Science.gov (United States)

    Dziewulska, D; Stenzel, T; Smialek, M; Tykalowski, B; Koncicki, A

    2018-02-01

    The progressive decrease in the efficiency of synthetic drugs has prompted research into phytogenic feed additives with potentially immunomodulatory and anti-infective properties. Complex diseases with a mixed etiology, including viral, pose a growing problem in domestic pigeons. The aim of this study was to determine the effectiveness of various doses of aloe vera and licorice extracts on the course of experimental PPMV-1 infection in pigeons. The experiment was performed on pigeons divided into 5 groups, including one control group and 4 experimental groups, which were orally administered aloe vera or licorice extracts at 300 or 500 mg/kg BW for 7 d after experimental inoculation with PPMV-1. On d 4, 7, and 14 after inoculation, cloacal swabs and samples of organs were collected from 4 birds in each group. The samples were analyzed to determine the copy number of PPMV-1 RNA by TaqMan qPCR. The results indicate that licorice and aloe vera extracts inhibited PPMV-1 replication by decreasing viral RNA copy numbers in the examined organs. The most inhibitory effect was observed in pigeons receiving aloe vera extract at 300 mg/kg BW, for which PPMV-1 RNA copy numbers were approximately 7-fold lower (brain), 9-fold lower (kidneys), and 14-fold lower (liver) than in the control group. The results of this study point to the potentially antiviral effects of aloe vera and licorice extracts in pigeons infected with PPMV-1. To the best of our knowledge, this is the first study to investigate the antiviral properties of aloe vera and licorice extracts in domestic pigeons. © The Author 2017. Published by Oxford University Press on behalf of Poultry Science Association.

  3. Effect of Gelatin-Based Edible Coatings Incorporated with Aloe vera and Black and Green Tea Extracts on the Shelf Life of Fresh-Cut Oranges

    Directory of Open Access Journals (Sweden)

    Mohsen Radi

    2017-01-01

    Full Text Available The aim of this study was to evaluate the effect of gelatin coating incorporated with Aloe vera gel (50,100% and green and black tea extracts (5,10% on physicochemical, microbial, and sensorial properties of fresh-cut oranges at 4°C for 17 days. Significant differences in terms of quality parameters were observed between the control and coated fresh-cut oranges. The highest variation of quality parameters was observed in control, while the least variations were observed in coated slices with 100% Aloe vera and 10% green tea extract. The weight loss was increased with time, but the coating treatment especially with 100% Aloe vera had significant effect on the prevention of weight loss. Also, Aloe vera coated samples obtained the highest score in sensory evaluation. Coating with gelatin incorporated with Aloe vera and green tea extracts successfully retarded the microbial growth and therefore extended the shelf life of fresh-cut oranges during cold storage.

  4. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

    Directory of Open Access Journals (Sweden)

    Patcharaporn Tippayawat

    2016-10-01

    Full Text Available Background There is worldwide interest in silver nanoparticles (AgNPs synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV with antibacterial activity. Methods AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively

  5. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity.

    Science.gov (United States)

    Tippayawat, Patcharaporn; Phromviyo, Nutthakritta; Boueroy, Parichart; Chompoosor, Apiwat

    2016-01-01

    There is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity. AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in pharmaceutical, biotechnological

  6. Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2014-03-01

    Full Text Available Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb. As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan

  7. Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity

    Science.gov (United States)

    Logaranjan, Kaliyaperumal; Raiza, Anasdass Jaculin; Gopinath, Subash C. B.; Chen, Yeng; Pandian, Kannaiyan

    2016-11-01

    Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses and further supported by surface-enhanced Raman spectroscopy/Raman scattering (SERS) study. UV-visible spectrum has shown a sharp peak at 420 nm and further evidenced by FTIR peak profile (at 1587.6, 1386.4, and 1076 cm-1 with corresponding compounds). The main band position with SERS was noticed at 1594 cm-1 (C-C stretching vibration). When samples were heated under microwave radiation, AgNP with octahedron shapes with 5-50 nm were found and this method can be one of the easier ways to synthesis anisotropic AgNP, in which the plant extract plays a vital role to regulate the size and shape of the nanoparticles. Enhanced antibacterial effects (two- to fourfold) were observed in the case of Aloe vera plant protected AgNP than the routinely synthesized antibiotic drugs.

  8. Aloe Vera for Tissue Engineering Applications

    Science.gov (United States)

    Rahman, Shekh; Carter, Princeton; Bhattarai, Narayan

    2017-01-01

    Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers. PMID:28216559

  9. Aloe Vera for Tissue Engineering Applications.

    Science.gov (United States)

    Rahman, Shekh; Carter, Princeton; Bhattarai, Narayan

    2017-02-14

    Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  10. Aloe Vera for Tissue Engineering Applications

    Directory of Open Access Journals (Sweden)

    Shekh Rahman

    2017-02-01

    Full Text Available Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  11. Effects of Aloe barbadensis Mill. extract (AVH200®) on human blood T cell activity in vitro.

    Science.gov (United States)

    Ahluwalia, Bani; Magnusson, Maria K; Isaksson, Stefan; Larsson, Fredrik; Öhman, Lena

    2016-02-17

    Aloe barbadensis Mill. (Aloe vera) is a widely used medicinal plant well reputed for its diverse therapeutic applications. It has been used for thousands of years in folk medicine to treat various conditions and the Aloe vera gel has been reported to possess anti-inflammatory as well as immunostimulatory and immunomodulatory properties. However, the mode of action is still unclear. The aim of this study was determine the effects of two well-defined A. barbadensis Mill. extracts AVH200® and AVE200 on human blood T cells in vitro. Peripheral blood mononuclear cells (PBMC) from healthy donors were stimulated polyclonally in the presence or absence of AVH200® and AVE200. The T cell phenotype was investigated by flow cytometry, cell proliferation was determined by CFSE dye and thymidine assay, respectively and cytokine secretion was determined by MSD® Multi-Spot Assay system and ELISA. The presence of AVH200® resulted in a reduced expression of CD25 among CD3(+) T cells and suppression of T cell proliferation in a dose dependent manner. Furthermore, AVH200® reduced the expression of CD28 on CD3(+) T cells. AVH200® also reduced the secretion of IL-2, IFN-γ and IL-17A in PBMC cultures. The AVH200® dose dependent reduction in T cell activation and proliferation recorded in the cell cultures was not due to apoptosis or cell death. Additionally, AVH200® was found to be more effective as compared to AVE200 in reducing T cell activation and proliferation. AVH200® has the potential to reduce the activation, proliferation and cytokine secretion of healthy human blood T cells. Our study suggests that AVH200® has a suppressive effect on human blood T cells in vitro. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  12. Utilization of plant bioactives as feed additives for poultry: The effect of Aloe vera gel and its extract on performance of broilers

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    A.P Sinurat

    2003-10-01

    Full Text Available Feed additives are commonly added in poultry feed as a growth promotant or to improve feed efficiency. The most common feed additive used is antibiotic at sub-therapheutic doses, although there is a controversy on its impact on human health. Previous results showed that Aloe vera gel could improve feed efficiency in broilers and an in vitro study showed that the extract have an antibacterial effect. Therefore, a further experiment was designed to study the response of broilers to Aloe vera gel or its extract as feed additives. Aloe vera was prepared in dry gel or chloroform-extract and included in the diet at levels of 0.25; 0.50 and 1.00 g/kg (equal to dry gel. Standard diets with or without antibiotic were also formulated as control and a commercial diet was included for comparison. The diets were fed to broilers from day old to 5 weeks. Each treatment has 9 replicates and 6chicks/replicate. Parameters observed were: feed consumption, weight gain and feed convertion ratios. Carcass yield, abdominal fat levels, relative weight of liver, gizzard, tractus digestivus and length of tractus digestivus were also measured at the end of feeding trial. The results showed that Aloe gel and its extract did not influence body weight gain and feed consumption of broilers significantly (P>0.05, but improved feed convertion slightly (3.50%. The response in this trial was similar as thosecommercial diet and diet added with antibiotic. There was no significant (P>0.05 effect of Aloe vera bioactives on carcass yield, abdominal fat level and relative weight of liver. However, Aloe vera gel and its extract tend to increase gizzard weight, gastro intestinal weight and length. The Aloe vera gel and its extract also reduced the total count of aerobic bacteria in the digesta of tractus digestivus. It is concluded that the Aloe vera gel improve feed efficiency in broilers by increasing the size of tractus digestivus and reducing the total count of aerobic bacteria in

  13. The effects of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid- induced gastric ulcer in male rats.

    Science.gov (United States)

    Keshavarzi, Zakieh; Rezapour, Taha Mohammad; Vatanchian, Mehran; Zare Hesari, Mohammad; Nabizade Haghighi, Hadi; Izanlu, Mostafa; Sabaghian, Maryam; Shahveisi, Kaveh

    2014-03-01

    Gut-brain axis (GBA) is very important in creation and modulation of gastrointestinal problems. Aloe vera gel has gastroprotective properties. The purpose of this study was to evaluate the effect of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid gastric ulcer induction. Gastric ulcer was induced by injection of 20% acetic acid into the subserosal layer in male rats. Rats were randomly assigned into three groups: intact group, gastric ulcer group and Aloe vera group (treatment with Aloe vera following gastric ulcer induction). The acid levels and brain and intestinal water content of each sample were measured eight days after the gastric ulcer induction. Gastric acid levels were significantly decreased in Aloe vera group when compared with gastric ulcer group (pAloe vera groups with intact group. After Aloe vera administration, the amount of brain water content had no difference with intact and gastric ulcer groups (pAloe vera group was significantly reduced compared with intact group (pAloe vera group. The administration of Aloe vera has an inhibitory effect on the gastric acid output.

  14. Atypical, polyarticular lipoma arborescens in a child

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    Semnic Robert

    2016-01-01

    Full Text Available Introduction. Lipoma arborescens is a rare, tumor-like lesion commonly involving synovial joints and less commonly bursae and synovial tendon sheaths. Case Outline. We report a case of a 12-year-old boy with symmetric involvement of the bicipitoradial bursae, synovial sheaths of extensor compartments of both hands and medial ankles. The diagnosis of polyarticular lipoma arborescens was proposed on magnetic resonance (MR imaging and this diagnosis was histologically proven after biopsy of the bursae and later by open surgery of the synovial sheath of the right ankle tendons. Literature search was performed and twelve cases with polyarticular involvement were analyzed. Lipoma arborescens commonly involves suprapatellar recess of the knee and very rarely other joints or bursae. Histological analysis revealed an accompanying non-necrotizing granulomatous synovial inflammation. Conclusion. Polyarticular lipoma arborescens is a rare entity and symmetrical involvement of the joints other than the knees is exceedingly rare. MR imaging plays a significant role in the diagnostic protocol, and the characteristic fatty signal on MR imaging is highly suggestive of lipoma arborescens.

  15. In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts

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    Gargouri Youssef

    2011-02-01

    Full Text Available Abstract Background In the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS extracts by using various solvents (hexane, chloroform-ethanol (1/1, ethyl acetate, butanol and water. We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by measuring their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA. Results The water extract exhibits the highest inhibitory effect with an IC50 = 0.22 mg/ml and interestingly no effect was observed on the digestive phospholipase A2 (group IB even at a concentration of 5 mg/ml. Antioxidant activities were also analyzed and the most active extracts were observed when using chloroform ethanol (1/1 and ethyl acetate (IC50 = 0.274 and 0.326 mg/ml, respectively. Analysis of the total phenolic content reveals that the water extract, with the best anti-PLA2 effect, was poor in phenolic molecules (2 mg GAE/g. This latter value has to be compared with the chloroform-ethanol and the ethyl acetate extracts (40 and 23.8 mg GAE/g, respectively, mostly responsible for the antioxidant activity. Conclusion A significant correlation was established between the total phenolic content and the antioxidant capacity but not with the anti PLA2 activity. Results from phytochemical screening suggest that the anti PLA2 molecules were probably catechin tannins compounds.

  16. Comparison of the antibacterial effect of sodium hypochlorite and aloe vera solutions as root canal irrigants in human extracted teeth contaminated with enterococcus faecalis.

    Science.gov (United States)

    Sahebi, S; Khosravifar, N; Sedighshamsi, M; Motamedifar, M

    2014-03-01

    The main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. Sodium hypochlorite has excellent antibacterial properties, but also some negative features. The aim of the present study is to compare the antimicrobial effect of Aloe Vera solution with sodium hypochlorite on E.faecalis in the root canals of human extracted teeth. Sixty human extracted single rooted teeth were selected for this in vitro study. The teeth recruited in this study had no cracks, internal resorption, external resorption and calcification. Enterococcus faecalis was injected in the root canals of all teeth. The teeth were then divided into three groups randomly. Each group consisted of 20 teeth that were all rinsed with one of the following solutions: sodium hypochlorite 2.5%, Aloe vera and normal saline. Subsequent to rinsing, root canals of all teeth were sampled. The samples were cultured and growth of the bacteria was assessed after 48 hours. The number of colonies of the bacteria was then counted. The difference between the inhibitory effect of Aloe vera and normal saline on E.faecalis was not significant according to independent t-test (p= 0.966). The inhibitory effect of sodium hypochlorite on E.faecalis was much greater than that of Aloe vera and normal saline (pAloe vera solution is not recommended as a root canal irrigator, but future studies are suggested to investigate the antibacterial effect of Aloe vera with longer duration of exposure and as an intra canal medicament.

  17. Humoral responses of broiler chickens challenged with NDV following supplemental treatment with extracts of Aloe vera, Alma millsoni, Ganoderma lucidum and Archachatina marginata.

    Science.gov (United States)

    Ojiezeh, Tony I; Eghafona, Nosahkare'Odeh

    2015-01-01

    The significance of nutritional supplements for immunity has been documented. Locally sourced extracts used in alternative medicine were studied to determine their potential effects on antibody production and humoral responses in viral challenged birds. Three hundred and eighty birds were distributed into 19 groups of 20 birds each. Following acclimatization for 16 days, they were fed with standard broilers feed and water ad libitum. Group A was supplemented with Aloe vera (AV) extract, group B was given Alma millsoni (AM) extract, group C was given Archachatina marginata (AMS) extract and group D was given Ganoderma lucidum (GL) extract, and group E was the control group. Extract concentrations of 50 mg, 100 mg and 150 mg were given to three subsets of each treatment group for 30 days. Birds were then challenged with intramuscular administration of 0.2 ml of 50% Embryo Lethal Dose of saline suspension of the challenge strain of Newcastle Disease Virus (NDV) on the 30(th) day, and were examined for clinical signs and symptoms. Serum from venous blood was used for antibody and immunological assay. Aloe vera at 50 µg and A. millsoni extracts supplementations yielded a significant antibody titre (p marginata, pretreatment with A. millsoni extract and a lower dosage of Aloe vera enhanced the ability to mount humoral responses against viral infection in broiler chickens.

  18. The use of 90% Aloe vera freeze drying as the modulator of collagen density in extraction socket of incicivus Cavia cobaya

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    Ester Arijani

    2008-06-01

    Full Text Available Wound healing is basically a complex process in which cellular and matrix act in concern to re-establish the integrity of injury tissues. This process can be simplified to be healing process consisted of haemostatic, inflammation, cell proliferation and tissue remodeling. The aimed of this research was to know the influence of freeze drying 90% Aloe vera application as mandible collagen density modulator in extraction socket of incisive Cavia cobaya. This research was done using Post Test Only Control Groups Design and Cavia cobaya as the sample. Six samples of each control group and 90% Aloe vera group applied to test each collagen density for three days and seven days. Then, the data was analyzed statistically using Mann Whitney with 5% significance rate. The result of the study indicates that administering 90% Aloe vera can accelerate the growth of collagen density in healing process of extraction socket. The conclusion is 90% Aloe vera can modulate the density of collageneous fiber in socket of extraction incicivus tooth wound of Cavia cobaya.

  19. Aloe vera plant-extracted solution hydrothermal synthesis and magnetic properties of magnetite (Fe3O4) nanoparticles

    Science.gov (United States)

    Phumying, Santi; Labuayai, Sarawuth; Thomas, Chunpen; Amornkitbamrung, Vittaya; Swatsitang, Ekaphan; Maensiri, Santi

    2013-06-01

    Magnetite (Fe3O4) nanoparticles have been successfully synthesized by a novel hydrothermal method using ferric acetylacetonate (Fe(C5H8O2)3) and aloe vera plant-extracted solution. The influences of different reaction temperatures and times on the structure and magnetic properties of the synthesized Fe3O4 nanoparticles were investigated. The synthesized nanoparticles are crystalline and have particle sizes of ˜6-30 nm, as revealed by transmission electron microscopy (TEM). The results of X-ray diffraction (XRD), High resolution TEM (HRTEM) and selected area electron diffraction (SAED) indicate that the synthesized Fe3O4 nanoparticles have the inverse cubic spinel structure without the presence of any other phase impurities. The hysteresis loops of the Fe3O4 nanoparticles at room temperature show superparamagnetic behavior and the saturation magnetization of the Fe3O4 samples increases with increasing reaction temperature and time.

  20. Aloe vera

    Energy Technology Data Exchange (ETDEWEB)

    Klein, A.D.; Penneys, N.S.

    1988-04-01

    We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials. 27 references.

  1. Comparison of the Antibacterial Effect of Sodium Hypochlorite and Aloe Vera Solutions as Root Canal Irrigants in Human Extracted Teeth Contaminated with Enterococcus Faecalis

    OpenAIRE

    Sahebi S.; Khosravifar N.; SedighShamsi M.; Motamedifar M.

    2014-01-01

    Statement of Problem: The main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. Sodium hypochlorite has excellent antibacterial properties but also some negative features. Purpose: The aim of the present study is to compare the antimicrobial effect of Aloe Vera solution with sodium hypochlorite on E.faecalis in the root canals of human extracted teeth. Materials and Method: Sixty human extracted single rooted teeth were selected for...

  2. Effect of acemannan, an extracted polysaccharide from Aloe vera, on BMSCs proliferation, differentiation, extracellular matrix synthesis, mineralization, and bone formation in a tooth extraction model.

    Science.gov (United States)

    Boonyagul, Sani; Banlunara, Wijit; Sangvanich, Polkit; Thunyakitpisal, Pasutha

    2014-07-01

    Aloe vera is a traditional wound healing medicine. We hypothesized acemannan, a polysaccharide extracted from Aloe vera gel, could affect bone formation. Primary rat bone marrow stromal cells (BMSCs) were treated with various concentrations of acemannan. New DNA synthesis, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein, osteopontin expression, and mineralization were determined by [(3)H] thymidine incorporation assay, ELISA, biochemical assay, western blotting, and Alizarin Red staining, respectively. In an animal study, mandibular right incisors of male Sprague-Dawley rats were extracted and an acemannan treated sponge was placed in the socket. After 1, 2, and 4 weeks, the mandibles were dissected. Bone formation was evaluated by dual-energy X-ray absorptiometry and histopathological examination. The in vitro results revealed acemannan significantly increased BMSC proliferation, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein and osteopontin expression, and mineralization. In-vivo results showed acemannan-treated groups had higher bone mineral density and faster bone healing compared with untreated controls. A substantial ingrowth of bone trabeculae was observed in acemannan-treated groups. These data suggest acemannan could function as a bioactive molecule inducing bone formation by stimulating BMSCs proliferation, differentiation into osteoblasts, and extracellular matrix synthesis. Acemannan could be a candidate natural biomaterial for bone regeneration.

  3. Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na{sup 99m}TcO{sub 4}) in rats

    Energy Technology Data Exchange (ETDEWEB)

    Holanda, Cecilia Maria de Carvalho Xavier [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. of Microbiology and Parasitology. Experimental Radiobiology and Antiparasitic Assays Lab.], e-mail: cechol@ufrnet.br; Costa, Monique Batista da; Silva, Natalia Chilinque Zambao da; Silva Junior, Mauricio Ferreira da; Barbosa, Vanessa Santos de Arruda; Silva, Roseane Pereira da [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil); Medeiros, Aldo da Cunha [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Nucleus of Experimental Surgery

    2009-07-01

    Purpose: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na{sup 99m}TcO{sub 4} and laboratorial parameters in Wistar rats. Methods: twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na{sup 99m}TcO{sub 4} by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (% ATI/g) was determined. Biochemical dosages were performed. Results: there was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p<0.05). Conclusion: The aqueous extract of Aloe vera facilitated the uptake of Na{sup 99m}TcO{sub 4} in organs of rats and it was responsible to a high increase of levels of AST and ALT. (author)

  4. Investigation on structural properties of M-type strontium hexaferrite synthesized in presence of neem and aloe-vera plant leaves extract

    Science.gov (United States)

    Solanki, Neha; Jotania, Rajshree B.

    2017-05-01

    M-type strontium hexaferrite powder samples were synthesized using a green synthesis route with and without presence of Aloe vera and Neem leaves extract. The dry brownish precursors of strontium hexaferrite were recovered from a mixed solution of metal salts and leaves extract, heated at 100 °C. The obtained precursors were pre-heated at 500 °C for 4 hrs. followed by final heating at 950 °C for 4 hrs. in a muffle furnace to obtain SrFe12O19 hexaferrite powder. The obtained SrFe12O19 hexaferrite powder samples characterized at room temperature in order to check phase purity and structural properties. XRD analysis confirms that samples prepared without and with Aloe vera leaves extract (heated at 950 °C for 4 hrs.) show formation of α-Fe2O3 and M-phase; while the sample prepared in presence of Neem leaves extract (heated at 950 °C for 4 hrs.) show formation of mono phase of strontium hexaferrite. Lattice parameter (a) and cell volume (V) are found to increase in the samples prepared in presence of Aloe vera and Neem leaves extract.

  5. The effects of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid- induced gastric ulcer in male rats

    Directory of Open Access Journals (Sweden)

    Zakieh Keshavarzi

    2014-02-01

    Full Text Available Objective: Gut–brain axis (GBA is very important in creation and modulation of gastrointestinal problems. Aloe vera gel has gastroprotective properties. The purpose of this study was to evaluate the effect of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid gastric ulcer induction. Materials and Methods: Gastric ulcer was induced by injection of 20% acetic acid into the subserosal layer in male rats. Rats were randomly assigned into three groups: intact group, gastric ulcer group and Aloe vera group (treatment with Aloe vera following gastric ulcer induction. The acid levels and brain and intestinal water content of each sample were measured eight days after the gastric ulcer induction. Results: Gastric acid levels were significantly decreased in Aloe vera group when compared with gastric ulcer group (p

  6. [Identification of Aloe species by random amplified polymorphic DNA (RAPD) analysis].

    Science.gov (United States)

    Shioda, Hiroko; Satoh, Kanako; Nagai, Fumiko; Okubo, Tomoko; Seto, Takako; Hamano, Tomoko; Kamimura, Hisashi; Kano, Itsu

    2003-08-01

    Juice and integument of leaves of 3 Aloe species, Aloe vera, A. ferox and A. africana, are not allowed to be used as food according to the Pharmaceutical Affairs Law in Japan. On the other hand, whole leaves of A. arborescens can be used as food. The present study was designed to distinguish Aloe species by random amplified polymorphic DNA (RAPD) analysis. DNA was isolated from fresh and dried leaves of the 4 Aloe species. Five out of 32 different 10-mer primers examined were useful for analysis. By comparison of the characteristic bands of PCR products on agarose gel, it was possible to distinguish the 4 species. Thus, the botanical species of Aloe in commercial food products can be identified by RAPD analysis.

  7. Chemical Composition of the Essential Oil from Artemisia arborescens L. Growing Wild in Algeria

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    Gilles Figuérédo

    2010-01-01

    Full Text Available Essential oil extracted from dried aerial part of Artemisia arborescens L. collected from Bejaïa (Algeria, was analyzed by gas chromatography-flame ionization detector (GC-FID and gas chromatography coupled to mass spectrometry (GC-MS. The main constituents of the essential oil were chamazulene (30.2%, β-thujone (27.8%, β-eudesmol (8.1% and catalponol (5.5%.

  8. Improvement of Insulin Secretion and Pancreatic β-cell Function in Streptozotocin-induced Diabetic Rats Treated with Aloe vera Extract.

    Science.gov (United States)

    Noor, Ayesha; Gunasekaran, S; Vijayalakshmi, M A

    2017-12-01

    Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. Plant extracts and their products are being used as an alternative system of medicine for the treatment of diabetes. Aloe vera has been traditionally used to treat several diseases and it exhibits antioxidant, anti-inflammatory, and wound-healing effects. Streptozotocin (STZ)-induced Wistar diabetic rats were used in this study to understand the potential protective effect of A. vera extract on the pancreatic islets. The aim of the present study was to evaluate the A. vera extract on improvement of insulin secretion and pancreatic β-cell function by morphometric analysis of pancreatic islets in STZ-induced diabetic Wistar rats. After acclimatization, male Wistar rats, maintained as per the Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines, were randomly divided into four groups of six rats each. Fasting plasma glucose and insulin levels were assessed. The effect of A. vera extract in STZ-induced diabetic rats on the pancreatic islets by morphometric analysis was evaluated. Oral administration of A. vera extract (300 mg/kg) daily to diabetic rats for 3 weeks showed restoration of blood glucose levels to normal levels with a concomitant increase in insulin levels upon feeding with A. vera extract in STZ-induced diabetic rats. Morphometric analysis of pancreatic sections revealed quantitative and qualitative gain in terms of number, diameter, volume, and area of the pancreatic islets of diabetic rats treated with A. vera extract when compared to the untreated diabetic rats. A. vera extract exerts antidiabetic effects by improving insulin secretion and pancreatic β-cell function by restoring pancreatic islet mass in STZ-induced diabetic Wistar rats. Fasting plasma glucose (FPG) and insulin levels were restored to normal levels in diabetic rats treated with Aloe vera extractIslets of pancreas were qualitatively and quantitatively restored to

  9. The influence of Aloe vera and xenograft XCB toward of bone morpho protein 2 BMP2 expression and amount of osteoblast of alveolar bone induced into tooth extraction sockets Cavia cobaya

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2014-12-01

    Full Text Available Tooth extraction can cause inflammation leading to alveolar ridge resorption. In addition, prominent ridge has crucial role for making denture su-ccessfully. Thus, socket preservation is needed to prevent greater alveolar ridge resorption. An innovative material, a combination of Aloe vera and xe-nograft (XCB, is then considered as a biogenic stimulator that can reduce inflammation, as a result, the growth of alveolar bone is expected to be impro-ved. This research is aimed to prove whether the mixture of Aloe vera and xenograft can stimulate BMP2 and increase osteoblasts. Forty-eight Cavia co-baya animals were divided into eight groups each of which consisted of six animals. The mandibular incisors of those Cavia cobaya animals were then extracted and filled with PEG as Group Control, XCB as Group XCB, Aloe vera as Group Aloe vera, and a combination of Aloe vera +XCB as Group Aloe vera +XCB. Next, the first four groups were sacrificed seven days after extraction, and the second four groups were sacrificed 30 days after extrac-tion. And then, immunohistochemical and histopathology examinations were conducted to examine BMP2 expression and osteoblasts. Based on the re-sult known that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblasts. It can be concluded that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblast cel . It can be used as an alternative material to increase the growth of alveolar bone after extraction.

  10. Automated Control of the Organic and Inorganic Composition of Aloe vera Extracts Using (1)H NMR Spectroscopy.

    Science.gov (United States)

    Monakhova, Yulia B; Randel, Gabriele; Diehl, Bernd W K

    2016-09-01

    Recent classification of Aloe vera whole-leaf extract by the International Agency for Research and Cancer as a possible carcinogen to humans as well as the continuous adulteration of A. vera's authentic material have generated renewed interest in controlling A. vera. The existing NMR spectroscopic method for the analysis of A. vera, which is based on a routine developed at Spectral Service, was extended. Apart from aloverose, glucose, malic acid, lactic acid, citric acid, whole-leaf material (WLM), acetic acid, fumaric acid, sodium benzoate, and potassium sorbate, the quantification of Mg(2+), Ca(2+), and fructose is possible with the addition of a Cs-EDTA solution to sample. The proposed methodology was automated, which includes phasing, baseline-correction, deconvolution (based on the Lorentzian function), integration, quantification, and reporting. The NMR method was applied to 41 A. vera preparations in the form of liquid A. vera juice and solid A. vera powder. The advantages of the new NMR methodology over the previous method were discussed. Correlation between the new and standard NMR methodologies was significant for aloverose, glucose, malic acid, lactic acid, citric acid, and WLM (P < 0.0001, R(2) = 0.99). NMR was found to be suitable for the automated simultaneous quantitative determination of 13 parameters in A. vera.

  11. Immune responses induced in rabbits after oral administration of bovine serum albumin in combination with different adjuvants (herb extracts, aluminium hydroxide and platinum nanoparticles

    Directory of Open Access Journals (Sweden)

    G. Bižanov

    2016-12-01

    Full Text Available The aim of the current study was to evaluate the immunostimulatory activity of 10 different herbal extracts from Vitex agnus-castus, Vinca major, Aloe arborescens and the polyherbal product containing extracts from Sambucus nigra, Primula versis, Pinus alba, Gentiana lutea, Cetraria islandica, Eucaliptus globulus, Citrus limon and aluminium hydroxide, as well as platinum nanoparticles. Rabbits were immunized three times orally with bovine serum albumin (BSA in combination with the components mentioned above. BSA-specific IgA antibodies in saliva and IgG antibodies in serum were examined by ELISA. It was found that the rabbits immunized with BSA in combination with either platinum nanoparticles or aluminium hydroxide had higher titres of BSA-specific IgA antibodies in their saliva at day 56 of observation. Likewise, rabbits treated with BSA and Vinca major or Aloe arborescens extracts showed higher levels of BSA-specific IgG antibodies in the serum at the end of observation. These results suggest that some plant extracts, aluminium hydroxide and platinum nanoparticles components could be used as oral adjuvants or as immunomodulators for rabbits.

  12. Mosquito larvicidal activity of Aloe vera (Family: Liliaceae) leaf extract and Bacillus sphaericus, against Chikungunya vector, Aedes aegypti.

    Science.gov (United States)

    Subramaniam, Jayapal; Kovendan, Kalimuthu; Mahesh Kumar, Palanisamy; Murugan, Kadarkarai; Walton, William

    2012-10-01

    The bio-efficacy of Aloe vera leaf extract and bacterial insecticide, Bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of Aedes aegypti, under the laboratory conditions. The plant material was shade dried at room temperature and powdered coarsely. A. vera and B. sphaericus show varied degrees of larvicidal activity against various instars larvae of A. aegypti. The LC50 of A. vera against the first to fourth instars larvae were 162.74, 201.43, 253.30 and 300.05 ppm and the LC90 442.98, 518.86, 563.18 and 612.96 ppm, respectively. B. sphaericus against the first to fourth instars larvae the LC50 values were 68.21, 79.13, 93.48, and 107.05 ppm and the LC90 values 149.15, 164.67, 183.84, and 201.09 ppm, respectively. However, the combined treatment of A. vera + B. sphaericus (1:2) material shows highest larvicidal activity of the LC50 values 54.80, 63.11, 74.66 and 95.10 ppm; The LC90 values of 145.29, 160.14, 179.74 and 209.98 ppm, against A. aegypti in all the tested concentrations than the individuals and clearly established that there is a substantial amount of synergist act. The present investigation clearly exhibits that both A. vera and B. sphaericus materials could serve as a potential larvicidal agent. Since, A. aegypti is a container breeder vector mosquito this user and eco-friendly and low-cost vector control strategy could be a viable solution to the existing dengue disease burden. Therefore, this study provides first report on the mosquito larvicidal activity the combined effect of A. vera leaf extract and B. sphaericus against as target species of A. aegypti.

  13. Aloe Vera

    Science.gov (United States)

    ... month drinking water toxicity study in F344 rats . Food and Chemical Toxicology . 2013;57:21-31. Yang HN, Kim DJ, Kim YM, et al. Aloe-induced toxic hepatitis . Journal of Korean Medical Science. 2010;25(3):492-495. This publication is ...

  14. Anti sickling potential of Aloe vera extract IV: Effects of acidic, basic ...

    African Journals Online (AJOL)

    The effects of acidic, basic and neutral metabolites from A. vera extract on the sickling and gelling time of human HbSS erythrocytes were investigated. The study showed that the various metabolites inhibited sickling in vitro and increased gelling time of HbSS blood samples. Moreover, a significant increase in the number of ...

  15. Water-soluble egg membrane enhances the immunoactivating properties of an Aloe vera-based extract of Nerium oleander leaves

    Directory of Open Access Journals (Sweden)

    Benson KF

    2016-11-01

    Full Text Available Kathleen F Benson,1 Robert A Newman,2,3 Gitte S Jensen1 1NIS Labs, Klamath Falls, OR, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center, Houston, 3Nerium Biotechnology Inc, San Antonio, TX, USA Objective: To evaluate a blend of two natural ingredients on immune parameters relevant for their current topical use and potential support of microcirculation in skin tissue. Materials and methods: A blend (BL of Aloe vera-based Nerium oleander extract (NAE-8i, oleandrin-free and hydrolyzed water-soluble egg membrane (WSEM was applied to human whole-blood cultures for 24 hours, with each separate ingredient serving as a control. Immune-cell subsets were analyzed for expression levels of the activation markers CD69 and CD25. Culture supernatants were analyzed for cytokines, chemokines, and immunoregulating peptides. Results: BL increased CD69 expression on lymphocytes, monocytes, and CD3–CD56+ natural killer cells, and CD25 expression on natural killer cells. The number of CD69+CD25+ lymphocytes increased in cultures treated with BL and the separate ingredients. BL triggered production of multiple cytokines and chemokines, where CC chemokines MIP1α and MIP3α, as well as cytokines involved in wound healing – Groα, Groβ, ENA78, and fractalkine – reached levels manyfold above treatment with either NAE-8i or WSEM alone. Conclusion: Data on BL showed that WSEM strongly enhanced NAE-8i’s effects on immunoactivation in vitro. This has potential relevance for support of immunity in skin tissue, including antibacterial and antiviral defense mechanisms, wrinkle reduction, and wound care. Keywords: chemokines, cytokines, leukocyte activation

  16. Allergic contact dermatitis to Aloe vera

    OpenAIRE

    Ferreira, M.; Teixeira, M.; Silva, E.; Selores, M

    2007-01-01

    Abstract We present the case of a 72-year-old woman observed for dermatitis on the legs followed by apperance of erythema on the eyelids. She had a past history of peripheral venous insufficiency and had been using self home-made Aloe vera juice over the legs for relief from pain. Patch tests showed positive reactions to the leaf of Aloe, the macerated Aloe jelly, and nickel sulfate. Although most manufacturers process Aloe products avoiding its irritant extracts, and probably as a consequ...

  17. Artemisia arborescens Essential Oil Composition, Enantiomeric Distribution, and Antimicrobial Activity from Different Wild Populations from the Mediterranean Area.

    Science.gov (United States)

    Said, Mohammed El-Amin; Militello, Marcello; Saia, Sergio; Settanni, Luca; Aleo, Aurora; Mammina, Caterina; Bombarda, Isabelle; Vanloot, Pierre; Roussel, Christian; Dupuy, Nathalie

    2016-08-01

    Aerial parts of Artemisia arborescens were collected from different sites of the Mediterranean area (southwestern Algeria and southern Italy) and the chemical composition of their essential oil (EO) extracted by hydrodistillation was studied by both gas chromatography (GC) equipped with an enantioselective capillary column and GC/mass spectrometry (GC/MS). The EOs obtained were tested against several Listeria monocytogenes strains. Using GC and GC/MS, 41 compounds were identified, accounting for 96.0 - 98.8% of the total EO. All EOs showed a similar terpene profile, which was rich in chamazulene, β-thujone, and camphor. However, the concentration of such compounds varied among the EOs. A. arborescens EO inhibited up to 83.3% of the L. monocytogenes strains, but the inhibitory spectrum varied among the EOs, with those from Algeria showing a higher inhibition degree than the Italian EOs. Such effect likely depended on the ketone (β-thujone + camphor) content of the EO. The differences in the EO composition support the hypothesis that A. arborescens has at least two different chemotypes: a β-thujone and a chamazulene type. The EO inhibitory spectrum indicates the A. arborescens EO as a valuable option in the control of the food-borne pathogens. © 2016 Wiley-VHCA AG, Zürich.

  18. Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)?A Review

    OpenAIRE

    Barbara De Lucia; Bernardo Murillo-Amador; Giuseppe Cristiano

    2016-01-01

    Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important fac...

  19. Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review

    Science.gov (United States)

    Radha, Maharjan H.; Laxmipriya, Nampoothiri P.

    2014-01-01

    Aloe vera (蘆薈 lú huì) is well known for its considerable medicinal properties. This plant is one of the richest natural sources of health for human beings coming. The chemistry of the plant has revealed the presence of more than 200 different biologically active substances. Many biological properties associated with Aloe species are contributed by inner gel of the leaves. Most research has been centralized on the biological activities of the various species of Aloe, which include antibacterial and antimicrobial activities of the nonvolatile constituents of the leaf gel. Aloe species are widely distributed in the African and the eastern European continents, and are spread almost throughout the world. The genus Aloe has more than 400 species but few, such as A. vera, Aloe ferox, and Aloe arborescens, are globally used for trade. A. vera has various medicinal properties such as antitumor, antiarthritic, antirheumatoid, anticancer, and antidiabetic properties. In addition, A. vera has also been promoted for constipation, gastrointestinal disorders, and for immune system deficiencies. However, not much convincing information is available on properties of the gel. The present review focuses on the detailed composition of Aloe gel, its various phytocomponents having various biological properties that help to improve health and prevent disease conditions. PMID:26151005

  20. Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review

    Directory of Open Access Journals (Sweden)

    Maharjan H. Radha

    2015-01-01

    Full Text Available Aloe vera (蘆薈 lú huì is well known for its considerable medicinal properties. This plant is one of the richest natural sources of health for human beings coming. The chemistry of the plant has revealed the presence of more than 200 different biologically active substances. Many biological properties associated with Aloe species are contributed by inner gel of the leaves. Most research has been centralized on the biological activities of the various species of Aloe, which include antibacterial and antimicrobial activities of the nonvolatile constituents of the leaf gel. Aloe species are widely distributed in the African and the eastern European continents, and are spread almost throughout the world. The genus Aloe has more than 400 species but few, such as A. vera, Aloe ferox, and Aloe arborescens, are globally used for trade. A. vera has various medicinal properties such as antitumor, antiarthritic, antirheumatoid, anticancer, and antidiabetic properties. In addition, A. vera has also been promoted for constipation, gastrointestinal disorders, and for immune system deficiencies. However, not much convincing information is available on properties of the gel. The present review focuses on the detailed composition of Aloe gel, its various phytocomponents having various biological properties that help to improve health and prevent disease conditions.

  1. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (NAE-8®

    Directory of Open Access Journals (Sweden)

    Benson KF

    2015-05-01

    Full Text Available Kathleen F Benson,1 Robert A Newman,2,3 Gitte S Jensen1 1NIS Labs, Klamath Falls, Oregon, USA; 2University of Texas MD Anderson Cancer Center, Houston, TX, USA; 3Nerium Biotechnology, Inc, San Antonio, TX, USA Objective: The goal for this study was to evaluate the effects of an Aloe vera-based Nerium oleander extract (NAE-8®, compared to an extract of A. vera gel alone (ALOE, and to an aqueous extract of N. oleander (AQ-NOE in bioassays pertaining to dermatologic potential with respect to antioxidant protection, anti-inflammatory effects, and cytokine profiles in vitro. Methods: Cellular antioxidant protection was evaluated in three separate bioassays: The cellular antioxidant protection of erythrocytes (CAP-e assay, protection of cellular viability and prevention of apoptosis, and protection of intracellular reduced glutathione levels, where the last two assays were performed using human primary dermal fibroblasts. Reduction of intracellular formation of reactive oxygen species (ROS was tested using polymorphonuclear cells in the absence and presence of oxidative stress. Changes to cytokine and chemokine profiles when whole blood cells and human primary dermal fibroblasts were exposed to test products were determined using a 40-plex Luminex array as a method for exploring the potential cross-talk between circulating and skin-resident cells. Results: The NAE-8® provided significantly better antioxidant protection in the CAP-e bioassay than AQ-NOE. NAE-8® and AQ-NOE both protected cellular viability and intracellular reduced glutathione, and reduced the ROS formation significantly when compared to control cells, both under inflamed and neutral culture conditions. ALOE showed minimal effect in these bioassays. In contrast to the NAE-8®, the AQ-NOE showed induction of inflammation in the whole blood cultures, as evidenced by the high induction of CD69 expression and secretion of a number of inflammatory cytokines. The treatment of dermal

  2. Reduction of intestinal polyp formation in min mice fed a high-fat diet with aloe vera gel extract.

    Science.gov (United States)

    Chihara, Takeshi; Shimpo, Kan; Beppu, Hidehiko; Tomatsu, Akiko; Kaneko, Takaaki; Tanaka, Miyuki; Yamada, Muneo; Abe, Fumiaki; Sonoda, Shigeru

    2013-01-01

    Aloe vera gel supercritical CO2 extract (AVGE) has been shown to contain five phytosterols, reduce visceral fat accumulation, and influence the metabolism of glucose and lipids in animal model experiments. Recent epidemiologic studies have shown that obesity is an established risk factor for several cancers including colorectal cancer. Therefore, we examined the effects of AVGE on intestinal polyp formation in Apc-deficient Min mice fed a high-fat diet. Male Min mice were divided into normal diet (ND), high fat diet (HFD), low dose AVGE (HFD+LAVGE) and high dose AVGE (HFD+HAVGE) groups. The ND group received AIN-93G diet and the latter 3 groups were given modified high-fat AIN-93G diet (HFD) for 7 weeks. AVGE was suspended in 0.5% carboxymethyl cellulose (CMC) and administered orally to mice in HFD+LAVGE and HFD+HAVGE groups every day (except on Sunday) for 7 weeks at a dose of 3.75 and 12.5 mg/kg body weight, respectively. ND and HFD groups received 0.5% CMC alone. Between weeks 4 and 7, body weights in the HFD and HFD+LAVGE groups were reduced more than those in the ND group. However, body weights were not reduced in the HFD+HAVGE group. Mice were sacrificed at the end of the experiment and their intestines were scored for polyps. No significant differences were observed in either the incidence and multiplicity of intestinal polyps (≥0.5 mm in a diameter) among the three groups fed HFD. However, when intestinal polyps were categorized by their size into 0.5-1.4, 1.5-2.4, or ≥2.5 mm, the incidence and multiplicity of large polyps (≥2.5 mm) in the intestine in the HFD+HAVGE group were significantly lower than those in the HFD group. We measured plasma lipid (triglycerides and total cholesterol) and adipocytokine [interleukin-6 and high molecular weight (HMW) adiponectin] levels as possible indicators of mechanisms of inhibition. The results showed that HMW adiponectin levels in the HFD group were significantly lower than those in the ND group. However, the

  3. Synovial lipomatosis arborescens of the peroneal tendon sheath

    Energy Technology Data Exchange (ETDEWEB)

    Vinson, Emily N.; Martinez, Salutario [Duke University Medical Center, Department of Radiology, Durham, NC (United States); Dodd, Leslie G. [Duke University Medical Center, Department of Pathology, Durham, NC (United States); Merian, Marc [Duke University Medical Center, Division of Orthopaedic Surgery, Durham, NC (United States)

    2008-10-15

    The term ''lipoma arborescens'' has been used to describe the diffuse infiltration of fat within hypertrophic synovial villi, a condition which has been most frequently described in the knee. We advocate the term ''synovial lipomatosis arborescens'' for this process and present what is, to our knowledge, the first reported case of this disorder isolated to the peroneal tendon sheath, with imaging, intraoperative, and histological correlation. (orig.)

  4. Comparative Evaluation of Fresh Aloe barbadensis Plant Extract and Mineral Trioxide Aggregate as Pulpotomy Agents in Primary Molars: A 12-month Follow-up Study

    Science.gov (United States)

    Kalra, Mehak; Garg, Nishita; Rallan, Mandeep; Pathivada, Lumbini; Yeluri, Ramakrishna

    2017-01-01

    Background: The purpose of this study was to compare the clinical and radiographic outcomes of fresh Aloe vera barbadensis plant extract and mineral trioxide aggregate (MTA) as pulpotomy agents in primary molar teeth. Materials and Methods: Pulpotomy procedure was performed in sixty primary molar teeth which were randomly allocated to two groups, i.e., Aloe vera pulpotomy (Group A) and MTA pulpotomy (Group B). All the pulpotomized teeth were evaluated clinically and radiographically at 1, 3, 6, 9, and 12 months of time interval using predetermined criteria. Results: The success rates between Groups A and B at the end of the 1st month were 24.1% and 96.4%, at the end of 3rd month were 57.1% and 100%, at the end of 6th month were 75% and 100%, at the end of 9th month were 66.6% and 100%, and at the end of 12 months were 100% and 100% respectively. The overall success rates at the end of 12-month follow-up period were 6.9% and 71.4%, respectively, after taking dropout patients into consideration, and the difference was statistically significant (P MTA pulpotomy was found to be superior when compared to fresh A. barbadensis plant extract pulpotomy in primary molars. PMID:28566860

  5. Comparative evaluation of fresh Aloe barbadensis plant extract and mineral trioxide aggregate as pulpotomy agents in primary molars: A 12-month follow-up study

    Directory of Open Access Journals (Sweden)

    Mehak Kalra

    2017-01-01

    Full Text Available Background: The purpose of this study was to compare the clinical and radiographic outcomes of fresh Aloe vera barbadensis plant extract and mineral trioxide aggregate (MTA as pulpotomy agents in primary molar teeth. Materials and Methods: Pulpotomy procedure was performed in sixty primary molar teeth which were randomly allocated to two groups, i.e., Aloe vera pulpotomy (Group A and MTA pulpotomy (Group B. All the pulpotomized teeth were evaluated clinically and radiographically at 1, 3, 6, 9, and 12 months of time interval using predetermined criteria. Results: The success rates between Groups A and B at the end of the 1st month were 24.1% and 96.4%, at the end of 3rd month were 57.1% and 100%, at the end of 6th month were 75% and 100%, at the end of 9th month were 66.6% and 100%, and at the end of 12 months were 100% and 100% respectively. The overall success rates at the end of 12-month follow-up period were 6.9% and 71.4%, respectively, after taking dropout patients into consideration, and the difference was statistically significant (P < 0.001. Conclusions: MTA pulpotomy was found to be superior when compared to fresh A. barbadensis plant extract pulpotomy in primary molars.

  6. Aloe vera on Lanzarote

    OpenAIRE

    Walker, Colin

    2016-01-01

    Aloe vera is the basis of a multibillion dollar cosmetics and toiletries industry worldwide. It is grown commercially in many parts of the world and here its cultivation is described on an aloe farm in the north of Lanzarote, the Canary Islands. The products from such monoculture are sold throughout the island in Aloe vera shops run principally by the company aloe Lanzarote.

  7. Wound Healing Activity of Extracts and Formulations of Aloe vera, Henna, Adiantum capillus-veneris, and Myrrh on Mouse Dermal Fibroblast Cells

    Science.gov (United States)

    Negahdari, Samira; Galehdari, Hamid; Kesmati, Mahnaz; Rezaie, Anahita; Shariati, Gholamreza

    2017-01-01

    Background: Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such as Adiantum capillus-veneris, Commiphora molmol, Aloe vera, and henna and one mixture of them were used to treatment of normal mouse skin fibroblasts. Methods: Cytotoxic effects of each extract and their mixture were assessed on mouse skin fibroblasts cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We performed migration assays to assess migration properties of mouse skin fibroblasts cells in response to the extracts. Changes in the gene expression of the Tgfβ1 and Vegf-A genes were monitored by real-time polymerase chain reaction. Results: A. capillus-veneris, C. molmol and henna extract improved the expression of Tgfβ1 gene. All used extracts upregulated the expression of Vegf-A gene and promoted the migration of mouse fibroblast cells in vitro. Conclusions: The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgfβ1 and Vegf-A genes in fibroblast cells treated with extracts. PMID:28382194

  8. Allergic contact dermatitis to Aloe vera.

    Science.gov (United States)

    Ferreira, Márcia; Teixeira, Marta; Silva, Elvira; Selores, Manuela

    2007-10-01

    We present the case of a 72-year-old woman observed for dermatitis on the legs followed by apperance of erythema on the eyelids. She had a past history of peripheral venous insufficiency and had been using self home-made Aloe vera juice over the legs for relief from pain. Patch tests showed positive reactions to the leaf of Aloe, the macerated Aloe jelly, and nickel sulfate. Although most manufacturers process Aloe products avoiding its irritant extracts, and probably as a consequence reports of allergic reactions are rare, one must remember that the growing popularity on the use of Aloe products may stimulate its use 'as is' by the patients. Furthermore, it is important to specifically ask patients about the use of these products, because they consider it as innocuous and thus would not spontaneously provide such information.

  9. Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

    Science.gov (United States)

    Cristiano, Giuseppe; Murillo-Amador, Bernardo; De Lucia, Barbara

    2016-01-01

    Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems. PMID:27721816

  10. PROPAGATION TECHNIQUES AND AGRONOMIC REQUIREMENTS FOR THE CULTIVATION OF BARBADOS ALOE (ALOE VERA (L. BURM. F. - A REVIEW.

    Directory of Open Access Journals (Sweden)

    Barbara De Lucia

    2016-09-01

    Full Text Available Barbados aloe (Aloe vera (L. Burm. f. has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe and America.In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

  11. In Vitro Antioxidant Effects of Aloe barbadensis Miller Extracts and the Potential Role of These Extracts as Antidiabetic and Antilipidemic Agents on Streptozotocin-Induced Type 2 Diabetic Model Rats

    Directory of Open Access Journals (Sweden)

    Md. Ibrahim Khalil

    2012-11-01

    Full Text Available In this study, the total phenolic and flavonoid contents, the 2,2-diphenyl-1-picryl hydrazyl (DPPH radical scavenging ability and the ferric reducing power (FRAP of Aloe vera were measured to determine the antioxidant activity of this species. The in vivo antidiabetic effects of the plant were also investigated using streptozotocin-induced type 2 diabetic model rats that were divided into five groups based on the treatment received: (1 water (WC; (2 glibenclamide; (3 concentrated gel extract (Gel-C; (4 ethanol (80% gel extract (Gel-Et; and (5 ethanol (80% skin extract of Aloe vera (Skin-Et. Skin-Et, which contained the highest level of total phenolics (62.37 ± 1.34 mggallic acid/kg and flavonoids (20.83 ± 0.77 mg/kg, exhibited the highest scavenging activity (85.01 ± 0.52% and the greatest reducing power (185.98 ± 0.41 µM, indicating that the skin contained the highest level of antioxidants. The oral consumption of Gel-Et for 4 weeks a caused significant reduction in the fasting serum glucose levels of the rats. The rats in the Gel-C-, Gel-Et- and Skin-Et-treated groups experienced a reduction in their total cholesterol levels by 11%, 17% and 25%, respectively and a reduction in their LDL cholesterol levels by 45%, 3% and 69%, respectively. The in vivo experimental antioxidant parameter MDA is strongly correlated with the in vitro antioxidant parameters of flavonoids and polyphenols, namely the DPPH and FRAP values (r = 0.94, 0.92, 0.93, 0.90, thus confirming the antioxidant potential of the Aloe vera extracts.

  12. Antispasmodic and antioxidant activities of fractions and bioactive constituent davidigenin isolated from Mascarenhasia arborescens.

    Science.gov (United States)

    Desire, Odile; Rivière, Céline; Razafindrazaka, René; Goossens, Laurence; Moreau, Stéphane; Guillon, Jean; Uverg-Ratsimamanga, Suzanne; Andriamadio, Pascal; Moore, Nicholas; Randriantsoa, Adolphe; Raharisololalao, Amélie

    2010-07-20

    Mascarenhasia arborescens A. DC. (Apocynaceae) is used in traditional medicine in the North of Madagascar to treat intestinal disorders, intestinal spasms and diarrhoea. The main objective of this work was to evaluate the antispasmodic activity of the crude methanolic extract of Mascarenhasia arborescens and of its four partitions and to identify the effective compound responsible for this effect. Isolation and structure elucidation techniques were performed in order to identify the bioactive constituent of Mascarenhasia arborescens and HPLC analysis was used for its quantification. Total phenolic content (TPC) of crude extracts and partitions were determined using the Folin-Ciocalteu method. Crude methanolic extract, partitions and the bioactive compound were investigated for their spasmolytic activity on several isolated organs. Their antiradical activity was also investigated by the DPPH test. Bioassay-guided fractionation using isolated guinea pig ileum pre-contracted with histamine 3x10(-6) M led to the isolation of davidigenin (DG), a dihydrochalcone, as the main active constituent from the most promising methylene chloride partition (McP). This partition was effective on isolated guinea pig ileum pre-contracted with 3x10(-6) M histamine, with a median effective concentration (EC(50)) of 41.19+/-3.74 microg/ml. The DG content of this partition was shown to be 26.5% by HPLC. DG induced a concentration-dependent relaxation of the histamine pre-contracted guinea pig ileum with an EC(50) of 8.04+/-0.81 microg/ml and a concentration-dependent relaxation of the acetylcholine pre-contracted rat duodenum with an EC(50) of 9.35+/-0.30 microg/ml. It inhibited in a non-competitive manner histamine-induced isolated ileum contraction and the acetylcholine-induced isolated duodenum contraction. Moreover, DG does not have any antiradical activity. We demonstrated for the first time antispasmodic and antioxidant effects of Mascarenhasia arborescens. This study supports its

  13. Phytochemical screening of Artemisia arborescens L. by means of advanced chromatographic techniques for identification of health-promoting compounds.

