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Sample records for external egg quality

  1. Phenotypic correlations between egg weight and some egg quality ...

    African Journals Online (AJOL)

    Eggs were examined for both internal and external egg quality traits.Data obtained were subjected to one-way analysis of variance using the general linear procedure of SAS (2012). Differences in means were ranked using the Duncan's multiple Range test. Phenotypic correlations between egg weight and other egg quality ...

  2. Egg shell quality in Japanese quail: characteristics, heritabilities and genetic and phenotypic relationships.

    Science.gov (United States)

    Narinc, D; Aygun, A; Karaman, E; Aksoy, T

    2015-07-01

    The objective of the present study was to estimate heritabilities as well as genetic and phenotypic correlations for egg weight, specific gravity, shape index, shell ratio, egg shell strength, egg length, egg width and shell weight in Japanese quail eggs. External egg quality traits were measured on 5864 eggs of 934 female quails from a dam line selected for two generations. Within the Bayesian framework, using Gibbs Sampling algorithm, a multivariate animal model was applied to estimate heritabilities and genetic correlations for external egg quality traits. The heritability estimates for external egg quality traits were moderate to high and ranged from 0.29 to 0.81. The heritability estimates for egg and shell weight of 0.81 and 0.76 were fairly high. The genetic and phenotypic correlations between egg shell strength with specific gravity, shell ratio and shell weight ranging from 0.55 to 0.79 were relatively high. It can be concluded that it is possible to determine egg shell quality using the egg specific gravity values utilizing its high heritability and fairly high positive correlation with most of the egg shell quality traits. As a result, egg specific gravity may be the choice of selection criterion rather than other external egg traits for genetic improvement of egg shell quality in Japanese quails.

  3. Effect of commercial diets on egg quality characteristics | Ayanwale ...

    African Journals Online (AJOL)

    The effect of four commercial feeds in Niger State Nigeria on egg quality characteristics was studied. The feed designated diets 1, 2, 3 and 4 were fed to Rhode Island Red (RIR) hens and their eggs were collected as from 38 weeks of age for 4 weeks. A total of 384 eggs were analysed for both external and internal egg ...

  4. Effect of genotype on egg quality characteristics of guinea fowl ...

    African Journals Online (AJOL)

    The genotypes were Pearl x Pearl (PL x PL), Black x Black (BL x BL) and Pearl x Black (PL x BL). ... The external and shell quality parameters measured were egg weight (EWGT), egg length (ELGT), egg width (EWDT), egg shape index (ESPI), shell weight (SWGT), and shell thickness (STKN) while the internal traits were ...

  5. QUALITY ASSESSMENT OF EGGS PACKED UNDER MODIFIED ATMOSPHERE

    Directory of Open Access Journals (Sweden)

    Aline Giampietro-Ganeco

    2015-02-01

    Full Text Available Eggs are perishable foods and lose quality quickly if not stored properly. From the moment of posture to the marketing of egg, quality loss occurs through gas exchange and water through the pores of the shell with the external environment and thus, studies involving modified atmosphere packaging are extremely important. The aim of the present study is to assess the internal quality of eggs packed under modified atmosphere and stored at room temperature. Six hundred and twelve fresh commercial eggs from 38-week old Hisex White laying hens were used. The present study was conducted in a completely randomized experimental design in a 4 x 4 + 1 factorial arrangement [vacuum, vacuum with oxygen gas (O2 absorbent, vacuum with oxygen gas (O2 and carbon dioxide (CO2 absorbents, vacuum with oxygen gas (O2 absorbent and carbon dioxide (CO2 gas generator; storage times (7, 14, 21 and 28 days, control group (fresh eggs], with three repetitions (12 eggs each. The following factors were assessed: weight maintenance during storage; Haugh unit; color and yolk index. The modified atmosphere packaging maintained the weight of the eggs during a period of 28 days. Vacuum packaging with O2 absorbent and CO2 gas generator was more efficient in maintenance of egg quality, based on the values ​​of Haugh unit and yolk index. The luminosity of the yolk was preserved during the 28 storage days in all of the packaging types used. The vacuum packaging with O2 gas absorbent and CO2 gas generator provide an increase in internal egg quality.

  6. Egg production and egg quality in free-range laying hens housed at different outdoor stocking densities.

    Science.gov (United States)

    Campbell, D L M; Lee, C; Hinch, G N; Roberts, J R

    2017-09-01

    Free-range laying hen systems are increasing in number within Australia. Variation in outdoor stocking densities has led to development of a national information standard on free-range egg labeling, including setting a maximum density of 10,000 hens per hectare. However, there are few data on the impacts of differing outdoor densities on production and egg quality. ISA Brown hens in small (150 hens) flocks were housed in identical indoor pens, each with access (from 21 weeks) to different sized ranges simulating one of three outdoor stocking densities (2 replicates each: 2,000 hens/hectare (ha), 10,000 hens/ha, 20,000 hens/ha). Hen-day production was tracked from 21 through 35 weeks with eggs visually graded daily for external deformities. All eggs laid on one day were weighed each week. Eggs were collected from each pen at 25, 30, and 36 weeks and analyzed for egg quality. There were no effects of outdoor stocking density on average hen-day percentage production (P = 0.67), egg weight (P = 0.09), percentages of deformed eggs (P = 0.30), shell reflectivity (P = 0.74), shell breaking strength (P = 0.07), shell deformation (P = 0.83), or shell thickness (P = 0.24). Eggs from hens in the highest density had the highest percentage shell weight (P = 0.004) and eggs from the lowest density had the highest yolk color score (P egg quality is warranted. © 2017 Poultry Science Association Inc.

  7. Layer performance, fatty acid profile and the quality of eggs from ...

    African Journals Online (AJOL)

    It was concluded that, although MOWSM inclusion improved yolk colour, maintained external egg quality, and improved the fatty acid profile, the deleterious effect that it had on layer performance indicated that it may not be fed to early-lay hens at these respective levels. Keywords: Alternative protein, egg production, laying ...

  8. Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio.

    Directory of Open Access Journals (Sweden)

    Ozlem Yilmaz

    Full Text Available Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated sample sets whose protein extracts were subjected to different levels of fractionation before liquid chromatography and tandem mass spectrometry. Obtained spectra were searched against a zebrafish proteome database and detected proteins were annotated, categorized and quantified based on normalized spectral counts. Manually curated and automated enrichment analyses revealed poor quality eggs to be deficient of proteins involved in protein synthesis and energy and lipid metabolism, and of some vitellogenin products and lectins, and to have a surfeit of proteins involved in endo-lysosomal activities, autophagy, and apoptosis, and of some oncogene products, lectins and egg envelope proteins. Results of pathway and network analyses suggest that this aberrant proteomic profile results from failure of oocytes giving rise to poor quality eggs to properly transit through final maturation, and implicated Wnt signaling in the etiology of this defect. Quantitative comparisons of abundant proteins in good versus poor quality eggs revealed 17 candidate egg quality markers. Thus, the zebrafish egg proteome is clearly linked to embryo developmental potential, a phenomenon that begs further investigation to elucidate the root causes of poor egg quality, presently a serious and intractable problem in livestock and human reproductive medicine.

  9. Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio).

    Science.gov (United States)

    Yilmaz, Ozlem; Patinote, Amélie; Nguyen, Thao Vi; Com, Emmanuelle; Lavigne, Regis; Pineau, Charles; Sullivan, Craig V; Bobe, Julien

    2017-01-01

    Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated sample sets whose protein extracts were subjected to different levels of fractionation before liquid chromatography and tandem mass spectrometry. Obtained spectra were searched against a zebrafish proteome database and detected proteins were annotated, categorized and quantified based on normalized spectral counts. Manually curated and automated enrichment analyses revealed poor quality eggs to be deficient of proteins involved in protein synthesis and energy and lipid metabolism, and of some vitellogenin products and lectins, and to have a surfeit of proteins involved in endo-lysosomal activities, autophagy, and apoptosis, and of some oncogene products, lectins and egg envelope proteins. Results of pathway and network analyses suggest that this aberrant proteomic profile results from failure of oocytes giving rise to poor quality eggs to properly transit through final maturation, and implicated Wnt signaling in the etiology of this defect. Quantitative comparisons of abundant proteins in good versus poor quality eggs revealed 17 candidate egg quality markers. Thus, the zebrafish egg proteome is clearly linked to embryo developmental potential, a phenomenon that begs further investigation to elucidate the root causes of poor egg quality, presently a serious and intractable problem in livestock and human reproductive medicine.

  10. A general solution for optimal egg size during external fertilization, extended scope for intermediate optimal egg size and the introduction of Don Ottavio 'tango'

    NARCIS (Netherlands)

    Luttikhuizen, PC; Honkoop, PJC; Drent, J; van der Meer, J

    2004-01-01

    Egg sizes of marine invertebrates vary greatly, both within and between species. Among the proposed causes of this are a trade-off between egg size, egg number and survival probability of offspring, and a selection pressure exerted by sperm limitation during external fertilization. Although larger

  11. Effect of organic selenium and zinc on the performance and egg quality of Japanese quails

    Directory of Open Access Journals (Sweden)

    VC Cruz

    2011-06-01

    Full Text Available The present study aimed at evaluating the effect of the dietary inclusion of organic trace minerals selenium and zinc on the performance and internal and external egg quality of Japanese quails submitted to heat stress. Data on egg production, feed conversion (kg feed intake/kg eggs and dozen eggs, egg weight, egg specific gravity, eggshell thickness and weight, Haugh unit, yolk index, albumen index and mortality (% of 144 quails were evaluated for 112 days, divided in eight cycles of 14 days. Birds were distributed according to a randomized block experimental design into four treatments (control; 0.3ppm Se; 60ppm Zn and 0.3ppm Se + 60ppm Zn with six replicates each. There were no differences (p> 0.05 in egg production (%, egg mass (g/hen/day, feed conversion per egg mass (kg/kg, feed conversion per dozen eggs (kg/dz, average egg weight (g, egg specific gravity, eggshell thickness and weight (g, Haugh unit, yolk index, albumen index and mortality (%. However, quails fed the combination of Se and Zn presented higher (p < 0.05 feed intake (28.73 g/hen/day. Those fed only organic selenium had higher average daily egg production (30.17 eggs/day, and those fed the diet only supplemented with zinc presented higher mortality (p < 0.05. The results of the present study suggest that the supplementation of organic trace minerals in Japanese quails diets submitted to heat stress does not significantly influence quail performance and internal egg quality, whereas the supplementation of the combination of organic Zn and Se increases feed intake.

  12. Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio)

    OpenAIRE

    Yilmaz, Ozlem; Patinote, Amélie; Nguyen, Thao Vi; Com, Emmanuelle; Lavigne, Regis; Pineau, Charles; Sullivan, Craig V.; Bobe, Julien

    2017-01-01

    Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated sample sets whose protein extracts were subjected to different levels of fractionation before liquid chr...

  13. Haugh Unit: Gold Standard of Egg Quality

    Science.gov (United States)

    Rapidly determining shell egg quality in an objective manner is not an easy task. Candling is most often utilized as a quick method for assessing egg quality in a non-destructive manner, but it is a highly subjective method. As you have experienced this week, when candling, it is almost impossibl...

  14. Quality traits of eggs from autosexing Easter eggers

    Directory of Open Access Journals (Sweden)

    H. Lukanov

    2016-09-01

    Full Text Available Abstract. The main egg quality traits were investigated in two autosexing F1 Easter egger crosses. Partridge Araucana roosters were used as carriers of the eggshell biliverdin pigmentation gene. Maternal forms used for obtaining the two experimental crosses were high-producing layer hens carrying the sex-linked S and B genes. Experimental groups comprised F1 crosses of partridge Araucana roosters with white mutational Rhode Island hens or hens with barred colour mutation. The aim of the study was to investigated the quality traits of blue-green eggs produced by autosexing hens. The egg quality was evaluated at 38 weeks of age. Studied traits comprised egg weight (g, shape index, albumen index (AI, Haugh units (HU, yolk index (YI, yolk colour (Roche scale, 2 2 percentages of albumen, yolk and eggshell (%, eggshell surface (cm , eggshell density (mg/cm , average eggshell thickness (µm and eggshell colour. The group W was outlined with the highest egg weight – 61.03±0.47 g, and group A – with the lowest (50.91±0.32 g. The albumen and yolk quality was the best in group A, where albumen index was 0.075±0.002, Haugh units – 76.53±0.8 and yolk index – 0.491±0.009. The analysis of eggshell colour showed that eggs of group A were with the highest lightness (L* value (73.60±0.77, whereas eggs of groups W and AW had egg lightness of 60.45±0.53 and 61.11±0.55 (p<0.001 respectively. The shell colour index (SCI demonstrated a certain overlap of values in eggs with protoporphyrin and biliverdin taints. After introduction of a correction coefficient and recalculation of SCI*, the values of green eggs assumed a negative sign and could be distinguished from brown eggs.

  15. Body weight, egg production, and egg quality traits of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica in coastal climatic condition of Odisha

    Directory of Open Access Journals (Sweden)

    Jessy Bagh

    2016-08-01

    Full Text Available Aim: The present study was conducted to evaluate the performance of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica with respect to body weight, egg production, and egg quality traits in the coastal climatic condition of Odisha. Materials and Methods: A total of 500-day-old straight run Japanese quail chicks of three varieties, viz., gray, brown, and white were randomly selected and reared in deep litter system at Central Poultry Development Organization, Eastern Region, Bhubaneswar. The weekly body weight of the birds was recorded till their egg production stage (up to 6 weeks of age. The average egg production was recorded every biweekly from 6th to 20th week. Exterior and interior quality of eggs from each variety was determined at 6 weeks of age. Results: The initial average weekly body weight of three varieties did not differ (p>0.05 among the varieties. However, from 1st to 6th week significantly higher body weight was observed in gray than white and brown. Brown varieties had reached 50% egg production 1 week earlier than gray and white. Brown had higher peak hen day (HD production or henhoused egg production followed by white and gray. External quality such as: Egg weight, egg length, egg width, volume, shape index, shell weight, shell thickness depicted no significant difference among the varieties except circumference length and circumference width, which were significantly higher (p≤0.05 in gray varieties than brown varieties. Internal egg characteristics such as: Albumen length, albumen width, albumen height, albumen index, yolk length, yolk width, yolk height, yolk index, albumen weight, yolk weight, Haugh unit revealed no significance difference among the varieties. Conclusion: It may be summarized from the findings that gray excelled in body weight followed by white and brown. Egg production potential in terms of hen house egg production or HD egg production was higher for brown followed by white

  16. EGG QUALITY OF CREOLE HENS REARED IN THE BACKYARD

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    Aureliano Juárez-Caratachea

    2009-09-01

    Full Text Available The egg quality from Creole backyard hens was determined by collecting samples from 11 municipalities from the central area of Michoacan State, Mexico. It was measured: egg weight (EW, longitude diameter (LD of the egg, transversal diameter (TD of the egg, shell weight (SW, shell thickness (ST, shell index (SI, white diameter (WD, yolk diameter (YD, white high (WH, yolk high (YH, air chamber high (ACH and Haugh units (HU. The averages obtained were: EW 50.7 g; LD 5.6 cm; TD 4.2 cm; SW 4.5 g.; ST 0.28 mm; SI 8.9 %; WD 10.0 cm; YD 4.5 cm; WH 6.3 mm; YH 1.6 cm; ACH 5.4 mm; HU 73.4, YP 12.1. In conclusion, the eggs of backyard hens were smaller than commercial eggs. However, the internal quality indicators suggested that backyard hen eggs have similar quality to that of commercial hens.

  17. Effect of Olive Leaf ( Powder on Laying Hens Performance, Egg Quality and Egg Yolk Cholesterol Levels

    Directory of Open Access Journals (Sweden)

    H. Cayan

    2015-04-01

    Full Text Available This experiment was conducted to measure the effects of olive leaf powder on performance, egg yield, egg quality and yolk cholesterol level of laying hens. A total of 120 Lohmann Brown laying hens of 22 weeks old were used in this experiment. The birds were fed on standard layer diets containing 0, 1%, 2%, or 3% olive leaf powder for 8 weeks. Egg weight and yield were recorded daily; feed intake weekly; egg quality and cholesterol content at the end of the trial. Olive leaf powder had no effect on feed intake, egg weight, egg yield and feed conversion ratio (p>0.05 while olive leaf powder increased final body weight of hens (p0.05. To conclude, olive leaf powder can be used for reducing egg yolk cholesterol content and egg yolk coloring agent in layer diets.

  18. PHENOTYPIC CORRELATIONS BETWEEN EGG WEIGHT AND ...

    African Journals Online (AJOL)

    IBUKUN

    variety) obtained from 60 eight weeks old Japanese quails of three different plumage colour varieties: Pharaoh Quail (Black), A & M white (White) and Manchurian Gold (Brown) at 8-14 weeks of age were used in this study. Eggs were examined for both internal and external egg quality traits.Data obtained were subjected to ...

  19. Quality of foraging material and the effect on hens feed intake, egg production and - quality

    DEFF Research Database (Denmark)

    Steenfeldt, Sanna; Hammershøj, Marianne

    2010-01-01

    In a project with organic egg laying hens, the effect of different kind of foraging material was studied on feed intake, egg-production and -quality. Udgivelsesdato: August......In a project with organic egg laying hens, the effect of different kind of foraging material was studied on feed intake, egg-production and -quality. Udgivelsesdato: August...

  20. Methods for external disinfection of blow fly (Diptera: Calliphoridae) eggs prior to use in wound debridement therapy.

    Science.gov (United States)

    Brundage, Adrienne L; Crippen, Tawni L; Tomberlin, Jeffery K

    2016-03-01

    Maggot debridement therapy (MDT) is the use of the larval stage of flies (i.e., Calliphoridae) to remove necrotic tissue and disinfect wounds. Effective MDT requires aseptic technique to prevent the unintentional introduction of pathogenic bacteria into a wound to be debrided; yet the external surface of Calliphoridae eggs is often heavily contaminated with bacteria. Studies of external disinfection of dipteran eggs have been reported, but neither their efficacy nor effect on egg viability has been adequately assessed. The present study evaluated the efficacy of ten disinfection techniques involving immersion, rinse, or a combination of both in formalin, Lysol, formaldehyde, bleach, ethanol, Sporgon, or benzalkonium chloride. All techniques resulted in significant decreases in culturable, aerobic bacterial load on Lucilia cuprina eggs. For L. cuprina, a 10 minute 3% Lysol immersion was the most efficacious, disinfecting 96.67% of egg samples, while resulting in 31.84% egg mortality. The 5% formalin immersion was least efficacious, disinfecting only 3.33% of L. cuprina egg samples, while resulting in 33.51% egg mortality. A formaldehyde immersion, one of the most commonly used disinfection techniques, was moderately effective, disinfecting 66.7% of egg samples, while resulting in 40.16% egg mortality. For Chrysomya rufifacies and Cochliomyia macellaria egg samples, the 10 minute 3% Lysol immersion disinfected 100% of the samples, and for Lucilia sericata, 80% of egg samples, while resulting in 33.97%, 7.34%, and 36.96% egg mortality, respectively. H2 CO disinfected 16.67% of Ch. rufifacies, 26.67% of C. macellaria, and 56.67% of L. sericata egg samples, while resulting in 21.98%, 10.18%, and 32.19% egg mortality, respectively. Due to its high disinfection efficacy and relatively low egg mortality, a 10 minutes 3% Lysol immersion is recommended for sterilizing Calliphoridae eggs prior to rearing of larvae for use in MDT. © 2016 by the Wound Healing Society.

  1. Impacts of Limestone Multi-particle Size on Production Performance, Egg Shell Quality, and Egg Quality in Laying Hens

    Directory of Open Access Journals (Sweden)

    X. Y. Guo

    2012-06-01

    Full Text Available This experiment was conducted to evaluate the effects of single or multi-particle size limestone on the egg shell quality, egg production, egg quality and feed intake in laying hens. A total of 280 laying hens (ISA brown were used in this 10-wk trial. Laying hens were randomly assigned to 4 treatments with 14 replications per treatment and 5 adjacent cages as a replication (hens were caged individually. The experimental treatments were: i L, basal diet+10% large particle limestone; ii LS1, basal diet+8% large particle limestone+2% small particle limestone; iii LS2, basal diet+6% large particle limestone+4% small particle limestone; iv S, basal diet+10% small particle limestone. The egg production was unaffected by dietary treatments. The egg weight in S treatment was lighter than other treatments (p<0.05. The egg specific gravity in S treatment was lower than other treatments (p<0.05. The eggshell strength and eggshell thickness in S treatment were decreased when compared with other dietary treatments (p<0.05. The laying hens in LS1 and LS2 treatment had a higher average feed intake than the other two treatments (p<0.05. Collectively, the dietary multi-particle size limestone supplementation could be as efficient as large particle size limestone.

  2. Effect of a commercial housing system on egg quality during extended storage.

    Science.gov (United States)

    Jones, D R; Karcher, D M; Abdo, Z

    2014-05-01

    Egg producers in the United States are utilizing a variety of commercial egg production systems to provide consumer choice and meet legislative requirements. Consumer egg grades in the United States were developed for conventional cage production, and it is unclear what effect alternative production systems might have on egg quality during retail and consumer home storage. The current study was undertaken to determine what changes in egg quality characteristics occur during extended cold storage for commercially produced conventional cage, enriched colony cage, and cage-free aviary eggs. During 12 wk of cold storage, egg weight, albumen height, Haugh unit, static compression shell strength, vitelline membrane strength and deformation, yolk index, shell dynamic stiffness, and whole egg total solids were monitored. Overall, aviary and enriched eggs were significantly (P system effects for yolk measurements, shell dynamic stiffness, or whole egg total solids were observed. Albumen height, Haugh unit, and yolk quality measurements were all greatest at 0 and lowest at 12 wk of storage (P systems for each measured attribute at 4, 6, and 12 wk was determined. Other than differences in the change of egg weight at 4 wk, no significant differences in the rate of quality decline were found among the housing systems. The results of the current study indicate that current US egg quality standards should effectively define quality for commercially produced conventional cage, enriched colony cage, and cage-free aviary eggs.

  3. Determining storage related egg quality changes via digital image ...

    African Journals Online (AJOL)

    Area and length measurements related to exterior and interior egg quality were determined by digital image analysis. In general, excluding the outer thin albumen area, all of the area measurements such as total egg content area and inner thick albumen area were larger in stored eggs than in fresh eggs (52.28 vs.

  4. Express quality control of chicken eggs by machine vision

    Science.gov (United States)

    Gorbunova, Elena V.; Chertov, Aleksandr N.; Peretyagin, Vladimir S.; Korotaev, Valery V.; Arbuzova, Evgeniia A.

    2017-06-01

    The urgency of the task of analyzing the foodstuffs quality is determined by the strategy for the formation of a healthy lifestyle and the rational nutrition of the world population. This applies to products, such as chicken eggs. In particular, it is necessary to control the chicken eggs quality at the farm production prior to incubation in order to eliminate the possible hereditary diseases, as well as high embryonic mortality and a sharp decrease in the quality of the bred young. Up to this day, in the market there are no objective instruments of contactless express quality control as analytical equipment that allow the high-precision quality examination of the chicken eggs, which is determined by the color parameters of the eggshell (color uniformity) and yolk of eggs, and by the presence in the eggshell of various defects (cracks, growths, wrinkles, dirty). All mentioned features are usually evaluated only visually (subjectively) with the help of normalized color standards and ovoscopes. Therefore, this work is devoted to the investigation of the application opportunities of contactless express control method with the help of technical vision to implement the chicken eggs' quality analysis. As a result of the studies, a prototype with the appropriate software was proposed. Experimental studies of this equipment on a representative sample of eggs from chickens of different breeds have been carried out (the total number of analyzed samples exceeds 300 pieces). The correctness of the color analysis was verified by spectrophotometric studies of the surface of the eggshell.

  5. Effect of egg washing on the cuticle quality of brown and white table eggs.

    Science.gov (United States)

    Leleu, S; Messens, W; De Reu, K; De Preter, S; Herman, L; Heyndrickx, M; De Baerdemaeker, J; Michiels, C W; Bain, M

    2011-10-01

    Egg washing is currently not permitted within the European Union, with few exceptions. This is mainly because there are concerns that cuticle damage could occur during or after the washing process, as a result of a suboptimal operation. In this study, the cuticle coverage levels of 400 washed or unwashed eggs, derived from either a brown or a white egg-laying flock at the end of lay, were compared. The eggs from older hens inherently have poorer cuticle coverage and as a result arguably constitute a greater risk to consumer safety if they are then washed. Thus, the effects of the washing procedure used in this study on cuticle quality were tested under the worst-case scenario. A standard Swedish egg washing process was used. The cuticle coverage of the eggs was assessed by a colorimeter by quantifying the color difference before and after staining with Tartrazine and Green S. The cuticle of an additional 30 eggs from each of the four groups was then visually assessed by scanning electron microscopy. The staining characteristics of the cuticle varied greatly within each group of eggs and showed that the washing process did not lead to cuticle damage. Scanning electron microscopy confirmed that there was no irreversible damage to the cuticle of the washed eggs and that it was not possible to correctly assign the treatment (washed or not) based on a visual assessment. In conclusion, no evidence could be found to suggest that the washing procedure used in this investigation irreversibly changed the quality of the cuticle.

  6. Quality Properties of Cakes Containing Gamma-Irradiated Egg White

    International Nuclear Information System (INIS)

    Lee, J.W.; Seo, J.H.; Ahn, H.J; Byun, M.W; Kim, Y.H.; Choi, J.M.; Yook, H.S.

    2003-01-01

    As a research on the practical approaches of gamma irradiation for the reduction of egg allergy, cakes including gamma-irradiated egg white were manufactured, and rheological characteristics and sensory qualities of the cakes were evaluated. Egg white was separated from whole egg and then gamma-irradiated with the absorbed dose of 10 or 20 kGy

  7. Egg size and offspring quality: a meta-analysis in birds.

    Science.gov (United States)

    Krist, Miloš

    2011-08-01

    Parents affect offspring fitness by propagule size and quality, selection of oviposition site, quality of incubation, feeding of dependent young, and their defence against predators and parasites. Despite many case studies on each of these topics, this knowledge has not been rigorously integrated into individual parental care traits for any taxon. Consequently, we lack a comprehensive, quantitative assessment of how parental care modifies offspring phenotypes. This meta-analysis of 283 studies with 1805 correlations between egg size and offspring quality in birds is intended to fill this gap. The large sample size enabled testing of how the magnitude of the relationship between egg size and offspring quality depends on a number of variables. Egg size was positively related to nearly all studied offspring traits across all stages of the offspring life cycle. Not surprisingly, the relationship was strongest at hatching but persisted until the post-fledging stage. Morphological traits were the most closely related to egg size but significant relationships were also found with hatching success, chick survival, and growth rate. Non-significant effect sizes were found for egg fertility, chick immunity, behaviour, and life-history or sexual traits. Effect size did not depend on whether chicks were raised by their natural parents or were cross-fostered to other territories. Effect size did not depend on species-specific traits such as developmental mode, clutch size, and relative size of the egg, but was larger if tested in captive compared to wild populations and between rather than within broods. In sum, published studies support the view that egg size affects juvenile survival. There are very few studies that tested the relationship between egg size and the fecundity component of offspring fitness, and no studies on offspring survival as adults or on global fitness. More data are also needed for the relationships between egg size and offspring behavioural and

  8. Microbial quality and prevalence of Salmonella and Listeria in eggs

    Directory of Open Access Journals (Sweden)

    Manijeh Mahdavi

    2012-01-01

    Full Text Available Aims: This study was undertaken to determine the microbial quality and the prevalence of Salmonella and Listeria in table eggs in Isfahan, Iran. Materials and Methods: A total of 525 samples were randomly collected from various shops in Isfahan, Iran. Microbial quality of eggs evaluated by coliform count and total bacterial viable counts. Also, detection of Listeria and Salmonella in egg contents and on eggs shells was performed. Results: The mean of total viable bacteria and coliform counts in the egg contents were 3.95 × 10 4 CFU/g and 4.94 × 10 3 CFU/g, respectively. Salmonella and Listeria were not found on the shell or content of eggs. Enterobacteriaceae families were found in 357 of 525 (68.28% and 276 of 525 (52.44% of egg shell and egg content samples, respectively. Moreover, Pseudomonas aeruginosa was isolated from 175 (33.41% and 144 (25.37% of egg shell and egg content, respectively. The isolated Enterobacteriaceae were included: Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Buttiauxella agrestis, Cedecea lapagei, Cedecea davisae and Erwinia herbicola. Conclusion: The findings of the present study indicate although Salmonella and Listeria were not found in egg samples; however, there is an urgent need to improve the hygienic level of consumed eggs.

  9. Quality of spelt pasta enriched with eggs and identification of eggs using 13C MAS NMR spectroscopy

    Directory of Open Access Journals (Sweden)

    Filipović Jelena S.

    2015-01-01

    Full Text Available This paper deals with the characteristics of spelt pasta enriched with eggs. Eggs were added to spelt farina in the quantity of 0, 124 or 248 g/kg (equivalent to 0, 3 or 6 eggs, respectively. Post-hoc Tukey’s HSD test at 95% confidence limit showed significant differences between various samples. Relatively low coefficients of variation have been obtained for each applied assay (1.25-12.42%, which confirmed the high accuracy measurements and statistically significant results. Standard score analysis is applied for accessing the contribution of eggs content to spelt pasta quality. Maximum scores regarding quality (0.89 and chemical characteristics (0.70, have been obtained for 6 eggs spelt pasta formulation. It is also shown that the presence of eggs in pasta can be clearly confirmed by 13C MAS NMR spectroscopy. Simultaneous increase in area of peak positioned at 29.5 and 176 ppm is directly associated with the increase in the content of added eggs in the corresponding samples. Pertinent data point at positive contribution of eggs to the spelt pasta and also that NMR spectrum can be used in the egg quantity control. [Projekat Ministarstva nauke Republike Srbije, br. TRI 46005 i br. TR 31029

  10. Gamma radiation influence on internal quality factors of eggs

    International Nuclear Information System (INIS)

    Pombo, Carolina R.; Mano, Sergio B.

    2005-01-01

    Poultry egg is a fundamental item in the human diet due to its high nutritional value. Its treatment by irradiation in order to eliminate pathogenic bacteria has been authorized in the USA in 2000 limited to a dose of 3 kGy in contrast to the requirements of the Brazilian legislation that does not establish quantitative dose limits. However research on the sensory properties of irradiated eggs is still scarce in the literature. In this work, possible changes in the internal quality factors of eggs were investigated. The samples were irradiated with doses of 0, 0.5, 1.0, 1.5, 2.0 and 2.5 kGy at an average dose rate of 1.7 kGy/h in the research Cesium-137 irradiation facility at the Technological Center of the Army (CTEx) situated in Guaratiba, Rio de Janeiro (RJ). A total of 300 eggs sorted in 6 lots, one for each dose, were kept at room temperature being periodically inspected up to 20 days. The samples were analyzed for the following indicators of the internal quality of the eggs: Haugh unity, air chamber, yolk index, pH of the white and pH of the yolk. The results indicated that irradiation damages the structure of the white of the eggs reducing its consistency in addition to changing other parameters related to their internal quality. Therefore, it has been concluded that irradiation is not appropriate for the treatment of whole eggs in the conditions studied in this work. (author)

  11. Quality characteristic of spray-drying egg white powders.

    Science.gov (United States)

    Ma, Shuang; Zhao, Songning; Zhang, Yan; Yu, Yiding; Liu, Jingbo; Xu, Menglei

    2013-10-01

    Spray drying is a useful method for developing egg process and utilization. The objective of this study was to evaluate effects on spray drying condition of egg white. The optimized conditions were spraying flow 22 mL/min, feeding temperature 39.8 °C and inlet-air temperature 178.2 °C. Results of sulfydryl (SH) groups measurement indicated conformation structure have changed resulting in protein molecule occur S-S crosslinking phenomenon when heating. It led to free SH content decreased during spray drying process. There was almost no change of differential scanning calorimetry between fresh egg white and spray-drying egg white powder (EWP). For a given protein, the apparent SH reactivity is in turn influenced by the physico-chemical characteristics of the reactant. The phenomenon illustrated the thermal denaturation of these proteins was unrelated to their free SH contents. Color measurement was used to study browning level. EWP in optimized conditions revealed insignificant brown stain. Swelling capacity and scanning electron micrograph both proved well quality characteristic of spray-drying EWP. Results suggested spray drying under the optimized conditions present suitable and alternative method for egg processing industrial implementation. Egg food industrialization needs new drying method to extend shelf-life. The purpose of the study was to provide optimal process of healthy and nutritional instant spray-drying EWP and study quality characteristic of spray-drying EWP.

  12. Storage of Hatching Eggs : Effects of storage and early incubation conditions on egg characteristics, embryonic development, hatchability, and chicken quality

    NARCIS (Netherlands)

    Reijrink, I.A.M.

    2010-01-01

    Key words: egg storage, embryonic development, albumen quality, hatchability, chick quality

    It is well known that an increase in the storage duration increases incubation duration and decreases hatchability and chick quality. The negative effects of prolonged egg storage (> 7 days)

  13. Application of probiotic on egg production and egg quality of chukar ...

    African Journals Online (AJOL)

    ajl yemi

    2011-12-19

    Dec 19, 2011 ... the yolk color. This increase in shell thickness, shell weight and albumen weight were significant (P 0.05), though value was greater in probiotic treated birds. Egg production and quality are important determi- nations of the ...

  14. Studies on the effectiveness of oral pellet vaccine in improving egg production and egg quality in desi chicken.

    Science.gov (United States)

    Reetha, T Lurthu; Rajeswar, J Johnson; Harikrishnan, T J; Sukumar, K; Srinivasan, P; Kirubakaran, J John

    2016-08-01

    To study the effect of Newcastle disease (ND) oral pellet vaccine in egg production and egg quality in desi chicken. The study was conducted at Veterinary University Training and Research Centre, Tiruchirapalli, Tamil Nadu. A total of 48-day-old desi chicks obtained from a private hatchery in Namakkal, Tamil Nadu, were maintained under cage system of rearing up to 52 weeks of age as per standard management practices. All the 48 chicks were divided into six groups having eight chicks in each group were subjected to different treatment regimes. All the birds were challenged at 52 weeks of age with 0.5 ml dose of 10(4.0) egg infectious dose 50 virulent ND field virus. 10 eggs from each group were randomly collected during the last 3 days of 8 weeks interval period from 28 to 52 weeks of age and were used to measure the egg quality parameters. The production performance of each group was assessed at 4 weeks interval period from 25 to 52 weeks of age. In all the six treatment groups with respect to egg production, no significant difference (p≥0.05) was noticed from 25 to 52 weeks of age. Similarly, in egg weight, egg shape index and specific gravity, no significant difference (p≥0.05) was noticed from 28 to 52 weeks of age. From this study, it is concluded that the administration of ND oral pellet vaccine to desi chicken does not affect the egg production performance, egg weight, egg shape index, and specific gravity of egg.

  15. Effects of non-feed removal molting methods on egg quality traits in commercial brown egg laying hens in Turkey.

    Science.gov (United States)

    Petek, Metin; Gezen, S Sule; Alpay, Fazli; Cibik, Recep

    2008-08-01

    Non-feed removal molting programme in commercial brown laying hens and its influence on pre-molting, post-molting and end of cycle egg quality traits were investigated. Overall 54 birds were randomly divided into three treatment groups and each group was fed with one of the following diets during 10 days of molting period: (i) grain barley, (ii) alfalfa meal, or (iii) commercial layer ration (non-molted control group). Eggs obtained from groups in pre-molting, post-molting and end of cycle periods were examined for several quality performance traits such as egg weight, specific gravity, shape index, shell strength, shell thickness, eggshell weight, haugh unit, albumen index, yolk index and yolk color. Results indicated that non-feed removal molting programme based particularly on grain barley had positive effect on egg quality traits in laying hens. Notably, yolk color and haugh unit, which are considered as the most important quality parameters from the consumer point of view, were relatively improved in barley molted group.

  16. The influence of calcium and phosphorus levels on egg production, egg quality, tibia weight and 32P retention of layers

    International Nuclear Information System (INIS)

    Edwardly, Y.S.; Hendratno, C.; Yuyu Wahyu

    1979-01-01

    An experiment was conducted to study the influence of three levels of calcium and three levels of dietary phosphorus on egg production, egg quality, tibia weight and 32 P retention of layers. Calcium levels of 3.0; 3.5 and 4.0 percent were used and phosphorus contest of the diet fed were either 0.6; 0.9 or 1.2 percent.Egg production was highest with rations containing 3.5% calcium and 0.9% phosphorus. A significant increase in egg production was found at 0.9% levels of phosphorus compared to levels of 0.6 and 1.2%. Egg quality was increased significantly (p 32 P retention was low at the highest calcium level. (author)

  17. External control of the Drosophila melanogaster egg to imago development period by specific combinations of 3D low-frequency electric and magnetic fields.

    Science.gov (United States)

    Makarov, Vladimir I; Khmelinskii, Igor

    2016-01-01

    We report that the duration of the egg-to-imago development period of the Drosophila melanogaster, and the imago longevity, are both controllable by combinations of external 3-dimensional (3D) low-frequency electric and magnetic fields (LFEMFs). Both these periods may be reduced or increased by applying an appropriate configuration of external 3D LFEMFs. We report that the longevity of D. melanogaster imagoes correlates with the duration of the egg-to-imago development period of the respective eggs. We infer that metabolic processes in both eggs and imago are either accelerated (resulting in reduced time periods) or slowed down (resulting in increased time periods). We propose that external 3D LFEMFs induce electric currents in live systems as well as mechanical vibrations on sub-cell, whole-cell and cell-group levels. These external fields induce media polarization due to ionic motion and orientation of electric dipoles that could moderate the observed effects. We found that the longevity of D. melanogaster imagoes is affected by action of 3D LFEMFs on the respective eggs in the embryonic development period (EDP). We interpret this effect as resulting from changes in the regulation mechanism of metabolic processes in D. melanogaster eggs, inherited by the resulting imagoes. We also tested separate effects of either 3D electric or 3D magnetic fields, which were significantly weaker.

  18. Performance And Egg Quality Of Hens Fed Cocoa Husk Based Diet ...

    African Journals Online (AJOL)

    Lowman Brown hens, 37 - week - old, were used to study the effect of dietary inclusion of 10 and 20% cocoa husk meal (CHM) at the expense of maize on hen performance and egg quality. The trial was conducted for 10 weeks. Egg production (EP), egg weight (EW), egg mass (EM), feed consumption (FC), and feed ...

  19. Dietary fatty acid enrichment increases egg size and quality of yellow seahorse Hippocampus kuda.

    Science.gov (United States)

    Saavedra, M; Masdeu, M; Hale, P; Sibbons, C M; Holt, W V

    2014-02-01

    Seahorses populations in the wild have been declining and to restore them a better knowledge of seahorse reproduction is required. This study examines the effect of dietary quality on seahorse fecundity and egg quality. Two different diets were tested with Hippocampus kuda females: frozen mysis (control) and frozen mysis enriched with a liposome spray containing essential fatty acids. Diets were given to females (two groups of five) over a seven week period. After this period, males (fed the control diet) and females were paired and the eggs dropped by the females were collected. Fatty acid profile were analysed and eggs were counted and measured. Results showed that females fed on enriched mysis had larger eggs and that these had a higher content of total polyunsaturated fatty acids. The size of the egg was especially affected in the first spawn, where egg size for females fed the enriched diet was significantly higher than the egg size from control females. This effect was reduced in the following spawning where no significant differences were found. Egg size is an important quality descriptor as seahorse juveniles originating from smaller eggs and/or eggs of poor quality will have less chances of overcoming adverse conditions in the wild and consequently have lower survival and growth rates. This study shows that enriching frozen mysis with polyunsaturated fatty acids increases egg size and egg quality of H. kuda. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Studies on the effectiveness of oral pellet vaccine in improving egg production and egg quality in desi chicken

    Directory of Open Access Journals (Sweden)

    T. Lurthu Reetha

    2016-08-01

    Full Text Available Aim: To study the effect of Newcastle disease (ND oral pellet vaccine in egg production and egg quality in desi chicken. Materials and Methods: The study was conducted at Veterinary University Training and Research Centre, Tiruchirapalli, Tamil Nadu. A total of 48-day-old desi chicks obtained from a private hatchery in Namakkal, Tamil Nadu, were maintained under cage system of rearing up to 52 weeks of age as per standard management practices. All the 48 chicks were divided into six groups having eight chicks in each group were subjected to different treatment regimes. All the birds were challenged at 52 weeks of age with 0.5 ml dose of 104.0 egg infectious dose 50 virulent ND field virus. 10 eggs from each group were randomly collected during the last 3 days of 8 weeks interval period from 28 to 52 weeks of age and were used to measure the egg quality parameters. The production performance of each group was assessed at 4 weeks interval period from 25 to 52 weeks of age. Results: In all the six treatment groups with respect to egg production, no significant difference (p≥0.05 was noticed from 25 to 52 weeks of age. Similarly, in egg weight, egg shape index and specific gravity, no significant difference (p≥0.05 was noticed from 28 to 52 weeks of age. Conclusion: From this study, it is concluded that the administration of ND oral pellet vaccine to desi chicken does not affect the egg production performance, egg weight, egg shape index, and specific gravity of egg.

  1. Effect of superdosing phytase on productive performance and egg quality in laying hens

    Directory of Open Access Journals (Sweden)

    Jong Hyuk Kim

    2017-07-01

    Full Text Available Objective An experiment was conducted to determine the effect of superdosing phytase on productive performance and egg quality in laying hens. Methods A total of 200 42-wk-old Hy-Line Brown laying hens were allotted into 1 of 5 dietary treatments with 5 replicates consisting of 8 hens per replicate. The positive control (PC and negative control diets (NC were prepared based on the recommended P levels in layer diets. Supplemental phytase was added to the negative control diet at 10,000 (SD10, 20,000 (SD20, or 30,000 (SD30 fytase units (FTU/kg. Productive performance was summarized for 6 weeks from 42 weeks to 47 weeks of age. Egg quality was assessed from 4 eggs per replicate randomly collected at the conclusion of the experiment. Results The SD20 treatment had greater (p<0.05 hen-day egg production than PC, NC, and SD10 treatment groups. There was no difference in hen-day egg production between SD20 and SD30 treatment groups. However, SD30 treatment had greater (p<0.05 hen-day egg production than PC treatment, but showed no difference in hen-day egg production as compared to NC and SD10 treatment groups. However, egg weight, egg mass, feed intake, and feed conversion ratio were not affected by dietary treatments. Egg quality including eggshell strength, eggshell color, egg yolk color, and haugh unit was not influenced by dietary treatments. Conclusion Superdosing level of 20,000 FTU/kg phytase in diets has a positive effect on egg production rate, but no beneficial effect on egg quality in laying hens.

  2. Egg Qualities and Performance Characteristics of Laying Chicken in ...

    African Journals Online (AJOL)

    Meanwhile, hens in partitioned battery cage (control) gave the best results. However, except for the egg weight, the housing systems had no significant effect (p>0.05) on other egg quality parameters measured. Partitioned (conventional) battery cage is still the best system of housing laying chickens, although extended ...

  3. effects of extended period of storage and strain of layer on quality

    African Journals Online (AJOL)

    User

    strain of layers and extended storage length on internal and external qualities of chicken eggs. ... storage of egg on some important egg quality determinants like egg weight, albumen height and ... of information on the quality of eggs of these.

  4. Effects of egg shell quality and washing on Salmonella Infantis penetration.

    Science.gov (United States)

    Samiullah; Chousalkar, K K; Roberts, J R; Sexton, M; May, D; Kiermeier, A

    2013-07-15

    The vast majority of eggs in Australia are washed prior to packing to remove dirt and fecal material and to reduce the microbial contamination of the egg shell. The egg contents can be an ideal growth medium for microorganisms which can result in human illness if eggs are stored improperly and eaten raw or undercooked, and it is estimated that egg-related salmonellosis is costing Australia $44 million per year. Egg shell characteristics such as shell thickness, amount of cuticle present, and thickness of individual egg shell layers can affect the ease with which bacteria can penetrate the egg shell and washing could partially or completely remove the cuticle layer. The current study was conducted to investigate the effects of egg washing on cuticle cover and effects of egg shell quality and cuticle cover on Salmonella Infantis penetration of the egg shell. A higher incidence of unfavorable ultrastructural variables of the mammillary layer such as late fusion, type B bodies, type A bodies, poor cap quality, alignment, depression, erosion and cubics were recorded in Salmonella penetrated areas of egg shells. The influence of egg washing on the ability of Salmonella Infantis on the egg shell surface to enter the egg internal contents was also investigated using culture-based agar egg penetration and real-time qPCR based experiments. The results from the current study indicate that washing affected cuticle cover. There were no significant differences in Salmonella Infantis penetration of washed or unwashed eggs. Egg shell translucency may have effects on Salmonella Infantis penetration of the egg shell. The qPCR assay was more sensitive for detection of Salmonella Infantis from egg shell wash and internal contents than traditional microbiological methods. The agar egg and whole egg inoculation experiments indicated that Salmonella Infantis penetrated the egg shells. Egg washing not only can be highly effective at removing Salmonella Infantis from the egg shell surface

  5. Effect of dietary organic selenium and zinc on the internal egg quality of quail eggs for different periods and under different temperatures

    Directory of Open Access Journals (Sweden)

    IB Fernandez

    2011-03-01

    Full Text Available The objective of this study was to evaluate the internal quality of eggs of Japanese quails fed diets supplemented with chelated selenium and zinc. The experiment was carried out for 120 days, and 144 birds were divided in random blocks into four treatments (control; 0.3 ppm Se; 60 ppm Zn and 0.3 ppm Se + 60 ppm Zn. Ten, 14, 18, and 22 weeks after the beginning of lay, eggs were collected and stored under two different temperatures (environmental temperature or refrigeration and for 10, 20, and 30 days. Eggs were analyzed for: Haugh units (HU, albumen height (AH, yolk index (YI, and albumen index (AI. Parameters were only statistically influenced by the interaction between dietary treatment and storage time. It was concluded that the addition of organic Se and Zn influenced internal egg quality when eggs were stored up to 20 days, independently of storage temperature, suggesting that the combined supplementation of organic Se and Zn improve internal egg quality and extend egg shelf life.

  6. Evaluation of egg quality traits of endangered Nicobari fowl and its crosses under intensive and backyard system of Andaman and Nicobar Islands, India

    Directory of Open Access Journals (Sweden)

    N. C. Choudhuri

    2014-09-01

    Full Text Available Aim: In the present study, egg quality traits of endangered Nicobari fowl and its crosses (Nicorock and Nishibari were evaluated under intensive (deep litter as well as backyard system, in the regions of Andaman and Nicobar Islands, India. Materials and Methods: Fresh eggs (15 of all the three genetic groups of birds were collected randomly from institute farm (intensive system and from farmers’ field. The eggs were collected from adult birds (50 weeks of age 3 times with 5 in number in each time. All the genetic groups of birds were subjected to same husbandry practices. The eggs were subjected to external and internal quality parameters study. Results: The effect of genetic groups on egg weight was significant (p<0.05 for all the groups. Nicorock had significantly higher egg weight (g (56.79±0.77 in comparison to Nicobari (53.20±0.34 and Nishibari (48.98±0.22 under intensive system of management. Under backyard condition, the egg weight (g of Nicorock (48.60±1.04 was significantly higher than Nicobari but not with Nishibari. In general, the egg weight was found less under backyard system than intensive system that might be due to scavenging nature of birds. Egg length, egg width and shape index differed significantly (p<0.05 among the genetic groups. Yolk index of Nicorock was significantly (p<0.05 higher than Nishibari under intensive as well as backyard condition. The shell thickness varied significantly (p<0.05 among different genetic groups. Haugh unit of Nicorock was significantly (p<0.05 lower in comparison to Nicobari and Nishibari under intensive system, but did not vary significantly among genetic groups under backyard condition. Conclusions: The study revealed that there was a significant effect of genetic groups on different egg quality traits; both in intensive system and backyard condition.

  7. The table eggs and their quality in small-scale breeding

    Directory of Open Access Journals (Sweden)

    Mária Angelovičová

    2015-12-01

    Full Text Available The purpose this study was to investigate quality of the table eggs, their damage and soiling in various age of the laying hens (47-62 weeks during the second phase of the laying cycle. The object of the research was table eggs, egg white, egg shell, damage and soiling of the egg shell. The eggs were of the final laying hybrid ISA Brown reared in the non-cage system with deep litter and free range at small-scale breeding. In the breeding system with free range at small-scale conditions were secured requirements for laying hens in accordance with welfare principles. The eggs were collected each day at 4:00 pm. Weights of egg and egg shell samples of were measured on scales type KERN 440-35N. A white weight was calculated. Damage and soiling of eggs were investigated under the desk lamp lighting up to 100 W bulb. Statistical evaluation of the results was carried out in the program system SAS. From the existing conclusions of the various scientific and professional published works, it is known that both quality and safety are interrelated. In many works are the risk factors referred together as the quality standards. We found an important fact in assessing the trend of values of the egg shell weight, depending on the age of laying hens. Based on this fact, it can be assumed that the values of egg shell weight were not directly related to egg weight and egg white weight. It follows that the egg shell weight must be assessed comprehensively, and account must be taken of other factors. For the characteristics of the deformed egg shape has been one pc, representing 3.33% of the samples taken for analysis of eggs at 53 weeks of age of the total sampled eggs. In the following 56 weeks of age hens laying eggs there was not a deformed shape. The next subsequent sampling 59 weeks of age laying hens were recorded two pcs of eggs with deformed shape, i.e. 6.67%. At the last sampling of eggs at age 62 weeks, the number of eggs with deformed shape increased to

  8. QUALITY ASSESSMENT OF EGGS BROWN EGG LAYER HENS CREATION IN TWO SYSTEMS (CAGE AND NEST BED

    Directory of Open Access Journals (Sweden)

    J. Paula

    2015-02-01

    Full Text Available The research objective was to evaluate the effects on the eggs quality in two farming systems (bed + nest and cages for the laying hens brown-egg pullets were used 132 eggs, with six replicates for each treatment and 11 eggs each repetition, picked randomly from sheds both with 2000 DeKalb strain of laying hens housed in two sheds Brown, to create a nest bed + tubular feeders, the other one in cage system, all birds at approximately thirty weeks of age, which evaluated the following variables: weight of whole egg, egg weight without shell, albumen weight, yolk weight, yolk and albumen percentages, weight and thickness, color gem, the albumen pH and yolk, egg and classification according to RIISPOA. All collected data were analyzed by the statistical program SISVAR (2000 by Tukey test at 5% of probability. There were significant differences in the treatments studied in relation to the weight of whole egg, shelled egg weight, albumen%, skin thickness and coloration of the yolk, these results more significant to the breeding system in cages. However it can be concluded that in both systems when properly designed and managed can achieve good results and production performance of birds.

  9. Cortical reaction as an egg quality indicator in artificial reproduction of pikeperch, Sander lucioperca.

    Science.gov (United States)

    Zarski, Daniel; Krejszeff, Sławomir; Palińska, Katarzyna; Targońska, Katarzyna; Kupren, Krzysztof; Fontaine, Pascal; Kestemont, Patrick; Kucharczyk, Dariusz

    2012-01-01

    The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 females by artificial reproduction, in which hormonal stimulation with hCG was applied. Subsequently, each sample of eggs (separately from each female fish) was analysed. The analysis included observation of the cortical reaction and the process of egg swelling, and determination of the effect of temperature (12, 14 and 16°C) and the presence of spermatozoa on the cortical reaction. The results indicate that the cortical reaction in pikeperch eggs is quite violent, resulting in visible deformation of eggs between 3 and 5 min after activation. No effect of temperature or the presence of spermatozoa on the cortical reaction was observed. A strong correlation was recorded for the percentage of egg deformations observed and embryo survival rate. The described method of determination of pikeperch egg quality (based on egg deformation rate between 3 and 5 min after activation) may be highly useful, both in scientific research (where high-quality eggs are required) and in hatchery practice.

  10. Effects of lighting pattern and photoperiod on egg production and egg quality of a native chicken under free-range condition.

    Science.gov (United States)

    Geng, A L; Zhang, Y; Zhang, J; Wang, H H; Chu, Q; Liu, H G

    2018-04-14

    The paper aimed to study the effects of lighting pattern and photoperiod alone and in combination on egg production, egg quality in Beijing You Chicken (BYC). A total of 630 19-wk-old BYC laying hens were randomly allocated to 6 groups with 105 birds each, 3 replicates per group, reared in individually lit floor pens with separate outdoor areas. A 2 × 3 factorial experiment (2 lighting patterns: continuous and intermittent lighting; 3 photoperiods: 16, 14, 12 h) was arranged, including 16L:8D (6:00 to 22:00) for group 1; 12L:2D:4L:6D (6:00 to 18:00, 20:00 to 24:00) for group 2; 14L:10D (6:00 to 20:00) for group 3; 10L:2D:4L:8D (6:00 to 16:00, 18:00 to 22:00) for group 4; 12L:12D (6:00 to 18:00) for group 5, and 8L:4D:4L:8D (6:00 to 14:00, 18:00 to 22:00) for group 6, respectively. Egg production parameters were calculated for 22 to 43, 44 to 57, and 22 to 57 wk, and egg quality parameters were measured at the end of 37 and 57 wk. The results showed that the egg production of BYC was not significantly affected by lighting pattern, photoperiod alone, or in combination during 22 to 43 and 22 to 57 wk (P > 0.05), but average feed intake in 12 h groups was significantly higher than those in 14 and 16 h groups during 22 to 43 and 22 to 57 wk (P Egg mass and feed egg ratio were significantly affected by lighting pattern, photoperiod alone, and in combination during 44 to 57 wk (P Egg mass was significantly higher (P = 0.05) and feed egg ratio was significantly lower (P = 0.03) in continuous groups than in intermittent groups. There were significant effects for eggshell thickness, albumen height, haugh unit, and egg grade by lighting pattern alone (P egg production was not significantly affected by lighting pattern alone during 22 to 57 wk (P > 0.05), but the photoperiod significantly affected average feed intake (P egg production during 44 to 57 wk, and intermittent lighting is better for egg quality of the native bird at 37 wk; 3) 12 h lighting is enough for meeting

  11. Effect of Dietary Marine Microalgae ( Powder on Egg Production, Blood Lipid Profiles, Egg Quality, and Fatty Acid Composition of Egg Yolk in Layers

    Directory of Open Access Journals (Sweden)

    J. H. Park

    2015-03-01

    Full Text Available Two hundred and sixteen Institut de Sélection Animale (ISA brown layers (40 wks of age were studied for 6 wks to examine the effect of microalgae powder (MAP on egg production, egg quality, blood lipid profile, and fatty acid concentration of egg yolk. Dietary treatments were as follows: i CON (basal diet, ii 0.5% MAP (CON+0.5% Schizochytrium powder, and iii 1.0% MAP (CON+1.0% Schizochytrium powder. From 44 to 46 wks, egg production was higher in 1.0% MAP treatment than in control treatment (linear, p = 0.034; however, there was no difference on the egg production from 40 to 43 wks (p>0.05. Serum triglyceride and total cholesterol were significantly reduced in the groups fed with MAP, compared to those in groups fed with control diets (Quadratic, p = 0.034 and p = 0.039, respectively. Inclusion of 0.5% MAP in the diet of layers improved egg yolk color, compared with hens fed with basal diet at 46 wks (quadratic, p = 0.044. Eggshell thickness was linearly increased in MAP-fed treatments at 46th wk (p<0.05. Concentration of yolk docosahexaenoic acid (DHA; C22:6n-3 was increased in treatment groups fed with MAP (linear, p<0.05. The n-6 fatty acids, n-6/n-3 fatty acid, and unsaturated fatty acid/saturated fatty acid were decreased in treatment groups fed with MAP (linear, p<0.05. These results suggest that MAP improved the egg production and egg quality, and may affect serum lipid metabolites in the layers. In addition, MAP increases yolk DHA levels, and deceases n-6/n-3 fatty acid ratio.

  12. Influence of grinding size of the main cereal of the diet on egg production and eggs quality of brown egg laying hens from 33 to 65 weeks of age.

    Science.gov (United States)

    Herrera, J; Saldaña, B; Cámara, L; Berrocoso, J D; Mateos, G G

    2018-04-17

    The influence of grinding size of the main cereal of the diet on production and egg quality traits was studied in brown hens from 33 to 65 wk of age. The experiment was completely randomized with 6 treatments arranged as a 3 × 2 factorial with 3 main cereals (barley, corn, and wheat) and 2 grinding size of the cereal (6 and 10 mm screen). Each treatment was replicated 11 times (10 hens/replicate). Diets were isonutritive and contained 2,740 kcal/kg AMEn and 16.8% CP. Egg production, ADFI, egg weight, and feed conversion ratio (FCR) were determined by period (4 wk) and for the entire experiment. Egg quality traits (percentage of undergrades, Haugh units, thickness, strength, color of the shell, and proportion of albumen, yolk, and shell) were measured also by period. No interactions between main cereal and grinding size of the main cereal of the diet were observed for any of the traits studied. Feed intake, egg production, and BW gain were not affected by diet or grinding size. Eggs were heavier (P < 0.01) in hens fed barley than in hens fed corn or wheat, probably because of the higher fat content of the barley diets. Also, FCR tended to improve in hens fed barley compared with hens fed corn or wheat (P = 0.07). Diet did not affect any of the egg quality traits studied. In summary, barley and wheat conveniently supplemented with enzymes, can be used in substitution of corn at levels of up to 55% in diets for laying hens, without any adverse effect on egg production or egg quality traits. Moreover, the substitution of corn by a combination of barley and supplemental fat increased egg size. Consequently, the inclusion of one or other cereal in the diet will depend primarily on their relative cost. Within the range studied, screen size (6 vs. 10 mm) of the cereal had limited effects on hen production.

  13. Effect of Some Egg Quality Traits on Hatching Results in Brown Pure Lines

    Directory of Open Access Journals (Sweden)

    İsmail Durmuş

    2016-09-01

    Full Text Available This study was conducted to determine the influence of eggshell thickness, albumen height, yolk height and haugh unit on hatching results. A total of 746 eggs, which were obtained and evaluated for egg quality parameters from 100 brown pure line hens of 42 weeks of age, were used. Eggs were collected from each hen for 12 days and individual incubation was carried out. Eggs were grouped based on quality parameters and evaluated accordingly. The findings suggested that hatchability, hatchability of fertile eggs, early, mid and late embryonic mortality did not differ in terms of haugh unit, albumen height, eggshell thickness and yolk height groups. However, early embryonic mortality was found different between the yolk height groups. No relationship was determined among albumen height, haugh unit, eggshell thickness and hatching results. There was no relation between yolk height and hatchability of fertile eggs, hatchability, mid and late embryonic mortalities but was a positive correlation with early embryonic mortality. The results of the present study demonstrate that egg quality parameters studied here had no influence on hatching results except that early embryonic mortality increased with the yolk height.

  14. Nutritional modulation of health, egg quality and environmental pollution of the layers.

    Science.gov (United States)

    Wang, Jing; Yue, Hongyuan; Wu, Shugeng; Zhang, Haijun; Qi, Guanghai

    2017-06-01

    World egg production and consumption have been increasing for the past decades. Traditional strategies in poultry nutrition have made vital contributions to this great growth in quantity. However, current global issues should be considered in modern egg production such as growing populations and food security, food safety and quality, limited resources and environmental problems. The development of knowledge of poultry nutrition and modern biotechnology provides novel nutritional approaches to closely fit the requirement of pullets and laying hens, which will consequently decrease the nutrition excretion and maintain the lower cost of feed. Nutrition has also been widely accepted as a strategy to influence health and diseases of laying hens. The maintenance of good health is an important prerequisite for improving productivity and egg quality. In addition, there are many measures and strategies for minimizing the incidence of egg defects and providing a choice of lifestyle to enhance human health. This paper reviews current research progress on developing innovative technologies and strategies to maximize animal health and performance, improve the quality of egg products and minimize pollution caused by poultry production.

  15. Nutritional modulation of health, egg quality and environmental pollution of the layers

    Directory of Open Access Journals (Sweden)

    Jing Wang

    2017-06-01

    Full Text Available World egg production and consumption have been increasing for the past decades. Traditional strategies in poultry nutrition have made vital contributions to this great growth in quantity. However, current global issues should be considered in modern egg production such as growing populations and food security, food safety and quality, limited resources and environmental problems. The development of knowledge of poultry nutrition and modern biotechnology provides novel nutritional approaches to closely fit the requirement of pullets and laying hens, which will consequently decrease the nutrition emissions and maintain the lower cost of feed. Nutrition has also been widely accepted as a strategy to influence health and diseases of laying hens. The maintenance of good health is an important prerequisite for improving productivity and egg quality. In addition, there are many measures and strategies for minimizing the incidence of egg defects and providing a choice of lifestyle to enhance human health. This paper reviews current research progress on developing innovative technologies and strategies to maximize animal health and performance, improve the quality of egg products and minimize pollution caused by poultry production.

  16. Comparison of the effects of dietary supplementation of flavonoids on laying hen performance, egg quality and egg nutrient profile.

    Science.gov (United States)

    Iskender, H; Yenice, G; Dokumacioglu, E; Kaynar, O; Hayirli, A; Kaya, A

    2017-10-01

    1. The aim of this experiment was to compare the effects of dietary supplementation of hesperidin, naringin and quercetin on laying hen performance, egg quality and egg yolk lipid and protein profiles. 2. A total of 96 Lohmann White laying hens weighing an average of 1500 g at 28 weeks of age were randomly assigned to a basal diet and the basal diet supplemented (0.5 g/kg) with either hesperidin, naringin or quercetin. Each treatment was replicated in 6 cages in an 8-week experimental period. Data were analysed using one-way analysis of variance. 3. None of the dietary flavonoids affected laying performance and eggshell quality. Hesperidin and quercetin supplementations decreased albumen and yolk indexes. 4. As compared to the control group, egg yolk cholesterol content decreased and egg yolk protein content increased in response to dietary hesperidin and quercetin supplementation. The mean egg yolk cholesterol (mg/g) and protein (g/100 g) contents were 10.08/14.28, 16.12/14.08, 14.75/15.04 and 15.15/14.85 for the control group and groups supplemented with naringin, hesperidin and quercetin, respectively. 5. Egg yolk lipid and protein profiles were variable. 6. In conclusion, dietary supplementation of hesperidin or quercetin could be used in the diets during the early laying period to reduce egg yolk cholesterol and increase egg yolk protein, which may be attractive to consumers.

  17. Correlation of quality measurements to visible-near infrared spectra of pasteurized egg

    Science.gov (United States)

    A twelve week study was conducted on the egg albumen from both pasteurized and non-pasteurized shell eggs using visible-near infrared spectroscopy. Correlation of the chemical changes detected in the spectra to the measurement of Haugh units (measure of interior egg quality) was carried out using ch...

  18. Effects of balanced dietary protein levels on egg production and egg quality parameters of individual commercial layers.

    Science.gov (United States)

    Shim, M Y; Song, E; Billard, L; Aggrey, S E; Pesti, G M; Sodsee, P

    2013-10-01

    The effects of a series of balanced dietary protein levels on egg production and egg quality parameters of laying hens from 18 through 74 wk of age were investigated. One hundred forty-four pullets (Bovans) were randomly assigned to individual cages with separate feeders including 3 different protein level series of isocaloric diets. Diets were separated into 4 phases of 18-22, 23-32, 33-44, and 45-74 wk of age. The high protein (H) series contained 21.62, 19.05, 16.32, and 16.05% CP, respectively. Medium protein (M) and low protein (L) series were 2 and 4% lower in balanced dietary protein. The results clearly demonstrated that the balanced dietary protein level was a limiting factor for BW, ADFI, egg weight, hen day egg production (HDEP), and feed per kilogram of eggs. Feeding with the L series resulted in lower ADFI and HDEP (90.33% peak production) and more feed per kilogram of eggs compared with the H or M series (HDEP; 93.23 and 95.68% peak production, monthly basis). Egg weight responded in a linear manner to balanced dietary protein level (58.78, 55.94, and 52.73 g for H, M, and L, respectively). Feed intake of all hens, but especially those in the L series, increased considerably after wk 54 when the temperature of the house decreased due to winter conditions. Thus, hens fed the L series seemed particularly dependent on house temperature to maintain BW, ADFI, and HDEP. For egg quality parameters, percent yolk, Haugh units, and egg specific gravity were similar regardless of diets. Haugh units were found to be greatly affected by the variation of housing temperature (P = 0.025). Maximum performance cannot always be expected to lead to maximum profits. Contrary to the idea of a daily amino acid requirement for maximum performance, these results may be used to determine profit-maximizing levels of balanced dietary protein based on the cost of protein and returns from different possible protein levels that may be fed.

  19. Regulation of egg quality and lipids metabolism by Zinc Oxide Nanoparticles.

    Science.gov (United States)

    Zhao, Yong; Li, Lan; Zhang, Peng-Fei; Liu, Xin-Qi; Zhang, Wei-Dong; Ding, Zhao-Peng; Wang, Shi-Wen; Shen, Wei; Min, Ling-Jiang; Hao, Zhi-Hui

    2016-04-01

    This investigation was designed to explore the effects of Zinc Oxide Nanoparticles (ZnO NP) on egg quality and the mechanism of decreasing of yolk lipids. Different concentration of ZnO NP and ZnSO4 were used to treat hens for 24 weeks. The body weight and egg laying frequency were recorded and analyzed. Albumen height, Haugh unit, and yolk color score were analyzed by an Egg Multi Tester. Breaking strength was determined by an Egg Force Reader. Egg shell thickness was measured using an Egg Shell Thickness Gouge. Shell color was detected by a spectrophotometer. Egg shape index was measured by Egg Form Coefficient Measuring Instrument. Albumen and yolk protein was determined by the Kjeldahl method. Amino acids were determined by an amino acids analyzer. Trace elements Zn, Fe, Cu, and P (mg/kg wet mass) were determined in digested solutions using Inductively Coupled Plasma-Optical Emission Spectrometry. TC and TG were measured using commercial analytical kits. Yolk triglyceride, total cholesterol, pancreatic lipase, and phospholipids were determined by appropriate kits. β-carotene was determined by spectrophotometry. Lipid metabolism was also investigated with liver, plasma, and ovary samples. ZnO NP did not change the body weight of hens during the treatment period. ZnO NP slowed down egg laying frequency at the beginning of egg laying period but not at later time. ZnO NP did not affect egg protein or water contents, slightly decreased egg physical parameters (12 to 30%) and trace elements (20 to 35%) after 24 weeks treatment. However, yolk lipids content were significantly decreased by ZnO NP (20 to 35%). The mechanism of Zinc oxide nanoparticles decreasing yolk lipids was that they decreased the synthesis of lipids and increased lipid digestion. These data suggested ZnO NP affected egg quality and specifically regulated lipids metabolism in hens through altering the function of hen's ovary and liver. © 2016 Poultry Science Association Inc.

  20. Effects of dietary oil sources on egg quality, fatty acid composition of ...

    African Journals Online (AJOL)

    The results of this study demonstrated that olive oil improved egg weight and egg shell quality compared to the other oils tested; fish and soyabean oil increased the omega-3 fatty acid level of egg yolk, and soyabean oil had positive effects on serum lipid concentrations. Incorporation of these oils into the diets of Japanese ...

  1. Egg quality of quails fed low methionine diet supplemented with betaine

    Science.gov (United States)

    Ratriyanto, A.; Indreswari, R.; Dewanti, R.; Wahyuningsih, S.

    2018-03-01

    This experiment investigated the effect of betaine supplementation to low methionine diet on egg quality of quails. A total of 340 laying quails (Coturnix coturnix japonica) was divided into 4 dietary treatments with 5 replicates of 17 quails each. The experiment was assigned in a completely randomized design. The four dietary treatments were the low methionine diet (0.3% methionine) without betaine supplementation and the low methionine diet supplemented with 0.07, 0.14, and 0.21% betaine. The experimental diets were applied for 8 weeks and the egg quality traits were measured at the age of 16 and 20 weeks. The data were subjected to analysis of variance, and when the treatment indicated significant effect, it was continued to orthogonal polynomial test to determine the optimum level of betaine. Increasing dietary levels of betaine increased the fat content of the egg with the linear regression of y = 11.0949 + 4.1914x (R2 = 0.18). However, supplementation of betaine did not affect protein content, yolk, albumen, and eggshell percentage. It can be concluded that betaine supplementation up to 0.21% to low methionine diet only had little effect in improving the quality traits of quail eggs.

  2. Effect of Dietary Crude Protein and Methionine on Egg Production and Egg Quality of Laying Hens During Phase II

    Directory of Open Access Journals (Sweden)

    H Mohammadi Emarat

    2012-02-01

    Full Text Available An experiment was conducted to evaluate the effect of dietary crude protein and methionine levels on quality and quantity of egg production. Fifteen diets formulated with 3 levels of protein (13, 14 and 15% and 5 levels of methionine (0.25, 0.28, 0.31, 0.34 and 0.37% and fed to 420 birds in a 3×5 factorial arrangement. Each diet was randomly fed to 4 replicates of 7 birds each and fed for 3 periods of 4 weeks (50-62wks of age each. Egg number and mortality was recorded daily, whereas feed consumption determined at the end of each period. The increased in dietary protein significantly increased egg production from 54 to 59.4 %. Egg weight, egg mass and feed intake increased by 1.7 g, 3.4 g, and 2.8 g, respectively during the whole experimental period. As the dietary protein increased, feed conversion, egg component (as a percent of whale egg and egg albumin percent were improved. However, the egg breaking, specific gravity and eggshell were significantly decreased with increased dietary protein. The egg yolk percent was not influenced by dietary protein levels. The increased in dietary methionine from 0.25% to 0.37% caused the overall egg production, egg weight, egg mass, feed intake and egg component to improve by about 8.2%, 4g, 6.6g, 8.7g, and 6.0g, respectively. Feed conversion, specific gravity, egg breakage, egg shell, and egg yolk and albumin percent were not influenced by dietary methionine levels.

  3. Exterior egg quality as affected by enrichment resources layout in furnished laying-hen cages.

    Science.gov (United States)

    Li, Xiang; Chen, Donghua; Meng, Fanyu; Su, Yingying; Wang, Lisha; Zhang, Runxiang; Li, Jianhong; Bao, Jun

    2017-10-01

    This study aimed to investigate the effects of enrichment resources (a perch, dustbath, and nest) layout in furnished laying-hen cages (FC) on exterior quality of eggs. One hundred and sixty-eight (168) Hy-Line Brown laying hens at 16 weeks of age were randomly distributed to four treatments: small furnished cages (SFC), medium furnished cages type I (MFC-I), medium furnished cages type II (MFC-II), and medium furnished cages type III (MFC-III). Each treatment had 4 replicates or cages with 6 hens for SFC (24 birds for each SFC) and 12 hen/cage for MFC-I, -II, and -III (48 birds for each MFC-I, -II and -III). Following a 2-week acclimation, data collection started at 18 weeks of age and continued till 52 weeks of age. Dirtiness of egg surface or cracked shell as indicators of the exterior egg quality were recorded each week. The results showed that the proportion of cracked or dirty eggs was significantly affected by the FC type (p<0.01) in that the highest proportion of cracked or dirty eggs was found in MFC-I and the lowest proportion of dirty eggs in SFC. The results of this showed that furnished cage types affected both dirty eggs and cracked eggs (p<0.01). The results also indicated that not nest but dustbath lead to more dirty eggs. Only MFC-I had higher dirty eggs at nest than other FC (p< 0.01). The results of dirty eggs in MFC-I and MFC-II compared with SFC and MFC-III seemed suggest that a low position of dustbath led to more dirty eggs. SFC design affected exterior egg quality and the low position of dustbath in FC resulted in higher proportion of dirty eggs.

  4. Ozone treatment of shell eggs to preserve functional quality and enhance shelf life during storage.

    Science.gov (United States)

    Yüceer, Muhammed; Aday, Mehmet Seçkin; Caner, Cengiz

    2016-06-01

    Eggs have long been recognised as a source of high-quality proteins. Many methods exist to extend shelf life of food and one of them is ozone treatment, which is an emerging technology for disinfecting surfaces in the food industry. This study aimed to extend the shelf life of fresh eggs using gaseous ozone treatments at concentrations of 2, 4 and 6 ppm with exposure times of 2 and 5 min during storage for 6 weeks at 24 °C. The effect of the treatments on interior quality and functional properties of eggs is also reported. Ozone concentration and exposure time significantly affected the Haugh unit (HU), yolk index, albumen pH, relative whipping capacity (RWC), and albumen viscosity of eggs during the storage. Control eggs had the highest albumen pH and lowest albumen viscosity. Attributes such as albumen pH and RWC of eggs exposed to ozone treatments were better than the control samples. The measurement results showed that ozone concentration at 6 ppm and exposure time of 5 min can be applied to fresh eggs and extend shelf life up to 6 weeks at 24 °C storage period. Ozone treatments helped to maintain egg quality for a longer time. Ozone concentrations at 2 and 4 ppm showed promising results in maintaining internal quality and functional properties of fresh eggs during storage. Ozone at high concentration (6 ppm) caused a detrimental effect on eggshell quality. As a result, this study demonstrated that ozone treatments of 2, and especially 4 and 6 ppm concentration maintained eggshell quality during the storage. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  5. Determining Tocotrienol Rich Fraction Effects on Laying, Mortality and Egg Quality Parameters

    Directory of Open Access Journals (Sweden)

    Mardhati, M.

