Special Sensor Microwave Imager/Sounder (SSMIS) Temperature Data Record (TDR) in netCDF

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Special Sensor Microwave Imager/Sounder (SSMIS) is a series of passive microwave conically scanning imagers and sounders onboard the DMSP satellites beginning...

An FPGA-Based Adaptable 200 MHz Bandwidth Channel Sounder for Wireless Communication Channel Characterisation

Directory of Open Access Journals (Sweden)

David L. Ndzi

2011-01-01

Full Text Available This paper describes the development of a fast adaptable FPGA-based wideband channel sounder with signal bandwidths of up to 200 MHz and channel sampling rates up to 5.4 kHz. The application of FPGA allows the user to vary the number of real-time channel response averages, channel sampling interval, and duration of measurement. The waveform, bandwidth, and frequency resolution of the sounder can be adapted for any channel under investigation. The design approach and technology used has led to a reduction in size and weight by more than 60%. This makes the sounder ideal for mobile time-variant wireless communication channels studies. Averaging allows processing gains of up to 30 dB to be achieved for measurement in weak signal conditions. The technique applied also improves reliability, reduces power consumption, and has shifted sounder design complexity from hardware to software. Test results show that the sounder can detect very small-scale variations in channels.

Observations of atmospheric structure using an acoustic sounder

International Nuclear Information System (INIS)

Shaw, N.A.

1974-11-01

An acoustic sounder has been used to monitor the vertical temperature structure of the lowest 1.5 km of the atmosphere over the meteorological field site at Argonne National Laboratory since February 1972. Additional records were obtained near St. Louis, Mo., during the month of August. Sounder records obtained during cloudless days on which no major synoptic events occurred are separated into three characteristic phases. The first phase is the rise of the morning inversion associated with increasing solar heating of the surface after dawn. The second phase is the period of strong convective activity that usually exists between about 1100 and 1600 local time in summer and which typically destroys the inversion. The third phase includes the gradual regeneration of the low level inversion through radiation cooling of the lowest levels, followed by a period of persistence throughout the night until the first phase begins again after sunrise. Analysis of records obtained from a single acoustic sounder operating in the vertically-pointing, monostatic mode is subject to the usual ambiguity regarding the relative importance of advective effects and local changes with time. To provide a spatial sampling facility, a mobile acoustic sounding system was constructed during 1972. Details of the mobile antenna acoustic baffle or cuff are given in the Appendix. (19 figures, 1 table) (U.S.)

Mesoscale Phenomenon Revealed by an Acoustic Sounder

DEFF Research Database (Denmark)

Lundtang Petersen, Erik; Jensen, Niels Otto

1976-01-01

A particular phenomenon observed on an acoustic sounder record is analyzed, and is interpreted as being associated with the passing of a land breeze front. A simple physical explanation of the frontal movements is suggested. The actual existence of the land breeze is demonstrated by examination...

Seasonal and diel patterns in sedimentary flux of krill fecal pellets recorded by an echo sounder

KAUST Repository

Rø stad, Anders; Kaartvedt, Stein

2013-01-01

We used a moored upward-facing 200 kHz echo sounder to address sedimentation of fecal pellets (FPs) from dielly migrating Meganyctiphanes norvegica. The echo sounder was located on the bottom at 150 m depth in the Oslofjord, Norway, and was cabled

Deep convective cloud characterizations from both broadband imager and hyperspectral infrared sounder measurements

Science.gov (United States)

Ai, Yufei; Li, Jun; Shi, Wenjing; Schmit, Timothy J.; Cao, Changyong; Li, Wanbiao

2017-02-01

Deep convective storms have contributed to airplane accidents, making them a threat to aviation safety. The most common method to identify deep convective clouds (DCCs) is using the brightness temperature difference (BTD) between the atmospheric infrared (IR) window band and the water vapor (WV) absorption band. The effectiveness of the BTD method for DCC detection is highly related to the spectral resolution and signal-to-noise ratio (SNR) of the WV band. In order to understand the sensitivity of BTD to spectral resolution and SNR for DCC detection, a BTD to noise ratio method using the difference between the WV and IR window radiances is developed to assess the uncertainty of DCC identification for different instruments. We examined the case of AirAsia Flight QZ8501. The brightness temperatures (Tbs) over DCCs from this case are simulated for BTD sensitivity studies by a fast forward radiative transfer model with an opaque cloud assumption for both broadband imager (e.g., Multifunction Transport Satellite imager, MTSAT-2 imager) and hyperspectral IR sounder (e.g., Atmospheric Infrared Sounder) instruments; we also examined the relationship between the simulated Tb and the cloud top height. Results show that despite the coarser spatial resolution, BTDs measured by a hyperspectral IR sounder are much more sensitive to high cloud tops than broadband BTDs. As demonstrated in this study, a hyperspectral IR sounder can identify DCCs with better accuracy.

The Expected Impacts of NPOESS Microwave and Infrared Sounder Radiances on Operational Numerical Weather Prediction and Data Assimilation Systems

Science.gov (United States)

Swadley, S. D.; Baker, N.; Derber, J.; Collard, A.; Hilton, F.; Ruston, B.; Bell, W.; Candy, B.; Kleespies, T. J.

2009-12-01

The NPOESS atmospheric sounding functionality will be accomplished using two separate sensor suites, the combined infrared (IR) and microwave (MW) sensor suite (CrIMSS), and the Microwave Imager/Sounder (MIS) instrument. CrIMSS consists of the Cross Track Infrared Sounder (CrIS) and the cross track Advanced Technology Microwave Sounder (ATMS), and is scheduled to fly on the NPOESS Preparatory Project (NPP), and NPOESS operational flight units C1 and C3. The MIS is a conical scanning polarimetric imager and sounder patterned after the heritage WindSat, and DMSP Special Sensor Microwave Imagers and Sounders (SSMI and SSMIS), and is scheduled for flight units C2, C3 and C4. ATMS combines the current operational Advanced Microwave Sounding Unit (AMSU) and the Microwave Humidity Sounder (MHS), but with an additional channel in the 51.76 GHz oxygen absorption region and 3 additional channels in the 165.5 and 183 GHz water vapor absorption band. CrIS is a Fourier Transform Spectrometer and will provide 159 shortwave IR channels, 433 mid-range IR channels, and 713 longwave IR channels. The heritage sensors for CrIS are the NASA Advanced Infrared Sounder (AIRS) and the MetOp-A Infrared Atmospheric Sounding Interferometer (IASI). Both AIRS and IASI are high quality, high spectral resolution sounders which represent a significant improvement in the effective vertical resolution over previous IR sounders. This presentation will give an overview of preparations underway for day-1 monitoring of NPP/NPOESS radiances, and subsequent operational radiance assimilation. These preparations capitalize on experience gained during the pre-launch preparations, sensor calibration/validation and operational assimilation for the heritage sensors. One important step is to use pre-flight sensor channel specifications, noise estimates and knowledge of the antenna patterns, to generate and test proxy NPP/NPOESS sensor observations in existing assimilation systems. Other critical factors for

Seasonal and diel patterns in sedimentary flux of krill fecal pellets recorded by an echo sounder

KAUST Repository

Røstad, Anders

2013-11-01

We used a moored upward-facing 200 kHz echo sounder to address sedimentation of fecal pellets (FPs) from dielly migrating Meganyctiphanes norvegica. The echo sounder was located on the bottom at 150 m depth in the Oslofjord, Norway, and was cabled to shore for continuous measurements during winter and spring. Records of sinking pellets were for the first time observed with an echo sounder. Seasonal patterns of sedimentation of krill FPs were strongly correlated with data from continuous measurement of fluorescence, which illustrate the development of the spring bloom. Sedimenting particles were first observed as fluorescence values started to increase at the end of February and continued to increase until the bloom suddenly culminated at the end of March. This collapse of the bloom was detected on the echo sounder as a pulse of slowly sinking acoustic targets over a 2 d period. Prior to this event, there was a strong diel pattern in sedimentation, which correlated, with some time lag, with the diel migration of krill foraging at night near the surface. Pellet average sinking speeds ranged between 423 m d−1 and 804 m d−1, with a strong relation to pellet target strength, which is an acoustic proxy for size. This novel approach shows that echo sounders may be a valuable tool in studies of vertical pellet flux and, thereby, carbon flux, providing temporal resolution and direct observation of the sedimentation process, which are not obtained from standard methods.

P-sounder: an airborne P-band ice sounding radar

DEFF Research Database (Denmark)

Dall, Jørgen; Skou, Niels; Kusk, Anders

2007-01-01

is to test new ice sounding techniques, e.g. polarimetry, synthetic aperture processing, and coherent clutter suppression. A system analysis involving ice scattering models confirms that it is feasible to detect the bedrock through 4 km of ice and to detect deep ice layers. The ice sounder design features...

Evidence of Convective Redistribution of Carbon Monoxide in Aura Tropospheric Emission Sounder (TES) and Microwave Limb Sounder (MLS) Observations

Science.gov (United States)

Manyin, Michael; Douglass, Anne; Schoeberl, Mark

2010-01-01

Vertical convective transport is a key element of the tropospheric circulation. Convection lofts air from the boundary layer into the free troposphere, allowing surface emissions to travel much further, and altering the rate of chemical processes such as ozone production. This study uses satellite observations to focus on the convective transport of CO from the boundary layer to the mid and upper troposphere. Our hypothesis is that strong convection associated with high rain rate regions leads to a correlation between mid level and upper level CO amounts. We first test this hypothesis using the Global Modeling Initiative (GMI) chemistry and transport model. We find the correlation is robust and increases as the precipitation rate (the strength of convection) increases. We next examine three years of CO profiles from the Tropospheric Emission Sounder (TES) and Microwave Limb Sounder (MLS) instruments aboard EOS Aura. Rain rates are taken from the Tropical Rainfall Measuring Mission (TRMM) 3B-42 multi-satellite product. Again we find a correlation between mid-level and upper tropospheric CO, which increases with rain rate. Our result shows the critical importance of tropical convection in coupling vertical levels of the troposphere in the transport of trace gases. The effect is seen most clearly in strong convective regions such as the Inter-tropical Convergence Zone.

UARS Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AL V001

Data.gov (United States)

National Aeronautics and Space Administration — The Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AL data product consists of daily, 4 degree increment latitude-ordered vertical profiles of...

UARS Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AT V001

Data.gov (United States)

National Aeronautics and Space Administration — The Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AT data product consists of daily, 65.536 second interval time-ordered vertical profiles of...

Direction-of-Arrival Analysis of Airborne Ice Depth Sounder Data

DEFF Research Database (Denmark)

Nielsen, Ulrik; Yan, Jie-Bang; Gogineni, Sivaprasad

2017-01-01

In this paper, we analyze the direction-of arrival(DOA) of the ice-sheet data collected over Jakobshavn Glacier with the airborne Multichannel Radar Depth Sounder (MCRDS) during the 2006 field season. We extracted weak ice–bed echoes buried in signals scattered by the rough surface of the fast...

Total column ozone retrieval using INSAT-3D sounder in the tropics ...

Indian Academy of Sciences (India)

important for ozone estimation and lower instrument noise results in better ozone ... the Indian Space Research Organisation (ISRO) ... tivity of the sounder ozone band corresponding to .... NOAA Climate Monitoring and Diagnostics Labo-.

Implementing earth observation and advanced satellite based atmospheric sounders for water resource and climate modelling

DEFF Research Database (Denmark)

Boegh, E.; Dellwik, Ebba; Hahmann, Andrea N.

2010-01-01

This paper discusses preliminary remote sensing (MODIS) based hydrological modelling results for the Danish island Sjælland (7330 km2) in relation to project objectives and methodologies of a new research project “Implementing Earth observation and advanced satellite based atmospheric sounders....... For this purpose, a) internal catchment processes will be studied using a Distributed Temperature Sensing (DTS) system, b) Earth observations will be used to upscale from field to regional scales, and c) at the largest scale, satellite based atmospheric sounders and meso-scale climate modelling will be used...

Development of the Advanced Technology Microwave Sounder (ATMS) for NPOESS C1

Science.gov (United States)

Brann, C.; Kunkee, D.

2008-12-01

The National Polar-orbiting Operational Environmental Satellite System's Advanced Technology Microwave Sounder (ATMS) is planned for flight on the first NPOESS mission (C1) in 2013. The C1 ATMS will be the second instrument of the ATMS series and will provide along with the companion Cross-track Infrared Sounder (CrIS), atmospheric temperature and moisture profiles for NPOESS. The first flight of the ATMS is scheduled in 2010 on the NPOESS Preparatory Project (NPP) satellite, which is an early instrument risk reduction component of the NPOESS mission. This poster will focus on the development of the ATMS for C1 including aspects of the sensor calibration, antenna beam and RF characteristics and scanning. New design aspects of the C1 ATMS, required primarily by parts obsolescence, will also be addressed in this poster.

Sensor System Performance Evaluation and Benefits from the NPOESS Airborne Sounder Testbed-Interferometer (NAST-I)

Science.gov (United States)

Larar, A.; Zhou, D.; Smith, W.

2009-01-01

Advanced satellite sensors are tasked with improving global-scale measurements of the Earth's atmosphere, clouds, and surface to enable enhancements in weather prediction, climate monitoring, and environmental change detection. Validation of the entire measurement system is crucial to achieving this goal and thus maximizing research and operational utility of resultant data. Field campaigns employing satellite under-flights with well-calibrated FTS sensors aboard high-altitude aircraft are an essential part of this validation task. The National Polar-orbiting Operational Environmental Satellite System (NPOESS) Airborne Sounder Testbed-Interferometer (NAST-I) has been a fundamental contributor in this area by providing coincident high spectral/spatial resolution observations of infrared spectral radiances along with independently-retrieved geophysical products for comparison with like products from satellite sensors being validated. This paper focuses on some of the challenges associated with validating advanced atmospheric sounders and the benefits obtained from employing airborne interferometers such as the NAST-I. Select results from underflights of the Aqua Atmospheric InfraRed Sounder (AIRS) and the Infrared Atmospheric Sounding Interferometer (IASI) obtained during recent field campaigns will be presented.

ISIS Topside-Sounder Plasma-Wave Investigations as Guides to Desired Virtual Wave Observatory (VWO) Data Search Capabilities

Science.gov (United States)

Benson, Robert F.; Fung, Shing F.

2008-01-01

Many plasma-wave phenomena, observed by space-borne radio sounders, cannot be properly explained in terms of wave propagation in a cold plasma consisting of mobile electrons and infinitely massive positive ions. These phenomena include signals known as plasma resonances. The principal resonances at the harmonics of the electron cyclotron frequency, the plasma frequency, and the upper-hybrid frequency are well explained by the warm-plasma propagation of sounder-generated electrostatic waves, Other resonances have been attributed to sounder-stimulated plasma instability and non-linear effects, eigenmodes of cylindrical electromagnetic plasma oscillations, and plasma memory processes. Data from the topside sounders of the International Satellites for Ionospheric Studies (ISIS) program played a major role in these interpretations. A data transformation and preservation effort at the Goddard Space Flight Center has produced digital ISIS topside ionograms and a metadata search program that has enabled some recent discoveries pertaining to the physics of these plasma resonances. For example, data records were obtained that enabled the long-standing question (several decades) of the origin of the plasma resonance at the fundamental electron cyclotron frequency to be explained [Muldrew, Radio Sci., 2006]. These data-search capabilities, and the science enabled by them, will be presented as a guide to desired data search capabilities to be included in the Virtual Wave Observatory (VWO).

High Resolution Infrared Radiation Sounder (HIRS) for the Nimbus F Spacecraft

Science.gov (United States)

Koenig, E. W.

1975-01-01

Flown on Nimbus F in June 1975, the high resolution infrared radiation sounder (HIRS) scans with a geographical resolution of 23KM and samples radiance in seventeen selected spectral channels from visible (.7 micron) to far IR (15 micron). Vertical temperature profiles and atmospheric moisture content can be inferred from the output. System operation and test results are described.

An Assessment of Data from the Advanced Technology Microwave Sounder at the Met Office

Directory of Open Access Journals (Sweden)

Amy Doherty

2015-01-01

Full Text Available An appraisal of the Advanced Technology Microwave Sounder (ATMS for use in numerical weather prediction (NWP is presented, including an assessment of the data quality, the impact on Met Office global forecasts in preoperational trials, and a summary of performance over a period of 17 months operational use. After remapping, the noise performance (NEΔT of the tropospheric temperature sounding channels is evaluated to be approximately 0.1 K, comparing favourably with AMSU-A. However, the noise is not random, differences between observations and simulations based on short-range forecast fields show a spurious striping effect, due to 1/f noise in the receiver. The amplitude of this signal is several tenths of a Kelvin, potentially a concern for NWP applications. In preoperational tests, adding ATMS data to a full Met Office system already exploiting data from four microwave sounders improves southern hemisphere mean sea level pressure forecasts in the 2- to 5-day range by 1-2%. In operational use, where data from five other microwave sounders is assimilated, forecast impact is typically between −0.05 and −0.1 J/kg (3.4% of total mean impact per day over the period 1 April to 31 July 2013. This suggests benefits beyond redundancy, associated with reducing already small analysis errors.

Measuring tropospheric wind with microwave sounders

Science.gov (United States)

Lambrigtsen, B.; Su, H.; Turk, J.; Hristova-Veleva, S. M.; Dang, V. T.

2017-12-01

In its 2007 "Decadal Survey" of earth science missions for NASA the U.S. National Research Council recommended that a Doppler wind lidar be developed for a three-dimensional tropospheric winds mission ("3D-Winds"). The technology required for such a mission has not yet been developed, and it is expected that the next Decadal Survey, planned to be released by the end of 2017, will put additional emphasis on the still pressing need for wind measurements from space. The first Decadal Survey also called for a geostationary microwave sounder (GMS) on a Precipitation and All-weather Temperature and Humidity (PATH) mission, which could be used to measure wind from space. Such a sounder, the Geostationary Synthetic Thinned Aperture Radiometer (GeoSTAR), has been developed at the Jet Propulsion Laboratory (JPL). The PATH mission has not yet been funded by NASA, but a low-cost subset of PATH, GeoStorm has been proposed as a hosted payload on a commercial communications satellite. Both PATH and GeoStorm would obtain frequent (every 15 minutes of better) measurements of tropospheric water vapor profiles, and they can be used to derive atmospheric motion vector (AMV) wind profiles, even in the presence of clouds. Measurement of wind is particularly important in the tropics, where the atmosphere is largely not in thermal balance and wind estimates cannot generally be derived from temperature and pressure fields. We report on simulation studies of AMV wind vectors derived from a GMS and from a cluster of low-earth-orbiting (LEO) small satellites (e.g., CubeSats). The results of two separate simulation studies are very encouraging and show that a ±2 m/s wind speed precision is attainable, which would satisfy WMO requirements. A GMS observing system in particular, which can be implemented now, would enable significant progress in the study of atmospheric dynamics. Copyright 2017 California Institute of Technology. Government sponsorship acknowledged

Microwave Atmospheric Sounder on CubeSat

Science.gov (United States)

Padmanabhan, S.; Brown, S. E.; Kangaslahti, P.; Cofield, R.; Russell, D.; Stachnik, R. A.; Su, H.; Wu, L.; Tanelli, S.; Niamsuwan, N.

2014-12-01

To accurately predict how the distribution of extreme events may change in the future we need to understand the mechanisms that influence such events in our current climate. Our current observing system is not well-suited for observing extreme events globally due to the sparse sampling and in-homogeneity of ground-based in-situ observations and the infrequent revisit time of satellite observations. Observations of weather extremes, such as extreme precipitation events, temperature extremes, tropical and extra-tropical cyclones among others, with temporal resolution on the order of minutes and spatial resolution on the order of few kms (cost passive microwave sounding and imaging sensors on CubeSats that would work in concert with traditional flagship observational systems, such as those manifested on large environmental satellites (i.e. JPSS,WSF,GCOM-W), to monitor weather extremes. A 118/183 GHz sensor would enable observations of temperature and precipitation extremes over land and ocean as well as tropical and extra-tropical cyclones. This proposed project would enable low cost, compact radiometer instrumentation at 118 and 183 GHz that would fit in a 6U Cubesat with the objective of mass-producing this design to enable a suite of small satellites to image the key geophysical parameters needed to improve prediction of extreme weather events. We take advantage of past and current technology developments at JPL viz. HAMSR (High Altitude Microwave Scanning Radiometer), Advanced Component Technology (ACT'08) to enable low-mass, low-power high frequency airborne radiometers. In this paper, we will describe the design and implementation of the 118 GHz temperature sounder and 183 GHz humidity sounder on the 6U CubeSat. In addition, a summary of radiometer calibration and retrieval techniques of temperature and humidity will be discussed. The successful demonstration of this instrument on the 6U CubeSat would pave the way for the development of a constellation which

NOAA JPSS Advanced Technology Microwave Sounder (ATMS)-based Tropical Cyclone (TC) Products from NDE

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The JPSS Microwave Sounder-based Tropical Cyclone (TC) Products provide estimates of tropical cyclone maximum wind speed, minimum sea level pressure, radii of 34,...

Science Study For A Low Cost Upper Atmosphere Sounder (LOCUS)

Science.gov (United States)

Gerber, D.; Swinyard, B. M.; Ellison, B. N.; Siddans, R.; Kerridge, B. J.; Plane, J. M. C.; Feng, W.

2013-12-01

We present the findings of an initial science study to define the spectral bands for the proposed Mesosphere / Lower Thermosphere (MLT) sounder LOCUS. The LOCUS mission (Fig 1) uses disruptive technologies to make key MLT species detectable globally by satellite remote sensing for the first time. This presentation summarises the technological and scientific foundation on which the current 4-band Terahertz (THz) and sub- millimetre wave (SMW) instrument configuration was conceived.

Combining Passive Microwave Sounders with CYGNSS information for improved retrievals: Observations during Hurricane Harvey

Science.gov (United States)

Schreier, M. M.

2017-12-01

The launch of CYGNSS (Cyclone Global Navigation Satellite System) has added an interesting component to satellite observations: it can provide wind speeds in the tropical area with a high repetition rate. Passive microwave sounders that are overpassing the same region can benefit from this information, when it comes to the retrieval of temperature or water profiles: the uncertainty about wind speeds has a strong impact on emissivity and reflectivity calculations with respect to surface temperature. This has strong influences on the uncertainty of retrieval of temperature and water content, especially under extreme weather conditions. Adding CYGNSS information to the retrieval can help to reduce errors and provide a significantly better sounder retrieval. Based on observations during Hurricane Harvey, we want to show the impact of CYGNSS data on the retrieval of passive microwave sensors. We will show examples on the impact on the retrieval from polar orbiting instruments, like the Advanced Technology Microwave Sounder (ATMS) and AMSU-A/B on NOAA-18 and 19. In addition we will also show the impact on retrievals from HAMSR (High Altitude MMIC Sounding Radiometer), which was flying on the Global Hawk during the EPOCH campaign. We will compare the results with other observations and estimate the impact of additional CYGNSS information on the microwave retrieval, especially on the impact in error and uncertainty reduction. We think, that a synergetic use of these different data sources could significantly help to produce better assimilation products for forecast assimilation.

Space Plasma Slab Studies using a new 3D Embedded Reconfigurable MPSoC Sounder

Science.gov (United States)

Dekoulis, George

2016-07-01

This paper presents recent ionospheric slab thickness measurements using a new mobile digital sounder system. The datasets obtained have been compared to the results of existing sounders in operation. The data validity has been verified. The slab thickness data allow constant monitoring of the lower ionosphere revealing the dynamic trends of the physical processes being involved. The prototype offers a tremendous amount of hardware processing power and a previously unseen response time in servicing the input and output data interfaces. This has been enabled by incorporating the latest three-dimensional Ultrascale+ technologies available commercially from the reconfigurable Field Programmable Gate Array (FPGA) computing industry. Furthermore, a previously developed Network-on-Chip (NoC) design methodology has been incorporated for connecting and controlling the application driven multiprocessor network. The system determines electron distributions, aggregate electromagnetic field gradients and plasma current density.

Broadband infrared beam splitter for spaceborne interferometric infrared sounder.

Science.gov (United States)

Yu, Tianyan; Liu, Dingquan; Qin, Yang

2014-10-01

A broadband infrared beam splitter (BS) on ZnSe substrate used for the spaceborne interferometric infrared sounder (SIIRS) is studied in the spectral range of 4.44-15 μm. Both broadband antireflection coating and broadband beam-splitter coating in this BS are designed and tested. To optimize the optical properties and the stability of the BS, suitable infrared materials were selected, and improved deposition techniques were applied. The designed structures matched experimental data well, and the properties of the BS met the application specification of SIIRS.

Variability at Multiple Scales: Using an Array of Current- and Pressure-Sensor Equipped Inverted Echo Sounders to Measure the Ocean

Science.gov (United States)

2016-11-29

of Current- and Pressure - Sensor Equipped Inverted Echo Sounders to Measure the Ocean 5b. GRANT NUMBER NOOO 14-15-1-2857 5c. PROGRAM ELEMENT NUMBER...inverted echo sounders (lESs) equipped with pressure and current sensors (CPIESs). CPIESs are moored instruments that measure the round-trip acoustic...at a range of spatial and temporal scales. The goals of this project were to enhance the pool of pressure - sensor equipped lESs available at the

Variability at Multiple Scales: Using an Array of Current and Pressure Sensor Equipped Inverted Echo Sounders to Measure the Ocean

Science.gov (United States)

2016-11-29

of Current- and Pressure - Sensor Equipped Inverted Echo Sounders to Measure the Ocean 5b. GRANT NUMBER NOOO 14-15-1-2857 5c. PROGRAM ELEMENT NUMBER...inverted echo sounders (lESs) equipped with pressure and current sensors (CPIESs). CPIESs are moored instruments that measure the round-trip acoustic...at a range of spatial and temporal scales. The goals of this project were to enhance the pool of pressure - sensor equipped lESs available at the

The Box Model and the Acoustic Sounder, a Case Study

DEFF Research Database (Denmark)

Jensen, Niels Otto; Lundtang Petersen, Erik

1979-01-01

Concentrations of SO2 in a large city during a subsidence situation are predicted as a function of time by means of a simple box model and the predictions are compared to actual SO2 concentration measurements. The agreement between model results and measurements is found to be excellent. The mode...... uses the height of the mixing layer as measured by means of an acoustic sounder. It is demonstrated that this height is a dominant factor in determining the variation of the SO2 concentration...

Assimilation of Feng-Yun-3B satellite microwave humidity sounder data over land

Science.gov (United States)

Chen, Keyi; Bormann, Niels; English, Stephen; Zhu, Jiang

2018-03-01

The ECMWF has been assimilating Feng-Yun-3B (FY-3B) satellite microwave humidity sounder (MWHS) data over ocean in an operational forecasting system since 24 September 2014. It is more difficult, however, to assimilate microwave observations over land and sea ice than over the open ocean due to higher uncertainties in land surface temperature, surface emissivity and less effective cloud screening. We compare approaches in which the emissivity is retrieved dynamically from MWHS channel 1 [150 GHz (vertical polarization)] with the use of an evolving emissivity atlas from 89 GHz observations from the MWHS onboard NOAA and EUMETSAT satellites. The assimilation of the additional data over land improves the fit of short-range forecasts to other observations, notably ATMS (Advanced Technology Microwave Sounder) humidity channels, and the forecast impacts are mainly neutral to slightly positive over the first five days. The forecast impacts are better in boreal summer and the Southern Hemisphere. These results suggest that the techniques tested allow for effective assimilation of MWHS/FY-3B data over land.

UARS Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AL V010 (UARIS3AL) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — The Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AL data product consists of daily, 4 degree increment latitude-ordered vertical profiles of...

Using multi-beam echo sounder backscatter data for sediment classification in very shallow water environments

NARCIS (Netherlands)

Amiri-Simkooei, A.R.; Snellen, M.; Simons, D.G.

2009-01-01

In a recent work described in Ref. [1], an angle-independent methodology was developed to use the multi-beam echo sounder backscatter (MBES) data for the seabed sediment classification. The method employs the backscatter data at a certain angle to obtain the number of sediment classes and to

The microwave limb sounder for the Upper Atmosphere Research Satellite

Science.gov (United States)

Waters, J. W.; Peckham, G. E.; Suttie, R. A.; Curtis, P. D.; Maddison, B. J.; Harwood, R. S.

1988-01-01

The Microwave Limb Sounder was designed to map the concentrations of trace gases from the stratosphere to the lower thermosphere, to improve understanding of the photochemical reactions which take place in this part of the atmosphere. The instrument will measure the intensity of thermal radiation from molecules in the atmosphere at frequencies corresponding to rotational absorption bands of chlorine monoxide, ozone, and water vapor. Molecular concentration profiles will be determined over a height range of 15 to 80 km (20 to 45 km for C10). The 57 deg inclination orbit proposed for the Upper Atmosphere Research Satellite will allow global coverage.

UARS Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AT V010 (UARIS3AT) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — The Improved Stratospheric and Mesospheric Sounder (ISAMS) Level 3AT data product consists of daily, 65.536 second interval time-ordered vertical profiles of...

Applications and Lessons Learned using Data from the Atmospheric Infrared Sounder

Science.gov (United States)

Ray, S. E.; Fetzer, E. J.; Olsen, E. T.; Lambrigtsen, B.; Pagano, T. S.; Teixeira, J.; Licata, S. J.; Hall, J. R.

2016-12-01

Applications and Lessons Learned using Data from the Atmospheric Infrared SounderSharon Ray, Jet Propulsion Laboratory, California Institute of Technology The Atmospheric Infrared Sounder (AIRS) on NASA's Aqua spacecraft has been returning daily global observations of Earth's atmospheric constituents and properties since 2002. With a 12-year data record and daily, global observations in near real-time, AIRS can play a role in applications that fall under many of the NASA Applied Sciences focus areas. AIRS' involvement in applications is two years in, so what have we learned and what are the pitfalls? AIRS has made gains in drought applications with products under consideration for inclusion in the U.S. Drought Monitor national map, as also with volcano rapid response with an internal alert system and automated products to help characterize plume extent. Efforts are underway with cold air aloft for aviation, influenza outbreak prediction, and vector borne disease. But challenges have occurred both in validation and in crossing the "valley of death" between products and decision makers. AIRS now has improved maps of standard products to be distributed in near real-time via NASA LANCE and by the Goddard DAAC as part of the Obama's administration Big Earth Data Initiative. In addition internal tools have been developed to support development and distribution of our application products. This talk will communicate the status of the AIRS applications effort along with lessons learned, and provide examples of new product imagery designed to best communicate AIRS data.

The JPSS CrIS Instrument and the Evolution of Space-Based Infrared Sounders

Science.gov (United States)

Glumb, Ronald; Suwinski, Lawrence; Wells, Steven; Glumb, Anna; Malloy, Rebecca; Colton, Marie

2018-01-01

This paper will summarize the development of infrared sounders since the 1970s, describe the technological hurdles that were overcome to provide ever-increasing performance capabilities, and highlight the radiometric performance of the CrIS instrument on JPSS-1 (CrIS-JPSS1). This includes details of the CrIS-JPSS1 measured noise-equivalent spectral radiance (NEdN) performance, radiometric uncertainty performance utilizing a new and improved internal calibration target, short-term and long-term repeatability, spectral uncertainty, and spectral stability. In addition, the full-resolution operating modes for CrIS-JPSS1 will be reviewed, including a discussion of how these modes will be used during on-orbit characterization tests. We will provide a brief update of CrIS-SNPP on-obit performance and the production status of the CrIS instruments for JPSS-2 through JPSS-4. Current technological challenges will also be reviewed, including how ongoing research and development is enabling improvements to future sounders. The expanding usage of infrared sounding data will also be discussed, including demonstration of value via data assimilation, the roles of the public/private sector in communicating the importance of sounding data for long-term observations, and the long road to success from research to operational data products.

Satellite Sounder Observations of Contrasting Tropospheric Moisture Transport Regimes: Saharan Air Layers, Hadley Cells, and Atmospheric Rivers

Energy Technology Data Exchange (ETDEWEB)

Nalli, Nicholas R.; Barnet, Christopher D.; Reale, Tony; Liu, Quanhua; Morris, Vernon R.; Spackman, J. Ryan; Joseph, Everette; Tan, Changyi; Sun, Bomin; Tilley, Frank; Leung, L. Ruby; Wolfe, Daniel

2016-12-01

This paper examines the performance of satellite sounder atmospheric vertical moisture proles (AVMP) under tropospheric conditions encompassing moisture contrasts driven by convection and advection transport mechanisms, specifically Atlantic Ocean Saharan air layers (SALs) and Pacific Ocean moisture conveyer belts (MCBs) commonly referred to as atmospheric rivers (ARs), both of these being mesoscale to synoptic meteorological phenomena within the vicinity of subtropical Hadley subsidence zones. Operational AVMP environmental data records retrieved from the Suomi National Polar-orbiting Partnership (SNPP) NOAA-Unique Combined Atmospheric Processing System (NUCAPS) are collocated with dedicated radiosonde observations (RAOBs) obtained from ocean-based intensive field campaigns; these RAOBs provide uniquely independent correlative truth data not assimilated into numerical weather prediction models for satellite sounder validation over open ocean. Using these marine-based data, we empirically assess the performance of the operational NUCAPS AVMP product for detecting and resolving these tropospheric moisture features over otherwise RAOB-sparse regions.

Interpreting Observations of Large-Scale Traveling Ionospheric Disturbances by Ionospheric Sounders

Science.gov (United States)

Pederick, L. H.; Cervera, M. A.; Harris, T. J.

2017-12-01

From July to October 2015, the Australian Defence Science and Technology Group conducted an experiment during which a vertical incidence sounder (VIS) was set up at Alice Springs Airport. During September 2015 this VIS observed the passage of many large-scale traveling ionospheric disturbances (TIDs). By plotting the measured virtual heights across multiple frequencies as a function of time, the passage of the TID can be clearly displayed. Using this plotting method, we show that all the TIDs observed during the campaign by the VIS at Alice Springs show an apparent downward phase progression of the crests and troughs. The passage of the TID can be more clearly interpreted by plotting the true height of iso-ionic contours across multiple plasma frequencies; the true heights can be obtained by inverting each ionogram to obtain an electron density profile. These plots can be used to measure the vertical phase speed of a TID and also reveal a time lag between events seen in true height compared to virtual height. To the best of our knowledge, this style of analysis has not previously been applied to other swept-frequency sounder observations. We develop a simple model to investigate the effect of the passage of a large-scale TID on a VIS. The model confirms that for a TID with a downward vertical phase progression, the crests and troughs will appear earlier in virtual height than in true height and will have a smaller apparent speed in true height than in virtual height.

Noise performance of microwave humidity sounders over their lifetime

Science.gov (United States)

Hans, Imke; Burgdorf, Martin; John, Viju O.; Mittaz, Jonathan; Buehler, Stefan A.

2017-12-01

The microwave humidity sounders Special Sensor Microwave Water Vapor Profiler (SSMT-2), Advanced Microwave Sounding Unit-B (AMSU-B) and Microwave Humidity Sounder (MHS) to date have been providing data records for 25 years. So far, the data records lack uncertainty information essential for constructing consistent long time data series. In this study, we assess the quality of the recorded data with respect to the uncertainty caused by noise. We calculate the noise on the raw calibration counts from the deep space views (DSVs) of the instrument and the noise equivalent differential temperature (NEΔT) as a measure for the radiometer sensitivity. For this purpose, we use the Allan deviation that is not biased from an underlying varying mean of the data and that has been suggested only recently for application in atmospheric remote sensing. Moreover, we use the bias function related to the Allan deviation to infer the underlying spectrum of the noise. As examples, we investigate the noise spectrum in flight for some instruments. For the assessment of the noise evolution in time, we provide a descriptive and graphical overview of the calculated NEΔT over the life span of each instrument and channel. This overview can serve as an easily accessible information for users interested in the noise performance of a specific instrument, channel and time. Within the time evolution of the noise, we identify periods of instrumental degradation, which manifest themselves in an increasing NEΔT, and periods of erratic behaviour, which show sudden increases of NEΔT interrupting the overall smooth evolution of the noise. From this assessment and subsequent exclusion of the aforementioned periods, we present a chart showing available data records with NEΔT processing to provide input values for the uncertainty propagation in the generation of a new set of Fundamental Climate Data Records (FCDRs) that are currently produced in the project Fidelity and Uncertainty in Climate data

Counter electrojet features in the Brazilian sector: simultaneous observation by radar, digital sounder and magnetometers

Directory of Open Access Journals (Sweden)

C. M. Denardini

2009-04-01

Full Text Available In the present work we show new results regarding equatorial counter electrojet (CEJ events in the Brazilian sector, based on the RESCO radar, two set of fluxgate magnetometer systems and a digital sounder. RESCO radar is a 50 MHz backscatter coherent radar installed in 1998 at São Luís (SLZ, 2.33° S, 44.60° W, an equatorial site. The Digital sounder routinely monitors the electron density profile at the radar site. The magnetometer systems are fluxgate-type installed at SLZ and Eusébio (EUS, 03.89° S, 38.44° W. From the difference between the horizontal component of magnetic field at SLZ station and the same component at EUS (EEJ ground strength several cases of westward morning electrojet and its normal inversion to the eastward equatorial electrojet (EEJ have been observed. Also, the EEJ ground strength has shown some cases of CEJ events, which been detected with the RESCO radar too. Detection of these events were investigated with respect to their time and height of occurrence, correlation with sporadic E (Es layers at the same time, and their spectral characteristics as well as the radar echo power intensity.

A New Inversion Routine to Produce Vertical Electron-Density Profiles from Ionospheric Topside-Sounder Data

Science.gov (United States)

Wang, Yongli; Benson, Robert F.

2011-01-01

Two software applications have been produced specifically for the analysis of some million digital topside ionograms produced by a recent analog-to-digital conversion effort of selected analog telemetry tapes from the Alouette-2, ISIS-1 and ISIS-2 satellites. One, TOPIST (TOPside Ionogram Scalar with True-height algorithm) from the University of Massachusetts Lowell, is designed for the automatic identification of the topside-ionogram ionospheric-reflection traces and their inversion into vertical electron-density profiles Ne(h). TOPIST also has the capability of manual intervention. The other application, from the Goddard Space Flight Center based on the FORTRAN code of John E. Jackson from the 1960s, is designed as an IDL-based interactive program for the scaling of selected digital topside-sounder ionograms. The Jackson code has also been modified, with some effort, so as to run on modern computers. This modification was motivated by the need to scale selected ionograms from the millions of Alouette/ISIS topside-sounder ionograms that only exist on 35-mm film. During this modification, it became evident that it would be more efficient to design a new code, based on the capabilities of present-day computers, than to continue to modify the old code. Such a new code has been produced and here we will describe its capabilities and compare Ne(h) profiles produced from it with those produced by the Jackson code. The concept of the new code is to assume an initial Ne(h) and derive a final Ne(h) through an iteration process that makes the resulting apparent-height profile fir the scaled values within a certain error range. The new code can be used on the X-, O-, and Z-mode traces. It does not assume any predefined profile shape between two contiguous points, like the exponential rule used in Jackson s program. Instead, Monotone Piecewise Cubic Interpolation is applied in the global profile to keep the monotone nature of the profile, which also ensures better smoothness

Mechanical Description of the Mars Climate Sounder Instrument

Science.gov (United States)

Jau, Bruno M.

2008-01-01

This paper introduces the Mars Climate Sounder (MCS) Instrument of the Mars Reconnaissance Orbiter (MRO) spacecraft. The instrument scans the Martian atmosphere almost continuously to systematically acquire weather and climate observations over time. Its primary components are an optical bench that houses dual telescopes with a total of nine channels for visible and infrared sensing, and a two axis gimbal that provides pointing capabilities. Both rotating joints consist of an integrated actuator with a hybrid planetary/harmonic transmission and a twist cap section that enables the electrical wiring to pass through the rotating joint. Micro stepping is used to reduce spacecraft disturbance torques to acceptable levels while driving the stepper motors. To ensure survivability over its four year life span, suitable mechanical components, lubrication, and an active temperature control system were incorporated. Some life test results and lessons learned are provided to serve as design guidelines for actuator parts and flex cables.

Plasma density measurements from the GEOS-1 relaxation sounder

International Nuclear Information System (INIS)

Etcheto, J.; Bloch, J.J.

1978-01-01

The relaxation sounder uses the characteristics of the propagation of radiowaves to sound the plasma surrounding the spacecraft. It determines, in particular, the plasma frequency, which gives the electron density. Measurements over the whole dayside of the magnetosphere, from the evening to the night sectors, are now available. The behaviour of the plasma resonance depends on local time, the nighttime echoes being generally weaker. Density measurements thus obtained are shown and discussed in the context of what is presently known about the plasma distribution in the magnetosphere. In particular, the density around apogee is studied as a function of magnetic activity. On the dayside, it appears to vary between a few and a few tens of electrons per cubic centimeter. The evolution of the density profile for several consecutive days is studied and interpreted tracing back the drift of the particles. (Auth.)

Automatic detection of subglacial lakes in radar sounder data acquired in Antarctica

Science.gov (United States)

Ilisei, Ana-Maria; Khodadadzadeh, Mahdi; Dalsasso, Emanuele; Bruzzone, Lorenzo

2017-10-01

Subglacial lakes decouple the ice sheet from the underlying bedrock, thus facilitating the sliding of the ice masses towards the borders of the continents, consequently raising the sea level. This motivated increasing attention in the detection of subglacial lakes. So far, about 70% of the total number of subglacial lakes in Antarctica have been detected by analysing radargrams acquired by radar sounder (RS) instruments. Although the amount of radargrams is expected to drastically increase, from both airborne and possible future Earth observation RS missions, currently the main approach to the detection of subglacial lakes in radargrams is by visual interpretation. This approach is subjective and extremely time consuming, thus difficult to apply to a large amount of radargrams. In order to address the limitations of the visual interpretation and to assist glaciologists in better understanding the relationship between the subglacial environment and the climate system, in this paper, we propose a technique for the automatic detection of subglacial lakes. The main contribution of the proposed technique is the extraction of features for discriminating between lake and non-lake basal interfaces. In particular, we propose the extraction of features that locally capture the topography of the basal interface, the shape and the correlation of the basal waveforms. Then, the extracted features are given as input to a supervised binary classifier based on Support Vector Machine to perform the automatic subglacial lake detection. The effectiveness of the proposed method is proven both quantitatively and qualitatively by applying it to a large dataset acquired in East Antarctica by the MultiChannel Coherent Radar Depth Sounder.

Impact of local and non-local sources of pollution on background US Ozone: synergy of a low-earth orbiting and geostationary sounder constellation

Science.gov (United States)

Bowman, K. W.; Lee, M.

2015-12-01

Dramatic changes in the global distribution of emissions over the last decade have fundamentally altered source-receptor pollution impacts. A new generation of low-earth orbiting (LEO) sounders complimented by geostationary sounders over North America, Europe, and Asia providing a unique opportunity to quantify the current and future trajectory of emissions and their impact on global pollution. We examine the potential of this constellation of air quality sounders to quantify the role of local and non-local sources of pollution on background ozone in the US. Based upon an adjoint sensitivity method, we quantify the role synoptic scale transport of non-US pollution on US background ozone over months representative of different source-receptor relationships. This analysis allows us distinguish emission trajectories from megacities, e.g. Beijing, or regions, e.g., western China, from natural trends on downwind ozone. We subsequently explore how a combination of LEO and GEO observations could help quantify the balance of local emissions against changes in distant sources . These results show how this unprecedented new international ozone observing system can monitor the changing structure of emissions and their impact on global pollution.

Performance of a 1-micron, 1-joule Coherent Launch Site Atmospheric Wind Sounder

Science.gov (United States)

Hawley, James G.; Targ, Russell; Bruner, Richard; Henderson, Sammy W.; Hale, Charles P.; Vetorino, Steven; Lee, R. W.; Harper, Scott; Khan, Tayyab

1992-01-01

The paper describes the design and performance of the Coherent Launch Site Atmospheric Wind Sounder (CLAWS), which is a test and demonstration program designed for monitoring winds with a solid-state lidar in real time for the launch site vehicle guidance and control application. Analyses were conducted to trade off CO2 (9.11- and 10.6-microns), Ho:YAG (2.09 microns), and Nd:YAG (1.06-micron) laser-based lidars. The measurements set a new altitude record (26 km) for coherent wind measurements in the stratosphere.

The Whisper Relaxation Sounder onboard Cluster: A Powerful Tool for Space Plasma Diagnosis around the Earth

International Nuclear Information System (INIS)

Trotignon, J.G.; Decreau, P.M.E.; Rauch, J.L.; LeGuirriec, E.; Canu, P.; Darrouzet, F.

2001-01-01

The WHISPER relaxation sounder that is onboard the four CLUSTER spacecraft has for main scientific objectives to monitor the natural waves in the 2 kHz - 80 kHz frequency range and, mostly, to determine the total plasma density from the solar wind down to the Earth's plasmasphere. To fulfil these objectives, the WHISPER uses the two long double sphere antennae of the Electric Field and Wave experiment as transmitting and receiving sensors. In its active working mode, the WHISPER works according to principles that have been worked out for topside sounding. A radio wave transmitter sends an almost monochromatic and short wave train. A few milliseconds after, a receiver listens to the surrounding plasma response. Strong and long lasting echoes are actually received whenever the transmitting frequencies coincide with characteristic plasma frequencies. Provided that these echoes, also called resonances, may be identified, the WHISPER relaxation sounder becomes a reliable and powerful tool for plasma diagnosis. When the transmitter is off, the WHISPER behaves like a passive receiver, allowing natural waves to be monitored. The paper aims mainly at the resonance identification process description and the WHISPER capabilities and performance highlighting. (author)

Whisper, a resonance sounder and wave analyser: Performances and perspectives for the Cluster mission

DEFF Research Database (Denmark)

Decreau, P.M.E.; Fergeau, P.; KrannoselsKikh, V.

1997-01-01

The WHISPER sounder on the Cluster spacecraft is primarily designed to provide an absolute measurement of the total plasma density within the range 0.2-80 cm(-3). This is achieved by means of a resonance sounding technique which has already proved successful in the regions to be explored. The wav...... in the electron foreshock and solar wind, to investigations about small-scale structures via density and high-frequency emission signatures, and to the analysis of the non-thermal continuum in the magnetosphere....

Carbon Monoxide Distribution over Peninsular Malaysia from the Atmospheric Infrared Sounder (AIRS)

Science.gov (United States)

Rajab, Jaso M.; MatJafri, M. Z.; Lim, H. S.; Abdullah, K.

2009-07-01

The Atmospheric Infrared Sounder (AIRS) onboard NASA's Aqua satellite. It daily coverage of ˜70% of the planet represents a significant evolutionary advance in satellite traces gas remote sensing. AIRS, the part of a large international investment to upgrade the operational meteorological satellite systems, is first of the new generation of meteorological advanced sounders for operational and research use, Providing New Insights into Weather and Climate for the 21st Century. Carbon monoxide (CO) is a ubiquitous, an indoor and outdoor air pollutant, is not a significant greenhouse gas as it absorbs little infrared radiation from the Earth. However, it does have an influence on oxidization in the atmosphere through interaction with hydroxyl radicals (OH), which also react with methane, halocarbons and tropospheric ozone. It produced by the incomplete combustion of fossil fuels and biomass burning, and that it has a role as a smog. The aim of this investigation is to study the (CO) carbon monoxide distribution over Peninsular Malaysia. The land use map of the Peninsular Malaysia was conducted by using CO total column amount, obtained from AIRS data, the map & data was processed and analyzed by using Photoshop & SigmaPlot 11.0 programs and compared for timing of various (day time) (28 August 2005 & 29 August 2007) for both direct comparison and the comparison using the same a priori profile, the CO concentrations in 28/8/2005 higher. The CO maps were generated using Kriging Interpolation technique. This interpolation technique produced high correlation coefficient, R2 and low root mean square error, RMS for CO. This study provided useful information for influence change of CO concentration on varies temperature.

NOAA Climate Data Record (CDR) of Intersatellite Calibrated Clear-Sky High Resolution Infrared Radiation Sounder (HIRS) Channel 12 Brightness Temperature Version 3

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The High-Resolution Infrared Radiation Sounder (HIRS) of intersatellite calibrated channel 12 brightness temperature (TB) product is a gridded global monthly time...

Tropical stratospheric water vapor measured by the microwave limb sounder (MLS)

Science.gov (United States)

Carr, E. S.; Harwood, R. S.; Mote, P. W.; Peckham, G. E.; Suttie, R. A.; Lahoz, W. A.; O'Neill, A.; Froidevaux, L.; Jarnot, R. F.; Read, W. G.

1995-01-01

The lower stratospheric variability of equatorial water vapor, measured by the Microwave Limb Sounder (MLS), follows an annual cycle modulated by the quasi-biennial oscillation. At levels higher in the stratosphere, water vapor measurements exhibit a semi-annual oscillatory signal with the largest amplitudes at 2.2 and 1hPa. Zonal-mean cross sections of MLS water vapor are consistent with previous satellite measurements from the limb infrared monitor of the stratosphere (LIMS) and the stratospheric Aerosol and Gas Experiment 2 (SAGE 2) instruments in that they show water vapor increasing upwards and the polewards from a well defined minimum in the tropics. The minimum values vary in height between the retrieved 46 and 22hPa pressure levels.

Use of INSAT-3D sounder and imager radiances in the 4D-VAR data assimilation system and its implications in the analyses and forecasts

Science.gov (United States)

Indira Rani, S.; Taylor, Ruth; George, John P.; Rajagopal, E. N.

2016-05-01

INSAT-3D, the first Indian geostationary satellite with sounding capability, provides valuable information over India and the surrounding oceanic regions which are pivotal to Numerical Weather Prediction. In collaboration with UK Met Office, NCMRWF developed the assimilation capability of INSAT-3D Clear Sky Brightness Temperature (CSBT), both from the sounder and imager, in the 4D-Var assimilation system being used at NCMRWF. Out of the 18 sounder channels, radiances from 9 channels are selected for assimilation depending on relevance of the information in each channel. The first three high peaking channels, the CO2 absorption channels and the three water vapor channels (channel no. 10, 11, and 12) are assimilated both over land and Ocean, whereas the window channels (channel no. 6, 7, and 8) are assimilated only over the Ocean. Measured satellite radiances are compared with that from short range forecasts to monitor the data quality. This is based on the assumption that the observed satellite radiances are free from calibration errors and the short range forecast provided by NWP model is free from systematic errors. Innovations (Observation - Forecast) before and after the bias correction are indicative of how well the bias correction works. Since the biases vary with air-masses, time, scan angle and also due to instrument degradation, an accurate bias correction algorithm for the assimilation of INSAT-3D sounder radiance is important. This paper discusses the bias correction methods and other quality controls used for the selected INSAT-3D sounder channels and the impact of bias corrected radiance in the data assimilation system particularly over India and surrounding oceanic regions.

Space Electron Density Gradient Studies using a 3D Embedded Reconfigurable Sounder and ESA/NASA CLUSTER Mission

Science.gov (United States)

Dekoulis, George

2016-07-01

This paper provides a direct comparison between data captured by a new embedded reconfigurable digital sounder, different ground-based ionospheric sounders spread around Europe and the ESA/NASA CLUSTER mission. The CLUSTER mission consists of four identical space probes flying in a formation that allows measurements of the electron density gradient in the local magnetic field. Both the ground-based and the spacecraft instrumentations assist in studying the motion, geometry and boundaries of the plasmasphere. The comparison results are in accordance to each other. Some slight deviations among the captured data were expected from the beginning of this investigation. These small discrepancies are reasonable and seriatim analyzed. The results of this research are significant, since the level of the plasma's ionization, which is related to the solar activity, dominates the propagation of electromagnetic waves through it. Similarly, unusually high solar activity presents serious hazards to orbiting satellites, spaceborne instrumentation, satellite communications and infrastructure located on the Earth's surface. Long-term collaborative study of the data is required to continue, in order to identify and determine the enhanced risk in advance. This would allow scientists to propose an immediate cure.

A global climatology of stratospheric gravity waves from Atmospheric Infrared Sounder observations

Science.gov (United States)

Hoffmann, Lars; Xue, Xianghui; Alexander, M. Joan

2014-05-01

We present the results of a new study that aims on the detection and classification of `hotspots' of stratospheric gravity waves on a global scale. The analysis is based on a nine-year record (2003 to 2011) of radiance measurements by the Atmospheric Infrared Sounder (AIRS) aboard NASA's Aqua satellite. We detect the presence of stratospheric gravity waves based on 4.3 micron brightness temperature variances. Our method is optimized for peak events, i.e., strong gravity wave events for which the local variance considerably exceeds background levels. We estimated the occurrence frequencies of these peak events for different seasons and time of day and used the results to find local maxima of gravity wave activity. In addition, we use AIRS radiances at 8.1 micron to simultaneously detect convective events, including deep convection in the tropics and mesoscale convective systems at mid latitudes. We classified the gravity waves according to their sources, based on seasonal occurrence frequencies for convection and by means of topographic data. Our study reproduces well-known hotspots of gravity waves, e.g., the mountain wave hotspots at the Andes and the Antarctic Peninsula or the convective hotspot during the thunderstorm season over the North American Great Plains. However, the high horizontal resolution of the AIRS observations also helped us to locate several smaller hotspots, which were partly unknown or poorly studied so far. Most of these smaller hotspots are found near orographic features like small mountain ranges, in coastal regions, in desert areas, or near isolated islands. This new study will help to select the most promising regions and seasons for future observational studies of gravity waves. Reference: Hoffmann, L., X. Xue, and M. J. Alexander, A global view of stratospheric gravity wave hotspots located with Atmospheric Infrared Sounder observations, J. Geophys. Res., 118, 416-434, doi:10.1029/2012JD018658, 2013.

Noise performance of microwave humidity sounders over their lifetime

Directory of Open Access Journals (Sweden)

I. Hans

2017-12-01

Full Text Available The microwave humidity sounders Special Sensor Microwave Water Vapor Profiler (SSMT-2, Advanced Microwave Sounding Unit-B (AMSU-B and Microwave Humidity Sounder (MHS to date have been providing data records for 25 years. So far, the data records lack uncertainty information essential for constructing consistent long time data series. In this study, we assess the quality of the recorded data with respect to the uncertainty caused by noise. We calculate the noise on the raw calibration counts from the deep space views (DSVs of the instrument and the noise equivalent differential temperature (NEΔT as a measure for the radiometer sensitivity. For this purpose, we use the Allan deviation that is not biased from an underlying varying mean of the data and that has been suggested only recently for application in atmospheric remote sensing. Moreover, we use the bias function related to the Allan deviation to infer the underlying spectrum of the noise. As examples, we investigate the noise spectrum in flight for some instruments. For the assessment of the noise evolution in time, we provide a descriptive and graphical overview of the calculated NEΔT over the life span of each instrument and channel. This overview can serve as an easily accessible information for users interested in the noise performance of a specific instrument, channel and time. Within the time evolution of the noise, we identify periods of instrumental degradation, which manifest themselves in an increasing NEΔT, and periods of erratic behaviour, which show sudden increases of NEΔT interrupting the overall smooth evolution of the noise. From this assessment and subsequent exclusion of the aforementioned periods, we present a chart showing available data records with NEΔT  <  1 K. Due to overlapping life spans of the instruments, these reduced data records still cover without gaps the time since 1994 and may therefore serve as a first step for constructing long time

Scanning Mechanism of the FY-3 Microwave Humidity Sounder

Science.gov (United States)

Schmid, Manfred; Jing, Li; Hehr, Christian

2010-01-01

Astrium GmbH Germany, developed the scanning equipment for the instrument package of the MicroWave Humidity Sounder (MWHS) flying on the FY-3 meteorological satellite (FY means Feng Yun, Wind and Cloud) in a sun-synchronized orbit of 850-km altitude and at an inclination of 98.8 . The scanning mechanism rotates at variable velocity comprising several acceleration / deceleration phases during each revolution. The Scanning Mechanism contains two output shafts, each rotating a parabolic offset Antenna Reflector. The mechanism is operated in closed loop by means of redundant control electronics. MWHS is a sounding radiometer for measurement of global atmospheric water vapour profiles. An Engineering Qualification Model was developed and qualified and a first Flight Model was launched early 2008. The system is now working for more than two years successful in orbit. A second Flight Model of the Antenna Scanning Mechanism and of its associated control electronics was built and delivered to the customer for application on the follow-on spacecraft that will be launched by the end of 2010.

Satellite Sounder Data Assimilation for Improving Alaska Region Weather Forecast

Science.gov (United States)

Zhu, Jiang; Stevens, E.; Zavodsky, B. T.; Zhang, X.; Heinrichs, T.; Broderson, D.

2014-01-01

Data assimilation has been demonstrated very useful in improving both global and regional numerical weather prediction. Alaska has very coarser surface observation sites. On the other hand, it gets much more satellite overpass than lower 48 states. How to utilize satellite data to improve numerical prediction is one of hot topics among weather forecast community in Alaska. The Geographic Information Network of Alaska (GINA) at University of Alaska is conducting study on satellite data assimilation for WRF model. AIRS/CRIS sounder profile data are used to assimilate the initial condition for the customized regional WRF model (GINA-WRF model). Normalized standard deviation, RMSE, and correlation statistic analysis methods are applied to analyze one case of 48 hours forecasts and one month of 24-hour forecasts in order to evaluate the improvement of regional numerical model from Data assimilation. The final goal of the research is to provide improved real-time short-time forecast for Alaska regions.