    Science.gov (United States)

    Costa, Rosaria; Ragusa, Salvatore; Russo, Marina; Certo, Giovanna; Franchina, Flavio A; Zanotto, Antonio; Grasso, Elisa; Mondello, Luigi; Germanò, Maria Paola

    2016-01-05

    Artemisia arborescens, also known as tree wormwood, is a typical species of the Mediterranean flora. It has been used in folk medicine for its antispasmodic, anti-pyretic, anti-inflammatory, and abortifacient properties. In the current study, the application of multidimensional comprehensive gas chromatography (GC×GC), allowed to obtain a detailed fingerprint of the essential oil from A. arborescens aerial parts, highlighting an abundant presence of chamazulene followed by camphor, β-thujone, myrcene, and α-pinene. Moreover, flavonoids in the dichloromethane extract were analyzed by means of liquid chromatography with photodiode array and atmospheric pressure chemical ionization-mass spectrometry detections (HPLC-PDA and HPLC-APCI-MS). Six polymethoxyflavones were identified and three of them, including chrysosplenetin, eupatin, and cirsilineol, were described in this species for the first time. The anti-angiogenic activity was investigated in the dichloromethane extract by two in vivo models, chick chorioallantoic membrane (CAM) and zebrafish embryos. Results showed that this extract produced a strong reduction on vessel formation, both on zebrafish (57% of inhibition, 0.1 mg/mL) and chick chorioallantoic membrane (58% of inhibition, 0.8 mg/mL). The high separation power and sensitivity of the analytical methodology applied confirmed the safety of A. arborescens essential oil for human consumption, due to the very low level of the psychotrope α-thujone determined. Moreover, the knowledge of the flavonoidic profile holds a great significance for the use of A. arborescens as a valuable source of anti-angiogenic compounds that might contribute to the valorization of the phytotherapeutic potential of this plant. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. Feeding mice with Aloe vera gel diminishes L-1 sarcoma-induced early neovascular response and tumor growth.

    Science.gov (United States)

    Kocik, Janusz; Bałan, Barbara Joanna; Zdanowski, Robert; Jung, Leszek; Skopińska-Różewska, Ewa; Skopiński, Piotr

    2014-01-01

    Aloe vera (Aloe arborescens, aloe barbadensis) is a medicinal plant belonging to the Liliaceae family. Aloe vera gel prepared from the inner part of Aloe leaves is increasingly consumed as a beverage dietary supplement. Some data suggest its tumor growth modulatory properties. The aim of the present study was to evaluate in Balb/c mice the in vivo influence of orally administered Aloe vera drinking gel on the syngeneic L-1 sarcoma tumor growth and its vascularization: early cutaneous neovascular response, tumor-induced angiogenesis (TIA test read after 3 days), and tumor hemoglobin content measured 14 days after L-1 sarcoma cell grafting. Feeding mice for 3 days after tumor cell grafting with 150 μl daily dose of Aloe vera gel significantly diminished the number of newly-formed blood vessels in comparison to the controls. The difference between the groups of control and Aloe-fed mice (150 μl daily dose for 14 days) with respect to the 14 days' tumor volume was on the border of statistical significance. No difference was observed in tumor hemoglobin content.

  15. Lipoma arborescens of the subdeltoid bursa: a case report.

    Science.gov (United States)

    Teusink, Matthew; El-Khoury, Georges; Buckwalter, Joseph

    2010-01-01

    Lipoma arborescens is a benign, diffuse villous proliferation of the synovium characterized by replacement of the subsynovial tissue by mature adipocytes. Its etiology is unknown and fewer than 100 cases have been reported. It resembles other collections of subsynovial fat, the only difference being its large size and villous macroscopic appearance. It typically presents in patients in their fifth through seventh decades of life. It is most commonly monoarticular and most frequently affects the suprapatellar pouch of the knee. There have been reports of involvement of the hip, shoulder, wrist, elbow, ankle, and associated bursae. To our knowledge there have been only three previous cases of lipoma arborescens of the subdeltoid bursa in the literature. We report on a case of unilateral lipoma arborescens of the subdeltoid bursa in an elderly patient presenting as a shoulder mass.

  16. Sesquiterpenes and diterpenes from Ambrosia arborescens.

    Science.gov (United States)

    De Leo, Marinella; Saltos, Mariela Beatriz Vera; Puente, Blanca Fabiola Naranjo; De Tommasi, Nunziatina; Braca, Alessandra

    2010-05-01

    Six compounds, eudesm-11(13)-en-4beta,9beta-diol, 15R,16-dihydroxy-3-oxoisopimar-9(11)-ene, 15S,16-dihydroxy-3-oxoisopimar-9(11)-ene, 1alpha-hydroxy-7-oxo-iso-anhydrooplopanone, 10alpha-hydroxy-11,13-dihydro-5-epi-psilostachyin, and 4beta-hydroxypseudoguaian-12,6-olide 4-O-beta-d-glucopyranoside, together with 12 known sesquiterpenes, were isolated from the leaves of Ambrosia arborescens. Structures were elucidated by 1D and 2D NMR spectroscopy including 1D-TOCSY, DQF-COSY, 2D-ROESY, HSQC, and HMBC experiments, as well as by ESI mass spectrometry. The absolute configuration of the 15,16-diol moiety in 15R,16-dihydroxy-3-oxoisopimar-9(11)-ene and 15S,16-dihydroxy-3-oxoisopimar-9(11)-ene was determined using Snatzke's method. All compounds were evaluated for antiproliferative activity. Copyright 2010 Elsevier Ltd. All rights reserved.

  17. An in vivo and in vitro investigation of the effect of Aloe vera gel ethanolic extract using animal model with diabetic foot ulcer

    Directory of Open Access Journals (Sweden)

    Mohan Daburkar

    2014-01-01

    Full Text Available Aim: To examine the preventive effect of Aloe vera gel ethanolic extract using diabetic foot ulcer (DFUs protocol in Wistar rats. Materials and Methods: Male Wistar rats were divided into untreated control (Group I, untreated DFUs (Group II, DFUs treated with A. vera gel ethanolic extract (Group III, DFUs treated with topical A. vera gel (Group IV, DFUs treated with A. vera gel ethanolic extract and topical A. vera gel (Group V. The rats in the treatment groups were daily administered the A. vera gel and ethanolic extract for 9 days. Fasting blood glucose levels and percentage of wound ulcer contraction were measured on day 3, 6, and 9. Statistical Analysis used: The results are expressed as a mean ± Standard Error Mean (SEM. Data were analyzed using one-way analysis of variance (ANOVA after Newman-Keuls test. P < 0.05 were considered statistically significant in all cases. Results: Oral administration of A. vera gel ethanolic extract at a dose of 300 mg/kg body weight per day to diabetic rats for a period of 9 days resulted in a significant reduction in fasting blood glucose and a significant improvement in plasma insulin. Topical application of A. vera gel at a dose 30 mg/kg body weight per day to streptozotocin (STZ-induced diabetic rats for a period of 9 days resulted in no change in blood glucose and plasma insulin. Oral administration as well as topical application of A. vera gel ethanolic extract and gel significantly reduced the blood glucose, improved the plasma insulin, and significantly increased DNA and glycosaminoglycans (GAGs to improve the wound ulcer healing as well as the breaking strength on day 9. Conclusions: Present findings provide a scientific rationale for the use of A. vera gel ethanolic extract, and showed that the gel attenuated the diabetic foot wound in rats.

  18. Comparative evaluation of antimicrobial activity of hydroalcoholic extract of Aloe vera, garlic, and 5% sodium hypochlorite as root canal irrigants against Enterococcus faecalis: An in vitro study.

    Science.gov (United States)

    Karkare, Swati Ramesh; Ahire, Nivedita Pramod; Khedkar, Smita Uday

    2015-01-01

    Enterococcus faecalis are the most resistant and predominant microorganisms recovered from root canals of teeth where previous treatment has failed. Over the past decade, interest in drugs derived from medicinal plants has markedly increased. In dentistry, phytomedicines has been used as an anti-inflammatory, antibiotic, analgesic, sedative, and also as an endodontic irrigant. In endodontics, because of the cytotoxic reactions of most of the commercial intracanal medicaments and their inability to eliminate bacteria completely from dentinal tubules, the trend is shifting toward use of biologic medication extracted from natural plants. To compare the antimicrobial efficacy of newer irrigating agents which would probably be as effective or more and at the same time less irritating to the tissues than sodium hypochlorite (NaOCl). The objective of this study was to compare the antimicrobial activity of saturated and diluted (1:1) hydroalcoholic extract of Aloe vera, garlic, and 5% NaOCl against E. faecalis using the commonly used agar diffusion method. Saturated hydroalcoholic extract of A. vera showed the highest zone of inhibition against E. faecalis. NaOCl, which is considered as gold standard, also showed higher zones of inhibition.

  19. Comparative evaluation of antimicrobial activity of hydroalcoholic extract of Aloe vera, garlic, and 5% sodium hypochlorite as root canal irrigants against Enterococcus faecalis: An in vitro study

    Directory of Open Access Journals (Sweden)

    Swati Ramesh Karkare

    2015-01-01

    Full Text Available Introduction: Enterococcus faecalis are the most resistant and predominant microorganisms recovered from root canals of teeth where previous treatment has failed. Over the past decade, interest in drugs derived from medicinal plants has markedly increased. In dentistry, phytomedicines has been used as an anti-inflammatory, antibiotic, analgesic, sedative, and also as an endodontic irrigant. In endodontics, because of the cytotoxic reactions of most of the commercial intracanal medicaments and their inability to eliminate bacteria completely from dentinal tubules, the trend is shifting toward use of biologic medication extracted from natural plants. Aim: To compare the antimicrobial efficacy of newer irrigating agents which would probably be as effective or more and at the same time less irritating to the tissues than sodium hypochlorite (NaOCl. The objective of this study was to compare the antimicrobial activity of saturated and diluted (1:1 hydroalcoholic extract of Aloe vera, garlic, and 5% NaOCl against E. faecalis using the commonly used agar diffusion method. Results: Saturated hydroalcoholic extract of A. vera showed the highest zone of inhibition against E. faecalis. NaOCl, which is considered as gold standard, also showed higher zones of inhibition.

  20. On the novel action of melanolysis by a leaf extract of Aloe vera and its active ingredient aloin, potent skin depigmenting agents.

    Science.gov (United States)

    Ali, Sharique A; Galgut, Jyoti M; Choudhary, Ram K

    2012-05-01

    The present study was carried out to investigate the effects of an Aloe vera leaf extract, along with its standard active ingredient aloin, on the isolated tail melanophores of Bufo melanostictus tadpoles, which are a type of disguised smooth muscle cells offering excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. It was found that the leaf extract of A. vera and its active ingredient aloin induced powerful, dose-dependent, physiologically significant melanin aggregating effects in the isolated tail melanophores of B. melanostictus similar to those of adrenaline per se. These preliminary findings clearly demonstrate that the extract of A. vera and its active ingredient aloin cause melanin aggregation leading to skin lightening via alpha adrenergic receptor stimulation. The present study opens new vistas for the use of A. vera regarding its clinical application as a new nontoxic melanolytic agent for the treatment of hyperpigmentation. Georg Thieme Verlag KG Stuttgart · New York.

  1. Cytotoxicity study of plant Aloe vera (Linn

    Directory of Open Access Journals (Sweden)

    Atul N Chandu

    2012-01-01

    Full Text Available Background: The objective of this study has been to evaluate the in-vitro antitumor activity of Aloe vera extract of in cultured B16F10 melanoma cell line by measuring cell viability using "Trypan blue exclusion assay" method. Aim: To find out such kind of anticancer drug which is a cheap, safe, less toxic, and more potent drug compared to chemotherapy drug. Materials and Methods: In-vitro antitumor activity cell culture1, drug treatment (standard and test extract and Trypan blue exclusion assay growth and viability test 1 were used. Treatment of Aloe vera extract against B16F10 melanoma cell line, in all concentration range, showed decrease in percent cell viability, as compared to that of negative when examined by "Trypan blue exclusion assay". Results: In overall variation of test samples, Aloe vera extract showed its best activity in the concentration of 300 μg/ml, which was approximately equal to the activity of standard drug doxorubicin. Evaluation of in-vitro antitumor activity revealed that Aloe vera extract exhibits good cytotoxic activity. The best cytotoxic activity by Aloe vera was shown at 200 μg/ml concentration. Conclusion: The study of cytoprotection against normal cells by micronucleus assay has shown that the herbal extracts have less toxic effects to the normal blood lymphocytes, as compared to that of standard anticancer drug.

  2. Extent of Use of Aloe vera Locally Extracted Products for Management of Ailments in Communities of Kitagata Sub-county in Sheema District, Western Uganda

    Science.gov (United States)

    Adams, Kamukama; Eliot, Twineomujuni; Gerald, Agaba

    2015-01-01

    Aloe vera is widely used locally in communities in Uganda as a medicinal plant. It is said to contain various nutrient substances and vitamins that have curative properties. It is said to heal a variety of diseases in various communities. However the extent of use of this potential medicinal plant in Uganda and the various ailments for which it is used and the treatment outcomes are not clearly established and documented. In this cross-sectional study, carried out in August 2012 in Kitagata sub-county in Sheema district in western Uganda, data was collected from 131 randomly selected adult respondents using an interviewer administered semi-structured questionnaire. Key informants interviews and focused group discussions were also carried out with purposively selected participants. Data were collected on social demographic characteristics, practices and beliefs about Aloe vera. The data were analyzed using Excel version 2007 and Epi_Info software. To get the proportion of the community that use Aloe vera, the number of respondents that use aloe vera was expressed as a percentage of the total number of respondents. It was found out that all the respondents (100%) know aloe vera plant, 96.1% think that it can cure and 84.7% have ever used it. 90.9% of the respondents believe that Aloe vera is effective in curing ailments. 82.9% of these strongly believe in Aloe vera’s effectiveness. The diseases reported included malaria (31%), wounds (23%), abdominal pains (16%) and skin diseases (9%) among others. It was significantly noted that all the participants who had ever used Aloe vera still believe in it. 92.0% respondents reported that they can recommend aloe vera to a friend or relative. Only one participant strongly disagrees that Aloe vera has any curative properties and has never used it. PMID:26855960

  3. Extent of Use of Aloe vera Locally Extracted Products for Management of Ailments in Communities of Kitagata Sub-county in Sheema District, Western Uganda.

    Science.gov (United States)

    Adams, Kamukama; Eliot, Twineomujuni; Gerald, Agaba

    Aloe vera is widely used locally in communities in Uganda as a medicinal plant. It is said to contain various nutrient substances and vitamins that have curative properties. It is said to heal a variety of diseases in various communities. However the extent of use of this potential medicinal plant in Uganda and the various ailments for which it is used and the treatment outcomes are not clearly established and documented. In this cross-sectional study, carried out in August 2012 in Kitagata sub-county in Sheema district in western Uganda, data was collected from 131 randomly selected adult respondents using an interviewer administered semi-structured questionnaire. Key informants interviews and focused group discussions were also carried out with purposively selected participants. Data were collected on social demographic characteristics, practices and beliefs about Aloe vera. The data were analyzed using Excel version 2007 and Epi_Info software. To get the proportion of the community that use Aloe vera, the number of respondents that use aloe vera was expressed as a percentage of the total number of respondents. It was found out that all the respondents (100%) know aloe vera plant, 96.1% think that it can cure and 84.7% have ever used it. 90.9% of the respondents believe that Aloe vera is effective in curing ailments. 82.9% of these strongly believe in Aloe vera's effectiveness. The diseases reported included malaria (31%), wounds (23%), abdominal pains (16%) and skin diseases (9%) among others. It was significantly noted that all the participants who had ever used Aloe vera still believe in it. 92.0% respondents reported that they can recommend aloe vera to a friend or relative. Only one participant strongly disagrees that Aloe vera has any curative properties and has never used it.

  4. Facile one step synthesis of novel TiO2 nanocoral by sol-gel method using Aloe vera plant extract

    Science.gov (United States)

    Venkatesh, K. S.; Krishnamoorthi, S. R.; Palani, N. S.; Thirumal, V.; Jose, Sujin P.; Wang, Fu-Ming; Ilangovan, R.

    2015-05-01

    Titanium oxide (TiO2) nanoparticles (NPs) were synthesized by sol gel method using Aloe vera plant extract as a biological capping agent and a cauliflower-nanocoral morphology was observed in this technique. The assynthesized TiO2 nanopowder was calcined at a range of temperatures (300-600 °C) for 1 h. The influence of A. vera plant extract on the thermal, structural and morphological properties of TiO2 nanopowder was evaluated. Thermogravimetric analysis/differential thermal analysis was employed to study the thermal properties of the assynthesized TiO2 nanopowder. The crystallinity, phase transformation and the crystallite size of the calcined samples were studied by X-ray diffraction technique. XRD result confirmed the presence of TiO2 with anatase phase. FT Raman spectra showed the Raman active modes pertaining to the TiO2 anatase phase and Raman band shift was also observed with respect to particle size variation. The different functional group vibrations of as dried pure A. vera plant extract were compared with the mixture of TiO2 and A. vera plant extract by FT-IR analysis. The scanning electron microscopy images apparently showed the formation of spherical shaped NPs and also it demonstrated the effect of A. vera plant extract on the reduction of particles size. The surface area of the TiO2 NPs was measured through Brunauer-Emmett-Teller analysis. Transmission electron microscopy images ascertained that the spherical shaped TiO2 NPs were formed with cauliflower-nanocoral morphology decorated with nanopolyps with the size range between 15 and 30 nm.

  5. Mosquitocidal and water purification properties of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus leaf extracts against the mosquito vectors.

    Science.gov (United States)

    Arjunan, Nareshkumar; Murugan, Kadarkarai; Madhiyazhagan, Pari; Kovendan, Kalimuthu; Prasannakumar, Kanagarajan; Thangamani, Sundaram; Barnard, Donald R

    2012-04-01

    Ethanolic extracts of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus were tested for their toxicity effect on the third-instar larvae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. The leaves of C. dactylon, A. vera, H. indicus and C. amboinicus were collected from natural habitats (forests) in Western Ghats, Tamil Nadu, India. A total of 250 g of fresh, mature leaves were rinsed with distilled water and dried in shade. The dried leaves were put in Soxhlet apparatus and extract prepared using 100% ethanol for 72 h at 30-40°C. Dried residues were obtained from 100 g of extract evaporated to dryness in rotary vacuum evaporator. Larvicidal properties of ethanolic leaf extracts showed that the extracts are effective as mosquito control agents. The larval mortality was observed after 24 h exposure. No mortality was observed in the control. The median lethal concentration (LC(50)) values observed for the larvicidal activities are 0.44%, 0.51%, 0.59% and 0.68% for extracts of C. dactylon, A. vera, H. indicus and C. amboinicus, respectively. The observed mortality were statistically significant at P < 0.05 level. C. dactylon showed the highest mortality rate against the three species of mosquito larvae in laboratory and field. The selected plants were shown to exhibit water purification properties. Water quality parameters such as turbidity, pH and water clarity were analyzed in the water samples (pre-treatment and post-treatment of plant extracts) taken from the different breeding sites of mosquitoes. Water colour, turbidity and pH were reduced significantly after treatment with C. dactylon (13 HU, 31.5 mg/l and 6.9), H. indicus (13.8 HU, 33 mg/l and 7.1), A. vera (16 HU, 33.8 mg/l and 7.4) and C. amboinicus (21 HU, 35 mg/l and 7.5) extracts. The study proved that the extracts of C. dactylon, A. vera, H. indicus and C. amboinicus have both mosquitocidal and water sedimentation properties.

  6. A Pilot Study of the Effect of Aloe barbadensis Mill. Extract (AVH200®) in Patients with Irritable Bowel Syndrome: a Randomized, Double-Blind, Placebo-Controlled Study.

    Science.gov (United States)

    Størsrud, Stine; Pontén, Irina; Simrén, Magnus

    2015-09-01

    Few effective treatment options exist for patients with irritable bowel syndrome (IBS), and many patients state the use of aloe vera products reduce their symptoms. The aim of this pilot study was to investigate the effect of Aloe barbadensis Mill. Extract (AVH200®) in adult patients with IBS in a randomized, double-blind, placebo controlled study. Sixty-eight adult patients diagnosed with IBS according to the Rome III criteria were randomized to receive AVH200® or matching placebo for four weeks. Symptom questionnaires were completed on a weekly basis and the patients were asked if they had had adequate relief of their gastrointestinal symptoms. A tendency towards a higher proportion of responders in the aloe vera group (55%) vs. placebo (31%), (p=0.09) was observed, and the proportion of subjects who reported adequate relief at least 50% of the weeks during the treatment period tended to be larger in the aloe vera vs. placebo group (33% vs. 14%; p=0.12). The overall severity of the gastrointestinal symptoms was reduced in the aloe vera group (314+/-83 vs. 257+/-107; p=0.003) but not the placebo group (276+/-88 vs. 253+/-100; NS), without difference between the groups (p=0.10). AVH200® was well tolerated and no serious adverse events were observed. Even though the primary endpoint was not met, AVH200® seems to be a promising treatment option for patients with IBS owing to the positive results seen within the secondary endpoints. This study may have been underpowered to detect a clinically meaningful difference between the treatment groups, and therefore larger randomized, controlled studies are required to confirm these results and to elucidate potential mechanisms explaining its effect.

  7. Medicinal Mascarene Aloes: An audit of their phytotherapeutic potential.

    Science.gov (United States)

    Lobine, D; Cummins, I; Govinden-Soulange, J; Ranghoo-Sanmukhiya, M; Lindsey, K; Chazot, P L; Ambler, C A; Grellscheid, S; Sharples, G; Lall, N; Lambrechts, I A; Lavergne, C; Howes, M-J R

    2018-01-01

    A phytochemical and biological investigation of the endemic Mascarene Aloes (Aloe spp.), including A. tormentorii (Marais) L.E.Newton & G.D.Rowley, A. purpurea Lam, A. macra Haw., A. lomatophylloides Balf.f and A. vera (synonym A. barbadensis Mill.), which are used in the traditional folk medicine of the Mascarene Islands, was initiated. Methanolic extracts of the Aloes under study were analysed using high resolution LC-UV-MS/MS and compounds belonging to the class of anthraquinones, anthrones, chromones and flavone C-glycosides were detected. The Mascarene Aloes could be distinguished from A. vera by the absence of 2″-O-feruloylaloesin and 7-O-methylaloeresin. GC-MS analysis of monosaccharides revealed the presence of arabinose, fucose, xylose, mannose and galactose in all the Mascarene Aloes and in A. vera. The crude extracts of all Aloes analysed displayed antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Only extracts of A. macra were active against P. aeruginosa and Klebsiella pneumoniae, while none of the Aloe extracts inhibited Propionibacterium acnes. A. macra displayed anti-tyrosinase activity, exhibiting 50% inhibition at 0.95mg/mL, and extracts of A. purpurea (Mauritius) and A. vera displayed activity in a wound healing-scratch assay. In vitro cytotoxicity screening of crude methanolic extracts of the Aloes, using the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) showed that only A. purpurea (Réunion) elicited a modest toxic effect against HL60 cells, with a percentage toxicity of 8.2% (A. purpurea-Réunion) and none of the Aloe extracts elicited a toxic effect against MRC 5 fibroblast cells at a concentration of 0.1mg/mL. Mascarene Aloe species possess noteworthy pharmacological attributes associated with their rich phytochemical profiles. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. THE EFFECT OF ALOE VERA ON GASTRIC ACID SECRETION ...

    African Journals Online (AJOL)

    The effect of varying doses of ethanol extract of Aloe vera (Liliaceae) on acute gastric mucosal lesions induced by 0.6M HCl and acid output was studied in the pylorus ligated and lumen perfuse rats respectively. Acid secretion was determined by titration of the collected gastric juice to pH 7.0. Intraperitoneal injection of Aloe ...

  9. Antimutagenic and potential anticarcinogenic activities of aloe-vera ...

    African Journals Online (AJOL)

    The study was carried out to verify the potential anticarcinogenic and antimutagenic activity of garlic and aloe-vera. The ability of aqueous garlic extract and Aloe-Vera gel to inhibit mutation in tester strain of Escherichia coli WP2 uvrA was determined in this study. (The tester E. coli tryptophan auxotroph strain was obtained ...

  10. Green route for preparing extra-small TiO2 nanoparticles using aloe extracts and their application in degradation of methyl orange

    Science.gov (United States)

    Yin, Fang; Jiang, Bo; Chen, Chunfeng; Fu, Yunzhi; Chen, Yongjun; Xue, Xinghua; Mazidi, Mohsen

    2015-01-01

    TiO2 nanoparticles (NPs) with high-photocatalysis properties both in ultraviolet and visible-light regions were synthesized via a simple sol-gel method, and an aloe leaf extract (ALE) was used as the cap. When the Ti)∶)CHOH∶ALE volume ratio was equal to 0.5∶8∶40, the reaction time was 6 h, and crystallization occurred at 80°C for 2 h, the TiO2NPs had been synthesized. XRD analysis showed that the structure of TiO2NPs without thermal treatment was anatase. Transmission electron microscopy images confirmed their average size, which was around 5 nm. The products showed an excellent photocatalytic property for methyl orange in the presence of UV and sunlight with the characterization of UV-vis. The photocatalytic activity was optimized by adjusting the amount of TiO2, the pH value, and the calcination temperature. Results indicated that ALE acted as a cap and nano-TiO2 could be synthesized without calcination.

  11. EXAFS analysis of cations distribution in structure of Co1-xNixFe2O4 nanoparticles obtained by hydrothermal method in aloe vera extract solution

    Science.gov (United States)

    Wongpratat, Unchista; Maensiri, Santi; Swatsitang, Ekaphan

    2016-09-01

    Effect of cations distribution upon EXAFS analysis on magnetic properties of Co1-xNixFe2O4 (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles prepared by the hydrothermal method in aloe vera extract solution were studied. XRD analysis confirmed a pure phase of cubic spinel ferrite of all samples. Changes in lattice parameter and particle size depended on the Ni content with partial substitution and site distributions of Co2+, Ni2+ ions of different ionic radii at both tetrahedral and octahedral sites in the crystal structure. Particle sizes of samples estimated by TEM images were found to be in the range of 10.87-62.50 nm. The VSM results at room temperature indicated the ferrimagnetic behavior of all samples. Superparamagnetic behavior was observed in NiFe2O4 sample. The coercivity (Hc) and remanance (Mr) values were related to the particle sizes of samples. The saturation magnetization (Ms) was increased by a factor of 1.4 to a value of 57.57 emu/g, whereas the coercivity (Hc) was decreased by a factor of 20 to a value of 63.15 Oe for a sample with x = 0.75. In addition to the cations distribution, the increase of aspect ratio (surface to volume ratio) due to the decrease of particle size could significantly affect the magnetic properties of the materials.

  12. Phytochemical constituents and in vitro radical scavenging activity of different Aloe species.

    Science.gov (United States)

    Lucini, Luigi; Pellizzoni, Marco; Pellegrino, Roberto; Molinari, Gian Pietro; Colla, Giuseppe

    2015-03-01

    The phytochemical profile of Aloe barbadensis Mill. and Aloe arborescens Mill. was investigated using colorimetric assays, triple quadrupole and time-of-flight mass spectrometry, focusing on phenolic secondary metabolites in the different leaf portions. Hydroxycinnamic acids, several characteristic anthrones and chromones, the phenolic dimer feralolide and flavonoids such as flavones and isoflavones were identified. The stable radical DPPH test and the ORAC assay were then used to determine the in vitro radical scavenging. The outer green rind was the most active, while the inner parenchyma was much less effective. The 5-methylchromones aloesin, aloeresin A and aloesone were the most active among the pure secondary metabolites tested. The results suggest that several compounds are likely to contribute to the overall radical scavenging activity, and indicate that leaf portion must be taken into account when the plant is used for its antioxidant properties. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. Aloe vera: A review of toxicity and adverse clinical effects.

    Science.gov (United States)

    Guo, Xiaoqing; Mei, Nan

    2016-04-02

    The Aloe plant is employed as a dietary supplement in a variety of foods and as an ingredient in cosmetic products. The widespread human exposure and its potential toxic and carcinogenic activities raise safety concerns. Chemical analysis reveals that the Aloe plant contains various polysaccharides and phenolic chemicals, notably anthraquinones. Ingestion of Aloe preparations is associated with diarrhea, hypokalemia, pseudomelanosis coli, kidney failure, as well as phototoxicity and hypersensitive reactions. Recently, Aloe vera whole leaf extract showed clear evidence of carcinogenic activity in rats, and was classified by the International Agency for Research on Cancer as a possible human carcinogen (Group 2B). This review presents updated information on the toxicological effects, including the cytotoxicity, genotoxicity, carcinogenicity, and adverse clinical effects of Aloe vera whole leaf extract, gel, and latex.

  14. Biotransformation of aloenin, a bitter glucoside constituent of Aloe arborescens, by rats.

    Science.gov (United States)

    Hirata, T; Sakano, S; Suga, T

    1981-12-15

    Aloenin has been established to be 4-methoxy-6-(2-beta-D-glucopyranosyloxy-4-hydroxy-6-methylphenyl)-2-pyrone; it shows an inhibitory activity for gastric juice secretion. Rats metabolized it to 4-methoxy-6-(2,4-dihydroxy-6-methylphenyl)-2-pyrone, 2,5-dimethyl-7-hydroxychromone and glucose, which were excreted in the feces and the urine. The distribution of the radioactivity originating from 14C-labeled aloenin was studied. The tracer found in the kidney and the liver reached 60% of the amount administered 24 h after feeding and decreased rapidly in the next 24 h.

  15. Antiplasmodial potential and quantification of aloin and aloe-emodin in Aloe vera collected from different climatic regions of India.

    Science.gov (United States)

    Kumar, Sandeep; Yadav, Manila; Yadav, Amita; Rohilla, Pooja; Yadav, Jaya Parkash

    2017-07-17

    In this study, Aloe vera samples were collected from different climatic regions of India. Quantitative HPTLC (high performance thin layer chromatography) analysis of important anthraquinones aloin and aloe-emodin and antiplasmodial activity of crude aqueous extracts was done to estimate the effects of these constituents on antiplasmodial potential of the plant. HPTLC system equipped with a sample applicator Linomat V with CAMAG sample syringe, twin rough plate development chamber (20 x 10 cm), TLC Scanner 3 and integration software WINCATS 1.4.8 was used for analysis of aloin and aloe-emodin amount. The antiplasmodial activity of plant extracts was assessed against a chloroquine (CQ) sensitive strain of P. falciparum (MRC-2). Minimum Inhibitory Concentration (MIC) of aqueous extracts of selected samples was determined according to the World Health Organization (WHO) recommended method that was based on assessing the inhibition of schizont maturation in a 96-well microtitre plate. EC (effective concentration) values of different samples were observed to predict antiplasmodial potential of the plant in terms of their climatic zones. A maximum quantity of aloin and aloe-emodin i.e. 0.45 and 0.27 mg/g respectively was observed from the 12 samples of Aloe vera. The inhibited parasite growth with EC50 values ranging from 0.289 to 1056 μg/ml. The antiplasmodial EC50 value of positive control Chloroquine was observed 0.034 μg/ml and EC50 values showed by aloin and aloe-emodin was 67 μg/ml and 22 μg/ml respectively. A positive correlation was reported between aloin and aloe-emodin. Antiplasmodial activity was increased with increase in the concentration of aloin and aloe-emodin. The quantity of aloin and aloe-emodin was decreased with rise in temperature hence it was negatively correlated with temperature. The extracts of Aloe vera collected from colder climatic regions showed good antiplasmodial activity and also showed the presence of higher amount of aloin and aloe

  16. Irradiated vegetal extracts of mormodica charantia and cymbopogom nardus for the control of Blatella germanica; Extratos vegetais irradiados de mormodica charantia e cymbopogom nardus no controle de Blatella germanica

    Energy Technology Data Exchange (ETDEWEB)

    Nahme, Ligia Cardoso; Villavicencio, Anna Lucia C.H. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Centro de Tecnologia das Radiacoes. Lab. de Analise e Deteccao de Alimentos]. E-mail: lignah@hotmail.com; villavic@net.ipen.br; Potenza, Marcos Roberto Potenza [Instituto Biologico de Sao Paulo, SP (Brazil). Centro de Sanidade Vegetal. Lab. de Protecao e Clinica Vegetal

    2000-07-01

    Pertaining to one of the groups oldest of the world; the cockroach possess a great adaptive capacity and if they find gifts in diverse environments, being usually found in residences; independent if conserved well. Besides causing infestations, the cockroach cause serious riots and illnesses, therefore they act as vectors of pathogenic agents. Had to these events the interest to control these insects it magnified. One of the control forms oldest that come very being used; they are the vegetal extracts, in order to identify alternative products that substitute the conventional insecticides and that they do not cause problems to the environment. In the experiment cool parts of the plants had been used (leaves and flowers), that they had been triturated and homogenized in the ratio of 1:9 distilled water parts. The gotten mass was filtered in paper filter and solution gotten stored in freezer for posterior use. The radiated botanical species in the watery extract form had been Aloe arborescens, Delonix regia, Hemerocalis flaval, in the doses of 0 kGy, 2,5 kGy, 5 kGy and 10 kGy. The vegetal extract soon was supplied to the insects through the alimentary diet (feline ration), which was dived during one minute in the respective extract and supplied to the insects after the drying in ambient temperature. The used cockroach met in nymphs of and stadium of development and confined in plastic containers of 10 cm of re-covered diameter and 8 cm of height with teladas covers. The evaluations had elapsed during 7 days, where they had been gotten resulted with 12% of efficiency in the Aloe arborescens species and no result in Hemerocalis flaval.

  17. In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel.

    Science.gov (United States)

    Kang, Min-Cheol; Kim, Seo Young; Kim, Yoon Taek; Kim, Eun-A; Lee, Seung-Hong; Ko, Seok-Chun; Wijesinghe, W A J P; Samarakoon, Kalpa W; Kim, Young-Sun; Cho, Jin Hun; Jang, Hyeang-Su; Jeon, You-Jin

    2014-01-01

    The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Comparative evaluation of the antimicrobial activity of natural extracts of Morinda citrifolia, papain and aloe vera (all in gel formulation), 2% chlorhexidine gel and calcium hydroxide, against Enterococcus faecalis: An in vitro study

    Science.gov (United States)

    Bhardwaj, Anuj; Ballal, Suma; Velmurugan, Natanasabapathy

    2012-01-01

    Aim: A comparative evaluation of the antimicrobial activity of natural extracts of Morinda citrifolia, papain, and aloe vera (all in gel formulations), 2% chlorhexidine gel and calcium hydroxide, against Enterococcus faecalis—an in vitro study. Materials and Methods: The antimicrobial efficacy was assessed in vitro using dentin shavings collected at 2 depths of 200 and 400 μm. The total colony forming units at the end of 1, 3, and 5 days were assessed. Results: The overall percentage inhibition of bacterial growth (200 and 400 μm depth) was 100% with chlorhexidine gel. This was followed by M. citrifolia gel (86.02%), which showed better antimicrobial efficacy as compared with aloe vera gel (78.9%), papain gel (67.3%), and calcium hydroxide (64.3%). There was no statistical difference between data at 200 and 400 μm depth. Conclusion: Chlorhexidine gel showed the maximum antimicrobial activity against E. faecalis, whereas calcium hydroxide showed the least. Among the natural intracanal medicaments, M. citrifolia gel consistently exhibited good inhibition up to the 5th day followed by aloe vera gel and papain gel. PMID:22876022

  19. A simple aloe vera plant-extracted microwave and conventional combustion synthesis: Morphological, optical, magnetic and catalytic properties of CoFe2O4 nanostructures

    Science.gov (United States)

    Manikandan, A.; Sridhar, R.; Arul Antony, S.; Ramakrishna, Seeram

    2014-11-01

    Nanocrystalline magnetic spinel CoFe2O4 was synthesized by a simple microwave combustion method (MCM) using ferric nitrate, cobalt nitrate and Aloe vera plant extracted solution. For the comparative study, it was also prepared by a conventional combustion method (CCM). Powder X-ray diffraction, energy dispersive X-ray and selected-area electron diffraction results indicate that the as-synthesized samples have only single-phase spinel structure with high crystallinity and without the presence of other phase impurities. The crystal structure and morphology of the powders were revealed by high resolution scanning electron microscopy and transmission electron microscopy, show that the MCM products of CoFe2O4 samples contain sphere-like nanoparticles (SNPs), whereas the CCM method of samples consist of flake-like nanoplatelets (FNPs). The band gap of the samples was determined by UV-Visible diffuse reflectance and photoluminescence spectroscopy. The magnetization (Ms) results showed a ferromagnetic behavior of the CoFe2O4 nanostructures. The Ms value of CoFe2O4-SNPs is higher i.e. 77.62 emu/g than CoFe2O4-FNPs (25.46 emu/g). The higher Ms value of the sample suggest that the MCM technique is suitable for preparing high quality nanostructures for magnetic applications. Both the samples were successfully tested as catalysts for the conversion of benzyl alcohol. The resulting spinel ferrites were highly selective for the oxidation of benzyl alcohol and exhibit important difference among their activities. It was found that CoFe2O4-SNPs catalyst show the best performance, whereby 99.5% selectivity of benzaldehyde was achieved at close to 93.2% conversion.

  20. Synchronous Lipoma arborescens of bilateral wrist: An extremely rare manifestation and a new perspective on etiopathogenesis

    Directory of Open Access Journals (Sweden)

    Bal Chander

    2015-01-01

    Full Text Available Lipoma arborescens is a rare lesion wherein abnormal proliferation of adipocytes in the subsynovial region is seen. Whether it is neoplastic or not is still not entirely clear. Usually, Lipoma arborescens is seen unilaterally around knee joint but it can also be seen around tendon sheaths or bursa in adults. Multiple lesions are also reported. Involvement of wrist is extremely rare. We are presenting a case with bilateral wrist involvement by Lipoma arborescens which to the best of our knowledge is first one in English literature. There are a host of co-morbid/associated conditions reported with Lipoma arborescens out of which chronic irritation and inflammation appears to be most significant. We are proposing a novel perspective on etiopathogenesis of Lipoma arborescens based on extant understanding of cellular differentiation and information gleaned from fetal autopsies. The new perspective also explains the predilection for knee joint.

  1. Mosquitocidal and antibacterial activity of green-synthesized silver nanoparticles from Aloe vera extracts: towards an effective tool against the malaria vector Anopheles stephensi?

    Science.gov (United States)

    Dinesh, Devakumar; Murugan, Kadarkarai; Madhiyazhagan, Pari; Panneerselvam, Chellasamy; Kumar, Palanisamy Mahesh; Nicoletti, Marcello; Jiang, Wei; Benelli, Giovanni; Chandramohan, Balamurugan; Suresh, Udaiyan

    2015-04-01

    Mosquitoes represent an important threat for lives of millions of people worldwide, acting as vectors for devastating pathogens, such as malaria, yellow fever, dengue, and West Nile. In addition, pathogens and parasites polluting water also constitute a severe plague for populations of developing countries. Here, we investigated the mosquitocidal and antibacterial properties of Aloe vera leaf extract and silver nanoparticles synthesized using A. vera extract. Mosquitocidal properties were assessed in laboratory against larvae (I-IV instar) and pupae of the malaria vector Anopheles stephensi. Green-synthesized silver nanoparticles were tested against An. stephensi also in field conditions. Antibacterial properties of nanoparticles were evaluated against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi using the agar disk diffusion and minimum inhibitory concentration protocol. The synthesized silver nanoparticles were characterized by UV-vis spectrum, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). In laboratory conditions, the A. vera extract was toxic against An. stephensi larvae and pupae, even at low dosages. LC50 were 48.79 ppm (I instar), 59.09 ppm (II instar), 70.88 ppm (III instar), 83.58 ppm (IV instar), and 152.55 ppm (pupae). Green-synthesized silver nanoparticles were highly toxic against An. stephensi. LC50 were 3.825 ppm (I instar), 4.119 ppm (II instar), 4.982 ppm (III instar), 5.711 ppm (IV instar), and 6.113 ppm (pupae). In field conditions, the application of A. vera-synthesized silver nanoparticles (10 × LC50) leads to An. stephensi larval reduction of 74.5, 86.6, and 97.7%, after 24, 48, and 72 h, respectively. Nanoparticles also showed antibacterial properties, and the maximum concentration tested (150 mg/L) evoked an inhibition zone wider than 80 mm in all tested bacterium species. This study adds knowledge about the use of green synthesis of nanoparticles in

  2. Cytotoxic Compounds from Aloe megalacantha

    Directory of Open Access Journals (Sweden)

    Negera Abdissa

    2017-07-01

    Full Text Available Phytochemical investigation of the ethyl acetate extract of the roots of Aloe megalacantha led to the isolation of four new natural products—1,8-dimethoxynepodinol (1, aloesaponarin III (2, 10-O-methylchrysalodin (3 and methyl-26-O-feruloyl-oxyhexacosanate (4—along with ten known compounds. All purified metabolites were characterized by NMR, mass spectrometric analyses and comparison with literature data. The isolates were evaluated for their cytotoxic activity against a human cervix carcinoma cell line KB-3-1 and some of them exhibited good activity, with aloesaponarin II (IC50 = 0.98 µM being the most active compound.

  3. Aloe Vera in Dentistry

    Science.gov (United States)

    Kumar, G Senthil; Muruganandan, J; Prasad, T Srinivasa

    2014-01-01

    Aloe vera is a medicinal plant which has been used for thousands of years. The health benefits of aloe vera is well known and the dental uses of this plant is multiple. Interest is gathering among researchers regarding the use of this plant. Studies have proved the antiseptic, anti inflammatory, antiviral and antifungal properties of aloe vera and the use of this plant is proved beneficial. This plant is proved to be non allergic and very good in building up the immune system. Aloe vera is gaining popularity in dentistry as it is completely natural and there is no side effects being reported with its use. This paper gives an overview of the uses of this miracle plant and its uses in dentistry. PMID:25478478

  4. Aloe vera in dentistry.

    Science.gov (United States)

    Sujatha, G; Kumar, G Senthil; Muruganandan, J; Prasad, T Srinivasa

    2014-10-01

    Aloe vera is a medicinal plant which has been used for thousands of years. The health benefits of aloe vera is well known and the dental uses of this plant is multiple. Interest is gathering among researchers regarding the use of this plant. Studies have proved the antiseptic, anti inflammatory, antiviral and antifungal properties of aloe vera and the use of this plant is proved beneficial. This plant is proved to be non allergic and very good in building up the immune system. Aloe vera is gaining popularity in dentistry as it is completely natural and there is no side effects being reported with its use. This paper gives an overview of the uses of this miracle plant and its uses in dentistry.

  5. Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea.

    Science.gov (United States)

    Bisi-Johnson, Mary A; Obi, Chikwelu L; Samuel, Babatunde B; Eloff, Jacobus N; Okoh, Anthony I

    2017-06-19

    This study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the Eastern Cape Province, South Africa. The acetone extracts of Acacia mearnsii De Wild., Aloe arborescens Mill., A. striata Haw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa (Houtt.) Wehrh., Hermbstaedtia odorata (Burch. ex Moq.) T.Cooke, Hydnora africana Thunb, Hypoxis latifolia Wight, Pelargonium sidoides DC, Psidium guajava L and Schizocarphus nervosus (Burch.) van der Merwe were screened against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, multi-resistant Salmonella enterica serovar Isangi, S. typhi, S. enterica serovar Typhimurium, Shigella flexneri type 1b and Sh. sonnei phase II. A qualitative phytochemical screening of the plants extracts was by thin layer chromatography. Plants extracts were screened for antibacterial activity using serial dilution microplate technique and bioautography. The TLC fingerprint indicated the presence of terpenoids and flavonoids in the herbs. Most of the tested organisms were sensitive to the crude acetone extracts with minimum inhibitory concentration (MIC) values ranging from 0.018-2.5 mg/mℓ. Extracts of A. striata, C. uncinulata, E. autumnalis and P. guajava were more active against enteropathogens. S. aureus and Sh. flexneri were the most sensitive isolates to the crude extracts but of significance is the antibacterial activity of A. arborescens and P. guajava against a confirmed extended spectrum betalactamase positive S. enterica serovar Typhimurium. The presence of bioactive compounds and the antibacterial activity of some of the selected herbs against multidrug resistant enteric agents corroborate assertions by traditional healers on their efficacies.

  6. Extracting Features of Acacia Plantation and Natural Forest in the Mountainous Region of Sarawak, Malaysia by ALOS/AVNIR2 Image

    Science.gov (United States)

    Fadaei, H.; Ishii, R.; Suzuki, R.; Kendawang, J.

    2013-12-01

    The remote sensing technique has provided useful information to detect spatio-temporal changes in the land cover of tropical forests. Land cover characteristics derived from satellite image can be applied to the estimation of ecosystem services and biodiversity over an extensive area, and such land cover information would provide valuable information to global and local people to understand the significance of the tropical ecosystem. This study was conducted in the Acacia plantations and natural forest situated in the mountainous region which has different ecological characteristic from that in flat and low land area in Sarawak, Malaysia. The main objective of this study is to compare extract the characteristic of them by analyzing the ALOS/AVNIR2 images and ground truthing obtained by the forest survey. We implemented a ground-based forest survey at Aacia plantations and natural forest in the mountainous region in Sarawak, Malaysia in June, 2013 and acquired the forest structure data (tree height, diameter at breast height (DBH), crown diameter, tree spacing) and spectral reflectance data at the three sample plots of Acacia plantation that has 10 x 10m area. As for the spectral reflectance data, we measured the spectral reflectance of the end members of forest such as leaves, stems, road surface, and forest floor by the spectro-radiometer. Such forest structure and spectral data were incorporated into the image analysis by support vector machine (SVM) and object-base/texture analysis. Consequently, land covers on the AVNIR2 image were classified into three forest types (natural forest, oil palm plantation and acacia mangium plantation), then the characteristic of each category was examined. We additionally used the tree age data of acacia plantation for the classification. A unique feature was found in vegetation spectral reflectance of Acacia plantations. The curve of the spectral reflectance shows two peaks around 0.3μm and 0.6 - 0.8μm that can be assumed to

  7. MRI Findings of Synovial Lipoma Arborescens of the Hip: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hee Jin; Park, Chan Sub; Kim, Mi Sung; Park, Noh Hyuck; Park, Jai Hyung; Yi, Sang Yeop [Myoungji Hospital, Kwandong University College of Medicine, Seoul (Korea, Republic of); Park, Sung Il [Shinchon Severance Hospital, Yonsei University School of Medicine, Seoul (Korea, Republic of)

    2010-12-15

    Lipoma arborescens is a rare intra-articular lesion consisting of villous lipomatous proliferation of the synovium that tends to occur in the knee joint, especially in the suprapatellar pouch. The lesion has been observed in other locations, including the glenohumeral joint, subdeltoid bursa, hip, and elbow. We report a case of a lipoma arborescens of a unilateral hip joint in a 34-year-old man. MRI and a whole body bone scan were performed. In addition, a surgical synovectomy was performed and microscopic examination of the tumor revealed multiple variable-sized villous masses composed of mature fat tissue and lined by synovial cells which confirmed the diagnosis of lipoma arborescens

  8. Bilateral lipoma arborescens of the knee in a child: a case report.

    Science.gov (United States)

    Cil, Akin; Atay, O Ahmet; Aydingöz, Ustün; Tetik, Onur; Gedikoğlu, Gökhan; Doral, M Nedim

    2005-09-01

    We report a case of childhood lipoma arborescens of both knee joints who had been erroneously diagnosed to have initially acute rheumatic fever and subsequently oligoarticular juvenile rheumatoid arthritis. She had taken anti-inflammatory medication for 8 years without remission of the effusion. Magnetic resonance imaging (MRI) and synovectomy revealed the diagnosis of lipoma arborescens. Lipoma arborescens should be kept in mind in the differential diagnosis of refractory chronic joint effusion and synovial hypertrophy in the childhood period, and MRI yields the correct diagnosis in this setting.

  9. ACID HIDROLYSIS OF ALOE VERA (SABILA) BAGASSE FOR OBTAINING PECTIN

    OpenAIRE

    Caridad Curbelo Hernández; María Eugenia Moreno Quintero; Danibett Ramírez Hernández; Lourdes M. Crespo Zafra

    2017-01-01

    The processing of Aloe vera (sábila in sp.) has reached a great development during the last years, since its products are raw material for other industries. This has contributed to the increase of solid residues, which are not utilized to obtain added value products in the same industry. In this research, the acid hydrolysis method for pectin extraction from the Aloe vera bagasse was studied. Firstly, the bagasse was characterized determinig: humidity, ashes, proteins, acidity, and total cont...

  10. Aloes of the world project

    DEFF Research Database (Denmark)

    Klopper, Ronell R.; Smith, Gideon F.; Crouch, Neil R.

    2013-01-01

    Background - The Old World genus Aloe L. comprises ± 630 species to which almost 1300 names have been applied. Members of the genus are prominent components of many, mainly arid, African landscapes. Aloes can be found in Africa (the majority of species), the Arabian Peninsula, Socotra, Madagascar...... and the Mascarene Islands. The berried aloes of the genus Lomatophyllum Willd. are included as a section of the genus Aloe for the purpose of this project. Aims - As part of the African Plants Initiative (JSTOR Plant Science), the Aloes of the World project aims to provide a comprehensive electronic facility...

  11. [Relationship between antibacterial activity of aloe and its anthaquinone compounds].

    Science.gov (United States)

    Tian, Bing; Hua, Yue-jin; Ma, Xiao-qiong; Wang, Guan-lin

    2003-11-01

    To investigate the relationship between the antibacterial activity of aloe and its contents of anthaquinone compounds, measure and compale antibacterial activities of aloin and aloe-emodin, and analyse the effect of glycoside on the antibacterial activity of aloin. The antibacterial activities of the extracts from the outer leaf of Aloe saponaria Haw, aloin and aloe-emodin against three Gram-negative and two Gram-positive bacteria were investigated with the method of agar diffusion. The antibacterial effect of aloin on E. coli was further studied with scanning electron microscopy. The antibacterial activities of aloe showed to be dependent on the dose of anthraquinone, aloin (1 g x L(-1)) exhibited higher antibacterial activity [inhibition diameter > (7. 1 +/- 0.15) mm] than Aloe-emodin (inhibition diameter aloin changed the morphology of E. coli and damaged the outer cell structrue. Anthraquinone compounds are the active antibacterial components in aloe and aloin is the main active compound. The glycoside makes it easy for aloin to invade cells and enhances its activity.

  12. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

    OpenAIRE

    Ramachandran, Pushkala; Nagarajan, Srividya

    2014-01-01

    Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG) and papaya. Aloe gel (30%), papaya pulp (15%), spice extract (5%), and citric acid (0.1%) were mixed in given proportion to prepare...

  13. LIPOMA ARBORESCENS: RARE CASE OF ROTATOR CUFF TEAR ASSOCIATED WITH THE PRESENCE OF LIPOMA ARBORESCENS IN THE SUBACROMIAL-SUBDELTOID AND GLENOHUMERAL BURSA.

    Science.gov (United States)

    Benegas, Eduardo; Neto, Arnaldo Amado Ferreiro; Teodoro, Daniel Sabatini; da Silva, Marcos Vinícius Muriano; de Oliveira, Augusto Medaglia; Filippi, Renée Zon; de Santis Prada, Flávia

    2012-01-01

    Lipoma arborescens is a rare intra-articular disease that is usually monoarticular and is characterized by extensive proliferation of the synovial villi and hyperplasia of the subsynovial fat. The synovial tissue is progressively replaced by mature fat cells in the synovial membrane. The present study reports a case of a rare condition of lipoma arborescens that was simultaneously intra-articular (glenohumeral joint) and in the subacromial-subdeltoid bursa, in association with a torn supraspinatus tendon. The clinical, histological and radiographic presentations and treatment are discussed here. The description of this case includes radiographic and magnetic resonance evaluations and pathological examination. Although lipoma arborescens is a rare condition, it should be taken into consideration in cases presenting synovial hyperproliferation and synovial fat replacement.