    2016-12-01

    Full Text Available The public interest and awareness in healthier lifestyle has increased the demand of functional food, nutraceuticals and designer foods, which has resulted in the creation of new foods in marketplace, for instance designer and specialty eggs. A feeding trial was carried out to evaluate the laying performance of H&N layer chickens fed diets supplemented with tocotrienol rich fraction (TRF, as well as to quantify tocopherol and tocotrienol accumulation in eggs and their effect on egg quality. A total of 300 H&N laying hens (17-wk old were randomly allocated to 50 battery cages with 6 birds per cage. The birds were assigned equally to 6 different treatment diets; commercial diet (F1, corn-soy based diet (F2, corn-soy based diet added with 25ppm (F3, 50ppm (F4, 75ppm (F5 and 100ppm (F6 of tocotrienol rich fraction (TRF. Egg production and egg quality measurements were not significantly different (P>0.05 between treatments. Egg yolk from hens fed TRF supplemented diets contained more tocotrienol compared to those fed corn-soy based diet (P0.05. As feeding time progressed, the tocotrienol content tended to accumulate in the egg yolks (P<0.05.

  6. Moulting patterns of Alabio and Mojosari ducks and their relation on blood lipids (tryglycerides, egg production and egg quality

    Directory of Open Access Journals (Sweden)

    Maijon Purba

    2005-06-01

    Full Text Available Moulting is a biological condition that can happen in poultry. It is resulted from the complex interaction which involves the function of thyroxine hormone. Moulting can reduce or even stop the egg production. A study was conducted to observe the moulting patterns of local ducks (Alabio and Mojosari and to determine the relation of moulting with blood lipids (triglycerides, egg production and quality. Each breed consisted of ten female dukcs were observed for moulting pattern, blood triglycerides, egg production and quality. Fourty ducks were used for simulation of egg production. Data from moulting patterns,egg production and quality were analyzed using t-based on Least Square means with Statistical Analysis System. The relation of breeds and moulting patterns with triglycerides were analyzed using Analysis of Variance (ANOVA for a completely randomized design in a factorial arrangement of 2x2. The main factor was kind of breeds, while the subfactor was the period of moulting, before and during moulting. There were not interaction in every variables between both factors. The average moulting periode of Alabio was significantly (P<0.05 shorter than that of Mojosari (69 vs 76 days. There were 40% of Alabio ducks moulting for 61-70 days, while 40% of Mojosari ducks moulting for 71-80 days. Egg production of Alabio ducks before and after moulting were higher than those Mojosari ducks. The triglycerides content of Alabio and Mojosari ducks was decreased during moulting, in Alabio ducks they were 32.02 and 27.64 μg/ml before and during moulting, while in Mojosari ducks they were 32.83 and 29.32 μg/ml respectively. Egg weight, albumin weight, yolk weight, and haugh unit of the two breeds increased after moulting, while yolk colour decreased. The average yolk colour of Alabio ducks before and after moulting were 6.90 and 5.11, while in Mojosari ducks they were 7.90 and 4.60 respectively.

  7. Mother-Offspring Relations: Prey Quality and Maternal Size Affect Egg Size of an Acariphagous Lady Beetle in Culture

    Directory of Open Access Journals (Sweden)

    Eric W. Riddick

    2012-01-01

    Full Text Available We investigated mother-offspring relations in a lady beetle Stethorus punctillum Weise that utilizes spider mites as prey. Our objectives were to determine if (1 prey quality affects egg size, (2 maternal size correlates with egg size, and (3 egg size affects hatching success. We fed predators spider mites Tetranychus urticae Koch from lima bean Phaseolus lunatus L. foliage in the laboratory. Mothers of unknown body size offered high rather than low quality spider mites since birth produced larger eggs. Mothers of known body size offered only high quality spider mites, produced eggs of variable size, but mean egg size correlated positively with hind femur length. Mothers laid their eggs singly, rather than in batches, and eggs were large relative to femur size. Egg size did not affect hatch success; mean hatch rate exceeded 95% regardless of egg size. In conclusion, the quality of prey consumed by S. punctillum mothers while in the larval stage can affect their size as adults and, consequently, the size of their eggs. The behavior of laying eggs singly, the positive relationship between maternal size and mean egg size, and the high rate of egg hatch suggest that S. punctillum mothers invest heavily in offspring.

  8. Effect of Dietary Sodium Nitrate Consumption on Egg Production, Egg Quality Characteristics and Some Blood Indices in Native Hens of West Azarbaijan Province

    Directory of Open Access Journals (Sweden)

    H. Safary

    2012-11-01

    Full Text Available The aim of the study was to evaluate the effect of sodium nitrate consumption on egg quality and quantity, and some blood parameters of native breeder hens of West Azerbaijan province. One hundred native hens were used from wk 25 to 32 of age. These birds were divided into two groups. One group was fed the control diet (CD but the other fed the same diet supplemented with 4.2 g/kg sodium nitrate (ND. After 2 wks of adaptation, eggs were collected daily and egg mass and egg production were measured weekly for five weeks. To assess the egg quality parameters, two eggs from each replicate pen were collected for three consecutive days each week. At the end of experimental period (wk 32 of age, blood samples of 5 birds per replicate were collected from the wing vein into anticoagulant tubes. Dietary sodium nitrate didn’t affect the egg production, shell stiffness, shell thickness and Haugh unit (p>0.05 but it decreased the both egg production and egg mass during the last three weeks (wks 30, 31 and 32 (p0.05. No effect of time or treatment×time were observed for shell stiffness (p>0.05. Over time, shell thickness was decreased while Haugh unit increased (p0.05. Sodium nitrite decreased both the TAC and TC at wk 32 of age (p<0.001. It was concluded that the lower body antioxidant capacity of nitrate fed birds resulted in the lower performance (egg weight, egg production and egg mass.

  9. Evaluation of Egg Quality Traits of Local Chickens in the Humid ...

    African Journals Online (AJOL)

    Four hundred and thirty six (436) eggs laid by seven genotypes of local chickens within 56 days were used to evaluate egg quality traits. The genotypes evaluated were the homozygous naked neck (Na/Na) and frizzle (F/F), heterozygous naked neck (Na/na) and frizzle (F/na), reciprocal crosses between naked neck and ...

  10. [Identification of special quality eggs with NIR spectroscopy technology based on symbol entropy feature extraction method].

    Science.gov (United States)

    Zhao, Yong; Hong, Wen-Xue

    2011-11-01

    Fast, nondestructive and accurate identification of special quality eggs is an urgent problem. The present paper proposed a new feature extraction method based on symbol entropy to identify near infrared spectroscopy of special quality eggs. The authors selected normal eggs, free range eggs, selenium-enriched eggs and zinc-enriched eggs as research objects and measured the near-infrared diffuse reflectance spectra in the range of 12 000-4 000 cm(-1). Raw spectra were symbolically represented with aggregation approximation algorithm and symbolic entropy was extracted as feature vector. An error-correcting output codes multiclass support vector machine classifier was designed to identify the spectrum. Symbolic entropy feature is robust when parameter changed and the highest recognition rate reaches up to 100%. The results show that the identification method of special quality eggs using near-infrared is feasible and the symbol entropy can be used as a new feature extraction method of near-infrared spectra.

  11. Egg Intake and Dietary Quality among Overweight and Obese Mexican-American Postpartum Women

    Directory of Open Access Journals (Sweden)

    Sonia Vega-López

    2015-10-01

    Full Text Available Despite their low cost and high nutrient density, the contribution of eggs to nutrient intake and dietary quality among Mexican-American postpartum women has not been evaluated. Nutrient intake and dietary quality, as assessed by the Healthy Eating Index 2010 (HEI-2010, were measured in habitually sedentary overweight/obese (body mass index (BMI = 29.7 ± 3.5 kg/m2 Mexican-American postpartum women (28 ± 6 years and compared between egg consumers (n = 82; any egg intake reported in at least one of three 24-h dietary recalls and non-consumers (n = 57. Egg consumers had greater intake of energy (+808 kJ (193 kcal or 14%; p = 0.033, protein (+9 g or 17%; p = 0.031, total fat (+9 g or 19%; p = 0.039, monounsaturated fat (+4 g or 24%; p = 0.020, and several micronutrients than non-consumers. Regarding HEI-2010 scores, egg consumers had a greater total protein foods score than non-consumers (4.7 ± 0.7 vs. 4.3 ± 1.0; p = 0.004, and trends for greater total fruit (2.4 ± 1.8 vs. 1.9 ± 1.7; p = 0.070 and the total composite HEI-2010 score (56.4 ± 12.6 vs. 52.3 ± 14.4; p = 0.082. Findings suggest that egg intake could contribute to greater nutrient intake and improved dietary quality among postpartum Mexican-American women. Because of greater energy intake among egg consumers, recommendations for overweight/obese individuals should include avoiding excessive energy intake and incorporating eggs to a nutrient-dense, fiber-rich dietary pattern.

  12. Turmeric root and annato seed in second-cycle layer diets: performance and egg quality

    Directory of Open Access Journals (Sweden)

    C Laganá

    2011-09-01

    Full Text Available The objective of this study was to evaluate the effects of the inclusion of annato (Bixa orellana L. and turmeric (Turmeric longa L. in layer feeds on live performance, egg quality, and yolk pigmentation and depigmentation time. A number of 144 layers were distributed in a completely randomized experimental design, with four treatments with six replicates of six birds each. In the basal diet, sorghum replaced 50% of corn, and was supplemented or not with natural pigments to composse the following treatments: Control (0% pigments, AS (2.0% annato, TR (2% turmeric and ASTR (1% annato and 1% turmeric. Egg weight (g, egg production (%, egg mass (%, feed intake (g, feed conversion ratio (kg/dz and kg/kg and mortality were evaluated. The following egg quality parameters were evaluated: specific gravity (SG; yolk, albumen, and eggshell percentages, and yolk color. The treatments did not influence layer performance or egg quality parameters, except for egg production and yolk color. The dietary inclusion of 1% turmeric root and 1% annato seed promoted higher egg production. Diets containing annato resulted in more saturated, more intense, and redder yolk color, with increasing pigment deposition after day 10, with maximum values obtained on day 28. Dried turmeric root did not promote good yolk pigmentation, resulting in higher presence of white in the yolk, which was stabilized on day 4. Three days after pigments were withdrawn from the feeds, yolk color faded in the treatments with annato inclusion.

  13. Egg quality in layers housed in different production systems and submitted to two environmental conditions

    Directory of Open Access Journals (Sweden)

    JAD Barbosa Filho

    2006-03-01

    Full Text Available The production system using cages is a highly polemical issue in Europe, because of the space restriction imposed to laying hens. It is considered that the cage system might compromise important comfort movements, welfare and egg quality. This study evaluated egg quality and welfare of two strains of hens housed in a conventional system (battery laying cages or litter system with nest and perches, and submitted to heat stress or comfort conditions. Two groups of 20 birds (10 Hy-line W36 and 10 Hy-line Brown were submitted to two environmental conditions (26°C and 60% RH or 35°C and 70% RH and two housing systems (cages or litter in the early production phase. Egg quality was analyzed based on egg weight, eggshell thickness, specific gravity, and Haugh units. Yolk and shell contamination by Salmonella sp was also assessed. A significant (p<0.05 reduction in quality parameters was observed in eggs produced by laying hens under heat stress, mainly in the birds housed in cages.

  14. Effect of ultrasonic treatment on reduction of Esherichia coli ATCC 25922 and egg quality parameters in experimentally contaminated hens' shell eggs.

    Science.gov (United States)

    Sert, Durmus; Aygun, Ali; Torlak, Emrah; Mercan, Emin

    2013-09-01

    In this study, hen eggs which were experimentally contaminated with Esherichia coli ATCC 25922 were used. Contaminated eggs were washed statically (S5 to S30; 0 kHz) and by ultrasonic waves (U5 to U30; 35 kHz) for given applications of time (5, 15 and 30 min), then the eggs were stored at 22°C for 14 days. Depending on the time of ultrasonic application, a significant increase in egg shell strength (P eggs which were washed by ultrasonic waves. Yolk width values of ultrasonic washed eggs diminished. E. coli was completely removed by 30 min of ultrasonic application. During storage E. coli growth was not detected on the eggs which were washed by ultrasonic waves except the eggs in U5 group (2.04 log CFU eggshell⁻¹) on the first day of storage. Depending on the time of ultrasonic application a significant increase in egg quality parameters (shell strength, albumen height, Haugh units, and yolk height) were observed. The application of ultrasound led to a significant reduction in E. coli numbers on egg shells. © 2013 Society of Chemical Industry.

  15. The effect of quantity of added eggs on whole meal pasta quality

    Directory of Open Access Journals (Sweden)

    Filipović Jelena S.

    2014-01-01

    Full Text Available This paper investigates the convenience of different chemical and rheological methods for determining the quality of two spelt cultivars for pasta making and the influence of egg quantity on whole meal pasta quality. Post-hoc Tukey’s HSD test at 95% confidence limit has been calculated to show significant differences between different samples. Score analysis is being useful tool for accessing the effect of eggs to spelt pasta quality, and this analysis proved that though lower scores (0.270 for rheological characteristics experienced with cultivar Eco, the addition of eggs is positively contributing to the spelt pasta quality yielding the best score for pasta (0.75, contrary to the cultivar Austria attributed with superior rheology scores. Whole meal spelt is characterised by lower Ca, but higher Fe and Mn content in comparison to bread wheat. In comparison with common pasta, spelt is a suitable raw material for a new product with improved functional properties at the market.[Projekat Ministarstva nauke Republike Srbije, br. TRI 46005 i br. TR 31055

  16. Limestone and oyster shell for brown layers in their second egg production cycle

    Directory of Open Access Journals (Sweden)

    CC Pizzolante

    2011-06-01

    Full Text Available This study aimed at evaluating the effect of dietary calcium levels and the replacement of calcium sources with different particle size compositions on the performance and egg quality of brown layers in their second egg production cycle. A randomized block experimental design was applied with 12 treatments in a 3x4 factorial arrangement: three calcium levels (2.6, 3.2, 3.8 % and four combinations of calcium sources (1- 100% fine limestone (FL, 2- 50% FL + 50% coarse limestone (CL, 3- 50% FL and 50% oyster shell (OS, 4- 50% FL and 25% CL+ 25 %OS, with six replicates of eight birds each. Calcium sources were analyzed for geometric mean diameter (GMD and in-vitro solubility. The following performance and egg quality parameters were evaluated: egg weight (EW, g, egg production (% Eggs, egg mass (EM %, feed intake (FI g, feed conversion ratio (FCR kg/dz and FCR kg/kg, mortality (% Mort., specific egg gravity (SG, percentages of yolk (Y%, albumen (Alb% and eggshell (ES%, eggshell thickness (EST, eggshell breaking strength (BS, eggshell weight per surface area (EWSA, Haugh unit (HU, yolk index (YI and yolk color. Performance and internal egg quality were not affected by the treatments (p>0.05. Blocks had a significant effect on (p<0.05 FI and FCR (kg/dz and kg/kg. Treatments significantly influenced external egg quality, which improved as dietary calcium levels increases and when up to 50% fine limestone was replaced by combinations of coarse limestone with oyster shell.

  17. Determining Tocotrienol Rich Fraction Effects on Laying, Mortality and Egg Quality Parameters

    OpenAIRE

    Mardhati, M.; Farah Nurshahida, M.S.; Osman, A.; Wan Nooraida, W.M.

    2016-01-01

    The public interest and awareness in healthier lifestyle has increased the demand of functional food, nutraceuticals and designer foods, which has resulted in the creation of new foods in marketplace, for instance designer and specialty eggs. A feeding trial was carried out to evaluate the laying performance of H&N layer chickens fed diets supplemented with tocotrienol rich fraction (TRF), as well as to quantify tocopherol and tocotrienol accumulation in eggs and their effect on egg quality. ...

  18. Effect of Different Levels of L-Carnitine on the Productive Performance, Egg Quality, Blood Parameters and Egg Yolk Cholesterol in Laying Hens

    Directory of Open Access Journals (Sweden)

    Kazemi-Fard M

    2015-12-01

    Full Text Available This experiment was conducted to investigate the effects of different levels of L-carnitine on productive performance, egg quality and blood parameters in laying hens. Forty-eight Hy-Line W-36 egg Layers were weighed at 90 weeks of age and randomly allocated into 16 cages (three hens per cage. Four dietary treatments were prepared by supplementing L-carnitine (0, 50, 100 and 150 mg/kg of diet to corn-soybean meal diet and offered ad libitum to hens. After two weeks of acclimatization, the eggs were weighed daily and feed intake as well as egg quality traits were measured biweekly. At the end of the experiment, two hens from each cage were selected to determine blood parameters and two eggs from each replicate were collected for cholesterol analysis. Results showed that L-carnitine supplementation at 100 and 150 mg/kg significantly increased egg production and egg mass, but decreased yolk cholesterol content. Laying hens receiving diet containing 50 mg/kg L-carnitine had significantly higher Hough unit, but lower progesterone than the hens fed control diet (P < 0.05. The results of this study showed that supplementing hens' diet with L-carnitine had beneficial effects on productive performance and decreased yolk cholesterol concentration; so it can be used as an effective supplement in the diet of laying hens.

  19. Nutritional quality of eggs from hens fed distillers dried grains with solubles.

    Science.gov (United States)

    Trupia, S; Winkler-Moser, J K; Guney, A C; Beckstead, R; Chen, C-Y O

    2016-11-01

    A feeding trial was conducted with laying hens where either 10% or 20% regular-fat distiller's dried grains with solubles (R-DDGS) or low-fat DDGS (L-DDGS) were incorporated into the feed. Production parameters and the effect of DDGS on egg nutritional quality, focusing on yolk lipids, were evaluated. Neither R-DDGS nor L-DDGS at up to 20% of laying hen feeds had a statistically significant impact on hen weight gain, egg production, feed intake, feed efficiency, egg mass, or egg weight. Specific gravity was slightly lower for eggs from hens fed 10% R-DDGS or 20% L-DDGS. Eggs from layers fed DDGS had enhanced levels of tocopherols, tocotrienols, and xanthophylls in the yolk, as well as also increased yolk yellow and red color. Eggs from L-DDGS diet had higher tocopherol content, but eggs from R-DDGS diets had higher xanthophylls. Fatty acid composition in eggs was slightly altered by DDGS, but the ratio of saturated to unsaturated fatty acids was very similar. Feeding DDGS to layer hens had no effect on lecithin or cholesterol content of the eggs. Thus, inclusion of DDGS in the diet of laying hens resulted in increases of several beneficial lipophilic nutrients in egg yolks with no apparent detrimental effects. © 2016 Poultry Science Association Inc.

  20. Egg Speckling Patterns Do Not Advertise Offspring Quality or Influence Male Provisioning in Great Tits

    Science.gov (United States)

    Stoddard, Mary Caswell; Fayet, Annette L.; Kilner, Rebecca M.; Hinde, Camilla A.

    2012-01-01

    Many passerine birds lay white eggs with reddish brown speckles produced by protoporphyrin pigment. However, the function of these spots is contested. Recently, the sexually selected eggshell coloration (SSEC) hypothesis proposed that eggshell color is a sexually selected signal through which a female advertises her quality (and hence the potential quality of her future young) to her male partner, thereby encouraging him to contribute more to breeding attempts. We performed a test of the SSEC hypothesis in a common passerine, the great tit Parus major. We used a double cross-fostering design to determine whether males change their provisioning behavior based on eggshell patterns they observe at the nest. We also tested the assumption that egg patterning reflects female and/or offspring quality. Because birds differ from humans in their color and pattern perception, we used digital photography and models of bird vision to quantify egg patterns objectively. Neither male provisioning nor chick growth was related to the pattern of eggs males observed during incubation. Although heavy females laid paler, less speckled eggs, these eggs did not produce chicks that grew faster. Therefore, we conclude that the SSEC hypothesis is an unlikely explanation for the evolution of egg speckling in great tits. PMID:22815730

  1. Egg speckling patterns do not advertise offspring quality or influence male provisioning in great tits.

    Directory of Open Access Journals (Sweden)

    Mary Caswell Stoddard

    Full Text Available Many passerine birds lay white eggs with reddish brown speckles produced by protoporphyrin pigment. However, the function of these spots is contested. Recently, the sexually selected eggshell coloration (SSEC hypothesis proposed that eggshell color is a sexually selected signal through which a female advertises her quality (and hence the potential quality of her future young to her male partner, thereby encouraging him to contribute more to breeding attempts. We performed a test of the SSEC hypothesis in a common passerine, the great tit Parus major. We used a double cross-fostering design to determine whether males change their provisioning behavior based on eggshell patterns they observe at the nest. We also tested the assumption that egg patterning reflects female and/or offspring quality. Because birds differ from humans in their color and pattern perception, we used digital photography and models of bird vision to quantify egg patterns objectively. Neither male provisioning nor chick growth was related to the pattern of eggs males observed during incubation. Although heavy females laid paler, less speckled eggs, these eggs did not produce chicks that grew faster. Therefore, we conclude that the SSEC hypothesis is an unlikely explanation for the evolution of egg speckling in great tits.

  2. PERFORMANCE AND EGGS QUALITY OF HENS OF GENETIC RESOURCES OF THE CZECH REPUBLIC AND SLOVAKIA

    Directory of Open Access Journals (Sweden)

    Zdeněk Ledvinka

    2015-03-01

    Full Text Available The aim of this study was to assess the performance and technological values of eggs of Czech Hen and Oravka breeds housed on litter. The assumption was that the genotype of hens affects their performance, egg weight, and parameters of yolk, albumen and eggshell quality. A total of 30 pullets of Czech Hen breed and 30 pullets of Oravka breed in the age of 17 weeks were included in to the experiment. Environmental conditions corresponded to the standard requirements for laying hens in observed type of housing system. Hen day egg production, egg yield, daily feed consumption per hen, feed consumption per egg and egg weight, egg shape index, proportion and index of yolk and albumen,yolk colour and Haugh units score were monitored in the experiment. From the parameters of eggshell, proportion, thickness, strength and colour of eggshell were observed. Performance parameters of laying hens weren't affected by the genotype of hens. Czech Hen breed showed insignificantly the higher value in all indicators. The significantly (P≤0.01 and P≤0.001 better values of eggshell quality parameters were detected in Czech Hen breed. We found out that Czech Hen breed had also statistically significantly lighter colour of eggshell. There were no significant interbreed differences in egg weight, proportion of yolk and albumen. The egg shape index was significantly higher in Oravka. Index of yolk and albumen was also significantly (P≤0.001 higher in Oravka. Haugh units score, that reflect the quality of the eggs, were found significantly (P≤0.001higher in Oravka too. On the other hand, yolk colour was detected significantly (P≤0.001 darker for the Czech Hen.

  3. The effects of kale (Brassica oleracea ssp. acephala), basil (Ocimum basilicum) and thyme (Thymus vulgaris) as forage material in organic egg production on egg quality

    DEFF Research Database (Denmark)

    Hammershøj, Marianne; Steenfeldt, Sanna

    2012-01-01

    1. In organic egg production, forage material as part of the diet for laying hens is mandatory. The purpose of the present study was to examine the effect of feeding with forage materials including maize silage, herbs or kale on egg production and various egg quality parameters of the shell, yolk...

  4. Monitoring storage time and quality attribute of egg based on electronic nose

    International Nuclear Information System (INIS)

    Wang Yongwei; Jun Wang; Bo Zhou; Qiujun Lu

    2009-01-01

    The objective of this study was to investigate the potential of an electronic nose (E-nose) technique for monitoring egg storage time and quality attributes. An electronic nose was used to distinguish eggs under cool and room-temperature storage by means of principal component analysis (PCA), linear discriminant analysis (LDA), BP neural network (BPNN) and the combination of a genetic algorithm and BP neural network (GANN). Results showed that the E-nose could distinguish eggs of different storage time under cool and room-temperature storage by LDA, PCA, BPNN and GANN; better prediction values were obtained by GANN than by BPNN. Relationships were established between the E-nose signal and egg quality indices (Haugh unit and yolk factor) by quadratic polynomial step regression (QPSR). The prediction models for Haugh unit and yolk factor indicated a good prediction performance. The Haugh unit model had a standard error of prediction of 3.74 and correlation coefficient 0.91; the yolk factor model had a 0.02 SEP and 0.93 correlation coefficient between predicted and measured values respectively.

  5. The effects of kale (Brassica oleracea ssp. acephala), basil (Ocimum basilicum) and thyme (Thymus vulgaris) as forage material in organic egg production on egg quality.

    Science.gov (United States)

    Hammershøj, M; Steenfeldt, S

    2012-01-01

    1. In organic egg production, forage material as part of the diet for laying hens is mandatory. The purpose of the present study was to examine the effect of feeding with forage materials including maize silage, herbs or kale on egg production and various egg quality parameters of the shell, yolk colour, egg albumen, sensory properties, fatty acid and carotenoid composition of the egg yolk. 2. A total of 5 dietary treatments were tested for 5 weeks, consisting of a basal organic feed plus 120 g/hen.d of the following forage materials: 1) maize silage (control), 2) maize silage incl. 15 g/kg basil, 3) maize silage incl. 30 g/kg basil, 4) maize silage incl. 15 g/kg thyme, or 5) fresh kale leaves. Each was supplied to three replicates of 20 hens. A total of 300 hens was used. 3. Feed intake, forage intake and laying rate did not differ with treatment, but egg weight and egg mass produced increased significantly with the kale treatment. 4. The egg shell strength tended to be higher with the kale treatment, and egg yolk colour was significantly more red with the kale treatment and more yellow with basil and kale treatments. The albumen DM content and albumen gel strength were lowest with the thyme treatment. By sensory evaluation, the kale treatment resulted in eggs with less sulphur aroma, higher yolk colour score, and more sweet and less watery albumen taste. Furthermore, the eggs of the kale treatment had significantly higher lutein and β-carotene content. Also, violaxanthin, an orange xanthophyll, tended to be higher in kale and eggs from hens receiving kale. 5. In conclusion, forage material, especially basil and kale, resulted in increased egg production and eggs of high and differentiable quality.

  6. Chemometric correlation of shelf life, quality measurements, and visible-near infrared spectra of pasteurized eggs

    Science.gov (United States)

    A twelve week study was conducted on the egg albumen from both pasteurized and non-pasteurized shell eggs using visible-near infrared spectroscopy. Correlation of the chemical changes detected in the spectra to the measurement of Haugh units (measure of interior egg quality) was carried out using pr...

  7. Effect of production system and flock age on eggshell and egg internal quality measurements.

    Science.gov (United States)

    Samiullah, Sami; Omar, Amal Saleh; Roberts, Juliet; Chousalkar, Kapil

    2017-01-01

    Egg quality was measured in eggs from different flocks that were reared together and then allocated to different production systems. Eggs were processed for measurements of eggshell and egg internal quality variables, scoring of ultrastructural mammillary layer features, completeness of cuticle cover, and protoporphyrin IX (PP IX) quantification. There was a significant main effect (P egg weight, and egg internal quality and significant effects of flock age on most measurements. The mammillary layer ultrastructural variables showed no clear relationship with production system and flock age. However, there was a significant interaction between production system and flock age for mammillary cap, early and late fusions. Cuticle cover ([Formula: see text]), was significantly higher in barn eggs (19.20), followed by free range (17.57), and cage eggs (15.99). Completeness of cuticle cover was significantly higher in eggs from the 44 week old flock than for 64 week and 73 week old flocks. For eggshells with cuticle intact, there was a significant main effect of both production system and flock age, and significant interaction between the two, for shell reflectivity, L*a*b* values and amount of PP IX. For PP IX, when this difference was calculated for the cuticle alone, there were no statistically significant differences. In 1 g of shell with and without cuticle, there was more PP IX in cage eggs (9.49 × 10 -8 , 7.90 × 10 -8  mM) followed by free range (8.24 × 10 -8 , 6.90 × 10 -8  mM), and barn eggs (8.64 × 10 -8 , 7.28 × 10 -8  mM). Similar trends were recorded for the amount of PP IX in 1 g of cuticle, but the difference was not statistically significant. The amount of PP IX decreased significantly with increasing flock age. Comparing the cage and barn production systems at 68 week of flock age, there was no difference for the amount of PP IX in shell with or without cuticle, or in the cuticle alone. Eggs from the cage production system were darker in color

  8. Microbial quality of industrial liquid egg white: assumptions on spoiling issues in egg-based chilled desserts.

    Science.gov (United States)

    Techer, Clarisse; Daoud, Amina; Madec, Marie-Noëlle; Gautier, Michel; Jan, Sophie; Baron, Florence

    2015-02-01

    As a 1st step, this study aimed at investigating the microbial quality of liquid egg white in a French egg processing company. Thirty raw and 33 pasteurized liquid egg white samples were analyzed. Pasteurization was globally found efficient on mesophilic contaminants (1.7 ± 1.6 and 0.8 ± 0.9 log CFU/mL in raw and pasteurized samples, respectively), including for the control of Salmonella. However, Gram-positive enterococci were still detected in the pasteurized samples. As a 2nd step, a representative bacterial collection was built for exploring the spoilage issue in egg-based chilled desserts. Custard cream was chosen as growth medium since this food is widely used for the production of French chilled desserts. All of the 166 isolates of the bacterial collection were shown to be able to grow and to induce spoilage of the custard cream at refrigeration temperature (10 °C). Several spoilage types were highlighted in the custard cream, on the basis of changes regarding pH, consistency, production of holes or gas. As a 3rd step, bacterial enzymatic activities were explored on custard cream-based agar media. The bacterial collection was reduced to 43 isolates, based on further selection regarding the genera and the spoilage types previously highlighted. Albeit to different degrees, all these isolates were able to produce proteases. A large part of these isolates also expressed lipolytic and amylolytic activities. This study emphasizes the need to control egg white contamination and especially with Gram-positive heat-resistant Enterococi, in order to guarantee the shelf life of egg-based chilled desserts. © 2015 Institute of Food Technologists®

  9. Effect of Egg Orientation on Malposition in Hatching Eggs

    Directory of Open Access Journals (Sweden)

    İsmail Durmuş

    2015-09-01

    Full Text Available This research was conducted to determine the effects of orientation in the hatching eggs on hatching results and position disorders, so the eggs were placed in incubator for 3 groups as orientation of normal, horizontal and small end up. In this study, 1680 hatching eggs were divided into 3 groups with 7 replicates of 80 eggs in each. Hatchability of fertile eggs, malformation ratio, malposition ratio, weight loss ratio, chick quality, embryo death ratios in the early, middle and late stage of embriogenesis and mortality ratio were investigated in this research. There were significant differences among the groups in terms of egg weight loss ratio, embryo death in the late stage of embriogenesis, malposition ratio, hatching rate , whereas no significant differences among the groups in respect to malformation ratio, chick quality, embryo death in early and late embriogenesis. It was determined that the late embrionic mortality increased because of the position errors in case of hatching eggs orientation and the head of the embryo in small end up of eggs.

  10. Bioresonance method influencing the egg quality

    International Nuclear Information System (INIS)

    Avakova, A.; Kovalev, Yu.

    2009-01-01

    At 2 poultry yards with capacity of 30 thousand laying hens an experiment was undertaken to study the bioresonance effect on 18-week-old poultry (experimental poultry yard, EPY) to obtain eggs of high biological value by activating the assimilation by poultry of a microelement complex from food. At the EPY hens received a vitamin-mineral complex Lifepac senior (produced in France) treated with electromagnetic radiation. At the control poultry yard (CPY) this method was not used. The first egg collection was conducted from hens at the age of 28 weeks, the second one – at the age of 3-4 weeks and the third one – at the age of 52 weeks. The keeping and feeding conditions at the two poultry yards were the same. The contents of water, protein, fat in the eggs of CPY and EPY were practically the same, but the level of microelements in the EPY eggs was higher than in CPY eggs. It was observed so as to maintain high level of microelements in eggs it is required to a bioresonance effect throughout the egg laying period. So, the level of manganese in eggs of EPY and CPY at week 52 was 0.024 and 0.003 mg%, iron – 3.74 and 1.48 mg%, zinc – 1.23 and 0.87 mf%, copper – 0.078 and 0.069 mg%, respectively. The transition of microelements from food into eggs also was % to the control: iron – 2.55, manganese – 10.0, zinc – 1.44, copper – 1.35, potassium – 1.33, calcium – 1.05, phosphorus – 1.15, sodium – 1.16. Thus, the bioresonance method for keeping laying hens makes it possible to obtain food eggs of the desired chemical composition

  11. Effect of distance from the roadway on heavy metal content and egg quality of village laying hen's egg along roadsides of tokat-turhal, turkey

    International Nuclear Information System (INIS)

    Sekeroglu, A.; Sari, H.; Sarica, M.; Duman, M.

    2013-01-01

    In this study, the heavy metal contents and egg quality characteristics of chicken eggs was studied. The eggs have been collected at distances of 0-100 (Distance A), 100-200 (Distance B) and 200-300 m (Distance C) away from the edge of Tokat-Turhal road.The distance from roadway had no significant effect on egg Cu and Mn contents (P>0.05), although a significant effect on egg Cd and Pb content (P 0.05). Eggs obtained from distance A and B had significantly higher weight than obtained from distance C (P<0.05). Egg obtained from distance B had significantly lower egg shell colour scales than those of the other distances (P<0.01). And, egg yolk colour was showed a significant variation among the distances away from the roadway, and the highest yolk colour value was detected in distance B (P<0.05). Depending on obtained results, it can be concluded that the egg heavy metal concentrations exceeded background levels for hen eggs. Therefore, consumers should be aware of the possible health risks related by the use of these eggs which has obtained from side of road.

  12. Pasta quality as impacted by the type of flour and starch and the level of egg addition.

    Science.gov (United States)

    Saleh, Mohammed; Al-Ismail, Khalid; Ajo, Radwan

    2017-10-01

    This study investigated the effects of substituting wheat flour with fractions of different starch types and egg levels on pasta quality. First order mixture response surface model was used where the effects of various starch types and egg levels on pasta quality were evaluated. Coefficients of estimation were determined and fractional contribution of wheat, starch type and egg levels were evaluated. Egg levels negatively (p egg level from 33 to 0%. Flow behavior index of treatments solution with various fractions of starch types and egg level ranged from 0.34 to 1.42 and was significantly (p cooked pasta quality including firmness, stickiness, cooking loss, and water uptake, keeping with consumer acceptability through varying starch type and egg level. Results show that flour and starch type and egg level interaction play significant role in pasta blends formulation. Moreover, substitution of wheat flour with acorn, native or modified corn and potato starches fractions, as well as with lupine, rice, tapioca, and stabilized rice bran flours would have significant effects on the physical properties and acceptability of various cereal products. For instance, the use of rice bran in potentially developed products would enhance the consumption of whole grain foods, resulting in improved intake of fiber and other healthy components. © 2016 Wiley Periodicals, Inc.

  13. Effect of cadmium doses on chickens. 3. Long term influence of cadmium on feed consumption weight gain, egg performance and egg shell quality of laying hens

    Energy Technology Data Exchange (ETDEWEB)

    Suelz, M; Hardebeck, H; Krampitz, G

    1974-01-01

    In long-lasting experiments the application of Cd resulted in a decreased state of health (nephritis) of hens. Feed consumption, weight gain and egg production were reduced. Egg shell quality was not changed under practical Cd-concentrations. Studies of the ultrastructure of egg shells of animals fed with Cd did not yield any hints of damages. The protein-profiles of egg shells revealed an additional component under Cd-application. 17 references, 2 figures, 2 tables.

  14. Egg quality traits and predictors of embryo and fry viability in red snapper Lutjanus campechanus

    Directory of Open Access Journals (Sweden)

    Agnès Bardon-Albaret

    2017-08-01

    Full Text Available The quality of red snapper eggs is highly variable and unpredictable in aquaculture, leading to high mortality during early larval rearing. In this work, the viability of red snapper eggs was monitored from fertilization until unfed larvae expired because of exhaustion of vitelline reserves to determine egg quality traits in this species. The spawns were obtained via strip spawning wild-caught females following hormonal induction with chorionic gonadotropin. Females were induced immediately after capture (wild group, n = 17 or held captive for the entire maturation period prior to induction (captive group, n = 7. Candidate predictors of egg quality measured on the female parent at the time of induction or on the spawn at ovulation were evaluated using correlation and multiple regression analysis. The fertilization rate, the hatching rate, and the duration of survival of unfed larvae post hatch were weakly correlated to each other (-0.23 < r < −0.08, revealing occurrence of distinct and independent components of egg quality. Spawns from captive females were characterized by a longer latency interval between hormonal induction and ovulation, lower fecundity, and lower hatching rates, as compared to those from wild females. Among the wild brood fish, a positive correlation was observed between the age of the female and the hatching rate. The best model optimized during stepwise multiple regression analysis of hatching rate data only explained 34% of the variance for this trait and no model could be optimized for the prediction of fertilization rate or the duration of survival post hatch. These results highlight the need to develop alternative egg quality measures to predict the viability of fry with confidence.

  15. Effects of egg storage on hatchability, chick quality, performance and immunocompetence parameters of broiler chickens.

    Science.gov (United States)

    Goliomytis, Michael; Tsipouzian, Theofania; Hager-Theodorides, Ariadne L

    2015-09-01

    Pre-incubation egg storage is a necessity for the poultry industry. This study evaluated the effects of pre-incubation storage length of broiler eggs on hatchability, 1-day-old chick quality, subsequent performance, and immunocompetence. To this end, a total of 360 hatching eggs were stored for 4, 12, or 16 d prior to incubation. Hatchability and chick quality were assessed at hatch, and growth performance and immunocompetence parameters were assessed during a 35 d rearing period. Hatchability of set and fertile eggs, and embryonic mortality, were not affected by egg storage. On the contrary, 1-day-old chick BW and length were linearly negatively correlated with egg storage length (P-linearegg weight prior to setting was unaffected, and corrected chick length was positively affected by storage length. One-day-old chick Tona score, navel quality, and post-hatch growth performance (BW at 7 and 35 d, cumulative feed intake, and feed conversion ratio at 35 d) were unaffected by egg storage (P, P-linear>0.05). Lymphoid organ weights at 2 and 35 d, the titre of maternal anti-NDV antibodies, most of the thymocyte subpopulations defined by CD3, CD4, and CD8 cell surface expression in the thymus of 2-d-old chicks, cellular responses to the PHA skin test, humoral responses to primary SRBC, and NDV immunizations were also not influenced by length of storage (P, P-linear>0.05). On the contrary, the length of egg storage was found to negatively influence the abundance of CD3+CD4-CD8- thymocytes that represent the majority of γδ-T cells in the thymus of 2-day-old chicks, as well as the humoral response to booster NDV immunization of the birds. In brief, pre-incubation storage of broiler hatching eggs for up to 16 d did not affect most developmental and growth parameters investigated, except for BW and length at hatch. Egg storage was found to suppress some aspects of the immunocompetence of the birds, particularly aspects of acquired immunity. © 2015 Poultry Science

  16. Influence of Zeolite on fatty acid composition and egg quality in Tunisian Laying Hens

    Science.gov (United States)

    2012-01-01

    Background The health benefits of omega-3 and omega-6 polyunsaturated fatty acids (PUFA) are generally recognized. Unfortunately, in most Mediterranean countries, the recommended daily intake of these compounds is rarely met. Therefore, enrichment of commonly occurring foods can boost intake of these fatty acids. In this regard, eggs are an interesting target, as they form an integral part of the diet. Result Zeolite (Clinoptilolites) was added to Laying Hens feed at concentrations 1% or 2% and was evaluated for its effects on performance of the production and on egg quality. The Laying Hens were given access to 110 g of feed mixtures daily that was either a basal diet or a ‘zeolite diet’ (the basal diet supplemented with clinoptilolite at a level of 1% or 2%). It was found that zeolite treatment had a positive and significatif (p zeolite supplementation tended to/or has no significant effects on total egg, eggshell, yolk and albumen weights. It was found also that zeolite mainly increases level of polyunsaturated fatty acids in egg. Conclusion This study showed the significance of using zeolite, as a feed additive for Laying Hens, as part of a comprehensive program to control egg quality and to increase level of polyunsaturated fatty acids on egg. PMID:22676421

  17. Hen genetic strain and extended cold storage influence on physical egg quality from cage-free aviary housing system.

    Science.gov (United States)

    Jones, D R; Karcher, D M; Regmi, P; Robison, C O; Gast, R K

    2018-04-02

    In the United States, there is an increase in need for cage-free eggs in retail and food manufacturing sectors. Understanding the impact of cage-free systems and the corresponding management on egg quality is pertinent as the U.S. industry adapts existing housing and builds new cage-free housing structures. A study was conducted comparing 2 brown shell and 2 white shell hen strains housed in a cage-free aviary system. Each set of eggs were placed in cold storage and assessed at 0, 2, 4, 8, and 12 wk. Eggs were collected at 21, 31, 42, and 60 wk of hen age. A full profile of physical quality measurements was conducted on up to 18 intact eggs for each hen strain/egg storage/hen age combination. Egg weight increased approximately 10 g for brown shell and 14 g for white shell eggs as hens aged. Many of the properties monitored were significantly impacted by all 3 main effects (hen strain, egg storage, and hen age) resulting in 3-way interactions. A brown and a white shell strain had stronger shells (44 N; P study also determined volume of shell, total length, maximum width, and percent length at maximum width to more accurately indicate egg shape than shape index. One brown shell strain produced eggs with the most consistent shape characteristics over the hen ages monitored. White shell eggs from the cage-free aviary housing produced the highest whole-egg total solids between 31 to 60 wk of hen age, whereas brown shell eggs resulted in the most consistent level of whole-egg total solids (22-23.5%). The brown and white shell strains in the current study produce cage-free aviary eggs with distinctive physical quality attributes. The outcomes from this study can be utilized by the U.S. egg industry in planning management strategies and market placement of cage-free eggs.

  18. Effects of preincubation of eggs and activation medium on the percentage of eyed embryos in ide (Leuciscus idus), an externally fertilizing fish.

    Science.gov (United States)

    Siddique, Mohammad Abdul Momin; Linhart, Otomar; Krejszeff, Sławomir; Żarski, Daniel; Król, Jarosław; Butts, Ian Anthony Ernest

    2016-03-15

    Standardization of fertilization protocols is crucial for improving reproductive techniques for externally fertilizing fish in captive breeding. Therefore, the objectives of this study were to determine the effects of preincubation of eggs and activation medium on the percentage of eyed embryos for ide (Leuciscus idus). Pooled eggs from five females were preincubated in three different activating media for 0, 30, 60, 90, and 120 seconds and then fertilized by pooled sperm from five males. At the eyed-egg stage, the percentage of viable embryos was later calculated. Results showed that preincubation time was significant for the freshwater activation medium (P reproduction for this species. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. Efficacy of antimicrobial pullulan-based coating to improve internal quality and shelf-life of chicken eggs during storage.

    Science.gov (United States)

    Morsy, Mohamed K; Sharoba, Ashraf M; Khalaf, Hassan H; El-Tanahy, Hassan H; Cutter, Catherine N

    2015-05-01

    There has been a growing interest in the use of natural materials as a delivery mechanism for antimicrobials and coatings in foods. The aim of the present study was to evaluate the effectiveness of pullulan coatings to improve internal quality and shelf-life of fresh eggs during 10 wk of storage at 25 and 4 °C. Three treatments of eggs were evaluated as follows; non-coated (control; C), coated with pullulan (P), and coated with pullulan containing nisin (N). The effects of the pullulan coatings on microbiological qualities, physical properties, and freshness parameters were investigated and compared with non-coated eggs. For non-coated eggs, as storage time increased, yolk index, albumen index, and Haugh unit value decreased and weight loss increased. However, pullulan coatings (P or N) minimized weight loss (eggs (with a final B grade) 3 wk longer than non-coated eggs at 25 °C. At 4 °C, both P- and N-coated eggs went from AA to A grade after 9 wk and maintained the grade for 10 wk (4 wk longer than that of non-coated eggs). This study is the first to demonstrate that pullulan coatings can preserve the internal quality, prolong the shelf-life, and minimize weight loss of fresh eggs. © 2015 Institute of Food Technologists®

  20. Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen.

    Science.gov (United States)

    Chanapiwat, Panida; Kaeoket, Kampon; Tummaruk, Padet

    2009-09-01

    The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk (group I), DHA-enriched hen egg yolk (group II), normal hen egg yolk with 5 mmol L(-1) of cysteine supplementation (group III) and DHA-enriched hen egg yolk with 5 mmol L(-1) of cysteine supplementation (group IV). The semen was cryopreserved using controlled rate freezer and was thawed at 50 degrees C for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone (group III) improved progressive motility (P semen qualities (P > 0.05). In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.

  1. Differentiating sex and species of Western Grebes (Aechmophorus occidentalis) and Clark's Grebes (Aechmophorus clarkii) and their eggs using external morphometrics and discriminant function analysis

    Science.gov (United States)

    Hartman, C. Alex; Ackerman, Joshua T.; Eagles-Smith, Collin A.; Herzog, Mark

    2016-01-01

    In birds where males and females are similar in size and plumage, sex determination by alternative means is necessary. Discriminant function analysis based on external morphometrics was used to distinguish males from females in two closely related species: Western Grebe (Aechmophorus occidentalis) and Clark's Grebe (A. clarkii). Additionally, discriminant function analysis was used to evaluate morphometric divergence between Western and Clark's grebe adults and eggs. Aechmophorus grebe adults (n = 576) and eggs (n = 130) were sampled across 29 lakes and reservoirs throughout California, USA, and adult sex was determined using molecular analysis. Both Western and Clark's grebes exhibited considerable sexual size dimorphism. Males averaged 6–26% larger than females among seven morphological measurements, with the greatest sexual size dimorphism occurring for bill morphometrics. Discriminant functions based on bill length, bill depth, and short tarsus length correctly assigned sex to 98% of Western Grebes, and a function based on bill length and bill depth correctly assigned sex to 99% of Clark's Grebes. Further, a simplified discriminant function based only on bill depth correctly assigned sex to 96% of Western Grebes and 98% of Clark's Grebes. In contrast, external morphometrics were not suitable for differentiating between Western and Clark's grebe adults or their eggs, with correct classification rates of discriminant functions of only 60%, 63%, and 61% for adult males, adult females, and eggs, respectively. Our results indicate little divergence in external morphology between species of Aechmophorus grebes, and instead separation is much greater between males and females.

  2. Ovary transcriptome profiling via artificial intelligence reveals a transcriptomic fingerprint predicting egg quality in striped bass, Morone saxatilis.

    Directory of Open Access Journals (Sweden)

    Robert W Chapman

    Full Text Available Inherited gene transcripts deposited in oocytes direct early embryonic development in all vertebrates, but transcript profiles indicative of embryo developmental competence have not previously been identified. We employed artificial intelligence to model profiles of maternal ovary gene expression and their relationship to egg quality, evaluated as production of viable mid-blastula stage embryos, in the striped bass (Morone saxatilis, a farmed species with serious egg quality problems. In models developed using artificial neural networks (ANNs and supervised machine learning, collective changes in the expression of a limited suite of genes (233 representing 90% of the eventual variance in embryo survival. Egg quality related to minor changes in gene expression (<0.2-fold, with most individual transcripts making a small contribution (<1% to the overall prediction of egg quality. These findings indicate that the predictive power of the transcriptome as regards egg quality resides not in levels of individual genes, but rather in the collective, coordinated expression of a suite of transcripts constituting a transcriptomic "fingerprint". Correlation analyses of the corresponding candidate genes indicated that dysfunction of the ubiquitin-26S proteasome, COP9 signalosome, and subsequent control of the cell cycle engenders embryonic developmental incompetence. The affected gene networks are centrally involved in regulation of early development in all vertebrates, including humans. By assessing collective levels of the relevant ovarian transcripts via ANNs we were able, for the first time in any vertebrate, to accurately predict the subsequent embryo developmental potential of eggs from individual females. Our results show that the transcriptomic fingerprint evidencing developmental dysfunction is highly predictive of, and therefore likely to regulate, egg quality, a biologically complex trait crucial to reproductive fitness.

  3. Slow fertilization of stickleback eggs: the result of sexual conflict?

    Directory of Open Access Journals (Sweden)

    Frommen Joachim G

    2006-05-01

    Full Text Available Abstract Background The fertilization success in sperm competition in externally fertilizing fish depends on number and quality of sperm. The time delay between sequential ejaculations may further influence the outcome of sperm competition. Such a time interval can load the raffle over fertilization if fertilization takes place very fast. Short fertilization times are generally assumed for externally fertilizing fish such as the three-spined stickleback (Gasterosteus aculeatus. In this pair-spawning fish, territorial males often try to steal fertilizations in nests of neighbouring males. This sneaking behaviour causes sperm competition. Sneakers will only get a share of paternity when eggs are not fertilized immediately after sperm release. Contrary to males, females may be interested in multiple paternity of their clutch of eggs. There thus may be a sexual conflict over the speed of fertilization. Results In this study we used two different in vitro fertilization experiments to assess how fast eggs are fertilized in sticklebacks. We show that complete fertilization takes more than 5 min which is atypically long for externally fertilizing fishes. Conclusion This result suggests that the time difference does not imply high costs to the second stickleback male to ejaculate. Slow fertilization (and concomitant prolonged longevity of sperm may be the result of sexual conflict in which females aimed at complete fertilization and/or multiple paternity.

  4. Performance, egg quality, and liver lipid reserves of free-range laying hens naturally infected with Ascaridia galli.

    Science.gov (United States)

    Sharma, N; Hunt, P W; Hine, B C; Sharma, N K; Chung, A; Swick, R A; Ruhnke, I

    2018-03-16

    A study was conducted to determine the performance, egg quality, and liver lipid reserves of laying hens exposed to ranges contaminated with Ascaridia galli. Sixteen-week-old Lohmann Brown laying hens (n = 200) were divided into 4 treatments with 5 replicates containing 10 hens per pen. Hens of treatment 1 [negative control (NC)] ranged on a decontaminated area, and hens of treatments 2 (low infection) and 3 (medium infection) ranged on areas previously contaminated by hens artificially infected with 250 and 1,000 embryonated A. galli eggs, respectively. The hens of treatment 4 [positive control (PC)] ranged on areas previously contaminated by hens artificially infected with 2,500 embryonated A. galli eggs, and in addition these hens were orally inoculated with 1,000 embryonated eggs. Results indicated that hens of the medium infection group had a higher number of intestinal A. galli worms and A. galli eggs in the coprodeum excreta (43.9 ± 4.0 and 3,437 ± 459 eggs/g) compared to hens of the low infection group (23.8 ± 4.0 and 1,820 ± 450 eggs/g) (P  0.05). Egg production, egg mass, feed intake, and feed conversion ratio (FCR) were not affected by A. galli infection (P > 0.05). Egg quality parameters (egg weight, shell reflectivity, shell weight, shell thickness, shell percentage, shell breaking strength, deformation, albumen height, Haugh unit, and yolk score) were not affected by A. galli infection (P > 0.05). Highly infected hens had lower liver lipid content (2.72 ± 0.51 g) compared to uninfected hens (4.46 ± 0.58 g, P ranges contaminated with A. galli resulted in infection of the ranging hens, but this did not affect egg production or egg quality. Infection with A. galli lowered the liver lipid reserves of the host significantly, suggesting infected hens use more energy reserves for maintenance and production.

  5. Exterior and interior physical quality of egg of laying hens fed diets containing different dietary purslane levels

    Science.gov (United States)

    Kartikasari, L. R.; Hertanto, B. S.; Pranoto, D.; Salim, W. N.; Nuhriawangsa, A. M. P.

    2017-04-01

    Purslane is considered a rich vegetable source of alpha-linolenic acid, beta-carotene and various antioxidants. The objective of the study was to investigate the effect of different dietary levels of purslane meal (Portulaca oleracea) in the diets of laying hens on physical quality of eggs. A total of 125 Hy-Line Brown hens (54 weeks old) were placed at individual cages and assigned to five dietary treatments. The diets were supplemented with 0, 2, 4, 6 and 8% purslane meal. Laying hens were fed for 5 weeks after a typical period of adaptation (7 days). Water and feed were provided ad libitum. A total of 25 egg samples of day 28 and day 35 (n = 5 egg yolks for each treatment) were collected to analyse exterior and interior physical quality of eggs. The data were analysed using ANOVA. Differences between treatment means were further analysed using Duncan’s New Multiple Range Test. Results showed that feeding different purslane meal levels in the diets improved egg weight, yolk weight, albumen weight and yolk colour. The highest intensity of yolk colour was obtained with the diet containing 8% purslane meal. However, dietary treatments did not affect egg index, albumen index, yolk index, shell weight, shell thickness and Haugh Unit. It is concluded that including purslane meal to laying hen diets increases the physical qualities of the eggs.

  6. Egg quality parameters and blood biochemical profile of six strains ...

    African Journals Online (AJOL)

    Six different poultry strains (Indigenous chicken, Broiler, Turkey, Geese, Duck and Guinea fowl) were studied under extensive system of management to investigate the effect of rearing system on their egg quality and the blood biochemical profile, respectively. Birds used for the study were obtained from four different ...

  7. Influence of Zeolite on fatty acid composition and egg quality in Tunisian Laying Hens

    Directory of Open Access Journals (Sweden)

    Fendri Imen

    2012-06-01

    Full Text Available Abstract Background The health benefits of omega-3 and omega-6 polyunsaturated fatty acids (PUFA are generally recognized. Unfortunately, in most Mediterranean countries, the recommended daily intake of these compounds is rarely met. Therefore, enrichment of commonly occurring foods can boost intake of these fatty acids. In this regard, eggs are an interesting target, as they form an integral part of the diet. Result Zeolite (Clinoptilolites was added to Laying Hens feed at concentrations 1% or 2% and was evaluated for its effects on performance of the production and on egg quality. The Laying Hens were given access to 110 g of feed mixtures daily that was either a basal diet or a ‘zeolite diet’ (the basal diet supplemented with clinoptilolite at a level of 1% or 2%. It was found that zeolite treatment had a positive and significatif (p  Conclusion This study showed the significance of using zeolite, as a feed additive for Laying Hens, as part of a comprehensive program to control egg quality and to increase level of polyunsaturated fatty acids on egg.

  8. Low-fiber alfalfa (Medicago sativa L.) meal in the laying hen diet: effects on productive traits and egg quality.

    Science.gov (United States)

    Laudadio, V; Ceci, E; Lastella, N M B; Introna, M; Tufarelli, V

    2014-07-01

    This study was designed to determine the effects on laying performance and egg quality resulting from partial substitution of soybean meal (SBM) with low-fiber alfalfa (LFA; Medicago sativa L.) meal in the diet of early-phase laying hens. ISA Brown layers, 18 wk of age, were randomly allocated to 2 dietary treatments and fed for 10 wk. The hens were fed 2 wheat middling-based diets: a control diet, which contained SBM (15% of diet), and a test diet containing LFA (15% of diet) as the main protein source. Low-fiber alfalfa meal was obtained by a combination of sieving and air-classification processes. Feed intake was recorded daily, and egg production was calculated on a hen-day basis; eggs from each group were weekly collected to evaluate egg components and quality. The partial substitution of SBM with LFA had no adverse effect on growth performance of early-phase laying hens. Egg production and none of the egg-quality traits examined were influenced by dietary treatment, except for yolk color (P alfalfa meal in the laying-hen diet can positively influence yolk quality without adversely affecting productive traits. © 2014 Poultry Science Association Inc.

  9. Producing and marketing a specialty egg.

    Science.gov (United States)

    Michella, S M; Slaugh, B T

    2000-07-01

    Eggland's Best, Inc. markets premium quality shell eggs under the Eggland's Best (EB) brand name. The company, started in 1988, is comprised of a franchise network of established egg producers and covers most of the United States. Eggland's Best provides its franchisees with marketing and technical support. The franchisees produce, process, and distribute EB eggs according to the strict program established and monitored by EB. Production follows the all-natural vegetarian feed program in accordance with the company's US patent entitled "Eggs Compatible with a Cholesterol Reducing Diet and Method of Producing the Same." The EB program excludes animal fat and other animal byproducts. Eggland's Best eggs have seven times the generic level of vitamin E, nearly three times more omega-3 fatty acids and iodine, and 25% less saturated fat than regular generic eggs. Eggland's Best has one of the finest shell egg quality assurance programs anywhere. Eggland's Best franchisees submit weekly egg samples that are analyzed for shell quality, interior quality, vitamin E, iodine, cholesterol and fatty acids. Samples of feed and the EB-patented feed supplement are also analyzed. Approximately 28,000 total laboratory tests are conducted annually. Nationwide product and display retail evaluations are contracted through an outside audit company (40 to 50 cities evaluated four times per year). All EB eggs are USDA graded according to EB's strict quality standards. Producers must follow a food safety quality assurance program (United Egg Producers Association 5-Star or equivalent state or company program). Each egg is stamped "EB" as assurance of meeting EB's highest standards of flavor, quality, and nutrition. Eggland's Best has enjoyed record sales growth for the past 3 yr.

  10. Performance response and egg qualities of laying birds fed enzyme ...

    African Journals Online (AJOL)

    Theperformance response and egg qualities o laying birds fed enzyme supplemented PKC diets asreplacement for maize was investigated wth 210, 20 week old layng pullets of Dominant Black strain at the Teaching and Research Farm of the Delta State University, Asaba Campus, Nigeria. The birds which ust come into ...

  11. Effect of Different Levels of Surplus Date on Performance, Egg Quality and Blood Parameters in Laying Hens

    Directory of Open Access Journals (Sweden)

    S.J Hosseini Vashan

    2011-12-01

    Full Text Available One hundred forty-four 26-wk-old white Hy-Line layers (W-36 were fed commercial diets containing 0, 1.5, 3 and 5% surplus date for three period of 28 days each to study the effects of dietary surplus date on hen performance (egg production, egg weight, egg mass, feed intake, feed conversion ratio ‘FCR’ and body weight gain and egg quality parameters (Haugh unit score, yolk colour index, yolk index, egg shape, shell weight, shell thickness and density. The yolk of eggs extracted and cholesterol content were determined on one egg of each replicate hens in each period. Blood samples were collected in non-heparin zed tubes from six hens in each treatment through brachial vein at the end of experiment. Serum was separated after 8-10 hrs and was stored at – 20 oC for subsequent analysis. Hen performance (egg production, egg mass, feed intake, FCR and weight gain and egg quality parameters (Haugh unit score, yolk colour index, yolk index, egg shape, shell weight, shell thickness and density were not significantly different among treatments (P>0.05; However in all traits, the control group had numerically lower value, except egg weight that was significantly increased with supplementation of surplus date in diet. The dietary surplus date did not significantly affect egg cholesterol, ND and IBD titre, but the serum cholesterol was significantly reduced in hens fed diets contained 5% surplus date. This study suggested that the surplus date may be used up to 5% in the diet of laying hens to reduce blood cholesterol without any significant adverse effect on performance.

  12. Inactivation of Salmonella in Shell Eggs by Hot Water Immersion and Its Effect on Quality.

    Science.gov (United States)

    Geveke, David J; Gurtler, Joshua B; Jones, Deana R; Bigley, Andrew B W

    2016-03-01

    Thermal inactivation kinetics of heat resistant strains of Salmonella Enteritidis in shell eggs processed by hot water immersion were determined and the effects of the processing on egg quality were evaluated. Shell eggs were inoculated with a composite of heat resistant Salmonella Enteritidis (SE) strains PT8 C405, 2 (FSIS #OB030832), and 6 (FSIS #OB040159). Eggs were immersed in a circulating hot water bath for various times and temperatures. Come-up time of the coldest location within the egg was 21 min. SE was reduced by 4.5 log at both hot water immersion treatments of 56.7 C for 60 min and 55.6 °C for 100 min. Decimal reduction times (D-values) at 54.4, 55.6, and 56.7 °C were 51.8, 14.6, and 9.33 min, respectively. The z-value was 3.07 °C. Following treatments that resulted in a 4.5 log reduction (56.7 °C/60 min and 55.6 °C/100 min), the surviving population of SE remained static during 4 wk of refrigerated storage. After processing under conditions resulting in 4.5 log reductions, the Haugh unit and albumen height significantly increased (P eggs by 4.5 log, but also significantly affected several egg quality characteristics. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.

  13. Effects of dietary clinoptilolite and calcium levels on the performance and egg quality of commercial layers

    Directory of Open Access Journals (Sweden)

    DA Berto

    2013-09-01

    Full Text Available Among the different feed additives studied in poultry production, clinoptilolite, an aluminosilicate capable of adsorbing harmful substances and of improving live performance and egg and meat quality, was evaluated. The objective of the present study was to evaluate the influence of dietary clinoptilolite and calcium levels on the performance and egg quality of layers. In total, 576 layers were distributed according to a completely randomized experimental design in a 3 x 4 factorial arrangement (three calcium levels - 2.5, 3.1, or 3.7% and four clinoptilolite levels - 0.0, 0.15, 0.25, or 0.50%, with 12 treatments of six replicates of eight birds each. The experiment included four 28-d cycles. The experimental diets were based on corn and soybean meal. Results were submitted to analysis of variance and means were compared by the test of Tukey at 5% significance level using SISVAR statistical package. There was a significant interaction between the evaluated factors for egg production and feed conversion ratio per dozen eggs and egg mass. The lowest calcium level resulted in worse performance and eggshell quality. Clinoptilolite levels affected albumen and yolk content. It was concluded that up to 0.50% inclusion of clinoptilolite in layer diets does not benefit layer performance or eggshell quality. Although the inclusion of only 2.5% calcium in layer diets is not recommended, it is possible to add 3.1% because it promoted similar results as the recommended level of 3.7%.

  14. Comparison of two different breeding systems laying hens in relation to egg shell quality, II

    Directory of Open Access Journals (Sweden)

    Mária Angelovičová

    2014-11-01

    Full Text Available The aim of work was to follow up and statistically evaluate the selected quality indicators of egg shell according to two different breeding systems and different age of laying hens. An object of investigation were shell weight, share of the shell, strength and thickness of the shell for table eggs. There were used the laying hens of final hybrid ISA Brown reared in enriched cage system, and free range system. In both breeding systems were ensured the conditions with application of the welfare principles. There was used to feed a complete feed mixture HYD 10 in the both breeding systems.  The feeders were supplemented with feed by hand, daily and the same day was supplemented water to drinking troughs. Egg collection was hand in both breeding systems. This paper is a contribution to the solution of optimal breeding laying hens and production of high quality and safe production of table eggs. From the evaluation of the results was formulated conclusion, which shows that statistically significant (p ≤ 0.05 higher egg shell thickness was observed in the breeding free range system compared to the thickness of the egg shell in the breeding cage system, and in age 40 weeks of laying hens in both breeding systems compared to the thickness of the egg shell in age 30 weeks of laying hens. No statistically significant difference (p ≥ 0.05 was observed in egg shell weight between breeding cage system and free range system. Statistically significant (p ≤ 0.05 higher egg shell weight was observed in the age 40 weeks of laying hens in both breeding  systems compared to age 30 weeks of laying hens. There no statistically significant difference (p ≥ 0.05 was observed in the share of egg shell and egg shell strength between breeding cage system and free range system, nor between age 30 and 40 weeks of laying hens.

  15. Assessing Consumer Emotional Responses in the Presence and Absence of Critical Quality Attributes: A Case Study with Chicken Eggs.

    Science.gov (United States)

    Wardy, Wisdom; Sae-Eaw, Amporn; Sriwattana, Sujinda; No, Hong Kyoon; Prinyawiwatkul, Witoon

    2015-07-01

    Effects of attribute presence and absence on the emotional profile and consumer acceptability of products with varying qualities were assessed using eggs as an example. An online survey (n = 320) was used to evaluate emotional responses and acceptability to 5 types of egg quality attributes: intrinsic, aesthetic, extrinsic, expediency, and wholesome/safety, for both present and absent conditions. Attribute absence rather than presence evoked greater consumer discriminating emotions associated with eggs. Mean emotion intensity elicited by the presence of all quality attributes ranged from 1.67 (intrinsic; guilty) to 4.05 (wholesome; good) versus 2.01 (wholesome; satisfied) to 3.29 (wholesome; disgusted) when absent. Key positive emotions elicited by presence of attributes were active, calm, good, interested, happy, safe, and satisfied; while dominant negative emotions elicited by absence of attributes included disgusted and worried. Wholesome quality (constituted by egg freshness, "packing/best-before-date" and absence of visible cracks) exhibited the highest liking (7.65) and emotion intensities, while the emotional responses to both the presence and absence of intrinsic quality (constituted by nutrient-fortified egg, organic egg, and USDA-certified farm egg) were similar, reflecting their dynamic effects on emotions. Emotions and acceptability were more correlated for attribute absence than presence; and good, happy, and satisfied emotions were strongly related to egg acceptability (r ≥ 0.6). Egg product/packaging design can be oriented toward emphasizing wholesome and expedient attributes, since they enhance good, safe, and satisfied emotions, while minimizing disgust, worry, and boredom. The use of emotional responses and hedonic testing regarding attribute presence and absence would allow for improved selection of attributes critical to consumer acceptance of products. Assessing effects of attribute presence compared with absence on food-evoked emotions may

  16. Effects of energy concentration of the diet on productive performance and egg quality of brown egg-laying hens differing in initial body weight.

    Science.gov (United States)

    Pérez-Bonilla, A; Novoa, S; García, J; Mohiti-Asli, M; Frikha, M; Mateos, G G

    2012-12-01

    The influence of AME(n) concentration of the diet on productive performance and egg quality traits was studied in Hy-Line brown egg-laying hens differing in initial BW from 24 to 59 wk of age. Eight treatments were arranged factorially with 4 diets varying in energy content (2,650, 2,750, 2,850, and 2,950 kcal of AME(n)/kg) and 2 initial BW of the hens (1,733 vs. 1,606 g). Each treatment was replicated 5 times (13 hens per replicate), and all diets had similar nutrient content per unit of energy. No interactions between energy content of the diet and initial BW of the hens were detected for any trait. An increase in energy concentration of the diet increased (linear, P feed conversion ratio per kilogram of eggs (linear, P Feed intake (114.6 vs. 111.1 g/hen per day), AME(n) intake (321 vs. 311 kcal/hen per day), egg weight (64.2 vs. 63.0 g), and egg mass (58.5 vs. 57.0 g) were higher for the heavier than for the lighter hens (P feed conversion ratio per kilogram of eggs and energy efficiency were not affected. Eggs from the heavier hens had a higher proportion of yolk and lower proportion of albumen (P hens. Consequently, the yolk-to-albumen ratio was higher (P hens. It is concluded that brown egg-laying hens respond with increases in egg production and egg mass to increases in AME(n) concentration of the diet up to 2,850 kcal/kg. Heavy hens had higher feed intake and produced heavier eggs and more egg mass than light hens. However, feed and energy efficiency were better for the lighter hens.

  17. Rationale for Quality Assurance in Fecal Egg Monitoring of Soil-Transmitted Helminthiasis

    Science.gov (United States)

    Hoekendijk, David J. L.; Hill, Philip C.; Sowerby, Stephen J.

    2016-01-01

    Substantial investment has been made into the once “neglected” tropical disease, soil-transmitted helminthiasis, and into control programs that operate within a framework of mapping baseline disease distribution, measuring the effectiveness of applied interventions, establishing when to cease drug administration, and for posttreatment evaluations. However, critical to each of these stages is the determination of helminth infection. The limitations of traditional microscope-based fecal egg diagnostics have not provided quality assurance in the monitoring of parasite disease and suboptimal treatment regimes provide for the potential development of parasite resistance to anthelmintic drugs. Improved diagnostic and surveillance tools are required to protect therapeutic effectiveness and to maintain funder confidence. Such tools may be on the horizon with emergent technologies that offer potential for enhanced visualization and quality-assured quantitation of helminth eggs. PMID:27352875

  18. Effect of nanosilicon dioxide on growth performance, egg quality, liver histopathology and concentration of calcium, phosphorus and silicon in egg, liver and bone in laying quails

    Science.gov (United States)

    Faryadi, Samira; Sheikhahmadi, Ardashir

    2017-11-01

    This experiment was conducted to evaluate the effects of different levels of nanosilicon dioxide (nSiO2) on performance, egg quality, liver histopathology and concentration of calcium (Ca), phosphorus and silicon (Si) in egg, liver and bone in laying quails. The experiment was administered using 60 laying quails at 16-26 weeks of age with five treatments [0 (control), 500, 1000, 2000 and 4000 mg nSiO2 per kg of diet] and four replicates in a completely randomized design. During the experiment, the amount of feed intake was recorded weekly and performance parameters were measured. During the last 3 days of the experiment, all of the eggs in each replicate were collected and egg quality parameters were measured. At the end of 26 weeks of age, the birds were sacrificed and blood samples were collected. Liver samples from each treatment were fixed in 10% buffered formalin for histopathological assessment. The right thigh bone and a portion of liver were inserted in plastic bags and stored at - 20. The results showed that nSiO2 supplementation significantly affected egg weight and egg mass ( P 0.05) by dietary treatments. In conclusion, the results indicated that dietary supplementation of nSiO2 could improve bone density and performance without any adverse effect on the health of laying quails.

  19. The effects of egg and diacetyl tartaric acid esters of monoglycerides addition on gluten-free sorghum bread quality

    Science.gov (United States)

    The impact of whole egg addition (as is) at 20, 25, or 30% (flour basis) on sorghum bread quality was evaluated. The use of the antistaling agent diacetyl tartaric acid esters of monoglycerides (DATEM) at 0.5% (flour basis) at each of the egg addition levels was also studied. Evaluated quality facto...

  20. PRODUCTIVITY OF LAYERS AND EGG QUALITY IN FREE RANGE AND CAGE SYSTEM OF HOUSING

    Directory of Open Access Journals (Sweden)

    Đ. Senčić

    2006-12-01

    Full Text Available The research was conducted with two groups of Lohmann Brown hybrid layers. Production of eggs lasted for 52 weeks. A control group of layers was kept in the conventional housing system, that is, in cages, while experimental group was kept in the free range system. Layers from the free range system, compared to those kept in cages, laid fewer eggs, (266:295, they consumed more feed on daily basis (129 g : 115 g, more feed per kilogram of egg weight (2.83 kg : 2.35 kg, they had higher mortality rate (6.80 % : 5.50 % and lower end of lay body weight (1.95 kg : 2.10 kg. Eggs from free range layers, compared to those from the cages system, had significantly (P0.05 were determined between the free range and the cages system of housing hens. Considering somewhat lower productivity and higher mortality rate of hens, higher feed consumption per kilogram of egg mass, but also better quality of eggs, profitability of egg production in the free range system will depend, to the maximum extent, on market evaluation of the production.

  1. Nutritional Quality of Eggs of Amberlink and Hyline Layers Fed on Different Levels of Provitamin A-Biofortified Maize

    Directory of Open Access Journals (Sweden)

    GW Zeina

    Full Text Available ABSTRACT The study was conducted to determine the interaction of types of maize (Provitamin A-biofortified maize (PABM versus white maize and strain of laying birds (Amberlink and Hyline on nutritional quality of eggs. Twenty-one of each of Amberlink and Hyline laying hens were fed on three diets for 30 days. Birds were distributed in a 3 × 2 factorial arrangement constituting three diets (0, 50 or 100 % of PABM and two strains (Amberlink & Hyline. There was a diet × strain interaction on egg shell weight, average daily feed intake, egg production, egg weight and egg shell thickness. Eggs produced by layers under 100 % PABM had lighter eggshell weight and lower eggshell percentage. In contrast, eggs produced by layers under 50 % PABM diet had signi-ficantly higher eggshell percentage, heavier egg shell weight and thicker eggshell. As the level of PABM increased, the yellow and red hue (Hunter a* and b* values significantly increased while the lightness values (Hunter L* values decreased. As the level of PABM in the ration increased, the vitamin A content of the egg yolk also significantly increased. Assimilation of vitamin A from feed to egg yolk in Amberlink and Hyline hens was similar. Hence, egg enrichment with vitamin A can be achieved by using PABM in layers ration. The use of high level of PABM had a negative effect on the eggshell quality traits.

  2. Egg quality and yolk lipid composition of laying hens fed diets containing cashew nut meal

    Directory of Open Access Journals (Sweden)

    Tatiana Fontoura Vidal

    2013-03-01

    Full Text Available The objective of this study was to assess the effect of the addition of cashew nuts meal (0, 5, 10, 15, 20, and 25% to laying hen diets on egg quality and yolk composition. The variables studied were: egg weight, specific gravity, Haugh Units, percentages of shell, albumen, and yolk, moisture, total solids, total lipids, fatty acids profile, and yolk cholesterol. The addition of up to 25% of cashew nuts meal to hen diets did not affect egg quality and freshness, moisture and total solids content. However, an increase in total lipid content and a decrease in yolk pigmentation was observed. Oleic acid level increased in the yolk, whereas palmitic, stearic, and linoleic acid levels decreased. The addition of cashew nuts meal increased the monounsaturated/saturated fatty acid ratio in the yolk and reduced the cholesterol content. Therefore, the use of cashew nuts meal in laying hen diets favorably modifies the fatty acid composition of egg yolk and contributes to a better acceptance of this food by consumers since it also reduces yolk cholesterol levels.