The Impact of Cross-track Infrared Sounder (CrIS) Cloud-Cleared Radiances on Hurricane Joaquin (2015) and Matthew (2016) Forecasts

Science.gov (United States)

Wang, Pei; Li, Jun; Li, Zhenglong; Lim, Agnes H. N.; Li, Jinlong; Schmit, Timothy J.; Goldberg, Mitchell D.

2017-12-01

Hyperspectral infrared (IR) sounders provide high vertical resolution atmospheric sounding information that can improve the forecast skill in numerical weather prediction. Commonly, only clear radiances are assimilated, because IR sounder observations are highly affected by clouds. A cloud-clearing (CC) technique, which removes the cloud effects from an IR cloudy field of view (FOV) and derives the cloud-cleared radiances (CCRs) or clear-sky equivalent radiances, can be an alternative yet effective way to take advantage of the thermodynamic information from cloudy skies in data assimilation. This study develops a Visible Infrared Imaging Radiometer Suite (VIIRS)-based CC method for deriving Cross-track Infrared Sounder (CrIS) CCRs under partially cloudy conditions. Due to the lack of absorption bands on VIIRS, two important quality control steps are implemented in the CC process. Validation using VIIRS clear radiances indicates that the CC method can effectively obtain the CrIS CCRs for FOVs with partial cloud cover. To compare the impacts from assimilation of CrIS original radiances and CCRs, three experiments are carried out on two storm cases, Hurricane Joaquin (2015) and Hurricane Matthew (2016), using Gridpoint Statistical Interpolation assimilation system and Weather Research and Forecasting-Advanced Research Version models. At the analysis time, more CrIS observations are assimilated when using CrIS CCRs than with CrIS original radiances. Comparing temperature, specific humidity, and U/V winds with radiosondes indicates that the data impacts are growing larger with longer time forecasts (beyond 72 h forecast). Hurricane track forecasts also show improvements from the assimilation of CrIS CCRs due to better weather system forecasts. The impacts of CCRs on intensity are basically neutral with mixed positive and negative results.

Sound velocity from inverted echo sounders (IES) in the western Pacific Ocean from 1992-08-26 to 1993-03-22 (NODC Accession 9300159)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains inverted echo sounder data collected from two stations in the western Pacific, TPW nominally @ 2S and 154E and TPE nominally @ 2S and 164E....

Toward a standard line for use in multibeam echo sounder calibration

Science.gov (United States)

Weber, Thomas C.; Rice, Glen; Smith, Michael

2018-06-01

A procedure is suggested in which a relative calibration for the intensity output of a multibeam echo sounder (MBES) can be performed. This procedure identifies a common survey line (i.e., a standard line), over which acoustic backscatter from the seafloor is collected with multiple MBES systems or by the same system multiple times. A location on the standard line which exhibits temporal stability in its seafloor backscatter response is used to bring the intensity output of the multiple MBES systems to a common reference. This relative calibration procedure has utility for MBES users wishing to generate an aggregate seafloor backscatter mosaic using multiple systems, revisiting an area to detect changes in substrate type, and comparing substrate types in the same general area but with different systems or different system settings. The calibration procedure is demonstrated using three different MBES systems over 3 different years in New Castle, NH, USA.

Impact of advanced technology microwave sounder data in the NCMRWF 4D-VAR data assimilation system

Science.gov (United States)

Rani, S. Indira; Srinivas, D.; Mallick, Swapan; George, John P.

2016-05-01

This study demonstrates the added benefits of assimilating the Advanced Technology Microwave Sounder (ATMS) radiances from the Suomi-NPP satellite in the NCMRWF Unified Model (NCUM). ATMS is a cross-track scanning microwave radiometer inherited the legacy of two very successful instrument namely, Advanced Microwave Sounding Unit-A (AMSU-A) and Microwave Humidity Sounder (MHS). ATMS has 22 channels: 11 temperature sounding channels around 50-60 GHz oxygen band and 6 moisture sounding channels around the 183GHz water vapour band in addition to 5 channels sensitive to the surface in clear conditions, or to water vapour, rain, and cloud when conditions are not clear (at 23, 31, 50, 51 and 89 GHz). Before operational assimilation of any new observation by NWP centres it is standard practice to assess data quality with respect to NWP model background (short-forecast) fields. Quality of all channels is estimated against the model background and the biases are computed and compared against that from the similar observations. The impact of the ATMS data on global analyses and forecasts is tested by adding the ATMS data in the NCUM Observation Processing system (OPS) and 4D-Var variational assimilation (VAR) system. This paper also discusses the pre-operational numerical experiments conducted to assess the impact of ATMS radiances in the NCUM assimilation system. It is noted that the performance of ATMS is stable and it contributes to the performance of the model, complimenting observations from other instruments.

Identification of Critical Design Points for the EAP of a Space-based Doppler Lidar Wind Sounder

Science.gov (United States)

Emmitt, G. D.; Wood, S. A.

1992-01-01

The feasibility of making tropospheric wind measurements with a space-based Doppler lidar was studied by a number of agencies over the past 10-15 years. Currently NASA has a plan to launch such an instrument, the Laser Atmospheric Wind Sounder (LAWS), within the next decade. The design of the LAWS continues to undergo a series of iterations common to most instruments targeted for a space platform. In general, the constraints of available platform power, weight allowance, and project funds continue to change. With these changes the performance and design specifications also must change.

Airborne Deployment and Calibration of Microwave Atmospheric Sounder on 6U CubeSat

Science.gov (United States)

Padmanabhan, S.; Brown, S. T.; Lim, B.; Kangaslahti, P.; Russell, D.; Stachnik, R. A.

2015-12-01

To accurately predict how the distribution of extreme events may change in the future we need to understand the mechanisms that influence such events in our current climate. Our current observing system is not well-suited for observing extreme events globally due to the sparse sampling and in-homogeneity of ground-based in-situ observations and the infrequent revisit time of satellite observations. Observations of weather extremes, such as extreme precipitation events, temperature extremes, tropical and extra-tropical cyclones among others, with temporal resolution on the order of minutes and spatial resolution on the order of few kms (cost passive microwave sounding and imaging sensors on CubeSats that would work in concert with traditional flagship observational systems, such as those manifested on large environmental satellites (i.e. JPSS,WSF,GCOM-W), to monitor weather extremes. A 118/183 GHz sensor would enable observations of temperature and precipitation extremes over land and ocean as well as tropical and extra-tropical cyclones. This proposed project would enable low cost, compact radiometer instrumentation at 118 and 183 GHz that would fit in a 6U Cubesat with the objective of mass-producing this design to enable a suite of small satellites to image the key geophysical parameters needed to improve prediction of extreme weather events. We take advantage of past and current technology developments at JPL viz. HAMSR (High Altitude Microwave Scanning Radiometer), Advanced Component Technology (ACT'08) to enable low-mass, low-power high frequency airborne radiometers. In this paper, we will describe the design and implementation of the 118 GHz temperature sounder and 183 GHz humidity sounder on the 6U CubeSat. In addition, we will discuss the maiden airborne deployment of the instrument during the Plain Elevated Convection at Night (PECAN) experiment. The successful demonstration of this instrument on the 6U CubeSat would pave the way for the development of a

MTG infrared sounder detection chain: first radiometric test results

Science.gov (United States)

Dumestier, D.; Pistone, F.; Dartois, T.; Blazquez, E.

2017-11-01

Europe's next fleet of geostationary meteorological satellites, MeteoSat Third Generation, will introduce new functions in addition to continuity of high-resolution meteorological data. The atmosphere Infrared Sounder (IRS), as high -end instrument, is part of this challenging program. IRS principle is a Fourier Transform Interferometer, which allows recomposing atmospheric spectrum after infrared photons detection. Transmission spectrums will be used to support numerical weather prediction. IRS instrument is able to offer full disk coverage in one hour, an on-ground resolution of 4 by 4 km, in two spectral bands (MWIR: 1600 to 2175cm-1 and LWIR: 700 to 1210cm-1) with a spectral resolution of 0.6cm-1. Among critical technologies and processes, IRS detection chain shall offer outstanding characteristics in terms of radiometric performance like Signal to Noise Ratio (SNR), dynamic range and linearity. Selected detectors are HgCdTe two-dimensions arrays, cooled at 55 Kelvins, hybridized on snapshot silicon read-out circuit at 160x160 format. Video electronics present 16 bits resolution, and the whole detection chain (Detectors and electronics) permits to reach SNR between 2 000 and 10 000 as requested by the application. Radiometric onground test results performed on design representative detection chains are presented and are confirming the challenging phase A design choices.

Thermal Band Atmospheric Correction Using Atmospheric Profiles Derived from Global Positioning System Radio Occultation and the Atmospheric Infrared Sounder

Science.gov (United States)

Pagnutti, Mary; Holekamp, Kara; Stewart, Randy; Vaughan, Ronald D.

2006-01-01

This Rapid Prototyping Capability study explores the potential to use atmospheric profiles derived from GPS (Global Positioning System) radio occultation measurements and by AIRS (Atmospheric Infrared Sounder) onboard the Aqua satellite to improve surface temperature retrieval from remotely sensed thermal imagery. This study demonstrates an example of a cross-cutting decision support technology whereby NASA data or models are shown to improve a wide number of observation systems or models. The ability to use one data source to improve others will be critical to the GEOSS (Global Earth Observation System of Systems) where a large number of potentially useful systems will require auxiliary datasets as input for decision support. Atmospheric correction of thermal imagery decouples TOA radiance and separates surface emission from atmospheric emission and absorption. Surface temperature can then be estimated from the surface emission with knowledge of its emissivity. Traditionally, radiosonde sounders or atmospheric models based on radiosonde sounders, such as the NOAA (National Oceanic & Atmospheric Administration) ARL (Air Resources Laboratory) READY (Real-time Environmental Application and Display sYstem), provide the atmospheric profiles required to perform atmospheric correction. Unfortunately, these types of data are too spatially sparse and too infrequently taken. The advent of high accuracy, global coverage, atmospheric data using GPS radio occultation and AIRS may provide a new avenue for filling data input gaps. In this study, AIRS and GPS radio occultation derived atmospheric profiles from the German Aerospace Center CHAMP (CHAllenging Minisatellite Payload), the Argentinean Commission on Space Activities SAC-C (Satellite de Aplicaciones Cientificas-C), and the pair of NASA GRACE (Gravity Recovery and Climate Experiment) satellites are used as input data in atmospheric radiative transport modeling based on the MODTRAN (MODerate resolution atmospheric

Phase Change Material for Temperature Control of Imager or Sounder on GOES Type Satellites in GEO

Science.gov (United States)

Choi, Michael K.

2014-01-01

This paper uses phase change material (PCM) in the scan cavity of an imager or sounder on satellites in geostationary orbit (GEO) to maintain the telescope temperature stable. When sunlight enters the scan aperture, solar heating causes the PCM to melt. When sunlight stops entering the scan aperture, the PCM releases the thermal energy stored to keep the components in the telescope warm. It has no moving parts or bimetallic springs. It reduces heater power required to make up the heat lost by radiation to space through the aperture. It is an attractive thermal control option to a radiator with a louver and a sunshade.

The 2003 edition of geisa: a spectroscopic database system for the second generation vertical sounders radiance simulation

Science.gov (United States)

Jacquinet-Husson, N.; Lmd Team

The GEISA (Gestion et Etude des Informations Spectroscopiques Atmosphériques: Management and Study of Atmospheric Spectroscopic Information) computer accessible database system, in its former 1997 and 2001 versions, has been updated in 2003 (GEISA-03). It is developed by the ARA (Atmospheric Radiation Analysis) group at LMD (Laboratoire de Météorologie Dynamique, France) since 1974. This early effort implemented the so-called `` line-by-line and layer-by-layer '' approach for forward radiative transfer modelling action. The GEISA 2003 system comprises three databases with their associated management softwares: a database of spectroscopic parameters required to describe adequately the individual spectral lines belonging to 42 molecules (96 isotopic species) and located in a spectral range from the microwave to the limit of the visible. The featured molecules are of interest in studies of the terrestrial as well as the other planetary atmospheres, especially those of the Giant Planets. a database of absorption cross-sections of molecules such as chlorofluorocarbons which exhibit unresolvable spectra. a database of refractive indices of basic atmospheric aerosol components. Illustrations will be given of GEISA-03, data archiving method, contents, management softwares and Web access facilities at: http://ara.lmd.polytechnique.fr The performance of instruments like AIRS (Atmospheric Infrared Sounder; http://www-airs.jpl.nasa.gov) in the USA, and IASI (Infrared Atmospheric Sounding Interferometer; http://smsc.cnes.fr/IASI/index.htm) in Europe, which have a better vertical resolution and accuracy, compared to the presently existing satellite infrared vertical sounders, is directly related to the quality of the spectroscopic parameters of the optically active gases, since these are essential input in the forward models used to simulate recorded radiance spectra. For these upcoming atmospheric sounders, the so-called GEISA/IASI sub-database system has been elaborated

The WHISPER Relaxation Sounder and the CLUSTER Active Archive

Science.gov (United States)

Trotignon, J. G.; Décréau, P. M. E.; Rauch, J. L.; Vallières, X.; Rochel, A.; Kougblénou, S.; Lointier, G.; Facskó, G.; Canu, P.; Darrouzet, F.; Masson, A.

The Waves of HIgh frequency and Sounder for Probing of Electron density by Relaxation (WHISPER) instrument is part of the Wave Experiment Consortium (WEC) of the CLUSTER mission. With the help of the long double sphere antennae of the Electric Field and Wave (EFW) instrument and the Digital Wave Processor (DWP), it delivers active (sounding) and natural (transmitter off) electric field spectra, respectively from 4 to 82 kHz, and from 2 to 80 kHz. These frequency ranges have been chosen to include the electron plasma frequency, which is closely related to the total electron density, in most of the regions encountered by the CLUSTER spacecraft. Presented here is an overview of the WHISPER data products available in the CLUSTER Active Archive (CAA). The instrument and its performance are first recalled. The way the WHISPER products are obtained is then described, with particular attention being paid to the density determination. Both sounding and natural measurements are commonly used in this process, which depends on the ambient plasma regime. This is illustrated using drawings similar to the Bryant plots commonly used in the CLUSTER master science plan. These give a clear overview of typical density values and the parts of the orbits where they are obtained. More information on the applied software or on the quality/reliability of the density determination can also be highlighted.

Characteristics of monsoon inversions over the Arabian Sea observed by satellite sounder and reanalysis data sets

Directory of Open Access Journals (Sweden)

S. Dwivedi

2016-04-01

Full Text Available Monsoon inversion (MI over the Arabian Sea (AS is one of the important characteristics associated with the monsoon activity over Indian region during summer monsoon season. In the present study, we have used 5 years (2009–2013 of temperature and water vapour measurement data obtained from satellite sounder instrument, an Infrared Atmospheric Sounding Interferometer (IASI onboard MetOp satellite, in addition to ERA-Interim data, to study their characteristics. The lower atmospheric data over the AS have been examined first to identify the areas where MIs are predominant and occur with higher strength. Based on this information, a detailed study has been made to investigate their characteristics separately in the eastern AS (EAS and western AS (WAS to examine their contrasting features. The initiation and dissipation times of MIs, their percentage occurrence, strength, etc., has been examined using the huge database. The relation with monsoon activity (rainfall over Indian region during normal and poor monsoon years is also studied. WAS ΔT values are  ∼  2 K less than those over the EAS, ΔT being the temperature difference between 950 and 850 hPa. A much larger contrast between the WAS and EAS in ΔT is noticed in ERA-Interim data set vis-à-vis those observed by satellites. The possibility of detecting MI from another parameter, refractivity N, obtained directly from another satellite constellation of GPS Radio Occultation (RO (COSMIC, has also been examined. MI detected from IASI and Atmospheric Infrared Sounder (AIRS onboard the NOAA satellite have been compared to see how far the two data sets can be combined to study the MI characteristics. We suggest MI could also be included as one of the semipermanent features of southwest monsoon along with the presently accepted six parameters.

Atmospheric Sounder Spectrometer for Infrared Spectral Technology (ASSIST) Instrument Handbook

Energy Technology Data Exchange (ETDEWEB)

Flynn, Connor J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Atmospheric Radiation Measurement (ARM) Program

2016-03-01

The Atmospheric Sounder Spectrometer for Infrared Spectral Technology (ASSIST) measures the absolute infrared (IR) spectral radiance (watts per square meter per steradian per wavenumber) of the sky directly above the instrument. More information about the instrument can be found through the manufacturer’s website. The spectral measurement range of the instrument is 3300 to 520 wavenumbers (cm^-1) or 3-19.2 microns for the normal-range instruments and 3300 to 400 cm^-1 or 3-25 microns, for the extended-range polar instruments. Spectral resolution is 1.0 cm^-1. Instrument field-of-view is 1.3 degrees. Calibrated sky radiance spectra are produced on cycle of about 141 seconds with a group of 6 radiance spectra zenith having dwell times of about 14 seconds each interspersed with 55 seconds of calibration and mirror motion. The ASSIST data is comparable to the Atmospheric Emitted Radiance Interferometer (AERI) data and can be used for 1) evaluating line-by-line radiative transport codes, 2) detecting/quantifying cloud effects on ground-based measurements of infrared spectral radiance (and hence is valuable for cloud property retrievals), and 3) calculating vertical atmospheric profiles of temperature and water vapor and the detection of trace gases.

The Impact of Upper Tropospheric Humidity from Microwave Limb Sounder on the Midlatitude Greenhouse Effect

Science.gov (United States)

Hu, Hua; Liu, W. Timothy

1998-01-01

This paper presents an analysis of upper tropospheric humidity, as measured by the Microwave Limb Sounder, and the impact of the humidity on the greenhouse effect in the midlatitudes. Enhanced upper tropospheric humidity and an enhanced greenhouse effect occur over the storm tracks in the North Pacific and North Atlantic. In these areas, strong baroclinic activity and the large number of deep convective clouds transport more water vapor to the upper troposphere, and hence increase greenhouse trapping. The greenhouse effect increases with upper tropospheric humidity in areas with a moist upper troposphere (such as areas over storm tracks), but it is not sensitive to changes in upper tropospheric humidity in regions with a dry upper troposphere, clearly demonstrating that there are different mechanisms controlling the geographical distribution of the greenhouse effect in the midlatitudes.

Navigation Signal Disturbances by Multipath Propagation - Scaled Measurements with a Universal Channel Sounder Architecture

Science.gov (United States)

Geise, Robert; Neubauer, Bjoern; Zimmer, Georg

2015-11-01

The performance of navigation systems is always reduced by unwanted multipath propagation. This is especially of practical importance for airborne navigation systems like the instrument landing system (ILS) or the VHF omni directional radio range (VOR). Nevertheless, the quantitative analysis of corresponding, potentially harmful multipath propagation disturbances is very difficult due to the large parameter space. Experimentally difficulties arise due to very expensive, real scale measurement campaigns and numerical simulation techniques still have shortcomings which are briefly discussed. In this contribution a new universal approach is introduced on how to measure very flexibly multipath propagation effects for arbitrary navigation systems using a channel sounder architecture in a scaled measurement environment. Two relevant scenarios of multipath propagation and the impact on navigation signals are presented. The first describes disturbances of the ILS due to large taxiing aircraft. The other example shows the influence of rotating wind turbines on the VOR.

Acoustic-sounder investigation of the effects of boundary-layer decoupling on long-distance polutant transport

International Nuclear Information System (INIS)

Miller, E.L.

1976-01-01

The formation of the nocturnal surface temperature inversion results in a decrease in vertical momentum transfer which, in turn, is accompanied by an associated reduction in the transfer of pollutants from the atmosphere to surface sinks, thus decoupling the surface layer from the layer above the inversion. The diurnal oscillation in the surface temperature profiles may therefore have a significant effect upon the transport of atmospheric pollutants over long distances. Flights of a large manned balloon with a diverse array of chemical and meteorological instrumentation aboard, known as Project de Vinci, provided a unique opportunity to combine acoustic-sounder observations of qualitative temperature structure in the atmospheric boundary layer with the chemical measurements necessary to gain increased understanding of this decoupling process and its consequences for pollutant transport. The data collected on ozone on the balloon and the grounds are reported

Validation of UARS Microwave Limb Sounder 183 GHz H2O Measurements

Science.gov (United States)

Lahoz, W. A.; Suttie, M. R.; Froidevaux, L.; Harwood, R. S.; Lau, C. L.; Lungu, T. A.; Peckham, G. E.; Pumphrey, H. C.; Read, W. G.; Shippony, Z.;

Sensitivity Analysis for Atmospheric Infrared Sounder (AIRS) CO2 Retrieval

Science.gov (United States)

Gat, Ilana

2012-01-01

The Atmospheric Infrared Sounder (AIRS) is a thermal infrared sensor able to retrieve the daily atmospheric state globally for clear as well as partially cloudy field-of-views. The AIRS spectrometer has 2378 channels sensing from 15.4 micrometers to 3.7 micrometers, of which a small subset in the 15 micrometers region has been selected, to date, for CO2 retrieval. To improve upon the current retrieval method, we extended the retrieval calculations to include a prior estimate component and developed a channel ranking system to optimize the channels and number of channels used. The channel ranking system uses a mathematical formalism to rapidly process and assess the retrieval potential of large numbers of channels. Implementing this system, we identifed a larger optimized subset of AIRS channels that can decrease retrieval errors and minimize the overall sensitivity to other iridescent contributors, such as water vapor, ozone, and atmospheric temperature. This methodology selects channels globally by accounting for the latitudinal, longitudinal, and seasonal dependencies of the subset. The new methodology increases accuracy in AIRS CO2 as well as other retrievals and enables the extension of retrieved CO2 vertical profiles to altitudes ranging from the lower troposphere to upper stratosphere. The extended retrieval method for CO2 vertical profile estimation using a maximum-likelihood estimation method. We use model data to demonstrate the beneficial impact of the extended retrieval method using the new channel ranking system on CO2 retrieval.

Simulation study for the Stratospheric Inferred Wind (SIW) sub-millimeter limb sounder

Science.gov (United States)

Baron, Philippe; Murtagh, Donal; Eriksson, Patrick; Ochiai, Satoshi

2017-04-01

://www.ohb.de/press-releases-details/ohb-sweden-and-aac-microtec-to-develop-the-innosat-platform-and-implement-its-first-mission-named-mats.html [2] Wu D., et al.: Mesospheric Doppler wind measurements from Aura Microwave Limb Sounder (MLS), Advanced in Space Research, 42, 1246-1252, 2008 [3] Baron P., et al.: Observation of horizontal winds in the middle-atmosphere between 30S and 55N during the northern winter 2009-2010, Atmospheric Chemistry and Physics 13(13), 6049-6064, 2013, doi:10.5194/acp-13-6049-2013 [4] Baron P., et al.: Definition of an uncooled submillimeter/terahertz limb sounder for measuring middle atmospheric winds, Proceedings of ESA Living Planet Symposium, Edinburgh, UK, 9-13 September 2013, (ESA SP-722, December 2013)

Discrimination of fish layers using the three-dimensional information obtained by a split-beam echo-sounder

DEFF Research Database (Denmark)

Pedersen, Jens

1996-01-01

separation angle between neighbours around a reference fish was 68 degrees and 74 degrees, respectively. The estimated mean target strength (TS) was found to be significantly different for the two layers and conforms to the theoretical TS calculated from the diurnal species and size composition of the layers......This study attempts to illustrate the three-dimensional pattern of a ''pelagic'' and a ''benthic'' layer of fish using single- target information obtained using a split-beam echo-sounder. Parameters such as the nearest-neighbour distance and separation angle between the two nearest neighbours...... around a reference fish were used to discriminate between the two layers. The parameters estimated were found to be significantly different between the two layers. The mean nearest-neighbour distance estimated was 6.3 m and 5.8 m for the ''benthic'' and the ''pelagic'' layers, respectively, and the mean...

Preliminary Regional Analysis of the Kaguya Lunar Radar Sounder (LRS) Data through Eastern Mare Imbrium

Science.gov (United States)

Cooper, B.L.; Antonenko, I.; Yamaguchi, Y.; Osinski, G.; Ono, T.; Ku-mamoto, A.

2009-01-01

The Lunar Radar Sounder (LRS) experiment on board the Kaguya spacecraft is observing the subsurface structure of the Moon, using ground-penetrating radar operating in the frequency range of 5 MHz [1]. Because LRS data provides in-formation about lunar features below the surface, it allows us to improve our understanding of the processes that formed the Moon, and the post-formation changes that have occurred (such as basin formation and volcanism). We look at a swath of preliminary LRS data, that spans from 7 to 72 N, and from 2 to 10 W, passing through the eastern portion of Mare Imbrium (Figure 1). Using software, designed for the mineral exploration industry, we produce a preliminary, coarse 3D model, showing the regional structure beneath the study area. Future research will involve smaller subsets of the data in regions of interest, where finer structures, such as those identified in [2], can be studied.

Nonlinear bias analysis and correction of microwave temperature sounder observations for FY-3C meteorological satellite

Science.gov (United States)

Hu, Taiyang; Lv, Rongchuan; Jin, Xu; Li, Hao; Chen, Wenxin

2018-01-01

The nonlinear bias analysis and correction of receiving channels in Chinese FY-3C meteorological satellite Microwave Temperature Sounder (MWTS) is a key technology of data assimilation for satellite radiance data. The thermal-vacuum chamber calibration data acquired from the MWTS can be analyzed to evaluate the instrument performance, including radiometric temperature sensitivity, channel nonlinearity and calibration accuracy. Especially, the nonlinearity parameters due to imperfect square-law detectors will be calculated from calibration data and further used to correct the nonlinear bias contributions of microwave receiving channels. Based upon the operational principles and thermalvacuum chamber calibration procedures of MWTS, this paper mainly focuses on the nonlinear bias analysis and correction methods for improving the calibration accuracy of the important instrument onboard FY-3C meteorological satellite, from the perspective of theoretical and experimental studies. Furthermore, a series of original results are presented to demonstrate the feasibility and significance of the methods.

Stratigraphy and structural evolution of southern Mare Serenitatis - A reinterpretation based on Apollo Lunar Sounder Experiment data

Science.gov (United States)

Sharpton, V. L.; Head, J. W., III

1983-01-01

Two subsurface reflecting horizons have been detected by the Apollo Lunar Sounder Experiment (ALSE) in the southern Mare Serenitatis which appear to be regolith layers more than 2 m thick, and are correlated with major stratigraphic boundaries in the southeastern Mare Serenitatis. The present stratigraphic boundaries in the southeastern Mare Serenitatis. The present analysis implies that the lower horizon represents the interface between the earliest mare unit and the modified Serenitatis basin material below. The depth of volcanic fill within Serenitatis is highly variable, with an average thickness of mare basalts under the ALSE ground track of 1.6 km. Comparisons with the Orientale basin topography suggests that a major increaae in load thickness could occur a few km basinward of the innermost extent of the traverse. The history of volcanic infilling of Mare Serenitatis was characterized by three major episodes of volcanism.

The Transition of Atmospheric Infrared Sounder Total Ozone Products to Operations

Science.gov (United States)

Berndt, Emily; Zavodsky, Bradley; Jedlovec, Gary

2014-01-01

The National Aeronautics and Space Administration Short-term Prediction Research and Transition Center (NASA SPoRT) has transitioned a total column ozone product from the Atmospheric Infrared Sounder (AIRS) retrievals to the Weather Prediction Center and Ocean Prediction Center. The total column ozone product is used to diagnose regions of warm, dry, ozone-rich, stratospheric air capable of descending to the surface to create high-impact non-convective winds. Over the past year, forecasters have analyzed the Red, Green, Blue (RGB) Air Mass imagery in conjunction with the AIRS total column ozone to aid high wind forecasts. One of the limitations of the total ozone product is that it is difficult for forecasters to determine whether elevated ozone concentrations are related to stratospheric air or climatologically high values of ozone in certain regions. During the summer of 2013, SPoRT created an AIRS ozone anomaly product which calculates the percent of normal ozone based on a global stratospheric ozone mean climatology. With the knowledge that ozone values 125 percent of normal and greater typically represent stratospheric air; the anomaly product can be used with the total column ozone product to confirm regions of stratospheric air. This paper describes the generation of these products along with forecaster feedback concerning the use of the AIRS ozone products in conjunction with the RGB Air Mass product to access the utility and transition of the products.

Observation of the exhaust plume from the space shuttle main engines using the microwave limb sounder

Directory of Open Access Journals (Sweden)

H. C. Pumphrey

2011-01-01

Full Text Available A space shuttle launch deposits 700 tonnes of water in the atmosphere. Some of this water is released into the upper mesosphere and lower thermosphere where it may be directly detected by a limb sounding satellite instrument. We report measurements of water vapour plumes from shuttle launches made by the Microwave Limb Sounder (MLS on the Aura satellite. Approximately 50%–65% of shuttle launches are detected by MLS. The signal appears at a similar level across the upper 10 km of the MLS limb scan, suggesting that the bulk of the observed water is above the top of the scan. Only a small fraction at best of smaller launches (Ariane 5, Proton are detected. We conclude that the sensitivity of MLS is only just great enough to detect a shuttle sized launch, but that a suitably designed instrument of the same general type could detect the exhausts from a large proportion of heavy-lift launches.

THz limb sounder (TLS) for lower thermospheric wind, oxygen density, and temperature

Science.gov (United States)

Wu, Dong L.; Yee, Jeng-Hwa; Schlecht, Erich; Mehdi, Imran; Siles, Jose; Drouin, Brian J.

2016-07-01

Neutral winds are one of the most critical measurements in the lower thermosphere and E region ionosphere (LTEI) for understanding complex electrodynamic processes and ion-neutral interactions. We are developing a high-sensitivity, low-power, noncryogenic 2.06 THz Schottky receiver to measure wind profiles at 100-140 km. The new technique, THz limb sounder (TLS), aims to measure LTEI winds by resolving the wind-induced Doppler shift of 2.06 THz atomic oxygen (OI) emissions. As a transition between fine structure levels in the ground electronic state, the OI emission is in local thermodynamic equilibrium (LTE) at altitudes up to 350 km. This LTE property, together with day-and-night capability and small line-of-sight gradient, makes the OI limb sounding a very attractive technique for neutral wind observations. In addition to the wind measurement, TLS can also retrieve [OI] density and neutral temperature in the LTEI region. TLS leverages rapid advances in THz receiver technologies including subharmonically pumped (SHP) mixers and Schottky-diode-based power multipliers. Current SHP Schottky receivers have produced good sensitivity for THz frequencies at ambient environment temperatures (120-150 K), which are achievable through passively cooling in spaceflight. As an emerging technique, TLS can fill the critical data gaps in the LTEI neutral wind observations to enable detailed studies on the coupling and dynamo processes between charged and neutral molecules.

Upper tropospheric cloud systems determined from IR Sounders and their influence on the atmosphere

Science.gov (United States)

Stubenrauch, Claudia; Protopapadaki, Sofia; Feofilov, Artem; Velasco, Carola Barrientos

2017-02-01

Covering about 30% of the Earth, upper tropospheric clouds play a key role in the climate system by modulating the Earth's energy budget and heat transport. Infrared Sounders reliably identify cirrus down to an IR optical depth of 0.1. Recently LMD has built global cloud climate data records from AIRS and IASI observations, covering the periods from 2003-2015 and 2008-2015, respectively. Upper tropospheric clouds often form mesoscale systems. Their organization and properties are being studied by (1) distinguishing cloud regimes within 2° × 2° regions and (2) applying a spatial composite technique on adjacent cloud pressures, which estimates the horizontal extent of the mesoscale cloud systems. Convective core, cirrus anvil and thin cirrus of these systems are then distinguished by their emissivity. Compared to other studies of tropical mesoscale convective systems our data include also the thinner anvil parts, which make out about 30% of the area of tropical mesoscale convective systems. Once the horizontal and vertical structure of these upper tropospheric cloud systems is known, we can estimate their radiative effects in terms of top of atmosphere and surface radiative fluxes and by computing their heating rates.

Design and Implementation of a Mechanical Control System for the Scanning Microwave Limb Sounder

Science.gov (United States)

Bowden, William

2011-01-01

The Scanning Microwave Limb Sounder (SMLS) will use technological improvements in low noise mixers to provide precise data on the Earth's atmospheric composition with high spatial resolution. This project focuses on the design and implementation of a real time control system needed for airborne engineering tests of the SMLS. The system must coordinate the actuation of optical components using four motors with encoder readback, while collecting synchronized telemetric data from a GPS receiver and 3-axis gyrometric system. A graphical user interface for testing the control system was also designed using Python. Although the system could have been implemented with a FPGA-based setup, we chose to use a low cost processor development kit manufactured by XMOS. The XMOS architecture allows parallel execution of multiple tasks on separate threads-making it ideal for this application and is easily programmed using XC (a subset of C). The necessary communication interfaces were implemented in software, including Ethernet, with significant cost and time reduction compared to an FPGA-based approach. For these reasons, the XMOS technology is an attractive, cost effective, alternative to FPGA-based technologies for this design and similar rapid prototyping projects.

Performance of the HIRS/2 instrument on TIROS-N. [High Resolution Infrared Radiation Sounder

Science.gov (United States)

Koenig, E. W.

1980-01-01

The High Resolution Infrared Radiation Sounder (HIRS/2) was developed and flown on the TIROS-N satellite as one means of obtaining atmospheric vertical profile information. The HIRS/2 receives visible and infrared spectrum radiation through a single telescope and selects 20 narrow radiation channels by means of a rotating filter wheel. A passive radiant cooler provides an operating temperature of 106.7 K for the HgCdTe and InSb detectors while the visible detector operates at instrument frame temperature. Low noise amplifiers and digital processing provide 13 bit data for spacecraft data multiplexing and transmission. The qualities of system performance that determine sounding capability are the dynamic range of data collection, the noise equivalent radiance of the system, the registration of the air columns sampled in each channel and the ability to upgrade the calibration of the instrument to maintain the performance standard throughout life. The basic features, operating characteristics and performance of the instrument in test are described. Early orbital information from the TIROS-N launched on October 13, 1978 is given and some observations on system quality are made.

Space-Time Variations in Water Vapor as Observed by the UARS Microwave Limb Sounder

Science.gov (United States)

Elson, Lee S.; Read, William G.; Waters, Joe W.; Mote, Philip W.; Kinnersley, Jonathan S.; Harwood, Robert S.

1996-01-01

Water vapor in the upper troposphere has a significant impact on the climate system. Difficulties in making accurate global measurements have led to uncertainty in understanding water vapor's coupling to the hydrologic cycle in the lower troposphere and its role in radiative energy balance. The Microwave Limb Sounder (MLS) on the Upper Atmosphere Research Satellite is able to retrieve water vapor concentration in the upper troposphere with good sensitivity and nearly global coverage. An analysis of these preliminary retrievals based on 3 years of observations shows the water vapor distribution to be similar to that measured by other techniques and to model results. The primary MLS water vapor measurements were made in the stratosphere, where this species acts as a conserved tracer under certain conditions. As is the case for the upper troposphere, most of the stratospheric discussion focuses on the time evolution of the zonal mean and zonally varying water vapor. Stratospheric results span a 19-month period and tropospheric results a 36-month period, both beginning in October of 1991. Comparisons with stratospheric model calculations show general agreement, with some differences in the amplitude and phase of long-term variations. At certain times and places, the evolution of water vapor distributions in the lower stratosphere suggests the presence of meridional transport.

Improving Regional Forecast by Assimilating Atmospheric InfraRed Sounder (AIRS) Profiles into WRF Model

Science.gov (United States)

Chou, Shih-Hung; Zavodsky, Brad; Jedlovec, Gary J.

2009-01-01

In data sparse regions, remotely-sensed observations can be used to improve analyses and produce improved forecasts. One such source comes from the Atmospheric InfraRed Sounder (AIRS), which together with the Advanced Microwave Sounding Unit (AMSU), represents one of the most advanced space-based atmospheric sounding systems. The purpose of this paper is to describe a procedure to optimally assimilate high resolution AIRS profile data into a regional configuration of the Advanced Research WRF (ARW) version 2.2 using WRF-Var. The paper focuses on development of background error covariances for the regional domain and background type, and an optimal methodology for ingesting AIRS temperature and moisture profiles as separate overland and overwater retrievals with different error characteristics. The AIRS thermodynamic profiles are derived from the version 5.0 Earth Observing System (EOS) science team retrieval algorithm and contain information about the quality of each temperature layer. The quality indicators were used to select the highest quality temperature and moisture data for each profile location and pressure level. The analyses were then used to conduct a month-long series of regional forecasts over the continental U.S. The long-term impacts of AIRS profiles on forecast were assessed against verifying NAM analyses and stage IV precipitation data.

Mars @ ASDC

Science.gov (United States)

Carraro, Francesco

"Mars @ ASDC" is a project born with the goal of using the new web technologies to assist researches involved in the study of Mars. This project employs Mars map and javascript APIs provided by Google to visualize data acquired by space missions on the planet. So far, visualization of tracks acquired by MARSIS and regions observed by VIRTIS-Rosetta has been implemented. The main reason for the creation of this kind of tool is the difficulty in handling hundreds or thousands of acquisitions, like the ones from MARSIS, and the consequent difficulty in finding observations related to a particular region. This led to the development of a tool which allows to search for acquisitions either by defining the region of interest through a set of geometrical parameters or by manually selecting the region on the map through a few mouse clicks The system allows the visualization of tracks (acquired by MARSIS) or regions (acquired by VIRTIS-Rosetta) which intersect the user defined region. MARSIS tracks can be visualized both in Mercator and polar projections while the regions observed by VIRTIS can presently be visualized only in Mercator projection. The Mercator projection is the standard map provided by Google. The polar projections are provided by NASA and have been developed to be used in combination with APIs provided by Google The whole project has been developed following the "open source" philosophy: the client-side code which handles the functioning of the web page is written in javascript; the server-side code which executes the searches for tracks or regions is written in PHP and the DB which undergoes the system is MySQL.

Detection of heavy oil on the seabed by application of a 400 kHz multibeam echo sounder

International Nuclear Information System (INIS)

Wendelboe, G.; Fonseca, L.; Eriksen, M.; Mutschler, M.; Hvidbak, F.

2009-01-01

Marine spills of heavy oil that sink to the sea floor can have significant impacts on marine ecosystems. This paper described a program implemented by the United States Coast Guard to improve operational techniques for the detection, monitoring, and recovery of sunken oil. The program has developed an algorithm based on data from a multibeam echo sounder. The algorithm used calibrated backscatter strengths (BS) to produce a mosaic of the seabed. Values below a pre-specified threshold were sorted into groups using morphological filtering techniques. The angular response curves from each group were then analyzed and compared to a reference BS curve for heavy oil. Response curves below the upper bound curve were defined as oil. The algorithm had a 90 per cent accuracy rate at a recent demonstration using oil 6, Tesoro, Sundex, and asphalt samples. It was concluded that processing times per square mile are approximately 12 hours. Further studies will be conducted to reduce computation times by replacing raw beam-formed data with data that originated solely from the region near the seabed. 15 refs., 15 tabs., 18 figs

Verification of small-scale water vapor features in VAS imagery using high resolution MAMS imagery. [VISSR Atmospheric Sounder - Multispectral Atmospheric Mapping Sensor

Science.gov (United States)

Menzel, Paul W.; Jedlovec, Gary; Wilson, Gregory

1986-01-01

The Multispectral Atmospheric Mapping Sensor (MAMS), a modification of NASA's Airborne Thematic Mapper, is described, and radiances from the MAMS and the VISSR Atmospheric Sounder (VAS) are compared which were collected simultaneously on May 18, 1985. Thermal emission from the earth atmosphere system in eight visible and three infrared spectral bands (12.3, 11.2 and 6.5 microns) are measured by the MAMS at up to 50 m horizontal resolution, and the infrared bands are similar to three of the VAS infrared bands. Similar radiometric performance was found for the two systems, though the MAMS showed somewhat less attenuation from water vapor than VAS because its spectral bands are shifted to shorter wavelengths away from the absorption band center.

Investigating the Water Vapor Component of the Greenhouse Effect from the Atmospheric InfraRed Sounder (AIRS)

Science.gov (United States)

Gambacorta, A.; Barnet, C.; Sun, F.; Goldberg, M.

2009-12-01

We investigate the water vapor component of the greenhouse effect in the tropical region using data from the Atmospheric InfraRed Sounder (AIRS). Differently from previous studies who have relayed on the assumption of constant lapse rate and performed coarse layer or total column sensitivity analysis, we resort to AIRS high vertical resolution to measure the greenhouse effect sensitivity to water vapor along the vertical column. We employ a "partial radiative perturbation" methodology and discriminate between two different dynamic regimes, convective and non-convective. This analysis provides useful insights on the occurrence and strength of the water vapor greenhouse effect and its sensitivity to spatial variations of surface temperature. By comparison with the clear-sky computation conducted in previous works, we attempt to confine an estimate for the cloud contribution to the greenhouse effect. Our results compare well with the current literature, falling in the upper range of the existing global circulation model estimates. We value the results of this analysis as a useful reference to help discriminate among model simulations and improve our capability to make predictions about the future of our climate.

Dedicated Low Latitude Diurnal CO2 Frost Observation Campaigns by the Mars Climate Sounder

Science.gov (United States)

Piqueux, S.; Kass, D. M.; Kleinboehl, A.; Hayne, P. O.; Heavens, N. G.; McCleese, D. J.; Schofield, J. T.; Shirley, J. H.

2017-12-01

In December 2016 (Ls≈280, MY33) and July 2017 (Ls≈30, MY34), the Mars Climate Sounder (MCS) onboard the Mars Reconnaissance Orbiter (MRO) conducted two distinct observation campaigns. The first one aimed at 1) confirming the presence of low latitude diurnal CO2 frost on Mars, and 2) refining the estimated mass of carbon dioxide condensed at the surface, whereas the second campaign was designed to 3) search for temporally and spatially varying spectral characteristics indicative of frost properties (i.e., crystal size, contamination, etc.) and relationship to the regolith. To meet these goals, MCS acquired thermal infrared observations of the surface and atmosphere at variable local times (≈1.70-3.80 h Local True Solar Time) and in the 10°-50°N latitude band where very low thermal inertia material (frost distribution and spectral properties. In addition, pre-frost deposition surface cooling rates are found to be consistent with those predicted by numerical models (i.e., 1-2K per hour). Finally, we observe buffered surface temperatures near the local frost point, indicating a surface emissivity ≈1. (i.e., optically thin frost layers, or dust contaminated frost, or slab-like ice) and no discernable frost metamorphism. We will present a detailed analysis of these new and unique observations, and elaborate on the potential relationship between the regolith and this recurring frost cycle.

User expectations for multibeam echo sounders backscatter strength data-looking back into the future

Science.gov (United States)

Lucieer, Vanessa; Roche, Marc; Degrendele, Koen; Malik, Mashkoor; Dolan, Margaret; Lamarche, Geoffroy

2018-06-01

With the ability of multibeam echo sounders (MBES) to measure backscatter strength (BS) as a function of true angle of insonification across the seafloor, came a new recognition of the potential of backscatter measurements to remotely characterize the properties of the seafloor. Advances in transducer design, digital electronics, signal processing capabilities, navigation, and graphic display devices, have improved the resolution and particularly the dynamic range available to sonar and processing software manufacturers. Alongside these improvements the expectations of what the data can deliver has also grown. In this paper, we identify these user-expectations and explore how MBES backscatter is utilized by different communities involved in marine seabed research at present, and the aspirations that these communities have for the data in the future. The results presented here are based on a user survey conducted by the GeoHab (Marine Geological and Biological Habitat Mapping) association. This paper summarises the different processing procedures employed to extract useful information from MBES backscatter data and the various intentions for which the user community collect the data. We show how a range of backscatter output products are generated from the different processing procedures, and how these results are taken up by different scientific disciplines, and also identify common constraints in handling MBES BS data. Finally, we outline our expectations for the future of this unique and important data source for seafloor mapping and characterisation.

Thermal Stability of a 4 Meter Primary Reflector for the Scanning Microwave Limb Sounder

Science.gov (United States)

Cofield, Richard E.; Kasl, Eldon P.

2011-01-01

The Scanning Microwave Limb Sounder (SMLS) is a space-borne heterodyne radiometer which will measure pressure, temperature and atmospheric constituents from thermal emission in [180,680] GHz. SMLS, planned for the NRC Decadal Survey's Global Atmospheric Composition Mission, uses a novel toric Cassegrain antenna to perform both elevation and azimuth scanning. This provides better horizontal and temporal resolution and coverage than were possible with elevation-only scanning in the two previous MLS satellite instruments. SMLS is diffraction-limited in the vertical plane but highly astigmatic in the horizontal (beam aspect ratio approx. 1:20). Nadir symmetry ensures that beam shape is nearly invariant over plus or minus 65 deg azimuth. A low-noise receiver FOV is swept over the reflector system by a small azimuth-scanning mirror. We describe the fabrication and thermal-stability test of a composite demonstration primary reflector, having full 4m height and 1/3 the width planned for flight. Using finite-element models of reflectors and structure, we evaluate thermal deformations and optical performance for 4 orbital environments and isothermal soak. We compare deformations with photogrammetric measurements made during soak tests in a chamber. The test temperature range exceeds predicted orbital ranges by large factors, implying in-orbit thermal stability of 0.21 micron rms (root mean square)/C, which meets SMLS requirements.

Satellite Atmospheric Sounder IRFS-2 1. Analysis of Outgoing Radiation Spectra Measurements

Science.gov (United States)

Polyakov, A. V.; Timofeyev, Yu. M.; Virolainen, Ya. A.; Uspensky, A. B.; Zavelevich, F. S.; Golovin, Yu. M.; Kozlov, D. A.; Rublev, A. N.; Kukharsky, A. V.

2017-12-01

The outgoing radiation spectra measured by the IRFS-2 spectrometer onboard Meteor-M no. 2 satellite have been analyzed. Some statistical parameters of more than 106 spectra measured in spring in 2015 have been calculated. The radiation brightness temperature varied from ˜300 K (surface temperature) up to ˜210 K (tropopause temperature). The quite high variability of the longwave measured radiation has been demonstrated. The signal-to-noise ratio distinctively decreases in the shortwave region (higher than 1300 cm-1). Intercomparisons of IR sounders IRFS-2 with IASI and CrIS spectra showed that the discrepancies in the average spectra and their variability do not exceed measurement errors in the spectral region 660-1300 cm-1. A comparison of specially chosen pairs of the simultaneously measured spectra showed that the differences between IRFS-2 and European instruments in the region of the 15-μm CO2 band and the transparency windows 8-12 μm are less than 1 mW/(m2 sr cm-1) and no more than the differences between the two IASI instruments (-A and -B). The differences between measured and simulated spectra are less than 1 mW/(m2 sr cm-1) in the mean part of CO2 band. However, starting from 720 cm-1, values appear that reach 2-4 mW/(m2 sr cm-1). This is caused by the absence of precise information about the surface temperature. Further investigations into the possible reasons for the observed disagreements are required in order to improve both the method of initial processing and the radiative model of the atmosphere.

Bottom pressure, vertical acoustic round-trip travel time, and near-bottom currents data collected by Current-and-Pressure-recording Inverted Echo Sounders (CPIES), as part of the Kuroshio Extension System Study (KESS), from 26 April 2004 to 25 June 2006 in the Kuroshio Extension east of Japan (NODC Accession 0073269)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains Current and Pressure recording Inverted Echo Sounder (CPIES) measurements collected during the Kuroshio Extension System Study (KESS) under...

Detection of Earth-rotation Doppler shift from Suomi National Polar-Orbiting Partnership Cross-Track Infrared Sounder.

Science.gov (United States)

Chen, Yong; Han, Yong; Weng, Fuzhong

2013-09-01

The Cross-Track Infrared Sounder (CrIS) on the Suomi National Polar-Orbiting Partnership Satellite is a Fourier transform spectrometer and provides a total of 1305 channels for sounding the atmosphere. Quantifying the CrIS spectral accuracy, which is directly related to radiometric accuracy, is crucial for improving its data assimilation in numerical weather prediction. In this study, a cross-correlation method is used for detecting the effect of Earth-rotation Doppler shift (ERDS) on CrIS observations. Based on a theoretical calculation, the ERDS can be as large as about 1.3 parts in 10(6) (ppm) near Earth's equator and at the satellite scan edge for a field of regard (FOR) of 1 or 30. The CrIS observations exhibit a relative Doppler shift as large as 2.6 ppm for a FOR pair of 1 and 30 near the equator. The variation of the ERDS with latitude and scan position detected from CrIS observations is similar to that derived theoretically, which indicates that the spectral stability of the CrIS instrument is very high. To accurately calibrate CrIS spectral accuracy, the ERDS effect should be removed. Since the ERDS is easily predictable, the Doppler shift is correctable in the CrIS spectra.

New isostatic mounting concept for a space born Three Mirror Anastigmat (TMA) on the Meteosat Third Generation Infrared Sounder Instrument (MTG-IRS)

Science.gov (United States)

Freudling, Maximilian; Klammer, Jesko; Lousberg, Gregory; Schumacher, Jean-Marc; Körner, Christian

2016-07-01

A novel isostatic mounting concept for a space born TMA of the Meteosat Third Generation Infrared Sounder is presented. The telescope is based on a light-weight all-aluminium design. The mounting concept accommodates the telescope onto a Carbon-Fiber-Reinforced Polymer (CRFP) structure. This design copes with the high CTE mismatch without introducing high stresses into the telescope structure. Furthermore a Line of Sight stability of a few microrads under geostationary orbit conditions is provided. The design operates with full performance at a temperature 20K below the temperature of the CFRP structure and 20K below the integration temperature. The mounting will sustain launch loads of 47g. This paper will provide the design of the Back Telescope Assembly (BTA) isostatic mounting and will summarise the consolidated technical baseline reached following a successful Preliminary Design Review (PDR).

Development and characterization of the superconducting integrated receiver channel of the TELIS atmospheric sounder

International Nuclear Information System (INIS)

De Lange, Gert; Boersma, Dick; Dercksen, Johannes; Ermakov, Andrey B; Golstein, Hans; Hoogeveen, Ruud W M; De Jong, Leo; Khudchenko, Andrey V; Kinev, Nickolay V; Kiselev, Oleg S; Van Kuik, Bart; De Lange, Arno; Van Rantwijk, Joris; Selig, Avri M; De Vries, Ed; Birk, Manfred; Dmitriev, Pavel; Filippenko, Lyudmila V; Sobolev, Alexander S; Torgashin, Mikhail Yu

2010-01-01

The balloon-borne instrument TELIS (TErahertz and submillimetre LImb Sounder) is a three-channel superconducting heterodyne spectrometer for atmospheric research use. It detects spectral emission lines of stratospheric trace gases that have their rotational transitions at THz frequencies. One of the channels is based on the superconducting integrated receiver (SIR) technology. We demonstrate for the first time the capabilities of the SIR technology for heterodyne spectroscopy in general, and atmospheric limb sounding in particular. We also show that the application of SIR technology is not limited to laboratory environments, but that it is well suited for remote operation under harsh environmental conditions. Within a SIR the main components needed for a superconducting heterodyne receiver such as a superconductor-insulator-superconductor (SIS) mixer with a quasi-optical antenna, a flux-flow oscillator (FFO) as the local oscillator, and a harmonic mixer to phase lock the FFO are integrated on a single chip. Light weight and low power consumption combined with broadband operation and nearly quantum limited sensitivity make the SIR a perfect candidate for use in future airborne and space-borne missions. The noise temperature of the SIR was measured to be as low as 120 K, with an intermediate frequency band of 4-8 GHz in double-sideband operation. The spectral resolution is well below 1 MHz, confirmed by our measurements. Remote control of the SIR under flight conditions has been demonstrated in a successful balloon flight in Kiruna, Sweden. The sensor and instrument design are presented, as well as the preliminary science results from the first flight.

Development and characterization of the superconducting integrated receiver channel of the TELIS atmospheric sounder

Energy Technology Data Exchange (ETDEWEB)

De Lange, Gert; Boersma, Dick; Dercksen, Johannes; Ermakov, Andrey B; Golstein, Hans; Hoogeveen, Ruud W M; De Jong, Leo; Khudchenko, Andrey V; Kinev, Nickolay V; Kiselev, Oleg S; Van Kuik, Bart; De Lange, Arno; Van Rantwijk, Joris; Selig, Avri M; De Vries, Ed [SRON Netherlands Institute for Space Research, PO Box 800, 9700 AV Groningen (Netherlands); Birk, Manfred [DLR German Aerospace Centre, Remote Sensing Technology Institute, D-82234 Wessling (Germany); Dmitriev, Pavel; Filippenko, Lyudmila V; Sobolev, Alexander S; Torgashin, Mikhail Yu, E-mail: G.de.Lange@sron.n, E-mail: valery@hitech.cplire.r [Kotel' nikov Institute of Radio Engineering and Electronics, Russian Academy of Science, 11/7 Mokhovaya Street, 125009, Moscow (Russian Federation)

2010-04-15

The balloon-borne instrument TELIS (TErahertz and submillimetre LImb Sounder) is a three-channel superconducting heterodyne spectrometer for atmospheric research use. It detects spectral emission lines of stratospheric trace gases that have their rotational transitions at THz frequencies. One of the channels is based on the superconducting integrated receiver (SIR) technology. We demonstrate for the first time the capabilities of the SIR technology for heterodyne spectroscopy in general, and atmospheric limb sounding in particular. We also show that the application of SIR technology is not limited to laboratory environments, but that it is well suited for remote operation under harsh environmental conditions. Within a SIR the main components needed for a superconducting heterodyne receiver such as a superconductor-insulator-superconductor (SIS) mixer with a quasi-optical antenna, a flux-flow oscillator (FFO) as the local oscillator, and a harmonic mixer to phase lock the FFO are integrated on a single chip. Light weight and low power consumption combined with broadband operation and nearly quantum limited sensitivity make the SIR a perfect candidate for use in future airborne and space-borne missions. The noise temperature of the SIR was measured to be as low as 120 K, with an intermediate frequency band of 4-8 GHz in double-sideband operation. The spectral resolution is well below 1 MHz, confirmed by our measurements. Remote control of the SIR under flight conditions has been demonstrated in a successful balloon flight in Kiruna, Sweden. The sensor and instrument design are presented, as well as the preliminary science results from the first flight.

Nazi supporters fail to march / Kira Savchenko

Index Scriptorium Estoniae

Savchenko, Kira

2010-01-01

1. juulil Riias toimunud natside marsil toimunut on pealt vaadanud ka rahvusvahelised ajakirjanikud. Läti president Valdis Dombrovskis, välisminister Aivis Ronis ja Iisraeli välisminister mõistsid marsi hukka

GeoMEx: Geographic Information System (GIS) Prototype for Mars Express Data

Science.gov (United States)

Manaud, N.; Frigeri, A.; Ivanov, A. B.

2013-09-01

As of today almost a decade of observational data have been returned by the multidisciplinary instruments on-board the ESA's Mars Express spacecraft. All data are archived into the ESA's Planetary Science Archive (PSA), which is the central repository for all ESA's Solar System missions [1]. Data users can perform advanced queries and retrieve data from the PSA using graphical and map-based search interfaces, or via direct FTP download [2]. However the PSA still offers limited geometrical search and visualisation capabilities that are essential for scientists to identify their data of interest. A former study has shown [3] that this limitation is mostly due to the fact that (1) only a subset of the instruments observations geometry information has been modeled and ingested into the PSA, and (2) that the access to that information from GIS software is impossible without going through a cumbersome and undocumented process. With the increasing number of Mars GIS data sets available to the community [4], GIS software have become invaluable tools for researchers to capture, manage, visualise, and analyse data from various sources. Although Mars Express surface imaging data are natural candidates for use in a GIS environment, other non-imaging instruments data (subsurface, atmosphere, plasma) integration is being investigated [5]. The objective of this work is to develop a GIS prototype that will integrate all the Mars Express instruments observations geometry information into a spatial database that can be accessed from external GIS software using standard WMS and WFS protocols. We will firstly focus on the integration of surface and subsurface instruments data (HRSC, OMEGA, MARSIS). In addition to the geometry information, base and context maps of Mars derived from surface mapping instruments data will also be ingested into the system. The system back-end architecture will be implemented using open-source GIS frameworks: PostgreSQL/PostGIS for the database, and Map

Upgrades to the Mars Initial Reference Ionosphere (MIRI) Model Due to Observations from MAVEN, MEX and MRO.

Science.gov (United States)

Narvaez, C.; Mendillo, M.; Trovato, J.