  14. α-Mangostin from Cratoxylum arborescens: An in vitro and in vivo toxicological evaluation

    Directory of Open Access Journals (Sweden)

    Mohamed Yousif Ibrahim

    2015-01-01

    Full Text Available α-Mangostin (AM is believed to be beneficial for health due to its versatile biological activities such as antioxidant, antiviral and anticancer properties. In this study, the air-dried stem bark of Cratoxylum arborescens was extracted consecutively with hexane, chloroform and methanol. The hexane extract was chromatographed, fractionated and purified to yield AM. The toxic effects of AM have not been completely investigated; therefore, the current study investigates its in vitro cytotoxicity on WRL-68 normal cells and in vivo effects on renal and hepatic histobiochemical parameters, relative organ weight, lipid profile, peroxidation and reduced glutathione of ICR female and male mice. AM was fed orally at single doses of 0 (as normal group, 100, 500 and 1000 mg/kg body weight. The results showed that AM did not show adverse effects on body weight, organ weight, serum biochemistry, histopathology and oxidative stress biomarkers. On the other hand, this natural compound showed low cytotoxic activities against normal liver cells (WRL-68 with IC50 = 65 μg/ml. To our knowledge, this paper is the first to report the toxicity effects of AM in rodents.

  15. Influence of environmental parameters on mycotoxin production by Alternaria arborescens.

    Science.gov (United States)

    Vaquera, Sandra; Patriarca, Andrea; Fernández Pinto, V

    2016-02-16

    Alternaria arborescens has been reported as a common fungal species invading tomatoes and is capable of producing several mycotoxins in infected plants, fruits and in agricultural commodities. Alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA) are some of the main Alternaria mycotoxins that can be found as contaminants of food. This species can produce these toxic metabolites together with AAL toxins (Alternaria alternata f. sp. lycopersicum toxins), which can act as inhibitors of sphingolipid biosynthesis. The objective of this study was to determine the effect of water activity (aw, 0.995, 0.975, 0.950) and temperature (6, 15, 20, 25 and 30 °C) on mycotoxin production by A. arborescens on a synthetic tomato medium. The optimum production of AOH and AME occurred at 0.975 aw after 40 days of incubation at 30 °C. The maximum TeA accumulation was observed at 0.975 aw and 25 °C and at 0.950 aw and 30 °C. AAL TA was produced in higher quantities at 0.995 aw and 30 °C. At 6 °C no quantifiable levels of AOH or AME were detected, but significant amounts of TeA were produced at 0.975 aw. In general, high aw levels and high temperatures were favorable for mycotoxin production. The greatest accumulation of all four toxins occurred at 0.975 aw and 30 °C. The results obtained here could be extrapolated to evaluate the risk of tomato fruits and tomato products contamination caused by these toxins. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4 in rats Efeito de um extrato de Aloe vera na biodistribuição do pertecnetato de sódio (Na99mTcO4 em ratos

    Directory of Open Access Journals (Sweden)

    Cecília Maria de Carvalho Xavier Holanda

    2009-10-01

    Full Text Available PURPOSE: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na99mTcO4 and laboratorial parameters in Wistar rats. METHODS: Twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na99mTcO4 by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (%ATI/g was determined. Biochemical dosages were performed. RESULTS: There was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (pOBJETIVO: Aloe vera é uma planta tropical popularmente conhecida no Brasil por "babosa". Investigou-se o efeito de extrato aquoso do A. vera na biodistribuição do pertecnetato de sódio (Na99mTcO4 e em parâmetros laboratoriais de ratos Wistar. MÉTODOS: Doze animais foram divididos em 2 grupos: tratado e controle. No grupo tratado, o extrato de A. vera foi administrado via oral (5mg/mL/dia por 10 dias. O grupo controle recebeu sorbitol do mesmo modo. Uma hora após a última dose, ambos receberam 0,1mL de Na99mTcO4 via plexo orbital. Após 60 minutos, os animais foram sacrificados. Foram retiradas amostras do cérebro, fígado, coração, músculo, pâncreas, estômago, fêmur, rins, sangue, testículos e tiróide e determinou-se o percentual de radioatividade por grama (%ATI/g de cada uma. Dosagens bioquímicas foram realizadas. RESULTADOS: Houve

  17. Multiple anticancer effects of damsin and coronopilin isolated from Ambrosia arborescens on cell cultures.

    Science.gov (United States)

    Villagomez, Rodrigo; Rodrigo, Gloria C; Collado, Isidro G; Calzado, Marco A; Muñoz, Eduardo; Åkesson, Björn; Sterner, Olov; Almanza, Giovanna R; Duan, Rui-Dong

    2013-09-01

    Terpenoids in plants are important sources for drug discovery. In this study, we extracted damsin and coronopilin, two sesquiterpene lactones, from Ambrosia arborescens and examined their anticancer effects on cell cultures. Damsin and coronopilin inhibited cell proliferation, DNA biosynthesis and formation of cytoplasmic DNA histone complexes in Caco-2 cells, with damsin being more potent than coronopilin. Further studies using the luciferase reporter system showed that damsin and coronopilin also inhibited expressions of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription-3 (STAT3), indicating that these sesquiterpenes can interfere with NF-κB and STAT3 pathways. Finally, we examined the effects of two synthetic dibrominated derivatives of damsin, 11α,13-dibromodamsin and 11β,13-dibromodamsin. While bromination appeared to weaken the antiproliferative effects of damsin, the β epimer had strong inhibitory effects on STAT3 activation. In conclusion, the sesquiterpene lactones damsin and coronopilin have inhibitory effects on cell proliferation, DNA biosynthesis and NF-κB and STAT3 pathways, thus being potentially important for discovery of drugs against cancer.

  18. [Antimetastatic properties of aloe juice].

    Science.gov (United States)

    Gribel', N V; Pashinskiĭ, V G

    1986-01-01

    An evaluation of antimetastatic properties of succus Aloës was carried out using three types of experimental tumors of mice and rats. It was found that succus Aloës treatment contributes to reduction of tumor mass, metastatic foci and metastasis frequency at different stages of tumor progress without affecting major tumor growth. Succus Aloës potentiates the antitumor effect of 5-fluorouracil and cyclophosphamide as components of combination chemotherapy.

  19. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

    OpenAIRE

    Inder Sehgal; Winters, Wallace D.; Michael Scott; Andrew David; Glenn Gillis; Thaya Stoufflet; Anand Nair; Konstantine Kousoulas

    2013-01-01

    Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL) aloe vera, treated with activ...

  20. [GPC Fingerprint Chromatograms of Aloe vera Leaf Gel Polysaccharides].

    Science.gov (United States)

    Wang, Qiao-e; Xie, Dan; Qian, Jie; Dong, Yin-mao

    2015-10-01

    To establish the gel permeation chromatography (GPC) fingerprint chromatograms of polysaccharides in Aloe vera leaf gel from the same habitat (Beijing) and different habitats for evaluating the quality of Aloe vera leaf gel products commercially available and testing common adulterated substances. The samples were prepared by water-extraction and alcohol-precipitation method. GPC separation was performed on a Shodex SUGAR KS-805 (300 mm x 8.0 mm, 7 μm) column and a Shodex SUGAR KS-803 (300 mm x 8.0 mm, 6 μm) column at the temperature of 60 degrees C by eluting with 0.1 mol/L NaNO3 (containing 0.2 per thousand NaN) at a flow rate of 0.8 mL/min, the chromatographic effluent was detected by refractive index detector (RID) at the temperature of 50 degrees C. The common pattern of GPC fingerprint chromatograms was established and four common peaks were demarcated. The similarities of samples from the same habitat (Beijing) and different habitats were over 0.9. Taking the GPC fingerprint chromatograms for the qualified model, three commercially available aloe products were evaluated to be made of Aloe vera by the different manufacturing processes and four common adulterated substances of aloe polysaccharides were identified effectively. The method is simple and accurate with a good reproducibility, and it can be used for the identification and quality evaluation of Aloe vera leaf gel products.

  1. Design, formulation and evaluation of Aloe vera chewing gum.

    Science.gov (United States)

    Aslani, Abolfazl; Ghannadi, Alireza; Raddanipour, Razieh

    2015-01-01

    Aloe vera has antioxidant, antiinflammatory, healing, antiseptic, anticancer and antidiabetic effects. The aim of the present study was to design and evaluate the formulation of Aloe vera chewing gum with an appropriate taste and quality with the indications for healing oral wounds, such as lichen planus, mouth sores caused by cancer chemotherapy and mouth abscesses as well as reducing mouth dryness caused by chemotherapy. In Aloe vera powder, the carbohydrate content was determined according to mannose and phenolic compounds in terms of gallic acid. Aloe vera powder, sugar, liquid glucose, glycerin, sweeteners and different flavors were added to the soft gum bases. In Aloe vera chewing gum formulation, 10% of dried Aloe vera extract entered the gum base. Then the chewing gum was cut into pieces of suitable sizes. Weight uniformity, content uniformity, the organoleptic properties evaluation, releasing the active ingredient in the phosphate buffer (pH, 6.8) and taste evaluation were examined by Latin square method. One gram of Aloe vera powder contained 5.16 ± 0.25 mg/g of phenolic compounds and 104.63 ± 4.72 mg/g of carbohydrates. After making 16 Aloe vera chewing gum formulations, the F16 formulation was selected as the best formulation according to its physicochemical and organoleptic properties. In fact F16 formulation has suitable hardness, lack of adhesion to the tooth and appropriate size and taste; and after 30 min, it released more than 90% of its drug content. After assessments made, the F16 formulation with maltitol, aspartame and sugar sweeteners was selected as the best formulation. Among various flavors used, peppermint flavor which had the most acceptance between consumers was selected.

  2. Design, formulation and evaluation of Aloe vera chewing gum

    Science.gov (United States)

    Aslani, Abolfazl; Ghannadi, Alireza; Raddanipour, Razieh

    2015-01-01

    Background: Aloe vera has antioxidant, antiinflammatory, healing, antiseptic, anticancer and antidiabetic effects. The aim of the present study was to design and evaluate the formulation of Aloe vera chewing gum with an appropriate taste and quality with the indications for healing oral wounds, such as lichen planus, mouth sores caused by cancer chemotherapy and mouth abscesses as well as reducing mouth dryness caused by chemotherapy. Materials and Methods: In Aloe vera powder, the carbohydrate content was determined according to mannose and phenolic compounds in terms of gallic acid. Aloe vera powder, sugar, liquid glucose, glycerin, sweeteners and different flavors were added to the soft gum bases. In Aloe vera chewing gum formulation, 10% of dried Aloe vera extract entered the gum base. Then the chewing gum was cut into pieces of suitable sizes. Weight uniformity, content uniformity, the organoleptic properties evaluation, releasing the active ingredient in the phosphate buffer (pH, 6.8) and taste evaluation were examined by Latin square method. Results: One gram of Aloe vera powder contained 5.16 ± 0.25 mg/g of phenolic compounds and 104.63 ± 4.72 mg/g of carbohydrates. After making 16 Aloe vera chewing gum formulations, the F16 formulation was selected as the best formulation according to its physicochemical and organoleptic properties. In fact F16 formulation has suitable hardness, lack of adhesion to the tooth and appropriate size and taste; and after 30 min, it released more than 90% of its drug content. Conclusion: After assessments made, the F16 formulation with maltitol, aspartame and sugar sweeteners was selected as the best formulation. Among various flavors used, peppermint flavor which had the most acceptance between consumers was selected. PMID:26605214

  3. Lipoma arborescens arising in the extra-articular bursa of the knee joint

    Science.gov (United States)

    Minami, Shinji; Miyake, Yusuke; Kinoshita, Hirofumi

    2016-01-01

    Lipoma arborescens arising in the extra-articular bursa of the knee joint is extremely rare. We describe an 11-year-old boy who complained of a gradual swelling mass of the lateral knee joint. Magnetic resonance imaging (MRI) showed a high signal intensity tumor on T1- and T2-weighted images with a thickened septa and nodular lesion that showed low signal intensity. The radiologist suggested the possible differential diagnosis of well-differentiated liposarcoma. At operation, the tumor was found under the iliotibial tract and was not in contact with the knee joint. Histopathologically, this lesion was diagnosed as lipoma arborescens arising in the extra-articular bursa of the knee joint. On MRI, the appearance of lipoma arborescens arising in the extra-articular bursa of the knee joint differed from that of conventional intra-articular lipoma arborescens. In this report, we describe a case of extra-articular lipoma arborescens of the knee joint bursa and discuss the diagnosis and etiology. PMID:27382924

  4. Fungi associated with base rot disease of aloe vera (Aloe ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-12-17

    Dec 17, 2008 ... Aloe barbadensis Miller, popularly called Aloe vera is a phanerogame angiosperm which belongs to the family .... Illustrated genera of imperfect fungi,. Burgess Publishing Co., New York, p. 241. Barron GI (1968). The genera of Hyphomycetes from soil. Robert and. Krieger Publishing Co, New York, p. 364.

  5. Fungi associated with base rot disease of aloe vera ( Aloe ...

    African Journals Online (AJOL)

    Fungi associated with base rot disease of Aloe vera (syn. Aloe barbadensis) were investigated in Niger Delta Area of Nigeria. Fungi and their percentage frequency were Aspergillus verocosa 28.03%, Fusarium oxysporium 24.24%, Plectosphaerella cucumerina 16.67%, Mammeria ehinobotryoides 15.91% and Torula ...

  6. Protective effects of Aloe sterols against UVB-induced photoaging in hairless mice.

    Science.gov (United States)

    Misawa, Eriko; Tanaka, Miyuki; Saito, Marie; Nabeshima, Kazumi; Yao, Ruiqing; Yamauchi, Kouji; Abe, Fumiaki; Yamamoto, Yuki; Furukawa, Fukumi

    2017-03-01

    Aloe vera is a traditional medical plant whose gel has been widely used in skin care. Previously, we have identified Aloe sterols from Aloe vera as active ingredients. This study investigated the protective effects of Aloe sterols without polysaccharides, against ultraviolet B (UVB)-induced skin photoaging in mice using Aloe vera gel extract (AVGE) obtained by supercritical fluid extraction. Aloe vera gel extract was supplemented in the diet (12 or 120 ppm), and HR-1 hairless mice were exposed to UVB irradiation for 7 weeks. Skin measurements and histological and analytical studies were performed. Repeated UVB irradiation induced rough wrinkling of skin with water content reduction and hyperkeratosis. AVGE administration resulted in the significant improvement of UVB-induced skin dryness, epidermal thickness, and wrinkle formation. The AVGE group also suppressed the degenerations of dermal collagen fibers and the appearance of cutaneous apoptosis cells induced by UVB. Furthermore, AVGE administration reduced the excess elevation of pro-inflammatory cytokines (IL-1β and TNF-α) and matrix metalloproteinases (MMP-2, MMP-9, MMP-12, and MMP-13) in UVB-exposed skin. The dietary ingestion of Aloe sterols protected against chronic UVB damage in mouse skin, and our results suggest that Aloe sterols may prevent skin photoaging through the anti-inflammation and MMP regulation. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  7. Processing, food applications and safety of aloe vera products: a review.

    Science.gov (United States)

    Ahlawat, Kulveer Singh; Khatkar, Bhupender Singh

    2011-10-01

    Aloe vera is used for vigor, wellness and medicinal purposes since rigvedic times. Health benefits of aloe vera include its application in wound healing, treating burns, minimizing frost bite damage, protection against skin damage from x-rays, lung cancer, intestinal problems, increasing high density lipoprotein (HDL), reducing low density lipoprotein (LDL), reducing blood sugar in diabetics, fighting acquired immune deficiency syndrome (AIDS), allergies and improving immune system. Phytochemistry of aloe vera gel has revealed the presence of more than 200 bioactive chemicals. Aloe vera gel is extracted from its leaves and appropriate processing techniques are needed for stabilization as well as preparation of the end products. The industries involved in processing of aloe vera need Government surveillance to ensure that the aloe vera products have beneficial bio-active chemicals as per claims of the manufacturers. Regulatory bodies also need to look into the safety and toxicological aspects of aloe vera products for food applications. The claims made for medicinal value of aloe products should be supported by authentic and approved clinical trial data. It is presumptive to mention that nutraceutical claims of aloe products made by the manufacturers are numerous. However, approved clinical evidences are available only for lowering LDL, increasing HDL, decreasing blood glucose level, treating genital herpes and psoriases.

  8. Aloe vera: A short review

    Directory of Open Access Journals (Sweden)

    Surjushe Amar

    2008-01-01

    Full Text Available Aloe vera is a natural product that is now a day frequently used in the field of cosmetology. Though there are various indications for its use, controlled trials are needed to determine its real efficacy. The aloe vera plant, its properties, mechanism of action and clinical uses are briefly reviewed in this article.

  9. Lipoma arborescens: caso raro de ruptura do manguito rotador associado à presença de lipoma arborescens na bursa subacromial-subdeltoidea e glenoumeral Lipoma arborescens: rare case of rotator cuff tear associated with the presence of lipoma arborescens in the subacromial-subdeltoid and glenohumeral bursa

    Directory of Open Access Journals (Sweden)

    Eduardo Benegas

    2012-01-01

    Full Text Available Lipoma arborescens é uma condição rara de moléstia intra-articular, usualmente monoarticular, caracterizada por extensa proliferação dos vilos sinoviais e hiperplasia da gordura subsinovial. O tecido sinovial é progressivamente substituído por células maduras de gordura na membrana sinovial. O presente trabalho é o relato de caso de uma condição rara de lipoma arborescens tanto intra-articular (glenoumeral como da bursa subacromial-subdeltoide além de ruptura do tendão do supraespinhoso. As apresentações clínicas, histológicas e radiográficas assim como o tratamento são discutidos no presente estudo. A apresentação do caso contempla também a avaliação radiográfica, ressonância magnética e exame patológico. Apesar do lipoma arborescens ser uma condição rara, tal hipótese deve ser considerada frente a um caso com hiperproliferação sinovial e lipossubstituição da sinovial.Lipoma arborescens is a rare intra-articular disease that is usually monoarticular and is characterized by extensive proliferation of the synovial villi and hyperplasia of the subsynovial fat. The synovial tissue is progressively replaced by mature fat cells in the synovial membrane. The present study reports a case of a rare condition of lipoma arborescens that was simultaneously intra-articular (glenohumeral joint and in the subacromial-subdeltoid bursa, in association with a torn supraspinatus tendon. The clinical, histological and radiographic presentations and treatment are discussed here. The description of this case includes radiographic and magnetic resonance evaluations and pathological examination. Although lipoma arborescens is a rare condition, it should be taken into consideration in cases presenting synovial hyperproliferation and synovial fat replacement.

  10. Multi-responses extraction optimization combined with high-performance liquid chromatography-diode array detection-electrospray ionization-tandem mass spectrometry and chemometrics techniques for the fingerprint analysis of Aloe barbadensis Miller.

    Science.gov (United States)

    Zhong, Jia-Sheng; Wan, Jin-Zhi; Ding, Wen-Jing; Wu, Xiao-Fang; Xie, Zhi-Yong

    2015-03-25

    A quality control strategy using high-performance liquid chromatography-diode array detector-electrospray ionization-tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) coupled with chemometrics analysis was proposed for Aloe barbadensis Miller. Firstly, the extraction conditions including methanol concentration, extraction time and solvent-to-material ratio were optimized by multi-responses optimization based on response surface methodology (RSM). The optimum conditions were achieved by Derringer's desirability function and experimental validation implied that the established model exhibited favorable prediction ability. Then, HPLC fingerprint consisting of 27 common peaks was developed among 15 batches of A. barbadensis samples. 25 common peaks were identified using HPLC-DAD-ESI-MS/MS method by their spectral characteristics or comparison with the authentic standards. Chemometrics techniques including similarity analysis (SA), principal components analysis (PCA) and hierarchical clustering analysis (HCA) were implemented to classify A. barbadensis samples. The results demonstrated that all A. barbadensis samples shared similar chromatographic patterns as well as differences. These achievements provided an effective, reliable and comprehensive quality control method for A. barbadensis. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Quality control of Aloe vera beverages

    OpenAIRE

    Lachenmeier, Katrin; Kuepper, Uta; Musshoff, Frank; Madea, Burkhard; Reusch, Helmut; Lachenmeier, Dirk W.

    2005-01-01

    Aloe vera beverages have to be produced exclusively using material of the plant species Aloe barbadensis MILLER. Commercial material was reported to be frequently adulterated by artificial preservatives or to lack significant amounts of Aloe ingredients. HPTLC and HS-SPME/GC/MS methods to assess the authenticity of Aloe vera beverages were developed in this study, allowing to differentiate between authentic and adulterated products. In one case a commercially available Aloe vera juice could b...

  12. Nanocrystalline spinel ferrite (MFe{sub 2}O{sub 4}, M = Ni, Co, Mn, Mg, Zn) powders prepared by a simple aloe vera plant-extracted solution hydrothermal route

    Energy Technology Data Exchange (ETDEWEB)

    Phumying, Santi; Labuayai, Sarawuth; Swatsitang, Ekaphan; Amornkitbamrung, Vittaya [Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Integrated Nanotechnology Research Center (INRC), Khon Kaen University, Khon Kaen 40002 (Thailand); Maensiri, Santi, E-mail: santimaensiri@gmail.com [School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000 (Thailand)

    2013-06-01

    Graphical abstract: This figure shows the specific magnetization curves of the as-prepared MFe{sub 2}O{sub 4} (M = Ni, Co, Mn, Mg, Zn) powders obtained from room temperature VSM measurement. These curves are typical for a soft magnetic material and indicate hysteresis ferromagnetism in the field ranges of ±500 Oe, ±1000 Oe, and ±2000 Oe for the CoFe{sub 2}O{sub 4}, MgFe{sub 2}O{sub 4} and MnFe{sub 2}O{sub 4} respectively, whereas the samples of NiFe{sub 2}O{sub 4} and ZnFe{sub 2}O{sub 4} show a superparamagnetic behavior. Highlights: ► Nanocrystalline MFe{sub 2}O{sub 4} powders were synthesized by a novel hydrothermal method. ► Metal acetylacetonates and aloe vera plant-extracted solution are used. ► This biosynthetic route is very simple and provides high-yield oxide nanomaterials. ► XRD and TEM results indicate that the prepared samples have only spinel structure. ► The maximum M{sub s} of 68.9 emu/g at 10 kOe were observed for the samples of MnFe{sub 2}O{sub 4}. - Abstract: Nanocrystalline spinel ferrite MFe{sub 2}O{sub 4} (M = Ni, Co, Mn, Mg, Zn) powders were synthesized by a novel hydrothermal method using Fe(acac){sub 3}, M(acac){sub 3} (M = Ni, Co, Mn, Mg, Zn) and aloe vera plant extracted solution. The X-ray diffraction and selected-area electron diffraction results indicate that the synthesized nanocrystalline have only spinel structure without the presence of other phase impurities. The crystal structure and morphology of the spinel ferrite powders, as revealed by TEM, show that the NiFe{sub 2}O{sub 4} and CoFe{sub 2}O{sub 4} samples contain nanoparticles, whereas the MnFe{sub 2}O{sub 4} and MgFe{sub 2}O{sub 4} samples consist of many nanoplatelets and nanoparticles. Interestingly, the ZnFe{sub 2}O{sub 4} sample contains plate-like structure of networked nanocrystalline particles. Room temperature magnetization results show a ferromagnetic behavior of the CoFe{sub 2}O{sub 4}, MnFe{sub 2}O{sub 4} and MgFe{sub 2}O{sub 4} samples, whereas the

  13. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)

    Extraction of the yellow colour compounds of leaves of Aloe excelsa were performed and 1,8-dihydroxy-3-methylanthracenedione (chrysophanol) was isolated and tested for antibacterial activities against four gram negative and five gram positive bacterial strains. The structures of chrysophanol was determined by chemical ...

  14. Assessment of Chronic Administration of Aloe Vera Gel On ...

    African Journals Online (AJOL)

    The study was designed to investigate the effects of chronic administration of Aloe vera gel extract on markers of hepatic damage, lipid profiles and erythrocyte osmotic fragility using the Wistar rats. Forty male Wistar rats divided into four groups of ten rats per group were used in the study. Group I which served as the control ...

  15. Comparative antimicrobial activities of aloe vera gel and leaf ...

    African Journals Online (AJOL)

    The comparative antimicrobial activities of the gel and leaf of Aloe vera were tested against Staphylococcus aureus, Pseudomonas aeruginosa, Trichophyton mentagraphytes, T. schoeleinii, Microsporium canis and Candida albicans. Ethanol was used for the extraction of the leaf after obtaining the gel from it. Antimicrobial ...

  16. Genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria arborescens.

    Science.gov (United States)

    Komatsu, Ken; Katayama, Yukie; Omatsu, Tsutomu; Mizutani, Tetsuya; Fukuhara, Toshiyuki; Kodama, Motoichiro; Arie, Tsutomu; Teraoka, Tohru; Moriyama, Hiromitsu

    2016-09-01

    The phytopathogenic fungus Alternaria spp. contains a variety of double-stranded RNA (dsRNA) elements of different sizes. Detailed analysis of next-generation sequencing data obtained using dsRNA purified from Alternaria arborescens, from which we had previously found Alternaria arborescens victorivirus 1, revealed the presence of another mycoviral-like dsRNA of approximately 2.5 kbp in length. When using the fungal mitochondrial genetic code, this dsRNA has a single open reading frame that potentially encodes an RNA-dependent RNA polymerase (RdRp) with significant to sequence similarity to those of viruses of the genus Mitovirus. Moreover, both the 5'- and 3'-untranslated regions have the potential to fold into stable stem-loop structures, which is characteristic of mitoviruses. Pairwise comparisons and phylogenetic analysis of the deduced amino acid sequences of RdRp indicated that the virus we identified in A. arborescens is a distinct member of the genus Mitovirus in the family Narnaviridae, designated as "Alternaria arborescens mitovirus 1" (AaMV1).

  17. Lipoma arborescens of the knee: a rare tumour in a black woman. A ...

    African Journals Online (AJOL)

    It weighed 520g and histology showed clusters of adipocytes with abundant pale cytoplasm as well as clusters of collagen fibres with elongated nuclei and bipolar cytoplasmic processes consistent with lipoma arborescens (LA). There is no recurrence at three years of regular follow-up. On the sheer size of this rare lesion, ...

  18. Biology of Pelosina arborescens PEARCEY, 1914, with comparative notes on Astrorhiza limicola SANDAHL, 1857 (Foraminifera: Astrorhizidae)

    DEFF Research Database (Denmark)

    Cedhagen, Tomas

    1993-01-01

    of the test are adaptations that also protect against bioturbation and predation but may restrict the respiratory surface. Field observations and laboratory experiments under anoxic conditions show that P arborescens can live anaerobically. Histological analysis of the protoplasm revealed large amounts...... of glycogen which may be used during anoxic periods. Some aspects of the biology are compared with Astrohiza limicola....

  19. Analyses of Aloe polysaccharides using carbohydrate microarray profiling

    DEFF Research Database (Denmark)

    Isager Ahl, Louise; Grace, Olwen M; Pedersen, Henriette Lodberg

    2018-01-01

    As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another and the effects they have on the human body are increasing. Polysaccharides present in the leaf mesophyll have been...... identified as the components responsible for the biological activities of Aloe vera, and they have been widely studied in the past decades. However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions as most of them require...... a complete or near-complete fractionation of the polymers. The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in Aloe leaf mesophyll. The method we chose is known as Comprehensive Microarray Polymer Profiling...

  20. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study).

    Science.gov (United States)

    Ehsani, Maryam; Amin Marashi, Mahmood; Zabihi, Ebrahim; Issazadeh, Maryam; Khafri, Soraya

    2013-01-01

    Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk diffusion, microdilution and direct contact test. In addition to the above bacterium, the Aloe vera gel effect on Staphylococcus aureus and Streptococcus mutans was evaluated. Disk diffusion test revealed that propolis ethanolic extracts (the alcohol concentration of 15 and 40%) and Aloe vera gel have antibacterial activities but aqueous extract of propolis did not show any effect in this test. The MICs for propolis ethanolic extracts, Aloe vera gel and aqueous extract of propolis (0% alcohol) were 313 µg/ml, 750 µg/ml, 2250 µg/ml, and ≥ 500 µg/ml respectively, much higher than the Chlorhexidine one. In direct contact test, contrary to Aloe vera, all three propolis extracts showed antibacterial effects on E. faecalis. The Aloe vera gel also showed significant antibacterial effect on S.aureus and S.mutans. The hydroalcoholic extracts of propolis and Aloe vera gel had antibacterial effects on E. faecalis, however, propolis is more potent than Aloe vera. The antibacterial effect of Aloe vera on S. aureus and S. mutans is low (MIC ≥ 2250 µg/ml). Appropriate concentrations of alcoholic extracts of propolis and some fractions of Aloe vera gel might be good choices for disinfecting the root canal in endodontic treatments.

  1. Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes.

    Science.gov (United States)

    Moriyama, Mariko; Moriyama, Hiroyuki; Uda, Junki; Kubo, Hirokazu; Nakajima, Yuka; Goto, Arisa; Akaki, Junji; Yoshida, Ikuyo; Matsuoka, Nobuya; Hayakawa, Takao

    2016-01-01

    Aloe has been used as a folk medicine because it has several important therapeutic properties. These include wound and burn healing, and Aloe is now used in a variety of commercially available topical medications for wound healing and skin care. However, its effects on epidermal keratinocytes remain largely unclear. Our data indicated that both Aloe vera gel (AVG) and Cape aloe extract (CAE) significantly improved wound healing in human primary epidermal keratinocytes (HPEKs) and a human skin equivalent model. In addition, flow cytometry analysis revealed that cell surface expressions of β1-, α6-, β4-integrin, and E-cadherin increased in HPEKs treated with AVG and CAE. These increases may contribute to cell migration and wound healing. Treatment with Aloe also resulted in significant changes in cell-cycle progression and in increases in cell number. Aloe increased gene expression of differentiation markers in HPEKs, suggesting roles for AVG and CAE in the improvement of keratinocyte function. Furthermore, human skin epidermal equivalents developed from HPEKs with medium containing Aloe were thicker than control equivalents, indicating the effectiveness of Aloe on enhancing epidermal development. Based on these results, both AVG and CAE have benefits in wound healing and in treatment of rough skin.

  2. Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

    Directory of Open Access Journals (Sweden)

    Samira Negahdari

    2017-01-01

    Conclusions: The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgf β 1 and Vegf-A genes in fibroblast cells treated with extracts.

  3. Aloe vera for treating acute and chronic wounds

    Directory of Open Access Journals (Sweden)

    Anthony D. Dat

    Full Text Available BACKGROUND: Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process.OBJECTIVE: To determine the effects of Aloe vera-derived products (for example dressings and topical gels on the healing of acute wounds (for example lacerations, surgical incisions and burns and chronic wounds (for example infected wounds, arterial and venous ulcers.METHODS:Search methods: We searched the Cochrane Wounds Group Specialised Register (9 September 2011, the Cochrane Central Register of Controlled Trials (CENTRAL (The Cochrane Library 2011, Issue 3, Ovid MEDLINE (2005 to August Week 5 2011, Ovid MEDLINE (In-Process & Other Non-Indexed Citations 8 September 2011, Ovid EMBASE (2007 to 2010 Week 35, Ovid AMED (1985 to September 2011 and EBSCO CINAHL (1982 to 9 September 2011. We did not apply date or language restrictions. Selection criteria: We included all randomised controlled trials that evaluated the effectiveness of Aloe vera, aloe-derived products and a combination of Aloe vera and other dressings as a treatment for acute or chronic wounds. There was no restriction in terms of source, date of publication or language. An objective measure of wound healing (either proportion of completely healed wounds or time to complete healing was the primary endpoint. Data collection and analysis: Two review authors independently carried out trial selection, data extraction and risk of bias assessment, checked by a third review author.MAIN RESULTS:Seven trials were eligible for inclusion, comprising a total of 347 participants. Five trials in people with acute wounds evaluated the effects of Aloe vera on burns, haemorrhoidectomy patients and skin biopsies. Aloe vera mucilage did not increase burn healing compared with silver sulfadiazine (risk ratio (RR 1.41, 95% confidence interval (CI 0.70 to 2.85. A reduction in

  4. Aloe vera for treating acute and chronic wounds.

    Science.gov (United States)

    Dat, Anthony D; Poon, Flora; Pham, Kim B T; Doust, Jenny

    2012-02-15

    Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process. To determine the effects of Aloe vera-derived products (for example dressings and topical gels) on the healing of acute wounds (for example lacerations, surgical incisions and burns) and chronic wounds (for example infected wounds, arterial and venous ulcers). We searched the Cochrane Wounds Group Specialised Register (9 September 2011), the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2011, Issue 3), Ovid MEDLINE (2005 to August Week 5 2011), Ovid MEDLINE (In-Process & Other Non-Indexed Citations 8 September 2011), Ovid EMBASE (2007 to 2010 Week 35), Ovid AMED (1985 to September 2011) and EBSCO CINAHL (1982 to 9 September 2011). We did not apply date or language restrictions. We included all randomised controlled trials that evaluated the effectiveness of Aloe vera, aloe-derived products and a combination of Aloe vera and other dressings as a treatment for acute or chronic wounds. There was no restriction in terms of source, date of publication or language. An objective measure of wound healing (either proportion of completely healed wounds or time to complete healing) was the primary endpoint. Two review authors independently carried out trial selection, data extraction and risk of bias assessment, checked by a third review author. Seven trials were eligible for inclusion, comprising a total of 347 participants. Five trials in people with acute wounds evaluated the effects of Aloe vera on burns, haemorrhoidectomy patients and skin biopsies. Aloe vera mucilage did not increase burn healing compared with silver sulfadiazine (risk ratio (RR) 1.41, 95% confidence interval (CI) 0.70 to 2.85). A reduction in healing time with Aloe vera was noted after haemorrhoidectomy (RR 16.33 days, 95% CI 3.46 to 77.15) and there was

  5. Antibacterial activity of aloe emodin and aloin A isolated from Aloe ...

    African Journals Online (AJOL)

    GRACE

    2006-06-02

    Jun 2, 2006 ... A. excelsa also referred to as the Nobel Aloe is widespread in Africa with great abundance in Zimbabwe. Aloe emodin and aloin A are known to occur in both commercial viable species of aloes (Aloe vera and A. ferox), but has not previously been isolated from A. excelsa. Although extensive research had ...

  6. Cytogenetic toxicity of Aloe vera (a medicinal plant).

    Science.gov (United States)

    Verma, Anjana; Gupta, Ashok K; Kumar, Amod; Khan, Parimal K

    2012-01-01

    The cytogenetic toxicity of the crude leaf extract of Aloe vera, a medicinal plant, was evaluated in two test systems, onion and Swiss albino mice, using their root tip meristematic and bone marrow cells, respectively. No significant increase in structural abnormalities in chromosomes was observed, but a marked increase in cells with chromosome-number anomalies was found. The extract, however, significantly increased the mitotic index of both cell types.

  7. Effect of Aloe Barbadensis on Rat's Uterine Contractility | Iranloye ...

    African Journals Online (AJOL)

    The present study was undertaken to investigate the effect of the aqueous extract of Aloe barbadensis on the contractility of the uterine stip of a rat. Aqueous extract at final bath concentrations (FBC)1x10-4 mg/ml to 3x101 mg/ml produced a progressive increase in frequency of contraction of the uterine strip. The force of ...

  8. Immunohistochemical studies on the effect of Aloe vera on the ...

    African Journals Online (AJOL)

    resulted in 30 and 34% decreases in blood sugar levels of n0-STZ-diabetic rats, after 2 and 3 hr of administration of the extract respectively (Okyar et al. 2001a), and 11 and 14% reductions in blood glucose levels 3 and 4 hr after administration of Aloe leaf-gel extract. However these effects could not be repeated with the ...

  9. The increasing of fibroblast growth factor 2, osteocalcin, and osteoblast due to the induction of the combination of Aloe vera and 2% xenograft concelous bovine

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2012-12-01

    Full Text Available Background: To make a successfull denture prominent ridge is needed, preservation on tooth extraction socket is needed in order to prevent alveol bone resorption caused by revocation trauma. An innovative modification of the material empirically suspected to be able reduce inflammation caused by the revocation trauma is a combination of Aloe vera and xenograft concelous bovine (XCB and Aloe vera is a biogenic stimulator and accelerating the growth of alveolar ridge bone after tooth extraction. Purpose: The research was aimed to determine of the increasing alveol bone formation by inducing the combination of Aloe vera and 2% xenograft concelous bovine. Methods: To address the problems, the combination of Aloe vera and xenograft concelous bovine was induced into the tooth extraction sockets of Cavia cabayas which devided on 8 groups. Groups control, filled with XCB, Aloe vera and Aloe vera and XCB combination, at 7 days and 30 days after extraction. Afterwards, immunohistochemical examination was conducted to examine the expressions of FGF-2 and osteocalcin, as the product of the growth of osteoblasts. Results: There were significantly increases expression of FGF-2 and osteocalcyn on group which filled with XCB, Aloe vera and combined Aloe vera and XCB. Conclusion: It may be concluded that the induction of the combination of Aloe vera and xenograft concelous bovine into the tooth sockets can enhance the growth expressions of FGF-2 and osteocalcin as the product of osteoblasts, thus, the growth of alveolar bone was increased.Latar belakang: Untuk keberhasilan pembuatan gigitiruan diperlukan ridge yang prominent, maka diperlukan suatu preservasi soket pencabutan gigi untuk mencegah terjadinya resopsi tulang alveolar akibat trauma pencabutan. Suatu inovasi modifikasi bahan yang diduga secara empiris dapat mengurangi keradangan karena trauma pencabutan adalah berupa kombinasi Aloe vera dan xenograft concelous bovine (XCB. Aloe vera yang merupakan

  10. Fungitoxic activity of extracts of some medicinal plants on Pythium ...

    African Journals Online (AJOL)

    Abstract. The in vitro fungitoxic activity of crude extracts of ginger (Zingiber officinale), bitter-kola (Garcinia cola), aloe (Aloe vera) and neem (Azadirachta indica) was tested on Pythium ... The results showed that mean percentage inhibition of mycelia growth was highest in plates containing ginger extract; followed by aloe.

  11. Genome size variation in the Artemisia arborescens complex (Asteraceae, Anthemideae) and its cultivars.

    Science.gov (United States)

    Garcia, Sònia; Garnatje, Teresa; Twibell, John D; Vallès, Joan

    2006-03-01

    Different wild Mediterranean populations of Artemisia arborescens from diverse locations representing its geographical distribution, as well as some of its well-known cultivars and some specimens cultivated as ornamentals in gardens, streets, roads and nurseries, were analysed for genome size. Other closely related species endemic to Macaronesia, Artemisia canariensis, Artemisia argentea, and Artemisia gorgonum, were also analysed, and their nuclear DNA amount has been related to the biogeography of this group of species. Additionally, 5 populations of the closely related Artemisia absinthium were analysed to establish comparisons. Measurements acquired by flow cytometry ranged from 8.29 to 11.61 pg for 2C values. Statistically significant differences of 2C nuclear DNA amounts with respect to factors such as insularity or domestication have been detected. However, quite a low intraspecific genome size variation has been found in these species. Furthermore, the study also addressed the possible hybrid origins and possible misidentifications of some of the supposed cultivars of A. arborescens.

  12. Aloe in Angola (Asphodelaceae: Alooideae

    Directory of Open Access Journals (Sweden)

    R. R. Klopper

    2009-08-01

    Full Text Available Botanical exploration of Angola was virtually impossible during the almost three-decade-long civil war. With more areas becoming accessible, there is, however, a revived interest in the flora of this country. A total of 27 members of the genus Aloe L. have been recorded for Angola. It is not unlikely that new taxa will be discovered, and that the distribution ranges of others will be expanded now that botanical exploration in Angola has resumed. This manuscript provides a complete taxonomic treatment of the known Aloe taxa in Angola. It includes, amongst other information, identification keys, descriptions and distribution maps.

  13. Aloe in Angola (Asphodelaceae: Alooideae

    Directory of Open Access Journals (Sweden)

    R. R. Klopper

    2009-12-01

    Full Text Available Botanical exploration of Angola was virtually impossible during the almost three-decade-long civil war. With more areas becoming accessible, there is, however, a revived interest in the flora of this country. A total of 27 members of the genus Aloe L. have been recorded for Angola. It is not unlikely that new taxa will be discovered, and that the distribution ranges of others will be expanded now that botanical exploration in Angola has resumed. This manuscript provides a complete taxonomic treatment of the known Aloe taxa in Angola. It includes, amongst other information, identification keys, descriptions and distribution maps.

  14. Lipoma Arborescens: Review of an Uncommon Cause for Swelling of the Knee

    Directory of Open Access Journals (Sweden)

    M. De Vleeschhouwer

    2016-01-01

    Full Text Available Lipoma arborescens is a rare cause of chronic monoarticular arthritis, with only a few cases reported in the literature. It is most commonly seen in the knee, but cases in other joints such as the wrist, shoulder, and elbow have also been described. It is a benign condition, in which the subsynovial tissue is replaced diffusely by mature fat cells. We describe a case involving the knee and discuss the symptoms, diagnosis, and treatment.

  15. Genome sequence of a novel victorivirus identified in the phytopathogenic fungus Alternaria arborescens.

    Science.gov (United States)

    Komatsu, Ken; Katayama, Yukie; Omatsu, Tsutomu; Mizutani, Tetsuya; Fukuhara, Toshiyuki; Kodama, Motoichiro; Arie, Tsutomu; Teraoka, Tohru; Moriyama, Hiromitsu

    2016-06-01

    Strains of the phytopathogenic fungus Alternaria spp. have been found to contain a variety of double-stranded RNA (dsRNA) elements indicative of mycovirus infection. Here, we report the molecular characterization of a novel dsRNA mycovirus, Alternaria arborescens victorivirus 1 (AaVV1), from A. arborescens, the tomato pathotype of A. alternata. Using next-generation sequencing of dsRNA purified from an A. arborescens strain from the United States of America, we found that the AaVV1 genome is 5203 bp in length and contains two open reading frames (ORF1 and 2) that overlap at the tetranucleotide AUGA. Proteins encoded by ORF1 and ORF2 showed significant similarities to the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp), respectively, of dsRNA mycoviruses of the genus Victorivirus. Pairwise comparisons and phylogenetic analysis of the deduced amino acid sequences of both CP and RdRp indicated that AaVV1 is a member of a distinct species of the genus Victorivirus in the family Totiviridae.

  16. Flowering of siberian peashrub (Caragana arborescens Lam. and its forage value to pollinating insects

    Directory of Open Access Journals (Sweden)

    Ernest Stawiarz

    2014-09-01

    Full Text Available Under the conditions of Lublin (central-eastern Poland, observations were conducted on the flowering of Caragana arborescens shrubs for two growth seasons as well as nectar and pollen production by the flowers of this species was investigated. The flowering period of Caragana arborescens began at the turn of April and May and lasted for 24 days, while for a single flower it was 6.30 ± 0.79 days. The average weight of nectar, sugars and pollen produced by 10 Siberian peashrub flowers was, respectively: 46.70 ± 8.39 mg, 22.79 ± 6.10 mg, and 4.45 ± 0.34 mg. The nectar sugar concentration amounted 49.60 ± 10.08%. Pollen grains reached average dimensions of 21.94 ± 0.76 µm x 21.52 ± 0.71 µm. During a growing season, one Caragana arborescens shrub can provide on average 101.8 g of nectar, 49.7 g of sugars, and 9.7 g of pollen.

  17. Aloe-Vera: A Nature's Gift to Children.

    Science.gov (United States)

    Gupta, Neha; Bhat, Manohar; Devi, Prabha; Girish

    2010-01-01

    (1)Aloe-Barbadensis Mill (Liliaceae) is used in the traditional medicine of Mexico and other countries for anti-inflammatory and cosmetic purposes (Diez-Martinez 1981, Grindlay and Reynolds 1986). Two components are obtained from the fresh leaves of Aloe-Barbadensis, a bitter yellow juice (exudate), which drains from the transversally cut leaves used as a laxative (Ishii et al 1990) and a mucilaginous gel from leaf parenchyma, which has been used as a remedy for a variety of pathological states such as arthritis, gout, acne, dermatitis, burns and peptic ulcers induced by epithelial alterations (Cap-passo and Ganginella 1997, Reynolds and Dweek 1999). The aim of this study is to evaluate efficacy of Aloe-Vera gel as a healing agent in an endodontic procedure called pulpotomy. Fifteen primary molars were treated for pulpotomy using 'Aloe-Vera gel'. Patients were recalled after 1 month to check for any clinical symptoms. None of the patients reported with clinical symptoms of pain, mobility, abscess and histopathological evaluation done following extraction after 2 months showed positive signs of healing.

  18. Photocarcinogenesis study of aloe vera [CAS NO. 481-72-1(Aloe-emodin)] in SKH-1 mice (simulated solar light and topical application study).

    Science.gov (United States)

    2010-09-01

    The popular recognition of the Aloe barbadensis Miller (Aloe vera) plant as a therapeutic dermatologic agent has led to the widespread incorporation of Aloe vera leaf extracts in skincare products. Studies have suggested that Aloe vera in skincare preparations may enhance the induction of skin cancer by ultraviolet radiation. A 1-year study was conducted in mice to determine whether the topical application of creams containing Aloe vera plant extracts (aloe gel, whole leaf, or decolorized whole leaf) or creams containing aloe-emodin would enhance the photocarcinogenicity of simulated solar light (SSL). 1-YEAR STUDY: groups of 36 male and 36 female Crl:SKH-1 (hr -/hr -) hairless mice received topical applications of control cream or creams containing 3% or 6% (w/w) aloe gel, whole leaf, or decolorized whole leaf or 7.46 or 74.6 µg/g aloe-emodin to the dorsal skin region each weekday morning. The mice were irradiated with SSL emitted from filtered 6 kW xenon arc lamps each weekday afternoon. The topical applications of creams and irradiance exposures were conducted 5 days per week for a period of 40 weeks. A 12-week recovery/observation period followed the 40-week treatment/exposure period. Additional groups of 36 male and 36 female mice received no cream and were exposed to 0.00, 6.85, 13.70, or 20.55 mJ⋅CIE/cm2 SSL per day. Mice that received no cream treatment and were exposed to increasing levels of SSL showed significant SSL exposure-dependent decreases in survival and significant increases in the in-life observations of skin lesion onset, incidence, and multiplicity, and significant SSL exposure-dependent increases in the incidences and multiplicities of histopathology-determined squamous cell nonneoplastic skin lesions (squamous hyperplasia and focal atypical hyperplasia) and squamous cell neoplasms (papilloma, carcinoma in situ, and/or carcinoma). Squamous cell neoplasms were not detected in mice that received no SSL exposure. The topical treatment with

  19. Determination of aloesin and aloeresin A for the detection of aloe in beverages.

    Science.gov (United States)

    Dell'Agli, Mario; Giavarini, Flavio; Ferraboschi, Patrizia; Galli, Giovanni; Bosisio, Enrica

    2007-05-02

    This work describes a sensitive high-performance liquid chromatography (HPLC) method for the quantification of aloesin and aloeresin A in alcoholic beverages containing aloe as a flavoring agent. The compounds were prepared from Aloe ferox juice. Sephadex LH20 and ion-exchange resin AG1X2 column chromatography were used for aloesin. Aloeresin A was obtained by Sephadex LH20 and silica gel column chromatography followed by purification on Discovery DSC-18 solid-phase extraction tubes. A 98 mg amount of aloesin (>99% purity) and 34 mg of aloeresin A (>98% purity) were recovered from 2.5 g of aloe juice. The HPLC method was validated, and intra- and interday performances were established. In-house validation was carried out by analyzing samples of beverages with and without aloe as a flavoring agent.

  20. Aloe vera for prevention and treatment of infusion phlebitis.

    Science.gov (United States)

    Zheng, Guo Hua; Yang, Liu; Chen, Hai Ying; Chu, Jian Feng; Mei, Lijuan

    2014-06-04

    Up to 80% of hospitalised patients receive intravenous therapy at some point during their admission. About 20% to 70% of patients receiving intravenous therapy develop phlebitis. Infusion phlebitis has become one of the most common complications in patients with intravenous therapy. However, the effects of routine treatments such as external application of 75% alcohol or 50% to 75% magnesium sulphate (MgSO4) are unsatisfactory. Therefore, there is an urgent need to develop new methods to prevent and alleviate infusion phlebitis. To systematically assess the effects of external application of Aloe vera for the prevention and treatment of infusion phlebitis associated with the presence of an intravenous access device. The Cochrane Peripheral Vascular Diseases Group Trials Search Co-ordinator (TSC) searched the Specialised Register (last searched February 2014) and CENTRAL (2014, Issue 1). In addition the TSC searched MEDLINE to week 5 January 2014, EMBASE to Week 6 2014 and AMED to February 2014. The authors searched the following Chinese databases until 28 February 2014: Chinese BioMedical Database; Traditional Chinese Medical Database System; China National Knowledge Infrastructure; Chinese VIP information; Chinese Medical Current Contents; Chinese Academic Conference Papers Database and Chinese Dissertation Database; and China Medical Academic Conference. Bibliographies of retrieved and relevant publications were searched. There were no restrictions on the basis of date or language of publication. Randomised controlled trials (RCTs) and quasi-randomised controlled trials (qRCTs) were included if they involved participants receiving topical Aloe vera or Aloe vera-derived products at the site of punctured skin, with or without routine treatment at the same site. Two review authors independently extracted the data on the study characteristics, description of methodology and outcomes of the eligible trials, and assessed study quality. Data were analysed using RevMan 5

  1. A comparative study of baby immature and adult shoots of Aloe vera on UVB-induced skin photoaging in vitro.

    Science.gov (United States)

    Hwang, Eunson; Kim, Su Hyeon; Lee, Sarah; Lee, Choong Hwan; Do, Seon-Gil; Kim, Jinwan; Kim, Sun Yeou

    2013-12-01

    Ultraviolet (UV) irradiation induces photo-damage of the skin, which in turn causes depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase (MMP) expression. The production of type I procollagen is regulated by transforming growth factor-β1 (TGF-β1) expression; the activation of MMP is also correlated with an increase of interleukin-6 (IL-6). Aloe barbadensis M. (Aloe vera) is widely used in cosmetic and pharmaceutical products. In this study, we examined whether baby aloe shoot extract (BAE, immature aloe extract), which is from the one-month-old shoots of Aloe vera, and adult aloe shoot extract (AE), which is from the four-month-old shoots of Aloe vera, have a protective effect on UVB-induced skin photoaging in normal human dermal fibroblasts (NHDFs). The effects of BAE and AE on UVB-induced photoaging were tested by measuring the levels of reactive oxygen species, MMP-1, MMP-3, IL-6, type I procollagen, and TGF-β1 after UVB irradiation. We found that NHDF cells treated with BAE after UVB-irradiation suppressed MMP-1, MMP-3, and IL-6 levels compared to the AE-treated cells. Furthermore, BAE treatment elevated type I procollagen and TGF-β1 levels. Our results suggest that BAE may potentially protect the skin from UVB-induced damage more than AE. Copyright © 2013 John Wiley & Sons, Ltd.

  2. Aloe Vera Effervescent Tablet Formulation: Comparation Study of Acid-Alkali-Aloe Powder and Maltodextrin Concentration

    OpenAIRE

    Kasmawarni, Kasmawarni; Utomo, Pramono Putro

    2010-01-01

    The research on The Application of Effervescent Technology of Aloe vera as Functional Drink has been done. The objectives were to obtain effrvescent tablet prefered by the panelist as well as nutritious as aloe vera-based functional food also to obtain appropriate formula resulting good quality of effervescent tablet. Formulation of effervescent tablet with treatments of maltodextrin concentration were done. The result showed that the best formulas of aloe vera effervescent tablet were aloe v...

  3. Benefits of Aloe vera in dentistry

    Science.gov (United States)

    Mangaiyarkarasi, S. P.; Manigandan, T.; Elumalai, M.; Cholan, Priyanka K.; Kaur, Roopam Pal

    2015-01-01

    Aloe vera (Aloe barbadensis) is a plant that belongs to Liliaceae family. The name Aloe derives from the Arabic word “Alloeh” meaning shining bitter substance while “vera” in Latin means true. It contains various minerals and vitamins. It has got various properties such as immunomodulatory, antiviral and antiinflammatory in nature. A. vera can play a significant role in dentistry in treatment of lichen planus, oral submucous fibrosis, recurrent aphthous stomatitis, alveolar osteitis, periodontitis, etc. PMID:26015726

  4. Antiviral activity of Aloe vera against herpes simplex virus type 2: An ...

    African Journals Online (AJOL)

    In this study we tested the antiviral activity of a crude hot glycerine extract of Aloe vera gel which was grown in Bushehr (Southwest of Iran) against HSV-2 replication in Vero cell line. The extract showed antiviral activity against HSV-2 not only before attachment and entry of virus to the Vero cells but also on post attachment ...

  5. The protective effect of aloe vera juice on lindane induced hepatotoxicity and genotoxicity.

    Science.gov (United States)

    Etim, O E; Farombi, E O; Usoh, I F; Akpan, E J

    2006-10-01

    The protective effect of fresh aloe vera (AV) leaves extract on lindane (LD) - induced hepatoxicity and genotoxicity was studied. Serum levels of hepatic enzyme markers: glutamate pyruvate transaminase (GPT), glutamate oxaloacetate transaminase (GOT), gamma glutamyl transferase (GGT) and alkaline phosphatase (ALP) were determined after oral administration of aloe vera leaves extract and lindane. The level of polychromatic erythrocytes was also observed. Pretreatment with aloe vera leaves extract at concentration of 1.0 ml/kg body weight significantly decreased (P<0.05) the serum levels of GPT (by 41.8%), GOT (by 36.5%), GGT (by 14.3%) and ALP (by 10.7%) induced by 100mg/kg body weight of lindane. The level of polychromatic erythrocytes observed was not statistically significant when compared to control.