  3. Utilization of laserpuncture induction as spawning stimulation in catfish (Clarias spp. crossbreeding toward egg quality

    Directory of Open Access Journals (Sweden)

    Pungky S.W. Kusuma

    2015-12-01

    Full Text Available The induction of laserpuncture on the reproductive acupoint of catfish can accelerate gonadotropin hormone formation from the pituitary especially gonadotropin II (GTH-II which has a role in the final stage of oocyte maturation, ovulation and spawning stimulation. The purpose of this study is to evaluate the effects of laserpuncture induction toward the egg quality from crossbreeding catfish male var. Paiton and female var. Sangkuriang. The egg quality was measured by the following parameters: fertilization rate (FR, egg hatching rate (HR, and larvae survival rate (SR. The research treatments were conducted using two levels along with eight repetitions. The results show that the crossbreed catfish using laserpuncture induction affected the parameters by increasing the mean value of fertilization rate, egg hatching rate and larvae survival rate significantly (P < 0.05 compared with mean value of fish without induction. This study concluded that laserpuncture induction on the crossbreeding between broodstock of male catfish var. Paiton and female var. Sangkuriang will increase FR, HR and SR.

  4. MicroRNA expression profiles from eggs of different qualities associated with post-ovulatory ageing in rainbow trout (Oncorhynchus mykiss)

    Science.gov (United States)

    Background: Egg quality is an important aspect in rainbow trout farming. Post-ovulatory aging is one of the most important factors affecting egg quality. MicroRNAs (miRNAs) are the major regulators in various biological processes and their expression profiles could serve as reliable biomarkers for v...

  5. An In-Depth Study on the Impact of External Quality Assurance

    Science.gov (United States)

    Stensaker, Bjorn; Langfeldt, Liv; Harvey, Lee; Huisman, Jeroen; Westerheijden, Don

    2011-01-01

    After more than two decades of external quality assurance, there is an increasing interest in questions concerning the impact and effects of this activity. Following an external evaluation of NOKUT--the Norwegian quality assurance agency, this article studies the impact of external quality assurance in detail by analysing quantitative and…

  6. Rationale for Quality Assurance in Fecal Egg Monitoring of Soil-Transmitted Helminthiasis.

    Science.gov (United States)

    Hoekendijk, David J L; Hill, Philip C; Sowerby, Stephen J

    2016-09-07

    Substantial investment has been made into the once "neglected" tropical disease, soil-transmitted helminthiasis, and into control programs that operate within a framework of mapping baseline disease distribution, measuring the effectiveness of applied interventions, establishing when to cease drug administration, and for posttreatment evaluations. However, critical to each of these stages is the determination of helminth infection. The limitations of traditional microscope-based fecal egg diagnostics have not provided quality assurance in the monitoring of parasite disease and suboptimal treatment regimes provide for the potential development of parasite resistance to anthelmintic drugs. Improved diagnostic and surveillance tools are required to protect therapeutic effectiveness and to maintain funder confidence. Such tools may be on the horizon with emergent technologies that offer potential for enhanced visualization and quality-assured quantitation of helminth eggs. © The American Society of Tropical Medicine and Hygiene.

  7. Thoughts on Internal and External Quality Assurance

    Science.gov (United States)

    Zhang, Jianxin

    2012-01-01

    Quality assurance of higher education is made up of two parts: internal quality assurance (IQA) and external quality assurance (EQA). Both belong to a union of the coexistence and balance of yin and yang. But in reality there exists a paradox of "confusion of quality assurance (QA) subject consciousness, singularity of social QA and lack of QA…

  8. EGG QUALITY AND PRODUCTIVE PERFORMANCE OF LAYING HENS IN SECOND CYCLE OF POSTURE FED WITH AMINOACID CHELATED TRACE MINERALS

    OpenAIRE

    Leticia Souza Silva Carvalho; Daniela Reis Vilela; Naiara Simarro Fagundes; Yara Lucia Silva Souza; Evandro de Abreu Fernandes

    2016-01-01

    Abstract The goal of this research was to evaluate egg quality and performance of laying hens at the second laying cycle, fed with minerals from an organic source. The  control treatment consisted of  basal diet with inorganic trace minerals, whereas the other treatments consisted of organic copper, iron, manganese, and zinc at levels of 100%, 90%, 80%, and 70%. Physical and chemical analyses were performed to evaluate egg quality. The average egg weight reduced in the test diet; however, ...

  9. Mixed crude glycerin in laying hen diets: live performance and egg quality and fatty acid profile

    Directory of Open Access Journals (Sweden)

    CRA Duarte

    2014-12-01

    Full Text Available This study evaluated the performance and the quality and fatty acid profile of eggs from laying hens fed diets containing mixed crude glycerin (MCG; 80% vegetable fat + 20% animal fat. A total of 240 39-week-old Hy-Line W36 laying hens were distributed according to a completely randomized experimental design into six treatments consisting of graded MCG dietary inclusion levels (0, 1.5, 3.0, 4.5, 6.0, and 7.5%, with five replicates of eight birds each. Feed intake linearly decreased (p<0.05 with increasing MCG inclusion levels. The percentages of myristic, palmitic, and α-linolenic acids in the eggs linearly decreased as MCG dietary levels increased (p<0.05, while α-linoleic acid, polyunsaturated fatty acids (PUFA and ω-6/ω-3 ratio linearly increased. Excreta moisture linearly increased with increasing levels of MCG inclusion (p<0.05. MCG may be included in up to 7.5% in layer feeds without impairing performance or egg quality, but levels up to 5.54% reduce SFA egg content. However, the inclusion of MCG in layer diets increases ω-6/ω-3 ratio in the eggs.

  10. Externalizing Behaviors and Callous-Unemotional Traits: Different Associations With Sleep Quality.

    Science.gov (United States)

    Denis, Dan; Akhtar, Reece; Holding, Benjamin C; Murray, Christina; Panatti, Jennifer; Claridge, Gordon; Sadeh, Avi; Barclay, Nicola L; O'Leary, Rachael; Maughan, Barbara; McAdams, Tom A; Rowe, Richard; Eley, Thalia C; Viding, Essi; Gregory, Alice M

    2017-08-01

    Sleep quality is associated with different aspects of psychopathology, but relatively little research has examined links between sleep quality and externalizing behaviors or callous-unemotional traits. We examined: (1) whether an association exists between sleep quality and externalizing behaviors; (2) whether anxiety mediates this association; (3) whether callous-unemotional traits are associated with sleep quality. Data from two studies were used. Study 1 involved 1556 participants of the G1219 study aged 18-27 years (62% female). Questionnaire measures assessed sleep quality, anxiety, externalizing behaviors, and callous-unemotional traits. Study 2 involved 338 participants aged 18-66 years (65% female). Questionnaires measured sleep quality, externalizing behaviors, and callous-unemotional traits. In order to assess objective sleep quality, actigraphic data were also recorded for a week from a subsample of study 2 participants (n = 43). In study 1, poorer sleep quality was associated with greater externalizing behaviors. This association was partially mediated by anxiety and moderated by levels of callous-unemotional traits. There was no significant relationship between sleep quality and callous-unemotional traits. In study 2, poorer sleep quality, as assessed via self-reported but not objective measures, was associated with higher levels of externalizing behaviors. Furthermore, in study 2, better sleep quality (indicated in both questionnaires and actigraphy measures: lower mean activity, and greater sleep efficiency) was associated with higher levels of callous-unemotional traits. Self-reports of poorer sleep quality are associated with externalizing behaviors, and this association is partially mediated by anxiety. Callous-unemotional traits are not associated with poor sleep and may even be related to better sleep quality. This is an exceptional finding given that poor sleep quality appears to be a characteristic of most psychopathology. © Sleep Research

  11. Effects of different levels of expanded perlite on the performance and egg quality traits of laying hens

    Directory of Open Access Journals (Sweden)

    Fatma Karakas Oguz

    Full Text Available ABSTRACT The influence of different levels of expanded perlite on the performance and egg quality traits was studied in laying hens. Forty Lohmann Brown females at 30 weeks of age were randomly assigned to four groups consisting of five replicates with two hens in each. Four diet groups were supplemented with 0% (control group, 1%, 2%, and 3% perlite, respectively. Feed and water were provided ad libitum. There were no significant differences in final body weight, feed intake, egg yield, and egg weight. Feed conversion ratio and egg weight decreased with addition of 2% perlite. Dietary perlite supplementation has no significant effects on shape, yolk, and albumen index. Haugh unit was affected significantly by addition of 2% perlite. Fecal pH, dry matter, and NH3-N did not significantly differ among treatments. Dietary perlite has no negative effect on performance and egg quality traits except 2% perlite group. Dietary expanded perlite can be added at 1% level in laying hen rations without changing the animal performance.

  12. The effects of apple pulp and probiotic on performance, egg quality traits and blood parameters of laying hens

    Directory of Open Access Journals (Sweden)

    Shabaz Noranian

    2016-11-01

    Full Text Available Introduction Apple is one of the most important fruits that is produced in the large amount in Iran. It is a good source of vitamins and minerals and active fiber. Most of the apples that product in Iran are use in food industry for producing different kinds of apple juices. After Juicing, more than 20% of apple, remain as waste. The remained matter contain considerable amount of vitamins and minerals that usually found in fresh apple, moreover it is rich source of pectin and crude fiber. Generally this byproduct discharge to environment and cause some serious environmental problems. It is thought that use of apple pulp as a part of apple waste in laying hens diets not only prevent some environmental problems, but also can improve their performance, egg quality traits, and blood biochemical parameters and reduce the production cost. The current study has been designed to investigate these traits. Materials and Methods This experiment was carried out on 192 Hi-line (W36 laying hens in a completely randomized design as (2*2 factorial arrangement with two levels of apple pulp (0 and 4% and two levels of probiotic (protexin (0 and 0.005% in 4 treatments, 4 replicates and 12 birds per replicate for 12 weeks (65-76 weeks. Results and Discussion Using apple pulp and probiotic in diets improved the egg production performance, egg quality traits and blood parameters of laying hens (P0.05. Probiotic improved egg weight, egg production percentage, egg mass, feed conversion ratio and Haugh unit. In interaction effects, using apple pulp and probiotic improved the performance and egg quality traits of laying hens. The highest egg weight, egg production, egg mass and the best feed conversion were obtained with diet containing 4% apple pulp and 0.005% probiotic. Also the highest amount of albumin, eggshell thickness and Haugh unit were observed with 4% apple pulp and 0.05% probiotic. Apple pulp decreased the blood levels of triglyceride, cholesterol and albumin

  13. Effects of dietary humic substances on egg production and egg shell ...

    African Journals Online (AJOL)

    Effects of dietary humic substances on egg production and egg shell quality of hens after peak laying period. ... Ninety Isa Brown layers from 51 to 61 weeks of age were allocated to three treatment groups, namely H0, H1 and H2. ... Egg production (% hen-day) in the H2 group was higher (P < 0.05) than control group.

  14. [Standardization in laboratory hematology by participating in external quality assurance programs].

    Science.gov (United States)

    Nazor, Aida; Siftar, Zoran; Flegar-Mestrić, Zlata

    2011-09-01

    Since 1985, Department of Clinical Chemistry and Laboratory Medicine, Merkur University Hospital, Zagreb, has been participating in the International External Quality Assessment Scheme for Hematology (IEQAS-H) organized by the World Health Organization (WHO). Owing to very good results, in 1987 the Department received a certificate of participation in this control scheme. Department has been cooperating in the external quality assessment program in laboratory hematology which has been continuously performed in Croatia since 1986 by the Committee for External Quality Assessment Schemes under the auspices of the Croatian Society of Medical Biochemists and School of Pharmacy and Biochemistry, University of Zagreb. Nowadays, 186 medical biochemical laboratories are included in the National External Quality Assessment program, which is performed three times per year. Our Department has participated in the international projects of the European Committee for External Quality Assurance Programs in Laboratory Medicine (EQALM).

  15. Effect of dietary protein sources on production performance, egg quality, and plasma parameters of laying hens

    Directory of Open Access Journals (Sweden)

    Xiaocui Wang

    2017-03-01

    Full Text Available Objective This study was conducted to evaluate the effects of dietary protein sources (soybean meal, SBM; low-gossypol cottonseed meal, LCSM; double-zero rapeseed meal, DRM on laying performance, egg quality, and plasma parameters of laying hens. Methods A total of 432 32-wk-old laying hens were randomly divided into 6 treatments with 6 replicates of 12 birds each. The birds were fed diets containing SBM, LCSM100, or DRM100 individually or in combination with an equal amount of crude protein (CP (LCSM50, DRM50, and LCSM50-DRM50. The experimental diets, which were isocaloric (metabolizable energy, 11.11 MJ/kg and isonitrogenous (CP, 16.5%, had similar digestible amino acid profile. The feeding trial lasted 12 weeks. Results The daily egg mass was decreased in the LCSM100 and LCSM50-DRM50 groups (p0.05 and showed increased yolk color at the end of the trial (p0.05. Conclusion Together, our results suggest that the LCSM100 or DRM100 diets may produce the adverse effects on laying performance and egg quality after feeding for 8 more weeks. The 100.0 g/kg LCSM diet or the148.7 g/kg DRM diet has no adverse effects on laying performance and egg quality.

  16. Effect of Feeding and Withdrawal of Vanadium and Vitamin C on Egg Quality and Vanadium Residual Over Time in Laying Hens.

    Science.gov (United States)

    Wang, J P; He, K R; Ding, X M; Bai, S P; Zeng, Q F; Zhang, K Y

    2017-06-01

    This experiment examined the egg quality of hens fed vanadium (V) and vitamin C (VC) during storage, as well as the V and VC withdrawal on egg quality and V residual in egg. A total of 360 laying hens (31 weeks old) were randomly allotted into a 3 × 2 factorial arrangement treatments (6 replicates and 10 chicks per replicate) with three levels of dietary V (0, 5, and 10 mg/kg) and two levels of VC (0 and 100 mg/kg) for 19 weeks (feeding V and VC 12 weeks, recovery 7 weeks). The V residual in eggs at 4, 8, and 12th weeks were increased (linear effect, P ≤ 0.01) as V levels increased and was not detected in albumen at 7th week after V withdrawal. Followed by 12-week feeding period, albumen height and Haugh unit of eggs during 2-week storage were decreased (linear and quadratic effect, P effect, P effect, P effect, P effect on albumen height and HU continued to be observed until the seventh week, whereas the bleaching effect on eggshell color disappeared after 1-week withdrawal. The results indicated that feeding 5 or 10 mg/kg V increases egg V residual and reduces egg albumen quality and bleached the shell color, and the impaired albumen quality induced by 10 mg/kg of V lasted at least 6 weeks after changing to no V supplementation diet. The addition of VC did not show to affect egg quality during storage or recovery phase.

  17. The effect of phytobiotics, organic acids and humic acids on the utility and egg quality of laying hens

    Directory of Open Access Journals (Sweden)

    Henrieta Arpášová

    2017-11-01

    Full Text Available The aim of this study was the assessment of an influence of supplement of dietary herbal additive in combination with organic acids into feed mixture or drinking water of laying hens on performance parameters and egg quality. The Lohmann Brown Lite laying hens (n = 30 were divided into 3 groups (n = 10, and fed for 20 weeks ad libitum with complete feed mixtures (CFM. Hens in the control group received the complete feed mixture (CFM and drank drinking water without any supplements. In the first experimental group hens received CFM without supplements but phytobiotics (bergamot oil (Citrus bergamia, thyme (Thymus vulgaris, clove (Syzygium aromaticum, pepper (Piper nigrum in combination with the fumaric acid and citric acid at 60 mg per 1 liter of water were added to their drinking water. In the second experimental group was CFM enriched with humic acids in the concentration of 0.5%, and phytobiotcs with organic acids at the same dose as in the first experimental group were added to their drinking water. Monitored parameters: body weight (g, egg production (%, the weight of all produced eggs (g, egg albumen weight (g, egg albumen index, Haugh unit (HU, egg yolk weight (g, egg yolk index, egg yolk colour (° HLR, egg shell weight (g and egg shell strength (N.cm-2. The results showed no significant differences between the both experimental groups and the control group in the parameter body weight of hens (P>0.05. The highest average body weight was found in the hens from the second experimental group (values in the order of groups:  1792.22 ± 80.85; 1768.42 ±55.55; 1820.12 ±78.56 g±S.D.. We observed positive trend of increasing of egg production by adding of used supplements, especially in the second experimental group with the addition of humic acids, although with no statistically significant difference compared to the control group (P>0.05. The mean laying intensity in the order of groups: 90.42; 91.16; 91.56%. We observed statistically

  18. The Effect of Calcium and Available Phosphorus Levels on Performance, Egg Quality and Bone Characteristics of Japanese Quails at End of the Egg-Production Phase

    Directory of Open Access Journals (Sweden)

    CLN Ribeiro

    Full Text Available ABSTRACT Calcium (Ca and available phosphorus (avP requirements for egg production are different between the beginning and the end of the egg-production cycle. The objective of this study was to estimate the Ca and avP requirements of Japanese quails at end of the production phase. In total, 256 Japanese quails with 48 weeks of age were randomly distributed in 2 x 2 factorial arrangement consisting of two Ca levels (29 and 38 g/kg and two avP levels (1.5 and 3.0 g/kg, totaling four treatments with eight replicates of eight hens each. The variables evaluated were feed intake (FI; egg production (EP; marketable egg production (MEP; egg weight (EW; egg mass (EM; feed conversion ratio per egg mass (FCRM; feed conversion ratio per dozen eggs (FCRD; livability (L; yolk weight (YW, albumen weight (AW, eggshell weight (SW; yolk percentage (YP, albumen percentage (AP, eggshell percentage (SP, specific egg weight (SEW; bone calcium percentage (BCa, bone phosphorus percentage (BP, bone ash weight (Bash and bone ash percentage (PBash. There was no interaction between Ca and avP levels for any of the evaluated parameters, except for SEW. Quail performance was not influenced by the treatments, except for MEP. Bone characteristics were not influenced by the tested dietary Ca and avP levels. However, SW and SP increased and AP decreased as dietary Ca level increased. The dietary supplementation of 38 g Ca/kg and 3.0 g avP/kg may increase marketable egg production and the egg quality of Japanese quails at end of the production cycle.

  19. Oviposition and egg quality traits of dwarf and naked neck layers ...

    African Journals Online (AJOL)

    Oviposition and egg quality traits were studied in dwarf and naked neck layers in Maputo (Mozambique) during a 28-d period at 35 weeks of age. Birds were caged individually in a laying house with natural light and ventilation. Average daylight length during the study was 11.2 hr and minimum and maximum temperature ...

  20. Quality characteristics of egg-reduced pound cakes following WPI and emulsifier incorporation

    NARCIS (Netherlands)

    Paraskevopoulou, A.; Donsouzi, S.; Nikiforidis, C.V.; Kiosseoglou, V.

    2015-01-01

    The effect of partial (50 wt%) or total liquid egg replacement by whey proteins in combination with emulsifiers, i.e. hydroxypropylmethylcellulose (HPMC) and sodium stearoyl-2-lactylate (SSL), on the quality of pound cakes was investigated. Cakes containing whey protein isolate (WPI) solutions of

  1. The effect of dietary protein and lysine on egg quality and production of laying hens during 28-42 weeks of age

    Directory of Open Access Journals (Sweden)

    Hasan Mohammadi Emarat

    2015-04-01

    Full Text Available An experiment was conducted to evaluate the effect of dietary crude protein and lysine levels on quality and quantity of egg production. Fifteen diets consisted of 3 levels of protein (14, 15 and 16% and 5 levels of lysine (0.71, 0.74, 0.77, 0.80 and 0.83 % in a 35 factorial arrangement were provided. Each diet was randomly fed to 4 replicates of 12 birds, during four periods of 4 weeks (28-44wks of age. Egg number and mortality was recorded daily, whereas feed consumption determined for each period. Eggs from each replicate were weighed at the end of three consecutive days of each period and six eggs were used to measure the egg quality characteristics. Although the feed intake did not affected by dietary protein but the egg production, egg mass and feed conversion were improved significantly (p

  2. Quantification of egg proteome changes during fertilization in sterlet Acipenser ruthenus.

    Science.gov (United States)

    Niksirat, Hamid; Andersson, Liselotte; Golpour, Amin; Chupani, Latifeh; James, Peter

    2017-08-19

    Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects of different activation media including freshwater and clay suspension on protein abundances of egg were quantified in sterlet Acipenser ruthenus. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification in the eggs of five females. No significant (p > 0.05) difference was found between protein abundances in eggs activated with different media. However, results showed significant (p eggs as control. The fact that abundance of proteasome subunit alpha significantly reduced only in eggs which were activated by clay suspension suggests that activation medium can somehow intervene with protein regulation during fertilization. In conclusion, external fertilization in sturgeon egg is accompanied by huge release of proteins into the external environment that may participate in the construction of a transient microenvironment around egg for attraction and protection of spermatozoa to ensure ensuing fertilization. Data are available via ProteomeXchange with identifier PXD006232. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. The effect of the clove essential oil to the production and quality of lyaing hens eggs

    Directory of Open Access Journals (Sweden)

    Henrieta Arpášová

    2017-05-01

    Full Text Available The aim of this study was to determinate the effect of the clove (Syzygium aromaticum essential oil to the egg production parameters and egg quality of laying hens. A total of 40 Lohmann Brown laying hens were divided into 4 groups (n = 10. The laying hens were fed 20 weeks ad libitum. The control group was fed with complete feed mixtures without additives. The first group of hens was fed a diet with complete feed mixtures with 0.3 mg.kg-1 of clove essential oil. The second group of hens was fed a diet with complete feed mixtures with 0.6 mg.kg-1 of clove oil and the third group of hens was fed a diet with complete feed mixtures with 1 mg.kg-1 of clove oil. Egg production, egg weight (g, specific egg weight, albumen weight (g, albumen index, Haugh Units (HU, yolk weight (g, yolk index, yolk color (HLR were studied. The results show that the addition of clove oil, especially in the group of laying hens with the addition of 1 mg.kg-1 clove oil had no significantly different (P>0.05 to the egg production (the order of the groups: 90.3; 91.8; 91.3; 92.6 %. In egg weight were found no significant differences between groups. The addition of 1 mg.kg-1 clove oil had significant (P>0.05 influenced the albumen index and the addition of 0.6 mg.kg-1 and 1 mg.kg-1 clove oil had significant (P>0.05 influenced Haugh units. In the control group was found not significant (P>0.05 different in yolk weight compared with other groups. In the other parameters of egg quality (albumen weight, yolk index, yolk colour with the addition of clove oil were found no significant differences between groups.

  4. The effects of dietary calcium iodate on productive performance, egg quality and iodine accumulation in eggs of laying hens.

    Science.gov (United States)

    Bakhshalinejad, R; Hassanabadi, A; Nassiri-Moghaddam, H; Zarghi, H

    2018-01-25

    The aim of this study was to examine the effects of various levels of supplemental calcium iodate (CI) on productive performance, egg quality, blood indices and iodine (I) accumulation in the eggs in commercial laying hens. A total of 240 White Leghorn layers (Hy-line W36) were divided through a completely randomized design into six treatments with five replicates and eight hens per each at 32 weeks of age. This experiment lasted for 12 weeks. Concentrations of I in the mash diets were 0.74, 3.13, 5.57, 8.11, 10.65 and 12.94 mg I/kg of feed in treatments 1-6 respectively. The added doses of CI were included 0.0 (control), 2.5, 5.0, 7.5, 10.0 and 12.5 mg/kg of diet for treatments 1-6 respectively. There were no significant differences in productive performance among the treatments. The highest eggshell strength was observed in group fed diet containing 3.13 mg I/kg (p = .014). The highest percentage of calcium and lowest percentage of phosphorus in eggshell were observed in group fed diet containing 12.94 mg I/kg (p = .0001). Feeding hens with diet containing 12.94 mg I/kg increased serum triiodothyronine-to-thyroxine ratio (p = .0001). Serum alanine aminotransferase activity in hens fed diet containing 12.94 mg I/kg was significantly more than control (p = .041). Blood Serum triglycerides in hens fed diet containing 8.11 mg I/kg were significantly higher than control (p = .0001). Edible fraction of the eggs of birds fed diet containing 12.94 mg I/kg was enriched by I almost 3 times more than those fed diet containing 0.74 mg I/kg. The results suggested that egg production, egg mass, feed intake and feed conversion ratio were not significantly affected by dietary I levels. Iodine accumulation in the eggs were increased by increasing dietary I levels and the level of 10 mg/kg CI could supply I enrichment of the eggs. © 2018 Blackwell Verlag GmbH.

  5. The quality of eggs (organic and nutraceutical vs. conventional) and their technological properties.

    Science.gov (United States)

    Filipiak-Florkiewicz, Agnieszka; Deren, Katarzyna; Florkiewicz, Adam; Topolska, Kinga; Juszczak, Leslaw; Cieslik, Ewa

    2017-07-01

    The aim of the study was to compare the quality of commercially available eggs: organic (attested organic farm) - OE, nutraceutical (enriched in n-3 fatty acids) - NE, vs. conventional (cage raising system) - CE and their technological (emulsifying) properties. Their basic chemical composition (i.e., moisture, protein, fat, ash) as well as mineral (Na, K, Ca, Mg, Zn, Fe, Mn, Cu) contents were assessed, and fatty acid profiles were determined. Next, the suitability of the eggs for mayonnaise production was assessed.The yolk of organic eggs contained the highest protein (17.7 g/100 g), K (134.7 mg/kg), and Cu (0.15 mg/kg) levels. Meanwhile, the yolk of conventional eggs was the most abundant in Mg (14.6 mg/kg) and Fe (9.05 mg/kg), and the nutraceutical ones - in Ca (109.0 mg/kg) as well as Mn (0.26 mg/kg). Albumen of organic eggs contained the highest level of protein (13.0 g/100 g), whereas the nutraceutical ones contained the highest levels of - ash (1.2 g/100 g), K (150.2 mg/kg), Zn (0.11 mg/kg), and Mn (0.04 mg/kg). Nutraceutical eggs contained a higher amount of n-3 and monounsaturated fatty acids but also smaller saturated fatty acids content. This beneficial fatty acids profile is particularly important from the nutritional point of view. Despite the lack of differences in emulsifying properties among the studied eggs, the mayonnaises prepared on the basis of the nutraceutical eggs showed better stability. This attribute is very important for both the producer (during product transport) and the consumer (during dish preparation). © 2017 Poultry Science Association Inc.

  6. Exploratory and descriptive study on nutritional characteristics and quality of eggs from Chilean partridge (Nothoprocta perdicaria).

    Science.gov (United States)

    Arias, José Luis; Matthei, Alberto; Valenzuela, Carolina

    2018-01-01

    This work aims to contribute more information on tinamou eggs by performing an exploratory and descriptive study of some of their nutritional and quality characteristics. The chemical composition of tinamou egg showed a high protein content in white (85% dry basis) and high lipid concentration in yolk (52% db). The iron (Fe) content in white was higher than hen egg (0.47 mg/100 g) and this could be associated with the observed pinkish color of the white. As in the hen egg, the major fatty acids in tinamou yolk were: oleic (39%), linoleic (23%) and palmitic (20%). The cholesterol content of tinamou was 21.2 mg/g of yolk, and 100 g of whole egg provides 589 mg of cholesterol. As in the hen egg, tinamou egg white showed high levels of lysine, sulfur-containing amino acids, threonine and valine with respect to the recommended allowance for an adult man. All essential amino acids with the exception of histidine cover the adult requirements. The shell inorganic composition of these eggs is calcium carbonate and the morphology was similar to other avian eggs. Tinamou egg is small and elongated, with a dark brown color. The eggshell is thinner and experiences more deformation but less breaking strength than hen eggshell. © 2017 Japanese Society of Animal Science.

  7. Minimal requirements for quality controls in radiotherapy with external beams

    International Nuclear Information System (INIS)

    1999-01-01

    Physical dosimetric guidelines have been developed by the Italian National Institute of Health study group on quality assurance in radiotherapy to define protocols for quality controls in external beam radiotherapy. While the document does not determine strict rules or firm recommendations, it suggests minimal requirements for quality controls necessary to guarantee an adequate degree of accuracy in external beam radiotherapy [it

  8. Feed addition of curcumin to laying hens showed anticoccidial effect, and improved egg quality and animal health.

    Science.gov (United States)

    Galli, Gabriela M; Da Silva, Aleksandro S; Biazus, Angelisa H; Reis, João H; Boiago, Marcel M; Topazio, Josué P; Migliorini, Marcos J; Guarda, Naiara S; Moresco, Rafael N; Ourique, Aline F; Santos, Cayane G; Lopes, Leandro S; Baldissera, Matheus D; Stefani, Lenita M

    2018-01-31

    The aim of this study was to evaluate whether the addition of curcumin in the diet of commercial laying hens could have an anticoccidial action and improve egg quality. For this, 60 laying hens were divided into three groups: T0 (the control group); T30 and T50 (30 and 50 mg/kg of curcumin in the feed, respectively). Eggs recently laid were collected on days 14 and 21 of the experiment, and stored for 21 days. It was observed increased specific gravity and yolk index in stored eggs of the groups T30 and T50 compared to T0. The yolk color reduced in the eggs stored from groups T30 and T50 compared to T0. Moreover, TBARS levels were lower in fresh and stored eggs from groups T30 and T50. It was observed increased TAC levels in fresh eggs from groups T30 and T50 and in stored eggs from the group T50. The presence of curcumin was not detected by HPLC in the yolk and albumen. Seric levels of albumin and uric acid did not differ between groups, while seric levels of total proteins increased on day 21 on groups T30 and T50. Finally, it was observed a significant reduction on the number of oocysts in fecal samples on days 14 and 21 of T30 and T50 compared to T0. Based on these evidences, it is possible to conclude that the addition of curcumin in the diet of laying hens has an anticoccidial effect and improves egg quality. Copyright © 2018 Elsevier Ltd. All rights reserved.

  9. Effect of cage tier and age on performance, egg quality and stress ...

    African Journals Online (AJOL)

    This study was conducted to investigate the effects of cage tier and age on performance characteristics of layer hybrids, egg quality and some stress parameters. Ninety laying hens (hybrid ATAK-S) of similar bodyweights were used in the experiment. They were housed in three-tier conventional battery cages (bottom, ...

  10. Effects of two different rearing systems (organic and barn on production performance, animal welfare traits and egg quality characteristics in laying hens

    Directory of Open Access Journals (Sweden)

    Luigi Guidobono Cavalchini

    2010-01-01

    Full Text Available Alternative housing systems for hen eggs production represents clear evidence of the trend in animal housing and husbandry towards extensive rearing methods. Consumer demand is oriented towards healthy foods controlled not only under a safety point of view, but also under a welfare assessment of the animals’ living conditions. Among the different alternative systems deep litter and organic production in recent years have been improved in Italy. The aim of this study was to evaluate whether different housing systems (barn B and organic O for laying hens may influence productive performance, fear responses and egg quality characteristics. A total of 4,745 birds were housed in the B system and 2,016 in the O system, both of which were commercial facilities. In each system the same strain (Hy-Line Brown was housed and layer performance, external and internal egg characteristics, mortality and feed consumption were recorded weekly. Animal reactivity was recorded monthly with the approaching test. Moreover, the Tonic Immobility test was conducted at 70 weeks of age; feather and foot pad conditions were also investigated at the same time. The peak of laying was reached in both housing systems at 25 weeks of age and was higher in organic hens (94.5% than in barn hens (93.0%. Feed conversion rate during the overall laying period was 2.36 vs 2.20, respectively, in O and B housing systems. There was a significant difference concerning the eggs classified as very dirty, dirty and cracked between the two systems. The dirty eggs were higher in O system probably due to laying eggs in a free range area, while the higher number of cracked eggs in B system may be due to a significantly less shell thickness in this system. Egg weight increased with layer age in both housing systems. Animals reared in O system showed less fearfulness than in B emphasised by the approaching and Tonic Immobility test results. Feather scoring did not evidence any severe plumage

  11. Effects of packaging, mineral oil coating, and storage time on biogenic amine levels and internal quality of eggs.

    Science.gov (United States)

    Figueiredo, T C; Assis, D C S; Menezes, L D M; Oliveira, D D; Lima, A L; Souza, M R; Heneine, L G D; Cançado, S V

    2014-12-01

    This study was carried out with the aim of evaluating the effects of mineral oil application on eggshells and the use of plastic packages with lids on the physical-chemical and microbiological quality and biogenic amine contents of eggs stored under refrigeration for up to 125 d. A total of 1,920 eggs from 46-wk-old Hyline W36 laying hens were randomly distributed into 4 groups soon after classification: (i) 480 eggs were stored in pulp carton tray packages; (ii) 480 eggs were stored in plastic packages with lids; (iii) 480 eggs were stored in carton packages after the application of mineral oil; and (iv) 480 eggs were stored in plastic packages with lids after the application of mineral oil. The internal quality was measured by Haugh units, by the counts of mesophilic and psychrotrophic microorganisms, by the most probable number of total and thermal-tolerant coliforms, by the counts of molds and yeasts, by the analysis of Salmonella spp. and Staphylococcus spp., and by the levels of biogenic amines in the egg yolk and albumen. The application of mineral oil to the eggshell resulted in higher Haugh unit values throughout storage, and the use of plastic packages altered the internal quality. The application of mineral oil and the use of packaging had no effects on the microbiological and biogenic amine results. Microbiological analyses showed the absence of Salmonella spp., Staphylococcus aureus, thermal-tolerant coliforms, and fungi. However, the highest counts of mesophilic (1.1 × 10(7) cfu/g) and psychrotrophic (6.7 × 10(7) cfu/g) microorganisms were recorded. The highest values of biogenic amines detected and quantified were putrescine (2.38 mg/kg) and cadaverine (7.27 mg/kg) in the egg yolk and putrescine (1.95 mg/kg), cadaverine (2.83 mg/kg), and phenylethylamine (2.57 mg/kg) in the albumen. Despite these results, the biogenic amine levels recorded were considered low and would not be harmful to consumer health. ©2014 Poultry Science Association Inc.

  12. Substitution of Soybean Meal with Indigofera zollingeriana Top Leaf Meal on Egg Quality of Cortunix cortunix japonica

    Directory of Open Access Journals (Sweden)

    F. Faradillah

    2015-12-01

    Full Text Available This research aimed to study the substitution of soybean meal (SBM with Indigofera zollingeriana top leaf meal (ILM in the diet on egg quality of Japanese quails. The experiment used a completely randomized design with five treatments and four replications (ten quails of each replication. The dietary treatment contained five combination of SBM and ILM, R0= diets contained 18% SBM without ILM, R1= diet contained 16.2% SBM and 2.66% ILM, R2= diet contained 14.4% SBM and 5.32% ILM, R3= diet contained 12.6% SBM and 7.98% ILM, R4= diet contained 9% SBM and 13.3% ILM. The results showed that the use of 13.3% ILM (R4 significantly (P<0.05 increased feed consumption, egg weight, yolk colour score, egg cholesterol, and reduced malondialdehyde level. The conclusion of this study was I. zollingeriana top leaf meal could be used as much as 13.3% in the diets. The use of I. zollingeriana top leaf meal could improve the quality of eggs physically and chemically.

  13. The improvement of eggs quality of Mojosari duck (Anas javanica with soybean husk fermentation using cellulolytic bacteria of Spodoptera litura

    Directory of Open Access Journals (Sweden)

    Sri Hidanah

    2018-05-01

    Full Text Available Aim: This study was aimed to improve the quality of the eggs of Mojosari duck (Anas javanica through complete feeding containing soybean husk was fermented using cellulolytic bacteria of Spodoptera litura. Materials and Methods: This study consisted of three stages: The first stages, isolation and identification of cellulolytic bacteria from S. litura; the second stage, the fermentation of soybean husk through the application of bacterial cellulolytic isolate from the first stage; and the third stage, the application of the best complete feed formulation from the second stage to Mojosari duck. Results: There are four dominant bacteria: Bacillus sp., Cellulomonas sp., Pseudomonas sp., and Cytophaga sp. Furthermore, the best reduction of the crude fiber of soybean husks is the use of Cellulomonas sp. bacteria. The final of the study, the quality of the eggs of Anas javanica, was improved, as indicated by cholesterol decrease from the yolk without the decrease of egg weight and eggshell thickness, although the decrease in egg yolk color was inevitable. Conclusion: Soy husk fermentation using cellulolytic bacteria of S. litura was added to complete feeding can be performed to improve the quality of the eggs of Mojosari duck.

  14. [Quality in Revision Arthroplasty: A Comparison between Claims Data Analysis and External Quality Assurance].

    Science.gov (United States)

    Wessling, M; Gravius, S; Gebert, C; Smektala, R; Günster, C; Hardes, J; Rhomberg, I; Koller, D

    2016-02-01

    External quality assurance for revisions of total knee arthroplasty (TKA) and total hip arthroplasty (THA) are carried out through the AQUA institute in Germany. Data are collected by the providers and are analyzed based on predefined quality indicators from the hospital stay in which the revision was performed. The present study explores the possibility to add routine data analysis to the existing external quality assurance (EQS). Differences between methods are displayed. The study aims to quantify the benefit of an additional analysis that allows patients to be followed up beyond the hospitalization itself. All persons insured in an AOK sickness fund formed the population for analysis. Revisions were identified using the same algorithm as the existing external quality assurance. Adverse events were defined according to the AQUA indicators for the years 2008 to 2011.The hospital stay in which the revision took place and a follow-up of 30 days were included. For re-operation and dislocation we also defined a 365 days interval for additional follow-up. The results were compared to the external quality control reports. Almost all indicators showed higher events in claims data analysis than in external quality control. Major differences are seen for dislocation (EQS SD: 1.87 vs. claims data [cd] SD: 2.06 %, cd+30 d: 2.91 %, cd+365 d: 7.27 %) and reoperation (hip revision: EQS SD: 5.88 % vs. claims data SD: 8.79 % cd+30 d: 9.82 %, cd+365 d: 15.0 %/knee revision: EQS SD: 3.21 % vs. claims data SD: 4.07 %, cd+30 d: 4.6 %, cd+365 d: 15.43 %). Claims data could show additional adverse events for all indicators after the initial hospital stay, rising to 77 % of all events. The number of adverse events differs between the existing external quality control and our claims data analysis. Claims data give the opportunity to complement existing methods of quality control though a longer follow-up, when many complications become evident. Georg

  15. Effects of Deinococcus spp. supplement on egg quality traits in laying hens.

    Science.gov (United States)

    Li, I-Chen; Wu, Szu-Yin; Liou, Jenn-Fa; Liu, Hsiao-Hui; Chen, Jiau-Hua; Chen, Chin-Chu

    2018-01-01

    To counter the ill effects of synthetic dyes, bacterial pigment production as an alternative is now one of the promising and emerging fields of research. This study was conducted to evaluate the applicability of Deinococcus genus on the egg quality traits in laying hens. In study I, 24 single comb White Leghorn layers were fed with various 1 wt % Deinococcus bacterial strains for 10 d. In study II, 84 brown Hendrix layers were fed with one of 4 diets containing 0, 0.2, 1, or 5 wt % Deinococcus sp. GKB-Aid 1995 powder for 12 wk. In study III, 60 White Leghorn laying hens were fed either with or without 1 wt % Deinococcus sp. GKB-Aid 1995 powder, 1 wt % Deinococcus sp. GKB-Aid 1995 granules, or 1 wt % Deinococcus sp. GKB-Aid 1995 oily granules for 10 successive d. In all of the experiments, feeding Deinococcus powder did not affect egg quality traits except for the yolk color. In particular, supplementation with all Deinococcus powder treatments changed the yolk color (P 1995. Moreover, longer supplementation of Deinococcus sp. GKB-Aid 1995 in study II had a significant effect on feed conversion ratio. With these findings under consideration, the present study suggests that the Deinococcus species, especially Deinococcus sp. GKB-Aid 1995, can be an excellent candidate for improving egg yolk color in laying hens. © 2017 Poultry Science Association Inc.

  16. Rearing conditions determine offspring survival independent of egg quality : A cross-foster experiment with Oystercatchers Haematopus ostralegus

    NARCIS (Netherlands)

    van de Pol, Martijn; Bakker, T; Saaltink, DJ; Verhulst, S; Saaltink, Dirk-Jan

    Variation in rearing conditions, due either to parental or to environmental quality, can result in offspring of different quality (e.g. body condition, immune function). However, evidence is accumulating that egg size and composition can also affect offspring quality. In Oystercatchers Haematopus

  17. Sensory quality and energy use for scrambled eggs and beef patties heated in institutional microwave and convection ovens

    Energy Technology Data Exchange (ETDEWEB)

    Cremer, M.L.

    1982-05-01

    Scrambled eggs (96 portions) and beef patties (96 portions) were heated in institutional microwave and convection ovens to determine energy use in heating and sensory quality of food. For both products, significantly (P < 0.01) more energy (BTU) was used for heating in the convection than in the microwave oven and respective amounts (BTU) were 28658.7 and 9385.7 for eggs; 31313.3 and 9365.0 for beef patties. All scores for sensory quality were significantly (P < 0.01) higher for eggs heated in the microwave than in the convection, but for beef patties, scores were higher for all characteristics and significantly (P < 0.01) higher for appearance, flavor, and general acceptability after heating in the convection rather than the microwave oven.

  18. Effects of Enzyme Supplementation on Productive Performance and Egg Quality of Laying Hens fed Diets Containing Graded Levels of Whole Date Waste

    Directory of Open Access Journals (Sweden)

    Torki M

    2014-12-01

    Full Text Available The objective of present study was to determine the effects of β-mannanase-based enzyme (Hemicell® on productive performance and egg quality in diets containing graded levels of Whole date waste (WDW fed to laying hens. A total of 336 Hy-line leghorn hens after production peak were randomly divided into 56 cages. Eight iso-energetic and iso-nitrogenous experimental diets in a 4 × 2 factorial arrangement including four levels of WDW (0, 10, 20 and 30% and 2 concentrations of supplemental β-mannanase (0 or 0.06 % were prepared. Each dietary treatment was fed to 7 cages (6 birds/cage from 32 to 38 wk of age. During the experiment, daily egg production, egg weight and feed intake were measured. At the 6th wk, egg quality traits were also recorded. The results showed that there was no interaction between WDW inclusion and enzyme supplementation on performance and egg traits. Dietary supplementation of WDW more than 10% significantly decreased egg production and egg mass compared to no WDW recipient hens (control diet during the entire experiment (P. Inclusion of 30% WDW to the diet, significantly increased overall feed conversion ratio compared to the control group (P. The treatment with 20 and 30% WDW also resulted in lower eggshell thickness as compared to 10% WDW (P. The dietary inclusion of 10% WDW also increased yolk index as compared to the control and 30% WDW groups (P. Enzyme supplementation had no significant effect on productive performance as well as egg quality characteristics. Based on the results of this experiment, it can be concluded that WDW could be included to laying hens diets up to 10% with no deleterious effects on performance and egg quality characteristics.

  19. The Effects of Hyperthyroidism and Hypothyroidism Over the Egg Quality in Japanese Quails (Coturnix coturnix Japonica)

    OpenAIRE

    ÖZCAN, Mukaddes; ÇÖTELİOĞLU, Ülker

    1998-01-01

    In this study, the influence of hyperthyroidism and hypothyroidism on the egg quality in 5 week-female Japanese quails was investigated. Hyperthyroidsm and hypothyroidism were achieved by mixing 0.4 mg/100 g. levatrocsyn-sodium and % 0.2 g. propylthiouracil (PTU) in to the food during the 5 weeks period, respectively. It was found that egg weight, albumen weight, shell weight and shell thickness values in both trial groups reduced (P

  20. Effect of long-term selection for egg production on eggshell quality of Japanese quail (Coturnix japonica).

    Science.gov (United States)

    Fathi, M M; El-Dlebshany, A E; El-Deen, M Bahie; Radwan, L M; Rayan, G N

    2016-11-01

    An experiment was conducted to evaluate egg quality and ultrastuctural measurements of eggshell using a Scanning Electron Microscope (SEM) in 2 lines (selected and control) of Japanese quail. A selection program was applied over 22 consecutive generations for higher egg production and lower broken egg percentage. The results revealed that the females of the selected line produced significantly (P < 0.01) higher egg mass compared to that of the control line. Also, the selection procedure significantly improved feed conversion ratio. The eggshells of the selected line had a higher breaking strength compared to those of the control line, although there was no difference between them in shell thickness. Significantly higher wet (P < 0.01) and dry (P < 0.05) eggshell percentages were found in the selected line. In general, the eggshells of the selected line had a lower total score (good) of ultrastructural evaluation compared to the control line. According to scanning electron microscope data, the incidence of certain structural variants was more common in eggshells of the control line suggesting poor shell strength. The incidence of alignment was more prevalent in control eggshells compared to selected ones, suggesting lower resistance to breakage. Late fusion and large interstitial spaces of the palisade layer indicating decreased resistance to fracture were observed in control eggshells. It could be concluded that the improvement eggshell quality may be caused by the long-term selection for lower cracked and broken egg rates from generation to generation. © 2016 Poultry Science Association Inc.

  1. RESEARCH REGARDING SOME PHYSICAL QUALITY VALUES OF JAPANESE QUAIL EGGS OBTAINED AT THE MIDDLE OF THE LAYING STAGE

    Directory of Open Access Journals (Sweden)

    ANCA TEUŞAN

    2009-10-01

    Full Text Available The Japanese quail is one of the smallest bird subspecies which has been tamed by man, being industrially raised in our days in many states over the world. This avian subspecies produces over 300 eggs, and though there are about 6 races and different varieties, there are very little data in the scientific literature regarding the quality values for eggs of Japanese quail hatching eggs collected from hens at the end of the laying – physical values (pH value of the eggs components, albumen index, yolk index The present paper wants to bring a series of data regarding some of these indicators. The pH values was determined with an portable pH-oximeter, and to establish the other two quality indexes we used a device fitted with callipers taking into account: height of the dense egg white (albumen, minimum and maximum diameter of the dense and fluid egg white, height and the diameter of the yolk. The albumen pH recorded values of 8.90, and the yolk pH values of 6.07. The statistical mean for the 130 values of albumen index taken in study was 0.048. The mean value of yolk index was 0.399. The obtained results are normal for the period of quail’s life.

  2. Comparison of two different breeding systems laying hens in relation to egg shell quality, II

    OpenAIRE

    Mária Angelovičová; Viera Ševčíková; Marek Angelovič; Ondřej Bučko

    2014-01-01

    The aim of work was to follow up and statistically evaluate the selected quality indicators of egg shell according to two different breeding systems and different age of laying hens. An object of investigation were shell weight, share of the shell, strength and thickness of the shell for table eggs. There were used the laying hens of final hybrid ISA Brown reared in enriched cage system, and free range system. In both breeding systems were ensured the conditions with application of the welfar...

  3. The physiology and toxicology of salmonid eggs and larvae in relation to water quality criteria

    International Nuclear Information System (INIS)

    Finn, Roderick Nigel

    2007-01-01

    The purpose of this review is to collate physiological knowledge on salmonid eggs and larvae in relation to water quality criteria. Salmonid genera reviewed include Coregonus, Thymallus, Salvelinus, Salmo, and Oncorhynchus spp. When physiological data for salmonids are lacking, the zebrafish and medaka models are included. The primary focus is on the underlying mechanisms involved in the hydro-mineral, thermal, and respiratory biology with an extended section on the xenobiotic toxicology of the early stages. Past and present data reveal that the eggs of salmonids are among the largest shed by any broadcast spawning teleost. Once ovulated, the physicochemical properties of the ovarian fluid provide temporary protection from external perturbations and maintain the eggs in good physiological condition until spawning. Following fertilisation and during early development the major structures protecting the embryo from poor water quality are the vitelline membrane, the enveloping layer and the chorion. The vitelline membrane is one of the least permeable membranes known, while the semi-permeable chorion provides both physical and chemical defense against metals, pathogens, and xenobiotic chemicals. In part these structures explain the lower sensitivity of the eggs to chemical imbalance compared to the larvae, however the lower metabolic rate and the chronology of gene expression and translational control suggest that developmental competence also plays a decisive role. In addition, maternal effect genes provide a defense potential until the mid-blastula transition. The transition between maternal effect genes and zygotic genes is a critical period for the embryo. The perivitelline fluids are an important trap for cations, but are also the major barrier to diffusion of gases and solutes. Acidic environmental pH interferes with acid-base and hydromineral balance but also increases the risk of aluminium and heavy metal intoxication. These risks are ameliorated somewhat by

  4. The physiology and toxicology of salmonid eggs and larvae in relation to water quality criteria

    Energy Technology Data Exchange (ETDEWEB)

    Finn, Roderick Nigel [Department of Biology, University of Bergen, Allegaten 41, N-5020 Bergen (Norway)]. E-mail: nigel.finn@bio.uib.no

    2007-03-30

    The purpose of this review is to collate physiological knowledge on salmonid eggs and larvae in relation to water quality criteria. Salmonid genera reviewed include Coregonus, Thymallus, Salvelinus, Salmo, and Oncorhynchus spp. When physiological data for salmonids are lacking, the zebrafish and medaka models are included. The primary focus is on the underlying mechanisms involved in the hydro-mineral, thermal, and respiratory biology with an extended section on the xenobiotic toxicology of the early stages. Past and present data reveal that the eggs of salmonids are among the largest shed by any broadcast spawning teleost. Once ovulated, the physicochemical properties of the ovarian fluid provide temporary protection from external perturbations and maintain the eggs in good physiological condition until spawning. Following fertilisation and during early development the major structures protecting the embryo from poor water quality are the vitelline membrane, the enveloping layer and the chorion. The vitelline membrane is one of the least permeable membranes known, while the semi-permeable chorion provides both physical and chemical defense against metals, pathogens, and xenobiotic chemicals. In part these structures explain the lower sensitivity of the eggs to chemical imbalance compared to the larvae, however the lower metabolic rate and the chronology of gene expression and translational control suggest that developmental competence also plays a decisive role. In addition, maternal effect genes provide a defense potential until the mid-blastula transition. The transition between maternal effect genes and zygotic genes is a critical period for the embryo. The perivitelline fluids are an important trap for cations, but are also the major barrier to diffusion of gases and solutes. Acidic environmental pH interferes with acid-base and hydromineral balance but also increases the risk of aluminium and heavy metal intoxication. These risks are ameliorated somewhat by

  5. Lack of grading agreement among international hemostasis external quality assessment programs

    OpenAIRE

    Olson, John D.; Jennings, Ian; Meijer, Piet; Bon, Chantal; Bonar, Roslyn; Favaloro, Emmanuel J.; Higgins, Russell A.; Keeney, Michael; Mammen, Joy; Marlar, Richard A.; Meley, Roland; Nair, Sukesh C.; Nichols, William L.; Raby, Anne; Reverter, Joan C.

    2018-01-01

    Laboratory quality programs rely on internal quality control and external quality assessment (EQA). EQA programs provide unknown specimens for the laboratory to test. The laboratory's result is compared with other (peer) laboratories performing the same test. EQA programs assign target values using a variety of methods statistical tools and performance assessment of ‘pass’ or ‘fail’ is made. EQA provider members of the international organization, external quality assurance in thrombosis and h...

  6. Use of sodium dodecyl sulfate pretreatment and 2-stage curing for improved quality of salted duck eggs.

    Science.gov (United States)

    Lian, Zixuan; Qiao, Longshan; Zhu, Guanghong; Deng, Yun; Qian, Bingjun; Yue, Jin; Zhao, Yanyun

    2014-03-01

    The effects of use of sodium dodecyl sulfate (SDS) pretreatment and 2-stage curing on the microbial, physicochemical, and microstructural qualities of salted duck eggs were studied. After pretreatment in 0.5% (w/v) SDS solution at room conditions for 15 min, no discolorations were observed and no microorganisms were detected on the egg shells. In the 2-stage curing process, 25% (w/v) and 30% (w/v) saline solutions were evaluated in the 1st step (Stage I, approximately 18 d), whereas 4% (w/v) saline solution was applied in the 2nd step (Stage II, approximately 15 d). Along with increased curing time, water content decreased and NaCl content increased in the egg yolks from approximately 0.40% to 0.86%, whereas the water content of egg albumen remained at approximately 85% during the 2-stage curing. More importantly, the NaCl content of albumen maintained at approximately 4.0% at Stage II curing. Yolk index as a sign of maturity for salted duck eggs reached 1 at the end of Stage I (18 d) and retained the same value during Stage II curing regardless of the NaCl concentration in the Stage I saline solution. Oil exudation in egg yolks increased as the time of curing increased. As seen from scanning electron microscopy, oil was released from yolk granules. This study indicated that SDS pretreatment is effective to reduce microbial load on the shells of fresh duck eggs and the 2-stage curing can improve physicochemical qualities of the salted duck eggs and shortened curing time to about 7 to 17 d as compared to the traditional 1-step curing method. Spoiled saline solution and uneven distribution of salt are the 2 major problems in producing salted duck eggs. Sodium dodecyl sulfate (SDS) pretreatment and 2-stage curing process have shown effective to solve these problems, respectively. The SDS pretreatment was able to remove microorganisms and soil from the surface of fresh egg shells, thus preventing the spoilage of the saline solution. The 2-stage curing process

  7. Effect of dietary inclusion of sugar syrup on production performance, egg quality and blood biochemical parameters in laying hens

    Directory of Open Access Journals (Sweden)

    Ahmed S. Hussein

    2018-03-01

    Full Text Available The effects of dietary inclusion of sugar syrup on quality of egg, cholesterol level, production performance, serum total protein and blood biochemical parameters were evaluated in laying hens. A total of 300 commercial Lohmann LSL hens (30 weeks of age were randomly distributed into 3 dietary treatments which consisted of a normal corn diet containing corn–soy and 2 diets containing 5% and 10% sugar syrup. Each treatment was replicated 5 times (n = 20. Egg production, feed intake, body weight and egg weight of laying hens fed different diets were recorded. The experiment lasted for 20 weeks. The Haugh unit scores of hens fed diets with sugar syrup were significantly increased (P < 0.05 compared with the control treatment. The sugar syrup had no significant effect on liver enzymes, total protein, blood glucose and creatinine in all treatments. The eggs laid by hens fed sugar syrup diets had lower cholesterol level (P < 0.05 compared with those laid by hens fed the control diet. Electrophoresis analysis showed that comparable electrophoretic patterns were noticed between serum proteins of treatment groups. From the results, it can be concluded that sugar syrup diets and corn diets have similar effects on feed intake, body weight, production of eggs and blood biochemical parameters in layer hens, which suggests sugar syrup can be used as an energy source for replacing part of corn in poultry layer diets. Keywords: Cholesterol, Sugar syrup diet, Egg, Egg quality, Protein

  8. On the relative effect of spawning asynchrony, sperm quantity and sperm quality on paternity under sperm competition in an external fertilizer

    Directory of Open Access Journals (Sweden)

    Torvald Blikra Egeland

    2015-07-01

    Full Text Available How much of a fitness benefit is obtained by dominant males of external fertilizers from releasing ejaculates in synchrony with female egg-release when engaging in sperm competition, and what is the most important sperm trait for paternity in these situations? The Arctic charr (Salvelinus alpinus is an external fertilizer experiencing intense male-male competition over reproductive opportunities including sperm competition. To compensate for their disadvantage the sneaker males, which often spawn out of synchrony with the female, produce more and faster sperm than the guarding males. We used controlled in vitro fertilization trials with experimentally produced dominant and subordinate, sneaker males to test what effect relative synchrony in gamete release, sperm quality (i.e., motility and velocity and sperm quantity have on a male’s fertilization success in pair-wise sperm competitions. When the sneaker males released ejaculates after the guarding male there was no overall difference in fertilization success. The quality (i.e., motility and velocity of a male’s sperm relative to that of the competing male was the best predictor of male fertilization success regardless of their mating tactic and spawning synchrony. The relative number of sperm cells also had an effect on fertilization success, but mainly when the dominant and sneaker male ejaculated synchronously. Our close imitation of natural sperm competition in charr shows that the sneaker males of external fertilizing species may fully compensate for their disadvantaged mating role by producing ejaculates of higher quality - an adjustment strangely not met by dominants.

  9. Effect of Supplementation Purslane (Portulaca oleracea as a Source of Alpha-Linolenic Acid on Production Performance and Physical Quality of Egg of Laying Hens

    Directory of Open Access Journals (Sweden)

    Lilik Kartikasari

    2015-09-01

    Full Text Available The aim of the study was to evaluate the effects of inclusion plant source of n 3 fat in the form of alpha-linolenic acid (ALA, 18:3n-3 on the diets of layers on production performance and physical quality of eggs. A total of  125 Hy-Line Brown hens (38 weeks old were placed at individual cages and assigned to five dietary treatments. The dietary treatments were supplemented with 0, 1.5, 3.0, 4.5 and 6.0% purslane meal. Laying hens were fed for five weeks following a seven day adaptation period. Water and feed were provided ad libitum. Feed intake (FI measured weekly and feed consumption ratio (FCR was calculated at the end of the trial. A total of 25 egg yolk samples of day 35 (n = 5 egg yolks for each treatment were collected to analyse physical quality of eggs. The data were analyzed using Analysis of Variance (ANOVA. Differences between treatment means were further analyzed using Duncan's New Multiple Range Test (DMRT. Results showed that the incorporation of plants rich in ALA did not modify FI, FCR, and egg production. Supplementation of purslane meal in the diets had no effect on physical quality of eggs, including egg weight, yolk weight, albumen index, yolk index and Haugh Unit (HU. The average of egg weight and yolk weight were 60,5 and 15.3 g, respectively. Diet containing purslane meal increased yolk colour. In conclusion, laying hens that fed diet supplemented with purslane meal rich in ALA improved yolk colour and did not change the production performance of the laying hens or the qualities of the eggs.

  10. Infections may select for filial cannibalism by impacting egg survival in interactions with water salinity and egg density.

    Science.gov (United States)

    Lehtonen, Topi K; Kvarnemo, Charlotta

    2015-07-01

    In aquatic environments, externally developing eggs are in constant contact with the surrounding water, highlighting the significance of water parameters and pathogens for egg survival. In this study we tested the impact of water salinity, egg density and infection potential of the environment on egg viability in the sand goby (Pomatoschistus minutus), a small fish that exhibits paternal egg care and has a marine origin, but which in the Baltic Sea lives in brackish water. To manipulate the infection potential of the environment, we added either a Saprolegnia infection vector into UV-filtered water or a fungicide into natural Baltic Sea water. Saprolegnia are widely spread water moulds that are a key cause of egg mortality in aquatic organisms in fresh- and brackish water. We found that increased water salinity indeed decreased the egg infection rate and had a positive effect on egg viability, while high egg density tended to have the opposite effect. However, the different factors influenced egg viability interactively, with a higher egg density having negative effects at low, but not in high, salinity. Thus, the challenges facing marine organisms adapting to lower salinity levels can be amplified by Saprolegnia infections that reduce egg survival in interaction with other environmental factors. Our results support the hypothesis that suppressing egg infections is an important aspect of parental care that can select for filial cannibalism, a common but poorly understood behaviour, especially in fish with parental care.

  11. Ensuring microbiological safety of commercial eggs by gamma irradiation

    International Nuclear Information System (INIS)

    Hammad, A.A; Swailam, H.M.; Abd El-Rahim, E.A.; Shallan, M.A; Aly, W.F.

    2009-01-01

    Egg shell sanitizing practices are necessary to improve microbiological safety of fresh eggs. In this work, fifteen fresh eggs samples of each (balady, white and brown eggs) were collected from different local supermarkets to evaluate their microbiological quality. The results indicated that balady eggs were higher in microbial population in comparison with the two other varieties. Salmonella spp. was detected in 9 (60%) samples of balady eggs. The effect of gamma irradiation at dose of 0,2,3 and 4 kGy on microbiological quality of balady eggs during storage at 4± 1 deg c for 35 days as well as on total cholesterol , ph, and total carotenoids contents were also investigated. The results showed that irradiation at 3 kGy reduced total aerobic bacterial counts. Whereas completely eliminated coliform bacteria, E.coli and salmonella spp. Total cholesterol and total carotenoids were decreased in yolk egg. irradiation dose of 3 kGy were efficient and sufficient for ensuring safety, maintain quality and extend shelf-life of balady eggs

  12. Effects of Ascorbic Acid Injection in Incubated Eggs Submitted to Heat Stress on Incubation Parameters and Chick Quality

    Directory of Open Access Journals (Sweden)

    S Sgavioli

    2015-06-01

    Full Text Available Dose-dependent positive effects on hatchability and hatchling weight have been attributed to ascorbic acid (AA when eggs were submitted or not to intermittent heat stress during incubation. Fertile breeder (Cobb(r eggs were used to determine if the pre-incubation injection of AA in ovo affects the incubation and hatchling quality of egg incubated under thermoneutral or intermittent heat stress conditions. Eggs were not injected or injected with 0, 2,4, or 6% AA/100µL water and incubated at continuous thermoneutral (37.5ºC or hot (39.0ºC temperature. Eggshell temperature (EST increased in the second half of the incubation period in all experimental groups. The EST of non-injected eggs and of those injected with water was higher when incubated at 39°C than at 37.5°C, but EST was not different among eggs injected with AA. Egg mass loss and eggshell conductance were higher in the eggs incubated at 39°C than at 37.5°C.Hatchability was lower in the eggs injected with AA. Liver and yolk sac weights were higher, whereas heart and liver weights were lower in hatchlings from eggs incubated at 39°C; however, hatchling weight was not affected by incubation temperature. The results showed that AA doses affected egg conductive heat loss and hatchability, and that they did not minimize the effects of high incubation temperature on liver and heart development.

  13. New technologies to enhance quality and safety of table eggs: ultra-violet treatment and modified atmosphere packaging

    Directory of Open Access Journals (Sweden)

    Frédérique Pasquali

    2014-12-01

    Full Text Available In the present study the effect of ultra-violet (UV treatment alone and in combination with 100% CO2 modified atmosphere packaging (MAP was evaluated both on the survival of naturally occurring bacteria, as well as on quality parameters of table eggs during 28 days of storage at 21°C. Table eggs were collected from the conveyor belt after the UV module, and placed on carton trays. A representative number of carton trays were packed in a high barrier multilayer pouch filled with 100% CO2. All eggs were stored at 21°C and analysed at 0, 1, 7, 14, 21 and 28 days of storage. Eggs not treated with UV and not packed were also included. On the eggshells total colony count, total coliforms and faecal coliforms counts, as well as the detection of Salmonella spp. were investigated. Moreover, chemical-functional parameters such as weight loss, albumen pH and Haugh Unit (HU were evaluated. The total colony count on UV treated table eggs was approximately 1 log10 CFU/g lower than untreated eggs (2.27 vs 3.29 log10 CFU/g. During storage, CO2 packed eggs maintained the initial values of HU, whereas the albumen pH decreased up to 1.5-2 points in comparison to unpacked eggs. The UV treatment was effective in reducing the total colony count on the surface of table eggs. MAP showed a great potential in maintaining/enhance the technological properties of egg constituents (higher foam stability of the albumen for meringue preparation without significantly impacting on the microbial load of table eggs.

  14. Performance and egg quality traits of egg-type chickens fed ...

    African Journals Online (AJOL)

    FCR), egg weight (EW) and haugh units (HU). All data were analyzed using descriptive statistics and analysis of variance. The determined CP of CSC was 35.11% and its gossypol content was 570g/ton. FCR ranged from 1.6 to 4.9, HDP from ...

  15. Total lipid in the broodstock diet did not affect fatty acid composition and quality of eggs of sea bass (Dicentrarchus labrax L.

    Directory of Open Access Journals (Sweden)

    José Mª Navas

    2001-03-01

    Full Text Available To determine whether an increase in the quantity of lipids ingested by sea bass (Dicentrarchus labrax broodstock could improve egg quality, three year-old sea bass were fed three different diets: a natural diet (Control group, and two artificial diets containing 10% or 17% of total lipids. In two consecutive reproductive seasons, the spawning results, the lipid classes and the fatty acid composition of the eggs were studied. No differences in the absolute content of lipids or in the percentage of different lipid classes were observed between the eggs from the three groups. Both experimental groups fed with the artificial diets produced eggs of poor quality, with low percentages of buoyancy and hatching. Those observed in the eggs from the Control Group were significantly higher (p> 0.01 than those of the experimental groups. The higher hatching rate of the eggs from the Control Group was associated with higher DHA:EPA and AA:EPA ratios. The data obtained showed that the fatty acid composition of the eggs was affected by the fatty acid composition of the diets but not by the total quantity of lipids administered to the broodstock.

  16. Variability and interaction of some egg physical and eggshell quality attributes during the entire laying hen cycle.

    Science.gov (United States)

    Sirri, F; Zampiga, M; Berardinelli, A; Meluzzi, A

    2018-05-01

    The aim of this study was to investigate the variability and relationships between some egg physical (egg weight, width, length, shape index, and surface area) and eggshell parameters (weight and percentage, thickness, breaking strength, and L*, a*, and b* values) during the entire laying hen cycle. A total of 8,000 eggs was collected every 5 wk, from 30 to 81 wk of hen age (10 samplings of 400 eggs/house), in 2 identical poultry houses equipped with enriched cages. For the statistical analysis, ANOVA, Bivariate Correlation, Principal Component Analysis (PCA), and Hierarchical Cluster Analysis were used. An increase of egg weight, length, and eggshell lightness (L*) associated with a reduction of eggshell percentage, breaking strength, and redness (a*) was observed as the hen aged (P 10% of eggshell breaking strength and a*. According to the PCA, the highest changes during the laying cycle are related to egg physical parameters (32%) and to eggshell breaking strength, percentage, and thickness (26%). The egg physical parameters appeared to be strongly correlated to each other, whereas a slight correlation between eggshell breaking strength and color attributes were evidenced (-0.231 and 0.289, respectively, for L* and a*; P quality attributes throughout the entire laying hen cycle.

  17. The effect of dry caper (capparis spinosa) fruit on egg production and quality characteristics of laying hens

    International Nuclear Information System (INIS)

    Yildirim, A.; Tahtali, Y.; Sen, M. I.; Duman, M.

    2014-01-01

    This study was conducted to determine the effect of increasing dietary inclusion of dry Caper (Capparis spinosa) fruit (DCF) on egg production and quality characteristics of laying hens between 20 and 32 weeks of age. Four groups of commercial hens (ATAK-S) were fed with diets containing 0, 5, 10 and 15 g DCF/kg. The results showed that final body weight, feed intake, shape index (SI) and morning hen - day egg yield were influenced by dietary supplementation of DCF (P 0.05) during the entire experiment. The lightness (L*) and redness (a*) values for egg shell color were similar (P>0.05) in the DCL supplemented groups as compared to the control group. The yellowness (b*), Hue angle (H), chroma (C*) and E* values were lower (P<0.05) in 15 g DFC/kg group when compared with the control. Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and total phenolics amount of DCF were 43.75+-0.680 mmol trolox/kg, 60.03+-3.710 mmol TEAC/kg and 3.16+-0.060 g gallic acid equivalents (GAE)/kg, respectively. Based on the results from the current study, the dietary supplementation with dry DCF had adverse effects on productivity performance traits and egg quality. (author)

  18. Reproductive broodstock performance and egg quality of wild-caught and first-generation domesticated Seriola rivoliana reared under same culture conditions

    Directory of Open Access Journals (Sweden)

    Marcos F Quiñones-Arreola

    2015-11-01

    Full Text Available Almaco jack, Seriola rivoliana as well as some related species is of great interest in marine fish aquaculture. However, there are few studies about their reproduction in captivity. In this research work, reproductive performance and egg quality in two groups of adult Seriola rivoliana, caught in the wild and domesticated-F1 analyzed and compared, reared under optimal maturation conditions in a commercial private Laboratory. A total of 28 wild adult (>5 kg were caught at La Paz Bay, Baja California Sur, Mexico, and 30 adult domesticated-F1 broodstock (>5 kg, were obtained from an original stock of 1,000 juveniles (3.5 g body weight produced at Kona Blue (Hawaii, USA sea farm. Fishes were transported to the Rancheros del Mar commercial private hatchery, where they were grown to adult size. Both groups were evaluated during eight months (May to December 2012 and compared in terms of reproduction performance (total number of spawning events, monthly spawning frequency, total number of eggs, total number of eggs per mL, and fertilization rate, egg biochemical composition (total proteins, total lipids, total carbohydrates, and triacylglycerides and egg diameter. Results indicated that wild caught broostock showed a better reproductive performance in terms of fertilization rate, total number of spawning, monthly spawning frequency and total number of eggs produced. However, biochemical composition and egg diameter did not show statistical differences (P < 0.05 between two groups. The reproductive performance of broodstock and quality of eggs analyzed in this study are important traits to improve the aquaculture management of this species.

  19. Effect of organic mineral supplementation on the egg quality of semi-heavy layers in their second cycle of lay

    Directory of Open Access Journals (Sweden)

    ESPB Saldanha

    2009-12-01

    Full Text Available This study was carried out to evaluate the effects of dietary trace mineral levels and sources on egg quality parameters of second-cycle semi-heavy layers. A number of 360 72-week-old layers were submitted to forced molting. Upon return of lay (83 weeks of age, birds were distributed according to a completely randomized experimental design of six treatments with six replicates of 10 birds each. The control treatment consisted of 0.10% dietary supplementation of trace minerals from inorganic sources, which was proportionally replaced by five levels (110, 100, 90, 80, 70% of an organic trace mineral supplement containing 30, 30, 40, 6, 0.61, and 0.3 g/kg product of Zn, Fe, Mn, Cu, I, and Se, respectively. All diets contained equal protein, energy, and amino acid levels. Every 28 days of the experimental period (112 days four eggs per replicate were collected for egg quality evaluation. The following parameters were evaluated: specific gravity, yolk, albumen and eggshell percentages, yolk index, Haugh units, and eggshell thickness and breaking strength. One sample per replicate, consisting of the pool of the yolks of three eggs collected at the end of each experimental period, was used to assess protein and mineral (Ca, P, Cu, Fe, Mn, and Zn contents. The results were submitted to ANOVA, and means to the test of Tukey at 5% significance level. The evaluated trace mineral levels and sources did not influence any of the studied egg quality parameters. It was concluded that reducing organic trace mineral supplementation in up to 70% relative to 100% inorganic trace mineral supplementation does not affect egg parameters and therefore, can be applied to the diet of semi-heavy layers in their second cycle of lay.

  20. Ten Years of External Quality Audit in Australia: Evaluating Its Effectiveness and Success

    Science.gov (United States)

    Shah, Mahsood

    2012-01-01

    External quality audits are now being used in universities across the world to improve quality assurance, accountability for quality education and transparency of public funding of higher education. Some countries such as Australia, New Zealand, United Kingdom, Sweden and Denmark have had external quality audits for more than a decade but there…

  1. Ensuring Microbiological Safety of Fresh Eggs by Using Gamma Irradiation

    International Nuclear Information System (INIS)

    Hammad, A. A.; Swailam, H.M.; Aly, W. F.

    2008-01-01

    Egg shell sanitizing practices are necessary to improve microbiological safety of fresh eggs. In this work, fifteen fresh eggs samples of each (Balady, White and Brown eggs) were collected from different local supermarkets to evaluate their microbiological quality. The results indicated that Balady eggs were higher in microbial population in comparison with the two other varieties. Salmonella spp. was detected in 9 (60%) samples of Balady eggs. The effect of gamma irradiation at dose of 0, 2, 3 and 4 kGy on microbiological quality of Balady eggs during storage at 4±1 degree C for 35 days was studied. The effects of irradiation and storage on total cholesterol, and total carotenoids contents in Balady eggs were also investigated. The results showed that irradiation at 3 kGy reduced total aerobic bacterial counts. Coliform bacteria, E. coli and Salmonella spp. were completely eliminated. Total cholesterol and total carotenoids were decreased in yolk egg as a result of irradiation. Treated Balady eggs with irradiation dose of 3 kGy were efficient and sufficient for ensuring safety, maintain quality and extend shelf-life.

  2. Effect of different levels of methionine, protein and tallow on the productive performance and egg quality of laying hens in the late-phase production

    Directory of Open Access Journals (Sweden)

    H Nassiri Moghaddam

    2012-06-01

    Full Text Available An experiment was conducted to determine the effects of different levels of methionine, protein and tallow on productive performance and egg quality of laying hens in the late phase of production. A completely randomized design with a 3×2×2 factorial arrangement, with three levels (0.34, 0.31, and 0.27% of methionine (MET, two levels (12.8 and 14.7% of protein (PRO and two levels (1 and 3% of tallow (TAL with constant level of linoleic acid (1.55 ± 0.02%, was used. A number of 144 Hi-Line W-36 layers from 70 to 76 wk of age was randomly distributed into 12 treatment groups with 4 replicates of 3 hens each. Egg production and egg weight were daily recorded and feed intake and egg quality traits were recorded every 2 wk. There was a significant interaction between PRO levels and TAL for egg weight. Low levels of TAL and PRO decreased egg weight throughout the experiment. High levels of MET and TAL with concomitant reduced PRO, increased eggshell thickness, and a significant interaction between levels of MET, PRO and TAL was observed during the experiment (70 to 76 wk. Low level of protein (12.8% significantly decreased albumen weight in the third 2-wk period. Yolk color increased when hens were fed low levels of PRO and TAL. Results of this experiment indicated that the simultaneous reduction of dietary PRO and MET in diets of Hi-Line W-36 laying hens in the late phase of production, reduced egg weight (P<0.05. Productive performance and egg quality were not affected by 12 and 20% reduction of PRO and MET, respectively. It seems that decreasing the levels of MET and PRO to lower than the recommended values can decrease egg weight without negative effects on productive performance and egg quality of laying hens in the late phase of production.