2017-12-01

A semi-empirical model of the maximum electron density (Nmax) of the martian ionosphere [MIRI-mark-1](1) was derived from an initial set radar observations by the MEX/MARSIS instrument. To extend the model to full electron density profiles, normalized shapes of Ne(h) from a theoretical model(2) were calibrated by MIRI's Nmax. Subsequent topside ionosphere observations from MAVEN indicated that topside shapes from MEX/MARSIS(3) offered improved morphology. The MEX topside shapes were then merged to the bottomside shapes from the theoretical model. Using a larger set of MEX/MARSIS observations (07/31/2005 - 05/24/2015), a new specification of Nmax as a function of solar zenith angle and solar flux is now used to calibrate the normalized Ne(h) profiles. The MIRI-mark-2 model includes the integral with height of Ne(h) to form total electron content (TEC) values. Validation of the MIRI TEC was accomplished using an independent set of TEC derived from the SHARAD(4) experiment on MRO. (1) M. Mendillo, A. Marusiak, P. Withers, D. Morgan and D. Gurnett, A New Semi-empirical Model of the Peak Electron Density of the Martian Ionosphere, Geophysical Research Letters, 40, 1-5, doi:10.1002/2013GL057631, 2013. (2) Mayyasi, M. and M. Mendillo (2015), Why the Viking descent probes found only one ionospheric layer at Mars, Geophys. Res. Lett., 42, 7359-7365, doi:10.1002/2015GL065575 (3) Němec, F., D. Morgan, D. Gurnett, and D. Andrews (2016), Empirical model of the Martian dayside ionosphere: Effects of crustal magnetic fields and solar ionizing flux at higher altitudes, J. Geophys. Res. Space Physics, 121, 1760-1771, doi:10.1002/2015/A022060.(4) Campbell, B., and T. Watters (2016), Phase compensation of MARSIS subsurface sounding and estimation of ionospheric properties: New insights from SHARAD results, J.Geophys. Res. Planets, 121, 180-193, doi:10.1002/2015JE004917.

Pravilnõi gorod reshajet problemõ tsivilizatsii zhutkim obrazom

Index Scriptorium Estoniae

2005-01-01

Hollandi arhitektide Neville Marsi ja Saskia Vendeli asutatud rahvusvaheline arhitektide grupp Dynamic City Foundation analüüsib ja loob pilti tulevikulinnast. Nende projektist "Beijing Boom Tower", mis modelleerib maketil fragmenti Pekingist 2020. aastal

Regional Precipitation Forecast with Atmospheric InfraRed Sounder (AIRS) Profile Assimilation

Science.gov (United States)

Chou, S.-H.; Zavodsky, B. T.; Jedloved, G. J.

2010-01-01

Advanced technology in hyperspectral sensors such as the Atmospheric InfraRed Sounder (AIRS; Aumann et al. 2003) on NASA's polar orbiting Aqua satellite retrieve higher vertical resolution thermodynamic profiles than their predecessors due to increased spectral resolution. Although these capabilities do not replace the robust vertical resolution provided by radiosondes, they can serve as a complement to radiosondes in both space and time. These retrieved soundings can have a significant impact on weather forecasts if properly assimilated into prediction models. Several recent studies have evaluated the performance of specific operational weather forecast models when AIRS data are included in the assimilation process. LeMarshall et al. (2006) concluded that AIRS radiances significantly improved 500 hPa anomaly correlations in medium-range forecasts of the Global Forecast System (GFS) model. McCarty et al. (2009) demonstrated similar forecast improvement in 0-48 hour forecasts in an offline version of the operational North American Mesoscale (NAM) model when AIRS radiances were assimilated at the regional scale. Reale et al. (2008) showed improvements to Northern Hemisphere 500 hPa height anomaly correlations in NASA's Goddard Earth Observing System Model, Version 5 (GEOS-5) global system with the inclusion of partly cloudy AIRS temperature profiles. Singh et al. (2008) assimilated AIRS temperature and moisture profiles into a regional modeling system for a study of a heavy rainfall event during the summer monsoon season in Mumbai, India. This paper describes an approach to assimilate AIRS temperature and moisture profiles into a regional configuration of the Advanced Research Weather Research and Forecasting (WRF-ARW) model using its three-dimensional variational (3DVAR) assimilation system (WRF-Var; Barker et al. 2004). Section 2 describes the AIRS instrument and how the quality indicators are used to intelligently select the highest-quality data for assimilation

Assimilation of Atmospheric InfraRed Sounder (AIRS) Profiles using WRF-Var

Science.gov (United States)

Zavodsky, Brad; Jedlovec, Gary J.; Lapenta, William

2008-01-01

The Weather Research and Forecasting (WRF) model contains a three-dimensional variational (3DVAR) assimilation system (WRF-Var), which allows a user to join data from multiple sources into one coherent analysis. WRF-Var combines observations with a background field traditionally generated using a previous model forecast through minimization of a cost function. In data sparse regions, remotely-sensed observations may be able to improve analyses and produce improved forecasts. One such source comes from the Atmospheric Infrared Sounder (AIRS), which together with the Advanced Microwave Sounding Unit (AMSU), represents one of the most advanced space-based atmospheric sounding systems. The combined AIRS/AMSU system provides radiance measurements used as input to a sophisticated retrieval scheme which has been shown to produce temperature profiles with an accuracy of 1 K over 1 km layers and humidity profiles with accuracy of 15% in 2 km layers in both clear and partly cloudy conditions. The retrieval algorithm also provides estimates of the accuracy of the retrieved values at each pressure level, allowing the user to select profiles based on the required error tolerances of the application. The purpose of this paper is to describe a procedure to optimally assimilate high-resolution AIRS profile data into a regional configuration of the Advanced Research WRF (ARW) version 2.2 using WRF-Var. The paper focuses on development of background error covariances for the regional domain and background field type using gen_be and an optimal methodology for ingesting AIRS temperature and moisture profiles as separate overland and overwater retrievals with different error characteristics in the WRF-Var. The AIRS thermodynamic profiles are obtained from the version 5.0 Earth Observing System (EOS) science team retrieval algorithm and contain information about the quality of each temperature layer. The quality indicators are used to select the highest quality temperature and moisture

Tuhast tõusnud Phoenix jõudis Marsile elu võimalikkust uurima / Liisi Poll

Index Scriptorium Estoniae

Poll, Liisi, 1980-

2008-01-01

Vt. ka Postimees : na russkom jazõke 27. mai, lk. 8. Marsile jõudnud NASA automaatjaam Phoenix maandus naaberplaneedi põhjapoolusele lähemale kui ükski inimese leiutatud masin kunagi varem. Lisa: Marsi uurimiste ajalugu

Marssi pommitatakse anduritega / Tiit Kändler

Index Scriptorium Estoniae

Kändler, Tiit, 1948-

1999-01-01

Skeem: Pomm otsib Marsilt vett. Veel sel nädalal põrutatakse Marsi pihta kaks väikest andurit, mis tungivad planeedi pinna sisse kiirusega 600 kilomeetrit tunnis. Deep Space 2 nimelised andurid toimetab kohale Mars Polar Lander

Space weather and HF propagation along different paths of the Russian chirp sounders network

Science.gov (United States)

Kurkin, V. I.; Litovkin, G. I.; Matyushonok, S. M.; Vertogradov, G. G.; Ivanov, V. A.; Poddelsky, I. N.; Rozanov, S. V.; Uryadov, V. P.

This paper presents experimental data obtained on long paths (from 2200 km to 5700 km range) of Russian frequency modulated continues wave (chirp) sounders network for the period from 1998 to 2003. Four transmitters (near Magadan, Khabarovsk, Irkutsk, Norilsk) and four receivers (near Irkutsk, Yoshkar-Ola, Nizhny Novgorod, Rostov-on-Don) were combined into single network to investigate a influence of geomagnetic storms and substorms on HF propagation in Asian region of Russia. In this region the geographic latitudes are in greatest excess of magnetic latitudes. As a consequence, elements of the large-scale structure, such as the main ionospheric trough, and the zone of auroral ionization, are produced in the ionosphere at the background of a low electron ionization. Coordinated experiments were carried out using 3-day Solar-Geophysical activity forecast presented by NOAA Space Environment Center in Internet. Sounding operations were conducted in the frequency band 4 -- 30 MHz on a round-the-clock basis at 15-min intervals. Oblique-incidence sounding (OIS) ionograms were recorded during 5-7 days every season for some years. The comparison between experimental data and simulation of OIS ionograms using International Reference Ionospheric model (IRI-2001) allowed to estimate the forecast of HF propagation errors both under quiet condition and during geomagnetic disturbances. Strong deviations from median values of maximum observed frequencies on mid-latitude paths in daytime present a real challenge to ionospheric forecast. Subauroral and mid-latitude chirp-sounding paths run, respectively, near the northward and southward walls of the main ionospheric trough. This make sit possible to study the dynamics of the trough's boundaries under different geophysical conditions and assess the influence of ionization gradients and small-scale turbulence on HF signal characteristics. The signals off-great circle propagation were registered over a wide frequency range and for

HF doppler sounder measurements of the ionospheric signatures of small scale ULF waves

Directory of Open Access Journals (Sweden)

L. J. Baddeley

2005-07-01

Full Text Available An HF Doppler sounder, DOPE (DOppler Pulsation Experiment with three azimuthally-separated propagation paths is used to provide the first statistical examination of small scale-sized, high m waves where a direct measurement of the azimuthal wavenumber m, is made in the ionosphere. The study presents 27 events, predominantly in the post-noon sector. The majority of events are Pc4 waves with azimuthal m numbers ranging from –100 to –200, representing some of the smallest scale waves ever observed in the ionosphere. 4 Pc5 waves are observed in the post-noon sector. The fact that measurements for the wave azimuthal m number and the wave angular frequency are available allows the drift-bounce resonance condition to be used to hypothesise potential particle populations which could drive the waves through either a drift or drift-bounce resonance interaction mechanism. These results are compared with the statistical study presented by Baddeley et al. (2004 which investigated the statistical likelihood of such driving particle populations occurring in the magnetospheric ring current. The combination of these two studies indicates that any wave which requires a possible drift resonance interaction with particles of energies >60 keV, is statistically unlikely to be generated by such a mechanism. The evidence presented in this paper therefore suggests that in the pre-noon sector the drift-bounce resonance mechanism is statistically more likely implying an anti-symmetric standing wave structure while in the post-noon sector both a drift or drift-bounce resonance interaction is statistically possible, indicating both symmetric and anti-symmetric standing mode structures. A case study is also presented investigating simultaneous observations of a ULF wave in ground magnetometer and DOPE data. The event is in the lower m range of the statistical study and displays giant pulsation (Pg characteristics.

Keywords

HF doppler sounder measurements of the ionospheric signatures of small scale ULF waves

Directory of Open Access Journals (Sweden)

L. J. Baddeley

2005-07-01

Full Text Available An HF Doppler sounder, DOPE (DOppler Pulsation Experiment with three azimuthally-separated propagation paths is used to provide the first statistical examination of small scale-sized, high m waves where a direct measurement of the azimuthal wavenumber m, is made in the ionosphere. The study presents 27 events, predominantly in the post-noon sector. The majority of events are Pc4 waves with azimuthal m numbers ranging from –100 to –200, representing some of the smallest scale waves ever observed in the ionosphere. 4 Pc5 waves are observed in the post-noon sector. The fact that measurements for the wave azimuthal m number and the wave angular frequency are available allows the drift-bounce resonance condition to be used to hypothesise potential particle populations which could drive the waves through either a drift or drift-bounce resonance interaction mechanism. These results are compared with the statistical study presented by Baddeley et al. (2004 which investigated the statistical likelihood of such driving particle populations occurring in the magnetospheric ring current. The combination of these two studies indicates that any wave which requires a possible drift resonance interaction with particles of energies >60 keV, is statistically unlikely to be generated by such a mechanism. The evidence presented in this paper therefore suggests that in the pre-noon sector the drift-bounce resonance mechanism is statistically more likely implying an anti-symmetric standing wave structure while in the post-noon sector both a drift or drift-bounce resonance interaction is statistically possible, indicating both symmetric and anti-symmetric standing mode structures. A case study is also presented investigating simultaneous observations of a ULF wave in ground magnetometer and DOPE data. The event is in the lower m range of the statistical study and displays giant pulsation (Pg characteristics. Keywords. Ionosphere (Ionosphere

Land Surface Temperature and Emissivity Separation from Cross-Track Infrared Sounder Data with Atmospheric Reanalysis Data and ISSTES Algorithm

Directory of Open Access Journals (Sweden)

Yu-Ze Zhang

2017-01-01

Full Text Available The Cross-track Infrared Sounder (CrIS is one of the most advanced hyperspectral instruments and has been used for various atmospheric applications such as atmospheric retrievals and weather forecast modeling. However, because of the specific design purpose of CrIS, little attention has been paid to retrieving land surface parameters from CrIS data. To take full advantage of the rich spectral information in CrIS data to improve the land surface retrievals, particularly the acquisition of a continuous Land Surface Emissivity (LSE spectrum, this paper attempts to simultaneously retrieve a continuous LSE spectrum and the Land Surface Temperature (LST from CrIS data with the atmospheric reanalysis data and the Iterative Spectrally Smooth Temperature and Emissivity Separation (ISSTES algorithm. The results show that the accuracy of the retrieved LSEs and LST is comparable with the current land products. The overall differences of the LST and LSE retrievals are approximately 1.3 K and 1.48%, respectively. However, the LSEs in our study can be provided as a continuum spectrum instead of the single-channel values in traditional products. The retrieved LST and LSEs now can be better used to further analyze the surface properties or improve the retrieval of atmospheric parameters.

Mechanical design and qualification of IR filter mounts and filter wheel of INSAT-3D sounder for low temperature

Science.gov (United States)

Vora, A. P.; Rami, J. B.; Hait, A. K.; Dewan, C. P.; Subrahmanyam, D.; Kirankumar, A. S.

2017-11-01

Next generation Indian Meteorological Satellite will carry Sounder instrument having subsystem of filter wheel measuring Ø260mm and carrying 18 filters arranged in three concentric rings. These filters made from Germanium, are used to separate spectral channels in IR band. Filter wheel is required to be cooled to 214K and rotated at 600 rpm. This Paper discusses the challenges faced in mechanical design of the filter wheel, mainly filter mount design to protect brittle germanium filters from failure under stresses due to very low temperature, compactness of the wheel and casings for improved thermal efficiency, survival under vibration loads and material selection to keep it lighter in weight. Properties of Titanium, Kovar, Invar and Aluminium materials are considered for design. The mount has been designed to accommodate both thermal and dynamic loadings without introducing significant aberrations into the optics or incurring permanent alignment shifts. Detailed finite element analysis of mounts was carried out for stress verification. Results of the qualification tests are discussed for given temperature range of 100K and vibration loads of 12g in Sine and 11.8grms in Random at mount level. Results of the filter wheel qualification as mounted in Electro Optics Module (EOM) are also presented.

Validation of Aura Microwave Limb Sounder stratospheric water vapor measurements by the NOAA frost point hygrometer.

Science.gov (United States)

Hurst, Dale F; Lambert, Alyn; Read, William G; Davis, Sean M; Rosenlof, Karen H; Hall, Emrys G; Jordan, Allen F; Oltmans, Samuel J

2014-02-16

Differences between stratospheric water vapor measurements by NOAA frost point hygrometers (FPHs) and the Aura Microwave Limb Sounder (MLS) are evaluated for the period August 2004 through December 2012 at Boulder, Colorado, Hilo, Hawaii, and Lauder, New Zealand. Two groups of MLS profiles coincident with the FPH soundings at each site are identified using unique sets of spatiotemporal criteria. Before evaluating the differences between coincident FPH and MLS profiles, each FPH profile is convolved with the MLS averaging kernels for eight pressure levels from 100 to 26 hPa (~16 to 25 km) to reduce its vertical resolution to that of the MLS water vapor retrievals. The mean FPH - MLS differences at every pressure level (100 to 26 hPa) are well within the combined measurement uncertainties of the two instruments. However, the mean differences at 100 and 83 hPa are statistically significant and negative, ranging from -0.46 ± 0.22 ppmv (-10.3 ± 4.8%) to -0.10 ± 0.05 ppmv (-2.2 ± 1.2%). Mean differences at the six pressure levels from 68 to 26 hPa are on average 0.8% (0.04 ppmv), and only a few are statistically significant. The FPH - MLS differences at each site are examined for temporal trends using weighted linear regression analyses. The vast majority of trends determined here are not statistically significant, and most are smaller than the minimum trends detectable in this analysis. Except at 100 and 83 hPa, the average agreement between MLS retrievals and FPH measurements of stratospheric water vapor is better than 1%.

Impacts of field of view configuration of Cross-track Infrared Sounder on clear-sky observations.

Science.gov (United States)

Wang, Likun; Chen, Yong; Han, Yong

2016-09-01

Hyperspectral infrared radiance measurements from satellite sensors contain valuable information on atmospheric temperature and humidity profiles and greenhouse gases, and therefore are directly assimilated into numerical weather prediction (NWP) models as inputs for weather forecasting. However, data assimilations in current operational NWP models still mainly rely on cloud-free observations due to the challenge of simulating cloud-contaminated radiances when using hyperspectral radiances. The limited spatial coverage of the 3×3 field of views (FOVs) in one field of regard (FOR) (i.e., spatial gap among FOVs) as well as relatively large footprint size (14 km) in current Cross-track Infrared Sounder (CrIS) instruments limits the amount of clear-sky observations. This study explores the potential impacts of future CrIS FOV configuration (including FOV size and spatial coverage) on the amount of clear-sky observations by simulation experiments. The radiance measurements and cloud mask products (VCM) from the Visible Infrared Imager Radiometer Suite (VIIRS) are used to simulate CrIS clear-sky observation under different FOV configurations. The results indicate that, given the same FOV coverage (e.g., 3×3), the percentage of clear-sky FOVs and the percentage of clear-sky FORs (that contain at least one clear-sky FOV) both increase as the FOV size decreases. In particular, if the CrIS FOV size were reduced from 14 km to 7 km, the percentage of clear-sky FOVs increases from 9.02% to 13.51% and the percentage of clear-sky FORs increases from 18.24% to 27.51%. Given the same FOV size but with increasing FOV coverage in each FOR, the clear-sky FOV observations increases proportionally with the increasing sampling FOVs. Both reducing FOV size and increasing FOV coverage can result in more clear-sky FORs, which benefit data utilization of NWP data assimilation.

Neutral wind and density perturbations in the thermosphere created by gravity waves observed by the TIDDBIT sounder

Science.gov (United States)

Vadas, Sharon L.; Crowley, Geoff

2017-06-01

In this paper, we study the 10 traveling ionospheric disturbances (TIDs) observed at zobs˜283 km by the TIDDBIT ionospheric sounder on 30 October 2007 at 0400-0700 UT near Wallops Island, USA. These TIDs propagated northwest/northward and were previously found to be secondary gravity waves (GWs) from tropical storm Noel. An instrumented sounding rocket simultaneously measured a large neutral wind peak uH' with a similar azimuth at z ˜ 325 km. Using the measured TID amplitudes and wave vectors from the TIDDBIT system, together with ion-neutral theory, GW dissipative polarization relations and ray tracing, we determine the GW neutral horizontal wind and density perturbations as a function of altitude from 220 to 380 km. We find that there is a serious discrepancy between the GW dissipative theory and the observations unless the molecular viscosity, μ, decreases with altitude in the middle to upper thermosphere. Assuming that μ∝ρ¯q, where ρ¯ is the density, we find using GW dissipative theory that the GWs could have been observed at zobs and that one or more of the GWs could have caused the uH' wind peak at z≃325 km if q ˜ 0.67 for z≥220 km. This implies that the kinematic viscosity, ν=μ/ρ¯, increases less rapidly with altitude for z≥220 km: ν∝1/ρ¯0.33. This dependence makes sense because as ρ¯→0, the distance between molecules goes to infinity, which implies no molecular collisions and therefore no molecular viscosity μ.

Recent divergences in stratospheric water vapor measurements by frost point hygrometers and the Aura Microwave Limb Sounder.

Science.gov (United States)

Hurst, Dale F; Read, William G; Vömel, Holger; Selkirk, Henry B; Rosenlof, Karen H; Davis, Sean M; Hall, Emrys G; Jordan, Allen F; Oltmans, Samuel J

2016-09-08

Balloon-borne frost point hygrometers (FPs) and the Aura Microwave Limb Sounder (MLS) provide high-quality vertical profile measurements of water vapor in the upper troposphere and lower stratosphere (UTLS). A previous comparison of stratospheric water vapor measurements by FPs and MLS over three sites - Boulder, Colorado (40.0° N); Hilo, Hawaii (19.7° N); and Lauder, New Zealand (45.0° S) - from August 2004 through December 2012 not only demonstrated agreement better than 1% between 68 and 26 hPa but also exposed statistically significant biases of 2 to 10% at 83 and 100 hPa (Hurst et al., 2014). A simple linear regression analysis of the FP-MLS differences revealed no significant long-term drifts between the two instruments. Here we extend the drift comparison to mid-2015 and add two FP sites - Lindenberg, Germany (52.2° N), and San José, Costa Rica (10.0° N) - that employ FPs of different manufacture and calibration for their water vapor soundings. The extended comparison period reveals that stratospheric FP and MLS measurements over four of the five sites have diverged at rates of 0.03 to 0.07 ppmv year -1 (0.6 to 1.5% year -1 ) from ~2010 to mid-2015. These rates are similar in magnitude to the 30-year (1980-2010) average growth rate of stratospheric water vapor (~ 1% year -1 ) measured by FPs over Boulder (Hurst et al., 2011). By mid-2015, the FP-MLS differences at some sites were large enough to exceed the combined accuracy estimates of the FP and MLS measurements.

Radiation budget studies using collocated observations from advanced Very High Resolution Radiometer, High-Resolution Infrared Sounder/2, and Earth Radiation Budget Experiment instruments

Science.gov (United States)

Ackerman, Steven A.; Frey, Richard A.; Smith, William L.

1992-01-01

Collocated observations from the Advanced Very High Resolution Radiometer (AVHRR), High-Resolution Infrared Sounder/2 (HIRS/2), and Earth Radiation Budget Experiment (ERBE) instruments onboard the NOAA 9 satellite are combined to describe the broadband and spectral radiative properties of the earth-atmosphere system. Broadband radiative properties are determined from the ERBE observations, while spectral properties are determined from the HIRS/2 and AVHRR observations. The presence of clouds, their areal coverage, and cloud top pressure are determined from a combination of the HIRS/2 and the AVHRR observations. The CO2 slicing method is applied to the HIRS/2 to determine the presence of upper level clouds and their effective emissivity. The AVHRR data collocated within the HIRS/2 field of view are utilized to determine the uniformity of the scene and retrieve sea surface temperature. Changes in the top of the atmosphere longwave and shortwave radiative energy budgets, and the spectral distribution of longwave radiation are presented as a function of cloud amount and cloud top pressure. The radiative characteristics of clear sky conditions over oceans are presented as a function of sea surface temperature and atmospheric water vapor structure.

Ducted electromagnetic waves in the Martian ionosphere detected by the Mars Advanced Radar for Subsurface and Ionosphere Sounding radar

Science.gov (United States)

Zhang, Zhenfei; Orosei, Roberto; Huang, Qian; Zhang, Jie

2016-07-01

In the data of the Mars Advanced Radar for Subsurface and Ionosphere Sounding on board the European Space Agency (ESA) mission Mars Express (MEX), a distinctive type of signals (called the "epsilon signature"), which is similar to that previously detected during radio sounding of the terrestrial F region ionosphere, is found. The signature is interpreted to originate from multiple reflections of electromagnetic waves propagating along sounder pulse-created, crustal magnetic field-aligned plasma bubbles (waveguides). The signatures have a low (below 0.5%) occurrence rate and apparent cutoff frequencies 3-5 times higher than the theoretical one for an ordinary mode wave. These properties are explained by the influence of the perpendicular ionospheric plasma density gradient and the sounder pulse frequency on the formation of waveguides.

"Aasta betoonehitis 2015" ülejäänud nominendid

Index Scriptorium Estoniae

2016-01-01

Jõhvi riigigümnaasium, Maxima logistikakeskus, Võru riigigümnaasium, Rakvere Tark Maja, Viljandi krematoorium-tavandisaal, Marsi tänava korterelamud Tallinnas Tondil, Kultuurikatel Tallinnas, Kentmanni tn 6 elu- ja ärihoone Tallinnas, Kehärata rongitunneli kaarelemendid Helsingi lennujaama all, Kroodi viaduktid Loo-Maardu teelõigul, ühepereelamu Põldmarja 12 Tartus

Остальные номинанты на премию "Лучшее бетонное строение 2015"

Index Scriptorium Estoniae

2016-01-01

Jõhvi riigigümnaasium, Maxima logistikakeskus, Võru riigigümnaasium, Rakvere Tark Maja, Viljandi krematoorium-tavandisaal, Marsi tänava korterelamud Tallinnas Tondil, Kultuurikatel Tallinnas, Kentmanni tn 6 elu- ja ärihoone Tallinnas, Kehärata rongitunneli kaarelemendid Helsingi lennujaama all, Kroodi viaduktid Loo-Maardu teelõigul, ühepereelamu Põldmarja 12 Tartus

Development Challenges of Utilizing a Corner Cube Mechanism Design with Successful IASI Flight Heritage for the Infrared Sounder (IRS) on MTG: Recurrent Mechanical Design not Correlated to Recurrent Development

Science.gov (United States)

Spanoudakis, Peter; Schwab, Philippe; Kiener, Lionel; Saudan, Herve; Perruchoud, Gerald

2015-09-01

The Corner Cube Mechanism (CCM) design for the Infra-Red Sounder (IRS) on MTG is based on the successful mechanism currently in orbit on the Infrared Atmospheric Sounding Interferometers (IASI) on the Metop satellites. The overall CCM performance is described with attention given to the specific design developments for the MTG project. A description is presented of the modifications introduced and challenges encountered to adapt the IASI space heritage design (which is only 15 years old) to meet the MTG specifications. A detailed account is provided regarding the tests performed on the adapted components for the new programme. The major issues encountered and solutions proposed are illustrated concerning the voice- coil actuator development, optical switch design, fatigue life of the flexure components and the adaptation of the launch locking device. Nevertheless, an Engineering Qualification Model was rapidly manufactured and now undergoing a qualification test campaign.

Four Decades of Space-Borne Radio Sounding

Science.gov (United States)

Benson, Robert F.

2010-01-01

A review is given of the 38 rocket, satellite, and planetary payloads dedicated to ionospheric/magnetospheric radio sounding since 1961. Between 1961 and 1995, eleven sounding-rocket payloads from four countries evolved from proof-of-concept flights to sophisticated instruments. Some involved dual payloads, with the sounder transmitter on one and the sounder receiver on the other. The rocket sounders addressed specific space-plasma-wave questions, and provided improved measurements of ionospheric electron-density (N(sub e)) field-aligned irregularities (FAI). Four countries launched 12 ionospheric topside-sounder satellites between 1962 and 1994, and an ionospheric sounder was placed on the Mir Space Station in 1998. Eleven magnetospheric radio sounders, most of the relaxation type, were launched from 1977 to 2000. The relaxation sounders used low-power transmitters, designed to stimulate plasma resonances for accurate local Ne determinations. The latest magnetospheric sounder designed for remote sensing incorporated long antennas and digital signal processing techniques to overcome the challenges posed by low Ne values and large propagation distances. Three radio sounders from three countries were included on payloads to extraterrestrial destinations from 1990 to 2003. The scientific accomplishments of space-borne radio sounders included (1) a wealth of global N(sub e) information on the topside ionosphere and magnetosphere, based on vertical and magnetic-field-aligned N(sub e) profiles; (2) accurate in-situ N(sub e) values, even under low-density conditions; and (3) fundamental advances in our understanding of the excitation and propagation of plasma waves, which have even led to the prediction of a new plasma-wave mode.

Mars, accessing the third dimension: a software tool to exploit Mars ground penetrating radars data.

Science.gov (United States)

Cantini, Federico; Ivanov, Anton B.

2016-04-01

The Mars Advanced Radar for Subsurface and Ionosphere Sounding (MARSIS), on board the ESA's Mars Express and the SHAllow RADar (SHARAD), on board the NASA's Mars Reconnaissance Orbiter are two ground penetrating radars (GPRs) aimed to probe the crust of Mars to explore the subsurface structure of the planet. By now they are collecting data since about 10 years covering a large fraction of the Mars surface. On the Earth GPRs collect data by sending electromagnetic (EM) pulses toward the surface and listening to the return echoes occurring at the dielectric discontinuities on the planet's surface and subsurface. The wavelengths used allow MARSIS EM pulses to penetrate the crust for several kilometers. The data products (Radargrams) are matrices where the x-axis spans different sampling points on the planet surface and the y-axis is the power of the echoes over time in the listening window. No standard way to manage this kind of data is established in the planetary science community and data analysis and interpretation require very often some knowledge of radar signal processing. Our software tool is aimed to ease the access to this data in particular to scientists without a specific background in signal processing. MARSIS and SHARAD geometrical data such as probing point latitude and longitude and spacecraft altitude, are stored, together with relevant acquisition metadata, in a geo-enabled relational database implemented using PostgreSQL and PostGIS. Data are extracted from official ESA and NASA released data using self-developed python classes and scripts and inserted in the database using OGR utilities. This software is also aimed to be the core of a collection of classes and script to implement more complex GPR data analysis. Geometrical data and metadata are exposed as WFS layers using a QGIS server, which can be further integrated with other data, such as imaging, spectroscopy and topography. Radar geometry data will be available as a part of the iMars Web

Imaging the Antarctic Ice Sheet Subsurface with the HF GPR TAPIR

Science.gov (United States)

Le Gall, A.; Ciarletti, V.; Berthelier, J.; Reineix, A.; Ney, R.; Bonaimé, S.; Corbel, C.

2006-12-01

An HF impulse polarimetric Ground Penetrating Radar (GPR) operating at very low frequencies (ranging from ~2 to 8MHz) has been developed in the frame of the NetLander mission. This instrument, named TAPIR (Terrestrial And Planetary Investigation by Radar), was designed to probe the Martian subsurface down to kilometric depth and search for potential water reservoirs. Although the NetLander mission was cancelled in 2003, the interest on the exploration of Martian subsurface was recently enhanced by the promising observations of the Mars Advanced Radar for Subsurface and Ionospheric Sounding (MARSIS) on board of the ESA Mars Express orbiter. In particular, MARSIS detected the base of the North Polar Layered Deposits, penetrating up to ~1.8km the ice-rich upper layer of the underground. Such results suggest that TAPIR, which operates in the same frequency range as MARSIS and can performed a higher number of coherent integrations, is able to reach deeper structures. Yet, in contrast with classical GPRs, TAPIR can not move onto the surface and thus won't provide 2D or 3D scan of the subsurface. To retrieve, in spite of this NetLander restraint, the 3D distribution of the reflecting facets of the underground, the instrument was equipped with two electrical dipoles and a rotating magnetic sensor. These antennas allow to derive, from the measured values of 5 components of the wave field, the direction of arrival of the reflected waves hence the inclination of the buried reflectors. The first validation of this innovative concept was carried out during the RANETA (RAdar of NEtlander in Terre Adélie) campaign organized by the Institute Paul-Emile Victor in January-February 2004. This campaign took place on the Antarctic ice sheet close to the French-Italian Cap Prudhomme station. 8 soundings of the ice shelf were performed on various sites corresponding to different altitudes above the sea level (ranging from ~285m to ~1100m). We shall provide a detailed description of the

Tropospheric Carbon Monoxide Measurements from the Scanning High-resolution Interferometer Sounder on 7 September 2000 in Southern Africa during SAFARI 2000

Science.gov (United States)

McMillan, W. W.; McCourt, M. L.; Revercomb, H. E.; Knuteson, R. O.; Christian, T. J.; Doddridge, B. G.; Hobbs, P. V.; Lukovich, P. C.; Novelli, P. C.; Piketh, S. J.

2003-01-01

Retrieved tropospheric carbon monoxide (CO) column densities are presented for more than 9000 spectra obtained by the University of Wisconsin-Madison (UWis) Scanning High-Resolution Interferometer Sounder (SHIS) during a flight on the NASA ER-2 on 7 September 2000 as part of the Southern African Regional Science Initiative (SAFARI 2000) dry season field campaign. Enhancements in tropospheric column CO were detected in the vicinity of a controlled biomass burn in the Timbavati Game Reserve in northeastern South Africa and over the edge of the river of smoke in south central Mozambique. Relatively clean air was observed over the far southern coast of Mozambique. Quantitative comparisons are presented with in situ measurements from five different instruments flying on two other aircraft: the University of Washington Convair-580 (CV) and the South African Aerocommander JRB in the vicinity of the Timbavati fire. Measured tropospheric CO columns (extrapolated from 337 to 100 mb) of 2.1 x 10(exp 18) per square centimeter in background air and up to 1.5 x 10(exp 19) per square centimeter in the smoke plume agree well with SHIS retrieved tropospheric CO columns of (2.3 plus or minus 0.25) x 10(exp 18) per square centimeter over background air near the fire and (1.5 plus or minus 0.35) x 10(exp 19) per square centimeter over the smoke plume. Qualitative comparisons are presented with three other in situ CO profiles obtained by the South African JRA aircraft over Mozambique and northern South Africa showing the influence of the river of smoke.

Retrieved Products from Simulated Hyperspectral Observations of a Hurricane

Science.gov (United States)

Susskind, Joel; Kouvaris, Louis; Iredell, Lena; Blaisdell, John

2015-01-01

Demonstrate via Observing System Simulation Experiments (OSSEs) the potential utility of flying high spatial resolution AIRS class IR sounders on future LEO and GEO missions.The study simulates and analyzes radiances for 3 sounders with AIRS spectral and radiometric properties on different orbits with different spatial resolutions: 1) Control run 13 kilometers AIRS spatial resolution at nadir on LEO in Aqua orbit; 2) 2 kilometer spatial resolution LEO sounder at nadir ARIES; 3) 5 kilometers spatial resolution sounder on a GEO orbit, radiances simulated every 72 minutes.

The Application of a Multi-Beam Echo-Sounder in the Analysis of the Sedimentation Situation of a Large Reservoir after an Earthquake

Directory of Open Access Journals (Sweden)

Zhong-Luan Yan

2018-04-01

Full Text Available The Wenchuan Earthquake took place in the upper reach catchment of the Min River. It resulted in large amounts of loose materials gathering in the river channel, leading to changes in the sediment transport system in this area. The Zipingpu Reservoir is the last and the largest reservoir located in the upper reach of the Min River. It is near the epicenter and receives sediment from upstream. This paper puts forward a study on the reservoir sedimentation and storage capacity of the Zipingpu Reservoir, employing a multi-beam echo-sounder system in December 2012. Then, the data were merged with digital line graphics and shuttle radar topography mission data in ArcGIS to build a digital elevation model and triangulate the irregular network of Zipingpu Reservoir. Via the analysis of the bathymetric data, the results show the following: (1 The main channels of the reservoir gradually aggrade to a flat bottom from the deep-cutting valley. Sedimentation forms a reach with a W-shaped longitudinal thalweg profile and an almost zero slope reach in the upstream section of the reservoir due to the natural barrier induced by a landslide; (2 The loss ratios of the wetted cross-section surface are higher than 10% in the upstream section of the reservoir and higher than 40% in the natural barrier area; (3 Comparing the surveyed area storage capacity of December 2012 with March 2008, the Zipingpu Reservoir has lost 15.28% of its capacity at the dead storage water level and 10.49% of its capacity at the flood limit water level.

Recreational Fish-Finders--An Inexpensive Alternative to Scientific Echo-Sounders for Unravelling the Links between Marine Top Predators and Their Prey.

Directory of Open Access Journals (Sweden)

Alistair M McInnes

Full Text Available Studies investigating how mobile marine predators respond to their prey are limited due to the challenging nature of the environment. While marine top predators are increasingly easy to study thanks to developments in bio-logging technology, typically there is scant information on the distribution and abundance of their prey, largely due to the specialised nature of acquiring this information. We explore the potential of using single-beam recreational fish-finders (RFF to quantify relative forage fish abundance and draw inferences of the prey distribution at a fine spatial scale. We compared fish school characteristics as inferred from the RFF with that of a calibrated scientific split-beam echo-sounder (SES by simultaneously operating both systems from the same vessel in Algoa Bay, South Africa. Customized open-source software was developed to extract fish school information from the echo returns of the RFF. For schools insonified by both systems, there was close correspondence between estimates of mean school depth (R2 = 0.98 and school area (R2 = 0.70. Estimates of relative school density (mean volume backscattering strength; Sv measured by the RFF were negatively biased through saturation of this system given its smaller dynamic range. A correction factor applied to the RFF-derived density estimates improved the comparability between the two systems. Relative abundance estimates using all schools from both systems were congruent at scales from 0.5 km to 18 km with a strong positive linear trend in model fit estimates with increasing scale. Although absolute estimates of fish abundance cannot be derived from these systems, they are effective at describing prey school characteristics and have good potential for mapping forage fish distribution and relative abundance. Using such relatively inexpensive systems could greatly enhance our understanding of predator-prey interactions.

Recreational Fish-Finders—An Inexpensive Alternative to Scientific Echo-Sounders for Unravelling the Links between Marine Top Predators and Their Prey

Science.gov (United States)

McInnes, Alistair M.; Khoosal, Arjun; Murrell, Ben; Merkle, Dagmar; Lacerda, Miguel; Nyengera, Reason; Coetzee, Janet C.; Edwards, Loyd C.; Ryan, Peter G.; Rademan, Johan; van der Westhuizen, Jan J; Pichegru, Lorien

2015-01-01

Studies investigating how mobile marine predators respond to their prey are limited due to the challenging nature of the environment. While marine top predators are increasingly easy to study thanks to developments in bio-logging technology, typically there is scant information on the distribution and abundance of their prey, largely due to the specialised nature of acquiring this information. We explore the potential of using single-beam recreational fish-finders (RFF) to quantify relative forage fish abundance and draw inferences of the prey distribution at a fine spatial scale. We compared fish school characteristics as inferred from the RFF with that of a calibrated scientific split-beam echo-sounder (SES) by simultaneously operating both systems from the same vessel in Algoa Bay, South Africa. Customized open-source software was developed to extract fish school information from the echo returns of the RFF. For schools insonified by both systems, there was close correspondence between estimates of mean school depth (R2 = 0.98) and school area (R2 = 0.70). Estimates of relative school density (mean volume backscattering strength; Sv) measured by the RFF were negatively biased through saturation of this system given its smaller dynamic range. A correction factor applied to the RFF-derived density estimates improved the comparability between the two systems. Relative abundance estimates using all schools from both systems were congruent at scales from 0.5 km to 18 km with a strong positive linear trend in model fit estimates with increasing scale. Although absolute estimates of fish abundance cannot be derived from these systems, they are effective at describing prey school characteristics and have good potential for mapping forage fish distribution and relative abundance. Using such relatively inexpensive systems could greatly enhance our understanding of predator-prey interactions. PMID:26600300

A comprehensive overview of the climatological composition of the Asian summer monsoon anticyclone based on 10 years of Aura Microwave Limb Sounder measurements

Science.gov (United States)

Santee, M. L.; Manney, G. L.; Livesey, N. J.; Schwartz, M. J.; Neu, J. L.; Read, W. G.

2017-05-01

Intense deep convection associated with the Asian summer monsoon (ASM) lofts surface pollutants to the upper troposphere/lower stratosphere (UTLS), where strong winds and long chemical lifetimes allow intercontinental transport, affecting atmospheric composition around the globe. The Aura Microwave Limb Sounder (MLS), launched in 2004, makes simultaneous colocated measurements of trace gases and cloud ice water content (a proxy for deep convection) in the UTLS on a daily basis. Here we exploit the dense spatial and temporal coverage, long-term data record, extensive measurement suite, and insensitivity to aerosol and most clouds of Aura MLS to characterize the climatological (2005-2014) composition of the ASM anticyclone throughout its annual life cycle. We use version 4 MLS data to quantify spatial and temporal variations in both tropospheric (H2O, CO, CH3Cl, CH3CN, CH3OH) and stratospheric (O3, HNO3, HCl) tracers on four potential temperature surfaces (350-410 K). Inside the mature anticyclone, all species exhibit substantial changes, not only from their premonsoon distributions in the ASM region but also from their summertime distributions in the rest of the hemisphere. Different tracers exhibit dissimilar seasonal evolution, and the exact location and timing of their extreme values vary. Although individual aspects of the anticyclone have been described previously, we present a uniquely comprehensive overview of the climatological seasonal evolution of the ASM and its impact on UTLS composition. This work provides valuable context for planned in situ measurements as well as a benchmark for model evaluation and future investigations of interannual variability and long-term changes in monsoon processes.

Analytically derived conversion of spectral band radiance to brightness temperature

Energy Technology Data Exchange (ETDEWEB)

Berk, Alexander [Spectral Sciences, Inc., 44th Avenue, Burlington, MA 01803 (United States)], E-mail: lex@spectral.com

2008-05-15

Simple analytic expressions for brightness temperature have been derived in terms of band response function spectral moments. Accuracy measures are also derived. Application of these formulas to GOES-12 Sounder thermal infrared bands produces brightness temperature residuals between -5.0 and 2.5 mK for a 150-400 K temperature range. The magnitude of residuals for the five ASTER Radiometer thermal infrared bands over the same temperature range is less than 0.22 mK.

Atmospheric Infrared Sounder on NASA's Aqua Satellite: Applications for Volcano Rapid Response, Influenza Outbreak Prediction, and Drought Onset Prediction

Science.gov (United States)

Ray, S. E.; Fetzer, E. J.; Lambrigtsen, B.; Olsen, E. T.; Licata, S. J.; Hall, J. R.; Penteado, P. F.; Realmuto, V. J.; Thrastarson, H. T.; Teixeira, J.; Granger, S. L.; Behrangi, A.; Farahmand, A.

2017-12-01

The Atmospheric Infrared Sounder (AIRS) has been returning daily global observations of Earth's atmospheric constituents and properties since 2002. With its 15-year data record and near real-time capability, AIRS data are being used in the development of applications that fall within many of the NASA Applied Science focus areas. An automated alert system for volcanic plumes has been developed that triggers on threshold breaches of SO2, ash and dust in granules of AIRS data. The system generates a suite of granule-scale maps that depict both plume and clouds, all accessible from the AIRS web site. Alerts are sent to a curated list of volcano community members, and links to views in NASA Worldview and Google Earth are also available. Seasonal influenza epidemics are major public health concern with millions of cases of severe illness and large economic impact. Recent studies have highlighted the role of absolute or specific humidity as a likely player in the seasonal nature of these outbreaks. A quasi-operational influenza outbreak prediction system has been developed based on the SIRS model which uses AIRS and NCEP humidity data, Center for Disease Control reports on flu and flu-like illnesses, and results from Google Flu Trends. Work is underway to account for diffusion (spatial) in addition to the temporal spreading of influenza. The US Drought Monitor (USDM) is generated weekly by the National Drought Mitigation Center (NDMC) and is used by policymakers for drought decision-making. AIRS data have demonstrated utility in monitoring the development and detection of meteorological drought with both AIRS-derived standardized vapor pressure deficit and standardized relative humidity, showing early detection lead times of up to two months. An agreement was secured with the NDMC to begin a trial period using AIRS products in the production of the USDM, and in July of 2017 the operational delivery of weekly CONUS AIRS images of Relative Humidity, Surface Air Temperature

Territoriality of feral pigs in a highly persecuted population on Fort Benning, Georgia

Science.gov (United States)

Sparklin, B.D.; Mitchell, M.S.; Hanson, L.B.; Jolley, D.B.; Ditchkoff, S.S.

2009-01-01

We examined home range behavior of female feral pigs (Sus scrofa) in a heavily hunted population on Fort Benning Military Reservation in west-central Georgia, USA. We used Global Positioning System location data from 24 individuals representing 18 sounders (i.e., F social groups) combined with markrecapture and camera-trap data to evaluate evidence of territorial behavior at the individual and sounder levels. Through a manipulative experiment, we examined evidence for an inverse relationship between population density and home range size that would be expected for territorial animals. Pigs from the same sounder had extensive home range overlap and did not have exclusive core areas. Sounders had nearly exclusive home ranges and had completely exclusive core areas, suggesting that female feral pigs on Fort Benning were territorial at the sounder level but not at the individual level. Lethal removal maintained stable densities of pigs in our treatment area, whereas density increased in our control area; territory size in the 2 areas was weakly and inversely related to density of pigs. Territorial behavior in feral pigs could influence population density by limiting access to reproductive space. Removal strategies that 1) match distribution of removal efforts to distribution of territories, 2) remove entire sounders instead of individuals, and 3) focus efforts where high-quality food resources strongly influence territorial behaviors may be best for long-term control of feral pigs.

Comparison of OLR Data Sets from AIRS, CERES and MERRA 2

Science.gov (United States)

Lee, Jae N.; Susskind, Joel; Iredell, Lena; Loeb, Norman; Lim, Young-Kwon

2015-01-01

Organizers of the NASA Sounder Science Team Meeting would like to post the presentations to a the JPL Atmospheric Infrared Sounder (AIRS) publicly-available website. The meeting was held in Greenbelt, Maryland, October 13-16, 2015.

Remote acoustic seafloor characterization using numerical model and statistical based stochastic multifractals

Digital Repository Service at National Institute of Oceanography (India)

Chakraborty, B.; Haris, K.

The spatial variability of sediment geoacoustic inversion parameters are estimated employing bathymetric systems such as multi-beam echo-sounder (MBES) and dualfrequency single-beam echo-sounder (SBES) operable at 95 kHz and 33/210 kHz, respectively...

Plasma instabilities stimulated by HF transmitters in space

International Nuclear Information System (INIS)

Benson, R.F.; Vinas, A.F.

1988-01-01

Diffuse incoherent signal returns are often observed on Alouette and ISIS topside ionograms in addition to coherent echoes of electromagnetic and electrostatic waves. These diffuse signals, which at times can be the dominant features on topside ionograms, have been attributed to sounder-induced temperature anisotropies which drive the Harris instability. Previous theoretical investigations were based on the electrostatic approximation to the dispersion equation. The present paper will present calculations indicating that when the electromagnetic terms are retained in the dispersion equation and when the sounder-stimulated perpendicular electron temperature approaches 1 keV, then the whistler mode can have a temporal growth rate larger than the electrostatic electron cyclotron harmonic wave mode central to the diffuse resonance problem. Present sounders lack the power and antenna lengths to generate whistler mode waves in this manner. In addition, such waves would have large group velocities and would quickly leave the vicinity of the sounder. Experiments to investigate the wave growth, propagation, and damping of such stimulated waves are planned for the 1990s using a highly flexible sounder on the Space Shuttle and a receiver on a subsatellite. 30 references

Journal of Earth System Science | Indian Academy of Sciences

Indian Academy of Sciences (India)

It is examined whether high resolution radiosonde measurements represent well the UTWV by comparing with different satellite based (Atmospheric Infrared Sounder (AIRS), Advanced Microwave Sounding Unit-B (AMSUB) and Microwave Limb Sounder (MLS)) water vapour measurements. Very good comparison in the ...

Interrelated variations of O3, CO and deep convection in the tropical/subtropical upper troposphere observed by the Aura Microwave Limb Sounder (MLS during 2004–2011

Directory of Open Access Journals (Sweden)

L. Froidevaux

2013-01-01

Full Text Available The interrelated geographic and temporal variability seen in more than seven years of tropical and subtropical upper tropospheric (215 hPa ozone, carbon monoxide and cloud ice water content (IWC observations by the Aura Microwave Limb Sounder (MLS are presented. Observed ozone abundances and their variability (geographic and temporal agree to within 10–15 ppbv with records from sonde observations. MLS complements these (and other observations with global coverage and simultaneous measurements of related parameters. Previously-reported phenomena such as the ozone "wave one" feature are clearly seen in the MLS observations, as is a double peak in ozone abundance over tropical East Africa, with enhanced abundances in both May to June and September to November. While repeatable seasonal cycles are seen in many regions, they are often accompanied by significant interannual variability. Ozone seasonal cycles in the southern tropics and subtropics tend to be more distinct (i.e., annually repeatable than in the northern. By contrast, carbon monoxide shows distinct seasonal cycles in many northern subtropical regions, notably from India to the Eastern Pacific. Deep convection (as indicated by large values of IWC is typically associated with reductions in upper tropospheric ozone. Convection over polluted regions is seen to significantly enhance upper tropospheric carbon monoxide. While some regions show statistically significant correlations among ozone, carbon monoxide and IWC, simple correlations fall well short of accounting for the observed variability. The observed interrelated variations and metrics of annual and interannual variability described here represent a new resource for validation of atmospheric chemistry models.

Contact heterogeneities in feral swine: implications for disease management and future research

Science.gov (United States)

Pepin, Kim; Davis, Amy J.; Beasley, James; Boughton, Raoul; Campbell, Tyler; Cooper, Susan; Gaston, Wes; Hartley, Stephen B.; Kilgo, John C.; Wisely, Samantha; Wyckoff, Christy; VerCauteren, Kurt

2016-01-01

Contact rates vary widely among individuals in socially structured wildlife populations. Understanding the interplay of factors responsible for this variation is essential for planning effective disease management. Feral swine (Sus scrofa) are a socially structured species which pose an increasing threat to livestock and human health, and little is known about contact structure. We analyzed 11 GPS data sets from across the United States to understand the interplay of ecological and demographic factors on variation in co-location rates, a proxy for contact rates. Between-sounder contact rates strongly depended on the distance among home ranges (less contact among sounders separated by >2 km; negligible between sounders separated by >6 km), but other factors causing high clustering between groups of sounders also seemed apparent. Our results provide spatial parameters for targeted management actions, identify data gaps that could lead to improved management and provide insight on experimental design for quantitating contact rates and structure.

Heard Island and McDonald Islands Acoustic Plumes: Split-beam Echo sounder and Deep Tow Camera Observations of Gas Seeps on the Central Kerguelen Plateau

Science.gov (United States)

Watson, S. J.; Spain, E. A.; Coffin, M. F.; Whittaker, J. M.; Fox, J. M.; Bowie, A. R.

2016-12-01

Heard and McDonald islands (HIMI) are two active volcanic edifices on the Central Kerguelen Plateau. Scientists aboard the Heard Earth-Ocean-Biosphere Interactions voyage in early 2016 explored how this volcanic activity manifests itself near HIMI. Using Simrad EK60 split-beam echo sounder and deep tow camera data from RV Investigator, we recorded the distribution of seafloor emissions, providing the first direct evidence of seabed discharge around HIMI, mapping >244 acoustic plume signals. Northeast of Heard, three distinct plume clusters are associated with bubbles (towed camera) and the largest directly overlies a sub-seafloor opaque zone (sub-bottom profiler) with >140 zones observed within 6.5 km. Large temperature anomalies did not characterize any of the acoustic plumes where temperature data were recorded. We therefore suggest that these plumes are cold methane seeps. Acoustic properties - mean volume backscattering and target strength - and morphology - height, width, depth to surface - of plumes around McDonald resembled those northeast of Heard, also suggesting gas bubbles. We observed no bubbles on extremely limited towed camera data around McDonald; however, visibility was poor. The acoustic response of the plumes at different frequencies (120 kHz vs. 18 kHz), a technique used to classify water column scatterers, differed between HIMI, suggestiing dissimilar target size (bubble radii) distributions. Environmental context and temporal characteristics of the plumes differed between HIMI. Heard plumes were concentrated on flat, sediment rich plains, whereas around McDonald plumes emanated from sea knolls and mounds with hard volcanic seafloor. The Heard plumes were consistent temporally, while the McDonald plumes varied temporally possibly related to tides or subsurface processes. Our data and analyses suggest that HIMI acoustic plumes were likely caused by gas bubbles; however, the bubbles may originate from two or more distinct processes.

determination of volume and direction of flow of kainji reservoir ...

African Journals Online (AJOL)

HOD

enough to fix position of points aboard a boat traversing a river during field work. In this paper, MIDAS Echo Sounder and Trimble Dual Frequency GPS was used for data acquisition. Data analysis was done with the aid of Surfer golden .... hydrographic system included a GPS receiver with a built-in radio, a depth sounder, ...

AMOS Seeing Quality Measurements.

Science.gov (United States)

1976-01-01

Measurement Atmosphere Turbulence Propagation Observatory Acoustic Sounder Maui Optical Station Astronomy Microthermal Probe TEAL BLUE Degradation...presence of the site structures. Instruments used were an acoustic sounder (to probe the altitudes of 100 to 1000 ft), fine- wire microthermal probes...responsibility during the experiment for the microthermal probes. The report itself, while compiled and edited at RADC, may be considered as coming from

NOAA Interest in Small Satellite Solutions for Mitigation of Data Gaps

Science.gov (United States)

Caulfield, M.; Tewey, K.; John, P.

2016-12-01

The National Oceanic and Atmospheric Administration (NOAA) is undertaking a strategy to achieve satellite constellation robustness by 2023 to maintain continuity of polar satellite observations, which are central to NOAA's weather forecast capability. NOAA's plans include mitigation activities in the event of a loss of polar observations. In 2017, NOAA will begin development of the Earth Observing Nanosatellite - Microwave (EON-MW). EON-MW is a miniature microwave sounder that approximates the atmospheric profiling capabilities of the Advanced Technology Microwave Sounder (ATMS) instrument on the NOAA Joint Polar Satellite System (JPSS). NOAA is collaborating with the Massachusetts Institute of Technology's Lincoln Laboratory (MIT / LL) on EON-MW, which includes 2 years of risk reduction efforts to further define the EON-MW mission and identify and manage key technical risks. These studies will refine designs and evaluate system trades for operational earth observations from a U-class satellite platform, as well as examine microwave sensor concepts and investigated payload architecture to support microwave frequencies for atmospheric remote sensing. Similar to EON-MW, NOAA is also investigating the potential to mitigate against the loss of the JPSS Cross Track Infrared Sounder (CrIS) data with a CubeSat based mid-wave Infrared sounder. NOAA is collaborating with the Jet Propulsion Laboratory (JPL) to design the Earth Observation Nanosatellite-Infrared (EON-IR). EON-IR will leverage the NASA-JPL CubSat based infrared sounder CubSat Infrared Atmospheric Sounder (CIRAS) mission. In FY 2015 NOAA funded a study to analyze the feasibility of meeting the essential requirements of the CrIS from a CubeSat platform and began exploring the basic design of the EON-IR payload and bus. NOAA will continue to study EON-IR in 2016 by examining ways to modify the CIRAS design to better meet NOAA's observational and operational needs. These modifications will aim to increase mission

The change of expression configuration affects identity-dependent expression aftereffect but not identity-independent expression aftereffect

Directory of Open Access Journals (Sweden)

Miao eSong

2015-12-01

Full Text Available The present study examined the influence of expression configuration on cross-identity expression aftereffect. The expression configuration refers to the spatial arrangement of facial features in a face for conveying an emotion, e.g., an open-mouth smile versus a closed-mouth smile. In the first of two experiments, the expression aftereffect is measured using across-identity/cross-expression configuration factorial design. The facial identities of test faces were the same or different from the adaptor, while orthogonally, the expression configurations of those facial identities were also the same or different. The result shows that the change of expression configuration impaired the expression aftereffect when the facial identities of adaptor and tests were the same; however, the impairment effect disappears when facial identities were different, indicating the identity-independent expression representation is more robust to the change of the expression configuration in comparison with the identity-dependent expression representation. In the second experiment, we used schematic line faces as adaptors and real faces as tests to minimize the similarity between the adaptor and tests, which is expected to exclude the contribution from the identity-dependent expression representation to expression aftereffect. The second experiment yields a similar result as the identity-independent expression aftereffect observed in Experiment 1. The findings indicate the different neural sensitivities to expression configuration for identity-dependent and identity-independent expression systems.

Anksioznost učencev 6. razreda osnovne šole pri pouku angleščine kot tujega jezika v povezavi z njihovo učno uspešnostjo

OpenAIRE

Praček, Anja

2017-01-01

Magistrsko delo obravnava anksioznost šestošolcev pri pouku angleščine kot tujega jezika v povezavi z njihovo učno uspešnostjo pri pouku angleščine. Pouk angleščine se v marsičem razlikuje od drugih šolskih predmetov in prav ta posebna učna situacija lahko pri učencih izzove anksioznost. Magistrsko delo je sestavljeno iz teoretičnega in empiričnega dela. V teoretičnem delu je s pomočjo literature na kratko predstavljena trenutna situacija poučevanja in učenja angleščine v slovenskih javnih...

Temperature Anomalies from the AIRS Product in Giovanni for the Climate Community

Science.gov (United States)

Ding, Feng; Hearty, Thomas J.; Wei, Jennifer; Theobald, Michael; Vollmer, Bruce; Seiler, Edward; Meyer, David

2018-01-01

The Atmospheric Infrared Sounder (AIRS) mission began with the launch of Aqua in 2002. Over 15 years of AIRS products have been used by the climate research and application communities. The NASA Goddard Earth Sciences Data and Information Services Center (GES DISC), in collaboration with NASA Sounder Team at JPL, provides processing, archiving, and distribution services for NASA sounders: the present Aqua AIRS mission and the succeeding Suomi National Polar-Orbiting Partnership (SNPP) Cross-track Infrared Sounder (CrIS) mission. We generated a Multi-year Monthly Mean and Anomaly product using 14 years of AIRS standard monthly product. The product includes Air Temperature at the Surface and Surface Skin Temperature, both in Ascending/Daytime and Descending/Nighttime mode. The temperature variables and their anomalies are deployed to Giovanni, a Web-based application developed by the GES DISC. Giovanni provides a simple and intuitive way to visualize, analyze, and access vast amounts of Earth science remote sensing data without having to download the data. It is also a powerful tool that stakeholders can use for decision support in planning and preparing for increased climate variability. In this presentation, we demonstrate the functions in Giovanni with use cases employing AIRS Multi-year Monthly Mean and Anomaly variables.