  6. Stimulation of osteoblast activity by induction of Aloe vera and xenograft combination

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2011-12-01

    Full Text Available Background: Tooth extraction is generally followed by alveolar ridge resorption that later can cause flat ridge. Aloe vera have biogenic stimulator and hormone activities for wound healing. Purpose: This study was aimed to know osteoblast activities in alveolar bone after induction of Aloe vera and XCB combination. Methods: Fifty four of Cavia cabaya were divided into three main groups. Group I was control group. Group II was filled with xenograft concelous bovine (XCB and group III was filled with the combination of Aloe vera gel and XCB. Then, each group was divided into three sub groups according to timing, they are 14, 30, and 60 days after tooth extraction and application. Histology and morphology examination were performed on the harvested specimens. Results: There were significant differences between the control group and the other groups filled with the combination of Aloe vera and XCB. Conclusion: In conclusion, the application of Aloe vera gel and xenograft combination decrease the number of osteoclast and increase the number of osteoblast in post tooth extraction alveolar bone structure indicating the new growth of alveolar bone.Latar belakang: Pencabutan gigi pada umumnya selalu diikuti resopsi tulang alveolar, sehingga bila terjadi dalam waktu yang lama ridge akan menjadi flat. Aloe vera adalah bahan stimulasi biogenik dan mempunyai aktivitas hormon untuk proses penyembuhan luka. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui aktivitas osteoblas pada tulang alveol dengan pemberian kombinasi Aloe vera gel dan xenograft concelous bovine (XCB. Metode: Lima puluh empat ekor Cavia cabaya, dibagi menjadi 3 kelompok besar, kelompok pertama adalah kelompok kontrol yaitu hanya dilakukan pencabutan saja tanpa perlakuan, kelompok ke-2 yaitu kelompok yang setelah dicabut diberi XCB saja dan kelompok ke-3 yaitu kelompok yang setelah pencabutan diberi kombinasi Aloe vera gel dengan XCB pada luka bekas pencabutan gigi. Kemudian masing

  7. Cytotoxic derivatives of withanolides isolated from the leaves of Acnistus arborescens; Derivados citotoxicos de vitanolidos isolados das folhas de Acnistus arborescens

    Energy Technology Data Exchange (ETDEWEB)

    Minguzzi, Sandro, E-mail: sming@uems.b [Universidade Estadual de Mato Grosso do Sul, Navirai, MS (Brazil). Curso de Quimica; Barata, Lauro E.S. [Universidade Estadual de Campinas (IQ/UNICAMP), SP (Brazil). Inst. de Quimica; Cordell, Geoffrey A. [Natural Products Inc., Evanston, IL (United States)

    2011-07-01

    In view of anticancer activity of 7 beta-acetoxywithanolide D (2) and 7beta-16beta-diacetoxywithonide D (3), isolated from the leaves of Acnistus arborescens (Solanaceae), five withanolide derivatives were obtained and their structures were determined by NMR, MS and IV data analysis. The in vitro anticancer activity of these derivatives was evaluated in a panel of cancer cell lines: human breast (BC-1), human lung (Lu1), human colon (Col2) and human oral epidermoid carcinoma (KB). Compounds 2a (acetylation of 2), 3b (oxidation of 3) and 2c (hydrogenation of 2) exhibited the highest anticancer activity against human lung cancer cells, with ED{sub 50} values of 0.19, 0.25 and 0.63 mug/mL, respectively. (author)

  8. Lipoma arborescens associated with osseous/chondroid differentiation in subdeltoid bursa.

    Science.gov (United States)

    Kim, Ryuh Sup; Kim, Young Tae; Choi, Jong Min; Shin, Sang Hyun; Kim, Yeo Ju; Kim, Lucia

    2013-07-01

    Lipoma arborescens (LA) is a rare benign lesion of unknown etiology. It is characterized histologically by villous proliferation of the synovial membrane and diffuse replacement of the subsynovial tissue by mature fat cells. This condition affects the knee joint most commonly. Cases involving other locations including glenohumeral joint,[1] hip,[2] elbow,[3] hand[4] and ankle[5] have been rarely described. Involvement of the subdeltoid bursa has also been reported, but to date no case has described LA with osseous/chondroid differentiation of this bursa. Another significant finding in our case was the coexistence of LA with intermuscular lipoma, SLAP lesion and labral cyst.

  9. Daya Hambat Ekstrak Aloe Vera terhadap pertumbuhan Staphylococcus Aureus

    OpenAIRE

    Rahmat, drg.Sp,Pros

    2011-01-01

    Dari hasil penelitian , maka dapat disimpulkan bahwa ekstrak Aloe Vera dapat menghambat pertumbuhan bakteri Stafhylococcus aureus, dan kadar hambat minimal ekstrak Aloe Vera adalah pada konsentrasi 25%. Tujuan Penelitan Ini adalah untuk mengetahui efektifitas ekstrak Aloe vera dalam menghambat pertumbuhan bakteri Stafhylococcus aureus dan daya hambat menimal, (DHM) terhadap pertumbuhan bakteri tersebut. Metode yang digunakan adalah pertumbuhan ekstrak Aloe vera, penegnceran ekstrak , pemur...

  10. EXAFS analysis of cations distribution in structure of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} nanoparticles obtained by hydrothermal method in aloe vera extract solution

    Energy Technology Data Exchange (ETDEWEB)

    Wongpratat, Unchista [Materials Science and Nanotechnology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Maensiri, Santi [School of Physics, Institute of Science, Suranaree University, Nakhonratchasima 30000 (Thailand); Swatsitang, Ekaphan, E-mail: ekaphan@kku.ac.th [Materials Science and Nanotechnology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Integrated Nanotechnology Research Center, Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand (Thailand)

    2016-09-01

    Graphical abstract: It is obvious from the M–H curves at room temperature of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles that partially substitution of the lower Bohr magneton (2 μ{sub B}) and smaller atomic radii (0.55 Å at A site, 0.69 Å at B site) of Ni{sup 2+} ions on the higher Bohr magneton (3 μ{sub B}) and larger atomic radii (0.58 Å at A site, 0.74 Å at B site) of Co{sup 2+} ions can increase the saturation magnetization (M{sub s}) of sample with x = 0.75 to approximately 1.4 times of sample with x = 0, due to the increase of the aspect ratio (surface to volume) of nanoparticles, as a result of particle size decreasing from 37.03 to 12.63 nm. In addition to this, the ferrimagnetic behavior of CoFe{sub 2}O{sub 4} has been changed to superparamagnetic behavior with the dramatic decrease of the coercivity from 1365.60 to 63.15 Oe. - Highlights: • Magnetic behavior of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} NPs depends on Ni content and size of NPs. • Distribution of Co{sup 2+} and Ni{sup 2+} ions in the structure results in the increase of M{sub s}. • Superparamagnetic behavior is observed with increasing of the aspect ratio. • M{sub s} is increased by a factor 1.4 to a value of 57.57 emu/g in Co{sub 0.25}Ni{sub 0.75}Fe{sub 2}O{sub 4}. • H{sub c} is decreased by a factor 20 to a value of 63.15 Oe in Co{sub 0.25}Ni{sub 0.75}Fe{sub 2}O{sub 4}. - Abstract: Effect of cations distribution upon EXAFS analysis on magnetic properties of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles prepared by the hydrothermal method in aloe vera extract solution were studied. XRD analysis confirmed a pure phase of cubic spinel ferrite of all samples. Changes in lattice parameter and particle size depended on the Ni content with partial substitution and site distributions of Co{sup 2+}, Ni{sup 2+} ions of different ionic radii at both tetrahedral and octahedral sites in the

  11. α-Mangostin from Cratoxylum arborescens (Vahl Blume Demonstrates Anti-Ulcerogenic Property: A Mechanistic Study

    Directory of Open Access Journals (Sweden)

    Heyam M. A. Sidahmed

    2013-01-01

    Full Text Available Cratoxylum arborescens (Vahl Blume is an Asian herbal medicine with versatile ethnobiological properties including treatment of gastric ulcer. This study evaluated the antiulcerogenic mechanism(s of α-mangostin (AM in a rat model of ulcer. AM is a prenylated xanthone derived through biologically guided fractionation of C. arborescens. Rats were orally pretreated with AM and subsequently exposed to acute gastric lesions induced by ethanol. Following treatment, ulcer index, gastric juice acidity, mucus content, histological and immunohistochemical analyses, glutathione (GSH, malondialdehyde (MDA, nitric oxide (NO, and nonprotein sulfhydryl groups (NP-SH were evaluated. The anti-Helicobacter pylori, cyclooxygenase-2 (COX-2 inhibitory effect, and antioxidant activity of AM were also investigated in vitro. AM (10 and 30 mg/kg inhibited significantly (P<0.05 ethanol-induced gastric lesions by 66.04% and 74.39 %, respectively. The compound induces the expression of Hsp70, restores GSH levels, decreases lipid peroxidation, and inhibits COX-2 activity. The minimum inhibitory concentration (MIC of AM showed an effective in vitro anti-H. pylori activity. The efficacy of the AM was accomplished safely without presenting any toxicological parameters. The results of the present study indicate that the antioxidant properties and the potent anti-H. pylori, in addition to activation of Hsp70 protein, may contribute to the gastroprotective activity of α-mangostin.

  12. Composition and antimicrobial activity of essential oils of Artemisia judaica, A. herba-alba and A. arborescens from Libya

    Directory of Open Access Journals (Sweden)

    Janaćković Peđa

    2015-01-01

    Full Text Available The essential oils obtained by hydrodistillation from the aerial parts of Artemisia judaica L., Artemisia herba-alba Asso. and Artemisia arborescens L. (cultivated from Libya, were analyzed by GC and GC-MS. The antimicrobial properties were determined using the broth microdilution method against eight bacterial species: Bacillus cereus (clinical isolate, Micrococcus flavus (ATCC10240, Listeria monocytogenes (NCTC7973, Staphylococcus aureus (ATCC6538, Escherichia coli (ATCC35210, Pseudomonas aeruginosa (ATCC27853, Salmonella typhimurium (ATCC13311, Enterobacter cloacae (human isolates and eight fungal species: Aspergillus niger (ATCC6275, A. ochraceus (ATCC12066, A. versicolor (ATCC11730, A. fumigatus (ATCC1022, Penicillium ochrochloron (ATCC9112, P. funiculosum (ATCC10509, Trichoderma viride (IAM5061 and Candida albicans (human isolate. The major constituents of A. arborescens oil were sesquiterpene hydrocarbons (47.4%. Oxygenated monoterpenes were the dominant constituents in the A. judaica and A. herba-alba oils (54.2% and 77.3%, respectively. Camphor (24.7% and chamazulene (20.9% were the major components in the essential oil of A. arborescens, chrysanthenone (20.8%, cis-chrysanthenyl acetate (17.6% and cis-thujone (13.6% dominated in the A. herba-alba oil, and the major constituents in the A. judaica oil were piperitone (30.21% and cis-chrysanthenol (9.1%. The best antimicrobial activity was obtained for A. judaica oil and the lowest effect was noticed in A. arborescens oil. The effect of the tested oils was higher against Gram (+ than Gram (- bacteria. All three oils showed the best antibacterial activity against Listeria monocytogenes and the lowest against Pseudomonas aeruginosa, Escherichia coli, Enterobacter cloacae, compared to streptomycin and ampicillin. All three oils showed better antifungal activities than ketoconazole, except A. arborescens oil against Aspergillus niger. [Projekat Ministarstv nauke Republike Srbije, br. 173029

  13. Effects of Six Weeks Endurance Training and Aloe Vera Supplementation on COX-2 and VEGF Levels in Mice with Breast Cancer

    Science.gov (United States)

    Shirali, Saeed; Barari, Alireza; Hosseini, Seyed Ahmad; Khodadi, Elaheh

    2017-01-01

    The aim of this study was to determine effects of six weeks endurance training and Aloe Vera supplementation on COX-2 and VEGF levels in mice with breast cancer. For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Aloe Vera and Aloe Vera + training. Breast cancer tumors were generated in mice by implantind. The training program comprised six weeks of swimming training accomplished in three sessions per week. Training time started with 10 minutes on the first day and increased to 60 minutes in the second week and the water flow rate was increased from 7 to 15 liters per minute at a constant rate. Aloe Vera extract at a dose of 300 mg/kg BW was administrated to rats by intraperitoneal injection. At the end of the study period, rats were anesthetized and blood samples were taken. Significant differences were concluded at pAloe Vera extract caused significant decrease in the COX-2 level in the cancer group. Also, in the training (swimming exercise) and Aloe Vera + training cancer groups, we observed significant decrease in the VEGF level as compared to controls. Our results suggest that Aloe Vera and training inhibit the COX pathway and cause decrease production of prostaglandin E2. Hence administration of Aloe Vera in combination with endurance training might synergistically improve the host milieu in mice bearing breast cancers. Creative Commons Attribution License

  14. Possible interaction between sevoflurane and Aloe vera.

    Science.gov (United States)

    Lee, Anna; Chui, Po Tong; Aun, Cindy S T; Gin, Tony; Lau, Angel S C

    2004-10-01

    To describe a patient with massive intraoperative bleeding after oral consumption of Aloe vera tablets. A 35-year-old woman lost 5 L of blood during surgery as a result of a possible herb-drug interaction between Aloe vera and sevoflurane. Aloe vera is a common herb used for antiinflammatory and antiarthritic activity, as well as antibacterial, hypoglycemic, and lipid-lowering effects. Compounds contained within Aloe vera can cause a reduction in prostaglandin synthesis, which may inhibit secondary aggregation of platelets. Sevoflurane inhibits thromboxane A(2) formation by suppression of cyclooxygenase activity, impairs platelet aggregation, and prolongs bleeding. Although the vascularity and size of the hemangioma were the most important factors for the massive intraoperative blood loss, concomitant use of sevoflurane and Aloe vera played a contributory role. An objective causality assessment revealed that this adverse event was possible as a result of the sevoflurane and Aloe vera interaction. There is a potential herb-drug interaction between Aloe vera and sevoflurane based on the antiplatelet effects of these 2 agents. Herbal medications with antiplatelet potential should be discontinued before anesthesia and surgery.

  15. Evaluation of the anti-ulcer and toxicity profile of Aloe buettneri in ...

    African Journals Online (AJOL)

    The anti-ulcerogenic potential of the leaf methanol extract of Aloe buettneri A. Berger was investigated using three methods of gastric lesion induction in experimental Wistar rats (150-200 g) and mice (20-25 g): 1. HCl/ethanol-induced gastic lesions, 2. Indomethacin-HCl/ethanol-induced gastric lesions, and 3, Pylorus ...

  16. Efficacy of early chick nutrition with Aloe vera and Azadirachta indica on gut health and histomorphometry in chicks.

    Science.gov (United States)

    Sujatha, Tamilvanan; Abhinaya, Sivasankar; Sunder, Jai; Thangapandian, Marudhai; Kundu, Anandamoy

    2017-06-01

    This study was conducted with an aim of studying the efficacy of water supplements of Aloe vera and Azadirachta indica (neem) during pre-starter age (0-2 weeks) on gut health and histomorphometry in Vanaraja chicks. A total of 192 day old Vanaraja chicks were randomly assigned to one of four herbal water treatments throughout the experimental pre-starter stage (0-2 weeks) in a completely randomized design. Each treatment was given four replicates consisting of 12 chicks per replicate. Water treatments comprised T1: Control with regular antibiotic supplement, T2: 3 ml Aloe juice per chick per day, T3: 3 ml neem extract per chick per day, T4: 1.5 ml Aloe and 1.5 ml neem per chick per day. Gut culture was done for Escherichia coli and Lactobacillus sps. and gut histomorphometry in 24 gut samples at 14 days of age. This study revealed that supplementation of A. vera and neem in water significantly (pAloe supplementation. Villus height: Crypt depth ratio of duodenum and jejunum was significantly (pAloe supplementation in chicks as compared to their combination and control. Immediate post hatch supplementation of Aloe juice and neem extract in chicks improved the development and health of their gut.

  17. Green synthesis and characterization of silver nanoparticle using Aloe barbadensis

    Energy Technology Data Exchange (ETDEWEB)

    Thappily, Praveen, E-mail: pravvmon@gmail.com, E-mail: shiiuvenus@gmail.com; Shiju, K., E-mail: pravvmon@gmail.com, E-mail: shiiuvenus@gmail.com [Laboratory for Molecular Photonics and Electronics (LAMP), Department of Physics, National Institute of Technology, Calicut, Kerala 673601 (India)

    2014-10-15

    Green synthesis of silver nanoparticles was achieved by simple visible light irradiation using aloe barbadensis leaf extract as reducing agent. UV-Vis spectroscopic analysis was used for confirmation of the successful formation of nanoparticles. Investigated the effect of light irradiation time on the light absorption of the nanoparticles. It is observed that upto 25 minutes of light irradiation, the absorption is linearly increasing with time and after that it becomes saturated. Finally, theoretically fitted the time-absorption graph and modeled a relation between them with the help of simulation software.

  18. Green synthesis and characterization of silver nanoparticle using Aloe barbadensis

    Science.gov (United States)

    Thappily, Praveen; Shiju, K.

    2014-10-01

    Green synthesis of silver nanoparticles was achieved by simple visible light irradiation using aloe barbadensis leaf extract as reducing agent. UV-Vis spectroscopic analysis was used for confirmation of the successful formation of nanoparticles. Investigated the effect of light irradiation time on the light absorption of the nanoparticles. It is observed that upto 25 minutes of light irradiation, the absorption is linearly increasing with time and after that it becomes saturated. Finally, theoretically fitted the time-absorption graph and modeled a relation between them with the help of simulation software.

  19. Effect of dietary aloe vera on growth and lipid peroxidation indices in rainbow trout (Oncorhynchus mykiss).

    Science.gov (United States)

    Golestan, Ghazale; Salati, Amir Parviz; Keyvanshokooh, Saeed; Zakeri, Mohammad; Moradian, Hossein

    2015-01-01

    Aloe vera has been used worldwide in pharmaceutical, food and cosmetic industries due to the plethora of biological activities of its constituents. This study was done to evaluate the effects of dietary aloe vera on growth and lipid peroxidation in rainbow trout (Oncorhynchus mykiss). A total number of 480 O. mykiss (mean weight 9.50 ± 0.85 g) were randomized into four experimental groups including one control and three experimental groups that aloe vera was incorporated in their diet at 0.5, 1.0 and 2.0 g kg(-1). Trial was done for eight weeks. Then biometry and blood sampling were done. Plasma malondialdehyde, ferric reducing ability of plasma and growth index were estimated at the end of study. The results showed that aloe vera extract did not affect growth indices. Malondialdehyde was increased in the experimental group compared to the control group but ferric reducing ability of plasma showed a decrease in experimental groups (p aloe vera have adverse effects on antioxidant defense system in O. mykiss.

  20. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

    Science.gov (United States)

    Winters, Wallace D.; Scott, Michael; David, Andrew; Gillis, Glenn; Stoufflet, Thaya; Nair, Anand; Kousoulas, Konstantine

    2013-01-01

    Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL) aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks. PMID:23554812

  1. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

    Directory of Open Access Journals (Sweden)

    Inder Sehgal

    2013-01-01

    Full Text Available Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

  2. Toxicologic assessment of a commercial decolorized whole leaf aloe vera juice, lily of the desert filtered whole leaf juice with aloesorb.

    Science.gov (United States)

    Sehgal, Inder; Winters, Wallace D; Scott, Michael; David, Andrew; Gillis, Glenn; Stoufflet, Thaya; Nair, Anand; Kousoulas, Konstantine

    2013-01-01

    Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL) aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies-examined grossly and microscopically-that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

  3. Physicochemical characterization of microwave assisted synthesis of silver nanoparticles using Aloe Vera (Aloe barbadensis)

    Science.gov (United States)

    Kuponiyi, Abiola John

    Biosynthesis of silver nanoparticles (AgNP) using different biological extracts is gaining recognition for its numerous applications in different disciplines. Although different approaches (physical and chemical) have been used for the synthesis of AgNP, the green chemistry method is most preferable because of its high efficacy, cost effectiveness, and environmental benignity. Aloe Vera (AV) contains chemical compounds (anthraquinones) that are known to possess antibacterial, antivirus and anticancer properties and the extract is a good chemical reduction agent for AgNP. Hence, it was hypothesized that a microwave assisted synthesis will produce highly concentrated, homogeneous, stable and biologically active AgNP. Thus, the main objective of the study was to evaluate the effect of microwave assisted synthesis of AgNP, the effect of pulse laser treatment on size reduction of a microwave synthesized AgNP, and the physicochemical characterization of AgNP synthesized with Aloe Vera water and ethanol extract. The experiment was conducted in two phases. Phase 1 was first conducted to optimize the experimental variables, thus establishing the optimum variables to apply in the second phase. The experiment in Phase 1 was conducted using three-factor factorial experimental design comprised of the following factors: 1) Extraction Solvent, 2) Heating Methods, 3) pH; and their corresponding levels were water and ethanol, conventional and microwave, pH (7, 8, 10 and 12), respectively. All synthesis was conducted at constant temperature of 80°C. Phase II experimental treatments were Laser ablation (0, 5, and 10 min) and Storage time (Week 1, 2 & 3). The Phase I of the results showed that increased AgNP concentrations were significantly (p 0.05) impact the particle size distribution. Hence, the Zeta potential of the particles has values typically ranging between +100 mV to -100 mV, hence indicative of colloidal stability matrix. Furthermore, the Polydispersity indexes of Week 1

  4. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Buerki, Sven; Symonds, Matthew RE

    2015-01-01

    Background: Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world’s largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between...... the rich traditions of medicinal use and leaf succulence in aloes. Results: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major...... succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue...

  5. Anti-tumor activity of Aloe vera against DMBA/croton oil-induced skin papillomagenesis in Swiss albino mice.

    Science.gov (United States)

    Saini, M; Goyal, Pradeep Kumar; Chaudhary, Geeta

    2010-01-01

    Human populations are increasingly exposed to various carcinogens such as chemicals, radiation, and viruses in the environment. Chemopreventive drugs of plant origin are a promising strategy for cancer control because they are generally nontoxic or less toxic than synthetic che-mopreventive agents, and can be effective at different stages of carcinogenesis. The present investigation was undertaken to explore the antitumor activity of topical treatment with aloe vera (Aloe vera) gel, oral treatment with aloe vera extract, and topical and oral treatment with both gel and extract in stage-2 skin carcinogenesis in Swiss albino mice induced by 7,12-dim ethylbenz(a)anthracene (DMBA) and promoted croton (Croton tiglium) oil. The animals were randomly divided into 4 groups and treated as follows: Group I, DMBA + croton oil only (controls); Group II, DMBA + croton oil + topical aloe vera gel; Group III, DMBA + croton oil + oral aloe vera extract; Group I V, DMBA + croton oil + topical aloe vera gel + oral aloe vera extract. Results showed that body weight was significantly increased from 78.6% in the control group (Group I) to 92.5%, 87.5%, and 90.0% in Groups II, III, and I V, respectively. A 100% incidence of tumor development was noted in Group I, which was decreased to 50%, 60%, and 40% in Groups II, III, and I V, respectively. Also in Groups II, III, and IV, the cumulative number of papillomas was reduced significantly from 36 to 12, 15, and 11; tumor yield from 3.6 to 1.2, 1.5, and 1.1; and tumor burden from 3.6 to 2.4, 2.50, and 2.75, respectively, after treatment with aloe vera. Conversely, the average latent period increased significantly from 4.9 (Group I) to 5.23, 5.0, and 6.01 weeks in Groups II, III, and I V, respectively. We conclude that aloe vera protects mice against DMBA/croton oil-induced skin papillomagenesis, likely due to the chemopreventive activity of high concentrations of antioxidants such as vitamins A, C, and E; glutathione peroxidase; several

  6. TLR2 Signaling Pathway in Alveolar Bone Osteogenesis Induced by Aloe vera and Xenograft (XCB).

    Science.gov (United States)

    Kresnoadi, Utari; Rahayu, Retno Pudji; Rubianto, M; Sudarmo, Subijanto Marto; Budi, Hendrik Setia

    2017-01-01

    The aim of this study was to find the role of TLR2 signaling pathway in reducing osteoclast activity and promoting osteoblast growth by inducing a combination of Aloe vera and cancellous bovine xenograft (XCB) into dental extraction socket. Forty-eight Cavia cobayas were used. They were divided into eight groups (n=6). For control group, their mandibular incisors were extracted and filled with PEG. For treatment groups, they were extracted and filled with XCB, Aloe vera and the combination of Aloe vera and XCB. The first four groups were sacrificed after 7 days and the other groups after 30 days. Immunohistochemistry and histopathology examination were conducted to examine TLR2, TNFa, OPG, collagen-1, and the osteoblast and osteoclast expressions. The expressions of TLR2, OPG and Collagen-1, as well as the number of osteoblast were increased. Meanwhile, the expressions of TNFa and osteoclast were decreased. The study finding was that TLR2 signaling pathway influenced alveolar bone osteogenesis process by reducing osteoclast activity and stimulating osteoblast growth induced by the combination of Aloe vera and XCB.

  7. UJI AKTIVITAS KOMBINASI EKSTRAK AIR LIDAH BUAYA ( Aloe vera L. DAN AKAR MANIS (Glycyrrhiza glabra L. SEBAGAI PENYUBUR RAMBUT

    Directory of Open Access Journals (Sweden)

    Sulistiorini Indriaty

    2016-05-01

    Full Text Available Aloe vera (Aloe vera L. contains amino acids, vitamins, folic acid, and liquorice (Glycyrrhiza glabra L containing glisirizin, and flavonoids, which have properties as fertilizer hair. This study aims to obtain a combination of extracts with activity most optimal fertilizer hair, made 3 combination with a combination of 1 (the water extract of liquorice concentration of 2.5% and 7.5% aloe vera, a combination of 2 (the water extract of liquorice concentrations of 2, 5% and 2.5% aloe vera, and combinations of 3 (the water extract of liquorice concentration of 5% and 7.5% aloe vera. Testing the activity of hair growth is done by measuring the rate of hair growth, hair growth acceleration and weight of rabbit hair for 28 days. Rabbit hair growth results showed the combination 1 has an average length of the largest hair with hair growth rate value of 2.97 cm, accelerated hair growth amounted to 0.15 cm / day on day 7 and weighing 0.13 gram hair.

  8. A multi-analytical approach for the identification of aloe as a colorant in oil-resin varnishes.

    Science.gov (United States)

    Rizzo, Adriana; Shibayama, Nobuko; Kirby, Daniel P

    2011-03-01

    Aloe plants have been widely documented in artists' treatises dating from the sixteenth to the nineteenth century as a source of colorant to achieve lustrous golden glazes on tin- and silver-foiled objects and warm-toned finishes on musical instruments, such as violins. Aloe extracts contain characteristic anthraquinone and phenolic components which impart a distinctive orange tone and fluorescence to mixtures containing them. Because of the low concentration of colorant in the coatings and its probable degradation by high temperature during manufacture, the identification of aloe in heated oil-resin mixtures represents an analytical challenge. For this reason, the possible presence of aloe in glazes and coatings has been largely overlooked. This paper describes various analytical approaches to the identification of aloe in historic samples, from comparison with results obtained from reference standards and mock-up samples. Complementary analytical techniques including thermally assisted hydrolysis and methylation-gas chromatography-mass spectrometry, high-performance liquid chromatography, laser desorption-mass spectrometry, matrix-assisted laser desorption-ionization-mass spectrometry and surface-enhanced Raman scattering were used. Different chemical markers were identified by the individual methods and the advantages and limitations of each technique for the identification of aloe in oil-resin varnishes are discussed.

  9. Physicochemical characterization of silver nanoparticles synthesize using Aloe Vera (Aloe barbadensis)

    Science.gov (United States)

    Kuponiyi, Abiola; Kassama, Lamin; Kukhtareva, Tatiana

    2014-08-01

    Production of silver nanoparticles (AgNPs) using different biological methods is gaining recognition due to their multiple applications. Although, several physical and chemical methods have been used for the synthesis and stabilizing of AgNPs, yet, a green chemistry method is preferable because it is cost effective and environmentally friendly. The synthesis was done using Aloe Vera (AV) extract because it has chemical compounds such as "Antrokinon" that are known for its antibacterial, antivirus and anticancer properties. We hypothesize that AV extract can produce a stable nanoparticles within the 100 nm range and be biologically active. The biological compounds were extracted from AV skin with water and ethanol which was used as the reduction agent for the synthesis of nanoparticles. The biological extract and AgNO3 were blended and heated to synthesize AgNPs. The reaction process was monitored using UV-Visible spectroscopy. Fourier Transfer Infrared spectroscopy (FTIR) was used for the characterization of biological compounds and their substituent groups before and after the reaction process. Dynamic Light scattering (DLS) method was used to characterize particle size of AgNPs and their biomolecular stability. Results showed that biological compounds such as aliphatic amines, alkenes (=C-H), alkanes (C-H), alcohol (O-H) and unsaturated esters(C-O), which has an average particle size of 109 and 215.8 nm and polydispersity index of 0.451 and 0.375 for ethanol and water extract, respectively. According to TEM measurements the size of AgNPs are in the range 5-20 nm The results suggested that ethanol derived AgNPs contained higher yield of organic compounds, thus has better solubility power than water. Ag NPs can be used to control salmonella in poultry industry.

  10. Aloe vera Induced Biomimetic Assemblage of Nucleobase into Nanosized Particles

    Science.gov (United States)

    Chauhan, Arun; Zubair, Swaleha; Sherwani, Asif; Owais, Mohammad

    2012-01-01

    Aim Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, biomimetic (employing microorganism/plants) synthesis of metal and inorganic materials nano-particles has emerged as a simple and viable strategy. In the present study, we have extended biological synthesis of nano-particles to organic molecules, namely the anticancer agent 5-fluorouracil (5-FU), using Aloe vera leaf extract. Methodology The 5-FU nano- particles synthesized by using Aloe vera leaf extract were characterized by UV, FT-IR and fluorescence spectroscopic techniques. The size and shape of the synthesized nanoparticles were determined by TEM, while crystalline nature of 5-FU particles was established by X-ray diffraction study. The cytotoxic effects of 5-FU nanoparticles were assessed against HT-29 and Caco-2 (human adenocarcinoma colorectal) cell lines. Results Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the nano-assembled 5-FU retained its anticancer action against various cancerous cell lines. Conclusion In the present study, we have explored the potential of biomimetic synthesis of nanoparticles employing organic molecules with the hope that such developments will be helpful to introduce novel nano-particle formulations that will not only be more effective but would also be devoid of nano-particle associated putative toxicity constraints. PMID:22403622

  11. ACID HIDROLYSIS OF ALOE VERA (SABILA BAGASSE FOR OBTAINING PECTIN

    Directory of Open Access Journals (Sweden)

    Caridad Curbelo Hernández

    2017-04-01

    Full Text Available The processing of Aloe vera (sábila in sp. has reached a great development during the last years, since its products are raw material for other industries. This has contributed to the increase of solid residues, which are not utilized to obtain added value products in the same industry. In this research, the acid hydrolysis method for pectin extraction from the Aloe vera bagasse was studied. Firstly, the bagasse was characterized determinig: humidity, ashes, proteins, acidity, and total content of pectin, density and pH. pH, temperature, extraction time, particle size and speed of agitation were analyzed as variables of study or factors and pectin yield (% as response variable. The best results were obtained at 90°C, 1000 min-1 and the larger particle size that were the significant variables with 95 % of confidence. Pectin was characterized chemically and for infrared spectroscopy (FTIR. It was concluded that this pectin was of fast gellification variety, water-soluble and of high methoxyl content. The characteristic peaks of the functional groups of pectin were observed in infrared spectroscopy (FTIR analysis.

  12. Acute toxicity and laxative activity of Aloe ferox resin

    Directory of Open Access Journals (Sweden)

    Vanessa R. L. Celestino

    2013-02-01

    Full Text Available Aloe ferox Mill., Xanthorrhoeaceae, resin is the solid residue obtained by evaporating the latex that drains from the leaves transversally cut. Aloe ferox has been used in folk medicine as anti-inflammatory, immunostimulant, anti-bacterial, anti-fungal, antitumor, laxative and to heal wounds and burns. The effects of the oral administration of A. ferox resin (10, 25, 50, 100 and 200 mg/kg were evaluated on intestinal transit in mice and its acute toxicity (5.0 g/kg in Wistar rats. The hydroxyanthracene derivatives present in the resin were expressed as aloin, identified by thin layer chromatography and quantified by spectrophotometry. The aloin (Rf 0.35 was identified and the percentage of hydroxyanthracene derivates expressed as aloin was 33.5%. A. ferox resin extract (50, 100 and 200 mg/kg increased the gastrointestinal motility at a 30 min interval at 93.5, 91.8 and 93.8%, respectively, when compared to control group (46.5%. A single oral dose of the A. ferox resin extract did not induce signs of toxicity or death. Thus, the results demonstrate that A. ferox has laxative activity and that it is nontoxic, since LD50 could not be estimated and it is possibly higher than 5.0 g/kg.

  13. Acute toxicity and laxative activity of Aloe ferox resin

    Directory of Open Access Journals (Sweden)

    Vanessa R. L. Celestino

    2013-04-01

    Full Text Available Aloe ferox Mill., Xanthorrhoeaceae, resin is the solid residue obtained by evaporating the latex that drains from the leaves transversally cut. Aloe ferox has been used in folk medicine as anti-inflammatory, immunostimulant, anti-bacterial, anti-fungal, antitumor, laxative and to heal wounds and burns. The effects of the oral administration of A. ferox resin (10, 25, 50, 100 and 200 mg/kg were evaluated on intestinal transit in mice and its acute toxicity (5.0 g/kg in Wistar rats. The hydroxyanthracene derivatives present in the resin were expressed as aloin, identified by thin layer chromatography and quantified by spectrophotometry. The aloin (Rf 0.35 was identified and the percentage of hydroxyanthracene derivates expressed as aloin was 33.5%. A. ferox resin extract (50, 100 and 200 mg/kg increased the gastrointestinal motility at a 30 min interval at 93.5, 91.8 and 93.8%, respectively, when compared to control group (46.5%. A single oral dose of the A. ferox resin extract did not induce signs of toxicity or death. Thus, the results demonstrate that A. ferox has laxative activity and that it is nontoxic, since LD50 could not be estimated and it is possibly higher than 5.0 g/kg.

  14. Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens.

    Science.gov (United States)

    Akhtar, Masood; Hai, Abdul; Awais, Mian Muhammad; Iqbal, Zafar; Muhammad, Faqir; ul Haq, Ahsan; Anwar, Muhammad Irfan

    2012-05-25

    This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (pcoccidiosis in industrial broiler chickens. Copyright © 2011 Elsevier B.V. All rights reserved.

  15. ALOS Palser 1.0 Radar Processing System

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Americas ALOS Data Node (AADN) With the Japan Aerospace Exploration Agency's (JAXA's) launch of the Advanced Land Observation Satellite (ALOS) in January 2006, a new...

  16. ALOS Palsar 1.1 Radar Processing System

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Americas ALOS Data Node (AADN) with the Japan Aerospace Exploration Agency's (JAXA's) launch of the Advanced Land Observation Satellite (ALOS) in January 2006, a new...

  17. ALOS Palsar 1.5 Radar Processing System

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Americas ALOS Data Node (AADN) With the Japan Aerospace Exploration Agency's (JAXA's) launch of the Advanced Land Observation Satellite (ALOS) in January 2006, a new...

  18. The effect of Aloe vera gel on viability of dental pulp stem cells.

    Science.gov (United States)

    Sholehvar, Fatemeh; Mehrabani, Davood; Yaghmaei, Parichehr; Vahdati, Akbar

    2016-10-01

    Dental pulp stem cells (DPSCs) can play a prominent role in tissue regeneration. Aloe vera L. (Liliaceae) contains the polysaccharide of acemannan that was shown to be a trigger factor for cell proliferation, differentiation, mineralization, and dentin formation. This study sought to determine the viability of DPSCs in Aloe vera in comparison with Hank's balanced salt solution (HBSS). Twelve rabbits underwent anesthesia, and their incisor teeth were extracted; the pulp tissue was removed, chopped, treated with collagenase and plated in culture flasks. DPSCs from passage 3 were cultured in 24-well plates, and after 3 days, the culture media changed to 10, 25, 50, and 100% concentrations of Aloe vera at intervals of 45 and 90 min and 3 and 6 h. Distilled water was used as negative and HBSS as positive control for comparison. The cell morphology, viability, population doubling time (PDT), and growth kinetics were evaluated. RT-PCR was carried out for characterization and karyotyping for chromosomal stability. Aloe vera showed a significant higher viability than HBSS (74.74%). The 50% Aloe vera showed higher viability (97.73%) than other concentrations. PDT in 50% concentration was 35.1 h and for HBSS was 49.5 h. DPSCs were spindle shaped and were positive for CD73 and negative for CD34 and CD45. Karyotyping was normal. Aloe vera as an inexpensive and available herb can improve survival of avulsed or broken teeth in emergency cases as a transfer media. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  19. Acceleration of the alcohol oxidation rate in rats with aloin, a quinone derivative of Aloe.

    Science.gov (United States)

    Chung, J H; Cheong, J C; Lee, J Y; Roh, H K; Cha, Y N

    1996-11-08

    Aloe contains abundant aloin, a C-glycoside derivative of anthraquinone. Based on recent reports indicating that the water extract of Aloe enhances the ethanol oxidation rate and also that quinones, in general, have a functional role in elevating the alcohol oxidation rate in vivo, we have attempted to identify the quinone derivative contained in Aloe that could increase the alcohol oxidation rate. Upon oral administration of aloin (300 mg/kg) given 12 hr prior to the administration of alcohol (3.0 g/kg), the blood alcohol area under the curve (AUC) was found to be decreased significantly (by 40%). This was supported by increases in the rates of blood alcohol elimination and the disappearance of alcohol from the body by 45 and 50%, respectively. Analysis of hepatic triglyceride (TG) levels revealed that both the ethanol and the aloin treatment alone significantly increased the TG levels in a comparable manner; however, the level obtained by the combined treatment of aloin and ethanol was not statistically different from that produced by either treatment alone. The levels of serum L-aspartate:2-oxoglutarate aminotransferase (AST) and L-alanine:2-oxoglutarate aminotransferase (ALT) activities were not increased by acute alcohol intoxication, aloin alone, or by the combined treatment of alcohol and aloin. Pretreatments with aloe-emodin, the anthraquinone aglycone of aloin, resulted in a significantly decreased blood alcohol AUC and an increase in the rate of ethanol disappearance. These results suggested that when the aloin localized primarily in the skin of Aloe is ingested, aloe-emodin (the quinone aglycone) may be released, and the released quinone may produce acceleration of the ethanol metabolism rate in vivo.

  20. An HPLC Procedure for the Quantification of Aloin in Latex and Gel from Aloe barbadensis Leaves.

    Science.gov (United States)

    Sánchez-Machado, Dalia I; López-Cervantes, Jaime; Mariscal-Domínguez, María F; Cruz-Flores, Paola; Campas-Baypoli, Olga N; Cantú-Soto, Ernesto U; Sanches-Silva, Ana

    2017-03-01

    Aloin is an anthraquinone-C-glycoside present in Aloe vera. This compound is extremely variable among different species and highly depends on the growing conditions of the plants. The quantification of aloin in different extraction preparations has been a frequent problem due to the high instability of the compound. The aim of the present study is to develop and validated an analytical method for aloin detection in fresh and dry samples of Aloe barbadensis gel and latex using high performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Phosphate buffered saline (pH 3) was selected as the extraction solvent. The aloin was separated using a Zorbax Eclipse AAA column (4.6 × 150 mm) at 35°C, and water and acetonitrile were used as the mobile phase at a flow rate of 0.9 mL/min. The linearity was satisfactory with a correlation coefficient greater than 0.999. Under these conditions, the method precision (relative standard deviation) was 3.71% for FL, 4.41% for dry latex, 0.81% for fresh gel and 4.42% for dry gel samples. Aloe latex was determined to have a greater amount of aloin than aloe gel. The method validation was satisfactory and exhibited adequate linearity, repeatability and accuracy. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)

    AJB SERVER

    identification of Aloin A and Aloe emodium has already been achieved in A. excelsa (Coopoosamy and Magwa,. 2006) Chrysophanol is known to occur in both commercial viable species of aloes (Aloe vera and A. ferox), but has not previously been isolated from A. excelsa. MATERIALS AND METHODS. Measurements.

  2. Role of Natural Immunomodulator (Aloe Vera in Cellular and Humoral Immune Responses

    Directory of Open Access Journals (Sweden)

    Ening Wiedosari

    2007-12-01

    Full Text Available Aloe vera belongs to a group of Liliaceae family plant and cultivated worldwide. It possesses acemannan (acetylated mannan, which has a significant pharmacological property. The acemannan has an immunomodulatory activity when administered to animals. The major immunomodulating effect includes the activation of immune effector cells, such as lymphocytes and macrophages, resulting in the production of cytokines, interleukin (IL-1, IL-6, IL-12 and tumor necrosis factor alpha (TNFα. In particular, this extract can modulate the differentiation capacity of CD4+T cells to mature into Th1 subsets and enhance the innate cytokine response. As a consequence, this extract will have a profound effect in controlling disease, caused by intracellular infectious agents (bacteria and viruses. However, further studies are needed to determine the immunomodulating effects of Aloe vera in multi-component extracts equivalent to what are being used commonly in traditional medicine.

  3. Aloe vera: It's effect on gingivitis.

    Science.gov (United States)

    Ajmera, Neha; Chatterjee, Anirban; Goyal, Vikas

    2013-07-01

    Aloe vera is the oldest medicinal plant that has maintained its popularity over the course of time. It is widely known for its medicinal uses in wound healing, as an analgesic, and for its anti-inflammatory properties. The aim of this study is to evaluate the anti-inflammatory property of aloe vera mouthwash on plaque-induced gingivitis. Forty-five patients who were diagnosed with plaque-induced gingivitis were included in the study. They were divided into three groups with fifteen patients in each group. Group 1 was asked to rinse with 10 ml of aloe vera mouthwash twice daily for three months. Group 2 were treated with scaling only. Group 3 patients were asked to rinse with aloe vera mouthwash and scaling was done. The clinical changes were evaluated with Loe and Silness gingival index (1963) and Muhlemann and Son's Sulcus bleeding index (1971) at baseline, after one month and three months, respectively. The data obtained was compared statistically. The paired 't' test was done for intragroup comparison and one-way analysis of variance with a post hoc Tukey test was used for intergroup comparison. The data was obtained at the baseline, end of first month, and end of the third month. The result suggested reduction in gingival inflammation in all the three groups, but it was more in the aloe vera mouthwash and scaling group. Hence, it was concluded that aloe vera had a significant anti-inflammatory property. Thus, it can be used as an adjunct to mechanical therapy for treating plaque-induced gingivitis.

  4. Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera

    Directory of Open Access Journals (Sweden)

    Rubina Lawrence

    2009-12-01

    Full Text Available The ethanol, methanol and acetone extracts of Aloe vera gel were studied for their antimicrobial activity against four Gram-positive and Gram-negative bacteria using agar well diffusion method. The extracts showed varied levels of antimicrobial activity against the tested pathogens. The ethanol and methanol extracts showed higher activity while acetone extract, showed least or no activity against most of the tested pathogens. Fractions obtained from the extracts by Thin Layer and Column Chromatography were studied for their antagonistic properties using Spot Assay Technique. Compounds with maximum antibacterial activity isolated from the ethanol and methanol extracts were identified as p - coumaric acid (Mol. wt.165, ascorbic acid (Mol. wt.177 , pyrocatechol (Mol. wt.110 and cinnamic acid (Mol. wt.148, on the basis of Gas Chromatography Mass Spectrometry. The study suggests the antimicrobial activity of the A. vera gel extract to be dependant on the synergistic effect of different compounds. With the broad spectral antimicrobial effect of A. vera gel, it could be further recommended in the treatment of various bacterial diseases.

  5. The Effects of Aloe Vera on TNF-a Levels, the Percentage of Nk Cells and Th 17 Cells in Rat That Received Izoniazid and Rifampycin.

    Science.gov (United States)

    Mawarti, Herin; Rajin, Mukhamad; Asumta, Zulfikar

    2017-10-01

    The present study was undertaken to investigate the hepatoprotective effect of Aloe vera against side effect of antituberculosis drug. Twenty-five rats will be divided into five groups, namely the control group (without any treatment), the group of rats treated with anti-tuberculosis drugs, and a group of rats were treated antituberculosis drugs and got Aloe vera extract at a dose of 40; 80; and 120 mg/kg body weight. Antituberculosis drugs are isoniazid and rifampicin a dose of 50 mg/kg body weight. Antituberculosis treated group showed significantly increase levels of TNF-a, the percentage of NK cells and the number of Th17 cells compared with the control group (p Aloe vera reduce levels of TNF-a compared with the antituberculosis group (p 0.05). Aloe vera at first and the third dose lower the number of NK cells compared to the antituberculosis group, although it has not yet reached a significant difference (p > 0.05). The first dose of Aloe vera was significantly decreased the percentage of Th17 cells compared to the antituberculosis drug group (p 0.05). It was concluded that administration of Aloe vera can suppress the production of TNF-a and the percentage of Th17 cells as a result of antituberculosis drug administration. Thus, Aloe vera can be a useful alternative to natural materials in the successful treatment of tuberculosis through the inhibition of side effect.

  6. Bio-prospecting endemic Mascarene Aloes for potential neuroprotectants.

    Science.gov (United States)

    Lobine, D; Howes, M-J R; Cummins, I; Govinden-Soulange, J; Ranghoo-Sanmukhiya, M; Lindsey, K; Chazot, P L

    2017-12-01

    The Mascarene Aloes are used in the traditional pharmacopoeia against various ailments including cutaneous diseases and as antispasmodics. Scientific evidence to support these claims is non-existent and mainly based on the scientific repute of A. vera. The antioxidant profile of methanolic leaf extracts of A. purpurea Lam., A. tormentorii (Marais) L. E. Newton & G. D. Rowley, A. lomatophylloides Balf. f., A. macra Haw. and A. vera (L.) Burm. f. was studied using the total antioxidant capacity, copper equivalent and superoxide dismutase assays. In vitro cytotoxicity was evaluated on CAD (Cath.-a-differentiated) neuronal cells by the methyl tetrazolium assay, and the neuroprotective profile was assessed using hydrogen peroxide-induced neurotoxicity with the CAD cells. The aloin and vitexin content were determined by high-performance liquid chromatography with diode-array detection. A. purpurea had the highest aloin content (546.6 nmol/g), while A. tormentorii had the highest vitexin content (67.3 nmol/g). A. macra (concentration aloin and vitexin content that are also present in other reputed medicinal Aloes. Copyright © 2017 John Wiley & Sons, Ltd.

  7. Efek Pemberian Ekstrak Lidah Buaya (Aloe Barbadensis Miller pada Soket Gigi terhadap Kepadatan Serabut Kolagen Pasca Ekstraksi Gigi Marmut (Cavia Porcellus

    Directory of Open Access Journals (Sweden)

    Fatma Yuza

    2014-12-01

    sockets density of collagen were observed using light microscope 400x magnification. Analyzing data is done by Kruskal-Wallis test followed by Post Hoc test using the Mann-Whitney test for comparing collagen density between groups. Statistically results between groups showed that the extract of 90% Aloe barbadensis Miller affected the formation of collagen fibers when compared to the control group (p <0.05 on day 7 after tooth extraction of guinea pig. The conclusion of this study was 90% Aloe barbadensis Miller extract increased the density of collagen fibers from the tooth socket seven days after tooth extraction of guinea pig.

  8. Clinical Effectiveness of Aloe Vera in the Management of Oral Mucosal Diseases- A Systematic Review

    Science.gov (United States)

    Nair, Gopakumar Ramachandran; Naidu, Giridhar Seetharam; Jain, Supreet; Makkad, Ramanpal Singh; Jha, Abhishek

    2016-01-01

    Introduction Aloe vera is well known for its medicinal properties which lead to its application in treating various diseases. Its use in treating oral lesions has not been much documented in literature. Aim Although, systematic reviews on aloe vera and its extracts have been done earlier, but in relation to oral diseases this is the first systematic review. The aim of the present systematic review was to compile evidence based studies on the effectiveness of Aloe vera in treatment of various oral diseases. Materials and Methods Computerized literature searches were performed to identify all published articles in the subject. The following databases were used: PUBMED [MEDLINE], SCOPUS, COCHRANE DATABASE, EMBASE and SCIENCE DIRECT using specific keywords. The search was limited to articles published in English or with an English Abstract. All articles (or abstracts if available as abstracts) were read in full. Data were extracted in a predefined fashion. Assessment was done using Jadad score. Results Fifteen studies satisfied the inclusion criteria. Population of sample study ranged from 20 patients to 110 patients with clinically diagnosed oral mucosal lesions. Out of 15 studies, five were on patients with oral lichen planus, two on patients with oral submucous fibrosis, other studies were carried on patients with burning mouth syndrome, radiation induced mucositis, candida associated denture stomatitis, xerostomic patients and four were on minor recurrent apthous stomatitis. Most studies showed statistically significant result demonstrating the effectiveness of Aloe vera in treatment of oral diseases. Conclusion Although there are promising results but in future, more controlled clinical trials are required to prove the effectiveness of Aloe vera for management of oral diseases. PMID:27656587

  9. Clinical Effectiveness of Aloe Vera in the Management of Oral Mucosal Diseases- A Systematic Review.

    Science.gov (United States)

    Nair, Gopakumar Ramachandran; Naidu, Giridhar Seetharam; Jain, Supreet; Nagi, Ravleen; Makkad, Ramanpal Singh; Jha, Abhishek

    2016-08-01

    Aloe vera is well known for its medicinal properties which lead to its application in treating various diseases. Its use in treating oral lesions has not been much documented in literature. Although, systematic reviews on aloe vera and its extracts have been done earlier, but in relation to oral diseases this is the first systematic review. The aim of the present systematic review was to compile evidence based studies on the effectiveness of Aloe vera in treatment of various oral diseases. Computerized literature searches were performed to identify all published articles in the subject. The following databases were used: PUBMED [MEDLINE], SCOPUS, COCHRANE DATABASE, EMBASE and SCIENCE DIRECT using specific keywords. The search was limited to articles published in English or with an English Abstract. All articles (or abstracts if available as abstracts) were read in full. Data were extracted in a predefined fashion. Assessment was done using Jadad score. Fifteen studies satisfied the inclusion criteria. Population of sample study ranged from 20 patients to 110 patients with clinically diagnosed oral mucosal lesions. Out of 15 studies, five were on patients with oral lichen planus, two on patients with oral submucous fibrosis, other studies were carried on patients with burning mouth syndrome, radiation induced mucositis, candida associated denture stomatitis, xerostomic patients and four were on minor recurrent apthous stomatitis. Most studies showed statistically significant result demonstrating the effectiveness of Aloe vera in treatment of oral diseases. Although there are promising results but in future, more controlled clinical trials are required to prove the effectiveness of Aloe vera for management of oral diseases.

  10. Acemannan and Fructans from Aloe vera (Aloe barbadensis Miller) Plants as Novel Prebiotics.

    Science.gov (United States)

    Quezada, Maria Paz; Salinas, Carlos; Gotteland, Martin; Cardemil, Liliana

    2017-11-22

    The nutraceutical properties of Aloe vera have been attributed to a glucomannan known as acemannan. Recently information has been published about the presence of fructans in Aloe vera but there are no publications about acemannan and fructans as prebiotic compounds. This study investigated in vitro the prebiotic properties of these polysaccharides. Our results demonstrated that fructans from Aloe vera induced bacterial growth better than inulin (commercial FOS). Acemannan stimulated bacterial growth less than fructans, and as much as commercial FOS. Using qPCR to study the bacterial population of human feces fermented in a bioreactor simulating colon conditions, we found that fructans induce an increase in the population of Bifidobacterium spp. Fructans produced greater amounts of short chain fatty acids (SCFA), while the branched-chain fatty acids (BCFA) did not increase with these polysaccharides. Acemannan increased significantly acetate concentrations. Therefore, both Aloe vera polysaccharides have prebiotic potentials.

  11. Skin permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox.

    Science.gov (United States)

    Fox, Lizelle T; Gerber, Minja; du Preez, Jan L; du Plessis, Jeanetta; Hamman, Josias H

    2015-01-01

    The aim of this study was to investigate the in-vitro permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox using ketoprofen as a marker compound. The permeation studies were conducted across excised female abdominal skin in Franz diffusion cells, and the delivery of ketoprofen into the stratum corneum-epidermis and epidermis-dermis layers of the skin was investigated using a tape-stripping technique. A. vera gel showed the highest permeation-enhancing effect on ketoprofen (enhancement ratio or ER = 2.551) when compared with the control group, followed by A. marlothii gel (ER = 1.590) and A. ferox whole-leaf material (ER = 1.520). Non-linear curve fitting calculations indicated that the drug permeation-enhancing effect of A. vera gel can be attributed to an increased partitioning of the drug into the skin, while A. ferox whole leaf modified the diffusion characteristics of the skin for ketoprofen. The tape stripping results indicated that A. marlothii whole leaf delivered the highest concentration of the ketoprofen into the different skin layers. Of the selected aloe species investigated, A. vera gel material showed the highest potential as transdermal drug penetration enhancer across human skin. © 2014 Royal Pharmaceutical Society.