  3. Texture and quality properties of Chinese fresh egg noodles formulated with green seaweed (Monostroma nitidum) powder.

    Science.gov (United States)

    Chang, H C; Wu, L-C

    2008-10-01

    The objective of this study was to compare fresh Chinese noodles made with different levels of green seaweed. Green seaweed powder was incorporated in proportions of 4%, 6%, and 8% in noodles, which were made with or without additional eggs. Proximate compositions, cooking properties, textural intensities, and sensory qualities of noodles were assessed. The addition of seaweed powder increased the crude fiber contents of raw fresh noodles; the fiber contents were 0.100%+/- 0.015 to 0.449%+/- 0.013 for noodles made with eggs from 0% to 8% additional seaweed and 0.247%+/- 0.018 to 0.344%+/- 0.021 for those without eggs. Higher cooking yields were found in the noodles, due to water absorption during cooking by the fibers and polysaccharides in the seaweed. Significantly higher cooking yields (P seaweed powder; water uptake readings measured 2.39 +/- 0.38 and 2.43 +/- 0.25 g H(2)O/g noodle for samples made without and with eggs, respectively. Higher water absorption by the seaweed led to softer and spongier textural intensities in the noodles. Breaking energy of cooked fresh egg noodles were 28.94 +/- 3.42 to 6.43 +/- 1.01 N x mm for 8% to 0% additional seaweed, and the intensities decreased as the amount of seaweed increased; the same pattern was observed in noodles without eggs, where readings were 8.66 +/- 1.02 to 3.49 +/- 0.25 N x mm. Capacities of extensibility measured 61.81 +/- 2.04 to 30.74 +/- 0.90 mm for fresh egg noodles with additional seaweed powder from 0% to 8%, and 47.46 +/- 2.41 to 28.36 +/- 2.25 mm for cooked fresh noodles without eggs. The results from Pearson's correlation analysis indicated that textural parameters were influenced not only by additional eggs and seaweed powder, but also by cooking properties.

  4. Influence of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzyme activities, and intestinal barrier function of laying hens.

    Science.gov (United States)

    Lei, K; Li, Y L; Yu, D Y; Rajput, I R; Li, W F

    2013-09-01

    This experiment was conducted to evaluate the effects of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzyme activities, and intestinal barrier function of laying hens. Hy-Line Variety W-36 hens (n = 540; 28 wk of age) were randomized into 6 groups, each group with 6 replications (n = 15). The control group received the basal diet formulated with maize and soybean meal. The treatment groups received the same basal diets supplemented with 0.01, 0.02, 0.03, 0.06, and 0.09% Bacillus licheniformis powder (2 × 10(10) cfu/g) for an 8-wk trial. The results showed that dietary supplementation with 0.01 and 0.03% B. licheniformis significantly increased egg production and egg mass. However, no significant differences were observed in egg weight, feed consumption, and feed conversion efficiency among the 6 groups. Supplementation with different levels of B. licheniformis was found to be effective in improvement of egg quality by increasing egg shell thickness and strength. Compared with control, d-lactate content, diamine oxidase activity, and adrenocorticotropic hormone level in serum decreased significantly, and the level of estradiol and follicle-stimulating hormone increased significantly in plasma of all the experimental groups. Dietary supplementation with B. licheniformis increased the intestinal villus height and reduced the crypt depth. In conclusion, dietary inclusion of B. licheniformis could improve laying performance and egg quality significantly in a dose-dependent manner by decreasing the stress response, upregulating the growth hormone, and improving intestinal health.

  5. Calcium and Egg Activation in Drosophila

    Science.gov (United States)

    Sartain, Caroline V.; Wolfner, Mariana F.

    2012-01-01

    Summary In many animals, a rise in intracellular calcium levels is the trigger for egg activation, the process by which an arrested mature oocyte transitions to prepare for embryogenesis. In nearly all animals studied to date, this calcium rise, and thus egg activation, is triggered by the fertilizing sperm. However in the insects that have been examined, fertilization is not necessary to activate their oocytes. Rather, these insects’ eggs activate as they transit through the female’s reproductive tract, regardless of male contribution. Recent studies in Drosophila have shown that egg activation nevertheless requires calcium and that the downstream events and molecules of egg activation are also conserved, despite the difference in initial trigger. Genetic studies have uncovered essential roles for the calcium-dependent enzyme calcineurin and its regulator calcipressin, and have hinted at roles for calmodulin, in Drosophila egg activation. Physiological and in vitro studies have led to a model in which mechanical forces that impact the Drosophila oocyte as it moves through the reproductive tract triggers the influx of calcium from the external environment, thereby initiating egg activation. Future research will aim to test this model, as well as to determine the spatiotemporal dynamics of cytoplasmic calcium flux and mode of signal propagation in this unique system. PMID:23218670

  6. Effect of gamma irradiation on hygienic quality and chemical characteristics of dehydrated ostrich eggs

    International Nuclear Information System (INIS)

    Shahin, A.A.M.; Swailam, H.M.; Zeid, A.A.A.

    2006-01-01

    Role of gamma irradiation on improving hygienic quality of dehydrated Ostrich eggs during cold storage was studied. Irradiation with dose level 6 kGy proved to be quite tool to keep quality of ostrich egg components since it decreased their bacterial counts less than 100 cells/g either after irradiation process or during cold storage for 6 months. Total coliform, E. coli and Staphylococcus sp. were almost eliminated. Twenty one fungi species belonging to eight genera were isolated from the un-treated samples. Most species could not stand cold storage and only five species (Aternaria clamydospora, Aspergillus niger, Asp. oryzae, Cladosporium cladosporioides & Fusarium oxysporum) were detected after 6 months of storage at 5 degree C. Also, five species could endure irradiation with dose level 6 kGy (Al. alternata, Al. clamydospora, Cl. cladosporioides, herbarum & F. oxysporum) and only two species of them could bear cold storage for 6 months (Al. clamydosp ora & Cl. clodosporioides). The effect of cold storage (5 degree C) on total amino acids either essential or non essential was relatively higher than that occurred by irradiating dried albumen ostrich eggs with 6 kGy. Total amino acids decreased from 272.5 mg/g to 241 mg/g after 6 months of cold storage, while they decreased to 256.2 mg/g after the irradiation process. The same trend was observed in case of dried whole yolk or defatted yolk. The effect of gamma rays and cold storage on the relative percentages of fatty acids composition of dried ostrich eggs yolk was different from each other. After exposing the dried yolk to 6 kGy, the percentages of myristic and palmitic acids increased, while palmitoleic and stearic acids decreased. Mean-while after 6 months of cold storage the percentages of palmitoleic and linoleic acids increased, while stearic and oleic acids decreased

  7. Effects of rearing systems on laying performance, egg quality, and serum biochemistry of Xianju chickens in summer.

    Science.gov (United States)

    Dong, X Y; Yin, Z Z; Ma, Y Z; Cao, H Y; Dong, D J

    2017-09-01

    The objective of this study was to compare the laying performance, egg quality, and serum biochemistry of hens maintained in conventional cage rearing system (CRS), flat net-rearing system (NRS), and free range system (FRS) under summer conditions. Indigenous Xianju chickens (n = 540) were randomly allocated into cages or pens of rearing system groups, within each system there were 5 replicates with 36 hens in each replicate. The experiment lasted between 21 and 29 wk of age. Hen-day egg production (P = 0.00) and egg mass (P = 0.00) were higher in the CRS but were similar in the NRS and FRS. Lowest egg weight (P = 0.02), yolk weight (P = 0.00) and yolk ratio (P = 0.01), and feed intake (P = 0.01) were observed from the FRS, whereas lowest feed conversion ratio (FCR) was recorded from the CRS (P = 0.01). Rearing systems had negligible effect on egg quality. Serum Ca (P = 0.04) and total protein (P = 0.03) levels were found to be higher in the CRS but were lower in the FRS. Serum levels of glucose (P = 0.01), cholesterol (P = 0.00), and triglyceride (P = 0.00) in the CRS increased compared with the NRS and FRS groups, whereas serum levels of high density lipoprotein cholesterol (HDL-C; P = 0.01) in the CRS decreased. It can be concluded that under summer conditions, Xianju chickens from CRS had an advantage in terms of productivity parameters, but exhibited higher levels of serum lipids and glucose. © 2017 Poultry Science Association Inc.

  8. Impact of egg disinfection of hatching eggs on the eggshell microbiome and bacterial load

    DEFF Research Database (Denmark)

    Olsen, R.; Kudirkiene, E.; Thofner, I.

    2017-01-01

    Disinfection of hatching eggs is essential to ensure high quality production of broilers. Different protocols are followed in different hatcheries; however, only limited scientific evidence on how the disinfection procedures impact the microbiome is available. The aim of the present study...... was to characterize the microbiome and aerobic bacterial load of hatching eggs before disinfection and during the subsequent disinfection steps. The study included a group of visibly clean and a group of visibly dirty eggs. For dirty eggs, an initial wash in chlorine was performed, hereafter all eggs were submitted...... to two times fumigation and finally spray disinfection. The eggshell microbiome was characterized by sequencing of the total amount of 16S rRNA extracted from each sample, consisting of shell surface swabs of five eggs from the same group. In addition, the number of colony forming units (cfu) under...

  9. Performance and egg quality of aged laying hens fed diets ...

    African Journals Online (AJOL)

    p2492989

    receiving OSM produced more eggs and egg mass along with considerably greater feed intake than those on all the other .... initiation of the second egg production period, were housed in cages located in a three-deck battery-type cage block. .... Diets (in mash form) and water were provided for ad libitum consumption.

  10. Ground annatto seeds (Bixa orellana L. in sorghum-based commercial layer diets and their effects on performance, egg quality, and yolk pigmentation

    Directory of Open Access Journals (Sweden)

    EA Garcia

    2010-12-01

    Full Text Available Consumer demands for healthy foods have stimulated the research on the use of natural products in animal nutrition. Annatto can be used a pigmentation source to improve yolk color of commercial eggs. This study aimed at evaluating the effects of the inclusion of ground annatto seeds on the performance and egg quality of layers fed sorghum-based diets. A total of 336 40-w-old hens were distributed according to randomized blocks into seven treatments with six replicates of eight birds each. The following treatments were applied: T1- egg production feed based on corn and soybean meal; T2- egg production feed based on sorghum and soybean meal; T3, T4, T5, T6, and T7- egg production feed based on sorghum supplemented with 0.5, 1.0, 1.5, 2.0, and 2.5% ground annatto seeds, respectively. As to performance parameters, only egg production was influenced, with hens fed the corn-based diet producing more eggs than those fed the sorghum-based diet with inclusion of 1.5 and 2.5% de annatto. The only egg quality parameter affected by treatments was the color of yolk, which pigmentation increased with increasing levels of ground annatto seeds. It is concluded that ground annatto seeds can be supplemented in sorghum-based production feeds for layers, and that the inclusion level of 0.89% is sufficient to promote the same yolk pigmentation as that obtained with corn-based diets.

  11. Maternal effects mediated by egg quality in the Yellow-legged Gull Larus michahellis in relation to laying order and embryo sex

    Directory of Open Access Journals (Sweden)

    Caprioli Manuela

    2011-10-01

    Full Text Available Abstract Background Maternal effects mediated by egg size and quality may profoundly affect offspring development and performance, and mothers may adjust egg traits according to environmental or social influences. In avian species, context-dependency of maternal effects may result in variation in egg composition, as well as in differential patterns of covariation among selected egg components, according to, for example, position in the laying sequence or offspring sex. We investigated variation in major classes of egg yolk components (carotenoids, vitamins and steroid hormones in relation to egg size, position in the laying sequence and embryo sex in clutches of the Yellow-legged Gull (Larus michahellis. We also investigated their covariation, to highlight mutual adjustments, maternal constraints or trade-offs in egg allocation. Results Laying sequence-specific patterns of allocation emerged: concentration of carotenoids and vitamin E decreased, while concentrations of androgens increased. Vitamin A, estradiol and corticosterone did not show any change. There was no evidence of sex-specific allocation or covariation of yolk components. Concentrations of carotenoids and vitamins were positively correlated. Egg mass decreased along the laying sequence, and this decrease was negatively correlated with the mean concentrations of carotenoids in clutches, suggesting that nutritionally constrained females lay low quality clutches in terms of carotenoid content. Finally, clutches with smaller decline in antioxidants between first- and last-laid eggs had a larger increase in yolk corticosterone, suggesting that a smaller antioxidant depletion along the laying sequence may entail a cost for laying females in terms of increased stress levels. Conclusions Since some of the analyzed yolk components (e.g. testosterone and lutein are known to exert sex-specific phenotypic effects on the progeny in this species, the lack of sex-specific egg allocation by

  12. Laboratory diagnosis of the rare anaemias: external quality assessment benefits patient care

    OpenAIRE

    Barbara De La Salle; Andrea Mosca; Renata Paleari; Vasileios Rapanakis; Keith Hyde

    2013-01-01

    Since its introduction in the 1960s, external quality assessment has developed to become an essential component of the quality management system of the diagnostic laboratory. External quality assessment provides a long term, retrospective view of laboratory performance, demonstrating the competence of the laboratory to others. The ENERCA project (the European Network for Rare and Congenital Anaemias) has established a list of core laboratory tests that are used in the diagnosis of rare and co...

  13. Effect of dietary supplementation with Morinda citrifolia on productivity and egg quality of laying hens

    Directory of Open Access Journals (Sweden)

    Dairon Más-Toro

    2015-07-01

    Full Text Available In order to evaluate the dietary supplementation of powdered leaves of Morinda citrifolia on productivity and egg quality of laying hens, a total of 160 White Leghorn birds (Hybrid L-33 of 27 weeks of age were allotted during 70 days, according to completely randomized design. Dietary treatments consisted of a control diet fed without or with 0.5, 1.0 and 1.5% of powdered leaves of M. citrifolia. Supplementation of 1.0 and 1.5% of M. citrifolia powder increased the egg weight (P0.05 among treatments. Also, supplementation of 0.5 and 1.0% of M. citrifolia increased the shell thickness and the yolk color was pigmented by this medicinal plant. It recommended the dietary supplementation of 1.0% of powdered leaves of M. citrifolia on laying hen diets to improve the egg weight, shell thickness and yolk color.

  14. Higher Storage Temperature Causes Greater Salmonella enterica Serovar Typhimurium Internal Penetration of Artificially Contaminated, Commercially Available, Washed Free Range Eggs.

    Science.gov (United States)

    Whiley, Alice; Fallowfield, Howard; Ross, Kirstin; McEvoy, Vanessa; Whiley, Harriet

    2016-07-01

    Foodborne salmonellosis is a major public health concern, with contaminated eggs identified as a significant source of infection. In Australia, the most prevalent cause of salmonellosis from eggs is Salmonella enterica subsp. enterica serovar Typhimurium. This study explored the effect of temperature after 1, 7, 14, 21, and 28 days of storage on commercially available washed free range eggs, artificially contaminated with Salmonella Typhimurium on the external surface. At each time point, the external surface of the egg, the crushed eggshell, and the internal egg yolk and albumen were analyzed for Salmonella. After 28 days of storage, 25% of eggs stored at 4°C, 50% of eggs stored at 14°C, and 100% of eggs stored at 23 and 35°C were internally contaminated with Salmonella. After 1 day of storage, more than 50% of all eggs had Salmonella present in the crushed shell after the external surface had been disinfected with ethanol. This is the first study to demonstrate that refrigeration reduced the potential for Salmonella Typhimurium to penetrate the eggshell membrane and internally contaminate table eggs commercially available in Australia. It also suggests that the processes of cracking eggs may be a source of cross-contamination within the kitchen.

  15. Effects of Octacosanol Extracted from Rice Bran on the Laying Performance, Egg Quality and Blood Metabolites of Laying Hens.

    Science.gov (United States)

    Peng, Kai; Long, Lei; Wang, Yuxi; Wang, Shunxi

    2016-10-01

    A 42-d study with 384 Hy-line brown laying hens was conducted to assess the effects of dietary octacosanol supplementation on laying performance, egg quality and blood metabolites of laying hens. Hens were randomly allocated into 4 dietary groups of 8 cages each, which were fed basal diet supplemented with 0 (Control), 9 (OCT9), 18 (OCT18), and 27 (OCT27) mg/kg diet of octacosanol isolated from rice bran, respectively. The experiment was conducted in an environmental controlled house and hens were fed twice daily for ad libitum intake. Laying performance was determined over the 42-d period, and egg quality as well as blood metabolites were estimated on d 21 and d 42. Diets in OCT18 and OCT27 increased (pfeed conversion rate and levels of total cholesterol, triglyceride and low density lipoprotein cholesterol in the serum as compared to those of Control. Feed intake, yolk color, yolk diameter, eggshell thickness and high density lipoprotein cholesterol were similar (p>0.05) among treatments. Results demonstrate that supplementing 18 to 27 mg/kg diet of rice bran octacosanol can improve laying rate and egg quality and reduce blood lipid of laying hens.

  16. External quality audit programmes for radiotherapy dosimetry and equipment

    International Nuclear Information System (INIS)

    Thwaites, D.I.

    1997-01-01

    It is widely accepted that individual radiotherapy centres should have in place a comprehensive quality assurance programme on all the necessary steps for the delivery of safe accurate treatment. As regards the performance of radiotherapy equipment and dosimetry, the most widely used process of external checking has been dosimetry intercomparison, comparing independently measured doses to locally stated doses in a variety of conditions. These have been at a number of different levels: from basic beam calibration; up to and including exercises employing anatomic or pseudo-anatomic phantoms and incorporating tests of treatment planning equipment and procedures. Some of these have been one-off exercises, whilst others are continuing, or have given rise to on-going quality audit programmes on a national (or wider) basis. A number of these have evolved, or are evolving, into audits which include external checking of the achievement of standards in performance of treatment equipment, as well as in the dosimetry in each institution involved. The principles and methodologies of the various types of external checking programmes for treatment equipment and dosimetry are reviewed, covering the experimental approaches and the tolerances applied. What is included in a given programme will, of necessity, depend on the resources available and the purpose of the exercise. Methods and tolerances must be matched to endpoint. Tolerance levels must take into account the experimental uncertainties of the measurement methods employed. Finally, external audit can only be used to complement, and in conjunction with, institutional quality assurance programmes and not as a substitute for them

  17. Evaluation of the alpha-amylase activity as an indicator of pasteurization efficiency and microbiological quality of liquid whole eggs.

    Science.gov (United States)

    Silva, Guilherme Resende da; Menezes, Liliane Denize Miranda; Lanza, Isabela Pereira; Oliveira, Daniela Duarte de; Silva, Carla Aparecida; Klein, Roger Wilker Tavares; Assis, Débora Cristina Sampaio de; Cançado, Silvana de Vasconcelos

    2017-09-01

    In order to evaluate the efficiency of the pasteurization process in liquid whole eggs, an UV/visible spectrophotometric method was developed and validated for the assessment of alpha-amylase activity. Samples were collected from 30 lots of raw eggs (n = 30) and divided into three groups: one was reserved for analysis of the raw eggs, the second group was pasteurized at 61.1°C for 3.5 minutes (n = 30), and the third group was pasteurized at 64.4°C for 2.5 minutes (n = 30). In addition to assessing alpha-amylase activity, the microbiological quality of the samples was also evaluated by counting total and thermotolerant coliforms, mesophilic aerobic microorganisms, Staphylococcus spp., and Salmonella spp. The validated spectrophotometric method demonstrated linearity, with a coefficient of determination (R2) greater than 0.99, limits of detection (LOD) and quantification (LOQ) of 0.48 mg kg-1 and 1.16 mg kg-1, respectively, and acceptable precision and accuracy with relative standard deviation (RSD) values of less than 10% and recovery rates between 98.81% and 105.40%. The results for alpha-amylase activity in the raw egg samples showed high enzyme activity due to near-complete hydrolysis of the starch, while in the eggs pasteurized at 61.1°C, partial inactivation of the enzyme was observed. In the samples of whole eggs pasteurized at 64.4°C, starch hydrolysis did not occur due to enzyme inactivation. The results of the microbiological analyses showed a decrease (P pasteurized egg samples according to the two binomials under investigation, compared to the raw egg samples, which showed high rates of contamination (P pasteurization, only one sample (3.33%) was positive for Salmonella spp., indicating failure in the pasteurization process, which was confirmed by the alpha-amylase test. It was concluded that the validated methodology for testing alpha-amylase activity is adequate for assessing the efficiency of the pasteurization process, and that the time

  18. Irradiation of shell egg on the physicochemical and functional properties of liquid egg white.

    Science.gov (United States)

    Min, B; Nam, K C; Jo, C; Ahn, D U

    2012-10-01

    This study was aimed at determining the effect of irradiation of shell eggs on the physiochemical and functional properties of liquid egg white during storage. Color and textural parameters of irradiated liquid egg white after cooking were also determined. Shell eggs were irradiated at 0, 2.5, 5, or 10 kGy using a linear accelerator. Egg white was separated from yolk and stored in at 4°C up to 14 d. Viscosity, pH, turbidity, foaming properties, color, and volatile profile of liquid egg white, and color and texture properties of cooked egg white were determined at 0, 7, and 14 d of storage. Irradiation increased the turbidity but decreased viscosity of liquid egg white. Foaming capacity and foam stability were not affected by irradiation at lower dose (2.5 kGy), but were deteriorated at higher doses (≥5.0 kGy) of irradiation. Sulfur-containing volatiles were generated by irradiation and their amounts increased as the irradiation dose increased. However, the sulfur volatiles disappeared during storage under aerobic conditions. Lightness (L* value) and yellowness (b* value) decreased, but greenness (-a* value) increased in cooked egg white in irradiation dose-dependent manners. All textural parameters (hardness, adhesiveness, cohesiveness, chewiness, and resilience) of cooked egg white increased as the irradiation dose increased, but those changes were marginal. Our results indicated that irradiation of shell egg at lower doses (up to 2.5 kGy) had little negative impact on the physiochemical and functional properties of liquid egg white, but can improve the efficiency of egg processing due to its viscosity-lowering effect. Therefore, irradiation of shell eggs at the lower doses has high potential to be used by the egg processing industry to improve the safety of liquid egg without compromising its quality.

  19. Sensory quality of mayonnaise formulated with eggs produced by laying hens fed diet enriched with purslane meal (Portulaca oleracea)

    Science.gov (United States)

    Kartikasari, L. R.; Hertanto, B. S.; Nuhriawangsa, A. M. P.

    2018-01-01

    The objective of the study was to assess the sensory quality of mayonnaise formulated by egg yolks produced by hens fed purslane meal as a source of omega-3 (n-3) fats, alpha-linolenic acid. Eggs used were produced by Hy-Line Brown hens (38 weeks old) fed five diets suplemented with 0, 2, 4, 6 and 8% purslane meal. A total of 40 eggs of day 29 (n = 8 eggs for each treatment) were collected to formulate mayonnaise. The ingredient composition of mayonnaise was 14% egg yolks, 9% vinegar, 74% corn oil, 1% salt, 1% sugar, and 1% mustard. The parameters included colour, emulsion stability, texture, homogeneity, aroma, taste and flavour of mayonnaise. The findings of sensory evaluation by quantitative descriptive analysis showed that the use of egg yolk from hens fed dietary purslane meal up to 8% resulted in a significant effect on the yellow color of mayonnaise, but did not significantly affect emulsion stability, texture, homogeneity, aroma, taste, and flavour. Based on consumer preference, consumers did not detect any differences in the sensory characteristics between mayonnaise prepared from control eggs and those formulated with eggs produced from dietary treatment. Apparently, there was an increase in colour liking by inclusion of purslane meal. In conclusion, eggs from laying hens fed diets supplemented with purslane meal improved the colour intensity of mayonnaise. Mayonnaise formulation using eggs from diets added with Portulaca oleraceae meal up to a level of 8% can be applied without influencing mayonnaise sensory characteristics and consumer acceptance.

  20. 77 FR 21086 - Patents External Quality Survey (formerly Customer Panel Quality Survey)

    Science.gov (United States)

    2012-04-09

    ... corporations (including those with 500+ employees), small and medium-size businesses, and universities and... DEPARTMENT OF COMMERCE United States Patent and Trademark Office Patents External Quality Survey... their name, address, or Social Security Number. In order to access and complete the online survey...

  1. Dietary inclusion of raw faba bean instead of soybean meal and enzyme supplementation in laying hens: Effect on performance and egg quality

    Directory of Open Access Journals (Sweden)

    M.E. Abd El-Hack

    2017-02-01

    Full Text Available An experiment was conducted with 160 Hisex Brown laying hens to evaluate the effect of different inclusion levels of faba bean (FB and enzyme supplementation on productive performance and egg quality parameters. The experimental diets consisted of five levels of FB: 0% (control, 25%, 50%, 75% and 100%, substituting soybean meal (SBM, and two levels of enzyme supplementation (0 or 250 mg/kg. Each dietary treatment was assigned to four replicate groups and the experiment lasted 22 weeks. A positive relationship (P  0.05. The main effect of FB levels replacing for SBM affected (P < 0.05 yolk and shell percentages, yolk index, yolk to albumen ratio, shell thickness and egg shape index. It can be concluded that FB and enzyme supplementation could be included in hens diet at less than 50% instead of SBM to support egg productive performance, however higher raw FB levels negatively affected egg production indices and quality.

  2. Migration and income transfers in the presence of labor quality externalities

    NARCIS (Netherlands)

    Huizinga, H.P.

    1994-01-01

    In this paper, a worker's productivity is assumed to depend on his own quality and on the average quality of other workers in the same country. The external effects associated with worker quality give rise to increasing returns to average worker quality. As a result, free migration generally reduces

  3. Eggs: good or bad?

    Science.gov (United States)

    Griffin, Bruce A

    2016-08-01

    Eggs have one of the lowest energy to nutrient density ratios of any food, and contain a quality of protein that is superior to beef steak and similar to dairy. From a nutritional perspective, this must qualify eggs as 'good'. The greater burden of proof has been to establish that eggs are not 'bad', by increasing awareness of the difference between dietary and blood cholesterol, and accumulating sufficient evidence to exonerate eggs from their associations with CVD and diabetes. After 60 years of research, a general consensus has now been reached that dietary cholesterol, chiefly from eggs, exerts a relatively small effect on serum LDL-cholesterol and CVD risk, in comparison with other diet and lifestyle factors. While dietary guidelines have been revised worldwide to reflect this view, associations between egg intake and the incidence of diabetes, and increased CVD risk in diabetes, prevail. These associations may be explained, in part, by residual confounding produced by other dietary components. The strength of evidence that links egg intake to increased CVD risk in diabetes is also complicated by variation in the response of serum LDL-cholesterol to eggs and dietary cholesterol in types 1 and 2 diabetes. On balance, the answer to the question as to whether eggs are 'bad', is probably 'no', but we do need to gain a better understanding of the effects of dietary cholesterol and its association with CVD risk in diabetes.

  4. Payment for egg donation and surrogacy.

    Science.gov (United States)

    Steinbock, Bonnie

    2004-09-01

    This article examines the ethics of egg donation. It begins by looking at objections to noncommercial gamete donation, and then takes up criticism of commercial egg donation. After discussing arguments based on concern for offspring, inequality, commodification, exploitation of donors, and threats to the family, I conclude that some payment to donors is ethically acceptable. Donors should not be paid for their eggs, but rather they should be compensated for the burdens of egg retrieval. Making the distinction between compensation for burdens and payment for a product has the advantages of limiting payment, not distinguishing between donors on the basis of their traits, and ensuring that donors are paid regardless of the number or quality of eggs retrieved.

  5. INFLUENCE OF ASCORBIC ACID ON EGG QUALITY INFLUÊNCIA DO ÁCIDO ASCÓRBICO NA QUALIDADE DO OVO

    Directory of Open Access Journals (Sweden)

    Pedro Alves de SOUZA

    2001-12-01

    Full Text Available The egg quality may be influenced by some nutrients added to laying hens diets and among these nutrients, the ascorbic acid is included. According to some researches, this acid, besides of improving the quality, it can increase the egg production and weight. In addition, it reduces the quantity of cracked eggs and food intake. In order to contribute with more information, this experiment was developed. Concentrations of 100; 150 and 200mg/kg of ascorbic acid were added to rations of laying hens during 60 days, with the objective of determining the influence of ascorbic acid on the quality of eggs kept under room temperature conditions (26.7 ± 2.2°C and 60 ± 5% moisture for a period of 28 days. A total of 1440 eggs were used, being 720 of white shell and 720 of brown shell. Egg quality was determined by Haugh unit and yolk index, every 7 days. The obtained results allowed to conclude that ascorbic acid, independent on the level of supplementation, was not enough to decrease.A qualidade dos ovos pode ser influenciada por alguns nutrientes adicionados à dieta das aves e entre esses nutrientes se inclui o ácido ascórbico, que segundo alguns pesquisadores, além de melhorar a qualidade, pode aumentar a produção e o peso dos ovos e diminuir a quantidade de ovos trincados e o consumo de ração. Com a finalidade de tentar contribuir com mais algumas informações, desenvolveu esse experimento com o objetivo de avaliar a influência do ácido ascórbico sobre a qualidade de ovos brancos e marrons mantidos sob condições de ambiente (26,7 ± 2,2°C e 60 ± 5%UR, por um período de 28 dias. Foram utilizados 1440 ovos, sendo 720 de casca branca e 720 de casca marrom. Foram adicionadas à ração de aves, durante 60 dias, concentrações de 100, 150 e 200mg/kg de ácido ascórbico. A qualidade dos ovos foi determinada de 7 em 7 dias, avaliando-se a unidade Haugh e o índice gema. Os dados obtidos permitiram concluir que a suplementação com o

  6. Fermented feed for laying hens: effects on egg production, egg quality, plumage condition and composition and activity of the intestinal microflora.

    Science.gov (United States)

    Engberg, R M; Hammershøj, M; Johansen, N F; Abousekken, M S; Steenfeldt, S; Jensen, B B

    2009-03-01

    1. An experiment with a total of 480 hens (Babcock) was carried out from 16 to 38 weeks of age to evaluate the suitability of wet fermented feed (feed water ratio, 1:1.2-1:1.4) for layers, taking aspects of nutrition and gastrointestinal health into consideration. The production performance, egg shell quality, plumage condition, litter dry matter (DM) content, as well as the composition and activity of the intestinal microbial flora were analysed. 2. Fermented feed was characterised by a high concentration of lactic acid (160-250 mmol/kg feed) and a moderate level of acetic acid (20-30 mmol/kg feed), high numbers of lactic acid bacteria (log 9-10 CFU/g feed) and a pH of approximately 4.5. Feed fermentation reduced the concentration of dietary sugar from 32.1 to 7.3 g/kg DM and the phytate bound phosphorus from 2.7 to 1.9 g/kg DM. 3. Fermented feed seemed to loose attractiveness for the birds quite rapidly, resulting in a more aggressive behaviour and a poorer plumage condition than in birds given dry feed. The use of fermented feed reduced the litter DM content. 4. During the experimental period, the body weight gain of hens receiving fermented feed was 80 g higher than of hens fed the dry mash. Presumably because of an extended adaptation time to the feed, the onset of lay occurred later when hens were fed on fermented feed, resulting in non-significantly reduced total egg production (75 vs. 82%). 5. There was no significant difference between groups with respect to the total egg mass production (g/d/hen, 42 and 45 for fermented feed and dry mash, respectively). Throughout the experimental period, the feed DM intake of hens fed with fermented feed was lower than that of hens receiving the dry mash (110 vs. 125 g). From week 26 to 37, fermented feed improved the feed conversion as compared with the dry mash (g feed DM/g egg mass, 2.28 vs. 2.53). 6. The use of fermented feed increased egg weight in the period from 34 to 37 weeks (61.4 vs. 60.0) and increased shell

  7. The Market of Eggs, Consumption, and Consumer Behavior

    OpenAIRE

    Mustafa Pllana; Iliriana Miftari; Njazi Bytyqi; Vetim Hyseni

    2015-01-01

    Eggs are a traditional food with a high consumption per head even in economically developed countries with high living standard. Due to “cholesterol phobia†efforts were made to decrease the cholesterol content in the egg, but without success. Thus began research to improve the nuntrional quality of eggs, reposition them in safety and healthy food products. Today in markets there are organic eggs, vegetarian, nutrient enhaced eggs (omega-3, vitamin enhanced), for what is in order to expect...

  8. Digestibility, productive performance, and egg quality of laying hens as affected by dried cassava pulp replacement with corn and enzyme supplementation.

    Science.gov (United States)

    Khempaka, Sutisa; Maliwan, Prapot; Okrathok, Supattra; Molee, Wittawat

    2018-02-24

    Two experiments were conducted to investigate the potential use of dried cassava pulp (DCP) supplemented with enzymes as an alternative feed ingredient in laying hen diets. In experiment 1, 45 laying hens (Isa Brown) aged 45 weeks were placed in individual cages to measure nutrient digestibility for 10 days. Nine dietary treatments were control and DCP as a replacement for corn at 20, 25, 30, and 35% supplemented with mixed enzymes (cellulase, glucanase, and xylanase) at 0.10 and 0.15%. Results showed that the use of DCP at 20-35% added with mixed enzymes had no negative effects on dry matter digestibility, while organic matter digestibility and nitrogen retention decreased with increased DCP up to 30-35% in diets. Both enzyme levels (0.10 and 0.15%) showed similar results on nutrient digestibility and retention. In experiment 2, a total of 336 laying hens aged 32 weeks were randomly allocated to seven dietary treatments (control and DCP-substituted diets at 20, 25, and 30%) supplemented with mixed enzymes (0.10 and 0.15%). Diets incorporated with 20-30% of DCP and supplemented with mixed enzymes at both levels had no significant effects on egg production, egg weight, feed intake, egg mass, feed conversion ratio, protein efficiency ratio, or egg quality, except for egg yolk color being decreased with an increase of DCP in diets (P digestibility, productive performance, or egg quality.

  9. Reciprocal crosses between Alabio and Mojosari ducks : early egg production

    Directory of Open Access Journals (Sweden)

    L.H Prasetyo

    2000-12-01

    Full Text Available alternative to increasing productivity and production efficiency. Crossbreeding has been used widely for increasing productivity, and in this experiment Alabio and Mojosari ducks were crossed reciprocally in order to evaluate their egg production and egg quality, when compared to their parental breeds. Four genotypes AA, AM, MA and MM were compared and each consisted of 50 layer ducks. Each animal was kept in individual cage, and individual egg production and egg quality were recorded. They were given layer feed containing 20% crude protein and 3000 kcal/kg metabolisable energy. Results showed that the crossbred ducks (AM and MA laid eggs earlier than their parental breeds (AA and MM, and MA laid earlier than AM. The weight of first eggs of the crossbreeds did not differ significantly, AM was the same as MM and MA the same as AA. For the body weight at first lay, MA and AM weighed in between their parental breeds, AA was the heaviest and then followed by MA, AM and MM. For the 3-month egg production, MA ducks laid significantly more egg than the other 3 genotypes, and this is related to the age of first lay being the earliest. In terms of egg quality such as egg weight, yolk color, weight and thickness of shell, weight of the white and HU value, the crossbred ducks showed quality in between AA and MM. The AM ducks tended to be closer to MM and MA closer to AA, and this shows a strong influence of maternal effects. The average level of heterosis for weight of first egg is 2.41% and for 3-month egg production is 2.1%, but for MA cross the heterosis level is 11.69% for egg production. The overall results showed that crossbreeds between Alabio and Mojosari have the potential in improving production traits in the effort to increase productivity and production efficiency.

  10. Replacing soybean oil with increasing levels of soybean acid oil in diets of coturnix quails (Coturnix coturnix coturnix) and the effects on egg quality.

    Science.gov (United States)

    Roll, Aline A P; Forgiarini, Juliana; Xavier, Eduardo G; Lopes, Débora C N; Roll, Victor F B; Rutz, Fernando

    2018-01-01

    The effects of increasing levels of soybean acid oil in diets of quails on the quality of eggs were evaluated over a period of 56 days. A completely randomized design with five treatment levels of soybean acid oil (0, 2, 4, 6, and 8%) as a replacer for soybean oil and eight replicates of two quails each were used. A total of 240 fresh eggs were used for quality analysis, and 400 eggs were stored at 23ºC over a period of 0, 7, 14, 21, and 28 days for analysis of shelf-life. Polynomial regression and ANOVA with repeated measurements and Tukey´s tests were used. Soybean acid oil linearly increased the intensity of red (a* = -5.26 + 0.14 x, R2 = 0.89, P = 0.01) and yellow (b* = 42.32 + 0.44 x, R2 = 0.88 P = 0.01). All other variables of egg quality were not affected by the treatments, with the exception of sensory attributes, such as odor and aftertaste, which were more pronounced with soybean acid oil. In conclusion, soybean acid oil affects the intensity of yellow and red color of the yolk. Trained assessors detected differences in odor and aftertaste of eggs when soybean acid oil was included in the quail diet.

  11. Comparison of Aviary, Barn and Conventional Cage Raising of Chickens on Laying Performance and Egg Quality

    Directory of Open Access Journals (Sweden)

    M. Ahammed

    2014-08-01

    Full Text Available This study intended to compare the productive performance of three different layer raising systems; conventional cage (CC, barn (BR and aviary (AV. The AV is welfare bestowed housing that allows free locomotion for birds within the BR. The BR allows bird’s free locomotion inside BR but without multilevel structures. Both pullets and cockerels were housed together in both AV and BR, but only pullets in CC. Seventeen weeks old Lohmann Brown Lite (n = 800 pullets were housed in AV during this study. The same age layer pullets were simultaneously assigned to either at CC or BR to compare egg production performance with AV. The duration of experiment was 40 weeks (from 21st to 60th week. There were no remarkable differences in egg production, hen day egg production (HDEP and average egg weight among three rearing systems. First 20 weeks (phase-1 average HDEP (% of AV, CC, and BR were 85.9, 88.8, 87.1 and average egg weights (g were 57.5, 59.9, and 56.9 respectively. Those of the remaining 20 weeks (phase-2 were 87.1, 87.9, 85.5 and 64.2, 63.0 62.1, respectively. Daily feed intakes (122 g, 110 g, 125 g; feed conversion ratio (2.4, 2.1, 2.5 and daily egg mass (53.9 g, 54.4 g, 52.8 g data from AV, CC, and BR were not influenced significantly by the respective raising systems. Daily feed intake of layers in both AV (124 g and BR (127 g tended to be higher than that in CC (113 g during phase-2. Overall, exterior egg quality (dirty and cracked eggs in both phases was superior in BR compared with AV and CC, whereas CC generated intermediate results. This study indicated that the HDEP per se in AV and BR were not significantly different from that in CC. The study implied that the facility depreciation cost for AV and cost for increased feed intake in AV compared to CC are believed to be critical to evaluate the cost effectiveness of egg production in AV.

  12. [The external evaluation of study quality: the role in maintaining the reliability of laboratory information].

    Science.gov (United States)

    Men'shikov, V V

    2013-08-01

    The external evaluation of quality of clinical laboratory examinations was gradually introduced in USSR medical laboratories since 1970s. In Russia, in the middle of 1990 a unified all-national system of external evaluation quality was organized known as the Federal center of external evaluation of quality at the basis of laboratory of the state research center of preventive medicine. The main positions of policy in this area were neatly formulated in the guidance documents of ministry of Health. Nowadays, the center of external evaluation of quality proposes 100 and more types of control studies and permanently extends their specter starting from interests of different disciplines of clinical medicine. The consistent participation of laboratories in the cycles of external evaluation of quality intrinsically promotes improvement of indicators of properness and precision of analysis results and increases reliability of laboratory information. However, a significant percentage of laboratories does not participate at all in external evaluation of quality or takes part in control process irregularly and in limited number of tests. The managers of a number of medical organizations disregard the application of the proposed possibilities to increase reliability of laboratory information and limit financing of studies in the field of quality control. The article proposes to adopt the national standard on the basis of ISO 17043 "Evaluation of compliance. The common requirements of professional competence testing".

  13. The efficacy of using exogenous enzymes cocktail on production, egg quality, egg nutrients and blood metabolites of laying hens fed distiller's dried grains with solubles.

    Science.gov (United States)

    Abd El-Hack, M E; Chaudhry, M T; Mahrose, K M; Noreldin, A; Emam, M; Alagawany, M

    2018-04-01

    An experiment was performed using 120 Hisex Brown laying hens for evaluating the effects of different inclusion levels of corn distiller's dried grains with solubles (DDGS) as a replacement of soybean meal (SBM) with or without enzyme cocktail on performance, egg quality, egg nutrients and blood metabolites in laying hens through 22-42 weeks of age. A 4 × 2 factorial design experiment was performed including four substitution levels of DDGS (0, 250, 500 and 750 g/kg respectively) and two enzyme cocktail levels (0 and 250 mg/kg diet). The used enzyme in this study "Gallazyme" composed of xylanase, Trichoderma longibrachiatum (600 units/g), protease, Bacillus subtilis (8,000 units/g) and amylase and Bacillus amyloliquofaciens (800 units/g). The control diet showed the best feed efficiency followed by the intermediate levels of DDGS. The lowest value of feed efficiency was found in the group fed the highest level of DDGS. Enzyme addition improved feed efficiency and decreased laying rate. Increasing DDGS levels was associated with albumin and shell thickness increases. Dietary DDGS depressed all egg components except the organic matter which maximised in enzyme-treated groups. Increasing DDGS level was accompanied by increase in yolk cholesterol and total lipids. No significant impacts were detected with enzymes supplementation on yolk lipids profile. Excepting serum calcium and phosphorous, all serum constituents increased with increasing level of DDGS. Using enzyme markedly depressed serum ammonia by 15.02% and increased calcium by 6.44% compared with enzyme-free diets. Interaction between DDGS and enzyme was significant on most of studied parameters. It could be concluded that using enzyme cocktail in DDGS-based diets may improve feed efficiency and egg quality, in addition to lowering blood ammonia and increasing blood calcium. It is recommended to substitute SBM by DDGS up to 500 g/kg diet. © 2017 Blackwell Verlag GmbH.

  14. Trance, Transparency and Transformation: The Impact of External Quality Monitoring on Higher Education.

    Science.gov (United States)

    Stensaker, Bjorn

    2003-01-01

    Discusses the impact of external quality monitoring (EQM) on higher education and identifies areas in which changes have taken place as a result of such external initiatives. Suggests that the lack of effects related directly to quality improvement should not be conceived as an EQM design error, but rather as a misconception of how organizational…

  15. Quality control in the histopathology laboratory: An overview with stress on the need for a structured national external quality assessment scheme

    Directory of Open Access Journals (Sweden)

    Iyengar Jayaram

    2009-01-01

    Full Text Available The concept of quality control in histopathology is relatively young and less well understood. Like in other disciplines of laboratory medicine, the concept of quality and its control is applicable to pre analytical, analytical and post analytical activities. Assessment of both precision and accuracy performances is possible by appropriate internal and external quality control and assessment schemes. This article is a review of all processes that achieve quality reporting in histopathology. There is a special focus on external quality assessment - a scheme that lacks organization on a national level in our country. Statistical data derived from a small scale external quality assurance program is also analyzed along with recommendations to organize an effective national scheme with the participation of authorized zonal centers.

  16. Effects of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens

    Directory of Open Access Journals (Sweden)

    Xuemei Ding

    2017-06-01

    Full Text Available The study was conducted to investigate the effect of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens. A total of 960 Lohmann laying hens aged 53 weeks were enrolled, under 4 different treatment diets supplemented with 0, 50, 100 and 150 mg/kg essential oils (Enviva EO, Dupont Nutrition Biosciences ApS, Denmark, respectively. Each treatment was replicated 8 times with 30 birds each. Birds were fed dietary treatment diets for 12 weeks (54 to 65 weeks. For data recording and analysis, a 12-week period was divided into 3 periods of 4 weeks' duration each: period 1 (54 to 57 weeks, period 2 (58 to 61 weeks, and period 3 (62 to 65 weeks. For the diet supplemented with Enviva EO, hen-day egg production and the feed conversion ratio (FCR were significantly improved (P < 0.05 at weeks 58 to 61, and the eggshell thickness was significantly increased (P < 0.05 at week 65. However, egg production, egg weight, feed intake, FCR and other egg quality parameters (albumen height, Haugh unit, egg yolk color and eggshell strength were not affected by the dietary treatment. In addition, compared with the control diet, protein digestibility in the 100 mg/kg Enviva EO treatment group was significantly increased (P < 0.05, and fat digestibility in the 100 and 150 mg/kg Enviva EO treatment groups was significantly decreased (P < 0.05, but Enviva EO had no effect on energy apparent digestibility. Saturated fatty acid (SFA and monounsaturated fatty acid (MUFA gradually decreased and polyunsaturated fatty acid (PUFA increased with Enviva EO supplementation, but the difference was not significant. The data suggested that the supplementation of essential oils (Enviva EO in laying hen diet did not show a significant positive effect on performance and yolk fatty acid composition but it tended to increase eggshell thickness and protein digestibility, especially at the dose of 50 mg/kg.

  17. Egg shell and yolk quality characteristics of layers fed with sugarcane press residue in soya and fish based diets

    Directory of Open Access Journals (Sweden)

    N. Suma

    2015-02-01

    Full Text Available Aim: Sugarcane press residue (SPR, a by-product of sugarcane industry, which is rich in inorganic salts was assessed at different levels in both soya based and fish based diets of layers for egg shell and yolk quality characteristics. Materials and Methods: SPR was incorporated in 32-week-old white leghorn layer diets at 0%, 5%, 10% and 15% either in the soya based or fish based diets to form T1 to T8 diets, respectively. Each diet was offered to five replicates of four laying hens each constituting a total of one sixty birds kept for 84 days under colony cages. Results: Mean egg shell thickness obtained from eggs of experimental hens measured was 0.342, 0.329, 0.320, 0.322, 0.319, 0.332, 0.328 and 0.336 mm in T1 through T8 groups, respectively. About the main factor effects, both showed non-significant results. Similarly, influence of different treatment diets, in imparting colour to the yolks, was found to be non-significant (p>0.05 at different 28-day time intervals. Further, the average yolk index values ranged non-significantly from 0.360 (T6 to 0.383 (T4. Conclusion: The SPR can be incorporated into layer diet as a source of inorganic as well as organic nutrients without affecting its egg quality characteristics.

  18. Productive performance, egg quality, blood constituents, immune functions, and antioxidant parameters in laying hens fed diets with different levels of Yucca schidigera extract.

    Science.gov (United States)

    Alagawany, Mahmoud; Abd El-Hack, Mohamed E; El-Kholy, Mohamed S

    2016-04-01

    This study evaluated the effect of Yucca schidigera extract on productive performance, egg quality, blood metabolites, immune function, and antioxidant parameters in laying hens. A total of 96 36-week-old hens were allocated into four groups, the control diet or the diet supplemented with 50, 100, or 150 mg/kg of yucca extract, from 36 to 52 weeks of age. Hens were divided into four equal groups replicated six times with four hens per replicate. As a result of this study, there were no linearly or quadratically differences in body weight change (BWC), feed consumption (FC), feed conversion ratio (FCR), and egg weight (EW) due to yucca treatments at different ages, except FCR and EW that were improved with yucca supplementation during 36-40 weeks of age. Supplemental dietary yucca up to 100 mg/kg diet led to significant improvement in egg number (EN) and egg mass (EM). Egg qualities were not linearly or quadratically affected by yucca treatments except shell thickness was quadratically (P hen diets resulted in a significant linear (P feed additive to improve productive performance, blood profile, and antioxidant enzyme activities in laying hens.

  19. Effect of dietary γ-aminobutyric acid on laying performance, egg quality, immune activity and endocrine hormone in heat-stressed Roman hens.

    Science.gov (United States)

    Zhang, Min; Zou, Xiao-Ting; Li, Hui; Dong, Xin-Yang; Zhao, Wenjing

    2012-02-01

    This study was conducted to evaluate the effect of γ-aminobutyric acid (GABA) on laying performance, egg quality, digestive enzyme activity, hormone level and immune activities in Roman hens under heat stress. Roman hens (320 days old) were fed with 0, 25, 50, 75 and 100 mg/kg GABA, respectively during a 60-day experiment. Compared with control, supplementation of 50 mg/kg GABA improved the laying performance and egg quality by significantly increasing egg production, average egg weight and shell strength (P level. Anti-oxidation activity was improved by significantly increasing the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), but decreasing malondialdehyde level in serum (P level, follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E(2) ), insulin, triiodothyronine (T(3) ) and free triiodothyronine (FT(3) ) levels, and IgG, IgA and complement (C3)activity in serum (P GABA improved laying performance and physical condition mainly by modulating hormone secretion, enhancing anti-oxidation and immune activity, and maintaining electrolyte balance. Fifty mg/kg was the optimum level for laying hens under heat stress in the present study. © 2011 The Authors. Animal Science Journal © 2011 Japanese Society of Animal Science.

  20. Fermented feed for laying hens: effect on egg production, egg quality, plumage condition and composition and activity of the intestinal microflora

    DEFF Research Database (Denmark)

    Engberg, Ricarda M; Hammershøj, M; Johansen, N F

    2009-01-01

    1. An experiment with a total of 480 hens (Babcock) was carried out from 16 to 38 weeks of age to evaluate the suitability of wet fermented feed (feed water ratio, 1:1·2-1:1·4) for layers, taking aspects of nutrition and gastrointestinal health into consideration. The production performance, egg...... mash. Presumably because of an extended adaptation time to the feed, the onset of lay occurred later when hens were fed on fermented feed, resulting in non-significantly reduced total egg production (75 vs. 82%). 5. There was no significant difference between groups with respect to the total egg mass...... with the dry mash (g feed DM/g egg mass, 2·28 vs. 2·53). 6. The use of fermented feed increased egg weight in the period from 34 to 37 weeks (61·4 vs. 60·0) and increased shell weight (g/100 g egg weight, 10·2 vs. 9·9) and shell stiffness (N/mm, 161 vs. 150) of eggs collected at 37 weeks. 7. The feeding...

  1. Effect of dietary heated oil and garlic supplementation on the meat and egg quality of Japanese quail

    International Nuclear Information System (INIS)

    El-Faramawy, A.A.

    2006-01-01

    A total number of 360 Japanese quail (14 days old) were used to study the effect of using 2% garlic as a supplement in order to overcome the adverse effect of adding 4% heated oil on their meat and egg quality. The chicks were equally and randomly arranged into 4 groups, each of three replicates. The first and second groups were supplemented with 4% cotton seed oil and 4% cotton seed oil + 2% garlic, respectively, while in both third and forth groups, cotton seed oil was replaced by 4% heated oil. The body weight and mortality rate were examined weekly. At 42 day of age, 9 birds from each group were slaughtered. Serum cholesterol, triglycerides, T3 and T4 were determined by RIA besides to cholesterol and fatty acid in meat. The experiment was completed with the remaining chicks for another 6 weeks for analysis of egg cholesterol, triglyceride and fatty acids. The data revealed that addition of 2% garlic to both cotton seed and heated oil significantly decreased serum and egg cholesterol and triglycerides and meat cholesterol. In addition, total unsaturated fatty acid showed improvement in both meat and egg

  2. Replacing soybean oil with increasing levels of soybean acid oil in diets of coturnix quails (Coturnix coturnix coturnix and the effects on egg quality

    Directory of Open Access Journals (Sweden)

    ALINE A.P. ROLL

    2017-10-01

    Full Text Available ABSTRACT The effects of increasing levels of soybean acid oil in diets of quails on the quality of eggs were evaluated over a period of 56 days. A completely randomized design with five treatment levels of soybean acid oil (0, 2, 4, 6, and 8% as a replacer for soybean oil and eight replicates of two quails each were used. A total of 240 fresh eggs were used for quality analysis, and 400 eggs were stored at 23ºC over a period of 0, 7, 14, 21, and 28 days for analysis of shelf-life. Polynomial regression and ANOVA with repeated measurements and Tukey´s tests were used. Soybean acid oil linearly increased the intensity of red (a* = -5.26 + 0.14 x, R2 = 0.89, P = 0.01 and yellow (b* = 42.32 + 0.44 x, R2 = 0.88 P = 0.01. All other variables of egg quality were not affected by the treatments, with the exception of sensory attributes, such as odor and aftertaste, which were more pronounced with soybean acid oil. In conclusion, soybean acid oil affects the intensity of yellow and red color of the yolk. Trained assessors detected differences in odor and aftertaste of eggs when soybean acid oil was included in the quail diet.

  3. Enhancement of crystal homogeneity of protein crystals under application of an external alternating current electric field

    Energy Technology Data Exchange (ETDEWEB)

    Koizumi, H.; Uda, S.; Fujiwara, K.; Nozawa, J. [Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577 (Japan); Tachibana, M. [Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027 (Japan); Kojima, K. [Department of Education, Yokohama Soei University, 1 Miho-tyou, Midori-ku, Yokohama, 226-0015 (Japan)

    2014-10-06

    X-ray diffraction rocking-curve measurements were performed on tetragonal hen egg white (HEW) lysozyme crystals grown with and without the application of an external alternating current (AC) electric field. The crystal quality was assessed by the full width at half maximum (FWHM) value for each rocking curve. For two-dimensional maps of the FWHMs measured on the 440 and the 12 12 0 reflection, the crystal homogeneity was improved under application of an external electric field at 1 MHz, compared with that without. In particular, the significant improvement of the crystal homogeneity was observed for the 12 12 0 reflection.

  4. Avian Egg and Egg Coat.

    Science.gov (United States)

    Okumura, Hiroki

    2017-01-01

    An ovulated egg of vertebrates is surrounded by unique extracellular matrix, the egg coat or zona pellucida, playing important roles in fertilization and early development. The vertebrate egg coat is composed of two to six zona pellucida (ZP) glycoproteins that are characterized by the evolutionarily conserved ZP-domain module and classified into six subfamilies based on phylogenetic analyses. Interestingly, investigations of biochemical and functional features of the ZP glycoproteins show that the roles of each ZP-glycoprotein family member in the egg-coat formation and the egg-sperm interactions seemingly vary across vertebrates. This might be one reason why comprehensive understandings of the molecular basis of either architecture or physiological functions of egg coat still remain elusive despite more than 3 decades of intensive investigations. In this chapter, an overview of avian egg focusing on the oogenesis are provided in the first section, and unique features of avian egg coat, i.e., perivitelline layer, including the morphology, biogenesis pathway, and physiological functions are discussed mainly on chicken and quail in terms of the characteristics of ZP glycoproteins in the following sections. In addition, these features of avian egg coat are compared to mammalian zona pellucida, from the viewpoint that the structural and functional varieties of ZP glycoproteins might be associated with the evolutionary adaptation to their reproductive strategies. By comparing the egg coat of birds and mammals whose reproductive strategies are largely different, new insights into the molecular mechanisms of vertebrate egg-sperm interactions might be provided.

  5. eggNOG v4.0

    DEFF Research Database (Denmark)

    Powell, Sean; Forslund, Kristoffer; Szklarczyk, Damian Milosz

    2013-01-01

    levels compared with 41 in eggNOGv3, (iii) identification and annotation of particularly closely related orthologous groups, facilitating analysis of related gene families, (iv) improvements of the clustering and functional annotation approach, (v) adoption of a revised tree building procedure based...... prioritizing the inclusion of high-quality genomes to minimize error propagation from incomplete proteome sets. Major technological advances include (i) a robust and scalable procedure for the identification and inclusion of high-quality genomes, (ii) provision of orthologous groups for 107 different taxonomic...... on the multiple alignments generated during the process and (vi) implementation of quality control procedures throughout the entire pipeline. As in previous versions, eggNOGv4 provides multiple sequence alignments and maximum-likelihood trees, as well as broad functional annotation. Users can access the complete...

  6. Effects of Divergent Selection Body Weight and the Quail Laying Eggs on some Physical and Mechanical Properties of Japanese Quail Eggs

    Directory of Open Access Journals (Sweden)

    mousareza baghani

    2016-08-01

    Full Text Available Introduction Breeding quail and quail egg industrial production because of the high nutritional value of these products has been developing these years. Considering the high demand and economical production, further development in the future is predicted. In order to design and effective utilization of the equipment for transportation, separation, packaging and storage, the physical and mechanical properties of quail eggs are needed. It is of great importance to study the factors which are influencing these properties. Strength of the shell is an important factor in determination of quail egg quality. Quail egg shell strength depends on several variables including specific gravity, egg weight and volume, shell thickness, weight and percentage of shell, hardness, breaking force, breaking energy, egg surface, farming conditions, type and species of birds, nutrition and geometric characteristics of eggs. Materials and Methods In this paper, initially in three phases, at 15 weeks, 19 weeks and 23 weeks, shell strength changes and physical properties of quail eggs on 90 quails during their first period of laying eggs were studied. Measured properties were included dimensions, weight, volume, specific gravity, shell thickness, weight and percentage of shell, breaking force, breaking energy, egg surface and some other properties. In the second part the effects of divergent selection for the bird’s body weight at four weeks of age on the quail eggs of fifth generation were studied. Measured properties were included dimensions, weight, volume, breaking force, breaking energy and some factors for direct and indirect measurement of strength of quail eggs. For direct measurement of the strength of eggshell of quail, two methods were employed: The first method was quasi-static compression test between two parallel plates of the universal testing machine and the second method was measuring specific gravity. In the first method, eggs were compressed between

  7. Egg Shell and Oyster Shell Powder as Alternatives for Synthetic Phosphate: Effects on the Quality of Cooked Ground Pork Products.

    Science.gov (United States)

    Cho, Min Guk; Bae, Su Min; Jeong, Jong Youn

    2017-01-01

    This study aimed to determine the optimal ratio of natural calcium powders (oyster shell and egg shell calcium) as synthetic phosphate replacers in pork products. Ground pork samples were subjected to six treatments, as follows: control (-) (no phosphate added), control (+) (0.3% phosphate blend added), treatment 1 (0.5% oyster shell calcium powder added), treatment 2 (0.3% oyster shell calcium powder and 0.2% egg shell calcium powder added), treatment 3 (0.2% oyster shell calcium powder and 0.3% egg shell calcium powder added), and treatment 4 (0.5% egg shell calcium powder added). The addition of natural calcium powders resulted in an increase in the pH values of meat products, regardless of whether they were used individually or mixed. The highest cooking loss was observed ( p cooking loss in samples with natural calcium powder added was similar ( p >0.05) to that in the positive control samples. CIE L* values decreased as the amount of added egg shell calcium powder increased. CIE a* values were higher ( p egg shell powder (treatment 2 or 3) was effective for the improvement of textural properties of the pork products. The findings show that the combined use of 0.2% oyster shell calcium and 0.3% egg shell calcium should enable the replacement of synthetic phosphate in the production of cooked pork products with desirable qualities.

  8. Effect of dietary supplementation of organic zinc on laying performance, egg quality and some biochemical parameters of laying hens.

    Science.gov (United States)

    Abd El-Hack, M E; Alagawany, M; Amer, S A; Arif, M; Wahdan, K M M; El-Kholy, M S

    2018-04-01

    This study was conducted to evaluate the effects of zinc methionine (Zn-Met) supplementation on the performance, egg quality, antioxidant status and some biochemical parameters of blood serum in laying hens from 22 to 34 weeks of age. A total of 120 Hisex Brown laying hens of 22-week-old were randomly allocated into five treatments with six cage replicates for each (four hens/replicate). Dietary treatments consisted of the basal diet with no Zn-Met supplementation (control group) and basal diet supplemented with 25, 50, 75 or 100 mg Zn-Met/kg diet. No significant differences were observed on body weight, body weight gain or feed conversion ratio due to dietary Zn-Met supplementation. However, highly significant impact was observed on daily feed intake. Egg number, egg weight and egg mass were increased in the group fed diet supplemented with the highest level of Zn-Met (100 mg/kg of diet) as compared to other groups. All egg quality traits were statistically (p > .05 or .01) affected as a response to dietary Zn-Met supplementation except egg shape index, shell percentage and yolk index. In comparison with the control group, dietary supplementation of 25, 50, 75 or 100 mg Zn-Met/kg decreased serum triglyceride and LDL-cholesterol levels. Serum cholesterol level was increased with all dietary levels of Zn-Met in comparison with the control group. Dietary Zn-Met supplementation increased the serum content of zinc, where the highest values were recorded with 50 and 100 mg Zn-Met/kg diet. Dietary Zn-Met levels did not affect the antioxidant indices in blood serum except for the activity of copper-zinc superoxide dismutase (Cu-Zn-SOD). The activity of Cu-Zn-SOD was increased with Zn-Met supplementations with no differences among supplemental zinc levels. It is concluded that dietary Zn-Met supplementation reduced serum triglyceride, LDL-cholesterol and increased Zn status and resulted in promoting antioxidant ability of laying hens, and the addition of 100 mg Zn

  9. Intraspecific variation in egg size and egg composition in birds: effects on offspring fitness.

    Science.gov (United States)

    Williams, T D

    1994-02-01

    involve variation in lipid content, and therefore in egg 'quality.' The adaptive significance of variation in egg composition is considered; females may adjust egg composition facultatively to maximise the benefits to their offspring of increased reproductive investment. 5. Considerations for future research are discussed with particular emphasis on experimental studies and the application of new techniques.

  10. Competitiveness of the EU egg sector, base year 2015

    NARCIS (Netherlands)

    Horne, van P.L.M.; Bondt, N.

    2017-01-01

    In this report the impact of reducing or removing import levies on the competitiveness of the EU egg sector is studied, for both shell eggs and whole egg powder. As a result of the costs of transportation, import levies and the effects on product quality and safety, there will barely be imports of

  11. Migration and income transfers in the presence of labor quality externalities

    OpenAIRE

    Huizinga, H.P.

    1994-01-01

    In this paper, a worker's productivity is assumed to depend on his own quality and on the average quality of other workers in the same country. The external effects associated with worker quality give rise to increasing returns to average worker quality. As a result, free migration generally reduces world output. Within each country, social benefits that induce low quality workers to leave the labor force can increase national income. Moreover, the operation of such a benefit scheme financed ...

  12. The Fifty Year Rehabilitation of the Egg

    Directory of Open Access Journals (Sweden)

    Donald J. McNamara

    2015-10-01

    Full Text Available The 1968 American Heart Association announced a dietary recommendation that all individuals consume less than 300 mg of dietary cholesterol per day and no more than three whole eggs per week. This recommendation has not only significantly impacted the dietary patterns of the population, but also resulted in the public limiting a highly nutritious and affordable source of high quality nutrients, including choline which was limited in the diets of most individuals. The egg industry addressed the egg issue with research documenting the minimal effect of egg intake on plasma lipoprotein levels, as well as research verifying the importance of egg nutrients in a variety of issues related to health promotion. In 2015 dietary cholesterol and egg restrictions have been dropped by most health promotion agencies worldwide and recommended to be dropped from the 2015 Dietary Guidelines for Americans.

  13. 7 CFR 1250.306 - Commercial eggs or eggs.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Commercial eggs or eggs. 1250.306 Section 1250.306... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE EGG RESEARCH AND PROMOTION Egg Research and Promotion Order Definitions § 1250.306 Commercial eggs or eggs. Commercial eggs or eggs means...

  14. Effects of Xylem-Sap Composition on Glassy-Winged Sharpshooter (Hemiptera: Cicadellidae) Egg Maturation on High- and Low-Quality Host Plants.

    Science.gov (United States)

    Sisterson, Mark S; Wallis, Christopher M; Stenger, Drake C

    2017-04-01

    Glassy-winged sharpshooters must feed as adults to produce mature eggs. Cowpea and sunflower are both readily accepted by the glassy-winged sharpshooter for feeding, but egg production on sunflower was reported to be lower than egg production on cowpea. To better understand the role of adult diet in egg production, effects of xylem-sap chemistry on glassy-winged sharpshooter egg maturation was compared for females confined to cowpea and sunflower. Females confined to cowpea consumed more xylem-sap than females held on sunflower. In response, females held on cowpea produced more eggs, had heavier bodies, and greater lipid content than females held on sunflower. Analysis of cowpea and sunflower xylem-sap found that 17 of 19 amino acids were more concentrated in cowpea xylem-sap than in sunflower xylem-sap. Thus, decreased consumption of sunflower xylem-sap was likely owing to perceived lower quality, with decreased egg production owing to a combination of decreased feeding and lower return per unit volume of xylem-sap consumed. Examination of pairwise correlation coefficients among amino acids indicated that concentrations of several amino acids within a plant species were correlated. Principal component analyses identified latent variables describing amino acid composition of xylem-sap. For females held on cowpea, egg maturation was affected by test date, volume of excreta produced, and principal components describing amino acid composition of xylem-sap. Principal component analyses aided in identifying amino acids that were positively or negatively associated with egg production, although determining causality with respect to key nutritional requirements for glassy-winged sharpshooter egg production will require additional testing. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.

  15. Diseases of amphibian eggs and embryos

    Science.gov (United States)

    Green, D.E.; Converse, K.A.; Majumdar, S.K.; Huffman, J.E.; Brenner, F.J.; Panah, A.I.

    2005-01-01

    female oviduct, either due to infectious disease, nutritional status, hormonal imbalances, or sublethal intoxications, could affect the quality of secreted gelatinous capsules on eggs, thus rendering an egg mass susceptible to other stressors. Diseases of amphibian eggs and embryos presented in this chapter are Lucke frog herpesvirus Ranavirus (iridovirus) infection Bacteria Watermold infection (saprolegniasis) Algae Microsporidia

  16. Influence of greenhouse climate and plant density on external quality of chrysanthemum (Dendranthema grandiflorum (Ramat.)Kitamura) : First steps towards a quality model

    NARCIS (Netherlands)

    Carvalho, S.M.P.; Heuvelink, E.

    2001-01-01

    The effects of greenhouse climate and plant density on external quality of chrysanthemum (Dendranthema grandiflorum syn. Chrysanthemum morifolium) are reviewed. The external quality aspects analysed in this paper are stem morphology (length, diameter and "strength"), leaf morphology (number and

  17. Short- and long-term effects of egg size and feeding frequency on offspring quality in the collared flycatcher (Ficedula albicollis).

    Science.gov (United States)

    Krist, Milos

    2009-09-01

    1. Despite the central importance for life-history theory, egg-size effects on offspring fitness are still considered ambiguous. Most previous studies were only observational and consequently might suffer from uncontrolled correlations between egg size and parental/territory quality. Even after cross-fostering is performed, direct genetic effects and parental adjustment of post-natal care might confound our estimates of egg-size effects per se. 2. I performed a full cross-fostering experiment in the collared flycatcher (Ficedula albicollis) exchanging the whole clutches between pairs of nests. I statistically controlled for direct genetic effects and parental feeding frequencies. I followed young until recruitment to estimate the long-term effects of egg size and parental provisioning. In addition, I compared the effects obtained in the cross-fostering experiment with those obtained from a set of unmanipulated nests. 3. Egg size per se affected offspring morphology in both the short and long term, while having no effect on offspring survival and immunity. Egg-size effects were not confounded by parental post-natal care and direct genetic effects. 4. The number of care-givers was an influential predictor of nestling performance. Apart from the variation caused by this factor, feeding frequencies had no consistent effect on offspring performance. 5. Fitness benefits of large eggs may be difficult to establish due to variation of egg-size effects between years and habitats. Feeding frequency may affect offspring state but offspring state may also affect feeding frequency. Varying causality between feeding rate and offspring state may preclude the detection of a positive effect of the former on the latter.

  18. [Analysis of the results of the SEIMC External Quality Control Program. Year 2013].

    Science.gov (United States)

    de Gopegui Bordes, Enrique Ruiz; Orta Mira, Nieves; Del Remedio Guna Serrano, M; Medina González, Rafael; Rosario Ovies, María; Poveda, Marta; Gimeno Cardona, Concepción

    2015-07-01

    The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) include controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology, molecular microbiology and HIV-1, HCV and HBV viral loads. This manuscript presents the analysis of results obtained of the participants from the 2013 SEIMC External Quality Control Programme, except viral loads controls, that they are summarized in a manuscript abroad. As a whole, the results obtained in 2013 confirm the excellent skill and good technical standards found in previous editions. However, erroneous results can be obtained in any laboratory and in clinically relevant determinations. Once again, the results of this program highlighted the need to implement both internal and external controls in order to assure the maximal quality of the microbiological tests. Copyright © 2015 Elsevier España, S.L.U. All rights reserved.

  19. Effect of limestone particle size on egg production and eggshell ...

    African Journals Online (AJOL)

    Different limestone particle sizes had no effect on any of the tested egg production and eggshell quality parameters. These results suggested that larger particles limestone are not necessarily essential to provide sufficient Ca2+ to laying hens for egg production and eggshell quality at end-of-lay, provided that the dietary Ca ...

  20. Effects of rice bran on performance, egg quality, oxidative status, yolk fatty acid composition, and fatty acid metabolism-related gene expression in laying ducks.

    Science.gov (United States)

    Ruan, D; Lin, Y C; Chen, W; Wang, S; Xia, W G; Fouad, A M; Zheng, C T

    2015-12-01

    The study was designed to evaluate the effects of different dietary levels of rice bran (RB) in laying duck diets on performance, egg quality, oxidation status, egg yolk fatty acid composition, and hepatic expression of fatty acid metabolism-related genes. Longyan females (1080) with similar BW at 19 wk of age were randomly assigned to 6 dietary treatments, each consisting of 6 replicates of 30 birds. The basal diet (I) was a typical corn-soybean ration while the experimental diets (II to VI) substituted RB for corn and wheat bran and a small reduction of soybean meal. The level of substitution in diets (II to VI) was 6%, 12%, 18%, 24%, and 30%, respectively. The experiment lasted for 12 wks. Average egg weight and daily egg mass decreased linearly as the level of RB inclusion increased (Pegg yolk linearly decreased with increasing RB, and many of the key polyunsaturated fatty acids (PUFA), like C18:2 n-6 and C18:3 n-3, linearly increased (Pegg yolk cholesterol or triglyceride content (P>0.05). In conclusion, the current study suggests that ducks from 19 to 31 wk could be fed diets with up to about 18% RB without effect on the number of eggs produced, egg quality, and oxidative status. Increasing amounts of RB linearly increased egg yolk concentrations of key fatty acids like C18:2 n-6 and C18:3 n-3 and decreased the hepatic abundance of FAS and SREBP-1 transcripts. © 2015 Poultry Science Association Inc.

  1. Study on the sterilization of egg white powder by irradiation

    International Nuclear Information System (INIS)

    Fu Lixin; Meng Lifen; Zhao Xiaonan; Xu Dechun; Hu Shaoxin; Chen Changdong; Wang Zhidong

    2013-01-01

    The sterilizing effect, influence on main essential nutrient and sensory qualities of egg white powder treated with 60 Co γ-ray irradiation were studied. The microorganism index of egg white powder samples treated with 4 kGy irradiation could meet the government food sanitation standard, and the sterilization rate could reach 100%. The irradiation doses from 4 to 10 kGy had little influence on egg white powder protein, crude fiber and amino acidscontent, and the sensory quality such as color, luster, and scent changed little below 6 kGy irradiation. Considering the result of this study, the optimal irradiation dose for egg white powder sterilization was 4 to 6 kGy. (authors)

  2. Effect of fluorescent vs. poultry-specific light-emitting diode lights on production performance and egg quality of W-36 laying hens.

    Science.gov (United States)

    Liu, Kai; Xin, Hongwei; Sekhon, Jasreen; Wang, Tong

    2018-03-01

    More energy-efficient, durable, affordable, and dimmable light-emitting diode (LED) lights are finding applications in poultry production. However, data are lacking on controlled comparative studies concerning the impact of such lights during the pullet rearing and subsequent laying phase. This study evaluated two types of poultry-specific LED light (PS-LED) vs. fluorescent light (FL) with regards to their effects on hen laying performance. A total of 432 Hy-Line W-36 laying hens were tested in two batches using four environmental chambers (nine cages per chamber and 6 birds per cage) from 17 to 41 weeks of age (WOA). Dim-to-red PS-LED and warm-white FL were used in the laying phase. The hens had been reared under a dim-to-blue PS-LED or a warm-white FL from 1 to 16 WOA. The measured performance variables included 1) timing of sexual maturity, 2) egg production performance, 3) egg quality, and 4) egg yolk cholesterol. Results showed that the two types of light used during the laying phase had comparable performance responses for all response parameters (P > 0.05) with a few exceptions. Specifically, eggs laid from hens in the PS-LED treatment had lower shell thickness (P = 0.01) and strength (P = 0.03) than those in the FL treatment at 41 WOA. The two types of light used during the rearing phase did not influence the 17 to 41 WOA laying performance, except that hens reared under the PS-LED laid eggs with lower shell thickness (P = 0.02) at 32 WOA as compared to hens reared under the FL. This study demonstrates that the emerging poultry-specific LED lights yield comparable production performance and egg quality of W-36 laying hens to the traditional fluorescent lights.

  3. Effects of growth conditions on external quality of cut chrysanthemum; analysis and simulation

    NARCIS (Netherlands)

    Carvalho, S.M.P.