Cloud and Thermodynamic Parameters Retrieved from Satellite Ultraspectral Infrared Measurements

Science.gov (United States)

Zhou, Daniel K.; Smith, William L.; Larar, Allen M.; Liu, Xu; Taylor, Jonathan P.; Schluessel, Peter; Strow, L. Larrabee; Mango, Stephen A.

2008-01-01

Atmospheric-thermodynamic parameters and surface properties are basic meteorological parameters for weather forecasting. A physical geophysical parameter retrieval scheme dealing with cloudy and cloud-free radiance observed with satellite ultraspectral infrared sounders has been developed and applied to the Infrared Atmospheric Sounding Interferometer (IASI) and the Atmospheric InfraRed Sounder (AIRS). The retrieved parameters presented herein are from radiance data gathered during the Joint Airborne IASI Validation Experiment (JAIVEx). JAIVEx provided intensive aircraft observations obtained from airborne Fourier Transform Spectrometer (FTS) systems, in-situ measurements, and dedicated dropsonde and radiosonde measurements for the validation of the IASI products. Here, IASI atmospheric profile retrievals are compared with those obtained from dedicated dropsondes, radiosondes, and the airborne FTS system. The IASI examples presented here demonstrate the ability to retrieve fine-scale horizontal features with high vertical resolution from satellite ultraspectral sounder radiance spectra.

Mars Express en route for the Red Planet

Science.gov (United States)

2003-06-01

remainder of the time, at Earth to relay the information collected in this way and the data transmitted by Beagle 2. The orbiter’s seven on-board instruments are expected to provide considerable information about the structure and evolution of Mars. A very high resolution stereo camera, the HRSC, will perform comprehensive mapping of the planet at 10 m resolution and will even be capable of photographing some areas to a precision of barely 2 m. The OMEGA spectrometer will draw up the first mineralogical map of the planet to 100 m precision. This mineralogical study will be taken further by the PFS spectrometer - which will also chart the composition of the Martian atmosphere, a prerequisite for investigation of atmospheric dynamics. The MARSIS radar instrument, with its 40 m antenna, will sound the surface to a depth of 2 km, exploring its structure and above all searching for pockets of water. Another instrument, ASPERA, will be tasked with investigating interaction between the upper atmosphere and the interplanetary medium. The focus here will be on determining how and at what rate the solar wind, in the absence of a magnetic field capable of deflecting it, scattered the bulk of the Martian atmosphere into space. Atmospheric investigation will also be performed by the SPICAM spectrometer and the MaRS experiment, with special emphasis on stellar occultation and radio signal propagation phenomena. The orbiter mission should last at least one Martian year (687 days), while Beagle 2 is expected to operate on the planet’s surface for 180 days. Only a start to exploration This first European mission to Mars incorporates some of the objectives of the Euro-Russian Mars 96 mission, which came to grief when the Proton launcher failed. And indeed a Russian partner is cooperating on each of the orbiter’s instruments. Mars Express forms part of an international Mars exploration programme, featuring also the US probes Mars Surveyor and Mars Odyssey, the two Mars Exploration

Expression

Directory of Open Access Journals (Sweden)

Wang-Xia Wang

2014-02-01

Full Text Available The miR-15/107 family comprises a group of 10 paralogous microRNAs (miRNAs, sharing a 5′ AGCAGC sequence. These miRNAs have overlapping targets. In order to characterize the expression of miR-15/107 family miRNAs, we employed customized TaqMan Low-Density micro-fluid PCR-array to investigate the expression of miR-15/107 family members, and other selected miRNAs, in 11 human tissues obtained at autopsy including the cerebral cortex, frontal cortex, primary visual cortex, thalamus, heart, lung, liver, kidney, spleen, stomach and skeletal muscle. miR-103, miR-195 and miR-497 were expressed at similar levels across various tissues, whereas miR-107 is enriched in brain samples. We also examined the expression patterns of evolutionarily conserved miR-15/107 miRNAs in three distinct primary rat brain cell preparations (enriched for cortical neurons, astrocytes and microglia, respectively. In primary cultures of rat brain cells, several members of the miR-15/107 family are enriched in neurons compared to other cell types in the central nervous system (CNS. In addition to mature miRNAs, we also examined the expression of precursors (pri-miRNAs. Our data suggested a generally poor correlation between the expression of mature miRNAs and their precursors. In summary, we provide a detailed study of the tissue and cell type-specific expression profile of this highly expressed and phylogenetically conserved family of miRNA genes.

AMOS Phase IV Annual Report

Science.gov (United States)

1980-06-01

Microthermal Probe System; 2) Acoustic Sounder; 3) Star Sensor; and i 4) Seeing Monitor. Thý in ..ru. cn.tat -ion, much of it one-of-a-kind prototype...profiles of C 2 N2 3) Acoustic Sounder: an instrument that measures C to 300 m altitude; 4) Microthermal Probes: two systems, consisting of three 2...atmospheric program produced - 146 - Y- MICROTHERMAL NICRPHMERI-AL PROBES (3) _j PPRBES (3) WIND SPEED & WIND SPEED & DIRECTION ---- I- DIRECTION

Deep Interior Mission: Imaging the Interior of Near-Earth Asteroids Using Radio Reflection Tomography

Science.gov (United States)

Safaeinili, A.; Asphaug, E.; Belton, M.; Klaasen, K.; Ostro, S.; Plaut, J.; Yeomans, D.

2004-12-01

Near-Earth asteroids are important exploration targets since they provide clues to the evolution of the solar system. They are also of interest since they present a clear danger to Earth in the future. Our mission objective is to image the internal structure of two NEOs using radio reflection tomography (RRT), in order to explore the record of asteroid origin and impact evolution, and to test the fundamental hypothesis that these important members of the solar system are rubble piles rather than consolidated bodies. Our mission's RRT technique is analogous to doing a ``CAT scan" of the asteroid from orbit. Closely sampled radar echoes are processed to yield volumetric maps of mechanical and compositional boundaries, and measure interior material dielectric properties. The RRT instrument is a radar that operates at 5 and 15 MHz with two 30-m (tip-to-tip) dipole antennas that are used in a cross-dipole configuration. The radar transmitter and receiver electronics have heritage from JPL's MARSIS contribution to Mars Express, and the antenna is similar to systems used in IMAGE and LACE missions. The 5-MHz channel is designed to penetrate >1 km of basaltic rock, and 15-MHz penetrates a few hundred meters or more. In addition to RRT volumetric imaging, we use a redundant color cameras to explore the surface expressions of unit boundaries, in order to relate interior radar imaging to what is observable from spacecraft imaging and from Earth. The camera also yields stereo color imaging for geology and RRT-related compositional analysis. Gravity and high fidelity geodesy are used to explore how interior structure is expressed in shape, density, mass distribution and spin. Deep interior has two targets (S-type 1999 ND43 and V-type Nyx ) whose composition bracket the diversity of solar system materials that we are likely to encounter, and are richly complementary.

Facial expressions recognition with an emotion expressive robotic head

Science.gov (United States)

Doroftei, I.; Adascalitei, F.; Lefeber, D.; Vanderborght, B.; Doroftei, I. A.

2016-08-01

The purpose of this study is to present the preliminary steps in facial expressions recognition with a new version of an expressive social robotic head. So, in a first phase, our main goal was to reach a minimum level of emotional expressiveness in order to obtain nonverbal communication between the robot and human by building six basic facial expressions. To evaluate the facial expressions, the robot was used in some preliminary user studies, among children and adults.

Indagini Stratigrafiche e Sistemi Informativi Architettonici: il GIS della chiesa di S. Maria in Valle Porclaneta

Directory of Open Access Journals (Sweden)

Trizio, Ilaria

2009-12-01

Full Text Available The church of Santa Maria in Valle Porclaneta and its accessories are what remains of an ancient Benedictine monastery, built in the heart of Abruzzo region (Magliano dei Marsi, L’Aquila at the end of the XII century. Even if literature, starting from the end of XIX century, has been often interested in this architectural manufactured, the process of historical-critical knowledge of the complex revealed itself difficult due to the absence of archaeological data, the shortage of the documents and, above all, the numerous interventions of restoration that, followed during all last century, have deeply modified the original compages of it. Due to the difficulties met, the overlap between the few documental data and the data derived from the direct investigation of the structures, performed with the aid of an Architectural Informative System, made possible to hypothesize a chronological reconstruction of the principal phases of life of the monastic complex.[it] La chiesa di Santa Maria in Valle Porclaneta e i suoi ambienti accessori sono quanto resta di un antico monastero benedettino, edificato nel cuore del territorio abruzzese (Magliano dei Marsi, L’Aquila sul finire del XII secolo. Sebbene la letteratura, a partire dalla fine dell’Ottocento, si sia spesso occupata di questo manufatto, il processo di conoscenza storico-critica del complesso si è rivelato difficoltoso a causa dell’assenza di dati archeologici, della scarsità di fonti documentarie pervenute, e, soprattutto a causa del susseguirsi di numerosi interventi di restauro che, succedutisi senza soluzione di continuità per tutto il secolo scorso, ne hanno letteralmente stravolto la compagine originale. A fronte delle difficoltà incontrate, la sovrapposizione tra i pochi dati documentali e i dati derivati dall’indagine diretta delle strutture, eseguita con l’ausilio di un Sistema Informativo Architettonico ha consentito di ipotizzare una ricostruzione cronologica delle

Millimeter radiometer system technology

Science.gov (United States)

Wilson, W. J.; Swanson, P. N.

1989-07-01

JPL has had a large amount of experience with spaceborne microwave/millimeter wave radiometers for remote sensing. All of the instruments use filled aperture antenna systems from 5 cm diameter for the microwave Sounder Units (MSU), 16 m for the microwave limb sounder (MLS) to 20 m for the large deployable reflector (LDR). The advantages of filled aperture antenna systems are presented. The requirements of the 10 m Geoplat antenna system, 10 m multified antenna, and the MLS are briefly discussed.

Expressiveness modulo Bisimilarity of Regular Expressions with Parallel Composition (Extended Abstract

Directory of Open Access Journals (Sweden)

Jos C. M. Baeten

2010-11-01

Full Text Available The languages accepted by finite automata are precisely the languages denoted by regular expressions. In contrast, finite automata may exhibit behaviours that cannot be described by regular expressions up to bisimilarity. In this paper, we consider extensions of the theory of regular expressions with various forms of parallel composition and study the effect on expressiveness. First we prove that adding pure interleaving to the theory of regular expressions strictly increases its expressiveness up to bisimilarity. Then, we prove that replacing the operation for pure interleaving by ACP-style parallel composition gives a further increase in expressiveness. Finally, we prove that the theory of regular expressions with ACP-style parallel composition and encapsulation is expressive enough to express all finite automata up to bisimilarity. Our results extend the expressiveness results obtained by Bergstra, Bethke and Ponse for process algebras with (the binary variant of Kleene's star operation.

Short climatology of the atmospheric boundary layer using acoustic methods

International Nuclear Information System (INIS)

Schubert, J.F.

1975-06-01

A climatology of the boundary layer of the atmosphere at the Savannah River Laboratory is being compiled using acoustic methods. The atmospheric phenomenon as depicted on the facsimile recorder is classified and then placed into one of sixteen categories. After classification, the height of the boundary layer is measured. From this information, frequency tables of boundary layer height and category are created and then analyzed for the percentage of time that each category was detected by the acoustic sounder. The sounder also accurately depicts the diurnal cycle of the boundary layer and, depending on the sensitivity of the system, shows microstructure that is normally unavailable using other methods of profiling. The acoustic sounder provides a means for continuous, real time measurements of the time rate of change of the depth of the boundary layer. This continuous record of the boundary layer with its convective cells, gravity waves, inversions, and frontal system passages permits the synoptic and complex climatology of the local area to be compiled. (U.S.)

Validation of vertical profile from atmosphere using ATOVS products and its impact over Indian region.

Science.gov (United States)

Mahandru, Riddhi; Kumar, Adarsh; Mitra, Ashim kumar

This research paper summarizes the validation of atmospheric vertical profile using NOAA(National Oceanic and Atmospheric Administration)/ MetOp satellite derived data over India with radiosonde observations over a span of 8 months. NOAA's International Advanced Television and Infrared Observations satellite Vertical Sounder (ATOVS) processing package (IAPP) obtains temperature and moisture profiles in different pressure levels ranging from 1000hpa to 10hpa from real time direct broadcast (DB) receiving system installed at India Meteorological department. Different pressure levels were substituted to the same pressure levels for calculations of standard deviation, bias and RMSE (root mean square error) The sounder derived products like Total precipitable water vapor (TPW) and Lifting index(LI) from NOAA Satellite was also validated with radiosonde data which provided significant results for weather forecasting. The validation shows that the sounder provides unique information about the state of atmosphere and monitoring the convective environment for severe weather forecasting In addition to this, case study on severe weather events was analyzed using ATOVS products.

Dayside ionosphere of Mars: Empirical model based on data from the MARSIS instrument

Czech Academy of Sciences Publication Activity Database

Němec, F.; Morgan, D. D.; Gurnett, D. A.; Duru, F.; Truhlík, Vladimír

2011-01-01

Roč. 116, - (2011), E07003/1-E07003/14 ISSN 0148-0227 Institutional research plan: CEZ:AV0Z30420517 Keywords : RADIO OCCULTATION MEASUREMENTS * GLOBAL SURVEYOR OBSERVATIONS * MARTIAN IONOSPHERE Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 3.021, year: 2011 http://www.agu.org/pubs/crossref/2011/2010JE003789.shtml

Standard-target calibration of an acoustic backscatter system

Science.gov (United States)

Foote, Kenneth G.; Martini, Marinna A.

2010-01-01

The standard-target method used to calibrate scientific echo sounders and other scientific sonars by a single, solid elastic sphere is being adapted to acoustic backscatter (ABS) systems. Its first application, to the AQUAscat 1000, is described. The on-axis sensitivity and directional properties of transducer beams at three operating frequencies, nominally 1, 2.5, and 4 MHz, have been determined using a 10-mm-diameter sphere of tungsten carbide with 6% cobalt binder. Preliminary results are reported for the 1-MHz transducer. Their application to measurements of suspended sediment made in situ with the same device is described. This will enable the data to be expressed directly in physical units of volume backscattering.

Children's Representations of Facial Expression and Identity: Identity-Contingent Expression Aftereffects

Science.gov (United States)

Vida, Mark D.; Mondloch, Catherine J.

2009-01-01

This investigation used adaptation aftereffects to examine developmental changes in the perception of facial expressions. Previous studies have shown that adults' perceptions of ambiguous facial expressions are biased following adaptation to intense expressions. These expression aftereffects are strong when the adapting and probe expressions share…

Bathymetric surveys at highway bridges crossing the Missouri River in Kansas City, Missouri, using a multibeam echo sounder, 2010

Science.gov (United States)

Huizinga, Richard J.

2010-01-01

Bathymetric surveys were conducted by the U.S. Geological Survey, in cooperation with the Missouri Department of Transportation, on the Missouri River in the vicinity of nine bridges at seven highway crossings in Kansas City, Missouri, in March 2010. A multibeam echo sounder mapping system was used to obtain channel-bed elevations for river reaches that ranged from 1,640 to 1,800 feet long and extending from bank to bank in the main channel of the Missouri River. These bathymetric scans will be used by the Missouri Department of Transportation to assess the condition of the bridges for stability and integrity with respect to bridge scour. Bathymetric data were collected around every pier that was in water, except those at the edge of the water or in extremely shallow water, and one pier that was surrounded by a large debris raft. A scour hole was present at every pier for which bathymetric data could be obtained. The scour hole at a given pier varied in depth relative to the upstream channel bed, depending on the presence and proximity of other piers or structures upstream from the pier in question. The surveyed channel bed at the bottom of the scour hole was between 5 and 50 feet above bedrock. At bridges with drilled shaft foundations, generally there was exposure of the upstream end of the seal course and the seal course often was undermined to some extent. At one site, the minimum elevation of the scour hole at the main channel pier was about 10 feet below the bottom of the seal course, and the sides of the drilled shafts were evident in a point cloud visualization of the data at that pier. However, drilled shafts generally penetrated 20 feet into bedrock. Undermining of the seal course was evident as a sonic 'shadow' in the point cloud visualization of several of the piers. Large dune features were present in the channel at nearly all of the surveyed sites, as were numerous smaller dunes and many ripples. Several of the sites are on or near bends in the river

"Express yourself": culture and the effect of self-expression on choice.

Science.gov (United States)

Kim, Heejung S; Sherman, David K

2007-01-01

Whereas self-expression is valued in the United States, it is not privileged with such a cultural emphasis in East Asia. Four studies demonstrate the psychological implications of this cultural difference. Studies 1 and 2 found that European Americans value self-expression more than East Asians/East Asian Americans. Studies 3 and 4 examined the roles of expression in preference judgments. In Study 3, the expression of choice led European Americans but not East Asian Americans to be more invested in what they chose. Study 4 examined the connection between the value of expression and the effect of choice expression and showed that European Americans place greater emphasis on self-expression than East Asian Americans, and this difference explained the cultural difference in Study 3. This research highlights the importance of the cultural meanings of self-expression and the moderating role of cultural beliefs on the psychological effect of self-expression. 2007 APA, all rights reserved

Characterization of differentially expressed genes using high-dimensional co-expression networks

DEFF Research Database (Denmark)

Coelho Goncalves de Abreu, Gabriel; Labouriau, Rodrigo S.

2010-01-01

We present a technique to characterize differentially expressed genes in terms of their position in a high-dimensional co-expression network. The set-up of Gaussian graphical models is used to construct representations of the co-expression network in such a way that redundancy and the propagation...... that allow to make effective inference in problems with high degree of complexity (e.g. several thousands of genes) and small number of observations (e.g. 10-100) as typically occurs in high throughput gene expression studies. Taking advantage of the internal structure of decomposable graphical models, we...... construct a compact representation of the co-expression network that allows to identify the regions with high concentration of differentially expressed genes. It is argued that differentially expressed genes located in highly interconnected regions of the co-expression network are less informative than...

Facial Expressivity at 4 Months: A Context by Expression Analysis.

Science.gov (United States)

Bennett, David S; Bendersky, Margaret; Lewis, Michael

2002-01-01

The specificity predicted by differential emotions theory (DET) for early facial expressions in response to 5 different eliciting situations was studied in a sample of 4-month-old infants (n = 150). Infants were videotaped during tickle, sour taste, jack-in-the-box, arm restraint, and masked-stranger situations and their expressions were coded second by second. Infants showed a variety of facial expressions in each situation; however, more infants exhibited positive (joy and surprise) than negative expressions (anger, disgust, fear, and sadness) across all situations except sour taste. Consistent with DET-predicted specificity, joy expressions were the most common in response to tickling, and were less common in response to other situations. Surprise expressions were the most common in response to the jack-in-the-box, as predicted, but also were the most common in response to the arm restraint and masked-stranger situations, indicating a lack of specificity. No evidence of predicted specificity was found for anger, disgust, fear, and sadness expressions. Evidence of individual differences in expressivity within situations, as well as stability in the pattern across situations, underscores the need to examine both child and contextual factors in studying emotional development. The results provide little support for the DET postulate of situational specificity and suggest that a synthesis of differential emotions and dynamic systems theories of emotional expression should be considered.

Global expression differences and tissue specific expression differences in rice evolution result in two contrasting types of differentially expressed genes

KAUST Repository

Horiuchi, Youko

2015-12-23

Background Since the development of transcriptome analysis systems, many expression evolution studies characterized evolutionary forces acting on gene expression, without explicit discrimination between global expression differences and tissue specific expression differences. However, different types of gene expression alteration should have different effects on an organism, the evolutionary forces that act on them might be different, and different types of genes might show different types of differential expression between species. To confirm this, we studied differentially expressed (DE) genes among closely related groups that have extensive gene expression atlases, and clarified characteristics of different types of DE genes including the identification of regulating loci for differential expression using expression quantitative loci (eQTL) analysis data. Results We detected differentially expressed (DE) genes between rice subspecies in five homologous tissues that were verified using japonica and indica transcriptome atlases in public databases. Using the transcriptome atlases, we classified DE genes into two types, global DE genes and changed-tissues DE genes. Global type DE genes were not expressed in any tissues in the atlas of one subspecies, however changed-tissues type DE genes were expressed in both subspecies with different tissue specificity. For the five tissues in the two japonica-indica combinations, 4.6 ± 0.8 and 5.9 ± 1.5 % of highly expressed genes were global and changed-tissues DE genes, respectively. Changed-tissues DE genes varied in number between tissues, increasing linearly with the abundance of tissue specifically expressed genes in the tissue. Molecular evolution of global DE genes was rapid, unlike that of changed-tissues DE genes. Based on gene ontology, global and changed-tissues DE genes were different, having no common GO terms. Expression differences of most global DE genes were regulated by cis-eQTLs. Expression

Caricaturing facial expressions.

Science.gov (United States)

Calder, A J; Rowland, D; Young, A W; Nimmo-Smith, I; Keane, J; Perrett, D I

2000-08-14

The physical differences between facial expressions (e.g. fear) and a reference norm (e.g. a neutral expression) were altered to produce photographic-quality caricatures. In Experiment 1, participants rated caricatures of fear, happiness and sadness for their intensity of these three emotions; a second group of participants rated how 'face-like' the caricatures appeared. With increasing levels of exaggeration the caricatures were rated as more emotionally intense, but less 'face-like'. Experiment 2 demonstrated a similar relationship between emotional intensity and level of caricature for six different facial expressions. Experiments 3 and 4 compared intensity ratings of facial expression caricatures prepared relative to a selection of reference norms - a neutral expression, an average expression, or a different facial expression (e.g. anger caricatured relative to fear). Each norm produced a linear relationship between caricature and rated intensity of emotion; this finding is inconsistent with two-dimensional models of the perceptual representation of facial expression. An exemplar-based multidimensional model is proposed as an alternative account.

System for face recognition under expression variations of neutral-sampled individuals using recognized expression warping and a virtual expression-face database

Science.gov (United States)

Petpairote, Chayanut; Madarasmi, Suthep; Chamnongthai, Kosin

2018-01-01

The practical identification of individuals using facial recognition techniques requires the matching of faces with specific expressions to faces from a neutral face database. A method for facial recognition under varied expressions against neutral face samples of individuals via recognition of expression warping and the use of a virtual expression-face database is proposed. In this method, facial expressions are recognized and the input expression faces are classified into facial expression groups. To aid facial recognition, the virtual expression-face database is sorted into average facial-expression shapes and by coarse- and fine-featured facial textures. Wrinkle information is also employed in classification by using a process of masking to adjust input faces to match the expression-face database. We evaluate the performance of the proposed method using the CMU multi-PIE, Cohn-Kanade, and AR expression-face databases, and we find that it provides significantly improved results in terms of face recognition accuracy compared to conventional methods and is acceptable for facial recognition under expression variation.

Path Expressions

Science.gov (United States)

1975-06-01

Traditionally, synchronization of concurrent processes is coded in line by operations on semaphores or similar objects. Path expressions move the...discussion about a variety of synchronization primitives . An analysis of their relative power is found in [3]. Path expressions do not introduce yet...another synchronization primitive . A path expression relates to such primitives as a for- or while-statement of an ALGOL-like language relates to a JUMP

What emotion does the "facial expression of disgust" express?

Science.gov (United States)

Pochedly, Joseph T; Widen, Sherri C; Russell, James A

2012-12-01

The emotion attributed to the prototypical "facial expression of disgust" (a nose scrunch) depended on what facial expressions preceded it. In two studies, the majority of 120 children (5-14 years) and 135 adults (16-58 years) judged the nose scrunch as expressing disgust when the preceding set included an anger scowl, but as angry when the anger scowl was omitted. An even greater proportion of observers judged the nose scrunch as angry when the preceding set also included a facial expression of someone about to be sick. The emotion attributed to the nose scrunch therefore varies with experimental context. PsycINFO Database Record (c) 2012 APA, all rights reserved.

Expression modeling for expression-invariant face recognition

NARCIS (Netherlands)

Haar, F.B. Ter; Veltkamp, R.C.

2010-01-01

Morphable face models have proven to be an effective tool for 3D face modeling and face recognition, but the extension to 3D face scans with expressions is still a challenge. The two main difficulties are (1) how to build a new morphable face model that deals with expressions, and (2) how to fit

Enhancement and evaluation of an algorithm for atmospheric profiling continuity from Aqua to Suomi-NPP

Science.gov (United States)

Lipton, A.; Moncet, J. L.; Payne, V.; Lynch, R.; Polonsky, I. N.

2017-12-01

We will present recent results from an algorithm for producing climate-quality atmospheric profiling earth system data records (ESDRs) for application to data from hyperspectral sounding instruments, including the Atmospheric InfraRed Sounder (AIRS) on EOS Aqua and the Cross-track Infrared Sounder (CrIS) on Suomi-NPP, along with their companion microwave sounders, AMSU and ATMS, respectively. The ESDR algorithm uses an optimal estimation approach and the implementation has a flexible, modular software structure to support experimentation and collaboration. Data record continuity benefits from the fact that the same algorithm can be applied to different sensors, simply by providing suitable configuration and data files. Developments to be presented include the impact of a radiance-based pre-classification method for the atmospheric background. In addition to improving retrieval performance, pre-classification has the potential to reduce the sensitivity of the retrievals to the climatological data from which the background estimate and its error covariance are derived. We will also discuss evaluation of a method for mitigating the effect of clouds on the radiances, and enhancements of the radiative transfer forward model.

Gene Expression Commons: an open platform for absolute gene expression profiling.

Directory of Open Access Journals (Sweden)

Jun Seita

Full Text Available Gene expression profiling using microarrays has been limited to comparisons of gene expression between small numbers of samples within individual experiments. However, the unknown and variable sensitivities of each probeset have rendered the absolute expression of any given gene nearly impossible to estimate. We have overcome this limitation by using a very large number (>10,000 of varied microarray data as a common reference, so that statistical attributes of each probeset, such as the dynamic range and threshold between low and high expression, can be reliably discovered through meta-analysis. This strategy is implemented in a web-based platform named "Gene Expression Commons" (https://gexc.stanford.edu/ which contains data of 39 distinct highly purified mouse hematopoietic stem/progenitor/differentiated cell populations covering almost the entire hematopoietic system. Since the Gene Expression Commons is designed as an open platform, investigators can explore the expression level of any gene, search by expression patterns of interest, submit their own microarray data, and design their own working models representing biological relationship among samples.

Global expression differences and tissue specific expression differences in rice evolution result in two contrasting types of differentially expressed genes

KAUST Repository

Horiuchi, Youko; Harushima, Yoshiaki; Fujisawa, Hironori; Mochizuki, Takako; Fujita, Masahiro; Ohyanagi, Hajime; Kurata, Nori

2015-01-01

Since the development of transcriptome analysis systems, many expression evolution studies characterized evolutionary forces acting on gene expression, without explicit discrimination between global expression differences and tissue

After Effects expressions

CERN Document Server

Geduld, Marcus

2013-01-01

Put the power of Expressions to work in your animations with controls and efficiencies impossible to achieve with traditional keyframing techniques. No programming skills are required. Foundation concepts and skills orient the new designer and serve as a handy reference to the experienced one. Basics of creating expressions, variables, commands, and expression helpers precede the leap into javascript and math essentials for more advanced expressions that include randomness, physical simularions and 3D. Full color illustrations display the scripts and the resulti

Express web application development

CERN Document Server

Yaapa, Hage

2013-01-01

Express Web Application Development is a practical introduction to learning about Express. Each chapter introduces you to a different area of Express, using screenshots and examples to get you up and running as quickly as possible.If you are looking to use Express to build your next web application, ""Express Web Application Development"" will help you get started and take you right through to Express' advanced features. You will need to have an intermediate knowledge of JavaScript to get the most out of this book.

Simultaneous Breast Expression in Breastfeeding Women Is More Efficacious Than Sequential Breast Expression

Science.gov (United States)

Garbin, Catherine P.; Hartmann, Peter E.; Kent, Jacqueline C.

2012-01-01

Abstract Introduction Simultaneous (SIM) breast expression saves mothers time compared with sequential (SEQ) expression, but it remains unclear whether the two methods differ in milk output efficiency and efficacy. Subjects and Methods The Showmilk device (Medela AG, Baar, Switzerland) was used to measure milk output and milk ejection during breast expression (electric pump) in 31 Australian breastfeeding mothers of term infants (median age, 19 weeks [interquartile range, 10–33 weeks]). The order of expression type (SIM/SEQ) and breast (left/right) was randomized. Results SIM expression yielded more milk ejections (p≤0.001) and greater amounts of milk at 2, 5, and 10 minutes (p≤0.01) and removed a greater total amount of milk (p≤0.01) and percentage of available milk (p<0.05) than SEQ expression. After SIM expression the cream content of both the overall (8.3% [p≤0.05]) and postexpression (12.6% [p≤0.001]) milk were greater. During SEQ expression, the breast expressed first had a shorter time to 50% and 80% of the total amount of milk than the breast expressed second (p≤0.05), but, overall, a similar percentage of available milk was removed from both breasts. Conclusions SIM expression stimulated more milk ejections and was a more efficient and efficacious method of expression, yielding milk with a higher energy content. PMID:23039397

LocExpress: a web server for efficiently estimating expression of novel transcripts.

Science.gov (United States)

Hou, Mei; Tian, Feng; Jiang, Shuai; Kong, Lei; Yang, Dechang; Gao, Ge

2016-12-22

The temporal and spatial-specific expression pattern of a transcript in multiple tissues and cell types can indicate key clues about its function. While several gene atlas available online as pre-computed databases for known gene models, it's still challenging to get expression profile for previously uncharacterized (i.e. novel) transcripts efficiently. Here we developed LocExpress, a web server for efficiently estimating expression of novel transcripts across multiple tissues and cell types in human (20 normal tissues/cells types and 14 cell lines) as well as in mouse (24 normal tissues/cell types and nine cell lines). As a wrapper to RNA-Seq quantification algorithm, LocExpress efficiently reduces the time cost by making abundance estimation calls increasingly within the minimum spanning bundle region of input transcripts. For a given novel gene model, such local context-oriented strategy allows LocExpress to estimate its FPKMs in hundreds of samples within minutes on a standard Linux box, making an online web server possible. To the best of our knowledge, LocExpress is the only web server to provide nearly real-time expression estimation for novel transcripts in common tissues and cell types. The server is publicly available at http://loc-express.cbi.pku.edu.cn .

Regular expressions cookbook

CERN Document Server

Goyvaerts, Jan

2009-01-01

This cookbook provides more than 100 recipes to help you crunch data and manipulate text with regular expressions. Every programmer can find uses for regular expressions, but their power doesn't come worry-free. Even seasoned users often suffer from poor performance, false positives, false negatives, or perplexing bugs. Regular Expressions Cookbook offers step-by-step instructions for some of the most common tasks involving this tool, with recipes for C#, Java, JavaScript, Perl, PHP, Python, Ruby, and VB.NET. With this book, you will: Understand the basics of regular expressions through a

Supplementary Material for: Global expression differences and tissue specific expression differences in rice evolution result in two contrasting types of differentially expressed genes

KAUST Repository

Horiuchi, Youko; Harushima, Yoshiaki; Fujisawa, Hironori; Mochizuki, Takako; Fujita, Masahiro; Ohyanagi, Hajime; Kurata, Nori

2015-01-01

Abstract Background Since the development of transcriptome analysis systems, many expression evolution studies characterized evolutionary forces acting on gene expression, without explicit discrimination between global expression differences and tissue specific expression differences. However, different types of gene expression alteration should have different effects on an organism, the evolutionary forces that act on them might be different, and different types of genes might show different types of differential expression between species. To confirm this, we studied differentially expressed (DE) genes among closely related groups that have extensive gene expression atlases, and clarified characteristics of different types of DE genes including the identification of regulating loci for differential expression using expression quantitative loci (eQTL) analysis data. Results We detected differentially expressed (DE) genes between rice subspecies in five homologous tissues that were verified using japonica and indica transcriptome atlases in public databases. Using the transcriptome atlases, we classified DE genes into two types, global DE genes and changed-tissues DE genes. Global type DE genes were not expressed in any tissues in the atlas of one subspecies, however changed-tissues type DE genes were expressed in both subspecies with different tissue specificity. For the five tissues in the two japonica-indica combinations, 4.6 ± 0.8 and 5.9 ± 1.5 % of highly expressed genes were global and changed-tissues DE genes, respectively. Changed-tissues DE genes varied in number between tissues, increasing linearly with the abundance of tissue specifically expressed genes in the tissue. Molecular evolution of global DE genes was rapid, unlike that of changed-tissues DE genes. Based on gene ontology, global and changed-tissues DE genes were different, having no common GO terms. Expression differences of most global DE genes were regulated by cis

Expression-dependent susceptibility to face distortions in processing of facial expressions of emotion.

Science.gov (United States)

Guo, Kun; Soornack, Yoshi; Settle, Rebecca

2018-03-05

Our capability of recognizing facial expressions of emotion under different viewing conditions implies the existence of an invariant expression representation. As natural visual signals are often distorted and our perceptual strategy changes with external noise level, it is essential to understand how expression perception is susceptible to face distortion and whether the same facial cues are used to process high- and low-quality face images. We systematically manipulated face image resolution (experiment 1) and blur (experiment 2), and measured participants' expression categorization accuracy, perceived expression intensity and associated gaze patterns. Our analysis revealed a reasonable tolerance to face distortion in expression perception. Reducing image resolution up to 48 × 64 pixels or increasing image blur up to 15 cycles/image had little impact on expression assessment and associated gaze behaviour. Further distortion led to decreased expression categorization accuracy and intensity rating, increased reaction time and fixation duration, and stronger central fixation bias which was not driven by distortion-induced changes in local image saliency. Interestingly, the observed distortion effects were expression-dependent with less deterioration impact on happy and surprise expressions, suggesting this distortion-invariant facial expression perception might be achieved through the categorical model involving a non-linear configural combination of local facial features. Copyright © 2018 Elsevier Ltd. All rights reserved.

Matching faces with emotional expressions

Directory of Open Access Journals (Sweden)

Wenfeng eChen

2011-08-01

Full Text Available There is some evidence that faces with a happy expression are recognized better than faces with other expressions. However, little is known about whether this happy face advantage also applies to perceptual face matching, and whether similar differences exist among other expressions. Using a sequential matching paradigm, we systematically compared the effects of seven basic facial expressions on identity recognition. Identity matching was quickest when a pair of faces had an identical happy/sad/neutral expression, poorer when they had a fearful/surprise/angry expression, and poorest when they had a disgust expression. Faces with a happy/sad/fear/surprise expression were matched faster than those with an anger/disgust expression when the second face in a pair had a neutral expression. These results demonstrate that effects of facial expression on identity recognition are not limited to happy faces when a learned face is immediately tested. The results suggest different influences of expression in perceptual matching and long-term recognition memory.

The MPI facial expression database--a validated database of emotional and conversational facial expressions.

Directory of Open Access Journals (Sweden)

Kathrin Kaulard

Full Text Available The ability to communicate is one of the core aspects of human life. For this, we use not only verbal but also nonverbal signals of remarkable complexity. Among the latter, facial expressions belong to the most important information channels. Despite the large variety of facial expressions we use in daily life, research on facial expressions has so far mostly focused on the emotional aspect. Consequently, most databases of facial expressions available to the research community also include only emotional expressions, neglecting the largely unexplored aspect of conversational expressions. To fill this gap, we present the MPI facial expression database, which contains a large variety of natural emotional and conversational expressions. The database contains 55 different facial expressions performed by 19 German participants. Expressions were elicited with the help of a method-acting protocol, which guarantees both well-defined and natural facial expressions. The method-acting protocol was based on every-day scenarios, which are used to define the necessary context information for each expression. All facial expressions are available in three repetitions, in two intensities, as well as from three different camera angles. A detailed frame annotation is provided, from which a dynamic and a static version of the database have been created. In addition to describing the database in detail, we also present the results of an experiment with two conditions that serve to validate the context scenarios as well as the naturalness and recognizability of the video sequences. Our results provide clear evidence that conversational expressions can be recognized surprisingly well from visual information alone. The MPI facial expression database will enable researchers from different research fields (including the perceptual and cognitive sciences, but also affective computing, as well as computer vision to investigate the processing of a wider range of natural

Principle Component Analysis of AIRS and CrIS Data

Science.gov (United States)

Aumann, H. H.; Manning, Evan

2015-01-01

Synthetic Eigen Vectors (EV) used for the statistical analysis of the PC reconstruction residual of large ensembles of data are a novel tool for the analysis of data from hyperspectral infrared sounders like the Atmospheric Infrared Sounder (AIRS) on the EOS Aqua and the Cross-track Infrared Sounder (CrIS) on the SUOMI polar orbiting satellites. Unlike empirical EV, which are derived from the observed spectra, the synthetic EV are derived from a large ensemble of spectra which are calculated assuming that, given a state of the atmosphere, the spectra created by the instrument can be accurately calculated. The synthetic EV are then used to reconstruct the observed spectra. The analysis of the differences between the observed spectra and the reconstructed spectra for Simultaneous Nadir Overpasses of tropical oceans reveals unexpected differences at the more than 200 mK level under relatively clear conditions, particularly in the mid-wave water vapor channels of CrIS. The repeatability of these differences using independently trained SEV and results from different years appears to rule out inconsistencies in the radiative transfer algorithm or the data simulation. The reasons for these discrepancies are under evaluation.

Acoustic detection of momentum transfer during the abrupt transition from a laminar to a turbulent atmospheric boundary layer1

International Nuclear Information System (INIS)

Schubert, J.F.

1977-01-01

Acoustic sounder measurements of a vertical profile of the abrupt transition from a laminar to a turbulent atmospheric boundary layer were compared with meteorological measurements made at 10 and 137 m on an instrumented tower. Sounder data show that conditions necessary for the onset of the momentum burst phenomenon exist sometime during a clear afternoon when heat flux changes sign and the planetary surface cools. Under these conditions, the lowest part of the atmospheric boundary layer becomes stable. Prior to this situation, the entire boundary layer is in turbulent motion from surface heating. The boundary layer is then an effective barrier for all fluxes, and as the maximum flux Richardson number is reached at some height close to but above the surface, turbulence is dampened and a laminar layer forms. The profile of this layer is recorded by the sounder. Surface temperature drops, a strong wind shear develops, and the Richardson number decreases below its critical value (Ri/sub cr/<0.25). Subsequently, the laminar layer is eroded by turbulence from above, and with a burst of momentum and heat, it eventually reaches the ground

Developing the science product algorithm testbed for Chinese next-generation geostationary meteorological satellites: Fengyun-4 series

Science.gov (United States)

Min, Min; Wu, Chunqiang; Li, Chuan; Liu, Hui; Xu, Na; Wu, Xiao; Chen, Lin; Wang, Fu; Sun, Fenglin; Qin, Danyu; Wang, Xi; Li, Bo; Zheng, Zhaojun; Cao, Guangzhen; Dong, Lixin

2017-08-01

Fengyun-4A (FY-4A), the first of the Chinese next-generation geostationary meteorological satellites, launched in 2016, offers several advances over the FY-2: more spectral bands, faster imaging, and infrared hyperspectral measurements. To support the major objective of developing the prototypes of FY-4 science algorithms, two science product algorithm testbeds for imagers and sounders have been developed by the scientists in the FY-4 Algorithm Working Group (AWG). Both testbeds, written in FORTRAN and C programming languages for Linux or UNIX systems, have been tested successfully by using Intel/g compilers. Some important FY-4 science products, including cloud mask, cloud properties, and temperature profiles, have been retrieved successfully through using a proxy imager, Himawari-8/Advanced Himawari Imager (AHI), and sounder data, obtained from the Atmospheric InfraRed Sounder, thus demonstrating their robustness. In addition, in early 2016, the FY-4 AWG was developed based on the imager testbed—a near real-time processing system for Himawari-8/AHI data for use by Chinese weather forecasters. Consequently, robust and flexible science product algorithm testbeds have provided essential and productive tools for popularizing FY-4 data and developing substantial improvements in FY-4 products.

Identification of Emotional Facial Expressions: Effects of Expression, Intensity, and Sex on Eye Gaze.

Directory of Open Access Journals (Sweden)

Laura Jean Wells

Full Text Available The identification of emotional expressions is vital for social interaction, and can be affected by various factors, including the expressed emotion, the intensity of the expression, the sex of the face, and the gender of the observer. This study investigates how these factors affect the speed and accuracy of expression recognition, as well as dwell time on the two most significant areas of the face: the eyes and the mouth. Participants were asked to identify expressions from female and male faces displaying six expressions (anger, disgust, fear, happiness, sadness, and surprise, each with three levels of intensity (low, moderate, and normal. Overall, responses were fastest and most accurate for happy expressions, but slowest and least accurate for fearful expressions. More intense expressions were also classified most accurately. Reaction time showed a different pattern, with slowest response times recorded for expressions of moderate intensity. Overall, responses were slowest, but also most accurate, for female faces. Relative to male observers, women showed greater accuracy and speed when recognizing female expressions. Dwell time analyses revealed that attention to the eyes was about three times greater than on the mouth, with fearful eyes in particular attracting longer dwell times. The mouth region was attended to the most for fearful, angry, and disgusted expressions and least for surprise. These results extend upon previous findings to show important effects of expression, emotion intensity, and sex on expression recognition and gaze behaviour, and may have implications for understanding the ways in which emotion recognition abilities break down.

Identification of Emotional Facial Expressions: Effects of Expression, Intensity, and Sex on Eye Gaze.

Science.gov (United States)

Wells, Laura Jean; Gillespie, Steven Mark; Rotshtein, Pia

2016-01-01

The identification of emotional expressions is vital for social interaction, and can be affected by various factors, including the expressed emotion, the intensity of the expression, the sex of the face, and the gender of the observer. This study investigates how these factors affect the speed and accuracy of expression recognition, as well as dwell time on the two most significant areas of the face: the eyes and the mouth. Participants were asked to identify expressions from female and male faces displaying six expressions (anger, disgust, fear, happiness, sadness, and surprise), each with three levels of intensity (low, moderate, and normal). Overall, responses were fastest and most accurate for happy expressions, but slowest and least accurate for fearful expressions. More intense expressions were also classified most accurately. Reaction time showed a different pattern, with slowest response times recorded for expressions of moderate intensity. Overall, responses were slowest, but also most accurate, for female faces. Relative to male observers, women showed greater accuracy and speed when recognizing female expressions. Dwell time analyses revealed that attention to the eyes was about three times greater than on the mouth, with fearful eyes in particular attracting longer dwell times. The mouth region was attended to the most for fearful, angry, and disgusted expressions and least for surprise. These results extend upon previous findings to show important effects of expression, emotion intensity, and sex on expression recognition and gaze behaviour, and may have implications for understanding the ways in which emotion recognition abilities break down.

EXPRESS Service to the International Space Station: EXPRESS Pallet

Science.gov (United States)

Primm, Lowell; Bergmann, Alan

1998-01-01

The International Space Station (ISS) will be the ultimate scientific accomplishment in the history of NASA, with its primary objective of providing unique scientific investigation opportunities. This objective is the basis for the creation of the EXPRESS Pallet System (ExPs). The EXPRESS Pallet will provide extremal/unpressurized accommodations for a wide variety of external users. The payload developers represent many science disciplines, including earth observation, communications, solar and deep space viewing, long-term exposure, and many others. The EXPRESS Pallet will provide a mechanism to maximum utilization of the limited ISS unpressurized payload volume, standard physical payload interfaces for users, a standard integration template for users and the capability to changeout payloads on-orbit. The EXPRESS Pallet provides access to Ram, Wake, Starboard, Port, Nadir, Zenith and Earth Limb for exposure and viewing. 'Me ExPs consists of the Pallet structure, payload Adapters, and a subsystem assembly which includes data controller, power distribution and conversion, and Extra Vehicular Robotics/Extra-Vehicular Activity systems.

Neuroglobin over expressing mice

DEFF Research Database (Denmark)

Raida, Zindy; Hundahl, Christian Ansgar; Nyengaard, Jens R

2013-01-01

showed over expression to be confined to primarily the cortex, hippocampus, cerebellum, and only in neurons. The level and expression pattern of endogenous Neuroglobin was unaffected by insertion of the over expressing Ngb transgene. Neuroglobin over expression resulted in a significant reduction...... previous reports, Neuroglobin over expression is not global but confined to a few well-defined brain regions, and only in neurons. This study confirms previous reports showing a correlation between reduced infarct volume and elevated Neuroglobin levels, but underlines the need to study the likely...

Expressive means of irony expression in Marcel Proust’s aphorisms

Directory of Open Access Journals (Sweden)

Shumakova Alla Petrovna

2016-06-01

Full Text Available The relevance of the study is determined by the lack of work on the analysis of linguistic expressive means of aphorisms by Marcel Proust. The material of the research are aphoristic sayings of ironic character found in the novels of Proust “Jean Santeuil”, “La Prisonnière”, “Albertine disparue”, “A l’ombre des jeunes filles en fleurs”, “Sodome et Gomorrhe”, “Le Côté de Guermantes”, “Le Temps retrouvé”. Since none of the linguistic resources of imagery is self-sufficient it is considered the most effective for the expression of irony combination means expressive syntax (figures and figurative means (tropes. Among the means of expressive syntax it is analyzed the use of aphorisms by Proust, like parallel structures, chiasm, antithesis, repetition, poliptota. The most frequent in aphorisms by Proust are metaphors and similes. An important aspect of the study of aphorisms of Marcel Proust is a detailed examination of the arsenal of linguistic means of language pun used to create an ironic effect. The material of the pun became a unit of lexical and syntactic level, forming a paradox, pun, parody and illogic. Through the use of expressive means of expression of irony and their various combinations aphorisms Proust acquired ambiguity, originality, intellectual and emotional capacity.

Results of NASA/NOAA HES Trade Studies

Science.gov (United States)

Susskind, Joel

2011-01-01

This slide presentation reviews the trade studies that were done for the Hyperspectral Environmental Suite (HES). The goal of the trade studies was to minimize instrument cost and risk while producing scientifically useful products. Three vendors were selected to perform the trade study, and were to conduct 11 studies, with the first study a complete wish list of things that scientists would like from GEO orbit to the 11th study which was for a Reduced Accommodation Sounder (RAS) which would still result in useful scientific products, within constrains compatible with flight on GEOS-R. The RAS's from each vendor and one other HES sounders designs are reviewed.

Investigating the possibility of the CONSERT instrument operating as a bi-static RADAR sounder during the seperation, descent and landing phase of the ROSETTA mission

Science.gov (United States)

Statz, C.; Hegler, S.; Plettemeier, D.; Berquin, Y. P.; Herique, A.; Kofman, W. W.

2012-12-01

The main scientific objective of the Comet Nucleus Sounding Experiment by Radiowave Transmission (CONSERT) is to determine the dielectric properties of comet 67P/Chuyurmov-Gerasimenko's nucleus. This will be achieved by performing a sounding of the comet's core between the lander "Philae" launched on the comet's surface and the orbiter "Rosetta". For the sounding the lander will receive, process and retransmit the radio signal emitted by the CONSERT instrument aboard the orbiter. With data measured during the first science phase, a three-dimensional model of the material distribution with regard to the complex dielectric permittivity of the comet's nucleus is to be reconstructed. In order to increase the scientific outcome of the experiment and to collect data beneficial for the main scientific objective, it may be considered to operate the CONSERT instrument as a bi-static RADAR sounder during the non mission-critical parts of the separation, descent and landing (SDL) phase, i.e. when the lander is launched onto the comet's surface, of the ROSETTA mission. The data measured during this phase will be mainly echoes from the comet's surface and first meters of subsurface. Based on this data, we intent to create an initial dielectric permittivity mapping of the comet's surface at and around the landing site In order to estimate the performance of the instrument in this special operational mode, simulations of a sounding in SDL configuration were performed. The simulations are based on a hybrid method-of-moments physical-optics (EFIE-DPO) approach for large dielectric bodies with consideration of the behavior of the instrument's antennas and coupling with the spacecraft as well as polarization effects. The simulated results are furthermore processed in a system-level-instrument-simulator to include effects such as a realistic sounding signal, pulse-compression and analog digital conversion in the estimation of the sounding capabilities. The main objective of the

The expressive stance: intentionality, expression, and machine art

OpenAIRE

Linson, Adam

2013-01-01

This paper proposes a new interpretive stance for interpreting artistic works and performances that is relevant to artificial intelligence research but also has broader implications. Termed the expressive stance, this stance makes intelligible a critical distinction between present-day machine art and human art, but allows for the possibility that future machine art could find a place alongside our own. The expressive stance is elaborated as a response to Daniel Dennett's notion of the intent...

The MPI Facial Expression Database — A Validated Database of Emotional and Conversational Facial Expressions

Science.gov (United States)

Kaulard, Kathrin; Cunningham, Douglas W.; Bülthoff, Heinrich H.; Wallraven, Christian

2012-01-01

The ability to communicate is one of the core aspects of human life. For this, we use not only verbal but also nonverbal signals of remarkable complexity. Among the latter, facial expressions belong to the most important information channels. Despite the large variety of facial expressions we use in daily life, research on facial expressions has so far mostly focused on the emotional aspect. Consequently, most databases of facial expressions available to the research community also include only emotional expressions, neglecting the largely unexplored aspect of conversational expressions. To fill this gap, we present the MPI facial expression database, which contains a large variety of natural emotional and conversational expressions. The database contains 55 different facial expressions performed by 19 German participants. Expressions were elicited with the help of a method-acting protocol, which guarantees both well-defined and natural facial expressions. The method-acting protocol was based on every-day scenarios, which are used to define the necessary context information for each expression. All facial expressions are available in three repetitions, in two intensities, as well as from three different camera angles. A detailed frame annotation is provided, from which a dynamic and a static version of the database have been created. In addition to describing the database in detail, we also present the results of an experiment with two conditions that serve to validate the context scenarios as well as the naturalness and recognizability of the video sequences. Our results provide clear evidence that conversational expressions can be recognized surprisingly well from visual information alone. The MPI facial expression database will enable researchers from different research fields (including the perceptual and cognitive sciences, but also affective computing, as well as computer vision) to investigate the processing of a wider range of natural facial expressions

Expression of Na,K-ATPase and H,K-ATPase Isoforms with the Baculovirus Expression System

NARCIS (Netherlands)

Koenderink, J.B.; Swarts, H.G.

2016-01-01

P-type ATPases can be expressed in several cell systems. The baculovirus expressions system uses an insect virus to enter and express proteins in Sf9 insect cells. This expression system is a lytic system in which the cells will die a few days after viral infection. Subsequently, the expressed

Effects of Expressive Writing on Psychological and Physical Health: The Moderating Role of Emotional Expressivity

Science.gov (United States)

Haltom, Kate E.; Mulvenna, Catherine M.; Lieberman, Matthew D.; Stanton, Annette L.

2013-01-01

The current study assessed main effects and moderators (including emotional expressiveness, emotional processing and ambivalence over emotional expression) of the effects of expressive writing in a sample of healthy adults. Young adult participants (N = 116) were randomly assigned to write for 20 minutes on four occasions about deepest thoughts and feelings regarding their most stressful/traumatic event in the past five years (expressive writing) or about a control topic (control). Dependent variables were indicators of anxiety, depression, and physical symptoms. No significant effects of writing condition were evident on anxiety, depressive symptoms, or physical symptoms. Emotional expressiveness emerged as a significant moderator of anxiety outcomes, however. Within the expressive writing group, participants high in expressiveness evidenced a significant reduction in anxiety at three-month follow-up, and participants low in expressiveness showed a significant increase in anxiety. Expressiveness did not predict change in anxiety in the control group. These findings on anxiety are consistent with the matching hypothesis, which suggests that matching a person’s naturally elected coping approach with an assigned intervention is beneficial. These findings also suggest that expressive writing about a stressful event may be contraindicated for individuals who do not typically express emotions. PMID:23742666

Characterization and Oral Delivery of Proinsulin-Transferrin Fusion Protein Expressed Using ExpressTec

Directory of Open Access Journals (Sweden)

Yu-Sheng Chen

2018-01-01

Full Text Available Proinsulin-transferrin fusion protein (ProINS-Tf has been designed and successfully expressed from the mammalian HEK293 cells (HEK-ProINS-Tf. It was found that HEK-ProINS-Tf could be converted into an activated form in the liver. Furthermore, HEK-ProINS-Tf was demonstrated as an extra-long acting insulin analogue with liver-specific insulin action in streptozotocin (STZ-induced type 1 diabetic mice. However, due to the low production yield from transfected HEK293 cells, there are other interesting features, including the oral bioavailability, which have not been fully explored and characterized. To improve the protein production yield, an alternative protein expression system, ExpressTec using transgenic rice (Oryza sativa L., was used. The intact and active rice-derived ProINS-Tf (ExpressTec-ProINS-Tf was successfully expressed from the transgenic rice expression system. Our results suggested that, although the insulin-like bioactivity of ExpressTec-ProINS-Tf was slightly lower in vitro, its potency of in vivo blood glucose control was considerably stronger than that of HEK-ProINS-Tf. The oral delivery studies in type 1 diabetic mice demonstrated a prolonged control of blood glucose to near-normal levels after oral administration of ExpressTec-ProINS-Tf. Results in this report suggest that ExpressTec-ProINS-Tf is a promising insulin analog with advantages including low cost, prolonged and liver targeting effects, and most importantly, oral bioactivity.

Determining Physical Mechanisms of Gene Expression Regulation from Single Cell Gene Expression Data

OpenAIRE

Ezer, Daphne; Moignard, Victoria; G?ttgens, Berthold; Adryan, Boris

2016-01-01

Many genes are expressed in bursts, which can contribute to cell-to-cell heterogeneity. It is now possible to measure this heterogeneity with high throughput single cell gene expression assays (single cell qPCR and RNA-seq). These experimental approaches generate gene expression distributions which can be used to estimate the kinetic parameters of gene expression bursting, namely the rate that genes turn on, the rate that genes turn off, and the rate of transcription. We construct a complete ...

Cyclooxygenase-2 expression in the normal human eye and its expression pattern in selected eye tumours

DEFF Research Database (Denmark)

Wang, Jinmei; Wu, Yazhen; Heegaard, Steffen

2011-01-01

Purpose: Cyclooxygenase-2 (COX-2) is an enzyme involved in neoplastic processes. The purpose of the present study is to investigate COX-2 expression in the normal human eye and the expression pattern in selected eye tumours involving COX-2 expressing cells. Methods: Immunohistochemical staining...... using antibodies against COX-2 was performed on paraffin sections of normal human eyes and selected eye tumours arising from cells expressing COX-2. Results: Cyclooxygenase-2 expression was found in various structures of the normal eye. Abundant expression was seen in the cornea, iris, ciliary body...... and retina. The COX-2 expression was less in tumours deriving from the ciliary epithelium and also in retinoblastoma. Conclusion: Cyclooxygenase-2 is constitutively expressed in normal human eyes. The expression of COX-2 is much lower in selected eye tumours involving COX-2 expressing cells....

The predictive nature of transcript expression levels on protein expression in adult human brain.

Science.gov (United States)

Bauernfeind, Amy L; Babbitt, Courtney C

2017-04-24

Next generation sequencing methods are the gold standard for evaluating expression of the transcriptome. When determining the biological implications of such studies, the assumption is often made that transcript expression levels correspond to protein levels in a meaningful way. However, the strength of the overall correlation between transcript and protein expression is inconsistent, particularly in brain samples. Following high-throughput transcriptomic (RNA-Seq) and proteomic (liquid chromatography coupled with tandem mass spectrometry) analyses of adult human brain samples, we compared the correlation in the expression of transcripts and proteins that support various biological processes, molecular functions, and that are located in different areas of the cell. Although most categories of transcripts have extremely weak predictive value for the expression of their associated proteins (R 2 values of < 10%), transcripts coding for protein kinases and membrane-associated proteins, including those that are part of receptors or ion transporters, are among those that are most predictive of downstream protein expression levels. The predictive value of transcript expression for corresponding proteins is variable in human brain samples, reflecting the complex regulation of protein expression. However, we found that transcriptomic analyses are appropriate for assessing the expression levels of certain classes of proteins, including those that modify proteins, such as kinases and phosphatases, regulate metabolic and synaptic activity, or are associated with a cellular membrane. These findings can be used to guide the interpretation of gene expression results from primate brain samples.

Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System

Directory of Open Access Journals (Sweden)

Sina Mirzaahmadi

2011-01-01

Full Text Available The variety of recombinant protein expression systems have been developed as a resource of FVII gene expression. In the current study, the authors used a novel protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host for expression of FVII. Plasmid containing cDNA encoding full-length human FVII was introduced into Lizard Leishmania and positive transfectants were analyzed by SDS-PAGE and Western blot analysis. Furthermore, biological activity of purified protein was detected by PT assay. The recombinant strain harboring a construct was analyzed for expression of FVII at the mRNA and protein level. Purified rFVII was obtained and in order to confirm the purified compound was in fact rFVII. Western blot analysis was carried out. Clotting time in PT assay was reduced about 30 seconds with the purified rFVII. In Conclusion, this study has demonstrated, for the first time, that Leishmania cells can be used as an expression system for producing recombinant FVII.

Gene expression

International Nuclear Information System (INIS)

Hildebrand, C.E.; Crawford, B.D.; Walters, R.A.; Enger, M.D.

1983-01-01

We prepared probes for isolating functional pieces of the metallothionein locus. The probes enabled a variety of experiments, eventually revealing two mechanisms for metallothionein gene expression, the order of the DNA coding units at the locus, and the location of the gene site in its chromosome. Once the switch regulating metallothionein synthesis was located, it could be joined by recombinant DNA methods to other, unrelated genes, then reintroduced into cells by gene-transfer techniques. The expression of these recombinant genes could then be induced by exposing the cells to Zn 2+ or Cd 2+ . We would thus take advantage of the clearly defined switching properties of the metallothionein gene to manipulate the expression of other, perhaps normally constitutive, genes. Already, despite an incomplete understanding of how the regulatory switch of the metallothionein locus operates, such experiments have been performed successfully

SAFARI 2000 TOVS Surface and Atmospheric Parameters, 1-Deg, 1999-2001

Data.gov (United States)

National Aeronautics and Space Administration — ABSTRACT: NOAA's TIROS (Television Infrared Observation Satellite) Operational Vertical Sounder (TOVS) is a suite of three sensors: the Microwave Sounding Unit...

Regular Expression Pocket Reference

CERN Document Server

Stubblebine, Tony

2007-01-01

This handy little book offers programmers a complete overview of the syntax and semantics of regular expressions that are at the heart of every text-processing application. Ideal as a quick reference, Regular Expression Pocket Reference covers the regular expression APIs for Perl 5.8, Ruby (including some upcoming 1.9 features), Java, PHP, .NET and C#, Python, vi, JavaScript, and the PCRE regular expression libraries. This concise and easy-to-use reference puts a very powerful tool for manipulating text and data right at your fingertips. Composed of a mixture of symbols and text, regular exp

Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae.

Science.gov (United States)

Ishii, Jun; Kondo, Takashi; Makino, Harumi; Ogura, Akira; Matsuda, Fumio; Kondo, Akihiko

2014-05-01

Yeast has the potential to be used in bulk-scale fermentative production of fuels and chemicals due to its tolerance for low pH and robustness for autolysis. However, expression of multiple external genes in one host yeast strain is considerably labor-intensive due to the lack of polycistronic transcription. To promote the metabolic engineering of yeast, we generated systematic and convenient genetic engineering tools to express multiple genes in Saccharomyces cerevisiae. We constructed a series of multi-copy and integration vector sets for concurrently expressing two or three genes in S. cerevisiae by embedding three classical promoters. The comparative expression capabilities of the constructed vectors were monitored with green fluorescent protein, and the concurrent expression of genes was monitored with three different fluorescent proteins. Our multiple gene expression tool will be helpful to the advanced construction of genetically engineered yeast strains in a variety of research fields other than metabolic engineering. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

MLS/Aura Level 2 Diagnostics, Geophysical Parameter Grid V004

Data.gov (United States)

National Aeronautics and Space Administration — ML2DGG is the EOS Aura Microwave Limb Sounder (MLS) product containing geophysical diagnostic quantities pertaining directly to the standard geophysical data...

AIRS/Aqua Level 2 Cloud-cleared infrared radiances (AIRS+AMSU) V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 1B HSB geolocated and calibrated brightness temperatures V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 1B Visible/Near Infrared (VIS/NIR) quality assurance subset V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

Aqua AIRS Near Real Time (NRT) Level 2 Standard Physical Retrieval (AIRS+AMSU) V006

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

Aqua AIRS Level 2 Cloud-Cleared Infrared Radiances (AIRS+AMSU) V006

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Near Real Time (NRT) Level 1B AMSU (A1/A2) geolocated and calibrated brightness temperatures V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 2G Precipitation Estimate V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 1B Visible/Near Infrared (VIS/NIR) geolocated and calibrated radiances V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

Aqua AIRS Level 2 Near Real Time (NRT) Cloud-Cleared Infrared Radiances (AIRS+AMSU) V006

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 2 Support retrieval (AIRS+AMSU) V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

Aqua AIRS Level 2G Precipitation Estimate (AIRS+AMSU) V006

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 2 CO2 support retrieval (AIRS-only) V005 (AIRS2SPC) at GES DISC) V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

Aqua AIRS Level 2 Support Retrieval (AIRS+AMSU) V006

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

SAFARI 2000 TOVS Surface and Atmospheric Parameters, 1-Deg, 1999-2001

Data.gov (United States)

National Aeronautics and Space Administration — NOAA's TIROS (Television Infrared Observation Satellite) Operational Vertical Sounder (TOVS) is a suite of three sensors: the Microwave Sounding Unit (MSU), the High...

AIRS/Aqua Near Real Time (NRT) Level 1B Visible/Near Infrared (VIS/NIR) geolocated and calibrated radiances V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Near Real Time (NRT) Level 1B Infrared (IR) quality assurance subset V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 1B AMSU (A1/A2) geolocated and calibrated brightness temperatures V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 2 Standard physical retrieval (AIRS+AMSU) V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Level 1B Infrared (IR) quality assurance subset V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

AIRS/Aqua Near Real Time (NRT) Level 1B Visible/Near Infrared (VIS/NIR) quality assurance subset V005

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

Aqua AIRS Level 2 Standard Physical Retrieval (AIRS+AMSU) V006

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

Aqua AIRS Near Real Time (NRT) Level 2 Support Retrieval (AIRS+AMSU) V006

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a facility instrument aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In combination...

GLA MONTHLY GRIDS from NOAA-11 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA 5 DAY GRIDS from NOAA-9 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA DAILY GRIDS from NOAA-10 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA MONTHLY GRIDS from NOAA-9 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA 5 DAY GRIDS from NOAA-11 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA MONTHLY GRIDS from NOAA-10 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA DAILY GRIDS from NOAA-11 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA MONTHLY GRIDS from TIROSN V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA DAILY GRIDS from NOAA-12 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA 5 DAY GRIDS from NOAA-10 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

GLA DAILY GRIDS from NOAA-9 V001

Data.gov (United States)

National Aeronautics and Space Administration — This dataset contains the TIROS Operational Vertical Sounder (TOVS) level 3 geophysical parameters derived using the physical retrieval method of Susskind et al....

Morphing between expressions dissociates continuous from categorical representations of facial expression in the human brain

Science.gov (United States)

Harris, Richard J.; Young, Andrew W.; Andrews, Timothy J.

2012-01-01

Whether the brain represents facial expressions as perceptual continua or as emotion categories remains controversial. Here, we measured the neural response to morphed images to directly address how facial expressions of emotion are represented in the brain. We found that face-selective regions in the posterior superior temporal sulcus and the amygdala responded selectively to changes in facial expression, independent of changes in identity. We then asked whether the responses in these regions reflected categorical or continuous neural representations of facial expression. Participants viewed images from continua generated by morphing between faces posing different expressions such that the expression could be the same, could involve a physical change but convey the same emotion, or could differ by the same physical amount but be perceived as two different emotions. We found that the posterior superior temporal sulcus was equally sensitive to all changes in facial expression, consistent with a continuous representation. In contrast, the amygdala was only sensitive to changes in expression that altered the perceived emotion, demonstrating a more categorical representation. These results offer a resolution to the controversy about how facial expression is processed in the brain by showing that both continuous and categorical representations underlie our ability to extract this important social cue. PMID:23213218

Nestin expression in neuroepithelial tumors.

Science.gov (United States)

Schiffer, Davide; Manazza, Andrea; Tamagno, Ilaria

2006-05-29

Nestin is a marker of early stages of neurocytogenesis. It has been studied in 50 neuroepithelial tumors, mostly gliomas of different malignancy grades, by immunohistochemistry, immunofluorescence, immunoblotting, and confocal microscopy and compared with GFAP and Vimentin. As an early marker of differentiation, Nestin is almost not expressed in diffuse astrocytomas, variably expressed in anaplastic astrocytomas and strongly and irregularly expressed in glioblastomas. Negative in oligodendrogliomas, it stains ependymomas and shows a gradient of expression in pilocytic astrocytomas. In glioblastomas, Nestin distribution does not completely correspond to that of GFAP and Vimentin with which its expression varies in tumor cells in a complementary way, as confirmed by confocal microscopy. Tumor cells can thus either derive from or differentiate toward the neurocytogenetic stages. Hypothetically, they could be put in relation with radial glia where during embriogenesis the three antigens are successively expressed. Completely negative cells of invasive or recurrent glioblastomas may represent malignant selected clones after accumulation of mutations or early stem cells not expressing antigens.

FARO server: Meta-analysis of gene expression by matching gene expression signatures to a compendium of public gene expression data

DEFF Research Database (Denmark)

Manijak, Mieszko P.; Nielsen, Henrik Bjørn

2011-01-01

circumvented by instead matching gene expression signatures to signatures of other experiments. FINDINGS: To facilitate this we present the Functional Association Response by Overlap (FARO) server, that match input signatures to a compendium of 242 gene expression signatures, extracted from more than 1700...... Arabidopsis microarray experiments. CONCLUSIONS: Hereby we present a publicly available tool for robust characterization of Arabidopsis gene expression experiments which can point to similar experimental factors in other experiments. The server is available at http://www.cbs.dtu.dk/services/faro/....

Freedom of Expression.

Science.gov (United States)

Update on Law-Related Education, 1988

1988-01-01

Presents an activity which uses hypothetical situations to explore the proper boundaries of freedom of expression and the role of the U.S. Supreme Court in interpreting its limits. Appropriate for grades 4-12, the lesson includes such topics as the "clear and present danger" clause, student expression, obscenity, and defamation. (GEA)

AIRS/Aqua L1B Infrared (IR) geolocated and calibrated radiances V005 (AIRIBRAD) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a grating spectrometer (R = 1200) aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In...

AIRS/Aqua L3 Daily Standard Physical Retrieval (AIRS+AMSU) 1 degree x 1 degree V006 (AIRX3STD) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a grating spectrometer (R = 1200) aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In...

AIRS/Aqua L3 Monthly Quantization in Physical Units (AIRS+AMSU) 5 degrees x 5 degrees V006 (AIRX3QPM) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a grating spectrometer (R = 1200) aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In...

Low-power Cross-Correlator ASIC, Phase II

Data.gov (United States)

National Aeronautics and Space Administration — The NASA's PATH mission includes the GeoSTAR satellite that carries aboard a microwave sounder employing an array of 375 microwave antennas with corresponding...

AIRS/Aqua L1C Infrared (IR) resampled and corrected radiances V006 (AIRICRAD) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) is a grating spectrometer (R = 1200) aboard the second Earth Observing System (EOS) polar-orbiting platform, EOS Aqua. In...

P2-35: The KU Facial Expression Database: A Validated Database of Emotional and Conversational Expressions

Directory of Open Access Journals (Sweden)

Haenah Lee

2012-10-01

Full Text Available Facial expressions are one of the most important means of nonverbal communication transporting both emotional and conversational content. For investigating this large space of expressions we recently developed a large database containing dynamic emotional and conversational expressions in Germany (MPI facial expression database. As facial expressions crucially depend on the cultural context, however, a similar resource is needed for studies outside of Germany. Here, we introduce and validate a new, extensive Korean facial expression database containing dynamic emotional and conversational information. Ten individuals performed 62 expressions following a method-acting protocol, in which each person was asked to imagine themselves in one of 62 corresponding everyday scenarios and to react accordingly. To validate this database, we conducted two experiments: 20 participants were asked to name the appropriate expression for each of the 62 everyday scenarios shown as text. Ten additional participants were asked to name each of the 62 expression videos from 10 actors in addition to rating its naturalness. All naming answers were then rated as valid or invalid. Scenario validation yielded 89% valid answers showing that the scenarios are effective in eliciting appropriate expressions. Video sequences were judged as natural with an average of 66% valid answers. This is an excellent result considering that videos were seen without any conversational context and that 62 expressions were to be recognized. These results validate our Korean database and, as they also parallel the German validation results, will enable detailed cross-cultural comparisons of the complex space of emotional and conversational expressions.

Remote sensing of traveling ionospheric disturbances resulting from underground nuclear tests

International Nuclear Information System (INIS)

Copenhaver, C.

1985-01-01

Following an underground nuclear test, an acoustic pulse propagates upward through the atmosphere and sets the ionosphere in motion which, in turn, generates gravity waves. The usual ionospheric monitoring approach is to use a phase sounder to observe the acoustic pulse. However, there are other detection techniques that can be employed. These detection techniques include the use of a low-frequency filter so that only long period (approximately 10 minutes) gravity waves can be observed. Another detection technique is to correlate microbarographic measurements on the surface with HF sounder data from the ionosphere to measure Lamb waves. A third detection technique is to correlate seismometer measurements in the ground with their corresponding ionospheric perturbations. The theoretical and experimental aspects of these remote detection techniques are discussed here

Multidimensional Rank Reduction Estimator for Parametric MIMO Channel Models

Directory of Open Access Journals (Sweden)

Marius Pesavento

2004-08-01

Full Text Available A novel algebraic method for the simultaneous estimation of MIMO channel parameters from channel sounder measurements is developed. We consider a parametric multipath propagation model with P discrete paths where each path is characterized by its complex path gain, its directions of arrival and departure, time delay, and Doppler shift. This problem is treated as a special case of the multidimensional harmonic retrieval problem. While the well-known ESPRIT-type algorithms exploit shift-invariance between specific partitions of the signal matrix, the rank reduction estimator (RARE algorithm exploits their internal Vandermonde structure. A multidimensional extension of the RARE algorithm is developed, analyzed, and applied to measurement data recorded with the RUSK vector channel sounder in the 2 GHz band.

Randomized controlled trial of expressive writing for psychological and physical health: the moderating role of emotional expressivity.

Science.gov (United States)

Niles, Andrea N; Haltom, Kate E Byrne; Mulvenna, Catherine M; Lieberman, Matthew D; Stanton, Annette L

2014-01-01

The current study assessed main effects and moderators (including emotional expressiveness, emotional processing, and ambivalence over emotional expression) of the effects of expressive writing in a sample of healthy adults. Young adult participants (N=116) were randomly assigned to write for 20 minutes on four occasions about deepest thoughts and feelings regarding their most stressful/traumatic event in the past five years (expressive writing) or about a control topic (control). Dependent variables were indicators of anxiety, depression, and physical symptoms. No significant effects of writing condition were evident on anxiety, depressive symptoms, or physical symptoms. Emotional expressiveness emerged as a significant moderator of anxiety outcomes, however. Within the expressive writing group, participants high in expressiveness evidenced a significant reduction in anxiety at three-month follow-up, and participants low in expressiveness showed a significant increase in anxiety. Expressiveness did not predict change in anxiety in the control group. These findings on anxiety are consistent with the matching hypothesis, which suggests that matching a person's naturally elected coping approach with an assigned intervention is beneficial. These findings also suggest that expressive writing about a stressful event may be contraindicated for individuals who do not typically express emotions.

The hepatic Raldh1 expression is elevated in Zucker fatty rats and its over-expression introduced the retinal-induced Srebp-1c expression in INS-1 cells.

Directory of Open Access Journals (Sweden)

Yang Li

Full Text Available The roles of vitamin A (VA in the development of metabolic diseases remain unanswered. We have reported that retinoids synergized with insulin to induce the expression of sterol-regulatory element-binding protein 1c gene (Srebp-1c expression in primary rat hepatocytes. Additionally, the hepatic Srebp-1c expression is elevated in Zucker fatty (ZF rats, and reduced in those fed a VA deficient diet. VA is metabolized to retinoic acid (RA for regulating gene expression. We hypothesized that the expression of RA production enzymes contributes to the regulation of the hepatic Srebp-1c expression. Therefore, we analyzed their expression levels in Zucker lean (ZL and ZF rats. The mRNA levels of retinaldehyde dehydrogenase family 1 gene (Raldh1 were found to be higher in the isolated and cultured primary hepatocytes from ZF rats than that from ZL rats. The RALDH1 protein level was elevated in the liver of ZF rats. Retinol and retinal dose- and time-dependently induced the expression of RA responsive Cyp26a1 gene in hepatocytes and hepatoma cells. INS-1 cells were identified as an ideal tool to study the effects of RA production on the regulation of gene expression because only RA, but not retinal, induced Srebp-1c mRNA expression in them. Recombinant adenovirus containing rat Raldh1 cDNA was made and used to infect INS-1 cells. The over-expression of RALDH1 introduced the retinal-mediated induction of Srebp-1c expression in INS-1 cells. We conclude that the expression levels of the enzymes for RA production may contribute to the regulation of RA responsive genes, and determine the responses of the cells to retinoid treatments. The elevated hepatic expression of Raldh1 in ZF rats may cause the excessive RA production from retinol, and in turn, result in higher Srebp-1c expression. This excessive RA production may be one of the factors contributing to the elevated lipogenesis in the liver of ZF rats.

Misrecognition of facial expressions in delinquents

Directory of Open Access Journals (Sweden)

Matsuura Naomi

2009-09-01

Full Text Available Abstract Background Previous reports have suggested impairment in facial expression recognition in delinquents, but controversy remains with respect to how such recognition is impaired. To address this issue, we investigated facial expression recognition in delinquents in detail. Methods We tested 24 male adolescent/young adult delinquents incarcerated in correctional facilities. We compared their performances with those of 24 age- and gender-matched control participants. Using standard photographs of facial expressions illustrating six basic emotions, participants matched each emotional facial expression with an appropriate verbal label. Results Delinquents were less accurate in the recognition of facial expressions that conveyed disgust than were control participants. The delinquents misrecognized the facial expressions of disgust as anger more frequently than did controls. Conclusion These results suggest that one of the underpinnings of delinquency might be impaired recognition of emotional facial expressions, with a specific bias toward interpreting disgusted expressions as hostile angry expressions.

NOAA Climate Data Record (CDR) of Intersatellite Calibrated Clear-Sky HIRS Channel 12 Brightness Temperature, Version 2.6 (Superseded)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This NOAA Climate Data Record (CDR) of Inter-Satellite Calibrated Clear-Sky High Resolution Infrared Radiation Sounder (HIRS) Channel 12 brightness temperatures...

Ionospheric Oblique Incidence Soundings by Satellites

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The oblique incidence sweep-frequency ionospheric sounding technique uses the same principle of operation as the vertical incidence sounder. The primary difference...

Greenland Radar Ice Sheet Thickness Measurements

Data.gov (United States)

National Aeronautics and Space Administration — Two 150-MHz coherent radar depth sounders were developed and flown over the Greenland ice sheet to obtain ice thickness measurements in support of PARCA...

Park7 expression influences myotube size and myosin expression in muscle.

Directory of Open Access Journals (Sweden)

Hui Yu

Full Text Available Callipyge sheep exhibit postnatal muscle hypertrophy due to the up-regulation of DLK1 and/or RTL1. The up-regulation of PARK7 was identified in hypertrophied muscles by microarray analysis and further validated by quantitative PCR. The expression of PARK7 in hypertrophied muscle of callipyge lambs was confirmed to be up-regulated at the protein level. PARK7 was previously identified to positively regulate PI3K/AKT pathway by suppressing the phosphatase activity of PTEN in mouse fibroblasts. The purpose of this study was to investigate the effects of PARK7 in muscle growth and protein accretion in response to IGF1. Primary myoblasts isolated from Park7 (+/+ and Park7 (-/- mice were used to examine the effect of differential expression of Park7. The Park7 (+/+ myotubes had significantly larger diameters and more total sarcomeric myosin expression than Park7 (-/- myotubes. IGF1 treatment increased the mRNA abundance of Myh4, Myh7 and Myh8 between 20-40% in Park7 (+/+ myotubes relative to Park7 (-/-. The level of AKT phosphorylation was increased in Park7 (+/+ myotubes at all levels of IGF1 supplementation. After removal of IGF1, the Park7 (+/+ myotubes maintained higher AKT phosphorylation through 3 hours. PARK7 positively regulates the PI3K/AKT pathway by inhibition of PTEN phosphatase activity in skeletal muscle. The increased PARK7 expression can increase protein synthesis and result in myotube hypertrophy. These results support the hypothesis that elevated expression of PARK7 in callipyge muscle would increase levels of AKT activity to cause hypertrophy in response to the normal IGF1 signaling in rapidly growing lambs. Increasing expression of PARK7 could be a novel mechanism to increase protein accretion and muscle growth in livestock or help improve muscle mass with disease or aging.

Serial Expression Analysis: a web tool for the analysis of serial gene expression data

Science.gov (United States)

Nueda, Maria José; Carbonell, José; Medina, Ignacio; Dopazo, Joaquín; Conesa, Ana

2010-01-01

Serial transcriptomics experiments investigate the dynamics of gene expression changes associated with a quantitative variable such as time or dosage. The statistical analysis of these data implies the study of global and gene-specific expression trends, the identification of significant serial changes, the comparison of expression profiles and the assessment of transcriptional changes in terms of cellular processes. We have created the SEA (Serial Expression Analysis) suite to provide a complete web-based resource for the analysis of serial transcriptomics data. SEA offers five different algorithms based on univariate, multivariate and functional profiling strategies framed within a user-friendly interface and a project-oriented architecture to facilitate the analysis of serial gene expression data sets from different perspectives. SEA is available at sea.bioinfo.cipf.es. PMID:20525784

The Relations of Mothers' Negative Expressivity to Children's Experience and Expression of Negative Emotion

Science.gov (United States)

Valiente, Carlos; Eisenberg, Nancy; Shepard, Stephanie A.; Fabes, Richard A.; Cumberland, Amanda J.; Losoya, Sandra H.; Spinrad, Tracy L.

2004-01-01

Guided by the heuristic model proposed by Eisenberg et al. [Psychol. Inq. 9 (1998) 241], we examined the relations of mothers' reported and observed negative expressivity to children's (N = 159; 74 girls; M age = 7.67 years) experience and expression of emotion. Children's experience and/or expression of emotion in response to a distressing film…

ERG protein expression over time

DEFF Research Database (Denmark)

Berg, Kasper Drimer; Brasso, Klaus; Thomsen, Frederik Birkebæk

2015-01-01

AIMS: We evaluated the consistency in ERG protein expression from diagnostic specimens through rebiopsies to radical prostatectomies in patients with clinically localised prostate cancer to investigate the validity of ERG status in biopsies. METHODS: ERG expression was assessed by immunohistochem......AIMS: We evaluated the consistency in ERG protein expression from diagnostic specimens through rebiopsies to radical prostatectomies in patients with clinically localised prostate cancer to investigate the validity of ERG status in biopsies. METHODS: ERG expression was assessed...

Platelet cyclooxygenase expression in normal dogs.

Science.gov (United States)

Thomason, J; Lunsford, K; Mullins, K; Stokes, J; Pinchuk, L; Wills, R; McLaughlin, R; Langston, C; Pruett, S; Mackin, A

2011-01-01

Human platelets express both cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Variation in COX-2 expression could be a mechanism for variable response to aspirin. The hypotheses were that circulating canine platelets express COX-1 and COX-2, and that aspirin alters COX expression. The objective was to identify changes in platelet COX expression and in platelet function caused by aspirin administration to dogs. Eight female, intact hounds. A single population, repeated measures design was used to evaluate platelet COX-1 and COX-2 expression by flow cytometry before and after aspirin (10 mg/kg Q12h for 10 days). Platelet function was analyzed via PFA-100(®) (collagen/epinephrine), and urine 11-dehydro-thromboxane B(2) (11-dTXB(2)) was measured and normalized to urinary creatinine. Differences in COX expression, PFA-100(®) closure times, and urine 11-dTXB(2 ): creatinine ratio were analyzed before and after aspirin administration. Both COX-1 and COX-2 were expressed in canine platelets. COX-1 mean fluorescent intensity (MFI) increased in all dogs, by 250% (range 63-476%), while COX-2 expression did not change significantly (P = 0.124) after aspirin exposure, with large interindividual variation. PFA-100(®) closure times were prolonged and urine 11-dTXB(2) concentration decreased in all dogs after aspirin administration. Canine platelets express both COX isoforms. After aspirin exposure, COX-1 expression increased despite impairment of platelet function, while COX-2 expression varied markedly among dogs. Variability in platelet COX-2 expression should be explored as a potential mechanism for, or marker of, variable aspirin responsiveness. Copyright © 2011 by the American College of Veterinary Internal Medicine.

Generational Differences of Emotional Expression

Institute of Scientific and Technical Information of China (English)

李学勇

2014-01-01

As a kind of subjective psychological activity, emotion can only be known and perceived by a certain expressive form. Varies as the different main bodies, difference of emotional expression can be reflected not only among individuals but between generations. The old conceals their emotions inside, the young express their emotions boldly, and the middle-aged are rational and deep in their expressions. Facing and understanding such differences is the premise and foundation of the con-struction of a harmonious relationship between different generations.

RSS SSM/I OCEAN PRODUCT GRIDS DAILY FROM DMSP F11 NETCDF V7

Data.gov (United States)

National Aeronautics and Space Administration — This dataset is part of the collection of Special Sensor Microwave/Imager (SSM/I) and Special Sensor Microwave Imager Sounder (SSMIS) data products produced as part...

RSS SSM/I OCEAN PRODUCT GRIDS 3-DAY AVERAGE FROM DMSP F14 NETCDF V7

Data.gov (United States)

National Aeronautics and Space Administration — This dataset is part of the collection of Special Sensor Microwave/Imager (SSM/I) and Special Sensor Microwave Imager Sounder (SSMIS) data products produced as part...

RSS SSMIS OCEAN PRODUCT GRIDS DAILY FROM DMSP F17 NETCDF V7

Data.gov (United States)

National Aeronautics and Space Administration — This dataset is part of the collection of Special Sensor Microwave/Imager (SSM/I) and Special Sensor Microwave Imager Sounder (SSMIS) data products produced as part...

SSMI(S) Hydrological Products

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Special Sensor Microwave/Imager (SSM/I) and Special Sensor Microwave Imager-Sounder (SSMIS) have been making measurements of earth-emitted microwave radiation...

Materiality for Musical Expressions

DEFF Research Database (Denmark)

Lindell, Rikard; Tahiroğlu, Koray; Riis, Morten S.

2016-01-01

Nordic universities. Electronic music instrument makers participated in providing the course. In eleven days the students designed and built interfaces for musical expressions , composed a piece, and performed at the Norberg electronic music festival. The students explored the relationship between......We organised an elven day intense course in materiality for musical expressions to explore underlying principles of New Interfaces for Musical Expression (NIME) in higher education. We grounded the course in different aspects of ma-teriality and gathered interdisciplinary student teams from three...... technology and possible musical expression with a strong connection to culture and place. The emphasis on performance provided closure and motivated teams to move forward in their design and artistic processes. On the basis of the course we discuss an interdisciplinary NIME course syllabus, and we infer...

FTO is expressed in neurones throughout the brain and its expression is unaltered by fasting.

Science.gov (United States)

McTaggart, James S; Lee, Sheena; Iberl, Michaela; Church, Chris; Cox, Roger D; Ashcroft, Frances M

2011-01-01

Single-nucleotide polymorphisms in the first intron of the ubiquitously expressed FTO gene are associated with obesity. Although the physiological functions of FTO remain unclear, food intake is often altered when Fto expression levels are manipulated. Furthermore, deletion of FTO from neurones alone has a similar effect on food intake to deletion of FTO in all tissues. These results indicate that FTO expression in the brain is particularly important. Considerable focus has been placed on the dynamic regulation of Fto mRNA expression in the hypothalamus after short-term (16-48 hour) fasting, but results have been controversial. There are no studies that quantify FTO protein levels across the brain, and assess its alteration following short-term fasting. Using immunohistochemistry, we found that FTO protein is widely expressed in mouse brain, and present in the majority of neurones. Using quantitative Western blotting and RT-qPCR we show that FTO protein and mRNA levels in the hypothalamus, cerebellum and rostral brain are relatively uniform, and levels in the brain are higher than in skeletal muscles of the lower limbs. Fasting for 18 hours does not alter the expression pattern, or levels, of FTO protein and mRNA. We further show that the majority of POMC neurones, which are critically involved in food intake regulation, also express FTO, but that the percentage of FTO-positive POMC neurones is not altered by fasting. In summary, we find no evidence that Fto/FTO expression is regulated by short-term (18-hour) fasting. Thus, it is unlikely that the hunger and increased post-fasting food intake caused by such food deprivation is driven by alterations in Fto/FTO expression. The widespread expression of FTO in neurones also suggests that physiological studies of this protein should not be limited to the hypothalamus.

FTO is expressed in neurones throughout the brain and its expression is unaltered by fasting.

Directory of Open Access Journals (Sweden)

James S McTaggart

Full Text Available Single-nucleotide polymorphisms in the first intron of the ubiquitously expressed FTO gene are associated with obesity. Although the physiological functions of FTO remain unclear, food intake is often altered when Fto expression levels are manipulated. Furthermore, deletion of FTO from neurones alone has a similar effect on food intake to deletion of FTO in all tissues. These results indicate that FTO expression in the brain is particularly important. Considerable focus has been placed on the dynamic regulation of Fto mRNA expression in the hypothalamus after short-term (16-48 hour fasting, but results have been controversial. There are no studies that quantify FTO protein levels across the brain, and assess its alteration following short-term fasting. Using immunohistochemistry, we found that FTO protein is widely expressed in mouse brain, and present in the majority of neurones. Using quantitative Western blotting and RT-qPCR we show that FTO protein and mRNA levels in the hypothalamus, cerebellum and rostral brain are relatively uniform, and levels in the brain are higher than in skeletal muscles of the lower limbs. Fasting for 18 hours does not alter the expression pattern, or levels, of FTO protein and mRNA. We further show that the majority of POMC neurones, which are critically involved in food intake regulation, also express FTO, but that the percentage of FTO-positive POMC neurones is not altered by fasting. In summary, we find no evidence that Fto/FTO expression is regulated by short-term (18-hour fasting. Thus, it is unlikely that the hunger and increased post-fasting food intake caused by such food deprivation is driven by alterations in Fto/FTO expression. The widespread expression of FTO in neurones also suggests that physiological studies of this protein should not be limited to the hypothalamus.

Effectively identifying regulatory hotspots while capturing expression heterogeneity in gene expression studies

Science.gov (United States)

2014-01-01

Expression quantitative trait loci (eQTL) mapping is a tool that can systematically identify genetic variation affecting gene expression. eQTL mapping studies have shown that certain genomic locations, referred to as regulatory hotspots, may affect the expression levels of many genes. Recently, studies have shown that various confounding factors may induce spurious regulatory hotspots. Here, we introduce a novel statistical method that effectively eliminates spurious hotspots while retaining genuine hotspots. Applied to simulated and real datasets, we validate that our method achieves greater sensitivity while retaining low false discovery rates compared to previous methods. PMID:24708878

NCAM (CD56) expression in keratin-producing odontogenic cysts: aberrant expression in KCOT.

Science.gov (United States)

Vera-Sirera, Beatriz; Forner-Navarro, Leopoldo; Vera-Sempere, Francisco

2015-02-12

To investigate immunohistochemically the expression of neural cell adhesion molecule (NCAM), which has been identified as a signaling receptor with frequent reactivity in ameloblastomas (AB), in a series of keratin-producing odontogenic cysts (KPOCs). Immunohistochemical expression of NCAM, using a monoclonal antibody, was determined in a series of 58 KPOCs comprising 12 orthokeratinized odontogenic cysts (OOCs) and 46 keratocystic odontogenic tumors (KCOTs), corresponding to 40 non-syndromic KCOT (NS-KCOTs) and 6 syndromic KCOT (S-KCOTs), associated with nevic basocellular syndrome (NBCS). NCAM expression was negative in all OOCs, but 36.45% of KCOTs exhibited focal and heterogeneous expression at the basal cell level, as well as in basal budding areas and the basal cells of daughter cysts. The latter two locations were especially applicable to S-KCOTs, with focal NCAM reactivity occurring in 66.66% of cases. Aberrant NCAM expression, in KCOTs but especially in S-KCOTs, together with its immunomorphological location, suggests that this adhesion molecule and signaling receptor plays a role in the pathogenesis of KCOTs, with a probable impact on lesional recurrence.

Expressiveness in musical performance: Pedagogic aspect

Directory of Open Access Journals (Sweden)

Jović Natalija R.

2016-01-01

Full Text Available The subject of our research relates to pedagogic aspects of expressive vocal-instrumental musical performance. We intended to examine: (1 how undergraduate students see/conceptualize and evaluate expressiveness in musical performance; (2 whether and how they were trained in the skill of expressive musical performance during their musical training; (3 whether and in which way they rehearse the expressive component of musical performance and interpretation and (4 whether there are any differences regarding gender, age, instrument, department, year of study and years of instrument playing in relation to the group of dependant variables related to expressiveness, tuition and practice. The sample for the research included 82 students of instrumental and theory departments at the Faculty of Music in Belgrade. Psychological and pedagogical aspects of musical expressiveness during vocal-instrumental performance were analyzed. The results show that students highly evaluate expressiveness but its place is secondary compared to mastering technical and tonal requirements. Statistically significant differences were shown regarding gender, age and departments. It can be concluded that there is a potential for the development and enhancement of expressiveness of students if we abandon the traditional view that expressiveness is linked exclusively to talent. The findings indicate that pedagogical work should be directed towards finding purposeful strategies for training individual expressiveness.

Algorithm integration using ADL (Algorithm Development Library) for improving CrIMSS EDR science product quality

Science.gov (United States)

Das, B.; Wilson, M.; Divakarla, M. G.; Chen, W.; Barnet, C.; Wolf, W.

2013-05-01

Algorithm Development Library (ADL) is a framework that mimics the operational system IDPS (Interface Data Processing Segment) that is currently being used to process data from instruments aboard Suomi National Polar-orbiting Partnership (S-NPP) satellite. The satellite was launched successfully in October 2011. The Cross-track Infrared and Microwave Sounder Suite (CrIMSS) consists of the Advanced Technology Microwave Sounder (ATMS) and Cross-track Infrared Sounder (CrIS) instruments that are on-board of S-NPP. These instruments will also be on-board of JPSS (Joint Polar Satellite System) that will be launched in early 2017. The primary products of the CrIMSS Environmental Data Record (EDR) include global atmospheric vertical temperature, moisture, and pressure profiles (AVTP, AVMP and AVPP) and Ozone IP (Intermediate Product from CrIS radiances). Several algorithm updates have recently been proposed by CrIMSS scientists that include fixes to the handling of forward modeling errors, a more conservative identification of clear scenes, indexing corrections for daytime products, and relaxed constraints between surface temperature and air temperature for daytime land scenes. We have integrated these improvements into the ADL framework. This work compares the results from ADL emulation of future IDPS system incorporating all the suggested algorithm updates with the current official processing results by qualitative and quantitative evaluations. The results prove these algorithm updates improve science product quality.

Expression of MIF and CD74 in leukemic cell lines: correlation to DR expression destiny.

Science.gov (United States)

Georgouli, Mirella; Papadimitriou, Lina; Glymenaki, Maria; Patsaki, Valia; Athanassakis, Irene

2016-06-01

Invariant chain (Ii) or CD74 is a non-polymorphic glycoprotein, which apart from its role as a chaperone dedicated to MHCII molecules, is known to be a high-affinity receptor for macrophage migration inhibitory factor (MIF). The present study aimed to define the roles of CD74 and MIF in the immune surveillance escape process. Towards this direction, the cell lines HL-60, Raji, K562 and primary pre-B leukemic cells were examined for expression and secretion of MIF. Flow cytometry analysis detected high levels of MIF and intracellular/membrane CD74 expression in all leukemic cells tested, while MIF secretion was shown to be inversely proportional to intracellular HLA-DR (DR) expression. In the MHCII-negative cells, IFN-γ increased MIF expression and induced its secretion in HL-60 and K562 cells, respectively. In K562 cells, CD74 (Iip33Iip35) was shown to co-precipitate with HLA-DOβ (DOβ), inhibiting thus MIF or DR binding. Induced expression of DOα in K562 (DOα-DOβ+) cells in different transfection combinations decreased MIF expression and secretion, while increasing surface DR expression. Thus, MIF could indeed be part of the antigen presentation process.

Exercise-induced liver chemokine CXCL-1 expression is linked to muscle-derived interleukin-6 expression

DEFF Research Database (Denmark)

Pedersen, Line; Pilegaard, Henriette; Hansen, Jakob

2011-01-01

interleukin-6 (IL-6) and muscle IL-6 mRNA. In contrast, exercise-induced regulation of liver CXCL-1 mRNA expression was completely blunted in IL-6 knockout mice. Based on these findings, we examined the possible existence of a muscle-to-liver axis by overexpressing IL-6 in muscles. This resulted in increases...... in serum CXCL-1 (5-fold) and liver CXCL-1 mRNA expression (24-fold) compared with control. Because IL-6 expression and release are known to be augmented during exercise in glycogen-depleted animals, CXCL-1 and IL-6 expression were examined after exercise in overnight-fasted mice.We found that fasting...... significantly augmented serum CXCL-1, and CXCL-1 expression in liver and muscle. Taken together, these data indicate that liver is the main source of serum CXCL-1 during exercise in mice, and that the CXCL-1 expression in the liver is regulated by muscle-derived IL-6....

An enhanced Planetary Radar Operating Centre (PROC)

Science.gov (United States)

Catallo, C.

2010-12-01

Planetary exploration by means of radar systems, mainly using GPRs is an important role of Italy and numerous scientific international space programs are carried out jointly with ESA and NASA by Italian Space Agency, the scientific community and the industry. Three experiments under Italian leadership ( designed and manufactured by the Italian industry) provided by ASI within a NASA/ESA/ASI joint venture framework are successfully operating: MARSIS on-board MEX, SHARAD on-board MRO and CASSINI Radar on-board Cassini spacecraft: the missions have been further extended . Three dedicated operational centers, namely SHOC, (Sharad Operating Centre), MOC (Marsis Operating Center) and CASSINI PAD are operating from the missions beginning to support all the scientific communities, institutional customers and experiment teams operation Each center is dedicated to a single instrument management and control, data processing and distribution and even if they had been conceived to operate autonomously and independently one from each other, synergies and overlaps have been envisaged leading to the suggestion of a unified center, the Planetary Radar Processing Center (PROC). In order to harmonize operations either from logistics point of view and from HW/SW capabilities point of view PROC is designed and developed for offering improved functionalities to increase capabilities, mainly in terms of data exchange, comparison, interpretation and exploitation. PROC is, therefore, conceived as the Italian support facility to the scientific community for on-going and future Italian planetary exploration programs, such as Europa-Jupiter System Mission (EJSM) The paper describes how the new PROC is designed and developed, to allow SHOC, MOC and CASSINI PAD to operate as before, and to offer improved functionalities to increase capabilities, mainly in terms of data exchange, comparison, interpretation and exploitation aiding scientists to increase their knowledge in the field of surface

Oracle Application Express 4 Recipes

CERN Document Server

Zehoo, Edmund

2011-01-01

Oracle Application Express 4 Recipes provides an example-based approach to learning Application Express - the ground-breaking, rapid application development platform included with every Oracle Database license. The recipes format is ideal for the quick-study who just wants a good example or two to kick start their thinking and get pointed in the right direction. The recipes cover the gamut of Application Express development. Author and Application Express expert Edmund Zehoo shows how to create data entry screens, visualize data in the form of reports and charts, implement validation and back-

MLS/Aura L2 Chlorine Monoxide (ClO) Mixing Ratio V002

Data.gov (United States)

National Aeronautics and Space Administration — ML2CLO is the EOS Aura Microwave Limb Sounder (MLS) standard product for chlorine monoxide derived from radiances measured primarily by the 640 GHz radiometer. The...

Aqua AIRS L2 Standard Physical Retrieval (AIRS+AMSU+HSB) V006

Data.gov (United States)

National Aeronautics and Space Administration — This product is similar to AIRX2RET. However, it contains science retrievals that use the Humidity Sounder for Brazil (HSB). Because the HSB instrument lived only...

AIRS/Aqua Level 2 Standard physical retrieval (AIRS+AMSU+HSB) V005

Data.gov (United States)

National Aeronautics and Space Administration — This product is similar to AIRX2RET. However, it contains science retrievals that use the Humidity Sounder for Brazil (HSB). Because the HSB instrument lived only...

Aqua AIRS L2 Cloud-Cleared Infrared Radiances (AIRS+AMSU+HSB) V006

Data.gov (United States)

National Aeronautics and Space Administration — This product is similar to AIRI2CCF. However, it contains science retrievals that use the Humidity Sounder for Brazil (HSB). Because the HSB instrument lived only...

MLS/Aura L2 Hydrogen Chloride (HCl) Mixing Ratio V003

Data.gov (United States)

National Aeronautics and Space Administration — ML2HCL is the EOS Aura Microwave Limb Sounder (MLS) standard product for hydrogen chloride derived from radiances measured primarily by the 640 GHz radiometer. The...

MLS/Aura L2 Hydrogen Cyanide (HCN) Mixing Ratio V003

Data.gov (United States)

National Aeronautics and Space Administration — ML2HCN is the EOS Aura Microwave Limb Sounder (MLS) standard product for hydrogen cyanide derived from radiances measured primarily by the 190 GHz radiometer. The...

MLS/Aura L2 Hydrogen Cyanide (HCN) Mixing Ratio V002

Data.gov (United States)

National Aeronautics and Space Administration — ML2HCN is the EOS Aura Microwave Limb Sounder (MLS) standard product for hydrogen cyanide derived from radiances measured primarily by the 190 GHz radiometer. The...

MLS/Aura Level 2 Hydrogen Chloride (HCl) Mixing Ratio V004

Data.gov (United States)

National Aeronautics and Space Administration — ML2HCL is the EOS Aura Microwave Limb Sounder (MLS) standard product for hydrogen chloride derived from radiances measured primarily by the 640 GHz radiometer. The...

Aqua AIRS L2 Support Retrieval (AIRS+AMSU+HSB) V006

Data.gov (United States)

National Aeronautics and Space Administration — This product is similar to AIRX2SUP. However, it contains science retrievals that use the Humidity Sounder for Brazil (HSB). Because the HSB instrument lived only...

AIRS/Aqua Level 2 Support retrieval (AIRS+AMSU+HSB) V005

Data.gov (United States)

National Aeronautics and Space Administration — This product is similar to AIRX2SUP. However, it contains science retrievals that use the Humidity Sounder for Brazil (HSB). Because the HSB instrument lived only...

AIRS/Aqua L2 Near Real Time (NRT) Cloud-Cleared Infrared Radiances (AIRS-only) V006 (AIRS2CCF_NRT) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — The Atmospheric Infrared Sounder (AIRS) Level 2 Near Real Time (NRT) Cloud-Cleared Infrared Radiances (AIRS-only) product (AIRS2CCF_NRT_006) differs from the routine...

NOAA Fundamental Climate Data Record (CDR) of AMSU-B and MHS Brightness Temperature, Version 1

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA Climate Data Record (CDR) of Advanced Microwave Sounding Unit-B (AMSU-B) and Microwave Humidity Sounder (MHS) brightness temperature (Tb) in "window...

Sediment Types Determination Using Acoustic Techniques in the Northeastern Gulf of Mexico

National Research Council Canada - National Science Library

Kim, Gil

2004-01-01

... those (acoustic impedance and grain size) in the northeastern Gulf of Mexico. The acoustic data were acquired using a 11 kHz normal incident echo sounder over approximately 2000 km of track line...

MLS/Aura L2 Hypochlorous Acid (HOCL) Mixing Ratio V003

Data.gov (United States)

National Aeronautics and Space Administration — ML2HOCL is the EOS Aura Microwave Limb Sounder (MLS) standard product for hypochlorous acid derived from radiances measured primarily by the 640 GHz radiometer. The...

MLS/Aura Level 2 Hypochlorous Acid (HOCl) Mixing Ratio V004

Data.gov (United States)

National Aeronautics and Space Administration — ML2HOCL is the EOS Aura Microwave Limb Sounder (MLS) standard product for hypochlorous acid derived from radiances measured primarily by the 640 GHz radiometer. The...

MLS/Aura L2 Hypochlorous Acid (HOCL) Mixing Ratio V002

Data.gov (United States)

National Aeronautics and Space Administration — ML2HOCL is the EOS Aura Microwave Limb Sounder (MLS) standard product for hypochlorous acid derived from radiances measured primarily by the 640 GHz radiometer. The...

MLS/Aura Level 2 Hypochlorous Acid (HOCl) Mixing Ratio V003 (ML2HOCL) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — ML2HOCL is the EOS Aura Microwave Limb Sounder (MLS) standard product for hypochlorous acid derived from radiances measured primarily by the 640 GHz radiometer. The...

AIRS/Aqua Level 3 Daily standard physical retrieval (AIRS+AMSU+HSB) V005

Data.gov (United States)

National Aeronautics and Space Administration — This product is similar to AIRX3STD. However, it contains science retrievals that use the Humidity Sounder for Brazil (HSB). Because the HSB instrument lived only...

AIRS/Aqua Level 3 Monthly standard physical retrieval (AIRS+AMSU+HSB) V005

Data.gov (United States)

National Aeronautics and Space Administration — This product is similar to AIRX3STM. However, it contains science retrievals that use the Humidity Sounder for Brazil (HSB). Because the HSB instrument lived only...

Expression, Delivery and Function of Insecticidal Proteins Expressed by Recombinant Baculoviruses

Science.gov (United States)

Kroemer, Jeremy A.; Bonning, Bryony C.; Harrison, Robert L.

2015-01-01

Since the development of methods for inserting and expressing genes in baculoviruses, a line of research has focused on developing recombinant baculoviruses that express insecticidal peptides and proteins. These recombinant viruses have been engineered with the goal of improving their pesticidal potential by shortening the time required for infection to kill or incapacitate insect pests and reducing the quantity of crop damage as a consequence. A wide variety of neurotoxic peptides, proteins that regulate insect physiology, degradative enzymes, and other potentially insecticidal proteins have been evaluated for their capacity to reduce the survival time of baculovirus-infected lepidopteran host larvae. Researchers have investigated the factors involved in the efficient expression and delivery of baculovirus-encoded insecticidal peptides and proteins, with much effort dedicated to identifying ideal promoters for driving transcription and signal peptides that mediate secretion of the expressed target protein. Other factors, particularly translational efficiency of transcripts derived from recombinant insecticidal genes and post-translational folding and processing of insecticidal proteins, remain relatively unexplored. The discovery of RNA interference as a gene-specific regulation mechanism offers a new approach for improvement of baculovirus biopesticidal efficacy through genetic modification. PMID:25609310

Homeobox gene expression in Brachiopoda

DEFF Research Database (Denmark)

Altenburger, Andreas; Martinez, Pedro; Wanninger, Andreas

2011-01-01

(ectoderm) specification with co-opted functions in notochord formation in chordates and left/right determination in ambulacrarians and vertebrates. The caudal ortholog, TtrCdx, is first expressed in the ectoderm of the gastrulating embryo in the posterior region of the blastopore. Its expression stays......The molecular control that underlies brachiopod ontogeny is largely unknown. In order to contribute to this issue we analyzed the expression pattern of two homeobox containing genes, Not and Cdx, during development of the rhynchonelliform (i.e., articulate) brachiopod Terebratalia transversa...... completion of larval development, which is marked by a three-lobed body with larval setae. Expression starts at gastrulation in two areas lateral to the blastopore and subsequently extends over the animal pole of the gastrula. With elongation of the gastrula, expression at the animal pole narrows to a small...

A polynomial time biclustering algorithm for finding approximate expression patterns in gene expression time series

Directory of Open Access Journals (Sweden)

Madeira Sara C

2009-06-01

Full Text Available Abstract Background The ability to monitor the change in expression patterns over time, and to observe the emergence of coherent temporal responses using gene expression time series, obtained from microarray experiments, is critical to advance our understanding of complex biological processes. In this context, biclustering algorithms have been recognized as an important tool for the discovery of local expression patterns, which are crucial to unravel potential regulatory mechanisms. Although most formulations of the biclustering problem are NP-hard, when working with time series expression data the interesting biclusters can be restricted to those with contiguous columns. This restriction leads to a tractable problem and enables the design of efficient biclustering algorithms able to identify all maximal contiguous column coherent biclusters. Methods In this work, we propose e-CCC-Biclustering, a biclustering algorithm that finds and reports all maximal contiguous column coherent biclusters with approximate expression patterns in time polynomial in the size of the time series gene expression matrix. This polynomial time complexity is achieved by manipulating a discretized version of the original matrix using efficient string processing techniques. We also propose extensions to deal with missing values, discover anticorrelated and scaled expression patterns, and different ways to compute the errors allowed in the expression patterns. We propose a scoring criterion combining the statistical significance of expression patterns with a similarity measure between overlapping biclusters. Results We present results in real data showing the effectiveness of e-CCC-Biclustering and its relevance in the discovery of regulatory modules describing the transcriptomic expression patterns occurring in Saccharomyces cerevisiae in response to heat stress. In particular, the results show the advantage of considering approximate patterns when compared to state of

Characterizing sampling and quality screening biases in infrared and microwave limb sounding

Science.gov (United States)

Millán, Luis F.; Livesey, Nathaniel J.; Santee, Michelle L.; von Clarmann, Thomas

2018-03-01

This study investigates orbital sampling biases and evaluates the additional impact caused by data quality screening for the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) and the Aura Microwave Limb Sounder (MLS). MIPAS acts as a proxy for typical infrared limb emission sounders, while MLS acts as a proxy for microwave limb sounders. These biases were calculated for temperature and several trace gases by interpolating model fields to real sampling patterns and, additionally, screening those locations as directed by their corresponding quality criteria. Both instruments have dense uniform sampling patterns typical of limb emission sounders, producing almost identical sampling biases. However, there is a substantial difference between the number of locations discarded. MIPAS, as a mid-infrared instrument, is very sensitive to clouds, and measurements affected by them are thus rejected from the analysis. For example, in the tropics, the MIPAS yield is strongly affected by clouds, while MLS is mostly unaffected. The results show that upper-tropospheric sampling biases in zonally averaged data, for both instruments, can be up to 10 to 30 %, depending on the species, and up to 3 K for temperature. For MIPAS, the sampling reduction due to quality screening worsens the biases, leading to values as large as 30 to 100 % for the trace gases and expanding the 3 K bias region for temperature. This type of sampling bias is largely induced by the geophysical origins of the screening (e.g. clouds). Further, analysis of long-term time series reveals that these additional quality screening biases may affect the ability to accurately detect upper-tropospheric long-term changes using such data. In contrast, MLS data quality screening removes sufficiently few points that no additional bias is introduced, although its penetration is limited to the upper troposphere, while MIPAS may cover well into the mid-troposphere in cloud-free scenarios. We emphasize that the

Analysis of Facial Expression by Taste Stimulation

Science.gov (United States)

Tobitani, Kensuke; Kato, Kunihito; Yamamoto, Kazuhiko

In this study, we focused on the basic taste stimulation for the analysis of real facial expressions. We considered that the expressions caused by taste stimulation were unaffected by individuality or emotion, that is, such expressions were involuntary. We analyzed the movement of facial muscles by taste stimulation and compared real expressions with artificial expressions. From the result, we identified an obvious difference between real and artificial expressions. Thus, our method would be a new approach for facial expression recognition.

Osteopontin expression in salivary gland carcinomas

DEFF Research Database (Denmark)

Bjørndal, Kristine; Larsen, Stine R; Godballe, Christian

2011-01-01

J Oral Pathol Med (2010) Background:  In several cancer types, osteopontin (OPN) expression has been correlated with tumor progression and prognosis. Two earlier studies have examined OPN expression in salivary gland carcinomas with contradictory results. Methods:  One hundred and seventy......:  Osteopontin was expressed in all salivary gland carcinomas. Adenoid cystic carcinomas had the highest mean sum score (7.3) and a significantly higher proportion of carcinomas with high OPN sum score than both mucoepidermoid carcinoma and acinic cell carcinoma. Correlation of OPN expression with known...... prognostic factors in salivary gland carcinomas was insignificant. Conclusions:  Salivary gland carcinomas express OPN. The expression does not correlate with known prognostic factors....

MLS/Aura L2 Geopotential Height V003

Data.gov (United States)

National Aeronautics and Space Administration — ML2GPH is the EOS Aura Microwave Limb Sounder (MLS) standard product for geopotential height derived from radiances measured by the 118 and 240 GHz radiometers. The...

MLS/Aura L2 Geopotential Height V002

Data.gov (United States)

National Aeronautics and Space Administration — ML2GPH is the EOS Aura Microwave Limb Sounder (MLS) standard product for geopotential height derived from radiances measured by the 118 and 240 GHz radiometers. The...

NOAA Climate Data Record (CDR) of Monthly Outgoing Longwave Radiation (OLR), Version 2.2-1

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This Climate Data Record (CDR) of monthly mean High Resolution Infrared Radiation Sounder (HIRS) Outgoing Longwave Radiation (OLR) flux at the top of the atmosphere...

Regular expression containment

DEFF Research Database (Denmark)

Henglein, Fritz; Nielsen, Lasse

2011-01-01

We present a new sound and complete axiomatization of regular expression containment. It consists of the conventional axiomatiza- tion of concatenation, alternation, empty set and (the singleton set containing) the empty string as an idempotent semiring, the fixed- point rule E* = 1 + E × E......* for Kleene-star, and a general coin- duction rule as the only additional rule. Our axiomatization gives rise to a natural computational inter- pretation of regular expressions as simple types that represent parse trees, and of containment proofs as coercions. This gives the axiom- atization a Curry......-Howard-style constructive interpretation: Con- tainment proofs do not only certify a language-theoretic contain- ment, but, under our computational interpretation, constructively transform a membership proof of a string in one regular expres- sion into a membership proof of the same string in another regular expression. We...

Polycistronic gene expression in Aspergillus niger.

Science.gov (United States)

Schuetze, Tabea; Meyer, Vera

2017-09-25

Genome mining approaches predict dozens of biosynthetic gene clusters in each of the filamentous fungal genomes sequenced so far. However, the majority of these gene clusters still remain cryptic because they are not expressed in their natural host. Simultaneous expression of all genes belonging to a biosynthetic pathway in a heterologous host is one approach to activate biosynthetic gene clusters and to screen the metabolites produced for bioactivities. Polycistronic expression of all pathway genes under control of a single and tunable promoter would be the method of choice, as this does not only simplify cloning procedures, but also offers control on timing and strength of expression. However, polycistronic gene expression is a feature not commonly found in eukaryotic host systems, such as Aspergillus niger. In this study, we tested the suitability of the viral P2A peptide for co-expression of three genes in A. niger. Two genes descend from Fusarium oxysporum and are essential to produce the secondary metabolite enniatin (esyn1, ekivR). The third gene (luc) encodes the reporter luciferase which was included to study position effects. Expression of the polycistronic gene cassette was put under control of the Tet-On system to ensure tunable gene expression in A. niger. In total, three polycistronic expression cassettes which differed in the position of luc were constructed and targeted to the pyrG locus in A. niger. This allowed direct comparison of the luciferase activity based on the position of the luciferase gene. Doxycycline-mediated induction of the Tet-On expression cassettes resulted in the production of one long polycistronic mRNA as proven by Northern analyses, and ensured comparable production of enniatin in all three strains. Notably, gene position within the polycistronic expression cassette matters, as, luciferase activity was lowest at position one and had a comparable activity at positions two and three. The P2A peptide can be used to express at

dictyExpress: a Dictyostelium discoideum gene expression database with an explorative data analysis web-based interface

Science.gov (United States)

Rot, Gregor; Parikh, Anup; Curk, Tomaz; Kuspa, Adam; Shaulsky, Gad; Zupan, Blaz

2009-01-01

Background Bioinformatics often leverages on recent advancements in computer science to support biologists in their scientific discovery process. Such efforts include the development of easy-to-use web interfaces to biomedical databases. Recent advancements in interactive web technologies require us to rethink the standard submit-and-wait paradigm, and craft bioinformatics web applications that share analytical and interactive power with their desktop relatives, while retaining simplicity and availability. Results We have developed dictyExpress, a web application that features a graphical, highly interactive explorative interface to our database that consists of more than 1000 Dictyostelium discoideum gene expression experiments. In dictyExpress, the user can select experiments and genes, perform gene clustering, view gene expression profiles across time, view gene co-expression networks, perform analyses of Gene Ontology term enrichment, and simultaneously display expression profiles for a selected gene in various experiments. Most importantly, these tasks are achieved through web applications whose components are seamlessly interlinked and immediately respond to events triggered by the user, thus providing a powerful explorative data analysis environment. Conclusion dictyExpress is a precursor for a new generation of web-based bioinformatics applications with simple but powerful interactive interfaces that resemble that of the modern desktop. While dictyExpress serves mainly the Dictyostelium research community, it is relatively easy to adapt it to other datasets. We propose that the design ideas behind dictyExpress will influence the development of similar applications for other model organisms. PMID:19706156

Differential Gene Expression and Aging

Directory of Open Access Journals (Sweden)

Laurent Seroude

2002-01-01

Full Text Available It has been established that an intricate program of gene expression controls progression through the different stages in development. The equally complex biological phenomenon known as aging is genetically determined and environmentally modulated. This review focuses on the genetic component of aging, with a special emphasis on differential gene expression. At least two genetic pathways regulating organism longevity act by modifying gene expression. Many genes are also subjected to age-dependent transcriptional regulation. Some age-related gene expression changes are prevented by caloric restriction, the most robust intervention that slows down the aging process. Manipulating the expression of some age-regulated genes can extend an organism's life span. Remarkably, the activity of many transcription regulatory elements is linked to physiological age as opposed to chronological age, indicating that orderly and tightly controlled regulatory pathways are active during aging.

Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177

DEFF Research Database (Denmark)

Hu, Nan; Mora-Jensen, Helena; Theilgaard-Mønch, Kim

2014-01-01

OBJECTIVE: Differential gene expression in CD177+ and CD177- neutrophils was investigated, in order to detect possible differences in neutrophil function which could be related to the pathogenesis of ANCA-associated Vasculitides (AAV). METHODS: Neutrophils were isolated from healthy controls (HC......) with high, negative or bimodal CD177 expression, and sorted into CD177+ and CD177- subpopulations. Total RNA was screened for expression of 24,000 probes with Illumina Ref-8 Beadchips. Genes showing differential expression between CD177+ and CD177- subsets in microarray analysis were re-assessed using...... quantitative-PCR. CD177 expression on neutrophil precursors in bone marrow was analyzed using quantitative PCR and flowcytometry. RESULTS: The proportion of CD177+ cells increased during neutrophil maturation in bone marrow. Fold change analysis of gene expression profile of sorted CD177+ and CD177...

Meteorological research studies at Jervis Bay, Australia

International Nuclear Information System (INIS)

Clark, G.H.; Bendun, E.O.K.

1974-07-01

A climatological study of the winds and temperature from the Jervis Bay region which commenced in October 1970 has shown the presence of a coastal sea breeze and secondary bay breeze circulation system. In an attempt to define the influence of the Murray's Beach site on the local atmospheric dispersion, special smoke plume photography studies were conducted in the lower atmosphere. In June 1972 a meteorological acoustic sounding research programme was initiated at the Jervis Bay settlement. The aims of the research are to calibrate the sounder in terms of surface wind, turbulence and temperature measurements pertinent to a description of the lower atmospheric dispersion potential. Preliminary results on six months' data have shown encouraging correlations between the acoustic sounder patterns and particularly the wind direction turbulence traces. (author)

ISAMS and MLS for NASA's Upper Atmosphere Research Satellite

Science.gov (United States)

Llewellyn-Jones, D.; Dickinson, P. H. G.

1990-04-01

The primary goal of NASA's Upper Atmosphere Research Satellite (UARS), planned to be launched in 1991, is to compile data about the structure and behavior of the stratospheric ozone layer, and especially about the threat of the chlorine-based pollutants to its stablility. Two of the payload instruments, manufactured in the UK, are described: the Improved Stratospheric and Mesospheric Sounder (ISAMS), a radiometer designed to measure thermal emission from selected atmospheric constituents at the earth's limb, then making it possible to obtain nearly global coverage of the vertical distribution of temperature and composition from 80 deg S to 80 deg N latitude; and the Microwave Limb Sounder (MLS), a limb sounding radiometer, measuring atmospheric thermal emission from selected molecular spectral lines at mm wavelength, in the frequency regions of 63, 183, and 205 GHz.

Recognising and Interpreting Named Temporal Expressions

DEFF Research Database (Denmark)

Brucato, Matteo; Derczynski, Leon; Llorens, Hectjor

2013-01-01

This paper introduces a new class of temporal expression – named temporal expressions – and methods for recognising and interpreting its members. The commonest temporal expressions typically contain date and time words, like April or hours. Research into recognising and interpreting these typical...... expressions is mature in many languages. However, there is a class of expressions that are less typical, very varied, and difficult to automatically interpret. These indicate dates and times, but are harder to detect because they often do not contain time words and are not used frequently enough to appear...

Expressing Model Constraints Visually with VMQL

DEFF Research Database (Denmark)

Störrle, Harald

2011-01-01

) for specifying constraints on UML models. We examine VMQL's usability by controlled experiments and its expressiveness by a representative sample. We conclude that VMQL is less expressive than OCL, although expressive enough for most of the constraints in the sample. In terms of usability, however, VMQL......OCL is the de facto standard language for expressing constraints and queries on UML models. However, OCL expressions are very difficult to create, understand, and maintain, even with the sophisticated tool support now available. In this paper, we propose to use the Visual Model Query Language (VMQL...

Neuropilin-2 expression in breast cancer: correlation with lymph node metastasis, poor prognosis, and regulation of CXCR4 expression

International Nuclear Information System (INIS)

Yasuoka, Hironao; Kodama, Rieko; Tsujimoto, Masahiko; Yoshidome, Katsuhide; Akamatsu, Hiroki; Nakahara, Masaaki; Inagaki, Michiya; Sanke, Tokio; Nakamura, Yasushi

2009-01-01

Neuropilin-2 (Nrp2) is a receptor for vascular endothelial growth factor-C (VEGF-C), which is a well-known lymphangiogenic factor and plays an important role in lymph node metastasis of various human cancers, including breast cancer. Recently, Nrp2 was shown to play a role in cancer by promoting tumor cell metastasis. CXC chemokine receptor 4 (CXCR4) also promotes tumor metastasis. In the previous studies, we demonstrated that VEGF-C and cytoplasmic CXCR4 expressions were correlated with poorer patient prognosis (BMC Cancer 2008,8:340; Breast Cancer Res Treat 2005, 91:125–132). The relationship between Nrp2 expression and lymph node metastasis, VEGF-C expression, CXCR4 expression, and other established clinicopathological variables (these data were cited in our previous papers), including prognosis, was analyzed in human breast cancer. Effects of neutralizing anti-Nrp2 antibody on CXCR4 expression and chemotaxis were assessed in MDA-MB-231 breast cancer cells. Nrp2 expression was observed in 53.1% (60 of 113) of the invasive breast carcinomas. Nrp2 expression was significantly correlated with lymph node metastasis, VEGF-C expression, and cytoplasmic CXCR4 expression. Survival curves determined by the Kaplan-Meier method showed that Nrp2 expression was associated with reduced overall survival. In multivariate analysis, Nrp2 expression emerged as a significant independent predictor for overall survival. Neutralizing anti-Nrp2 antibody blocks cytoplasmic CXCR4 expression and CXCR4-induced migration in MDA-MB-231 cells. Nrp2 expression was correlated with lymph node metastasis, VEGF-C expression, and cytoplasmic CXCR4 expression. Nrp2 expression may serve as a significant prognostic factor for long-term survival in breast cancer. Our data also showed a role for Nrp2 in regulating cytoplasmic CXCR4 expression in vitro

Recognizing Facial Expressions Automatically from Video

Science.gov (United States)

Shan, Caifeng; Braspenning, Ralph

Facial expressions, resulting from movements of the facial muscles, are the face changes in response to a person's internal emotional states, intentions, or social communications. There is a considerable history associated with the study on facial expressions. Darwin [22] was the first to describe in details the specific facial expressions associated with emotions in animals and humans, who argued that all mammals show emotions reliably in their faces. Since that, facial expression analysis has been a area of great research interest for behavioral scientists [27]. Psychological studies [48, 3] suggest that facial expressions, as the main mode for nonverbal communication, play a vital role in human face-to-face communication. For illustration, we show some examples of facial expressions in Fig. 1.

Scaling of gene expression data allowing the comparison of different gene expression platforms

NARCIS (Netherlands)

van Ruissen, Fred; Schaaf, Gerben J.; Kool, Marcel; Baas, Frank; Ruijter, Jan M.

2008-01-01

Serial analysis of gene expression (SAGE) and microarrays have found a widespread application, but much ambiguity exists regarding the amalgamation of the data resulting from these technologies. Cross-platform utilization of gene expression data from the SAGE and microarray technology could reduce

MLS/Aura L2 Hydroxyl (OH) Mixing Ratio V002

Data.gov (United States)

National Aeronautics and Space Administration — ML2OH is the EOS Aura Microwave Limb Sounder (MLS) standard product for hydroxyl derived from radiances measured by the THz radiometer. The current version is 2.2....

Acoustic Calibration and Trials (HB0707, EK60)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Operational objectives are to: (1) calibrate the EK60 Scientific Sounder, (2) calibrate the ME70, (3) collect acoustic data during the transit, (4) evaluate and...

miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

Energy Technology Data Exchange (ETDEWEB)

McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk [Biomedical Sciences Research Institute, University of Ulster, Coleraine, Co. Derry BT52 1SA (United Kingdom); Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); Patel, Daksha, E-mail: d.patel@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom); McCance, Dennis J., E-mail: d.mccance@qub.ac.uk [Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen' s University Belfast, Belfast BT9 7BL (United Kingdom)

2014-01-05

A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.

miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

International Nuclear Information System (INIS)

McKenna, Declan J.; Patel, Daksha; McCance, Dennis J.

2014-01-01

A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes

Craniopharyngioma: Survivin expression and ultrastructure

Science.gov (United States)

ZHU, JIANG; YOU, CHAO

2015-01-01

The aim of the present study was to investigate the significance of survivin protein expression levels in craniopharyngioma. Tumor samples and clinical data were obtained from 50 patients with craniopharyngioma who were admitted to the West China Hospital of Sichuan University (Chengdu, China). The morphology of the craniopharyngioma samples was observed using optical and electron microscopes, and survivin expression was investigated in the samples by immunohistochemical analysis. The immunohistochemical results revealed survivin expression in all of the craniopharyngioma samples, but not in the healthy brain tissue samples. It was identified that survivin was expressed at a higher level in cases of the adamantinomatous type compared with those of the squamous-papillary type, in male patients compared with female patients, in children compared with adults and in recurrent cases compared with non-recurrent cases. Furthermore, no significant difference was detected in survivin expression levels among the tumors of different subtypes and different disease stages. The results of the present study indicate that survivin is significant in the development of craniopharyngioma, and that survivin protein expression levels are a meaningful indicator for assessing craniopharyngioma recurrence. PMID:25435936

A Robot with Complex Facial Expressions

Directory of Open Access Journals (Sweden)

J. Takeno

2009-08-01

Full Text Available The authors believe that the consciousness of humans basically originates from languages and their association-like flow of consciousness, and that feelings are generated accompanying respective languages. We incorporated artificial consciousness into a robot; achieved an association flow of language like flow of consciousness; and developed a robot called Kansei that expresses its feelings according to the associations occurring in the robot. To be able to fully communicate with humans, robots must be able to display complex expressions, such as a sense of being thrilled. We therefore added to the Kansei robot a device to express complex feelings through its facial expressions. The Kansei robot is actually an artificial skull made of aluminum, with servomotors built into it. The face is made of relatively soft polyethylene, which is formed to appear like a human face. Facial expressions are generated using 19 servomotors built into the skull, which pull metal wires attached to the facial “skin” to create expressions. The robot at present is capable of making six basic expressions as well as complex expressions, such as happiness and fear combined.

Sad Facial Expressions Increase Choice Blindness

Directory of Open Access Journals (Sweden)

Yajie Wang

2018-01-01

Full Text Available Previous studies have discovered a fascinating phenomenon known as choice blindness—individuals fail to detect mismatches between the face they choose and the face replaced by the experimenter. Although previous studies have reported a couple of factors that can modulate the magnitude of choice blindness, the potential effect of facial expression on choice blindness has not yet been explored. Using faces with sad and neutral expressions (Experiment 1 and faces with happy and neutral expressions (Experiment 2 in the classic choice blindness paradigm, the present study investigated the effects of facial expressions on choice blindness. The results showed that the detection rate was significantly lower on sad faces than neutral faces, whereas no significant difference was observed between happy faces and neutral faces. The exploratory analysis of verbal reports found that participants who reported less facial features for sad (as compared to neutral expressions also tended to show a lower detection rate of sad (as compared to neutral faces. These findings indicated that sad facial expressions increased choice blindness, which might have resulted from inhibition of further processing of the detailed facial features by the less attractive sad expressions (as compared to neutral expressions.

Sad Facial Expressions Increase Choice Blindness.

Science.gov (United States)

Wang, Yajie; Zhao, Song; Zhang, Zhijie; Feng, Wenfeng

2017-01-01

Previous studies have discovered a fascinating phenomenon known as choice blindness-individuals fail to detect mismatches between the face they choose and the face replaced by the experimenter. Although previous studies have reported a couple of factors that can modulate the magnitude of choice blindness, the potential effect of facial expression on choice blindness has not yet been explored. Using faces with sad and neutral expressions (Experiment 1) and faces with happy and neutral expressions (Experiment 2) in the classic choice blindness paradigm, the present study investigated the effects of facial expressions on choice blindness. The results showed that the detection rate was significantly lower on sad faces than neutral faces, whereas no significant difference was observed between happy faces and neutral faces. The exploratory analysis of verbal reports found that participants who reported less facial features for sad (as compared to neutral) expressions also tended to show a lower detection rate of sad (as compared to neutral) faces. These findings indicated that sad facial expressions increased choice blindness, which might have resulted from inhibition of further processing of the detailed facial features by the less attractive sad expressions (as compared to neutral expressions).

Measuring facial expression of emotion.

Science.gov (United States)

Wolf, Karsten

2015-12-01

Research into emotions has increased in recent decades, especially on the subject of recognition of emotions. However, studies of the facial expressions of emotion were compromised by technical problems with visible video analysis and electromyography in experimental settings. These have only recently been overcome. There have been new developments in the field of automated computerized facial recognition; allowing real-time identification of facial expression in social environments. This review addresses three approaches to measuring facial expression of emotion and describes their specific contributions to understanding emotion in the healthy population and in persons with mental illness. Despite recent progress, studies on human emotions have been hindered by the lack of consensus on an emotion theory suited to examining the dynamic aspects of emotion and its expression. Studying expression of emotion in patients with mental health conditions for diagnostic and therapeutic purposes will profit from theoretical and methodological progress.

Freedom of Expression

Directory of Open Access Journals (Sweden)

Guilherme Canela

2007-06-01

Full Text Available The freedoms of expression and of the press are basic pillars of the western democracies. The contemporary theoretical framework which gives support to these rights was generated in the wake of the liberal revolutions which took place in Western Europe and in North America starting from the second half of the 1600s. Our purpose in this text is to present the current scene regarding this topic, focusing whenever pertinent on the Brazilian case, and seeking to question the unconditional defense of the freedoms of expression and of the press made by the thinkers who founded these principles vis-á-vis contemporary issues of the communicational universe. Going beyond theoretical-conceptual refl ections, we present and analyze the results of a content analysis showing how 53 Brazilian newspapers and 4 magazines with nationwide circulation report (or not topics relating to freedom of expression and of the press.

Gene expression inference with deep learning.

Science.gov (United States)

Chen, Yifei; Li, Yi; Narayan, Rajiv; Subramanian, Aravind; Xie, Xiaohui

2016-06-15

Large-scale gene expression profiling has been widely used to characterize cellular states in response to various disease conditions, genetic perturbations, etc. Although the cost of whole-genome expression profiles has been dropping steadily, generating a compendium of expression profiling over thousands of samples is still very expensive. Recognizing that gene expressions are often highly correlated, researchers from the NIH LINCS program have developed a cost-effective strategy of profiling only ∼1000 carefully selected landmark genes and relying on computational methods to infer the expression of remaining target genes. However, the computational approach adopted by the LINCS program is currently based on linear regression (LR), limiting its accuracy since it does not capture complex nonlinear relationship between expressions of genes. We present a deep learning method (abbreviated as D-GEX) to infer the expression of target genes from the expression of landmark genes. We used the microarray-based Gene Expression Omnibus dataset, consisting of 111K expression profiles, to train our model and compare its performance to those from other methods. In terms of mean absolute error averaged across all genes, deep learning significantly outperforms LR with 15.33% relative improvement. A gene-wise comparative analysis shows that deep learning achieves lower error than LR in 99.97% of the target genes. We also tested the performance of our learned model on an independent RNA-Seq-based GTEx dataset, which consists of 2921 expression profiles. Deep learning still outperforms LR with 6.57% relative improvement, and achieves lower error in 81.31% of the target genes. D-GEX is available at https://github.com/uci-cbcl/D-GEX CONTACT: xhx@ics.uci.edu Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival

Directory of Open Access Journals (Sweden)

Lincoln Douglas

2006-06-01

Full Text Available Abstract Background Increased expression of Eph receptor tyrosine kinases and their ephrin ligands has been implicated in tumor progression in a number of malignancies. This report describes aberrant expression of these genes in ovarian cancer, the commonest cause of death amongst gynaecological malignancies. Methods Eph and ephrin expression was determined using quantitative real time RT-PCR. Correlation of gene expression was measured using Spearman's rho statistic. Survival was analysed using log-rank analysis and (was visualised by Kaplan-Meier survival curves. Results Greater than 10 fold over-expression of EphA1 and a more modest over-expression of EphA2 were observed in partially overlapping subsets of tumors. Over-expression of EphA1 strongly correlated (r = 0.801; p Conclusion These data imply that increased levels of ephrins A1 and A5 in the presence of high expression of Ephs A1 and A2 lead to a more aggressive tumor phenotype. The known functions of Eph/ephrin signalling in cell de-adhesion and movement may explain the observed correlation of ephrin expression with poor prognosis.

IceBridge HiCARS 2 L0 Raw Return Energy Amplitudes

Data.gov (United States)

National Aeronautics and Space Administration — The NASA IceBridge HiCARS 2 Level-0 Raw Return Energy Amplitudes (IR2HI0) data set contains radar sounder measurements taken over Antarctica using the Hi-Capability...

Elevation - Survey Points - Minnesota River Watershed Study Project

Data.gov (United States)

Army Corps of Engineers, Department of the Army, Department of Defense — Topograhic and hydrographic field located survey grade points collected using a Trimble GPS unit, Trimble robotic total stations, and/or Hydrolite-TM eco-sounder....

MLS/Aura Level 2 Nitrous Oxide (N2O) Mixing Ratio V004

Data.gov (United States)

National Aeronautics and Space Administration — ML2N2O is the EOS Aura Microwave Limb Sounder (MLS) standard product for nitrous oxide derived from radiances measured primarily by the 640 GHz radiometer (Band 12)...

Observation of wave generation and non-local perturbations in the ...

Indian Academy of Sciences (India)

in thermodynamics of lower atmosphere have the potential to trigger a spectrum of ..... exchange of trace constituents: The water vapor puzzle;. Terra Scientific ... Limb Sounder observations of gravity waves in the strato- sphere: A climatology ...

Equatorial wave activity during 2007 over Gadanki, a tropical station

Indian Academy of Sciences (India)

been used to investigate the wave activity in the troposphere and lower stratosphere. Waves in the ...... Oltmans S J 2001 Water vapor control at the tropopause by equatorial Kelvin .... observed in UARS microwave limb sounder temperature.

NESDIS Total Ozone from Analysis of Stratospheric and Tropospheric components (TOAST)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — TOAST combines UV and IR ozone retrievals from an algorithm using the Solar Backscatter Ultraviolet Version 2 (SBUV/2) and the Cross-track Infrared Sounder (CrIS)...

Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps

Directory of Open Access Journals (Sweden)

Überla Klaus

2007-06-01

Full Text Available Abstract Background Proteins of human and animal viruses are frequently expressed from RNA polymerase II dependent expression cassettes to study protein function and to develop gene-based vaccines. Initial attempts to express the G protein of vesicular stomatitis virus (VSV and the F protein of respiratory syncytial virus (RSV by eukaryotic promoters revealed restrictions at several steps of gene expression. Results Insertion of an intron flanked by exonic sequences 5'-terminal to the open reading frames (ORF of VSV-G and RSV-F led to detectable cytoplasmic mRNA levels of both genes. While the exonic sequences were sufficient to stabilise the VSV-G mRNA, cytoplasmic mRNA levels of RSV-F were dependent on the presence of a functional intron. Cytoplasmic VSV-G mRNA levels led to readily detectable levels of VSV-G protein, whereas RSV-F protein expression remained undetectable. However, RSV-F expression was observed after mutating two of four consensus sites for polyadenylation present in the RSV-F ORF. Expression levels could be further enhanced by codon optimisation. Conclusion Insufficient cytoplasmic mRNA levels and premature polyadenylation prevent expression of RSV-F by RNA polymerase II dependent expression plasmids. Since RSV replicates in the cytoplasm, the presence of premature polyadenylation sites and elements leading to nuclear instability should not interfere with RSV-F expression during virus replication. The molecular mechanisms responsible for the destabilisation of the RSV-F and VSV-G mRNAs and the different requirements for their rescue by insertion of an intron remain to be defined.

Nonverbal and verbal emotional expression and health.

Science.gov (United States)

Berry, D S; Pennebaker, J W

1993-01-01

The spontaneous nonverbal expression of emotion is related to immediate reductions in autonomic nervous system activity. Similar changes in specific autonomic channels occur when individuals are encouraged to verbally express their emotions. Indeed, these physiological changes are most likely to occur among individuals who are either verbally or nonverbally highly expressive. These data suggest that when individuals must actively inhibit emotional expression, they are at increased risk for a variety of health problems. Several experiments are summarized which indicate that verbally expressing traumatic experiences by writing or talking improves physical health, enhances immune function, and is associated with fewer medical visits. Although less research is available regarding nonverbal expression, it is also likely that the nonverbal expression of emotion bears some relation to health status. We propose that the effectiveness of many common expressive therapies (e.g., art, music, cathartic) would be enhanced if clients are encouraged to both express their feelings nonverbally and to put their experiences into words.

Precise Analysis of String Expressions

DEFF Research Database (Denmark)

Christensen, Aske Simon; Møller, Anders; Schwartzbach, Michael Ignatieff

2003-01-01

We perform static analysis of Java programs to answer a simple question: which values may occur as results of string expressions? The answers are summarized for each expression by a regular language that is guaranteed to contain all possible values. We present several applications of this analysis...... are automatically produced. We present extensive benchmarks demonstrating that the analysis is efficient and produces results of useful precision......., including statically checking the syntax of dynamically generated expressions, such as SQL queries. Our analysis constructs flow graphs from class files and generates a context-free grammar with a nonterminal for each string expression. The language of this grammar is then widened into a regular language...

Family emotional expressiveness and family structure

Directory of Open Access Journals (Sweden)

Čotar-Konrad Sonja

2016-01-01

Full Text Available The present paper scrutinizes the relationship between family emotional expressiveness (i.e., the tendency to express dominant and/or submissive positive and negative emotions and components of family structure as proposed in Olson’s Circumplex model (i.e., cohesion and flexibility, family communication, and satisfaction in families with adolescents. The study was conducted on a sample of 514 Slovenian adolescents, who filled out two questionnaires: the Slovenian version of Family Emotional Expressiveness - FEQ and FACES IV. The results revealed that all four basic dimensions of family functioning were significantly associated with higher/more frequent expressions of positive submissive emotions, as well as with lower/less frequent expressions of negative dominant emotions. Moreover, expressions of negative submissive emotions explained a small, but significant amount of variance in three out of four family functioning variables (satisfaction, flexibility, and communication. The importance of particular aspects of emotional expressiveness for family cohesion, flexibility, communication, and satisfaction is discussed, and the relevance of present findings for family counselling is outlined.

Widespread ectopic expression of olfactory receptor genes

Directory of Open Access Journals (Sweden)

Yanai Itai

2006-05-01

Full Text Available Abstract Background Olfactory receptors (ORs are the largest gene family in the human genome. Although they are expected to be expressed specifically in olfactory tissues, some ectopic expression has been reported, with special emphasis on sperm and testis. The present study systematically explores the expression patterns of OR genes in a large number of tissues and assesses the potential functional implication of such ectopic expression. Results We analyzed the expression of hundreds of human and mouse OR transcripts, via EST and microarray data, in several dozens of human and mouse tissues. Different tissues had specific, relatively small OR gene subsets which had particularly high expression levels. In testis, average expression was not particularly high, and very few highly expressed genes were found, none corresponding to ORs previously implicated in sperm chemotaxis. Higher expression levels were more common for genes with a non-OR genomic neighbor. Importantly, no correlation in expression levels was detected for human-mouse orthologous pairs. Also, no significant difference in expression levels was seen between intact and pseudogenized ORs, except for the pseudogenes of subfamily 7E which has undergone a human-specific expansion. Conclusion The OR superfamily as a whole, show widespread, locus-dependent and heterogeneous expression, in agreement with a neutral or near neutral evolutionary model for transcription control. These results cannot reject the possibility that small OR subsets might play functional roles in different tissues, however considerable care should be exerted when offering a functional interpretation for ectopic OR expression based only on transcription information.

Stochastic gene expression in Arabidopsis thaliana.

Science.gov (United States)

Araújo, Ilka Schultheiß; Pietsch, Jessica Magdalena; Keizer, Emma Mathilde; Greese, Bettina; Balkunde, Rachappa; Fleck, Christian; Hülskamp, Martin

2017-12-14

Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.

Docosahexaenoic acid inhibits IL-6 expression via PPARγ-mediated expression of catalase in cerulein-stimulated pancreatic acinar cells.

Science.gov (United States)

Song, Eun Ah; Lim, Joo Weon; Kim, Hyeyoung

2017-07-01

Cerulein pancreatitis mirrors human acute pancreatitis. In pancreatic acinar cells exposed to cerulein, reactive oxygen species (ROS) mediate inflammatory signaling by Janus kinase (JAK) 2/signal transducer and activator of transcription (STAT) 3, and cytokine induction. Docosahexaenoic acid (DHA) acts as an agonist of peroxisome proliferator activated receptor γ (PPARγ), which mediates the expression of some antioxidant enzymes. We hypothesized that DHA may induce PPARγ-target catalase expression and reduce ROS levels, leading to the inhibition of JAK2/STAT3 activation and IL-6 expression in cerulein-stimulated acinar cells. Pancreatic acinar AR42J cells were treated with DHA in the presence or absence of the PPARγ antagonist GW9662, or treated with the PPARγ agonist troglitazone, and then stimulated with cerulein. Expression of IL-6 and catalase, ROS levels, JAK2/STAT3 activation, and nuclear translocation of PPARγ were assessed. DHA suppressed the increase in ROS, JAK2/STAT3 activation, and IL-6 expression induced nuclear translocation of PPARγ and catalase expression in cerulein-stimulated AR42J cells. Troglitazone inhibited the cerulein-induced increase in ROS and IL-6 expression, but induced catalase expression similar to DHA in AR42J cells. GW9662 abolished the inhibitory effect of DHA on cerulein-induced increase in ROS and IL-6 expression in AR42J cells. DHA-induced expression of catalase was suppressed by GW9662 in cerulein-stimulated AR42J cells. Thus, DHA induces PPARγ activation and catalase expression, which inhibits ROS-mediated activation of JAK2/STAT3 and IL-6 expression in cerulein-stimulated pancreatic acinar cells. Copyright © 2017. Published by Elsevier Ltd.

Recognition, Expression, and Understanding Facial Expressions of Emotion in Adolescents with Nonverbal and General Learning Disabilities

Science.gov (United States)

Bloom, Elana; Heath, Nancy

2010-01-01

Children with nonverbal learning disabilities (NVLD) have been found to be worse at recognizing facial expressions than children with verbal learning disabilities (LD) and without LD. However, little research has been done with adolescents. In addition, expressing and understanding facial expressions is yet to be studied among adolescents with LD…

Recombination-ready Sindbis replicon expression vectors for transgene expression

Directory of Open Access Journals (Sweden)

Olson Ken E

2007-10-01

Full Text Available Abstract Background Sindbis viruses have been widely used as tools to study gene function in cells. Despite the utility of these systems, the construction and production of alphavirus replicons is time consuming and inefficient due to potential additional restriction sites within the insert region and lack of directionality for insert ligation. In this report, we present a system useful for producing recombinant Sindbis replicons that uses lambda phage recombination technology to rapidly and specifically construct replicon expression plasmids that contain insert regions in the desired orientation. Results Recombination of the gene of interest with the replicon plasmid resulted in nearly 100% recombinants, each of which contained a correctly orientated insert. Replicons were easily produced in cell culture and packaged into pseudo-infectious viral particles. Insect and mammalian cells infected with pseudo-infectious viral particles expressed various transgenes at high levels. Finally, inserts from persistently replicating replicon RNA were easily isolated and recombined back into entry plasmids for sequencing and subsequent analysis. Conclusion Replication-ready replicon expression plasmids make the use of alphavirus replicons fast and easy as compared to traditional replicon production methods. This system represents a significant step forward in the utility and ease of use of alphavirus replicons in the study of gene function.

Digital gene expression analysis of gene expression differences within Brassica diploids and allopolyploids.

Science.gov (United States)

Jiang, Jinjin; Wang, Yue; Zhu, Bao; Fang, Tingting; Fang, Yujie; Wang, Youping

2015-01-27

Brassica includes many successfully cultivated crop species of polyploid origin, either by ancestral genome triplication or by hybridization between two diploid progenitors, displaying complex repetitive sequences and transposons. The U's triangle, which consists of three diploids and three amphidiploids, is optimal for the analysis of complicated genomes after polyploidization. Next-generation sequencing enables the transcriptome profiling of polyploids on a global scale. We examined the gene expression patterns of three diploids (Brassica rapa, B. nigra, and B. oleracea) and three amphidiploids (B. napus, B. juncea, and B. carinata) via digital gene expression analysis. In total, the libraries generated between 5.7 and 6.1 million raw reads, and the clean tags of each library were mapped to 18547-21995 genes of B. rapa genome. The unambiguous tag-mapped genes in the libraries were compared. Moreover, the majority of differentially expressed genes (DEGs) were explored among diploids as well as between diploids and amphidiploids. Gene ontological analysis was performed to functionally categorize these DEGs into different classes. The Kyoto Encyclopedia of Genes and Genomes analysis was performed to assign these DEGs into approximately 120 pathways, among which the metabolic pathway, biosynthesis of secondary metabolites, and peroxisomal pathway were enriched. The non-additive genes in Brassica amphidiploids were analyzed, and the results indicated that orthologous genes in polyploids are frequently expressed in a non-additive pattern. Methyltransferase genes showed differential expression pattern in Brassica species. Our results provided an understanding of the transcriptome complexity of natural Brassica species. The gene expression changes in diploids and allopolyploids may help elucidate the morphological and physiological differences among Brassica species.

MLH1 expression predicts the response to preoperative therapy and is associated with PD-L1 expression in esophageal cancer.

Science.gov (United States)

Momose, Kota; Yamasaki, Makoto; Tanaka, Koji; Miyazaki, Yasuhiro; Makino, Tomoki; Takahashi, Tsuyoshi; Kurokawa, Yukinori; Nakajima, Kiyokazu; Takiguchi, Shuji; Mori, Masaki; Doki, Yuichiro

2017-07-01

Programmed death-ligand 1 (PD-1/PD-L1) inhibition therapy demonstrates potential as a future treatment for esophageal cancer. Mismatch repair status and tumor PD-L1 expression are the candidate predictive biomarkers for response to this therapy. In colorectal cancer, mismatch repair-deficient tumors are associated with improved survival, although they are not sensitive to 5-fluorouracil-based chemotherapy. The purpose of the present study was to investigate the association between MutL homolog 1 (MLH1) expression and prognosis, response to therapy and PD-L1 expression in esophageal cancer. Immunohistochemistry was used to evaluate MLH1 and PD-L1 expression in 251 resected specimens. Of the specimens, 30.3% exhibited low MLH1 expression and 15.5% exhibited high PD-L1 expression. The 5-year overall survival rates for the high MLH1 expression group and the low MLH1 expression group were 51.3 and 55.6%, respectively (P=0.5260). The responder ratio was 45.7% in the high MLH1 expression group and 15.4% in the low MLH1 expression group (PMLH1 expression group (P=0.0064) and 25.0% in the low MLH1 expression group. MLH1 expression may be a predictive factor for the response to preoperative therapy in esophageal cancer, and esophageal cancer with low MLH1 expression may have a mechanism that assists in promoting tumor PD-L1 expression.

Reconstructing dynamic mental models of facial expressions in prosopagnosia reveals distinct representations for identity and expression.

Science.gov (United States)

Richoz, Anne-Raphaëlle; Jack, Rachael E; Garrod, Oliver G B; Schyns, Philippe G; Caldara, Roberto

2015-04-01

The human face transmits a wealth of signals that readily provide crucial information for social interactions, such as facial identity and emotional expression. Yet, a fundamental question remains unresolved: does the face information for identity and emotional expression categorization tap into common or distinct representational systems? To address this question we tested PS, a pure case of acquired prosopagnosia with bilateral occipitotemporal lesions anatomically sparing the regions that are assumed to contribute to facial expression (de)coding (i.e., the amygdala, the insula and the posterior superior temporal sulcus--pSTS). We previously demonstrated that PS does not use information from the eye region to identify faces, but relies on the suboptimal mouth region. PS's abnormal information use for identity, coupled with her neural dissociation, provides a unique opportunity to probe the existence of a dichotomy in the face representational system. To reconstruct the mental models of the six basic facial expressions of emotion in PS and age-matched healthy observers, we used a novel reverse correlation technique tracking information use on dynamic faces. PS was comparable to controls, using all facial features to (de)code facial expressions with the exception of fear. PS's normal (de)coding of dynamic facial expressions suggests that the face system relies either on distinct representational systems for identity and expression, or dissociable cortical pathways to access them. Interestingly, PS showed a selective impairment for categorizing many static facial expressions, which could be accounted for by her lesion in the right inferior occipital gyrus. PS's advantage for dynamic facial expressions might instead relate to a functionally distinct and sufficient cortical pathway directly connecting the early visual cortex to the spared pSTS. Altogether, our data provide critical insights on the healthy and impaired face systems, question evidence of deficits

SOUND VELOCITY and Other Data from FIXED PLATFORM and Other Platforms From NE Atlantic (limit-40 W) and Others from 19860512 to 19891112 (NODC Accession 9000121)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The sound velocity and other data in this accession has been processed by NODC from a tape submitted by the originator containing Inverted Echo Sounder data from the...

The Financial Turmoil and the Lessons from the Emerging World

International Development Research Centre (IDRC) Digital Library (Canada)

bbattisti

are comparable to those that followed the 1929 stock market crash. ... credit crunch in advanced economies so as to dampen the effects on the global economy ... The strategy based on sounder macro fundamentals and domestic institutions ...

Anger expression among Danish cyclists and drivers: A comparison based on mode specific anger expression inventories

DEFF Research Database (Denmark)

Møller, Mette; Haustein, Sonja

2017-01-01

, gender, self-reported aggressive behaviours and traffic fines: Women scored for instance lower in physical expression, while older people scored higher in constructive expression. The effect of age and gender on anger expression among drivers and cyclists remained significant when controlling......Based on the short form of the driving anger expression inventory (DAX-short, 15-item), the present study developed an adapted version of the DAX for cyclists (CAX, 14 items). The data basis was an online survey of 2000 inhabitants of Denmark. A principle component analysis on the translated DAX...... for exposure and other factors in linear regression analyses. These analyses also showed a relationship between a positive attitude towards driving and higher levels of anger expression among drivers, while this was not the case for cyclists....

Vitamin D Receptor Expression in Dogs

Science.gov (United States)

Gow, A.G.; Milne, E.; Drummond, D.; Smith, S.; Handel, I.; Mellanby, R.J.

2018-01-01

Background There is growing evidence linking low blood vitamin D concentration to numerous diseases in people and in dogs. Vitamin D influences cellular function by signaling through the vitamin D receptor (VDR). Little is known about which non‐skeletal tissues express the VDR or how inflammation influences its expression in the dog. Objectives To define which non‐skeletal canine tissues express the VDR and to investigate expression in inflamed small intestine. Animals Thirteen non‐skeletal tissues were collected prospectively from 6 control dogs. Thirty‐five dogs diagnosed with a chronic enteropathy (CE) and 24 control dogs were prospectively enrolled and duodenal biopsies were evaluated for VDR expression. Methods Prospective; blinded assessment of canine intestinal VDR. Dogs with CE were included once other identifiable causes of intestinal disease were excluded. Age matched controls were included with no intestinal clinical signs. VDR expression was assessed immunohistochemically in all samples, using a Rat IgG VDR monoclonal antibody. Quantitative real‐time polymerase chain reaction (qPCR) was also used for duodenal biopsies. Results VDR expression as assessed by immunohistochemistry (IHC) was highest in the kidney, duodenum, skin, ileum and spleen, and weak in the colon, heart, lymph node, liver, lung, and ovary. Gastric and testicular tissue did not express the VDR. There was no statistical difference in duodenal VDR expression between the 24 healthy dogs and 34 dogs with CE when quantified by either qPCR (P = 0.87) or IHC (P = 0.099). Conclusions and Clinical Importance The lack of down regulation of VDR expression in inflamed intestine contrasts with previous studies in humans. Our findings support future studies to investigate whether vitamin D and its analogues can be used to modulate intestinal inflammation in the dog. PMID:29469965

VH gene expression and regulation in the mutant Alicia rabbit. Rescue of VHa2 allotype expression.

Science.gov (United States)

Chen, H T; Alexander, C B; Young-Cooper, G O; Mage, R G

1993-04-01

Rabbits of the Alicia strain, derived from rabbits expressing the VHa2 allotype, have a mutation in the H chain locus that has a cis effect upon the expression of VHa2 and VHa- genes. A small deletion at the most J-proximal (3') end of the VH locus leads to low expression of all the genes on the entire chromosome in heterozygous ali mutants and altered relative expression of VH genes in homozygotes. To study VH gene expression and regulation, we used the polymerase chain reaction to amplify the VH genes expressed in spleens of young and adult wild-type and mutant Alicia rabbits. The cDNA from reverse transcription of splenic mRNA was amplified and polymerase chain reaction libraries were constructed and screened with oligonucleotides from framework regions 1 and 3, as well as JH. Thirty-three VH-positive clones were sequenced and analyzed. We found that in mutant Alicia rabbits, products of the first functional VH gene (VH4a2), (or VH4a2-like genes) were expressed in 2- to 8-wk-olds. Expression of both the VHx and VHy types of VHa- genes was also elevated but the relative proportions of VHx and VHy, especially VHx, decreased whereas the relative levels of expression of VH4a2 or VH4a2-like genes increased with age. Our results suggest that the appearance of sequences resembling that of the VH1a2, which is deleted in the mutant ali rabbits, could be caused by alterations of the sequences of the rearranged VH4a2 genes by gene conversions and/or rearrangement of upstream VH1a2-like genes later in development.

Emotion expression, decision-making and well-being.

Science.gov (United States)

Xiao, Erte

2008-01-01

This chapter discusses the role of emotion expression in decision-making. To understand connections between emotion and decision it is helpful first to differentiate between emotion experience and emotion expression. Understanding how emotion expression influences decision-making is important as a practical matter. However, in contrast to emotion experience, economic research has paid little attention to the significance of emotion expression in decision-making. I review recent studies on emotion expression, paying specific attention to possible connections between emotion expression, punishment, fair economic exchange, and well-being. In contrast to emotions, which are typically difficult to control, I suggest that opportunities for emotion expression can feasibly be manipulated through appropriately designed policies. I further suggest that this approach may have the ability to positively affect well-being and economic outcomes. VALUE OF THE CHAPTER: The chapter provides new perspectives on how policy-makers can benefit by understanding the effect of emotion expression in decision-making. The chapter also suggests future research to improve our understanding of emotion expression.

Generating Expressive Speech for Storytelling Applications

NARCIS (Netherlands)

Bailly, G.; Theune, Mariet; Meijs, Koen; Campbell, N.; Hamza, W.; Heylen, Dirk K.J.; Ordelman, Roeland J.F.; Hoge, H.; Jianhua, T.

Work on expressive speech synthesis has long focused on the expression of basic emotions. In recent years, however, interest in other expressive styles has been increasing. The research presented in this paper aims at the generation of a storytelling speaking style, which is suitable for

Darwin and Emotion Expression

Science.gov (United States)

Hess, Ursula; Thibault, Pascal

2009-01-01

In his book "The Expression of the Emotions in Man and Animals," Charles Darwin (1872/1965) defended the argument that emotion expressions are evolved and adaptive (at least at some point in the past) and serve an important communicative function. The ideas he developed in his book had an important impact on the field and spawned rich domains of…

Comparative gene expression between two yeast species

Directory of Open Access Journals (Sweden)

Guan Yuanfang

2013-01-01

Full Text Available Abstract Background Comparative genomics brings insight into sequence evolution, but even more may be learned by coupling sequence analyses with experimental tests of gene function and regulation. However, the reliability of such comparisons is often limited by biased sampling of expression conditions and incomplete knowledge of gene functions across species. To address these challenges, we previously systematically generated expression profiles in Saccharomyces bayanus to maximize functional coverage as compared to an existing Saccharomyces cerevisiae data repository. Results In this paper, we take advantage of these two data repositories to compare patterns of ortholog expression in a wide variety of conditions. First, we developed a scalable metric for expression divergence that enabled us to detect a significant correlation between sequence and expression conservation on the global level, which previous smaller-scale expression studies failed to detect. Despite this global conservation trend, between-species gene expression neighborhoods were less well-conserved than within-species comparisons across different environmental perturbations, and approximately 4% of orthologs exhibited a significant change in co-expression partners. Furthermore, our analysis of matched perturbations collected in both species (such as diauxic shift and cell cycle synchrony demonstrated that approximately a quarter of orthologs exhibit condition-specific expression pattern differences. Conclusions Taken together, these analyses provide a global view of gene expression patterns between two species, both in terms of the conditions and timing of a gene's expression as well as co-expression partners. Our results provide testable hypotheses that will direct future experiments to determine how these changes may be specified in the genome.

Regular Expression Matching and Operational Semantics

Directory of Open Access Journals (Sweden)

Asiri Rathnayake

2011-08-01

Full Text Available Many programming languages and tools, ranging from grep to the Java String library, contain regular expression matchers. Rather than first translating a regular expression into a deterministic finite automaton, such implementations typically match the regular expression on the fly. Thus they can be seen as virtual machines interpreting the regular expression much as if it were a program with some non-deterministic constructs such as the Kleene star. We formalize this implementation technique for regular expression matching using operational semantics. Specifically, we derive a series of abstract machines, moving from the abstract definition of matching to increasingly realistic machines. First a continuation is added to the operational semantics to describe what remains to be matched after the current expression. Next, we represent the expression as a data structure using pointers, which enables redundant searches to be eliminated via testing for pointer equality. From there, we arrive both at Thompson's lockstep construction and a machine that performs some operations in parallel, suitable for implementation on a large number of cores, such as a GPU. We formalize the parallel machine using process algebra and report some preliminary experiments with an implementation on a graphics processor using CUDA.

DNA expressions - A formal notation for DNA

NARCIS (Netherlands)

Vliet, Rudy van

2015-01-01

We describe a formal notation for DNA molecules that may contain nicks and gaps. The resulting DNA expressions denote formal DNA molecules. Different DNA expressions may denote the same molecule. Such DNA expressions are called equivalent. We examine which DNA expressions are minimal, which

S-NPP ATMS Instrument Prelaunch and On-Orbit Performance Evaluation

Science.gov (United States)

Kim, Edward; Lyu, Cheng-Hsuan; Anderson, Kent; Leslie, Vincent R.; Blackwell, William J.

2014-01-01

The first of a new generation of microwave sounders was launched aboard the Suomi-National Polar-Orbiting Partnership satellite in October 2011. The Advanced Technology Microwave Sounder (ATMS) combines the capabilities and channel sets of three predecessor sounders into a single package to provide information on the atmospheric vertical temperature and moisture profiles that are the most critical observations needed for numerical weather forecast models. Enhancements include size/mass/power approximately one third of the previous total, three new sounding channels, the first space-based, Nyquist-sampled cross-track microwave temperature soundings for improved fusion with infrared soundings, plus improved temperature control and reliability. This paper describes the ATMS characteristics versus its predecessor, the advanced microwave sounding unit (AMSU), and presents the first comprehensive evaluation of key prelaunch and on-orbit performance parameters. Two-year on-orbit performance shows that the ATMS has maintained very stable radiometric sensitivity, in agreement with prelaunch data, meeting requirements for all channels (with margins of 40% for channels 1-15), and improvements over AMSU-A when processed for equivalent spatial resolution. The radiometric accuracy, determined by analysis from ground test measurements, and using on-orbit instrument temperatures, also shows large margins relative to requirements (specified as ATMS is especially important for this first proto-flight model unit of what will eventually be a series of ATMS sensors providing operational sounding capability for the U.S. and its international partners well into the next decade.

An Observational Study of the Kuroshio in the East China Sea: Local, Regional, and Basin-Wide Perspectives on a Western Boundary Current

National Research Council Canada - National Science Library

Andres, Magdalena

2008-01-01

.... An observational study of the Kuroshio was conducted using data collected in the East China Sea (ECS) north of Okinawa from December 2002 through November 2004 with an array of inverted echo sounders and acoustic Doppler current profilers...

MLS/Aura L2 Nitric Acid (HNO3) Mixing Ratio V003

Data.gov (United States)

National Aeronautics and Space Administration — ML2HNO3 is the EOS Aura Microwave Limb Sounder (MLS) standard product for nitric acid derived from radiances measured by the 240 GHz radiometer at and below 10 hPa,...

MLS/Aura Level 2 Nitric Acid (HNO3) Mixing Ratio V004

Data.gov (United States)

National Aeronautics and Space Administration — ML2HNO3 is the EOS Aura Microwave Limb Sounder (MLS) standard product for nitric acid derived from radiances measured by the 240 GHz radiometer at and below 10 hPa,...

MLS/Aura L2 Nitric Acid (HNO3) Mixing Ratio V002

Data.gov (United States)

National Aeronautics and Space Administration — ML2HNO3 is the EOS Aura Microwave Limb Sounder (MLS) standard product for nitric acid derived from radiances measured by the 240 GHz radiometer at and below 10 hPa,...

MLS/Aura Level 2 Nitric Acid (HNO3) Mixing Ratio V003 (ML2HNO3) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — ML2HNO3 is the EOS Aura Microwave Limb Sounder (MLS) standard product for nitric acid derived from radiances measured by the 240 GHz radiometer at and below 10 hPa,...

MLS/Aura Level 2 Nitric Acid (HNO3) Mixing Ratio V004 (ML2HNO3) at GES DISC

Data.gov (United States)

National Aeronautics and Space Administration — ML2HNO3 is the EOS Aura Microwave Limb Sounder (MLS) standard product for nitric acid derived from radiances measured by the 240 GHz radiometer at and below 10 hPa,...

Extracting gene expression patterns and identifying co-expressed genes from microarray data reveals biologically responsive processes

Directory of Open Access Journals (Sweden)

Paules Richard S

2007-11-01

Full Text Available Abstract Background A common observation in the analysis of gene expression data is that many genes display similarity in their expression patterns and therefore appear to be co-regulated. However, the variation associated with microarray data and the complexity of the experimental designs make the acquisition of co-expressed genes a challenge. We developed a novel method for Extracting microarray gene expression Patterns and Identifying co-expressed Genes, designated as EPIG. The approach utilizes the underlying structure of gene expression data to extract patterns and identify co-expressed genes that are responsive to experimental conditions. Results Through evaluation of the correlations among profiles, the magnitude of variation in gene expression profiles, and profile signal-to-noise ratio's, EPIG extracts a set of patterns representing co-expressed genes. The method is shown to work well with a simulated data set and microarray data obtained from time-series studies of dauer recovery and L1 starvation in C. elegans and after ultraviolet (UV or ionizing radiation (IR-induced DNA damage in diploid human fibroblasts. With the simulated data set, EPIG extracted the appropriate number of patterns which were more stable and homogeneous than the set of patterns that were determined using the CLICK or CAST clustering algorithms. However, CLICK performed better than EPIG and CAST with respect to the average correlation between clusters/patterns of the simulated data. With real biological data, EPIG extracted more dauer-specific patterns than CLICK. Furthermore, analysis of the IR/UV data revealed 18 unique patterns and 2661 genes out of approximately 17,000 that were identified as significantly expressed and categorized to the patterns by EPIG. The time-dependent patterns displayed similar and dissimilar responses between IR and UV treatments. Gene Ontology analysis applied to each pattern-related subset of co-expressed genes revealed underlying

Neighboring Genes Show Correlated Evolution in Gene Expression

Science.gov (United States)

Ghanbarian, Avazeh T.; Hurst, Laurence D.

2015-01-01

When considering the evolution of a gene’s expression profile, we commonly assume that this is unaffected by its genomic neighborhood. This is, however, in contrast to what we know about the lack of autonomy between neighboring genes in gene expression profiles in extant taxa. Indeed, in all eukaryotic genomes genes of similar expression-profile tend to cluster, reflecting chromatin level dynamics. Does it follow that if a gene increases expression in a particular lineage then the genomic neighbors will also increase in their expression or is gene expression evolution autonomous? To address this here we consider evolution of human gene expression since the human-chimp common ancestor, allowing for both variation in estimation of current expression level and error in Bayesian estimation of the ancestral state. We find that in all tissues and both sexes, the change in gene expression of a focal gene on average predicts the change in gene expression of neighbors. The effect is highly pronounced in the immediate vicinity (genes increasing their expression in humans tend to avoid nuclear lamina domains and be enriched for the gene activator 5-hydroxymethylcytosine, we conclude that, most probably owing to chromatin level control of gene expression, a change in gene expression of one gene likely affects the expression evolution of neighbors, what we term expression piggybacking, an analog of hitchhiking. PMID:25743543

Impaired detection of happy facial expressions in autism.

Science.gov (United States)

Sato, Wataru; Sawada, Reiko; Uono, Shota; Yoshimura, Sayaka; Kochiyama, Takanori; Kubota, Yasutaka; Sakihama, Morimitsu; Toichi, Motomi

2017-10-17

The detection of emotional facial expressions plays an indispensable role in social interaction. Psychological studies have shown that typically developing (TD) individuals more rapidly detect emotional expressions than neutral expressions. However, it remains unclear whether individuals with autistic phenotypes, such as autism spectrum disorder (ASD) and high levels of autistic traits (ATs), are impaired in this ability. We examined this by comparing TD and ASD individuals in Experiment 1 and individuals with low and high ATs in Experiment 2 using the visual search paradigm. Participants detected normal facial expressions of anger and happiness and their anti-expressions within crowds of neutral expressions. In Experiment 1, reaction times were shorter for normal angry expressions than for anti-expressions in both TD and ASD groups. This was also the case for normal happy expressions vs. anti-expressions in the TD group but not in the ASD group. Similarly, in Experiment 2, the detection of normal vs. anti-expressions was faster for angry expressions in both groups and for happy expressions in the low, but not high, ATs group. These results suggest that the detection of happy facial expressions is impaired in individuals with ASD and high ATs, which may contribute to their difficulty in creating and maintaining affiliative social relationships.

Athero Express : ATHERO-sclerotic plaque EXPRESSion in relation to vascular events and patient characteristics

NARCIS (Netherlands)

Verhoeven, B.A.N.

2006-01-01

Athero-Express is a tissue bank study, designed to investigate the expression of atherosclerotic derived biological variables in relation to the long-term outcome of patients undergoing carotid endarterectomy. Its design includes both cross-sectional and follow-up studies, the results from which

eXpression2Kinases (X2K) Web: linking expression signatures to upstream cell signaling networks.

Science.gov (United States)

Clarke, Daniel J B; Kuleshov, Maxim V; Schilder, Brian M; Torre, Denis; Duffy, Mary E; Keenan, Alexandra B; Lachmann, Alexander; Feldmann, Axel S; Gundersen, Gregory W; Silverstein, Moshe C; Wang, Zichen; Ma'ayan, Avi

2018-05-25

While gene expression data at the mRNA level can be globally and accurately measured, profiling the activity of cell signaling pathways is currently much more difficult. eXpression2Kinases (X2K) computationally predicts involvement of upstream cell signaling pathways, given a signature of differentially expressed genes. X2K first computes enrichment for transcription factors likely to regulate the expression of the differentially expressed genes. The next step of X2K connects these enriched transcription factors through known protein-protein interactions (PPIs) to construct a subnetwork. The final step performs kinase enrichment analysis on the members of the subnetwork. X2K Web is a new implementation of the original eXpression2Kinases algorithm with important enhancements. X2K Web includes many new transcription factor and kinase libraries, and PPI networks. For demonstration, thousands of gene expression signatures induced by kinase inhibitors, applied to six breast cancer cell lines, are provided for fetching directly into X2K Web. The results are displayed as interactive downloadable vector graphic network images and bar graphs. Benchmarking various settings via random permutations enabled the identification of an optimal set of parameters to be used as the default settings in X2K Web. X2K Web is freely available from http://X2K.cloud.

Identifying Regulatory Patterns at the 3'end Regions of Over-expressed and Under-expressed Genes

KAUST Repository

Othoum, Ghofran K

2013-05-01

Promoters, neighboring regulatory regions and those extending further upstream of the 5’end of genes, are considered one of the main components affecting the expression status of genes in a specific phenotype. More recently research by Chen et al. (2006, 2012) and Mapendano et al. (2010) demonstrated that the 3’end regulatory regions of genes also influence gene expression. However, the association between the regulatory regions surrounding 3’end of genes and their over- or under-expression status in a particular phenotype has not been systematically studied. The aim of this study is to ascertain if regulatory regions surrounding the 3’end of genes contain sufficient regulatory information to correlate genes with their expression status in a particular phenotype. Over- and under-expressed ovarian cancer (OC) genes were used as a model. Exploratory analysis of the 3’end regions were performed by transforming the annotated regions using principal component analysis (PCA), followed by clustering the transformed data thereby achieving a clear separation of genes with different expression status. Additionally, several classification algorithms such as Naïve Bayes, Random Forest and Support Vector Machine (SVM) were tested with different parameter settings to analyze the discriminatory capacity of the 3’end regions of genes related to their gene expression status. The best performance was achieved using the SVM classification model with 10-fold cross-validation that yielded an accuracy of 98.4%, sensitivity of 99.5% and specificity of 92.5%. For gene expression status for newly available instances, based on information derived from the 3’end regions, an SVM predictive model was developed with 10-fold cross-validation that yielded an accuracy of 67.0%, sensitivity of 73.2% and specificity of 61.0%. Moreover, building an SVM with polynomial kernel model to PCA transformed data yielded an accuracy of 83.1%, sensitivity of 92.5% and specificity of 74.8% using

Identifying Regulatory Patterns at the 3'end Regions of Over-expressed and Under-expressed Genes

KAUST Repository

Othoum, Ghofran K

2013-01-01

Promoters, neighboring regulatory regions and those extending further upstream of the 5’end of genes, are considered one of the main components affecting the expression status of genes in a specific phenotype. More recently research by Chen et al. (2006, 2012) and Mapendano et al. (2010) demonstrated that the 3’end regulatory regions of genes also influence gene expression. However, the association between the regulatory regions surrounding 3’end of genes and their over- or under-expression status in a particular phenotype has not been systematically studied. The aim of this study is to ascertain if regulatory regions surrounding the 3’end of genes contain sufficient regulatory information to correlate genes with their expression status in a particular phenotype. Over- and under-expressed ovarian cancer (OC) genes were used as a model. Exploratory analysis of the 3’end regions were performed by transforming the annotated regions using principal component analysis (PCA), followed by clustering the transformed data thereby achieving a clear separation of genes with different expression status. Additionally, several classification algorithms such as Naïve Bayes, Random Forest and Support Vector Machine (SVM) were tested with different parameter settings to analyze the discriminatory capacity of the 3’end regions of genes related to their gene expression status. The best performance was achieved using the SVM classification model with 10-fold cross-validation that yielded an accuracy of 98.4%, sensitivity of 99.5% and specificity of 92.5%. For gene expression status for newly available instances, based on information derived from the 3’end regions, an SVM predictive model was developed with 10-fold cross-validation that yielded an accuracy of 67.0%, sensitivity of 73.2% and specificity of 61.0%. Moreover, building an SVM with polynomial kernel model to PCA transformed data yielded an accuracy of 83.1%, sensitivity of 92.5% and specificity of 74.8% using

Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis.

Science.gov (United States)

Linares, Daniel M; Alvarez-Sieiro, Patricia; del Rio, Beatriz; Ladero, Victor; Redruello, Begoña; Martin, Ma Cruz; Fernandez, Maria; Alvarez, Miguel A

2015-12-30

Lactococcus lactis has been safely consumed in fermented foods for millennia. This Gram-positive bacterium has now become of industrial importance as an expression host for the overproduction of lipopolysaccharide-free recombinant proteins used as food ingredients, therapeutic proteins and biotechnological enzymes. This paper reports an agmatine-controlled expression (ACE) system for L. lactis, comprising the lactococcal agmatine-sensor/transcriptional activator AguR and its target promoter P(aguB). The usefulness and efficiency of this system was checked via the reporter gene gfp and by producing PEP (Myxococcus xanthus prolyl-endopeptidase), an enzyme of biomedical interest able to degrade the immunotoxic peptides produced during the gastrointestinal breakdown of gluten. The ACE system developed in this work was suitable for the efficient expression of the functional recombinant proteins GFP and PEP. The expression system was tightly regulated by the agmatine concentration and allowed high protein production without leakiness.