  12. Effect of Aloe vera gel on antimicrobial and sensory properties of ultra-filtered white cheese

    Directory of Open Access Journals (Sweden)

    K. Sajadi

    2017-05-01

    Full Text Available Aloe vera gel contains a blend of carbohydrates, enzymes, vitamins and minerals, and has antimicrobial and anti-oxidant properties. In this study, after extraction and homogenization of Aloe vera gel, the effect of various concentrations (0.5, 1, 2, 5, 10 and 15% of the gel was investigated on flavor  microbial flora (total microbial count and mesophilic lactic acid bacteria and also its inhibitory effect on Penicillium citrinum (PTCC 5304 in cheese. Results of sensory evaluation showed that cheeses produced with the concentrations of 0.5 and 1% gel had the highest acceptance. Moreover, it was revealed that in the control sample the number of total count and lactic acid bacteria increased in from 1 to 3 months of storage; meanwhile in the gel-containing samples the microbial populations reduced during the same period.In addition, Aloe vera gel at the concentration of 15% caused 37.3% inhibition of P. citrinum in. It was concluded that some concentration of Aloe vera gel could retard the growth of P. citrinum without sensory defects.

  13. The effects of Aloe vera (Aloe barbadensis) coating on the quality of shrimp during cold storage.

    Science.gov (United States)

    Soltanizadeh, Nafiseh; Mousavinejad, Mohsen S

    2015-10-01

    Green tiger shrimp (Penaeus semisulcatus) is an important aquaculture species worldwide. Its perishable nature, however, needs preservation methods to ensure its quality and shelf life. In this study, the effects of Aloe vera coating on the quality and shelf life of shrimps during cold storage were investigated. Shrimp samples were dipped in aqueous solutions containing 25%, 50%, 75%, and 100% Aloe vera gel before storage at 4 °C for 7 days. Drip loss, pH, TBA, TVB-N, and texture of both the control and treated shrimp samples were analyzed periodically. There were significant differences between coated shrimps and the control group in all parameters evaluated. Aloe vera at 75% and 100% concentrations was able to prevent lipid oxidation and drip loss properly; however, coatings containing 25% Aloe vera did not have the desired effects on these characteristics. Shrimps coated with higher concentrations of Aloe vera had better textural properties during cold storage. Results also indicated the positive effects of Aloe vera coating on the sensory quality of shrimp.

  14. Fast Dissolving Tablets of Aloe Vera Gel

    African Journals Online (AJOL)

    Erah

    combined effect of two formulation variables - amounts of microcrystalline cellulose and mannitol. Results: The results of multiple regression analysis revealed that in order to obtain a fast dissolving tablet of the Aloe vera gel, an optimum concentration of mannitol and a higher content of microcrystalline cellulose should be ...

  15. ORGANOGÉNESIS FLORAL EN ACNISTUS ARBORESCENS, DUNALIA SOLANACEA, DEPREA BITTERIANA, LARNAX GLABRA Y LARNAX HAWKESII - TRIBU PHYSALEAE (SOLANACEAE

    Directory of Open Access Journals (Sweden)

    GARZÓN-VENEGAS JAVIER

    2006-12-01

    Full Text Available Se estudió la organogénesis floral en las especies Acnistus arborescens, Dunaliasolanacea, Deprea bitteriana, Larnax glabra y Larnax hawkesii de la tribuPhysaleae (Solanaceae, empleando microscopía electrónica de barrido en modoambiental. Para fines de comparación de la organogénesis de las cinco especies, setuvieron en cuenta tres estados de desarrollo. Las especies estudiadas comparten lasecuencia acrópeta en la que surgen sépalos, pétalos, estambres y carpelos, así comoel arreglo simultáneo de corola y androceo. Comparten también la simpetalia tardía,siendo aún más tardía en las especies de Larnax. El tiempo de fusión de los pétalospuede considerarse un carácter importante para la diferenciación entre Deprea yLarnax. Se encontraron diferencias intergenéricas en cuanto al arreglo del cáliz, quees simultáneo en Dunalia solanacea, mientras que en Deprea bitteriana y en lasdos especies de Larnax es espiralado, en A. arborescens el patrón de desarrollo delos sépalos es intermedio. Los carpelos se desarrollan fusionados basalmente en unestadio temprano, todas las especies son bicarpelares, sin embargo, el gineceo de A.arborescens da la apariencia de ser tricarpelar por la formación de un “falso septo”.

  16. The effect of Aloe vera A. Berger (Liliaceae) on gastric acid secretion and acute gastric mucosal injury in rats.

    Science.gov (United States)

    Yusuf, Sadiq; Agunu, Abdulkarim; Diana, Mshelia

    2004-07-01

    The effect of varying doses of ethanol extract of Aloe vera (Liliaceae) on acute gastric mucosal lesions induced by 0.6 M HCl and acid output was studied in the pylorus ligated and lumen perfuse rats, respectively. Acid secretion was determined by titration of the collected gastric juice to pH 7.0. Intraperitoneal injection of Aloe vera, dose dependently inhibited gastric acid secretion. The plant was more active as a gastroprotective agent at lower concentration against mucosal injury induced by 0.6 M HCl. In conclusion, Aloe vera is endowed with gastric acid anti-secretory activity and could protect the gastric mucosa at low concentrations against injurious agents.

  17. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera.

    Science.gov (United States)

    Grace, Olwen M; Buerki, Sven; Symonds, Matthew R E; Forest, Félix; van Wyk, Abraham E; Smith, Gideon F; Klopper, Ronell R; Bjorå, Charlotte S; Neale, Sophie; Demissew, Sebsebe; Simmonds, Monique S J; Rønsted, Nina

    2015-02-26

    Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world's largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between the rich traditions of medicinal use and leaf succulence in aloes. The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major radiations driven by different speciation processes, giving rise to the extraordinary diversity known today. Large, succulent leaves typical of medicinal aloes arose during the most recent diversification ~10 million years ago and are strongly correlated to the phylogeny and to the likelihood of a species being used for medicine. A significant, albeit weak, phylogenetic signal is evident in the medicinal uses of aloes, suggesting that the properties for which they are valued do not occur randomly across the branches of the phylogenetic tree. Phylogenetic investigation of plant use and leaf succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue, an adaptive feature that likely contributed to the ecological success of the genus Aloe, is the main predictor for medicinal use among Aloe species, whereas evolutionary loss of succulence tends to be associated with losses of medicinal use. Phylogenetic analyses of plant use offer potential to understand patterns in the value of global plant diversity.

  18. Efficacy of early chick nutrition with Aloe vera and Azadirachta indica on gut health and histomorphometry in chicks

    Directory of Open Access Journals (Sweden)

    Tamilvanan Sujatha

    2017-06-01

    Full Text Available Aim: This study was conducted with an aim of studying the efficacy of water supplements of Aloe vera and Azadirachta indica (neem during pre-starter age (0-2 weeks on gut health and histomorphometry in Vanaraja chicks. Materials and Methods: A total of 192 day old Vanaraja chicks were randomly assigned to one of four herbal water treatments throughout the experimental pre-starter stage (0-2 weeks in a completely randomized design. Each treatment was given four replicates consisting of 12 chicks per replicate. Water treatments comprised T1: Control with regular antibiotic supplement, T2: 3 ml Aloe juice per chick per day, T3: 3 ml neem extract per chick per day, T4: 1.5 ml Aloe and 1.5 ml neem per chick per day. Gut culture was done for Escherichia coli and Lactobacillus sps. and gut histomorphometry in 24 gut samples at 14 days of age. Results: This study revealed that supplementation of A. vera and neem in water significantly (p<0.05 reduced and increased the number of gut E. coli and Lactobacillus sps. Colonies, respectively, as compared to that of control groups; Villi was significantly (p<0.05 taller and broader on 14 days of age across the jejunum of chicks fed with neem supplementation as compared to that of control chicks. Significantly lower crypt depth (p<0.05 was observed in the duodenum of Aloe supplementation. Villus height: Crypt depth ratio of duodenum and jejunum was significantly (p<0.05 increased neem and Aloe supplementation in chicks as compared to their combination and control. Conclusion: Immediate post hatch supplementation of Aloe juice and neem extract in chicks improved the development and health of their gut.

  19. In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications

    Science.gov (United States)

    Fox, Lizelle T.; du Plessis, Jeanetta; Gerber, Minja; van Zyl, Sterna; Boneschans, Banie; Hamman, Josias H.

    2014-01-01

    Objective: To investigate the skin hydrating and anti-erythema activity of gel materials from Aloe marlothii A. Berger and A. ferox Mill. in comparison to that of Aloe barbadensis Miller (Aloe vera) in healthy human volunteers. Materials and Methods: Aqueous solutions of the polisaccharidic fractions of the selected aloe leaf gel materials were applied to the volar forearm skin of female subjects. The hydration effect of the aloe gel materials were measured with a Corneometer® CM 825, Visioscan® VC 98 and Cutometer® dual MPA 580 after single and multiple applications. The Mexameter® MX 18 was used to determine the anti-erythema effects of the aloe material solutions on irritated skin areas. Results: The A. vera and A. marlothii gel materials hydrated the skin after a single application, whereas the A. ferox gel material showed dehydration effects compared to the placebo. After multiple applications all the aloe materials exhibited dehydration effects on the skin. Mexameter® readings showed that A. vera and A. ferox have anti-erythema activity similar to that of the positive control group (i.e. hydrocortisone gel) after 6 days of treatment. Conclusion: The polysaccharide component of the gel materials from selected aloe species has a dehydrating effect on the skin after multiple applications. Both A. vera and A. ferox gel materials showed potential to reduce erythema on the skin similar to that of hydrocortisone gel. PMID:24991119

  20. EVALUATION OF FREEZE DRIED ALOE VERA AND NOPAL CACTUS FOR POSSIBLE HEALTH TREATMENTS BY COMPARISON OF ANTIOXIDANT PROPERTIES AND FREE RADICAL INHIBITION.

    Science.gov (United States)

    Rodriguez, Kenneth R; Jones, Anthony E; Belmont, Barbara

    2014-01-01

    The goal of this project was to characterize the antioxidant powers of lyophilized Aloe Vera (Aloe barbadensis) and Nopal Cactus (Opuntia ficus-indica) by quantifying the phenolics content and radical scavenging abilities of preparations derived from these plants. Extracts of these lyophylized succulents were assayed for phenolic compounds by the Folin Ciocalteau method and compared for free radical scavenging capability by the DPPH method. We found that even though the Aloe lyophilizate extract contained more phenolic content, the Nopal lyophilizate exhibited better free radical scavenging ability. Aloe Vera extract contained 0.278 g/L of phenolic content and exhibited 11.1% free radical inhibition, with a free radical scavenging rate constant of 0.177±0.015 min-1. Nopal Cactus extract contained 0.174 g/L of phenolic content and exhibited 13.2% free radical inhibition, with a free radical scavenging rate constant of 0.155±0.009 min-1. These results showed Nopal to have greater antioxidant potency than Aloe.

  1. Simultaneous Estimation of Aloe Emodin and Emodin from Rheum emodi, Cassia alata and Aloes by HPTLC.

    Science.gov (United States)

    Narayanan, Sindhu; Jadhav, Aruna P

    2015-01-01

    A simple, precise, specific, accurate high performance thin layer chromatography method was developed for simultaneous estimation of aloe emodin and emodin from medicinal plants like Rheum emodi (Rhubarb), Barbados aloes (dried juice of Aloe barbadensis leaf) and Cassia alata (Candle bush). Thin layer chromatographic aluminum plates pre-coated with silica gel 60 F254 was used as the stationary phase for chromatographic separation of the drugs. Toluene:ethyl acetate:formic acid (10:2:1 v/v/v) was selected as mobile phase and analysis was carried out in absorbance mode at iso-absorptive wavelength of 263 nm. This method shows good resolution for both drugs with retention factor 0.37±0.03 and 0.55±0.03 for aloe emodin and emodin, respectively. The regression analysis data indicated good linear relationship for the calibration plots for aloe emodin and emodin in the range of 300 - 800 ng/spot and 150 - 400 ng/spot and regression coefficient was 0.9993 and 0.9994, respectively. Validation of the method was performed according to International Conference on Harmonisation guidelines for following parameters: Accuracy, precision, limit of detection, linearity, limit of quantification, robustness and specificity. In conclusion, the developed method was found to be rapid, simple, reliable and specific for the identification and quantitation of these anthraquinones in medicinal plants and marketed formulations.

  2. Design, formulation and evaluation of Aloe vera chewing gum

    OpenAIRE

    Aslani, Abolfazl; Ghannadi, Alireza; Raddanipour, Razieh

    2015-01-01

    Background: Aloe vera has antioxidant, antiinflammatory, healing, antiseptic, anticancer and antidiabetic effects. The aim of the present study was to design and evaluate the formulation of Aloe vera chewing gum with an appropriate taste and quality with the indications for healing oral wounds, such as lichen planus, mouth sores caused by cancer chemotherapy and mouth abscesses as well as reducing mouth dryness caused by chemotherapy. Materials and Methods: In Aloe vera powder, the carbohydra...

  3. Aloe vera-based formula as emollient on horses' hooves

    Directory of Open Access Journals (Sweden)

    Samuel Pereira Simonato

    2013-06-01

    Full Text Available The present study aimed at developing an Aloe vera-based formula for topical use on horse hoof and evaluating whether the treatment affects hooves growth and balance. Six healthy male horses between the ages of 3 and 17 years (12±5.25 were used, all semi-confined animals for breeding purposes. Before beginning A. vera treatment, animals underwent two trimming procedures with a 45 days-interval. After the second trimming, one of the forelimbs and one of the hindlimbs of 4 horses was weekly treated by topical application of the glycolic extract of A. vera at 20%. The contralateral limb, randomly chosen, received the extract at 50%. The hooves of the other animals were treated with propylene glycol. Treatment was done for 225 days and, during this time, animals underwent periodic trimming. Variables related to growth and balance of the hooves were measured before and after trimming. Data were analyzed using chi-square test and regression analysis at 5% significance. Growth rate of the hooves was not related to treatment. On the other hand, the 50% extract was related to the majority of the hooves in balance (p<0.05. Results suggest that a weekly topical treatment with A. vera glycolic extract does not improve the growth rate of the hooves; however, when applied at a high concentration, it improves their balance.

  4. Unraveling the medicinal potential of South African Aloe species.

    Science.gov (United States)

    Amoo, Stephen O; Aremu, Adeyemi O; Van Staden, Johannes

    2014-04-11

    Aloe species (family: Xanthorrhoeaceae) are widely used in ethnomedicine for treating various ailments. Approximately 125 Aloe taxa are documented as indigenous to South Africa. This review was aimed at critically evaluating the available data on the ethnomedicinal uses, pharmacological activities, safety and conservation status of the documented South African Aloe species. A detailed search using major electronic search engines (such as Google Scholar, Scopus and Scirus) and ethnobotanical literature was undertaken. Search terms used included 'medicinal properties of Aloe species', 'biological activity of South African Aloe species' and 'safety and toxicological evaluation of aloes'. Although Aloe species are widely used as laxatives and for treating wound and skin-related ailments, only about 20% of South African Aloe species have been clearly documented for their medicinal uses. The pharmacological potential including the antimicrobial, antiinflammatory, antiplasmodial and anthelmintic activities of some of the species has been established, providing a rationale for their use in traditional medicine. Successful micropropagation protocols have been developed as a conservation strategy, but only for a few species. The highlighted medicinal activities of some Aloe species indicate their therapeutic potential. Nonetheless, further research especially on the understudied species is required to properly document their ethnomedicinal uses and fully explore their pharmacological value. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  5. Memristors in the electrical network of Aloe vera L

    National Research Council Canada - National Science Library

    Volkov, Alexander G; Reedus, Jada; Mitchell, Colee M; Tucket, Clayton; Forde-Tuckett, Victoria; Volkova, Maya I; Markin, Vladislav S; Chua, Leon

    2014-01-01

    .... Here we found that memristors exist in vivo. The electrostimulation of the Aloe vera by bipolar sinusoidal or triangle periodic waves induce electrical responses with fingerprints of memristors...

  6. Ugala meenutab Alo Mattiisenit Viljandis naeruoratooriumiga / Riina Oruaas

    Index Scriptorium Estoniae

    Oruaas, Riina

    2001-01-01

    16. aug. Viljanis esietenduvast kontsertetendusest "Roheline muna". Teatraliseeritud kontsertlavastus põhineb Alo Mattiiseni ja Peeter Volkonski samanimelisel risotooriumil, lavastaja Andres Lepik, kunstnik Jaak Vaus

  7. [Determination of aloin in aloes by HPLC].

    Science.gov (United States)

    Cao, Hong; Liu, Yun

    2003-04-01

    To describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes. The analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm. The calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6). The results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin.

  8. Aloe vera as cure for lichen planus.

    Science.gov (United States)

    Patil, Bharati A; Bhaskar, Hebbar Pragati; Pol, Jyoti S; Sodhi, Amandeep; Madhu, Asha V

    2013-01-01

    Oral lichen planus is a difficult condition to treat because of its chronic nature. Various treatment modalities have resulted in partial regression of symptoms but not a complete cure. Aloe vera, a product with minimal adverse effects, can be tried to treat this disorder. A 38-year-old male patient diagnosed with lichen planus of the skin and the oral mucosa was suffering from severe pain and a burning sensation intraorally and pruritus of the skin lesions. Considering the extensive involvement, an herbal alternative was considered. The patient was prescribed aloe vera juice and gel application for two months. At the nine-month follow-up, the patient was symptom-free and totally cured of the intraoral and skin lesions.

  9. Antiviral activity of Aloe hijazensis against some haemagglutinating viruses infection and its phytoconstituents.

    Science.gov (United States)

    Abd-Alla, Howaida I; Abu-Gabal, Nagat S; Hassan, Amal Z; El-Safty, Mounir M; Shalaby, Nagwa M M

    2012-08-01

    Evaluation of the antiviral activities of flowers, flower-peduncles, leaves, and roots of Aloe hijazensis against haemagglutinating viruses of avian paramyxovirus type-1 (APMV-1), avian influenza virus type A (AI-H5N1), Newcastle disease virus (NDV), and egg-drop syndrome virus (EDSV) in specific pathogen free (SPF) chicken embryos were carried out. Extract of the flowers and leaves showed relatively higher activity than the extracts of other plant parts. Thirteen compounds were isolated from both the flowers and flower-peduncles of A. hijazensis. The isolated compounds were classified into: five anthraquinones; ziganein, ziganein-5-methyl ether, aloesaponarin I, chrysophanol, aloe-emodin, one dihydroisocoumarin; feralolide, four flavonoids; homoplantaginin, isoorientin, luteolin 7-glucuronopyranoside, isovitexin, one phenolic acid; p-coumaric acid, the anthrone; barbaloin together with aloenin. Eleven compounds were attributed to the flowers and seven to the flower-peduncles. Homoplantaginin and luteolin 7-glucuronopyranoside are reported here for the first time from Aloe spp. To the best of our knowledge, this is the first report on the chemical composition and biological activity of those plant parts.

  10. Formulation development, optimization and evaluation of aloe vera gel for wound healing

    Science.gov (United States)

    Khan, Abdul Wadood; Kotta, Sabna; Ansari, Shahid Hussain; Sharma, Rakesh Kumar; Kumar, Amit; Ali, Javed

    2013-01-01

    Purpose: To formulate and optimize a herbal gel of Aloe vera extract containing Carbopol 934 as gelling agent and to investigate the effects of topical application of Carbopol 934 gel containing Aloe vera extract on the healing of skin wounds surgically induced in Wistar rats. Materials and Methods: Different concentrations of viscosity enhancer Carbopol 934 were tried and finally gel that showed good spreadability and consistency was selected for wound healing property of herbal gel of Aloe vera. Excision wound model was used for the study. Results: The optimized gel was evaluated for different physicochemical properties and wound healing property. Differences in wound healing were observed between the various treatments when compared to the control group. Tissue hyperplasia was lower in the control group compared to the other treated groups. In animals group treated with gel, 80.14% healing was observed up to 14th day. While in untreated group I (control) animals showed 52.68% healing of wounds on 14th day. On the other hand, control group animals also showed inflammation and pus formation up to 5th day of study, while treated animals did not showed any observable inflammation and pus formation. Conclusion: Results shows prepared gel has promising effect on the wound healing process. PMID:24143047

  11. Impedance studies of a green blend polymer electrolyte based on PVA and Aloe-vera

    Science.gov (United States)

    Selvalakshmi, S.; Mathavan, T.; Vijaya, N.; Selvasekarapandian, Premalatha, M.; Monisha, S.

    2016-05-01

    The development of polymer electrolyte materials for energy generating and energy storage devices is a challenge today. A new type of blended green electrolyte based on Poly-vinyl alcohol (PVA) and Aloe-vera has been prepared by solution casting technique. The blending of polymers may lead to the increase in stability due to one polymer portraying itself as a mechanical stiffener and the other as a gelled matrix supported by the other. The prepared blend electrolytes were subjected to Ac impedance studies. It has been found out that the polymer film in which 1 gm of PVA was dissolved in 40 ml of Aloe-vera extract exhibits highest conductivity and its value is 3.08 × 10-4 S cm-1.

  12. Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge.

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; Ma, Xin Yu; He, Jie; Xu, Pao; Liu, Kai

    2015-10-01

    The current study investigated the effects of dietary Aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of GIFT-tilapia juveniles under Streptococcus iniae challenge. Five dietary groups were designed including a control and 100 % Aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. Fish fed dietary Aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced (P Aloe/kg feed compared to unsupplemented ones. High-density lipoprotein was significantly elevated in fish fed 0.5 and 1 % Aloe/kg feed compared to unsupplemented ones, and no significant changes (P > 0.05) were noted in low-density lipoprotein among test groups. Furthermore, high activities of superoxide dismutase, catalase, and glutathione peroxide in liver tissues were observed in Aloe-supplemented fish compared to unsupplemented ones, before and after S. iniae challenge (7.7 × 10(6) CFU cells/mL). Variations were also noted in malondialdehyde activity throughout the trial, but no significant difference (P > 0.05) was observed between groups. Meanwhile, Aloe-supplemented fish reduced serum aspartate and alanine aminotransferase (AST and ALT) activities before and after challenge. Based on the second-order polynomial regression analysis, dietary Aloe inclusion levels less than or equal to 1.88, 1.86, and 2.79 %/kg feed were determined to be suitable in improving plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia in this study, respectively. Thus, A. vera extracts may be recommended as a tilapia feed supplement to enhance fish antioxidant and hepatoprotective capacities, especially during disease outbreaks.

  13. In vitro activity of Aloe vera inner gel against Helicobacter pylori strains.

    Science.gov (United States)

    Cellini, L; Di Bartolomeo, S; Di Campli, E; Genovese, S; Locatelli, M; Di Giulio, M

    2014-07-01

    Aloe barbadensis Miller (Aloe vera) is a herbal remedy widely used for a variety of illnesses; A. vera leaf extracts have been promoted for detoxification, cure constipation, help flush out toxins and wastes from the body, promote digestion and are used in the treatment of peptic ulcer for cytoprotective action. The aim of this study was to evaluate the antibacterial activity of A. vera inner gel against both susceptible and resistant Helicobacter pylori strains isolated in Abruzzo region, Italy. The inner gel of leaves of a 5-year-old plant of A. vera was extracted, homogenized and tested from 800 to 1.56 mg ml(-1) against 14 clinical strains and one reference strain of H. pylori using the broth microdilution methodology. Furthermore, the sample of A. vera was investigated for the chemical fingerprint of anthraquinones. The inhibitory concentrations of A. vera inner gel were similar to the bactericidal ones, with values ranging from 6.25 to 800 mg ml(-1) . Fifty per cent of the detected strains, independently of their susceptibility profile, were inhibited in their growth at 100 mg ml(-1) . Aloe vera inner gel expresses antibacterial properties against H. pylori and, therefore, in combination with antibiotics, could represent a novel strategy for the treatment of the infection of H. pylori, especially in cases of multiresistance. The study demonstrates that the Aloe vera inner gel expresses antibacterial properties against both susceptible and resistant Helicobacter pylori strains. These findings may impact on the antimicrobial resistance phenomenon of H. pylori, proposing the A. vera inner gel as a novel effective natural agent for combination with antibiotics for the treatment of H. pylori gastric infection. © 2014 The Society for Applied Microbiology.

  14. Three-Arm Randomized Phase III Trial: Quality Aloe and Placebo Cream Versus Powder as Skin Treatment During Breast Cancer Radiation Therapy.

    Science.gov (United States)

    Hoopfer, Donna; Holloway, Caroline; Gabos, Zsolt; Alidrisi, Maha; Chafe, Susan; Krause, Barbara; Lees, Alan; Mehta, Nirmal; Tankel, Keith; Strickland, Faith; Hanson, John; King, Charlotte; Ghosh, Sunita; Severin, Diane

    2015-06-01

    The efficacy of aloe extract in reducing radiation-induced skin injury is controversial. The purpose of the present 3-arm randomized trial was to test the efficacy of quality-tested aloe extract in reducing the severity of radiation-induced skin injury and, secondarily, to examine the effect of a moist cream versus a dry powder skin care regimen. A total of 248 patients with breast cancer were randomized to powder, aloe cream, or placebo cream. Acute skin toxicity was scored weekly and after treatment at weeks 1, 2, and 4 using a modified 10-point Catterall scale. The patients scored their symptom severity using a 6-point Likert scale and kept an acute phase diary. The aloe formulation did not reduce acute skin toxicity or symptom severity. Patients with a greater body mass index were more likely to develop acute skin toxicity. A similar pattern of increased skin reaction toxicity occurred with both study creams compared with the dry powder regimen. No evidence was found to support prophylactic application of quality aloe extract or cream to improve the symptoms or reduce the skin reaction severity. Our results support a dry skin care regimen of powder during radiation therapy. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. Three dosimetry models of lipoma arborescens treated by {sup 90}Y synovectomy

    Energy Technology Data Exchange (ETDEWEB)

    O’Doherty, Jim, E-mail: jim.odoherty@kcl.ac.uk [Department of Medical Physics-Nuclear Medicine, Royal Surrey County Hospital, Guildford GU2 7XX, United Kingdom and Division of Imaging Sciences, PET Imaging Centre at St. Thomas’ Hospital, King' s College London, London SE1 7EH (United Kingdom); Clauss, Ralf [Department of Nuclear Medicine, Royal Surrey County Hospital, Guildford GU2 7XX (United Kingdom); Scuffham, James [Department of Medical Physics-Nuclear Medicine, Royal Surrey County Hospital, Guildford GU2 7XX (United Kingdom); Khan, Aman [Department of Rheumatology, Royal Surrey County Hospital, Guildford GU2 7XX (United Kingdom); Petitguillaume, Alice; Desbrée, Aurélie [Service de Dosimétrie Interne, Institut de Radioprotection et de Sûreté Nucléaire, 92260 Fontenay-aux-Roses (France)

    2014-05-15

    Purpose: Lipoma arborescens (LA) is a benign intra-articular lipomatous proliferation of the synovial membrane. This extremely rare condition has previously been treated by intra-articular{sup 90}Y radiosynoviorthesis but dosimetry literature on this form of radionuclide therapy is nonexistent. The authors detail methodology for successful treatment of LA and provide for the first time estimates of radiation dosimetry. The authors also analyze the biodistribution of the radiopharmaceutical over the course of the patient's treatment through sequential imaging. Methods: A patient with bilateral LA underwent intracavity injection of{sup 90}Y citrate colloid to the right and left knee joint spaces (181 and 198 MBq, respectively). SPECT/CT datasets were acquired over 9 days to quantify the biodistribution and kinetics of the radiopharmaceutical. Radiation dosimetry was performed using the MIRD schema (through OLINDA software), a custom voxel-based method, and a direct Monte Carlo calculation (OEDIPE). Results: Follow-up MRI showed marked reduction in LA size in both knees. Mean absorbed doses to the LA were 21.2 ± 0.8 and 42.9 ± 2.3 Gy using OLINDA, 8.1 ± 0.3 and 16.7 ± 0.5 Gy using voxel based methodology, and 8.2 ± 0.3 and 15.7 ± 0.5 Gy for OEDIPE in the right and left LA, respectively. Distribution of the radiopharmaceutical within the joint space alters over the imaging period, with less than 1% of the remaining activity having moved posteriorly in the knee cavity. No uptake was detected outside of the joint space after assessment with whole-body scintigraphy. Conclusions: An activity of approximately 185 MBq successfully relieved clinical symptoms of LA. There was good correlation between direct Monte Carlo and voxel based techniques, but OLINDA was shown to overestimate the absorbed dose to the tumor. Accurate dosimetry may help select an activity more tailored to the specific size and location of the LA.

  16. Arizona Lesson Observation and Evaluation (ALOE): Design Test Edition, 1990.

    Science.gov (United States)

    Fargo, J. Steven; And Others

    The Arizona Lesson Observation and Evaluation (ALOE) system of evaluating teaching is presented. ALOE was developed from Arizona adaptations of the Georgia Teacher Performance Assessment Instruments and, with G. Taylor's "Functional Elements Analysis of Teaching Skills" (FEATS), forms an integrated observation package which allows…

  17. Isolation of volatile compounds of Aloe excelsa (Berger)

    African Journals Online (AJOL)

    hope&shola

    2010-10-25

    Oct 25, 2010 ... carried out on Aloe ferox, Aloe vera as well as A. excelsa. (Speranza et al., 1986, 1990; Koyama et al., 1994; Eloff,. 1998; Amabeoku et al., 1998; Dagne, 2000; Coopoosamy and Magwa 2007). This investigation deals with the isola- tion of volatile compounds present in the leaf exudates of. A. excelsa.

  18. Fast Dissolving Tablets of Aloe Vera Gel | Madan | Tropical Journal ...

    African Journals Online (AJOL)

    Purpose: The objective of this work was to prepare and evaluate fast dissolving tablets of the nutraceutical, freeze dried Aloe vera gel. Methods: Fast dissolving tablets of the nutraceutical, freeze-dried Aloe vera gel, were prepared by dry granulation method. The tablets were evaluated for crushing strength, disintegration ...

  19. The Foliar Anatomy And Micromorphology Of Aloe Ferox Mill ...

    African Journals Online (AJOL)

    Background: The genus Aloe has been the subject of several chemotaxonomic and phytochemical investigations. Aloe ferox is an important source of biologically active compounds like anthrones, chromones, pyrones, and flavonoids. It is a plant used extensively in traditional medicine. Despite the myriad of studies on the ...

  20. DAYA HAMBAT EKSTRAK KULIT DAUN LIDAH BUAYA (Aloe barbadensis Miller TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 25923DAN Escherichia coli ATCC 25922

    Directory of Open Access Journals (Sweden)

    Ariyanti Ni Kadek

    2013-04-01

    Full Text Available This research aimed to determine the inhibited effect of Aloe (Aloe barbadensis Miller rind extract to the growthof bacteria Staphylococcus aureus and Escherichia coli and the most effective concentration for the resistanceagainst both of bacteria tested. The results showed that the extracts of Aloe rind inhibited the growth of bacteriaStaphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. It was indicated by the formation ofinhibition zones with the largest diameter average occurred concentrations of 100% with the size 11,58 mm forthe Staphylococcus aureus and 6,81 mm in Escherichia coli. The highest concentration inhibited the growth ofStaphylococcus aureus ATCC 25923 was 100% and 75% for the Escherichia coli ATCC 25922.

  1. Safety of purified decolorized (low anthraquinone) whole leaf Aloe vera (L) Burm. f. juice in a 3-month drinking water toxicity study in F344 rats.

    Science.gov (United States)

    Shao, A; Broadmeadow, A; Goddard, G; Bejar, E; Frankos, V

    2013-07-01

    Decolorized (purified, low anthraquinone) whole leaf Aloe vera (L.) Burm. f. juice was administered at concentrations of 0%, 0.5%, 1% and 2% in the drinking water of F344Du rats for 3 months without any adverse effect. The no-observed-adverse-effect level (NOAEL) in this study was considered to be >2%w/v (>1845 mg/kg bodyweight/day for males and >2920 mg/kg bodyweight for females). The test material contained total anthraquinones at Aloe vera extracts tested in other studies have resulted in an increased incidence and severity of diarrhea and colon adenomas and carcinomas. The results of this study supports the assertion that the high levels of anthraquinone present in orally administered, non-purified whole leaf Aloe vera extract may be responsible for the adverse effects observed on the colon. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Evaluation of aloin and aloe-emodin as anti-inflammatory agents in aloe by using murine macrophages.

    Science.gov (United States)

    Park, Mi-Young; Kwon, Hoon-Jeong; Sung, Mi-Kyung

    2009-04-23

    The aloe ingredients responsible for physiological effects and the concentrations required to exert their biological activities are not fully understood. This study compares the anti-inflammatory effects of aloin and aloe-emodin with other polyphenols. Our results demonstrated that aloe-emodin dose-dependently inhibited inducible nitric oxide synthase (iNOS) mRNA expression and nitric oxide (NO) production at 5-40 microM. In addition, the levels of cyclooxygenase-2 (COX-2) mRNA and prostaglandin E2 (PGE2) production were suppressed by 40 microM aloe-emodin. Aloin also suppressed the production of NO at 5-40 microM, although it did not suppress PGE2 production. The present results indicate that aloin and aloe-emodin possibly suppress the inflammatory responses by blocking iNOS and COX-2 mRNA expression. The anti-inflammatory effect of aloe-emodin was comparable to that of kaempferol and quercetin, indicating aloe-emodin as a possible key constituent responsible for the anti-inflammatory activity of aloe.

  3. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress.

    Science.gov (United States)

    Salinas, Carlos; Handford, Michael; Pauly, Markus; Dupree, Paul; Cardemil, Liliana

    2016-01-01

    Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC). There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC) in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

  4. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

    Directory of Open Access Journals (Sweden)

    Pushkala Ramachandran

    2014-01-01

    Full Text Available Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG and papaya. Aloe gel (30%, papaya pulp (15%, spice extract (5%, and citric acid (0.1% were mixed in given proportion to prepare the blend with TSS of 15 °Brix. The product was bottled, pasteurized, and stored at room temperature. The quality characteristics and storage stability of the spiced beverage blend (SAGPB were compared with spiced papaya RTS beverage (SPB. Periodic analysis was carried out up to five months for various physicochemical parameters, sugar profile, bioactive compounds, microbial quality, instrumental color, and sensory acceptability. The SAGPB exhibited superior quality characteristics compared to SPB both in fresh and in stored samples. The SPB was acceptable up to four months and SAGPB for five months. The results indicate that nutraceutical rich AG could be successfully utilized to develop functional fruit beverages with improved quality and shelf life.

  5. Phytochemical screening of the exudate of Aloe otallensis and its effect on Leishmania donovani

    Directory of Open Access Journals (Sweden)

    Zerihun Tesfaye Nigusse

    2016-06-01

    Full Text Available Objective: To evaluate the antileishmanial activity of methanolic extract of Aloe otallensis (A. otallensis on the promastigote stage of Leishmania donovani (L. donovani as compared to standard drugs and to screen its phytochemical constituents. Methods: Phytochemical screening was done by using the method mentioned by Evans and Trease on methanolic extract of the exudates of Aloe otallensis leaves. The extract was also evaluated for in vitro antileishmanial activity against L. donavani which is found from the Parasitology Unit of Black Lion Hospital. The result was compared to standard drugs of sodium stibogluconate, milfostin and paramomycin. Results: The extract has a good antileishmanial activity with an IC50 of 0.1230 μg/mL on L. donovani (AM 563. The experimental data showed that relatively it had better activity than paramomycin and milfostin but less activity than sodium stibogluconate. The data analyses were done by GraphPad Prism version 5 software after it was read by ELISA reader at the wave length of 650 nm. The phytochemical screening of the exudates of A. otallensis showed the presence of phenol, alkaloid and saponin. Conclusions: The methanol extract of the exudates of A. otallensis has a good antileishmaniasis activity and this may be attributed to phenol, alkaloid and saponin present in the plant. But it needs further analysis for the conformation of which constituent presents in high concentration to know which one has the strongest effect.

  6. Antioxidant and Cytotoxicological Effects of Aloe vera Food Supplements

    Directory of Open Access Journals (Sweden)

    Zaira López

    2017-01-01

    Full Text Available Currently, food industries use supplements from Aloe vera as highly concentrated powders (starting products, which are added to the final product at a concentration of 1x, meaning 10 g/L for decolourized and spray-dried whole leaf powder (WLP or 5 g/L for decolourized and spray-dried inner leaf powder (ILG and also for nondecolourized and belt-dried inner leaf powder (ILF. Flavonoids, tannins, or saponins could not be detected for any starting product at this concentration and their total phenol concentration of 68–112 μM gallate-eq. was much lower than in fresh extract; however, their antioxidant capacity of 90–123 μM ascorbate-eq. for DPPH was similar to the fresh extract. Starting products, dissolved at 1x, had an aloin concentration of 0.04 to 0.07 ppm, a concentration much lower than the industry standard of 10 ppm for foodstuff. While decolourized starting products (i.e., treated with activated carbon exhibited low cytotoxicity on HeLa cells (CC50 = 15 g/L ILG or 50 g/L WLP, ILF at CC50 = 1–5 g/L exhibited cytotoxic effects, that is, at concentrations even below the recommended for human consumption. Probable causes for the cytotoxicity of ILF are the exposure to high temperatures (70–85°C combined with a high fibre content.

  7. Identification of A. arborescens, A. grandis, and A. protenta as new members of the European Alternaria population on potato.

    Science.gov (United States)

    Landschoot, Sofie; Vandecasteele, Michiel; De Baets, Bernard; Höfte, Monica; Audenaert, Kris; Haesaert, Geert

    2017-02-01

    Alternaria species, primarily the small-spored Alternaria alternata and the large-spored Alternaria solani, are considered a serious threat to potato cultivation. To develop control strategies, it is important to gain insight into the Alternaria population. Based on the sequence analyses of the internal transcribed spacer region (ITS) and the glyceraldehyde-3-phosphate dehydrogenase gene, the small-spored and large-spored Alternaria isolates could be separated from each other. Sequence analyses of the calmodulin gene and the RNA polymerase second largest subunit showed that besides A. solani also A. grandis and A. protenta were present in the large-spored Alternaria population. Sequence analyses of the Alternaria major allergen gene Alt a 1 and the elongation factor-α revealed that both A. alternata and species belonging to the Alternaria arborescens species complex were present in the small-spored Alternaria population. Furthermore, according to the histone h3 sequence the members of the A. arborescens species complex could be subdivided into two groups. Concerning the fitness, it was concluded that the mycelium growth rate of the large-spored isolates was significantly lower compared to the growth rate of the small-spored isolates. In contrast, the spore-germinating capacity and early growth of the large-spored isolates was greater compared to those of the small-spored isolates. Within the groups of small-spored and large-spored isolates there were no significant differences in fitness between the species. Copyright © 2016 British Mycological Society. All rights reserved.

  8. Anti-cancer stem cell activity of a sesquiterpene lactone isolated from Ambrosia arborescens and of a synthetic derivative.

    Directory of Open Access Journals (Sweden)

    Wendy Soria Sotillo

    Full Text Available New regimens are constantly being pursued in cancer treatment, especially in the context of treatment-resistant cancer stem cells (CSCs that are assumed to be involved in cancer recurrence. Here, we investigated the anti-cancer activity of sesquiterpene lactones (SLs isolated from Ambrosia arborescens and of synthetic derivatives in breast cancer cell lines, with a specific focus on activity against CSCs. The breast cancer cell lines MCF-7, JIMT-1, and HCC1937 and the normal-like breast epithelial cell line MCF-10A were treated with the SLs damsin and coronopilin, isolated from A. arborescens, and with ambrosin and dindol-01, synthesized using damsin. Inhibitory concentration 50 (IC50 values were obtained from dose-response curves. Based on IC50 values, doses in the μM range were used for investigating effects on cell proliferation, cell cycle phase distribution, cell death, micronuclei formation, and cell migration. Western blot analysis was used to investigate proteins involved in cell cycle regulation as well as in the NF-κB pathway since SLs have been shown to inhibit this transcription factor. Specific CSC effects were investigated using three CSC assays. All compounds inhibited cell proliferation; however, damsin and ambrosin were toxic at single-digit micromolar ranges, while higher concentrations were required for coronopilin and dindol-01. Of the four cell lines, the compounds had the least effect on the normal-like MCF-10A cells. The inhibition of cell proliferation can partly be explained by downregulation of cyclin-dependent kinase 2. All compounds inhibited tumour necrosis factor-α-induced translocation of NF-κB from the cytoplasm to the nucleus. Damsin and ambrosin treatment increased the number of micronuclei; moreover, another sign of DNA damage was the increased level of p53. Treatment with damsin and ambrosin decreased the CSC subpopulation and inhibited cell migration. Our results suggest that these compounds should be

  9. Anti-cancer stem cell activity of a sesquiterpene lactone isolated from Ambrosia arborescens and of a synthetic derivative.

    Science.gov (United States)

    Sotillo, Wendy Soria; Villagomez, Rodrigo; Smiljanic, Sandra; Huang, Xiaoli; Malakpour, Atena; Kempengren, Sebastian; Rodrigo, Gloria; Almanza, Giovanna; Sterner, Olov; Oredsson, Stina

    2017-01-01

    New regimens are constantly being pursued in cancer treatment, especially in the context of treatment-resistant cancer stem cells (CSCs) that are assumed to be involved in cancer recurrence. Here, we investigated the anti-cancer activity of sesquiterpene lactones (SLs) isolated from Ambrosia arborescens and of synthetic derivatives in breast cancer cell lines, with a specific focus on activity against CSCs. The breast cancer cell lines MCF-7, JIMT-1, and HCC1937 and the normal-like breast epithelial cell line MCF-10A were treated with the SLs damsin and coronopilin, isolated from A. arborescens, and with ambrosin and dindol-01, synthesized using damsin. Inhibitory concentration 50 (IC50) values were obtained from dose-response curves. Based on IC50 values, doses in the μM range were used for investigating effects on cell proliferation, cell cycle phase distribution, cell death, micronuclei formation, and cell migration. Western blot analysis was used to investigate proteins involved in cell cycle regulation as well as in the NF-κB pathway since SLs have been shown to inhibit this transcription factor. Specific CSC effects were investigated using three CSC assays. All compounds inhibited cell proliferation; however, damsin and ambrosin were toxic at single-digit micromolar ranges, while higher concentrations were required for coronopilin and dindol-01. Of the four cell lines, the compounds had the least effect on the normal-like MCF-10A cells. The inhibition of cell proliferation can partly be explained by downregulation of cyclin-dependent kinase 2. All compounds inhibited tumour necrosis factor-α-induced translocation of NF-κB from the cytoplasm to the nucleus. Damsin and ambrosin treatment increased the number of micronuclei; moreover, another sign of DNA damage was the increased level of p53. Treatment with damsin and ambrosin decreased the CSC subpopulation and inhibited cell migration. Our results suggest that these compounds should be further investigated

  10. Browse Title Index

    African Journals Online (AJOL)

    Items 1 - 50 of 11090 ... Vol 5, No 10 (2006), -Amylase production by Penicillium fellutanum isolated from mangrove rhizosphere soil, Abstract PDF ... Vol 10, No 20 (2011), Aloe arborescens aqueous gel extract alters the activities of key hepatic enzymes and blood concentration of triglycerides, glucose and insulin in ...

  11. Aloe activated P-glycoprotein and CYP 3A: a study on the serum kinetics of aloe and its interaction with cyclosporine in rats.

    Science.gov (United States)

    Yang, Meng-Syuan; Yu, Chung-Ping; Huang, Ching-Ya; Chao, Pei-Dawn Lee; Lin, Shiuan-Pey; Hou, Yu-Chi

    2017-01-25

    Aloe, the leaf juice of Aloe vera, is a popular functional food worldwide. The major constituents of aloe are polyphenolic anthranoids such as aloin, aloe-emodin and rhein. Cyclosporine (CSP), an immunosuppressant with a narrow therapeutic window, is a probe substrate of P-glycoprotein (P-gp), an efflux pump, and CYP 3A4. This study first investigated the serum kinetics of aloe, then evaluated the modulation effects of aloe on P-gp and CYP 3A through an aloe-CSP interaction study in rats. The serum kinetic study showed that aloe-emodin glucuronides (G) and rhein sulfates/glucuronides (S/G) were major molecules in the bloodstream. The aloe-CSP interaction study showed that the systemic exposure to CSP was significantly decreased by either a single dose or multiple doses of aloe. The results of in vitro studies indicated that aloe activated P-gp and aloe metabolites activated CYP 3A4. In conclusion, aloe ingestion activated the functions of P-gp and CYP 3A in rats.

  12. Hypoglycemic effect of instant aloe vera on the diabetic rats

    Directory of Open Access Journals (Sweden)

    Riyanto

    2017-09-01

    Full Text Available Instant aloe vera contains phenolic compounds which has antioxidative activity. However, this product is hygroscopic and damaged easily during storage. The critical condition of the instant occurs at the moisture content of 12.52 ± 0.24% (wb. Increasing the moisture content could accelerate oxidation of the phenolic compounds, thus decrease the antioxidative activity. Previous research showed that the antioxidative activity of instant aloe vera could lower the blood glucose. The purpose of this study was to evaluate the hypoglycemic activity of instant aloe vera during storage until the critical condition. The hypoglycemic effect was determined with the in vivo method using diabetic Wistar rats as experimental animals. The diabetic rats were fed with a standard feed combined with instant aloe vera which has been stored at various storage time i.e. 0, 2, 4, 6, 8 weeks and used normal rats fed without instant aloe vera as a control. The blood glucose was analyzed every week until 4 weeks. The research showed that the diabetic rats fed with standard feed without instant aloe vera had high blood glucose (219.40 mg/dL after 4 weeks treatment. Otherwise, the blood glucose of diabetic rats fed with instant aloe vera decreased from 214.00 mg/dL to 97.57 mg/dL after 4 weeks.

  13. Aloe vera Leaf Anti Inflamation’s Activity Speeds Up the Healing Proccess of Oral Mucosa Ulceration

    Directory of Open Access Journals (Sweden)

    Agoeng Tjahajani

    2013-05-01

    Full Text Available Previous work showed that 25% of inner layer Aloe vera leaf extract was effective as anti-inflammatory on the oral mucous of Wistar rats. Objective: The aim of this study was to examine the lowest concentration of whole leaf Aloe vera extract that could accelerate healing process of ulceration on rats. Methods: Sixty inbreeding Spraque Dawley rats was exposed to solution of Hydrogen Peroxide 10% during three days to induced inflammation on the labial mucous inferior of rats. During the next three days, on the same area of control group were topically applied solution of Natrium Chloride 0.9%; while in the treatment group were topically applied 6.25%; 12.5%; 25% of whole leaf of Aloe vera extract for 3x5 minutes with 90 minutes intervals. On the fourth day, five rats in each group were sacrificed; and the same treatment was done on others groups. On the sixth day, five rats of each group were sacrificed; and the same treatment was done on the rest group up to seven days. On the eighth days, the rest groups were sacrificed. Microscopic slides were done. Results: Microscopic slides were analyzed under light microscope and scored. Statistical analysis with Mann-Whitney test showed significant differences between control and treatment group (p<0.05. Conclusion: Application of 6.25% whole leaf Aloe vera extract was the lowest and the most effective concentration in accelerating the healing process of oral mucous ulceration on rats.DOI: 10.14693/jdi.v18i1.56

  14. DINSAR EXPERIMENTS FOR SHANGHAI GROUND SUBSIDENCE MONITORING BY USING ALOS PALSAR DATA

    OpenAIRE

    Wu, Jicang; Zhang, Lina; Ban, Baosong; Li, Tao; XU Caijun; Wen, Yamao

    2011-01-01

    The Advanced Land Observing Satellite (ALOS) PALSAR data is used to take two pass interferometry for extraction of ground subsidence in Shanghai China. At first a set of two pass interferometry with baseline less than 1km are taken and corresponding coherence factor are used to indentify persistent scatterers (PS) so as to form a time series of differential phase of every PSs. A linear subsidence model is applied at all PS points and the subsidence rates of all PSs are obtained through a stac...

  15. Design, formulation and evaluation of Aloe vera chewing gum

    National Research Council Canada - National Science Library

    Aslani, Abolfazl; Ghannadi, Alireza; Raddanipour, Razieh

    2015-01-01

    .... The aim of the present study was to design and evaluate the formulation of Aloe vera chewing gum with an appropriate taste and quality with the indications for healing oral wounds, such as lichen...

  16. Immunomodulatory effects of Aloe vera and its fractions on response of macrophages against Candida albicans.

    Science.gov (United States)

    Farahnejad, Zohreh; Ghazanfari, Tooba; Yaraee, Roya

    2011-12-01

    Natural products are important resources in traditional medicine and have been long used for prevention and treatment of many diseases. Medicinal plants have immunomodulatory properties. Aloe is one of the herbal medicines widely used in natural treatment and alternative therapy for various types of diseases. Aloe vera has been shown to modulate the immune response. Macrophages have been shown to play an essential role as the first line of defense against invading pathogen. Candida albicans is a communal and opportunistic pathogen in humans. In this study, we investigated the effect of A. vera extract and its fractions on infected macrophages with C. albicans. Viability of intraperitoneal macrophages was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. Cell viability of infected macrophages was increased by the extract and dose of some isolated fractions dependently. The extract as well as R100, R50, R30, and R10 fractions of A. vera significantly increased cell viability of macrophages in most doses. R5 and F5 fractions showed no significant difference in comparison with control group. Further studies in animal models and human are necessary to clarify the modulatory effects of A. vera on macrophage function. Isolation and purification of A. vera components are also needed to find out the effective molecules.

  17. Aloe vera for Dry Mouth Denture Patients - Palliative Therapy.

    Science.gov (United States)

    Mohsin, Abdul Habeeb Bin; Reddy, S Varalakshmi; Kumar, M Praveen; Samee, Sarah

    2017-06-01

    Xerostomia (dry mouth) is dryness of the mouth which is due to reduced salivary flow. Lack of adequate saliva causes discomfort in denture wearing patients and decreases retention of dentures. The ability of saliva to wet the tissue surface is one of the most important properties for oral comfort and retention of complete denture in dry mouth patients. This study was conducted to evaluate and compare the wetting ability of therapeutic Aloe vera saliva substitute and commercially available Aqwet saliva substitute on heat-polymerized acrylic resin. Contact angle of liquid saliva substitute was considered as an indicator of wettability. Aloe vera liquid (Aloe vera - Group I) and Aqwet saliva substitute (Aqwet - Group II) were compared in terms of their wetting ability. Forty samples of heat-polymerized acrylic resin were fabricated and divided into two groups with 20 samples in each. Advancing, receding contact angles and angle of hysteresis were measured using contact angle goniometer and DSA4 software analysis. Mann-Whitney U test was applied for statistical analysis of the study. The mean advancing angle and receding angle of Group I (Aloe vera) was smaller than Group II (Aqwet). Mean angle of hysteresis of Group I (Aloe vera) was higher than Group II (Aqwet). Mann-Whitney U test revealed that there is no significant difference in contact angles between the two groups. Wetting ability of Group I (Aloe vera) saliva substitute was found to be better compared to Group II (Aqwet) on heat-polymerized acrylic resin.

  18. Aloe vera: Nature's soothing healer to periodontal disease.

    Science.gov (United States)

    Bhat, Geetha; Kudva, Praveen; Dodwad, Vidya

    2011-07-01

    Recent interest and advances in the field of alternative medicine has promoted the use of various herbal and natural products for multiple uses in the field of medicine. Aloe vera is one such product exhibiting multiple benefits and has gained considerable importance in clinical research. This clinical study focuses on Aloe vera and highlights its property when used as a medicament in the periodontal pocket. A total number of 15 subjects were evaluated for clinical parameters like plaque index, gingival index, probing pocket depth at baseline, followed by scaling and root planing (SRP). Test site comprised of SRP followed by intra-pocket placement of Aloe vera gel, which was compared with the control site in which only SRP was done, and clinical parameters were compared between the two sites at one month and three months from baseline. Results exhibited encouraging findings in clinical parameters of the role of Aloe vera gel as a drug for local delivery. We conclude that subgingival administration of Aloe vera gel results in improvement of periodontal condition. Aloe vera gel can be used as a local drug delivery system in periodontal pockets.