    2003-01-01

    For many years the emphasis in floricultural research laid with quantity rather than quality. Nowadays, since the prices are often determined on the basis of visual quality aspects, the so-called external quality, chrysanthemum growers aim to provide a high and constant product quality throughout

  4. Child diurnal cortisol rhythms, parenting quality, and externalizing behaviors in preadolescence.

    Science.gov (United States)

    Martin, Christina Gamache; Kim, Hyoun K; Bruce, Jacqueline; Fisher, Philip A

    2014-02-01

    This study examined a neurobiologically informed model of the emergence of child externalizing behaviors in an ethnically diverse community sample of 232 9-12 year old children. Replicating extensive prior research, our analyses revealed that parents' inconsistent discipline and poor quality monitoring were predictive of child externalizing behavior. In addition, poor parental monitoring, but not inconsistent discipline, was associated with children having a significantly flatter morning-to-evening cortisol slope, which was in turn, related to higher levels of externalizing behaviors. An indirect effect of parental monitoring on externalizing behaviors, through child diurnal cortisol rhythms, was also supported. These findings highlight the role of the hypothalamic-pituitary-adrenal (HPA) axis and its hormonal end product, cortisol, in the relationship between the caregiving environment and the development of externalizing behaviors. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. It's what's inside that counts: egg contaminant concentrations are influenced by estimates of egg density, egg volume, and fresh egg mass.

    Science.gov (United States)

    Herzog, Mark P; Ackerman, Joshua T; Eagles-Smith, Collin A; Hartman, C Alex

    2016-05-01

    In egg contaminant studies, it is necessary to calculate egg contaminant concentrations on a fresh wet weight basis and this requires accurate estimates of egg density and egg volume. We show that the inclusion or exclusion of the eggshell can influence egg contaminant concentrations, and we provide estimates of egg density (both with and without the eggshell) and egg-shape coefficients (used to estimate egg volume from egg morphometrics) for American avocet (Recurvirostra americana), black-necked stilt (Himantopus mexicanus), and Forster's tern (Sterna forsteri). Egg densities (g/cm(3)) estimated for whole eggs (1.056 ± 0.003) were higher than egg densities estimated for egg contents (1.024 ± 0.001), and were 1.059 ± 0.001 and 1.025 ± 0.001 for avocets, 1.056 ± 0.001 and 1.023 ± 0.001 for stilts, and 1.053 ± 0.002 and 1.025 ± 0.002 for terns. The egg-shape coefficients for egg volume (K v ) and egg mass (K w ) also differed depending on whether the eggshell was included (K v  = 0.491 ± 0.001; K w  = 0.518 ± 0.001) or excluded (K v  = 0.493 ± 0.001; K w  = 0.505 ± 0.001), and varied among species. Although egg contaminant concentrations are rarely meant to include the eggshell, we show that the typical inclusion of the eggshell in egg density and egg volume estimates results in egg contaminant concentrations being underestimated by 6-13 %. Our results demonstrate that the inclusion of the eggshell significantly influences estimates of egg density, egg volume, and fresh egg mass, which leads to egg contaminant concentrations that are biased low. We suggest that egg contaminant concentrations be calculated on a fresh wet weight basis using only internal egg-content densities, volumes, and masses appropriate for the species. For the three waterbirds in our study, these corrected coefficients are 1.024 ± 0.001 for egg density, 0.493 ± 0.001 for K v , and 0.505 ± 0.001 for K w .

  6. Irradiation of liquid egg, frozen egg, powdered egg, egg yolk and white of egg: reducing the population of Salmonella enteritidis and sensory aspects and physico-chemical

    International Nuclear Information System (INIS)

    Froehlich, Angela

    2004-01-01

    Eggs and their products have been incriminated in foodborne disease outbreaks due to Salmonella enteritidis contamination. Irradiation is a food preservation technology that could be applied to minimize the problem. The aim of this study was to determine the effect of irradiation in liquid and frozen egg as well as in powdered egg, egg yolk and egg white spiked with Salmonella enteritidis. Spiked samples of liquid egg, egg white and egg yolk were exposed to 0,5; 1,0; 1,5; 2,0; 2,5; 3,0 kGy and spiked samples of frozen and powdered egg were exposed to 0,5; 1,0; 1,5; 2,0; 2,5; 3,0; 3,5 e 4,0 kGy. Raw odour, cooked odour and taste of non inoculated and irradiated samples and non irradiated samples of egg and egg products were analysed by a trained penal. Viscosity and lipid oxidation (malonaldehyde concentration) were also determined. Doses of 2,0; 3,0; 3,5; 3,0 e 3,5 kGy reduced in 5 log the population of S. Enteritidis in liquid and frozen egg, powdered egg yolk, egg white and egg, respectively, with moderate alterations in relation to non irradiated samples detected by the trained penal. Viscosity and lipid oxidation in the powdered products, however, showed more intense alterations. Therefore, irradiation can be considered a feasible process for liquid and frozen egg while when applied to powdered products it should be considered the type of food product to which they will be added due to alterations in viscosity. (author)

  7. Effect of diatomaceous earth on parasite load, egg production, and egg quality of free-range organic laying hens.

    Science.gov (United States)

    Bennett, D C; Yee, A; Rhee, Y-J; Cheng, K M

    2011-07-01

    The effectiveness of diatomaceous earth (DE) as a treatment against parasites and to increase feed efficiency and egg production of organically raised free-range layer hens was evaluated in 2 breeds of commercial egg layers [Bovan Brown (BB) and Lowmann Brown (LB)] that differ in their resistance to internal parasitic infections. Half the hens of each breed were fed diets supplemented with DE (2%). Their internal parasite loads were assessed by biweekly fecal egg counts (FEC) and by postmortem examination of the gastrointestinal tract. Supplementing DE in diets of LB hens, the more parasite-resistant breed, did not significantly affect their FEC and adult parasite load. However, BB hens treated with dietary DE had significantly lower Capillaria FEC, slightly lower Eimeria FEC, fewer birds infected with Heterakis, and significantly lower Heterakis worm burden than control BB hens. Both BB and LB hens fed the diet containing DE were significantly heavier, laid more eggs, and consumed more feed than hens fed the control diet, but feed efficiency did not differ between the 2 dietary treatments. Additionally, BB hens consuming the DE diet laid larger eggs containing more albumen and yolk than hens consuming the control diet. In a subsequent experiment, the effectiveness of DE to treat a Northern fowl mite (Ornithonyssus sylviarum) infestation was tested. Relative to controls, both breeds of hens that were dusted with DE had reduced number of mites. The results of this study indicate the DE has the potential to be an effective treatment to help control parasites and improve production of organically raised, free-range layer hens.

  8. Using a feed-grade zinc propionate to achieve molt induction in laying hens and retain postmolt egg production and quality.

    Science.gov (United States)

    Park, S Y; Kim, W K; Birkhold, S G; Kubena, L F; Nisbet, D J; Ricke, S C

    2004-11-01

    A commercial-feed-grade form of zinc propionate was examined as a potential feed amendment at a concentration of 1% zinc to induce molt in 90-wk-old hens. Dietary treatments consisted of 4 treatment groups of 28 birds each randomly assigned to either (1) molted conventionally by feed withdrawal, (2) 1% zinc as Zn acetate, (3) 1% zinc as Zn propionate, or (4) nonmolted control for 9 d. Ovary weights of hens fed Zn acetate or Zn propionate were not significantly different from each other, but hens fed Zn acetate or Zn propionate were significantly (phens. Zinc concentrations in the kidney and liver were significantly (phens when compared to either nonmolted control-fed hens or feed-withdrawal molted hens. Over the entire 3-mo postmolt period, there were no significant differences in interior or exterior egg qualities among the four treatments. Egg production of hens fed Zn acetate was significantly lower than feed-withdrawal hens, Zn propionate-fed hens, or nonmolted control hens (pfeeding a feed grade of Zn propionate (1% Zn)-supplemented diet can induce molt and retain postmolt egg quality and production comparable to hens molted by feed withdrawal.

  9. Impacts of radiation processing on physicochemical properties of Table Eggs

    International Nuclear Information System (INIS)

    Farag, M. D. H.; Abdul Azeem, A. M.

    2012-12-01

    This study investigated the effect of gamma irradiation on pathogens, quality, and functional properties of shell eggs. Using intact, fresh while and brown shell eggs, inoculated with 10 9 colony-forming units (cfu) of S enteritidis and S, typhimurium. The effect of gamma-irradiation at doses 2,4 and 6 kGy of gamma irradiation on bacteriologic and population and physical characteristics (Haugh units and yolk color), chemical composition (moisture, crude protein, crude fat and ash), the rheological changes (viscosity), pH and protean solubility of the eggs were determinate d. Results showed that 2 kGy, the number of S, enteritidis and eggs internal quality as freshness as measured by albumen height and the number of hugh units, and yolk color index were all significantly reduced with increasing irradiation doses. On significant change was found after irradiation in egg white or yolk in the chemical composition (moisture, crude protein, crude fat and ash). After irradiation, the foaming ability and foam capacity of egg white were increasing radiation doses> The viscosity of egg white and yolk decreased with increasing doses of irradiation. The pH of the egg white and yolk increasing doses of radiation dose. The protein salability decreased significantly in egg white and yolk with increasing radiation dose. These Results Suggest that gamma irradiation reduce the freshness of shell eggs and improving important functional properties such as to foaming ability and foaming capacity. Gamma irradiation can also be applied to the egg breaking process sine irradiation reduces the viscosity of egg white and yolk, which can allow egg whites and yolks to be separated with greater efficiency. (Author)

  10. Fontes de lipídios na dieta de poedeiras: desempenho produtivo e qualidade dos ovos Lipids sources on layer hen diet: performance and egg quality

    Directory of Open Access Journals (Sweden)

    D.D. Oliveira

    2010-06-01

    Full Text Available Os efeitos de fontes de lipídios na dieta de poedeiras sobre o desempenho produtivo e a qualidade do ovo foram verificados em dois experimentos. Foram utilizados quatro tratamentos, definidos de acordo com a fonte lipídica adicionada nas rações: óleo de soja, óleo de girassol, óleo de linhaça e ração controle (sem adição de óleo. Os experimentos foram realizados com poedeiras de 20 e 54 semanas de idade, respectivamente, com duração de oito semanas. Avaliaram-se os parâmetros produtivos - consumo de ração, conversão alimentar, produção e peso dos ovos - e de qualidade do ovo - porcentagens de gema, albúmen e casca, sólidos da gema, unidades Haugh, pH do albúmen, e cor da gema. Somente o peso do ovo de poedeiras jovens foi afetado pela inclusão de óleos vegetais na ração. Esses ovos apresentaram maior peso e maior porcentagem de gema que os produzidos pelas poedeiras jovens alimentadas com ração-controle. Concluiu-se que a utilização de diferentes fontes de lipídios na ração de poedeiras não altera o desempenho produtivo e a qualidade interna dos ovos, com exceção do peso do ovo de poedeiras novas, que aumenta com a utilização de óleos vegetais.The effects of different sources of fat in the diet of layer hens on performance and egg quality were recorded in two experiments. Four treatments were defined by the lipid source added to the diets: soybean oil, sunflower oil, linseed oil and control diet (without oil added. The experiments were carried out with layers from 20 to 54 weeks of age, respectively, for eight weeks. Production parameters (feed intake, feed conversion, egg production, and egg weight and quality of eggs (percentages of yolk, albumen, and shell; total solids of yolk; Haugh units; pH of albumen; and yolk color were evaluated. Among the parameters of production and quality of the eggs, only the weight of eggs and percentage of yolk of young hens were affected by the inclusion of vegetable

  11. Cracking the egg: An insight into egg hypersensitivity.

    Science.gov (United States)

    Dhanapala, Pathum; De Silva, Chamika; Doran, Tim; Suphioglu, Cenk

    2015-08-01

    Hypersensitivity to the chicken egg is a widespread disorder mainly affecting 1-2% of children worldwide. It is the second most common food allergy in children, next to cow's milk allergy. Egg allergy is mainly caused by hypersensitivity to four allergens found in the egg white; ovomucoid, ovalbumin, ovotransferrin and lysozyme. However, some research suggests the involvement of allergens exclusively found in the egg yolk such as chicken serum albumin and YGP42, which may play a crucial role in the overall reaction. In egg allergic individuals, these allergens cause conditions such as itching, atopic dermatitis, bronchial asthma, vomiting, rhinitis, conjunctivitis, laryngeal oedema and chronic urticaria, and anaphylaxis. Currently there is no permanent cure for egg allergy. Upon positive diagnosis for egg allergy, strict dietary avoidance of eggs and products containing traces of eggs is the most effective way of avoiding future hypersensitivity reactions. However, it is difficult to fully avoid eggs since they are found in a range of processed food products. An understanding of the mechanisms of allergic reactions, egg allergens and their prevalence, egg allergy diagnosis and current treatment strategies are important for future studies. This review addresses these topics and discusses both egg white and egg yolk allergy as a whole. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. The Effect Used at Egg Preservation Using Paraffin and the Use of Sodium Bicarbonate on Physical, Chemical and Organoleptical Characteristcs of Egg Crackers

    Directory of Open Access Journals (Sweden)

    Zulfatunnisa Zulfatunnisa

    2017-03-01

    Full Text Available The materials used in this research were egg crackers made from albumen, tapioca, wheat flour, garlic, salt, water and sodium bicarbonate. The variables measured were expansion power, breaking force, water content, protein content, mineral content and organoleptic quality (colour, flavour, crispiness. Data were analized with analysis of variance and followed by Duncan's Multiple Range Test.The result showed that the preservation with paraffin and the use of sodium bicarbonate had a highly significant effect (P<0.01 on expansion power, breaking force, water content, protein content, minerals content and organoleptic quality (colour, flavour and crispiness. It's interaction had a highly significant effect (P<0.01 on protein content and organoleptic quality (flavour and crispiness of eggcrackers. It is found that egg coated with paraffin can be kept for 60 days had highfoaming foroe similar value with egg without preservation however, it haddisadvantages on expansion poweq breaking force and colour than fresh egg, so suggested to improve by use increase quantity albumen and decrease the Use of Sodium bicarbonate.

  13. First chelonian eggs and carapace fragments from the Pliocene of Rhodes, Greece

    DEFF Research Database (Denmark)

    Mueller-Töwe, Inken J.; Kjeldahl-Vallon, Tina A.; Milàn, Jesper

    2011-01-01

    Well-preserved fossil eggs and eggshell fragments from the Pliocene Apolakkia Formation of Rhodes (Greece) are described. The eggs were found in-situ in a clutch. They are sub-spherical with lengths of 53-60 mm and widths of about 40 mm. All eggs are diagenetically compressed and their original...... diameters are estimated at 45-50 mm. The eggshells are 0.3-0.5 mm thick, partly recrystallized, but widely still aragonitic. They consist of needle-like crystals that form individual shell units. A few pores are preserved between these shell units. This shell-structure allows assignment to chelonian eggs...... in the oofamily Testudoolithidae and the oogenus Testudolithus. The external morphology, microstructure and mineralogical composition of the eggshells show close resemblance to eggs of the extant tortoise Geochelone elephantopus. Together with a small association of turtle carapace fragments from the same...

  14. Reconciling Organisational Culture and External Quality Assurance in Higher Education

    Science.gov (United States)

    Naidoo, Dhaya

    2013-01-01

    Organisational culture and external quality assurance have both been presented as significant drivers of effectiveness, efficiency and excellence in higher education institutions. However, these assumptions have not been critically examined given the philosophical, conceptual and methodological contestations surrounding both constructs. A…

  15. Effect of Probiotic Preparation Enriched with Selenium on Qualitative Parameters of Table Eggs

    Directory of Open Access Journals (Sweden)

    Martin Mellen

    2014-05-01

    Full Text Available In this experiment the effects of the diet for laying hens supplemented with probiotic product with an organic form of selenium on egg weight, albumen quality, yolk quality and egg shell quality were studied. Isa Brown hens (n=90 were randomly divided at the age of 17 weeks into three groups (30 birds per group. Hens in all groups consumed the complete feed mixture ad libitum. In the control group water for drinking contained no additions. In the first experimental group probiotic product was added to the water, in the second experimental group the same probiotic preparation enriched with 0.8 to 1 mg of organic selenium per 1 g of the product was added to the water. The probiotic preparations were administered at the dose of 15 mg per 6 l of water daily, in both experimental groups. Monitored physical parameters of eggs: egg weight (g, specific egg weight (g/cm3​​, albumen weight (g, albumen height (mm, albumen index, Haugh units (HJ, yolk weight (g, yolk index, yolk color (°HLR, egg shell weight, egg shell specific weight (g/cm3, egg shell strength (N/cm2, the average eggshell thickness (µm. Experiment lasted 48 weeks. The results showed that egg weight was slightly higher in both experimental groups compared with the control group, differences between the groups were not statistically significant (P>0.05. The values ​​in the order of groups: 60.97 ± 4.97, 61.18 ± 5:00; 61.75 ± 5.89 (g ± SD. Was found insignificant impact of the add probiotic preparation and probiotic preparation enriched with selenium on the quality parameters of table eggs. Yolk index, albumen index, Haugh units and the average egg shell thickness were only slightly, statistically insignificant higher in the experimental groups (P> 0.05.

  16. Comparative performances of eggs and embryos of sea urchin (Paracentrotus lividus) in toxicity bioassays used for assessment of marine sediment quality.

    Science.gov (United States)

    Khosrovyan, A; Rodríguez-Romero, A; Salamanca, M J; Del Valls, T A; Riba, I; Serrano, F

    2013-05-15

    The potential toxicity of sediments from various ports was assessed by means of two different liquid-phase toxicity bioassays (acute and chronic) with embryos and eggs of sea urchin Paracentrotus lividus. Performances of embryos and eggs of P. lividus in these bioassays were compared for their interchangeable applicability in integrated sediment quality assessment. The obtained endpoints (percentages of normally developed plutei and fertilized eggs) were linked to physical and chemical properties of sediments and demonstrated dependence on sediment contamination. The endpoints in the two bioassays were strongly correlated and generally exhibited similar tendency throughout the samples. Therein, embryos demonstrated higher sensitivity to elutriate exposure, compared to eggs. It was concluded that these tests could be used interchangeably for testing toxicity of marine sediments. Preferential use of any of the bioassays can be determined by the discriminatory capacity of the test or vulnerability consideration of the test subject to the surrounding conditions. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. EFFECTS OF DIFFERENT SUPPLEMENTS INCLUSION OF THE ENRICHED DIET ON PRODUCTIVE PERFORMANCE AND EGG STRUCTURE

    Directory of Open Access Journals (Sweden)

    Natasha Trajan Gjorgovska

    2016-04-01

    Full Text Available Nutritional manipulation and genetic selection for egg size and production may lead to changes in egg components. This experiment was carried out to analyze the egg structure parameters of eggs produced by Hisex Brown laying hens fed with diet with different supplements inclusion. The intensity of egg production was significantly higher in the groups fed with enriched feed with iodine (90.00%, vitamin E (90.00% and selenium (91.98%, and significantly lower in the group fed with DHA inclusion feed (76.00% in respect to the control group (82.00%, confidence interval of 95%. Concerning diet supplemented with selenium and diet supplemented with vitamin E, the egg yolk weight was statistically different compared with the control group (confidence interval of 95%. The yolk weight averaged 1.80 g and 1.29 g more than yolk weight in the control eggs, respectively. The egg shell weight was statistically different in diet enriched with iodine compared with the control. The egg shell weight averaged 1.48 g less per egg for the eggs enriched with iodine with 17.45% less underweight than the control eggs. Enriched eggs offer consumer a variety of value-added options for their egg purchase. Although enriched eggs may provide consumers with a specific quality attribute or healthful ingredient, they do not appear to provide quality and value in a traditional sense as defined by the standards of quality and grade.

  18. Feed supplementation with red seaweeds, Chondrus crispus and Sarcodiotheca gaudichaudii, affects performance, egg quality, and gut microbiota of layer hens.

    Science.gov (United States)

    Kulshreshtha, Garima; Rathgeber, Bruce; Stratton, Glenn; Thomas, Nikhil; Evans, Franklin; Critchley, Alan; Hafting, Jeff; Prithiviraj, Balakrishnan

    2014-12-01

    The aim of this study was to evaluate the effect of the inclusion of red seaweed supplementation to standard poultry diets on production performance, egg quality, intestinal histology, and cecal short-chain fatty acids in Lohmann Brown Classic laying hens. A total of 160 birds were randomly assigned to 8 treatment groups. Control hens were fed a basal layer diet; positive control hens were fed a diet containing 2% inulin; and 6 treatment groups were fed a diet containing one of the following; 0.5, 1, or 2% Chondrus crispus (CC0.5, CC1, and CC2, respectively) and one of the same 3 levels of Sarcodiotheca gaudichaudii (SG0.5, SG1, and SG2, respectively). Dietary supplementation had no significant effect on the feed intake, BW, egg production, fecal moisture content, and blood serum profile of the birds. The feed conversion ratio per gram of egg was significantly more efficient (P = 0.001) for CC2 and SG2 treatments. Moreover, SG1 supplementation increased egg yolk weight (P = 0.0035) and birds with CC1 supplementation had higher egg weight (P = 0.0006). The SG2 and CC2 groups had greater (P Poultry Science Association Inc.

  19. Genetic Compatibility Underlies Benefits of Mate Choice in an External Fertilizer.

    Science.gov (United States)

    Aguirre, J David; Blows, Mark W; Marshall, Dustin J

    2016-05-01

    Mate choice is a common feature of sexually reproducing species. In sessile or sedentary external fertilizers, however, direct interactions between reproductive partners are minimal, and instead mate recognition and choice must occur at the level of gametes. It is common for some sperm and egg combinations to have higher fertilization success than others, but it remains unclear whether differences in fertilization reflect gamete-level mate choice (GMC) for paternal quality or parental compatibility. Here, we examine the mechanisms underlying GMC in an externally fertilizing ascidian. A manipulative mate-choice assay confirmed that offspring viability was greater in clutches where we allowed GMC than in clutches where we precluded GMC. A complementary quantitative genetic experiment then revealed that paternal quality effects were generally weaker than parental compatibility effects, particularly for the trait combination underlying the benefits of GMC. Overall, our data suggest that gametes that are more compatible at fertilization produce more viable offspring than gametes that are less compatible at fertilization. Therefore, although the regalia we typically associate with sexual selection are absent in external fertilizers, mechanisms that allow females to bias fertilization in favor of some males over others produce significant fitness benefits in organisms reproducing via the ancestral strategy.

  20. Egg quality and blood parameters of “Bianca di Saluzzo” and Isa Brown hens kept under free range conditions

    Directory of Open Access Journals (Sweden)

    Luca Doglione

    2010-01-01

    Full Text Available Egg quality traits and some blood parameters of Bianca di Saluzzo hen (BSh were compared to Isa Brown hen (IBh. Birds were reared in free range conditions. Weightofthewholeegg Weight of the whole egg was higher (P≤0.01 for IBh, due to its higher amount of albumen (P≤0.01 whereas BSh egg showed the highest weight and relative percentage of yolk (PP≤0.01. IBh and BSh egg chemical composition was similar.Totalredcellsdidnotshowstatisticaldifferencesbetweengroups,whiletotalleucocytes Total red cells did not show statistical differences between groups, while total leucocytes were lower (P≤0.05 forBShhens. Heterophyl/Lymphocyte(H/L ratioof BShwaslower (P for BSh hens. Heterophyl/Lymphocyte(H/L ratioof BShwaslower (P . Heterophyl/Lymphocyte(H/L ratioof BShwaslower (P Heterophyl/Lymphocyte (H/L ratio of BSh was lower (P (PP≤0.01 than IBh(0.33 IBh (0.33 (0.33 vs. 0.82.IBh 0.82. IBh IBh α-1 acid glycoprotein (AGPmeanconcentrationswereaffectedbytime: (AGP mean concentrations were affected by time: the values registered after 15 d (T1 and 2 months (T2 of observation were similar (345±132 and 279±58 μg/mlrespectively,whileAGPvalueregisteredafter4months(T3weresignificantlyhigher μg/ml respectively,whileAGPvalueregisteredafter4months(T3weresignificantlyhigher , while AGP value registered after 4 months (T3 were significantly higher (700±487 μg/ml;BShdidnotdisplayanyAGPstatisticalvariationovertime.IBhalbuminmeancon- μg/ml; BShdidnotdisplayanyAGPstatisticalvariationovertime.IBhalbuminmeancon- BSh did not display any AGP statistical variation over time. IBh albumin mean con- centration (1.62 g/dL was statistically lower than BSh values (1.89 g/dL; in both groups no effect of time of sampling was recorded. Acutephaseprotein(APPvaluesofBShsuggestedamoreadaptive Acute phase protein (APPvaluesofBShsuggestedamoreadaptive (APP values of BSh suggested a more adaptive attitude to free range conditions and appeared more constant over time. The present

  1. Feeding Diets Containing Different Forms of Duckweed on Productive Performance and Egg Quality of Ducks

    Directory of Open Access Journals (Sweden)

    B. Indarsih

    2012-08-01

    Full Text Available The present experiment was undertaken to study the feeding effect of diets containing different forms of duckweed for local ducks on their productive performance and egg quality or egg yolk pigmentation. A total of 90 birds of 24 wk old ducks were randomly divided into 18 experimental units of 2.0 x 1.0 m2 of cages. The experiment was assigned in a completely randomized design (3 treatments with 6 replicates, 5 birds each. There were 3 dietary treatments, namely P1= ducks fed a complete diet containing 20 % of dried duckweed and given in the form of dry-mash; P2= a complete diet in P1 but it was offered in wet form (slurry; and P3= ducks were offered basal diet in the form of dry-mash and fresh duckweed was offered separately ad libitum. Diets were formulated to have similar nutritional contents. Feed consumption, feed conversion ratio (FCR, and egg yolk pigmentation were measured. The result of the study showed that these three parameters were affected by the feeding different forms of duckweed. Feeding diet with fresh duckweed brought about the best pigmentation than did the dry one. Fresh duckweed offers a promise as a potential feedstuff for ducks and has a good implication in reducing feed processing cost.

  2. DIAGNOSIS OF EGG MARKETING CONDITION IN SINOP - MT

    Directory of Open Access Journals (Sweden)

    M. Ribeiro

    2015-10-01

    Full Text Available The eggs, when manipulated in inadequate sanitary conditions, are considered as ways for development and multiplication of spoilage and pathogenic microorganisms. Thus, the objective was to evaluate the microbiological quality of eggs marketed out at Sinop – MT, through the identification of Salmonella spp., count of aerobic mesophile bacteria and enterobacteria. We collected 12 eggs from each establishment, three supermarkets and three fairs. Were not identified Salmonella spp. in egg shells and internal content. Escherichia coli was isolated from the internal contents of eggs come from an street fair. In aerobic mesophilic counts were obtained from eggs supermarkets counts CFU/g/egg shell of 5,8 x 105, 1,21 x 105 e 4,7 x 103. In relation to the fairs, the counts were 7,2 x 104, 5,66 x 104 e 8,7 x 103. The absence of Salmonella spp. the evaluated eggs classifies them as fit for consumption for compliance with current legislation. However, the presence of E. coli and the values of aerobic mesophilic counts and Enterobacteriaceae highlight the need to establish measures to reduce bacterial contamination of shell eggs sold out at Sinop, MT.

  3. Parental Perceptions of Child Care Quality in Centre-Based and Home-Based Settings: Associations with External Quality Ratings

    Science.gov (United States)

    Lehrer, Joanne S.; Lemay, Lise; Bigras, Nathalie

    2015-01-01

    The current study examined how parental perceptions of child care quality were related to external quality ratings and considered how parental perceptions of quality varied according to child care context (home-based or centre-based settings). Parents of 179 4-year-old children who attended child care centres (n = 141) and home-based settings…

  4. MATHEMATICAL MODELING OF ELECTRO TECHNOLOGICAL OZONIZATION OF EGG STORES OF POULTRY FARMS

    OpenAIRE

    Voloshin A. P.

    2016-01-01

    Sanitization of eggs is an essential way to fight bacteria, fungi and other microorganisms. Hatchability of eggs and the safety of day-old chicks are dependent on the quality of eggs processing. Leading scientists of our country have proved high efficacy of ozone application for processing of hatching eggs. To obtain a positive result by this method of sanitizing hatching eggs ozone, it is necessary to create a uniform concentration of ozone around the egg store volume. Decrease in ozone conc...

  5. Egg shell quality and microstructure as affected by vitamin C, other feed additives and high environmental temperatures

    NARCIS (Netherlands)

    El-Boushy, A.R.

    1966-01-01

    The effects of climatic stress on egg formation (weight, shape, formation period, clutch size and blood calcium) and shell quality were estimated in White Leghorn and White Plymouth Rock hens. Constant environments were used with 85°F 75- 80% r.h., 75-77°F 50-60% r.h. and 55°F 50-60% r.h. as well as

  6. Advanced Oxidation Process sanitation of hatching eggs reduces Salmonella in broiler chicks

    Science.gov (United States)

    Reduction of Salmonella contamination of eggs is important in improving the microbial food safety of poultry and poultry products. Developing interventions to reduce Salmonella contamination of eggs is important to improving the microbial quality of eggs entering the hatchery. Previously, the hydr...

  7. Hen Egg as an Antioxidant Food Commodity: A Review.

    Science.gov (United States)

    Nimalaratne, Chamila; Wu, Jianping

    2015-09-24

    Intake of antioxidants through diet is known to be important in reducing oxidative damage in cells and improving human health. Although eggs are known for their exceptional, nutritional quality, they are not generally considered as antioxidant foods. This review aims to establish the importance of eggs as an antioxidant food by summarizing the current knowledge on egg-derived antioxidants. Eggs have various natural occurring compounds including the proteins ovalbumin, ovotransferrin and lysozyme in egg white, as well as phosvitin, carotenoids and free aromatic amino acids in egg yolk. Some lipophilic antioxidants such as vitamin E, carotenoids, selenium, iodine and others can be transferred from feed into egg yolk to produce antioxidant-enriched eggs. The bioactivity of egg antioxidants can be affected by food processing, storage and gastrointestinal digestion. Generally thermal processing methods can promote loss of antioxidant properties in eggs due to oxidation and degradation, whereas gastrointestinal digestion enhances the antioxidant properties, due to the formation of new antioxidants (free amino acids and peptides). In summary, in addition to its well-known nutritional contribution to our diet, this review emphasizes the role of eggs as an important antioxidant food.

  8. Effect of dietary vanadium and vitamin C on egg quality and antioxidant status in laying hens.

    Science.gov (United States)

    Wang, J P; He, K R; Ding, X M; Luo, Y H; Bai, S P; Zeng, Q F; Su, Z W; Xuan, Y; Zhang, K Y

    2016-06-01

    This study assessed the effect of dietary vanadium (V) and vitamin C (VC) on production performance, egg quality and antioxidant status in laying hens. A total of 360 laying hens (31-week-old) were randomly allotted into a 3 × 3 factorial arrangement treatments (four replicates and 10 chicks per replicate) with three levels of dietary V (0, 5 and 10 mg/kg) and three levels of vitamin C (0, 50 and 100 mg/kg) for 12 weeks. The effect of V and VC did not alter egg production, egg weight, average daily feed intake and feed conversion ratio during 1-12 week. Albumen height and Haugh unit value were linearly decreased (p effect of 100 mg/kg VC was observed to counteract (p effect in V-containing treatments during 1-12 week. Hens fed V-containing diet laid lighter (linear effect, p effect of V during 4, 8 and 12 week. The effect of VC alone and the interactive effect between VC and V were shown to increase serum (p effect and can mitigate the oxidative stress to some extent. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  9. The structure and ultrastructure of the egg capsules of stoneflies of the genus Isoperla (Insecta, Plecoptera, Perlodidae).

    Science.gov (United States)

    Michalik, Anna; Miliša, Marko; Michalik, Katarzyna; Rościszewska, Elżbieta

    2017-11-01

    The egg capsules of five systellognathan stoneflies species representing the genus Isoperla (Plecoptera, Perlodidae) have been investigated using light and electron microscopes (SEM and TEM). We consider the structural modifications of egg coverings (egg capsules, eggshells) like: a shape of a capsule, presence of structures fixing an egg to the substratum under water, intrachorionic aeropylar system facilitating gas exchange as a factor adapting an egg/embryo to the environment. The structures protecting eggs against desiccation during a female flight before oviposition into water as well as against the dangers of external mechanical injury caused by turbulences in rapid water currents of mountain streams are described and discussed. The ground plan of the egg capsule in arctoperlarian stoneflies is also discussed. © 2017 Wiley Periodicals, Inc.

  10. Quality electricity lines of external power systems electric traction DC

    Directory of Open Access Journals (Sweden)

    A.V. Petrov

    2012-08-01

    Full Text Available The results of studies that compare and analyze the numerical values of some key indicators quality electricity in the lines of the external power supply system the electric traction DC. As a supplement are additional and fundamental values of energy losses in them.

  11. The influence of Blue mussel (Mytilus edulis) and Starfish (Asterias rubens) meals on production performance, egg quality and apparent total tract digestibility of nutrients of laying hens

    DEFF Research Database (Denmark)

    Afrose, Sadia; Hammershøj, Marianne; Nørgaard, Jan Værum

    2016-01-01

    The aim of the study was to evaluate mussel meal and starfish meals as protein sources for organic layers by studying the effect on production performance, nutrient digestibility and egg quality. A total of 300 Hisex white laying hens (20-week old) were distributed randomly to 6 dietary treatment....... The egg weight was not different from the egg weights of control diets, but the inclusion of 4 g mussel meal resulted in a lower (P colour was lower (P ... 100 g feed can be used in diets as a high quality protein source for egg production. These meals can replace fish meal; however, an inclusion level higher than 8 g/100 g of mussel or starfish meal may result in a fishy smell of the eggs....

  12. Effect of γ-Aminobutyric Acid-producing Strain on Laying Performance, Egg Quality and Serum Enzyme Activity in Hy-Line Brown Hens under Heat Stress

    Directory of Open Access Journals (Sweden)

    Y. Z. Zhu

    2015-07-01

    Full Text Available Heat-stress remains a costly issue for animal production, especially for poultry as they lack sweat glands, and alleviating heat-stress is necessary for ensuring animal production in hot environment. A high γ-aminobutyric acid (GABA-producer Lactobacillus strain was used to investigate the effect of dietary GABA-producer on laying performance and egg quality in heat-stressed Hy-line brown hens. Hy-Line brown hens (n = 1,164 at 280 days of age were randomly divided into 4 groups based on the amount of freeze-dried GABA-producer added to the basal diet as follows: i 0 mg/kg, ii 25 mg/kg, iii 50 mg/kg, and iv 100 mg/kg. All hens were subjected to heat-stress treatment through maintaining the temperature and the relative humidity at 28.83±3.85°C and 37% to 53.9%, respectively. During the experiment, laying rate, egg weight and feed intake of hens were recorded daily. At the 30th and 60th day after the start of the experiment, biochemical parameters, enzyme activity and immune activity in serum were measured. Egg production, average egg weight, average daily feed intake, feed conversion ratio and percentage of speckled egg, soft shell egg and misshaped egg were significantly improved (p<0.05 by the increasing supplementation of the dietary GABA-producer. Shape index, eggshell thickness, strength and weight were increased linearly with increasing GABA-producer supplementation. The level of calcium, phosphorus, glucose, total protein and albumin in serum of the hens fed GABA-producing strain supplemented diet was significantly higher (p<0.05 than that of the hens fed the basal diet, whereas cholesterol level was decreased. Compared with the basal diet, GABA-producer strain supplementation increased serum level of glutathione peroxidase (p = 0.009 and superoxide dismutase. In conclusion, GABA-producer played an important role in alleviating heat-stress, the isolated GABA-producer strain might be a potential natural and safe probiotic to use to

  13. On the influence of storage duration on rheological properties of liquid egg products and response of eggs to impact loading - Japanese quail eggs

    Czech Academy of Sciences Publication Activity Database

    Kumbár, V.; Trnka, Jan; Nedomová, Š.; Buchar, J.

    2015-01-01

    Roč. 166, May (2015), s. 86-94 ISSN 0260-8774 Institutional support: RVO:61388998 Keywords : egg quality * japanese quail * haugh units * storage duration * non-Newtonian fluid * non-destructive impact * surface displacement Subject RIV: BI - Acoustics Impact factor: 3.199, year: 2015

  14. Free systems of rearing of chickens and layer hens: Quality of meat and eggs

    OpenAIRE

    Pavlovski, Zlatica; Škrbić, Zdenka; Lukić, Miloš

    2011-01-01

    Conventional broiler and egg production are basis of modern production of poultry meat and table eggs in the World and in our country. Standing requirements and demands for more and better in poultry production have induced continuous work on improvement of technologies of broiler rearing and production of table eggs. Improvements in technologies of production of meat and eggs depend on country's natural resources, environment conditions and, certainly, on consumer opinion and demands. In add...

  15. Effect of egg freshness on texture and baking characteristics of batter systems formulated using egg, flour and sugar.

    Science.gov (United States)

    Xing, Liting; Niu, Fuge; Su, Yujie; Yang, Yanjun

    2016-04-01

    The aim of this work was to evaluate the effects of egg freshness on baking properties and final qualities in batter systems. Batters were made with eggs of different freshness, and the properties of batter systems were studied through rheological analysis, rapid viscosity analysis (RVA), differential scanning calorimetry (DSC), batter density and expansion rate during the baking and cooling processes. Moreover, the qualities of final baked systems were investigated, including specific volume and texture profile analysis (TPA). The flow behavior of batters showed that the consistency index (K) decreased as the Haugh unit (HU) value decreased, while the flow behavior index (n) increased. Both the storage modulus (G') and loss modulus (G″) determined by mechanical spectra at 20 °C decreased with decreasing HU. RVA and DSC determinations revealed that lower-HU samples had a lower viscosity in the baking process and a shorter time for starch gelatinization and egg protein denaturation. Observation of the batter density revealed an increasing change, which was reflected by a decrease in the specific volume of final models. TPA showed significant differences in hardness and chewiness, but no significant differences in springiness and cohesiveness were found. The egg freshness affected the properties of batter systems. © 2015 Society of Chemical Industry.

  16. Introduction--the Socially Sustainable Egg Production project.

    Science.gov (United States)

    Swanson, J C; Mench, J A; Thompson, P B

    2011-01-01

    The social and political pressure to change egg production from conventional cage systems to alternative systems has been largely driven by the desire to provide more behavioral freedom for egg-laying hens. However, a change of this magnitude can affect other components of the production system and may result in unintended outcomes. To understand this issue, a Socially Sustainable Egg Production project was formed to 1) conduct a holistic and integrated systematic review of the current state of knowledge about various aspects of sustainable egg production, and 2) develop a coordinated grant proposal for future extramural funding based on the research priorities identified from the review. Expert study groups were formed to write evidence-based papers in 5 critical sustainability areas: hen health and welfare, economics, food safety and quality, public attitudes, and environmental impacts. These papers were presented as the PSA Emerging Issues Symposium on Social Sustainability of Egg Production at the 2010 Poultry Science Association meeting.

  17. Effects of different limestone particle sizes in the diet of broiler breeders post molting on their performance, egg quality, incubation results, and pre-starter performance of their progeny.

    Science.gov (United States)

    Bueno, I J M; Surek, D; Rocha, C; Schramm, V G; Muramatsu, K; Dahlke, F; Maiorka, A

    2016-04-01

    An experiment was conducted to test the hypothesis that a coarse limestone diet improves productivity, reproductive performance and the calcium utilization of molted broiler breeders. In total, 640 broiler breeder females, 73-week-old and sixty-four 27-week-old cockerels, Cobb 500, were evaluated during 10 weeks, according to a randomized block design composed of 4 treatments with 8 replicates each. Treatments consisted of diets with the inclusion of 100% fine limestone-fine PS (0.2 mm GMD-geometric mean diameter); PS1: 30% fine limestone+70% limestone with 1.0 mm GMD; PS2: 30% fine limestone+70% limestone with 2.0 mm GMD; and PS3: 30% fine limestone+70% limestone with 3.0 mm GMD. Calcium retention in the gizzard of the breeders, bone characteristics, and breeder performance, egg characteristics, eggshell quality, incubation performance, chick quality and yield, chick pre-starter live performance, and chick bone characteristics were determined. There was no significant difference (P>0.05) in the rate of lay, percentage of non-settable eggs, egg weight, egg shape index, egg specific gravity, eggshell weight, thickness, and percentage hatchability and egg weight loss of broiler breeders fed with diets with different limestone particle sizes. The chick quality and yield, chick pre-starter live performance, and chick bone characteristics were not affected (P>0.05) by any of the limestone particle sizes. It was concluded that live and reproductive performance parameters of broiler breeders post molting is not affected by limestone particle size in the feed. © 2016 Poultry Science Association Inc.

  18. Higher Education Quality: Perception Differences among Internal and External Stakeholders

    Science.gov (United States)

    Abidin, Munirul

    2015-01-01

    Conceptually, education quality of higher education can be determined by evaluation of their stakeholders's satisfaction level. The purpose of this study is to describe how students as external stakeholder and lecturers as internal stakeholder, perceived their satisfaction of learning experience in the university. This study was conducted in…

  19. Can a combination of average of normals and "real time" External Quality Assurance replace Internal Quality Control?

    Science.gov (United States)

    Badrick, Tony; Graham, Peter

    2018-03-28

    Internal Quality Control and External Quality Assurance are separate but related processes that have developed independently in laboratory medicine over many years. They have different sample frequencies, statistical interpretations and immediacy. Both processes have evolved absorbing new understandings of the concept of laboratory error, sample material matrix and assay capability. However, we do not believe at the coalface that either process has led to much improvement in patient outcomes recently. It is the increasing reliability and automation of analytical platforms along with improved stability of reagents that has reduced systematic and random error, which in turn has minimised the risk of running less frequent IQC. We suggest that it is time to rethink the role of both these processes and unite them into a single approach using an Average of Normals model supported by more frequent External Quality Assurance samples. This new paradigm may lead to less confusion for laboratory staff and quicker responses to and identification of out of control situations.

  20. Does egg competition occur in marine broadcast-spawners?

    Science.gov (United States)

    Marshall, D J; Evans, J P

    2005-09-01

    When the availability of sperm limits female reproductive success, competition for sperm, may be an important broker of sexual selection. This is because sperm limitation can increase the variance in female reproductive success, resulting in strong selection on females to compete for limited fertilization opportunities. Sperm limitation is probably common in broadcast-spawning marine invertebrates, making these excellent candidates for investigating scramble competition between broods of eggs and its consequences for female reproductive success. Here, we report our findings from a series of experiments that investigate egg competition in the sessile, broadcast-spawning polychaete Galeolaria caespitosa. We initially tested whether the order in which eggs encounter sperm affects their fertilization success at two ecologically relevant current regimes. We used a split-clutch-split--ejaculate technique to compare the fertilization success of eggs from individual females that had either first access (competition-free treatment) or second access (egg competition treatment) to a batch of sperm. We found that fertilization success depended on the order in which eggs accessed sperm; eggs that were assigned to the competition-free treatment exhibited significantly higher fertilization rates than those assigned to the egg competition treatment at both current speeds. In subsequent experiments we found that prior exposure of sperm to eggs significantly reduced both the quantity and quality of sperm available to fertilize a second clutch of eggs, resulting in reductions in fertilization success at high and low sperm concentrations. These findings suggest that female traits that increase the likelihood of sperm-egg interactions (e.g. egg size) will respond to selection imposed by egg competition.

  1. Factors affecting thermal resistance of Salmonella enterica serovar enteritidis ODA 99-30581-13 in shell egg contents and use of heat-ozone combinations for egg pasteurization.

    Science.gov (United States)

    Perry, Jennifer J; Yousef, Ahmed E

    2013-02-01

    Infection of laying hens with Salmonella enterica serovar Enteritidis leads to deposition of the pathogen into the albumen or yolk of forming eggs. Heat treatment can inactivate internalized Salmonella Enteritidis in shell eggs, but factors such as the nature and location of contamination may influence the efficacy of thermal treatments. In the current research, natural contamination was mimicked by introducing small inocula of Salmonella Enteritidis into different locations of shell eggs and incubating inoculated eggs. These pathogen-containing eggs were heated at 57°C for 40 min, and temperature within eggs was monitored at the locations of inocula. Comparison of inactivation at equivalent internal temperatures revealed similar levels of lethality regardless of inoculum location. Refrigeration between incubation and heat treatment did not increase thermal resistance of cells in albumen but decreased cell inactivation in yolk. Sequential application of heat and gaseous ozone allows for the development of a process capable of decontaminating shell eggs with minimal thermal treatment and impact on egg quality. Inoculated eggs were subjected to (i) an immersion heating process similar to that used in commercial pasteurization or (ii) immersion heating, at reduced duration, followed by vacuum (50.8 kPa) and treatment with ozone gas (maximum 160 g/m(3)) under pressure (∼187.5 kPa). All treatments tested produced greater than 5-log inactivation, which is required for "pasteurization" processes. Differences were observed in the visual quality of eggs depending on treatment parameters. Application of ozone subsequent to heating allows for a significant reduction in heating time without decreasing process lethality.

  2. Patterns of radiocesium contamination in eggs of free-ranging wood ducks

    International Nuclear Information System (INIS)

    Kennamer, R.A.; McCreedy, C.D.; Brisbin, I.L. Jr.

    1993-01-01

    Studies of biological consequences of nuclear industrial contaminants in the environment increase in importance as the potential for exposure of wildlife populations rises. Thus, we examined levels of radiocesium (cesium-137) contamination in wood duck eggs from a Department of Energy nuclear facility in South Carolina during 1990. Radiocesium levels in individual eggs from 5 locations on the site ranged from below detectable concentrations to a maximum of 2.21 Bq/g wet mass. Eggs from an abandoned reactor cooling reservoir contained the greatest burdens; mean egg concentrations there were more than an order of magnitude higher than those from other locations studied. Eggs from a second radionuclide releases, all contained similar low radiocesium levels that were, on average, below detection limits. Variation in radiocesium concentrations of eggs from the most contaminated cooling reservoir was partitioned into within- and among-clutch variation. Approximately 33% of the variation in radiocesium content occurred among eggs laid by the same female, and may have resulted because females fed in both contaminated and uncontaminated habitats during egg formation. Radiation dose rates to developing wood duck embryos, resulting from internal and external sources examined in this study, were below those expected to affect hatchability or any other aspect of the breeding biology of these birds. Wood ducks and their eggs may be useful as bioindicators of radiocesium uptake, but we caution that local foraging patterns could cause variation in contaminant deposition within clutches. 45 refs., 1 fig., 2 tabs

  3. Proteomics analysis of egg white proteins from different egg varieties.

    Science.gov (United States)

    Wang, Jiapei; Liang, Yue; Omana, Dileep A; Kav, Nat N V; Wu, Jianping

    2012-01-11

    The market of specialty eggs, such as omega-3-enriched eggs, organic eggs, and free-range eggs, is continuously growing. The nutritional composition of egg yolk can be manipulated by feed diet; however, it is not known if there is any difference in the composition of egg white proteins among different egg varieties. The purpose of the study was to compare the egg white proteins among six different egg varieties using proteomics analysis. Egg white proteins were analyzed using two-dimensional gel electrophoresis (2-DE), and 89 protein spots were subjected to LC-MS/MS. A total of 23 proteins, belonging to Gallus gallus , were identified from 72 detected protein spots. A quiescence-specific protein precursor in egg white was identified for the first time in this study. Significant differences in the abundant levels of 19 proteins (from 65 protein spots) were observed among six egg varieties. Four proteins, ovalbumin-related protein Y, cystatin, avidin, and albumin precursor, were not different among these six egg varieties. These findings suggest that the abundance, but not the composition, of egg white proteins varied among the egg varieties.

  4. EGG QUALITY AND HATCHABILITY OF In situ - REARED KEDU AND CEMANI HENS FED DIET OF FARMER FORMULATION SUPPLEMENTED WITH VITAMIN E

    Directory of Open Access Journals (Sweden)

    H.I. Wahyuni

    2014-10-01

    Full Text Available The aim of study was to evaluate the effect of dietary vitamin E supplementation in Kedu andCemani hens reraed in situ and given farmer-formulated diet on egg performances. A total of 120 femaleand 24 male birds were equally divided into two groups of Kedu and Cemani, with average body weightof 1890+216.79 and 1830+396.23 g, respectively. Basal ration was based on the diet formulated by thefarmer (R1 consisting of corn (30%, rice bran (50%, protein concentrate (15%, and premix (5%.Dietary treatments evaluated were R1 (without additional vitamin E, and the other three were theinclusion of DL α-tocopheryl acetate into the R1 diet up to 2 (R2, 4 (R3 and 6 IU (R4. The experimentwas arranged in a Randomized Block Design with 4 treatments, and 2 different groups of hen (Kedu andCemani were assigned as block. Parameters observed were feed consumption, hen day production, feedconversion ratio, egg quality, fertility, and hatchability. The result showed that vitamin Esupplementation did not affect whatever parameters, except egg fertility. Egg hatchability between Keduand Cemani hens was significantly different (p<0.05. Trend comparison test indicated significantlylinear (p<0.05 for fertility and hatchability. In conclusion, vitamin E supplementation up to 6 IUincreased linearly egg fertility and hatchability of about 5-8%, and egg hatchability of Kedu hens washigher (11% than that of Cemani.

  5. Genetic characterization of egg weight, egg production and age at first egg in quails

    NARCIS (Netherlands)

    Marubayashi Hidalgo, A.; Martins, E.N.; Santos, A.L.; Quadros, T.C.O.; Ton, A.P.S.; Teixeira, R.

    2011-01-01

    The objective of this research was to estimate genetic parameters for the traits egg weight, egg production in 189 days and age at first egg in three laying quails and one meat line of quails. Data was analyzed by Bayesian procedures using Gibbs sampling. The heritability estimates for egg weight,

  6. Immunologic changes in children with egg allergy ingesting extensively heated egg.

    Science.gov (United States)

    Lemon-Mulé, Heather; Sampson, Hugh A; Sicherer, Scott H; Shreffler, Wayne G; Noone, Sally; Nowak-Wegrzyn, Anna

    2008-11-01

    Prior studies have suggested that heated egg might be tolerated by some children with egg allergy. We sought to confirm tolerance of heated egg in a subset of children with egg allergy, to evaluate clinical and immunologic predictors of heated egg tolerance, to characterize immunologic changes associated with continued ingestion of heated egg, and to determine whether a diet incorporating heated egg is well tolerated. Subjects with documented IgE-mediated egg allergy underwent physician-supervised oral food challenges to extensively heated egg (in the form of a muffin and a waffle), with tolerant subjects also undergoing regular egg challenges (in a form of scrambled egg or French toast). Heated egg-tolerant subjects incorporated heated egg into their diets. Skin prick test wheal diameters and egg white, ovalbumin, and ovomucoid IgE levels, as well as ovalbumin and ovomucoid IgG4 levels, were measured at baseline for all subjects and at 3, 6, and 12 months for those tolerant of heated egg. Sixty-four of 117 subjects tolerated heated egg, 23 tolerated regular egg, and 27 reacted to heated egg. Heated egg-reactive subjects had larger skin test wheals and greater egg white-specific, ovalbumin-specific, and ovomucoid-specific IgE levels compared with heated egg- and egg-tolerant subjects. Continued ingestion of heated egg was associated with decreased skin test wheal diameters and ovalbumin-specific IgE levels and increased ovalbumin-specific and ovomucoid-specific IgG4 levels. The majority of subjects with egg allergy were tolerant of heated egg. Continued ingestion of heated egg was well tolerated and associated with immunologic changes that paralleled the changes observed with the development of clinical tolerance to regular egg.

  7. Deposition of carotenoids in egg yolk by short-term supplement of coloured carrot (Daucus carota) varieties as forage material for egg-laying hens.

    Science.gov (United States)

    Hammershøj, Marianne; Kidmose, Ulla; Steenfeldt, Sanna

    2010-05-01

    Supplying egg-laying hens with different forage materials may influence egg production and quality. The aim of this study was to examine the short-term effects of standard feed plus 70 g day(-1) per hen of three coloured carrot varieties (orange, yellow and purple) as forage material in comparison with a standard feed control on egg production, egg yolk colour and deposition of carotenoids in the yolk. Carrot supplementation reduced feed intakes significantly, but not on a dry matter basis. Orange carrot treatment significantly reduced egg mass production, whereas yellow and purple carrot treatments did not differ from the control. Egg and yolk weights of all carrot-supplemented treatments were significantly lower than those of the control, but yolk percentages were similar. Yolk redness increased significantly in the order control 1.5-fold) and beta-carotene (>100-fold) compared with the control. Supplementing the feed of egg-laying hens with coloured carrots efficiently increased yolk colour parameters and carotenoid contents, which gives opportunities for improved nutritional value of eggs from forage material-supplemented hens.

  8. External quality audits in radiotherapy in Poland

    International Nuclear Information System (INIS)

    Bulski, W.; Rostkowska, J.; Kania, M.; Gwiazdowska, B.

    2002-01-01

    The Secondary Standard Dosimetry Laboratory (SSDL) of the Medical Physics Department of the Centre of Oncology in Warsaw is a continuation of the Radiation Measurements Laboratory created in 1937, following the suggestions of Marie Curie, the founder of the Institute. The present SSDL is a member of the WHO/IAEA international network and is periodically audited by the International Atomic Energy Agency. The SSDL is in charge of the calibration of all radiotherapy dosimeters in Poland, and it also co-ordinates all activities carried out in radiotherapy quality assurance programmes nation-wide. The External Audit Group (EAG) was set-up according to the recommendations of the IAEA, as a part of the SSDL. The EAG is in charge of the management of the project and organization of the TLD measurements. The SSDL takes the responsibilities of the metrological aspects of the programme. The results of the efforts, aimed at the development of a quality audit programme and methodology in radiotherapy, are presented

  9. 9 CFR 590.905 - Importation of restricted eggs or eggs containing more restricted eggs than permitted in the...

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Importation of restricted eggs or eggs containing more restricted eggs than permitted in the official standards for U.S. Consumer Grade B. 590.905... EGG PRODUCTS INSPECTION INSPECTION OF EGGS AND EGG PRODUCTS (EGG PRODUCTS INSPECTION ACT) Imports...

  10. Effect of using garlic or curcumin or parsley on the growth performance, meat and egg quality of Japanese quail

    International Nuclear Information System (INIS)

    El-Faramawy, A.A.; Fahmy, M.O.; Wakwak, M.M.

    2006-01-01

    Two grams of powdered garlic or curcumin or parsley were added separately to each kg of the diet of unsexed Japanese quail aged one day old in an attempt to evaluate growth performance, serum cholesterol, triiodothyronine hormone, small intestine thickness and caecal microbial count to improve meat quality and egg production. The quails were hold in equal 4 groups; each group contained three replicates of 30 birds. Birds were watered and fed ad libitum for 7 weeks then 69 chicks from each group (6 males and 17 females for each replicate) were transferred into laying cages for another 6 weeks while 3 birds from each replicate were slaughtered for serum cholesterol, triiodothyronine, small intestine thickness, microbial count and carcass fatty acid determination. The data revealed that addition of each of the tested herb supplements caused significant increase in body weight, while there was non-significant changes in serum cholesterol, T3, small intestine thickness and total bacterial count. In addition, there was significant increase in unsaturated fatty acids and significant decrease in saturated fatty acids of carcass. The three additives caused remarkable increases in egg production and hatch ability %, besides augmenting the fertility % and number of ovarian follicles. It may be concluded that addition of 2g garlic or curcumin or parsley powder to each kg of the diet of Japanese quail favored consumer expectation in relation to meat quality and egg production and prolonged feed preservation

  11. Effect of γ-Aminobutyric Acid-producing Lactobacillus Strain on Laying Performance, Egg Quality and Serum Enzyme Activity in Hy-Line Brown Hens under Heat Stress.

    Science.gov (United States)

    Zhu, Y Z; Cheng, J L; Ren, M; Yin, L; Piao, X S

    2015-07-01

    Heat-stress remains a costly issue for animal production, especially for poultry as they lack sweat glands, and alleviating heat-stress is necessary for ensuring animal production in hot environment. A high γ-aminobutyric acid (GABA)-producer Lactobacillus strain was used to investigate the effect of dietary GABA-producer on laying performance and egg quality in heat-stressed Hy-line brown hens. Hy-Line brown hens (n = 1,164) at 280 days of age were randomly divided into 4 groups based on the amount of freeze-dried GABA-producer added to the basal diet as follows: i) 0 mg/kg, ii) 25 mg/kg, iii) 50 mg/kg, and iv) 100 mg/kg. All hens were subjected to heat-stress treatment through maintaining the temperature and the relative humidity at 28.83±3.85°C and 37% to 53.9%, respectively. During the experiment, laying rate, egg weight and feed intake of hens were recorded daily. At the 30th and 60th day after the start of the experiment, biochemical parameters, enzyme activity and immune activity in serum were measured. Egg production, average egg weight, average daily feed intake, feed conversion ratio and percentage of speckled egg, soft shell egg and misshaped egg were significantly improved (pGABA-producer. Shape index, eggshell thickness, strength and weight were increased linearly with increasing GABA-producer supplementation. The level of calcium, phosphorus, glucose, total protein and albumin in serum of the hens fed GABA-producing strain supplemented diet was significantly higher (plevel was decreased. Compared with the basal diet, GABA-producer strain supplementation increased serum level of glutathione peroxidase (p = 0.009) and superoxide dismutase. In conclusion, GABA-producer played an important role in alleviating heat-stress, the isolated GABA-producer strain might be a potential natural and safe probiotic to use to improve laying performance and egg quality in heat-stressed hens.

  12. Patterns and Drivers of Egg Pigment Intensity and Colour Diversity in the Ocean: A Meta-Analysis of Phylum Echinodermata.

    Science.gov (United States)

    Montgomery, E M; Hamel, J-F; Mercier, A

    Egg pigmentation is proposed to serve numerous ecological, physiological, and adaptive functions in egg-laying animals. Despite the predominance and taxonomic diversity of egg layers, syntheses reviewing the putative functions and drivers of egg pigmentation have been relatively narrow in scope, centring almost exclusively on birds. Nonvertebrate and aquatic species are essentially overlooked, yet many of them produce maternally provisioned eggs in strikingly varied colours, from pale yellow to bright red or green. We explore the ways in which these colour patterns correlate with behavioural, morphological, geographic and phylogenetic variables in extant classes of Echinodermata, a phylum that has close phylogenetic ties with chordates and representatives in nearly all marine environments. Results of multivariate analyses show that intensely pigmented eggs are characteristic of pelagic or external development whereas pale eggs are commonly brooded internally. Of the five egg colours catalogued, orange and yellow are the most common. Yellow eggs are a primitive character, associated with all types of development (predominant in internal brooders), whereas green eggs are always pelagic, occur in the most derived orders of each class and are restricted to the Indo-Pacific Ocean. Orange eggs are geographically ubiquitous and may represent a 'universal' egg pigment that functions well under a diversity of environmental conditions. Finally, green occurs chiefly in the classes Holothuroidea and Ophiuroidea, orange in Asteroidea, yellow in Echinoidea, and brown in Holothuroidea. By examining an unprecedented combination of egg colours/intensities and reproductive strategies, this phylum-wide study sheds new light on the role and drivers of egg pigmentation, drawing parallels with theories developed from the study of more derived vertebrate taxa. The primary use of pigments (of any colour) to protect externally developing eggs from oxidative damage and predation is

  13. Bactericidal paper trays doped with silver nanoparticles for egg ...

    Indian Academy of Sciences (India)

    the AgNPs-deposited paper egg trays improved the shelf-life of the eggs by more than 14 days ... In this work, we developed a new method to prepare anti- ... on an electronic balance (Sartorius). ..... significant changes with respect to physical quality param- ... Due to possible human health effects from silver exposure,.

  14. Evaluasi Pengaruh Penggunaan Bahan Pengganti Telur (Egg Replacer) Pada Pembuatan Cake

    OpenAIRE

    Dewi, Nova Sarifah

    2015-01-01

    Nowdays many people are allergic to egg either the white or the egg yolk, that is why the research way conducted. The research was aimed to evaluate the functional characteristics and sensory quality of cakes made of soy protein isolate, whey protein isolate, corn starch, guar gum, xanthan gum and whole egg powder. The research had been performed using a non factorial completely randomized design with 7 levels and 3 repetition. The egg replacer composition were E1 = whey protein isolate (18,...

  15. Egg Allergy

    Science.gov (United States)

    ... Staying Safe Videos for Educators Search English Español Egg Allergy KidsHealth / For Teens / Egg Allergy What's in ... but it's worth it. What Happens With an Egg Allergy? Eggs aren't bad. But when you' ...

  16. Investigation on the effects of dietary protein reduction with constant ratio of digestible sulfur amino acids and threonine to lysine on performance, egg quality and protein retention in two strains of laying hens

    Directory of Open Access Journals (Sweden)

    Farhad Foroudi

    2013-01-01

    Full Text Available An experiment was conducted to determine the possibility of using various levels of crude protein (CP by providing laying hens with constant levels of digestible sulfur amino acid, threonine and lysine to improve performance and egg quality. The experiment was conducted as a completely randomized block design in a factorial arrangement (4 × 2 with 8 replicates of 10 hens in each. Factors included 4 levels of CP (18.5%, 17.5%, 16.5% and 15.5% and 2 strains (LSL and Hy-Line W-36 of laying hens. Hens were fed experimental diets from 25 to 33 weeks of age. Production performance was measured for eight weeks and egg quality characteristics were determined at 29 and 33 weeks of age. Protein reduction decreased egg weight, egg mass and hen body weight linearly (P≤0.01. Egg production was not affected by protein reduction but feed efficiency, and average daily feed intake increased significantly (P≤0.01. Lohmann Selected Leghorn laying hens showed significantly higher egg production, egg weight, egg mass, weight gain, feed efficiency and feed intake compared to the W-36 laying hens (P≤0.01. Shell thickness increased linearly as protein levels decreased (P≤0.05. There were significant differences between two strains on the egg quality characteristics (P≤0.01. Significant (P≤0.05 CP × strain interactions were observed for hen weight, albumen height, Haugh units, yolk and shell percentage. Based on the results of this experiment, a reduction in dietary protein level (from 18.5% to 15.5%, without any alteration in digestible TSAA and Thr: Lys ratio, led to inferior egg mass and feed conversion ratio during the peak production period.

  17. Response of laying hens to feeding low-protein amino acid-supplemented diets under high ambient temperature: performance, egg quality, leukocyte profile, blood lipids, and excreta pH

    Science.gov (United States)

    Torki, Mehran; Mohebbifar, Ahmad; Ghasemi, Hossein Ali; Zardast, Afshin

    2015-05-01

    An experiment was conducted to determine whether, by using a low-protein amino acid-supplemented diet, the health status, stress response, and excreta quality could be improved without affecting the productive performance of heat-stressed laying hens. The requirements for egg production, egg mass, and feed conversion ratio were also estimated using second-order equations and broken-line regression. A total of 150 Lohmann Selected Leghorn (LSL-Lite) hens were divided randomly into five groups of 30 with five replicates of six hens. The hens were raised for an 8-week period (52 to 60 weeks) in wire cages situated in high ambient temperature in an open-sided housing system. The five experimental diets (ME; 2,720 kcal/kg) varied according to five crude protein (CP) levels: normal-CP diet (control, 16.5 % CP) and low-CP diets containing 15.0, 13.5, 12.0, or 10.5 % CP. All experimental diets were supplemented with crystalline amino acids at the levels sufficient to meet their requirements. The results showed that under high temperature conditions, all productive performance and egg quality parameters in the birds fed with 15.0, 13.5, and 12.0 % CP diets were similar to those of birds fed with control diet (16.5 % CP), whereas feeding 10.5 % CP diet significantly decreased egg production and egg mass. Estimations of requirements were of 13.93 and 12.77 % CP for egg production, 14.62 and 13.22 % CP for egg mass, and 12.93 and 12.26 % CP for feed conversion ratio using quadratic and broken-line models, respectively. Egg yolk color index, blood triglyceride level, and excreta acidity were also significantly higher in birds fed with 12.0 and 10.5 % CP diets compared with those of control birds. The heterophil to lymphocyte ratio, as a stress indicator, was significantly decreased by 15.0, 13.5, and 12 % CP diets. On the basis of our findings, reducing dietary CP from 16.5 to 12.0 % and supplementing the diets with the essential amino acids showed merit for improving the

  18. Naturally Occurring Egg Drop Syndrome Infection in Turkeys

    Directory of Open Access Journals (Sweden)

    Z. Biđin

    2007-01-01

    Full Text Available A decrease in the egg quality, production, fertility and hatchability without serious clinical signs of illness was recorded in turkey fl ocks in Croatia at the beginning of 2002. It was assumed that the egg drop syndrome virus might be one of the etiological agents responsible for the abnormalities in the egg production. The systematic serological monitoring, using a haemagglutination inhibition test, showed that the antibodies to the egg drop syndrome virus existed in 94.4 and 55.1% of the sera analysed in 2002 and 2003, respectively. The haemagglutination inhibition titres ranged from 16 to 128. The sera samples were randomly collected from 11 - to 46-week-old hens from the affected fl ocks. The serological evidence of the egg drop syndrome virus infection was confirmed by detection of the presence of the virus genome in the turkey sera by the polymerase chain reaction. Vaccination of the 18- and 25-week-old turkey hens against the egg drop syndrome virus started in March 2003. After this period, the presence of antibodies to the egg drop syndrome virus (the haemagglutination inhibition titres between 16 and 256 was found in 96.7% of the analysed sera, while the egg production reached normal or higher values for the Nicholas hybrid line of turkeys.

  19. [THE VIRTUAL CYTOLOGIC SLIDES FOR EXTERNAL EVALUATION OF QUALITY OF IMPLEMENTATION OF CYTOLOGIC ANALYSES IN CLINICAL DIAGNOSTIC LABORATORIES: POSSIBILITIES AND PERSPECTIVES].

    Science.gov (United States)

    Djangirova, T V; Shabalova, I P; Pronichev, A N; Polyakov, E V

    2015-08-01

    The article considers application of technology of analysis of cytological slides in external quality control of clinical diagnostic laboratories. The advantages of virtual slides are demonstrated against other applied technologies of external evaluation of quality i.e. slide plate and digital micro-photography. The conditions of formation of virtual slides for external evaluation of quality of clinical diagnostic laboratories. The technology of their application is described. The success of practical application of considered technology in the Federal system of external evaluation of quality is emphasized.

  20. Using institutional theory to analyse hospital responses to external demands for finance and quality in five European countries

    Science.gov (United States)

    Mendel, Peter; Nunes, Francisco; Wiig, Siri; van den Bovenkamp, Hester; Karltun, Anette; Robert, Glenn; Anderson, Janet; Vincent, Charles; Fulop, Naomi

    2015-01-01

    Objectives Given the impact of the global economic crisis, delivering better health care with limited finance grows more challenging. Through the lens of institutional theory, this paper explores pressures experienced by hospital leaders to improve quality and constrain spending, focusing on how they respond to these often competing demands. Methods An in-depth, multilevel analysis of health care quality policies and practices in five European countries including longitudinal case studies in a purposive sample of ten hospitals. Results How hospitals responded to the financial and quality challenges was dependent upon three factors: the coherence of demands from external institutions; managerial competence to align external demands with an overall quality improvement strategy, and managerial stability. Hospital leaders used diverse strategies and practices to manage conflicting external pressures. Conclusions The development of hospital leaders’ skills in translating external requirements into implementation plans with internal support is a complex, but crucial, task, if quality is to remain a priority during times of austerity. Increasing quality improvement skills within a hospital, developing a culture where quality improvement becomes embedded and linking cost reduction measures to improving care are all required. PMID:26683885

  1. Using institutional theory to analyse hospital responses to external demands for finance and quality in five European countries.

    Science.gov (United States)

    Burnett, Susan; Mendel, Peter; Nunes, Francisco; Wiig, Siri; van den Bovenkamp, Hester; Karltun, Anette; Robert, Glenn; Anderson, Janet; Vincent, Charles; Fulop, Naomi

    2016-04-01

    Given the impact of the global economic crisis, delivering better health care with limited finance grows more challenging. Through the lens of institutional theory, this paper explores pressures experienced by hospital leaders to improve quality and constrain spending, focusing on how they respond to these often competing demands. An in-depth, multilevel analysis of health care quality policies and practices in five European countries including longitudinal case studies in a purposive sample of ten hospitals. How hospitals responded to the financial and quality challenges was dependent upon three factors: the coherence of demands from external institutions; managerial competence to align external demands with an overall quality improvement strategy, and managerial stability. Hospital leaders used diverse strategies and practices to manage conflicting external pressures. The development of hospital leaders' skills in translating external requirements into implementation plans with internal support is a complex, but crucial, task, if quality is to remain a priority during times of austerity. Increasing quality improvement skills within a hospital, developing a culture where quality improvement becomes embedded and linking cost reduction measures to improving care are all required. © The Author(s) 2015.

  2. Study on mixis potential of rotifer resting eggs ( Brachionus plicatilis) with different collection times and different preservation periods

    Science.gov (United States)

    Zhou, Li; Zheng, Yan; Xiang, Jian-Hai

    2001-09-01

    The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985 1998. Several clones derived from each batch of resting eggs were cultured under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed.

  3. Effect of length of lay of Nigeria indigenous chickens on their egg ...

    African Journals Online (AJOL)

    Three hundred and thirty eggs collected from the second to the seventh month of production, from a flock of the Nigeria indigenous (NI) chickens, were analysed to determine the effect of the length of lay on egg composition and shell quality. The weights of the whole egg and its components as well as shell thickness were ...

  4. The WHO AFRO external quality assessment programme (EQAP: Linking laboratory networks through EQA programmes

    Directory of Open Access Journals (Sweden)

    Debrah I. Boeras

    2016-10-01

    Full Text Available External Quality Assessment (EQA surveys performed by the World Health Organization Regional Office for Africa (WHO AFRO revealed the need for the strengthening of publichealth microbiology laboratories, particularly for testing of epidemic-prone diseases in theAfrican Region. These surveys revealed common issues such as supply chain managementskilled personnel, logistical support and overall lack of quality standards. For sustainableimprovements to health systems as well as global health security, deficiencies identified needto be actively corrected through robust quality assurance programmes and implementation oflaboratory quality management systems. Given all the pathogens of public health importance, an external quality assessment programmewith a focus on vaccine-preventable diseases and emerging and re-emerging dangerouspathogens is important, and should not be stand-alone, but integrated within laboratorynetworks as seen in polio, measles, yellow fever and rubella. In 2015, WHO AFRO collaborated with the US Centers for Disease Control and Preventionthe London School of Hygiene & Tropical Medicine and partners in a series of consultationswith countries and national and regional EQA providers for the development of qualityassurance models to support HIV point-of-care testing and monitoring. These consultationsrevealed similar challenges as seen in the WHO AFRO surveys. WHO AFRO brought forthits experience in implementing quality standards for health programmes, and also openeddiscussions on how lessons learned through such established programmes can be utilised tosupporting and strengthening the introduction of early infant diagnosis of HIV and viralload point-of-care testing. An optimised external quality assessment programme will impact the ability of countries tomeet core capacities, providing improved quality management systems, improving theconfidence of diagnostic network services in Africa, and including capacities to detect eventsof

  5. 7 CFR 57.905 - Importation of restricted eggs or eggs containing more restricted eggs than permitted in the...

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Importation of restricted eggs or eggs containing more restricted eggs than permitted in the official standards for U.S. Consumer Grade B. 57.905 Section 57.905... AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS INSPECTION ACT (CONTINUED) INSPECTION OF EGGS (EGG...

  6. Factors Affecting Microbial Contamination of Market Eggs: A Review

    Directory of Open Access Journals (Sweden)

    Svobodová J.

    2015-01-01

    Full Text Available The aim of the review was to analyze the ways of microbial contamination, the protective mechanism of egg, and factors that affect the quantity of contamination and microbial penetration. Eggs can be contaminated during their formation in the infected reproductive organs of hens or after laying, when eggs are exposed to contaminated environment. The eggs are equipped against microbial contamination by several protective mechanisms comprising the presence of cuticle, eggshell, eggshell membranes, occurrence of some antibacterial proteins, and high pH value of albumen. There are several factors that affect the quantity of microbial contamination and penetration such as species of bacteria, the amount of microorganisms, storage conditions, quality of eggshell or number of pores.

  7. Public health investigations of Salmonella Enteritidis in catering raw shell eggs, 2002-2004.

    Science.gov (United States)

    Little, C L; Surman-Lee, S; Greenwood, M; Bolton, F J; Elson, R; Mitchell, R T; Nichols, G N; Sagoo, S K; Threlfall, E J; Ward, L R; Gillespie, I A; O'Brien, S

    2007-06-01

    In response to a dramatic change in the epidemiology of Salmonella Enteritidis in England and Wales thought to be associated with raw shell eggs, the Health Protection Agency initiated public health investigations to establish the incidence of Salmonella contamination and origin of eggs used by catering premises implicated in outbreaks of Salm. Enteritidis. Between October 2002 and November 2004, 16 971 eggs were sampled and Salmonella were recovered from 3.4%. Salmonella was isolated from 5.5% and 6.3% of Spanish and eggs of unknown origin, respectively, used in catering premises linked to outbreaks, a level significantly higher than that (1.1%) found in nonLion Quality UK eggs sampled. The small sample of UK Lion Quality eggs tested (reflecting their lack of use in premises visited) did not contain Salmonella. Several phage types of Salm. Enteritidis other than phage type 4 (PT 4) were identified with nonUK eggs. Eggs from Spain were implicated as a major source of infection. Eggs were contaminated more frequently with Salmonella when shells were dirty and/or cracked, and stored at above 8 degrees C. The use of Spanish eggs by the catering sector has been identified as a consistent significant factor in many of the outbreaks caused by Salm. Enteritidis nonPT4 in England and Wales during 2002-2004. Advice to caterers and hospitals that raw shell eggs should not be used in food that will either not be cooked or only lightly cooked should be reinforced.