Early results from the Whisper instrument on Cluster: An overview

DEFF Research Database (Denmark)

Decreau, P.M.E.; Fergeau, P.; Krasnoselskikh, V.

2001-01-01

The Whisper instrument yields two data sets: (i) the electron density determined via the relaxation sounder, and (ii) the spectrum of natural plasma emissions in the frequency band 2-80 kHz. Both data sets allow for the three-dimensional exploration of the magnetosphere by the Cluster mission...... the drift velocity of density structures. Wave observations are also of crucial interest for studying small-scale structures, as demonstrated in an example in the fore-shock region. Early results from the Whisper instrument are very encouraging, and demonstrate that the four-point Cluster measurements...... largely overcomes the limited telemetry allocation. The natural emissions are usually related to the plasma frequency, as identified by the sounder, and the combination of an active sounding operation and a passive survey operation provides a time resolution for the total density determination of 2.2 s...

Spatial distribution maps for benthic communities

DEFF Research Database (Denmark)

Sørensen, Per S.

1999-01-01

ecosystems, were selected. These species are supposed to be good indicators of marine ecosystem health. The hydroacoustic measurements comprise preprocessed echo sounder recordings and side-scan sonar data forming a large and unique collection of datasets based on 4 field campaigns in Øresund...... of the distribution maps and to be combined with biogeochemical models describing spatiotemporal population dynamics. Finally, the use of side-scan sonar data is illustrated in a data fusion exercise combining side-scan sonar data with the results based on echo sounder measurements. The feasible use of side......-scan sonar for mapping of benthic communities remains an open task to be studied in the future. The data processing methodology developed is a contribution to the emerging field of hydroacoustic marine biology. The method of penalised maximum pseudo-likelihood for estimation of the Ising model under a huge...

Implementation of the Land, Atmosphere Near Real-Time Capability for EOS (LANCE)

Science.gov (United States)

Michael, Karen; Murphy, Kevin; Lowe, Dawn; Masuoka, Edward; Vollmer, Bruce; Tilmes, Curt; Teague, Michael; Ye, Gang; Maiden, Martha; Goodman, H. Michael;

Personality expression in Chinese language use.

Science.gov (United States)

Qiu, Lin; Lu, Jiahui; Ramsay, Jonathan; Yang, Shanshan; Qu, Weina; Zhu, Tingshao

2017-12-01

To date, little research has investigated personality expressions in languages other than English. Given that the Chinese language has the largest number of native speakers in the world, it is vitally important to examine the associations between personality and Chinese language use. In this research, we analysed Chinese microblogs and identified word categories and factorial structures associated with personality traits. We also compared our results with previous findings in English and showed that linguistic expression of personality has both universal- and language-specific aspects. Expression of personality via content words is more likely to be consistent across languages than expression via function words. This makes an important step towards uncovering universal patterns of personality expression in language. © 2016 International Union of Psychological Science.

Examining emotional expressions in discourse: methodological considerations

Science.gov (United States)

Hufnagel, Elizabeth; Kelly, Gregory J.

2017-10-01

This methodological paper presents an approach for examining emotional expressions through discourse analysis and ethnographic methods. Drawing on trends in the current literature in science education, we briefly explain the importance of emotions in science education and examine the current research methodologies used in interactional emotion studies. We put forth and substantiate a methodological approach that attends to the interactional, contextual, intertextual, and consequential aspects of emotional expressions. By examining emotional expressions in the discourse in which they are constructed, emotional expressions are identified through semantics, contextualization, and linguistic features. These features make salient four dimensions of emotional expressions: aboutness, frequency, type, and ownership. Drawing on data from a large empirical study of pre-service elementary teachers' emotional expressions about climate change in a science course, we provide illustrative examples to describe what counts as emotional expressions in situ. In doing so we explain how our approach makes salient the nuanced nature of such expressions as well as the broader discourse in which they are constructed and the implications for researching emotional expressions in science education discourse. We suggest reasons why this discourse orientated research methodology can contribute to the interactional study of emotions in science education contexts.

Positive expression of LSD1 and negative expression of E-cadherin correlate with metastasis and poor prognosis of colon cancer.

Science.gov (United States)

Jie, Ding; Zhongmin, Zhang; Guoqing, Liao; Sheng, Liu; Yi, Zhang; Jing, Wen; Liang, Zeng

2013-06-01

The first identified lysine-specific demethylase, LSD1, plays an important role in the metastatic progression of several types of cancer. The aim of this study was to investigate LSD1, E-cadherin, and N-cadherin expression in colon cancer specimens and their clinical significance. The expression of LSD1, E-cadherin, and N-cadherin in colon cancer specimens was determined by immunohistochemistry, and the relationship between the expression of the respective molecules and clinicopathological characteristics was analyzed. The positive expression rates of LSD1, E-cadherin, and N-cadherin in colon cancer specimens were 66.7 % (72/108), 85.2 % (92/108), and 41.7 % (45/108), respectively. LSD1 was significantly more highly expressed in colon cancer specimens classified as high TNM stage lesions and with distant metastasis (P colon cancer specimens classified as high TNM stage lesions and with distant metastasis (P clinical and pathological characteristics (P > 0.05). Correlation analysis revealed that LSD1 expression was negatively correlated with E-cadherin expression (r s = -0.318, P = 0.001), but not evidently correlated with N-cadherin expression (r s = 0.182, P = 0.06). Colon cancer specimens with positive LSD1 expression and negative E-cadherin expression were correlated with significantly lower overall survival. LSD1 showed a significantly higher expression, in contrast to the significantly lower expression of E-cadherin, in colon cancer specimens classified as high TNM stage lesions and with distant metastasis. Positive expression of LSD1 and negative expression of E-cadherin may be predictors of a worse colon cancer prognosis.

Deep-sea ecosystems of the Indian Ocean

Digital Repository Service at National Institute of Oceanography (India)

Ingole, B.S.; Koslow, J.A.

are potential sites for hydrothermal mineralization and contain active vent fields. There are no available estimates for the numbers of seamounts in the Indian Ocean based on echo sounder recordings. Satellite altimetry data indicate that the Indian Ocean has...

[Prosopagnosia and facial expression recognition].

Science.gov (United States)

Koyama, Shinichi

2014-04-01

This paper reviews clinical neuropsychological studies that have indicated that the recognition of a person's identity and the recognition of facial expressions are processed by different cortical and subcortical areas of the brain. The fusiform gyrus, especially the right fusiform gyrus, plays an important role in the recognition of identity. The superior temporal sulcus, amygdala, and medial frontal cortex play important roles in facial-expression recognition. Both facial recognition and facial-expression recognition are highly intellectual processes that involve several regions of the brain.

Facial expressions and pair bonds in hylobatids.

Science.gov (United States)

Florkiewicz, Brittany; Skollar, Gabriella; Reichard, Ulrich H

2018-06-06

Facial expressions are an important component of primate communication that functions to transmit social information and modulate intentions and motivations. Chimpanzees and macaques, for example, produce a variety of facial expressions when communicating with conspecifics. Hylobatids also produce various facial expressions; however, the origin and function of these facial expressions are still largely unclear. It has been suggested that larger facial expression repertoires may have evolved in the context of social complexity, but this link has yet to be tested at a broader empirical basis. The social complexity hypothesis offers a possible explanation for the evolution of complex communicative signals such as facial expressions, because as the complexity of an individual's social environment increases so does the need for communicative signals. We used an intraspecies, pair-focused study design to test the link between facial expressions and sociality within hylobatids, specifically the strength of pair-bonds. The current study compared 206 hr of video and 103 hr of focal animal data for ten hylobatid pairs from three genera (Nomascus, Hoolock, and Hylobates) living at the Gibbon Conservation Center. Using video footage, we explored 5,969 facial expressions along three dimensions: repertoire use, repertoire breadth, and facial expression synchrony [FES]. We then used focal animal data to compare dimensions of facial expressiveness to pair bond strength and behavioral synchrony. Hylobatids in our study overlapped in only half of their facial expressions (50%) with the only other detailed, quantitative study of hylobatid facial expressions, while 27 facial expressions were uniquely observed in our study animals. Taken together, hylobatids have a large facial expression repertoire of at least 80 unique facial expressions. Contrary to our prediction, facial repertoire composition was not significantly correlated with pair bond strength, rates of territorial synchrony

Facial Expression Recognition Through Machine Learning

Directory of Open Access Journals (Sweden)

Nazia Perveen

2015-08-01

Full Text Available Facial expressions communicate non-verbal cues which play an important role in interpersonal relations. Automatic recognition of facial expressions can be an important element of normal human-machine interfaces it might likewise be utilized as a part of behavioral science and in clinical practice. In spite of the fact that people perceive facial expressions for all intents and purposes immediately solid expression recognition by machine is still a challenge. From the point of view of automatic recognition a facial expression can be considered to comprise of disfigurements of the facial parts and their spatial relations or changes in the faces pigmentation. Research into automatic recognition of the facial expressions addresses the issues encompassing the representation and arrangement of static or dynamic qualities of these distortions or face pigmentation. We get results by utilizing the CVIPtools. We have taken train data set of six facial expressions of three persons and for train data set purpose we have total border mask sample 90 and 30 border mask sample for test data set purpose and we use RST- Invariant features and texture features for feature analysis and then classified them by using k- Nearest Neighbor classification algorithm. The maximum accuracy is 90.

Glucose transporters: expression, regulation and cancer

Directory of Open Access Journals (Sweden)

RODOLFO A. MEDINA

2002-01-01

Full Text Available Mammalian cells depend on glucose as a major substrate for energy production. Glucose is transported into the cell via facilitative glucose transporters (GLUT present in all cell types. Many GLUT isoforms have been described and their expression is cell-specific and subject to hormonal and environmental control. The kinetic properties and substrate specificities of the different isoforms are specifically suited to the energy requirements of the particular cell types. Due to the ubiquitousness of these transporters, their differential expression is involved in various disease states such as diabetes, ischemia and cancer. The majority of cancers and isolated cancer cell lines over-express the GLUT family members which are present in the respective tissue of origin under non-cancerous conditions. Moreover, due to the requirement of energy to feed uncontrolled proliferation, cancer cells often express GLUTs which under normal conditions would not be present in these tissues. This over-expression is predominantly associated with the likelihood of metastasis and hence poor patient prognosis. This article presents a review of the current literature on the regulation and expression of GLUT family members and has compiled clinical and research data on GLUT expression in human cancers and in isolated human cancer cell lines.

CNPase Expression in Olfactory Ensheathing Cells

Directory of Open Access Journals (Sweden)

Christine Radtke

2011-01-01

Full Text Available A large body of work supports the proposal that transplantation of olfactory ensheathing cells (OECs into nerve or spinal cord injuries can promote axonal regeneration and remyelination. Yet, some investigators have questioned whether the transplanted OECs associate with axons and form peripheral myelin, or if they recruit endogenous Schwann cells that form myelin. Olfactory bulbs from transgenic mice expressing the enhanced green fluorescent protein (eGFP under the control of the 2-3-cyclic nucleotide 3-phosphodiesterase (CNPase promoter were studied. CNPase is expressed in myelin-forming cells throughout their lineage. We examined CNPase expression in both in situ in the olfactory bulb and in vitro to determine if OECs express CNPase commensurate with their myelination potential. eGFP was observed in the outer nerve layer of the olfactory bulb. Dissociated OECs maintained in culture had both intense eGFP expression and CNPase immunostaining. Transplantation of OECs into transected peripheral nerve longitudinally associated with the regenerated axons. These data indicate that OECs in the outer nerve layer of the olfactory bulb of CNPase transgenic mice express CNPase. Thus, while OECs do not normally form myelin on olfactory nerve axons, their expression of CNPase is commensurate with their potential to form myelin when transplanted into injured peripheral nerve.

Dynamic Facial Expression of Emotion Made Easy

OpenAIRE

Broekens, Joost; Qu, Chao; Brinkman, Willem-Paul

2012-01-01

Facial emotion expression for virtual characters is used in a wide variety of areas. Often, the primary reason to use emotion expression is not to study emotion expression generation per se, but to use emotion expression in an application or research project. What is then needed is an easy to use and flexible, but also validated mechanism to do so. In this report we present such a mechanism. It enables developers to build virtual characters with dynamic affective facial expressions. The mecha...

Multiscale Embedded Gene Co-expression Network Analysis.

Directory of Open Access Journals (Sweden)

Won-Min Song

2015-11-01

Full Text Available Gene co-expression network analysis has been shown effective in identifying functional co-expressed gene modules associated with complex human diseases. However, existing techniques to construct co-expression networks require some critical prior information such as predefined number of clusters, numerical thresholds for defining co-expression/interaction, or do not naturally reproduce the hallmarks of complex systems such as the scale-free degree distribution of small-worldness. Previously, a graph filtering technique called Planar Maximally Filtered Graph (PMFG has been applied to many real-world data sets such as financial stock prices and gene expression to extract meaningful and relevant interactions. However, PMFG is not suitable for large-scale genomic data due to several drawbacks, such as the high computation complexity O(|V|3, the presence of false-positives due to the maximal planarity constraint, and the inadequacy of the clustering framework. Here, we developed a new co-expression network analysis framework called Multiscale Embedded Gene Co-expression Network Analysis (MEGENA by: i introducing quality control of co-expression similarities, ii parallelizing embedded network construction, and iii developing a novel clustering technique to identify multi-scale clustering structures in Planar Filtered Networks (PFNs. We applied MEGENA to a series of simulated data and the gene expression data in breast carcinoma and lung adenocarcinoma from The Cancer Genome Atlas (TCGA. MEGENA showed improved performance over well-established clustering methods and co-expression network construction approaches. MEGENA revealed not only meaningful multi-scale organizations of co-expressed gene clusters but also novel targets in breast carcinoma and lung adenocarcinoma.

Multiscale Embedded Gene Co-expression Network Analysis.

Science.gov (United States)

Song, Won-Min; Zhang, Bin

2015-11-01

Gene co-expression network analysis has been shown effective in identifying functional co-expressed gene modules associated with complex human diseases. However, existing techniques to construct co-expression networks require some critical prior information such as predefined number of clusters, numerical thresholds for defining co-expression/interaction, or do not naturally reproduce the hallmarks of complex systems such as the scale-free degree distribution of small-worldness. Previously, a graph filtering technique called Planar Maximally Filtered Graph (PMFG) has been applied to many real-world data sets such as financial stock prices and gene expression to extract meaningful and relevant interactions. However, PMFG is not suitable for large-scale genomic data due to several drawbacks, such as the high computation complexity O(|V|3), the presence of false-positives due to the maximal planarity constraint, and the inadequacy of the clustering framework. Here, we developed a new co-expression network analysis framework called Multiscale Embedded Gene Co-expression Network Analysis (MEGENA) by: i) introducing quality control of co-expression similarities, ii) parallelizing embedded network construction, and iii) developing a novel clustering technique to identify multi-scale clustering structures in Planar Filtered Networks (PFNs). We applied MEGENA to a series of simulated data and the gene expression data in breast carcinoma and lung adenocarcinoma from The Cancer Genome Atlas (TCGA). MEGENA showed improved performance over well-established clustering methods and co-expression network construction approaches. MEGENA revealed not only meaningful multi-scale organizations of co-expressed gene clusters but also novel targets in breast carcinoma and lung adenocarcinoma.

Marker Protein Expression Combined With Expression Heterogeneity is a Powerful Indicator of Malignancy in Acral Lentiginous Melanomas.

Science.gov (United States)

Cintra Lopes Carapeto, Fernando; Neves Comodo, Andréia; Germano, Andressa; Pereira Guimarães, Daiane; Barcelos, Denise; Fernandes, Mariana; Landman, Gilles

2017-02-01

Samples of acral lentiginous melanomas (ALMs) were obtained from the Department of Pathology at Escola Paulista de Medicina-Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil. Demographic, clinical, and follow-up data were obtained from the charts of Hospital São Paulo. From 2 tissue microarrays containing 60 nevi and quadruplicate samples of ≥1.0-mm of 49 ALM, sections were stained to evaluate SCF, KIT, BRAF, CYCLIND1, MYC, and PTEN immunohistochemical protein expression. Nevi and ALM from 2006 to 2010 were reviewed and collected. All specimens were in the vertical growth phase, and histopathological parameters indicated that tumors were at an advanced stage at diagnosis. Average tumor thickness was 6.95 mm, 63% were ulcerated, average mitotic index was 5 mitotic cells per mm, and 43% were at Clark's level V. Compared with nevi, the χ test showed that ALM significantly correlated with SCF protein expression (P = 0.001) and expression heterogeneity (P < 0.000). Similar findings were observed for KIT (P = 0.005, P = 0.003, respectively), MYC (P < 0.000, P < 0.000), and PTEN (P = 0.005, P < 0.000). Malignancy did not correlate with BRAF and CYCLIN D1 expression (P = 0.053 and P = 0.259, respectively), but it did significantly correlate with their heterogeneous expression (P < 0.000, P = 0.024, respectively). Combined protein expression had an odds ratio of greater malignancy when BRAF and MYC were positive and/or heterogeneously expressed (OR of 78 and 95, respectively). We show that marker protein expression, when combined with heterogeneous expression as shown by immunohistochemistry, is a powerful indicator of malignancy in ALMs, especially, when protein pairs are combined.

Emotion Expression of Robot with Personality

Directory of Open Access Journals (Sweden)

Xue Hu

2013-01-01

Full Text Available A robot emotional expression model based on Hidden Markov Model (HMM is built to enable robots which have different personalities to response in a more satisfactory emotional level. Gross emotion regulation theory and Five Factors Model (FFM which are the theoretical basis are firstly described. And then the importance of the personality effect on the emotion expression process is proposed, and how to make the effect quantization is discussed. After that, the algorithm of HMM is used to describe the process of emotional state transition and expression, and the performance transferring probability affected by personality is calculated. At last, the algorithm model is simulated and applied in a robot platform. The results prove that the emotional expression model can acquire humanlike expressions and improve the human-computer interaction.

Converting nested algebra expressions into flat algebra expressions

NARCIS (Netherlands)

Paredaens, J.; Van Gucht, D.

1992-01-01

Nested relations generalize ordinary flat relations by allowing tuple values to be either atomic or set valued. The nested algebra is a generalization of the flat relational algebra to manipulate nested relations. In this paper we study the expressive power of the nested algebra relative to its

Overexpression of pucC improves the heterologous protein expression level in a Rhodobacter sphaeroides expression system.

Science.gov (United States)

Cheng, L; Chen, G; Ding, G; Zhao, Z; Dong, T; Hu, Z

2015-04-27

The Rhodobacter sphaeroides system has been used to express membrane proteins. However, its low yield has substantially limited its application. In order to promote the protein expression capability of this system, the pucC gene, which plays a crucial role in assembling the R. sphaeroides light-harvesting 2 complex (LH2), was overexpressed. To build a pucC overexpression strain, a pucC overexpression vector was constructed and transformed into R. sphaeroides CQU68. The overexpression efficiency was evaluated by quantitative real-time polymerase chain reaction. A well-used reporter β-glucuronidase (GUS) was fusion-expressed with LH2 to evaluate the heterologous protein expression level. As a result, the cell culture and protein in the pucC overexpression strain showed much higher typical spectral absorption peaks at 800 and 850 nm compared with the non-overexpression strain, suggesting a higher expression level of LH2-GUS fusion protein in the pucC overexpression strain. This result was further confirmed by Western blot, which also showed a much higher level of heterologous protein expression in the pucC overexpression strain. We further compared GUS activity in pucC overexpression and non-overexpression strains, the results of which showed that GUS activity in the pucC overexpression strain was approximately ten-fold that in the non-overexpression strain. These results demonstrate that overexpressed pucC can promote heterologous protein expression levels in R. sphaeroides.

Comet radar explorer

Science.gov (United States)

Farnham, Tony; Asphaug, Erik; Barucci, Antonella; Belton, Mike; Bockelee-Morvan, Dominique; Brownlee, Donald; Capria, Maria Teresa; Carter, Lynn; Chesley, Steve; Farnham, Tony; Gaskell, Robert; Gim, Young; Heggy, Essam; Herique, Alain; Klaasen, Ken; Kofman, Wlodek; Kreslavsky, Misha; Lisse, Casey; Orosei, Roberto; Plaut, Jeff; Scheeres, Dan

The Comet Radar Explorer (CORE) is designed to perform a comprehensive and detailed exploration of the interior, surface, and inner coma structures of a scientifically impor-tant Jupiter family comet. These structures will be used to investigate the origins of cometary nuclei, their physical and geological evolution, and the mechanisms driving their spectacular activity. CORE is a high heritage spacecraft, injected by solar electric propulsion into orbit around a comet. It is capable of coherent deep radar imaging at decameter wavelengths, high resolution stereo color imaging, and near-IR imaging spectroscopy. Its primary objective is to obtain a high-resolution map of the interior structure of a comet nucleus at a resolution of ¿100 elements across the diameter. This structure shall be related to the surface geology and morphology, and to the structural details of the coma proximal to the nucleus. This is an ideal complement to the science from recent comet missions, providing insight into how comets work. Knowing the structure of the interior of a comet-what's inside-and how cometary activity works, is required before we can understand the requirements for a cryogenic sample return mission. But more than that, CORE is fundamental to understanding the origin of comets and their evolution in time. The mission is made feasible at low cost by the use of now-standard MARSIS-SHARAD reflec-tion radar imaging hardware and data processing, together with proven flight heritage of solar electric propulsion. Radar flight heritage has been demonstrated by the MARSIS radar on Mars Express (Picardi et al., Science 2005; Plaut et al., Science 2007), the SHARAD radar onboard the Mars Reconnaissance Orbiter (Seu et al., JGR 2007), and the LRS radar onboard Kaguya (Ono et al, EPS 2007). These instruments have discovered detailed subsurface structure to depths of several kilometers in a variety of terrains on Mars and the Moon. A reflection radar deployed in orbit about a comet

Judgments of subtle facial expressions of emotion.

Science.gov (United States)

Matsumoto, David; Hwang, Hyisung C

2014-04-01

Most studies on judgments of facial expressions of emotion have primarily utilized prototypical, high-intensity expressions. This paper examines judgments of subtle facial expressions of emotion, including not only low-intensity versions of full-face prototypes but also variants of those prototypes. A dynamic paradigm was used in which observers were shown a neutral expression followed by the target expression to judge, and then the neutral expression again, allowing for a simulation of the emergence of the expression from and then return to a baseline. We also examined how signal and intensity clarities of the expressions (explained more fully in the Introduction) were associated with judgment agreement levels. Low-intensity, full-face prototypical expressions of emotion were judged as the intended emotion at rates significantly greater than chance. A number of the proposed variants were also judged as the intended emotions. Both signal and intensity clarities were individually associated with agreement rates; when their interrelationships were taken into account, signal clarity independently predicted agreement rates but intensity clarity did not. The presence or absence of specific muscles appeared to be more important to agreement rates than their intensity levels, with the exception of the intensity of zygomatic major, which was positively correlated with agreement rates for judgments of joy.

Perceiving and producing facial expressions of emotion : The role of dynamic expressions and culture

NARCIS (Netherlands)

Fang, X.

2018-01-01

We spend much of our waking lives interacting with other people, reading their facial expressions to figure out what they might be feeling, thinking, or intending to do next (Ekman 1994; Fridlund 1994). At the same time, we also express our own feelings, thoughts, and intentions through facial

Expression in E. coli systems

DEFF Research Database (Denmark)

Krogsdam, Anne-M; Kristiansen, Karsten; Nøhr, Jane

2003-01-01

intracellularly in soluble form. In E. coli, proteins containing disulfide bonds are best produced by secretion because the disulfide forming foldases reside in the periplasm. Likewise, a correct N-terminus is more likely to be obtained upon secretion. Moreover, potentially toxic proteins are more likely......Owing to cost advantage, speed of production, and often high product yield (up to 50% of total cell protein), expression in Escherichia coli is generally the first choice when attempting to express a recombinant protein. Expression systems exist to produce recombinant protein intracellularly...

Regional Brain Responses Are Biased Toward Infant Facial Expressions Compared to Adult Facial Expressions in Nulliparous Women.

Science.gov (United States)

Li, Bingbing; Cheng, Gang; Zhang, Dajun; Wei, Dongtao; Qiao, Lei; Wang, Xiangpeng; Che, Xianwei

2016-01-01

Recent neuroimaging studies suggest that neutral infant faces compared to neutral adult faces elicit greater activity in brain areas associated with face processing, attention, empathic response, reward, and movement. However, whether infant facial expressions evoke larger brain responses than adult facial expressions remains unclear. Here, we performed event-related functional magnetic resonance imaging in nulliparous women while they were presented with images of matched unfamiliar infant and adult facial expressions (happy, neutral, and uncomfortable/sad) in a pseudo-randomized order. We found that the bilateral fusiform and right lingual gyrus were overall more activated during the presentation of infant facial expressions compared to adult facial expressions. Uncomfortable infant faces compared to sad adult faces evoked greater activation in the bilateral fusiform gyrus, precentral gyrus, postcentral gyrus, posterior cingulate cortex-thalamus, and precuneus. Neutral infant faces activated larger brain responses in the left fusiform gyrus compared to neutral adult faces. Happy infant faces compared to happy adult faces elicited larger responses in areas of the brain associated with emotion and reward processing using a more liberal threshold of p facial expressions compared to adult facial expressions among nulliparous women, and this bias may be modulated by individual differences in Interest-In-Infants and perspective taking ability.

Decreased expression of Beclin 1 correlates closely with Bcl-xL expression and poor prognosis of ovarian carcinoma.

Directory of Open Access Journals (Sweden)

Huan-Xin Lin

Full Text Available It has been suggested that autophagy-related Beclin 1 plays a critical role in the regulation of tumor development and/or progression, but its prognostic significance and relationship with Bcl-xL expression in ovarian carcinoma are unclear.In the present study, the methods of Western blotting and immunohistochemistry (IHC were utilized to investigate the expression status of Beclin 1 and Bcl-xL in fresh ovarian tissues and paraffin-embedded epithelial ovarian tumor tissues. Decreased expression of Beclin 1 was examined by IHC in 8.3% of normal ovaries, in 15.4% of cystadenomas, in 20.0% of borderline tumors, and in 55.6% of ovarian carcinomas, respectively. In ovarian carcinomas, decreased expression of Beclin 1 was correlated closely with ascending histological grade, later pT/pN/pM status and/or advanced clinical stage (P<0.05. In univariate survival analysis, a highly significant association between low-expressed Beclin 1 and shortened patient survival was evaluated in ovarian carcinoma patients (P<0.01, and Beclin 1 expression was an independent prognostic factor as evidenced by multivariate analysis (P = 0.013. In addition, decreased expression of Beclin 1 was inversely correlated with altered expression of Bcl-xL in ovarian carcinoma cohort, and combined analysis further showed that the low Beclin 1/high Bcl-xL group had the lowest survival rate.Our findings suggest that Beclin 1 expression, as examined by IHC, could be served as an additional tool in identifying ovarian carcinoma patients at risk of tumor progression, and predicting patient survival in ovarian carcinomas with increased expression of Bcl-xL.

In Silico Expression Analysis.

Science.gov (United States)

Bolívar, Julio; Hehl, Reinhard; Bülow, Lorenz

2016-01-01

Information on the specificity of cis-sequences enables the design of functional synthetic plant promoters that are responsive to specific stresses. Potential cis-sequences may be experimentally tested, however, correlation of genomic sequence with gene expression data enables an in silico expression analysis approach to bioinformatically assess the stress specificity of candidate cis-sequences prior to experimental verification. The present chapter demonstrates an example for the in silico validation of a potential cis-regulatory sequence responsive to cold stress. The described online tool can be applied for the bioinformatic assessment of cis-sequences responsive to most abiotic and biotic stresses of plants. Furthermore, a method is presented based on a reverted in silico expression analysis approach that predicts highly specific potentially functional cis-regulatory elements for a given stress.

THE USE OF ELECTRONIC DATA PROCESSING IN CORRECTIONS AND LAW ENFORCEMENT,

Science.gov (United States)

Reviews the reasons, methods, accomplishments and goals of the use of electronic data processing in the fields of correction and law enforcement . Suggest...statistical and case history data in building a sounder theoretical base in the field of law enforcement . (Author)

A review of applications of satellite SAR, optical, altimetry and DEM data for surface water modelling, mapping and parameter estimation

NARCIS (Netherlands)

Musa, Z.N.; Popescu, I; Mynett, A.E.

2015-01-01

Hydrological data collection requires deployment of physical infrastructure like rain gauges, water level gauges, as well as use of expensive equipment like echo sounders. Many countries around the world have recorded a decrease in deployment of physical infrastructure for hydrological

Reliability Generalization of the Alcohol Use Disorder Identification Test.

Science.gov (United States)

Shields, Alan L.; Caruso, John C.

2002-01-01

Evaluated the reliability of scores from the Alcohol Use Disorders Identification Test (AUDIT; J. Sounders and others, 1993) in a reliability generalization study based on 17 empirical journal articles. Results show AUDIT scores to be generally reliable for basic assessment. (SLD)

Expression of CD133 in acute leukemia.

Science.gov (United States)

Tolba, Fetnat M; Foda, Mona E; Kamal, Howyda M; Elshabrawy, Deena A

2013-06-01

There have been conflicting results regarding a correlation between CD133 expression and disease outcome. To assess CD133 expression in patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) and to evaluate its correlation with the different clinical and laboratory data as well as its relation to disease outcome, the present study included 60 newly diagnosed acute leukemic patients; 30 ALL patients with a male to female ratio of 1.5:1 and their ages ranged from 9 months to 48 years, and 30 AML patients with a male to female ratio of 1:1 and their ages ranged from 17 to 66 years. Flow cytometric assessment of CD133 expression was performed on blast cells. In ALL, no correlations were elicited between CD133 expression and some monoclonal antibodies, but in AML group, there was a significant positive correlation between CD133 and HLA-DR, CD3, CD7 and TDT, CD13 and CD34. In ALL group, patients with negative CD133 expression achieved complete remission more than patients with positive CD133 expression. In AML group, there was no statistically significant association found between positive CD133 expression and treatment outcome. The Kaplan-Meier curve illustrated a high significant negative correlation between CD133 expression and the overall survival of the AML patients. CD133 expression is an independent prognostic factor in acute leukemia, especially ALL patients and its expression could characterize a group of acute leukemic patients with higher resistance to standard chemotherapy and relapse. CD133 expression was highly associated with poor prognosis in acute leukemic patients.

Synthetic promoter libraries- tuning of gene expression

DEFF Research Database (Denmark)

Hammer, Karin; Mijakovic, Ivan; Jensen, Peter Ruhdal

2006-01-01

knockout and strong overexpression. However, applications such as metabolic optimization and control analysis necessitate a continuous set of expression levels with only slight increments in strength to cover a specific window around the wildtype expression level of the studied gene; this requirement can......The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene...

Modulation of gene expression made easy

DEFF Research Database (Denmark)

Solem, Christian; Jensen, Peter Ruhdal

2002-01-01

A new approach for modulating gene expression, based on randomization of promoter (spacer) sequences, was developed. The method was applied to chromosomal genes in Lactococcus lactis and shown to generate libraries of clones with broad ranges of expression levels of target genes. In one example...... that the method can be applied to modulating the expression of native genes on the chromosome. We constructed a series of strains in which the expression of the las operon, containing the genes pfk, pyk, and ldh, was modulated by integrating a truncated copy of the pfk gene. Importantly, the modulation affected...

Large scale gene expression meta-analysis reveals tissue-specific, sex-biased gene expression in humans

Directory of Open Access Journals (Sweden)

Benjamin Mayne

2016-10-01

Full Text Available The severity and prevalence of many diseases are known to differ between the sexes. Organ specific sex-biased gene expression may underpin these and other sexually dimorphic traits. To further our understanding of sex differences in transcriptional regulation, we performed meta-analyses of sex biased gene expression in multiple human tissues. We analysed 22 publicly available human gene expression microarray data sets including over 2500 samples from 15 different tissues and 9 different organs. Briefly, by using an inverse-variance method we determined the effect size difference of gene expression between males and females. We found the greatest sex differences in gene expression in the brain, specifically in the anterior cingulate cortex, (1818 genes, followed by the heart (375 genes, kidney (224 genes, colon (218 genes and thyroid (163 genes. More interestingly, we found different parts of the brain with varying numbers and identity of sex-biased genes, indicating that specific cortical regions may influence sexually dimorphic traits. The majority of sex-biased genes in other tissues such as the bladder, liver, lungs and pancreas were on the sex chromosomes or involved in sex hormone production. On average in each tissue, 32% of autosomal genes that were expressed in a sex-biased fashion contained androgen or estrogen hormone response elements. Interestingly, across all tissues, we found approximately two-thirds of autosomal genes that were sex-biased were not under direct influence of sex hormones. To our knowledge this is the largest analysis of sex-biased gene expression in human tissues to date. We identified many sex-biased genes that were not under the direct influence of sex chromosome genes or sex hormones. These may provide targets for future development of sex-specific treatments for diseases.

Expressive map design: OGC SLD/SE++ extension for expressive map styles

Science.gov (United States)

Christophe, Sidonie; Duménieu, Bertrand; Masse, Antoine; Hoarau, Charlotte; Ory, Jérémie; Brédif, Mathieu; Lecordix, François; Mellado, Nicolas; Turbet, Jérémie; Loi, Hugo; Hurtut, Thomas; Vanderhaeghe, David; Vergne, Romain; Thollot, Joëlle

2018-05-01

In the context of custom map design, handling more artistic and expressive tools has been identified as a carto-graphic need, in order to design stylized and expressive maps. Based on previous works on style formalization, an approach for specifying the map style has been proposed and experimented for particular use cases. A first step deals with the analysis of inspiration sources, in order to extract `what does make the style of the source', i.e. the salient visual characteristics to be automatically reproduced (textures, spatial arrangements, linear stylization, etc.). In a second step, in order to mimic and generate those visual characteristics, existing and innovative rendering techniques have been implemented in our GIS engine, thus extending the capabilities to generate expressive renderings. Therefore, an extension of the existing cartographic pipeline has been proposed based on the following aspects: 1- extension of the symbolization specifications OGC SLD/SE in order to provide a formalism to specify and reference expressive rendering methods; 2- separate the specification of each rendering method and its parameterization, as metadata. The main contribution has been described in (Christophe et al. 2016). In this paper, we focus firstly on the extension of the cartographic pipeline (SLD++ and metadata) and secondly on map design capabilities which have been experimented on various topographic styles: old cartographic styles (Cassini), artistic styles (watercolor, impressionism, Japanese print), hybrid topographic styles (ortho-imagery & vector data) and finally abstract and photo-realist styles for the geovisualization of costal area. The genericity and interoperability of our approach are promising and have already been tested for 3D visualization.

Intrahepatic expression of interferon alpha & interferon alpha ...

African Journals Online (AJOL)

kemrilib

IFN-α and IFN-α Receptor mRNA expression in the liver. All the patients showed IFN-α gene expression except one patient who had the highest degree of fibrosis (fibrosis grade 5) and HAI Index of 9. IFN- α Receptor mRNA was expressed in 30% (9/30). (Figure 4). Non of the patients with HCC had. IFNα-Rc expression and ...

Renal Gene Expression Database (RGED): a relational database of gene expression profiles in kidney disease.

Science.gov (United States)

Zhang, Qingzhou; Yang, Bo; Chen, Xujiao; Xu, Jing; Mei, Changlin; Mao, Zhiguo

2014-01-01

We present a bioinformatics database named Renal Gene Expression Database (RGED), which contains comprehensive gene expression data sets from renal disease research. The web-based interface of RGED allows users to query the gene expression profiles in various kidney-related samples, including renal cell lines, human kidney tissues and murine model kidneys. Researchers can explore certain gene profiles, the relationships between genes of interests and identify biomarkers or even drug targets in kidney diseases. The aim of this work is to provide a user-friendly utility for the renal disease research community to query expression profiles of genes of their own interest without the requirement of advanced computational skills. Website is implemented in PHP, R, MySQL and Nginx and freely available from http://rged.wall-eva.net. http://rged.wall-eva.net. © The Author(s) 2014. Published by Oxford University Press.

Renal Gene Expression Database (RGED): a relational database of gene expression profiles in kidney disease

Science.gov (United States)

Zhang, Qingzhou; Yang, Bo; Chen, Xujiao; Xu, Jing; Mei, Changlin; Mao, Zhiguo

2014-01-01

We present a bioinformatics database named Renal Gene Expression Database (RGED), which contains comprehensive gene expression data sets from renal disease research. The web-based interface of RGED allows users to query the gene expression profiles in various kidney-related samples, including renal cell lines, human kidney tissues and murine model kidneys. Researchers can explore certain gene profiles, the relationships between genes of interests and identify biomarkers or even drug targets in kidney diseases. The aim of this work is to provide a user-friendly utility for the renal disease research community to query expression profiles of genes of their own interest without the requirement of advanced computational skills. Availability and implementation: Website is implemented in PHP, R, MySQL and Nginx and freely available from http://rged.wall-eva.net. Database URL: http://rged.wall-eva.net PMID:25252782

Gene expression and gene therapy imaging

International Nuclear Information System (INIS)

Rome, Claire; Couillaud, Franck; Moonen, Chrit T.W.

2007-01-01

The fast growing field of molecular imaging has achieved major advances in imaging gene expression, an important element of gene therapy. Gene expression imaging is based on specific probes or contrast agents that allow either direct or indirect spatio-temporal evaluation of gene expression. Direct evaluation is possible with, for example, contrast agents that bind directly to a specific target (e.g., receptor). Indirect evaluation may be achieved by using specific substrate probes for a target enzyme. The use of marker genes, also called reporter genes, is an essential element of MI approaches for gene expression in gene therapy. The marker gene may not have a therapeutic role itself, but by coupling the marker gene to a therapeutic gene, expression of the marker gene reports on the expression of the therapeutic gene. Nuclear medicine and optical approaches are highly sensitive (detection of probes in the picomolar range), whereas MRI and ultrasound imaging are less sensitive and require amplification techniques and/or accumulation of contrast agents in enlarged contrast particles. Recently developed MI techniques are particularly relevant for gene therapy. Amongst these are the possibility to track gene therapy vectors such as stem cells, and the techniques that allow spatiotemporal control of gene expression by non-invasive heating (with MRI guided focused ultrasound) and the use of temperature sensitive promoters. (orig.)

Behavioral experience induces zif268 expression in mature granule cells but suppresses its expression in immature granule cells

Science.gov (United States)

Huckleberry, Kylie A.; Kane, Gary A.; Mathis, Rita J.; Cook, Sarah G.; Clutton, Jonathan E.; Drew, Michael R.

2015-01-01

Thousands of neurons are born each day in the dentate gyrus (DG), but many of these cells die before reaching maturity. Both death and survival of adult-born neurons are regulated by neuronal activity in the DG. The immediate-early gene (IEG) zif268 appears to be an important mediator of these effects, as its expression can be induced by neural activity and knockout of zif268 impairs survival of adult-born neurons (Richardson et al., 1992; Veyrac et al., 2013). Despite the apparent importance of zif268 for adult neurogenesis, its behavior-induced expression has not been fully characterized in adult-born neurons. Here we characterize behavior-evoked expression of zif268 in mature and newborn dentate granule cells (DGCs). We first quantified zif268 expression in doublecortin-positive (DCX+) immature neurons and in the general granule cell population after brief exposure to a novel environment (NE). In the general granule cell population, zif268 expression peaked 1 h after NE exposure and returned to baseline by 8 h post-exposure. However, in the DCX+ cells, zif268 expression was suppressed relative to home cage for at least 8 h post-exposure. We next asked whether suppression of zif268 in DCX+ immature cells occurs in other behavioral paradigms that recruit the hippocampus. Exposure to Morris water maze (MWM) training, an enriched environment, or a NE caused approximately equal suppression of zif268 expression in DCX+ cells and approximately equal activation of zif268 expression among the general granule cell population. The same behavioral procedures activated zif268 expression in 6-week-old BrdU-labeled adult-born neurons, indicating that zif268 suppression is specific to immature neurons. Finally, we asked whether zif268 suppression varied as a function of age within the DCX+ population, which ranges in age from 0 to approximately 4 weeks. NE exposure had no significant effect on zif268 expression in 2- or 4-week-old BrdU-labeled neurons, but it significantly

Definition, Detection and Generation of Iyashi Expressions

Science.gov (United States)

Kitaoka, Tetsuko; Diago, Luis A.; Hagiwara, Ichiro; Kitazaki, Satoshi; Yamane, Shigeru

This paper concerns the engineering analysis of “Iyashi”, a peculiar concept to the Japanese, which affect person's heart and may change their expression and behavior. We have integrated the advocator's view of “Iyashi”, analyzed the social background of “Iyashi” and have defined Iyashi and also the Iyashi expression. As the facial expression is the special and important stimulus for both observers and people who show expressions, we want to prove the existence of expressions that change the observer's emotion with Iyashi. We have developed the system to clarify the combination of facial features important for Iyashi through the psychological experiments and the analysis by Holographic Neural Networks (HNN). HNN analysis gave the structure of the Iyashi expression, that is the important combination of the physical facial parameters contributing to the high degree of Iyashi. Based on the structure of Iyashi we are able to generate the Iyashi expression appropriate for each person.

Adaptive Evolution of Gene Expression in Drosophila.

Science.gov (United States)

Nourmohammad, Armita; Rambeau, Joachim; Held, Torsten; Kovacova, Viera; Berg, Johannes; Lässig, Michael

2017-08-08

Gene expression levels are important quantitative traits that link genotypes to molecular functions and fitness. In Drosophila, population-genetic studies have revealed substantial adaptive evolution at the genomic level, but the evolutionary modes of gene expression remain controversial. Here, we present evidence that adaptation dominates the evolution of gene expression levels in flies. We show that 64% of the observed expression divergence across seven Drosophila species are adaptive changes driven by directional selection. Our results are derived from time-resolved data of gene expression divergence across a family of related species, using a probabilistic inference method for gene-specific selection. Adaptive gene expression is stronger in specific functional classes, including regulation, sensory perception, sexual behavior, and morphology. Moreover, we identify a large group of genes with sex-specific adaptation of expression, which predominantly occurs in males. Our analysis opens an avenue to map system-wide selection on molecular quantitative traits independently of their genetic basis. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Adaptive Evolution of Gene Expression in Drosophila

Directory of Open Access Journals (Sweden)

Armita Nourmohammad

2017-08-01

Full Text Available Gene expression levels are important quantitative traits that link genotypes to molecular functions and fitness. In Drosophila, population-genetic studies have revealed substantial adaptive evolution at the genomic level, but the evolutionary modes of gene expression remain controversial. Here, we present evidence that adaptation dominates the evolution of gene expression levels in flies. We show that 64% of the observed expression divergence across seven Drosophila species are adaptive changes driven by directional selection. Our results are derived from time-resolved data of gene expression divergence across a family of related species, using a probabilistic inference method for gene-specific selection. Adaptive gene expression is stronger in specific functional classes, including regulation, sensory perception, sexual behavior, and morphology. Moreover, we identify a large group of genes with sex-specific adaptation of expression, which predominantly occurs in males. Our analysis opens an avenue to map system-wide selection on molecular quantitative traits independently of their genetic basis.

Correlation of Slug gene expression with lymph node metastasis and invasion molecule expression in oral squamous cell carcinoma tissue

Directory of Open Access Journals (Sweden)

Shan-Ming Lu

2017-10-01

Full Text Available Objective: To study the correlation of Slug gene expression with lymph node metastasis and invasion molecule expression in oral squamous cell carcinoma tissue. Methods: Oral squamous cell carcinoma tissue surgical removed in Affiliated Stomatological Hospital of Nanjing Medical University between March 2015 and April 2017 was selected and divided into the oral squamous cell carcinoma tissue with neck lymph node metastasis and the oral squamous cell carcinoma tissues without lymph node metastasis according to the condition of lymph node metastasis. The expression of Slug, epithelial-mesenchymal transition molecules and invasion molecules in the oral squamous cell carcinoma tissue were detected. Results: Slug, N-cadherin, Vimentin, CD147, OPN, GRP78, SDF-1 and CXCR4 protein expression in oral squamous cell carcinoma tissue with neck lymph node metastasis were significantly higher than those in oral squamous cell carcinoma tissue without lymph node metastasis while E-cadherin, P120ctn and ZO-1 protein expression were significantly lower than those in oral squamous cell carcinoma tissue without lymph node metastasis; N-cadherin, Vimentin, CD147, OPN, GRP78, SDF-1 and CXCR4 protein expression in oral squamous cell carcinoma tissue with high Slug expression were significantly higher than those in oral squamous cell carcinoma tissue with low Slug expression while E-cadherin, P120ctn and ZO-1 protein expression were significantly lower than those in oral squamous cell carcinoma tissue with low Slug expression. Conclusion: The highly expressed Slug in oral squamous cell carcinoma tissue can promote the epithelial-mesenchymal transition and invasion of the cells to participate in the lymph node metastasis of tumor cells.

Social Use of Facial Expressions in Hylobatids

Science.gov (United States)

Scheider, Linda; Waller, Bridget M.; Oña, Leonardo; Burrows, Anne M.; Liebal, Katja

2016-01-01

Non-human primates use various communicative means in interactions with others. While primate gestures are commonly considered to be intentionally and flexibly used signals, facial expressions are often referred to as inflexible, automatic expressions of affective internal states. To explore whether and how non-human primates use facial expressions in specific communicative interactions, we studied five species of small apes (gibbons) by employing a newly established Facial Action Coding System for hylobatid species (GibbonFACS). We found that, despite individuals often being in close proximity to each other, in social (as opposed to non-social contexts) the duration of facial expressions was significantly longer when gibbons were facing another individual compared to non-facing situations. Social contexts included grooming, agonistic interactions and play, whereas non-social contexts included resting and self-grooming. Additionally, gibbons used facial expressions while facing another individual more often in social contexts than non-social contexts where facial expressions were produced regardless of the attentional state of the partner. Also, facial expressions were more likely ‘responded to’ by the partner’s facial expressions when facing another individual than non-facing. Taken together, our results indicate that gibbons use their facial expressions differentially depending on the social context and are able to use them in a directed way in communicative interactions with other conspecifics. PMID:26978660

TRPA1 expression levels and excitability brake by KV channels influence cold sensitivity of TRPA1-expressing neurons.

Science.gov (United States)

Memon, Tosifa; Chase, Kevin; Leavitt, Lee S; Olivera, Baldomero M; Teichert, Russell W

2017-06-14

The molecular sensor of innocuous (painless) cold sensation is well-established to be transient receptor potential cation channel, subfamily M, member 8 (TRPM8). However, the role of transient receptor potential cation channel, subfamily A, member 1 (TRPA1) in noxious (painful) cold sensation has been controversial. We find that TRPA1 channels contribute to the noxious cold sensitivity of mouse somatosensory neurons, independent of TRPM8 channels, and that TRPA1-expressing neurons are largely non-overlapping with TRPM8-expressing neurons in mouse dorsal-root ganglia (DRG). However, relatively few TRPA1-expressing neurons (e.g., responsive to allyl isothiocyanate or AITC, a selective TRPA1 agonist) respond overtly to cold temperature in vitro, unlike TRPM8-expressing neurons, which almost all respond to cold. Using somatosensory neurons from TRPM8-/- mice and subtype-selective blockers of TRPM8 and TRPA1 channels, we demonstrate that responses to cold temperatures from TRPA1-expressing neurons are mediated by TRPA1 channels. We also identify two factors that affect the cold-sensitivity of TRPA1-expressing neurons: (1) cold-sensitive AITC-sensitive neurons express relatively more TRPA1 transcripts than cold-insensitive AITC-sensitive neurons and (2) voltage-gated potassium (K V ) channels attenuate the cold-sensitivity of some TRPA1-expressing neurons. The combination of these two factors, combined with the relatively weak agonist-like activity of cold temperature on TRPA1 channels, partially explains why few TRPA1-expressing neurons respond to cold. Blocking K V channels also reveals another subclass of noxious cold-sensitive DRG neurons that do not express TRPM8 or TRPA1 channels. Altogether, the results of this study provide novel insights into the cold-sensitivity of different subclasses of somatosensory neurons. Copyright © 2017 IBRO. Published by Elsevier Ltd. All rights reserved.

The motivation to express prejudice

Science.gov (United States)

Forscher, Patrick S.; Cox, William T. L.; Graetz, Nicholas; Devine, Patricia G.

2015-01-01

Contemporary prejudice research focuses primarily on people who are motivated to respond without prejudice and the ways in which unintentional bias can cause these people to act inconsistent with this motivation. However, some real-world phenomena (e.g., hate speech, hate crimes) and experimental findings (e.g., Plant & Devine, 2001; 2009) suggest that some expressions of prejudice are intentional. These phenomena and findings are difficult to explain solely from the motivations to respond without prejudice. We argue that some people are motivated to express prejudice, and we develop the motivation to express prejudice (MP) scale to measure this motivation. In seven studies involving more than 6,000 participants, we demonstrate that, across scale versions targeted at Black people and gay men, the MP scale has good reliability and convergent, discriminant, and predictive validity. In normative climates that prohibit prejudice, the internal and external motivations to express prejudice are functionally non-independent, but they become more independent when normative climates permit more prejudice toward a target group. People high in the motivation to express prejudice are relatively likely to resist pressure to support programs promoting intergroup contact and vote for political candidates who support oppressive policies. The motivation to express prejudice predicted these outcomes even when controlling for attitudes and the motivations to respond without prejudice. This work encourages contemporary prejudice researchers to broaden the range of samples, target groups, and phenomena that they study, and more generally to consider the intentional aspects of negative intergroup behavior. PMID:26479365

Differential expression of homeobox-containing genes Msx-1 and Msx-2 and homeoprotein Msx-2 expression during chick craniofacial development.

Science.gov (United States)

Nishikawa, K; Nakanishi, T; Aoki, C; Hattori, T; Takahashi, K; Taniguchi, S

1994-03-01

The expression pattern of chick Msx-1 and Msx-2 homeobox genes in craniofacial primordia was examined by in situ hybridization using cRNA probes. Both genes were expressed in the distal region of the facial primordia, where the distribution of Msx-2 expression was restricted distally within the Msx-1 expression domain. On the contrary, Msx-2 expression in the lateral choroid plexus and cranial skull was broader and more intensive than Msx-1 expression. Our findings suggest that these two genes cooperate to play differential roles in craniofacial development. Msx-2 protein was detected immunohistochemically, and its localization essentially corresponded to the mRNA expression pattern, substantiating the involvement of Msx-2 protein as a transcriptional regulator in developing limb and face.

DEXTER: Disease-Expression Relation Extraction from Text.

Science.gov (United States)

Gupta, Samir; Dingerdissen, Hayley; Ross, Karen E; Hu, Yu; Wu, Cathy H; Mazumder, Raja; Vijay-Shanker, K

2018-01-01

Gene expression levels affect biological processes and play a key role in many diseases. Characterizing expression profiles is useful for clinical research, and diagnostics and prognostics of diseases. There are currently several high-quality databases that capture gene expression information, obtained mostly from large-scale studies, such as microarray and next-generation sequencing technologies, in the context of disease. The scientific literature is another rich source of information on gene expression-disease relationships that not only have been captured from large-scale studies but have also been observed in thousands of small-scale studies. Expression information obtained from literature through manual curation can extend expression databases. While many of the existing databases include information from literature, they are limited by the time-consuming nature of manual curation and have difficulty keeping up with the explosion of publications in the biomedical field. In this work, we describe an automated text-mining tool, Disease-Expression Relation Extraction from Text (DEXTER) to extract information from literature on gene and microRNA expression in the context of disease. One of the motivations in developing DEXTER was to extend the BioXpress database, a cancer-focused gene expression database that includes data derived from large-scale experiments and manual curation of publications. The literature-based portion of BioXpress lags behind significantly compared to expression information obtained from large-scale studies and can benefit from our text-mined results. We have conducted two different evaluations to measure the accuracy of our text-mining tool and achieved average F-scores of 88.51 and 81.81% for the two evaluations, respectively. Also, to demonstrate the ability to extract rich expression information in different disease-related scenarios, we used DEXTER to extract information on differential expression information for 2024 genes in lung

TRANSGENIC PLANTS EXPRESSING BACILLUS THURINGIENSIS DELTA-ENDOTOXINS

Institute of Scientific and Technical Information of China (English)

Hua-rong,Li; BrendaOppert; KunYanZhu; RandallA.Higgins; Fang-nengHuang; LawrentL.Buschman

2003-01-01

Commercial varieties of transgenic Bacillus thuringiensis (Bt) plants have been developed in many countries to control target pests. Initially, the expression of native Bt genes in plants was low due to mRNA instability, improper splicing, and post-translation modifications. Subsequently, modifications of the native Bt genes greatly enhanced expression levels. This is a review of the developments that made modem high-expression transgenic Bt plants possible, with an emphasis on the reasons for the low-level expression of native Bt genes in plant systems, and the techniques that have been used to improve plant expression of Bt toxin genes.

VEGF expression in hepatectomized tumor-bearing mice.

Science.gov (United States)

Andrini, L; Blanco, A Fernandez; Inda, A; García, M; Garcia, A; Errecalde, A

2011-01-01

The experiments were designed in order to study the VEGF expression in intact (group I), hepatectomized (group II), and hepatectomized-tumor bearing mice (group III) throughout one complete circadian time span. Adult male mice were used for the VEGF expression study. The statistical analysis was performed using analysis of variance (ANOVA). The results showed statistical differences in the VEGF expression between groups I and II, but the most significant differences were found between groups I and III. In conclusion, these expressions have a circadian rhythm in all groups; moreover, in group III, this expression was higher and appeared before than in the others.

C-reactive protein inhibits survivin expression via Akt/mTOR pathway downregulation by PTEN expression in cardiac myocytes.

Directory of Open Access Journals (Sweden)

Beom Seob Lee

Full Text Available C-reactive protein (CRP is one of the most important biomarkers for arteriosclerosis and cardiovascular disease. Recent studies have shown that CRP affects cell cycle and inflammatory process in cardiac myocytes. Survivin is also involved in cardiac myocytes replication and apoptosis. Reduction of survivin expression is associated with less favorable cardiac remodeling in animal models. However, the effect of CRP on survivin expression and its cellular mechanism has not yet been studied. We demonstrated that treatment of CRP resulted in a significant decrease of survivin protein expression in a concentration-dependent manner in cardiac myocytes. The upstream signaling proteins of survivin, such as Akt, mTOR and p70S6K, were also downregulated by CRP treatment. In addition, CRP increased the protein and mRNA levels of PTEN. The siRNA transfection or specific inhibitor treatment for PTEN restored the CRP-induced downregulation of Akt/mTOR/p70S6K pathway and survivin protein expression. Moreover, pretreatment with a specific p53 inhibitor decreased the CRP-induced PTEN expression. ERK-specific inhibitor also blocked the p53 phosphorylation and PTEN expression induced by CRP. Our study provides a novel insight into CRP-induced downregulation of survivin protein expression in cardiac myocytes through mechanisms that involved in downregulation of Akt/mTOR/p70S6K pathway by expression of PTEN.

During development intense Sox2 expression marks not only Prox1-expressing taste bud cell but also perigemmal cell lineages.

Science.gov (United States)

Nakayama, Ayumi; Miura, Hirohito; Ooki, Makoto; Harada, Shuitsu

2015-03-01

Sox2 is proposed to regulate the differentiation of bipotential progenitor cells into taste bud cells. However, detailed expression of Sox2 remains unclear. In this report, Sox2 expression during taste bud development in the fungiform (FF), circumvallate (CV) and soft palate (SP) areas is examined together with Prox1. First, we immunohistochemically checked Prox1 expression in adults and found that almost all taste bud cells are Prox1-positive. During FF development, intense Sox2 expression was restricted to taste bud primordia expressing Prox1 at E12.5. However, at E14.5, Sox2 was intensely expressed outside the developing taste buds resolving to perigemmal Sox2 expression in adults. In the SP, at E14.5, taste bud primordia emerged as Prox1-expressing cell clusters. However, intense Sox2 expression was not restricted to taste bud primordia but was detected widely in the epithelium. During development, Sox2 expression outside developing taste buds was generally down-regulated but was retained in the perigemmal region similarly to that in the FF. In the CV, the initial stage of taste bud development remained unclear because of the lack of taste bud primordia comparable to that in the FF and SP. Here, we show that Prox1-expressing cells appear in the apical epithelium at E12.5, in the inner trench wall at E17.5 and in the outer trench wall at E18.5. Sox2 was again not restricted to developing taste bud cells expressing Prox1 during CV development. The expression patterns support that Sox2 does not serve as a cell fate selector between taste bud cells and surrounding keratinocytes but rather may contribute to them both.

DAG expression: high-throughput gene expression analysis of real-time PCR data using standard curves for relative quantification.