  19. Aloe vera: Nature's soothing healer to periodontal disease

    Science.gov (United States)

    Bhat, Geetha; Kudva, Praveen; Dodwad, Vidya

    2011-01-01

    Background: Recent interest and advances in the field of alternative medicine has promoted the use of various herbal and natural products for multiple uses in the field of medicine. Aloe vera is one such product exhibiting multiple benefits and has gained considerable importance in clinical research. This clinical study focuses on Aloe vera and highlights its property when used as a medicament in the periodontal pocket. Materials and Methods: A total number of 15 subjects were evaluated for clinical parameters like plaque index, gingival index, probing pocket depth at baseline, followed by scaling and root planing (SRP). Test site comprised of SRP followed by intra-pocket placement of Aloe vera gel, which was compared with the control site in which only SRP was done, and clinical parameters were compared between the two sites at one month and three months from baseline. Results: Results exhibited encouraging findings in clinical parameters of the role of Aloe vera gel as a drug for local delivery. Conclusion: We conclude that subgingival administration of Aloe vera gel results in improvement of periodontal condition. Aloe vera gel can be used as a local drug delivery system in periodontal pockets. PMID:22028505

  20. Preliminary trial of aloe vera gruel on HIV infection.

    Science.gov (United States)

    Olatunya, Oladele S; Olatunya, Ayomadewa M; Anyabolu, Henry C; Adejuyigbe, Ebun A; Oyelami, Oyeku A

    2012-09-01

    Ten (10) young women diagnosed with human immunodeficiency virus (HIV) infection in the Wesley Guild Hospital Ilesa, a unit of Obafemi Awolowo University Teaching Hospital, Ile Ife, Osun State, Nigeria who did not meet the national criteria for the use of antiretroviral drugs were managed with 30-40 mL of aloe vera (Aloe barbadensis miller) gruel daily. Their CD4 counts, general improvement, and physical well-being (including weight gain) were monitored over a 1-year period. The findings were compared with those of 20 age- matched controls who were on antiretroviral drugs. One (1) patient who reacted badly to antiretroviral drug switched over to aloe vera. The average weight gain among those on aloe vera was 4.7 kg compared to 4.8 kg by those on antiretroviral drug (p=0.916). The average rise in CD4 count among them was 153.7 cells/μL compared to 238.85 cells/μL among the controls (p=0.087). There was no significant side effect(s) in either group except in the 1 patient who switched over from antiretroviral drugs to aloe vera gruel. These preliminary data suggest that consumption of aloe vera may be of help to HIV-infected individuals in the tropics, given its availability and inexpensiveness.

  1. Extracts from Aloe ferox and Withania somnifera inhibit Candida ...

    African Journals Online (AJOL)

    SERVER

    2008-01-04

    Jan 4, 2008 ... Six clinical isolates of N. gonorrhea were procured from the STI unit of the National Health Laboratory Service in Johannesburg. They were maintained on New York City agar in the laboratory. Nine laboratory strains of C. albicans were cultured and maintained on. Sabouraud Dextrose agar. Sensitivity ...

  2. Aloe plant extracts as alternative larvicides for mosquito control

    African Journals Online (AJOL)

    SERVER

    2008-04-03

    Apr 3, 2008 ... carried out in triplicate using 20 larvae for each replicate assay. The larvae were placed in 50 ml disposable plastic cups containing 15 ml of test solution and fed on tetramin fish feed during all testing. Larvae were considered dead if they were unrousable within a period of time, even when gently prodded.

  3. Process Optimization of Pontianak Aloe vera Powder (Aloe chinensis Beakker as Raw Material for Hand body lotion

    Directory of Open Access Journals (Sweden)

    Nana Supriyatna

    2010-12-01

    Full Text Available Process optimization of Pontianak aloe vera powder (Aloe chinensis Beaker  as raw material for hand body lotion have been conducted on laboratory scale with the aim of obtaining the optimum conditions of processing technologies through the addition of maltodextrin and Carboxy Methyl Cellulase. The optimum results achieved at the ratio of maltodextrin to the CMC by 90%: 10%. Characteristics of aloe vera powder produced showing yellowish white color, water content of 8:56%, 5:50 pH, total solid Aloe 25.5%, weight flour ratio and fresh aloe vera leaves 1.5: 100, aloin content of 164.40 ppm, 257.08 ppm malic acid content, 10.32 ppm vitamin E and 1.4 x 105 colonies/g total bacteria. Making hand body lotion of aloe vera powder optimization results show the characteristic white color with a fragrant aroma, acidity (pH 7:07, 10.34 ppm of malic acid, 1.1 x 104 colony/g total bacteria, and quite stable while storage.

  4. Clinical efficacy of liposome-encapsulated Aloe vera on melasma treatment during pregnancy.

    Science.gov (United States)

    Ghafarzadeh, Masoumeh; Eatemadi, Ali

    2017-06-01

    Melasma is a challenging dermatological condition during pregnancy. The aim of this study was to determine the clinical efficacy of a topical liposome-encapsulated Aloe vera preparation, on melasma in pregnant women. In order to enhance the bioavailability of Aloe vera leaf gel extract (AGE), liposomes encapsulating Aloe vera were prepared and examined for their biochemical properties. In this double-blinded, randomized clinical trial, two groups of pregnant women with melasma who were at their second trimester of pregnancy or later were studied. The patients in the experimental group (90) received liposome-encapsulated AGE (in the form of gel), the other 90 patients were placed in the control group (AGE) for 5 weeks. The liposomes were prepared from a soybean lecithin (SLP-WHITE, 1.0 wt 15 %), with a high trapping efficiency for incorporating about 0.5 wt% AGE concentration. The stable liposomes were then prepared from 1.0 wt% of SLP-WHITE, and different concentrations of AGE were prepared by mechanochemical method using a microfluidizer and homogenizer. The liposomes obtained from 0.25 wt% of AGE were confirmed to be small unilamellar vesicles with a diameter Melasma Area Severity Index (MASI) score was used to record melasma severity, and analysis of co-variance (ANCOVA) was used for data analysis. After 5 weeks of melasma treatment in pregnant women, there was 32% improvement in the MASI score in the liposomal-AGE treatment group as compared to 10% improvement in the control group. The prevalence of melisma decreases with an increase in parity, i.e. 39 (43.35%), 34 (37.8%), 15 (16.7%) and 2 (2.2%) for the experimental groups, and 45 (50%), 35 (38.9%), 10 (11.1%) and 0 (0%) for the control group (p = 0.32). There was a significant difference between experimental and control groups in terms of mean MASI score after the trial. There was no significant difference between the groups regarding family history of melasma, occupation, frequency of sunscreen usage and

  5. Progressive bilateral lipoma arborescens of the knee complicated by juvenile spondyloarthropathy: a case report and review of the literature.

    Science.gov (United States)

    Xue, Jing; Alario, Anthony J; Nelson, Scott D; Wu, Huaxiang

    2013-10-01

    To report an unusual case of lipoma aborescens (LA) presented in a patient with treatment-responsive juvenile spondyloarthropathy (JSPA) and to summarize the clinical manifestations, therapy and prognosis of LA by literature review. We report an atypical case of a 17-year-old patient with an initial presentation of juvenile spondyloarthropathy, whose inflammatory condition was improved successfully by traditional anti-rheumatic drugs and an anti-TNF alpha agent but developed progressive swelling of bilateral knees. Lipoma arborescens were diagnosed in each knee by synovial biopsy obtained by arthroscopic surgery. Fifty-one cases of LA have been reported and are reviewed in detail. Clinically, LA could present as monoarthritis or oligoarthritis. The lateral compartment of the knee is the most common site of involvement. Several cases were reported as a comorbidity of inflammatory diseases, but were not improved by anti-inflammatory therapy. Most patients were diagnosed by classic MRI and biopsy findings. The lesions can be managed by open or arthroscopic surgery, but a minority of the cases may have reoccurrence in the same or opposite joint. LA is a very rare lesion of the synovial and bursal tissue with an unknown etiology. It is considered to be a benign proliferation of the synovial fat associated with trauma, degenerative or inflammatory conditions. LA should be considered as a secondary or comorbid condition in inflammatory arthropathies if other joints respond well to intensive therapy and one or more do not. Copyright © 2013 Elsevier Inc. All rights reserved.

  6. Monosaccharide analysis of succulent leaf tissue in Aloe.

    Science.gov (United States)

    Grace, Olwen M; Dzajic, Amra; Jäger, Anna K; Nyberg, Nils T; Önder, Arife; Rønsted, Nina

    2013-09-01

    The succulent leaf mesophyll in Aloe species supports a burgeoning natural products industry, particularly in Africa. Comparative data necessary to prioritise species with economic potential have been lacking. To survey leaf mesophyll monosaccharide composition in the genus Aloe using a predictive phylogenetic approach. Monosaccharide composition was assessed in 31 species, representing the morphological and taxonomic diversity of Aloe sensu stricto. Leaf mesophyll polysaccharides were partially hydrolysed in a trifluoroacetic acid (TFA)-SilA assay. Oximes and trimethylsilyl ether products were detected by GC-MS. Constituent monosaccharides accounting for the greatest variation among species were identified by principal component analysis. Two plant DNA barcoding regions were sequenced in 28 of the sampled species and the resulting maximum likelihood tree was used to evaluate phylogenetic signal in monosaccharide composition throughout the genus. Nineteen peaks (Rt=16.76-23.67 min) were identified in the GC-MS spectra. All samples were dominated by one constituent; glucose was the major monosaccharide in 19 species, mannose in eight species, and xylose in one species (Aloidendron pillansii). Three monosaccharides therefore account for 90% of the variation in leaf mesophyll in Aloe. Species which do not share this typical monosaccharide profile appear to group outside the core Aloe clade in the phylogeny. Preliminary findings suggest that leaf mesophyll monosaccharide composition is conservative in Aloe. Characterisation of within-species variation and quantitative differences between species will be necessary to authenticate leaf mesophyll products, whereas unusual monosaccharide profiles could be diagnostic in some species. The common glucose-mannose-xylose profile identified in commercially important species is shared by many other Aloe species. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

  7. Reduction of Fasting Blood Glucose and Hemoglobin A1c Using Oral Aloe Vera: A Meta-Analysis.

    Science.gov (United States)

    Dick, William R; Fletcher, Emily A; Shah, Sachin A

    2016-06-01

    Diabetes mellitus is a global epidemic and one of the leading causes of morbidity and mortality. Additional medications that are novel, affordable, and efficacious are needed to treat this rampant disease. This meta-analysis was performed to ascertain the effectiveness of oral aloe vera consumption on the reduction of fasting blood glucose (FBG) and hemoglobin A1c (HbA1c). PubMed, CINAHL, Natural Medicines Comprehensive Database, and Natural Standard databases were searched. Studies of aloe vera's effect on FBG, HbA1c, homeostasis model assessment-estimated insulin resistance (HOMA-IR), fasting serum insulin, fructosamine, and oral glucose tolerance test (OGTT) in prediabetic and diabetic populations were examined. After data extraction, the parameters of FBG and HbA1c had appropriate data for meta-analyses. Extracted data were verified and then analyzed by StatsDirect Statistical Software. Reductions of FBG and HbA1c were reported as the weighted mean differences from baseline, calculated by a random-effects model with 95% confidence intervals. Subgroup analyses to determine clinical and statistical heterogeneity were also performed. Publication bias was assessed by using the Egger bias statistic. Nine studies were included in the FBG parameter (n = 283); 5 of these studies included HbA1c data (n = 89). Aloe vera decreased FBG by 46.6 mg/dL (p aloe vera for significantly reducing FBG (46.6 mg/dL) and HbA1c (1.05%). Further clinical studies that are more robust and better controlled are warranted to further explore these findings.

  8. Impact of goat browsing on Aloe ferox in a South African savanna ...

    African Journals Online (AJOL)

    The extent and impact of the utilisation of Aloe ferox by Boer goats during winter in a South African savanna was determined using a plant-based approach. All Aloe plants rooted within the transects were eaten by goats, with small plants utilised more frequently than tall plants. The density of dying and dead Aloe plants was ...

  9. Qualitative improvement of low meat beef burger using Aloe vera.

    Science.gov (United States)

    Soltanizadeh, Nafiseh; Ghiasi-Esfahani, Hossein

    2015-01-01

    Low meat beef burgers have found their niche in the food markets in developing countries because of their lower price. However, these burgers still lack an acceptable quality. This study investigates the effects of different concentrations of Aloe vera on the quality of this food product. For this purpose, beef burgers were produced with 0%, 1%, 3%, and 5% Aloe vera and the changes in their cooking parameters, lipid oxidation, texture, and appeal to consumers over 7days of refrigerated storage were evaluated. Results indicate that Aloe vera contributed to some extent to decreased cooking loss and diameter reduction in the burgers. Increased concentrations of Aloe vera led to improvements in the water absorption and texture of the burgers as well as their lipid stability. However, a concentration level of 3% led to the most acceptability of the product to the panelists. Finally, it was found that Aloe vera acts as a hydrocolloid and improves the quality of burgers. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Formulation and Characterization of Aceclofenac -Aloe vera Transemulgel.

    Science.gov (United States)

    Raju, Y Prasanna; Haritha, K; Satyanarayana, Rao P; Vandana, K R; Bindu, D Thushara; Vinesha, V; Chowdary, V Harini

    2015-01-01

    The present research was aimed to formulate aceclofenac transemulgel using Aloe vera as gel base. The prepared formulations were subjected to physical characterization, in-vitro and in-vivo assessment. Aceclofenac, a hydrophobic potential non steroidal anti inflammatory drug, causes ulceration upon chronic oral administration, could be formulated into transemulgel to enhance therapeutic efficacy and to lower the unwanted side effects. The transemulgel was prepared from aqueous Aloe vera gel and aceclofenac emulsion. The prepared transemulgel was evaluated for its pH, viscosity, drug content, skin irritation, in-vitro diffusion and accelerated stability studies. The prepared aceclofenac-Aloe vera tranemulgel and commercial aceclofenac gel were subjected to pharmacodynamic studies in albino rats of Wistar strain employing carrageenan induced left hind paw edema method to assess the anti-inflammatory effect. The transemulgel showed a pH of 6.78 and viscosity of 18 cps. In-vitro diffusion data revealed better permeation characteristics. Topical application of formulation found no skin irritation. Stability study has proved the integrity of the formulation. The prepared aceclofenac Aloe vera transemulgel showed better in-vitro drug release when compared with the commercial aceclofenac gel formulation. Anti-inflammatory activity in treated rats showed the significant paw volume reduction at pAloe vera as gel base.

  11. Hemlock alkaloids from Socrates to poison aloes.

    Science.gov (United States)

    Reynolds, Tom

    2005-06-01

    Hemlock (Conium maculatum L. Umbelliferae) has long been known as a poisonous plant. Toxicity is due to a group of piperidine alkaloids of which the representative members are coniine and gamma-coniceine. The latter is the more toxic and is the first formed biosynthetically. Its levels in relation to coniine vary widely according to environmental conditions and to provenance of the plants. Surprisingly, these piperidine alkaloids have turned up in quite unrelated species in the monocotyledons as well as the dicotyledons. Aloes, for instance, important medicinal plants, are not regarded as poisonous although some species are very bitter. Nevertheless a small number of mostly local species contain the alkaloids, especially gamma-coniceine and there have been records of human poisoning. The compounds are recognized by their characteristic mousy smell. Both acute and chronic symptoms have been described. The compounds are neurotoxins and death results from respiratory failure, recalling the effects of curare. Chronic non-lethal ingestion by pregnant livestock leads to foetal malformation. Both acute and chronic toxicity are seen with stock in damp meadows and have been recorded as problems especially in North America. The alkaloids derive biosynthetically from acetate units via the polyketide pathway in contrast to other piperidine alkaloids which derive from lysine.

  12. Chemopreventive and antioxidant activity of the chamazulene-rich essential oil obtained from Artemisia arborescens L. growing on the Isle of La Maddalena, Sardinia, Italy.

    Science.gov (United States)

    Ornano, Luigi; Venditti, Alessandro; Ballero, Mauro; Sanna, Cinzia; Quassinti, Luana; Bramucci, Massimo; Lupidi, Giulio; Papa, Fabrizio; Vittori, Sauro; Maggi, Filippo; Bianco, Armanodoriano

    2013-08-01

    The essential oils of Artemisia arborescens growing in Sardinia (Italy), collected during three plant growth stages, i.e., from the vegetative stage to post-blooming time, were characterized. Moreover, the in vitro antiproliferative and antioxidant activities of the oil isolated from aerial parts collected in February were evaluated. The essential oils belonged to the β-thujone/chamazulene chemotype, notably with the highest amount of chamazulene (ca. 52%) ever detected up to now in the genus Artemisia and, in general, in essential oils. Quantitative variations in the oil composition were observed as the plant passes from the vegetative to the blooming stage. The oil was tested for its potential tumor cell growth-inhibitory effect on T98G, MDA-MB 435S, A375, and HCT116 human cell lines, using the MTT (=3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay. The highest activity was observed on A375 and HCT116 cell lines, with IC50 values of 14 μg/ml. Moreover, the in vitro antioxidant and free radical-scavenging assays revealed the oil to be an effective scavenger of the ABTS radical cation, with an activity comparable to that of Trolox(®) . These results support the use of A. arborescens oil for the treatment of inflamed skin conditions. Finally, the composition of the polar fraction of the A. arborescens aerial parts was also examined, and the main component detected was 5-O-caffeoylquinic acid, which was identified for the first time in this plant. Copyright © 2013 Verlag Helvetica Chimica Acta AG, Zürich.

  13. Effect of different drying procedures on the bioactive polysaccharide acemannan from Aloe vera (Aloe barbadensis Miller).

    Science.gov (United States)

    Minjares-Fuentes, Rafael; Rodríguez-González, Víctor Manuel; González-Laredo, Rubén Francisco; Eim, Valeria; González-Centeno, María Reyes; Femenia, Antoni

    2017-07-15

    The main effects of different drying procedures: spray-, industrial freeze-, refractance window- and radiant zone-drying, on acemannan, the main bioactive polysaccharide from Aloe vera gel, were investigated. All the drying procedures caused a considerable decrease in the acemannan yield (∼40%). Degradation affected not only the backbone, as indicated by the important losses of (1→4)-linked mannose units, but also the side-chains formed by galactose. In addition, methylation analysis suggested the deacetylation of mannose units (>60%), which was confirmed by 1H NMR analysis. Interestingly, all these changes were reflected in the functional properties which were severely affected. Thus, water retention capacity values from processed samples decreased ∼50%, and a reduction greater than 80% was determined in swelling and fat adsorption capacity values. Therefore, these important modifications should be taken into consideration, since not only the functionality but also the physiological effects attributed to many Aloe vera-based products could also be affected. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Influence of Polysaccharides from Aloe vera (Aloe barbadensis Miller, Liliaceae on Mechanically Induced Cough in Cats

    Directory of Open Access Journals (Sweden)

    Martina Šutovská

    2010-01-01

    Full Text Available Different plants have been reported to be a source of polysaccharides that possess a great variety of biological activities, including cough suppression. The experiments were aimed on the influence of two polysaccharides isolated from the gel (A, HF1-Z and one from the epidermis (SL-1 of Aloe vera leaves, characterized by chemical composition and structural properties, on the cough reflex. The method of mechanically induced cough was used in conscious cats. A nylon fibre directed individually into the mucous area of the laryngopharyngeal and tracheobronchial airways via endotracheal cannula elicited the cough reflex. Statistical evaluation registered cough reflex indicators (number of cough efforts - NE, cough frequency - NE min–1 intensity of cough attacks during expiration and inspiration - IA+ and IA-, intensity of maximal inspiratory and expiratory cough efforts - IME- and IME+ showed that gel polysaccharide HF1-Z possessed higher cough-suppressing activity than dropropizine, a nonnarcotic peripheral cough suppressant. Their antitussive activity was accompanied mainly with a decrease of NE, IA+ and IA- from both laryngopharyngeal and tracheobronchial mucous areas of the airways. Results of the presented study also showed minimal influence of polysaccharide HF1-Z on expectoration predictor, IME- and IME+. New pharmacodynamic property of polysaccharides isolated from Aloe vera was shown during experiments, which could be a base of their possible therapeutic use in this indication in future.

  15. Modulation of Immune Response of BALB/Mice Bearing Lymphoma L5178Y Treated with Bitter Yellow Juice of Aloe vera (L) in vivo.

    Science.gov (United States)

    Oronzo-Barocio, Arturo; Zaitseva, Galina; Chavez-Anaya, Azucena; Arceta-Gonzalez, Veronika I.; Puebla-Perez, Ana Marija; Alfaro-Bustamante, Fernando; Zimina, Irina V.; Arion, Vitaly Ya.

    1999-04-01

    Aloe vera (L), a plant of African origin, has been introduced in Mexico since XVIth century. It has been used in the treatment of many diseases of immune system. In the present study we investigated a specific and non-specific immune response of BALB/c mice, healthy and immunosuppressed with murine lymphoma L5178Y, treated with bitter yellow juice (extract) of Aloe vera (L). We observed that the immunosuppressed mice, treated with the whole extract of the bitter yellow juice achieved restoration of immunological parameters in cellular immune response and phagocytosis. On the other hand, the humoral immunity was not restored. Also, in the healthy rodents treated with the extract, it caused the stimulation of specific and non-specific responses, the results had significant differences with the obtained ones in untreated mice.

  16. Arquitetura, anatomia e histoquímica das folhas de Gomphrena arborescens L.f. (Amaranthaceae Architecture, anatomy and histochemistry of the leaves of Gomphrena arborescens L.f. (Amaranthaceae

    Directory of Open Access Journals (Sweden)

    Suzane Margaret Fank-de-Carvalho

    2005-06-01

    Full Text Available As folhas de Gomphrena arborescens L.f., nativa do Cerrado, são utilizadas contra dismenorréia na medicina popular. As investigações morfológica, anatômica e histoquímica das folhas visam fornecer elementos para taxonomia, para controle de qualidade de drogas e identificação microscópica de fitoterápicos. Folhas foram coletadas em Brasília, DF, nas áreas de Cerrado do Centro Olímpico da Univesidade de Brasília e na Reserva Ecológica do IBGE. Foram efetuadas análises morfológicas, anatômicas e histoquímicas. Os estudos de arquitetura foliar, densidade estomática e de vênulas terminais foram efetuados em folhas diafanizadas, apresentando médias de 42,39±15,50 estômatos mm-2 e 11,7±3,55 vênulas terminais mm². As folhas apresentaram duas formas de tricomas tectores. O mesofilo bilateral apresenta tecido clorenquimático disposto radialmente à bainha parenquimática completa que envolve os feixes vasculares, características da síndrome Kranz associada ao ciclo fotossintético C4. O amido concentra-se na bainha parenquimática. As folhas são ricas em celulose, drusas de oxalato de cálcio, proteínas, lignina e outros compostos fenólicos, entre eles os flavonóides. As folhas apresentam morfologia variável, mas o padrão anatômico e de venação são constantes.Leaves of the Brazilian Cerrado plant Gomphrena arborescens L.f. are used against dismenorea in popular medicine. The morphological, anatomical and histochemical investigation aims to help taxonomy, quality control of drugs and microscopical identification of phytotherapics. The leaves have been collected in Brasília, DF, in the Olympical Center of the University of Brasilia and in the Ecological Reserve of IBGE. The leaf architecture, stomatal and veinlet densities have been studied in clarified leaves, presenting average of 42,39±15,50 stomata mm² and 11,7±3,55 veinlet mm-2. Leaves presented two forms of tector trichomes. The bilateral mesophyll

  17. EXTRACT

    DEFF Research Database (Denmark)

    Pafilis, Evangelos; Buttigieg, Pier Luigi; Ferrell, Barbra

    2016-01-01

    The microbial and molecular ecology research communities have made substantial progress on developing standards for annotating samples with environment metadata. However, sample manual annotation is a highly labor intensive process and requires familiarity with the terminologies used. We have the...... and text-mining-assisted curation revealed that EXTRACT speeds up annotation by 15-25% and helps curators to detect terms that would otherwise have been missed.Database URL: https://extract.hcmr.gr/......., organism, tissue and disease terms. The evaluators in the BioCreative V Interactive Annotation Task found the system to be intuitive, useful, well documented and sufficiently accurate to be helpful in spotting relevant text passages and extracting organism and environment terms. Comparison of fully manual...

  18. Effect of vehicles on topical application of aloe vera and arnica montana components.

    Science.gov (United States)

    Bergamante, Valentina; Ceschel, Gian Carlo; Marazzita, Sergio; Ronchi, Celestino; Fini, Adamo

    2007-10-01

    In this study two types of gels and microemulsions are investigated for their ability to dissolve, release, and induce the permeation of helenalin, a flavonoid responsible for the anti-inflammatory activity of arnica montana extract, and aloin, an anthrone-C-glucosyls with antibacterial activity present in aloe vera extract. The release of these agents from each vehicle was followed by HPLC, and transcutaneous permeation was examined using a modified Franz cell and a porcine skin membrane. The study showed that a microemulsion can be a good vehicle to increase the permeation of helenalin, while the gel formulation, containing Sepigel 305, proved able to reduce the release and permeation of aloin, with a consequent activity limited to the surface of application, without any permeation. This is in accordance with the necessity to avoid this process, since human skin fibroblasts can metabolize absorbed aloin into a structurally related compound that increases the sensitivity of skin to ultraviolet light.

  19. FTIR SPECTRUM COMPARISON OF PLANTS purgatives [ ALOE VERA LINNEO . ALOE BARBADENSIS MILLER , RHEUM PALMATUM ( Rhubarb ) , RHAMNUS Pursiana ( SACRED SHELL ) AND CASSIA SP. (SEN )

    OpenAIRE

    Saavedra N., F.; Laboratorio de Química Básica y Aplicada. Laboratorio de Bioquímica, Facultad de Farmacia y Bioquímica,Universidad Nacional Mayor de San Marcos,Lima,Perú.; Ale B., N.; Laboratorio de Química Analítica e Instrumentación, Facultad de Química e Ingeniería Química,Universidad Nacional Mayor de san Marcos,Lima,Perú.; Gordillo R., G.; Laboratorio de Química Básica y Aplicada, Laboratorio de Bioquímica, Facultad de Farmacia y Bioquímica,Universidad Nacional Mayor de San Marcos,Lima,Perú.; Rodríguez B., M.; Facultad de Química e Ingeniería Química,Universidad Nacional Mayor de San Marcos,Lima,Perú.; Apesteguía I., J.; Laboratorio de Química Básica y Aplicada, Facultad de Farmacia y Bioquímica,Universidad Nacional Mayor de San Marcos,Lima,Perú.; Jurado T., B.; Laboratorio de Química Básica y Aplicada, Facultad de Farmacia y Bioquímica,Universidad Nacional Mayor de San Marcos,Lima,Perú.

    2014-01-01

    FTIR identified the carbonyl C = O functional group of the anthraquinone derivatives in aqueous macerated and alcoholics purges as aloe plants (Aloe vera L. , Aloe barbadensis Miller ) , senna ( Cassia spp . ) , Rhubarb ( Rheum palmatum) and shell sagrada (Rhamnus Pursiana ) without prior separation of the components by chromatographic methods or other physical and chemical methods . We show that the absorption maxima for the C = O carbonyl group of the anthraquinone derivatives in aqueous an...

  20. Studies on the Use of Plant Extracts for the Prevention of Bacterial ...

    African Journals Online (AJOL)

    The various extracts and standard drugs reduced bacterial attachment on the catheters in the order: ciprofloxacin > Psidium guajava > Aloe vera > gentamicin > Gongronema latifolium > untreated sections. Carica papaya, Ocimum gratisimum and Vernonia amygdalina potentiated bacterial attachment. Furthermore, the ...

  1. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study

    Science.gov (United States)

    Bazvand, Leila; Aminozarbian, Mohammad Ghasem; Farhad, Alireza; Noormohammadi, Hamid; Hasheminia, Seyed Mohsen; Mobasherizadeh, Sina

    2014-01-01

    Background: The aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, Propolis and Aloe vera on Enterococcus faecalis in deep dentin. Materials and Methods: Ninety fresh extracted single-rooted teeth were used in a dentin block model. Seventy-five teeth were infected with E. faecalis and divided into four experimental groups (n = 15). Experimental groups were treated with triantibiotic mixture with distilled water, 0.2% chlorhexidine gel, 70% ethanol + Propolis and Aloe vera. Fifteen teeth treated with distilled water as the positive control and 15 samples, free of bacterial contamination, were considered as the negative control. Gates-Glidden drill #4 was used for removal of surface dentin and Gates-Glidden drill #5 was used to collect samples of deep dentin. The samples were prepared and colony-forming units were counted. Data were analyzed by one-way ANOVA and post hoc Tukey tests. Statistical significance was defined at P 0.05). Aloe vera had antibacterial effects on E. faecalis, but in comparison with other medicaments, it was less effective (P < 0.05). Conclusion: This experimental study showed that triantibiotic mixture, 0.2% chlorhexidine gel, Propolis and Aleo vera were relatively effective against E. faecalis. All the intracanal medicements had similar effects on E. faecalis in deep dentin except for Aloe vera. PMID:25225560

  2. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study

    Directory of Open Access Journals (Sweden)

    Leila Bazvand

    2014-01-01

    Full Text Available Background: The aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, Propolis and Aloe vera on Enterococcus faecalis in deep dentin. Materials and Methods: Ninety fresh extracted single-rooted teeth were used in a dentin block model. Seventy-five teeth were infected with E. faecalis and divided into four experimental groups (n = 15. Experimental groups were treated with triantibiotic mixture with distilled water, 0.2% chlorhexidine gel, 70% ethanol + Propolis and Aloe vera. Fifteen teeth treated with distilled water as the positive control and 15 samples, free of bacterial contamination, were considered as the negative control. Gates-Glidden drill #4 was used for removal of surface dentin and Gates-Glidden drill #5 was used to collect samples of deep dentin. The samples were prepared and colony-forming units were counted. Data were analyzed by one-way ANOVA and post hoc Tukey tests. Statistical significance was defined at P 0.05. Aloe vera had antibacterial effects on E. faecalis, but in comparison with other medicaments, it was less effective (P < 0.05. Conclusion: This experimental study showed that triantibiotic mixture, 0.2% chlorhexidine gel, Propolis and Aleo vera were relatively effective against E. faecalis. All the intracanal medicements had similar effects on E. faecalis in deep dentin except for Aloe vera.

  3. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study.

    Science.gov (United States)

    Bazvand, Leila; Aminozarbian, Mohammad Ghasem; Farhad, Alireza; Noormohammadi, Hamid; Hasheminia, Seyed Mohsen; Mobasherizadeh, Sina

    2014-07-01

    The aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, Propolis and Aloe vera on Enterococcus faecalis in deep dentin. Ninety fresh extracted single-rooted teeth were used in a dentin block model. Seventy-five teeth were infected with E. faecalis and divided into four experimental groups (n = 15). Experimental groups were treated with triantibiotic mixture with distilled water, 0.2% chlorhexidine gel, 70% ethanol + Propolis and Aloe vera. Fifteen teeth treated with distilled water as the positive control and 15 samples, free of bacterial contamination, were considered as the negative control. Gates-Glidden drill #4 was used for removal of surface dentin and Gates-Glidden drill #5 was used to collect samples of deep dentin. The samples were prepared and colony-forming units were counted. Data were analyzed by one-way ANOVA and post hoc Tukey tests. Statistical significance was defined at P 0.05). Aloe vera had antibacterial effects on E. faecalis, but in comparison with other medicaments, it was less effective (P Aloe vera.

  4. Efficacy of orally administered powdered aloe juice (Aloe ferox) against ticks on cattle and ticks and fleas on dogs

    OpenAIRE

    Fourie, J.J.; L.J. Fourie; I.G. Horak

    2005-01-01

    The efficacy of orally administered powdered aloe juice (Aloe ferox) was evaluated against ticks on cattle and against ticks and fleas on dogs. Twelve calves were each infested over a 25-day period with approximately 4000 larvae of Rhipicephalus (Boophilus) decoloratus and allocated to 3 groups of 4 calves each. Three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg / kg body weight and those in another 25 mg / kg body weight of powd...

  5. Glacier velocity Changes at Novaya Zemlya revealed by ALOS1 and ALOS2

    Science.gov (United States)

    Konuma, Y.; Furuya, M.

    2016-12-01

    Matsuo and Heki (2013) revealed substantial ice-mass loss at Novaya Zemlya by Gravity Recovery And Climate Experiment (GRACE). In addition, the elevation thinning (Moholdt et al., 2012) and glacier retreat (Carr et al., 2014) has been reported. Melkonian et al. (2016) showed velocities map at coastal area of Novaya Zemlya by using Worldview, Landsat, ASTER and TerraSAR-X images. However, the entire distributions of ice speed and the temporal evolution remain unclear. In this study, we measured the glacier velocities using L-band SAR sensor onboard ALOS1 and ALOS2. We analyzed the data using pixel-offset tracking technique. We could observe the entire glaciated region in 2007-2008 winter and 2008-2009 winter. In particular, we could examine the velocities at middle of the glaciated region from 2006 to 2015 due to the availability of high-temporal resolution SAR data. As a result, we found the most glaciers in Novaya Zemlya have been accelerating since 1990s (Strozzi et al., 2008). Specially, Shokalskogo glacier has dramatically accelerated from the maximum of 300 ma-1 in 1998 to maximum of 600 ma-1 in 2015. Additionally, it turns out that there are marked differences in the glacier's velocities between the Barents Sea side and the Kara Sea side. The averaged maximum speed of the glaciers in Barents Sea side were approximately two times faster than that in Kara Sea side. We speculate the causes as the difference of topography under the calving front and sea-ice concentration. While each side has many calving glaciers, the fjord distribution in the Barents Sea side is much broader than in the Kara Sea side. Moreover, sea-ice concentration in the Barents Sea is lower than the Kara Sea, which might affect the glaciers' speed distribution.

  6. Distribution, diversity and conservation of the genus Aloe in Kenya ...

    African Journals Online (AJOL)

    The diversity patterns indicate a high concentration of taxa in three areas that are identified as Aloe hot spots and thus of high priority for conservation of the genus: The Kulal–Nyiro–Ndotos–Marsabit area in the north, the Taita-Shimba Hills zone to the southeast and the Naivasha–Baringo area in the Rift Valley. Most of the ...

  7. ALOS PALSAR and UAVSAR Soil Moisture in Field Campaigns

    Science.gov (United States)

    As part of our ongoing analysis of L-band radar mapping of soil moisture we are evaluating the role that ALOS PALSAR data can play in the development of radar retrieval algorithms for the future NASA Soil Moisture Active Passive (SMAP) satellite. Differences in configurations must be assessed to det...

  8. THE BOTANY AND CHEMSTRY OF ALOES OF AFRICA

    African Journals Online (AJOL)

    botany of many members of this genus and consequently also spurred taxonomic research of the taxa in specific geographic regions. Recent taxonomic studies on the genus Aloe in eastern and northeastern Africa indicated the presence of a large number of endemic species. For example in eastern Africa, of the 83 species ...

  9. Uso de la jalea de aloe en la cervicitis aguda

    Directory of Open Access Journals (Sweden)

    Miguel Lugones Botell

    1999-04-01

    Full Text Available Se realiza un ensayo clínico a doble ciegas en un grupo de pacientes con cervicitis aguda, las cuales fueron atendidas en los policlínicos "Jorge Ruíz Ramírez" y "26 de Julio", del municipio Playa. Se analizaron diferentes síntomas y signos así como la respuesta al tratamiento con curas de aloe. Se concluye que el aloe es efectivo en el tratamiento de esta entidad y que se incrementan sus resultados favorables cuando se asocia con ácido lácticoA double-blind clinical trial was conducted in a group of patients with acute cervicitis that received attention at the"Jorge Ruiz Ramírez" and"26 de Julio" polyclinics, in the municipality of Playa. Different symptoms and signs as well as the response to aloe cures were analyzed. It was concluded that aloe is effective to treat this disease and that its favorable results increase when it is associated with lactic acid

  10. Chemical composition of volatile constituents from the leaves of Aloe ...

    African Journals Online (AJOL)

    Volatile compounds oils play a significant role in perfumery, cosmetic, medicinal and pharmaceutical industries. In our protracted research for new useful essential oils, a volatile oil from Aloe ferox was obtained by prolonged hydrodistillation. This volatile oil was subjected to GC/MS analysis to identify the major constituents ...

  11. Chemical composition of volatile constituents from the leaves of Aloe ...

    African Journals Online (AJOL)

    AJB SERVER

    2006-09-18

    Sep 18, 2006 ... Volatile compounds oils play a significant role in perfumery, cosmetic, medicinal and pharmaceutical industries. In our protracted research for new useful essential oils, a volatile oil from Aloe ferox was obtained by prolonged hydrodistillation. This volatile oil was subjected to GC/MS analysis to identify.

  12. Comparative antimicrobial activities of aloe vera gel and leaf

    African Journals Online (AJOL)

    SERVER

    2005-10-14

    Oct 14, 2005 ... 1Department of Microbiology, Federal University of Technology, Akure, Nigeria. 2Department of ... ingredient to retain the water in the leaf. This is called the .... Health. WorldOnline. . Grindlay D, Reynadds T (1986) “The Aloe vera Phenomenon. A review.

  13. Preliminary Antiplaque Efficacy of Aloe Vera Mouthwash on 4 Day ...

    African Journals Online (AJOL)

    Background: Due to increasing resistance to antibiotics and rising incidence of oral diseases, there is a need for alternative treatment modalities to combat oral diseases. The aim of the present study was to access the effect of Aloe vera mouthwash on the dental plaque in the experimental period of 4 days and to compare it ...

  14. Fumaric acid, an antibacterial component of Aloe vera L. | He ...

    African Journals Online (AJOL)

    The acetoacetate fraction partitioned from Aloe vera L. showed an effective antibacterial activity against four clinically frequent pathogenic bacteria. Through a series of chromatographic methods and activity assays, one compound was obtained and it has potent antibacterial activity. Based on the data of mass spectrometry, ...

  15. Insecticidal and insect productivity reduction capacities of aloe vera ...

    African Journals Online (AJOL)

    Two important medicinal plants, Aloe vera and Bryophyllum pinnatum were admixed with maize grains (Popcorn) at dosages of 1g, 2g and 5g treatments per 50g of maize. Results showed that both lower and higher dosages had toxic effects on Tribolium castaneum but the best results were obtained in the grains treated ...

  16. Survey of chemical manure on morphological traits in Iranian Aloe ...

    African Journals Online (AJOL)

    This experiment was conducted at the Institute of National Genetic Engineering and Biotechnology in Iran to evaluate the effect of different amount of fertilizers on the leaf and plant characteristics, as well as the yield characteristics of Aloe vera. There were 6 different treatments viz., T1 = 100% soil (control), T2 = 100 PK ...

  17. Screening of Polyalthia longifolia and Aloe vera for their ...

    African Journals Online (AJOL)

    The work focuses on the screening of Polyalthia longifolia and Aloe vera for their phytoextractability of heavy metal in soil of the Niger Delta. 5kg of soil was polluted with 100 mg of Zn, Fe and Pb each replicated 9 times. Each set was separated into 3 subgroups. The subgroups were phytoextracted with Polyalthia longifolia ...

  18. Accuracy assessment of ALOS optical instruments: PRISM and AVNIR-2

    Science.gov (United States)

    Tadono, Takeo; Shimada, Masanobu; Iwata, Takanori; Takaku, Junichi; Kawamoto, Sachi

    2017-11-01

    This paper describes the updated results of calibration and validation to assess the accuracies for optical instruments onboard the Advanced Land Observing Satellite (ALOS, nicknamed "Daichi"), which was successfully launched on January 24th, 2006 and it is continuously operating very well. ALOS has an L-band Synthetic Aperture Radar called PALSAR and two optical instruments i.e. the Panchromatic Remotesensing Instrument for Stereo Mapping (PRISM) and the Advanced Visible and Near Infrared Radiometer type-2 (AVNIR-2). PRISM consists of three radiometers and is used to derive a digital surface model (DSM) with high spatial resolution that is an objective of the ALOS mission. Therefore, geometric calibration is important in generating a precise DSM with stereo pair images of PRISM. AVNIR-2 has four radiometric bands from blue to near infrared and uses for regional environment and disaster monitoring etc. The radiometric calibration and image quality evaluation are also important for AVNIR-2 as well as PRISM. This paper describes updated results of geometric calibration including geolocation determination accuracy evaluations of PRISM and AVNIR-2, image quality evaluation of PRISM, and validation of generated PRISM DSM. These works will be done during the ALOS mission life as an operational calibration to keep absolute accuracies of the standard products.

  19. Karyotypes in Ethiopian Aloe species (Xanthorrhoeaceae: Asphodeloideae)

    DEFF Research Database (Denmark)

    Fentaw, Eshetu; Dagne, Kifle; Rønsted, Nina

    2013-01-01

    A cytogenetic survey of 18 species in the succulent-leaved genus Aloe L. (Xanthorrhoeaceae subfamily Asphodeloideae) in Ethiopia was undertaken towards a more complete genetic characterisation of the genus throughout its distribution in Africa, Arabia and Madagascar. Somatic metaphase chromosomes...

  20. Separation of Water to Concentrate Aloe Vera Juice

    National Research Council Canada - National Science Library

    Abdullah, Ishfaq; uzzaman Malik, Waheed; Saleemi, Hassan; Bilal, Ahmad; Shahid, M. Rizwan

    2014-01-01

    Aloe Vera is a succulent plant and is found in different arid areas. Due to its healing effect and soothing properties it is being used in herbal medicines for years and its importance has grown due to use in cosmetic products...

  1. Antimalarial Anthrone and Chromone from the Leaf Latex of Aloe ...

    African Journals Online (AJOL)

    In Ethiopian traditional medicine, the leaf latex of Aloe debranan Chrstian is used for the treatment of several diseases including malaria. In an ongoing search for effective, safe and cheap antimalarial agents from plants, the leaf latex of A. debrana was tested for its in vivo antimalarial activity, in a 4-day suppressive assay ...

  2. Aloe; Beyond use as cosmetics | Pili | Dar Es Salaam Medical ...

    African Journals Online (AJOL)

    Dar Es Salaam Medical Students' Journal. Journal Home · ABOUT · Advanced Search · Current Issue · Archives · Journal Home > Vol 15, No 1 (2008) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. Aloe; Beyond use as cosmetics. K Pili. Abstract. No Abstract ...

  3. Isolation of volatile compounds of Aloe excelsa (Berger ...

    African Journals Online (AJOL)

    Industrial and pharmacological applications of volatile and non-volatile compounds isolated from plants have been dominating the commercial sector over the recent two decades. Attempts in isolation of volatile compounds of aloes have impact on the medicinal as well as the cosmetic industries. Volatile compound isolation ...

  4. Quantitative Analysis of Aloins and Aloin-Emodin in Aloe Vera Raw Materials and Finished Products Using High-Performance Liquid Chromatography: Single-Laboratory Validation, First Action 2016.09.

    Science.gov (United States)

    Kline, David; Ritruthai, Vicha; Babajanian, Silva; Gao, Quanyin; Ingle, Prashant; Chang, Peter; Swanson, Gary

    2017-05-01

    A single-laboratory validation study is described for a method of quantitative analysis of aloins (aloins A and B) and aloe-emodin in aloe vera raw materials and finished products. This method used HPLC coupled with UV detection at 380 nm for the aloins and 430 nm for aloe-emodin. The advantage of this test method is that the target analytes are concentrated from the sample matrix (either liquid or solid form) using stepwise liquid-liquid extraction (water-ethyl acetate-methanol), followed by solvent evaporation and reconstitution. This sample preparation process is suitable for different forms of products. The concentrating step for aloins and aloe-emodin has enhanced the method quantitation level to 20 parts per billion (ppb). Reversed-phase chromatography using a 250 × 4.6 mm column under gradient elution conditions was used. Mobile phase A is 0.1% acetic acid in water and mobile phase B is 0.1% acetic acid in acetonitrile. The HPLC run starts with a 20% mobile phase B that reaches 35% at 13 min. From 13 to 30 min, mobile phase B is increased from 35 to 100%. From 30 to 40 min, mobile phase B is changed from 100% back to the initial condition of 20% for re-equilibration. The flow rate is 1 mL/min, with a 100 μL injection volume. Baseline separation (Rs > 2.0) for aloins A and B and aloe-emodin was observed under this chromatographic condition. This test method was validated with raw materials of aloe vera 5× (liquid) and aloe vera 200× (powder) and finished products of aloe concentrate (liquid) and aloe (powder). The linearity of the method was studied from 10 to 500 ppb for aloins A and B and aloe-emodin, with correlation coefficients of 0.999964, 0.999957, and 0.999980, respectively. The test method was proven to be specific, precise, accurate, rugged, and suitable for the intended quantitative analysis of aloins and aloe-emodin in raw materials and finished products. The S/N for aloins A and B and aloe-emodin at 10 ppb level were 12, 10, and 8

  5. Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: Optical properties

    Science.gov (United States)

    Gunalan, Sangeetha; Sivaraj, Rajeshwari; Venckatesh, Rajendran

    2012-11-01

    In this paper, we report on the synthesis of nanostructured copper oxide particles by both chemical and biological method. A facile and efficient synthesis of copper oxide nanoparticles was carried out with controlled surface properties via green chemistry approach. The CuO nanoparticles synthesized are monodisperse and versatile and were characterized with the help of UV-Vis, PL, FT-IR, XRD, SEM, and TEM techniques. The particles are crystalline in nature and average sizes were between 15 and 30 nm. The morphology of the nanoparticles can be controlled by tuning the amount of Aloe vera extract. This new eco-friendly approach of synthesis is a novel, cheap, and convenient technique suitable for large scale commercial production and health related applications of CuO nanoparticles.

  6. Effect of Azadirachta indica (Neem) and Aloe vera as compared to subantimicrobial dose doxycycline on matrix metalloproteinases (MMP)-2 and MMP-9: An in-vitro study

    Science.gov (United States)

    Kudalkar, Mithun D.; Nayak, Aarati; Bhat, Kishore S.; Nayak, Ranganath N.

    2014-01-01

    Background: A critical outcome of periodontal diseases is degradation of collagen in the periodontal tissues, by enzymes such as Matrix Metallo-Proteinases (MMPs). Doxycycline is known to down-regulate the activity of MMPs. Azadirachta indica (Neem) and Aloe vera are herbs known to have an anti-inflammatory effect. The present study was designed to evaluate the anti-inflammatory effect of Neem and Aloe vera by way of its inhibitory effect on MMP-2 and MMP-9 activity in cases of chronic periodontitis and compare it with doxcycline. Materials and Methods: A total of 30 subjects were enrolled in this study. Gingival tissue samples were obtained from patients diagnosed with the chronic periodontitis. The tissue extracts were treated with the said drug solutions and inhibition of MMP-2 and MMP-9 was analyzed. Enzymatic activity was detected by electrophoresis. The data was subjected to Student's paired t-test. Results: The results showed that the activity of MMP-2 and MMP-9 was significantly decreased by the use of doxycycline, Neem and Aloe vera. A 53.5% reduction in the MMP-2 and 52.5% reduction in the MMP-9 activity was seen when samples were subjected to Neem treatment at the concentration of 1500 μg/ml. Tissues treated with Aloe vera in the concentration of 2000 μg/ml showed a 20.09% reduction in the MMP-2 and 20.4% reduction in the MMP-9 activity. Doxycycline in the concentration of 300 μg/ml, showed an 82.1% reduction in the MMP-2 and 82.6% reduction in the MMP-9 activity. Conclusion: The present study demonstrated an inhibitory effect of Neem and Aloe vera on MMP-2 and MMP-9, which are involved in the extracellular matrix degradation during periodontitis. PMID:25364206

  7. Growth Period Effects on the Protective Properties of Aloe vera Against t-BHP-Induced Oxidative Stress in Chang Cells.

    Science.gov (United States)

    Hwang, Jin-Woo; Kim, Eun-Kyung; Kim, Yon-Suk; Lee, Jae Woong; Lee, Jeong-Jun; Pyo, Han-Jong; Moon, Sang-Ho; Jeon, Byong-Tae; Park, Pyo-Jam

    2015-12-28

    Aloe vera has been used in traditional medicine for the therapy of a variety of disorders, such as wounds and burns. However, few studies have examined the antioxidant capacities of A. vera plants during different growth periods. In order to investigate the effects of growth on antioxidant activity, A. vera was prepared from 2-, 4-, 6-, 8-, and 12-month-old aloe. The extracts from 6-month-old A. vera showed the highest contents of flavonoids (9.750 mg catechin equivalent/g extract) and polyphenols (23.375 mg gallic acid equivalent/g extract) and the highest ferric reducing antioxidant power (0.047 mM ferrous sulfate equivalent/mg extract). The extract from 6-month-old A. vera exhibited the highest free radical scavenging potential, and the lowest IC50 values were found for 2,2-diphenyl-1-picrylhydrazyl (0.26 mg/ml) and alkyl radicals (0.50 mg/ml). In addition, the extract from 6-month-old A. vera showed the greatest effects on cell viability in normal liver cells. Based on these findings, the extract from 6-month-old A. vera was examined further in order to determine its protective potential against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress. The extract from 6-monthold A. vera at a concentration of 0.25 mg/ml showed the highest protective activity against t-BHP-induced reactive oxygen species production. These findings suggested that harvesting regimens were critical in the regulation of effects of the bioactive potential of A. vera on antioxidant activity.

  8. Efficacy of orally administered powdered aloe juice (Aloe ferox against ticks on cattle and ticks and fleas on dogs

    Directory of Open Access Journals (Sweden)

    J.J. Fourie

    2005-06-01

    Full Text Available The efficacy of orally administered powdered aloe juice (Aloe ferox was evaluated against ticks on cattle and against ticks and fleas on dogs. Twelve calves were each infested over a 25-day period with approximately 4000 larvae of Rhipicephalus (Boophilus decoloratus and allocated to 3 groups of 4 calves each. Three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg / kg body weight and those in another 25 mg / kg body weight of powdered aloe juice incorporated in game maintenance pellets, while the animals in the 3rd group received only pellets. Detached female ticks were collected daily and counted and the weights and the fertility of groups of 50 engorged female ticks collected from the animals were ascertained. The powdered aloe juice in the game maintenance pellets had no effect on the tick burdens of the calves or on the fertility of the ticks. Six dogs, in each of 2 groups, were treated daily for 15 consecutive days, commencing on Day -5 before the 1st tick infestation, with either 0.39 g or 0.74 g of powdered aloe juice, administered orally in gelatin capsules, while a 3rd group of 6 dogs served as untreated controls. All the dogs were challenged with Haemaphysalis leachi on Days 0 and +7, and with Ctenocephalides felis on Days+1and +8, and efficacy assessments were made 1 day after flea and 2 days after tick challenge, respectively. Treatment was not effective against ticks or fleas on the dogs.

  9. Efficacy of orally administered powdered aloe juice (Aloe ferox) against ticks on cattle and ticks and fleas on dogs.

    Science.gov (United States)

    Fourie, J J; Fourie, L J; Horak, I G

    2005-12-01

    The efficacy of orally administered powdered aloe juice (Aloe ferox) was evaluated against ticks on cattle and against ticks and fleas on dogs. Twelve calves were each infested over a 25-day period with approximately 4000 larvae of Rhipicephalus (Boophilus) decoloratus and allocated to 3 groups of 4 calves each. Three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg/kg body weight and those in another 25 mg/kg body weight of powdered aloe juice incorporated in game maintenance pellets, while the animals in the 3rd group received only pellets. Detached female ticks were collected daily and counted and the weights and the fertility of groups of 50 engorged female ticks collected from the animals were ascertained. The powdered aloe juice in the game maintenance pellets had no effect on the tick burdens of the calves or on the fertility of the ticks. Six dogs, in each of 2 groups, were treated daily for 15 consecutive days, commencing on Day -5 before the 1st tick infestation, with either 0.39 g or 0.74 g of powdered aloe juice, administered orally in gelatin capsules, while a 3rd group of 6 dogs served as untreated controls. All the dogs were challenged with Haemaphysalis leachi on Days 0 and + 7, and with Ctenocephalides felis on Days + 1 and + 8, and efficacy assessments were made 1 day after flea and 2 days after tick challenge, respectively. Treatment was not effective against ticks or fleas on the dogs.