  8. The WHO AFRO external quality assessment programme (EQAP): Linking laboratory networks through EQA programmes.

    Science.gov (United States)

    Boeras, Debrah I; Peeling, Rosanna W; Onyebujoh, Philip; Yahaya, Ali A; Gumede-Moeletsi, Hieronyma N; Ndihokubwayo, Jean B

    2016-01-01

    External Quality Assessment (EQA) surveys performed by the World Health Organization Regional Office for Africa (WHO AFRO) revealed the need for the strengthening of public health microbiology laboratories, particularly for testing of epidemic-prone diseases in the African Region. These surveys revealed common issues such as supply chain management, skilled personnel, logistical support and overall lack of quality standards. For sustainable improvements to health systems as well as global health security, deficiencies identified need to be actively corrected through robust quality assurance programmes and implementation of laboratory quality management systems. Given all the pathogens of public health importance, an external quality assessment programme with a focus on vaccine-preventable diseases and emerging and re-emerging dangerous pathogens is important, and should not be stand-alone, but integrated within laboratory networks as seen in polio, measles, yellow fever and rubella. In 2015, WHO AFRO collaborated with the US Centers for Disease Control and Prevention, the London School of Hygiene & Tropical Medicine and partners in a series of consultations with countries and national and regional EQA providers for the development of quality assurance models to support HIV point-of-care testing and monitoring. These consultations revealed similar challenges as seen in the WHO AFRO surveys. WHO AFRO brought forth its experience in implementing quality standards for health programmes, and also opened discussions on how lessons learned through such established programmes can be utilised to supporting and strengthening the introduction of early infant diagnosis of HIV and viral load point-of-care testing. An optimised external quality assessment programme will impact the ability of countries to meet core capacities, providing improved quality management systems, improving the confidence of diagnostic network services in Africa, and including capacities to detect events

  9. Unravelling anisogamy: egg size and ejaculate size mediate selection on morphology in free-swimming sperm.

    Science.gov (United States)

    Monro, Keyne; Marshall, Dustin J

    2016-07-13

    Gamete dimorphism (anisogamy) defines the sexes in most multicellular organisms. Theoretical explanations for its maintenance usually emphasize the size-related selection pressures of sperm competition and zygote survival, assuming that fertilization of all eggs precludes selection for phenotypes that enhance fertility. In external fertilizers, however, fertilization is often incomplete due to sperm limitation, and the risk of polyspermy weakens the advantage of high sperm numbers that is predicted to limit sperm size, allowing alternative selection pressures to target free-swimming sperm. We asked whether egg size and ejaculate size mediate selection on the free-swimming sperm of Galeolaria caespitosa, a marine tubeworm with external fertilization, by comparing relationships between sperm morphology and male fertility across manipulations of egg size and sperm density. Our results suggest that selection pressures exerted by these factors may aid the maintenance of anisogamy in external fertilizers by limiting the adaptive value of larger sperm in the absence of competition. In doing so, our study offers a more complete explanation for the stability of anisogamy across the range of sperm environments typical of this mating system and identifies new potential for the sexes to coevolve via mutual selection pressures exerted by gametes at fertilization. © 2016 The Author(s).

  10. Dealing with multicollinearity in predicting egg components from egg weight and egg dimension

    Directory of Open Access Journals (Sweden)

    Tarek M. Shafey

    2014-10-01

    Full Text Available Measurements of 174 eggs from meat-type breeder flock (Ross at 36 weeks of age were used to study the problem of multicollinearity (MC instability in the estimation of egg components of yolk weight (YKWT, albumen weight (ALBWT and eggshell weight (SHWT. Egg weight (EGWT, egg shape index (ESI=egg width (EGWD*100/egg length (EGL and their interaction (EGWTESI were used in the context of un-centred vs centred data and principal components regression (PCR models. The pairwise phenotypic correlations, variance inflation factor (VIF, eigenvalues, condition index (CI, and variance proportions were examined. Egg weight had positive correlations with EGWD and EGL (r=0.56 and 0.50, respectively; P<0.0001 and EGL had a negative correlation with ESI (r=-0.79; P<0.0001. The highest correlation was observed between EGWT and ALBWT (r=0.94; P<0.0001, while the lowest was between EGWD and SHWT (r=0.33; P<0.0001. Multicollinearity problems were found in EGWT, ESI and their interaction as shown by VIF (>10, eigenvalues (near zero, CI (>30 and high corresponding proportions of variance of EGWT, ESI and EGWTESI with respect to EGWTESI. Results from this study suggest that mean centring and PCR were appropriate to overcome the MC instability in the estimation of egg components from EGWT and ESI. These methods improved the meaning of intercept values and produced much lower standard error values for regression coefficients than those from un-centred data.

  11. [Analysis of the results of the SEIMC External Quality Control Program. Year 2012].

    Science.gov (United States)

    de Gopegui Bordes, Enrique Ruiz; Guna Serrano, M del Remedio; Orta Mira, Nieves; Ovies, María Rosario; Poveda, Marta; Gimeno Cardona, Concepción

    2014-02-01

    The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) include controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology and molecular microbiology. This article presents the most relevant conclusions and lessons from the 2012 controls. As a whole, the results obtained in 2012 confirm the excellent skill and good technical standards found in previous editions. However, erroneous results can be obtained in any laboratory and in clinically relevant determinations. Once again, the results of this program highlighted the need to implement both internal and external controls in order to assure the maximal quality of the microbiological tests. Copyright © 2014 Elsevier España, S.L. All rights reserved.

  12. [Analysis of the results of the SEIMC External Quality Control Program. Year 2014].

    Science.gov (United States)

    Gopegui Bordes, Enrique Ruiz de; Guna Serrano, M Del Remedio; Orta Mira, Nieves; Medina González, Rafael; Rosario Ovies, María; Poveda, Marta; Gimeno Cardona, Concepción

    2016-07-01

    The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) include controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology and molecular microbiology. This article presents the most relevant conclusions and lessons from the 2014 controls. As a whole, the results obtained in 2014 confirm the excellent skill and good technical standards found in previous editions. However, erroneous results can be obtained in any laboratory and in clinically relevant determinations. Once again, the results of the SEIMC program highlighted the need to implement both internal and external controls in order to assure the maximal quality of the microbiological tests. Copyright © 2016 Elsevier España, S.L.U. All rights reserved.

  13. [Analysis of the results of the SEIMC External Quality Control Program. Year 2008].

    Science.gov (United States)

    Serrano, María del Remedio Guna; Mira, Nieves Orta; de Gopegui, Enrique Ruiz; Ovies, María Rosario; Cardona, Concepción Gimeno; Pérez, José L

    2010-01-01

    The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) include controls for bacteriology, serology, mycology, parasitology, mycobacteria and virology. This article present the most relevant conclusions and lessons from the 2008 controls. As a whole, the results obtained in 2008 confirm the excellent skill and good technical standards of the microbiology laboratories in Spain found in previous editions. However, a few deviations can be obtained in any laboratory, even in clinically relevant determinations. Once again, the results of this program highlighted the need to implement both internal an external controls in order to assure the maximal quality of the microbiological tests. 2010 Elsevier España S.L. All rights reserved.

  14. [Analysis of the results of the SEIMC External Quality Control Program. Year 2009].

    Science.gov (United States)

    de Gopegui Bordes, Enrique Ruiz; del Remedio Guna Serrano, M; Orta Mira, Nieves; Ovies, María; Poveda, Marta; Gimeno Cardona, Concepción; Pérez, José L

    2011-03-01

    The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) includes controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology and molecular microbiology. In this article, the most important conclusions and lessons from the 2009 controls are presented. As a whole, the results obtained in 2009 confirm the excellent skill and good technical standards found in previous editions. However, erroneous results can be obtained in any laboratory and in clinically relevant determinations. The results of this program highlight the need to implement both internal and external controls in order to ensure maximal quality of microbiological tests. Copyright © 2011 Elsevier España S.L. All rights reserved.

  15. An improved radiofrequency method to pasteurize salmonella in shell eggs

    Science.gov (United States)

    The goals of this study were to improve radio frequency (RF) shell egg pasteurization technology to make it more commercially feasible, to evaluate its efficacy in reducing Salmonella, and to determine its effect on the quality changes of the egg. The original RF technology inactivated 6.8 log of E....

  16. External quality assessments for microbiologic diagnosis of diphtheria in Europe.

    Science.gov (United States)

    Both, Leonard; Neal, Shona; De Zoysa, Aruni; Mann, Ginder; Czumbel, Ida; Efstratiou, Androulla

    2014-12-01

    The European Diphtheria Surveillance Network (EDSN) ensures the reliable epidemiological and microbiologic assessment of disease prevalence in the European Union. Here, we describe a survey of current diagnostic techniques for diphtheria surveillance conducted across the European Union and report the results from three external quality assessment (EQA) schemes performed between 2010 and 2014. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  17. Fertilization Induces a Transient Exposure of Phosphatidylserine in Mouse Eggs

    Science.gov (United States)

    Curia, Claudio A.; Ernesto, Juan I.; Stein, Paula; Busso, Dolores; Schultz, Richard M.; Cuasnicu, Patricia S.; Cohen, Débora J.

    2013-01-01

    Phosphatidylserine (PS) is normally localized to the inner leaflet of the plasma membrane and the requirement of PS translocation to the outer leaflet in cellular processes other than apoptosis has been demonstrated recently. In this work we investigated the occurrence of PS mobilization in mouse eggs, which express flippase Atp8a1 and scramblases Plscr1 and 3, as determined by RT-PCR; these enzyme are responsible for PS distribution in cell membranes. We find a dramatic increase in binding of flouresceinated-Annexin-V, which specifically binds to PS, following fertilization or parthenogenetic activation induced by SrCl2 treatment. This increase was not observed when eggs were first treated with BAPTA-AM, indicating that an increase in intracellular Ca2+ concentration was required for PS exposure. Fluorescence was observed over the entire egg surface with the exception of the regions overlying the meiotic spindle and sperm entry site. PS exposure was also observed in activated eggs obtained from CaMKIIγ null females, which are unable to exit metaphase II arrest despite displaying Ca2+ spikes. In contrast, PS exposure was not observed in TPEN-activated eggs, which exit metaphase II arrest in the absence of Ca2+ release. PS exposure was also observed when eggs were activated with ethanol but not with a Ca2+ ionophore, suggesting that the Ca2+ source and concentration are relevant for PS exposure. Last, treatment with cytochalasin D, which disrupts microfilaments, or jasplakinolide, which stabilizes microfilaments, prior to egg activation showed that PS externalization is an actin-dependent process. Thus, the Ca2+ rise during egg activation results in a transient exposure of PS in fertilized eggs that is not associated with apoptosis. PMID:23951277

  18. Intraclutch eggshell colour variation in birds: are females able to identify their eggs individually?

    Directory of Open Access Journals (Sweden)

    Miroslav Poláček

    2017-08-01

    Full Text Available Background One possibility suggested regarding female post-mating strategies is differential allocation into offspring investment. Female birds produce not only the largest, but also most colourful eggs of all oviparous taxa. Larger eggs provide space for bigger embryos, or more nutrition for their development, but the question why eggs are more colourful and why there is variation in eggshell colouration remains. In this context, the focus of interest has been to explain inter-clutch variation but in many bird species, eggshell colouration also varies within a clutch. Surprisingly, less attention has been paid to this phenomenon. Therefore, we propose the “female egg recognition” hypothesis, suggesting that mothers use colour characteristics to interpret egg attributes and allocate further investment into each egg accordingly. To evaluate the feasibility of the hypothesis, we tested several underlying predictions and examined their suitability using a dataset from our tree sparrow (Passer montanus study. We predict (i substantial within-clutch variation in eggshell colouration which, (ii should be related to laying sequence, (iii reflect egg quality and, (iv should stimulate a female response. Methods Eggshell coloration data were obtained via digital photography under standardized conditions, taken after clutch completion. Lightness (L*, representing the achromatic properties of an egg has been chosen as the most important predictor in dark cavities and was related to egg quality and position in the nest. Results In our tree sparrows, first and mainly last eggs were less pigmented, providing information about laying order. Egg volume, which predicts chick quality, positively correlates with eggshell coloration. Finally, we could show that female tree sparrows placed darker, but not bigger, eggs into more central incubation positions. Discussion All basic prerequisites for the “female egg recognition” hypothesis are fulfilled. In this

  19. Internal quality control indicators of cervical cytopathology exams performed in laboratories monitored by the External Quality Control Laboratory.

    Science.gov (United States)

    Ázara, Cinara Zago Silveira; Manrique, Edna Joana Cláudio; Tavares, Suelene Brito do Nascimento; de Souza, Nadja Lindany Alves; Amaral, Rita Goreti

    2014-09-01

    To evaluate the impact of continued education provided by an external quality control laboratory on the indicators of internal quality control of cytopathology exams. The internal quality assurance indicators for cytopathology exams from 12 laboratories monitored by the External Quality Control Laboratory were evaluated. Overall, 185,194 exams were included, 98,133 of which referred to the period preceding implementation of a continued education program, while 87,061 referred to the period following this intervention. Data were obtained from the Cervical Cancer Database of the Brazilian National Health Service. Following implementation of the continued education program, the positivity index (PI) remained within recommended limits in four laboratories. In another four laboratories, the PI progressed from below the limits to within the recommended standards. In one laboratory, the PI remained low, in two laboratories, it remained very low, and in one, it increased from very low to low. The percentage of exams compatible with a high-grade squamous intraepithelial lesion (HSIL) remained within the recommended limits in five laboratories, while in three laboratories it progressed from below the recommended levels to >0.4% of the total number of satisfactory exams, and in four laboratories it remained below the standard limit. Both the percentage of atypical squamous cells of undetermined significance (ASC-US) in relation to abnormal exams, and the ratio between ASC-US and intraepithelial lesions remained within recommended levels in all the laboratories investigated. An improvement was found in the indicators represented by the positivity index and the percentage of exams compatible with a high-grade squamous intraepithelial lesion, showing that the role played by the external quality control laboratory in providing continued education contributed towards improving laboratory staff skills in detecting cervical cancer precursor lesions.

  20. Oral food desensitization in children with IgE-mediated hen's egg allergy: a new protocol with raw hen's egg.

    Science.gov (United States)

    Meglio, Paolo; Giampietro, Paolo G; Carello, Rossella; Gabriele, Ida; Avitabile, Simona; Galli, Elena

    2013-02-01

    Hen's egg allergy affects young children and can cause severe allergic reactions. Avoidance results in dietary limitations and can affect the quality of life, especially in cases where potentially life-threatening reactions exist. Our objective was to desensitize children with moderate-severe IgE-mediated hen's egg allergy over a 6-month period, by introducing increasing and very gradual daily doses of raw hen's egg in order to enable the children to assume 25ml of this food, or to induce tolerance to the highest possible dose. The protocol foresaw the egg reintroduction in the home setting. In this randomized, controlled open study, 20 hen's egg allergic children (10 in the active group) were admitted. A convincing history or a positive double-blind placebo-controlled food challenge confirmed the diagnosis. Oral desensitization was performed with increasing doses starting from 0.27 mg of hen's egg proteins (1 drop of raw hen's egg diluted 1:100). We adopted an original, mathematically calculated protocol in order to ensure a constant, daily increment of doses. 8/10 children (80%) in the active group achieved the daily intake of 25ml over a 6-month period. One child (10%) could tolerate up to 2ml/day while another child (10%) failed the desensitization. Six months after enrolment only 2 children in the control group (20%) could tolerate hen's egg. We successfully desensitized 8/10 children with IgE-mediated hen's egg allergy in a 6-month period. The partial outcome in the child who could tolerate 2ml/day reduced the risk of severe reactions after unnoticed introduction of egg. A regular protocol that ensures a daily constant increase of doses helps to reduce possible adverse events, thus improving safety and effectiveness. © 2012 John Wiley & Sons A/S.

  1. Survival of Ascaris eggs and hygienic quality of human excreta in Vietnamese composting latrines

    DEFF Research Database (Denmark)

    Jensen, Peter K. M.; Phuc, Pham D.; Konradsen, Flemming

    2009-01-01

    this is the length of time that farmers have available to produce fertilizer between two cropping seasons. This study aimed to investigate whether hygienically safe fertilizer could be produced in the latrines within this period of time. Methods: By inoculating eggs of the helminth parasite indicator Ascaris suum...... from 9.4 to 11.6, a >99% die-off of eggs was obtained after 105 to 117 days of storage for all lime concentrations and 97% of eggs were nonviable after 88 days of storage. The most critical parameter found to determine the die-off process was the amount of ammonia (urine) in the excreta which indicates...... that longer storage periods are needed for parasite egg die-off if urine is separated from the excreta. Conclusion: By inactivating >99% of all A. suum eggs in human excreta during a storage period of only three months the commonly used Double Vault Composting (DVC) latrine, in which urine is not separated...

  2. Effects of No. 2 fuel oil on hatchability of marine and estuarine bird eggs

    Science.gov (United States)

    White, Donald H.; King, Kirke A.; Coon, Nancy C.

    1979-01-01

    Oil spills and discharges may kill birds directly by destroying the insulation that their feathers provide so that they die of exposure; by poisoning them if they ingest oil; and by stressing them so that they starve to death. But oil pollution has more subtle effects, too. Nesting birds exposed to sublethal quantities of oil may transfer the oil to eggs in their nests, thereby causing failure of the eggs to hatch (RITTINGHAUS 1956). Laboratory studies have shown that very small quantities of oil, when applied to eggs of waterfowl, significantly reduced hatchability (HARTUNG 1965, ALBERS 1977a, SZARO and ALBERS 1977). The objective of this study was to determine the effects of external applications of No. 2 fuel oil on embryo survival of naturally and of artificially incubated eggs of marine and estuarine birds.

  3. Analytical performance specifications for external quality assessment - definitions and descriptions.

    Science.gov (United States)

    Jones, Graham R D; Albarede, Stephanie; Kesseler, Dagmar; MacKenzie, Finlay; Mammen, Joy; Pedersen, Morten; Stavelin, Anne; Thelen, Marc; Thomas, Annette; Twomey, Patrick J; Ventura, Emma; Panteghini, Mauro

    2017-06-27

    External Quality Assurance (EQA) is vital to ensure acceptable analytical quality in medical laboratories. A key component of an EQA scheme is an analytical performance specification (APS) for each measurand that a laboratory can use to assess the extent of deviation of the obtained results from the target value. A consensus conference held in Milan in 2014 has proposed three models to set APS and these can be applied to setting APS for EQA. A goal arising from this conference is the harmonisation of EQA APS between different schemes to deliver consistent quality messages to laboratories irrespective of location and the choice of EQA provider. At this time there are wide differences in the APS used in different EQA schemes for the same measurands. Contributing factors to this variation are that the APS in different schemes are established using different criteria, applied to different types of data (e.g. single data points, multiple data points), used for different goals (e.g. improvement of analytical quality; licensing), and with the aim of eliciting different responses from participants. This paper provides recommendations from the European Federation of Laboratory Medicine (EFLM) Task and Finish Group on Performance Specifications for External Quality Assurance Schemes (TFG-APSEQA) and on clear terminology for EQA APS. The recommended terminology covers six elements required to understand APS: 1) a statement on the EQA material matrix and its commutability; 2) the method used to assign the target value; 3) the data set to which APS are applied; 4) the applicable analytical property being assessed (i.e. total error, bias, imprecision, uncertainty); 5) the rationale for the selection of the APS; and 6) the type of the Milan model(s) used to set the APS. The terminology is required for EQA participants and other interested parties to understand the meaning of meeting or not meeting APS.

  4. Computation of External Quality Factors for RF Structures by Means of Model Order Reduction and a Perturbation Approach

    CERN Document Server

    Flisgen, Thomas; van Rienen, Ursula

    2016-01-01

    External quality factors are significant quantities to describe losses via waveguide ports in radio frequency resonators. The current contribution presents a novel approach to determine external quality factors by means of a two-step procedure: First, a state-space model for the lossless radio frequency structure is generated and its model order is reduced. Subsequently, a perturbation method is applied on the reduced model so that external losses are accounted for. The advantage of this approach results from the fact that the challenges in dealing with lossy systems are shifted to the reduced order model. This significantly saves computational costs. The present paper provides a short overview on existing methods to compute external quality factors. Then, the novel approach is introduced and validated in terms of accuracy and computational time by means of commercial software.

  5. EXTERNAL QUALITY ASSESSMENT OF GENERAL EDUCATION AS A FACTOR OF TEACHERS’ PROFESSIONAL DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    T. V. Potemkinа

    2017-01-01

    Full Text Available Introduction. Nowadays, foreign and Russian researchers state the lack of reliable tools for diagnostics of professional gaps among teachers. It is unjustified to draw conclusions about skill level of teachers, relying only on results which are shown by pupils when holding unified state examinations and monitoring tests. However, information on results of an external assessment of quality of the general education (the USE, OGE, All-Russian test works and national researches of quality of education corresponds to professional development of teachers and more rational organization of this process.The aim of the article is to consider and discuss the relationship between professional development of teachers and external assessment of the quality of general education; the search of opportunities and terms of the results use of external evaluation procedures in teacher training.Methodology and research methods. The authors analyzed the publications (available on the Internet and in periodicals to determine the relevance of the problem and compare approaches to its solution by Russian and foreign researchers. The methods involve content analysis, problem-based and comparative analysis for the diagnosis of the current practices of teachers’ professional development. The purpose of the analysis is the inclusion of education quality assessment and evaluation results in the contents of programs of professional development of teachers.Results. The created request for the profitability analysis results of an external assessment of general education quality of professional development of pedagogical staff is observed. However, the analysis of modern practice of additional professional pedagogical education showed that the preparation to prevails; certification procedure results for improvement of pedagogical activity are rarely used. The main reasons for this situation are found out: lack of the target information products for system of professional

  6. Maize kernel size and texture: production parameters, quality of eggs of the laying hens and electricity intake

    Directory of Open Access Journals (Sweden)

    Javer Alves Vieira Filho

    2015-08-01

    Full Text Available The influence of maize corn size and texture on the performance parameters of laying hens and power consumption required for grinding maize corn were evaluated. The experiment was carried out on 384 Isa Brown hens, 36 weeks old, penned in a conventional aviary with 562.5 cm2 bird-1 stocking rate. The treatments were distributed in a completely randomized 2 x 3 factorial design (maize textures: flint and dent; and milling degree: fine, medium and coarse with eight replicates of eight birds per plot. Data were evaluated with SISVAR and means were compared by Tukey’s test at 5% probability. Difference was reported for the variable texture and flint increased the variables feed intake and egg weight. Significant difference in the characteristics of egg quality occurred only for the colorof the yolk. Larger corn sizes consumed less electricity during grinding. The maize flint cultivar had a lower 31.7% power consumption when compared to that of the dent cultivar.

  7. The Effect of Calcium Source in Laying Hen Diet on Egg and Tibia Bone Characteristics

    International Nuclear Information System (INIS)

    Elsayed, M.A.; Basuony, H.A.; Hatab, M.H.

    2014-01-01

    One hundred and twenty, 37-week-old Lohmann strain layers were used in the current study to evaluate the effect of using ground, sterilized chicken eggshell (ES) as calcium (Ca) source in their diets on the productive performance, egg and eggshell quality, serum Ca and inorganic P concentrations and bone mineralization. Feed and water ad libitum were provided and hens were kept under 16:8 hrs light:dark cycle. Birds were randomly divided into 3 groups (GP), 1, was a control group fed a layer diet containing finely ground limestone as the Ca source. 2 was fed a layer diet that contained a combination of 50% limestone and 50% ground eggshell (50% ES) as the Ca source, whereas group 3 received a layer diet containing 100% ground eggshell (100% ES) as the Ca source in the diet. After 2 wk of acclimation, the birds were fed the experimental diets for 6 wk, initial and final body weights (BW) and feed intake was recorded. Eggs production was expressed as a percentage of hen-day egg production, egg weight and the internal egg quality were measured. Serum Ca, P concentrations, Ca:P ratio and aldosterone level were determined. Finally, tibia weight, length and its contents of Ca, P concentrations were measured. Results of this study indicated that, there was no significant effect of dietary treatment on BW and feed consumption. Replacing limestone in the current study diet with ground, sterilized eggshell had no any significant effect on egg production, egg weight, eggshell quality and tibia characteristics among between groups. Finally, there were no significant effect for treatment on blood hematocrit, total serum Ca, P concentrations, Ca:P ratio and aldosterone level. It is concluded that hatchery waste, as chicken egg shells, can be used as the Ca source in layer diets without an adverse effect on BW, feed consumption, egg weight, egg production, egg and eggshell quality, serum Ca concentration and bone characteristics

  8. Parenting and Friendship Quality as Predictors of Internalizing and Externalizing Symptoms in Early Adolescence

    Science.gov (United States)

    Gaertner, Alden E.; Fite, Paula J.; Colder, Craig R.

    2010-01-01

    Research indicates both parents and peers influence child and adolescent adjustment outcomes. Moreover, friendship quality has been found to buffer the influence of parenting on adolescent adjustment, particularly externalizing symptoms. Little to no research, however, has longitudinally examined whether friendship quality moderates the relation…

  9. Egg temperature and embryonic metabolism of A- and B-eggs of ...

    African Journals Online (AJOL)

    Macaroni and rockhopper penguins lay two eggs but rear only one chick to independence. The eggs are markedly dimorphic in size and, although the smaller A-egg is laid several days before the B-egg, in nests where both eggs are incubated, the B-egg always hatches first. Incubation temperatures and embryonic oxygen ...

  10. Decreased sexual capacity after external radiation therapy for prostate cancer impairs quality of life

    International Nuclear Information System (INIS)

    Helgason, Asgeir R.; Fredrikson, Mats; Adolfsson, Jan; Steineck, Gunnar

    1995-01-01

    Purpose: The aim of this study was to assess to what extent patients treated with radiation therapy for prostate cancer experience change in sexual functioning and to what extent this effects quality of life. Methods and Materials: Information was provided by 53 men treated with radiation therapy for localized prostate cancer. Assessment was made with the ''Radiumhemmets Scale of Sexual Functioning,'' which measures sexual desire, erectile capacity, orgasm, and to what extent a decrease in any of these aspects of sexual functioning affects quality of life. Function before treatment was assessed retrospectively. Results: Sexual desire diminished among 77% after treatment. The erection stiffness decreased in 77%. Before external radiation therapy, 66% had an erection usually sufficient for intercourse. Half of the men lost this ability after treatment. Of those retaining orgasm after treatment, 47% reported a decreased orgasmic pleasure and 91% a reduced ejaculation volume. Of all men, 50% reported that quality of life had decreased much or very much due to a decline in the erectile capacity following external radiation therapy. Conclusion: The results of the present study indicate that external radiation therapy for prostate cancer is associated with a reduction in sexual desire, erectile capacity, and orgasm functions. In a majority of patients this reduces quality of life. Previously, we may have underestimated the importance an intact sexual function has for the quality of life in this patient category of elderly men

  11. Prediction of egg freshness during storage using electronic nose.

    Science.gov (United States)

    Yimenu, Samuel M; Kim, J Y; Kim, B S

    2017-10-01

    The aim of the present study was to investigate the potential of a fast gas chromatography (GC) e-nose for freshness discrimination and for prediction of storage time as well as sensory and internal quality changes during storage of hen eggs. All samples were obtained from the same egg production farm and stored at 20 °C for 20 d. Egg sampling was conducted every 0, 3, 6, 9, 12, 16, and 20 d. During each sampling time, 4 egg cartons (each containing 10 eggs) were randomly selected: one carton for Haugh units, one carton for sensory evaluation and 2 cartons for the e-nose experiment. The e-nose study included 2 independent test sets; calibration (35 samples) and validation (28 samples). Every sampling time, 5 replicates were prepared from one egg carton for calibration samples and 4 replicates were prepared from the remaining egg carton for validation samples. Sensors (peaks) were selected prior to multivariate chemometric analysis; qualitative sensors for principal component analysis (PCA) and discriminant factor analysis (DFA) and quantitative sensors for partial least square (PLS) modeling. PCA and DFA confirmed the difference in volatile profiles of egg samples from 7 different storage times accounting for a total variance of 95.7% and 93.71%, respectively. Models for predicting storage time, Haugh units, odor score, and overall acceptability score from e-nose data were developed using calibration samples by PLS regression. The results showed that these quality indices were well predicted from the e- nose signals, with correlation coefficients of R2 = 0.9441, R2 = 0.9511, R2 = 0.9725, and R2 = 0.9530 and with training errors of 0.887, 1.24, 0.626, and 0.629, respectively. As a result of ANOVA, most of the PLS model results were not significantly (P > 0.05) different from the corresponding reference values. These results proved that the fast GC electronic nose has the potential to assess egg freshness and feasibility to predict multiple egg freshness indices

  12. MCNP simulation of the influence of the external moisture on low calorific value in the coal quality analysis by neutron

    International Nuclear Information System (INIS)

    Liu Dekun; Zhang Hongyu; Zhang Lihong; Dong Huan; Gu Deshan

    2012-01-01

    An important index in assessment of coal quality is low calorific value. Using neutron to analysis coal quality, the more the coal moisture content, especially the increasing of external moisture will reduce the low calorific value. The principle of coal quality analysis by neutron prompt Gamma-ray is introduced. The influence of the gamma count of the carbon element peak with increasing external moisture in coal samples was simulated using MCNP code. And discussed the reasons how external moisture content influence the calorific value. Simulation results indicate that with the increasing of external moisture in the coal samples, the gamma count of the carbon element peak dwindling, and the low calorific value reducing. The conclusion is : using neutrons method to analysis coal quality, the more external moisture content, the larger error of the measurement results of the carbon element, and will influence the calculation accuracy of the low calorific value. (authors)

  13. Hatchery waste: nutritional evaluation of non-hatched eggs.

    Science.gov (United States)

    de Souza, M L; Jokl, L; da Silva, J M; Vieira, E C

    1978-12-01

    Hen's non-hatched eggs were processed by boiling for 30 minutes, milling in a meat grinder, and drying at 60 degrees C with continuous ventilation. The product contained 36% of protein, 27% of ether extract, 17% of ash, 10% of calcium, and 0.6% of phosphorus. The quality of the protein was comparable to that of a reference casein and of fresh egg meal, as determined by protein efficiency ratio and apparent net protein utilization.

  14. Relationships between egg-recognition and egg-ejection in a grasp-ejector species.

    Directory of Open Access Journals (Sweden)

    Manuel Soler

    Full Text Available Brood parasitism frequently leads to a total loss of host fitness, which selects for the evolution of defensive traits in host species. Experimental studies have demonstrated that recognition and rejection of the parasite egg is the most common and efficient defence used by host species. Egg-recognition experiments have advanced our knowledge of the evolutionary and coevolutionary implications of egg recognition and rejection. However, our understanding of the proximate mechanisms underlying both processes remains poor. Egg rejection is a complex behavioural process consisting of three stages: egg recognition, the decision whether or not to reject the putative parasitic egg and the act of ejection itself. We have used the blackbird (Turdus merula as a model species to explore the relationship between egg recognition and the act of egg ejection. We have manipulated the two main characteristics of parasitic eggs affecting egg ejection in this grasp-ejector species: the degree of colour mimicry (mimetic and non-mimetic, which mainly affects the egg-recognition stage of the egg-rejection process and egg size (small, medium and large, which affects the decision to eject, while maintaining a control group of non-parasitized nests. The behaviour of the female when confronted with an experimental egg was filmed using a video camera. Our results show that egg touching is an indication of egg recognition and demonstrate that blackbirds recognized (i.e., touched non-mimetic experimental eggs significantly more than mimetic eggs. However, twenty per cent of the experimental eggs were touched but not subsequently ejected, which confirms that egg recognition does not necessarily mean egg ejection and that accepting parasitic eggs, at least sometimes, is the consequence of acceptance decisions. Regarding proximate mechanisms, our results show that the delay in egg ejection is not only due to recognition problems as usually suggested, given that experimental

  15. Quality assurance of external exposure measurement for national survey of environmental natural radioactive level

    International Nuclear Information System (INIS)

    Yue Qingyu

    1995-01-01

    This paper summarizes the quality assurance work of external exposure measurement for national survey of environmental natural radioactive level. It mainly introduces instrumentation used in external exposure measurement and its properties, the measurement results of three times of national in-site intercomparison, and in-site sample check results of measurement results from 29 provinces, cities and autonomous regions and Wuhan, Baotou cities

  16. External quality assessment programs in the context of ISO 15189 accreditation.

    Science.gov (United States)

    Sciacovelli, Laura; Secchiero, Sandra; Padoan, Andrea; Plebani, Mario

    2018-05-23

    Effective management of clinical laboratories participating in external quality assessment schemes (EQAS) is of fundamental importance in ensuring reliable analytical results. The International Standard ISO 15189:2012 requires participation in interlaboratory comparison [e.g. external quality assessment (EQA)] for all tests provided by an individual laboratory. If EQAS is not commercially available, alternative approaches should be identified, although clinical laboratories may find it challenging to choose the EQAS that comply with the international standards and approved guidelines. Great competence is therefore required, as well as knowledge of the characteristics and key elements affecting the reliability of an EQAS, and the analytical quality specifications stated in approved documents. Another skill of fundamental importance is the ability to identify an alternative approach when the available EQAS are inadequate or missing. Yet the choice of the right EQA program alone does not guarantee its effectiveness. In fact, the fundamental steps of analysis of the information provided in EQA reports and the ability to identify improvement actions to be undertaken call for the involvement of all laboratory staff playing a role in the specific activity. The aim of this paper was to describe the critical aspects that EQA providers and laboratory professionals should control in order to guarantee effective EQAS management and compliance with ISO 15189 accreditation requirements.

  17. Scientific and technological aspects of the radiopasteurization of egg products

    Energy Technology Data Exchange (ETDEWEB)

    Etienne, J C; Biltiau, J; Rombaux, J P

    1973-11-01

    A bibliographic review is presented of the scientific and technological problems in the thermal pasteurization of egg products and the possible advantages of the substitution of radiopasteurization. Current procedures of pasteurization are described. Conditioning of the egg products by congealing or dehydration prior to storage is discussed. A comparative examination is made of the physico-chemical properties of treated egg products and of the organoleptic qualities of the finished product. The present status of radiopasteurization, the problems to be solved, and the economic aspects are discussed. (JSR)

  18. Life-history consequences of investment in free-spawned eggs and their accessory coats.

    Science.gov (United States)

    Podolsky, Robert D

    2004-05-01

    The optimal trade-off between offspring size and number can depend on details of the mode of reproduction or development. In marine organisms, broadcast spawning is widespread, and external coats are a common feature of spawned eggs. Egg jelly coats are thought to influence several aspects of fertilization and early development, including the size of the target for sperm, fertilization efficiency, egg suspension time, polyspermy, embryo survival, and fecundity. These costs and benefits of investment in jelly result in trade-offs that can influence optimal reproductive allocation and the evolution of egg size. I develop an optimization model that sequentially incorporates assumptions about the function of egg coats in fertilization. The model predicts large variation in coat size and limited variation in ovum size under a broad range of conditions. Heterogeneity among spawning events further limits the range of ovum sizes predicted to evolve under sperm limitation. In contrast, variation in larval mortality predicts a broad range of optimal ovum sizes that more closely reflects natural variation among broadcast-spawning invertebrates. By decoupling physical and energetic size, egg coats can enhance fertilization, maintain high fecundity, and buffer the evolution of ovum size from variation in spawning conditions.

  19. Egg laying sequence influences egg mercury concentrations and egg size in three bird species: Implications for contaminant monitoring programs

    Science.gov (United States)

    Ackerman, Joshua T.; Eagles-Smith, Collin A.; Herzog, Mark P.; Yee, Julie L.; Hartman, C. Alex

    2016-01-01

    Bird eggs are commonly used in contaminant monitoring programs and toxicological risk assessments, but intra-clutch variation and sampling methodology could influence interpretability. We examined the influence of egg laying sequence on egg mercury concentrations and burdens in American avocets, black-necked stilts, and Forster's terns. The average decline in mercury concentrations between the first and last egg laid was 33% for stilts, 22% for terns, and 11% for avocets, and most of this decline occurred between the first and second eggs laid (24% for stilts, 18% for terns, and 9% for avocets). Trends in egg size with egg laying order were inconsistent among species and overall differences in egg volume, mass, length, and width were mercury concentrations generally declined by 16% between the first and second eggs laid. Despite the strong effect of egg laying sequence, most of the variance in egg mercury concentrations still occurred among clutches (75%-91%) rather than within clutches (9%-25%). Using simulations, we determined that to accurately estimate a population's mean egg mercury concentration using only a single random egg from a subset of nests, it would require sampling >60 nests to represent a large population (10% accuracy) or ≥14 nests to represent a small colony that contained <100 nests (20% accuracy).

  20. Recombinant egg drop syndrome subunit vaccine offers an alternative to virus propagation in duck eggs.

    Science.gov (United States)

    Gutter, B; Fingerut, E; Gallili, G; Eliahu, D; Perelman, B; Finger, A; Pitcovski, J

    2008-02-01

    Egg drop syndrome (EDS) virus vaccines are routinely produced in embryonated duck eggs (Solyom et al., 1982). This procedure poses the risk of dissemination of pathogens, such as avian influenza virus, as the eggs used are not from specific pathogen free birds. To address this problem, the knob and part of the shaft domain of the fibre protein of the EDS virus (termed knob-s) were expressed in Escherichia coli and assessed as a subunit vaccine. A single vaccination with the recombinant protein induced the production of anti-EDS virus antibodies, as detected by haemagglutination inhibition, enzyme-linked immunosorbent assay and virus neutralization tests, for at least 20 weeks. A positive correlation was demonstrated between these three assays. A dose-response assessment showed that the vaccine was effective over the range of 2 to 64 microg protein per dose. Two vaccinations with the recombinant protein, administered before the onset of lay, induced high haemagglutination inhibition antibody titres, comparable with those induced by an inactivated whole-virus vaccine. The vaccine did not have any adverse effects on egg production, quality or weight. The present study has shown that two vaccinations with the recombinant knob-s protein elicited high neutralizing antibody titres that persisted for more than 50 weeks of lay.

  1. Guidelines for the preparation of a quality manual for external audit groups on dosimetry in radiotherapy

    International Nuclear Information System (INIS)

    Izewska, Joanna; Arib, M.; Saravi, M.

    2002-01-01

    This document has been prepared within the framework of a Co-ordinated Research Programme (CRP) on Development of Quality Assurance Programme for Radiation Therapy Dosimetry in Developing Countries, during two Meetings at the IAEA Headquarters in Vienna (11-14 November 1996 and 6-10 October 1997). It is based on the recommendations of ISO 9000 series and ISO/IEC guide No. 25. The document can be used as a guide on how to prepare a quality manual for national External Audit Groups (EAG), i.e., a nationally recognised group in charge of operating external quality audits for radiotherapy dosimetry. The EAG of a given country includes the SSDL, a Measuring Group and a Medical Physics Group, who work in close co-operation at all steps of the audit. The content herein should be considered as a suggestion and additions or deletions can be made in accordance with the specific conditions in each country. It is preferable that the manual itself be as concise as possible, limiting it to the core scope. Detailed working sheets describing the procedures should be included in Appendices together with data sheets, questionnaires and reporting forms. The quality manual of each country should be carefully reviewed by all members of the EAG and, as far as possible, should be approved by relevant professional bodies and supported by health authorities. It has long been recognised that accurate knowledge of the dose in radiotherapy is vital to ensure safe and effective radiation treatments. To achieve this goal, comprehensive quality assurance programmes should be established to cover all steps from dose prescription to dose delivery. These programmes should include internal checks performed by the radiotherapy centres and external audits made by independent external bodies. It is estimated that not more than 50% of radiotherapy facilities world-wide have participated in some level of dose quality audit by an independent expert. Genuine concern exists that some, or even many

  2. The role of egg-nest contrast in the rejection of brood parasitic eggs.

    Science.gov (United States)

    Aidala, Zachary; Croston, Rebecca; Schwartz, Jessica; Tong, Lainga; Hauber, Mark E

    2015-04-15

    Hosts of avian brood parasites can avoid the reproductive costs of raising genetically unrelated offspring by rejecting parasitic eggs. The perceptual cues and controls mediating parasitic egg discrimination and ejection are well studied: hosts are thought to use differences in egg color, brightness, maculation, size and shape to discriminate between their own and foreign eggs. Most theories of brood parasitism implicitly assume that the primary criteria to which hosts attend when discriminating eggs are differences between the eggs themselves. However, this assumption is confounded by the degree to which chromatic and achromatic characteristics of the nest lining co-vary with egg coloration, so that egg-nest contrast per se might be the recognition cue driving parasitic egg detection. Here, we systematically tested whether and how egg-nest contrast itself contributes to foreign egg discrimination. In an artificial parasitism experiment, we independently manipulated egg color and nest lining color of the egg-ejector American robin (Turdus migratorius), a host of the obligate brood parasitic brown-headed cowbird (Molothrus ater). We hypothesized that the degree of contrast between foreign eggs and the nest background would affect host egg rejection behavior. We predicted that experimentally decreasing egg-nest chromatic and achromatic contrast (i.e. rendering parasitic eggs more cryptic against the nest lining) would decrease rejection rates, while increasing egg-nest contrast would increase rejection rates. In contrast to our predictions, egg-nest contrast was not a significant predictor of egg ejection patterns. Instead, egg color significantly predicted responses to parasitism. We conclude that egg-egg differences are the primary drivers of egg rejection in this system. Future studies should test for the effects of egg-nest contrast per se in predicting parasitic egg recognition in other host-parasite systems, including those hosts building enclosed nests and

  3. Ostrich eggs geometry

    Directory of Open Access Journals (Sweden)

    Šárka Nedomová

    2013-01-01

    Full Text Available Precise quantification of the profile of egg can provide a powerful tool for the analysis of egg shape for various biological problems. A new approach to the geometry of a Ostrich’s egg profile is presented here using an analysing the egg’s digital photo by edge detection techniques. The obtained points on the eggshell counter are fitted by the Fourier series. The obtained equations describing an egg profile have been used to calculate radii of curvature. The radii of the curvature at the important point of the egg profile (sharp end, blunt end and maximum thickness are independent on the egg shape index. The exact values of the egg surface and the egg volume have been obtained. These quantities are also independent on the egg shape index. These quantities can be successively estimated on the basis of simplified equations which are expressed in terms of the egg length, L¸ and its width, B. The surface area of the eggshells also exhibits good correlation with the egg long circumference length. Some limitations of the most used procedures have been also shown.

  4. Pasture, multi-enzymes, benzoic acid and essential oils positively influence performance, intestinal organ weight and egg quality in free-range laying hens.

    Science.gov (United States)

    Iqbal, Z; Roberts, J; Perez-Maldonado, R A; Goodarzi Boroojeni, F; Swick, R A; Ruhnke, I

    2018-04-01

    1. The objective of this study was to investigate the effect of range type, multi-enzyme applications, and a combination of benzoic acid (BA) and essential oils (EO) on the productive performance, organ weight and egg quality of free-range laying hens. 2. Three hundred laying hens were evaluated for the short-term (6 weeks) and long-term (12 weeks) effects of range type (G = no pasture, P = pasture) and feed additives (T1 = control; T2 = betaglucanase/pectinase/protease; T3 = BA/EO). Body weight, feed intake (FI), feed conversion ratio (FCR), egg production (EP), digestive organ weight, and egg quality (EQ) were evaluated. Data were analysed using SPSS 2.2 in a 2×2×3 factorial arrangement. 3. Hens that ranged on pasture were significantly heavier (2043 g vs. 1996 g; p ranged on gravel. Hens fed T2 were significantly heavier (2050 g) compared to hens fed T1 (2005 g) or T3 (2008 g). Organ weights (gizzard, liver and pancreas) were significantly heavier in hens ranged on pasture (16.8 g/kg BW, 22.3 g/kg BW and 1.89 g/kg BW, respectively) compared to hens ranged on gravel (14.2 g/kg BW, 21.7 g/kg BW and 1.83 g/kg BW, respectively). Over time, body weight (1970-2070 g; p < 0.001) and egg weight (59.5-62.8 g; p < 0.001) increased, FI (123-120 g; p = 0.024) was reduced and FCR (2.36-2.10; p = 0.002) improved 4. In conclusion, hens housed on pasture and fed multi-enzyme supplemented diets had significantly heavier body weight and produced heavier eggs with darker yolk colour. Pasture intake and enzyme supplementation increased digestive organ weight significantly.

  5. Suggested recommendations for a minimal programme of quality assurance in external radiotherapy

    International Nuclear Information System (INIS)

    Aletti, P.; Bey, P.; Brossens, M.; Chauvel, P.; Chavaudra, J.; Cohen-Solal, C.; Costa, A.; Desprez, P.; Donnarieix, D.; Gaboriaud, G.; Kinany, R.; Lagrange, J.L.; Manny, C.; Pontvert, D.; Rozan, R.; Valinta, D.; Van Dam, J.

    1991-01-01

    This article gives the suggested recommendations for a minimal programme of quality assurance in external radiotherapy made by a study group of the French Society of hospital physicists and the French Society of oncological radiation therapy with belgian physicists and radiotherapists [fr

  6. Laboratory diagnosis of the rare anaemias: external quality assessment benefits patient care

    Directory of Open Access Journals (Sweden)

    Barbara De La Salle

    2013-03-01

    Full Text Available Since its introduction in the 1960s, external quality assessment has developed to become an essential component of the quality management system of the diagnostic laboratory. External quality assessment provides a long term, retrospective view of laboratory performance, demonstrating the competence of the laboratory to others. The ENERCA project (the European Network for Rare and Congenital Anaemias has established a list of core laboratory tests that are used in the diagnosis of rare and congenital anaemias, which has been used as the basis for questionnaires to laboratories, to establish the use and quality assurance of diagnostic testing in the congenital and rare anaemias, and to European EQA providers for services in this key area. In general, the provision of EQA for rare and congenital anaemias is widely variable with little provision for the very rare disorders. For the more common congenital anaemias, such as the haemoglobinopathies and thalassaemias, provision is better but there is variation in aspects of the scheme design, especially the frequency of distribution. Where laboratories did not take part in EQA for individual tests, or there was no EQA available, a desire to participate was expressed in 66% (102/154 of cases. The provision of external quality assessment (EQA services for rare disorders is a challenge. For many of these conditions, the number of patients in any one member state is very small with only a few laboratories providing diagnostic testing. In these cases, the development of pan-European or cross-border EQA may be the only means by which standardisation of methods and results can be achieved. An EQA survey of 243 laboratories for performance in Hb A2 quantification showed encouraging results in that there was a clear differentiation in the results from a beta Thalassaemia carrier and an individual with no evidence of Thalassaemia; however, a bias was observed between different methods of measurement.

  7. External quality assurance for image grading in the Scottish Diabetic Retinopathy Screening Programme.

    Science.gov (United States)

    Goatman, K A; Philip, S; Fleming, A D; Harvey, R D; Swa, K K; Styles, C; Black, M; Sell, G; Lee, N; Sharp, P F; Olson, J A

    2012-06-01

    To develop and evaluate an image grading external quality assurance system for the Scottish Diabetic Retinopathy Screening Programme. A web-based image grading system was developed which closely matches the current Scottish national screening software. Two rounds of external quality assurance were run in autumn 2008 and spring 2010, each time using the same 100 images. Graders were compared with a consensus standard derived from the top-level graders' results. After the first round, the centre lead clinicians and top-level graders reviewed the results and drew up guidance notes for the second round. Grader sensitivities ranged from 60.0 to 100% (median 92.5%) in 2008, and from 62.5 to 100% (median 92.5%) in 2010. Specificities ranged from 34.0 to 98.0% (median 86%) in 2008, and 54.0 to 100% (median 88%) in 2010. There was no difference in sensitivity between grader levels, but first-level graders had a significantly lower specificity than level-two and level-three graders. In 2008, one centre had a lower sensitivity but higher specificity than the majority of centres. Following the feedback from the first round, overall agreement improved in 2010 and there were no longer any significant differences between centres. A useful educational tool has been developed for image grading external quality assurance. © 2011 The Authors. Diabetic Medicine © 2011 Diabetes UK.

  8. Effects of dietary n-3 and n-6 fatty acids levels on egg and larval quality of Eurasian perch

    DEFF Research Database (Denmark)

    Henrotte, E.; Overton, Julia Lynne; Kestemont, P.

    2008-01-01

    Three groups of 40 perch breeders were reared in order to study the effects of 3 different levels of dietary n-3 and n-6 fatty acids on egg quality. Two experimental diets, R1 and R2 (n-3/n-6 = 0.13 and 35.54, respectively), were compared to one commercial food, R3 (n-3/n-6 = 3.48.). Spawning and...

  9. Becoming a morther by non-anonymous egg donation: secrecy and the relationship between egg recipient, egg donor and egg donation child

    NARCIS (Netherlands)

    Berkel, D.A.M. van; Candido, A.; Pijffers, W.H.

    The object of the study was to investigate secrecy in non-anonymous egg donation, to explore some characteristics of this kind of egg donation arrangement and the relationship of the recipient with her non-genetic child. Forty-four egg recipients and 62 IVF patients with a child conceived through

  10. The evolution of egg colour and patterning in birds.

    Science.gov (United States)

    Kilner, R M

    2006-08-01

    Avian eggs differ so much in their colour and patterning from species to species that any attempt to account for this diversity might initially seem doomed to failure. Here I present a critical review of the literature which, when combined with the results of some comparative analyses, suggests that just a few selective agents can explain much of the variation in egg appearance. Ancestrally, bird eggs were probably white and immaculate. Ancient diversification in nest location, and hence in the clutch's vulnerability to attack by predators, can explain basic differences between bird families in egg appearance. The ancestral white egg has been retained by species whose nests are safe from attack by predators, while those that have moved to a more vulnerable nest site are now more likely to lay brown eggs, covered in speckles, just as Wallace hypothesized more than a century ago. Even blue eggs might be cryptic in a subset of nests built in vegetation. It is possible that some species have subsequently turned these ancient adaptations to new functions, for example to signal female quality, to protect eggs from damaging solar radiation, or to add structural strength to shells when calcium is in short supply. The threat of predation, together with the use of varying nest sites, appears to have increased the diversity of egg colouring seen among species within families, and among clutches within species. Brood parasites and their hosts have probably secondarily influenced the diversity of egg appearance. Each drives the evolution of the other's egg colour and patterning, as hosts attempt to avoid exploitation by rejecting odd-looking eggs from their nests, and parasites attempt to outwit their hosts by laying eggs that will escape detection. This co-evolutionary arms race has increased variation in egg appearance both within and between species, in parasites and in hosts, sometimes resulting in the evolution of egg colour polymorphisms. It has also reduced variation in

  11. Single and Combined Impacts of Vitamin A and Selenium in Diet on Productive Performance, Egg Quality, and Some Blood Parameters of Laying Hens During Hot Season.

    Science.gov (United States)

    Abd El-Hack, Mohamed E; Mahrose, Khalid; Askar, Ali A; Alagawany, Mahmoud; Arif, Muhammad; Saeed, Muhammad; Abbasi, Farzana; Soomro, Rab Nawaz; Siyal, Farman Ali; Chaudhry, Maria Tabassum

    2017-05-01

    A study was conducted using 162 Bovans laying hens to investigate the impacts of extra dietary vitamin A (0, 8000, 16,000 IU/kg), selenium (0, 0.25, 0.50 mg/kg), and their combinations on the performance, egg quality, and blood biological parameters of laying hens during summer months. Supplemental vitamin A up to 16,000 IU/kg diet significantly (P feed intake which increased with 8000 IU/kg diet compared with control. Feed intake and feed conversion of hens fed diet supplemented with selenium revealed high statistical (P = 0.001) differences. All egg quality criteria were not significantly (P hens reared under heat stress conditions.

  12. The effects of laying hen housing systems on egg safety and quality. In: Achieving sustainable production of eggs, Vol 1: Safety and Quality

    Science.gov (United States)

    Transitions in laying hen management and housing systems have constantly occurred throughout the history of commercial egg production. Around the world, there has been a rapid shift in hen housing requirements since the turn of the current century. In most cases, the changes in hen housing require...

  13. 9 CFR 590.410 - Shell eggs and egg products required to be labeled.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Shell eggs and egg products required..., DEPARTMENT OF AGRICULTURE EGG PRODUCTS INSPECTION INSPECTION OF EGGS AND EGG PRODUCTS (EGG PRODUCTS INSPECTION ACT) Identifying and Marking Product § 590.410 Shell eggs and egg products required to be labeled...

  14. The relationship of physical and chemical conditions of CEP diluent with egg yolk addition to bull spermatozoa quality before and after storage at temperaturof 4-5°C

    Science.gov (United States)

    Ducha, N.; Hariani, D.; Budijastuti, W.

    2018-01-01

    Storage of semen requires diluent to dilute semen and maintain sperm quality. One of the diluent for bull semen was CEP. The purpose of this study was to assess the association of bull spermatozoa quality with the physical and chemical conditions of CEP diluents with the addition of egg yolk before and after the storage process. The study used Limousin bull with 5 replications. The quality of spermatozoa included motility and viability. Physical and chemical conditions included the pH and osmolarity of the diluent. The motility of spermatozoa was observed under a light microscope with 200 X magnification at 37°C by two people. The viability of spermatozoa was observed under a light microscope with 400 X magnification with nigrosine eosin staining. Data were analyzed with ANOVA and continued Duncan’s test. Dilution pH was measured using pH indicator paper ranging from 6-8. The osmolarity of the diluent was measured by electrical osmolarity. The results showed that the addition of egg yolk in the CEP diluent decreased the pH and increased osmolartitas, but the quality of spermatozoa can be kept up to 8 days of storage. The conclusion in this study was the addition of egg yolk in the CEP diluent provided physical and chemical conditions that can maintain the quality of spermatozoa during storage at a temperature of 4-5 ° C.

  15. Quality assurance program in the External dosimetry laboratory of the CPHR

    International Nuclear Information System (INIS)

    Molina P, D.; Pernas S, R.; Martinez H, E.; Cardenas H, J.

    2006-01-01

    From 1999 the Laboratory of External Dosimetry of the Radiation Protection and Hygiene Center comes applying in its service of personal dosimetry a Program of Quality Assurance. This program was designed according to the recommendations of national and international organizations as the National Assuring Office of the Republic of Cuba (ONARC), the International Standards Organization (ISO), the International Electro technique Commission (IEC) and the International Atomic Energy Agency (IAEA). In this work it is presented in a summarized way the operation of this Program of Quality Assurance which includes the administration and conservation of the results and the documentation of the service, the controls that are carried out to the equipment, the acceptance tests that are applied to the equipment and new dosemeters, the shipment and prosecution of the dosemeters, the evaluation, storage and conservation of the doses, the report of the results, the traceability and reproducibility of the measurements, the attention to the reclamations and the clients complaints and the internal and external audits to those that it undergoes periodically the laboratory. (Author)

  16. New egg shipping container for decentralized medfly sterile male pupae production

    International Nuclear Information System (INIS)

    Kozanek, M.; Vidlicka, L.; Paskova, M.; Eyles, D.K.

    2006-01-01

    This paper describes a new double-walled container with an internal shelf system for the shipment of medfly eggs. It allows the eggs to be packed in plastic bags for shipment without the addition of any transportation medium. The size of this container can be adapted to the volume of eggs required. This container can maintain a constant internal temperature for 72 hours. Simulation of shipping conditions for 72 hours found no negative influence on the quality of eggs. The cost of the new container is comparable or even lower than the cost of commonly available commercial containers. The main advantages of this egg shipment container are: a) good thermo-insulation properties, b) variable size and c) low cost. (author)

  17. New egg shipping container for decentralized medfly sterile male pupae production

    Energy Technology Data Exchange (ETDEWEB)

    Kozanek, M.; Vidlicka, L.; Paskova, M., E-mail: milan.kozanek@savba.s, E-mail: lubomir.vidlicka@savba.s, E-mail: martina.paskova@savba.s [Slovak Academy of Sciences, Bratislava (Slovakia). Inst. of Zoology; Moravek, I., E-mail: Moravek@kvs.sif.stuba.s [Slovak Technical Univ., Bratislava (Slovakia). Fac. of Mechanical Engineering; Quintal, C., E-mail: celioquintal.sra@gov-madeira.p [Programa Madeira-Med, Madeira (Portugal); Eyles, D.K., E-mail: dke@liverpool.ac.u [The University of Liverpool, Liverpool (United Kingdom). School of Biological Sciences

    2006-07-01

    This paper describes a new double-walled container with an internal shelf system for the shipment of medfly eggs. It allows the eggs to be packed in plastic bags for shipment without the addition of any transportation medium. The size of this container can be adapted to the volume of eggs required. This container can maintain a constant internal temperature for 72 hours. Simulation of shipping conditions for 72 hours found no negative influence on the quality of eggs. The cost of the new container is comparable or even lower than the cost of commonly available commercial containers. The main advantages of this egg shipment container are: a) good thermo-insulation properties, b) variable size and c) low cost. (author)

  18. The external quality assessment scheme: Five years experience as a participating laboratory

    Directory of Open Access Journals (Sweden)

    Chaudhary Rajendra

    2010-01-01

    Full Text Available Background and Aim : Quality assurance in blood banking includes active participation in the external quality program. Such a program offers valuable benefits to patient care, their safety, and an overall quality of laboratory practices. In the year 2002, we participated in the External Quality Assessment Scheme (EQAS under the World Health Organization (WHO, Bureau of Laboratory Quality Standards, Thailand. Materials and Methods: In the current study we evaluated our EQAS test result of the past five years, from 2003 to 2007. Test results of all blood samples such as ABO grouping, D typing, antibody screening, antibody identification, and transfusion transmitted infection (TTI testing were analyzed and documented. Results: Discordant results in one or more instances were observed with antibody identification, weak D testing, and tests for anti-HIV1/2 and HBsAg. Twice we failed to detect the ′anti-Mia′ antibody in the issued sample and that could be attributed to the absence of the corresponding antigen in the used cell panel. HBsAg was missed due to its critically low titer in the serum and the comparatively low sensitivity of our Enzyme-Linked Immunosorbent Assay (ELISA test kit. Conclusion: All these failures in the last five years have helped us to significantly improve our transfusion service in terms of performance evaluation, patient care and safety issues, and the overall quality of laboratory practices. We therefore recommend all laboratories and hospitals to participate in the EQAS program, which will definitely help them to improve from what they learn.

  19. Integration: valuing stakeholder input in setting priorities for socially sustainable egg production.

    Science.gov (United States)

    Swanson, J C; Lee, Y; Thompson, P B; Bawden, R; Mench, J A

    2011-09-01

    Setting directions and goals for animal production systems requires the integration of information achieved through internal and external processes. The importance of stakeholder input in setting goals for sustainable animal production systems should not be overlooked by the agricultural animal industries. Stakeholders play an integral role in setting the course for many aspects of animal production, from influencing consumer preferences to setting public policy. The Socially Sustainable Egg Production Project (SSEP) involved the development of white papers on various aspects of egg production, followed by a stakeholder workshop to help frame the issues for the future of sustainable egg production. Representatives from the environmental, food safety, food retail, consumer, animal welfare, and the general farm and egg production sectors participated with members of the SSEP coordination team in a 1.5-d workshop to explore socially sustainable egg production. This paper reviews the published literature on values integration methodologies and the lessons learned from animal welfare assessment models. The integration method used for the SSEP stakeholder workshop and its outcome are then summarized. The method used for the SSEP stakeholder workshop can be used to obtain stakeholder input on sustainable production in other farm animal industries.

  20. Guideline on the requirements of external quality assessment programs in molecular pathology

    NARCIS (Netherlands)

    van Krieken, J Han; Normanno, Nicola; Blackhall, Fiona; Boone, Elke; Botti, Gerardo; Carneiro, Fatima; Celik, Ilhan; Ciardiello, Fortunato; Cree, Ian A; Deans, Zandra C; Edsjö, Anders; Groenen, Patricia J T A; Kamarainen, Outi; Kreipe, Hans H; Ligtenberg, Marjolijn J L; Marchetti, Antonio; Murray, Samuel; Opdam, Frank J M; Patterson, Scott D; Patton, Simon; Pinto, Carmine; Rouleau, Etienne; Schuuring, Ed; Sterck, Silke; Taron, Miquel; Tejpar, Sabine; Timens, Wim; Thunnissen, Erik; van de Ven, Peter M; Siebers, Albert G; Dequeker, Elisabeth

    Molecular pathology is an integral part of daily diagnostic pathology and used for classification of tumors, for prediction of prognosis and response to therapy, and to support treatment decisions. For these reasons, analyses in molecular pathology must be highly reliable and hence external quality

  1. Effects of parenteral gibberellic acid and dietary supplementaion of vitamin D3 on egg quality and physiological characteristics in aged laying hens

    Directory of Open Access Journals (Sweden)

    Waleed M. Razuki

    2014-12-01

    Full Text Available The aim of this study was to determine the effect of parenteral gibberellic acid (GA3 and/or vitamin D3 supplementation in diet on egg quality and blood physiological characteristics in aged laying hens. A total of 270 Lohmann Brown Classic laying hens aging 73-week were randomly assigned to equal three treatment groups (T1, T2 and T3 with equal 3 replicas in each group. The birds of group T1 (control group were injected subcutaneously (SC with sesame oil at 0.2 mL/kg body weight. The birds of group T2 were given with GA3 at 400 µg/kg b.wt., SC, whereas group T3 had diet containing vitamin D3 at 500 IU/kg feed. Relative weight of albumen and egg shell, Haugh unit, shell thickness, serum glucose, serum calcium, serum phosphorous, serum estradiol, and bone calcium absorption were significantly increased in the birds of group T2 and T3. On the other hand, relative weight of yolk, yolk cholesterol, and serum cholesterol were significantly decreased in group T2 and T3 as compared to group T1. However, serum protein and albumen were unaffected in the treatments. In conclusion, the parenteral GA3 and vitamin D3 supplementation in diet could improve egg quality traits and serum blood biochemical perperties in agend laying hens.

  2. [Analysis of the results of the SEIMC External Quality Control Program. Year 2011].

    Science.gov (United States)

    Ruiz de Gopegui Bordes, Enrique; Guna Serrano, M del Remedio; Orta Mira, Nieves; Ovies, María Rosario; Poveda, Marta; Gimeno Cardona, Concepción

    2013-02-01

    The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica [SEIMC]) includes controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology, and molecular microbiology. This article presents the most relevant conclusions and lessons from the 2011 controls. Overall, the results obtained in 2011 confirm the excellent skill and good technical standards found in previous years. Nevertheless, erroneous results can be obtained in any laboratory and in clinically relevant determinations. The results of this program highlight the need to implement both internal and external controls, such as those offered by the SEIMC program, in order to ensure maximal quality of microbiological tests. Copyright © 2013 Elsevier España, S.L. All rights reserved.

  3. Perceived external prestige as a mediator between quality of work life and organisational commitment of public sector employees in Ghana

    Directory of Open Access Journals (Sweden)

    Oluyinka Ojedokun

    2015-04-01

    Research purpose: This research seeks to close a research gap by determining the role of perceived external prestige in the relationship between quality of work life and organisational commitment amongst public sector employees in Ghana. Research approach, design and method: Theoretically guided hypotheses and models were formulated and tested with hierarchical multiple regression statistics using data from a sample of 137 employees from two public sector organisations in Ghana. Main findings: The results support the hypothesis that quality of work life is positively related to both perceived external prestige and organisational commitment. Also, perceived external prestige was found to predict organisational commitment and partially mediate the relationship between quality of work life and organisational commitment. Practical/managerial implications: The findings imply that one sure way to enhance organisational commitment of employees is by improving their quality of work life and boosting their perceptions of external prestige of the organisation. These results will be of particular interest to policymakers, public organisations and stakeholders interested in increasing organisational commitment of their employees. Contribution/value-add: The findings extend previous research by establishing the mediating role of perceived external prestige in the relationship between quality of work life and organisational commitment. If managers of organisations wish to improve organisational commitment, it is wise to institutionalise an organisational culture that promotes good quality of work life and boost the external prestige of the organisation in the employees’ mind.

  4. Observations of the "egg white injury" in ants.

    Directory of Open Access Journals (Sweden)

    Laure-Anne Poissonnier

    Full Text Available A key determinant of the relationship between diet and longevity is the balance of protein to carbohydrate in the diet. Eating excess protein relative to carbohydrate shortens lifespan in solitary and social insects. Here we explored how lifespan and behavior in ants was affected by the quality of protein ingested and the presence of associated antinutrients (i.e. compounds that interfere with the absorption of nutrients. We tested diets prepared with either egg white protein only or a protein mixture. Egg white contains an anti-nutrient called avidin. Avidin binds to the B vitamin biotin, preventing its absorption. First, we demonstrate that an egg-white diet was twice as deleterious as a protein-mixture diet. Second, we show that ingestion of egg-white diet drastically affected social behavior, triggering elevated levels of aggression within the colony. Lastly, we reveal that by adding biotin to the egg white diet we were able to lessen its detrimental effects. This latest result suggests that ants suffered biotin deficiency when fed the egg white diet. In conclusion, anti-nutrients were known to affect health and performance of animals, but this is the first study showing that anti-nutrients also lead to severe changes in behavior.

  5. Advanced Oxidation Process sanitization of hatching eggs reduces Salmonella in broiler chicks.

    Science.gov (United States)

    Rehkopf, A C; Byrd, J A; Coufal, C D; Duong, T

    2017-10-01

    The microbial quality of eggs entering the hatchery is an important critical control point for biosecurity, pathogen reduction, and food safety programs in poultry production. Developing interventions to reduce Salmonella contamination of eggs is important to improving the microbial food safety of poultry and poultry products. The hydrogen peroxide (H2O2) and ultraviolet light (UV) Advanced Oxidation Process (AOP) has been previously demonstrated to be effective in reducing Salmonella on the surface of experimentally contaminated eggs. The objective of this study was to evaluate the effect of treating eggs with an egg-sanitizing apparatus using the H2O2/UV AOP on Salmonella contamination during incubation, hatching, and in broiler chicks during grow-out. Experimentally contaminated eggs were treated using the automated H2O2/UV AOP egg sanitizer and incubated for 21 d. AOP sanitization reduced Salmonella up to 7 log10 cfu egg-1 (P Salmonella positive eggs by up to 75% (P Salmonella-positive eggs during incubation. Additionally, Salmonella was recovered from more chicks hatched from untreated eggs than from eggs treated using the H2O2/UV AOP egg sanitizer (P Salmonella contamination on the surface of eggs using the H2O2/UV AOP egg sanitizer prior to incubation may reduce the gastrointestinal colonization of chicks by Salmonella. © 2017 Poultry Science Association Inc.

  6. PRODUCTIVITY OF LAYERS AND EGG QUALITY IN FREE RANGE AND CAGE SYSTEM OF HOUSING

    OpenAIRE

    Đ. Senčić; Danijela Butko

    2006-01-01

    The research was conducted with two groups of Lohmann Brown hybrid layers. Production of eggs lasted for 52 weeks. A control group of layers was kept in the conventional housing system, that is, in cages, while experimental group was kept in the free range system. Layers from the free range system, compared to those kept in cages, laid fewer eggs, (266:295), they consumed more feed on daily basis (129 g : 115 g), more feed per kilogram of egg weight (2.83 kg : 2.35 kg), they had higher mor...

  7. Commercially laid eggs vs. discarded hatching eggs: contamination by Salmonella spp.

    Science.gov (United States)

    Kottwitz, Luciana B M; Leão, Joice Aparecida; Back, Alberto; Rodrigues, Dalia dos P; Magnani, Marciane; de Oliveira, Tereza C R M

    2013-01-01

    Salmonella enterica is frequently associated with outbreaks of human salmonellosis, and products of avian origin, such as eggs and chicken meat, are the main vehicles of its transmission. The present study describes the occurrence of different serovars of Salmonella enterica and phagotypes of S. enterica serovar Enteritidis in eggs destined for human consumption. Four thousand eggs obtained from commercial egg laying farms and one thousand discarded hatching eggs from broiler farms, which were acquired at farmers' markets and informal shops, were analyzed. Salmonella spp. was isolated from 52.0% of the discarded hatching eggs, in which the predominant serovar was Enteritidis (84.6%), and the predominant Salmonella Enteritidis phagotype (PT) was PT7 (26.9%). Salmonella spp. was not isolated from eggs obtained from commercial egg laying farms. The antimicrobial resistance profile showed that 23.1% (n = 6) of the SE strains were resistant to nalidixic acid. The results suggest that the consumption of discarded hatching eggs represents an important source of Salmonella transmission to humans.

  8. Mother and offspring fitness in an insect with maternal care: phenotypic trade-offs between egg number, egg mass and egg care

    Science.gov (United States)

    2014-01-01

    Background Oviparous females have three main options to increase their reproductive success: investing into egg number, egg mass and/or egg care. Although allocating resources to either of these three components is known to shape offspring number and size, potential trade-offs among them may have key impacts on maternal and offspring fitness. Here, we tested the occurrence of phenotypic trade-offs between egg number, egg mass and maternal expenditure on egg care in the European earwig, Forficula auricularia, an insect with pre- and post-hatching forms of maternal care. In particular, we used a series of laboratory observations and experiments to investigate whether these three components non-additively influenced offspring weight and number at hatching, and whether they were associated with potential costs to females in terms of future reproduction. Results We found negative associations between egg number and mass as well as between egg number and maternal expenditure on egg care. However, these trade-offs could only be detected after statistically correcting for female weight at egg laying. Hatchling number was not determined by single or additive effects among the three life-history traits, but instead by pairwise interactions among them. In particular, offspring number was positively associated with the number of eggs only in clutches receiving high maternal care or consisting of heavy eggs, and negatively associated with mean egg mass in clutches receiving low care. In contrast, offspring weight was positively associated with egg mass only. Finally, maternal expenditure on egg care reduced their future reproduction, but this effect was only detected when mothers were experimentally isolated from their offspring at egg hatching. Conclusions Overall, our study reveals simultaneous trade-offs between the number, mass and care of eggs. It also demonstrates that these factors interact in their impact on offspring production, and that maternal expenditure on egg

  9. Effect of dietary supplementation of hemp seed (Cannabis sativa L.) on meat quality and egg fatty acid composition of Japanese quail (Coturnix coturnix japonica).

    Science.gov (United States)

    Yalcin, H; Konca, Y; Durmuscelebi, F

    2018-02-01

    This study was conducted in two separate experiments. Experiment I was conducted to investigate the effects of hemp seed (HS) on meat quality traits, and experiment II was designed to determine egg fatty acid (FA) composition in Japanese quail (Coturnix coturnix japonica). In experiment I and experiment II treatments, groups were as follows: (i) control (not included HS0), (ii) 5% HS inclusion to diets, (iii) 10% HS inclusion to diets and (iv) 20% HS inclusion to diets. In experiment I, a total of 192 7-day-old-quail were fed with HS diets for 5 weeks, and end of the experiment I, a total of 64 quail (16 each) slaughtered and meat samples were analysed for meat colour, pH, cooking and thawing loss and FA composition. In experiment II, a total of 120 8-week-old laying quail were fed with experimental diets for 6 weeks and egg FA composition were determined. Breast meat cooking loss was significantly lower in 20% supplemented group (p egg samples were linearly increased with increasing the dietary HS ratio (p eggs (p egg yolk omega-3 FA content and decrease cooking loss of quail meats. © 2017 Blackwell Verlag GmbH.

  10. Impact of supplementing diets with propolis on productive performance, egg quality traits and some haematological variables of laying hens.

    Science.gov (United States)

    Abdel-Kareem, A A A; El-Sheikh, T M

    2017-06-01

    One hundred and twenty eight, 28-weeks-old Lohmann LSL hybrid layers were used in this experiment, which lasted 12 weeks to investigate the effect of propolis supplementation on the productive performance, egg quality traits and haematological variables of laying hens. All hens were randomly classified into four equal experimental groups, eight replicates (4 birds/each). Hens in group 1 were fed on a commercial diet and considered as control group, while those in groups 2, 3 and 4 were fed on the same commercial diet and supplemented with 250, 500 and 1000 mg propolis/kg diet. The obtained results revealed that daily feed consumption/hen increased insignificantly with increasing propolis level than that of the control group. Regarding the means of egg mass and egg production rate, it was observed that the laying hens fed diets containing 250 and 1000 mg propolis/kg significantly (p hens as compared to those in the control. Concerning the haematological parameters, the results showed that the levels of total protein and globulin increased significantly with increasing propolis level, while cholesterol and liver enzymes were significantly decreased (p hens in the treated groups significantly decreased, whereas the lymphocyte count significantly increased, resulting in a decreased H/L ratio than that of the control group. Thus, it could be concluded that the supplementation of 250 mg propolis/kg diet is highly recommended to improving egg production, blood constituent and haematological parameters of the commercial laying hens. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  11. Implementation of External Quality Assurance Trials for Immunohistochemically Determined Breast Cancer Biomarkers in Germany.