Directory of Open Access Journals (Sweden)

María Ballester

Full Text Available BACKGROUND: Real-time quantitative PCR (qPCR is still the gold-standard technique for gene-expression quantification. Recent technological advances of this method allow for the high-throughput gene-expression analysis, without the limitations of sample space and reagent used. However, non-commercial and user-friendly software for the management and analysis of these data is not available. RESULTS: The recently developed commercial microarrays allow for the drawing of standard curves of multiple assays using the same n-fold diluted samples. Data Analysis Gene (DAG Expression software has been developed to perform high-throughput gene-expression data analysis using standard curves for relative quantification and one or multiple reference genes for sample normalization. We discuss the application of DAG Expression in the analysis of data from an experiment performed with Fluidigm technology, in which 48 genes and 115 samples were measured. Furthermore, the quality of our analysis was tested and compared with other available methods. CONCLUSIONS: DAG Expression is a freely available software that permits the automated analysis and visualization of high-throughput qPCR. A detailed manual and a demo-experiment are provided within the DAG Expression software at http://www.dagexpression.com/dage.zip.

Observations by the CUTLASS radar, HF Doppler, oblique ionospheric sounding, and TEC from GPS during a magnetic storm

Directory of Open Access Journals (Sweden)

D. V. Blagoveshchensky

2005-07-01

Full Text Available Multi-diagnostic observations, covering a significant area of northwest Europe, were made during the magnetic storm interval (28–29 April 2001 that occurred during the High Rate SolarMax IGS/GPS-campaign. HF radio observations were made with vertical sounders (St. Petersburg and Sodankyla, oblique incidence sounders (OIS, on paths from Murmansk to St. Petersburg, 1050 km, and Inskip to Leicester, 170 km, Doppler sounders, on paths from Cyprus to St. Petersburg, 2800 km, and Murmansk to St. Petersburg, and a coherent scatter radar (CUTLASS, Hankasalmi, Finland. These, together with total electron content (TEC measurements made at GPS stations from the Euref network in northwest Europe, are presented in this paper. A broad comparison of radio propagation data with ionospheric data at high and mid latitudes, under quiet and disturbed conditions, was undertaken. This analysis, together with a geophysical interpretation, allow us to better understand the nature of the ionospheric processes which occur during geomagnetic storms. The peculiarity of the storm was that it comprised of three individual substorms, the first of which appears to have been triggered by a compression of the magnetosphere. Besides the storm effects, we have also studied substorm effects in the observations separately, providing an improved understanding of the storm/substorm relationship. The main results of the investigations are the following. A narrow trough is formed some 10h after the storm onset in the TEC which is most likely a result of enhanced ionospheric convection. An enhancement in TEC some 2–3 h after the storm onset is most likely a result of heating and upwelling of the auroral ionosphere caused by enhanced currents. The so-called main effect on ionospheric propagation was observed at mid-latitudes during the first two substorms, but only during the first substorm at high latitudes. Ionospheric irregularities observed by CUTLASS were clearly related to the

Online handwritten mathematical expression recognition

Science.gov (United States)

Büyükbayrak, Hakan; Yanikoglu, Berrin; Erçil, Aytül

2007-01-01

We describe a system for recognizing online, handwritten mathematical expressions. The system is designed with a user-interface for writing scientific articles, supporting the recognition of basic mathematical expressions as well as integrals, summations, matrices etc. A feed-forward neural network recognizes symbols which are assumed to be single-stroke and a recursive algorithm parses the expression by combining neural network output and the structure of the expression. Preliminary results show that writer-dependent recognition rates are very high (99.8%) while writer-independent symbol recognition rates are lower (75%). The interface associated with the proposed system integrates the built-in recognition capabilities of the Microsoft's Tablet PC API for recognizing textual input and supports conversion of hand-drawn figures into PNG format. This enables the user to enter text, mathematics and draw figures in a single interface. After recognition, all output is combined into one LATEX code and compiled into a PDF file.

Micro-Expression Recognition Using Color Spaces.

Science.gov (United States)

Wang, Su-Jing; Yan, Wen-Jing; Li, Xiaobai; Zhao, Guoying; Zhou, Chun-Guang; Fu, Xiaolan; Yang, Minghao; Tao, Jianhua

2015-12-01

Micro-expressions are brief involuntary facial expressions that reveal genuine emotions and, thus, help detect lies. Because of their many promising applications, they have attracted the attention of researchers from various fields. Recent research reveals that two perceptual color spaces (CIELab and CIELuv) provide useful information for expression recognition. This paper is an extended version of our International Conference on Pattern Recognition paper, in which we propose a novel color space model, tensor independent color space (TICS), to help recognize micro-expressions. In this paper, we further show that CIELab and CIELuv are also helpful in recognizing micro-expressions, and we indicate why these three color spaces achieve better performance. A micro-expression color video clip is treated as a fourth-order tensor, i.e., a four-dimension array. The first two dimensions are the spatial information, the third is the temporal information, and the fourth is the color information. We transform the fourth dimension from RGB into TICS, in which the color components are as independent as possible. The combination of dynamic texture and independent color components achieves a higher accuracy than does that of RGB. In addition, we define a set of regions of interests (ROIs) based on the facial action coding system and calculated the dynamic texture histograms for each ROI. Experiments are conducted on two micro-expression databases, CASME and CASME 2, and the results show that the performances for TICS, CIELab, and CIELuv are better than those for RGB or gray.

The functional landscape of mouse gene expression

Directory of Open Access Journals (Sweden)

Zhang Wen

2004-12-01

Full Text Available Abstract Background Large-scale quantitative analysis of transcriptional co-expression has been used to dissect regulatory networks and to predict the functions of new genes discovered by genome sequencing in model organisms such as yeast. Although the idea that tissue-specific expression is indicative of gene function in mammals is widely accepted, it has not been objectively tested nor compared with the related but distinct strategy of correlating gene co-expression as a means to predict gene function. Results We generated microarray expression data for nearly 40,000 known and predicted mRNAs in 55 mouse tissues, using custom-built oligonucleotide arrays. We show that quantitative transcriptional co-expression is a powerful predictor of gene function. Hundreds of functional categories, as defined by Gene Ontology 'Biological Processes', are associated with characteristic expression patterns across all tissues, including categories that bear no overt relationship to the tissue of origin. In contrast, simple tissue-specific restriction of expression is a poor predictor of which genes are in which functional categories. As an example, the highly conserved mouse gene PWP1 is widely expressed across different tissues but is co-expressed with many RNA-processing genes; we show that the uncharacterized yeast homolog of PWP1 is required for rRNA biogenesis. Conclusions We conclude that 'functional genomics' strategies based on quantitative transcriptional co-expression will be as fruitful in mammals as they have been in simpler organisms, and that transcriptional control of mammalian physiology is more modular than is generally appreciated. Our data and analyses provide a public resource for mammalian functional genomics.

Morphological features in the Bay of Bengal

Digital Repository Service at National Institute of Oceanography (India)

Sarma, K.V.L.N.S.; Ramana, M.V.; Subrahmanyam, V.; Krishna, K.S.; Ramprasad, T.; Desa, M.

history of the Fan. After India's soft collision with the Eurasian plate, these events may have played a critical role in shaping various morphological features since late Eocene in the Bay of Bengal. The present 12 kHz Echo sounder data collected along...

Geostatistical and multivariate modelling for large scale quantitative mapping of seafloor sediments using sparse datasets, a case study from the Cleaverbank area (the Netherlands)

NARCIS (Netherlands)

Alevizos, Evangelos; Siemes, K.; Janmaat, J.; Snellen, M.; Simons, D.G.; Greinert, J

2016-01-01

Quantitative mapping of seafloor sediment properties (eg. grain size) requires the input of comprehensive Multi-Beam Echo Sounder (MBES) datasets along with adequate ground truth for establishing a functional relation between them. MBES surveys in extensive shallow shelf areas can be a rather

Corporate financial restructuring in Asia: implications for financial stability

OpenAIRE

Michael Pomerleano

2007-01-01

Corporate financial fragility preceding the Asian financial crisis heightened vulnerabilities. Many countries in the region undertook significant corporate financial restructuring after the crisis, with some countries bouncing back much faster than others. These sounder corporate financial practices bode well for financial stability.

Sensitivity of tidal sand wave characteristics to environmental parameters: A combined data analysis and modelling approach

NARCIS (Netherlands)

van Santen, R.B.; de Swart, H.E.; van Dijk, T.A.G.P.

2011-01-01

An integrated field data-modelling approach is employed to investigate relationships between the wavelength of tidal sand waves and four environmental parameters: tidal current amplitude, water depth, tidal ellipticity and median grain size. From echo sounder data at 23 locations on the Dutch

MLS/Aura Near-Real-Time L2 Nitrous Oxide (N2O) Mixing Ratio V003

Data.gov (United States)

National Aeronautics and Space Administration — ML2N2O_NRT is the EOS Aura Microwave Limb Sounder (MLS) Near-Real-Time (NRT) product for nitrous oxide (N2O). This product contains daily N2O profiles taken from the...

Profiling Gene Expression in Germinating Brassica Roots.

Science.gov (United States)

Park, Myoung Ryoul; Wang, Yi-Hong; Hasenstein, Karl H

2014-01-01

Based on previously developed solid-phase gene extraction (SPGE) we examined the mRNA profile in primary roots of Brassica rapa seedlings for highly expressed genes like ACT7 (actin7), TUB (tubulin1), UBQ (ubiquitin), and low expressed GLK (glucokinase) during the first day post-germination. The assessment was based on the mRNA load of the SPGE probe of about 2.1 ng. The number of copies of the investigated genes changed spatially along the length of primary roots. The expression level of all genes differed significantly at each sample position. Among the examined genes ACT7 expression was most even along the root. UBQ was highest at the tip and root-shoot junction (RS). TUB and GLK showed a basipetal gradient. The temporal expression of UBQ was highest in the MZ 9 h after primary root emergence and higher than at any other sample position. Expressions of GLK in EZ and RS increased gradually over time. SPGE extraction is the result of oligo-dT and oligo-dA hybridization and the results illustrate that SPGE can be used for gene expression profiling at high spatial and temporal resolution. SPGE needles can be used within two weeks when stored at 4 °C. Our data indicate that gene expression studies that are based on the entire root miss important differences in gene expression that SPGE is able to resolve for example growth adjustments during gravitropism.

Cyclin D1 expression in prostate carcinoma

Energy Technology Data Exchange (ETDEWEB)

Pereira, R.A.; Ravinal, R.C.; Costa, R.S.; Lima, M.S. [Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Patologia, Ribeirão Preto, SP, Brasil, Departamento de Patologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Tucci, S. [Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Urologia, Ribeirão Preto, SP, Brasil, Divisão de Urologia, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Muglia, V.F. [Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Medicina Interna (Centro de Ciência da Imagem), Ribeirão Preto, SP, Brasil, Departamento de Medicina Interna (Centro de Ciência da Imagem), Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Reis, R.B. Dos [Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Urologia, Ribeirão Preto, SP, Brasil, Divisão de Urologia, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Silva, G.E.B. [Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Patologia, Ribeirão Preto, SP, Brasil, Departamento de Patologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil)

2014-05-09

The purpose of this study was to investigate the relationship between cyclin D1 expression and clinicopathological parameters in patients with prostate carcinoma. We assessed cyclin D1 expression by conventional immunohistochemistry in 85 patients who underwent radical prostatectomy for prostate carcinoma and 10 normal prostate tissue samples retrieved from autopsies. We measured nuclear immunostaining in the entire tumor area and based the results on the percentage of positive tumor cells. The preoperative prostate-specific antigen (PSA) level was 8.68±5.16 ng/mL (mean±SD). Cyclin D1 staining was positive (cyclin D1 expression in >5% of tumor cells) in 64 cases (75.4%) and negative (cyclin D1 expression in ≤5% of tumor cells) in 21 cases (including 15 cases with no immunostaining). Normal prostate tissues were negative for cyclin D1. Among patients with a high-grade Gleason score (≥7), 86% of patients demonstrated cyclin D1 immunostaining of >5% (P<0.05). In the crude analysis of cyclin D1 expression, the high-grade Gleason score group showed a mean expression of 39.6%, compared to 26.9% in the low-grade Gleason score group (P<0.05). Perineural invasion tended to be associated with cyclin D1 expression (P=0.07), whereas cyclin D1 expression was not associated with PSA levels or other parameters. Our results suggest that high cyclin D1 expression could be a potential marker for tumor aggressiveness.

Cyclin D1 expression in prostate carcinoma

International Nuclear Information System (INIS)

Pereira, R.A.; Ravinal, R.C.; Costa, R.S.; Lima, M.S.; Tucci, S.; Muglia, V.F.; Reis, R.B. Dos; Silva, G.E.B.

2014-01-01

The purpose of this study was to investigate the relationship between cyclin D1 expression and clinicopathological parameters in patients with prostate carcinoma. We assessed cyclin D1 expression by conventional immunohistochemistry in 85 patients who underwent radical prostatectomy for prostate carcinoma and 10 normal prostate tissue samples retrieved from autopsies. We measured nuclear immunostaining in the entire tumor area and based the results on the percentage of positive tumor cells. The preoperative prostate-specific antigen (PSA) level was 8.68±5.16 ng/mL (mean±SD). Cyclin D1 staining was positive (cyclin D1 expression in >5% of tumor cells) in 64 cases (75.4%) and negative (cyclin D1 expression in ≤5% of tumor cells) in 21 cases (including 15 cases with no immunostaining). Normal prostate tissues were negative for cyclin D1. Among patients with a high-grade Gleason score (≥7), 86% of patients demonstrated cyclin D1 immunostaining of >5% (P<0.05). In the crude analysis of cyclin D1 expression, the high-grade Gleason score group showed a mean expression of 39.6%, compared to 26.9% in the low-grade Gleason score group (P<0.05). Perineural invasion tended to be associated with cyclin D1 expression (P=0.07), whereas cyclin D1 expression was not associated with PSA levels or other parameters. Our results suggest that high cyclin D1 expression could be a potential marker for tumor aggressiveness

Bcl-2 protein expression is associated with p27 and p53 protein expressions and MIB-1 counts in breast cancer

International Nuclear Information System (INIS)

Tsutsui, Shinichi; Yasuda, Kazuhiro; Suzuki, Kosuke; Takeuchi, Hideya; Nishizaki, Takashi; Higashi, Hidefumi; Era, Shoichi

2006-01-01

Recent experimental studies have shown that Bcl-2, which has been established as a key player in the control of apoptosis, plays a role in regulating the cell cycle and proliferation. The aim of this study was to investigate the relationship between Bcl-2 and p27 protein expression, p53 protein expression and the proliferation activity as defined by the MIB-1 counts. The prognostic implication of Bcl-2 protein expression in relation to p27 and p53 protein expressions and MIB-1 counts for breast cancer was also evaluated. The immunohistochemical expression of Bcl-2 protein was evaluated in a series of 249 invasive ductal carcinomas of the breast, in which p27 and p53 protein expressions and MIB-1 counts had been determined previously. The Bcl-2 protein expression was found to be decreased in 105 (42%) cases. A decreased Bcl-2 protein expression was significantly correlated with a nuclear grade of III, a negative estrogen receptor, a decreased p27 protein expression, a positive p53 protein expression, positive MIB-1 counts and a positive HER2 protein expression. The incidence of a nuclear grade of III and positive MIB-1 counts increased as the number of abnormal findings of Bcl-2, p27 and p53 protein expressions increased. A univariate analysis indicated a decreased Bcl-2 protein expression to be significantly (p = 0.0089) associated with a worse disease free survival (DFS), while a multivariate analysis indicated the lymph node status and MIB-1 counts to be independently significant prognostic factors for the DFS. The Bcl-2 protein expression has a close correlation with p27 and p53 protein expressions and the proliferation activity determined by MIB-1 counts in invasive ductal carcinoma of the breast. The prognostic value of Bcl-2 as well as p27 and p53 protein expressions was dependent on the proliferation activity in breast cancer

Bcl-2 protein expression is associated with p27 and p53 protein expressions and MIB-1 counts in breast cancer

Directory of Open Access Journals (Sweden)

Nishizaki Takashi

2006-07-01

Full Text Available Abstract Background Recent experimental studies have shown that Bcl-2, which has been established as a key player in the control of apoptosis, plays a role in regulating the cell cycle and proliferation. The aim of this study was to investigate the relationship between Bcl-2 and p27 protein expression, p53 protein expression and the proliferation activity as defined by the MIB-1 counts. The prognostic implication of Bcl-2 protein expression in relation to p27 and p53 protein expressions and MIB-1 counts for breast cancer was also evaluated. Methods The immunohistochemical expression of Bcl-2 protein was evaluated in a series of 249 invasive ductal carcinomas of the breast, in which p27 and p53 protein expressions and MIB-1 counts had been determined previously. Results The Bcl-2 protein expression was found to be decreased in 105 (42% cases. A decreased Bcl-2 protein expression was significantly correlated with a nuclear grade of III, a negative estrogen receptor, a decreased p27 protein expression, a positive p53 protein expression, positive MIB-1 counts and a positive HER2 protein expression. The incidence of a nuclear grade of III and positive MIB-1 counts increased as the number of abnormal findings of Bcl-2, p27 and p53 protein expressions increased. A univariate analysis indicated a decreased Bcl-2 protein expression to be significantly (p = 0.0089 associated with a worse disease free survival (DFS, while a multivariate analysis indicated the lymph node status and MIB-1 counts to be independently significant prognostic factors for the DFS. Conclusion The Bcl-2 protein expression has a close correlation with p27 and p53 protein expressions and the proliferation activity determined by MIB-1 counts in invasive ductal carcinoma of the breast. The prognostic value of Bcl-2 as well as p27 and p53 protein expressions was dependent on the proliferation activity in breast cancer.

PRAME gene expression profile in medulloblastoma

Directory of Open Access Journals (Sweden)

Tânia Maria Vulcani-Freitas

2011-02-01

Full Text Available Medulloblastoma is the most common malignant tumors of central nervous system in the childhood. The treatment is severe, harmful and, thus, has a dismal prognosis. As PRAME is present in various cancers, including meduloblastoma, and has limited expression in normal tissues, this antigen can be an ideal vaccine target for tumor immunotherapy. In order to find a potential molecular target, we investigated PRAME expression in medulloblastoma fragments and we compare the results with the clinical features of each patient. Analysis of gene expression was performed by real-time quantitative PCR from 37 tumor samples. The Mann-Whitney test was used to analysis the relationship between gene expression and clinical characteristics. Kaplan-Meier curves were used to evaluate survival. PRAME was overexpressed in 84% samples. But no statistical association was found between clinical features and PRAME overexpression. Despite that PRAME gene could be a strong candidate for immunotherapy since it is highly expressed in medulloblastomas.

Structural development of child's artistic expression

OpenAIRE

Sanja Filipović; Milica Vojvodić

2017-01-01

Abstract Structural development implies control and capability of the expression usage in terms of independent creative expression and making. Understanding of structural development of child's artistic expression as a phenomenon (which is suitable to child's age) has some implications on methodical acts considering artistic education of children and youngsters. Therefore, it is of unexceptional importance to know these laws as well as methodical acts which encourage the structural develop...

A system design for storing, archiving, and retrieving hyperspectral data

Science.gov (United States)

Dedecker, Ralph G.; Whittaker, Tom; Garcia, Raymond K.; Knuteson, Robert O.

2004-10-01

Hyperspectral data and products derived from instrumentation such as the Atmospheric Infrared Sounder (AIRS), the Cross-track Infrared Sounder (CrIS), Geosynchronous Imaging Fourier Transform Spectrometer (GIFTS) and the GOES-R Hyperspectral Environmental Suite (HES) will impose storage and data retrieval requirements that far exceed the demands of earlier generation remote sensing instrumentation used for atmospheric science research. A new architecture designed to address projected real time and research needs is undergoing prototype design and development. The system is designed using proven aspects of distributed data storage networks, descriptive metadata associated with stored files, data cataloging and database search schemes, and a data delivery approach that obeys accepted standards. Preliminary implementation and testing of some components of this architecture indicate that the design approach shows promise of an improved method for storage and library functionality for the data volumes associated with operational hyperspectral instrumentation.

The GEOS Chemistry Climate Model: Comparisons to Satellite Data

Science.gov (United States)

Stolarski, R. S.; Douglass, A. R.

2008-05-01

The Goddard Earth Observing System Chemistry Climate Model (GEOS CCM) has been developed by combining the atmospheric chemistry and transport modules developed over the years at Goddard and the GEOS general circulation model, also developed at Goddard. We will compare model simulations of ozone, and the minor constituents that affect ozone, for the period around 1980 with newly released revised data from the Limb Infrared Monitor of the Stratosphere (LIMS) instrument on Nimbus 4. We will also compare model simulations for the period of the early 2000s with the data from the Microwave Limb Sounder (MLS) and the High Resolution Dynamic Limb Sounder (HRDLS) on the Aura satellite. We will use these comparisons to examine the performance of the model for the present atmosphere and for the change that has occurred during the last 2 decades of ozone loss due to chlorine and bromine compounds released from chlorofluorocarbons and halons.

The expressions of emotions

DEFF Research Database (Denmark)

Vishnivetz, Berta

Abstract On the broadness of the vast field called “Expressions of Emotions” this study focuses on the whole bodily emotional expression. The main question posed is: Whether there are movement patterns specific to each emotion?. I carried out a thorough review of the theories of emotion...... and of expressions of emotions and movement notation that provided the sources for a careful research plan for the empirical process of this study. On this basis I chose to record onto video the four previously choreographed movements that I considered to correspond each of the following emotions: joy, fear, sadness......, anger. The selection of these four emotions demanded previously to clear up the problems the above named survey ensued. When researchers want to describe a certain movement in the field of psychology and non-verbal communication, it may result in disagreements and misunderstandings which sometimes lead...

Regulation of Expressive Behavior as Reflecting Affect Socialization.

Science.gov (United States)

Saarni, Carolyn

Regulated expressiveness (the modification of expressive behavior) is a complex phenomenon. Accomplished basically in four ways, regulated expressiveness has developmental dimensions, motivational precursors, and cognitive antecedents, including perspective-taking ability and the growth of self-awareness. Ability to regulate expressiveness appears…

Time perception and dynamics of facial expressions of emotions.

Directory of Open Access Journals (Sweden)

Sophie L Fayolle

Full Text Available Two experiments were run to examine the effects of dynamic displays of facial expressions of emotions on time judgments. The participants were given a temporal bisection task with emotional facial expressions presented in a dynamic or a static display. Two emotional facial expressions and a neutral expression were tested and compared. Each of the emotional expressions had the same affective valence (unpleasant, but one was high-arousing (expressing anger and the other low-arousing (expressing sadness. Our results showed that time judgments are highly sensitive to movements in facial expressions and the emotions expressed. Indeed, longer perceived durations were found in response to the dynamic faces and the high-arousing emotional expressions compared to the static faces and low-arousing expressions. In addition, the facial movements amplified the effect of emotions on time perception. Dynamic facial expressions are thus interesting tools for examining variations in temporal judgments in different social contexts.

Inferring gene expression dynamics via functional regression analysis

Directory of Open Access Journals (Sweden)

Leng Xiaoyan

2008-01-01

Full Text Available Abstract Background Temporal gene expression profiles characterize the time-dynamics of expression of specific genes and are increasingly collected in current gene expression experiments. In the analysis of experiments where gene expression is obtained over the life cycle, it is of interest to relate temporal patterns of gene expression associated with different developmental stages to each other to study patterns of long-term developmental gene regulation. We use tools from functional data analysis to study dynamic changes by relating temporal gene expression profiles of different developmental stages to each other. Results We demonstrate that functional regression methodology can pinpoint relationships that exist between temporary gene expression profiles for different life cycle phases and incorporates dimension reduction as needed for these high-dimensional data. By applying these tools, gene expression profiles for pupa and adult phases are found to be strongly related to the profiles of the same genes obtained during the embryo phase. Moreover, one can distinguish between gene groups that exhibit relationships with positive and others with negative associations between later life and embryonal expression profiles. Specifically, we find a positive relationship in expression for muscle development related genes, and a negative relationship for strictly maternal genes for Drosophila, using temporal gene expression profiles. Conclusion Our findings point to specific reactivation patterns of gene expression during the Drosophila life cycle which differ in characteristic ways between various gene groups. Functional regression emerges as a useful tool for relating gene expression patterns from different developmental stages, and avoids the problems with large numbers of parameters and multiple testing that affect alternative approaches.

Prognostic significance of snail expression in hilar cholangiocarcinoma

Energy Technology Data Exchange (ETDEWEB)

Kong, Dalu [Department of Hepatobiliary Surgery, Tianjin Medical University Cancer Institute and Hospital, Hexi District, Tianjin (China); Liang, Jun [Department of Oncology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province (China); Li, Rong [Department of Hepatobiliary Surgery, Tianjin Medical University Cancer Institute and Hospital, Hexi District, Tianjin (China); Liu, Shihai [Department of Laboratory Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province (China); Wang, Jigang [Department of Oncology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province (China); Zhang, Kejun; Chen, Dong [Department of General Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province (China)

2012-05-11

Many patients with hilar cholangiocarcinoma (HC) have a poor prognosis. Snail, a transcription factor and E-cadherin repressor, is a novel prognostic factor in many cancers. The aim of this study was to evaluate the relationship between snail and E-cadherin protein expression and the prognostic significance of snail expression in HC. We examined the protein expression of snail and E-cadherin in HC tissues from 47 patients (22 males and 25 females, mean age 61.2 years) using immunohistochemistry and RT-PCR. Proliferation rate was also evaluated in the same cases by the MIB1 index. High, low and negative snail protein expression was recorded in 18 (38%), 17 (36%), and 12 (26%) cases, respectively, and 40.4% (19/47) cases showed reduced E-cadherin protein expression in HC samples. No significant correlation was found between snail and E-cadherin protein expression levels (P = 0.056). No significant correlation was found between snail protein expression levels and gender, age, tumor grade, vascular or perineural invasion, nodal metastasis and invasion, or proliferative index. Cancer samples with positive snail protein expression were associated with poor survival compared with the negative expresser groups. Kaplan-Meier curves comparing different snail protein expression levels to survival showed highly significant separation (P < 0.0001, log-rank test). With multivariate analysis, only snail protein expression among all parameters was found to influence survival (P = 0.0003). We suggest that snail expression levels can predict poor survival regardless of pathological features and tumor proliferation. Immunohistochemical detection of snail protein expression levels in routine sections may provide the first biological prognostic marker.

The Arabidopsis co-expression tool (act): a WWW-based tool and database for microarray-based gene expression analysis

DEFF Research Database (Denmark)

Jen, C. H.; Manfield, I. W.; Michalopoulos, D. W.

2006-01-01

be examined using the novel clique finder tool to determine the sets of genes most likely to be regulated in a similar manner. In combination, these tools offer three levels of analysis: creation of correlation lists of co-expressed genes, refinement of these lists using two-dimensional scatter plots......We present a new WWW-based tool for plant gene analysis, the Arabidopsis Co-Expression Tool (act) , based on a large Arabidopsis thaliana microarray data set obtained from the Nottingham Arabidopsis Stock Centre. The co-expression analysis tool allows users to identify genes whose expression...

Positive Bioluminescence Imaging of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES.

Science.gov (United States)

Baril, Patrick; Pichon, Chantal

2016-01-01

MicroRNAs (miRNAs) are a class of small, noncoding RNAs which regulate gene expression by directing their target mRNA for degradation or translational repression. Since their discovery in the early 1990s, miRNAs have emerged as key components in the posttranscriptional regulation of gene networks, shaping many biological processes from development, morphogenesis, differentiation, proliferation and apoptosis. Although understanding of the molecular basis of miRNA biology is improving, methods to monitor the dynamic and the spatiotemporal aspects of miRNA expression under physiopathological conditions are required. However, monitoring of miRNAs is difficult due to their small size, low abundance, high degree of sequence similarity, and their dynamic expression pattern which is subjected to tight transcriptional and post-transcriptional controls. Recently, we developed a miRNA monitoring system called RILES, standing for RNAi-inducible expression system, which relies on an engineered regulatable expression system, to switch on the expression of the luciferase gene when the targeted miRNA is expressed in cells. We demonstrated that RILES is a specific, sensitive, and robust method to determine the fine-tuning of miRNA expression during the development of an experimental pathological process in mice. Because RILES offers the possibility for longitudinal studies on individual subjects, sharper insights into miRNA regulation can be generated, with applications in physiology, pathophysiology and development of RNAi-based therapies. This chapter describes methods and protocols to monitor the expression of myomiR-206, -1, and -133 in the tibialis anterior muscle of mice. These protocols can be used and adapted to monitor the expression of other miRNAs in other biological processes.

Expression of an Msx homeobox gene in ascidians: insights into the archetypal chordate expression pattern.

Science.gov (United States)

Ma, L; Swalla, B J; Zhou, J; Dobias, S L; Bell, J R; Chen, J; Maxson, R E; Jeffery, W R

1996-03-01

The Msx homeobox genes are expressed in complex patterns during vertebrate development in conjunction with inductive tissue interactions. As a means of understanding the archetypal role of Msx genes in chordates, we have isolated and characterized an Msx gene in ascidians, protochordates with a relatively simple body plan. The Mocu Msx-a and McMsx-a genes, isolated from the ascidians Molgula oculata and Molgula citrina, respectively, have homeodomains that place them in the msh-like subclass of Msx genes. Therefore, the Molgula Msx-a genes are most closely related to the msh genes previously identified in a number of invertebrates. Southern blot analysis suggests that there are one or two copies of the Msx-a gene in the Molgula genome. Northern blot and RNase protection analysis indicate that Msx-a transcripts are restricted to the developmental stages of the life cycle. In situ hybridization showed that Msx-a mRNA first appears just before gastrulation in the mesoderm (presumptive notochord and muscle) and ectoderm (neural plate) cells. Transcript levels decline in mesoderm cells after the completion of gastrulation, but are enhanced in the folding neural plate during neurulation. Later, Msx-a mRNA is also expressed in the posterior ectoderm and in a subset of the tail muscle cells. The ectoderm and mesoderm cells that express Msx-a are undergoing morphogenetic movements during gastrulation, neurulation, and tail formation. Msx-a expression ceases after these cells stop migrating. The ascidian M. citrina, in which adult tissues and organs begin to develop precociously in the larva, was used to study Msx-a expression during adult development. Msx-a transcripts are expressed in the heart primordium and the rudiments of the ampullae, epidermal protrusions with diverse functions in the juvenile. The heart and ampullae develop in regions where mesenchyme cells interact with endodermal or epidermal epithelia. A comparison of the expression patterns of the Molgula genes

Lysine-specific demethylase 2A expression is associated with cell growth and cyclin D1 expression in colorectal adenocarcinoma.

Science.gov (United States)

Cao, Lin-Lin; Du, Changzheng; Liu, Hangqi; Pei, Lin; Qin, Li; Jia, Mei; Wang, Hui

2018-04-01

Lysine-specific demethylase 2A (KDM2A), a specific H3K36me1/2 demethylase, has been reported to be closely associated with several types of cancer. In this study, we aimed to investigate the expression and function of KDM2A in colorectal adenocarcinoma. A total of 215 colorectal adenocarcinoma specimens were collected, and then subjected to immunohistochemistry assay to evaluate the expression levels of KDM2A, cyclin D1 and other proteins in colorectal adenocarcinoma tissues. Real-time polymerase chain reaction, Western blot, and other molecular biology methods were used to explore the role of KDM2A in colorectal adenocarcinoma cells. In this study, we report that the expression level of KDM2A is high in colorectal adenocarcinoma tissues, and this high expression promotes the proliferation and colony formation of colorectal adenocarcinoma cells, as demonstrated by KDM2A knockdown experiments. In addition, the expression of KDM2A is closely associated with cyclin D1 expression in colorectal adenocarcinoma tissues and cell lines. Our study reveals a novel role for high-expressed KDM2A in colorectal adenocarcinoma cell growth, and that the expression of KDM2A is associated with that of cyclin D1 in colorectal adenocarcinoma.

Expression profile of the Schistosoma japonicum degradome reveals differential protease expression patterns and potential anti-schistosomal intervention targets.

Directory of Open Access Journals (Sweden)

Shuai Liu

2014-10-01

Full Text Available Blood fluke proteases play pivotal roles in the processes of invasion, nutrition acquisition, immune evasion, and other host-parasite interactions. Hundreds of genes encoding putative proteases have been identified in the recently published schistosome genomes. However, the expression profiles of these proteases in Schistosoma species have not yet been systematically analyzed. We retrieved and culled the redundant protease sequences of Schistosoma japonicum, Schistosoma mansoni, Echinococcus multilocularis, and Clonorchis sinensis from public databases utilizing bioinformatic approaches. The degradomes of the four parasitic organisms and Homo sapiens were then comparatively analyzed. A total of 262 S. japonicum protease sequences were obtained and the expression profiles generated using whole-genome microarray. Four main clusters of protease genes with different expression patterns were identified: proteases up-regulated in hepatic schistosomula and adult worms, egg-specific or predominantly expressed proteases, cercaria-specific or predominantly expressed proteases, and constantly expressed proteases. A subset of protease genes with different expression patterns were further validated using real-time quantitative PCR. The present study represents the most comprehensive analysis of a degradome in Schistosoma species to date. These results provide a firm foundation for future research on the specific function(s of individual proteases and may help to refine anti-proteolytic strategies in blood flukes.

Behavioral experience induces zif268 expression in mature granule cells but suppresses its expression in immature granule cells

Directory of Open Access Journals (Sweden)

Kylie A. Huckleberry

2015-08-01

Full Text Available Thousands of neurons are born each day in the dentate gyrus (DG, but many of these cells die before reaching maturity. Both death and survival of adult-born neurons are regulated by neuronal activity in DG. The immediate-early gene (IEG zif268 is an important mediator of these effects, as its expression is induced by neural activity and knockout of zif268 impairs survival of adult-born neurons (Veyrac et al., 2013. Despite the apparent importance of zif268 for adult neurogenesis, its behavior-induced expression has not been fully characterized in adult-born neurons. Here we characterize behavior-evoked expression of zif268 in mature and newborn dentate granule cells (DGCs. In the general granule cell population, zif268 expression peaked 1 hour after novel environment exposure and returned to baseline by 8 hours post-exposure. However, in the doublecortin-positive (DCX+ immature neurons, zif268 expression was suppressed relative to home cage for at least 8 hours post-exposure. We next determined that exposure to water maze training, an enriched environment, or a novel environment caused approximately equal suppression of zif268 expression in DCX+ cells and approximately equal activation of zif268 in the general DGC population and in 6-week-old adult-born neurons. Finally, we asked whether zif268 suppression varied as a function of age within the DCX+ population, which ranges in age from 0 to approximately 4 weeks. Novel environment exposure had no significant effect on zif268 expression in 2- or 4-week-old BrdU-labeled neurons, but it significantly suppressed zif268 expression in 3-week-old neurons. In summary, behavioral experience transiently activated expression of zif268 in mature DGCs but caused a more long-lasting suppression of zif268 expression in immature, adult-born DGCs. We hypothesize that zif268 suppression inhibits memory-related synaptic plasticity in immature DGCs or mediates learning-induced apoptosis of immature adult

75 FR 80561 - Community Express Pilot Program

Science.gov (United States)

2010-12-22

... SMALL BUSINESS ADMINISTRATION Community Express Pilot Program AGENCY: U.S. Small Business Administration (SBA). ACTION: Notice of short-term extension and termination of the Community Express Pilot Program. SUMMARY: This notice announces the termination of the Community Express Pilot Program following a...

HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression.

Science.gov (United States)

Gomes, Renan Garcia; Brito, Carlos Alexandre Antunes de; Martinelli, Valéria Ferreira; Santos, Rossana Nascimento Dos; Gomes, Fabiana Oliveira Dos Santos; Peixoto, Christina Alves; Crispim, Janaína Oliveira; Diniz, George Tadeu Nunes; Donadi, Eduardo Antônio; Lucena-Silva, Norma

2018-06-01

HLA-G is an immunomodulatory molecule that can be produced by epithelial cells. Considering that TNF and IL-10 participate in bowel inflammatory disorders and that both cytokines modulate HLA-G, we evaluated HLA-G, TNF and IL-10 mRNA expression by qPCR and HLA-G protein levels by immunohistochemistry in two intestinal samples exhibiting different degree of inflammation within a patient suffering from Crohn's disease (CD) or ulcerative colitis (UC). Tissue HLA-G5 (P < 0.0001), TNF (P = 0.0004) and IL-10 (P = 0.0169) mRNA expression levels were higher in intestinal areas exhibiting intense inflammation compared to areas of low inflammation, and HLA-G protein levels were not associated with degree of mucosal inflammation. In CD, the expression of TNF was correlated with IL-10 in low inflamed areas, exhibiting a TNF:IL-10 ratio = 3, but in inflamed areas the ratio increased to 9-folds. In UC, the expression of TNF was correlated to IL-10, irrespective of the inflammation grade, with little variation of the TNF:IL-10 ratio in the various inflamed areas. TNF and IL-10 expression was correlated with HLA-G5 expression in mild inflamed areas. Both CD and UC samples exhibited gene and protein expression of HLA-G; and the HLA-G5 expression is differentially correlated with TNF and IL-10 levels depending on the type of the underlying inflammatory bowel disorder. Copyright © 2018 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Edix: A Software for Editing Algebraic Expressions.

OpenAIRE

Bouhineau , Denis; Nicaud , Jean-François; Pavard , X.

2001-01-01

International audience; The paper presents a computer software, called Edix, devoted to the edition of algebraic expressions in their usual 2D representation. At present, many systems display fine algebraic expressions, but the edition of such expressions is weak. Systems like Word and FrameMaker place sub-expressions in too many boxes so that many editing actions are not simple, while usual CAS (computer algebra systems) just use a 1D representation for the edition. Furthermore, Edix allows ...

Kivy and Langer on expressiveness in music

Directory of Open Access Journals (Sweden)

van der Schoot Albert

2013-01-01

Full Text Available From 1980 onwards, Peter Kivy has put forward that music does not so much express emotions but rather is expressive of emotions. The character of the music does not represent the character or mood of the composer, but reflects his knowledge of emotional life. Unfortunately, Kivy fails to give credit to Susanne Langer, who brought these views to the fore as early as 1942, claiming that the vitality of music lies in expressiveness, not in expression.

VMEXT: A Visualization Tool for Mathematical Expression Trees

OpenAIRE

Schubotz, Moritz; Meuschke, Norman; Hepp, Thomas; Cohl, Howard S.; Gipp, Bela

2017-01-01

Mathematical expressions can be represented as a tree consisting of terminal symbols, such as identifiers or numbers (leaf nodes), and functions or operators (non-leaf nodes). Expression trees are an important mechanism for storing and processing mathematical expressions as well as the most frequently used visualization of the structure of mathematical expressions. Typically, researchers and practitioners manually visualize expression trees using general-purpose tools. This approach is labori...

Expression of Sox genes in tooth development.

Science.gov (United States)

Kawasaki, Katsushige; Kawasaki, Maiko; Watanabe, Momoko; Idrus, Erik; Nagai, Takahiro; Oommen, Shelly; Maeda, Takeyasu; Hagiwara, Nobuko; Que, Jianwen; Sharpe, Paul T; Ohazama, Atsushi

2015-01-01

Members of the Sox gene family play roles in many biological processes including organogenesis. We carried out comparative in situ hybridization analysis of seventeen sox genes (Sox1-14, 17, 18, 21) during murine odontogenesis from the epithelial thickening to the cytodifferentiation stages. Localized expression of five Sox genes (Sox6, 9, 13, 14 and 21) was observed in tooth bud epithelium. Sox13 showed restricted expression in the primary enamel knots. At the early bell stage, three Sox genes (Sox8, 11, 17 and 21) were expressed in pre-ameloblasts, whereas two others (Sox5 and 18) showed expression in odontoblasts. Sox genes thus showed a dynamic spatio-temporal expression during tooth development.

Bathymetry and Near-River Topography of the Naches and Yakima Rivers at Union Gap and Selah Gap, Yakima County, Washington, August 2008

Science.gov (United States)

Mastin, M.C.; Fosness, R.L.

2009-01-01

Yakima County is collaborating with the Bureau of Reclamation on a study of the hydraulics and sediment-transport in the lower Naches River and in the Yakima River between Union Gap and Selah Gap in Washington. River bathymetry and topographic data of the river channels are needed for the study to construct hydraulic models. River survey data were available for most of the study area, but river bathymetry and near-river topography were not available for Selah Gap, near the confluence of the Naches and Yakima Rivers, and for Union Gap. In August 2008, the U.S. Geological Survey surveyed the areas where data were not available. If possible, the surveys were made with a boat-mounted, single-beam echo sounder attached to a survey-grade Real-Time Kinematic (RTK) global positioning system (GPS). An RTK GPS rover was used on a walking survey of the river banks, shallow river areas, and river bed areas that were impenetrable to the echo sounder because of high densities of macrophytes. After the data were edited, 95,654 bathymetric points from the boat survey with the echo sounder and 1,069 points from the walking survey with the GPS rover were used in the study. The points covered 4.6 kilometers on the Yakima River and 0.6 kilometers on the Naches River. GPS-surveyed points checked within 0.014 to 0.047 meters in the horizontal direction and -0.036 to 0.078 meters in the vertical direction compared to previously established survey control points

75 FR 473 - Community Express Pilot Program

Science.gov (United States)

2010-01-05

... SMALL BUSINESS ADMINISTRATION Community Express Pilot Program AGENCY: U.S. Small Business Administration (SBA). ACTION: Notice of extension of the Community Express Pilot Program. SUMMARY: This notice extends the Community Express Pilot Program in its current form through December 31, 2010. Based upon the...

Foxp3 expression in human cancer cells

Directory of Open Access Journals (Sweden)

Gourgoulianis Konstantinos I

2008-04-01

Full Text Available Abstract Objective Transcription factor forkhead box protein 3 (Foxp3 specifically characterizes the thymically derived naturally occurring regulatory T cells (Tregs. Limited evidence indicates that it is also expressed, albeit to a lesser extent, in tissues other than thymus and spleen, while, very recently, it was shown that Foxp3 is expressed by pancreatic carcinoma. This study was scheduled to investigate whether expression of Foxp3 transcripts and mature protein occurs constitutively in various tumor types. Materials and methods Twenty five tumor cell lines of different tissue origins (lung cancer, colon cancer, breast cancer, melanoma, erythroid leukemia, acute T-cell leukemia were studied. Detection of Foxp3 mRNA was performed using both conventional RT-PCR and quantitative real-time PCR while protein expression was assessed by immunocytochemistry and flow cytometry, using different antibody clones. Results Foxp3 mRNA as well as Foxp3 protein was detected in all tumor cell lines, albeit in variable levels, not related to the tissue of origin. This expression correlated with the expression levels of IL-10 and TGFb1. Conclusion We offer evidence that Foxp3 expression, characterizes tumor cells of various tissue origins. The biological significance of these findings warrants further investigation in the context of tumor immune escape, and especially under the light of current anti-cancer efforts interfering with Foxp3 expression.

Change of SPARC expression after chemotherapy in gastric cancer

International Nuclear Information System (INIS)

Gao, Yong-Yin; Han, Ru-Bing; Wang, Xia; Ge, Shao-Hua; Li, Hong-Li; Deng, Ting; Liu, Rui; Bai, Ming; Zhou, Li-Kun; Zhang, Xin-Yuan; Ba, Yi; Huang, Ding-Zhi

2015-01-01

The expression of tumor biomarkers may change after chemotherapy. However, whether secreted protein acidic and rich in cysteine (SPARC) expression changes after chemotherapy in gastric cancer (GC) is unclear. This study investigated the influence of chemotherapy on SPARC expression in GC. Immunohistochemistry was used to analyze SPARC expression in 132 GC cases (including 54 cases with preoperative chemotherapy and 78 cases without preoperative chemotherapy). SPARC expression of postoperative specimens with and without preoperative chemotherapy was assessed to analyze the influence of chemotherapy on SPARC expression. SPARC was highly expressed in GC compared with the desmoplastic stroma surrounding tumor cells and noncancerous tissues. High SPARC expression was correlated with invasion depth, lymph node, and TNM stage. After chemotherapy, a lower proportion of high SPARC expression was observed in patients with preoperative chemotherapy than in the controls. For 54 patients with preoperative chemotherapy, gross type, histology, depth of invasion, lymph node, TNM stage, and SPARC expression were related to overall survival. Further multivariate analysis showed that lymph node, histology, and SPARC expression after chemotherapy were independent prognostic factors. SPARC expression may change after chemotherapy in GC. SPARC expression should be reassessed for patients with GC after chemotherapy

Functional features of gene expression profiles differentiating gastrointestinal stromal tumours according to KIT mutations and expression

International Nuclear Information System (INIS)

Ostrowski, Jerzy; Dobosz, Anna Jerzak Vel; Jarosz, Dorota; Ruka, Wlodzimierz; Wyrwicz, Lucjan S; Polkowski, Marcin; Paziewska, Agnieszka; Skrzypczak, Magdalena; Goryca, Krzysztof; Rubel, Tymon; Kokoszyñska, Katarzyna; Rutkowski, Piotr; Nowecki, Zbigniew I

2009-01-01

Gastrointestinal stromal tumours (GISTs) represent a heterogeneous group of tumours of mesenchymal origin characterized by gain-of-function mutations in KIT or PDGFRA of the type III receptor tyrosine kinase family. Although mutations in either receptor are thought to drive an early oncogenic event through similar pathways, two previous studies reported the mutation-specific gene expression profiles. However, their further conclusions were rather discordant. To clarify the molecular characteristics of differentially expressed genes according to GIST receptor mutations, we combined microarray-based analysis with detailed functional annotations. Total RNA was isolated from 29 frozen gastric GISTs and processed for hybridization on GENECHIP ® HG-U133 Plus 2.0 microarrays (Affymetrix). KIT and PDGFRA were analyzed by sequencing, while related mRNA levels were analyzed by quantitative RT-PCR. Fifteen and eleven tumours possessed mutations in KIT and PDGFRA, respectively; no mutation was found in three tumours. Gene expression analysis identified no discriminative profiles associated with clinical or pathological parameters, even though expression of hundreds of genes differentiated tumour receptor mutation and expression status. Functional features of genes differentially expressed between the two groups of GISTs suggested alterations in angiogenesis and G-protein-related and calcium signalling. Our study has identified novel molecular elements likely to be involved in receptor-dependent GIST development and allowed confirmation of previously published results. These elements may be potential therapeutic targets and novel markers of KIT mutation status

Differential expression and interaction of host factors augment HIV-1 gene expression in neonatal mononuclear cells

International Nuclear Information System (INIS)

Sundaravaradan, Vasudha; Mehta, Roshni; Harris, David T.; Zack, Jerome A.; Ahmad, Nafees

2010-01-01

We have previously shown a higher level of HIV-1 replication and gene expression in neonatal (cord) blood mononuclear cells (CBMC) compared with adult blood cells (PBMC), which could be due to differential expression of host factors. We performed the gene expression profile of CBMC and PBMC and found that 8013 genes were expressed at higher levels in CBMC than PBMC and 8028 genes in PBMC than CBMC, including 1181 and 1414 genes upregulated after HIV-1 infection in CBMC and PBMC, respectively. Several transcription factors (NF-κB, E2F, HAT-1, TFIIE, Cdk9, Cyclin T1), signal transducers (STAT3, STAT5A) and cytokines (IL-1β, IL-6, IL-10) were upregulated in CBMC than PBMC, which are known to influence HIV-1 replication. In addition, a repressor of HIV-1 transcription, YY1, was down regulated in CBMC than PBMC and several matrix metalloproteinase (MMP-7, -12, -14) were significantly upregulated in HIV-1 infected CBMC than PBMC. Furthermore, we show that CBMC nuclear extracts interacted with a higher extent to HIV-1 LTR cis-acting sequences, including NF-κB, NFAT, AP1 and NF-IL6 compared with PBMC nuclear extracts and retroviral based short hairpin RNA (shRNA) for STAT3 and IL-6 down regulated their own and HIV-1 gene expression, signifying that these factors influenced differential HIV-1 gene expression in CBMC than PBMC.

The Communicative Function of Sad Facial Expressions.

Science.gov (United States)

Reed, Lawrence Ian; DeScioli, Peter

2017-01-01

What are the communicative functions of sad facial expressions? Research shows that people feel sadness in response to losses but it's unclear whether sad expressions function to communicate losses to others and if so, what makes these signals credible. Here we use economic games to test the hypothesis that sad expressions lend credibility to claims of loss. Participants play the role of either a proposer or recipient in a game with a fictional backstory and real monetary payoffs. The proposers view a (fictional) video of the recipient's character displaying either a neutral or sad expression paired with a claim of loss. The proposer then decided how much money to give to the recipient. In three experiments, we test alternative theories by using situations in which the recipient's losses were uncertain (Experiment 1), the recipient's losses were certain (Experiment 2), or the recipient claims failed gains rather than losses (Experiment 3). Overall, we find that participants gave more money to recipients who displayed sad expressions compared to neutral expressions, but only under conditions of uncertain loss. This finding supports the hypothesis that sad expressions function to increase the credibility of claims of loss.

The Communicative Function of Sad Facial Expressions

Directory of Open Access Journals (Sweden)

Lawrence Ian Reed

2017-03-01

Full Text Available What are the communicative functions of sad facial expressions? Research shows that people feel sadness in response to losses but it’s unclear whether sad expressions function to communicate losses to others and if so, what makes these signals credible. Here we use economic games to test the hypothesis that sad expressions lend credibility to claims of loss. Participants play the role of either a proposer or recipient in a game with a fictional backstory and real monetary payoffs. The proposers view a (fictional video of the recipient’s character displaying either a neutral or sad expression paired with a claim of loss. The proposer then decided how much money to give to the recipient. In three experiments, we test alternative theories by using situations in which the recipient’s losses were uncertain (Experiment 1, the recipient’s losses were certain (Experiment 2, or the recipient claims failed gains rather than losses (Experiment 3. Overall, we find that participants gave more money to recipients who displayed sad expressions compared to neutral expressions, but only under conditions of uncertain loss. This finding supports the hypothesis that sad expressions function to increase the credibility of claims of loss.

The evolution of gene expression in primates

OpenAIRE

Tashakkori Ghanbarian, Avazeh

2015-01-01

The evolution of a gene’s expression profile is commonly assumed to be independent of its genomic neighborhood. This is, however, in contrast to what we know about the lack of autonomy between expression of neighboring genes in extant taxa. Indeed, in all eukaryotic genomes, genes of similar expression-profile tend to cluster, reflecting chromatin level dynamics. Does it follow that if a gene increases expression in a particular lineage then the genomic neighbors will also increase in their e...

Anger Expression Types and Interpersonal Problems in Nurses.

Science.gov (United States)

Han, Aekyung; Won, Jongsoon; Kim, Oksoo; Lee, Sang E

2015-06-01

The purpose of this study was to investigate the anger expression types in nurses and to analyze the differences between the anger expression types and interpersonal problems. The data were collected from 149 nurses working in general hospitals with 300 beds or more in Seoul or Gyeonggi province, Korea. For anger expression type, the anger expression scale from the Korean State-Trait Anger Expression Inventory was used. For interpersonal problems, the short form of the Korean Inventory of Interpersonal Problems Circumplex Scales was used. Data were analyzed using descriptive statistics, cluster analysis, multivariate analysis of variance, and Duncan's multiple comparisons test. Three anger expression types in nurses were found: low-anger expression, anger-in, and anger-in/control type. From the results of multivariate analysis of variance, there were significant differences between anger expression types and interpersonal problems (Wilks lambda F = 3.52, p interpersonal problems by Duncan's post hoc test (p interpersonal problems. Copyright © 2015. Published by Elsevier B.V.

SOX4 expression in bladder carcinoma

DEFF Research Database (Denmark)

Aaboe, Mads; Birkenkamp-Demtroder, Karin; Wiuf, Carsten

2006-01-01

The human transcription factor SOX4 was 5-fold up-regulated in bladder tumors compared with normal tissue based on whole-genome expression profiling of 166 clinical bladder tumor samples and 27 normal urothelium samples. Using a SOX4-specific antibody, we found that the cancer cells expressed...... in the clinical bladder material and a small subset of the genes showed a high correlation to SOX4 expression. The present data suggest a role of SOX4 in the bladder cancer disease....... the SOX4 protein and, thus, did an evaluation of SOX4 protein expression in 2,360 bladder tumors using a tissue microarray with clinical annotation. We found a correlation (P bladder cell line HU609, SOX4...

Paradoxical expression of INK4c in proliferative multiple myeloma tumors: bi-allelic deletion vs increased expression

Directory of Open Access Journals (Sweden)

Hanamura Ichiro

2006-10-01

Full Text Available Abstract Background A high proliferative capacity of tumor cells usually is associated with shortened patient survival. Disruption of the RB pathway, which is critically involved in regulating the G1 to S cell cycle transition, is a frequent target of oncogenic events that are thought to contribute to increased proliferation during tumor progression. Previously, we determined that p18INK4c, an essential gene for normal plasma cell differentiation, was bi-allelically deleted in five of sixteen multiple myeloma (MM cell lines. The present study was undertaken to investigate a possible role of p18INK4c in increased proliferation of myeloma tumors as they progress. Results Thirteen of 40 (33% human myeloma cell lines do not express normal p18INK4c, with bi-allelic deletion of p18 in twelve, and expression of a mutated p18 fragment in one. Bi-allelic deletion of p18, which appears to be a late progression event, has a prevalence of about 2% in 261 multiple myeloma (MM tumors, but the prevalence is 6 to10% in the 50 tumors with a high expression-based proliferation index. Paradoxically, 24 of 40 (60% MM cell lines, and 30 of 50 (60% MM tumors with a high proliferation index express an increased level of p18 RNA compared to normal bone marrow plasma cells, whereas this occurs in only five of the 151 (3% MM tumors with a low proliferation index. Tumor progression is often accompanied by increased p18 expression and an increased proliferation index. Retroviral-mediated expression of exogenous p18 results in marked growth inhibition in three MM cell lines that express little or no endogenous p18, but has no effect in another MM cell line that already expresses a high level of p18. Conclusion Paradoxically, although loss of p18 appears to contribute to increased proliferation of nearly 10% of MM tumors, most MM cell lines and proliferative MM tumors have increased expression of p18. Apart from a small fraction of cell lines and tumors that have inactivated

Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

International Nuclear Information System (INIS)

Pinho, Salomé S; Matos, Augusto JF; Lopes, Célia; Marcos, Nuno T; Carvalheira, Júlio; Reis, Celso A; Gärtner, Fátima

2007-01-01

Sialyl Lewis x (sLe x ) antigen is a carbohydrate antigen that is considered not only a marker for cancer but also implicated functionally in the malignant behaviour of cancer cells. Overexpression of sLe x is associated with enhanced progression and metastases of many types of cancer including those of the mammary gland. Canine mammary tumours can invade and give rise to metastases via either lymphatic or blood vessels. E-Cadherin is specifically involved in epithelial cell-to-cell adhesion. In cancer, E-Cadherin underexpression is one of the alterations that characterizes the invasive phenotype and is considered an invasion/tumour suppressor gene. Partial or complete loss of E-Cadherin expression correlates with poor prognosis in canine malignant mammary cancer. The aim of this study was to analyse the sLe x expression in canine malignant mammary tumours and to evaluate if the presence of sLe x correlates with the expression of E-Cadherin and with clinicopathological features. Fifty-three cases of canine mammary carcinomas were analysed immunohistochemically using monoclonal antibodies against sLe x (IgM) and E-Cadherin (IgG). The clinicopathological data were then assessed to determine whether there was a correlation with sLe x tumour expression. Double labelled immunofluorescence staining was performed to analyse the combined expression of sLe x and E-Cadherin. sLe x expression was consistently demonstrated in all cases of canine mammary carcinomas with different levels of expression. We found a significant relationship between the levels of sLe x expression and the presence of lymph node metastases. We also demonstrated that when E-Cadherin expression was increased sLe x was reduced and vice-versa. The combined analysis of both adhesion molecules revealed an inverse relationship. In the present study we demonstrate the importance of sLe x in the malignant phenotype of canine malignant mammary tumours. Our results support the use of sLe x as a prognostic tumour

Generating Expressive Speech for Storytelling Applications

OpenAIRE

Bailly, G.; Theune, Mariet; Meijs, Koen; Campbell, N.; Hamza, W.; Heylen, Dirk K.J.; Ordelman, Roeland J.F.; Hoge, H.; Jianhua, T.

2006-01-01

Work on expressive speech synthesis has long focused on the expression of basic emotions. In recent years, however, interest in other expressive styles has been increasing. The research presented in this paper aims at the generation of a storytelling speaking style, which is suitable for storytelling applications and more in general, for applications aimed at children. Based on an analysis of human storytellers' speech, we designed and implemented a set of prosodic rules for converting "neutr...

Methods for monitoring multiple gene expression

Energy Technology Data Exchange (ETDEWEB)

Berka, Randy [Davis, CA; Bachkirova, Elena [Davis, CA; Rey, Michael [Davis, CA

2012-05-01

The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.