  10. SINTESIS MEMBRAN NATA ALOE VERA-ETILENDIAMIN DAN KARAKTERISASINYA

    Directory of Open Access Journals (Sweden)

    EB Susatyo

    2014-06-01

    Full Text Available Serat yang terkandung di dalam nata Aloe vera adalah selulosa sehingga dapat digunakan dalam sintesis membran. Telah dilakukan penelitian tentang sintesis membran nata Aloe vera-etilendiamin (nata-en menggunakan sistem vakum cair. Proses preparasi membran berlangsung melalui tiga tahap, yaitu preparasi nata Aloe vera, aktivasi menggunakan asam sulfat, dan modifikasi dengan menggunakan etilendiamin. Tujuan penelitian adalah untuk mempelajari teknik dalam sintesis membran nata-en kemudian melakukan karakterisasi untuk mengetahui karakter strukturnya. Hasil penelitian menunjukkan perilaku yang berbeda dalam hal sifat mekanik dan strukturnya. Membran nata murni memiliki sifat mekanik yang kuat, nata teraktivasi cenderung rapuh, sedangkan nata-en bersifat liat. Spektra infra merah dari ketiga tipe membran (nata murni, nata teraktivasi, dan nata-en secara umum tidak mengalami perubahan yang signifikan, hanya terjadi pergeseran panjang gelombang dari masing-masing membran. Berdasarkan spektra infra merah dapat diketahui bahwa masing-masing membran mempunyai gugus hidroksil, tetapi serapannya semakin melebar untuk setiap membran. Gugus alkil dan karboksil juga masih tampak, namun pada membran nata teraktivasi serapannya berkurang, sedangkan pada membran nata-en muncul puncak baru yang menunjukkan adanya gugus amin. Hal ini membuktikan bahwa telah terjadi ikatan antara nata dengan etilendiamin. Fibers contained in nata Aloe vera is cellulose that can be used in the synthesis of membrane. The research has done on the synthesis of nata Aloe vera-ethylenediamine (nata-en membrane by using liquid vacuum system. Membrane preparation process consisted of three stages, they are nata Aloe vera preparation, activation using sulfuric acid, and membrane modification by using ethylenediamine. The purpose of research is to study the technique of nata-en membrane synthesis then to perform the characterization to determine the character of their structure.The results

  11. Effects of massage treatment combined with topical cactus and aloe on puerperal milk stasis.

    Science.gov (United States)

    Meng, Shan; Deng, Qingchun; Feng, Chunyu; Pan, Yinglian; Chang, Qing

    2015-01-01

    Puerperal milk stasis is one of the most common puerperal complication that directly affects breastfeeding. Massage treatment with topical cactus and aloe for puerperal milk stasis might be a superior treatment, and it does not affect breastfeeding. The intervention group was treated with massages with cactus and aloe cold compresses, and the control group was treated with massage treatment or cactus and aloe cold compresses alone. We evaluated the efficacies of the treatments through comparisons of the feeding patterns, hardness, and pain after treatment between the three groups. We found that breastfeeding rates were significantly increased in the massage combine with combined with cactus and aloe cold compress group (P massage combine with combined with cactus and aloe cold compress group than in the massage or cold compress group (P Massage treatment with topical cactus and aloe topical effectively improved the pain status, hard lump of puerperal milk stasis and increase breastfeeding rate.

  12. Efficacy of aloe vera gel as an adjuvant treatment of oral submucous fibrosis.

    Science.gov (United States)

    Alam, Sarwar; Ali, Iqbal; Giri, K Y; Gokkulakrishnan, S; Natu, Subodh S; Faisal, Mohammad; Agarwal, Anshita; Sharma, Himanshu

    2013-12-01

    Definitive therapy is not defined for the management of oral submucous fibrosis (OSMF). This study evaluated the efficacy of aloe vera gel as an adjuvant treatment of OSMF. A double-blind, placebo-controlled, parallel-group randomized controlled trial was conducted on 60 subjects with OSMF divided into medicinal treatment (submucosal injection of hyaluronidase and dexamethasone, n = 30) and surgical treatment (n = 30) categories. Each category was randomly divided into groups A (with aloe vera, n = 15 per category) and B (without aloe vera, n = 15 per category). Follow-up assessment for various symptoms was performed, and results were analyzed using paired and unpaired Student t tests. The group receiving aloe vera had a significant improvement in most symptoms of OSMF (P aloe vera group, in both the medicinal and surgical categories. Aloe vera gel was effective as an adjuvant in treatment of OSMF. Copyright © 2013 Elsevier Inc. All rights reserved.

  13. PHENOTYPE IDENTIFICATION OF TYPES Aloe sp. PLANT IN THE SPECIAL REGION OF YOGYAKARTA

    OpenAIRE

    Maria Theresia Darini

    2014-01-01

    The study aims to determine the types, population, frequency and dominance Aloe sp. plant has been conducted in Yogyakarta, from March up to September 2012. Research carried out by the method of vegetation analysis through surveys and sampling of the Aloe sp. plant locations, districts in the regency and in the province Special Region of Yogyakarta. To determine the types of Aloe sp. plants using descriptions that are tailored to the identification of key observations dichotomi...

  14. The effect of Aloe vera leaf gel on fatty streak formation in hypercholesterolemic rabbits.

    Science.gov (United States)

    Dana, Nasim; Javanmard, Shaghayegh Haghjooy; Asgary, Sedigheh; Asnaashari, Hossein; Abdian, Narges

    2012-05-01

    Atherosclerosis is a complex disease that is associated with a variety of etiologic factors such as hyperlipidemia and inflammation. Aloe vera (Liliaceae family) has been used traditionally as an anti-inflammatory drug. The aims of this survey were to define the beneficial effects of Aloe vera leaf gel on some of the atherosclerosis risk factors, and also fatty streak formation in hypercholesterolemic rabbits. [corrected] 32 white male rabbits were randomly divided into four experimental groups (n = 8, each). During the study, the animals had a standard diet (control group), high cholesterol diet (HC group), high cholesterol diet with Aloe vera leaf gel (3.2%v/v) (HC+ Aloe group) and Aloe vera leaf gel (Aloe group) for 30 days. Fasting blood samples were collected from all animals at the beginning and end of the study. Then total cholesterol (TC), fasting blood sugar (FBS), triglyceride (TG) and CRP were measured before and after experimental periods. By the end of the study, the aortas were removed and investigated for atherosclerosis plaque formation. Significant differences were observed in TC and CRP levels of the high-cholesterol diet with Aloe vera and the high-cholesterol diet alone (p Aloe vera(p Aloe vera has beneficial effects on the prevention of fatty streak development; it may reduce the development of atherosclerosis through modification of risk factors. However, further studies are needed to understand the mechanisms whereby this plant exerts its anti-atherosclerotic effects.

  15. Analysis of the anthraquinones aloe-emodin and aloin by gas chromatography/mass spectrometry.

    Science.gov (United States)

    ElSohly, Mahmoud A; Gul, Waseem; Murphy, Timothy P

    2004-12-20

    A procedure was developed for the determination of low levels of the anthraquinones aloe-emodin and aloin A (barbalin) in aloe products based on gas chromatography/mass spectrometry (GC/MS) of the trimethyl silyl (TMS) derivatives of these analytes in the presence of Chrysophanol used as internal standard. The method was used to analyze several aloe based commercial products (liquids, gels and solids). Wide variation in the level of these anthraquinones was observed among the different products. The method had a sensitivity of 0.005 ppm of aloe-emodin and 0.05 ppm of aloin.

  16. Development of Aloe vera based edible coating for tomato

    Science.gov (United States)

    Athmaselvi, K. A.; Sumitha, P.; Revathy, B.

    2013-12-01

    The effect of formulated Aloe vera based edible coating on mass loss, colour, firmness, pH, acidity, total soluble solid, ascorbic acid and lycopene on the coated tomato was investigated. The tomato in control showed a rapid deterioration with an estimated shelf life period of 19 days, based on the mass loss, colour changes, accelerated softening and ripening. On the contrary, the coating on tomatoes delayed the ripening and extended the shelf life up to 39 days. The physiological loss in weight was 7.6 and 15.1%, firmness was 36 and 46.2 N on 20th day for control and coated tomatoes, respectively. From the results, it was concluded that the use of Aloe vera based edible coating leads to increased tomato shelf-life.

  17. Memristors in the electrical network of Aloe vera L.

    Science.gov (United States)

    Volkov, Alexander G; Reedus, Jada; Mitchell, Colee M; Tucket, Clayton; Forde-Tuckett, Victoria; Volkova, Maya I; Markin, Vladislav S; Chua, Leon

    2014-01-01

    A memristor is a resistor with memory, which is a non-linear passive two-terminal electrical element relating magnetic flux linkage and electrical charge. Here we found that memristors exist in vivo. The electrostimulation of the Aloe vera by bipolar sinusoidal or triangle periodic waves induce electrical responses with fingerprints of memristors. Uncouplers carbonylcyanide-3-chlorophenylhydrazone and carbonylcyanide-4-trifluoromethoxy-phenyl hydrazone decrease the amplitude of electrical responses at low and high frequencies of bipolar periodic sinusoidal or triangle electrostimulating waves. Memristive behavior of an electrical network in the Aloe vera is linked to the properties of voltage gated ion channels: the K+ channel blocker TEACl reduces the electric response to a conventional resistor. Our results demonstrate that a voltage gated K+ channel in the excitable tissue of plants has properties of a memristor. The discovery of memristors in plants creates a new direction in the modeling and understanding of electrical phenomena in plants. PMID:25763487

  18. Aloe vera in the management of oral submucous fibrosis

    Directory of Open Access Journals (Sweden)

    Vagish Kumar L Shanbhag

    2017-06-01

    Full Text Available Oral submucous fibrosis is a pre-malignant condition commonly prevalent in India, Pakistan, Taiwan and Sri Lanka. Patients characteristically exhibit progressive reduction in mouth opening. The disease has a high malignant potential and thus should be diagnosed and treated at the earliest. However, none of the currently used medical therapies show promising results. The current article briefly reviews the role of Aloe vera in the management of oral submucous fibrosis.

  19. Comparative in vitro inhibitory effects of cold extracts of some ...

    African Journals Online (AJOL)

    Cold extracts of five fungicidal plants (Aloe barbadensis, Azdrichta indica, Nicotiana tabacum, Tridax precubens and Carica papaya) were screened for in vitro inhibitory effects on Fusarium oxysporium, using 25, 50, 75 and 100% concentrations of each extract. The four different concentrations of N. tabacum completely ...

  20. Soluble epoxide hydrolase inhibitory activity of anthraquinone components from Aloe.

    Science.gov (United States)

    Sun, Ya Nan; Kim, Jang Hoon; Li, Wei; Jo, A Reum; Yan, Xi Tao; Yang, Seo Young; Kim, Young Ho

    2015-10-15

    Aloe is a short-stemmed succulent herb widely used in traditional medicine to treat various diseases and as raw material in cosmetics and heath foods. In this study, we isolated and identified two new anthraquinone derivatives, aloinoside C (6) and aloinoside D (7), together with six known compounds from an aqueous dissolved Aloe exudate. Their structures were identified by spectroscopic analysis. The inhibitory effects of the isolated compounds on soluble epoxide hydrolase (sEH) were evaluated. Compounds 1-8 inhibited sEH activity potently, with IC50 values ranging from 4.1±0.6 to 41.1±4.2 μM. A kinetic analysis of compounds 1-8 revealed that the inhibitory actions of compounds 1, 6 and 8 were non-competitive, whereas those of compounds 2-5 and 7 were the mixed-type. Molecular docking increases our understanding of receptor-ligand binding of all compounds. These results demonstrate that compounds 1-8 from Aloe are potential sEH inhibitors. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Encapsulation of Aloe Vera and Its Effect During Yogur Incubation

    Directory of Open Access Journals (Sweden)

    Ricardo Adolfo Parra Huertas

    2014-11-01

    Full Text Available The yogurt is milk derivative highly consumed around the world,as well as aloe vera. Both have reports tocontribute to human health. The purpose of this research is to determine the effect of the addition of capsules with aloe vera during the incubation of yogurt. Aloeverawas encapsulated in alginate at two different concentrations, 1% and 2%,addingthe capsules from the moment of incubation and comparing the effect of the addition of capsules withthe non-addition of them. For these samples were determined: pH, acidity, syneresis, lactic acid bacteria count, sensory evaluation and proximate analysis. The results indicated that for the three treatments pH values and acid behaved similarly to each characteristic of the yogurt during incubation. The lactic acid bacteria count indicated that treatment with capsules containing 2% sodium alginate had higher counts. Sensorially, three treatments had a favorable acceptability; proximate analysis had favorable values . In conclusion,the tests showed the viability of encapsulated aloe vera in the manufacture of yogurt during incubation time without being affected by the concentration of sodium alginate.

  2. Investigation of Land Subsidence using ALOS PALSAR data: a case study in Mentougou (Beijing, China)

    Science.gov (United States)

    Chen, Jianping; Xiang, Jie; Xie, Shuai; Liu, Jing; Tarolli, Paolo

    2017-04-01

    Mining activities have been documented for centuries in Mentougou, and land subsidence resulting from mining operations has already been known over the past few decades. However, there has been ongoing concern that excessive groundwater extraction may lead to further subsidence. Therefore it is critical to map the land cover changes to understand the actual impact of these activities. So, the land cover changes from 2006 to 2011 were examined based on multi-source remote sensing imageries( including ALOS and landsat-7) by using object-oriented classifications combined with a decision tree and retrospective approaches. Also, land subsidence in Mentougou between 2006 and 2011 has been mapped using the interferometric synthetic aperture radar (InSAR) time-series analysis with the ALOS L-band SAR data. We processed 14 ascending SAR images during May 2006 to July 2011. Comparison of InSAR measurements with the land cover changes and pre-existing faults suggest that mining activities is the main cause of land subsidence. The land subsidence observed from InSAR data are approximately up to 15 mm/year in open-pit mining area and up to 24 mm/year in underground mining areas. The InSAR result are validated by the ground survey data in several areas, and the comparison between the InSAR result with the mining schedule showed there were some correlations between them. The result underline the potential use of InSAR measurements to provide better investigation for land subsidence, and also suggest that the most influential factors for land subsidence is underground coal mine.

  3. Blood glucose lowering activity of aloe based composition, UP780, in alloxan induced insulin dependent mouse diabetes model

    National Research Council Canada - National Science Library

    Yimam, Mesfin; Zhao, Jifu; Corneliusen, Brandon; Pantier, Mandee; Brownell, Lidia; Jia, Qi

    2014-01-01

    .... Previously it has been reported that UP780, a standardized composition of aloe chromone formulated with an aloe polysaccharide, has a significant impact in reducing HbA1C, fasting blood glucose...

  4. Effects of ethanol on the pharmacokinetics of extended-release oxycodone with sequestered naltrexone (ALO-02).

    Science.gov (United States)

    Malhotra, Bimal K; Matschke, Kyle; Wang, Qiang; Bramson, Candace; Salageanu, Joanne

    2015-04-01

    ALO-02 capsules, intended to deter abuse, contain pellets of extended-release oxycodone hydrochloride (HCl), an opioid agonist, surrounding sequestered naltrexone HCl, an opioid antagonist. The objective of this study was to determine the effects of administration of ALO-02 with 20 or 40 % ethanol on the pharmacokinetics of oxycodone. This was an open-label, single-dose, randomized, three-way crossover study in 18 healthy fasting adults administered ALO-02 20/2.4 mg (oxycodone/naltrexone) with water, 20 % ethanol, or 40 % ethanol, each under naltrexone block. Median time to maximum concentration was 12 h postdose when ALO-02 was administered with water or 20 % ethanol and decreased to 8 h postdose with 40 % ethanol. Geometric mean area under the plasma concentration-time curve (AUC) from time zero extrapolated to infinity (AUC∞) and maximum concentration (Cmax) values were similar for ALO-02 administered with water or 20 % ethanol, and increased by about 13 and 37 %, respectively, for ALO-02 administered with 40 % ethanol versus water. The 90 % confidence intervals (CIs) for AUC∞ and Cmax ratios of ALO-02 with 20 % ethanol versus water were within 80-125 %; upper 90 % CIs were >125 % for ALO-02 with 40 % ethanol versus water. The most common adverse events were mild-to-moderate vomiting, nausea, headache, and somnolence. Incidence of adverse events increased for ALO-02 given with ethanol versus water. Oxycodone exposures (Cmax) were unaffected when ALO-02 was administered with 20 % ethanol but Cmax increased by 37 % with 40 % ethanol versus water. ALO-02 administered with ethanol under naltrexone block was generally well tolerated.

  5. Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.

    Science.gov (United States)

    Tummalapalli, Mythili; Berthet, Morgane; Verrier, Bernard; Deopura, B L; Alam, M S; Gupta, Bhuvanesh

    2016-01-01

    Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Comparative evaluation of Bacillus licheniformis 5A5 and Aloe variegata milk-clotting enzymes

    Directory of Open Access Journals (Sweden)

    S. A. Ahmed

    2012-03-01

    Full Text Available The properties of a milk clotting enzyme (MCE produced by bacteria (Bacillus licheniformis 5A5 were investigated and compared to those of rennet extracted from a plant (Aloe variegata. Production of MCE by B. licheniformis 5A5 was better in static than in shaken cultures. Maximum activity (98.3 and 160.3 U/ml of clotting was obtained at 75ºC and 80ºC with bacterial and plant rennet, respectively. In the absence of substrate, the clotting activity of Aloe MCE was found to be less sensitive to heat inactivation up to 80ºC for 75 min, retaining 63.8% of its activity, while bacterial MCE was completely inhibited. CaCl2 stimulated milk clotting activity (MCA up to 2% and 1.5% for bacterial and plant enzymes. NaCl inhibited MCA for both enzymes, even at low concentration (1%. Plant MCE was more sensitive to NaCl at 3% concentration it retained 30.2% of its activity, whereas bacterial MCE retained 64.1%. Increasing skim milk concentration caused a significant increase in MCA up to 6% for both enzymes. Mn2+ stimulated the activity of bacterial and plant enzymes to 158.6 and 177.9%, respectively. EDTA and PMSF increased the activity of plant MCE by 34.4 and 41.1%, respectively, which is higher than those for the bacterial MCE (19.1 and 20.9%. Some natural materials activated MCE, the highest activation of bacterial MCE (128.1% was obtained in the presence of Fenugreek (with acid extraction. However Lupine Giza 1 (with neutral extraction gave the highest activation of plant MCE (137.9%. All extracts from Neem plant increased MCA at range from 105.6% to 136.4%. Plant MCE exhibited much better stability when stored at room temperature (25-30ºC for 30 days, retaining 51.2% of its activity. Bacterial MCE was highly stabile when stored under freezing (-18ºC, retaining 100% of its activity after 30 days. Moreover, bacterial MCE was highly tolerant to repeated freezing and thawing without loss of activity for 8 months.

  7. Evaluación de la Actividad Antifúngica In Vitro de Emulsiones de Marco (Ambrosia Arborescens Mill) y Matico (Aristeguietia Glutinosa Lam) sobre Hongos Patógenos causantes de la Dermatomicosis

    OpenAIRE

    Dávila Molina, Clara Gisela; Pazos Gambarroti, Lourdes Angélica

    2015-01-01

    The pharmaceutical and cosmetic industries in Ecuador are interested in developing natural products, for that reason in this study was considered the evaluation of antifungal activity in vitro of natural ingredients as Marco (Ambrosia arborescens Mill) and Matico (Aristeguietia glutinosa Lam) in cosmetic formulations. Three types of cosmetic formulation were considered: cream, lotion, gel, via two experimental designs for analyzing the preliminary stability before of the evaluation of antifun...

  8. Improvement of glucose and lipid profile status with Aloe vera in pre-diabetic subjects: a randomized controlled-trial.

    Science.gov (United States)

    Alinejad-Mofrad, Samaneh; Foadoddini, Mohsen; Saadatjoo, Seyed Alireza; Shayesteh, Majid

    2015-01-01

    Pre-diabetes is a disturbing trend in the population, who are at risk of developing type-two diabetes. The aim of this study was to determine the effects use of Aloe vera in different doses on glucose and lipid profile in pre-diabetic subjects. This study was a double blind randomized controlled trial (72 subjects) with pre-diabetes symptoms in 3 groups consumed capsules twice a day: Aloe vera 300 mg (AL300), 500 mg (AL500) and placebo (PL). Fasting blood glucose (FBS), HbA1C and lipid profile were evaluated in baseline, 4 or 8 weeks. On-way ANOVA, Friedman, Wilcoxon, Kruskal-Wallis , Mann-Whitney and Chi-square tests were used for within or between groups statistical analysis. FBS level in group AL300, showed significantly decreased in fourth week after the intervention, compared to PL in the same time (p = 0.001). Also, HbA1C level in this group at the eighth week after the intervention (p = 0.042), had a significant decrease. The levels of Total cholesterol and LDL-C, only in the group AL500 (p < 0.001 and p = 0.01), was significantly reduced, along with HDL-C level improvement just after eight weeks (p = 0.004). Triglyceride level showed a significant decrease (p < 0.045) just after four weeks use of AL500. The Use of Aloe vera extract in pre-diabetic patients, could revert impaired blood glucose within four weeks, but after eight weeks could alleviate their abnormal lipid profile.

  9. Emodin and Aloe-Emodin Suppress Breast Cancer Cell Proliferation through ERα Inhibition

    Science.gov (United States)

    Huang, Pao-Hsuan; Huang, Chih-Yang; Chen, Mei-Chih; Lee, Yueh-Tsung; Yue, Chia-Herng; Wang, Hsin-Yi

    2013-01-01

    The anthraquinones emodin and aloe-emodin are abundant in rhubarb. Several lines of evidence indicate that emodin and aloe-emodin have estrogenic activity as phytoestrogens. However, their effects on estrogen receptor α (ERα) activation and breast cancer cell growth remain controversial. The goal of this study is to investigate the effects and molecular mechanisms of emodin and aloe-emodin on breast cancer cell proliferation. Our results indicate that both emodin and aloe-emodin are capable of inhibiting breast cancer cell proliferation by downregulating ERα protein levels, thereby suppressing ERα transcriptional activation. Furthermore, aloe-emodin treatment led to the dissociation of heat shock protein 90 (HSP90) and ERα and increased ERα ubiquitination. Although emodin had similar effects to aloe-emodin, it was not capable of promoting HSP90/ERα dissociation and ERα ubiquitination. Protein fractionation results suggest that aloe-emodin tended to induce cytosolic ERα degradation. Although emodin might induce cytosolic ERα degradation, it primarily affected nuclear ERα distribution similar to the action of estrogen when protein degradation was blocked. In conclusion, our data demonstrate that emodin and aloe-emodin specifically suppress breast cancer cell proliferation by targeting ERα protein stability through distinct mechanisms. These findings suggest a possible application of anthraquinones in preventing or treating breast cancer in the future. PMID:23864887

  10. The Effect of Aloe vera Plus on the Liver: A Pharmacovigilance ...

    African Journals Online (AJOL)

    The transparent gel of Aloe vera has been used as a nutritional supplement and herbal remedy for centuries. It is claimed to have several therapeutic properties but there is little scientific evidence of its effectiveness and safety. The aim of this study is to investigate the effects of GNLD's Aloe vera plus on the liver of rats.

  11. Pengeringan Lidah Buaya (AloVera Menggunakan Oven Gelombang Mikro (Microwave Oven

    Directory of Open Access Journals (Sweden)

    Edy Hartulistiyoso

    2011-10-01

    Full Text Available Aloe vera (Aloe vera is known as a useful plant, both as food, cosmetics or pharmaceuticals. Due its high water content, Aloe vera should be processed immediately after harvest. This paper will discuss the drying of aloe vera using microwave oven. The drying process of 50 gr Aloe vera gel and rind using 80 Watts microwave power completed in 140 minutes to reach the final moisture content of 7% wet basis. The drying process in microwave drying shows similar process to that of conventional drying. Dipolar rotation mechanism of water molecules at microwave drying does not affect the drying stage. It shows however shorter process time. The water removal of the drying material occurred in the early minutes until the 50th minute. This because of the high free water surface on the material, while from minute 50 to minute 140 slowed the drying process. Highest temperature of the material during the process is achieved at 57.6 oC in drying of gel and 70.7 oC in drying of Aloe vera rind. It is expected that there is no damage of nutritional content during drying in the this temperature range. Quality analysis of flour and tea of Aloe vera after drying by microwave showed that Aloe vera powder produced within the range of the standard, both visually and microbiologically, but indicated as low quality when viewed from acid content levels. Whether microwave heating mechanism affects the acidity, this needs further study.

  12. The effects of Aloe vera [gel] on clotting time, prothrombin time and ...

    African Journals Online (AJOL)

    Background: Published reports on the effects of Aloe vera gel on blood coagulation in experimental animals are relatively scanty. Aim: To determine the effect of Aloe vera gel on clotting time, prothrombin time and plasma fibrinogen concentration in albino Wistar rats. Methods: A total of 28 adult albino Wistar rats divided ...

  13. Optimisation of nitrogen and potassium for Aloe vera (L.) Burm.f. in a ...

    African Journals Online (AJOL)

    Aloe vera (L.) Burm.f. is highly appreciated due to its short growth period and high economic value among all Aloe species, and is used in pharmaceuticals, folk medicine, healthcare, cosmetic products and food products. Hydroponic systems have the potential to improve the cropping management and to achieve higher ...

  14. Bacterial leaf rot of Aloe vera L., caused byErwinia chrysanthemi biovar 3

    NARCIS (Netherlands)

    Laat, de P.C.A.; Verhoeven, J.T.W.; Danse, J.D.

    1994-01-01

    A severe attack of the bacteriumErwinia chrysantemi biovar 3 on the succulentAloe vera on the Carribean island of Aruba is described. Biochemical and pathological characteristics of strains are presented, including results of successful inoculation experiments onAloe vera. This is the first report

  15. A revised generic classification for Aloe (Xanthorrhoeaceae subfam. Asphodeloideae)

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Klopper, Ronell R.; Smith, Gideon F.

    2013-01-01

    of the traditionally broadly circumscribed genus Aloe, a charismatic Old World group of leaf succulents, has necessitated nomenclatural adjustments. We propose a narrower generic concept for Aloe s. str. and the recognition of segregate genera to reflect accumulating evidence for monophyletic groups: here, the genus...

  16. Effect of Some Plant Extracts on the Microbial Spoilage of Cajanus ...

    African Journals Online (AJOL)

    The effect of ethanolic extracts of seven plant sources on the microbial spoilage of Cajanus cajan extract was investigated. The results showed that the extracts obtained from Aloe vera, bitter leaf, Gultiferae (garcinia or bitter kola), Ocimum gratissimum (scent leaf) and Zingiber officialae (ginger) were effective against ...

  17. Aloe QDM complex enhances specific cytotoxic T lymphocyte killing in vivo in metabolic disease mice.

    Science.gov (United States)

    Lee, Youngjoo; Kim, Jiyeon; An, Jinho; Lee, Heetae; Kong, Hyunseok; Song, Youngcheon; Shin, Eunju; Do, Seon-Gil; Lee, Chong-Kil; Kim, Kyungjae

    2017-03-01

    We developed spontaneous diet-induced metabolic disease in mice by feeding them a high-fat diet for 23 weeks and administered Aloe QDM complex for 16 weeks to examine its restorative effect on immune disorders and metabolic syndrome. A series of immune functional assays indicated Aloe QDM complex enhanced lymphocyte proliferation and antigen-specific immunity as determined by the restored functions of cytotoxic T lymphocytes (CTL) and IgG production. The elevated serum TNF-α level was also regulated by Aloe QDM complex treatment, which suggested its complex therapeutic potential. As for metabolic phenotypes, oral administration of Aloe QDM complex significantly improved diabetic symptoms, including high fasting glucose levels and glucose tolerance, and distinctly alleviated lipid accumulation in adipose and hepatic tissue. The simultaneous restoration of Aloe QDM complex on metabolic syndrome and host immune dysfunction, especially on the specific CTL killing was first elucidated in our study.

  18. In vitro wound healing and cytotoxic activity of the gel and whole-leaf materials from selected aloe species.

    Science.gov (United States)

    Fox, Lizelle T; Mazumder, Anisha; Dwivedi, Anupma; Gerber, Minja; du Plessis, Jeanetta; Hamman, Josias H

    2017-03-22

    Aloe vera is one of the most important medicinal plants in the world with applications in the cosmetic industry and also in the tonic or health drink product market. Different parts of Aloe ferox and Aloe marlothii are used as traditional medicines for different applications. Although wound healing has been shown for certain aloe gel materials (e.g. A. vera ) previously, there are conflicting reports on this medicinal application of aloe leaf gel materials. The present study aimed at determining the wound healing properties of the gel and whole-leaf materials of Aloe vera, Aloe ferox and Aloe marlothii, as well as their cytotoxic effects on normal human keratinocyte cells (HaCaT). Nuclear magnetic resonance spectroscopy was used to chemically fingerprint the aloe gel and whole-leaf materials by identifying characteristic marker molecules of aloe gel and whole-leaf materials. An MTT assay was performed to determine the cytotoxicity of the various aloe whole-leaf and gel materials on HaCaT cells. Wound healing and in vitro cell migration were investigated with HaCaT cells by means of the CytoSelect™ assay kit. The in vitro wound healing assay suggested that all the aloe gel and whole-leaf materials examined, exhibited faster wound healing activity than the untreated control group. After 48h, all the aloe gel and whole-leaf materials almost completely caused full wound closure, displaying 98.07% (A. marlothii whole-leaf), 98.00% (A. vera gel), 97.20% (A. marlothii gel), 96.00% (A. vera whole-leaf), 94.00% (A. ferox gel) and 81.30% (A. ferox whole-leaf) wound closure, respectively. It was noteworthy that the gel materials of all the three aloe species exhibited significantly faster (pAloe species showed negligible toxicity towards the HaCaT cells. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  19. Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean.

    Science.gov (United States)

    Zhang, Fuli; Ge, Honglian; Zhang, Fan; Guo, Ning; Wang, Yucheng; Chen, Long; Ji, Xiue; Li, Chengwei

    2016-03-01

    Sclerotinia stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary is a major disease of soybean (Glycine max (L.) Merr.). At present, we revealed the three-way interaction between Trichoderma harzianum T-aloe, pathogen S. sclerotiorum and soybean plants in order to demonstrate biocontrol mechanism and evaluate biocontrol potential of T-aloe against S. sclerotiorum in soybean. In our experiments, T-aloe inhibited the growth of S. sclerotiorum with an efficiency of 56.3% in dual culture tests. T-aloe hyphae grew in parallel or intertwined with S. sclerotiorum hyphae and produced hooked contact branches, indicating mycoparasitism. Plate tests showed that T-aloe culture filtrate inhibited S. sclerotiorum growth with an inhibition efficiency of 51.2% and sclerotia production. T-aloe pretreatment showed growth-promoting effect on soybean plants. The activities of peroxidase, superoxide dismutase, and catalase increased, and the hydrogen peroxide (H2O2) as well as the superoxide radical (O2(-)) content in soybean leaves decreased after T-aloe pretreatment in response to S. sclerotiorum pathogen challenge. T-aloe treatment diminished damage caused by pathogen stress on soybean leaf cell membrane, and increased chlorophyll as well as total phenol contents. The defense-related genes PR1, PR2, and PR3 were expressed in the leaves of T-aloe-treated plants. In summary, T-aloe displayed biocontrol potential against S. sclerotiorum. This is the first report of unraveling biocontrol potential of Trichoderma Spp. to soybean sclerotinia stem rot from the three-way interaction between the biocontrol agent, pathogen S. sclerotiorum and soybean plants. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  20. Acute toxic hepatitis caused by an aloe vera preparation in a young patient: a case report with a literature review.

    Science.gov (United States)

    Lee, Jeonghun; Lee, Mi Sun; Nam, Kwan Woo

    2014-07-01

    Aloe is one of the leading products used in phytomedicine. Several cases of aloe-induced toxic hepatitis have been reported in recent years. However, its toxicology has not yet been systematically described in the literature. A 21-year-old female patient was admitted to our hospital with acute hepatitis after taking an aloe vera preparation for four weeks. Her history, clinical manifestation, laboratory findings, and histological findings all led to the diagnosis of aloe vera-induced toxic hepatitis. We report herein on a case of acute toxic hepatitis induced by aloe vera.

  1. ESTIMATION OF DAMAGED AREAS DUE TO THE 2010 CHILE EARTHQUAKE AND TSUNAMI USING SAR IMAGERY OF ALOS/PALSAR

    Directory of Open Access Journals (Sweden)

    P. J. Ni Made

    2016-06-01

    Full Text Available A large-scale earthquake and tsunami affect thousands of people and cause serious damages worldwide every year. Quick observation of the disaster damage is extremely important for planning effective rescue operations. In the past, acquiring damage information was limited to only field surveys or using aerial photographs. In the last decade, space-borne images were used in many disaster researches, such as tsunami damage detection. In this study, SAR data of ALOS/PALSAR satellite images were used to estimate tsunami damage in the form of inundation areas in Talcahuano, the area near the epicentre of the 2010 Chile earthquake. The image processing consisted of three stages, i.e. pre-processing, analysis processing, and post-processing. It was conducted using multi-temporal images before and after the disaster. In the analysis processing, inundation areas were extracted through the masking processing. It consisted of water masking using a high-resolution optical image of ALOS/AVNIR-2 and elevation masking which built upon the inundation height using DEM image of ASTER-GDEM. The area result was 8.77 Km2. It showed a good result and corresponded to the inundation map of Talcahuano. Future study in another area is needed in order to strengthen the estimation processing method.

  2. ALOE MACULATA (XANTHORROEACEAE, PRIMER REGISTRO PARA LA FLORA ARGENTINA

    Directory of Open Access Journals (Sweden)

    Marcelo D. Arana

    2012-01-01

    Full Text Available Aloe maculata, una monocotiledónea petaloidea originaria de Sudáfrica, se cita por primera vez como miembro de la flora espontánea de Argentina. Esta especie crece en diferentes ambientes de la provincia de Córdoba, Argentina. Los objetivos del trabajo son determinar su estatus de residencia y proponer caracteres diagnósticos novedosos para su identificación, junto con observaciones ecológicas. Se ilustra la especie y se provee un mapa de distribución.

  3. Gold nanoparticles prepared using cape aloe active components.

    Science.gov (United States)

    Krpetić, Zeljka; Scarì, Giorgio; Caneva, Enrico; Speranza, Giovanna; Porta, Francesca

    2009-07-07

    A novel use of two components of Cape aloe, aloin A and aloesin, acting as stabilizers in the preparation of gold and silver nanoparticles, is reported. Stable water-soluble particles of different size and shape are prepared by varying the reaction conditions, temperature, reaction time, and reducing agents. Characterization of the obtained particles is performed using UV-visible, attenuated total reflection Fourier transform infrared (ATR-FTIR), and 1H NMR spectroscopies and transmission electron microscopy (TEM). The efficient cellular uptake of 50 nm sized aloin A and aloesin stabilized gold particles into macrophages and HeLa cells was investigated, proposing these particles as nanovehicles.

  4. Deformation Survey of Volcanoes in Central America Using ALOS

    Science.gov (United States)

    Cao, Y. M.; Amelung, F.; Li, Z.

    2016-12-01

    The Japanese L-Band satellite ALOS-1 has proven a useful tool to for deformation monitoring of active volcanoes. Deformation surveys in volcanic arcs sound the world have detected a multitude of deformation signals that have led to improved understanding of the volcanic systems. Here we present a systematic deformation survey of volcanoes in central America for the 2007-2011 time frame. We use a small-baseline time-series approach and correct for atmospheric delays using global numerical weather models.

  5. The Penetration Depth Derived from the Synthesis of ALOS/PALSAR InSAR Data and ASTER GDEM for the Mapping of Forest Biomass

    Directory of Open Access Journals (Sweden)

    Wenjian Ni

    2014-08-01

    Full Text Available The Global Digital Elevation Model produced from stereo images of Advanced Spaceborne Thermal Emission and Reflection Radiometer data (ASTER GDEM covers land surfaces between latitudes of 83°N and 83°S. The Phased Array type L-band Synthetic Aperture Radar (PALSAR onboard Advanced Land Observing Satellite (ALOS collected many SAR images since it was launched on 24 January 2006. The combination of ALOS/PALSAR interferometric data and ASTER GDEM should provide the penetration depth of SAR data assuming ASTER GDEM was the elevation of vegetation canopy top. It would be correlated with forest biomass because penetration depth could be affected by forest density and forest canopy height. Their combination held great promises for the forest biomass mapping over large area. The feasibility of forest biomass mapping through the data synthesis of ALOS/PALSAR InSAR data and ASTER GDEM was investigated in this study. A procedure for the extraction of penetration depth was firstly proposed. Then three models were built for biomass estimation: (I model only using backscattering coefficients of ALOS/PALSAR data; (II model only using penetration depth; (III model using both of them. The biomass estimated from Lidar data was taken as reference data to evaluate the three different models. The results showed that the combination of backscattering coefficients and penetration depth gave the best accuracy. The forest disturbance has to be considered in forest biomass estimation because of the long time span of ASTER data for generating ASTER GDEM. The spatial homogeneity could be used to improve estimation accuracy.

  6. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, Aloe Vera and Propolis in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: An in vitro study

    Directory of Open Access Journals (Sweden)

    Anuj Bhardwaj

    2013-01-01

    Full Text Available Aim: Present study evaluated the efficacy of natural derivative irrigants, Morinda citrifolia juice (MCJ, Aloe Vera and Propolis in comparison to 1% sodium hypochlorite with passive ultrasonic irrigation for removal of the intraradicular E. faecalis biofilms in extracted single rooted human permanent teeth. Materials and Methods: Biofilms of E. faecalis were grown on the prepared root canal walls of 60 standardized root halves which were longitudinally sectioned. These root halves were re-approximated and the samples were divided into five groups of twelve each. The groups were, Group A (1% NaOCl, Group B (MCJ, Group C (Aloe vera, Group D (Propolis and Group E (Saline. These groups were treated with passive ultrasonic irrigation (PUI along with the respective irrigants. The root halves were processed for scanning electron microscopy. Three images (X2.5, coronal, middle and apical, were taken for the twelve root halves in each of the five groups. The images were randomized and biofilm coverage assessed independently by three calibrated examiners, using a four-point scoring system. Results: 1% NaOCl with passive ultrasonic irrigation (PUI was effective in completely removing E. faecalis biofilm and was superior to the natural irrigants like MCJ, Aloe vera and Propolis tested in this study. Conclusion: 1% NaOCl used along with passive ultrasonic irrigation was effective in completely removing E. faecalis biofilm when compared to natural irrigants (MCJ, Aloe Vera and Propolis.

  7. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, Aloe Vera and Propolis) in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: an in vitro study.

    Science.gov (United States)

    Bhardwaj, Anuj; Velmurugan, Natanasabapathy; Ballal, Suma

    2013-01-01

    Present study evaluated the efficacy of natural derivative irrigants, Morinda citrifolia juice (MCJ), Aloe Vera and Propolis in comparison to 1% sodium hypochlorite with passive ultrasonic irrigation for removal of the intraradicular E. faecalis biofilms in extracted single rooted human permanent teeth. Biofilms of E. faecalis were grown on the prepared root canal walls of 60 standardized root halves which were longitudinally sectioned. These root halves were re-approximated and the samples were divided into five groups of twelve each. The groups were, Group A (1% NaOCl), Group B (MCJ), Group C (Aloe vera), Group D (Propolis) and Group E (Saline). These groups were treated with passive ultrasonic irrigation (PUI) along with the respective irrigants. The root halves were processed for scanning electron microscopy. Three images (X2.5), coronal, middle and apical, were taken for the twelve root halves in each of the five groups. The images were randomized and biofilm coverage assessed independently by three calibrated examiners, using a four-point scoring system. 1% NaOCl with passive ultrasonic irrigation (PUI) was effective in completely removing E. faecalis biofilm and was superior to the natural irrigants like MCJ, Aloe vera and Propolis tested in this study. 1% NaOCl used along with passive ultrasonic irrigation was effective in completely removing E. faecalis biofilm when compared to natural irrigants (MCJ, Aloe Vera and Propolis).

  8. O uso do Aloe sp (aloe vera) em feridas agudas e crônicas: revisão integrativa

    OpenAIRE

    Lucélia Terra Chini; Roberta Aparecida Mendes; Lais Reis Siqueira; Sandreli Pereira da Silva; Patrícia Costa dos Santos Silva; Eliza Maria Rezende Dázio; Silvana Maria Coelho Leite Fava

    2017-01-01

    Objetivos: buscar evidencias disponibles en la literatura acerca del uso de Aloe sp (sábila) en la cicatrización de heridas agudas y crónicas. Materiales y método: se trata de una revisión integradora realizada en las bases de datos LILACS, PubMed y Scopus, en el periodo de febrero a marzo del 2015. La búsqueda resultó en 178 publicaciones. Resultados: siete estudios constituyeron la revisión, los cuales involucraron personas con heridas de cesárea, heridas de episiotomía, quemaduras, área do...

  9. Efeito do gel da babosa (Aloe barbadensis Mill. associado ao ultrassom em processo inflamatório agudo Effect of aloe (Aloe barbadensis Mill. gel combined with ultrasound in the acute inflammatory process

    Directory of Open Access Journals (Sweden)

    A.L.M Maia-Filho

    2011-01-01

    Full Text Available O presente trabalho teve por objetivo analisar a ação antiinflamatória do gel da Babosa a 2% (Aloe barbadensis Mill. associado ao Ultrassom pulsátil no modelo de edema de pata. Foram utilizados 25 ratos Wistar, (200-250 g, divididos em 5 grupos de 5 animais cada. Grupo1 (controle: ratos tratados com solução salina a 0,9%; Grupo 2: ratos tratados topicamente com gel de A. barbadensis Mill. a 2%; Grupo 3: animais tratados com Ultrassom; Grupo 4: ratos tratados com gel de A. barbadensis Mill. a 2% associado ao Ultrassom; Grupo 5 (controle positivo: ratos tratados com Indometacina na dose de 5 mg Kg-1. Os animais dos grupos 1 e 5 receberam os respectivos tratamentos por via intra-peritoneal 30 minutos antes da injeção intra-plantar de carragenina e os grupos 2, 3 e 4 foram tratados por aplicação tópica de gel de A. barbadensis Mill. a 2%, Ultrassom pulsátil e gel de A. barbadensis Mill. associado ao Ultrassom respectivamente 15 minutos após a indução do edema. Os animais do grupo 04 demonstraram redução significativa do edema quando comparados ao grupo controle, ao mesmo tempo, que se mostrou comparável à indometacina. Observou-se que o gel de aloe associado à fonoforose é capaz reduzir a formação do edema de pata em ratosThis work aimed to evaluate the anti-inflammatory action of 2% aloe (Aloe barbadensis Mill. gel combined with pulsed ultrasound in the paw edema model. Twenty-five Wistar rats (200-250 g were divided into 5 groups of 5 animals each. Group1 (control: rats treated with 0.9% saline; Group 2: rats topically treated with 2% aloe gel; Group 3: rats treated with ultrasound; Group 4: rats treated with 2% aloe gel combined with ultrasound; Group 5 (positive control: rats treated with indomethacin at 5 mg Kg-1. Animals of groups 1 and 5 were intraperitoneally treated 30 min before intraplantar carrageenan injection and groups 2, 3 and 4 were treated by topical application of 2% aloe gel, pulsed ultrasound and aloe gel

  10. Binding of the bioactive component Aloe dihydroisocoumarin with human serum albumin

    Science.gov (United States)

    Zhang, Xiu-Feng; Xie, Ling; Liu, Yang; Xiang, Jun-Feng; Tang, Ya-Lin

    2008-11-01

    Aloe dihydroisocoumarin, one of new components isolated from Aloe vera, can scavenge reactive oxygen species. In order to explore the mechanism of drug action at a molecular level, the binding of Aloe dihydroisocoumarin with human serum albumin (HSA) has been investigated by using fluorescence, ultraviolet (UV), circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy, fluorescence dynamics, and molecular dynamic docking for the first time. We observed a quenching of fluorescence of HSA in the presence of Aloe dihydroisocoumarin and also analyzed the quenching results using the Stern-Volmer equation and obtained high affinity binding to HSA. An isoemissive point at 414 nm is seen, indicating that the quenching of HSA fluorescence depends on the formation of Aloe dihydroisocoumarin-HSA complex, which is further confirmed by fluorescence dynamic result. From the CD and FT-IR results, it is apparent that the interaction of Aloe dihydroisocoumarin with HSA causes a conformational change of the protein, with the gain of α-helix, β-sheet and random coil stability and the loss of β-turn content. Data obtained by fluorescence spectroscopy, fluorescence dynamics, CD, and FTIR experiments along with the docking studies suggest that Aloe dihydroisocoumarin binds to residues located in subdomain IIA of HSA.

  11. Quality change of apple slices coated with Aloe vera gel during storage.

    Science.gov (United States)

    Song, Hye-Yeon; Jo, Wan-Shin; Song, Nak-Bum; Min, Sea C; Song, Kyung Bin

    2013-06-01

    Fresh-cut apples are easily susceptible to browning and microbial spoilage. In this study, an edible coating prepared from Aloe vera gel containing antibrowning solution was applied to preserve the quality of fresh-cut apples during storage. Fresh-cut apples were treated with both an Aloe vera gel and an Aloe vera gel containing 0.5% cysteine and then stored at 4 °C for 16 d. The color, firmness, weight loss, soluble solid content, titratable acidity, microbial analysis, and sensory evaluation were analyzed during storage. Fresh-cut apples coated with the Aloe vera gel showed delayed browning and reduced weight loss and softening compared to the control. The Aloe vera gel coating was also effective in reducing the populations of the total aerobic bacteria and yeast and molds. In particular, Aloe vera gel containing 0.5% cysteine was most effective in delaying browning and the reduction of microbial populations among the treatments. These results suggest that an Aloe vera gel coating can be used for maintaining the quality of fresh-cut apples. © 2013 Institute of Food Technologists®

  12. The effect of aloe vera on ischemia--Reperfusion injury of sciatic nerve in rats.

    Science.gov (United States)

    Guven, Mustafa; Gölge, Umut Hatay; Aslan, Esra; Sehitoglu, Muserref Hilal; Aras, Adem Bozkurt; Akman, Tarik; Cosar, Murat

    2016-04-01

    Aloe vera is compound which has strong antioxidant and anti-inflammatory effects. We investigated the neuroprotective role of aloe vera treatment in rats with experimental sciatic nerve ischemia/reperfusion injury. Twenty-eight male Wistar Albino rats were divided equally into 4 groups. Groups; Control group (no surgical procedure or medication), sciatic nerve ischemia/reperfusion group, sciatic nerve ischemia/reperfusion+aloe vera group and sciatic nerve ischemia/reperfusion+methylprednisolone group. Ischemia was performed by clamping the infrarenal abdominal aorta. 24 hours after ischemia, all animals were sacrificed. Sciatic nerve tissues were also examined histopathologically and biochemically. Ischemic fiber degeneration significantly decreased in the pre-treated with aloe vera and treated with methylprednisolone groups, especially in the pre-treated with aloe vera group, compared to the sciatic nerve ischemia/reperfusion group (paloe vera group was not statistically different compared to the MP group (p>0.05). Aloe vera is effective neuroprotective against sciatic nerve ischemia/reperfusion injury via antioxidant and anti-inflammatory properties. Also aloe vera was found to be as effective as MP. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  13. Effect of Aloe vera mouthwash on periodontal health: triple blind randomized control trial.

    Science.gov (United States)

    Karim, Bushra; Bhaskar, Dara John; Agali, Chandan; Gupta, Devanand; Gupta, Rajendra Kumar; Jain, Ankita; Kanwar, Alpana

    2014-03-01

    With the increasing incidence of periodontal diseases and development of antibiotic resistance, the global need for alternative treatment modalities, safe, effective, and economical products is the need of time. Aloe vera is a medicinal plant which has the greater medicinal value and enormous properties for curing and preventing oral diseases disease. The aim of the study was to access the effect of Aloe vera mouthwash on the dental plaque and gingivitis and comparing it with the bench mark control chlorhexidine and placebo. 345 healthy subjects were randomly allocated in 3 groups to the test group (n=115) - mouthwash containing Aloe vera, Control group (n=115) -chlorhexidene group, Distilled water - Placebo (n=115) . Plaque Index (PI) and Gingival Index (GI) were assessed at days 0, 15 and 30. Subjects were asked to rinse their mouth with the stated mouthwash, twice a day, during a 30-day period. Our result showed that Aloe vera mouthrinse is equally effective in reducing periodontal indices as Chlorhexidine. The results demonstrated a significant reduction of gingival bleeding and plaque indices in both groups over a period of 15 and 30 days as compared to placebo group. There was a significant reduction on plaque and gingivitis in Aloe vera and chlorhexidine groups and no statistically significant difference was observed among them (p>0.05). Aloe vera mouthwash showed no side effects as seen with chlorhexidine. The results of the present study indicate that Aloe vera may prove to be an effective mouthwash owing to its ability in reducing periodontal indices.

  14. In vitro studies on the photobiological properties of aloe emodin and aloin A.

    Science.gov (United States)

    Wamer, Wayne G; Vath, Peter; Falvey, Daniel E

    2003-01-15

    Plants containing aloin A, aloe emodin, and structurally related anthraquinones have long been used as traditional medicines and in the formulation of retail products such as laxatives, dietary supplements, and cosmetics. Since a recent study indicated that topically applied aloe emodin increases the sensitivity of skin to UV light, we examined the events following photoexcitation of aloin A and aloe emodin. We determined that incubation of human skin fibroblasts with 20 microM aloe emodin for 18 h followed by irradiation with UV or visible light resulted in significant photocytotoxicity. This photocytotoxicity was accompanied by oxidative damage in both cellular DNA and RNA. In contrast, no photocytotoxicity was observed following incubation with up to 500 microM aloin A and irradiation with UVA light. In an attempt to explain the different photobiological properties of aloin A and aloe emodin, laser flash photolysis experiments were performed. We determined that the triplet state of aloe emodin was readily formed following photoexcitation. However, no transient intermediates were formed following photoexcitation of aloin A. Therefore, generation of reactive oxygen species and oxidative damage after irradiation of aloin A is unlikely. Although aloin A was not directly photocytotoxic, we found that human skin fibroblasts can metabolize aloin A to aloe emodin.

  15. ALOE VERA JUICE DECREASE THE AMOUNT OF BLOOD GLUCOSE LEVEL IN PATIENT WITH DIABETIC TYPE II

    Directory of Open Access Journals (Sweden)

    Kusnanto Kusnanto

    2017-07-01

    Full Text Available Introduction: Aloe vera is a tropical plant that known can decrease the amount of blood glucose level in patient with diabetic type II. Diabetes mellitus is a disease cause by an increasing amount of blood glucose level that is reduce by conditions of the insulin. The aimed of this study was to analyze the effect of Aloe vera administering on decreasing blood glucose level for Diabetes mellitus type II patient. Method: A quasy experimental non randomized control group pre post test design was used in this study. There were 20 respondents divided into 3 group of treatment. Each group is given 100 gr/day 200 gr/day and 300 gr/day Aloe vera juice respectively. Data were collected by using observation and analyzed by using One way Anova and Paired t-Test with significance level α≤ 0.005. Result: The result showed that Aloe vera juice administering can decrease blood glucose level, with 100 gr/day, 200 gr/day, 300 gr/day (p=0.000.  Discussion: It can be proven that Aloe vera juice administering can decrease blood glucose level, it’s because the content of Aloe vera more complete and effect from alprogen which impede absorption glucose and blood glucose level. Further study are recommended to measure the effect of Aloe vera juice on blood glucose level with more consider on gender, age, total calorie consumption, obesity, activities, and stress.

  16. GONÇALO M. TAVARES’S POETIC MATERIALITY

    Directory of Open Access Journals (Sweden)

    Paloma Roriz Espínola

    2015-11-01

    Full Text Available It is not rare that arquitecture opens up to dialog with literature, like it is clearly noticed with the appropriation that some writers have done with it. Such as with Paul Valery’s and João Cabral de Melo Neto’s with their recria­tion of Amphion’s myth. The anglo-portuguese writer Gonçalo M. Tavares argues on two essays, Arquitetura, natureza e amor and A estranha casa do senhor Walser, an approximation of the duties of a writer and of an arqui­tect. The purpose of this essay is to demonstrate how Gonçalo M. Tavares’ approach reflects on his body of work which is based on the search of the material aspect of language. More especifically on the poems that compose his book entitled 1, a body of work which attempts to articulate ideas with the “emotional lucidity” of Roland Barthes and the “motion” of Georges Didi-Huberman.

  17. In vitro evaluation of the cytotoxic and apoptogenic properties of aloe whole leaf and gel materials.

    Science.gov (United States)

    du Plessis, Lissinda H; Hamman, Josias H

    2014-04-01

    Aloe gel and whole-leaf materials have shown biological effects with potential therapeutic applications, and recently, their drug-absorption enhancement properties have been discovered. It is important to establish a safety profile for these materials before they can be used in pharmaceutical products. The aim of the study was to investigate the in vitro cytotoxicity of Aloe vera, Aloe marlothii, Aloe speciosa and Aloe ferox against human hepatocellular (HepG2), human neuroblastoma cells (SH-SY5Y) and human adenocarcinoma epithelial cells (HeLa). Flow cytometry was used to measure cell viability, apoptosis and reactive oxygen species (ROS). The aloe gel materials investigated only decreased cell viability at concentrations of >10 mg/mL and exhibited half-maximal cytotoxic concentration (CC(50)) values above 1000 mg/mL, except for A. vera gel in HepG2 cells (CC(50) = 269.3 mg/mL). A. speciosa whole-leaf material showed a significant decrease in viability of Hela cells, whereas the other whole-leaf materials did not show a similar effect. The aloe gel materials in general showed low levels of apoptosis, whereas A. vera and A. speciosa whole-leaf materials caused a dose-dependent increase of apoptosis in HeLa cells. None of the aloe materials investigated exhibited a significant increase in ROS. It can be concluded that the selected aloe materials caused only limited reduction in cell viability with limited in vitro cytotoxicity effects. Further, neither significant apoptosis effects were observed nor induction of ROS.