    Science.gov (United States)

    von Wasielewski, Reinhard; Krusche, Claudia A; Rüschoff, Joseph; Fisseler-Eckhoff, Anette; Kreipe, Hans

    2008-01-01

    Besides typing and grading of breast cancer, Pathologists are involved in the determination of biomarkers, such as steroid hormone receptors and HER2, which are of utmost importance in adjuvant therapy. There have been concerns with regard to security and reproducibility of the biomarker assays done on tissue sections applying either immunohistochemistry or in-situ hybridisation. In order to assure the quality of these biomarker assays, a number of measures are required, among them external proficiency testing. Therefore, external quality assurance trials have been implemented in Germany. In the period of 2002-2007, 5 consecutive trials were conducted with up to 180 participating laboratories. Tissue microarrays with 20-24 different breast cancer samples including cell lines enabled that a huge number of pathologists were challenged with identical samples which provides the prerequisite for comparability. Because there is no legal duress to undergo external proficiency testing in histopathology, all laboratories that took part volunteered to do so. These innovative quality assurance trials (Qualitätsinitiative Pathologie, QuIP) will be continued in the future on an annual or bi-annual basis. Participation is recommended for pathology departments involved in the service for breast units. The organisational frame work of the trials is described here.

  12. Effect of feeding CLA on plasma and granules fatty acid composition of eggs and prepared mayonnaise quality.

    Science.gov (United States)

    Shinn, Sara Elizabeth; Proctor, Andrew; Gilley, Alex D; Cho, Sungeun; Martin, Elizabeth; Anthony, Nicholas B

    2016-04-15

    Eggs rich in trans, trans conjugated linoleic acid (CLA) are significantly more viscous, have more phospholipids containing linoleic acid (LA), and more saturated triacylglycerol species than control eggs. However, the fatty acid (FA) composition of yolk plasma and granule fractions are unreported. Furthermore, there are no reports of mayonnaise rheological properties or emulsion stability by using CLA-rich eggs. Therefore, the objectives were (1) compare the FA composition of CLA-rich yolk granules and plasma, relative to standard control and LA-rich control yolks, (2) compare the rheological properties of mayonnaise prepared with CLA-rich eggs to control eggs and (3) compare the emulsion stability of CLA-yolk mayonnaise. CLA-rich eggs and soy control eggs were produced by adding 10% CLA-rich soy oil or 10% of control unmodified soy oil to the hen's diet. The eggs were used in subsequent mayonnaise preparation. CLA-yolk mayonnaise was more viscous, had greater storage modulus, resisted thinning, and was a more stable emulsion, relative to mayonnaise prepared with control yolks or soy control yolks. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. NAA Comparison of Nutriens in Egg Yolk and Egg White

    International Nuclear Information System (INIS)

    Ruangdit, S.; Maijan, P.; Channuie, J.; Picha, R.

    2014-01-01

    Food we eat has significant effects on our wellbeing. Eggs are among food products widely consumed and contain many essential nutrients. Yet eggs often are involved in dietary controversy regarding benefit versus risk. The fear of serum cholesterol has driven a large number of people away from consuming egg yolks. In this study, we assessed the nutritional importance of eggs using instrumental neutron activation analysis (INAA) technique at Thai Research Reactor, operating at 1.2 MW. We investigated whether consumers miss any health benefits by choosing to eat only egg whites or yolks rather than whole natural eggs. Essential minerals such as sodium, potassium, manganese, magnesium, copper, selenium, iron and zinc are studied using three NAA loading methods: short-term individual pneumatic transfer system loading using inner-core tubes, medium-term CA3 loading and long-term Lazy Susan (LS) loading for 10 s, 7 h and 3 d, respectively. Two Standard Reference Materials (SRMs) were used to find the nutrient concentrations and validate the method. From the study, we found significant differences in nutritional contents between egg yolks and egg whites. Along with literature review conducted as part of this study, we evaluated the consumption choice when it comes to eggs.

  14. Dinosaur origin of egg color: oviraptors laid blue-green eggs.

    Science.gov (United States)

    Wiemann, Jasmina; Yang, Tzu-Ruei; Sander, Philipp N; Schneider, Marion; Engeser, Marianne; Kath-Schorr, Stephanie; Müller, Christa E; Sander, P Martin

    2017-01-01

    Protoporphyrin (PP) and biliverdin (BV) give rise to the enormous diversity in avian egg coloration. Egg color serves several ecological purposes, including post-mating signaling and camouflage. Egg camouflage represents a major character of open-nesting birds which accomplish protection of their unhatched offspring against visually oriented predators by cryptic egg coloration. Cryptic coloration evolved to match the predominant shades of color found in the nesting environment. Such a selection pressure for the evolution of colored or cryptic eggs should be present in all open nesting birds and relatives. Many birds are open-nesting, but protect their eggs by continuous brooding, and thus exhibit no or minimal eggshell pigmentation. Their closest extant relatives, crocodiles, protect their eggs by burial and have unpigmented eggs. This phylogenetic pattern led to the assumption that colored eggs evolved within crown birds. The mosaic evolution of supposedly avian traits in non-avian theropod dinosaurs, however, such as the supposed evolution of partially open nesting behavior in oviraptorids, argues against this long-established theory. Using a double-checking liquid chromatography ESI-Q-TOF mass spectrometry routine, we traced the origin of colored eggs to their non-avian dinosaur ancestors by providing the first record of the avian eggshell pigments protoporphyrin and biliverdin in the eggshells of Late Cretaceous oviraptorid dinosaurs. The eggshell parataxon Macroolithus yaotunensis can be assigned to the oviraptor Heyuannia huangi based on exceptionally preserved, late developmental stage embryo remains. The analyzed eggshells are from three Late Cretaceous fluvial deposits ranging from eastern to southernmost China. Reevaluation of these taphonomic settings, and a consideration of patterns in the porosity of completely preserved eggs support an at least partially open nesting behavior for oviraptorosaurs. Such a nest arrangement corresponds with our

  15. Dinosaur origin of egg color: oviraptors laid blue-green eggs

    Directory of Open Access Journals (Sweden)

    Jasmina Wiemann

    2017-08-01

    Full Text Available Protoporphyrin (PP and biliverdin (BV give rise to the enormous diversity in avian egg coloration. Egg color serves several ecological purposes, including post-mating signaling and camouflage. Egg camouflage represents a major character of open-nesting birds which accomplish protection of their unhatched offspring against visually oriented predators by cryptic egg coloration. Cryptic coloration evolved to match the predominant shades of color found in the nesting environment. Such a selection pressure for the evolution of colored or cryptic eggs should be present in all open nesting birds and relatives. Many birds are open-nesting, but protect their eggs by continuous brooding, and thus exhibit no or minimal eggshell pigmentation. Their closest extant relatives, crocodiles, protect their eggs by burial and have unpigmented eggs. This phylogenetic pattern led to the assumption that colored eggs evolved within crown birds. The mosaic evolution of supposedly avian traits in non-avian theropod dinosaurs, however, such as the supposed evolution of partially open nesting behavior in oviraptorids, argues against this long-established theory. Using a double-checking liquid chromatography ESI-Q-TOF mass spectrometry routine, we traced the origin of colored eggs to their non-avian dinosaur ancestors by providing the first record of the avian eggshell pigments protoporphyrin and biliverdin in the eggshells of Late Cretaceous oviraptorid dinosaurs. The eggshell parataxon Macroolithus yaotunensis can be assigned to the oviraptor Heyuannia huangi based on exceptionally preserved, late developmental stage embryo remains. The analyzed eggshells are from three Late Cretaceous fluvial deposits ranging from eastern to southernmost China. Reevaluation of these taphonomic settings, and a consideration of patterns in the porosity of completely preserved eggs support an at least partially open nesting behavior for oviraptorosaurs. Such a nest arrangement corresponds

  16. Hoopoes color their eggs with antimicrobial uropygial secretions

    Science.gov (United States)

    Soler, Juan J.; Martín-Vivaldi, M.; Peralta-Sánchez, J. M.; Arco, L.; Juárez-García-Pelayo, N.

    2014-09-01

    Uropygial gland secretions are used as cosmetics by some species of birds to color and enhance properties of feathers and teguments, which may signal individual quality. Uropygial secretions also reach eggshells during incubation and, therefore, may influence the coloration of birds' eggs, a trait that has attracted the attention of evolutionary biologists for more than one century. The color of hoopoe eggs typically changes along incubation, from bluish-gray to greenish-brown. Here, we test experimentally the hypothesis that dark uropygial secretion of females is responsible for such drastic color change. Moreover, since uropygial secretion of hoopoes has antimicrobial properties, we also explore the association between color and antimicrobial activity of the uropygial secretion of females. We found that eggs stayed bluish-gray in nests where female access to the uropygial secretion was experimentally blocked. Furthermore, experimental eggs that were maintained in incubators and manually smeared with uropygial secretion experienced similar color changes that naturally incubated eggs did, while control eggs that were not in contact with the secretions did not experience such color changes. All these results strongly support the hypothesis that female hoopoes use their uropygial gland secretion to color the eggs. Moreover, saturation of the uropygial secretion was associated with antimicrobial activity against Bacillus licheniformis. Given the known antimicrobial potential of uropygial secretions of birds, this finding opens the possibility that in scenarios of sexual selection, hoopoes in particular and birds in general signal antimicrobial properties of their uropygial secretion by mean of changes in egg coloration along incubation.

  17. [Preservation of live eggs of Schistosoma japonicum].

    Science.gov (United States)

    Lan, Wei-ming; Xie, Shu-ying; Wang, Qin; Jiang, Wei-sheng; Hu, Ren-mei; Ge, Jun; Zeng, Xiao-jun

    2015-10-01

    To observe the preservation time and activity of miracidium hatching from schistosome eggs preserved in different solutions, so as to obtain the optimal preservation conditions and then provide quality control products for field application. The rectum stool of rabbits infected with Schistosoma japonicum was collected and the coarse fecal residue was removed with a series of sample sieves of 80, 100, 160 and 200 meshes respectively, and then the schistosome eggs were concentrated with the sample sieve of 260 meshes. The concentrated eggs were preserved in 0.9% sodium chloride solution, 1.2% sodium chloride solution, phosphate buffered saline solution (PBS, PH 7.2), 1.0% sucrose solution, and Mili-Q water, respectively, and then were conserved in a 4 °C refrigerator and 15 °C constant temperature incubator, respectively. The preserved eggs were hatched in different time (7-day interval) , the vitality and quantity of the miracidia were observed, and the hatching rates were calculated. Under the condition of 4 °C, the hatching rates of eggs dropped to 0 in 1.0% sucrose solution and 1.2% sodium chloride solution at the 49th and 126th day, respectively, and the hatching rates of eggs in the 0.9% sodium chloride solution and PBS solution dropped to 10% at the 112th day, and the activity of miracidium was weakened since 140th. In the Mili-Q water, the hatching rate dropped less than 10% at the 196th day and the activity of miracidia was weakened since the 280th day. Under the condition of 15 °C, the hatching rate of eggs in different solutions gradually dropped to 0 from the 49th day to 105th day. The eggs preserved in Mili-Q water at the temperature of 4 °C can be used as the positive reference for hatching tests within 196 days.

  18. Study on the sterilization of egg white powder by irradiation

    International Nuclear Information System (INIS)

    Fu Lixin; Meng Lifen; Zhao Xiaonan; Xu Dechun; Hu Shaoxin; Chen Changdong; Pei Ying

    2012-01-01

    The article studied the killing microorganism result, the influence of main essential nutrient and sense organ of egg white powder by Co-60 γ-ray irradiation. The microorganism index of the egg white powder samples can meet the government food sanitation standard by 4 kGy irradiation, and the killing microorganism rare can reach 100%. The irradiation dose from 4 to 10 kGy has little influence to the egg white powder protein, crude fiber and amino acids contras ting to comparison, and the sensory quality such as color and luster, scent has little change below 6 kGy irradiation. Synthesizing the results of physical chemistry and sense organ, the optimal irradiation dose of egg white powder killing microorganism is 4 to 6 kGy. (authors)

  19. Study on the sterilization of egg white powder by irradiation

    International Nuclear Information System (INIS)

    Fu Lixin; Meng Lifen; Zhao Xiaonan; Xu Dechun; Hu Shaoxin; Chen Changdong; Pei Ying

    2011-01-01

    The article studied the killing microorganism result, the influence of main essential nutrient and sense organ of egg white powder by Co-60 γ-ray irradiation. The microorganism index of the egg white powder samples can meet the government food 1 sanitation standard by 4 kGy irradiation, and the killing microorganism rare can reach 100%. The irradiation dose from 4 to 10 kGy has little influence to the egg white powder protein, crude fiber and amino acids contrasting to comparison, and the sensory quality such as color and luster, scent has little change below 6 kGy irradiation. Compositing the result of physical chemistry and sense organ, the optimal irradiation dose of egg white powder killing microorganism is 4 to 6 kGy. (authors)

  20. Egg freezing and egg banking: empowerment and alienation in assisted reproduction.

    Science.gov (United States)

    Robertson, John A

    2014-06-01

    With the development of rapid freezing of human oocytes, many programs have reported IVF success rates comparable to those achieved with fresh eggs and thawed frozen embryos. Egg freezing is now gaining professional and regulatory acceptance as a safe and effective technique for women who wish to avoid discarding excess embryos, who face fertility-threatening medical treatments, or who want to preserve their eggs for use when they are better situated to have a family. This article focuses on the uses of and justification for egg freezing, the path to professional acceptance, the variability in success rates, and the controversy over freezing eggs for social rather than medical reasons. It also addresses the emergence of egg banking as a separate sector in the infertility industry, the regulatory issues that it poses, and its effect on egg donation. Key here is the legal control of stored eggs by banking women and their options when they wish to dispose of those eggs. The analysis is framed around empowerment and alienation. Egg freezing is generally empowering for women, but the donation or sale of unused eggs to infertile women, egg bankers, and researchers also raises issues of alienation.

  1. Egg consumption, cardiovascular diseases and type 2 diabetes

    DEFF Research Database (Denmark)

    Geiker, Nina Rica Wium; Lytken Larsen, Mogens; Dyerberg, Jørn

    2018-01-01

    Eggs are rich in nutrients and a source of essential fatty- and amino acids, and the food item with highest cholesterol content. Since the 1970s dietary recommendations have advised limiting egg intake to 2-4 a week for the healthy population, and in those diagnosed with cardiovascular disease (CVD......) and type 2 diabetes (T2D) an even more restricted consumption. The aim of the present paper was to assess the recommendation to lower the dietary intake of cholesterol and especially the intake of egg to reduce the risk of CVD and T2D. We performed three web-based literature searches on human studies...... (observational and interventional) published within the past 10 years during spring 2015. High-quality intervention studies have found nonsignificant effects of increasing the consumption of eggs on risk markers for CVD and T2D in healthy subjects and subjects with T2D. The risk associations found...

  2. Challenges in Radiofrequency Pasteurization of Shell Eggs: Coagulation Rings.

    Science.gov (United States)

    Lau, Soon Kiat; Thippareddi, Harshavardhan; Jones, David; Negahban, Mehrdad; Subbiah, Jeyamkondan

    2016-10-01

    A total of 50 different configurations of simple radiofrequency (RF) heating at 27.12 MHz of a shell egg were simulated using a finite element model with the purpose of pasteurizing the egg. Temperature-dependent thermal and dielectric properties of the yolk, albumen, and shell were measured, fitted, and introduced into the model. A regression equation that relates the top electrode voltage to the gap between the electrodes and vertical position of the egg was developed. Simulation and experimental results had good agreement in terms of temperature deviation (root mean squared error ranged from 0.35 °C to 0.48 °C) and both results demonstrated the development of a "coagulation ring" around the air cell. The focused heating near the air cell of the egg prevented pasteurization of the egg due to its impact on quality (coagulation). Analysis of the electric field patterns offered a perspective on how nonuniform RF heating could occur in heterogeneous food products. The results can be used to guide development of RF heating for heterogeneous food products and further development of RF pasteurization of eggs. © 2016 Institute of Food Technologists®.

  3. Effect of dietary energy and protein on the performance, egg quality, bone mineral density, blood properties and yolk fatty acid composition of organic laying hens

    Directory of Open Access Journals (Sweden)

    Md. Rakibul Hassan

    2013-02-01

    Full Text Available An experiment was conducted to evaluate the effect of dietary metabolizable energy (ME and crude protein (CP on the performance, egg quality, blood properties, bone characteristics and yolk fatty acid composition of organic laying hens. At 23 weeks, a total of 600 Brown nick laying hens were randomly distributed into 24 outdoor pens (4 replicate pens/treatment; 25 birds/pen and were given (2750, 2775 and 2800 kcal of ME/kg and CP (16 and 17% resulting in a 3×2 factorial arrangement of organic dietary treatments. The experiment lasted 23 weeks. The performance of laying hens were not affected by the dietary treatment while the egg weight was increased with energy and CP levels in the diet (P<0.05. Serum total protein was not affected by dietary energy and protein level. Total cholesterol and triglyceride tend to reduce with the increasing amount of CP in the diet. Thereafter, bone and egg quality characteristics were numerically increased in dietary 2775 kcal of ME/kg and 16% CP treatment. On the other hand, docosahexanoic acid content in egg yolk was higher (P<0.01 in 2750 kcal of ME/kg and 17% CP treatment. As a result, the performance, blood and fatty acid composition were maximized in 2750 kcal of ME/kg and 16% CP treatment. Thus, dietary 2750-2775 kcal of ME/kg and 16% CP may enhance performance, blood and fatty acid composition of organic laying hens.

  4. Egg-laying sequence influences egg mercury concentrations and egg size in three bird species: Implications for contaminant monitoring programs.

    Science.gov (United States)

    Ackerman, Joshua T; Eagles-Smith, Collin A; Herzog, Mark P; Yee, Julie L; Hartman, C Alex

    2016-06-01

    Bird eggs are commonly used in contaminant monitoring programs and toxicological risk assessments, but intraclutch variation and sampling methodology could influence interpretability. The authors examined the influence of egg-laying sequence on egg mercury concentrations and burdens in American avocets, black-necked stilts, and Forster's terns. The average decline in mercury concentrations between the first and last eggs laid was 33% for stilts, 22% for terns, and 11% for avocets, and most of this decline occurred between the first and second eggs laid (24% for stilts, 18% for terns, and 9% for avocets). Trends in egg size with egg-laying order were inconsistent among species, and overall differences in egg volume, mass, length, and width were eggs laid. Despite the strong effect of egg-laying sequence, most of the variance in egg mercury concentrations still occurred among clutches (75-91%) rather than within clutches (9%-25%). Using simulations, the authors determined that accurate estimation of a population's mean egg mercury concentration using only a single random egg from a subset of nests would require sampling >60 nests to represent a large population (10% accuracy) or ≥14 nests to represent a small colony that contained <100 nests (20% accuracy). Environ Toxicol Chem 2016;35:1458-1469. Published 2015 Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the United States of America. Published 2015 Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the United States of America.

  5. Effects of the tricothecene mycotoxin diacetoxyscirpenol on egg production of broiler breeders.

    Science.gov (United States)

    Brake, J; Hamilton, P B; Kittrell, R S

    2002-12-01

    Three experiments were conducted to determine the effects of 4,15-diacetoxyscirpenol (DAS) on egg quality and egg production of broiler breeders. In Experiment 1, feed containing 0, 1.25, 2.5, or 5.0 mg DAS/ kg was fed from 67 to 69 wk of age followed by a 3-wk recovery period on a slat-litter floor. In Experiment 2, individually caged broiler breeder females were studied from 23 to 31 wk of age. The basal diet containing 0, 5, 10, or 20 mg DAS/kg was fed from 25 to 27 wk of age. In Experiment 3, individually caged broiler breeder hens were studied from 23 to 32 wk of age. DAS was fed at levels of 0 (basal), 5, 10, and 20 mg DAS/kg for 2 wk beginning at Week 24, followed by the basal breeder diet for 7 wk. Egg production was not affected by levels of up to 5 mg DAS/kg in the older hens of Experiment 1. When fed from 25 to 27 wk of age in Experiment 2, DAS decreased egg production at the 20 mg/kg level only. When fed from 24 to 25 wk of age in Experiment 3, DAS had no significant effect on egg production or egg quality. Short-term consumption of DAS at levels that might naturally occur appears to have little effect on broiler breeder egg production.

  6. Effects of feeding transgenic corn with mCry1Ac or maroACC gene to laying hens for 12 weeks on growth, egg quality and organ health.

    Science.gov (United States)

    Zhong, R Q; Chen, L; Gao, L X; Zhang, L L; Yao, B; Yang, X G; Zhang, H F

    2016-08-01

    The objective of the present study was to investigate the effect of feeding two transgenic corn lines containing the mCry1Ac gene from Bacillus thuringiensis strain (BT-799) and the maroACC gene from Agrobacterium tumefaciens strain (CC-2), respectively, on growth, egg quality and organ health indicators. Expression of the mCry1Ac gene confers resistance to Pyrausta nubilalis and the maroACC gene confers tolerance to herbicides. Healthy hens (n=96 placed in cages; 3 hens/cage) were randomly assigned to one of four corn-soybean meal dietary treatments (8 cages/treatment) formulated with the following corn: non-transgenic near-isoline control corn (control), BT-799 corn, CC-2 corn and commercially available non-transgenic reference corn (reference). The experiment was divided into three 4-week phases (week 1 to 4, week 5 to 8 and week 9 to 12), during which hens were fed mash diets. Performance (BW, feed intake and egg production) and egg quality were determined. Following slaughter at the end of 12 weeks of feeding (n=8/treatment), carcass yield and organ weights (heart, liver, spleen, lung, kidneys, stomach and ovary) were recorded; organs and intestines were sampled for histological analysis. Analysis of serum biochemistry parameters to assess the liver and kidney function were performed. No differences in BW, egg production and production efficiency were observed between hens consuming the control diet and hens consuming the BT-799 or CC-2 diet. Haugh unit measures and egg component weights were similar between the control and test groups. Carcass yield was not affected by the diet treatment. Similar organosomatic indices and serum parameters did not indicate the characteristics of organ dysfunction. All observed values of the BT-799 and CC-2 groups were within the calculated tolerance intervals. This research indicates that the performance, egg quality, organ health and carcass yield of laying hens fed diets containing the BT-799 or CC-2 corn line were similar

  7. Effects of Cassava (Manihot Esculenta Crantz Root Meal in Diets Containing Corn Dried Distillers Grains With Solubles on Production Performance, Egg Quality, and Excreta Noxious Gas Emission in Laying Hens

    Directory of Open Access Journals (Sweden)

    XJ Lei

    Full Text Available ABSTRACT This study was conducted to evaluate effects of cassava (Manihot esculenta Crantz root meal (CRM in laying hen diets containing corn dried distiller grains with soluble (DDGS on production performance, egg quality, and excreta noxious gas emission. Two hundred and forty Hy-Line brown laying hens (40 weeks of age were randomly divided into 1 of 4 dietary treatments (10 replications with 6 hens per replication for 6 weeks. The dietary treatments were as follows: 1 corn-based diet (CON; 2 diet containing 10% CRM and 8% DDGS (CRM10; 3 diet containing 20% CRM and 8% DDGS (CRM20; 4 diet containing 30% CRM and 8% DDGS (CRM30. The inclusion of 30% CRM in the diet containing 8% DDGS significantly decreased (p0.05 the egg quality with the exception of decreased (p<0.05 egg yolk color when 30% of CRM was included in laying hens diet. CRM20 and CRM30 dietary treatments tended to decrease ammonia emission compared with CON dietary treatment (p=0.08. In conclusion, the results of the current study demonstrated that CRM may be incorporated to a concentration of 20% in laying hen diets containing 8% DDGS without detrimental effects on production performance and egg quality. Furthermore, the addition of 20% and 30% CRM in laying hen diets containing 8% DDGS tended to reduce the excreta ammonia emission.

  8. Antiproliferative Activity of Egg Yolk Peptides in Human Colon Cancer Cells.

    Science.gov (United States)

    Yousr, Marwa N; Aloqbi, Akram A; Omar, Ulfat M; Howell, Nazlin K

    2017-01-01

    Egg yolk peptides were successfully prepared from egg yolk protein by-products after lecithin extraction. Defatted egg yolk protein was hydrolyzed with pepsin and pancreatin and purified by gel filtration to produce egg yolk gel filtration fraction (EYGF-33) with antiproliferative activity. The highlight of this study was that the peptide EYGF-33 (1.0 mg/ml) significantly inhibits cell viability of colon cancer cells (Caco-2) with no inhibitory effects on the viability of human colon epithelial normal cells (HCEC) after 48 h. Reduced cell viability can be explained by cell cycle arrest in the S-phase in which DNA replication normally takes place. EYGF-33 significantly enhanced the production of superoxide anions in the mitochondria of Caco-2 cells; this could activate a mitochondrial apoptotic pathway leading to typical Poly Adenosine diphosphate-ribose polymerase (PARP) cleavage as observed in the Western blot result. The induction of apoptotic cell death by EYGF-33 was supported by the externalization of phosphatidylserine (PS). However, further elucidation of the mechanism of EYGF-33-mediated apoptosis would provide further support for its use as a potential therapeutic and chemopreventive agent.

  9. The table egg: a review Ovo de consumo: uma revisão

    Directory of Open Access Journals (Sweden)

    Antonio Gilberto Bertechini

    2013-04-01

    Full Text Available This present review intends to provide information and update the reader about diverse aspects of quality and consumption of eggs, the variation among consumer preference worldwide emphasizing the upper status this food represents as source of essential nutrients for human health. This review mainly focus on topics regarding production and quality of table eggs, emphasizing some aspects of the egg market, trend in egg intake and consumers perceptions regarding egg content and nutritious values. Additionally, results from specific studies that provide new findings about the role of enrichment egg components on health, prevention and treatment of diseases are discussed. A novel viewpoint is proposed in this review, highlighting the perspective for an increase of consumption of egg and egg products, mainly in markets where the consumption pattern is known to be low. How the information about shell eggs and its nutrients reach the consumer strongly affect the way this food of higher biological functions takes part in the human diet.Nesta revisão, os autores buscam informar sobre os vários aspectos relacionados à qualidade e ao consumo de ovos ao nível mundial, reportando as diferenças que existem entre os países e a importância que esse alimento representa para o fornecimento de nutrientes essenciais para nutrir e manter a saúde humana. Ao mesmo tempo, a revisão enfoca os estudos específicos, que indicam as novas descobertas sobre o enriquecimento dos ovos e sua ação auxiliar, no controle de problemas de saúde humana. Existe a perspectiva de incrementos no nível de consumo, principalmente nos países onde a ingestão é baixa e, assim, melhorar a nutrição humana com esse alimento de baixo custo e, no entanto, de alta qualidade nutricional.

  10. Dinosaur origin of egg color: oviraptors laid blue-green eggs

    OpenAIRE

    Wiemann, Jasmina; Yang, Tzu-Ruei; Sander, Philipp N.; Schneider, Marion; Engeser, Marianne; Kath-Schorr, Stephanie; M?ller, Christa E.; Sander, P. Martin

    2017-01-01

    Protoporphyrin (PP) and biliverdin (BV) give rise to the enormous diversity in avian egg coloration. Egg color serves several ecological purposes, including post-mating signaling and camouflage. Egg camouflage represents a major character of open-nesting birds which accomplish protection of their unhatched offspring against visually oriented predators by cryptic egg coloration. Cryptic coloration evolved to match the predominant shades of color found in the nesting environment. Such a selecti...

  11. Comparative Effect of Dietary Soybean Oil, Fish Oil, and Coconut Oil on Performance, Egg Quality and Some Blood Parameters in Laying Hens.

    Science.gov (United States)

    Dong, X F; Liu, S; Tong, J M

    2018-04-14

    Two hundred and sixteen 28-wk-old Hy-line laying hens were randomly distributed to three dietary treatments and fed 1of 3 diets containing 8% soybean oil, fish oil, or coconut oil from 28 to 47 wk of age to investigate comparative effect of dietary soybean oil, fish oil, and coconut oil on the performance, egg quality and blood malondialdehyde (MDA), aspartate transaminase (AST) and uric acid (UA). Hens fed fish oil showed poor performance compared with soybean oil or coconut oil, and especially egg weight throughout the trial was significantly and consistently decreased (P oil. Unexpectedly, shell reflectivity throughout the majority of the trial was consistently and significantly higher (P oil than that when fed soybean oil or coconut oil. Dietary treatments affected (P oil treatment was higher (P oil group. Albumen height, Haugh unit and yolk color were influenced by dietary treatments only at 1 or 2 time points. However, average albumen height and Haugh unit in fish oil treatment were higher (P soybean oil or coconut oil treatments and average yolk color in coconut oil treatment was higher (P soybean oil group. Serum MDA, AST and UA concentrations were increased (P oil during the majority of the first 2 mo of the trial. These data suggested that the inclusion of fish oil into feed may reduce the performance of laying hens, especially the egg weight, decrease the intensity of egg brown color and increase blood MDA, AST and UA levels compared with soybean oil or coconut oil. As a result, hens fed fish oil may lay smaller, longer and lighter-brown eggs whereas those fed coconut oil produce blunter and darker-brown eggs relative to soybean oil.

  12. Effect of feed grinding methods with and without expansion on prececal and total tract mineral digestibility as well as on interior and exterior egg quality in laying hens.

    Science.gov (United States)

    Hafeez, A; Mader, A; Ruhnke, I; Männer, K; Zentek, J

    2016-01-01

    The grinding of cereals by various milling methods as well as thermal treatment of feed may influence mineral digestibility and egg quality. The present study investigated the effect of feed produced by disc mill (D) and wedge-shaped disc mill (WSD), as mash (M) or expandate (E) on apparent ileal absorption (AIA) and apparent total digestibility (ATD) of calcium, phosphorus, magnesium, zinc, manganese, copper and iron, as well as on egg quality in laying hens. A total of 192 hens (Lohmann Brown) aged 19 wk, were assigned using a randomized design with a 2 × 2 factorial arrangement. Four experimental diets were offered ad libitum. Eggs were analyzed for weight, shape index, area, shell weight per unit surface area, yolk color, air cell, blood spot, Haugh unit, albumen and yolk measures (index, weight, height, width and length), shell measures (surface area, stability, density, thickness and membrane weight), as well as percent contents of albumen, yolk, shell, and shell membrane. The ATD for phosphorus, manganese, and copper was higher in WSD compared with D treatment (P = 0.028, P = 0.028 and P = 0.016, respectively). The interaction between milling methods and thermal treatment influenced ATD of copper (P = 0.033), which was higher in WSD+M group (41.0 ± 20.2) compared with D+E group (-3.21 ± 25.1), whereas no differences were observed for D+M (1.90 ± 37.8) and WSD+E (8.02 ± 36.2) groups. Egg stability tended to be higher in E compared with M treatment (P = 0.055). Albumen weight, percentage albumen weight, and albumen: yolk were higher and percentage yolk weight was lower in D compared with WSD treatment (P = 0.043, P = 0.027, P = 0.024, and P = 0.041, respectively). Number of blood spots was higher in E than M treatment (P = 0.053). In conclusion, use of a wedge-shaped disc mill resulted in higher ATD for phosphorus, manganese, and copper than use of a disc mill; however, digestibility for majority of minerals as well as egg quality parameters was

  13. Diet shifts during egg laying: Implications for measuring contaminants in bird eggs

    Energy Technology Data Exchange (ETDEWEB)

    Morrissey, Christy A. [Catchment Research Group, School of Biosciences, Cardiff University, Cardiff CF10 3AX (United Kingdom); Elliott, John E. [Pacific Wildlife Research Centre, Environment Canada, 5421 Robertson Road, Delta, British Columbia V4K 3N2 (Canada); Ormerod, Stephen J., E-mail: ormerod@cf.ac.u [Catchment Research Group, School of Biosciences, Cardiff University, Cardiff CF10 3AX (United Kingdom)

    2010-02-15

    We combined stable isotope tracers of blood plasma, blood cells and egg contents with faecal analysis during pre-breeding and egg laying phases in two dipper species Cinclus cinclus and Cinclus mexicanus to determine the occurrence of dietary shifts during egg production and to assess consequences for egg contaminant loads. In both species, changes in delta{sup 13}C (C. cinclus) or delta{sup 15}N (C. mexicanus) in female plasma relative to red blood cells indicated a dietary shift during laying that was not observed in males. Eurasian dippers increased prey consumption as breeding approached, shifting from primarily trichopteran insect larvae to ephemeropterans and plecopterans. In American dippers, egg-laying females switched to feeding at a higher trophic level by consuming more fish. Eggs derived from higher trophic level diets contained more mercury (American dipper), polychlorinated biphenyls and some organochlorines, especially DDT metabolites. The results demonstrate how dietary changes during egg laying accompany the demands for egg production with consequences for contaminant deposition in avian eggs. - Changes in laying diet influences contaminant deposition in bird eggs.

  14. Diet shifts during egg laying: Implications for measuring contaminants in bird eggs

    International Nuclear Information System (INIS)

    Morrissey, Christy A.; Elliott, John E.; Ormerod, Stephen J.

    2010-01-01

    We combined stable isotope tracers of blood plasma, blood cells and egg contents with faecal analysis during pre-breeding and egg laying phases in two dipper species Cinclus cinclus and Cinclus mexicanus to determine the occurrence of dietary shifts during egg production and to assess consequences for egg contaminant loads. In both species, changes in δ 13 C (C. cinclus) or δ 15 N (C. mexicanus) in female plasma relative to red blood cells indicated a dietary shift during laying that was not observed in males. Eurasian dippers increased prey consumption as breeding approached, shifting from primarily trichopteran insect larvae to ephemeropterans and plecopterans. In American dippers, egg-laying females switched to feeding at a higher trophic level by consuming more fish. Eggs derived from higher trophic level diets contained more mercury (American dipper), polychlorinated biphenyls and some organochlorines, especially DDT metabolites. The results demonstrate how dietary changes during egg laying accompany the demands for egg production with consequences for contaminant deposition in avian eggs. - Changes in laying diet influences contaminant deposition in bird eggs.

  15. Study on preparation the egg yolk puff with chitosan

    Directory of Open Access Journals (Sweden)

    LI Hui

    2014-12-01

    Full Text Available This paper was studied chitosans with different degrees of deacetylation (70%,80%,90%,95% and different usages of chitosan that were added to research the effect of functional indexs in the egg yolk puff,such as calcium content and cholesterol content.Preliminarily chitosan was explored in the application of the Egg yolk puff.Text results showed that when the deacetylation degree of chitosan and its usage were 90% and 1% separately,the functional indexs and sensory quality of the Egg yolk puff can reach the equilibrium.Its calcium content was 76.2 mg/100 g,increased by 44.3 percent.Its cholesterol content was 290 mg/100 g,decreased by 35.1%.

  16. Assessing the impact of egg sweating on Salmonella Enteritidis penetration into shell eggs.

    Science.gov (United States)

    Gradl, Janet A; Curtis, Patricia A; Jones, Deana R; Anderson, Kenneth E

    2017-07-01

    Salmonella Enteritidis (SE) prevalence in eggs is a major concern to the egg industry. Some research has shown that egg sweating can increase Salmonella penetration into egg contents when refrigerated eggs are moved to a warmer temperature. This occurs when eggs are tempered before wash, to minimize thermal cracks. The effect of egg sweating on SE penetration into shell eggs over a 6 week storage period at 4°C was assessed. A 2 × 2 factorial of SE inoculation and egg sweating was utilized. Treatments included (SES) nalidixic acid (NA)-resistant SE inoculated and sweated, (SENS) NA-resistant SE inoculated and not sweated, (NSES) buffered peptone water (BPW) inoculated and sweated, and (NSENS) BPW inoculated and not sweated. Eggs were inoculated with 108 SE. Eggs formed condensation for approximately 17 min in a 32°C incubator. Shell rinse, shell emulsion, and egg contents were sampled then enumerated and assessed for prevalence of SE over a 6 wk storage period at 4°C. After wk 1, the SENS shell rinse had higher SE counts (0.32 log10 CFU/mL) than the other 3 treatments, where no SE was enumerated. A significant week by treatment interaction was found for the shell rinse SE detection (P egg shell rinse, shell emulsion, or egg contents. The SENS shell rinses had significantly higher SE prevalence than the SES rinses in weeks 1 (100% vs. 34.3%), 2 (57.6% vs. 22.2%), and 3 (38.2% vs. 11.1%) (P Egg sweating did not increase SE penetration into the shell emulsion across treatment or week (P egg sweating occurring under common US egg handling practices is not harmful to egg safety. © 2017 Poultry Science Association Inc.

  17. Lack of grading agreement among international hemostasis external quality assessment programs.

    Science.gov (United States)

    Olson, John D; Jennings, Ian; Meijer, Piet; Bon, Chantal; Bonar, Roslyn; Favaloro, Emmanuel J; Higgins, Russell A; Keeney, Michael; Mammen, Joy; Marlar, Richard A; Meley, Roland; Nair, Sukesh C; Nichols, William L; Raby, Anne; Reverter, Joan C; Srivastava, Alok; Walker, Isobel

    2018-01-01

    : Laboratory quality programs rely on internal quality control and external quality assessment (EQA). EQA programs provide unknown specimens for the laboratory to test. The laboratory's result is compared with other (peer) laboratories performing the same test. EQA programs assign target values using a variety of methods statistical tools and performance assessment of 'pass' or 'fail' is made. EQA provider members of the international organization, external quality assurance in thrombosis and hemostasis, took part in a study to compare outcome of performance analysis using the same data set of laboratory results. Eleven EQA organizations using eight different analytical approaches participated. Data for a normal and prolonged activated partial thromboplastin time (aPTT) and a normal and reduced factor VIII (FVIII) from 218 laboratories were sent to the EQA providers who analyzed the data set using their method of evaluation for aPTT and FVIII, determining the performance for each laboratory record in the data set. Providers also summarized their statistical approach to assignment of target values and laboratory performance. Each laboratory record in the data set was graded pass/fail by all EQA providers for each of the four analytes. There was a lack of agreement of pass/fail grading among EQA programs. Discordance in the grading was 17.9 and 11% of normal and prolonged aPTT results, respectively, and 20.2 and 17.4% of normal and reduced FVIII results, respectively. All EQA programs in this study employed statistical methods compliant with the International Standardization Organization (ISO), ISO 13528, yet the evaluation of laboratory results for all four analytes showed remarkable grading discordance.

  18. Lack of grading agreement among international hemostasis external quality assessment programs

    Science.gov (United States)

    Olson, John D.; Jennings, Ian; Meijer, Piet; Bon, Chantal; Bonar, Roslyn; Favaloro, Emmanuel J.; Higgins, Russell A.; Keeney, Michael; Mammen, Joy; Marlar, Richard A.; Meley, Roland; Nair, Sukesh C.; Nichols, William L.; Raby, Anne; Reverter, Joan C.; Srivastava, Alok; Walker, Isobel

    2018-01-01

    Laboratory quality programs rely on internal quality control and external quality assessment (EQA). EQA programs provide unknown specimens for the laboratory to test. The laboratory's result is compared with other (peer) laboratories performing the same test. EQA programs assign target values using a variety of methods statistical tools and performance assessment of ‘pass’ or ‘fail’ is made. EQA provider members of the international organization, external quality assurance in thrombosis and hemostasis, took part in a study to compare outcome of performance analysis using the same data set of laboratory results. Eleven EQA organizations using eight different analytical approaches participated. Data for a normal and prolonged activated partial thromboplastin time (aPTT) and a normal and reduced factor VIII (FVIII) from 218 laboratories were sent to the EQA providers who analyzed the data set using their method of evaluation for aPTT and FVIII, determining the performance for each laboratory record in the data set. Providers also summarized their statistical approach to assignment of target values and laboratory performance. Each laboratory record in the data set was graded pass/fail by all EQA providers for each of the four analytes. There was a lack of agreement of pass/fail grading among EQA programs. Discordance in the grading was 17.9 and 11% of normal and prolonged aPTT results, respectively, and 20.2 and 17.4% of normal and reduced FVIII results, respectively. All EQA programs in this study employed statistical methods compliant with the International Standardization Organization (ISO), ISO 13528, yet the evaluation of laboratory results for all four analytes showed remarkable grading discordance. PMID:29232255

  19. Balancing Eggs

    Science.gov (United States)

    Mills, Allan

    2014-01-01

    Theory predicts that an egg-shaped body should rest in stable equilibrium when on its side, balance vertically in metastable equilibrium on its broad end and be completely unstable on its narrow end. A homogeneous solid egg made from wood, clay or plastic behaves in this way, but a real egg will not stand on either end. It is shown that this…

  20. Associations of Perceived Sibling and Parent-Child Relationship Quality with Internalizing and Externalizing Problems: Comparing Indian and Dutch Early Adolescents

    Science.gov (United States)

    Buist, Kirsten L.; Verhoeven, Marjolein; Hoksbergen, René; ter Laak, Jan; Watve, Sujala; Paranjpe, Analpa

    2017-01-01

    The aims of the present study were (a) to examine whether Dutch and Indian early adolescents differ concerning sibling and parent-child relationship quality and externalizing and internalizing problems, and (b) to compare the associations between sibling and parent-child relationship quality and externalizing and internalizing problems for Indian…

  1. GEOMETRICAL PARAMETERS OF EGGS IN BIRD SYSTEMATICS

    Directory of Open Access Journals (Sweden)

    I. S. Mityay

    2014-12-01

    Full Text Available Our ideas are based on the following assumptions. Egg as a standalone system is formed within another system, which is the body of the female. Both systems are implemented on the basis of a common genetic code. In this regard, for example, the dendrogram constructed by morphological criteria eggs should be approximately equal to those constructed by other molecular or morphological criteria adult birds. It should be noted that the dendrogram show only the degree of genetic similarity of taxa, therefore, the identity of materials depends on the number of analyzed criteria and their quality, ie, they should be the backbone. The greater the number of system-features will be included in the analysis and in one other case, the like are dendrogram. In other cases, we will have a fragmentary similarity, which is also very important when dealing with controversial issues. The main message of our research was to figure out the eligibility of usage the morphological characteristics of eggs as additional information in taxonomy and phylogeny of birds. Our studies show that the shape parameters of bird eggs show a stable attachment to certain types of birds and complex traits are species-specific. Dendrogram and diagrams built by the quantitative value of these signs, exhibit significant similarity with the dendrogram constructed by morphological, comparative anatomy, paleontology and molecular criteria for adult birds. This suggests the possibility of using morphological parameters eggs as additional information in dealing with taxonomy and phylogeny of birds. Keywords: oology, geometrical parameters of eggs, bird systematics

  2. Parental perceptions in egg allergy: does egg challenge make a difference?

    Science.gov (United States)

    Kemp, Andrew Stewart; Allen, Clare Wendy; Campbell, Dianne Elisabeth

    2009-11-01

    The aim of the study was to determine the effect of an oral egg challenge in egg sensitized children on parental perceptions relating to their child's allergy. A questionnaire was completed by parents for 167 children attending a tertiary paediatric clinic with egg sensitization. The questionnaires included 10 questions concerning parental perceptions of their child's egg allergy. Parental perceptions of those children who had not had an egg challenge (n = 83) were compared with those whose children had a positive (n = 27) and those with a negative (n = 57) egg challenge. A significant difference (p = parents in the CN group expected little or no future inconvenience for the child. The responses of parents whose child had undergone an egg challenge differed significantly (p = expectation of little or no future discomfort for the child and whether others treated the child differently. The performance of an egg challenge was associated with reduced adverse parental concerns. For 6/10 parameters, expectations concerning egg allergy in children who had been challenged were significantly better than those who had never been challenged irrespective of the challenge outcome. The greater certainty provided by the performance of a food challenge may be a positive outcome in both CP and CN children.

  3. Sanitation in the Shell Egg Processing Environment

    Science.gov (United States)

    In the past, most of the regulations regarding egg processing are concerned with quality rather than safety. Hazard Analysis and Critical Control Point (HACCP) will be required by retailers or by the federal government. GMPs (Good Manufacturing Practices) and SSOPs (Sanitation Standard Operating P...

  4. External quality assessment on detection of hepatitis C virus RNA in clinical laboratories of China.

    Science.gov (United States)

    Wang, Lu-nan; Zhang, Rui; Shen, Zi-yu; Chen, Wen-xiang; Li, Jin-ming

    2008-06-05

    As with many studies carried out in European countries, a quality assurance program has been established by the National Center for Clinical Laboratories in China (NCCL). The results showed that the external quality assessment significantly improves laboratory performance for quantitative evaluation of hepatitis C virus (HCV) RNA. Serum panels were delivered twice annually to the clinical laboratories which performed HCV RNA detection in China. Each panel made up of 5 coded samples. All laboratories were requested to carry out the detection within the required time period and report on testing results which contained qualitative and/or quantitative test findings, reagents used and relevant information about apparatus. All the positive samples were calibrated against the first International Standard for HCV RNA in a collaborative study and the range of comparison target value (TG) designated as +/- 0.5 log. The numbers of laboratories reporting on qualitative testing results for the first and second time external quality assessment were 168 and 167 in the year of 2003 and increased to 209 and 233 in 2007; the numbers of laboratories reporting on quantitative testing results were 134 and 147 in 2003 and rose to 340 and 339 in 2007. Deviation between the mean value for quantitative results at home in 2003 and the target value was above 0.5 log, which was comparatively high. By 2007, the target value was close to the national average except for the low concentrated specimens (10(3) IU/ml). The percentage of results within the range of GM +/- 0.5 log(10) varied from 8.2% to 93.5%. Some laboratories had some difficulties in the exact quantification of the lowest (3.00 log IU/ml) as well as of the highest viral levels (6.37 log IU/ml) values, very near to the limits of the dynamic range of the assays. The comparison of these results with the previous study confirms that a regular participation in external quality assessment (EQA) assures the achievement of a high

  5. Regional programme on quality control of radioimmunoassay: Development of human resources and external quality assessments

    International Nuclear Information System (INIS)

    Quiroga, S.; Torres, M.; Mendizabal, A.F.; Farinati, Z.; Galanternik, A.

    1986-01-01

    Since 1978 the authors have been concerned with helping to standardize radioimmunoassay (RIA) methodology in Argentina and other Latin American countries by: (1) developing human resources through courses on quality control of RIA and training of fellows, and (2) developing four external quality assessment (EQA) schemes to evaluate the performance of laboratories in determining several analytes by RIA. The number of collaborating laboratories increased between the first and fourth schemes. The average analytical performance achieved by the participants in each scheme was estimated by the average between-laboratory variation. Thyroxine, cortisol and tri-iodothyronine were measured the most accurately. Different problems were evident in the RIAs of thyrotrophin, luteinizing hormone, follicle stimulating hormone, prolactin, testosterone, progesterone, cortisol, immunoglobulin E and human growth hormone. RIA of oestradiol showed the worst accuracy. Analysis of the results showed an increasing interest in RIA quality control as it was found to improve the reliability of RIA. (author)

  6. 21 CFR 160.100 - Eggs.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Eggs. 160.100 Section 160.100 Food and Drugs FOOD... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.100 Eggs... identity for the food commonly known as eggs. ...

  7. [Analysis of the results of the SEIMC External Quality Control Program, 2007].

    Science.gov (United States)

    Guna Serrano, María del Remedio; Orta Mira, Nieves; Ovies, María; Gimeno Cardona, Concepción; Pérez, José L

    2008-11-01

    The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) includes controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology and molecular microbiology. This article presents the most important conclusions and lessons drawn from the 2007 controls. As a whole, the results obtained in 2007 confirm the excellent skill and good technical standards found in previous years. However, erroneous results can be obtained in any laboratory and in clinically relevant determinations. A few deviations were observed in some controls, calling for critical reflection. Once again, the results of this program highlighted the need to complement internal with external controls, such as those offered by the SEIMC program.

  8. Experimental Shifts in Intraclutch Egg Color Variation Do Not Affect Egg Rejection in a Host of a Non-Egg-Mimetic Avian Brood Parasite

    Science.gov (United States)

    Croston, Rebecca; Hauber, Mark E.

    2015-01-01

    Avian brood parasites lay their eggs in the nests of other birds, and impose the costs associated with rearing parasitic young onto these hosts. Many hosts of brood parasites defend against parasitism by removing foreign eggs from the nest. In systems where parasitic eggs mimic host eggs in coloration and patterning, extensive intraclutch variation in egg appearances may impair the host’s ability to recognize and reject parasitic eggs, but experimental investigation of this effect has produced conflicting results. The cognitive mechanism by which hosts recognize parasitic eggs may vary across brood parasite hosts, and this may explain variation in experimental outcome across studies investigating egg rejection in hosts of egg-mimicking brood parasites. In contrast, for hosts of non-egg-mimetic parasites, intraclutch egg color variation is not predicted to co-vary with foreign egg rejection, irrespective of cognitive mechanism. Here we tested for effects of intraclutch egg color variation in a host of nonmimetic brood parasite by manipulating egg color in American robins (Turdus migratorius), hosts of brown-headed cowbirds (Molothrus ater). We recorded robins’ behavioral responses to simulated cowbird parasitism in nests where color variation was artificially enhanced or reduced. We also quantified egg color variation within and between unmanipulated robin clutches as perceived by robins themselves using spectrophotometric measures and avian visual modeling. In unmanipulated nests, egg color varied more between than within robin clutches. As predicted, however, manipulation of color variation did not affect rejection rates. Overall, our results best support the scenario wherein egg rejection is the outcome of selective pressure by a nonmimetic brood parasite, because robins are efficient rejecters of foreign eggs, irrespective of the color variation within their own clutch. PMID:25831051

  9. Experimental shifts in intraclutch egg color variation do not affect egg rejection in a host of a non-egg-mimetic avian brood parasite.

    Directory of Open Access Journals (Sweden)

    Rebecca Croston

    Full Text Available Avian brood parasites lay their eggs in the nests of other birds, and impose the costs associated with rearing parasitic young onto these hosts. Many hosts of brood parasites defend against parasitism by removing foreign eggs from the nest. In systems where parasitic eggs mimic host eggs in coloration and patterning, extensive intraclutch variation in egg appearances may impair the host's ability to recognize and reject parasitic eggs, but experimental investigation of this effect has produced conflicting results. The cognitive mechanism by which hosts recognize parasitic eggs may vary across brood parasite hosts, and this may explain variation in experimental outcome across studies investigating egg rejection in hosts of egg-mimicking brood parasites. In contrast, for hosts of non-egg-mimetic parasites, intraclutch egg color variation is not predicted to co-vary with foreign egg rejection, irrespective of cognitive mechanism. Here we tested for effects of intraclutch egg color variation in a host of nonmimetic brood parasite by manipulating egg color in American robins (Turdus migratorius, hosts of brown-headed cowbirds (Molothrus ater. We recorded robins' behavioral responses to simulated cowbird parasitism in nests where color variation was artificially enhanced or reduced. We also quantified egg color variation within and between unmanipulated robin clutches as perceived by robins themselves using spectrophotometric measures and avian visual modeling. In unmanipulated nests, egg color varied more between than within robin clutches. As predicted, however, manipulation of color variation did not affect rejection rates. Overall, our results best support the scenario wherein egg rejection is the outcome of selective pressure by a nonmimetic brood parasite, because robins are efficient rejecters of foreign eggs, irrespective of the color variation within their own clutch.

  10. The development of regional markets of eggs production in Russia

    Directory of Open Access Journals (Sweden)

    Natal'ya Anatol'evna Alekseeva

    2011-12-01

    Full Text Available The current state of regional markets development and the production of eggs and egg products for a long period — from 1990 to 2009 — is reviewed in this paper. The main research method is the method of statistical groupings. The variable amount of egg production was chosen as the grouping characteristic, since this figure fits well into research goals and has the property of the prevalence in the aggregate / total. To analyze the trends of the Russian regional markets involved into egg production, official statistics on consumer prices was used as well as producer prices, the volume of egg production per capita based on food import and export products. This allowed to group regions for comparison with average values of indicators carried out by different criteria, and to draw conclusions about trends in the development of regional markets. The most relevant factors influencing the development of egg production, among which the factor of vertical integration of production was especially noticed: building own plants and feed mills, joining the land in order to ensure the needs for higher quality raw materials and other activities were identified.

  11. Effect of feeding low-fiber fraction of air-classified sunflower (Helianthus annus L.) meal on laying hen productive performance and egg yolk cholesterol.

    Science.gov (United States)

    Laudadio, V; Ceci, E; Lastella, N M B; Tufarelli, V

    2014-11-01

    The present study was designed to determine the effect on laying performance and egg quality resulting from total substitution of soybean meal (SBM) with low-fiber sunflower meal (SFM; Helianthus annus L.) meal in diet of hens. ISA Brown layers, 28 wk of age, were randomly allocated to 2 dietary treatments and fed for 10 wk. The hens were kept in a free-range environment and fed 2 wheat middling-based diets consisting of a control diet, which contained SBM (153 g/kg of diet), and a test diet containing low-fiber SFM (160 g/kg of diet) as the main protein source. Each dietary treatment was replicated 4 times. Low-fiber SFM was obtained by a combination of sieving and air classification processes. Feed consumption was recorded daily and egg production was calculated on a hen-day basis; eggs from each group were collected weekly to evaluate egg components and quality. The total substitution of SBM with low-fiber SFM had no adverse effect on growth performance of laying hens. Egg production and none of egg quality traits examined were influenced by dietary treatment, except for yolk color (P eggs (P egg yolk total cholesterol and low-density lipoprotein cholesterol concentrations (P egg quality and to develop low-cholesterol eggs. ©2014 Poultry Science Association Inc.

  12. Egg yolk plasma can replace egg yolk in stallion freezing extenders.

    Science.gov (United States)

    Pillet, E; Duchamp, G; Batellier, F; Beaumal, V; Anton, M; Desherces, S; Schmitt, E; Magistrini, M

    2011-01-01

    Hen egg yolk is normally used as a cryoprotective agent in semen freezing extenders, but its use has sanitary and practical disadvantages. Moreover the protection afforded by egg yolk has not yet been completely elucidated. The objective of this study was to compare the egg yolk plasma fraction to whole egg yolk in stallion freezing extender. Plasma contains mainly Low Density Lipoproteins (LDL), which are widely presumed to be the cryoprotective agent in egg yolk. Plasma can be produced on an industrial scale, sterilised by gamma-irradiation and incorporated in a ready-to-use extender (our ultimate objective). Plasma samples were subjected to different doses of gamma-irradiation (3, 5, 10 kGy) without dramatic chemical changes that may affect their cryoprotective properties. Stallion semen was frozen with whole egg yolk as a control and with sterilised egg yolk plasma. A fertility trial was conducted on a total of 70 mares' cycles. Fertility per cycle was 60% after insemination of semen frozen in our control extender containing egg yolk (EY), compared to 69% for the extender containing sterilised egg yolk plasma (EYP) (P > 0.05). Post-thaw motility and membrane integrity of spermatozoa were also analysed. Motility parameters were not significantly different between extenders except for the variable VAP (for EY versus EYP, VAP: 63 μm.s(-1) versus 59 μm.s(-1), a, b: P 0.05). Membrane integrity was better preserved in EY than in EYP but the difference between extenders was small (P < 0.05). Our results demonstrated that sterilised egg yolk plasma has the potential to replace egg yolk in stallion freezing extender. This experiment led to the development of a ready-to-use extender called INRA-Freeze(®) (IMV-Technologies, France). Copyright © 2011 Elsevier Inc. All rights reserved.

  13. Pilot Experience with an External Quality Assurance Scheme for Acylcarnitines in Plasma/Serum

    NARCIS (Netherlands)

    Sala, P Ruiz; Ruijter, G; Acquaviva, C; Chabli, A; de Sain-van der Velden, M G M; Garcia-Villoria, J; Heiner-Fokkema, M R; Jeannesson-Thivisol, E; Leckstrom, K; Franzson, L; Lynes, G; Olesen, J; Onkenhout, W; Petrou, P; Drousiotou, A; Ribes, A; Vianey-Saban, C; Merinero, B

    2016-01-01

    The analysis of acylcarnitines (AC) in plasma/serum is established as a useful test for the biochemical diagnosis and the monitoring of treatment of organic acidurias and fatty acid oxidation defects. External quality assurance (EQA) for qualitative and quantitative AC is offered by ERNDIM and CDC

  14. Fatty Acid Composition of Egg Yolk from Chickens Fed a Diet including Marigold (Tagetes erecta L.

    Directory of Open Access Journals (Sweden)

    A. Altuntaş

    2014-01-01

    Full Text Available The objective of this study was to determine the effects of diet supplemented with marigold on egg yolk fatty acid composition and egg quality parameters. Sixty hens were assigned into three groups and fed diets supplemented with 0 (control, 10 g kg−1, or 20 g kg−1 marigold for 42 days. Eggs collected at the 6th week of the study were analyzed for fatty acid analysis. Laying performance, egg quality parameters, and feed intake were also evaluated. Yolk color scores in the group fed the 20 g kg−1 marigold-supplemented diet were found greater than control (10.77 versus 9.77. Inclusion of 20 g kg−1 marigold in diet influenced egg weights adversely compared to the control. Diet supplemented with 10 g kg−1 or 20 g kg−1 marigold increased the levels of C16:0 and C18:0 and decreased levels of C16:1 (n-7 and C18:1 (n-9 in the egg yolk. Also, diet including marigold increased total saturated fatty acids (SFA and decreased monounsaturated fatty acids (MUFA in the egg yolk.

  15. Bacterial contamination of eggs and behaviour of poultry flocks in the free range environment.

    Science.gov (United States)

    Moyle, Talia; Drake, Kelly; Gole, Vaibhav; Chousalkar, Kapil; Hazel, Susan

    2016-12-01

    The free range production system is becoming more common in Australia and is expected to increase. Free range hens are exposed to more stressors in comparison to hens from barn and cage systems and it is suggested that stress can increase bacterial shedding on eggs. The aims of this study were to examine the level of total bacteria and Enterobacteriaceae populations, as well as the presence of Salmonella and Campylobacter, in eggs collected from two free range flocks on two different farms and to conduct longitudinal observations of the behaviour and welfare of hens in the free range production system. Hen age (weeks) was shown to have a significant effect (increase) on the level of total bacteria on the egg shell surface and in shell pores, as well as having an effect on feather condition score. As the hens aged, the frequency of external visual egg characteristics increased, as did feather condition score (where feather condition was poorer). These observations indicate areas which should be investigated further to improve the food safety of eggs and optimise the welfare of free range hens. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

  16. Resuspension and settling of helminth eggs in water: Interactions with cohesive sediments.

    Science.gov (United States)

    Sengupta, Mita E; Andersen, Thorbjørn J; Dalsgaard, Anders; Olsen, Annette; Thamsborg, Stig M

    2012-08-01

    Helminth parasite eggs in low quality water represent main food safety and health hazards and are therefore important indicators used to determine whether such water can be used for irrigation. Through sedimentation helminth eggs accumulate in the sediment, however resuspension of deposited helminth eggs will lead to increased concentration of suspended eggs in the water. Our study aimed to determine the erodibility (erosion rate and erosion threshold) and settling velocity of Ascaris and Trichuris eggs as well as cohesive sediment at different time points after incorporation into the sediment. Cohesive sediment collected from a freshwater stream was used to prepare a sediment bed onto which helminth eggs were allowed to settle. The erodibility of both sediment and helminth eggs was found to decrease over time indicating that the eggs were incorporated into the surface material of the bed and that this material was stabilized through time. This interaction between eggs and bulk sediment was further manifested in an increased settling velocity of suspended eggs when sediment was present in the suspension as compared to a situation with settling in clean water. The incorporation into the sediment bed and the aggregation with sediment particles decrease the mobility of both helminth egg types. Our findings document that helminth eggs should not be viewed as single entities in water systems when modelling the distribution of eggs since both erodibility and settling velocity of eggs are determined by mobility of the sediment present in the water stream. Recalculation of the erosion threshold for helminth eggs and sediment showed that even at relatively low current velocities i.e. 0.07-0.12ms(-1) newly deposited eggs will be mobile in open irrigation channels. These environmental factors affecting resuspension must be taken into account when developing models for sedimentation of helminth eggs in different water systems. Copyright © 2012 Elsevier Ltd. All rights

  17. Comparison of yolk fatty acid content, blood and egg cholesterol of ...

    African Journals Online (AJOL)

    Administrator

    2011-09-07

    POO) and Kilka fish oil. (KFO) on yolk fatty acid content .... Egg quality parameters such as; haugh unit score, yolk color index (as measured by ..... oil) diet on performance, carcass quality and fat status in broilers. Afr. J. Biotechnol.

  18. Eggs and hatchlings variations in desert locusts: phase related characteristics and starvation tolerance

    Directory of Open Access Journals (Sweden)

    Koutaro Ould Maeno

    2013-12-01

    Full Text Available Locusts are grasshopper species that express phase polyphenism: modifying their behavior, morphology, coloration, life history and physiology in response to crowding. Desert locusts, Schistocerca gregaria, epigenetically modify progeny quality and quantity in response to crowding. Gregarious (crowded females produce larger but fewer progeny than do solitarious (isolated ones. The variability of progeny quality within single egg pod and the reasons why gregarious progeny have a better survival than solitarious ones remains unclear. This study investigated 1 the effects of rearing density on the variation in egg size within single egg pods 2 the starvation tolerance of hatchlings from mothers with different phases and 3 the physiological differences in hatchling energy reserve. Isolated females produced smaller but more eggs than did crowded ones. The variation in egg size within egg pods was greater in the latter than in the former. A negative relationship between egg size and number of eggs per egg pod was observed for both groups. Under starvation conditions, gregarious hatchlings survived significantly longer than solitarious ones. Among the solitarious hatchlings, the survival time was longer as hatchling body size increased. However, small individuals survived as long as large ones among the gregarious hatchlings. The percentage of water content per fresh body weight was almost equal between the two phases, before and after starvation. In contrast, the percentage of lipid content per dry body weight was significantly higher in gregarious hatchlings than in solitarious ones before starvation, but became almost equal after starvation. These results demonstrated that female locusts not only trade-off to modify their progeny size and number, but also vary progenies’ energy reserves. We hypothesized that gregarious females enhance their fitness by producing progeny differently adapted to high environmental variability and particularly to

  19. Have eggs. Will travel

    DEFF Research Database (Denmark)

    Kroløkke, Charlotte

    2015-01-01

    Feminist scholars have critically questioned the practices and ethics of reproductive mobility. While the reproductive mobility of fertility patients has been foregrounded, little is known of egg donor mobility including the experiences of travelling internationally to donate eggs. Based on written...... stories and photographic material provided by forty-two egg donors, this article uses feminist cluster analysis and the concept of eggpreneurship to illustrate how global egg donors negotiate reproductive agency and choice when they travel internationally to donate their eggs. In their stories, global egg...

  20. Production performance, use of nest box, and external appearance of two strains of laying hens kept in conventional and enriched cages.

    Science.gov (United States)

    Onbaşılar, E E; Ünal, N; Erdem, E; Kocakaya, A; Yaranoğlu, B

    2015-04-01

    The aim of this study was to investigate the differences in production performance, use of nest box, and external appearance of 2 strains of laying hens kept in conventional and enriched cages. Lohmann Brown Classic (LB, n=532) and Lohmann LSL Classic (LW, n=532) hens were housed from 16 to 73 wk in either conventional cages or enriched cages. Enriched cages had a nesting area, scratch pad, perch, and nail shortener. Body weight (BW), hen-day egg production, egg weight, feed intake, feed conversion ratio (FCR), cracked and dirty eggs, use of nest box for lay, and external appearance were determined. Laying period influenced the hen-day egg production, egg weight, feed intake, and feed conversion ratio. Cage type affected the hen-day egg production and feed conversion ratio, while strain affected the egg weight, feed intake, and feed conversion ratio. Laying period×cage type and laying period×strain interactions affected egg production, egg weight, and feed conversion ratio. Both strains preferred to lay in the nest box. Percentages of cracked and dirty eggs of LW hens in enriched cages were higher than that in conventional cages. Most of the dirty eggs laid by both strains were found outside of the nest box. The LW hens laid more dirty eggs than the LB hens. Cage type and cage type×strain interaction were important for total feather score. Final claw length was affected by cage type, strain, and cage type×strain interaction. This study suggests that cage type, strain, and also cage type×strain and period×strain interactions should be considered when alternative housing systems are used. © 2015 Poultry Science Association Inc.

  1. Effect of Dietary L-ascorbic Acid (L-AA on Production Performance, Egg Quality Traits and Fertility in Japanese Quail ( at Low Ambient Temperature

    Directory of Open Access Journals (Sweden)

    N. Shit

    2012-07-01

    Full Text Available Environmental stress boosts the levels of stress hormones and accelerates energy expenditure which subsequently imbalance the body’s homeostasis. L-ascorbic acid (L-AA has been recognized to mitigate the negative impact of environmental stress on production performances in birds. The present investigation was carried out to elucidate the effect of different dietary levels of L-AA on production performance, egg quality traits and fertility in Japanese quail at low ambient temperature. Sixty matured females (15 wks were equally divided into three groups (20/group based on the different dietary levels of L-AA (0, 250 and 500 ppm and coupled with an equal number of males (1:1 obtained from the same hatch. They were managed in uniform husbandry conditions without restriction of feed and water at 14 h photo-schedule. Except for feed efficiency, body weight change, feed consumption and hen-day egg production were recorded highest in 500 ppm L-AA supplemented groups. Among the all egg quality traits studied, only specific gravity, shell weight and thickness differed significantly (p<0.05 in the present study. Fertility was improved significantly (p<0.01 to a dose dependent manner of L-AA. The findings of the present study concluded that dietary L-AA can be a caring management practice at least in part to alleviate the adverse effect of cold induced stress on production performance in Japanese quail.

  2. Effect of group size on performance and egg quality of laying hens during 20 to 36 weeks of age

    Directory of Open Access Journals (Sweden)

    Fulvia Bovera

    2014-03-01

    Full Text Available The aim of this study was to improve knowledge on the effect of group size on productive performance and egg quality of hens raised in furnished cages equally designed. A total of 520, 15-week-old Lohmann Brown laying hens were divided into 2 groups to have a similar initial body weight (average 1392±16.3 g. The cages of S25 group (240 L x 78 W x 50 H cm, 749 cm2/hen hosted a total of 200 hens, while those of S40 group (462 L x 65 W x 50 H, 751 cm2/hen included 320 birds. Experimental data were recorded after an adaptation period of 5 weeks (20 to 36 weeks of age. Hens were submitted to 15 h of light/d. The average temperature inside the building was 24.6±2.5°C over the entire experimental period with higher values at 24, 26, 28 and 30 weeks of age. The relative humidity recorded inside the building was 55% at week 20 and 60% all through the experimental period. Hens raised from S40 group had lower percentage of egg production (84.91 vs 88.90%, P<0.01 and higher feed conversion ratio (2.70 vs 2.25, P<0.0001 than S25 group. The percentage of eggs laid out of the nest was higher in S25 than S40 group (0.26 vs 0.19%, P<0.01. As expected, the week of age affected almost all the parameters (feed intake, body weight, laying percentage, egg weight, yolk, shell and albumen indexes, shell thickness, Haugh unit. However, the effect of group size was particularly evident during the hot period.

  3. Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster

    DEFF Research Database (Denmark)

    Schou, Mads Fristrup

    2013-01-01

    When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column...... and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness...... and to obtain a representative collection of genotypes, the method presented here is strongly recommended when collecting eggs from Drosophila....

  4. 21 CFR 160.115 - Liquid eggs.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Liquid eggs. 160.115 Section 160.115 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.115 Liquid eggs. (a) Liquid eggs, mixed eggs, liquid whole eggs, mixed whole eggs are eggs of the domestic...

  5. The effect of selected feed additives on the shell qualitative parameters of table eggs

    Directory of Open Access Journals (Sweden)

    Henrieta Arpášová

    2015-05-01

    Full Text Available Herbs, spices and their extracts (botanicals have a wide range of activities. May have a beneficial effect on the gastrointestinal microflora of animals, performance and quality of animal products. In this experiment the effects of supplementation of the diet for laying hens with different doses of thyme or oregano essential oil addition on egg shell quality parameters were studied. Hens of laying hybrid Hy-Line Brown (n=50 were randomly divided into 5 groups (n=10 and fed for 20 weeks with diets with thyme or oregano essential oil. supplemented. In the control group hens received feed mixture with no additions. The diets in the first and  second experimental groups were supplemented with 0.5 ml/kg or 1.0 ml/kg thyme essential oil. The diets in the third and fourth experimental groups were supplemented with 0.5 ml/kg or 1.0 ml/kg oregano essential oil.  The egg shell weight (g, specific egg shell weight (g/cm3, percentage of egg shell (%, egg shell strength (N/cm2 and egg shell thickness (mm were evaluated. The egg shell weight for the whole period was in the order of the groups 5.70±0.52; 5.65±0.44; 5.54±0.42; 5.62±0.38 and 5.49±0.48 g±S.D (P>0.05. Egg shell strength during the reporting period was in order of the groups: 27.81±6.00; 27.63±6.43; 27.17±6.36; 27.76±6.27 and 28.41±6.36 (N/cm2±S.D. Similarly, in the egg shell specific weight (g/cm3, egg shell percentage ratio (% and egg shell thickness (mm were observed statistically non-significant differences compared to the control group (P>0.05. The results suggest that the qualitative parameters of egg shell were not significantly influenced with thyme or oregano oil addition (P>0.05.

  6. GEOMETRICAL PARAMETERS OF EGGS IN BIRD SYSTEMATICS

    Directory of Open Access Journals (Sweden)

    Mityay I.S.

    2014-12-01

    Full Text Available Our ideas are based on the following assumptions. Egg as a standalone system is formed within another system, which is the body of the female. Both systems are implemented on the basis of a common genetic code. In this regard, for example, the dendrogram constructed by morphological criteria eggs should be approximately equal to those constructed by other molecular or morphological criteria adult birds. It should be noted that the dendrogram show only the degree of genetic similarity of taxa, therefore, the identity of materials depends on the number of analyzed criteria and their quality, ie, they should be the backbone. The greater the number of system-features will be included in the analysis and in one other case, the like are dendrogram. In other cases, we will have a fragmentary similarity, which is also very important when dealing with controversial issues. The main message of our research was to figure out the eligibility of usage the morphological characteristics of eggs as additional information in taxonomy and phylogeny of birds. Our studies show that the shape parameters of bird eggs show a stable attachment to certain types of birds and complex traits are species-specific. Dendrogram and diagrams built by the quantitative value of these signs, exhibit significant similarity with the dendrogram constructed by morphological, comparative anatomy, paleontology and molecular criteria for adult birds. This suggests the possibility of using morphological parameters eggs as additional information in dealing with taxonomy and phylogeny of birds.

  7. Efficacy of various protein-based coating on enhancing the shelf life of fresh eggs during storage.

    Science.gov (United States)

    Caner, Cengiz; Yüceer, Muhammed

    2015-07-01

    The effectiveness of various coatings (whey protein isolate [WPI], whey protein concentrate [WPC], zein, and shellac) on functional properties, interior quality, and eggshell breaking strength of fresh eggs were evaluated during storage at 24 °: C for 6 weeks. Coatings and storage time had significant effects on Haugh unit, yolk index, albumen pH, dry matter (DMA), relative whipping capacity (RWC), and albumen viscosity. Uncoated eggs had higher albumen pH (9.56) and weight loss, and lower albumen viscosity (5.73), Haugh unit (HU), and yolk index (YI) during storage. Among the coated eggs, the shellac and zein coated eggs had the highest value of albumen viscosity (27.26 to 26.90), HU (74.10 to 73.61), and YI (44.84 to 44.63) after storage. Shellac (1.44%) was more effective in preventing weight loss than WPC (4.59%), WPI (4.60%), and zein (2.13%) coatings. Uncoated eggs had the higest value (6.71%) of weight lost. All coatings increased shell strength (5.18 to 5.73 for top and 3.58 to 4.71 for bottom) significantly (P eggs (4.70 for top and 3.15 for bottom). The functional properties such as albumen DMA (14.50 to 16.66 and 18.97 for uncoated) and albumen RWC (841 to 891 and 475 for uncoated) of fresh eggs can be preserved during storage when they are coated. The shellac and zein coatings were more effective for maintaining the internal quality of fresh eggs during storage. Fourier transform near infrared (FT-NIR) in the 800 to 2500 nm reflection spectra were used to quantify the contents of the fresh eggs at the end of storage. Eggs coated with shellac or zein displayed a higher absorbance at 970 and 1,197 nm respectively (OH vibration of water) compared with those coated with WPI or WPC and the uncoated group at the end of storage. The coatings improved functional properties and also shell strength and could be a viable alternative technology for maintaining the internal quality of eggs during long-term storage. This study highlights the promising use of

  8. The Role of Baked Egg and Milk in the Diets of Allergic Children.

    Science.gov (United States)

    Robinson, Melissa L; Lanser, Bruce J

    2018-02-01

    Baked egg and baked milk are tolerated by most children who are allergic to hen's egg and cow's milk. Incorporating baked goods into the diets of allergic children may help them outgrow their primary allergy more quickly, with changes observed akin to immunotherapy. Benefits may also include increased quality of life and improved nutritional status. The search for a reliable biomarker to predict tolerance to baked goods is ongoing. Most children with a milk or egg allergy who are not previously tolerating egg or milk in baked goods should be offered an observed oral food challenge. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor

    Science.gov (United States)

    Chai, Yating; Li, Suiqiong; Horikawa, Shin; Shen, Wen; Park, Mi-Kyung; Vodyanoy, Vitaly J.; Chin, Bryan A.