  18. The Review on Properties of Aloe Vera in Healing of Cutaneous Wounds

    Science.gov (United States)

    Hashemi, Seyyed Abbas; Madani, Seyyed Abdollah; Abediankenari, Saied

    2015-01-01

    Treatment of wounds is very important and was subject of different investigations. In this regard, natural substance plays crucial role as complementary medicine. Various studies reported that aloe vera has useful effects on wounds especially cutaneous wounds healing. Therefore in the current review, we examined the effect of aloe vera on cutaneous wound healing and concluded that although aloe vera improves the wound healing as well as other procedures both clinically and experimentally, more studies are still needed to approve the outcomes. PMID:26090436

  19. The role of Aloe vera in various fields of medicine and dentistry

    Directory of Open Access Journals (Sweden)

    Andey Venkata Subhash

    2014-01-01

    Full Text Available Aloe vera is a cactus like plant that actually is part of the lily family. There are more than 300 varieties of the Aloe plant, but the Aloe barbadensis variety exhibits the best medicinal properties. It has strong antiseptic, antibacterial, fungicidal and virucidal properties. It promotes cell growth and is neurologically calming and acts as a detoxifying agent. Others provide nutritional support and some increase the regenerative potential of tissues while others act with anti-inflammatory responses. This article reviews the uses of the plant in different fields of medicine and dentistry.

  20. The Effect of Aloe, Gelfoam, Plaster on Bone Formation in applying to the bone defect

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Eui Hwan [Dept. of Oral and Maxillofacial Radiology, College of Dentistry, Chosun University and Oral Biology Research Center, Kwangju (Korea, Republic of); Kim, Su Gwan [Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University and Oral Biology Research Center, Kwangju (Korea, Republic of)

    1999-08-15

    This study was to evaluate the effects of Aloe, Gelfoam, and Plaster of Paris on bone healing. Four experimental defects were created for placement of the three materials in the right femur of dogs. One defect served as an empty control site. The evaluation was performed at 1-, 6-, and 12-weeks by light microscopy and NIH image program. Radiographic and Histologic examinations showed new bone formation in the presence of Aloe, Gelfoam, and Plaster of Paris and similar bone healing reactions. On the basis of these findings, it was concluded that Aloe, Gelfoam, and Plaster of Paris may be adequate agents for use in bone procurement.

  1. Aloe vera and garlic ameliorate thermoxidized palm oil-induced haemostatic derangement in albino Wistar rats

    OpenAIRE

    Ime, A. U.; Ani, E. J.; Nna, V. U.; Obeten, C. E.

    2017-01-01

    This study examined the effects of Aloe vera and garlic on haemostatic status of rats fed thermoxidized palm oil diet (TPO). 35 male Wistar rats (140-170 grams) used in this study were randomly assigned five groups (n=7): Control, TPO, TPO + garlic juice (TPO+G), TPO + Aloe gel (TPO+A) and TPO + garlic/Aloe gel (TPO+G+A). TPO diet was prepared by mixing 85 g of rat chow with 15 g of thermoxidized oil. The juice and gel were orally administered at doses of 2 ml/kg and 6 ml/kg respectively. Aft...

  2. Antidiabetic activity of Aloe vera L. juice. I. Clinical trial in new cases of diabetes mellitus.

    Science.gov (United States)

    Yongchaiyudha, S; Rungpitarangsi, V; Bunyapraphatsara, N; Chokechaijaroenporn, O

    1996-11-01

    Aloe vera gel has been claimed to have antidiabetic activity but not all published results are consistent. We investigated the effect of oral administration of one tablespoonful of Aloe vera juice, twice a day for at least 2 weeks in patients with diabetes. Blood sugar and triglyceride levels in the treated group fell; cholesterol levels were not affected. The results suggest the potential of aloe vera juice for use as an antidiabetic agent. Copyright © 1996 Gustav Fischer Verlag · Stuttgart · Jena · New York. Published by Elsevier GmbH.. All rights reserved.

  3. Karakteristik Fisik, Kimia, Dan Sifat Organoleptik Yoghurt Dengan Campuran Berbagai Konsentrasi Sari Lidah Buaya (Aloe Vera)

    OpenAIRE

    Adriyan, -; Aminah, Siti

    2012-01-01

    Yoghurt is a beverage product derived from cow's milk that has a slightly sour taste as fermented by Lactic Acid Bacteria (LAB). Aloe vera has a content of nutrients including calcium, potassium, magnesium, vitamin A, B1, B2, B6, B complex, vitamin C, vitamin E, and protein. Need to develop into juice of aloe vera as a material added in yoghurt. The aim of study are test of pH, viscosity, total acid, fiber, and the organoleptic properties of aloe vera yoghurt. The method used is experiment wi...

  4. Aloe emodin, an anthroquinone from Aloe vera acts as an anti aggregatory agent to the thermally aggregated hemoglobin

    Science.gov (United States)

    Furkan, Mohammad; Alam, Md Tauqir; Rizvi, Asim; Khan, Kashan; Ali, Abad; Shamsuzzaman; Naeem, Aabgeena

    2017-05-01

    Aggregation of proteins is a physiological process which contributes to the pathophysiology of several maladies including diabetes mellitus, Huntington's and Alzheimer's disease. In this study we have reported that aloe emodin (AE), an anthroquinone, which is one of the active components of the Aloe vera plant, acts as an inhibitor of hemoglobin (Hb) aggregation. Hb was thermally aggregated at 60 °C for four days as evident by increased thioflavin T and ANS fluorescence, shifted congo red absorbance, appearance of β sheet structure, increase in turbidity and presence of oligomeric aggregates. Increasing concentration of AE partially reverses the aggregation of the model heme protein (hemoglobin). The maximum effect of AE was observed at 100 μM followed by saturation at 125 μM. The results were confirmed by UV-visible spectrometry, intrinsic fluorescence, ThT, ANS, congo red assay as well as transmission electron microscopy (TEM). These results were also supported by fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD) which shows the disappearance of β sheet structure and appearance of α helices. This study will serve as baseline for translatory research and the development of AE based therapeutics for diseases attributed to protein aggregation.

  5. Antiviral activity of the crude extracts and phytochemical fractions of ...

    African Journals Online (AJOL)

    The three fractions used contained the major peaks within which the main compounds had been identified as aloenin, aloin and an aloinoside derivatives. The crude Aloe extract at 400 mg/ml exhibited antiviral effects at 100%, While 200 mg/ml resulted to 30% reduction in viral multiplication. Fraction containing aloenin (4 ...

  6. Inactivation of enveloped viruses by anthraquinones extracted from plants.

    Science.gov (United States)

    Sydiskis, R J; Owen, D G; Lohr, J L; Rosler, K H; Blomster, R N

    1991-01-01

    To determine the extent of antiviral activity present in a number of plant extracts, hot glycerin extracts were prepared from Rheum officinale, Aloe barbadensis, Rhamnus frangula, Rhamnus purshianus, and Cassia angustifolia and their virucidal effects were tested against herpes simplex virus type 1. All the plant extracts inactivated the virus. The active components in these plants were separated by thin-layer chromatography and identified as anthraquinones. A purified sample of aloe emodin was prepared from aloin, and its effects on the infectivity of herpes simplex virus type 1 and type 2, varicella-zoster virus, pseudorabies virus, influenza virus, adenovirus, and rhinovirus were tested by mixing virus with dilutions of aloe emodin for 15 min at 37 degrees C, immediately diluting the sample, and assaying the amount of infectious virus remaining in the sample. The results showed that aloe emodin inactivated all of the viruses tested except adenovirus and rhinovirus. Electron microscopic examination of anthraquinone-treated herpes simplex virus demonstrated that the envelopes were partially disrupted. These results show that anthraquinones extracted from a variety of plants are directly virucidal to enveloped viruses. PMID:1810179

  7. Analysis of traumatic ulcer healing time under the treatment of the Mauli banana (Musa acuminata 25% stem extract gel

    Directory of Open Access Journals (Sweden)

    Dewi Puspitasari

    2017-03-01

    Full Text Available ABSTRACT Introduction: Traumatic ulcer is one of oral disorders that often occur. The prevalence is quite high, between 3-24% of population. Therapy of traumatic ulcer is symptomatic. People usually use Aloe vera extract gel. Previous research showed that the Aloe vera gel is equivalent with ethanol extract of 25% Mauli banana (Musa acuminata stem in accelerating wound healing based on the number of macrophages and neovascular. The objective of the study is to find out the time difference of traumatic ulcer healing using 25% Musa acuminata stem extract gel compared to a gel containing Aloe vera extract. Methods: The research was post test design. Subjects were patients of Oral Medicine Installation in Gusti Hasan Aman Dental Hospital Banjarmasin with diagnosis of traumatic ulcer using completely random sampling method. Eight patients as samples were determined by the formula of Lemeshow. Results: there was no significant difference with p=0,724 (p>0,05 between healing time using Aloe vera gel and 25% Musa acuminata stem extract. Conclusion: At the concentration of 25% Musa acuminata stem extract gel concentration has the same healing time with Aloe vera gel, therefore can be recommended as a topical use for traumatic ulcer healing. Keywords: Musa acuminata stem, Healing time, Traumatic ulcer

  8. Natural phosphodiesterase-4 inhibitors from the leaf skin of Aloe barbadensis Miller.

    Science.gov (United States)

    Zhong, Jia-Sheng; Huang, Yi-You; Zhang, Tian-Hua; Liu, Yu-Peng; Ding, Wen-Jing; Wu, Xiao-Fang; Xie, Zhi-Yong; Luo, Hai-Bin; Wan, Jin-Zhi

    2015-01-01

    The ethanolic extract of Aloe barbadensis Miller leaf skin showed inhibitory activity against phosphodiesterase-4D (PDE4D), which is a therapeutic target of inflammatory disease. Subsequent bioassay-guided fractionation led to the isolation of two new anthrones, 6'-O-acetyl-aloin B (9) and 6'-O-acetyl-aloin A (11), one new chromone, aloeresin K (8), together with thirteen known compounds. Their chemical structures were elucidated by spectroscopic methods including UV, IR, 1D and 2D NMR, and HRMS. All of the isolates were screened for their inhibitory activity against PDE4D using tritium-labeled adenosine 3',5'-cyclic monophosphate ((3)H-cAMP) as substrate. Compounds 13 and 14 were identified as PDE4D inhibitors, with their IC50 values of 9.25 and 4.42 μM, respectively. These achievements can provide evidences for the use of A. barbadensis leaf skin as functional feed additives for anti-inflammatory purpose. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. Making SAR Data Accessible - ASF's ALOS PALSAR Radiometric Terrain Correction Project

    Science.gov (United States)

    Meyer, F. J.; Arko, S. A.; Gens, R.

    2015-12-01

    While SAR data have proven valuable for a wide range of geophysical research questions, so far, largely only the SAR-educated science communities have been able to fully exploit the information content of internationally available SAR archives. The main issues that have been preventing a more widespread utilization of SAR are related to (1) the diversity and complexity of SAR data formats, (2) the complexity of the processing flows needed to extract geophysical information from SAR, (3) the lack of standardization and automation of these processing flows, and (4) the often ignored geocoding procedures, leaving the data in image coordinate space. In order to improve upon this situation, ASF's radiometric terrain-correction (RTC) project is generating uniformly formatted and easily accessible value-added products from the ASF Distributed Active Archive Center's (DAAC) five-year archive of JAXA's ALOS PALSAR sensor. Specifically, the project applies geometric and radiometric corrections to SAR data to allow for an easy and direct combination of obliquely acquired SAR data with remote sensing imagery acquired in nadir observation geometries. Finally, the value-added data is provided to the user in the broadly accepted Geotiff format, in order to support the easy integration of SAR data into GIS environments. The goal of ASF's RTC project is to make SAR data more accessible and more attractive to the broader SAR applications community, especially to those users that currently have limited SAR expertise. Production of RTC products commenced October 2014 and will conclude late in 2015. As of July 2015, processing of 71% of ASF's ALOS PALSAR archive was completed. Adding to the utility of this dataset are recent changes to the data access policy that allow the full-resolution RTC products to be provided to the public, without restriction. In this paper we will introduce the processing flow that was developed for the RTC project and summarize the calibration and validation

  10. Detecting and Mapping Invasive Phragmites australis in the coastal Great Lakes with ALOS PALSAR imagery

    Science.gov (United States)

    Bourgeau-chavez, L. L.; Scarbrough, K.; Jenkins, L. K.; Riordan, K.; Powell, R. B.; Brooks, C.; Kowalski, K.; Carlson Mazur, M.; Huberty, B.

    2011-12-01

    Phragmites australis is a non-native invasive plant that can form dense monocultures, causing negative impacts on coastal Great Lakes wetlands by reducing ecosystem services including habitat and therefore, biological diversity. Through Great Lakes Restoration Initiative funding, ALOS PALSAR imagery is being used to map the invasive plant as it occurs in monoculture stands of the U.S. coastal Great Lakes wetlands. These invasive Phragmites maps are being used as part of a USGS Great Lakes Science Center (GLSC) and US Fish and Wildlife Service (USFWS) National Wetlands Inventory (NWI) program to identify major environmental drivers of invasive Phragmites distribution, to assess areas vulnerable to new invasion, and to provide this information to regional stakeholders through a decision support tool. The invasive Phragmites map is the first U.S. basin-wide map to be produced on the distribution of this species. Methods include maximum likelihood classification of multi-season ALOS PALSAR HH and HV polarization data. PALSAR is an L-band (23 cm wavelength) imaging radar sensor which is sensitive to differences in plant biomass and inundation patterns, allowing for the extraction of these tall (up to 15 m), high-density, high-biomass Phragmites wetland stands. To improve discrimination of Phragmites australis, the three date (spring, summer, fall) dataset is being used, which takes advantage of phenological changes in vegetation and inundation patterns over the seasons. Field collections of training and randomly selected validation data were conducted in spring summer and fall of 2010-11 to aid in the mapping and for accuracy assessment. The minimum mapping unit is 1/2 acre and thus all field sites were sampled at 1/2 acre units. All map products and field validation data will be complete by December 2011. Maps are being completed on a Lake basin basis. The first final map product was delivered for Lake Erie coastal wetlands to 10 km inland, with an overall map accuracy

  11. Influence de l'extrait aquueux de feuilles d'Aloe buettneri, Dicliptera ...

    African Journals Online (AJOL)

    Influence de l'extrait aquueux de feuilles d'Aloe buettneri, Dicliptera verticillata, Hibiscus macranthus et Justicia insularis sur la fertilité et quelques parametres biochemiques de la reproduction chez la rate.

  12. Comparative evaluation of natural antioxidants spirulina and aloe vera for the treatment of oral submucous fibrosis.

    Science.gov (United States)

    Patil, Santosh; Al-Zarea, Bader Kureyem; Maheshwari, Sneha; Sahu, Rohit

    2015-01-01

    Oral submucous fibrosis (OSMF) is a high risk premalignant condition predominantly seen in the Indian subcontinent. The aim of the present study was to compare the efficacy of spirulina and aloe vera in the management of OSMF. 42 subjects with clinico-pathologically diagnosed OSMF were included in the study and divided equally in 2 groups, Group A (spirulina group) and Group B (aloe vera group). Group A was administered 500 mg spirulina in 2 divided doses for 3 months and Group B was given 5 mg aloe vera gel to be applied topically thrice daily for 3 months. Evaluation for different clinical parameters was done at regular intervals and data was analyzed using the Chi-square test. P-value aloe vera for the treatment of OSMF.

  13. Comparative efficacy of aloe vera mouthwash and chlorhexidine on periodontal health: A randomized controlled trial.

    Science.gov (United States)

    Vangipuram, Swathi; Jha, Abhishek; Bhashyam, Mamtha

    2016-10-01

    With introduction of many herbal medicines, dentistry has recently evidenced shift of approach for treating many inflammatory oral diseases by using such modalities. Aloe vera is one such product exhibiting multiple benefits and has gained considerable importance in clinical research recently. To compare the efficacy of Aloevera and Chlorhexidine mouthwash on Periodontal Health. Thirty days randomized controlled trial was conducted among 390 dental students. The students were randomized into two intervention groups namely Aloe Vera (AV) chlorhexidine group (CHX) and one control (placebo) group. Plaque index and gingival index was recorded for each participant at baseline, 15 days and 30 days. The findings were than statistically analyzed, ANOVA and Post Hoc test were used. There was significant reduction (pAloe Vera (AV) and chlorhexidine group. Post hoc test showed significant difference (paloe Vera and placebo and chlorhexidine and placebo group. No significant difference (pAloe vera, chlorhexidine, dental plaque, gingivitis.

  14. Theoretical investigation of the long-lived metastable AlO2+ dication in gas phase

    Science.gov (United States)

    Sghaier, Onsi; Abdallah, Hassan H.; Abdullah, Hewa Y.; Jaidane, Nejm Eddine; Al Mogren, Muneerah Mogren; Hochlaf, Majdi

    2016-09-01

    We report the results of a detailed theoretical study of the electronic ground and excited states of the gas-phase doubly charged ion AlO2+ using high-level ab initio computer calculations. Both standard and explicitly correlated methods were used to calculate their potential energy curves and spectroscopic parameters. These computations show that the ground state of AlO2+ is X2Π. The internuclear equilibrium distance of AlO2+(X2Π) is computed 1.725 Å. We also deduced the adiabatic double ionization and charge stripping energies of AlO to be about 27.45 eV and 17.80 eV, respectively.

  15. Aloe vera in active and passive regions of electronic devices towards a sustainable development

    Science.gov (United States)

    Lim, Zhe Xi; Sreenivasan, Sasidharan; Wong, Yew Hoong; Cheong, Kuan Yew

    2017-07-01

    The increasing awareness towards sustainable development of electronics has driven the search for natural bio-organic materials in place of conventional electronic materials. The concept of using natural bio-organic materials in electronics provides not only an effective solution to address global electronic waste crisis, but also a compelling template for sustainable electronics manufacturing. This paper attempts to provide an overview of using Aloe vera gel as a natural bio-organic material for various electronic applications. Important concepts such as responses of living Aloe vera plant towards electrical stimuli and demonstrations of Aloe vera films as passive and active regions of electronic devices are highlighted in chronological order. The biodegradability and biocompatibility of Aloe vera can bring the world a step closer towards the ultimate goal of sustainable development of electronic devices from "all-natural" materials.

  16. The combined effects of Aloe vera gel and silver nanoparticles on wound healing in rats

    Directory of Open Access Journals (Sweden)

    Y. Yousefpoor

    2016-01-01

    Full Text Available Objective(s: This study was aimed at investigating the synergy effects of Aloe vera gel and silver nanoparticles on the healing rate of the cutting wounds. Materials and Methods: In order to determine the concentration of silver nanoparticles in Aloe vera gel, the MBC methods were applied on the most common bacteria infecting wounds, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The cutting wounds with Full-thickness skin were dorsally created on rats; then the rats were divided into 4 groups. The treatments groups included: mixture of Aloe vera gel and silver nanoparticles, Aloe vera gel alone and silver nanoparticles alone in addition to control groups. The treatment was carried out for 2 weeks and the size of the wound closures were measured by an image software analysis. Results:There was no significant difference (p

  17. Effect of Aloe vera juice on growth and activities of Lactobacilli in-vitro

    National Research Council Canada - National Science Library

    Nagpal, Ravinder; Kaur, Varinder; Kumar, Manoj; Marotta, Francesco

    2012-01-01

    In present investigation, different concentrations of Aloe vera juice incorporated into the growth media of Lactobacilli were tested to observe the effect on growth and activities of these bacteria...

  18. In vivo antitrypanosomal effects of stem-bark extracts of Securidaca ...

    African Journals Online (AJOL)

    ADEYEYE

    2017-07-14

    Jul 14, 2017 ... groups 1 and 2 received the crude methanol extract (CME) at 0.7 and 0.35 mg/kg, respectively. Similarly .... S. longipedunculata extract and fractions were ..... Aloe vera. Pharmacognosy Communications, 1(1): 52-. 62. Cordell GA, Quinn-Beattie ML & Farnsworth NR. (2001). The potential of alkaloids in drug.

  19. A review on ethnopharmacological potential of Aloe vera L.

    Directory of Open Access Journals (Sweden)

    Md. Shamim Hossain

    2013-04-01

    Full Text Available In recent years, Aloe vera Linn. (Ghritokumari locally has become a subject of interest because of its beneficial effects on human health. The present ethnopharmacological review was conducted to evaluate the therapeutic properties of A. vera by scientific evidences. It belongs to the family Liliaceae, is a perennial herb with 30-60cm long juicy leaves which is found all over Bangladesh. To date, more than 75 active ingredients including aloesin, aloeemodin, acemannan, aloeride, methylchromones, flavonoids, saponin, amino acids, vitamins, and minerals have been identified from inner gel of leaves. It has antiinflammatory, antioxidant, antimicrobial, anticancer, antidiabetic, immuneboosting, and hypoglycemic properties. Daily supplementation with this is effective against stroke, heart attacks, leukemia, anemia, hypertension, AIDS, radiation burns, digestive disorders etc. This study also covers its taxonomy, distribution, morphology, and monograph. [J Intercult Ethnopharmacol 2013; 2(2.000: 113-120

  20. Tyrosinase inhibitory components from Aloe vera and their antiviral activity.

    Science.gov (United States)

    Kim, Jang Hoon; Yoon, Ju-Yeon; Yang, Seo Young; Choi, Seung-Kook; Kwon, Sun Jung; Cho, In Sook; Jeong, Min Hee; Ho Kim, Young; Choi, Gug Seoun

    2017-12-01

    A new compound, 9-dihydroxyl-2'-O-(Z)-cinnamoyl-7-methoxy-aloesin (1), and eight known compounds (2-9) were isolated from Aloe vera. Their structures were elucidated using 1D/2D nuclear magnetic resonance and mass spectra. Compound 9 exhibited reversible competitive inhibitory activity against the enzyme tyrosinase, with an IC50 value of 9.8 ± 0.9 µM. A molecular simulation revealed that compound 9 interacts via hydrogen bonding with residues His244, Thr261, and Val283 of tyrosinase. Additionally, compounds 3 and 7 were shown by half-leaf assays to exhibit inhibitory activity towards Pepper mild mottle virus.

  1. Aloe vera: an in vitro study of effects on corneal wound closure and collagenase activity.

    Science.gov (United States)

    Curto, Elizabeth M; Labelle, Amber; Chandler, Heather L

    2014-11-01

    To evaluate the in vitro effects of an aloe vera solution on (i) the viability and wound healing response of corneal cells and (ii) the ability to alter collagenase and gelatinase activities. Primary cultures of corneal epithelial cells and fibroblasts were prepared from grossly normal enucleated canine globes and treated with an aloe solution (doses ranging from 0.0-2 mg/mL). Cellular viability was evaluated using a colorimetric assay. A corneal wound healing model was used to quantify cellular ingrowth across a defect made on the confluent surface. Anticollagenase and antigelatinase activities were evaluated by incubating a bacterial collagenase/gelatinase with aloe solution (doses ranging from 0.0-500 μg/mL) and comparing outcome measures to a general metalloproteinase inhibitor, 1, 10-phenanthroline, and canine serum (doses ranging from 0.0-100%). None of the concentrations of aloe solution tested significantly affected the viability of corneal epithelial cells or fibroblasts. Concentrations ≤175 μg/mL slightly accelerated corneal epithelial cell wound closure; this change was not significant. Concentrations ≥175 μg/mL significantly (P ≤ 0.001) slowed the rate of corneal fibroblast wound closure, while aloe concentrations Aloe solution did not alter the ability for collagenase to degrade gelatin or collagen Type I but increased the ability for collagenase to degrade Type IV collagen. Although additional experiments are required, lower concentrations of aloe solution may be beneficial in healing of superficial corneal wounds to help decrease fibrosis and speed epithelialization. An increase in collagenase activity with aloe vera warrants further testing before considering in vivo studies. © 2014 American College of Veterinary Ophthalmologists.

  2. Effectiveness of Aloe Vera Gel in Chronic Ulcers in Comparison with Conventional Treatments.

    Science.gov (United States)

    Avijgan, Majid; Kamran, Asghar; Abedini, Amin

    2016-05-01

    Aloe Vera is one of the endemic plants in southern Iran, which has been mentioned in the textbooks of Persian medicine since 2500 years ago. The aim of this study was to compare the effectiveness and cost of Aloe Vera gel with conventional treatments in patients with chronic ulcers. This comparative study was conducted on 60 patients with chronic ulcers (more than 3 weeks) in Al-Zahra hospital (Isfahan, Iran) in 2015. The participants were divided into two groups of 30 patients per group. In one group, we used conventional treatment plus Aloe Vera gel and in the other group, only the conventional treatment was used. In the Aloe Vera group, we used Aloe Vera gel twice a day. The patients were followed-up a week after the treatment and then monthly for 3 months. The male: female ratio was 1:1 in each group. The mean age of the Aloe Vera and control groups were 62.3±11.2 and 63.1±9.6, respectively. After three months follow-up, wound healing occurred in 28 (93.3%) patients in the Aloe Vera group and 14 (46.7%) patients in the control group (PAloe Vera and control groups, respectively (PAloe Vera and control groups, respectively (PAloe Vera gel and conventional treatment per patient was $2 and $10 daily, respectively (PAloe Vera gel is a beneficial treatment and cost effective for patients with chronic ulcers. The use of Aloe Vera gel in chronic ulcer is recommended in developing countries to lessen the financial burden.

  3. Aloe Vera Juice Decrease the Amount of Blood Glucose Level in Patient with Diabetic Type II

    OpenAIRE

    Kusnanto Kusnanto; Sriyono Sriyono; Dian Eko Puji Astuti

    2008-01-01

    Introduction: Aloe vera is a tropical plant that known can decrease the amount of blood glucose level in patient with diabetic type II. Diabetes mellitus is a disease cause by an increasing amount of blood glucose level that is reduce by conditions of the insulin. The aimed of this study was to analyze the effect of Aloe vera administering on decreasing blood glucose level for Diabetes mellitus type II patient. Method: A quasy experimental non randomized control group pre post test design was...

  4. SEBARAN ASPEK KERUANGAN TIPE LONGSORAN DI DAERAH ALIRAN SUNGAI ALO PROVINSI GORONTALO (Spread of Spatial Aspect of Landslide Types at Alo Watershed in Gorontalo Province

    Directory of Open Access Journals (Sweden)

    Fitryane Lihawa

    2015-01-01

    Full Text Available ABSTRAK Sebaran aspek keruangan tipe longsoran di DAS Alo Provinsi Gorontalo telah dikaji dan dievaluasi menggunakan bentuk Peta Sebaran Tipe Longsoran Skala 1 : 50.000. Lokasi penelitian ini meliputi seluruh wilayah DAS Alo Provinsi Gorontalo dengan luas 7.588 Ha. Penentuan sampel penelitian dilakukan secara Accidental Sampling yaitu dengan melakukan penelusuran di seluruh wilayah yang rawan longsor di DAS Alo untuk menemukan titik-titik kejadian longsoran. Dalam pengkajian tipe dan sebaran longsoran, dilakukan pengamatan dan pengukuran terhadap kejadian longsoran yang terjadi pada seluruh wilayah DAS Alo yaitu sejumlah 15 (lima belas titik kejadian longsoran.  Tipe longsoran ditentukan melalui pengukuran dan pengamatan morfometri longsoran untuk menentukan indeks klasifikasi longsoran dan hasil analisis tersebut di plot ke dalam Peta Lokasi Sebaran Tipe Longsoran Skala 1 : 50.000 untuk mengetahui sebaran keruangan dari kejadian longsoran di DAS Alo Provinsi Gorontalo. Berdasarkan analisis morfometri dan indeks klasifikasi longsoran menunjukkan bahwa tipe longsoran yang terjadi adalah rotational slide, planar slide, slide flow dan rock block slide. Kejadian longsoran yang terjadi di DAS Alo Provinsi Gorontalo tersebar pada wilayah dengan kemiringan lereng curam dan sangat curam dengan bentuk permukaan lereng cembung dan cenderung lurus. Kejadian longsoran juga terjadi pada wilayah dengan tekstur tanah lempung dan lempung berlanau, serta jenis batuan vulkanik dan batuan beku yang mengandung silika tinggi dan telah mengalami pelapukan. Berdasarkan wilayah  administrasi, kejadian longsoran tersebar di wilayah Kecamatan Tibawa, Kecamatan Pulubala dan Kecamatan Isimu Utara.    ABSTRACT Distribution of spatial aspect of landslide at ALO Watershed of Gorontalo Province has been studied by providing it through form of spread landslide maps at scale of 1 : 50.000. Research site involved all areas of ALO Watershed in Gorontalo Province as having an

  5. Release Behavior and Antibacterial Activity of Chitosan/Alginate Blends with Aloe vera and Silver Nanoparticles

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    Luisa Fernanda Gómez Chabala

    2017-10-01

    Full Text Available Aloe vera is a perennial plant employed for medical, pharmaceutical and cosmetic purposes that is rich in amino acids, enzymes, vitamins and polysaccharides, which are responsible for its therapeutic properties. Incorporating these properties into a biopolymer film obtained from alginate and chitosan allowed the development of a novel wound dressing with antibacterial capacity and healing effects to integrate the antibacterial capacity of silver nanoparticles with the healing and emollient properties of Aloe vera gel. Three alginate-chitosan matrices were obtained through blending methods using different proportions of alginate, chitosan, the Aloe vera (AV gel and silver nanoparticles (AgNps, which were incorporated into the polymeric system through immersion methods. Physical, chemical and antibacterial characteristics were evaluated in each matrix. Interaction between alginate and chitosan was identified using the Fourier transform infrared spectroscopy technique (FTIR, porosity was studied using scanning electron microscopy (SEM, swelling degree was calculated by difference in weight, Aloe vera gel release capacity was estimated by applying a drug model (Peppas and finally antibacterial capacity was evaluated against S. Aureus and P. aeruginosa. Results show that alginate-chitosan (A (1:3 Chit 1/Alg 1; B (1:3 Chit 1.5/Alg 1 and C (3:1 Chit 1/Alg 1/B12 matrices with Aloe vera (AV gel and silver nanoparticles (AgNps described here displayed antibacterial properties and absorption and Aloe vera release capacity making it a potential wound dressing for minor injuries.

  6. Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model.

    Science.gov (United States)

    Lin, Li-Xin; Wang, Peng; Wang, Yu-Ting; Huang, Yong; Jiang, Lei; Wang, Xue-Ming

    2016-02-01

    Aloe vera and Vitis vinifera have been traditionally used as wound healing agents. The present study aimed to investigate the effects of aloe emodin and resveratrol in the burn wound healing procedure. Burn wounds are common in developed and developing countries, however, in developing countries, the incidence of severe complications is higher and financial resources are limited. The results of the present study demonstrated that neither aloe emodin or resveratrol were cytotoxic to THP-1 macrophages at concentrations of 1, 100 and 500 ng/ml. A significant increase in wound-healing activity was observed in mice treated with the aloe emodin and resveratrol, compared with those which received control treatments. The levels of IL-1β in the exudates of the burn wound area of the treated mice increased in a time-dependent manner over 7 days following burn wound injury. At 10 days post-injury, steady and progressive wound healing was observed in the control animals. The present study confirmed that increased wound healing occurs following treatment with aloe emodin,, compared with resveratrol, providing support for the use of Aloe vera plants to improve burn wound healing.

  7. Aloe-emodin suppresses prostate cancer by targeting the mTOR complex 2

    Science.gov (United States)

    Liu, Kangdong; Park, Chanmi; Lee, Ki Won; Liu, Haidan; He, Long; Soung, Nak Kyun; Ahn, Jong Seog; Bode, Ann M.; Dong, Ziming; Dong, Zigang

    2012-01-01

    Phosphatidylinositol 3-kinase (PI3-K) amplification and phosphatase and tensin homolog (PTEN) deletion-caused Akt activation contribute to the development of prostate cancer. Mammalian target of rapamycin complex 2 (mTORC2) is a kinase complex comprised of mTOR, Rictor, mSin1, mLST8/GβL and PRR5 and functions in the phosphorylation of Akt at Ser473. Herein, we report that mTORC2 plays an important role in PC3 androgen refractory prostate cell proliferation and anchorage-independent growth. Aloe-emodin, a natural compound found in aloe, inhibited both proliferation and anchorage-independent growth of PC3 cells. Protein content analysis suggested that activation of the downstream substrates of mTORC2, Akt and PKCα, was inhibited by aloe-emodin treatment. Pull-down assay and in vitro kinase assay results indicated that aloe-emodin could bind with mTORC2 in cells and inhibit its kinase activity. Aloe-emodin also exhibited tumor suppression effects in vivo in an athymic nude mouse model. Collectively, our data suggest that mTORC2 plays an important role in prostate cancer development and aloe-emodin suppresses prostate cancer progression by targeting mTORC2. PMID:22532249

  8. Molecular interaction and energy transfer between human serum albumin and bioactive component Aloe dihydrocoumarin

    Science.gov (United States)

    Zhang, Xiu-Feng; Xie, Ling; Liu, Yang; Xiang, Jun-Feng; Li, Lin; Tang, Ya-Lin

    2008-10-01

    Aloe dihydrocoumarin is an antioxidant and a candidate of immunomodulatory drug on the immune system and can balance physiological reactive oxygen species (ROS) levels which may be useful to maintain homeostasis. In order to explore the mechanism of drug action at a molecular level, the binding of Aloe dihydrocoumarin with human serum albumin (HSA) has been investigated by fluorescence, ultraviolet (UV), circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy, fluorescence dynamics, and molecular dynamic docking for the first time. We observed a quenching of fluorescence of HSA in the presence of Aloe dihydrocoumarin and also analyzed the quenching results using the Stern-Volmer equation and obtained high affinity binding to HSA. A Förster type fluorescence resonance energy transfer mechanism is involved in this quenching of Trp fluorescence by Aloe dihydrocoumarin. From the CD and FT-IR results, it is apparent that the interaction of Aloe dihydrocoumarin with HSA causes a conformational change of the protein, with the loss of α-helix stability and the gain of β-sheet and β-turn content. Data obtained by fluorescence spectroscopy, fluorescence dynamics, CD, and FT-IR experiments along with the docking studies suggest that Aloe dihydrocoumarin binds to residues located in subdomain IIA of HSA.

  9. Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation.

    Science.gov (United States)

    Carvalho, Nayane Chagas; Guedes, Simone Alves Garcez; Albuquerque-Júnior, Ricardo Luiz Cavalcanti; de Albuquerque, Diana Santana; de Souza Araújo, Adriano Antunes; Paranhos, Luiz Renato; Camargo, Samira Esteves Afonso; Ribeiro, Maria Amália Gonzaga

    2018-01-01

    This study aims to evaluate, in vitro, the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled water; AL - Aloe vera with distilled water and FTL; HA - calcium hydroxide P.A. with distilled water; HL - calcium hydroxide P.A. with distilled water and FTL; HAA - calcium hydroxide P.A. with Aloe vera and distilled water; HAL - calcium hydroxide P.A. with Aloe vera, distilled water, and FTL. The cytotoxicity was evaluated by MTT assay at 24, 48, and 72h and the genotoxicity by micronucleus test assay. This study was performed in triplicate. Data obtained in both tests were statistically analyzed by ANOVA and Tukey's tests (p≤0.05). Group AA presented high genotoxicity and low cytotoxicity. After 24, 48, and 72h, the group HAA significantly reduced the cell viability. Interaction with FTL showed slightly increase cell viability after 24 and 48h in groups CL and HL (pAloe vera allowed higher cell viability in human pulp fibroblasts in the presence of calcium hydroxide or with FTL separately, but genotoxicity increased in these associations. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Release Behavior and Antibacterial Activity of Chitosan/Alginate Blends with Aloe vera and Silver Nanoparticles.

    Science.gov (United States)

    Gómez Chabala, Luisa Fernanda; Cuartas, Claudia Elena Echeverri; López, Martha Elena Londoño

    2017-10-24

    Aloe vera is a perennial plant employed for medical, pharmaceutical and cosmetic purposes that is rich in amino acids, enzymes, vitamins and polysaccharides, which are responsible for its therapeutic properties. Incorporating these properties into a biopolymer film obtained from alginate and chitosan allowed the development of a novel wound dressing with antibacterial capacity and healing effects to integrate the antibacterial capacity of silver nanoparticles with the healing and emollient properties of Aloe vera gel. Three alginate-chitosan matrices were obtained through blending methods using different proportions of alginate, chitosan, the Aloe vera (AV) gel and silver nanoparticles (AgNps), which were incorporated into the polymeric system through immersion methods. Physical, chemical and antibacterial characteristics were evaluated in each matrix. Interaction between alginate and chitosan was identified using the Fourier transform infrared spectroscopy technique (FTIR), porosity was studied using scanning electron microscopy (SEM), swelling degree was calculated by difference in weight, Aloe vera gel release capacity was estimated by applying a drug model (Peppas) and finally antibacterial capacity was evaluated against S. Aureus and P. aeruginosa. Results show that alginate-chitosan (A (1:3 Chit 1/Alg 1); B (1:3 Chit 1.5/Alg 1) and C (3:1 Chit 1/Alg 1/B12)) matrices with Aloe vera (AV) gel and silver nanoparticles (AgNps) described here displayed antibacterial properties and absorption and Aloe vera release capacity making it a potential wound dressing for minor injuries.

  11. Effectiveness of Aloe vera on the antioxidant status of different tissues in irradiated rats.

    Science.gov (United States)

    Saada, H N; Ussama, Z S; Mahdy, A M

    2003-12-01

    This study was performed to evaluate the role of Aloe vera (Aloe barbadensis Miller) on the antioxidant status in different tissues of animals whole body exposed to 7 Gy gamma radiations, delivered as a shot dose. Aloe vera (leaf juice filtrate) was supplemented daily to rats (0.25 ml/kg body weight/day), by gavage, 5 days before irradiation and 10 days after irradiation. Experimental investigations performed 3, 7 and 10 days after exposure to radiation showed that Aloe vera treatment has significantly minimized the radiation-induced increase in the amount of malondialdehyde in liver, lungs, and kidney tissues of irradiated rats. Significant amelioration in superoxide dismutase (SOD) and catalase activities was observed from the 3rd up to the 10th days for lungs, on the 7th and 10th days for kidneys and at 10 days for liver. Data obtained showed that for the different tissues, improvement in the decrease of reduced glutathione (GSH) contents was obvious on the 10th day after irradiation. Treatment with Aloe vera was also effective in minimizing the radiation-induced increase in plasma glucose levels throughout the experimental period, while it has not ameliorated the increase in plasma insulin levels. It could be concluded that the synergistic relationship between the elements found in the leaf of Aloe vera could be a useful adjunct for maintaining the integrity of the antioxidant status.

  12. From the Cover: Aloin, a Component of the Aloe Vera Plant Leaf, Induces Pathological Changes and Modulates the Composition of Microbiota in the Large Intestines of F344/N Male Rats.

    Science.gov (United States)

    Boudreau, Mary D; Olson, Greg R; Tryndyak, Volodymyr P; Bryant, Matthew S; Felton, Robert P; Beland, Frederick A

    2017-08-01

    In a previous study, the oral administration of an Aloe vera whole leaf extract induced dose-related mucosal and goblet cell hyperplasia in the rat colon after 13 weeks and colon cancer after 2 years. The primary goal of this study was to determine whether or not the administration of aloin, a component of the Aloe vera plant leaf, would replicate the pathophysiological effects that were observed in rats in the previous study with an Aloe vera whole leaf extract. Groups of 10 male F344/N rats were administered aloin at 0, 6.95, 13.9, 27.8, 55.7, 111, 223, and 446 mg/kg drinking water for 13 weeks. At the end of study, rat feces were collected, and the composition of fecal bacteria was investigated by next generation sequencing of the PCR-amplified V3/V4 region of the 16S rRNA gene. At necropsy, blood was collected by cardiac puncture and organs and sections of the large intestine were collected for histopathology. Aloin induced dose-related increased incidences and severities of mucosal and goblet cell hyperplasia that extended from the cecum to the rectum, with increased incidences and severities detected at aloin doses ≥55.7 mg/kg drinking water. Analysis of the 16S rRNA metagenomics sequencing data revealed marked shifts in the structure of the gut microbiota in aloin-treated rats at each taxonomic rank. This study highlights the similarities in effects observed for aloin and the Aloe vera whole leaf extract, and points to a potential mechanism of action to explain the observed pathological changes via modulation of the gut microbiota composition. Published by Oxford University Press on behalf of the Society of Toxicology 2017. This work is written by US Government employees and is in the public domain in the US.

  13. Aloe-Emodin Induces Chondrogenic Differentiation of ATDC5 Cells via MAP Kinases and BMP-2 Signaling Pathways

    Science.gov (United States)

    Yang, Ming; Li, Liang; Heo, Seok-Mo; Soh, Yunjo

    2016-01-01

    Endochondral bone formation is the process by which mesenchymal cells condense into chondrocytes, which are ultimately responsible for new bone formation. The processes of chondrogenic differentiation and hypertrophy are critical for bone formation and are therefore highly regulated. The present study was designed to investigate the effect of aloe-emodin on chondrogenic differentiation in clonal mouse chondrogenic ATDC5 cells. Aloe-emodin treatment stimulated the accumulation of cartilage nodules in a dose-dependent manner. ATDC5 cells were treated with aloe-emodin and stained with alcian blue. Compared with the control cells, the ATDC5 cells showed more intense alcian blue staining. This finding suggested that aloe-emodin induced the synthesis of matrix proteoglycans and increased the activity of alkaline phosphatase. Aloe-emodin also enhanced the expressions of chondrogenic marker genes such as collagen II, collagen X, BSP and RunX2 in a time-dependent manner. Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK). Aloe-emodin also enhanced the protein expression of BMP-2 in a time-dependent manner. Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway. Aloe-emodin may have potential future applications for the treatment of growth disorders. PMID:27350340

  14. Structural and biochemical alterations during the healing process of tendons treated with Aloe vera.

    Science.gov (United States)

    Aro, A A; Nishan, U; Perez, M O; Rodrigues, R A; Foglio, M A; Carvalho, J E; Gomes, L; Vidal, B C; Pimentel, E R

    2012-10-29

    The tendon is composed of highly organized collagen fibers that form a complex supramolecular structure. After lesions, the organization and composition of the tendon are not completely restored. Our purpose was to evaluate if the application of Aloe vera improves tendon healing, considering the effectiveness in the stimulus of collagen synthesis. The calcaneal tendon of male Wistar rats was partially transected with subsequent topical application of A. vera ointment at the injury. The animals were separated into groups with tendons treated with the A. vera extract for 7days and excised on the 7th, 14th and 21st days after surgery; control rats received only ointment base without plant extract. Morphological analysis using polarization microscopy showed that the entire tendon undergoes a remodeling process, with disorganized collagen fibers by days 7 and 14 in plant-treated and non-treated groups and with a higher birefringence in tendons of the plant-treated group on the 21st day. A higher concentration of hydroxyproline was found in plant-treated tendons on days 7 and 14 compared with their controls. Western blots showed lower amounts of type I collagen in the plant-treated group on day 14 compared with the control. MMP-9 diminished 14days after lesion and the active isoform of MMP-2 increased on day 21 in plant-treated groups. The present study indicates a beneficial effect of A. vera in the tissue reorganization in the transected region of the tendon 21days after injury and is supported by an increase of active MMP-2. Copyright © 2012 Elsevier Inc. All rights reserved.

  15. Acute and sub-chronic toxicity studies of three plants used in Cameroonian ethnoveterinary medicine: Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) leaves, Carica papaya L. (Caricaceae) seeds or leaves, and Mimosa pudica L. (Fabaceae) leaves in Kabir chicks.

    Science.gov (United States)

    Nghonjuyi, Ndaleh Wozerou; Tiambo, Christian Keambou; Taïwe, Germain Sotoing; Toukala, Jean Paul; Lisita, Frederico; Juliano, Raquel Soares; Kimbi, Helen Kuokuo

    2016-02-03

    Aloe vera (L.) Burm. f. (Xanthorrhoeaceae), Carica papaya L. (Caricaceae) and Mimosa pudica L. (Fabaceae) are widely used in the Cameroonian ethnoveterinary medicine as a panacea, and specifically for gastrointestinal disorders as well as an anthelmintic and antibacterial. The present study evaluated the potential toxicity of the hydroalcoholic extracts of Aloe vera leaves, Carica papaya leaves or seeds, and Mimosa pudica leaves after acute and sub-chronic administration in chicks. For the acute toxicity test a single administration of each of the four hydroalcoholic extracts was given orally at doses ranging from 40 to 5120 mg/kg (n=5/group/sex). In the sub-chronic study, these extracts were given orally as a single administration to chicks at doses of 80, 160, 320 and 640 mg/kg/day for 42 days. The anti-angiogenic properties of these extracts (5-320 µg/mg) were investigated in the chick chorioallantoic membrane in vivo. In the acute toxicity test, none of the four studied hydroalcoholic extracts induced mortality or significant behavioural changes. The sub-acute treatment with the four plant extracts did not alter either the body weight gain or the food and water consumption. However, the results indicated that Aloe vera leaf extract acute treatment by oral route at doses up to 2560 mg/kg did not produce death in 50% (5/10) of chicks during 24h or 14 days of observation, but 20% (2/10) chicks died. The haematological and biochemical analyses did not show significant differences in any of the parameters examined in female or male groups, with the exception of a transient rise in white blood cell counts at high doses (640 mg/kg). Additionally, these extracts did not have the potential for anti-angiogenic effects through the inhibition of neo-angiogenesis in the chick chorioallantoic membrane in vivo. The results showed that the therapeutic use of the hydroalcoholic extracts of Aloe vera leaves, Carica papaya leaves or seeds and Mimosa pudica leaves had very low

  16. Comparison of aloe vera and omeprazole in the treatment of equine gastric ulcer syndrome.

    Science.gov (United States)

    Bush, J; van den Boom, R; Franklin, S

    2018-01-01

    Anecdotally, aloe vera is used to treat gastric ulceration, although no studies have yet investigated its efficacy in horses. To test the hypothesis that aloe vera would be noninferior to omeprazole in the treatment of equine gastric ulcer syndrome. Randomised, blinded clinical trial. Forty horses with grade ≥2 lesions of the squamous and/or glandular mucosa were randomly assigned to one of two groups. Horses received either aloe vera inner leaf gel (17.6 mg/kg bwt) b.i.d. or omeprazole (4 mg/kg bwt) s.i.d. for approximately 28 days, after which a repeat gastroscopic examination was performed to determine disease resolution. Horses with persistent lesions were offered a further 28 days of treatment with omeprazole (4 mg/kg bwt s.i.d.) and were re-examined on completion of treatment. Efficacy analyses were based on 39 horses that completed the trial. Equine squamous gastric disease (ESGD) was observed in 38 horses; improvement and healing rates in these horses were 56% and 17%, respectively, in the aloe vera group, and 85% and 75%, respectively, in the omeprazole group. Healing was less likely to occur in horses with prolonged gastric emptying. Equine glandular gastric disease (EGGD) was less common than ESGD (n = 14) and numbers were too small to perform meaningful statistical analyses. The hypothesis that aloe vera would be noninferior to omeprazole was not supported. No placebo control group was included. Limited numbers preclude any comment on the efficacy of aloe vera in the treatment of EGGD. Treatment with aloe vera was inferior to treatment with omeprazole. © 2017 EVJ Ltd.

  17. UP780, a chromone-enriched aloe composition improves insulin sensitivity.

    Science.gov (United States)

    Yimam, Mesfin; Zhao, Jifu; Corneliusen, Brandon; Pantier, Mandee; Brownell, Lidia Alfaro; Jia, Qi

    2013-08-01

    Diabetic individuals experience elevated fasting glucose, glycosylated hemoglobin (HbA1c), and plasma insulin and impaired glucose tolerance. Adiponectin is a hormone inversely correlated with insulin resistance. Here we describe the activity of aloesin, an aloe chromone that increases adiponectin production and, when formulated with an aloe polysaccharide composition, improves the insulin sensitivity in db/db and diet-induced obese-diabetic mice. Two aloe chromones, aloesin and aloesinol, were tested in vitro for adiponectin production. Following confirmation of glucose-lowering activity in a high-fat diet (HFD)-induced mouse model, aloesin was formulated with an Aloe vera inner leaf gel powder polysaccharide preparation to yield a composition designated UP780. Efficacy of UP780 was evaluated in HDF-induced and db/db mouse models. GW1929, a synthetic peroxisome proliferator-activated receptor-γ (PPARγ) agonist, was used as a positive control. After 3 weeks of treatment of HDF-induced mice, plasma insulin levels were decreased 37.9% and 46.7% by aloesin and aloesinol, respectively. In db/db mice, the chromone- (2% chromone:98% aloe polysaccharide) enriched UP780 aloe composition showed a 33.7% and 46.0% decrease in fasting triglyceride and plasma glucose levels after 10 weeks of oral treatment, respectively. Diabetic mice gavaged with 200 mg/kg of UP780 for 10 weeks showed a 30.3% decrease in fasting blood glucose levels and a 32.2% reduction in plasma insulin. In both animal models, UP780 showed a statistically significant improvement in blood glucose clearance. These findings indicate that UP780, a chromone-standardized, aloe-based composition, could potentially be used as a natural product option to facilitate the maintenance of healthy blood glucose levels.

  18. Effect of salicylic acid and aloe vera gel on postharvest quality of table grapes ( Vitis Vinifera

    Directory of Open Access Journals (Sweden)

    H. Peyro

    2017-06-01

    Full Text Available To investigate the effects of salicylic acid dipping and Aloe vera gel coating on shelf life and post harvest quality of table grapes (Vitis vinifera of the cultivar Shahroudi, a factorial experiment was conducted on the basis of randomized complete blocks design with three factors and three replicates in agricultural faculty of Islamic Azad University in 2014. The treatments were dipping in Salicylic acid (three levels of 0, 1 and 2 mmmol-1 for 15 minutes and coating with Aloe vera gel (four levels of 0, 10%, 15% and 20% w/v and measurement of traits in 1st day, 30th day and 60th day after treatment of berries. The results showed that the interaction effect of salicylic acid and Aloe vera gel application was significant on all of traits except for pH value in a way that the best and the minimum weight loss (0.09g was obtained by application of 2 mmol-1 Salicylic acid and 20% Aloe vera gel in 1st day after treatment. The greatest amount of total soluble solids (428.43 g.100g-1 fruit juice was found in 2 mmol-1 Salicylic acid and 15% Aloe vera gel in 60th day. The highest Catalase enzyme activity (0.0013 Ua.mg-1Pro was attained in 2 mmol-1 Salicylic acid and 15% Aloe vera gel in 30th day. These results demonstrated that treatment of grape berries by salicylic acid and Aloe vera gel had positive effect on shelf life of table grapes and their postharvest quality

  19. Therapeutic effects of Aloe spp. in traditional and modern medicine: A review.

    Science.gov (United States)

    Akaberi, Maryam; Sobhani, Zahra; Javadi, Behjat; Sahebkar, Amirhossein; Emami, Seyed Ahmad

    2016-12-01

    Traditional medicine is a useful guide in medical sciences. In the Islamic Iranian traditional medicine, the medicinal properties of many plants have been mentioned that could be exploited in drug discovery. We aimed to explore the nature and properties of Aloe spp. As described in some major Islamic traditional texts including Ferdows al-Hekmah fi'l-Tibbe (The Paradise of Wisdom in Medicine), Al-Hawi fi'l-Tibb (Comprehensive Book of Medicine), Kamel al-Sanaat al-Tibbyyah (Complete Book of the Medical Art), Al-Qanun fi'l-Tibb (Canon of Medicine), Zakhireh Kharazmshahi (Treasure of Kharazmshah), and Makhzan al-Adwiah (Drug Treasure), and assess the conformity of traditional medicine instructions with the findings of modern pharmacological studies. Gastrointestinal activities, hepato-protective properties, beneficial effects against skin problems such as wounds, injuries, and infective diseases are among the most frequently mentioned properties of Aloe spp. Several activities of Aloe spp. described in traditional medicine have been the subject of recent in vitro and in vivo studies as well as clinical trials. Owing to the positive findings, different preparations of Aloe spp. are now present in pharmaceutical markets such as Aloe cosmetic products. On the other hand, there are many traditional therapeutic effects of Aloe spp. which have not been studied and require confirmatory experimental or clinical investigations. It is hoped that the present study could stimulate further research on the unexplored aspects of the medicinal properties of Aloe spp. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  20. Involvement of NF-κB and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, α-mangostin, from Cratoxylum arborescens [Retraction

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    Ibrahim MY

    2017-05-01

    Full Text Available Ibrahim MY, Hashim NM, Mohan S, et al. Involvement of NF-κB and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, α-mangostin, from Cratoxylum arborescens. Drug Design, Development and Therapy. 2014;8:2193–2211 was published subsequent to Ibrahim MY, Hashim NM, Mohan S, et al. α-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-κB and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Design, Development and Therapy. 2014;8:1629–1647.When comparing the papers it becomes apparent that they have an unacceptably high degree of similarity and re-use. Further, there is no clear scientific distinction between the cell lines and the results in both. Accordingly, the Editor-in-Chief and Publisher have issued this Notice of Retraction.This retraction relates to this paperThis retraction relates to this Corrigendum