    2011-06-01

    This paper presents the direct detection of Salmonella typhimurium on shell eggs using a phage-based magnetoelastic (ME) biosensor. The ME biosensor consists of a ME resonator as the sensor platform and E2 phage as the biorecognition element that is genetically engineered to specifically bind with Salmonella typhimurium. The ME biosensor, which is a wireless sensor, vibrates with a characteristic resonant frequency under an externally applied magnetic field. Multiple sensors can easily be remotely monitored. Multiple measurement and control sensors were placed on the shell eggs contaminated by Salmonella typhimurium solutions with different known concentrations. The resonant frequency of sensors before and after the exposure to the spiked shell eggs was measured. The frequency shift of the measurement sensors was significantly different than the control sensors indicating Salmonella contamination. Scanning electron microscopy was used to confirm binding of Salmonella to the sensor surface and the resulting frequency shift results.

  10. External quality assurance programme for enzymatic analysis of lysosomal storage diseases : A pilot study

    NARCIS (Netherlands)

    Ruijter, G.J.G.; Boer, M.; Weykamp, C. W.; de Vries, R.; van den Berg, I.; Janssens-Puister, J.; Niezen-Koning, K.; Wevers, R. A.; Poorthuis, B. J. H. M.; van Diggelen, O. P.

    2005-01-01

    Inborn errors of metabolism are rare and laboratories performing diagnostic tests in this field must participate in external quality assurance (EQA) schemes to demonstrate their competence and also to maintain sufficient experience with patient material. EQA schemes for metabolite analyses are

  11. Use of irradiation for decontamination of chicken and spray-dried whole egg powder from Salmonellae

    International Nuclear Information System (INIS)

    Koehler, B.; Huebner, H.

    1989-01-01

    The effect of 60 Co gamma irradiation was investigated on the concentration of Salmonella (S.) typhimurium in artificially contaminated chicken carcasses and on the number of S. tennessee and S. agona in artificially contaminated spary-dried whole egg powder and liquid whole egg. Irradiation of carcasses and of liquid whole egg was carried out at deep frozen conditions between -10 0 C and -18 0 C and whole egg powder at 15-20 0 C. The irradiation doses used where between 0.05 and 8.0 kGy. The D 10 -value of S. typhimurium in broiler chicks ranged between 0.57 and 0.74 and of S. agona and S. tennessee, resp., amounted to 0.95 and 1.07 in spray dried whole egg powder and 0.47 and 0.53, resp., in whole egg content. Irradiation caused drastic reduction of physiological microflora in chicken carcasses. By radiation treatment using a dose of 4.0 kGy, germ count reductions by 3-5 decimal powers were achieved. Resistance of microbes increased in following succession: Pseudomonas spp., Salmonella spp., other Enterobacteriaceae spp., Micrococcus spp., Flavobacteriaceae spp., yeasts. Bacillus spp., Streptomyces spp., fungi. Results of microbiological and sensorial examinations allow following conclusions: (1) irradiation is highly effective against Salmonella. (2) Irradiation dose of 4.0 kGy is able to kill about 1 million Salmonellae per chicken. This guarantees safe elimination of naturally occurring Salmonellae in broiler chicken carcasses. (3) Irradiation of chicken carcasses up to 4.0 kGy causes no deterioration of quality, however storage longer than 4 months deteriorates sensorial qualities more than of unirradiated carcasses. (4) Whole egg powder tolerates a maximal irradiation dose of 2.0 kGy without deterioration of sensorial food quality. (5) Irradiation dose of 1.0 kGy eliminates about 100 to 1000 Salmonellae per kg whole egg powder, if the egg powder is stored 3 to 5 weeks after irradiation. (author)

  12. External Quality Arrangements for the Review of Modern Apprenticeship Off-the-Job Training

    Science.gov (United States)

    Education Scotland, 2015

    2015-01-01

    This publication provides a quality framework to provide a structure for external review of Modern Apprenticeship off-the-job training.? The indicators in this framework are arranged under three key principles, addressing five questions which Education Scotland is adopting for evaluation purposes. These are underpinned by a wider principle on the…

  13. HPLC Quantification of astaxanthin and canthaxanthin in Salmonidae eggs.

    Science.gov (United States)

    Tzanova, Milena; Argirova, Mariana; Atanasov, Vasil

    2017-04-01

    Astaxanthin and canthaxanthin are naturally occurring antioxidants referred to as xanthophylls. They are used as food additives in fish farms to improve the organoleptic qualities of salmonid products and to prevent reproductive diseases. This study reports the development and single-laboratory validation of a rapid method for quantification of astaxanthin and canthaxanthin in eggs of rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis М.). An advantage of the proposed method is the perfect combination of selective extraction of the xanthophylls and analysis of the extract by high-performance liquid chromatography and photodiode array detection. The method validation was carried out in terms of linearity, accuracy, precision, recovery and limits of detection and quantification. The method was applied for simultaneous quantification of the two xanthophylls in eggs of rainbow trout and brook trout after their selective extraction. The results show that astaxanthin accumulations in salmonid fish eggs are larger than those of canthaxanthin. As the levels of these two xanthophylls affect fish fertility, this method can be used to improve the nutritional quality and to minimize the occurrence of the M74 syndrome in fish populations. Copyright © 2016 John Wiley & Sons, Ltd.

  14. External Validation of the Acoustic Voice Quality Index Version 03.01 With Extended Representativity.

    Science.gov (United States)

    Barsties, Ben; Maryn, Youri

    2016-07-01

    The Acoustic Voice Quality Index (AVQI) is an objective method to quantify the severity of overall voice quality in concatenated continuous speech and sustained phonation segments. Recently, AVQI was successfully modified to be more representative and ecologically valid because the internal consistency of AVQI was balanced out through equal proportion of the 2 speech types. The present investigation aims to explore its external validation in a large data set. An expert panel of 12 speech-language therapists rated the voice quality of 1058 concatenated voice samples varying from normophonia to severe dysphonia. The Spearman rank-order correlation coefficients (r) were used to measure concurrent validity. The AVQI's diagnostic accuracy was evaluated with several estimates of its receiver operating characteristics (ROC). Finally, 8 of the 12 experts were chosen because of reliability criteria. A strong correlation was identified between AVQI and auditoryperceptual rating (r = 0.815, P = .000). It indicated that 66.4% of the auditory-perceptual rating's variation was explained by AVQI. Additionally, the ROC results showed again the best diagnostic outcome at a threshold of AVQI = 2.43. This study highlights external validation and diagnostic precision of the AVQI version 03.01 as a robust and ecologically valid measurement to objectify voice quality. © The Author(s) 2016.

  15. External bone marrow cytological examination quality assurance (EQAhem)--summary after 6 years in Poland.

    Science.gov (United States)

    Lewandowski, Krzysztof; Kurpierz, Katarzyna; Sledzinska, Anna

    2015-10-01

    Bone marrow macroscopic examination remains one of the most difficult and subjective laboratory assessments in hematology. Only a few external quality assurance programs in the field are present worldwide. We have developed an external quality assurance program EQAhem that allows assessment of the whole process of bone marrow examination. The program participants assess blood and bone marrow smears from the patient, identify selected cells from photographs provided to them, and interpret the microscopic results. In this article, the results of the EQAhem program in Poland from 6 years are summarized. During this time, 62 labs were assessed in total, and positive results were achieved by 89.25 % labs, taking into account all tests. Correct responses with respect to the percentage of cell count were provided by ca. 77.5 % labs. Slightly worse results were obtained when megakaryocyte count and cell identification from photographs were tested. The worst results were obtained in case of dysplasia assessment and clinical interpretation of microscopic examination (54.1 and 58.6 % correct responses, respectively). EQAhem delivers precise information about the quality of bone marrow examinations performed in Poland and has a substantial educational value. We believe that after 6 years, EQAhem has significantly improved the quality of bone marrow microscopic examinations performed in Poland.

  16. Egg origin determination efforts

    International Nuclear Information System (INIS)

    Horvath, A.; Futo, I.; Vodila, G.; Palcsu, L.

    2012-01-01

    Complete text of publication follows. As a co-operation with the Poultry Product Board, egg and drinking water samples were received in order to investigate whether the country of origin of the egg can be determined based on its stable isotope composition with the aim of market protection of the Hungarian eggs against the mislabelled foreign ones. The scientific background is that drinking water of egg laying hens is assumed to reflect the composition of regional precipitation, and it is also an input data in the process of egg formation. In the first sampling, altogether 23 sets of egg and drinking water samples were received from different production sites covering the whole area of Hungary. The egg white samples were vacuum distilled and frozen out by liquid nitrogen at -196 deg C. The process was monitored by two vacuum gauges. Water frozen out together with the drinking water samples was measured were measured by a Thermo Finnigan Delta PLUS XP isotope ratio mass spectrometer using a GasBench II peripheral unit equipped with a GC-autosampler. As a second issue, additionally, elemental composition of egg shells were also performed for series of Hungarian, Czech and Polish egg samples by energy dispersive X-ray fluorescence. The drinking waters fit well to the Global Meteoric Water Line indicating their precipitation origin. It was experienced that the water in egg white gets enriched compared to the drinking water (Δ 18 O = -4.9 ± 1.0 per thousand and Δ D = -21.8 ± 6.4 per thousand), however, this shift is independent of the type of the hens, since the mean shifts in the eggs of Tetra and Hy-line hens are similar within error bar. For more depleted drinking water, the shift of the egg white was higher than for more enriched ones. This can be due to the contribution of the nutriment isotopic composition. The water isotope composition of the Hungarian eggs investigated was δ 18 O = -4.8 - -7.3 per thousand and δD = -46.0 - -70.7 per thousand, therefore egg

  17. In p nduce protoc d spaw cols of wning hormo of Liza ones w egg a ...

    African Journals Online (AJOL)

    SAM

    environmental conditions and resistance to disease and stresses. Thin-lipped ... ship between egg quality and fatty acid content of eggs. Successful fertilization ...... mullet (Mugil cephalus L.) with fractional salmon pituitary extract. J. Fish Biol.

  18. Effect of combination of chitosan coating and irradiation on physicochemical and functional properties of chicken egg during room-temperature storage

    International Nuclear Information System (INIS)

    Liu Xianxe; Jang, Aera; Kim, Dong Hun; Lee, Bong Duk; Lee, Mooha; Jo, Cheorun

    2009-01-01

    The effect of combination of chitosan coating and irradiation on quality and storage stability of shell egg was investigated. Salmonella typhimurium inoculated on eggshell was not detected by irradiation of 2.0 kGy at day 0 and/or chitosan coating (1%, pH 5.0) after 3 days of storage. One-day-old fresh chicken egg was chitosan coated and irradiated at 0, 0.5, 1.0, 1.5 and 2.0 kGy by gamma ray. The egg samples were stored at room temperature for 14 days and the effects of the combination treatment on internal physicochemical and functional properties were investigated. The Haugh unit of egg was decreased by irradiation even at 0.5 kGy. Irradiation increased the lipid oxidation in egg yolk at 2 kGy but the egg with chitosan coating reduced the level of lipid oxidation. Irradiation increased the foaming ability of egg white and decreased viscosity of egg yolk and white. Results suggested that combination of irradiation and chitosan coating can improve safety of shell egg but irradiation treatment may reduce the egg quality for direct consumption. However, an improved functional property for further processing and efficient separation of egg white and yolk can be expected for egg processing industry using irradiation.

  19. Radionuclides in Canada goose eggs

    International Nuclear Information System (INIS)

    Rickard, W.H.; Sweany, H.A.

    1975-01-01

    Low levels of radionuclides were measured in Canada goose eggs taken from deserted nests from Columbia River islands on the Energy Research and Development Administration's Hanford Reservation. Potassium-40, a naturally occurring radionuclide, was the most abundant radionuclide measured in egg contents and egg shell. Strontium-90 was incorporated into egg shells and cesium-137 into inner egg contents. Manganese-54, cobalt-60, and zinc-65 were more abundant in inner egg contents than in egg shell. Cerium-144 was detected in egg shell but not in inner shell

  20. Using 3D printed eggs to examine the egg-rejection behaviour of wild birds

    Directory of Open Access Journals (Sweden)

    Branislav Igic

    2015-05-01

    Full Text Available The coevolutionary relationships between brood parasites and their hosts are often studied by examining the egg rejection behaviour of host species using artificial eggs. However, the traditional methods for producing artificial eggs out of plasticine, plastic, wood, or plaster-of-Paris are laborious, imprecise, and prone to human error. As an alternative, 3D printing may reduce human error, enable more precise manipulation of egg size and shape, and provide a more accurate and replicable protocol for generating artificial stimuli than traditional methods. However, the usefulness of 3D printing technology for egg rejection research remains to be tested. Here, we applied 3D printing technology to the extensively studied egg rejection behaviour of American robins, Turdus migratorius. Eggs of the robin’s brood parasites, brown-headed cowbirds, Molothrus ater, vary greatly in size and shape, but it is unknown whether host egg rejection decisions differ across this gradient of natural variation. We printed artificial eggs that encompass the natural range of shapes and sizes of cowbird eggs, painted them to resemble either robin or cowbird egg colour, and used them to artificially parasitize nests of breeding wild robins. In line with previous studies, we show that robins accept mimetically coloured and reject non-mimetically coloured artificial eggs. Although we found no evidence that subtle differences in parasitic egg size or shape affect robins’ rejection decisions, 3D printing will provide an opportunity for more extensive experimentation on the potential biological or evolutionary significance of size and shape variation of foreign eggs in rejection decisions. We provide a detailed protocol for generating 3D printed eggs using either personal 3D printers or commercial printing services, and highlight additional potential future applications for this technology in the study of egg rejection.

  1. Using 3D printed eggs to examine the egg-rejection behaviour of wild birds

    Science.gov (United States)

    Nunez, Valerie; Voss, Henning U.; Croston, Rebecca; Aidala, Zachary; López, Analía V.; Van Tatenhove, Aimee; Holford, Mandë E.; Shawkey, Matthew D.; Hauber, Mark E.

    2015-01-01

    The coevolutionary relationships between brood parasites and their hosts are often studied by examining the egg rejection behaviour of host species using artificial eggs. However, the traditional methods for producing artificial eggs out of plasticine, plastic, wood, or plaster-of-Paris are laborious, imprecise, and prone to human error. As an alternative, 3D printing may reduce human error, enable more precise manipulation of egg size and shape, and provide a more accurate and replicable protocol for generating artificial stimuli than traditional methods. However, the usefulness of 3D printing technology for egg rejection research remains to be tested. Here, we applied 3D printing technology to the extensively studied egg rejection behaviour of American robins, Turdus migratorius. Eggs of the robin’s brood parasites, brown-headed cowbirds, Molothrus ater, vary greatly in size and shape, but it is unknown whether host egg rejection decisions differ across this gradient of natural variation. We printed artificial eggs that encompass the natural range of shapes and sizes of cowbird eggs, painted them to resemble either robin or cowbird egg colour, and used them to artificially parasitize nests of breeding wild robins. In line with previous studies, we show that robins accept mimetically coloured and reject non-mimetically coloured artificial eggs. Although we found no evidence that subtle differences in parasitic egg size or shape affect robins’ rejection decisions, 3D printing will provide an opportunity for more extensive experimentation on the potential biological or evolutionary significance of size and shape variation of foreign eggs in rejection decisions. We provide a detailed protocol for generating 3D printed eggs using either personal 3D printers or commercial printing services, and highlight additional potential future applications for this technology in the study of egg rejection. PMID:26038720

  2. Egg capsules of the dusky catshark Bythaelurus canescens (Carcharhiniformes, Scyliorhinidae) from the south-eastern Pacific Ocean.

    Science.gov (United States)

    Concha, F; Bustamante, C; Oddone, M C; Hernández, S; Lamilla, J

    2010-09-01

    The external morphology of the egg capsule of Bythaelurus canescens and its fixation to the substratum are described. Bythaelurus canescens egg capsules are typically vase-shaped, dorso-ventrally flattened, pale yellow in colour when fresh and covered by 12-15 longitudinal ridges. The anterior border of the capsule is straight, whereas the posterior border is semicircular. Two horns bearing long, coiled tendrils arise from the anterior and posterior ends of the capsule. The presence of longitudinal ridges and long coiled tendrils at both anterior and posterior ends of the capsule readily distinguish these egg capsules from those of other chondrichthyans occurring in the south-east Pacific Ocean. © 2010 The Authors. Journal compilation © 2010 The Fisheries Society of the British Isles.

  3. Development of space-fertilized eggs and formation of primordial germ cells in the embryos of medaka fish

    Science.gov (United States)

    Ijiri, K.

    In the second International Microgravity Laboratory (IML-2) mission in 1994, four small Japanese killifish (Medaka, Oryzias latipes) made a space travel of 15 days aboard a space shuttle. These four adult Medaka fish successfully mated in space for the first time among vertebrate animals. Moreover, the eggs they laid developed normally, at least in their external appearance, hatching as fry (baby fish) in space. Fish mated and laid eggs every day during the first week. Near the end of the mission most of the eggs had a well-developed body with two pigmented eyes. In total, 43 eggs were laid (detected), out of which 8 fry hatched in space, as truly `space-originated' babies. A further 30 fry hatched within 3 days after landing. This is the normal hatching rate, compared with the ground-based data. Among the 8 space-originated fry, four were killed for histological sections, and germ cells at the gonadal region were counted for each fry. Their numbers were in the range of the germ cells of the normal control fry (ground-kept samples). Thus, as embryos developed normally in their external appearance, inside the embryos the formation of primordial germ cells took place normally in space, and their migration to the genital ridges was not hindered by microgravity. The two of the remaining space-originated fry have grown up and been creating their offspring in the laboratory. This proved that the primordial germ cells formed in space were also normal from a functional point of view. The four space-travelled adult fish re-started mating and laying eggs on the 7th day after landing and continued to do so every day afterward. Fertilization rate and hatchability of these eggs were as high as the eggs laid by the laboratory-kept fish. This fact implies that in gametogenesis of adult fish, there are no specific stages of germ cells extremely susceptible to microgravity.

  4. INFLUENCE OF TRIBULUS TERRESTRIS EXTRACT SUPPLEMENTATION ON LAYING PRODUCTIVITY AND EGGS QUALITY IN JAPANESE QUAILS

    Directory of Open Access Journals (Sweden)

    Martina Nickolova

    2011-01-01

    Full Text Available The aim of the current work was to examine the influence of Bulgarian phytoproduct VemoHerb T (dry extract of Tribulus terrestris –TT on laying productivity of Japanese quails (Coturnix coturnix japonica and their egg morphological and sensor properties. A trial was organized with 52 female and 16 male Japanese quails from the breed Faraon at the age of 44 days randomly divided in four groups – control and three experimental groups, 13 female and 4 male each. All birds were fed ad libitum the same compound feed for Japanese quails. The trial lasted 10 weeks. The experimental groups received with the drink water the tested product in following daily doses: 4mg/kg body weight (10weeks; 10mg/kg body weight (the first 5 weeks of the trial; 10mg/kg body weight (10 weeks for Ist, IInd , IIInd experimental groups respectively. The addition of TT-extract improved significantly the laying productivity. It was found significant higher values of egg weight, albumen - and yolk weight in quails from IInd and IIIrd experimental groups. There was a tendency to increase the egg shell weight and egg shell thickness in all treated groups in comparison to the control group. The usе of VemoHerb T did not aggravate the sensor properties of the quails’ eggs.

  5. Egg introduction: differential allergic responses

    OpenAIRE

    Dosanjh, Amrita

    2017-01-01

    Amrita Dosanjh Medical Center, Rady Childrens Hospital, San Diego, CA, USA Abstract: The use of egg protein preparations in clinical trials to reduce the incidence of egg allergy among infants includes a number of preparations of egg. These include whole egg, egg white protein, and egg yolk preparations. The study of the differential immune responses to these allergenic proteins in comparison is suggested as a future research area of investigation. Keywords: food allergy, egg allergy, clinica...

  6. Higher quality of molecular testing, an unfulfilled priority: Results from external quality assessment for KRAS mutation testing in colorectal cancer.

    Science.gov (United States)

    Tembuyser, Lien; Ligtenberg, Marjolijn J L; Normanno, Nicola; Delen, Sofie; van Krieken, J Han; Dequeker, Elisabeth M C

    2014-05-01

    Precision medicine is now a key element in clinical oncology. RAS mutational status is a crucial predictor of responsiveness to anti-epidermal growth factor receptor agents in metastatic colorectal cancer. In an effort to guarantee high-quality testing services in molecular pathology, the European Society of Pathology has been organizing an annual KRAS external quality assessment program since 2009. In 2012, 10 formalin-fixed, paraffin-embedded samples, of which 8 from invasive metastatic colorectal cancer tissue and 2 artificial samples of cell line material, were sent to more than 100 laboratories from 26 countries with a request for routine KRAS testing. Both genotyping and clinical reports were assessed independently. Twenty-seven percent of the participants genotyped at least 1 of 10 samples incorrectly. In total, less than 5% of the distributed specimens were genotyped incorrectly. Genotyping errors consisted of false negatives, false positives, and incorrectly genotyped mutations. Twenty percent of the laboratories reported a technical error for one or more samples. A review of the written reports showed that several essential elements were missing, most notably a clinical interpretation of the test result, the method sensitivity, and the use of a reference sequence. External quality assessment serves as a valuable educational tool in assessing and improving molecular testing quality and is an important asset for monitoring quality assurance upon incorporation of new biomarkers in diagnostic services. Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  7. Bacterial Colonization of Cod (Gadus morhua L.) and Halibut (Hippoglossus hippoglossus) Eggs in Marine Aquaculture

    OpenAIRE

    Hansen, Geir Høvik; Olafsen, Jan A.

    1989-01-01

    Aquaculture has brought about increased interest in mass production of marine fish larvae. Problems such as poor egg quality and mass mortality of fish larvae have been prevalent. The intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. Little information about the adherent microf...

  8. Maternal effects of egg size on emu Dromaius novaehollandiae egg composition and hatchling phenotype.

    Science.gov (United States)

    Dzialowski, Edward M; Sotherland, Paul R

    2004-02-01

    Parental investment in eggs and, consequently, in offspring can profoundly influence the phenotype, survival and ultimately evolutionary fitness of an organism. Avian eggs are excellent model systems to examine maternal allocation of energy translated through egg size variation. We used the natural range in emu Dromaius novaehollandiae egg size, from 400 g to >700 g, to examine the influence of maternal investment in eggs on the morphology and physiology of hatchlings. Female emus provisioned larger eggs with a greater absolute amount of energy, nutrients and water in the yolk and albumen. Variation in maternal investment was reflected in differences in hatchling size, which increased isometrically with egg size. Egg size also influenced the physiology of developing emu embryos, such that late-term embryonic metabolic rate was positively correlated with egg size and embryos developing in larger eggs consumed more yolk during development. Large eggs produced hatchlings that were both heavier (yolk-free wet and dry mass) and structurally larger (tibiotarsus and culmen lengths) than hatchlings emerging from smaller eggs. As with many other precocial birds, larger hatchlings also contained more water, which was reflected in a greater blood volume. However, blood osmolality, hemoglobin content and hematocrit did not vary with hatchling mass. Emu maternal investment in offspring, measured by egg size and composition, is significantly correlated with the morphology and physiology of hatchlings and, in turn, may influence the success of these organisms during the first days of the juvenile stage.

  9. 21 CFR 160.180 - Egg yolks.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Egg yolks. 160.180 Section 160.180 Food and Drugs... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.180 Egg yolks. (a) Egg yolks, liquid egg yolks, yolks, liquid yolks are yolks of eggs of the domestic hen so...

  10. Estimating the Number of Eggs in Blow Fly (Diptera: Calliphoridae) Egg Masses Using Photographic Analysis.

    Science.gov (United States)

    Rosati, J Y; Pacheco, V A; Vankosky, M A; Vanlaerhoven, S L

    2015-07-01

    Little work has been done to quantify the number of eggs oviposited by blow flies (Diptera: Calliphoridae) in studies examining colonization behavior. Egg counting methods currently available are time-consuming and destructive. This study used ImageJ software and analysis of covariance to relate the volume of egg masses to the number of eggs laid by three different blow fly species: Lucilia sericata (Meigen), Phormia regina (Meigen), and Chrysomya rufifacies (Macquart). Egg mass volume, species, and the interaction of species and egg mass volume all affected the number of blow fly eggs deposited in egg masses. Both species identity and egg mass volume are important when predicting egg number, as such a single regression equation cannot be used to estimate egg number for these three species. Therefore, simple linear regression equations were determined for each species. The volume of individual eggs was incorporated into the model, yet differences between species were observed, suggesting that the orientation of the eggs oviposited by multiple conspecific females within egg masses influences egg estimates. Based on our results, we expect that imaging software can be used for other blow fly species, as well as other insect species; however, equations specific to each species must be developed. This study describes an important tool for quantifying egg deposition in a nondestructive manner, which is important in studying the colonization behavior and life history of insects of ecological and forensic importance. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  11. Effect of dietary supplementation of Ligustrum lucidum on performance, egg quality and blood biochemical parameters of Hy-Line Brown hens during the late laying period.

    Science.gov (United States)

    Li, X L; He, W L; Yang, M L; Yan, Y M; Xue, Y H; Zhao, S T

    2017-11-01

    The fruit of Ligustrum lucidum (FLL, Nuzhenzi in Chinese) is an important traditional medicine, and have attracted significant research attention because of their various biological activities. However, there are few research reports available on the use of FLL as a feed additive in livestock nutrition, particularly in layers. This study was conducted to determine the effects of supplementation of the diet of laying hens with FLL on laying performance, egg quality and blood metabolites. A total of 360 72-week-old hens were allocated to three dietary treatments (eight replications of 15 hens/treatment group) and were fed either a control diet or a diet supplemented with an inclusion level of 0.25% or 0.50% of FLL powder in the final feed, until 78 weeks of age. Hens were housed in a three-tier cage system. Feed and water were provided ad libitum. Blood samples and eggs were collected at the end of the experiment. The results showed that dietary supplementation with FLL did not affect egg weight, feed conversion ratio, eggshell thickness, albumen height, egg yolk color, eggshell breaking strength or egg shape index. However, FLL supplementation significantly decreased (Phens fed FLL compared with the control group. It can be concluded that FLL, at a supplementation level of 0.25% final feed, can be used as an effective feed additive to improve the performance of laying hens during the late laying period.

  12. Enumeration of Salmonellae in Table Eggs, Pasteurized Egg Products, and Egg-Containing Dishes by Using Quantitative Real-Time PCR

    DEFF Research Database (Denmark)

    Jakočiūnė, Džiuginta; Pasquali, Frédérique; da Silva, Cristiana Soares

    2014-01-01

    PCR) was employed for enumeration of salmonellae in different matrices: table eggs, pasteurized egg products, and egg-containing dishes. Salmonella enterica serovar Enteritidis and S. enterica serovar Tennessee were used to artificially contaminate these matrices. The results showed a linear regression between...... the numbers of salmonellae and the quantification cycle (Cq) values for all matrices used, with the exception of pasteurized egg white. Standard curves were constructed by using both stationary-phase cells and heat-stressed cells, with similar results. Finally, this method was used to evaluate the fate...

  13. Dipping of Eggs, Use of Low Temperature and Aeration to Improve Fruit Fly Bactrocera Carambolae (Drew and Hancock) Mass Rearing in Sterile Insect Technique Programme

    International Nuclear Information System (INIS)

    Nasution, Indah Arastuti; Achmad Nasroh Kuswadi

    2004-01-01

    In the Sterile Insect Technique (SIT) programme a large number of homogenous age of pupae should be produced to be sterilized and released into the field. A methodology to preserve eggs which at the same time delay the egg hatch is needed in the production of a large number of pupae with homogenous age. The study on method of preserving fruit fly eggs the effect of dipping, low temperature, on the viability of eggs, the quality of larvae hatched from the eggs, and the quality of pupae produced were observed. Viability of eggs were observed as percent of egg hatch, quality of larvae as number of pupae produced from 0.5 ml of eggs when reared on artificial diet, and quality of pupae as percent of the flies number emerged and flew from the pupae. In room temperature (26 o C) dipping did not preserve fruit fly eggs and aeration did not improve the preservation. Although after one day dipping the viability of eggs only decreased from 90 % (control) to 80.5 %, (without aeration) and 81.5 % (with aeration) it decreased the number of pupae been produced from 1447 pupae (control) to 382 pupae (without aeration) and 616 pupae (with aeration). Low temperature successfully preserved the egg up to 24 hours. After 24 hour dipping in low temperature (16 o C) the viability of eggs were decreased up to 84.75 % (without aeration) and able to produce 1104 pupae. Aeration during dipping in low temperature did not improve the preservation. (author)

  14. Campylobacter jejuni in commercial eggs.

    Science.gov (United States)

    Fonseca, Belchiolina Beatriz; Beletti, Marcelo Emílio; de Melo, Roberta Torres; Mendonça, Eliane Pereira; Coelho, Letícia Ríspoli; Nalevaiko, Priscila Christen; Rossi, Daise Aparecida

    2014-01-01

    This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.

  15. Carbohydrase and phytase supplementation in diets for semi-heavy laying hens

    Directory of Open Access Journals (Sweden)

    Adriano Geraldo

    2014-07-01

    Full Text Available This study was conducted in order to evaluate the association of phytase with an enzymatic complex comprised of carbohydrases (α-galactosidase, galactomannan, xylanase and β-glucanase in nutrition reduction diets for semi-heavy laying hens and its effect on egg performance and egg quality. Four hundred Isa Brown laying hens with 42 to 57 weeks of age were distributed in an entirely random experiment with five treatments and 8 repetitions, during five production periods of 21 days. Variables studied: egg production, feed intake, mean egg weight, feed conversion, Haugh unit, percentage of yolk, egg white and albumen, yolk color, eggshell thickness and specific gravity. There was a significant interaction (p 0.05 of treatment on production, egg weight or internal and external egg quality. Treatment effects on feed conversion showed better values for hens fed with the control diet. The levels of nutrient reduction used in the diets with or without enzyme supplementation did not provide good results with regard to feed conversion and feed intake. However, they did not affect the other parameters for egg production and internal and external egg quality.

  16. Egg number-egg size: an important trade-off in parasite life history strategies.

    Science.gov (United States)

    Cavaleiro, Francisca I; Santos, Maria J

    2014-03-01

    Parasites produce from just a few to many eggs of variable size, but our understanding of the factors driving variation in these two life history traits at the intraspecific level is still very fragmentary. This study evaluates the importance of performing multilevel analyses on egg number and egg size, while characterising parasite life history strategies. A total of 120 ovigerous females of Octopicola superba (Copepoda: Octopicolidae) (one sample (n=30) per season) were characterised with respect to different body dimensions (total length; genital somite length) and measures of reproductive effort (fecundity; mean egg diameter; total reproductive effort; mean egg sac length). While endoparasites are suggested to follow both an r- and K-strategy simultaneously, the evidence found in this and other studies suggests that environmental conditions force ectoparasites into one of the two alternatives. The positive and negative skewness of the distributions of fecundity and mean egg diameter, respectively, suggest that O. superba is mainly a K-strategist (i.e. produces a relatively small number of large, well provisioned eggs). Significant sample differences were recorded concomitantly for all body dimensions and measures of reproductive effort, while a general linear model detected a significant influence of season*parasite total length in both egg number and size. This evidence suggests adaptive phenotypic plasticity in body dimensions and size-mediated changes in egg production. Seasonal changes in partitioning of resources between egg number and size resulted in significant differences in egg sac length but not in total reproductive effort. Evidence for a trade-off between egg number and size was found while controlling for a potential confounding effect of parasite total length. However, this trade-off became apparent only at high fecundity levels, suggesting a state of physiological exhaustion. Copyright © 2014 Australian Society for Parasitology Inc. Published

  17. Conspecific brood parasitism and egg quality in blue tits Cyanistes caeruleus

    NARCIS (Netherlands)

    Vedder, Oscar; Kingma, Sjouke-Anne; Engelhardt, Nikolaus von; Korsten, Peter; Groothuis, Ton G.G.; Komdeur, Jan

    Laying eggs in nests of unrelated conspecific pairs to parasitize their parental care is a common phenomenon in birds. In blue tits Cyanistes caeruleus such conspecific brood parasitism (CBP) has never been reported in the literature. However, in a situation where breeding density was extremely

  18. 9 CFR 590.45 - Prohibition on eggs and egg products not intended for use as human food.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Prohibition on eggs and egg products... INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EGG PRODUCTS INSPECTION INSPECTION OF EGGS AND EGG PRODUCTS (EGG PRODUCTS INSPECTION ACT) Eggs and Egg Products Not Intended for Human Food § 590.45 Prohibition on...

  19. Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays.

    Science.gov (United States)

    Staats, Janet S; Enzor, Jennifer H; Sanchez, Ana M; Rountree, Wes; Chan, Cliburn; Jaimes, Maria; Chan, Ray Chun-Fai; Gaur, Amitabh; Denny, Thomas N; Weinhold, Kent J

    2014-07-01

    The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular Cytokine Staining (ICS) assays. The goal of the EQAPOL Flow Cytometry External Quality Assurance Program (EQAP) is to provide proficiency testing and remediation for participating sites. The program is not punitive; rather, EQAPOL aims to help sites identify areas for improvement. EQAPOL utilizes a highly standardized ICS assay to minimize variability and readily identify those sites experiencing technical difficulties with their assays. Here, we report the results of External Proficiency 3 (EP3) where participating sites performed a 7-color ICS assay. On average, sites perform well in the Flow Cytometry EQAP (median score is "Good"). The most common technical issues identified by the program involve protocol adherence and data analysis; these areas have been the focus of site remediation. The EQAPOL Flow Cytometry team is now in the process of expanding the program to 8-color ICS assays. Evaluating polyfunctional ICS responses would align the program with assays currently being performed in support of HIV immune monitoring assays. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Effect of location of eggs in the incubator on hatchability of eggs from ...

    African Journals Online (AJOL)

    ... the hatchability of all eggs set and of fertile eggs was the highest in eggs placed in the front of the machine. Furthermore, early and middle stage deaths during incubation were lower in the front of the incubator compared to the back. The differences between eggs placed in the upper, middle and lower parts of the machine ...

  1. External quality assurance performance of clinical research laboratories in sub-saharan Africa.

    Science.gov (United States)

    Amukele, Timothy K; Michael, Kurt; Hanes, Mary; Miller, Robert E; Jackson, J Brooks

    2012-11-01

    Patient Safety Monitoring in International Laboratories (JHU-SMILE) is a resource at Johns Hopkins University that supports and monitors laboratories in National Institutes of Health-funded international clinical trials. To determine the impact of the JHU-SMILE quality assurance scheme in sub-Saharan African laboratories, we reviewed 40 to 60 months of external quality assurance (EQA) results of the College of American Pathologists (CAP) in these laboratories. We reviewed the performance of 8 analytes: albumin, alanine aminotransferase, creatinine, sodium, WBC, hemoglobin, hematocrit, and the human immunodeficiency virus antibody rapid test. Over the 40- to 60-month observation period, the sub-Saharan laboratories had a 1.63% failure rate, which was 40% lower than the 2011 CAP-wide rate of 2.8%. Seventy-six percent of the observed EQA failures occurred in 4 of the 21 laboratories. These results demonstrate that a system of remote monitoring, feedback, and audits can support quality in low-resource settings, even in places without strong regulatory support for laboratory quality.

  2. Perceived external prestige as a mediator between quality of work life and organisational commitment of public sector employees in Ghana

    OpenAIRE

    Ojedokun, Oluyinka; Idemudia, Erhabor S.; Desouza, Mercy

    2015-01-01

    Orientation: Research efforts have been directed at understanding the relationship between quality of work life and organisational commitment, but these studies have not elucidated the mediating role of perceived external prestige in this relationship. Research purpose: This research seeks to close a research gap by determining the role of perceived external prestige in the relationship between quality of work life and organisational commitment amongst public sector employees in Ghana. ...

  3. Egg CD9 protein tides correlated with sperm oscillations tune the gamete fusion ability in mammal.

    Science.gov (United States)

    Ravaux, Benjamin; Favier, Sophie; Perez, Eric; Gourier, Christine

    2018-01-23

    Mammalian fertilization involves membrane events -adhesion, fusion, sperm engulfment, membrane block to polyspermy- whose causes remain largely unknown. Recently, specific oscillations of the sperm in contact with the egg were shown to be necessary for fusion. Using a microfluidic chip to impose the venue for the encounter of two gametes allowed real-time observation of the membrane remodelling occurring at the sperm/egg interface. The spatiotemporal mapping of egg CD9 revealed that this protein concentrates at the egg/sperm interface as a result of sperm oscillations, until a CD9-rich platform is nucleated on which fusion immediately takes place. Within 2 to 5 minutes after fusion, most of the CD9 leaves the egg for the external aqueous medium. Then an egg membrane wave engulfs the sperm head in approximately 25 minutes. These results show that sperm oscillations initiate the CD9 recruitment that causes gamete fusion after which CD9 and associated proteins leave the membrane in a process likely to contribute to block polyspermy. They highlight that the gamete fusion story in mammals is an unexpected interplay between mechanical constraints and proteins. © The Author(s) (2018). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. All rights reserved.

  4. 9 CFR 590.920 - Importer to make application for inspection of imported eggs and egg products.

    Science.gov (United States)

    2010-01-01

    ... inspection of imported eggs and egg products. 590.920 Section 590.920 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EGG PRODUCTS INSPECTION INSPECTION OF EGGS AND EGG PRODUCTS (EGG PRODUCTS INSPECTION ACT) Imports § 590.920 Importer to make application for inspection of...

  5. Diet composition and quality for Calanus finmarchicus egg production and hatching success off south-west Iceland

    DEFF Research Database (Denmark)

    Jonasdottir, Sigrun; Gudfinnsson, H.G.; Gislason, A.

    2002-01-01

    Egg production and hatching success of the copepod Calanus finmarchicus was measured during spring and summer in the waters south-west of Iceland. Egg-production rates varied greatly, both temporally and spatially, with highest average rates found at a station with low chlorophyll-a concentrations...

  6. 9 CFR 590.510 - Classifications of shell eggs used in the processing of egg products.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Classifications of shell eggs used in the processing of egg products. 590.510 Section 590.510 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EGG PRODUCTS INSPECTION INSPECTION OF EGGS AND EGG PRODUCTS...

  7. Trichuris trichiura egg (image)

    Science.gov (United States)

    ... is the classical appearance of the Trichuria (whipworm) egg. The eggs are highly infectious. After a person eats contaminated food, the worms hatch from the eggs and live in the intestine, causing vomiting and ...

  8. Determinants of Quality Perception in Educational Administration: Potential Conflict between the Requirements of Internal and External Customers.

    Science.gov (United States)

    Galloway, R. L.; Wearn, Katrina

    1998-01-01

    Attempts to measure perceived quality, based on customers' views within the context of a (British) university faculty office, using a modified SERVQUAL instrument. Internal customers demonstrated that perceived quality is driven by task-focused issues, stressing clarity, accuracy, and reliability. External customers, whose usage is more casual,…

  9. Geographical variation in egg mass and egg content in a passerine bird.

    Directory of Open Access Journals (Sweden)

    Suvi Ruuskanen

    Full Text Available Reproductive, phenotypic and life-history traits in many animal and plant taxa show geographic variation, indicating spatial variation in selection regimes. Maternal deposition to avian eggs, such as hormones, antibodies and antioxidants, critically affect development of the offspring, with long-lasting effects on the phenotype and fitness. Little is however known about large-scale geographical patterns of variation in maternal deposition to eggs. We studied geographical variation in egg components of a passerine bird, the pied flycatcher (Ficedula hypoleuca, by collecting samples from 16 populations and measuring egg and yolk mass, albumen lysozyme activity, yolk immunoglobulins, yolk androgens and yolk total carotenoids. We found significant variation among populations in most egg components, but ca. 90% of the variation was among individuals within populations. Population however explained 40% of the variation in carotenoid levels. In contrast to our hypothesis, we found geographical trends only in carotenoids, but not in any of the other egg components. Our results thus suggest high within-population variation and leave little scope for local adaptation and genetic differentiation in deposition of different egg components. The role of these maternally-derived resources in evolutionary change should be further investigated.

  10. Replacing Corn and Wheat in Layer Diets with Hulless Oats Shows Effects on Sensory Properties and Yolk Quality of Eggs

    Directory of Open Access Journals (Sweden)

    Louisa R. Winkler

    2017-07-01

    Full Text Available US organic poultry producers are under pressure to find feed alternatives to corn and wheat. Hulless oats offer advantages such as wide geographic adaptation of the plant and high concentrations of protein and oil in the grain. They have shown considerable potential in experimental work as a feed grain for poultry, but more research is needed into their influence on the sensory and nutritional properties of eggs. In this study, hulless oats were substituted for corn or wheat at 200 g kg−1 in diets fed to Hy-Line Brown hens and eggs were sampled for sensory evaluation after 8 weeks. Discrimination tests of blended and baked egg samples found evidence of difference between eggs from oat-based diets and those from the oat-free control (p < 0.05 for eggs from an oat-corn diet, p < 0.01 for eggs from an oat-wheat diet. Acceptance tests of similar samples showed that eggs from the oat-wheat diet were significantly less liked than control eggs for their texture (p < 0.01 and response to cooking (p < 0.01, while eggs from the oat-corn diet were somewhat less liked. Yolk weight was greater (p < 0.05 in control eggs (34.1 g than eggs from oat-corn (31.6 g or oat-wheat (31.2 g diets, leading to smaller yolk proportion in the oat-fed eggs. Fatty acid profile differences across treatments were not of nutritional significance, and no evidence was found that the feeding of hulless oats improved storage properties of eggs. In this study, modifying the carbohydrate source in layer diets was shown to change textural properties of cooked eggs in a way that was perceptible to untrained consumers, probably by reducing the yolk proportion. This finding was not commercially relevant owing to small effect size, and results overall add to existing evidence that hulless oats can be fed to poultry at a moderate proportion of the diet with no negative effect on consumer acceptability of eggs. Regardless of the small effect size, however

  11. Sublethal Exposure to Diatomaceous Earth Increases Net Fecundity of Flour Beetles (Tribolium confusum) by Inhibiting Egg Cannibalism

    Science.gov (United States)

    Shostak, Allen W.

    2014-01-01

    Population regulation results from an interplay of numerous intrinsic and external factors, and for many insects cannibalism is such a factor. This study confirms a previously-reported observation that sublethal exposure to the fossilized remains of diatoms (i.e. diatomaceous earth) increases net fecundity (eggs produced minus eggs destroyed/day) of flour beetles, Tribolium confusum. The aim was to experimentally test two non-mutually-exclusive ecological mechanisms potentially responsible for the increased net fecundity: higher egg production and lower egg cannibalism. Adult T. confusum were maintained at low or high density in medium containing sublethal (0–4%) diatomaceous earth. Net fecundity increased up to 2.1× control values during diatomaceous earth exposure, and returned to control levels following removal from diatomaceous earth. Cannibalism assays on adults showed that diatomaceous earth reduced the number of eggs produced to 0.7× control values at low density and to 0.8× controls at high density, and also reduced egg cannibalism rates of adults to as little as 0.4× control values, but at high density only. Diatomaceous earth also reduced cannibalism by larvae on eggs to 0.3× control values. So, while the presence of diatomaceous earth reduced egg production, net fecundity increased as a result of strong suppression of the normal egg cannibalism by adults and larvae that occurs at high beetle density. Undisturbed cultures containing sublethal diatomaceous earth concentrations reached higher population densities than diatomaceous earth-free controls. Cohort studies on survival from egg to adult indicated that this population increase was due largely to decreased egg cannibalism by adult females. This is the first report of inhibition of egg cannibalism by diatomaceous earth on larval or adult insects. The ability of diatomaceous earth to alter cannibalism behavior without causing mortality makes it an ideal investigative tool for cannibalism

  12. Assessing the Organisational Impact of External Quality Assurance: Hypothesising Key Dimensions and Mechanisms

    Science.gov (United States)

    Stensaker, Bjørn; Leiber, Theodor

    2015-01-01

    The aim of the article is to provide a framework in which the organisational impact of external quality assurance (EQA) can be assessed. Based on existing studies of the impact of EQA in universities and colleges it is suggested that greater systematisation of how impact is measured is needed for a better understanding of how EQA can be used as a…

  13. [Indication for mitral valve interventions: Development of a quality indicator for external hospital quality assurance].

    Science.gov (United States)

    Köster, Christina; Schorbach, Lena; Wrede, Stephanie; Meyer, Sven; Kazmaier, Tonia; Szecsenyi, Joachim

    2017-11-01

    The indication for a mitral valve intervention is an important patient-relevant parameter for the assessment of process quality and the comparison of healthcare providers. In this article, we describe the development of a corresponding quality indicator for an external hospital quality assurance (QA) procedure in Germany. An expert panel was set up by the aQua Institute to assist with the development of a QA procedure for mitral valve interventions and the associated quality indicators. In a comprehensive, systematic literature and evidence research, the American and European guidelines were identified as the best evidence available. Especially the more current American guideline formed the basis on which a quality indicator dealing with the correct indication for a mitral valve intervention was developed. The developed quality indicator assesses the proportion of patients for whom an indication for a mitral valve intervention was determined in compliance with guideline recommendations. The indicator differentiates between surgical and catheter-based procedures. To determine whether or not the indication was correct, different medical parameters are included, such as, for example, type of mitral valve defect, etiology of the disease, severity of symptoms, valve morphology (e. g., mitral valve area), valve hemodynamics and comorbidity, which healthcare providers have to document. The documentation for the developed quality indicator is considerable. Nonetheless, its relevance is undeniable because it allows the user to determine whether a surgical or catheter-based mitral valve intervention was necessary and performed according to guideline recommendations. In the first year of its implementation, this indicator should be evaluated for further improvement and simplification of assessment. Copyright © 2017. Published by Elsevier GmbH.

  14. Odd-Boiled Eggs

    Science.gov (United States)

    Kaminsky, Kenneth; Scheman, Naomi

    2010-01-01

    At a Shabbat lunch in Madrid not long ago, the conversation turned to the question of boiling eggs. One of the guests mentioned that a Dutch rabbi he knew had heard that in order to make it more likely that boiled eggs be kosher, you should add an egg to the pot if the number you began with was even. According to the laws of Kashruth, Jews may not…

  15. Effects of salinity and sea salt type on egg activation, fertilization, buoyancy and early embryology of European eel, Anguilla anguilla

    DEFF Research Database (Denmark)

    Sørensen, Sune Riis; Butts, Ian; Munk, Peter

    2016-01-01

    sizes, while the remaining four salt types resulted in smaller eggs. All salt types except NaCl treatments led to high fertilization rates and had no effect on fertilization success as well as egg neutral buoyancies at 7 h post-fertilization. The study points to the importance of considering ionic...... and egg buoyancy. Egg diameter after activation, using natural seawater adjusted to different salinities, varied among female eels, but no consistent pattern emerged. Activation salinities between 30–40 practical salinity unit (psu) produced higher quality eggs and generally larger egg diameters. Chorion...

  16. 21 CFR 160.145 - Dried egg whites.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Dried egg whites. 160.145 Section 160.145 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.145 Dried egg whites. (a) The food dried egg whites, egg white solids, dried egg albumen, egg albumen solids is...

  17. "Ant-egg" cataract revisited

    DEFF Research Database (Denmark)

    Clemmensen, Kåre; Enghild, Jan J; Ivarsen, Anders

    2017-01-01

    -ray scans and electron microscopy. The purpose of this study was to further characterize "ant-egg" cataract using modern technology and display the history of the "ant-eggs" after cataract extraction. METHODS: "Ant-eggs" were examined using Heidelberg SPECTRALIS Optical Coherence Tomography (OCT...

  18. EGG: Empirical Galaxy Generator

    Science.gov (United States)

    Schreiber, C.; Elbaz, D.; Pannella, M.; Merlin, E.; Castellano, M.; Fontana, A.; Bourne, N.; Boutsia, K.; Cullen, F.; Dunlop, J.; Ferguson, H. C.; Michałowski, M. J.; Okumura, K.; Santini, P.; Shu, X. W.; Wang, T.; White, C.

    2018-04-01

    The Empirical Galaxy Generator (EGG) generates fake galaxy catalogs and images with realistic positions, morphologies and fluxes from the far-ultraviolet to the far-infrared. The catalogs are generated by egg-gencat and stored in binary FITS tables (column oriented). Another program, egg-2skymaker, is used to convert the generated catalog into ASCII tables suitable for ingestion by SkyMaker (ascl:1010.066) to produce realistic high resolution images (e.g., Hubble-like), while egg-gennoise and egg-genmap can be used to generate the low resolution images (e.g., Herschel-like). These tools can be used to test source extraction codes, or to evaluate the reliability of any map-based science (stacking, dropout identification, etc.).

  19. Eggs on Ice. Imaginaries on Eggs and Cryopreservation in Denmark

    DEFF Research Database (Denmark)

    Herrmann, Janne Rothmar; Kroløkke, Charlotte

    2018-01-01

    While Denmark is widely known as a global exporter of cryopreserved sperm, Danish women’s eggs follow very different trajectories. This paper combines legal and rhetorical analyses with the concept of sociotechnical imaginaries (Jasanoff, 2015). In establishing the genealogy of the sociotechnical...... imaginaries that shaped the Danish regulation on the cryopreservation of eggs, we analyze the relevant Acts, Bills, preparatory work and readings in Parliament along with the concurrent public and ethical debates that in time relaxed the legal limit for the cryopreservation of eggs to the current 5 years...... and today continue to ignite discussions on elective egg freezing. We rely on welfare state perspectives to discuss why reproduction, in the Danish context, is seen as a legitimate and appropriate sphere to regulate and we turn to feminist theorizing to discuss their gendered implications captured...

  20. Assessment of welfare and egg production of laying hens moravia ssl in small-scale breeding

    Directory of Open Access Journals (Sweden)

    Mária Angelovičová

    2015-10-01

    Full Text Available The purpose of this study was to investigate the welfare of laying hens Moravia SSL housed in small-scale hen house with free range, behavior, egg production and selected physical indicators of eggs and chemical indicators of egg mass. The laying hens were kept in a hen house on deep litter. Breeding facility of hen house was within the meaning of recommendation for applying the principles of welfare, i.e. the space and breeding facility within the meaning of enriched breeding environment. Stocking density of the laying hens corresponded with recommendations for unrestricted movement and implementing natural activities. The hen house was equipped with the perch, nest, feeder and drinker. The commercial feed mixture was used for feeding, which is intended for laying hens. The kitchen remains were added to feed mixture, as are wet bread, the non-edible remains of foodstuffs. A feed mixture was served to laying hens 825 g per day. The laying hens had free access to drinking water, grazing, ground pecking, ground scratching and dust-bathing and in the free range. We focused investigation of on the egg laying intensity, selected parameters of physical egg quality and chemical egg contents. Time to relax of laying hens was adjusted according to the summer and winter breeding seasons. The main activities of free-range hens are grazing, ground pecking, ground scratching and dust-bathing. The main activities of free-range hens are grazing, ground pecking, ground scratching and dust-bathing. These activities were investigated in laying hens too in dependent of year period, more in the summer. Housing of the hens was equipped with the perch. The laying hens regularly used a perch. A beginning of occupation the perch was at the time of time growing dark, at the end of the light day. A nesting material was selected regular, monthly exchange. It was meadow hay of excellent quality for the collection of high quality and safe eggs from nests in the hen house

  1. Child Reactivity Moderates the Over-Time Association between Mother-Child Conflict Quality and Externalizing Problems

    Science.gov (United States)

    Nelson, Jackie A.

    2015-01-01

    Constructive parent-child conflict interactions that teach children to problem-solve and negotiate can enhance children's social adjustment. This paper identifies constructive and destructive qualities of mother-child conflict and explores whether child temperament moderated associations with changes in externalizing problems over time. One…

  2. Copepod recruitment and food composition : Do diatoms affect hatching success?

    DEFF Research Database (Denmark)

    Jonasdottir, Sigrun; Kiørboe, Thomas

    1996-01-01

    was fed several different diets while egg production and hatching success were monitored. The diet was analysed for fatty acid content as an indicator of food quality. Both egg production and hatching were found to be affected by the nutritional quality of the food. Hatching was also highly dependent......Laboratory experiments were conducted to differentiate between factors controlling the hatching success of copepod eggs. Factors that could affect viability of eggs; viz food quality, female condition and external factors were investigated. In a series of experiments the copepod Acartia tonsa Dana...

  3. Effect of egg washing and correlation between cuticle and egg penetration by various Salmonella strains.

    Science.gov (United States)

    Gole, Vaibhav C; Roberts, Juliet R; Sexton, Margaret; May, Damian; Kiermeier, Andreas; Chousalkar, Kapil K

    2014-07-16

    In Australia, Europe and the United States, eggs and egg products are frequently associated with Salmonella food poisoning outbreaks. Many of the egg-associated Salmonella outbreaks have been due to the products such as mayonnaise, ice-cream and cold desserts which are eaten without cooking following the addition of raw egg. The ability of four Salmonella isolates (one each of S. Singapore, S. Adelaide, S. Worthington and S. Livingstone) to penetrate washed and unwashed eggs using whole egg and agar egg penetration methods was investigated in the current study. The results of the agar penetration experiment indicated that all the isolates used in the present study have the capacity to penetrate the eggshell. Eggshell penetration by the S. Worthington isolate was higher but not significant (p=0.06) in washed eggs compared to unwashed eggs. However, for all other isolates (S. Singapore, S. Adelaide and S. Livingstone), there was no significant difference in penetration of washed and unwashed eggs. Statistical analysis indicated that cuticle score was a significant linear predictor of Salmonella eggshell penetration. Whole egg penetration results showed that all of the Salmonella isolates used in the present study were capable of surviving on the eggshell surface after 21days of incubation (at 20°C) following a high dose of inoculation (10(5)CFU/mL). The combined data of all isolates demonstrated that, the survival rate of Salmonella on eggshells (inoculated with 10(5)CFU/mL) was significantly higher (p=0.002) at 20°C as compared to 37°C. S. Singapore, S. Worthington, and S. Livingstone were not detected in egg internal contents whereas S. Adelaide was detected in one egg's internal contents. Crown Copyright © 2014. Published by Elsevier B.V. All rights reserved.

  4. Desempenho e qualidade dos ovos de poedeiras semipesadas alimentadas com dietas contendo óleos de soja e canola Performance and eggs quality in laying hens fed diets with soybean and canola oils

    Directory of Open Access Journals (Sweden)

    Fernando Guilherme Perazzo Costa

    2008-08-01

    Full Text Available Objetivou-se analisar a influência da adição de níveis crescentes de óleo de soja e canola sobre os índices de desempenho e qualidade interna e externa dos ovos de poedeiras comerciais semipesadas da linhagem Bovans Goldline durante cinco períodos de 28 dias. Foram utilizadas 280 aves com 18 semanas de idade, em um delineamento inteiramente casualizado, com sete tratamentos em um esquema fatorial 2 × 3 + 1 (dois tipos de óleo e três níveis de óleo mais um testemunha adicional com cinco repetições e oito aves por unidade experimental. Os níveis de óleo de soja e de canola não alteraram o consumo de ração, os pesos dos ovos, de albúmen, de gema e de casca, as porcentagens de albúmen, de gema e de casca e a gravidade específica dos ovos. Houve influência significativa da interação tipo × nível de óleo sobre a produção de ovos e a conversão por massa e por dúzia de ovos. Com o aumento do nível de óleo de soja, os resultados obtidos para estas variáveis melhoraram, entretanto, a conversão por massa de ovo piorou com o aumento dos níveis de óleo de canola. A adição de óleo de soja promoveu desempenho melhor que o obtido com óleo de canola.The objective of this study was to evaluate the influence of soybean and canola oil added in crescent levels on production performance indexes and internal and external egg quality of brown commercial layers of the strain Bovans Goldline during five periods of 28 days. Two hundred and eighty hens with 18 weeks old were distributed in a completely randomized design, with seven diets in a 2 × 3 + 1 factorial arrangement (oil type and oil level, and an additional control, with 5 replicates of 8 hens per experimental unit. The soybean and canola oil levels did not affect the feed consumption; egg, albumen, yolk and shell weights; albumen, yolk and shell percentages, neither the specific gravity. There was an interaction between type and oils levels on egg production and mass

  5. Vision Technology for Automated Characterization of Parasite Eggs in a Medicinal Product

    DEFF Research Database (Denmark)

    Bruun, Johan Musaeus

    Eggs from the pig whipworm, Trichuris suis ova, constitute the active pharmaceutical ingredient in a drug candidate targeting immune-mediated diseases such as Crohn’s disease, multiple sclerosis, a.o. The drug candidate is currently being evaluated in clinical trials around the world. In the pres......Eggs from the pig whipworm, Trichuris suis ova, constitute the active pharmaceutical ingredient in a drug candidate targeting immune-mediated diseases such as Crohn’s disease, multiple sclerosis, a.o. The drug candidate is currently being evaluated in clinical trials around the world....... In the present PhD project, a microscopy-based vision instrument has been developed to automate the characterization of egg suspension quantity and quality. The instrument uses digital image analysis and statistical classification to detect and count the eggs and to characterize the developmental stages...... of the larvae inside the eggs based on morphology and darkfield scattering. The novel application of this technology is described in a patent application. The assessed concentration and embryonation percentage is used in the preparation of medicinal doses for the patients. The instrument has been evaluated...

  6. Environmental impacts and sustainability of egg production systems.

    Science.gov (United States)

    Xin, H; Gates, R S; Green, A R; Mitloehner, F M; Moore, P A; Wathes, C M

    2011-01-01

    As part of a systemic assessment toward social sustainability of egg production, we have reviewed current knowledge about the environmental impacts of egg production systems and identified topics requiring further research. Currently, we know that 1) high-rise cage houses generally have poorer air quality and emit more ammonia than manure belt (MB) cage houses; 2) manure removal frequency in MB houses greatly affects ammonia emissions; 3) emissions from manure storage are largely affected by storage conditions, including ventilation rate, manure moisture content, air temperature, and stacking profile; 4) more baseline data on air emissions from high-rise and MB houses are being collected in the United States to complement earlier measurements; 5) noncage houses generally have poorer air quality (ammonia and dust levels) than cage houses; 6) noncage houses tend to be colder during cold weather due to a lower stocking density than caged houses, leading to greater feed and fuel energy use; 7) hens in noncage houses are less efficient in resource (feed, energy, and land) utilization, leading to a greater carbon footprint; 8) excessive application of hen manure to cropland can lead to nutrient runoff to water bodies; 9) hen manure on open (free) range may be subject to runoff during rainfall, although quantitative data are lacking; 10) mitigation technologies exist to reduce generation and emission of noxious gases and dust; however, work is needed to evaluate their economic feasibility and optimize design; and 11) dietary modification shows promise for mitigating emissions. Further research is needed on 1) indoor air quality, barn emissions, thermal conditions, and energy use in alternative hen housing systems (1-story floor, aviary, and enriched cage systems), along with conventional housing systems under different production conditions; 2) environmental footprint for different US egg production systems through life cycle assessment; 3) practical means to mitigate air

  7. Effects of Hen Age and Egg Weight Class on the Hatchability of Free Range Indigenous Chicken Eggs

    OpenAIRE

    Abudabos, AM; Aljumaah, RS; Algawaan, AS; Al-Sornokh, H; Al-Atiyat, RM

    2017-01-01

    ABSTRACT In total, 806 eggs of free-range Hassawi indigenous chickens were collected from local farm in Saudi Arabia. Eggs were weekly collected for 11 weeks. Initial egg weight (IEW) was recorded, and eggs were graded into four classes (A: 35-40 g, B: 40-45 g, C: 45-50 g, and D: 50-55 g). Eggs were stored for seven days at 75-80% relative humidity and 14-16 C, after which egg weight losses (WL0) were calculated. During incubation, eggs were weighed on days 7 (W7) and 14 (W14), and egg weight...

  8. Food crystallization and eggs.

    Science.gov (United States)

    Egg products can be utilized to control crystallization in a diverse realm of food products. Albumen and egg yolk can aid in the control of sugar crystal formation in candies. Egg yolk can enhance the textural properties and aid in the control of large ice crystal formation in frozen desserts. In...

  9. Invasive Egg Predators and Food Availability Interactively Affect Maternal Investment in Egg Chemical Defense

    Directory of Open Access Journals (Sweden)

    Sarah C. Paul

    2018-01-01

    Full Text Available Invasive species commonly predate the offspring of native species and eggs are the life stage most vulnerable to this predation. In many species with no maternal care, females can alter the phenotype of eggs to protect them, for instance through chemical defense. In ladybirds egg alkaloids deter predators, including invasive predatory species of ladybirds, but conversely may attract cannibals who benefit from the consumption of eggs with higher alkaloid levels. Invasive predators tend to be more abundant where resources are also abundant, but in high resource environments the maternal fitness benefits of sibling cannibalism are low. Consequently this presents a conflict for female ladybirds between the different factors that influence egg alkaloid level, as protecting her eggs from predators might come with the cost of inadvertently encouraging within-clutch cannibalism under circumstances where it is not beneficial. We investigated how the ladybird Adalia bipunctata addresses this trade-off experimentally, by measuring the quantity of alkaloids in eggs laid by ladybirds in environments that differed in levels of resource availability and perceived predation risk from an invasive predator Harmonia axyridis. Females did lay eggs with higher egg alkaloid levels under poor resource conditions, but only when predator cues were absent. The resulting negative correlation between egg number and egg alkaloid level under poor resource conditions indicates a trade-off between these two attributes of maternal investment, mediated by female response to offspring predation risk. This implies that selection pressures on mothers to adaptively adjust the risk of siblicide may outweigh the need to protect offspring from interspecific predation. Our results demonstrate that maternal effects are an important aspect of species' responses to invasive predators, and highlight the value of studying maternal effects in the context of the multifaceted environments in

  10. Investigation of In-Package Ozonation: The Effectiveness of Ozone to Inactive Salmonella enteritidis on Raw, Shell Eggs

    Directory of Open Access Journals (Sweden)

    Austin Donner

    2011-01-01

    Full Text Available Food production, handling, and distribution practices pose a constant threat to the quality and safety of food products. The objective of this research is to evaluate an innovative in-package ozonation process to reduce Salmonella enteritidis on raw, shell eggs. Previous research has shown that in-package ozonation eliminates contaminants from outside sources, reduces pathogens, and extends shelf life. In this study, raw, shell eggs were inoculated with Salmonella enteritidis and exposed to ozonation treatment. Microbial recoveries were then tested to determine bacterial reductions. Measurements included: relative humidity (34 percent at 5oC, surface temperatures (oC, ozone concentrations, bacterial reductions of Salmonella enteritidis, and quality assessment of eggs (Haugh Unit [HU], color, pH, and weight. After a 24-hour storage period, all treated samples indicated 3 log10 reductions on average (previous research has achieved up to 6log10. These results show effective in-package ozonation treatment reducing Salmonella enteritidis on raw, shell eggs without significant effect on measured egg quality over time. Benefits of in-package ozonation include no heating, low power requirements (less or equal to 50 Watts, short treatment time (seconds to minutes, and adaptability into existing processes. Given its ability to ensure the safety and longevity of food products, this technology has great potential for utilization in the food processing industry.

  11. Effects of dietary supplementation of resveratrol on performance, egg quality, yolk cholesterol and antioxidant enzyme activity of laying hens.

    Science.gov (United States)

    Feng, Z H; Gong, J G; Zhao, G X; Lin, X; Liu, Y C; Ma, K W

    2017-10-01

    1. This experiment was conducted to evaluate the effects of dietary supplementation of resveratrol on laying performance, egg quality, egg yolk cholesterol and antioxidant enzyme activities of laying hens. 2. A total of 360 Beijing PINK-1 laying hens (60 weeks old) were randomly distributed among five dietary treatments, each of which included 6 replicates of 12 hens. Dietary treatments were basal diet supplemented with 0 (control), 0.5, 1.0, 2.0 and 4.0 g/kg diet resveratrol. The study lasted for 9 weeks including 1 week of adaptation and 8 weeks of the main experimental period. 3. The results indicated that dietary resveratrol significantly improved feed conversion ratios during 5-8 weeks and 1-8 weeks of the trial. Increasing dietary concentrations of the resveratrol linearly improved Haugh unit and albumen height of eggs. 4. The content of total cholesterol (TC), total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C) in serum and cholesterol in yolk was significantly decreased by dietary resveratrol, and there were significant linear correlations between these indexes and resveratrol supplemental levels. 5. Dietary resveratrol supplementation significantly improved serum Glutathione peroxidase (GSH-Px) enzyme activity and decreased serum malondialdehyde (MDA) content in groups with 2.0 and 4.0 g/kg resveratrol as compared to the control, respectively. However, supplementation of resveratrol did not affect the activity of serum superoxide dismutase (SOD). 6. It is concluded that resveratrol supplementation has a positive effect on performance, lipid-related traits and antioxidant activity of laying hens.

  12. Irradiation of liquid egg, frozen egg, powdered egg, egg yolk and white of egg: reducing the population of Salmonella enteritidis and sensory aspects and physico-chemical;Irradiacao de ovo liquido, congelado e ovo, gema e clara em po: reducao da populacao de Salmonella enteritidis e aspectos sensoriais e fisico-quimicos

    Energy Technology Data Exchange (ETDEWEB)

    Froehlich, Angela

    2004-07-01

    Eggs and their products have been incriminated in foodborne disease outbreaks due to Salmonella enteritidis contamination. Irradiation is a food preservation technology that could be applied to minimize the problem. The aim of this study was to determine the effect of irradiation in liquid and frozen egg as well as in powdered egg, egg yolk and egg white spiked with Salmonella enteritidis. Spiked samples of liquid egg, egg white and egg yolk were exposed to 0,5; 1,0; 1,5; 2,0; 2,5; 3,0 kGy and spiked samples of frozen and powdered egg were exposed to 0,5; 1,0; 1,5; 2,0; 2,5; 3,0; 3,5 e 4,0 kGy. Raw odour, cooked odour and taste of non inoculated and irradiated samples and non irradiated samples of egg and egg products were analysed by a trained penal. Viscosity and lipid oxidation (malonaldehyde concentration) were also determined. Doses of 2,0; 3,0; 3,5; 3,0 e 3,5 kGy reduced in 5 log the population of S. Enteritidis in liquid and frozen egg, powdered egg yolk, egg white and egg, respectively, with moderate alterations in relation to non irradiated samples detected by the trained penal. Viscosity and lipid oxidation in the powdered products, however, showed more intense alterations. Therefore, irradiation can be considered a feasible process for liquid and frozen egg while when applied to powdered products it should be considered the type of food product to which they will be added due to alterations in viscosity. (author)

  13. 21 CFR 160.150 - Frozen egg whites.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen egg whites. 160.150 Section 160.150 Food... HUMAN CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.150 Frozen egg whites. (a) Frozen egg whites, frozen egg albumen is the food prepared by freezing...

  14. Baseline Assessment of 25-Hydroxyvitamin D Reference Material and Proficiency Testing/External Quality Assurance Material Commutability: A Vitamin D Standardization Program Study.

    Science.gov (United States)

    Phinney, Karen W; Sempos, Christopher T; Tai, Susan S-C; Camara, Johanna E; Wise, Stephen A; Eckfeldt, John H; Hoofnagle, Andrew N; Carter, Graham D; Jones, Julia; Myers, Gary L; Durazo-Arvizu, Ramon; Miller, W Greg; Bachmann, Lorin M; Young, Ian S; Pettit, Juanita; Caldwell, Grahame; Liu, Andrew; Brooks, Stephen P J; Sarafin, Kurtis; Thamm, Michael; Mensink, Gert B M; Busch, Markus; Rabenberg, Martina; Cashman, Kevin D; Kiely, Mairead; Galvin, Karen; Zhang, Joy Y; Kinsella, Michael; Oh, Kyungwon; Lee, Sun-Wha; Jung, Chae L; Cox, Lorna; Goldberg, Gail; Guberg, Kate; Meadows, Sarah; Prentice, Ann; Tian, Lu; Brannon, Patsy M; Lucas, Robyn M; Crump, Peter M; Cavalier, Etienne; Merkel, Joyce; Betz, Joseph M

    2017-09-01

    The Vitamin D Standardization Program (VDSP) coordinated a study in 2012 to assess the commutability of reference materials and proficiency testing/external quality assurance materials for total 25-hydroxyvitamin D [25(OH)D] in human serum, the primary indicator of vitamin D status. A set of 50 single-donor serum samples as well as 17 reference and proficiency testing/external quality assessment materials were analyzed by participating laboratories that used either immunoassay or LC-MS methods for total 25(OH)D. The commutability test materials included National Institute of Standards and Technology Standard Reference Material 972a Vitamin D Metabolites in Human Serum as well as materials from the College of American Pathologists and the Vitamin D External Quality Assessment Scheme. Study protocols and data analysis procedures were in accordance with Clinical and Laboratory Standards Institute guidelines. The majority of the test materials were found to be commutable with the methods used in this commutability study. These results provide guidance for laboratories needing to choose appropriate reference materials and select proficiency or external quality assessment programs and will serve as a foundation for additional VDSP studies.

  15. [Denaturation of egg antigens by cooking].

    Science.gov (United States)

    Watanabe, Hiroko; Akaboshi, Chie; Sekido, Haruko; Tanaka, Kouki; Tanaka, Kazuko; Shimojo, Naoki

    2012-01-01

    Changes in egg protein contents by cooking were measured with an ELISA kit using Tris-HCl buffer in model foods including cake, meatballs, pasta and pudding made with whole egg, egg-white and egg-yolk. The egg protein contents were lowest in the deep-fried model foods of cakes and meatballs. Ovalbumin (OVA) was undetectable (meatballs, suggesting that processing temperature and uniform heat-treatment affect the detection of egg protein. Furthermore, egg protein contents were below 6 µg/g in the pouched meatballs and pasta made with egg-yolk, and OVA and OVM were not detected by Western blotting analysis with human IgE from patients' serum. On the other hand, processed egg proteins were detected with an ELISA kit using a surfactant and reductant in the extract buffer.

  16. Effects of Hen Age and Egg Weight Class on the Hatchability of Free Range Indigenous Chicken Eggs

    Directory of Open Access Journals (Sweden)

    AM Abudabos

    Full Text Available ABSTRACT In total, 806 eggs of free-range Hassawi indigenous chickens were collected from local farm in Saudi Arabia. Eggs were weekly collected for 11 weeks. Initial egg weight (IEW was recorded, and eggs were graded into four classes (A: 35-40 g, B: 40-45 g, C: 45-50 g, and D: 50-55 g. Eggs were stored for seven days at 75-80% relative humidity and 14-16 C, after which egg weight losses (WL0 were calculated. During incubation, eggs were weighed on days 7 (W7 and 14 (W14, and egg weight losses on days 7 (WL7 and 14 (WL14, and total loss (WL0-14 were calculated. Hatchling weight (CW was measured. The proportion of CW relative to egg weight loss (WL on days0, 7 and 14 days of incubation (CW:WL0; CW:WL7 and CW:WL14, respectively, and break out analyses, fertility (F,total hatchability (HC and hatchability of fertile eggs (HF were also calculated. IEW decreased (p<0.05 with hen age. Stored egg weight (SEW were decreased as hen age increased (p<0.05. WL7, WL14 and WL0-14 showed significant differences (p<0.001 and increased up to first six-week of egg collection time. Hen age affected CW:WL before incubation, and on days 7 and 14 of incubation. Fertility (F was affected (p<0.05 in unpredicted way of increasing and decreasing by hen age. Egg weight class affected SEW, W7and W14 (p<0.001. Class D eggs were the highest weight. Class C eggs had highest HC. In summary, hatching eggs of Hassawi hens were affected by hen age and egg weight in randomly increase and decrease .

  17. 21 CFR 160.185 - Dried egg yolks.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Dried egg yolks. 160.185 Section 160.185 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.185 Dried egg yolks. (a) Dried egg yolks, dried yolks is the food prepared by drying egg yolks that conform to...

  18. 21 CFR 160.190 - Frozen egg yolks.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen egg yolks. 160.190 Section 160.190 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.190 Frozen egg yolks. (a) Frozen egg yolks, frozen yolks is the food prepared by freezing egg yolks that...

  19. Minimal requirements for quality controls in radiotherapy with external beams; Controlli di qualita' essenziali in radioterapia con fasci esterni

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-07-01

    Physical dosimetric guidelines have been developed by the Italian National Institute of Health study group on quality assurance in radiotherapy to define protocols for quality controls in external beam radiotherapy. While the document does not determine strict rules or firm recommendations, it suggests minimal requirements for quality controls necessary to guarantee an adequate degree of accuracy in external beam radiotherapy. [Italian] Il gruppo di studio Assicurazione di qualita' in radioterapia dell'Istituto Superiore di Sanita' presenta le linee guida per la stesura dei protocolli di controllo di qualita' essenziali necessari a garantire un adeguato livello di accuratezza del trattamento radiante e rappresenta pertanto una parte essenziale del contributo fisico-dosimetrico globale di assicurazione di qualita' in radioterapia con fasci esterni.

  20. Plant responses to insect egg deposition

    NARCIS (Netherlands)

    Hilker, M.; Fatouros, N.E.

    2015-01-01

    Plants can respond to insect egg deposition and thus resist attack by herbivorous insects from the beginning of the attack, egg deposition. We review ecological effects of plant responses to insect eggs and differentiate between egg-induced plant defenses that directly harm the eggs and indirect